primer3-2.3.6/0000775000077300007730000000000012231156530012053 5ustar ioanaioanaprimer3-2.3.6/cmp_settings.pl0000775000077300007730000000664112231156245015124 0ustar ioanaioana#!/usr/bin/perl # # Logical compare (i.e. diff) of two settings files. # # For usage, see the usage statement in the code, below. # # ====================================================================== # (c) Copyright 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2010, # 2011,2012 # Whitehead Institute for Biomedical Research, Steve Rozen, # Andreas Untergasser and Helen Skaletsky # All rights reserved. # # This file is part of the primer3 suite. # # The primer3 suite is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License as # published by the Free Software Foundation; either version 2 of the # License, or (at your option) any later version. # # This software is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # # You should have received a copy of the GNU General Public License # along with this file (file gpl-2.0.txt in the source distribution); if # not, write to the Free Software Foundation, Inc., 51 Franklin St, # Fifth Floor, Boston, MA 02110-1301 USA # # THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS # "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT # LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR # A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT # OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, # SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT # LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, # DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY # THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT # (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE # OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. # ====================================================================== use strict; use warnings "all"; use Getopt::Long; my $file1; my $file2; my %tags1; my %tags2; sub read_file($$) { my ($file, $tags) = @_; open IN, $file or die "open $file: $!\n"; # skip first line my $line = ; while ($line = ) { chomp($line); # skip empty lines and comments if ($line =~ /^\s*$/) { next; } if ($line =~ "^#") { next; } unless ($line =~ /(\S*)=(.*)/) { print STDERR "wrong line format: $line\n"; next; } my $tag = $1; my $value = $2; $tags->{$tag} = $value; } close IN; } if (!GetOptions("file1=s" => \$file1, "file2=s" => \$file2) || !defined($file1) || !defined($file2)) { die "\nUsage:\n" . "$0 -file1 -file2 \n"; } read_file($file1, \%tags1); read_file($file2, \%tags2); my @only1; # cmp tags1 with tags2 print anything common different print "Common tags with different values:\n"; foreach my $tag (sort (keys %tags1)) { if (defined($tags2{$tag})) { if ($tags1{$tag} ne $tags2{$tag}) { print "\t$tag:\n\t\t$file1: $tag=$tags1{$tag}\n\t\t$file2: $tag=$tags2{$tag}\n" } } else { push(@only1, $tag); } } print "Tags that exist only in $file1:\n"; foreach my $tag (@only1) { print "\t$tag=$tags1{$tag}\n"; } print "Tags that exist only in $file2:\n"; foreach my $tag (keys %tags2) { if (!defined($tags1{$tag})) { print "\t$tag=$tags2{$tag}\n"; } } primer3-2.3.6/example0000664000077300007730000000074012231156245013435 0ustar ioanaioanaSEQUENCE_ID=example SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_TASK=pick_detection_primers PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 PRIMER_PRODUCT_SIZE_RANGE=75-100 P3_FILE_FLAG=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_EXPLAIN_FLAG=1 = primer3-2.3.6/primer3web_v0_4_0_default_settings.txt0000664000077300007730000000656312231156245021403 0ustar ioanaioanaPrimer3 File - http://primer3.sourceforge.net P3_FILE_TYPE=settings P3_FILE_ID=Default settings of primer3web version 0.4.0 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=0 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0 PRIMER_TASK=pick_detection_primers PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=5 PRIMER_PRODUCT_SIZE_RANGE=150-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000 PRIMER_PRODUCT_OPT_SIZE=0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_MIN_SIZE=18 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_MAX_SIZE=27 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_MIN_GC=20.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_MAX_GC=80.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_GC_CLAMP=0 PRIMER_MAX_END_GC=5 PRIMER_MIN_TM=57.0 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_MAX_TM=63.0 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_PAIR_MAX_DIFF_TM=100.0 PRIMER_WT_TM_LT=1.0 PRIMER_INTERNAL_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PRODUCT_MIN_TM=-1000000.0 PRIMER_PRODUCT_OPT_TM=0.0 PRIMER_PRODUCT_MAX_TM=1000000.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_TM_FORMULA=0 PRIMER_SALT_MONOVALENT=50.0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=0.0 PRIMER_INTERNAL_SALT_DIVALENT=0.0 PRIMER_DNTP_CONC=0.0 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_SALT_CORRECTIONS=0 PRIMER_DNA_CONC=50.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_MAX_SELF_ANY=8.00 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_WT_SELF_ANY=0.0 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_MAX_SELF_END=3.00 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_WT_SELF_END=0.0 PRIMER_INTERNAL_WT_SELF_END=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_MAX_END_STABILITY=9.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_MAX_POLY_X=5 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_MIN_THREE_PRIME_DISTANCE=-1 PRIMER_PICK_ANYWAY=1 PRIMER_LOWERCASE_MASKING=0 PRIMER_EXPLAIN_FLAG=1 PRIMER_LIBERAL_BASE=1 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MAX_TEMPLATE_MISPRIMING=12.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=24.00 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=24.00 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_MIN_QUALITY=0 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_MIN_END_QUALITY=0 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_QUALITY_RANGE_MAX=100 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_INSIDE_PENALTY=-1.0 PRIMER_OUTSIDE_PENALTY=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_SEQUENCING_LEAD=50 PRIMER_SEQUENCING_SPACING=500 PRIMER_SEQUENCING_INTERVAL=250 PRIMER_SEQUENCING_ACCURACY=20 PRIMER_WT_END_QUAL=0.0 PRIMER_INTERNAL_WT_END_QUAL=0.0 = primer3-2.3.6/primer3_v1_1_4_default_settings.txt0000664000077300007730000000640312231156245020700 0ustar ioanaioanaPrimer3 File - http://primer3.sourceforge.net P3_FILE_TYPE=settings P3_FILE_ID=Default settings of primer3 version 1.1.4 PRIMER_TASK=pick_detection_primers PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=5 PRIMER_PRODUCT_SIZE_RANGE=100-300 PRIMER_PRODUCT_OPT_SIZE=0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_MIN_SIZE=18 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_MAX_SIZE=27 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_MIN_GC=20.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_OPT_GC_PERCENT=50.0 PRIMER_MAX_GC=80.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_GC_CLAMP=0 PRIMER_MAX_END_GC=5 PRIMER_MIN_TM=57.0 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_MAX_TM=63.0 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_PAIR_MAX_DIFF_TM=100.0 PRIMER_WT_TM_LT=1.0 PRIMER_INTERNAL_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PRODUCT_MIN_TM=-1000000.0 PRIMER_PRODUCT_OPT_TM=0.0 PRIMER_PRODUCT_MAX_TM=1000000.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_TM_FORMULA=0 PRIMER_SALT_MONOVALENT=50.0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=0.0 PRIMER_INTERNAL_SALT_DIVALENT=0.0 PRIMER_DNTP_CONC=0.0 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_SALT_CORRECTIONS=0 PRIMER_DNA_CONC=50.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_MAX_SELF_ANY=8.00 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_WT_SELF_ANY=0.0 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_MAX_SELF_END=3.00 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_WT_SELF_END=0.0 PRIMER_INTERNAL_WT_SELF_END=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_MAX_END_STABILITY=100.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_MAX_POLY_X=5 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_MIN_THREE_PRIME_DISTANCE=-1 PRIMER_PICK_ANYWAY=0 PRIMER_LOWERCASE_MASKING=0 PRIMER_EXPLAIN_FLAG=0 PRIMER_LIBERAL_BASE=0 PRIMER_FIRST_BASE_INDEX=0 PRIMER_MAX_TEMPLATE_MISPRIMING=-1.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=-1.00 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=24.00 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_MIN_QUALITY=0 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_MIN_END_QUALITY=0 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_QUALITY_RANGE_MAX=100 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_INSIDE_PENALTY=-1.0 PRIMER_OUTSIDE_PENALTY=0.0 PRIMER_WT_POS_PENALTY=1.0 PRIMER_SEQUENCING_LEAD=50 PRIMER_SEQUENCING_SPACING=500 PRIMER_SEQUENCING_INTERVAL=250 PRIMER_SEQUENCING_ACCURACY=20 PRIMER_WT_END_QUAL=0.0 PRIMER_INTERNAL_WT_END_QUAL=0.0 = primer3-2.3.6/primer3web_v4_0_0_default_settings.txt0000664000077300007730000001020612231156245021370 0ustar ioanaioanaPrimer3 File - http://primer3.sourceforge.net P3_FILE_TYPE=settings PRIMER_FIRST_BASE_INDEX=1 PRIMER_TASK=generic P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers PRIMER_MIN_THREE_PRIME_DISTANCE=3 PRIMER_EXPLAIN_FLAG=1 # Put something reasonable for PRIMER_MISPRIMING_LIBRARY depending # on the species you are designing primers for. PRIMER_MISPRIMING_LIBRARY= PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00 PRIMER_PRODUCT_SIZE_RANGE=150-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 # PRIMER_MAX_SELF_ANY_TH=45.00 PRIMER_MAX_SELF_END_TH=35.00 PRIMER_PAIR_MAX_COMPL_ANY_TH=45.00 PRIMER_PAIR_MAX_COMPL_END_TH=35.00 PRIMER_MAX_HAIRPIN_TH=24.00 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=40.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=70.00 PRIMER_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_MAX_SIZE=23 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=59.0 PRIMER_MAX_TM=62.0 PRIMER_PAIR_MAX_DIFF_TM=5.0 # PRIMER_TM_FORMULA=1 means use the SantaLucia parameters (Proc Natl Acad Sci 95:1460-65) PRIMER_TM_FORMULA=1 PRIMER_SALT_MONOVALENT=50.0 # PRIMER_SALT_CORRECTIONS=1 means use the salt correction in SantaLucia et al 1998 PRIMER_SALT_CORRECTIONS=1 # Millimolar conc of MgCl+2 PRIMER_SALT_DIVALENT=1.5 # Millimolar conc of dNTPs PRIMER_DNTP_CONC=0.6 PRIMER_DNA_CONC=50.0 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0 PRIMER_LOWERCASE_MASKING=0 # PRIMER_MIN_GC=30.0 PRIMER_MAX_GC=70.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_POLY_X=4 PRIMER_OUTSIDE_PENALTY=0 PRIMER_GC_CLAMP=0 PRIMER_LIBERAL_BASE=1 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_PICK_ANYWAY=1 PRIMER_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_WT_SELF_END_TH=0.0 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_WT_NUM_NS=0.0 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_WT_END_QUAL=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_END_TH=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_END_QUAL=0.0 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_PRODUCT_MAX_TM=1000000.0 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_SEQUENCING_ACCURACY=20 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MAX_SELF_END=3.00 PRIMER_QUALITY_RANGE_MAX=100 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_SEQUENCING_INTERVAL=250 PRIMER_INTERNAL_SALT_DIVALENT=1.5 PRIMER_MAX_SELF_ANY=8.00 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_SEQUENCING_SPACING=500 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_MIN_END_QUALITY=0 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_SEQUENCING_LEAD=50 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_MAX_END_GC=5 PRIMER_MIN_QUALITY=0 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_WT_SELF_ANY=0.0 PRIMER_MAX_TEMPLATE_MISPRIMING=12.00 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_SELF_END=0.0 PRIMER_PRODUCT_OPT_SIZE=0 PRIMER_PRODUCT_OPT_TM=0.0 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=24.00 PRIMER_INSIDE_PENALTY=-1.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_PRODUCT_MIN_TM=-1000000.0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_WT_SELF_END=0.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_TM_LT=1.0 = primer3-2.3.6/COPYING.txt0000664000077300007730000004310312231156245013730 0ustar ioanaioana GNU GENERAL PUBLIC LICENSE Version 2, June 1991 Copyright (C) 1989, 1991 Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed. Preamble The licenses for most software are designed to take away your freedom to share and change it. By contrast, the GNU General Public License is intended to guarantee your freedom to share and change free software--to make sure the software is free for all its users. This General Public License applies to most of the Free Software Foundation's software and to any other program whose authors commit to using it. (Some other Free Software Foundation software is covered by the GNU Lesser General Public License instead.) You can apply it to your programs, too. When we speak of free software, we are referring to freedom, not price. Our General Public Licenses are designed to make sure that you have the freedom to distribute copies of free software (and charge for this service if you wish), that you receive source code or can get it if you want it, that you can change the software or use pieces of it in new free programs; and that you know you can do these things. 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If the software is modified by someone else and passed on, we want its recipients to know that what they have is not the original, so that any problems introduced by others will not reflect on the original authors' reputations. Finally, any free program is threatened constantly by software patents. We wish to avoid the danger that redistributors of a free program will individually obtain patent licenses, in effect making the program proprietary. To prevent this, we have made it clear that any patent must be licensed for everyone's free use or not licensed at all. The precise terms and conditions for copying, distribution and modification follow. GNU GENERAL PUBLIC LICENSE TERMS AND CONDITIONS FOR COPYING, DISTRIBUTION AND MODIFICATION 0. This License applies to any program or other work which contains a notice placed by the copyright holder saying it may be distributed under the terms of this General Public License. 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You may copy and distribute verbatim copies of the Program's source code as you receive it, in any medium, provided that you conspicuously and appropriately publish on each copy an appropriate copyright notice and disclaimer of warranty; keep intact all the notices that refer to this License and to the absence of any warranty; and give any other recipients of the Program a copy of this License along with the Program. You may charge a fee for the physical act of transferring a copy, and you may at your option offer warranty protection in exchange for a fee. 2. You may modify your copy or copies of the Program or any portion of it, thus forming a work based on the Program, and copy and distribute such modifications or work under the terms of Section 1 above, provided that you also meet all of these conditions: a) You must cause the modified files to carry prominent notices stating that you changed the files and the date of any change. b) You must cause any work that you distribute or publish, that in whole or in part contains or is derived from the Program or any part thereof, to be licensed as a whole at no charge to all third parties under the terms of this License. c) If the modified program normally reads commands interactively when run, you must cause it, when started running for such interactive use in the most ordinary way, to print or display an announcement including an appropriate copyright notice and a notice that there is no warranty (or else, saying that you provide a warranty) and that users may redistribute the program under these conditions, and telling the user how to view a copy of this License. (Exception: if the Program itself is interactive but does not normally print such an announcement, your work based on the Program is not required to print an announcement.) These requirements apply to the modified work as a whole. If identifiable sections of that work are not derived from the Program, and can be reasonably considered independent and separate works in themselves, then this License, and its terms, do not apply to those sections when you distribute them as separate works. But when you distribute the same sections as part of a whole which is a work based on the Program, the distribution of the whole must be on the terms of this License, whose permissions for other licensees extend to the entire whole, and thus to each and every part regardless of who wrote it. Thus, it is not the intent of this section to claim rights or contest your rights to work written entirely by you; rather, the intent is to exercise the right to control the distribution of derivative or collective works based on the Program. In addition, mere aggregation of another work not based on the Program with the Program (or with a work based on the Program) on a volume of a storage or distribution medium does not bring the other work under the scope of this License. 3. You may copy and distribute the Program (or a work based on it, under Section 2) in object code or executable form under the terms of Sections 1 and 2 above provided that you also do one of the following: a) Accompany it with the complete corresponding machine-readable source code, which must be distributed under the terms of Sections 1 and 2 above on a medium customarily used for software interchange; or, b) Accompany it with a written offer, valid for at least three years, to give any third party, for a charge no more than your cost of physically performing source distribution, a complete machine-readable copy of the corresponding source code, to be distributed under the terms of Sections 1 and 2 above on a medium customarily used for software interchange; or, c) Accompany it with the information you received as to the offer to distribute corresponding source code. (This alternative is allowed only for noncommercial distribution and only if you received the program in object code or executable form with such an offer, in accord with Subsection b above.) The source code for a work means the preferred form of the work for making modifications to it. For an executable work, complete source code means all the source code for all modules it contains, plus any associated interface definition files, plus the scripts used to control compilation and installation of the executable. However, as a special exception, the source code distributed need not include anything that is normally distributed (in either source or binary form) with the major components (compiler, kernel, and so on) of the operating system on which the executable runs, unless that component itself accompanies the executable. If distribution of executable or object code is made by offering access to copy from a designated place, then offering equivalent access to copy the source code from the same place counts as distribution of the source code, even though third parties are not compelled to copy the source along with the object code. 4. You may not copy, modify, sublicense, or distribute the Program except as expressly provided under this License. Any attempt otherwise to copy, modify, sublicense or distribute the Program is void, and will automatically terminate your rights under this License. However, parties who have received copies, or rights, from you under this License will not have their licenses terminated so long as such parties remain in full compliance. 5. You are not required to accept this License, since you have not signed it. However, nothing else grants you permission to modify or distribute the Program or its derivative works. These actions are prohibited by law if you do not accept this License. Therefore, by modifying or distributing the Program (or any work based on the Program), you indicate your acceptance of this License to do so, and all its terms and conditions for copying, distributing or modifying the Program or works based on it. 6. Each time you redistribute the Program (or any work based on the Program), the recipient automatically receives a license from the original licensor to copy, distribute or modify the Program subject to these terms and conditions. You may not impose any further restrictions on the recipients' exercise of the rights granted herein. You are not responsible for enforcing compliance by third parties to this License. 7. If, as a consequence of a court judgment or allegation of patent infringement or for any other reason (not limited to patent issues), conditions are imposed on you (whether by court order, agreement or otherwise) that contradict the conditions of this License, they do not excuse you from the conditions of this License. If you cannot distribute so as to satisfy simultaneously your obligations under this License and any other pertinent obligations, then as a consequence you may not distribute the Program at all. For example, if a patent license would not permit royalty-free redistribution of the Program by all those who receive copies directly or indirectly through you, then the only way you could satisfy both it and this License would be to refrain entirely from distribution of the Program. If any portion of this section is held invalid or unenforceable under any particular circumstance, the balance of the section is intended to apply and the section as a whole is intended to apply in other circumstances. It is not the purpose of this section to induce you to infringe any patents or other property right claims or to contest validity of any such claims; this section has the sole purpose of protecting the integrity of the free software distribution system, which is implemented by public license practices. Many people have made generous contributions to the wide range of software distributed through that system in reliance on consistent application of that system; it is up to the author/donor to decide if he or she is willing to distribute software through any other system and a licensee cannot impose that choice. This section is intended to make thoroughly clear what is believed to be a consequence of the rest of this License. 8. If the distribution and/or use of the Program is restricted in certain countries either by patents or by copyrighted interfaces, the original copyright holder who places the Program under this License may add an explicit geographical distribution limitation excluding those countries, so that distribution is permitted only in or among countries not thus excluded. In such case, this License incorporates the limitation as if written in the body of this License. 9. The Free Software Foundation may publish revised and/or new versions of the General Public License from time to time. Such new versions will be similar in spirit to the present version, but may differ in detail to address new problems or concerns. Each version is given a distinguishing version number. If the Program specifies a version number of this License which applies to it and "any later version", you have the option of following the terms and conditions either of that version or of any later version published by the Free Software Foundation. If the Program does not specify a version number of this License, you may choose any version ever published by the Free Software Foundation. 10. If you wish to incorporate parts of the Program into other free programs whose distribution conditions are different, write to the author to ask for permission. For software which is copyrighted by the Free Software Foundation, write to the Free Software Foundation; we sometimes make exceptions for this. Our decision will be guided by the two goals of preserving the free status of all derivatives of our free software and of promoting the sharing and reuse of software generally. NO WARRANTY 11. BECAUSE THE PROGRAM IS LICENSED FREE OF CHARGE, THERE IS NO WARRANTY FOR THE PROGRAM, TO THE EXTENT PERMITTED BY APPLICABLE LAW. EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT HOLDERS AND/OR OTHER PARTIES PROVIDE THE PROGRAM "AS IS" WITHOUT WARRANTY OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM IS WITH YOU. SHOULD THE PROGRAM PROVE DEFECTIVE, YOU ASSUME THE COST OF ALL NECESSARY SERVICING, REPAIR OR CORRECTION. 12. IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING WILL ANY COPYRIGHT HOLDER, OR ANY OTHER PARTY WHO MAY MODIFY AND/OR REDISTRIBUTE THE PROGRAM AS PERMITTED ABOVE, BE LIABLE TO YOU FOR DAMAGES, INCLUDING ANY GENERAL, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE USE OR INABILITY TO USE THE PROGRAM (INCLUDING BUT NOT LIMITED TO LOSS OF DATA OR DATA BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS), EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. END OF TERMS AND CONDITIONS How to Apply These Terms to Your New Programs If you develop a new program, and you want it to be of the greatest possible use to the public, the best way to achieve this is to make it free software which everyone can redistribute and change under these terms. To do so, attach the following notices to the program. It is safest to attach them to the start of each source file to most effectively convey the exclusion of warranty; and each file should have at least the "copyright" line and a pointer to where the full notice is found. Copyright (C) This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA. Also add information on how to contact you by electronic and paper mail. If the program is interactive, make it output a short notice like this when it starts in an interactive mode: Gnomovision version 69, Copyright (C) year name of author Gnomovision comes with ABSOLUTELY NO WARRANTY; for details type `show w'. This is free software, and you are welcome to redistribute it under certain conditions; type `show c' for details. The hypothetical commands `show w' and `show c' should show the appropriate parts of the General Public License. Of course, the commands you use may be called something other than `show w' and `show c'; they could even be mouse-clicks or menu items--whatever suits your program. You should also get your employer (if you work as a programmer) or your school, if any, to sign a "copyright disclaimer" for the program, if necessary. Here is a sample; alter the names: Yoyodyne, Inc., hereby disclaims all copyright interest in the program `Gnomovision' (which makes passes at compilers) written by James Hacker. , 1 April 1989 Ty Coon, President of Vice This General Public License does not permit incorporating your program into proprietary programs. If your program is a subroutine library, you may consider it more useful to permit linking proprietary applications with the library. If this is what you want to do, use the GNU Lesser General Public License instead of this License. primer3-2.3.6/src/0000775000077300007730000000000012231156365012650 5ustar ioanaioanaprimer3-2.3.6/src/primer3_boulder_main.c0000664000077300007730000004426212231156245017122 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009, 2010,2011,2012 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of primer3 and the libprimer3 library. Primer3 and the libprimer3 library are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this software (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include #include #include /* strcmp() */ #include /* free() */ #include /* getopt() */ #include #include #include #include "thal.h" #include "format_output.h" #include "libprimer3.h" #include "read_boulder.h" #include "print_boulder.h" /* Check on which OS we compile */ #if defined(_WIN32) || defined(WIN32) || defined (__WIN32__) || defined(__CYGWIN__) || defined(__MINGW32__) #define OS_WIN #endif /* Some function prototypes */ static void print_usage(); static void sig_handler(int); static void read_thermodynamic_parameters(); /* Other global variables. */ static const char *pr_release; static const char *pr_program_name; int main(int argc, char *argv[]) { /* Setup the input data structures handlers */ int format_output = 0; int strict_tags = 0; int echo_settings = 0; int io_version = 4; int default_version = 2; int dump_args = 0; p3_global_settings *global_pa; seq_args *sarg; read_boulder_record_results read_boulder_record_res = {0,0}; pr_append_str p3_settings_path; pr_append_str output_path; pr_append_str error_path; pr_append_str fatal_parse_err; pr_append_str nonfatal_parse_err; pr_append_str warnings; /* Some variables needed by getopt */ int opt, option_index = 0; struct option long_options[] = { {"about", no_argument, 0, 'a'}, {"format_output", no_argument, &format_output, 1}, {"strict_tags", no_argument, &strict_tags, 1}, {"p3_settings_file", required_argument, 0, 'p'}, {"echo_settings_file", no_argument, &echo_settings, 1}, {"io_version", required_argument, 0, 'i'}, {"default_version", required_argument, 0, 'd'}, {"Dump_args", no_argument, 0, 'D'}, {"2x_compat", no_argument, 0, '2'}, {"output", required_argument, 0, 'o'}, {"error", required_argument, 0, 'e'}, {0, 0, 0, 0} }; int about = 0, compat = 0, invalid_flag = 0; /* Retval will point to the return value from choose_primers(). */ p3retval *retval = NULL; int input_found=0; init_pr_append_str(&fatal_parse_err); init_pr_append_str(&nonfatal_parse_err); init_pr_append_str(&warnings); init_pr_append_str(&p3_settings_path); init_pr_append_str(&output_path); init_pr_append_str(&error_path); /* Get the program name for correct error messages */ pr_release = libprimer3_release(); pr_program_name = argv[0]; p3_set_program_name(pr_program_name); /* We set up some signal handlers in case someone starts up the program * from the command line, wonders why nothing is happening, and then kills * the program. */ signal(SIGINT, sig_handler); signal(SIGTERM, sig_handler); /* Read in the flags provided with the program call */ opterr = 0; while ((opt = getopt_long_only(argc, argv, "", long_options, &option_index)) != -1) { switch (opt) { case 'a': about = 1; break; case 'p': if (pr_append_external(&p3_settings_path, optarg)) { exit(-2); /* Out of memory. */ } break; case 'i': if (!strcmp(optarg, "4")) io_version = 4; else io_version = -1; break; case 'd': /* default_version */ if (!strcmp(optarg, "1")) default_version = 1; else if (!strcmp(optarg, "2")) default_version = 2; else default_version = -1; break; case 'D': /* Undocumented flag for testing; causes values of arguments to be echoed to stdout. */ dump_args = 1; break; case '2': compat = 1; break; case 'o': if (pr_append_external(&output_path, optarg)) { exit(-2); /* Out of memory. */ } break; case 'e': if (pr_append_external(&error_path, optarg)) { exit(-2); /* Out of memory. */ } break; case '?': invalid_flag = 1; break; } } /* Open the output and error files specified */ if (!pr_is_empty(&error_path)) { /* reassign stderr */ if (freopen(pr_append_str_chars(&error_path), "w", stderr) == NULL) { fprintf(stderr, "Error creating file %s\n", pr_append_str_chars(&error_path)); exit(-1); } destroy_pr_append_str_data(&error_path); } if (!pr_is_empty(&output_path)) { /* reassign stdout */ if (freopen(pr_append_str_chars(&output_path), "w", stdout) == NULL) { fprintf(stderr, "Error creating file %s\n", pr_append_str_chars(&output_path)); exit(-1); } destroy_pr_append_str_data(&output_path); } /* We do any printing after redirecting stdout and stderr */ if (about == 1) { printf("%s\n", pr_release); exit(0); } if ((io_version == -1) || (invalid_flag == 1) || (default_version == -1)) { print_usage(); exit(-1); } if (compat == 1) { printf("PRIMER_ERROR=flag -2x_compat is no longer supported\n=\n"); exit(-1); } /* Check if an input file has been specified on the command line. */ if (optind < argc) { if (optind + 1 != argc) { print_usage(); exit(-1); } if (freopen(argv[optind], "r", stdin) == NULL) { fprintf(stderr, "Error opening file %s\n", argv[optind]); exit(-1); } } /* Allocate the space for global settings and fill in default parameters */ if (default_version == 1) global_pa = p3_create_global_settings_default_version_1(); else if (default_version == 2) global_pa = p3_create_global_settings(); else { print_usage(); exit(-1); } if (!global_pa) { exit(-2); /* Out of memory. */ } global_pa->dump = dump_args ; /* Settings files have to be read in just below, and the functions need a temporary sarg */ if (!(sarg = create_seq_arg())) { exit(-2); } /* Read data from the settings file until a "=" line occurs. Assign parameter values for primer picking to pa and sa. */ if (!pr_is_empty(&p3_settings_path)) { read_p3_file(pr_append_str_chars(&p3_settings_path), settings, echo_settings && !format_output, strict_tags, global_pa, sarg, &fatal_parse_err, &nonfatal_parse_err, &warnings, &read_boulder_record_res); destroy_pr_append_str_data(&p3_settings_path); /* Check if any thermodynamical alignment flag was given */ if ((global_pa->thermodynamic_oligo_alignment == 1) || (global_pa->thermodynamic_template_alignment == 1)) read_thermodynamic_parameters(); } /* We also need to print out errors here because the loop erases all errors at start. If there are fatal errors, write the proper message and exit */ if (fatal_parse_err.data != NULL) { if (format_output) { format_error(stdout, sarg->sequence_name, fatal_parse_err.data); } else { print_boulder_error(fatal_parse_err.data); } fprintf(stderr, "%s: %s\n", pr_program_name, fatal_parse_err.data); destroy_seq_args(sarg); exit(-4); } /* If there are nonfatal errors, write the proper message * and skip to the end of the loop */ if (!pr_is_empty(&nonfatal_parse_err)) { if (format_output) { format_error(stdout, sarg->sequence_name, nonfatal_parse_err.data); } else { print_boulder_error(nonfatal_parse_err.data); } } /* The temporary sarg is not needed any more */ destroy_seq_args(sarg); sarg = NULL; /* Read the data from input stream record by record and process it if * there are no errors. This is where the work is done. */ while (1) { /* Create and initialize a seq_args data structure. sa (seq_args *) is * initialized here because Values are _not_ retained across different * input records. */ if (!(sarg = create_seq_arg())) { exit(-2); } /* Reset all errors handlers and the return structure */ pr_set_empty(&fatal_parse_err); pr_set_empty(&nonfatal_parse_err); pr_set_empty(&warnings); retval = NULL; /* See read_boulder.h for documentation on read_boulder_record().*/ if (!read_boulder_record(stdin, &strict_tags, &io_version, !format_output, all_parameters, global_pa, sarg, &fatal_parse_err, &nonfatal_parse_err, &warnings, &read_boulder_record_res)) { break; /* There were no more boulder records */ } /* Check if any thermodynamical alignment flag was given and the path to the parameter files changed - we need to reread them */ if (((global_pa->thermodynamic_oligo_alignment == 1) || (global_pa->thermodynamic_template_alignment == 1)) && (thermodynamic_path_changed == 1)) read_thermodynamic_parameters(); /* Check that we found the thermodynamic parameters in case any thermodynamic flag was set to 1. */ if (((global_pa->thermodynamic_oligo_alignment == 1) || (global_pa->thermodynamic_template_alignment == 1)) && (thermodynamic_params_path == NULL)) { /* no parameter directory found, error */ printf("PRIMER_ERROR=thermodynamic approach chosen, but path to thermodynamic parameters not specified\n=\n"); exit(-1); } input_found = 1; if ((global_pa->primer_task == generic) && (global_pa->pick_internal_oligo == 1)){ PR_ASSERT(global_pa->pick_internal_oligo); } /* If there are fatal errors, write the proper message and exit */ if (fatal_parse_err.data != NULL) { if (format_output) { format_error(stdout, sarg->sequence_name, fatal_parse_err.data); } else { print_boulder_error(fatal_parse_err.data); } fprintf(stderr, "%s: %s\n", pr_program_name, fatal_parse_err.data); destroy_p3retval(retval); destroy_seq_args(sarg); exit(-4); } /* If there are nonfatal errors, write the proper message * and skip to the end of the loop */ if (!pr_is_empty(&nonfatal_parse_err)) { if (format_output) { format_error(stdout, sarg->sequence_name, nonfatal_parse_err.data); } else { print_boulder_error(nonfatal_parse_err.data); } goto loop_wrap_up; } /* Print any warnings and continue processing */ if (!pr_is_empty(&warnings)) { if (format_output) { format_warning(stdout, sarg->sequence_name, warnings.data); } else { print_boulder_warning(warnings.data); } } if (read_boulder_record_res.file_flag && sarg->sequence_name == NULL) { /* We will not have a base name for the files */ if (format_output) { format_error(stdout, NULL, "Need PRIMER_SEQUENCE_ID if PRIMER_FILE_FLAG is not 0"); } else { print_boulder_error("Need PRIMER_SEQUENCE_ID if PRIMER_FILE_FLAG is not 0"); } goto loop_wrap_up; } /* Pick the primers - the central function */ p3_set_gs_primer_file_flag(global_pa, read_boulder_record_res.file_flag); retval = choose_primers(global_pa, sarg); if (NULL == retval) exit(-2); /* Out of memory. */ /* If it was necessary to use a left_input, right_input, or internal_oligo_input primer that was unacceptable, then add warnings. */ if (global_pa->pick_anyway && format_output) { if (sarg->left_input) { add_must_use_warnings(&retval->warnings, "Left primer", &retval->fwd.expl); } if (sarg->right_input) { add_must_use_warnings(&retval->warnings, "Right primer", &retval->rev.expl); } if (sarg->internal_input) { add_must_use_warnings(&retval->warnings, "Hybridization probe", &retval->intl.expl); } } if (pr_is_empty(&retval->glob_err) && pr_is_empty(&retval->per_sequence_err)) { /* We need to test for errors before we call p3_print_oligo_lists. This function only works on retval as returned when there were no errors. */ if (read_boulder_record_res.file_flag) { /* Create files with left, right, and internal oligos. */ p3_print_oligo_lists(retval, sarg, global_pa, &retval->per_sequence_err, sarg->sequence_name); } } if (format_output) { print_format_output(stdout, &io_version, global_pa, sarg, retval, pr_release, read_boulder_record_res.explain_flag); } else { /* Use boulder output */ print_boulder(io_version, global_pa, sarg, retval, read_boulder_record_res.explain_flag); } loop_wrap_up: /* Here the failed loops join in again */ if (NULL != retval) { /* Check for errors and print them */ if (NULL != retval->glob_err.data) { fprintf(stderr, "%s: %s\n", pr_program_name, retval->glob_err.data); destroy_p3retval(retval); destroy_seq_args(sarg); exit(-4); } } destroy_p3retval(retval); /* This works even if retval is NULL */ retval = NULL; destroy_seq_args(sarg); sarg = NULL; } /* while (1) (processing boulder io records) ... End of the primary working loop */ /* To avoid being distracted when looking for leaks: */ if ((global_pa->thermodynamic_oligo_alignment == 1) || (global_pa->thermodynamic_template_alignment == 1)) destroy_thal_structures(); p3_destroy_global_settings(global_pa); global_pa = NULL; destroy_seq_args(sarg); destroy_pr_append_str_data(&nonfatal_parse_err); destroy_pr_append_str_data(&fatal_parse_err); destroy_pr_append_str_data(&warnings); destroy_dpal_thal_arg_holder(); free(thermodynamic_params_path); /* If it could not read input, then complain and die */ if (0 == input_found) { print_usage(); exit(-3); } return 0; } /* Reads the thermodynamic parameters if the thermodynamic alignment tag was set to 1 */ static void read_thermodynamic_parameters() { thal_results o; /* if the path to the parameter files did not change, we do not want to read again */ if (thermodynamic_path_changed == 0) return; /* check that the path to the parameters folder was given */ if (thermodynamic_params_path == NULL) { #ifdef OS_WIN /* in windows check for .\\primer3_config */ struct stat st; if ((stat(".\\primer3_config", &st) == 0) && S_ISDIR(st.st_mode)) { thermodynamic_params_path = (char*) malloc(strlen(".\\primer3_config\\") * sizeof(char) + 1); if (NULL == thermodynamic_params_path) exit (-2); /* Out of memory */ strcpy(thermodynamic_params_path, ".\\primer3_config\\"); } else { /* no default directory found */ return; } #else /* in linux, check for ./primer3_config and /opt/primer3_config */ struct stat st; if ((stat("./primer3_config", &st) == 0) && S_ISDIR(st.st_mode)) { thermodynamic_params_path = (char*) malloc(strlen("./primer3_config/") * sizeof(char) + 1); if (NULL == thermodynamic_params_path) exit (-2); /* Out of memory */ strcpy(thermodynamic_params_path, "./primer3_config/"); } else if ((stat("/opt/primer3_config", &st) == 0) && S_ISDIR(st.st_mode)) { thermodynamic_params_path = (char*) malloc(strlen("/opt/primer3_config/") * sizeof(char) + 1); if (NULL == thermodynamic_params_path) exit (-2); /* Out of memory */ strcpy(thermodynamic_params_path, "/opt/primer3_config/"); } else { /* no default directory found */ return; } #endif } /* read in the thermodynamic parameters */ if (get_thermodynamic_values(thermodynamic_params_path, &o)) { fprintf(stderr, "%s\n", o.msg); exit(-1); } /* mark that the last given path was used for reading the parameters */ thermodynamic_path_changed = 0; } /* Print out copyright and a short usage message*/ static void print_usage() { fprintf(stderr, "%s", primer3_copyright()); fprintf(stderr, "\n\nUSAGE: %s %s %s %s %s %s %s %s %s %s\n", pr_program_name, "[-format_output]", "[-default_version=1|-default_version=2]", "[-io_version=4]", "[-p3_settings_file=]", "[-echo_settings_file]", "[-strict_tags]", "[-output=]", "[-error=]", "[input_file]"); fprintf(stderr, "This is primer3 (%s)\n", pr_release); fprintf(stderr, "Input can also be provided on standard input.\n"); fprintf(stderr, "For example:\n"); fprintf(stderr, "$ primer3_core < my_input_file\n"); } /* Print out copyright, a short usage message and the signal */ static void sig_handler(int signal) { print_usage(); fprintf(stderr, "%s: received signal %d\n", pr_program_name, signal); exit(signal); } primer3-2.3.6/src/dpal.c0000664000077300007730000010676212231156245013745 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of primer3 software suite. This software suite is is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this software (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include #include #include #include #include #include "dpal.h" /* * We should probably remove the DPAL_FORGET_PATH compile-time option. * Efficiency now derives primarily from specialized versions of _dpal* for * particular parameter values. */ #ifndef DPAL_FORGET_PATH /* * Print an alignment on stderr, given the 2 aligned sequences, the "trace" * matrix, and the coordinate of the end point of the alignment to print. */ static void print_align(const unsigned char *, const unsigned char *, int[DPAL_MAX_ALIGN][DPAL_MAX_ALIGN][3], int, int, const dpal_args*); #endif /* * Return 1 if there is an illegal character in the first argument, and place * the illegal character in the address contained in the last argument. */ static int illegal_char(const unsigned char *, const dpal_ssm, char *); static const unsigned char *xlate_ambiguity_code(char); /* * The next function headers are for various versions of the * dynamic-programming alignment code optimized for particular input argument * values. */ static void _dpal_generic(const unsigned char *, const unsigned char *, const int, const int, const dpal_args *, dpal_results *); static void _dpal_long_nopath_maxgap1_local_end(const unsigned char *, const unsigned char *, const int, const int, const dpal_args *, dpal_results *); static void _dpal_long_nopath_generic(const unsigned char *, const unsigned char *, const int, const int, const dpal_args *, dpal_results *); static void _dpal_long_nopath_maxgap1_local(const unsigned char *, const unsigned char *, const int, const int, const dpal_args *, dpal_results *); static void _dpal_long_nopath_maxgap1_global_end(const unsigned char *, const unsigned char *, const int, const int, const dpal_args *, dpal_results *); /* Set dpal args to appropriate values for primer picking. */ /* IMPORTANT -- if the scoring system changes, then the value returned by align() for short target sequences must be adjusted. */ void set_dpal_args(dpal_args *a) { unsigned int i, j; memset(a, 0, sizeof(*a)); for (i = 0; i <= UCHAR_MAX; i++) for (j = 0; j <= UCHAR_MAX; j++) if (('A' == i || 'C' == i || 'G' == i || 'T' == i || 'N' == i) && ('A' == j || 'C' == j || 'G' == j || 'T' == j || 'N' == j)) { if (i == 'N' || j == 'N') a->ssm[i][j] = -25; else if (i == j) a->ssm[i][j] = 100; else a->ssm[i][j] = -100; } else a->ssm[i][j] = INT_MIN; a->gap = -200; a->gapl = -200; a->flag = DPAL_LOCAL; a->max_gap = 1; a->fail_stop = 1; a->check_chars = 0; a->debug = 0; a->score_only = 1; a->force_generic = 0; a->force_long_generic = 0; a->force_long_maxgap1 = 0; } void dpal_set_default_nt_args(dpal_args *a) { set_dpal_args(a); a->check_chars = 1; a->debug = 0; a->fail_stop = DPAL_EXIT_ON_ERROR; a->gap = -100; a->gapl = -100; a->max_gap = 3; a->score_only = 0; } void dpal_set_h_nt_matrix(dpal_args *a) { unsigned int i, j; for (i = 0; i <= UCHAR_MAX; i++) for (j = 0; j <= UCHAR_MAX; j++) if (('A' == i || 'C' == i || 'G' == i || 'T' == i || 'N' == i) && ('A' == j || 'C' == j || 'G' == j || 'T' == j || 'N' == j)) { if (i == 'N' || j == 'N') a->ssm[i][j] = -50; else if (i == j) { if ('C' == i || 'G' == i) a->ssm[i][j] = 300; else a->ssm[i][j] = 200; } else a->ssm[i][j] = -50; } else a->ssm[i][j] = INT_MIN; } /* The argument a must be a DNA scoring matrix. Modify a so that it for a match between any two ambiguity codes (or between ambiguity code and base), e.g. B and S, the score will be the maximum of score between any base in B and any base in S, in the example between any pair in {C, G, T} X {C, G}. */ int dpal_set_ambiguity_code_matrix(dpal_args *a) { const unsigned char *c1, *c2; const unsigned char *amb_codes = (unsigned char *) "BDHVRYKMSWN"; const unsigned char *all_bases = (unsigned char *) "ACGT"; const unsigned char *bases1, *bases2, *b1, *b2; int extreme; for (c1 = amb_codes; *c1; c1++) { bases1 = xlate_ambiguity_code(*c1); if (!bases1) return 0; /* Do matches between c1 and all other ambiguity codes. */ for (c2 = amb_codes; *c2; c2++) { bases2 = xlate_ambiguity_code(*c2); if (!bases2) return 0; extreme = INT_MIN; for (b1 = bases1; *b1; b1++) { for (b2 = bases2; *b2; b2++) { if (a->ssm[*b1][*b2] > extreme) { extreme = a->ssm[*b1][*b2]; } } } /* extreme is now the maximum score for a match between any 2 bases represented *c1, *c2. */ a->ssm[*c1][*c2] = extreme; } /* Do matches between c1 and all bases. */ for (b2 = all_bases; *b2; b2++) { extreme = INT_MIN; for (b1 = bases1; *b1; b1++) { if (a->ssm[*b1][*b2] > extreme) { extreme = a->ssm[*b1][*b2]; } } a->ssm[*c1][*b2] = extreme; a->ssm[*b2][*c1] = extreme; } } return 1; } static const unsigned char * xlate_ambiguity_code(char c) { if ('N' == c) return (unsigned char *) "ACGT"; else if ('B' == c) return (unsigned char *) "CGT"; else if ('D' == c) return (unsigned char *) "AGT"; else if ('H' == c) return (unsigned char*) "ACT"; else if ('V' == c) return (unsigned char*) "ACG"; else if ('R' == c) return (unsigned char*) "AG"; else if ('Y' == c) return (unsigned char*) "CT"; else if ('K' == c) return (unsigned char*) "GT"; else if ('M' == c) return (unsigned char*) "AC"; else if ('S' == c) return (unsigned char*) "CG"; else if ('W' == c) return (unsigned char*) "AT"; else return NULL; /* Error condition */ } /* The next two macros require that the output argument is always called 'out'. */ #define CHECK_ERROR(COND,MSG) if (COND) { out->msg = MSG; goto FAIL; } /* If fail_stop is set, the code at FAIL: will use fprintf, otherwise will return with msg set. */ #define DPAL_OOM_ERROR {out->msg = "Out of memory", errno=ENOMEM; goto FAIL; } static void fail_action(const dpal_args *in, dpal_results *out) { if (in->fail_stop) { fprintf(stderr, "\n%s\n", out->msg); exit(-1); } out->score=DPAL_ERROR_SCORE; } void dpal(const unsigned char *X, const unsigned char *Y, const dpal_args *in, dpal_results *out) { int xlen, ylen; char msg[] = "Illegal character in input: ?"; out->score = DPAL_ERROR_SCORE; out->path_length = 0; out->msg = NULL; CHECK_ERROR(NULL == X, "NULL first sequence"); CHECK_ERROR(NULL == Y, "NULL second sequence"); CHECK_ERROR(NULL == in, "NULL 'in' pointer"); if (NULL == out) return; /* Leave it to the caller to crash */ CHECK_ERROR(in->flag != DPAL_GLOBAL && in->flag != DPAL_GLOBAL_END && in->flag != DPAL_LOCAL_END && in->flag != DPAL_LOCAL, "Illegal flag"); if (in->check_chars) { CHECK_ERROR(illegal_char(X, in->ssm, &msg[28]), msg); CHECK_ERROR(illegal_char(Y, in->ssm, &msg[28]), msg); } xlen = strlen((char *) X); ylen = strlen((char *) Y); out->align_end_1 = -1; out->align_end_2 = -1; if ('\0' == *X) { out->msg = "Empty first sequence"; out->score = 0; return; } if ('\0' == *Y) { out->msg = "Empty second sequence"; out->score = 0; return; } CHECK_ERROR(in->debug != 0 && in->score_only != 0, "score_only must be 0 if debug is non-0"); if (1 == in->force_generic || in->debug == 1 || 0 == in->score_only) { /* * A true value of in->debug really means "print alignment on stderr" * and implies 0 == a.score_only. */ CHECK_ERROR(xlen > DPAL_MAX_ALIGN, "Sequence 1 longer than DPAL_MAX_ALIGN and alignment is requested"); CHECK_ERROR(ylen > DPAL_MAX_ALIGN, "Sequence 2 longer than DPAL_MAX_ALIGN and alignment is requested"); _dpal_generic(X, Y, xlen, ylen, in, out); } else if (1 == in->force_long_generic) { _dpal_long_nopath_generic(X, Y, xlen, ylen, in, out); } else if (1 == in->max_gap ) { if (DPAL_LOCAL == in->flag) _dpal_long_nopath_maxgap1_local(X, Y, xlen, ylen, in, out); else if (DPAL_GLOBAL_END == in->flag) _dpal_long_nopath_maxgap1_global_end(X, Y, xlen, ylen, in, out); else if (DPAL_LOCAL_END == in->flag) _dpal_long_nopath_maxgap1_local_end(X, Y, xlen, ylen, in, out); else if (xlen <= DPAL_MAX_ALIGN && ylen <= DPAL_MAX_ALIGN) _dpal_generic(X, Y, xlen, ylen, in, out); else _dpal_long_nopath_generic(X, Y, xlen, ylen, in, out); } else if (xlen < DPAL_MAX_ALIGN && ylen < DPAL_MAX_ALIGN) _dpal_generic(X, Y, xlen, ylen, in, out); else _dpal_long_nopath_generic(X, Y, xlen, ylen, in, out); return; FAIL: fail_action(in, out); } static void _dpal_generic(const unsigned char *X, const unsigned char *Y, const int xlen, const int ylen, const dpal_args *in, dpal_results *out) { /* The "score matrix" (matrix of best scores). */ static int S[DPAL_MAX_ALIGN][DPAL_MAX_ALIGN]; #ifndef DPAL_FORGET_PATH /* The matrix of "trace" pointers */ static int P[DPAL_MAX_ALIGN][DPAL_MAX_ALIGN][3]; #endif register int i, j, k, mg, c; register int gap = in->gap, gapl = in->gapl, max_gap = in->max_gap; #ifndef DPAL_FORGET_PATH int i0 = -99, j0 = -99; int saved_k; #endif int I = -99, J = -99; /* Coordinates of the maximum score. */ int smax; /* The optimum score. */ int score = -99; /* Current score. */ int a,b,max; #ifdef DPAL_PRINT_COVERAGE fprintf(stderr, "_dpal_generic called\n"); #endif CHECK_ERROR(xlen > DPAL_MAX_ALIGN, "First sequence too long for _dpal_generic"); CHECK_ERROR(ylen > DPAL_MAX_ALIGN, "Second sequence too long for _dpal_generic"); /* Initialize the 0th column of the score matrix. */ smax = INT_MIN; for(i=0; i < xlen; i++) { score = in->ssm[X[i]][Y[0]]; if (DPAL_LOCAL == in->flag) { if (score < 0) score = 0; if(score > smax) { smax = score; I=i; J=0; } } else if (DPAL_LOCAL_END == in->flag) {if (score < 0) score = 0;} S[i][0] = score; } /* Move code for find global-alignment and end-anchored alignment below? */ if (DPAL_LOCAL != in->flag) { /* * For a non-local alignment we restrict our search for the maximum * score to the last row. */ smax = S[xlen-1][0]; I=xlen-1; J=0; } /* Initialize the 0th row of the score matrix. */ for(j=0; jssm[X[0]][Y[j]]; if(DPAL_LOCAL == in->flag){ if (score < 0) score = 0; if(score > smax){ smax = score; I=0; J=j; } } else if (DPAL_LOCAL_END == in->flag) {if (score < 0) score = 0;} S[0][j] = score; } if(DPAL_GLOBAL == in->flag&&S[0][ylen-1]>smax){ smax = S[0][ylen-1]; I=0; J=ylen-1; } /* Further is the solution for dynamic programming problem. */ for(i=1; i 1) { b = S[i-2][j-1] + gap; #ifndef DPAL_FORGET_PATH i0 = i - 2; #endif } if (j > 1) { c = S[i-1][j-2] + gap; #ifndef DPAL_FORGET_PATH j0 = j - 2; #endif } } else if (max_gap > 1) { max=INT_MIN; mg=(max_gap+1>i||max_gap<0)?i:max_gap+1; for(k=2; k<=mg; k++) { c = S[i-k][j-1] + gap + gapl*(k-2); if(c>max){ max=c; #ifndef DPAL_FORGET_PATH i0 = i-k; #endif } } b=max; max=INT_MIN; mg=(max_gap+1>j||max_gap<0)?j:max_gap+1; for(k=2;k<=mg;k++) { c = S[i-1][j-k] + gap + gapl*(k-2); if(c>max){ max=c; #ifndef DPAL_FORGET_PATH j0 = j-k; #endif } } c=max; } if(a>=b && a>=c) { score = a + in->ssm[X[i]][Y[j]]; #ifndef DPAL_FORGET_PATH P[i][j][1] = i-1; P[i][j][2] = j-1; #endif } else if (b > a && b >= c) { score = b + in->ssm[X[i]][Y[j]]; #ifndef DPAL_FORGET_PATH P[i][j][1] = i0; P[i][j][2] = j-1; #endif } else if (c > a && c > b) { score = c + in->ssm[X[i]][Y[j]]; #ifndef DPAL_FORGET_PATH P[i][j][1] = i-1; P[i][j][2] = j0; #endif } if (score >= smax) /* * Because of comparison '>=' immediately above, dpal reports * ungapped (i.e. diagonal) alignments if there is a choice * of more than one optimum alignment. */ /* Move code to get 'g' and 'e' maxima to a separate loop ? */ if (DPAL_LOCAL == in->flag || (DPAL_GLOBAL_END == in->flag && i == xlen-1) || (DPAL_LOCAL_END == in->flag && i == xlen-1) || (DPAL_GLOBAL == in->flag&& (i==xlen-1||j==ylen-1))) { /* * If in->flag is DPAL_LOCAL, then a cell anywhere within * S may be the endpoint of the alignment. If in->flag is * DPAL_GLOBAL_END, then only cells in the last row may be * the endpoint. If in->flag is DPAL_GLOBAL cells in the * last row _or_ the last column may be the endpoint. */ smax = score; I = i; J = j; } /* put else here ? */ if (score < 0 && (DPAL_LOCAL == in->flag || DPAL_LOCAL_END == in->flag)) /* * For a local alignment, 0 is the lowest score that we record * in S. */ score = 0; S[i][j]=score; } } /* I and J now specify the last pair of an optimum alignment. */ #ifndef DPAL_FORGET_PATH k = (I > J) ? I+1 : J+1; saved_k=k; out->path[k][0]=I; out->path[k][1]=J; while(out->path[k][0]!=0&&out->path[k][1]!=0) { if ((in->flag== DPAL_LOCAL || in->flag == DPAL_LOCAL_END) &&S[out->path[k][0]][out->path[k][1]]==0) { k++; break; } out->path[k-1][0] = P[out->path[k][0]][out->path[k][1]][1]; out->path[k-1][1] = P[out->path[k][0]][out->path[k][1]][2]; k--; } if (k>0) { for (i=0;i<=saved_k-k;i++) { out->path[i][0] = out->path[i+k][0]; out->path[i][1] = out->path[i+k][1]; } } #endif if ((DPAL_LOCAL == in->flag || DPAL_LOCAL_END == in->flag)&& S[I][J] <= 0) { /* There is no alignment at all. */ out->score = 0; out->path_length = 0; } else { out->score = smax; out->align_end_1 = I; out->align_end_2 = J; #ifndef DPAL_FORGET_PATH out->path_length = saved_k - k + 1; #else out->path_length = 0; #endif } #ifndef DPAL_FORGET_PATH if (in->debug) print_align(X,Y,P,I,J, in); #endif return; FAIL: fail_action(in, out); } /* _dpal_generic */ /* Linear space, no path, for any value of maxgap and for any alignment. */ static void _dpal_long_nopath_generic(const unsigned char *X, const unsigned char *Y, const int xlen, const int ylen, const dpal_args *in, dpal_results *out) { /* The "score matrix" (matrix of best scores). */ int **S, *SI, **P; register int i, j, k, mg, mgy, c; register int gap = in->gap, gapl = in->gapl, max_gap = in->max_gap; int I = -99, J = -99; /* Coordinates of the maximum score. */ int smax; /* The optimum score. */ int score; /* Current score. */ #ifdef DPAL_PRINT_COVERAGE fprintf(stderr, "_dpal_long_nopath_generic called\n"); #endif out->score = DPAL_ERROR_SCORE; out->path_length = 0; out->msg = NULL; P = (int **) malloc(sizeof(*S)*(max_gap+2)); if (!P) { DPAL_OOM_ERROR; } S = (int **) malloc(sizeof(*S)*(max_gap+2)); if (!S) { DPAL_OOM_ERROR; } for(i=0; issm[X[i]][Y[0]]; if (DPAL_LOCAL == in->flag) { if (score < 0) score = 0; if(score > smax) { smax = score; I=i; J=0; } } else if (DPAL_LOCAL_END == in->flag) {if (score < 0) score = 0;} S[0][i] = score; } /* Move code for find global-alignment and end-anchored alignment below? */ if (DPAL_LOCAL != in->flag) { /* * For a non-local alignment we restrict our search for the maximum * score to the last row. */ smax = S[0][xlen-1]; I=xlen-1; J=0; } /* Initialize the 0th row of the score matrix. for(j=0; jssm[X[0]][Y[j]]; if(DPAL_LOCAL == in->flag){ if (score < 0) score = 0; if(score > smax){ smax = score; I=0; J=j; } } S[0][j] = score; } if(DPAL_GLOBAL == in->flag&&S[0][ylen-1]>smax){ smax = S[0][ylen-1]; I=0; J=ylen-1; } */ /* Further is the solution for dynamic programming problem. */ for(j=1; jj||max_gap<0)?j:max_gap+1; score = in->ssm[X[0]][Y[j]]; if (DPAL_LOCAL == in->flag) { if (score < 0) score = 0; if(score > smax) smax = score; } else if (DPAL_LOCAL_END == in->flag) { if (score < 0) score = 0;} else if (DPAL_GLOBAL == in->flag && j == ylen-1 && score > smax) smax = score; S[mgy][0] = score; for(i=1; ii||max_gap<0)?i:max_gap+1; for(k=2; k<=mg; k++) if((c = S[mgy-1][i-k] + gap + gapl*(k-2)) > score)score = c; for(k=2;k<=mgy;k++) if((c = S[mgy-k][i-1] + gap + gapl*(k-2)) > score)score=c; score += in->ssm[X[i]][Y[j]]; if (score >= smax) /* * Because of comparison '>=' immediately above, dpal reports * ungapped (i.e. diagonal) alignments if there is a choice * of more than one optimum alignment. */ /* Move code to get 'g' and 'e' maxima to a separate loop ? */ if (DPAL_LOCAL == in->flag || ((DPAL_GLOBAL_END == in->flag || DPAL_LOCAL_END == in->flag) && i == xlen-1) || (DPAL_GLOBAL == in->flag&& (i==xlen-1||j==ylen-1))) { /* * If in->flag is DPAL_LOCAL, then a cell anywhere within * S may be the endpoint of the alignment. If in->flag is * DPAL_GLOBAL_END, then only cells in the last row may be * the endpoint. If in->flag is DPAL_GLOBAL cells in the * last row _or_ the last column may be the endpoint. */ smax = score; I = i; J = j; } /* put else here ? */ if (score < 0 && (DPAL_LOCAL == in->flag || DPAL_LOCAL_END == in->flag)) /* * For a local alignment, 0 is the lowest score that we record * in S. */ score = 0; S[mgy][i]=score; } if(mgy == max_gap + 1){ SI = S[0]; for(i=0; iflag && smax <= 0) { /* There is no alignment at all. */ out->score = 0; out->path_length = 0; } else { out->score = smax; out->align_end_1 = I; out->align_end_2 = J; } for(i=0; i< max_gap + 2; i++) free(P[i]); free(S); free(P); return; FAIL: fail_action(in, out); } /* _dpal_long_nopath_generic */ static void _dpal_long_nopath_maxgap1_local(const unsigned char *X, const unsigned char *Y, const int xlen, const int ylen, const dpal_args *in, dpal_results *out) { /* The "score matrix" (matrix of best scores). */ int *S0, *S1, *S2; int *P0, *P1, *P2; int *S; register int i, j; register int gap = in->gap; register int smax; /* The optimum score. */ register int score; /* Current score. */ register int a; #ifdef DPAL_PRINT_COVERAGE fprintf(stderr, "_dpal_long_nopath_maxgap1_local called\n"); #endif CHECK_ERROR(ylen < 3, "_dpal_long_nopath_maxgap1_local requires ylen >= 3\n"); /* Note: S2[0] and S2[1] do not get initialized in this case. */ P0 = (int *) malloc(sizeof(int)*ylen); if (!P0) { DPAL_OOM_ERROR; } P1 = (int *) malloc(sizeof(int)*ylen); if (!P1) { DPAL_OOM_ERROR; } P2 = (int *) malloc(sizeof(int)*ylen); if (!P2) { DPAL_OOM_ERROR; } S0 = P0; S1 = P1; S2 = P2; smax = 0; /* For local alignment score can never be less than 0. */ /* Initialize the 0th row of the score matrix. */ for(j=0; j < ylen; j++) { score = in->ssm[X[0]][Y[j]]; if (score < 0) score = 0; else if (score > smax) smax = score; /*S[0][j] = score;*/ S0[j] = score; } /* Set the 1st row of the score matrix. */ score = in->ssm[X[1]][Y[0]]; if(score < 0) score = 0; else if (score > smax) smax = score; S1[0] = score; for(j=1; j < ylen; j++) { score = S0[j-1]; if(j>1 && (a=S0[j-2] + gap) > score)score = a; score += in->ssm[X[1]][Y[j]]; if (score < 0) score = 0; else if(score > smax) smax = score; S1[j] = score; } for(i=2; i < xlen; i++) { score = in->ssm[X[i]][Y[0]]; if (score < 0) score = 0; else if (score > smax) smax = score; S2[0] = score; score = S1[0]; if((a=S0[0] + gap) > score) score = a; score += in->ssm[X[i]][Y[1]]; if(score < 0) score = 0; else if (score > smax) smax = score; S2[1] = score; for(j=2; j < ylen; j++) { score = S0[j-1]; if((a=S1[j-2])>score) score = a; score +=gap; if((a=S1[j-1]) >score) score = a; score += in->ssm[X[i]][Y[j]]; if (score < 0 ) score = 0; else if (score > smax) smax = score; S2[j]=score; } S = S0; S0 = S1; S1 = S2; S2 = S; } out->score = smax; out->path_length=0; free(P0); free(P1); free(P2); return; FAIL: fail_action(in, out); } /* _dpal_long_nopath_maxgap1_local */ static void _dpal_long_nopath_maxgap1_global_end(const unsigned char *X, const unsigned char *Y, const int xlen, const int ylen, const dpal_args *in, dpal_results *out) { /* The "score matrix" (matrix of best scores). */ int *S0, *S1, *S2, *S; int *P0, *P1, *P2; register int i, j, k; register int gap = in->gap; register int smax; /* The optimum score. */ register int score; /* Current score. */ register int a, t; #ifdef DPAL_PRINT_COVERAGE fprintf(stderr, "_dpal_long_nopath_maxgap1_global_end called\n"); #endif P0 = (int *) malloc(sizeof(int)*xlen); if (!P0) { DPAL_OOM_ERROR; } P1 = (int *) malloc(sizeof(int)*xlen); if (!P1) { DPAL_OOM_ERROR; } P2 = (int *) malloc(sizeof(int)*xlen); if (!P2) { DPAL_OOM_ERROR; } S0 = P0; S1 = P1; S2 = P2; smax = in->ssm[X[xlen-1]][Y[0]]; /* Set the 0th row of the score matrix. */ for(j=0; jssm[X[j]][Y[0]]; /* Set the 1st row of the score matrix. */ S1[0] = in->ssm[X[0]][Y[1]]; for(j=1; j < xlen; j++){ score = S0[j-1]; if(j>1 && (a=S0[j-2] + gap)> score)score = a; score += in->ssm[X[j]][Y[1]]; if(score > smax && j == xlen-1) smax = score; S1[j] = score; } k = ylen - (int)(xlen / 2) + 1; if (k<1) k = 1; /* Set the rectangular part of almost the remainder of the matrix. */ for(j=2; jssm[X[0]][Y[j]]; score = S1[0]; if((a=S0[0]+gap) > score) score = a; score += in->ssm[X[1]][Y[j]]; S2[1] = score; for(i=2; i score)score = a; score += gap; if((a=S1[i-1]) > score)score = a; score += in->ssm[X[i]][Y[j]]; S2[i] = score; } score = S1[xlen-3]; if((a=S0[xlen-2]) > score)score = a; score += gap; if((a=S1[xlen-2]) > score)score = a; score += in->ssm[X[xlen-1]][Y[j]]; S2[xlen-1] = score; if(score > smax) smax = score; S = S0; S0 = S1; S1 = S2; S2 = S; } /* Set the triangular part of almost the remainder of the matrix. */ t = 2; for(j=k+1; j score) score = a; score += gap; if((a=S1[i-1]) > score) score = a; score += in->ssm[X[i]][Y[j]]; S2[i] = score; } t += 2; score = S1[xlen-3]; if((a=S0[xlen-2]) > score)score = a; score += gap; if((a=S1[xlen-2]) > score)score = a; score += in->ssm[X[xlen-1]][Y[j]]; S2[xlen-1] = score; if(score > smax) smax = score; S = S0; S0 = S1; S1 = S2; S2 = S; } free(P0); free(P1); free(P2); out->score = smax; out->path_length=0; return; FAIL: fail_action(in, out); } /* _dpal_long_nopath_maxgap_global_end */ #ifndef DPAL_FORGET_PATH /* Reconstruct the best path and print the alignment. */ void print_align(const unsigned char *X, const unsigned char *Y, int P[DPAL_MAX_ALIGN][DPAL_MAX_ALIGN][3], int I, int J, const dpal_args *dargs) { int JX[DPAL_MAX_ALIGN],JY[DPAL_MAX_ALIGN]; int k,i,j,n,m; char sx[3*DPAL_MAX_ALIGN],sy[3*DPAL_MAX_ALIGN],sxy[3*DPAL_MAX_ALIGN]; for (i=0; i < 3*DPAL_MAX_ALIGN; i++) { sx[i] = ' '; sy[i] = ' '; sxy[i] = ' '; } if(I>J)k=I+1; else k=J+1; n=k; JX[k] = I; JY[k] = J; while(JX[k]!=0&&JY[k]!=0){ JX[k-1] = P[JX[k]][JY[k]][1]; JY[k-1] = P[JX[k]][JY[k]][2]; k--; } if(JX[k]>JY[k]){ for(i=0;issm[(unsigned char)sx[m]][(unsigned char)sy[m]] > 0) sxy[m] = '|'; else sxy[m]=' '; if(JX[i+1]-JX[i]>JY[i+1]-JY[i]){ for(j=1;jJX[i+1]-JX[i]){ for(j=1;jssm[(unsigned char)sx[m]][(unsigned char)sy[m]] > 0) sxy[m] = '|'; else sxy[m]=' '; m++; if (strlen((char *) X) - I > strlen((char *) Y)-J) { k = m + strlen((char *) X) - I; } else { k = m + strlen((char *) Y) - J; } j=0; while(jgap; register int smax; /* The optimum score. */ register int score; /* Current score. */ register int a; #ifdef DPAL_PRINT_COVERAGE fprintf(stderr, "_dpal_long_nopath_maxgap1_local_end called\n"); #endif CHECK_ERROR(ylen < 3, "_dpal_long_nopath_maxgap1_local_end requires ylen >= 3\n"); /* Note: S2[0] and S2[1] do not get initialized in this case. */ P0 = (int *) malloc(sizeof(int)*ylen); if (!P0) { DPAL_OOM_ERROR; } P1 = (int *) malloc(sizeof(int)*ylen); if (!P1) { DPAL_OOM_ERROR; } P2 = (int *) malloc(sizeof(int)*ylen); if (!P2) { DPAL_OOM_ERROR; } S0 = P0; S1 = P1; S2 = P2; smax = 0; /* For local alignment score can never be less than 0. */ /* Initialize the 0th row of the score matrix. */ for(j=0; j < ylen; j++) { score = in->ssm[X[0]][Y[j]]; if (score < 0) score = 0; /*S[0][j] = score;*/ S0[j] = score; } /* Set the 1st row of the score matrix. */ score = in->ssm[X[1]][Y[0]]; if(score < 0) score = 0; S1[0] = score; for(j=1; j < ylen; j++) { score = S0[j-1]; if(j>1 && (a=S0[j-2] + gap) > score)score = a; score += in->ssm[X[1]][Y[j]]; if (score < 0) score = 0; S1[j] = score; } for(i=2; i < xlen - 1; i++) { score = in->ssm[X[i]][Y[0]]; if (score < 0) score = 0; S2[0] = score; score = S1[0]; if((a=S0[0] + gap) > score) score = a; score += in->ssm[X[i]][Y[1]]; if(score < 0) score = 0; S2[1] = score; for(j=2; j < ylen; j++) { score = S0[j-1]; if((a=S1[j-2])>score) score = a; score +=gap; if((a=S1[j-1]) >score) score = a; score += in->ssm[X[i]][Y[j]]; if (score < 0 ) score = 0; S2[j]=score; } S = S0; S0 = S1; S1 = S2; S2 = S; } /* Calculate scores for last row (i = xlen-1) and find smax */ i = xlen - 1; score = in->ssm[X[i]][Y[0]]; if (score < 0) score = 0; else if (score > smax) smax = score; S2[0] = score; score = S1[0]; if((a=S0[0] + gap) > score) score = a; score += in->ssm[X[i]][Y[1]]; if(score < 0) score = 0; else if (score > smax) smax = score; S2[1] = score; for(j=2; j < ylen; j++) { score = S0[j-1]; if((a=S1[j-2])>score) score = a; score +=gap; if((a=S1[j-1]) >score) score = a; score += in->ssm[X[i]][Y[j]]; if (score < 0 ) score = 0; else if (score > smax) smax = score; S2[j]=score; } out->score = smax; out->path_length=0; free(P0); free(P1); free(P2); return; FAIL: fail_action(in, out); } /* _dpal_long_nopath_maxgap1_local */ primer3-2.3.6/src/print_boulder.h0000664000077300007730000000423412231156245015671 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of primer3 and the primer3 suite. Primer3 and the primer3 suite are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this file (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #ifndef BOULDER_OUTPUT_H #define BOULDER_OUTPUT_H 1 #include "libprimer3.h" void print_boulder(int, const p3_global_settings *, const seq_args *, const p3retval *, int exaplain_flag); void print_boulder_error(const char *err); void print_boulder_warning(const char *err); #endif primer3-2.3.6/src/oligotm_main.c0000664000077300007730000002033612231156245015473 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky. All rights reserved. This file is part of the primer3 suite and libraries. The primer3 suite and libraries are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this software (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include #include #include #include #include "oligotm.h" /* Print the melting temperature of an oligo on stdout. */ /* This program provides a command line interface to the function oligotm() in oligtm.c */ int main(int argc, char **argv) { double tm; const char *msg = "USAGE: %s OPTIONS oligo\n" "\n" "where oligo is a DNA sequence of between 2 and 36 bases\n" "\n" "and\n" "\n" "OPTIONS can include any of the the following:\n" "\n" "-mv monovalent_conc - concentration of monovalent cations in mM, by default 50mM\n" "\n" "-dv divalent_conc - concentration of divalent cations in mM, by default 0mM\n" "\n" "-n dNTP_conc - concentration of deoxynycleotide triphosphate in mM, by default 0mM\n" "\n" "-d dna_conc - concentration of DNA strands in nM, by default 50nM\n" "\n" "-tp [0|1] - Specifies the table of thermodynamic parameters and\n" " the method of melting temperature calculation:\n" " 0 Breslauer et al., 1986 and Rychlik et al., 1990\n" " (used by primer3 up to and including release 1.1.0).\n" " This is the default, but _not_ the recommended value.\n" " 1 Use nearest neighbor parameters from SantaLucia 1998\n" " *THIS IS THE RECOMMENDED VALUE*\n" "\n" "-sc [0..2] - Specifies salt correction formula for the melting \n" " temperature calculation\n" " 0 Schildkraut and Lifson 1965, used by primer3 up to \n" " and including release 1.1.0.\n" " This is the default but _not_ the recommended value.\n" " 1 SantaLucia 1998\n" " *THIS IS THE RECOMMENDED VAULE*\n" " 2 Owczarzy et al., 2004\n\n" "\n\n" "Prints oligo's melting temperature on stdout.\n"; const char *copyright = "Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006\n" "Whitehead Institute for Biomedical Research, Steve Rozen\n" "(http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky\n" "All rights reserved.\n" "\n" " This file is part of the oligotm library.\n" "\n" " The oligotm library is free software; you can redistribute it and/or modify\n" " it under the terms of the GNU General Public License as published by\n" " the Free Software Foundation; either version 2 of the License, or\n" " (at your option) any later version.\n" "\n" " The oligotm library is distributed in the hope that it will be useful,\n" " but WITHOUT ANY WARRANTY; without even the implied warranty of\n" " MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the\n" " GNU General Public License for more details.\n" "\n" " You should have received a copy of the GNU General Public License\n" " along with the oligtm library (file gpl-2.0.txt in the source\n" " distribution). If not, see http://www.gnu.org/licenses/old-licenses/gpl-2.0.txt;\n" " or write to the Free Software Foundation, Inc.,\n" " 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA\n"; char *endptr, *seq; long mv = 50, d = 50; double dv = 0, n = 0; int tm_santalucia=0, salt_corrections=0; int i, j, len; if (argc < 2 || argc > 14) { fprintf(stderr, msg, argv[0]); fprintf(stderr, "%s", copyright); return -1; } for (i=1; i < argc; ++i) { if (!strncmp("-mv", argv[i], 3)) { /* conc of monovalent cations */ if (i+1 >= argc) { /* Missing value */ fprintf(stderr, msg, argv[0]); exit(-1); } mv = strtol(argv[i+1], &endptr, 10); if ('\0' != *endptr) { fprintf(stderr, msg, argv[0]); exit(-1); } i++; } else if (!strncmp("-dv", argv[i], 3)) { /* conc of divalent cations; added by T.Koressaar */ if (i+1 >= argc) { /* Missing value */ fprintf(stderr, msg, argv[0]); exit(-1); } dv = strtod(argv[i+1], &endptr); if('\0' != *endptr) { fprintf(stderr, msg, argv[0]); exit(-1); } i++; } else if (!strncmp("-n", argv[i], 2)) { /* conc of dNTP; added by T.Koressaar */ if (i+1 >= argc) { /* Missing value */ fprintf(stderr, msg, argv[0]); exit(-1); } n = strtod(argv[i+1], &endptr); if('\0' != *endptr) { fprintf(stderr, msg, argv[0]); exit(-1); } i++; } else if (!strncmp("-d", argv[i], 2)) { if (i+1 >= argc) { /* Missing value */ fprintf(stderr, msg, argv[0]); exit(-1); } d = strtol(argv[i+1], &endptr, 10); if ('\0' != *endptr) { fprintf(stderr, msg, argv[0]); exit(-1); } i++; } else if (!strncmp("-tp", argv[i], 3)) { /* added by T.Koressaar */ if (i+1 >= argc) { /* Missing value */ fprintf(stderr, msg, argv[0]); exit(-1); } tm_santalucia = (int)strtol(argv[i+1], &endptr, 10); if ('\0' != *endptr || tm_santalucia<0 || tm_santalucia>1) { fprintf(stderr, msg, argv[0]); exit(-1); } i++; } else if (!strncmp("-sc", argv[i], 3)) { /* added by T.Koressaar */ if (i+1 >= argc) { /* Missing value */ fprintf(stderr, msg, argv[0]); exit(-1); } salt_corrections = (int)strtol(argv[i+1], &endptr, 10); if ('\0' != *endptr || salt_corrections<0 || salt_corrections>2) { fprintf(stderr, msg, argv[0]); exit(-1); } i++; } else if (!strncmp("-", argv[i], 1)) { /* Unknown option. */ fprintf(stderr, msg, argv[0]); exit(-1); } else break; /* all args processed. go on to sequences. */ } if(!argv[i]) { /* if no oligonucleotide sequence is specified */ fprintf(stderr, msg, argv[0]); exit(-1); } /* input sequence to uppercase */ seq = argv[i]; len=strlen(seq); for(j=0;j #include #include int main(int argc, const char**argv) { dpal_args a; dpal_results r; const unsigned char *s1, *s2; int tmp_ret; int i; int print_align_end = 0; /* * Print align_end_1 and align_end_2 from * dpal_results. */ int use_ambiguity_codes = 0; int use_h_matrix = 0; char mode; char *endptr; const char *msg = "\nUsage: %s [-g ] [-l ] [-m ]\n" " [-f2] [-p] [-s] [-e] " "\n\nwhere\n\n" " and are (positive) floats (.01 precision)\n" " specifying penalties for creating or lengthening a gap\n" " respectively (the penalties are subtracted from the\n" " output score).\n\n" "-a causes the scoring matrix to be modified by dpal_set_ambiguity_codes.\n\n" "-e causes the end postion of the alignment in both sequences to\n" " be printed. Do not confuse with the 'e' .\n\n" "-f1, -f2, f3\n" " force specific implementations.\n" " -f2 forces use an implementation that might provide more\n" " informative error messages, possibly at the expense\n" " of some speed.\n\n" "-h use a different scoring matrix: G and C matches = 3, A and T = 2,\n" " and mismatches = -0.5.\n" " (The default scoring matrix assigns 1 to a match,\n" " and -1 to a mismatch.)\n\n" "-p causes the alignment to be displayed on stderr.\n\n" "-s causes _only_ the score to printed.\n\n" " is the maximum allowed gap (default is 3).\n\n" " and are the sequences to be aligned.\n\n" " is one of g, G, l, or L specifying a global,\n" " global end-anchored, local, or local end-achored\n" " alignment respectively. For backward compatibility\n" " e is equivalent to G.\n\n"; if (argc < 4) { tmp_ret = fprintf(stderr, msg, argv[0]); exit(-1); } dpal_set_default_nt_args(&a); for (i=1; i < argc; ++i) { if (!strncmp("-p", argv[i], 2)) { a.debug = 1; } else if (!strncmp("-l", argv[i], 2)) { if (i+1 >= argc) { /* Missing value */ fprintf(stderr, msg, argv[0]); exit(-1); } a.gapl = (int) (strtod(argv[i+1],(char **)NULL) * -100); i++; } else if (!strncmp("-e", argv[i], 2)) { print_align_end = 1; } else if (!strncmp("-a", argv[i], 2)) { use_ambiguity_codes = 1; } else if (!strncmp("-h", argv[i], 2)) { use_h_matrix = 1; } else if (!strncmp("-g", argv[i], 2)) { if (i+1 >= argc) { /* Missing value */ fprintf(stderr, msg, argv[0]); exit(-1); } a.gap = (int) (strtod(argv[i+1],(char **)NULL) * -100); i++; } else if (!strncmp("-m", argv[i], 2)) { if (i+1 >= argc) { /* Missing value */ fprintf(stderr, msg, argv[0]); exit(-1); } a.max_gap = strtol(argv[i+1], &endptr, 10); if ('\0' != *endptr) { fprintf(stderr, msg, argv[0]); exit(-1); } i++; } else if (!strncmp("-s", argv[i], 2)) { a.score_only = 1; } else if (!strncmp("-e", argv[i], 2)) { print_align_end = 1; } else if (!strncmp("-f1", argv[i], 3)) { a.force_generic = 1; } else if (!strncmp("-f2", argv[i], 3)) { a.force_long_generic = 1; } else if (!strncmp("-f3", argv[i], 3)) { a.force_long_maxgap1 = 1; } else if (!strncmp("-", argv[i], 1)) { /* Unknown option. */ fprintf(stderr, msg, argv[0]); exit(-1); } else break; /* all args processed. go on to sequences. */ } if (use_h_matrix) dpal_set_h_nt_matrix(&a); if (use_ambiguity_codes) dpal_set_ambiguity_code_matrix(&a); if (a.score_only && a.debug) { fprintf(stderr, msg, argv[0]); exit(-1); } a.flag = -1; if ((argc - i) != 3){ /* Not enough remaining arguments. */ fprintf(stderr, msg, argv[0]); exit(-1); } s1 = (unsigned char *) argv[i]; s2 = (unsigned char *) argv[i+1]; mode = *argv[i+2]; if ('l' == mode) a.flag = DPAL_LOCAL; else if ('e' == mode || 'G' == mode) a.flag = DPAL_GLOBAL_END; else if ('g' == mode) a.flag = DPAL_GLOBAL; else if ('L' == mode) a.flag = DPAL_LOCAL_END; else { fprintf(stderr, msg, argv[0]); exit(-1); } if(print_align_end == 1) a.force_long_generic = 1; dpal(s1, s2, &a, &r); if (r.score == DPAL_ERROR_SCORE) { tmp_ret = fprintf(stderr, "Error: %s\n", r.msg); exit(-1); } if (a.score_only) { printf("%.2f\n", 0.01 * r.score); if (print_align_end) { if(r.align_end_1 >= 0) printf("align_end_1=%d ",r.align_end_1); if(r.align_end_2 >= 0) printf("align_end_2=%d\n ",r.align_end_2); } } else { printf("|%s| |%s| %c ", s1, s2, mode); printf("score=%.2f len=%d ", (0.01 * r.score), r.path_length); if (print_align_end) { if(r.align_end_1 >= 0) printf("align_end_1=%d ",r.align_end_1); if(r.align_end_2 >= 0) printf("align_end_2=%d ",r.align_end_2); } for (i=0; iALU Human ALU interspersed repetitive sequence - a consensus GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA >MIR mammalian-wide interspersed repeat (MIR) - a consensus ACAGYAYAGCATAGTGGTTAAGAGCACGGRCTCTGGAGCCAGACTGCCTGGGTTCGAATCCCGGCTCTGCCACTTACTAGCTGTGTGACCTTGGGCAAGTTACTTAACCTCTCTGTGCCTCAGTTTCCTCATCTGTAAAATGGGGATAATAATAGTACCTACCTCATAGGGTTGTTGTGAGGATTAAATGAGTTAATAYATGTAAAGCGCTTAGAACAGTGCCTGGCACATAGTAAGCGCTCAATAAATGTTRGYTATTATT >MIR2 Repetitive element - a consensus TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT >L1 Human L1 interspersed repetitive sequence - full length copy GGCGGTGGAGCCAAGATGACCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGACCATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACCACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAAAA >L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence CTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAAACAAACAACCCCATCAAAAAGTGGGCGAAGGACATGAACAGACACTTCTCAAAAGAAGACATTTATGCAGCCAAAAAACACATGAAGAAATGCTCATCATCACTGGCCATCAGAGAAATGCAAATCAAAACCACTATGAGATATCATCTCACACCAGTTAGAATGGCAATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGTGGAAGTCAGTGTGGCGATTCCTCAGGGATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTATATACCCAAAGGACTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAATGATAGACTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAATTGGAAATCATCATTCTCAGTAAACTATCGCAAGAACAAAAAACCAAACACCGCATATTCTCACTCATAGGTGGGAATTGAACAATGAGATCACATGGACACAGGAAGGGGAATATCACACTCTGGGGACTGTGGTGGGGTGGGGGGAGGGGGGAGGGATAGCATTGGGAGATATACCTAATGCTAGATGACGAGTTAGTGGGTGCAGCGCACCAGCATGGCACATGTATACATATGTAACTAACCTGCACAATGTGCACATGTACCCTAAAACTTAAAGTATAATAAAA >L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence CTAATATCCAGAATCTACAAGGAACTTAAACAAATTTACAAGAAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGYAGCCAACAAACAYATGAAAAAAAGCTCATCATCACTGGTCATTAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCAYGCCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGATGCTGGAGAGGATGTGGAGAAATAGGAATGCTTTTACACTGTTGGTGGGAGTGTAAATTAGTTCAACCATTGTGGAAGACAGTGTGGCGATTCCTCAAGGATCTAGAACCAGAAATACCATTTGACCCAGCAATCCCATTACTGGGTATATACCCAAAGGATTATAAATCATTCTRCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGCCCATCAATGATAGACTGGATAAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAAGGATGAGTTCATGTCCTTTGCAGGGACATGGATGAAGCTGGAAACCATCATTCTCAGCAAACTAACACAGGAACAGAAAACCAAACACCGCATGTTCTCACTCATAAGTGGGAGTTGAACAATGAGAACACATGGACACAGGGAGGGGAACATCACACACCGGGGCCTGTCGGGGGGTGGGGGGCTAGGGGAGGGATAGCATTAGGAGAAATACCTAATGTAGATGACGGGTTGATGGGTGCAGCAAACCACCATGGCACATGTATACCTATGTAACAAACCTGCACGTTCTGCACATGTATCCCAGAACTTAAAGTATAATAATA >L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence CTAATATCCAGAATSTACAARGAACTTAAACAAATTTACAAGAAAAAAACAAAMAACCCCATCAAAAAGTGGGCAAAGGACATGAACAGACACTTCTCAAAAGAAGACATWTATGTGGCCAATAAGCACATGAAAAAAAGCTCAACATCACTGATCATTAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCAYRCCAGTCAGAATGGCAATTATTAAAAAGTCAAGAAACAACAGATGCTGGYGAGGCTGTGGAGAAATAGGAATGCTTTTACACTGTTGGTGGGAATGTAAATTAGTTCAACCATTGTGGAAGACAGTGTGGCGATTCCTCAAGGATCTAGAACCAGAAATACCATTTGACCCAGCAATCCCATTACTGRGTATATACCCAAAGGAATATAAATCATTCTATTATAAAGACACATGCAYGTGTATGTTYATTGCAGCACTATTCACAATAGCAAAGACATGGAATCAACCYAAATGCCCATCAATGATAGACTGGATAAAGAAAATGTGGTACATATACACCATGGAATACTATGCAGCCATAAAAAAGAATGAGATCATGTCCTTTGCAGGAACATGGATGGAGCTGGAGGCCATTATCCTTAGCAAACTAAYGCAGGAACAGAAAACCAAATACCGCATGTTCTCACTTATAAGTGGGAGCTAAACGATGAGAACACATGGACACATGGAGGGGAACAACACACACTGGGGCCTGTCGGAGGGTGGAGGGTGGGAGGAGGGAGAGCATCAGGAAAAATAGCTAATGGATACTGGGCTTAATACCTGGGTGATGGGATGATCTGTGCAGCAAACCACCATGGCACACGTTTACCTATGTAACAAACCTGCACATCCTGCACATGTACCCCGGAACTTAAAATAAAAGTTGAAA >L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence CTAATATCCAGAATSTACAARGAACTTAAACAAATTTACAAGAAAAAAACAAAMAACCCCATCAAAAAGTGGGCAAAGGACATGAACAGACACTTCTCAAAAGAAGACATWTATGTGGCCAATAAGCACATGAAAAAAAGCTCAACATCACTGATCATTAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCAYRCCAGTCAGAATGGCAATTATTAAAAAGTCAAGAAACAACAGATGCTGGYGAGGCTGTGGAGAAATAGGAATGCTTTTACACTGTTGGTGGGAATGTAAATTAGTTCAACCATTGTGGAAGACAGTGTGGCGATTCCTCAAGGATCTAGAACCAGAAATACCATTTGACCCAGCAATCCCATTACTGRGTATATACCCAAAGGAATATAAATCATTCTATTATAAAGACACATGCAYGTGTATGTTYATTGCAGCACTATTCACAATAGCAAAGACATGGAATCAACCYAAATGCCCATCAATGATAGACTGGATAAAGAAAATGTGGTACATATACACCATGGAATACTATGCAGCCATAAAAAAGAATGAGATCATGTCCTTTGCAGGAACATGGATGGAGCTGGAGGCCATTATCCTTAGCAAACTAAYGCAGGAACAGAAAACCAAATACCGCATGTTCTCACTTATAAGTGGGAGCTAAAYATTGAGTACACATGGACATAAAGAAGGGAACAACAGACACTGGGGCCTACTTGAGGGTGGAGGGTGGGAAGAGGGAGAGGATCAAAAAACTACCTATCGGGTACTATGCTTATTACCTGGGTGATGAAATAATCTGTACACCAAACCCCAGCGACACGCAATTTACCTATATAACAAACCTGCACATGTACCCCCTGAATCTAAAATAAAAGTTAAAA >L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence CTAATATCCAGAATCTATAAGGAACTCAAACAAATCAGCAAGAAAAAAACAAACAATCCCATCAAAAAGTGGGCTAAGGACATGAAYAGACAMTTCTCAAAAGAAGATATRYAAATGGCCAACAAGCATATGAAAAAATGCTCAACATCACTAATTATCAGGGAAATGCAAATCAAAACCACAATGCRATACCATCTTACTCCTGCAAGAATGGCCATAATTAAAAAATCAAAAAATAATAGATGTTGGCGTGGATGTGGTGAAAAGGGAACACTTYTACACTGCTGGTGGGAATGTAAACTAGTACAACCACTATGGAAAACAGTATGGAGATTYCTTAAAGAACTAAAAGTAGAACTACCATTTGATCCAGCAATCCCACTACTGGGTATCTACCCARARGAAAAKAAGTCATTATAYGAAAAAGAYACTTGCACACRCATGTTTATAGCAGCACAATTYRCAATTGCAAAAATATGGAACCAACCCAAATGCCCATCAATCAAYRAGTGGATAAAGAAAATGTGRTATATATATACCATGGAATACTACKCAGCCATAAAAAAGAATGAAATAATGGCATTTGCAGCAACCTGGATGGARYTGGAGACYATTATTCTAAGTGAAGTAACTCAGGAATGGAAAACCAAACATYRTATGTTCTCACTTATAAGTGGGAGCTAAGCTATGAGGATGCAAAGGCGTAAGAATGATACAATGGACTTTGGGGACTCGGGAGATCGGGRGAAAGGGTGGGAGGGGGGTGAGGGATAAAAGACTACAAATTGGGTACAGTGTACACTGCTCGGGTGATGGGTGCACCAAAATCTCACAAATCACCACTAAAGAACTTATTCATGTAACCAAACACCACCTGTTCCCCAAAAACCTATTGAAATAAA >L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence CTAATATCCAGAATCTATAAGGAACTCAAACAAATCAGCAAGAAAAAAACAAACAATCCCATCAAAAAGTGGGCTAAGGACATGAAYAGACAMTTCTCAAAAGAAGATATRYAAATGGCCAACAAGCATATGAAAAAATGCTCAACATCACTAATTATCAGGGAAATGCAAATCAAAACCACAATGCRATACCATCTTACTCCTGCAAGAATGGCCATAATTAAAAAATCAAAAAATAATAGATGTTGGCGTGGATGTGGTGAAAAGGGAACACTTYTACACTGCTGGTGGGAATGTAAACTAGTACAACCACTATGGAAAACAGTATGGAGATTYCTTAAAGAACTAAAAGTAGAACTACCATTTGATCCAGCAATCCCACTACTGGGTATCTACCCARARGAAAAKAAGTCATTATAYGAAAAAGAYACTTGCACACRCATGTTTATAGCAGCACAATTYRCAATTGCAAAAATATGGAACCAACCCAAATGCCCATCAATCAAYRAGTGGATAAAGAAAATGTGRTATATATATACCATGGAATACTACKCAGCCATAAAAAAGAATGAAATAATGGCATTTGCAGCAACCTGGATGGARYTGGAGACYATTATTCTAAGTGAAGTAACTCAGGAATGGAAAACCAAACATYRTATGTTCTCACTTATAAGTGGGAGCTAAGCTATGGGTATATACGGGCATATAGAGTGGTATAATAGACAYTGGAGACTCAAAAGGTGGAGGGTAGGAGAGGGGTGAGGGATGAAAAATTACCTATTGGGTACAATGTWCACTATTCGGGTGATGGGTACACTAAAAGCCCAGACTTCACCACTAYACAATATATCCATGTAACAAAAAKGCACYTGTACCCCCTAAATYTATAAAAATAAA >L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence YTAATATCCAGAATMTATAAGGAACTCAAACAAATCAACAAGAAAAAAACAAATAATCCMATTAAAAARTGGGCAAAAGACATGAAYAGACATTTCTCAAAAGAAGACATACAAATGGCCAACARGYATATGAAAAAATGCTCAACATCACTAATCATCAGAGAAATGCAAATCAAAACYACAATGAGATATCATCTCACCCCAGTTARAATGGCTTTTATTAAAAAGACAAAAAATAACARATGCTGGYGAGGATGTGGAGAAAAGGGAACTCTTAYAYACTGTTGGTGGGAATGTAAATTAGTACARCCAYTATGGAAAACAGTTTGGAGRTTYCTCAAAAAACTAAAAATAGARCTACCATATRATCCAGCAATCCCACTRCTGGGTATATAYCCAAAAGAAAGGAAATCAGTATATYAAARAGATACCTGCACTCCCATGTTTATTGCAGCACTATTCACAATAGCHAAGATTTGGAATCAACCTAAGTGTCCATCAACRGATGAATGGATAAAGAAAATGTGGTACATATACACAATGGAGTACTATTCAGCCATAAAAAAGAATGARATCYTGTCATTTGCAGCAACATGGATGGAACTGGAGGTCATTATGTTAAGTGAAATAAGYCARGMACARAAAGACAAACAYTGCATGTTCTCACTTATTTGTGGGATCTAAAAATCAAAACAATTGAACTCATGGAGATAGAGAGTAGAAGGATGGTTACCAGAGGCTGGGAAGGGTAGTNGGAGGATTGGGGGGNGGKGRRGAGGGATGGTTAATGGGTACAAAAAAATAGTTAGAAAGAATGAATAAGACCTAGTATTTGATAGCACAACAAGGTGACTATAGTCAATAATAATTTAATTGTACATTTTAAAATAACTAAAAGAGTATAATTGGATTGTTTGTAACACAAAGGATAAATGCTTGAGGGGATGGATACCCCATTTTCCATGATGTGATTATTACACATTGCGTGCCTGTATCAAAACATCTCATGTACCCCATAAATATATACACCTACTATGTACCCACAAAAATTAAAA >L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence TTAATATCCAAAATATATAAGGAACTCAAACAACTCAACAAGAARAAAACAAATAACCCAATTAAAAAATGGGCAAARGACCTGAATAGACATTTYTCAAAAGAAGACATACAAATGGCCAACAGATATATGAAAAAATGCTCAACATCACTAATCATCAAGGAAATGCAAATTAAAACCACAATGAGATATCACCTCACACCTGTTAGAATGGCTATTATCAAAAAGACAGAAAATAATAAATGTTGGYGAGGATGTGGAGAAAAGGGAACTATTGTACACTGTTGGTGGGAATGTAAATTAGTAYAGCCAYTATGGAAAACAGTATGGAGGTTCCTCAAAAAAYTAAAAATARAACTACCATATGATYCAGCAATCCCACTWCTGGGTATATATCCAAARGAATTGAAATCAGTATGTYGAAGAGATAYCTGCACTCCCATGTTTAYTGCAGCAYTATTCACAATAGCCAAGATATGGAAWCAACCTAAGTGTCCATCAAYGGAWGAATGGATAAAGAAAATGTGGTATATATACACAATGGAATACTATTCAGCCATAAAAAAGAATGAAATCCTGTCATTTGYARCAACATGGATGAACCTGGAGGACATTATGCTAAGTGAAATAAGCCAGGCACAGAAAGACAAATACCGCATGTTCTCACTCATATGTGGAAGCTAAAAAAGTTGATCTCATAGAAGTAGAGAGTAGAATAGTGGTTACTAGAGGCTGGGAAGGGTAGGGRGAGGGGGGRATAGGGAGAGGTTGGTTAAWGGRTACAAAATTACAGTTAGATAGGAGGAATAAGTTCTAGTGTTCTGTAGCACCGTAGGGTGACTATAGTTAACAAYAATTTATTGTATATTTTCAAATAGCTAGAAGAGAGGATTTTGAATGTTCCCAACACAAAGAAATGATAAATGTTTGAGGTGATGGATATGCTAATTACCCTGATTTGATCATTACACAWTGTATACATGTATCGAAATATCACACTGTACCCCATAAATATGTACAATTATTATGTGTCAATTAAAAA >L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence TTAATATCCAAAATATATAAGGAACTCAAACAACTCAACAAGAARAAAACAAATAACCCAATTAAAAAATGGGCAAARGACCTGAATAGACATTTYTCAAAAGAAGACATACAAATGGCCAACAGATATATGAAAAAATGCTCAACATCACTAATCATCAAGGAAATGCAAATTAAAACCACAATGAGATATCACCTCACACCTGTTAGAATGGCTATTATCAAAAAGACAGAAAATAATAAATGTTGGYGAGGATGTGGAGAAAAGGGAACTATTGTACACTGTTGGTGGGAATGTAAATTAGTAYAGCCAYTATGGAAAACAGTATGGAGGTTCCTCAAAAAAYTAAAAATARAACTACCATATGATYCAGCAATCCCACTWCTGGGTATATATCCAAARGAATTGAAATCAGTATGTYGAAGAGATAYCTGCACTCCCATGTTTAYTGCAGCAYTATTCACAATAGCCAAGATATGGAAWCAACCTAAGTGTCCATCAAYGGAWGAATGGATAAAGAAAATGTGGTATATATACACAATGGAATACTATTCAGCCATAAAAAAGAATGAAATCCTGTCATTTGYARCAACATGGATGAACCTGGAGGACATTATGCTAAGTGAAATAAGCCAGGCACAGAAAGACAAATACTGCATGATCTCACTTATATGTGGAATCTAAAAAAGTCAAAYTCATAGAAACAGAGAGTAGAATGGTGGTTACCAGGGGCTGGGGRAKGGGGGAAATGGGAAGATGTTGGTCAAAGGGTACAAAGTTKCAGTTATGTARGATGAATAAGTTCTRGAGAYCTAATGTACAGCATGGTGACTATAGTTAATAATACTGTATTGTATACTTGAAATTTGCTAAGAGAGTAGATYTTAAGTGTTCTCACCACANACAAATGGTAACTATGTGAGGTGATGGATATGTTAATTAGCTTGAYTGTGGTAATCATTTCACAATGTATACATATATCAAAACATCACGTTGTACACCTTAAATATATACAATTTTTATTTGTCAATTATACCTCAATAAAGCTGRAAA >L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence TTAATATCCAAAATATATAAAGAACTCYTACAACTCAACAAAAAAAAAACAAACAACCCAATTAAAAAATGGGCAAARGACTTGAATAGACATTTCTCCAAAGAAGAYATAYAAATGGCCAATAAGYACATGAAAARATGCTCAACATCAYTAATCATTAGGGAAATGCAAATCAAAACCACAATGAGATAYCACCTYACACCCATTAGGATGGCTATTATYAAAAAAMCAGAAAATAACAAGTGTTGRYGAGGATGTGGAGAAATTGGAACCCTTRTRCATTGCTGGTGGGAATGTAAAATGGTRCARCCACTRTGGAAAACAGTATGGHRGTTCCTCAAAAAATTAAAAATAGAATTACCATATGACCCAGCAATYCCACTYCTRGGTATATACCCAAAAGAATTGAAATCATGTTCYYAHAAAGATAYTTGTACACCMATGTTCATAGCAGCATTATTCAYAATAGCCAAAAGGTGGAAACAACCCAAATGTCCATCAATGRWTGAATGGATAAACAAAATGTGGTATATACATACAATGGAATATTATTCAGCCTTAAAAAGGAAGGAAATYCTGACAYATGCTACAACATGGATGAACCTTGARGACATTATGCTAAGTGAAATAAGCCAGTCACAAAAGGACAAATACTGCATGATTCCACTTATATGAGGTATCTAAAATAGTCAAACTCATAGAARCAGAGAGTAGAATGGTGGTTGCCAGGGGCTGGGGGRAGGGGGAAATGGGGAGTTGCTGTTCAATGGGTATAAAGTTTCAGTTATGCAAGATGAATAAGTTCTAGAGATCTGCTGTACAACATTGTGCCTATAGTTAATAATACTGTATTGTACACTTAAAAWTTGTTAAAAGRGTAGATCTCATGTTAAGTGTTCTTATCGCACAACAAAAAAATGGGGAACTTTTGGRRGTGATGGATATGTTCATTATCTTGATTGTGGTGATGGTWTCACGGGTGTNTACATATGTCAAAACTCATCAAATTGTACACWTTAAATATATGCAGTTTTTAGTGTATAATTATACCTCAACAAAGCTGTTTT >L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence TTAATATCCAAAATATATAAAGAACTCYTACAACTCAACAAAAAAAAAACAAACAACCCAATTAAAAAATGGGCAAARGACTTGAATAGACATTTCTCCAAAGAAGAYATAYAAATGGCCAATAAGYACATGAAAARATGCTCAACATCAYTAATCATTAGGGAAATGCAAATCAAAACCACAATGAGATAYCACCTYACACCCATTAGGATGGCTATTATYAAAAAAMCAGAAAATAACAAGTGTTGRYGAGGATGTGGAGAAATTGGAACCCTTRTRCATTGCTGGTGGGAATGTAAAATGGTRCARCCACTRTGGAAAACAGTATGGHRGTTCCTCAAAAAATTAAAAATAGAATTACCATATGACCCAGCAATYCCACTYCTRGGTATATACCCAAAAGAATTGAAATCATGTTCYYAHAAAGATAYTTGTACACCMATGTTCATAGCAGCATTATTCAYAATAGCCAAAAGGTGGAAACAACCCAAATGTCCATCAATGRWTGAATGGATAAACAAAATGTGGTATATACATACAATGGAATATTATTCAGCCTTAAAAAGGAAGGAAATYCTGACAYATGCTACAACATGGATGAACCTTGARGACATTATGCTAAGTGAAATAAGCCAGTCACAAAAGGACAAATACTGTATGATTCCACTTATATGAGGTACCTAGAGTAGTCAAATTCATAGAGACAGAAAGTAGAATGGTGGTTGCCAGGGGCTGGGGGGAGGGGGGAATGGGGAGTTAKTGTTTAATGGGTACAGAGTTTCAGTTTGGGAAGATGAAAAAGTTCTGGAGATGGATGGTGGTGATGGTTGCACAACAATGTGAATGTACTTAACGCCACTGAACTGTACACTTAAAAATGGTTAAAATGGTAAATTTTATGTTATGTATATTTTACCACAATTAAAA >L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence YTTGTATCCAGAATATATAAAGAACTCTTACAACTCAACAATAAAAAAACAAACAACCTAATTAAAAAATGGGCAAAAGAYTTGAATAGATATTTCTCCAAAGAAGATATAYAAATGGCCAATAAGCACATGAAAAGATRCTCAACATCATTAGTCATCAGGGAAATGCAAATYAAAACCACAATGAGATAYCACTTCACACCCAYTAGAATGGCTAAAATTAAAAAGACAGRMAATAMCAARTGTTGGYRAGGATGTRGAGAAAYTGGAACHCTCATACAYTGCTGGTGGGAATGTAAAATGGTACARCYACTTTGGAAAACAGTTTGGCAGTTCCTCAAAAAGTTAAMAATAGAGTTACCATATGACCCAGCAATTYCACTCCTAGGTATWTACCCAAGAGAAATGAAAACATAYRTCCAYACAAAAACTTGTACACAAATGTTCATAGCAGCATTATTCATAATAGCCAAAAAGTGGAAACAACCCAAATGTCCATCAATRATWGAATGGATAAACAAAATGTGGTATATCCATACAATGGAATATTATTCAGCMATAAAAAGGAATGAAGTAMTGATMYATGCAACAACATGGATGAACCTTGAAAACATTATGCTAAGTGAAAGAAGCCARRCACAAAAGRCCACATATTGTATGATTCCATTTATATGAAATGTCCAGAATAGGCAAATCCATAGAGACAGAAAGTAGATTAGTGGTTGCCAGGGGCTGGGGGRAAGGGGAAATGGGGAGTGACTGCTAATGGGTACGGGGTTTCTTTTTGGGGTGATGAAAATGTTCTAAAATTAGATAGTGGTGATGGTTGCACAACTYTGTGAATATACTAAAAACCACTGAATTGTACACTTTAAAAGGGTGAATTTTATGGTATGTGAATTATATCTCAATAAARCTAT >L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence YTTGTATCCAGAATATATAAAGAACTCTTACAACTCAACAATAAAAAAACAAACAACCTAATTAAAAAATGGGCAAAAGAYTTGAATAGATATTTCTCCAAAGAAGATATAYAAATGGCCAATAAGCACATGAAAAGATRCTCAACATCATTAGTCATCAGGGAAATGCAAATYAAAACCACAATGAGATAYCACTTCACACCCAYTAGAATGGCTAAAATTAAAAAGACAGRMAATAMCAARTGTTGGYRAGGATGTRGAGAAAYTGGAACHCTCATACAYTGCTGGTGGGAATGTAAAATGGTACARCYACTTTGGAAAACAGTTTGGCAGTTCCTCAAAAAGTTAAMAATAGAGTTACCATATGACCCAGCAATTYCACTCCTAGGTATWTACCCAAGAGAAATGAAAACATAYRTCCAYACAAAAACTTGTACACAAATGTTCATAGCAGCATTATTCATAATAGCCAAAAAGTGGAAACAACCCAAATGTCCATCAATRATWGAATGGATAAACAAAATGTGGTATATCCATACAATGGAATATTATTCAGCMATAAAAAGGAATGAAGTAMTGATMYATGCAACAACATGGATGAACCTTGAAAACATTATGCTAAGTGAAAGAAGCCARRCACAAAAGRCCACATATTGTATGATTCCAACTATATGACATTCTGAAAAAGGNAAAACTATGGAGACAAGTAAAAAGATCAGWGATTNCTAGAGKGASTRGGARRGGGAGGGAAGAATAGGTGGAGCNCAGGGGATTTTTAGGGCAGCGAAACTATTCTGTATGATACTATAATGGTGGATACATGACATTATACATTTGTCAAAAYCCATAGAACTGTACAAYACAAAGAGTGAACCCTAATGTATGGACTTTAGTTAATAATAATGTATCAATGTTGGTTCATCAATTGTAACAAATGTACCACATTAATGCAAGATGTTAATAATAGGGTAAACTRTTGTGTGGGGGAGGGAGTATATGGGAACTCTCTGTACTTTCTGCTCAATTTTTCTGTAAACCTAAAACTGYTTCAAAAAATAAAGTCTATTAATAA >L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence YTTGTATCCAGAATATATAAAGAACTCTTACAACTCAACAATAAAAAAACAAACAACCTAATTAAAAAATGGGCAAAAGAYTTGAATAGATATTTCTCCAAAGAAGATATAYAAATGGCCAATAAGCACATGAAAAGATRCTCAACATCATTAGTCATCAGGGAAATGCAAATYAAAACCACAATGAGATAYCACTTCACACCCAYTAGAATGGCTAAAATTAAAAAGACAGRMAATAMCAARTGTTGGYRAGGATGTRGAGAAAYTGGAACHCTCATACAYTGCTGGTGGGAATGTAAAATGGTACARCYACTTTGGAAAACAGTTTGGCAGTTCCTCAAAAAGTTAAMAATAGAGTTACCATATGACCCAGCAATTYCACTCCTAGGTATWTACCCAAGAGAAATGAAAACATAYRTCCAYACAAAAACTTGTACACAAATGTTCATAGCAGCATTATTCATAATAGCCAAAAAGTGGAAACAACCCAAATGTCCATCAATRATWGAATGGATAAACAAAATGTGGTATATCCATACAATGGAATATTATTCAGCMATAAAAAGGAATGAAGTAMTGATMYATGCAACAACATGGATGAACCTTGAAAACATTATGCTAAGTGAAAGAAGCCARRCACAAAAGRCCACATATTGTATGATTCCATTTACATAACATTCTTAAAATGACAAAATTWTAGARWTGRAGARCAGATTCCTGGTTGCCAGGGGTTAGGGACGGGGGTGGGGGTGGAGGGAGGTGGGCATSSTGGAGTTCCCTGTGGTGATGGAAATGTTCTGTATCTTCACTGTCACTGTATCAATGTCAATATCCTGGTTGTGATAYTGTAYTATAGTTTTGTAAGATGTTACCATTGGGGGAAGCTGGGTGAAGGGTACACGGGATCTCTCTGTAYTATTTCTTACAATTGTCTGTGAGTKTAYAATTATTTCAAAATAAAGTTTAATTTAAA >L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence YTTGTATCCAGAATATATAAAGAACTCTTACAACTCAACAATAAAAAAACAAACAACCTAATTAAAAAATGGGCAAAAGAYTTGAATAGATATTTCTCCAAAGAAGATATAYAAATGGCCAATAAGCACATGAAAAGATRCTCAACATCATTAGTCATCAGGGAAATGCAAATYAAAACCACAATGAGATAYCACTTCACACCCAYTAGAATGGCTAAAATTAAAAAGACAGRMAATAMCAARTGTTGGYRAGGATGTRGAGAAAYTGGAACHCTCATACAYTGCTGGTGGGAATGTAAAATGGTACARCYACTTTGGAAAACAGTTTGGCAGTTCCTCAAAAAGTTAAMAATAGAGTTACCATATGACCCAGCAATTYCACTCCTAGGTATWTACCCAAGAGAAATGAAAACATAYRTCCAYACAAAAACTTGTACACAAATGTTCATAGCAGCATTATTCATAATAGCCAAAAAGTGGAAACAACCCAAATGTCCATCAATRATWGAATGGATAAACAAAATGTGGTATATCCATACAATGGAATATTATTCAGCMATAAAAAGGAATGAAGTAMTGATMYATGCAACAACATGGATGAACCTTGAAAACATTATGCTAAGTGAAAGAAGCCARRCACAAAAGRCCACATATTGTATGATTCCATTTATATAACATTCTTAAAACGACAAAACTATAGAGATGGAGAACAGATTAGTGRTTGCCAGGKGTTAGGGATRCAGGRAGGAGGTGGATGTGRYTATAAARRGGTAGCATGAGRGAATYTTTATGGTGATGGAACWGTTCTGTATCTTGAYTGTGGTGRTGGTTACACGAATNTATACATGTGATAAAATTGCATAGAACTAAATACACACACACACATAAGTACAAGTAAAACTGGCGAAATCTGAATAAGATTRATGGATTGTAYCAATGTCAATTTCCTGGTTKTGATATTGTAYTATAGTTTTCCAAGATGTTACCATTGGGGGAAGCTGGGTGAAGGGTACACGGGATCTCTCTGTAYTATTTCTTACAATTGTCTGTGAGTKTAYAATTATTTCAAAATAAAGTTTAATTTAAA >L1ME2 3'-end of L1 repeat (subfamily L1ME2) - a consensus sequence YTTGTATCCAGAATATATAAAGAACTCTTACAACTCAACAATAAAAAAACAAACAACCTAATTAAAAAATGGGCAAAAGAYTTGAATAGATATTTCTCCAAAGAAGATATAYAAATGGCCAATAAGCACATGAAAAGATRCTCAACATCATTAGTCATCAGGGAAATGCAAATYAAAACCACAATGAGATAYCACTTCACACCCAYTAGAATGGCTAAAATTAAAAAGACAGRMAATAMCAARTGTTGGYRAGGATGTRGAGAAAYTGGAACHCTCATACAYTGCTGGTGGGAATGTAAAATGGTACARCYACTTTGGAAAACAGTTTGGCAGTTCCTCAAAAAGTTAAMAATAGAGTTACCATATGACCCAGCAATTYCACTCCTAGGTATWTACCCAAGAGAAATGAAAACATAYRTCCAYACAAAAACTTGTACACAAATGTTCATAGCAGCATTATTCATAATAGCCAAAAAGTGGAAACAACCCAAATGTCCATCAATRATWGAATGGATAAACAAAATGTGGTATATCCATACAATGGAATATTATTCAGCMATAAAAAGGAATGAAGTAMTGATMYATGCAACAACATGGATGAACCTTGAAAACATTATGCTAAGTGAAAGAAGCCARRCACAAAAGRCCACATATTGTATGATTCCATTTATATGAAATTCWAGAACAGGCAAAACTAATCTATAATGNTAGAAATCAGAATAGTGGTTGCCTCTGGKGAGGGTRAATGACTGGRAAGGGRCATGAGGGAATTTTCTGGGGTGATGGAAATGTTCTATATCTTGATCGGGGTGGTGGTTACACGAGTGTATACATTTGTCAAAACTCATCGAACTGTACACTTAAAATCTGTGCATTTTACTGTATGTAAATTATAYCTCAATTTTAAAAAA >L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence YTTGTATCCAGAATATATAAAGAACTCTTACAACTCAACAATAAAAAAACAAACAACCTAATTAAAAAATGGGCAAAAGAYTTGAATAGATATTTCTCCAAAGAAGATATAYAAATGGCCAATAAGCACATGAAAAGATRCTCAACATCATTAGTCATCAGGGAAATGCAAATYAAAACCACAATGAGATAYCACTTCACACCCAYTAGAATGGCTAAAATTAAAAAGACAGRMAATAMCAARTGTTGGYRAGGATGTRGAGAAAYTGGAACHCTCATACAYTGCTGGTGGGAATGTAAAATGGTACARCYACTTTGGAAAACAGTTTGGCAGTTCCTCAAAAAGTTAAMAATAGAGTTACCATATGACCCAGCAATTYCACTCCTAGGTATWTACCCAAGAGAAATGAAAACATAYRTCCAYACAAAAACTTGTACACAAATGTTCATAGCAGCATTATTCATAATAGCCAAAAAGTGGAAACAACCCAAATGTCCATCAATRATWGAATGGATAAACAAAATGTGGTATATCCATACAATGGAATATTATTCAGCMATAAAAAGGAATGAAGTAMTGATMYATGCAACAACATGGATGAACCTTGAAAACATTATGCTAAGTGAAAGAAGCCARRCACAAAAGRCCACATATTGTATGATTCTATTTGCATAAAATACAAAACCAGGCAAAACTAATCAATGNTGTTAGAAGTCAGGATAGTGGTTGTTCTGGGAATAGTAATGACTGTAAGGRARCATGAGAGGGCTTTCTGGGGTGCTGGTAATGTTCTGTTTCTTGATCTGGGTGCTGGTTACACGGCTGTGTTCARTTTGTGAAAATTCATYAAGCTGTACACTTATGATWTATGCAYTTTTATGTATGTATATTATAYYTCAATAAAAA >THE1b consensus sequence TGATATGGTTTGGCTGTGTCCCCACCCAAATCTCATCTTGAATTGTAGCTCCCATAATTCCCACGTGTCGTGGGAGGGACCCGGTGGGAGGTAATTGAATCATGGGGGCGGGTCTTTCCCGTGCTGTTCTCGTGATAGTGAATAAGTCTCACGAGATCTGATGGTTTTATAAAGGGGAGTTYCCCTGCACANGCTCTCTTGCCTGCCGCCATGTAAGACGTGMCTTTGCTCCTCCTTCGCCTTCYGCCATGATTGTGAGGCCTCCCCAGCCATGTGGAACTGTGAGTCCATTAAACCTCTTTYCTTTATAAATTACCCAGTCTCGGGTATGTCTTTATTAGCAGCATGAGAACGGACTAATACA >MSTa consensus sequence TGATATGGTTTGGATCTGTGTCCCCACCCAAATCTCATGTTGAATTGTAATCCCCAATGTTGGAGGTGGGGCCTGGTGGGAGGTGATTGGATCATGGGGGCGGATTYCTCATGAATGGTTTAGCACCATCCCCTTGGTGCTGTYCTCGTGATAGTGAGTGAGTTCTCATGAGATCTGGTCGTTTAAAAGTGTGTGGCACCTCCCYCCTCTCTCTCTTGCTCCTGCTCTCGCCATGTGACGTGCCTGCTCCCCCTTCGCCTTCCGCCATGATTGNAAGCTTCCTGAGGCYTCCCCAGAAGCCGAGCAGATGCCAGCGCCATGCTTCCTGTACAGCCTGCAGAACCGTGAGCCAATTAAACCTCTTTTCTTTATAAATTACCCAGTCTCAGGTATTTCTTTATAGCAATGTGAGAACAGACTAATACA >MSTc consensus sequence TGCTACGGTTTGAATGTTTGTCCCCTCCAAAACTCATGTTGAAACTTAATCCCCAATGTGGCAGTATTGAGAGRTGGGGCCTTTRAGAGGTGATTGGGTCATGAGGGCTCTGCCCTCATGAATGGATTAATGGATTAACGTATTAATGGATTAATTGGTTATCACGGGAGTGGGATCGGTGGCTCTATCATAAAARYCATTTTGNCTCTCGNRTGNGCCCCTTCTTGCCCTTTCACGCCTTCCGCCATGTTATGACAGAGCACAAGGCCCTCACCAGAAACCAGATGCAGCCGCCATGATCTTGGACTTCCCAGCCTSCAGAACCGTGAGCAAAATAAACCTCTTTTCTTTATAAATTACCCAGTCTATGGTATTCCGTTAAAGCAGCACGAACGGACTAAGACA >MLT1a (MLT1a subfamily) - consensus sequence TGCTATGGACTGAATGTTTGTGTCCCCCCAAAATTCATATGTTGAAGCCCTAATCCCCAATGTGATGGTATTAGGAGGTGGGGCCTTTGGGAGGTGATTAGGATTAGATGAGGTCATGAGGGCGGGGCCCTCATAATGGGATTAGTGCCCTTATAAAAGAGACCYCAGAGAGCTCCCTTGCCCCTTCCGCCATGTGAGGACACAGTGAGAAGGCGCCGTCTACGAACCAGGGAATGAGCCCTCACCAGAAACTGAATCTGCCGGCGCCTTGATCTTGGACTTCCCAGCCTCCAGAACTGTGAGAAATAAATTTCTGTTGTTTAAGCTACCCAGTCTATGGTATTTTGTTATAGCAGCCCGAACAGACTAAGACA >MLT1b (MLT1b subfamily) - consensus sequence TGTTATGGGCTGAATTGTGTCCCCCCAAAATTCATATGTTGAAGTCCTAACCCCCAGTACCTCAGAATGTGACTGTATTTGGAGATAGGGTCTTTAAAGAGGTAATTAAGTTAAAATGAGGTCATTAGGGTGGGCCCTAATCCAATATGACTGGTGTCCTTATAAGAAGAGGAAATTWGGACACAGACACGCACAGAGGRAAGRCCATGTGARGACACAGGGAGAAGGCGGCCATCTRCAAGCCAAGGAGAGAGGCCTCAGAAGAAACCAACCCTGCCGRCACCTTGATCTCGGACTTCYAGCCTCCAGAACTGTGAGAAAATAAATTTCTGTTGTTTAAGCCACCCAGTCTGTGGTACTTTGTTACGGCAGCCCTAGSAAACTAATACA >MLT1c (MLT1c subfamily) - consensus sequence TGTTATGGGTTGAATTGTGTCCCCCCAAAATTGATATGTTGAAGTCCTAACCCCTAGTACCTCAGAATGTGACCTTATTTGGAAATAGGGTCWTTGCAGATGTAATTAGTTAAGATGAGGTCATACTGGAGTAGGGTGGGCCCTAAATCCAATATGACTGGTGTCCTTATAARAAGAGGAAATTTGGACACAGACACGCACACGGGGAGAAGGCCATGTGAAGACGGAGGCAGAGATTGGAGTGATGCAKCTACAAGCCAAGGAACGCCAARGRYTGCCAGCAAACCACCAGAAGCTAGGAAGAGGCAAGGAACAGATTCTCCCTCACAGCCYTCAGAGGARRCCAGCCCTGCCGACACCTTSATCTCGGACTTCTGGCCTCCAGAACTGTGAGAGAATAMATTTCTGTTGTTTAAGCCACSCAGTTTGTGGTACTTTGTTACGGCAGCCCYAGGAAACTAATACA >MLT1d (MLT1d subfamily) - consensus sequence TGTGGTAGGCWGAATAATGGCTCCCCAAAGATGTCCACGTCCTAATCCCCAGAACCTGTGAATATGTTACCTTACATGGCAAAAGGGACTTTGCAGATGTGATTAAGTTAAGGATCTTGAGATGGGGAGATTATCCTGGATTATCCGGGTGGGCCCAATGTAATCACAAGGGTCCTTAWAAGAGGGAGGCAGAGGGTCAGAGTCAGAAGAAGGAGATGTGACGATGGAAGCAGRRAGNGAAAACTCAACGTTGCTGGCTTTGAAGATGGAGGAAGGGGCCATGAGCCAAGGAATGCGGGCAGCCTCTAGAAGCTGGAAAAGGCAAGGAAACGGATTCTCCCCTAGAGCCTCCAGAARGAACGCGGCCCTGCCGACACCTTGATTTTAGCCCAGTGAGACYCATTTTGGACTTCTGACCTCCAGAACTGTAAGATAATAAATTTGTGTTGTTTTAAGCCACTAAGTTTGTGGTAATTTGTTACAGCAGCMAYAGGAAACTAATACA >MLT1e (MLT1e subfamily) - consensus sequence TGTGGTAGGCAGAATTCTAARATGMTCCCAATGATCCTCGCCTCCTGGCGTAATCTCCTTGAGTGTGAGTAGGACCTGTGACTTGCTTCTAGCCAACGGAATATGGCAAAGGTGATGARATRTCACGTGATTACGCGTACGTGATTATGTAASATTCAGTCTTTGMCGTCATTCTTGCCGAGAGACTCTCCTSCTGGTYTTGAAGAAGTAAGCTGCCACGTCATGAGNNNNCNNANNAGARYGCCGCAAGGCAAGGGNNTCTAGAGCTGAGAGTCGCCCTTACTGATGGGCAGCAAGAAGCAAGCCACCTCAGTCCTACAGCCGCAAAGAACTGAATTCTGCCAACAACCTAGTGAGCTTGGAAGCAGATCCTGCCCAGTCGAGCCTCCAGATGAGANCGCAGCCCTGGCTGACGCCTTGACTGCAGCCTTGNTAGACCTTGAGCAGAGGACCCAGCTAAGCCGTGCCCAGACTCCTGACCCACAGAAACTGTGAGATAATAAATGTGTGTTGTTTTAAGCCGCTAAGTTTGTGGTAATTTGTTACGCAGCAATAGAAAACTAACACA >MLT1f (MLT1f subfamily) - consensus sequence TGTGGTAGCCAGCCTCCAAGATGGCCCCCAATGATCCCTGCTCCTGGTATTCACAYCCTTGTATGGTCYCYTCCTACATTGARYYAGGGCTRGTCTGTGTGACCAATAGAATAGGGCAGAAGTGATGGCGTGTSACTTCCAAGAYTARGTCAYAAAWAACACTGTGGYTTCTGCYTTGNTCTCTTCGGGCTACTCACTCTGGGGGAAGCCAGCTGCCATGCTATGAAGACACTCAAGCAGCCTATGGAGAAGTCCACGTGGSAAGGAACTGAGGTCTCCTGCCAACAGCCAGCTTCGACYTGCCAGCCATGTGAGTGAGCCATCTTGGAAGCGGATCCTCCAGCCCCAGTYAAGCCTTCAGATGACTGCAGCCCCGGCTGACATCTTGACTGCAACCTCATGAGAGACCCTGAGCCAGAACTACCCAGCTAAGCTGCTCCTARATTCCTGACCCACAGAAACTGTGAGATAATAAATGTTTRTTGTTTTAAGCCACTAAGTTTTGGGGTAATTTGTTACGCAGCAATAGATAACTAATACA >THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus GTAAATTGGTACCAGTAGAGTGGGGCGCTGCTGAAAAGATACCCGAAAATGTGGAAGCGACTTTGGAACTGGGTAACAGGCAGAGGTTGGAACAGTTTGGAGGGCTCAGAAGAAGACAGGAAAATGTGGGAAAGTTTGGAACTTCCTAGAGACTTGTTGAATGGCTTTGACCAAAATGCTGATAGTGATATGGACAATAAAGTCCAGGCTGAGGTGGTCTCAGATGGAGATGAGGAACTTGTTGGGAACTGGAGCAAAGGTGACTCTTGTTATGTTTTAGCAAAGAGACTGGCGGCATTTTGCCCCTGCCCTAGAGATTTGTGGAACTTTGAACTTGAGAGAGATGATTTAGGGTATCTGGCGGAAGAAATTTCTAAGCAGCAAAGCGTTCAAGAGGTGACTTGGGTGCTGTTAAAGGCATTCAGTTTTAAAAGGGAAGCAGAGCATAAAAGTTCGGAAAATTTGCAGCCTGACAATGCGATAGAAAAGAAAATCCCATTTTCTGAGGAGAAATTCAAGCCGGCTGCAGAAATTTGCATAAGTAACGAGGAGCCGAATGTTAATCCCCAAGACAATGGGGAAAATGTCTCCAGGGCATGTCAGAGATCTTCGCGGCAGCCCCTCCCATCACAGGCCCGGAGGCCTAGGAGGAAAAAATGGTTTCGTGGGCCAGGCCCAGGGTCCCCGTGCTGTGTGCAGCCTAGGGACTTGGTGCCCTGCGTCCCAGCCGCTCCAGCCATGGCTAAAAGGGGCCAACGTACAGCTCGGGCCGTGGCTTCAGAGGGTGCAAGCCCCAAGCCTTGGCAGCTTCCACGTGGTGTTGAGCCTGCGGGTGCACAGAAGTCAAGAATTGAGGTTTGGGAACCTCCGCCTAGATTTCAGAGGATGTATGGAAACGCCTGGATGTCCAGGCAGAAGTTTGCTGCAGGGGCGGGGCCCTCATGGAGAACCTCTGCTAGGGCAGTGCGGAAGGGAAATGTGGGGTCGGAGCCCCCACACAGAGTCCCTACTGGGGCACTGCCTAGTGGAGCTGTGAGAAGAGGGCCACCGTCCTCCAGACCCCAGAATGGTAGATCCACCGACAGCTTGCACCGTGCGCCTGGAAAAGCCGCAGACACTCAACGCCAGCCCGTGAAAGCAGCCAGGAGGGAGGCTGTACCCTGCAAAGCCACAGGGGCGGAGCTGCCCAAGACCATGGGAACCCACCTCTTGCATCAGCGTGACCTGGATGTGAGACATGGAGTCAAAGGAGATCATTTTGGAGCTTTAAGATTTGACTGCCCCGCTGGATTTCGGACTTGCATGGGGCCTGTAGCCCCTTTGTTTTGGCCAATTTCTCCCATTTGGAATGGCTGTATTTACCCAATGCCTGTACCCCCATTGTATCTAGGAAGTAACTAACTTGCTTTTGATTTTACAGGCTCATAGGCGGAAGGGACTTGCCTTGTCTCAGATGAGACTTTGGACTGTGGACTTTTGAGTTAATGCTGAAATGAGTTAAGACTTTGGGGGACTGTTGGGAAGGCATGATTGGTTTTGAAATGTGAGGACATGAGATTTGGGAGGGGCCAGGGGCGGAA >MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus GAAWATTGGTACTGAGGAGTGGAGCATTGCTATAAAGATACCTGAAAATGTGGAAGCGACTTTGGAACTGGGTAACAGGCAGAGGTTGGAAGAGTTTGGAGGGCTCAGAAGAAGACAGGAAGATGAGGGAAAGTTTGGAACTTCTTAGAGACTTGTTAAATGGTTGTGACCAAAATGCTGATAGTGATATGGACAGTAAGGGCCAGGCTGACGAGGTCTCAGATGGAAATGAGGAACTTATTGGGAACTGGAGCAAAGGTCACTCTTGTTATACATTAGCAAAGAGCTTGGCTGCATTTTGCCCCTGCCCTAGAGATTTGTGGAAGTTTGAACTTGAGAGTGATGATCTAGGGTATCTGGCGGAAGAAATTTCTAAGCAGCAAAGCGTTCAAGATGTGACCTGGCTGCTTTTAACAGCTTACAGTCATATGCGAGAGCAAAGAAATCACTTAAAGTTGGAATTTATATTTAAAAGGGAAGCAGAGCGTAAAAGTTTGGAAAATTTGCAGCCTGGCCATGTGATAGAAAAGAAAAACCCGTTTTCTGGAGAGAAATTCAAGCAGGCTGCGGAGCGACCGTTTGCTAAAGAGATTAGCATAACTAAAAGGAAGCCAAGTGCTGATAGCCAAGACAATGGGAAAAAGGCCTCGAAGGCATTTCAGAAATCTTCGAGGTGGTCCTTCCCATCACAGGCCCAGAGGCCTAGGAGGACTGAATGGTTTCGTGGGCCAGGCCCAGGGCCCCGCTGCCCTGTGCAGCCTCGGGACACTGCTCCCTGCATCCCGGCTGCTYCGGCTCCAGCCGTGGCTCAAAGGGCCCCAGGTACAGCTCGAGCTGCCGCTTCGGAGAGTGCAAGCTATAAGCCTTGGTGGCTTCCACATGGTGTTAAGCCTGCAGGTGCACAGAATGCAAGAGTGAAGGAGGCTTGGCAGCCTCCACCTAGATTTCAGAGGATGTATGGGAAATCCTGGGTGCCCAGGCAGAAGCCTGCTGCAGGGACGGAGCCCTCACAGAGAACCTCTACTAGAGCAGTGCCAAAGGGAAATGTGGGGTTGGAGCCCCCACACAGAGTCCCCACCGGGGCACTGCCTAGTGGAGCTGTGGGAAGGGGGCCACTGTCCTCCAGACCCCAGAATGGTAGAGCCACTGGCAGCGTGCACCGCCAGCCTGGAAAAGCCGCAGGCATCAGACTCCAACCCGTGAGAGCAGCCACGTGGGCTGTGCCCAGCAAAGCCACAGGGGCGGAGCTGCCCAAGGCCTTGGGAGCCCACCCCTCGCACCAGCGTGCCCTGGATGCGAGACACGGAGTCAAAGGAGATTATTTTGGAGCTTTAAGATTTAATGACTGCCCTGCTGGGTTTCGGACTTGCGTGGGGCCTGTAGCCCCTTTCTTTTGGCCCATTTCTCCCTTTTGGAATGGAAATATTTACCCAATGCCTGTACCACCATTGTATCTTGGAAGTAAATAACTTCTTTTTGATTTTACAGGCTCATAGGTGGAAGGAACTTGCCTTGTCTCAGATGAGACTTTGGACTTTGGACTTTTGAGTTAATGCTGGAATGAGTTAAGACTTTGGGGGACTGTTGGGAAGGCATGATTGTATTTTGCAATGTGAGAAGGACGTGAGATTTGGGGGAACCAGGGGCAGAA >MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus GATTTTGGCACTAGGAAGTGGGGTGCTTACGTAACAAATACCTAAAAATGTGGAAGCGACTTTGGAACTGGGTAATAAGTAGAGGCTGGAAGAGTTTTGAGGTACATGYTAGAAAAAGCCTAGATTTCCTTGAAGAGACTGTTGGTAGAAATATGGATATTAAAGGYAATTCTGGTGGGGGGCTCAGAAAGGAAGWGGAGAGCTATAGAGAAAGCTCCCGTCGTCTTAGAGAATACAAAAATCGTCATGAACAGAATGTTGCTAGAAATATGAATGTTAAAGGTGCTTCTGGTGAGGTCTCAGACGGAAATGAGGGAGATGTTATTGAAAATTGGAGGAAAGGTGATCCTTGTTATAAAGTAGCAAAGAACTTGGCTGAATTGTGTTCATGTCCTAGGGCTTTATGGAAAGTAGAACTTGYAAGTGATGAACTKGGATATTCAGCTGAGGAAATTTCTAAGCAAAGTGTTGAGGGCGCAACCTGACTTCTCCTAACTGCTTATAGTAAAATGTGAGAGACATAAAGATGGAANTGTTGAGTAAAAAGGAACCAGAACGTAAAGATTTGGAAAATTCTCAGCCTGATCATGTAACAGAAANNNCAATAGCGTTCTCTGGAAAGAANACCAAGGATGTNNCCGNGCAACCGTTTGCTAAAGAGATTAGRATCGTGACTCGTGGGTCCAATCAACCATCTCAGCARAARCCARGAATAGAGATGRGGTTATTTAGGAAATATCTGTGGAGGACTCTCTTATCTGATGGCTCGAACCCCTATGACTTNCATAGGAGACCGACAAGGNTTTTGAGAATNTTACACCAGCAGAAACACTGCCAACTTGGACTRAAGGGRACAGAGACGAGAAAAAATGAAGGAAGRGTGGCAGACCGAAGGTGGGGCTGTCTCGTTTCAGAGCATGGKGTCACCCCAGCGGGCCCGGAAGATGAATCTCAGGACTCAGAGGCATTAGCCTCCAGCCTTAAGATCTAATGAAGTTTGCCCTGCTGGGTTTTGGACTTGCTTGGGACCNGTGACTCCTTTCTTYYYTTCYATTTCTCCCTTTTGGAATGGGAATATCTATCCTATGCCTGYCCCACCGTTGTATTTTGGAAGCAGATAACTTGTTTNATTRCACAGGTCCACAGATGGAGAGGAATTTTGCCYCTGAATGAATCTYACCCTGAGTYTCTNCCAYACYTGATGYRGRTGATATTTCGCTGAGATTGTGGACTAAGAGTTGGTGCTGGAAGGGGTTAGACATTGGGGAGATGTTGCTATGGGATGCAGGGATTTTGCATGTGAGAAGGACATGATTATGGGGGGAGCGGAGGGCAAAC >MLT2A1 Interspersed repeat MLT2A1- a consensus TGTGATGGTTAATACTGAGTGTCAACTTGATTGGATTGAAGGATACAAAGTATTGATCCTGGGTGTGTCTGTGAGGGTGTTGCCAAAGGAGGTTAACATTGGACTCAGTGGGCTGGGGAGAGGCAGACCCACCCTTAATCTGRGTGGGCGCMATCYAATCAGCTGCCAGCGTGGCTAGAATATAAAGCAGGCAGAAAAATGTGAAAAGAGAGACTGGCCTAGCCTCCCAGCCTACATCTTTCTCCCGTGCTGGATGCTTCCTGCCCTTGAACATCAGACTCCAAGTTCTTCAGTTTTGGGACTCGGACTGGCTCTCCTTGCTCCTCAGCTTGCAGACGGCCTATTGTGGGACCTTGTGATCGTGTGAGTTAATACTTAATAAACTCCCCTTTATATATATCTATTCYATTAGTTCTGTCCCTCTAGAGAACCCTGACTAATACA >MLT2B2 Interspersed repeat MLT2B2 - a consensus TGTGATGGTTAATTTTATGTGTCAACTTGACTGGGCTAARGGGTGCCCAGATAGCTGGTTAAACATTATTTCTGGGTGTGTCTGTGAGGGTGTTTCCAGATGAGATTAGCATTTGAATCAGCGGACTGAGTAAAGAAGATTGCCCTCACCAATGTGGGCGGGCATCATCCAATCCGTTGAGGGCCTGRATAGAACAAAAAGGCAGAGGAAGGGTGAATTTGCTCTCTCTCCTTGAGCTGGGACATCCATCTTCTCCTGCCCTTGGACATTAGAACTCCAGGTTCTCGGGCCTTCGGACTYCGGGACTTGCACCAGCAGCCCCCCAGATTCTCAGGCCTTCGGACTCGGACTGARYYACGCCACCGGCTTCCCTGGTTCTCCAGCTTGCAGACGGCATATCGTGGGACTTCTCAGCCTCCATAATCACGTGAGCCAATTCCCCTAATAAATCYCYTCTATCCATCCTATTGGTTCTGTCTCTCTGGAGAACCCTGACTAATACA >MLT2C2 Interspersed repeat MLT2C2 - a consensus TGTGATAGTTAATTTTATGTGTCAACTTGGCTAGGCTATGGTGTCCAGACGTTTGGTCAAACAYCAGTCTAGATGTTGCTGTGAAGGTATTTTRTAGATGTGATTAACATTTAYAGTCAGTTGACTTTAAGTAAAGGAGATTACCCTCCATAATGTGGGTGGGCCTCATCCAATCAGTTGAAGGCCTTAAGAGCAAAGACTGAGGTTTCCCGAGGAAGAAGAAATTCTGCCTCAAGACTGCAACGTGGAAATCCTGCTGNTTTWCCAGCCTCCAAGCCTTCGGACTCGAACTGCAACATCARCTCTTSCCTGGGTCTCCAGCCTGCCGGCCTACCCTGCAGATTTCGGACTTGCCAGCCTCCACAATCGCGTGAGCCAATTCCTTAAAATAAATCTCTCTCTACACACACCCTATTGGTTCTGTTTCTCTGGAGAACCCTGACTAATACA >MLT2D Interspersed repeat MLT2D - a consensus TGTGATAGTTAATTTTATGTGTCAACTTGGCTAGGCTATGGTGTCCAGACGTTTGGTCAAACATTAGTCTGGGTGTTTCTGTGAAGGTTATTTTTTGGATGAGATTAACATTTAAATCGGTAGACTGAGTAAAGCAGATTACCCTCCCTAATGTGGGTGGACCTCATCTAATCAGTTGAAGGCCTGAATGGAAAAAAAGGGCTGACCCTCCCCCAAGTAAGAGGAAATTCTGCCTGCCTGATNGTCTTCGAACTGGAATATCAGCTCTGCGGATTTTGGACTTGCCAGCCTCCATAATTGCATGAGCCAATTCCTTATAATAAATCTATCTACTCTCTCTACACACACCCTATTGGTTCTGTTTCTCTGGAGAACCCTGACTAATACA >LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2). TGATATGGGAGTGCTGGGAAGGGAAGAGCGTGGTCCCTTTAAATGATACAGAAGGGGGAAAGGAAGTGCTGTGTGGAGGAGGATGTGGTCCCTGGCTAGTACTCCAACCACACAGACCTAGGTTAAGGACAGGCATTTTTGTTGCATTTCCCAAGACCACCCTGGCCTGCCATGCCCCCATCCTGTGCCTATAAAAACCCAAGACCCTAATAAGGCAGAGATAGAAGCAGCTGGACGTCATGAGGAACACATCAGTGGAAGAGCACACAAGCGGCTAGTCATCAAGAGCTCACCAGTGGAAGAGCAGCCCGATAGGCACTGGCACGCAGGCAGGTACCGGCATGCCAGCAGGCCATCGACTGGCAAAATGATGCCGAATTTGGCTGGAGTGGTCAGAGAAGAGCCAGGTCACTGAGCTGCCGGACTCCAGGGGAAAACCATCTCCCTTCTGGCTCCCCCATCCACTGAGAGCTATTTCTACTCAATAAAACCTTGCAGTCATTCTCCAGGCCCACATGTGATCTGATTCTTCTGGTACATCAAGGCAAGAAACCCCAGGATACAGAAAGCCCTCTGTCCTTGTGACAAGGTAGAGGGTCTAACTGAGCTGGTTAACACAAGCTGCCTGTAGATGGCAAACTAAAAGAGCACCTTGTAACACATGCCCACTGGAGCTTCAGGAGCTGTAAACATTCACCCCTAGACACTGCCATGGGATTGGAGCCCCACAGCTTGCCTGTCCAAATGCTCCCCTAGAGGTTTGAGCAGCAGGGCACTGAAGAAGTGAGCCACACCCCCATCACATCCCCTGTGAGGGGAACAAGGGAACCTTTCCTATTTCA >LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat TAAGGAAGGAGACCACCTCTCCCATTGTCTCCTGTTTCATGAGAAAGCAAAAAGTTAAAAAAAGAAGCAGAAGTGAGATCAATGGCCAGATGGTTTAGTGCCAAGAACCAGGCCTGGTAGTTAAACATCAACTCCTGACCTAACCGCTTGTGCTATCCATAGATTCCAGATATTGTATGAGGAAGACTTGTGAAACTTTCTGTTCTGTTCTGCTAGCCCCCATCACTGATGCATGTAGCTCTCAGTCATGTAGCCCCCACTTGCACAATGTATCATGACCCTTTCACGTGGACCCCTCAGAGTTGTAAGCTCTTAAAAGGGACAGGAATCTTTACTTTGGGGAGCTCGGATCTTGAGACGCGAGTCTACCAATGCTCCCAGCTGATTAAAGCCTCTTCCTTCATAGAACCGGTGTCTAAGAGGTTTTGTCTGTGACTGTTCCTGCTACA >LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR GTTGGAGGCCGAAAGAATAGGGTCGTGACCAACTCAGTATACCACTGGAGGCTATATGAGCAAACAGCAAACTGTTCTCATGAATGCAGGATGCTGGCAAGCTGACAACTGCATCTGCCACCAGAAGGAATGCTGAGGGCAGTCATACCCCACGTGCAGTGTTCCTTGTGGTTATCTATAGGAATATCTGGAGGCTGTTGTATAAAGAAAGCAATTATGTGAGCCCGTGATAAATCAAGCAGCTGACCAACCATTACCTCTTCCTCCCTGTTGATTCTACCTAATAAATACAAAGGGCTGTAGAAGCTCAGGGCCCTTGTTCCCTAGAAGAAAGGAGCCGCCTGTCTCCTTCTTTAAAACAGATCTTTTTGTCTTTGTCTTCATTTCTGCATTTGTTCTCTTTTGTTCAGTCCCAAACCGACAGCCATA >LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region GAAGCAGGAAATATAAAAGGAAAAACAAGTAAAGGGAAAACAAGTCCTTTCCTGACCAGTCTGACTCACTCCAAAGTCCTGCTGGAGCTATGATAATTATCTGCAAGGCCAGGCAGGGGCTCCGAAGGAGGGCTCCAGGAGCAGGGATGAGAAAAACAAGTTCTCCTTATCAGTTTCCCTGTTTGAAATTCTCTCCCCATAACATTATTCTTTGTTCTGCTCTCACAACTATTTTTGTAACTATTTCTGCAAGTCTGTAAAGATTTTGTAAGTTCTTGTTTTTCTTTCTGTAGCATGGCAAGGTCACAAGACATGTTTAAGTAAGGTAGGCTCATGTTGCAAATCCTGTTGTAAAACCTGTCACGGTATGATTAACTGCCTTTGTTCTGCTTCTGTAAGACTGCTTTCTCACCTCGCAGGTTTTGCGCCAAAAACCCGACTTGCCCCTGCCTGATGCATGTATAAAAGTCAAGCCCGTCTTTGTTCCGGGCTCAGCCTTTGGATGTTAATCCGCTGGGCCAGTGGCCACCTAAATAAAACCTTCCTGTTGCACCCAGTGATCTCTCCGGCCTCCTGATACCCACAACA >LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18 TGTGGGGAAAAGCAACAGAGGTCAGATTGTTACTGTGTCTGTATAGAAAGAAGTAGACATAGGAGACTCCATTTTGTTCTGTACTAAGAAAAATTATTCTGCCTTGAGATGCTGTTAATCTATGACCTTACCCCCAACCCCGTGCTCTCTGAAACATGTGCTGTGTCAAACTCAGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGATGCTTGAAGGCAGCATGCTCATTAAGAGTCATCACCACTCCCTAATCTCAAGTACCCAGGGACACAAAAACTGCGGAAGGCTGCAGGGGCCTCTGCCTAGGAAAGCCAGGTATTGTCCAAGGTTTCTCCCCATGTGAGAGTCTGAAATATGGCCTCGTGGGAAGGGAAAGACCTGACCGTCCCCCAGCCCGACACCCATAAAGGGTCTGTGCTGAGGAGGATTAGTATAAGAGGAAAGCATGCCTCTTGCAGTTGAGACAAGAGGAAGGCATCTGTTTCCCACCCATCCTTGGGCAATGGAATGTCTCGGTATAAAACCCGATTGTACGTTCCACCTACTGAGATAGGGAGAAACCACCTTAGGGCTGGAGGTGGGACATGCAGGCAGCAATACTGCTTTGTAAAGCATTGAGATGTTTATGTGTATGCATATCTAAAAGCACAGCATTTAATCCTTTACCTTGTCTATGATGCAAAGACCTTTGTTCACGTGTTTGTCTGCTCACCCTCTCCCCACTATTGTCTTGTGACCCTGACACATCTCCCTCTCAGAGAAACACCCACGAATGATCAATAAATACTAAGGGGACTCAGAGGCTGGTGGGATCCTCCATATGCTGAACGTTGGTTCCCCGGGCCCCCTTATTTCTTTCTCTATACTTTGTCTCTGTGTCTTTTTCTTTTCCAAGTCTCTCATTGCACCTTACGAGAAACACCCACAGGTGTGGAGGGGCAACCCACCCCTTCA >LTR6 LTR from human SSAV-related endogenous retroviral LTR-like element TGGCCAGATTCCTGCTTAGATTCATCACTCACATCAAAAAGGCCAACAGAGCCCAACAAGAAACATAGGTCCCCAAGCATAGGCCAGGCCCCTTAAAACTGTGCCTAAGTCGAGTGAAGCCTTTAGATTAATTCTTTTTATTTATCTCTCTTGTTTATTTCCACCTGTTATGCCCTCCGTGCCTTCCTATTCTTCTCACTTCTTTCACGACAGGACGTGTATTTGCAAACACTACTTGGAAGGCAAGAACCTCCAAGAAAGTCTCTTTTGCAGTCGATTTATGTGCTTTGTTCCCAGAGCCTGCCCATAACCTTGAAGAACAATGCAACCTGCCGGTCATAGAAGCGGGGAACATCAACCTTGCTACAGGGTTCGGACACACAGGAAGCCGGACTGGATGTAAAAACTCTAAAAGTGAAAAAAAAAGGACTCCAGAGCATTGACTTTTACCTCTGTCCTAAAAATCACCCTGACTCTAGTTGTCACGATTCTTACCAATTTTTCTGCCCTCACTGGTTTTGTGTGACCCTAGCCA >LTR7 LTR from human endogenous retrovirus RTVL-H2 TGTCAGGCCTCTGAGCCCAAGCTAAGCCATCACATCCCCTGTGACTAGCACATATACGCTCAGATGGCCTGAAGTAACTGAAGAATCACAAAGAAGTGAAAATGCCCTGCCCCACCTTAACTGATGACATTCCACCACAAAAGAAGTGAAAATGGCCGGTCCTTGCCTTAAGTGATGACATTACCTTGTAAGAGTCCTTTTCCTGGCTCATCCTAGCTCAAAAATCTCCCCTACTGAGCACCCTGCGACCCCCACTCCTACCCGCCAAAGAACAACCCCCCTTTGACTGTAATTGTCCTTTACCTACCCAAATCCTATAAAACAGCCCCACCCCTATCTCCCTTTGCTGACTCTCTTTTCGGACTCAGCCCGCCTGCACCCAGGTGATTAAAAGCTTTATTGCTCACACAAAGCCTGTTTGGTGGTCTCTTCACACGGACGCGCATGAAA >LTR8 region TGAAACCGTCTTTGCAAAATTATAACTGAGATAGTGAAAGAGATTTAACTTAACTGCTTTTATCTTGCTTTTAACCTTTAAGCTGTCCTTGTTTATTCCTGGGTGTGGGCTGAACTAACTTTTGGAGAAACTTAGTTTATAGTTTATAGTTTAAACAAAGACAGTAACAGCCCTTTCCCAAGCAGACCTCCTTCATGCCTGGGGACTAGATTGCTTCTGTAGGACTAATGTTAGCGACCAAATTAAAAATTATGGTTTAGGAGTTATGCAGTTTGAGGCTACAAAATTTTAACCCTCTCTAAACTGCTCCTAAGATCAGTGCTTGAGATATTTTGCAGACCCTGCCCTTGACGGATCAGCTGGCACCAACCAGATCAATCAACTGGCTCATCTGATCTTGCGGCCCCCACCCAGGAACTGACTCAGCGCAAAAAGACAGCTTCAACTTCCTATGATTTCATCCCTGACCAATCAACACTCCTGGCTCACTGGCTTCCCCCCACCCACCAAGTTATCCTTAAAAACTCTGATTCCTGAATGCTCAGGGAGACTGATTGGAGTAACAATAAAACTCCAGTCTCCCACACAGACGGCTCTGTGTGAATTACTCTTTCTCCATTGCAATTCCCCTGTCTTGTTGAATCGGCTCTGTCCAGGTAGTGTGCAAGGTGAACCCCTTGGGTGGTTACA >LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) AAAGAATAAATCACTGATATAAGAGTTAAGAAAAAATCACTTAGGCAGAGAGCAAGGGTATGGGAGTCCTCAGTAAGGCTTTTATTTTTAATGAAAAGCAGCCCCAAATCATTTTCTAACAAAAAGCACCCTGCAAGCTGGAAGTTTGCACAGGTGGATGCTGGCAGGAACTAAGGATTAGATATGTTCAAGATGGTGGTTCCATCTTCCCTTCTTTGCCAGCCAGGTGTACTGTAAAGGAGCAGACAAGATGGCGCTAATCAACTGGAAAGCCCAGTTGCATAAAAAGATTAGGCTTCCCTGCGCACCATGCAAACGTCATATCTGATCAAACCAATCTATGAGCTCTATGTAAATCAGACACCACTTCCTCAAACTGGACTATAAAACTCGATGGATTCACCACCAGCTGGTCTTTTCCGCTTGGAGACCCCTTCCTCTATAGAGGAAGCTGTTTCTCTTTGTCTTCTCTCCTACCCATTAAACCTCTGCTCCTAAACTCCTCATGTGTGTCCATATCCTAAATTTTCCTGGCACGTGACAACAAACCCCAGTGTATACACACCCCAGACAACATAGCCACTTCAAAATGGGGGCTCGTCTGGGAT >LTR10 Human DNA sequence 5'-flanking minisatellite PMS32 TGTTGTAAATAAATTTTTGGTGCTGCAAAAGAAATACCACTCAAACATAAGTTTAATTTTCTCAGCAAGGCAATTTTACTTCTCTAGAAGGGTGCGACTCGCAGATGGAGCAATGGCCAGAGCACACCTGAACAAGGGAGGGGAAGGGGTTCTGATTCCTGACACAGGTAGCCCCTACTGATGCGTCGTTCCCGTATCGGCTAGGGTTGGACTGCACAGTCTAAGCTAATTCCGATTGGCTACTTTAAAGAGAGCAGGGGTATGAGCCAGAGTGGCGGGGTGAGTAGTTTGGTGGGAAGGGTGGTT >LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1) TGTGTTTCTGCAGGATGTACAGCCCTCCCAGCACGGTGCTCGCTTCCCTAATGCCATTCCACAGTATTTGTTGCAGAGAAGGAAGGCAGCATGCCAGGACAGATGGAGACAGGACTAATTTGGCCTGAGGTATGTAATTTTGAACTTGAGCCTCTCTCTGGGACTGTAAAACTCCAAATCAAAGCTAATCTGAGAATACATACATCTGAAAGATGATTAGGACTGTAAACATCTATTAATATTCAACTCTGATACAAAGTACAAGTTGTTTATTCTTACCACGCAAGGCCAAAAAAGGGGAGAAAAAAAAAAAAGCACACAGCATATGCACTGGAAAGTTTCGCTTATTCAAAACAGTATTTGTCAAGCACCTCCAGTCTGGTGCTGCAGGGGAAACAAAGATTAAACAGCCAGGCGGACACTGCTCTGCTTCCAAGGTGCTTACGGTCTTAAGAAGGAGACAAGACATGTTTATAAATAGCCAAAATGCAACCCAGAAAAGGCTAAAAAACACTGAGAGGGAGGGAGAAATAAACGAAGCAAGAGGTCTCCGGAGGAAGAGATGAATGAATTAGCCTATTAATAACTCCGTCACTGTAATCCCAATGTAAAGCAAGAATTCCAAACCAGGAAAGGTCAAACTGAAGTATTTGAGGAACACAGGCGTCGCCTAAGCCCTTCACA >LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1) TGTATCTAGTTAATCTGGTGGGGAGTTGGAGAATCTTTATGTCTAGCTAAGGGATTGTAAATACACCAATCAGCACTCTGTATCTAGCTCAAGGTTTGTAAACACACCAATCAGAACCCTGTGTCTAGCTCAGGGTTTGTGAATGCACCAATCAGCACTCTGTATCTAGTTAATCTGGTGGGGACTTGGAGAATCTTTATGTCTAGCTAAGGGATTGTGAATGCACCAATCGGCACTCTGTATCTAGCTCAAGGTTGTAAATGGACCAATCAGCACTCTATGTCTAGCTCAGTGTTTGTAAATACACCAATCGACACTCTATCTAGCTAATCTAGTGGGGACGTGGAGAACTTTTGTGTCTAACTCAGGGATGTAAATGCACCAATCAGAACCCTGTCAAAATGGACCAATAGCTCTCTGTAAAACAGACTGACTTTCTGTAAAATGGACCAATCAGCAGGATGTGGGTGGGGCCAGATAAGAGAAGAAAAGCAGGCTGCCTGAGCCAGCAGTGGCAACCCGCTTGGGTCCCCTTCCACACTGTGGAAGCTTTGTTCTTTCGCTCTTTGCAATAAATCTTGCTGCTGCTCACTCTTTGGGTCCACACTGCCTTTATGAGCTGTAACACTCACCGCGAAGGTCTGCAGCTTCATCTGAAGCAGCGAGACACGAACCCACCAGGAGGAACAAACAACTCCGATGCGCTGCCTTAAGAGCTGTAACACCGCGAGGTCTCGAGCTTCACTCCTGAGCCAGCAAGACCACAAACCCACCAGAAGGAAGAAACTCAGGAAAACAGCCGAACATCAGAAGGAATAAACTCTGGACACACCACCTTTAAGAACTGTGACACTCGCTGCGAGCGTCTGTGGCTTCA >LTR13 LTR from a putative human endogenous retrovirus TGTGGGCCGCAAGCCAGGGAGCTGCCCAGGCAAGAGCCTGAAGGCACAAGCTGTTCCAGTACAGCAAAGAAAATAATTAGAATAAGAAAAGTTTTACTAGAGATAGGAAACGGATAGGATTATATCTGACTATTATTAATCATTAGTTTGTAGCATCACTCTTTGTTCTATTACCATAATGATCTCTGTTCTATTATGATTACCTTGGGGGAAACCAGGCCACACAGAGTTAGGAGCTGAAGGGCCACAGTGAGAGGTGACCAGAAGACGAGAGTGTGAGCCCTCATTCACGCCCAGAGAAGGGCCGCTGGAGGGCTCCTTGGCCTAGCGGTAATGCCAGTGCCTGGGAAGGCCCTGGTTACTTAGCAGGCCTTGGTCTAGCGGTGGCCCCAGTGCCTGGGAAGGCACCCGTTACTTAGCAGACCCGGAAAGGAATCTCCCTCTCTCCAGGGGAGACAGAGAACGCTCCGCTCCACCACCTCTTGTGGGAGGTCTGACATTAGCCAGGCCGGGCCGAGTCATCCGGAGGCTCCAACGTCTGTCTCCCTGTGATGCTGTGCTTCAGTGGTCACGCTCCTTGTTCACTTTCATGTTCAGCCTGTGCACCTGGCTCCTCCTTTTAAGTTCTTAGAAGACAGCAGTAGCAGAACTAGTAGGAGTACCACAGTCTTCGATCTTTCTGATAAGTGCATAGAAGAAACGCTGACGTTTGCTGTCCTCCCTCTCCACCTCGGCTACCACAAAGGGAAAGGCCCCCTGTCCAGTGGACACGTGACTCGCGTGACCTATCGATCATTGGAGATGACTGGCACTCCTTACCCTGCCCCCTTGCCTTGACTACAATAAATAGCAGCGCCTCCAGGCACTCGGGGCCACTACCTGTCTGTCTCCGCGCTTTGGTGGCAGTGGTCCCCCGGGCCCAGCTGTCTTTCTTCCTATCTCTTTGTCTTCTGTCTTTATCTCTTCGATCTCTCGTCTCCGCACACACGCGAAGAGAAAACCCACAGACCCGGTAGGGGTGGACCCTACA >MER1A Nonautonomous DNA transposon CAGGGGTCCCCAACCCCCGGGCCACGGACCGGTACCGGTCCGTGGCCTGTTAGGAACCGGGCTGCACAGCAGGAGGTGAGCGGCGGGCGAGTGAGCGAAGCTTCATCTGTAKTTACAGCCGCTCCCCATCACTCGCATTACCGCCTGAGCTCCACCTCCTGTCAGATCAGCGGTGGCACTAGATTCTCATAGGAGCGCGAACCCTATTGTGAACTGCGCATGCGAGGGATCTAGGTTGCGCGCTCCTTATGAGAATCTAATGCCTGATGATCTGTCGCTGTCTCCCATCACCCCCAGATGGGACCGTCTAGTTGCAGGAAAACAAGCTCAGGGCTCCCACTGATTCTACATTATGGTGAGTTGTRTAATTATTTCATTATATATTACAATGTAATAATAATAGAAATAAAGTGCACAATAAATGTAATGCACTTGAATCATCCCGAAACCATCCCCCCCCCCTGGTCCGTGGAAAAATTGTCTTCCACGAAACCGGTCCCTGGTGCCAAAAAGGTTGGGGACCACTG >MER1B Nonautonomous DNA transposon CAGGGGTCCCCAACCCCCGGGCCGCGGACCGGTACCGGTCCGTGGCCTGTTAGGAACYGGGCTGCACAGCAGGAGGTGAGCGGCGGGCGAGTGAGCATTACCGCCTGAGCTCCGCCTCCTGTCAGATCAGCGGCGGCATTAGATTCTCATAGGAGCGCGAACCCTATTGTGAACTGCGCATGCGAGGGATCTAGGTTGCGCACTCCTTATGAGAATCTAATGCCTGATGATCTGAGGTGGAACAGTTTCATCCCGAAACCATCCCCGCCCCCCGGTCCGTGGAAAAATTGTCTTCCACGAAACCGGTCCCTGGTGCCAAAAAGGTTGGGGACCACTG >MER2 Nonautonomous DNA transposon CAGTCGYCCCTCCGTATCCGTGYGTTCCACATCCGTGGATTCAACCAACCGCGGATCGAAAATATTCAGGYAAAAAATTGYATGGTTGCGTCTGTACTGAACATGTACAGACTTTTTTNCTTGTCATTATTCCCTAAACAATACAGTATAACAACTATTTACATAGCATTTACATTGTATTAGGTATTATAAGTAATCTAGAGATGATTTAAAGTATACGGGAGGATGTGCGTAGGTTATATGCAAATACTACGCCATTTTATATCAGGGACTTGAGCATCCGCGGATTTTGGTATCTGCGGGGGGTCCTGGAACCAATCCCCCACGGATACCGAGGGATYACTG >MER3 Nonautonomous DNA transposon CAGCGCTGTCCAATAGAACTTTCTGTGGTGATGGAAATGTTCTATATCTGCGCTGTCCAATACGGTAGCCACTAGCCACATGTGGCTAYTGAGCACTTGAAATGTGGYTAGTGCGACTGAGGAACTGAATTTTTAATTTTATTTAATTTTAATTAATTTAAATTTAAATAGCCACATGTGGCTAGTGGCTACCATATTGGACAGCGCAG >MER4A a consensus TGTGAAAGGAAAATAAATCTCGGGRCCCCMAAATCACTAAGCCAAAGGGAAAAGTCAAGCTGGGAACTGCGTYAGGCAAACCTGCCTCCCATTTTATTCCTAAATAAGATAGCTACAAAGATAAAAGGCTACATACCTCTCTCACAATTTYCYACAAGGAAATTCCTTGCGGACCTCAAGATCTTTACCCTAAAACAGTTCTGYTGAMYTTCACCTTGGCAWTGYAAATGGRTACAGGACAAAGGTACAGAACTGAAAGTCATCCCTCTGCTCACCTGAGACAAATGCATATCTGATTGCTTCCTCTGCCCTATTGTTTATGTAAAAATGCAGATTCACTGAGCCAGACTMAATTGTGTATTCAGTGAAARGCTGATCARRGACTCAAAAGAATGMAGCCWTTTGTCTCTTATCTACCTATGACCTGGAAGCCCCTRCTTCGAGTTGTCCCGCCTTTCCAGACCGAACCAATGTACATCTTACATATATTGATTGATGTCTCATGTCTCCCTAAAATGTATAAAASCAAGCTGTRCCCCGACCACCTTGGGCACATGTCGTCAGGACCTCCTGAGGCTGTGTCACGGGTGCGTCCTTAACCTTGGCAAAATAAACTTTCTAAATTGAYTGAGACCTGTCTCAGATACTTTTGGGTTCACA >MER4B a consensus TGTAAACCAAAAATAAAATTCTAAGKCCCCCAACCGTCTGAATGGACYCCTCCTCTYGGCCAAGGGCAYTCCAAAGYTAACCTGRAAAACTAGTTCAGGCCATGATGGGAAGTGGGGGTCGGACACGCCTCATTATACCYTCCYYCTTCTGGAATTCAGGCACAACTGACCAGCATTAACATCAACACAGACNTTAAGTCTGATAAGAAACAGTTTACAAYCTRTTCTCTCTGAAGCCTGCTANCTGAARGCTTCATCTGCACGATAAAACTTGGTCTCCGCAACCCCTTATNTCATAACCCGGACATTCCTTTCCATTGATCTYAANTCTTCAACCARTTGCCAATCAGAAAATCTTTGAATCTACCTATGACCTGGAAGCCCCCGCTTCGAGTTGTCCCGCCTTTCCGGACCGAACCAATGTACATCTTACATGTATTTGATTGATGCCTCATGTCTCCCTAAAATGTATAAAASCAAGCTGTRCCCCGACCACCTTGGGCACATGTKGTCAGGACCTCCTGAGGCTGTGTCACGGGTGCGTCCTTAACCTTGGCAAAATAAACTTTCTAAATTGAYTGAGACCTGTCTCAGATACTTTTGGGTTCACA >MER4C a consensus GTTRGNGTTRGNGATTGCNNNTGTGAAAGGAAAATAAAAACTTGGGACCCNAATTCACTNTGCCAAAAGGAAAAAATTAAGCTGAAANCTGAGTCATGCAAGAAACTGCCTTTCCTTTTNTTCCTAAGCAGATANCTACAGATAAAAGGTTAAATATCTCCACAGRCAGTGACTCTATGTTCACCTTYCYTATGTAAAGTGKCGATTTACTGAGCACGMKAKGRATAGATAATTGACTATTCCCCTACCTGCTCCTTTTCTCTTGCAACATGTGGATTACCAYAMCCTYYYTCTTTCCCCTCCAGCCTACYTTTCCCNTTTAAATATTGAAGCCCTCAAANTCATCNCTGGAGAAAGGCACAGACCACAGAMTSTTTYTGTRAYTYCGTGTTTCTCCTNGGCATGTCCTTAACNTTGGCAAAATAAACTNCTAAATCGATTGAGACCTGTYTCAGAYACWTTTKGGTTTACA >MER5a a consensus CAGTGGTTCTCAAAGTGTGGTCCCCGGACCAGCAGCATCAGCATCACCTGGGAACTTGTTAGAAATGCAGATTCTCGGGCCCCACCCCAGACCTACTGAATCAGAAACTCTGGGRGTGGGGCCCAGCAATCTGTGTTTTAACAAGCYCTCCAGGTGATTCTGATGCATGCTCAAGTTTGAGAACCACTG >MER5b a consensus CAGTGGTTCTCAACCYTGGCTGCAYATTAGAATCACCTGGGGAGYTTTTAAAAATCCNGATGCCCGGGCCACACCCCAGACCAATTAAATCAGAATCTCTGGRGGTGGGACCCAGGCATCAGTATTTTTTAAARCTCYYCAGGTGATTCCAATGTGCAGCCAAGGTTGAGAACCACTG >MER6 Nonautonomous DNA transposon CAGCAGGTCCTCGAATAACGCCGTTTCATTCAACGTCGTTTCGTTATAACGYTGATGAGRAAAAAAATCGATTCCCGGCCGGGGCCACTGTCTGTGTGGAGTTTGCACGTTCTCCCCATGTCTGCGTGGGTTTTCTCCAGGTACTCCGGTTTCCTYCCACATCCCAAAGATGTGCACGTTAGGTTCAYTGGCGTGTCTACATGGTCCCAGTCTGAGTGAGTGTGNNTGTGTGTGTGAGTGTGCCCTGCGATGGGATGGCGTCCTGTCCAGGGNTGGYTCCCGCCTTGCGCCCTGAGCTGCCGGGATAGGCTCTGGCCACCCGTGACCCTGAAMTGGAATAAGTGNRTTGRAAAATGAATRAATGAATGARTACGAATTATTATAAAATAAAAATTTGTAAAGTCTATGNTAATCTTACACATACATGACAATACATGAAATGGTAAGAAAGTGATCAGTGAGCCTGCCATATATGTTATTGCTTGTTTCTGAACTGAGTGGTGGGAGGAGGTGTTCCTAACAATGTTCACTTTGCAGACATTTATTTCTTTATTTAATCCATTATCACTATGACCACTGTCATTCAGTKATTCWCCAAAAATTGGGTAAATAATTATCWTRYTTGTTTTTATTAATCTTTCTTAAATGTATGTATAGCTCACATTTATTTCAGTGTTTAATATTAGAAGTGTTTTGGGTCTTTATTTAGAAGTTTGGTGATGTTTTTGTGACCAGAAATATGCCATAGGAACTTAACTCTTGTTTATATCAATTAGCCTRTGGTAAAATTGGTTTCGTTATACGTCGTTTCACTTAAAGTCGCAGTTTCCAAGAACCTATCAACGACGTTAAGTGAGGACTTACTG >MER7A Nonautonomous DNA transposon CAGTCATGCGTCGCTTAACGACGGGGATACGTTCTGAGAAATGCGTCATTAGGCAATTTCGTTGTGCGAACATCATAGAGTGTACTTACACAAACCTAGATGGTATAGCCTACTACACACCTAGGCTATRTGGTATAGCCTATTGCTCCTAGGCTACAAACCTGTACAGCATGTTACTGTACTGAATACTGTAGGCAATTGTAACACAATGGTAAGTATTTGTGTATCTAAACATAGAAAAGGTACAGTAAAAATACGGTATTATAATCTTATGGGACCACCGTCGTATATGTGGTCCATCGTTGACCGAAACGTCGTTATGCAGCGCATGACTG >MER7B Nonautonomous DNA transposon CAGTCATGCGTCGCTTAACGACGGGGATACGTTCTGAGAAATGCGTCATTAGGCAATTTCGTTGTGCGAACATCATAGAGTGTACTTACACAAACCTAGATGGTATAGCCTACTACACACCTAGGCTATRTGGTATAGCCTATTGCTCCTAGGCTACAAACCTGTACAGCATGTTACTGTACTGAATACTGTAGGCAATTGTAACACAATGGTAAGTATTTGTGTATCTAAACATAGAAAAGGTACAGTAAAAATACGGTATAAAAGATAAAAAATGGTACACCTGTATAGRGCACTTACCATGAATGGAGCTTGCAGGACTGGAAGTTGCTCTGGGTGAGTCAGTGAGTGAGTGGTGAGTGAATGTGAAGGCCTAGGACATTACTGTACACTACTGTAGACTTTATAAACACTGTACACTTAGGCTATACTAAWTTTATWWWWWWWWWWWWWYTTTTTCAGCAATAAATTAACCATAGCTTACTGTAACTTTTTTACTTTATAAACTTTTGCATTTTTAACTTTTGACTCTTTTGTAATAACACTTAGCTTAAAACACAAACACATTGTACAGCTGTACAAAAATATTTTCTTTCTTTATATCCTTATTCTATAAGCTTTTTTCTATTTWWAAATTTTTWATTTTTTTATTTTTTAAAANATTTTTGTTAAAAACTAAGACACAAACACACACATTAGCCTAGGCCTACACAGGGTCAGGATCATYAATATCACTGTCTTCCGCCTCCACATTTTGTCCCACTGGAAGGTCTTCAGGGGCAATAACATGCATGGAGCTGTCATCTCCTATGATAACGATGCYTTCTTCTGGAATATCTCCTGAAGGACCTGCCTGAGNCTGTTTTACAGTTAACTTTTTTTTTTAATAAGTAGAAGGAGTAYACTCTAAAATAATAATAAAAAGTATAGTATAGTAAATACATAAACCAGTAACATAGTCATTTATTATCACTATCAAGTATTATGTACTGTACATAATTGTATGTGCTATACTTTTATACAACTGGCAGCGCAGTAGGTTTGTTTACACCAGCATCGCCACAAACGCGTGAGTAMTGAGTRATATGTTGCACTACAACGTCACTAGGCGATAGGAATTTTTCAGCTCCATTATAATCTTATGGGACCACCGTCGTATATGTGGTCCATCGTTGACCGAAACGTCGTTATGCAGCGCATGACTG >MER8 Nonautonomous DNA transposon CAGTTGTCCCTCTGTATANNCGGGGGATTGGTTCCAGGACCCYTGTGTATACMAAAATCCGCGCATACTCAAGTCCCGAAGTCGGCCCTGCGGAACCCACGNATATGAAAAGTCGGCCCTCCATATATACGGGTTTCGCATCCCGCGAATACTGTATTTTCAATCCGCGTTTGATTGAAAAAAATCCGCGTATAAGTGGACCCACGCAGTTCAAACCCGTGTTGTTCAAGGGTCAACTG >MER9 a consensus TGCGAGACCAGCTCGACTGGGGACAGCCTAACCCAGTGGCACTAGAGGAATTAAAGACACACACACAGAAATATAGAGGTGTAAAGTGGGAAATCAGGGCCTCACAGCCTTCAGAGCTGAGAGCCCCAAACAGAGATTACCCATGTATTTATTAACAGCAAGCCAGTCATTAGCATTGTTTCTATAGATACTCGATTAACTAAAAGTATCCCTTATGGGAAAAGAAGGGATGGGCCGAAATGAAGGGGTGGGTCTGGCTAGTTATCTGCAGCAGGAACATGCCCTTAAGGCACAGATTGCTCATGCTATTGTTCATGGTTTAAGAACGCCTTTAAGCGGTTTTCCGACCTGGGTGGGCCAGGTGTTCCTTGTCCTCATTCCAGTAAACCCACAACCTTCCAGCGTGGGCGTTANGGCCATCATGAACATGTCACAGTGCTGCAGAGATTTTGTTTATGGCCAGTTTTGGGGCCAGTTTATGGCCAGATTTGGGGGGGCCTGTTCCCAACA >MER11A a consensus AGGTGTTGCGGGAAGTCAGGGACCCCGAATGRAGGGACCGGCTGRAGCCATGGCAGARGAACGTRRATTGTGAAGATTTCATGGACATTTATCGGTTCCCAAATTAATACTTTTATAATTTCTTATGCCTGTCTTTACTTTAATCTCTTAATCCTGTTATCTTCGTAAGCTGAGGATGTATGTCACCTCAGGACCACTGTGATAATTGTGTTAACTGTACAAATTGATTGTAAAACGTGTGTTTGAACAATATGAAATCAGTGCACCTTGAAAAAGAACAGAATAACAGCRATTTTTAGGGAACAAGGGAAGACAACCATAAGGTCTGACTGCCTGCAGGGTCGGGCAAAAAGAGCCATATTTTTCTTCTTGCAGAGAGCCTATAAATGGACATGCAAGTAGGGAAGATATCGCTAAATTCTTTTCCTAGCAAGGAATATTAATATTAATACCCTGGGAAAGGAATGCATTCCTGGGGGGAGGTCTATAAACGGCCGCTCTGGGAATGTCTGTCTTATGTGGTTGAGATAAGGACTGAGATACGCCCTGGTCTCCTGCAGTACCCTCAGGCTTACTAGGGTGGGGAAAAACTCCACCCTGGTAAATTTGTGGTCAGACCGGTTCTCTGCTCTCGAACCCTGTTTTCTGTTGTTTAAGATGTTTATCAAGATAATATGTGCACCGCTGAACATAGACCCTTATCAGTAGTTCTGTTTTTGCCCTTTGCCTTGTGATCTTTGTTGGACCCTTATCAGTGGTTCTGCTTTTGCCCTTTGTCCTGTTCCCTCAGAAGCATGTGATCTTTGTTAGACCCTTATTAGTAGTTCTGCTTTTTGCCCTTTGAAGCATGTGATCTTTGTACCCACTCCCTGTTCTTACACCCCCTCCCCTTTTGAAACCCTTAATAAAAAACTTGCTGGTTTGAGGCTCAGGTGGGCATCACAGTCCTACCGATATGTGATGTCACCCCCGGCGGCCCAGCTGTAAAATTCCTCTCTTTATACTGTCTCTCTTTATTTCTCAGCCGGCCGACACTTAGGGAAAATAGAAAGAACCTACGTTGAAATATTGGGGGCGGGTTCCCCCGATACTAATTNTCTT >MER11B a consensus TGCAAGTAGGGAAGATATCGCTAAATTCTTTTCCCAGCAAGGAATATTAATAATTAATAGCCCTGGGAAAAGAATGCATTCCTAGGGGGAGGTCTCTRAAATGGCCGCTCTGGGAATGTCTGTCTTATGTGGTTGTAGATAAGGGATGAAATACGCCCTGGTCTCCTGCAGCACCCCCAGGCTTACTAGGATGGTGGGAAATTCCAGCCTGGTAAATTTTAGTCAGACCGGTTCTCTGCTCTCGAACCCTGTCTCCTGTTAAGATGTTATCAATGATAATGCGTGCCCGAAACCTCATTAGCAATTTTAATTTCGCCTCCATCCCTGTGGTCCTGTGATCTCGCCCTGCCTCCATTTGCCTTGTGATATTTTATTGCCCTGTGAAGTATGTGATCTCTGTGACCCACACCCTGTTCGTACACTCCCTCCCCTTTTAAAATCCCTAATAAAAACTTGCTGGTTTGACGGCTCGGGGGGCATCACGGAACCTACCGACATGTGATGTCTCCCCCGGACGCCCAGCTTTAAAATTTCTCTCTTTTGTACTCTKTCCCTTTATTTCTCAGRCCGGCCGACACTTAGGGAAAATAGAAAAGAACCTACGTGAAATATTGGGGCTGGTTCCCCCGATACTAATTNTCTT >MER20 Nonautonomous DNA transposon CAGTGGTTCTCAACCGGGGGTGATTTTGCCCCCCAGGGGACATTTGGCAATGTCTGGAGACATTTTTGGTTGTCACAACTGGGGGGGGGGRATGCTACTGGCATCTAGTGGGTAGAGGCCAGGGATGCTGCTAAACATCCTACAATGCACAGGACAGCCCCCACAACAAAGAATTATCCGGCCCAAAATGTCGATAGTGCCAAGGTTGAGAAACCCTG >MER21 a consensus GCCCTYCTRTTKCAGRAAAACCTCTCTCAAACCATGTTTTYCCTCTGCTSTCATACCACGACAACAGTCGTCAACACAGAAGACTTCTGTGACCAAATGTGTGGAGGATTTTCCCCACACACCAAGCAGCAGACACCAGCTGGGCGTCCTCCAAYTCAGTTCCGACACTGTCTACCTGGAGATAGCGTCAGATCCCACAGGTTGAGGTCTCAGTCCCCAAGACTGNCCCCACCTTCAGAYACAGTTGCAAGYCCGGGYCTCTGGAACTTCTGACCAACTGGCTTCAARTTGGGGTTCCCACGACACCTCTTTAGGTTCGAYTAATTTGCTAGAGTGGYTCACAAAACTCARRGAAACACTTATTAWGTTTACTGGTTTATTAATAGATGATATTTCAAAGGATACAGATGAAGAGATACATACGGGGCGGTCTGGAAGRKCCCAGCGCAGGAGCTTCCGTCYTGATAGRRTTGGGGTTACACCCTCCAGATTCTTCACCTTCTGTYAGCCTCCACGTGTTCAGCTCTCCAGAAGCTCYCTGAACCCTGTCCTTTGGGCCTTTTATGGAGAYCTCATTGGNTGTCCATGACTGAAGCATGGACGAYTGNNAWAATGTGATTGGRCAAAAAGGGTCTGATCTAATCCCAGCAAGGYCTGTCCAGATTCTTCTTGGTCTCTTTGTGCAGCATTCCTTCCTCCAGGGTATGGGGCAGGACCYCCTCTGGAATGAGGGTCTTACGACCCACAATCAGATTATAGAGTCCTGCCTTGGGCGGMTGAAARGAGGRCARGAGAAGGYCAGAGARATATTCTGTTTCCTGAGGCCTAAAGCACCTCTACATTATAGCGAAAGACTGTAACCATGGTCATGTGAGTTATGGACAAGAACCATGGACAAAAACACATATACAATCATATCCCACCTCCCAACGC >MER21B a consensus TCAACACACAACACTTCTGTCACCAAACGTGTGGRRRTTTTTCCCCACACACAAACAAKTCTTCAGTTCTGCGGCGGACACCARCTGGGTGTCCTCCAATTCARTTCAGTTCTGACACTAWCTACCTRGAGATAGCGTCAGATCCCACAGGTTTAAGGGCTCAGTCCCACAAGACTGCCCCCACTTCAGATRCCAGTCGCAAGTCTRGGTTKTCACCCGTACTTCTGACCAACTGGCTATAAATTGTTCCCACGACCCCTCTTTAGGTTCGATTAATTTGCTAGAATRGCTCACARAACTCAGGGAAACACTTATATTTAYCGGTTTATTATAAAGGATATTACAAAGGATACAGATGAACAACCAGATGAAGAGATRCATAGGGCGAGGTMTGGGAGAGTCCNGGGNNCAGGAGCTTCCGTGCCCTCTCTGGSTNNRCCACCTTCCWGGCACCTCCACGTGTTCACCAACCCGGAAGCTCTCCGAACCCTGTCCTTTTGGGTTTTTATGGAGGCTTCATTACGTAGGCATGAYTGATTACATCANTGGCCATTGATTATCAACTCAACCTYCAGCYCCTCTCCYCTCCCCAGAGGTTGGAGGGTGGGGCTGAAAGTTCCAACCYTCTAATCTGCCTTGGTCTTTCTRGCGACCAGCYCCCATCCWGRAGCNTNCTAGGGGCTGCCCRCCAKGAGTCGMCTCATTAGMACAAAAGRNCRTYNCTATTACCCAGGARATTCCAAGGGTTTTAGGAGYTCTGTGTCAGGAACCGGGGTCAAAGACCAAATATTAGAT >MER22 Human SSTI moderately repetitive DNA sequence family GAGCTCTGGGCTTCCATACCTGTGTGGGACAGGGAAGCTCTCTCGGTCTCCATGGCCCAAGTGATGGCTGCACGCTCGGTCCAGGAAGAGGCGGAGGAAGCCCACCGCTCCTGACATTGGCCTTCTAGGAAAGGCGGTGTTGCATCCCACCTGCACTTCCTCTCTGATTCTTGAGGGCCAACCGCTTCCTCCGCTCCTGGGGAAAGTGCCTTCTAGCACCGAATCTTTTGGCTGCCACGGATGTCAGGGAGCCAACGGGACTGGGTTTTGGCTGGGTGCAGGGGAGGTTGCGTCAGGGGTACTAGCCGGCGGCGGGCTGGGGGTGGGGTGTACTTTGTCCAAACTCCCGGCTCCTCTGGCGGGCCTCCCTGAACGTGGCGTGGACTCGCGCACAGGCCCTGTCTCGCAGGTTTTCAGGTGCGCTTGGCTTTTCCTCCGCTTTGTGGGGCAGGTCTCCAGTGCCCCCGGCGCACGCCTGGACATCACTGTCCGTCTCGTCGTCGCCCCTACGGCCTCAAAGACACACGCTGCCTGCATGTGCTCTTGGGGGACGACAGTGCACATGTGGACACACTGGCTCCAGCTCGGACTCGCCTCTGTCTCTCTTTGCCCGTGTCGCCGGAAGCCGCCTCGGGTTGCCGGAGCCCTCGGGCCTTGGAGATGAAGGCAGGCCCCTGCTCCTGCCAGGAAGGAGGGAGGCAGTGGGCTCATGGGTCGGTGCCTTTGCAGCCGACAGCACGTGCGGCCCTGGGGATCTTCCTGTGCCCCGGCGAGACCCTTTCCGCCTCACTGCATTGGAACCCCATTCCCGATCACCCGCTGGGATCCATCATCGGACTCCAAGAGGAGTCCGCGCAGCCAGCCGGCACCCCGAAGCTCCTCCTTCAGCGGGAACCGAAGCAGAAGAGCGATCAAGGAGGTCCTCACCACAGGACTCCTATGGGTCCGACCCTGGGTCTCCCGCAGGCCCCTCTGGCAGTCCTCTTCCCACCCGCCGCCTCGGCTTCGCCGCCGCCGCCGCAACCTCCAGCACCGCCCCCCAGGCCCCGCAGCCGCCGTCGCCGCCATTTTTTAAAGGGTCGCAGCCTGACTCTGCGGAGTAAGGGGGGGTGGAGCGGGGGAGTCGCTCGCCAGCATGCGCGAGCCCGAGCCGCCGCTTGGGTCACAGTGAAAGCCACCGTTGCCCGGGGATGGGTCCCTGACACTTGGGGAAGTAGGAGCCCTGTGTGATCGTGCGTCTGAGTCTGGGCTGAGACCAGTCCTGGCCAGGGCAGTTACCAGGACGGTCTCGGAGGCCGGGATTCGCGGAGGGTCCAGCAGCAGGAAGAAACCCCAGGAGGAAGAAACCTCAGACAGATCGCCGGCGAGGCAGCGCGGGATCCCAGCCTCAGGCGTGCGCGGACGGTGTGCGGGTGAGTCTCCCCAAAAGTGGAGCCCTTGTGATGACGAGCACAGGTCCGCCTGCGTGCCCGTGGGCGGCTCTCTCACCGGTGGCTCTCAGTCGCGGAGAGCAGAACCCGCAGCTTCAGGGGCTGCTGCGGGAGGGTGTTCCCTGCTGTACGT >MER25 Older LINE1 5' end region - a consensus ACAAANTAGGGAAAAATGGCGGATAGGAGGCAGGACTAAATTGCAGCTCCCACTCGGATGGACAGAGCAGCATGTGGAGACTCACATCGTGAACTTTTGCTCCAAGAACCACCGCAGGAATATAYCAAGAAAGCCGAGAGAATYCACAGACCCTCTGAAGGAAGCAGATTGCTCCTGCARGAYCCAGGAAACGGCCCAAAAACTGTGAGTGCCCAAAGTGTGAGAAGGGRAAAATCTGCCCCCGAACACACATCCTYACTGGGGAACCTGAAGATCCAGATCACAGGAGAAGGATTTGACCTTACCTGGAGCTGAGACAATTTAGAGGGCCGAGCAAAATACAGGGGTAGAGGAAGCAGCGGGAAAAGCCCCGTGGGCACTCCCAGTCYYYAGNNAARCCCAGGGAAGCCATTTCTGACTTATCTCACAGGGGTCCCTTGGGAGGGCAGCCGGAGGAACTGGGAAAGGGSCACAGGGAAAAGAAAACCTCCAGCTGAACTTTGTAATAATTCCGACCGAKAGAGAAATTTCCTGGCCAGAACTMAGGGCAGGGCGRCGKAAGTGCTGGTGGGAGACCAGAAGTGCTGTTGGGGGGGACACGGTRGGAGTGAGACYTGCCTTTTGAGCTGCATGGGAGCTGGGTGAGGCCTGTNACTGCCAGCTTTCCCCCACTTYCCTGGTGACCTGCATGACACAGAAGAGGCAGCCATAATCCCCCTGGGAACGTAACTCCATTRGCCTGGGAACTACACCCCCATCCCTCACAGTAGCCGCAGCAAGCCCCGCCCAAGGAGACTCTGAGCTCAGACATGCCTAACCCTGCCCCCACCTGATGGTCTTTCTCTACCCACCCTGGTAGCCGAAGACAAAAGACATAAWCTCTTGGGAGCTCTAYGGCCCCACCCATCGCCTGAGAAACWGAAATAYTTAYCYWGGCMAMCTTAGGGCAAGCTTRTATCCWCCCTATACTRTCGCAGCTGATGCCCTCTTGAAAGCGCCACCTCTTGGCTGGAGGCCAACCAACTCAAGACATTACAGCAAYTCAYRACAGAATAACCCTGCCCCTARRRAAGGAGAAAACGACAGCTATCACCACTGCCTGCAACATCCTGGCTAACCAGAGGTCCTGAGTCTGTCCACGTGAYAASTTCACTRCTAKYATAACCAGCATTCGAGAAARCCAGCACACTAAGTCTATCTACAACCAAGGAATCTCACAGAGTCTAYTTCACTCCCCTGCCACCTCCATCAGAGCTGGTGCTGGTATTCACCGCTGRGAGACTTGAAGACAGGTCACATCACWGGATYCYTTGCAGACATTCCCCAGYACCAGCCCGGAGTCTGGTAGCTCCACTGGGTGGCTAGAYCCAGAAGAGCAATAACGATCACTGYAGTCCGGCTCTCAGGAAGTCCCATTCCTAGGRGAAGGGGGAGAGCACCACATCAAGGGAACACCCTGTGGGACAAAAGAATCTGAACGGCAGSCCTTGAGTCCCAGATCTTYCCTCTGACATAGCCTACCCAAATGAGAAGGAACCAGAAAAAYAATTCTGGTAATATGACAAAACAAGGTTCTTTAACACCCCCAAAARATCACACTAGCTCACCAGCAATGGATCCAAACCAAGAAGAAATCYCTGAATTGCCAGAAAAAGAATTCAGAAGGTCGATTATTAAGCTACTCAAGGAGGCACCGAGGAAGGTGAANACCAACTTAAAGAAANTTANAATATAATACAGGATATGAATGRARAAATCTYCAGWGAAANAGATAGCATAAAKAAAAAACAATCAMAACTTCWGGAAATCGAAGYACACTTATAGAAATGCGAAATGCWCTGGAAAGTCTCARCAATAGAATNGAACAAGCAGAAGAAAGAACTTCAGAGCTCGAAGACAAGGYTTTCAAATTAACCCAATCCGACAAAGACAAAGAAAAAAGAATAAGAAAATATGAACAAAGCCTCCAAGAAGTCTGGGATTATGTTAAACGACCAAACCTAAGAATAATCGGCGTTCCTGAGNAAGAAGAGAAATCTAAAAGTTTGGAAAACTTATTCGNGGAAATAATCGAGGAAAACTTCCCCGRCSTTGCTARAGAYCTAGAYATCCAAMTACAAAGAAGCTCWAWRAACA >MER28 Nonautonomous DNA transposon CAGTTGACCCTTGAACAACATGGGTTTGAACTGCGTGGRTCCACTTATACGCGGATTTTCTTCCATCTCTGCCACCCTGAGACAGCAAGACCAACCCCTCCTCCTCCTCAGCCTACTCAACNTGAAGACGANGAGGATGAAGACCTTTATGATGATCCACTTCCACTTAATGAATAGTAAATATATTTTCTCTTCCTTATGATTTTCTTAATAACATTTTCTTTTCTCTAGCTTACTTTATTGTAAGAATACGGTATATAATACATATAACATACAAAATATGTGTTAATCGACTGTTTATGTTATCGGTAAGGCTTCCAGTCAACAGTAGGCTATTAGTAGTTAAGTTTTKGGGGAGTCAAAAGTTATACGTGGATTTTTNACTGCGCGGGGGGTCAGCGCCCCTAACCCCCGCGTTGTTCAAGGGTCAACTG >MER30 Nonautonomous DNA transposon CAGGGGTGTCCAATCTTTTGGCTTCCCTGGGCCACACTGGAAGAAGAAGAATTGTCTTGGGCCACACATAAAATACACTAACACTAACGATAGCTGATGAGCTWAAAAAAAAAAAAMAATCCCAMAAAAATCTCATAATGTTTTAAGAAAGTTTACGAATTTGTGTTGGGCCGCATTCAAAGCCATCCTGGGCCGCGTGCGGCCCGCGGGCCGCGGGTTGGACAAGCTTG >MER31 a consensus CACCCTGCGTATCTGAYCAGGTTCCTCARTCTCCGCCGTCCCCCAGGTAATGTTTGRTCACCTGGCCTGCCTTCAGCTAGAATCCTRTTAGGTCAGTTTAGCAGAATCTCCTTACTCCTGATGTTTCCTCTTRGTAATTTTCCATCCACTGCCCCCGYACTCTGCTCYTTGGCTATAAATCCCCACTTGTCCTTGCTRTATTCGGAGTTGAGYCCAATCTCTCTCCCCTACCGYAAGACYCCATTGCAGTGGTCCCTGTAYCTRTCGNRATGGTCCTGAATAAAGTCTKCCTTACCATGCTTTAACAAGTRTYATTGAAWAATTTTTTTT >MER33 Nonautonomous DNA transposon CTGCGCTGTCCAATACGGTAGCCACTAGCCACATGTGGCTATTGAGCACTTGAAATGTGGCTAGTCCAAATTGAGATRTGCTGTAAGTRTAAAATATACACCAGATTTCAAAGACTTAGTACGAAAAAAAGAATGTAAAATATCTCATTAATAATTTTTATATTGATTACATGTTRAAATGATAATATTTTGGATATATTRGGTTAAATAAAATATATTATTAAAATTAATTTCACCTGTTTCTTTTTACTTTTATTAATGTGGCTACTAGAAAATTTAAAATTACATATGTGGCTCGCATTRTATTTCTATTGGACAACGCTG >MER34 a consensus TAGGGGAGTGAGAGATCACCTCCAACCCTCCCTCCCCTTTGCTCTTATGTGGGCTCTTTGGTGAATCTAGGAACCAAATTGACACAAGGCAGATTAACAAGAGAAAAGCATACAAATTTCATTAGTTTTACATGTGCATAGGAAYCCTCACAAAAGTCAAGYCCAGAGAAGCGGCCAAAGTAAGATGCYTTTATACTTTTTNNACAAAGNACAATAARYTNNRRAARTGTCAGAAGAAAGGGGATCTGGCTAGGGGCAGTAAATTTCTCGCGGAGTCATTAGGAGAAAAGTGTGTGTGTTGGGGTGTAAACTTAGTGGAAGATAACGGTTACTTCGGAAAGTATATTCAGGTCCAAAGCAGCCCCCAGTTTCGTCTCTGGTGATAGGTGCTATTTTCTCCCTCTGGTAAGGGACGGGTACTCCAGAGGAATCCTGGAGGAAGCTTTATGCGCTTGCTGCATGCAGGAAGAGATAGGTCATCAAACCTTTTCTGAAACTACAATTTCTCCAATATTTTCAACTCGAAATAATCAATGTACCAATTCCATATATTTTTGGGATGGCACATCCATCAGTCCTTC >MER35 a consensus GATGTGGCGTTTGAGTGGACACGGGGGAAGAAACAAGTAATATGAATAACATGGTGAGACAGAAAGAGTTGTGGACAGAGCTGTGGGAAATATGAGAGATAAGGAGAGAGATACTGAAAAGAGCGTTAAGAGAGATGAAGATAGGGTGTCTGGCCCATGAAGGCCCTCGTGAAGAGCAATGCTGAATAGATGAATGAACCTCAATGCCCAGCAGTGGTGGGATGAAAAGGGGATCCTGTGCAGAAACCACACTACCCATCAGAGAAGCAACTCTGCTCGTTTCCCCTTGA >MER39 a consensus GGGCATTANNNCTGTAGGGGCAGAAAGGTGTGATNCCTTCCCTACCCATCATAAGGGTCACGGCNNACACTCCTATAATAAAANAYAGGTTAGCAAGAGAAAAGCATAAYAGATTTATTTAATCAAAGTTTTATGTGACATGRGAGCCTTCAGAAATGAAGACMCAAAKACTCAGGGAAAACTCTATTTTTATGYTTAGATTTGATGAAGAATGGANAGCTGCGCAGAAATGTGATTRGACAAAAGGGGWATGATCTAATGGTMATARACTGRGTGTGGRAACCCAGCAAGGCTTGTCTGTTCGTATTCTTCTTGGTCTMTCTGTGCGGCATTCCTTCCTCCCGGGTATAGGGCGGGACCCCTTCTGGAATGAGGGTCTTATGACCTACWATCAGACGAGATAGGTCAGAWAATTTCTTTACGGSCAGCYCYAAGACAGAAAGGCGGGKRAWGATTAGAGGGCNAGAGAAGGTCAGAAAATGANNTTCTGTTTYCTGAGGNCTGCTTCTGAGGCTTAGAGCGCCCCAACATTNTAACAAAAGNCTGTCCNTCACCTTTACCGCTCTGAAGCTGTTCCAAAGCTGCTACATGAACCAAGGACAAAAAGCCANATANTTCAACAAAAGATATNCTTATNGTTTTAGTCACTCAGGAAACAGCAAGGCTTATAGGAGCTGAGAACCCGGAATTATG >MER41 Medium reiteration sequence MER41 AACCTGCCAAGAACAGAAACTGGCAGAATCTGTCACAAGAAAAAGTCCCAATCAGACTTCAAGAGAGGGTTCTTGGATCTTGTGCAAGAAAGAATTCATGGGGAGTCTGAGTAAAGTGAAAGCAAGTTTATTAAGAAAGAGGAATAAGAGAATGGCTACTCCAAAGACAGAGCAGCCCTGAGGGCTGCTGGTTGCCTATTTTTATGGTTATTTCTTGATTATATGCTAAGCAAGGGGTGCATTATTTAAGCCTCCCCTTTTTAGGCCATATGGGGTTATATCCTGATGTTACTATGGCATTTGTAAACTATTATGGTGCTGGTGGGAGTGTAGCAGTGAGGACGACCAGCAGTCACTCCCATGGCCATATTGGTTTTGGTGGGTTTTAGCCGGCTTCTTTCTGCAACCTGTTTTATCAGCAAGGTTTTAAGGACCTGTATCTTGTGCTGACCTCTTATCTCATCCTGTGACTTAGAATGCCTTAACTGTCTGAGGATGCAGCCCAGTAGGTTTCAGCCTTATTTTACTCAGCTCCTATTCAGGATGAAGTTGCTCTAGTTCATATGTCTTAGACATTTCCTCCTTCCCTTT >MER42a MER42 repetitive sequence: subfamily MER42a - a consensus ATGTAYAGKAGGATTGATCAAATAAGTAAACGTATTNAAGATAATGGGAGCCAGGTTTCTCACTGTCGGAGAAGGGAGTTACAAATATGGAAAGGGRGAAGRCTAGAATGAACCCTGTGGTATTRGATTRGAATTGGAGGTATCAGTRTGAACTCATGRTTTTTAATATAYRYRYRYRYRTTTTTCCTAGTCCTGTCCACTGAGAGGGCCTAGAAGCAACGACACCCCAGTAGCAACGAGCACACCTAGCACCCAGATCTTGGTTTCTAATACCATTCTCCAATAAAAGGAACCAGGGCTCCTTGGAGAAATGGCTGATTCTAGGACTAGGGCAGGAAATATACAAGATGAGCCTGGAACATCTTGTARTGCCAGAAAATAAGGAAGTGCTCAAAAAACGATRRGGGTATGTCAAARGGACACAGAAGCCAACTGAAAGAGCTCCCAATGGCCAAAGCTGGAACAATTTGAGCAACAAAATAAATTATRGTAGTATTGGATTATAACTCAAAGTATAAAATAAATATCCATGAGTTCATACTAATACAAATAAATGATTAAATAAATAAATAAATTGAGAARATAAATAAGTCTTTCTTGCAGAAGAATTTCAAATAATTTATGTAGATARCCTCCCCTCAAGGAAGTGGAGCGTAAYTCCCCACTCCTTAAGTGTGGGATGGRTATAGTGACTTCCTTCYAGAAAGNATAGTNTAGGACGGAARAAAAAGTAAATTTACAGTAGAGAAACCTGACAAACACTACCTTAGCCARRTAATCAARGTTAGCATCAACGATAATARGTCATGYTGATARTATATANCCTTRATATGATGTGATAAGAATGGCACTTTACCTCCGTGGTCTTCCTCCCAAAAACCCATAACCCCAGTCTAATCATRAGAAAATATCAGACAAATTCCAACTGAGGRACATTCTACAAAATACCTGACCAGTACTCCTCAAAACTGTCAAGGTCATCAAAAACAAGRAAAGTCTGAGAAACTGTCACAGCYAARAGGAGCCTAARGAGACATGACAACTAAATGTAATGTGGTATCCTGRATGGGATCCTGGAACAGAAAAAGGACATTAGGTAAAAACTAAGGAAATMTGAATAAAGTATGTACTTTAGTTAATAATAATGTATCAATATTGGTTTATTAGTTGTGACAAATGTACYATANTAATGTAAGATGTTAAYARTAGGGGAAACTGGATGTGGGGTAYATGGGAACTCTCTGTACTATTTTTGCAACTTTCCTGTAAATCTAAAACTATTTTAAAATAAAAT >MER42c MER42 repetitive sequence: subfamily MER42c - a consensus ATGTAYAGKAGGATTGATCAAATAAGTAAACGTATTNAAGATAATGGGAGCCAGGTTTCTCACTGTCGGAGAAGGGAGTTACAAATATGGAAAGGGRGAAGRCTAGAATGAACCCTGTGGTATTRGATTRGAATTGGAGGTATCAGTRTGAACTCATGRTTTTTAATATAYRYRYRYRYRTTTTTCCTAGTCCTGTCCACTGAGAGGGCCTAGAAGCAACGACACCCCAGTAGCAACGAGCACACCTAGCACCCAGATCTTGGTTTCTAAATACCATTCTCCACTAAAAGGAACCAGGGCTCCTTGGAGAAATGGCTGATTCTAGGACTAGGGCAGGAAATGTACAAGATGAGCCTGGAACATCTTGTTRTGCCAGAAAATAAGGAAGTGCTCAAAAAACGATRRGGGCATRTCAAAAGGACACAGAAGCCAGCTTGAAGGGGCTCCCACTGGCCAAATCTGGGACAATTTGAGCATCAAAATAAATAATRGTAGTAATGGATTATAACTCATTGAATAAAATAAATATCCATGAGTTCATACTAATATAAATAAATGAATAAAAWAAATAAATGAGAARGGAAAGCTCTTCTTACAGTNGAATGCCAANTAATAAATNTAGAARGAATAATAATAGAAAAATCACCATTAGGCAAAYACCGCAGTAATAACTGTTTYAGGCAAGATCCATCGATAGATGCTMTAATTAGTGGGCGAAARTTTGATGAGAAACGGRATATTTGCATAGTCTCAAAGTATCTCCTCACAAGATATTTATTAATTACAAAGGGAAAAAYAGTGACTTTACAGTAGAGAAACCTGGCAGACACCACCTTAACCAAGTGATCAARGTTANCATCACCAAAAATGAGACAAAYTGACATCATGCGCYNCCTGATRTGATGCGCCGAGAAGAACAACATSGCTTCTGTGATATTCCTGCCAAAGATGCATAACCTGAATCTAATCATRAGAAAATATCAGACAAACCCAAATTGAGGGACAKTCTACAAAATAACTGGNCTGTACTCATCAAAARTGTCAAGGTCATAAAAGACAARGAAAGACTGAGGAACTTTCTACNTTTGACGGAAGACTAGARNCATGACAACTAAATGCAACGCGGGATTCTGRANTGGATCCTGGRTCGAGAAATAGTGGGAGTTTKTAYCTATAAAGGACATTATTGGGACANTTGRCGAAATTTGAATANGGTCTGNAGATTAGATAATAGTATTGTATCAATGTTAATTTCCTGATTTTGATAATTGTAYTGTGGTTATGTAAGAGAATGTCCTTGTTTTTAGGAAANACACACTGAAGTATTTAGGGGTAAAAGGNCATSATGTCTGCAACTTACTCTCAAATRGTTCAGRAAAAAAAATNNATATAYATAARYAGAGAATGATAAAGCAAATGCGGYAAAATGTTAACAATTGGTGAATCTGGGTGAAGGGTATACGGGWGTTCTTTGTACTATTCTTGCAACTTTTCTGTAAGTTTGAAATTACTTCAAAATAAA >MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus CCCAAGCCCAGTAACGCTGTGGCTCTTGCAGACTCNTAGAGGTACTGCCTTNRTGGTCTTGGATAAGATCTGGAAGAATTCCCTGGATTACCAGGCAGAGACTCTTGTTCTCTTCCCTTACTTTCTCCCAAACAAACAAATGGAGTCTCTCTCTCTNTCTGTGCTGAGCTGCCTAGANCTGNGGGWGGGGTGACACAAGCACCCCTGTGGCCACCACCACTGGGACTGTGCTGGGTCAGACCTGAAGCCAGCACAGCATTGGGTCTCACCCA >MER44A Nonautonomous DNA transposon CAGTAGTCCCCCCTTATCCGCGGGGGATACGTTCCAAGACCCCCAGTGGATGCCTGAAACCACAGATAGTACCGAACCCTATATATACTATGTTTTTTCCTATATATATATACCTATGATAAAGTTTAATTTATAAATTAGGCACAGTAAGAGATTAACAATAACTAATAAAATAGAACAATTATAACAATATACCGTAATAAAAGTTATGTGAATGTGGTCTCTTTCTCTCAAAATATCTTATTGTACTGTACTCACCTATTTTCGGACCGCGGTTGACCATGGGTAACTGAAACTRCGGAAAGTGAAACCGCGGATAAGGRGGGACTACTG >MER44C Nonautonomous DNA transposon CAGTAGTCCCCCCTTATCCACGGGGGATACGTTCCAAGACCCCCAGTGGATGCCTGAAACCACGGATAGTACCGAACCCTATTGCTATCAGTCTTTTGGAACATGTTTCTGTTCATGTCTTCTGCCCACAAATTTAATGCCTCTTCCACCTCAACTAAACACGTATCATGCACTCCGGCCATCACTTTTGCAGTTTGAGGTATGACATCAAAACTAGCATGAATTTATTTTTCCTTCTTCACAATTCCAAAGATGGAAGATTCTTTCTTACCGTAGATCTTAGCAACCTCAGCATACGATTATTTTTCYTTTCTTTTTAAGTCAAGAACTTTCACCTTTTCGCTTAAAGGAAGCACTTTACAGCTTCTYTTTGGCATGTCCGAATTGCCACCAACACTACTCTTGTGCTTTTGGGCCATTATTAAGTAAAACGGGAGTTACTTGAACACAAGCACTGTGATACCAAGACAGTCGGTCTGATAACTGAGATGGCTACTAAGTGATTAACAGGCAGGTAGCGTANACGGCGTGGATACGCTGGACAAAGGGATGATTCACATCCCGGATGGGACGGCGCGAAATTTCATCACGCTACTCAGAACGGCGCGYAATTTAAAACTTATGAATTGTTTATTTCTGNAATTTTCAATTTAATATTTTCGGACCGCAGTTGACCACGGGTAACTGAAACCACAGAAAGTRAAACCACGGATAAGGGGGGACTGCTG >MER45 Nonautonomous DNA transposon CAGGACCGGCTACATAATTTGCGGGRCCCAGTGCAAAATGAAAATGCGGGGCCCCTTGTTCAAAAATTATTAAGAATTTCAAGACGGCGACAGCAGAGCATTAAACCAAGCACGGGGCCCTTCTRAGCGCGGGGCCCTGTGTGACTGCACAGGTCGCACGCCCATGAAGCCGGCCCTG >MER46 Nonautonomous DNA transposon CAGGTTGAGTATCCCTTATCCAAAATGCTTGGGACCAGAAGTGTTTCGGATTTCGGATTTTTGGATTTTGGAATATTTGCATTATACTTACCGGTTGAGCATCCCAAATCTGAAAATCCGAAATCCAAAACGCTCCAATGAGCATTTCCTTTGAGCGTCATGTCGGCGYTCAAAAAGTTTCGGATTTTGGAGCATTTCGGATTTCGGATTTTCGGATTWGGGATGCTCAAYCTG >LOR1 Human low copy repetitive sequence - a consensus TGTTACTGAACACCAGGGGTTCGGTCTAGGTCCTGCTGCTCGCCACACAGAAAGCCAATCACTGAGACAATAAGTATTGCCAAGGAAGAAGGCTTTAATCGGGTGCTGCAGCCGAGGAGACGGGAGATCAGTCTCAAATCCATCTCCCTGACCGACTAAAATTAGGGGTTTATATAGCAGGGAAGAAATGTAACCGTGTGTGGGAAAACAGGAATTAGGGAGGGGTAAGGAAGAGAATTTGGTCAACAGGAAGCAGGTGGTCAGTTAGGCAATCATGATGGGTGAGGGGTCTGACGTCTCATTGTCCGGATGCAGTGATCTGGTAAGTTTCAGCTCCTTGATACTATCTGGGAGGCCTGATGGTTGGTTTCCTGAAAAAGGAACTCAGATAAGACAAATGTAACTTTCTCGAGTTTTAAGACTGGGAGAATCARTTTCTATGTTTATTCAAAGAAACCATAAACATCAGTTCTATGGGACAATTGGGTCAGTTTCAATTG >SVA Composite retroposon CTCCCTCTCCCTCACCCTCTCCCCATGGTCTCCCTCTCCCTCTCTTTCCACGGTCTCCCTCTGATGCCGAGCCGAAGCTGGACGGTACTGCTGCCATCTCGGCTCACTGCAACCTCCCTGCCTGATTCTCCTGCCTCAGCTTGCCGAGTGCCTGCGATTGCAGGCGCGCGCCGCCACGCCTGACTGGTTTTCGTATTTTGTTAGTGGAGACGGGGTTTCGCTGTGTTGGCCGGGCTGGTCTCCAGCTCCTAACCGCGAGTGATCCACCAGCCTCGGCCTCCCGAGGTGCTGGGATTGCAGACGGAGTCTCGTTCACTCAGTGCTCAATGATGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCGCTACAACCTCCACCTCCCAGCAGCCTGCCTTGGCCTCCCAAAGTGCCGAGATTGCAGCCTCTGCCCGGCCGCCACCCCGTCTGGGAAGTGAGGAGTGTCTCCGCCTGGCCACCCATCGTCTGGGATGTGAGGAGCGTCTCTGCCCTGCCGCCCATCGTCTGAGATGTGGGGAGCACCTCTGCCCGGCCGCCCCGTCCGGGATGTGAGGAGCGTCGCTGCCCGGCCGCCCCGTCTGAGAAGTGAGGAGACCCTCTGCCTGGCAACCGCTCCATCTGAGAAGTGAGGAGCCCCTCCGCCCGGCAGCCGCCCTGTCTGAGAAGTGAGGAGCCCCTCCGCCCAGCAGCCACCTGGTCCGGGAGGGAGGTGGGGGGGTCAGCCCCCCGCCCGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGGGTCAGCCCCCAGCCCGGCCAGCCGCCCCGTCCGGGAAGTGAGGGGCGCCTCTGCCCGGCCGCCCCTACTGGGAAGTGAGGAGCCACTTTGCCCGGCCAGCCACTCTGTCCGGGAGGGAGGTGGGGGGGTCAGCCCCCCGCCCGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGGATCAGCCCCCCGCCCAGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGGGTCAGCCCCCCGCCCGGCCAGCCGCCCTGTCCGGGAGGTGAGGGGCGCCTCTGCCCGGCCGCGCCTACTGGAAAGTGAGGAGCCCCTCTGCCCGGCCACCACCCCGTCTGGGAGGTGTGCCCAACAGCTCATTGAGAAGGGGCCATGATGACAATGGCGGTTTTGTGGAATAGAAAGGGGGGAAAGGTGGGGAAAAGATTGAGAAATCGGATGGTTGCCGTGTCTGTGTAGAAAGAGGTAGACCTGGGAGACTTTTCATTTTGTTCTGTACTAAGAAAAATTCTTCTGCCTTGGGATCCTGTTGATCGGTGACCTTACCCCCAACCCTGTGCTCTCTGAAACATGTGCTGTATCCACTCAGGGTTGAATGGATTAAGAGCGGTGCAAGATGTGCTTTGTTAAACAGATGCTTGAAGGCAGCATGCTCCTTAAGAGTCATCACCACTCCCTAATCTCAAGTACCCAGGGACACAAACACTGCGGAAGGCCGCAGGGTCCTCTGCCTAGGAAAACCAGAGACCTTTGTTCACTTGTTTATCTGCTGACCTTCCCTCCACTATTGTCCTGTGACCCTGCCAAATCCCCCTCTGTGAGAAACACCCAAGAATGATCAATAAAAAAAAAAAAA >ALR Human alpha repetitive DNA - a consensus AATTCTCAGTAACTTCCTTGTGTTGTGTGTATTCAACTCACAGAGTTGAACGATCCTTTACACAGAGCAGACTTGAAACACTCTTTTTGTGGAATTTGCAAGTGGAGATTTCAGCCGCTTTGAGGTCAATGGTAGAATAGGAAATATCTTCCTATAGAAACTAGACAGAAT >MSR1 Human 37 BP minisatellite repeats, specific to chromosome 19 AGTCAAGACCCCCAGCCCCTCCTCCCTCAGACCCAGGAGTCAAGAACCCCCAGCCCCTCCTCCCTCAGACCCAGGAGTCAAGAACCCCCAGCCCCTCCTCCCTCAGACCCAGGAGTCAAGACCCCCCAGCCCCTCCTCCCTCAGACTCATGAGTCCAGACCCCCAGCCCCTCCTCCCTCAGACCCAGGAGTCCAGACCCCCAGCCCCTCCTCCCTCAGACCCAGGAGTCCAGACCCCCAGCCCCTCCTCCCTCAGACCCAGGAGTCCAGGCCCCACCCCTC >TAR1 Human telomere associated repeat sequence, complete sequence GATCAACAGTGAGGAGGTCCCACAAGGCTAAGTGGGGCAAGTCGGGGACCTAAGGCAGTAGCAGGAAAACCAAAGAAAACAGGCGGAGACTTGAGACAGAGGCAGGAATGTGAAGAAGTCCAAAATAAAAATCCCTGCACAGGACTCTTAGGCTGTTATCATGCACTATCAGCCTACTCCTCCCTATTTTTGTACAATAAGCTCTTTACACTGTATTTCTTTTCAATGAAGTTATCTTCCATCTTTGTACTGCCTCTTGGTGAAAAGCTGTCTTCCAAGTTAATAACTGGGACATCAGCTCTCTGCAGTAATAGCTCCTTTTCAGTTTTAATTTGCAGAACTGATGGGGATTAATAACTGGCGCTCTGACTTTAAGTGGTGCAGGAGGCGGCCAGTAGGGGACGCCAGCCGTTACGCCGGGAGCAAGAGGGCCCTGCGTAGTCCCCATCTGCCTGCATGTGGCGTGCAGCCACGACAATGGCAGCAAGAGGGCCCGGCAGTGTGCCCAGCTGCCAGCAGGCGGGTGTGCTGCCACTATAATGTGAGGAAGAGGGCCCTGCAATGTCCCTAGCTGCCAGCAGGCGGCGTGCCACCACTATACTGCGAGCAAGAGAGCCCTGCCGTGCCCCGGCGCCAGCAGGGGGCGCTGGACAGCACTGTAAGCAAGAGGGCCCTGCAGTTGTCCTAGTCGCCAGTAGGGGACGCAATGGCAGAGCACCGTGGGCAAGCTGGTCCTGTAGTGCCCGGCTGCAAGCAGGGGGCGCCCGAAACGGGCTTTTCAGATTACTCAGGTTCCACTCGTCTCTGCGCCGCCGGGGACGTGTGTCTCTGCGCGTGCACCGCGCCACCCCCGCGCTCCCCGCCCGGCGGCGCGCGACTGTGCGACTGCAACACTCCCCGCCACCCTCAGCCCAGCGACGTGCGTCTCTGCGCCTGCGCCGCGCCTCACTCCCGCCCGCTCAGCGACCCCTCCCTTCCGGGGAGGCGCCGGCGTGCGTCTACGCCCTGCGCCGCGTCTCCCCAACAGCGGCGCGCCTCTCTGCGCCTGCGCCGGCGCGCCGCGCCTCTCTGCGCCTGCGCCGGCGCGCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCCCCGCGCCTCTCTGCGCCTGCGCCGGCGCGCCGCCTTTGCGAGGGCGGAGTTGCGTTCTCTTTAGCACACACCCGGAGAGCATCGCGAGGGCGGAGCTGCGTTCTCCTCTGCACAGACTTCGGGGGTATTGCGAAGGCGGAGCAGAGTTCTTCTCAGGTCAGACCCGGGCGGGCGGGCTGAGGGCACTGCGAGGGTGGAGCTGCGTTCTGTTCAGCACAGACGTGGGGGGCACCGTAAAGGCGGAGCAGCATTCTTCTCAGCACAGACGTTGGGGGTACTGCCTGCCTTTGGGATAACTCGGGGCCGCATCGAGGGTGAATAAAATCTTTCCCGTTTGCTGCCCTGAATAATCAAGGTCAGAGACCAGTTAGAACGGTTTAGTGTGGAAAGCGGGAAACGAAAAGCCTCTCTGAATCCTGCGCACCGAGATTCTCCCAAGGCAAGGCGAGGGGCTGTATTGCAGGGTTCAAGTGCAGCGTCAGAACTCAAATGCAGCATTCCTAATGCACACATGACACCCTAAATATAACAGGCATATTACTCATGGAGGGTTAGGGTTCAGGTTCGGGTTCGGGTTCGGGTTCGGGTT >CER Human D22Z3 repetitive DNA (centromeric DNA) CTGCAGAACACTACTTCCAGGGTCTAAATGTCTGTCCTTCACATAGGATTCCAGAACACTGCTACGAGAGTGTGAATGTTTGTCCTTCACATAGCATTTCACAACTGCCATGGTCTGAATGGTTGTCCCTCACATAGTATTCCAGAACACTGCTATGAGGGTCTGAATGTTTGTACCTCACATAGGATTCCAGAACACTGCTATGAGGGTCTGAATGTTTGTACCTCACATAGGATTCCAGAACACTGCTATGAGTGTTTGAAAGTTTGTCCCTCACATAGGATTCCAGAAGACTGCTGCTGGGGTCTGAATGTCTGTCCCTCACATCGGATTCCAGAACCCTGCTGCTGGGGTTTGAATGTCTGTCCCTCACATAGAATTC >BSR Human beta satellite DNA - a consensus GATCAGTGCAGAGATATGTCACAATGCCCCTGTAGGCAGAGCCTAGACAAGAGTTACATCACCTGGGT >HSATII Human satellite II DNA ANTCCATTCGGGTCCATTCGATGATGATCACACTGGATTTCATTCCATAATTCTATTCG >HSATI Human satellite I DNA HinfI fragment GANTCNGCTCNNGACACAACTCTCTGAGCCTTCGGTGACCTTTCNGTCTNGTGTAANNGCACTCNTGTCYYCCTGGCAGCAGTTGGACCTCGCGATGTGGATTGTGCCCTCACCCAGCNNTNTTTATGCCCTNTCNCCATGGTGACNGGATTAGGGGTCTCCTGCTCTTCNNTCCTAAGGACCACTGTCTGTGCTGTGTCTTTCAAAGGTCAGAAGAGATTGNACCTTTGTGTTTTTATTTTCCCTGTGTTTGCTTTTTCTCNNTGGGGAACCTGTGTTGCTGCTTTGAAGGTATATTCATACTGGCCNNTCANNTGNNCAACATCNNCAAANTNACTAGTTANGGCTTTCAAAATATGNTATTTCAAAAAATTANCCGTCTGTATTTTCCATATGCAGTTATAAGTAGGTTTCATCGGTTATGTTTTATTCCTCAGTTTATACATTTGATTATTGTACCAAGCAGAGTACCTTTGAAATTTTTTTTCATTTAAAAAATATGGATCTTTAAAAAAAAATTATATATGATATATATTACATGTTATGTGCTATGCCTTATNTGTAACATATAACCTCATATATTTTNTATGTCATAGTATATAATATTTATCCTGTGTCATCTTATATATAC >R66 A 66bp tandem repeat - a consensus ACAGAGTGCTGATTGGTGCRTTTACAAACCTTTAGCTAGACACAGAGCGCTGATTGGTGCGTTTTT >SAR Human satellite I DNA ACATAAAATATCAAAGTACATCAAATATATATTTTATTCTGT >SN5 Human centromeric SN5 satellite DNA sequence CTTCCCTTCAGGGACCTCAAAGTGACCAGCTTCCCCTTGAAGAATGACTCTCCAAGGCCCAGGAGCCCAGCTTCTGGGCCTCCAAGCCAGGCCATCTGGCGAGGGAGTCGGTGGACGTGCCCTGGCTTCTTCCATGTTGAGTTGGTACTACCCACCAAGGGGGGTAGAGAGGCGAGCAGATGTTGTCTCTGGCCTGTGTCTTGTTATCATGGTGCTGACTAGGCCTGGTACAGGGCCCTGATGGGGTTGTCCTGGGTGGTCACGGGGGTGATGAGAAGAAGATGCAGAATGGATTGCTGTGAGGATGAATGAGACGACTGTCAGTACAGACAGGCACACGGTGAAGTGTTCAGGGATTCCCCTCAGTAGCTGCCCAGACCCAAAACCTGACTCCTGAGTCACGTTACTGTCCCACTATACGTTAAGAGGAGGGAAAGCTGGGTCGCGCAGGTCCC >HY3 Ro(hY3) pseudogene - a consensus TAGTCAANTGAAGCAGCGGGAGTGGAGAAGGAACAAAGAAATTTGTAACTGGTTGTGATCAATTAGTTGTAAACANCGCTGCACTCCAACCAGCC >HIR Human HINF family repeat DNA GATTCCCAGGTGCACAGAGATCCTAATCCGCATCCATCGAAATCTCACAAAGTGTCCATAAATCACTCAGGGAGGGCCCCCATGGATACAGGGCCGTAGTAGGATGCTCCTATAGTGGGCATTAATATGAGAATGACCGAAAAGTGCATTTAGGACCATATTATAATTTTCGGGTTCCCAGGTGCACGTTTCCAATAACCAGGTGCACGGATGTATAGGGTCCCCCCCATGGATAGAGGTCCGTGTTAGGGTGCTCCATATCGGGCATGAATATCAGGAACACCGGCATGTGCACTTAGGACCATGTTTTAATTTTTCA > satellites 2 and 3 GGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAAT >IVR Repetitive sequence from human involucrin gene - a consensus CAGGAGGGGCAGCTGAAGCACCTGGAGCAG >7SK Human family A3 repeated element DNA; 7SK RNA pseudogene GATGTGAGGGCAATCTGGCTGCGACATCTGTCCCCACTGATTACCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTCTCCTCTTCCTCCCTCAGCCCTCCATGTGTGTACCTCCTGAAGCGGACTGGTCTTCAGTCAAGGGTAGACGAGTAGCTGTGCTCCCCTGCTAGAACCTCCAAACAAGCTCTC >KER Human K element interspersed repeat DNA AGCTCATCTGTCCACTGAAGATGCTTGGACAGAGTTAGGAATGCTTCCTGGGAGAGGTAACATTTGAGACTTTCCTGGAAGAATGGTCAGAGTAAACCAAGTAAGTAGGAATGGAAAGAGGATGGGAGGCCCCAGCTTCCCAGAGGCATAAGGTGAGGANGNCCCTATGCATTCAGATGTGGCCCACCCTGGGGTCTGGTGGACTAAAGNCTTGGACACCCCAGATCAGCCTTAGTGGGATGAGGCAGGAAAGACAGCTGAGGGTCAGAACCCAGGCAGGTCCAATGCCAGGGTGGGCATTTCGAGTTGGTGAGACATTTCACCCTGGTGCCAAGCT >XTR Repetitive element X-2 from human alpha-fetoprotein gene TTAATTTGAGAGAAATTAATTATTCTGCAACTTAGGGACAAGTCATGTCTTTGAATATTCTGTAGTTTGAGGAGAATATTTGTTATATTTGCAAAATAAAA >XBR Xba repetitive element copy A, from human alpha-fetoprotein gene AATTGATAGGTCACGTTCTCACTCTATTTGCCTTTAAGGGAAGAAAGCAATCAAGTTAATATGTTTTCCTTCATTGTATAGTATGTAACTACGGACACTATTAGAGGAGGGATTTGTGTAGCACTTAGGACATTATACTTGATAATTTCCAAGGGTCTTTCTAGATTTAAAAGTCTGATTCTAACGTAGTAATAAAAATAAAGGCCCAATTTTCTCTTTAATATTGCCTGAAGATATTACTCTATTATTGCATTAAAATTAAACATTCACACATTGTTTGCACTGCTAAATAAAATTATGTAA >MARINER1 Human mariner TTAGGTCGGTRCAAAAGTAATTRCGGTTTTTGCAYTGTTGGAATTTGYCATTTGATATTGGAATACATTCTTAAATAAATGTGGTTATGTTATACATCATTTTAATGGGCATTTCTCGCTTTACGTTTTTTTGCTAATGACTTATTACTTGCTGTTTATTTKRTGTTTATTTTAGACTATGNAAATGATGTTAGACAAAAAGCARATTCGAGCGATTTTCTTATTCAAGKTCAAAMTGRRTCGTAAAGCGGCGGAGACAACTCGCAACATCAGCAACGCATTTGGCCCAGGAACTGCTAATAAACATACAGTGCAGTGGTGGTTCAAGAAGTTTTNCAAAGGAGACGAGAGCCTTGAAGATGAGGAGTGTAATGACGGGCCATCAGAAGTTGACAACGACCAGTTGAGAGCAATCATCGAARYCGATCCTCTTACAACTANACGAGAAGTTGCTGAAGAACTCGACGTTGACCATTCTACGGTCGTTCGGCATTTGAAGCAAATTGGRAAGNAAAAACTCGATAAGTGGGTGCCTCATAAAGTGAGCAAAACTTTTTNAAATTGTCATTTTAAAGTGTCATCTCTTATTCTATGCAATAACAACGAACCATTTCTTGATCAGATTGTGACGTAGAACGAAAAGTGGATTTTATACGACAACCGGCGACGACCAGCTCAGTGGTTGGACCGAAAGAAGCTCCAAAGCACTTCCCAAAGCCAAACTTGCACCAAAAAAGTCGCGGTCGCGGTCTGGTGGTCTGCTGCCAGTGTGATCCACCACAGCTTTCTGAATCCTGGCAAAACCATTACATCTGAGAAGTATCTTCAGCAAATTGATGAGATGCACCAAAAACTGCAATGCCTGCACCTGGCATTGGTCAACAGAAAGGGCCCAATTCTTCTCCACGACAACGCCCGACCGCACGTYGCACAACCAACGCTTCAAAAGTTGAATAAATTGGGCTACGAACTTTTGCCTCATCCGCCATATTCACCTGACCTCTCGCCAACAAACTACCACTTCTTCAAGCATCTCGTCAACTTTTTGCAGGGAAAACGCTTCCACAACCAGCAGGATGCAGAAAATGCTTTCCAAGAGTTCGTCGAATCCCAAAGCACGGATTTTTATGCTACAGGAATAAACAAACTTATTTCTCGTTGGCAAAAATGTGTTGATTGTAATGGTTCCTATTTTGATTAATAAAGATGTGYTTGAGCCTAGTTATGATTATGATTTAATGGCCAAAACCACAATTACTTTTGCACCAACCTAA >Made1 Human mariner derived element 1 - a consensus TTAGGTTGGTGCAAAAGTAATTGCGGTTTTTGCCATTACTTTYAATGGCAAAAACCGCAATTACTTTTGCACCAACCTAA >Tigger1 Autonomous DNA transposon CAGGCATACCTCGTTTTATTGCGCTTCGCTTTATTGCGCTTCGCAGATACTGCGTTTTTTACAAATTGAAGGTTTGTGGCAACCCTGCGTTGAGCAAGTCTGTCGGCGCCATTTTTCCAACAGCATGTGCTCACTTCGTGTCTCTGTGTCACATTTTGGTAATTCTCGCAATATTTCAAACTTTTTCATTATTATTATATCTGTTATGGTGATCTGTGATCAGTGATCTTTGATGTTACTATTGTAATTGTTTTGGGGTGCCACGAACCGCACCCATATAAGACGGCGAACTTAATCGATAAATGTTGTGTGTGTTCTGACTGCTCCACCGACCGGCCGTTCCCCCGTCTCTCTCCCTCTCYTCGGGCCTCCCTATTCCCTGAGACACAACAATATTGAAATTAGGCCAATTAATAACCCTACAATGGCCTCTAAGTGTTCAAGTGAAAGGAAGAGTMRCACATCTCTCACTTTAAATCAAAAGCTAGAAATGATTAAGCTTAGTGAGGAAGGCATGTCAAAAGCCGAGATAGGCCAAAAGCTAGGCCTCTTGCGCCAAACAGTTAGCCAAGTTGTGAATGCAAAGGAAAAGTTCTTGAAGGAAATTAAAAGTGCTACTCCAGTGAACACACGAATGATAAGAAAGYAAAACAGCCTTATTGCTGATATGGAGAAAGTTTTAGTGGTCTGGATAGAAGATCAAACCAGCCACAAYATTCCCTTAAGCCAAAGCCTAATCCAGAGCAAGGCCCTAACTCTCTTCAATTCTATGAAGGCTGAGAGAGGTGAGGAAGCTGCAGAAGAAAAGTTTGAAGCTAGCAGAGGTTGGTTCATGAGGTTTAAGGAAAGAAGCCATCTCCATAACATAAAAGTGCAAGGTGAAGCAGCAAGTGCTGATGTAGAAGCTGCAGCAAGTTATCCAGAAGATCTAGCTAAGATCAYTGATGAAGGTGGCTACACTAAACAACAGATTTTCARTGTAGATGAAACAGCCTTCTATTGGAAGAAGATGCCATCTAGGACTTTCATAGCTAGAGAGGAGAAGTCAATGCCTGGCTTCAAAGCTTCAAAGGACAGGCTGACTCTCTTGTTAGGGGCTAATGCAGCTGGTGACTTTAAGTTGAAGCCAATGCTCATTTACCATTCTGAAAATCCTAGAGCCCTTAAGAATTATGCTAAATCTACTCTGCCTGTGCTCTATAAATGGAACAACAAAGCCTGGATGACAGCACATCTGTTTACAGCATGGTTTACTGAATATTTTAAGCCCACTGTTGAGACCTACTGCTCAGAAAAAAAGATTCCTTTCAAAATATTACTGCTCATTGACAACGCACCTGGTCACCCAAGAGCTCTGATGGAGATGTACAAGGAGATTAATGTTGTTTTCATGCCTGCTAACACAACATCCATTCTGCAGCCCATGGATCAAGGAGTAATTTCGACTTTCAAGTCTTATTATTTAAGAAATACATTTTATAAGGCTATAGCTGCCATAGATAGTGATTCCTCTGATGGATCTGGGCAAAGTAAATTGAAAACCTTCTGGAAAGGATTCACCATTCTAGATGCCATTAAGAACATTCGTGATTCATGGGAGGAGGTCAAAATATCAACATTAACAGGAGTTTGGAAGAAGTTGATTCCAACCCTCATGGATGACTTTGAGGGGTTYAAGACTTCAGTGGAGGAAGTAACTGCAGATGTGGTGGAAATAGCAAGAGAACTAGAATTAGAAGTGGAGCCTGAAGATGTGACTGAATTGCTGCAATCTCATGATAAAACTTGAACGGATGAGGAGTTGCTTCTTATGGATGAGCAAAGAAAGTGGTTTCTTGAGATGGAATCTACTCCTGGTGAAGATGCTGTGAACATTGTTGAAATGACAACAAAGGATTTAGAATATTACATAAACTTAGTTGATAAAGCAGCGGCAGGGTTTGAGAGGATTGACTCCAATTTTGAAAGAAGTTCTACTGTGGGTAAAATGCTGTCAAACAGCATCACATGCTACAGAGAAATCTTTCATGAAAGGAAGAGTCAATTGATGCGGCAAACTTCATTGTTGTCTTATTTTAAGAAATTGCCACAGCCACCCCAACCTTCAGCAACCACCACCCTGATCAGTCAGCAGCCATCAACATCAAGGCAAGACCCTCCACCAGCAAAAAGATTACGACTCGCTGAAGGCTCAGATGATCGTTAGCATTTTTTAGCAATAAAGTATTTTTAAATTAAGGTATGTACATTGTTTTTTAGACATAATGCTATTGCACACTTAATAGACTACAGTATAGCGTAAACATAACTTTTATATGCACTGGGAAACCAAAAAATTCGTGTGACTCGCTTTATTGCGATATTCGCTTTATTGCGGTGGTCTGGAACCGAACCCGCAATATCTCCGAGGTATGCCTG >Tigger2 Autonomous DNA transposon CAGTTGACCCTTGAACAACACGGGTTTGAACTGCGCGGGTCCACTTATACGTGRATTTTTTYCAATAAATATATTGGAAAAAWTTYTGGAGATTTGCAACAATTTGAAAAAACTCGCAGACGAACCGCGTAGCCTAGAAATATTGAAAAAATTAAGAAAAAGGTATGTCATGAATGTATAAAATATATGTAGATACTAGTCTATTTTATCATTTACTACCATAAAATAYACACAAANCTATTATAAAAAGTTAAAATTTATCAAAACTTAYAYAYAYACTTACAGACTGTACGTGGTACCATTCGAAGTNGAGAGAAATATAAACAAACNTAAAGATGCAGTATTAAATCACAACTGCGTAAAATTAACTGTAGTACATACTATACTACCGTAATAATTTTGTAGCCACCTCCTGTTGCTATTGCAGCGAGCTCAAGTGTTGTGAGTACCTGCTTAAAACGCTSKGTGATGCTAATCATCTCCGCGCGAGCAGTTYATCTCTCCAGTAAATTGCGTATTGCAGTAAAAAGTGATCTCTCACGGTTCTCGCGTATTTTTCATTGTGTTTARTGCAATATTGTAAACCTTGAATAACACCATGGGACCCATACGAAGTGCCACTAGTGATGCTGGAAGTGCTCCCAAGAAGCAGAGAAAAGTCATGACATTACAAGAAAAAGTTGAATTGCTTGATATGTACCATAGATTGAGGTCTGCAGCTGGGGTTGCCTGCCATTTCAAGAYAGATGAATCCGGCGTAAGGACCGTTGTMAAAAAAGAAAAGGAAATTCGTGAARACCGTCGCTGCAGTTATGCCCAGCAGGCACAAAAACTTGTNACTTTTTGCGAAATACCTTTTTATGTTGTATTGAAAATGCAGCTTTTNTGTGGGTGCAGGATTGCTATAAGAAAGGMATACCTANAGACTCTAATATGATTAGAGAAAAAGCGAAGTCATTATGTGANNACTTAAAGCAAAARRAAGATGAMRGATCTAAAGCTGGANAATTTAATGCCAGCAAAKGATGGTTTGACAATTTTAGAAAGARGTWTGGCTTAAAAAATKTCAAGAKAACAGGAGAAGCASYTTCTGCTGACCAAGAGACAGCAGACGAGTTNCCAGATGCCATTAACAAAATCATTGAGGAGAAAGGATATCTGCCTGAACAGGTTTTTAATGCAGATGARAGTGCCCTATTCTGGGGGGAAAAAATGCCACAAAGRACATTTATTAGTAAGNAAGAGAAGCGAGCACCAGGATTTAAGGCARGAAGGGATAGGCTAACTCTACTGTTTTGTGCAAATGCAGTTAGGTTTATGATCAGGACTGCCCTTATCTATAAAGCTGCTAACCCCCGAGCCTTGAAGGGAAAAGATAAACGCCAGCTGCCAGTCTTTTGGTTGTACAACAAGAAGGCCTGGACAACGAGAACCCTTTTTCTGGATTGGTTCCATTKATGCTTTGTCCCTGAWGTCAGGAAGTACCTTGCCAGTAAGRGACTGCCTTTTAAAGTTCTTTTGATATTGGACAATGCCCYTGGCCACCCAGAACCCCATGAGTTCAACACCGAAGGTGTCGAAGTGGTCTACTTGCCCCCAAACACAACGTCTCTAATTCAGCCTCTAGATCAGGGGGTCATAAGGACCTTTAAGGCTCATTACACACGGTACTCTATGGAAAGGATTGTCAACGCTATGGAAGAGAACCCCGATAGARAGAACATCATGAAAGTCTGGAAGGATTACACCATTGAAGATGCCATTGTTRTTATAGAAAAAGCCGTGAAAGCCATCAAGCCCAAAACAGTAAATTCCTGCTGGAGAAAACTGTGTCCAGATGTTGTACATGACTTCACAGGATTTACGACAGAGCCAATCAAGGAAATCATGAAAGAGATTGTGGATATGGCAAAAAAGTTGGGGGGTAAAGGGTTTCAAGATGCGAATCTTGGAGAAATTCAAGAGCWAATAGACACCACACCAGAGGAATTAACAGAAGACGACTTGATGGAGATGAGTGCTTCCGAACCAGTGCCAGACGATGAGGAAGAAGACATNGAAGAAGCAGTGCCAGAAAACAAATTGACATTAGACAATCTRGGCAGAAGGGTTCCAATTATTCAAGACTGCTTTTGACTTCTTTTACAACATGGACCCTTCTATGATACGGGCACTGAAACTAAAGCAAACAGTGGAAGAAGGATTGGTAYTATACAGAAACATTTTTAGAGAAATGAAAAGGCAAAAAYRTCAGACAGAAATTACGATGTATTTCCGTAAAGTTACACCGAGTGTGCCTGCCTCTCCTGCCTCCCCTTCCACCTCCTCCACCTCTTCCACCTCTGCCACCCTGAGACAGCAAGACCAACCCCTCCTCCTCCTCAGCCTWCTCAATGTGAAGACGACGAGGATGAAGACCTTTATGATGATCCACTTCCACTTAATGAATAGTAAATATATTTTCTCTTCCTTATGATTTTCTTAATAACATTTTCTTTTCTCTAGCTTACTTTATTGTAAGAATACGGTATATAATACATATAACATACAAAATATGTGTTAATCGACTGTTTATGTTATCGGTAAGGCTTCCAGTCAACAGTAGGCTATTAGTAGTTAAGTTTTKGGGGAGTCAAAAGTTATACGTGGATTTTTNACTGCGCGGGGGGTCAGCGCCCCTAACCCCCGCGTTGTTCAAGGGTCAACTG >PTR5 Human PTR5 mRNA for repetitive sequence GAATTCCGAAGCTTTGTTCTTTAGCTCTTTGCAATAAATCTTGCTGCTGCTCACTCTTTGGGTCCACACTGCCTTTACGAGCTGTAACACTCACCGCGAAGGTCTGCAGCTTCACTCCTGAAGCCAGTGAGACCACGAACCCACCAGGAGGAACAAACAACTCCAGATGTGCTGCCTTAAGAGCTGTAACACTCACCGCGAAGGTCTGCAGCTTCAGTCCTAAGCCAGCAAGACCACGAACCCACCAGAAGGAAGAAACTCTGAACACATCCAAACATCAGAAGGAACAAACTCTGGACATGCCGCCTTTAAGAACTGTGACACTCACCACGAGGGTCCGTGGCTTCATTCTTGAAGTCAGTGAGACCAAGAACCCACCAATTCCAGACACATTTTGGCAACCACAAAGGGACTATCACCTATTGCCAAGCGGTGAGACTATCGCCAAGTGGTGAGACCATCGTCTATCGCTGAGCAGCAAAACAATCGCCTATCGCCAAGCAAATCGAAGCCATCAAGCTACAGATGGTCTTACAAATGGAACCCCAAATGAGTTCAACTAACAACTTCTACCGAGGACCCCTGGACCGACCCGCTGGCACTTCCACTGGCCTAGAGAGTTCCCCCTTGAGACACTACAACTGCAGGGCCCCTTCTTCACTTCTATCCAGCAGGAAGTAGCTAGAGCAGTCATCGGCCAAATTCCCAACAGCAGTTGGGGTGTCCTGTTTAGAGGGGGGATTGAGAGGTGGCAGTGTGCTGGCAGCCCTCGCAGCCCTCGCTCACTCTTGGCTCCTCCTCGGCCTTGGCGCCCACTCTGGCCATGCTTAAGGAGCCCTTCAGCCTGCCACTGCACTGTGGGAACACTGGCCAAGGCCAGAGCCAGCTCACTCAGCTTGTGGGGAAGTGTGGAGGGAGAGGCGCAGGCGGGAACTGGGGCTCGATGCGGCGCTTGCGGGCCAGCGTGAGTTCCGGTGGTTGGTGGCTTGGCGGGCCCCAGACTCGGAGCGGCTGGCCTGCCCTGCTGGCCCCAGGCAGTGAGGGGCTTAGCACCCAGGCCAGCAGCTGTGGATGGTGCGCTGAGTCTCCCAGCAGTGCTGGCCCACCCCACCAGCACTGCACTCGATTTCTCGCCAGGCCTTAGCTGCCTCCCCGCAGGCAGGCCTTGGGACCTGCAGCCCACCATGCCTGAGTCTCCCCTGCTGCCGCCGTGGGCTCCTGCGTGGCCCAAGCCTCCCCGACGAGCACTGCCCCCTGCTCCCCGGTGCCTGGTCCCATCGACCCCAAGGGCTGAGGAGTGCAGGTGCATGCTGCGGGACTGGCAGGCAGCTCCACCTGCGGCCCCATGCAGGAAGCCAGCTGGGCTCCTGAGTCTGGTGGGGACTTGGAGAATTTTTATGTCTAGCTAAGGGATTGTAAATACACCAATCAGCACTCTGTATCTAGCTCAAGGATTGTAAATACACCAATCAGCACCCTGTGTCTAGCTCAGGGTTTGTCAATGCACCAATCGGCACTCTGTATCTAGTTAATCTGGTGGGGAGTTGGAGAATCTTTATGTCTAGCTAAGGGATTGTAAATACACCAATCAGCACTCTGTATCTAGCTCAAGGTTTGTAAACACACCAATCAGAACCCTGTGTCTAGCTCAGGGTTTGTGAATGCACCAATCAGCACTCTGTATCTAGTTAATCTGGTGGGGACTTGGAGAATCTTTATGTCTAGCTAAGGGATTGTGAATGCACCAATCGGCACTCTGTATCTAGCTCAAGGTTGTAAATGGACCAATCAGCACTCTATGTCTAGCTCAGTGTTTGTAAATACACCAATCGACACTCTATCTAGCTAATCTAGTGGGGACGTGGAGAACTTTTGTGTCTAACTCAGGGATTGTAAATGCACCAATCAGAACCCTGTCAAAATGGACCAATAGCTCTCTGTAAAACAGACTGACTTTCTGTAAAATGGACCAATCAGCAGGATGTGGGTGGGGCCAGATAAGAGAAGAAAAGCAGGCTGCCTGAGCCAGCAGTGGCAACCCGCTTGGGTCCCCTTCCACACTGTGGAAGCTTTGTTCTTTCGCTCTTTGCAATAAATCTTGCTGCTGCTCACTCTTTGGGTCCACACTGCCTTTATGAGCTGTAACACTCACCGCGAAGGTCTGCAGCTTCATCTGAAGCAGCGAGACACGAACCCACCAGGAGGAACAAACAACTCCGATGCGCTGCCTTAAGAGCTGTAACACCGCGAGGTCTCGAGCTTCACTCCTGAGCCAGCAAGACCACAAACCCACCAGAAGGAAGAAACTCAGGAAAACAGCCGAACATCAGAAGGAATAAACTCTGGACACACCACCTTTAAGAACTGTGACACTCGCTGCGAGCGTCTGTGGCTTCATTCTTGAAGTCAGTGAGACCAAGAACCCACCAATTCTGGAC >PAB Human pseudoautosomal boundary-like sequence. CCTTACCAGCCCCCTACTGTGGCAGGCCAGGTCCCACTAACACAGGCCTCCATAACAACTGTTTCAGCTCTGACTGAGTGGTTAAGTTAAATACTAAAAGCCGAGAGAGCCAGTCCCCTTATACAGAGGCTGGAATGTAACAAAAGCCCACCAAGAGTTTTGCCTAGGCCTTTCCTGGGCCTTGAAGCATGACAAAATAACAAAAGAATTCTTAACAGAATCTATTTAGGATTAAACAAGTTTTACTGGGGGTTCTGAAGAAACTCCCCAGTCCTCCACAAACAAGTTTACTGGGGTCTGAAAGAACTCCCCAAACCTCCATGATTTAGCAGGAGACAAGATAAGGGTAATCACCCCAGCACCTGGACCCAGCTAGATTTAGTCAATTTACTGAGGCTACAAAGGAAGGTCTTCAGGACTCAGACCTCAGTTATAGATTAGAAGAAGTTAATCACTTATGTCTTTAGATGATTGCACACTTACACATAGACATATAGCTTAGAAGGTGTATAAGCTCTGGAAAACTTTGTAATTTTGAGTTGGTCTGGTGATATTTTCCAGGCCTTCTCCCTATACCCGGTTACAGAAATAAAAACTTTCTTCCTCCCCAGTTCATCTGCATCTTGTTAATGGGCTGCCAGAAATAGCAGCCCAACCCTCAGTTAGGTCTGGGAACACTACCCCCCAACACACACACACACAC >TARE Transposable element GAATTCCGCGCCCGTCCCGTCGCCGCCGCCGCCGCCGCAGACCCCTCGGTCTTGCTATGTCGAGCTCACCCGTGAAGCGTCAGAGGATGGAGTCCGCGCTGGACCAGCTCAAGCAGTTCACCACCGTGGTGGCCGACACGGGCGACTTCCACGCCATCGACGAGTACAAGCCCCAGGATGCTACCACCAACCCGTCCCTGATCCTGGCCGCAGCACAGATGCCCGCTTACCAGGAGCTGGTGGAGGAGGCGATTGCCTATGGCCGGAAGCTGGGCGGGTCACAAGAGGACCAGATTAAAAATGCTATTGATAAACTTTTTGTGTTGTTTGGAGCAGAAATACTAAAGAAGATTCCGGGCCGAGTATCCACAGAAGTAGACGCAAGGCTCTCCTTTGATAAAGATGCGATGGTGGCCAGAGCCAGGCGGCTCATCGAGCTCTACAAGGAAGCTGGGATCAGCAAGGACCGA >A Primate simple repeat pattern AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA >C Primate simple repeat pattern CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC >AC Primate simple repeat pattern ACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACA >AT Primate simple repeat pattern ATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA >AG Primate simple repeat pattern AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA >CG Primate simple repeat pattern CGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGC >GCC Primate simple repeat pattern GCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCC >TAA Primate simple repeat pattern TAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAA >CAA Primate simple repeat pattern CAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAA >GCA Primate simple repeat pattern GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA >CTA Primate simple repeat pattern CTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTACTA >GGA Primate simple repeat pattern GGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGA >GAA Primate simple repeat pattern GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA >TCA Primate simple repeat pattern TCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCA >CCA Primate simple repeat pattern CCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA >TAGA Primate simple repeat pattern TAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGAT >CAAA Primate simple repeat pattern CAAACAAACAAACAAACAAACAAACAAACAAACAAACAAACAAACAAACAAACAAACAAACAAACAAAC >TAAA Primate simple repeat pattern TAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAAT >GAAA Primate simple repeat pattern GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG >TCCA Primate simple repeat pattern TCCATCCATCCATCCATCCATCCATCCATCCATCCATCCATCCATCCATCCATCCATCCATCCATCCAT >CGAA Primate simple repeat pattern CGAACGAACGAACGAACGAACGAACGAACGAACGAACGAACGAACGAACGAACGAACGAACGAACGAAC >TGAA Primate simple repeat pattern TGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGAAT >CGGA Primate simple repeat pattern CGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGAC >GGAA Primate simple repeat pattern GGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAG >TACA Primate simple repeat pattern TACATACATACATACATACATACATACATACATACATACATACATACATACATACATACATACATACAT >TCAA Primate simple repeat pattern TCAATCAATCAATCAATCAATCAATCAATCAATCAATCAATCAATCAATCAATCAATCAATCAATCAAT >GACA Primate simple repeat pattern GACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAG >CCTA Primate simple repeat pattern CCTACCTACCTACCTACCTACCTACCTACCTACCTACCTACCTACCTACCTACCTACCTACCTACCTAC >GCCC Primate simple repeat pattern GCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCG >TTAA Primate simple repeat pattern TTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAAT >GGGA Primate simple repeat pattern GGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAG >GGCA Primate simple repeat pattern GGCAGGCAGGCAGGCAGGCAGGCAGGCAGGCAGGCAGGCAGGCAGGCAGGCAGGCAGGCAGGCAGGCAG >GCCA Primate simple repeat pattern GCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAG >CAAAA Primate simple repeat pattern CAAAACAAAACAAAACAAAACAAAACAAAACAAAACAAAACAAAACAAAACAAAACAAAACAAAACAAA >TGGAA Primate simple repeat pattern TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGA >TAAAA Primate simple repeat pattern TAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAA >GCCCA Primate simple repeat pattern GCCCAGCCCAGCCCAGCCCAGCCCAGCCCAGCCCAGCCCAGCCCAGCCCAGCCCAGCCCAGCCCAGCCC >GCTCA Primate simple repeat pattern GCTCAGCTCAGCTCAGCTCAGCTCAGCTCAGCTCAGCTCAGCTCAGCTCAGCTCAGCTCAGCTCAGCTC >GAAAA Primate simple repeat pattern GAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAA >TACAA Primate simple repeat pattern TACAATACAATACAATACAATACAATACAATACAATACAATACAATACAATACAATACAATACAATACA >CTCCA Primate simple repeat pattern CTCCACTCCACTCCACTCCACTCCACTCCACTCCACTCCACTCCACTCCACTCCACTCCACTCCACTCC >TCTAA Primate simple repeat pattern TCTAATCTAATCTAATCTAATCTAATCTAATCTAATCTAATCTAATCTAATCTAATCTAATCTAATCTA >TAGAA Primate simple repeat pattern TAGAATAGAATAGAATAGAATAGAATAGAATAGAATAGAATAGAATAGAATAGAATAGAATAGAATAGA >GGAGA Primate simple repeat pattern GGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGAG >TTAAA Primate simple repeat pattern TTAAATTAAATTAAATTAAATTAAATTAAATTAAATTAAATTAAATTAAATTAAATTAAATTAAATTAA >GCCCC Primate simple repeat pattern GCCCCGCCCCGCCCCGCCCCGCCCCGCCCCGCCCCGCCCCGCCCCGCCCCGCCCCGCCCCGCCCCGCCC >GCGCA Primate simple repeat pattern GCGCAGCGCAGCGCAGCGCAGCGCAGCGCAGCGCAGCGCAGCGCAGCGCAGCGCAGCGCAGCGCAGCGC >GGGGA Primate simple repeat pattern GGGGAGGGGAGGGGAGGGGAGGGGAGGGGAGGGGAGGGGAGGGGAGGGGAGGGGAGGGGAGGGGAGGGG >GGCCC Primate simple repeat pattern GGCCCGGCCCGGCCCGGCCCGGCCCGGCCCGGCCCGGCCCGGCCCGGCCCGGCCCGGCCCGGCCCGGCC >TCCCA Primate simple repeat pattern TCCCATCCCATCCCATCCCATCCCATCCCATCCCATCCCATCCCATCCCATCCCATCCCATCCCATCCC >CCCCA Primate simple repeat pattern CCCCACCCCACCCCACCCCACCCCACCCCACCCCACCCCACCCCACCCCACCCCACCCCACCCCACCCC >CCCTAA Primate simple repeat pattern CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCC >CCCGAA Primate simple repeat pattern CCCGAACCCGAACCCGAACCCGAACCCGAACCCGAACCCGAACCCGAACCCGAACCCGAACCCGAACCC >TATACA Primate simple repeat pattern TATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATAT >TCACCA Primate simple repeat pattern TCACCATCACCATCACCATCACCATCACCATCACCATCACCATCACCATCACCATCACCATCACCATCA >CCCCAA Primate simple repeat pattern CCCCAACCCCAACCCCAACCCCAACCCCAACCCCAACCCCAACCCCAACCCCAACCCCAACCCCAACCC >CAAAAA Primate simple repeat pattern CAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAA >CCCTCA Primate simple repeat pattern CCCTCACCCTCACCCTCACCCTCACCCTCACCCTCACCCTCACCCTCACCCTCACCCTCACCCTCACCC >GCCCCC Primate simple repeat pattern GCCCCCGCCCCCGCCCCCGCCCCCGCCCCCGCCCCCGCCCCCGCCCCCGCCCCCGCCCCCGCCCCCGCC >GAAAAA Primate simple repeat pattern GAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAA >GGAGAA Primate simple repeat pattern GGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGA >CCCCCA Primate simple repeat pattern CCCCCACCCCCACCCCCACCCCCACCCCCACCCCCACCCCCACCCCCACCCCCACCCCCACCCCCACCC >TAAAAA Primate simple repeat pattern TAAAAATAAAAATAAAAATAAAAATAAAAATAAAAATAAAAATAAAAATAAAAATAAAAATAAAAATAA >GGGAGA Primate simple repeat pattern GGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGG >GCCCCA Primate simple repeat pattern GCCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCC >GGGGGA Primate simple repeat pattern GGGGGAGGGGGAGGGGGAGGGGGAGGGGGAGGGGGAGGGGGAGGGGGAGGGGGAGGGGGAGGGGGAGGG >GAGACA Primate simple repeat pattern GAGACAGAGACAGAGACAGAGACAGAGACAGAGACAGAGACAGAGACAGAGACAGAGACAGAGACAGAG >A Primate simple repeat pattern TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT >C Primate simple repeat pattern GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >AC Primate simple repeat pattern TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT >AT Primate simple repeat pattern TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAT >AG Primate simple repeat pattern TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT >CG Primate simple repeat pattern GCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCG >GCC Primate simple repeat pattern GGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGC >TAA Primate simple repeat pattern TTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTA >CAA Primate simple repeat pattern TTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTG >GCA Primate simple repeat pattern TGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGC >CTA Primate simple repeat pattern TAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAG >GGA Primate simple repeat pattern TCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCC >GAA Primate simple repeat pattern TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC >TCA Primate simple repeat pattern TGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGA >CCA Primate simple repeat pattern TGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG >TAGA Primate simple repeat pattern ATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTA >CAAA Primate simple repeat pattern GTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTG >TAAA Primate simple repeat pattern ATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTA >GAAA Primate simple repeat pattern CTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTC >TCCA Primate simple repeat pattern ATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGA >CGAA Primate simple repeat pattern GTTCGTTCGTTCGTTCGTTCGTTCGTTCGTTCGTTCGTTCGTTCGTTCGTTCGTTCGTTCGTTCGTTCG >TGAA Primate simple repeat pattern ATTCATTCATTCATTCATTCATTCATTCATTCATTCATTCATTCATTCATTCATTCATTCATTCATTCA >CGGA Primate simple repeat pattern GTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCCG >GGAA Primate simple repeat pattern CTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC >TACA Primate simple repeat pattern ATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTA >TCAA Primate simple repeat pattern ATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGA >GACA Primate simple repeat pattern CTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTCTGTC >CCTA Primate simple repeat pattern GTAGGTAGGTAGGTAGGTAGGTAGGTAGGTAGGTAGGTAGGTAGGTAGGTAGGTAGGTAGGTAGGTAGG >GCCC Primate simple repeat pattern CGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGC >TTAA Primate simple repeat pattern ATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAATTAA >GGGA Primate simple repeat pattern CTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCC >GGCA Primate simple repeat pattern CTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCC >GCCA Primate simple repeat pattern CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGC >CAAAA Primate simple repeat pattern TTTGTTTTGTTTTGTTTTGTTTTGTTTTGTTTTGTTTTGTTTTGTTTTGTTTTGTTTTGTTTTGTTTTG >TGGAA Primate simple repeat pattern TCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCA >TAAAA Primate simple repeat pattern TTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTA >GCCCA Primate simple repeat pattern GGGCTGGGCTGGGCTGGGCTGGGCTGGGCTGGGCTGGGCTGGGCTGGGCTGGGCTGGGCTGGGCTGGGC >GCTCA Primate simple repeat pattern GAGCTGAGCTGAGCTGAGCTGAGCTGAGCTGAGCTGAGCTGAGCTGAGCTGAGCTGAGCTGAGCTGAGC >GAAAA Primate simple repeat pattern TTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTC >TACAA Primate simple repeat pattern TGTATTGTATTGTATTGTATTGTATTGTATTGTATTGTATTGTATTGTATTGTATTGTATTGTATTGTA >CTCCA Primate simple repeat pattern GGAGTGGAGTGGAGTGGAGTGGAGTGGAGTGGAGTGGAGTGGAGTGGAGTGGAGTGGAGTGGAGTGGAG >TCTAA Primate simple repeat pattern TAGATTAGATTAGATTAGATTAGATTAGATTAGATTAGATTAGATTAGATTAGATTAGATTAGATTAGA >TAGAA Primate simple repeat pattern TCTATTCTATTCTATTCTATTCTATTCTATTCTATTCTATTCTATTCTATTCTATTCTATTCTATTCTA >GGAGA Primate simple repeat pattern CTCCTCTCCTCTCCTCTCCTCTCCTCTCCTCTCCTCTCCTCTCCTCTCCTCTCCTCTCCTCTCCTCTCC >TTAAA Primate simple repeat pattern TTAATTTAATTTAATTTAATTTAATTTAATTTAATTTAATTTAATTTAATTTAATTTAATTTAATTTAA >GCCCC Primate simple repeat pattern GGGCGGGGCGGGGCGGGGCGGGGCGGGGCGGGGCGGGGCGGGGCGGGGCGGGGCGGGGCGGGGCGGGGC >GCGCA Primate simple repeat pattern GCGCTGCGCTGCGCTGCGCTGCGCTGCGCTGCGCTGCGCTGCGCTGCGCTGCGCTGCGCTGCGCTGCGC >GGGGA Primate simple repeat pattern CCCCTCCCCTCCCCTCCCCTCCCCTCCCCTCCCCTCCCCTCCCCTCCCCTCCCCTCCCCTCCCCTCCCC >GGCCC Primate simple repeat pattern GGCCGGGCCGGGCCGGGCCGGGCCGGGCCGGGCCGGGCCGGGCCGGGCCGGGCCGGGCCGGGCCGGGCC >TCCCA Primate simple repeat pattern GGGATGGGATGGGATGGGATGGGATGGGATGGGATGGGATGGGATGGGATGGGATGGGATGGGATGGGA >CCCCA Primate simple repeat pattern GGGGTGGGGTGGGGTGGGGTGGGGTGGGGTGGGGTGGGGTGGGGTGGGGTGGGGTGGGGTGGGGTGGGG >CCCTAA Primate simple repeat pattern GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG >CCCGAA Primate simple repeat pattern GGGTTCGGGTTCGGGTTCGGGTTCGGGTTCGGGTTCGGGTTCGGGTTCGGGTTCGGGTTCGGGTTCGGG >TATACA Primate simple repeat pattern ATATGTATATGTATATGTATATGTATATGTATATGTATATGTATATGTATATGTATATGTATATGTATA >TCACCA Primate simple repeat pattern TGATGGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGGTGA >CCCCAA Primate simple repeat pattern GGGTTGGGGTTGGGGTTGGGGTTGGGGTTGGGGTTGGGGTTGGGGTTGGGGTTGGGGTTGGGGTTGGGG >CAAAAA Primate simple repeat pattern TTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTG >CCCTCA Primate simple repeat pattern GGGTGAGGGTGAGGGTGAGGGTGAGGGTGAGGGTGAGGGTGAGGGTGAGGGTGAGGGTGAGGGTGAGGG >GCCCCC Primate simple repeat pattern GGCGGGGGCGGGGGCGGGGGCGGGGGCGGGGGCGGGGGCGGGGGCGGGGGCGGGGGCGGGGGCGGGGGC >GAAAAA Primate simple repeat pattern TTCTTTTTCTTTTTCTTTTTCTTTTTCTTTTTCTTTTTCTTTTTCTTTTTCTTTTTCTTTTTCTTTTTC >GGAGAA Primate simple repeat pattern TCCTTCTCCTTCTCCTTCTCCTTCTCCTTCTCCTTCTCCTTCTCCTTCTCCTTCTCCTTCTCCTTCTCC >CCCCCA Primate simple repeat pattern GGGTGGGGGTGGGGGTGGGGGTGGGGGTGGGGGTGGGGGTGGGGGTGGGGGTGGGGGTGGGGGTGGGGG >TAAAAA Primate simple repeat pattern TTATTTTTATTTTTATTTTTATTTTTATTTTTATTTTTATTTTTATTTTTATTTTTATTTTTATTTTTA >GGGAGA Primate simple repeat pattern CCCTCTCCCTCTCCCTCTCCCTCTCCCTCTCCCTCTCCCTCTCCCTCTCCCTCTCCCTCTCCCTCTCCC >GCCCCA Primate simple repeat pattern GGCTGGGGCTGGGGCTGGGGCTGGGGCTGGGGCTGGGGCTGGGGCTGGGGCTGGGGCTGGGGCTGGGGC >GGGGGA Primate simple repeat pattern CCCTCCCCCTCCCCCTCCCCCTCCCCCTCCCCCTCCCCCTCCCCCTCCCCCTCCCCCTCCCCCTCCCCC >GAGACA Primate simple repeat pattern CTCTGTCTCTGTCTCTGTCTCTGTCTCTGTCTCTGTCTCTGTCTCTGTCTCTGTCTCTGTCTCTGTCTC primer3-2.3.6/src/gpl-2.0.txt0000664000077300007730000004310312231156245014466 0ustar ioanaioana GNU GENERAL PUBLIC LICENSE Version 2, June 1991 Copyright (C) 1989, 1991 Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed. Preamble The licenses for most software are designed to take away your freedom to share and change it. By contrast, the GNU General Public License is intended to guarantee your freedom to share and change free software--to make sure the software is free for all its users. This General Public License applies to most of the Free Software Foundation's software and to any other program whose authors commit to using it. (Some other Free Software Foundation software is covered by the GNU Lesser General Public License instead.) You can apply it to your programs, too. When we speak of free software, we are referring to freedom, not price. Our General Public Licenses are designed to make sure that you have the freedom to distribute copies of free software (and charge for this service if you wish), that you receive source code or can get it if you want it, that you can change the software or use pieces of it in new free programs; and that you know you can do these things. To protect your rights, we need to make restrictions that forbid anyone to deny you these rights or to ask you to surrender the rights. These restrictions translate to certain responsibilities for you if you distribute copies of the software, or if you modify it. For example, if you distribute copies of such a program, whether gratis or for a fee, you must give the recipients all the rights that you have. You must make sure that they, too, receive or can get the source code. And you must show them these terms so they know their rights. We protect your rights with two steps: (1) copyright the software, and (2) offer you this license which gives you legal permission to copy, distribute and/or modify the software. Also, for each author's protection and ours, we want to make certain that everyone understands that there is no warranty for this free software. If the software is modified by someone else and passed on, we want its recipients to know that what they have is not the original, so that any problems introduced by others will not reflect on the original authors' reputations. Finally, any free program is threatened constantly by software patents. We wish to avoid the danger that redistributors of a free program will individually obtain patent licenses, in effect making the program proprietary. To prevent this, we have made it clear that any patent must be licensed for everyone's free use or not licensed at all. The precise terms and conditions for copying, distribution and modification follow. GNU GENERAL PUBLIC LICENSE TERMS AND CONDITIONS FOR COPYING, DISTRIBUTION AND MODIFICATION 0. This License applies to any program or other work which contains a notice placed by the copyright holder saying it may be distributed under the terms of this General Public License. The "Program", below, refers to any such program or work, and a "work based on the Program" means either the Program or any derivative work under copyright law: that is to say, a work containing the Program or a portion of it, either verbatim or with modifications and/or translated into another language. (Hereinafter, translation is included without limitation in the term "modification".) Each licensee is addressed as "you". Activities other than copying, distribution and modification are not covered by this License; they are outside its scope. The act of running the Program is not restricted, and the output from the Program is covered only if its contents constitute a work based on the Program (independent of having been made by running the Program). Whether that is true depends on what the Program does. 1. You may copy and distribute verbatim copies of the Program's source code as you receive it, in any medium, provided that you conspicuously and appropriately publish on each copy an appropriate copyright notice and disclaimer of warranty; keep intact all the notices that refer to this License and to the absence of any warranty; and give any other recipients of the Program a copy of this License along with the Program. You may charge a fee for the physical act of transferring a copy, and you may at your option offer warranty protection in exchange for a fee. 2. You may modify your copy or copies of the Program or any portion of it, thus forming a work based on the Program, and copy and distribute such modifications or work under the terms of Section 1 above, provided that you also meet all of these conditions: a) You must cause the modified files to carry prominent notices stating that you changed the files and the date of any change. b) You must cause any work that you distribute or publish, that in whole or in part contains or is derived from the Program or any part thereof, to be licensed as a whole at no charge to all third parties under the terms of this License. c) If the modified program normally reads commands interactively when run, you must cause it, when started running for such interactive use in the most ordinary way, to print or display an announcement including an appropriate copyright notice and a notice that there is no warranty (or else, saying that you provide a warranty) and that users may redistribute the program under these conditions, and telling the user how to view a copy of this License. (Exception: if the Program itself is interactive but does not normally print such an announcement, your work based on the Program is not required to print an announcement.) These requirements apply to the modified work as a whole. If identifiable sections of that work are not derived from the Program, and can be reasonably considered independent and separate works in themselves, then this License, and its terms, do not apply to those sections when you distribute them as separate works. But when you distribute the same sections as part of a whole which is a work based on the Program, the distribution of the whole must be on the terms of this License, whose permissions for other licensees extend to the entire whole, and thus to each and every part regardless of who wrote it. Thus, it is not the intent of this section to claim rights or contest your rights to work written entirely by you; rather, the intent is to exercise the right to control the distribution of derivative or collective works based on the Program. In addition, mere aggregation of another work not based on the Program with the Program (or with a work based on the Program) on a volume of a storage or distribution medium does not bring the other work under the scope of this License. 3. You may copy and distribute the Program (or a work based on it, under Section 2) in object code or executable form under the terms of Sections 1 and 2 above provided that you also do one of the following: a) Accompany it with the complete corresponding machine-readable source code, which must be distributed under the terms of Sections 1 and 2 above on a medium customarily used for software interchange; or, b) Accompany it with a written offer, valid for at least three years, to give any third party, for a charge no more than your cost of physically performing source distribution, a complete machine-readable copy of the corresponding source code, to be distributed under the terms of Sections 1 and 2 above on a medium customarily used for software interchange; or, c) Accompany it with the information you received as to the offer to distribute corresponding source code. (This alternative is allowed only for noncommercial distribution and only if you received the program in object code or executable form with such an offer, in accord with Subsection b above.) The source code for a work means the preferred form of the work for making modifications to it. For an executable work, complete source code means all the source code for all modules it contains, plus any associated interface definition files, plus the scripts used to control compilation and installation of the executable. However, as a special exception, the source code distributed need not include anything that is normally distributed (in either source or binary form) with the major components (compiler, kernel, and so on) of the operating system on which the executable runs, unless that component itself accompanies the executable. If distribution of executable or object code is made by offering access to copy from a designated place, then offering equivalent access to copy the source code from the same place counts as distribution of the source code, even though third parties are not compelled to copy the source along with the object code. 4. You may not copy, modify, sublicense, or distribute the Program except as expressly provided under this License. Any attempt otherwise to copy, modify, sublicense or distribute the Program is void, and will automatically terminate your rights under this License. However, parties who have received copies, or rights, from you under this License will not have their licenses terminated so long as such parties remain in full compliance. 5. You are not required to accept this License, since you have not signed it. However, nothing else grants you permission to modify or distribute the Program or its derivative works. These actions are prohibited by law if you do not accept this License. Therefore, by modifying or distributing the Program (or any work based on the Program), you indicate your acceptance of this License to do so, and all its terms and conditions for copying, distributing or modifying the Program or works based on it. 6. Each time you redistribute the Program (or any work based on the Program), the recipient automatically receives a license from the original licensor to copy, distribute or modify the Program subject to these terms and conditions. You may not impose any further restrictions on the recipients' exercise of the rights granted herein. You are not responsible for enforcing compliance by third parties to this License. 7. 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If any portion of this section is held invalid or unenforceable under any particular circumstance, the balance of the section is intended to apply and the section as a whole is intended to apply in other circumstances. It is not the purpose of this section to induce you to infringe any patents or other property right claims or to contest validity of any such claims; this section has the sole purpose of protecting the integrity of the free software distribution system, which is implemented by public license practices. Many people have made generous contributions to the wide range of software distributed through that system in reliance on consistent application of that system; it is up to the author/donor to decide if he or she is willing to distribute software through any other system and a licensee cannot impose that choice. This section is intended to make thoroughly clear what is believed to be a consequence of the rest of this License. 8. If the distribution and/or use of the Program is restricted in certain countries either by patents or by copyrighted interfaces, the original copyright holder who places the Program under this License may add an explicit geographical distribution limitation excluding those countries, so that distribution is permitted only in or among countries not thus excluded. In such case, this License incorporates the limitation as if written in the body of this License. 9. The Free Software Foundation may publish revised and/or new versions of the General Public License from time to time. Such new versions will be similar in spirit to the present version, but may differ in detail to address new problems or concerns. Each version is given a distinguishing version number. If the Program specifies a version number of this License which applies to it and "any later version", you have the option of following the terms and conditions either of that version or of any later version published by the Free Software Foundation. If the Program does not specify a version number of this License, you may choose any version ever published by the Free Software Foundation. 10. If you wish to incorporate parts of the Program into other free programs whose distribution conditions are different, write to the author to ask for permission. For software which is copyrighted by the Free Software Foundation, write to the Free Software Foundation; we sometimes make exceptions for this. Our decision will be guided by the two goals of preserving the free status of all derivatives of our free software and of promoting the sharing and reuse of software generally. NO WARRANTY 11. BECAUSE THE PROGRAM IS LICENSED FREE OF CHARGE, THERE IS NO WARRANTY FOR THE PROGRAM, TO THE EXTENT PERMITTED BY APPLICABLE LAW. EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT HOLDERS AND/OR OTHER PARTIES PROVIDE THE PROGRAM "AS IS" WITHOUT WARRANTY OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM IS WITH YOU. SHOULD THE PROGRAM PROVE DEFECTIVE, YOU ASSUME THE COST OF ALL NECESSARY SERVICING, REPAIR OR CORRECTION. 12. IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING WILL ANY COPYRIGHT HOLDER, OR ANY OTHER PARTY WHO MAY MODIFY AND/OR REDISTRIBUTE THE PROGRAM AS PERMITTED ABOVE, BE LIABLE TO YOU FOR DAMAGES, INCLUDING ANY GENERAL, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE USE OR INABILITY TO USE THE PROGRAM (INCLUDING BUT NOT LIMITED TO LOSS OF DATA OR DATA BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS), EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. END OF TERMS AND CONDITIONS How to Apply These Terms to Your New Programs If you develop a new program, and you want it to be of the greatest possible use to the public, the best way to achieve this is to make it free software which everyone can redistribute and change under these terms. To do so, attach the following notices to the program. It is safest to attach them to the start of each source file to most effectively convey the exclusion of warranty; and each file should have at least the "copyright" line and a pointer to where the full notice is found. Copyright (C) This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA. Also add information on how to contact you by electronic and paper mail. If the program is interactive, make it output a short notice like this when it starts in an interactive mode: Gnomovision version 69, Copyright (C) year name of author Gnomovision comes with ABSOLUTELY NO WARRANTY; for details type `show w'. This is free software, and you are welcome to redistribute it under certain conditions; type `show c' for details. The hypothetical commands `show w' and `show c' should show the appropriate parts of the General Public License. Of course, the commands you use may be called something other than `show w' and `show c'; they could even be mouse-clicks or menu items--whatever suits your program. You should also get your employer (if you work as a programmer) or your school, if any, to sign a "copyright disclaimer" for the program, if necessary. Here is a sample; alter the names: Yoyodyne, Inc., hereby disclaims all copyright interest in the program `Gnomovision' (which makes passes at compilers) written by James Hacker. , 1 April 1989 Ty Coon, President of Vice This General Public License does not permit incorporating your program into proprietary programs. If your program is a subroutine library, you may consider it more useful to permit linking proprietary applications with the library. If this is what you want to do, use the GNU Lesser General Public License instead of this License. primer3-2.3.6/src/format_output.c0000664000077300007730000010324712231156245015730 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009,2010 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of primer3 and the libprimer3 library. Primer3 and the libprimer3 library are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this software (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANYT HEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include #include #include "format_output.h" #include "libprimer3.h" #define FORWARD 1 #define REVERSE -1 static const char *pr_program_name = "Program name is probably primer3_core"; static void format_pairs(FILE *f, const p3_global_settings *pa, const seq_args *sa, const p3retval *retval, const pair_array_t *best_pairs, const char *pr_release, const pr_append_str *combined_retval_err, int explain_flag); static void format_oligos(FILE *, const p3_global_settings *, const seq_args *, const p3retval *retval, const char*, const pr_append_str *combined_retval_err, int explain_flag); static int lib_sim_specified(const p3_global_settings *); static void print_explain(FILE *, const p3_global_settings *, const seq_args *, const p3retval *retval, int, const char *); static void print_pair_info(FILE *, const primer_pair *, const p3_global_settings *); static void print_oligo(FILE *, const char *, const seq_args *, const primer_rec *, int, const p3_global_settings *, const seq_lib*, int); static void print_oligo_header(FILE *, const char *, const int, const int); static void print_pair_array(FILE *, const char*, int, const interval_array_t, const p3_global_settings*, const seq_args*); static void print_2_pair_array(FILE *, const char*, int, const interval_array_t, const interval_array_t, const p3_global_settings*, const seq_args*); static void print_rest(FILE *, const p3_global_settings *, const seq_args *, const pair_array_t *); static int print_seq(FILE *, const p3_global_settings *, const seq_args *, const p3retval *retval, primer_rec *h, const pair_array_t *, int); static void print_seq_lines(FILE *, const char *s, const char *n, int, int, int, const p3_global_settings *); static void print_stat_line(FILE *, const char *, oligo_stats s, int, int, int); static void print_summary(FILE *, const p3_global_settings *, const seq_args *, const pair_array_t *, int); void print_format_output(FILE *f, const int *io_version, const p3_global_settings *pa, const seq_args *sa, const p3retval *retval, const char *pr_release, int explain_flag) { /* A place to put a string containing all error messages */ pr_append_str *combined_retval_err = NULL; combined_retval_err = create_pr_append_str(); if (NULL == combined_retval_err) exit(-2); /* Out of memory */ if (pr_append_new_chunk_external(combined_retval_err, retval->glob_err.data)) exit(-2); if (pr_append_new_chunk_external(combined_retval_err, retval->per_sequence_err.data)) exit(-2); /* Print as primer pairs */ if (retval->output_type == primer_pairs) { format_pairs(f, pa, sa, retval, &retval->best_pairs, pr_release, combined_retval_err, explain_flag); /* Print as primer list */ } else { format_oligos(stdout, pa, sa, retval, pr_release, combined_retval_err, explain_flag); } destroy_pr_append_str(combined_retval_err); } static void format_pairs(FILE *f, const p3_global_settings *pa, const seq_args *sa, const p3retval *retval, const pair_array_t *best_pairs, const char *pr_release, const pr_append_str *combined_retval_err, int explain_flag) { char *warning; int print_lib_sim = lib_sim_specified(pa); primer_rec *h = NULL; PR_ASSERT(NULL != f); PR_ASSERT(NULL != pa); PR_ASSERT(NULL != sa); /* If there are errors, print them and return */ if (!pr_is_empty(combined_retval_err)) { format_error(f, sa->sequence_name, pr_append_str_chars(combined_retval_err)); return; } /* Print the sequence name if it is provided */ if (NULL != sa->sequence_name) fprintf(f, "PRIMER PICKING RESULTS FOR %s\n\n", sa->sequence_name); /* Print if a mispriming libraby was used and which one */ if (pa->p_args.repeat_lib != NULL) fprintf(f, "Using mispriming library %s\n", pa->p_args.repeat_lib->repeat_file); else fprintf(f, "No mispriming library specified\n"); /* Print if a mispriming libraby for the internal oligo * was used and which one */ if ( pa->pick_internal_oligo == 1 ) { if (pa->o_args.repeat_lib != NULL) fprintf(f, "Using internal oligo mishyb library %s\n", pa->o_args.repeat_lib->repeat_file); else fprintf(f, "No internal oligo mishyb library specified\n"); } /* Does the sequence start at position 0 or 1 ? */ fprintf(f, "Using %d-based sequence positions\n", pa->first_base_index); /* Complain if no primers are in the array */ if (best_pairs->num_pairs == 0) fprintf(f, "NO PRIMERS FOUND\n\n"); /* Print out the warings */ if ((warning = p3_get_rv_and_gs_warnings(retval, pa)) != NULL) { fprintf(f, "WARNING: %s\n\n", warning); free(warning); } /* Print the results for the best pair */ print_summary(f, pa, sa, best_pairs, 0); fprintf(f, "\n"); /* Print nicely out the sequence with the best pair */ if (print_seq(f, pa, sa, retval, h, best_pairs, 0)) exit(-2); /* ENOMEM */ /* Print out the alternative pairs */ if (best_pairs->num_pairs > 1 ) print_rest(f, pa, sa, best_pairs); /* Print the primer picking statistics */ if (explain_flag) print_explain(f, pa, sa, retval, print_lib_sim, pr_release); /* Flush the buffers and return */ fprintf(f, "\n\n"); if (fflush(f) == EOF) { perror("fflush(f) failed"); exit(-1); } } /* Prints out the results of a primer pair */ static void print_summary(FILE *f, const p3_global_settings *pa, const seq_args *sa, const pair_array_t *best_pairs, int num) { int seq_len = strlen(sa->sequence); int print_lib_sim = lib_sim_specified(pa); primer_pair *p; p = best_pairs->pairs + num; if (best_pairs->num_pairs > 0) { /* * If the following format changes, also change the format in * print_oligo. */ print_oligo_header(f, "OLIGO", print_lib_sim, pa->thermodynamic_oligo_alignment); print_oligo(f, "LEFT PRIMER", sa, p->left, FORWARD, pa, pa->p_args.repeat_lib, print_lib_sim); print_oligo(f, "RIGHT PRIMER", sa, p->right, REVERSE, pa, pa->p_args.repeat_lib, print_lib_sim); if ( pa->pick_internal_oligo == 1 ) print_oligo(f, "INTERNAL OLIGO", sa, p->intl, FORWARD, pa, pa->o_args.repeat_lib, print_lib_sim); } fprintf(f, "SEQUENCE SIZE: %d\n", seq_len); fprintf(f, "INCLUDED REGION SIZE: %d\n\n", sa->incl_l); if (best_pairs->num_pairs > 0) print_pair_info(f, p, pa); print_pair_array(f, "TARGETS", sa->tar2.count, sa->tar2.pairs, pa, sa); print_pair_array(f, "EXCLUDED REGIONS", sa->excl2.count, sa->excl2.pairs, pa, sa); print_pair_array(f, "INTERNAL OLIGO EXCLUDED REGIONS", sa->excl_internal2.count, sa->excl_internal2.pairs, pa, sa); print_2_pair_array(f, "PAIR_OK_REGIONS", sa->ok_regions.count, sa->ok_regions.left_pairs, sa->ok_regions.right_pairs, pa, sa); } /* Print column headers for lines printed by print_oligo(). */ static void print_oligo_header(FILE *f, const char *s, const int print_lib_sim, const int thermodynamic_oligo_alignment) { if(thermodynamic_oligo_alignment==0) fprintf(f, "%-16s start len tm gc%% any 3' %sseq\n", s, print_lib_sim ? " rep " : ""); else fprintf(f, "%-16s start len tm gc%% any_th 3'_th hairpin %sseq\n", s, print_lib_sim ? " rep " : ""); } /* Print the line with the parameters */ static void print_oligo(FILE *f, const char *title, const seq_args *sa, const primer_rec *o, int dir, const p3_global_settings *pa, const seq_lib *seqlib, int print_lib_sim) { const char *format1; char *seq = (FORWARD == dir) ? pr_oligo_sequence(sa, o) : pr_oligo_rev_c_sequence(sa, o); if(pa->thermodynamic_oligo_alignment==1) { format1 = "%-16s %5d %4d %7.2f %7.2f %5.2f %5.2f "; fprintf(f, format1, title, o->start + sa->incl_s + pa->first_base_index, o->length, o->temp, o->gc_content, o->self_any, o->self_end); fprintf(f, " %5.2f ", o->hairpin_th); } else { format1 = "%-16s %5d %4d %7.2f %7.2f %5.2f %5.2f "; fprintf(f, format1, title, o->start + sa->incl_s + pa->first_base_index, o->length, o->temp, o->gc_content, o->self_any, o->self_end); } if (print_lib_sim) { if (seqlib != NULL) fprintf(f, "%5.2f ", o->repeat_sim.score[o->repeat_sim.max]); else fprintf(f, "%5s ", ""); } fprintf(f, "%s\n", seq); if (PR_DEFAULT_INSIDE_PENALTY != pa->inside_penalty || PR_DEFAULT_OUTSIDE_PENALTY != pa->outside_penalty) fprintf(f, "POSITION PENALTY, QUALITY: %f, %f\n", o->position_penalty, o->quality); } static void print_pair_array(FILE *f, const char* title, int num, const interval_array_t array, const p3_global_settings *pa, const seq_args *sa) { int j; if (num > 0) { fprintf(f, "%s (start, len)*:", title); for (j = 0; j < num; j++) fprintf(f, " %d,%d", array[j][0] + pa->first_base_index + sa->incl_s, array[j][1]); fprintf(f, "\n"); } } static void print_2_pair_array(FILE *f, const char* title, int num, const interval_array_t left_array, const interval_array_t right_array, const p3_global_settings *pa, const seq_args *sa) { int j; if (num > 0) { fprintf(f, "%s (left_start, left_len, right_start, right_len)*:", title); for (j = 0; j < num; j++) { if ((left_array[j][0] == -1) && (left_array[j][1] == -1)) fprintf(f, " ,,"); else fprintf(f, " %d,%d,", left_array[j][0] + pa->first_base_index + sa->incl_s, left_array[j][1]); if ((right_array[j][0] == -1) && (right_array[j][1] == -1)) fprintf(f, ","); else fprintf(f, "%d,%d", right_array[j][0] + pa->first_base_index + sa->incl_s, right_array[j][1]); } fprintf(f, "\n"); } } #define VECTOR (1<<0) #define LEFT_OLIGO (1<<1) #define RIGHT_OLIGO (1<<2) #define INTL_OLIGO (1<<3) #define TARGET (1<<4) #define EXCL_REGION (1<<5) #define INTL_EXCL_REGION (1<<6) /* Prints out the asci picture of the sequence */ /* Return 1 on ENOMEM. Otherwise return 0. */ static int print_seq(FILE *f, const p3_global_settings *pa, const seq_args *sa, const p3retval *retval, primer_rec *h, const pair_array_t *best_pairs, int num) /* The number of primer pair to print. */ { primer_rec *h2 = NULL; primer_rec *h3 = NULL; int len, i, j, start; int something_found = 0, vector_found = 0; int *notes; char *notestr; primer_pair *p; p = NULL; if(retval->output_type == primer_pairs) { p = best_pairs->pairs + num; } len = strlen(sa->sequence); if (!(notes = (int*) malloc(sizeof(*notes) * len))) return 1; memset(notes, 0, sizeof(*notes) * len); if (!(notestr = (char*) malloc(len + 1))) { free(notes); return 1; } memset(notestr, ' ', len); notestr[len] = '\0'; for (i = 0; i < len; i++) { if (i < sa->incl_s || i >= sa->incl_s + sa->incl_l) notes[i] |= VECTOR; if (retval->output_type == primer_pairs && best_pairs->num_pairs > 0) { if (i >= p->left->start + sa->incl_s && i < p->left->start + p->left->length + sa->incl_s) notes[i] |= LEFT_OLIGO; if (i >= p->right->start - p->right->length + 1 + sa->incl_s && i <= p->right->start + sa->incl_s) notes[i] |= RIGHT_OLIGO; if ( pa->pick_internal_oligo == 1 && i >= p->intl->start + sa->incl_s && i < p->intl->start + p->intl->length + sa->incl_s) notes[i] |= INTL_OLIGO; } else if (h != NULL) { if(pa->pick_left_primer == 1 && i < h->start + h->length + sa->incl_s && i >= h->start + sa->incl_s) notes[i] |= LEFT_OLIGO; else if(pa->pick_right_primer == 1 && i >= h->start - h->length + 1 + sa->incl_s && i <= h->start + sa->incl_s) notes[i] |= RIGHT_OLIGO; else if(pa->pick_internal_oligo == 1 && i >= h->start + sa->incl_s && i < h->start + h->length + sa->incl_s) notes[i] |= INTL_OLIGO; } else if (pa->primer_task == pick_sequencing_primers) { if (pa->pick_right_primer && &retval->rev != NULL && retval->rev.num_elem > 0){ h2 = retval->rev.oligo; for (j = 0; j < pa->num_return; j++) { if(j > retval->rev.num_elem -1) break; h3 = h2 + j; if(i >= h3->start - h3->length + 1 + sa->incl_s && i <= h3->start + sa->incl_s) notes[i] |= RIGHT_OLIGO; } } if (pa->pick_left_primer && &retval->fwd != NULL && retval->fwd.num_elem > 0){ h2 = retval->fwd.oligo; for (j = 0; j < pa->num_return; j++) { if(j > retval->fwd.num_elem -1) break; h3 = h2 + j; if(i < h3->start + h3->length + sa->incl_s && i >= h3->start + sa->incl_s) { notes[i] |= LEFT_OLIGO; } } } } for (j = 0; j < sa->tar2.count; j++) { start = sa->tar2.pairs[j][0] + sa->incl_s; if (i >= start && i < start + sa->tar2.pairs[j][1]) notes[i] |= TARGET; } for (j = 0; j < sa->excl2.count; j++) { start = sa->excl2.pairs[j][0] + sa->incl_s; if (i >= start && i < start + sa->excl2.pairs[j][1]) notes[i] |= EXCL_REGION; } for (j = 0; j < sa->excl_internal2.count; j++) { start = sa->excl_internal2.pairs[j][0] + sa->incl_s; if (i >= start && i < start + sa->excl_internal2.pairs[j][1]) notes[i] |= INTL_EXCL_REGION; } } for (i = 0; i < len; i++) { if (notes[i] & VECTOR) { vector_found = 1; notestr[i] = '.'; } else if (notes[i] & EXCL_REGION) notestr[i] = 'X'; else if (notes[i] & INTL_EXCL_REGION) notestr[i] = 'x'; else if ((pa->primer_task == pick_sequencing_primers) && (notes[i] & LEFT_OLIGO) && (notes[i] & RIGHT_OLIGO)) notestr[i] = '^'; else if ((pa->primer_task == pick_sequencing_primers) && (notes[i] & LEFT_OLIGO)) notestr[i] = '>'; else if ((pa->primer_task == pick_sequencing_primers) && (notes[i] & RIGHT_OLIGO)) notestr[i] = '<'; else if ((notes[i] & TARGET) && (notes[i] & LEFT_OLIGO)) notestr[i] = ')'; else if ((notes[i] & TARGET) && (notes[i] & RIGHT_OLIGO)) notestr[i] = '('; else if (notes[i] & TARGET) notestr[i] = '*'; else if (notes[i] & LEFT_OLIGO) notestr[i] = '>'; else if (notes[i] & RIGHT_OLIGO) notestr[i] = '<'; else if (notes[i] & INTL_OLIGO) notestr[i] = '^'; if (notes[i] != 0) something_found = 1; } print_seq_lines(f, sa->sequence, notestr, len, 60, something_found, pa); if (something_found) fprintf(f, "KEYS (in order of precedence):\n"); if (vector_found) fprintf(f, "...... vector sequence\n"); if (sa->excl2.count > 0) fprintf(f, "XXXXXX excluded region\n"); if (pa->pick_internal_oligo ==1 && sa->excl_internal2.count > 0) fprintf(f, "xxxxxx excluded region for internal oligo\n"); if (sa->tar2.count > 0) fprintf(f, "****** target\n"); if (retval->output_type == primer_pairs && best_pairs->num_pairs > 0) { fprintf(f, ">>>>>> left primer\n"); fprintf(f, "<<<<<< right primer\n"); if ( pa->pick_internal_oligo == 1 ) fprintf(f, "^^^^^^ internal oligo\n"); } else if (pa->primer_task == pick_sequencing_primers) { fprintf(f, ">>>>>> left primer\n"); fprintf(f, "<<<<<< right primer\n"); fprintf(f, "^^^^^^ left primer / right primer overlap\n"); } else if (pa->pick_left_primer == 1 && h != NULL) fprintf(f, ">>>>>> left primer\n"); else if (pa->pick_right_primer == 1 && h != NULL) fprintf(f, "<<<<<< right primer\n"); else if (pa->pick_internal_oligo == 1 && h != NULL) fprintf(f, "^^^^^^ internal oligo\n"); if (something_found) fputc('\n', f); free(notes); free(notestr); return 0; } static void print_seq_lines(FILE *f, const char *s, const char *n, int seq_size, int line_size, int something_found, const p3_global_settings *pa) { int i = 0; while (seq_size > line_size) { fprintf(f, "%5d ", i + pa->first_base_index); fwrite(s, sizeof(*s), line_size, f); fputc('\n', f); if (something_found) { fprintf(f, " "); fwrite(n, sizeof(*n), line_size, f); fprintf(f, "\n\n"); } seq_size -= line_size; s += line_size; n += line_size; i += line_size; } if (something_found) fprintf(f, "%5d %s\n %s\n\n", i + pa->first_base_index, s, n); else fprintf(f, "%5d %s\n\n", i + pa->first_base_index, s); } static void print_pair_info(FILE *f, const primer_pair *p, const p3_global_settings *pa) { fprintf(f, "PRODUCT SIZE: %d, ", p->product_size); if(pa->thermodynamic_oligo_alignment==0) fprintf(f, "PAIR ANY COMPL: %.2f, PAIR 3' COMPL: %.2f\n", p->compl_any, p->compl_end); else fprintf(f, "PAIR ANY_TH COMPL: %.2f, PAIR 3'_TH COMPL: %.2f\n", p->compl_any, p->compl_end); if (pa->product_max_tm != PR_DEFAULT_PRODUCT_MAX_TM || pa->product_min_tm != PR_DEFAULT_PRODUCT_MIN_TM) { printf("PRODUCT Tm: %.4f, ", p->product_tm); printf("PRODUCT Tm - min(OLIGO Tm): %.4f\n", p->product_tm_oligo_tm_diff); } } static void print_rest(FILE *f, const p3_global_settings *pa, const seq_args *sa, const pair_array_t *best_pairs) { int i; int print_lib_sim = lib_sim_specified(pa); fprintf(f, "ADDITIONAL OLIGOS\n"); fprintf(f, " "); print_oligo_header(f, "", print_lib_sim, pa->thermodynamic_oligo_alignment); for (i = 1; i < best_pairs->num_pairs; i++) { fprintf(f, "\n%2d ", i); print_oligo(f, "LEFT PRIMER", sa, best_pairs->pairs[i].left, FORWARD, pa, pa->p_args.repeat_lib, print_lib_sim); fprintf(f, " "); print_oligo(f, "RIGHT PRIMER", sa, best_pairs->pairs[i].right, REVERSE, pa, pa->p_args.repeat_lib, print_lib_sim); if ( pa->pick_internal_oligo == 1) { fprintf(f, " "); print_oligo(f, "INTERNAL OLIGO", sa, best_pairs->pairs[i].intl, FORWARD, pa, pa->o_args.repeat_lib, print_lib_sim); } if (best_pairs->pairs[i].product_size > 0) { fprintf(f, " "); print_pair_info(f, &best_pairs->pairs[i], pa); } } } /* Print out the statistics of primer picking */ /* This function does _not_ print out the no_orf statistic. */ static void print_explain(FILE *f, const p3_global_settings *pa, const seq_args *sa, const p3retval *retval, int print_lib_sim, const char *pr_release) { const char *format; /* Format string for the table headers */ if(pa->thermodynamic_oligo_alignment==0) format = "%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s"; else format = "%6s%6s%6s%6s%6s%6s%6s%6s%6s%6s%7s%6s"; fprintf(f, "\nStatistics\n"); if (!pa->pick_anyway || !( (pa->pick_left_primer == 1 && pa->pick_internal_oligo == 0 && pa->pick_right_primer == 1 && sa->left_input && sa->right_input) || (pa->pick_left_primer == 1 && pa->pick_internal_oligo == 1 && pa->pick_right_primer == 1 && sa->left_input && sa->right_input && sa->internal_input) || (pa->pick_left_primer == 1 && pa->pick_internal_oligo == 0 && pa->pick_right_primer == 0 && sa->left_input) || (pa->pick_left_primer == 0 && pa->pick_internal_oligo == 0 && pa->pick_right_primer == 1 && sa->right_input) || (pa->pick_left_primer == 0 && pa->pick_internal_oligo == 1 && pa->pick_right_primer == 0 && sa->internal_input))) { /* print first row of header */ if(pa->thermodynamic_oligo_alignment==0) fprintf(f, format, "", "con", "too", "in", "in", "not", "", "no", "tm", "tm", " high", "high"); else fprintf(f, format, "", "con", "too", "in", "in", "not", "", "no", "tm", "tm", " high", "high"); if(pa->thermodynamic_oligo_alignment==1) fprintf(f, "%6s", "high"); if(print_lib_sim) fprintf(f, "%6s", "high"); fprintf(f, "%6s%6s", "", "high"); if(pa->lowercase_masking) fprintf(f, "%6s", "lower"); fprintf(f, "%6s\n", ""); /* print second row of header */ if(pa->thermodynamic_oligo_alignment==0) { fprintf(f, format, "", "sid", "many", "tar", "excl", "ok", "bad","GC", "too", "too", "any", "3'"); } else { fprintf(f, format, "", "sid", "many", "tar", "excl", "ok", "bad","GC", "too", "too", " any_th", "3'_th"); fprintf(f, "%6s", "hair-"); } if(print_lib_sim) fprintf(f, "%6s", "lib"); fprintf(f, "%6s%6s", "poly", "end"); if(pa->lowercase_masking) fprintf(f, "%6s", " case"); fprintf(f, "%6s\n", ""); /* print third row of header */ if(pa->thermodynamic_oligo_alignment==0) fprintf(f, format, "", "ered","Ns", "get", "reg", "reg", "GC%", "clamp", "low", "high"," compl", "compl"); else fprintf(f, format, "", "ered","Ns", "get", "reg", "reg", "GC%", "clamp", "low", "high"," compl", "compl"); if(pa->thermodynamic_oligo_alignment==1) fprintf(f, "%6s", " pin"); if(print_lib_sim) fprintf(f, "%6s", "sim"); fprintf(f, "%6s%6s", "X", "stab"); if(pa->lowercase_masking) fprintf(f, "%6s", " end"); if(pa->lowercase_masking) fprintf(f, "%6s\n", "ok "); else fprintf(f, "%6s\n", "ok"); } if (pa->pick_left_primer == 1 && !(pa->pick_anyway && sa->left_input)) print_stat_line(f, "Left", retval->fwd.expl, print_lib_sim, pa->lowercase_masking, pa->thermodynamic_oligo_alignment); if (pa->pick_right_primer == 1 && !(pa->pick_anyway && sa->right_input)) print_stat_line(f, "Right", retval->rev.expl, print_lib_sim, pa->lowercase_masking, pa->thermodynamic_oligo_alignment); if (pa->pick_internal_oligo == 1 && !(pa->pick_anyway && sa->internal_input)) print_stat_line(f, "Intl", retval->intl.expl, print_lib_sim, pa->lowercase_masking, pa->thermodynamic_oligo_alignment); if (pa->pick_left_primer == 1 && pa->pick_right_primer == 1) { fprintf(f, "Pair Stats:\n%s\n", p3_get_pair_array_explain_string(p3_get_rv_best_pairs(retval))); } fprintf(f, "%s\n", pr_release); } static void print_stat_line(FILE *f, const char *t, oligo_stats s, int print_lib_sim, int lowercase_masking, int thermodynamic_oligo_alignment) { const char *format = "%-6s%6d%6d%6d%6d%6d%6d%6d%6d%6d"; if (!strcmp(t, "Left")) fprintf(f,format, t, s.considered, s.ns, s.target, s.excluded, s.not_in_any_left_ok_region, s.gc, s.gc_clamp, s.temp_min, s.temp_max); else if (!strcmp(t, "Right")) fprintf(f,format, t, s.considered, s.ns, s.target, s.excluded, s.not_in_any_right_ok_region, s.gc, s.gc_clamp, s.temp_min, s.temp_max); else fprintf(f,format, t, s.considered, s.ns, s.target, s.excluded, 0, s.gc, s.gc_clamp, s.temp_min, s.temp_max); if(thermodynamic_oligo_alignment) { fprintf(f, " %6d%6d%6d", s.compl_any, s.compl_end, s.hairpin_th); } else { fprintf(f, "%6d%6d", s.compl_any, s.compl_end); } if (print_lib_sim) fprintf(f, "%6d",s.repeat_score); fprintf(f, "%6d%6d",s.poly_x, s.stability); if (lowercase_masking) fprintf(f, "%6d",s.gmasked); fprintf(f, "%6d\n", s.ok); } /* * Return true iff a check for library similarity has been specified for * either the primer pair or the internal oligo. */ static int lib_sim_specified(const p3_global_settings *pa) { return (pa->p_args.repeat_lib || pa->o_args.repeat_lib); } void format_error(FILE *f, const char* seq_name, const char *err) { if (NULL != seq_name) fprintf(f, "PRIMER PICKING RESULTS FOR %s\n\n", seq_name); if (err != NULL) fprintf(f, "INPUT PROBLEM: %s\n\n", err); } void format_warning(FILE *f, const char* seq_name, const char *err) { if (NULL != seq_name) fprintf(f, "WARNINGS FOR %s\n\n", seq_name); if (err != NULL) fprintf(f, "INPUT PROBLEM: %s\n\n", err); } /* Format and print out one oligo */ static void format_oligos(FILE *f, const p3_global_settings *pa, const seq_args *sa, const p3retval *retval, const char* pr_release, const pr_append_str *combined_retval_err, int explain_flag) { char *warning; int print_lib_sim = lib_sim_specified(pa); int i; int print_primers = 0; primer_rec *h = NULL; pair_array_t *best_pairs; primer_rec *p; int rest_count = 0; PR_ASSERT(NULL != f); PR_ASSERT(NULL != pa); PR_ASSERT(NULL != sa); best_pairs = NULL; if (!pr_is_empty(combined_retval_err)) { format_error(f, sa->sequence_name, pr_append_str_chars(combined_retval_err)); return; } if (NULL != sa->sequence_name) fprintf(f, "PRIMER PICKING RESULTS FOR %s\n\n", sa->sequence_name); if (pa->pick_left_primer || pa->pick_right_primer) { if (pa->p_args.repeat_lib != NULL) fprintf(f, "Using mispriming library %s\n", pa->p_args.repeat_lib->repeat_file); else fprintf(f, "No mispriming library specified\n"); } if (pa->pick_internal_oligo) { if (pa->o_args.repeat_lib != NULL) fprintf(f, "Using internal oligo mishyb library %s\n", pa->o_args.repeat_lib->repeat_file); else fprintf(f, "No internal oligo mishyb library specified\n"); } fprintf(f, "Using %d-based sequence positions\n", pa->first_base_index); if (pa->pick_left_primer) { if (retval->fwd.num_elem == 0){ fprintf(f, "NO LEFT PRIMER FOUND\n\n"); } else { print_primers = 1; } } if (pa->pick_internal_oligo) { if (retval->intl.num_elem == 0){ fprintf(f, "NO INTERNAL OLIGO FOUND\n\n"); } else { print_primers = 1; } } if (pa->pick_right_primer) { if (retval->rev.num_elem == 0){ fprintf(f, "NO RIGHT PRIMER FOUND\n\n"); } else { print_primers = 1; } } if ((warning = p3_get_rv_and_gs_warnings(retval, pa)) != NULL) { fprintf(f, "WARNING: %s\n\n", warning); free(warning); } if ((pa->primer_task != pick_primer_list ) && (pa->primer_task != pick_sequencing_primers)) { if (print_primers == 1) { print_oligo_header(f, "OLIGO", print_lib_sim, pa->thermodynamic_oligo_alignment); } /* Print out the first line with the best primers */ if ((pa->pick_left_primer) && (&retval->fwd != NULL ) && (retval->fwd.num_elem > 0)){ print_oligo(f, "LEFT_PRIMER", sa, retval->fwd.oligo, FORWARD, pa, pa->p_args.repeat_lib, print_lib_sim); h = retval->fwd.oligo; rest_count = 1; } if ((pa->pick_internal_oligo) && (&retval->intl != NULL ) && (retval->intl.num_elem > 0)){ print_oligo(f, "INTERNAL_OLIGO", sa, retval->intl.oligo, FORWARD, pa, pa->p_args.repeat_lib, print_lib_sim); h = retval->intl.oligo; rest_count = 1; } if ((pa->pick_right_primer) && (&retval->rev != NULL ) && (retval->rev.num_elem > 0)) { print_oligo(f, "RIGHT_PRIMER", sa, retval->rev.oligo, REVERSE, pa, pa->p_args.repeat_lib, print_lib_sim); h = retval->rev.oligo; rest_count = 1; } } if(print_primers == 1) { fprintf(f, "SEQUENCE SIZE: %ld\n", (long int) strlen(sa->sequence)); fprintf(f, "INCLUDED REGION SIZE: %d\n\n", sa->incl_l); print_pair_array(f, "TARGETS", sa->tar2.count, sa->tar2.pairs, pa, sa); print_pair_array(f, "EXCLUDED REGIONS", sa->excl2.count, sa->excl2.pairs, pa, sa); print_pair_array(f, "INTERNAL OLIGO EXCLUDED REGIONS", sa->excl_internal2.count, sa->excl_internal2.pairs, pa, sa); print_2_pair_array(f, "PAIR_OK_REGIONS", sa->ok_regions.count, sa->ok_regions.left_pairs, sa->ok_regions.right_pairs, pa, sa); } if (pa->primer_task != pick_primer_list ) { if (print_seq(f, pa, sa, retval, h, best_pairs, 0)) exit(-2); /* ENOMEM */ } fprintf(f, "\n"); /* Print out the other primers */ if ((pa->pick_left_primer) && (&retval->fwd != NULL ) && (retval->fwd.num_elem > rest_count)){ int n = retval->fwd.num_elem; h = retval->fwd.oligo; if (rest_count == 1) { fprintf(f, "ADDITIONAL OLIGOS\n"); } fprintf(f, " "); print_oligo_header(f, "", print_lib_sim, pa->thermodynamic_oligo_alignment); for (i = rest_count; i < pa->num_return; i++) { if(i > n-1) break; p = h + i; fprintf(f, "%2d ", i + 1 - rest_count); print_oligo(f, "LEFT_PRIMER", sa, p, FORWARD, pa, pa->p_args.repeat_lib, print_lib_sim); } if (rest_count == 0) { fprintf(f, "\n "); } } if ((pa->pick_internal_oligo) && (&retval->intl != NULL ) && (retval->intl.num_elem > rest_count)){ int n = retval->intl.num_elem; h = retval->intl.oligo; if (rest_count == 1) { fprintf(f, "ADDITIONAL OLIGOS\n"); } fprintf(f, " "); print_oligo_header(f, "", print_lib_sim, pa->thermodynamic_oligo_alignment); for (i = rest_count; i < pa->num_return; i++) { if(i > n-1) break; p = h + i; fprintf(f, "%2d ", i + 1 - rest_count); print_oligo(f, "INTERNAL_OLIGO", sa, p, FORWARD, pa, pa->p_args.repeat_lib, print_lib_sim); } if (rest_count == 0) { fprintf(f, "\n "); } } if ((pa->pick_right_primer) && (&retval->rev != NULL ) && (retval->rev.num_elem > rest_count)) { int n = retval->rev.num_elem; h = retval->rev.oligo; if (rest_count == 1) { fprintf(f, "ADDITIONAL OLIGOS\n"); } fprintf(f, " "); print_oligo_header(f, "", print_lib_sim, pa->thermodynamic_oligo_alignment); for (i = rest_count; i < pa->num_return; i++) { if(i > n-1) break; p = h + i; fprintf(f, "%2d ", i + 1 - rest_count); print_oligo(f, "RIGHT_PRIMER", sa, p, REVERSE, pa, pa->p_args.repeat_lib, print_lib_sim); } } if (explain_flag) print_explain(f, pa, sa, retval, print_lib_sim, pr_release); fprintf(f, "\n\n"); if (fflush(f) == EOF) { perror("fflush(f) failed"); exit(-1); } } primer3-2.3.6/src/thal.c0000775000077300007730000025716612231156245013765 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2009,2010, 2011,2012 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), and Helen Skaletsky All rights reserved. This file is part of primer3 software suite. This software suite is is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this software (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON A THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include #include #include #include #include #include #include #include #include #if defined(__sun) #include #endif #include "thal.h" /*#define DEBUG*/ #ifndef MIN_HRPN_LOOP #define MIN_HRPN_LOOP 3 /* minimum size of hairpin loop */ #endif #ifndef THAL_EXIT_ON_ERROR #define THAL_EXIT_ON_ERROR 0 #endif /* table where bp-s enthalpies, that retrieve to the most stable Tm, are saved */ #ifdef EnthalpyDPT # undef EnthalpyDPT #endif #define EnthalpyDPT(i, j) enthalpyDPT[(j) + ((i-1)*len3) - (1)] /* table where bp-s entropies, that retrieve to the most stable Tm, are saved */ #ifdef EntropyDPT # undef EntropyDPT #endif #define EntropyDPT(i, j) entropyDPT[(j) + ((i-1)*len3) - (1)] /* entropies of most stable hairpin terminal bp */ #ifndef SEND5 # define SEND5(i) send5[i] #endif /* enthalpies of most stable hairpin terminal bp */ #ifndef HEND5 # define HEND5(i) hend5[i] #endif #define CHECK_ERROR(COND,MSG) if (COND) { strcpy(o->msg, MSG); errno = 0; longjmp(_jmp_buf, 1); } #define THAL_OOM_ERROR { strcpy(o->msg, "Out of memory"); errno = ENOMEM; longjmp(_jmp_buf, 1); } #define THAL_IO_ERROR(f) { sprintf(o->msg, "Unable to open file %s", f); longjmp(_jmp_buf, 1); } #define bpIndx(a, b) BPI[a][b] /* for traceing matrix BPI */ #define atPenaltyS(a, b) atpS[a][b] #define atPenaltyH(a, b) atpH[a][b] #define STR(X) #X #define LONG_SEQ_ERR_STR(MAX_LEN) "Target sequence length > maximum allowed (" STR(MAX_LEN) ") in thermodynamic alignment" #define XSTR(X) STR(X) #define SMALL_NON_ZERO 0.000001 #define DBL_EQ(X,Y) (((X) - (Y)) < (SMALL_NON_ZERO) ? (1) : (2)) /* 1 when numbers are equal */ #ifdef INTEGER # define isFinite(x) (x < _INFINITY / 2) #else # define isFinite(x) finite(x) #endif #define isPositive(x) ((x) > 0 ? (1) : (0)) /*** BEGIN CONSTANTS ***/ # ifdef INTEGER const double _INFINITY = 999999.0; # else # ifdef INFINITY const double _INFINITY = INFINITY; # else const double _INFINITY = 1.0 / 0.0; # endif # endif static const double R = 1.9872; /* cal/Kmol */ static const double ILAS = (-300 / 310.15); /* Internal Loop Entropy ASymmetry correction -0.3kcal/mol*/ static const double ILAH = 0.0; /* Internal Loop EntHalpy Asymmetry correction */ static const double AT_H = 2200.0; /* AT penalty */ static const double AT_S = 6.9; /* AT penalty */ static const double MinEntropyCutoff = -2500.0; /* to filter out non-existing entropies */ static const double MinEntropy = -3224.0; /* initiation */ static const double G2 = 0.0; /* structures w higher G are considered to be unstabile */ const double ABSOLUTE_ZERO = 273.15; const int MAX_LOOP = 30; /* the maximum size of loop that can be calculated; for larger loops formula must be implemented */ const int MIN_LOOP = 0; static const char BASES[5] = {'A', 'C', 'G', 'T', 'N'}; /* bases to be considered - N is every symbol that is not A, G, C,$ */ static const char BASE_PAIRS[4][4] = {"A-T", "C-G", "G-C", "T-A" }; /* allowed basepairs */ /* matrix for allowed; bp 0 - no bp, watson crick bp - 1 */ static const int BPI[5][5] = { {0, 0, 0, 1, 0}, /* A, C, G, T, N; */ {0, 0, 1, 0, 0}, {0, 1, 0, 0, 0}, {1, 0, 0, 0, 0}, {0, 0, 0, 0, 0}}; /*** END OF CONSTANTS ***/ /*** BEGIN STRUCTs ***/ struct triloop { char loop[5]; double value; }; struct tetraloop { char loop[6]; double value; }; struct tracer /* structure for tracebacku - unimolecular str */ { int i; int j; int mtrx; /* [0 1] EntropyDPT/EnthalpyDPT*/ struct tracer* next; }; /*** END STRUCTs ***/ static int length_unsig_char(const unsigned char * str); /* returns length of unsigned char; to avoid warnings while compiling */ static unsigned char str2int(char c); /* converts DNA sequence to int; 0-A, 1-C, 2-G, 3-T, 4-whatever */ static double saltCorrectS (double mv, double dv, double dntp); /* part of calculating salt correction for Tm by SantaLucia et al */ static FILE* openParamFile(const char* name, thal_results* o); /* file of thermodynamic params */ /* get thermodynamic tables */ static double readDouble(FILE *file, thal_results* o); static void readLoop(FILE *file, double *v1, double *v2, double *v3, thal_results *o); static int readTLoop(FILE *file, char *s, double *v, int triloop, thal_results *o); static void getStack(double stackEntropies[5][5][5][5], double stackEnthalpies[5][5][5][5], thal_results* o); /*static void verifyStackTable(double stack[5][5][5][5], char* type);*/ /* just for debugging; the method is turned off by default */ static void getStackint2(double stackEntropiesint2[5][5][5][5], double stackint2Enthalpies[5][5][5][5], thal_results* o); static void getDangle(double dangleEntropies3[5][5][5], double dangleEnthalpies3[5][5][5], double dangleEntropies5[5][5][5], double dangleEnthalpies5[5][5][5], thal_results* o); static void getTstack(double tstackEntropies[5][5][5][5], double tstackEnthalpies[5][5][5][5], thal_results* o); static void getTstack2(double tstack2Entropies[5][5][5][5], double tstack2Enthalpies[5][5][5][5], thal_results* o); static void getTriloop(struct triloop**, struct triloop**, int* num, thal_results* o); static void getTetraloop(struct tetraloop**, struct tetraloop**, int* num, thal_results* o); static void getLoop(double hairpinLoopEnntropies[30], double interiorLoopEntropies[30], double bulgeLoopEntropiess[30], double hairpinLoopEnthalpies[30], double interiorLoopEnthalpies[30], double bulgeLoopEnthalpies[30], thal_results* o); static void tableStartATS(double atp_value, double atp[5][5]); /* creates table of entropy values for nucleotides to which AT-penlty must be applied */ static void tableStartATH(double atp_value, double atp[5][5]); static int comp3loop(const void*, const void*); /* checks if sequnece consists of specific triloop */ static int comp4loop(const void*, const void*); /* checks if sequnece consists of specific tetraloop */ static void initMatrix(); /* initiates thermodynamic parameter tables of entropy and enthalpy for dimer */ static void initMatrix2(); /* initiates thermodynamic parameter tables of entropy and enthalpy for monomer */ static void fillMatrix(int maxLoop, thal_results* o); /* calc-s thermod values into dynamic progr table (dimer) */ static void fillMatrix2(int maxLoop, thal_results* o); /* calc-s thermod values into dynamic progr table (monomer) */ static void maxTM(int i, int j); /* finds max Tm while filling the dyn progr table using stacking S and stacking H (dimer) */ static void maxTM2(int i, int j); /* finds max Tm while filling the dyn progr table using stacking S and stacking H (monomer) */ /* calculates bulges and internal loops for dimer structures */ static void calc_bulge_internal(int ii, int jj, int i, int j, double* EntropyEnthalpy, int traceback, int maxLoop); /* calculates bulges and internal loops for monomer structures */ static void calc_bulge_internal2(int ii, int jj, int i, int j, double* EntropyEnthalpy, int traceback, int maxLoop); /* carries out Bulge and Internal loop and stack calculations to hairpin */ static void CBI(int i, int j, double* EntropyEnthalpy, int traceback, int maxLoop); /* finds monomer structure that has maximum Tm */ static void calc_hairpin(int i, int j, double* EntropyEnthalpy, int traceback); static double Ss(int i, int j, int k); /* returns stack entropy */ static double Hs(int i, int j, int k); /* returns stack enthalpy */ /* calculate terminal entropy S and terminal enthalpy H starting reading from 5'end (Left hand/3' end - Right end) */ static void LSH(int i, int j, double* EntropyEnthalpy); static void RSH(int i, int j, double* EntropyEnthalpy); static void reverse(unsigned char *s); static int max5(double, double, double, double, double); /* Is sequence symmetrical */ static int symmetry_thermo(const unsigned char* seq); /* traceback for dimers */ static void traceback(int i, int j, double RT, int* ps1, int* ps2, int maxLoop, thal_results* o); /* traceback for hairpins */ static void tracebacku(int*, int, thal_results*); /* prints ascii output of dimer structure */ static void drawDimer(int*, int*, double, double, double, int, double, thal_results *); /* prints ascii output of hairpin structure */ static void drawHairpin(int*, double, double, int, double, thal_results *); static int equal(double a, double b); static void strcatc(char*, char); static void push(struct tracer**, int, int, int, thal_results*); /* to add elements to struct */ /* terminal bp for monomer structure */ static void calc_terminal_bp(double temp); /* executed in calc_terminal_bp; to find structure that corresponds to max Tm for terminal bp */ static double END5_1(int,int); /* END5_1(X,1/2) - 1=Enthalpy, 2=Entropy*/ static double END5_2(int,int); static double END5_3(int,int); static double END5_4(int,int); static double Hd5(int,int); /* returns thermodynamic value (H) for 5' dangling end */ static double Hd3(int,int); /* returns thermodynamic value (H) for 3' dangling end */ static double Sd5(int,int); /* returns thermodynamic value (S) for 5' dangling end */ static double Sd3(int,int); /* returns thermodynamic value (S) for 3' dangling end */ static double Ststack(int,int); /* returns entropy value for terminal stack */ static double Htstack(int,int); /* returns enthalpy value for terminal stack */ /* memory stuff */ static void* safe_calloc(size_t, size_t, thal_results* o); static void* safe_malloc(size_t, thal_results* o); static void* safe_realloc(void*, size_t, thal_results* o); static double* safe_recalloc(double* ptr, int m, int n, thal_results* o); static char* parampath = NULL; /* path to parameter files */ static int numTriloops; /* hairpin triloop penalties */ static int numTetraloops; /* hairpin tetraloop penalties */ static double atpS[5][5]; /* AT penalty */ static double atpH[5][5]; /* AT penalty */ static double *send5, *hend5; /* calc 5' */ /* w/o init not constant anymore, cause for unimolecular and bimolecular foldings there are different values */ static double dplx_init_H; /* initiation enthalpy; for duplex 200, for unimolecular structure 0 */ static double dplx_init_S; /* initiation entropy; for duplex -5.7, for unimoleculat structure 0 */ static double saltCorrection; /* value calculated by saltCorrectS, includes correction for monovalent and divalent cations */ static double RC; /* universal gas constant multiplied w DNA conc - for melting temperature */ static double SHleft; /* var that helps to find str w highest melting temperature */ static int bestI, bestJ; /* starting position of most stable str */ static double* enthalpyDPT; /* matrix for values of enthalpy */ static double* entropyDPT; /* matrix for values of entropy */ static unsigned char *oligo1, *oligo2; /* inserted oligo sequenced */ static unsigned char *numSeq1, *numSeq2; /* same as oligo1 and oligo2 but converted to numbers */ static int len1, len2, len3; /* length of sequense 1 and 2 *//* 17.02.2009 int temponly;*/ /* print only temperature of the predicted structure */ static double dangleEntropies3[5][5][5]; /* thermodynamic paramteres for 3' dangling ends */ static double dangleEnthalpies3[5][5][5]; /* ther params for 3' dangling ends */ static double dangleEntropies5[5][5][5]; /* ther params for 5' dangling ends */ static double dangleEnthalpies5[5][5][5]; /* ther params for 5' dangling ends */ static double stackEntropies[5][5][5][5]; /* ther params for perfect match pairs */ static double stackEnthalpies[5][5][5][5]; /* ther params for perfect match pairs */ static double stackint2Entropies[5][5][5][5]; /*ther params for perfect match and internal mm */ static double stackint2Enthalpies[5][5][5][5]; /* ther params for perfect match and internal mm*/ static double interiorLoopEntropies[30]; /* interior loop params according to length of the loop */ static double bulgeLoopEntropies[30]; /* bulge loop params according to length of the loop */ static double hairpinLoopEntropies[30]; /* hairpin loop params accordint to length of the loop */ static double interiorLoopEnthalpies[30]; /* same as interiorLoopEntropies but values of entropy */ static double bulgeLoopEnthalpies[30]; /* same as bulgeLoopEntropies but values of entropy */ static double hairpinLoopEnthalpies[30]; /* same as hairpinLoopEntropies but values of entropy */ static double tstackEntropies[5][5][5][5]; /* ther params for terminal mismatches */ static double tstackEnthalpies[5][5][5][5]; /* ther params for terminal mismatches */ static double tstack2Entropies[5][5][5][5]; /* ther params for internal terminal mismatches */ static double tstack2Enthalpies[5][5][5][5]; /* ther params for internal terminal mismatches */ static struct triloop* triloopEntropies = NULL; /* ther penalties for given triloop seq-s */ static struct triloop* triloopEnthalpies = NULL; /* ther penalties for given triloop seq-s */ static struct tetraloop* tetraloopEntropies = NULL; /* ther penalties for given tetraloop seq-s */ static struct tetraloop* tetraloopEnthalpies = NULL; /* ther penalties for given tetraloop seq-s */ static jmp_buf _jmp_buf; /* Read the thermodynamic values (parameters) from the parameter files in the directory specified by 'path'. Return 0 on success and -1 on error. The thermodynamic values are stored in multiple static variables. */ int get_thermodynamic_values(const char* path, thal_results *o) { if (setjmp(_jmp_buf) != 0) { return -1; } parampath = (char*) safe_malloc((strlen(path) + 1) * sizeof(char), o); strcpy(parampath, path); getStack(stackEntropies, stackEnthalpies, o); /* verifyStackTable(stackEntropies, "entropy"); verifyStackTable(stackEnthalpies, "enthalpy"); */ /* this is for code debugging */ getStackint2(stackint2Entropies, stackint2Enthalpies, o); getDangle(dangleEntropies3, dangleEnthalpies3, dangleEntropies5, dangleEnthalpies5, o); getLoop(hairpinLoopEntropies, interiorLoopEntropies, bulgeLoopEntropies, hairpinLoopEnthalpies, interiorLoopEnthalpies, bulgeLoopEnthalpies, o); getTstack(tstackEntropies, tstackEnthalpies, o); getTstack2(tstack2Entropies, tstack2Enthalpies, o); getTriloop(&triloopEntropies, &triloopEnthalpies, &numTriloops, o); getTetraloop(&tetraloopEntropies, &tetraloopEnthalpies, &numTetraloops, o); /* getting the AT-penalties */ tableStartATS(AT_S, atpS); tableStartATH(AT_H, atpH); return 0; } void destroy_thal_structures() { free(parampath); free(triloopEntropies); free(triloopEnthalpies); free(tetraloopEntropies); free(tetraloopEnthalpies); } /* central method: execute all sub-methods for calculating secondary structure for dimer or for monomer */ void thal(const unsigned char *oligo_f, const unsigned char *oligo_r, const thal_args *a, thal_results *o) { double* SH; int i, j; int len_f, len_r; double T1; int k; int *bp; unsigned char *oligo2_rev = NULL; double mh, ms; send5 = hend5 = NULL; enthalpyDPT = entropyDPT = NULL; numSeq1 = numSeq2 = NULL; oligo1 = oligo2 = NULL; strcpy(o->msg, ""); o->temp = THAL_ERROR_SCORE; errno = 0; if (setjmp(_jmp_buf) != 0) { o->temp = THAL_ERROR_SCORE; return; /* If we get here, that means we returned via a longjmp. In this case errno might be ENOMEM, but not necessarily. */ } CHECK_ERROR(NULL == oligo_f, "NULL first sequence"); CHECK_ERROR(NULL == oligo_r, "NULL second sequence"); len_f = length_unsig_char(oligo_f); len_r = length_unsig_char(oligo_r); /* The following error messages will be seen by end users and will not be easy to understand. */ CHECK_ERROR((len_f > THAL_MAX_ALIGN) && (len_r > THAL_MAX_ALIGN), "Both sequences longer than " XSTR(THAL_MAX_ALIGN) " for thermodynamic alignment"); CHECK_ERROR((len_f > THAL_MAX_SEQ), LONG_SEQ_ERR_STR(THAL_MAX_SEQ) " (1)"); CHECK_ERROR((len_r > THAL_MAX_SEQ), LONG_SEQ_ERR_STR(THAL_MAX_SEQ) " (2)"); CHECK_ERROR(NULL == a, "NULL 'in' pointer"); if (NULL == o) return; /* Leave it to the caller to crash */ CHECK_ERROR(a->type != thal_any && a->type != thal_end1 && a->type != thal_end2 && a->type != thal_hairpin, "Illegal type"); o->align_end_1 = -1; o->align_end_2 = -1; if ('\0' == oligo_f) { strcpy(o->msg, "Empty first sequence"); o->temp = 0.0; return; } if ('\0' == oligo_r) { strcpy(o->msg, "Empty second sequence"); o->temp = 0.0; return; } if (0 == len_f) { o->temp = 0.0; return; } if (0 == len_r) { o->temp = 0.0; return; } if(a->type!=3) { oligo1 = (unsigned char*) safe_malloc((len_f + 1) * sizeof(unsigned char), o); oligo2 = (unsigned char*) safe_malloc((len_r + 1) * sizeof(unsigned char), o); strcpy((char*)oligo1,(const char*)oligo_f); strcpy((char*)oligo2,(const char*)oligo_r); } else { oligo1 = (unsigned char*) safe_malloc((len_r + 1) * sizeof(unsigned char), o); oligo2 = (unsigned char*) safe_malloc((len_f + 1) * sizeof(unsigned char), o); strcpy((char*)oligo1,(const char*)oligo_r); strcpy((char*)oligo2,(const char*)oligo_f); } /*** INIT values for unimolecular and bimolecular structures ***/ if (a->type==4) { /* unimolecular folding */ len2 = length_unsig_char(oligo2); len3 = len2 -1; dplx_init_H = 0.0; dplx_init_S = -0.00000000001; RC=0; } else if(a->type!=4) { /* hybridization of two oligos */ dplx_init_H = 200; dplx_init_S = -5.7; if(symmetry_thermo(oligo1) && symmetry_thermo(oligo2)) { RC = R * log(a->dna_conc/1000000000.0); } else { RC = R * log(a->dna_conc/4000000000.0); } if(a->type!=3) { oligo2_rev = (unsigned char*) safe_malloc((length_unsig_char(oligo_r) + 1) * sizeof(unsigned char), o); strcpy((char*)oligo2_rev,(const char*)oligo_r); } else { oligo2_rev = (unsigned char*) safe_malloc((length_unsig_char(oligo_f) + 1) * sizeof(unsigned char), o); strcpy((char*)oligo2_rev,(const char*)oligo_f); } reverse(oligo2_rev); /* REVERSE oligo2, so it goes to dpt 3'->5' direction */ free(oligo2); oligo2=NULL; oligo2=&oligo2_rev[0]; } else { strcpy(o->msg, "Wrong alignment type!"); o->temp = THAL_ERROR_SCORE; errno=0; #ifdef DEBUG fprintf(stderr, o->msg); #endif return; } len1 = length_unsig_char(oligo1); len2 = length_unsig_char(oligo2); /* convert nucleotides to numbers */ numSeq1 = (unsigned char*) safe_realloc(numSeq1, len1 + 2, o); numSeq2 = (unsigned char*) safe_realloc(numSeq2, len2 + 2, o); /*** Calc part of the salt correction ***/ saltCorrection=saltCorrectS(a->mv,a->dv,a->dntp); /* salt correction for entropy, must be multiplied with N, which is the total number of phosphates in the duplex divided by 2; 8bp dplx N=7 */ if(a->type == 4){ /* monomer */ /* terminal basepairs */ send5 = (double*) safe_realloc(send5, (len1 + 1) * sizeof(double), o); hend5 = (double*) safe_realloc(hend5, (len1 + 1) * sizeof(double), o); } for(i = 0; i < len1; i++) oligo1[i] = toupper(oligo1[i]); for(i = 0; i < len2; i++) oligo2[i] = toupper(oligo2[i]); for(i = 1; i <= len1; ++i) numSeq1[i] = str2int(oligo1[i - 1]); for(i = 1; i <= len2; ++i) numSeq2[i] = str2int(oligo2[i - 1]); numSeq1[0] = numSeq1[len1 + 1] = numSeq2[0] = numSeq2[len2 + 1] = 4; /* mark as N-s */ if (a->type==4) { /* calculate structure of monomer */ enthalpyDPT = safe_recalloc(enthalpyDPT, len1, len2, o); entropyDPT = safe_recalloc(entropyDPT, len1, len2, o); initMatrix2(); fillMatrix2(a->maxLoop, o); calc_terminal_bp(a->temp); mh = HEND5(len1); ms = SEND5(len1); o->align_end_1 = (int) mh; o->align_end_2 = (int) ms; bp = (int*) safe_calloc(len1, sizeof(int), o); for (k = 0; k < len1; ++k) bp[k] = 0; if(isFinite(mh)) { tracebacku(bp, a->maxLoop, o); /* traceback for unimolecular structure */ drawHairpin(bp, mh, ms, a->temponly,a->temp, o); /* if temponly=1 then return after printing basic therm data */ } else if(a->temponly==0) { fputs("No secondary structure could be calculated\n",stderr); } if(o->temp==-_INFINITY && (!strcmp(o->msg, ""))) o->temp=0.0; free(bp); free(enthalpyDPT); free(entropyDPT); free(numSeq1); free(numSeq2); free(send5); free(hend5); free(oligo1); free(oligo2); return; } else if(a->type!=4) { /* Hybridization of two moleculs */ len3 = len2; enthalpyDPT = safe_recalloc(enthalpyDPT, len1, len2, o); /* dyn. programming table for dS and dH */ entropyDPT = safe_recalloc(entropyDPT, len1, len2, o); /* enthalpyDPT is 3D array represented as 1D array */ initMatrix(); fillMatrix(a->maxLoop, o); SHleft = -_INFINITY; SH = (double*) safe_malloc(2 * sizeof(double), o); /* calculate terminal basepairs */ bestI = bestJ = 0; if(a->type==1) for (i = 1; i <= len1; i++) { for (j = 1; j <= len2; j++) { RSH(i, j, SH); SH[0] = SH[0]+SMALL_NON_ZERO; /* this adding is done for compiler, optimization -O2 vs -O0 */ SH[1] = SH[1]+SMALL_NON_ZERO; T1 = ((EnthalpyDPT(i, j)+ SH[1] + dplx_init_H) / ((EntropyDPT(i, j)) + SH[0] + dplx_init_S + RC)) - ABSOLUTE_ZERO; if (T1 > SHleft && ((EntropyDPT(i, j) + SH[0])<0 && (SH[1] + EnthalpyDPT(i, j))<0)) { SHleft = T1; bestI = i; bestJ = j; } } } int *ps1, *ps2; ps1 = (int*) safe_calloc(len1, sizeof(int), o); ps2 = (int*) safe_calloc(len2, sizeof(int), o); for (i = 0; i < len1; ++i) ps1[i] = 0; for (j = 0; j < len2; ++j) ps2[j] = 0; if(a->type == 2 || a->type == 3) { /* THAL_END1 */ bestI = bestJ = 0; bestI = len1; i = len1; SHleft = -_INFINITY; for (j = 1; j <= len2; ++j) { RSH(i, j, SH); SH[0] = SH[0]+SMALL_NON_ZERO; /* this adding is done for compiler, optimization -O2 vs -O0, that compiler could understand that SH is changed in this cycle */ SH[1] = SH[1]+SMALL_NON_ZERO; T1 = ((EnthalpyDPT(i, j)+ SH[1] + dplx_init_H) / ((EntropyDPT(i, j)) + SH[0] + dplx_init_S + RC)) - ABSOLUTE_ZERO; if (T1 > SHleft && ((SH[0] + EntropyDPT(i, j))<0 && (SH[1] + EnthalpyDPT(i, j))<0)) { SHleft = T1; bestJ = j; } } } if (!isFinite(SHleft)) bestI = bestJ = 1; double dH, dS; RSH(bestI, bestJ, SH); dH = EnthalpyDPT(bestI, bestJ)+ SH[1] + dplx_init_H; dS = (EntropyDPT(bestI, bestJ) + SH[0] + dplx_init_S); /* tracebacking */ for (i = 0; i < len1; ++i) ps1[i] = 0; for (j = 0; j < len2; ++j) ps2[j] = 0; if(isFinite(EnthalpyDPT(bestI, bestJ))){ traceback(bestI, bestJ, RC, ps1, ps2, a->maxLoop, o); drawDimer(ps1, ps2, SHleft, dH, dS, a->temponly,a->temp, o); o->align_end_1=bestI; o->align_end_2=bestJ; } else { o->temp = 0.0; } free(ps1); free(ps2); free(SH); free(oligo2_rev); free(enthalpyDPT); free(entropyDPT); free(numSeq1); free(numSeq2); free(oligo1); return; } return; } /*** END thal() ***/ /* Set default args */ void set_thal_default_args(thal_args *a) { memset(a, 0, sizeof(*a)); a->debug = 0; a->type = thal_any; /* thal_alignment_type THAL_ANY */ a->maxLoop = MAX_LOOP; a->mv = 50; /* mM */ a->dv = 0.0; /* mM */ a->dntp = 0.8; /* mM */ a->dna_conc = 50; /* nM */ a->temp = 310.15; /* Kelvin */ a->temponly = 1; /* return only melting temperature of predicted structure */ a->dimer = 1; /* by default dimer structure is calculated */ } /* Set default args for oligo */ void set_thal_oligo_default_args(thal_args *a) { memset(a, 0, sizeof(*a)); a->debug = 0; a->type = thal_any; /* thal_alignment_type THAL_ANY */ a->maxLoop = MAX_LOOP; a->mv = 50; /* mM */ a->dv = 0.0; /* mM */ a->dntp = 0.0; /* mM */ a->dna_conc = 50; /* nM */ a->temp = 310.15; /* Kelvin */ a->temponly = 1; /* return only melting temperature of predicted structure */ a->dimer = 1; /* by default dimer structure is calculated */ } static unsigned char str2int(char c) { switch (c) { case 'A': case '0': return 0; case 'C': case '1': return 1; case 'G': case '2': return 2; case 'T': case '3': return 3; } return 4; } /* memory stuff */ static double* safe_recalloc(double* ptr, int m, int n, thal_results* o) { return (double*) safe_realloc(ptr, m * n * sizeof(double), o); } static void* safe_calloc(size_t m, size_t n, thal_results *o) { void* ptr; if (!(ptr = calloc(m, n))) { #ifdef DEBUG fputs("Error in calloc()\n", stderr); #endif THAL_OOM_ERROR; } return ptr; } static void* safe_malloc(size_t n, thal_results *o) { void* ptr; if (!(ptr = malloc(n))) { #ifdef DEBUG fputs("Error in malloc()\n", stderr); #endif THAL_OOM_ERROR; } return ptr; } static void* safe_realloc(void* ptr, size_t n, thal_results *o) { ptr = realloc(ptr, n); if (ptr == NULL) { #ifdef DEBUG fputs("Error in realloc()\n", stderr); #endif THAL_OOM_ERROR; } return ptr; } static int max5(double a, double b, double c, double d, double e) { if(a > b && a > c && a > d && a > e) return 1; else if(b > c && b > d && b > e) return 2; else if(c > d && c > e) return 3; else if(d > e) return 4; else return 5; } static void push(struct tracer** stack, int i, int j, int mtrx, thal_results* o) { struct tracer* new_top; new_top = (struct tracer*) safe_malloc(sizeof(struct tracer), o); new_top->i = i; new_top->j = j; new_top->mtrx = mtrx; new_top->next = *stack; *stack = new_top; } static void reverse(unsigned char *s) { int i,j; char c; for (i = 0, j = length_unsig_char(s)-1; i < j; i++, j--) { c = s[i]; s[i] = s[j]; s[j] = c; } } static FILE* openParamFile(const char* fname, thal_results* o) { FILE* file; char* paramdir; file = fopen(fname, "rt"); if (!file) { paramdir = (char*) safe_malloc(strlen(parampath) + strlen(fname) + 1, o); strcpy(paramdir, parampath); strcat(paramdir, fname); if (!(file = fopen(paramdir, "rt"))) { #ifdef DEBUG perror(paramdir); #endif THAL_IO_ERROR(paramdir); free(paramdir); } free(paramdir); } return file; } static double saltCorrectS (double mv, double dv, double dntp) { if(dv<=0) dntp=dv; return 0.368*((log((mv+120*(sqrt(fmax(0.0, dv-dntp))))/1000))); } #define INIT_BUF_SIZE 1024 static char* p3_read_line(FILE *file, thal_results* o) { static size_t ssz; static char *s = NULL; size_t remaining_size; char *p, *n; if (NULL == s) { ssz = INIT_BUF_SIZE; s = (char *) safe_malloc(ssz, o); } p = s; remaining_size = ssz; while (1) { if (fgets(p, remaining_size, file) == NULL) /* End of file. */ return p == s ? NULL : s; if ((n = strchr(p, '\n')) != NULL) { *n = '\0'; return s; } /* We did not get the whole line. */ if (ssz >= INT_MAX / 2) ssz = INT_MAX; else { ssz *= 2; } s = (char *) safe_realloc(s, ssz, o); p = strchr(s, '\0'); remaining_size = ssz - (p - s); } } /* These functions are needed as "inf" cannot be read on Windows directly */ static double readDouble(FILE *file, thal_results* o) { double result; char *line = p3_read_line(file, o); /* skip any spaces at beginning of the line */ while (isspace(*line)) line++; if (!strncmp(line, "inf", 3)) return _INFINITY; sscanf(line, "%lf", &result); return result; } /* Reads a line containing 4 doubles, which can be specified as "inf". */ static void readLoop(FILE *file, double *v1, double *v2, double *v3, thal_results *o) { char *line = p3_read_line(file, o); char *p = line, *q; /* skip first number on the line */ while (isspace(*p)) p++; while (isdigit(*p)) p++; while (isspace(*p)) p++; /* read second number */ q = p; while (!isspace(*q)) q++; *q = '\0'; q++; if (!strcmp(p, "inf")) *v1 = _INFINITY; else sscanf(p, "%lf", v1); while (isspace(*q)) q++; /* read third number */ p = q; while (!isspace(*p)) p++; *p = '\0'; p++; if (!strcmp(q, "inf")) *v2 = _INFINITY; else sscanf(q, "%lf", v2); while (isspace(*p)) p++; /* read last number */ q = p; while (!isspace(*q) && (*q != '\0')) q++; *q = '\0'; if (!strcmp(p, "inf")) *v3 = _INFINITY; else sscanf(p, "%lf", v3); } /* Reads a line containing a short string and a double, used for reading a triloop or tetraloop. */ static int readTLoop(FILE *file, char *s, double *v, int triloop, thal_results *o) { char *line = p3_read_line(file, o); if (!line) return -1; char *p = line, *q; /* skip first spaces */ while (isspace(*p)) p++; /* read the string */ q = p; while (isalpha(*q)) q++; *q = '\0'; q++; if (triloop) { strncpy(s, p, 5); /*triloop string has 5 characters*/ s[5] = '\0'; } else { strncpy(s, p, 6); /*tetraloop string has 6 characters*/ s[6] = '\0'; } /* skip all spaces */ while (isspace(*q)) q++; p = q; while (!isspace(*p) && (*p != '\0')) p++; *p = '\0'; if (!strcmp(q, "inf")) *v = _INFINITY; else sscanf(q, "%lg", v); return 0; } static void getStack(double stackEntropies[5][5][5][5], double stackEnthalpies[5][5][5][5], thal_results* o) { int i, j, ii, jj; FILE *sFile, *hFile; sFile = openParamFile("stack.ds", o); hFile = openParamFile("stack.dh", o); for (i = 0; i < 5; ++i) { for (ii = 0; ii < 5; ++ii) { for (j = 0; j < 5; ++j) { for (jj = 0; jj < 5; ++jj) { if (i == 4 || j == 4 || ii == 4 || jj == 4) { stackEntropies[i][ii][j][jj] = -1.0; stackEnthalpies[i][ii][j][jj] = _INFINITY; } else { stackEntropies[i][ii][j][jj] = readDouble(sFile, o); stackEnthalpies[i][ii][j][jj] = readDouble(hFile, o); if (!isFinite(stackEntropies[i][ii][j][jj]) || !isFinite(stackEnthalpies[i][ii][j][jj])) { stackEntropies[i][ii][j][jj] = -1.0; stackEnthalpies[i][ii][j][jj] = _INFINITY; } } } } } } fclose(sFile); fclose(hFile); } static void getStackint2(double stackint2Entropies[5][5][5][5], double stackint2Enthalpies[5][5][5][5], thal_results* o) { int i, j, ii, jj; FILE *sFile, *hFile; sFile = openParamFile("stackmm.ds", o); hFile = openParamFile("stackmm.dh", o); for (i = 0; i < 5; ++i) { for (ii = 0; ii < 5; ++ii) { for (j = 0; j < 5; ++j) { for (jj = 0; jj < 5; ++jj) { if (i == 4 || j == 4 || ii == 4 || jj == 4) { stackint2Entropies[i][ii][j][jj] = -1.0; stackint2Enthalpies[i][ii][j][jj] = _INFINITY; } else { stackint2Entropies[i][ii][j][jj] = readDouble(sFile, o); stackint2Enthalpies[i][ii][j][jj] = readDouble(hFile, o); if (!isFinite(stackint2Entropies[i][ii][j][jj]) || !isFinite(stackint2Enthalpies[i][ii][j][jj])) { stackint2Entropies[i][ii][j][jj] = -1.0; stackint2Enthalpies[i][ii][j][jj] = _INFINITY; } } } } } } fclose(sFile); fclose(hFile); } /* static void verifyStackTable(double stack[5][5][5][5], char* type) { int i, j, ii, jj; for (i = 0; i < 4; ++i) for (j = 0; j < 4; ++j) for (ii = 0; ii < 4; ++ii) for (jj = 0; jj < 4; ++jj) if (stack[i][j][ii][jj] != stack[jj][ii][j][i]) #ifdef DEBUG fprintf(stderr, "Warning: symmetrical stacks _are_ _not_ equal: %c-%c/%c-%c stack %s is %g; %c-%c/%c-%c stack %s is %g\n", #endif BASES[i], BASES[j], BASES[ii], BASES[jj], type, stack[i][j][ii][jj], BASES[jj], BASES[ii], BASES[j], BASES[i], type, stack[jj][ii][j][i]); } */ static void getDangle(double dangleEntropies3[5][5][5], double dangleEnthalpies3[5][5][5], double dangleEntropies5[5][5][5], double dangleEnthalpies5[5][5][5], thal_results* o) { int i, j, k; FILE *sFile, *hFile; sFile = openParamFile("dangle.ds", o); hFile = openParamFile("dangle.dh", o); for (i = 0; i < 5; ++i) for (j = 0; j < 5; ++j) for (k = 0; k < 5; ++k) { if (i == 4 || j == 4) { dangleEntropies3[i][k][j] = -1.0; dangleEnthalpies3[i][k][j] = _INFINITY; } else if (k == 4) { dangleEntropies3[i][k][j] = -1.0; dangleEnthalpies3[i][k][j] = _INFINITY; } else { dangleEntropies3[i][k][j] = readDouble(sFile, o); dangleEnthalpies3[i][k][j] = readDouble(hFile, o); if(!isFinite(dangleEntropies3[i][k][j]) || !isFinite(dangleEnthalpies3[i][k][j])) { dangleEntropies3[i][k][j] = -1.0; dangleEnthalpies3[i][k][j] = _INFINITY; } } } for (i = 0; i < 5; ++i) for (j = 0; j < 5; ++j) for (k = 0; k < 5; ++k) { if (i == 4 || j == 4) { dangleEntropies5[i][j][k] = -1.0; dangleEnthalpies5[i][j][k] = _INFINITY; } else if (k == 4) { dangleEntropies5[i][j][k] = -1.0; dangleEnthalpies5[i][j][k] = _INFINITY; } else { dangleEntropies5[i][j][k] = readDouble(sFile, o); dangleEnthalpies5[i][j][k] = readDouble(hFile, o); if(!isFinite(dangleEntropies5[i][j][k]) || !isFinite(dangleEnthalpies5[i][j][k])) { dangleEntropies5[i][j][k] = -1.0; dangleEnthalpies5[i][j][k] = _INFINITY; } } } fclose(sFile); fclose(hFile); } static void getLoop(double hairpinLoopEntropies[30], double interiorLoopEntropies[30], double bulgeLoopEntropies[30], double hairpinLoopEnthalpies[30], double interiorLoopEnthalpies[30], double bulgeLoopEnthalpies[30], thal_results* o) { int k; FILE *sFile, *hFile; sFile = openParamFile("loops.ds", o); hFile = openParamFile("loops.dh", o); for (k = 0; k < 30; ++k) { readLoop(sFile, &interiorLoopEntropies[k], &bulgeLoopEntropies[k], &hairpinLoopEntropies[k], o); readLoop(hFile, &interiorLoopEnthalpies[k], &bulgeLoopEnthalpies[k], &hairpinLoopEnthalpies[k], o); } fclose(sFile); fclose(hFile); } static void getTstack(double tstackEntropies[5][5][5][5], double tstackEnthalpies[5][5][5][5], thal_results* o) { int i1, j1, i2, j2; FILE *sFile, *hFile; sFile = openParamFile("tstack_tm_inf.ds", o); hFile = openParamFile("tstack.dh", o); for (i1 = 0; i1 < 5; ++i1) for (i2 = 0; i2 < 5; ++i2) for (j1 = 0; j1 < 5; ++j1) for (j2 = 0; j2 < 5; ++j2) if (i1 == 4 || j1 == 4) { tstackEnthalpies[i1][i2][j1][j2] = _INFINITY; tstackEntropies[i1][i2][j1][j2] = -1.0; } else if (i2 == 4 || j2 == 4) { tstackEntropies[i1][i2][j1][j2] = 0.00000000001; tstackEnthalpies[i1][i2][j1][j2] = 0.0; } else { tstackEntropies[i1][i2][j1][j2] = readDouble(sFile, o); tstackEnthalpies[i1][i2][j1][j2] = readDouble(hFile, o); if (!isFinite(tstackEntropies[i1][i2][j1][j2]) || !isFinite(tstackEnthalpies[i1][i2][j1][j2])) { tstackEntropies[i1][i2][j1][j2] = -1.0; tstackEnthalpies[i1][i2][j1][j2] = _INFINITY; } } fclose(sFile); fclose(hFile); } static void getTstack2(double tstack2Entropies[5][5][5][5], double tstack2Enthalpies[5][5][5][5], thal_results* o) { int i1, j1, i2, j2; FILE *sFile, *hFile; sFile = openParamFile("tstack2.ds", o); hFile = openParamFile("tstack2.dh", o); for (i1 = 0; i1 < 5; ++i1) for (i2 = 0; i2 < 5; ++i2) for (j1 = 0; j1 < 5; ++j1) for (j2 = 0; j2 < 5; ++j2) if (i1 == 4 || j1 == 4) { tstack2Enthalpies[i1][i2][j1][j2] = _INFINITY; tstack2Entropies[i1][i2][j1][j2] = -1.0; } else if (i2 == 4 || j2 == 4) { tstack2Entropies[i1][i2][j1][j2] = 0.00000000001; tstack2Enthalpies[i1][i2][j1][j2] = 0.0; } else { tstack2Entropies[i1][i2][j1][j2] = readDouble(sFile, o); tstack2Enthalpies[i1][i2][j1][j2] = readDouble(hFile, o); if (!isFinite(tstack2Entropies[i1][i2][j1][j2]) || !isFinite(tstack2Enthalpies[i1][i2][j1][j2])) { tstack2Entropies[i1][i2][j1][j2] = -1.0; tstack2Enthalpies[i1][i2][j1][j2] = _INFINITY; } } fclose(sFile); fclose(hFile); } static void getTriloop(struct triloop** triloopEntropies, struct triloop** triloopEnthalpies, int* num, thal_results* o) { FILE *sFile, *hFile; int i, size; double value; sFile = openParamFile("triloop.ds", o); *num = 0; size = 16; *triloopEntropies = (struct triloop*) safe_calloc(16, sizeof(struct triloop), o); while (readTLoop(sFile, (*triloopEntropies)[*num].loop, &value, 1, o) != -1) { for (i = 0; i < 5; ++i) (*triloopEntropies)[*num].loop[i] = str2int((*triloopEntropies)[*num].loop[i]); (*triloopEntropies)[*num].value = value; ++*num; if (*num == size) { size *= 2; *triloopEntropies = (struct triloop*) safe_realloc(*triloopEntropies, size * sizeof(struct triloop), o); } } *triloopEntropies = (struct triloop*) safe_realloc(*triloopEntropies, *num * sizeof(struct triloop), o); fclose(sFile); hFile = openParamFile("triloop.dh", o); *num = 0; size = 16; *triloopEnthalpies = (struct triloop*) safe_calloc(16, sizeof(struct triloop), o); while (readTLoop(hFile, (*triloopEnthalpies)[*num].loop, &value, 1, o) != -1) { for (i = 0; i < 5; ++i) (*triloopEnthalpies)[*num].loop[i] = str2int((*triloopEnthalpies)[*num].loop[i]); (*triloopEnthalpies)[*num].value = value; ++*num; if (*num == size) { size *= 2; *triloopEnthalpies = (struct triloop*) safe_realloc(*triloopEnthalpies, size * sizeof(struct triloop), o); } } *triloopEnthalpies = (struct triloop*) safe_realloc(*triloopEnthalpies, *num * sizeof(struct triloop), o); fclose(hFile); } static void getTetraloop(struct tetraloop** tetraloopEntropies, struct tetraloop** tetraloopEnthalpies, int* num, thal_results* o) { FILE *sFile, *hFile; int i, size; double value; sFile = openParamFile("tetraloop.ds", o); *num = 0; size = 16; *tetraloopEntropies = (struct tetraloop*) safe_calloc(16, sizeof(struct tetraloop), o); while (readTLoop(sFile, (*tetraloopEntropies)[*num].loop, &value, 0, o) != -1) { for (i = 0; i < 6; ++i) (*tetraloopEntropies)[*num].loop[i] = str2int((*tetraloopEntropies)[*num].loop[i]); (*tetraloopEntropies)[*num].value = value; ++*num; if (*num == size) { size *= 2; *tetraloopEntropies = (struct tetraloop*) safe_realloc(*tetraloopEntropies, size * sizeof(struct tetraloop), o); } } *tetraloopEntropies = (struct tetraloop*) safe_realloc(*tetraloopEntropies, *num * sizeof(struct tetraloop), o); fclose(sFile); hFile = openParamFile("tetraloop.dh", o); *num = 0; size = 16; *tetraloopEnthalpies = (struct tetraloop*) safe_calloc(16, sizeof(struct tetraloop), o); while (readTLoop(hFile, (*tetraloopEnthalpies)[*num].loop, &value, 0, o) != -1) { for (i = 0; i < 6; ++i) (*tetraloopEnthalpies)[*num].loop[i] = str2int((*tetraloopEnthalpies)[*num].loop[i]); (*tetraloopEnthalpies)[*num].value = value; ++*num; if (*num == size) { size *= 2; *tetraloopEnthalpies = (struct tetraloop*) safe_realloc(*tetraloopEnthalpies, size * sizeof(struct tetraloop), o); } } *tetraloopEnthalpies = (struct tetraloop*) safe_realloc(*tetraloopEnthalpies, *num * sizeof(struct tetraloop), o); fclose(hFile); } static void tableStartATS(double atp_value, double atpS[5][5]) { int i, j; for (i = 0; i < 5; ++i) for (j = 0; j < 5; ++j) atpS[i][j] = 0.00000000001; atpS[0][3] = atpS[3][0] = atp_value; } static void tableStartATH(double atp_value, double atpH[5][5]) { int i, j; for (i = 0; i < 5; ++i) for (j = 0; j < 5; ++j) atpH[i][j] = 0.0; atpH[0][3] = atpH[3][0] = atp_value; } static int comp3loop(const void* loop1, const void* loop2) { int i; const unsigned char* h1 = (const unsigned char*) loop1; const struct triloop *h2 = (const struct triloop*) loop2; for (i = 0; i < 5; ++i) if (h1[i] < h2->loop[i]) return -1; else if (h1[i] > h2->loop[i]) return 1; return 0; } static int comp4loop(const void* loop1, const void* loop2) { int i; const unsigned char* h1 = (const unsigned char*) loop1; const struct tetraloop *h2 = (const struct tetraloop*) loop2; for (i = 0; i < 6; ++i) if (h1[i] < h2->loop[i]) return -1; else if (h1[i] > h2->loop[i]) return 1; return 0; } static void initMatrix() { int i, j; for (i = 1; i <= len1; ++i) { for (j = 1; j <= len2; ++j) { if (bpIndx(numSeq1[i], numSeq2[j]) == 0) { EnthalpyDPT(i, j) = _INFINITY; EntropyDPT(i, j) = -1.0; } else { EnthalpyDPT(i, j) = 0.0; EntropyDPT(i, j) = MinEntropy; } } } } static void initMatrix2() { int i, j; for (i = 1; i <= len1; ++i) for (j = i; j <= len2; ++j) if (j - i < MIN_HRPN_LOOP + 1 || (bpIndx(numSeq1[i], numSeq1[j]) == 0)) { EnthalpyDPT(i, j) = _INFINITY; EntropyDPT(i, j) = -1.0; } else { EnthalpyDPT(i, j) = 0.0; EntropyDPT(i, j) = MinEntropy; } } static void fillMatrix(int maxLoop, thal_results *o) { int d, i, j, ii, jj; double* SH; SH = (double*) safe_malloc(2 * sizeof(double), o); for (i = 1; i <= len1; ++i) { for (j = 1; j <= len2; ++j) { if(isFinite(EnthalpyDPT(i, j))) { /* if finite */ SH[0] = -1.0; SH[1] = _INFINITY; LSH(i,j,SH); if(isFinite(SH[1])) { EntropyDPT(i,j) = SH[0]; EnthalpyDPT(i,j) = SH[1]; } if (i > 1 && j > 1) { maxTM(i, j); /* stack: sets EntropyDPT(i, j) and EnthalpyDPT(i, j) */ for(d = 3; d <= maxLoop + 2; d++) { /* max=30, length over 30 is not allowed */ ii = i - 1; jj = - ii - d + (j + i); if (jj < 1) { ii -= abs(jj-1); jj = 1; } for (; ii > 0 && jj < j; --ii, ++jj) { if (isFinite(EnthalpyDPT(ii, jj))) { SH[0] = -1.0; SH[1] = _INFINITY; calc_bulge_internal(ii, jj, i, j, SH,0,maxLoop); if(SH[0] < MinEntropyCutoff) { /* to not give dH any value if dS is unreasonable */ SH[0] = MinEntropy; SH[1] = 0.0; } if(isFinite(SH[1])) { EnthalpyDPT(i, j) = SH[1]; EntropyDPT(i, j) = SH[0]; } } } } } /* if */ } } /* for */ } /* for */ free(SH); } static void fillMatrix2(int maxLoop, thal_results* o) { int i, j; double* SH; SH = (double*) safe_malloc(2 * sizeof(double), o); for (j = 2; j <= len2; ++j) for (i = j - MIN_HRPN_LOOP - 1; i >= 1; --i) { if (isFinite(EnthalpyDPT(i, j))) { SH[0] = -1.0; SH[1] = _INFINITY; maxTM2(i,j); /* calculate stack */ CBI(i, j, SH, 0,maxLoop); /* calculate Bulge and Internal loop and stack */ SH[0] = -1.0; SH[1] = _INFINITY; calc_hairpin(i, j, SH, 0); if(isFinite(SH[1])) { if(SH[0] < MinEntropyCutoff){ /* to not give dH any value if dS is unreasonable */ SH[0] = MinEntropy; SH[1] = 0.0; } EntropyDPT(i,j) = SH[0]; EnthalpyDPT(i,j) = SH[1]; } } } free(SH); } static void maxTM(int i, int j) { double T0, T1; double S0, S1; double H0, H1; T0 = T1 = -_INFINITY; S0 = EntropyDPT(i, j); H0 = EnthalpyDPT(i, j); T0 = (H0 + dplx_init_H) /(S0 + dplx_init_S + RC); /* at current position */ if(isFinite(EnthalpyDPT(i - 1, j - 1)) && isFinite(Hs(i - 1, j - 1, 1))) { S1 = (EntropyDPT(i - 1, j - 1) + Ss(i - 1, j - 1, 1)); H1 = (EnthalpyDPT(i - 1, j - 1) + Hs(i - 1, j - 1, 1)); } else { S1 = -1.0; H1 = _INFINITY; } T1 = (H1 + dplx_init_H) /(S1 + dplx_init_S + RC); if(S1 < MinEntropyCutoff) { /* to not give dH any value if dS is unreasonable */ S1 = MinEntropy; H1 = 0.0; } if(S0 < MinEntropyCutoff) { /* to not give dH any value if dS is unreasonable */ S0 = MinEntropy; H0 = 0.0; } if((T1 > T0) || (S0>0 && H0>0)){ /* T1 on suurem */ EntropyDPT(i, j) = S1; EnthalpyDPT(i, j) = H1; } else if(T0 >= T1) { EntropyDPT(i, j) = S0; EnthalpyDPT(i, j) = H0; } } static void maxTM2(int i, int j) { double T0, T1; double S0, S1; double H0, H1; T0 = T1 = -_INFINITY; S0 = EntropyDPT(i, j); H0 = EnthalpyDPT(i, j); T0 = (H0 + dplx_init_H) /(S0 + dplx_init_S + RC); if(isFinite(EnthalpyDPT(i, j))) { S1 = (EntropyDPT(i + 1, j - 1) + Ss(i, j, 2)); H1 = (EnthalpyDPT(i + 1, j - 1) + Hs(i, j, 2)); } else { S1 = -1.0; H1 = _INFINITY; } T1 = (H1 + dplx_init_H) /(S1 + dplx_init_S + RC); if(S1 < MinEntropyCutoff) { S1 = MinEntropy; H1 = 0.0; } if(S0 < MinEntropyCutoff) { S0 = MinEntropy; H0 = 0.0; } if(T1 > T0) { EntropyDPT(i, j) = S1; EnthalpyDPT(i, j) = H1; } else { EntropyDPT(i, j) = S0; EnthalpyDPT(i, j) = H0; } } static void LSH(int i, int j, double* EntropyEnthalpy) { double S1, H1, T1; double S2, H2, T2; S1 = S2 = -1.0; H1 = H2 = -_INFINITY; T1 = T2 = -_INFINITY; if (bpIndx(numSeq1[i], numSeq2[j]) == 0) { EntropyDPT(i, j) = -1.0; EnthalpyDPT(i, j) = _INFINITY; return; } S1 = atPenaltyS(numSeq1[i], numSeq2[j]) + tstack2Entropies[numSeq2[j]][numSeq2[j-1]][numSeq1[i]][numSeq1[i-1]]; H1 = atPenaltyH(numSeq1[i], numSeq2[j]) + tstack2Enthalpies[numSeq2[j]][numSeq2[j-1]][numSeq1[i]][numSeq1[i-1]]; if(!isFinite(H1)) { H1 = _INFINITY; S1 = -1.0; } /** If there is two dangling ends at the same end of duplex **/ if(isFinite(dangleEnthalpies3[numSeq2[j]][numSeq2[j - 1]][numSeq1[i]]) && isFinite(dangleEnthalpies5[numSeq2[j]][numSeq1[i]][numSeq1[i - 1]])) { S2 = atPenaltyS(numSeq1[i], numSeq2[j]) + dangleEntropies3[numSeq2[j]][numSeq2[j - 1]][numSeq1[i]] + dangleEntropies5[numSeq2[j]][numSeq1[i]][numSeq1[i - 1]]; H2 = atPenaltyH(numSeq1[i], numSeq2[j]) + dangleEnthalpies3[numSeq2[j]][numSeq2[j - 1]][numSeq1[i]] + dangleEnthalpies5[numSeq2[j]][numSeq1[i]][numSeq1[i - 1]]; if(!isFinite(H2)) { H2 = _INFINITY; S2 = -1.0; } T2 = (H2 + dplx_init_H) / (S2 + dplx_init_S + RC); if(isFinite(H1)) { T1 = (H1 + dplx_init_H) / (S1 + dplx_init_S + RC); if(T1= j) return -1.0; if (i == len1 || j == len2 + 1) return -1.0; if (i > len1) i -= len1; if (j > len2) j -= len2; return stackEntropies[numSeq1[i]][numSeq1[i+1]][numSeq2[j]][numSeq2[j-1]]; } else { return stackEntropies[numSeq1[i]][numSeq1[i + 1]][numSeq2[j]][numSeq2[j + 1]]; } } static double Hs(int i, int j, int k) { if(k==2) { if (i >= j) return _INFINITY; if (i == len1 || j == len2 + 1) return _INFINITY; if (i > len1) i -= len1; if (j > len2) j -= len2; if(isFinite(stackEnthalpies[numSeq1[i]][numSeq1[i+1]][numSeq2[j]][numSeq2[j-1]])) { return stackEnthalpies[numSeq1[i]][numSeq1[i+1]][numSeq2[j]][numSeq2[j-1]]; } else { return _INFINITY; } } else { return stackEnthalpies[numSeq1[i]][numSeq1[i + 1]][numSeq2[j]][numSeq2[j + 1]]; } } static void CBI(int i, int j, double* EntropyEnthalpy, int traceback, int maxLoop) { int d, ii, jj; for (d = j - i - 3; d >= MIN_HRPN_LOOP + 1 && d >= j - i - 2 - maxLoop; --d) for (ii = i + 1; ii < j - d && ii <= len1; ++ii) { jj = d + ii; if(traceback==0) { EntropyEnthalpy[0] = -1.0; EntropyEnthalpy[1] = _INFINITY; } if (isFinite(EnthalpyDPT(ii, jj))) { calc_bulge_internal2(i, j, ii, jj, EntropyEnthalpy, traceback,maxLoop); if(isFinite(EntropyEnthalpy[1])) { if(EntropyEnthalpy[0] < MinEntropyCutoff) { EntropyEnthalpy[0] = MinEntropy; EntropyEnthalpy[1] = 0.0; } if(traceback==0) { EnthalpyDPT(i, j) = EntropyEnthalpy[1]; EntropyDPT(i, j) = EntropyEnthalpy[0]; } } } } return; } static void calc_hairpin(int i, int j, double* EntropyEnthalpy, int traceback) { int loopSize = j - i - 1; double T1, T2; T1 = T2 = -_INFINITY; if(loopSize < MIN_HRPN_LOOP) { EntropyEnthalpy[0] = -1.0; EntropyEnthalpy[1] = _INFINITY; return; } if (i <= len1 && len2 < j) { EntropyEnthalpy[0] = -1.0; EntropyEnthalpy[1] = _INFINITY; return; } else if (i > len2) { i -= len1; j -= len2; } if(loopSize <= 30) { EntropyEnthalpy[1] = hairpinLoopEnthalpies[loopSize - 1]; EntropyEnthalpy[0] = hairpinLoopEntropies[loopSize - 1]; } else { EntropyEnthalpy[1] = hairpinLoopEnthalpies[29]; EntropyEnthalpy[0] = hairpinLoopEntropies[29]; } if (loopSize > 3) { /* for loops 4 bp and more in length, terminal mm are accounted */ EntropyEnthalpy[1] += tstack2Enthalpies[numSeq1[i]][numSeq1[i + 1]][numSeq1[j]][numSeq1[j - 1]]; EntropyEnthalpy[0] += tstack2Entropies[numSeq1[i]][numSeq1[i + 1]][numSeq1[j]][numSeq1[j - 1]]; } else if(loopSize == 3){ /* for loops 3 bp in length at-penalty is considered */ EntropyEnthalpy[1] += atPenaltyH(numSeq1[i], numSeq1[j]); EntropyEnthalpy[0] += atPenaltyS(numSeq1[i], numSeq1[j]); } if (loopSize == 3) { /* closing AT-penalty (+), triloop bonus, hairpin of 3 (+) */ struct triloop* loop; if (numTriloops) { if ((loop = (struct triloop*) bsearch(numSeq1 + i, triloopEnthalpies, numTriloops, sizeof(struct triloop), comp3loop))) EntropyEnthalpy[1] += loop->value; if ((loop = (struct triloop*) bsearch(numSeq1 + i, triloopEntropies, numTriloops, sizeof(struct triloop), comp3loop))) EntropyEnthalpy[0] += loop->value; } } else if (loopSize == 4) { /* terminal mismatch, tetraloop bonus, hairpin of 4 */ struct tetraloop* loop; if (numTetraloops) { if ((loop = (struct tetraloop*) bsearch(numSeq1 + i, tetraloopEnthalpies, numTetraloops, sizeof(struct tetraloop), comp4loop))) { EntropyEnthalpy[1] += loop->value; } if ((loop = (struct tetraloop*) bsearch(numSeq1 + i, tetraloopEntropies, numTetraloops, sizeof(struct tetraloop), comp4loop))) { EntropyEnthalpy[0] += loop->value; } } } if(!isFinite(EntropyEnthalpy[1])) { EntropyEnthalpy[1] = _INFINITY; EntropyEnthalpy[0] = -1.0; } if(isPositive(EntropyEnthalpy[1]) && isPositive(EntropyEnthalpy[0]) && (!isPositive(EnthalpyDPT(i, j)) || !isPositive(EntropyDPT(i, j)))) { /* if both, S and H are positive */ EntropyEnthalpy[1] = _INFINITY; EntropyEnthalpy[0] = -1.0; } T1 = (EntropyEnthalpy[1] + dplx_init_H) / ((EntropyEnthalpy[0] + dplx_init_S + RC)); T2 = (EnthalpyDPT(i, j) + dplx_init_H) / ((EntropyDPT(i, j)) + dplx_init_S + RC); if(T1 < T2 && traceback == 0) { EntropyEnthalpy[0] = EntropyDPT(i, j); EntropyEnthalpy[1] = EnthalpyDPT(i, j); } return; } static void calc_bulge_internal(int i, int j, int ii, int jj, double* EntropyEnthalpy, int traceback, int maxLoop) { int loopSize1, loopSize2, loopSize; double T1, T2; double S,H; int N, N_loop; T1 = T2 = -_INFINITY; S = MinEntropy; H = 0; loopSize1 = ii - i - 1; loopSize2 = jj - j - 1; if(ii < jj) { N = ((2 * i)/2); N_loop = N; if(loopSize1 > 2) N_loop -= (loopSize1 - 2); if(loopSize2 > 2) N_loop -= (loopSize2 - 2); } else { N = ((2 * j)/2); N_loop = 2 * jj; if(loopSize1 > 2) N_loop -= (loopSize1 - 2); if(loopSize2 > 2) N_loop -= (loopSize2 - 2); N_loop = (N_loop/2) - 1; } #ifdef DEBUG if (ii <= i){ fputs("Error in calc_bulge_internal(): ii is not greater than i\n", stderr); } if (jj <= j) fputs("Error in calc_bulge_internal(): jj is not greater than j\n", stderr); #endif #ifdef DEBUG if (loopSize1 + loopSize2 > maxLoop) { fputs("Error: calc_bulge_internal() called with loopSize1 + loopSize2 > maxLoop\n", stderr); return; } #endif #ifdef DEBUG if (loopSize1 == 0 && loopSize2 == 0) { fputs("Error: calc_bulge_internal() called with nonsense\n", stderr); return; } #endif loopSize = loopSize1 + loopSize2 -1; if((loopSize1 == 0 && loopSize2 > 0) || (loopSize2 == 0 && loopSize1 > 0)) { /* only bulges have to be considered */ if(loopSize2 == 1 || loopSize1 == 1) { /* bulge loop of size one is treated differently the intervening nn-pair must be added */ if((loopSize2 == 1 && loopSize1 == 0) || (loopSize2 == 0 && loopSize1 == 1)) { H = bulgeLoopEnthalpies[loopSize] + stackEnthalpies[numSeq1[i]][numSeq1[ii]][numSeq2[j]][numSeq2[jj]]; S = bulgeLoopEntropies[loopSize] + stackEntropies[numSeq1[i]][numSeq1[ii]][numSeq2[j]][numSeq2[jj]]; } H += EnthalpyDPT(i, j); S += EntropyDPT(i, j); if(!isFinite(H)) { H = _INFINITY; S = -1.0; } if(isPositive(H) && isPositive(S) && (!isPositive(EnthalpyDPT(ii, jj)) || !isPositive(EntropyDPT(ii, jj)))) { /* if both, S and H are positive */ H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / ((S + dplx_init_S) + RC); T2 = (EnthalpyDPT(ii, jj) + dplx_init_H) / ((EntropyDPT(ii, jj)) + dplx_init_S + RC); if((T1 > T2) || ((traceback && T1 >= T2) || (traceback==1))) { EntropyEnthalpy[0] = S; EntropyEnthalpy[1] = H; } } else { /* we have _not_ implemented Jacobson-Stockaymayer equation; the maximum bulgeloop size is 30 */ H = bulgeLoopEnthalpies[loopSize] + atPenaltyH(numSeq1[i], numSeq2[j]) + atPenaltyH(numSeq1[ii], numSeq2[jj]); H += EnthalpyDPT(i, j); S = bulgeLoopEntropies[loopSize] + atPenaltyS(numSeq1[i], numSeq2[j]) + atPenaltyS(numSeq1[ii], numSeq2[jj]); S += EntropyDPT(i, j); if(!isFinite(H)) { H = _INFINITY; S = -1.0; } if(isPositive(H) && isPositive(S) && (!isPositive(EnthalpyDPT(i, j)) || !isPositive(EntropyDPT(i, j)))){ /* if both, S and H are positive */ H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / ((S + dplx_init_S) + RC); T2 = (EnthalpyDPT(ii, jj) + dplx_init_H) / (EntropyDPT(ii, jj) + dplx_init_S + RC); if((T1 > T2) || ((traceback && T1 >= T2) || (traceback==1))) { EntropyEnthalpy[0] = S; EntropyEnthalpy[1] = H; } } } else if (loopSize1 == 1 && loopSize2 == 1) { S = stackint2Entropies[numSeq1[i]][numSeq1[i+1]][numSeq2[j]][numSeq2[j+1]] + stackint2Entropies[numSeq2[jj]][numSeq2[jj-1]][numSeq1[ii]][numSeq1[ii-1]]; S += EntropyDPT(i, j); H = stackint2Enthalpies[numSeq1[i]][numSeq1[i+1]][numSeq2[j]][numSeq2[j+1]] + stackint2Enthalpies[numSeq2[jj]][numSeq2[jj-1]][numSeq1[ii]][numSeq1[ii-1]]; H += EnthalpyDPT(i, j); if(!isFinite(H)) { H = _INFINITY; S = -1.0; } if(isPositive(H) && isPositive(S) && (!isPositive(EnthalpyDPT(ii, jj)) || !isPositive(EntropyDPT(ii, jj)))) { /* if both, S and H are positive */ H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / ((S + dplx_init_S) + RC); T2 = (EnthalpyDPT(ii, jj) + dplx_init_H) / (EntropyDPT(ii, jj) + dplx_init_S + RC); if((DBL_EQ(T1,T2) == 2) || traceback==1) { if((T1 > T2) || (traceback && T1 >= T2)) { EntropyEnthalpy[0] = S; EntropyEnthalpy[1] = H; } } return; } else { /* only internal loops */ H = interiorLoopEnthalpies[loopSize] + tstackEnthalpies[numSeq1[i]][numSeq1[i+1]][numSeq2[j]][numSeq2[j+1]] + tstackEnthalpies[numSeq2[jj]][numSeq2[jj-1]][numSeq1[ii]][numSeq1[ii-1]] + (ILAH * abs(loopSize1 - loopSize2)); H += EnthalpyDPT(i, j); S = interiorLoopEntropies[loopSize] + tstackEntropies[numSeq1[i]][numSeq1[i+1]][numSeq2[j]][numSeq2[j+1]] + tstackEntropies[numSeq2[jj]][numSeq2[jj-1]][numSeq1[ii]][numSeq1[ii-1]] + (ILAS * abs(loopSize1 - loopSize2)); S += EntropyDPT(i, j); if(!isFinite(H)) { H = _INFINITY; S = -1.0; } if(isPositive(H) && isPositive(S) && (!isPositive(EnthalpyDPT(ii, jj)) || !isPositive(EntropyDPT(ii, jj)))) { /* if both, S and H are positive */ H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / ((S + dplx_init_S) + RC); T2 = (EnthalpyDPT(ii, jj) + dplx_init_H) / ((EntropyDPT(ii, jj)) + dplx_init_S + RC); if((T1 > T2) || ((traceback && T1 >= T2) || (traceback==1))) { EntropyEnthalpy[0] = S; EntropyEnthalpy[1] = H; } } return; } static void calc_bulge_internal2(int i, int j, int ii, int jj, double* EntropyEnthalpy, int traceback, int maxLoop) { int loopSize1, loopSize2, loopSize; double T1, T2; double S,H; /* int N, N_loop; Triinu, please review */ T1 = T2 = -_INFINITY; S = MinEntropy; H = 0.0; loopSize1 = ii - i - 1; loopSize2 = j - jj - 1; if (loopSize1 + loopSize2 > maxLoop) { EntropyEnthalpy[0] = -1.0; EntropyEnthalpy[1] = _INFINITY; return; } /* Triinu, please review the statements below. */ if(i < (len1 -j)) { /* N = i; */ /* N_loop = (i - 1); */ } else { /* N = len1-j; */ /* N_loop = len1 - j - 1; */ } #ifdef DEBUG if (ii <= i) fputs("Error in calc_bulge_internal(): ii isn't greater than i\n", stderr); if (jj >= j) fputs("Error in calc_bulge_internal(): jj isn't less than j\n", stderr); if (ii >= jj) fputs("Error in calc_bulge_internal(): jj isn't greater than ii\n", stderr); if ((i <= len1 && len1 < ii) || (jj <= len2 && len2 < j)) { EntropyEnthalpy[0] = -1.0; EntropyEnthalpy[1] = _INFINITY; return; } #endif #ifdef DEBUG if (loopSize1 + loopSize2 > maxLoop) { fputs("Error: calc_bulge_internal() called with loopSize1 + loopSize2 > maxLoop\n", stderr); return; } #endif #ifdef DEBUG if (loopSize1 == 0 && loopSize2 == 0) { fputs("Error: calc_bulge_internal() called with nonsense\n", stderr); return; } #endif #ifdef DEBUG if (i > len1) i -= len1; if (ii > len1) ii -= len1; if (j > len2) j -= len2; if (jj > len2) jj -= len2; #endif loopSize = loopSize1 + loopSize2 -1; /* for indx only */ if((loopSize1 == 0 && loopSize2 > 0) || (loopSize2 == 0 && loopSize1 > 0)) { /* only bulges have to be considered */ if(loopSize2 == 1 || loopSize1 == 1) { /* bulge loop of size one is treated differently the intervening nn-pair must be added */ if((loopSize2 == 1 && loopSize1 == 0) || (loopSize2 == 0 && loopSize1 == 1)) { H = bulgeLoopEnthalpies[loopSize] + stackEnthalpies[numSeq1[i]][numSeq1[ii]][numSeq2[j]][numSeq2[jj]]; S = bulgeLoopEntropies[loopSize] + stackEntropies[numSeq1[i]][numSeq1[ii]][numSeq2[j]][numSeq2[jj]]; } if(traceback!=1) { H += EnthalpyDPT(ii, jj); /* bulge koos otsaga, st bulge i,j-ni */ S += EntropyDPT(ii, jj); } if(!isFinite(H)) { H = _INFINITY; S = -1.0; } if(isPositive(H) && isPositive(S) && (!isPositive(EnthalpyDPT(i, j)) || !isPositive(EntropyDPT(i, j)))) { /* if both, S and H are positive */ H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / ((S + dplx_init_S) + RC); T2 = (EnthalpyDPT(i, j) + dplx_init_H) / ((EntropyDPT(i, j)) + dplx_init_S + RC); if((T1 > T2) || ((traceback && T1 >= T2) || traceback==1)) { EntropyEnthalpy[0] = S; EntropyEnthalpy[1] = H; } } else { /* we have _not_ implemented Jacobson-Stockaymayer equation; the maximum bulgeloop size is 30 */ H = bulgeLoopEnthalpies[loopSize] + atPenaltyH(numSeq1[i], numSeq2[j]) + atPenaltyH(numSeq1[ii], numSeq2[jj]); if(traceback!=1) H += EnthalpyDPT(ii, jj); S = bulgeLoopEntropies[loopSize] + atPenaltyS(numSeq1[i], numSeq2[j]) + atPenaltyS(numSeq1[ii], numSeq2[jj]); if(traceback!=1) S += EntropyDPT(ii, jj); if(!isFinite(H)) { H = _INFINITY; S = -1.0; } if(isPositive(H) && isPositive(S) && (!isPositive(EnthalpyDPT(i, j)) || !isPositive(EntropyDPT(i, j)))) { /* if both, S and H are positive */ H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / ((S + dplx_init_S) + RC); T2 = (EnthalpyDPT(i, j) + dplx_init_H) / (EntropyDPT(i, j) + dplx_init_S + RC); if((T1 > T2) || ((traceback && T1 >= T2) || (traceback==1))) { EntropyEnthalpy[0] = S; EntropyEnthalpy[1] = H; } } } /* end of calculating bulges */ else if (loopSize1 == 1 && loopSize2 == 1) { /* mismatch nearest neighbor parameters */ S = stackint2Entropies[numSeq1[i]][numSeq1[i+1]][numSeq2[j]][numSeq2[j-1]] + stackint2Entropies[numSeq2[jj]][numSeq2[jj+1]][numSeq1[ii]][numSeq1[ii-1]]; if(traceback!=1) S += EntropyDPT(ii, jj); H = stackint2Enthalpies[numSeq1[i]][numSeq1[i+1]][numSeq2[j]][numSeq2[j-1]] + stackint2Enthalpies[numSeq2[jj]][numSeq2[jj+1]][numSeq1[ii]][numSeq1[ii-1]]; if(traceback!=1) H += EnthalpyDPT(ii, jj); if(!isFinite(H)) { H = _INFINITY; S = -1.0; } if(isPositive(H)==1 && isPositive(S)==1 && (!isPositive(EnthalpyDPT(i, j)) || !isPositive(EntropyDPT(i, j)))) { /* if both, S and H are positive */ H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / ((S + dplx_init_S) + RC); T2 = (EnthalpyDPT(i, j) + dplx_init_H) / (EntropyDPT(i, j) + dplx_init_S + RC); if((DBL_EQ(T1,T2) == 2) || traceback) { if((T1 > T2) || ((traceback && T1 >= T2) || traceback==1)) { EntropyEnthalpy[0] = S; EntropyEnthalpy[1] = H; } } return; } else { /* only internal loops */ H = interiorLoopEnthalpies[loopSize] + tstackEnthalpies[numSeq1[i]][numSeq1[i+1]][numSeq2[j]][numSeq2[j-1]] + tstackEnthalpies[numSeq2[jj]][numSeq2[jj+1]][numSeq1[ii]][numSeq1[ii-1]] + (ILAH * abs(loopSize1 - loopSize2)); if(traceback!=1) H += EnthalpyDPT(ii, jj); S = interiorLoopEntropies[loopSize] + tstackEntropies[numSeq1[i]][numSeq1[i+1]][numSeq2[j]][numSeq2[j-1]] + tstackEntropies[numSeq2[jj]][numSeq2[jj+1]][numSeq1[ii]][numSeq1[ii-1]] + (ILAS * abs(loopSize1 - loopSize2)); if(traceback!=1) S += EntropyDPT(ii, jj); if(!isFinite(H)) { H = _INFINITY; S = -1.0; } if(isPositive(H) && isPositive(S) && (!isPositive(EnthalpyDPT(i, j)) || !isPositive(EntropyDPT(i, j)))){ /* if both, S and H are positive */ H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / ((S + dplx_init_S) + RC); T2 = (EnthalpyDPT(i, j) + dplx_init_H) / ((EntropyDPT(i, j)) + dplx_init_S + RC); if((T1 > T2) || ((traceback && T1 >= T2) || (traceback==1))) { EntropyEnthalpy[0] = S; EntropyEnthalpy[1] = H; } } return; } static void calc_terminal_bp(double temp) { /* compute exterior loop */ int i; int max; SEND5(0) = SEND5(1) = -1.0; HEND5(0) = HEND5(1) = _INFINITY; for(i = 2; i<=(len1); i++) { SEND5(i) = MinEntropy; HEND5(i) = 0; } double T1, T2, T3, T4, T5; T1 = T2 = T3 = T4 = T5 = -_INFINITY; double G; /* adding terminal penalties to 3' end and to 5' end */ for(i = 2; i <= len1; ++i) { max = 0; T1 = T2 = T3 = T4 = T5 = -_INFINITY; T1 = (HEND5(i - 1) + dplx_init_H) / (SEND5(i - 1) + dplx_init_S + RC); T2 = (END5_1(i,1) + dplx_init_H) / (END5_1(i,2) + dplx_init_S + RC); T3 = (END5_2(i,1) + dplx_init_H) / (END5_2(i,2) + dplx_init_S + RC); T4 = (END5_3(i,1) + dplx_init_H) / (END5_3(i,2) + dplx_init_S + RC); T5 = (END5_4(i,1) + dplx_init_H) / (END5_4(i,2) + dplx_init_S + RC); max = max5(T1,T2,T3,T4,T5); switch (max) { case 1: SEND5(i) = SEND5(i - 1); HEND5(i) = HEND5(i - 1); break; case 2: G = END5_1(i,1) - (temp * (END5_1(i,2))); if(G < G2) { SEND5(i) = END5_1(i,2); HEND5(i) = END5_1(i,1); } else { SEND5(i) = SEND5(i - 1); HEND5(i) = HEND5(i - 1); } break; case 3: G = END5_2(i,1) - (temp * (END5_2(i,2))); if(G < G2) { SEND5(i) = END5_2(i,2); HEND5(i) = END5_2(i,1); } else { SEND5(i) = SEND5(i - 1); HEND5(i) = HEND5(i - 1); } break; case 4: G = END5_3(i,1) - (temp * (END5_3(i,2))); if(G < G2) { SEND5(i) = END5_3(i,2); HEND5(i) = END5_3(i,1); } else { SEND5(i) = SEND5(i - 1); HEND5(i) = HEND5(i - 1); } break; case 5: G = END5_4(i,1) - (temp * (END5_4(i,2))); if(G < G2) { SEND5(i) = END5_4(i,2); HEND5(i) = END5_4(i,1); } else { SEND5(i) = SEND5(i - 1); HEND5(i) = HEND5(i - 1); } break; default: #ifdef DEBUG printf ("WARNING: max5 returned character code %d ??\n", max); #endif break; } } } static double END5_1(int i,int hs) { int k; double max_tm; /* energy min */ double T1, T2; double H, S; double H_max, S_max; H_max = H = _INFINITY; S_max = S = -1.0; T1 = T2 = -_INFINITY; max_tm = -_INFINITY; for(k = 0; k <= i - MIN_HRPN_LOOP - 2; ++k) { T1 = (HEND5(k) + dplx_init_H) /(SEND5(k) + dplx_init_S + RC); T2 = (0 + dplx_init_H) /(0 + dplx_init_S + RC); if(T1 >= T2) { H = HEND5(k) + atPenaltyH(numSeq1[k + 1], numSeq1[i]) + EnthalpyDPT(k + 1, i); S = SEND5(k) + atPenaltyS(numSeq1[k + 1], numSeq1[i]) + EntropyDPT(k + 1, i); if(!isFinite(H) || H > 0 || S > 0) { /* H and S must be greater than 0 to avoid BS */ H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / (S + dplx_init_S + RC); } else { H = 0 + atPenaltyH(numSeq1[k + 1], numSeq1[i]) + EnthalpyDPT(k + 1, i); S = 0 + atPenaltyS(numSeq1[k + 1], numSeq1[i]) + EntropyDPT(k + 1, i); if(!isFinite(H) || H > 0 || S > 0) { H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) /(S + dplx_init_S + RC); } if(max_tm < T1) { if(S > MinEntropyCutoff) { H_max = H; S_max = S; max_tm = T1; } } } if (hs == 1) return H_max; return S_max; } static double END5_2(int i,int hs) { int k; double max_tm; double T1, T2; double H, S; double H_max, S_max; H_max = H = _INFINITY; T1 = T2 = max_tm = -_INFINITY; S_max = S = -1.0; for (k = 0; k <= i - MIN_HRPN_LOOP - 3; ++k) { T1 = (HEND5(k) + dplx_init_H) /(SEND5(k) + dplx_init_S + RC); T2 = (0 + dplx_init_H) /(0 + dplx_init_S + RC); if(T1 >= T2) { H = HEND5(k) + atPenaltyH(numSeq1[k + 2], numSeq1[i]) + Hd5(i, k + 2) + EnthalpyDPT(k + 2, i); S = SEND5(k) + atPenaltyS(numSeq1[k + 2], numSeq1[i]) + Sd5(i, k + 2) + EntropyDPT(k + 2, i); if(!isFinite(H) || H > 0 || S > 0) { H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / (S + dplx_init_S + RC); } else { H = 0 + atPenaltyH(numSeq1[k + 2], numSeq1[i]) + Hd5(i, k + 2) + EnthalpyDPT(k + 2, i); S = 0 + atPenaltyS(numSeq1[k + 2], numSeq1[i]) + Sd5(i, k + 2) + EntropyDPT(k + 2, i); if(!isFinite(H) || H > 0 || S > 0) { H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) /(S + dplx_init_S + RC); } if(max_tm < T1) { if(S > MinEntropyCutoff) { H_max = H; S_max = S; max_tm = T1; } } } if (hs == 1) return H_max; return S_max; } static double END5_3(int i,int hs) { int k; double max_tm; double T1, T2; double H, S; double H_max, S_max; H_max = H = _INFINITY;; T1 = T2 = max_tm = -_INFINITY; S_max = S = -1.0; for (k = 0; k <= i - MIN_HRPN_LOOP - 3; ++k) { T1 = (HEND5(k) + dplx_init_H) /(SEND5(k) + dplx_init_S + RC); T2 = (0 + dplx_init_H) /(0 + dplx_init_S + RC); if(T1 >= T2) { H = HEND5(k) + atPenaltyH(numSeq1[k + 1], numSeq1[i - 1]) + Hd3(i - 1, k + 1) + EnthalpyDPT(k + 1, i - 1); S = SEND5(k) + atPenaltyS(numSeq1[k + 1], numSeq1[i - 1]) + Sd3(i - 1, k + 1) + EntropyDPT(k + 1, i - 1); if(!isFinite(H) || H > 0 || S > 0) { H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / (S + dplx_init_S + RC); } else { H = 0 + atPenaltyH(numSeq1[k + 1], numSeq1[i - 1]) + Hd3(i - 1, k + 1) + EnthalpyDPT(k + 1, i - 1); S = 0 + atPenaltyS(numSeq1[k + 1], numSeq1[i - 1]) + Sd3(i - 1, k + 1) + EntropyDPT(k + 1, i - 1); if(!isFinite(H) || H > 0 || S > 0) { H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) /(S + dplx_init_S + RC); } if(max_tm < T1) { if(S > MinEntropyCutoff) { H_max = H; S_max = S; max_tm = T1; } } } if (hs == 1) return H_max; return S_max; } static double END5_4(int i,int hs) { int k; double max_tm; double T1, T2; double H, S; double H_max, S_max; H_max = H = _INFINITY; T1 = T2 = max_tm = -_INFINITY; S_max = S = -1.0; for(k = 0; k <= i - MIN_HRPN_LOOP - 4; ++k) { T1 = (HEND5(k) + dplx_init_H) /(SEND5(k) + dplx_init_S + RC); T2 = (0 + dplx_init_H) /(0 + dplx_init_S + RC); if(T1 >= T2) { H = HEND5(k) + atPenaltyH(numSeq1[k + 2], numSeq1[i - 1]) + Htstack(i - 1, k + 2) + EnthalpyDPT(k + 2, i - 1); S = SEND5(k) + atPenaltyS(numSeq1[k + 2], numSeq1[i - 1]) + Ststack(i - 1, k + 2) + EntropyDPT(k + 2, i - 1); if(!isFinite(H) || H > 0 || S > 0) { H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) / (S + dplx_init_S + RC); } else { H = 0 + atPenaltyH(numSeq1[k + 2], numSeq1[i - 1]) + Htstack(i - 1, k + 2) + EnthalpyDPT(k + 2, i - 1); S = 0 + atPenaltyS(numSeq1[k + 2], numSeq1[i - 1]) + Ststack(i - 1, k + 2) + EntropyDPT(k + 2, i - 1); if(!isFinite(H) || H > 0 || S > 0) { H = _INFINITY; S = -1.0; } T1 = (H + dplx_init_H) /(S + dplx_init_S + RC); } if(max_tm < T1) { if(S > MinEntropyCutoff) { H_max = H; S_max = S; max_tm = T1; } } } if (hs == 1) return H_max; return S_max; } static double Sd5(int i, int j) { return dangleEntropies5[numSeq1[i]][numSeq1[j]][numSeq1[j - 1]]; } static double Hd5(int i, int j) { return dangleEnthalpies5[numSeq1[i]][numSeq1[j]][numSeq1[j - 1]]; } static double Sd3(int i, int j) { return dangleEntropies3[numSeq1[i]][numSeq1[i+1]][numSeq1[j]]; } static double Hd3(int i, int j) { return dangleEnthalpies3[numSeq1[i]][numSeq1[i+1]][numSeq1[j]]; } static double Ststack(int i, int j) { return tstack2Entropies[numSeq1[i]][numSeq1[i+1]][numSeq1[j]][numSeq1[j-1]]; } static double Htstack(int i, int j) { /* e.g AG_TC 210 */ return tstack2Enthalpies[numSeq1[i]][numSeq1[i+1]][numSeq1[j]][numSeq1[j-1]]; } /* Return 1 if string is symmetrical, 0 otherwise. */ static int symmetry_thermo(const unsigned char* seq) { register char s; register char e; const unsigned char *seq_end=seq; int i = 0; int seq_len=length_unsig_char(seq); int mp = seq_len/2; if(seq_len%2==1) { return 0; } seq_end+=seq_len; seq_end--; while(inext; i = top->i; j = top->j; if(top->mtrx==1) { while (equal(SEND5(i), SEND5(i - 1)) && equal(HEND5(i), HEND5(i - 1))) /* if previous structure is the same as this one */ --i; if (i == 0) continue; if (equal(SEND5(i), END5_1(i,2)) && equal(HEND5(i), END5_1(i,1))) { for (k = 0; k <= i - MIN_HRPN_LOOP - 2; ++k) if (equal(SEND5(i), atPenaltyS(numSeq1[k + 1], numSeq1[i]) + EntropyDPT(k + 1, i)) && equal(HEND5(i), atPenaltyH(numSeq1[k + 1], numSeq1[i]) + EnthalpyDPT(k + 1, i))) { push(&stack, k + 1, i,0, o); break; } else if (equal(SEND5(i), SEND5(k) + atPenaltyS(numSeq1[k + 1], numSeq1[i]) + EntropyDPT(k + 1, i)) && equal(HEND5(i), HEND5(k) + atPenaltyH(numSeq1[k + 1], numSeq1[i]) + EnthalpyDPT(k + 1, i))) { push(&stack, k + 1, i, 0, o); push(&stack, k, 0, 1, o); break; } } else if (equal(SEND5(i), END5_2(i,2)) && equal(HEND5(i), END5_2(i,1))) { for (k = 0; k <= i - MIN_HRPN_LOOP - 3; ++k) if (equal(SEND5(i), atPenaltyS(numSeq1[k + 2], numSeq1[i]) + Sd5(i, k + 2) + EntropyDPT(k + 2, i)) && equal(HEND5(i), atPenaltyH(numSeq1[k + 2], numSeq1[i]) + Hd5(i, k + 2) + EnthalpyDPT(k + 2, i))) { push(&stack, k + 2, i, 0, o); break; } else if (equal(SEND5(i), SEND5(k) + atPenaltyS(numSeq1[k + 2], numSeq1[i]) + Sd5(i, k + 2) + EntropyDPT(k + 2, i)) && equal(HEND5(i), HEND5(k) + atPenaltyH(numSeq1[k + 2], numSeq1[i]) + Hd5(i, k + 2) + EnthalpyDPT(k + 2, i))) { push(&stack, k + 2, i, 0, o); push(&stack, k, 0, 1, o); break; } } else if (equal(SEND5(i), END5_3(i,2)) && equal(HEND5(i), END5_3(i,1))) { for (k = 0; k <= i - MIN_HRPN_LOOP - 3; ++k) if (equal(SEND5(i), atPenaltyS(numSeq1[k + 1], numSeq1[i - 1]) + Sd3(i - 1, k + 1) + EntropyDPT(k + 1, i - 1)) && equal(HEND5(i), atPenaltyH(numSeq1[k + 1], numSeq1[i - 1]) + Hd3(i - 1, k + 1) + EnthalpyDPT(k + 1, i - 1))) { push(&stack, k + 1, i - 1, 0, o); break; } else if (equal(SEND5(i), SEND5(k) + atPenaltyS(numSeq1[k + 1], numSeq1[i - 1]) + Sd3(i - 1, k + 1) + EntropyDPT(k + 1, i - 1)) && equal(HEND5(i), HEND5(k) + atPenaltyH(numSeq1[k + 1], numSeq1[i - 1]) + Hd3(i - 1, k + 1) + EnthalpyDPT(k + 1, i - 1))) { push(&stack, k + 1, i - 1, 0, o); /* matrix 0 */ push(&stack, k, 0, 1, o); /* matrix 3 */ break; } } else if(equal(SEND5(i), END5_4(i,2)) && equal(HEND5(i), END5_4(i,1))) { for (k = 0; k <= i - MIN_HRPN_LOOP - 4; ++k) if (equal(SEND5(i), atPenaltyS(numSeq1[k + 2], numSeq1[i - 1]) + Ststack(i - 1, k + 2) + EntropyDPT(k + 2, i - 1)) && equal(HEND5(i), atPenaltyH(numSeq1[k + 2], numSeq1[i - 1]) + Htstack(i - 1, k + 2) + EnthalpyDPT(k + 2, i - 1))) { push(&stack, k + 2, i - 1, 0, o); break; } else if (equal(SEND5(i), SEND5(k) + atPenaltyS(numSeq1[k + 2], numSeq1[i - 1]) + Ststack(i - 1, k + 2) + EntropyDPT(k + 2, i - 1)) && equal(HEND5(i), HEND5(k) + atPenaltyH(numSeq1[k + 2], numSeq1[i - 1]) + Htstack(i - 1, k + 2) + EnthalpyDPT(k + 2, i - 1)) ) { push(&stack, k + 2, i - 1, 0, o); push(&stack, k, 0, 1, o); break; } } } else if(top->mtrx==0) { bp[i - 1] = j; bp[j - 1] = i; SH1[0] = -1.0; SH1[1] = _INFINITY; calc_hairpin(i, j, SH1, 1); /* 1 means that we use this method in traceback */ SH2[0] = -1.0; SH2[1] = _INFINITY; CBI(i,j,SH2,2,maxLoop); if (equal(EntropyDPT(i, j), Ss(i, j, 2) + EntropyDPT(i + 1, j - 1)) && equal(EnthalpyDPT(i, j), Hs(i, j, 2) + EnthalpyDPT(i + 1, j - 1))) { push(&stack, i + 1, j - 1, 0, o); } else if (equal(EntropyDPT(i, j), SH1[0]) && equal(EnthalpyDPT(i,j), SH1[1])); else if (equal(EntropyDPT(i, j), SH2[0]) && equal(EnthalpyDPT(i, j), SH2[1])) { int d, done; for (done = 0, d = j - i - 3; d >= MIN_HRPN_LOOP + 1 && d >= j - i - 2 - maxLoop && !done; --d) for (ii = i + 1; ii < j - d; ++ii) { jj = d + ii; EntropyEnthalpy[0] = -1.0; EntropyEnthalpy[1] = _INFINITY; calc_bulge_internal2(i, j, ii, jj,EntropyEnthalpy,1,maxLoop); if (equal(EntropyDPT(i, j), EntropyEnthalpy[0] + EntropyDPT(ii, jj)) && equal(EnthalpyDPT(i, j), EntropyEnthalpy[1] + EnthalpyDPT(ii, jj))) { push(&stack, ii, jj, 0, o); ++done; break; } } } else { } } free(top); } free(SH1); free(SH2); free(EntropyEnthalpy); } static void traceback(int i, int j, double RT, int* ps1, int* ps2, int maxLoop, thal_results* o) { int d, ii, jj, done; double* SH; SH = (double*) safe_malloc(2 * sizeof(double), o); ps1[i - 1] = j; ps2[j - 1] = i; while(1) { SH[0] = -1.0; SH[1] = _INFINITY; LSH(i,j,SH); if(equal(EntropyDPT(i,j),SH[0]) && equal(EnthalpyDPT(i,j),SH[1])) { break; } done = 0; if (i > 1 && j > 1 && equal(EntropyDPT(i,j), Ss(i - 1, j - 1, 1) + EntropyDPT(i - 1, j - 1))) { i = i - 1; j = j - 1; ps1[i - 1] = j; ps2[j - 1] = i; done = 1; } for (d = 3; !done && d <= maxLoop + 2; ++d) { ii = i - 1; jj = -ii - d + (j + i); if (jj < 1) { ii -= abs(jj-1); jj = 1; } for (; !done && ii > 0 && jj < j; --ii, ++jj) { SH[0] = -1.0; SH[1] = _INFINITY; calc_bulge_internal(ii, jj, i, j, SH,1,maxLoop); if (equal(EntropyDPT(i, j), SH[0]) && equal(EnthalpyDPT(i, j), SH[1])) { i = ii; j = jj; ps1[i - 1] = j; ps2[j - 1] = i; done = 1; break; } } } } free(SH); } static void drawDimer(int* ps1, int* ps2, double temp, double H, double S, int temponly, double t37, thal_results *o) { int i, j, k, numSS1, numSS2, N; char* duplex[4]; double G, t; t = G = 0; if (!isFinite(temp)){ if(temponly==0) { printf("No predicted secondary structures for given sequences\n"); } o->temp = 0.0; /* lets use generalization here; this should rather be very negative value */ strcpy(o->msg, "No predicted sec struc for given seq"); return; } else { N=0; for(i=0;i0) ++N; } for(i=0;i0) ++N; } N = (N/2) -1; t = ((H) / (S + (N * saltCorrection) + RC)) - ABSOLUTE_ZERO; if(temponly==0) { G = (H) - (t37 * (S + (N * saltCorrection))); S = S + (N * saltCorrection); o->temp = (double) t; /* maybe user does not need as precise as that */ /* printf("Thermodynamical values:\t%d\tdS = %g\tdH = %g\tdG = %g\tt = %g\tN = %d, SaltC=%f, RC=%f\n", len1, (double) S, (double) H, (double) G, (double) t, (int) N, saltCorrection, RC); */ printf("Calculated thermodynamical parameters for dimer:\tdS = %g\tdH = %g\tdG = %g\tt = %g\n", (double) S, (double) H, (double) G, (double) t); } else { o->temp = (double) t; return; } } duplex[0] = (char*) safe_malloc(len1 + len2 + 1, o); duplex[1] = (char*) safe_malloc(len1 + len2 + 1, o); duplex[2] = (char*) safe_malloc(len1 + len2 + 1, o); duplex[3] = (char*) safe_malloc(len1 + len2 + 1, o); duplex[0][0] = duplex[1][0] = duplex[2][0] = duplex[3][0] = 0; i = 0; numSS1 = 0; while (ps1[i++] == 0) ++numSS1; j = 0; numSS2 = 0; while (ps2[j++] == 0) ++numSS2; if (numSS1 >= numSS2){ for (i = 0; i < numSS1; ++i) { strcatc(duplex[0], oligo1[i]); strcatc(duplex[1], ' '); strcatc(duplex[2], ' '); } for (j = 0; j < numSS1 - numSS2; ++j) strcatc(duplex[3], ' '); for (j = 0; j < numSS2; ++j) strcatc(duplex[3], oligo2[j]); } else { for (j = 0; j < numSS2; ++j) { strcatc(duplex[3], oligo2[j]); strcatc(duplex[1], ' '); strcatc(duplex[2], ' '); } for (i = 0; i < numSS2 - numSS1; ++i) strcatc(duplex[0], ' '); for (i = 0; i < numSS1; ++i) strcatc(duplex[0], oligo1[i]); } i = numSS1 + 1; j = numSS2 + 1; while (i <= len1) { while (i <= len1 && ps1[i - 1] != 0 && j <= len2 && ps2[j - 1] != 0) { strcatc(duplex[0], ' '); strcatc(duplex[1], oligo1[i - 1]); strcatc(duplex[2], oligo2[j - 1]); strcatc(duplex[3], ' '); ++i; ++j; } numSS1 = 0; while (i <= len1 && ps1[i - 1] == 0) { strcatc(duplex[0], oligo1[i - 1]); strcatc(duplex[1], ' '); ++numSS1; ++i; } numSS2 = 0; while (j <= len2 && ps2[j - 1] == 0) { strcatc(duplex[2], ' '); strcatc(duplex[3], oligo2[j - 1]); ++numSS2; ++j; } if (numSS1 < numSS2) for (k = 0; k < numSS2 - numSS1; ++k) { strcatc(duplex[0], '-'); strcatc(duplex[1], ' '); } else if (numSS1 > numSS2) for (k = 0; k < numSS1 - numSS2; ++k) { strcatc(duplex[2], ' '); strcatc(duplex[3], '-'); } } printf("SEQ\t"); printf("%s\n", duplex[0]); printf("SEQ\t"); printf("%s\n", duplex[1]); printf("STR\t"); printf("%s\n", duplex[2]); printf("STR\t"); printf("%s\n", duplex[3]); free(duplex[0]); free(duplex[1]); free(duplex[2]); free(duplex[3]); return; } static void drawHairpin(int* bp, double mh, double ms, int temponly, double temp, thal_results *o) { /* Plain text */ int i, N; N = 0; double mg, t; if (!isFinite(ms) || !isFinite(mh)) { if(temponly == 0) { printf("0\tdS = %g\tdH = %g\tinf\tinf\n", (double) ms,(double) mh); #ifdef DEBUG fputs("No temperature could be calculated\n",stderr); #endif } else { o->temp = 0.0; /* lets use generalization here */ strcpy(o->msg, "No predicted sec struc for given seq\n"); } } else { if(temponly == 0) { for (i = 1; i < len1; ++i) { if(bp[i-1] > 0) N++; } } else { for (i = 1; i < len1; ++i) { if(bp[i-1] > 0) N++; } } t = (mh / (ms + (((N/2)-1) * saltCorrection))) - ABSOLUTE_ZERO; if(temponly == 0) { mg = mh - (temp * (ms + (((N/2)-1) * saltCorrection))); ms = ms + (((N/2)-1) * saltCorrection); o->temp = (double) t; printf("Calculated thermodynamical parameters for dimer:\t%d\tdS = %g\tdH = %g\tdG = %g\tt = %g\n", len1, (double) ms, (double) mh, (double) mg, (double) t); } else { o->temp = (double) t; return; } } /* plain-text output */ char* asciiRow; asciiRow = (char*) safe_malloc(len1, o); for(i = 0; i < len1; ++i) asciiRow[i] = '0'; for(i = 1; i < len1+1; ++i) { if(bp[i-1] == 0) { asciiRow[(i-1)] = '-'; } else { if(bp[i-1] > (i-1)) { asciiRow[(bp[i-1]-1)]='\\'; } else { asciiRow[(bp[i-1]-1)]='/'; } } } printf("SEQ\t"); for(i = 0; i < len1; ++i) printf("%c",asciiRow[i]); printf("\nSTR\t%s\n", oligo1); free(asciiRow); return; } static int equal(double a, double b) { #ifdef INTEGER return a == b; #endif if (!finite(a) || !finite(b)) return 0; return fabs(a - b) < 1e-5; if (a == 0 && b == 0) return 1; } static void strcatc(char* str, char c) { str[strlen(str) + 1] = 0; str[strlen(str)] = c; } primer3-2.3.6/src/oligotm.c0000664000077300007730000004357212231156245014476 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of the oligotm library. The oligotm library is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. The oligotm library is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with the oligtm library (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include #include #include #include "oligotm.h" #define T_KELVIN 273.15; #define A_CHAR 'A' #define G_CHAR 'G' #define T_CHAR 'T' #define C_CHAR 'C' #define N_CHAR 'N' #define CATID5(A,B,C,D,E) A##B##C##D##E #define CATID2(A,B) A##B #define DO_PAIR(LAST,THIS) \ if (CATID2(THIS,_CHAR) == c) { \ dh += CATID5(H,_,LAST,_,THIS); \ ds += CATID5(S,_,LAST,_,THIS); \ goto CATID2(THIS,_STATE); \ } #define STATE(LAST) \ CATID2(LAST,_STATE): \ c = *s; s++; \ DO_PAIR(LAST,A) \ else DO_PAIR(LAST,T) \ else DO_PAIR(LAST,G) \ else DO_PAIR(LAST,C) \ else DO_PAIR(LAST,N) \ else if ('\0' == c) \ goto DONE; \ else goto ERROR \ #define DO_PAIR2(LAST,THIS) \ if (CATID2(THIS,_CHAR) == c) { \ dh += CATID5(DH,_,LAST,_,THIS); \ ds += CATID5(DS,_,LAST,_,THIS); \ goto CATID2(THIS,_STATE2); \ } #define STATE2(LAST) \ CATID2(LAST,_STATE2): \ c = *s; s++; \ DO_PAIR2(LAST,A) \ else DO_PAIR2(LAST,T) \ else DO_PAIR2(LAST,G) \ else DO_PAIR2(LAST,C) \ else DO_PAIR2(LAST,N) \ else if ('\0' == c) \ goto DONE; \ else goto ERROR \ /* * Two tables of nearest-neighbor parameters for di-nucleotide * base pairs. * * These are included in this file because they are not needed by * clients (callers) of oligtm(). */ /* Table 1 (old parameters): * See table 2 in the paper [Breslauer KJ, Frank R, Blöcker H and * Marky LA (1986) "Predicting DNA duplex stability from the base * sequence" Proc Natl Acad Sci 83:4746-50 * http://dx.doi.org/10.1073/pnas.83.11.3746] */ #define S_A_A 240 #define S_A_C 173 #define S_A_G 208 #define S_A_T 239 #define S_A_N 215 #define S_C_A 129 #define S_C_C 266 #define S_C_G 278 #define S_C_T 208 #define S_C_N 220 #define S_G_A 135 #define S_G_C 267 #define S_G_G 266 #define S_G_T 173 #define S_G_N 210 #define S_T_A 169 #define S_T_C 135 #define S_T_G 129 #define S_T_T 240 #define S_T_N 168 #define S_N_A 168 #define S_N_C 210 #define S_N_G 220 #define S_N_T 215 #define S_N_N 203 #define H_A_A 91 #define H_A_C 65 #define H_A_G 78 #define H_A_T 86 #define H_A_N 80 #define H_C_A 58 #define H_C_C 110 #define H_C_G 119 #define H_C_T 78 #define H_C_N 91 #define H_G_A 56 #define H_G_C 111 #define H_G_G 110 #define H_G_T 65 #define H_G_N 85 #define H_T_A 60 #define H_T_C 56 #define H_T_G 58 #define H_T_T 91 #define H_T_N 66 #define H_N_A 66 #define H_N_C 85 #define H_N_G 91 #define H_N_T 80 #define H_N_N 80 /* Delta G's of disruption * 1000. */ #define G_A_A 1900 #define G_A_C 1300 #define G_A_G 1600 #define G_A_T 1500 #define G_A_N 1575 #define G_C_A 1900 #define G_C_C 3100 #define G_C_G 3600 #define G_C_T 1600 #define G_C_N 2550 #define G_G_A 1600 #define G_G_C 3100 #define G_G_G 3100 #define G_G_T 1300 #define G_G_N 2275 #define G_T_A 900 #define G_T_C 1600 #define G_T_G 1900 #define G_T_T 1900 #define G_T_N 1575 #define G_N_A 1575 #define G_N_C 2275 #define G_N_G 2550 #define G_N_T 1575 #define G_N_N 1994 /* Table 2, new parameters: * Tables of nearest-neighbor thermodynamics for DNA bases, from the * paper [SantaLucia JR (1998) "A unified view of polymer, dumbbell * and oligonucleotide DNA nearest-neighbor thermodynamics", Proc Natl * Acad Sci 95:1460-65 http://dx.doi.org/10.1073/pnas.95.4.1460] */ #define DS_A_A 222 #define DS_A_C 224 #define DS_A_G 210 #define DS_A_T 204 #define DS_A_N 224 #define DS_C_A 227 #define DS_C_C 199 #define DS_C_G 272 #define DS_C_T 210 #define DS_C_N 272 #define DS_G_A 222 #define DS_G_C 244 #define DS_G_G 199 #define DS_G_T 224 #define DS_G_N 244 #define DS_T_A 213 #define DS_T_C 222 #define DS_T_G 227 #define DS_T_T 222 #define DS_T_N 227 #define DS_N_A 168 #define DS_N_C 210 #define DS_N_G 220 #define DS_N_T 215 #define DS_N_N 220 #define DH_A_A 79 #define DH_A_C 84 #define DH_A_G 78 #define DH_A_T 72 #define DH_A_N 72 #define DH_C_A 85 #define DH_C_C 80 #define DH_C_G 106 #define DH_C_T 78 #define DH_C_N 78 #define DH_G_A 82 #define DH_G_C 98 #define DH_G_G 80 #define DH_G_T 84 #define DH_G_N 80 #define DH_T_A 72 #define DH_T_C 82 #define DH_T_G 85 #define DH_T_T 79 #define DH_T_N 72 #define DH_N_A 72 #define DH_N_C 80 #define DH_N_G 78 #define DH_N_T 72 #define DH_N_N 72 /* Delta G's of disruption * 1000. */ #define DG_A_A 1000 #define DG_A_C 1440 #define DG_A_G 1280 #define DG_A_T 880 #define DG_A_N 880 #define DG_C_A 1450 #define DG_C_C 1840 #define DG_C_G 2170 #define DG_C_T 1280 #define DG_C_N 1450 #define DG_G_A 1300 #define DG_G_C 2240 #define DG_G_G 1840 #define DG_G_T 1440 #define DG_G_N 1300 #define DG_T_A 580 #define DG_T_C 1300 #define DG_T_G 1450 #define DG_T_T 1000 #define DG_T_N 580 #define DG_N_A 580 #define DG_N_C 1300 #define DG_N_G 1280 #define DG_N_T 880 #define DG_N_N 580 /* End of tables nearest-neighbor parameter. */ /* Calculate the melting temperature of oligo s. See oligotm.h for documentation of arguments. */ double oligotm(const char *s, double DNA_nM, double K_mM, double divalent_conc, double dntp_conc, tm_method_type tm_method, salt_correction_type salt_corrections) { register int dh = 0, ds = 0; register char c; double delta_H, delta_S; double Tm; /* Melting temperature */ double correction; int len, sym; const char* d = s; if(divalent_to_monovalent(divalent_conc, dntp_conc) == OLIGOTM_ERROR) return OLIGOTM_ERROR; /** K_mM = K_mM + divalent_to_monovalent(divalent_conc, dntp_conc); **/ if (tm_method != breslauer_auto && tm_method != santalucia_auto) return OLIGOTM_ERROR; if (salt_corrections != schildkraut && salt_corrections != santalucia && salt_corrections != owczarzy) return OLIGOTM_ERROR; len = (strlen(s)-1); sym = symmetry(s); /*Add symmetry correction if seq is symmetrical*/ if( tm_method == breslauer_auto ) { ds=108; } else { if(sym == 1) { ds+=14; } /** Terminal AT penalty **/ if(strncmp("A", s, 1)==0 || strncmp("T", s, 1)==0) { ds += -41; dh += -23; } else if (strncmp("C", s, 1)==0 || strncmp("G", s, 1)==0) { ds += 28; dh += -1; } s+=len; if(strncmp("T", s, 1)==0 || strncmp("A", s, 1)==0) { ds += -41; dh += -23; } else if (strncmp("C", s, 1)==0 || strncmp("G", s, 1)==0) { ds += 28; dh += -1; } s-=len; } /* Use a finite-state machine (DFA) to calucluate dh and ds for s. */ c = *s; s++; if (tm_method == breslauer_auto) { if (c == 'A') goto A_STATE; else if (c == 'G') goto G_STATE; else if (c == 'T') goto T_STATE; else if (c == 'C') goto C_STATE; else if (c == 'N') goto N_STATE; else goto ERROR; STATE(A); STATE(T); STATE(G); STATE(C); STATE(N); } else { if (c == 'A') goto A_STATE2; else if (c == 'G') goto G_STATE2; else if (c == 'T') goto T_STATE2; else if (c == 'C') goto C_STATE2; else if (c == 'N') goto N_STATE2; else goto ERROR; STATE2(A); STATE2(T); STATE2(G); STATE2(C); STATE2(N); } DONE: /* dh and ds are now computed for the given sequence. */ delta_H = dh * -100.0; /* * Nearest-neighbor thermodynamic values for dh * are given in 100 cal/mol of interaction. */ delta_S = ds * -0.1; /* * Nearest-neighbor thermodynamic values for ds * are in in .1 cal/K per mol of interaction. */ Tm=0; /* Melting temperature */ len=len+1; /**********************************************/ if (salt_corrections == schildkraut) { K_mM = K_mM + divalent_to_monovalent(divalent_conc, dntp_conc); correction = 16.6 * log10(K_mM/1000.0) - T_KELVIN; Tm = delta_H / (delta_S + 1.987 * log(DNA_nM/4000000000.0)) + correction; } else if (salt_corrections== santalucia) { K_mM = K_mM + divalent_to_monovalent(divalent_conc, dntp_conc); delta_S = delta_S + 0.368 * (len - 1) * log(K_mM / 1000.0 ); if(sym == 1) { /* primer is symmetrical */ /* Equation A */ Tm = delta_H / (delta_S + 1.987 * log(DNA_nM/1000000000.0)) - T_KELVIN; } else { /* Equation B */ Tm = delta_H / (delta_S + 1.987 * log(DNA_nM/4000000000.0)) - T_KELVIN; } } else if (salt_corrections == owczarzy) { double gcPercent=0; double free_divalent; /* conc of divalent cations minus dNTP conc */ int i; for(i = 0; i <= len && d != NULL && d != '\0';) { if(*d == 'C' || *d == 'G') { gcPercent++; } d++; i++; } gcPercent = (double)gcPercent/((double)len); /**** BEGIN: UPDATED SALT BY OWCZARZY *****/ /* different salt corrections for monovalent (Owczarzy et al.,2004) and divalent cations (Owczarzy et al.,2008) */ /* competition bw magnesium and monovalent cations, see Owczarzy et al., 2008 Figure 9 and Equation 16 */ static const double crossover_point = 0.22; /* depending on the value of div_monov_ratio respect to value of crossover_point Eq 16 (divalent corr, Owczarzy et al., 2008) or Eq 22 (monovalent corr, Owczarzy et al., 2004) should be used */ double div_monov_ratio; if(dntp_conc >= divalent_conc) { free_divalent = 0.00000000001; /* to not to get log(0) */ } else { free_divalent = (divalent_conc - dntp_conc)/1000.0; } static double a = 0,b = 0,c = 0,d = 0,e = 0,f = 0,g = 0; if(K_mM==0) { div_monov_ratio = 6.0; } else { div_monov_ratio = (sqrt(free_divalent))/(K_mM/1000); /* if conc of monov cations is provided a ratio is calculated to further calculate the _correct_ correction */ } if (div_monov_ratio < crossover_point) { /* use only monovalent salt correction, Eq 22 (Owczarzy et al., 2004) */ correction = (((4.29 * gcPercent) - 3.95) * pow(10,-5) * log(K_mM / 1000.0)) + (9.40 * pow(10,-6) * (pow(log(K_mM / 1000.0),2))); } else { /* magnesium effects are dominant, Eq 16 (Owczarzy et al., 2008) is used */ b =- 9.11 * pow(10,-6); c = 6.26 * pow(10,-5); e =- 4.82 * pow(10,-4); f = 5.25 * pow(10,-4); a = 3.92 * pow(10,-5); d = 1.42 * pow(10,-5); g = 8.31 * pow(10,-5); if(div_monov_ratio < 6.0) { /* in particular ratio of conc of monov and div cations * some parameters of Eq 16 must be corrected (a,d,g) */ a = 3.92 * pow(10,-5) * (0.843 - (0.352 * sqrt(K_mM/1000.0) * log(K_mM/1000.0))); d = 1.42 * pow(10,-5) * (1.279 - 4.03 * pow(10,-3) * log(K_mM/1000.0) - 8.03 * pow(10,-3) * pow(log(K_mM/1000.0),2)); g = 8.31 * pow(10,-5) * (0.486 - 0.258 * log(K_mM/1000.0) + 5.25 * pow(10,-3) * pow(log(K_mM/1000.0),3)); } correction = a + (b * log(free_divalent)) + gcPercent * (c + (d * log(free_divalent))) + (1/(2 * (len - 1))) * (e + (f * log(free_divalent)) + g * (pow((log(free_divalent)),2))); } /**** END: UPDATED SALT BY OWCZARZY *****/ if (sym == 1) { /* primer is symmetrical */ /* Equation A */ Tm = 1/((1/(delta_H / (delta_S + 1.9872 * log(DNA_nM/1000000000.0)))) + correction) - T_KELVIN; } else { /* Equation B */ Tm = 1/((1/(delta_H / (delta_S + 1.9872 * log(DNA_nM/4000000000.0)))) + correction) - T_KELVIN; } } /* END else if (salt_corrections == owczarzy) { */ /***************************************/ return Tm; ERROR: /* * length of s was less than 2 or there was an illegal character in * s. */ return OLIGOTM_ERROR; } #undef DO_PAIR #undef DO_PAIR2 #define DO_PAIR(LAST,THIS) \ if (CATID2(THIS,_CHAR) == c) { \ dg += CATID5(G,_,LAST,_,THIS); \ goto CATID2(THIS,_STATE); \ } #define DO_PAIR2(LAST,THIS) \ if (CATID2(THIS,_CHAR) == c) { \ dg += CATID5(DG,_,LAST,_,THIS); \ goto CATID2(THIS,_STATE2); \ } double oligodg(const char *s, /* The sequence. */ int tm_method) { register int dg = 0; register char c; if (tm_method != breslauer_auto && tm_method != santalucia_auto) return OLIGOTM_ERROR; /* Use a finite-state machine (DFA) to calucluate dg s. */ c = *s; s++; if(tm_method != breslauer_auto) { dg=-1960; /* Initial dG */ if(c == 'A' || c == 'T') { dg += -50; /* terminal AT penalty */ } if (c == 'A') goto A_STATE2; else if (c == 'G') goto G_STATE2; else if (c == 'T') goto T_STATE2; else if (c == 'C') goto C_STATE2; else if (c == 'N') goto N_STATE2; else goto ERROR; STATE2(A); STATE2(T); STATE2(G); STATE2(C); STATE2(N); } else { if (c == 'A') goto A_STATE; else if (c == 'G') goto G_STATE; else if (c == 'T') goto T_STATE; else if (c == 'C') goto C_STATE; else if (c == 'N') goto N_STATE; else goto ERROR; STATE(A); STATE(T); STATE(G); STATE(C); STATE(N); } DONE: /* dg is now computed for the given sequence. */ if(tm_method != breslauer_auto) { int sym; --s; --s; c = *s; if(c == 'A' || c == 'T') { dg += -50; /* terminal AT penalty */ } sym = symmetry(s); if(sym==1) { dg +=-430; /* symmetry correction for dG */ } } return dg / 1000.0; ERROR: /* * length of s was less than 2 or there was an illegal character in * s. */ return OLIGOTM_ERROR; } double end_oligodg(const char *s, int len, /* The number of characters to return. */ int tm_method) { int x = strlen(s); if (tm_method != breslauer_auto && tm_method != santalucia_auto) return OLIGOTM_ERROR; return x < len ? oligodg(s,tm_method) : oligodg(s + (x - len),tm_method); } /* See oligotm.h for documentation of arguments. */ double seqtm(const char *seq, double dna_conc, double salt_conc, double divalent_conc, double dntp_conc, int nn_max_len, tm_method_type tm_method, salt_correction_type salt_corrections) { int len = strlen(seq); if (tm_method != breslauer_auto && tm_method != santalucia_auto) return OLIGOTM_ERROR; if (salt_corrections != schildkraut && salt_corrections != santalucia && salt_corrections != owczarzy) return OLIGOTM_ERROR; if (len > nn_max_len) { return long_seq_tm(seq, 0, len, salt_conc, divalent_conc, dntp_conc); } else { return oligotm(seq, dna_conc, salt_conc, divalent_conc, dntp_conc, tm_method, salt_corrections); } } /* See oligotm.h for documentation on this function and the formula it uses. */ double long_seq_tm(const char *s, int start, int len, double salt_conc, double divalent_conc, double dntp_conc) { int GC_count = 0; const char *p, *end; if (divalent_to_monovalent(divalent_conc, dntp_conc) == OLIGOTM_ERROR) return OLIGOTM_ERROR; salt_conc = salt_conc + divalent_to_monovalent(divalent_conc, dntp_conc); if ((unsigned) (start + len) > strlen(s) || start < 0 || len <= 0) return OLIGOTM_ERROR; end = &s[start + len]; /* Length <= 0 is nonsensical. */ for (p = &s[start]; p < end; p++) { if ('G' == *p || 'C' == *p) GC_count++; } return 81.5 + (16.6 * log10(salt_conc / 1000.0)) + (41.0 * (((double) GC_count) / len)) - (600.0 / len); } /* Return 1 if string is symmetrical, 0 otherwise. */ int symmetry(const char* seq) { register char s; register char e; const char *seq_end=seq; int i = 0; int seq_len=strlen(seq); int mp = seq_len/2; if(seq_len%2==1) { return 0; } seq_end+=seq_len; seq_end--; while(iproduct_size = 0 then primer choice failed. * Both functions may exit on error, using the conventions * in primer3_boulder_main.c */ void print_format_output(FILE *f, const int *io_version, const p3_global_settings *pa, const seq_args *sa, const p3retval *, const char *, int explain_flag); void format_error(FILE *f, const char* seq_name, const char *err); void format_warning(FILE *f, const char* seq_name, const char *err); #endif primer3-2.3.6/src/paranoid_tests.sh0000664000077300007730000000054212231156245016221 0ustar ioanaioanaexport PATH=/usr/bin:$PATH echo; echo; echo 'STARTING 1ST SET OF TESTS (-O2)'; echo; echo; make clean make test make clean echo; echo; echo 'STARTING 2ND SET OF TESTS (-O3)'; echo; echo; make O_OPTS=-O3 make test make clean echo; echo; echo 'STARTING 3RD SET OF TESTS (-O0 and valgrind)'; echo; echo; make O_OPTS=-O0 cd ../test make TESTOPTS=--valgrind primer3-2.3.6/src/release_notes.txt0000664000077300007730000006003412231156245016241 0ustar ioanaioanarelease 2.3.6 2013-10-22 =============================================== Important changes: 1. Four new input tags were added: - PRIMER_MUST_MATCH_THREE_PRIME - PRIMER_MUST_MATCH_FIVE_PRIME - PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME - PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME The MUST_MATCH tags allow to define 5 nt patterns which the first and the last five bases of a primer or oligo have to match. For example, the pattern "NNGTN" would require the 3rd and 2nd last bases to be G and T respectively. Minor fixes: 1. Fixed two bugs in ntdpal and oligotm that caused segmentation fault whenever these binaries were called without specifying a given option value. release 2.3.5 2013-01-03 =============================================== Important changes: 1. The tag PRIMER_THERMODYNAMIC_ALIGNMENT was replaced by two new tags: - PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT which governs hairpin and oligo-oligo interactions. Therefore, whenever it is set to 1 (the default) thermodynamic alignments will be used for oligo- oligo interactions and hairpins. - PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT which governs the oligo-template interactions and, when set to 1 (default is 0), will cause primer3 to use the thermodynamic alignment against templates. The reason for this change is to provide the option of using the old alignment for oligo-template interactions when the thermodynamic alignment is too slow or the template is too long (there is a hard limit THAL_MAX_SEQ=10k on the length of sequences involved in thermodynamic alignments). 1.1 Added corresponding functions to libprimer3.c and libprimer3.h: p3_set_gs_primer_thermodynamic_oligo_alignment p3_set_gs_primer_thermodynamic_template_alignment 2. Modified the error handling of non-memory related errors that can occur during the thermodynamic alignment. They are no longer fatal errors. 3. Made a performance improvement for the case when thermodynamic alignment is used for templates: the alignment is done only once for each oligo and the result is kept. This way we avoid performing the same expensive alignment more than once. 4. Removed io_version 3, which was only kept for backward compatibility with very old versions of primer3. 5. Fixed an error in which PRIMER_DNTP_CONC > PRIMER_SALT_DIVALENT causes Primer3 to crash. 6. Fixed a bug that made Primer3 not generate results in some cases when PRIMER_FIRST_BASE_INDEX is negative. Minor corrections: 1. Added function p3_set_max_end_gc (sets max_end_gc in the primer_global_settings struct). 2. Removed intentional compile time division by zero in thal.c if macro INFINITY is defined. 3. Updated text in error messages from function thal() to make them more informative. release 2.3.4 2012-05-30 =============================================== Major correction: 1. Fixed error in which PRIMER_..._..._TEMPLATE_MISPRIMING_TH was calculated incorrectly in libprimer3.c release 2.3.3 2012-05-18 =============================================== Major correction: 1. Fixed error in which non-default values of monovalent and divalent salt concentrations and non-default values of dNTP and DNA concentrations were being ignored in thermodynamic calculations. release 2.3.2 2012-03-15 =============================================== Minor corrections: 1. _Removed_ warning if PRIMER_INTERNAL_SALT_DIVALENT > 0.0 but PRIMER_INTERNAL_DNTP_CONC <= 0.0. (dNTPs not used in hybridizations.) release 2.3.1 2012-02-27 =============================================== Minor corrections: 1. Added warnings if PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0 or PRIMER_INTERNAL_SALT_DIVALENT > 0.0 but PRIMER_INTERNAL_DNTP_CONC <= 0.0. 2. No longer an error if a settings file has PRIMER_THERMODYNAMIC_ALIGNMENT=1 but does not have a valid PRIMER_THERMODYNAMIC_PARAMETERS_PATH. (PRIMER_THERMODYNAMIC_PARAMETERS_PATH can then be set in the regular boulder IO input stream.) 3. Updates to documetation on salt corrections. 4. Corrections to primer3web_v3_0_0_default_settings.txt (removed non-existent tags and removed the setting of PRIMER_THERMODYNAMIC_PARAMETERS_PATH) 5. Removed un-needed files from distribution .tar.gz file. 6. Removed the -i option from the oligotm executable, as the necessary information is in the Primer3 manual. 7. Updated PRIMER_PRODUCT_SIZE_RANGE in primer3web_v3_0_0_default_settings to same value as in previous version of primer3web. release 2.3.0 2012-02-03 =============================================== 1. *** We changed the following default values: 1.1 Changing default oligo temperature calculations PRIMER_TM_FORMULA=1 (was 0) PRIMER_SALT_CORRECTIONS=1 (was 0) 1.2 Making thermodynamic secondary structure calculations the default: PRIMER_THERMODYNAMIC_ALIGNMENT=1 (was 0) 1.3 The following need reasonable values to support the changes in 1.1 and 1.2 above: PRIMER_SALT_DIVALENT=1.5 (was 0.0) PRIMER_DNTP_CONC=0.6 (was 0.0) 1.4 To make old defaults easily accessible, we added a command line argument, --default_version=1, which directs primer3_core to use the old defaults. --default_version=2 directs primer3_core to use the new defaults. The default is --default_version=2. 1.5 IMPORTANT: because PRIMER_THERMODYNAMIC_ALIGNMENT=1, PRIMER_THERMODYNAMIC_PARAMETERS_PATH must point to the right location. This tag specifies the path to the directory that contains all the parameter files used by the thermodynamic approach. In Linux, there are two *default* locations that are tested if this tag is not defined: ./primer3_config/ and /opt/primer3_config/. For Windows, there is only one default location: .\primer3_config\. If the the parameter files are not in one these locations, be sure to set PRIMER_THERMODYNAMIC_PARAMETERS_PATH 1.6 Changed default for PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 (was 1) (0 is almost always the the behavior one wants.) 1.7 To get the behavior of -default_version=1 when -default_version=2 set the following: PRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=0 PRIMER_THERMODYNAMIC_ALIGNMENT=0 PRIMER_SALT_DIVALENT=0.0 PRIMER_DNTP_CONC=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 1.8 To get the behavior of -default_version=2 when -default_version=1 set the following: PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_THERMODYNAMIC_ALIGNMENT=1 PRIMER_SALT_DIVALENT=1.5 PRIMER_DNTP_CONC=0.6 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 2. We changed the NULL value for SEQUENCE_FORCE_{LEFT,RIGHT}_START_END to -1000000, and made it an error to select PRIMER_FIRST_BASE_INDEX <= this value. This is to correct an error when SEQUENCE_FORCE_{LEFT,RIGHT}_START_END was -1 (value previously used to indicate a NULL) but PRIMER_FIRST_BASE_INDEX was < 0, which caused the intended NULL value (-1) to be treated as a constraint on primer location (a constraint that was not possible to satisfy). 3. We changed the PRIMER_TASK called 'pick_detection_primers' to 'generic' while retaining 'pick_detection_primers' as an alias for backward compatibility. 4. The code now uses 'end' alignments when assessing template mispriming using thermodynamic alignments. This is consistent with the approach taken with the previous alignment algorithm and with checking for mispriming against repeat libraries. 4. We removed PRIMER_PAIR_MAX_HAIRPIN_TH (which was ignored previously). 5. Primer3 now requires the user to set SEQUENCE_TARGET, not SEQUENCE_INCLUDED_REGION when PRIMER_TASK=pick_discriminative_primers 5. When PRIMER_TASK=pick_discriminative_primers or PRIMER_TASK=pick_cloning_primers the value of SEQUENCE_INCLUDED_REGION is no longer changed to the entire input sequence. 6. The handling of divalent cations when PRIMER_SALT_CORRECTIONS=2 (not the default and not the recommended value) has been updated. The rationale is that, when divalent cations are present, the formula by Owczarzy et al., 2004 (used previously by Primer3) can be improved upon as described in (Ahsen et al., 2010; Owczarzy et al., 2008). Therefore we have updated the melting temperature calculation to follow the scheme in Figure 9 of (Owczarzy et al., 2008). Please find references to these papers in the Primer3 manual. 7. In addition, several error corrections: 7.1 Corrected a short, fixed-size buffer for the file/path names specified by the command line arguments -output, -error and for the settings file. 7.2 Two other corrected errors were in p3_set_gs_primer_self_end and p3_set_gs_primer_internal_oligo_self_end, which erroneously multiplied their 'val' arguments by 100. 7.3 Primer3 now detects and handles the situation in which user-supplied primers (SEQUENCE_PRIMER and SEQUENCE_PRIMER_REVCOMP) have the left primer to the right of the right primer. Also issue a warning if user-supplied primers occur in more than one location in the template. release 2.2.3 2010-07-08 =============================================== Minor error corrections, minor cleanup. This is a stable release. release 2.2.2 beta 2010-03-04 ========================================== This release adds a performance enhancement for inputs involving SEQUENCE_PRIMER_PAIR_OK_REGION_LIST. This release adds two new tags, PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE and PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE release 2.2.1 alpha 2009-10-28 ========================================== This release adds several performance enhancements. release 2.2.0 alpha 2009-10-23 ========================================== This release adds multiple new features to primer3_core and related C code. The enhancements fall under several categories: 1. PRIMER/OLIGO SECONDARY STRUCTURE AND PRIMER-DIMER AND TEMPLATE MISPRIMING PREDICTION We added capabilities for predicting secondary structure, primer-dimer, and template mispriming based on a thermodynamic model. INPUT/OUTPUT TAGS: Added tags PRIMER_THERMODYNAMIC_ALIGNMENT, PRIMER_INTERNAL_MAX_{HAIRPIN,SELF_ANY_TH,SELF_END_TH,TEMPLATE_MISHYB_TH}, PRIMER_INTERNAL_WT_{HAIRPIN,SELF_ANY_TH,SELF_END_TH,TEMPLATE_MISHYB_TH}, PRIMER_MAX_{HAIRPIN,SELF_ANY_TH,SELF_END_TH,TEMPLATE_MISPRIMING_TH}, PRIMER_PAIR_MAX_{COMPL_ANY_TH,COMPL_END_TH,HAIRPIN,TEMPLATE_MISPRIMING_TH}, PRIMER_PAIR_WT_{COMPL_ANY_TH,COMPL_END_TH,HAIRPIN,TEMPLATE_MISPRIMING_TH}, PRIMER_WT_{HAIRPIN,SELF_ANY_TH,SELF_END_TH,TEMPLATE_MISPRIMING_TH} See the documentation for details. The tag PRIMER_THERMODYNAMICAL_ALIGNMENT=1 directs primer3_core to use the thermodynamic alignments. NEW EXECUTABLE: ntthal ntthal is analogous to ntdpal. Between two sequences, ntthal finds alignment/sec structure, that has the highest melting temperature. Ntthal is based on nearest-neighbor thermodynamical approach. For input parameters to ntthal, execute: ./ntthal (without arguments). 2. NEW COMMAND LINE FLAGS AND ARGUMENTS primer3_core now has a more conventional command line. For example, you can type primer3_core -output my_output -error my_error my_input and primer3_core will take its input from my_input and put its outputs in the files my_output and my_error. There is also a command line flag -echo_settings_file to cause the settings file contents to be echoed to output. 3. NEW INPUT TAGS TO CONTROL PRIMER LOCATION See the documentation for SEQUENCE_PRIMER_PAIR_OK_REGION_LIST. Tags SEQUENCE_OVERLAP_JUNCTION_LIST, PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION, PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION have _replaced_ SEQUENCE_PRIMER_OVERLAP_POS and PRIMER_POS_OVERLAP_TO_END_DIST. See the documentation. 4. PERFORMANCE IMPROVEMENTS release 2.0.0 alpha 2008-12-15 ========================================== This is major release, involving enhancements to functionality, software architecture, and input and output conventions. INPUT/OUTPUT CHANGES: * A new command line flag, -io_version=3 provides backward compatible behavior for boulder IO input, and boulder IO output that is backward compatible except for error messages, warnings, and the text of PRIMER...EXPLAIN tags. * -io_version=3 IS NOT the default -- you must specify it on the command line. For the new default IO, we renamed many Boulder IO INPUT/OUTPUT TAGS for clarification and easier integration into scripts NEW OR IMPROVED FUNCTIONALITY: Primer3 'settings' files were added. A settings file can be read in at program start up to set global parameters. Our intent is that parameter values suitable for particular primer design objectives can be easily stored and re-used. We added new "tasks" such as selection of primer lists, sequencing primers, or cloning primers, or the checking of primers or primer pairs in the absence of a target sequence. See tag PRIMER_TASK. It is now possible to request only unique primers when multiple primer pairs are requested. (In the past, each _pair_ was unique, but the same primer might occur in more than one pair). The user also has some control over what constitutes a 'unique' primer. See tag PRIMER_MIN_THREE_PRIME_DISTANCE. When an illegal primer pair is specified for checking primer3, the program now assess it. In the past, primer3 assessed individual primers if they violated a constraint, but in some cases not pairs that violated constraints on pairs (such as product size). We added new functionality with tags SEQUENCE_PRIMER_OVERLAP_POS, PRIMER_POS_OVERLAP_TO_END_DIST, PRIMER_MAX_END_GC. Please see the documentation. We added tags PRIMER_PAIR_MAX_COMPL_END and PRIMER_PAIR_MAX_COMPL_ANY, which previously took the same values as PRIMER_SELF_{END,ANY} OTHER CHANGES TO BEHAVIOR: We corrected a few errors and a mis-feature in the ways in which primer3 searches for primer pairs. Consequently, the latest version of primer3 will return different sets of primer pairs in a few instances. INTERNAL CHANGES: It is now possible to use the main primer3 functionality from a single C function. We cleanly separated the functionality of reading in Boulder IO records from the primer choosing / designing functionality. We also substantially simplified the organization and documentation of the main primer picking code, now in libprimer3.[ch]. The file libprimer3.h specifies the external interface, although it is not separately documented. It is also possible to create a binary library file that provides this functionality (libprimer3.a or libprimer3.so in Unix/Linux). All C code is ANSI C. release 1.1.2 2007-06-10 ================================================ Updates to dpal.c so that no error forces an exit. Adaptation in primer3_main.c and ntdpal_main.c to updated error handling in dpal(). Arranged for primer3_main.c align() to check for and circumvent local alignments to "targets" < nucleotides long, and added tests for this condition. Comment and code readability updates in dpal.h and dpal.c release 1.1.1 3/37/2007 ================================================== Minor corrections release 1.1.0 1/17/2007 ================================================== Added tags PRIMER_TM_SANTALUCIA, PRIMER_SALT_CORRECTIONS, PRIMER_LOWERCASE_MASKING, PRIMER_DIVALENT_CONC, and PRIMER_DNTP_CONC. See the README for details of what these tags do. Briefly, the first two tags allow the use of modern melting temperature calculations. New tests for this functionality were added to the tests/ subdirectory NOTE: The oligotm executable now takes additional arguments. Cleaned up Makefile, and created Makefile for the test directory with a target to clean up after tests Most (but not all) temporary files created by the tests now end in .tmp or .tmp2 Tried to create more informative test for MAX_PRIMER_LENGTH > DPAL_MAX_ALIGN in primer3_main.c NOTE: The oligtotm() function is now provided as a library, liboligotm. NOTE: non-backward compatible change: removed support of flag -2x_compat. release 1.0.1 11/06 ================================================== Identical to 1.0b except for release number release 1.0b 11/06 ================================================== Corrected bug involving check for template mispriming Minor code cleanup release 1.0a 9/06 ================================================== No new functionality (bug fixes and minor code cleanup only) Bug fixes: * Corrected calculation of ..._TEMPLATE_MISPRIMING when INCLUDED_REGION was set (thanks to Tomoaki Nishiyama, Advanced Sciences Research Center, Kanazawa University, Japan). * Corrected error that caused crash when PRIMER_WT_TEMPLATE_MISPRIMING was set (thanks to Peng Yu, Functional Genomics Group II, Chinese National Human Genome Center, Beijing). * Added olgiotm_main.c (for executable oligotm) release 1.0 6/04 ================================================== Added ability to search source sequence (template) for mispriming or mishybing sites. Added _experimental_ facility to calculate Tm on long oligos using a GC% based formula. Miscellaneous fixes and cleanup, including: * Avoiding closing NULL file handles when a mispriming or mishyb library is missing * Correction to long_seq_tm() in oligotm.c plus addition of related tests. * Gave user the ability to _not_ treat IUB/IUPAC ambiguity codes as a consensus while matching against mispriming or mishyb libraries. Inability to turn off this "feature" caused severe problems when libraries contained strings of N's: NNNNNNNNN, since if N is treated as a consensus, any base matches. release 0.9 9/98 ================================================== Corrected errors in calculation of amplicon Tm. release 0.8 6/98 ================================================== Added flexible objective function. Various tags governing the penalty weights are not fully documented in this release. Added much changed web interface. Added ability to select only a single left or right primer or hyb probe. Made it possible to continue even if PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_INPUT is not legal. Added PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_GC_PERCENT output tags. release 0.7 5/98 ================================================== Fixed the 'case problem' for PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_INPUT (and at the same time a related bug). Added experimental PRIMER_START_CODON_POSITION tag and related outputs. This feature should still be considered experimental and the user should scrutinize the output. Added PRIMER_PRODUCT_{MAX,MIN}_TM and related outputs. release 0.6 ================================================== Bug fixes (most introduced as part of 0.5 enhancements). release 0.5 ================================================== ** Non-Backward compatible change: Fixed inconsistency in output format; PRIMER_INTERNAL_n_OLIGO_SEQUENCE -> PRIMER_INTERNAL_OLIGO_n_SEQUENCE. The old format can be preserved by compiling with the precompiler symbol USE_OLD_FORMAT_MISTAKE defined. Added end-anchored local alignment option to dpal. Removed some un-needed dpal implementations. Added end-anchored local alignments for checking mispriming libraries. Added mispriming estimates for primer _pairs_ (see PRIMER_PAIR_MISPRIMING_LIBRARY), with related new output tags. Trivial change in maximum allowable library sequence weight. Added an experimental primer position component to the objective function (PRIMER_{IN,OUT}SIDE_PENALTY), with related new output tags. Added sequence quality scores as constraints in primer picking (PRIMER_MIN_{,END_}_QUALITY, PRIMER_QUALITY_RANGE_{MIN,MAX}) with related new output tags. Added more error reporting to primer3.cgi when primer3 exits with a non-0 termination status. Fixed bug when non-0, non-1 value was supplied for pick internal oligos. Added PRIMER_{LEFT,RIGHT,INTERNAL_OLIGO}_INPUT for checking or for designing around existing primers or internal oligos. Added error checking for duplicates of certain tags, such as SEQUENCE. Added PRIMER_MAX_END_STABILITY and related changes to output. release 0.4b ================================================== Corrected bug in parsing of INCLUDED_REGION tag. release 0.4 ================================================== Added optional check of oligos against "mispriming" or "mishyb" libraries with related changes to formated output and to the contents of primer list files. Modified search algorithm to compute complementarity/ similarity measures as late as possible. Got rid of quotes (") around error and warning strings. Fixed numerical overflow on input sequences longer than MAX_SHRT. (Thanks to Pete Young for finding this one.) release 0.3 ================================================== Changes to dpal to make it substantially faster on the maxgap=1 case for local and end-anchored complementarity measures. Removed restriction on maximum sequence length (by removing ftar, fexcl, fexcl_int, fnn, fn, fgc arrays, which necessitated internal changes to methods for calculating overlap with excluded regions and targets and to calculate spanning of targets. Modified 'primer list' format (clean up and hopefully got rid of spurious regression test discrepancy on some systems due to differences in rounding). Exit codes for some errors changed. Text of error messages for PRIMER_PRODUCT_SIZE -> PRIMER_PRODUCT_SIZE_RANGE. Some errors that should have been global (fatal) were not. These were fixed and error handling rationalized somewhat. An error in the display of the start position of targets, and excluded regions in formated output was corrected. An error in which overlapping product size ranges caused primer3 to return duplicate primer pairs was corrected. Made regression tests more complete. Made the maximum number of primer pairs to return a parameter (PRIMER_NUM_RETURN). Added optional n-based indexing in input and output (PRIMER_FIRST_BASE_INDEX=n). Has not been tested for n < 0. release 0.2b (10/11/96) ================================================== Added PRIMER_WARNING tag. Made it legal for excluded regions and targets to lie outside of included region. Documentation fixes (especially to PRIMER_DNA_CONC). release 0.2a ================================================== Tweaks to formated output to make it more complete (included complementarity measures); adjusted tests. release 0.2 ================================================== Made 0-length excluded region legal, and adjusted tests. Tweaked error reporting of negative-length excluded regions, internal oligo excluded regions, and target. ============================================================== ============================================================== Compared to non-primer3 progenitors: Compared to primer 0.5 --------------- Completely different input format. It has been reported the 0.5 deleted Ns when they occurred in primers. More stringent self-complementarity defaults. Primer3 selects internal oligos on request (and produces .int files if requested). Compared to both primer 0.5 and primer v2 --------------------------- The format of the contents of .for, .rev (and .int) files is different. Primer3 returns a user-specifiable number of primer pairs (or triples) sorted by "goodness". Primer3 will find a primer pair if any acceptable pair exists. Optional n-based indexing into source sequence. Use of sequence quality and 3' stability as constraints in primer picking. Optional positional component to objective function. Compared to primer v2 ------------- Tag name changes. However, primer3 should understand most or all Primer v2 input tags, and should produce v2-compatible output tag names when the -v2_compat command-line switch is used. The one exception is that the PRIMER_RECOMMEND tag is no longer produced. Instead primer3 produces the PRIMER_x_EXPLAIN output tags. The format of the data in this tags is different from the data in v2's PRIMER_RECOMMEND output tag. Numerous fixes. Uses the PRIMER_SELF_ANY and PRIMER_SELF_END parameters to govern maximum allowable complementarity between left and right primers, as well as complementarity between copies of a single oligo or within a single oligo. This behaviour is very close to that of primer 0.5; self complementarity calculations in v2 were unreliable. Primer3 produces much more output information, including the TMs and self complementarity measures of selected primers. primer3-2.3.6/src/libprimer3.c0000664000077300007730000107506312231156245015075 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009, 2010,2011,2012 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky. All rights reserved. This file is part of the primer3 suite and libraries. The primer3 suite and libraries are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this software (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include #include #include #include #include #include #include #include #include /* toupper */ #ifdef __GNUC__ #include #else #include #endif namespace std { using namespace __gnu_cxx; } #include "dpal.h" #include "thal.h" #include "oligotm.h" #include "libprimer3.h" /* #define's */ /* * OPTIMIZE_OK_REGIONS 1 allows _optimize_ok_regions_list() to use the * max/min product size info and the max/min oligo length to reduce * the sizes of the ok regions (while still generating the same primer * pairs in the same order). Set OPTIMIZE_OK_REGIONS to 0 confirm * that the results do not change. (The output tags * PRIMER_{LEFT,RIGHT,PAIR}_EXPLAIN _will_ likely change.) */ #define OPTIMIZE_OK_REGIONS 1 #ifndef MAX_PRIMER_LENGTH #define MAX_PRIMER_LENGTH 36 #endif #if (MAX_PRIMER_LENGTH > DPAL_MAX_ALIGN) #error "MAX_PRIMER_LENGTH must be <= DPAL_MAX_ALIGN" #endif #if (MAX_PRIMER_LENGTH > THAL_MAX_ALIGN) # error "MAX_PRIMER_LENGTH must be <= THAL_MAX_ALIGN" #endif #define MAX_NN_TM_LENGTH 36 /* The maxium length for which to use the nearest neighbor model when calculating oligo Tms. */ #define MACRO_CAT_2(A,B) A##B #define MACRO_VALUE_AS_STRING(A) MACRO_STRING(A) #define PR_POSITION_PENALTY_IS_NULL(PA) \ (PR_DEFAULT_INSIDE_PENALTY == (PA)->inside_penalty \ && PR_DEFAULT_OUTSIDE_PENALTY == (PA)->outside_penalty) #define INITIAL_LIST_LEN 2000 /* Initial size of oligo lists. */ #define INITIAL_NUM_RETURN 5 /* Initial space to allocate for pairs to return. */ #define PAIR_OK 1 #define PAIR_FAILED 0 #define OK_OR_MUST_USE(H) (!p3_ol_has_any_problem(H) || (H)->must_use) #define PR_UNDEFINED_INT_OPT INT_MIN #define PR_UNDEFINED_DBL_OPT DBL_MIN /* Undefined value for alignment score (meaning do not check) used for maximum template mispriming or mishyb. */ #define PR_UNDEFINED_ALIGN_OPT -100.0 #define TRIMMED_SEQ_LEN(X) ((X)->incl_l) typedef struct dpal_arg_holder { dpal_args *local; dpal_args *end; dpal_args *local_end; dpal_args *local_ambig; dpal_args *local_end_ambig; } dpal_arg_holder; typedef struct thal_arg_holder { thal_args *any; thal_args *end1; thal_args *end2; thal_args *hairpin_th; } thal_arg_holder; static jmp_buf _jmp_buf; static int thermodynamic_alignment_length_error = 0; static char *thermodynamic_alignment_length_error_msg = NULL; /* Variables needed in choose_pair_or_triple, need to be global for memory de-allocation reasons. Full description given in the function. */ static int *max_j_seen; static std::hash_map **pairs; /* Function declarations. */ static void pr_set_default_global_args_1(p3_global_settings *); static void pr_set_default_global_args_2(p3_global_settings *); static void _adjust_seq_args(const p3_global_settings *pa, seq_args *sa, pr_append_str *nonfatal_err, pr_append_str *warning); static void _optimize_ok_regions_list(const p3_global_settings *pa, seq_args *sa); static int any_5_prime_ol_extension_has_problem(const primer_rec *); static int p3_ol_is_uninitialized(const primer_rec *); static int fake_a_sequence(seq_args *sa, const p3_global_settings *pa); static int _pr_data_control(const p3_global_settings *, const seq_args *, pr_append_str *glob_err, pr_append_str *nonfatal_err, pr_append_str *warning); static int _pr_need_pair_template_mispriming(const p3_global_settings *pa); static int _pr_need_pair_template_mispriming_thermod(const p3_global_settings *pa); static int _pr_need_template_mispriming(const p3_global_settings *); static int _pr_need_template_mispriming_thermod(const p3_global_settings *); static void _pr_substr(const char *, int, int, char *); static int _check_and_adjust_intervals(seq_args *sa, int seq_len, int first_index, pr_append_str * nonfatal_err, pr_append_str *warning); static int _check_and_adjust_overlap_pos(seq_args *sa, int *list, int *count, const char *tag, int seq_len, int first_index, pr_append_str * nonfatal_err, pr_append_str *warning); static int _check_and_adjust_1_interval(const char *, int num, interval_array_t, int, int first_index, pr_append_str *err, seq_args *, pr_append_str *warning, int); static void sort_primer_array(oligo_array *); static void add_pair(const primer_pair *, pair_array_t *); static double align(const char *, const char*, const dpal_args *a); static double align_thermod(const char *, const char *, const thal_args *a); static int characterize_pair(p3retval *p, const p3_global_settings *, const seq_args *, int, int, int, primer_pair *, const dpal_arg_holder*, const thal_arg_holder*, int update_stats); static void choose_pair_or_triple(p3retval *, const p3_global_settings *, const seq_args *, const dpal_arg_holder *, const thal_arg_holder *, const thal_arg_holder *, pair_array_t *); static int sequence_quality_is_ok(const p3_global_settings *, primer_rec *, oligo_type, const seq_args *, int, int, oligo_stats *global_oligo_stats, const args_for_one_oligo_or_primer *); static int choose_internal_oligo(p3retval *, const primer_rec *, const primer_rec *, int *, const seq_args *, const p3_global_settings *, const dpal_arg_holder *, const thal_arg_holder *); void compute_position_penalty(const p3_global_settings *, const seq_args *, primer_rec *, oligo_type); static p3retval *create_p3retval(void); static char dna_to_upper(char *, int); static int find_stop_codon(const char *, int, int); static void gc_and_n_content(int, int, const char *, primer_rec *); static int make_detection_primer_lists(p3retval *, const p3_global_settings *, const seq_args *, const dpal_arg_holder *, const thal_arg_holder *); static int make_complete_primer_lists(p3retval *retval, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, const thal_arg_holder *thal_oligo_arg_to_use); static int add_primers_to_check(p3retval *retval, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, const thal_arg_holder *thal_oligo_arg_to_use); static int pick_sequencing_primer_list(p3retval *retval, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use); static int make_internal_oligo_list(p3retval *, const p3_global_settings *, const seq_args *, const dpal_arg_holder *, const thal_arg_holder *); static int pick_only_best_primer(const int, const int, oligo_array *, const p3_global_settings *, const seq_args *, const dpal_arg_holder *, const thal_arg_holder *, p3retval *); static int pick_primer_range(const int, const int, int *, oligo_array *, const p3_global_settings *, const seq_args *, const dpal_arg_holder *, const thal_arg_holder *, p3retval *retval); static int add_one_primer(const char *, int *, oligo_array *, const p3_global_settings *, const seq_args *, const dpal_arg_holder *, const thal_arg_holder *, p3retval *); static int add_one_primer_by_position(int, int, int *, oligo_array *, const p3_global_settings *, const seq_args *, const dpal_arg_holder *, const thal_arg_holder *, p3retval *); static int pick_primers_by_position(const int, const int, int *, oligo_array *, const p3_global_settings *, const seq_args *, const dpal_arg_holder *, const thal_arg_holder *, p3retval *); static double obj_fn(const p3_global_settings *, primer_pair *); static int left_oligo_in_pair_overlaps_used_oligo(const primer_rec *left, const primer_pair *best_pair, int min_dist); static int right_oligo_in_pair_overlaps_used_oligo(const primer_rec *right, const primer_pair *best_pair, int min_dist); static int oligo_overlaps_interval(int, int, const interval_array_t, int); static void calc_and_check_oligo_features(const p3_global_settings *pa, primer_rec *, oligo_type, const dpal_arg_holder*, const thal_arg_holder*, const seq_args *, oligo_stats *, p3retval *, const char *); static void pr_append(pr_append_str *, const char *); static void pr_append_new_chunk(pr_append_str *x, const char *s); static int pair_spans_target(const primer_pair *, const seq_args *); static void pr_append_w_sep(pr_append_str *, const char *, const char *); static void* pr_safe_malloc(size_t x); static void* pr_safe_realloc(void *p, size_t x); static int compare_primer_pair(const void *, const void*); static int primer_rec_comp(const void *, const void *); static int print_list_header(FILE *, oligo_type, int, int, int); static int print_oligo(FILE *, const seq_args *, int, const primer_rec *, oligo_type, int, int,int); static char *strstr_nocase(char *, char *); static double p_obj_fn(const p3_global_settings *, primer_rec *, int ); static void oligo_compl(primer_rec *, const args_for_one_oligo_or_primer *po_args, oligo_stats *, const dpal_arg_holder *, const char *oligo_seq, const char *revc_oligo_seq ); static void oligo_compl_thermod(primer_rec *, /* TO DO 2012-06-01 -- update by removing last argument. */ const args_for_one_oligo_or_primer *po_args, oligo_stats *, const thal_arg_holder *, const char *oligo_seq, const char *revc_oligo_seq ); static void oligo_hairpin(primer_rec *, const args_for_one_oligo_or_primer *po_args, oligo_stats *, const thal_arg_holder *, const char *oligo_seq ); static void oligo_compute_sequence_and_reverse(primer_rec *, const seq_args *, oligo_type, int*, int*, char*, char*); static void oligo_repeat_library_mispriming(primer_rec *, const p3_global_settings *, const seq_args *, oligo_type, oligo_stats *, const dpal_arg_holder *); static void oligo_template_mispriming(primer_rec *, const p3_global_settings *, const seq_args *, oligo_type, oligo_stats *, const dpal_args *, const thal_args *); static int pair_repeat_sim(primer_pair *, const p3_global_settings *); static void free_repeat_sim_score(p3retval *); static void free_primer_repeat_sim_score(primer_rec *); /* edited by T. Koressaar for lowercase masking: */ static int is_lowercase_masked(int position, const char *sequence, primer_rec *h, oligo_stats *); static int primer_must_match(const p3_global_settings *pa, primer_rec *h, oligo_stats *stats, const char *input_oligo_seq, char *match_three_prime, char *match_five_prime); static int compare_nucleotides(const char a, const char b); static int test_must_match_parameters(char *test); static void set_retval_both_stop_codons(const seq_args *sa, p3retval *retval); /* Functions to set bitfield parameters for oligos (or primers) */ static void bf_set_overlaps_target(primer_rec *, int); static int bf_get_overlaps_target(const primer_rec *); static void bf_set_overlaps_excl_region(primer_rec *, int); static int bf_get_overlaps_excl_region(const primer_rec *); static void bf_set_infinite_pos_penalty(primer_rec *, int); static int bf_get_infinite_pos_penalty(const primer_rec *); /* Functions to record problems with oligos (or primers) */ static void initialize_op(primer_rec *); static void op_set_completely_written(primer_rec *); static void op_set_must_match_err(primer_rec *); static void op_set_too_many_ns(primer_rec *); static void op_set_overlaps_target(primer_rec *); static void op_set_high_gc_content(primer_rec *); static void op_set_low_gc_content(primer_rec *); static void op_set_high_tm(primer_rec *); static void op_set_low_tm(primer_rec *); static void op_set_overlaps_excluded_region(primer_rec *); static void op_set_not_in_any_ok_region(primer_rec *); static void op_set_high_self_any(primer_rec *oligo); static void op_set_high_self_end(primer_rec *oligo); static void op_set_high_hairpin_th(primer_rec *oligo); static void op_set_no_gc_glamp(primer_rec *); static void op_set_too_many_gc_at_end(primer_rec *); static void op_set_high_end_stability(primer_rec *); static void op_set_high_poly_x(primer_rec *); static void op_set_low_sequence_quality(primer_rec *); static void op_set_low_end_sequence_quality(primer_rec *oligo); static void op_set_high_similarity_to_non_template_seq(primer_rec *); static void op_set_high_similarity_to_multiple_template_sites(primer_rec *); static void op_set_overlaps_masked_sequence(primer_rec *); static void op_set_too_long(primer_rec *); static void op_set_too_short(primer_rec *); static void op_set_does_not_amplify_orf(primer_rec *); /* End functions to set problems in oligos */ /* Global static variables. */ static const char *primer3_copyright_char_star = "\n" "Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008\n" " 2009,2010,2011,2012\n" "Whitehead Institute for Biomedical Research, Steve Rozen\n" "(http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky\n" "All rights reserved.\n" "\n" " This file is part of the primer3 suite and libraries.\n" "\n" " The primer3 suite and libraries are free software;\n" " you can redistribute them and/or modify them under the terms\n" " of the GNU General Public License as published by the Free\n" " Software Foundation; either version 2 of the License, or (at\n" " your option) any later version.\n" "\n" " This software is distributed in the hope that it will be useful,\n" " but WITHOUT ANY WARRANTY; without even the implied warranty of\n" " MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the\n" " GNU General Public License for more details.\n" "\n" " You should have received a copy of the GNU General Public License\n" " along with this software (file gpl-2.0.txt in the source\n" " distribution); if not, write to the Free Software\n" " Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA\n" "\n" "THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS\n" "\"AS IS\" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT\n" "LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR\n" "A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT\n" "OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL,\n" "SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT\n" "LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE,\n" "DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY\n" "THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT\n" "(INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE\n" "OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.\n" "\n"; static const char *pr_program_name = "probably primer3_core"; static const int use_end_for_th_template_mispriming = 1; #define DEFAULT_OPT_GC_PERCENT PR_UNDEFINED_INT_OPT /* Set the program name */ void p3_set_program_name(const char *pname) { pr_program_name = pname; } /* ============================================================ */ /* BEGIN functions for global settings */ /* ============================================================ */ /* Allocate space for global settings and fill in defaults */ p3_global_settings * p3_create_global_settings() { p3_global_settings *r; if (!(r = (p3_global_settings *) malloc(sizeof(*r)))) { return NULL; } pr_set_default_global_args_2(r); return r; } /* Allocate space for global settings and fill in defaults */ p3_global_settings * p3_create_global_settings_default_version_1() { p3_global_settings *r; if (!(r = (p3_global_settings *) malloc(sizeof(*r)))) { return NULL; } pr_set_default_global_args_1(r); return r; } /* Free the space of global settings */ void p3_destroy_global_settings(p3_global_settings *a) { if (NULL != a) { if (NULL != a->p_args.must_match_five_prime) { free(a->p_args.must_match_five_prime); } if (NULL != a->p_args.must_match_three_prime) { free(a->p_args.must_match_three_prime); } if (NULL != a->o_args.must_match_five_prime) { free(a->o_args.must_match_five_prime); } if (NULL != a->o_args.must_match_three_prime) { free(a->o_args.must_match_three_prime); } destroy_seq_lib(a->p_args.repeat_lib); destroy_seq_lib(a->o_args.repeat_lib); free(a); } } static void pr_set_default_global_args_2(p3_global_settings *a) /* Write the default values for default_values=2 into the p3_global_settings struct */ { pr_set_default_global_args_1(a); a->tm_santalucia = santalucia_auto; a->salt_corrections = santalucia; a->thermodynamic_oligo_alignment = 1; a->thermodynamic_template_alignment = 0; a->p_args.divalent_conc = 1.5; a->p_args.dntp_conc = 0.6; a->lib_ambiguity_codes_consensus = 0; } /* Write the default values for default_values=1 into the p3_global_settings struct */ static void pr_set_default_global_args_1(p3_global_settings *a) { memset(a, 0, sizeof(*a)); /* Arguments for primers ================================= */ a->p_args.opt_size = 20; a->p_args.min_size = 18; a->p_args.max_size = 27; a->p_args.opt_tm = 60; a->p_args.min_tm = 57; a->p_args.max_tm = 63; a->p_args.min_gc = 20.0; a->p_args.opt_gc_content = DEFAULT_OPT_GC_PERCENT; a->p_args.max_gc = 80.0; a->p_args.salt_conc = 50.0; a->p_args.divalent_conc = 0.0; a->p_args.dntp_conc = 0.0; a->p_args.dna_conc = 50.0; a->p_args.num_ns_accepted = 0; a->p_args.max_self_any = 8.0; a->p_args.max_self_end = 3.0; a->p_args.max_self_any_th = 47.0; a->p_args.max_self_end_th = 47.0; a->p_args.max_hairpin_th = 47.0; a->p_args.max_poly_x = 5; a->p_args.max_repeat_compl = 12.0; a->p_args.min_quality = 0; a->p_args.min_end_quality = 0; a->p_args.max_template_mispriming = PR_UNDEFINED_ALIGN_OPT; a->p_args.max_template_mispriming_th = PR_UNDEFINED_ALIGN_OPT; /* The following apply only to primers (and not to internal oligos). */ a->gc_clamp = 0; a->max_end_gc = 5; /* Weights for objective functions for oligos and pairs. */ a->p_args.weights.compl_any = 0; a->p_args.weights.compl_any_th = 0; a->p_args.weights.compl_end = 0; a->p_args.weights.compl_end_th = 0; a->p_args.weights.end_quality = 0; a->p_args.weights.end_stability = 0; a->p_args.weights.gc_content_gt = 0; a->p_args.weights.gc_content_lt = 0; a->p_args.weights.hairpin_th = 0; a->p_args.weights.length_gt = 1; a->p_args.weights.length_lt = 1; a->p_args.weights.num_ns = 0; a->p_args.weights.pos_penalty = 1; a->p_args.weights.repeat_sim = 0; a->p_args.weights.seq_quality = 0; a->p_args.weights.temp_cutoff = 5; a->p_args.weights.temp_gt = 1; a->p_args.weights.temp_lt = 1; a->p_args.weights.template_mispriming = 0.0; a->p_args.weights.template_mispriming_th = 0.0; a->p_args.must_match_five_prime = NULL; a->p_args.must_match_three_prime = NULL; /* End of weights for objective functions for oligos and pairs. */ /* End of arguments for primers =========================== */ a->max_diff_tm = 100.0; a->tm_santalucia = breslauer_auto; a->salt_corrections = schildkraut; a->pair_compl_any = 8.0; a->pair_compl_end = 3.0; a->pair_compl_any_th = 47.0; a->pair_compl_end_th = 47.0; a->thermodynamic_oligo_alignment = 0; a->thermodynamic_template_alignment = 0; a->liberal_base = 0; a->primer_task = generic; a->pick_left_primer = 1; a->pick_right_primer = 1; a->pick_internal_oligo = 0; a->first_base_index = 0; a->num_return = 5; a->pr_min[0] = 100; a->pr_max[0] = 300; a->num_intervals = 1; a->pair_repeat_compl = 24.0; a->quality_range_min = 0; a->quality_range_max = 100; a->outside_penalty = PR_DEFAULT_OUTSIDE_PENALTY; a->inside_penalty = PR_DEFAULT_INSIDE_PENALTY; a->max_end_stability = 100.0; a->lowercase_masking = 0; a->product_max_tm = PR_DEFAULT_PRODUCT_MAX_TM; a->product_min_tm = PR_DEFAULT_PRODUCT_MIN_TM; a->product_opt_tm = PR_UNDEFINED_DBL_OPT; a->product_opt_size = PR_UNDEFINED_INT_OPT; a->pair_max_template_mispriming = PR_UNDEFINED_ALIGN_OPT; a->pair_max_template_mispriming_th = PR_UNDEFINED_ALIGN_OPT; a->o_args.opt_size = 20; a->o_args.min_size = 18; a->o_args.max_size = 27; a->o_args.opt_tm = 60.0; a->o_args.min_tm = 57.0; a->o_args.max_tm = 63.0; a->o_args.min_gc = 20.0; a->o_args.max_gc = 80.0; a->o_args.opt_gc_content = DEFAULT_OPT_GC_PERCENT; a->o_args.max_poly_x = 5; a->o_args.salt_conc = 50.0; a->o_args.divalent_conc = 0.0; a->o_args.dntp_conc = 0.0; a->o_args.dna_conc = 50.0; a->o_args.num_ns_accepted = 0; a->o_args.max_self_any = 12.0; a->o_args.max_self_end = 12.0; a->o_args.max_self_any_th = 47.0; a->o_args.max_self_end_th = 47.0; a->o_args.max_hairpin_th = 47.0; a->o_args.max_repeat_compl= 12.0; a->o_args.min_quality = 0; a->o_args.min_end_quality = 0; a->o_args.max_template_mispriming = PR_UNDEFINED_ALIGN_OPT; a->o_args.max_template_mispriming_th = PR_UNDEFINED_ALIGN_OPT; a->o_args.weights.temp_gt = 1; a->o_args.weights.temp_lt = 1; a->o_args.weights.length_gt = 1; a->o_args.weights.length_lt = 1; a->o_args.weights.gc_content_gt = 0; a->o_args.weights.gc_content_lt = 0; a->o_args.weights.compl_any = 0; a->o_args.weights.compl_end = 0; a->o_args.weights.compl_any_th = 0; a->o_args.weights.compl_end_th = 0; a->o_args.weights.hairpin_th = 0; a->o_args.weights.num_ns = 0; a->o_args.weights.repeat_sim = 0; a->o_args.weights.seq_quality = 0; a->o_args.weights.end_quality = 0; a->o_args.must_match_five_prime = NULL; a->o_args.must_match_three_prime = NULL; a->pr_pair_weights.primer_quality = 1; a->pr_pair_weights.io_quality = 0; a->pr_pair_weights.diff_tm = 0; a->pr_pair_weights.compl_any = 0; a->pr_pair_weights.compl_end = 0; a->pr_pair_weights.compl_any_th = 0; a->pr_pair_weights.compl_end_th = 0; a->pr_pair_weights.temp_cutoff = 5; a->pr_pair_weights.repeat_sim = 0; a->pr_pair_weights.product_tm_lt = 0; a->pr_pair_weights.product_tm_gt = 0; a->pr_pair_weights.product_size_lt = 0; a->pr_pair_weights.product_size_gt = 0; a->lib_ambiguity_codes_consensus = 1; /* Set to 1 for backward compatibility. This _NOT_ what one normally wants, since many libraries contain strings of N, which then match every oligo (very bad). */ a->min_left_three_prime_distance = -1; a->min_right_three_prime_distance = -1; a->sequencing.lead = 50; a->sequencing.spacing = 500; a->sequencing.interval = 250; a->sequencing.accuracy = 20; a->min_5_prime_overlap_of_junction = 7; a->min_3_prime_overlap_of_junction = 4; } /* Add a pair of integers to an array of intervals */ int p3_add_to_interval_array(interval_array_t2 *interval_arr, int i1, int i2) { int c = interval_arr->count; if (c >= PR_MAX_INTERVAL_ARRAY) return 1; interval_arr->pairs[c][0] = i1; interval_arr->pairs[c][1] = i2; interval_arr->count++; return 0; } /* Add 2 pairs of integers to an array of 2 intervals */ int p3_add_to_2_interval_array(interval_array_t4 *interval_arr, int i1, int i2, int i3, int i4) { int c = interval_arr->count; if (c >= PR_MAX_INTERVAL_ARRAY) return 1; /* for a region either both values are given, or none is given */ if (((i1 == -1) && (i2 != -1)) || ((i1 != -1) && (i2 == -1))) return 2; if (((i3 == -1) && (i4 != -1)) || ((i3 != -1) && (i4 == -1))) return 2; interval_arr->left_pairs[c][0] = i1; interval_arr->left_pairs[c][1] = i2; interval_arr->right_pairs[c][0] = i3; interval_arr->right_pairs[c][1] = i4; if ((i1 == -1) && (i2 == -1)) interval_arr->any_left = 1; if ((i3 == -1) && (i4 == -1)) interval_arr->any_right = 1; if ((i1 == -1) && (i2 == -1) && (i3 == -1) && (i4 == -1)) interval_arr->any_pair = 1; interval_arr->count++; return 0; } /* ============================================================ */ /* END functions for global settings */ /* ============================================================ */ int interval_array_t2_count(const interval_array_t2 *array) { return array->count; } const int * interval_array_t2_get_pair(const interval_array_t2 *array, int i) { if (i > array->count) abort(); if (i < 0) abort(); return array->pairs[i]; } /* ============================================================ */ /* BEGIN functions for p3retval */ /* ============================================================ */ /* Allocate a new primer3 state. Return NULL if out of memory. Assuming malloc sets errno to ENOMEM according to Unix98, set errno to ENOMEM on out-of-memory error. */ static p3retval * create_p3retval(void) { p3retval *state = (p3retval *)malloc(sizeof(*state)); if (!state) return NULL; state->fwd.oligo = (primer_rec *) malloc(sizeof(*state->fwd.oligo) * INITIAL_LIST_LEN); state->rev.oligo = (primer_rec *) malloc(sizeof(*state->rev.oligo) * INITIAL_LIST_LEN); state->intl.oligo = (primer_rec *) malloc(sizeof(*state->intl.oligo) * INITIAL_LIST_LEN); if (state->fwd.oligo == NULL || state->rev.oligo == NULL || state->intl.oligo == NULL) { free(state); return NULL; } state->fwd.storage_size = INITIAL_LIST_LEN; state->rev.storage_size = INITIAL_LIST_LEN; state->intl.storage_size = INITIAL_LIST_LEN; state->fwd.num_elem = 0; state->rev.num_elem = 0; state->intl.num_elem = 0; state->fwd.type = OT_LEFT; state->intl.type = OT_INTL; state->rev.type = OT_RIGHT; state->best_pairs.storage_size = 0; state->best_pairs.pairs = NULL; state->best_pairs.num_pairs = 0; init_pr_append_str(&state->glob_err); init_pr_append_str(&state->per_sequence_err); init_pr_append_str(&state->warnings); memset(&state->fwd.expl, 0, sizeof(state->fwd.expl)); memset(&state->rev.expl, 0, sizeof(state->rev.expl)); memset(&state->intl.expl, 0, sizeof(state->intl.expl)); memset(&state->best_pairs.expl, 0, sizeof(state->best_pairs.expl)); return state; } /* ============================================================ */ /* BEGIN functions for dpal_arg_holder */ /* ============================================================ */ /* Create the dpal arg holder */ dpal_arg_holder * create_dpal_arg_holder () { dpal_arg_holder *h = (dpal_arg_holder *) pr_safe_malloc(sizeof(dpal_arg_holder)); h->local = (dpal_args *) pr_safe_malloc(sizeof(*h->local)); set_dpal_args(h->local); h->local->flag = DPAL_LOCAL; h->end = (dpal_args *) pr_safe_malloc(sizeof(*h->end)); set_dpal_args(h->end); h->end->flag = DPAL_GLOBAL_END; h->local_end = (dpal_args *) pr_safe_malloc(sizeof(*h->local_end)); set_dpal_args(h->local_end); h->local_end->flag = DPAL_LOCAL_END; h->local_ambig = (dpal_args *) pr_safe_malloc(sizeof(*h->local_ambig)); *h->local_ambig = *h->local; PR_ASSERT(dpal_set_ambiguity_code_matrix(h->local_ambig)); h->local_end_ambig = (dpal_args *) pr_safe_malloc(sizeof(*h->local_end_ambig)); *h->local_end_ambig = *h->local_end; PR_ASSERT(dpal_set_ambiguity_code_matrix(h->local_end_ambig)); return h; } /* Free the dpal arg holder */ void destroy_dpal_arg_holder(dpal_arg_holder *h) { if (NULL != h) { free(h->local); free(h->end); free(h->local_end); free(h->local_ambig); free(h->local_end_ambig); free(h); } } /* This is a static variable that is initialized once in choose_primers(). We make this variable have 'file' scope, we do not have to remember to free the associated storage after each call to choose_primers(). */ static dpal_arg_holder *dpal_arg_to_use = NULL; /* ============================================================ */ /* END functions for dpal_arg_holder */ /* ============================================================ */ /* ============================================================ */ /* BEGIN functions for thal_arg_holder */ /* ============================================================ */ /* Create the thal arg holder */ thal_arg_holder * create_thal_arg_holder (const args_for_one_oligo_or_primer *po_args) { thal_arg_holder *h = (thal_arg_holder *) pr_safe_malloc(sizeof(thal_arg_holder)); h->any = (thal_args *) pr_safe_malloc(sizeof(*h->any)); set_thal_default_args(h->any); h->any->type = thal_any; h->any->mv = po_args->salt_conc; h->any->dv = po_args->divalent_conc; h->any->dntp = po_args->dntp_conc; h->any->dna_conc = po_args->dna_conc; h->end1 = (thal_args *) pr_safe_malloc(sizeof(*h->end1)); set_thal_default_args(h->end1); h->end1->type = thal_end1; h->end1->mv = po_args->salt_conc; h->end1->dv = po_args->divalent_conc; h->end1->dntp = po_args->dntp_conc; h->end1->dna_conc = po_args->dna_conc; h->end2 = (thal_args *) pr_safe_malloc(sizeof(*h->end2)); set_thal_default_args(h->end2); h->end2->type = thal_end2; h->end2->mv = po_args->salt_conc; h->end2->dv = po_args->divalent_conc; h->end2->dntp = po_args->dntp_conc; h->end2->dna_conc = po_args->dna_conc; h->hairpin_th = (thal_args *) pr_safe_malloc(sizeof(*h->hairpin_th)); set_thal_default_args(h->hairpin_th); h->hairpin_th->type = thal_hairpin; h->hairpin_th->mv = po_args->salt_conc; h->hairpin_th->dv = po_args->divalent_conc; h->hairpin_th->dntp = po_args->dntp_conc; h->hairpin_th->dna_conc = po_args->dna_conc; h->hairpin_th->dimer = 0; return h; } /* Free the thal arg holder */ void destroy_thal_arg_holder(thal_arg_holder *h) { if (NULL != h) { free(h->any); free(h->end1); free(h->end2); free(h->hairpin_th); free(h); } } static thal_arg_holder *thal_arg_to_use = NULL; static thal_arg_holder *thal_oligo_arg_to_use = NULL; /* ============================================================ */ /* END functions for thal_arg_holder */ /* ============================================================ */ /* Deallocate a primer3 state */ void destroy_p3retval(p3retval *state) { if (!state) return; free_repeat_sim_score(state); free(state->fwd.oligo); free(state->rev.oligo); free(state->intl.oligo); if (state->best_pairs.storage_size != 0 && state->best_pairs.pairs) free(state->best_pairs.pairs); destroy_pr_append_str_data(&state->glob_err); destroy_pr_append_str_data(&state->per_sequence_err); destroy_pr_append_str_data(&state->warnings); free(state); } void destroy_dpal_thal_arg_holder() { if(dpal_arg_to_use) destroy_dpal_arg_holder(dpal_arg_to_use); if(thal_arg_to_use) destroy_thal_arg_holder(thal_arg_to_use); if(thal_oligo_arg_to_use) destroy_thal_arg_holder(thal_oligo_arg_to_use); } const oligo_array * p3_get_rv_fwd(const p3retval *r) { return &r->fwd; } const oligo_array * p3_get_rv_intl(const p3retval *r) { return &r->intl; } const oligo_array * p3_get_rv_rev(const p3retval *r) { return &r->rev; } const pair_array_t * p3_get_rv_best_pairs(const p3retval *r) { return &r->best_pairs; } /* ============================================================ */ /* END functions for p3retval */ /* ============================================================ */ /* ============================================================ */ /* BEGIN functions for seq_arg_holder */ /* ============================================================ */ /* Create and initialize a seq_args data structure */ seq_args * create_seq_arg() { seq_args *r = (seq_args *) malloc(sizeof(*r)); if (NULL == r) return NULL; /* Out of memory */ memset(r, 0, sizeof(*r)); r->start_codon_pos = PR_DEFAULT_START_CODON_POS; r->incl_l = -1; /* Indicates logical NULL. */ r->force_left_start = PR_NULL_FORCE_POSITION; /* Indicates logical NULL. */ r->force_left_end = PR_NULL_FORCE_POSITION; /* Indicates logical NULL. */ r->force_right_start = PR_NULL_FORCE_POSITION; /* Indicates logical NULL. */ r->force_right_end = PR_NULL_FORCE_POSITION; /* Indicates logical NULL. */ r->primer_overlap_junctions_count = 0; r->n_quality = 0; r->quality = NULL; return r; } /* Free a seq_arg data structure */ void destroy_seq_args(seq_args *sa) { if (NULL == sa) return; free(sa->internal_input); free(sa->left_input); free(sa->right_input); free(sa->sequence); free(sa->quality); free(sa->trimmed_seq); /* edited by T. Koressaar for lowercase masking */ free(sa->trimmed_orig_seq); free(sa->upcased_seq); free(sa->upcased_seq_r); free(sa->sequence_name); free(sa); } /* ============================================================ */ /* END functions for seq_arg_holder */ /* ============================================================ */ /* Function used to de-allocate the memory used by the hash maps allocated in choose_pair_or_triple. */ static void free_pair_memory(int rev_num_elem) { std::hash_map *hmap; std::hash_map::iterator it; primer_pair *pp; int i; free(max_j_seen); for (i=0; ibegin(); it!=hmap->end(); it++) { pp = it->second; if (pp != NULL) delete pp; } delete hmap; } } free(pairs); } /* ============================================================ */ /* BEGIN choose_primers() */ /* The main primer3 interface */ /* See libprimer3.h for documentation. */ /* ============================================================ */ p3retval * choose_primers(const p3_global_settings *pa, seq_args *sa) { /* Create retval and set were to find the results */ p3retval *retval = create_p3retval(); if (retval == NULL) return NULL; PR_ASSERT(NULL != pa); PR_ASSERT(NULL != sa); if (pa->dump) { printf("Start of choose_primers:\n"); p3_print_args(pa, sa) ; } /* Set the general output type */ if (pa->pick_left_primer && pa->pick_right_primer) { retval->output_type = primer_pairs; } else { retval->output_type = primer_list; } if (pa->primer_task == pick_primer_list || pa->primer_task == pick_sequencing_primers) { retval->output_type = primer_list; } /* * For catching ENOMEM. WARNING: We can only use longjmp to escape * from errors that have been called through choose_primers(). * Therefore, if we subsequently update other static functions in * this file to have external linkage then we need to check whether * they call (or use functions that in turn call) longjmp to handle * ENOMEM. */ if (setjmp(_jmp_buf) != 0) { /* Check if this was a thermodynamic alignment length error. */ if (thermodynamic_alignment_length_error == 1) { thermodynamic_alignment_length_error = 0; /* Set per sequence error */ pr_append_new_chunk(&retval->per_sequence_err, thermodynamic_alignment_length_error_msg); free(thermodynamic_alignment_length_error_msg); thermodynamic_alignment_length_error_msg = NULL; /* Other necessary cleanup. */ free_pair_memory(retval->rev.num_elem); return retval; } /* This was a memory error. */ destroy_p3retval(retval); return NULL; /* If we get here, that means errno should be ENOMEM. */ } /* Change some parameters to fit the task */ _adjust_seq_args(pa, sa, &retval->per_sequence_err,&retval->warnings); if (pa->dump) { printf("After _adjust_seq_args\n"); p3_print_args(pa, sa) ; } if (!pr_is_empty(&retval->per_sequence_err)) return retval; /* TO DO -- move the check below to pr_data_control and issue a warning if it fails. */ /* if (pa->p_args.min_quality != 0 && pa->p_args.min_end_quality < pa->p_args.min_quality) ... issue warning ... pa->p_args.min_end_quality = pa->p_args.min_quality; */ /* Check if the input in sa and pa makes sense */ if (_pr_data_control(pa, sa, &retval->glob_err, /* Fatal errors */ &retval->per_sequence_err, /* Non-fatal errors */ &retval->warnings ) !=0 ) { return retval; } set_retval_both_stop_codons(sa, retval); /* Set the parameters for alignment functions if dpal_arg_to_use, a static variable that has 'file' scope, has not yet been initialized. */ if (dpal_arg_to_use == NULL) dpal_arg_to_use = create_dpal_arg_holder(); if(thal_arg_to_use == NULL) { thal_arg_to_use = create_thal_arg_holder(&pa->p_args); } else { destroy_thal_arg_holder(thal_arg_to_use); thal_arg_to_use = create_thal_arg_holder(&pa->p_args); } if(thal_oligo_arg_to_use == NULL) { thal_oligo_arg_to_use = create_thal_arg_holder(&pa->o_args); } else { destroy_thal_arg_holder(thal_oligo_arg_to_use); thal_oligo_arg_to_use = create_thal_arg_holder(&pa->o_args); } if (pa->primer_task == pick_primer_list) { make_complete_primer_lists(retval, pa, sa, dpal_arg_to_use,thal_arg_to_use,thal_oligo_arg_to_use); } else if (pa->primer_task == pick_sequencing_primers) { pick_sequencing_primer_list(retval, pa, sa, dpal_arg_to_use,thal_arg_to_use); } else if (pa->primer_task == check_primers) { add_primers_to_check(retval, pa, sa, dpal_arg_to_use, thal_arg_to_use, thal_oligo_arg_to_use); } else { /* The general way to pick primers */ /* Populate the forward and reverse primer lists */ if (make_detection_primer_lists(retval, pa, sa, dpal_arg_to_use,thal_arg_to_use) != 0) { /* There was an error */ return retval; } /* Populate the internal oligo lists */ if ( pa->pick_internal_oligo) { if (make_internal_oligo_list(retval, pa, sa, dpal_arg_to_use,thal_oligo_arg_to_use) != 0) { /* There was an error*/ return retval; } } } if (pa->pick_right_primer && (pa->primer_task != pick_sequencing_primers)) sort_primer_array(&retval->rev); if (pa->pick_left_primer && (pa->primer_task != pick_sequencing_primers)) sort_primer_array(&retval->fwd); /* If we are returning a list of internal oligos, sort them by their 'goodness'. We do not care if these are sorted if we end up in choose_pair_or_triple(), since this calls choose_internal_oligo(), which selects the best internal oligo for a given primer pair. */ if (retval->output_type == primer_list && pa->pick_internal_oligo == 1) sort_primer_array(&retval->intl); /* Select primer pairs if needed */ if (retval->output_type == primer_pairs) { choose_pair_or_triple(retval, pa, sa, dpal_arg_to_use, thal_arg_to_use, thal_oligo_arg_to_use, &retval->best_pairs); } if (pa->dump) { printf("End of choose_primers:\n"); p3_print_args(pa, sa) ; } return retval; } /* ============================================================ */ /* END choose_primers() */ /* ============================================================ */ /* ============================================================ */ /* BEGIN choose_pair_or_triple This function uses retval->fwd and retval->rev and updates the oligos in these array. This function posibly uses retval->intl and updates its elements via choose_internal_oligo(). This function examines primer pairs or triples to find pa->num_return pairs or triples to return. Results are returned in best_pairs and in retval->best_pairs.expl */ /* ============================================================ */ static void choose_pair_or_triple(p3retval *retval, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, const thal_arg_holder *thal_oligo_arg_to_use, pair_array_t *best_pairs) { int i,j; /* Loop index. */ int n_int; /* Index of the internal oligo */ /*int *max_j_seen; */ /* The maximum value of j (loop index for forward primers) that has been examined for every reverse primer index (i) -- global variable now */ int update_stats = 1; /* Flag to indicate whether pair_stats should be updated. */ primer_pair h; /* The current pair which is being evaluated. */ primer_pair the_best_pair; /* The best pair is being "remembered". */ pair_stats *pair_expl = &retval->best_pairs.expl; /* For statistics */ int product_size_range_index = 0; int trace_me = 0; int the_best_i, the_best_j; /* Hash maps used to store pairs that were computed */ /* std::hash_map **pairs; */ /* pairs is an array of pointers to hash maps. It will be indexed by the indices of the reverse primers in retval->rev. -- global var now */ std::hash_map *hmap, *best_hmap = NULL; /* hmap and best_hmap will be pointers to hash maps also pointed to by elements of pairs. */ std::hash_map::iterator it; primer_pair *pp, *best_pp = NULL; int pair_found = 0; pairs = (std::hash_map**) calloc (retval->rev.num_elem, sizeof(std::hash_map*)); if (!pairs) longjmp(_jmp_buf, 1); memset(&the_best_pair, 0, sizeof(the_best_pair)); max_j_seen = (int *) malloc(sizeof(int) * retval->rev.num_elem); for (i = 0; i < retval->rev.num_elem; i++) max_j_seen[i] = -1; /* Pick pairs till we have enough. */ while(1) { /* FIX ME, is this memset really needed? Apparently slow */ memset(&the_best_pair, 0, sizeof(the_best_pair)); the_best_i = -1; the_best_j = -1; /* To start put penalty to the maximum */ the_best_pair.pair_quality = DBL_MAX; /* Iterate over the reverse primers. */ for (i = 0; i < retval->rev.num_elem; i++) { hmap = pairs[i]; /* Pairs[i] is NULL if there has never been an assignment to pairs[i] because pairs was allocated by calloc, which sets the allocated memory to 0. */ /* Only use a primer that *might be* legal or that the caller has provided and specified as "must use". Primers are *NOT* FULLY ASSESSED until the call to characterize_pair(), in order to avoid expensive computations (mostly alignments) unless necessary. */ if (!OK_OR_MUST_USE(&retval->rev.oligo[i])) { /* Can free the memory used by the hmap associated to this reverse primer */ if (hmap) { for (it=hmap->begin(); it!=hmap->end(); it++) { /* it->second is the second element (i.e. the 'value', as opposed to the 'key'). */ pp = it->second; delete pp; } if (hmap == best_hmap) best_hmap = NULL; delete hmap; hmap = NULL; pairs[i] = NULL; } continue; } /* If the pair cannot be better than the one already * selected, then we can skip remaining reverse primers */ if (pa->pr_pair_weights.primer_quality * (retval->rev.oligo[i].quality + retval->fwd.oligo[0].quality) > the_best_pair.pair_quality) { break; } if (retval->rev.oligo[i].overlaps) { /* The stats will not keep track of the pair correctly after the first pass, because an oligo might have been legal on one pass but become illegal on a subsequent pass. */ if (update_stats) { if (trace_me) fprintf(stderr, "i=%d, j=%d, overlaps_oligo_in_better_pair++\n", i, j); pair_expl->overlaps_oligo_in_better_pair++; } /* Can free the memory used by the hmap associated to this reverse primer */ if (hmap) { for (it=hmap->begin(); it!=hmap->end(); it++) { /* it->second is the second element (i.e. the 'value', as opposed to the 'key'). */ pp = it->second; delete pp; } if (hmap == best_hmap) best_hmap = NULL; delete hmap; hmap = NULL; pairs[i] = NULL; } continue; } /* Loop over forward primers */ for (j=0; jfwd.num_elem; j++) { /* We check the reverse oligo again, because we may have determined that it is "not ok", even though (as a far as we knew), it was ok above. */ if (!OK_OR_MUST_USE(&retval->rev.oligo[i])) { /* Can free the memory used by the hmap associated to this reverse primer */ if (hmap) { for (it=hmap->begin(); it!=hmap->end(); it++) { /* it->second is the second element (i.e. the 'value', as opposed to the 'key'). */ pp = it->second; delete pp; } if (hmap == best_hmap) best_hmap = NULL; delete hmap; hmap = NULL; pairs[i] = NULL; } break; } /* Only use a primer that is legal, or that the caller has provided and specified as "must use". */ if (!OK_OR_MUST_USE(&retval->fwd.oligo[j])) continue; /* If the pair cannot be better than the one already * selected, then we can skip remaining forward primers * for this reverse primer */ if (pa->pr_pair_weights.primer_quality * (retval->fwd.oligo[j].quality + retval->rev.oligo[i].quality) > the_best_pair.pair_quality) { break; } /* Need to have this here because if we break just above, then, at a later iteration, we may need to examine the oligo pair with reverse oligo at i and forward oligo at j. */ update_stats = 0; if (j > max_j_seen[i]) { if (trace_me) fprintf(stderr, "updates ON: i=%d, j=%d, max_j_seen[%d]=%d\n", i, j, i, max_j_seen[i]); max_j_seen[i] = j; if (trace_me) fprintf(stderr, "max_j_seen[%d] --> %d\n", i, max_j_seen[i]); if (trace_me) fprintf(stderr, "updates on\n"); update_stats = 1; } if (retval->fwd.oligo[j].overlaps) { /* The stats will not keep track of the pair correctly after the first pass, because an oligo might have been legal on one pass but become illegal on a subsequent pass. */ if (update_stats) { if (trace_me) fprintf(stderr, "i=%d, j=%d, overlaps_oligo_in_better_pair++\n", i, j); pair_expl->overlaps_oligo_in_better_pair++; } continue; } /* Some simple checks first, before searching the hashmap */ int must_use = 0; if ((pa->primer_task == check_primers) || ((retval->fwd.oligo[j].must_use != 0) && (retval->rev.oligo[i].must_use != 0))) { must_use = 1; } /* Determine if overlap with an overlap point is required, and if so, whether one of the primers in the pairs overlaps that point. */ if ((sa->primer_overlap_junctions_count > 0) && !(retval->rev.oligo[i].overlaps_overlap_position || retval->fwd.oligo[j].overlaps_overlap_position) ) { if (update_stats) { pair_expl->considered++; pair_expl->does_not_overlap_a_required_point++; } if (!must_use) continue; } /* Check product size now */ double product_size = retval->rev.oligo[i].start - retval->fwd.oligo[j].start+1; if (product_size < pa->pr_min[product_size_range_index] || product_size > pa->pr_max[product_size_range_index]) { if (update_stats) { /* This line NEW */ if (!must_use) pair_expl->considered++; pair_expl->product++; } if (!must_use) continue; } /* Check if pair was already computed */ pair_found = 0; if (hmap) { it = hmap->find(j); if (it != hmap->end()) { pair_found = 1; /* it->second is the second element (i.e. the 'value', as opposed to the 'key'). */ pp = it->second; if (pp) { /* The pair was computed, it isn't illegal and it wasn't selected yet */ if (update_stats) { pair_expl->considered++; if (trace_me) fprintf(stderr, "ok++\n"); pair_expl->ok++; } /* Check if this is a better pair */ if (compare_primer_pair(pp, &the_best_pair) < 0) { the_best_pair = *pp; the_best_i = i; the_best_j = j; best_hmap = hmap; best_pp = pp; } /* There cannot be a better pair */ if (the_best_pair.pair_quality == 0) { break; } } /* else - pp is NULL - it's illegal or already selected */ } } else { /* Create this hashmap */ hmap = new std::hash_map; if (!hmap) longjmp(_jmp_buf, 1); pairs[i] = hmap; } if (!pair_found) { /* Characterize the pair. h is initialized by this call. */ int tmp = characterize_pair(retval, pa, sa, j, i, product_size_range_index, &h, dpal_arg_to_use, thal_arg_to_use, update_stats); if (tmp == PAIR_OK) { /* Choose internal oligo if needed */ if (pa->pick_right_primer && pa->pick_left_primer && pa->pick_internal_oligo) { if (choose_internal_oligo(retval, h.left, h.right, &n_int, sa, pa, dpal_arg_to_use, thal_oligo_arg_to_use)!=0) { /* We were UNable to choose an internal oligo. */ if (update_stats) { pair_expl->internal++; } /* Mark the pair as not good - the entry in the hash map will be a NULL */ (*hmap)[j] = NULL; continue; } else { /* We DID choose an internal oligo, and we set h.intl to point to it. */ h.intl = &retval->intl.oligo[n_int]; } } if (update_stats) { if (trace_me) fprintf(stderr, "ok++\n"); pair_expl->ok++; } /* Calculate the pair penalty */ h.pair_quality = obj_fn(pa, &h); PR_ASSERT(h.pair_quality >= 0.0); /* Save the pair */ pp = new primer_pair; if (!pp) longjmp(_jmp_buf, 1); *pp = h; (*hmap)[j] = pp; /* The current pair (h) is the new best pair if it is better than the best pair so far. */ if (compare_primer_pair(&h, &the_best_pair) < 0) { the_best_pair = h; the_best_i = i; the_best_j = j; best_hmap = hmap; best_pp = pp; } /* There cannot be a better pair */ if (the_best_pair.pair_quality == 0) { break; } } else if (tmp == PAIR_FAILED) { /* Illegal pair */ (*hmap)[j] = NULL; } } } /* for (j=0; jfwd.num_elem; j++) -- inner loop */ /* Check if there cannot be a better pair than best found */ if (the_best_pair.pair_quality == 0) { break; } } /* for (i = 0; i < retval->rev.num_elem; i++) --- outer loop */ if (the_best_pair.pair_quality == DBL_MAX){ /* No pair was found. Try another product-size-range, if one exists. */ product_size_range_index++; /* Re-set the high-water marks for the indices for reverse and forward primers: */ for (i = 0; i < retval->rev.num_elem; i++) max_j_seen[i] = -1; if (!(product_size_range_index < pa->num_intervals)) { /* We ran out of product-size-ranges. Exit the while loop. */ break; /* Our bookkeeping was incorrect unless the assertion below is true. If num_intervals > 1 or min_three_*_prime_distance > -1 the assertion below might not be true. */ PR_ASSERT(!((pa->num_intervals == 1) && ((pa->min_left_three_prime_distance == -1) || (pa->min_right_three_prime_distance == -1))) || (best_pairs->num_pairs == pair_expl->ok)); } } else { /* Store the best primer for output */ if (trace_me) fprintf(stderr, "ADD pair i=%d, j=%d\n", the_best_i, the_best_j); add_pair(&the_best_pair, best_pairs); /* Mark the pair as already selected */ delete best_pp; (*best_hmap)[the_best_j] = NULL; /* Update the overlaps flags */ for (i = 0; i < retval->rev.num_elem; i++) { if (right_oligo_in_pair_overlaps_used_oligo(&retval->rev.oligo[i], &the_best_pair, pa->min_right_three_prime_distance)) { retval->rev.oligo[i].overlaps = 1; } } for (j = 0; j < retval->fwd.num_elem; j++) { if (left_oligo_in_pair_overlaps_used_oligo(&retval->fwd.oligo[j], &the_best_pair, pa->min_left_three_prime_distance)) { retval->fwd.oligo[j].overlaps = 1; } } /* If we have enough then stop the while loop */ if (pa->num_return == best_pairs->num_pairs) { break; } } } /* end of while(continue_trying == 1) */ /* Final cleanup of dynamically allocated storage for this function. */ free_pair_memory(retval->rev.num_elem); } /* ============================================================ */ /* END choose_pair_or_triple */ /* ============================================================ */ /* ============================================================ */ /* BEGIN choose_internal_oligo */ /* Choose best internal oligo for given pair of left and right primers. */ /* ============================================================ */ static int choose_internal_oligo(p3retval *retval, const primer_rec *left, const primer_rec *right, int *nm, const seq_args *sa, const p3_global_settings *pa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use ) { int i,k; double min; char oligo_seq[MAX_PRIMER_LENGTH+1], revc_oligo_seq[MAX_PRIMER_LENGTH+1]; primer_rec *h; min = 1000000.; i = -1; for (k=0; k < retval->intl.num_elem; k++) { h = &retval->intl.oligo[k]; /* h is the record for the oligo currently under consideration */ if ((h->start > (left->start + (left->length-1))) && ((h->start + (h->length-1)) < (right->start-right->length+1)) && (h->quality < min) && (OK_OR_MUST_USE(h))) { if (h->self_any == ALIGN_SCORE_UNDEF && pa->thermodynamic_oligo_alignment==0) { _pr_substr(sa->trimmed_seq, h->start, h->length, oligo_seq); p3_reverse_complement(oligo_seq, revc_oligo_seq); oligo_compl(h, &pa->o_args, &retval->intl.expl, dpal_arg_to_use, oligo_seq, revc_oligo_seq); if (!OK_OR_MUST_USE(h)) continue; } if (h->self_any == ALIGN_SCORE_UNDEF && pa->thermodynamic_oligo_alignment==1) { _pr_substr(sa->trimmed_seq, h->start, h->length, oligo_seq); p3_reverse_complement(oligo_seq, revc_oligo_seq); oligo_compl_thermod(h, &pa->o_args, &retval->intl.expl, thal_oligo_arg_to_use, oligo_seq, oligo_seq); if (!OK_OR_MUST_USE(h)) continue; } if(h->hairpin_th == ALIGN_SCORE_UNDEF && pa->thermodynamic_oligo_alignment==1) { _pr_substr(sa->trimmed_seq, h->start, h->length, oligo_seq); oligo_hairpin(h, &pa->o_args, &retval->intl.expl, thal_oligo_arg_to_use, oligo_seq); if (!OK_OR_MUST_USE(h)) continue; } if (h->repeat_sim.score == NULL) { oligo_repeat_library_mispriming(h, pa, sa, OT_INTL, &retval->intl.expl, dpal_arg_to_use); if (!OK_OR_MUST_USE(h)) continue; } min = h->quality; i=k; } /* if ((h->start.... */ } /* for (k=0;..... */ *nm = i; if(*nm < 0) return 1; return 0; } /* ============================================================ */ /* END choose_internal_oligo */ /* ============================================================ */ /* Translate the values in the stats struct into an warning string. Call this function only if the 'stat's contains the _errors_ associated with a given primer i.e. that primer was supplied by the caller and pick_anyway is set. */ void add_must_use_warnings(pr_append_str *warning, const char* text, const oligo_stats *stats) { const char *sep = "/"; pr_append_str s; s.data = NULL; s.storage_size = 0; if (stats->size_min) pr_append_w_sep(&s, sep, "Too short"); if (stats->size_max) pr_append_w_sep(&s, sep, "Too long"); if (stats->ns) pr_append_w_sep(&s, sep, "Too many Ns"); if (stats->target) pr_append_w_sep(&s, sep, "Overlaps Target"); if (stats->excluded) pr_append_w_sep(&s, sep, "Overlaps Excluded Region"); if (stats->gc) pr_append_w_sep(&s, sep, "Unacceptable GC content"); if (stats->gc_clamp) pr_append_w_sep(&s, sep, "No GC clamp"); if (stats->temp_min) pr_append_w_sep(&s, sep, "Tm too low"); if (stats->temp_max) pr_append_w_sep(&s, sep, "Tm too high"); if (stats->compl_any) pr_append_w_sep(&s, sep, "High self complementarity"); if (stats->compl_end) pr_append_w_sep(&s, sep, "High end self complementarity"); if (stats->hairpin_th) pr_append_w_sep(&s, sep, "High hairpin stability (thermod. approach)"); if (stats->repeat_score) pr_append_w_sep(&s, sep, "High similarity to mispriming or mishyb library"); if (stats->poly_x) pr_append_w_sep(&s, sep, "Long poly-X"); if (stats->seq_quality) pr_append_w_sep(&s, sep, "Low sequence quality"); if (stats->stability) pr_append_w_sep(&s, sep, "High 3' stability"); if (stats->no_orf) pr_append_w_sep(&s, sep, "Would not amplify any ORF"); if (stats->not_in_any_left_ok_region) pr_append_w_sep(&s, sep, "Not in any ok left region"); if (stats->not_in_any_right_ok_region) pr_append_w_sep(&s, sep, "Not in any ok right region"); /* edited by T. Koressaar for lowercase masking: */ if (stats->gmasked) pr_append_w_sep(&s, sep, "Masked with lowercase letter"); if (stats->must_match_fail) pr_append_w_sep(&s, sep, "Failed must_match requirements"); if (s.data) { pr_append_new_chunk(warning, text); pr_append(warning, " is unacceptable: "); pr_append(warning, s.data); free(s.data); } } /* Also see the documentation in librimer3.h, p3_global_settings.min_{left/right_}three_prime_distance. If min_dist == -1, return 0 If min_dist == 0: Return 1 if the left or the right primer is identical to the left or right primer (respectively) in best_pair. Otherwise return 0. If min_dist > 0: Return 1 if EITHER: (1) The 3' end of the left primer in pair is < min_dist from the 3' end of the left primer in best_pair OR (2) The 3' end of the right primer in pair is < min_dist from the 3' end of the right primer in best_pair */ static int right_oligo_in_pair_overlaps_used_oligo(const primer_rec *right, const primer_pair *best_pair, int min_dist) { int best_pos, pair_pos; if (min_dist == -1) return 0; best_pos = best_pair->right->start - best_pair->right->length + 1; pair_pos = right->start - right->length + 1; if ((abs(best_pos - pair_pos) < min_dist) && (min_dist != 0)) { return 1; } if ((best_pair->right->length == right->length) && (best_pair->right->start == right->start) && (min_dist == 0)) { return 1; } return 0; } static int left_oligo_in_pair_overlaps_used_oligo(const primer_rec *left, const primer_pair *best_pair, int min_dist) { int best_pos, pair_pos; if (min_dist == -1) return 0; best_pos = best_pair->left->start + best_pair->left->length - 1; pair_pos = left->start + left->length - 1; if ((abs(best_pos - pair_pos) < min_dist) && (min_dist != 0)) { return 1; } if ((best_pair->left->length == left->length) && (best_pair->left->start == left->start) && (min_dist == 0)) { return 1; } return 0; } /* Add 'pair' to 'retpair'; always appends. */ static void add_pair(const primer_pair *pair, pair_array_t *retpair) { /* If array is not initialised, initialise it with the size of pairs to return */ if (0 == retpair->storage_size) { retpair->storage_size = INITIAL_NUM_RETURN; retpair->pairs = (primer_pair *) pr_safe_malloc(retpair->storage_size * sizeof(*retpair->pairs)); } else { if (retpair->storage_size == retpair->num_pairs) { /* We need more space, so realloc double the space*/ retpair->storage_size *= 2; retpair->pairs = (primer_pair *) pr_safe_realloc(retpair->pairs, retpair->storage_size * sizeof(*retpair->pairs)); } } /* Copy the pair into the storage place */ retpair->pairs[retpair->num_pairs] = *pair; retpair->num_pairs++; } /* * Make lists of acceptable left and right primers. After return, the * lists are stored in retval->fwd.oligo and retval->rev.oligo and the * coresponding list sizes are stored in retval->fwd.num_elem and * retval->rev.num_elem. Return 1 if one of lists is empty or if * leftmost left primer and rightmost right primer do not provide * sufficient product size. */ static int make_detection_primer_lists(p3retval *retval, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use) { int left, right; int length, start; int i,n,/*k,*/ pr_min; int tar_l, tar_r, f_b, r_b; pair_stats *pair_expl = &retval->best_pairs.expl; /* To store the statistics for pairs */ /* Var to save the very left and very right primer */ left = right = 0; /* Set pr_min to the very smallest allowable product size. */ pr_min = INT_MAX; for (i=0; i < pa->num_intervals; i++) if(pa->pr_min[i] < pr_min) pr_min = pa->pr_min[i]; /* Get the length of the sequence */ PR_ASSERT(INT_MAX > (n=strlen(sa->trimmed_seq))); tar_r = 0; /* Target start position */ tar_l = n; /* Target length */ /* Iterate over target array */ for (i=0; i < sa->tar2.count; i++) { /* Select the rightmost target start */ if (sa->tar2.pairs[i][0] > tar_r) tar_r = sa->tar2.pairs[i][0]; /* Select the rightmost target end */ if (sa->tar2.pairs[i][0] + sa->tar2.pairs[i][1] - 1 < tar_l) tar_l = sa->tar2.pairs[i][0] + sa->tar2.pairs[i][1] - 1; } if (_PR_DEFAULT_POSITION_PENALTIES(pa)) { if (0 == tar_r) tar_r = n; if (tar_l == n) tar_l = 0; } else { tar_r = n; tar_l = 0; } /* We use some global information to restrict the region of the input sequence in which we generate candidate oligos. */ if (retval->output_type == primer_list && pa->pick_left_primer == 1) f_b = n - 1; else if (tar_r - 1 < n - pr_min + pa->p_args.max_size - 1 && !(pa->pick_anyway && sa->left_input)) f_b=tar_r - 1; else f_b = n - pr_min + pa->p_args.max_size-1; if (pa->pick_left_primer) { /* We will need a left primer. */ left=n; right=0; length = f_b - pa->p_args.min_size + 1; start = pa->p_args.min_size - 1; /* Use the primer provided */ if (sa->left_input) { add_one_primer(sa->left_input, &left, &retval->fwd, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } /* Pick primers at one position */ else if(sa->force_left_start > -1 || sa->force_left_end > -1) { pick_primers_by_position(sa->force_left_start, sa->force_left_end, &left, &retval->fwd, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } /* Or pick all good in the given range */ else { pick_primer_range(start, length, &left, &retval->fwd, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } } /* if (pa->pick_left_primer) */ if (retval->output_type == primer_list && pa->pick_right_primer == 1) r_b = 0; else if (tar_l+1>pr_min - pa->p_args.max_size && !(pa->pick_anyway && sa->right_input)) r_b = tar_l+1; else r_b = pr_min - pa->p_args.max_size; if ( pa->pick_right_primer ) { /* We will need a right primer */ length = n-pa->p_args.min_size - r_b + 1; start = r_b; /* Use the primer provided */ if (sa->right_input) { add_one_primer(sa->right_input, &right, &retval->rev, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); /* pick_right_primers(start, length, &right, &retval->rev, pa, sa, dpal_arg_to_use, retval);*/ } /* Pick primers at one position */ else if(sa->force_right_start > -1 || sa->force_right_end > -1) { pick_primers_by_position(sa->force_right_start, sa->force_right_end, &right, &retval->rev, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } /* Or pick all good in the given range */ else { pick_primer_range(start, length, &right, &retval->rev, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } } /* * Return 1 if either the left primer list or the right primer * list is empty or if leftmost left primer and * rightmost right primer do not provide sufficient product size. */ if ((pa->pick_left_primer && 0 == retval->fwd.num_elem) || ((pa->pick_right_primer) && 0 == retval->rev.num_elem)) { return 1; } else if (!((sa->right_input) && (sa->left_input)) && pa->pick_left_primer && pa->pick_right_primer && (right - left) < (pr_min - 1)) { pair_expl->product = 1; pair_expl->considered = 1; return 1; } else return 0; } /* make_detection_primer_lists */ /* * Make complete list of acceptable internal oligos in retval->intl.oligo. * and place the number of valid elements in mid in retval->intl.num_elem. Return 1 if * there are no acceptable internal oligos; otherwise return 0. */ static int make_internal_oligo_list(p3retval *retval, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use) { int ret; int left = 0; /* Use the primer provided */ if ((sa->internal_input) || (pa->primer_task == check_primers)){ ret = add_one_primer(sa->internal_input, &left, &retval->intl, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } else { /* Pick all good in the given range */ /* Use the settings to select a proper range */ int length = strlen(sa->trimmed_seq) - pa->o_args.min_size; int start = pa->o_args.min_size - 1; int left = 0; ret = pick_primer_range(start, length, &left, &retval->intl, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } return ret; } /* make_internal_oligo_list */ /* * Make lists of acceptable left and right primers. After return, the * lists are stored in retval->fwd.oligo and retval->rev.oligo and the * coresponding list sizes are stored in retval->fwd.num_elem and * retval->rev.num_elem. Return 1 if one of lists is empty or if * leftmost left primer and rightmost right primer do not provide * sufficient product size. */ static int make_complete_primer_lists(p3retval *retval, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, const thal_arg_holder *thal_oligo_arg_to_use) { int extreme; int length, start; int n; /* Get the length of the sequence */ PR_ASSERT(INT_MAX > (n=strlen(sa->trimmed_seq))); if (pa->pick_left_primer) { /* We will need a left primer. */ extreme = 0; length = n - pa->p_args.min_size; start = pa->p_args.min_size - 1; /* Pick all good in the given range */ pick_primer_range(start, length, &extreme, &retval->fwd, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } /* if (pa->pick_left_primer) */ if ( pa->pick_right_primer ) { /* We will need a right primer */ extreme = n; length = n - pa->p_args.min_size + 1; start = 0; /* Pick all good in the given range */ pick_primer_range(start, length, &extreme, &retval->rev, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } if ( pa->pick_internal_oligo ) { /* We will need a internal oligo */ length = n - pa->o_args.min_size; start = pa->o_args.min_size - 1; extreme = 0; /* Pick all good in the given range */ pick_primer_range(start, length, &extreme, &retval->intl, pa, sa, dpal_arg_to_use, thal_oligo_arg_to_use, retval); } return 0; } /* make_complete_primer_lists */ /* * Add caller-specified primers to retval->fwd, retval->rev, and/or * retval->intl. The primers do not have to be "legal". It is possible * to add more than one primer to retval->fwd, etc, if the input * primer is found in multiple locations in the template. Similar to * make_complete_primer_lists. */ static int add_primers_to_check(p3retval *retval, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, const thal_arg_holder *thal_oligo_arg_to_use) { int extreme = 0; /* Required when calling add_one_primer but not used in the current function. */ if (sa->left_input) { add_one_primer(sa->left_input, &extreme, &retval->fwd, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } if (sa->right_input) { add_one_primer(sa->right_input, &extreme, &retval->rev, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } if (sa->internal_input) { add_one_primer(sa->internal_input, &extreme, &retval->intl, pa, sa, dpal_arg_to_use, thal_oligo_arg_to_use, retval); } return 0; } /* add_primers_to_check */ /* * Make lists of acceptable left and right primers. After return, the * lists are stored in retval->fwd.oligo and retval->rev.oligo and the * coresponding list sizes are stored in retval->fwd.num_elem and * retval->rev.num_elem. Return 1 if one of lists is empty or if * leftmost left primer and rightmost right primer do not provide * sufficient product size. */ static int pick_sequencing_primer_list(p3retval *retval, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use) { int length, start; int n, rest_accuracy; int primer_nr; /* number of primers we need to pick */ int tar_n; /* counter for the targets */ int step_nr; /* counter to step through the targets */ int sequenced_len; /* bp sequenced in good quality */ int extra_seq; /* bp sequenced additionally on both sides */ /* best location for the 3' end of the fwd primer: */ int pr_position_f; /* best location for the 3' end of the rev primer: */ int pr_position_r; /* Get the length of the sequence */ PR_ASSERT(INT_MAX > (n=strlen(sa->trimmed_seq))); /* For each target needed loop*/ for (tar_n=0; tar_n < sa->tar2.count; tar_n++) { /* Calculate the amount of primers needed */ primer_nr = 1; if ((pa->pick_left_primer) && (pa->pick_right_primer)){ sequenced_len = pa->sequencing.interval; while(sequenced_len < sa->tar2.pairs[tar_n][1]) { primer_nr++; sequenced_len = pa->sequencing.spacing * (primer_nr - 1) + pa->sequencing.interval; } } else { sequenced_len = pa->sequencing.spacing; while(sequenced_len < sa->tar2.pairs[tar_n][1]) { primer_nr++; sequenced_len = pa->sequencing.spacing * primer_nr; } } /* Calculate the overlap on the sides */ extra_seq = (sequenced_len - sa->tar2.pairs[tar_n][1]) / 2; /* Pick primers for each position */ for ( step_nr = 0 ; step_nr < primer_nr ; step_nr++ ) { pr_position_f = sa->tar2.pairs[tar_n][0] - extra_seq + ( pa->sequencing.spacing * step_nr ) - pa->sequencing.lead; if ((pa->pick_left_primer) && (pa->pick_right_primer)) { pr_position_r = sa->tar2.pairs[tar_n][0] - extra_seq + ( pa->sequencing.spacing * step_nr ) + pa->sequencing.interval + pa->sequencing.lead; } else { pr_position_r = sa->tar2.pairs[tar_n][0] - extra_seq + ( pa->sequencing.spacing * (step_nr+1)) + pa->sequencing.lead; } /* Check if calculated positions make sense */ /* position_f cannot be outside included region */ if (pr_position_f < (pa->p_args.min_size -1)) { pr_position_f = pa->p_args.min_size - 1; } if (pr_position_f > (n - pa->p_args.min_size - 1)) { pr_position_f = n - pa->p_args.min_size - 1; /* Actually this should never happen */ pr_append_new_chunk(&retval->warnings, "Calculation error in forward " "sequencing position calculation"); } /* position_r cannot be outside included region */ if (pr_position_r < (pa->p_args.min_size - 1)) { pr_position_r = pa->p_args.min_size - 1; /* Actually this should never happen */ pr_append_new_chunk(&retval->warnings, "Calculation error in reverse " "sequencing position calculation"); } if (pr_position_r > (n - pa->p_args.min_size - 1)) { pr_position_r = n - pa->p_args.min_size - 1; } /* Now all pr_positions are within the sequence */ if (pa->pick_left_primer) { /* Set the start and length for the regions */ start = pr_position_f - pa->sequencing.accuracy; if (start < 0) { rest_accuracy = pr_position_f + 1; start = 0; } else { rest_accuracy = pa->sequencing.accuracy; } length = rest_accuracy + pa->sequencing.accuracy ; if ((start + length) > n) { length = n - start; } /* Pick all good in the given range */ pick_only_best_primer(start, length, &retval->fwd, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } if (pa->pick_right_primer) { start = pr_position_r - pa->sequencing.accuracy; if (start < 0) { rest_accuracy = pr_position_r + 1; start = 0; } else { rest_accuracy = pa->sequencing.accuracy; } length = rest_accuracy + pa->sequencing.accuracy ; if ((start + length) > n) { length = n - start; } /* Pick all good in the given range */ pick_only_best_primer(start, length, &retval->rev, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); } } } /* End of Target Loop */ /* Print an error if not all primers will be printed */ if (retval->fwd.num_elem > pa->num_return || retval->rev.num_elem > pa->num_return) { pr_append_new_chunk( &retval->warnings, "Increase PRIMER_NUM_RETURN to obtain all sequencing primers"); } return 0; } /* pick_sequencing_primer_list */ static void add_oligo_to_oligo_array(oligo_array *oarray, primer_rec orec) { /* Allocate some space for primers if needed */ if (NULL == oarray->oligo) { oarray->storage_size = INITIAL_LIST_LEN; oarray->oligo = (primer_rec *) pr_safe_malloc(sizeof(*oarray->oligo) * oarray->storage_size); } /* If there is no space on the array, allocate new space */ if ((oarray->num_elem + 1) >= oarray->storage_size) { /* TO DO, is +1 really needed? */ oarray->storage_size += (oarray->storage_size >> 1); oarray->oligo = (primer_rec *) pr_safe_realloc(oarray->oligo, oarray->storage_size * sizeof(*oarray->oligo)); } oarray->oligo[oarray->num_elem] = orec; oarray->num_elem++; } /* pick_primer_range picks all primers which have their 3' end in the range * from start to start+length and store only the best *oligo */ static int pick_only_best_primer(const int start, const int length, oligo_array *oligo, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, p3retval *retval) { /* Variables for the loop */ int i, j, primer_size_small, primer_size_large; int n, found_primer; char number[20]; char *p_number = &number[0]; int temp_value; /* Array to store one primer sequences in */ char oligo_seq[MAX_PRIMER_LENGTH+1]; /* Struct to store the primer parameters in */ primer_rec h; primer_rec best; memset(&h, 0, sizeof(primer_rec)); memset(&best, 0, sizeof(primer_rec)); best.quality = 1000.00; found_primer = 0; /* Set n to the length of included region */ PR_ASSERT(INT_MAX > (n=strlen(sa->trimmed_seq))); /* Conditions for primer length */ if (oligo->type == OT_INTL) { primer_size_small=pa->o_args.min_size; primer_size_large=pa->o_args.max_size; } else { primer_size_small=pa->p_args.min_size; primer_size_large=pa->p_args.max_size; } /* Loop over locations in the sequence */ for(i = start + length - 1; i >= start; i--) { oligo_seq[0] = '\0'; /* Loop over possible primer lengths, from min to max */ for (j = primer_size_small; j <= primer_size_large; j++) { /* Set the length of the primer */ h.length = j; /* Set repeat_sim to NULL as indicator that the repeat_sim struct is not initialized. */ h.repeat_sim.score = NULL; /* Figure out positions for left primers and internal oligos */ if (oligo->type != OT_RIGHT) { /* Break if the primer is bigger than the sequence left */ if(i-j < -1) continue; /* Set the start of the primer */ h.start = i - j + 1; /* Put the real primer sequence in oligo_seq */ _pr_substr(sa->trimmed_seq, h.start, j, oligo_seq); } else { /* Figure out positions for reverse primers */ /* Break if the primer is bigger than the sequence left*/ if(i+j > n) continue; /* Set the start of the primer */ h.start = i+j-1; /* Put the real primer sequence in s */ _pr_substr(sa->trimmed_seq, i, j, oligo_seq); } /* Do not force primer3 to use this oligo */ h.must_use = 0; h.overlaps = 0; /* Add it to the considered statistics */ oligo->expl.considered++; /* Calculate all the primer parameters */ calc_and_check_oligo_features(pa, &h, oligo->type, dpal_arg_to_use, thal_arg_to_use, sa, &oligo->expl, retval, oligo_seq); /* If primer has to be used or is OK */ if (OK_OR_MUST_USE(&h)) { /* Calculate the penalty */ h.quality = p_obj_fn(pa, &h, oligo->type); /* Save the primer in the array */ if (h.quality < best.quality) { /* Free memory used by previous best primer. */ free_primer_repeat_sim_score(&best); best = h; found_primer = 1; } else { /* Free memory used by this primer. */ free_primer_repeat_sim_score(&h); } } else { /* Free memory used by this primer. */ free_primer_repeat_sim_score(&h); if (any_5_prime_ol_extension_has_problem(&h)) { /* Break from the inner for loop, because there is no legal longer oligo with the same 3' sequence. */ break; } } } /* j: Loop over possible primer length from min to max */ } /* i: Loop over the sequence */ if (found_primer == 1) { /* Add the best to the array */ add_oligo_to_oligo_array(oligo, best); /* Update statistics with how many primers are good */ oligo->expl.ok = oligo->expl.ok + 1; } else { if (oligo->type == OT_RIGHT) { pr_append_new_chunk(&retval->warnings, "No right primer found in range "); } else { pr_append_new_chunk(&retval->warnings, "No left primer found in range "); } temp_value = start + pa->first_base_index; sprintf(p_number, "%d", temp_value); pr_append(&retval->warnings, p_number); pr_append(&retval->warnings, " - "); temp_value = start + length + pa->first_base_index; sprintf(p_number, "%d", temp_value); pr_append(&retval->warnings, p_number); } if (oligo->num_elem == 0) return 1; else return 0; } /* pick_only_best_primer */ /* pick_primer_range picks all legal primers in the range from start to start+length and stores them in *oligo */ static int pick_primer_range(const int start, const int length, int *extreme, oligo_array *oligo, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, p3retval *retval) { /* Variables for the loop */ int i, j; int primer_size_small, primer_size_large; int pr_min, n; /* Array to store one primer sequences in */ char oligo_seq[MAX_PRIMER_LENGTH+1]; /* Struct to store the primer parameters in */ primer_rec h; memset(&h, 0, sizeof(primer_rec)); /* Set pr_min to the very smallest allowable product size. */ pr_min = INT_MAX; for (i=0; i < pa->num_intervals; i++) if(pa->pr_min[i] < pr_min) pr_min = pa->pr_min[i]; /* Set n to the length of included region */ PR_ASSERT(INT_MAX > (n=strlen(sa->trimmed_seq))); if (oligo->type == OT_INTL) { primer_size_small=pa->o_args.min_size; primer_size_large=pa->o_args.max_size; } else { primer_size_small=pa->p_args.min_size; primer_size_large=pa->p_args.max_size; } /* Loop over locations in the sequence */ for(i = start + length; i >= start; i--) { oligo_seq[0] = '\0'; /* Loop over possible primer lengths, from min to max */ for (j = primer_size_small; j <= primer_size_large; j++) { /* Set the length of the primer */ h.length = j; /* Figure out positions for left primers and internal oligos */ if (oligo->type != OT_RIGHT) { /* Check if the product is of sufficient size */ if (i-j > n-pr_min-1 && retval->output_type == primer_pairs && oligo->type == OT_LEFT) continue; /* Break if the primer is bigger than the sequence left */ if (i-j < -1) break; /* Set the start of the primer */ h.start = i - j + 1; /* Put the real primer sequence in oligo_seq */ _pr_substr(sa->trimmed_seq, h.start, j, oligo_seq); } else { /* Figure out positions for reverse primers */ /* Check if the product is of sufficient size */ if (i+j < pr_min && retval->output_type == primer_pairs) continue; /* Break if the primer is bigger than the sequence left*/ if (i+j > n) break; /* Set the start of the primer */ h.start = i+j-1; /* Put the real primer sequence in s */ _pr_substr(sa->trimmed_seq, i, j, oligo_seq); } /* Do not force primer3 to use this oligo */ h.must_use = 0; h.overlaps = 0; /* Add it to the considered statistics */ oligo->expl.considered++; /* Calculate all the primer parameters */ calc_and_check_oligo_features(pa, &h, oligo->type, dpal_arg_to_use, thal_arg_to_use, sa, &oligo->expl, retval, oligo_seq); /* If primer has to be used or is OK */ if (OK_OR_MUST_USE(&h)) { /* Calculate the penalty */ h.quality = p_obj_fn(pa, &h, oligo->type); /* Save the primer in the array */ add_oligo_to_oligo_array(oligo, h); /* Update the most extreme primer variable */ if (( h.start < *extreme) && (oligo->type != OT_RIGHT)) *extreme = h.start; /* Update the most extreme primer variable */ if ((h.start > *extreme) && (oligo->type == OT_RIGHT)) *extreme = h.start; } else { /* Free memory used by this primer. */ free_primer_repeat_sim_score(&h); if (any_5_prime_ol_extension_has_problem(&h)) { /* Break from the inner for loop, because there is no legal longer oligo with the same 3' sequence. */ break; } } } /* j: Loop over possible primer length from min to max */ } /* i: Loop over the sequence */ /* Update statistics with how many primers are good */ oligo->expl.ok = oligo->num_elem; if (oligo->num_elem == 0) return 1; else return 0; } /* pick_primer_range */ /* add_one_primer finds one primer in the trimmed sequence and stores * it in *oligo The main difference to the general fuction is that it * calculates its length and it will add a primer of any length to the * list */ static int add_one_primer(const char *primer, int *extreme, oligo_array *oligo, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, p3retval *retval) { /* Variables for the loop */ int i, j; int n; /* Array to store one primer sequences in */ char oligo_seq[MAX_PRIMER_LENGTH+1] , test_oligo[MAX_PRIMER_LENGTH+1]; /* Struct to store the primer parameters in */ primer_rec h; memset(&h, 0, sizeof(primer_rec)); /* Copy *primer into test_oligo */ test_oligo[0] = '\0'; if (oligo->type != OT_RIGHT) { strncat(test_oligo, primer, MAX_PRIMER_LENGTH); } else { p3_reverse_complement(primer, test_oligo); } PR_ASSERT(INT_MAX > (n=strlen(sa->trimmed_seq))); /* This time we already know the size of the primer */ j = strlen(primer); /* Loop over the whole sequence */ for(i = strlen(sa->trimmed_seq); i >= 0; i--) { oligo_seq[0] = '\0'; /* Set the length of the primer */ h.length = j; /* Figure out positions for forward primers */ if (oligo->type != OT_RIGHT) { /* Break if the primer is bigger than the sequence left*/ if(i-j < -1) continue; /* Set the start of the primer */ h.start = i - j +1; /* Put the real primer sequence in s */ _pr_substr(sa->trimmed_seq, h.start, j, oligo_seq); } /* Figure out positions for reverse primers */ else { /* Break if the primer is bigger than the sequence left*/ if(i+j>n) continue; /* Set the start of the primer */ h.start=i+j-1; /* Put the real primer sequence in s */ _pr_substr(sa->trimmed_seq, i, j, oligo_seq); } /* Compare the primer with the sequence */ if (strcmp_nocase(test_oligo, oligo_seq)) continue; /* Force primer3 to use this oligo */ h.must_use = (1 && pa->pick_anyway); h.overlaps = 0; /* Add it to the considered statistics */ oligo->expl.considered++; /* Calculate all the primer parameters */ calc_and_check_oligo_features(pa, &h, oligo->type, dpal_arg_to_use, thal_arg_to_use, sa, &oligo->expl, retval, oligo_seq); /* If primer has to be used or is OK */ if (OK_OR_MUST_USE(&h)) { /* Calculate the penalty */ h.quality = p_obj_fn(pa, &h, oligo->type); /* Save the primer in the array */ add_oligo_to_oligo_array(oligo, h); /* Update the most extreme primer variable */ if ((h.start < *extreme) && (oligo->type != OT_RIGHT)) *extreme = h.start; /* Update the most extreme primer variable */ if ((h.start > *extreme) && (oligo->type == OT_RIGHT)) *extreme = h.start; } else { /* Free memory used by this primer. */ free_primer_repeat_sim_score(&h); } } /* i: Loop over the sequence */ /* Update array with how many primers are good */ /* Update statistics with how many primers are good */ oligo->expl.ok = oligo->num_elem; if (oligo->num_elem == 0) return 1; else { if (oligo->num_elem > 1) { pr_append_new_chunk(&retval->warnings, "More than one position in template for input oligo "); pr_append(&retval->warnings, primer); } return 0; /* Success */ } } /* add_one_primer finds one primer in the trimmed sequence and stores * it in *oligo The main difference to the general fuction is that it * calculates its length and it will add a primer of any length to the * list */ static int add_one_primer_by_position(int start, int length, int *extreme, oligo_array *oligo, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, p3retval *retval) { /* Variables for the loop */ int i; int n, found_primer; /* Array to store one primer sequences in */ char oligo_seq[MAX_PRIMER_LENGTH+1]; /* Struct to store the primer parameters in */ primer_rec h; memset(&h, 0, sizeof(primer_rec)); /* Retun 1 for no primer found */ found_primer = 1; PR_ASSERT(INT_MAX > (n=strlen(sa->trimmed_seq))); /* Just to be sure */ if (start < 0) { return 1; } if (start >= n) { return 1; } if (oligo->type != OT_RIGHT) { if ((start + length) > n) { return 1; } } else { if ((start - length + 1) < 0) { return 1; } } oligo_seq[0] = '\0'; /* Set the length of the primer */ h.length = length; /* Figure out positions for forward primers */ if (oligo->type != OT_RIGHT) { /* Set the start of the primer */ h.start = start; /* Put the real primer sequence in s */ _pr_substr(sa->trimmed_seq, h.start, length, oligo_seq); } /* Figure out positions for reverse primers */ else { i = start - length + 1; /* Set the start of the primer */ h.start = start; /* Put the real primer sequence in s */ _pr_substr(sa->trimmed_seq, i, length, oligo_seq); } /* Force primer3 to use this oligo */ h.must_use = (1 && pa->pick_anyway); h.overlaps = 0; /* Add it to the considered statistics */ oligo->expl.considered++; /* Calculate all the primer parameters */ calc_and_check_oligo_features(pa, &h, oligo->type, dpal_arg_to_use, thal_arg_to_use, sa, &oligo->expl, retval, oligo_seq); /* If primer has to be used or is OK */ if (OK_OR_MUST_USE(&h)) { /* Calculate the penalty */ h.quality = p_obj_fn(pa, &h, oligo->type); /* Save the primer in the array */ add_oligo_to_oligo_array(oligo, h); found_primer = 0; /* Update the most extreme primer variable */ if (( h.start < *extreme) && (oligo->type != OT_RIGHT)) *extreme = h.start; /* Update the most extreme primer variable */ if (( h.start > *extreme) && (oligo->type == OT_RIGHT)) *extreme = h.start; /* Update the number of primers */ } else { /* Free memory used by this primer. */ free_primer_repeat_sim_score(&h); } /* Update array with how many primers are good */ /* Update statistics with how many primers are good */ oligo->expl.ok = oligo->num_elem; /* return 0 for success */ return found_primer; } static int pick_primers_by_position(const int start, const int end, int *extreme, oligo_array *oligo, const p3_global_settings *pa, const seq_args *sa, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, p3retval *retval) { int found_primer, length, j, ret, new_start; found_primer = 1; ret = 1; if(start > -1 && end > -1) { if (oligo->type != OT_RIGHT) { length = end - start + 1; } else { length = start - end + 1; } found_primer = add_one_primer_by_position(start, length, extreme, oligo, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); return found_primer; } else if (start > -1) { /* Loop over possible primer lengths, from min to max */ ret = 0; for (j = pa->p_args.min_size; j <= pa->p_args.max_size; j++) { ret += add_one_primer_by_position(start, j, extreme, oligo, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); if (ret == 0) { found_primer = 0; } } return found_primer; } else if (end > -1) { /* Loop over possible primer lengths, from min to max */ ret = 0; for (j = pa->p_args.min_size; j <= pa->p_args.max_size; j++) { if (oligo->type != OT_RIGHT) { new_start = end - j + 1; } else { new_start = end + j - 1; } ret += add_one_primer_by_position(new_start, j, extreme, oligo, pa, sa, dpal_arg_to_use, thal_arg_to_use, retval); if (ret == 0) { found_primer = 0; } } return found_primer; } else { /* Actually this should never happen */ pr_append_new_chunk(&retval->warnings, "Calculation error in forced primer position calculation"); return 1; } } /* * Compute various characteristics of the oligo, and determine * if it is acceptable. */ #define OUTSIDE_START_WT 30.0 #define INSIDE_START_WT 20.0 #define INSIDE_STOP_WT 100.0 #define OUTSIDE_STOP_WT 0.5 static void calc_and_check_oligo_features(const p3_global_settings *pa, primer_rec *h, oligo_type otype, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, const seq_args *sa, oligo_stats *stats, p3retval *retval, /* This is 5'->3' on the template sequence: */ const char *input_oligo_seq ) { int i, j, k, for_i, gc_count; int three_prime_pos; /* position of 3' base of oligo */ oligo_type l = otype; int poly_x, max_poly_x; int must_use = h->must_use; int three_conditions = (must_use || pa->file_flag || retval->output_type == primer_list); const char *seq = sa->trimmed_seq; const thal_args *thal_args_for_template_mispriming = use_end_for_th_template_mispriming ? thal_arg_to_use->end1 : thal_arg_to_use->any; char s1_rev[MAX_PRIMER_LENGTH+1]; const char *oligo_seq; const char *revc_oligo_seq; const args_for_one_oligo_or_primer *po_args; /* Initialize slots in h */ initialize_op(h); h->overlaps = 0; /* Set repeat_sim to NULL as indicator that the repeat_sim struct is not initialized. */ h->repeat_sim.score = NULL; h->gc_content = h->num_ns = 0; h->overlaps_overlap_position = 0; h->template_mispriming = h->template_mispriming_r = ALIGN_SCORE_UNDEF; h->template_mispriming_ok = 0; PR_ASSERT(OT_LEFT == l || OT_RIGHT == l || OT_INTL == l); p3_reverse_complement(input_oligo_seq, s1_rev); if (OT_RIGHT == l) { oligo_seq = s1_rev; revc_oligo_seq = input_oligo_seq; } else { oligo_seq = input_oligo_seq; revc_oligo_seq = s1_rev; } if (OT_INTL == l) { po_args = &pa->o_args; } else { po_args = &pa->p_args; } /* Set j and k, and sanity check */ if (OT_LEFT == otype || OT_INTL == otype) { j = h->start; three_prime_pos = k = j+h->length-1; } else { three_prime_pos = j = h->start-h->length+1; k = h->start; } PR_ASSERT(k >= 0); PR_ASSERT(k < TRIMMED_SEQ_LEN(sa)); if ((otype == OT_LEFT) && !PR_START_CODON_POS_IS_NULL(sa) /* Make sure the primer would amplify at least part of the ORF. */ && (0 != (h->start - sa->start_codon_pos) % 3 || h->start <= retval->upstream_stop_codon || (retval->stop_codon_pos != -1 && h->start >= retval->stop_codon_pos))) { stats->no_orf++; op_set_does_not_amplify_orf(h); if (!pa->pick_anyway) return; } /* edited by T. Koressaar for lowercase masking */ if(pa->lowercase_masking == 1) { if (is_lowercase_masked(three_prime_pos, sa->trimmed_orig_seq, h, stats)) { if (!must_use) return; } } /* end T. Koressar's changes */ /* edited by A. Untergasser for forcing sequence use */ if ((po_args->must_match_five_prime != NULL) or (po_args->must_match_three_prime != NULL)) { if (primer_must_match(pa, h, stats, oligo_seq, po_args->must_match_three_prime, po_args->must_match_five_prime)) { if (!must_use) { op_set_must_match_err(h); return; } } } /* end A. Untergasser's changes */ gc_and_n_content(j, k-j+1, sa->trimmed_seq, h); if (h->num_ns > po_args->num_ns_accepted) { op_set_too_many_ns(h); stats->ns++; if (!must_use) return; } /* Upstream error checking has ensured that we use non-default position penalties only when there is 0 or 1 target. */ PR_ASSERT(sa->tar2.count <= 1 || _PR_DEFAULT_POSITION_PENALTIES(pa)); if (pa->primer_task == pick_sequencing_primers) { h->position_penalty = 0.0; } else if (l != OT_INTL && _PR_DEFAULT_POSITION_PENALTIES(pa) && oligo_overlaps_interval(j, k-j+1, sa->tar2.pairs, sa->tar2.count)) { h->position_penalty = 0.0; bf_set_infinite_pos_penalty(h,1); bf_set_overlaps_target(h,1); } else if (l != OT_INTL && !_PR_DEFAULT_POSITION_PENALTIES(pa) && 1 == sa->tar2.count) { compute_position_penalty(pa, sa, h, l); if (bf_get_infinite_pos_penalty(h)) { bf_set_overlaps_target(h,1); } } else { h->position_penalty = 0.0; bf_set_infinite_pos_penalty(h,0); } if (!PR_START_CODON_POS_IS_NULL(sa)) { if (OT_LEFT == l) { if (sa->start_codon_pos > h->start) h->position_penalty = (sa->start_codon_pos - h->start) * OUTSIDE_START_WT; else h->position_penalty = (h->start - sa->start_codon_pos) * INSIDE_START_WT; } else if (OT_RIGHT == l) { if (-1 == retval->stop_codon_pos) { h->position_penalty = (TRIMMED_SEQ_LEN(sa) - h->start - 1) * INSIDE_STOP_WT; } else if (retval->stop_codon_pos < h->start) { h->position_penalty = (h->start - retval->stop_codon_pos) * OUTSIDE_STOP_WT; } else { h->position_penalty = (retval->stop_codon_pos - h->start) * INSIDE_STOP_WT; } } } /* TO DO Simplify logic here */ if (l != OT_INTL && oligo_overlaps_interval(j, k-j+1, sa->excl2.pairs, sa->excl2.count)) bf_set_overlaps_excl_region(h,1); if (l == OT_INTL && oligo_overlaps_interval(j, k-j+1, sa->excl_internal2.pairs, sa->excl_internal2.count)) bf_set_overlaps_excl_region(h,1); if(l != OT_INTL && bf_get_overlaps_target(h)) { op_set_overlaps_target(h); stats->target++; if (!must_use) return; } if(bf_get_overlaps_excl_region(h)){ op_set_overlaps_excluded_region(h); stats->excluded++; if (!must_use) return; } /* Check if the oligo is included in any ok region */ if ((l == OT_LEFT) && (sa->ok_regions.count > 0) && (!sa->ok_regions.any_left)) { int included = 0; for (i=0; iok_regions.count; i++) { if ((j >= sa->ok_regions.left_pairs[i][0]) && (k <= sa->ok_regions.left_pairs[i][0] + sa->ok_regions.left_pairs[i][1] - 1)) { included = 1; break; } } if (!included) { op_set_not_in_any_ok_region(h); stats->not_in_any_left_ok_region++; if (!must_use) return; } } if ((l == OT_RIGHT) && (sa->ok_regions.count > 0) && (!sa->ok_regions.any_right)) { int included = 0; for (i=0; iok_regions.count; i++) { if ((j >= sa->ok_regions.right_pairs[i][0]) && (k <= sa->ok_regions.right_pairs[i][0] + sa->ok_regions.right_pairs[i][1] - 1)) { included = 1; break; } } if (!included) { op_set_not_in_any_ok_region(h); stats->not_in_any_right_ok_region++; if (!must_use) return; } } if (h->gc_content < po_args->min_gc) { op_set_low_gc_content(h); stats->gc++; if (!must_use) return; } else if (h->gc_content > po_args->max_gc) { op_set_high_gc_content(h); stats->gc++; if (!must_use) return; } if (OT_LEFT == l || OT_RIGHT == l) { /* gc_clamp is applicable only to primers (as opposed to primers and hybridzations oligos. */ for (i = 0; i < pa->gc_clamp; i++) { /* We want to look at the 3' end of the oligo being assessed, so we look in the 5' end of its reverse-complement */ if (revc_oligo_seq[i] != 'G' && revc_oligo_seq[i] != 'C') { op_set_no_gc_glamp(h); stats->gc_clamp++; if (!must_use) return; else break; } } } #if 0 /* Old code, works */ if(pa->gc_clamp != 0){ if(OT_LEFT == l) { for(i=k-pa->gc_clamp+1; i<= k; i++)if(seq[i] !='G'&&seq[i] !='C'){ op_set_no_gc_glamp(h); stats->gc_clamp++; if (!must_use) return; else break; } } if(OT_RIGHT == l){ for(i=j; igc_clamp; i++)if(seq[i] != 'G' && seq[i] != 'C'){ op_set_no_gc_glamp(h); stats->gc_clamp++; if (!must_use) return; else break; } } } #endif if (pa->max_end_gc < 5 && (OT_LEFT == l || OT_RIGHT == l)) { /* The CGs are only counted in the END of primers (as opposed to primers and hybridzations oligos. */ gc_count=0; for (i = 0; i < 5; i++) { /* We want to look at the 3' end of the oligo being assessed, so we look in the 5' end of its reverse-complement */ if (revc_oligo_seq[i] == 'G' || revc_oligo_seq[i] == 'C') { gc_count++; } } if (gc_count > pa->max_end_gc) { op_set_too_many_gc_at_end(h); stats->gc_end_high++; if (!must_use) return; } } /* Tentative interface for generic oligo testing function */ if (!sequence_quality_is_ok(pa, h, l, sa, j, k, stats, po_args) && !must_use) return; max_poly_x = po_args->max_poly_x; if (max_poly_x > 0) { poly_x = 1; for(i=j+1;i<=k;i++){ if(seq[i] == seq[i-1]||seq[i] == 'N'){ poly_x++; if(poly_x > max_poly_x){ op_set_high_poly_x(h); stats->poly_x++; if (!must_use) return; else break; } } else poly_x = 1; } } h->temp /* Oligo/primer melting temperature */ = seqtm(oligo_seq, po_args->dna_conc, po_args->salt_conc, po_args->divalent_conc, po_args->dntp_conc, MAX_NN_TM_LENGTH, pa->tm_santalucia, pa->salt_corrections); if (h->temp < po_args->min_tm) { op_set_low_tm(h); stats->temp_min++; if (!must_use) return; } if (h->temp > po_args->max_tm) { op_set_high_tm(h); stats->temp_max++; if (!must_use) return; } /* End stability is applicable only to primers (not to oligos) */ if (OT_LEFT == l || OT_RIGHT == l) { if ((h->end_stability = end_oligodg(oligo_seq, 5, pa->tm_santalucia)) > pa->max_end_stability) { /* Must not be called on a hybridization probe / internal oligo: */ op_set_high_end_stability(h); stats->stability++; if (!must_use) return; } } if ((must_use || pa->file_flag || retval->output_type == primer_list || po_args->weights.compl_any || po_args->weights.compl_end) && pa->thermodynamic_oligo_alignment==0) { oligo_compl(h, po_args, stats, dpal_arg_to_use, oligo_seq, revc_oligo_seq); if ((!(p3_ol_is_uninitialized(h))) && !must_use) { PR_ASSERT(!p3_ol_is_ok(h)); return; } } else { /* Thermodynamical approach: for primers only */ if ((must_use || pa->file_flag || retval->output_type == primer_list || po_args->weights.compl_any_th || po_args->weights.compl_end_th) && pa->thermodynamic_oligo_alignment==1 ) { oligo_compl_thermod(h, po_args, stats, thal_arg_to_use, oligo_seq, oligo_seq); /* input_oligo_seq, input_oligo_seq);*/ /* oligo_seq, revc_oligo_seq); */ if ((!(p3_ol_is_uninitialized(h))) && !must_use) { PR_ASSERT(!p3_ol_is_ok(h)); return; } } else { h->self_any = h->self_end = ALIGN_SCORE_UNDEF; } } if ((three_conditions || po_args->weights.hairpin_th #if 0 || po_args->weights.compl_any_th /* Triinu, is this needed? */ || po_args->weights.compl_end_th /* Triinu, is this needed? */ #endif ) && pa->thermodynamic_oligo_alignment==1 ) { oligo_hairpin(h, po_args, stats, thal_arg_to_use, /* input_oligo_seq);*/ oligo_seq); if ((!(p3_ol_is_uninitialized(h))) && !must_use) { PR_ASSERT(!p3_ol_is_ok(h)); return; } } else { /* This will get calculated later if necessary, in characterize_pair. */ h->hairpin_th = ALIGN_SCORE_UNDEF; } /* end of thermod. approach */ #if 0 /* FIX ME FIXME */ if (((must_use || pa->file_flag || retval->output_type == primer_list || po_args->weights.repeat_sim || ((OT_RIGHT == l || OT_LEFT == l) && pa->p_args.weights.template_mispriming)) && pa->thermodynamic_oligo_alignment==0) || (pa->thermodynamic_oligo_alignment==1 && po_args->weights.repeat_sim)) { oligo_repeat_library_mispriming(h, pa, sa, l, stats, dpal_arg_to_use); if (pa->thermodynamic_template_alignment==0 && OK_OR_MUST_USE(h)) { oligo_template_mispriming(h, pa, sa, l, stats, dpal_arg_to_use->local_end, thal_args_for_template_mispriming); } } if ((must_use || pa->file_flag || retval->output_type == primer_list || ((OT_RIGHT == l || OT_LEFT == l) && pa->p_args.weights.template_mispriming_th)) && pa->thermodynamic_template_alignment==1) { oligo_template_mispriming(h, pa, sa, l, stats, dpal_arg_to_use->local_end, thal_args_for_template_mispriming); } /* END FIX ME FIXME */ #else if (three_conditions || po_args->weights.repeat_sim) { oligo_repeat_library_mispriming(h, pa, sa, l, stats, dpal_arg_to_use); } if (!OK_OR_MUST_USE(h)) return; if (three_conditions || ( /* Do we need template mispriming for the penalty function? */ (OT_RIGHT == l || OT_LEFT == l) && ( (pa->p_args.weights.template_mispriming && !pa->thermodynamic_template_alignment) || (pa->p_args.weights.template_mispriming_th && pa->thermodynamic_template_alignment) ) ) ) { if (OK_OR_MUST_USE(h)) { oligo_template_mispriming(h, pa, sa, l, stats, dpal_arg_to_use->local_end, thal_args_for_template_mispriming); } } #endif if (h->length > po_args->max_size ) { op_set_too_long(h); stats->size_max ++; if (!must_use) return; } if (h->length < po_args->min_size ) { op_set_too_short(h); stats->size_min ++; if (!must_use) return; } for (for_i=0; for_i < sa->primer_overlap_junctions_count; for_i++) { if (OT_LEFT == l && ((h->start + pa->min_5_prime_overlap_of_junction - 1) <= sa->primer_overlap_junctions[for_i]) && ((h->start + h->length - pa->min_3_prime_overlap_of_junction)) > sa->primer_overlap_junctions[for_i]) { h->overlaps_overlap_position = 1; /* no need to continue checking */ break; } if (OT_RIGHT == l && ((h->start - h->length + pa->min_3_prime_overlap_of_junction) <= sa->primer_overlap_junctions[for_i]) && ((h->start - pa->min_5_prime_overlap_of_junction + 1)) > sa->primer_overlap_junctions[for_i]) { h->overlaps_overlap_position = 1; /* no need to continue checking */ break; } } /* FIX ME FIXME Steve, is this really needed? */ op_set_completely_written(h); } /* calc_and_check_oligo_features */ #undef OUTSIDE_START_WT #undef INSIDE_START_WT #undef INSIDE_STOP_WT #undef OUTSIDE_STOP_WT /* Calculate the minimum sequence quality and the minimum sequence quality of the 3' end of a primer or oligo. Set h->seq_quality and h->seq_end_quality with these values. Return 1 (== ok) if sa->quality is undefined. Otherwise, return 1 (ok) if h->seq_quality and h->seq_end_quality are within range, or else return 0. */ static int sequence_quality_is_ok(const p3_global_settings *pa, primer_rec *h, oligo_type l, const seq_args *sa, int j, int k, oligo_stats *global_oligo_stats, const args_for_one_oligo_or_primer *po_args) { int i, min_q, min_q_end, m, q; int retval = 1; if (NULL == sa->quality) { h->seq_end_quality = h->seq_quality = pa->quality_range_max; return 1; } q = pa->quality_range_max; min_q = po_args->min_quality; if (OT_LEFT == l || OT_RIGHT == l) { min_q_end = po_args->min_end_quality; } else { min_q_end = min_q; } if (OT_LEFT == l || OT_INTL == l) { for(i = k-4; i <= k; i++) { if(i < j) continue; m = sa->quality[i + sa->incl_s]; if (m < q) q = m; } min_q_end = q; for(i = j; i<=k-5; i++) { m = sa->quality[i + sa->incl_s]; if (m < q) q = m; } min_q = q; } else if (OT_RIGHT == l) { for(i = j; i < j+5; i++) { if(i > k) break; m = sa->quality[i + sa->incl_s]; if (m < q) q = m; } min_q_end = q; for(i = j+5; i <= k; i++) { m = sa->quality[i + sa->incl_s]; if (m < q) q = m; } min_q = q; } else { PR_ASSERT(0); /* Programming error. */ } h->seq_quality = min_q; h->seq_end_quality = min_q_end; if (h->seq_quality < po_args->min_quality) { op_set_low_sequence_quality(h); global_oligo_stats->seq_quality++; retval = 0; return retval; } if (OT_LEFT == l || OT_RIGHT == l) { if (h->seq_end_quality < po_args->min_end_quality) { op_set_low_end_sequence_quality(h); global_oligo_stats->seq_quality++; retval = 0; } } return retval; } /* * Set h->gc_content to the GC % of the 'len' bases starting at 'start' in * 'sequence' (excluding 'N's). Set h->num_ns to the number of 'N's in that * subsequence. */ static void gc_and_n_content(int start, int len, const char *sequence, primer_rec *h) { const char* p = &sequence[start]; const char* stop = p + len; int num_gc = 0, num_gcat = 0, num_n = 0; while (p < stop) { if ('N' == *p) num_n++; else { num_gcat++; if ('C' == *p || 'G' == *p) num_gc++; } p++; } h->num_ns = num_n; if (0 == num_gcat) h->gc_content= 0.0; else h->gc_content = 100.0 * ((double)num_gc)/num_gcat; } static int oligo_overlaps_interval(int start, int len, const interval_array_t intervals, int num_intervals) { int i; int last = start + len - 1; for (i = 0; i < num_intervals; i++) if (!(last < intervals[i][0] || start > (intervals[i][0] + intervals[i][1] - 1))) return 1; return 0; } /* Calculate the part of the objective function due to one primer. */ static double p_obj_fn(const p3_global_settings *pa, primer_rec *h, int j) { double sum; sum = 0; if (j == OT_LEFT || j == OT_RIGHT) { if(pa->p_args.weights.temp_gt && h->temp > pa->p_args.opt_tm) sum += pa->p_args.weights.temp_gt * (h->temp - pa->p_args.opt_tm); if (pa->p_args.weights.temp_lt && h->temp < pa->p_args.opt_tm) sum += pa->p_args.weights.temp_lt * (pa->p_args.opt_tm - h->temp); if (pa->p_args.weights.gc_content_gt && h->gc_content > pa->p_args.opt_gc_content) sum += pa->p_args.weights.gc_content_gt * (h->gc_content - pa->p_args.opt_gc_content); if (pa->p_args.weights.gc_content_lt && h->gc_content < pa->p_args.opt_gc_content) sum += pa->p_args.weights.gc_content_lt * (pa->p_args.opt_gc_content - h->gc_content); if (pa->p_args.weights.length_lt && h->length < pa->p_args.opt_size) sum += pa->p_args.weights.length_lt * (pa->p_args.opt_size - h->length); if (pa->p_args.weights.length_gt && h->length > pa->p_args.opt_size) sum += pa->p_args.weights.length_gt * (h->length - pa->p_args.opt_size); /* BEGIN: secondary structures */ if (pa->thermodynamic_oligo_alignment==0) { if (pa->p_args.weights.compl_any) sum += pa->p_args.weights.compl_any * h->self_any; if (pa->p_args.weights.compl_end) sum += pa->p_args.weights.compl_end * h->self_end; } else if (pa->thermodynamic_oligo_alignment==1) { if (pa->p_args.weights.compl_any_th) { if ((h->temp - pa->p_args.weights.temp_cutoff) <= h->self_any) sum += pa->p_args.weights.compl_any_th * (h->self_any - (h->temp - pa->p_args.weights.temp_cutoff - 1.0)); /* -1.0 is added for the case where == */ else sum += pa->p_args.weights.compl_any_th * (1/(h->temp - pa->p_args.weights.temp_cutoff + 1.0 - h->self_any)); } if (pa->p_args.weights.compl_end_th) { if ((h->temp - pa->p_args.weights.temp_cutoff) <= h->self_end) sum += pa->p_args.weights.compl_end_th * (h->self_end - (h->temp - pa->p_args.weights.temp_cutoff - 1.0)); else sum += pa->p_args.weights.compl_end_th * (1/(h->temp - pa->p_args.weights.temp_cutoff + 1.0 - h->self_end)); } if (pa->p_args.weights.hairpin_th) { if ((h->temp - pa->p_args.weights.temp_cutoff) <= h->hairpin_th) sum += pa->p_args.weights.hairpin_th * (h->hairpin_th - (h->temp - pa->p_args.weights.temp_cutoff - 1.0)); else sum += pa->p_args.weights.hairpin_th * (1/(h->temp - pa->p_args.weights.temp_cutoff + 1.0 - h->hairpin_th)); } } else { PR_ASSERT(0) /* Programming error, illegal value for pa->thermodynamic_oligo_alignment */ } /* END: secondary structures */ if (pa->p_args.weights.num_ns) sum += pa->p_args.weights.num_ns * h->num_ns; if(pa->p_args.weights.repeat_sim) sum += pa->p_args.weights.repeat_sim * h->repeat_sim.score[h->repeat_sim.max]; if (!bf_get_overlaps_target(h)) { /* We might be evaluating p_obj_fn with h->target if the client supplied 'pick_anyway' and specified a primer or oligo. */ PR_ASSERT(!(bf_get_infinite_pos_penalty(h))); if(pa->p_args.weights.pos_penalty) sum += pa->p_args.weights.pos_penalty * h->position_penalty; } if(pa->p_args.weights.end_stability) sum += pa->p_args.weights.end_stability * h->end_stability; /* FIX ME QUALITY WT Change here */ if(pa->p_args.weights.seq_quality) sum += pa->p_args.weights.seq_quality * (pa->quality_range_max - h->seq_quality); /* Look for end seq quality */ if (pa->p_args.weights.template_mispriming && pa->thermodynamic_template_alignment==0) { PR_ASSERT(oligo_max_template_mispriming(h) != ALIGN_SCORE_UNDEF); sum += pa->p_args.weights.template_mispriming * oligo_max_template_mispriming(h); } if (pa->p_args.weights.template_mispriming_th && pa->thermodynamic_template_alignment==1) { PR_ASSERT(oligo_max_template_mispriming_thermod(h) != ALIGN_SCORE_UNDEF); if((h->temp - pa->p_args.weights.temp_cutoff) <= oligo_max_template_mispriming_thermod(h)) sum += pa->p_args.weights.template_mispriming_th * (oligo_max_template_mispriming_thermod(h) - (h->temp - pa->p_args.weights.temp_cutoff - 1.0)); if((h->temp - pa->p_args.weights.temp_cutoff) > oligo_max_template_mispriming_thermod(h)) sum += pa->p_args.weights.template_mispriming_th * (1/(h->temp - pa->p_args.weights.temp_cutoff + 1.0 - oligo_max_template_mispriming_thermod(h))); } return sum; } else if (j == OT_INTL) { if(pa->o_args.weights.temp_gt && h->temp > pa->o_args.opt_tm) sum += pa->o_args.weights.temp_gt * (h->temp - pa->o_args.opt_tm); if(pa->o_args.weights.temp_lt && h->temp < pa->o_args.opt_tm) sum += pa->o_args.weights.temp_lt * (pa->o_args.opt_tm - h->temp); if(pa->o_args.weights.gc_content_gt && h->gc_content > pa->o_args.opt_gc_content) sum += pa->o_args.weights.gc_content_gt * (h->gc_content - pa->o_args.opt_gc_content); if(pa->o_args.weights.gc_content_lt && h->gc_content < pa->o_args.opt_gc_content) sum += pa->o_args.weights.gc_content_lt * (pa->o_args.opt_gc_content - h->gc_content); if(pa->o_args.weights.length_lt && h->length < pa->o_args.opt_size) sum += pa->o_args.weights.length_lt * (pa->o_args.opt_size - h->length); if(pa->o_args.weights.length_gt && h->length > pa->o_args.opt_size) sum += pa->o_args.weights.length_gt * (h->length - pa->o_args.opt_size); if(pa->o_args.weights.compl_any && pa->thermodynamic_oligo_alignment==0) sum += pa->o_args.weights.compl_any * h->self_any; if(pa->o_args.weights.compl_end && pa->thermodynamic_oligo_alignment==0) sum += pa->o_args.weights.compl_end * h->self_end; /* begin thermodynamical approach */ if (pa->thermodynamic_oligo_alignment==1) { if (pa->o_args.weights.compl_any_th) { if ((h->temp - pa->o_args.weights.temp_cutoff) <= h->self_any) sum += pa->o_args.weights.compl_any_th * (h->self_any - (h->temp - pa->o_args.weights.temp_cutoff - 1.0)); /* -1.0 is added for the case where == */ else sum += pa->o_args.weights.compl_any_th * (1/(h->temp - pa->o_args.weights.temp_cutoff + 1.0 - h->self_any)); } if (pa->o_args.weights.compl_end_th) { if ((h->temp - pa->o_args.weights.temp_cutoff) <= h->self_end) sum += pa->o_args.weights.compl_end_th * (h->self_end - (h->temp - pa->o_args.weights.temp_cutoff - 1.0)); else sum += pa->o_args.weights.compl_end_th * (1/(h->temp - pa->o_args.weights.temp_cutoff + 1.0 - h->self_end)); } if (pa->o_args.weights.hairpin_th) { if ((h->temp - pa->o_args.weights.temp_cutoff) <= h->hairpin_th) sum += pa->o_args.weights.hairpin_th * (h->hairpin_th - (h->temp - pa->o_args.weights.temp_cutoff - 1.0)); else sum += pa->o_args.weights.hairpin_th * (1/(h->temp - pa->o_args.weights.temp_cutoff + 1.0 - h->hairpin_th)); } } /* end thermodynamical approach */ if(pa->o_args.weights.num_ns) sum += pa->o_args.weights.num_ns * h->num_ns; if(pa->o_args.weights.repeat_sim) sum += pa->o_args.weights.repeat_sim * h->repeat_sim.score[h->repeat_sim.max]; /* FIX ME QUALITY WT */ if(pa->o_args.weights.seq_quality) sum += pa->o_args.weights.seq_quality * (pa->quality_range_max - h->seq_quality); return sum; } else { PR_ASSERT(0); /* Programmig error. */ } } /* Return max of h->template_mispriming and h->template_mispriming_r (max template mispriming on either strand). */ double oligo_max_template_mispriming(const primer_rec *h) { return h->template_mispriming > h->template_mispriming_r ? h->template_mispriming : h->template_mispriming_r; } double oligo_max_template_mispriming_thermod(const primer_rec *h) { return h->template_mispriming > h->template_mispriming_r ? h->template_mispriming : h->template_mispriming_r; } /* Sort a given primer array by penalty */ static void sort_primer_array(oligo_array *oligo) { qsort(&oligo->oligo[0], oligo->num_elem, sizeof(*oligo->oligo), primer_rec_comp); } /* Compare function for sorting primer records. */ static int primer_rec_comp(const void *x1, const void *x2) { const primer_rec *a1 = (const primer_rec *) x1; const primer_rec *a2 = (const primer_rec *) x2; if(a1->quality < a2->quality) return -1; if (a1->quality > a2->quality) return 1; /* * We want primer_rec_comp to always return a non-0 result, because * different implementations of qsort differ in how they treat "equal" * elements, making it difficult to compare test output on different * systems. */ if(a1->start > a2->start) return -1; if(a1->start < a2->start) return 1; if(a1->length < a2->length) return -1; return 1; } /* Compare function for sorting primer records. */ static int compare_primer_pair(const void *x1, const void *x2) { const primer_pair *a1 = (const primer_pair *) x1; const primer_pair *a2 = (const primer_pair *) x2; const double epsilon = 1e-6; int y1, y2; if ((a1->pair_quality + epsilon) < a2->pair_quality) return -1; if (a1->pair_quality > (a2->pair_quality + epsilon)) return 1; /* * The following statements ensure that we get a stable order that * is the same on all systems. */ y1 = a1->left->start; y2 = a2->left->start; if (y1 > y2) return -1; /* prefer left primers to the right. */ if (y1 < y2) return 1; y1 = a1->right->start; y2 = a2->right->start; if (y1 < y2) return -1; /* prefer right primers to the left. */ if (y1 > y2) return 1; y1 = a1->left->length; y2 = a2->left->length; if (y1 < y2) return -1; /* prefer shorter primers. */ if (y1 > y2) return 1; y1 = a1->right->length; y2 = a2->right->length; if (y1 < y2) return -1; /* prefer shorter primers. */ if (y1 > y2) return 1; return 0; } /* * Defines parameter values for given primer pair. Returns PAIR_OK if the pair is * acceptable; PAIR_FAILED otherwise. This function sets the * various elements of *ppair, and also calculates some key EXPENSIVE * parameters for individual primers if they have not been set yet. */ static int characterize_pair(p3retval *retval, const p3_global_settings *pa, const seq_args *sa, int m, int n, int int_num, primer_pair *ppair, const dpal_arg_holder *dpal_arg_to_use, const thal_arg_holder *thal_arg_to_use, int update_stats) { char s1[MAX_PRIMER_LENGTH+1], s2[MAX_PRIMER_LENGTH+1], s1_rev[MAX_PRIMER_LENGTH+1], s2_rev[MAX_PRIMER_LENGTH+1]; double compl_end; pair_stats *pair_expl = &retval->best_pairs.expl; int must_use = 0; double min_oligo_tm; int i; const thal_args *thal_args_for_template_mispriming = use_end_for_th_template_mispriming ? thal_arg_to_use->end1 : thal_arg_to_use->any; memset(ppair, 0, sizeof(*ppair)); ppair->left = &retval->fwd.oligo[m]; ppair->right = &retval->rev.oligo[n]; ppair->product_size = retval->rev.oligo[n].start - retval->fwd.oligo[m].start+1; ppair->target = 0; ppair->compl_any = ppair->compl_end = 0; if (update_stats) { pair_expl->considered++; } if (pa->primer_task == check_primers) { must_use = 1; } /* We must use the pair if the caller specifed both the left and the right primer. */ if (ppair->left->must_use != 0 && ppair->right->must_use != 0) { /* But if caller specified primers are in reversed order we cannot analyze them as a pair. */ if (ppair->product_size < 1) { pair_expl->reversed++; return PAIR_FAILED; } must_use = 1; } ppair->must_use = must_use; if (sa->tar2.count > 0) { if (pair_spans_target(ppair, sa)) { ppair->target = 1; } else { ppair->target = -1; if (update_stats) { pair_expl->target++; } if (!must_use) return PAIR_FAILED; } } /* ============================================================= */ /* Check that the pair is included in one of the specified ok regions */ if ((sa->ok_regions.count > 0) && (!sa->ok_regions.any_pair)) { int included = 0; int l_start = ppair->left->start, l_end = ppair->left->start + ppair->left->length - 1; int r_start = ppair->right->start - ppair->right->length + 1, r_end = ppair->right->start; for (i=0; iok_regions.count; i++) { if (sa->ok_regions.left_pairs[i][0] == -1) { /* any left primer, check right primer */ if ((r_start >= sa->ok_regions.right_pairs[i][0]) && (r_end <= sa->ok_regions.right_pairs[i][0] + sa->ok_regions.right_pairs[i][1] - 1)) { included = 1; break; } } else if (sa->ok_regions.right_pairs[i][0] == -1) { /* any right primer, check the left primer */ if ((l_start >= sa->ok_regions.left_pairs[i][0]) && (l_end <= sa->ok_regions.left_pairs[i][0] + sa->ok_regions.left_pairs[i][1] - 1)) { included = 1; break; } } else { /* check both primers */ if ((l_start >= sa->ok_regions.left_pairs[i][0]) && (l_end <= sa->ok_regions.left_pairs[i][0] + sa->ok_regions.left_pairs[i][1] - 1) && (r_start >= sa->ok_regions.right_pairs[i][0]) && (r_end <= sa->ok_regions.right_pairs[i][0] + sa->ok_regions.right_pairs[i][1] - 1)) { included = 1; break; } } } if (!included) { if (update_stats) { pair_expl->not_in_any_ok_region++; } if (!must_use) return PAIR_FAILED; } } /* ============================================================= */ /* Compute product Tm and related parameters; check constraints. */ if (ppair->right->start - ppair->left->start + 1 <= 0) { fprintf(stderr, "temporary"); } PR_ASSERT(ppair->right->start - ppair->left->start + 1 > 0) ppair->product_tm = long_seq_tm(sa->trimmed_seq, ppair->left->start, ppair->right->start - ppair->left->start + 1, /* TO DO -- skewed, it would be better to not use p_args elements here */ pa->p_args.salt_conc, pa->p_args.divalent_conc, pa->p_args.dntp_conc); PR_ASSERT(ppair->product_tm != OLIGOTM_ERROR); min_oligo_tm = ppair->left->temp > ppair->right->temp ? ppair->right->temp : ppair->left->temp; ppair->product_tm_oligo_tm_diff = ppair->product_tm - min_oligo_tm; ppair->t_opt_a = 0.3 * min_oligo_tm + 0.7 * ppair->product_tm - 14.9; if (pa->product_min_tm != PR_DEFAULT_PRODUCT_MIN_TM && ppair->product_tm < pa->product_min_tm) { if (update_stats) { pair_expl->low_tm++; } if (!must_use) return PAIR_FAILED; } if (pa->product_max_tm != PR_DEFAULT_PRODUCT_MAX_TM && ppair->product_tm > pa->product_max_tm) { if (update_stats) { pair_expl->high_tm++; } if (!must_use) return PAIR_FAILED; } ppair->diff_tm = fabs(retval->fwd.oligo[m].temp - retval->rev.oligo[n].temp); if (ppair->diff_tm > pa->max_diff_tm) { if (update_stats) { pair_expl->temp_diff++; } if (!must_use) return PAIR_FAILED; } /* End of product-temperature related computations. */ /* ============================================================= */ /* ============================================================= */ /* BEGIN "EXPENSIVE" computations on _individual_ primers in the pair. These have been postponed until this point in the interests of efficiency. */ /* ============================================================= */ /* Estimate secondary structure and primer-dimer of _individual_ primers if not already calculated. */ /* s1 is the forward oligo. */ _pr_substr(sa->trimmed_seq, retval->fwd.oligo[m].start, retval->fwd.oligo[m].length, s1); /* s2 is the reverse oligo. */ _pr_substr(sa->trimmed_seq, retval->rev.oligo[n].start - retval->rev.oligo[n].length + 1, retval->rev.oligo[n].length, s2); p3_reverse_complement(s1, s1_rev); p3_reverse_complement(s2, s2_rev); if (retval->fwd.oligo[m].self_any == ALIGN_SCORE_UNDEF && pa->thermodynamic_oligo_alignment==0) { /* We have not yet computed the 'self_any' paramter, which is an estimate of self primer-dimer and secondary structure propensity. */ oligo_compl(&retval->fwd.oligo[m], &pa->p_args, &retval->fwd.expl, dpal_arg_to_use, s1, s1_rev); if (!OK_OR_MUST_USE(&retval->fwd.oligo[m])) { pair_expl->considered--; if (!must_use) return PAIR_FAILED; } } /* Thermodynamic approach, fwd-primer */ if (retval->fwd.oligo[m].self_any == ALIGN_SCORE_UNDEF && pa->thermodynamic_oligo_alignment==1) { oligo_compl_thermod(&retval->fwd.oligo[m], &pa->p_args, &retval->fwd.expl, thal_arg_to_use, s1, s1); /* ! s1, s1_rev */ if (!OK_OR_MUST_USE(&retval->fwd.oligo[m])) { pair_expl->considered--; if (!must_use) return PAIR_FAILED; } } if (retval->fwd.oligo[m].hairpin_th == ALIGN_SCORE_UNDEF && pa->thermodynamic_oligo_alignment==1) { oligo_hairpin(&retval->fwd.oligo[m], &pa->p_args, &retval->fwd.expl, thal_arg_to_use, s1); if (!OK_OR_MUST_USE(&retval->fwd.oligo[m])) { pair_expl->considered--; if (!must_use) return PAIR_FAILED; } } /* End of thermodynamic approach */ if (retval->rev.oligo[n].self_any == ALIGN_SCORE_UNDEF && pa->thermodynamic_oligo_alignment==0) { oligo_compl(&retval->rev.oligo[n], &pa->p_args, &retval->rev.expl, dpal_arg_to_use, s2_rev, s2); if (!OK_OR_MUST_USE(&retval->rev.oligo[n])) { pair_expl->considered--; if (!must_use) return PAIR_FAILED; } } /* Thermodynamic approach */ if (retval->rev.oligo[n].self_any == ALIGN_SCORE_UNDEF && pa->thermodynamic_oligo_alignment==1) { oligo_compl_thermod(&retval->rev.oligo[n], &pa->p_args, &retval->rev.expl, thal_arg_to_use, s2_rev, s2_rev); /* s2_rev, s2 */ if (!OK_OR_MUST_USE(&retval->rev.oligo[n])) { pair_expl->considered--; if (!must_use) return PAIR_FAILED; } } if (retval->rev.oligo[n].hairpin_th == ALIGN_SCORE_UNDEF && pa->thermodynamic_oligo_alignment==1) { oligo_hairpin(&retval->rev.oligo[n], &pa->p_args, &retval->rev.expl, thal_arg_to_use, s2_rev); if (!OK_OR_MUST_USE(&retval->rev.oligo[n])) { pair_expl->considered--; if (!must_use) return PAIR_FAILED; } } /* End of thermodynamic approach */ /* End of secondary structure and primer-dimer of _individual_ primers. */ /* ============================================================= */ /* ============================================================= */ /* Mispriming of _individual_ primers to template and to repeat libraries. */ if (retval->fwd.oligo[m].repeat_sim.score == NULL) { /* We have not yet checked the oligo against the repeat library. */ oligo_repeat_library_mispriming(&retval->fwd.oligo[m], pa, sa, OT_LEFT, &retval->fwd.expl,dpal_arg_to_use); if (OK_OR_MUST_USE(&retval->fwd.oligo[m])) { oligo_template_mispriming(&retval->fwd.oligo[m], pa, sa, OT_LEFT, &retval->fwd.expl, dpal_arg_to_use->local_end, thal_args_for_template_mispriming); } if (!OK_OR_MUST_USE(&retval->fwd.oligo[m])) { pair_expl->considered--; if (!must_use) return PAIR_FAILED; } } if (retval->rev.oligo[n].repeat_sim.score == NULL) { oligo_repeat_library_mispriming(&retval->rev.oligo[n], pa, sa, OT_RIGHT, &retval->rev.expl, dpal_arg_to_use); if (OK_OR_MUST_USE(&retval->rev.oligo[n])) { oligo_template_mispriming(&retval->rev.oligo[n], pa, sa, OT_RIGHT, &retval->rev.expl, dpal_arg_to_use->local_end, thal_args_for_template_mispriming); } if (!OK_OR_MUST_USE(&retval->rev.oligo[n])) { pair_expl->considered--; if (!must_use) return PAIR_FAILED; } } /* End of mispriming of _individual_ primers to template and to repeat libraries. */ /* ============================================================= */ /* ============================================================= */ /* * Similarity between s1 and s2 is equivalent to complementarity between * s2's complement and s1. (Both s1 and s2 are taken from the same strand.) */ if(pa->thermodynamic_oligo_alignment==0) { ppair->compl_any = align(s1, s2, dpal_arg_to_use->local); if (ppair->compl_any > pa->pair_compl_any) { if (update_stats) { pair_expl->compl_any++; } if (!must_use) return PAIR_FAILED; } ppair->compl_end = align(s1, s2, dpal_arg_to_use->end); if (ppair->compl_end > pa->pair_compl_end) { if (update_stats) { pair_expl->compl_end++; } if (!must_use) return PAIR_FAILED; } } else { /* thermodynamical approach */ ppair->compl_any = align_thermod(s1, s2_rev, thal_arg_to_use->any); if (ppair->compl_any > pa->pair_compl_any_th) { if (update_stats) { pair_expl->compl_any++; } if (!must_use) return PAIR_FAILED; } ppair->compl_end = align_thermod(s1, s2_rev, thal_arg_to_use->end1); compl_end = align_thermod(s1, s2_rev, thal_arg_to_use->end2); /* Triinu Please check */ if (ppair->compl_end < compl_end) { ppair->compl_end = compl_end; } if (ppair->compl_end > pa->pair_compl_end_th) { if (update_stats) { pair_expl->compl_end++; } if (!must_use) return PAIR_FAILED; } } /* * It is conceivable (though unlikely) that * align(s2_rev, s1_rev, end_args) > align(s1,s2,end_args). */ if (pa->thermodynamic_oligo_alignment==0 && (compl_end = align(s2_rev, s1_rev, dpal_arg_to_use->end)) > ppair->compl_end) { if (compl_end > pa->p_args.max_self_end) { if (update_stats) { pair_expl->compl_end++; } if (!must_use) return PAIR_FAILED; } ppair->compl_end = compl_end; } if ((ppair->repeat_sim = pair_repeat_sim(ppair, pa)) > pa->pair_repeat_compl) { if (update_stats) { pair_expl->repeat_sim++; } if (!must_use) return PAIR_FAILED; } /* thermodynamic approach */ if (pa->thermodynamic_oligo_alignment==1 && ((compl_end = align_thermod(s2, s1_rev, thal_arg_to_use->end1)) > ppair->compl_end || (compl_end = align_thermod(s2, s1_rev, thal_arg_to_use->end2)) > ppair->compl_end)) { if (compl_end > pa->p_args.max_self_end_th) { if (update_stats) { pair_expl->compl_end++; } if (!must_use) return PAIR_FAILED; } ppair->compl_end = compl_end; } /* ============================================================= */ /* ============================================================= */ /* Calculate _pair_ mispriming, if necessary. */ if (pa->thermodynamic_template_alignment == 0) { if (!_pr_need_pair_template_mispriming(pa)) ppair->template_mispriming = ALIGN_SCORE_UNDEF; else { PR_ASSERT(ppair->left->template_mispriming != ALIGN_SCORE_UNDEF); PR_ASSERT(ppair->left->template_mispriming_r != ALIGN_SCORE_UNDEF); PR_ASSERT(ppair->right->template_mispriming != ALIGN_SCORE_UNDEF); PR_ASSERT(ppair->right->template_mispriming_r != ALIGN_SCORE_UNDEF); ppair->template_mispriming = ppair->left->template_mispriming + ppair->right->template_mispriming_r; if ((ppair->left->template_mispriming_r + ppair->right->template_mispriming) > ppair->template_mispriming) ppair->template_mispriming = ppair->left->template_mispriming_r + ppair->right->template_mispriming; if (pa->pair_max_template_mispriming >= 0.0 && ppair->template_mispriming > pa->pair_max_template_mispriming) { if (update_stats) { pair_expl->template_mispriming++; } if (!must_use) return PAIR_FAILED; } } } else { /* thermodynamic approach */ if (!_pr_need_pair_template_mispriming_thermod(pa)) ppair->template_mispriming = ALIGN_SCORE_UNDEF; else { PR_ASSERT(ppair->left->template_mispriming != ALIGN_SCORE_UNDEF); PR_ASSERT(ppair->left->template_mispriming_r != ALIGN_SCORE_UNDEF); PR_ASSERT(ppair->right->template_mispriming != ALIGN_SCORE_UNDEF); PR_ASSERT(ppair->right->template_mispriming_r != ALIGN_SCORE_UNDEF); ppair->template_mispriming = ppair->left->template_mispriming + ppair->right->template_mispriming_r; if ((ppair->left->template_mispriming_r + ppair->right->template_mispriming) > ppair->template_mispriming) ppair->template_mispriming = ppair->left->template_mispriming_r + ppair->right->template_mispriming; if (pa->pair_max_template_mispriming_th && ppair->template_mispriming > pa->pair_max_template_mispriming_th) { if (update_stats) { pair_expl->template_mispriming++; } if (!must_use) return PAIR_FAILED; } } } /* End of calculating _pair_ mispriming if necessary. */ /* ============================================================= */ return PAIR_OK; } /* characterize_pair */ /* */ void compute_position_penalty(const p3_global_settings *pa, const seq_args *sa, primer_rec *h, oligo_type o_type) { int three_prime_base; int inside_flag = 0; int target_begin, target_end; PR_ASSERT(OT_LEFT == o_type || OT_RIGHT == o_type); PR_ASSERT(1 == sa->tar2.count); target_begin = sa->tar2.pairs[0][0]; target_end = target_begin + sa->tar2.pairs[0][1] - 1; three_prime_base = OT_LEFT == o_type ? h->start + h->length - 1 : h->start - h->length + 1; bf_set_infinite_pos_penalty(h,1); h->position_penalty = 0.0; if (OT_LEFT == o_type) { if (three_prime_base <= target_end) { bf_set_infinite_pos_penalty(h,0); if (three_prime_base < target_begin) h->position_penalty = target_begin - three_prime_base - 1; else { h->position_penalty = three_prime_base - target_begin + 1; inside_flag = 1; } } } else { /* OT_RIGHT == o_type */ if (three_prime_base >= target_begin) { bf_set_infinite_pos_penalty(h,0); if (three_prime_base > target_end) { h->position_penalty = three_prime_base - target_end - 1; } else { h->position_penalty = target_end - three_prime_base + 1; inside_flag = 1; } } } if (!inside_flag) h->position_penalty *= pa->outside_penalty; else { h->position_penalty *= pa->inside_penalty; } } /* * Return 1 if 'pair' spans any target (in sa->tar); otherwise return 0; An * oligo pair spans a target, t, if the last base of the left primer is to * left of the last base of t and the first base of the right primer is to * the right of the first base of t. Of course the primers must * still be in a legal position with respect to each other. */ static int pair_spans_target(const primer_pair *pair, const seq_args *sa) { int i; int last_of_left = pair->left->start + pair->left->length - 1; int first_of_right = pair->right->start - pair->right->length + 1; int target_first, target_last; for (i = 0; i < sa->tar2.count; i++) { target_first = sa->tar2.pairs[i][0]; target_last = target_first + sa->tar2.pairs[i][1] - 1; if (last_of_left <= target_last && first_of_right >= target_first && last_of_left < first_of_right) return 1; } return 0; } /* * Return the value of the objective function value for given primer pair. * We must know that the pair is acceptable before calling obj_fn. */ static double obj_fn(const p3_global_settings *pa, primer_pair *h) { double sum; double lower_tm; sum = 0.0; lower_tm = h->right->temp; if(h->left->temp < h->right->temp) lower_tm = h->left->temp; if(pa->pr_pair_weights.primer_quality) /* HERE 1 */ sum += pa->pr_pair_weights.primer_quality * (h->left->quality + h->right->quality); if(pa->pr_pair_weights.io_quality && pa->pick_right_primer && pa->pick_left_primer && pa->pick_internal_oligo) sum += pa->pr_pair_weights.io_quality * h->intl->quality; if(pa->pr_pair_weights.diff_tm) sum += pa->pr_pair_weights.diff_tm * h->diff_tm; if (pa->thermodynamic_oligo_alignment==0) { if (pa->pr_pair_weights.compl_any) sum += pa->pr_pair_weights.compl_any * h->compl_any; if (pa->pr_pair_weights.compl_end) sum += pa->pr_pair_weights.compl_end * h->compl_end; } else if (pa->thermodynamic_oligo_alignment==1) { if (pa->pr_pair_weights.compl_any_th && ((lower_tm - pa->pr_pair_weights.temp_cutoff) <= h->compl_any)) sum += pa->pr_pair_weights.compl_any_th * (h->compl_any - (lower_tm - pa->pr_pair_weights.temp_cutoff - 1.0)); if (pa->pr_pair_weights.compl_any_th && ((lower_tm - pa->pr_pair_weights.temp_cutoff) > h->compl_any)) sum += pa->pr_pair_weights.compl_any_th * (1/(lower_tm - pa->pr_pair_weights.temp_cutoff + 1.0 - h->compl_any)); if (pa->pr_pair_weights.compl_end_th && ((lower_tm - pa->pr_pair_weights.temp_cutoff) <= h->compl_end)) sum += pa->pr_pair_weights.compl_end_th * (h->compl_end - (lower_tm - pa->pr_pair_weights.temp_cutoff - 1.0)); if (pa->pr_pair_weights.compl_end_th && ((lower_tm - pa->pr_pair_weights.temp_cutoff) > h->compl_end)) sum += pa->pr_pair_weights.compl_end_th * (1/(lower_tm - pa->pr_pair_weights.temp_cutoff + 1.0 - h->compl_end)); } else { PR_ASSERT(0); /* Programming error */ } if(pa->pr_pair_weights.product_tm_lt && h->product_tm < pa->product_opt_tm) sum += pa->pr_pair_weights.product_tm_lt * (pa->product_opt_tm - h->product_tm); if(pa->pr_pair_weights.product_tm_gt && h->product_tm > pa->product_opt_tm) sum += pa->pr_pair_weights.product_tm_gt * (h->product_tm - pa->product_opt_tm); if(pa->pr_pair_weights.product_size_lt && h->product_size < pa->product_opt_size) sum += pa->pr_pair_weights.product_size_lt * (pa->product_opt_size - h->product_size); if(pa->pr_pair_weights.product_size_gt && h->product_size > pa->product_opt_size) sum += pa->pr_pair_weights.product_size_gt * (h->product_size - pa->product_opt_size); if(pa->pr_pair_weights.repeat_sim) sum += pa->pr_pair_weights.repeat_sim * h->repeat_sim; if (pa->pr_pair_weights.template_mispriming && pa->thermodynamic_template_alignment==0) { PR_ASSERT(pa->pr_pair_weights.template_mispriming >= 0.0); PR_ASSERT(h->template_mispriming >= 0.0); sum += pa->pr_pair_weights.template_mispriming * h->template_mispriming; } if (pa->pr_pair_weights.template_mispriming_th && pa->thermodynamic_template_alignment==1) { PR_ASSERT(pa->pr_pair_weights.template_mispriming_th >= 0.0); PR_ASSERT(h->template_mispriming >= 0.0); if((lower_tm - pa->pr_pair_weights.temp_cutoff) <= h->template_mispriming) sum += pa->pr_pair_weights.template_mispriming_th * (h->template_mispriming - (lower_tm - pa->pr_pair_weights.temp_cutoff - 1.0)); if((lower_tm - pa->pr_pair_weights.temp_cutoff) > h->template_mispriming) sum += pa->pr_pair_weights.template_mispriming_th * (1/(lower_tm - pa->pr_pair_weights.temp_cutoff + 1.0 - h->template_mispriming)); } PR_ASSERT(sum >= 0.0); return sum; } static double align(const char *s1, const char *s2, const dpal_args *a) { dpal_results r; if(a->flag == DPAL_LOCAL || a->flag == DPAL_LOCAL_END) { if (strlen(s2) < 3) { /* For extremely short alignments we simply max out the score, because the dpal subroutines for these cannot handle this case. TO DO: this can probably be corrected in dpal. */ return strlen(s2); } } dpal((const unsigned char *) s1, (const unsigned char *) s2, a, &r); PR_ASSERT(r.score <= SHRT_MAX); if (r.score == DPAL_ERROR_SCORE) { /* There was an error. */ if (errno == ENOMEM) { longjmp(_jmp_buf, 1); } else { /* This branch is taken only if there is a programming error, in that s1 or s2 were NULL or contained an illegal character. We try to print some debugging information before aborting. */ fprintf(stderr, "%s", r.msg); /* Always false, causes an abort: */ PR_ASSERT(r.score != DPAL_ERROR_SCORE); } } return ((r.score < 0.0) ? 0.0 : r.score / PR_ALIGN_SCORE_PRECISION); } /* Helper function */ static int _set_string(char **loc, const char *new_string) { if (*loc) { free(*loc); } if (!(*loc = (char *) malloc(strlen(new_string) + 1))) return 1; /* ENOMEM */ strcpy(*loc, new_string); return 0; } static double align_thermod(const char *s1, const char *s2, const thal_args *a) { int thal_trace=0; thal_results r; thal((const unsigned char *) s1, (const unsigned char *) s2, a, &r); if (thal_trace) { fprintf(stdout, "thal, thal_args, type=%d maxLoop=%d mv=%f dv=%f " "dntp=%f dna_conc=%f, temp=%f, temponly=%d dimer=%d\n", a->type, a->maxLoop, a->mv, a->dv, a->dntp, a->dna_conc, a->temp, a->temponly, a->dimer); fprintf(stdout, "thal: s1=%s s2=%s temp=%f msg=%s end1=%d end2=%d\n", s1, s2, r.temp, r.msg, r.align_end_1, r.align_end_2); } PR_ASSERT(r.temp <= DBL_MAX); if (r.temp == THAL_ERROR_SCORE) { /* There was an error. */ if (errno == ENOMEM) { longjmp(_jmp_buf, 1); } else { /* This branch is taken if there is an error other than ENOMEM, in which case we treat it as a per sequence error. */ /* Save the error string */ if (!_set_string(&thermodynamic_alignment_length_error_msg, r.msg)) { thermodynamic_alignment_length_error = 1; } longjmp(_jmp_buf, 1); } } return ((r.temp < 0.0) ? 0.0 : (double) (r.temp)); } /* Return the sequence of oligo in a ****static***** buffer. The sequence returned is changed at the next call to pr_oligo_sequence. */ char * pr_oligo_sequence(const seq_args *sa, const primer_rec *oligo) { static char s[MAX_PRIMER_LENGTH+1]; int seq_len; PR_ASSERT(NULL != sa); PR_ASSERT(NULL != oligo); seq_len = strlen(sa->sequence); PR_ASSERT(oligo->start + sa->incl_s >= 0); PR_ASSERT(oligo->start + sa->incl_s + oligo->length <= seq_len); _pr_substr(sa->sequence, sa->incl_s + oligo->start, oligo->length, s); return &s[0]; } char * pr_oligo_rev_c_sequence(const seq_args *sa, const primer_rec *o) { static char s[MAX_PRIMER_LENGTH+1], s1[MAX_PRIMER_LENGTH+1]; int seq_len, start; PR_ASSERT(NULL != sa); PR_ASSERT(NULL != o); seq_len = strlen(sa->sequence); start = sa->incl_s + o->start - o->length + 1; PR_ASSERT(start >= 0); PR_ASSERT(start + o->length <= seq_len); _pr_substr(sa->sequence, start, o->length, s); p3_reverse_complement(s,s1); return &s1[0]; } /* Calculate self complementarity (both h->self_any and h->self_end) which we use as an approximation for both secondary structure and self primer-dimer. */ static void oligo_compl(primer_rec *h, const args_for_one_oligo_or_primer *po_args, oligo_stats *ostats, const dpal_arg_holder *dpal_arg_to_use, const char *oligo_seq, const char *revc_oligo_seq) { PR_ASSERT(h != NULL); h->self_any = align(oligo_seq, revc_oligo_seq, dpal_arg_to_use->local); if (h->self_any > po_args->max_self_any) { op_set_high_self_any(h); ostats->compl_any++; ostats->ok--; if (!h->must_use) return; } h->self_end = align(oligo_seq, revc_oligo_seq, dpal_arg_to_use->end); if (h->self_end > po_args->max_self_end) { op_set_high_self_end(h); ostats->compl_end++; ostats->ok--; if (!h->must_use) return; } } static void oligo_compl_thermod(primer_rec *h, const args_for_one_oligo_or_primer *po_args, oligo_stats *ostats, const thal_arg_holder *thal_arg_to_use, const char *oligo_seq, const char *revc_oligo_seq) { PR_ASSERT(h != NULL); h->self_any = align_thermod(oligo_seq, revc_oligo_seq, thal_arg_to_use->any); if(h->self_any > po_args->max_self_any_th) { op_set_high_self_any(h); ostats->compl_any++; ostats->ok--; if (!h->must_use) return; } h->self_end = align_thermod(oligo_seq, revc_oligo_seq, thal_arg_to_use->end1); if(h->self_end > po_args->max_self_end_th){ op_set_high_self_end(h); ostats->compl_end++; ostats->ok--; if (!h->must_use) return; } } static void oligo_hairpin(primer_rec *h, const args_for_one_oligo_or_primer *po_args, oligo_stats *ostats, const thal_arg_holder *thal_arg_to_use, const char *oligo_seq) { PR_ASSERT(h != NULL); h->hairpin_th = align_thermod(oligo_seq, oligo_seq, thal_arg_to_use->hairpin_th); if(h->hairpin_th > po_args->max_hairpin_th) { op_set_high_hairpin_th(h); ostats->hairpin_th++; ostats->ok--; return; } } static void primer_mispriming_to_template(primer_rec *h, const p3_global_settings *pa, const seq_args *sa, oligo_type l, oligo_stats *ostats, int first, int last, /* The oligo sequence: */ const char *s, /* s reverse complemented: */ const char *s_r, const dpal_args *align_args ) { const char *oseq; char *target, *target_r; int tmp, seqlen; int debug = 0; int first_untrimmed, last_untrimmed; /* Indexes of first and last bases of the oligo in sa->seq, that is, WITHIN THE TOTAL SEQUENCE INPUT. */ /* first, last are indexes of first and last bases of the oligo in sa->trimmed_seq, that is, WITHIN THE INCLUDED REGION. */ char tmp_char; double tmp_score; seqlen = strlen(sa->upcased_seq); first_untrimmed = sa->incl_s + first; last_untrimmed = sa->incl_s + last; if (l == OT_LEFT) { oseq = &s[0]; target = &sa->upcased_seq[0]; target_r = &sa->upcased_seq_r[0]; } else { /* l == OT_RIGHT */ if (debug) fprintf(stderr, "first_untrimmed = %d, last_untrimmed = %d\n", first_untrimmed, last_untrimmed); oseq = &s_r[0]; target = &sa->upcased_seq_r[0]; target_r = &sa->upcased_seq[0]; /* We need to adjust first_untrimmed and last_untrimmed so that they are correct in the reverse-complemented sequence. */ tmp = (seqlen - last_untrimmed) - 1; last_untrimmed = (seqlen - first_untrimmed) - 1; first_untrimmed = tmp; } /* 1. Align to the template 5' of the oligo. */ tmp_char = target[first_untrimmed]; target[first_untrimmed] = '\0'; tmp_score = align(oseq, target, align_args); if (debug) { if (l == OT_LEFT) fprintf(stderr, "\n************ OLIGO = LEFT\n"); else fprintf(stderr, "\n************ OLIGO = RIGHT\n"); fprintf(stderr, "first_untrimmed = %d, last_untrimmed = %d,first = %d, last = %d\n", first_untrimmed, last_untrimmed, first, last); fprintf(stderr, "5' of oligo: Score %f aligning %s against %s\n\n", tmp_score, oseq, target); } target[first_untrimmed] = tmp_char; /* 2. Align to the template 3' of the oligo. */ h->template_mispriming = align(oseq, &target[0] + last_untrimmed + 1, align_args); if (debug) fprintf(stderr, "3' of oligo Score %f aligning %s against %s\n\n", h->template_mispriming, oseq, &target[0] + last_untrimmed + 1); /* 3. Take the max of 1. and 2. */ if (tmp_score > h->template_mispriming) h->template_mispriming = tmp_score; /* 4. Align to the reverse strand of the template. */ h->template_mispriming_r = align(oseq, target_r, align_args); if (debug) fprintf(stderr, "other strand Score %f aligning %s against %s\n\n", h->template_mispriming_r, oseq, target_r); if (pa->p_args.max_template_mispriming >= 0) { if (oligo_max_template_mispriming(h) > pa->p_args.max_template_mispriming) { op_set_high_similarity_to_multiple_template_sites(h); if (OT_LEFT == l || OT_RIGHT == l ) { ostats->template_mispriming++; ostats->ok--; } else PR_ASSERT(0); /* Should not get here. */ } else { /* This oligo is ok, mark it so we do not do this check again. */ h->template_mispriming_ok = 1; } } } static void primer_mispriming_to_template_thermod(primer_rec *h, const p3_global_settings *pa, const seq_args *sa, oligo_type l, oligo_stats *ostats, int first, int last, /* The oligo sequence: */ const char *s, /* s reverse complemented: */ const char *s_r, const thal_args *align_args) { const char *oseq; char *target, *target_r; int tmp, seqlen; int debug = 0; int first_untrimmed, last_untrimmed; /* Indexes of first and last bases of the oligo in sa->seq, * that is, WITHIN THE TOTAL SEQUENCE INPUT. */ /* first, last are indexes of first and last bases of the oligo in * sa->trimmed_seq, that is, WITHIN THE INCLUDED REGION. */ char tmp_char; double tmp_score; seqlen = strlen(sa->upcased_seq); first_untrimmed = sa->incl_s + first; last_untrimmed = sa->incl_s + last; if (l == OT_RIGHT) { oseq = &s_r[0]; target = &sa->upcased_seq[0]; target_r = &sa->upcased_seq_r[0]; } else { /* l == OT_RIGHT */ if (debug) fprintf(stdout, "first_untrimmed = %d, last_untrimmed = %d\n", first_untrimmed, last_untrimmed); oseq = &s[0]; target = &sa->upcased_seq_r[0]; target_r = &sa->upcased_seq[0]; /* We need to adjust first_untrimmed and last_untrimmed so that * they are correct in the reverse-complemented * sequence. * */ tmp = (seqlen - last_untrimmed) - 1; last_untrimmed = (seqlen - first_untrimmed) - 1; first_untrimmed = tmp; } /* 1. Align to the template 5' of the oligo. */ /* tmp_char = target_r[first_untrimmed]; Corrected 2012-05-30 */ tmp_char = target[first_untrimmed]; target[first_untrimmed] = '\0'; tmp_score = align_thermod(oseq, target, align_args); if (debug) { if (l == OT_LEFT) fprintf(stdout, "\n************ OLIGO = LEFT\n"); else fprintf(stdout, "\n************ OLIGO = RIGHT\n"); fprintf(stdout, "first_untrimmed = %d, last_untrimmed = %d, first = %d, last = %d\n", first_untrimmed, last_untrimmed, first, last); fprintf(stdout, "5' of oligo: Score %f aligning %s against %s\n\n", tmp_score, oseq, target); } target[first_untrimmed] = tmp_char; /* 2. Align to the template 3' of the oligo. */ h->template_mispriming = align_thermod(oseq, &target[0] + last_untrimmed + 1, align_args); if (debug) fprintf(stdout, "3' of oligo Score %f aligning %s against %s\n\n", h->template_mispriming, oseq, &target[0] + last_untrimmed + 1); /* 3. Take the max of 1. and 2. */ if (tmp_score > h->template_mispriming) h->template_mispriming = tmp_score; /* 4. Align to the reverse strand of the template. */ h->template_mispriming_r = align_thermod(oseq, target_r, align_args); if (debug) fprintf(stdout, "In primer_mispriming_to_template_thermod\n" " other strand Score %f aligning %s against %s\n\n", h->template_mispriming_r, oseq, target_r); if (pa->p_args.max_template_mispriming_th >= 0) { if (oligo_max_template_mispriming_thermod(h) > pa->p_args.max_template_mispriming_th) { op_set_high_similarity_to_multiple_template_sites(h); if (OT_LEFT == l || OT_RIGHT == l ) { ostats->template_mispriming++; ostats->ok--; } else PR_ASSERT(0); /* Should not get here. */ } else { /* This oligo is ok, mark it so we do not do this check again. */ h->template_mispriming_ok = 1; } } } static void oligo_compute_sequence_and_reverse(primer_rec *h, const seq_args *sa, oligo_type l, int *first, int *last, char *s, char *s_r) { *first = (OT_LEFT == l || OT_INTL == l) ? h->start : h->start - h->length + 1; *last = (OT_LEFT == l || OT_INTL == l) ? h->start + h->length - 1 : h->start; _pr_substr(sa->trimmed_seq, *first, h->length, s); p3_reverse_complement(s, s_r); } /* Possible improvement -- pass in the oligo sequences */ static void oligo_repeat_library_mispriming(primer_rec *h, const p3_global_settings *pa, const seq_args *sa, oligo_type l, oligo_stats *ostats, const dpal_arg_holder *dpal_arg_to_use) { char s[MAX_PRIMER_LENGTH+1], /* Will contain the oligo sequence. */ s_r[MAX_PRIMER_LENGTH+1]; /* Will contain s reverse complemented. */ double w; const seq_lib *lib; int i; int first, last; /* Indexes of first and last bases of the oligo in sa->trimmed_seq, that is, WITHIN THE INCLUDED REGION. */ int min, max; short max_lib_compl; /* First, check the oligo against the repeat library. */ if (OT_INTL == l) { lib = pa->o_args.repeat_lib; max_lib_compl = (short) pa->o_args.max_repeat_compl; } else { lib = pa->p_args.repeat_lib; max_lib_compl = (short) pa->p_args.max_repeat_compl; } oligo_compute_sequence_and_reverse(h, sa, l, &first, &last, s, s_r); /* * Calculate maximum similarity to sequences from user defined repeat * library. Compare it with maximum allowed repeat similarity. */ if (seq_lib_num_seq(lib) > 0) { /* Library exists and is non-empty. */ h->repeat_sim.score = (double *) pr_safe_malloc(lib->seq_num * sizeof(double)); h->repeat_sim.max = h->repeat_sim.min = 0; max = min = 0; h->repeat_sim.name = lib->names[0]; for (i = 0; i < lib->seq_num; i++) { if (OT_LEFT == l) w = lib->weight[i] * align(s, lib->seqs[i], (pa->lib_ambiguity_codes_consensus ? dpal_arg_to_use->local_end_ambig : dpal_arg_to_use->local_end)); else if (OT_INTL == l) w = lib->weight[i] * align(s, lib->seqs[i], (pa->lib_ambiguity_codes_consensus ? dpal_arg_to_use->local_ambig : dpal_arg_to_use->local)); else w = lib->weight[i] * align(s_r, lib->rev_compl_seqs[i], (pa->lib_ambiguity_codes_consensus ? dpal_arg_to_use->local_end_ambig : dpal_arg_to_use->local)); if (w > SHRT_MAX || w < SHRT_MIN) { abort(); /* TO DO, propagate error */ /* This check is necessary for the next 9 lines */ } h->repeat_sim.score[i] = w; if(w > max){ max = (int) w; h->repeat_sim.max = i; h->repeat_sim.name = lib->names[i]; } if(w < min){ min = (int) w; h->repeat_sim.min = i; } if (w > max_lib_compl) { op_set_high_similarity_to_non_template_seq(h); ostats->repeat_score++; ostats->ok--; if (!h->must_use) return; } /* if w > max_lib_compl */ } /* for */ } /* if library exists and is non-empty */ /* End of checking against the repeat library */ } /* This function carries out either the old "dpal" alignment or the thermodynamic alignment, depending on the value of pa->thermodynamic_template_alignment. */ static void oligo_template_mispriming(primer_rec *h, const p3_global_settings *pa, const seq_args *sa, oligo_type l, oligo_stats *ostats, const dpal_args *d_align_args, const thal_args *t_align_args) { char s[MAX_PRIMER_LENGTH+1], /* Will contain the oligo sequence. */ s_r[MAX_PRIMER_LENGTH+1]; /* Will contain s reverse complemented. */ int first, last; /* Indexes of first and last bases of the oligo in sa->trimmed_seq, that is, WITHIN THE INCLUDED REGION. */ /* Check if we already did this and the oligo was ok. */ if (h->template_mispriming_ok) { return; } oligo_compute_sequence_and_reverse(h, sa, l, &first, &last, s, s_r); /* Calculate maximum similarity to ectopic sites in the template. */ if (l == OT_RIGHT || l == OT_LEFT) { if (pa->thermodynamic_template_alignment == 0 && _pr_need_template_mispriming(pa)) primer_mispriming_to_template(h, pa, sa, l, ostats, first, last, s, s_r, d_align_args); if (pa->thermodynamic_template_alignment == 1 && _pr_need_template_mispriming_thermod(pa)) primer_mispriming_to_template_thermod(h, pa, sa, l, ostats, first, last, s, s_r, t_align_args); } } static int pair_repeat_sim(primer_pair *h, const p3_global_settings *pa) { int i, n, max, w; primer_rec *fw, *rev; fw = h->left; rev = h->right; max = 0; n = seq_lib_num_seq(pa->p_args.repeat_lib); if(n == 0) return 0; h->rep_name = pa->p_args.repeat_lib->names[0] ; for (i = 0; i < n; i++) { if ((w = (int) (fw->repeat_sim.score[i] + rev->repeat_sim.score[i])) > max) { max = w; h->rep_name = pa->p_args.repeat_lib->names[i] ; } } return max; } static void set_retval_both_stop_codons(const seq_args *sa, p3retval *retval) { /* The position of the intial base of the rightmost stop codon that * is to the left of sa->start_codon_pos; valid only if * sa->start_codon_pos is "not null". We will not want to include * a stop codon to the right of the the start codon in the * amplicon. */ retval->upstream_stop_codon = find_stop_codon(sa->trimmed_seq, sa->start_codon_pos, -1); retval->upstream_stop_codon += sa->incl_s; retval->stop_codon_pos = find_stop_codon(sa->trimmed_seq, sa->start_codon_pos, 1); retval->stop_codon_pos += sa->incl_s; } /* * 's' is the sequence in which to find the stop codon. * 'start' is the position of a start codon. * * There are two modes depending on 'direction'. * * If direction is 1: * * Return the index of the first stop codon to the right of * 'start' in 's' (in same frame). * If there is no such stop codon return -1. * * If direction is -1: * * If 'start' is negative then return -1. * Otherwise return the index the first stop codon to left of 'start'. * If there is no such stop codon return -1. * * Note: we don't insist that the start codon be ATG, since in some * cases the caller will not have the full sequence in 's', nor even * know the postion of the start codon relative to s. */ static int find_stop_codon(const char* s, int start, int direction) { const char *p, *q; int increment = 3 * direction; int len = strlen(s); PR_ASSERT(s != NULL); PR_ASSERT(direction == 1 || direction == -1); PR_ASSERT(len >= 3); PR_ASSERT(start <= (len - 3)); if (start < 0) { if (direction == 1) while (start < 0) start += increment; else return -1; } for (p = &s[start]; p >= &s[0] && *p && *(p + 1) && *(p + 2); p += increment) { if ('T' != *p && 't' != *p) continue; q = p + 1; if ('A' == *q || 'a' == *q) { q++; if ('G' == *q || 'g' == *q || 'A' == *q || 'a' == *q) return p - &s[0]; } else if ('G' == *q || 'g' == *q) { q++; if ('A' == *q || 'a' == *q) return p - &s[0]; } } return -1; } int strcmp_nocase(const char *s1, const char *s2) { static char M[UCHAR_MAX]; static int f = 0; int i; const char *p, *q; if(f != 1){ for(i = 0; i < UCHAR_MAX; i++) M[i] = i; i = 'a'; M[i] = 'A'; i = 'b'; M[i] = 'B'; i = 'c'; M[i] = 'C'; i = 'A'; M[i] = 'a'; i = 'B'; M[i] = 'b'; i = 'C'; M[i] = 'c'; i = 'd'; M[i] = 'D'; i = 'e'; M[i] = 'E'; i = 'f'; M[i] = 'F'; i = 'D'; M[i] = 'd'; i = 'E'; M[i] = 'e'; i = 'F'; M[i] = 'f'; i = 'g'; M[i] = 'G'; i = 'h'; M[i] = 'H'; i = 'i'; M[i] = 'I'; i = 'G'; M[i] = 'g'; i = 'H'; M[i] = 'h'; i = 'I'; M[i] = 'i'; i = 'k'; M[i] = 'K'; i = 'l'; M[i] = 'L'; i = 'm'; M[i] = 'M'; i = 'K'; M[i] = 'k'; i = 'L'; M[i] = 'l'; i = 'M'; M[i] = 'm'; i = 'n'; M[i] = 'N'; i = 'o'; M[i] = 'O'; i = 'p'; M[i] = 'P'; i = 'N'; M[i] = 'n'; i = 'O'; M[i] = 'o'; i = 'P'; M[i] = 'p'; i = 'q'; M[i] = 'Q'; i = 'r'; M[i] = 'R'; i = 's'; M[i] = 'S'; i = 'Q'; M[i] = 'q'; i = 'R'; M[i] = 'r'; i = 'S'; M[i] = 's'; i = 't'; M[i] = 'T'; i = 'u'; M[i] = 'U'; i = 'v'; M[i] = 'V'; i = 'T'; M[i] = 't'; i = 'U'; M[i] = 'u'; i = 'V'; M[i] = 'v'; i = 'w'; M[i] = 'W'; i = 'x'; M[i] = 'X'; i = 'y'; M[i] = 'Y'; i = 'W'; M[i] = 'w'; i = 'X'; M[i] = 'x'; i = 'Y'; M[i] = 'y'; i = 'z'; M[i] = 'Z'; i = 'Z'; M[i] = 'z'; i = 'j'; M[i] = 'J'; i = 'J'; M[i] = 'j'; f = 1; } if(s1 == NULL || s2 == NULL) return 1; if(strlen(s1) != strlen(s2)) return 1; p = s1; q = s2; while(*p != '\0' && *p != '\n' && *q != '\0' && *q != '\n'){ i = *p; if(*p == *q || M[i] == *q ) {p++; q++; continue;} return 1; } return 0; } static void free_repeat_sim_score(p3retval *state) { int i; for (i = 0; i < state->fwd.num_elem; i++) { if (state->fwd.oligo[i].repeat_sim.score != NULL) { free(state->fwd.oligo[i].repeat_sim.score); state->fwd.oligo[i].repeat_sim.score = NULL; } } for (i = 0; i < state->rev.num_elem; i++) { if (state->rev.oligo[i].repeat_sim.score != NULL) { free(state->rev.oligo[i].repeat_sim.score); state->rev.oligo[i].repeat_sim.score = NULL; } } for (i = 0; i < state->intl.num_elem; i++) { if (state->intl.oligo[i].repeat_sim.score != NULL) { free(state->intl.oligo[i].repeat_sim.score); state->intl.oligo[i].repeat_sim.score = NULL; } } } static void free_primer_repeat_sim_score(primer_rec *h) { if (h->repeat_sim.score != NULL) { free(h->repeat_sim.score); h->repeat_sim.score = NULL; } } /* Edited by T. Koressaar for lowercase masking. This function checks if the 3' end of the primer has been masked by lowercase letter. Function created/Added by Eric Reppo, July 9, 2002 */ static int is_lowercase_masked(int position, const char *sequence, primer_rec *h, oligo_stats *global_oligo_stats) { const char* p = &sequence[position]; if ('a' == *p || 'c' == *p ||'g' == *p || 't' == *p) { op_set_overlaps_masked_sequence(h); global_oligo_stats->gmasked++; return 1; } return 0; } /* Edited by A. Untergasser */ static int primer_must_match(const p3_global_settings *pa, primer_rec *h, oligo_stats *global_oligo_stats, /* This is 5'->3' on the template sequence: */ const char *input_oligo_seq, char *match_three_prime, char *match_five_prime) { const char *seq; char *test; int length = h->length - 5; if (match_five_prime != NULL) { seq = input_oligo_seq; test = match_five_prime; for (int i = 0; i < 5; i++) { if (!compare_nucleotides(*seq, *test)) { global_oligo_stats->must_match_fail++; return 1; } seq++; test++; } } if (match_three_prime != NULL) { seq = input_oligo_seq; test = match_three_prime; seq = seq + length; for (int i = 0; i < 5; i++) { if (!compare_nucleotides(*seq, *test)) { global_oligo_stats->must_match_fail++; return 1; } seq++; test++; } } return 0; } /* For a [NACTG] is allowed, for b [NACTGRYWSMKBHDV].*/ static int compare_nucleotides(const char a, const char b) { char x = a; char y = b; /* Convert to uppercase */ if(a >= 'a' && a <= 'z'){ x = ('A' + a - 'a'); } if(b >= 'b' && b <= 'z'){ y = ('A' + b - 'a'); } if ( x == y ) { return 1; } if (( x == 'N') or (y == 'N')) { return 1; } if (x == 'A') { if ((y == 'R') or (y == 'W') or (y == 'M') or (y == 'H') or (y == 'D') or (y == 'V')){ return 1; } } if (x == 'G') { if ((y == 'R') or (y == 'S') or (y == 'K') or (y == 'B') or (y == 'D') or (y == 'V')){ return 1; } } if (x == 'C') { if ((y == 'Y') or (y == 'S') or (y == 'M') or (y == 'B') or (y == 'H') or (y == 'V')){ return 1; } } if (x == 'T') { if ((y == 'Y') or (y == 'W') or (y == 'K') or (y == 'B') or (y == 'H') or (y == 'D')){ return 1; } } return 0; } static int test_must_match_parameters(char *test) { int i = 0; char x; while (*test != '\0') { /* First to UPPER letters */ if ((*test >= 'a') && (*test <= 'z')){ x = ('A' + (*test) - 'a'); } else { x = *test; } /* Second check that it is in Range A-Z */ if ((x < 'A') || (x > 'Z')){ return 1; } /* Check it is NACTGRYWSMKBHDV */ if ((x == 'N') || (x == 'A') || (x == 'C') || (x == 'T') || (x == 'G') || (x == 'R') || (x == 'Y') || (x == 'W') || (x == 'S') || (x == 'M') || (x == 'K') || (x == 'B') || (x == 'H') || (x == 'D') || (x == 'V')){ test++; i++; } else { return 1; } } /* Check it is 5 letters */ if (i != 5) { return 1; } return 0; } /* Put substring of seq starting at n with length m into s. */ void _pr_substr(const char *seq, int n, int m, char *s) { int i; for(i=n;i= seq) { switch (*p) { case 'A': *q='T'; break; case 'C': *q='G'; break; case 'G': *q='C'; break; case 'T': *q='A'; break; case 'U': *q='A'; break; case 'B': *q='V'; break; case 'D': *q='H'; break; case 'H': *q='D'; break; case 'V': *q='B'; break; case 'R': *q='Y'; break; case 'Y': *q='R'; break; case 'K': *q='M'; break; case 'M': *q='K'; break; case 'S': *q='S'; break; case 'W': *q='W'; break; case 'N': *q='N'; break; case 'a': *q='t'; break; case 'c': *q='g'; break; case 'g': *q='c'; break; case 't': *q='a'; break; case 'u': *q='a'; break; case 'b': *q='v'; break; case 'd': *q='h'; break; case 'h': *q='d'; break; case 'v': *q='b'; break; case 'r': *q='y'; break; case 'y': *q='r'; break; case 'k': *q='m'; break; case 'm': *q='k'; break; case 's': *q='s'; break; case 'w': *q='w'; break; case 'n': *q='n'; break; } p--; q++; } *q = '\0'; } int _pr_need_template_mispriming(const p3_global_settings *pa) { return pa->p_args.max_template_mispriming >= 0 || pa->p_args.weights.template_mispriming > 0.0 || _pr_need_pair_template_mispriming(pa); } int _pr_need_template_mispriming_thermod(const p3_global_settings *pa) { return pa->p_args.max_template_mispriming_th >= 0 || pa->p_args.weights.template_mispriming_th > 0.0 || _pr_need_pair_template_mispriming_thermod(pa); } int _pr_need_pair_template_mispriming(const p3_global_settings *pa) { return pa->pair_max_template_mispriming >= 0 || pa->pr_pair_weights.template_mispriming > 0.0; } int _pr_need_pair_template_mispriming_thermod(const p3_global_settings *pa) { return pa->pair_max_template_mispriming_th >= 0 || pa->pr_pair_weights.template_mispriming_th > 0.0; } /* Upcase a DNA string, s, in place. If amibiguity_code_ok is false, then the characters acgtnACGTN are 'recognized' and upcased. If ambiguity_code_ok is true, then the IUB/IUPAC ambiguity codes are also 'recognized' and upcased. Change any unrecognized letters in *s to 'N" Return the first unrecognized letter, if any, that is seen. Otherwise return '\0'. */ static char dna_to_upper(char * s, int ambiguity_code_ok) { char *p = s; int unrecognized_base = '\0'; while (*p) { switch (*p) { case 'a': case 'A': *p='A'; break; case 'c': case 'C': *p='C'; break; case 'g': case 'G': *p='G'; break; case 't': case 'T': *p='T'; break; case 'n': case 'N': *p='N'; break; default: if (ambiguity_code_ok) { switch (*p) { case 'r': case 'R': *p = 'R'; break; case 'y': case 'Y': *p = 'Y'; break; case 'm': case 'M': *p = 'M'; break; case 'w': case 'W': *p = 'W'; break; case 's': case 'S': *p = 'S'; break; case 'k': case 'K': *p = 'K'; break; case 'd': case 'D': *p = 'D'; break; case 'h': case 'H': *p = 'H'; break; case 'v': case 'V': *p = 'V'; break; case 'b': case 'B': *p = 'B'; break; } } else { if (!unrecognized_base) unrecognized_base = *p; *p = 'N'; } break; } p++; } return unrecognized_base; } static char * strstr_nocase(char *s1, char *s2) { int n1, n2; char *p, q, *tmp; if(s1 == NULL || s2 == NULL) return NULL; n1 = strlen(s1); n2 = strlen(s2); if(n1 < n2) return NULL; tmp = (char *) pr_safe_malloc(n1 + 1); strcpy(tmp, s1); q = *tmp; p = tmp; while(q != '\0' && q != '\n'){ q = *(p + n2); *(p + n2) = '\0'; if(strcmp_nocase(p, s2)){ *(p + n2) = q; p++; continue; } else {free(tmp); return p;} } free(tmp); return NULL; } #define CHECK if (r > bsize || r < 0) return "Internal error, not enough space for \"explain\" string"; bufp += r; bsize -= r #define SP_AND_CHECK(FMT, VAL) { r = sprintf(bufp, FMT, VAL); CHECK; } #define IF_SP_AND_CHECK(FMT, VAL) { if (VAL) { SP_AND_CHECK(FMT, VAL) } } const char * p3_pair_explain_string(const pair_stats *pair_expl) { /* WARNING WARNING WARNING Static, fixed-size buffer. If you add more calls to SP_AND_CHECK or IF_SP_AND_CHECK, you need to make sure the buffer cannot overflow. */ static char buf[10000]; char *bufp = buf; size_t bsize = 10000; size_t r; SP_AND_CHECK("considered %d", pair_expl->considered) IF_SP_AND_CHECK(", no target %d", pair_expl->target) IF_SP_AND_CHECK(", unacceptable product size %d", pair_expl->product) IF_SP_AND_CHECK(", low product Tm %d", pair_expl->low_tm) IF_SP_AND_CHECK(", high product Tm %d", pair_expl->high_tm) IF_SP_AND_CHECK(", tm diff too large %d",pair_expl->temp_diff) IF_SP_AND_CHECK(", high any compl %d", pair_expl->compl_any) IF_SP_AND_CHECK(", high end compl %d", pair_expl->compl_end) IF_SP_AND_CHECK(", no internal oligo %d", pair_expl->internal) IF_SP_AND_CHECK(", high mispriming library similarity %d", pair_expl->repeat_sim) IF_SP_AND_CHECK(", no overlap of required point %d", pair_expl->does_not_overlap_a_required_point) IF_SP_AND_CHECK(", primer in pair overlaps a primer in a better pair %d", pair_expl->overlaps_oligo_in_better_pair) IF_SP_AND_CHECK(", high template mispriming score %d", pair_expl->template_mispriming) IF_SP_AND_CHECK(", not in any ok region %d", pair_expl->not_in_any_ok_region); IF_SP_AND_CHECK(", left primer to right of right primer %d", pair_expl->reversed); SP_AND_CHECK(", ok %d", pair_expl->ok) return buf; } const char * p3_oligo_explain_string(const oligo_stats *stat) { /* WARNING WARNING WARNING Static, fixed-size buffer. If you add more calls to SP_AND_CHECK or IF_SP_AND_CHECK, you need to make sure the buffer cannot overflow. */ static char buf[10000]; char *bufp = buf; size_t bsize = 10000; size_t r; SP_AND_CHECK("considered %d", stat->considered) IF_SP_AND_CHECK(", would not amplify any of the ORF %d", stat->no_orf) IF_SP_AND_CHECK(", too many Ns %d", stat->ns) IF_SP_AND_CHECK(", overlap target %d", stat->target) IF_SP_AND_CHECK(", overlap excluded region %d", stat->excluded) IF_SP_AND_CHECK(", GC content failed %d", stat->gc) IF_SP_AND_CHECK(", GC clamp failed %d", stat->gc_clamp) IF_SP_AND_CHECK(", low tm %d", stat->temp_min) IF_SP_AND_CHECK(", high tm %d", stat->temp_max) IF_SP_AND_CHECK(", high any compl %d", stat->compl_any) IF_SP_AND_CHECK(", high end compl %d", stat->compl_end) IF_SP_AND_CHECK(", high hairpin stability %d", stat->hairpin_th) IF_SP_AND_CHECK(", high repeat similarity %d", stat->repeat_score) IF_SP_AND_CHECK(", long poly-x seq %d", stat->poly_x) IF_SP_AND_CHECK(", low sequence quality %d", stat->seq_quality) IF_SP_AND_CHECK(", high 3' stability %d", stat->stability) IF_SP_AND_CHECK(", high template mispriming score %d", stat->template_mispriming) IF_SP_AND_CHECK(", lowercase masking of 3' end %d",stat->gmasked) IF_SP_AND_CHECK(", failed must_match requirements %d",stat->must_match_fail) IF_SP_AND_CHECK(", not in any ok left region %d", stat->not_in_any_left_ok_region) IF_SP_AND_CHECK(", not in any ok right region %d", stat->not_in_any_right_ok_region) SP_AND_CHECK(", ok %d", stat->ok) return buf; } #undef CHECK #undef SP_AND_CHECK #undef IF_SP_AND_CHEC const char * p3_get_oligo_array_explain_string(const oligo_array *oligo_array) { return p3_oligo_explain_string(&oligo_array->expl); } const char * p3_get_pair_array_explain_string(const pair_array_t *pair_array) { return p3_pair_explain_string(&pair_array->expl); } const char * libprimer3_release(void) { return "libprimer3 release 2.3.6"; } const char * primer3_copyright(void) { return primer3_copyright_char_star; } /* ============================================================ */ /* BEGIN Internal and external functions for pr_append_str */ /* ============================================================ */ void init_pr_append_str(pr_append_str *s) { s->data = NULL; s->storage_size = 0; } const char * pr_append_str_chars(const pr_append_str *x) { return x->data; } pr_append_str * create_pr_append_str() { /* We cannot use pr_safe_malloc here because this function will be called outside of the setjmp(....) */ pr_append_str *ret; ret = (pr_append_str *) malloc(sizeof(pr_append_str)); if (NULL == ret) return NULL; init_pr_append_str(ret); return ret; } void destroy_pr_append_str_data(pr_append_str *str) { if (NULL == str) return; free(str->data); str->data = NULL; } void destroy_pr_append_str(pr_append_str *str) { if (str == NULL) return; destroy_pr_append_str_data(str); free(str); } int pr_append_external(pr_append_str *x, const char *s) { int xlen, slen; PR_ASSERT(NULL != s); PR_ASSERT(NULL != x); if (NULL == x->data) { x->storage_size = 24; x->data = (char *) malloc(x->storage_size); if (NULL == x->data) return 1; /* out of memory */ *x->data = '\0'; } xlen = strlen(x->data); slen = strlen(s); if (xlen + slen + 1 > x->storage_size) { x->storage_size += 2 * (slen + 1); x->data = (char *) realloc(x->data, x->storage_size); if (NULL == x->data) return 1; /* out of memory */ } strcpy(x->data + xlen, s); return 0; } static void pr_append_new_chunk(pr_append_str *x, const char *s) { PR_ASSERT(NULL != x) if (NULL == s) return; pr_append_w_sep(x, "; ", s); } int pr_append_new_chunk_external(pr_append_str *x, const char *s) { PR_ASSERT(NULL != x) if (NULL == s) return 0; return(pr_append_w_sep_external(x, "; ", s)); } int pr_append_w_sep_external(pr_append_str *x, const char *sep, const char *s) { PR_ASSERT(NULL != x) PR_ASSERT(NULL != s) PR_ASSERT(NULL != sep) if (pr_is_empty(x)) { return(pr_append_external(x, s)); } else { return(pr_append_external(x, sep) || pr_append_external(x, s)); } } void pr_set_empty(pr_append_str *x) { PR_ASSERT(NULL != x); if (NULL != x->data) *x->data = '\0'; } int pr_is_empty( const pr_append_str *x) { PR_ASSERT(NULL != x); return NULL == x->data || '\0' == *x->data; } static void pr_append_w_sep(pr_append_str *x, const char *sep, const char *s) { if (pr_append_w_sep_external(x, sep, s)) longjmp(_jmp_buf, 1); } static void pr_append(pr_append_str *x, const char *s) { if (pr_append_external(x, s)) longjmp(_jmp_buf, 1); } /* ============================================================ */ /* END internal and external functions for pr_append_str */ /* ============================================================ */ /* =========================================================== */ /* Malloc and realloc wrappers that longjmp() on failure */ /* =========================================================== */ static void * pr_safe_malloc(size_t x) { void *r = malloc(x); if (NULL == r) longjmp(_jmp_buf, 1); return r; } static void * pr_safe_realloc(void *p, size_t x) { void *r = realloc(p, x); if (NULL == r) longjmp(_jmp_buf, 1); return r; } /* =========================================================== */ /* End of malloc/realloc wrappers. */ /* =========================================================== */ /* ============================================================ */ /* START functions which check and modify the input */ /* ============================================================ */ /* Fuction to set the included region and fix the start positions */ static void _adjust_seq_args(const p3_global_settings *pa, seq_args *sa, pr_append_str *nonfatal_err, pr_append_str *warning) { int seq_len, inc_len; /* Create a seq for check primers if needed */ if (pa->primer_task == check_primers) { if (NULL == sa->sequence) { fake_a_sequence(sa, pa); } } if (pa->primer_task == pick_sequencing_primers && sa->incl_l != -1) { pr_append_new_chunk(nonfatal_err, "Task pick_sequencing_primers cannot be combined with included region"); return; } /* Complain if there is no sequence; We need to check this error here, because this function cannot do its work if sa->sequence == NULL */ if (NULL == sa->sequence) { if (pa->primer_task == check_primers) { pr_append_new_chunk(nonfatal_err, "No primers provided"); } else { pr_append_new_chunk(nonfatal_err, "Missing SEQUENCE tag"); } return; } seq_len = strlen(sa->sequence); /* For pick_cloning_primers set the forced positions */ if (pa->primer_task == pick_cloning_primers) { if(sa->incl_l == -1) { sa->force_left_start = pa->first_base_index; sa->force_right_start = seq_len + pa->first_base_index - 1; } else { sa->force_left_start = sa->incl_s; sa->force_right_start = sa->incl_s + sa->incl_l - 1; } } /* For pick_discriminative_primers set the forced positions */ if (pa->primer_task == pick_discriminative_primers) { /* Changed here from incl_s and incl_l to sa->tar2->pairs[0][0/1] */ if (sa->tar2.count != 1) { pr_append_new_chunk(nonfatal_err, "Task pick_discriminative_primers requires exactly one SEQUENCE_TARGET"); } sa->force_left_end = sa->tar2.pairs[0][0]; sa->force_right_end = sa->tar2.pairs[0][0] + sa->tar2.pairs[0][1] - 1; } /* If no included region is specified, * use the whole sequence as included region */ if (sa->incl_l == -1) { sa->incl_l = seq_len; sa->incl_s = pa->first_base_index; } /* Generate at least one target */ if (pa->primer_task == pick_sequencing_primers && sa->tar2.count == 0) { sa->tar2.pairs[0][0] = pa->first_base_index; sa->tar2.pairs[0][1] = seq_len; sa->tar2.count = 1; } /* Fix the start of the included region and start codon */ sa->incl_s -= pa->first_base_index; sa->start_codon_pos -= pa->first_base_index; /* Fix the start */ sa->force_left_start -= pa->first_base_index; sa->force_left_end -= pa->first_base_index; sa->force_right_start -= pa->first_base_index; sa->force_right_end -= pa->first_base_index; /* Make it relative to included region */ sa->force_left_start -= sa->incl_s; sa->force_left_end -= sa->incl_s; sa->force_right_start -= sa->incl_s; sa->force_right_end -= sa->incl_s; inc_len = sa->incl_s + sa->incl_l - 1; if ((sa->incl_l < INT_MAX) && (sa->incl_s > -1) && (sa->incl_l > -1) && (inc_len < seq_len) ) { /* Copies inluded region into trimmed_seq */ sa->trimmed_seq = (char *) pr_safe_malloc(sa->incl_l + 1); _pr_substr(sa->sequence, sa->incl_s, sa->incl_l, sa->trimmed_seq); /* Copies inluded region into trimmed_orig_seq */ /* edited by T. Koressaar for lowercase masking */ sa->trimmed_orig_seq = (char *) pr_safe_malloc(sa->incl_l + 1); _pr_substr(sa->sequence, sa->incl_s, sa->incl_l, sa->trimmed_orig_seq); /* Copies the whole sequence into upcased_seq */ sa->upcased_seq = (char *) pr_safe_malloc(strlen(sa->sequence) + 1); strcpy(sa->upcased_seq, sa->sequence); dna_to_upper(sa->upcased_seq, 1); /* We do not need to check for illegal characters in the return from dna_to_upper(), because errors are checked in _pr_data_control sa->trimmed_seq. */ /* Copies the reverse complement of the whole sequence into upcased_seq_r */ sa->upcased_seq_r = (char *) pr_safe_malloc(strlen(sa->sequence) + 1); p3_reverse_complement(sa->upcased_seq, sa->upcased_seq_r); } if (_check_and_adjust_intervals(sa, seq_len, pa->first_base_index, nonfatal_err, warning)) { return; } if (_check_and_adjust_overlap_pos(sa, sa->primer_overlap_junctions, &sa->primer_overlap_junctions_count, "SEQUENCE_OVERLAP_JUNCTION_LIST", seq_len, pa->first_base_index, nonfatal_err, warning)) { return; } /* Update ok regions, if non empty */ if (sa->ok_regions.count > 0) { _optimize_ok_regions_list(pa, sa); } } /* * This function uses the max/min product size info and the max/min * oligo length in order to reduce the ranges of the ok regions. On * some imputs this improves speed dramatically. */ static void _optimize_ok_regions_list(const p3_global_settings *pa, seq_args *sa) { /* We do this only if we enabled the optimization and * the primers were NOT specified. */ if (!OPTIMIZE_OK_REGIONS || (sa->left_input) || (sa->right_input)) { return; } /* If any pair is allowed, no point in doing this */ if (sa->ok_regions.any_pair) { return; } int pmin = INT_MAX; int pmax = 0; int omin = pa->p_args.min_size; int omax = pa->p_args.max_size; /* Determine min/max product size */ for (int i=0; inum_intervals; i++) { if (pa->pr_min[i] < pmin) { pmin = pa->pr_min[i]; } if (pa->pr_max[i] > pmax) { pmax = pa->pr_max[i]; } } /* Update each region */ for (int i=0; iok_regions.count; i++) { int ls = -1, le = -1, rs = -1, re = -1; int new_ls = -1, new_le = -1, new_rs = -1, new_re = -1; if (sa->ok_regions.left_pairs[i][0] != -1) { ls = sa->ok_regions.left_pairs[i][0]; le = sa->ok_regions.left_pairs[i][0] + sa->ok_regions.left_pairs[i][1] - 1; } if (sa->ok_regions.right_pairs[i][0] != -1) { rs = sa->ok_regions.right_pairs[i][0]; re = sa->ok_regions.right_pairs[i][0] + sa->ok_regions.right_pairs[i][1] - 1; } /* Compute new right region based on left range and min/max values of product size and oligo length */ if (ls != -1) { new_rs = ls + pmin - omax - 1; /* -1 just to be safe */ new_re = le - omin + pmax + 1; /* +1 just to be safe */ /* Adjust the ranges */ if ((rs == -1) || (new_rs > rs)) { rs = new_rs; } if ((re == -1) || (new_re < re)) { re = new_re; } if (rs < 0) { rs = 0; } if (re > (signed) strlen(sa->sequence)) { re = strlen(sa->sequence); } } /* Compute new left region based on right range and min/max values of product size and oligo length */ if (rs != -1) { new_ls = rs + omin - pmax - 1; /* -1 just to be safe */ new_le = re - pmin + omax + 1; /* +1 just to be safe */ /* Adjust the ranges */ if ((ls == -1) || (new_ls > ls)) { ls = new_ls; } if ((le == -1) || (new_le < le)) { le = new_le; } if (ls < 0) { ls = 0; } if (le > (signed) strlen(sa->sequence)) { le = strlen(sa->sequence); } } /* Temporary testing fprintf: */ /* fprintf(stderr, "Adjusted range [%d,%d,%d,%d] to [%d,%d,%d,%d], pmin is %d, pmax is %d, omin is %d, omax is %d\n", sa->ok_regions.left_pairs[i][0], sa->ok_regions.left_pairs[i][0] + sa->ok_regions.left_pairs[i][1] - 1, sa->ok_regions.right_pairs[i][0], sa->ok_regions.right_pairs[i][0] + sa->ok_regions.right_pairs[i][1] - 1, ls, le, rs, re, pmin, pmax, omin, omax); */ sa->ok_regions.left_pairs[i][0] = ls; sa->ok_regions.left_pairs[i][1] = le - ls + 1; sa->ok_regions.right_pairs[i][0] = rs; sa->ok_regions.right_pairs[i][1] = re - rs + 1; } /* any_left and any_right not true anymore */ sa->ok_regions.any_left = 0; sa->ok_regions.any_right = 0; } /* * Return 1 on error, 0 on success. fake_a_sequence creates a sequence * out of the provided primers and puts them in sa. */ static int fake_a_sequence(seq_args *sa, const p3_global_settings *pa) { int i, product_size, space, ns_to_fill; char *rev = NULL; int ns_to_fill_first, ns_to_fill_second; /* Determine the product size */ if ( pa->product_opt_size == PR_UNDEFINED_INT_OPT){ product_size = pa->pr_max[0] - pa->pr_min[0]; } else { product_size = pa->product_opt_size; } space = product_size + 1; ns_to_fill = product_size; /* Calculate how many Ns have to be added */ if (sa->left_input){ ns_to_fill = ns_to_fill - strlen(sa->left_input); } if (sa->right_input){ ns_to_fill = ns_to_fill - strlen(sa->right_input); rev = (char *) pr_safe_malloc(strlen(sa->right_input) + 1); p3_reverse_complement(sa->right_input, rev); } if (sa->internal_input){ ns_to_fill = ns_to_fill - strlen(sa->internal_input); } /* Return if there are no primers provided */ if (ns_to_fill == product_size + 1){ return 0; } ns_to_fill_first = ns_to_fill / 2; ns_to_fill_second = ns_to_fill - ns_to_fill_first; /* Allocate the space for the sequence */ sa->sequence = (char *) pr_safe_malloc(space); *sa->sequence = '\0'; /* Copy over the primers */ if (sa->left_input){ strcat(sa->sequence, sa->left_input); } /* Add the Ns*/ for (i = 0; i < ns_to_fill_first; i++ ) { strcat(sa->sequence, "N\0"); } if (sa->internal_input){ strcat(sa->sequence, sa->internal_input); } for (i = 0; i < ns_to_fill_second; i++ ) { strcat(sa->sequence, "N\0"); } if (sa->right_input){ strcat(sa->sequence, rev); } free(rev); return 0; } /* * Return 1 on error, 0 on success. Set sa->trimmed_seq and possibly modify * sa->tar. Upcase and check all bases in sa->trimmed_seq. * TO DO -- this would probably be cleaner if it only * checked, rather than updated, sa. */ /* Check if the input in sa and pa makes sense */ int _pr_data_control(const p3_global_settings *pa, const seq_args *sa, pr_append_str *glob_err, pr_append_str *nonfatal_err, pr_append_str *warning) { static char s1[MAX_PRIMER_LENGTH+1]; int i, pr_min, seq_len; char offending_char = '\0'; seq_len = strlen(sa->sequence); /* If sequence quality is provided, is it as long as the sequence? */ if (sa->n_quality !=0 && sa->n_quality != seq_len) pr_append_new_chunk(nonfatal_err, "Error in sequence quality data"); if ((pa->min_left_three_prime_distance < -1) || (pa->min_right_three_prime_distance < -1)) pr_append_new_chunk(nonfatal_err, "Minimum 3' distance must be >= -1 " "(min_*_three_prime_distance)"); if ((pa->p_args.min_quality != 0 || pa->o_args.min_quality != 0) && sa->n_quality == 0) pr_append_new_chunk(nonfatal_err, "Sequence quality data missing"); if (pa->first_base_index < PR_NULL_FORCE_POSITION) { pr_append_new_chunk(glob_err, "Value too small at tag PRIMER_FIRST_BASE_INDEX"); return 1; } if (pa->p_args.max_template_mispriming > SHRT_MAX && pa->thermodynamic_template_alignment == 0) { pr_append_new_chunk(glob_err, "Value too large at tag PRIMER_MAX_TEMPLATE_MISPRIMING"); return 1; } if (pa->pair_max_template_mispriming > SHRT_MAX && pa->thermodynamic_template_alignment == 0) { pr_append_new_chunk(glob_err, "Value too large at tag PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING"); return 1; } if (pa->p_args.max_repeat_compl > SHRT_MAX && pa->thermodynamic_oligo_alignment == 0) { pr_append_new_chunk(glob_err, "Value too large at tag PRIMER_MAX_LIBRARY_MISPRIMING"); return 1; } if (pa->o_args.max_repeat_compl > SHRT_MAX && pa->thermodynamic_oligo_alignment == 0) { pr_append_new_chunk(glob_err, "Value too large at tag PRIMER_INTERNAL_MAX_LIBRARY_MISHYB"); return 1; } if (pa->pair_repeat_compl > SHRT_MAX && pa->thermodynamic_oligo_alignment == 0) { pr_append_new_chunk(glob_err, "Value too large at tag PRIMER_PAIR_MAX_LIBRARY_MISPRIMING"); return 1; } if (pa->o_args.max_template_mispriming >= 0 && pa->thermodynamic_template_alignment==0) pr_append_new_chunk(glob_err, "PRIMER_INTERNAL_MAX_TEMPLATE_MISHYB is not supported"); if (pa->o_args.max_template_mispriming_th >= 0 && pa->thermodynamic_template_alignment==1) pr_append_new_chunk(glob_err, "PRIMER_INTERNAL_MAX_TEMPLATE_MISHYB_TH is not supported"); if (pa->p_args.min_size < 1) pr_append_new_chunk(glob_err, "PRIMER_MIN_SIZE must be >= 1"); if (pa->p_args.max_size > MAX_PRIMER_LENGTH) { pr_append_new_chunk(glob_err, "PRIMER_MAX_SIZE exceeds built-in maximum of "); pr_append(glob_err, MACRO_VALUE_AS_STRING(MAX_PRIMER_LENGTH)); return 1; } if (pa->p_args.opt_size > pa->p_args.max_size) { pr_append_new_chunk(glob_err, "PRIMER_{OPT,DEFAULT}_SIZE > PRIMER_MAX_SIZE"); return 1; } if (pa->p_args.opt_size < pa->p_args.min_size) { pr_append_new_chunk(glob_err, "PRIMER_{OPT,DEFAULT}_SIZE < PRIMER_MIN_SIZE"); return 1; } if (pa->o_args.max_size > MAX_PRIMER_LENGTH) { pr_append_new_chunk(glob_err, "PRIMER_INTERNAL_MAX_SIZE exceeds built-in maximum"); return 1; } if (pa->o_args.opt_size > pa->o_args.max_size) { pr_append_new_chunk(glob_err, "PRIMER_INTERNAL_{OPT,DEFAULT}_SIZE > MAX_SIZE"); return 1; } if (pa->o_args.opt_size < pa->o_args.min_size) { pr_append_new_chunk(glob_err, "PRIMER_INTERNAL_{OPT,DEFAULT}_SIZE < MIN_SIZE"); return 1; } /* A GC clamp can not be bigger then the primer */ if (pa->gc_clamp > pa->p_args.min_size) { pr_append_new_chunk(glob_err, "PRIMER_GC_CLAMP > PRIMER_MIN_SIZE"); return 1; } /* Must be >= 0 and <= 5 */ if ((pa->max_end_gc < 0) || (pa->max_end_gc > 5)) { pr_append_new_chunk(glob_err, "PRIMER_MAX_END_GC must be between 0 to 5"); return 1; } /* Product size must be provided */ if (0 == pa->num_intervals) { pr_append_new_chunk( glob_err, "Empty value for PRIMER_PRODUCT_SIZE_RANGE"); return 1; } for (i = 0; i < pa->num_intervals; i++) { if (pa->pr_min[i] > pa->pr_max[i] || pa->pr_min[i] < 0) { pr_append_new_chunk(glob_err, "Illegal element in PRIMER_PRODUCT_SIZE_RANGE"); return 1; } } pr_min = INT_MAX; /* Check if the primer is bigger then the product */ for(i=0;inum_intervals;i++) if(pa->pr_min[i]pr_min[i]; if (pa->p_args.max_size > pr_min) { pr_append_new_chunk(glob_err, "PRIMER_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE"); return 1; } if ((pa->pick_internal_oligo == 1 ) && pa->o_args.max_size > pr_min) { pr_append_new_chunk(glob_err, "PRIMER_INTERNAL_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE"); return 1; } /* There must be at least one primer returned */ if (pa->num_return < 1) { pr_append_new_chunk(glob_err, "PRIMER_NUM_RETURN < 1"); return 1; } if ((pa->p_args.must_match_five_prime != NULL) && (strlen(pa->p_args.must_match_five_prime) != 5)) { pr_append_new_chunk(glob_err, "PRIMER_MUST_MATCH_FIVE_PRIME must have 5 characters"); return 1; } if ((pa->p_args.must_match_three_prime != NULL) && (strlen(pa->p_args.must_match_three_prime) != 5)) { pr_append_new_chunk(glob_err, "PRIMER_MUST_MATCH_THREE_PRIME must have 5 characters"); return 1; } if ((pa->o_args.must_match_five_prime != NULL) && (strlen(pa->o_args.must_match_five_prime) != 5)) { pr_append_new_chunk(glob_err, "PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME must have 5 characters"); return 1; } if ((pa->o_args.must_match_three_prime != NULL) && (strlen(pa->o_args.must_match_three_prime) != 5)) { pr_append_new_chunk(glob_err, "PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME must have 5 characters"); return 1; } if (sa->incl_l >= INT_MAX) { pr_append_new_chunk(nonfatal_err, "Value for SEQUENCE_INCLUDED_REGION too large"); return 1; } if (sa->incl_s < 0 || sa->incl_l < 0 || sa->incl_s + sa->incl_l > seq_len) { pr_append_new_chunk(nonfatal_err, "Illegal value for SEQUENCE_INCLUDED_REGION"); return 1; } /* The product must fit in the included region */ if (sa->incl_l < pr_min && pa->pick_left_primer == 1 && pa->pick_right_primer == 1) { if (pa->primer_task == check_primers) { pr_append_new_chunk(warning, "SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE"); } else if (pa->primer_task != pick_primer_list) { pr_append_new_chunk(nonfatal_err, "SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE"); } if (pa->primer_task == generic) { return 1; } } if (pa->max_end_stability < 0) { pr_append_new_chunk(nonfatal_err, "PRIMER_MAX_END_STABILITY must be non-negative"); return 1; } /* Is the startodon ATG and in the incl. region */ if (!PR_START_CODON_POS_IS_NULL(sa)) { if (!PR_POSITION_PENALTY_IS_NULL(pa)) { pr_append_new_chunk(nonfatal_err, "Cannot accept both SEQUENCE_START_CODON_POSITION and non-default "); pr_append(nonfatal_err, "arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY"); } if (sa->start_codon_pos > (sa->incl_s + sa->incl_l - 3)) { pr_append_new_chunk(nonfatal_err, "Start codon position not contained in SEQUENCE_INCLUDED_REGION"); return 1; } else { if (sa->start_codon_pos >= 0 && ((sa->sequence[sa->start_codon_pos] != 'A' && sa->sequence[sa->start_codon_pos] != 'a') || (sa->sequence[sa->start_codon_pos + 1] != 'T' && sa->sequence[sa->start_codon_pos + 1] != 't') || (sa->sequence[sa->start_codon_pos + 2] != 'G' && sa->sequence[sa->start_codon_pos + 2] != 'g'))) { pr_append_new_chunk(nonfatal_err, "No start codon at SEQUENCE_START_CODON_POSITION"); return 1; } } } if (NULL != sa->quality) { if(pa->p_args.min_quality != 0 && pa->p_args.min_quality < pa->quality_range_min) { pr_append_new_chunk(glob_err, "PRIMER_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN"); return 1; } if (pa->p_args.min_quality != 0 && pa->p_args.min_quality > pa->quality_range_max) { pr_append_new_chunk(glob_err, "PRIMER_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX"); return 1; } if (pa->o_args.min_quality != 0 && pa->o_args.min_quality quality_range_min) { pr_append_new_chunk(glob_err, "PRIMER_INTERNAL_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN"); return 1; } if (pa->o_args.min_quality != 0 && pa->o_args.min_quality > pa->quality_range_max) { pr_append_new_chunk(glob_err, "PRIMER_INTERNAL_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX"); return 1; } for(i=0; i < sa->n_quality; i++) { if(sa->quality[i] < pa->quality_range_min || sa->quality[i] > pa->quality_range_max) { pr_append_new_chunk(nonfatal_err, "Sequence quality score out of range"); return 1; } } } else if (pa->p_args.weights.seq_quality || pa->o_args.weights.seq_quality) { pr_append_new_chunk(nonfatal_err, "Sequence quality is part of objective function but sequence quality is not defined"); return 1; } if ((offending_char = dna_to_upper(sa->trimmed_seq, 0))) { if (pa->liberal_base) { pr_append_new_chunk(warning, "Unrecognized base in input sequence"); } else { pr_append_new_chunk(nonfatal_err, "Unrecognized base in input sequence"); return 1; } } if (pa->p_args.opt_tm < pa->p_args.min_tm || pa->p_args.opt_tm > pa->p_args.max_tm) { pr_append_new_chunk(glob_err, "Optimum primer Tm lower than minimum or higher than maximum"); return 1; } if (pa->o_args.opt_tm < pa->o_args.min_tm || pa->o_args.opt_tm > pa->o_args.max_tm) { pr_append_new_chunk(glob_err, "Optimum internal oligo Tm lower than minimum or higher than maximum"); return 1; } if (pa->p_args.min_gc > pa->p_args.max_gc || pa->p_args.min_gc > 100 || pa->p_args.max_gc < 0){ pr_append_new_chunk(glob_err, "Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC"); return 1; } if (pa->o_args.min_gc > pa->o_args.max_gc || pa->o_args.min_gc > 100 || pa->o_args.max_gc < 0) { pr_append_new_chunk(glob_err, "Illegal value for PRIMER_INTERNAL_OLIGO_GC"); return 1; } if (pa->p_args.num_ns_accepted < 0) { pr_append_new_chunk(glob_err, "Illegal value for PRIMER_MAX_NS_ACCEPTED"); return 1; } if (pa->o_args.num_ns_accepted < 0){ pr_append_new_chunk(glob_err, "Illegal value for PRIMER_INTERNAL_MAX_NS_ACCEPTED"); return 1; } if (pa->p_args.max_self_any < 0 || pa->p_args.max_self_any > SHRT_MAX || pa->p_args.max_self_end < 0 || pa->p_args.max_self_end > SHRT_MAX || pa->pair_compl_any < 0 || pa->pair_compl_any > SHRT_MAX || pa->pair_compl_end < 0 || pa->pair_compl_end > SHRT_MAX) { pr_append_new_chunk(glob_err, "Illegal value for primer complementarity restrictions"); return 1; } if (pa->p_args.max_self_any_th < 0 || pa->p_args.max_self_end_th < 0 || pa->p_args.max_hairpin_th < 0 || pa->pair_compl_any_th < 0 || pa->pair_compl_end_th < 0) { pr_append_new_chunk(glob_err, "Illegal value for primer complementarity restrictions (thermod. approach)"); return 1; } if( pa->o_args.max_self_any < 0 || pa->o_args.max_self_any > SHRT_MAX || pa->o_args.max_self_end < 0 || pa->o_args.max_self_end > SHRT_MAX) { pr_append_new_chunk(glob_err, "Illegal value for internal oligo complementarity restrictions"); return 1; } if( pa->o_args.max_self_any_th < 0 || pa->o_args.max_self_end_th < 0 || pa->o_args.max_hairpin_th < 0) { pr_append_new_chunk(glob_err, "Illegal value for internal oligo complementarity restrictions"); return 1; } if (pa->p_args.salt_conc <= 0 || pa->p_args.dna_conc<=0){ pr_append_new_chunk(glob_err, "Illegal value for primer salt or dna concentration"); return 1; } if((pa->p_args.dntp_conc < 0 && pa->p_args.divalent_conc !=0 ) || pa->p_args.divalent_conc<0){ /* added by T.Koressaar */ pr_append_new_chunk(glob_err, "Illegal value for primer divalent salt or dNTP concentration"); return 1; } if(pa->o_args.salt_conc<=0||pa->o_args.dna_conc<=0){ pr_append_new_chunk(glob_err, "Illegal value for internal oligo salt or dna concentration"); return 1; } if((pa->o_args.dntp_conc<0 && pa->o_args.divalent_conc!=0) || pa->o_args.divalent_conc < 0) { /* added by T.Koressaar */ pr_append_new_chunk(glob_err, "Illegal value for internal oligo divalent salt or dNTP concentration"); return 1; } if (!_PR_DEFAULT_POSITION_PENALTIES(pa) && sa->tar2.count > 1) { pr_append_new_chunk(nonfatal_err, "Non-default inside penalty or outside penalty "); pr_append(nonfatal_err, "is valid only when number of targets <= 1"); } if (!_PR_DEFAULT_POSITION_PENALTIES(pa) && 0 == sa->tar2.count) { pr_append_new_chunk(warning, "Non-default inside penalty or outside penalty "); pr_append(warning, "has no effect when number of targets is 0"); } if (pa->pick_internal_oligo != 1 && sa->internal_input) { pr_append_new_chunk(nonfatal_err, "Not specified to pick internal oligos"); pr_append(nonfatal_err, " but a specific internal oligo is provided"); } if (sa->internal_input) { if (strlen(sa->internal_input) > MAX_PRIMER_LENGTH) { pr_append_new_chunk(glob_err, "Specified internal oligo exceeds built-in maximum of "); pr_append(glob_err, MACRO_VALUE_AS_STRING(MAX_PRIMER_LENGTH)); return 1; } if (strlen(sa->internal_input) > (unsigned) pa->o_args.max_size) pr_append_new_chunk(warning, "Specified internal oligo > PRIMER_INTERNAL_MAX_SIZE"); if (strlen(sa->internal_input) < (unsigned) pa->o_args.min_size) pr_append_new_chunk(warning, "Specified internal oligo < PRIMER_INTERNAL_MIN_SIZE"); if (!strstr_nocase(sa->sequence, sa->internal_input)) pr_append_new_chunk(nonfatal_err, "Specified internal oligo not in sequence"); else if (!strstr_nocase(sa->trimmed_seq, sa->internal_input)) pr_append_new_chunk(nonfatal_err, "Specified internal oligo not in Included Region"); } if (sa->left_input) { if (strlen(sa->left_input) > MAX_PRIMER_LENGTH) { pr_append_new_chunk(glob_err, "Specified left primer exceeds built-in maximum of "); pr_append(glob_err, MACRO_VALUE_AS_STRING(MAX_PRIMER_LENGTH)); return 1; } if (strlen(sa->left_input) > (unsigned) pa->p_args.max_size) pr_append_new_chunk(warning, "Specified left primer > PRIMER_MAX_SIZE"); if (strlen(sa->left_input) < (unsigned) pa->p_args.min_size) pr_append_new_chunk(warning, "Specified left primer < PRIMER_MIN_SIZE"); if (!strstr_nocase(sa->sequence, sa->left_input)) pr_append_new_chunk(nonfatal_err, "Specified left primer not in sequence"); else if (!strstr_nocase(sa->trimmed_seq, sa->left_input)) pr_append_new_chunk(nonfatal_err, "Specified left primer not in Included Region"); } if (sa->right_input) { if (strlen(sa->right_input) > MAX_PRIMER_LENGTH) { pr_append_new_chunk(glob_err, "Specified right primer exceeds built-in maximum of "); pr_append(glob_err, MACRO_VALUE_AS_STRING(MAX_PRIMER_LENGTH)); return 1; } if (strlen(sa->right_input) < (unsigned) pa->p_args.min_size) pr_append_new_chunk(warning, "Specified right primer < PRIMER_MIN_SIZE"); if (strlen(sa->right_input) > (unsigned) pa->p_args.max_size) { pr_append_new_chunk(warning, "Specified right primer > PRIMER_MAX_SIZE"); } else { /* We do not want to overflow s1. */ p3_reverse_complement(sa->right_input,s1); if (!strstr_nocase(sa->sequence, s1)) pr_append_new_chunk(nonfatal_err, "Specified right primer not in sequence"); else if (!strstr_nocase(sa->trimmed_seq, s1)) pr_append_new_chunk(nonfatal_err, "Specified right primer not in Included Region"); } } if ((pa->pr_pair_weights.product_tm_lt || pa->pr_pair_weights.product_tm_gt) && pa->product_opt_tm == PR_UNDEFINED_DBL_OPT) { pr_append_new_chunk(glob_err, "Product temperature is part of objective function while optimum temperature is not defined"); return 1; } if((pa->pr_pair_weights.product_size_lt || pa->pr_pair_weights.product_size_gt) && pa->product_opt_size == PR_UNDEFINED_INT_OPT){ pr_append_new_chunk(glob_err, "Product size is part of objective function while optimum size is not defined"); return 1; } if ((pa->p_args.weights.gc_content_lt || pa->p_args.weights.gc_content_gt) && pa->p_args.opt_gc_content == DEFAULT_OPT_GC_PERCENT) { pr_append_new_chunk(glob_err, "Primer GC content is part of objective function while optimum gc_content is not defined"); return 1; } if ((pa->o_args.weights.gc_content_lt || pa->o_args.weights.gc_content_gt) && pa->o_args.opt_gc_content == DEFAULT_OPT_GC_PERCENT) { pr_append_new_chunk(glob_err, "Hyb probe GC content is part of objective function " "while optimum gc_content is not defined"); return 1; } if ((pa->pick_internal_oligo != 1) && (pa->pr_pair_weights.io_quality)) { pr_append_new_chunk(glob_err, "Internal oligo quality is part of objective function " "while internal oligo choice is not required"); return 1; } if (pa->p_args.weights.repeat_sim && (!seq_lib_num_seq(pa->p_args.repeat_lib))) { pr_append_new_chunk(glob_err, "Mispriming score is part of objective function, but mispriming library is not defined"); return 1; } if (pa->o_args.weights.repeat_sim && (!seq_lib_num_seq(pa->o_args.repeat_lib))) { pr_append_new_chunk(glob_err, "Internal oligo mispriming score is part of objective function while mishyb library is not defined"); return 1; } if (pa->pr_pair_weights.repeat_sim && (!(seq_lib_num_seq(pa->p_args.repeat_lib)))) { pr_append_new_chunk(glob_err, "Mispriming score is part of objective function, " "but mispriming library is not defined"); return 1; } if(pa->pr_pair_weights.io_quality && pa->pick_internal_oligo == 0 ) { pr_append_new_chunk(glob_err, "Internal oligo quality is part of objective function" " while internal oligo choice is not required"); return 1; } if (pa->sequencing.lead < 0) { pr_append_new_chunk(glob_err, "Illegal value for PRIMER_SEQUENCING_LEAD"); return 1; } if (pa->sequencing.interval < 0) { pr_append_new_chunk(glob_err, "Illegal value for PRIMER_SEQUENCING_INTERVAL"); return 1; } if (pa->sequencing.accuracy < 0) { pr_append_new_chunk(glob_err, "Illegal value for PRIMER_SEQUENCING_ACCURACY"); return 1; } if (pa->sequencing.spacing < 0) { pr_append_new_chunk(glob_err, "Illegal value for PRIMER_SEQUENCING_SPACING"); return 1; } if(pa->sequencing.interval > pa->sequencing.spacing) { pr_append_new_chunk(glob_err, "PRIMER_SEQUENCING_INTERVAL > PRIMER_SEQUENCING_SPACING"); return 1; } if(pa->sequencing.accuracy > pa->sequencing.spacing) { pr_append_new_chunk(glob_err, "PRIMER_SEQUENCING_ACCURACY > PRIMER_SEQUENCING_SPACING"); return 1; } if(pa->sequencing.lead > pa->sequencing.spacing) { pr_append_new_chunk(glob_err, "PRIMER_SEQUENCING_LEAD > PRIMER_SEQUENCING_SPACING"); return 1; } if((sa->force_left_start > -1) && (sa->force_left_end > -1) && (sa->force_left_start > sa->force_left_end)) { pr_append_new_chunk(glob_err, "SEQUENCE_FORCE_LEFT_START > SEQUENCE_FORCE_LEFT_END"); return 1; } if((sa->force_right_end > -1) && (sa->force_right_start > -1) && (sa->force_right_end > sa->force_right_start)) { pr_append_new_chunk(glob_err, "SEQUENCE_FORCE_RIGHT_END > SEQUENCE_FORCE_RIGHT_START"); return 1; } if (pa->min_5_prime_overlap_of_junction < 1) { pr_append_new_chunk(glob_err, "Illegal value for PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION"); return 1; } if (pa->min_3_prime_overlap_of_junction < 1) { pr_append_new_chunk(glob_err, "Illegal value for PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION"); return 1; } if ((sa->primer_overlap_junctions_count > 0) && (pa->min_5_prime_overlap_of_junction > (pa->p_args.max_size / 2))) { pr_append_new_chunk(glob_err, "PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION > PRIMER_MAX_SIZE / 2"); return 1; } if ((sa->primer_overlap_junctions_count > 0) && (pa->min_3_prime_overlap_of_junction > (pa->p_args.max_size / 2))) { pr_append_new_chunk(glob_err, "PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION > PRIMER_MAX_SIZE / 2"); return 1; } if (pa->p_args.divalent_conc > 0.0 && pa->p_args.dntp_conc <= 0.0) { pr_append_new_chunk(warning, "PRIMER_SALT_DIVALENT > 0.0 " "but PRIMER_DNTP_CONC <= 0.0; " "use reasonable value for PRIMER_DNTP_CONC"); } if ((pa->p_args.must_match_five_prime != NULL) && (test_must_match_parameters(pa->p_args.must_match_five_prime))) { pr_append_new_chunk(glob_err, "Illegal values for PRIMER_MUST_MATCH_FIVE_PRIME"); return 1; } if ((pa->p_args.must_match_three_prime != NULL) && (test_must_match_parameters(pa->p_args.must_match_three_prime))) { pr_append_new_chunk(glob_err, "Illegal values for PRIMER_MUST_MATCH_THREE_PRIME"); return 1; } if ((pa->o_args.must_match_five_prime != NULL) && (test_must_match_parameters(pa->o_args.must_match_five_prime))) { pr_append_new_chunk(glob_err, "Illegal values for PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME"); return 1; } if ((pa->o_args.must_match_three_prime != NULL) && (test_must_match_parameters(pa->o_args.must_match_three_prime))) { pr_append_new_chunk(glob_err, "Illegal values for PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME"); return 1; } return (NULL == nonfatal_err->data && NULL == glob_err->data) ? 0 : 1; } /* _pr_data_control */ static int _check_and_adjust_overlap_pos(seq_args *sa, int *list, int *count, const char *tag, int seq_len, int first_index, pr_append_str *nonfatal_err, pr_append_str *warning) { int i; int outside_warning_issued = 0; char buffer[255]; if (*count == 0) { return 0; } for (i = 0; i < *count; i++) { /* Subtract first_index from the "must overlap" positions */ list[i] -= first_index; if (list[i] >= seq_len) { sprintf(buffer, "%s beyond end of sequence", tag); pr_append_new_chunk(nonfatal_err, buffer); return 1; } if (list[i] < 0) { sprintf(buffer, "Negative %s length", tag); pr_append_new_chunk(nonfatal_err, buffer); return 1; } /* Cause the intron positions to be relative to the included region. */ list[i] -= sa->incl_s; /* Check that intron positions are within the included region. */ if (list[i] < 0 || list[i] > sa->incl_l) { if (!outside_warning_issued) { sprintf(buffer, "%s outside of INCLUDED_REGION", tag); pr_append_new_chunk(warning, buffer); outside_warning_issued = 1; } } } return 0; } static int _check_and_adjust_intervals(seq_args *sa, int seq_len, int first_index, pr_append_str * nonfatal_err, pr_append_str *warning) { if (_check_and_adjust_1_interval("TARGET", sa->tar2.count, sa->tar2.pairs, seq_len, first_index, nonfatal_err, sa, warning, 0) == 1) return 1; sa->start_codon_pos -= sa->incl_s; if (_check_and_adjust_1_interval("EXCLUDED_REGION", sa->excl2.count, sa->excl2.pairs, seq_len, first_index, nonfatal_err, sa, warning, 0) == 1) return 1; if (_check_and_adjust_1_interval("PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION", sa->excl_internal2.count, sa->excl_internal2.pairs, seq_len, first_index, nonfatal_err, sa, warning, 0) == 1) return 1; if (_check_and_adjust_1_interval("PRIMER_PAIR_OK_REGION_LIST", sa->ok_regions.count, sa->ok_regions.left_pairs, seq_len, first_index, nonfatal_err, sa, warning, 1) == 1) return 1; if (_check_and_adjust_1_interval("PRIMER_PAIR_OK_REGION_LIST", sa->ok_regions.count, sa->ok_regions.right_pairs, seq_len, first_index, nonfatal_err, sa, warning, 1) == 1) return 1; return 0; } /* * Check intervals, and add any errors to err. * Update the start of each interval to * be relative to the start of the included region. */ static int _check_and_adjust_1_interval(const char *tag_name, int num_intervals, interval_array_t intervals, int seq_len, int first_index, pr_append_str *err, seq_args *sa, pr_append_str *warning, int empty_allowed) { int i; int outside_warning_issued = 0; /* Subtract the first_index from the start positions in the interval array */ for (i = 0; i < num_intervals; i++) { if (empty_allowed && (intervals[i][0] == -1) && (intervals[i][1] == -1)) continue; if (empty_allowed && (((intervals[i][0] == -1) && (intervals[i][1] != -1)) || ((intervals[i][0] != -1) && (intervals[i][1] == -1)))) { pr_append_new_chunk(err, tag_name); pr_append(err, " illegal interval"); return 1; } intervals[i][0] -= first_index; } for (i=0; i < num_intervals; i++) { if (empty_allowed && (intervals[i][0] == -1) && (intervals[i][1] == -1)) continue; if (intervals[i][0] + intervals[i][1] > seq_len) { pr_append_new_chunk(err, tag_name); pr_append(err, " beyond end of sequence"); return 1; } /* Cause the interval start to be relative to the included region. */ intervals[i][0] -= sa->incl_s; /* Check that intervals are within the included region. */ if (intervals[i][0] < 0 || intervals[i][0] + intervals[i][1] > sa->incl_l) { if (!outside_warning_issued) { pr_append_new_chunk(warning, tag_name); pr_append(warning, " outside of INCLUDED_REGION"); outside_warning_issued = 1; } } if (intervals[i][1] < 0) { pr_append_new_chunk(err, "Negative "); pr_append(err, tag_name); pr_append(err, " length"); return 1; } } return 0; } /* _check_and_adjust_intervals */ /* ============================================================ */ /* END functions that check and modify the input */ /* ============================================================ */ /* ============================================================ */ /* BEGIN create primer files functions */ /* ============================================================ */ /* START OF WHAT SHOULD GO TO IO_PRIMER_FILES.C */ /* Creates up to three files, the names of which are based on the argument 'file_name'. One file is a table of forward primers, one a table of reverse primers, and one a table of internal hybridization oligos, depending on what the caller to choose_primers() requested (in the 'pa' argument). Returns 0 on success, 1 on error. On error, check errno for ENOMEM. Used to implement P3_FILE_FLAG=1. */ int p3_print_oligo_lists(const p3retval *retval, const seq_args *sa, const p3_global_settings *pa, pr_append_str *err, const char *file_name) { /* Figure out if the sequence starts at position 1 or 0 */ int first_base_index = pa->first_base_index; int ret; /* Start building up a filename */ char *file; FILE *fh; if (setjmp(_jmp_buf) != 0) { return 1; /* If we get here, that means we returned via a longjmp. In this case errno should be ENOMEM. */ } file = (char *) malloc(strlen(sa->sequence_name) + 5); if (NULL == file) return 1; /* ENOMEM */ /* Check if the left primers have to be printed */ if( pa->pick_left_primer ) { /* Create the file name and open file*/ strcpy(file, sa->sequence_name); strcat(file, ".for"); if (!(fh = fopen(file,"w"))) { if (pr_append_new_chunk_external(err, "Unable to open file ")) return 1; if (pr_append_external(err, file)) return 1; if (pr_append_external(err, " for writing")) return 1; free(file); return 1; } /* Print the content to the file */ ret = p3_print_one_oligo_list(sa, retval->fwd.num_elem, retval->fwd.oligo, OT_LEFT, first_base_index, NULL != pa->p_args.repeat_lib, fh,pa->thermodynamic_oligo_alignment); fclose(fh); if (ret) return 1; } /* Check if the right primers have to be printed */ if (pa->pick_right_primer) { strcpy(file, sa->sequence_name); strcat(file, ".rev"); if (!(fh = fopen(file,"w"))) { pr_append_new_chunk(err, "Unable to open file "); pr_append(err, file); pr_append(err, " for writing"); free(file); return 1; } /* Print the content to the file */ ret = p3_print_one_oligo_list(sa, retval->rev.num_elem, retval->rev.oligo, OT_RIGHT, first_base_index, NULL != pa->p_args.repeat_lib, fh, pa->thermodynamic_oligo_alignment); fclose(fh); if (ret) return 1; } /* Check if the internal oligos have to be printed */ if (pa->pick_internal_oligo) { /* Create the file name and open file*/ strcpy(file, sa->sequence_name); strcat(file, ".int"); if (!(fh = fopen(file,"w"))) { if (pr_append_new_chunk_external(err, "Unable to open file ")) return 1; if (pr_append_external(err, file)) return 1; if (pr_append_external(err, " for writing")) return 1; free(file); return 1; } /* Print the content to the file */ ret = p3_print_one_oligo_list(sa, retval->intl.num_elem, retval->intl.oligo, OT_INTL, first_base_index, NULL != pa->o_args.repeat_lib, fh, pa->thermodynamic_oligo_alignment); fclose(fh); if (ret) return 1; } free(file); return 0; } /* Print out the content of one primer array */ /* Return 1 on error, otherwise 0. */ int p3_print_one_oligo_list(const seq_args *sa, int n, const primer_rec *oligo_arr, const oligo_type o_type, const int first_base_index, const int print_lib_sim, FILE *fh, const int thermodynamic_oligo_alignment) { int i; /* Print out the header for the table */ if (print_list_header(fh, o_type, first_base_index, print_lib_sim, thermodynamic_oligo_alignment)) return 1; /* error */ /* Iterate over the array */ for (i = 0; i < n; i++) { /* Print each single oligo */ if (print_oligo(fh, sa, i, &oligo_arr[i], o_type, first_base_index, print_lib_sim, thermodynamic_oligo_alignment)) return 1; /* error */ } return 0; /* success */ } static int print_list_header(FILE *fh, oligo_type type, int first_base_index, int print_lib_sim, int thermodynamic_oligo_alignment) { int ret; ret = fprintf(fh, "ACCEPTABLE %s\n", OT_LEFT == type ? "LEFT PRIMERS" : OT_RIGHT == type ? "RIGHT PRIMERS" : "INTERNAL OLIGOS"); if (ret < 0) return 1; ret = fprintf(fh, " %4d-based ", first_base_index); if (ret < 0) return 1; if(thermodynamic_oligo_alignment) ret = fprintf(fh, "# self self hair-"); else ret = fprintf(fh, "# self self"); if (ret < 0) return 1; if (print_lib_sim) /* ret = fprintf(fh, "# self self lib qual-\n"); */ ret = fprintf(fh, " lib"); if (ret < 0) return 1; ret = fprintf(fh, " qual-\n"); if (ret < 0) return 1; ret = fprintf(fh, " # sequence start ln "); if (ret < 0) return 1; ret = fprintf(fh, "N GC%% Tm"); if (ret < 0) return 1; if(thermodynamic_oligo_alignment) ret = fprintf(fh, " any_th end_th pin"); else ret = fprintf(fh, " any end"); if (ret < 0) return 1; if (print_lib_sim) ret = fprintf(fh, " sim lity\n"); else ret = fprintf(fh, " lity\n"); if (ret < 0) return 1; return 0; } static int print_oligo(FILE *fh, const seq_args *sa, int index, const primer_rec *h, oligo_type type, int first_base_index, int print_lib_sim, int thermodynamical_approach) { int ret; /* WARNING, *p points to static storage that is overwritten on next call to pr_oligo_sequence or pr_oligo_rev_c_sequence. */ char *p = (OT_RIGHT != type) ? pr_oligo_sequence(sa, h) : pr_oligo_rev_c_sequence(sa, h); ret = fprintf(fh, "%4d %-30s %5d %2d %2d %5.2f %5.3f %5.2f %5.2f", index, p, h->start+sa->incl_s + first_base_index, h->length, h->num_ns, h->gc_content, h->temp, h->self_any, h->self_end); if (ret < 0) return 1; if (1==thermodynamical_approach) { ret = fprintf(fh, " %5.2f", h->hairpin_th); if (ret < 0) return 1; } if (ret < 0) return 1; if (print_lib_sim) { PR_ASSERT(h->repeat_sim.score != NULL); ret = fprintf(fh, " %5.2f", h->repeat_sim.score[h->repeat_sim.max]); if (ret < 0) return 1; } ret = fprintf(fh, " %6.3f\n", h->quality); if (ret < 0) return 1; else return 0; } /* END OF WHAT SHOULD GO TO IO_PRIMER_FILES.C */ /* ============================================================ */ /* END create primer files functions */ /* ============================================================ */ /* ============================================================ * BEGIN p3_read_line, a utility used * both for reading boulder input and for reading sequence * libraries. * ============================================================ */ /* Read a line of any length from file. Return NULL on end of file, * otherwise return a pointer to static storage containing the line. Any * trailing newline is stripped off. Be careful -- there is only * one static buffer, so subsequent calls will replace the string * pointed to by the return value. * Used in seq_lib functions and also exported * used in p3_read_line and seq_lib functions * */ #define INIT_BUF_SIZE 1024 char* p3_read_line(FILE *file) { static size_t ssz; static char *s = NULL; size_t remaining_size; char *p, *n; if (NULL == s) { ssz = INIT_BUF_SIZE; s = (char *) pr_safe_malloc(ssz); } p = s; remaining_size = ssz; while (1) { if (fgets(p, remaining_size, file) == NULL) /* End of file. */ return p == s ? NULL : s; if ((n = strchr(p, '\n')) != NULL) { *n = '\0'; return s; } /* We did not get the whole line. */ /* * The following assertion is a bit of hack, a at least for 32-bit * machines, because we will usually run out of address space first. * Really we should treat an over-long line as an input error, but * since an over-long line is unlikely and we do want to provide some * protection.... */ PR_ASSERT(ssz <= INT_MAX); if (ssz >= INT_MAX / 2) ssz = INT_MAX; else { ssz *= 2; } s = (char *) pr_safe_realloc(s, ssz); p = strchr(s, '\0'); remaining_size = ssz - (p - s); } } /* ============================================================ */ /* END p3_read_line */ /* ============================================================ */ /* ============================================================ */ /* BEGIN 'get' functions for seq_args */ /* ============================================================ */ const interval_array_t2 * p3_get_sa_tar2(const seq_args *sargs) { return &sargs->tar2 ; } const interval_array_t2 * p3_get_sa_excl2(const seq_args *sargs) { return &sargs->excl2 ; } const interval_array_t2 * p3_get_sa_excl_internal2(const seq_args *sargs) { return &sargs->excl_internal2 ; } const interval_array_t4 * p3_get_sa_ok_regions(const seq_args *sargs) { return &sargs->ok_regions; } const int* p3_get_sa_overlap_junctions(const seq_args *sargs) { return sargs->primer_overlap_junctions; } /* ============================================================ */ /* END 'get' functions for seq_args */ /* ============================================================ */ int p3_get_sa_n_quality(seq_args *sargs) { return sargs->n_quality ; } /* ============================================================ */ /* BEGIN 'set' functions for seq_args */ /* ============================================================ */ int p3_set_sa_sequence(seq_args *sargs, const char *sequence) { return _set_string(&sargs->sequence, sequence) ; } void p3_set_sa_primer_sequence_quality(seq_args *sargs, int quality) { sargs->quality[sargs->n_quality++] = quality ; } int p3_set_sa_sequence_name(seq_args *sargs, const char* sequence_name) { return _set_string(&sargs->sequence_name, sequence_name); } int p3_set_sa_left_input(seq_args *sargs, const char *s) { return _set_string(&sargs->left_input, s); } int p3_set_sa_right_input(seq_args *sargs, const char *s) { return _set_string(&sargs->right_input, s); } int p3_set_sa_internal_input(seq_args *sargs, const char *s) { return _set_string(&sargs->internal_input, s); } void p3_set_sa_incl_s(seq_args *sargs, int incl_s) { sargs->incl_s = incl_s; } void p3_set_sa_incl_l(seq_args *sargs, int incl_l) { sargs->incl_l = incl_l; } void p3_set_sa_empty_quality(seq_args *sargs) { sargs->n_quality = 0; } void p3_sa_add_to_quality_array(seq_args *sargs, int quality) { int n = sargs->n_quality; if (sargs->quality_storage_size == 0) { sargs->quality_storage_size = 3000; sargs->quality = (int *) pr_safe_malloc(sizeof(*sargs->quality) * sargs->quality_storage_size); } if (n > sargs->quality_storage_size) { sargs->quality_storage_size *= 2; sargs->quality = (int *) pr_safe_realloc(sargs->quality, sizeof(*sargs->quality) * sargs->quality_storage_size); } sargs->quality[n] = quality; sargs->n_quality++; } int p3_sa_add_to_overlap_junctions_array(seq_args *sargs, int overlap) { int c = sargs->primer_overlap_junctions_count; if (c >= PR_MAX_INTERVAL_ARRAY) return 1; sargs->primer_overlap_junctions[sargs->primer_overlap_junctions_count++] = overlap; return 0; } void p3_set_sa_start_codon_pos(seq_args *sargs, int start_codon_pos) { sargs->start_codon_pos = start_codon_pos; } int p3_add_to_sa_tar2(seq_args *sargs, int n1, int n2) { return p3_add_to_interval_array(&sargs->tar2, n1, n2); } int p3_add_to_sa_excl2(seq_args *sargs, int n1, int n2) { return p3_add_to_interval_array(&sargs->excl2, n1, n2); } int p3_add_to_sa_excl_internal2(seq_args *sargs, int n1, int n2) { return p3_add_to_interval_array(&sargs->excl_internal2, n1, n2); } int p3_add_to_sa_ok_regions(seq_args *sargs, int l1, int l2, int r1, int r2) { return p3_add_to_2_interval_array(&sargs->ok_regions, l1, l2, r1, r2); } /* ============================================================ */ /* END 'set' functions for seq_args */ /* ============================================================ */ /* ============================================================ */ /* BEGIN 'get' functions for p3_global_settings */ /* ============================================================ */ args_for_one_oligo_or_primer * p3_get_gs_p_args(p3_global_settings * p) { return &p->p_args; } args_for_one_oligo_or_primer * p3_get_gs_o_args(p3_global_settings * p) { return &p->o_args; } /* ============================================================ */ /* END 'get' functions for p3_global_settings */ /* ============================================================ */ /* ============================================================ */ /* BEGIN 'set' functions for p3_global_settings */ /* ============================================================ */ void p3_set_gs_prmin (p3_global_settings * p , int val, int i) { p->pr_min[i] = val ; } void p3_set_gs_prmax (p3_global_settings * p , int val, int i) { p->pr_max[i] = val ; } void p3_set_gs_primer_opt_size(p3_global_settings * p , int val) { p->p_args.opt_size = val ; } void p3_set_gs_primer_min_size(p3_global_settings * p , int val) { p->p_args.min_size = val ; } void p3_set_gs_primer_max_size(p3_global_settings * p , int val) { p->p_args.max_size = val ; } void p3_set_gs_primer_max_poly_x(p3_global_settings * p , int val) { p->p_args.max_poly_x = val; } void p3_set_gs_primer_opt_tm(p3_global_settings * p , double d) { p->p_args.opt_tm = d ; } void p3_set_gs_primer_opt_gc_percent(p3_global_settings * p , double d) { p->p_args.opt_gc_content = d ; } void p3_set_gs_primer_min_tm(p3_global_settings * p , double d) { p->p_args.min_tm = d ; } void p3_set_gs_primer_max_tm(p3_global_settings * p , double d) { p->p_args.max_tm = d; } void p3_set_gs_primer_max_diff_tm(p3_global_settings * p , double val) { p->max_diff_tm = val; } void p3_set_gs_primer_tm_santalucia(p3_global_settings * p, tm_method_type val) { p->tm_santalucia = val ; } void p3_set_gs_primer_salt_corrections(p3_global_settings * p, salt_correction_type val) { p->salt_corrections = val; } void p3_set_gs_primer_min_gc(p3_global_settings * p , double val) { p->p_args.min_gc = val ; } void p3_set_gs_primer_max_gc(p3_global_settings * p , double val) { p->p_args.max_gc = val ; } void p3_set_gs_primer_salt_conc(p3_global_settings * p , double val) { p->p_args.salt_conc = val ; } void p3_set_gs_primer_divalent_conc(p3_global_settings * p , double val) { p->p_args.divalent_conc = val; } void p3_set_gs_primer_dntp_conc(p3_global_settings * p , double val) { p->p_args.dntp_conc = val ; } void p3_set_gs_primer_dna_conc(p3_global_settings * p , double val) { p->p_args.dna_conc = val ; } void p3_set_gs_primer_num_ns_accepted(p3_global_settings * p , int val) { p->p_args.num_ns_accepted = val ; } void p3_set_gs_primer_product_opt_size(p3_global_settings * p , int val) { p->product_opt_size = val ; } void p3_set_gs_primer_self_any(p3_global_settings * p , double val) { p->p_args.max_self_any = val; } void p3_set_gs_primer_self_any_th(p3_global_settings * p , double val) { p->p_args.max_self_any_th = val; } void p3_set_gs_primer_self_end(p3_global_settings * p , double val) { p->p_args.max_self_end = val; /* (short) (val * 100); */ } void p3_set_gs_primer_self_end_th(p3_global_settings * p , double val) { p->p_args.max_self_end_th = val; } void p3_set_gs_primer_hairpin_th(p3_global_settings * p , double val) { p->p_args.max_hairpin_th = val; } void /* Called in primer3_boulder_main.c. */ p3_set_gs_primer_file_flag(p3_global_settings * p , int file_flag) { p->file_flag = file_flag; } void p3_set_gs_pick_anyway(p3_global_settings * p , int pick_anyway) { p->pick_anyway = pick_anyway ; } void p3_set_gs_gc_clamp(p3_global_settings * p , int gc_clamp) { p->gc_clamp = gc_clamp; } void p3_set_gs_primer_liberal_base(p3_global_settings * p , int val) { p->liberal_base = val; } void p3_set_gs_primer_first_base_index(p3_global_settings * p , int first_base_index){ p->first_base_index = first_base_index; } void p3_set_gs_primer_num_return(p3_global_settings * p , int val) { p->num_return = val ; } void p3_set_gs_primer_min_quality(p3_global_settings * p , int val) { p->p_args.min_quality = val ; } void p3_set_gs_primer_min_end_quality(p3_global_settings * p , int val) { p->p_args.min_end_quality = val ; } void p3_set_gs_primer_quality_range_min(p3_global_settings * p , int val) { p->quality_range_min = val ; } void p3_set_gs_primer_quality_range_max(p3_global_settings * p , int val) { p->quality_range_max = val ; } void p3_set_gs_primer_product_max_tm(p3_global_settings * p , double val) { p->product_max_tm = val ; } void p3_set_gs_primer_product_min_tm(p3_global_settings * p , double val) { p->product_min_tm = val ; } void p3_set_gs_primer_product_opt_tm(p3_global_settings * p , double val) { p->product_opt_tm = val ; } void p3_set_gs_primer_task(p3_global_settings * pa , char * task_tmp) { if (!strcmp_nocase(task_tmp, "pick_pcr_primers")) { pa->primer_task = generic; pa->pick_left_primer = 1; pa->pick_right_primer = 1; pa->pick_internal_oligo = 0; } else if (!strcmp_nocase(task_tmp, "pick_pcr_primers_and_hyb_probe")) { pa->primer_task = generic; pa->pick_left_primer = 1; pa->pick_right_primer = 1; pa->pick_internal_oligo = 1; } else if (!strcmp_nocase(task_tmp, "pick_left_only")) { pa->primer_task = generic; pa->pick_left_primer = 1; pa->pick_right_primer = 0; pa->pick_internal_oligo = 0; } else if (!strcmp_nocase(task_tmp, "pick_right_only")) { pa->primer_task = generic; pa->pick_left_primer = 0; pa->pick_right_primer = 1; pa->pick_internal_oligo = 0; } else if (!strcmp_nocase(task_tmp, "pick_hyb_probe_only")) { pa->primer_task = generic; pa->pick_left_primer = 0; pa->pick_right_primer = 0; pa->pick_internal_oligo = 1; } else if (!strcmp_nocase(task_tmp, "generic")) { pa->primer_task = generic; } else if (!strcmp_nocase(task_tmp, "pick_detection_primers")) { pa->primer_task = generic; /* Deliberate duplication for backward compatibility. */ } else if (!strcmp_nocase(task_tmp, "pick_cloning_primers")) { pa->primer_task = pick_cloning_primers; } else if (!strcmp_nocase(task_tmp, "pick_discriminative_primers")) { pa->primer_task = pick_discriminative_primers; } else if (!strcmp_nocase(task_tmp, "pick_sequencing_primers")) { pa->primer_task = pick_sequencing_primers; } else if (!strcmp_nocase(task_tmp, "pick_primer_list")) { pa->primer_task = pick_primer_list; } else if (!strcmp_nocase(task_tmp, "check_primers")) { pa->primer_task = check_primers; } } void p3_set_gs_primer_pick_right_primer(p3_global_settings * p , int pick_right_primer) { p->pick_right_primer = pick_right_primer; } void p3_set_gs_primer_pick_internal_oligo(p3_global_settings * p , int pick_internal_oligo) { p->pick_internal_oligo = pick_internal_oligo; } void p3_set_gs_primer_pick_left_primer(p3_global_settings * p , int pick_left_primer) { p->pick_left_primer = pick_left_primer; } void p3_set_gs_primer_internal_oligo_opt_size(p3_global_settings * p , int val) { p->o_args.opt_size = val ; } void p3_set_gs_primer_internal_oligo_max_size(p3_global_settings * p , int val) { p->o_args.max_size = val ; } void p3_set_gs_primer_internal_oligo_min_size(p3_global_settings * p , int val) { p->o_args.min_size = val ; } void p3_set_gs_primer_internal_oligo_max_poly_x(p3_global_settings * p , int val) { p->o_args.max_poly_x = val ; } void p3_set_gs_primer_internal_oligo_opt_tm(p3_global_settings * p , double val) { p->o_args.opt_tm = val ; } void p3_set_gs_primer_internal_oligo_opt_gc_percent(p3_global_settings * p , double val) { p->o_args.opt_gc_content = val ; } void p3_set_gs_primer_internal_oligo_max_tm(p3_global_settings * p , double val) { p->o_args.max_tm = val ; } void p3_set_gs_primer_internal_oligo_min_tm(p3_global_settings * p , double val) { p->o_args.min_tm = val ; } void p3_set_gs_primer_internal_oligo_min_gc(p3_global_settings * p , double val) { p->o_args.min_gc = val ; } void p3_set_gs_primer_internal_oligo_max_gc(p3_global_settings * p , double val) { p->o_args.max_gc = val ; } void p3_set_gs_primer_internal_oligo_salt_conc(p3_global_settings * p , double val) { p->o_args.salt_conc = val ; } void p3_set_gs_primer_internal_oligo_divalent_conc(p3_global_settings * p , double val) { p->o_args.divalent_conc = val ; } void p3_set_gs_primer_internal_oligo_dntp_conc(p3_global_settings * p , double val) { p->o_args.dntp_conc = val ; } void p3_set_gs_primer_internal_oligo_dna_conc(p3_global_settings * p , double val) { p->o_args.dna_conc = val ; } void p3_set_gs_primer_internal_oligo_num_ns(p3_global_settings * p , int val) { p->o_args.num_ns_accepted = val ; } void p3_set_gs_primer_internal_oligo_min_quality(p3_global_settings * p , int val) { p->o_args.min_quality = val ; } void p3_set_gs_primer_internal_oligo_self_any(p3_global_settings * p , double val) { p->o_args.max_self_any = val; } void p3_set_gs_primer_internal_oligo_self_any_th(p3_global_settings * p , double val) { p->o_args.max_self_any_th = val; } void p3_set_gs_primer_internal_oligo_self_end(p3_global_settings * p , double val) { p->o_args.max_self_end = val; /* (short) (val * 100); */ } void p3_set_gs_primer_internal_oligo_self_end_th(p3_global_settings * p , double val) { p->o_args.max_self_end_th = val; } void p3_set_gs_primer_internal_oligo_hairpin_th(p3_global_settings * p , double val) { p->o_args.max_hairpin_th = val; } void p3_set_gs_primer_max_mispriming(p3_global_settings * p , double val) { p->p_args.max_repeat_compl = val; } void p3_set_gs_primer_internal_oligo_max_mishyb(p3_global_settings * p , double val) { p->o_args.max_repeat_compl = val; } void p3_set_gs_primer_pair_max_mispriming(p3_global_settings * p , double val) { p->pair_repeat_compl = val; } void p3_set_gs_primer_max_template_mispriming(p3_global_settings * p , double val) { p->p_args.max_template_mispriming = val; } void p3_set_gs_primer_max_template_mispriming_th(p3_global_settings * p , double val) { p->p_args.max_template_mispriming_th = val; } void p3_set_gs_primer_lib_ambiguity_codes_consensus(p3_global_settings * p , int val) { p->lib_ambiguity_codes_consensus = val ; } void p3_set_gs_primer_inside_penalty(p3_global_settings * p , double val) { p->inside_penalty = val ; } void p3_set_gs_primer_outside_penalty(p3_global_settings * p , double val) { p->outside_penalty = val ; } void p3_set_gs_primer_mispriming_library(p3_global_settings * p , char * path) { /* TO DO: Re-factor this? TO DO: Check for errors */ p->p_args.repeat_lib = read_and_create_seq_lib(path, "mispriming library") ; } void p3_set_gs_primer_internal_oligo_mishyb_library(p3_global_settings * p , char * path) { /* TO DO: Re-factor this? TO DO: Check for errors */ p->o_args.repeat_lib = read_and_create_seq_lib(path, "internal oligio mishyb library") ; } void p3_set_gs_primer_max_end_stability(p3_global_settings * p , double val) { p->max_end_stability = val ; } void p3_set_gs_primer_lowercase_masking(p3_global_settings * p , int val) { p->lowercase_masking = val ; } void p3_set_gs_primer_thermodynamic_oligo_alignment(p3_global_settings * p , int val) { p->thermodynamic_oligo_alignment = val ; } void p3_set_gs_primer_thermodynamic_template_alignment(p3_global_settings * p , int val) { p->thermodynamic_template_alignment = val ; } void p3_set_gs_primer_wt_tm_gt(p3_global_settings * p , double val) { p->p_args.weights.temp_gt = val ; } void p3_set_gs_primer_wt_tm_lt(p3_global_settings * p , double val) { p->p_args.weights.temp_lt = val ; } void p3_set_gs_primer_wt_gc_percent_gt(p3_global_settings * p , double val) { p->p_args.weights.gc_content_gt = val ; } void p3_set_gs_primer_wt_gc_percent_lt(p3_global_settings * p , double val) { p->p_args.weights.gc_content_lt = val ; } void p3_set_gs_primer_wt_size_lt(p3_global_settings * p , double val) { p->p_args.weights.length_lt = val ; } void p3_set_gs_primer_wt_size_gt(p3_global_settings * p , double val) { p->p_args.weights.length_gt = val ; } void p3_set_gs_primer_wt_compl_any(p3_global_settings * p , double val) { p->p_args.weights.compl_any = val ; } void p3_set_gs_primer_wt_compl_any_th(p3_global_settings * p , double val) { p->p_args.weights.compl_any_th = val ; } void p3_set_gs_primer_wt_compl_end(p3_global_settings * p , double val) { p->p_args.weights.compl_end = val ; } void p3_set_gs_primer_wt_compl_end_th(p3_global_settings * p , double val) { p->p_args.weights.compl_end_th = val ; } void p3_set_gs_primer_wt_hairpin_th(p3_global_settings * p , double val) { p->p_args.weights.hairpin_th = val ; } void p3_set_gs_primer_wt_num_ns(p3_global_settings * p , double val) { p->p_args.weights.num_ns = val ; } void p3_set_gs_primer_wt_rep_sim(p3_global_settings * p , double val) { p->p_args.weights.repeat_sim = val ; } void p3_set_gs_primer_wt_seq_qual(p3_global_settings * p , double val) { p->p_args.weights.seq_quality = val ; } void p3_set_gs_primer_wt_end_qual(p3_global_settings * p , double val) { p->p_args.weights.end_quality = val ; } void p3_set_gs_primer_wt_pos_penalty(p3_global_settings * p , double val){ p->p_args.weights.pos_penalty = val ; } void p3_set_gs_primer_wt_end_stability(p3_global_settings * p , double val) { p->p_args.weights.end_stability = val ; } void p3_set_gs_primer_wt_template_mispriming(p3_global_settings * p , double val) { p->p_args.weights.template_mispriming = val ; } void p3_set_gs_primer_wt_template_mispriming_th(p3_global_settings * p , double val) { p->p_args.weights.template_mispriming_th = val ; } void p3_set_gs_primer_io_wt_tm_gt(p3_global_settings * p , double val) { p->o_args.weights.temp_gt = val ; } void p3_set_gs_primer_io_wt_tm_lt(p3_global_settings * p , double val){ p->o_args.weights.temp_lt = val ; } void p3_set_gs_primer_io_wt_gc_percent_gt(p3_global_settings * p , double val) { p->o_args.weights.gc_content_gt = val ; } void p3_set_gs_primer_io_wt_gc_percent_lt(p3_global_settings * p , double val) { p->o_args.weights.gc_content_lt = val ; } void p3_set_gs_primer_io_wt_size_lt(p3_global_settings * p , double val) { p->o_args.weights.length_lt = val ; } void p3_set_gs_primer_io_wt_size_gt(p3_global_settings * p , double val) { p->o_args.weights.length_gt = val ; } void p3_set_gs_primer_io_wt_wt_compl_any(p3_global_settings * p , double val) { p->o_args.weights.compl_any = val ; } void p3_set_gs_primer_io_wt_wt_compl_any_th(p3_global_settings * p , double val) { p->o_args.weights.compl_any_th = val ; } void p3_set_gs_primer_io_wt_compl_end(p3_global_settings * p , double val) { p->o_args.weights.compl_end = val ; } void p3_set_gs_primer_io_wt_compl_end_th(p3_global_settings * p , double val) { p->o_args.weights.compl_end_th = val ; } void p3_set_gs_primer_io_wt_hairpin_th(p3_global_settings * p , double val) { p->o_args.weights.hairpin_th = val ; } void p3_set_gs_primer_io_wt_num_ns(p3_global_settings * p , double val) { p->o_args.weights.num_ns = val ; } void p3_set_gs_primer_io_wt_rep_sim(p3_global_settings * p , double val) { p->o_args.weights.repeat_sim = val ; } void p3_set_gs_primer_io_wt_seq_qual(p3_global_settings * p , double val) { p->o_args.weights.seq_quality = val ; } void p3_set_gs_primer_io_wt_end_qual(p3_global_settings * p , double val) { p->o_args.weights.end_quality = val ; } void p3_set_gs_primer_pair_wt_pr_penalty(p3_global_settings * p , double val) { p->pr_pair_weights.primer_quality = val ; } void p3_set_gs_primer_pair_wt_io_penalty(p3_global_settings * p , double val) { p->pr_pair_weights.io_quality = val ; } void p3_set_gs_primer_pair_wt_diff_tm(p3_global_settings * p , double val) { p->pr_pair_weights.diff_tm = val ; } void p3_set_gs_primer_pair_wt_compl_any(p3_global_settings * p , double val) { p->pr_pair_weights.compl_any = val ; } void p3_set_gs_primer_pair_wt_compl_any_th(p3_global_settings * p , double val) { p->pr_pair_weights.compl_any_th = val ; } void p3_set_gs_primer_pair_wt_compl_end(p3_global_settings * p , double val) { p->pr_pair_weights.compl_end = val ; } void p3_set_gs_primer_pair_wt_compl_end_th(p3_global_settings * p , double val) { p->pr_pair_weights.compl_end_th = val ; } void p3_set_gs_primer_pair_wt_product_tm_lt(p3_global_settings * p , double val) { p->pr_pair_weights.product_tm_lt = val ; } void p3_set_gs_primer_pair_wt_product_tm_gt(p3_global_settings * p , double val) { p->pr_pair_weights.product_tm_gt = val ; } void p3_set_gs_primer_pair_wt_product_size_gt(p3_global_settings * p , double val) { p->pr_pair_weights.product_size_gt = val ; } void p3_set_gs_primer_pair_wt_product_size_lt(p3_global_settings * p , double val) { p->pr_pair_weights.product_size_lt = val ; } void p3_set_gs_primer_pair_wt_rep_sim(p3_global_settings * p , double val) { p->pr_pair_weights.repeat_sim = val ; } void p3_set_gs_primer_pair_wt_template_mispriming(p3_global_settings * p , double val) { p->pr_pair_weights.template_mispriming = val ; } void p3_set_gs_primer_pair_wt_template_mispriming_th(p3_global_settings * p , double val){ p->pr_pair_weights.template_mispriming_th = val ; } void p3_set_gs_lib_ambiguity_codes_consensus(p3_global_settings * p, int lib_ambiguity_codes_consensus) { p->lib_ambiguity_codes_consensus = lib_ambiguity_codes_consensus; } void p3_set_gs_quality_range_min(p3_global_settings * p , int quality_range_min){ p->quality_range_min = quality_range_min; } void p3_set_gs_quality_range_max(p3_global_settings * p , int quality_range_max){ p->quality_range_max = quality_range_max ; } void p3_set_gs_max_end_gc(p3_global_settings *p, int max_end_gc) { p->max_end_gc = max_end_gc; } void p3_set_gs_max_end_stability(p3_global_settings * p , int max_end_stability){ p->max_end_stability = max_end_stability; } void p3_set_gs_lowercase_masking(p3_global_settings * p , int lowercase_masking){ p->lowercase_masking = lowercase_masking; } void p3_set_gs_outside_penalty(p3_global_settings * p , double outside_penalty){ p->outside_penalty = outside_penalty; } void p3_set_gs_inside_penalty(p3_global_settings * p , double inside_penalty){ p->inside_penalty = inside_penalty; } void p3_set_gs_pair_max_template_mispriming(p3_global_settings * p, double pair_max_template_mispriming) { p->pair_max_template_mispriming = pair_max_template_mispriming; } void p3_set_gs_pair_max_template_mispriming_th(p3_global_settings * p, double pair_max_template_mispriming_th) { p->pair_max_template_mispriming_th = pair_max_template_mispriming_th; } void p3_set_gs_pair_repeat_compl(p3_global_settings * p, double pair_repeat_compl) { p->pair_repeat_compl = pair_repeat_compl; } void p3_set_gs_pair_compl_any(p3_global_settings * p , double pair_compl_any) { p->pair_compl_any = pair_compl_any; } void p3_set_gs_pair_compl_any_th(p3_global_settings * p , double pair_compl_any_th) { p->pair_compl_any_th = pair_compl_any_th; } void p3_set_gs_pair_compl_end(p3_global_settings * p , double pair_compl_end) { p->pair_compl_end = pair_compl_end; } void p3_set_gs_pair_compl_end_th(p3_global_settings * p , double pair_compl_end_th) { p->pair_compl_end = pair_compl_end_th; } void p3_set_gs_min_left_three_prime_distance(p3_global_settings *p, int min_distance) { p->min_left_three_prime_distance = min_distance; } void p3_set_gs_min_right_three_prime_distance(p3_global_settings *p, int min_distance) { p->min_right_three_prime_distance = min_distance; } void p3_set_gs_min_5_prime_overlap_of_junction(p3_global_settings *p, int min_5_prime) { p->min_5_prime_overlap_of_junction = min_5_prime; } void p3_set_gs_min_3_prime_overlap_of_junction(p3_global_settings *p, int min_3_prime) { p->min_3_prime_overlap_of_junction = min_3_prime; } void p3_set_gs_max_diff_tm(p3_global_settings * p , double max_diff_tm) { p->max_diff_tm = max_diff_tm; } void p3_set_gs_primer_pick_anyway(p3_global_settings * p , int val) { p->pick_anyway = val ; } void p3_set_gs_primer_gc_clamp(p3_global_settings * p , int val) { p->gc_clamp = val; } void p3_empty_gs_product_size_range(p3_global_settings *pgs) { pgs->num_intervals = 0; } int p3_add_to_gs_product_size_range(p3_global_settings *pgs, int n1, int n2) { if (pgs->num_intervals >= PR_MAX_INTERVAL_ARRAY) return 1; pgs->pr_min[pgs->num_intervals] = n1; pgs->pr_max[pgs->num_intervals] = n2; pgs->num_intervals++; return 0; } /* ============================================================ */ /* END 'set' functions for p3_global_settings */ /* ============================================================ */ /* ============================================================ */ /* START functions for setting and getting oligo problems */ /* ============================================================ */ static void initialize_op(primer_rec *oligo) { oligo->problems.prob = 0UL; /* 0UL is the unsigned long zero */ } /* We use bitfields to store all primer data. The idea is that * a primer is uninitialized if all bits are 0. It was evaluated * if OP_PARTIALLY_WRITTEN is true. If OP_COMPLETELY_WRITTEN is * also true the primer was evaluated till the end - meaning if * all OP_... are false, the primer is OK, if some are true * primer3 was forced to use it (must_use). */ #define OP_PARTIALLY_WRITTEN (1UL << 0) #define OP_COMPLETELY_WRITTEN (1UL << 1) #define BF_OVERLAPS_TARGET (1UL << 2) #define BF_OVERLAPS_EXCL_REGION (1UL << 3) #define BF_INFINITE_POSITION_PENALTY (1UL << 4) /* Space for more bitfields */ #define OP_NOT_IN_ANY_OK_REGION (1UL << 7) #define OP_TOO_MANY_NS (1UL << 8) /* 3prime problem*/ #define OP_OVERLAPS_TARGET (1UL << 9) /* 3prime problem*/ #define OP_HIGH_GC_CONTENT (1UL << 10) #define OP_LOW_GC_CONTENT (1UL << 11) #define OP_HIGH_TM (1UL << 12) #define OP_LOW_TM (1UL << 13) #define OP_OVERLAPS_EXCL_REGION (1UL << 14) /* 3prime problem*/ #define OP_HIGH_SELF_ANY (1UL << 15) /* 3prime problem*/ #define OP_HIGH_SELF_END (1UL << 16) #define OP_NO_GC_CLAMP (1UL << 17) /* 3prime problem*/ #define OP_HIGH_END_STABILITY (1UL << 18) /* 3prime problem*/ #define OP_HIGH_POLY_X (1UL << 19) /* 3prime problem*/ #define OP_LOW_SEQUENCE_QUALITY (1UL << 20) /* 3prime problem*/ #define OP_LOW_END_SEQUENCE_QUALITY (1UL << 21) /* 3prime problem*/ #define OP_HIGH_SIM_TO_NON_TEMPLATE_SEQ (1UL << 22) /* 3prime problem*/ #define OP_HIGH_SIM_TO_MULTI_TEMPLATE_SITES (1UL << 23) #define OP_OVERLAPS_MASKED_SEQ (1UL << 24) #define OP_TOO_LONG (1UL << 25) /* 3prime problem*/ #define OP_TOO_SHORT (1UL << 26) #define OP_DOES_NOT_AMPLIFY_ORF (1UL << 27) #define OP_TOO_MANY_GC_AT_END (1UL << 28) /* 3prime problem*/ #define OP_HIGH_HAIRPIN (1UL << 29) /* 3prime problem*/ #define OP_MUST_MATCH_ERR (1UL << 30) /* Space for more Errors */ /* Tip: the calculator of windows (in scientific mode) can easily convert between decimal and binary numbers */ /* all bits 1 except bits 0 to 6 */ /* (~0UL) ^ 127UL = 1111 1111 1111 1111 1111 1111 1000 0000 */ static unsigned long int any_problem = (~0UL) ^ 127UL; /* 310297344UL = 0001 0010 0111 1110 1100 0011 0000 0000 */ static unsigned long int five_prime_problem = 310297344UL; int p3_ol_is_uninitialized(const primer_rec *oligo) { return (oligo->problems.prob == 0UL); } int p3_ol_is_ok(const primer_rec *oligo) { return (oligo->problems.prob & OP_COMPLETELY_WRITTEN) != 0; } /* Return 1 iff the argument 'oligo' has any problems -- i.e. violations of design constraints. */ int p3_ol_has_any_problem(const primer_rec *oligo) { return (oligo->problems.prob & any_problem) != 0; } static int any_5_prime_ol_extension_has_problem(const primer_rec *oligo) { return (oligo->problems.prob & five_prime_problem) != 0; } /* Return a string details the the problems in 'oligo', i.e. the constraints that 'oligo' violates. WARNING: Returns a pointer to static storage, which is over-written on next call. */ #define ADD_OP_STR(COND, STR) if (prob & COND) { tmp = STR; strcpy(next, tmp); next += strlen(tmp); } const char * p3_get_ol_problem_string(const primer_rec *oligo) { static char output[4096*2]; /* WARNING, make sure all error strings concatenated will not overflow this buffer. */ char *next = output; const char *tmp; unsigned long int prob = oligo->problems.prob; output[0] = '\0'; if ( (prob & OP_PARTIALLY_WRITTEN) && !(prob & OP_COMPLETELY_WRITTEN) ) { tmp = " Not completely checked;"; strcpy(next, tmp); next += strlen(tmp); } { ADD_OP_STR(OP_TOO_MANY_NS, " Too many Ns;") ADD_OP_STR(OP_OVERLAPS_TARGET, " Overlaps target;") ADD_OP_STR(OP_HIGH_GC_CONTENT, " GC content too high;") ADD_OP_STR(OP_LOW_GC_CONTENT, " GC content too low;") ADD_OP_STR(OP_HIGH_TM, " Temperature too high;") ADD_OP_STR(OP_LOW_TM, " Temperature too low;") ADD_OP_STR(OP_OVERLAPS_EXCL_REGION, " Overlaps an excluded region;") ADD_OP_STR(OP_NOT_IN_ANY_OK_REGION, " Not in any ok region;") ADD_OP_STR(OP_HIGH_SELF_ANY, " Similarity to self too high;") ADD_OP_STR(OP_HIGH_SELF_END, " Similary to 3' end of self too high;") ADD_OP_STR(OP_HIGH_HAIRPIN, " Hairpin stability too high;") ADD_OP_STR(OP_NO_GC_CLAMP, " No 3' GC clamp;") ADD_OP_STR(OP_TOO_MANY_GC_AT_END, " Too many GCs at 3' end;") ADD_OP_STR(OP_HIGH_END_STABILITY, " 3' end too stable (delta-G too high);") ADD_OP_STR(OP_HIGH_POLY_X, " Contains too-long poly nucleotide tract;") ADD_OP_STR(OP_LOW_SEQUENCE_QUALITY, " Template sequence quality too low;") ADD_OP_STR(OP_LOW_END_SEQUENCE_QUALITY, " Template sequence quality at 3' end too low;") ADD_OP_STR(OP_HIGH_SIM_TO_NON_TEMPLATE_SEQ, " Similarity to non-template sequence too high;") ADD_OP_STR(OP_HIGH_SIM_TO_MULTI_TEMPLATE_SITES, " Similarity to multiple sites in template;") ADD_OP_STR(OP_OVERLAPS_MASKED_SEQ, " 3' base overlaps masked sequence;") ADD_OP_STR(OP_TOO_LONG, " Too long;") ADD_OP_STR(OP_TOO_SHORT, " Too short;") ADD_OP_STR(OP_DOES_NOT_AMPLIFY_ORF, " Would not amplify an open reading frame;") ADD_OP_STR(OP_MUST_MATCH_ERR, " Failed must_match requirements;") } return output; } #undef ADD_OP_STR static void bf_set_overlaps_target(primer_rec *oligo, int val){ if (val == 0) { oligo->problems.prob |= BF_OVERLAPS_TARGET; oligo->problems.prob ^= BF_OVERLAPS_TARGET; } else { oligo->problems.prob |= BF_OVERLAPS_TARGET; } } static int bf_get_overlaps_target(const primer_rec *oligo) { return (oligo->problems.prob & BF_OVERLAPS_TARGET) != 0; } static void bf_set_overlaps_excl_region(primer_rec *oligo, int val){ if (val == 0) { oligo->problems.prob |= BF_OVERLAPS_EXCL_REGION; oligo->problems.prob ^= BF_OVERLAPS_EXCL_REGION; } else { oligo->problems.prob |= BF_OVERLAPS_EXCL_REGION; } } static int bf_get_overlaps_excl_region(const primer_rec *oligo) { return (oligo->problems.prob & BF_OVERLAPS_EXCL_REGION) != 0; } static void bf_set_infinite_pos_penalty(primer_rec *oligo, int val){ if (val == 0) { oligo->problems.prob |= BF_INFINITE_POSITION_PENALTY; oligo->problems.prob ^= BF_INFINITE_POSITION_PENALTY; } else { oligo->problems.prob |= BF_INFINITE_POSITION_PENALTY; } } static int bf_get_infinite_pos_penalty(const primer_rec *oligo) { return (oligo->problems.prob & BF_INFINITE_POSITION_PENALTY) != 0; } static void op_set_does_not_amplify_orf(primer_rec *oligo) { oligo->problems.prob |= OP_DOES_NOT_AMPLIFY_ORF; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_completely_written(primer_rec *oligo) { oligo->problems.prob |= OP_COMPLETELY_WRITTEN; } static void op_set_must_match_err(primer_rec *oligo) { oligo->problems.prob |= OP_MUST_MATCH_ERR; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_too_many_ns(primer_rec *oligo) { oligo->problems.prob |= OP_TOO_MANY_NS; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_overlaps_target(primer_rec *oligo) { oligo->problems.prob |= OP_OVERLAPS_TARGET; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_high_gc_content(primer_rec *oligo) { oligo->problems.prob |= OP_HIGH_GC_CONTENT; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_low_gc_content(primer_rec *oligo) { oligo->problems.prob |= OP_LOW_GC_CONTENT; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_high_tm(primer_rec *oligo) { oligo->problems.prob |= OP_HIGH_TM; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_low_tm(primer_rec *oligo) { oligo->problems.prob |= OP_LOW_TM; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_overlaps_excluded_region(primer_rec *oligo) { oligo->problems.prob |= OP_OVERLAPS_EXCL_REGION; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_not_in_any_ok_region(primer_rec *oligo) { oligo->problems.prob |= OP_NOT_IN_ANY_OK_REGION; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_high_self_any(primer_rec *oligo) { oligo->problems.prob |= OP_HIGH_SELF_ANY; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_high_self_end(primer_rec *oligo) { oligo->problems.prob |= OP_HIGH_SELF_END; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_high_hairpin_th(primer_rec *oligo) { oligo->problems.prob |= OP_HIGH_HAIRPIN; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_no_gc_glamp(primer_rec *oligo) { oligo->problems.prob |= OP_NO_GC_CLAMP; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_too_many_gc_at_end(primer_rec *oligo) { oligo->problems.prob |= OP_TOO_MANY_GC_AT_END; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } /* Must not be called on a hybridization probe / internal oligo */ static void op_set_high_end_stability(primer_rec *oligo) { oligo->problems.prob |= OP_HIGH_END_STABILITY; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_high_poly_x(primer_rec *oligo) { oligo->problems.prob |= OP_HIGH_POLY_X; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_low_sequence_quality(primer_rec *oligo) { oligo->problems.prob |= OP_LOW_SEQUENCE_QUALITY; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_low_end_sequence_quality(primer_rec *oligo) { oligo->problems.prob |= OP_LOW_END_SEQUENCE_QUALITY; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_high_similarity_to_non_template_seq(primer_rec *oligo) { oligo->problems.prob |= OP_HIGH_SIM_TO_NON_TEMPLATE_SEQ; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_high_similarity_to_multiple_template_sites(primer_rec *oligo) { oligo->problems.prob |= OP_HIGH_SIM_TO_MULTI_TEMPLATE_SITES; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_overlaps_masked_sequence(primer_rec *oligo) { oligo->problems.prob |= OP_OVERLAPS_MASKED_SEQ; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_too_long(primer_rec *oligo) { oligo->problems.prob |= OP_TOO_LONG; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } static void op_set_too_short(primer_rec *oligo) { oligo->problems.prob |= OP_TOO_SHORT; oligo->problems.prob |= OP_PARTIALLY_WRITTEN; } /* ============================================================ */ /* END functions for setting and getting oligo problems */ /* ============================================================ */ /* ============================================================ */ /* START functions for getting values from p3retvals */ /* ============================================================ */ char * p3_get_rv_and_gs_warnings(const p3retval *retval, const p3_global_settings *pa) { pr_append_str warning; PR_ASSERT(NULL != pa); init_pr_append_str(&warning); if (seq_lib_warning_data(pa->p_args.repeat_lib)) pr_append_new_chunk(&warning, seq_lib_warning_data(pa->p_args.repeat_lib)); if(seq_lib_warning_data(pa->o_args.repeat_lib)) { pr_append_new_chunk(&warning, seq_lib_warning_data(pa->o_args.repeat_lib)); pr_append(&warning, " (for internal oligo)"); } if (!pr_is_empty(&retval->warnings)) pr_append_new_chunk(&warning, retval->warnings.data); return pr_is_empty(&warning) ? NULL : warning.data; } const char * p3_get_rv_global_errors(const p3retval *retval) { return retval->glob_err.data; } const char * p3_get_rv_per_sequence_errors(const p3retval *retval) { return retval->per_sequence_err.data; } p3_output_type p3_get_rv_output_type(const p3retval *r) { return r->output_type; } const char * p3_get_rv_warnings(const p3retval *r) { return pr_append_str_chars(&r->warnings); } int p3_get_rv_stop_codon_pos(p3retval *r) { return r->stop_codon_pos; } /* ============================================================ */ /* END functions for getting values from p3retvals */ /* ============================================================ */ void p3_print_args(const p3_global_settings *p, seq_args *s) { int i; if (p != NULL) { printf("=============\n"); printf("BEGIN GLOBAL ARGS\n") ; printf(" primer_task %i\n", p->primer_task); printf(" pick_left_primer %i\n", p->pick_left_primer); printf(" pick_right_primer %i\n", p->pick_right_primer); printf(" pick_internal_oligo %i\n", p->pick_internal_oligo); printf(" file_flag %i\n", p->file_flag) ; printf(" first_base_index %i\n", p->first_base_index); printf(" liberal_base %i\n", p->liberal_base ); printf(" num_return %i\n", p->num_return) ; printf(" pick_anyway %i\n", p->pick_anyway); printf(" lib_ambiguity_codes_consensus %i\n", p->lib_ambiguity_codes_consensus) ; printf(" quality_range_min %i\n", p->quality_range_min) ; printf(" quality_range_max %i\n", p->quality_range_max) ; printf(" tm_santalucia %i\n", p->tm_santalucia) ; printf(" salt_corrections %i\n", p->salt_corrections) ; printf(" max_end_stability %f\n", p->max_end_stability) ; printf(" gc_clamp %i\n", p->gc_clamp) ; printf(" max_end_gc %i\n", p->max_end_gc); printf(" lowercase_masking %i\n", p->lowercase_masking) ; printf(" thermodynamic_oligo_alignment %i\n", p->thermodynamic_oligo_alignment); printf(" thermodynamic_template_alignment %i\n", p->thermodynamic_template_alignment); printf(" outside_penalty %f\n", p->outside_penalty) ; printf(" inside_penalty %f\n", p->inside_penalty) ; printf(" number of product size ranges: %d\n", p->num_intervals); printf(" product size ranges:\n"); for (i = 0; i < p->num_intervals; i++) { printf(" %d - %d \n", p->pr_min[i], p->pr_max[i]); } printf(" product_opt_size %i\n", p->product_opt_size) ; printf(" product_max_tm %f\n", p->product_max_tm) ; printf(" product_min_tm %f\n", p->product_min_tm) ; printf(" product_opt_tm %f\n", p->product_opt_tm) ; printf(" pair_max_template_mispriming %f\n", p->pair_max_template_mispriming) ; printf(" pair_max_template_mispriming_th %f\n", p->pair_max_template_mispriming_th) ; printf(" pair_repeat_compl %f\n", p->pair_repeat_compl) ; printf(" pair_compl_any %f\n", p->pair_compl_any) ; printf(" pair_compl_end %f\n", p->pair_compl_end) ; printf(" pair_compl_any_th %f\n", p->pair_compl_any_th) ; printf(" pair_compl_end_th %f\n", p->pair_compl_end_th) ; printf(" min_left_three_prime_distance %i\n", p->min_left_three_prime_distance) ; printf(" min_right_three_prime_distance %i\n", p->min_right_three_prime_distance) ; printf(" min_5_prime_overlap_of_junction %i\n", p->min_5_prime_overlap_of_junction); printf(" min_3_prime_overlap_of_junction %i\n", p->min_3_prime_overlap_of_junction); printf(" dump %i\n", p->dump); printf(" begin pr_pair_weights\n") ; printf(" primer_quality %f\n", p->pr_pair_weights.primer_quality) ; printf(" io_quality %f\n", p->pr_pair_weights.io_quality) ; printf(" diff_tm %f\n", p->pr_pair_weights.diff_tm) ; printf(" compl_any %f\n", p->pr_pair_weights.compl_any) ; printf(" compl_end %f\n", p->pr_pair_weights.compl_end) ; printf(" compl_any_th %f\n", p->pr_pair_weights.compl_any_th) ; printf(" compl_end_th %f\n", p->pr_pair_weights.compl_end_th) ; printf(" product_tm_lt %f\n", p->pr_pair_weights.product_tm_lt) ; printf(" product_tm_gt %f\n", p->pr_pair_weights.product_tm_gt) ; printf(" product_size_lt %f\n", p->pr_pair_weights.product_size_lt) ; printf(" product_size_gt %f\n", p->pr_pair_weights.product_size_gt) ; printf(" repeat_sim %f\n", p->pr_pair_weights.repeat_sim) ; printf(" template_mispriming %f\n", p->pr_pair_weights.template_mispriming) ; printf(" template_mispriming_th %f\n", p->pr_pair_weights.template_mispriming_th) ; printf(" end pair_weights\n") ; printf("\n\n"); printf("=============\n"); printf("BEGIN primer_args\n"); printf("begin oligo_weights\n"); printf("temp_gt %f\n", p->p_args.weights.temp_gt ) ; printf("temp_gt %f\n", p->p_args.weights.temp_gt) ; printf("temp_lt %f\n", p->p_args.weights.temp_lt) ; printf("gc_content_gt %f\n", p->p_args.weights.gc_content_gt) ; printf("gc_content_lt %f\n", p->p_args.weights.gc_content_lt) ; printf("compl_any %f\n", p->p_args.weights.compl_any) ; printf("compl_end %f\n", p->p_args.weights.compl_end) ; printf("compl_any_th %f\n", p->p_args.weights.compl_any_th) ; printf("compl_end_th %f\n", p->p_args.weights.compl_end_th) ; printf("hairpin %f\n", p->p_args.weights.hairpin_th) ; printf("repeat_sim %f\n", p->p_args.weights.repeat_sim) ; printf("length_lt %f\n", p->p_args.weights.length_lt) ; printf("length_gt %f\n", p->p_args.weights.length_gt) ; printf("seq_quality %f\n", p->p_args.weights.seq_quality) ; printf("end_quality %f\n", p->p_args.weights.end_quality) ; printf("pos_penalty %f\n", p->p_args.weights.pos_penalty) ; printf("end_stability %f\n", p->p_args.weights.end_stability) ; printf("num_ns %f\n", p->p_args.weights.num_ns) ; printf("template_mispriming %f\n", p->p_args.weights.template_mispriming) ; printf("template_mispriming_th %f\n", p->p_args.weights.template_mispriming_th) ; printf("end oligo_weights\n") ; printf("opt_tm %f\n", p->p_args.opt_tm) ; printf("min_tm %f\n", p->p_args.min_tm) ; printf("max_tm %f\n", p->p_args.max_tm) ; printf("opt_gc_content %f\n", p->p_args.opt_gc_content) ; printf("max_gc %f\n", p->p_args.max_gc) ; printf("min_gc %f\n", p->p_args.min_gc) ; printf("divalent_conc %f\n", p->p_args.divalent_conc) ; printf("dntp_conc %f\n", p->p_args.dntp_conc) ; printf("dna_conc %f\n", p->p_args.dna_conc) ; printf("num_ns_accepted %i\n", p->p_args.num_ns_accepted) ; printf("opt_size %i\n", p->p_args.opt_size) ; printf("min_size %i\n", p->p_args.min_size) ; printf("max_size %i\n", p->p_args.max_size) ; printf("max_poly_x %i\n", p->p_args.max_poly_x) ; printf("min_end_quality %i\n", p->p_args.min_end_quality) ; printf("min_quality %i\n", p->p_args.min_quality) ; printf("max_self_any %f\n", p->p_args.max_self_any) ; printf("max_self_end %f\n", p->p_args.max_self_end) ; printf("max_self_any_th %f\n", p->p_args.max_self_any_th) ; printf("max_self_end_th %f\n", p->p_args.max_self_end_th) ; printf("max_hairpin %f\n", p->p_args.max_hairpin_th) ; printf("max_repeat_compl %f\n", p->p_args.max_repeat_compl) ; printf("max_template_mispriming %f\n", p->p_args.max_template_mispriming) ; printf("max_template_mispriming_th %f\n", p->p_args.max_template_mispriming_th) ; printf("end primer args\n") ; printf("begin internal oligo args (p->o_args.)\n") ; printf(" begin internal oligo_weights (p->o_args.weights.)\n") ; printf(" temp_gt %f\n", p->o_args.weights.temp_gt) ; printf(" temp_lt %f\n", p->o_args.weights.temp_lt) ; printf(" gc_content_gt %f\n", p->o_args.weights.gc_content_gt) ; printf(" gc_content_lt %f\n", p->o_args.weights.gc_content_lt) ; printf(" compl_any %f\n", p->o_args.weights.compl_any) ; printf(" compl_end %f\n", p->o_args.weights.compl_end) ; printf(" compl_any_th %f\n", p->o_args.weights.compl_any_th) ; printf(" compl_end_th %f\n", p->o_args.weights.compl_end_th) ; printf(" hairpin %f\n", p->o_args.weights.hairpin_th) ; printf(" repeat_sim %f\n", p->o_args.weights.repeat_sim) ; printf(" length_lt %f\n", p->o_args.weights.length_lt) ; printf(" length_gt %f\n", p->o_args.weights.length_gt) ; printf(" seq_quality %f\n", p->o_args.weights.seq_quality) ; printf(" end_quality %f\n", p->o_args.weights.end_quality) ; printf(" pos_penalty %f\n", p->o_args.weights.pos_penalty) ; printf(" end_stability %f\n", p->o_args.weights.end_stability) ; printf(" num_ns %f\n", p->o_args.weights.num_ns) ; printf(" end internal oligo_weights\n") ; printf(" opt_tm %f\n", p->o_args.opt_tm) ; printf(" min_tm %f\n", p->o_args.min_tm) ; printf(" max_tm %f\n", p->o_args.max_tm) ; printf(" opt_gc_content %f\n", p->o_args.opt_gc_content) ; printf(" max_gc %f\n", p->o_args.max_gc) ; printf(" min_gc %f\n", p->o_args.min_gc) ; printf(" divalent_conc %f\n", p->o_args.divalent_conc) ; printf(" dntp_conc %f\n", p->o_args.dntp_conc) ; printf(" dna_conc %f\n", p->o_args.dna_conc) ; printf(" num_ns_accepted %i\n", p->o_args.num_ns_accepted) ; printf(" opt_size %i\n", p->o_args.opt_size) ; printf(" min_size %i\n", p->o_args.min_size) ; printf(" max_size %i\n", p->o_args.max_size) ; printf(" max_poly_x %i\n", p->o_args.max_poly_x) ; printf(" min_end_quality %i\n", p->o_args.min_end_quality) ; printf(" min_quality %i\n", p->o_args.min_quality) ; printf(" max_self_any %f\n", p->o_args.max_self_any) ; printf(" max_self_end %f\n", p->o_args.max_self_end) ; printf(" max_repeat_compl %f\n", p->o_args.max_repeat_compl) ; printf(" end internal oligo args\n"); printf("\n"); printf("END GLOBAL ARGS\n"); printf("=============\n"); printf("\n"); } if (s != NULL) { printf("=============\n"); printf("BEGIN SEQUENCE ARGS\n") ; /* TO DO: complete the statments for dumping this data printf("interval_array_t2 tar2 %i\n", int pairs[PR_MAX_INTERVAL_ARRAY][2]) ; int count) ; printf("interval_array_t2 excl2 %i\n", int pairs[PR_MAX_INTERVAL_ARRAY][2]) ; int count) ; printf("interval_array_t2 excl_internal2 %i\n", int pairs[PR_MAX_INTERVAL_ARRAY][2]) ; int count) ; printf("ok_regions \n"); */ if (s->primer_overlap_junctions_count > 0) { printf("primer_overlap_junctions_count %i\n", s->primer_overlap_junctions_count); printf("primer_overlap_junctions_list [\n"); for (i = 0; i < s->primer_overlap_junctions_count; i++) { printf(" %i\n", s->primer_overlap_junctions[i]); } printf("]\n"); } printf("incl_s %i\n", s->incl_s) ; printf("incl_l %i\n", s->incl_l) ; printf("start_codon_pos %i\n", s->start_codon_pos) ; printf("n_quality %i\n", s->n_quality) ; /* TO DO printf("quality%i\", s->quality) ; */ printf("quality_storage_size %i\n", s->quality_storage_size) ; printf("*sequence %s\n", s->sequence) ; printf("*sequence_name %s\n", s->sequence_name) ; printf("*sequence_file %s\n", s->sequence_file) ; printf("*trimmed_seq %s\n", s->trimmed_seq) ; printf("*trimmed_orig_seq %s\n", s->trimmed_orig_seq) ; printf("*upcased_seq %s\n", s->upcased_seq) ; printf("*upcased_seq_r %s\n", s->upcased_seq_r) ; printf("*left_input %s\n", s->left_input) ; printf("*right_input %s\n", s->right_input) ; printf("*internal_input %s\n", s->internal_input) ; printf("force_left_start %i\n", s->force_left_start) ; printf("force_left_end %i\n", s->force_left_end) ; printf("force_right_start %i\n", s->force_right_start) ; printf("force_right_end %i\n", s->force_right_end) ; printf("END SEQUENCE ARGS\n") ; printf("=============\n"); printf("\n"); } } primer3-2.3.6/src/Makefile0000664000077300007730000002166212231156245014314 0ustar ioanaioana# ====================================================================== # (c) Copyright 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009 # Whitehead Institute for Biomedical Research, Steve Rozen, # Andreas Untergasser and Helen Skaletsky # All rights reserved. # # This file is part of primer3, the libprimer3 library, the oligotm # library and the dpal library. # # Primer3 and the libraries above are free software; you can # redistribute them and/or modify them under the terms of the GNU # General Public License as published by the Free Software Foundation; # either version 2 of the License, or (at your option) any later # version. # # This software is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # # You should have received a copy of the GNU General Public License # along with this file (file gpl-2.0.txt in the source distribution); if # not, write to the Free Software Foundation, Inc., 51 Franklin St, # Fifth Floor, Boston, MA 02110-1301 USA # # THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS # "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT # LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR # A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT # OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, # SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT # LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, # DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY # THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT # (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE # OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. # ====================================================================== # ====================================================================== # CITING PRIMER3 # # Steve Rozen and Helen J. Skaletsky (2000) Primer3 on the WWW for # general users and for biologist programmers. In: Krawetz S, Misener S # (eds) Bioinformatics Methods and Protocols: Methods in Molecular # Biology. Humana Press, Totowa, NJ, pp 365-386. Source code available # from https://sourceforge.net/projects/primer3/ # ====================================================================== # For VALGRIND -- see end of this makefile for testing primer3_core # with valgrind, a leak and memory access checker. TESTOPTS= WINMAKE=mingw32-make LDLIBS = -lm AR = ar CC = gcc CPP = g++ O_OPTS = -O2 CC_OPTS = -g -Wall -D__USE_FIXED_PROTOTYPES__ P_DEFINES = CFLAGS = $(CC_OPTS) $(O_OPTS) LDFLAGS = -g # Note, for profiling, use # make O_OPTS='-pg -O0' LDFLAGS='-g -pg' # ====================================================================== # If you have trouble with library skew when moving primer3 executables # between systems, you might want to set LIBOPTS to -static LIBOPTS = ifeq ($(TESTOPTS),--windows) PRIMER_EXE = primer3_core.exe NTDPAL_EXE = ntdpal.exe NTTHAL_EXE = ntthal.exe OLIGOTM_EXE = oligotm.exe LONG_SEQ_EXE = long_seq_tm_test.exe else PRIMER_EXE = primer3_core NTDPAL_EXE = ntdpal NTTHAL_EXE = ntthal OLIGOTM_EXE = oligotm LONG_SEQ_EXE = long_seq_tm_test endif LIBOLIGOTM = liboligotm.a LIBOLIGOTM_DYN = liboligotm.so.1.2.0 LIBDPAL = libdpal.a LIBDPAL_DYN = libdpal.a.so.1.0.0 LIBTHAL = libthal.a LIBTHAL_DYN = libthal.a.so.1.0.0 LIBPRIMER3 = libprimer3.a LIBPRIMER3_DYN = libprimer3.so.1.0.0 LIBRARIES = $(LIBPRIMER3) $(LIBDPAL) $(LIBTHAL) $(LIBOLIGOTM) DYNLIBS = $(LIBPRIMER3_DYN) $(LIBDPAL_DYN) $(LIBTHAL_DYN) $(LIBOLIGOTM_DYN) RANLIB = ranlib PRIMER_OBJECTS1=primer3_boulder_main.o\ format_output.o\ read_boulder.o\ print_boulder.o PRIMER_OBJECTS=$(PRIMER_OBJECTS1) $(LIBRARIES) PRIMER_DYN_OBJECTS=$(PRIMER_OBJECTS1) $(DYNLIBS) # These are files generated by running ./primer3_core < ../example example_files=example.for example.rev example.int EXES=$(PRIMER_EXE) $(NTDPAL_EXE) $(NTTHAL_EXE) $(OLIGOTM_EXE) $(LONG_SEQ_EXE) all: $(EXES) $(LIBRARIES) clean_src: ifeq ($(TESTOPTS),--windows) del /Q /F *.o $(EXES) *~ $(LIBRARIES) $(example_files) core ..\*~ else -rm -f *.o $(EXES) *~ $(LIBRARIES) $(DYNLIBS) $(example_files) core ../*~ endif clean: clean_src ifeq ($(TESTOPTS),--windows) cd ..\test & $(WINMAKE) clean TESTOPTS=$(TESTOPTS) else cd ../test/; make clean endif $(LIBOLIGOTM): oligotm.o $(AR) rv $@ oligotm.o $(RANLIB) $@ $(LIBOLIGOTM_LIB): oligotm.o $(CC) -shared -W1,-soname,liboligotm.so.1 -o $(LIBOLIGOTM_DYN) oligotm.o $(LIBDPAL): dpal_primer.o $(AR) rv $@ dpal_primer.o $(RANLIB) $@ $(LIBDPAL_DYN): dpal_primer.o $(CC) -shared -W1,-soname,libdpal.so.1 -o $(LIBDPAL_DYN_LIB) dpal_primer.o $(LIBTHAL): thal_primer.o $(AR) rv $@ thal_primer.o $(RANLIB) $@ $(LIBTHAL_DYN): thal_primer.o $(CC) -shared -W1,-soname,libthal.so.1 -o $(LIBTHAL_DYN_LIB) thal_primer.o $(LIBPRIMER3): libprimer3.o p3_seq_lib.o $(AR) rv $@ libprimer3.o p3_seq_lib.o $(RANLIB) $@ $(LIBPRIMER3_DYN): libprimer3.o p3_seq_lib.o $(CC) -shared -W1,-soname,liprimer3.so.1 -o $(LIBPRIMER3_DYN) libprimer3.o p3_seq_lib.o $(PRIMER_EXE): $(PRIMER_OBJECTS) $(CPP) $(LDFLAGS) -o $@ $(PRIMER_OBJECTS) $(LIBOPTS) $(LDLIBS) libprimer3.o: libprimer3.c libprimer3.h p3_seq_lib.h dpal.h thal.h oligotm.h $(CPP) -c $(CFLAGS) -Wno-deprecated $(P_DEFINES) -o $@ libprimer3.c $(NTDPAL_EXE): ntdpal_main.o dpal.o $(CPP) $(LDFLAGS) -o $@ ntdpal_main.o dpal.o $(NTTHAL_EXE): thal_main.o thal.o $(CPP) $(LDFLAGS) -o $@ thal_main.o thal.o $(LDLIBS) $(OLIGOTM_EXE): oligotm_main.c oligotm.h $(LIBOLIGOTM) $(CPP) $(CFLAGS) -o $@ oligotm_main.c $(LIBOLIGOTM) $(LIBOPTS) $(LDLIBS) $(LONG_SEQ_EXE): long_seq_tm_test_main.c oligotm.o $(CPP) $(CFLAGS) -o $@ long_seq_tm_test_main.c oligotm.o $(LIBOPTS) $(LDLIBS) read_boulder.o: read_boulder.c read_boulder.h libprimer3.h dpal.h thal.h p3_seq_lib.h $(CPP) -c $(CFLAGS) $(P_DEFINES) -o $@ read_boulder.c print_boulder.o: print_boulder.c print_boulder.h libprimer3.h p3_seq_lib.h $(CPP) -c $(CFLAGS) $(P_DEFINES) -o $@ print_boulder.c dpal.o: dpal.c dpal.h $(CPP) -c $(CFLAGS) -o $@ dpal.c # We use '-ffloat-store' on windows to prevent undesirable # precision which may lead to differences in floating point results. thal.o: thal.c thal.h $(CPP) -c $(CFLAGS) -ffloat-store -o $@ thal.c p3_seq_lib.o: p3_seq_lib.c p3_seq_lib.h libprimer3.h $(CPP) -c $(CFLAGS) -o $@ p3_seq_lib.c dpal_primer.o: dpal.c dpal.h $(CPP) -c $(CFLAGS) $(P_DEFINES) -o $@ dpal.c thal_primer.o: thal.c thal.h $(CPP) -c $(CFLAGS) -ffloat-store $(P_DEFINES) -o $@ thal.c format_output.o: format_output.c format_output.h libprimer3.h dpal.h thal.h p3_seq_lib.h $(CPP) -c $(CFLAGS) $(P_DEFINES) -o $@ format_output.c ntdpal_main.o: ntdpal_main.c dpal.h $(CPP) -c $(CC_OPTS) -o $@ ntdpal_main.c thal_main.o: thal_main.c thal.h $(CPP) -c $(CFLAGS) -o $@ thal_main.c # We use CC_OPTS above rather than CFLAGS because # gcc 2.7.2 crashes while compiling ntdpal_main.c with -O2 oligotm.o: oligotm.c oligotm.h primer3_boulder_main.o: primer3_boulder_main.c libprimer3.h dpal.h thal.h oligotm.h format_output.h print_boulder.h read_boulder.h $(CPP) -c $(CFLAGS) $(P_DEFINES) primer3_boulder_main.c primer_test: test test: $(PRIMER_EXE) $(NTDPAL_EXE) $(NTTHAL_EXE) ifeq ($(TESTOPTS),--windows) cd ..\test & $(WINMAKE) TESTOPTS=$(TESTOPTS) else cd ../test; make test endif # ====================================================================== # # VALGRIND INSTRUCTIONS. # # These instructions work for linux, and run the 'memcheck' # functionality of valgrind. Tests will be _much_ slower # when running with valgrind checks. # # If necessary, get and install valgrind (should come with most # Linux's, but you need valgrind >= 3.2.3) # # In the src directory for primer3: # # $ make clean # # Re-complile without optimization and run the normal tests, does # _not_ valgrind them: # # $ make O_OPTS=-O0 test # The value of O_OPTS is Minus Oh Zero # # Run valgrind on a small example: # # $ valgrind --leak-check=yes --show-reachable=yes --log-file-exactly=p3vg ./primer3_core < ../example # # Check output in file p3vg # # Note: Valgrind is at /usr/local/bin/valgrind at WI # # OK, now for the real tests: # # $ cd ../test # # Check valgrind path in p3test.pl, ntdpal_test.pl, and oligotm_test.pl # and correct if necessary. This code works under valgrind 3.2.3. # # $ make TESTOPTS=--valgrind # Remember, you have to be in test/ # # When the --valgrind flag is set, the perl test scripts grep for and # display errors and leaks, but you need to ***look at the output** to # see these and figure out which test caused the problem, so you can # debug it. # # ====================================================================== primer3-2.3.6/src/thal_main.c0000775000077300007730000002431112231156245014751 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), and Helen Skaletsky All rights reserved. This file is part of primer3 software suite. This software suite is is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this software (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON A THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include #include #include #include #include #include #include #include #include #include #include #include "thal.h" /* Check on which OS we compile */ #if defined(_WIN32) || defined(WIN32) || defined (__WIN32__) || defined(__CYGWIN__) || defined(__MINGW32__) #define OS_WIN #endif #define DEBUG char *endptr; /* reading input */ int i; /* index */ const unsigned char *oligo1, *oligo2; /* inserted oligo sequences */ char *path = NULL; /* path to the parameter files */ /* Beginning of main */ int main(int argc, char** argv) { const char* usage; int tmp_ret; thal_args a; thal_results o; set_thal_default_args(&a); a.temponly=0; /* by default print only melting temperature, do not draw structure or print any additional parameters */ if(a.debug == 0) { #undef DEBUG } else { #define DEBUG } usage = "USAGE: %s OPTIONS oligo\n" "-mv monovalent_conc - concentration of monovalent cations in mM, by default 50 mM\n" "\n" "-dv divalent_conc - concentration of divalent cations in mM, by default 0 mM\n" "\n" "-n dNTP_conc - concentration of deoxynycleotide triphosphate in mM, by default 0 mM\n" "\n" "-d dna_conc - concentration of DNA strands in nM, by default 50 nM\n" "\n" "-a mode - alignment type, END1, END2, ANY and HAIRPIN, by default ANY (when duplex)\n" "\n" "-t temp - temperature at which duplex is calculated, by default 37C\n" "\n" "-r - causes the alignment NOT to be displayed on stderr, _only_ Tm is printed\n" "\n" "-maxloop size - the maximum size of secondary structures loops.\n" " Default is 30 (this is maximum allowed length, currently).\n" "\n" "-path - the path to the thermodynamic parameter files\n" "\n" "-s1 DNA_oligomer\n" "\n" "-s2 DNA_oligomer\n" "\n"; if(argc < 2) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif return -1; } /* BEGIN: READ the INPUT */ for(i = 1; i < argc; ++i) { if (!strncmp("-mv", argv[i], 3)) { /* conc of monovalent cations */ if(argv[i+1]==NULL) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } a.mv = strtod(argv[i+1], &endptr); if ('\0' != *endptr || a.mv < 0.0) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } i++; } else if (!strncmp("-dv", argv[i], 3)) { /* conc of divalent cations */ if(argv[i+1]==NULL) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } a.dv = strtod(argv[i+1], &endptr); if('\0' != *endptr || a.dv < 0.0) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } i++; } else if (!strcmp("-path", argv[i])) { if(argv[i+1]==NULL) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } path = (char*)argv[i+1]; i++; } else if (!strncmp("-s1", argv[i], 3)) { /* first sequence in 5'->3' direction */ if(argv[i+1]==NULL) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } oligo1 = (const unsigned char*)argv[i+1]; i++; } else if (!strncmp("-s2", argv[i], 3)) { /* second sequence in 5'->3' direction */ if(argv[i+1]==NULL) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } oligo2 = (const unsigned char*)argv[i+1]; i++; } else if (!strncmp("-a", argv[i], 2)) { /* annealing type END1, END2, ANY, considered only when duplexis; by default ANY */ if(argv[i+1]==NULL) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } if(strcmp(argv[i+1],"END1")==0) { a.type = thal_end1; } else if(strcmp(argv[i+1],"END2")==0) { a.type = thal_end2; } else if(strcmp(argv[i+1],"HAIRPIN")==0) { a.type = thal_hairpin; a.dimer = 0; } else if (strcmp(argv[i+1], "ANY")==0) { a.type = thal_any; /* ANY */ } else { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } i++; } else if (!strncmp("-d", argv[i], 2)) { /* dna conc */ if(argv[i+1]==NULL) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } a.dna_conc = strtod(argv[i+1], &endptr); if('\0' != *endptr || a.dna_conc < 0 || a.dna_conc == 0) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } i++; } else if (!strncmp("-r", argv[i], 2)) { /* only temp is calculated */ a.temponly = 1; } else if (!strncmp("-t", argv[i], 2)) { /* temperature at which sec str are calculated */ if(argv[i+1]==NULL) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } a.temp = strtod(argv[i+1], &endptr) + ABSOLUTE_ZERO; if('\0' != *endptr) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } i++; } else if (!strncmp("-n", argv[i], 2)) { /* concentration of dNTPs */ if(argv[i+1]==NULL) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } a.dntp = strtod(argv[i+1], &endptr); if('\0' != *endptr || a.dntp < 0.0) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } i++; } else if (!strncmp("-maxloop", argv[i], 8)) { /* maximum size of loop calculated; this value can not be larger than 30 */ if(argv[i+1]==NULL) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } a.maxLoop = (int) (strtod(argv[i+1], &endptr)); if(a.maxLoop > MAX_LOOP ) { a.maxLoop = MAX_LOOP; #ifdef DEBUG fputs("Warning: the maximum size of secondary structures loop is set to default (30)\n", stderr); #endif } else if(a.maxLoop < MIN_LOOP) { a.maxLoop = MIN_LOOP; #ifdef DEBUG fputs("Warning: the maximum size of secondary structures loop was set to minimum size of allowed loop length (0)\n", stderr); #endif } if('\0' != *endptr || a.maxLoop < 0) { #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } i++; } else if(!strncmp("-", argv[i], 1)) { /* Unknown option. */ #ifdef DEBUG fprintf(stderr, usage, argv[0]); #endif exit(-1); } else { break; } } /* END reading INPUT */ /* check the input correctness */ if(a.dimer!=0 && (oligo2==NULL || oligo1==NULL)) { /* if user wants to calculate structure of dimer then two sequences must be defined*/ fprintf(stderr, usage, argv[0]); exit(-1); } if(a.dimer==0 && (oligo2==NULL && oligo1==NULL)) { /* if user wants to calculate structure of monomer then only one sequence must be defined */ fprintf(stderr, usage, argv[0]); exit(-1); } /* read thermodynamic parameters */ if (path == NULL) { /* check for the default paths */ struct stat st; #ifdef OS_WIN if ((stat(".\\primer3_config", &st) == 0) && S_ISDIR(st.st_mode)) { tmp_ret = get_thermodynamic_values(".\\primer3_config\\", &o); } else { /* no default directory found, error */ fprintf(stderr, "Error: thermodynamic approach chosen, but path to thermodynamic parameters not specified\n"); exit(-1); } #else if ((stat("./primer3_config", &st) == 0) && S_ISDIR(st.st_mode)) { tmp_ret = get_thermodynamic_values("./primer3_config/", &o); } else if ((stat("/opt/primer3_config", &st) == 0) && S_ISDIR(st.st_mode)) { tmp_ret = get_thermodynamic_values("/opt/primer3_config/", &o); } else { /* no default directory found, error */ fprintf(stderr, "Error: thermodynamic approach chosen, but path to thermodynamic parameters not specified\n"); exit(-1); } #endif } else tmp_ret = get_thermodynamic_values(path, &o); if (tmp_ret) { fprintf(stderr, "%s\n", o.msg); exit(-1); } /* execute thermodynamical alignemnt */ if(a.dimer==0 && oligo1!=NULL){ thal(oligo1,oligo1,&a,&o); } else if(a.dimer==0 && oligo1==NULL && oligo2!=NULL) { thal(oligo2,oligo2,&a,&o); } else { thal(oligo1,oligo2,&a,&o); } /* encountered error during thermodynamical calc */ if (o.temp == THAL_ERROR_SCORE) { tmp_ret = fprintf(stderr, "Error: %s\n", o.msg); exit(-1); } if(a.temponly==1) printf("%f\n",o.temp); /* cleanup */ destroy_thal_structures(); return EXIT_SUCCESS; } primer3-2.3.6/src/Makefile.OSX0000664000077300007730000002255512231156245014766 0ustar ioanaioana# ====================================================================== # (c) Copyright 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009 # Whitehead Institute for Biomedical Research, Steve Rozen, # Andreas Untergasser and Helen Skaletsky # All rights reserved. # # This file is part of primer3, the libprimer3 library, the oligotm # library and the dpal library. # # Primer3 and the libraries above are free software; you can # redistribute them and/or modify them under the terms of the GNU # General Public License as published by the Free Software Foundation; # either version 2 of the License, or (at your option) any later # version. # # This software is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # # You should have received a copy of the GNU General Public License # along with this file (file gpl-2.0.txt in the source distribution); if # not, write to the Free Software Foundation, Inc., 51 Franklin St, # Fifth Floor, Boston, MA 02110-1301 USA # # THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS # "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT # LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR # A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT # OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, # SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT # LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, # DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY # THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT # (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE # OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. # ====================================================================== # ====================================================================== # CITING PRIMER3 # # Steve Rozen and Helen J. Skaletsky (2000) Primer3 on the WWW for # general users and for biologist programmers. In: Krawetz S, Misener S # (eds) Bioinformatics Methods and Protocols: Methods in Molecular # Biology. Humana Press, Totowa, NJ, pp 365-386. Source code available # from https://sourceforge.net/projects/primer3/ # ====================================================================== # For VALGRIND -- see end of this makefile for testing primer3_core # with valgrind, a leak and memory access checker. TESTOPTS= WINMAKE=mingw32-make DEVELOPER=`xcode-select -print-path` # this is clumsy, but deals with the moving compilers on OSX ifndef SDKROOT SDKROOT=$(DEVELOPER)/Platforms/MacOSX.platform/Developer/SDKs/MacOSX10.6.sdk endif ifneq (exists, $(shell [ -d $(SDKROOT) ] && echo exists)) SDKROOT=$(DEVELOPER)/SDKs/MacOSX10.6.sdk endif ifndef MACOSX_DEPLOYMENT_TARGET MACOSX_DEPLOYMENT_TARGET=10.6 endif ifndef ARCHS ARCHS=i386 x86_64 endif LDLIBS = -lm CC = gcc CPP = g++ LIBTOOL = libtool O_OPTS = -O2 CC_OPTS = -g -Wall -D__USE_FIXED_PROTOTYPES__ -isysroot $(SDKROOT) -mmacosx-version-min=$(MACOSX_DEPLOYMENT_TARGET) $(foreach ARCH,$(ARCHS),-arch $(ARCH)) P_DEFINES = CFLAGS = $(CC_OPTS) $(O_OPTS) LDFLAGS = -g $(foreach ARCH,$(ARCHS),-arch $(ARCH)) # ====================================================================== # If you have trouble with library skew when moving primer3 executables # between systems, you might want to set LIBOPTS to -static LIBOPTS = ifeq ($(TESTOPTS),--windows) PRIMER_EXE = primer3_core.exe NTDPAL_EXE = ntdpal.exe NTTHAL_EXE = ntthal.exe OLIGOTM_EXE = oligotm.exe LONG_SEQ_EXE = long_seq_tm_test.exe else PRIMER_EXE = primer3_core NTDPAL_EXE = ntdpal NTTHAL_EXE = ntthal OLIGOTM_EXE = oligotm LONG_SEQ_EXE = long_seq_tm_test endif LIBOLIGOTM = liboligotm.a LIBOLIGOTM_DYN = liboligotm.so.1.2.0 LIBDPAL = libdpal.a LIBDPAL_DYN = libdpal.a.so.1.0.0 LIBTHAL = libthal.a LIBTHAL_DYN = libthal.a.so.1.0.0 LIBPRIMER3 = libprimer3.a LIBPRIMER3_DYN = libprimer3.so.1.0.0 LIBRARIES = $(LIBPRIMER3) $(LIBDPAL) $(LIBTHAL) $(LIBOLIGOTM) DYNLIBS = $(LIBPRIMER3_DYN) $(LIBDPAL_DYN) $(LIBTHAL_DYN) $(LIBOLIGOTM_DYN) PRIMER_OBJECTS1=primer3_boulder_main.o\ format_output.o\ read_boulder.o\ print_boulder.o PRIMER_OBJECTS=$(PRIMER_OBJECTS1) $(LIBRARIES) PRIMER_DYN_OBJECTS=$(PRIMER_OBJECTS1) $(DYNLIBS) # These are files generated by running ./primer3_core < ../example example_files=example.for example.rev example.int EXES=$(PRIMER_EXE) $(NTDPAL_EXE) $(NTTHAL_EXE) $(OLIGOTM_EXE) $(LONG_SEQ_EXE) all: $(EXES) $(LIBRARIES) clean_src: ifeq ($(TESTOPTS),--windows) del /Q /F *.o $(EXES) *~ $(LIBRARIES) $(example_files) core ..\*~ else -rm -f *.o $(EXES) *~ $(LIBRARIES) $(DYNLIBS) $(example_files) core ../*~ endif clean: clean_src ifeq ($(TESTOPTS),--windows) cd ..\test & $(WINMAKE) clean TESTOPTS=$(TESTOPTS) else cd ../test/; make clean endif $(LIBOLIGOTM): oligotm.o $(LIBTOOL) -static -o $@ oligotm.o $(LIBOLIGOTM_LIB): oligotm.o $(CC) -shared -W1,-soname,liboligotm.so.1 -o $(LIBOLIGOTM_DYN) oligotm.o $(LIBDPAL): dpal_primer.o $(LIBTOOL) -static -o $@ dpal_primer.o $(LIBDPAL_DYN): dpal_primer.o $(CC) -shared -W1,-soname,libdpal.so.1 -o $(LIBDPAL_DYN_LIB) dpal_primer.o $(LIBTHAL): thal_primer.o $(LIBTOOL) -static -o $@ thal_primer.o $(LIBTHAL_DYN): thal_primer.o $(CC) -shared -W1,-soname,libthal.so.1 -o $(LIBTHAL_DYN_LIB) thal_primer.o $(LIBPRIMER3): libprimer3.o p3_seq_lib.o $(LIBTOOL) -static -o $@ libprimer3.o p3_seq_lib.o $(LIBPRIMER3_DYN): libprimer3.o p3_seq_lib.o $(CC) -shared -W1,-soname,liprimer3.so.1 -o $(LIBPRIMER3_DYN) libprimer3.o p3_seq_lib.o $(PRIMER_EXE): $(PRIMER_OBJECTS) $(CPP) $(LDFLAGS) -o $@ $(PRIMER_OBJECTS) $(LIBOPTS) $(LDLIBS) libprimer3.o: libprimer3.c libprimer3.h p3_seq_lib.h dpal.h thal.h oligotm.h $(CPP) -c $(CFLAGS) -Wno-deprecated $(P_DEFINES) -o $@ libprimer3.c $(NTDPAL_EXE): ntdpal_main.o dpal.o $(CPP) $(LDFLAGS) -o $@ ntdpal_main.o dpal.o $(NTTHAL_EXE): thal_main.o thal.o $(CPP) $(LDFLAGS) -o $@ thal_main.o thal.o $(LDLIBS) $(OLIGOTM_EXE): oligotm_main.c oligotm.h $(LIBOLIGOTM) $(CPP) $(CFLAGS) -o $@ oligotm_main.c $(LIBOLIGOTM) $(LIBOPTS) $(LDLIBS) $(LONG_SEQ_EXE): long_seq_tm_test_main.c oligotm.o $(CPP) $(CFLAGS) -o $@ long_seq_tm_test_main.c oligotm.o $(LIBOPTS) $(LDLIBS) read_boulder.o: read_boulder.c read_boulder.h libprimer3.h dpal.h thal.h p3_seq_lib.h $(CPP) -c $(CFLAGS) $(P_DEFINES) -o $@ read_boulder.c print_boulder.o: print_boulder.c print_boulder.h libprimer3.h p3_seq_lib.h $(CPP) -c $(CFLAGS) $(P_DEFINES) -o $@ print_boulder.c dpal.o: dpal.c dpal.h $(CPP) -c $(CFLAGS) -o $@ dpal.c # We use '-ffloat-store' on windows to prevent undesirable # precision which may lead to differences in floating point results. thal.o: thal.c thal.h $(CPP) -c $(CFLAGS) -ffloat-store -o $@ thal.c p3_seq_lib.o: p3_seq_lib.c p3_seq_lib.h libprimer3.h $(CPP) -c $(CFLAGS) -o $@ p3_seq_lib.c dpal_primer.o: dpal.c dpal.h $(CPP) -c $(CFLAGS) $(P_DEFINES) -o $@ dpal.c thal_primer.o: thal.c thal.h $(CPP) -c $(CFLAGS) -ffloat-store $(P_DEFINES) -o $@ thal.c format_output.o: format_output.c format_output.h libprimer3.h dpal.h thal.h p3_seq_lib.h $(CPP) -c $(CFLAGS) $(P_DEFINES) -o $@ format_output.c ntdpal_main.o: ntdpal_main.c dpal.h $(CPP) -c $(CC_OPTS) -o $@ ntdpal_main.c thal_main.o: thal_main.c thal.h $(CPP) -c $(CFLAGS) -o $@ thal_main.c # We use CC_OPTS above rather than CFLAGS because # gcc 2.7.2 crashes while compiling ntdpal_main.c with -O2 oligotm.o: oligotm.c oligotm.h primer3_boulder_main.o: primer3_boulder_main.c libprimer3.h dpal.h thal.h oligotm.h format_output.h print_boulder.h read_boulder.h $(CPP) -c $(CFLAGS) $(P_DEFINES) primer3_boulder_main.c primer_test: test test: $(PRIMER_EXE) $(NTDPAL_EXE) $(NTTHAL_EXE) ifeq ($(TESTOPTS),--windows) cd ..\test & $(WINMAKE) TESTOPTS=$(TESTOPTS) else cd ../test; make test endif # ====================================================================== # # VALGRIND INSTRUCTIONS. # # These instructions work for linux, and run the 'memcheck' # functionality of valgrind. Tests will be _much_ slower # when running with valgrind checks. # # If necessary, get and install valgrind (should come with most # Linux's, but you need valgrind >= 3.2.3) # # In the src directory for primer3: # # $ make clean # # Re-complile without optimization and run the normal tests, does # _not_ valgrind them: # # $ make O_OPTS=-O0 test # The value of O_OPTS is Minus Oh Zero # # Run valgrind on a small example: # # $ valgrind --leak-check=yes --show-reachable=yes --log-file-exactly=p3vg ./primer3_core < ../example # # Check output in file p3vg # # Note: Valgrind is at /usr/local/bin/valgrind at WI # # OK, now for the real tests: # # $ cd ../test # # Check valgrind path in p3test.pl, ntdpal_test.pl, and oligotm_test.pl # and correct if necessary. This code works under valgrind 3.2.3. # # $ make TESTOPTS=--valgrind # Remember, you have to be in test/ # # When the --valgrind flag is set, the perl test scripts grep for and # display errors and leaks, but you need to ***look at the output** to # see these and figure out which test caused the problem, so you can # debug it. # # ====================================================================== primer3-2.3.6/src/libprimer3.h0000664000077300007730000014752112231156245015100 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009, 2010,2011,2012 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of primer3 and the libprimer3 library. Primer3 and the libprimer3 library are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this file (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #ifndef LIBPRIMER3_H #define LIBPRIMER3_H #include #include /* FILE */ #include #include /* SHRT_MIN, ULONG_MAX */ #include /* DBL_MIN */ #include "oligotm.h" #ifdef __cplusplus extern "C" { #endif /* ALIGN_SCORE_UNDEF is used only libprimer3 and clients, not in dpal */ #define ALIGN_SCORE_UNDEF -DBL_MAX /* These next 5 are exposed for format_output.c -- probabaly should be reviewed. */ #define PR_INFINITE_POSITION_PENALTY -1.0 #define PR_DEFAULT_INSIDE_PENALTY PR_INFINITE_POSITION_PENALTY #define PR_DEFAULT_OUTSIDE_PENALTY 0.0 #define PR_DEFAULT_PRODUCT_MAX_TM 1000000.0 #define PR_DEFAULT_PRODUCT_MIN_TM -1000000.0 #define PR_NULL_FORCE_POSITION -1000000 /* Exposed in the read_boulder input routine.... */ #define PR_NULL_START_CODON_POS -1000000 #define PR_DEFAULT_START_CODON_POS -2000000 #define PR_START_CODON_POS_IS_NULL(SA) ((SA)->start_codon_pos <= PR_NULL_START_CODON_POS) #define _PR_DEFAULT_POSITION_PENALTIES(PA) \ (PR_DEFAULT_INSIDE_PENALTY == pa->inside_penalty \ && PR_DEFAULT_OUTSIDE_PENALTY == pa->outside_penalty) #define PR_ALIGN_SCORE_PRECISION 100.0 #define MACRO_STRING(X) #X /* pr_progam_name must be set in main(). */ #define PR_ASSERT(COND) \ if (!(COND)) { \ fprintf(stderr, "%s:%s:%d, assertion (%s) failed\n", \ pr_program_name, __FILE__, __LINE__, \ MACRO_STRING(COND)); \ abort(); \ } /* Enum to define tasks primer3 can do */ typedef enum task { pick_pcr_primers = 0, /* For backward compatibility, equivalent to generic plus pick_left_primer =1 plus pick_right_primer = 1 plus pick_internal_oligo = 0 */ pick_pcr_primers_and_hyb_probe = 1, pick_left_only = 2, pick_right_only = 3, pick_hyb_probe_only = 4, generic = 5, pick_cloning_primers = 6, pick_discriminative_primers = 7, pick_sequencing_primers = 8, pick_primer_list = 9, check_primers = 10, } task; /* Enum explaining if output are pairs */ typedef enum p3_output_type { primer_pairs = 0, primer_list = 1, } p3_output_type; /* pr_append_str is an append-only string ADT. */ typedef struct pr_append_str { int storage_size; char *data; } pr_append_str; /* * Arguments to the primer program as a whole. Values for these arguments are * retained _across_ different input records. (These are the so-called * "Global" arguments in the documentation.) */ typedef struct oligo_weights { double compl_any; double compl_any_th; double compl_end; double compl_end_th; double end_quality; double end_stability; double gc_content_gt; double gc_content_lt; double hairpin_th; double length_gt; double length_lt; double num_ns; double pos_penalty; double repeat_sim; double seq_quality; double temp_cutoff; double temp_gt; double temp_lt; double template_mispriming; double template_mispriming_th; } oligo_weights; typedef struct pair_weights { double primer_quality; double io_quality; double diff_tm; double compl_any; double compl_any_th; double compl_end; double compl_end_th; double temp_cutoff; double product_tm_lt; double product_tm_gt; double product_size_lt; double product_size_gt; double repeat_sim; double template_mispriming; double template_mispriming_th; } pair_weights; typedef struct sequencing_parameters { int lead; int spacing; int interval; int accuracy; } sequencing_parameters; #include "p3_seq_lib.h" typedef struct args_for_one_oligo_or_primer { seq_lib *repeat_lib; oligo_weights weights; double opt_tm; double min_tm; double max_tm; double opt_gc_content; double max_gc; double min_gc; /* Warning: also used for product Tm (TO DO: factor this out) */ double salt_conc; double divalent_conc; /* DIVALENT_CONC and DNTP_CONC are both needed for enabling use of divalent cations for calculation of melting temperature of short and long oligos. The formula for converting the divalent cations to monovalent cations is in the paper [Ahsen von N, Wittwer CT, Schutz E (2001) "Oligonucleotide Melting Temperatures under PCR Conditions: Nearest-Neighbor Corrections for Mg^2+, Deoxynucleotide Triphosphate, and Dimethyl Sulfoxide Concentrations with Comparision to Alternative Empirical Formulas", Clinical Chemistry 47:1956-61 http://www.clinchem.org/cgi/content/full/47/11/1956] The default is 0.0. (New in v. 1.1.0, added by Maido Remm and Triinu Koressaar.) */ double dntp_conc; double dna_conc; int num_ns_accepted; int opt_size; int min_size; int max_size; int max_poly_x; /* * Maximum length of mononucleotide sequence in an * oligo. */ int min_end_quality; int min_quality; /* Minimum quality permitted for oligo sequence.*/ double max_self_any; double max_self_end; double max_self_any_th; double max_self_end_th; double max_hairpin_th; double max_repeat_compl; /* * Acceptable complementarity with repeat * sequences. */ double max_template_mispriming; double max_template_mispriming_th; char *must_match_five_prime; char *must_match_three_prime; /* Primers and Oligos must match this 5 prime and 3 prime sequences. This allows to select a set of primer pair with an identical 3' end to avoid primer dimers. On the 5 prime end bases quenching flourochromes can be avoided. The sequence must be 5 nucletides long and can contain the following letters: N Any nucleotide A Adenine G Guanine C Cytosine T Thymine R Purine (A or G) Y Pyrimidine (C or T) W Weak (A or T) S Strong (G or C) M Amino (A or C) K Keto (G or T) B Not A (G or C or T) H Not G (A or C or T) D Not C (A or G or T) V Not T (A or G or C) */ } args_for_one_oligo_or_primer; /* Maxima needed for interface data structures. */ #define PR_MAX_INTERVAL_ARRAY 200 /* * Maximum number of input intervals * supported; used for targets, excluded * regions, product-size intervals, etc. */ typedef struct p3_global_settings { /* ================================================== */ /* Arguments that control behavior of choose_primers() */ task primer_task; /* 2 if left primer only, 3 if right primer * only, 4 if internal oligo only. */ int pick_left_primer; int pick_right_primer; int pick_internal_oligo; int file_flag; /* TO DO, See if this can be factored out. */ int first_base_index; /* * The index of the first base in the input * sequence. This parameter is ignored within * pr_choice; pr_choice's caller must assure that * all indexes are 0-based. However, this * parameter should used by output routines to * adjust base indexes. */ int liberal_base; /* * If non-0 then turn characters other than * [ATGCNatgcn] into N. */ int num_return; /* The number of best primer pairs to return. */ int pick_anyway; /* Pick even if input primer or oligos violate constraints. */ int lib_ambiguity_codes_consensus; /* If non-0, treat ambiguity codes in a mispriming/mishyb library as representing a consensus. So, for example, S would match C or G. N would match any nucleotide. It turns out that this _not_ what one normally wants, since many libraries contain strings of N, which then match every oligo (very bad). */ int quality_range_min; int quality_range_max; /* ================================================== */ /* Arguments for individual oligos and/or primers */ args_for_one_oligo_or_primer p_args; args_for_one_oligo_or_primer o_args; tm_method_type tm_santalucia; /* tm_santalucia added by T.Koressaar for updated table thermodynamics. Specifies details of melting temperature calculation. (New in v. 1.1.0, added by Maido Remm and Triinu Koressaar.) A value of 1 (recommended) directs primer3 to use the table of thermodynamic values and the method for melting temperature calculation suggested in the paper [SantaLucia JR (1998) "A unified view of polymer, dumbbell and oligonucleotide DNA nearest-neighbor thermodynamics", Proc Natl Acad Sci 95:1460-65 http://dx.doi.org/10.1073/pnas.95.4.1460]. A value of 0 directs primer3 to a backward compatible calculation (in other words, the only calculation availble in previous version of primer3). This backward compatible calculation uses the table of thermodynamic parameters in the paper [Breslauer KJ, Frank R, Bloecker H and Marky LA (1986) "Predicting DNA duplex stability from the base sequence" Proc Natl Acad Sci 83:4746-50 http://dx.doi.org/10.1073/pnas.83.11.3746], and the method in the paper [Rychlik W, Spencer WJ and Rhoads RE (1990) "Optimization of the annealing temperature for DNA amplification in vitro", Nucleic Acids Res 18:6409-12 http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2243783]. The default value is 0 only for backward compatibility. */ salt_correction_type salt_corrections; /* salt_corrections added by T.Koressaar for salt correction for Tm calculation. A value of 1 (recommended) directs primer3 to use the salt correction formula in the paper [SantaLucia JR (1998) "A unified view of polymer, dumbbell and oligonucleotide DNA nearest-neighbor thermodynamics", Proc Natl Acad Sci 95:1460-65 http://dx.doi.org/10.1073/pnas.95.4.1460] A value of 0 directs primer3 to use the the salt correction formula in the paper [Schildkraut, C, and Lifson, S (1965) "Dependence of the melting temperature of DNA on salt concentration", Biopolymers 3:195-208 (not available on-line)]. This was the formula used in previous version of primer3. A value of 2 directs primer3 to use the salt correction formula in the paper [Owczarzy R, You Y, Moreira BG, Manthey JA, Huang L, Behlke MA and Walder JA (2004) "Effects of sodium ions on DNA duplex oligomers: Improved predictions of melting temperatures", Biochemistry 43:3537-54 http://dx.doi.org/10.1021/bi034621r]. The default is 0 only for backward compatibility. */ /* ================================================== */ /* Start of arguments applicable to primers but not oligos */ double max_end_stability; /* The maximum value allowed for the delta * G of disruption for the 5 3' bases of * a primer. */ int max_end_gc; /* The maximum number of allowed G or C * for the 5 3' bases of a primer. */ int gc_clamp; /* Required number of GCs at 3' end. */ /* ================================================== */ /* Start of arguments related to primer and/or oligo location in the template. */ int lowercase_masking; /* lowercase_masking added by T.Koressaar. Enables design of primers from lowercase masked template. A value of 1 directs primer3 to reject primers overlapping lowercase a base exactly at the 3' end. This property relies on the assumption that masked features (e.g. repeats) can partly overlap primer, but they cannot overlap the 3'-end of the primer. In other words, lowercase bases at other positions in the primer are accepted, assuming that the masked features do not influence the primer performance if they do not overlap the 3'-end of primer. */ sequencing_parameters sequencing; /* Used to calculate the position of sequencing primers */ double outside_penalty; /* Multiply this value times the number of NTs * from the 3' end to the the (unique) target to * get the 'position penalty'. * Meaningless if there are multiple targets * or if the primer cannot be part of a pair * that spans the target. */ double inside_penalty; /* Multiply this value times the number of NT * positions by which the primer overlaps * the (unique) target to the 'position penalty'. * Meaningless if there are multiple targets * or if the primer cannot be part of a pair * that spans the target. */ /* ================================================== */ /* Arguments for primer pairs and products. */ /* Warning: Use p3_empty_gs_product_size_range and p3_add_to_gs_product_size_range to set these next three slots. */ int pr_min[PR_MAX_INTERVAL_ARRAY]; /* Minimum product sizes. */ int pr_max[PR_MAX_INTERVAL_ARRAY]; /* Maximum product sizes. */ int num_intervals; /* * Number of product size intervals * (i.e. number of elements in pr_min and * pr_max) */ int product_opt_size; double product_max_tm; double product_min_tm; double product_opt_tm; double pair_max_template_mispriming; double pair_max_template_mispriming_th; double pair_repeat_compl; double pair_compl_any; double pair_compl_any_th; double pair_compl_end; double pair_compl_end_th; int thermodynamic_oligo_alignment; /* Enables to use approach of thermodynamical alignment for dimer and hairpin calculations. 0 = Use alignment *not* based on thermodynamics - the only dimer calculation approach until primer3 2.2.0. No hairpins are calculated. 1 = Use alignment based on thermodynamics. Hairpins are calculated */ int thermodynamic_template_alignment; /* Enables to use approach of thermodynamical alignment for template mispriming calculations. 0 = Use alignment *not* based on thermodynamics. 1 = Use alignment based on thermodynamics. */ double max_diff_tm; /* Maximum allowed difference between temperature of primer and temperature of product. Cannot be calculated until product is known. */ pair_weights pr_pair_weights; int min_left_three_prime_distance; int min_right_three_prime_distance; /* Minimum number of base pairs between the 3' ends of any two left or any two right primers when returning num_return primer pairs. The objective is get 'truly different' primer pairs. Please see the user documentation (primer3_manual.htm) for PRIMER_{LEFT,RIGHT}_MIN_THREE_PRIME_DISTANCE. */ int min_5_prime_overlap_of_junction; /* The number of basepairs the primer has to overlap an overlap junction. */ int min_3_prime_overlap_of_junction; int dump; /* dump fields for global settings and seq args if dump == 1 */ } p3_global_settings; typedef enum oligo_type { OT_LEFT = 0, OT_RIGHT = 1, OT_INTL = 2 } oligo_type; /* This struct captures informatin about the similarity of an oligo (primer) to elements in a mispriming (repeat) library (which is read in from a fasta file). */ typedef struct rep_sim { char *name; /* Name of the sequence format with maximum similarity to the oligo. */ short min; /* * The minimum score in slot 'score' (below). * (Used when the objective function involves * minimization of mispriming possibilities.) */ short max; /* The index of the maximum score in slot 'score' (below). */ double *score; /* * Array of similarity (i.e. false-priming) scores, * one for each entry in the 'repeat_lib' slot * of the primargs struct. In libprimer3.c, * score is set to NULL to indicate that * the rep_sim structure is uninitialized. */ } rep_sim; #if (ULONG_MAX < 4294967295UL) CANNOT COMPILE FOR THIS SYSTEM (< 32 bits in an unsigned long it) #endif typedef struct oligo_problems { unsigned long int prob; } oligo_problems; typedef struct primer_rec { rep_sim repeat_sim; /* Information on the best repeat library (mispriming library) * match for this oligo (primer), plus additional scores. */ double temp; /* The oligo melting temperature calculated for the * primer. */ double gc_content; double position_penalty; /* * Penalty for distance from "ideal" position as specified * by inside_penalty and outside_penalty. */ double quality; /* Part of objective function due to this primer. */ double end_stability; /* Delta G of disription of 5 3' bases. */ int start; /* Position of the 5'-most base within the primer WITH RESPECT TO THE seq_args FIELD trimmed_seq. */ int seq_quality; /* Minimum quality score of bases included. */ int seq_end_quality; /* Minimum quality core of the 5 3' bases. */ double self_any; /* Self complementarity as local alignment * 100. */ double self_end; /* Self complementarity at 3' end * 100. */ double hairpin_th; /* hairpin, thermodynamical approach and calculated as any */ double template_mispriming; /* Max 3' complementarity to any ectopic site in template on the given template strand. */ double template_mispriming_r; /* Max 3' complementarity to any ectopic site in the template on the reverse complement of the given template strand. */ char length; /* Length of the oligo. */ char num_ns; /* Number of Ns in the oligo. */ char must_use; /* Non-0 if the oligo must be used even if it is illegal. */ char overlaps; /* Non-0 if the oligo overlaps some oligo used in one of the best pairs. */ oligo_problems problems; char overlaps_overlap_position; char template_mispriming_ok; /* Non-0 if the oligo was checked for this already and it is ok. */ } primer_rec; const char * p3_primer_rec_problems_to_string(const primer_rec *); int p3_ol_is_ok(const primer_rec *); /* * The structure for a pair of primers and for a pair of * primers plus an internal oligo. */ typedef struct primer_pair { double pair_quality; /* Penalty value of the primer pair */ double diff_tm; /* Absolute value of the difference between melting * temperatures for left and right primers. */ double product_tm; /* Estimated melting temperature of the product. */ double product_tm_oligo_tm_diff; /* Difference in Tm between the primer with lowest Tm the product Tm. */ double t_opt_a; double compl_any; /* * Local complementarity score between left and right * primers (* 100). */ double compl_end; /* * 3'-anchored global complementatory score between * * left and right primers (* 100). */ double template_mispriming; /* Maximum total mispriming score of both primers to ectopic sites in the template, on "same" strand (* 100). */ double repeat_sim; /* Maximum total similarity of both primers to the * sequence from given file in fasta format. */ primer_rec *left; /* Left primer. */ primer_rec *right; /* Right primer. */ primer_rec *intl; /* Internal oligo. */ char must_use; int product_size; /* Product size. */ int target; /* * 1 if there is a target between the right and left * primers. */ char *rep_name; } primer_pair; typedef int interval_array_t[PR_MAX_INTERVAL_ARRAY][2]; typedef struct interval_array_t2 { int pairs[PR_MAX_INTERVAL_ARRAY][2]; int count; } interval_array_t2; typedef struct interval_array_t4 { int left_pairs[PR_MAX_INTERVAL_ARRAY][2]; int right_pairs[PR_MAX_INTERVAL_ARRAY][2]; int any_left; /* set to 1 if the empty pair ",," is given for any left interval */ int any_right; /* set to 1 if the empty pair ",," is given for any right interval */ int any_pair; /* set to 1 if both intervals are given as empty */ int count; /* total number of pairs */ } interval_array_t4; int interval_array_t2_count(const interval_array_t2 *array); const int * interval_array_t2_get_pair(const interval_array_t2 *array, int i); typedef struct oligo_stats { int considered; /* Total number of tested oligos of given type */ int ns; /* Number of oligos rejected because of Ns */ int target; /* Overlapping targets. */ int excluded; /* Overlapping excluded regions. */ int gc; /* Unacceptable GC content. */ int gc_clamp; /* Don't have required number of GCs at 3' end. */ int gc_end_high; /* Too many G+Cs at the 3' end. */ int temp_min; /* Melting temperature below t_min. */ int temp_max; /* Melting temperature more than t_max. */ int size_min; /* Primer shorter than minimal size. */ int size_max; /* Primer longer than minimal size. */ int compl_any; /* Self-complementarity too high. */ int compl_end; /* Self-complementarity at 3' end too high. */ int hairpin_th; /* Hairpin structure too stable in thermodynamical approach */ int repeat_score; /* Complementarity with repeat sequence too high.*/ int poly_x; /* Long mononucleotide sequence inside. */ int seq_quality; /* Low quality of bases included. */ int stability; /* Stability of 5 3' bases too high. */ int no_orf; /* Would not amplify any of the specified ORF (valid for left primers only). */ int template_mispriming; /* Template mispriming score too high. */ int ok; /* Number of acceptable oligos. */ int gmasked; /* Added by T. Koressaar, number of gmasked oligos */ int must_match_fail; /* Added by A. Untergasser, number of oligos failing must match */ int not_in_any_left_ok_region; /* Oligo not included in any of the left regions given in PRIMER_PAIR_OK_REGION_LIST. */ int not_in_any_right_ok_region;/* Oligo not included in any of the right regions given in PRIMER_PAIR_OK_REGION_LIST. */ } oligo_stats; typedef struct pair_stats { int considered; /* Total number of pairs or triples tested. */ int product; /* Pairs providing incorrect product size. */ int target; /* Pairs without any target between primers. */ int temp_diff; /* Melting temperature difference too high. */ int compl_any; /* Pairwise complementarity larger than allowed. */ int compl_end; /* The same for 3' end complementarity. */ int internal; /* Internal oligo was not found. */ int repeat_sim; /* Complementarity with repeat sequence too high.*/ int high_tm; /* Product Tm too high. */ int low_tm; /* Product Tm too low. */ int template_mispriming; /* Sum of template mispriming scores too high. */ /* Neither oligo in the pairs overlaps one of the "required sites". */ int does_not_overlap_a_required_point; /* One of the oligos in the pair overlaps an oligo in a better_pair: */ int overlaps_oligo_in_better_pair; /* The left and right oligos are not in any of the pair of regions given in PRIMER_PAIR_OK_REGION_LIST. */ int not_in_any_ok_region; /* Left primer to the right of right right primer. This can occur when the primers are provided by the caller. */ int reversed; int ok; /* Number that were ok. */ } pair_stats; typedef struct pair_array_t { int storage_size; int num_pairs; primer_pair *pairs; pair_stats expl; } pair_array_t; /* * Arguments relating to a single particular source sequence (for which * we will pick primer(s), etc. */ typedef struct seq_args { /* The net next 3 slots are presented as * indexes within the sequence slot, but * they are recalculated to be indexes * within trimmed_seq (i.e. within the * "included region"). */ interval_array_t2 tar2; /* The targets. tar2->pairs[i][0] is the start * of the ith target, tar2->pairs[i][1] its length. */ interval_array_t2 excl2;/* The number of excluded regions. */ interval_array_t2 excl_internal2; /* Number of excluded regions for internal oligo; similar to excl2.*/ interval_array_t4 ok_regions; int primer_overlap_junctions[PR_MAX_INTERVAL_ARRAY]; /* List of overlap junction positions. */ int primer_overlap_junctions_count; int incl_s; /* The 0-based start of included region. */ int incl_l; /* * The length of the included region, which is * also the length of the trimmed_seq field. */ int start_codon_pos; /* Index of first base of the start codon. */ int *quality; /* Vector of quality scores. */ int n_quality; /* Number of valid elements in 'quality' */ int quality_storage_size; /* Amount of storage quality points to. */ char *sequence; /* The template sequence itself as input, not trimmed, not up-cased. */ char *sequence_name; /* An identifier for the sequence. */ char *sequence_file; /* Another identifer for the sequence. */ char *trimmed_seq; /* The included region only, _UPCASED_. */ /* Element add by T. Koressaar support lowercase masking: */ char *trimmed_orig_seq; /* Trimmed version of the original, mixed-case sequence. */ char *upcased_seq; /* Upper case version of sequence (_not_ trimmed). */ char *upcased_seq_r; /* Upper case version of sequence, other strand (_not_ trimmed). */ char *left_input; /* A left primer to check or design around. */ char *right_input; /* A right primer to check or design around. */ char *internal_input; /* An internal oligo to check or design around. */ int force_left_start; /* The 0-based forced 5' start left primer. */ int force_left_end; /* The 0-based forced 3' end left primer. */ int force_right_start; /* The 0-based forced 5' start right primer. */ int force_right_end; /* The 0-based forced 3' end right primer. */ } seq_args; /* oligo_array is used to store a list of oligos or primers */ typedef struct oligo_array { /* Array of oligo (primer) records. */ primer_rec *oligo; /* Number of initialized elements */ int num_elem; /* Storage lengths of oligo */ int storage_size; /* Type of oligos in the array */ oligo_type type; /* Primers statistics. */ oligo_stats expl; } oligo_array; /* * The return value for for primer3. * After use, free memory with destroy_p3retval(). */ typedef struct p3retval { /* Arrays of oligo (primer) records. */ oligo_array fwd, intl, rev; /* Array of best primer pairs */ pair_array_t best_pairs; /* Enum to store type of output */ p3_output_type output_type; /* Place for error messages */ pr_append_str glob_err; pr_append_str per_sequence_err; pr_append_str warnings; /* * An optional _output_, meaninful if a * start_codon_pos is "not null". The position of * the intial base of the leftmost stop codon that * is to the right of sa->start_codon_pos. */ int stop_codon_pos; int upstream_stop_codon; /* TO DO needs docs */ } p3retval; /* Deallocate a primer3 state */ void destroy_p3retval(p3retval *); void destroy_dpal_thal_arg_holder(); /* get elements of p3retval */ const pair_array_t *p3_get_rv_best_pairs(const p3retval *r); const oligo_array *p3_get_rv_fwd(const p3retval *r); const oligo_array *p3_get_rv_intl(const p3retval *r); const oligo_array *p3_get_rv_rev(const p3retval *r); /* TO DO -- needs more documentation It is the responsibility of caller to free the return value. */ char *p3_get_rv_and_gs_warnings(const p3retval *retval, const p3_global_settings *pa); /* Return a char * describing global errors (usually/always?) errors caused by problems detected in the p3_global_settings * argument to choose primers. Returned storage is free'ed on calling destroy_p3retval(r). Returns NULL if no error. */ const char *p3_get_rv_global_errors(const p3retval *r); /* Return a char * describing per-sequence errors (usually/always?) errors caused by problems detected in the seq_args * argument to choose primers. Returned storage is free'ed on calling destroy_p3retval(r). Returns NULL if no error. */ const char *p3_get_rv_per_sequence_errors(const p3retval *r); /* Return a char * describing warnings generated in choose_primers. Returned storage is free'ed on calling destroy_p3retval(r). Returns NULL if no warnings. */ const char *p3_get_rv_warnings(const p3retval *r); p3_output_type p3_get_rv_output_type(const p3retval *r); int p3_get_rv_stop_codon_pos(p3retval *r); /* Get elements of an oligo_array */ int p3_get_oa_n(const oligo_array *x); const primer_rec *p3_get_oa_i(const oligo_array *x, int i); /* We still need accessors (getters) for the elements of primer_rec */ /* Functions for seq_args -- create, destroy, set, set slots */ seq_args *create_seq_arg(); void destroy_seq_args(seq_args *); int p3_set_sa_sequence(seq_args *sargs, const char *sequence); void p3_set_sa_primer_sequence_quality(seq_args *sargs, int quality); int p3_set_sa_sequence_name(seq_args *sargs, const char* sequence_name); int p3_set_sa_left_input(seq_args *sargs, const char *left_input); int p3_set_sa_right_input(seq_args *sargs, const char *right_input); int p3_set_sa_internal_input(seq_args *sargs, const char *internal_input); void p3_set_sa_empty_quality(seq_args *sargs); void p3_sa_add_to_quality_array(seq_args *sargs, int quality); int p3_sa_add_to_overlap_junctions_array(seq_args *, int); /* The following three functions return 0 on success, 1 on error (no space for additional intervals). */ int p3_add_to_sa_tar2(seq_args *, int, int); int p3_add_to_sa_excl2(seq_args *, int, int); int p3_add_to_sa_excl_internal2(seq_args *, int, int); int p3_add_to_sa_ok_regions(seq_args *, int, int, int, int); const interval_array_t2 *p3_get_sa_tar2(const seq_args *sargs); const interval_array_t2 *p3_get_sa_excl2(const seq_args *sargs); const interval_array_t2 *p3_get_sa_excl_internal2(const seq_args *sargs); const interval_array_t4 *p3_get_sa_ok_regions(const seq_args *sargs); const int* p3_get_sa_overlap_junctions(const seq_args *sargs); /* use p3_add_to_interval_array(interval_array_t2 *interval_arr, int i1, int i2); to do the sets for tar2, excl2, and excl_internal2 */ int p3_add_to_interval_array(interval_array_t2 *interval_arr, int i1, int i2); /* use p3_add_to_2_interval_array to do the sets for ok_regions */ int p3_add_to_2_interval_array(interval_array_t4 *interval_arr, int i1, int i2, int i3, int i4); /* included region */ void p3_set_sa_incl_s(seq_args *sargs, int incl_s); void p3_set_sa_incl_l(seq_args *sargs, int incl_l); void p3_set_sa_n_quality(seq_args *sargs, int n_quality) ; void p3_set_sa_start_codon_pos(seq_args *sargs, int start_codon_pos); int p3_set_sa_sequence_file(seq_args *sargs, const char *sequence_file); int p3_set_sa_trimmed_sequence(seq_args *sargs, const char *trimmed_sequence); int p3_set_sa_trimmed_original_sequence(seq_args *sargs, const char *trimmed_original_sequence); int p3_set_sa_upcased_sequence(seq_args *sargs, const char *upcased_sequencd); /* ============================================================ */ /* Functions for p3_global_settings -- create, destroy, set slots, get slots */ /* ============================================================ */ /* Use this for -default_version=2 (up-to-date defaults) */ p3_global_settings *p3_create_global_settings(); /* Use this for -default_version=1 (old defaults) */ p3_global_settings *p3_create_global_settings_default_version_1(); void p3_destroy_global_settings(p3_global_settings *); void p3_set_gs_prmin (p3_global_settings * p , int val, int i); void p3_set_gs_prmax (p3_global_settings * p , int val, int i); void p3_set_gs_primer_opt_size(p3_global_settings * p , int val); void p3_set_gs_primer_min_size(p3_global_settings * p , int val); void p3_set_gs_primer_max_size(p3_global_settings * p , int val); void p3_set_gs_primer_max_poly_x(p3_global_settings * p , int val); void p3_set_gs_primer_opt_tm(p3_global_settings * p , double product_opt_tm); void p3_set_gs_primer_opt_gc_percent(p3_global_settings * p , double val); void p3_set_gs_primer_min_tm(p3_global_settings * p , double product_min_tm); void p3_set_gs_primer_max_tm(p3_global_settings * p , double product_max_tm); void p3_set_gs_primer_max_diff_tm(p3_global_settings * p , double val); void p3_set_gs_primer_tm_santalucia(p3_global_settings * p, tm_method_type val); void p3_set_gs_primer_salt_corrections(p3_global_settings * p, salt_correction_type salt_corrections); void p3_set_gs_primer_min_gc(p3_global_settings * p , double val); void p3_set_gs_primer_max_gc(p3_global_settings * p , double val); void p3_set_gs_primer_salt_conc(p3_global_settings * p , double val); void p3_set_gs_primer_divalent_conc(p3_global_settings * p , double val); void p3_set_gs_primer_dntp_conc(p3_global_settings * p , double val); void p3_set_gs_primer_dna_conc(p3_global_settings * p , double val); void p3_set_gs_primer_num_ns_accepted(p3_global_settings * p , int val); void p3_set_gs_primer_product_opt_size(p3_global_settings * p , int val); void p3_set_gs_primer_self_any(p3_global_settings * p , double val); void p3_set_gs_primer_self_any_th(p3_global_settings * p , double val); void p3_set_gs_primer_self_end(p3_global_settings * p , double val); void p3_set_gs_primer_self_end_th(p3_global_settings * p , double val); void p3_set_gs_primer_hairpin_th(p3_global_settings * p , double val); void p3_set_gs_primer_file_flag(p3_global_settings * p , int val); void p3_set_gs_primer_pick_anyway(p3_global_settings * p , int val); void p3_set_gs_primer_gc_clamp(p3_global_settings * p , int val); void p3_set_gs_primer_explain_flag(p3_global_settings * p , int val); void p3_set_gs_primer_liberal_base(p3_global_settings * p , int val); void p3_set_gs_primer_first_base_index(p3_global_settings * p , int val); void p3_set_gs_primer_num_return(p3_global_settings * p , int val); void p3_set_gs_primer_min_quality(p3_global_settings * p , int val); void p3_set_gs_primer_min_end_quality(p3_global_settings * p , int val); void p3_set_gs_primer_quality_range_min(p3_global_settings * p , int val); void p3_set_gs_primer_quality_range_max(p3_global_settings * p , int val); void p3_set_gs_primer_product_max_tm(p3_global_settings * p , double val); void p3_set_gs_primer_product_min_tm(p3_global_settings * p , double val); void p3_set_gs_primer_product_opt_tm(p3_global_settings * p , double val); void p3_set_gs_primer_task(p3_global_settings * p , char * primer_task); void p3_set_gs_primer_pick_left_primer(p3_global_settings * p , int pick_left_primer); void p3_set_gs_primer_pick_right_primer(p3_global_settings * p , int pick_right_primer); void p3_set_gs_primer_pick_internal_oligo(p3_global_settings * p , int pick_internal_oligo); void p3_set_gs_primer_internal_oligo_opt_size(p3_global_settings * p , int val); void p3_set_gs_primer_internal_oligo_max_size(p3_global_settings * p , int val); void p3_set_gs_primer_internal_oligo_min_size(p3_global_settings * p , int val); void p3_set_gs_primer_internal_oligo_max_poly_x(p3_global_settings * p , int val); void p3_set_gs_primer_internal_oligo_opt_tm(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_max_tm(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_min_tm(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_min_gc(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_max_gc(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_salt_conc(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_divalent_conc(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_dntp_conc(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_dna_conc(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_num_ns(p3_global_settings * p , int val); void p3_set_gs_primer_internal_oligo_min_quality(p3_global_settings * p , int val); void p3_set_gs_primer_internal_oligo_self_any(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_self_any_th(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_self_end(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_self_end_th(p3_global_settings * p , double val); void p3_set_gs_primer_max_mispriming(p3_global_settings * p , double val); void p3_set_gs_primer_max_mispriming_th(p3_global_settings * p , double val); void p3_set_gs_primer_internal_oligo_max_mishyb(p3_global_settings * p , double val); void p3_set_gs_primer_pair_max_mispriming(p3_global_settings * p , double val); void p3_set_gs_primer_pair_max_mispriming_th(p3_global_settings * p , double val); void p3_set_gs_primer_max_template_mispriming(p3_global_settings * p , double val); void p3_set_gs_primer_max_template_mispriming_th(p3_global_settings * p , double val); void p3_set_gs_primer_lib_ambiguity_codes_consensus(p3_global_settings * p , int val); void p3_set_gs_primer_inside_penalty(p3_global_settings * p , double val); void p3_set_gs_primer_outside_penalty(p3_global_settings * p , double val); void p3_set_gs_primer_mispriming_library(p3_global_settings * p , char * val); void p3_set_gs_primer_internal_oligo_mishyb_library(p3_global_settings * p , char * val); void p3_set_gs_primer_max_end_stability(p3_global_settings * p , double val); void p3_set_gs_primer_lowercase_masking(p3_global_settings * p , int val); void p3_set_gs_primer_thermodynamic_oligo_alignment(p3_global_settings * p , int val); void p3_set_gs_primer_thermodynamic_template_alignment(p3_global_settings * p , int val); void p3_set_gs_primer_wt_tm_gt(p3_global_settings * p , double val); void p3_set_gs_primer_wt_tm_lt(p3_global_settings * p , double val); void p3_set_gs_primer_wt_gc_percent_gt(p3_global_settings * p , double val); void p3_set_gs_primer_wt_gc_percent_lt(p3_global_settings * p , double val); void p3_set_gs_primer_wt_size_lt(p3_global_settings * p , double val); void p3_set_gs_primer_wt_size_gt(p3_global_settings * p , double val); void p3_set_gs_primer_wt_compl_any(p3_global_settings * p , double val); void p3_set_gs_primer_wt_compl_any_th(p3_global_settings * p , double val); void p3_set_gs_primer_wt_compl_end(p3_global_settings * p , double val); void p3_set_gs_primer_wt_compl_end_th(p3_global_settings * p , double val); void p3_set_gs_primer_wt_hairpin_th(p3_global_settings * p , double val); void p3_set_gs_primer_wt_num_ns(p3_global_settings * p , double val); void p3_set_gs_primer_wt_rep_sim(p3_global_settings * p , double val); void p3_set_gs_primer_wt_seq_qual(p3_global_settings * p , double val); void p3_set_gs_primer_wt_end_qual(p3_global_settings * p , double val); void p3_set_gs_primer_wt_pos_penalty(p3_global_settings * p , double val); void p3_set_gs_primer_wt_end_stability(p3_global_settings * p , double val); void p3_set_gs_primer_wt_template_mispriming(p3_global_settings * p , double val); void p3_set_gs_primer_wt_template_mispriming_th(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_tm_gt(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_tm_lt(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_gc_percent_gt(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_gc_percent_lt(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_size_lt(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_size_gt(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_wt_coml_any(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_compl_end(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_wt_coml_any_th(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_compl_end_th(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_hairpin_th(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_num_ns(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_rep_sim(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_seq_qual(p3_global_settings * p , double val); void p3_set_gs_primer_io_wt_end_qual(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_pr_penalty(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_io_penalty(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_diff_tm(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_compl_any(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_compl_any_th(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_compl_end(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_compl_end_th(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_hairpin_th(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_product_tm_lt(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_product_tm_gt(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_product_size_gt(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_product_size_lt(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_rep_sim(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_template_mispriming(p3_global_settings * p , double val); void p3_set_gs_primer_pair_wt_template_mispriming_th(p3_global_settings * p , double val); void p3_set_gs_first_base_index(p3_global_settings * p , int first_base_index); void p3_set_gs_liberal_base(p3_global_settings * p , int liberal_base); void p3_set_gs_num_return(p3_global_settings * p , int num_return); void p3_set_gs_pick_anyway(p3_global_settings * p , int pick_anyway); void p3_set_gs_lib_ambiguity_codes_consensus(p3_global_settings * p , int lib_ambiguity_codes_consensus); void p3_set_gs_quality_range_min(p3_global_settings * p , int quality_range_min); void p3_set_gs_quality_range_max(p3_global_settings * p , int quality_range_max); void p3_empty_gs_product_size_range(p3_global_settings *pgs); /* Return 1 on error (product size range is full); otherwise return 0. */ int p3_add_to_gs_product_size_range(p3_global_settings *, int, int); args_for_one_oligo_or_primer *p3_get_global_settings_p_args(p3_global_settings * p); args_for_one_oligo_or_primer *p3_get_global_settings_o_args(p3_global_settings * p); int p3_set_afogop_seq_lib(args_for_one_oligo_or_primer *, seq_lib *); int p3_set_afogop_opt_tm(args_for_one_oligo_or_primer *, double); /* max_end_gc must be >= 0 and <= 5 */ void p3_set_gs_max_end_gc(p3_global_settings *p, int max_end_gc); void p3_set_gs_max_end_stability(p3_global_settings * p , int max_end_stability); void p3_set_gs_gc_clamp(p3_global_settings * p , int gc_clamp); void p3_set_gs_lowercase_masking(p3_global_settings * p , int lowercase_masking); void p3_set_gs_outside_penalty(p3_global_settings * p , double outside_penalty); void p3_set_gs_inside_penalty(p3_global_settings * p , double inside_penalty); /* DO NOT USE */ /* void p3_set_gs_num_intervals(p3_global_settings * p , int num_intervals); */ void p3_set_gs_pair_max_template_mispriming(p3_global_settings * p , double pair_max_template_mispriming); void p3_set_gs_pair_max_template_mispriming_th(p3_global_settings * p , double pair_max_template_mispriming_th); void p3_set_gs_pair_repeat_compl(p3_global_settings * p, double pair_repeat_compl); void p3_set_gs_pair_compl_any(p3_global_settings * p , double pair_compl_any); void p3_set_gs_pair_compl_end(p3_global_settings * p , double pair_compl_end); void p3_set_gs_pair_compl_any_th(p3_global_settings * p , double pair_compl_any_th); void p3_set_gs_pair_compl_end_th(p3_global_settings * p , double pair_compl_end_th); void p3_set_gs_min_left_three_prime_distance(p3_global_settings *p, int min_distance); void p3_set_gs_min_right_three_prime_distance(p3_global_settings *p, int min_distance); void p3_set_gs_min_5_prime_overlap_of_junction(p3_global_settings *p, int min_5_prime); void p3_set_gs_min_3_prime_overlap_of_junction(p3_global_settings *p, int min_3_prime); /* * Choose individual primers or oligos, or primer pairs, or primer * pairs with internal oligos. On ENOMEM return NULL and set errno. * Otherwise return retval (updated). Errors are returned in * in retval. */ p3retval *choose_primers(const p3_global_settings *pa, seq_args *sa); /* For testing/debugging: print the values in pa and sa to stdout. */ void p3_print_args(const p3_global_settings *pa, seq_args *sa) ; /* Print out the content of one primer array */ /* Return 1 on error, otherwise 0. */ int p3_print_one_oligo_list(const seq_args *, int, const primer_rec[], const oligo_type, const int, const int, FILE *,int); char *pr_oligo_sequence(const seq_args *, const primer_rec *); char *pr_oligo_rev_c_sequence(const seq_args *, const primer_rec *); /* Return NULL on ENOMEM */ pr_append_str *create_pr_append_str(); void init_pr_append_str(pr_append_str *s); void pr_set_empty(pr_append_str *); int pr_is_empty(const pr_append_str *); void destroy_pr_append_str(pr_append_str *); void destroy_pr_append_str_data(pr_append_str *str); /* Return 1 on ENOMEM, otherwise 0 */ int pr_append_external(pr_append_str *, const char *); int pr_append_w_sep_external(pr_append_str *x, const char *sep, const char *s); int pr_append_new_chunk_external(pr_append_str *, const char *); const char * pr_append_str_chars(const pr_append_str *x); /* Warning, return pointers to static storage; overwritten on each call. */ const char *p3_get_pair_array_explain_string(const pair_array_t *); const char *p3_get_oligo_array_explain_string(const oligo_array *); const char *libprimer3_release(void); const char *primer3_copyright(void); /* An accessor function for a primer_rec *. */ double oligo_max_template_mispriming(const primer_rec *); double oligo_max_template_mispriming_thermod(const primer_rec *); int strcmp_nocase(const char *, const char *); void p3_set_program_name(const char *name); /* * Utility function for C clients -- will not overflow * buffer. Warning: points to static storage that is over-written * on the next call. */ char* p3_read_line(FILE *file); /* Return 1 iff the argument 'oligo' has any problems -- i.e. violations of design constraints. */ int p3_ol_has_any_problem(const primer_rec *oligo); /* Return a string details the the problems in 'oligo', i.e. the constraints that 'oligo' violates. WARNING: Returns a pointer to static storage, which is over-written on next call. */ const char *p3_get_ol_problem_string(const primer_rec *oligo); /* Creates up to three files, the names of which are based on the argument 'file'. One file is a table of forward primers, one a table of reverse primers, and one a table of internal hybridization oligos, depending on what the caller to choose_primers() requested (in the 'pa' argument). Returns 0 on success, 1 on error. On error, check errno for ENOMEM. Used to implement P3_FILE_FLAG=1. */ int p3_print_oligo_lists(const p3retval*, const seq_args *, const p3_global_settings *, pr_append_str *err, const char* file_name); /* Translate the values in the stats struct into an warning string. Call this function only if the 'stat's contains the _errors_ associated with a given primer i.e. that primer was supplied by the caller and pick_anyway is set. */ void add_must_use_warnings(pr_append_str *warning, const char* text, const oligo_stats *stats); /* Reverse and complement the sequence seq and put the result in s. WARNING: It is up the caller to ensure that s points to enough space. */ void p3_reverse_complement(const char *, char *); #ifdef __cplusplus } #endif #endif primer3-2.3.6/src/long_seq_tm_test_main.c0000664000077300007730000000575312231156245017375 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky. All rights reserved. This file is part of the primer3 suite and libraries. The primer3 suite and libraries are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this software (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ /* Test main for function long_seq_tm() in oligotm.c Usage is e.g long_seq_tm_test AAAAGGGCCCCCCCCTTTTTTTTTTT 3 20 In this example 3 is the 0-based start of the substring to use and 30 is the length of the substring to use, so Tm is calculated on GGGCCCCCCCCTTTTTTTTT (= 52.452902). For testing compare to independent implementation in ../test/long_seq_tm_test.pl. */ #include #include #include #include #include "oligotm.h" /* double long_seq_tm(const char* s, int start, int len, double salt_conc, double divalent_conc, double dntp_conc); */ int main(int argc, const char**argv) { const char *s; double salt_conc = 50; double divalent_conc = 0; double dntp_conc = 0; double tm; int start, len; char *endptr; s = argv[1]; if (0 == s) { fprintf(stderr, "\n%s: incorrect arguments.\n", argv[0]); fprintf(stderr, "See file long_seq_tm_test_main.c for usage.\n\n"); exit(-1); } start = strtol(argv[2], &endptr, 10); len = strtol(argv[3], &endptr, 10); printf("s=%s, start=%d, length=%d\n", s, start, len); tm = long_seq_tm(s, start, len, salt_conc, divalent_conc, dntp_conc); printf("tm = %f\n", tm); return 0; } primer3-2.3.6/src/p3_seq_lib.h0000664000077300007730000000703112231156245015037 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of primer3 and primer3 software suite. Primer3 and the primer3 software suite are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this file (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #ifndef P3_SEQ_LIB_H #define P3_SEQ_LIB_H /* WARNING -- #include libprimer3.h, not this file */ /* The seq_lib struct represents a library of sequences. */ /* Clients do not need to know the details of this structure. */ typedef struct seq_lib { char **names; /* An array of sequence names. */ char **seqs; /* An array of sequences. */ char **rev_compl_seqs;/* An array of reversed-complemented sequences. x->rev_compl_seqs[i] is the reverse complement of x->seqs[i], which lets us keep track of pairwise mispriming. See reverse_complement_seq_lib(). */ double *weight; /* An array of weights. */ char *repeat_file; /* The path of the file containing the library. */ pr_append_str error; /* Global error message if any. */ pr_append_str warning;/* Warning message. */ /* The number of names, sequences, and weights. */ int seq_num; /* The size of storage allocated for names, sequences, and weights */ int storage_size; } seq_lib; /* ======================================================= */ /* Functions for creating and destroying a seq_lib object. */ /* ======================================================= */ /* * Reads any file in fasta format and returns a newly allocated * seq_lib, lib. Sets lib.error to a non-empty string on any error * other than ENOMEM. Returns NULL on ENOMEM. */ seq_lib * read_and_create_seq_lib(const char *filename, const char* errfrag); void destroy_seq_lib(seq_lib *lib); /* number of sequences in a seq_lib* */ int seq_lib_num_seq(const seq_lib* lib); char * seq_lib_warning_data(const seq_lib *lib); int add_seq_and_rev_comp_to_seq_lib(seq_lib *sl, char *seq, char *seq_id_plus, const char *errfrag); #endif primer3-2.3.6/src/read_boulder.h0000664000077300007730000000724512231156245015455 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009,2010 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of primer3. Primer3 and the libprimer3 library are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this file (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #ifndef BOULDER_INPUT_H #define BOULDER_INPUT_H 1 #include "libprimer3.h" typedef enum p3_file_type { all_parameters = 0, sequence = 1, settings = 2, } p3_file_type; typedef struct read_boulder_record_results { int explain_flag; int file_flag; } read_boulder_record_results; extern char *thermodynamic_params_path; /* path to thermodynamic parameter files */ extern int thermodynamic_path_changed;/* if this is set to 1, we need to re-read the thermodynamic parameters from new path */ /* * Read data from file_input until a "=" line occurs. Assign * parameter values for primer picking to pa and sarg. Perform initial * data checking. Return 0 if no records or no _more_ records were * found, and 1 otherwise. If nonfatal_err->data is not NULL or * fatal_err->data is not NULL then the data is erroneous and should * not be processed. Echo the input lines to stdout. */ int read_boulder_record(FILE *file_input, const int *strict_tags, const int * io_version, int echo_output, const p3_file_type read_file_type, p3_global_settings *pa, seq_args *sarg, pr_append_str *fatal_err, pr_append_str *nonfatal_err, pr_append_str *warnings, read_boulder_record_results *); /* Return null on error. */ /* pr_append_str is an append-only string ADT. */ int read_p3_file(const char *file_name, const p3_file_type file_type, int echo_output, int strict_tags, p3_global_settings *pa, seq_args *sarg, pr_append_str *fatal_err, pr_append_str *nonfatal_err, pr_append_str *warnings, read_boulder_record_results *read_boulder_record_res); #endif primer3-2.3.6/src/p3_seq_lib.c0000664000077300007730000003141712231156245015037 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of primer3 and primer3 software suite. Primer3 and the primer3 software suite are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this file (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include /* toupper() ... */ #include /* memset(), ... */ #include /* longjmp(), ... */ #include "libprimer3.h" /* Must include libprimer3.h, which includes p3_seq_lib.h */ static void *p3sl_safe_malloc(size_t x); static void *p3sl_safe_realloc(void *p, size_t x); static void p3sl_append_new_chunk(pr_append_str *x, const char *s); static void p3sl_append(pr_append_str *x, const char *s); static int add_seq_to_seq_lib(seq_lib *sl, char *seq, char *seq_id_plus, const char *errfrag); static jmp_buf _jmp_buf; #define P3SL_INIT_BUF_SIZE 1024 #define INIT_LIB_SIZE 500 #define PR_MAX_LIBRARY_WT 100.0 static double parse_seq_name(char *s); static char upcase_and_check_char(char *s); static void reverse_complement_seq_lib(seq_lib *lib); static int add_seq_to_seq_lib(seq_lib *sl, char *seq, char *seq_id_plus, const char *errfrag) { int i = sl->seq_num; int ss = sl->storage_size; char offender; char buf[2]; /* We need to allocate more storage */ if (i >= ss) { ss += INIT_LIB_SIZE; sl->storage_size = ss; sl->names = (char**) p3sl_safe_realloc(sl->names, ss*sizeof(*sl->names)); sl->seqs = (char**) p3sl_safe_realloc(sl->seqs , ss*sizeof(*sl->seqs)); /* sl->rev_compl_seqs = p3sl_safe_realloc(sl->seqs , ss*sizeof(*sl->rev_compl_seqs)); */ sl->weight= (double*) p3sl_safe_realloc(sl->weight, ss*sizeof(*sl->weight)); } sl->seq_num = i + 1; sl->names[i] = (char*) p3sl_safe_malloc(strlen(seq_id_plus) + 1); strcpy(sl->names[i], seq_id_plus); sl->weight[i] = parse_seq_name(sl->names[i]); if(sl->weight[i] < 0) { p3sl_append_new_chunk(&sl->error, "Illegal weight"); return 1; } /* sl->rev_compl_seqs[i] = */ sl->seqs[i] = (char*) p3sl_safe_malloc(strlen(seq) + 1); strcpy(sl->seqs[i], seq); if(strlen(sl->seqs[i]) == 0) { p3sl_append_new_chunk(&sl->error, "Empty sequence in "); return 1; } offender = upcase_and_check_char(sl->seqs[i]); if ('\0' != offender) { buf[0] = offender; buf[1] = '\0'; p3sl_append(&sl->warning, "Unrecognized character ("); p3sl_append(&sl->warning, buf); p3sl_append(&sl->warning, ") in "); p3sl_append(&sl->warning, errfrag); p3sl_append(&sl->warning, ", entry "); p3sl_append(&sl->warning, seq_id_plus); } return 0; } int add_seq_and_rev_comp_to_seq_lib(seq_lib *sl, char *seq, char *seq_id_plus, const char *errfrag) { char *rev_seq = NULL; char *rev_seq_id = NULL; int save_r = 0; if (seq == NULL) { save_r = 1; } if (add_seq_to_seq_lib(sl, seq, seq_id_plus, errfrag)) { return 1; } rev_seq_id = (char*) malloc(strlen(seq_id_plus) + 9); if (rev_seq_id == NULL) return 1; /* Handle the ID */ strcpy(rev_seq_id, "reverse "); strcat(rev_seq_id, seq_id_plus); /* Handle the sequence */ rev_seq = (char*) malloc(strlen(seq) + 1); if (rev_seq == NULL) { free(rev_seq_id); return 1; } p3_reverse_complement(seq, rev_seq); save_r = add_seq_to_seq_lib(sl, rev_seq, rev_seq_id, errfrag); free(rev_seq_id); free(rev_seq); return save_r; } seq_lib * create_empty_seq_lib() { seq_lib *lib; if (setjmp(_jmp_buf) != 0) return NULL; /* If we get here, there was an error in p3sl_safe_malloc or p3sl_safe_realloc. */ lib = (seq_lib*) p3sl_safe_malloc(sizeof(* lib)); memset(lib, 0, sizeof(*lib)); lib->repeat_file = NULL; lib->names = (char**) p3sl_safe_malloc(INIT_LIB_SIZE*sizeof(*lib->names)); lib->seqs = (char**) p3sl_safe_malloc(INIT_LIB_SIZE*sizeof(*lib->seqs)); /* FIX ME Can we get rid of rev_compl_seqs? */ /* lib->rev_compl_seqs = p3sl_safe_malloc(INIT_LIB_SIZE*sizeof(*lib->seqs)); */ lib->weight = (double*) p3sl_safe_malloc(INIT_LIB_SIZE*sizeof(*lib->weight)); lib->seq_num = 0; lib->storage_size = INIT_LIB_SIZE; return lib; } /* See comments in p3_seq_lib.h */ seq_lib * read_and_create_seq_lib(const char * filename, const char *errfrag) { char *p; FILE *file; char *seq_id_plus = NULL; char *seq = NULL; size_t seq_storage_size; size_t seq_len; seq_lib *lib = create_empty_seq_lib(); if (NULL == lib) return NULL; /* ENOMEM */ if (setjmp(_jmp_buf) != 0) return NULL; /* If we get here, there was an error in p3sl_safe_malloc or p3sl_safe_realloc. */ lib->repeat_file = (char*) p3sl_safe_malloc(strlen(filename) + 1); strcpy(lib->repeat_file, filename); if((file = fopen(lib->repeat_file,"r")) == NULL) { p3sl_append_new_chunk(&lib->error, "Cannot open "); goto ERROR; } seq = (char*) p3sl_safe_malloc(P3SL_INIT_BUF_SIZE); seq_storage_size = P3SL_INIT_BUF_SIZE; seq_len = 0; *seq = '\0'; /* Read in the lines from the file */ while(1) { p = p3_read_line(file); if (NULL == p) { /* End of file */ if (seq_id_plus != NULL) { if (seq_len == 0) { p3sl_append_new_chunk(&lib->error, "Empty sequence in "); goto ERROR; } else { if (add_seq_to_seq_lib(lib, seq, seq_id_plus, errfrag)) { p3sl_append(&lib->error, " in "); goto ERROR; } } free(seq_id_plus); seq_id_plus = NULL; } break; } if ('>' == *p) { /* We found an id line */ /* There are two possibilities */ if (seq_id_plus == NULL) { /* 1. This is the first id line in the file, in which case seq_id_plus == NULL (and seq_len == 0). */ seq_id_plus = (char*) p3sl_safe_malloc(strlen(p) + 1); p++; /* skip past the '>' */ strcpy(seq_id_plus, p); } else { /* 2. This is NOT the first id line in the file, in which case seq_id_plus != NULL */ if (seq_id_plus != NULL) { if (seq_len == 0) { p3sl_append_new_chunk(&lib->error, "Empty sequence in "); goto ERROR; } else { if (add_seq_to_seq_lib(lib, seq, seq_id_plus, errfrag)) { p3sl_append(&lib->error, " in "); goto ERROR; } /*"emtpy" the buffer */ seq_len = 0; *seq = '\0'; } free(seq_id_plus); seq_id_plus = (char*) p3sl_safe_malloc(strlen(p)); p++; /* skip past the '>' */ strcpy(seq_id_plus, p); } } } else { /* A sequence line */ if (seq_id_plus == NULL) { p3sl_append_new_chunk(&lib->error, "Missing id line (expected '>') in "); goto ERROR; } while ( strlen(p)+ seq_len + 1 > seq_storage_size ) { seq_storage_size *= 2; seq = (char*) p3sl_safe_realloc(seq, seq_storage_size); } strcat(seq, p); seq_len += strlen(p); } } if (lib->seq_num == 0) { p3sl_append_new_chunk(&lib->error, "Empty "); goto ERROR; } reverse_complement_seq_lib(lib); if (file) fclose(file); free(seq); free(seq_id_plus); return lib; ERROR: free(seq); free(seq_id_plus); p3sl_append(&lib->error, errfrag); p3sl_append(&lib->error, " "); p3sl_append(&lib->error, lib->repeat_file); if (file) fclose(file); return lib; } /* * Free exogenous storage associated with a seq_lib (but not the seq_lib * itself). Silently ignore NULL p. Set *p to 0 bytes. */ void destroy_seq_lib(seq_lib *p) { int i; if (NULL == p) return; free(p->repeat_file); if (NULL != p->seqs) { for(i = 0; i < p->seq_num; i++) if (NULL != p->seqs[i]) free(p->seqs[i]); free(p->seqs); } if (NULL != p->names) { for(i = 0; i < p->seq_num; i++) if (NULL != p->names[i]) free(p->names[i]); free(p->names); } free(p->weight); free(p->error.data); free(p->warning.data); free(p->rev_compl_seqs); free(p); } static void reverse_complement_seq_lib(seq_lib *lib) { int i, n, k; if((n = lib->seq_num) == 0) return; else { lib->names = (char**) p3sl_safe_realloc(lib->names, 2*n*sizeof(*lib->names)); lib->seqs = (char**) p3sl_safe_realloc(lib->seqs, 2*n*sizeof(*lib->seqs)); lib->weight = (double*) p3sl_safe_realloc(lib->weight, 2*n*sizeof(*lib->weight)); lib->rev_compl_seqs = (char**) p3sl_safe_malloc(2*n*sizeof(*lib->seqs)); lib->seq_num *= 2; for(i=n; iseq_num; i++){ k = strlen(lib->names[i-n]); lib->names[i] = (char*) p3sl_safe_malloc(k + 9); strcpy(lib->names[i], "reverse "); strcat(lib->names[i], lib->names[i-n]); lib->seqs[i] = (char*) p3sl_safe_malloc(strlen(lib->seqs[i-n]) + 1); p3_reverse_complement(lib->seqs[i-n], lib->seqs[i]); lib->weight[i] = lib->weight[i-n]; lib->rev_compl_seqs[i-n] = lib->seqs[i]; lib->rev_compl_seqs[i] = lib->seqs[i-n]; } } return; } int seq_lib_num_seq(const seq_lib* lib) { if (NULL == lib) return 0; else return lib->seq_num; } char * seq_lib_warning_data(const seq_lib *lib) { if (NULL == lib) return NULL; else return lib->warning.data; } static double parse_seq_name(char *s) { char *p, *q; double n; p = s; while( *p != '*' && *p != '\0' ) p++; if (*p == '\0' ) return 1; else { p++; n = strtod( p, &q ); if( q == p ) return -1; } if(n > PR_MAX_LIBRARY_WT) return -1; return n; } /* * Removes spaces and "end-of-line" characters * from the sequence, replaces all other * characters except A, T, G, C and IUB/IUPAC * codes with N. Returns 0 if there were no such * replacements and the first non-ACGT, non-IUB * character otherwise. */ static char upcase_and_check_char(char *s) { int i, j, n, m; j = 0; m = 0; n = strlen(s); for(i=0; i /* INT_MIN */ #ifdef __cplusplus extern "C" { #endif #define DPAL_ERROR_SCORE INT_MIN #define DPAL_EXIT_ON_ERROR 0 /* 0 means do not exit on error. */ #ifndef DPAL_MAX_ALIGN #define DPAL_MAX_ALIGN 1600 /* * The maximum size of a string that can be * aligned with with generic dpal and for which * we can return a "path". Several arrays of * size DPAL_MAX_ALIGN X DPAL_MAX_ALIGN are * statically allocated in dpal.o */ #endif #define DPAL_LOCAL 0 /* Return a local alignment. */ #define DPAL_GLOBAL_END 1 /* * Return a global alignment _anchored at the end * of the first sequence_. */ #define DPAL_GLOBAL 2 /* * Return an arbitrary global alignment, that is * one anchored at the end of either the first or * the second sequence. */ #define DPAL_LOCAL_END 3 /* * Return a local alignment that includes the * end (but not necessarily the beginning) of * the first sequence. */ /* * It is not possible to specify end-gap penalties for the DPAL_GLOBAL_END * and DPLAL_GLOBAL flags. */ /* * The data structure that stores the "scoring system matrix". (The socring * system matrix data structure is of size UCHAR_MAX + 1 by UCHAR_MAX + 1.) */ typedef int dpal_ssm[UCHAR_MAX + 1][UCHAR_MAX + 1]; /* Structure for passing in arguments to the main function, dpal. */ typedef struct { int check_chars; /* * If non-0, check for and raise an error on an * illegal character in the input strings. */ int debug; /* * If non-0, print debugging information to * stderr. */ int fail_stop; /* Exit with -1 on error. */ int flag; /* * One of DPAL_GLOBAL, DPAL_LOCAL, * DPAL_GLOBAL_END, DPAL_LOCAL_END */ int force_generic; /* Force the use of the generic function. */ int force_long_generic; /* * Force the use of the long generic no-path * function. */ int force_long_maxgap1; /* Force the use of the long maxgap 1 functions. */ int gap; /* The "gap opening" penalty. */ int gapl; /* The "gap extension" penalty. */ int max_gap; /* * The maximum allowable size for a gap. -1 * indicates that the gap can be of any size. */ int score_max; /* If greater than 0 stop search as soon as * score > score_max. */ int score_only; /* * If non-0, only print the score on * stdout. (Incompatible with debug.) */ dpal_ssm ssm; /* The scoring system matrix. */ } dpal_args; /* Structure for receiving results from the main function, dpal. */ typedef struct { const char *msg; int path[DPAL_MAX_ALIGN][2]; int path_length; int align_end_1; /* Last alignment position in the 1st sequence. */ int align_end_2; /* Last alignment position in the 2nd sequence. */ double score; } dpal_results; /* Initialize the argument to the default matrix for nucleotide matches. */ void dpal_set_default_nt_args(dpal_args *); /* Routine primarily for testing: sets CC & GG matches to 3, AA & TT matches to 2. */ void dpal_set_h_nt_matrix(dpal_args *a); /* The argument, a, must be a DNA scoring matrix. Modifies a so that it for a match between any two ambiguity codes (or between ambiguity code and base), e.g. B and S, the score will be the maximum of score between any base in B and any base in S, in the example between any pair in {C, G, T} X {C, G}. This function overwrites any scores already associated with pairs of ambiguity codes. Return 0 on error, 1 on success. */ int dpal_set_ambiguity_code_matrix(dpal_args *); /* * Align the first 2 arguments, using the scoring * matix and other arguments supplied in the dpal_args * argument. Return results in argument 'out'. * On error, sets out->score to DPAL_ERROR_SCORE * and puts additional information in out->msg. */ void dpal(const unsigned char *, const unsigned char*, const dpal_args *, dpal_results *out); void set_dpal_args(dpal_args *); #ifdef __cplusplus } #endif #endif primer3-2.3.6/src/read_boulder.c0000664000077300007730000013575212231156245015455 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009, 2010,2011,2012 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky. All rights reserved. This file is part of primer3 and the primer3 suite. Primer3 and the primer3 suite are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this file (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include #include /* strtod, strtol,... */ #include /* toupper, isspace */ #include /* memset, strlen, strcmp, ... */ #include #include "read_boulder.h" #define INIT_BUF_SIZE 1024 #define INIT_LIB_SIZE 500 #define PR_MAX_LIBRARY_WT 100.0 char *thermodynamic_params_path = NULL; int thermodynamic_path_changed = 1; /* Static functions. */ static void *_rb_safe_malloc(size_t x); static void out_of_memory_error(); static void parse_double(const char *, const char *, double *, pr_append_str *); static void parse_int(const char *, const char *, int *, pr_append_str *); static const char *parse_int_pair(const char *, const char *, char, int *, int *, pr_append_str *); static char *parse_2_int_pair(const char*, char*, char, char, int*, int*, int*, int*, pr_append_str*); static void parse_interval_list(const char *tag_name, const char *datum, interval_array_t2 *interval_arr, pr_append_str *err); static void parse_2_interval_list(const char *tag_name, char *datum, interval_array_t4 *interval_arr, pr_append_str *err); static int parse_intron_list(char *, int *, int *); static void parse_product_size(const char *, char *, p3_global_settings *, pr_append_str *); static void pr_append(pr_append_str *, const char *); static void pr_append_new_chunk(pr_append_str *x, const char *s); static void tag_syntax_error(const char *, const char *, pr_append_str *); static int parse_seq_quality(char *, seq_args *); static const char *pr_program_name = "TMP"; /* * Hack to support old SunOS headers. (We do not try to declare _all_ * undeclared functions; only those with non-int return types.) */ #ifndef __cplusplus extern double strtod(); #endif /* * See read_boulder.h for description. */ #define COMPARE(TAG) (!strncmp(s, TAG, tag_len) \ && ('=' == s[tag_len] || ' ' == s[tag_len]) \ && '\0' == TAG[tag_len]) #define COMPARE_AND_MALLOC(TAG,T) \ if (COMPARE(TAG)) { \ if (T) { \ pr_append_new_chunk(parse_err, \ "Duplicate tag: "); \ pr_append(parse_err, TAG); \ } else { \ T = (char*) _rb_safe_malloc(datum_len + 1); \ strcpy(T, datum); \ } \ continue; \ } #define OVERWRITE_COMPARE_AND_MALLOC(TAG,T) \ if (COMPARE(TAG)) { \ if (T) { \ free(T); \ } \ T = (char*) _rb_safe_malloc(datum_len + 1); \ strcpy(T, datum); \ continue; \ } #define COMPARE_FLOAT(TAG,T) \ if (COMPARE(TAG)) { \ parse_double(TAG, datum, &(T), parse_err); \ continue; \ } /* This macro added 2011 08 28 to allow use of of the 'setting' functions, such as p3_set_gs_primer_self_end, in setting arguments. At this point, this macro is only used twice, and there are no analogous macros for integers,etc. */ #define COMPARE_FLOAT_USE_FN(TAG, set_function) \ if (COMPARE(TAG)) { \ parse_double(TAG, datum, &tmp_double, parse_err); \ set_function(pa, tmp_double); \ continue; \ } #define COMPARE_INT(TAG,T) \ if (COMPARE(TAG)) { \ parse_int(TAG, datum, &(T), parse_err); \ continue; \ } #define COMPARE_INTERVAL_LIST(TAG, PLACE) \ if (COMPARE(TAG)) { \ parse_interval_list(TAG, datum, PLACE, parse_err); \ continue; \ } #define COMPARE_2_INTERVAL_LIST(TAG, PLACE) \ if (COMPARE(TAG)) { \ parse_2_interval_list(TAG, datum, PLACE, parse_err);\ continue; \ } /* c-basic-offset in emacs is set to 2 */ /* * See read_boulder.h for description. */ int read_boulder_record(FILE *file_input, const int *strict_tags, const int *io_version, int echo_output, /* should be echo_input */ const p3_file_type file_type, p3_global_settings *pa, seq_args *sa, pr_append_str *glob_err, /* Really should be called fatal_parse_err */ pr_append_str *nonfatal_parse_err, pr_append_str *warnings, read_boulder_record_results *res) { int line_len; int tag_len, datum_len; int data_found = 0; char *s, *n, *datum, *task_tmp = NULL; const char *p; pr_append_str *parse_err; pr_append_str *non_fatal_err; char *repeat_file_path = NULL, *int_repeat_file_path = NULL; int tmp_int; /* int min_3_prime = 0, min_5_prime = 0; Removed 10/20/2010 */ int min_3_prime_distance_global = 0; /* needed to check if both global and specific*/ int min_3_prime_distance_specific = 0; /* are given in same boulder record */ int min_three_prime_distance; /* holder for the value of this tag */ double tmp_double; non_fatal_err = nonfatal_parse_err; while (((s = p3_read_line(file_input)) != NULL) && (strcmp(s,"="))) { /* If we are reading from a settings file, then skip every line except those begining "PRIMER_" or "P3_FILE_ID". Hint: strncomp returns 0 if both strings are equal */ if (file_type == settings && strncmp(s, "PRIMER_", 7) /* "s does not begin with 'PRIMER_'" */ && strncmp(s, "P3_FILE_ID", 10) /* "s does not begin with 'P3_FILE_ID'" */ ) { continue; } /* Silently ignore all primer3plus tags */ /* Removed 10/20/10 because P3P_ tags are already ignored in settings files. */ /* if (!(strncmp(s, "P3P_", 4))) { continue; } */ data_found = 1; /* Print out the input */ if (echo_output) printf("%s\n", s); line_len = strlen(s); /* If the line has an "=" read the tag in the right place */ if ((n=strchr(s,'=')) == NULL) { /* The input line is illegal because it has no * "=" in it, but we still will read to the end * of the record. */ pr_append_new_chunk(glob_err, "Input line with no '=': "); pr_append(glob_err, s); continue; } /* Read in the new tags used from primer3 version 2.0 on */ else { /* Get the tag and the value pointers */ tag_len = n - s; datum = n + 1; datum_len = line_len - tag_len - 1; /* Process "Sequence" (i.e. Per-Record) Arguments". */ parse_err = non_fatal_err; /* COMPARE_AND_MALLOC("SEQUENCE", sa->sequence); */ if (COMPARE("SEQUENCE_TEMPLATE")) { /* NEW WAY */ if (/* p3_get_seq_arg_sequence(sa) */ sa->sequence) { pr_append_new_chunk(parse_err, "Duplicate tag: "); pr_append(parse_err, "SEQUENCE_TEMPLATE"); } else { if (p3_set_sa_sequence(sa, datum)) exit(-2); } continue; } if (COMPARE("SEQUENCE_QUALITY")) { if ((sa->n_quality = parse_seq_quality(datum, sa)) == 0) { pr_append_new_chunk(parse_err, "Error in sequence quality data"); } continue; } COMPARE_AND_MALLOC("SEQUENCE_ID", sa->sequence_name); COMPARE_AND_MALLOC("SEQUENCE_PRIMER", sa->left_input); COMPARE_AND_MALLOC("SEQUENCE_PRIMER_REVCOMP", sa->right_input); COMPARE_AND_MALLOC("SEQUENCE_INTERNAL_OLIGO", sa->internal_input); COMPARE_2_INTERVAL_LIST("SEQUENCE_PRIMER_PAIR_OK_REGION_LIST", &sa->ok_regions); COMPARE_INTERVAL_LIST("SEQUENCE_TARGET", &sa->tar2); COMPARE_INTERVAL_LIST("SEQUENCE_EXCLUDED_REGION", &sa->excl2); COMPARE_INTERVAL_LIST("SEQUENCE_INTERNAL_EXCLUDED_REGION", &sa->excl_internal2); if (COMPARE("SEQUENCE_OVERLAP_JUNCTION_LIST")) { if (parse_intron_list(datum, sa->primer_overlap_junctions, &sa->primer_overlap_junctions_count) == 0) { pr_append_new_chunk(parse_err, "Error in SEQUENCE_PRIMER_OVERLAP_JUNCTION_LIST"); } continue; } if (COMPARE("SEQUENCE_INCLUDED_REGION")) { p = parse_int_pair("SEQUENCE_INCLUDED_REGION", datum, ',', &sa->incl_s, &sa->incl_l, parse_err); if (NULL == p) /* An error; the message is already * in parse_err. */ continue; while (' ' == *p || '\t' == *p) p++; if (*p != '\n' && *p != '\0') tag_syntax_error("SEQUENCE_INCLUDED_REGION", datum, parse_err); continue; } COMPARE_INT("SEQUENCE_START_CODON_POSITION", sa->start_codon_pos); COMPARE_INT("SEQUENCE_FORCE_LEFT_START", sa->force_left_start); COMPARE_INT("SEQUENCE_FORCE_LEFT_END", sa->force_left_end); COMPARE_INT("SEQUENCE_FORCE_RIGHT_START", sa->force_right_start); COMPARE_INT("SEQUENCE_FORCE_RIGHT_END", sa->force_right_end); /* Process "Global" Arguments (those that persist between boulder * records). */ parse_err = glob_err; /* These errors are considered fatal. */ if (COMPARE("PRIMER_PRODUCT_SIZE_RANGE")) { parse_product_size("PRIMER_PRODUCT_SIZE_RANGE", datum, pa, parse_err); continue; } COMPARE_INT("PRIMER_OPT_SIZE", pa->p_args.opt_size); COMPARE_INT("PRIMER_MIN_SIZE", pa->p_args.min_size); COMPARE_INT("PRIMER_MAX_SIZE", pa->p_args.max_size); COMPARE_INT("PRIMER_MAX_POLY_X", pa->p_args.max_poly_x); COMPARE_FLOAT("PRIMER_OPT_TM", pa->p_args.opt_tm); COMPARE_FLOAT("PRIMER_OPT_GC_PERCENT", pa->p_args.opt_gc_content); COMPARE_FLOAT("PRIMER_MIN_TM", pa->p_args.min_tm); COMPARE_FLOAT("PRIMER_MAX_TM", pa->p_args.max_tm); COMPARE_FLOAT("PRIMER_PAIR_MAX_DIFF_TM", pa->max_diff_tm); if (COMPARE("PRIMER_TM_FORMULA")) { parse_int("PRIMER_TM_FORMULA", datum, &tmp_int, parse_err); pa->tm_santalucia = (tm_method_type) tmp_int; /* added by T.Koressaar */ continue; } if (COMPARE("PRIMER_SALT_CORRECTIONS")) { parse_int("PRIMER_SALT_CORRECTIONS", datum, &tmp_int, parse_err); pa->salt_corrections = (salt_correction_type) tmp_int; /* added by T.Koressaar */ continue; } COMPARE_FLOAT("PRIMER_MIN_GC", pa->p_args.min_gc); COMPARE_FLOAT("PRIMER_MAX_GC", pa->p_args.max_gc); COMPARE_FLOAT("PRIMER_SALT_MONOVALENT", pa->p_args.salt_conc); COMPARE_FLOAT("PRIMER_SALT_DIVALENT", pa->p_args.divalent_conc); COMPARE_FLOAT("PRIMER_DNTP_CONC", pa->p_args.dntp_conc); COMPARE_FLOAT("PRIMER_DNA_CONC", pa->p_args.dna_conc); COMPARE_INT("PRIMER_MAX_NS_ACCEPTED", pa->p_args.num_ns_accepted); COMPARE_INT("PRIMER_PRODUCT_OPT_SIZE", pa->product_opt_size); COMPARE_FLOAT("PRIMER_MAX_SELF_ANY", pa->p_args.max_self_any); /* COMPARE_FLOAT("PRIMER_MAX_SELF_END", pa->p_args.max_self_end); */ /* NEW */ COMPARE_FLOAT_USE_FN("PRIMER_MAX_SELF_END", p3_set_gs_primer_self_end) /* if (COMPARE("PRIMER_MAX_SELF_END")) { parse_double("PRIMER_MAX_SELF_END", datum, &tmp_double, parse_err); p3_set_gs_primer_self_end(pa, tmp_double); continue; } */ COMPARE_FLOAT("PRIMER_MAX_SELF_ANY_TH", pa->p_args.max_self_any_th); COMPARE_FLOAT("PRIMER_MAX_SELF_END_TH", pa->p_args.max_self_end_th); COMPARE_FLOAT("PRIMER_MAX_HAIRPIN_TH", pa->p_args.max_hairpin_th); COMPARE_FLOAT("PRIMER_PAIR_MAX_COMPL_ANY", pa->pair_compl_any); COMPARE_FLOAT("PRIMER_PAIR_MAX_COMPL_END", pa->pair_compl_end); COMPARE_FLOAT("PRIMER_PAIR_MAX_COMPL_ANY_TH", pa->pair_compl_any_th); COMPARE_FLOAT("PRIMER_PAIR_MAX_COMPL_END_TH", pa->pair_compl_end_th); COMPARE_INT("P3_FILE_FLAG", res->file_flag); COMPARE_INT("PRIMER_PICK_ANYWAY", pa->pick_anyway); COMPARE_INT("PRIMER_GC_CLAMP", pa->gc_clamp); COMPARE_INT("PRIMER_MAX_END_GC", pa->max_end_gc); COMPARE_INT("PRIMER_EXPLAIN_FLAG", res->explain_flag); COMPARE_INT("PRIMER_LIBERAL_BASE", pa->liberal_base); COMPARE_INT("PRIMER_FIRST_BASE_INDEX", pa->first_base_index); COMPARE_INT("PRIMER_NUM_RETURN", pa->num_return); COMPARE_INT("PRIMER_MIN_QUALITY", pa->p_args.min_quality); COMPARE_INT("PRIMER_MIN_END_QUALITY", pa->p_args.min_end_quality); if (COMPARE("PRIMER_MIN_THREE_PRIME_DISTANCE")) { parse_int("PRIMER_MIN_THREE_PRIME_DISTANCE", datum, &(min_three_prime_distance), parse_err); /* check if specific tag also specified - error in this case */ if (min_3_prime_distance_specific == 1) { pr_append_new_chunk(glob_err, "Both PRIMER_MIN_THREE_PRIME_DISTANCE and PRIMER_{LEFT/RIGHT}_MIN_THREE_PRIME_DISTANCE specified"); } else { min_3_prime_distance_global = 1; /* set up individual flags */ pa->min_left_three_prime_distance = min_three_prime_distance; pa->min_right_three_prime_distance = min_three_prime_distance; } continue; } if (COMPARE("PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE")) { parse_int("PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE", datum, &(pa->min_left_three_prime_distance), parse_err); /* check if global tag also specified - error in this case */ if (min_3_prime_distance_global == 1) { pr_append_new_chunk(glob_err, "Both PRIMER_MIN_THREE_PRIME_DISTANCE and PRIMER_{LEFT/RIGHT}_MIN_THREE_PRIME_DISTANCE specified"); } else { min_3_prime_distance_specific = 1; } continue; } if (COMPARE("PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE")) { parse_int("PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE", datum, &(pa->min_right_three_prime_distance), parse_err); /* check if global tag also specified - error in this case */ if (min_3_prime_distance_global == 1) { pr_append_new_chunk(glob_err, "Both PRIMER_MIN_THREE_PRIME_DISTANCE and PRIMER_{LEFT/RIGHT}_MIN_THREE_PRIME_DISTANCE specified"); } else { min_3_prime_distance_specific = 1; } continue; } if (file_type == settings) { if (COMPARE("P3_FILE_ID")) continue; } COMPARE_INT("PRIMER_QUALITY_RANGE_MIN", pa->quality_range_min); COMPARE_INT("PRIMER_QUALITY_RANGE_MAX", pa->quality_range_max); COMPARE_FLOAT("PRIMER_PRODUCT_MAX_TM", pa->product_max_tm); COMPARE_FLOAT("PRIMER_PRODUCT_MIN_TM", pa->product_min_tm); COMPARE_FLOAT("PRIMER_PRODUCT_OPT_TM", pa->product_opt_tm); COMPARE_INT("PRIMER_SEQUENCING_LEAD", pa->sequencing.lead); COMPARE_INT("PRIMER_SEQUENCING_SPACING", pa->sequencing.spacing); COMPARE_INT("PRIMER_SEQUENCING_INTERVAL", pa->sequencing.interval); COMPARE_INT("PRIMER_SEQUENCING_ACCURACY", pa->sequencing.accuracy); if (COMPARE("PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION")) { parse_int("PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION", datum, &pa->min_5_prime_overlap_of_junction, parse_err); /* min_5_prime = 1; Removed 10/20/2010 */ continue; } if (COMPARE("PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION")) { parse_int("PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION", datum, &pa->min_3_prime_overlap_of_junction, parse_err); /* min_3_prime = 1; Removed 10/20/2010 */ continue; } COMPARE_AND_MALLOC("PRIMER_TASK", task_tmp); COMPARE_INT("PRIMER_PICK_RIGHT_PRIMER", pa->pick_right_primer); COMPARE_INT("PRIMER_PICK_INTERNAL_OLIGO", pa->pick_internal_oligo); COMPARE_INT("PRIMER_PICK_LEFT_PRIMER", pa->pick_left_primer); COMPARE_INT("PRIMER_INTERNAL_OPT_SIZE", pa->o_args.opt_size); COMPARE_INT("PRIMER_INTERNAL_MAX_SIZE", pa->o_args.max_size); COMPARE_INT("PRIMER_INTERNAL_MIN_SIZE", pa->o_args.min_size); COMPARE_INT("PRIMER_INTERNAL_MAX_POLY_X", pa->o_args.max_poly_x); COMPARE_FLOAT("PRIMER_INTERNAL_OPT_TM", pa->o_args.opt_tm); COMPARE_FLOAT("PRIMER_INTERNAL_OPT_GC_PERCENT", pa->o_args.opt_gc_content); COMPARE_FLOAT("PRIMER_INTERNAL_MAX_TM", pa->o_args.max_tm); COMPARE_FLOAT("PRIMER_INTERNAL_MIN_TM", pa->o_args.min_tm); COMPARE_FLOAT("PRIMER_INTERNAL_MIN_GC", pa->o_args.min_gc); COMPARE_FLOAT("PRIMER_INTERNAL_MAX_GC", pa->o_args.max_gc); COMPARE_FLOAT("PRIMER_INTERNAL_SALT_MONOVALENT", pa->o_args.salt_conc); COMPARE_FLOAT("PRIMER_INTERNAL_SALT_DIVALENT", pa->o_args.divalent_conc); COMPARE_FLOAT("PRIMER_INTERNAL_DNTP_CONC", pa->o_args.dntp_conc); COMPARE_FLOAT("PRIMER_INTERNAL_DNA_CONC", pa->o_args.dna_conc); COMPARE_INT("PRIMER_INTERNAL_MAX_NS_ACCEPTED", pa->o_args.num_ns_accepted); COMPARE_INT("PRIMER_INTERNAL_MIN_QUALITY", pa->o_args.min_quality); COMPARE_FLOAT("PRIMER_INTERNAL_MAX_SELF_ANY", pa->o_args.max_self_any); /* COMPARE_FLOAT("PRIMER_INTERNAL_MAX_SELF_END", pa->o_args.max_self_end); */ /* NEW */ COMPARE_FLOAT_USE_FN("PRIMER_INTERNAL_MAX_SELF_END", p3_set_gs_primer_internal_oligo_self_end); /* if (COMPARE("PRIMER_INTERNAL_MAX_SELF_END")) { parse_double("PRIMER_INTERNAL_MAX_SELF_END", datum, &tmp_double, parse_err); p3_set_gs_primer_internal_oligo_self_end(pa, tmp_double); continue; } */ COMPARE_FLOAT("PRIMER_INTERNAL_MAX_SELF_ANY_TH", pa->o_args.max_self_any_th); COMPARE_FLOAT("PRIMER_INTERNAL_MAX_SELF_END_TH", pa->o_args.max_self_end_th); COMPARE_FLOAT("PRIMER_INTERNAL_MAX_HAIRPIN_TH", pa->o_args.max_hairpin_th); COMPARE_FLOAT("PRIMER_MAX_LIBRARY_MISPRIMING", pa->p_args.max_repeat_compl); COMPARE_FLOAT("PRIMER_INTERNAL_MAX_LIBRARY_MISHYB", pa->o_args.max_repeat_compl); COMPARE_FLOAT("PRIMER_PAIR_MAX_LIBRARY_MISPRIMING", pa->pair_repeat_compl); /* Mispriming / mishybing in the template. */ COMPARE_FLOAT("PRIMER_MAX_TEMPLATE_MISPRIMING", pa->p_args.max_template_mispriming); COMPARE_FLOAT("PRIMER_MAX_TEMPLATE_MISPRIMING_TH", pa->p_args.max_template_mispriming_th); COMPARE_FLOAT("PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING", pa->pair_max_template_mispriming); COMPARE_FLOAT("PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH", pa->pair_max_template_mispriming_th); /* Control interpretation of ambiguity codes in mispriming and mishyb libraries. */ COMPARE_INT("PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS", pa->lib_ambiguity_codes_consensus); COMPARE_FLOAT("PRIMER_INSIDE_PENALTY", pa->inside_penalty); COMPARE_FLOAT("PRIMER_OUTSIDE_PENALTY", pa->outside_penalty); if (COMPARE("PRIMER_MISPRIMING_LIBRARY")) { if (repeat_file_path != NULL) { pr_append_new_chunk(glob_err, "Duplicate PRIMER_MISPRIMING_LIBRARY tag"); free(repeat_file_path); repeat_file_path = NULL; } else { repeat_file_path = (char*) _rb_safe_malloc(strlen(datum) + 1); strcpy(repeat_file_path, datum); } continue; } if (COMPARE("PRIMER_INTERNAL_MISHYB_LIBRARY")) { if (int_repeat_file_path != NULL) { pr_append_new_chunk(glob_err, "Duplicate PRIMER_INTERNAL_MISHYB_LIBRARY tag"); free(int_repeat_file_path); int_repeat_file_path = NULL; } else { int_repeat_file_path = (char*) _rb_safe_malloc(strlen(datum) + 1); strcpy(int_repeat_file_path, datum); } continue; } if (COMPARE("P3_COMMENT")) continue; COMPARE_FLOAT("PRIMER_MAX_END_STABILITY", pa->max_end_stability); COMPARE_INT("PRIMER_LOWERCASE_MASKING", pa->lowercase_masking); /* added by T. Koressaar */ COMPARE_INT("PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT", pa->thermodynamic_oligo_alignment); COMPARE_INT("PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT", pa->thermodynamic_template_alignment); if (COMPARE("PRIMER_THERMODYNAMIC_PARAMETERS_PATH")) { if (thermodynamic_params_path == NULL) { thermodynamic_params_path = (char*) _rb_safe_malloc(datum_len + 1); strcpy(thermodynamic_params_path, datum); thermodynamic_path_changed = 1; } /* check if path changes */ else if (strcmp(thermodynamic_params_path, datum)) { free(thermodynamic_params_path); thermodynamic_params_path = (char*) _rb_safe_malloc(datum_len + 1); strcpy(thermodynamic_params_path, datum); thermodynamic_path_changed = 1; } continue; } /* added by A. Untergasser */ OVERWRITE_COMPARE_AND_MALLOC("PRIMER_MUST_MATCH_FIVE_PRIME", pa->p_args.must_match_five_prime); OVERWRITE_COMPARE_AND_MALLOC("PRIMER_MUST_MATCH_THREE_PRIME", pa->p_args.must_match_three_prime); OVERWRITE_COMPARE_AND_MALLOC("PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME", pa->o_args.must_match_five_prime); OVERWRITE_COMPARE_AND_MALLOC("PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME", pa->o_args.must_match_three_prime); /* weights for objective functions */ /* CHANGE TEMP/temp -> TM/tm */ COMPARE_FLOAT("PRIMER_WT_TM_GT", pa->p_args.weights.temp_gt); COMPARE_FLOAT("PRIMER_WT_TM_LT", pa->p_args.weights.temp_lt); COMPARE_FLOAT("PRIMER_WT_GC_PERCENT_GT", pa->p_args.weights.gc_content_gt); COMPARE_FLOAT("PRIMER_WT_GC_PERCENT_LT", pa->p_args.weights.gc_content_lt); COMPARE_FLOAT("PRIMER_WT_SIZE_LT", pa->p_args.weights.length_lt); COMPARE_FLOAT("PRIMER_WT_SIZE_GT", pa->p_args.weights.length_gt); COMPARE_FLOAT("PRIMER_WT_SELF_ANY", pa->p_args.weights.compl_any); COMPARE_FLOAT("PRIMER_WT_SELF_END", pa->p_args.weights.compl_end); COMPARE_FLOAT("PRIMER_WT_SELF_ANY_TH", pa->p_args.weights.compl_any_th); COMPARE_FLOAT("PRIMER_WT_SELF_END_TH", pa->p_args.weights.compl_end_th); COMPARE_FLOAT("PRIMER_WT_HAIRPIN_TH", pa->p_args.weights.hairpin_th); COMPARE_FLOAT("PRIMER_WT_NUM_NS", pa->p_args.weights.num_ns); COMPARE_FLOAT("PRIMER_WT_LIBRARY_MISPRIMING", pa->p_args.weights.repeat_sim); COMPARE_FLOAT("PRIMER_WT_SEQ_QUAL", pa->p_args.weights.seq_quality); COMPARE_FLOAT("PRIMER_WT_END_QUAL", pa->p_args.weights.end_quality); COMPARE_FLOAT("PRIMER_WT_POS_PENALTY", pa->p_args.weights.pos_penalty); COMPARE_FLOAT("PRIMER_WT_END_STABILITY", pa->p_args.weights.end_stability); COMPARE_FLOAT("PRIMER_WT_TEMPLATE_MISPRIMING", pa->p_args.weights.template_mispriming); COMPARE_FLOAT("PRIMER_WT_TEMPLATE_MISPRIMING_TH", pa->p_args.weights.template_mispriming_th); COMPARE_FLOAT("PRIMER_INTERNAL_WT_TM_GT", pa->o_args.weights.temp_gt); COMPARE_FLOAT("PRIMER_INTERNAL_WT_TM_LT", pa->o_args.weights.temp_lt); COMPARE_FLOAT("PRIMER_INTERNAL_WT_GC_PERCENT_GT", pa->o_args.weights.gc_content_gt); COMPARE_FLOAT("PRIMER_INTERNAL_WT_GC_PERCENT_LT", pa->o_args.weights.gc_content_lt); COMPARE_FLOAT("PRIMER_INTERNAL_WT_SIZE_LT", pa->o_args.weights.length_lt); COMPARE_FLOAT("PRIMER_INTERNAL_WT_SIZE_GT", pa->o_args.weights.length_gt); COMPARE_FLOAT("PRIMER_INTERNAL_WT_SELF_ANY", pa->o_args.weights.compl_any); COMPARE_FLOAT("PRIMER_INTERNAL_WT_SELF_END", pa->o_args.weights.compl_end); COMPARE_FLOAT("PRIMER_INTERNAL_WT_SELF_ANY_TH", pa->o_args.weights.compl_any_th); COMPARE_FLOAT("PRIMER_INTERNAL_WT_SELF_END_TH", pa->o_args.weights.compl_end_th); COMPARE_FLOAT("PRIMER_INTERNAL_WT_HAIRPIN_TH", pa->o_args.weights.hairpin_th); COMPARE_FLOAT("PRIMER_INTERNAL_WT_NUM_NS", pa->o_args.weights.num_ns); COMPARE_FLOAT("PRIMER_INTERNAL_WT_LIBRARY_MISHYB", pa->o_args.weights.repeat_sim); COMPARE_FLOAT("PRIMER_INTERNAL_WT_SEQ_QUAL", pa->o_args.weights.seq_quality); COMPARE_FLOAT("PRIMER_INTERNAL_WT_END_QUAL", pa->o_args.weights.end_quality); COMPARE_FLOAT("PRIMER_WT_TEMPLATE_MISPRIMING_TH", pa->o_args.weights.template_mispriming_th); COMPARE_FLOAT("PRIMER_PAIR_WT_PR_PENALTY", pa->pr_pair_weights.primer_quality); COMPARE_FLOAT("PRIMER_PAIR_WT_IO_PENALTY", pa->pr_pair_weights.io_quality); COMPARE_FLOAT("PRIMER_PAIR_WT_DIFF_TM", pa->pr_pair_weights.diff_tm); COMPARE_FLOAT("PRIMER_PAIR_WT_COMPL_ANY", pa->pr_pair_weights.compl_any); COMPARE_FLOAT("PRIMER_PAIR_WT_COMPL_END", pa->pr_pair_weights.compl_end); COMPARE_FLOAT("PRIMER_PAIR_WT_COMPL_ANY_TH", pa->pr_pair_weights.compl_any_th); COMPARE_FLOAT("PRIMER_PAIR_WT_COMPL_END_TH", pa->pr_pair_weights.compl_end_th); COMPARE_FLOAT("PRIMER_PAIR_WT_PRODUCT_TM_LT", pa->pr_pair_weights.product_tm_lt); COMPARE_FLOAT("PRIMER_PAIR_WT_PRODUCT_TM_GT", pa->pr_pair_weights.product_tm_gt); COMPARE_FLOAT("PRIMER_PAIR_WT_PRODUCT_SIZE_GT", pa->pr_pair_weights.product_size_gt); COMPARE_FLOAT("PRIMER_PAIR_WT_PRODUCT_SIZE_LT", pa->pr_pair_weights.product_size_lt); COMPARE_FLOAT("PRIMER_PAIR_WT_LIBRARY_MISPRIMING", pa->pr_pair_weights.repeat_sim); COMPARE_FLOAT("PRIMER_PAIR_WT_TEMPLATE_MISPRIMING", pa->pr_pair_weights.template_mispriming); COMPARE_FLOAT("PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH", pa->pr_pair_weights.template_mispriming_th); } /* End of reading the tags in the right place */ /* Complain about unrecognized tags */ if (*strict_tags == 1) { pr_append_new_chunk(glob_err, "Unrecognized tag: "); pr_append(glob_err, s); fprintf(stderr, "Unrecognized tag: %s\n", s); } } /* while ((s = p3_read_line(stdin)) != NULL && strcmp(s,"=")) { */ /* Check if the record was terminated by "=" */ if (NULL == s) { /* End of file. */ if (data_found) { pr_append_new_chunk(glob_err, "Final record not terminated by '='"); return 1; } else return 0; } /* Figure out the right settings for the tasks*/ if (task_tmp != NULL) { if (!strcmp_nocase(task_tmp, "pick_pcr_primers")) { pa->primer_task = generic; pa->pick_left_primer = 1; pa->pick_right_primer = 1; pa->pick_internal_oligo = 0; } else if (!strcmp_nocase(task_tmp, "pick_pcr_primers_and_hyb_probe")) { pa->primer_task = generic; pa->pick_left_primer = 1; pa->pick_right_primer = 1; pa->pick_internal_oligo = 1; } else if (!strcmp_nocase(task_tmp, "pick_left_only")) { pa->primer_task = generic; pa->pick_left_primer = 1; pa->pick_right_primer = 0; pa->pick_internal_oligo = 0; } else if (!strcmp_nocase(task_tmp, "pick_right_only")) { pa->primer_task = generic; pa->pick_left_primer = 0; pa->pick_right_primer = 1; pa->pick_internal_oligo = 0; } else if (!strcmp_nocase(task_tmp, "pick_hyb_probe_only")) { pa->primer_task = generic; pa->pick_left_primer = 0; pa->pick_right_primer = 0; pa->pick_internal_oligo = 1; } else if (!strcmp_nocase(task_tmp, "generic")) { pa->primer_task = generic; } else if (!strcmp_nocase(task_tmp, "pick_detection_primers")) { pa->primer_task = generic; /* Deliberate duplication for backward compatibility. */ } else if (!strcmp_nocase(task_tmp, "pick_cloning_primers")) { pa->primer_task = pick_cloning_primers; } else if (!strcmp_nocase(task_tmp, "pick_discriminative_primers")) { pa->primer_task = pick_discriminative_primers; } else if (!strcmp_nocase(task_tmp, "pick_sequencing_primers")) { pa->primer_task = pick_sequencing_primers; } else if (!strcmp_nocase(task_tmp, "pick_primer_list")) { pa->primer_task = pick_primer_list; } else if (!strcmp_nocase(task_tmp, "check_primers")) { pa->primer_task = check_primers; /* check_primers sets the picking flags itself */ pa->pick_left_primer = 0; pa->pick_right_primer = 0; pa->pick_internal_oligo = 0; if (sa->left_input){ pa->pick_left_primer = 1; } if (sa->right_input){ pa->pick_right_primer = 1; } if (sa->internal_input){ pa->pick_internal_oligo = 1; } } else pr_append_new_chunk(glob_err, "Unrecognized PRIMER_TASK"); free(task_tmp); } /* * WARNING: read_and_create_seq_lib uses p3_read_line, so repeat * library files cannot be read inside the * while ((s = p3_read_line(stdin))...) loop above. * * FIX ME, in fact the reading * of the library contents probably belongs inside * primer3_boulder_main.c or libprimer3.c. */ /* Read in the repeat libraries */ if (NULL != repeat_file_path) { destroy_seq_lib(pa->p_args.repeat_lib); if ('\0' == *repeat_file_path) { /* Input now specifies no repeat library. */ pa->p_args.repeat_lib = NULL; } else { pa->p_args.repeat_lib = read_and_create_seq_lib(repeat_file_path, "mispriming library"); if(pa->p_args.repeat_lib->error.data != NULL) { pr_append_new_chunk(glob_err, pa->p_args.repeat_lib->error.data); } } free(repeat_file_path); repeat_file_path = NULL; } /* Read in the repeat libraries for internal oligo */ if (NULL != int_repeat_file_path) { destroy_seq_lib(pa->o_args.repeat_lib); if ('\0' == *int_repeat_file_path) { /* Input now specifies no mishybridization library. */ pa->o_args.repeat_lib = NULL; } else { pa->o_args.repeat_lib = read_and_create_seq_lib(int_repeat_file_path, "internal oligo mishyb library"); if(pa->o_args.repeat_lib->error.data != NULL) { pr_append_new_chunk(glob_err, pa->o_args.repeat_lib->error.data); } } free(int_repeat_file_path); int_repeat_file_path = NULL; } if (pa->primer_task == pick_pcr_primers_and_hyb_probe) { PR_ASSERT(pa->pick_internal_oligo); } /* Removed 10/20/2010 as excessively compulsive, especially in the context of taking input from web pages. if ((min_3_prime || min_5_prime) && (sa->primer_overlap_junctions_count == 0)) { pr_append_new_chunk(warnings, "SEQUENCE_OVERLAP_JUNCTION_LIST not given, but " "PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION or " "PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION specified"); } */ return 1; } #undef COMPARE #undef COMPARE_AND_MALLOC #undef COMPARE_INT #undef COMPARE_FLOAT #undef COMPARE_INTERVAL_LIST static void pr_append2(pr_append_str *err, const char* s1, const char* s2) { pr_append_new_chunk(err, s1); pr_append(err, s2); } int read_p3_file(const char *file_name, const p3_file_type expected_file_type, int echo_output, int strict_tags, p3_global_settings *pa, seq_args *sa, pr_append_str *fatal_err, pr_append_str *nonfatal_err, pr_append_str *warnings, read_boulder_record_results *read_boulder_record_res) { FILE *file; int ret_par = 0; int io_version = 4; char *line1; char *line2; char *line3; p3_file_type file_type = all_parameters; /* Check if a file name was provided */ PR_ASSERT(NULL != file_name); /* Open the file */ if (!(file = fopen(file_name,"r"))) { pr_append2(fatal_err, "Cannot open ", file_name); return ret_par; } /* Parse and interpret the first 3 lines */ /* Line 1 */ line1 = p3_read_line(file); if (!line1) { pr_append2(fatal_err, "Settings file is empty: ", file_name); return ret_par; } if (strcmp(line1, "Primer3 File - http://primer3.sourceforge.net")) { pr_append2(fatal_err, "First line must be \"Primer3 File - http://primer3.sourceforge.net\" in ", file_name); return ret_par; } /* Line 2 */ line2 = p3_read_line(file); if (!line2) { pr_append2(fatal_err, "Incorrect file format (too few lines) in ", file_name); return ret_par; } if (!strcmp(line2,"P3_FILE_TYPE=all_parameters")) { file_type = all_parameters; } else if (!strcmp(line2,"P3_FILE_TYPE=sequence")) { file_type = sequence; } else if (!strcmp(line2,"P3_FILE_TYPE=settings")) { file_type = settings; } else { pr_append2(fatal_err, "Unknown file type in at line 2 (line2='", line2); pr_append(fatal_err, "') in "); pr_append(fatal_err, file_name); return ret_par; } if (echo_output) { printf("P3_SETTINGS_FILE_USED=%s\n", file_name); printf("%s\n", line2); } /* Line 3 */ line3 = p3_read_line(file); if (!line3) { pr_append2(fatal_err, "Incorrect file format (too few lines) in ", file_name); return ret_par; } if (strcmp(line3, "")) { pr_append2(fatal_err, "Line 3 must be empty in ", file_name); return ret_par; } /* Check if the file type matches the expected type */ if (file_type != expected_file_type){ pr_append_new_chunk(nonfatal_err, "Unexpected P3 file type parsed"); } /* read the file */ ret_par = read_boulder_record(file, &strict_tags, &io_version, echo_output, expected_file_type, pa, sa, fatal_err, nonfatal_err, warnings, read_boulder_record_res); if (echo_output) printf("P3_SETTINGS_FILE_END=\n"); if (file) fclose(file); return ret_par; } static void tag_syntax_error(const char *tag_name, const char *datum, pr_append_str *err) { pr_append_new_chunk(err, "Illegal "); pr_append(err, tag_name); pr_append(err, " value: "); pr_append(err, datum); } static void parse_double(const char *tag_name, const char *datum, double *out, pr_append_str *err) { char *nptr; *out = strtod(datum, &nptr); if (nptr == datum) { /* Empty string or complete junk. */ tag_syntax_error(tag_name, datum, err); *out = 0.0; return; } /* Look for trailing junk. */ while (*nptr != '\n' && *nptr != '\0') { if (*nptr != ' ' && *nptr != '\t') { tag_syntax_error(tag_name, datum, err); break; } nptr++; } } static void parse_int(const char *tag_name, const char *datum, int *out, pr_append_str *err) { char *nptr; long tlong; tlong = strtol(datum, &nptr, 10); if (tlong > INT_MAX || tlong < INT_MIN) { tag_syntax_error(tag_name, datum, err); pr_append(err, " (value too large or too small)"); return; } *out = tlong; if (nptr == datum) { /* Empty string or complete junk. */ tag_syntax_error(tag_name, datum, err); return; } /* Look for trailing junk. */ while (*nptr != '\n' && *nptr != '\0') { if (*nptr != ' ' && *nptr != '\t') { tag_syntax_error(tag_name, datum, err); break; } nptr++; } } /* * For correct input, return a pointer to the first non-tab, non-space * character after the second integer, and place the integers in out1 and * out2. On incorrect input, return NULL; */ static const char * parse_int_pair(const char *tag_name, const char *datum, char sep, /* The separator, e.g. ',' or '-'. */ int *out1, int *out2, /* The 2 integers. */ pr_append_str *err) /* Error messages. */ { char *nptr, *tmp; long tlong; tlong = strtol(datum, &nptr, 10); if (tlong > INT_MAX || tlong < INT_MIN) { tag_syntax_error(tag_name, datum, err); pr_append(err, " (value too large or too small)"); return NULL; } *out1 = tlong; if (nptr == datum) { tag_syntax_error(tag_name, datum, err); return NULL; } while (' ' == *nptr || '\t' == *nptr) nptr++; if (sep != *nptr) { tag_syntax_error(tag_name, datum, err); return NULL; } nptr++; /* Advance past separator. */ while (' ' == *nptr || '\t' == *nptr) nptr++; tmp = nptr; tlong = strtol(tmp, &nptr, 10); if (tlong > INT_MAX || tlong < INT_MIN) { tag_syntax_error(tag_name, datum, err); pr_append(err, " (value too large or too small)"); return NULL; } *out2 = tlong; if (nptr == tmp) { tag_syntax_error(tag_name, datum, err); return NULL; } while (' ' == *nptr || '\t' == *nptr) nptr++; /* A hack to live with the old TARGET syntax. */ if (',' == *nptr && !strcmp(tag_name, "TARGET")) { /* Skip the old-fashioned "description". */ while(' ' != *nptr && '\t' != *nptr && '\0' != *nptr && '\n' != *nptr) nptr++; /* Advance to non-space, non-tab. */ while (' ' == *nptr || '\t' == *nptr) nptr++; } return nptr; } static void parse_interval_list(const char *tag_name, const char *datum, interval_array_t2 *interval_arr, pr_append_str *err) { const char *p = datum; int i1, i2; int ret = 0; while (' ' == *p || '\t' == *p) p++; while (*p != '\0' && *p != '\n') { p = parse_int_pair(tag_name, p, ',', &i1, &i2, err); if (NULL == p) return; ret = p3_add_to_interval_array(interval_arr, i1, i2); if (ret) { pr_append_new_chunk(err, "Too many elements for tag "); pr_append(err, tag_name); return; } } } /* * For correct input, return a pointer to the first non-tab, non-space * character after the forth integer and after the separator sep2, and * place the integers in out1, out2, out3 and out4. On incorrect input, * return NULL; If any of the 4 integers is not specified, the * corresponding output value will be -1. */ static char * parse_2_int_pair(const char *tag_name, char *datum, char sep, /* The separator between 2 numbers, e.g. ',' or '-'. */ char sep2, /* Separator between 2 intervals */ int *out1, int *out2, int *out3, int *out4, /* The 4 integers. */ pr_append_str *err) /* Error messages. */ { char *nptr, *tmp; long tlong; nptr = datum; if (*nptr == sep) { *out1 = -1; } else { tlong = strtol(datum, &nptr, 10); if (tlong > INT_MAX || tlong < INT_MIN) { tag_syntax_error(tag_name, datum, err); pr_append(err, " (value too large or too small)"); return NULL; } *out1 = tlong; if (nptr == datum) { tag_syntax_error(tag_name, datum, err); return NULL; } } while (' ' == *nptr || '\t' == *nptr) nptr++; if (sep != *nptr) { tag_syntax_error(tag_name, datum, err); return NULL; } nptr++; /* Advance past separator. */ while (' ' == *nptr || '\t' == *nptr) nptr++; tmp = nptr; if (*nptr == sep) { *out2 = -1; } else { tlong = strtol(tmp, &nptr, 10); if (tlong > INT_MAX || tlong < INT_MIN) { tag_syntax_error(tag_name, datum, err); pr_append(err, " (value too large or too small)"); return NULL; } *out2 = tlong; if (nptr == tmp) { tag_syntax_error(tag_name, datum, err); return NULL; } } while (' ' == *nptr || '\t' == *nptr) nptr++; if (sep != *nptr) { tag_syntax_error(tag_name, datum, err); return NULL; } nptr++; /* Advance past separator. */ while (' ' == *nptr || '\t' == *nptr) nptr++; tmp = nptr; if (*nptr == sep) { *out3 = -1; } else { tlong = strtol(tmp, &nptr, 10); if (tlong > INT_MAX || tlong < INT_MIN) { tag_syntax_error(tag_name, datum, err); pr_append(err, " (value too large or too small)"); return NULL; } *out3 = tlong; if (nptr == tmp) { tag_syntax_error(tag_name, datum, err); return NULL; } } while (' ' == *nptr || '\t' == *nptr) nptr++; if (sep != *nptr) { tag_syntax_error(tag_name, datum, err); return NULL; } nptr++; /* Advance past separator. */ while (' ' == *nptr || '\t' == *nptr) nptr++; tmp = nptr; if ((*nptr == '\n') || (*nptr == '\0') || (*nptr == sep2)) { *out4 = -1; } else { tlong = strtol(tmp, &nptr, 10); if (tlong > INT_MAX || tlong < INT_MIN) { tag_syntax_error(tag_name, datum, err); pr_append(err, " (value too large or too small)"); return NULL; } *out4 = tlong; if (nptr == tmp) { tag_syntax_error(tag_name, datum, err); return NULL; } } while (' ' == *nptr || '\t' == *nptr) nptr++; /* Should have reached an interval separator or end of line/string */ if ((*nptr != sep2) && (*nptr != '\0') && (*nptr != '\n')) { tag_syntax_error(tag_name, datum, err); return NULL; } if (*nptr == sep2) { /* Advance past the interval separator */ nptr++; } while (' ' == *nptr || '\t' == *nptr) nptr++; return nptr; } static void parse_2_interval_list(const char *tag_name, char *datum, interval_array_t4 *interval_arr, pr_append_str *err) { char *p = datum; int i1, i2, i3, i4; int ret = 0; interval_arr->count = 0; interval_arr->any_pair = 0; interval_arr->any_left = interval_arr->any_right = 0; while (' ' == *p || '\t' == *p) p++; while (*p != '\0' && *p != '\n') { p = parse_2_int_pair(tag_name, p, ',', ';', &i1, &i2, &i3, &i4, err); if (NULL == p) return; ret = p3_add_to_2_interval_array(interval_arr, i1, i2, i3, i4); if (ret == 1) { pr_append_new_chunk(err, "Too many elements for tag "); pr_append(err, tag_name); return; } else if (ret == 2) { pr_append_new_chunk(err, "Invalid range at tag "); pr_append(err, tag_name); return; } } } static int parse_intron_list(char *s, int *list, int *count) { long t; char *p, *q; *count = 0; p = q = s; while (*q != '\0' && *q != '\n') { t = strtol(p, &q, 10); if (q == p) { while (*q != '\0') { if (!isspace(*q)) { *count = 0; return 0; } q++; } return *count; } if (t > INT_MAX || t < INT_MIN) { return 0; } list[*count] = t; (*count)++; p = q; } return *count; } static void parse_product_size(const char *tag_name, char *in, p3_global_settings *pa, pr_append_str *err) { char *q, *s = in; const char *p; int i; /* * Handle possible double quotes around the value. * (This handling is needed for backward compatibility with v2.) */ if ('"' == *s) { s++; in++; q = strchr(s, '"'); if (NULL == q) { pr_append_new_chunk(err, tag_name); pr_append(err, " begins but does not end with a quote"); return; } /* Ignore the " and everything after it. */ *q = '\0'; } p = in; while (' ' == *p || '\t' == *p) p++; i = 0; while (*p != '\0' && *p != '\n') { if (i >= PR_MAX_INTERVAL_ARRAY) { pr_append_new_chunk(err, "Too many values for "); pr_append(err, tag_name); return; } p = parse_int_pair(tag_name, p, '-', &pa->pr_min[i], &pa->pr_max[i], err); if (NULL == p) return; i++; } pa->num_intervals = i; } /* Would be worthwhile to test this a bit more off-by-one errors on length of the quality score */ /* This function returns the number of elements in the sequence quality vector, and updates sargs->quality, sargs->n_quality, and sargs->quality_storage_size */ static int parse_seq_quality(char *s, seq_args *sargs) { long t; char *p, *q; p3_set_sa_empty_quality(sargs); p = q = s; while (*q != '\0' && *q != '\n') { t = strtol(p, &q, 10); if (q == p) { while (*q != '\0') { if (!isspace(*q)) { p3_set_sa_empty_quality(sargs); return 0; } q++; } return sargs->n_quality; } p3_sa_add_to_quality_array(sargs, t); p = q; } return sargs->n_quality; } /* =========================================================== */ /* Fail-stop wrapper for memory allocation. */ /* =========================================================== */ /* * Panic messages for when the program runs out of memory. */ static void * _rb_safe_malloc(size_t x) { void *r = malloc(x); if (NULL == r) out_of_memory_error(); return r; } static void pr_append(pr_append_str *x, const char *s) { if (pr_append_external(x, s)) out_of_memory_error(); } static void pr_append_new_chunk(pr_append_str *x, const char *s) { if (pr_append_new_chunk_external(x, s)) out_of_memory_error(); } static void out_of_memory_error() { fprintf(stderr, "out of memory in read_boulder\n"); exit(-2); } /* End of fail-stop wrappers. */ /* =========================================================== */ primer3-2.3.6/src/print_boulder.c0000664000077300007730000005002012231156245015656 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of primer3 and the primer3 suite. Primer3 and the primer3 suite are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this file (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include /* strcpy */ #include "print_boulder.h" static const char *pr_program_name = "Program name is probably primer3_core"; static void print_all_explain(const p3_global_settings *, const seq_args *, const p3retval *, int io_version); /* Print the data for chosen primer pairs to stdout in "boulderio" format. */ void print_boulder(int io_version, const p3_global_settings *pa, const seq_args *sa, const p3retval *retval, int explain_flag) { /* The pointers to warning tag */ char *warning; /* A place to put a string containing all error messages */ pr_append_str *combined_retval_err = NULL; /* A small spacer; WARNING this is a fixed size buffer, but plenty bigger than log(2^64, 10), the longest character string that is needed for a 64 bit integer. */ char suffix [100]; /* Pointers for the primer set just printing */ primer_rec *fwd, *rev, *intl; /* Variables only used for Primer Lists */ int num_fwd, num_rev, num_int, num_pair, num_print; int print_fwd = 0; int print_rev = 0; int print_int = 0; /* Switches for printing this primer */ int go_fwd = 0; int go_rev = 0; int go_int = 0; /* The number of loop cycles */ int loop_max; /* That links to the included region */ int i, incl_s = sa->incl_s; /* This deals with the renaming of the internal oligo */ const char *new_oligo_name = "INTERNAL"; char *int_oligo = (char*) new_oligo_name; /* Check: are all pointers linked to something*/ PR_ASSERT(NULL != pa); PR_ASSERT(NULL != sa); /* Check if there are warnings and print them */ if ((warning = p3_get_rv_and_gs_warnings(retval, pa)) != NULL) { printf("PRIMER_WARNING=%s\n", warning); free(warning); } /* Check if a settings file was read an print its id * Not needed anymore, we do this when we read the setting file. */ /* if (pa->settings_file_id != NULL) { printf("P3_FILE_ID=%s\n", pa->settings_file_id); free(warning); } */ combined_retval_err = create_pr_append_str(); if (NULL == combined_retval_err) exit(-2); /* Out of memory */ if (pr_append_new_chunk_external(combined_retval_err, retval->glob_err.data)) exit(-2); if (pr_append_new_chunk_external(combined_retval_err, retval->per_sequence_err.data)) exit(-2); /* Check if there are errors, print and return */ if (!pr_is_empty(combined_retval_err)) { print_boulder_error(pr_append_str_chars(combined_retval_err)); destroy_pr_append_str(combined_retval_err); return; } destroy_pr_append_str(combined_retval_err); /* Get how many primers are in the array */ num_fwd = retval->fwd.num_elem; num_rev = retval->rev.num_elem; num_int = retval->intl.num_elem; num_pair = retval->best_pairs.num_pairs; /* Prints out statistics about the primers */ if (explain_flag) print_all_explain(pa, sa, retval, io_version); /* Print out the stop codon if a reading frame was specified */ if (!PR_START_CODON_POS_IS_NULL(sa)) printf("PRIMER_STOP_CODON_POSITION=%d\n", retval->stop_codon_pos); /* How often has the loop to be done? */ if (retval->output_type == primer_list) { /* For Primer Lists: Figure out how many primers are in * the array that can be printed. If more than needed, * set it to the number requested. */ /* Get how may primers should be printed */ num_print = pa->num_return; /* Set how many primers will be printed */ print_fwd = (num_print < num_fwd) ? num_print : num_fwd; print_rev = (num_print < num_rev) ? num_print : num_rev; print_int = (num_print < num_int) ? num_print : num_int; /* Get which list has to print most primers */ loop_max = 0; if (loop_max < print_fwd) { loop_max = print_fwd; } if (loop_max < print_rev) { loop_max = print_rev; } if (loop_max < print_int) { loop_max = print_int; } /* Now the vars are there how often we have to go * through the loop and how many of each primer can * be printed. */ num_pair = 0; } else { loop_max = num_pair; /* Set how many primers will be printed */ print_fwd = num_pair; print_rev = num_pair; if (num_int != 0) { print_int = num_pair; } } if (io_version == 4) { printf("PRIMER_LEFT_NUM_RETURNED=%d\n", print_fwd); printf("PRIMER_RIGHT_NUM_RETURNED=%d\n", print_rev); printf("PRIMER_%s_NUM_RETURNED=%d\n", int_oligo, print_int); printf("PRIMER_PAIR_NUM_RETURNED=%d\n", num_pair); } /* --------------------------------------- */ /* Start of the loop printing all pairs or primers or oligos */ for(i=0; ioutput_type == primer_list) { /* Attach the selected primers to the pointers */ fwd = &retval->fwd.oligo[i]; rev = &retval->rev.oligo[i]; intl = &retval->intl.oligo[i]; /* Do fwd oligos have to be printed? */ if ((pa->pick_left_primer) && (i < print_fwd)) { go_fwd = 1; } else { go_fwd = 0; } /* Do rev oligos have to be printed? */ if ((pa->pick_right_primer) && (i < print_rev)) { go_rev = 1; } else { go_rev = 0; } /* Do int oligos have to be printed? */ if ((pa->pick_internal_oligo) && (i < print_int)) { go_int = 1; } else { go_int = 0; } } else { /* We will print primer pairs or pairs plus internal oligos */ /* Get pointers to the primer_rec's that we will print */ fwd = retval->best_pairs.pairs[i].left; rev = retval->best_pairs.pairs[i].right; intl = retval->best_pairs.pairs[i].intl; /* Pairs must have fwd and rev primers */ go_fwd = 1; go_rev = 1; /* Do hyb oligos have to be printed? */ if (pa->pick_internal_oligo == 1) { go_int = 1; } else { go_int = 0; } } /* Get the number for pimer counting in suffix[0] */ sprintf(suffix, "_%d", i); /* Print out the Pair Penalties */ if (retval->output_type == primer_pairs) { printf("PRIMER_PAIR%s_PENALTY=%f\n", suffix, retval->best_pairs.pairs[i].pair_quality); } /* Print single primer penalty */ if (go_fwd == 1) printf("PRIMER_LEFT%s_PENALTY=%f\n", suffix, fwd->quality); if (go_rev == 1) printf("PRIMER_RIGHT%s_PENALTY=%f\n", suffix, rev->quality); if (go_int == 1) printf("PRIMER_%s%s_PENALTY=%f\n", int_oligo, suffix, intl->quality); /* Print the oligo_problems */ if (io_version == 4) { if (go_fwd == 1 && p3_ol_has_any_problem(fwd)) printf("PRIMER_LEFT%s_PROBLEMS=%s\n", suffix, p3_get_ol_problem_string(fwd)); if (go_rev == 1 && p3_ol_has_any_problem(rev)) printf("PRIMER_RIGHT%s_PROBLEMS=%s\n", suffix, p3_get_ol_problem_string(rev)); if (go_int == 1 && p3_ol_has_any_problem(intl)) printf("PRIMER_%s%s_PROBLEMS=%s\n", int_oligo, suffix, p3_get_ol_problem_string(intl)); } /* Print primer sequences. */ if (go_fwd == 1) printf("PRIMER_LEFT%s_SEQUENCE=%s\n", suffix, pr_oligo_sequence(sa, fwd)); if (go_rev == 1) printf("PRIMER_RIGHT%s_SEQUENCE=%s\n", suffix, pr_oligo_rev_c_sequence(sa, rev)); if(go_int == 1) printf("PRIMER_%s%s_SEQUENCE=%s\n", int_oligo, suffix, pr_oligo_sequence(sa,intl)); /* Print primer start and length */ if (go_fwd == 1) printf("PRIMER_LEFT%s=%d,%d\n", suffix, fwd->start + incl_s + pa->first_base_index, fwd->length); if (go_rev == 1) printf("PRIMER_RIGHT%s=%d,%d\n", suffix, rev->start + incl_s + pa->first_base_index, rev->length); if (go_int == 1) printf("PRIMER_%s%s=%d,%d\n", int_oligo, suffix, intl->start + incl_s + pa->first_base_index, intl->length); /* Print primer Tm */ if (go_fwd == 1) printf("PRIMER_LEFT%s_TM=%.3f\n", suffix, fwd->temp); if (go_rev == 1) printf("PRIMER_RIGHT%s_TM=%.3f\n", suffix, rev->temp); if (go_int == 1) printf("PRIMER_%s%s_TM=%.3f\n", int_oligo, suffix, intl->temp); /* Print primer GC content */ if (go_fwd == 1) printf("PRIMER_LEFT%s_GC_PERCENT=%.3f\n", suffix, fwd->gc_content); if (go_rev == 1) printf("PRIMER_RIGHT%s_GC_PERCENT=%.3f\n", suffix, rev->gc_content); if (go_int == 1) printf("PRIMER_%s%s_GC_PERCENT=%.3f\n", int_oligo, suffix, intl->gc_content); /* Print primer self_any */ if (go_fwd == 1 && pa->thermodynamic_oligo_alignment==0) printf("PRIMER_LEFT%s_SELF_ANY=%.2f\n", suffix, fwd->self_any); if (go_rev == 1 && pa->thermodynamic_oligo_alignment==0) printf("PRIMER_RIGHT%s_SELF_ANY=%.2f\n", suffix, rev->self_any); if (go_int == 1 && pa->thermodynamic_oligo_alignment==0) printf("PRIMER_%s%s_SELF_ANY=%.2f\n", int_oligo, suffix, intl->self_any); if (go_int == 1 && pa->thermodynamic_oligo_alignment==1) printf("PRIMER_%s%s_SELF_ANY_TH=%.2f\n", int_oligo, suffix, intl->self_any); /* Print primer self_any thermodynamical approach */ if (go_fwd == 1 && pa->thermodynamic_oligo_alignment==1) printf("PRIMER_LEFT%s_SELF_ANY_TH=%.2f\n", suffix, fwd->self_any); if (go_rev == 1 && pa->thermodynamic_oligo_alignment==1) printf("PRIMER_RIGHT%s_SELF_ANY_TH=%.2f\n", suffix, rev->self_any); /* Print primer self_end*/ if (go_fwd == 1 && pa->thermodynamic_oligo_alignment==0) printf("PRIMER_LEFT%s_SELF_END=%.2f\n", suffix, fwd->self_end); if (go_rev == 1 && pa->thermodynamic_oligo_alignment==0) printf("PRIMER_RIGHT%s_SELF_END=%.2f\n", suffix, rev->self_end); if (go_int == 1 && pa->thermodynamic_oligo_alignment==0) printf("PRIMER_%s%s_SELF_END=%.2f\n", int_oligo, suffix, intl->self_end); if (go_int == 1 && pa->thermodynamic_oligo_alignment==1) printf("PRIMER_%s%s_SELF_END_TH=%.2f\n", int_oligo, suffix, intl->self_end); /* Print primer self_end thermodynamical approach */ if (go_fwd == 1 && pa->thermodynamic_oligo_alignment==1) printf("PRIMER_LEFT%s_SELF_END_TH=%.2f\n", suffix, fwd->self_end); if (go_rev == 1 && pa->thermodynamic_oligo_alignment==1) printf("PRIMER_RIGHT%s_SELF_END_TH=%.2f\n", suffix, rev->self_end); /* Print primer hairpin */ if (go_fwd == 1 && pa->thermodynamic_oligo_alignment==1) printf("PRIMER_LEFT%s_HAIRPIN_TH=%.2f\n", suffix, fwd->hairpin_th); if (go_rev == 1 && pa->thermodynamic_oligo_alignment==1) printf("PRIMER_RIGHT%s_HAIRPIN_TH=%.2f\n", suffix, rev->hairpin_th); if (go_int == 1 && pa->thermodynamic_oligo_alignment==1) printf("PRIMER_%s%s_HAIRPIN_TH=%.2f\n", int_oligo, suffix, intl->hairpin_th); /*Print out primer mispriming scores */ if (seq_lib_num_seq(pa->p_args.repeat_lib) > 0) { if (go_fwd == 1) printf("PRIMER_LEFT%s_LIBRARY_MISPRIMING=%.2f, %s\n", suffix, fwd->repeat_sim.score[fwd->repeat_sim.max], fwd->repeat_sim.name); if (go_rev == 1) printf("PRIMER_RIGHT%s_LIBRARY_MISPRIMING=%.2f, %s\n", suffix, rev->repeat_sim.score[rev->repeat_sim.max], rev->repeat_sim.name); if (retval->output_type == primer_pairs) printf("PRIMER_PAIR%s_LIBRARY_MISPRIMING=%.2f, %s\n", suffix, retval->best_pairs.pairs[i].repeat_sim, retval->best_pairs.pairs[i].rep_name); } /* Print out internal oligo mispriming scores */ if (go_int == 1 && seq_lib_num_seq(pa->o_args.repeat_lib) > 0) printf("PRIMER_%s%s_LIBRARY_MISHYB=%.2f, %s\n", int_oligo, suffix, intl->repeat_sim.score[intl->repeat_sim.max], intl->repeat_sim.name); /* If a sequence quality was provided, print it*/ if (NULL != sa->quality){ if (go_fwd == 1) printf("PRIMER_LEFT%s_MIN_SEQ_QUALITY=%d\n", suffix, fwd->seq_quality); if (go_rev == 1) printf("PRIMER_RIGHT%s_MIN_SEQ_QUALITY=%d\n", suffix, rev->seq_quality); if (go_int == 1 && (retval->output_type == primer_list)) printf("PRIMER_%s%s_MIN_SEQ_QUALITY=%d\n", int_oligo, suffix, intl->seq_quality); /* Has to be here and in primer pairs for backward compatibility */ } /* Print position penalty, this is for backward compatibility */ if (!_PR_DEFAULT_POSITION_PENALTIES(pa) || !PR_START_CODON_POS_IS_NULL(sa)){ printf("PRIMER_LEFT%s_POSITION_PENALTY=%f\n", suffix, fwd->position_penalty); printf("PRIMER_RIGHT%s_POSITION_PENALTY=%f\n", suffix, rev->position_penalty); } /* Print primer end stability */ if (go_fwd == 1) printf("PRIMER_LEFT%s_END_STABILITY=%.4f\n", suffix, fwd->end_stability); if (go_rev == 1) printf("PRIMER_RIGHT%s_END_STABILITY=%.4f\n", suffix, rev->end_stability); /* Print primer template mispriming */ if ( (pa->thermodynamic_template_alignment == 0) && (go_fwd == 1) && (oligo_max_template_mispriming(fwd) != ALIGN_SCORE_UNDEF)) printf("PRIMER_LEFT%s_TEMPLATE_MISPRIMING=%.4f\n", suffix, oligo_max_template_mispriming(fwd)); if ( (pa->thermodynamic_template_alignment == 0) && (go_rev == 1) && (oligo_max_template_mispriming(rev) != ALIGN_SCORE_UNDEF)) printf("PRIMER_RIGHT%s_TEMPLATE_MISPRIMING=%.4f\n", suffix, oligo_max_template_mispriming(rev)); /* Print primer template mispriming, thermodynamical approach*/ if ( (pa->thermodynamic_template_alignment == 1) && (go_fwd == 1) && (oligo_max_template_mispriming_thermod(fwd) != ALIGN_SCORE_UNDEF)) { printf("PRIMER_LEFT%s_TEMPLATE_MISPRIMING_TH=%.4f\n", suffix, oligo_max_template_mispriming_thermod(fwd)); } if ( (pa->thermodynamic_template_alignment == 1) && (go_rev == 1) && (oligo_max_template_mispriming_thermod(rev) != ALIGN_SCORE_UNDEF)) { printf("PRIMER_RIGHT%s_TEMPLATE_MISPRIMING_TH=%.4f\n", suffix, oligo_max_template_mispriming_thermod(rev)); #if 0 printf("DEBUG_PRIMER_RIGHT%s_TEMPLATE_MISPRIMING_TOP_TH=%.4f\n", suffix, rev->template_mispriming); printf("DEBUG_PRIMER_RIGHT%s_TEMPLATE_MISPRIMING_R_TH=%.4f\n", suffix, rev->template_mispriming_r); #endif } /************************************************************************************/ /* Print the pair parameters*/ if (retval->output_type == primer_pairs) { if (go_int == 1 && NULL != sa->quality) /* FIX ME - Uptate the tests */ printf("PRIMER_%s%s_MIN_SEQ_QUALITY=%d\n", int_oligo, suffix, intl->seq_quality); /* Print pair comp_any */ if(pa->thermodynamic_oligo_alignment==0) printf("PRIMER_PAIR%s_COMPL_ANY=%.2f\n", suffix, retval->best_pairs.pairs[i].compl_any); if(pa->thermodynamic_oligo_alignment==1) printf("PRIMER_PAIR%s_COMPL_ANY_TH=%.2f\n", suffix, retval->best_pairs.pairs[i].compl_any); /* Print pair comp_end */ if(pa->thermodynamic_oligo_alignment==0) printf("PRIMER_PAIR%s_COMPL_END=%.2f\n", suffix, retval->best_pairs.pairs[i].compl_end); if(pa->thermodynamic_oligo_alignment==1) printf("PRIMER_PAIR%s_COMPL_END_TH=%.2f\n", suffix, retval->best_pairs.pairs[i].compl_end); /* Print product size */ printf("PRIMER_PAIR%s_PRODUCT_SIZE=%d\n", suffix, retval->best_pairs.pairs[i].product_size); /* Print the product Tm if a Tm range is defined */ if (pa->product_max_tm != PR_DEFAULT_PRODUCT_MAX_TM || pa->product_min_tm != PR_DEFAULT_PRODUCT_MIN_TM) { printf("PRIMER_PAIR%s_PRODUCT_TM=%.4f\n", suffix, retval->best_pairs.pairs[i].product_tm); printf("PRIMER_PAIR%s_PRODUCT_TM_OLIGO_TM_DIFF=%.4f\n", suffix, retval->best_pairs.pairs[i].product_tm_oligo_tm_diff); printf("PRIMER_PAIR%s_T_OPT_A=%.4f\n", suffix, retval->best_pairs.pairs[i].t_opt_a); } /* Print the primer pair template mispriming */ if ((pa->thermodynamic_template_alignment == 0) && (retval->best_pairs.pairs[i].template_mispriming != ALIGN_SCORE_UNDEF)) printf("PRIMER_PAIR%s_TEMPLATE_MISPRIMING=%.2f\n", suffix, retval->best_pairs.pairs[i].template_mispriming); /* Print the primer pair template mispriming. Thermodynamic approach. */ if ((pa->thermodynamic_template_alignment == 1) && (retval->best_pairs.pairs[i].template_mispriming != ALIGN_SCORE_UNDEF)) printf("PRIMER_PAIR%s_TEMPLATE_MISPRIMING_TH=%.2f\n", suffix, retval->best_pairs.pairs[i].template_mispriming); } /* End of print parameters of primer pairs */ } /* End of the big loop printing all data */ /* End the print with newline and flush all buffers */ printf("=\n"); if (fflush(stdout) == EOF) { perror("fflush(stdout) failed"); exit(-1); } } void print_boulder_error(const char *err) { printf("PRIMER_ERROR=%s\n=\n", err); if (fflush(stdout) == EOF) { perror("fflush(stdout) failed"); exit(-1); } } void print_boulder_warning(const char *err) { printf("PRIMER_WARNING=%s\n", err); if (fflush(stdout) == EOF) { perror("fflush(stdout) failed"); exit(-1); } } static void print_all_explain(const p3_global_settings *pa, const seq_args *sa, const p3retval *retval, int io_version) { if (pa->pick_left_primer == 1 && !(pa->pick_anyway && sa->left_input)) printf("PRIMER_LEFT_EXPLAIN=%s\n", p3_get_oligo_array_explain_string(p3_get_rv_fwd(retval))); if (pa->pick_right_primer == 1 && !(pa->pick_anyway && sa->right_input)) printf("PRIMER_RIGHT_EXPLAIN=%s\n", p3_get_oligo_array_explain_string(p3_get_rv_rev(retval))); if ( pa->pick_internal_oligo == 1 && !(pa->pick_anyway && sa->internal_input)) printf("PRIMER_INTERNAL_EXPLAIN=%s\n", p3_get_oligo_array_explain_string(p3_get_rv_intl(retval))); if (pa->pick_right_primer == 1 && pa->pick_left_primer == 1) { printf("PRIMER_PAIR_EXPLAIN=%s\n", p3_get_pair_array_explain_string(p3_get_rv_best_pairs(retval))); } } primer3-2.3.6/src/thal.h0000775000077300007730000001275212231156245013760 0ustar ioanaioana/* Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009,2010 2011,2012 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), and Helen Skaletsky All rights reserved. This file is part the primer3 software suite. This software suite is free software; you can redistribute is and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this file (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, ST RICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #ifndef _THAL_H #define _THAL_H #include /* ! mul ei ole float.h-d includes DBL_MAX */ #include #include #ifndef THAL_ERROR_SCORE # define THAL_ERROR_SCORE -_INFINITY #endif /* The maximum length of _one_ of the two sequences being aligned in a thermodynamic alignment. In other words, the length of one sequence must be <= THAL_MAX_ALIGN, but the other sequence can be longer. The rationale behind this value (60) is that this is the maxium reasonable length for nearest neighbor models. It is the maxium length at which we can restrict our model to only two states of melting: fully intact duplex or completely dissociated single strands. */ #ifndef THAL_MAX_ALIGN #define THAL_MAX_ALIGN 60 #endif /* The maxium length of the other sequence in a thermodynamic alignment. This value can be increased, though alignments against very long sequences will be quite slow. As of 2012-05-18, we only potentially see sequences longer this when checking for mispriming in the template ('max_template_mispriming') in libprimer3.c, which is really designed to find sites of ectopic primer very close (a few kilobases) from the location of the cadidate primer. */ #ifndef THAL_MAX_SEQ #define THAL_MAX_SEQ 10000 #endif /*** BEGIN CONSTANTS ***/ extern const double _INFINITY; extern const double ABSOLUTE_ZERO; extern const int MAX_LOOP; /* the maximum size of loop that can be calculated; for larger loops formula must be implemented */ extern const int MIN_LOOP; /*** END CONSTANTS ***/ /* BEGIN TYPEDEFs */ typedef enum thal_alignment_type { thal_any = 1, thal_end1 = 2, thal_end2 = 3, thal_hairpin = 4, } thal_alignment_type; /* Structure for passing arguments to THermodynamic ALignment calculation */ typedef struct { int debug; /* if non zero, print debugging info to stderr */ thal_alignment_type type; /* one of the 1 THAL_ANY, (by default) 2 THAL_END1, 3 THAL_END2, 4 THAL_HAIRPIN */ int maxLoop; /* maximum size of loop to consider; longer than 30 bp are not allowed */ double mv; /* concentration of monovalent cations */ double dv; /* concentration of divalent cations */ double dntp; /* concentration of dNTP-s */ double dna_conc; /* concentration of oligonucleotides */ double temp; /* temperature from which hairpin structures will be calculated */ int temponly; /* if non zero, print only temperature to stderr */ int dimer; /* if non zero, dimer structure is calculated */ } thal_args; /* Structure for receiving results from the thermodynamic alignment calculation */ typedef struct { char msg[255]; double temp; int align_end_1; int align_end_2; } thal_results; /*** END OF TYPEDEFS ***/ void set_thal_default_args(thal_args *a); void set_thal_oligo_default_args(thal_args *a); /* Read the thermodynamic values (parameters) from the parameter files in the directory specified by 'path'. Return 0 on success and -1 on error. The thermodynamic values are stored in multiple static variables. */ /* Here is an example of how this function is used in primer3_boulder_main.c: */ #if 0 if (get_thermodynamic_values(thermodynamic_params_path, &o)) { fprintf(stderr, "%s\n", o.msg); exit(-1); } #endif int get_thermodynamic_values(const char* path, thal_results *o); void destroy_thal_structures(); /* Central method for finding the best alignment. On error, o->temp is set to THAL_ERROR_SCORE and a message is put in o->msg. The error might be caused by ENOMEM. To determine this it is necessary to check errno. */ void thal(const unsigned char *oligo1, const unsigned char *oligo2, const thal_args* a, thal_results* o); #endif primer3-2.3.6/primer3web_v3_0_0_default_settings.txt0000664000077300007730000001020612231156245021367 0ustar ioanaioanaPrimer3 File - http://primer3.sourceforge.net P3_FILE_TYPE=settings PRIMER_FIRST_BASE_INDEX=1 PRIMER_TASK=generic P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers PRIMER_MIN_THREE_PRIME_DISTANCE=3 PRIMER_EXPLAIN_FLAG=1 # Put something reasonable for PRIMER_MISPRIMING_LIBRARY depending # on the species you are designing primers for. PRIMER_MISPRIMING_LIBRARY= PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00 PRIMER_PRODUCT_SIZE_RANGE=150-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 # PRIMER_MAX_SELF_ANY_TH=45.00 PRIMER_MAX_SELF_END_TH=35.00 PRIMER_PAIR_MAX_COMPL_ANY_TH=45.00 PRIMER_PAIR_MAX_COMPL_END_TH=35.00 PRIMER_MAX_HAIRPIN_TH=24.00 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=40.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=70.00 PRIMER_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_MAX_SIZE=23 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=59.0 PRIMER_MAX_TM=62.0 PRIMER_PAIR_MAX_DIFF_TM=5.0 # PRIMER_TM_FORMULA=1 means use the SantaLucia parameters (Proc Natl Acad Sci 95:1460-65) PRIMER_TM_FORMULA=1 PRIMER_SALT_MONOVALENT=50.0 # PRIMER_SALT_CORRECTIONS=1 means use the salt correction in SantaLucia et al 1998 PRIMER_SALT_CORRECTIONS=1 # Millimolar conc of MgCl+2 PRIMER_SALT_DIVALENT=1.5 # Millimolar conc of dNTPs PRIMER_DNTP_CONC=0.6 PRIMER_DNA_CONC=50.0 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_LOWERCASE_MASKING=0 # PRIMER_MIN_GC=30.0 PRIMER_MAX_GC=70.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_POLY_X=4 PRIMER_OUTSIDE_PENALTY=0 PRIMER_GC_CLAMP=0 PRIMER_LIBERAL_BASE=1 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_PICK_ANYWAY=1 PRIMER_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_WT_SELF_END_TH=0.0 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_WT_NUM_NS=0.0 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_WT_END_QUAL=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_END_TH=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_END_QUAL=0.0 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_PRODUCT_MAX_TM=1000000.0 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_SEQUENCING_ACCURACY=20 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MAX_SELF_END=3.00 PRIMER_QUALITY_RANGE_MAX=100 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_SEQUENCING_INTERVAL=250 PRIMER_INTERNAL_SALT_DIVALENT=1.5 PRIMER_MAX_SELF_ANY=8.00 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_SEQUENCING_SPACING=500 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_MIN_END_QUALITY=0 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_SEQUENCING_LEAD=50 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_MAX_END_GC=5 PRIMER_MIN_QUALITY=0 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_WT_SELF_ANY=0.0 PRIMER_MAX_TEMPLATE_MISPRIMING=12.00 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_SELF_END=0.0 PRIMER_PRODUCT_OPT_SIZE=0 PRIMER_PRODUCT_OPT_TM=0.0 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=24.00 PRIMER_INSIDE_PENALTY=-1.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_PRODUCT_MIN_TM=-1000000.0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_WT_SELF_END=0.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_TM_LT=1.0 = primer3-2.3.6/primer3_manual.htm0000664000077300007730000061421712231156530015516 0ustar ioanaioana primer3 release 2.3.6 - manual

PRIMER3 RELEASE 2.3.6 MANUAL

CONTENTS

1. COPYRIGHT AND LICENSE
2. INTRODUCTION
3. CITING PRIMER3
4. FAIR USE OF PRIMER3
5. CHANGES FROM VERSION 2.2.3
6. INSTALLATION INSTRUCTIONS - UNIX/LINUX
7. INSTALLATION INSTRUCTIONS - Mac OSX
8. INSTALLATION INSTRUCTIONS - WINDOWS
9. BUILDING A Mac OSX UNIVERSAL BINARY
10. SYSTEM REQUIREMENTS
11. INVOKING primer3_core
12. COMMAND LINE ARGUMENTS
13. INPUT AND OUTPUT CONVENTIONS
14. AN EXAMPLE
15. HOW TO MIGRATE TAGS TO IO VERSION 4
16. "SEQUENCE" INPUT TAGS
17. "GLOBAL" INPUT TAGS
18. "PROGRAM" INPUT TAGS
19. HOW PRIMER3 CALCULATES THE PENALTY VALUE
20. PRIMER3 SETTINGS FILE FORMAT
21. OUTPUT TAGS
22. EXAMPLE OUTPUT
23. ADVICE FOR PICKING PRIMERS
24. CAUTIONS
25. WHAT TO DO IF PRIMER3 CANNOT FIND ANY PRIMERS?
26. DIFFERENCES FROM EARLIER VERSIONS
27. EXIT STATUS CODES
28. PRIMER3 WWW INTERFACES
29. ACKNOWLEDGMENTS

1. COPYRIGHT AND LICENSE

Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009,2010
              2011,2012,2013
Whitehead Institute for Biomedical Research, Steve Rozen
(http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky. All rights
reserved.

    This file is part of the primer3 suite and libraries.

    The primer3 suite and libraries are free software;
    you can redistribute them and/or modify them under the terms
    of the GNU General Public License as published by the Free
    Software Foundation; either version 2 of the License, or (at
    your option) any later version.

    This software is distributed in the hope that it will be useful,
    but WITHOUT ANY WARRANTY; without even the implied warranty of
    MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
    GNU General Public License for more details.

    You should have received a copy of the GNU General Public License
    along with this software (file gpl-2.0.txt in the source
    distribution); if not, write to the Free Software
    Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA

THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS
"AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR
A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT
OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL,
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2. INTRODUCTION

Primer3 picks primers for PCR reactions, considering as criteria:
o oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,

o PCR product size,

o positional constraints within the source (template) sequence, and

o possibilities for ectopic priming (amplifying the wrong sequence)

o many other constraints.

All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.

3. CITING PRIMER3

We request but do not require that use of this software be cited in publications as

Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M and Rozen SG.
Primer3--new capabilities and interfaces.
Nucleic Acids Res. 2012 Aug 1;40(15):e115.

The paper is available at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424584/

Source code available at http://sourceforge.net/projects/primer3/.

4. FAIR USE OF PRIMER3

The development of primer3 is promoted by a small group of enthusiastic scientists mainly in their free time.They do not gain any financial profit with primer3.

There are two groups of primer3 users: end users, who run primer3 to pick their primers and programmers, who use primer3 in their scripts or software packages. We encourage both to use primer3.

If you are an end user, we request but do not require that use of this software be cited in publications as listed above under CITING PRIMER3.

If you are a programmer, you will see that primer3 is now distributed under the GNU General Public License, version 2 or (at your option) any later version of the License (GPL2). As we understand it, if you include parts of the primer3 source code in your source code or link to primer3 binary libraries in your executable, you have to release your software also under GPL2. If you only call primer3 from your software and interpret its output, you can use any license you want for your software. If you modify primer3 and then release your modified software, you have to release your modifications in source code under GPL2 as well.

We chose GPL2 because we wanted primer3 to evolve and for the improvements to find their way back into the main distribution. If you are programming a new web interface which runs primer3, please include in the about page of the tool the sentence "<your software name> uses primer3 version ...". Please consider releasing your software under GPL2 as well, especially if you do not want to maintain it in the future.

There is no need to ask us for permission to include primer3 in your tools.

5. CHANGES FROM VERSION 2.2.3

1. The tag PRIMER_THERMODYNAMIC_ALIGNMENT was replaced by two new tags:

- PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT which governs hairpin and oligo-oligo interactions. Therefore, whenever it is set to 1 (the default) thermodynamic alignments will be used for oligo-oligo interactions and hairpins.

- PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT which governs the oligo-template interactions and, when set to 1 (default is 0), will cause primer3 to use the thermodynamic alignment against templates.

The reason for this change is to provide the option of using the old alignment for oligo-template interactions when the thermodynamic alignment is too slow or the template is too long (there is a hard limit THAL_MAX_SEQ=10k on the length of sequences involved in thermodynamic alignments).

2. We changed the following default values:

2.1. Changing default oligo temperature calculations

PRIMER_TM_FORMULA=1 (was 0)
PRIMER_SALT_CORRECTIONS=1 (was 0)

2.2. Making thermodynamic secondary structure calculations for oligos the default:

PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 (was 0)

2.3. The following need reasonable values to support the changes in 2.1 and 2.2 above:

PRIMER_SALT_DIVALENT=1.5 (was 0.0)
PRIMER_DNTP_CONC=0.6 (was 0.0)

2.4. To make old defaults easily accessible, we added a command line argument, --default_version=1, which directs primer3_core to use the old defaults. --default_version=2 directs primer3_core to use the new defaults. The default is --default_version=2.

2.5. IMPORTANT: because PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1, PRIMER_THERMODYNAMIC_PARAMETERS_PATH must point to the right location. This tag specifies the path to the directory that contains all the parameter files used by the thermodynamic approach. In Linux, there are two *default* locations that are tested if this tag is not defined: ./primer3_config/ and /opt/primer3_config/. For Windows, there is only one default location: .\primer3_config\. If the the parameter files are not in one these locations, be sure to set PRIMER_THERMODYNAMIC_PARAMETERS_PATH.

2.6. Changed default for

PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 (was 1)

(0 is almost always the the behavior one wants.)

2.7. To get the behavior of -default_version=1 when -default_version=2 set the following:

PRIMER_TM_FORMULA=0
PRIMER_SALT_CORRECTIONS=0
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=0
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0
PRIMER_SALT_DIVALENT=0.0
PRIMER_DNTP_CONC=0.0
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1

2.8. To get the behavior of -default_version=2 when -default_version=1 set the following:

PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0
PRIMER_SALT_DIVALENT=1.5
PRIMER_DNTP_CONC=0.6
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0

3. We changed the NULL value for SEQUENCE_FORCE_{LEFT,RIGHT}_START_END to -1000000, and made it an error to select PRIMER_FIRST_BASE_INDEX <= this value. This is to correct an error when SEQUENCE_FORCE_{LEFT,RIGHT}_START_END was -1 (value previously used to indicate a NULL) but PRIMER_FIRST_BASE_INDEX was < 0, which caused the intended NULL value (-1) to be treated as a constraint on primer location (a constraint that was not possible to satisfy).

4. We changed the PRIMER_TASK called 'pick_detection_primers' to 'generic' while retaining 'pick_detection_primers' as an alias for backward compatibility.

5. The code now uses 'end' alignments when assessing template mispriming using thermodynamic alignments. This is consistent with the approach taken with the previous alignment algorithm and with checking for mispriming against repeat libraries.

6. We removed PRIMER_PAIR_MAX_HAIRPIN_TH (which was ignored previously).

7. Primer3 now requires the user to set SEQUENCE_TARGET, not SEQUENCE_INCLUDED_REGION when PRIMER_TASK=pick_discriminative_primers

8. When PRIMER_TASK=pick_discriminative_primers or PRIMER_TASK=pick_cloning_primers the value of SEQUENCE_INCLUDED_REGION is no longer changed to the entire input sequence.

9. The handling of divalent cations when PRIMER_SALT_CORRECTIONS=2 (not the default and not the recommended value) has been updated. The rationale is that, when divalent cations are present, the formula by Owczarzy et al., 2004 (used previously by Primer3) can be improved upon as described in (Ahsen et al., 2010; Owczarzy et al., 2008). Therefore we have updated the melting temperature calculation to follow the scheme in Figure 9 of (Owczarzy et al., 2008). Please find references to these papers below in the manual.

10. We modified the error handling of non-memory related errors that can occur during the thermodynamic alignment. They are no longer fatal errors.

11. We removed io_version 3, which was only kept for backward compatibility with very old versions of primer3.

12. In addition, several error corrections:

12.1. Corrected a short, fixed-size buffer for the file/path names specified by the command line arguments -output, -error and for the settings file.

12.2. Two other corrected errors were in p3_set_gs_primer_self_end and p3_set_gs_primer_internal_oligo_self_end, which erroneously multiplied their 'val' arguments by 100.

12.3. Primer3 now detects and handles the situation in which user-supplied primers (SEQUENCE_PRIMER and SEQUENCE_PRIMER_REVCOMP) have the left primer to the right of the right primer. Also issue a warning if user-supplied primers occur in more than one location in the template.

6. INSTALLATION INSTRUCTIONS - UNIX/LINUX

Unzip and untar the distribution. DO NOT do this on a PC if you are going to compile and/or test on a different operation system. Primer3_core will not compile and various tests will fail if pc newlines get inserted into the code and test files. Instead, move the distribution (primer3-<release>.tar.gz) to Unix/Linux, and then

$ unzip primer3-<release>.tar.gz
$ tar xvf primer3-<release>.tar
$ cd primer3-<release>/src

If you do not use gcc, then you will probably have to modify the makefile to use your (ANSI) C compiler with the compile and link flags it understands.

$ make all

# You should have created executables primer3_core, ntdpal,
# olgotm, and long_seq_tm_test

$ make test

# You should not see 'FAILED' during the tests.

If your perl command is not called perl (for example, if it is called perl5) you will have to modify the Makefile in the test/ directory.

ntdpal (NucleoTide Dynamic Programming ALignment) is a stand-alone program that provides primer3's alignment functionality (local, a.k.a. Smith-Waterman, global, a.k.a. Needleman-Wunsch, plus "half global"). It is provided strictly as is; for further documentation please see the code.

7. INSTALLATION INSTRUCTIONS - Mac OSX

How to install this software
============================

1. Double click on the .tar.gz file to extract the archive.

2. The binary files are located in the 'bin' [for 'binary'] folder

3. (Optional) To run the tests, cd to the new directory and then the test folder

4. (Optional) Within this folder run: a. 'perl -w p3test.pl'

5. (Optional) You should not see 'FAILED' during the tests.

6. (Optional) *NOTE*: If your perl command is not called perl (for example, if it is called perl5) you will have to modify the internals of the test scripts).

7. Copy the following files to a location of your choice:
a. bin/long_seq_tm_test
b. bin/ntdpal
c. bin/oligotm
d. bin/primer3_core

8. (Optional) Make sure this location is within your $PATH (see below)


Where to put the binary files
=============================

A good place to put these is within ~/bin/ (this means in your home folder, within a folder named `bin` [for 'binary']).

You can also just drag the 'bin' folder to a location within your home directory.

You can certainly also copy the files within 'bin' to /usr/local/bin (if you are an administrator) or another similar location.

You may need to adjust the permissions on the binaries if you get fancy.


Add the location to your $PATH
==============================

This is an optional step, but it will allow you to run primer3 in any directory on your machine as your user just by typing its name (primer3_core).

*** You should be very careful when altering your $PATH as things can go very wrong. See below for an alternate method. ***

If you added the binaries to /usr/local/bin, then you do not need to do this.

If you added the binaries to a local directory (let's say ~/bin/), do the following:

1. Edit your ~/.bash_profile. You can edit this on the command line (Terminal) with:

nano ~/.bash_profile

2. Add the following line if it is not present (replacing '~/bin' if you used another directory):

PATH=$PATH:~/bin/

3. If a PATH line *is* present, ensure you add a colon to the end of what is there and then the directory, so if you have something like:

a) PATH=$PATH:/usr/local/genome/bin:/sw/bin

make it look like:

b) PATH=$PATH:/usr/local/genome/bin:/sw/bin:~/bin

4. Quit and restart terminal for the changes to take effect.

If you don't add the location to your $PATH
===========================================

Assuming you don't want to modify your $PATH, you can still run the binaries. Let's assume you put the files in '~/bin/. You may run primer3_core by doing either of the following:

1. ~/bin/primer3_core < yourInputFile
2. /Users/<your username>/bin/primer3_core < yourInputFile

The first option is just a shortcut to the second.

8. INSTALLATION INSTRUCTIONS - WINDOWS

How to install this software
============================

1. Unzip the '.zip' file downloaded from SourceForge.net
2. You will create a primer3-<release> folder in the location where the file was unzipped
3. You may copy the files from the 'bin' directory of the primer3-<release> folder to a location of your choice. The exact same files are located within the 'src' folder so that the tests may be run (windows does not allow relative paths in shortcuts).

Running the tests
=================
You must install a perl distribution to run the windows tests.

We *strongly* recommend you install ActiveState perl (http://www.activestate.com/products/activeperl/) as this was used to test our primer3 builds, and it is known to work.

1. Click on 'Start > Run...'
2. Type 'cmd' into the space provided
3. Hit enter (or select 'OK')
4. Navigate to the location of the tests:

A. if you put it in
C:/Documents and Settings/YourName/primer3-<release>/test/,
you would type
'cd c:/Documents and Settings/YourName/primer3-<release>/test/'

B. you can also type 'cd ' (don't forget the space after cd) and drag the primer3-<release> folder onto the command-line window from windows explorer, this will fill in the location for you

5. On the command line, run 'mingw32-make TESTOPTS=--windows' in this directory. You can also run the tests individually, for example 'perl p3test.pl --windows'.
6. You should see [OK] for all of the tests.

Running the software
====================

To run the program, you must do so from the MS-DOS command-line. The intricacies of the DOS command line are beyond the scope of this document. Google for more information, if needed. Here is a quick summary:

1. Click on 'Start > Run...'
2. Type 'cmd' into the space provided
3. Hit enter (or select 'OK')
4. Navigate to the location of the binary:

A. if you put it in
C:/Documents and Settings/YourName/Temp,
you would type
'cd c:/Documents and Settings/YourName/Temp'

B. you can also type 'cd ' (don't forget the space after cd) and drag the primer3 folder onto the command-line window from windows explorer, this will fill in the location for you

5. Run the example file by typing:

primer3_core.exe < example

Other files may be run in a similar fashion. If your input filename is 'MyData.txt' you can run primer3 using this file (in the correct format; see README) with:

primer3_core.exe < MyData.txt

If your file is not in the folder containing primer3_core.exe, you could run the program from the primer3_core folder using:

primer3_core.exe < c:/someOtherFolder/someOtherFolder/MyData.txt

Finally, if you want to run the program without going to its folder, assuming primer3_core.exe is in c:/Temp, you could run:

c:/Temp/primer3_core.exe < c:/someOtherFolder/someOtherFolder/MyData.txt

9. BUILDING A Mac OSX UNIVERSAL BINARY

** To build a processor-native, NON-universal binary of primer3, the following is NOT necessary**.

The instructions above should be sufficient.

A pre-compiled, universal binary download for OSX is available from http://sourceforge.net/projects/primer3/ for the current release.

These instructions assume you want to build binaries compatible with *both* of the current processor architectures used by the Apple platform (i,e. the binaries will be run on both PPC and Intel platforms).

Provided you have the OS X developer tools installed (you can download from http://developer.apple.com after registering for a free account), you can compile a universal build (Intel and PPC native) of primer3.

o you must be running OS X > 10.4 and should have the most recent version of XCode
o run `make -f Makefile.OSX.Leopard all` if you run OS X 10.5
o run `make -f Makefile.OSX.Tiger all` is you run OS X 10.4
o run the tests as directed above

Additional instructions for 'installing' the binaries may be found in the README.OSX.txt.

You should be able to compile a 3-way binary which includes PPC64 support (Intel, PPC, PPC64) by adding the `-arch ppc64` flag to the end of both the CFLAGS and LDFLAGS lines at the top of Makefile.OSX. This has not been tested.

10. SYSTEM REQUIREMENTS

Please see http://sourceforge.net/projects/primer3/ for up-to-date information. Primer3 should compile on any system using with an ANSI C compiler like Linux/Unix, MacOS 10 or MS Windows. The Makefile will probably need to be modified for compilation with C compilers other than gcc.

11. INVOKING primer3_core

By default, the executable program produced by the Makefile is called primer3_core. This is the C program that does the heavy lifting of primer picking. There are also two, more user-friendly, web interfaces (distributed separately).

The command line for primer3 is:

primer3_core [ -format_output ] [ -default_version=1|-default_version=2 ] [ -io_version=4 ] [ -p3_settings_file=<file_path> ] [ -echo_settings_file ] [ -strict_tags ] [ -output=<file_path> ] [ -error=<file_path> ] [ input_file ]

A complete list of valid command line arguments can be found in COMMAND LINE ARGUMENTS below.

If no input file is specified, primer3_core will read its input from stdin.

12. COMMAND LINE ARGUMENTS

This section presents the list of arguments that may given in the command line. Any unique abbreviation of the arguments is allowed (e.g. -ab instead of -about) and each argument can be preceded by either one or two dashes. In the case of arguments that receive values, the '=' can be replaced by a space.

-about

With this argument primer3 generates one line of output indicating the release number and then exits. This allows scripts to query primer3 for its version.

-default_version=n

n=2 is the default, and directs primer3_core to use the latest default values (the ones documented here). n=1 directs primer3_core to use defaults from version 2.2.3 and before.

-format_output

This argument indicates that primer3_core should generate user-oriented (rather than program-oriented) output.

-strict_tags

This argument indicates that primer3_core should generate a fatal error if there is any tag in the input that it does not recognize. This tag also applies to the settings file (see documentation for the -p3_settings_file argument), if any, but with limitations: lines that do not begin with PRIMER_ or P3_FILE_ID are always ignored in the settings file.

-p3_settings_file=file_path

This argument specifies a settings file, file_path. Primer3 reads the global ("PRIMER_...") parameters from this file first. Tags appearing in the settings file override default primer3 settings. The values set in the settings files can be also overridden by tags in the input file. See primer3 file documentation for details on the file format. WARNING: the -strict_tags flag applies only to tags beginning with the string "PRIMER_"; lines that do not begin with the string "PRIMER_" are ignored.

-echo_settings_file

This argument indicates that primer3_core should print the input tags specified in the given settings file. If no settings file is provided or if the -format_output option is given, this argument will have no effect.

-io_version=n

This argument is provided for backward compatibility. -io_version=4 is the only legal value and the default. -output=file_path This argument specifies the file where the output should be written. If omitted, stdout is used.

-error=file_path

This argument specifies the file where the error messages should be written. If omitted, stderr is used.

13. INPUT AND OUTPUT CONVENTIONS

By default, primer3 accepts input in Boulder-IO format, a pre-XML, pre-RDF, text-based input/output format for program-to-program data interchange. By default, primer3 also produces output in the same format.

When run with the -format_output command-line flag, primer3 prints a more user-oriented report for each sequence.

Primer3 exits with 0 status if it operates correctly. See EXIT STATUS CODES below for additional information.

The syntax of the version of Boulder-IO recognized by primer3 is as follows:

  o Input consists of a sequence of RECORDs.

  o A RECORD consists of a sequence of (TAG,VALUE) pairs, each terminated
    by a newline character (\n). A RECORD is terminated by  '='
    appearing by itself on a line.

  o A (TAG,VALUE) pair has the following requirements:

    o the TAG must be immediately (without spaces) 
          followed by '='.

An example of a legal (TAG,VALUE) pair is

SEQUENCE_ID=my_marker

and an example of a Boulder-IO record is

SEQUENCE_ID=test1
SEQUENCE_TEMPLATE=GACTGATCGATGCTAGCTACGATCGATCGATGCATGCTAGCTAGCTAGCTGCTAGC
=

Many records can be sent, one after another. Below is an example of three different records which might be passed through a Boulder-IO stream:

SEQUENCE_ID=test1
SEQUENCE_TEMPLATE=GACTGATCGATGCTAGCTACGATCGATCGATGCATGCTAGCTAGCTAGCTGCTAGC
=
SEQUENCE_ID=test2
SEQUENCE_TEMPLATE=CATCATCATCATCGATGCTAGCATCNNACGTACGANCANATGCATCGATCGT
=
SEQUENCE_ID=test3
SEQUENCE_TEMPLATE=NACGTAGCTAGCATGCACNACTCGACNACGATGCACNACAGCTGCATCGATGC
=

Primer3 reads Boulder-IO on stdin and echos its input and returns results in Boulder-IO format on stdout. Primer3 indicates many user-correctable errors by a value in the output tag PRIMER_ERROR (see below). Primer3 indicates other errors, including system configuration errors, resource errors (such out-of-memory errors), and detected programming errors by a message on stderr and a non-zero exit status.

This document lists the input tags that primer3 recognizes. Primer3 echos and ignores any tags it does not recognize, unless the -strict_tags flag is set on the command line, in which case primer3 prints an error in the PRIMER_ERROR output tag (see below), and prints additional information on stdout; this option can be useful for debugging systems that incorporate primer3.

Except for tags with the type "interval list" each tag should only appear ONCE in any given input record. This restriction is not systematically checked, and if a tag appears more than once, the new value silently over-writes the previous value.

14. AN EXAMPLE

One might be interested in performing PCR on an STS with a CA repeat in the middle of it. Primers need to be chosen based on the criteria of the experiment.

We need to create a Boulder-IO record to send to primer3 via stdin. There are lots of ways to accomplish this. We could save the record into a text file called 'example', and then type the Unix command 'primer3_core < example'.

Let's look at the input record itself:
SEQUENCE_ID=example
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_TASK=generic
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=75-100
P3_FILE_FLAG=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
PRIMER_EXPLAIN_FLAG=1
=

A breakdown of the reasoning behind each of the TAG=VALUE pairs is below:

SEQUENCE_ID=example

The main intent of this tag is to provide an identifier for the sequence that is meaningful to the user, for example when primer3 processes multiple records, and by default this tag is optional. However, this tag is _required_ when P3_FILE_FLAG is non-0 Because it provides the names of the files that contain lists of oligos that primer3 considered.

SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG

The SEQUENCE_TEMPLATE provides the sequence from which primer3 will design primers. Note that there is no newline until the sequence terminates completely.

SEQUENCE_TARGET=37,21

There is a simple sequence repeat in this sequence. The repeat starts at base 37, and has a length of 21 bases. We instruct primer3 to choose primers that flank the repeat site (because we want to use the PCR product for determining the length of the repeat, which is likely to be polymorphic).

PRIMER_TASK=generic

The PRIMER_TASK tells primer3 which type of primers to pick. You can select typical primers for PCR detection, primers for cloning or for sequencing.

PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_RIGHT_PRIMER=1

We would like to pick a left primer, an internal oligo and a right primer, so we set these flags to 1 (true). In combination with the PRIMER_TASK this tags control which primers are picked.

PRIMER_OPT_SIZE=18

Since our sequence length is rather small (only 108 bases long), we lower the PRIMER_OPT_SIZE from 20 to 18. It is more likely that primer3 will succeed if it aims for smaller primers.

PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21

With the lowering of optimal primer size, it's good to lower the minimum and maximum sizes as well.

PRIMER_MAX_NS_ACCEPTED=1

Since the sequence is short with a non-negligible amount of unknown bases (N's) in it, we make primer3's job easier by allowing it to pick primers that have at most 1 unknown base.

PRIMER_PRODUCT_SIZE_RANGE=75-100

We reduce the product size range from the default of 100-300 because our source sequence is only 108 base pairs long. If we insisted on a product size of 100 base pairs primer3 would have few possibilities to choose from.

P3_FILE_FLAG=1

Because we have a short sequence with many Ns and with a simple sequence repeat that we must amplify, primer3 might fail to pick and primers. Therefore, we want to get the list of primers it considered, so that we could manually pick primers ourselves if primer3 fails to do so. Setting this flag to 1 asks primer3 to output the primers it considered to a forwar primers and reverse primers to output files.

SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21

Normally CA-repeats make poor hybridization probes (because they not specific enough). Therefore, we exclude the CA repeat (which is the TARGET) from consideration for the internal oligo.

PRIMER_EXPLAIN_FLAG=1

We want to see statistics about the oligos and oligo triples (left primer, internal oligo, right primer) that primer3 examined.

=

The '=' character terminates the record.

There were many Boulder-IO input tags that were not specified, which is legal. For the tags with default values, those defaults will be used in the analysis. For the tags with NO default values (for example SEQUENCE_TARGET), the functionality requested by the those tags will be absent. Also, please note that it is not the case that we need to surround a simple sequence repeat every time we want to pick primers!

15. HOW TO MIGRATE TAGS TO IO VERSION 4

With primer3 release 2.0, many Boulder-IO tags were modified and new tags were introduced. The new primer3 tags are designed with the idea in mind that computer scripts and other programs use primer3_core. The modifications make it easier for programs to read and write primer3 input and output.

Furthermore the primer3 default values and the use of PRIMER_WT_TEMPLATE_MISPRIMING and PRIMER_PAIR_WT_TEMPLATE_MISPRIMING have changed in version 2.0.

There are three classes of input: "Sequence" input tags describe a particular input sequence to primer3, and are reset after every Boulder record (now starting with SEQUENCE_). "Global" input tags describe the general parameters that primer3 should use in its searches, and the values of these tags persist between input Boulder records until or unless they are explicitly reset (now starting with PRIMER_). "Program" parameters that deal with the behavior of the primer3 program itself (now starting with P3_). See below for a list of the modified tags.

The handling of PRIMER_TASK changed completely. In the past we used it to tell primer3 what task to perform. Now the task is complemented with PRIMER_PICK_RIGHT_PRIMER, PRIMER_PICK_INTERNAL_OLIGO and PRIMER_PICK_LEFT_PRIMER, which specify which primers are to be picked.

These Tags are modified:

The "per sequence" tags:

NEW VERSION                       - OLD VERSION
----------------------------------------------------------------------------------------                       
SEQUENCE_ID                       - PRIMER_SEQUENCE_ID
SEQUENCE_TEMPLATE                 - SEQUENCE
SEQUENCE_QUALITY                  - PRIMER_SEQUENCE_QUALITY
SEQUENCE_INCLUDED_REGION          - INCLUDED_REGION
SEQUENCE_TARGET                   - TARGET
SEQUENCE_EXCLUDED_REGION          - EXCLUDED_REGION
SEQUENCE_START_CODON_POSITION     - PRIMER_START_CODON_POSITION
SEQUENCE_PRIMER                   - PRIMER_LEFT_INPUT
SEQUENCE_PRIMER_REVCOMP           - PRIMER_RIGHT_INPUT


SEQUENCE_INTERNAL_OLIGO           - PRIMER_INTERNAL_OLIGO_INPUT
SEQUENCE_INTERNAL_EXCLUDED_REGION - PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION

--------------------------------------------------------------------------------
The "global" tags:

NEW VERSION                       - OLD VERSION

PRIMER_TASK                       - PRIMER_TASK (modified use)
PRIMER_PICK_RIGHT_PRIMER          - --- did not exist
PRIMER_PICK_INTERNAL_OLIGO        - PRIMER_PICK_INTERNAL_OLIGO (modified use)
PRIMER_PICK_LEFT_PRIMER           - --- did not exist

PRIMER_PAIR_WT_TEMPLATE_MISPRIMING- PRIMER_PAIR_WT_TEMPLATE_MISPRIMING (modified use)
PRIMER_WT_TEMPLATE_MISPRIMING     - PRIMER_WT_TEMPLATE_MISPRIMING (modified use)
PRIMER_MAX_LIBRARY_MISPRIMING     - PRIMER_MAX_MISPRIMING
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB- PRIMER_INTERNAL_OLIGO_MAX_MISHYB
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING- PRIMER_PAIR_MAX_MISPRIMING
PRIMER_WT_LIBRARY_MISPRIMING      - PRIMER_WT_REP_SIM
PRIMER_INTERNAL_WT_LIBRARY_MISHYB - PRIMER_INTERNAL_WT_REP_SIM
PRIMER_PAIR_WT_LIBRARY_MISPRIMING - PRIMER_PAIR_WT_REP_SIM

PRIMER_MAX_NS_ACCEPTED            - PRIMER_NUM_NS_ACCEPTED
PRIMER_PAIR_MAX_DIFF_TM           - PRIMER_MAX_DIFF_TM
PRIMER_SALT_MONOVALENT            - PRIMER_SALT_CONC
PRIMER_SALT_DIVALENT              - PRIMER_DIVALENT_CONC
PRIMER_TM_FORMULA                 - PRIMER_TM_SANTALUCIA

PRIMER_MAX_SELF_ANY               - PRIMER_SELF_ANY
PRIMER_MAX_SELF_END               - PRIMER_SELF_END

PRIMER_WT_SELF_ANY                - PRIMER_WT_COMPL_ANY
PRIMER_WT_SELF_END                - PRIMER_WT_COMPL_END
PRIMER_PAIR_MAX_COMPL_ANY         - PRIMER_PAIR_ANY
PRIMER_PAIR_MAX_COMPL_END         - PRIMER_PAIR_END

P3_FILE_FLAG                      - PRIMER_FILE_FLAG
P3_COMMENT                        - PRIMER_COMMENT

PRIMER_INTERNAL_SALT_MONOVALENT   - PRIMER_INTERNAL_OLIGO_SALT_CONC
PRIMER_INTERNAL_SALT_DIVALENT     - PRIMER_INTERNAL_OLIGO_DIVALENT_CONC
PRIMER_INTERNAL_WT_SELF_ANY       - PRIMER_IO_WT_COMPL_ANY
PRIMER_INTERNAL_WT_SELF_END       - PRIMER_IO_WT_COMPL_END
PRIMER_INTERNAL_MAX_NS_ACCEPTED   - PRIMER_INTERNAL_OLIGO_NUM_NS
PRIMER_INTERNAL_MAX_SELF_ANY      - PRIMER_INTERNAL_OLIGO_SELF_ANY
PRIMER_INTERNAL_MAX_SELF_END      - PRIMER_INTERNAL_OLIGO_SELF_END

The following tags INTERNAL_OLIGO is replaced by INTERNAL:

PRIMER_INTERNAL_OPT_SIZE          - PRIMER_INTERNAL_OLIGO_OPT_SIZE
PRIMER_INTERNAL_MIN_SIZE          - PRIMER_INTERNAL_OLIGO_MIN_SIZE
PRIMER_INTERNAL_MAX_SIZE          - PRIMER_INTERNAL_OLIGO_MAX_SIZE
PRIMER_INTERNAL_OPT_TM            - PRIMER_INTERNAL_OLIGO_OPT_TM
PRIMER_INTERNAL_MIN_TM            - PRIMER_INTERNAL_OLIGO_MIN_TM
PRIMER_INTERNAL_MAX_TM            - PRIMER_INTERNAL_OLIGO_MAX_TM
PRIMER_INTERNAL_MIN_GC            - PRIMER_INTERNAL_OLIGO_MIN_GC
PRIMER_INTERNAL_OPT_GC_PERCENT    - PRIMER_INTERNAL_OLIGO_OPT_GC_PERCENT
PRIMER_INTERNAL_MAX_GC            - PRIMER_INTERNAL_OLIGO_MAX_GC
PRIMER_INTERNAL_DNTP_CONC         - PRIMER_INTERNAL_OLIGO_DNTP_CONC
PRIMER_INTERNAL_DNA_CONC          - PRIMER_INTERNAL_OLIGO_DNA_CONC
PRIMER_INTERNAL_MAX_POLY_X        - PRIMER_INTERNAL_OLIGO_MAX_POLY_X 
PRIMER_INTERNAL_MISHYB_LIBRARY    - PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY
PRIMER_INTERNAL_MIN_QUALITY       - PRIMER_INTERNAL_OLIGO_MIN_QUALITY

The following tags IO is replaced by INTERNAL:

PRIMER_INTERNAL_WT_TM_GT          - PRIMER_IO_WT_TM_GT
PRIMER_INTERNAL_WT_TM_LT          - PRIMER_IO_WT_TM_LT
PRIMER_INTERNAL_WT_SIZE_LT        - PRIMER_IO_WT_SIZE_LT
PRIMER_INTERNAL_WT_SIZE_GT        - PRIMER_IO_WT_SIZE_GT
PRIMER_INTERNAL_WT_GC_PERCENT_LT  - PRIMER_IO_WT_GC_PERCENT_LT
PRIMER_INTERNAL_WT_GC_PERCENT_GT  - PRIMER_IO_WT_GC_PERCENT_GT
PRIMER_INTERNAL_WT_NUM_NS         - PRIMER_IO_WT_NUM_NS
PRIMER_INTERNAL_WT_SEQ_QUAL       - PRIMER_IO_WT_SEQ_QUAL

--------------------------------------------------------------------------------
OUTPUT TAGS:

There are three big changes on the output:
- INTERNAL_OLIGO is now replaced by INTERNAL.
- The first version is numbered 0.
- The "PRODUCT" tags are renamed
- The errors are modified
- Errors caused by a specific primer are given as PRIMER_LEFT_4_PROBLEMS

Now all primer related output follows the rule: PRIMER_{LEFT,RIGHT,INTERNAL,PAIR}_<j>_<tag_name>. where <j> is an integer from 0 to n, where n is at most the value of PRIMER_NUM_RETURN - 1.

This allows easy scripting by using the underscores _ to split the name. The first part is PRIMER, the second the type of oligo or pair parameters, the third is always a number, starting at 0 and the rest is used by the tags.

That affects also (shown for output number 4):

NEW VERSION                             - OLD VERSION
------------------------------------------------------------------------------------------------------
PRIMER_PAIR_4_PENALTY                   - PRIMER_PAIR_PENALTY_4 (number moved behind PAIR)
PRIMER_PAIR_4_PRODUCT_SIZE              - PRIMER_PRODUCT_SIZE_4 (grouped with PAIR)
PRIMER_PAIR_4_PRODUCT_TM                - PRIMER_PRODUCT_TM_4 (grouped with PAIR)
PRIMER_PAIR_4_PRODUCT_TM_OLIGO_TM_DIFF  - PRIMER_PRODUCT_TM_OLIGO_TM_DIFF_4 (grouped with PAIR)

PRIMER_INTERNAL_EXPLAIN                 - PRIMER_INTERNAL_OLIGO_EXPLAIN

PRIMER_LEFT_4_LIBRARY_MISPRIMING                   - PRIMER_LEFT_4_MISPRIMING_SCORE
PRIMER_INTERNAL_4_LIBRARY_MISHYB                   - PRIMER_INTERNAL_OLIGO_4_MISHYB_SCORE
PRIMER_RIGHT_4_LIBRARY_MISPRIMING                  - PRIMER_RIGHT_4_MISPRIMING_SCORE
PRIMER_PAIR_4_LIBRARY_MISPRIMING                   - PRIMER_PAIR_4_MISPRIMING_SCORE

16. "SEQUENCE" INPUT TAGS

"Sequence" input tags start with SEQUENCE_... and describe a particular input sequence to primer3. They are reset after every Boulder record. Errors in "Sequence" input tags invalidate the current record, but primer3 will continue to process additional records.

SEQUENCE_EXCLUDED_REGION SEQUENCE_INCLUDED_REGION SEQUENCE_PRIMER_REVCOMP
SEQUENCE_FORCE_LEFT_END SEQUENCE_INTERNAL_EXCLUDED_REGION SEQUENCE_QUALITY
SEQUENCE_FORCE_LEFT_START SEQUENCE_INTERNAL_OLIGO SEQUENCE_START_CODON_POSITION
SEQUENCE_FORCE_RIGHT_END SEQUENCE_OVERLAP_JUNCTION_LIST SEQUENCE_TARGET
SEQUENCE_FORCE_RIGHT_START SEQUENCE_PRIMER SEQUENCE_TEMPLATE
SEQUENCE_ID SEQUENCE_PRIMER_PAIR_OK_REGION_LIST  

SEQUENCE_ID (string; default empty)

Short description of the sequence. It is used as an identifier that is reproduced in the output to enable users to identify the source of the chosen primers.

This tag must be present if P3_FILE_FLAG is non-zero.

SEQUENCE_TEMPLATE (nucleotide sequence; default empty)

The sequence from which to choose primers. The sequence must be presented 5' -> 3' (i.e, in the normal way). In general, the bases may be upper or lower case, but lower case letters are treated specially if PRIMER_LOWERCASE_MASKING is set. The entire sequence MUST be all on a single line. (In other words, the sequence cannot span several lines.)

SEQUENCE_INCLUDED_REGION (interval list; default empty)

A sub-region of the given sequence in which to pick primers. For example, often the first dozen or so bases of a sequence are vector, and should be excluded from consideration. The value for this parameter has the form

<start>,<length>

where <start> is the index of the first base to consider, and <length> is the number of subsequent bases in the primer-picking region.

SEQUENCE_TARGET (interval list; default empty)

If one or more targets is specified then a legal primer pair must flank at least one of them. A target might be a simple sequence repeat site (for example a CA repeat) or a single-base-pair polymorphism, or an exon for resequencing. The value should be a space-separated list of

<start>,<length>

pairs where <start> is the index of the first base of a target, and <length> is its length.

See also PRIMER_INSIDE_PENALTY, PRIMER_OUTSIDE_PENALTY. Has a different meaning when PRIMER_TASK=pick_sequencing_primers. See PRIMER_TASK for more information.

SEQUENCE_EXCLUDED_REGION (interval list; default empty)

Primers and oligos may not overlap any region specified in this tag. The associated value must be a space-separated list of

<start>,<length>

pairs where <start> is the index of the first base of the excluded region, and <length> is its length. This tag is useful for tasks such as excluding regions of low sequence quality or for excluding regions containing repetitive elements such as ALUs or LINEs.

SEQUENCE_PRIMER_PAIR_OK_REGION_LIST (semicolon separated list of integer "quadruples"; default empty)

This tag allows detailed specification of possible locations of left and right primers in primer pairs.

The associated value must be a semicolon-separated list of

<left_start>,<left_length>,<right_start>,<right_length>

quadruples. The left primer must be in the region specified by <left_start>,<left_length> and the right primer must be in the region specified by <right_start>,<right_length>. <left_start> and <left_length> specify the location of the left primer in terms of the index of the first base in the region and the length of the region. <right_start> and <right_length> specify the location of the right primer in analogous fashion. As seen in the example below, if no integers are specified for a region then the location of the corresponding primer is not constrained.

Example:

SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=100,50,300,50 ; 900,60,, ; ,,930,100


Specifies that there are three choices:

Left primer in the 50 bp region starting at position 100 AND right primer in the 50 bp region starting at position 300

OR

Left primer in the 60 bp region starting at position 900 (and right primer anywhere)

OR

Right primer in the 100 bp region starting at position 930 (and left primer anywhere)

SEQUENCE_OVERLAP_JUNCTION_LIST (space separated integers; default empty)

If this list is not empty, then the forward OR the reverse primer must overlap one of these junction points by at least PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION nucleotides at the 3' end and at least PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION nucleotides at the 5' end.

In more detail: The junction associated with a given position is the space immediately to the right of that position in the template sequence on the strand given as input.

For example:


SEQUENCE_OVERLAP_JUNCTION_LIST=20
# 1-based indexes
PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION=4

template: atcataggccatgcctgagc^gctacgact

          ok           ...gagc^gcta-3'  (left primer)
          not ok       ...gagc^gct-3'   (left primer)
          ok           3'-ctcg^cgat...  (right pimer)
          not ok        3'-tcg^cgat...  (right primer)

PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=5

         ok           5'-tgagc^gccg...  (left primer)
         not ok        5'-gagc^gccg...  (left primer)
         ok           ...tgagc^gctac-5' (right primer)
         not ok       ...tgagc^gcta-5'  (right primer)

SEQUENCE_INTERNAL_EXCLUDED_REGION (interval list; default empty)

Middle oligos may not overlap any region specified by this tag. The associated value must be a space-separated list of

<start>,<length>

pairs, where <start> is the index of the first base of an excluded region, and <length> is its length. Often one would make Target regions excluded regions for internal oligos.

SEQUENCE_PRIMER (nucleotide sequence; default empty)

The sequence of a left primer to check and around which to design right primers and optional internal oligos. Must be a substring of SEQUENCE_TEMPLATE.

SEQUENCE_INTERNAL_OLIGO (nucleotide sequence; default empty)

The sequence of an internal oligo to check and around which to design left and right primers. Must be a substring of SEQUENCE_TEMPLATE.

SEQUENCE_PRIMER_REVCOMP (nucleotide sequence; default empty)

The sequence of a right primer to check and around which to design left primers and optional internal oligos. Must be a substring of the reverse strand of SEQUENCE_TEMPLATE.

SEQUENCE_START_CODON_POSITION (int; default -2000000)

This parameter should be considered EXPERIMENTAL at this point. Please check the output carefully; some erroneous inputs might cause an error in primer3.

Index of the first base of a start codon. This parameter allows primer3 to select primer pairs to create in-frame amplicons e.g. to create a template for a fusion protein. Primer3 will attempt to select an in-frame left primer, ideally starting at or to the left of the start codon, or to the right if necessary. Negative values of this parameter are legal if the actual start codon is to the left of available sequence. If this parameter is non-negative primer3 signals an error if the codon at the position specified by this parameter is not an ATG. A value less than or equal to -10^6 indicates that primer3 should ignore this parameter.

Primer3 selects the position of the right primer by scanning right from the left primer for a stop codon. Ideally the right primer will end at or after the stop codon.

SEQUENCE_QUALITY (space separated integers; default empty)

A list of space separated integers. There must be exactly one integer for each base in SEQUENCE_TEMPLATE if this argument is non-empty. For example, for the sequence ANNTTCA... SEQUENCE_QUALITY might be 45 10 0 50 30 34 50 67 .... High numbers indicate high confidence in the base called at that position and low numbers indicate low confidence in the base call at that position. This parameter is only relevant if you are using a base calling program that provides quality information (for example phred).

SEQUENCE_FORCE_LEFT_START (int; default -1000000)

Forces the 5' end of the left primer to be at the indicated position. Primers are also picked if they violate certain constraints. The default value indicates that the start of the left primer can be anywhere.

SEQUENCE_FORCE_LEFT_END (int; default -1000000)

Forces the 3' end of the left primer to be at the indicated position. Primers are also picked if they violate certain constraints. The default value indicates that the end of the left primer can be anywhere.

SEQUENCE_FORCE_RIGHT_START (int; default -1000000)

Forces the 5' end of the right primer to be at the indicated position. Primers are also picked if they violate certain constraints. The default value indicates that the start of the right primer can be anywhere.

SEQUENCE_FORCE_RIGHT_END (int; default -1000000)

Forces the 3' end of the right primer to be at the indicated position. Primers are also picked if they violate certain constraints. The default value indicates that the end of the right primer can be anywhere.

17. "GLOBAL" INPUT TAGS

"Global" input tags start with PRIMER_... and describe the general parameters that primer3 should use in its searches. The values of these tags persist between input Boulder records until or unless they are explicitly reset. Errors in "Global" input tags are fatal because they invalidate the basic conditions under which primers are being picked.

Because the laboratory detection step using internal oligos is independent of the PCR amplification procedure, internal oligo tags have defaults that are independent of the parameters that govern the selection of PCR primers. For example, the melting temperature of an oligo used for hybridization might be considerably lower than that used as a PCR primer.

PRIMER_DNA_CONC PRIMER_MAX_END_GC PRIMER_PAIR_WT_PRODUCT_SIZE_LT
PRIMER_DNTP_CONC PRIMER_MAX_END_STABILITY PRIMER_PAIR_WT_PRODUCT_TM_GT
PRIMER_EXPLAIN_FLAG PRIMER_MAX_GC PRIMER_PAIR_WT_PRODUCT_TM_LT
PRIMER_FIRST_BASE_INDEX PRIMER_MAX_HAIRPIN_TH PRIMER_PAIR_WT_PR_PENALTY
PRIMER_GC_CLAMP PRIMER_MAX_LIBRARY_MISPRIMING PRIMER_PAIR_WT_TEMPLATE_MISPRIMING
PRIMER_INSIDE_PENALTY PRIMER_MAX_NS_ACCEPTED PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH
PRIMER_INTERNAL_DNA_CONC PRIMER_MAX_POLY_X PRIMER_PICK_ANYWAY
PRIMER_INTERNAL_DNTP_CONC PRIMER_MAX_SELF_ANY PRIMER_PICK_INTERNAL_OLIGO
PRIMER_INTERNAL_MAX_GC PRIMER_MAX_SELF_ANY_TH PRIMER_PICK_LEFT_PRIMER
PRIMER_INTERNAL_MAX_HAIRPIN_TH PRIMER_MAX_SELF_END PRIMER_PICK_RIGHT_PRIMER
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB PRIMER_MAX_SELF_END_TH PRIMER_PRODUCT_MAX_TM
PRIMER_INTERNAL_MAX_NS_ACCEPTED PRIMER_MAX_SIZE PRIMER_PRODUCT_MIN_TM
PRIMER_INTERNAL_MAX_POLY_X PRIMER_MAX_TEMPLATE_MISPRIMING PRIMER_PRODUCT_OPT_SIZE
PRIMER_INTERNAL_MAX_SELF_ANY PRIMER_MAX_TEMPLATE_MISPRIMING_TH PRIMER_PRODUCT_OPT_TM
PRIMER_INTERNAL_MAX_SELF_ANY_TH PRIMER_MAX_TM PRIMER_PRODUCT_SIZE_RANGE
PRIMER_INTERNAL_MAX_SELF_END PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION PRIMER_QUALITY_RANGE_MAX
PRIMER_INTERNAL_MAX_SELF_END_TH PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION PRIMER_QUALITY_RANGE_MIN
PRIMER_INTERNAL_MAX_SIZE PRIMER_MIN_END_QUALITY PRIMER_SALT_CORRECTIONS
PRIMER_INTERNAL_MAX_TM PRIMER_MIN_GC PRIMER_SALT_DIVALENT
PRIMER_INTERNAL_MIN_GC PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE PRIMER_SALT_MONOVALENT
PRIMER_INTERNAL_MIN_QUALITY PRIMER_MIN_QUALITY PRIMER_SEQUENCING_ACCURACY
PRIMER_INTERNAL_MIN_SIZE PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE PRIMER_SEQUENCING_INTERVAL
PRIMER_INTERNAL_MIN_TM PRIMER_MIN_SIZE PRIMER_SEQUENCING_LEAD
PRIMER_INTERNAL_MISHYB_LIBRARY PRIMER_MIN_THREE_PRIME_DISTANCE PRIMER_SEQUENCING_SPACING
PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME PRIMER_MIN_TM PRIMER_TASK
PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME PRIMER_MISPRIMING_LIBRARY PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT
PRIMER_INTERNAL_OPT_GC_PERCENT PRIMER_MUST_MATCH_FIVE_PRIME PRIMER_THERMODYNAMIC_PARAMETERS_PATH
PRIMER_INTERNAL_OPT_SIZE PRIMER_MUST_MATCH_THREE_PRIME PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT
PRIMER_INTERNAL_OPT_TM PRIMER_NUM_RETURN PRIMER_TM_FORMULA
PRIMER_INTERNAL_SALT_DIVALENT PRIMER_OPT_GC_PERCENT PRIMER_WT_END_QUAL
PRIMER_INTERNAL_SALT_MONOVALENT PRIMER_OPT_SIZE PRIMER_WT_END_STABILITY
PRIMER_INTERNAL_WT_END_QUAL PRIMER_OPT_TM PRIMER_WT_GC_PERCENT_GT
PRIMER_INTERNAL_WT_GC_PERCENT_GT PRIMER_OUTSIDE_PENALTY PRIMER_WT_GC_PERCENT_LT
PRIMER_INTERNAL_WT_GC_PERCENT_LT PRIMER_PAIR_MAX_COMPL_ANY PRIMER_WT_HAIRPIN_TH
PRIMER_INTERNAL_WT_HAIRPIN_TH PRIMER_PAIR_MAX_COMPL_ANY_TH PRIMER_WT_LIBRARY_MISPRIMING
PRIMER_INTERNAL_WT_LIBRARY_MISHYB PRIMER_PAIR_MAX_COMPL_END PRIMER_WT_NUM_NS
PRIMER_INTERNAL_WT_NUM_NS PRIMER_PAIR_MAX_COMPL_END_TH PRIMER_WT_POS_PENALTY
PRIMER_INTERNAL_WT_SELF_ANY PRIMER_PAIR_MAX_DIFF_TM PRIMER_WT_SELF_ANY
PRIMER_INTERNAL_WT_SELF_ANY_TH PRIMER_PAIR_MAX_LIBRARY_MISPRIMING PRIMER_WT_SELF_ANY_TH
PRIMER_INTERNAL_WT_SELF_END PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING PRIMER_WT_SELF_END
PRIMER_INTERNAL_WT_SELF_END_TH PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH PRIMER_WT_SELF_END_TH
PRIMER_INTERNAL_WT_SEQ_QUAL PRIMER_PAIR_WT_COMPL_ANY PRIMER_WT_SEQ_QUAL
PRIMER_INTERNAL_WT_SIZE_GT PRIMER_PAIR_WT_COMPL_ANY_TH PRIMER_WT_SIZE_GT
PRIMER_INTERNAL_WT_SIZE_LT PRIMER_PAIR_WT_COMPL_END PRIMER_WT_SIZE_LT
PRIMER_INTERNAL_WT_TM_GT PRIMER_PAIR_WT_COMPL_END_TH PRIMER_WT_TEMPLATE_MISPRIMING
PRIMER_INTERNAL_WT_TM_LT PRIMER_PAIR_WT_DIFF_TM PRIMER_WT_TEMPLATE_MISPRIMING_TH
PRIMER_LIBERAL_BASE PRIMER_PAIR_WT_IO_PENALTY PRIMER_WT_TM_GT
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS PRIMER_PAIR_WT_LIBRARY_MISPRIMING PRIMER_WT_TM_LT
PRIMER_LOWERCASE_MASKING PRIMER_PAIR_WT_PRODUCT_SIZE_GT  

PRIMER_TASK (string; default generic)

This tag tells primer3 what task to perform. Legal values are:

generic

Design a primer pair (the classic primer3 task) if the PRIMER_PICK_LEFT_PRIMER=1, and PRIMER_PICK_RIGHT_PRIMER=1. In addition, pick an internal hybridization oligo if PRIMER_PICK_INTERNAL_OLIGO=1.

NOTE: If PRIMER_PICK_LEFT_PRIMER=1, PRIMER_PICK_RIGHT_PRIMER=0 and PRIMER_PICK_INTERNAL_OLIGO=1, then behaves similarly to PRIMER_TASK=pick_primer_list.

pick_detection_primers

Deprecated alias for PRIMER_TASK=generic

check_primers

Primer3 only checks the primers provided in SEQUENCE_PRIMER, SEQUENCE_INTERNAL_OLIGO and SEQUENCE_PRIMER_REVCOMP. It is the only task that does not require a sequence. However, if SEQUENCE_TEMPLATE is provided, it is used.

pick_primer_list

Pick all primers in SEQUENCE_TEMPLATE (possibly limited by SEQUENCE_INCLUDED_REGION, SEQUENCE_EXCLUDED_REGION, SEQUENCE_PRIMER_PAIR_OK_REGION_LIST, etc.). Returns the primers sorted by quality starting with the best primers. If PRIMER_PICK_LEFT_PRIMER and PRIMER_PICK_RIGHT_PRIMER is selected primer3 does not to pick primer pairs but generates independent lists of left primers, right primers, and, if requested, internal oligos.

pick_sequencing_primers

Pick primers suited to sequence a region. SEQUENCE_TARGET can be used to indicate several targets. The position of each primer is calculated for optimal sequencing results.

pick_cloning_primers

Pick primers suited to clone a gene were the start nucleotide and the end nucleotide of the PCR fragment must be fixed, for example to clone an ORF. SEQUENCE_INCLUDED_REGION must be used to indicate the first and the last nucleotide. Due to these limitations primer3 can only vary the length of the primers. Set PRIMER_PICK_ANYWAY=1 to obtain primers even if they violate specific constraints.

pick_discriminative_primers

Pick primers suited to select primers which bind with their end at a specific position. This can be used to force the end of a primer to a polymorphic site, with the goal of discriminating between sequence variants. SEQUENCE_INCLUDED_REGION must be used to indicate the last nucleotide of the left (first nucleotide of included region) and the right primer (last nucleotide of included region). Due to these limitations primer3 can only vary the length of the primers. Set PRIMER_PICK_ANYWAY=1 to obtain primers even if they violate specific constraints.

pick_pcr_primers

Deprecated shortcut for the following settings:
PRIMER_TASK=generic
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_RIGHT_PRIMER=1

WARNING: this task changes the values of PRIMER_PICK_LEFT_PRIMER, PRIMER_PICK_INTERNAL_OLIGO, and PRIMER_PICK_RIGHT_PRIMER in a way that is not obvious by looking at the input.

pick_pcr_primers_and_hyb_probe

Deprecated shortcut for the following settings:
PRIMER_TASK=generic
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_RIGHT_PRIMER=1

WARNING: this task changes the values of PRIMER_PICK_LEFT_PRIMER, PRIMER_PICK_INTERNAL_OLIGO, and PRIMER_PICK_RIGHT_PRIMER in a way that is not obvious by looking at the input.

pick_left_only

Deprecated shortcut for the following settings:
PRIMER_TASK=generic
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_RIGHT_PRIMER=0

WARNING: this task changes the values of PRIMER_PICK_LEFT_PRIMER, PRIMER_PICK_INTERNAL_OLIGO, and PRIMER_PICK_RIGHT_PRIMER in a way that is not obvious by looking at the input.

pick_right_only

Deprecated shortcut for the following settings:
PRIMER_TASK=generic
PRIMER_PICK_LEFT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_RIGHT_PRIMER=1

WARNING: this task changes the values of PRIMER_PICK_LEFT_PRIMER, PRIMER_PICK_INTERNAL_OLIGO, and PRIMER_PICK_RIGHT_PRIMER in a way that is not obvious by looking at the input.

pick_hyb_probe_only

Deprecated shortcut for the following settings:
PRIMER_TASK=generic
PRIMER_PICK_LEFT_PRIMER=0
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_RIGHT_PRIMER=0

WARNING: this task changes the values of PRIMER_PICK_LEFT_PRIMER, PRIMER_PICK_INTERNAL_OLIGO, and PRIMER_PICK_RIGHT_PRIMER in a way that is not obvious by looking at the input.

PRIMER_PICK_LEFT_PRIMER (boolean; default 1)

If the associated value = 1 (non-0), then primer3 will attempt to pick left primers.

PRIMER_PICK_INTERNAL_OLIGO (boolean; default 0)

If the associated value = 1 (non-0), then primer3 will attempt to pick an internal oligo (hybridization probe to detect the PCR product).

PRIMER_PICK_RIGHT_PRIMER (boolean; default 1)

If the associated value = 1 (non-0), then primer3 will attempt to pick a right primer.

PRIMER_NUM_RETURN (int; default 5)

The maximum number of primer (pairs) to return. Primer pairs returned are sorted by their "quality", in other words by the value of the objective function (where a lower number indicates a better primer pair). Caution: setting this parameter to a large value will increase running time.

PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION (int; default 4)

The 3' end of the left OR the right primer must overlap one of the junctions in SEQUENCE_OVERLAP_JUNCTION_LIST by this amount. See details in SEQUENCE_OVERLAP_JUNCTION_LIST.

PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION (int; default 7)

The 5' end of the left OR the right primer must overlap one of the junctions in SEQUENCE_OVERLAP_JUNCTION_LIST by this amount. See details in SEQUENCE_OVERLAP_JUNCTION_LIST.

PRIMER_MUST_MATCH_FIVE_PRIME (ambiguous nucleotide sequence; default empty)

Discards all primers which do not match this match sequence at the 5' end. (New in v. 2.3.6, added by A. Untergasser.)

The match sequence must be 5 nucletides long and can contain the following letters:

    N   Any nucleotide

    A   Adenine
    G   Guanine
    C   Cytosine
    T   Thymine

    R   Purine (A or G)
    Y   Pyrimidine (C or T)
    W   Weak (A or T)
    S   Strong (G or C)
    M   Amino (A or C)
    K   Keto (G or T)
    B   Not A (G or C or T)
    H   Not G (A or C or T)
    D   Not C (A or G or T)
    V   Not T (A or G or C)
Any primer which will not match the entire match sequence at th 5' end will be discarded and not evaluated. Setting strict requirements here will result in low quality primers due to the high numbers of primers discarded at this step.

Example 1:
PRIMER_MUST_MATCH_FIVE_PRIME=tgnnn

Could result in the following matching:
    tgcatgattggatacgtttga
    |||||
    tgnnn

 -> This primer would be used.


    attcgattctccccggtatc
      |||
    tgnnn

 -> This primer would be discarded.


Example 2:
PRIMER_MUST_MATCH_FIVE_PRIME=hnnnn

Could result in the following matching:
    tgcatgattggatacgtttga
    |||||
    hnnnn

 -> This primer would be used.


    ggctgatgaaggaaagcaag
     ||||
    hnnnn

 -> This primer would be discarded.


This parameter would force all primers selected by primer3 to not have guanosine at the 5' end of any primer which could be useful to avoid quenching of flourochromes.

PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME (ambiguous nucleotide sequence; default empty)

Equivalent parameter of PRIMER_MUST_MATCH_FIVE_PRIME for the internal oligo.

PRIMER_MUST_MATCH_THREE_PRIME (ambiguous nucleotide sequence; default empty)

Discards all primers which do not match this match sequence at the 3' end. Similar parameter to PRIMER_MUST_MATCH_FIVE_PRIME, but limits the 3' end. (New in v. 2.3.6, added by A. Untergasser.)

The match sequence must be 5 nucletides long and can contain the following letters:

    N   Any nucleotide

    A   Adenine
    G   Guanine
    C   Cytosine
    T   Thymine

    R   Purine (A or G)
    Y   Pyrimidine (C or T)
    W   Weak (A or T)
    S   Strong (G or C)
    M   Amino (A or C)
    K   Keto (G or T)
    B   Not A (G or C or T)
    H   Not G (A or C or T)
    D   Not C (A or G or T)
    V   Not T (A or G or C)
Any primer which will not match the entire match sequence at th 3' end will be discarded and not evaluated. Setting strict requirements here will result in low quality primers due to the high numbers of primers discarded at this step.

Example 1:
PRIMER_MUST_MATCH_FIVE_PRIME=nnnga

Could result in the following matching:
    tgcatgattggatacgtttga
                    |||||
                    nnnga

 -> This primer would be used.


    attcgattctccccggtatc
                   |||
                   nnnga

 -> This primer would be discarded.

PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME (ambiguous nucleotide sequence; default empty)

Equivalent parameter of PRIMER_MUST_MATCH_THREE_PRIME for the internal oligo.

PRIMER_PRODUCT_SIZE_RANGE (size range list; default 100-300)

The associated values specify the lengths of the product that the user wants the primers to create, and is a space separated list of elements of the form

<x>-<y>

where an <x>-<y> pair is a legal range of lengths for the product. For example, if one wants PCR products to be between 100 to 150 bases (inclusive) then one would set this parameter to 100-150. If one desires PCR products in either the range from 100 to 150 bases or in the range from 200 to 250 bases then one would set this parameter to 100-150 200-250.

Primer3 favors product-size ranges to the left side of the parameter string. Primer3 will return legal primers pairs in the first range regardless the value of the objective function for pairs in subsequent ranges. Only if there are an insufficient number of primers in the first range will primer3 return primers in a subsequent range.

For those with primarily a computational background, the PCR product size is the size (in base pairs) of the DNA fragment that would be produced by the PCR reaction on the given sequence template. This would, of course, include the primers themselves.

PRIMER_PRODUCT_OPT_SIZE (int; default 0)

The optimum size for the PCR product. 0 indicates that there is no optimum product size. This parameter influences primer pair selection only if PRIMER_PAIR_WT_PRODUCT_SIZE_GT or PRIMER_PAIR_WT_PRODUCT_SIZE_LT is non-0.
A non-0 value for this parameter will likely increase calculation time, so set this only if a product size near a specific value is truly important.

PRIMER_PAIR_WT_PRODUCT_SIZE_LT (float; default 0.0)

Penalty weight for products shorter than PRIMER_PRODUCT_OPT_SIZE.

PRIMER_PAIR_WT_PRODUCT_SIZE_GT (float; default 0.0)

Penalty weight for products longer than PRIMER_PRODUCT_OPT_SIZE.

PRIMER_MIN_SIZE (int; default 18)

Minimum acceptable length of a primer. Must be greater than 0 and less than or equal to PRIMER_MAX_SIZE.

PRIMER_INTERNAL_MIN_SIZE (int; default 18)

Equivalent parameter of PRIMER_MIN_SIZE for the internal oligo.

PRIMER_OPT_SIZE (int; default 20)

Optimum length (in bases) of a primer. Primer3 will attempt to pick primers close to this length.

PRIMER_INTERNAL_OPT_SIZE (int; default 20)

Equivalent parameter of PRIMER_OPT_SIZE for the internal oligo.

PRIMER_MAX_SIZE (int; default 27)

Maximum acceptable length (in bases) of a primer. Currently this parameter cannot be larger than 35. This limit is governed by maximum oligo size for which primer3's melting-temperature is valid.

PRIMER_INTERNAL_MAX_SIZE (int; default 27)

Equivalent parameter of PRIMER_MAX_SIZE for the internal oligo.

PRIMER_WT_SIZE_LT (float; default 1.0)

Penalty weight for primers shorter than PRIMER_OPT_SIZE.

PRIMER_INTERNAL_WT_SIZE_LT (float; default 1.0)

Equivalent parameter of PRIMER_WT_SIZE_LT for the internal oligo.

PRIMER_WT_SIZE_GT (float; default 1.0)

Penalty weight for primers longer than PRIMER_OPT_SIZE.

PRIMER_INTERNAL_WT_SIZE_GT (float; default 1.0)

Equivalent parameter of PRIMER_INTERNAL_WT_SIZE_GT for the internal oligo.

PRIMER_MIN_GC (float; default 20.0)

Minimum allowable percentage of Gs and Cs in any primer.

PRIMER_INTERNAL_MIN_GC (float; default 20.0)

Equivalent parameter of PRIMER_MIN_GC for the internal oligo.

PRIMER_OPT_GC_PERCENT (float; default 50.0)

Optimum GC percent. This parameter influences primer selection only if PRIMER_WT_GC_PERCENT_GT or PRIMER_WT_GC_PERCENT_LT are non-0.

PRIMER_INTERNAL_OPT_GC_PERCENT (float; default 50.0)

Equivalent parameter of PRIMER_OPT_GC_PERCENT for the internal oligo.

PRIMER_MAX_GC (float; default 80.0)

Maximum allowable percentage of Gs and Cs in any primer generated by Primer.

PRIMER_INTERNAL_MAX_GC (float; default 80.0)

Equivalent parameter of PRIMER_MAX_GC for the internal oligo.

PRIMER_WT_GC_PERCENT_LT (float; default 0.0)

Penalty weight for primers with GC percent lower than PRIMER_OPT_GC_PERCENT.

PRIMER_INTERNAL_WT_GC_PERCENT_LT (float; default 0.0)

Equivalent parameter of PRIMER_WT_GC_PERCENT_LT for the internal oligo.

PRIMER_WT_GC_PERCENT_GT (float; default 0.0)

Penalty weight for primers with GC percent higher than PRIMER_OPT_GC_PERCENT.

PRIMER_INTERNAL_WT_GC_PERCENT_GT (float; default 0.0)

Equivalent parameter of PRIMER_WT_GC_PERCENT_GT for the internal oligo.

PRIMER_GC_CLAMP (int; default 0)

Require the specified number of consecutive Gs and Cs at the 3' end of both the left and right primer. (This parameter has no effect on the internal oligo if one is requested.)

PRIMER_MAX_END_GC (int; default 5)

The maximum number of Gs or Cs allowed in the last five 3' bases of a left or right primer.

PRIMER_MIN_TM (float; default 57.0)

Minimum acceptable melting temperature (Celsius) for a primer oligo.

PRIMER_INTERNAL_MIN_TM (float; default 57.0)

Equivalent parameter of PRIMER_MIN_TM for the internal oligo.

PRIMER_OPT_TM (float; default 60.0)

Optimum melting temperature (Celsius) for a primer. Primer3 will try to pick primers with melting temperatures are close to this temperature. The oligo melting temperature formula used can be specified by user. Please see PRIMER_TM_FORMULA for more information.

PRIMER_INTERNAL_OPT_TM (float; default 60.0)

Equivalent parameter of PRIMER_OPT_TM for the internal oligo.

PRIMER_MAX_TM (float; default 63.0)

Maximum acceptable melting temperature (Celsius) for a primer oligo.

PRIMER_INTERNAL_MAX_TM (float; default 63.0)

Equivalent parameter of PRIMER_MAX_TM for the internal oligo.

PRIMER_PAIR_MAX_DIFF_TM (float; default 100.0)

Maximum acceptable (unsigned) difference between the melting temperatures of the left and right primers.

PRIMER_WT_TM_LT (float; default 1.0)

Penalty weight for primers with Tm lower than PRIMER_OPT_TM.

PRIMER_INTERNAL_WT_TM_LT (float; default 1.0)

Equivalent parameter of PRIMER_WT_TM_LT for the internal oligo.

PRIMER_WT_TM_GT (float; default 1.0)

Penalty weight for primers with Tm over PRIMER_OPT_TM.

PRIMER_INTERNAL_WT_TM_GT (float; default 1.0)

Equivalent parameter of PRIMER_WT_TM_GT for the internal oligo.

PRIMER_PAIR_WT_DIFF_TM (float; default 0.0)

Penalty weight for the TM difference between the left primer and the right primer.

PRIMER_PRODUCT_MIN_TM (float; default -1000000.0)

The minimum allowed melting temperature of the amplicon. Please see the documentation on PRIMER_PRODUCT_MAX_TM for details.

PRIMER_PRODUCT_OPT_TM (float; default 0.0)

The optimum melting temperature for the PCR product. 0 indicates that there is no optimum temperature.

PRIMER_PRODUCT_MAX_TM (float; default 1000000.0)

The maximum allowed melting temperature of the amplicon. Primer3 calculates product Tm calculated using the formula from Bolton and McCarthy, PNAS 84:1390 (1962) as presented in Sambrook, Fritsch and Maniatis, Molecular Cloning, p 11.46 (1989, CSHL Press).

   Tm = 81.5 + 16.6(log10([Na+])) + .41*(%GC) - 600/length

Where [Na+] is the molar sodium concentration, (%GC) is the percent of Gs and Cs in the sequence, and length is the length of the sequence.

A similar formula is used by the prime primer selection program in GCG (http://www.gcg.com), which instead uses 675.0 / length in the last term (after F. Baldino, Jr, M.-F. Chesselet, and M.E. Lewis, Methods in Enzymology 168:766 (1989) eqn (1) on page 766 without the mismatch and formamide terms). The formulas here and in Baldino et al. assume Na+ rather than K+. According to J.G. Wetmur, Critical Reviews in BioChem. and Mol. Bio. 26:227 (1991) 50 mM K+ should be equivalent in these formulae to .2 M Na+. Primer3 uses the same salt concentration value for calculating both the primer melting temperature and the oligo melting temperature. If you are planning to use the PCR product for hybridization later this behavior will not give you the Tm under hybridization conditions.

PRIMER_PAIR_WT_PRODUCT_TM_LT (float; default 0.0)

Penalty weight for products with a Tm lower than PRIMER_PRODUCT_OPT_TM.

PRIMER_PAIR_WT_PRODUCT_TM_GT (float; default 0.0)

Penalty weight for products with a Tm higher than PRIMER_PRODUCT_OPT_TM.

PRIMER_TM_FORMULA (int; default 1)

Specifies details of melting temperature calculation. (New in v. 1.1.0, added by Maido Remm and Triinu Koressaar.)

A value of 0 directs primer3 to a backward compatible calculation (in other words, the only calculation available in previous version of primer3).

This backward compatible calculation uses the table of thermodynamic parameters in the paper [Breslauer KJ, Frank R, Blöcker H and Marky LA (1986) "Predicting DNA duplex stability from the base sequence" Proc Natl Acad Sci 83:4746-50 http://dx.doi.org/10.1073/pnas.83.11.3746], and the method in the paper [Rychlik W, Spencer WJ and Rhoads RE (1990) "Optimization of the annealing temperature for DNA amplification in vitro", Nucleic Acids Res 18:6409-12 http://dx.doi.org/10.1093/nar/18.21.6409].

A value of 1 (*RECOMMENDED*) directs primer3 to use the table of thermodynamic values and the method for melting temperature calculation suggested in the paper [SantaLucia JR (1998) "A unified view of polymer, dumbbell and oligonucleotide DNA nearest-neighbor thermodynamics", Proc Natl Acad Sci 95:1460-65 http://dx.doi.org/10.1073/pnas.95.4.1460].

Use tag PRIMER_SALT_CORRECTIONS, to specify the salt correction method for melting temperature calculation.

Example of calculating the melting temperature of an oligo if PRIMER_TM_FORMULA=1 and PRIMER_SALT_CORRECTIONS=1 recommended values):

primer=CGTGACGTGACGGACT

Using default salt and DNA concentrations we have

Tm = deltaH/(deltaS + R*ln(C/4))

where R is the gas constant (1.987 cal/K mol) and C is the DNA concentration.

deltaH(predicted) =
  = dH(CG) + dH(GT) + dH(TG) + .. + dH(CT) +
     + dH(init.w.term.GC) + dH(init.w.term.AT) =

  = -10.6 + (-8.4) + (-8.5) + .. + (-7.8) + 0.1 + 2.3  =

  = -128.8 kcal/mol

where 'init.w.term GC' and 'init.w.term AT' are two initiation parameters for duplex formation: 'initiation with terminal GC' and 'initiation with terminal AT'

deltaS(predicted) =
  = dS(CG) + dS(GT) + dS(TG) + .. + dS(CT) +
    + dS(init.w.term.GC) + dS(init.w.term.AT) =

  = -27.2 + (-22.4) + (-22.7) + .. + (-21.0) + (-2.8) + 4.1 =
 
  = -345.2 cal/k*mol

deltaS(salt corrected) = 
  = deltaS(predicted) + 0.368*15(NN pairs)*ln(0.05M monovalent cations) =
  = -361.736

Tm = -128.800/(-361.736+1.987*ln((5*10^(-8))/4)) =
   = 323.704 K

Tm(C) = 323.704 - 273.15 = 50.554 C

PRIMER_SALT_MONOVALENT (float; default 50.0)

The millimolar (mM) concentration of monovalent salt cations (usually KCl) in the PCR. Primer3 uses this argument to calculate oligo and primer melting temperatures. Use tag PRIMER_SALT_DIVALENT and PRIMER_INTERNAL_SALT_DIVALENT to specify the concentrations of divalent cations (in which case you also should also set tag PRIMER_DNTP_CONC to a reasonable value).

PRIMER_INTERNAL_SALT_MONOVALENT (float; default 50.0)

Equivalent parameter of PRIMER_SALT_MONOVALENT for the internal oligo.

PRIMER_SALT_DIVALENT (float; default 1.5)

The millimolar concentration of divalent salt cations (usually MgCl^(2+)) in the PCR. (New in v. 1.1.0, added by Maido Remm and Triinu Koressaar)

Primer3 converts concentration of divalent cations to concentration of monovalent cations using formula suggested in the paper [Ahsen von N, Wittwer CT, Schutz E (2001) "Oligonucleotide Melting Temperatures under PCR Conditions: Nearest-Neighbor Corrections for Mg^(2+), Deoxynucleotide Triphosphate, and Dimethyl Sulfoxide Concentrations with Comparison to Alternative Empirical Formulas", Clinical Chemistry 47:1956-61 http://www.clinchem.org/cgi/content/full/47/11/1956].

[Monovalent cations] = [Monovalent cations] + 120*(([divalent cations] - [dNTP])^0.5)

In addition, if the specified concentration of dNTPs (PRIMER_DNTP_CONC) is larger than the concentration of divalent cations (PRIMER_SALT_DIVALENT) then the effect of the divalent cations is not considered. The concentration of dNTPs is considered in the formula above because of some magnesium is bound by the dNTP. The adjusted concentration of monovalent cations is used in the calculation of oligo/primer melting temperature, PCR product melting temperature, the stability of oligo dimers and secondary structures (when PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT is 1), and the stability of ectopic annealing of oligos to template (when PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT is 1). If PRIMER_SALT_DIVALENT > 0.0, be sure to set tag PRIMER_DNTP_CONC to specify the concentration of dNTPs.

PRIMER_INTERNAL_SALT_DIVALENT (float; default 0.0)

Equivalent parameter of PRIMER_SALT_DIVALENT for the internal oligo.

PRIMER_DNTP_CONC (float; default 0.6)

The millimolar concentration of the sum of all deoxyribonucleotide triphosphates. A reaction mix containing 0.2 mM ATP, 0.2 mM CTP, 0.2 mM GTP and 0.2 mM TTP would have a PRIMER_DNTP_CONC=0.8. This argument is considered for oligo and primer melting temperatures, for PCR product melting temperature, or for secondary structure calculations only if PRIMER_SALT_DIVALENT is > 0.0. See PRIMER_SALT_DIVALENT.

PRIMER_INTERNAL_DNTP_CONC (float; default 0.0)

Parameter for internal oligos analogous to PRIMER_DNTP_CONC.

PRIMER_SALT_CORRECTIONS (int; default 1)

Specifies the salt correction formula for the melting temperature calculation. (New in v. 1.1.0, added by Maido Remm and Triinu Koressaar)

A value of 0 directs primer3 to use the the salt correction formula in the paper [Schildkraut, C, and Lifson, S (1965) "Dependence of the melting temperature of DNA on salt concentration", Biopolymers 3:195-208 (not available on-line)]. This was the formula used in older versions of primer3.

A value of 1 (*RECOMMENDED*) directs primer3 to use the salt correction formula in the paper [SantaLucia JR (1998) "A unified view of polymer, dumbbell and oligonucleotide DNA nearest-neighbor thermodynamics", Proc Natl Acad Sci 95:1460-65 http://dx.doi.org/10.1073/pnas.95.4.1460]

A value of 2 directs primer3 to use the salt correction formula in the paper [Owczarzy, R., Moreira, B.G., You, Y., Behlke, M.A., and Walder, J.A. (2008). Predicting stability of DNA duplexes in solutions containing magnesium and monovalent cations. Biochemistry 47, 5336-5353 http://dx.doi.org/10.1021/bi702363u] following recommendations in the paper [Ahsen, v.N., Wittwer, C.T., and Schütz, E. (2010). Monovalent and divalent salt correction algorithms for Tm prediction-recommendations for Primer3 usage. Brief Bioinform 12, 514 http://dx.doi.org/10.1093/bib/bbq081].

For all values of PRIMER_SALT_CORRECTIONS, Primer3 also considers the values of the tags PRIMER_SALT_DIVALENT, PRIMER_INTERNAL_SALT_DIVALENT, PRIMER_DNTP_CONC, and PRIMER_INTERNAL_DNTP_CONC.

PRIMER_DNA_CONC (float; default 50.0)

A value to use as nanomolar (nM) concentration of each annealing oligo over the course the PCR. Primer3 uses this argument to esimate oligo melting temperatures. This parameter corresponds to 'c' in equation (ii) of the paper [SantaLucia (1998) A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Proc Natl Acad Sci 95:1460-1465 http://www.pnas.org/content/95/4/1460.full.pdf+html], where a suitable value (for a lower initial concentration of template) is "empirically determined".

The default (50nM) works well with the standard protocol used at the Whitehead/MIT Center for Genome Research--0.5 microliters of 20 micromolar concentration for each primer in a 20 microliter reaction with 10 nanograms template, 0.025 units/microliter Taq polymerase in 0.1 mM each dNTP, 1.5mM MgCl2, 50mM KCl, 10mM Tris-HCL (pH 9.3) using 35 cycles with an annealing temperature of 56 degrees Celsius.

The value of this parameter is less than the actual concentration of oligos in the initial reaction mix because it is the concentration of annealing oligos, which in turn depends on the amount of template (including PCR product) in a given cycle. This concentration increases a great deal during a PCR; fortunately PCR seems quite robust for a variety of oligo melting temperatures.
See ADVICE FOR PICKING PRIMERS.

PRIMER_INTERNAL_DNA_CONC (float; default 50.0)

Equivalent parameter of PRIMER_DNA_CONC for the internal oligo.

PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT (boolean; default 1)

If the associated value = 1, then primer3 will use thermodynamic models to calculate the the propensity of oligos to form hairpins and dimers.

PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT (boolean; default 0)

If the associated value = 1, then primer3 will use thermodynamic models to calculate the the propensity of oligos to anneal to undesired sites in the template sequence.

PRIMER_THERMODYNAMIC_PARAMETERS_PATH (string; default ./primer3_config)

This tag specifies the path to the directory that contains all the parameter files used by the thermodynamic approach. In Linux, there are two default locations that are tested if this tag is not defined: ./primer3_config/ and /opt/primer3_config/. For Windows, there is only one default location: .\primer3_config\.

PRIMER_MAX_SELF_ANY (decimal, 9999.99; default 8.00)

PRIMER_MAX_SELF_ANY describes the tendency of a primer to bind to itself (interfering with target sequence binding). It will score ANY binding occurring within the entire primer sequence.
It is the maximum allowable local alignment score when testing a single primer for (local) self-complementarity and the maximum allowable local alignment score when testing for complementarity between left and right primers. Local self-complementarity is taken to predict the tendency of primers to anneal to each other without necessarily causing self-priming in the PCR. The scoring system gives 1.00 for complementary bases, -0.25 for a match of any base (or N) with an N, -1.00 for a mismatch, and -2.00 for a gap. Only single-base-pair gaps are allowed. For example, the alignment

   5' ATCGNA 3'
      || | |
   3' TA-CGT 5'
is allowed (and yields a score of 1.75), but the alignment 
   5' ATCCGNA 3'
      ||  | |
   3' TA--CGT 5'

is not considered. Scores are non-negative, and a score of 0.00 indicates that there is no reasonable local alignment between two oligos.

PRIMER_MAX_SELF_ANY_TH (decimal, 9999,99; default 47.00)

The same as PRIMER_MAX_SELF_ANY but all calculations are based on thermodynamical approach. The melting temperature of the most stable structure is calculated. To calculate secondary structures nearest-neighbor parameters for perfect matches, single internal mismatches, terminal mismatches, dangling ends have been used. Also parameters for increments for length dependence of bulge and internal loops have been used. This parameter is calculated only if PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1. The default value is 10 degrees lower than the default value of PRIMER_MIN_TM. For example, the alignment width length 15nt

  5' ATTAGATAGAGCATC 3'
  3' TAATCTATCTCGTAG 5'
is allowed (and yields a melting temperature of 32.1493 width by default primer3 parameters), but the alignment 
     T        C
  5'  GCGGCCGC GCGC 3'
  3'  CGCCGGCG CGCG 5'
     A        A

is not considered (Tm=57.0997 and the length of oligo is 14nt). Thermodynamical parameters and methods for finding the most stable structure are described in following papers:


Predicting secondary structures can improve primer design by eliminating sequences with high possibility to form alternative secondary structures.

PRIMER_INTERNAL_MAX_SELF_ANY (decimal, 9999.99; default 12.00)

Equivalent parameter of PRIMER_MAX_SELF_ANY for the internal oligo.

PRIMER_INTERNAL_MAX_SELF_ANY_TH (decimal, 9999.99; default 47.00)

Equivalent parameter of PRIMER_MAX_SELF_ANY_TH for the internal oligo.

PRIMER_PAIR_MAX_COMPL_ANY (decimal, 9999.99; default 8.00)

PRIMER_PAIR_MAX_COMPL_ANY describes the tendency of the left primer to bind to the right primer. It is similar to PRIMER_MAX_SELF_ANY.

PRIMER_PAIR_MAX_COMPL_ANY_TH (decimal, 9999.99; default 47.00)

PRIMER_PAIR_MAX_COMPL_ANY_TH describes the tendency of the left primer to bind to the right primer. It is similar to PRIMER_MAX_SELF_ANY_TH.

PRIMER_WT_SELF_ANY (float; default 0.0)

Penalty weight for the individual primer self binding value as in PRIMER_MAX_SELF_ANY.

PRIMER_WT_SELF_ANY_TH (float; default 0.0)

Penalty weight for the individual primer self binding value as in PRIMER_MAX_SELF_ANY_TH.

PRIMER_INTERNAL_WT_SELF_ANY (float; default 0.0)

Equivalent parameter of PRIMER_WT_SELF_ANY for the internal oligo.

PRIMER_INTERNAL_WT_SELF_ANY_TH (float; default 0.0)

Equivalent parameter of PRIMER_WT_SELF_ANY_TH for the internal oligo.

PRIMER_PAIR_WT_COMPL_ANY (float; default 0.0)

Penalty weight for the binding value of the primer pair as in PRIMER_MAX_SELF_ANY.

PRIMER_PAIR_WT_COMPL_ANY_TH (float; default 0.0)

Penalty weight for the binding value of the primer pair as in PRIMER_MAX_SELF_ANY_TH.

PRIMER_MAX_SELF_END (decimal, 9999.99; default 3.00)

PRIMER_MAX_SELF_END tries to bind the 3'-END to a identical primer and scores the best binding it can find. This is critical for primer quality because it allows primers use itself as a target and amplify a short piece (forming a primer-dimer). These primers are then unable to bind and amplify the target sequence.
PRIMER_MAX_SELF_END is the maximum allowable 3'-anchored global alignment score when testing a single primer for self-complementarity, and the maximum allowable 3'-anchored global alignment score when testing for complementarity between left and right primers. The 3'-anchored global alignment score is taken to predict the likelihood of PCR-priming primer-dimers, for example

   5' ATGCCCTAGCTTCCGGATG 3'
                ||| |||||
             3' AAGTCCTACATTTAGCCTAGT 5'
or 
   5` AGGCTATGGGCCTCGCGA 3'
                  ||||||
               3' AGCGCTCCGGGTATCGGA 5'

The scoring system is as for the Maximum Complementarity argument. In the examples above the scores are 7.00 and 6.00 respectively. Scores are non-negative, and a score of 0.00 indicates that there is no reasonable 3'-anchored global alignment between two oligos. In order to estimate 3'-anchored global alignments for candidate primers and primer pairs, Primer assumes that the sequence from which to choose primers is presented 5'->3'. It is nonsensical to provide a larger value for this parameter than for the Maximum (local) Complementarity parameter (PRIMER_MAX_SELF_ANY) because the score of a local alignment will always be at least as great as the score of a global alignment.

PRIMER_MAX_SELF_END_TH (decimal 9999.99; default 47.00)

Same as PRIMER_MAX_SELF_END but is based on thermodynamical approach - the stability of structure is analyzed. The value of tag is expressed as melting temperature. See PRIMER_MAX_SELF_ANY_TH for details.

PRIMER_INTERNAL_MAX_SELF_END (decimal 9999.99; default 12.00)

PRIMER_INTERNAL_MAX_SELF_END is meaningless when applied to internal oligos used for hybridization-based detection, since primer-dimer will not occur. We recommend that PRIMER_INTERNAL_MAX_SELF_END be set at least as high as PRIMER_INTERNAL_MAX_SELF_ANY.

PRIMER_INTERNAL_MAX_SELF_END_TH (decimal 9999.99; default 47.00)

Same as PRIMER_INTERNAL_MAX_SELF_END but for calculating the score (melting temperature of structure) thermodynamical approach is used.

PRIMER_PAIR_MAX_COMPL_END (decimal, 9999.99; default 3.00)

PRIMER_PAIR_MAX_COMPL_END tries to bind the 3'-END of the left primer to the right primer and scores the best binding it can find. It is similar to PRIMER_MAX_SELF_END.

PRIMER_PAIR_MAX_COMPL_END_TH (decimal, 9999.99; default 47.00)

Same as PRIMER_PAIR_MAX_COMPL_END but for calculating the score (melting temperature of structure) thermodynamical approach is used.

PRIMER_WT_SELF_END (float; default 0.0)

Penalty weight for the individual primer self binding value as in PRIMER_MAX_SELF_END.

PRIMER_WT_SELF_END_TH (float; default 0.0)

Penalty weight for the individual primer self binding value as in PRIMER_MAX_SELF_END_TH

PRIMER_INTERNAL_WT_SELF_END (float; default 0.0)

Equivalent parameter of PRIMER_WT_SELF_END for the internal oligo.

PRIMER_INTERNAL_WT_SELF_END_TH (float; default 0.0)

Equivalent parameter of PRIMER_WT_SELF_END_TH for the internal oligo.

PRIMER_PAIR_WT_COMPL_END (float; default 0.0)

Penalty weight for the binding value of the primer pair as in PRIMER_MAX_SELF_END.

PRIMER_PAIR_WT_COMPL_END_TH (float; default 0.0)

Penalty weight for the binding value of the primer pair as in PRIMER_MAX_SELF_END_TH.

PRIMER_MAX_HAIRPIN_TH (float; default 47.0)

This is the most stable monomer structure of internal oligo calculated by thermodynamic approach. The hairpin loops, bulge loops, internal loops, internal single mismatches, dangling ends, terminal mismatches have been considered. This parameter is calculated only if PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1. The default value is 10 degrees lower than the default value of PRIMER_MIN_TM. For example the structure:

      -///------\\\-
   5' ACGCTGTGCTGCGA 3'
with melting temperature 53.7263 (calculated according to by default values of primer3) and 
      //////----\\\\\\
   5' CCGCAGTAAGCTGCGG 3'

with melting temperature 71.0918 (calculated according to by default values of primer3) For details about papers used for calculating hairpins see PRIMER_MAX_SELF_ANY_TH

PRIMER_INTERNAL_MAX_HAIRPIN_TH (float; default 47.0)

The most stable monomer structure of internal oligo calculated by thermodynamic approach. See PRIMER_MAX_HAIRPIN_TH for details.

PRIMER_WT_HAIRPIN_TH (float; default 0.0)

Penalty weight for the individual primer hairpin structure value as in PRIMER_MAX_HAIRPIN_TH.

PRIMER_INTERNAL_WT_HAIRPIN_TH (float; default 0.0)

Penalty weight for the most stable primer hairpin structure value as in PRIMER_INTERNAL_MAX_HAIRPIN_TH.

PRIMER_MAX_END_STABILITY (float, 999.9999; default 100.0)

The maximum stability for the last five 3' bases of a left or right primer. Bigger numbers mean more stable 3' ends. The value is the maximum delta G (kcal/mol) for duplex disruption for the five 3' bases as calculated using the nearest-neighbor parameter values specified by the option of PRIMER_TM_FORMULA

For example if the table of thermodynamic parameters suggested by SantaLucia 1998, DOI:10.1073/pnas.95.4.1460 is used the deltaG values for the most stable and for the most labile 5mer duplex are 6.86 kcal/mol (GCGCG) and 0.86 kcal/mol (TATAT) respectively.

If the table of thermodynamic parameters suggested by Breslauer et al. 1986, 10.1073/pnas.83.11.3746 is used the deltaG values for the most stable and for the most labile 5mer are 13.4 kcal/mol (GCGCG) and 4.6 kcal/mol (TATAC) respectively.

PRIMER_WT_END_STABILITY (float; default 0.0)

Penalty factor for the calculated maximum stability for the last five 3' bases of a left or right primer.

PRIMER_MAX_NS_ACCEPTED (int; default 0)

Maximum number of unknown bases (N) allowable in any primer.

PRIMER_INTERNAL_MAX_NS_ACCEPTED (int; default 0)

Equivalent parameter of PRIMER_MAX_NS_ACCEPTED for the internal oligo.

PRIMER_WT_NUM_NS (float; default 0.0)

Penalty weight for the number of Ns in the primer.

PRIMER_INTERNAL_WT_NUM_NS (float; default 0.0)

Equivalent parameter of PRIMER_WT_NUM_NS for the internal oligo.

PRIMER_MAX_POLY_X (int; default 5)

The maximum allowable length of a mononucleotide repeat, for example AAAAAA.

PRIMER_INTERNAL_MAX_POLY_X (int; default 5)

Equivalent parameter of PRIMER_MAX_POLY_X for the internal oligo.

PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE (int; default -1)

When returning multiple primer pairs, the minimum number of base pairs between the 3' ends of any two left primers.

Primers with 3' ends at positions e.g. 30 and 31 in the template sequence have a three-prime distance of 1.

In addition to positive values, the values -1 and 0 are acceptable and have special interpretations:

-1 indicates that a given left primer can appear in multiple primer pairs returned by primer3. This is the default behavior.

0 indicates that a left primer is acceptable if it was not already used. In other words, two left primers are allowed to have the same 3' position provided their 5' positions differ.

For n > 0: A left primer is acceptable if:

NOT(3' end of left primer closer than n to the 3' end of a previously used left primer)

PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE (int; default -1)

Analogous to PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE, but for right primers.

PRIMER_MIN_THREE_PRIME_DISTANCE (int; default -1)

A "convenience" tag that simultaneously sets PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE and PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE

For example

PRIMER_MIN_THREE_PRIME_DISTANCE=3
is equivalent to 
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=3
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=3

It is an error to specify both PRIMER_MIN_THREE_PRIME_DISTANCE and either PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE or PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE in the same input record.

PRIMER_PICK_ANYWAY (boolean; default 0)

If true use primer provided in SEQUENCE_PRIMER, SEQUENCE_PRIMER_REVCOMP, or SEQUENCE_INTERNAL_OLIGO even if it violates specific constraints.

PRIMER_LOWERCASE_MASKING (int; default 0)

This option allows for intelligent design of primers in sequence in which masked regions (for example repeat-masked regions) are lower-cased. (New in v. 1.1.0, added by Maido Remm and Triinu Koressaar)

A value of 1 directs primer3 to reject primers overlapping lowercase a base exactly at the 3' end.

This property relies on the assumption that masked features (e.g. repeats) can partly overlap primer, but they cannot overlap the 3'-end of the primer. In other words, lowercase bases at other positions in the primer are accepted, assuming that the masked features do not influence the primer performance if they do not overlap the 3'-end of primer.

PRIMER_EXPLAIN_FLAG (boolean; default 0)

If this flag is 1 (non-0), produce PRIMER_LEFT_EXPLAIN, PRIMER_RIGHT_EXPLAIN, PRIMER_INTERNAL_EXPLAIN and/or PRIMER_PAIR_EXPLAIN output tags as appropriate. These output tags are intended to provide information on the number of oligos and primer pairs that primer3 examined and counts of the number discarded for various reasons. If -format_output is set similar information is produced in the user-oriented output.

PRIMER_LIBERAL_BASE (boolean; default 0)

This parameter provides a quick-and-dirty way to get primer3 to accept IUB / IUPAC codes for ambiguous bases (i.e. by changing all unrecognized bases to N). If you wish to include an ambiguous base in an oligo, you must set PRIMER_MAX_NS_ACCEPTED to a 1 (non-0) value.

Perhaps '-' and '* ' should be squeezed out rather than changed to 'N', but currently they simply get converted to N's. The authors invite user comments.

PRIMER_FIRST_BASE_INDEX (int; default 0)

This parameter is the index of the first base in the input sequence. For input and output using 1-based indexing (such as that used in GenBank and to which many users are accustomed) set this parameter to 1. For input and output using 0-based indexing set this parameter to 0. (This parameter also affects the indexes in the contents of the files produced when the primer file flag is set.)

PRIMER_MAX_TEMPLATE_MISPRIMING (decimal, 9999.99; default -1.00)

The maximum allowed similarity to ectopic sites in the template. A negative value means do not check. The scoring system is the same as used for PRIMER_MAX_LIBRARY_MISPRIMING, except that an ambiguity code in the template is never treated as a consensus (see PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS).

PRIMER_MAX_TEMPLATE_MISPRIMING_TH (decimal, 9999.99; default -1.00)

Similar to PRIMER_MAX_TEMPLATE_MISPRIMING but assesses alternative binding sites in the template using thermodynamic models (when PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1). This parameter specifies the maximum allowed melting temperature of an oligo (primer) at an "ectopic" site within the template sequence; 47.0 would be a reasonable choice if PRIMER_MIN_TM is 57.0.

PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING (decimal, 9999.99; default -1.00)

The maximum allowed summed similarity of both primers to ectopic sites in the template. A negative value means do not check. The scoring system is the same as used for PRIMER_PAIR_MAX_LIBRARY_MISPRIMING, except that an ambiguity code in the template is never treated as a consensus (see PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS). Primer3 does not check the similarity of hybridization oligos (internal oligos) to locations outside of the amplicon.

PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH (decimal, 9999.99; default -1.00)

The maximum allowed summed melting temperatures of both primers at ectopic sites within the template (with the two primers in an orientation that would allow PCR amplification.) The melting temperatures are calculated as for PRIMER_MAX_TEMPLATE_MISPRIMING_TH.

PRIMER_WT_TEMPLATE_MISPRIMING (float; default 0.0)

Penalty for a single primer binding to the template sequence.

The use of this Tag is modified from primer3 version 2.0 on: The values used with the older versions have to be multiplied by the factor 100 to have the same effect.

PRIMER_WT_TEMPLATE_MISPRIMING_TH (float; default 0.0)

Penalty for a single primer binding to the template sequence (thermodynamic approach, when PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1).

PRIMER_PAIR_WT_TEMPLATE_MISPRIMING (float; default 0.0)

Penalty for a primer pair binding to the template sequence.

The use of this Tag is modified from primer3 version 2.0 on: The values used with the older versions have to be multiplied by the factor 100 to have the same effect.

PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH (float; default 0.0)

Penalty for a primer pair binding to the template sequence (thermodynamic approach, when PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1).

PRIMER_MISPRIMING_LIBRARY (string; default empty)

The name of a file containing a nucleotide sequence library of sequences to avoid amplifying (for example repetitive sequences, or possibly the sequences of genes in a gene family that should not be amplified.) The file must be in (a slightly restricted) FASTA format (W. B. Pearson and D.J. Lipman, PNAS 85:8 pp 2444-2448 [1988]); we briefly discuss the organization of this file below. If this parameter is specified then primer3 locally aligns each candidate primer against each library sequence and rejects those primers for which the local alignment score times a specified weight (see below) exceeds PRIMER_MAX_LIBRARY_MISPRIMING. (The maximum value of the weight is arbitrarily set to 100.0.)

Each sequence entry in the FASTA-format file must begin with an "id line" that starts with '>'. The contents of the id line is "slightly restricted" in that primer3 parses everything after any optional asterisk ('*') as a floating point number to use as the weight mentioned above. If the id line contains no asterisk then the weight defaults to 1.0. The alignment scoring system used is the same as for calculating complementarity among oligos (e.g. PRIMER_MAX_SELF_ANY), except for the handling of IUB/IUPAC ambiguity codes (discussed below).

The remainder of an entry contains the sequence as lines following the id line up until a line starting with '>' or the end of the file. Whitespace and newlines are ignored. Characters 'A', 'T', 'G', 'C', 'a', 't', 'g', 'c' and IUB/IUPAC 'ambiguity' codes ('R, 'Y', 'K', 'M', 'S', 'W', 'N', including lower case) are retained. For technical reasons the length of the sequence must be >= 3. Of course, sequences of length < 10 or so are probably useless, but will be accepted without complaint.

WARNING: always set PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 if any sequence in the library contains strings of 'N's: NNNNNNNNNNNNNNNNNNNN.
NOWWW
There are no restrictions on line length.

An empty value for this parameter indicates that no repeat library should be used and "turns off" the use of a previously specified library.

Repbase (J. Jurka, A.F.A. Smit, C. Pethiyagoda, and others, 1995-1996, ftp://ftp.ncbi.nih.gov/repository/repbase/) is an excellent source of repeat sequences and pointers to the literature. (The Repbase files need to be converted to Fasta format before they can be used by primer3.)

PRIMER_INTERNAL_MISHYB_LIBRARY (string; default empty)

Similar to PRIMER_MISPRIMING_LIBRARY, except that the event we seek to avoid is hybridization of the internal oligo to sequences in this library rather than priming from them.

PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS (boolean; default 0)

If set to 1, treat ambiguity codes as if they were consensus codes when matching oligos to mispriming or mishyb libraries. For example, if this flag is set, then a C in an oligo will be scored as a perfect match to an S in a library sequence, as will a G in the oligo. More importantly, though, any base in an oligo will be scored as a perfect match to an N in the library. This is very bad if the library contains strings of Ns, as no oligo will be legal (and it will take a long time to find this out). So unless you know for sure that your library does not have runs of Ns (or Xs), then set this flag to 0.

PRIMER_MAX_LIBRARY_MISPRIMING (decimal, 9999.99; default 12.00)

The maximum allowed weighted similarity with any sequence in PRIMER_MISPRIMING_LIBRARY.

PRIMER_INTERNAL_MAX_LIBRARY_MISHYB (decimal,9999.99; default 12.00)

Similar to PRIMER_MAX_LIBRARY_MISPRIMING except that this parameter applies to the similarity of candidate internal oligos to the library specified in PRIMER_INTERNAL_MISHYB_LIBRARY.

PRIMER_PAIR_MAX_LIBRARY_MISPRIMING (decimal, 9999.99; default 24.00)

The maximum allowed sum of similarities of a primer pair (one similarity for each primer) with any single sequence in PRIMER_MISPRIMING_LIBRARY. Library sequence weights are not used in computing the sum of similarities.

PRIMER_WT_LIBRARY_MISPRIMING (float; default 0.0)

Penalty for a single primer binding to any single sequence in PRIMER_MISPRIMING_LIBRARY.

PRIMER_INTERNAL_WT_LIBRARY_MISHYB (float; default 0.0)

Equivalent parameter of PRIMER_WT_LIBRARY_MISPRIMING for the internal oligo.

PRIMER_PAIR_WT_LIBRARY_MISPRIMING (float; default 0.0)

Penalty for a primer pair binding to any single sequence in PRIMER_MISPRIMING_LIBRARY.

PRIMER_MIN_QUALITY (int; default 0)

The minimum sequence quality (as specified by SEQUENCE_QUALITY) allowed within a primer.

PRIMER_INTERNAL_MIN_QUALITY (int; default 0)

Equivalent parameter of PRIMER_MIN_QUALITY for the internal oligo.

PRIMER_MIN_END_QUALITY (int; default 0)

The minimum sequence quality (as specified by SEQUENCE_QUALITY) allowed within the 5' pentamer of a primer. Note that there is no PRIMER_INTERNAL_MIN_END_QUALITY.

PRIMER_QUALITY_RANGE_MIN (int; default 0)

The minimum legal sequence quality (used for error checking of PRIMER_MIN_QUALITY and PRIMER_MIN_END_QUALITY).

PRIMER_QUALITY_RANGE_MAX (int; default 100)

The maximum legal sequence quality (used for error checking of PRIMER_MIN_QUALITY and PRIMER_MIN_END_QUALITY).

PRIMER_WT_SEQ_QUAL (float; default 0.0)

Penalty weight for the sequence quality of the primer.

PRIMER_INTERNAL_WT_SEQ_QUAL (float; default 0.0)

Equivalent parameter of PRIMER_WT_SEQ_QUAL for the internal oligo.

PRIMER_PAIR_WT_PR_PENALTY (float; default 1.0)

Penalty factor for the sum of the left and the right primer added to the pair penalty. Setting this value below 1.0 will increase running time.

As PRIMER_PAIR_WT_PR_PENALTY or the per-primer penalties it multiplies become lower with respect to various pair penalties (for example PRIMER_PAIR_WT_PRODUCT_SIZE_LT PRIMER_PAIR_WT_PRODUCT_SIZE_GT PRIMER_PAIR_WT_DIFF_TM, etc.) the running time of the search for primer pairs is likely to grow substantially. The reason is that the search algorithm must calculate the penalty for more primer pairs (as opposed to excluding them based on the penalties of the individual oligos).

PRIMER_PAIR_WT_IO_PENALTY (float; default 0.0)

Penalty factor for the internal oligo added to the pair penalty.

PRIMER_INSIDE_PENALTY (float; default -1.0)

Non-default values are valid only for sequences with 0 or 1 target regions. If the primer is part of a pair that spans a target and overlaps the target, then multiply this value times the number of nucleotide positions by which the primer overlaps the (unique) target to get the 'position penalty'. The effect of this parameter is to allow primer3 to include overlap with the target as a term in the objective function.

PRIMER_OUTSIDE_PENALTY (float; default 0.0)

Non-default values are valid only for sequences with 0 or 1 target regions. If the primer is part of a pair that spans a target and does not overlap the target, then multiply this value times the number of nucleotide positions from the 3' end to the (unique) target to get the 'position penalty'. The effect of this parameter is to allow primer3 to include nearness to the target as a term in the objective function.

PRIMER_WT_POS_PENALTY (float; default 1.0)

Penalty for the primer which do not overlap the target.

PRIMER_SEQUENCING_LEAD (int; default 50)

Defines the space from the 3'end of the primer to the point were the trace signals are readable. Value only used if PRIMER_TASK=pick_sequencing_primers.

PRIMER_SEQUENCING_SPACING (int; default 500)

Defines the space from the 3'end of the primer to the 3'end of the next primer on the same strand. Value only used if PRIMER_TASK=pick_sequencing_primers.

PRIMER_SEQUENCING_INTERVAL (int; default 250)

Defines the space from the 3'end of the primer to the 3'end of the next primer on the reverse strand. Value only used if PRIMER_TASK=pick_sequencing_primers.

PRIMER_SEQUENCING_ACCURACY (int; default 20)

Defines the space from the calculated position of the 3'end to both sides in which primer3plus picks the best primer. Value only used if PRIMER_TASK=pick_sequencing_primers.

PRIMER_WT_END_QUAL (float; default 0.0)

PRIMER_INTERNAL_WT_END_QUAL (float; default 0.0)

18. "PROGRAM" INPUT TAGS

"Program" input tags start with P3_... describe the parameters that deal with the behavior of the primer3 program itself.)

P3_FILE_ID (string; default empty)

This tag is only valid in primer3 setting files. It should be used to identify the purpose of the settings files it appears in. It is always printed out on the output of primer3. See also also the command line flag -echo_settings_file, which causes the entire settings file to be echoed in primer3_core's output.

P3_FILE_FLAG (boolean; default 0)

If the associated value = 1 (non-0), then primer3 creates one or more output files for each input SEQUENCE_TEMPLATE. File <sequence_id>.for lists all acceptable left primers for <sequence_id>, and <sequence_id>.rev lists all acceptable right primers for <sequence_id>, where <sequence_id> is the value of the SEQUENCE_ID tag (which must be supplied). If internal oligos are requested, primer3 produces a file <sequence_id>.int, which lists all acceptable internal oligos. Obviously, <sequence_id> needs to be a string that will work as a file name.

See also the pick_primer_list argument to PRIMER_TASK, which offers similar functionality on stdout.

P3_COMMENT (string; default empty)

The value of this tag is ignored. It can be used to annotate input.

19. HOW PRIMER3 CALCULATES THE PENALTY VALUE

In essense, the penalty values define what is the best primer pair. The calculation of penalty values takes into consideration penalty weights, which allow one to fine-tune the selection of primers to specific needs.

This section will explain the selection process of primers by primer3. In general the selection is a multi step process:

In the first step, primer3 evaluates every primer that can be picked in the region of interest, possibly subject to constraints due to target regions, product size ranges, and so forth, that might preclude the use of primers in the eventually selected primer pairs. In this pass the hard limits are tested like PRIMER_MAX_GC or PRIMER_MIN_TM. Primers with a GC lower than PRIMER_MAX_GC or a Tm higher than PRIMER_MIN_TM are memorized, the primers which fail in one of these tests are excluded. Primer3 can be forced to use primers failing to pass this test by setting PRIMER_PICK_ANYWAY to one (only available for primers provided by the user).

In the second step, primer3 calculates a penalty for each primer. This penalty is the only score by which primer3 evaluates the primers It is also provided as output PRIMER_LEFT_4_PENALTY, PRIMER_INTERNAL_4_PENALTY and PRIMER_RIGHT_4_PENALTY (shown for the primer set 4). For each primer, it is calculated like that:

Left Primers:

PRIMER_LEFT_4_PENALTY =

   If PRIMER_LEFT_4_TM > PRIMER_OPT_TM then this is added (+):

       + PRIMER_WT_TM_GT * ( PRIMER_LEFT_4_TM - PRIMER_OPT_TM )

   If PRIMER_LEFT_4_TM < PRIMER_OPT_TM then this is added (+):

       + PRIMER_WT_TM_LT * ( PRIMER_OPT_TM - PRIMER_LEFT_4_TM )


   If PRIMER_LEFT_4_GC_PERCENT > PRIMER_OPT_GC_PERCENT then 
       this is added (+):

       + PRIMER_WT_GC_PERCENT_GT *
            ( PRIMER_LEFT_4_GC_PERCENT - PRIMER_OPT_GC_PERCENT )

   If PRIMER_LEFT_4_GC_PERCENT < PRIMER_OPT_GC_PERCENT then
       this is added (+):

       + PRIMER_WT_GC_PERCENT_LT *
            ( PRIMER_OPT_GC_PERCENT - PRIMER_LEFT_4_GC_PERCENT )


   The following section uses <primer length> as part of 
   the term which is given as output in 
   PRIMER_LEFT_4=position,<primer length> 

   If <primer length> > PRIMER_OPT_SIZE then
       this is added (+):

       + PRIMER_WT_SIZE_GT *
            ( <primer length> - PRIMER_OPT_SIZE )

   If <primer length> < PRIMER_OPT_SIZE then
       this is added (+):

       + PRIMER_WT_SIZE_LT *
            ( PRIMER_OPT_SIZE - <primer length> )


   If the primer does not overlap a target then
       this is added (+):
	
       + PRIMER_WT_POS_PENALTY * PRIMER_LEFT_4_POSITION_PENALTY


   These are allways added (+) to the penalty
   (if the thermodynamic approach is used then the part in italic
      is substituted with text below this calculation):

    + PRIMER_WT_SELF_ANY * PRIMER_LEFT_4_SELF_ANY

       + PRIMER_WT_SELF_END * PRIMER_LEFT_4_SELF_END

       + PRIMER_WT_TEMPLATE_MISPRIMING *
            PRIMER_LEFT_4_TEMPLATE_MISPRIMING

       + PRIMER_WT_END_STABILITY * PRIMER_LEFT_4_END_STABILITY

       + PRIMER_WT_NUM_NS * <numbers of N in the selected primer>

       + PRIMER_WT_LIBRARY_MISPRIMING * PRIMER_LEFT_4_LIBRARY_MISPRIMING

       + PRIMER_WT_SEQ_QUAL *
            ( PRIMER_QUALITY_RANGE_MAX - 
              PRIMER_LEFT_4_MIN_SEQ_QUALITY )
      
      If the thermodynamic approach is used then the part of italic in
          the above calculation is replaced by this:

 
     If ((PRIMER_LEFT_4_TM - 5) ≤ PRIMER_LEFT_4_SELF_ANY_TH) then is added (+):
      + PRIMER_WT_SELF_ANY_TH *
                  (PRIMER_LEFT_4_SELF_ANY_TH - (PRIMER_LEFT_4_TM - 5 - 1))
    else if ((PRIMER_LEFT_4_TM - 5) > PRIMER_LEFT_4_SELF_ANY_TH) then is added (+):
      + PRIMER_WT_SELF_ANY_TH *
            (1/(PRIMER_LEFT_4_TM - 5 + 1 - PRIMER_LEFT_4_SELF_ANY_TH));

    If ((PRIMER_LEFT_4_TM - 5) ≤ PRIMER_LEFT_4_SELF_END_TH) then is added (+):
     + PRIMER_WT_SELF_END_TH *
               (PRIMER_LEFT_4_SELF_END_TH - (PRIMER_LEFT_4_TM - 5 - 1))
    else if ((PRIMER_LEFT_4_TM - 5) > PRIMER_LEFT_4_SELF_END_TH) then is added (+):
     + PRIMER_WT_SELF_END_TH *
       (1/(PRIMER_LEFT_4_TM - 5 + 1 - PRIMER_LEFT_4_SELF_ANY_TH));

    If ((PRIMER_LEFT_4_TM - 5) ≤ PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH) then is added (+):
     + PRIMER_WT_TEMPLATE_MISPRIMING_TH *
       (PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH - (PRIMER_LEFT_4_TM - 5 - 1))
    else if ((PRIMER_LEFT_4_TM - 5) > PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH) then is added (+):
     + PRIMER_WT_TEMPLATE_MISPRIMING_TH *
      (1/(PRIMER_LEFT_4_TM - 5 + 1 - PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH));

    If ((PRIMER_LEFT_4_TM - 5) ≤ PRIMER_LEFT_4_HAIRPIN_TH) then is added (+):
      + PRIMER_WT_HAIRPIN_TH *
             (PRIMER_LEFT_4_HAIRPIN_TH - (PRIMER_LEFT_4_TM - 5 - 1))
    else if ((PRIMER_LEFT_4_TM - 5) > PRIMER_LEFT_4_HAIRPIN_TH) then is added (+):
      + PRIMER_WT_HAIRPIN_TH *
            (1/(PRIMER_LEFT_4_TM - 5 + 1 - PRIMER_LEFT_4_HAIRPIN_TH));

Right Primers (identical to Left Primers):

PRIMER_RIGHT_4_PENALTY =

   If PRIMER_RIGHT_4_TM > PRIMER_OPT_TM then 
       this is added (+):

       + PRIMER_WT_TM_GT * ( PRIMER_RIGHT_4_TM - PRIMER_OPT_TM )

   If PRIMER_RIGHT_4_TM < PRIMER_OPT_TM then 
       this is added (+):

       + PRIMER_WT_TM_LT * ( PRIMER_OPT_TM - PRIMER_RIGHT_4_TM )


   If PRIMER_RIGHT_4_GC_PERCENT > PRIMER_OPT_GC_PERCENT then 
       this is added (+):

       + PRIMER_WT_GC_PERCENT_GT *
            ( PRIMER_RIGHT_4_GC_PERCENT - PRIMER_OPT_GC_PERCENT )

   If PRIMER_RIGHT_4_GC_PERCENT < PRIMER_OPT_GC_PERCENT then 
       this is added (+):

       + PRIMER_WT_GC_PERCENT_LT *
            ( PRIMER_OPT_GC_PERCENT - PRIMER_RIGHT_4_GC_PERCENT )


   The following section uses <primer length> as part of 
   the term which is given as output in 
   PRIMER_RIGHT_4=position,<primer length> 

   If <primer length> > PRIMER_OPT_SIZE then 
       this is added (+):

       + PRIMER_WT_SIZE_GT *
            ( <primer length> - PRIMER_OPT_SIZE )

   If <primer length> < PRIMER_OPT_SIZE then 
       this is added (+):

       + PRIMER_WT_SIZE_LT *
            ( PRIMER_OPT_SIZE - <primer length> )


   If the primer does not overlap a target then 
       this is added (+):

       + PRIMER_WT_POS_PENALTY * PRIMER_RIGHT_4_POSITION_PENALTY


   These are allways added (+) to the penalty
   (if the thermodynamic approach is used then the part in italic
      is substituted with text below this calculation):

    + PRIMER_WT_SELF_ANY * PRIMER_RIGHT_4_SELF_ANY

       + PRIMER_WT_SELF_END * PRIMER_RIGHT_4_SELF_END

       + PRIMER_WT_TEMPLATE_MISPRIMING *
            PRIMER_RIGHT_4_TEMPLATE_MISPRIMING

       + PRIMER_WT_END_STABILITY * PRIMER_RIGHT_4_END_STABILITY

       + PRIMER_WT_NUM_NS * <numbers of N in the selected primer>

       + PRIMER_WT_LIBRARY_MISPRIMING * PRIMER_RIGHT_4_LIBRARY_MISPRIMING

       + PRIMER_WT_SEQ_QUAL *
            ( PRIMER_QUALITY_RANGE_MAX - 
              PRIMER_RIGHT_4_MIN_SEQ_QUALITY )
     
     If the thermodynamic approach is used then the part of italic in
         the above calculation is replaced by this:


    If ((PRIMER_RIGHT_4_TM - 5) ≤ PRIMER_RIGHT_4_SELF_ANY_TH) then is added (+):
      + PRIMER_WT_SELF_ANY_TH *
                  (PRIMER_RIGHT_4_SELF_ANY_TH - (PRIMER_RIGHT_4_TM - 5 - 1))
    else if ((PRIMER_RIGHT_4_TM - 5) > PRIMER_RIGHT_4_SELF_ANY_TH) then is added (+):
      + PRIMER_WT_SELF_ANY_TH *
            (1/(PRIMER_RIGHT_4_TM - 5 + 1 - PRIMER_RIGHT_4_SELF_ANY_TH));

    If ((PRIMER_RIGHT_4_TM - 5) ≤ PRIMER_RIGHT_4_SELF_END_TH) then is added (+):
     + PRIMER_WT_SELF_END_TH *
               (PRIMER_RIGHT_4_SELF_END_TH - (PRIMER_RIGHT_4_TM - 5 - 1))
    else if ((PRIMER_RIGHT_4_TM - 5) > PRIMER_RIGHT_4_SELF_END_TH) then is added (+):
     + PRIMER_WT_SELF_END_TH *
               (1/(PRIMER_RIGHT_4_TM - 5 + 1 - PRIMER_RIGHT_4_SELF_ANY_TH));

    If ((PRIMER_RIGHT_4_TM - 5) ≤ PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH) then is added (+):
      + PRIMER_WT_TEMPLATE_MISPRIMING_TH *
       (PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH - (PRIMER_RIGHT_4_TM - 5 - 1))
    else if ((PRIMER_RIGHT_4_TM - 5) > PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH) then is added (+):
      + PRIMER_WT_TEMPLATE_MISPRIMING_TH *
      (1/(PRIMER_RIGHT_4_TM - 5 + 1 - PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH));

    If ((PRIMER_RIGHT_4_TM - 5) ≤ PRIMER_RIGHT_4_HAIRPIN_TH) then is added (+):
      + PRIMER_WT_HAIRPIN_TH *
             (PRIMER_RIGHT_4_HAIRPIN_TH - (PRIMER_RIGHT_4_TM - 5 - 1))
    else if ((PRIMER_RIGHT_4_TM - 5) > PRIMER_RIGHT_4_HAIRPIN_TH) then is added (+):
      + PRIMER_WT_HAIRPIN_TH *
            (1/(PRIMER_RIGHT_4_TM - 5 + 1 - PRIMER_RIGHT_4_HAIRPIN_TH));

Internal Oligos:

PRIMER_INTERNAL_4_PENALTY =

   If PRIMER_INTERNAL_4_TM > PRIMER_INTERNAL_OPT_TM then
       this is added (+):

       + PRIMER_INTERNAL_WT_TM_GT *
            ( PRIMER_INTERNAL_4_TM - PRIMER_INTERNAL_OPT_TM )

   If PRIMER_INTERNAL_4_TM < PRIMER_INTERNAL_OPT_TM then
       this is added (+):

       + PRIMER_INTERNAL_WT_TM_LT *
            ( PRIMER_INTERNAL_OPT_TM - PRIMER_INTERNAL_4_TM )


   If PRIMER_INTERNAL_4_GC_PERCENT > PRIMER_INTERNAL_OPT_GC_PERCENT
       then this is added (+):

       + PRIMER_INTERNAL_WT_GC_PERCENT_GT *
            ( PRIMER_INTERNAL_4_GC_PERCENT - 
              PRIMER_INTERNAL_OPT_GC_PERCENT )

   If PRIMER_INTERNAL_4_GC_PERCENT < PRIMER_INTERNAL_OPT_GC_PERCENT
       then this is added (+):

       + PRIMER_INTERNAL_WT_GC_PERCENT_LT *
            ( PRIMER_INTERNAL_OPT_GC_PERCENT -
              PRIMER_INTERNAL_4_GC_PERCENT )


   The following section uses <primer length> as part of 
   the term which is given as output in 
   PRIMER_INTERNAL_4=position,<primer length> 

   If <primer length> > PRIMER_INTERNAL_OPT_SIZE then 
       this is added (+):

       + PRIMER_INTERNAL_WT_SIZE_GT *
            ( <primer length> - PRIMER_INTERNAL_OPT_SIZE )

   If <primer length> < PRIMER_INTERNAL_OPT_SIZE then
       this is added (+):

       + PRIMER_INTERNAL_WT_SIZE_LT *
            ( PRIMER_INTERNAL_OPT_SIZE - <primer length> )


   These are always added (+) to the penalty:
   (if the thermodynamic approach is used then the part in italic
      is substituted with text below this calculation):
      
  + PRIMER_INTERNAL_WT_SELF_ANY * PRIMER_INTERNAL_4_SELF_ANY

     + PRIMER_INTERNAL_WT_SELF_END * PRIMER_INTERNAL_4_SELF_END

     + PRIMER_INTERNAL_WT_NUM_NS *
          <numbers of N in the selected primer>

     + PRIMER_INTERNAL_WT_LIBRARY_MISHYB *
          PRIMER_INTERNAL_4_LIBRARY_MISHYB

     + PRIMER_INTERNAL_WT_SEQ_QUAL * 
          ( PRIMER_QUALITY_RANGE_MAX - 
            PRIMER_INTERNAL_4_MIN_SEQ_QUALITY )

   If the thermodynamic approach is used then the part of italic in
   the above calculation is replaced by this:

   If ((PRIMER_INTERNAL_4_TM - 5) ≤ PRIMER_INTERNAL_4_SELF_ANY_TH) then is added (+):
    + PRIMER_INTERNAL_WT_SELF_ANY_TH *
        (PRIMER_INTERNAL_4_SELF_ANY_TH - (PRIMER_INTERNAL_4_TM - 5 - 1))
    else if ((PRIMER_INTERNAL_4_TM - 5) > PRIMER_INTERNAL_4_SELF_ANY_TH) then is added (+):
    + PRIMER_INTERNAL_WT_SELF_ANY_TH *
        (1/(PRIMER_INTERNAL_4_TM - 5 + 1 - PRIMER_INTERNAL_4_SELF_ANY_TH));

    If ((PRIMER_INTERNAL_4_TM - 5) ≤ PRIMER_INTERNAL_4_SELF_END_TH) then is added (+):
     + PRIMER_INTERNAL_WT_SELF_END_TH *
         (PRIMER_INTERNAL_4_SELF_END_TH - (PRIMER_INTERNAL_4_TM - 5 - 1))
    else if ((PRIMER_INTERNAL_4_TM - 5) > PRIMER_INTERNAL_4_SELF_END_TH) then is added (+):
      + PRIMER_INTERNAL_WT_SELF_END_TH *
          (1/(PRIMER_INTERNAL_4_TM - 5 + 1 - PRIMER_INTERNAL_4_SELF_ANY_TH));

    If ((PRIMER_INTERNAL_4_TM - 5) ≤ PRIMER_INTERNAL_4_HAIRPIN_TH) then is added (+):
      + PRIMER_INTERNAL_WT_HAIRPIN_TH *
        (PRIMER_INTERNAL_4_HAIRPIN_TH - (PRIMER_INTERNAL_4_TM - 5 - 1))
    else if ((PRIMER_INTERNAL_4_TM - 5) > PRIMER_INTERNAL_4_HAIRPIN_TH) then is added (+):
      + PRIMER_INTERNAL_WT_HAIRPIN_TH *
        (1/(PRIMER_INTERNAL_4_TM - 5 + 1 - PRIMER_INTERNAL_4_HAIRPIN_TH));

The primers are then sorted by penalty and primer3 tries to pick the primers with the lowest penalty. For the PRIMER_TASK pick_primer_list or pick_sequencing_primers the selection ends at this point. If primer pairs have to be selected, a PRIMER_PAIR_4_PENALTY is calculated: 

PRIMER_PAIR_4_PENALTY =

   To the pair penalty are at first the single primer penalties 
   added (+):

       + PRIMER_PAIR_WT_PR_PENALTY *
            ( PRIMER_LEFT_4_PENALTY + PRIMER_RIGHT_4_PENALTY )


   If internal oligo is picked then this is added (+):

       + PRIMER_PAIR_WT_IO_PENALTY * PRIMER_INTERNAL_4_PENALTY
	

   If PRIMER_PAIR_4_PRODUCT_TM > PRIMER_PRODUCT_OPT_TM then
       this is added (+):

       + PRIMER_PAIR_WT_PRODUCT_TM_GT *
            ( PRIMER_PAIR_4_PRODUCT_TM - PRIMER_PRODUCT_OPT_TM )

   If PRIMER_PAIR_4_PRODUCT_TM < PRIMER_PRODUCT_OPT_TM then
       this is added (+):

       + PRIMER_PAIR_WT_PRODUCT_TM_LT *
            ( PRIMER_PRODUCT_OPT_TM - PRIMER_PAIR_4_PRODUCT_TM )


   If PRIMER_PAIR_4_PRODUCT_SIZE > PRIMER_PRODUCT_OPT_SIZE then
       this is added (+):

       + PRIMER_PAIR_WT_PRODUCT_SIZE_GT *
            ( PRIMER_PAIR_4_PRODUCT_SIZE - PRIMER_PRODUCT_OPT_SIZE )

   If PRIMER_PAIR_4_PRODUCT_SIZE < PRIMER_PRODUCT_OPT_SIZE then
       this is added (+):

       + PRIMER_PAIR_WT_PRODUCT_SIZE_LT *
            ( PRIMER_PRODUCT_OPT_SIZE - PRIMER_PAIR_4_PRODUCT_SIZE )


   These are allways added (+) to the penalty:
   (if the thermodynamic approach is used then the part in italic
       is substituted with text below this calculation):
       
     + PRIMER_PAIR_WT_DIFF_TM * 
          <difference in Tm between the left and the right primer>

  + PRIMER_PAIR_WT_COMPL_ANY * PRIMER_PAIR_4_COMPL_ANY

     + PRIMER_PAIR_WT_COMPL_END * PRIMER_PAIR_4_COMPL_END

     + PRIMER_PAIR_WT_LIBRARY_MISPRIMING * PRIMER_PAIR_4_LIBRARY_MISPRIMING

     + PRIMER_PAIR_WT_TEMPLATE_MISPRIMING *
          PRIMER_PAIR_4_TEMPLATE_MISPRIMING

     If the thermodynamic approach is used then the part of italic in
     the above calculation is replaced by this:

   If ((min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM) - 5) ≤ PRIMER_PAIR_4_COMPL_ANY_TH) then is added (+):
   + PRIMER_PAIR_WT_COMPL_ANY_TH *
       (PRIMER_PAIR_4_COMPL_ANY_TH - (min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM) - 5 - 1))
  else if ((min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM) - 5) > PRIMER_PAIR_4_COMPL_ANY_TH) then is added (+):
   + PRIMER_PAIR_WT_COMPL_ANY_TH *
      (1/(min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM - 5 + 1 - PRIMER_PAIR_4_COMPL_ANY_TH));
 
   If ((min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM - 5) ≤ PRIMER_PAIR_4_COMPL_END_TH) then is added (+):
   + PRIMER_PAIR_WT_COMPL_END_TH *
       (PRIMER_PAIR_4_COMPL_END_TH - (min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM) - 5 - 1))
  else if ((min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM - 5) > PRIMER_PAIR_4_COMPL_END_TH) then is added (+):
    + PRIMER_PAIR_WT_COMPL_END_TH *
         (1/(min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM - 5 + 1 - PRIMER_PAIR_4_COMPL_ANY_TH));

  If ((min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM - 5) ≤ PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH) then is added (+):
   + PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH *
    (PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH - (min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM - 5 - 1))
  else if ((min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM - 5) > PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH) then is added (+):
   + PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH *
     (1/(min(PRIMER_LEFT_4_TM,PRIMER_RIGHT_4_TM - 5 + 1 - PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH));

Primer3 tries to select pairs with the lowest penalty which still fulfill all necessary requirements like non-redundancy or product size limits.

20. PRIMER3 SETTINGS FILE FORMAT

Primer3 can read global settings from a text file at program start up. This allows the user to save and exchange settings tailored to special applications.

Such a primer3 settings file is a text file. The first three lines of the file have to be as described below followed by tags as they would be provided at standard input:

Primer3 File - http://primer3.sourceforge.net
P3_FILE_TYPE=settings

P3_FILE_ID=Description of the settings
SEQUENCE_TEMPLATE=ATG...
...
...
...
=

In the first line "Primer3 File - http://primer3.sourceforge.net" without tailing space. In the second line "P3_FILE_TYPE=settings". Valid values for P3_FILE_TYPE are all_parameters, sequence and settings. Up to now, only settings is supported. The third line has to be empty. It is strongly advised to describe the settings using the P3_FILE_ID tag. It will print the description of the settings on the output. From the fourth line on regular Boulder-IO can be used as it is used in regular input. It also has to be terminated with a single "=". There can be only one input per file.

21. OUTPUT TAGS

For each Boulder-IO record passed into primer3 via stdin, exactly one Boulder-IO record comes out of primer3 on stdout. If a settings file is provided and the option to echo the settings file is given on the command line, then the contents of the settings file will also be part of the output. Two additional tags are used to indicate where the records of the settings file begin and end: P3_SETTINGS_FILE_USED specifies the path to the settings file that was provided, P3_SETTINGS_FILE_END does not have any value and it just indicates the end of the settings records.

The output records contain everything that the input record contains, plus a subset of the following tag/value pairs. Unless noted by (*), each tag appears for each primer pair returned.

Tags are of the form PRIMER_{LEFT,RIGHT,INTERNAL,PAIR}_<j>_<tag_name> where <j> is an integer from 0 to n, where n is at most the value of PRIMER_NUM_RETURN. In the documentation the output number 4 is shown as for example: PRIMER_LEFT_4_TM.

In the descriptions below, 'i,n' represents a start/length pair, 's' represents a string, x represents an arbitrary integer, and f represents a float.

PRIMER_ERROR=s (*)

s describes user-correctable errors detected in the input (separated by semicolons). This tag is absent if there are no errors.

PRIMER_WARNING=s (*)

s lists warnings generated by primer3` (separated by semicolons); this tag is absent if there are no warnings.

PRIMER_LEFT_NUM_RETURNED=i

PRIMER_RIGHT_NUM_RETURNED=i

PRIMER_INTERNAL_NUM_RETURNED=i

PRIMER_PAIR_NUM_RETURNED=i

i is the number of primers or primer pairs returned on standard output. These tags are always generated under IO version 4 if there are no internal errors and if PRIMER_ERROR is not present.

If primer pairs were requested, PRIMER_LEFT_NUM_RETURNED and PRIMER_RIGHT_NUM_RETURNED will be equal to the number of pairs returned, even if the actual number of distinct left or right primers was lower than the number of pairs. If primer pairs with internal oligos were requested, PRIMER_INTERNAL_NUM_RETURNED will also be set to the number of pairs returned.

If only left or right primers or hybridization (internal) oligos were requested, PRIMER_PAIR_NUM_RETURNED will be 0 and only the relevant tag will have a non-zero value. For example, if only left primers were requested, PRIMER_RIGHT_NUM_RETURNED, PRIMER_INTERNAL_NUM_RETURNED and PRIMER_PAIR_NUM_RETURNED will be 0.

Some tasks, such as pick_sequencing_primers or pick_primer_list, return left and right primers that are not parts of primer pairs. In this case PRIMER_PAIR_NUM_RETURNED will be 0.

PRIMER_LEFT_4_PROBLEMS=s (*)

PRIMER_INTERNAL_4_PROBLEMS=s (*)

PRIMER_RIGHT_4_PROBLEMS=s (*)

s lists the problems (constraint violations) associated with the corresponding primer oligo.

PRIMER_LEFT_EXPLAIN=s (*)

PRIMER_INTERNAL_EXPLAIN=s (*)

PRIMER_RIGHT_EXPLAIN=s (*)

s is a (more or less) self-documenting string containing statistics on the possibilities that primer3 considered in selecting a single oligo. For example

PRIMER_LEFT_EXPLAIN=considered 62, too many Ns 53, ok 9
PRIMER_RIGHT_EXPLAIN=considered 62, too many Ns 53, ok 9
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 87, too many Ns 39, overlap excluded region 40, ok 8

All the categories are exclusive, except the 'considered' category. In some cases the ok count may be higher than the actual number of ok oligos. This is because a primer can be considered as part of pair before all of the primer's characteristics have been computed and checked. If a primer is never in a legal pair or never in a pair with a fully evaluated penalty, then this may occur. This situation never results in a primer pair that contains an illegal primer.

PRIMER_PAIR_EXPLAIN=s (*)

s is a self-documenting string containing statistics on picking a primer pair (plus internal oligo if requested). For example

PRIMER_PAIR_EXPLAIN=considered 81, unacceptable product size 49, no internal oligo 32, ok 0
The purpose of this string is to provide information in the case that not enough primer pairs are returned. This information can be used, for example, to decide which constraints to relax. In some cases the information in this string can also give insight into the causes of long running time. The counts in the string are only approximate, because of several reasons:
  • When there are multiple PRIMER_PRODUCT_SIZE_RANGEs, a primer pair may not be ok in one size range but ok in another. Approximately, the counts for each statistic are summed over all product size ranges.
  • When PRIMER_MIN_THREE_PRIME_DISTANCE is > -1, the number of ok primer pairs reported may be larger than the actual number. The discrepancy is due to a primer in the pair that would overlap a primer in a 'better' (lower penalty) pair. (The search algorithm does not record state that would allow it to detect when a pair that was formerly ok becomes not ok as the result of another primer being inserted into the output list before it is.)
  • In some instances, primer3 will examine a primer pair before it discovers that one of the individual primers in the pair violates specified constraints. In this case PRIMER_PAIR_EXPLAIN might have a non-0 number 'considered', even though one or more of PRIMER_LEFT_EXPLAIN, PRIMER_RIGHT_EXPLAIN, or PRIMER_INTERNAL_EXPLAIN has 'ok 0'.

PRIMER_LEFT_4=i,n

The selected left primer (the primer to the left in the input sequence). i is the 0-based index of the start base of the primer, and n is t its length.

PRIMER_INTERNAL_4=i,n

The selected internal oligo. Primer3 outputs this tag if PRIMER_PICK_INTERNAL_OLIGO was non-0. If primer3 fails to pick a middle oligo upon request, this tag will not be output. i is the 0-based index of start base of the internal oligo, and n is its length.

PRIMER_RIGHT_4=i,n

The selected right primer (the primer to the right in the input sequence). i is the 0-based index of the last base of the primer, and n is its length.

PRIMER_LEFT_4_SEQUENCE=s

PRIMER_INTERNAL_4_SEQUENCE=s

PRIMER_RIGHT_4_SEQUENCE=s

The actual sequence of the oligo. The sequence of left primer and internal oligo is presented 5' -> 3' on the same strand as the input SEQUENCE_TEMPLATE (which must be presented 5' -> 3'). The sequence of the right primer is presented 5' -> 3' on the opposite strand from the input SEQUENCE_TEMPLATE.

PRIMER_PAIR_4_PRODUCT_SIZE=x

x is the product size of the PCR product.

PRIMER_LEFT_4_PENALTY=f

PRIMER_INTERNAL_4_PENALTY=f

PRIMER_RIGHT_4_PENALTY=f

The contribution of this individual primer or oligo to the objective function.

PRIMER_PAIR_4_PENALTY=f

The value of the objective function for this pair (lower is better).

PRIMER_LEFT_4_TM=f

PRIMER_INTERNAL_4_TM=f

PRIMER_RIGHT_4_TM=f

The melting TM for the selected oligo.

PRIMER_PAIR_4_PRODUCT_TM=f

f is the melting temperature of the product. Calculated using equation (iii) from the paper [Rychlik W, Spencer WJ and Rhoads RE (1990) "Optimization of the annealing temperature for DNA amplification in vitro", Nucleic Acids Res 18:6409-12 http://dx.doi.org/10.1093/nar/18.21.6409]. Printed only if a non-default value of PRIMER_PRODUCT_MAX_TM or PRIMER_PRODUCT_MIN_TM is specified.

PRIMER_PAIR_4_PRODUCT_TM_OLIGO_TM_DIFF=f

f is the difference between the melting temperature of the product and the melting temperature of the less stable primer. Printed only if PRIMER_PRODUCT_MAX_TM or PRIMER_PRODUCT_MIN_TM is specified.

PRIMER_PAIR_4_T_OPT_A=f

f is T sub a super OPT from equation (i) in [Rychlik W, Spencer WJ and Rhoads RE (1990) "Optimization of the annealing temperature for DNA amplification in vitro", Nucleic Acids Res 18:6409-12. http://dx.doi.org/10.1093/nar/18.21.6409]. Printed only if PRIMER_PRODUCT_MAX_TM or PRIMER_PRODUCT_MIN_TM is specified.

PRIMER_LEFT_4_GC_PERCENT=f

PRIMER_INTERNAL_4_GC_PERCENT=f

PRIMER_RIGHT_4_GC_PERCENT=f

The percent GC for the selected oligo (denominator is the number of non-ambiguous bases).

PRIMER_LEFT_4_SELF_ANY=f

PRIMER_INTERNAL_4_SELF_ANY=f

PRIMER_RIGHT_4_SELF_ANY=f

The calculated value for the tendency of a primer to bind to itself (interfering with target sequence binding). It will score ANY binding occurring within the entire primer sequence. For details see PRIMER_MAX_SELF_ANY.
The self-complementarity measures for the selected oligo.

PRIMER_LEFT_4_SELF_ANY_TH=f

PRIMER_INTERNAL_4_SELF_ANY_TH=f

PRIMER_RIGHT_4_SELF_ANY_TH=f

The calculated value for the tendency of a primer to bind to itself (interfering with target sequence binding). It will calculate the melting temperature for ANY binding occurring within the entire primer sequence. For details see PRIMER_MAX_SELF_ANY_TH. The self-complementarity measures for the selected oligo.

PRIMER_RIGHT_4_HAIRPIN_TH=f

The calculated value of melting temperature of hairpin structure of primer. For details see PRIMER_MAX_HAIRPIN_TH

PRIMER_PAIR_4_COMPL_ANY=f

The calculated value for the tendency of a primer pair to bind to each other (interfering with target sequence binding). It will score ANY binding occurring within the entire primer sequence. For details see PRIMER_MAX_SELF_ANY.
The inter-pair complementarity measures over the complete primer for selected left and right primer.

PRIMER_PAIR_4_COMPL_ANY_TH=f

The calculated value for the tendency of a primer pair to bind to each other (interfering with target sequence binding). It will calculate the melting temperature of ANY binding occurring within the entire primer sequence. For details see PRIMER_MAX_SELF_ANY_TH. The inter-pair complementarity measures over the complete primer for selected left and right primer.

PRIMER_LEFT_4_SELF_END=f

PRIMER_INTERNAL_4_SELF_END=f

PRIMER_RIGHT_4_SELF_END=f

The calculated value for the tendency of the 3'-END to bind to a identical primer. This is critical for primer quality because it allows primers use itself as a target and amplify a short piece (forming a primer-dimer). These primer are then unable to bind and amplify the target sequence. For details see PRIMER_MAX_SELF_END.
The self-complementarity measures for the ends of selected oligo.

PRIMER_LEFT_4_SELF_END_TH=f

PRIMER_INTERNAL_4_SELF_END_TH=f

PRIMER_RIGHT_4_SELF_END_TH=f

The calculated value for the tendency of the 3'-END to bind to a identical primer. This is critical for primer quality because it allows primers use itself as a target and amplify a short piece (forming a primer-dimer). These primer are then unable to bind and amplify the target sequence. For details see PRIMER_MAX_SELF_END_TH. The self-complementarity measures for the ends of selected oligo.

PRIMER_LEFT_4_HAIRPIN_TH=f

The calculated value of melting temperature of hairpin structure of primer. For details see PRIMER_MAX_HAIRPIN_TH

PRIMER_INTERNAL_4_HAIRPIN_TH=f

The calculated value of melting temperature of hairpin structure of primer. For details see PRIMER_MAX_HAIRPIN_TH

PRIMER_PAIR_4_COMPL_END=f

The calculated value for the tendency of the 3'-ENDs of a primer pair to bind to each other. This is critical for primer quality because it allows primers use itself as a target and amplify a short piece (forming a primer-dimer). These primer are then unable to bind and amplify the target sequence. For details see PRIMER_MAX_SELF_END.
The inter-pair complementarity measures for the ends of selected left and right primer.

PRIMER_PAIR_4_COMPL_END_TH=f

The calculated value for the tendency of the 3'-ENDs of a primer pair to bind to each other. This is critical for primer quality because it allows primers use itself as a target and amplify a short piece (forming a primer-dimer). These primer are then unable to bind and amplify the target sequence. For details see PRIMER_MAX_SELF_END_TH. The inter-pair complementarity measures for the ends of selected left and right primer.

PRIMER_LEFT_4_END_STABILITY=f

PRIMER_RIGHT_4_END_STABILITY=f

f is the delta G of disruption of the five 3' bases of the primer.

PRIMER_LEFT_4_TEMPLATE_MISPRIMING=f

PRIMER_RIGHT_4_TEMPLATE_MISPRIMING=f

PRIMER_PAIR_4_TEMPLATE_MISPRIMING=f

Analogous to PRIMER_{LEFT,RIGHT,PAIR}_LIBRARY_MISPRIMING, except that these output tags apply to mispriming within the template sequence. This often arises, for example, in genes with repeated exons. For backward compatibility, these tags only appear if the corresponding input tags have defined values.

PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=f

PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=f

PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=f

These output tags apply to mispriming within the template sequence and the calculation method is based on thermodynamical approach. This often arises, for example, in genes with repeated exons.

PRIMER_LEFT_4_LIBRARY_MISPRIMING=f, s

PRIMER_RIGHT_4_LIBRARY_MISPRIMING=f, s

PRIMER_PAIR_4_LIBRARY_MISPRIMING=f, s

f is the maximum mispriming score for the right primer against any sequence in the given PRIMER_MISPRIMING_LIBRARY; s is the id of corresponding library sequence. PRIMER_PAIR_MAX_LIBRARY_MISPRIMING is the maximum sum of mispriming scores in any single library sequence (perhaps a more reasonable estimator of the likelihood of mispriming).

PRIMER_INTERNAL_4_LIBRARY_MISHYB=f, s

f is the maximum mishybridization score for the right primer against any sequence in the given PRIMER_INTERNAL_MISHYB_LIBRARY; s is the id of corresponding library sequence.

PRIMER_LEFT_4_MIN_SEQ_QUALITY=i

PRIMER_INTERNAL_4_MIN_SEQ_QUALITY=i

PRIMER_RIGHT_4_MIN_SEQ_QUALITY=i

i is the minimum _sequence_ quality within the primer or oligo (not to be confused with the PRIMER_PAIR_4_PENALTY output tag, which is really the value of the objective function.)

PRIMER_STOP_CODON_POSITION=i

i is the position of the first base of the stop codon, if primer3 found one, or -1 if primer3 did not. Printed only if the input tag SEQUENCE_START_CODON_POSITION with a non-default value is supplied.

PRIMER_LEFT_4_POSITION_PENALTY=i

PRIMER_RIGHT_4_POSITION_PENALTY=i

i is the penalty of the primer by its position.

22. EXAMPLE OUTPUT

You should run it yourself. Use the file 'example' that came with this distribution directory as input.

23. ADVICE FOR PICKING PRIMERS

We suggest consulting: Wojciech Rychlik (1993) "Selection of Primers for Polymerase Chain Reaction" in BA White, Ed., "Methods in Molecular Biology, Vol. 15: PCR Protocols: Current Methods and Applications", pp 31-40, Humana Press, Totowa NJ.

24. CAUTIONS

Some of the most important issues in primer picking can be addressed only before using primer3. These are sequence quality (including making sure the sequence is not vector and not chimeric) and avoiding repetitive elements.

Techniques for avoiding problems include a thorough understanding of possible vector contaminants and cloning artifacts coupled with database searches using blast, Fasta, or other similarity searching program to screen for vector contaminants and possible repeats. Repbase (J. Jurka, A.F.A. Smit, C. Pethiyagoda, and others, 1995-1996, ftp://ftp.ncbi.nih.gov/repository/repbase/) is an excellent source of repeat sequences and pointers to the literature. (The Repbase files need to be converted to Fasta format before they can be used by primer3.) Primer3 now allows you to screen candidate oligos against a Mispriming Library (or a Mishyb Library in the case of internal oligos).

Sequence quality can be controlled by manual trace viewing and quality clipping or automatic quality clipping programs. Low- quality bases should be changed to N's or can be made part of Excluded Regions. The beginning of a sequencing read is often problematic because of primer peaks, and the end of the read often contains many low-quality or even meaningless called bases. Therefore, when picking primers from single-pass sequence it is often best to use the SEQUENCE_INCLUDED_REGION parameter to ensure that primer3 chooses primers in the high quality region of the read.

In addition, primer3 takes as input a SEQUENCE_QUALITY list for use with those base calling programs (e.g. Phred) that output this information.

25. WHAT TO DO IF PRIMER3 CANNOT FIND ANY PRIMERS?

Try relaxing various parameters, including the self-complementarity parameters and max and min oligo melting temperatures. For example, for very A-T-rich regions you might have to increase maximum primer size or decrease minimum melting temperature. It is usually unwise to reduce the minimum primer size if your template is complex (e.g. a mammalian genome), since small primers are more likely to be non-specific. Make sure that there are adequate stretches of non-Ns in the regions in which you wish to pick primers. If necessary you can also allow an N in your primer and use an oligo mixture containing all four bases at that position.

Try setting the PRIMER_EXPLAIN_FLAG input tag.

26. DIFFERENCES FROM EARLIER VERSIONS

The section HOW TO MIGRATE TAGS TO IO VERSION 4 describes th modified tags in detail. See also the file release_notes.txt in this directory.

27. EXIT STATUS CODES

 0 on normal operation
-1 under the following conditions:
   illegal command-line arguments.
   unable to fflush stdout.
   unable to open (for writing and creating) a .for, .rev
     or .int file (probably due to a protection problem).
-2 on out-of-memory
-3 empty input
-4 error in a "Global" input tag (message in PRIMER_ERROR).

Primer3 calls abort() and dumps core (if possible) if a programming error is detected by an assertion violation.

SIGINT and SIGTERM are handled essentially as empty input, except the signal received is returned as the exit status and printed to stderr.

In all of the error cases above Primer3 prints a message to stderr.

28. PRIMER3 WWW INTERFACES

There are two web interfaces available :
The Whitehead Institute for Biomedical Research provides a basic web-based front end to primer3 named primer3web at http://primer3.wi.mit.edu/

A Primer3plus web services is at http://primer3plus.com

This distribution does not contain the Primer3 WWW interfaces.

Web interface code is available at http://sourceforge.net/projects/primer3/.

29. ACKNOWLEDGMENTS

Initial development of Primer3 was funded by Howard Hughes Medical Institute and by the National Institutes of Health, National Human Genome Research Institute under grants R01-HG00257 (to David C. Page) and P50-HG00098 (to Eric S. Lander), but ongoing development and maintenance are not currently funded.

Primer3 was originally written by Helen J. Skaletsky (Howard Hughes Medical Institute, Whitehead Institute) and Steve Rozen (Duke-NUS Graduate Medical School Singapore, formerly at Whitehead Institute) based on the design of earlier versions, notably Primer 0.5 (Steve Lincoln, Mark Daly, and Eric S. Lander). The original web interface was designed by Richard Resnick. Lincoln Stein designed the Boulder-IO format in the days before XML and RDF, and championed the idea of making primer3 a software component, which has been key to its wide utility.

In addition, among others, Ernst Molitor, Carl Foeller, and James Bonfield contributed to the early design of primer3. Brant Faircloth has helped with ensuring that primer3 runs on Windows and MacOS and with the primer3 web site. Triinu Koressaar and Maido Remm modernized the melting temperature calculations in 2008. Triinu Koressaar added secondary structure, primer-dimer, and template mispriming based on a thermodynamic model in 2.2.0. Ioana Cutcutache is responsible for most of the remaining improvements in 2.2.0, including performance enhancements, modern command line arguments, and new input tags to control primer location (with the "overlap junction" tags initially implemented by Andreas Untergasser). Jian Ye patiently provided new requirements.

Harm Nijveen and Andreas Untergasser developed the webinterface Primer3Plus in 2006-2009. Currently Primer3Plus is maintained by Andreas Untergasser.

Primer3 is an open software development project hosted on SourceForge: http://sourceforge.net/projects/primer3/.

primer3-2.3.6/test/0000775000077300007730000000000012231156365013040 5ustar ioanaioanaprimer3-2.3.6/test/th-w-other-tasks_list_tmp/0000775000077300007730000000000012231156372020072 5ustar ioanaioanaprimer3-2.3.6/test/thal_input0000775000077300007730000005203512231156245015137 0ustar ioanaioana-s ccgcagtaagctgcgg -a HAIRPIN -s1 ccgcagtaagctgcgg -a HAIRPIN -s2 ccgcagtaagctgcgg -a HAIRPIN -s2 ccgcagtaagctgcgg -a HAIRPIN -maxloop -s1 ccgcagtaagctgcgg -s2 ccgcagtaagctgcgg -a ANY -maxloop -1 -s1 ccgcagtaagctgcgg -s2 ccgcagtaagctgcgg -a ANY -maxloop 0 -s1 ccgcagtaagctgcgg -s2 ccgcagtaagctgcgg -a ANY -maxloop 1 -s1 ccgcagtaagctgcgg -s2 ccgcagtaagctgcgg -dv -s1 ccgcagtaagctgcgg -s2 ccgcagtaagctgcgg -mv -s1 ccgcagtaagctgcgg -s2 ccgcagtaagctgcgg -n -s1 ccgcagtaagctgcgg -s2 ccgcagtaagctgcgg -d -s1 ccgcagtaagctgcgg -s2 ccgcagtaagctgcgg -t -s1 ccgcagtaagctgcgg -s2 ccgcagtaagctgcgg -dv -1 -s1 ccgcagtaagctgcgg -s2 ccgcagtaagctgcgg -mv -1 -s1 ccgcagtaagctgcgg -s2 ccgcagtaagctgcgg -n -1 -s1 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TAAAAAAAAAAAAAA -s2 GGGGGGTGGGGGGGG -a END2 -maxloop 3 -s1 TAAAAAAAAAAAAAA -a HAIRPIN -maxloop 3 -s2 GGGGGGTGGGGGGGG -a HAIRPIN -maxloop 1 -s1 AAAACCCGCTTTGCTAGCTACG -s2 AACCCGCTTGCTAGCAAAAAAAA -a ANY -maxloop 1 -s1 AAAACCCGCTTTGCTAGCTACG -s2 AACCCGCTTGCTAGCAAAAAAAA -a END1 -maxloop 1 -s1 AAAACCCGCTTTGCTAGCTACG -s2 AACCCGCTTGCTAGCAAAAAAAA -a END2 -maxloop 1 -s1 AAAACCCGCTTTGCTAGCTACG -a HAIRPIN -maxloop 1 -s2 AACCCGCTTGCTAGCAAAAAAAA -a HAIRPIN -maxloop 1 -s1 AAACACACACACACACT -s2 TGGGGGTGTTGGGGTTGTGG -a ANY -maxloop 1 -s1 AAACACACACACACACT -s2 TGGGGGTGTTGGGGTTGTGG -a END1 -maxloop 1 -s1 AAACACACACACACACT -s2 TGGGGGTGTTGGGGTTGTGG -a END2 -maxloop 1 -s1 AAACACACACACACACT -a HAIRPIN -maxloop 1 -s2 TGGGGGTGTTGGGGTTGTGG -a HAIRPIN -maxloop 1 -s1 AAACGATCGTAGCGTTTCAGTCT -s2 AGTCTCTCTCTCTCTCTCTC -a ANY -maxloop 1 -s1 AAACGATCGTAGCGTTTCAGTCT -s2 AGTCTCTCTCTCTCTCTCTC -a END1 -maxloop 1 -s1 AAACGATCGTAGCGTTTCAGTCT -s2 AGTCTCTCTCTCTCTCTCTC -a END2 -maxloop 1 -s1 AAACGATCGTAGCGTTTCAGTCT -a HAIRPIN -maxloop 1 -s2 AGTCTCTCTCTCTCTCTCTC -a HAIRPIN -maxloop 1 -s1 ACGCCGTTGCGCGCTGCTGCG -s2 TTTTTTTTTTTTTTTTTTA -a ANY -maxloop 1 -s1 ACGCCGTTGCGCGCTGCTGCG -s2 TTTTTTTTTTTTTTTTTTA -a END1 -maxloop 1 -s1 ACGCCGTTGCGCGCTGCTGCG -s2 TTTTTTTTTTTTTTTTTTA -a END2 -maxloop 1 -s1 ACGCCGTTGCGCGCTGCTGCG -a HAIRPIN -maxloop 1 -s2 TTTTTTTTTTTTTTTTTTA -a HAIRPIN -maxloop 1 -s1 TTTTTTTTTTATTTTTTTTT -s2 GGGGGGGGAGGGGGGGGG -a ANY -maxloop 1 -s1 TTTTTTTTTTATTTTTTTTT -s2 GGGGGGGGAGGGGGGGGG -a END1 -maxloop 1 -s1 TTTTTTTTTTATTTTTTTTT -s2 GGGGGGGGAGGGGGGGGG -a END2 -maxloop 1 -s1 TTTTTTTTTTATTTTTTTTT -a HAIRPIN -maxloop 1 -s2 GGGGGGGGAGGGGGGGGG -a HAIRPIN -maxloop 1 -s1 AACCGGGTCTTTTTTTAACC -s2 AACCTTAAAAAAAAACCGG -a ANY -maxloop 1 -s1 AACCGGGTCTTTTTTTAACC -s2 AACCTTAAAAAAAAACCGG -a END1 -maxloop 1 -s1 AACCGGGTCTTTTTTTAACC -s2 AACCTTAAAAAAAAACCGG -a END2 -maxloop 1 -s1 AACCGGGTCTTTTTTTAACC -a HAIRPIN -maxloop 1 -s2 AACCTTAAAAAAAAACCGG -a HAIRPIN -maxloop 1 -s1 AAACGATCGATTTT -s2 AAACGATCGACCCC -a ANY -maxloop 1 -s1 AAACGATCGATTTT -s2 AAACGATCGACCCC -a END1 -maxloop 1 -s1 AAACGATCGATTTT -s2 AAACGATCGACCCC -a END2 -maxloop 1 -s1 AAACGATCGATTTT -a HAIRPIN -maxloop 1 -s2 AAACGATCGACCCC -a HAIRPIN -maxloop 1 -s1 AAAAAAAAAAAAAAAT -s2 CCCCCCCCCCCCCT -a ANY -maxloop 1 -s1 AAAAAAAAAAAAAAAT -s2 CCCCCCCCCCCCCT -a END1 -maxloop 1 -s1 AAAAAAAAAAAAAAAT -s2 CCCCCCCCCCCCCT -a END2 -maxloop 1 -s1 AAAAAAAAAAAAAAAT -a HAIRPIN -maxloop 1 -s2 CCCCCCCCCCCCCT -a HAIRPIN -maxloop 1 -s1 AAAAAAAAAAAAAAAT -s2 TCCCCCCCCCCCCC -a ANY -maxloop 1 -s1 AAAAAAAAAAAAAAAT -s2 TCCCCCCCCCCCCC -a END1 -maxloop 1 -s1 AAAAAAAAAAAAAAAT -s2 TCCCCCCCCCCCCC -a END2 -maxloop 1 -s1 AAAAAAAAAAAAAAAT -a HAIRPIN -maxloop 1 -s2 TCCCCCCCCCCCCC -a HAIRPIN -maxloop 1 -s1 ACACACACACACACA -s2 TGTGTTGGTGTTGTTGTGTG -a ANY -maxloop 1 -s1 ACACACACACACACA -s2 TGTGTTGGTGTTGTTGTGTG -a END1 -maxloop 1 -s1 ACACACACACACACA -s2 TGTGTTGGTGTTGTTGTGTG -a END2 -maxloop 1 -s1 ACACACACACACACA -a HAIRPIN -maxloop 1 -s2 TGTGTTGGTGTTGTTGTGTG -a HAIRPIN -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGTGGGGGGGGG -a ANY -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGTGGGGGGGGG -a END1 -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGTGGGGGGGGG -a END2 -maxloop 1 -s1 TAAAAAAAAAAAAAA -a HAIRPIN -maxloop 1 -s2 GGGGGGGTGGGGGGGGG -a HAIRPIN -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGGGGGGGGT -a ANY -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGGGGGGGGT -a END1 -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGGGGGGGGT -a END2 -maxloop 1 -s1 TAAAAAAAAAAAAAA -a HAIRPIN -maxloop 1 -s2 GGGGGGGGGGGGGGT -a HAIRPIN -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 TGGGGGGGGGGGGGGG -a ANY -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 TGGGGGGGGGGGGGGG -a END1 -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 TGGGGGGGGGGGGGGG -a END2 -maxloop 1 -s1 TAAAAAAAAAAAAAA -a HAIRPIN -maxloop 1 -s2 TGGGGGGGGGGGGGGG -a HAIRPIN -maxloop 1 -s1 AAAAAAATAAAAAAA -s2 TGGGGGGGGGGGGGGG -a ANY -maxloop 1 -s1 AAAAAAATAAAAAAA -s2 TGGGGGGGGGGGGGGG -a END1 -maxloop 1 -s1 AAAAAAATAAAAAAA -s2 TGGGGGGGGGGGGGGG -a END2 -maxloop 1 -s1 AAAAAAATAAAAAAA -a HAIRPIN -maxloop 1 -s2 TGGGGGGGGGGGGGGG -a HAIRPIN -maxloop 1 -s1 AAAAAAATAAAAAAA -s2 GGGGGGGGGGGGGGT -a ANY -maxloop 1 -s1 AAAAAAATAAAAAAA -s2 GGGGGGGGGGGGGGT -a END1 -maxloop 1 -s1 AAAAAAATAAAAAAA -s2 GGGGGGGGGGGGGGT -a END2 -maxloop 1 -s1 AAAAAAATAAAAAAA -a HAIRPIN -maxloop 1 -s2 GGGGGGGGGGGGGGT -a HAIRPIN -maxloop 1 -s1 AAAAAAAAAAAAAAT -s2 GGGGGGTGGGGGGGG -a ANY -maxloop 1 -s1 AAAAAAAAAAAAAAT -s2 GGGGGGTGGGGGGGG -a END1 -maxloop 1 -s1 AAAAAAAAAAAAAAT -s2 GGGGGGTGGGGGGGG -a END2 -maxloop 1 -s1 AAAAAAAAAAAAAAT -a HAIRPIN -maxloop 1 -s2 GGGGGGTGGGGGGGG -a HAIRPIN -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGTGGGGGGGG -a ANY -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGTGGGGGGGG -a END1 -maxloop 1 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGTGGGGGGGG -a END2 -maxloop 1 -s1 TAAAAAAAAAAAAAA -a HAIRPIN -maxloop 1 -s2 GGGGGGTGGGGGGGG -a HAIRPIN -maxloop 0 -s1 AAAACCCGCTTTGCTAGCTACG -s2 AACCCGCTTGCTAGCAAAAAAAA -a ANY -maxloop 0 -s1 AAAACCCGCTTTGCTAGCTACG -s2 AACCCGCTTGCTAGCAAAAAAAA -a END1 -maxloop 0 -s1 AAAACCCGCTTTGCTAGCTACG -s2 AACCCGCTTGCTAGCAAAAAAAA -a END2 -s1 AAAACCCGCTTTGCTAGCTACG -a HAIRPIN -s2 AACCCGCTTGCTAGCAAAAAAAA -a HAIRPIN -maxloop 0 -s1 AAACACACACACACACT -s2 TGGGGGTGTTGGGGTTGTGG -a ANY -maxloop 0 -s1 AAACACACACACACACT -s2 TGGGGGTGTTGGGGTTGTGG -a END1 -maxloop 0 -s1 AAACACACACACACACT -s2 TGGGGGTGTTGGGGTTGTGG -a END2 -maxloop 0 -s1 AAACACACACACACACT -a HAIRPIN -maxloop 0 -s2 TGGGGGTGTTGGGGTTGTGG -a HAIRPIN -maxloop 0 -s1 AAACGATCGTAGCGTTTCAGTCT -s2 AGTCTCTCTCTCTCTCTCTC -a ANY -maxloop 0 -s1 AAACGATCGTAGCGTTTCAGTCT -s2 AGTCTCTCTCTCTCTCTCTC -a END1 -maxloop 0 -s1 AAACGATCGTAGCGTTTCAGTCT -s2 AGTCTCTCTCTCTCTCTCTC -a END2 -maxloop 0 -s1 AAACGATCGTAGCGTTTCAGTCT -a HAIRPIN -maxloop 0 -s2 AGTCTCTCTCTCTCTCTCTC -a HAIRPIN -maxloop 0 -s1 ACGCCGTTGCGCGCTGCTGCG -s2 TTTTTTTTTTTTTTTTTTA -a ANY -maxloop 0 -s1 ACGCCGTTGCGCGCTGCTGCG -s2 TTTTTTTTTTTTTTTTTTA -a END1 -maxloop 0 -s1 ACGCCGTTGCGCGCTGCTGCG -s2 TTTTTTTTTTTTTTTTTTA -a END2 -maxloop 0 -s1 ACGCCGTTGCGCGCTGCTGCG -a HAIRPIN -maxloop 0 -s2 TTTTTTTTTTTTTTTTTTA -a HAIRPIN -maxloop 0 -s1 TTTTTTTTTTATTTTTTTTT -s2 GGGGGGGGAGGGGGGGGG -a ANY -maxloop 0 -s1 TTTTTTTTTTATTTTTTTTT -s2 GGGGGGGGAGGGGGGGGG -a END1 -maxloop 0 -s1 TTTTTTTTTTATTTTTTTTT -s2 GGGGGGGGAGGGGGGGGG -a END2 -maxloop 0 -s1 TTTTTTTTTTATTTTTTTTT -a HAIRPIN -maxloop 0 -s2 GGGGGGGGAGGGGGGGGG -a HAIRPIN -maxloop 0 -s1 AACCGGGTCTTTTTTTAACC -s2 AACCTTAAAAAAAAACCGG -a ANY -maxloop 0 -s1 AACCGGGTCTTTTTTTAACC -s2 AACCTTAAAAAAAAACCGG -a END1 -maxloop 0 -s1 AACCGGGTCTTTTTTTAACC -s2 AACCTTAAAAAAAAACCGG -a END2 -maxloop 0 -s1 AACCGGGTCTTTTTTTAACC -a HAIRPIN -maxloop 0 -s2 AACCTTAAAAAAAAACCGG -a HAIRPIN -maxloop 0 -s1 AAACGATCGATTTT -s2 AAACGATCGACCCC -a ANY -maxloop 0 -s1 AAACGATCGATTTT -s2 AAACGATCGACCCC -a END1 -maxloop 0 -s1 AAACGATCGATTTT -s2 AAACGATCGACCCC -a END2 -maxloop 0 -s1 AAACGATCGATTTT -a HAIRPIN -maxloop 0 -s2 AAACGATCGACCCC -a HAIRPIN -maxloop 0 -s1 AAAAAAAAAAAAAAAT -s2 CCCCCCCCCCCCCT -a ANY -maxloop 0 -s1 AAAAAAAAAAAAAAAT -s2 CCCCCCCCCCCCCT -a END1 -maxloop 0 -s1 AAAAAAAAAAAAAAAT -s2 CCCCCCCCCCCCCT -a END2 -maxloop 0 -s1 AAAAAAAAAAAAAAAT -a HAIRPIN -maxloop 0 -s2 CCCCCCCCCCCCCT -a HAIRPIN -maxloop 0 -s1 AAAAAAAAAAAAAAAT -s2 TCCCCCCCCCCCCC -a ANY -maxloop 0 -s1 AAAAAAAAAAAAAAAT -s2 TCCCCCCCCCCCCC -a END1 -maxloop 0 -s1 AAAAAAAAAAAAAAAT -s2 TCCCCCCCCCCCCC -a END2 -maxloop 0 -s1 AAAAAAAAAAAAAAAT -a HAIRPIN -maxloop 0 -s2 TCCCCCCCCCCCCC -a HAIRPIN -maxloop 0 -s1 ACACACACACACACA -s2 TGTGTTGGTGTTGTTGTGTG -a ANY -maxloop 0 -s1 ACACACACACACACA -s2 TGTGTTGGTGTTGTTGTGTG -a END1 -maxloop 0 -s1 ACACACACACACACA -s2 TGTGTTGGTGTTGTTGTGTG -a END2 -maxloop 0 -s1 ACACACACACACACA -a HAIRPIN -maxloop 0 -s2 TGTGTTGGTGTTGTTGTGTG -a HAIRPIN -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGTGGGGGGGGG -a ANY -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGTGGGGGGGGG -a END1 -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGTGGGGGGGGG -a END2 -maxloop 0 -s1 TAAAAAAAAAAAAAA -a HAIRPIN -maxloop 0 -s2 GGGGGGGTGGGGGGGGG -a HAIRPIN -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGGGGGGGGT -a ANY -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGGGGGGGGT -a END1 -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGGGGGGGGGT -a END2 -maxloop 0 -s1 TAAAAAAAAAAAAAA -a HAIRPIN -maxloop 0 -s2 GGGGGGGGGGGGGGT -a HAIRPIN -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 TGGGGGGGGGGGGGGG -a ANY -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 TGGGGGGGGGGGGGGG -a END1 -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 TGGGGGGGGGGGGGGG -a END2 -maxloop 0 -s1 TAAAAAAAAAAAAAA -a HAIRPIN -maxloop 0 -s2 TGGGGGGGGGGGGGGG -a HAIRPIN -maxloop 0 -s1 AAAAAAATAAAAAAA -s2 TGGGGGGGGGGGGGGG -a ANY -maxloop 0 -s1 AAAAAAATAAAAAAA -s2 TGGGGGGGGGGGGGGG -a END1 -maxloop 0 -s1 AAAAAAATAAAAAAA -s2 TGGGGGGGGGGGGGGG -a END2 -maxloop 0 -s1 AAAAAAATAAAAAAA -a HAIRPIN -maxloop 0 -s2 TGGGGGGGGGGGGGGG -a HAIRPIN -maxloop 0 -s1 AAAAAAATAAAAAAA -s2 GGGGGGGGGGGGGGT -a ANY -maxloop 0 -s1 AAAAAAATAAAAAAA -s2 GGGGGGGGGGGGGGT -a END1 -maxloop 0 -s1 AAAAAAATAAAAAAA -s2 GGGGGGGGGGGGGGT -a END2 -maxloop 0 -s1 AAAAAAATAAAAAAA -a HAIRPIN -maxloop 0 -s2 GGGGGGGGGGGGGGT -a HAIRPIN -maxloop 0 -s1 AAAAAAAAAAAAAAT -s2 GGGGGGTGGGGGGGG -a ANY -maxloop 0 -s1 AAAAAAAAAAAAAAT -s2 GGGGGGTGGGGGGGG -a END1 -maxloop 0 -s1 AAAAAAAAAAAAAAT -s2 GGGGGGTGGGGGGGG -a END2 -maxloop 0 -s1 AAAAAAAAAAAAAAT -a HAIRPIN -maxloop 0 -s2 GGGGGGTGGGGGGGG -a HAIRPIN -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGTGGGGGGGG -a ANY -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGTGGGGGGGG -a END1 -maxloop 0 -s1 TAAAAAAAAAAAAAA -s2 GGGGGGTGGGGGGGG -a END2 -maxloop 0 -s1 TAAAAAAAAAAAAAA -a HAIRPIN -maxloop 0 -s2 GGGGGGTGGGGGGGG -a HAIRPIN -s1 AAGATCAGCTAGCTAGCTAGC -s2 TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT -a ANY -s1 AAGATCAGCTAGCTAGCTAGC -s2 TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT -a END1 -s1 AAGATCAGCTAGCTAGCTAGC -s2 TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT -a END2 -s1 AAGATCAGCTAGCTAGCTAGC -a HAIRPIN -s2 TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT -a HAIRPIN -s1 GATCAGCTAGCTTTTT -s2 TTTTTTGATCAGCTAGCTTTTT -a ANY -s1 GATCAGCTAGCTTTTT -s2 TTTTTTGATCAGCTAGCTTTTT -a END1 -s1 GATCAGCTAGCTTTTT -s2 TTTTTTGATCAGCTAGCTTTTT -a END2 -s1 GATCAGCTAGCTTTTT -a HAIRPIN -s2 TTTTTTGATCAGCTAGCTTTTT -a HAIRPIN -s1 AAGATCAGCTAGCTAGCTAGCTTTTT -s2 TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT -a ANY -s1 AAGATCAGCTAGCTAGCTAGCTTTTT -s2 TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT -a END1 -s1 AAGATCAGCTAGCTAGCTAGCTTTTT -s2 TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT -a END2 -s1 AAGATCAGCTAGCTAGCTAGCTTTTT -a HAIRPIN -s2 TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT -a HAIRPIN -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -a ANY -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -a END1 -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -a END2 -s1 CGATATATTTGGTCTATA -a HAIRPIN -s2 CGATATATTTCCGGTCTATA -a HAIRPIN -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -mv 1000 -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -mv 1000 -dv 2.5 -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -mv 50 -dv 2.5 -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -mv 50 -dv 2.5 -n 0.8 -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -d 100 -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -d 1000 -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -mv 1000 -d 1000 -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -t 10 -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -t 37 -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -t 55 -s1 CGATATATTTGGTCTATA -s2 CGATATATTTCCGGTCTATA -t 100 -s1 CGATATATTTGGTCTATA -a HAIRPIN -mv 1000 -dv 2.5 -n 0.8 -s2 CGATATATTTCCGGTCTATA -a HAIRPIN -mv 1000 -dv 2.5 -n 2.5 -s2 CGATATATTTCCGGTCTATA -a HAIRPIN -mv 1000 -dv 0 -n 2.5 -s2 CGATATATTTCCGGTCTATA -a HAIRPIN -mv 1000 -dv 0 -n 0 -s2 CGATATATTTCCGGTCTATA -a HAIRPIN -mv 1000 -dv 2.5 -n 0 -s1 gtgtgcaaaa -s2 ttttgcacc -s1 agtgtagtacgg -s2 caaaaccgtgtc -s1 agtgtagtacgg -s2 caaaaccgtgtc -mv 1000 -s1 agtgtagtacgg -s2 caaaaccgtgtc -maxloop 1 -s1 agtgtagtacgg -s2 caaaaccgtgtc -maxloop 3 -s1 agtgtagtacgg -s2 caaaaccgtgtc -a END1 -s1 agtgtagtacgg -s2 caaaaccgtgtc -a END2 -s1 CCAAAACCCATGATGATGATGGGTTTTGG -a HAIRPIN -s1 CCCCAAAACCCATGATGATGATGGGTTTTGGGG -a HAIRPIN primer3-2.3.6/test/primer_boundary_output0000664000077300007730000036246312231156245017617 0ustar ioanaioanaPRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=0 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=0 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0 PRIMER_SALT_DIVALENT=0.0 PRIMER_DNTP_CONC=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 SEQUENCE_ID=boundary_test_00 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MAX_END_STABILITY=100.0 PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-100 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_02 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_03 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=56-100 P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_04 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=50-54 P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_05 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MIN_SIZE=24 PRIMER_MAX_SIZE=24 PRIMER_OPT_SIZE=24 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.330996 PRIMER_LEFT_0_PENALTY=0.243965 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_LEFT_0=2,24 PRIMER_RIGHT_0=56,24 PRIMER_LEFT_0_TM=60.244 PRIMER_RIGHT_0_TM=59.913 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_06 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MIN_SIZE=26 PRIMER_MAX_SIZE=26 PRIMER_OPT_SIZE=26 P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 5, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_07 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_08 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 SEQUENCE_INCLUDED_REGION=3,55 P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 6, ok 0 PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_09 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=50-100 SEQUENCE_INCLUDED_REGION=2,54 P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 5, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_10 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3 PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=1.826922 PRIMER_LEFT_0_PENALTY=1.177322 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTNATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=61.177 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=9.50 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.75 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_12 PRIMER_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.870774 PRIMER_LEFT_0_PENALTY=3.221174 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCNNATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=63.221 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=34.783 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=11.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_13 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.811551 PRIMER_LEFT_0_PENALTY=4.161950 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=55.838 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=0.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_14 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=.0001 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_15 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=20 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=31.102361 PRIMER_LEFT_0_PENALTY=30.452761 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGGG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=90.453 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=100.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=1.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_16 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=.0001 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_17 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_18 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 1, too many Ns 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 1, too many Ns 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_19 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,54 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=boundary_test_20 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=54-55 SEQUENCE_INCLUDED_REGION=2,54 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, too many Ns 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_21 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_ID=boundary_test_22 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=26,6 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 2, ok 0 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_23 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,6 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 2, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_24 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 2, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,1 SEQUENCE_TARGET=28,4 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_26 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=26,1 SEQUENCE_TARGET=27,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, overlap target 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_27 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,1 SEQUENCE_TARGET=28,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap target 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_28 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,1 SEQUENCE_TARGET=29,4 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap target 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_29 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_30 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,0 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_31 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=0,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_32 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_33 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=26,6 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_34 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,6 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_35 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_36 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,1 SEQUENCE_EXCLUDED_REGION=28,4 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_37 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=26,1 SEQUENCE_EXCLUDED_REGION=27,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_37 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,1 SEQUENCE_EXCLUDED_REGION=28,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_38 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 SEQUENCE_EXCLUDED_REGION=29,4 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_39 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_40 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,0 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_41 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=10,0 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_42 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_43 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=9.99 PRIMER_PAIR_MAX_COMPL_END=9.99 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because one primer has end compl PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, high end compl 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_44 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=10.01 PRIMER_PAIR_MAX_COMPL_END=10.01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_45 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=12.01 PRIMER_PAIR_MAX_COMPL_ANY=12.01 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_46 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=11.99 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_PAIR_MAX_COMPL_ANY=11.99 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because self any for one primer is 12.00" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, high any compl 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_47 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_48 PRIMER_GC_CLAMP=1 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because of GC clamp)" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, GC clamp failed 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_49 PRIMER_GC_CLAMP=1 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=5.752125 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=3.020452 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=63.020 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_50 PRIMER_GC_CLAMP=2 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because of GC clamp)" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC clamp failed 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_51 PRIMER_PAIR_MAX_DIFF_TM=100 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=5.752125 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=3.020452 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=63.020 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_52 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=5.752125 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=3.020452 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=63.020 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_53 PRIMER_PAIR_MAX_DIFF_TM=0.28 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (due to max diff tm)" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, tm diff too large 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_54 PRIMER_LIBERAL_BASE=1 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_WARNING=Unrecognized base in input sequence PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=5.752125 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=3.020452 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=63.020 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_55 PRIMER_LIBERAL_BASE=0 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should generate error" PRIMER_ERROR=Unrecognized base in input sequence = SEQUENCE_ID=poly_x_1 SEQUENCE_TEMPLATE=AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTTCCCCCC PRIMER_MAX_POLY_X=5 PRIMER_INTERNAL_MAX_POLY_X=6 PRIMER_PRODUCT_SIZE_RANGE=50-300 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PAIR_MAX_DIFF_TM=100 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_LEFT_EXPLAIN=considered 138, long poly-x seq 138, ok 0 PRIMER_RIGHT_EXPLAIN=considered 138, long poly-x seq 138, ok 0 PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=poly_x_2 PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 SEQUENCE_TEMPLATE=AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG PRIMER_LEFT_EXPLAIN=considered 131, ok 131 PRIMER_RIGHT_EXPLAIN=considered 131, ok 131 PRIMER_INTERNAL_EXPLAIN=considered 1585, ok 1585 PRIMER_PAIR_EXPLAIN=considered 64, unacceptable product size 48, ok 16 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=15.547212 PRIMER_LEFT_0_PENALTY=7.773606 PRIMER_RIGHT_0_PENALTY=7.773606 PRIMER_INTERNAL_0_PENALTY=1.499588 PRIMER_LEFT_0_SEQUENCE=CTTTTTGGGGGAAAAATTTTTCCCC PRIMER_RIGHT_0_SEQUENCE=GAAAAATTTTTCCCCCAAAAAGGGG PRIMER_INTERNAL_0_SEQUENCE=CCCTTTTTGGGGGAAAAAC PRIMER_LEFT_0=29,25 PRIMER_RIGHT_0=130,25 PRIMER_INTERNAL_0=87,19 PRIMER_LEFT_0_TM=67.774 PRIMER_RIGHT_0_TM=67.774 PRIMER_INTERNAL_0_TM=60.500 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=47.368 PRIMER_LEFT_0_SELF_ANY=18.00 PRIMER_RIGHT_0_SELF_ANY=12.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=18.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=10.9000 PRIMER_PAIR_0_COMPL_ANY=22.00 PRIMER_PAIR_0_COMPL_END=14.00 PRIMER_PAIR_0_PRODUCT_SIZE=102 PRIMER_PAIR_1_PENALTY=15.547212 PRIMER_LEFT_1_PENALTY=7.773606 PRIMER_RIGHT_1_PENALTY=7.773606 PRIMER_INTERNAL_1_PENALTY=1.499588 PRIMER_LEFT_1_SEQUENCE=CTTTTTGGGGGAAAAATTTTTCCCC PRIMER_RIGHT_1_SEQUENCE=GGAAAAATTTTTCCCCCAAAAAGGG PRIMER_INTERNAL_1_SEQUENCE=CCTTTTTGGGGGAAAAACC PRIMER_LEFT_1=29,25 PRIMER_RIGHT_1=131,25 PRIMER_INTERNAL_1=88,19 PRIMER_LEFT_1_TM=67.774 PRIMER_RIGHT_1_TM=67.774 PRIMER_INTERNAL_1_TM=60.500 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=47.368 PRIMER_LEFT_1_SELF_ANY=18.00 PRIMER_RIGHT_1_SELF_ANY=14.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=18.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=10.9000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=23.00 PRIMER_PAIR_1_COMPL_END=19.00 PRIMER_PAIR_1_PRODUCT_SIZE=103 PRIMER_PAIR_2_PENALTY=15.547212 PRIMER_LEFT_2_PENALTY=7.773606 PRIMER_RIGHT_2_PENALTY=7.773606 PRIMER_INTERNAL_2_PENALTY=1.499588 PRIMER_LEFT_2_SEQUENCE=CTTTTTGGGGGAAAAATTTTTCCCC PRIMER_RIGHT_2_SEQUENCE=GGGAAAAATTTTTCCCCCAAAAAGG PRIMER_INTERNAL_2_SEQUENCE=CTTTTTGGGGGAAAAACCC PRIMER_LEFT_2=29,25 PRIMER_RIGHT_2=132,25 PRIMER_INTERNAL_2=89,19 PRIMER_LEFT_2_TM=67.774 PRIMER_RIGHT_2_TM=67.774 PRIMER_INTERNAL_2_TM=60.500 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=47.368 PRIMER_LEFT_2_SELF_ANY=18.00 PRIMER_RIGHT_2_SELF_ANY=16.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=18.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=10.9000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=24.00 PRIMER_PAIR_2_COMPL_END=22.00 PRIMER_PAIR_2_PRODUCT_SIZE=104 PRIMER_PAIR_3_PENALTY=15.547212 PRIMER_LEFT_3_PENALTY=7.773606 PRIMER_RIGHT_3_PENALTY=7.773606 PRIMER_INTERNAL_3_PENALTY=1.499588 PRIMER_LEFT_3_SEQUENCE=CTTTTTGGGGGAAAAATTTTTCCCC PRIMER_RIGHT_3_SEQUENCE=GGGGAAAAATTTTTCCCCCAAAAAG PRIMER_INTERNAL_3_SEQUENCE=CTTTTTGGGGGAAAAACCC PRIMER_LEFT_3=29,25 PRIMER_RIGHT_3=133,25 PRIMER_INTERNAL_3=89,19 PRIMER_LEFT_3_TM=67.774 PRIMER_RIGHT_3_TM=67.774 PRIMER_INTERNAL_3_TM=60.500 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=47.368 PRIMER_LEFT_3_SELF_ANY=18.00 PRIMER_RIGHT_3_SELF_ANY=18.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=18.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=25.00 PRIMER_PAIR_3_COMPL_END=25.00 PRIMER_PAIR_3_PRODUCT_SIZE=105 PRIMER_PAIR_4_PENALTY=15.547212 PRIMER_LEFT_4_PENALTY=7.773606 PRIMER_RIGHT_4_PENALTY=7.773606 PRIMER_INTERNAL_4_PENALTY=1.499588 PRIMER_LEFT_4_SEQUENCE=CCTTTTTGGGGGAAAAATTTTTCCC PRIMER_RIGHT_4_SEQUENCE=GAAAAATTTTTCCCCCAAAAAGGGG PRIMER_INTERNAL_4_SEQUENCE=CCCTTTTTGGGGGAAAAAC PRIMER_LEFT_4=28,25 PRIMER_RIGHT_4=130,25 PRIMER_INTERNAL_4=87,19 PRIMER_LEFT_4_TM=67.774 PRIMER_RIGHT_4_TM=67.774 PRIMER_INTERNAL_4_TM=60.500 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=47.368 PRIMER_LEFT_4_SELF_ANY=16.00 PRIMER_RIGHT_4_SELF_ANY=12.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=16.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=10.9000 PRIMER_PAIR_4_COMPL_ANY=23.00 PRIMER_PAIR_4_COMPL_END=19.00 PRIMER_PAIR_4_PRODUCT_SIZE=103 = SEQUENCE_ID=poly_x_3 SEQUENCE_TEMPLATE=AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTTGGGGGGGGGGGGGG PRIMER_MAX_POLY_X=6 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_LEFT_EXPLAIN=considered 155, long poly-x seq 57, ok 98 PRIMER_RIGHT_EXPLAIN=considered 155, long poly-x seq 72, ok 83 PRIMER_INTERNAL_EXPLAIN=considered 187, long poly-x seq 187, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_59 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=ok (excluded region is between G and N and end of left primer). PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_60 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=26,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (excluded region is between T and G at end of left primer). PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_61 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=26,-1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (negative excluded region size). PRIMER_ERROR=Negative EXCLUDED_REGION length = SEQUENCE_ID=boundary_test_62 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=1,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED REGION PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_63 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=2,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers PRIMER_LEFT_EXPLAIN=considered 1, overlap excluded region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_64 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=59,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with EXCLUDED_REGION outside of sequence PRIMER_ERROR=EXCLUDED_REGION beyond end of sequence = SEQUENCE_ID=boundary_test_65 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=58,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_66 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=56,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_67 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=56,2 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to pick primers and warn EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1 PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_68 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,0 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=ok (TARGET is between G and N and end of left primer). PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_69 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=26,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (TARGET is between T and G at end of left primer). PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 2, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_70 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=26,-1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (negative TARGET size). PRIMER_ERROR=Negative TARGET length = SEQUENCE_ID=boundary_test_71 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=1,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to find legal primers, and warn TARGET outside of INCLUDED REGION PRIMER_WARNING=TARGET outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_72 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=2,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers PRIMER_LEFT_EXPLAIN=considered 1, overlap target 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_73 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=59,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with TARGET outside of sequence PRIMER_ERROR=TARGET beyond end of sequence = SEQUENCE_ID=boundary_test_74 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=58,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to find primers (none spans target), and warn TARGET outside of INCLUDED_REGION PRIMER_WARNING=TARGET outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_75 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=56,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_76 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=56,2 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to pick primers, and warn TARGET outside of INCLUDED_REGION PRIMER_WARNING=TARGET outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1 PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, overlap target 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_77 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=27,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (target is at TTG^NNN) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_78 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=27,5 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (target is the middle run of N) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_79_1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=27,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_80 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=31,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_81 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=32,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_82 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=0,59 SEQUENCE_TARGET=32,0 0,1 58,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_83 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=0,59 SEQUENCE_TARGET=0,1 58,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (no target) PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_84 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=0,59 SEQUENCE_TARGET=0,1 32,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 8, too many Ns 7, ok 1 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_85 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=0,59 SEQUENCE_TARGET=32,0 58,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_86 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=35 PRIMER_MAX_SIZE=35 PRIMER_OPT_SIZE=35 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=75-75 SEQUENCE_TEMPLATE=NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (testing hard limit MAX_PRIMER_LENGTH) PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.202608 PRIMER_LEFT_0_PENALTY=2.838677 PRIMER_RIGHT_0_PENALTY=1.363932 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTATATATATATCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTATATATATATGAGT PRIMER_LEFT_0=2,35 PRIMER_RIGHT_0=76,35 PRIMER_LEFT_0_TM=62.839 PRIMER_RIGHT_0_TM=61.364 PRIMER_LEFT_0_GC_PERCENT=25.714 PRIMER_RIGHT_0_GC_PERCENT=25.714 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=13.00 PRIMER_PAIR_0_COMPL_END=8.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 = SEQUENCE_ID=boundary_test_71_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=1,1 27,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should warn TARGET outside of INCLUDED REGION PRIMER_WARNING=TARGET outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_72_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,56 PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one pair PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_73_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,56 PRIMER_PRODUCT_SIZE_RANGE=54-54 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (no primers with acceptable product size) PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 2, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_74_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,56 PRIMER_PRODUCT_SIZE_RANGE=54-55 55-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_75_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_76_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,56 PRIMER_PRODUCT_SIZE_RANGE=55-55 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_77_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,82 PRIMER_PRODUCT_SIZE_RANGE=55-55 82-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product second PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1 PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 2, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 PRIMER_PAIR_1_PENALTY=3.381274 PRIMER_LEFT_1_PENALTY=2.731673 PRIMER_RIGHT_1_PENALTY=0.649600 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_1=2,25 PRIMER_RIGHT_1=83,25 PRIMER_LEFT_1_TM=62.732 PRIMER_RIGHT_1_TM=60.650 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 = SEQUENCE_ID=boundary_test_78_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,82 PRIMER_PRODUCT_SIZE_RANGE=82-82 55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product first PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1 PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 2, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=83,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=3.381274 PRIMER_LEFT_1_PENALTY=2.731673 PRIMER_RIGHT_1_PENALTY=0.649600 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_1=2,25 PRIMER_RIGHT_1=56,25 PRIMER_LEFT_1_TM=62.732 PRIMER_RIGHT_1_TM=60.650 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_79_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,82 PRIMER_PRODUCT_SIZE_RANGE=55-55 55-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product second; PRIMER_PAIR_EXPLAIN will be incorrect (too many pairs ok) because the PRIMER_PRODUCT_SIZE_RANGE's overlap PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1 PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2 PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 1, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 PRIMER_PAIR_1_PENALTY=3.381274 PRIMER_LEFT_1_PENALTY=2.731673 PRIMER_RIGHT_1_PENALTY=0.649600 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_1=2,25 PRIMER_RIGHT_1=83,25 PRIMER_LEFT_1_TM=62.732 PRIMER_RIGHT_1_TM=60.650 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 = SEQUENCE_ID=boundary_test_80_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,82 PRIMER_PRODUCT_SIZE_RANGE=82-82 55-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product first PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1 PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2 PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 1, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=83,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=3.381274 PRIMER_LEFT_1_PENALTY=2.731673 PRIMER_RIGHT_1_PENALTY=0.649600 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_1=2,25 PRIMER_RIGHT_1=56,25 PRIMER_LEFT_1_TM=62.732 PRIMER_RIGHT_1_TM=60.650 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_81_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing pairwise complementarity checking) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.137556 PRIMER_LEFT_0_PENALTY=2.963671 PRIMER_RIGHT_0_PENALTY=1.173886 PRIMER_LEFT_0_SEQUENCE=AATGTGATTGCATCAAATGTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTGGAAATGACAATCACATT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.964 PRIMER_RIGHT_0_TM=61.174 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=11.00 PRIMER_PAIR_0_COMPL_END=10.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_82_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=10.99 PRIMER_PAIR_MAX_COMPL_ANY=10.99 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (self any for pairs) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, high any compl 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_83_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing pairwise complementarity checking) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.137556 PRIMER_LEFT_0_PENALTY=2.963671 PRIMER_RIGHT_0_PENALTY=1.173886 PRIMER_LEFT_0_SEQUENCE=AATGTGATTGCATCAAATGTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTGGAAATGACAATCACATT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.964 PRIMER_RIGHT_0_TM=61.174 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=11.00 PRIMER_PAIR_0_COMPL_END=10.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_84_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=9.99 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=9.99 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (self end for pairs) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, high end compl 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_85_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of product size ranges) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.137556 PRIMER_LEFT_0_PENALTY=2.963671 PRIMER_RIGHT_0_PENALTY=1.173886 PRIMER_LEFT_0_SEQUENCE=AATGTGATTGCATCAAATGTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTGGAAATGACAATCACATT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.964 PRIMER_RIGHT_0_TM=61.174 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=11.00 PRIMER_PAIR_0_COMPL_END=10.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_86 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TARGET=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of targets) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.137556 PRIMER_LEFT_0_PENALTY=2.963671 PRIMER_RIGHT_0_PENALTY=1.173886 PRIMER_LEFT_0_SEQUENCE=AATGTGATTGCATCAAATGTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTGGAAATGACAATCACATT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.964 PRIMER_RIGHT_0_TM=61.174 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=11.00 PRIMER_PAIR_0_COMPL_END=10.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_87 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TARGET=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (testing number of targets) PRIMER_ERROR=Too many elements for tag SEQUENCE_TARGET = SEQUENCE_ID=boundary_test_88 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_EXCLUDED_REGION=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of excluded regions) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.137556 PRIMER_LEFT_0_PENALTY=2.963671 PRIMER_RIGHT_0_PENALTY=1.173886 PRIMER_LEFT_0_SEQUENCE=AATGTGATTGCATCAAATGTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTGGAAATGACAATCACATT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.964 PRIMER_RIGHT_0_TM=61.174 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=11.00 PRIMER_PAIR_0_COMPL_END=10.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_89 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_EXCLUDED_REGION=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (testing number of excluded regions) PRIMER_ERROR=Too many elements for tag SEQUENCE_EXCLUDED_REGION = SEQUENCE_ID=boundary_test_90 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MAX_END_STABILITY=6.9 P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_91 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MAX_END_STABILITY=6.8999 P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, high 3' stability 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_92 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,1 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=100 SEQUENCE_TEMPLATE=ATCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCGG PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 2, ok 3 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3 PRIMER_PAIR_EXPLAIN=considered 9, unacceptable product size 8, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=403.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=400.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=56,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=400.000000 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_93 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,1 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=100 SEQUENCE_TEMPLATE=ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=402.849012 PRIMER_LEFT_0_PENALTY=200.942558 PRIMER_RIGHT_0_PENALTY=201.906454 PRIMER_LEFT_0_SEQUENCE=ATCACGTCATCTCATCAAATTTGAT PRIMER_RIGHT_0_SEQUENCE=ATTGTTTGGAAATGACAGTGAGTAT PRIMER_LEFT_0=0,25 PRIMER_RIGHT_0=54,25 PRIMER_LEFT_0_TM=60.943 PRIMER_RIGHT_0_TM=58.094 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=12.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_POSITION_PENALTY=200.000000 PRIMER_RIGHT_0_POSITION_PENALTY=200.000000 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=5.3000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_94 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=100 SEQUENCE_TEMPLATE=ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=2.849012 PRIMER_LEFT_0_PENALTY=0.942558 PRIMER_RIGHT_0_PENALTY=1.906454 PRIMER_LEFT_0_SEQUENCE=ATCACGTCATCTCATCAAATTTGAT PRIMER_RIGHT_0_SEQUENCE=ATTGTTTGGAAATGACAGTGAGTAT PRIMER_LEFT_0=0,25 PRIMER_RIGHT_0=54,25 PRIMER_LEFT_0_TM=60.943 PRIMER_RIGHT_0_TM=58.094 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=12.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=5.3000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_95 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,10 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=0 PRIMER_INSIDE_PENALTY=1000 SEQUENCE_TEMPLATE=ATCACGTCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATNCGG PRIMER_LEFT_EXPLAIN=considered 5, ok 5 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=7002.383469 PRIMER_LEFT_0_PENALTY=0.942558 PRIMER_RIGHT_0_PENALTY=7001.440912 PRIMER_LEFT_0_SEQUENCE=ATCACGTCATCTCATCAAATTTGAT PRIMER_RIGHT_0_SEQUENCE=ATTGTTTGGAAATGACAGTGAGTAC PRIMER_LEFT_0=0,25 PRIMER_RIGHT_0=54,25 PRIMER_LEFT_0_TM=60.943 PRIMER_RIGHT_0_TM=58.559 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=12.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=7000.000000 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_96 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,10 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=0 PRIMER_INSIDE_PENALTY=1000 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=5004.910264 PRIMER_LEFT_0_PENALTY=2000.754679 PRIMER_RIGHT_0_PENALTY=3004.155585 PRIMER_LEFT_0_SEQUENCE=CATCATCTCATCAAATTTGATTGAT PRIMER_RIGHT_0_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA PRIMER_LEFT_0=4,25 PRIMER_RIGHT_0=58,25 PRIMER_LEFT_0_TM=59.245 PRIMER_RIGHT_0_TM=64.156 PRIMER_LEFT_0_GC_PERCENT=28.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=12.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_POSITION_PENALTY=2000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=3000.000000 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_97 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=0 PRIMER_INSIDE_PENALTY=1000 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.910264 PRIMER_LEFT_0_PENALTY=0.754679 PRIMER_RIGHT_0_PENALTY=4.155585 PRIMER_LEFT_0_SEQUENCE=CATCATCTCATCAAATTTGATTGAT PRIMER_RIGHT_0_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA PRIMER_LEFT_0=4,25 PRIMER_RIGHT_0=58,25 PRIMER_LEFT_0_TM=59.245 PRIMER_RIGHT_0_TM=64.156 PRIMER_LEFT_0_GC_PERCENT=28.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=12.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=test_098 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATTTTACTCACTGTCATTTCCAAACAATACGG PRIMER_INSIDE_PENALTY=-1.0 PRIMER_PRODUCT_MAX_TM=62.4120 P3_COMMENT=Should succeed. PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.910264 PRIMER_LEFT_0_PENALTY=0.754679 PRIMER_RIGHT_0_PENALTY=4.155585 PRIMER_LEFT_0_SEQUENCE=CATCATCTCATCAAATTTGATTGAT PRIMER_RIGHT_0_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA PRIMER_LEFT_0=4,25 PRIMER_RIGHT_0=58,25 PRIMER_LEFT_0_TM=59.245 PRIMER_RIGHT_0_TM=64.156 PRIMER_LEFT_0_GC_PERCENT=28.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=12.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 PRIMER_PAIR_0_PRODUCT_TM=62.4120 PRIMER_PAIR_0_PRODUCT_TM_OLIGO_TM_DIFF=3.1667 PRIMER_PAIR_0_T_OPT_A=46.5620 = SEQUENCE_ID=test_099 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_PRODUCT_MAX_TM=62.4119 P3_COMMENT=Should fail. PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, high product Tm 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=test_100 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_PRODUCT_MAX_TM=1000000 P3_COMMENT=Make sure that product tm output gets turned off again. PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.910264 PRIMER_LEFT_0_PENALTY=0.754679 PRIMER_RIGHT_0_PENALTY=4.155585 PRIMER_LEFT_0_SEQUENCE=CATCATCTCATCAAATTTGATTGAT PRIMER_RIGHT_0_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA PRIMER_LEFT_0=4,25 PRIMER_RIGHT_0=58,25 PRIMER_LEFT_0_TM=59.245 PRIMER_RIGHT_0_TM=64.156 PRIMER_LEFT_0_GC_PERCENT=28.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=12.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=test_101 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_PRODUCT_MIN_TM=62.4119 P3_COMMENT=Should succeed. PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.910264 PRIMER_LEFT_0_PENALTY=0.754679 PRIMER_RIGHT_0_PENALTY=4.155585 PRIMER_LEFT_0_SEQUENCE=CATCATCTCATCAAATTTGATTGAT PRIMER_RIGHT_0_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA PRIMER_LEFT_0=4,25 PRIMER_RIGHT_0=58,25 PRIMER_LEFT_0_TM=59.245 PRIMER_RIGHT_0_TM=64.156 PRIMER_LEFT_0_GC_PERCENT=28.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=12.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 PRIMER_PAIR_0_PRODUCT_TM=63.9029 PRIMER_PAIR_0_PRODUCT_TM_OLIGO_TM_DIFF=4.6576 PRIMER_PAIR_0_T_OPT_A=47.6056 = SEQUENCE_ID=test_102 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_PRODUCT_MIN_TM=63.9030 P3_COMMENT=Should fail. PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, low product Tm 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=test_103 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_PRODUCT_MIN_TM=-1000000 P3_COMMENT=Make sure that product tm output gets turned off again. PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.910264 PRIMER_LEFT_0_PENALTY=0.754679 PRIMER_RIGHT_0_PENALTY=4.155585 PRIMER_LEFT_0_SEQUENCE=CATCATCTCATCAAATTTGATTGAT PRIMER_RIGHT_0_SEQUENCE=CCGTATTGTTTGGAAATGACAGTGA PRIMER_LEFT_0=4,25 PRIMER_RIGHT_0=58,25 PRIMER_LEFT_0_TM=59.245 PRIMER_RIGHT_0_TM=64.156 PRIMER_LEFT_0_GC_PERCENT=28.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=12.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = primer3-2.3.6/test/primer_obj_fn_input0000664000077300007730000002061412231156245017015 0ustar ioanaioanaSEQUENCE_ID=io_compl PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 PRIMER_PAIR_WT_IO_PENALTY=1 PRIMER_PAIR_WT_PR_PENALTY=0 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=1 PRIMER_INTERNAL_WT_SELF_END=2 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG = SEQUENCE_ID=diff_tm PRIMER_PAIR_WT_IO_PENALTY=0 PRIMER_PAIR_WT_PR_PENALTY=1 PRIMER_PAIR_WT_DIFF_TM=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_TASK=pick_pcr_primers SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG = SEQUENCE_ID=num_NS PRIMER_PAIR_WT_DIFF_TM=0 PRIMER_WT_NUM_NS=1 PRIMER_MAX_NS_ACCEPTED=15 SEQUENCE_TEMPLATE=AAGCCGNGGATGANTTCACTTTCNGACCNTGCAGAGCCGANTTTCACNCACGAGNGAAGACTNCGCCAGCCTTGCCCCTNGGGCCTCTNCCTCAACTCNCCATGATGNCCGTCAGGNNNNNNNNNCAGGCGNACTGAGGCGTGGTNCGCGGGGCTCAGGNNNNNNNNNCCTGCGCCGG = SEQUENCE_ID=product_size PRIMER_WT_NUM_NS=0 PRIMER_PRODUCT_OPT_SIZE=60 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=1 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PAIR_WT_DIFF_TM=0 PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_WT_LIBRARY_MISPRIMING=0 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 PRIMER_PAIR_MAX_DIFF_TM=3 PRIMER_MISPRIMING_LIBRARY= = SEQUENCE_ID=rep_sim PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0 PRIMER_MAX_LIBRARY_MISPRIMING=20 PRIMER_WT_LIBRARY_MISPRIMING=1 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC = SEQUENCE_ID=pair_rep_sim PRIMER_WT_LIBRARY_MISPRIMING=0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC = SEQUENCE_ID=product_tm PRIMER_PAIR_WT_PRODUCT_TM_LT=1 PRIMER_PAIR_WT_PRODUCT_TM_GT=1 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0 PRIMER_PRODUCT_OPT_TM=80 PRIMER_MISPRIMING_LIBRARY= SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCT = SEQUENCE_ID=primer_compl PRIMER_MIN_GC=1.0 PRIMER_MAX_GC=99.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0 PRIMER_PRODUCT_OPT_SIZE=200 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0 PRIMER_PRODUCT_OPT_TM=0 PRIMER_WT_LIBRARY_MISPRIMING=0 PRIMER_WT_SELF_ANY=1 PRIMER_WT_SELF_END=2 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAATAGACATCATAATTAGATACAC = PRIMER_MISPRIMING_LIBRARY= PRIMER_INTERNAL_MISHYB_LIBRARY=not_for_production.lib PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_WT_SELF_ANY=0 PRIMER_WT_SELF_END=0 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=1 PRIMER_PAIR_WT_COMPL_ANY=0 PRIMER_PAIR_WT_COMPL_END=0 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=20 PRIMER_PAIR_WT_PR_PENALTY=0 PRIMER_PAIR_WT_IO_PENALTY=1 SEQUENCE_ID=io_rep_sim SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATAACCTATACATAATGTGAAATATAATTTTT = SEQUENCE_ID=end_stability PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_INTERNAL_MISHYB_LIBRARY= PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PAIR_WT_PR_PENALTY=1 PRIMER_PAIR_WT_IO_PENALTY=0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0 PRIMER_WT_END_STABILITY=1 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCG PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 = SEQUENCE_ID=position_penalty SEQUENCE_TARGET=298,45 PRIMER_OUTSIDE_PENALTY=20.0 PRIMER_INSIDE_PENALTY=1000.0 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=36-2000 PRIMER_WT_POS_PENALTY=1 PRIMER_WT_END_STABILITY=0 SEQUENCE_TEMPLATE=TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTCCCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACTTTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCTCTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCAGGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTCCCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAA = SEQUENCE_ID=sequence_quality PRIMER_WT_POS_PENALTY=0 PRIMER_WT_SEQ_QUAL=1 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_QUALITY_RANGE_MAX=70 SEQUENCE_QUALITY=10 20 30 40 50 50 50 50 50 50 50 60 50 50 60 50 50 50 50 50 50 55 50 50 55 50 60 50 50 50 50 60 60 60 60 60 60 60 20 20 20 30 30 30 50 50 30 53 30 50 60 60 60 60 60 60 60 50 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 20 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 = SEQUENCE_ID=sequence_quality_io PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_SEQ_QUAL=1 PRIMER_PAIR_MAX_DIFF_TM=20 PRIMER_MIN_TM=20 PRIMER_MAX_TM=100 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 PRIMER_INTERNAL_MAX_SELF_ANY=20 PRIMER_INTERNAL_MAX_SELF_END=20 PRIMER_INTERNAL_MIN_TM=20 PRIMER_INTERNAL_MAX_TM=100 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=20 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=20 PRIMER_PAIR_MAX_COMPL_ANY=20 PRIMER_PAIR_MAX_COMPL_END=20 PRIMER_INTERNAL_WT_END_QUAL=2 PRIMER_PAIR_WT_PR_PENALTY=0 PRIMER_PAIR_WT_IO_PENALTY=1 PRIMER_WT_SEQ_QUAL=0 PRIMER_WT_END_QUAL=0 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATC SEQUENCE_QUALITY=10 20 30 40 50 50 50 50 50 50 50 60 50 50 60 50 50 50 50 50 50 55 50 50 55 50 60 50 50 50 50 60 60 60 60 60 60 60 20 20 20 30 30 30 50 50 30 53 30 50 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 70 70 70 70 70 70 70 70 70 70 70 70 70 70 = SEQUENCE_ID=io_gc_content PRIMER_WT_SEQ_QUAL=0 PRIMER_INTERNAL_WT_SEQ_QUAL=0 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_GC=40 PRIMER_INTERNAL_OPT_GC_PERCENT=50 PRIMER_INTERNAL_MAX_GC=60 PRIMER_INTERNAL_WT_GC_PERCENT_GT=1 PRIMER_INTERNAL_WT_GC_PERCENT_LT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PAIR_WT_IO_PENALTY=1 PRIMER_PAIR_WT_PR_PENALTY=0 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG = SEQUENCE_ID=primer_gc_content PRIMER_WT_SEQ_QUAL=0 PRIMER_INTERNAL_WT_SEQ_QUAL=0 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 PRIMER_MIN_GC=40 PRIMER_OPT_GC_PERCENT=50 PRIMER_MAX_GC=60 PRIMER_WT_GC_PERCENT_GT=1 PRIMER_WT_GC_PERCENT_LT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PAIR_WT_PR_PENALTY=1 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG = primer3-2.3.6/test/primer_internal_output0000664000077300007730000014762412231156245017610 0ustar ioanaioanaSEQUENCE_ID=boundary_test_00 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_INTERNAL_MIN_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_INTERNAL_MAX_SELF_ANY=100 PRIMER_INTERNAL_MAX_SELF_END=100 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_INTERNAL_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, no internal oligo 2, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-100 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 30, too many Ns 28, ok 2 PRIMER_RIGHT_EXPLAIN=considered 30, too many Ns 29, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=2.731673 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=29,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=62.732 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=10.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_02 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=2.731673 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=29,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=62.732 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=10.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_05 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_MIN_SIZE=24 PRIMER_MAX_SIZE=24 PRIMER_OPT_SIZE=24 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.330996 PRIMER_LEFT_0_PENALTY=0.243965 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=2.731673 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_LEFT_0=2,24 PRIMER_RIGHT_0=81,24 PRIMER_INTERNAL_0=29,25 PRIMER_LEFT_0_TM=60.244 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=62.732 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=10.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 1, ok 4 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 59, ok 1 PRIMER_PAIR_EXPLAIN=considered 8, unacceptable product size 6, no internal oligo 2, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_11_2 PRIMER_MAX_NS_ACCEPTED=2 PRIMER_INTERNAL_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 52, ok 8 PRIMER_PAIR_EXPLAIN=considered 10, unacceptable product size 8, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=2 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=0.893850 PRIMER_LEFT_0_PENALTY=0.806819 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=3.221174 PRIMER_LEFT_0_SEQUENCE=CACGTCATCNNATCAAATTTGATT PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCNNATCAAATTTGATTG PRIMER_LEFT_0=2,24 PRIMER_RIGHT_0=81,24 PRIMER_INTERNAL_0=29,25 PRIMER_LEFT_0_TM=60.807 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=63.221 PRIMER_LEFT_0_GC_PERCENT=31.818 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=11.50 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=11.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=2.546959 PRIMER_LEFT_1_PENALTY=0.806819 PRIMER_RIGHT_1_PENALTY=1.740140 PRIMER_INTERNAL_1_PENALTY=3.221174 PRIMER_LEFT_1_SEQUENCE=ACGTCATCNNATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=NGAATTGTTTGGAAATGACAGTGA PRIMER_INTERNAL_1_SEQUENCE=CACGTCATCNNATCAAATTTGATTG PRIMER_LEFT_1=3,24 PRIMER_RIGHT_1=82,24 PRIMER_INTERNAL_1=29,25 PRIMER_LEFT_1_TM=60.807 PRIMER_RIGHT_1_TM=61.740 PRIMER_INTERNAL_1_TM=63.221 PRIMER_LEFT_1_GC_PERCENT=31.818 PRIMER_RIGHT_1_GC_PERCENT=34.783 PRIMER_INTERNAL_1_GC_PERCENT=34.783 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=12.00 PRIMER_LEFT_1_SELF_END=11.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=11.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_12 PRIMER_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 52, ok 8 PRIMER_PAIR_EXPLAIN=considered 10, unacceptable product size 8, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=2 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=0.893850 PRIMER_LEFT_0_PENALTY=0.806819 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=3.221174 PRIMER_LEFT_0_SEQUENCE=CACGTCATCNNATCAAATTTGATT PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCNNATCAAATTTGATTG PRIMER_LEFT_0=2,24 PRIMER_RIGHT_0=81,24 PRIMER_INTERNAL_0=29,25 PRIMER_LEFT_0_TM=60.807 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=63.221 PRIMER_LEFT_0_GC_PERCENT=31.818 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=11.50 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=11.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=2.546959 PRIMER_LEFT_1_PENALTY=0.806819 PRIMER_RIGHT_1_PENALTY=1.740140 PRIMER_INTERNAL_1_PENALTY=3.221174 PRIMER_LEFT_1_SEQUENCE=ACGTCATCNNATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=NGAATTGTTTGGAAATGACAGTGA PRIMER_INTERNAL_1_SEQUENCE=CACGTCATCNNATCAAATTTGATTG PRIMER_LEFT_1=3,24 PRIMER_RIGHT_1=82,24 PRIMER_INTERNAL_1=29,25 PRIMER_LEFT_1_TM=60.807 PRIMER_RIGHT_1_TM=61.740 PRIMER_INTERNAL_1_TM=63.221 PRIMER_LEFT_1_GC_PERCENT=31.818 PRIMER_RIGHT_1_GC_PERCENT=34.783 PRIMER_INTERNAL_1_GC_PERCENT=34.783 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=12.00 PRIMER_LEFT_1_SELF_END=11.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=11.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_13 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 23, ok 37 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=5.394230 PRIMER_LEFT_0_PENALTY=5.307199 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=3.800256 PRIMER_LEFT_0_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAA PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTNN PRIMER_LEFT_0=2,24 PRIMER_RIGHT_0=81,24 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=54.693 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=56.200 PRIMER_LEFT_0_GC_PERCENT=0.000 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=0.000 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_14 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=.0001 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 2, ok 3 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 25, ok 35 PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.843553 PRIMER_LEFT_0_PENALTY=4.756523 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=3.800256 PRIMER_LEFT_0_SEQUENCE=AAAAAAAAAAAAAAAAACAAAAAA PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTNN PRIMER_LEFT_0=2,24 PRIMER_RIGHT_0=81,24 PRIMER_INTERNAL_0=29,25 PRIMER_LEFT_0_TM=55.243 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=56.200 PRIMER_LEFT_0_GC_PERCENT=4.167 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=0.000 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_15 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=20 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 25, ok 35 PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=29.395694 PRIMER_LEFT_0_PENALTY=29.308664 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=4.161950 PRIMER_LEFT_0_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTTT PRIMER_LEFT_0=2,24 PRIMER_RIGHT_0=81,24 PRIMER_INTERNAL_0=32,25 PRIMER_LEFT_0_TM=89.309 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=55.838 PRIMER_LEFT_0_GC_PERCENT=100.000 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=0.000 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=1.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_16 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_PRODUCT_SIZE_RANGE=50-200 PRIMER_INTERNAL_MAX_NS_ACCEPTED=5 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 8, ok 2 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 8, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 35, ok 35 PRIMER_PAIR_EXPLAIN=considered 4, no internal oligo 4, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_17 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=20 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 31, ok 4 PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 31, ok 4 PRIMER_INTERNAL_EXPLAIN=considered 60, GC content failed 29, ok 31 PRIMER_PAIR_EXPLAIN=considered 16, unacceptable product size 4, no internal oligo 12, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_18 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INTERNAL_EXCLUDED_REGION=10,50 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 62, too many Ns 53, ok 9 PRIMER_RIGHT_EXPLAIN=considered 62, too many Ns 53, ok 9 PRIMER_INTERNAL_EXPLAIN=considered 87, too many Ns 39, overlap excluded region 40, ok 8 PRIMER_PAIR_EXPLAIN=considered 81, unacceptable product size 49, no internal oligo 32, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_19 PRIMER_NUM_RETURN=7 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 SEQUENCE_INTERNAL_EXCLUDED_REGION=10,50 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNNCCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 66, too many Ns 57, ok 9 PRIMER_RIGHT_EXPLAIN=considered 66, too many Ns 57, ok 9 PRIMER_INTERNAL_EXPLAIN=considered 91, too many Ns 84, overlap excluded region 5, ok 2 PRIMER_PAIR_EXPLAIN=considered 20, unacceptable product size 3, no internal oligo 10, ok 7 PRIMER_LEFT_NUM_RETURNED=7 PRIMER_RIGHT_NUM_RETURNED=7 PRIMER_INTERNAL_NUM_RETURNED=7 PRIMER_PAIR_NUM_RETURNED=7 PRIMER_PAIR_0_PENALTY=0.330996 PRIMER_LEFT_0_PENALTY=0.243965 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=5.578209 PRIMER_LEFT_0_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_0=3,24 PRIMER_RIGHT_0=112,24 PRIMER_INTERNAL_0=60,25 PRIMER_LEFT_0_TM=60.244 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=65.578 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=52.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=110 PRIMER_PAIR_1_PENALTY=0.330996 PRIMER_LEFT_1_PENALTY=0.243965 PRIMER_RIGHT_1_PENALTY=0.087031 PRIMER_INTERNAL_1_PENALTY=5.578209 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_1_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_1=2,24 PRIMER_RIGHT_1=112,24 PRIMER_INTERNAL_1=60,25 PRIMER_LEFT_1_TM=60.244 PRIMER_RIGHT_1_TM=59.913 PRIMER_INTERNAL_1_TM=65.578 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=37.500 PRIMER_INTERNAL_1_GC_PERCENT=52.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=111 PRIMER_PAIR_2_PENALTY=1.261475 PRIMER_LEFT_2_PENALTY=0.243965 PRIMER_RIGHT_2_PENALTY=1.017510 PRIMER_INTERNAL_2_PENALTY=5.578209 PRIMER_LEFT_2_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_2_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_2_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_2=3,24 PRIMER_RIGHT_2=111,24 PRIMER_INTERNAL_2=60,25 PRIMER_LEFT_2_TM=60.244 PRIMER_RIGHT_2_TM=58.982 PRIMER_INTERNAL_2_TM=65.578 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=33.333 PRIMER_INTERNAL_2_GC_PERCENT=52.000 PRIMER_LEFT_2_SELF_ANY=12.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=10.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=109 PRIMER_PAIR_3_PENALTY=1.261475 PRIMER_LEFT_3_PENALTY=0.243965 PRIMER_RIGHT_3_PENALTY=1.017510 PRIMER_INTERNAL_3_PENALTY=5.578209 PRIMER_LEFT_3_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_3_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_3=2,24 PRIMER_RIGHT_3=111,24 PRIMER_INTERNAL_3=60,25 PRIMER_LEFT_3_TM=60.244 PRIMER_RIGHT_3_TM=58.982 PRIMER_INTERNAL_3_TM=65.578 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=52.000 PRIMER_LEFT_3_SELF_ANY=12.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=10.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=110 PRIMER_PAIR_4_PENALTY=8.005859 PRIMER_LEFT_4_PENALTY=7.918829 PRIMER_RIGHT_4_PENALTY=0.087031 PRIMER_INTERNAL_4_PENALTY=5.578209 PRIMER_LEFT_4_SEQUENCE=AAGCTAAGCTTGCCCGTATCCCCT PRIMER_RIGHT_4_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_4_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_4=29,24 PRIMER_RIGHT_4=112,24 PRIMER_INTERNAL_4=60,25 PRIMER_LEFT_4_TM=67.919 PRIMER_RIGHT_4_TM=59.913 PRIMER_INTERNAL_4_TM=65.578 PRIMER_LEFT_4_GC_PERCENT=54.167 PRIMER_RIGHT_4_GC_PERCENT=37.500 PRIMER_INTERNAL_4_GC_PERCENT=52.000 PRIMER_LEFT_4_SELF_ANY=9.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=10.9000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=84 PRIMER_PAIR_5_PENALTY=8.936339 PRIMER_LEFT_5_PENALTY=7.918829 PRIMER_RIGHT_5_PENALTY=1.017510 PRIMER_INTERNAL_5_PENALTY=5.578209 PRIMER_LEFT_5_SEQUENCE=AAGCTAAGCTTGCCCGTATCCCCT PRIMER_RIGHT_5_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_5_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_5=29,24 PRIMER_RIGHT_5=111,24 PRIMER_INTERNAL_5=60,25 PRIMER_LEFT_5_TM=67.919 PRIMER_RIGHT_5_TM=58.982 PRIMER_INTERNAL_5_TM=65.578 PRIMER_LEFT_5_GC_PERCENT=54.167 PRIMER_RIGHT_5_GC_PERCENT=33.333 PRIMER_INTERNAL_5_GC_PERCENT=52.000 PRIMER_LEFT_5_SELF_ANY=9.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_INTERNAL_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_INTERNAL_5_SELF_END=1.00 PRIMER_LEFT_5_END_STABILITY=10.9000 PRIMER_RIGHT_5_END_STABILITY=6.4000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=83 PRIMER_PAIR_6_PENALTY=9.315217 PRIMER_LEFT_6_PENALTY=9.228187 PRIMER_RIGHT_6_PENALTY=0.087031 PRIMER_INTERNAL_6_PENALTY=5.578209 PRIMER_LEFT_6_SEQUENCE=AGCTAAGCTTGCCCGTATCCCCTG PRIMER_RIGHT_6_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_6_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_6=30,24 PRIMER_RIGHT_6=112,24 PRIMER_INTERNAL_6=60,25 PRIMER_LEFT_6_TM=69.228 PRIMER_RIGHT_6_TM=59.913 PRIMER_INTERNAL_6_TM=65.578 PRIMER_LEFT_6_GC_PERCENT=58.333 PRIMER_RIGHT_6_GC_PERCENT=37.500 PRIMER_INTERNAL_6_GC_PERCENT=52.000 PRIMER_LEFT_6_SELF_ANY=7.00 PRIMER_RIGHT_6_SELF_ANY=4.00 PRIMER_INTERNAL_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=1.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_INTERNAL_6_SELF_END=1.00 PRIMER_LEFT_6_END_STABILITY=9.7000 PRIMER_RIGHT_6_END_STABILITY=6.4000 PRIMER_PAIR_6_COMPL_ANY=3.00 PRIMER_PAIR_6_COMPL_END=2.00 PRIMER_PAIR_6_PRODUCT_SIZE=83 = SEQUENCE_ID=boundary_test_20 PRIMER_NUM_RETURN=5 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=100-300 SEQUENCE_INTERNAL_EXCLUDED_REGION=5,50 SEQUENCE_INTERNAL_EXCLUDED_REGION=60,15 SEQUENCE_INCLUDED_REGION=2,250 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATACGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT PRIMER_LEFT_EXPLAIN=considered 151, too many Ns 99, ok 52 PRIMER_RIGHT_EXPLAIN=considered 151, too many Ns 148, ok 3 PRIMER_INTERNAL_EXPLAIN=considered 226, too many Ns 175, overlap excluded region 47, ok 4 PRIMER_PAIR_EXPLAIN=considered 14, no internal oligo 8, ok 6 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.330996 PRIMER_LEFT_0_PENALTY=0.243965 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=3.811648 PRIMER_LEFT_0_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG PRIMER_LEFT_0=3,24 PRIMER_RIGHT_0=251,24 PRIMER_INTERNAL_0=75,25 PRIMER_LEFT_0_TM=60.244 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=63.812 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=52.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=12.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=249 PRIMER_PAIR_1_PENALTY=0.330996 PRIMER_LEFT_1_PENALTY=0.243965 PRIMER_RIGHT_1_PENALTY=0.087031 PRIMER_INTERNAL_1_PENALTY=3.811648 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_1_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG PRIMER_LEFT_1=2,24 PRIMER_RIGHT_1=251,24 PRIMER_INTERNAL_1=75,25 PRIMER_LEFT_1_TM=60.244 PRIMER_RIGHT_1_TM=59.913 PRIMER_INTERNAL_1_TM=63.812 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=37.500 PRIMER_INTERNAL_1_GC_PERCENT=52.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=12.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=12.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=250 PRIMER_PAIR_2_PENALTY=0.386435 PRIMER_LEFT_2_PENALTY=0.299404 PRIMER_RIGHT_2_PENALTY=0.087031 PRIMER_INTERNAL_2_PENALTY=3.811648 PRIMER_LEFT_2_SEQUENCE=GGAGACGATACGGTAAGCTAGCTA PRIMER_RIGHT_2_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_2_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG PRIMER_LEFT_2=50,24 PRIMER_RIGHT_2=251,24 PRIMER_INTERNAL_2=75,25 PRIMER_LEFT_2_TM=60.299 PRIMER_RIGHT_2_TM=59.913 PRIMER_INTERNAL_2_TM=63.812 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=37.500 PRIMER_INTERNAL_2_GC_PERCENT=52.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=12.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=12.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=202 PRIMER_PAIR_3_PENALTY=1.261475 PRIMER_LEFT_3_PENALTY=0.243965 PRIMER_RIGHT_3_PENALTY=1.017510 PRIMER_INTERNAL_3_PENALTY=3.811648 PRIMER_LEFT_3_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_3_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG PRIMER_LEFT_3=3,24 PRIMER_RIGHT_3=250,24 PRIMER_INTERNAL_3=75,25 PRIMER_LEFT_3_TM=60.244 PRIMER_RIGHT_3_TM=58.982 PRIMER_INTERNAL_3_TM=63.812 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=52.000 PRIMER_LEFT_3_SELF_ANY=12.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=12.00 PRIMER_LEFT_3_SELF_END=10.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=12.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=248 PRIMER_PAIR_4_PENALTY=1.261475 PRIMER_LEFT_4_PENALTY=0.243965 PRIMER_RIGHT_4_PENALTY=1.017510 PRIMER_INTERNAL_4_PENALTY=3.811648 PRIMER_LEFT_4_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_4_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_4_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG PRIMER_LEFT_4=2,24 PRIMER_RIGHT_4=250,24 PRIMER_INTERNAL_4=75,25 PRIMER_LEFT_4_TM=60.244 PRIMER_RIGHT_4_TM=58.982 PRIMER_INTERNAL_4_TM=63.812 PRIMER_LEFT_4_GC_PERCENT=33.333 PRIMER_RIGHT_4_GC_PERCENT=33.333 PRIMER_INTERNAL_4_GC_PERCENT=52.000 PRIMER_LEFT_4_SELF_ANY=12.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=12.00 PRIMER_LEFT_4_SELF_END=10.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=12.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=249 = SEQUENCE_ID=boundary_test_21 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_INTERNAL_MIN_TM=55.7 PRIMER_INTERNAL_MAX_TM=55.9 PRIMER_INTERNAL_OPT_TM=55.8 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 77, too many Ns 27, ok 50 PRIMER_RIGHT_EXPLAIN=considered 77, too many Ns 28, ok 49 PRIMER_INTERNAL_EXPLAIN=considered 152, too many Ns 57, low tm 1, high tm 93, ok 1 PRIMER_PAIR_EXPLAIN=considered 323, unacceptable product size 223, no internal oligo 87, ok 13 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.330996 PRIMER_LEFT_0_PENALTY=0.243965 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=0.038050 PRIMER_LEFT_0_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_LEFT_0=3,24 PRIMER_RIGHT_0=173,24 PRIMER_INTERNAL_0=61,25 PRIMER_LEFT_0_TM=60.244 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=55.838 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=0.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=171 PRIMER_PAIR_1_PENALTY=0.330996 PRIMER_LEFT_1_PENALTY=0.243965 PRIMER_RIGHT_1_PENALTY=0.087031 PRIMER_INTERNAL_1_PENALTY=0.038050 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_1_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_LEFT_1=2,24 PRIMER_RIGHT_1=173,24 PRIMER_INTERNAL_1=61,25 PRIMER_LEFT_1_TM=60.244 PRIMER_RIGHT_1_TM=59.913 PRIMER_INTERNAL_1_TM=55.838 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=37.500 PRIMER_INTERNAL_1_GC_PERCENT=0.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=0.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=172 PRIMER_PAIR_2_PENALTY=1.261475 PRIMER_LEFT_2_PENALTY=0.243965 PRIMER_RIGHT_2_PENALTY=1.017510 PRIMER_INTERNAL_2_PENALTY=0.038050 PRIMER_LEFT_2_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_2_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_2_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_LEFT_2=3,24 PRIMER_RIGHT_2=172,24 PRIMER_INTERNAL_2=61,25 PRIMER_LEFT_2_TM=60.244 PRIMER_RIGHT_2_TM=58.982 PRIMER_INTERNAL_2_TM=55.838 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=33.333 PRIMER_INTERNAL_2_GC_PERCENT=0.000 PRIMER_LEFT_2_SELF_ANY=12.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=0.00 PRIMER_LEFT_2_SELF_END=10.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=170 PRIMER_PAIR_3_PENALTY=1.261475 PRIMER_LEFT_3_PENALTY=0.243965 PRIMER_RIGHT_3_PENALTY=1.017510 PRIMER_INTERNAL_3_PENALTY=0.038050 PRIMER_LEFT_3_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_3_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_LEFT_3=2,24 PRIMER_RIGHT_3=172,24 PRIMER_INTERNAL_3=61,25 PRIMER_LEFT_3_TM=60.244 PRIMER_RIGHT_3_TM=58.982 PRIMER_INTERNAL_3_TM=55.838 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=0.000 PRIMER_LEFT_3_SELF_ANY=12.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=0.00 PRIMER_LEFT_3_SELF_END=10.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=171 PRIMER_PAIR_4_PENALTY=29.395694 PRIMER_LEFT_4_PENALTY=29.308664 PRIMER_RIGHT_4_PENALTY=0.087031 PRIMER_INTERNAL_4_PENALTY=0.038050 PRIMER_LEFT_4_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGG PRIMER_RIGHT_4_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_4_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_LEFT_4=37,24 PRIMER_RIGHT_4=173,24 PRIMER_INTERNAL_4=61,25 PRIMER_LEFT_4_TM=89.309 PRIMER_RIGHT_4_TM=59.913 PRIMER_INTERNAL_4_TM=55.838 PRIMER_LEFT_4_GC_PERCENT=100.000 PRIMER_RIGHT_4_GC_PERCENT=37.500 PRIMER_INTERNAL_4_GC_PERCENT=0.000 PRIMER_LEFT_4_SELF_ANY=0.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=0.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=12.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=1.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=137 = SEQUENCE_ID=boundary_test_22 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_INTERNAL_MIN_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_INTERNAL_MAX_SELF_ANY=100 PRIMER_INTERNAL_MAX_SELF_END=100 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_INTERNAL_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_PICK_INTERNAL_OLIGO=1 P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_23 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=1,1 P3_COMMENT=should succeed with warning (internal oligo excluded region outside of included region) PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_24 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=2,1 P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_25 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=0,3 P3_COMMENT=should succeed with warning (internal oligo excluded region outside of included region) PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_26 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=29,1 P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_27 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=30,1 P3_COMMENT=should fail w/ no legal internal oligo PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_28 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=54,1 P3_COMMENT=should fail w/ no legal internal oligo PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_29 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=55,1 P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_30 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=81,1 P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_31 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=82,1 P3_COMMENT=should generate warning (external oligo excluded region outside of included region) PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_32 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=81,2 P3_COMMENT=should generate warning (external oligo excluded region outside of included region) PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_33 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 P3_COMMENT=should succeed (testing number of internal oligo excluded regions) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_34 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 P3_COMMENT=should fail (testing number of internal oligo excluded regions) PRIMER_ERROR=Too many elements for tag SEQUENCE_INTERNAL_EXCLUDED_REGION = primer3-2.3.6/test/th-w-other-tasks-settings.txt0000664000077300007730000000466112231156245020564 0ustar ioanaioanaPrimer3 File - http://primer3.sourceforge.net P3_FILE_TYPE=settings PRIMER_FIRST_BASE_INDEX=1 PRIMER_TASK=pick_pcr_primers P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY=../../src/humrep_and_simple.txt PRIMER_PRODUCT_SIZE_RANGE=300-400 401-500 200-299 501-600 100-199 601-700 701-850 851-1001 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00 # PRIMER_MAX_SELF_ANY_TH=47.00 PRIMER_MAX_SELF_END_TH=47.00 PRIMER_MAX_HAIRPIN_TH=47.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.00 PRIMER_PAIR_MAX_COMPL_END_TH=47.00 # # PRIMER_MAX_TEMPLATE_MISPRIMING=12.00 # PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=20.00 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=80.00 PRIMER_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_MAX_SIZE=23 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=59.0 PRIMER_MAX_TM=63.0 PRIMER_PAIR_MAX_DIFF_TM=5.0 # PRIMER_TM_FORMULA=1 means use the SantaLucia parameters PRIMER_TM_FORMULA=1 PRIMER_SALT_MONOVALENT=50.0 # PRIMER_SALT_CORRECTIONS=1 means use the salt correction in SantaLucia et al 1998 PRIMER_SALT_CORRECTIONS=1 # Millimolar conc of MgCl+2 PRIMER_SALT_DIVALENT=1.5 # Millimolar conc of dNTPs PRIMER_DNTP_CONC=0.6 PRIMER_DNA_CONC=50.0 PRIMER_THERMODYNAMIC_PARAMETERS_PATH=../primer3_config/ PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_LOWERCASE_MASKING=0 # PRIMER_MIN_GC=20.0 PRIMER_MAX_GC=80.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_POLY_X=5 PRIMER_OUTSIDE_PENALTY=0 PRIMER_GC_CLAMP=0 PRIMER_LIBERAL_BASE=1 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_WT_SELF_END_TH=0.0 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_WT_NUM_NS=0.0 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_WT_END_QUAL=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_END_TH=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_PICK_ANYWAY=0 = primer3-2.3.6/test/primer3web_v0_4_0_default_settings_input0000664000077300007730000000304612231156245022754 0ustar ioanaioanaSEQUENCE_ID=test_settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag = primer3-2.3.6/test/th-w-other-tasks_list_last/0000775000077300007730000000000012231156372020235 5ustar ioanaioanaprimer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_2.for0000664000077300007730000000626012231156244030034 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- lib qual- # sequence start ln N GC% Tm any_th end_th pin sim lity 0 ATTCCAAAACGCAAAGCACG 118 20 0 45.00 58.514 0.00 0.00 0.00 10.00 0.486 1 CAAAACGCAAAGCACGCTC 122 19 0 52.63 59.161 0.00 0.00 0.00 10.00 1.161 2 TATTCCAAAACGCAAAGCACG 117 21 0 42.86 58.344 0.00 0.00 0.00 10.00 1.656 3 TGACCTGATAGAAACGCCAAC 53 21 0 47.62 58.305 0.00 0.00 0.00 10.00 1.695 4 AACGCCAACTCACTGTATGA 65 20 0 45.00 57.168 0.00 0.00 35.45 11.00 1.832 5 AGAAACGCCAACTCACTGTA 62 20 0 45.00 57.097 0.00 0.00 0.00 11.00 1.903 6 TAGAAACGCCAACTCACTGT 61 20 0 45.00 57.097 0.00 0.00 0.00 11.00 1.903 7 TTCCAAAACGCAAAGCACG 119 19 0 47.37 58.049 0.00 0.00 0.00 10.00 1.951 8 CCTGATAGAAACGCCAACTCA 56 21 0 47.62 58.027 0.00 0.00 0.00 10.00 1.973 9 ACCTGATAGAAACGCCAACTC 55 21 0 47.62 58.026 0.00 0.00 0.00 10.00 1.974 10 GACCTGATAGAAACGCCAACT 54 21 0 47.62 58.026 0.00 0.00 0.00 10.00 1.974 11 AAACGCCAACTCACTGTATGA 64 21 0 42.86 57.880 0.00 0.00 35.45 12.00 2.120 12 TTCCAAAACGCAAAGCACGC 119 20 0 50.00 61.143 0.00 0.00 0.00 9.00 2.143 13 AAAACGCAAAGCACGCTCC 123 19 0 52.63 60.299 0.00 0.00 0.00 9.00 2.299 14 AGAAACGCCAACTCACTGTAT 62 21 0 42.86 57.597 0.00 0.00 0.00 11.00 2.403 15 ATAGAAACGCCAACTCACTGT 60 21 0 42.86 57.597 0.00 0.00 0.00 11.00 2.403 16 GAAACGCCAACTCACTGTATG 63 21 0 47.62 57.544 0.00 0.00 0.00 12.00 2.456 17 CAAAACGCAAAGCACGCTCC 122 20 0 55.00 61.540 0.00 0.00 0.00 9.00 2.540 18 CCAAAACGCAAAGCACGCTC 121 20 0 55.00 61.540 0.00 0.00 0.00 10.00 2.540 19 TCCAAAACGCAAAGCACGC 120 19 0 52.63 60.588 0.00 0.00 0.00 9.00 2.588 20 CCAAAACGCAAAGCACGCT 121 19 0 52.63 60.589 0.00 0.00 0.00 9.00 2.589 21 ATGACCTGATAGAAACGCCAA 52 21 0 42.86 57.373 0.00 0.00 0.00 11.00 2.627 22 TGATAGAAACGCCAACTCACT 58 21 0 42.86 57.322 0.00 0.00 0.00 11.00 2.678 23 AGAAACGCCAACTCACTGT 62 19 0 47.37 57.219 0.00 0.00 0.00 11.00 2.781 24 TCCAAAACGCAAAGCACGCT 120 20 0 50.00 61.999 0.00 0.00 0.00 9.00 2.999 25 GATAGAAACGCCAACTCACTG 59 21 0 47.62 57.001 0.00 0.00 0.00 12.00 2.999 26 CTGATAGAAACGCCAACTCAC 57 21 0 47.62 57.001 0.00 0.00 0.00 10.00 2.999 27 ATTCCAAAACGCAAAGCACGC 118 21 0 47.62 61.455 0.00 0.00 0.00 9.00 3.455 28 AAAACGCAAAGCACGCTCCC 123 20 0 55.00 62.678 0.00 0.00 0.00 9.00 3.678 29 GCAAAGCACGCTCCCGATC 128 19 0 63.16 62.101 0.00 0.00 38.29 10.00 4.101 30 AAACGCAAAGCACGCTCCC 124 19 0 57.89 62.194 0.00 0.00 0.00 9.00 4.194 31 TTCCAAAACGCAAAGCACGCT 119 21 0 47.62 62.473 0.00 0.00 0.00 9.00 4.473 32 TCCAAAACGCAAAGCACGCTC 120 21 0 52.38 62.837 0.00 0.00 0.00 10.00 4.837 primer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_1.rev0000664000077300007730000000655112231156244030044 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- lib qual- # sequence start ln N GC% Tm any_th end_th pin sim lity 0 GGGATCGAATGGCCCACTAA 80 20 0 55.00 59.528 10.16 0.00 32.36 10.00 0.528 1 AGCTATCGATTTGGGTCGGG 97 20 0 55.00 59.607 0.00 0.00 41.74 11.00 0.607 2 GCTATCGATTTGGGTCGGGA 96 20 0 55.00 59.608 1.64 0.00 41.74 12.00 0.608 3 TGCATCGTCAGTGTCATGTC 30 20 0 50.00 58.286 0.00 0.00 0.00 12.00 0.714 4 ATCGATTTGGGTCGGGATCG 93 20 0 55.00 59.966 0.00 0.00 41.74 12.00 0.966 5 GTCTGCATCGTCAGTGTCAT 33 20 0 50.00 57.998 0.00 0.00 0.00 12.00 1.002 6 GCATCGTCAGTGTCATGTCT 29 20 0 50.00 57.998 0.00 0.00 0.00 11.00 1.002 7 GGGATCGAATGGCCCACTA 80 19 0 57.89 58.858 10.16 0.00 32.36 10.00 1.142 8 CGTAGCTATCGATTTGGGTCG 100 21 0 52.38 58.683 9.28 4.23 42.74 11.00 1.317 9 TAGCTATCGATTTGGGTCGGG 98 21 0 52.38 59.383 0.00 0.00 41.74 11.00 1.383 10 TCGATTTGGGTCGGGATCG 92 19 0 57.89 59.560 0.00 0.00 41.74 12.00 1.560 11 CGATTTGGGTCGGGATCGA 91 19 0 57.89 59.560 0.00 0.00 43.86 12.00 1.560 12 GGATCGAATGGCCCACTAAT 79 20 0 50.00 57.431 0.00 0.00 0.00 11.00 1.569 13 GTAGCTATCGATTTGGGTCGG 99 21 0 52.38 58.326 0.00 0.00 41.74 11.00 1.674 14 TGCATCGTCAGTGTCATGTCT 30 21 0 47.62 59.727 1.94 0.00 0.00 12.00 1.727 15 TATCGATTTGGGTCGGGATCG 94 21 0 52.38 59.729 1.95 0.00 41.74 12.00 1.729 16 GCTATCGATTTGGGTCGGG 96 19 0 57.89 58.086 1.64 0.00 41.74 11.00 1.914 17 GGGATCGAATGGCCCACTAAT 80 21 0 52.38 59.926 10.16 0.00 32.36 11.00 1.926 18 CTATCGATTTGGGTCGGGATC 95 21 0 52.38 58.039 0.00 0.00 41.74 12.00 1.961 19 GCTATCGATTTGGGTCGGGAT 96 21 0 52.38 59.998 1.64 0.00 41.74 12.00 1.998 20 TCGATTTGGGTCGGGATCGA 92 20 0 55.00 61.039 5.62 4.35 43.86 12.00 2.039 21 GCATCGTCAGTGTCATGTCTA 29 21 0 47.62 57.849 0.00 0.00 0.00 11.00 2.151 22 GTCTGCATCGTCAGTGTCA 33 19 0 52.63 57.503 0.00 0.00 0.00 12.00 2.497 23 TCGTAGCTATCGATTTGGGTC 101 21 0 47.62 57.362 14.37 0.00 0.00 11.00 2.638 24 GGATCGAATGGCCCACTAATA 79 21 0 47.62 57.304 0.00 0.00 0.00 11.00 2.696 25 CGGGATCGAATGGCCCACTA 81 20 0 60.00 61.754 14.03 0.00 39.00 10.00 2.754 26 TCGTCAGTGTCATGTCTACTG 26 21 0 47.62 57.168 15.66 15.66 0.00 11.00 2.832 27 CGTCAGTGTCATGTCTACTGA 25 21 0 47.62 57.168 19.25 17.68 0.00 11.00 2.832 28 TGCATCGTCAGTGTCATGT 30 19 0 47.37 57.087 3.35 0.00 0.00 12.00 2.913 29 AGCTATCGATTTGGGTCGG 97 19 0 52.63 57.011 0.00 0.00 41.74 10.00 2.989 30 AGCTATCGATTTGGGTCGGGA 97 21 0 52.38 61.033 0.00 0.00 41.74 12.00 3.033 31 ATCGATTTGGGTCGGGATCGA 93 21 0 52.38 61.368 7.64 7.64 43.86 12.00 3.368 32 TCGGGATCGAATGGCCCAC 82 19 0 63.16 62.102 14.03 0.00 43.03 10.00 4.102 33 CGGGATCGAATGGCCCACT 81 19 0 63.16 62.107 14.03 0.00 39.00 10.00 4.107 34 CGGGATCGAATGGCCCACTAA 81 21 0 57.14 62.250 14.03 0.00 39.00 10.00 4.250 primer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_4.int0000664000077300007730000001452112231156244030041 0ustar ioanaioanaACCEPTABLE INTERNAL OLIGOS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 AACGCAAAGCACGCTCCCGA 125 20 0 60.00 59.906 7.82 0.00 36.42 0.094 1 ACGCAAAGCACGCTCCCGAT 126 20 0 60.00 59.699 7.82 0.00 36.42 0.301 2 AAACGCAAAGCACGCTCCCG 124 20 0 60.00 59.100 1.50 1.50 41.14 0.900 3 CGCAAAGCACGCTCCCGATC 127 20 0 65.00 59.043 0.00 0.00 36.42 0.957 4 AACGCAAAGCACGCTCCCGAT 125 21 0 57.14 59.982 7.82 0.00 36.42 1.018 5 AAACGCAAAGCACGCTCCCGA 124 21 0 57.14 60.179 7.82 0.00 36.42 1.179 6 ACGCAAAGCACGCTCCCGATC 126 21 0 61.90 60.373 7.82 0.00 36.42 1.373 7 ACGCAAAGCACGCTCCCGA 126 19 0 63.16 59.606 7.82 0.00 36.42 1.394 8 AAAACGCAAAGCACGCTCCCG 123 21 0 57.14 59.405 1.50 1.50 41.14 1.595 9 CCAAAACGCAAAGCACGCTCCC 121 22 0 59.09 59.992 0.00 0.00 0.00 2.008 10 AACGCAAAGCACGCTCCCG 125 19 0 63.16 58.764 1.50 1.50 41.14 2.236 11 AAACGCAAAGCACGCTCCCGAT 124 22 0 54.55 60.240 7.82 0.00 36.42 2.240 12 CAAAACGCAAAGCACGCTCCCG 122 22 0 59.09 60.299 1.50 1.50 41.14 2.299 13 AAAACGCAAAGCACGCTCCCGA 123 22 0 54.55 60.427 7.82 0.00 36.42 2.427 14 AACGCAAAGCACGCTCCCGATC 125 22 0 59.09 60.611 7.82 0.00 36.42 2.611 15 CGCAAAGCACGCTCCCGAT 127 19 0 63.16 58.260 0.00 0.00 36.42 2.740 16 TCCAAAACGCAAAGCACGCTCC 120 22 0 54.55 59.120 0.00 0.00 0.00 2.880 17 CAAAACGCAAAGCACGCTCCC 122 21 0 57.14 58.061 0.00 0.00 0.00 2.939 18 CCAAAACGCAAAGCACGCTCC 121 21 0 57.14 58.061 0.00 0.00 0.00 2.939 19 AAAACGCAAAGCACGCTCCCGAT 123 23 0 52.17 60.477 7.82 0.00 36.42 3.477 20 TTCCAAAACGCAAAGCACGCTCC 119 23 0 52.17 59.401 0.00 0.00 0.00 3.599 21 ACGCAAAGCACGCTCCCG 126 18 0 66.67 58.394 1.50 1.50 41.14 3.606 22 AAACGCAAAGCACGCTCCCGATC 124 23 0 56.52 60.829 7.82 0.00 36.42 3.829 23 TCCAAAACGCAAAGCACGCTC 120 21 0 52.38 57.135 0.00 0.00 0.00 3.865 24 CGCAAAGCACGCTCCCGA 127 18 0 66.67 58.095 0.00 0.00 36.42 3.905 25 TCCAAAACGCAAAGCACGCTCCC 120 23 0 56.52 60.955 0.00 0.00 0.00 3.955 26 CAAAACGCAAAGCACGCTCCCGA 122 23 0 56.52 61.240 7.82 0.00 36.42 4.240 27 TTCCAAAACGCAAAGCACGCTC 119 22 0 50.00 57.513 0.00 0.00 0.00 4.487 28 ATTCCAAAACGCAAAGCACGCTCC 118 24 0 50.00 59.486 0.00 0.00 0.00 4.514 29 AAAACGCAAAGCACGCTCCCGATC 123 24 0 54.17 61.030 0.00 0.00 36.42 5.030 30 CCAAAACGCAAAGCACGCTCCCG 121 23 0 60.87 62.052 1.50 1.50 41.14 5.052 31 TTCCAAAACGCAAAGCACGCTCCC 119 24 0 54.17 61.152 0.00 0.00 0.00 5.152 32 CAAAACGCAAAGCACGCTCCCGAT 122 24 0 54.17 61.257 7.82 0.00 36.42 5.257 33 ATTCCAAAACGCAAAGCACGCTC 118 23 0 47.83 57.674 0.00 0.00 0.00 5.326 34 TATTCCAAAACGCAAAGCACGCTCC 117 25 0 48.00 59.068 0.00 0.00 0.00 5.932 35 ATTCCAAAACGCAAAGCACGCTCCC 118 25 0 52.00 61.171 0.00 0.00 0.00 6.171 36 CTATTCCAAAACGCAAAGCACGCTCC 116 26 0 50.00 59.646 0.00 0.00 0.00 6.354 37 TATTCCAAAACGCAAAGCACGCTC 117 24 0 45.83 57.311 0.00 0.00 0.00 6.689 38 TCTATTCCAAAACGCAAAGCACGC 115 24 0 45.83 57.311 0.00 0.00 0.00 6.689 39 CTATTCCAAAACGCAAAGCACGCT 116 24 0 45.83 57.305 0.00 0.00 0.00 6.695 40 TATTCCAAAACGCAAAGCACGCTCCC 117 26 0 50.00 60.707 0.00 0.00 0.00 6.707 41 TCTATTCCAAAACGCAAAGCACGCT 115 25 0 44.00 58.276 0.00 0.00 0.00 6.724 42 CAAAACGCAAAGCACGCTCCCGATC 122 25 0 56.00 61.744 0.00 0.00 36.42 6.744 43 GTCTATTCCAAAACGCAAAGCACGC 114 25 0 48.00 58.214 0.00 0.00 0.00 6.786 44 GTCTATTCCAAAACGCAAAGCACGCT 114 26 0 46.15 59.111 0.00 0.00 0.00 6.889 45 AGTCTATTCCAAAACGCAAAGCACGC 113 26 0 46.15 59.111 0.00 0.00 0.00 6.889 46 CCAAAACGCAAAGCACGCTCCCGA 121 24 0 58.33 62.910 7.82 0.00 36.42 6.910 47 TCCAAAACGCAAAGCACGCTCCCG 120 24 0 58.33 62.910 1.50 1.50 41.14 6.910 48 CTATTCCAAAACGCAAAGCACGCTC 116 25 0 48.00 57.992 0.00 0.00 0.00 7.008 49 AGTCTATTCCAAAACGCAAAGCACGCT 113 27 0 44.44 59.965 0.00 0.00 0.00 7.035 50 TCTATTCCAAAACGCAAAGCACGCTC 115 26 0 46.15 58.898 0.00 0.00 0.00 7.102 51 GTCTATTCCAAAACGCAAAGCACGCTC 114 27 0 48.15 59.666 0.00 0.00 0.00 7.334 52 TCTATTCCAAAACGCAAAGCACGCTCC 115 27 0 48.15 60.481 0.00 0.00 0.00 7.481 53 AAGTCTATTCCAAAACGCAAAGCACGC 112 27 0 44.44 59.353 0.00 0.00 0.00 7.647 54 ACCTGATAGAAACGCCAACTCACTGT 55 26 0 46.15 58.256 0.00 0.00 0.00 7.744 55 CCAAAACGCAAAGCACGCTCCCGAT 121 25 0 56.00 62.867 7.82 0.00 36.42 7.867 56 TGACCTGATAGAAACGCCAACTCAC 53 25 0 48.00 57.083 0.00 0.00 0.00 7.917 57 CCTGATAGAAACGCCAACTCACTGT 56 25 0 48.00 57.077 0.00 0.00 0.00 7.923 58 ACCTGATAGAAACGCCAACTCACTG 55 25 0 48.00 57.077 0.00 0.00 0.00 7.923 59 TGACCTGATAGAAACGCCAACTCACT 53 26 0 46.15 58.036 0.00 0.00 0.00 7.964 60 TGACCTGATAGAAACGCCAACTCACTG 53 27 0 48.15 58.868 0.00 0.00 0.00 8.132 61 GACCTGATAGAAACGCCAACTCACTGT 54 27 0 48.15 58.868 0.00 0.00 0.00 8.132 62 CTATTCCAAAACGCAAAGCACGCTCCC 116 27 0 51.85 61.193 0.00 0.00 0.00 8.193 63 GACCTGATAGAAACGCCAACTCACTG 54 26 0 50.00 57.762 0.00 0.00 0.00 8.238 64 ATGACCTGATAGAAACGCCAACTCAC 52 26 0 46.15 57.245 0.00 0.00 0.00 8.755 65 AAGTCTATTCCAAAACGCAAAGCACG 112 26 0 42.31 57.241 0.00 0.00 0.00 8.759 66 ATGACCTGATAGAAACGCCAACTCACT 52 27 0 44.44 58.161 0.00 0.00 0.00 8.839 67 ATTCCAAAACGCAAAGCACGCTCCCG 118 26 0 53.85 62.978 1.50 1.50 41.14 8.978 68 ACCTGATAGAAACGCCAACTCACTGTA 55 27 0 44.44 57.900 0.00 0.00 0.00 9.100 69 AAAGTCTATTCCAAAACGCAAAGCACG 111 27 0 40.74 57.551 0.00 0.00 0.00 9.449 70 TATTCCAAAACGCAAAGCACGCTCCCG 117 27 0 51.85 62.472 1.50 1.50 41.14 9.472 71 CGTTGTCAAAGTCTATTCCAAAACGCA 104 27 0 40.74 57.087 0.00 0.00 0.00 9.913 72 ACGTTGTCAAAGTCTATTCCAAAACGC 103 27 0 40.74 57.086 0.00 0.00 0.00 9.914 primer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_3.for0000664000077300007730000001160412231156244030033 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- lib qual- # sequence start ln N GC% Tm any_th end_th pin sim lity 0 TTAGTGGGCCATTCGATCCC 61 20 0 55.00 59.528 5.82 5.82 0.00 9.00 0.528 1 CCCGACCCAAATCGATAGCT 78 20 0 55.00 59.607 0.00 0.00 45.13 9.00 0.607 2 TCCCGACCCAAATCGATAGC 77 20 0 55.00 59.608 0.00 0.00 45.13 10.00 0.608 3 GACATGACACTGACGATGCA 11 20 0 50.00 58.286 0.00 0.00 0.00 12.00 0.714 4 CGATCCCGACCCAAATCGAT 74 20 0 55.00 59.966 0.00 0.00 45.13 11.00 0.966 5 CATTCGATCCCGACCCAAAT 70 20 0 50.00 58.033 0.00 0.00 45.50 11.00 0.967 6 CATGACACTGACGATGCAGA 13 20 0 50.00 58.000 10.65 0.00 0.00 12.00 1.000 7 ATGACACTGACGATGCAGAC 14 20 0 50.00 57.998 4.19 0.00 0.00 12.00 1.002 8 AGACATGACACTGACGATGC 10 20 0 50.00 57.998 0.00 0.00 0.00 11.00 1.002 9 CCATTCGATCCCGACCCAAA 69 20 0 55.00 60.107 0.00 0.00 45.50 10.00 1.107 10 CATTCGATCCCGACCCAAATC 70 21 0 52.38 59.126 0.00 0.00 45.50 12.00 1.126 11 TAGTGGGCCATTCGATCCC 62 19 0 57.89 58.858 5.82 5.82 0.00 9.00 1.142 12 TTCGATCCCGACCCAAATCG 72 20 0 55.00 60.179 0.00 0.00 45.50 11.00 1.179 13 ATTCGATCCCGACCCAAATC 71 20 0 50.00 57.744 0.00 0.00 45.50 12.00 1.256 14 CGACCCAAATCGATAGCTACG 80 21 0 52.38 58.683 8.77 8.77 45.13 10.00 1.317 15 CCCGACCCAAATCGATAGCTA 78 21 0 52.38 59.383 0.00 0.00 45.13 10.00 1.383 16 CCATTCGATCCCGACCCAA 69 19 0 57.89 59.475 0.00 0.00 45.50 9.00 1.475 17 CGATCCCGACCCAAATCGA 74 19 0 57.89 59.560 0.00 0.00 45.13 10.00 1.560 18 TCGATCCCGACCCAAATCG 73 19 0 57.89 59.560 0.00 0.00 40.30 11.00 1.560 19 ATTAGTGGGCCATTCGATCC 60 20 0 50.00 57.431 0.00 0.00 0.00 9.00 1.569 20 CCGACCCAAATCGATAGCTAC 79 21 0 52.38 58.326 0.00 0.00 45.13 11.00 1.674 21 AGACATGACACTGACGATGCA 10 21 0 47.62 59.727 0.00 0.00 0.00 12.00 1.727 22 CGATCCCGACCCAAATCGATA 74 21 0 52.38 59.729 0.00 0.00 45.13 10.00 1.729 23 CCCGACCCAAATCGATAGC 78 19 0 57.89 58.086 0.00 0.00 45.13 10.00 1.914 24 ATTAGTGGGCCATTCGATCCC 60 21 0 52.38 59.926 5.82 5.82 0.00 9.00 1.926 25 GATCCCGACCCAAATCGATAG 75 21 0 52.38 58.039 0.00 0.00 45.13 10.00 1.961 26 ATCCCGACCCAAATCGATAGC 76 21 0 52.38 59.998 0.00 0.00 45.13 10.00 1.998 27 TCGATCCCGACCCAAATCGA 73 20 0 55.00 61.039 4.17 2.87 45.13 10.00 2.039 28 TAGACATGACACTGACGATGC 9 21 0 47.62 57.849 3.11 0.00 0.00 11.00 2.151 29 CATTCGATCCCGACCCAAA 70 19 0 52.63 57.530 0.00 0.00 45.50 10.00 2.470 30 CCATTCGATCCCGACCCAAAT 69 21 0 52.38 60.477 0.00 0.00 45.50 11.00 2.477 31 TGACACTGACGATGCAGAC 15 19 0 52.63 57.503 4.19 0.00 0.00 11.00 2.497 32 ATTCGATCCCGACCCAAATCG 71 21 0 52.38 60.540 0.00 0.00 45.50 11.00 2.540 33 GACCCAAATCGATAGCTACGA 81 21 0 47.62 57.362 16.85 16.85 43.65 11.00 2.638 34 TATTAGTGGGCCATTCGATCC 59 21 0 47.62 57.304 0.00 0.00 0.00 9.00 2.696 35 TAGTGGGCCATTCGATCCCG 62 20 0 60.00 61.754 7.70 0.00 37.07 9.00 2.754 36 TTCGATCCCGACCCAAATC 72 19 0 52.63 57.227 0.00 0.00 45.50 12.00 2.773 37 CAGTAGACATGACACTGACGA 6 21 0 47.62 57.168 16.46 0.00 0.00 11.00 2.832 38 TCAGTAGACATGACACTGACG 5 21 0 47.62 57.168 20.80 6.93 0.00 10.00 2.832 39 ACATGACACTGACGATGCA 12 19 0 47.37 57.087 0.00 0.00 0.00 12.00 2.913 40 CCGACCCAAATCGATAGCT 79 19 0 52.63 57.011 0.00 0.00 45.13 9.00 2.989 41 TCCCGACCCAAATCGATAGCT 77 21 0 52.38 61.033 0.00 0.00 45.13 10.00 3.033 42 TCGATCCCGACCCAAATCGAT 73 21 0 52.38 61.368 3.83 0.00 45.13 11.00 3.368 43 TTCGATCCCGACCCAAATCGA 72 21 0 52.38 61.568 4.17 3.06 45.50 10.00 3.568 44 GCCATTCGATCCCGACCCAA 68 20 0 60.00 62.603 0.00 0.00 45.50 10.00 3.603 45 GCCATTCGATCCCGACCCA 68 19 0 63.16 62.102 0.00 0.00 45.50 10.00 4.102 46 TGGGCCATTCGATCCCGAC 65 19 0 63.16 62.102 7.70 0.00 45.50 9.00 4.102 47 GTGGGCCATTCGATCCCGA 64 19 0 63.16 62.102 5.82 0.00 43.96 9.00 4.102 48 AGTGGGCCATTCGATCCCG 63 19 0 63.16 62.107 7.70 0.00 37.07 9.00 4.107 49 TTAGTGGGCCATTCGATCCCG 61 21 0 57.14 62.250 7.70 0.00 37.07 9.00 4.250 50 GGCCATTCGATCCCGACCC 67 19 0 68.42 62.827 0.00 0.00 45.50 9.00 4.827 51 GGGCCATTCGATCCCGACC 66 19 0 68.42 62.827 4.39 0.00 45.50 9.00 4.827 primer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_4.for0000664000077300007730000000626012231156244030036 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- lib qual- # sequence start ln N GC% Tm any_th end_th pin sim lity 0 ATTCCAAAACGCAAAGCACG 118 20 0 45.00 58.514 0.00 0.00 0.00 10.00 0.486 1 CAAAACGCAAAGCACGCTC 122 19 0 52.63 59.161 0.00 0.00 0.00 10.00 1.161 2 TATTCCAAAACGCAAAGCACG 117 21 0 42.86 58.344 0.00 0.00 0.00 10.00 1.656 3 TGACCTGATAGAAACGCCAAC 53 21 0 47.62 58.305 0.00 0.00 0.00 10.00 1.695 4 AACGCCAACTCACTGTATGA 65 20 0 45.00 57.168 0.00 0.00 35.45 11.00 1.832 5 AGAAACGCCAACTCACTGTA 62 20 0 45.00 57.097 0.00 0.00 0.00 11.00 1.903 6 TAGAAACGCCAACTCACTGT 61 20 0 45.00 57.097 0.00 0.00 0.00 11.00 1.903 7 TTCCAAAACGCAAAGCACG 119 19 0 47.37 58.049 0.00 0.00 0.00 10.00 1.951 8 CCTGATAGAAACGCCAACTCA 56 21 0 47.62 58.027 0.00 0.00 0.00 10.00 1.973 9 ACCTGATAGAAACGCCAACTC 55 21 0 47.62 58.026 0.00 0.00 0.00 10.00 1.974 10 GACCTGATAGAAACGCCAACT 54 21 0 47.62 58.026 0.00 0.00 0.00 10.00 1.974 11 AAACGCCAACTCACTGTATGA 64 21 0 42.86 57.880 0.00 0.00 35.45 12.00 2.120 12 TTCCAAAACGCAAAGCACGC 119 20 0 50.00 61.143 0.00 0.00 0.00 9.00 2.143 13 AAAACGCAAAGCACGCTCC 123 19 0 52.63 60.299 0.00 0.00 0.00 9.00 2.299 14 AGAAACGCCAACTCACTGTAT 62 21 0 42.86 57.597 0.00 0.00 0.00 11.00 2.403 15 ATAGAAACGCCAACTCACTGT 60 21 0 42.86 57.597 0.00 0.00 0.00 11.00 2.403 16 GAAACGCCAACTCACTGTATG 63 21 0 47.62 57.544 0.00 0.00 0.00 12.00 2.456 17 CAAAACGCAAAGCACGCTCC 122 20 0 55.00 61.540 0.00 0.00 0.00 9.00 2.540 18 CCAAAACGCAAAGCACGCTC 121 20 0 55.00 61.540 0.00 0.00 0.00 10.00 2.540 19 TCCAAAACGCAAAGCACGC 120 19 0 52.63 60.588 0.00 0.00 0.00 9.00 2.588 20 CCAAAACGCAAAGCACGCT 121 19 0 52.63 60.589 0.00 0.00 0.00 9.00 2.589 21 ATGACCTGATAGAAACGCCAA 52 21 0 42.86 57.373 0.00 0.00 0.00 11.00 2.627 22 TGATAGAAACGCCAACTCACT 58 21 0 42.86 57.322 0.00 0.00 0.00 11.00 2.678 23 AGAAACGCCAACTCACTGT 62 19 0 47.37 57.219 0.00 0.00 0.00 11.00 2.781 24 TCCAAAACGCAAAGCACGCT 120 20 0 50.00 61.999 0.00 0.00 0.00 9.00 2.999 25 GATAGAAACGCCAACTCACTG 59 21 0 47.62 57.001 0.00 0.00 0.00 12.00 2.999 26 CTGATAGAAACGCCAACTCAC 57 21 0 47.62 57.001 0.00 0.00 0.00 10.00 2.999 27 ATTCCAAAACGCAAAGCACGC 118 21 0 47.62 61.455 0.00 0.00 0.00 9.00 3.455 28 AAAACGCAAAGCACGCTCCC 123 20 0 55.00 62.678 0.00 0.00 0.00 9.00 3.678 29 GCAAAGCACGCTCCCGATC 128 19 0 63.16 62.101 0.00 0.00 38.29 10.00 4.101 30 AAACGCAAAGCACGCTCCC 124 19 0 57.89 62.194 0.00 0.00 0.00 9.00 4.194 31 TTCCAAAACGCAAAGCACGCT 119 21 0 47.62 62.473 0.00 0.00 0.00 9.00 4.473 32 TCCAAAACGCAAAGCACGCTC 120 21 0 52.38 62.837 0.00 0.00 0.00 10.00 4.837 primer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_1.int0000664000077300007730000004137112231156244030041 0ustar ioanaioanaACCEPTABLE INTERNAL OLIGOS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 GGGCCATTCGATCCCGACCC 66 20 0 70.00 59.273 2.18 0.00 41.60 0.727 1 GTGGGCCATTCGATCCCGACC 64 21 0 66.67 59.583 2.53 0.00 41.60 1.417 2 GGCCATTCGATCCCGACCCA 67 20 0 65.00 58.561 0.00 0.00 41.60 1.439 3 TGGGCCATTCGATCCCGACC 65 20 0 65.00 58.561 4.28 0.00 41.60 1.439 4 GGGCCATTCGATCCCGACCCA 66 21 0 66.67 60.650 2.16 0.00 41.60 1.650 5 TGGGCCATTCGATCCCGACCC 65 21 0 66.67 60.650 4.28 0.51 41.60 1.650 6 GGCCATTCGATCCCGACCCAA 67 21 0 61.90 58.904 0.00 0.00 41.60 2.096 7 AGTGGGCCATTCGATCCCGAC 63 21 0 61.90 58.640 3.57 3.02 41.60 2.360 8 AGTGGGCCATTCGATCCCGA 63 20 0 60.00 57.544 3.28 0.00 41.96 2.456 9 GTGGGCCATTCGATCCCGAC 64 20 0 65.00 57.489 0.97 0.00 41.60 2.511 10 AGTGGGCCATTCGATCCCGACC 63 22 0 63.64 60.627 5.61 0.00 41.60 2.627 11 GGCCATTCGATCCCGACCCAAA 67 22 0 59.09 59.216 0.00 0.00 41.60 2.784 12 GGGCCATTCGATCCCGACCCAA 66 22 0 63.64 60.885 2.16 0.00 41.60 2.885 13 TAGTGGGCCATTCGATCCCGACC 62 23 0 60.87 60.114 5.61 0.00 41.60 3.114 14 GTGGGCCATTCGATCCCGACCC 64 22 0 68.18 61.511 2.53 0.25 41.60 3.511 15 GGCCATTCGATCCCGACCCAAAT 67 23 0 56.52 59.315 0.00 0.00 41.60 3.685 16 TAGTGGGCCATTCGATCCCGAC 62 22 0 59.09 58.192 3.57 3.02 41.60 3.808 17 GCCATTCGATCCCGACCCAAA 68 21 0 57.14 57.174 0.00 0.00 41.60 3.826 18 ACACACACACACAGCACACAGGT 37 23 0 52.17 59.135 0.00 0.00 0.00 3.865 19 TAGTGGGCCATTCGATCCCGA 62 21 0 57.14 57.119 0.00 0.00 41.96 3.881 20 TGGGCCATTCGATCCCGACCCA 65 22 0 63.64 61.937 8.58 6.38 42.67 3.937 21 GGCCATTCGATCCCGACCC 67 19 0 68.42 57.043 0.00 0.00 41.60 3.957 22 GGGCCATTCGATCCCGACC 66 19 0 68.42 57.043 2.18 0.00 41.60 3.957 23 CACACACACACACAGCACACAGGT 36 24 0 54.17 60.001 0.00 0.00 0.00 4.001 24 ACACACACACACACAGCACACAGG 35 24 0 54.17 60.001 0.00 0.00 0.00 4.001 25 GGCCATTCGATCCCGACCCAAATC 67 24 0 58.33 59.942 0.00 0.00 41.60 4.058 26 GGGCCATTCGATCCCGACCCAAA 66 23 0 60.87 61.100 2.16 0.00 41.60 4.100 27 CACACACACACAGCACACAGGT 38 22 0 54.55 57.841 0.00 0.00 0.00 4.159 28 ACACACACACACAGCACACAGG 37 22 0 54.55 57.841 0.00 0.00 0.00 4.159 29 CACACACACACACAGCACACAGG 36 23 0 56.52 58.804 0.00 0.00 0.00 4.196 30 GCCATTCGATCCCGACCCAAATCG 68 24 0 58.33 60.233 0.00 0.00 41.60 4.233 31 TTAGTGGGCCATTCGATCCCGACC 61 24 0 58.33 60.352 5.61 0.00 41.60 4.352 32 TTAGTGGGCCATTCGATCCCGAC 61 23 0 56.52 58.521 3.57 3.02 41.60 4.479 33 TTAGTGGGCCATTCGATCCCGA 61 22 0 54.55 57.516 0.00 0.00 41.96 4.484 34 ACACACACACACACAGCACACA 35 22 0 50.00 57.448 0.00 0.00 0.00 4.552 35 GCCATTCGATCCCGACCCAAAT 68 22 0 54.55 57.361 0.00 0.00 41.60 4.639 36 ACACACACACACACAGCACACAG 35 23 0 52.17 58.191 0.00 0.00 0.00 4.809 37 GCCATTCGATCCCGACCCAAATC 68 23 0 56.52 58.119 0.00 0.00 41.60 4.881 38 TGGGCCATTCGATCCCGACCCAA 65 23 0 60.87 62.108 8.58 0.00 41.29 5.108 39 CCATTCGATCCCGACCCAAATCGA 69 24 0 54.17 58.889 0.00 0.00 41.60 5.111 40 CCATTCGATCCCGACCCAAATCG 69 23 0 56.52 57.889 0.00 0.00 41.60 5.111 41 ACAGCACACAGGTATTAGTGGGCCA 47 25 0 52.00 59.886 8.63 1.50 32.68 5.114 42 GGGCCATTCGATCCCGACCCAAAT 66 24 0 58.33 61.123 2.16 0.00 41.60 5.123 43 AGCACACAGGTATTAGTGGGCCA 49 23 0 52.17 57.732 8.63 1.50 32.68 5.268 44 ACACACACACACAGCACACAGGTA 37 24 0 50.00 58.705 0.00 0.00 0.00 5.295 45 CAGCACACAGGTATTAGTGGGCCA 48 24 0 54.17 58.694 8.63 1.50 32.68 5.306 46 ACAGCACACAGGTATTAGTGGGCC 47 24 0 54.17 58.693 8.63 0.00 32.68 5.307 47 ATTAGTGGGCCATTCGATCCCGA 60 23 0 52.17 57.684 0.00 0.00 41.96 5.316 48 ATTAGTGGGCCATTCGATCCCGAC 60 24 0 54.17 58.642 3.57 3.02 41.60 5.358 49 ATTAGTGGGCCATTCGATCCCGACC 60 25 0 56.00 60.401 5.61 0.00 41.60 5.401 50 CACACACACACACAGCACACAGGTA 36 25 0 52.00 59.560 0.00 0.00 0.00 5.440 51 CACAGCACACAGGTATTAGTGGGCC 46 25 0 56.00 59.556 8.63 0.00 32.68 5.444 52 AGTGGGCCATTCGATCCCGACCC 63 23 0 65.22 62.459 5.61 2.93 41.60 5.459 53 TCGATCCCGACCCAAATCGATAGC 73 24 0 54.17 58.485 0.30 0.00 41.28 5.515 54 CGATCCCGACCCAAATCGATAGCT 74 24 0 54.17 58.481 0.00 0.00 41.28 5.519 55 CGATCCCGACCCAAATCGATAGC 74 23 0 56.52 57.476 0.00 0.00 41.28 5.524 56 CACACACACACAGCACACAGGTA 38 23 0 52.17 57.450 0.00 0.00 0.00 5.550 57 TCGATCCCGACCCAAATCGATAGCT 73 25 0 52.00 59.436 0.30 0.00 41.28 5.564 58 CAGCACACAGGTATTAGTGGGCC 48 23 0 56.52 57.427 8.63 0.00 32.68 5.573 59 GTGGGCCATTCGATCCCGACCCA 64 23 0 65.22 62.698 8.58 6.40 42.67 5.698 60 CCCGACCCAAATCGATAGCTACGA 78 24 0 54.17 58.193 12.68 12.68 41.28 5.807 61 TCCCGACCCAAATCGATAGCTACG 77 24 0 54.17 58.193 5.17 5.17 41.28 5.807 62 CCCGACCCAAATCGATAGCTACG 78 23 0 56.52 57.176 5.17 5.17 41.28 5.824 63 TCCCGACCCAAATCGATAGCTACGA 77 25 0 52.00 59.154 12.68 12.68 41.28 5.846 64 TAGTGGGCCATTCGATCCCGACCC 62 24 0 62.50 61.893 5.61 2.93 41.60 5.893 65 GGTATTAGTGGGCCATTCGATCCCG 57 25 0 56.00 59.049 4.28 0.00 37.07 5.951 66 CATTCGATCCCGACCCAAATCGA 70 23 0 52.17 57.019 0.00 0.00 41.60 5.981 67 CCATTCGATCCCGACCCAAATCGAT 69 25 0 52.00 58.991 0.00 0.00 41.60 6.009 68 ACACAGCACACAGGTATTAGTGGGC 45 25 0 52.00 58.985 5.21 5.21 33.51 6.015 69 TATTAGTGGGCCATTCGATCCCGACC 59 26 0 53.85 59.954 5.61 0.00 41.60 6.046 70 GGTATTAGTGGGCCATTCGATCCCGA 57 26 0 53.85 59.954 0.76 0.00 41.96 6.046 71 AGGTATTAGTGGGCCATTCGATCCCG 56 26 0 53.85 59.953 4.31 0.00 37.07 6.047 72 ACAGCACACAGGTATTAGTGGGCCAT 47 26 0 50.00 59.951 8.63 0.00 32.68 6.049 73 GCACACAGGTATTAGTGGGCCATTCG 50 26 0 53.85 59.904 2.67 0.00 28.81 6.096 74 AGCACACAGGTATTAGTGGGCCAT 49 24 0 50.00 57.886 8.63 0.00 32.68 6.114 75 GCCATTCGATCCCGACCCAAATCGA 68 25 0 56.00 61.125 0.00 0.00 41.60 6.125 76 ACACACACACACACAGCACACAGGT 35 25 0 52.00 61.132 0.00 0.00 0.00 6.132 77 ACACACACACACAGCACACAGGTAT 37 25 0 48.00 58.814 0.00 0.00 0.00 6.186 78 CAGCACACAGGTATTAGTGGGCCAT 48 25 0 52.00 58.804 8.63 0.00 32.68 6.196 79 CACACAGCACACAGGTATTAGTGGGC 44 26 0 53.85 59.796 5.21 5.21 33.51 6.204 80 CACAGCACACAGGTATTAGTGGGC 46 24 0 54.17 57.786 5.21 5.21 33.51 6.214 81 TTCGATCCCGACCCAAATCGATAGC 72 25 0 52.00 58.774 0.00 0.00 41.60 6.226 82 TGGGCCATTCGATCCCGACCCAAA 65 24 0 58.33 62.265 8.58 0.00 41.29 6.265 83 TTCGATCCCGACCCAAATCGATAGCT 72 26 0 50.00 59.681 0.00 0.00 41.60 6.319 84 CACACACACACACAGCACACAGGTAT 36 26 0 50.00 59.634 0.00 0.00 0.00 6.366 85 CACACACACACAGCACACAGGTAT 38 24 0 50.00 57.610 0.00 0.00 0.00 6.390 86 GGGCCATTCGATCCCGACCCAAATC 66 25 0 60.00 61.636 2.16 0.00 41.60 6.636 87 ACACACACACACACAGCACACAGGTA 35 26 0 50.00 60.661 0.00 0.00 0.00 6.661 88 CACAGCACACAGGTATTAGTGGGCCA 46 26 0 53.85 60.665 8.63 1.50 32.68 6.665 89 ACACAGCACACAGGTATTAGTGGGCC 45 26 0 53.85 60.665 8.63 0.00 32.68 6.665 90 CCCGACCCAAATCGATAGCTACGAT 78 25 0 52.00 58.320 14.48 14.48 46.54 6.680 91 ATCCCGACCCAAATCGATAGCTACG 76 25 0 52.00 58.320 5.17 5.17 41.28 6.680 92 TATTAGTGGGCCATTCGATCCCGA 59 24 0 50.00 57.304 0.00 0.00 41.96 6.696 93 GTATTAGTGGGCCATTCGATCCCG 58 24 0 54.17 57.258 4.28 0.00 37.07 6.742 94 TATTAGTGGGCCATTCGATCCCGAC 59 25 0 52.00 58.246 3.57 3.02 41.60 6.754 95 GTATTAGTGGGCCATTCGATCCCGA 58 25 0 52.00 58.246 0.00 0.00 41.96 6.754 96 TCCCGACCCAAATCGATAGCTACGAT 77 26 0 50.00 59.244 14.48 14.48 46.54 6.756 97 ATCCCGACCCAAATCGATAGCTACGA 76 26 0 50.00 59.244 12.68 12.68 41.28 6.756 98 AGCACACAGGTATTAGTGGGCCATT 49 25 0 48.00 58.208 8.63 0.00 32.68 6.792 99 GCACACAGGTATTAGTGGGCCATT 50 24 0 50.00 57.207 2.67 0.00 28.81 6.793 100 CATTCGATCCCGACCCAAATCGAT 70 24 0 50.00 57.196 0.00 0.00 41.60 6.804 101 GTGGGCCATTCGATCCCGACCCAA 64 24 0 62.50 62.829 8.58 0.00 43.79 6.829 102 CCCGACCCAAATCGATAGCTACGATG 78 26 0 53.85 59.158 5.23 0.00 43.02 6.842 103 GGCCATTCGATCCCGACCCAAATCG 67 25 0 60.00 61.896 0.00 0.00 41.60 6.896 104 CGATCCCGACCCAAATCGATAGCTA 74 25 0 52.00 58.095 0.00 0.00 41.28 6.905 105 GTATTAGTGGGCCATTCGATCCCGAC 58 26 0 53.85 59.095 3.57 3.02 41.60 6.905 106 ACACACACACACAGCACACAGGTATT 37 26 0 46.15 59.083 0.00 0.00 0.00 6.917 107 CAGCACACAGGTATTAGTGGGCCATT 48 26 0 50.00 59.075 8.63 0.00 32.68 6.925 108 AGACATGACACTGACGATGCAGAC 10 24 0 50.00 57.044 1.44 0.00 0.00 6.956 109 TCGATCCCGACCCAAATCGATAGCTA 73 26 0 50.00 59.025 0.30 0.00 41.28 6.975 110 CCCGACCCAAATCGATAGCTACGATGA 78 27 0 51.85 60.016 7.53 0.00 43.02 7.016 111 TCCCGACCCAAATCGATAGCTACGATG 77 27 0 51.85 60.016 5.23 0.00 43.02 7.016 112 TTAGTGGGCCATTCGATCCCGACCC 61 25 0 60.00 62.051 5.61 2.93 41.60 7.051 113 GATCCCGACCCAAATCGATAGCTACG 75 26 0 53.85 58.944 5.17 5.17 41.28 7.056 114 CGATCCCGACCCAAATCGATAGCTAC 74 26 0 53.85 58.944 0.00 0.00 41.28 7.056 115 CACACACACACAGCACACAGGTATT 38 25 0 48.00 57.933 0.00 0.00 0.00 7.067 116 AGGTATTAGTGGGCCATTCGATCCC 56 25 0 52.00 57.930 4.31 0.37 0.00 7.070 117 GCACACAGGTATTAGTGGGCCATTC 50 25 0 52.00 57.921 2.67 0.00 28.81 7.079 118 ACAGGTATTAGTGGGCCATTCGATCCC 54 27 0 51.85 59.906 4.31 0.37 0.00 7.094 119 ATTCGATCCCGACCCAAATCGATAGC 71 26 0 50.00 58.876 0.00 0.00 41.60 7.124 120 CACACACACACACAGCACACAGGTATT 36 27 0 48.15 59.861 0.00 0.00 0.00 7.139 121 GCCATTCGATCCCGACCCAAATCGAT 68 26 0 53.85 61.145 0.00 0.00 41.60 7.145 122 AGCACACAGGTATTAGTGGGCCATTC 49 26 0 50.00 58.855 8.63 0.00 32.68 7.145 123 ACAGCACACAGGTATTAGTGGGCCATT 47 27 0 48.15 60.172 8.63 0.00 32.68 7.172 124 GATCCCGACCCAAATCGATAGCTACGA 75 27 0 51.85 59.807 12.68 12.68 41.28 7.193 125 TCGATCCCGACCCAAATCGATAGCTAC 73 27 0 51.85 59.807 0.30 0.00 41.28 7.193 126 CAGGTATTAGTGGGCCATTCGATCCC 55 26 0 53.85 58.807 4.31 0.37 0.00 7.193 127 ACACAGGTATTAGTGGGCCATTCGA 52 25 0 48.00 57.761 0.00 0.00 0.00 7.239 128 TGGGCCATTCGATCCCGACCCAAAT 65 25 0 56.00 62.244 8.58 0.00 41.29 7.244 129 ACACACAGCACACAGGTATTAGTGGG 43 26 0 50.00 58.753 0.00 0.00 0.00 7.247 130 ATTCGATCCCGACCCAAATCGATAGCT 71 27 0 48.15 59.750 0.00 0.00 41.60 7.250 131 CACACAGGTATTAGTGGGCCATTCG 51 25 0 52.00 57.707 0.00 0.00 0.00 7.293 132 CATTCGATCCCGACCCAAATCGATAGC 70 27 0 51.85 59.657 0.00 0.00 41.60 7.343 133 CAGCACACAGGTATTAGTGGGCCATTC 48 27 0 51.85 59.649 8.63 0.00 32.68 7.351 134 CACACAGGTATTAGTGGGCCATTCGA 51 26 0 50.00 58.645 0.00 0.00 0.00 7.355 135 CCATTCGATCCCGACCCAAATCGATA 69 26 0 50.00 58.599 0.00 0.00 41.60 7.401 136 CACACAGCACACAGGTATTAGTGGG 44 25 0 52.00 57.592 0.00 0.00 0.00 7.408 137 CACACACAGCACACAGGTATTAGTGGG 42 27 0 51.85 59.548 0.00 0.00 0.00 7.452 138 CCGACCCAAATCGATAGCTACGATG 79 25 0 52.00 57.466 5.23 0.00 43.02 7.534 139 CGACCCAAATCGATAGCTACGATGACG 80 27 0 51.85 59.462 6.52 4.23 43.02 7.538 140 CCGACCCAAATCGATAGCTACGATGA 79 26 0 50.00 58.397 7.53 0.00 43.02 7.603 141 ATCCCGACCCAAATCGATAGCTACGAT 76 27 0 48.15 59.327 14.48 14.48 46.54 7.673 142 GCCATTCGATCCCGACCCAAATCGATA 68 27 0 51.85 60.693 0.00 0.00 41.60 7.693 143 GTATTAGTGGGCCATTCGATCCCGACC 58 27 0 55.56 60.693 5.61 0.00 41.60 7.693 144 GGTATTAGTGGGCCATTCGATCCCGAC 57 27 0 55.56 60.693 3.57 3.02 41.60 7.693 145 CAGGTATTAGTGGGCCATTCGATCCCG 55 27 0 55.56 60.694 4.31 0.00 37.07 7.694 146 ACACACACACACACAGCACACAGGTAT 35 27 0 48.15 60.696 0.00 0.00 0.00 7.696 147 CACAGCACACAGGTATTAGTGGGCCAT 46 27 0 51.85 60.700 8.63 0.00 32.68 7.700 148 TTCGATCCCGACCCAAATCGATAGCTA 72 27 0 48.15 59.277 0.00 0.00 41.60 7.723 149 GCACACAGGTATTAGTGGGCCATTCGA 50 27 0 51.85 60.748 2.67 0.00 28.81 7.748 150 AGCACACAGGTATTAGTGGGCCATTCG 49 27 0 51.85 60.749 8.63 0.00 32.68 7.749 151 ACAGGTATTAGTGGGCCATTCGATCC 54 26 0 50.00 58.246 2.01 1.68 0.00 7.754 152 CGATCCCGACCCAAATCGATAGCTACG 74 27 0 55.56 60.786 5.17 5.17 41.28 7.786 153 CCGACCCAAATCGATAGCTACGATGAC 79 27 0 51.85 59.198 5.43 0.00 43.02 7.802 154 AGGTATTAGTGGGCCATTCGATCCCGA 56 27 0 51.85 60.813 4.31 0.00 41.96 7.813 155 CCATTCGATCCCGACCCAAATCGATAG 69 27 0 51.85 59.187 0.00 0.00 41.60 7.813 156 ACACACAGCACACAGGTATTAGTGGGC 43 27 0 51.85 60.853 5.21 5.21 33.51 7.853 157 CACAGGTATTAGTGGGCCATTCGATCC 53 27 0 51.85 59.072 2.01 1.68 0.00 7.928 158 CAGGTATTAGTGGGCCATTCGATCC 55 25 0 52.00 57.061 2.01 1.68 0.00 7.939 159 GTGGGCCATTCGATCCCGACCCAAA 64 25 0 60.00 62.949 8.58 0.00 43.79 7.949 160 ACACACACAGCACACAGGTATTAGTGG 41 27 0 48.15 59.020 0.00 0.00 0.00 7.980 161 TAGTGGGCCATTCGATCCCGACCCA 62 25 0 60.00 62.992 8.58 6.40 42.67 7.992 162 ACACACAGCACACAGGTATTAGTGG 43 25 0 48.00 57.007 0.00 0.00 0.00 7.993 163 ATTAGTGGGCCATTCGATCCCGACCC 60 26 0 57.69 62.039 5.61 2.93 41.60 8.039 164 CACACACAGCACACAGGTATTAGTGG 42 26 0 50.00 57.915 0.00 0.00 0.00 8.085 165 ACACAGGTATTAGTGGGCCATTCGAT 52 26 0 46.15 57.901 0.00 0.00 0.00 8.099 166 TCAGTAGACATGACACTGACGATGCA 5 26 0 46.15 57.893 16.57 0.76 0.00 8.107 167 CACACAGGTATTAGTGGGCCATTCGAT 51 27 0 48.15 58.749 0.00 0.00 0.00 8.251 168 GTCAGTAGACATGACACTGACGATGCA 4 27 0 48.15 58.724 22.48 5.75 41.09 8.276 169 ACACACACACACAGCACACAGGTATTA 37 27 0 44.44 58.700 0.00 0.00 0.00 8.300 170 AGTAGACATGACACTGACGATGCAGA 7 26 0 46.15 57.669 7.76 0.00 0.00 8.331 171 CACACAGCACACAGGTATTAGTGGGCC 44 27 0 55.56 61.369 8.63 0.00 32.68 8.369 172 GTAGACATGACACTGACGATGCAGAC 8 26 0 50.00 57.624 1.44 0.00 0.00 8.376 173 GTCAGTAGACATGACACTGACGATGC 4 26 0 50.00 57.624 22.48 10.90 41.09 8.376 174 CAGTAGACATGACACTGACGATGCAG 6 26 0 50.00 57.620 12.05 0.00 0.00 8.380 175 CACACACACACAGCACACAGGTATTA 38 26 0 46.15 57.582 0.00 0.00 0.00 8.418 176 GACCCAAATCGATAGCTACGATGACG 81 26 0 50.00 57.576 1.59 0.00 43.02 8.424 177 CGACCCAAATCGATAGCTACGATGAC 80 26 0 50.00 57.576 2.38 0.00 43.02 8.424 178 CATTCGATCCCGACCCAAATCGATAG 70 26 0 50.00 57.547 0.00 0.00 41.60 8.453 179 ACACAGGTATTAGTGGGCCATTCGATC 52 27 0 48.15 58.537 0.00 0.00 0.00 8.463 180 CAGTAGACATGACACTGACGATGCAGA 6 27 0 48.15 58.514 12.05 0.00 0.00 8.486 181 TCAGTAGACATGACACTGACGATGCAG 5 27 0 48.15 58.514 16.57 0.00 0.00 8.486 182 AGTAGACATGACACTGACGATGCAGAC 7 27 0 48.15 58.513 1.44 0.00 0.00 8.487 183 AGTCAGTAGACATGACACTGACGATGC 3 27 0 48.15 58.513 25.46 14.56 43.89 8.487 184 ACACACACACAGCACACAGGTATTAGT 39 27 0 44.44 58.485 0.00 0.00 0.00 8.515 185 TATTAGTGGGCCATTCGATCCCGACCC 59 27 0 55.56 61.548 5.61 2.93 41.60 8.548 186 CACAGGTATTAGTGGGCCATTCGATC 53 26 0 50.00 57.410 0.00 0.00 0.00 8.590 187 ACACACACAGCACACAGGTATTAGTG 41 26 0 46.15 57.358 0.00 0.00 0.00 8.642 188 CACACACACAGCACACAGGTATTAGT 40 26 0 46.15 57.358 0.00 0.00 0.00 8.642 189 ACACACACACAGCACACAGGTATTAG 39 26 0 46.15 57.358 0.00 0.00 0.00 8.642 190 TGGGCCATTCGATCCCGACCCAAATC 65 26 0 57.69 62.687 8.58 0.00 41.29 8.687 191 GGCCATTCGATCCCGACCCAAATCGA 67 26 0 57.69 62.714 0.00 0.00 41.60 8.714 192 ACACAGCACACAGGTATTAGTGGGCCA 45 27 0 51.85 61.715 8.63 1.50 32.68 8.715 193 CACACACACAGCACACAGGTATTAGTG 40 27 0 48.15 58.213 0.00 0.00 0.00 8.787 194 CACACACACACAGCACACAGGTATTAG 38 27 0 48.15 58.213 0.00 0.00 0.00 8.787 195 GTGGGCCATTCGATCCCGACCCAAAT 64 26 0 57.69 62.905 8.58 0.00 43.79 8.905 196 GGCCATTCGATCCCGACCCAAATCGAT 67 27 0 55.56 62.679 0.00 0.00 41.60 9.679 primer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_2.rev0000664000077300007730000000626112231156244030043 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- lib qual- # sequence start ln N GC% Tm any_th end_th pin sim lity 0 CGTGCTTTGCGTTTTGGAAT 137 20 0 45.00 58.514 0.00 0.00 0.00 11.00 0.486 1 GAGCGTGCTTTGCGTTTTG 140 19 0 52.63 59.161 0.00 0.00 37.44 10.00 1.161 2 CGTGCTTTGCGTTTTGGAATA 137 21 0 42.86 58.344 0.00 0.00 0.00 12.00 1.656 3 GTTGGCGTTTCTATCAGGTCA 73 21 0 47.62 58.305 0.00 0.00 0.00 11.00 1.695 4 TCATACAGTGAGTTGGCGTT 84 20 0 45.00 57.168 0.00 0.00 31.80 11.00 1.832 5 TACAGTGAGTTGGCGTTTCT 81 20 0 45.00 57.097 0.00 0.00 0.00 12.00 1.903 6 ACAGTGAGTTGGCGTTTCTA 80 20 0 45.00 57.097 0.00 0.00 0.00 12.00 1.903 7 CGTGCTTTGCGTTTTGGAA 137 19 0 47.37 58.049 0.00 0.00 0.00 10.00 1.951 8 TGAGTTGGCGTTTCTATCAGG 76 21 0 47.62 58.027 0.00 0.00 0.00 11.00 1.973 9 GAGTTGGCGTTTCTATCAGGT 75 21 0 47.62 58.026 0.00 0.00 0.00 11.00 1.974 10 AGTTGGCGTTTCTATCAGGTC 74 21 0 47.62 58.026 0.00 0.00 0.00 11.00 1.974 11 TCATACAGTGAGTTGGCGTTT 84 21 0 42.86 57.880 0.00 0.00 31.80 12.00 2.120 12 GCGTGCTTTGCGTTTTGGAA 138 20 0 50.00 61.143 0.00 0.00 0.00 10.00 2.143 13 GGAGCGTGCTTTGCGTTTT 141 19 0 52.63 60.299 0.00 0.00 37.44 10.00 2.299 14 ATACAGTGAGTTGGCGTTTCT 82 21 0 42.86 57.597 0.00 0.00 0.00 12.00 2.403 15 ACAGTGAGTTGGCGTTTCTAT 80 21 0 42.86 57.597 0.00 0.00 0.00 12.00 2.403 16 CATACAGTGAGTTGGCGTTTC 83 21 0 47.62 57.544 0.00 0.00 0.00 12.00 2.456 17 GGAGCGTGCTTTGCGTTTTG 141 20 0 55.00 61.540 0.00 0.00 37.44 10.00 2.540 18 GAGCGTGCTTTGCGTTTTGG 140 20 0 55.00 61.540 0.00 0.00 37.44 10.00 2.540 19 GCGTGCTTTGCGTTTTGGA 138 19 0 52.63 60.588 0.00 0.00 0.00 10.00 2.588 20 AGCGTGCTTTGCGTTTTGG 139 19 0 52.63 60.589 0.00 0.00 37.44 10.00 2.589 21 TTGGCGTTTCTATCAGGTCAT 72 21 0 42.86 57.373 0.00 0.00 0.00 11.00 2.627 22 AGTGAGTTGGCGTTTCTATCA 78 21 0 42.86 57.322 0.00 0.00 0.00 11.00 2.678 23 ACAGTGAGTTGGCGTTTCT 80 19 0 47.37 57.219 0.00 0.00 0.00 12.00 2.781 24 AGCGTGCTTTGCGTTTTGGA 139 20 0 50.00 61.999 0.00 0.00 37.44 10.00 2.999 25 CAGTGAGTTGGCGTTTCTATC 79 21 0 47.62 57.001 0.00 0.00 0.00 12.00 2.999 26 GTGAGTTGGCGTTTCTATCAG 77 21 0 47.62 57.001 0.00 0.00 0.00 10.00 2.999 27 GCGTGCTTTGCGTTTTGGAAT 138 21 0 47.62 61.455 0.00 0.00 0.00 11.00 3.455 28 GGGAGCGTGCTTTGCGTTTT 142 20 0 55.00 62.678 0.00 0.00 37.44 10.00 3.678 29 GATCGGGAGCGTGCTTTGC 146 19 0 63.16 62.101 0.00 0.00 0.00 10.00 4.101 30 GGGAGCGTGCTTTGCGTTT 142 19 0 57.89 62.194 0.00 0.00 37.44 10.00 4.194 31 AGCGTGCTTTGCGTTTTGGAA 139 21 0 47.62 62.473 0.00 0.00 37.44 10.00 4.473 32 GAGCGTGCTTTGCGTTTTGGA 140 21 0 52.38 62.837 0.00 0.00 37.44 10.00 4.837 primer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_1.for0000664000077300007730000001160412231156244030031 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- lib qual- # sequence start ln N GC% Tm any_th end_th pin sim lity 0 TTAGTGGGCCATTCGATCCC 61 20 0 55.00 59.528 5.82 5.82 0.00 9.00 0.528 1 CCCGACCCAAATCGATAGCT 78 20 0 55.00 59.607 0.00 0.00 45.13 9.00 0.607 2 TCCCGACCCAAATCGATAGC 77 20 0 55.00 59.608 0.00 0.00 45.13 10.00 0.608 3 GACATGACACTGACGATGCA 11 20 0 50.00 58.286 0.00 0.00 0.00 12.00 0.714 4 CGATCCCGACCCAAATCGAT 74 20 0 55.00 59.966 0.00 0.00 45.13 11.00 0.966 5 CATTCGATCCCGACCCAAAT 70 20 0 50.00 58.033 0.00 0.00 45.50 11.00 0.967 6 CATGACACTGACGATGCAGA 13 20 0 50.00 58.000 10.65 0.00 0.00 12.00 1.000 7 ATGACACTGACGATGCAGAC 14 20 0 50.00 57.998 4.19 0.00 0.00 12.00 1.002 8 AGACATGACACTGACGATGC 10 20 0 50.00 57.998 0.00 0.00 0.00 11.00 1.002 9 CCATTCGATCCCGACCCAAA 69 20 0 55.00 60.107 0.00 0.00 45.50 10.00 1.107 10 CATTCGATCCCGACCCAAATC 70 21 0 52.38 59.126 0.00 0.00 45.50 12.00 1.126 11 TAGTGGGCCATTCGATCCC 62 19 0 57.89 58.858 5.82 5.82 0.00 9.00 1.142 12 TTCGATCCCGACCCAAATCG 72 20 0 55.00 60.179 0.00 0.00 45.50 11.00 1.179 13 ATTCGATCCCGACCCAAATC 71 20 0 50.00 57.744 0.00 0.00 45.50 12.00 1.256 14 CGACCCAAATCGATAGCTACG 80 21 0 52.38 58.683 8.77 8.77 45.13 10.00 1.317 15 CCCGACCCAAATCGATAGCTA 78 21 0 52.38 59.383 0.00 0.00 45.13 10.00 1.383 16 CCATTCGATCCCGACCCAA 69 19 0 57.89 59.475 0.00 0.00 45.50 9.00 1.475 17 CGATCCCGACCCAAATCGA 74 19 0 57.89 59.560 0.00 0.00 45.13 10.00 1.560 18 TCGATCCCGACCCAAATCG 73 19 0 57.89 59.560 0.00 0.00 40.30 11.00 1.560 19 ATTAGTGGGCCATTCGATCC 60 20 0 50.00 57.431 0.00 0.00 0.00 9.00 1.569 20 CCGACCCAAATCGATAGCTAC 79 21 0 52.38 58.326 0.00 0.00 45.13 11.00 1.674 21 AGACATGACACTGACGATGCA 10 21 0 47.62 59.727 0.00 0.00 0.00 12.00 1.727 22 CGATCCCGACCCAAATCGATA 74 21 0 52.38 59.729 0.00 0.00 45.13 10.00 1.729 23 CCCGACCCAAATCGATAGC 78 19 0 57.89 58.086 0.00 0.00 45.13 10.00 1.914 24 ATTAGTGGGCCATTCGATCCC 60 21 0 52.38 59.926 5.82 5.82 0.00 9.00 1.926 25 GATCCCGACCCAAATCGATAG 75 21 0 52.38 58.039 0.00 0.00 45.13 10.00 1.961 26 ATCCCGACCCAAATCGATAGC 76 21 0 52.38 59.998 0.00 0.00 45.13 10.00 1.998 27 TCGATCCCGACCCAAATCGA 73 20 0 55.00 61.039 4.17 2.87 45.13 10.00 2.039 28 TAGACATGACACTGACGATGC 9 21 0 47.62 57.849 3.11 0.00 0.00 11.00 2.151 29 CATTCGATCCCGACCCAAA 70 19 0 52.63 57.530 0.00 0.00 45.50 10.00 2.470 30 CCATTCGATCCCGACCCAAAT 69 21 0 52.38 60.477 0.00 0.00 45.50 11.00 2.477 31 TGACACTGACGATGCAGAC 15 19 0 52.63 57.503 4.19 0.00 0.00 11.00 2.497 32 ATTCGATCCCGACCCAAATCG 71 21 0 52.38 60.540 0.00 0.00 45.50 11.00 2.540 33 GACCCAAATCGATAGCTACGA 81 21 0 47.62 57.362 16.85 16.85 43.65 11.00 2.638 34 TATTAGTGGGCCATTCGATCC 59 21 0 47.62 57.304 0.00 0.00 0.00 9.00 2.696 35 TAGTGGGCCATTCGATCCCG 62 20 0 60.00 61.754 7.70 0.00 37.07 9.00 2.754 36 TTCGATCCCGACCCAAATC 72 19 0 52.63 57.227 0.00 0.00 45.50 12.00 2.773 37 CAGTAGACATGACACTGACGA 6 21 0 47.62 57.168 16.46 0.00 0.00 11.00 2.832 38 TCAGTAGACATGACACTGACG 5 21 0 47.62 57.168 20.80 6.93 0.00 10.00 2.832 39 ACATGACACTGACGATGCA 12 19 0 47.37 57.087 0.00 0.00 0.00 12.00 2.913 40 CCGACCCAAATCGATAGCT 79 19 0 52.63 57.011 0.00 0.00 45.13 9.00 2.989 41 TCCCGACCCAAATCGATAGCT 77 21 0 52.38 61.033 0.00 0.00 45.13 10.00 3.033 42 TCGATCCCGACCCAAATCGAT 73 21 0 52.38 61.368 3.83 0.00 45.13 11.00 3.368 43 TTCGATCCCGACCCAAATCGA 72 21 0 52.38 61.568 4.17 3.06 45.50 10.00 3.568 44 GCCATTCGATCCCGACCCAA 68 20 0 60.00 62.603 0.00 0.00 45.50 10.00 3.603 45 GCCATTCGATCCCGACCCA 68 19 0 63.16 62.102 0.00 0.00 45.50 10.00 4.102 46 TGGGCCATTCGATCCCGAC 65 19 0 63.16 62.102 7.70 0.00 45.50 9.00 4.102 47 GTGGGCCATTCGATCCCGA 64 19 0 63.16 62.102 5.82 0.00 43.96 9.00 4.102 48 AGTGGGCCATTCGATCCCG 63 19 0 63.16 62.107 7.70 0.00 37.07 9.00 4.107 49 TTAGTGGGCCATTCGATCCCG 61 21 0 57.14 62.250 7.70 0.00 37.07 9.00 4.250 50 GGCCATTCGATCCCGACCC 67 19 0 68.42 62.827 0.00 0.00 45.50 9.00 4.827 51 GGGCCATTCGATCCCGACC 66 19 0 68.42 62.827 4.39 0.00 45.50 9.00 4.827 primer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_3.int0000664000077300007730000004137112231156244030043 0ustar ioanaioanaACCEPTABLE INTERNAL OLIGOS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 GGGCCATTCGATCCCGACCC 66 20 0 70.00 59.273 2.18 0.00 41.60 0.727 1 GTGGGCCATTCGATCCCGACC 64 21 0 66.67 59.583 2.53 0.00 41.60 1.417 2 GGCCATTCGATCCCGACCCA 67 20 0 65.00 58.561 0.00 0.00 41.60 1.439 3 TGGGCCATTCGATCCCGACC 65 20 0 65.00 58.561 4.28 0.00 41.60 1.439 4 GGGCCATTCGATCCCGACCCA 66 21 0 66.67 60.650 2.16 0.00 41.60 1.650 5 TGGGCCATTCGATCCCGACCC 65 21 0 66.67 60.650 4.28 0.51 41.60 1.650 6 GGCCATTCGATCCCGACCCAA 67 21 0 61.90 58.904 0.00 0.00 41.60 2.096 7 AGTGGGCCATTCGATCCCGAC 63 21 0 61.90 58.640 3.57 3.02 41.60 2.360 8 AGTGGGCCATTCGATCCCGA 63 20 0 60.00 57.544 3.28 0.00 41.96 2.456 9 GTGGGCCATTCGATCCCGAC 64 20 0 65.00 57.489 0.97 0.00 41.60 2.511 10 AGTGGGCCATTCGATCCCGACC 63 22 0 63.64 60.627 5.61 0.00 41.60 2.627 11 GGCCATTCGATCCCGACCCAAA 67 22 0 59.09 59.216 0.00 0.00 41.60 2.784 12 GGGCCATTCGATCCCGACCCAA 66 22 0 63.64 60.885 2.16 0.00 41.60 2.885 13 TAGTGGGCCATTCGATCCCGACC 62 23 0 60.87 60.114 5.61 0.00 41.60 3.114 14 GTGGGCCATTCGATCCCGACCC 64 22 0 68.18 61.511 2.53 0.25 41.60 3.511 15 GGCCATTCGATCCCGACCCAAAT 67 23 0 56.52 59.315 0.00 0.00 41.60 3.685 16 TAGTGGGCCATTCGATCCCGAC 62 22 0 59.09 58.192 3.57 3.02 41.60 3.808 17 GCCATTCGATCCCGACCCAAA 68 21 0 57.14 57.174 0.00 0.00 41.60 3.826 18 ACACACACACACAGCACACAGGT 37 23 0 52.17 59.135 0.00 0.00 0.00 3.865 19 TAGTGGGCCATTCGATCCCGA 62 21 0 57.14 57.119 0.00 0.00 41.96 3.881 20 TGGGCCATTCGATCCCGACCCA 65 22 0 63.64 61.937 8.58 6.38 42.67 3.937 21 GGCCATTCGATCCCGACCC 67 19 0 68.42 57.043 0.00 0.00 41.60 3.957 22 GGGCCATTCGATCCCGACC 66 19 0 68.42 57.043 2.18 0.00 41.60 3.957 23 CACACACACACACAGCACACAGGT 36 24 0 54.17 60.001 0.00 0.00 0.00 4.001 24 ACACACACACACACAGCACACAGG 35 24 0 54.17 60.001 0.00 0.00 0.00 4.001 25 GGCCATTCGATCCCGACCCAAATC 67 24 0 58.33 59.942 0.00 0.00 41.60 4.058 26 GGGCCATTCGATCCCGACCCAAA 66 23 0 60.87 61.100 2.16 0.00 41.60 4.100 27 CACACACACACAGCACACAGGT 38 22 0 54.55 57.841 0.00 0.00 0.00 4.159 28 ACACACACACACAGCACACAGG 37 22 0 54.55 57.841 0.00 0.00 0.00 4.159 29 CACACACACACACAGCACACAGG 36 23 0 56.52 58.804 0.00 0.00 0.00 4.196 30 GCCATTCGATCCCGACCCAAATCG 68 24 0 58.33 60.233 0.00 0.00 41.60 4.233 31 TTAGTGGGCCATTCGATCCCGACC 61 24 0 58.33 60.352 5.61 0.00 41.60 4.352 32 TTAGTGGGCCATTCGATCCCGAC 61 23 0 56.52 58.521 3.57 3.02 41.60 4.479 33 TTAGTGGGCCATTCGATCCCGA 61 22 0 54.55 57.516 0.00 0.00 41.96 4.484 34 ACACACACACACACAGCACACA 35 22 0 50.00 57.448 0.00 0.00 0.00 4.552 35 GCCATTCGATCCCGACCCAAAT 68 22 0 54.55 57.361 0.00 0.00 41.60 4.639 36 ACACACACACACACAGCACACAG 35 23 0 52.17 58.191 0.00 0.00 0.00 4.809 37 GCCATTCGATCCCGACCCAAATC 68 23 0 56.52 58.119 0.00 0.00 41.60 4.881 38 TGGGCCATTCGATCCCGACCCAA 65 23 0 60.87 62.108 8.58 0.00 41.29 5.108 39 CCATTCGATCCCGACCCAAATCGA 69 24 0 54.17 58.889 0.00 0.00 41.60 5.111 40 CCATTCGATCCCGACCCAAATCG 69 23 0 56.52 57.889 0.00 0.00 41.60 5.111 41 ACAGCACACAGGTATTAGTGGGCCA 47 25 0 52.00 59.886 8.63 1.50 32.68 5.114 42 GGGCCATTCGATCCCGACCCAAAT 66 24 0 58.33 61.123 2.16 0.00 41.60 5.123 43 AGCACACAGGTATTAGTGGGCCA 49 23 0 52.17 57.732 8.63 1.50 32.68 5.268 44 ACACACACACACAGCACACAGGTA 37 24 0 50.00 58.705 0.00 0.00 0.00 5.295 45 CAGCACACAGGTATTAGTGGGCCA 48 24 0 54.17 58.694 8.63 1.50 32.68 5.306 46 ACAGCACACAGGTATTAGTGGGCC 47 24 0 54.17 58.693 8.63 0.00 32.68 5.307 47 ATTAGTGGGCCATTCGATCCCGA 60 23 0 52.17 57.684 0.00 0.00 41.96 5.316 48 ATTAGTGGGCCATTCGATCCCGAC 60 24 0 54.17 58.642 3.57 3.02 41.60 5.358 49 ATTAGTGGGCCATTCGATCCCGACC 60 25 0 56.00 60.401 5.61 0.00 41.60 5.401 50 CACACACACACACAGCACACAGGTA 36 25 0 52.00 59.560 0.00 0.00 0.00 5.440 51 CACAGCACACAGGTATTAGTGGGCC 46 25 0 56.00 59.556 8.63 0.00 32.68 5.444 52 AGTGGGCCATTCGATCCCGACCC 63 23 0 65.22 62.459 5.61 2.93 41.60 5.459 53 TCGATCCCGACCCAAATCGATAGC 73 24 0 54.17 58.485 0.30 0.00 41.28 5.515 54 CGATCCCGACCCAAATCGATAGCT 74 24 0 54.17 58.481 0.00 0.00 41.28 5.519 55 CGATCCCGACCCAAATCGATAGC 74 23 0 56.52 57.476 0.00 0.00 41.28 5.524 56 CACACACACACAGCACACAGGTA 38 23 0 52.17 57.450 0.00 0.00 0.00 5.550 57 TCGATCCCGACCCAAATCGATAGCT 73 25 0 52.00 59.436 0.30 0.00 41.28 5.564 58 CAGCACACAGGTATTAGTGGGCC 48 23 0 56.52 57.427 8.63 0.00 32.68 5.573 59 GTGGGCCATTCGATCCCGACCCA 64 23 0 65.22 62.698 8.58 6.40 42.67 5.698 60 CCCGACCCAAATCGATAGCTACGA 78 24 0 54.17 58.193 12.68 12.68 41.28 5.807 61 TCCCGACCCAAATCGATAGCTACG 77 24 0 54.17 58.193 5.17 5.17 41.28 5.807 62 CCCGACCCAAATCGATAGCTACG 78 23 0 56.52 57.176 5.17 5.17 41.28 5.824 63 TCCCGACCCAAATCGATAGCTACGA 77 25 0 52.00 59.154 12.68 12.68 41.28 5.846 64 TAGTGGGCCATTCGATCCCGACCC 62 24 0 62.50 61.893 5.61 2.93 41.60 5.893 65 GGTATTAGTGGGCCATTCGATCCCG 57 25 0 56.00 59.049 4.28 0.00 37.07 5.951 66 CATTCGATCCCGACCCAAATCGA 70 23 0 52.17 57.019 0.00 0.00 41.60 5.981 67 CCATTCGATCCCGACCCAAATCGAT 69 25 0 52.00 58.991 0.00 0.00 41.60 6.009 68 ACACAGCACACAGGTATTAGTGGGC 45 25 0 52.00 58.985 5.21 5.21 33.51 6.015 69 TATTAGTGGGCCATTCGATCCCGACC 59 26 0 53.85 59.954 5.61 0.00 41.60 6.046 70 GGTATTAGTGGGCCATTCGATCCCGA 57 26 0 53.85 59.954 0.76 0.00 41.96 6.046 71 AGGTATTAGTGGGCCATTCGATCCCG 56 26 0 53.85 59.953 4.31 0.00 37.07 6.047 72 ACAGCACACAGGTATTAGTGGGCCAT 47 26 0 50.00 59.951 8.63 0.00 32.68 6.049 73 GCACACAGGTATTAGTGGGCCATTCG 50 26 0 53.85 59.904 2.67 0.00 28.81 6.096 74 AGCACACAGGTATTAGTGGGCCAT 49 24 0 50.00 57.886 8.63 0.00 32.68 6.114 75 GCCATTCGATCCCGACCCAAATCGA 68 25 0 56.00 61.125 0.00 0.00 41.60 6.125 76 ACACACACACACACAGCACACAGGT 35 25 0 52.00 61.132 0.00 0.00 0.00 6.132 77 ACACACACACACAGCACACAGGTAT 37 25 0 48.00 58.814 0.00 0.00 0.00 6.186 78 CAGCACACAGGTATTAGTGGGCCAT 48 25 0 52.00 58.804 8.63 0.00 32.68 6.196 79 CACACAGCACACAGGTATTAGTGGGC 44 26 0 53.85 59.796 5.21 5.21 33.51 6.204 80 CACAGCACACAGGTATTAGTGGGC 46 24 0 54.17 57.786 5.21 5.21 33.51 6.214 81 TTCGATCCCGACCCAAATCGATAGC 72 25 0 52.00 58.774 0.00 0.00 41.60 6.226 82 TGGGCCATTCGATCCCGACCCAAA 65 24 0 58.33 62.265 8.58 0.00 41.29 6.265 83 TTCGATCCCGACCCAAATCGATAGCT 72 26 0 50.00 59.681 0.00 0.00 41.60 6.319 84 CACACACACACACAGCACACAGGTAT 36 26 0 50.00 59.634 0.00 0.00 0.00 6.366 85 CACACACACACAGCACACAGGTAT 38 24 0 50.00 57.610 0.00 0.00 0.00 6.390 86 GGGCCATTCGATCCCGACCCAAATC 66 25 0 60.00 61.636 2.16 0.00 41.60 6.636 87 ACACACACACACACAGCACACAGGTA 35 26 0 50.00 60.661 0.00 0.00 0.00 6.661 88 CACAGCACACAGGTATTAGTGGGCCA 46 26 0 53.85 60.665 8.63 1.50 32.68 6.665 89 ACACAGCACACAGGTATTAGTGGGCC 45 26 0 53.85 60.665 8.63 0.00 32.68 6.665 90 CCCGACCCAAATCGATAGCTACGAT 78 25 0 52.00 58.320 14.48 14.48 46.54 6.680 91 ATCCCGACCCAAATCGATAGCTACG 76 25 0 52.00 58.320 5.17 5.17 41.28 6.680 92 TATTAGTGGGCCATTCGATCCCGA 59 24 0 50.00 57.304 0.00 0.00 41.96 6.696 93 GTATTAGTGGGCCATTCGATCCCG 58 24 0 54.17 57.258 4.28 0.00 37.07 6.742 94 TATTAGTGGGCCATTCGATCCCGAC 59 25 0 52.00 58.246 3.57 3.02 41.60 6.754 95 GTATTAGTGGGCCATTCGATCCCGA 58 25 0 52.00 58.246 0.00 0.00 41.96 6.754 96 TCCCGACCCAAATCGATAGCTACGAT 77 26 0 50.00 59.244 14.48 14.48 46.54 6.756 97 ATCCCGACCCAAATCGATAGCTACGA 76 26 0 50.00 59.244 12.68 12.68 41.28 6.756 98 AGCACACAGGTATTAGTGGGCCATT 49 25 0 48.00 58.208 8.63 0.00 32.68 6.792 99 GCACACAGGTATTAGTGGGCCATT 50 24 0 50.00 57.207 2.67 0.00 28.81 6.793 100 CATTCGATCCCGACCCAAATCGAT 70 24 0 50.00 57.196 0.00 0.00 41.60 6.804 101 GTGGGCCATTCGATCCCGACCCAA 64 24 0 62.50 62.829 8.58 0.00 43.79 6.829 102 CCCGACCCAAATCGATAGCTACGATG 78 26 0 53.85 59.158 5.23 0.00 43.02 6.842 103 GGCCATTCGATCCCGACCCAAATCG 67 25 0 60.00 61.896 0.00 0.00 41.60 6.896 104 CGATCCCGACCCAAATCGATAGCTA 74 25 0 52.00 58.095 0.00 0.00 41.28 6.905 105 GTATTAGTGGGCCATTCGATCCCGAC 58 26 0 53.85 59.095 3.57 3.02 41.60 6.905 106 ACACACACACACAGCACACAGGTATT 37 26 0 46.15 59.083 0.00 0.00 0.00 6.917 107 CAGCACACAGGTATTAGTGGGCCATT 48 26 0 50.00 59.075 8.63 0.00 32.68 6.925 108 AGACATGACACTGACGATGCAGAC 10 24 0 50.00 57.044 1.44 0.00 0.00 6.956 109 TCGATCCCGACCCAAATCGATAGCTA 73 26 0 50.00 59.025 0.30 0.00 41.28 6.975 110 CCCGACCCAAATCGATAGCTACGATGA 78 27 0 51.85 60.016 7.53 0.00 43.02 7.016 111 TCCCGACCCAAATCGATAGCTACGATG 77 27 0 51.85 60.016 5.23 0.00 43.02 7.016 112 TTAGTGGGCCATTCGATCCCGACCC 61 25 0 60.00 62.051 5.61 2.93 41.60 7.051 113 GATCCCGACCCAAATCGATAGCTACG 75 26 0 53.85 58.944 5.17 5.17 41.28 7.056 114 CGATCCCGACCCAAATCGATAGCTAC 74 26 0 53.85 58.944 0.00 0.00 41.28 7.056 115 CACACACACACAGCACACAGGTATT 38 25 0 48.00 57.933 0.00 0.00 0.00 7.067 116 AGGTATTAGTGGGCCATTCGATCCC 56 25 0 52.00 57.930 4.31 0.37 0.00 7.070 117 GCACACAGGTATTAGTGGGCCATTC 50 25 0 52.00 57.921 2.67 0.00 28.81 7.079 118 ACAGGTATTAGTGGGCCATTCGATCCC 54 27 0 51.85 59.906 4.31 0.37 0.00 7.094 119 ATTCGATCCCGACCCAAATCGATAGC 71 26 0 50.00 58.876 0.00 0.00 41.60 7.124 120 CACACACACACACAGCACACAGGTATT 36 27 0 48.15 59.861 0.00 0.00 0.00 7.139 121 GCCATTCGATCCCGACCCAAATCGAT 68 26 0 53.85 61.145 0.00 0.00 41.60 7.145 122 AGCACACAGGTATTAGTGGGCCATTC 49 26 0 50.00 58.855 8.63 0.00 32.68 7.145 123 ACAGCACACAGGTATTAGTGGGCCATT 47 27 0 48.15 60.172 8.63 0.00 32.68 7.172 124 GATCCCGACCCAAATCGATAGCTACGA 75 27 0 51.85 59.807 12.68 12.68 41.28 7.193 125 TCGATCCCGACCCAAATCGATAGCTAC 73 27 0 51.85 59.807 0.30 0.00 41.28 7.193 126 CAGGTATTAGTGGGCCATTCGATCCC 55 26 0 53.85 58.807 4.31 0.37 0.00 7.193 127 ACACAGGTATTAGTGGGCCATTCGA 52 25 0 48.00 57.761 0.00 0.00 0.00 7.239 128 TGGGCCATTCGATCCCGACCCAAAT 65 25 0 56.00 62.244 8.58 0.00 41.29 7.244 129 ACACACAGCACACAGGTATTAGTGGG 43 26 0 50.00 58.753 0.00 0.00 0.00 7.247 130 ATTCGATCCCGACCCAAATCGATAGCT 71 27 0 48.15 59.750 0.00 0.00 41.60 7.250 131 CACACAGGTATTAGTGGGCCATTCG 51 25 0 52.00 57.707 0.00 0.00 0.00 7.293 132 CATTCGATCCCGACCCAAATCGATAGC 70 27 0 51.85 59.657 0.00 0.00 41.60 7.343 133 CAGCACACAGGTATTAGTGGGCCATTC 48 27 0 51.85 59.649 8.63 0.00 32.68 7.351 134 CACACAGGTATTAGTGGGCCATTCGA 51 26 0 50.00 58.645 0.00 0.00 0.00 7.355 135 CCATTCGATCCCGACCCAAATCGATA 69 26 0 50.00 58.599 0.00 0.00 41.60 7.401 136 CACACAGCACACAGGTATTAGTGGG 44 25 0 52.00 57.592 0.00 0.00 0.00 7.408 137 CACACACAGCACACAGGTATTAGTGGG 42 27 0 51.85 59.548 0.00 0.00 0.00 7.452 138 CCGACCCAAATCGATAGCTACGATG 79 25 0 52.00 57.466 5.23 0.00 43.02 7.534 139 CGACCCAAATCGATAGCTACGATGACG 80 27 0 51.85 59.462 6.52 4.23 43.02 7.538 140 CCGACCCAAATCGATAGCTACGATGA 79 26 0 50.00 58.397 7.53 0.00 43.02 7.603 141 ATCCCGACCCAAATCGATAGCTACGAT 76 27 0 48.15 59.327 14.48 14.48 46.54 7.673 142 GCCATTCGATCCCGACCCAAATCGATA 68 27 0 51.85 60.693 0.00 0.00 41.60 7.693 143 GTATTAGTGGGCCATTCGATCCCGACC 58 27 0 55.56 60.693 5.61 0.00 41.60 7.693 144 GGTATTAGTGGGCCATTCGATCCCGAC 57 27 0 55.56 60.693 3.57 3.02 41.60 7.693 145 CAGGTATTAGTGGGCCATTCGATCCCG 55 27 0 55.56 60.694 4.31 0.00 37.07 7.694 146 ACACACACACACACAGCACACAGGTAT 35 27 0 48.15 60.696 0.00 0.00 0.00 7.696 147 CACAGCACACAGGTATTAGTGGGCCAT 46 27 0 51.85 60.700 8.63 0.00 32.68 7.700 148 TTCGATCCCGACCCAAATCGATAGCTA 72 27 0 48.15 59.277 0.00 0.00 41.60 7.723 149 GCACACAGGTATTAGTGGGCCATTCGA 50 27 0 51.85 60.748 2.67 0.00 28.81 7.748 150 AGCACACAGGTATTAGTGGGCCATTCG 49 27 0 51.85 60.749 8.63 0.00 32.68 7.749 151 ACAGGTATTAGTGGGCCATTCGATCC 54 26 0 50.00 58.246 2.01 1.68 0.00 7.754 152 CGATCCCGACCCAAATCGATAGCTACG 74 27 0 55.56 60.786 5.17 5.17 41.28 7.786 153 CCGACCCAAATCGATAGCTACGATGAC 79 27 0 51.85 59.198 5.43 0.00 43.02 7.802 154 AGGTATTAGTGGGCCATTCGATCCCGA 56 27 0 51.85 60.813 4.31 0.00 41.96 7.813 155 CCATTCGATCCCGACCCAAATCGATAG 69 27 0 51.85 59.187 0.00 0.00 41.60 7.813 156 ACACACAGCACACAGGTATTAGTGGGC 43 27 0 51.85 60.853 5.21 5.21 33.51 7.853 157 CACAGGTATTAGTGGGCCATTCGATCC 53 27 0 51.85 59.072 2.01 1.68 0.00 7.928 158 CAGGTATTAGTGGGCCATTCGATCC 55 25 0 52.00 57.061 2.01 1.68 0.00 7.939 159 GTGGGCCATTCGATCCCGACCCAAA 64 25 0 60.00 62.949 8.58 0.00 43.79 7.949 160 ACACACACAGCACACAGGTATTAGTGG 41 27 0 48.15 59.020 0.00 0.00 0.00 7.980 161 TAGTGGGCCATTCGATCCCGACCCA 62 25 0 60.00 62.992 8.58 6.40 42.67 7.992 162 ACACACAGCACACAGGTATTAGTGG 43 25 0 48.00 57.007 0.00 0.00 0.00 7.993 163 ATTAGTGGGCCATTCGATCCCGACCC 60 26 0 57.69 62.039 5.61 2.93 41.60 8.039 164 CACACACAGCACACAGGTATTAGTGG 42 26 0 50.00 57.915 0.00 0.00 0.00 8.085 165 ACACAGGTATTAGTGGGCCATTCGAT 52 26 0 46.15 57.901 0.00 0.00 0.00 8.099 166 TCAGTAGACATGACACTGACGATGCA 5 26 0 46.15 57.893 16.57 0.76 0.00 8.107 167 CACACAGGTATTAGTGGGCCATTCGAT 51 27 0 48.15 58.749 0.00 0.00 0.00 8.251 168 GTCAGTAGACATGACACTGACGATGCA 4 27 0 48.15 58.724 22.48 5.75 41.09 8.276 169 ACACACACACACAGCACACAGGTATTA 37 27 0 44.44 58.700 0.00 0.00 0.00 8.300 170 AGTAGACATGACACTGACGATGCAGA 7 26 0 46.15 57.669 7.76 0.00 0.00 8.331 171 CACACAGCACACAGGTATTAGTGGGCC 44 27 0 55.56 61.369 8.63 0.00 32.68 8.369 172 GTAGACATGACACTGACGATGCAGAC 8 26 0 50.00 57.624 1.44 0.00 0.00 8.376 173 GTCAGTAGACATGACACTGACGATGC 4 26 0 50.00 57.624 22.48 10.90 41.09 8.376 174 CAGTAGACATGACACTGACGATGCAG 6 26 0 50.00 57.620 12.05 0.00 0.00 8.380 175 CACACACACACAGCACACAGGTATTA 38 26 0 46.15 57.582 0.00 0.00 0.00 8.418 176 GACCCAAATCGATAGCTACGATGACG 81 26 0 50.00 57.576 1.59 0.00 43.02 8.424 177 CGACCCAAATCGATAGCTACGATGAC 80 26 0 50.00 57.576 2.38 0.00 43.02 8.424 178 CATTCGATCCCGACCCAAATCGATAG 70 26 0 50.00 57.547 0.00 0.00 41.60 8.453 179 ACACAGGTATTAGTGGGCCATTCGATC 52 27 0 48.15 58.537 0.00 0.00 0.00 8.463 180 CAGTAGACATGACACTGACGATGCAGA 6 27 0 48.15 58.514 12.05 0.00 0.00 8.486 181 TCAGTAGACATGACACTGACGATGCAG 5 27 0 48.15 58.514 16.57 0.00 0.00 8.486 182 AGTAGACATGACACTGACGATGCAGAC 7 27 0 48.15 58.513 1.44 0.00 0.00 8.487 183 AGTCAGTAGACATGACACTGACGATGC 3 27 0 48.15 58.513 25.46 14.56 43.89 8.487 184 ACACACACACAGCACACAGGTATTAGT 39 27 0 44.44 58.485 0.00 0.00 0.00 8.515 185 TATTAGTGGGCCATTCGATCCCGACCC 59 27 0 55.56 61.548 5.61 2.93 41.60 8.548 186 CACAGGTATTAGTGGGCCATTCGATC 53 26 0 50.00 57.410 0.00 0.00 0.00 8.590 187 ACACACACAGCACACAGGTATTAGTG 41 26 0 46.15 57.358 0.00 0.00 0.00 8.642 188 CACACACACAGCACACAGGTATTAGT 40 26 0 46.15 57.358 0.00 0.00 0.00 8.642 189 ACACACACACAGCACACAGGTATTAG 39 26 0 46.15 57.358 0.00 0.00 0.00 8.642 190 TGGGCCATTCGATCCCGACCCAAATC 65 26 0 57.69 62.687 8.58 0.00 41.29 8.687 191 GGCCATTCGATCCCGACCCAAATCGA 67 26 0 57.69 62.714 0.00 0.00 41.60 8.714 192 ACACAGCACACAGGTATTAGTGGGCCA 45 27 0 51.85 61.715 8.63 1.50 32.68 8.715 193 CACACACACAGCACACAGGTATTAGTG 40 27 0 48.15 58.213 0.00 0.00 0.00 8.787 194 CACACACACACAGCACACAGGTATTAG 38 27 0 48.15 58.213 0.00 0.00 0.00 8.787 195 GTGGGCCATTCGATCCCGACCCAAAT 64 26 0 57.69 62.905 8.58 0.00 43.79 8.905 196 GGCCATTCGATCCCGACCCAAATCGAT 67 27 0 55.56 62.679 0.00 0.00 41.60 9.679 primer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_3.rev0000664000077300007730000000655112231156244030046 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- lib qual- # sequence start ln N GC% Tm any_th end_th pin sim lity 0 GGGATCGAATGGCCCACTAA 80 20 0 55.00 59.528 10.16 0.00 32.36 10.00 0.528 1 AGCTATCGATTTGGGTCGGG 97 20 0 55.00 59.607 0.00 0.00 41.74 11.00 0.607 2 GCTATCGATTTGGGTCGGGA 96 20 0 55.00 59.608 1.64 0.00 41.74 12.00 0.608 3 TGCATCGTCAGTGTCATGTC 30 20 0 50.00 58.286 0.00 0.00 0.00 12.00 0.714 4 ATCGATTTGGGTCGGGATCG 93 20 0 55.00 59.966 0.00 0.00 41.74 12.00 0.966 5 GTCTGCATCGTCAGTGTCAT 33 20 0 50.00 57.998 0.00 0.00 0.00 12.00 1.002 6 GCATCGTCAGTGTCATGTCT 29 20 0 50.00 57.998 0.00 0.00 0.00 11.00 1.002 7 GGGATCGAATGGCCCACTA 80 19 0 57.89 58.858 10.16 0.00 32.36 10.00 1.142 8 CGTAGCTATCGATTTGGGTCG 100 21 0 52.38 58.683 9.28 4.23 42.74 11.00 1.317 9 TAGCTATCGATTTGGGTCGGG 98 21 0 52.38 59.383 0.00 0.00 41.74 11.00 1.383 10 TCGATTTGGGTCGGGATCG 92 19 0 57.89 59.560 0.00 0.00 41.74 12.00 1.560 11 CGATTTGGGTCGGGATCGA 91 19 0 57.89 59.560 0.00 0.00 43.86 12.00 1.560 12 GGATCGAATGGCCCACTAAT 79 20 0 50.00 57.431 0.00 0.00 0.00 11.00 1.569 13 GTAGCTATCGATTTGGGTCGG 99 21 0 52.38 58.326 0.00 0.00 41.74 11.00 1.674 14 TGCATCGTCAGTGTCATGTCT 30 21 0 47.62 59.727 1.94 0.00 0.00 12.00 1.727 15 TATCGATTTGGGTCGGGATCG 94 21 0 52.38 59.729 1.95 0.00 41.74 12.00 1.729 16 GCTATCGATTTGGGTCGGG 96 19 0 57.89 58.086 1.64 0.00 41.74 11.00 1.914 17 GGGATCGAATGGCCCACTAAT 80 21 0 52.38 59.926 10.16 0.00 32.36 11.00 1.926 18 CTATCGATTTGGGTCGGGATC 95 21 0 52.38 58.039 0.00 0.00 41.74 12.00 1.961 19 GCTATCGATTTGGGTCGGGAT 96 21 0 52.38 59.998 1.64 0.00 41.74 12.00 1.998 20 TCGATTTGGGTCGGGATCGA 92 20 0 55.00 61.039 5.62 4.35 43.86 12.00 2.039 21 GCATCGTCAGTGTCATGTCTA 29 21 0 47.62 57.849 0.00 0.00 0.00 11.00 2.151 22 GTCTGCATCGTCAGTGTCA 33 19 0 52.63 57.503 0.00 0.00 0.00 12.00 2.497 23 TCGTAGCTATCGATTTGGGTC 101 21 0 47.62 57.362 14.37 0.00 0.00 11.00 2.638 24 GGATCGAATGGCCCACTAATA 79 21 0 47.62 57.304 0.00 0.00 0.00 11.00 2.696 25 CGGGATCGAATGGCCCACTA 81 20 0 60.00 61.754 14.03 0.00 39.00 10.00 2.754 26 TCGTCAGTGTCATGTCTACTG 26 21 0 47.62 57.168 15.66 15.66 0.00 11.00 2.832 27 CGTCAGTGTCATGTCTACTGA 25 21 0 47.62 57.168 19.25 17.68 0.00 11.00 2.832 28 TGCATCGTCAGTGTCATGT 30 19 0 47.37 57.087 3.35 0.00 0.00 12.00 2.913 29 AGCTATCGATTTGGGTCGG 97 19 0 52.63 57.011 0.00 0.00 41.74 10.00 2.989 30 AGCTATCGATTTGGGTCGGGA 97 21 0 52.38 61.033 0.00 0.00 41.74 12.00 3.033 31 ATCGATTTGGGTCGGGATCGA 93 21 0 52.38 61.368 7.64 7.64 43.86 12.00 3.368 32 TCGGGATCGAATGGCCCAC 82 19 0 63.16 62.102 14.03 0.00 43.03 10.00 4.102 33 CGGGATCGAATGGCCCACT 81 19 0 63.16 62.107 14.03 0.00 39.00 10.00 4.107 34 CGGGATCGAATGGCCCACTAA 81 21 0 57.14 62.250 14.03 0.00 39.00 10.00 4.250 primer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_4.rev0000664000077300007730000000626112231156244030045 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- lib qual- # sequence start ln N GC% Tm any_th end_th pin sim lity 0 CGTGCTTTGCGTTTTGGAAT 137 20 0 45.00 58.514 0.00 0.00 0.00 11.00 0.486 1 GAGCGTGCTTTGCGTTTTG 140 19 0 52.63 59.161 0.00 0.00 37.44 10.00 1.161 2 CGTGCTTTGCGTTTTGGAATA 137 21 0 42.86 58.344 0.00 0.00 0.00 12.00 1.656 3 GTTGGCGTTTCTATCAGGTCA 73 21 0 47.62 58.305 0.00 0.00 0.00 11.00 1.695 4 TCATACAGTGAGTTGGCGTT 84 20 0 45.00 57.168 0.00 0.00 31.80 11.00 1.832 5 TACAGTGAGTTGGCGTTTCT 81 20 0 45.00 57.097 0.00 0.00 0.00 12.00 1.903 6 ACAGTGAGTTGGCGTTTCTA 80 20 0 45.00 57.097 0.00 0.00 0.00 12.00 1.903 7 CGTGCTTTGCGTTTTGGAA 137 19 0 47.37 58.049 0.00 0.00 0.00 10.00 1.951 8 TGAGTTGGCGTTTCTATCAGG 76 21 0 47.62 58.027 0.00 0.00 0.00 11.00 1.973 9 GAGTTGGCGTTTCTATCAGGT 75 21 0 47.62 58.026 0.00 0.00 0.00 11.00 1.974 10 AGTTGGCGTTTCTATCAGGTC 74 21 0 47.62 58.026 0.00 0.00 0.00 11.00 1.974 11 TCATACAGTGAGTTGGCGTTT 84 21 0 42.86 57.880 0.00 0.00 31.80 12.00 2.120 12 GCGTGCTTTGCGTTTTGGAA 138 20 0 50.00 61.143 0.00 0.00 0.00 10.00 2.143 13 GGAGCGTGCTTTGCGTTTT 141 19 0 52.63 60.299 0.00 0.00 37.44 10.00 2.299 14 ATACAGTGAGTTGGCGTTTCT 82 21 0 42.86 57.597 0.00 0.00 0.00 12.00 2.403 15 ACAGTGAGTTGGCGTTTCTAT 80 21 0 42.86 57.597 0.00 0.00 0.00 12.00 2.403 16 CATACAGTGAGTTGGCGTTTC 83 21 0 47.62 57.544 0.00 0.00 0.00 12.00 2.456 17 GGAGCGTGCTTTGCGTTTTG 141 20 0 55.00 61.540 0.00 0.00 37.44 10.00 2.540 18 GAGCGTGCTTTGCGTTTTGG 140 20 0 55.00 61.540 0.00 0.00 37.44 10.00 2.540 19 GCGTGCTTTGCGTTTTGGA 138 19 0 52.63 60.588 0.00 0.00 0.00 10.00 2.588 20 AGCGTGCTTTGCGTTTTGG 139 19 0 52.63 60.589 0.00 0.00 37.44 10.00 2.589 21 TTGGCGTTTCTATCAGGTCAT 72 21 0 42.86 57.373 0.00 0.00 0.00 11.00 2.627 22 AGTGAGTTGGCGTTTCTATCA 78 21 0 42.86 57.322 0.00 0.00 0.00 11.00 2.678 23 ACAGTGAGTTGGCGTTTCT 80 19 0 47.37 57.219 0.00 0.00 0.00 12.00 2.781 24 AGCGTGCTTTGCGTTTTGGA 139 20 0 50.00 61.999 0.00 0.00 37.44 10.00 2.999 25 CAGTGAGTTGGCGTTTCTATC 79 21 0 47.62 57.001 0.00 0.00 0.00 12.00 2.999 26 GTGAGTTGGCGTTTCTATCAG 77 21 0 47.62 57.001 0.00 0.00 0.00 10.00 2.999 27 GCGTGCTTTGCGTTTTGGAAT 138 21 0 47.62 61.455 0.00 0.00 0.00 11.00 3.455 28 GGGAGCGTGCTTTGCGTTTT 142 20 0 55.00 62.678 0.00 0.00 37.44 10.00 3.678 29 GATCGGGAGCGTGCTTTGC 146 19 0 63.16 62.101 0.00 0.00 0.00 10.00 4.101 30 GGGAGCGTGCTTTGCGTTT 142 19 0 57.89 62.194 0.00 0.00 37.44 10.00 4.194 31 AGCGTGCTTTGCGTTTTGGAA 139 21 0 47.62 62.473 0.00 0.00 37.44 10.00 4.473 32 GAGCGTGCTTTGCGTTTTGGA 140 21 0 52.38 62.837 0.00 0.00 37.44 10.00 4.837 primer3-2.3.6/test/th-w-other-tasks_list_last/pick_primer_list_and_file_flag_th_2.int0000664000077300007730000001452112231156244030037 0ustar ioanaioanaACCEPTABLE INTERNAL OLIGOS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 AACGCAAAGCACGCTCCCGA 125 20 0 60.00 59.906 7.82 0.00 36.42 0.094 1 ACGCAAAGCACGCTCCCGAT 126 20 0 60.00 59.699 7.82 0.00 36.42 0.301 2 AAACGCAAAGCACGCTCCCG 124 20 0 60.00 59.100 1.50 1.50 41.14 0.900 3 CGCAAAGCACGCTCCCGATC 127 20 0 65.00 59.043 0.00 0.00 36.42 0.957 4 AACGCAAAGCACGCTCCCGAT 125 21 0 57.14 59.982 7.82 0.00 36.42 1.018 5 AAACGCAAAGCACGCTCCCGA 124 21 0 57.14 60.179 7.82 0.00 36.42 1.179 6 ACGCAAAGCACGCTCCCGATC 126 21 0 61.90 60.373 7.82 0.00 36.42 1.373 7 ACGCAAAGCACGCTCCCGA 126 19 0 63.16 59.606 7.82 0.00 36.42 1.394 8 AAAACGCAAAGCACGCTCCCG 123 21 0 57.14 59.405 1.50 1.50 41.14 1.595 9 CCAAAACGCAAAGCACGCTCCC 121 22 0 59.09 59.992 0.00 0.00 0.00 2.008 10 AACGCAAAGCACGCTCCCG 125 19 0 63.16 58.764 1.50 1.50 41.14 2.236 11 AAACGCAAAGCACGCTCCCGAT 124 22 0 54.55 60.240 7.82 0.00 36.42 2.240 12 CAAAACGCAAAGCACGCTCCCG 122 22 0 59.09 60.299 1.50 1.50 41.14 2.299 13 AAAACGCAAAGCACGCTCCCGA 123 22 0 54.55 60.427 7.82 0.00 36.42 2.427 14 AACGCAAAGCACGCTCCCGATC 125 22 0 59.09 60.611 7.82 0.00 36.42 2.611 15 CGCAAAGCACGCTCCCGAT 127 19 0 63.16 58.260 0.00 0.00 36.42 2.740 16 TCCAAAACGCAAAGCACGCTCC 120 22 0 54.55 59.120 0.00 0.00 0.00 2.880 17 CAAAACGCAAAGCACGCTCCC 122 21 0 57.14 58.061 0.00 0.00 0.00 2.939 18 CCAAAACGCAAAGCACGCTCC 121 21 0 57.14 58.061 0.00 0.00 0.00 2.939 19 AAAACGCAAAGCACGCTCCCGAT 123 23 0 52.17 60.477 7.82 0.00 36.42 3.477 20 TTCCAAAACGCAAAGCACGCTCC 119 23 0 52.17 59.401 0.00 0.00 0.00 3.599 21 ACGCAAAGCACGCTCCCG 126 18 0 66.67 58.394 1.50 1.50 41.14 3.606 22 AAACGCAAAGCACGCTCCCGATC 124 23 0 56.52 60.829 7.82 0.00 36.42 3.829 23 TCCAAAACGCAAAGCACGCTC 120 21 0 52.38 57.135 0.00 0.00 0.00 3.865 24 CGCAAAGCACGCTCCCGA 127 18 0 66.67 58.095 0.00 0.00 36.42 3.905 25 TCCAAAACGCAAAGCACGCTCCC 120 23 0 56.52 60.955 0.00 0.00 0.00 3.955 26 CAAAACGCAAAGCACGCTCCCGA 122 23 0 56.52 61.240 7.82 0.00 36.42 4.240 27 TTCCAAAACGCAAAGCACGCTC 119 22 0 50.00 57.513 0.00 0.00 0.00 4.487 28 ATTCCAAAACGCAAAGCACGCTCC 118 24 0 50.00 59.486 0.00 0.00 0.00 4.514 29 AAAACGCAAAGCACGCTCCCGATC 123 24 0 54.17 61.030 0.00 0.00 36.42 5.030 30 CCAAAACGCAAAGCACGCTCCCG 121 23 0 60.87 62.052 1.50 1.50 41.14 5.052 31 TTCCAAAACGCAAAGCACGCTCCC 119 24 0 54.17 61.152 0.00 0.00 0.00 5.152 32 CAAAACGCAAAGCACGCTCCCGAT 122 24 0 54.17 61.257 7.82 0.00 36.42 5.257 33 ATTCCAAAACGCAAAGCACGCTC 118 23 0 47.83 57.674 0.00 0.00 0.00 5.326 34 TATTCCAAAACGCAAAGCACGCTCC 117 25 0 48.00 59.068 0.00 0.00 0.00 5.932 35 ATTCCAAAACGCAAAGCACGCTCCC 118 25 0 52.00 61.171 0.00 0.00 0.00 6.171 36 CTATTCCAAAACGCAAAGCACGCTCC 116 26 0 50.00 59.646 0.00 0.00 0.00 6.354 37 TATTCCAAAACGCAAAGCACGCTC 117 24 0 45.83 57.311 0.00 0.00 0.00 6.689 38 TCTATTCCAAAACGCAAAGCACGC 115 24 0 45.83 57.311 0.00 0.00 0.00 6.689 39 CTATTCCAAAACGCAAAGCACGCT 116 24 0 45.83 57.305 0.00 0.00 0.00 6.695 40 TATTCCAAAACGCAAAGCACGCTCCC 117 26 0 50.00 60.707 0.00 0.00 0.00 6.707 41 TCTATTCCAAAACGCAAAGCACGCT 115 25 0 44.00 58.276 0.00 0.00 0.00 6.724 42 CAAAACGCAAAGCACGCTCCCGATC 122 25 0 56.00 61.744 0.00 0.00 36.42 6.744 43 GTCTATTCCAAAACGCAAAGCACGC 114 25 0 48.00 58.214 0.00 0.00 0.00 6.786 44 GTCTATTCCAAAACGCAAAGCACGCT 114 26 0 46.15 59.111 0.00 0.00 0.00 6.889 45 AGTCTATTCCAAAACGCAAAGCACGC 113 26 0 46.15 59.111 0.00 0.00 0.00 6.889 46 CCAAAACGCAAAGCACGCTCCCGA 121 24 0 58.33 62.910 7.82 0.00 36.42 6.910 47 TCCAAAACGCAAAGCACGCTCCCG 120 24 0 58.33 62.910 1.50 1.50 41.14 6.910 48 CTATTCCAAAACGCAAAGCACGCTC 116 25 0 48.00 57.992 0.00 0.00 0.00 7.008 49 AGTCTATTCCAAAACGCAAAGCACGCT 113 27 0 44.44 59.965 0.00 0.00 0.00 7.035 50 TCTATTCCAAAACGCAAAGCACGCTC 115 26 0 46.15 58.898 0.00 0.00 0.00 7.102 51 GTCTATTCCAAAACGCAAAGCACGCTC 114 27 0 48.15 59.666 0.00 0.00 0.00 7.334 52 TCTATTCCAAAACGCAAAGCACGCTCC 115 27 0 48.15 60.481 0.00 0.00 0.00 7.481 53 AAGTCTATTCCAAAACGCAAAGCACGC 112 27 0 44.44 59.353 0.00 0.00 0.00 7.647 54 ACCTGATAGAAACGCCAACTCACTGT 55 26 0 46.15 58.256 0.00 0.00 0.00 7.744 55 CCAAAACGCAAAGCACGCTCCCGAT 121 25 0 56.00 62.867 7.82 0.00 36.42 7.867 56 TGACCTGATAGAAACGCCAACTCAC 53 25 0 48.00 57.083 0.00 0.00 0.00 7.917 57 CCTGATAGAAACGCCAACTCACTGT 56 25 0 48.00 57.077 0.00 0.00 0.00 7.923 58 ACCTGATAGAAACGCCAACTCACTG 55 25 0 48.00 57.077 0.00 0.00 0.00 7.923 59 TGACCTGATAGAAACGCCAACTCACT 53 26 0 46.15 58.036 0.00 0.00 0.00 7.964 60 TGACCTGATAGAAACGCCAACTCACTG 53 27 0 48.15 58.868 0.00 0.00 0.00 8.132 61 GACCTGATAGAAACGCCAACTCACTGT 54 27 0 48.15 58.868 0.00 0.00 0.00 8.132 62 CTATTCCAAAACGCAAAGCACGCTCCC 116 27 0 51.85 61.193 0.00 0.00 0.00 8.193 63 GACCTGATAGAAACGCCAACTCACTG 54 26 0 50.00 57.762 0.00 0.00 0.00 8.238 64 ATGACCTGATAGAAACGCCAACTCAC 52 26 0 46.15 57.245 0.00 0.00 0.00 8.755 65 AAGTCTATTCCAAAACGCAAAGCACG 112 26 0 42.31 57.241 0.00 0.00 0.00 8.759 66 ATGACCTGATAGAAACGCCAACTCACT 52 27 0 44.44 58.161 0.00 0.00 0.00 8.839 67 ATTCCAAAACGCAAAGCACGCTCCCG 118 26 0 53.85 62.978 1.50 1.50 41.14 8.978 68 ACCTGATAGAAACGCCAACTCACTGTA 55 27 0 44.44 57.900 0.00 0.00 0.00 9.100 69 AAAGTCTATTCCAAAACGCAAAGCACG 111 27 0 40.74 57.551 0.00 0.00 0.00 9.449 70 TATTCCAAAACGCAAAGCACGCTCCCG 117 27 0 51.85 62.472 1.50 1.50 41.14 9.472 71 CGTTGTCAAAGTCTATTCCAAAACGCA 104 27 0 40.74 57.087 0.00 0.00 0.00 9.913 72 ACGTTGTCAAAGTCTATTCCAAAACGC 103 27 0 40.74 57.086 0.00 0.00 0.00 9.914 primer3-2.3.6/test/primer_thermod_align_formatted_output0000775000077300007730000016050312231156245022647 0ustar ioanaioanaPRIMER PICKING RESULTS FOR thermod_default No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 11 20 59.70 55.00 0.00 0.00 0.00 GACTTCACTTTCCGACCCTG RIGHT PRIMER 113 20 59.96 50.00 9.59 0.00 0.00 TGACGGTCATCATGGTGAGT INTERNAL OLIGO 44 20 60.71 60.00 0.00 0.00 40.53 CACCCACGAGTGAAGACTCC SEQUENCE SIZE: 178 INCLUDED REGION SIZE: 178 PRODUCT SIZE: 103, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGA >>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^ 60 CTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGA ^^^^ <<<<<<<<<<<<<<<<<<<< 120 GGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 10 20 59.70 50.00 0.00 0.00 0.00 TGACTTCACTTTCCGACCCT RIGHT PRIMER 113 20 59.96 50.00 9.59 0.00 0.00 TGACGGTCATCATGGTGAGT INTERNAL OLIGO 44 20 60.71 60.00 0.00 0.00 40.53 CACCCACGAGTGAAGACTCC PRODUCT SIZE: 104, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 11 20 59.70 55.00 0.00 0.00 0.00 GACTTCACTTTCCGACCCTG RIGHT PRIMER 114 20 60.11 55.00 9.19 0.00 0.00 CTGACGGTCATCATGGTGAG INTERNAL OLIGO 44 20 60.71 60.00 0.00 0.00 40.53 CACCCACGAGTGAAGACTCC PRODUCT SIZE: 104, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 10 20 59.70 50.00 0.00 0.00 0.00 TGACTTCACTTTCCGACCCT RIGHT PRIMER 114 20 60.11 55.00 9.19 0.00 0.00 CTGACGGTCATCATGGTGAG INTERNAL OLIGO 44 20 60.71 60.00 0.00 0.00 40.53 CACCCACGAGTGAAGACTCC PRODUCT SIZE: 105, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 2 20 59.29 50.00 0.00 0.00 0.00 GCCGAGGATGACTTCACTTT RIGHT PRIMER 113 20 59.96 50.00 9.59 0.00 0.00 TGACGGTCATCATGGTGAGT INTERNAL OLIGO 44 20 60.71 60.00 0.00 0.00 40.53 CACCCACGAGTGAAGACTCC PRODUCT SIZE: 112, PAIR ANY_TH COMPL: 8.76, PAIR 3'_TH COMPL: 2.04 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 1290 0 0 0 0 10 0 60 1039 0 0 0 0 0 181 Right 1290 0 0 0 0 32 0 38 1096 0 0 0 0 0 124 Intl 1565 0 0 0 0 32 0 60 1290 0 0 0 0 0 183 Pair Stats: considered 25, unacceptable product size 20, ok 5 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR any_1 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 17 20 60.01 50.00 2.42 0.00 42.83 CAAGCAATACGACCTGCTGA RIGHT PRIMER 168 20 59.98 50.00 0.00 0.00 39.48 ATCGCAAGTGAGGATGAGCT INTERNAL OLIGO 116 21 60.58 38.10 12.25 10.49 37.82 CGTGTGCAAAACATTTTCACA SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC >>>>>>>>>>>>>>>>>>>> 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG ^^^^ 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^ <<<<<<<<<<<<<<<<<<<< 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 17 20 60.01 50.00 2.42 0.00 42.83 CAAGCAATACGACCTGCTGA RIGHT PRIMER 191 20 59.94 40.00 5.65 0.00 39.80 CAAAAGATTTTGGCGGGTAA INTERNAL OLIGO 149 20 59.98 50.00 0.00 0.00 0.00 AGCTCATCCTCACTTGCGAT PRODUCT SIZE: 175, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 17 20 60.01 50.00 2.42 0.00 42.83 CAAGCAATACGACCTGCTGA RIGHT PRIMER 368 20 59.75 60.00 8.58 0.00 0.00 CCGTACGCACTATAGGAGCC INTERNAL OLIGO 149 20 59.98 50.00 0.00 0.00 0.00 AGCTCATCCTCACTTGCGAT PRODUCT SIZE: 352, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 149 20 59.98 50.00 0.00 0.00 0.00 AGCTCATCCTCACTTGCGAT RIGHT PRIMER 368 20 59.75 60.00 8.58 0.00 0.00 CCGTACGCACTATAGGAGCC INTERNAL OLIGO 172 20 59.94 40.00 4.21 4.21 43.62 TTACCCGCCAAAATCTTTTG PRODUCT SIZE: 220, PAIR ANY_TH COMPL: 7.44, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 17 20 60.01 50.00 2.42 0.00 42.83 CAAGCAATACGACCTGCTGA RIGHT PRIMER 232 20 59.70 50.00 0.00 0.00 0.00 CACTAAAGCGGGATTTGAGG INTERNAL OLIGO 149 20 59.98 50.00 0.00 0.00 0.00 AGCTCATCCTCACTTGCGAT PRODUCT SIZE: 216, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3397 0 0 0 0 0 0 897 1543 0 0 0 22 0 935 Right 3397 0 0 0 0 0 0 839 1673 0 0 1 22 0 862 Intl 3672 0 0 0 0 0 0 953 1734 0 0 14 22 0 949 Pair Stats: considered 58, unacceptable product size 51, ok 7 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR any_salt No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 103 19 59.90 42.11 2.59 0.51 0.00 TCTATCTGTACAACGTGTG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3397 0 0 0 0 0 0 52 3139 0 0 1 22 0 183 Right 3397 0 0 0 0 0 0 59 3137 0 0 0 22 0 179 Intl 3672 0 0 0 0 0 0 432 2545 0 0 10 22 0 663 Pair Stats: considered 15, unacceptable product size 10, ok 5 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR any_weight No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 103 19 59.90 42.11 2.59 0.51 0.00 TCTATCTGTACAACGTGTG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3397 0 0 0 0 0 0 52 3139 0 0 1 22 0 183 Right 3397 0 0 0 0 0 0 59 3137 0 0 0 22 0 179 Intl 3672 0 0 0 0 0 0 432 2545 0 0 10 22 0 663 Pair Stats: considered 15, unacceptable product size 10, ok 5 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR any_2 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 103 19 59.90 42.11 2.59 0.51 0.00 TCTATCTGTACAACGTGTG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3155 0 0 0 0 0 0 52 2924 32 0 1 22 0 124 Right 3213 0 0 0 0 0 0 59 2968 23 0 0 19 0 144 Intl 3185 0 0 0 0 0 0 432 2188 82 0 10 22 0 451 Pair Stats: considered 15, unacceptable product size 10, ok 5 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR any_no_effect No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3397 0 0 0 0 0 0 52 3139 1 0 1 22 0 182 Right 3397 0 0 0 0 0 0 59 3137 0 0 0 22 0 179 Intl 3672 0 0 0 0 0 0 432 2545 11 0 4 22 0 658 Pair Stats: considered 17, unacceptable product size 11, ok 6 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR end_th1 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3397 0 0 0 0 0 0 52 3139 1 0 1 22 0 182 Right 3397 0 0 0 0 0 0 59 3137 0 0 0 22 0 179 Intl 3672 0 0 0 0 0 0 432 2545 11 0 4 22 0 658 Pair Stats: considered 17, unacceptable product size 11, ok 6 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR end_th2 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3075 0 0 0 0 0 0 52 2860 42 0 0 22 0 99 Right 3118 0 0 0 0 0 0 59 2890 38 0 0 17 0 114 Intl 2916 0 0 0 0 0 0 432 1999 116 0 4 22 0 343 Pair Stats: considered 14, unacceptable product size 5, ok 9 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR hairpin1 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3075 0 0 0 0 0 0 52 2860 42 0 0 22 0 99 Right 3118 0 0 0 0 0 0 59 2890 38 0 0 17 0 114 Intl 2916 0 0 0 0 0 0 432 1999 116 0 4 22 0 343 Pair Stats: considered 14, unacceptable product size 5, ok 9 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR hairpin2 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3075 0 0 0 0 0 0 52 2860 42 0 0 22 0 99 Right 3118 0 0 0 0 0 0 59 2890 38 0 0 17 0 114 Intl 2916 0 0 0 0 0 0 432 1999 116 0 4 22 0 343 Pair Stats: considered 14, unacceptable product size 5, ok 9 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR hairpin3 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3075 0 0 0 0 0 0 52 2860 42 0 0 22 0 99 Right 3118 0 0 0 0 0 0 59 2890 38 0 0 17 0 114 Intl 2916 0 0 0 0 0 0 432 1999 116 0 22 22 0 325 Pair Stats: considered 14, unacceptable product size 5, ok 9 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR template1 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3075 0 0 0 0 0 0 52 2860 42 0 0 22 0 99 Right 3118 0 0 0 0 0 0 59 2890 38 0 0 17 0 114 Intl 2916 0 0 0 0 0 0 432 1999 116 0 22 22 0 325 Pair Stats: considered 14, unacceptable product size 5, ok 9 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR template2 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- poly end ered Ns get reg reg GC% clamp low high compl compl pin X stab ok Left 3075 0 0 0 0 0 0 52 2860 42 0 0 22 0 99 Right 3118 0 0 0 0 0 0 59 2890 38 0 0 17 0 114 Intl 2916 0 0 0 0 0 0 432 1999 116 0 22 22 0 325 Pair Stats: considered 14, unacceptable product size 5, ok 9 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR template2_and_repeat_library Using mispriming library ../src/humrep_and_simple.txt No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin rep seq LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 12.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 11.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG >>>>>>>>>>>>>>>>>>>> 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC ^^^^^^^^^^^^^^^^^^^^ 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<<<<<<<<<<<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin rep seq 1 LEFT PRIMER 98 20 60.84 30.00 0.00 0.00 0.00 12.00 ATTTTTCTATCTGTACAACG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 12.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 11.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 254 20 59.66 45.00 0.00 0.00 0.00 11.00 GACCTGTAATAGCTCACTAC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 224 20 60.39 45.00 0.00 0.00 0.00 11.00 GCTTTAGTGTAGTAGTGAGC RIGHT PRIMER 401 20 61.03 40.00 0.00 0.00 0.00 12.00 ATACTGATGTCTATAGTGCG INTERNAL OLIGO 340 20 59.83 45.00 0.00 0.00 0.00 GCAGAAAAAGGCTCCTATAG PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 99 20 61.09 30.00 0.00 0.00 0.00 11.00 TTTTTCTATCTGTACAACGT RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 12.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 145 20 59.87 45.00 0.00 0.00 0.00 AAACAGCTCATCCTCACTTG PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- lib poly end ered Ns get reg reg GC% clamp low high compl compl pin sim X stab ok Left 3075 0 0 0 0 0 0 52 2860 42 0 0 0 22 0 99 Right 3118 0 0 0 0 0 0 59 2890 38 0 0 0 17 0 114 Intl 2916 0 0 0 0 0 0 432 1999 116 0 22 0 22 0 325 Pair Stats: considered 14, unacceptable product size 5, ok 9 libprimer3 release 2.3.5 PRIMER PICKING RESULTS FOR template3 Using mispriming library ../src/humrep_and_simple.txt No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC OLIGO start len tm gc% any_th 3'_th hairpin rep seq LEFT PRIMER 8 19 59.84 31.58 0.00 0.00 0.00 12.00 CATTCAATACAAGCAATAC RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 12.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 72 20 59.96 45.00 0.00 0.00 0.00 GCTCATTAAGACCGTACGAT SEQUENCE SIZE: 404 INCLUDED REGION SIZE: 404 PRODUCT SIZE: 236, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC >>>>>>>>>>>>>>>>>>> 60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG ^^^^^^^^^^^^^^^^^^^^ 120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC 180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT <<<<<<<<<<<<<<<< 240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC <<<< 300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG 360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any_th 3'_th hairpin rep seq 1 LEFT PRIMER 46 19 60.82 31.58 0.00 0.00 0.00 10.00 ATATTCAGTTCATCAAAGG RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 12.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 72 20 59.96 45.00 0.00 0.00 0.00 GCTCATTAAGACCGTACGAT PRODUCT SIZE: 198, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 2 LEFT PRIMER 8 19 59.84 31.58 0.00 0.00 0.00 12.00 CATTCAATACAAGCAATAC RIGHT PRIMER 241 19 59.75 42.11 0.00 0.00 0.00 11.00 TCACTACTACACTAAAGCG INTERNAL OLIGO 72 20 59.96 45.00 0.00 0.00 0.00 GCTCATTAAGACCGTACGAT PRODUCT SIZE: 234, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 3 LEFT PRIMER 7 20 62.06 30.00 0.00 0.00 0.00 12.00 TCATTCAATACAAGCAATAC RIGHT PRIMER 243 20 60.39 45.00 0.00 0.00 0.00 12.00 GCTCACTACTACACTAAAGC INTERNAL OLIGO 72 20 59.96 45.00 0.00 0.00 0.00 GCTCATTAAGACCGTACGAT PRODUCT SIZE: 237, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 4 LEFT PRIMER 8 19 59.84 31.58 0.00 0.00 0.00 12.00 CATTCAATACAAGCAATAC RIGHT PRIMER 242 20 61.41 45.00 0.00 0.00 0.00 12.00 CTCACTACTACACTAAAGCG INTERNAL OLIGO 72 20 59.96 45.00 0.00 0.00 0.00 GCTCATTAAGACCGTACGAT PRODUCT SIZE: 235, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00 Statistics con too in in not no tm tm high high high high high sid many tar excl ok bad GC too too any_th 3'_th hair- lib poly end ered Ns get reg reg GC% clamp low high compl compl pin sim X stab ok Left 3075 0 0 0 0 0 0 52 2860 42 0 18 3 22 0 78 Right 3118 0 0 0 0 0 0 59 2890 38 0 25 0 17 0 89 Intl 2916 0 0 0 0 0 0 432 1999 116 0 22 0 22 0 325 Pair Stats: considered 7017, unacceptable product size 2861, high any compl 150, no internal oligo 210, high template mispriming score 97, ok 3699 libprimer3 release 2.3.5 primer3-2.3.6/test/primer_quality_boundary_output0000664000077300007730000004212412231156245021354 0ustar ioanaioanaSEQUENCE_ID=quality_test1 PRIMER_EXPLAIN_FLAG=1 P3_COMMENT=quality scores out of range PRIMER_MIN_TM=0 PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 PRIMER_MAX_TM=100 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=20 PRIMER_PAIR_MAX_COMPL_ANY=20 PRIMER_PAIR_MAX_COMPL_END=20 PRIMER_PRODUCT_SIZE_RANGE=50-70 70-90 90-110 110-500 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC PRIMER_MIN_QUALITY=30 PRIMER_QUALITY_RANGE_MAX=40 SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_ERROR=Sequence quality score out of range = SEQUENCE_ID=quality_test2 P3_COMMENT=quality scores out of range SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_MIN_QUALITY=60 PRIMER_QUALITY_RANGE_MIN=60 PRIMER_QUALITY_RANGE_MAX=65 PRIMER_ERROR=Sequence quality score out of range = SEQUENCE_ID=quality_test3 P3_COMMENT=quality scores out of range SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_MIN_QUALITY=9 PRIMER_QUALITY_RANGE_MAX=49 PRIMER_ERROR=Sequence quality score out of range = SEQUENCE_ID=quality_test4 P3_COMMENT=Should return exactly one pair SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC PRIMER_MIN_QUALITY=60 PRIMER_QUALITY_RANGE_MAX=70 PRIMER_MIN_SIZE=10 SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 PRIMER_LEFT_EXPLAIN=considered 89, low sequence quality 88, ok 1 PRIMER_RIGHT_EXPLAIN=considered 89, low sequence quality 88, ok 1 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=104.232823 PRIMER_LEFT_0_PENALTY=52.299978 PRIMER_RIGHT_0_PENALTY=51.932846 PRIMER_LEFT_0_SEQUENCE=ATCGTAGCTG PRIMER_RIGHT_0_SEQUENCE=GCATCGACTA PRIMER_LEFT_0=0,10 PRIMER_RIGHT_0=120,10 PRIMER_LEFT_0_TM=17.700 PRIMER_RIGHT_0_TM=18.067 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_MIN_SEQ_QUALITY=60 PRIMER_RIGHT_0_MIN_SEQ_QUALITY=60 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=5.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=121 = SEQUENCE_ID=quality_test5 P3_COMMENT=Should return exactly one pair PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 PRIMER_LEFT_EXPLAIN=considered 89, low sequence quality 88, ok 1 PRIMER_RIGHT_EXPLAIN=considered 89, low sequence quality 88, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 111, low sequence quality 110, ok 1 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=104.232823 PRIMER_LEFT_0_PENALTY=52.299978 PRIMER_RIGHT_0_PENALTY=51.932846 PRIMER_INTERNAL_0_PENALTY=44.970813 PRIMER_LEFT_0_SEQUENCE=ATCGTAGCTG PRIMER_RIGHT_0_SEQUENCE=GCATCGACTA PRIMER_INTERNAL_0_SEQUENCE=CATCATCGTACT PRIMER_LEFT_0=0,10 PRIMER_RIGHT_0=120,10 PRIMER_INTERNAL_0=73,12 PRIMER_LEFT_0_TM=17.700 PRIMER_RIGHT_0_TM=18.067 PRIMER_INTERNAL_0_TM=23.029 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_MIN_SEQ_QUALITY=60 PRIMER_RIGHT_0_MIN_SEQ_QUALITY=60 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=5.4000 PRIMER_INTERNAL_0_MIN_SEQ_QUALITY=55 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=121 = SEQUENCE_ID=quality_test6 P3_COMMENT=Should fail because of low quality for middle oligo PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=51 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 53 54 54 54 54 50 54 54 54 54 54 51 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 PRIMER_LEFT_EXPLAIN=considered 89, low sequence quality 88, ok 1 PRIMER_RIGHT_EXPLAIN=considered 89, low sequence quality 88, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 110, low sequence quality 110, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=quality_test7 P3_COMMENT=Should fail as there are too few score values PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=20 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_ERROR=Error in sequence quality data = SEQUENCE_ID=quality_test8 P3_COMMENT=Should fail as there are too many score values PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=20 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY= 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_ERROR=Error in sequence quality data = SEQUENCE_ID=quality_test9 P3_COMMENT=Should fail because of low end quality PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 PRIMER_LEFT_EXPLAIN=considered 89, low sequence quality 89, ok 0 PRIMER_RIGHT_EXPLAIN=considered 89, low sequence quality 89, ok 0 PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=quality_test10 P3_COMMENT=Should return exactly one pair PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 60 60 60 60 60 PRIMER_LEFT_EXPLAIN=considered 89, low sequence quality 88, ok 1 PRIMER_RIGHT_EXPLAIN=considered 89, low sequence quality 88, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 111, low sequence quality 110, ok 1 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=104.232823 PRIMER_LEFT_0_PENALTY=52.299978 PRIMER_RIGHT_0_PENALTY=51.932846 PRIMER_INTERNAL_0_PENALTY=44.970813 PRIMER_LEFT_0_SEQUENCE=ATCGTAGCTG PRIMER_RIGHT_0_SEQUENCE=GCATCGACTA PRIMER_INTERNAL_0_SEQUENCE=CATCATCGTACT PRIMER_LEFT_0=0,10 PRIMER_RIGHT_0=120,10 PRIMER_INTERNAL_0=73,12 PRIMER_LEFT_0_TM=17.700 PRIMER_RIGHT_0_TM=18.067 PRIMER_INTERNAL_0_TM=23.029 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_MIN_SEQ_QUALITY=60 PRIMER_RIGHT_0_MIN_SEQ_QUALITY=60 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=5.4000 PRIMER_INTERNAL_0_MIN_SEQ_QUALITY=55 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=121 = SEQUENCE_ID=quality_test11 P3_COMMENT=Should fail as high quality pairs are not in the included region PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_INCLUDED_REGION=2,115 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 60 60 60 60 60 PRIMER_LEFT_EXPLAIN=considered 83, low sequence quality 82, ok 1 PRIMER_RIGHT_EXPLAIN=considered 83, low sequence quality 83, ok 0 PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=quality_test12 P3_COMMENT=Should return exactly one pair PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_INCLUDED_REGION=2,115 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 61 61 61 61 60 50 50 50 50 PRIMER_LEFT_EXPLAIN=considered 83, low sequence quality 82, ok 1 PRIMER_RIGHT_EXPLAIN=considered 83, low sequence quality 82, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 105, low sequence quality 104, ok 1 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=108.457122 PRIMER_LEFT_0_PENALTY=52.299978 PRIMER_RIGHT_0_PENALTY=56.157144 PRIMER_INTERNAL_0_PENALTY=44.970813 PRIMER_LEFT_0_SEQUENCE=CGTAGCTGAT PRIMER_RIGHT_0_SEQUENCE=CGACTAGCTA PRIMER_INTERNAL_0_SEQUENCE=CATCATCGTACT PRIMER_LEFT_0=2,10 PRIMER_RIGHT_0=116,10 PRIMER_INTERNAL_0=73,12 PRIMER_LEFT_0_TM=17.700 PRIMER_RIGHT_0_TM=13.843 PRIMER_INTERNAL_0_TM=23.029 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_MIN_SEQ_QUALITY=60 PRIMER_RIGHT_0_MIN_SEQ_QUALITY=60 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_INTERNAL_0_MIN_SEQ_QUALITY=55 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=115 = SEQUENCE_ID=quality_test13 P3_COMMENT=Should return exactly one pair PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_INCLUDED_REGION=2,115 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60.33 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 61 61 61 61 60 50 50 50 50 PRIMER_ERROR=Error in sequence quality data = SEQUENCE_ID=quality_test14 P3_COMMENT=Should return exactly one pair PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_INCLUDED_REGION=2,115 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY= 60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 61 61 61 61 60 50 50 50 50 PRIMER_LEFT_EXPLAIN=considered 83, low sequence quality 82, ok 1 PRIMER_RIGHT_EXPLAIN=considered 83, low sequence quality 82, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 105, low sequence quality 104, ok 1 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=108.457122 PRIMER_LEFT_0_PENALTY=52.299978 PRIMER_RIGHT_0_PENALTY=56.157144 PRIMER_INTERNAL_0_PENALTY=44.970813 PRIMER_LEFT_0_SEQUENCE=CGTAGCTGAT PRIMER_RIGHT_0_SEQUENCE=CGACTAGCTA PRIMER_INTERNAL_0_SEQUENCE=CATCATCGTACT PRIMER_LEFT_0=2,10 PRIMER_RIGHT_0=116,10 PRIMER_INTERNAL_0=73,12 PRIMER_LEFT_0_TM=17.700 PRIMER_RIGHT_0_TM=13.843 PRIMER_INTERNAL_0_TM=23.029 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_MIN_SEQ_QUALITY=60 PRIMER_RIGHT_0_MIN_SEQ_QUALITY=60 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_INTERNAL_0_MIN_SEQ_QUALITY=55 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=115 = primer3-2.3.6/test/primer_ok_regions2_output0000664000077300007730000270136112231156245020211 0ustar ioanaioanaSEQUENCE_ID=ok_regions_test1 SEQUENCE_TEMPLATE=GGATCAGCTGACTCGCCTGGCTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGCCGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTGTGTGGCAGCGCCATGTCCCAAAGTTTACTCAAGACTACCCAGGAACCTCTTGCCCGAGATCCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGATGAGCTGCTTCAGAAAGAGCAAAACTATTCAGATGACGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCATCTTTGACCGAAACGAAAACCACCGTGGAGCTCCTTCCCGTGAATGGAGAGTTCAGCCTGGACGATCTCCAGCCGTGGCATTCTTTTGGGGCTGACTCTGTGCCAGCCAACACAGAAAACGAAGTTGAGCCTGTTGATGCCCGCCCTGCTGCCGACCGAGGACTGACCACTCGACCAGGTTCTGGGTTGACAAATATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGACTCAGGATATGAAGTTCATCATCAAAAATTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGTGCAATCATTGGACTCATGGTGGGCGGTGTTGTCATAGCGACAGTGATCGTCATCACCTTGGTGATGCTGAAGAAGAAACAGTACACATCCATTCATCATGGTGTGGTGGAGGTTGACGCCGCTGTCACCCCAGAGGAGCGCCACCTGTCCAAGATGCAGCAGAACGGCTACGAAAATCCAACCTACAAGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCTCTGAAGTTGGACAGCAAAACCATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGGGAAGAAACAAACCCGTTTTATGATTTACTCATTATCGCCTTTTGACAGCTGTGCTGTAACACAAGTAGATGCCTGAACTTGAATTAATCCACACATCAGTAATGTATTCTATCTCTCTTTACATTTTGGTCTCTATACTACATTATTAATGGGTTTTGTGTACTGTAAAGAATTTAGCTGTATCAAACTAGTGCATGAATAGATTCTCTCCTGATTATTTATCACATAGCCCCTTAGCCAGTTGTATATTATTCTTGTGGTTTGTGACCCAATTAAGTCCTACTTTACATATGCTTTAAGAATCGATGGGGGATGCTTCATGTGAACGTGGGAGTTCAGCTGCTTCTCTTGCCTAAGTATTCCTTTCCTGATCACTATGCATTTTAAAGTTAAACATTTTTAAGTATTTCAGATGCTTTAGAGAGATTTTTTTTCCATGACTGCATTTTACTGTACAGATTGCTGCTTCTGCTATATTTGTGATATAGGAATTAAGAGGATACACACGTTTGTTTCTTCGTGCCTGTTTTATGTGCACACATTAGGCATTGAGACTTCAAGCTTTTCTTTTTTTGTCCACGTATCTTTGGGTCTTTGATAAAGAAAAGAATCCCTGTTCATTGTAAGCACTTTTACGGGGCGGGTGGGGAGGGGTGCTCTGCTGGTCTTCAATTACCAAGAATTCTCCAAAACAATTTTCTGCAGGATGATTGTACAGAATCATTGCTTATGACATGATCGCTTTCTACACTGTATTACATAAATAAATTAAATAAAATAACCCCGGGCAAGACTTTTCTTTGAAGGATGACTACAGACATTAAATAATCGAAGTAATTTTGGGTGGGGAGAAGAGGCAGATTCAATTTTCTTTAACCAGTCTGAAGTTTCATTTATGATACAAAAGAAGATGAAAATGGAAGTGGCAATATAAGGGGATGAGGAAGGCATGCCTGGACAAACCCTTCTTTTAAGATGTGTCTTCAATTTGTATAAAATGGTGTTTTCATGTAAATAAATACATTCTTGGAGGAGCAAAAAAAAAAAAAAAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-1000 PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_THREE_PRIME_DISTANCE=0 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,216,,;,,238,216 SEQUENCE_OVERLAP_JUNCTION_LIST=256 424 554 668 861 1064 1232 1289 1423 1498 1657 1786 1886 2108 2162 2263 2410 PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION=4 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=7 PRIMER_LEFT_EXPLAIN=considered 34490, GC content failed 759, low tm 295, high tm 2741, high any compl 17, high end compl 93, not in any ok left region 30165, ok 420 PRIMER_RIGHT_EXPLAIN=considered 34490, GC content failed 15, low tm 2036, high tm 6898, high any compl 4, high end compl 47, not in any ok right region 22715, ok 2775 PRIMER_PAIR_EXPLAIN=considered 839536, unacceptable product size 21069, high end compl 38, no overlap of required point 818172, primer in pair overlaps a primer in a better pair 264217, ok 257 PRIMER_LEFT_NUM_RETURNED=200 PRIMER_RIGHT_NUM_RETURNED=200 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=200 PRIMER_PAIR_0_PENALTY=0.120586 PRIMER_LEFT_0_PENALTY=0.112124 PRIMER_RIGHT_0_PENALTY=0.008461 PRIMER_LEFT_0_SEQUENCE=CAGATTGCCATGTTCTGTGG PRIMER_RIGHT_0_SEQUENCE=TCACTGCATGTCTCTTTGGC PRIMER_LEFT_0=296,20 PRIMER_RIGHT_0=678,20 PRIMER_LEFT_0_TM=60.112 PRIMER_RIGHT_0_TM=59.992 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=10.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=383 PRIMER_PAIR_1_PENALTY=0.290503 PRIMER_LEFT_1_PENALTY=0.112124 PRIMER_RIGHT_1_PENALTY=0.178378 PRIMER_LEFT_1_SEQUENCE=CCAGATTGCCATGTTCTGTG PRIMER_RIGHT_1_SEQUENCE=TGTTCAGAGCACACCTCTCG PRIMER_LEFT_1=295,20 PRIMER_RIGHT_1=1080,20 PRIMER_LEFT_1_TM=60.112 PRIMER_RIGHT_1_TM=60.178 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=786 PRIMER_PAIR_2_PENALTY=0.502773 PRIMER_LEFT_2_PENALTY=0.137602 PRIMER_RIGHT_2_PENALTY=0.365170 PRIMER_LEFT_2_SEQUENCE=GGAAGTGGGATTCAGATCCA PRIMER_RIGHT_2_SEQUENCE=GTAAACTTTGGGACATGGCG PRIMER_LEFT_2=348,20 PRIMER_RIGHT_2=1247,20 PRIMER_LEFT_2_TM=59.862 PRIMER_RIGHT_2_TM=60.365 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=11.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=900 PRIMER_PAIR_3_PENALTY=0.554615 PRIMER_LEFT_3_PENALTY=0.532607 PRIMER_RIGHT_3_PENALTY=0.022009 PRIMER_LEFT_3_SEQUENCE=GCTGGAGGTACCCACTGATG PRIMER_RIGHT_3_SEQUENCE=TGCTGGTGGTTCTCTCTGTG PRIMER_LEFT_3=250,20 PRIMER_RIGHT_3=1013,20 PRIMER_LEFT_3_TM=60.533 PRIMER_RIGHT_3_TM=60.022 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=764 PRIMER_PAIR_4_PENALTY=0.673790 PRIMER_LEFT_4_PENALTY=0.220845 PRIMER_RIGHT_4_PENALTY=0.452945 PRIMER_LEFT_4_SEQUENCE=ATGTCCAGAATGGGAAGTGG PRIMER_RIGHT_4_SEQUENCE=CTCACCAACTAAGCAGCGGT PRIMER_LEFT_4=336,20 PRIMER_RIGHT_4=562,20 PRIMER_LEFT_4_TM=59.779 PRIMER_RIGHT_4_TM=60.453 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=11.1000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=227 PRIMER_PAIR_5_PENALTY=0.690561 PRIMER_LEFT_5_PENALTY=0.237616 PRIMER_RIGHT_5_PENALTY=0.452945 PRIMER_LEFT_5_SEQUENCE=TGGAGGTACCCACTGATGGT PRIMER_RIGHT_5_SEQUENCE=ACTCACCAACTAAGCAGCGG PRIMER_LEFT_5=252,20 PRIMER_RIGHT_5=563,20 PRIMER_LEFT_5_TM=60.238 PRIMER_RIGHT_5_TM=60.453 PRIMER_LEFT_5_GC_PERCENT=55.000 PRIMER_RIGHT_5_GC_PERCENT=55.000 PRIMER_LEFT_5_SELF_ANY=6.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=2.00 PRIMER_LEFT_5_END_STABILITY=7.8000 PRIMER_RIGHT_5_END_STABILITY=11.4000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PAIR_5_PRODUCT_SIZE=312 PRIMER_PAIR_6_PENALTY=0.840127 PRIMER_LEFT_6_PENALTY=0.327840 PRIMER_RIGHT_6_PENALTY=0.512286 PRIMER_LEFT_6_SEQUENCE=GTGGCAGACTGAACATGCAC PRIMER_RIGHT_6_SEQUENCE=TCACCAACTAAGCAGCGGTA PRIMER_LEFT_6=312,20 PRIMER_RIGHT_6=561,20 PRIMER_LEFT_6_TM=60.328 PRIMER_RIGHT_6_TM=59.488 PRIMER_LEFT_6_GC_PERCENT=55.000 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_LEFT_6_SELF_ANY=5.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_END_STABILITY=8.2000 PRIMER_RIGHT_6_END_STABILITY=8.9000 PRIMER_PAIR_6_COMPL_ANY=3.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PAIR_6_PRODUCT_SIZE=250 PRIMER_PAIR_7_PENALTY=0.916059 PRIMER_LEFT_7_PENALTY=0.203367 PRIMER_RIGHT_7_PENALTY=0.712692 PRIMER_LEFT_7_SEQUENCE=GGACCAAAACCTGCATTGAT PRIMER_RIGHT_7_SEQUENCE=CACACCTCTCGAACCACCTC PRIMER_LEFT_7=372,20 PRIMER_RIGHT_7=1071,20 PRIMER_LEFT_7_TM=59.797 PRIMER_RIGHT_7_TM=60.713 PRIMER_LEFT_7_GC_PERCENT=45.000 PRIMER_RIGHT_7_GC_PERCENT=60.000 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=6.9000 PRIMER_RIGHT_7_END_STABILITY=7.6000 PRIMER_PAIR_7_COMPL_ANY=2.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PAIR_7_PRODUCT_SIZE=700 PRIMER_PAIR_8_PENALTY=1.179338 PRIMER_LEFT_8_PENALTY=1.139192 PRIMER_RIGHT_8_PENALTY=0.040146 PRIMER_LEFT_8_SEQUENCE=TGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_8_SEQUENCE=ACATCCGCCGTAAAAGAATG PRIMER_LEFT_8=416,21 PRIMER_RIGHT_8=1171,20 PRIMER_LEFT_8_TM=59.861 PRIMER_RIGHT_8_TM=59.960 PRIMER_LEFT_8_GC_PERCENT=47.619 PRIMER_RIGHT_8_GC_PERCENT=45.000 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=3.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_LEFT_8_END_STABILITY=7.0000 PRIMER_RIGHT_8_END_STABILITY=6.9000 PRIMER_PAIR_8_COMPL_ANY=3.00 PRIMER_PAIR_8_COMPL_END=0.00 PRIMER_PAIR_8_PRODUCT_SIZE=756 PRIMER_PAIR_9_PENALTY=1.179338 PRIMER_LEFT_9_PENALTY=1.139192 PRIMER_RIGHT_9_PENALTY=0.040146 PRIMER_LEFT_9_SEQUENCE=TTGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_9_SEQUENCE=CACATCCGCCGTAAAAGAAT PRIMER_LEFT_9=415,21 PRIMER_RIGHT_9=1172,20 PRIMER_LEFT_9_TM=59.861 PRIMER_RIGHT_9_TM=59.960 PRIMER_LEFT_9_GC_PERCENT=47.619 PRIMER_RIGHT_9_GC_PERCENT=45.000 PRIMER_LEFT_9_SELF_ANY=5.00 PRIMER_RIGHT_9_SELF_ANY=3.00 PRIMER_LEFT_9_SELF_END=1.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_END_STABILITY=8.2000 PRIMER_RIGHT_9_END_STABILITY=6.6000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=758 PRIMER_PAIR_10_PENALTY=1.248189 PRIMER_LEFT_10_PENALTY=0.376749 PRIMER_RIGHT_10_PENALTY=0.871441 PRIMER_LEFT_10_SEQUENCE=CTGGAGGTACCCACTGATGG PRIMER_RIGHT_10_SEQUENCE=TCAGAGCACACCTCTCGAAC PRIMER_LEFT_10=251,20 PRIMER_RIGHT_10=1077,20 PRIMER_LEFT_10_TM=60.377 PRIMER_RIGHT_10_TM=59.129 PRIMER_LEFT_10_GC_PERCENT=60.000 PRIMER_RIGHT_10_GC_PERCENT=55.000 PRIMER_LEFT_10_SELF_ANY=6.00 PRIMER_RIGHT_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_END_STABILITY=8.1000 PRIMER_RIGHT_10_END_STABILITY=8.4000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=827 PRIMER_PAIR_11_PENALTY=1.293286 PRIMER_LEFT_11_PENALTY=0.420643 PRIMER_RIGHT_11_PENALTY=0.872644 PRIMER_LEFT_11_SEQUENCE=ACTGATGGTAATGCTGGCCT PRIMER_RIGHT_11_SEQUENCE=GAGCACACCTCTCGAACCAC PRIMER_LEFT_11=263,20 PRIMER_RIGHT_11=1074,20 PRIMER_LEFT_11_TM=59.579 PRIMER_RIGHT_11_TM=60.873 PRIMER_LEFT_11_GC_PERCENT=50.000 PRIMER_RIGHT_11_GC_PERCENT=60.000 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_END_STABILITY=10.9000 PRIMER_RIGHT_11_END_STABILITY=7.6000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=0.00 PRIMER_PAIR_11_PRODUCT_SIZE=812 PRIMER_PAIR_12_PENALTY=1.467010 PRIMER_LEFT_12_PENALTY=0.466916 PRIMER_RIGHT_12_PENALTY=1.000094 PRIMER_LEFT_12_SEQUENCE=GCATTGATACCAAGGAAGGC PRIMER_RIGHT_12_SEQUENCE=ACTGCATGTCTCTTTGGCG PRIMER_LEFT_12=384,20 PRIMER_RIGHT_12=676,19 PRIMER_LEFT_12_TM=59.533 PRIMER_RIGHT_12_TM=60.000 PRIMER_LEFT_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_GC_PERCENT=52.632 PRIMER_LEFT_12_SELF_ANY=6.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_END_STABILITY=9.7000 PRIMER_RIGHT_12_END_STABILITY=11.7000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PAIR_12_PRODUCT_SIZE=293 PRIMER_PAIR_13_PENALTY=1.482050 PRIMER_LEFT_13_PENALTY=0.473276 PRIMER_RIGHT_13_PENALTY=1.008773 PRIMER_LEFT_13_SEQUENCE=CTGTGGCAGACTGAACATGC PRIMER_RIGHT_13_SEQUENCE=AGAGCACACCTCTCGAACCA PRIMER_LEFT_13=310,20 PRIMER_RIGHT_13=1075,20 PRIMER_LEFT_13_TM=60.473 PRIMER_RIGHT_13_TM=61.009 PRIMER_LEFT_13_GC_PERCENT=55.000 PRIMER_RIGHT_13_GC_PERCENT=55.000 PRIMER_LEFT_13_SELF_ANY=5.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=0.00 PRIMER_LEFT_13_END_STABILITY=8.4000 PRIMER_RIGHT_13_END_STABILITY=8.2000 PRIMER_PAIR_13_COMPL_ANY=4.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PAIR_13_PRODUCT_SIZE=766 PRIMER_PAIR_14_PENALTY=1.606490 PRIMER_LEFT_14_PENALTY=1.558232 PRIMER_RIGHT_14_PENALTY=0.048259 PRIMER_LEFT_14_SEQUENCE=TGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_14_SEQUENCE=TTCTCATCCCCAGGTGTCTC PRIMER_LEFT_14=416,20 PRIMER_RIGHT_14=1356,20 PRIMER_LEFT_14_TM=58.442 PRIMER_RIGHT_14_TM=60.048 PRIMER_LEFT_14_GC_PERCENT=50.000 PRIMER_RIGHT_14_GC_PERCENT=55.000 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=3.00 PRIMER_LEFT_14_SELF_END=1.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_END_STABILITY=8.2000 PRIMER_RIGHT_14_END_STABILITY=6.1000 PRIMER_PAIR_14_COMPL_ANY=4.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PAIR_14_PRODUCT_SIZE=941 PRIMER_PAIR_15_PENALTY=1.672321 PRIMER_LEFT_15_PENALTY=0.663548 PRIMER_RIGHT_15_PENALTY=1.008773 PRIMER_LEFT_15_SEQUENCE=AGATCCATCAGGGACCAAAA PRIMER_RIGHT_15_SEQUENCE=CAGAGCACACCTCTCGAACC PRIMER_LEFT_15=361,20 PRIMER_RIGHT_15=1076,20 PRIMER_LEFT_15_TM=59.336 PRIMER_RIGHT_15_TM=61.009 PRIMER_LEFT_15_GC_PERCENT=45.000 PRIMER_RIGHT_15_GC_PERCENT=60.000 PRIMER_LEFT_15_SELF_ANY=5.00 PRIMER_RIGHT_15_SELF_ANY=4.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_END_STABILITY=7.6000 PRIMER_RIGHT_15_END_STABILITY=7.9000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PAIR_15_PRODUCT_SIZE=716 PRIMER_PAIR_16_PENALTY=1.692682 PRIMER_LEFT_16_PENALTY=0.663548 PRIMER_RIGHT_16_PENALTY=1.029134 PRIMER_LEFT_16_SEQUENCE=AATGGGAAGTGGGATTCAGA PRIMER_RIGHT_16_SEQUENCE=GAGCACACCTCTCGAACCA PRIMER_LEFT_16=344,20 PRIMER_RIGHT_16=1074,19 PRIMER_LEFT_16_TM=59.336 PRIMER_RIGHT_16_TM=59.971 PRIMER_LEFT_16_GC_PERCENT=45.000 PRIMER_RIGHT_16_GC_PERCENT=57.895 PRIMER_LEFT_16_SELF_ANY=3.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_END_STABILITY=6.7000 PRIMER_RIGHT_16_END_STABILITY=8.2000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=3.00 PRIMER_PAIR_16_PRODUCT_SIZE=731 PRIMER_PAIR_17_PENALTY=2.005503 PRIMER_LEFT_17_PENALTY=0.807658 PRIMER_RIGHT_17_PENALTY=1.197845 PRIMER_LEFT_17_SEQUENCE=GATCCATCAGGGACCAAAAC PRIMER_RIGHT_17_SEQUENCE=TTGTCTTCACTCCCATCTGC PRIMER_LEFT_17=362,20 PRIMER_RIGHT_17=870,20 PRIMER_LEFT_17_TM=59.192 PRIMER_RIGHT_17_TM=58.802 PRIMER_LEFT_17_GC_PERCENT=50.000 PRIMER_RIGHT_17_GC_PERCENT=50.000 PRIMER_LEFT_17_SELF_ANY=5.00 PRIMER_RIGHT_17_SELF_ANY=2.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_END_STABILITY=7.0000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=4.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PAIR_17_PRODUCT_SIZE=509 PRIMER_PAIR_18_PENALTY=2.063532 PRIMER_LEFT_18_PENALTY=0.663548 PRIMER_RIGHT_18_PENALTY=1.399985 PRIMER_LEFT_18_SEQUENCE=GAATGGGAAGTGGGATTCAG PRIMER_RIGHT_18_SEQUENCE=CTCACCAACTAAGCAGCGG PRIMER_LEFT_18=343,20 PRIMER_RIGHT_18=562,19 PRIMER_LEFT_18_TM=59.336 PRIMER_RIGHT_18_TM=59.600 PRIMER_LEFT_18_GC_PERCENT=50.000 PRIMER_RIGHT_18_GC_PERCENT=57.895 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=3.00 PRIMER_LEFT_18_SELF_END=1.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_END_STABILITY=7.0000 PRIMER_RIGHT_18_END_STABILITY=11.4000 PRIMER_PAIR_18_COMPL_ANY=4.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PAIR_18_PRODUCT_SIZE=220 PRIMER_PAIR_19_PENALTY=2.075759 PRIMER_LEFT_19_PENALTY=0.828193 PRIMER_RIGHT_19_PENALTY=1.247566 PRIMER_LEFT_19_SEQUENCE=TGGGATTCAGATCCATCAGG PRIMER_RIGHT_19_SEQUENCE=TTTGTCTTCACTCCCATCTGC PRIMER_LEFT_19=353,20 PRIMER_RIGHT_19=871,21 PRIMER_LEFT_19_TM=60.828 PRIMER_RIGHT_19_TM=60.248 PRIMER_LEFT_19_GC_PERCENT=50.000 PRIMER_RIGHT_19_GC_PERCENT=47.619 PRIMER_LEFT_19_SELF_ANY=5.00 PRIMER_RIGHT_19_SELF_ANY=2.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_END_STABILITY=8.2000 PRIMER_RIGHT_19_END_STABILITY=8.2000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=1.00 PRIMER_PAIR_19_PRODUCT_SIZE=519 PRIMER_PAIR_20_PENALTY=2.092619 PRIMER_LEFT_20_PENALTY=0.663548 PRIMER_RIGHT_20_PENALTY=1.429072 PRIMER_LEFT_20_SEQUENCE=AGAATGGGAAGTGGGATTCA PRIMER_RIGHT_20_SEQUENCE=AAACTCACCAACTAAGCAGCG PRIMER_LEFT_20=342,20 PRIMER_RIGHT_20=565,21 PRIMER_LEFT_20_TM=59.336 PRIMER_RIGHT_20_TM=59.571 PRIMER_LEFT_20_GC_PERCENT=45.000 PRIMER_RIGHT_20_GC_PERCENT=47.619 PRIMER_LEFT_20_SELF_ANY=4.00 PRIMER_RIGHT_20_SELF_ANY=3.00 PRIMER_LEFT_20_SELF_END=1.00 PRIMER_RIGHT_20_SELF_END=2.00 PRIMER_LEFT_20_END_STABILITY=6.9000 PRIMER_RIGHT_20_END_STABILITY=10.2000 PRIMER_PAIR_20_COMPL_ANY=4.00 PRIMER_PAIR_20_COMPL_END=0.00 PRIMER_PAIR_20_PRODUCT_SIZE=224 PRIMER_PAIR_21_PENALTY=2.108403 PRIMER_LEFT_21_PENALTY=2.047791 PRIMER_RIGHT_21_PENALTY=0.060612 PRIMER_LEFT_21_SEQUENCE=TTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_21_SEQUENCE=GTTTCGCAAACATCCATCCT PRIMER_LEFT_21=415,20 PRIMER_RIGHT_21=636,20 PRIMER_LEFT_21_TM=57.952 PRIMER_RIGHT_21_TM=59.939 PRIMER_LEFT_21_GC_PERCENT=50.000 PRIMER_RIGHT_21_GC_PERCENT=45.000 PRIMER_LEFT_21_SELF_ANY=5.00 PRIMER_RIGHT_21_SELF_ANY=6.00 PRIMER_LEFT_21_SELF_END=1.00 PRIMER_RIGHT_21_SELF_END=0.00 PRIMER_LEFT_21_END_STABILITY=9.7000 PRIMER_RIGHT_21_END_STABILITY=7.8000 PRIMER_PAIR_21_COMPL_ANY=5.00 PRIMER_PAIR_21_COMPL_END=0.00 PRIMER_PAIR_21_PRODUCT_SIZE=222 PRIMER_PAIR_22_PENALTY=2.110097 PRIMER_LEFT_22_PENALTY=0.663548 PRIMER_RIGHT_22_PENALTY=1.446550 PRIMER_LEFT_22_SEQUENCE=CAGAATGGGAAGTGGGATTC PRIMER_RIGHT_22_SEQUENCE=TCACCAACTAAGCAGCGGTAG PRIMER_LEFT_22=341,20 PRIMER_RIGHT_22=561,21 PRIMER_LEFT_22_TM=59.336 PRIMER_RIGHT_22_TM=60.447 PRIMER_LEFT_22_GC_PERCENT=50.000 PRIMER_RIGHT_22_GC_PERCENT=52.381 PRIMER_LEFT_22_SELF_ANY=4.00 PRIMER_RIGHT_22_SELF_ANY=3.00 PRIMER_LEFT_22_SELF_END=3.00 PRIMER_RIGHT_22_SELF_END=3.00 PRIMER_LEFT_22_END_STABILITY=6.6000 PRIMER_RIGHT_22_END_STABILITY=6.9000 PRIMER_PAIR_22_COMPL_ANY=4.00 PRIMER_PAIR_22_COMPL_END=1.00 PRIMER_PAIR_22_PRODUCT_SIZE=221 PRIMER_PAIR_23_PENALTY=2.150754 PRIMER_LEFT_23_PENALTY=0.704204 PRIMER_RIGHT_23_PENALTY=1.446550 PRIMER_LEFT_23_SEQUENCE=CATGAATGTCCAGAATGGGA PRIMER_RIGHT_23_SEQUENCE=CTCACCAACTAAGCAGCGGTA PRIMER_LEFT_23=331,20 PRIMER_RIGHT_23=562,21 PRIMER_LEFT_23_TM=59.296 PRIMER_RIGHT_23_TM=60.447 PRIMER_LEFT_23_GC_PERCENT=45.000 PRIMER_RIGHT_23_GC_PERCENT=52.381 PRIMER_LEFT_23_SELF_ANY=4.00 PRIMER_RIGHT_23_SELF_ANY=3.00 PRIMER_LEFT_23_SELF_END=1.00 PRIMER_RIGHT_23_SELF_END=2.00 PRIMER_LEFT_23_END_STABILITY=9.7000 PRIMER_RIGHT_23_END_STABILITY=8.9000 PRIMER_PAIR_23_COMPL_ANY=3.00 PRIMER_PAIR_23_COMPL_END=0.00 PRIMER_PAIR_23_PRODUCT_SIZE=232 PRIMER_PAIR_24_PENALTY=2.190969 PRIMER_LEFT_24_PENALTY=2.130358 PRIMER_RIGHT_24_PENALTY=0.060612 PRIMER_LEFT_24_SEQUENCE=ATTGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_24_SEQUENCE=AGTTTCGCAAACATCCATCC PRIMER_LEFT_24=414,22 PRIMER_RIGHT_24=637,20 PRIMER_LEFT_24_TM=60.130 PRIMER_RIGHT_24_TM=59.939 PRIMER_LEFT_24_GC_PERCENT=45.455 PRIMER_RIGHT_24_GC_PERCENT=45.000 PRIMER_LEFT_24_SELF_ANY=5.00 PRIMER_RIGHT_24_SELF_ANY=6.00 PRIMER_LEFT_24_SELF_END=1.00 PRIMER_RIGHT_24_SELF_END=0.00 PRIMER_LEFT_24_END_STABILITY=8.2000 PRIMER_RIGHT_24_END_STABILITY=8.1000 PRIMER_PAIR_24_COMPL_ANY=5.00 PRIMER_PAIR_24_COMPL_END=1.00 PRIMER_PAIR_24_PRODUCT_SIZE=224 PRIMER_PAIR_25_PENALTY=2.333956 PRIMER_LEFT_25_PENALTY=2.096340 PRIMER_RIGHT_25_PENALTY=0.237616 PRIMER_LEFT_25_SEQUENCE=ATCAGCTGACTCGCCTGG PRIMER_RIGHT_25_SEQUENCE=ACCATCAGTGGGTACCTCCA PRIMER_LEFT_25=2,18 PRIMER_RIGHT_25=271,20 PRIMER_LEFT_25_TM=60.096 PRIMER_RIGHT_25_TM=60.238 PRIMER_LEFT_25_GC_PERCENT=61.111 PRIMER_RIGHT_25_GC_PERCENT=55.000 PRIMER_LEFT_25_SELF_ANY=8.00 PRIMER_RIGHT_25_SELF_ANY=6.00 PRIMER_LEFT_25_SELF_END=3.00 PRIMER_RIGHT_25_SELF_END=2.00 PRIMER_LEFT_25_END_STABILITY=9.7000 PRIMER_RIGHT_25_END_STABILITY=8.2000 PRIMER_PAIR_25_COMPL_ANY=4.00 PRIMER_PAIR_25_COMPL_END=3.00 PRIMER_PAIR_25_PRODUCT_SIZE=270 PRIMER_PAIR_26_PENALTY=2.396276 PRIMER_LEFT_26_PENALTY=0.923785 PRIMER_RIGHT_26_PENALTY=1.472491 PRIMER_LEFT_26_SEQUENCE=CCCAGATTGCCATGTTCTGT PRIMER_RIGHT_26_SEQUENCE=TAAACTTTGGGACATGGCG PRIMER_LEFT_26=294,20 PRIMER_RIGHT_26=1246,19 PRIMER_LEFT_26_TM=60.924 PRIMER_RIGHT_26_TM=59.528 PRIMER_LEFT_26_GC_PERCENT=50.000 PRIMER_RIGHT_26_GC_PERCENT=47.368 PRIMER_LEFT_26_SELF_ANY=4.00 PRIMER_RIGHT_26_SELF_ANY=5.00 PRIMER_LEFT_26_SELF_END=0.00 PRIMER_RIGHT_26_SELF_END=2.00 PRIMER_LEFT_26_END_STABILITY=6.4000 PRIMER_RIGHT_26_END_STABILITY=11.7000 PRIMER_PAIR_26_COMPL_ANY=7.00 PRIMER_PAIR_26_COMPL_END=3.00 PRIMER_PAIR_26_PRODUCT_SIZE=953 PRIMER_PAIR_27_PENALTY=2.405067 PRIMER_LEFT_27_PENALTY=0.860239 PRIMER_RIGHT_27_PENALTY=1.544828 PRIMER_LEFT_27_SEQUENCE=CAGATCCATCAGGGACCAAA PRIMER_RIGHT_27_SEQUENCE=TCACCAACTAAGCAGCGGT PRIMER_LEFT_27=360,20 PRIMER_RIGHT_27=561,19 PRIMER_LEFT_27_TM=60.860 PRIMER_RIGHT_27_TM=59.455 PRIMER_LEFT_27_GC_PERCENT=50.000 PRIMER_RIGHT_27_GC_PERCENT=52.632 PRIMER_LEFT_27_SELF_ANY=5.00 PRIMER_RIGHT_27_SELF_ANY=3.00 PRIMER_LEFT_27_SELF_END=0.00 PRIMER_RIGHT_27_SELF_END=2.00 PRIMER_LEFT_27_END_STABILITY=8.8000 PRIMER_RIGHT_27_END_STABILITY=11.1000 PRIMER_PAIR_27_COMPL_ANY=3.00 PRIMER_PAIR_27_COMPL_END=1.00 PRIMER_PAIR_27_PRODUCT_SIZE=202 PRIMER_PAIR_28_PENALTY=2.438330 PRIMER_LEFT_28_PENALTY=2.413667 PRIMER_RIGHT_28_PENALTY=0.024663 PRIMER_LEFT_28_SEQUENCE=CGCTGGAGGTACCCACTGAT PRIMER_RIGHT_28_SEQUENCE=TTGCACTTGTCAGGAACGAG PRIMER_LEFT_28=249,20 PRIMER_RIGHT_28=600,20 PRIMER_LEFT_28_TM=62.414 PRIMER_RIGHT_28_TM=60.025 PRIMER_LEFT_28_GC_PERCENT=60.000 PRIMER_RIGHT_28_GC_PERCENT=50.000 PRIMER_LEFT_28_SELF_ANY=6.00 PRIMER_RIGHT_28_SELF_ANY=4.00 PRIMER_LEFT_28_SELF_END=2.00 PRIMER_RIGHT_28_SELF_END=2.00 PRIMER_LEFT_28_END_STABILITY=6.6000 PRIMER_RIGHT_28_END_STABILITY=8.1000 PRIMER_PAIR_28_COMPL_ANY=4.00 PRIMER_PAIR_28_COMPL_END=1.00 PRIMER_PAIR_28_PRODUCT_SIZE=352 PRIMER_PAIR_29_PENALTY=2.532768 PRIMER_LEFT_29_PENALTY=0.949861 PRIMER_RIGHT_29_PENALTY=1.582907 PRIMER_LEFT_29_SEQUENCE=ATCCATCAGGGACCAAAACC PRIMER_RIGHT_29_SEQUENCE=TTCAGAGCACACCTCTCGAAC PRIMER_LEFT_29=363,20 PRIMER_RIGHT_29=1078,21 PRIMER_LEFT_29_TM=60.950 PRIMER_RIGHT_29_TM=60.583 PRIMER_LEFT_29_GC_PERCENT=50.000 PRIMER_RIGHT_29_GC_PERCENT=52.381 PRIMER_LEFT_29_SELF_ANY=5.00 PRIMER_RIGHT_29_SELF_ANY=5.00 PRIMER_LEFT_29_SELF_END=0.00 PRIMER_RIGHT_29_SELF_END=1.00 PRIMER_LEFT_29_END_STABILITY=8.2000 PRIMER_RIGHT_29_END_STABILITY=8.4000 PRIMER_PAIR_29_COMPL_ANY=3.00 PRIMER_PAIR_29_COMPL_END=0.00 PRIMER_PAIR_29_PRODUCT_SIZE=716 PRIMER_PAIR_30_PENALTY=2.686568 PRIMER_LEFT_30_PENALTY=1.014189 PRIMER_RIGHT_30_PENALTY=1.672380 PRIMER_LEFT_30_SEQUENCE=TCAGATCCATCAGGGACCA PRIMER_RIGHT_30_SEQUENCE=TGTCTTCACTCCCATCTGCAT PRIMER_LEFT_30=359,19 PRIMER_RIGHT_30=869,21 PRIMER_LEFT_30_TM=59.986 PRIMER_RIGHT_30_TM=60.672 PRIMER_LEFT_30_GC_PERCENT=52.632 PRIMER_RIGHT_30_GC_PERCENT=47.619 PRIMER_LEFT_30_SELF_ANY=5.00 PRIMER_RIGHT_30_SELF_ANY=4.00 PRIMER_LEFT_30_SELF_END=3.00 PRIMER_RIGHT_30_SELF_END=2.00 PRIMER_LEFT_30_END_STABILITY=7.9000 PRIMER_RIGHT_30_END_STABILITY=8.4000 PRIMER_PAIR_30_COMPL_ANY=5.00 PRIMER_PAIR_30_COMPL_END=1.00 PRIMER_PAIR_30_PRODUCT_SIZE=511 PRIMER_PAIR_31_PENALTY=2.731923 PRIMER_LEFT_31_PENALTY=2.199316 PRIMER_RIGHT_31_PENALTY=0.532607 PRIMER_LEFT_31_SEQUENCE=GGGCTCCGTCAGTTTCCT PRIMER_RIGHT_31_SEQUENCE=CATCAGTGGGTACCTCCAGC PRIMER_LEFT_31=43,18 PRIMER_RIGHT_31=269,20 PRIMER_LEFT_31_TM=60.199 PRIMER_RIGHT_31_TM=60.533 PRIMER_LEFT_31_GC_PERCENT=61.111 PRIMER_RIGHT_31_GC_PERCENT=60.000 PRIMER_LEFT_31_SELF_ANY=2.00 PRIMER_RIGHT_31_SELF_ANY=6.00 PRIMER_LEFT_31_SELF_END=0.00 PRIMER_RIGHT_31_SELF_END=2.00 PRIMER_LEFT_31_END_STABILITY=8.2000 PRIMER_RIGHT_31_END_STABILITY=9.7000 PRIMER_PAIR_31_COMPL_ANY=3.00 PRIMER_PAIR_31_COMPL_END=1.00 PRIMER_PAIR_31_PRODUCT_SIZE=227 PRIMER_PAIR_32_PENALTY=2.732951 PRIMER_LEFT_32_PENALTY=2.660713 PRIMER_RIGHT_32_PENALTY=0.072238 PRIMER_LEFT_32_SEQUENCE=TGCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_32_SEQUENCE=CCAGACATCCGAGTCATCCT PRIMER_LEFT_32=416,22 PRIMER_RIGHT_32=826,20 PRIMER_LEFT_32_TM=60.661 PRIMER_RIGHT_32_TM=60.072 PRIMER_LEFT_32_GC_PERCENT=50.000 PRIMER_RIGHT_32_GC_PERCENT=55.000 PRIMER_LEFT_32_SELF_ANY=4.00 PRIMER_RIGHT_32_SELF_ANY=5.00 PRIMER_LEFT_32_SELF_END=0.00 PRIMER_RIGHT_32_SELF_END=0.00 PRIMER_LEFT_32_END_STABILITY=6.7000 PRIMER_RIGHT_32_END_STABILITY=7.8000 PRIMER_PAIR_32_COMPL_ANY=5.00 PRIMER_PAIR_32_COMPL_END=0.00 PRIMER_PAIR_32_PRODUCT_SIZE=411 PRIMER_PAIR_33_PENALTY=2.741077 PRIMER_LEFT_33_PENALTY=2.668729 PRIMER_RIGHT_33_PENALTY=0.072349 PRIMER_LEFT_33_SEQUENCE=ATTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_33_SEQUENCE=ACCAGACATCCGAGTCATCC PRIMER_LEFT_33=414,21 PRIMER_RIGHT_33=827,20 PRIMER_LEFT_33_TM=58.331 PRIMER_RIGHT_33_TM=59.928 PRIMER_LEFT_33_GC_PERCENT=47.619 PRIMER_RIGHT_33_GC_PERCENT=55.000 PRIMER_LEFT_33_SELF_ANY=5.00 PRIMER_RIGHT_33_SELF_ANY=5.00 PRIMER_LEFT_33_SELF_END=1.00 PRIMER_RIGHT_33_SELF_END=0.00 PRIMER_LEFT_33_END_STABILITY=9.7000 PRIMER_RIGHT_33_END_STABILITY=8.1000 PRIMER_PAIR_33_COMPL_ANY=5.00 PRIMER_PAIR_33_COMPL_END=1.00 PRIMER_PAIR_33_PRODUCT_SIZE=414 PRIMER_PAIR_34_PENALTY=2.868697 PRIMER_LEFT_34_PENALTY=1.055809 PRIMER_RIGHT_34_PENALTY=1.812888 PRIMER_LEFT_34_SEQUENCE=CCCAGATTGCCATGTTCTG PRIMER_RIGHT_34_SEQUENCE=AACTCACCAACTAAGCAGCG PRIMER_LEFT_34=294,19 PRIMER_RIGHT_34=564,20 PRIMER_LEFT_34_TM=60.056 PRIMER_RIGHT_34_TM=58.187 PRIMER_LEFT_34_GC_PERCENT=52.632 PRIMER_RIGHT_34_GC_PERCENT=50.000 PRIMER_LEFT_34_SELF_ANY=4.00 PRIMER_RIGHT_34_SELF_ANY=3.00 PRIMER_LEFT_34_SELF_END=1.00 PRIMER_RIGHT_34_SELF_END=2.00 PRIMER_LEFT_34_END_STABILITY=7.0000 PRIMER_RIGHT_34_END_STABILITY=10.2000 PRIMER_PAIR_34_COMPL_ANY=3.00 PRIMER_PAIR_34_COMPL_END=1.00 PRIMER_PAIR_34_PRODUCT_SIZE=271 PRIMER_PAIR_35_PENALTY=3.022138 PRIMER_LEFT_35_PENALTY=1.071784 PRIMER_RIGHT_35_PENALTY=1.950354 PRIMER_LEFT_35_SEQUENCE=GATTGCCATGTTCTGTGGC PRIMER_RIGHT_35_SEQUENCE=CTGCATGTCTCTTTGGCGAC PRIMER_LEFT_35=298,19 PRIMER_RIGHT_35=675,20 PRIMER_LEFT_35_TM=60.072 PRIMER_RIGHT_35_TM=61.950 PRIMER_LEFT_35_GC_PERCENT=52.632 PRIMER_RIGHT_35_GC_PERCENT=55.000 PRIMER_LEFT_35_SELF_ANY=4.00 PRIMER_RIGHT_35_SELF_ANY=4.00 PRIMER_LEFT_35_SELF_END=2.00 PRIMER_RIGHT_35_SELF_END=2.00 PRIMER_LEFT_35_END_STABILITY=9.4000 PRIMER_RIGHT_35_END_STABILITY=9.6000 PRIMER_PAIR_35_COMPL_ANY=4.00 PRIMER_PAIR_35_COMPL_END=3.00 PRIMER_PAIR_35_PRODUCT_SIZE=378 PRIMER_PAIR_36_PENALTY=3.025966 PRIMER_LEFT_36_PENALTY=1.075611 PRIMER_RIGHT_36_PENALTY=1.950354 PRIMER_LEFT_36_SEQUENCE=CCAGAATGGGAAGTGGGATT PRIMER_RIGHT_36_SEQUENCE=ACTGCATGTCTCTTTGGCGA PRIMER_LEFT_36=340,20 PRIMER_RIGHT_36=676,20 PRIMER_LEFT_36_TM=61.076 PRIMER_RIGHT_36_TM=61.950 PRIMER_LEFT_36_GC_PERCENT=50.000 PRIMER_RIGHT_36_GC_PERCENT=50.000 PRIMER_LEFT_36_SELF_ANY=3.00 PRIMER_RIGHT_36_SELF_ANY=4.00 PRIMER_LEFT_36_SELF_END=1.00 PRIMER_RIGHT_36_SELF_END=2.00 PRIMER_LEFT_36_END_STABILITY=8.1000 PRIMER_RIGHT_36_END_STABILITY=11.4000 PRIMER_PAIR_36_COMPL_ANY=4.00 PRIMER_PAIR_36_COMPL_END=1.00 PRIMER_PAIR_36_PRODUCT_SIZE=337 PRIMER_PAIR_37_PENALTY=3.056272 PRIMER_LEFT_37_PENALTY=1.078278 PRIMER_RIGHT_37_PENALTY=1.977994 PRIMER_LEFT_37_SEQUENCE=AGATTGCCATGTTCTGTGGC PRIMER_RIGHT_37_SEQUENCE=TCTCACTGCATGTCTCTTTGG PRIMER_LEFT_37=297,20 PRIMER_RIGHT_37=680,21 PRIMER_LEFT_37_TM=61.078 PRIMER_RIGHT_37_TM=59.022 PRIMER_LEFT_37_GC_PERCENT=50.000 PRIMER_RIGHT_37_GC_PERCENT=47.619 PRIMER_LEFT_37_SELF_ANY=4.00 PRIMER_RIGHT_37_SELF_ANY=4.00 PRIMER_LEFT_37_SELF_END=2.00 PRIMER_RIGHT_37_SELF_END=0.00 PRIMER_LEFT_37_END_STABILITY=9.4000 PRIMER_RIGHT_37_END_STABILITY=8.8000 PRIMER_PAIR_37_COMPL_ANY=4.00 PRIMER_PAIR_37_COMPL_END=1.00 PRIMER_PAIR_37_PRODUCT_SIZE=384 PRIMER_PAIR_38_PENALTY=3.072600 PRIMER_LEFT_38_PENALTY=1.081589 PRIMER_RIGHT_38_PENALTY=1.991012 PRIMER_LEFT_38_SEQUENCE=ATCAGGGACCAAAACCTGC PRIMER_RIGHT_38_SEQUENCE=CTCACTGCATGTCTCTTTGGC PRIMER_LEFT_38=367,19 PRIMER_RIGHT_38=679,21 PRIMER_LEFT_38_TM=59.918 PRIMER_RIGHT_38_TM=60.991 PRIMER_LEFT_38_GC_PERCENT=52.632 PRIMER_RIGHT_38_GC_PERCENT=52.381 PRIMER_LEFT_38_SELF_ANY=4.00 PRIMER_RIGHT_38_SELF_ANY=4.00 PRIMER_LEFT_38_SELF_END=2.00 PRIMER_RIGHT_38_SELF_END=2.00 PRIMER_LEFT_38_END_STABILITY=9.7000 PRIMER_RIGHT_38_END_STABILITY=10.0000 PRIMER_PAIR_38_COMPL_ANY=5.00 PRIMER_PAIR_38_COMPL_END=3.00 PRIMER_PAIR_38_PRODUCT_SIZE=313 PRIMER_PAIR_39_PENALTY=3.151416 PRIMER_LEFT_39_PENALTY=3.077802 PRIMER_RIGHT_39_PENALTY=0.073614 PRIMER_LEFT_39_SEQUENCE=GCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_39_SEQUENCE=GTTTAACAGGATCTCGGGCA PRIMER_LEFT_39=417,21 PRIMER_RIGHT_39=1289,20 PRIMER_LEFT_39_TM=57.922 PRIMER_RIGHT_39_TM=60.074 PRIMER_LEFT_39_GC_PERCENT=52.381 PRIMER_RIGHT_39_GC_PERCENT=50.000 PRIMER_LEFT_39_SELF_ANY=4.00 PRIMER_RIGHT_39_SELF_ANY=5.00 PRIMER_LEFT_39_SELF_END=0.00 PRIMER_RIGHT_39_SELF_END=0.00 PRIMER_LEFT_39_END_STABILITY=6.7000 PRIMER_RIGHT_39_END_STABILITY=11.2000 PRIMER_PAIR_39_COMPL_ANY=4.00 PRIMER_PAIR_39_COMPL_END=2.00 PRIMER_PAIR_39_PRODUCT_SIZE=873 PRIMER_PAIR_40_PENALTY=3.157622 PRIMER_LEFT_40_PENALTY=3.077974 PRIMER_RIGHT_40_PENALTY=0.079648 PRIMER_LEFT_40_SEQUENCE=GCCAAGAAGTCTACCCTGAACT PRIMER_RIGHT_40_SEQUENCE=CATCCATCCTCTCCTGGTGT PRIMER_LEFT_40=417,22 PRIMER_RIGHT_40=626,20 PRIMER_LEFT_40_TM=58.922 PRIMER_RIGHT_40_TM=59.920 PRIMER_LEFT_40_GC_PERCENT=50.000 PRIMER_RIGHT_40_GC_PERCENT=55.000 PRIMER_LEFT_40_SELF_ANY=4.00 PRIMER_RIGHT_40_SELF_ANY=3.00 PRIMER_LEFT_40_SELF_END=1.00 PRIMER_RIGHT_40_SELF_END=1.00 PRIMER_LEFT_40_END_STABILITY=6.4000 PRIMER_RIGHT_40_END_STABILITY=7.6000 PRIMER_PAIR_40_COMPL_ANY=4.00 PRIMER_PAIR_40_COMPL_END=1.00 PRIMER_PAIR_40_PRODUCT_SIZE=210 PRIMER_PAIR_41_PENALTY=3.212495 PRIMER_LEFT_41_PENALTY=3.132848 PRIMER_RIGHT_41_PENALTY=0.079648 PRIMER_LEFT_41_SEQUENCE=TTGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_41_SEQUENCE=ACATCCATCCTCTCCTGGTG PRIMER_LEFT_41=415,22 PRIMER_RIGHT_41=627,20 PRIMER_LEFT_41_TM=61.133 PRIMER_RIGHT_41_TM=59.920 PRIMER_LEFT_41_GC_PERCENT=45.455 PRIMER_RIGHT_41_GC_PERCENT=55.000 PRIMER_LEFT_41_SELF_ANY=5.00 PRIMER_RIGHT_41_SELF_ANY=3.00 PRIMER_LEFT_41_SELF_END=0.00 PRIMER_RIGHT_41_SELF_END=3.00 PRIMER_LEFT_41_END_STABILITY=7.0000 PRIMER_RIGHT_41_END_STABILITY=8.2000 PRIMER_PAIR_41_COMPL_ANY=4.00 PRIMER_PAIR_41_COMPL_END=1.00 PRIMER_PAIR_41_PRODUCT_SIZE=213 PRIMER_PAIR_42_PENALTY=3.239151 PRIMER_LEFT_42_PENALTY=3.135712 PRIMER_RIGHT_42_PENALTY=0.103439 PRIMER_LEFT_42_SEQUENCE=TATTGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_42_SEQUENCE=CTACCCCTCGGAACTTGTCA PRIMER_LEFT_42=413,23 PRIMER_RIGHT_42=746,20 PRIMER_LEFT_42_TM=60.136 PRIMER_RIGHT_42_TM=60.103 PRIMER_LEFT_42_GC_PERCENT=43.478 PRIMER_RIGHT_42_GC_PERCENT=55.000 PRIMER_LEFT_42_SELF_ANY=5.00 PRIMER_RIGHT_42_SELF_ANY=3.00 PRIMER_LEFT_42_SELF_END=1.00 PRIMER_RIGHT_42_SELF_END=3.00 PRIMER_LEFT_42_END_STABILITY=8.2000 PRIMER_RIGHT_42_END_STABILITY=6.7000 PRIMER_PAIR_42_COMPL_ANY=5.00 PRIMER_PAIR_42_COMPL_END=3.00 PRIMER_PAIR_42_PRODUCT_SIZE=334 PRIMER_PAIR_43_PENALTY=3.271637 PRIMER_LEFT_43_PENALTY=1.147302 PRIMER_RIGHT_43_PENALTY=2.124336 PRIMER_LEFT_43_SEQUENCE=GGAAGTGGGATTCAGATCCAT PRIMER_RIGHT_43_SEQUENCE=CTGCATGTCTCTTTGGCGA PRIMER_LEFT_43=348,21 PRIMER_RIGHT_43=675,19 PRIMER_LEFT_43_TM=60.147 PRIMER_RIGHT_43_TM=61.124 PRIMER_LEFT_43_GC_PERCENT=47.619 PRIMER_RIGHT_43_GC_PERCENT=52.632 PRIMER_LEFT_43_SELF_ANY=5.00 PRIMER_RIGHT_43_SELF_ANY=4.00 PRIMER_LEFT_43_SELF_END=2.00 PRIMER_RIGHT_43_SELF_END=2.00 PRIMER_LEFT_43_END_STABILITY=8.1000 PRIMER_RIGHT_43_END_STABILITY=11.4000 PRIMER_PAIR_43_COMPL_ANY=5.00 PRIMER_PAIR_43_COMPL_END=1.00 PRIMER_PAIR_43_PRODUCT_SIZE=328 PRIMER_PAIR_44_PENALTY=3.354296 PRIMER_LEFT_44_PENALTY=3.250857 PRIMER_RIGHT_44_PENALTY=0.103439 PRIMER_LEFT_44_SEQUENCE=GCGCTGGAGGTACCCACT PRIMER_RIGHT_44_SEQUENCE=CACAAACTCTACCCCTCGGA PRIMER_LEFT_44=248,18 PRIMER_RIGHT_44=754,20 PRIMER_LEFT_44_TM=61.251 PRIMER_RIGHT_44_TM=60.103 PRIMER_LEFT_44_GC_PERCENT=66.667 PRIMER_RIGHT_44_GC_PERCENT=55.000 PRIMER_LEFT_44_SELF_ANY=6.00 PRIMER_RIGHT_44_SELF_ANY=3.00 PRIMER_LEFT_44_SELF_END=2.00 PRIMER_RIGHT_44_SELF_END=3.00 PRIMER_LEFT_44_END_STABILITY=7.9000 PRIMER_RIGHT_44_END_STABILITY=9.9000 PRIMER_PAIR_44_COMPL_ANY=4.00 PRIMER_PAIR_44_COMPL_END=1.00 PRIMER_PAIR_44_PRODUCT_SIZE=507 PRIMER_PAIR_45_PENALTY=3.362562 PRIMER_LEFT_45_PENALTY=1.183296 PRIMER_RIGHT_45_PENALTY=2.179265 PRIMER_LEFT_45_SEQUENCE=AGATCCATCAGGGACCAAAAC PRIMER_RIGHT_45_SEQUENCE=AGCACACCTCTCGAACCAC PRIMER_LEFT_45=361,21 PRIMER_RIGHT_45=1073,19 PRIMER_LEFT_45_TM=60.183 PRIMER_RIGHT_45_TM=58.821 PRIMER_LEFT_45_GC_PERCENT=47.619 PRIMER_RIGHT_45_GC_PERCENT=57.895 PRIMER_LEFT_45_SELF_ANY=5.00 PRIMER_RIGHT_45_SELF_ANY=4.00 PRIMER_LEFT_45_SELF_END=0.00 PRIMER_RIGHT_45_SELF_END=0.00 PRIMER_LEFT_45_END_STABILITY=7.0000 PRIMER_RIGHT_45_END_STABILITY=7.6000 PRIMER_PAIR_45_COMPL_ANY=3.00 PRIMER_PAIR_45_COMPL_END=0.00 PRIMER_PAIR_45_PRODUCT_SIZE=713 PRIMER_PAIR_46_PENALTY=3.398150 PRIMER_LEFT_46_PENALTY=3.255899 PRIMER_RIGHT_46_PENALTY=0.142251 PRIMER_LEFT_46_SEQUENCE=CGCTGGAGGTACCCACTGA PRIMER_RIGHT_46_SEQUENCE=AACATGCCGTAGTCATGCAA PRIMER_LEFT_46=249,19 PRIMER_RIGHT_46=711,20 PRIMER_LEFT_46_TM=62.256 PRIMER_RIGHT_46_TM=60.142 PRIMER_LEFT_46_GC_PERCENT=63.158 PRIMER_RIGHT_46_GC_PERCENT=45.000 PRIMER_LEFT_46_SELF_ANY=6.00 PRIMER_RIGHT_46_SELF_ANY=6.00 PRIMER_LEFT_46_SELF_END=2.00 PRIMER_RIGHT_46_SELF_END=2.00 PRIMER_LEFT_46_END_STABILITY=6.4000 PRIMER_RIGHT_46_END_STABILITY=8.8000 PRIMER_PAIR_46_COMPL_ANY=3.00 PRIMER_PAIR_46_COMPL_END=0.00 PRIMER_PAIR_46_PRODUCT_SIZE=463 PRIMER_PAIR_47_PENALTY=3.428448 PRIMER_LEFT_47_PENALTY=1.188029 PRIMER_RIGHT_47_PENALTY=2.240419 PRIMER_LEFT_47_SEQUENCE=ACATGAATGTCCAGAATGGGA PRIMER_RIGHT_47_SEQUENCE=ACTCACCAACTAAGCAGCGGT PRIMER_LEFT_47=330,21 PRIMER_RIGHT_47=563,21 PRIMER_LEFT_47_TM=60.188 PRIMER_RIGHT_47_TM=61.240 PRIMER_LEFT_47_GC_PERCENT=42.857 PRIMER_RIGHT_47_GC_PERCENT=52.381 PRIMER_LEFT_47_SELF_ANY=6.00 PRIMER_RIGHT_47_SELF_ANY=3.00 PRIMER_LEFT_47_SELF_END=1.00 PRIMER_RIGHT_47_SELF_END=2.00 PRIMER_LEFT_47_END_STABILITY=9.7000 PRIMER_RIGHT_47_END_STABILITY=11.1000 PRIMER_PAIR_47_COMPL_ANY=3.00 PRIMER_PAIR_47_COMPL_END=1.00 PRIMER_PAIR_47_PRODUCT_SIZE=234 PRIMER_PAIR_48_PENALTY=3.442199 PRIMER_LEFT_48_PENALTY=1.185045 PRIMER_RIGHT_48_PENALTY=2.257154 PRIMER_LEFT_48_SEQUENCE=GGACCAAAACCTGCATTGATA PRIMER_RIGHT_48_SEQUENCE=AGTAAACTTTGGGACATGGCG PRIMER_LEFT_48=372,21 PRIMER_RIGHT_48=1248,21 PRIMER_LEFT_48_TM=59.815 PRIMER_RIGHT_48_TM=61.257 PRIMER_LEFT_48_GC_PERCENT=42.857 PRIMER_RIGHT_48_GC_PERCENT=47.619 PRIMER_LEFT_48_SELF_ANY=4.00 PRIMER_RIGHT_48_SELF_ANY=5.00 PRIMER_LEFT_48_SELF_END=2.00 PRIMER_RIGHT_48_SELF_END=2.00 PRIMER_LEFT_48_END_STABILITY=5.9000 PRIMER_RIGHT_48_END_STABILITY=11.7000 PRIMER_PAIR_48_COMPL_ANY=5.00 PRIMER_PAIR_48_COMPL_END=0.00 PRIMER_PAIR_48_PRODUCT_SIZE=877 PRIMER_PAIR_49_PENALTY=3.533320 PRIMER_LEFT_49_PENALTY=1.188029 PRIMER_RIGHT_49_PENALTY=2.345290 PRIMER_LEFT_49_SEQUENCE=AACCCCAGATTGCCATGTT PRIMER_RIGHT_49_SEQUENCE=CACACCTCTCGAACCACCT PRIMER_LEFT_49=291,19 PRIMER_RIGHT_49=1071,19 PRIMER_LEFT_49_TM=60.188 PRIMER_RIGHT_49_TM=58.655 PRIMER_LEFT_49_GC_PERCENT=47.368 PRIMER_RIGHT_49_GC_PERCENT=57.895 PRIMER_LEFT_49_SELF_ANY=4.00 PRIMER_RIGHT_49_SELF_ANY=4.00 PRIMER_LEFT_49_SELF_END=2.00 PRIMER_RIGHT_49_SELF_END=0.00 PRIMER_LEFT_49_END_STABILITY=6.6000 PRIMER_RIGHT_49_END_STABILITY=7.9000 PRIMER_PAIR_49_COMPL_ANY=3.00 PRIMER_PAIR_49_COMPL_END=1.00 PRIMER_PAIR_49_PRODUCT_SIZE=781 PRIMER_PAIR_50_PENALTY=3.541303 PRIMER_LEFT_50_PENALTY=2.402111 PRIMER_RIGHT_50_PENALTY=1.139192 PRIMER_LEFT_50_SEQUENCE=GGTTTGGCACTGCTCCTG PRIMER_RIGHT_50_SEQUENCE=TTCAGGGTAGACTTCTTGGCA PRIMER_LEFT_50=209,18 PRIMER_RIGHT_50=436,21 PRIMER_LEFT_50_TM=60.402 PRIMER_RIGHT_50_TM=59.861 PRIMER_LEFT_50_GC_PERCENT=61.111 PRIMER_RIGHT_50_GC_PERCENT=47.619 PRIMER_LEFT_50_SELF_ANY=5.00 PRIMER_RIGHT_50_SELF_ANY=4.00 PRIMER_LEFT_50_SELF_END=1.00 PRIMER_RIGHT_50_SELF_END=3.00 PRIMER_LEFT_50_END_STABILITY=8.2000 PRIMER_RIGHT_50_END_STABILITY=10.0000 PRIMER_PAIR_50_COMPL_ANY=4.00 PRIMER_PAIR_50_COMPL_END=2.00 PRIMER_PAIR_50_PRODUCT_SIZE=228 PRIMER_PAIR_51_PENALTY=3.591879 PRIMER_LEFT_51_PENALTY=1.236064 PRIMER_RIGHT_51_PENALTY=2.355815 PRIMER_LEFT_51_SEQUENCE=CCATCAGGGACCAAAACCT PRIMER_RIGHT_51_SEQUENCE=GTCTTCACTCCCATCTGCAT PRIMER_LEFT_51=365,19 PRIMER_RIGHT_51=868,20 PRIMER_LEFT_51_TM=59.764 PRIMER_RIGHT_51_TM=57.644 PRIMER_LEFT_51_GC_PERCENT=52.632 PRIMER_RIGHT_51_GC_PERCENT=50.000 PRIMER_LEFT_51_SELF_ANY=3.00 PRIMER_RIGHT_51_SELF_ANY=4.00 PRIMER_LEFT_51_SELF_END=0.00 PRIMER_RIGHT_51_SELF_END=2.00 PRIMER_LEFT_51_END_STABILITY=7.9000 PRIMER_RIGHT_51_END_STABILITY=8.4000 PRIMER_PAIR_51_COMPL_ANY=4.00 PRIMER_PAIR_51_COMPL_END=0.00 PRIMER_PAIR_51_PRODUCT_SIZE=504 PRIMER_PAIR_52_PENALTY=3.659168 PRIMER_LEFT_52_PENALTY=3.503107 PRIMER_RIGHT_52_PENALTY=0.156061 PRIMER_LEFT_52_SEQUENCE=GCTGGAGGTACCCACTGAT PRIMER_RIGHT_52_SEQUENCE=TTCCACCTCAGCCACTTCTT PRIMER_LEFT_52=250,19 PRIMER_RIGHT_52=913,20 PRIMER_LEFT_52_TM=57.497 PRIMER_RIGHT_52_TM=59.844 PRIMER_LEFT_52_GC_PERCENT=57.895 PRIMER_RIGHT_52_GC_PERCENT=50.000 PRIMER_LEFT_52_SELF_ANY=6.00 PRIMER_RIGHT_52_SELF_ANY=3.00 PRIMER_LEFT_52_SELF_END=2.00 PRIMER_RIGHT_52_SELF_END=0.00 PRIMER_LEFT_52_END_STABILITY=6.6000 PRIMER_RIGHT_52_END_STABILITY=7.0000 PRIMER_PAIR_52_COMPL_ANY=6.00 PRIMER_PAIR_52_COMPL_END=0.00 PRIMER_PAIR_52_PRODUCT_SIZE=664 PRIMER_PAIR_53_PENALTY=3.703097 PRIMER_LEFT_53_PENALTY=1.271611 PRIMER_RIGHT_53_PENALTY=2.431486 PRIMER_LEFT_53_SEQUENCE=GCCATGTTCTGTGGCAGAC PRIMER_RIGHT_53_SEQUENCE=TTCTCACTGCATGTCTCTTTGG PRIMER_LEFT_53=302,19 PRIMER_RIGHT_53=681,22 PRIMER_LEFT_53_TM=60.272 PRIMER_RIGHT_53_TM=60.431 PRIMER_LEFT_53_GC_PERCENT=57.895 PRIMER_RIGHT_53_GC_PERCENT=45.455 PRIMER_LEFT_53_SELF_ANY=5.00 PRIMER_RIGHT_53_SELF_ANY=4.00 PRIMER_LEFT_53_SELF_END=3.00 PRIMER_RIGHT_53_SELF_END=0.00 PRIMER_LEFT_53_END_STABILITY=6.4000 PRIMER_RIGHT_53_END_STABILITY=8.8000 PRIMER_PAIR_53_COMPL_ANY=4.00 PRIMER_PAIR_53_COMPL_END=1.00 PRIMER_PAIR_53_PRODUCT_SIZE=380 PRIMER_PAIR_54_PENALTY=3.733965 PRIMER_LEFT_54_PENALTY=3.598066 PRIMER_RIGHT_54_PENALTY=0.135900 PRIMER_LEFT_54_SEQUENCE=TATTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_54_SEQUENCE=CGAGATACTTGTCAACGGCA PRIMER_LEFT_54=413,22 PRIMER_RIGHT_54=1337,20 PRIMER_LEFT_54_TM=58.402 PRIMER_RIGHT_54_TM=59.864 PRIMER_LEFT_54_GC_PERCENT=45.455 PRIMER_RIGHT_54_GC_PERCENT=50.000 PRIMER_LEFT_54_SELF_ANY=5.00 PRIMER_RIGHT_54_SELF_ANY=5.00 PRIMER_LEFT_54_SELF_END=1.00 PRIMER_RIGHT_54_SELF_END=1.00 PRIMER_LEFT_54_END_STABILITY=9.7000 PRIMER_RIGHT_54_END_STABILITY=11.7000 PRIMER_PAIR_54_COMPL_ANY=7.00 PRIMER_PAIR_54_COMPL_END=2.00 PRIMER_PAIR_54_PRODUCT_SIZE=925 PRIMER_PAIR_55_PENALTY=3.734645 PRIMER_LEFT_55_PENALTY=2.495254 PRIMER_RIGHT_55_PENALTY=1.239392 PRIMER_LEFT_55_SEQUENCE=GTCAGTTTCCTCGGCAGC PRIMER_RIGHT_55_SEQUENCE=TACCATCAGTGGGTACCTCCA PRIMER_LEFT_55=50,18 PRIMER_RIGHT_55=272,21 PRIMER_LEFT_55_TM=59.505 PRIMER_RIGHT_55_TM=60.239 PRIMER_LEFT_55_GC_PERCENT=61.111 PRIMER_RIGHT_55_GC_PERCENT=52.381 PRIMER_LEFT_55_SELF_ANY=3.00 PRIMER_RIGHT_55_SELF_ANY=6.00 PRIMER_LEFT_55_SELF_END=3.00 PRIMER_RIGHT_55_SELF_END=2.00 PRIMER_LEFT_55_END_STABILITY=9.7000 PRIMER_RIGHT_55_END_STABILITY=8.2000 PRIMER_PAIR_55_COMPL_ANY=3.00 PRIMER_PAIR_55_COMPL_END=0.00 PRIMER_PAIR_55_PRODUCT_SIZE=223 PRIMER_PAIR_56_PENALTY=3.753608 PRIMER_LEFT_56_PENALTY=1.278124 PRIMER_RIGHT_56_PENALTY=2.475484 PRIMER_LEFT_56_SEQUENCE=CCAGAATGGGAAGTGGGAT PRIMER_RIGHT_56_SEQUENCE=GGCTGCTGTTGTAGGAAGTTT PRIMER_LEFT_56=340,19 PRIMER_RIGHT_56=1306,21 PRIMER_LEFT_56_TM=59.722 PRIMER_RIGHT_56_TM=58.525 PRIMER_LEFT_56_GC_PERCENT=52.632 PRIMER_RIGHT_56_GC_PERCENT=47.619 PRIMER_LEFT_56_SELF_ANY=3.00 PRIMER_RIGHT_56_SELF_ANY=3.00 PRIMER_LEFT_56_SELF_END=2.00 PRIMER_RIGHT_56_SELF_END=2.00 PRIMER_LEFT_56_END_STABILITY=9.3000 PRIMER_RIGHT_56_END_STABILITY=6.7000 PRIMER_PAIR_56_COMPL_ANY=3.00 PRIMER_PAIR_56_COMPL_END=0.00 PRIMER_PAIR_56_PRODUCT_SIZE=967 PRIMER_PAIR_57_PENALTY=3.795825 PRIMER_LEFT_57_PENALTY=1.292120 PRIMER_RIGHT_57_PENALTY=2.503705 PRIMER_LEFT_57_SEQUENCE=GCCATGTTCTGTGGCAGACT PRIMER_RIGHT_57_SEQUENCE=AAACTTTGGGACATGGCG PRIMER_LEFT_57=302,20 PRIMER_RIGHT_57=1245,18 PRIMER_LEFT_57_TM=61.292 PRIMER_RIGHT_57_TM=59.496 PRIMER_LEFT_57_GC_PERCENT=55.000 PRIMER_RIGHT_57_GC_PERCENT=50.000 PRIMER_LEFT_57_SELF_ANY=5.00 PRIMER_RIGHT_57_SELF_ANY=5.00 PRIMER_LEFT_57_SELF_END=3.00 PRIMER_RIGHT_57_SELF_END=2.00 PRIMER_LEFT_57_END_STABILITY=6.1000 PRIMER_RIGHT_57_END_STABILITY=11.7000 PRIMER_PAIR_57_COMPL_ANY=7.00 PRIMER_PAIR_57_COMPL_END=0.00 PRIMER_PAIR_57_PRODUCT_SIZE=944 PRIMER_PAIR_58_PENALTY=3.813730 PRIMER_LEFT_58_PENALTY=3.657669 PRIMER_RIGHT_58_PENALTY=0.156061 PRIMER_LEFT_58_SEQUENCE=GCAGTATTGCCAAGAAGTCTACC PRIMER_RIGHT_58_SEQUENCE=TTCTTCCACCTCAGCCACTT PRIMER_LEFT_58=409,23 PRIMER_RIGHT_58=916,20 PRIMER_LEFT_58_TM=59.342 PRIMER_RIGHT_58_TM=59.844 PRIMER_LEFT_58_GC_PERCENT=47.826 PRIMER_RIGHT_58_GC_PERCENT=50.000 PRIMER_LEFT_58_SELF_ANY=6.00 PRIMER_RIGHT_58_SELF_ANY=3.00 PRIMER_LEFT_58_SELF_END=1.00 PRIMER_RIGHT_58_SELF_END=0.00 PRIMER_LEFT_58_END_STABILITY=6.9000 PRIMER_RIGHT_58_END_STABILITY=6.7000 PRIMER_PAIR_58_COMPL_ANY=5.00 PRIMER_PAIR_58_COMPL_END=0.00 PRIMER_PAIR_58_PRODUCT_SIZE=508 PRIMER_PAIR_59_PENALTY=3.884306 PRIMER_LEFT_59_PENALTY=1.309943 PRIMER_RIGHT_59_PENALTY=2.574363 PRIMER_LEFT_59_SEQUENCE=GTCCAGAATGGGAAGTGGG PRIMER_RIGHT_59_SEQUENCE=TTGTTCAGAGCACACCTCTCG PRIMER_LEFT_59=338,19 PRIMER_RIGHT_59=1081,21 PRIMER_LEFT_59_TM=60.310 PRIMER_RIGHT_59_TM=61.574 PRIMER_LEFT_59_GC_PERCENT=57.895 PRIMER_RIGHT_59_GC_PERCENT=52.381 PRIMER_LEFT_59_SELF_ANY=3.00 PRIMER_RIGHT_59_SELF_ANY=5.00 PRIMER_LEFT_59_SELF_END=0.00 PRIMER_RIGHT_59_SELF_END=2.00 PRIMER_LEFT_59_END_STABILITY=9.4000 PRIMER_RIGHT_59_END_STABILITY=8.4000 PRIMER_PAIR_59_COMPL_ANY=4.00 PRIMER_PAIR_59_COMPL_END=3.00 PRIMER_PAIR_59_PRODUCT_SIZE=744 PRIMER_PAIR_60_PENALTY=3.967455 PRIMER_LEFT_60_PENALTY=1.312895 PRIMER_RIGHT_60_PENALTY=2.654560 PRIMER_LEFT_60_SEQUENCE=AGAATGGGAAGTGGGATTCAG PRIMER_RIGHT_60_SEQUENCE=GCACACCTCTCGAACCACCT PRIMER_LEFT_60=342,21 PRIMER_RIGHT_60=1072,20 PRIMER_LEFT_60_TM=60.313 PRIMER_RIGHT_60_TM=62.655 PRIMER_LEFT_60_GC_PERCENT=47.619 PRIMER_RIGHT_60_GC_PERCENT=60.000 PRIMER_LEFT_60_SELF_ANY=4.00 PRIMER_RIGHT_60_SELF_ANY=4.00 PRIMER_LEFT_60_SELF_END=1.00 PRIMER_RIGHT_60_SELF_END=0.00 PRIMER_LEFT_60_END_STABILITY=7.0000 PRIMER_RIGHT_60_END_STABILITY=7.9000 PRIMER_PAIR_60_COMPL_ANY=4.00 PRIMER_PAIR_60_COMPL_END=3.00 PRIMER_PAIR_60_PRODUCT_SIZE=731 PRIMER_PAIR_61_PENALTY=3.970758 PRIMER_LEFT_61_PENALTY=1.316198 PRIMER_RIGHT_61_PENALTY=2.654560 PRIMER_LEFT_61_SEQUENCE=CCCACTGATGGTAATGCTGG PRIMER_RIGHT_61_SEQUENCE=AGCACACCTCTCGAACCACC PRIMER_LEFT_61=260,20 PRIMER_RIGHT_61=1073,20 PRIMER_LEFT_61_TM=61.316 PRIMER_RIGHT_61_TM=62.655 PRIMER_LEFT_61_GC_PERCENT=55.000 PRIMER_RIGHT_61_GC_PERCENT=60.000 PRIMER_LEFT_61_SELF_ANY=3.00 PRIMER_RIGHT_61_SELF_ANY=4.00 PRIMER_LEFT_61_SELF_END=0.00 PRIMER_RIGHT_61_SELF_END=0.00 PRIMER_LEFT_61_END_STABILITY=9.7000 PRIMER_RIGHT_61_END_STABILITY=9.4000 PRIMER_PAIR_61_COMPL_ANY=4.00 PRIMER_PAIR_61_COMPL_END=3.00 PRIMER_PAIR_61_PRODUCT_SIZE=814 PRIMER_PAIR_62_PENALTY=3.989386 PRIMER_LEFT_62_PENALTY=3.791133 PRIMER_RIGHT_62_PENALTY=0.198253 PRIMER_LEFT_62_SEQUENCE=GTATTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_62_SEQUENCE=CAGACTCTGTGGTGGTGGTG PRIMER_LEFT_62=412,23 PRIMER_RIGHT_62=1046,20 PRIMER_LEFT_62_TM=59.209 PRIMER_RIGHT_62_TM=60.198 PRIMER_LEFT_62_GC_PERCENT=47.826 PRIMER_RIGHT_62_GC_PERCENT=60.000 PRIMER_LEFT_62_SELF_ANY=5.00 PRIMER_RIGHT_62_SELF_ANY=7.00 PRIMER_LEFT_62_SELF_END=1.00 PRIMER_RIGHT_62_SELF_END=0.00 PRIMER_LEFT_62_END_STABILITY=9.7000 PRIMER_RIGHT_62_END_STABILITY=8.2000 PRIMER_PAIR_62_COMPL_ANY=6.00 PRIMER_PAIR_62_COMPL_END=0.00 PRIMER_PAIR_62_PRODUCT_SIZE=635 PRIMER_PAIR_63_PENALTY=3.996838 PRIMER_LEFT_63_PENALTY=3.791133 PRIMER_RIGHT_63_PENALTY=0.205704 PRIMER_LEFT_63_SEQUENCE=CAGTATTGCCAAGAAGTCTACCC PRIMER_RIGHT_63_SEQUENCE=TTAACAGGATCTCGGGCAAG PRIMER_LEFT_63=410,23 PRIMER_RIGHT_63=1287,20 PRIMER_LEFT_63_TM=59.209 PRIMER_RIGHT_63_TM=60.206 PRIMER_LEFT_63_GC_PERCENT=47.826 PRIMER_RIGHT_63_GC_PERCENT=50.000 PRIMER_LEFT_63_SELF_ANY=5.00 PRIMER_RIGHT_63_SELF_ANY=4.00 PRIMER_LEFT_63_SELF_END=0.00 PRIMER_RIGHT_63_SELF_END=2.00 PRIMER_LEFT_63_END_STABILITY=8.4000 PRIMER_RIGHT_63_END_STABILITY=8.5000 PRIMER_PAIR_63_COMPL_ANY=5.00 PRIMER_PAIR_63_COMPL_END=2.00 PRIMER_PAIR_63_PRODUCT_SIZE=878 PRIMER_PAIR_64_PENALTY=4.059135 PRIMER_LEFT_64_PENALTY=2.500904 PRIMER_RIGHT_64_PENALTY=1.558232 PRIMER_LEFT_64_SEQUENCE=GCTCCGTCAGTTTCCTCG PRIMER_RIGHT_64_SEQUENCE=TCAGGGTAGACTTCTTGGCA PRIMER_LEFT_64=45,18 PRIMER_RIGHT_64=435,20 PRIMER_LEFT_64_TM=59.499 PRIMER_RIGHT_64_TM=58.442 PRIMER_LEFT_64_GC_PERCENT=61.111 PRIMER_RIGHT_64_GC_PERCENT=50.000 PRIMER_LEFT_64_SELF_ANY=2.00 PRIMER_RIGHT_64_SELF_ANY=4.00 PRIMER_LEFT_64_SELF_END=2.00 PRIMER_RIGHT_64_SELF_END=3.00 PRIMER_LEFT_64_END_STABILITY=9.9000 PRIMER_RIGHT_64_END_STABILITY=10.0000 PRIMER_PAIR_64_COMPL_ANY=3.00 PRIMER_PAIR_64_COMPL_END=1.00 PRIMER_PAIR_64_PRODUCT_SIZE=391 PRIMER_PAIR_65_PENALTY=4.064642 PRIMER_LEFT_65_PENALTY=1.392601 PRIMER_RIGHT_65_PENALTY=2.672041 PRIMER_LEFT_65_SEQUENCE=TCCTGCAGTATTGCCAAGAAG PRIMER_RIGHT_65_SEQUENCE=CACACCTCTCGAACCACCTCT PRIMER_LEFT_65=405,21 PRIMER_RIGHT_65=1071,21 PRIMER_LEFT_65_TM=60.393 PRIMER_RIGHT_65_TM=61.672 PRIMER_LEFT_65_GC_PERCENT=47.619 PRIMER_RIGHT_65_GC_PERCENT=57.143 PRIMER_LEFT_65_SELF_ANY=6.00 PRIMER_RIGHT_65_SELF_ANY=4.00 PRIMER_LEFT_65_SELF_END=1.00 PRIMER_RIGHT_65_SELF_END=0.00 PRIMER_LEFT_65_END_STABILITY=6.7000 PRIMER_RIGHT_65_END_STABILITY=7.9000 PRIMER_PAIR_65_COMPL_ANY=3.00 PRIMER_PAIR_65_COMPL_END=3.00 PRIMER_PAIR_65_PRODUCT_SIZE=667 PRIMER_PAIR_66_PENALTY=4.067344 PRIMER_LEFT_66_PENALTY=1.357294 PRIMER_RIGHT_66_PENALTY=2.710050 PRIMER_LEFT_66_SEQUENCE=AATGGGAAGTGGGATTCAGAT PRIMER_RIGHT_66_SEQUENCE=AACTCACCAACTAAGCAGCGG PRIMER_LEFT_66=344,21 PRIMER_RIGHT_66=564,21 PRIMER_LEFT_66_TM=59.643 PRIMER_RIGHT_66_TM=61.710 PRIMER_LEFT_66_GC_PERCENT=42.857 PRIMER_RIGHT_66_GC_PERCENT=52.381 PRIMER_LEFT_66_SELF_ANY=3.00 PRIMER_RIGHT_66_SELF_ANY=3.00 PRIMER_LEFT_66_SELF_END=2.00 PRIMER_RIGHT_66_SELF_END=2.00 PRIMER_LEFT_66_END_STABILITY=6.6000 PRIMER_RIGHT_66_END_STABILITY=11.4000 PRIMER_PAIR_66_COMPL_ANY=4.00 PRIMER_PAIR_66_COMPL_END=1.00 PRIMER_PAIR_66_PRODUCT_SIZE=221 PRIMER_PAIR_67_PENALTY=4.156276 PRIMER_LEFT_67_PENALTY=1.427808 PRIMER_RIGHT_67_PENALTY=2.728469 PRIMER_LEFT_67_SEQUENCE=CATTGATACCAAGGAAGGCA PRIMER_RIGHT_67_SEQUENCE=GCACACCTCTCGAACCACC PRIMER_LEFT_67=385,20 PRIMER_RIGHT_67=1072,19 PRIMER_LEFT_67_TM=58.572 PRIMER_RIGHT_67_TM=61.728 PRIMER_LEFT_67_GC_PERCENT=45.000 PRIMER_RIGHT_67_GC_PERCENT=63.158 PRIMER_LEFT_67_SELF_ANY=6.00 PRIMER_RIGHT_67_SELF_ANY=4.00 PRIMER_LEFT_67_SELF_END=0.00 PRIMER_RIGHT_67_SELF_END=0.00 PRIMER_LEFT_67_END_STABILITY=9.7000 PRIMER_RIGHT_67_END_STABILITY=9.4000 PRIMER_PAIR_67_COMPL_ANY=3.00 PRIMER_PAIR_67_COMPL_END=0.00 PRIMER_PAIR_67_PRODUCT_SIZE=688 PRIMER_PAIR_68_PENALTY=4.222413 PRIMER_LEFT_68_PENALTY=2.546814 PRIMER_RIGHT_68_PENALTY=1.675600 PRIMER_LEFT_68_SEQUENCE=CGTCAGTTTCCTCGGCAG PRIMER_RIGHT_68_SEQUENCE=CCATCAGTGGGTACCTCCA PRIMER_LEFT_68=49,18 PRIMER_RIGHT_68=270,19 PRIMER_LEFT_68_TM=60.547 PRIMER_RIGHT_68_TM=59.324 PRIMER_LEFT_68_GC_PERCENT=61.111 PRIMER_RIGHT_68_GC_PERCENT=57.895 PRIMER_LEFT_68_SELF_ANY=2.00 PRIMER_RIGHT_68_SELF_ANY=6.00 PRIMER_LEFT_68_SELF_END=2.00 PRIMER_RIGHT_68_SELF_END=2.00 PRIMER_LEFT_68_END_STABILITY=9.7000 PRIMER_RIGHT_68_END_STABILITY=8.2000 PRIMER_PAIR_68_COMPL_ANY=3.00 PRIMER_PAIR_68_COMPL_END=1.00 PRIMER_PAIR_68_PRODUCT_SIZE=222 PRIMER_PAIR_69_PENALTY=4.245946 PRIMER_LEFT_69_PENALTY=4.008330 PRIMER_RIGHT_69_PENALTY=0.237616 PRIMER_LEFT_69_SEQUENCE=CCAAGAAGTCTACCCTGAACTG PRIMER_RIGHT_69_SEQUENCE=TCTCGAACCACCTCTTCCAC PRIMER_LEFT_69=418,22 PRIMER_RIGHT_69=1065,20 PRIMER_LEFT_69_TM=57.992 PRIMER_RIGHT_69_TM=60.238 PRIMER_LEFT_69_GC_PERCENT=50.000 PRIMER_RIGHT_69_GC_PERCENT=55.000 PRIMER_LEFT_69_SELF_ANY=4.00 PRIMER_RIGHT_69_SELF_ANY=4.00 PRIMER_LEFT_69_SELF_END=1.00 PRIMER_RIGHT_69_SELF_END=0.00 PRIMER_LEFT_69_END_STABILITY=6.7000 PRIMER_RIGHT_69_END_STABILITY=7.9000 PRIMER_PAIR_69_COMPL_ANY=5.00 PRIMER_PAIR_69_COMPL_END=1.00 PRIMER_PAIR_69_PRODUCT_SIZE=648 PRIMER_PAIR_70_PENALTY=4.251723 PRIMER_LEFT_70_PENALTY=1.427808 PRIMER_RIGHT_70_PENALTY=2.823915 PRIMER_LEFT_70_SEQUENCE=TGCATTGATACCAAGGAAGG PRIMER_RIGHT_70_SEQUENCE=AGAGCACACCTCTCGAACCAC PRIMER_LEFT_70=383,20 PRIMER_RIGHT_70=1075,21 PRIMER_LEFT_70_TM=58.572 PRIMER_RIGHT_70_TM=61.824 PRIMER_LEFT_70_GC_PERCENT=45.000 PRIMER_RIGHT_70_GC_PERCENT=57.143 PRIMER_LEFT_70_SELF_ANY=6.00 PRIMER_RIGHT_70_SELF_ANY=4.00 PRIMER_LEFT_70_SELF_END=0.00 PRIMER_RIGHT_70_SELF_END=0.00 PRIMER_LEFT_70_END_STABILITY=8.2000 PRIMER_RIGHT_70_END_STABILITY=7.6000 PRIMER_PAIR_70_COMPL_ANY=3.00 PRIMER_PAIR_70_COMPL_END=1.00 PRIMER_PAIR_70_PRODUCT_SIZE=693 PRIMER_PAIR_71_PENALTY=4.316825 PRIMER_LEFT_71_PENALTY=1.440351 PRIMER_RIGHT_71_PENALTY=2.876473 PRIMER_LEFT_71_SEQUENCE=TCAGATCCATCAGGGACCAA PRIMER_RIGHT_71_SEQUENCE=TCAGAGCACACCTCTCGAA PRIMER_LEFT_71=359,20 PRIMER_RIGHT_71=1077,19 PRIMER_LEFT_71_TM=61.440 PRIMER_RIGHT_71_TM=58.124 PRIMER_LEFT_71_GC_PERCENT=50.000 PRIMER_RIGHT_71_GC_PERCENT=52.632 PRIMER_LEFT_71_SELF_ANY=5.00 PRIMER_RIGHT_71_SELF_ANY=4.00 PRIMER_LEFT_71_SELF_END=1.00 PRIMER_RIGHT_71_SELF_END=2.00 PRIMER_LEFT_71_END_STABILITY=8.2000 PRIMER_RIGHT_71_END_STABILITY=8.7000 PRIMER_PAIR_71_COMPL_ANY=3.00 PRIMER_PAIR_71_COMPL_END=0.00 PRIMER_PAIR_71_PRODUCT_SIZE=719 PRIMER_PAIR_72_PENALTY=4.329868 PRIMER_LEFT_72_PENALTY=4.076046 PRIMER_RIGHT_72_PENALTY=0.253823 PRIMER_LEFT_72_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_72_SEQUENCE=CATGCCGTAGTCATGCAAGT PRIMER_LEFT_72=411,24 PRIMER_RIGHT_72=709,20 PRIMER_LEFT_72_TM=60.076 PRIMER_RIGHT_72_TM=59.746 PRIMER_LEFT_72_GC_PERCENT=45.833 PRIMER_RIGHT_72_GC_PERCENT=50.000 PRIMER_LEFT_72_SELF_ANY=5.00 PRIMER_RIGHT_72_SELF_ANY=6.00 PRIMER_LEFT_72_SELF_END=1.00 PRIMER_RIGHT_72_SELF_END=2.00 PRIMER_LEFT_72_END_STABILITY=9.7000 PRIMER_RIGHT_72_END_STABILITY=6.7000 PRIMER_PAIR_72_COMPL_ANY=4.00 PRIMER_PAIR_72_COMPL_END=1.00 PRIMER_PAIR_72_PRODUCT_SIZE=299 PRIMER_PAIR_73_PENALTY=4.360420 PRIMER_LEFT_73_PENALTY=4.076046 PRIMER_RIGHT_73_PENALTY=0.284374 PRIMER_LEFT_73_SEQUENCE=CAGTATTGCCAAGAAGTCTACCCT PRIMER_RIGHT_73_SEQUENCE=TCACATCAAAGTACCAGCGG PRIMER_LEFT_73=410,24 PRIMER_RIGHT_73=1133,20 PRIMER_LEFT_73_TM=60.076 PRIMER_RIGHT_73_TM=59.716 PRIMER_LEFT_73_GC_PERCENT=45.833 PRIMER_RIGHT_73_GC_PERCENT=50.000 PRIMER_LEFT_73_SELF_ANY=5.00 PRIMER_RIGHT_73_SELF_ANY=4.00 PRIMER_LEFT_73_SELF_END=2.00 PRIMER_RIGHT_73_SELF_END=2.00 PRIMER_LEFT_73_END_STABILITY=9.1000 PRIMER_RIGHT_73_END_STABILITY=11.4000 PRIMER_PAIR_73_COMPL_ANY=3.00 PRIMER_PAIR_73_COMPL_END=2.00 PRIMER_PAIR_73_PRODUCT_SIZE=724 PRIMER_PAIR_74_PENALTY=4.383084 PRIMER_LEFT_74_PENALTY=1.440351 PRIMER_RIGHT_74_PENALTY=2.942733 PRIMER_LEFT_74_SEQUENCE=TTCAGATCCATCAGGGACCA PRIMER_RIGHT_74_SEQUENCE=CTGCATGTCTCTTTGGCG PRIMER_LEFT_74=358,20 PRIMER_RIGHT_74=675,18 PRIMER_LEFT_74_TM=61.440 PRIMER_RIGHT_74_TM=59.057 PRIMER_LEFT_74_GC_PERCENT=50.000 PRIMER_RIGHT_74_GC_PERCENT=55.556 PRIMER_LEFT_74_SELF_ANY=5.00 PRIMER_RIGHT_74_SELF_ANY=4.00 PRIMER_LEFT_74_SELF_END=3.00 PRIMER_RIGHT_74_SELF_END=2.00 PRIMER_LEFT_74_END_STABILITY=7.9000 PRIMER_RIGHT_74_END_STABILITY=11.7000 PRIMER_PAIR_74_COMPL_ANY=5.00 PRIMER_PAIR_74_COMPL_END=1.00 PRIMER_PAIR_74_PRODUCT_SIZE=318 PRIMER_PAIR_75_PENALTY=4.416017 PRIMER_LEFT_75_PENALTY=1.468408 PRIMER_RIGHT_75_PENALTY=2.947608 PRIMER_LEFT_75_SEQUENCE=CCACTGATGGTAATGCTGGC PRIMER_RIGHT_75_SEQUENCE=CACTGCATGTCTCTTTGGCG PRIMER_LEFT_75=261,20 PRIMER_RIGHT_75=677,20 PRIMER_LEFT_75_TM=61.468 PRIMER_RIGHT_75_TM=62.948 PRIMER_LEFT_75_GC_PERCENT=55.000 PRIMER_RIGHT_75_GC_PERCENT=55.000 PRIMER_LEFT_75_SELF_ANY=3.00 PRIMER_RIGHT_75_SELF_ANY=4.00 PRIMER_LEFT_75_SELF_END=2.00 PRIMER_RIGHT_75_SELF_END=2.00 PRIMER_LEFT_75_END_STABILITY=9.7000 PRIMER_RIGHT_75_END_STABILITY=11.7000 PRIMER_PAIR_75_COMPL_ANY=4.00 PRIMER_PAIR_75_COMPL_END=1.00 PRIMER_PAIR_75_PRODUCT_SIZE=417 PRIMER_PAIR_76_PENALTY=4.519707 PRIMER_LEFT_76_PENALTY=4.253992 PRIMER_RIGHT_76_PENALTY=0.265715 PRIMER_LEFT_76_SEQUENCE=TGCAGTATTGCCAAGAAGTCTA PRIMER_RIGHT_76_SEQUENCE=TGTGGCTTCTTCGTAGGGTT PRIMER_LEFT_76=408,22 PRIMER_RIGHT_76=997,20 PRIMER_LEFT_76_TM=57.746 PRIMER_RIGHT_76_TM=59.734 PRIMER_LEFT_76_GC_PERCENT=40.909 PRIMER_RIGHT_76_GC_PERCENT=50.000 PRIMER_LEFT_76_SELF_ANY=6.00 PRIMER_RIGHT_76_SELF_ANY=4.00 PRIMER_LEFT_76_SELF_END=2.00 PRIMER_RIGHT_76_SELF_END=0.00 PRIMER_LEFT_76_END_STABILITY=5.4000 PRIMER_RIGHT_76_END_STABILITY=9.4000 PRIMER_PAIR_76_COMPL_ANY=6.00 PRIMER_PAIR_76_COMPL_END=3.00 PRIMER_PAIR_76_PRODUCT_SIZE=590 PRIMER_PAIR_77_PENALTY=4.540067 PRIMER_LEFT_77_PENALTY=1.472425 PRIMER_RIGHT_77_PENALTY=3.067642 PRIMER_LEFT_77_SEQUENCE=CCTGCAGTATTGCCAAGAAG PRIMER_RIGHT_77_SEQUENCE=AGAGCACACCTCTCGAACC PRIMER_LEFT_77=406,20 PRIMER_RIGHT_77=1075,19 PRIMER_LEFT_77_TM=58.528 PRIMER_RIGHT_77_TM=57.932 PRIMER_LEFT_77_GC_PERCENT=50.000 PRIMER_RIGHT_77_GC_PERCENT=57.895 PRIMER_LEFT_77_SELF_ANY=6.00 PRIMER_RIGHT_77_SELF_ANY=4.00 PRIMER_LEFT_77_SELF_END=1.00 PRIMER_RIGHT_77_SELF_END=0.00 PRIMER_LEFT_77_END_STABILITY=6.7000 PRIMER_RIGHT_77_END_STABILITY=7.9000 PRIMER_PAIR_77_COMPL_ANY=3.00 PRIMER_PAIR_77_COMPL_END=1.00 PRIMER_PAIR_77_PRODUCT_SIZE=670 PRIMER_PAIR_78_PENALTY=4.550427 PRIMER_LEFT_78_PENALTY=4.266053 PRIMER_RIGHT_78_PENALTY=0.284374 PRIMER_LEFT_78_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTA PRIMER_RIGHT_78_SEQUENCE=TACTTGTCAACGGCATCAGG PRIMER_LEFT_78=407,23 PRIMER_RIGHT_78=1332,20 PRIMER_LEFT_78_TM=58.734 PRIMER_RIGHT_78_TM=59.716 PRIMER_LEFT_78_GC_PERCENT=43.478 PRIMER_RIGHT_78_GC_PERCENT=50.000 PRIMER_LEFT_78_SELF_ANY=6.00 PRIMER_RIGHT_78_SELF_ANY=5.00 PRIMER_LEFT_78_SELF_END=2.00 PRIMER_RIGHT_78_SELF_END=1.00 PRIMER_LEFT_78_END_STABILITY=5.4000 PRIMER_RIGHT_78_END_STABILITY=8.2000 PRIMER_PAIR_78_COMPL_ANY=6.00 PRIMER_PAIR_78_COMPL_END=2.00 PRIMER_PAIR_78_PRODUCT_SIZE=926 PRIMER_PAIR_79_PENALTY=4.611847 PRIMER_LEFT_79_PENALTY=4.326727 PRIMER_RIGHT_79_PENALTY=0.285121 PRIMER_LEFT_79_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_79_SEQUENCE=ACGGCCATGCAGTACTCTTC PRIMER_LEFT_79=414,23 PRIMER_RIGHT_79=1218,20 PRIMER_LEFT_79_TM=61.327 PRIMER_RIGHT_79_TM=60.285 PRIMER_LEFT_79_GC_PERCENT=43.478 PRIMER_RIGHT_79_GC_PERCENT=55.000 PRIMER_LEFT_79_SELF_ANY=5.00 PRIMER_RIGHT_79_SELF_ANY=6.00 PRIMER_LEFT_79_SELF_END=0.00 PRIMER_RIGHT_79_SELF_END=2.00 PRIMER_LEFT_79_END_STABILITY=7.0000 PRIMER_RIGHT_79_END_STABILITY=6.7000 PRIMER_PAIR_79_COMPL_ANY=5.00 PRIMER_PAIR_79_COMPL_END=3.00 PRIMER_PAIR_79_PRODUCT_SIZE=805 PRIMER_PAIR_80_PENALTY=4.614912 PRIMER_LEFT_80_PENALTY=1.512710 PRIMER_RIGHT_80_PENALTY=3.102202 PRIMER_LEFT_80_SEQUENCE=TGAATGTCCAGAATGGGAAG PRIMER_RIGHT_80_SEQUENCE=CACTGCATGTCTCTTTGGC PRIMER_LEFT_80=333,20 PRIMER_RIGHT_80=677,19 PRIMER_LEFT_80_TM=58.487 PRIMER_RIGHT_80_TM=57.898 PRIMER_LEFT_80_GC_PERCENT=45.000 PRIMER_RIGHT_80_GC_PERCENT=52.632 PRIMER_LEFT_80_SELF_ANY=3.00 PRIMER_RIGHT_80_SELF_ANY=4.00 PRIMER_LEFT_80_SELF_END=0.00 PRIMER_RIGHT_80_SELF_END=2.00 PRIMER_LEFT_80_END_STABILITY=8.2000 PRIMER_RIGHT_80_END_STABILITY=10.0000 PRIMER_PAIR_80_COMPL_ANY=4.00 PRIMER_PAIR_80_COMPL_END=1.00 PRIMER_PAIR_80_PRODUCT_SIZE=345 PRIMER_PAIR_81_PENALTY=4.689041 PRIMER_LEFT_81_PENALTY=4.399603 PRIMER_RIGHT_81_PENALTY=0.289438 PRIMER_LEFT_81_SEQUENCE=TGCAGTATTGCCAAGAAGTCTAC PRIMER_RIGHT_81_SEQUENCE=GCATGTTCATTCTCATCCCC PRIMER_LEFT_81=408,23 PRIMER_RIGHT_81=1365,20 PRIMER_LEFT_81_TM=58.600 PRIMER_RIGHT_81_TM=60.289 PRIMER_LEFT_81_GC_PERCENT=43.478 PRIMER_RIGHT_81_GC_PERCENT=50.000 PRIMER_LEFT_81_SELF_ANY=6.00 PRIMER_RIGHT_81_SELF_ANY=4.00 PRIMER_LEFT_81_SELF_END=3.00 PRIMER_RIGHT_81_SELF_END=0.00 PRIMER_LEFT_81_END_STABILITY=5.4000 PRIMER_RIGHT_81_END_STABILITY=10.9000 PRIMER_PAIR_81_COMPL_ANY=4.00 PRIMER_PAIR_81_COMPL_END=0.00 PRIMER_PAIR_81_PRODUCT_SIZE=958 PRIMER_PAIR_82_PENALTY=4.693533 PRIMER_LEFT_82_PENALTY=2.563176 PRIMER_RIGHT_82_PENALTY=2.130358 PRIMER_LEFT_82_SEQUENCE=GTTTGGCACTGCTCCTGCT PRIMER_RIGHT_82_SEQUENCE=TCAGGGTAGACTTCTTGGCAAT PRIMER_LEFT_82=210,19 PRIMER_RIGHT_82=435,22 PRIMER_LEFT_82_TM=61.563 PRIMER_RIGHT_82_TM=60.130 PRIMER_LEFT_82_GC_PERCENT=57.895 PRIMER_RIGHT_82_GC_PERCENT=45.455 PRIMER_LEFT_82_SELF_ANY=5.00 PRIMER_RIGHT_82_SELF_ANY=5.00 PRIMER_LEFT_82_SELF_END=0.00 PRIMER_RIGHT_82_SELF_END=3.00 PRIMER_LEFT_82_END_STABILITY=8.2000 PRIMER_RIGHT_82_END_STABILITY=8.4000 PRIMER_PAIR_82_COMPL_ANY=4.00 PRIMER_PAIR_82_COMPL_END=0.00 PRIMER_PAIR_82_PRODUCT_SIZE=226 PRIMER_PAIR_83_PENALTY=4.693889 PRIMER_LEFT_83_PENALTY=1.513670 PRIMER_RIGHT_83_PENALTY=3.180219 PRIMER_LEFT_83_SEQUENCE=GGACCAAAACCTGCATTGA PRIMER_RIGHT_83_SEQUENCE=CTTTGTCTTCACTCCCATCTGC PRIMER_LEFT_83=372,19 PRIMER_RIGHT_83=872,22 PRIMER_LEFT_83_TM=59.486 PRIMER_RIGHT_83_TM=61.180 PRIMER_LEFT_83_GC_PERCENT=47.368 PRIMER_RIGHT_83_GC_PERCENT=50.000 PRIMER_LEFT_83_SELF_ANY=4.00 PRIMER_RIGHT_83_SELF_ANY=2.00 PRIMER_LEFT_83_SELF_END=1.00 PRIMER_RIGHT_83_SELF_END=2.00 PRIMER_LEFT_83_END_STABILITY=6.9000 PRIMER_RIGHT_83_END_STABILITY=8.2000 PRIMER_PAIR_83_COMPL_ANY=4.00 PRIMER_PAIR_83_COMPL_END=1.00 PRIMER_PAIR_83_PRODUCT_SIZE=501 PRIMER_PAIR_84_PENALTY=4.715844 PRIMER_LEFT_84_PENALTY=1.532916 PRIMER_RIGHT_84_PENALTY=3.182928 PRIMER_LEFT_84_SEQUENCE=TGCACATGAATGTCCAGAATG PRIMER_RIGHT_84_SEQUENCE=GTCTTCACTCCCATCTGCATAG PRIMER_LEFT_84=327,21 PRIMER_RIGHT_84=868,22 PRIMER_LEFT_84_TM=60.533 PRIMER_RIGHT_84_TM=58.817 PRIMER_LEFT_84_GC_PERCENT=42.857 PRIMER_RIGHT_84_GC_PERCENT=50.000 PRIMER_LEFT_84_SELF_ANY=8.00 PRIMER_RIGHT_84_SELF_ANY=4.00 PRIMER_LEFT_84_SELF_END=1.00 PRIMER_RIGHT_84_SELF_END=2.00 PRIMER_LEFT_84_END_STABILITY=6.9000 PRIMER_RIGHT_84_END_STABILITY=5.9000 PRIMER_PAIR_84_COMPL_ANY=6.00 PRIMER_PAIR_84_COMPL_END=3.00 PRIMER_PAIR_84_PRODUCT_SIZE=542 PRIMER_PAIR_85_PENALTY=4.720614 PRIMER_LEFT_85_PENALTY=1.532916 PRIMER_RIGHT_85_PENALTY=3.187697 PRIMER_LEFT_85_SEQUENCE=CATGCACATGAATGTCCAGAA PRIMER_RIGHT_85_SEQUENCE=TGGCTGCTGTTGTAGGAAGTTT PRIMER_LEFT_85=325,21 PRIMER_RIGHT_85=1307,22 PRIMER_LEFT_85_TM=60.533 PRIMER_RIGHT_85_TM=61.188 PRIMER_LEFT_85_GC_PERCENT=42.857 PRIMER_RIGHT_85_GC_PERCENT=45.455 PRIMER_LEFT_85_SELF_ANY=8.00 PRIMER_RIGHT_85_SELF_ANY=3.00 PRIMER_LEFT_85_SELF_END=3.00 PRIMER_RIGHT_85_SELF_END=2.00 PRIMER_LEFT_85_END_STABILITY=7.0000 PRIMER_RIGHT_85_END_STABILITY=6.7000 PRIMER_PAIR_85_COMPL_ANY=3.00 PRIMER_PAIR_85_COMPL_END=2.00 PRIMER_PAIR_85_PRODUCT_SIZE=983 PRIMER_PAIR_86_PENALTY=4.748900 PRIMER_LEFT_86_PENALTY=1.543091 PRIMER_RIGHT_86_PENALTY=3.205809 PRIMER_LEFT_86_SEQUENCE=TGTCCAGAATGGGAAGTGG PRIMER_RIGHT_86_SEQUENCE=ACTCACCAACTAAGCAGCGGTA PRIMER_LEFT_86=337,19 PRIMER_RIGHT_86=563,22 PRIMER_LEFT_86_TM=59.457 PRIMER_RIGHT_86_TM=61.206 PRIMER_LEFT_86_GC_PERCENT=52.632 PRIMER_RIGHT_86_GC_PERCENT=50.000 PRIMER_LEFT_86_SELF_ANY=3.00 PRIMER_RIGHT_86_SELF_ANY=3.00 PRIMER_LEFT_86_SELF_END=0.00 PRIMER_RIGHT_86_SELF_END=2.00 PRIMER_LEFT_86_END_STABILITY=7.9000 PRIMER_RIGHT_86_END_STABILITY=8.9000 PRIMER_PAIR_86_COMPL_ANY=4.00 PRIMER_PAIR_86_COMPL_END=0.00 PRIMER_PAIR_86_PRODUCT_SIZE=227 PRIMER_PAIR_87_PENALTY=4.772082 PRIMER_LEFT_87_PENALTY=4.482644 PRIMER_RIGHT_87_PENALTY=0.289438 PRIMER_LEFT_87_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTAC PRIMER_RIGHT_87_SEQUENCE=GGGCATGTTCATTCTCATCC PRIMER_LEFT_87=407,24 PRIMER_RIGHT_87=1367,20 PRIMER_LEFT_87_TM=59.517 PRIMER_RIGHT_87_TM=60.289 PRIMER_LEFT_87_GC_PERCENT=45.833 PRIMER_RIGHT_87_GC_PERCENT=50.000 PRIMER_LEFT_87_SELF_ANY=6.00 PRIMER_RIGHT_87_SELF_ANY=4.00 PRIMER_LEFT_87_SELF_END=3.00 PRIMER_RIGHT_87_SELF_END=2.00 PRIMER_LEFT_87_END_STABILITY=5.4000 PRIMER_RIGHT_87_END_STABILITY=8.1000 PRIMER_PAIR_87_COMPL_ANY=4.00 PRIMER_PAIR_87_COMPL_END=0.00 PRIMER_PAIR_87_PRODUCT_SIZE=961 PRIMER_PAIR_88_PENALTY=4.801409 PRIMER_LEFT_88_PENALTY=4.514227 PRIMER_RIGHT_88_PENALTY=0.287182 PRIMER_LEFT_88_SEQUENCE=CCAAGAAGTCTACCCTGAACTGC PRIMER_RIGHT_88_SEQUENCE=GGCTTCTTCGTAGGGTTCCT PRIMER_LEFT_88=418,23 PRIMER_RIGHT_88=994,20 PRIMER_LEFT_88_TM=61.514 PRIMER_RIGHT_88_TM=59.713 PRIMER_LEFT_88_GC_PERCENT=52.174 PRIMER_RIGHT_88_GC_PERCENT=55.000 PRIMER_LEFT_88_SELF_ANY=4.00 PRIMER_RIGHT_88_SELF_ANY=4.00 PRIMER_LEFT_88_SELF_END=2.00 PRIMER_RIGHT_88_SELF_END=3.00 PRIMER_LEFT_88_END_STABILITY=7.9000 PRIMER_RIGHT_88_END_STABILITY=8.2000 PRIMER_PAIR_88_COMPL_ANY=6.00 PRIMER_PAIR_88_COMPL_END=3.00 PRIMER_PAIR_88_PRODUCT_SIZE=577 PRIMER_PAIR_89_PENALTY=4.820376 PRIMER_LEFT_89_PENALTY=4.514227 PRIMER_RIGHT_89_PENALTY=0.306150 PRIMER_LEFT_89_SEQUENCE=GCCAAGAAGTCTACCCTGAACTG PRIMER_RIGHT_89_SEQUENCE=AAAGTACCAGCGGGAGATCA PRIMER_LEFT_89=417,23 PRIMER_RIGHT_89=1126,20 PRIMER_LEFT_89_TM=61.514 PRIMER_RIGHT_89_TM=59.694 PRIMER_LEFT_89_GC_PERCENT=52.174 PRIMER_RIGHT_89_GC_PERCENT=50.000 PRIMER_LEFT_89_SELF_ANY=4.00 PRIMER_RIGHT_89_SELF_ANY=4.00 PRIMER_LEFT_89_SELF_END=1.00 PRIMER_RIGHT_89_SELF_END=2.00 PRIMER_LEFT_89_END_STABILITY=6.7000 PRIMER_RIGHT_89_END_STABILITY=6.6000 PRIMER_PAIR_89_COMPL_ANY=5.00 PRIMER_PAIR_89_COMPL_END=3.00 PRIMER_PAIR_89_PRODUCT_SIZE=710 PRIMER_PAIR_90_PENALTY=4.834837 PRIMER_LEFT_90_PENALTY=4.514227 PRIMER_RIGHT_90_PENALTY=0.320610 PRIMER_LEFT_90_SEQUENCE=TGCCAAGAAGTCTACCCTGAACT PRIMER_RIGHT_90_SEQUENCE=CTTTGGCTTTCTGGAAATGG PRIMER_LEFT_90=416,23 PRIMER_RIGHT_90=1385,20 PRIMER_LEFT_90_TM=61.514 PRIMER_RIGHT_90_TM=59.679 PRIMER_LEFT_90_GC_PERCENT=47.826 PRIMER_RIGHT_90_GC_PERCENT=45.000 PRIMER_LEFT_90_SELF_ANY=4.00 PRIMER_RIGHT_90_SELF_ANY=6.00 PRIMER_LEFT_90_SELF_END=1.00 PRIMER_RIGHT_90_SELF_END=2.00 PRIMER_LEFT_90_END_STABILITY=6.4000 PRIMER_RIGHT_90_END_STABILITY=8.4000 PRIMER_PAIR_90_COMPL_ANY=5.00 PRIMER_PAIR_90_COMPL_END=0.00 PRIMER_PAIR_90_PRODUCT_SIZE=970 PRIMER_PAIR_91_PENALTY=4.877959 PRIMER_LEFT_91_PENALTY=1.557214 PRIMER_RIGHT_91_PENALTY=3.320745 PRIMER_LEFT_91_SEQUENCE=ACCCACTGATGGTAATGCTG PRIMER_RIGHT_91_SEQUENCE=CTCACCAACTAAGCAGCGGTAG PRIMER_LEFT_91=259,20 PRIMER_RIGHT_91=562,22 PRIMER_LEFT_91_TM=58.443 PRIMER_RIGHT_91_TM=61.321 PRIMER_LEFT_91_GC_PERCENT=50.000 PRIMER_RIGHT_91_GC_PERCENT=54.545 PRIMER_LEFT_91_SELF_ANY=3.00 PRIMER_RIGHT_91_SELF_ANY=3.00 PRIMER_LEFT_91_SELF_END=1.00 PRIMER_RIGHT_91_SELF_END=3.00 PRIMER_LEFT_91_END_STABILITY=8.5000 PRIMER_RIGHT_91_END_STABILITY=6.9000 PRIMER_PAIR_91_COMPL_ANY=4.00 PRIMER_PAIR_91_COMPL_END=3.00 PRIMER_PAIR_91_PRODUCT_SIZE=304 PRIMER_PAIR_92_PENALTY=4.884799 PRIMER_LEFT_92_PENALTY=1.590836 PRIMER_RIGHT_92_PENALTY=3.293963 PRIMER_LEFT_92_SEQUENCE=CAGATCCATCAGGGACCAA PRIMER_RIGHT_92_SEQUENCE=CAAACTCACCAACTAAGCAGC PRIMER_LEFT_92=360,19 PRIMER_RIGHT_92=566,21 PRIMER_LEFT_92_TM=59.409 PRIMER_RIGHT_92_TM=57.706 PRIMER_LEFT_92_GC_PERCENT=52.632 PRIMER_RIGHT_92_GC_PERCENT=47.619 PRIMER_LEFT_92_SELF_ANY=5.00 PRIMER_RIGHT_92_SELF_ANY=3.00 PRIMER_LEFT_92_SELF_END=1.00 PRIMER_RIGHT_92_SELF_END=3.00 PRIMER_LEFT_92_END_STABILITY=8.2000 PRIMER_RIGHT_92_END_STABILITY=9.7000 PRIMER_PAIR_92_COMPL_ANY=2.00 PRIMER_PAIR_92_COMPL_END=0.00 PRIMER_PAIR_92_PRODUCT_SIZE=207 PRIMER_PAIR_93_PENALTY=4.925851 PRIMER_LEFT_93_PENALTY=2.664693 PRIMER_RIGHT_93_PENALTY=2.261158 PRIMER_LEFT_93_SEQUENCE=CTCCGTCAGTTTCCTCGG PRIMER_RIGHT_93_SEQUENCE=CAGTGGGTACCTCCAGCG PRIMER_LEFT_93=46,18 PRIMER_RIGHT_93=266,18 PRIMER_LEFT_93_TM=59.335 PRIMER_RIGHT_93_TM=60.261 PRIMER_LEFT_93_GC_PERCENT=61.111 PRIMER_RIGHT_93_GC_PERCENT=66.667 PRIMER_LEFT_93_SELF_ANY=3.00 PRIMER_RIGHT_93_SELF_ANY=6.00 PRIMER_LEFT_93_SELF_END=2.00 PRIMER_RIGHT_93_SELF_END=2.00 PRIMER_LEFT_93_END_STABILITY=9.9000 PRIMER_RIGHT_93_END_STABILITY=10.2000 PRIMER_PAIR_93_COMPL_ANY=3.00 PRIMER_PAIR_93_COMPL_END=1.00 PRIMER_PAIR_93_PRODUCT_SIZE=221 PRIMER_PAIR_94_PENALTY=4.933547 PRIMER_LEFT_94_PENALTY=1.604227 PRIMER_RIGHT_94_PENALTY=3.329319 PRIMER_LEFT_94_SEQUENCE=GGGATTCAGATCCATCAGGG PRIMER_RIGHT_94_SEQUENCE=TCTTCTCACTGCATGTCTCTTTG PRIMER_LEFT_94=354,20 PRIMER_RIGHT_94=683,23 PRIMER_LEFT_94_TM=61.604 PRIMER_RIGHT_94_TM=59.671 PRIMER_LEFT_94_GC_PERCENT=55.000 PRIMER_RIGHT_94_GC_PERCENT=43.478 PRIMER_LEFT_94_SELF_ANY=5.00 PRIMER_RIGHT_94_SELF_ANY=4.00 PRIMER_LEFT_94_SELF_END=3.00 PRIMER_RIGHT_94_SELF_END=0.00 PRIMER_LEFT_94_END_STABILITY=9.7000 PRIMER_RIGHT_94_END_STABILITY=7.3000 PRIMER_PAIR_94_COMPL_ANY=5.00 PRIMER_PAIR_94_COMPL_END=2.00 PRIMER_PAIR_94_PRODUCT_SIZE=330 PRIMER_PAIR_95_PENALTY=4.947233 PRIMER_LEFT_95_PENALTY=1.604487 PRIMER_RIGHT_95_PENALTY=3.342747 PRIMER_LEFT_95_SEQUENCE=AGGGACCAAAACCTGCATT PRIMER_RIGHT_95_SEQUENCE=GTCTTCACTCCCATCTGCATAGT PRIMER_LEFT_95=370,19 PRIMER_RIGHT_95=868,23 PRIMER_LEFT_95_TM=59.396 PRIMER_RIGHT_95_TM=59.657 PRIMER_LEFT_95_GC_PERCENT=47.368 PRIMER_RIGHT_95_GC_PERCENT=47.826 PRIMER_LEFT_95_SELF_ANY=4.00 PRIMER_RIGHT_95_SELF_ANY=4.00 PRIMER_LEFT_95_SELF_END=1.00 PRIMER_RIGHT_95_SELF_END=2.00 PRIMER_LEFT_95_END_STABILITY=8.4000 PRIMER_RIGHT_95_END_STABILITY=5.3000 PRIMER_PAIR_95_COMPL_ANY=4.00 PRIMER_PAIR_95_COMPL_END=1.00 PRIMER_PAIR_95_PRODUCT_SIZE=499 PRIMER_PAIR_96_PENALTY=5.016847 PRIMER_LEFT_96_PENALTY=1.604487 PRIMER_RIGHT_96_PENALTY=3.412360 PRIMER_LEFT_96_SEQUENCE=GAATGTCCAGAATGGGAAGTG PRIMER_RIGHT_96_SEQUENCE=GGCTGCTGTTGTAGGAAGTTTA PRIMER_LEFT_96=334,21 PRIMER_RIGHT_96=1306,22 PRIMER_LEFT_96_TM=59.396 PRIMER_RIGHT_96_TM=58.588 PRIMER_LEFT_96_GC_PERCENT=47.619 PRIMER_RIGHT_96_GC_PERCENT=45.455 PRIMER_LEFT_96_SELF_ANY=3.00 PRIMER_RIGHT_96_SELF_ANY=3.00 PRIMER_LEFT_96_SELF_END=0.00 PRIMER_RIGHT_96_SELF_END=2.00 PRIMER_LEFT_96_END_STABILITY=6.7000 PRIMER_RIGHT_96_END_STABILITY=6.0000 PRIMER_PAIR_96_COMPL_ANY=3.00 PRIMER_PAIR_96_COMPL_END=0.00 PRIMER_PAIR_96_PRODUCT_SIZE=973 PRIMER_PAIR_97_PENALTY=5.018264 PRIMER_LEFT_97_PENALTY=1.604487 PRIMER_RIGHT_97_PENALTY=3.413777 PRIMER_LEFT_97_SEQUENCE=TGAATGTCCAGAATGGGAAGT PRIMER_RIGHT_97_SEQUENCE=ACAAACTCACCAACTAAGCAGC PRIMER_LEFT_97=333,21 PRIMER_RIGHT_97=567,22 PRIMER_LEFT_97_TM=59.396 PRIMER_RIGHT_97_TM=58.586 PRIMER_LEFT_97_GC_PERCENT=42.857 PRIMER_RIGHT_97_GC_PERCENT=45.455 PRIMER_LEFT_97_SELF_ANY=3.00 PRIMER_RIGHT_97_SELF_ANY=3.00 PRIMER_LEFT_97_SELF_END=1.00 PRIMER_RIGHT_97_SELF_END=3.00 PRIMER_LEFT_97_END_STABILITY=6.4000 PRIMER_RIGHT_97_END_STABILITY=9.7000 PRIMER_PAIR_97_COMPL_ANY=4.00 PRIMER_PAIR_97_COMPL_END=0.00 PRIMER_PAIR_97_PRODUCT_SIZE=235 PRIMER_PAIR_98_PENALTY=5.114433 PRIMER_LEFT_98_PENALTY=1.628165 PRIMER_RIGHT_98_PENALTY=3.486268 PRIMER_LEFT_98_SEQUENCE=TCCAGAATGGGAAGTGGGAT PRIMER_RIGHT_98_SEQUENCE=TCACCAACTAAGCAGCGG PRIMER_LEFT_98=339,20 PRIMER_RIGHT_98=561,18 PRIMER_LEFT_98_TM=61.628 PRIMER_RIGHT_98_TM=58.514 PRIMER_LEFT_98_GC_PERCENT=50.000 PRIMER_RIGHT_98_GC_PERCENT=55.556 PRIMER_LEFT_98_SELF_ANY=3.00 PRIMER_RIGHT_98_SELF_ANY=3.00 PRIMER_LEFT_98_SELF_END=2.00 PRIMER_RIGHT_98_SELF_END=2.00 PRIMER_LEFT_98_END_STABILITY=9.3000 PRIMER_RIGHT_98_END_STABILITY=11.4000 PRIMER_PAIR_98_COMPL_ANY=4.00 PRIMER_PAIR_98_COMPL_END=0.00 PRIMER_PAIR_98_PRODUCT_SIZE=223 PRIMER_PAIR_99_PENALTY=5.146917 PRIMER_LEFT_99_PENALTY=4.984244 PRIMER_RIGHT_99_PENALTY=0.162673 PRIMER_LEFT_99_SEQUENCE=GCTGGAGGTACCCACTGA PRIMER_RIGHT_99_SEQUENCE=CTTGTCAGGAACGAGAAGGG PRIMER_LEFT_99=250,18 PRIMER_RIGHT_99=595,20 PRIMER_LEFT_99_TM=57.016 PRIMER_RIGHT_99_TM=59.837 PRIMER_LEFT_99_GC_PERCENT=61.111 PRIMER_RIGHT_99_GC_PERCENT=55.000 PRIMER_LEFT_99_SELF_ANY=6.00 PRIMER_RIGHT_99_SELF_ANY=4.00 PRIMER_LEFT_99_SELF_END=2.00 PRIMER_RIGHT_99_SELF_END=0.00 PRIMER_LEFT_99_END_STABILITY=6.4000 PRIMER_RIGHT_99_END_STABILITY=9.7000 PRIMER_PAIR_99_COMPL_ANY=4.00 PRIMER_PAIR_99_COMPL_END=0.00 PRIMER_PAIR_99_PRODUCT_SIZE=346 PRIMER_PAIR_100_PENALTY=5.153178 PRIMER_LEFT_100_PENALTY=4.847028 PRIMER_RIGHT_100_PENALTY=0.306150 PRIMER_LEFT_100_SEQUENCE=TTGCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_100_SEQUENCE=TCAAAGTACCAGCGGGAGAT PRIMER_LEFT_100=415,23 PRIMER_RIGHT_100=1128,20 PRIMER_LEFT_100_TM=61.847 PRIMER_RIGHT_100_TM=59.694 PRIMER_LEFT_100_GC_PERCENT=47.826 PRIMER_RIGHT_100_GC_PERCENT=50.000 PRIMER_LEFT_100_SELF_ANY=5.00 PRIMER_RIGHT_100_SELF_ANY=4.00 PRIMER_LEFT_100_SELF_END=0.00 PRIMER_RIGHT_100_SELF_END=2.00 PRIMER_LEFT_100_END_STABILITY=6.7000 PRIMER_RIGHT_100_END_STABILITY=6.3000 PRIMER_PAIR_100_COMPL_ANY=5.00 PRIMER_PAIR_100_COMPL_END=3.00 PRIMER_PAIR_100_PRODUCT_SIZE=714 PRIMER_PAIR_101_PENALTY=5.186407 PRIMER_LEFT_101_PENALTY=4.860451 PRIMER_RIGHT_101_PENALTY=0.325957 PRIMER_LEFT_101_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_101_SEQUENCE=CATTCTCATCCCCAGGTGTC PRIMER_LEFT_101=412,24 PRIMER_RIGHT_101=1358,20 PRIMER_LEFT_101_TM=60.860 PRIMER_RIGHT_101_TM=60.326 PRIMER_LEFT_101_GC_PERCENT=45.833 PRIMER_RIGHT_101_GC_PERCENT=55.000 PRIMER_LEFT_101_SELF_ANY=5.00 PRIMER_RIGHT_101_SELF_ANY=3.00 PRIMER_LEFT_101_SELF_END=1.00 PRIMER_RIGHT_101_SELF_END=1.00 PRIMER_LEFT_101_END_STABILITY=8.2000 PRIMER_RIGHT_101_END_STABILITY=6.1000 PRIMER_PAIR_101_COMPL_ANY=4.00 PRIMER_PAIR_101_COMPL_END=2.00 PRIMER_PAIR_101_PRODUCT_SIZE=947 PRIMER_PAIR_102_PENALTY=5.228019 PRIMER_LEFT_102_PENALTY=2.567306 PRIMER_RIGHT_102_PENALTY=2.660713 PRIMER_LEFT_102_SEQUENCE=GTTTGGCACTGCTCCTGC PRIMER_RIGHT_102_SEQUENCE=GTTCAGGGTAGACTTCTTGGCA PRIMER_LEFT_102=210,18 PRIMER_RIGHT_102=437,22 PRIMER_LEFT_102_TM=60.567 PRIMER_RIGHT_102_TM=60.661 PRIMER_LEFT_102_GC_PERCENT=61.111 PRIMER_RIGHT_102_GC_PERCENT=50.000 PRIMER_LEFT_102_SELF_ANY=5.00 PRIMER_RIGHT_102_SELF_ANY=4.00 PRIMER_LEFT_102_SELF_END=2.00 PRIMER_RIGHT_102_SELF_END=3.00 PRIMER_LEFT_102_END_STABILITY=9.7000 PRIMER_RIGHT_102_END_STABILITY=10.0000 PRIMER_PAIR_102_COMPL_ANY=4.00 PRIMER_PAIR_102_COMPL_END=3.00 PRIMER_PAIR_102_PRODUCT_SIZE=228 PRIMER_PAIR_103_PENALTY=5.234457 PRIMER_LEFT_103_PENALTY=1.608005 PRIMER_RIGHT_103_PENALTY=3.626453 PRIMER_LEFT_103_SEQUENCE=CCTGCAGTATTGCCAAGAAGT PRIMER_RIGHT_103_SEQUENCE=CTTTGTCTTCACTCCCATCTG PRIMER_LEFT_103=406,21 PRIMER_RIGHT_103=872,21 PRIMER_LEFT_103_TM=59.392 PRIMER_RIGHT_103_TM=57.374 PRIMER_LEFT_103_GC_PERCENT=47.619 PRIMER_RIGHT_103_GC_PERCENT=47.619 PRIMER_LEFT_103_SELF_ANY=6.00 PRIMER_RIGHT_103_SELF_ANY=2.00 PRIMER_LEFT_103_SELF_END=1.00 PRIMER_RIGHT_103_SELF_END=2.00 PRIMER_LEFT_103_END_STABILITY=6.4000 PRIMER_RIGHT_103_END_STABILITY=6.6000 PRIMER_PAIR_103_COMPL_ANY=4.00 PRIMER_PAIR_103_COMPL_END=2.00 PRIMER_PAIR_103_PRODUCT_SIZE=467 PRIMER_PAIR_104_PENALTY=5.275172 PRIMER_LEFT_104_PENALTY=1.630431 PRIMER_RIGHT_104_PENALTY=3.644741 PRIMER_LEFT_104_SEQUENCE=TGGCAGACTGAACATGCAC PRIMER_RIGHT_104_SEQUENCE=ACTTTGGGACATGGCGCT PRIMER_LEFT_104=313,19 PRIMER_RIGHT_104=1243,18 PRIMER_LEFT_104_TM=59.370 PRIMER_RIGHT_104_TM=61.645 PRIMER_LEFT_104_GC_PERCENT=52.632 PRIMER_RIGHT_104_GC_PERCENT=55.556 PRIMER_LEFT_104_SELF_ANY=5.00 PRIMER_RIGHT_104_SELF_ANY=4.00 PRIMER_LEFT_104_SELF_END=2.00 PRIMER_RIGHT_104_SELF_END=2.00 PRIMER_LEFT_104_END_STABILITY=8.2000 PRIMER_RIGHT_104_END_STABILITY=11.4000 PRIMER_PAIR_104_COMPL_ANY=4.00 PRIMER_PAIR_104_COMPL_END=1.00 PRIMER_PAIR_104_PRODUCT_SIZE=931 PRIMER_PAIR_105_PENALTY=5.295677 PRIMER_LEFT_105_PENALTY=2.882010 PRIMER_RIGHT_105_PENALTY=2.413667 PRIMER_LEFT_105_SEQUENCE=CTGACTCGCCTGGCTCTG PRIMER_RIGHT_105_SEQUENCE=ATCAGTGGGTACCTCCAGCG PRIMER_LEFT_105=7,18 PRIMER_RIGHT_105=268,20 PRIMER_LEFT_105_TM=60.882 PRIMER_RIGHT_105_TM=62.414 PRIMER_LEFT_105_GC_PERCENT=66.667 PRIMER_RIGHT_105_GC_PERCENT=60.000 PRIMER_LEFT_105_SELF_ANY=5.00 PRIMER_RIGHT_105_SELF_ANY=6.00 PRIMER_LEFT_105_SELF_END=1.00 PRIMER_RIGHT_105_SELF_END=2.00 PRIMER_LEFT_105_END_STABILITY=6.7000 PRIMER_RIGHT_105_END_STABILITY=10.2000 PRIMER_PAIR_105_COMPL_ANY=4.00 PRIMER_PAIR_105_COMPL_END=3.00 PRIMER_PAIR_105_PRODUCT_SIZE=262 PRIMER_PAIR_106_PENALTY=5.322012 PRIMER_LEFT_106_PENALTY=1.630431 PRIMER_RIGHT_106_PENALTY=3.691582 PRIMER_LEFT_106_SEQUENCE=TGTGGCAGACTGAACATGC PRIMER_RIGHT_106_SEQUENCE=ACTTTGTCTTCACTCCCATCTG PRIMER_LEFT_106=311,19 PRIMER_RIGHT_106=873,22 PRIMER_LEFT_106_TM=59.370 PRIMER_RIGHT_106_TM=58.308 PRIMER_LEFT_106_GC_PERCENT=52.632 PRIMER_RIGHT_106_GC_PERCENT=45.455 PRIMER_LEFT_106_SELF_ANY=5.00 PRIMER_RIGHT_106_SELF_ANY=2.00 PRIMER_LEFT_106_SELF_END=2.00 PRIMER_RIGHT_106_SELF_END=2.00 PRIMER_LEFT_106_END_STABILITY=8.4000 PRIMER_RIGHT_106_END_STABILITY=6.6000 PRIMER_PAIR_106_COMPL_ANY=5.00 PRIMER_PAIR_106_COMPL_END=1.00 PRIMER_PAIR_106_PRODUCT_SIZE=563 PRIMER_PAIR_107_PENALTY=5.359258 PRIMER_LEFT_107_PENALTY=1.648882 PRIMER_RIGHT_107_PENALTY=3.710377 PRIMER_LEFT_107_SEQUENCE=AAGTGGGATTCAGATCCATCA PRIMER_RIGHT_107_SEQUENCE=ACTGCATGTCTCTTTGGCGAC PRIMER_LEFT_107=350,21 PRIMER_RIGHT_107=676,21 PRIMER_LEFT_107_TM=59.351 PRIMER_RIGHT_107_TM=62.710 PRIMER_LEFT_107_GC_PERCENT=42.857 PRIMER_RIGHT_107_GC_PERCENT=52.381 PRIMER_LEFT_107_SELF_ANY=5.00 PRIMER_RIGHT_107_SELF_ANY=4.00 PRIMER_LEFT_107_SELF_END=2.00 PRIMER_RIGHT_107_SELF_END=2.00 PRIMER_LEFT_107_END_STABILITY=6.9000 PRIMER_RIGHT_107_END_STABILITY=9.6000 PRIMER_PAIR_107_COMPL_ANY=5.00 PRIMER_PAIR_107_COMPL_END=0.00 PRIMER_PAIR_107_PRODUCT_SIZE=327 PRIMER_PAIR_108_PENALTY=5.379232 PRIMER_LEFT_108_PENALTY=2.710504 PRIMER_RIGHT_108_PENALTY=2.668729 PRIMER_LEFT_108_SEQUENCE=TTTGGCACTGCTCCTGCT PRIMER_RIGHT_108_SEQUENCE=CAGGGTAGACTTCTTGGCAAT PRIMER_LEFT_108=211,18 PRIMER_RIGHT_108=434,21 PRIMER_LEFT_108_TM=60.711 PRIMER_RIGHT_108_TM=58.331 PRIMER_LEFT_108_GC_PERCENT=55.556 PRIMER_RIGHT_108_GC_PERCENT=47.619 PRIMER_LEFT_108_SELF_ANY=5.00 PRIMER_RIGHT_108_SELF_ANY=5.00 PRIMER_LEFT_108_SELF_END=0.00 PRIMER_RIGHT_108_SELF_END=3.00 PRIMER_LEFT_108_END_STABILITY=8.2000 PRIMER_RIGHT_108_END_STABILITY=8.4000 PRIMER_PAIR_108_COMPL_ANY=4.00 PRIMER_PAIR_108_COMPL_END=0.00 PRIMER_PAIR_108_PRODUCT_SIZE=224 PRIMER_PAIR_109_PENALTY=5.478782 PRIMER_LEFT_109_PENALTY=1.651669 PRIMER_RIGHT_109_PENALTY=3.827113 PRIMER_LEFT_109_SEQUENCE=TCCATCAGGGACCAAAACCT PRIMER_RIGHT_109_SEQUENCE=TGTCTTCACTCCCATCTGC PRIMER_LEFT_109=364,20 PRIMER_RIGHT_109=869,19 PRIMER_LEFT_109_TM=61.652 PRIMER_RIGHT_109_TM=57.173 PRIMER_LEFT_109_GC_PERCENT=50.000 PRIMER_RIGHT_109_GC_PERCENT=52.632 PRIMER_LEFT_109_SELF_ANY=5.00 PRIMER_RIGHT_109_SELF_ANY=2.00 PRIMER_LEFT_109_SELF_END=0.00 PRIMER_RIGHT_109_SELF_END=2.00 PRIMER_LEFT_109_END_STABILITY=7.9000 PRIMER_RIGHT_109_END_STABILITY=8.2000 PRIMER_PAIR_109_COMPL_ANY=4.00 PRIMER_PAIR_109_COMPL_END=0.00 PRIMER_PAIR_109_PRODUCT_SIZE=506 PRIMER_PAIR_110_PENALTY=5.566446 PRIMER_LEFT_110_PENALTY=1.659162 PRIMER_RIGHT_110_PENALTY=3.907284 PRIMER_LEFT_110_SEQUENCE=CATGTTCTGTGGCAGACTGA PRIMER_RIGHT_110_SEQUENCE=TCAGAGCACACCTCTCGAACC PRIMER_LEFT_110=304,20 PRIMER_RIGHT_110=1077,21 PRIMER_LEFT_110_TM=58.341 PRIMER_RIGHT_110_TM=62.907 PRIMER_LEFT_110_GC_PERCENT=50.000 PRIMER_RIGHT_110_GC_PERCENT=57.143 PRIMER_LEFT_110_SELF_ANY=5.00 PRIMER_RIGHT_110_SELF_ANY=4.00 PRIMER_LEFT_110_SELF_END=3.00 PRIMER_RIGHT_110_SELF_END=0.00 PRIMER_LEFT_110_END_STABILITY=6.4000 PRIMER_RIGHT_110_END_STABILITY=7.9000 PRIMER_PAIR_110_COMPL_ANY=5.00 PRIMER_PAIR_110_COMPL_END=0.00 PRIMER_PAIR_110_PRODUCT_SIZE=774 PRIMER_PAIR_111_PENALTY=5.584852 PRIMER_LEFT_111_PENALTY=1.675600 PRIMER_RIGHT_111_PENALTY=3.909253 PRIMER_LEFT_111_SEQUENCE=TGGAGGTACCCACTGATGG PRIMER_RIGHT_111_SEQUENCE=AACTTTGGGACATGGCGCT PRIMER_LEFT_111=252,19 PRIMER_RIGHT_111=1244,19 PRIMER_LEFT_111_TM=59.324 PRIMER_RIGHT_111_TM=62.909 PRIMER_LEFT_111_GC_PERCENT=57.895 PRIMER_RIGHT_111_GC_PERCENT=52.632 PRIMER_LEFT_111_SELF_ANY=6.00 PRIMER_RIGHT_111_SELF_ANY=4.00 PRIMER_LEFT_111_SELF_END=3.00 PRIMER_RIGHT_111_SELF_END=2.00 PRIMER_LEFT_111_END_STABILITY=8.1000 PRIMER_RIGHT_111_END_STABILITY=11.4000 PRIMER_PAIR_111_COMPL_ANY=4.00 PRIMER_PAIR_111_COMPL_END=0.00 PRIMER_PAIR_111_PRODUCT_SIZE=993 PRIMER_PAIR_112_PENALTY=5.597872 PRIMER_LEFT_112_PENALTY=5.291723 PRIMER_RIGHT_112_PENALTY=0.306150 PRIMER_LEFT_112_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_112_SEQUENCE=ATCAAAGTACCAGCGGGAGA PRIMER_LEFT_112=413,24 PRIMER_RIGHT_112=1129,20 PRIMER_LEFT_112_TM=61.292 PRIMER_RIGHT_112_TM=59.694 PRIMER_LEFT_112_GC_PERCENT=41.667 PRIMER_RIGHT_112_GC_PERCENT=50.000 PRIMER_LEFT_112_SELF_ANY=5.00 PRIMER_RIGHT_112_SELF_ANY=4.00 PRIMER_LEFT_112_SELF_END=0.00 PRIMER_RIGHT_112_SELF_END=0.00 PRIMER_LEFT_112_END_STABILITY=7.0000 PRIMER_RIGHT_112_END_STABILITY=7.9000 PRIMER_PAIR_112_COMPL_ANY=5.00 PRIMER_PAIR_112_COMPL_END=3.00 PRIMER_PAIR_112_PRODUCT_SIZE=717 PRIMER_PAIR_113_PENALTY=5.649800 PRIMER_LEFT_113_PENALTY=1.676675 PRIMER_RIGHT_113_PENALTY=3.973125 PRIMER_LEFT_113_SEQUENCE=GGATTCAGATCCATCAGGGAC PRIMER_RIGHT_113_SEQUENCE=TTGTCTTCACTCCCATCTGCAT PRIMER_LEFT_113=355,21 PRIMER_RIGHT_113=870,22 PRIMER_LEFT_113_TM=60.677 PRIMER_RIGHT_113_TM=61.973 PRIMER_LEFT_113_GC_PERCENT=52.381 PRIMER_RIGHT_113_GC_PERCENT=45.455 PRIMER_LEFT_113_SELF_ANY=5.00 PRIMER_RIGHT_113_SELF_ANY=4.00 PRIMER_LEFT_113_SELF_END=3.00 PRIMER_RIGHT_113_SELF_END=2.00 PRIMER_LEFT_113_END_STABILITY=9.1000 PRIMER_RIGHT_113_END_STABILITY=8.4000 PRIMER_PAIR_113_COMPL_ANY=6.00 PRIMER_PAIR_113_COMPL_END=3.00 PRIMER_PAIR_113_PRODUCT_SIZE=516 PRIMER_PAIR_114_PENALTY=5.735954 PRIMER_LEFT_114_PENALTY=5.409997 PRIMER_RIGHT_114_PENALTY=0.325957 PRIMER_LEFT_114_SEQUENCE=AGTATTGCCAAGAAGTCTACCCT PRIMER_RIGHT_114_SEQUENCE=TCATTCTCATCCCCAGGTGT PRIMER_LEFT_114=411,23 PRIMER_RIGHT_114=1359,20 PRIMER_LEFT_114_TM=57.590 PRIMER_RIGHT_114_TM=60.326 PRIMER_LEFT_114_GC_PERCENT=43.478 PRIMER_RIGHT_114_GC_PERCENT=50.000 PRIMER_LEFT_114_SELF_ANY=5.00 PRIMER_RIGHT_114_SELF_ANY=3.00 PRIMER_LEFT_114_SELF_END=2.00 PRIMER_RIGHT_114_SELF_END=1.00 PRIMER_LEFT_114_END_STABILITY=9.1000 PRIMER_RIGHT_114_END_STABILITY=7.6000 PRIMER_PAIR_114_COMPL_ANY=4.00 PRIMER_PAIR_114_COMPL_END=3.00 PRIMER_PAIR_114_PRODUCT_SIZE=949 PRIMER_PAIR_115_PENALTY=5.795712 PRIMER_LEFT_115_PENALTY=1.683804 PRIMER_RIGHT_115_PENALTY=4.111908 PRIMER_LEFT_115_SEQUENCE=ACCCCAGATTGCCATGTTCT PRIMER_RIGHT_115_SEQUENCE=TTACAAACTCACCAACTAAGCAGC PRIMER_LEFT_115=292,20 PRIMER_RIGHT_115=569,24 PRIMER_LEFT_115_TM=61.684 PRIMER_RIGHT_115_TM=59.888 PRIMER_LEFT_115_GC_PERCENT=50.000 PRIMER_RIGHT_115_GC_PERCENT=41.667 PRIMER_LEFT_115_SELF_ANY=4.00 PRIMER_RIGHT_115_SELF_ANY=3.00 PRIMER_LEFT_115_SELF_END=0.00 PRIMER_RIGHT_115_SELF_END=3.00 PRIMER_LEFT_115_END_STABILITY=6.4000 PRIMER_RIGHT_115_END_STABILITY=9.7000 PRIMER_PAIR_115_COMPL_ANY=4.00 PRIMER_PAIR_115_COMPL_END=1.00 PRIMER_PAIR_115_PRODUCT_SIZE=278 PRIMER_PAIR_116_PENALTY=5.819620 PRIMER_LEFT_116_PENALTY=1.707711 PRIMER_RIGHT_116_PENALTY=4.111908 PRIMER_LEFT_116_SEQUENCE=CATGAATGTCCAGAATGGGAA PRIMER_RIGHT_116_SEQUENCE=GCTGCTGTTGTAGGAAGTTTAACA PRIMER_LEFT_116=331,21 PRIMER_RIGHT_116=1305,24 PRIMER_LEFT_116_TM=60.708 PRIMER_RIGHT_116_TM=59.888 PRIMER_LEFT_116_GC_PERCENT=42.857 PRIMER_RIGHT_116_GC_PERCENT=41.667 PRIMER_LEFT_116_SELF_ANY=4.00 PRIMER_RIGHT_116_SELF_ANY=5.00 PRIMER_LEFT_116_SELF_END=1.00 PRIMER_RIGHT_116_SELF_END=3.00 PRIMER_LEFT_116_END_STABILITY=9.7000 PRIMER_RIGHT_116_END_STABILITY=6.0000 PRIMER_PAIR_116_COMPL_ANY=3.00 PRIMER_PAIR_116_COMPL_END=2.00 PRIMER_PAIR_116_PRODUCT_SIZE=975 PRIMER_PAIR_117_PENALTY=5.865112 PRIMER_LEFT_117_PENALTY=1.709051 PRIMER_RIGHT_117_PENALTY=4.156061 PRIMER_LEFT_117_SEQUENCE=TCCATCAGGGACCAAAACC PRIMER_RIGHT_117_SEQUENCE=TGGCTGCTGTTGTAGGAAGTTTA PRIMER_LEFT_117=364,19 PRIMER_RIGHT_117=1307,23 PRIMER_LEFT_117_TM=60.709 PRIMER_RIGHT_117_TM=61.156 PRIMER_LEFT_117_GC_PERCENT=52.632 PRIMER_RIGHT_117_GC_PERCENT=43.478 PRIMER_LEFT_117_SELF_ANY=5.00 PRIMER_RIGHT_117_SELF_ANY=3.00 PRIMER_LEFT_117_SELF_END=0.00 PRIMER_RIGHT_117_SELF_END=2.00 PRIMER_LEFT_117_END_STABILITY=8.2000 PRIMER_RIGHT_117_END_STABILITY=6.0000 PRIMER_PAIR_117_COMPL_ANY=4.00 PRIMER_PAIR_117_COMPL_END=2.00 PRIMER_PAIR_117_PRODUCT_SIZE=944 PRIMER_PAIR_118_PENALTY=5.939864 PRIMER_LEFT_118_PENALTY=1.741437 PRIMER_RIGHT_118_PENALTY=4.198427 PRIMER_LEFT_118_SEQUENCE=ACCCCAGATTGCCATGTTC PRIMER_RIGHT_118_SEQUENCE=CAAACTCACCAACTAAGCAGCG PRIMER_LEFT_118=292,19 PRIMER_RIGHT_118=566,22 PRIMER_LEFT_118_TM=60.741 PRIMER_RIGHT_118_TM=62.198 PRIMER_LEFT_118_GC_PERCENT=52.632 PRIMER_RIGHT_118_GC_PERCENT=50.000 PRIMER_LEFT_118_SELF_ANY=4.00 PRIMER_RIGHT_118_SELF_ANY=3.00 PRIMER_LEFT_118_SELF_END=0.00 PRIMER_RIGHT_118_SELF_END=2.00 PRIMER_LEFT_118_END_STABILITY=6.7000 PRIMER_RIGHT_118_END_STABILITY=10.2000 PRIMER_PAIR_118_COMPL_ANY=4.00 PRIMER_PAIR_118_COMPL_END=1.00 PRIMER_PAIR_118_PRODUCT_SIZE=275 PRIMER_PAIR_119_PENALTY=5.966723 PRIMER_LEFT_119_PENALTY=1.757583 PRIMER_RIGHT_119_PENALTY=4.209140 PRIMER_LEFT_119_SEQUENCE=ATGGGAAGTGGGATTCAGAT PRIMER_RIGHT_119_SEQUENCE=AGCACACCTCTCGAACCA PRIMER_LEFT_119=345,20 PRIMER_RIGHT_119=1073,18 PRIMER_LEFT_119_TM=58.242 PRIMER_RIGHT_119_TM=57.791 PRIMER_LEFT_119_GC_PERCENT=45.000 PRIMER_RIGHT_119_GC_PERCENT=55.556 PRIMER_LEFT_119_SELF_ANY=3.00 PRIMER_RIGHT_119_SELF_ANY=4.00 PRIMER_LEFT_119_SELF_END=2.00 PRIMER_RIGHT_119_SELF_END=0.00 PRIMER_LEFT_119_END_STABILITY=6.6000 PRIMER_RIGHT_119_END_STABILITY=8.2000 PRIMER_PAIR_119_COMPL_ANY=4.00 PRIMER_PAIR_119_COMPL_END=2.00 PRIMER_PAIR_119_PRODUCT_SIZE=729 PRIMER_PAIR_120_PENALTY=6.039199 PRIMER_LEFT_120_PENALTY=1.798843 PRIMER_RIGHT_120_PENALTY=4.240356 PRIMER_LEFT_120_SEQUENCE=ACATGAATGTCCAGAATGGG PRIMER_RIGHT_120_SEQUENCE=CTTCTCACTGCATGTCTCTTTG PRIMER_LEFT_120=330,20 PRIMER_RIGHT_120=682,22 PRIMER_LEFT_120_TM=58.201 PRIMER_RIGHT_120_TM=57.760 PRIMER_LEFT_120_GC_PERCENT=45.000 PRIMER_RIGHT_120_GC_PERCENT=45.455 PRIMER_LEFT_120_SELF_ANY=6.00 PRIMER_RIGHT_120_SELF_ANY=4.00 PRIMER_LEFT_120_SELF_END=1.00 PRIMER_RIGHT_120_SELF_END=0.00 PRIMER_LEFT_120_END_STABILITY=9.6000 PRIMER_RIGHT_120_END_STABILITY=7.3000 PRIMER_PAIR_120_COMPL_ANY=6.00 PRIMER_PAIR_120_COMPL_END=1.00 PRIMER_PAIR_120_PRODUCT_SIZE=353 PRIMER_PAIR_121_PENALTY=6.052391 PRIMER_LEFT_121_PENALTY=2.974589 PRIMER_RIGHT_121_PENALTY=3.077802 PRIMER_LEFT_121_SEQUENCE=GGATCAGCTGACTCGCCT PRIMER_RIGHT_121_SEQUENCE=GTTCAGGGTAGACTTCTTGGC PRIMER_LEFT_121=0,18 PRIMER_RIGHT_121=437,21 PRIMER_LEFT_121_TM=59.025 PRIMER_RIGHT_121_TM=57.922 PRIMER_LEFT_121_GC_PERCENT=61.111 PRIMER_RIGHT_121_GC_PERCENT=52.381 PRIMER_LEFT_121_SELF_ANY=8.00 PRIMER_RIGHT_121_SELF_ANY=4.00 PRIMER_LEFT_121_SELF_END=3.00 PRIMER_RIGHT_121_SELF_END=2.00 PRIMER_LEFT_121_END_STABILITY=11.4000 PRIMER_RIGHT_121_END_STABILITY=10.0000 PRIMER_PAIR_121_COMPL_ANY=4.00 PRIMER_PAIR_121_COMPL_END=2.00 PRIMER_PAIR_121_PRODUCT_SIZE=438 PRIMER_PAIR_122_PENALTY=6.106046 PRIMER_LEFT_122_PENALTY=3.028072 PRIMER_RIGHT_122_PENALTY=3.077974 PRIMER_LEFT_122_SEQUENCE=ATCCCACTCGCACAGCAG PRIMER_RIGHT_122_SEQUENCE=AGTTCAGGGTAGACTTCTTGGC PRIMER_LEFT_122=154,18 PRIMER_RIGHT_122=438,22 PRIMER_LEFT_122_TM=61.028 PRIMER_RIGHT_122_TM=58.922 PRIMER_LEFT_122_GC_PERCENT=61.111 PRIMER_RIGHT_122_GC_PERCENT=50.000 PRIMER_LEFT_122_SELF_ANY=2.00 PRIMER_RIGHT_122_SELF_ANY=4.00 PRIMER_LEFT_122_SELF_END=2.00 PRIMER_RIGHT_122_SELF_END=2.00 PRIMER_LEFT_122_END_STABILITY=8.2000 PRIMER_RIGHT_122_END_STABILITY=10.0000 PRIMER_PAIR_122_COMPL_ANY=3.00 PRIMER_PAIR_122_COMPL_END=3.00 PRIMER_PAIR_122_PRODUCT_SIZE=285 PRIMER_PAIR_123_PENALTY=6.149183 PRIMER_LEFT_123_PENALTY=1.907724 PRIMER_RIGHT_123_PENALTY=4.241458 PRIMER_LEFT_123_SEQUENCE=CAGGGACCAAAACCTGCAT PRIMER_RIGHT_123_SEQUENCE=TGCTGTTGTAGGAAGTTTAACAGG PRIMER_LEFT_123=369,19 PRIMER_RIGHT_123=1303,24 PRIMER_LEFT_123_TM=60.908 PRIMER_RIGHT_123_TM=59.759 PRIMER_LEFT_123_GC_PERCENT=52.632 PRIMER_RIGHT_123_GC_PERCENT=41.667 PRIMER_LEFT_123_SELF_ANY=4.00 PRIMER_RIGHT_123_SELF_ANY=5.00 PRIMER_LEFT_123_SELF_END=2.00 PRIMER_RIGHT_123_SELF_END=1.00 PRIMER_LEFT_123_END_STABILITY=8.4000 PRIMER_RIGHT_123_END_STABILITY=7.9000 PRIMER_PAIR_123_COMPL_ANY=4.00 PRIMER_PAIR_123_COMPL_END=2.00 PRIMER_PAIR_123_PRODUCT_SIZE=935 PRIMER_PAIR_124_PENALTY=6.152874 PRIMER_LEFT_124_PENALTY=1.811785 PRIMER_RIGHT_124_PENALTY=4.341090 PRIMER_LEFT_124_SEQUENCE=GGTACCCACTGATGGTAATGC PRIMER_RIGHT_124_SEQUENCE=CTTCTCACTGCATGTCTCTTTGG PRIMER_LEFT_124=256,21 PRIMER_RIGHT_124=682,23 PRIMER_LEFT_124_TM=59.188 PRIMER_RIGHT_124_TM=61.341 PRIMER_LEFT_124_GC_PERCENT=52.381 PRIMER_RIGHT_124_GC_PERCENT=47.826 PRIMER_LEFT_124_SELF_ANY=6.00 PRIMER_RIGHT_124_SELF_ANY=4.00 PRIMER_LEFT_124_SELF_END=2.00 PRIMER_RIGHT_124_SELF_END=0.00 PRIMER_LEFT_124_END_STABILITY=8.4000 PRIMER_RIGHT_124_END_STABILITY=8.8000 PRIMER_PAIR_124_COMPL_ANY=4.00 PRIMER_PAIR_124_COMPL_END=2.00 PRIMER_PAIR_124_PRODUCT_SIZE=427 PRIMER_PAIR_125_PENALTY=6.157949 PRIMER_LEFT_125_PENALTY=5.821907 PRIMER_RIGHT_125_PENALTY=0.336042 PRIMER_LEFT_125_SEQUENCE=TGCAGTATTGCCAAGAAGTCTACC PRIMER_RIGHT_125_SEQUENCE=GGTTCCTCAGCCTCTTCCTC PRIMER_LEFT_125=408,24 PRIMER_RIGHT_125=981,20 PRIMER_LEFT_125_TM=61.822 PRIMER_RIGHT_125_TM=60.336 PRIMER_LEFT_125_GC_PERCENT=45.833 PRIMER_RIGHT_125_GC_PERCENT=60.000 PRIMER_LEFT_125_SELF_ANY=6.00 PRIMER_RIGHT_125_SELF_ANY=3.00 PRIMER_LEFT_125_SELF_END=1.00 PRIMER_RIGHT_125_SELF_END=0.00 PRIMER_LEFT_125_END_STABILITY=6.9000 PRIMER_RIGHT_125_END_STABILITY=7.9000 PRIMER_PAIR_125_COMPL_ANY=4.00 PRIMER_PAIR_125_COMPL_END=0.00 PRIMER_PAIR_125_PRODUCT_SIZE=574 PRIMER_PAIR_126_PENALTY=6.229736 PRIMER_LEFT_126_PENALTY=1.876405 PRIMER_RIGHT_126_PENALTY=4.353331 PRIMER_LEFT_126_SEQUENCE=CCCCAGATTGCCATGTTCT PRIMER_RIGHT_126_SEQUENCE=TACAAACTCACCAACTAAGCAGC PRIMER_LEFT_126=293,19 PRIMER_RIGHT_126=568,23 PRIMER_LEFT_126_TM=60.876 PRIMER_RIGHT_126_TM=58.647 PRIMER_LEFT_126_GC_PERCENT=52.632 PRIMER_RIGHT_126_GC_PERCENT=43.478 PRIMER_LEFT_126_SELF_ANY=4.00 PRIMER_RIGHT_126_SELF_ANY=3.00 PRIMER_LEFT_126_SELF_END=0.00 PRIMER_RIGHT_126_SELF_END=3.00 PRIMER_LEFT_126_END_STABILITY=6.4000 PRIMER_RIGHT_126_END_STABILITY=9.7000 PRIMER_PAIR_126_COMPL_ANY=4.00 PRIMER_PAIR_126_COMPL_END=1.00 PRIMER_PAIR_126_PRODUCT_SIZE=276 PRIMER_PAIR_127_PENALTY=6.284438 PRIMER_LEFT_127_PENALTY=5.925828 PRIMER_RIGHT_127_PENALTY=0.358609 PRIMER_LEFT_127_SEQUENCE=CCTGCAGTATTGCCAAGAAGTCTA PRIMER_RIGHT_127_SEQUENCE=CAGTGGGCAACACACAAACT PRIMER_LEFT_127=406,24 PRIMER_RIGHT_127=766,20 PRIMER_LEFT_127_TM=61.926 PRIMER_RIGHT_127_TM=59.641 PRIMER_LEFT_127_GC_PERCENT=45.833 PRIMER_RIGHT_127_GC_PERCENT=50.000 PRIMER_LEFT_127_SELF_ANY=6.00 PRIMER_RIGHT_127_SELF_ANY=3.00 PRIMER_LEFT_127_SELF_END=2.00 PRIMER_RIGHT_127_SELF_END=1.00 PRIMER_LEFT_127_END_STABILITY=5.4000 PRIMER_RIGHT_127_END_STABILITY=6.7000 PRIMER_PAIR_127_COMPL_ANY=6.00 PRIMER_PAIR_127_COMPL_END=2.00 PRIMER_PAIR_127_PRODUCT_SIZE=361 PRIMER_PAIR_128_PENALTY=6.362197 PRIMER_LEFT_128_PENALTY=6.000430 PRIMER_RIGHT_128_PENALTY=0.361767 PRIMER_LEFT_128_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_128_SEQUENCE=CGTCATCATCGGCTTCTTCT PRIMER_LEFT_128=414,24 PRIMER_RIGHT_128=935,20 PRIMER_LEFT_128_TM=62.000 PRIMER_RIGHT_128_TM=60.362 PRIMER_LEFT_128_GC_PERCENT=45.833 PRIMER_RIGHT_128_GC_PERCENT=50.000 PRIMER_LEFT_128_SELF_ANY=5.00 PRIMER_RIGHT_128_SELF_ANY=3.00 PRIMER_LEFT_128_SELF_END=0.00 PRIMER_RIGHT_128_SELF_END=0.00 PRIMER_LEFT_128_END_STABILITY=6.7000 PRIMER_RIGHT_128_END_STABILITY=6.7000 PRIMER_PAIR_128_COMPL_ANY=6.00 PRIMER_PAIR_128_COMPL_END=3.00 PRIMER_PAIR_128_PRODUCT_SIZE=522 PRIMER_PAIR_129_PENALTY=6.380664 PRIMER_LEFT_129_PENALTY=3.124765 PRIMER_RIGHT_129_PENALTY=3.255899 PRIMER_LEFT_129_SEQUENCE=GGGCTCCGTCAGTTTCCTC PRIMER_RIGHT_129_SEQUENCE=TCAGTGGGTACCTCCAGCG PRIMER_LEFT_129=43,19 PRIMER_RIGHT_129=267,19 PRIMER_LEFT_129_TM=62.125 PRIMER_RIGHT_129_TM=62.256 PRIMER_LEFT_129_GC_PERCENT=63.158 PRIMER_RIGHT_129_GC_PERCENT=63.158 PRIMER_LEFT_129_SELF_ANY=2.00 PRIMER_RIGHT_129_SELF_ANY=6.00 PRIMER_LEFT_129_SELF_END=0.00 PRIMER_RIGHT_129_SELF_END=2.00 PRIMER_LEFT_129_END_STABILITY=7.9000 PRIMER_RIGHT_129_END_STABILITY=10.2000 PRIMER_PAIR_129_COMPL_ANY=3.00 PRIMER_PAIR_129_COMPL_END=1.00 PRIMER_PAIR_129_PRODUCT_SIZE=225 PRIMER_PAIR_130_PENALTY=6.439739 PRIMER_LEFT_130_PENALTY=2.051254 PRIMER_RIGHT_130_PENALTY=4.388485 PRIMER_LEFT_130_SEQUENCE=AACCCCAGATTGCCATGTTC PRIMER_RIGHT_130_SEQUENCE=GCACACCTCTCGAACCAC PRIMER_LEFT_130=291,20 PRIMER_RIGHT_130=1072,18 PRIMER_LEFT_130_TM=62.051 PRIMER_RIGHT_130_TM=57.612 PRIMER_LEFT_130_GC_PERCENT=50.000 PRIMER_RIGHT_130_GC_PERCENT=61.111 PRIMER_LEFT_130_SELF_ANY=4.00 PRIMER_RIGHT_130_SELF_ANY=4.00 PRIMER_LEFT_130_SELF_END=0.00 PRIMER_RIGHT_130_SELF_END=0.00 PRIMER_LEFT_130_END_STABILITY=6.7000 PRIMER_RIGHT_130_END_STABILITY=7.6000 PRIMER_PAIR_130_COMPL_ANY=4.00 PRIMER_PAIR_130_COMPL_END=2.00 PRIMER_PAIR_130_PRODUCT_SIZE=782 PRIMER_PAIR_131_PENALTY=6.463179 PRIMER_LEFT_131_PENALTY=2.051254 PRIMER_RIGHT_131_PENALTY=4.411925 PRIMER_LEFT_131_SEQUENCE=GAACCCCAGATTGCCATGTT PRIMER_RIGHT_131_SEQUENCE=AACTCACCAACTAAGCAGCGGT PRIMER_LEFT_131=290,20 PRIMER_RIGHT_131=564,22 PRIMER_LEFT_131_TM=62.051 PRIMER_RIGHT_131_TM=62.412 PRIMER_LEFT_131_GC_PERCENT=50.000 PRIMER_RIGHT_131_GC_PERCENT=50.000 PRIMER_LEFT_131_SELF_ANY=4.00 PRIMER_RIGHT_131_SELF_ANY=3.00 PRIMER_LEFT_131_SELF_END=2.00 PRIMER_RIGHT_131_SELF_END=2.00 PRIMER_LEFT_131_END_STABILITY=6.6000 PRIMER_RIGHT_131_END_STABILITY=11.1000 PRIMER_PAIR_131_COMPL_ANY=4.00 PRIMER_PAIR_131_COMPL_END=1.00 PRIMER_PAIR_131_PRODUCT_SIZE=275 PRIMER_PAIR_132_PENALTY=6.473323 PRIMER_LEFT_132_PENALTY=2.056428 PRIMER_RIGHT_132_PENALTY=4.416895 PRIMER_LEFT_132_SEQUENCE=CCAAAACCTGCATTGATACC PRIMER_RIGHT_132_SEQUENCE=GTAGGAAGTTTAACAGGATCTCGG PRIMER_LEFT_132=375,20 PRIMER_RIGHT_132=1296,24 PRIMER_LEFT_132_TM=57.944 PRIMER_RIGHT_132_TM=59.583 PRIMER_LEFT_132_GC_PERCENT=45.000 PRIMER_RIGHT_132_GC_PERCENT=45.833 PRIMER_LEFT_132_SELF_ANY=4.00 PRIMER_RIGHT_132_SELF_ANY=5.00 PRIMER_LEFT_132_SELF_END=2.00 PRIMER_RIGHT_132_SELF_END=2.00 PRIMER_LEFT_132_END_STABILITY=6.8000 PRIMER_RIGHT_132_END_STABILITY=9.9000 PRIMER_PAIR_132_COMPL_ANY=4.00 PRIMER_PAIR_132_COMPL_END=2.00 PRIMER_PAIR_132_PRODUCT_SIZE=922 PRIMER_PAIR_133_PENALTY=6.533439 PRIMER_LEFT_133_PENALTY=2.071163 PRIMER_RIGHT_133_PENALTY=4.462276 PRIMER_LEFT_133_SEQUENCE=ATTGCCATGTTCTGTGGCAG PRIMER_RIGHT_133_SEQUENCE=CTTGTTCAGAGCACACCTCTCG PRIMER_LEFT_133=299,20 PRIMER_RIGHT_133=1082,22 PRIMER_LEFT_133_TM=62.071 PRIMER_RIGHT_133_TM=62.462 PRIMER_LEFT_133_GC_PERCENT=50.000 PRIMER_RIGHT_133_GC_PERCENT=54.545 PRIMER_LEFT_133_SELF_ANY=5.00 PRIMER_RIGHT_133_SELF_ANY=5.00 PRIMER_LEFT_133_SELF_END=3.00 PRIMER_RIGHT_133_SELF_END=2.00 PRIMER_LEFT_133_END_STABILITY=9.7000 PRIMER_RIGHT_133_END_STABILITY=8.4000 PRIMER_PAIR_133_COMPL_ANY=5.00 PRIMER_PAIR_133_COMPL_END=1.00 PRIMER_PAIR_133_PRODUCT_SIZE=784 PRIMER_PAIR_134_PENALTY=6.622260 PRIMER_LEFT_134_PENALTY=2.083070 PRIMER_RIGHT_134_PENALTY=4.539189 PRIMER_LEFT_134_SEQUENCE=CATTGATACCAAGGAAGGCAT PRIMER_RIGHT_134_SEQUENCE=GGTACTCTTCTCACTGCATGTCTC PRIMER_LEFT_134=385,21 PRIMER_RIGHT_134=688,24 PRIMER_LEFT_134_TM=58.917 PRIMER_RIGHT_134_TM=59.461 PRIMER_LEFT_134_GC_PERCENT=42.857 PRIMER_RIGHT_134_GC_PERCENT=50.000 PRIMER_LEFT_134_SELF_ANY=6.00 PRIMER_RIGHT_134_SELF_ANY=4.00 PRIMER_LEFT_134_SELF_END=2.00 PRIMER_RIGHT_134_SELF_END=0.00 PRIMER_LEFT_134_END_STABILITY=9.6000 PRIMER_RIGHT_134_END_STABILITY=6.1000 PRIMER_PAIR_134_COMPL_ANY=4.00 PRIMER_PAIR_134_COMPL_END=3.00 PRIMER_PAIR_134_PRODUCT_SIZE=304 PRIMER_PAIR_135_PENALTY=6.631312 PRIMER_LEFT_135_PENALTY=3.128205 PRIMER_RIGHT_135_PENALTY=3.503107 PRIMER_LEFT_135_SEQUENCE=GATCAGCTGACTCGCCTGG PRIMER_RIGHT_135_SEQUENCE=ATCAGTGGGTACCTCCAGC PRIMER_LEFT_135=1,19 PRIMER_RIGHT_135=268,19 PRIMER_LEFT_135_TM=62.128 PRIMER_RIGHT_135_TM=57.497 PRIMER_LEFT_135_GC_PERCENT=63.158 PRIMER_RIGHT_135_GC_PERCENT=57.895 PRIMER_LEFT_135_SELF_ANY=8.00 PRIMER_RIGHT_135_SELF_ANY=6.00 PRIMER_LEFT_135_SELF_END=3.00 PRIMER_RIGHT_135_SELF_END=2.00 PRIMER_LEFT_135_END_STABILITY=9.7000 PRIMER_RIGHT_135_END_STABILITY=9.7000 PRIMER_PAIR_135_COMPL_ANY=4.00 PRIMER_PAIR_135_COMPL_END=3.00 PRIMER_PAIR_135_PRODUCT_SIZE=268 PRIMER_PAIR_136_PENALTY=6.644573 PRIMER_LEFT_136_PENALTY=2.085443 PRIMER_RIGHT_136_PENALTY=4.559130 PRIMER_LEFT_136_SEQUENCE=CATCAGGGACCAAAACCTG PRIMER_RIGHT_136_SEQUENCE=TGTCTTCACTCCCATCTGCATAG PRIMER_LEFT_136=366,19 PRIMER_RIGHT_136=869,23 PRIMER_LEFT_136_TM=58.915 PRIMER_RIGHT_136_TM=61.559 PRIMER_LEFT_136_GC_PERCENT=52.632 PRIMER_RIGHT_136_GC_PERCENT=47.826 PRIMER_LEFT_136_SELF_ANY=4.00 PRIMER_RIGHT_136_SELF_ANY=4.00 PRIMER_LEFT_136_SELF_END=1.00 PRIMER_RIGHT_136_SELF_END=2.00 PRIMER_LEFT_136_END_STABILITY=7.9000 PRIMER_RIGHT_136_END_STABILITY=5.9000 PRIMER_PAIR_136_COMPL_ANY=4.00 PRIMER_PAIR_136_COMPL_END=1.00 PRIMER_PAIR_136_PRODUCT_SIZE=504 PRIMER_PAIR_137_PENALTY=6.682945 PRIMER_LEFT_137_PENALTY=2.118737 PRIMER_RIGHT_137_PENALTY=4.564208 PRIMER_LEFT_137_SEQUENCE=AATGTCCAGAATGGGAAGTGG PRIMER_RIGHT_137_SEQUENCE=CACACCTCTCGAACCACC PRIMER_LEFT_137=335,21 PRIMER_RIGHT_137=1071,18 PRIMER_LEFT_137_TM=61.119 PRIMER_RIGHT_137_TM=57.436 PRIMER_LEFT_137_GC_PERCENT=47.619 PRIMER_RIGHT_137_GC_PERCENT=61.111 PRIMER_LEFT_137_SELF_ANY=3.00 PRIMER_RIGHT_137_SELF_ANY=4.00 PRIMER_LEFT_137_SELF_END=0.00 PRIMER_RIGHT_137_SELF_END=0.00 PRIMER_LEFT_137_END_STABILITY=7.9000 PRIMER_RIGHT_137_END_STABILITY=9.4000 PRIMER_PAIR_137_COMPL_ANY=4.00 PRIMER_PAIR_137_COMPL_END=3.00 PRIMER_PAIR_137_PRODUCT_SIZE=737 PRIMER_PAIR_138_PENALTY=6.736909 PRIMER_LEFT_138_PENALTY=2.137489 PRIMER_RIGHT_138_PENALTY=4.599420 PRIMER_LEFT_138_SEQUENCE=GGTACCCACTGATGGTAATGCT PRIMER_RIGHT_138_SEQUENCE=CTCTTCTCACTGCATGTCTCTTTG PRIMER_LEFT_138=256,22 PRIMER_RIGHT_138=684,24 PRIMER_LEFT_138_TM=60.137 PRIMER_RIGHT_138_TM=60.599 PRIMER_LEFT_138_GC_PERCENT=50.000 PRIMER_RIGHT_138_GC_PERCENT=45.833 PRIMER_LEFT_138_SELF_ANY=6.00 PRIMER_RIGHT_138_SELF_ANY=4.00 PRIMER_LEFT_138_SELF_END=0.00 PRIMER_RIGHT_138_SELF_END=0.00 PRIMER_LEFT_138_END_STABILITY=8.1000 PRIMER_RIGHT_138_END_STABILITY=7.3000 PRIMER_PAIR_138_COMPL_ANY=4.00 PRIMER_PAIR_138_COMPL_END=0.00 PRIMER_PAIR_138_PRODUCT_SIZE=429 PRIMER_PAIR_139_PENALTY=6.768315 PRIMER_LEFT_139_PENALTY=2.150273 PRIMER_RIGHT_139_PENALTY=4.618042 PRIMER_LEFT_139_SEQUENCE=CCCCAGATTGCCATGTTC PRIMER_RIGHT_139_SEQUENCE=TACTTTGTCTTCACTCCCATCTG PRIMER_LEFT_139=293,18 PRIMER_RIGHT_139=874,23 PRIMER_LEFT_139_TM=59.850 PRIMER_RIGHT_139_TM=58.382 PRIMER_LEFT_139_GC_PERCENT=55.556 PRIMER_RIGHT_139_GC_PERCENT=43.478 PRIMER_LEFT_139_SELF_ANY=4.00 PRIMER_RIGHT_139_SELF_ANY=2.00 PRIMER_LEFT_139_SELF_END=0.00 PRIMER_RIGHT_139_SELF_END=2.00 PRIMER_LEFT_139_END_STABILITY=6.7000 PRIMER_RIGHT_139_END_STABILITY=6.6000 PRIMER_PAIR_139_COMPL_ANY=5.00 PRIMER_PAIR_139_COMPL_END=1.00 PRIMER_PAIR_139_PRODUCT_SIZE=582 PRIMER_PAIR_140_PENALTY=6.800533 PRIMER_LEFT_140_PENALTY=6.438766 PRIMER_RIGHT_140_PENALTY=0.361767 PRIMER_LEFT_140_SEQUENCE=GCAGTATTGCCAAGAAGTCTACCC PRIMER_RIGHT_140_SEQUENCE=CTCGTCATCATCGGCTTCTT PRIMER_LEFT_140=409,24 PRIMER_RIGHT_140=937,20 PRIMER_LEFT_140_TM=62.439 PRIMER_RIGHT_140_TM=60.362 PRIMER_LEFT_140_GC_PERCENT=50.000 PRIMER_RIGHT_140_GC_PERCENT=50.000 PRIMER_LEFT_140_SELF_ANY=6.00 PRIMER_RIGHT_140_SELF_ANY=3.00 PRIMER_LEFT_140_SELF_END=0.00 PRIMER_RIGHT_140_SELF_END=0.00 PRIMER_LEFT_140_END_STABILITY=8.4000 PRIMER_RIGHT_140_END_STABILITY=7.0000 PRIMER_PAIR_140_COMPL_ANY=6.00 PRIMER_PAIR_140_COMPL_END=3.00 PRIMER_PAIR_140_PRODUCT_SIZE=529 PRIMER_PAIR_141_PENALTY=6.810499 PRIMER_LEFT_141_PENALTY=2.151267 PRIMER_RIGHT_141_PENALTY=4.659232 PRIMER_LEFT_141_SEQUENCE=AGTGGGATTCAGATCCATCA PRIMER_RIGHT_141_SEQUENCE=CTACTTTGTCTTCACTCCCATCTG PRIMER_LEFT_141=351,20 PRIMER_RIGHT_141=875,24 PRIMER_LEFT_141_TM=57.849 PRIMER_RIGHT_141_TM=59.341 PRIMER_LEFT_141_GC_PERCENT=45.000 PRIMER_RIGHT_141_GC_PERCENT=45.833 PRIMER_LEFT_141_SELF_ANY=5.00 PRIMER_RIGHT_141_SELF_ANY=2.00 PRIMER_LEFT_141_SELF_END=2.00 PRIMER_RIGHT_141_SELF_END=2.00 PRIMER_LEFT_141_END_STABILITY=6.9000 PRIMER_RIGHT_141_END_STABILITY=6.6000 PRIMER_PAIR_141_COMPL_ANY=5.00 PRIMER_PAIR_141_COMPL_END=2.00 PRIMER_PAIR_141_PRODUCT_SIZE=525 PRIMER_PAIR_142_PENALTY=6.888757 PRIMER_LEFT_142_PENALTY=3.362783 PRIMER_RIGHT_142_PENALTY=3.525975 PRIMER_LEFT_142_SEQUENCE=CTCGGGCTCCGTCAGTTT PRIMER_RIGHT_142_SEQUENCE=TTACCATCAGTGGGTACCTCCA PRIMER_LEFT_142=40,18 PRIMER_RIGHT_142=273,22 PRIMER_LEFT_142_TM=61.363 PRIMER_RIGHT_142_TM=61.526 PRIMER_LEFT_142_GC_PERCENT=61.111 PRIMER_RIGHT_142_GC_PERCENT=50.000 PRIMER_LEFT_142_SELF_ANY=4.00 PRIMER_RIGHT_142_SELF_ANY=6.00 PRIMER_LEFT_142_SELF_END=0.00 PRIMER_RIGHT_142_SELF_END=2.00 PRIMER_LEFT_142_END_STABILITY=6.7000 PRIMER_RIGHT_142_END_STABILITY=8.2000 PRIMER_PAIR_142_COMPL_ANY=2.00 PRIMER_PAIR_142_COMPL_END=1.00 PRIMER_PAIR_142_PRODUCT_SIZE=234 PRIMER_PAIR_143_PENALTY=6.961248 PRIMER_LEFT_143_PENALTY=2.137489 PRIMER_RIGHT_143_PENALTY=4.823759 PRIMER_LEFT_143_SEQUENCE=AGGTACCCACTGATGGTAATGC PRIMER_RIGHT_143_SEQUENCE=TCTCACTGCATGTCTCTTTGGC PRIMER_LEFT_143=255,22 PRIMER_RIGHT_143=680,22 PRIMER_LEFT_143_TM=60.137 PRIMER_RIGHT_143_TM=62.824 PRIMER_LEFT_143_GC_PERCENT=50.000 PRIMER_RIGHT_143_GC_PERCENT=50.000 PRIMER_LEFT_143_SELF_ANY=6.00 PRIMER_RIGHT_143_SELF_ANY=4.00 PRIMER_LEFT_143_SELF_END=2.00 PRIMER_RIGHT_143_SELF_END=2.00 PRIMER_LEFT_143_END_STABILITY=8.4000 PRIMER_RIGHT_143_END_STABILITY=10.0000 PRIMER_PAIR_143_COMPL_ANY=4.00 PRIMER_PAIR_143_COMPL_END=2.00 PRIMER_PAIR_143_PRODUCT_SIZE=426 PRIMER_PAIR_144_PENALTY=6.975656 PRIMER_LEFT_144_PENALTY=6.610485 PRIMER_RIGHT_144_PENALTY=0.365170 PRIMER_LEFT_144_SEQUENCE=TTGCCAAGAAGTCTACCCTGAACT PRIMER_RIGHT_144_SEQUENCE=CGTAAAAGAATGGGGCACAC PRIMER_LEFT_144=415,24 PRIMER_RIGHT_144=1163,20 PRIMER_LEFT_144_TM=62.610 PRIMER_RIGHT_144_TM=60.365 PRIMER_LEFT_144_GC_PERCENT=45.833 PRIMER_RIGHT_144_GC_PERCENT=50.000 PRIMER_LEFT_144_SELF_ANY=5.00 PRIMER_RIGHT_144_SELF_ANY=3.00 PRIMER_LEFT_144_SELF_END=1.00 PRIMER_RIGHT_144_SELF_END=1.00 PRIMER_LEFT_144_END_STABILITY=6.4000 PRIMER_RIGHT_144_END_STABILITY=6.4000 PRIMER_PAIR_144_COMPL_ANY=4.00 PRIMER_PAIR_144_COMPL_END=2.00 PRIMER_PAIR_144_PRODUCT_SIZE=749 PRIMER_PAIR_145_PENALTY=6.992001 PRIMER_LEFT_145_PENALTY=2.150151 PRIMER_RIGHT_145_PENALTY=4.841851 PRIMER_LEFT_145_SEQUENCE=ATGAATGTCCAGAATGGGAAG PRIMER_RIGHT_145_SEQUENCE=AAACTCACCAACTAAGCAGCGG PRIMER_LEFT_145=332,21 PRIMER_RIGHT_145=565,22 PRIMER_LEFT_145_TM=58.850 PRIMER_RIGHT_145_TM=62.842 PRIMER_LEFT_145_GC_PERCENT=42.857 PRIMER_RIGHT_145_GC_PERCENT=50.000 PRIMER_LEFT_145_SELF_ANY=3.00 PRIMER_RIGHT_145_SELF_ANY=3.00 PRIMER_LEFT_145_SELF_END=0.00 PRIMER_RIGHT_145_SELF_END=2.00 PRIMER_LEFT_145_END_STABILITY=8.2000 PRIMER_RIGHT_145_END_STABILITY=11.4000 PRIMER_PAIR_145_COMPL_ANY=3.00 PRIMER_PAIR_145_COMPL_END=0.00 PRIMER_PAIR_145_PRODUCT_SIZE=234 PRIMER_PAIR_146_PENALTY=7.015504 PRIMER_LEFT_146_PENALTY=6.638755 PRIMER_RIGHT_146_PENALTY=0.376749 PRIMER_LEFT_146_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_146_SEQUENCE=CTCTCGAACCACCTCTTCCA PRIMER_LEFT_146=411,25 PRIMER_RIGHT_146=1066,20 PRIMER_LEFT_146_TM=61.639 PRIMER_RIGHT_146_TM=60.377 PRIMER_LEFT_146_GC_PERCENT=44.000 PRIMER_RIGHT_146_GC_PERCENT=55.000 PRIMER_LEFT_146_SELF_ANY=5.00 PRIMER_RIGHT_146_SELF_ANY=4.00 PRIMER_LEFT_146_SELF_END=1.00 PRIMER_RIGHT_146_SELF_END=0.00 PRIMER_LEFT_146_END_STABILITY=8.2000 PRIMER_RIGHT_146_END_STABILITY=8.5000 PRIMER_PAIR_146_COMPL_ANY=5.00 PRIMER_PAIR_146_COMPL_END=1.00 PRIMER_PAIR_146_PRODUCT_SIZE=656 PRIMER_PAIR_147_PENALTY=7.063424 PRIMER_LEFT_147_PENALTY=2.171836 PRIMER_RIGHT_147_PENALTY=4.891588 PRIMER_LEFT_147_SEQUENCE=CACATGAATGTCCAGAATGGG PRIMER_RIGHT_147_SEQUENCE=CACACCTCTCGAACCACCTCTT PRIMER_LEFT_147=329,21 PRIMER_RIGHT_147=1071,22 PRIMER_LEFT_147_TM=61.172 PRIMER_RIGHT_147_TM=62.892 PRIMER_LEFT_147_GC_PERCENT=47.619 PRIMER_RIGHT_147_GC_PERCENT=54.545 PRIMER_LEFT_147_SELF_ANY=6.00 PRIMER_RIGHT_147_SELF_ANY=4.00 PRIMER_LEFT_147_SELF_END=1.00 PRIMER_RIGHT_147_SELF_END=0.00 PRIMER_LEFT_147_END_STABILITY=9.6000 PRIMER_RIGHT_147_END_STABILITY=6.7000 PRIMER_PAIR_147_COMPL_ANY=4.00 PRIMER_PAIR_147_COMPL_END=2.00 PRIMER_PAIR_147_PRODUCT_SIZE=743 PRIMER_PAIR_148_PENALTY=7.093953 PRIMER_LEFT_148_PENALTY=2.174817 PRIMER_RIGHT_148_PENALTY=4.919136 PRIMER_LEFT_148_SEQUENCE=ACCAAAACCTGCATTGATACC PRIMER_RIGHT_148_SEQUENCE=ACTTTGTCTTCACTCCCATCTGC PRIMER_LEFT_148=374,21 PRIMER_RIGHT_148=873,23 PRIMER_LEFT_148_TM=58.825 PRIMER_RIGHT_148_TM=61.919 PRIMER_LEFT_148_GC_PERCENT=42.857 PRIMER_RIGHT_148_GC_PERCENT=47.826 PRIMER_LEFT_148_SELF_ANY=4.00 PRIMER_RIGHT_148_SELF_ANY=2.00 PRIMER_LEFT_148_SELF_END=2.00 PRIMER_RIGHT_148_SELF_END=2.00 PRIMER_LEFT_148_END_STABILITY=6.8000 PRIMER_RIGHT_148_END_STABILITY=8.2000 PRIMER_PAIR_148_COMPL_ANY=4.00 PRIMER_PAIR_148_COMPL_END=0.00 PRIMER_PAIR_148_PRODUCT_SIZE=500 PRIMER_PAIR_149_PENALTY=7.107233 PRIMER_LEFT_149_PENALTY=2.186531 PRIMER_RIGHT_149_PENALTY=4.920702 PRIMER_LEFT_149_SEQUENCE=AGGGACCAAAACCTGCATTG PRIMER_RIGHT_149_SEQUENCE=GAGTAAACTTTGGGACATGGCG PRIMER_LEFT_149=370,20 PRIMER_RIGHT_149=1249,22 PRIMER_LEFT_149_TM=62.187 PRIMER_RIGHT_149_TM=62.921 PRIMER_LEFT_149_GC_PERCENT=50.000 PRIMER_RIGHT_149_GC_PERCENT=50.000 PRIMER_LEFT_149_SELF_ANY=4.00 PRIMER_RIGHT_149_SELF_ANY=5.00 PRIMER_LEFT_149_SELF_END=3.00 PRIMER_RIGHT_149_SELF_END=2.00 PRIMER_LEFT_149_END_STABILITY=7.2000 PRIMER_RIGHT_149_END_STABILITY=11.7000 PRIMER_PAIR_149_COMPL_ANY=5.00 PRIMER_PAIR_149_COMPL_END=1.00 PRIMER_PAIR_149_PRODUCT_SIZE=880 PRIMER_PAIR_150_PENALTY=7.187746 PRIMER_LEFT_150_PENALTY=2.186531 PRIMER_RIGHT_150_PENALTY=5.001215 PRIMER_LEFT_150_SEQUENCE=CAGGGACCAAAACCTGCATT PRIMER_RIGHT_150_SEQUENCE=CTGGCTGCTGTTGTAGGAAGTTT PRIMER_LEFT_150=369,20 PRIMER_RIGHT_150=1308,23 PRIMER_LEFT_150_TM=62.187 PRIMER_RIGHT_150_TM=62.001 PRIMER_LEFT_150_GC_PERCENT=50.000 PRIMER_RIGHT_150_GC_PERCENT=47.826 PRIMER_LEFT_150_SELF_ANY=4.00 PRIMER_RIGHT_150_SELF_ANY=3.00 PRIMER_LEFT_150_SELF_END=1.00 PRIMER_RIGHT_150_SELF_END=2.00 PRIMER_LEFT_150_END_STABILITY=8.4000 PRIMER_RIGHT_150_END_STABILITY=6.7000 PRIMER_PAIR_150_COMPL_ANY=4.00 PRIMER_PAIR_150_COMPL_END=2.00 PRIMER_PAIR_150_PRODUCT_SIZE=940 PRIMER_PAIR_151_PENALTY=7.220368 PRIMER_LEFT_151_PENALTY=2.203644 PRIMER_RIGHT_151_PENALTY=5.016724 PRIMER_LEFT_151_SEQUENCE=CTGGAGGTACCCACTGATGGT PRIMER_RIGHT_151_SEQUENCE=ACTCACCAACTAAGCAGCGGTAG PRIMER_LEFT_151=251,21 PRIMER_RIGHT_151=563,23 PRIMER_LEFT_151_TM=61.204 PRIMER_RIGHT_151_TM=62.017 PRIMER_LEFT_151_GC_PERCENT=57.143 PRIMER_RIGHT_151_GC_PERCENT=52.174 PRIMER_LEFT_151_SELF_ANY=6.00 PRIMER_RIGHT_151_SELF_ANY=3.00 PRIMER_LEFT_151_SELF_END=3.00 PRIMER_RIGHT_151_SELF_END=3.00 PRIMER_LEFT_151_END_STABILITY=7.8000 PRIMER_RIGHT_151_END_STABILITY=6.9000 PRIMER_PAIR_151_COMPL_ANY=4.00 PRIMER_PAIR_151_COMPL_END=3.00 PRIMER_PAIR_151_PRODUCT_SIZE=313 PRIMER_PAIR_152_PENALTY=7.271327 PRIMER_LEFT_152_PENALTY=2.207202 PRIMER_RIGHT_152_PENALTY=5.064124 PRIMER_LEFT_152_SEQUENCE=GAATGGGAAGTGGGATTCAGA PRIMER_RIGHT_152_SEQUENCE=TTGTAGGAAGTTTAACAGGATCTCG PRIMER_LEFT_152=343,21 PRIMER_RIGHT_152=1298,25 PRIMER_LEFT_152_TM=61.207 PRIMER_RIGHT_152_TM=60.064 PRIMER_LEFT_152_GC_PERCENT=47.619 PRIMER_RIGHT_152_GC_PERCENT=40.000 PRIMER_LEFT_152_SELF_ANY=4.00 PRIMER_RIGHT_152_SELF_ANY=5.00 PRIMER_LEFT_152_SELF_END=3.00 PRIMER_RIGHT_152_SELF_END=2.00 PRIMER_LEFT_152_END_STABILITY=6.7000 PRIMER_RIGHT_152_END_STABILITY=8.4000 PRIMER_PAIR_152_COMPL_ANY=3.00 PRIMER_PAIR_152_COMPL_END=2.00 PRIMER_PAIR_152_PRODUCT_SIZE=956 PRIMER_PAIR_153_PENALTY=7.293981 PRIMER_LEFT_153_PENALTY=2.225062 PRIMER_RIGHT_153_PENALTY=5.068920 PRIMER_LEFT_153_SEQUENCE=ATGAATGTCCAGAATGGGAA PRIMER_RIGHT_153_SEQUENCE=GCTGTTGTAGGAAGTTTAACAGGA PRIMER_LEFT_153=332,20 PRIMER_RIGHT_153=1302,24 PRIMER_LEFT_153_TM=57.775 PRIMER_RIGHT_153_TM=58.931 PRIMER_LEFT_153_GC_PERCENT=40.000 PRIMER_RIGHT_153_GC_PERCENT=41.667 PRIMER_LEFT_153_SELF_ANY=3.00 PRIMER_RIGHT_153_SELF_ANY=5.00 PRIMER_LEFT_153_SELF_END=1.00 PRIMER_RIGHT_153_SELF_END=0.00 PRIMER_LEFT_153_END_STABILITY=9.7000 PRIMER_RIGHT_153_END_STABILITY=8.2000 PRIMER_PAIR_153_COMPL_ANY=3.00 PRIMER_PAIR_153_COMPL_END=0.00 PRIMER_PAIR_153_PRODUCT_SIZE=971 PRIMER_PAIR_154_PENALTY=7.330509 PRIMER_LEFT_154_PENALTY=6.944753 PRIMER_RIGHT_154_PENALTY=0.385756 PRIMER_LEFT_154_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_154_SEQUENCE=TCCACCTCAGCCACTTCTTC PRIMER_LEFT_154=413,25 PRIMER_RIGHT_154=912,20 PRIMER_LEFT_154_TM=61.945 PRIMER_RIGHT_154_TM=60.386 PRIMER_LEFT_154_GC_PERCENT=44.000 PRIMER_RIGHT_154_GC_PERCENT=55.000 PRIMER_LEFT_154_SELF_ANY=5.00 PRIMER_RIGHT_154_SELF_ANY=3.00 PRIMER_LEFT_154_SELF_END=0.00 PRIMER_RIGHT_154_SELF_END=0.00 PRIMER_LEFT_154_END_STABILITY=6.7000 PRIMER_RIGHT_154_END_STABILITY=6.7000 PRIMER_PAIR_154_COMPL_ANY=7.00 PRIMER_PAIR_154_COMPL_END=2.00 PRIMER_PAIR_154_PRODUCT_SIZE=500 PRIMER_PAIR_155_PENALTY=7.330509 PRIMER_LEFT_155_PENALTY=6.944753 PRIMER_RIGHT_155_PENALTY=0.385756 PRIMER_LEFT_155_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_155_SEQUENCE=TCTTCCACCTCAGCCACTTC PRIMER_LEFT_155=412,25 PRIMER_RIGHT_155=915,20 PRIMER_LEFT_155_TM=61.945 PRIMER_RIGHT_155_TM=60.386 PRIMER_LEFT_155_GC_PERCENT=44.000 PRIMER_RIGHT_155_GC_PERCENT=55.000 PRIMER_LEFT_155_SELF_ANY=5.00 PRIMER_RIGHT_155_SELF_ANY=3.00 PRIMER_LEFT_155_SELF_END=0.00 PRIMER_RIGHT_155_SELF_END=0.00 PRIMER_LEFT_155_END_STABILITY=7.0000 PRIMER_RIGHT_155_END_STABILITY=6.4000 PRIMER_PAIR_155_COMPL_ANY=5.00 PRIMER_PAIR_155_COMPL_END=3.00 PRIMER_PAIR_155_PRODUCT_SIZE=504 PRIMER_PAIR_156_PENALTY=7.336644 PRIMER_LEFT_156_PENALTY=2.222066 PRIMER_RIGHT_156_PENALTY=5.114578 PRIMER_LEFT_156_SEQUENCE=GGAGGTACCCACTGATGGTAA PRIMER_RIGHT_156_SEQUENCE=CTCTTCTCACTGCATGTCTCTTT PRIMER_LEFT_156=253,21 PRIMER_RIGHT_156=684,23 PRIMER_LEFT_156_TM=58.778 PRIMER_RIGHT_156_TM=57.885 PRIMER_LEFT_156_GC_PERCENT=52.381 PRIMER_RIGHT_156_GC_PERCENT=43.478 PRIMER_LEFT_156_SELF_ANY=6.00 PRIMER_RIGHT_156_SELF_ANY=4.00 PRIMER_LEFT_156_SELF_END=3.00 PRIMER_RIGHT_156_SELF_END=0.00 PRIMER_LEFT_156_END_STABILITY=7.2000 PRIMER_RIGHT_156_END_STABILITY=7.0000 PRIMER_PAIR_156_COMPL_ANY=4.00 PRIMER_PAIR_156_COMPL_END=2.00 PRIMER_PAIR_156_PRODUCT_SIZE=432 PRIMER_PAIR_157_PENALTY=7.340305 PRIMER_LEFT_157_PENALTY=2.227266 PRIMER_RIGHT_157_PENALTY=5.113039 PRIMER_LEFT_157_SEQUENCE=GTCCAGAATGGGAAGTGGGA PRIMER_RIGHT_157_SEQUENCE=TGTAGGAAGTTTAACAGGATCTCG PRIMER_LEFT_157=338,20 PRIMER_RIGHT_157=1297,24 PRIMER_LEFT_157_TM=62.227 PRIMER_RIGHT_157_TM=58.887 PRIMER_LEFT_157_GC_PERCENT=55.000 PRIMER_RIGHT_157_GC_PERCENT=41.667 PRIMER_LEFT_157_SELF_ANY=3.00 PRIMER_RIGHT_157_SELF_ANY=5.00 PRIMER_LEFT_157_SELF_END=0.00 PRIMER_RIGHT_157_SELF_END=2.00 PRIMER_LEFT_157_END_STABILITY=9.7000 PRIMER_RIGHT_157_END_STABILITY=8.4000 PRIMER_PAIR_157_COMPL_ANY=3.00 PRIMER_PAIR_157_COMPL_END=1.00 PRIMER_PAIR_157_PRODUCT_SIZE=960 PRIMER_PAIR_158_PENALTY=7.394664 PRIMER_LEFT_158_PENALTY=2.292791 PRIMER_RIGHT_158_PENALTY=5.101873 PRIMER_LEFT_158_SEQUENCE=GGGACCAAAACCTGCATTG PRIMER_RIGHT_158_SEQUENCE=ACTACTTTGTCTTCACTCCCATCTG PRIMER_LEFT_158=371,19 PRIMER_RIGHT_158=876,25 PRIMER_LEFT_158_TM=61.293 PRIMER_RIGHT_158_TM=60.102 PRIMER_LEFT_158_GC_PERCENT=52.632 PRIMER_RIGHT_158_GC_PERCENT=44.000 PRIMER_LEFT_158_SELF_ANY=4.00 PRIMER_RIGHT_158_SELF_ANY=2.00 PRIMER_LEFT_158_SELF_END=3.00 PRIMER_RIGHT_158_SELF_END=2.00 PRIMER_LEFT_158_END_STABILITY=7.2000 PRIMER_RIGHT_158_END_STABILITY=6.6000 PRIMER_PAIR_158_COMPL_ANY=4.00 PRIMER_PAIR_158_COMPL_END=1.00 PRIMER_PAIR_158_PRODUCT_SIZE=506 PRIMER_PAIR_159_PENALTY=7.474704 PRIMER_LEFT_159_PENALTY=3.683571 PRIMER_RIGHT_159_PENALTY=3.791133 PRIMER_LEFT_159_SEQUENCE=AGCGGTAGGCGAGAGCAC PRIMER_RIGHT_159_SEQUENCE=CAGGGTAGACTTCTTGGCAATAC PRIMER_LEFT_159=65,18 PRIMER_RIGHT_159=434,23 PRIMER_LEFT_159_TM=61.684 PRIMER_RIGHT_159_TM=59.209 PRIMER_LEFT_159_GC_PERCENT=66.667 PRIMER_RIGHT_159_GC_PERCENT=47.826 PRIMER_LEFT_159_SELF_ANY=2.00 PRIMER_RIGHT_159_SELF_ANY=5.00 PRIMER_LEFT_159_SELF_END=2.00 PRIMER_RIGHT_159_SELF_END=3.00 PRIMER_LEFT_159_END_STABILITY=7.9000 PRIMER_RIGHT_159_END_STABILITY=5.6000 PRIMER_PAIR_159_COMPL_ANY=3.00 PRIMER_PAIR_159_COMPL_END=1.00 PRIMER_PAIR_159_PRODUCT_SIZE=370 PRIMER_PAIR_160_PENALTY=7.497858 PRIMER_LEFT_160_PENALTY=2.303792 PRIMER_RIGHT_160_PENALTY=5.194065 PRIMER_LEFT_160_SEQUENCE=ATGAATGTCCAGAATGGGAAGT PRIMER_RIGHT_160_SEQUENCE=CACTTACAAACTCACCAACTAAGCA PRIMER_LEFT_160=332,22 PRIMER_RIGHT_160=572,25 PRIMER_LEFT_160_TM=59.696 PRIMER_RIGHT_160_TM=59.806 PRIMER_LEFT_160_GC_PERCENT=40.909 PRIMER_RIGHT_160_GC_PERCENT=40.000 PRIMER_LEFT_160_SELF_ANY=3.00 PRIMER_RIGHT_160_SELF_ANY=4.00 PRIMER_LEFT_160_SELF_END=1.00 PRIMER_RIGHT_160_SELF_END=0.00 PRIMER_LEFT_160_END_STABILITY=6.4000 PRIMER_RIGHT_160_END_STABILITY=8.5000 PRIMER_PAIR_160_COMPL_ANY=4.00 PRIMER_PAIR_160_COMPL_END=2.00 PRIMER_PAIR_160_PRODUCT_SIZE=241 PRIMER_PAIR_161_PENALTY=7.534720 PRIMER_LEFT_161_PENALTY=3.708825 PRIMER_RIGHT_161_PENALTY=3.825895 PRIMER_LEFT_161_SEQUENCE=GGCTCCGTCAGTTTCCTC PRIMER_RIGHT_161_SEQUENCE=TGCAGTTCAGGGTAGACTTCTTG PRIMER_LEFT_161=44,18 PRIMER_RIGHT_161=441,23 PRIMER_LEFT_161_TM=58.291 PRIMER_RIGHT_161_TM=60.826 PRIMER_LEFT_161_GC_PERCENT=61.111 PRIMER_RIGHT_161_GC_PERCENT=47.826 PRIMER_LEFT_161_SELF_ANY=2.00 PRIMER_RIGHT_161_SELF_ANY=4.00 PRIMER_LEFT_161_SELF_END=0.00 PRIMER_RIGHT_161_SELF_END=1.00 PRIMER_LEFT_161_END_STABILITY=7.9000 PRIMER_RIGHT_161_END_STABILITY=7.0000 PRIMER_PAIR_161_COMPL_ANY=3.00 PRIMER_PAIR_161_COMPL_END=1.00 PRIMER_PAIR_161_PRODUCT_SIZE=398 PRIMER_PAIR_162_PENALTY=7.571660 PRIMER_LEFT_162_PENALTY=2.315468 PRIMER_RIGHT_162_PENALTY=5.256192 PRIMER_LEFT_162_SEQUENCE=CTGATGGTAATGCTGGCCT PRIMER_RIGHT_162_SEQUENCE=ACTCTTCTCACTGCATGTCTCTTT PRIMER_LEFT_162=264,19 PRIMER_RIGHT_162=685,24 PRIMER_LEFT_162_TM=58.685 PRIMER_RIGHT_162_TM=58.744 PRIMER_LEFT_162_GC_PERCENT=52.632 PRIMER_RIGHT_162_GC_PERCENT=41.667 PRIMER_LEFT_162_SELF_ANY=4.00 PRIMER_RIGHT_162_SELF_ANY=4.00 PRIMER_LEFT_162_SELF_END=2.00 PRIMER_RIGHT_162_SELF_END=0.00 PRIMER_LEFT_162_END_STABILITY=10.9000 PRIMER_RIGHT_162_END_STABILITY=7.0000 PRIMER_PAIR_162_COMPL_ANY=4.00 PRIMER_PAIR_162_COMPL_END=0.00 PRIMER_PAIR_162_PRODUCT_SIZE=422 PRIMER_PAIR_163_PENALTY=7.656291 PRIMER_LEFT_163_PENALTY=3.791523 PRIMER_RIGHT_163_PENALTY=3.864768 PRIMER_LEFT_163_SEQUENCE=AGCTGACTCGCCTGGCTCT PRIMER_RIGHT_163_SEQUENCE=GCAGTTCAGGGTAGACTTCTTG PRIMER_LEFT_163=5,19 PRIMER_RIGHT_163=440,22 PRIMER_LEFT_163_TM=62.792 PRIMER_RIGHT_163_TM=58.135 PRIMER_LEFT_163_GC_PERCENT=63.158 PRIMER_RIGHT_163_GC_PERCENT=50.000 PRIMER_LEFT_163_SELF_ANY=5.00 PRIMER_RIGHT_163_SELF_ANY=4.00 PRIMER_LEFT_163_SELF_END=0.00 PRIMER_RIGHT_163_SELF_END=1.00 PRIMER_LEFT_163_END_STABILITY=7.9000 PRIMER_RIGHT_163_END_STABILITY=7.0000 PRIMER_PAIR_163_COMPL_ANY=4.00 PRIMER_PAIR_163_COMPL_END=2.00 PRIMER_PAIR_163_PRODUCT_SIZE=436 PRIMER_PAIR_164_PENALTY=7.663983 PRIMER_LEFT_164_PENALTY=2.325240 PRIMER_RIGHT_164_PENALTY=5.338743 PRIMER_LEFT_164_SEQUENCE=GCACATGAATGTCCAGAATGG PRIMER_RIGHT_164_SEQUENCE=AACTCACCAACTAAGCAGCGGTA PRIMER_LEFT_164=328,21 PRIMER_RIGHT_164=564,23 PRIMER_LEFT_164_TM=61.325 PRIMER_RIGHT_164_TM=62.339 PRIMER_LEFT_164_GC_PERCENT=47.619 PRIMER_RIGHT_164_GC_PERCENT=47.826 PRIMER_LEFT_164_SELF_ANY=6.00 PRIMER_RIGHT_164_SELF_ANY=3.00 PRIMER_LEFT_164_SELF_END=3.00 PRIMER_RIGHT_164_SELF_END=2.00 PRIMER_LEFT_164_END_STABILITY=8.4000 PRIMER_RIGHT_164_END_STABILITY=8.9000 PRIMER_PAIR_164_COMPL_ANY=3.00 PRIMER_PAIR_164_COMPL_END=0.00 PRIMER_PAIR_164_PRODUCT_SIZE=237 PRIMER_PAIR_165_PENALTY=7.738336 PRIMER_LEFT_165_PENALTY=3.873568 PRIMER_RIGHT_165_PENALTY=3.864768 PRIMER_LEFT_165_SEQUENCE=GCTGACTCGCCTGGCTCT PRIMER_RIGHT_165_SEQUENCE=TGCAGTTCAGGGTAGACTTCTT PRIMER_LEFT_165=6,18 PRIMER_RIGHT_165=441,22 PRIMER_LEFT_165_TM=61.874 PRIMER_RIGHT_165_TM=58.135 PRIMER_LEFT_165_GC_PERCENT=66.667 PRIMER_RIGHT_165_GC_PERCENT=45.455 PRIMER_LEFT_165_SELF_ANY=5.00 PRIMER_RIGHT_165_SELF_ANY=4.00 PRIMER_LEFT_165_SELF_END=0.00 PRIMER_RIGHT_165_SELF_END=2.00 PRIMER_LEFT_165_END_STABILITY=7.9000 PRIMER_RIGHT_165_END_STABILITY=7.0000 PRIMER_PAIR_165_COMPL_ANY=4.00 PRIMER_PAIR_165_COMPL_END=1.00 PRIMER_PAIR_165_PRODUCT_SIZE=436 PRIMER_PAIR_166_PENALTY=7.741586 PRIMER_LEFT_166_PENALTY=2.376315 PRIMER_RIGHT_166_PENALTY=5.365271 PRIMER_LEFT_166_SEQUENCE=GGTAATGCTGGCCTGCTG PRIMER_RIGHT_166_SEQUENCE=GGTACTCTTCTCACTGCATGTCTCT PRIMER_LEFT_166=269,18 PRIMER_RIGHT_166=688,25 PRIMER_LEFT_166_TM=60.376 PRIMER_RIGHT_166_TM=60.365 PRIMER_LEFT_166_GC_PERCENT=61.111 PRIMER_RIGHT_166_GC_PERCENT=48.000 PRIMER_LEFT_166_SELF_ANY=6.00 PRIMER_RIGHT_166_SELF_ANY=4.00 PRIMER_LEFT_166_SELF_END=2.00 PRIMER_RIGHT_166_SELF_END=0.00 PRIMER_LEFT_166_END_STABILITY=8.5000 PRIMER_RIGHT_166_END_STABILITY=6.4000 PRIMER_PAIR_166_COMPL_ANY=4.00 PRIMER_PAIR_166_COMPL_END=0.00 PRIMER_PAIR_166_PRODUCT_SIZE=420 PRIMER_PAIR_167_PENALTY=7.762954 PRIMER_LEFT_167_PENALTY=3.873568 PRIMER_RIGHT_167_PENALTY=3.889386 PRIMER_LEFT_167_SEQUENCE=AGCTGACTCGCCTGGCTC PRIMER_RIGHT_167_SEQUENCE=CTGCAGTTCAGGGTAGACTTCTT PRIMER_LEFT_167=5,18 PRIMER_RIGHT_167=442,23 PRIMER_LEFT_167_TM=61.874 PRIMER_RIGHT_167_TM=59.111 PRIMER_LEFT_167_GC_PERCENT=66.667 PRIMER_RIGHT_167_GC_PERCENT=47.826 PRIMER_LEFT_167_SELF_ANY=5.00 PRIMER_RIGHT_167_SELF_ANY=6.00 PRIMER_LEFT_167_SELF_END=1.00 PRIMER_RIGHT_167_SELF_END=2.00 PRIMER_LEFT_167_END_STABILITY=9.4000 PRIMER_RIGHT_167_END_STABILITY=7.0000 PRIMER_PAIR_167_COMPL_ANY=4.00 PRIMER_PAIR_167_COMPL_END=2.00 PRIMER_PAIR_167_PRODUCT_SIZE=438 PRIMER_PAIR_168_PENALTY=7.783163 PRIMER_LEFT_168_PENALTY=2.389824 PRIMER_RIGHT_168_PENALTY=5.393340 PRIMER_LEFT_168_SEQUENCE=TTGATACCAAGGAAGGCATC PRIMER_RIGHT_168_SEQUENCE=GTCTTCACTCCCATCTGCATAGTC PRIMER_LEFT_168=387,20 PRIMER_RIGHT_168=868,24 PRIMER_LEFT_168_TM=57.610 PRIMER_RIGHT_168_TM=61.393 PRIMER_LEFT_168_GC_PERCENT=45.000 PRIMER_RIGHT_168_GC_PERCENT=50.000 PRIMER_LEFT_168_SELF_ANY=6.00 PRIMER_RIGHT_168_SELF_ANY=4.00 PRIMER_LEFT_168_SELF_END=3.00 PRIMER_RIGHT_168_SELF_END=1.00 PRIMER_LEFT_168_END_STABILITY=8.1000 PRIMER_RIGHT_168_END_STABILITY=5.4000 PRIMER_PAIR_168_COMPL_ANY=4.00 PRIMER_PAIR_168_COMPL_END=0.00 PRIMER_PAIR_168_PRODUCT_SIZE=482 PRIMER_PAIR_169_PENALTY=7.823941 PRIMER_LEFT_169_PENALTY=7.438185 PRIMER_RIGHT_169_PENALTY=0.385756 PRIMER_LEFT_169_SEQUENCE=CAGTATTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_169_SEQUENCE=TCTTCTTCCACCTCAGCCAC PRIMER_LEFT_169=410,25 PRIMER_RIGHT_169=918,20 PRIMER_LEFT_169_TM=62.438 PRIMER_RIGHT_169_TM=60.386 PRIMER_LEFT_169_GC_PERCENT=48.000 PRIMER_RIGHT_169_GC_PERCENT=55.000 PRIMER_LEFT_169_SELF_ANY=5.00 PRIMER_RIGHT_169_SELF_ANY=3.00 PRIMER_LEFT_169_SELF_END=1.00 PRIMER_RIGHT_169_SELF_END=0.00 PRIMER_LEFT_169_END_STABILITY=9.7000 PRIMER_RIGHT_169_END_STABILITY=9.4000 PRIMER_PAIR_169_COMPL_ANY=6.00 PRIMER_PAIR_169_COMPL_END=1.00 PRIMER_PAIR_169_PRODUCT_SIZE=509 PRIMER_PAIR_170_PENALTY=7.911309 PRIMER_LEFT_170_PENALTY=3.902978 PRIMER_RIGHT_170_PENALTY=4.008330 PRIMER_LEFT_170_SEQUENCE=CTGACTCGCCTGGCTCTGA PRIMER_RIGHT_170_SEQUENCE=CAGTTCAGGGTAGACTTCTTGG PRIMER_LEFT_170=7,19 PRIMER_RIGHT_170=439,22 PRIMER_LEFT_170_TM=62.903 PRIMER_RIGHT_170_TM=57.992 PRIMER_LEFT_170_GC_PERCENT=63.158 PRIMER_RIGHT_170_GC_PERCENT=50.000 PRIMER_LEFT_170_SELF_ANY=5.00 PRIMER_RIGHT_170_SELF_ANY=4.00 PRIMER_LEFT_170_SELF_END=3.00 PRIMER_RIGHT_170_SELF_END=0.00 PRIMER_LEFT_170_END_STABILITY=6.7000 PRIMER_RIGHT_170_END_STABILITY=8.5000 PRIMER_PAIR_170_COMPL_ANY=4.00 PRIMER_PAIR_170_COMPL_END=0.00 PRIMER_PAIR_170_PRODUCT_SIZE=433 PRIMER_PAIR_171_PENALTY=7.950578 PRIMER_LEFT_171_PENALTY=7.562338 PRIMER_RIGHT_171_PENALTY=0.388240 PRIMER_LEFT_171_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTACC PRIMER_RIGHT_171_SEQUENCE=TCTGTGGCTTCTTCGTAGGG PRIMER_LEFT_171=407,25 PRIMER_RIGHT_171=999,20 PRIMER_LEFT_171_TM=62.562 PRIMER_RIGHT_171_TM=60.388 PRIMER_LEFT_171_GC_PERCENT=48.000 PRIMER_RIGHT_171_GC_PERCENT=55.000 PRIMER_LEFT_171_SELF_ANY=6.00 PRIMER_RIGHT_171_SELF_ANY=4.00 PRIMER_LEFT_171_SELF_END=1.00 PRIMER_RIGHT_171_SELF_END=0.00 PRIMER_LEFT_171_END_STABILITY=6.9000 PRIMER_RIGHT_171_END_STABILITY=8.7000 PRIMER_PAIR_171_COMPL_ANY=6.00 PRIMER_PAIR_171_COMPL_END=2.00 PRIMER_PAIR_171_PRODUCT_SIZE=593 PRIMER_PAIR_172_PENALTY=7.971303 PRIMER_LEFT_172_PENALTY=7.562338 PRIMER_RIGHT_172_PENALTY=0.408965 PRIMER_LEFT_172_SEQUENCE=CCTGCAGTATTGCCAAGAAGTCTAC PRIMER_RIGHT_172_SEQUENCE=TCATCGTCCTCGTCATCATC PRIMER_LEFT_172=406,25 PRIMER_RIGHT_172=945,20 PRIMER_LEFT_172_TM=62.562 PRIMER_RIGHT_172_TM=59.591 PRIMER_LEFT_172_GC_PERCENT=48.000 PRIMER_RIGHT_172_GC_PERCENT=50.000 PRIMER_LEFT_172_SELF_ANY=6.00 PRIMER_RIGHT_172_SELF_ANY=3.00 PRIMER_LEFT_172_SELF_END=3.00 PRIMER_RIGHT_172_SELF_END=1.00 PRIMER_LEFT_172_END_STABILITY=5.4000 PRIMER_RIGHT_172_END_STABILITY=6.6000 PRIMER_PAIR_172_COMPL_ANY=3.00 PRIMER_PAIR_172_COMPL_END=1.00 PRIMER_PAIR_172_PRODUCT_SIZE=540 PRIMER_PAIR_173_PENALTY=7.975020 PRIMER_LEFT_173_PENALTY=2.395059 PRIMER_RIGHT_173_PENALTY=5.579961 PRIMER_LEFT_173_SEQUENCE=TCAGGGACCAAAACCTGC PRIMER_RIGHT_173_SEQUENCE=CTGCTGTTGTAGGAAGTTTAACAGG PRIMER_LEFT_173=368,18 PRIMER_RIGHT_173=1304,25 PRIMER_LEFT_173_TM=59.605 PRIMER_RIGHT_173_TM=60.580 PRIMER_LEFT_173_GC_PERCENT=55.556 PRIMER_RIGHT_173_GC_PERCENT=44.000 PRIMER_LEFT_173_SELF_ANY=4.00 PRIMER_RIGHT_173_SELF_ANY=5.00 PRIMER_LEFT_173_SELF_END=2.00 PRIMER_RIGHT_173_SELF_END=1.00 PRIMER_LEFT_173_END_STABILITY=9.7000 PRIMER_RIGHT_173_END_STABILITY=7.9000 PRIMER_PAIR_173_COMPL_ANY=4.00 PRIMER_PAIR_173_COMPL_END=3.00 PRIMER_PAIR_173_PRODUCT_SIZE=937 PRIMER_PAIR_174_PENALTY=8.015547 PRIMER_LEFT_174_PENALTY=3.939502 PRIMER_RIGHT_174_PENALTY=4.076046 PRIMER_LEFT_174_SEQUENCE=TGACTCGCCTGGCTCTGA PRIMER_RIGHT_174_SEQUENCE=CAGGGTAGACTTCTTGGCAATACT PRIMER_LEFT_174=8,18 PRIMER_RIGHT_174=434,24 PRIMER_LEFT_174_TM=61.940 PRIMER_RIGHT_174_TM=60.076 PRIMER_LEFT_174_GC_PERCENT=61.111 PRIMER_RIGHT_174_GC_PERCENT=45.833 PRIMER_LEFT_174_SELF_ANY=5.00 PRIMER_RIGHT_174_SELF_ANY=5.00 PRIMER_LEFT_174_SELF_END=3.00 PRIMER_RIGHT_174_SELF_END=2.00 PRIMER_LEFT_174_END_STABILITY=6.7000 PRIMER_RIGHT_174_END_STABILITY=5.3000 PRIMER_PAIR_174_COMPL_ANY=4.00 PRIMER_PAIR_174_COMPL_END=1.00 PRIMER_PAIR_174_PRODUCT_SIZE=427 PRIMER_PAIR_175_PENALTY=8.072770 PRIMER_LEFT_175_PENALTY=2.420393 PRIMER_RIGHT_175_PENALTY=5.652377 PRIMER_LEFT_175_SEQUENCE=TGCCATGTTCTGTGGCAG PRIMER_RIGHT_175_SEQUENCE=ACTCTTCTCACTGCATGTCTCTT PRIMER_LEFT_175=301,18 PRIMER_RIGHT_175=685,23 PRIMER_LEFT_175_TM=60.420 PRIMER_RIGHT_175_TM=57.348 PRIMER_LEFT_175_GC_PERCENT=55.556 PRIMER_RIGHT_175_GC_PERCENT=43.478 PRIMER_LEFT_175_SELF_ANY=5.00 PRIMER_RIGHT_175_SELF_ANY=4.00 PRIMER_LEFT_175_SELF_END=3.00 PRIMER_RIGHT_175_SELF_END=0.00 PRIMER_LEFT_175_END_STABILITY=9.7000 PRIMER_RIGHT_175_END_STABILITY=6.7000 PRIMER_PAIR_175_COMPL_ANY=4.00 PRIMER_PAIR_175_COMPL_END=1.00 PRIMER_PAIR_175_PRODUCT_SIZE=385 PRIMER_PAIR_176_PENALTY=8.127519 PRIMER_LEFT_176_PENALTY=2.423358 PRIMER_RIGHT_176_PENALTY=5.704162 PRIMER_LEFT_176_SEQUENCE=TCCAGAATGGGAAGTGGGA PRIMER_RIGHT_176_SEQUENCE=CTTACAAACTCACCAACTAAGCAGC PRIMER_LEFT_176=339,19 PRIMER_RIGHT_176=570,25 PRIMER_LEFT_176_TM=61.423 PRIMER_RIGHT_176_TM=60.704 PRIMER_LEFT_176_GC_PERCENT=52.632 PRIMER_RIGHT_176_GC_PERCENT=44.000 PRIMER_LEFT_176_SELF_ANY=3.00 PRIMER_RIGHT_176_SELF_ANY=4.00 PRIMER_LEFT_176_SELF_END=0.00 PRIMER_RIGHT_176_SELF_END=3.00 PRIMER_LEFT_176_END_STABILITY=9.7000 PRIMER_RIGHT_176_END_STABILITY=9.7000 PRIMER_PAIR_176_COMPL_ANY=4.00 PRIMER_PAIR_176_COMPL_END=0.00 PRIMER_PAIR_176_PRODUCT_SIZE=232 PRIMER_PAIR_177_PENALTY=8.138201 PRIMER_LEFT_177_PENALTY=7.723082 PRIMER_RIGHT_177_PENALTY=0.415119 PRIMER_LEFT_177_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAACT PRIMER_RIGHT_177_SEQUENCE=TGTCAAAGTTGTTCCGGTTG PRIMER_LEFT_177=414,25 PRIMER_RIGHT_177=1196,20 PRIMER_LEFT_177_TM=62.723 PRIMER_RIGHT_177_TM=59.585 PRIMER_LEFT_177_GC_PERCENT=44.000 PRIMER_RIGHT_177_GC_PERCENT=45.000 PRIMER_LEFT_177_SELF_ANY=5.00 PRIMER_RIGHT_177_SELF_ANY=4.00 PRIMER_LEFT_177_SELF_END=1.00 PRIMER_RIGHT_177_SELF_END=0.00 PRIMER_LEFT_177_END_STABILITY=6.4000 PRIMER_RIGHT_177_END_STABILITY=8.2000 PRIMER_PAIR_177_COMPL_ANY=5.00 PRIMER_PAIR_177_COMPL_END=3.00 PRIMER_PAIR_177_PRODUCT_SIZE=783 PRIMER_PAIR_178_PENALTY=8.265617 PRIMER_LEFT_178_PENALTY=2.561455 PRIMER_RIGHT_178_PENALTY=5.704162 PRIMER_LEFT_178_SEQUENCE=GCACATGAATGTCCAGAATG PRIMER_RIGHT_178_SEQUENCE=GCTGCTGTTGTAGGAAGTTTAACAG PRIMER_LEFT_178=328,20 PRIMER_RIGHT_178=1305,25 PRIMER_LEFT_178_TM=57.439 PRIMER_RIGHT_178_TM=60.704 PRIMER_LEFT_178_GC_PERCENT=45.000 PRIMER_RIGHT_178_GC_PERCENT=44.000 PRIMER_LEFT_178_SELF_ANY=6.00 PRIMER_RIGHT_178_SELF_ANY=5.00 PRIMER_LEFT_178_SELF_END=1.00 PRIMER_RIGHT_178_SELF_END=3.00 PRIMER_LEFT_178_END_STABILITY=6.9000 PRIMER_RIGHT_178_END_STABILITY=6.7000 PRIMER_PAIR_178_COMPL_ANY=3.00 PRIMER_PAIR_178_COMPL_END=1.00 PRIMER_PAIR_178_PRODUCT_SIZE=978 PRIMER_PAIR_179_PENALTY=8.331209 PRIMER_LEFT_179_PENALTY=2.561455 PRIMER_RIGHT_179_PENALTY=5.769754 PRIMER_LEFT_179_SEQUENCE=TGCACATGAATGTCCAGAAT PRIMER_RIGHT_179_SEQUENCE=GCTGTTGTAGGAAGTTTAACAGG PRIMER_LEFT_179=327,20 PRIMER_RIGHT_179=1302,23 PRIMER_LEFT_179_TM=57.439 PRIMER_RIGHT_179_TM=57.230 PRIMER_LEFT_179_GC_PERCENT=40.000 PRIMER_RIGHT_179_GC_PERCENT=43.478 PRIMER_LEFT_179_SELF_ANY=8.00 PRIMER_RIGHT_179_SELF_ANY=5.00 PRIMER_LEFT_179_SELF_END=2.00 PRIMER_RIGHT_179_SELF_END=1.00 PRIMER_LEFT_179_END_STABILITY=6.6000 PRIMER_RIGHT_179_END_STABILITY=7.9000 PRIMER_PAIR_179_COMPL_ANY=3.00 PRIMER_PAIR_179_COMPL_END=0.00 PRIMER_PAIR_179_PRODUCT_SIZE=976 PRIMER_PAIR_180_PENALTY=8.348945 PRIMER_LEFT_180_PENALTY=2.561455 PRIMER_RIGHT_180_PENALTY=5.787490 PRIMER_LEFT_180_SEQUENCE=ATGCACATGAATGTCCAGAA PRIMER_RIGHT_180_SEQUENCE=GCTGTTGTAGGAAGTTTAACAGGAT PRIMER_LEFT_180=326,20 PRIMER_RIGHT_180=1302,25 PRIMER_LEFT_180_TM=57.439 PRIMER_RIGHT_180_TM=59.213 PRIMER_LEFT_180_GC_PERCENT=40.000 PRIMER_RIGHT_180_GC_PERCENT=40.000 PRIMER_LEFT_180_SELF_ANY=8.00 PRIMER_RIGHT_180_SELF_ANY=5.00 PRIMER_LEFT_180_SELF_END=1.00 PRIMER_RIGHT_180_SELF_END=2.00 PRIMER_LEFT_180_END_STABILITY=7.0000 PRIMER_RIGHT_180_END_STABILITY=7.8000 PRIMER_PAIR_180_COMPL_ANY=3.00 PRIMER_PAIR_180_COMPL_END=2.00 PRIMER_PAIR_180_PRODUCT_SIZE=977 PRIMER_PAIR_181_PENALTY=8.352989 PRIMER_LEFT_181_PENALTY=2.485003 PRIMER_RIGHT_181_PENALTY=5.867986 PRIMER_LEFT_181_SEQUENCE=TACCCACTGATGGTAATGCTG PRIMER_RIGHT_181_SEQUENCE=ACAAACTCACCAACTAAGCAGCG PRIMER_LEFT_181=258,21 PRIMER_RIGHT_181=567,23 PRIMER_LEFT_181_TM=58.515 PRIMER_RIGHT_181_TM=62.868 PRIMER_LEFT_181_GC_PERCENT=47.619 PRIMER_RIGHT_181_GC_PERCENT=47.826 PRIMER_LEFT_181_SELF_ANY=5.00 PRIMER_RIGHT_181_SELF_ANY=3.00 PRIMER_LEFT_181_SELF_END=1.00 PRIMER_RIGHT_181_SELF_END=2.00 PRIMER_LEFT_181_END_STABILITY=8.5000 PRIMER_RIGHT_181_END_STABILITY=10.2000 PRIMER_PAIR_181_COMPL_ANY=4.00 PRIMER_PAIR_181_COMPL_END=3.00 PRIMER_PAIR_181_PRODUCT_SIZE=310 PRIMER_PAIR_182_PENALTY=8.417976 PRIMER_LEFT_182_PENALTY=4.091249 PRIMER_RIGHT_182_PENALTY=4.326727 PRIMER_LEFT_182_SEQUENCE=GATCCCACTCGCACAGCA PRIMER_RIGHT_182_SEQUENCE=TTCAGGGTAGACTTCTTGGCAAT PRIMER_LEFT_182=153,18 PRIMER_RIGHT_182=436,23 PRIMER_LEFT_182_TM=62.091 PRIMER_RIGHT_182_TM=61.327 PRIMER_LEFT_182_GC_PERCENT=61.111 PRIMER_RIGHT_182_GC_PERCENT=43.478 PRIMER_LEFT_182_SELF_ANY=4.00 PRIMER_RIGHT_182_SELF_ANY=5.00 PRIMER_LEFT_182_SELF_END=0.00 PRIMER_RIGHT_182_SELF_END=3.00 PRIMER_LEFT_182_END_STABILITY=8.5000 PRIMER_RIGHT_182_END_STABILITY=8.4000 PRIMER_PAIR_182_COMPL_ANY=3.00 PRIMER_PAIR_182_COMPL_END=1.00 PRIMER_PAIR_182_PRODUCT_SIZE=284 PRIMER_PAIR_183_PENALTY=8.462996 PRIMER_LEFT_183_PENALTY=2.601441 PRIMER_RIGHT_183_PENALTY=5.861555 PRIMER_LEFT_183_SEQUENCE=GACCAAAACCTGCATTGATACC PRIMER_RIGHT_183_SEQUENCE=TACTTTGTCTTCACTCCCATCTGC PRIMER_LEFT_183=373,22 PRIMER_RIGHT_183=874,24 PRIMER_LEFT_183_TM=60.601 PRIMER_RIGHT_183_TM=61.862 PRIMER_LEFT_183_GC_PERCENT=45.455 PRIMER_RIGHT_183_GC_PERCENT=45.833 PRIMER_LEFT_183_SELF_ANY=4.00 PRIMER_RIGHT_183_SELF_ANY=2.00 PRIMER_LEFT_183_SELF_END=2.00 PRIMER_RIGHT_183_SELF_END=2.00 PRIMER_LEFT_183_END_STABILITY=6.8000 PRIMER_RIGHT_183_END_STABILITY=8.2000 PRIMER_PAIR_183_COMPL_ANY=4.00 PRIMER_PAIR_183_COMPL_END=0.00 PRIMER_PAIR_183_PRODUCT_SIZE=502 PRIMER_PAIR_184_PENALTY=8.522458 PRIMER_LEFT_184_PENALTY=2.578834 PRIMER_RIGHT_184_PENALTY=5.943624 PRIMER_LEFT_184_SEQUENCE=AATGTCCAGAATGGGAAGTG PRIMER_RIGHT_184_SEQUENCE=CTGGCTGCTGTTGTAGGAAGTTTA PRIMER_LEFT_184=335,20 PRIMER_RIGHT_184=1308,24 PRIMER_LEFT_184_TM=57.421 PRIMER_RIGHT_184_TM=61.944 PRIMER_LEFT_184_GC_PERCENT=45.000 PRIMER_RIGHT_184_GC_PERCENT=45.833 PRIMER_LEFT_184_SELF_ANY=3.00 PRIMER_RIGHT_184_SELF_ANY=3.00 PRIMER_LEFT_184_SELF_END=0.00 PRIMER_RIGHT_184_SELF_END=2.00 PRIMER_LEFT_184_END_STABILITY=6.7000 PRIMER_RIGHT_184_END_STABILITY=6.0000 PRIMER_PAIR_184_COMPL_ANY=4.00 PRIMER_PAIR_184_COMPL_END=0.00 PRIMER_PAIR_184_PRODUCT_SIZE=974 PRIMER_PAIR_185_PENALTY=8.617094 PRIMER_LEFT_185_PENALTY=2.601441 PRIMER_RIGHT_185_PENALTY=6.015654 PRIMER_LEFT_185_SEQUENCE=GGACCAAAACCTGCATTGATAC PRIMER_RIGHT_185_SEQUENCE=TCACTTACAAACTCACCAACTAAGC PRIMER_LEFT_185=372,22 PRIMER_RIGHT_185=573,25 PRIMER_LEFT_185_TM=60.601 PRIMER_RIGHT_185_TM=58.984 PRIMER_LEFT_185_GC_PERCENT=45.455 PRIMER_RIGHT_185_GC_PERCENT=40.000 PRIMER_LEFT_185_SELF_ANY=4.00 PRIMER_RIGHT_185_SELF_ANY=4.00 PRIMER_LEFT_185_SELF_END=2.00 PRIMER_RIGHT_185_SELF_END=2.00 PRIMER_LEFT_185_END_STABILITY=5.3000 PRIMER_RIGHT_185_END_STABILITY=7.5000 PRIMER_PAIR_185_COMPL_ANY=3.00 PRIMER_PAIR_185_COMPL_END=0.00 PRIMER_PAIR_185_PRODUCT_SIZE=202 PRIMER_PAIR_186_PENALTY=8.720488 PRIMER_LEFT_186_PENALTY=2.612614 PRIMER_RIGHT_186_PENALTY=6.107874 PRIMER_LEFT_186_SEQUENCE=CCATCAGGGACCAAAACCTG PRIMER_RIGHT_186_SEQUENCE=TACTACTTTGTCTTCACTCCCATCTG PRIMER_LEFT_186=365,20 PRIMER_RIGHT_186=877,26 PRIMER_LEFT_186_TM=62.613 PRIMER_RIGHT_186_TM=60.108 PRIMER_LEFT_186_GC_PERCENT=55.000 PRIMER_RIGHT_186_GC_PERCENT=42.308 PRIMER_LEFT_186_SELF_ANY=4.00 PRIMER_RIGHT_186_SELF_ANY=3.00 PRIMER_LEFT_186_SELF_END=1.00 PRIMER_RIGHT_186_SELF_END=2.00 PRIMER_LEFT_186_END_STABILITY=7.9000 PRIMER_RIGHT_186_END_STABILITY=6.6000 PRIMER_PAIR_186_COMPL_ANY=4.00 PRIMER_PAIR_186_COMPL_END=1.00 PRIMER_PAIR_186_PRODUCT_SIZE=513 PRIMER_PAIR_187_PENALTY=8.809127 PRIMER_LEFT_187_PENALTY=2.606855 PRIMER_RIGHT_187_PENALTY=6.202271 PRIMER_LEFT_187_SEQUENCE=CCAGAATGGGAAGTGGGA PRIMER_RIGHT_187_SEQUENCE=TACTCTTCTCACTGCATGTCTCTTT PRIMER_LEFT_187=340,18 PRIMER_RIGHT_187=686,25 PRIMER_LEFT_187_TM=59.393 PRIMER_RIGHT_187_TM=58.798 PRIMER_LEFT_187_GC_PERCENT=55.556 PRIMER_RIGHT_187_GC_PERCENT=40.000 PRIMER_LEFT_187_SELF_ANY=3.00 PRIMER_RIGHT_187_SELF_ANY=4.00 PRIMER_LEFT_187_SELF_END=0.00 PRIMER_RIGHT_187_SELF_END=0.00 PRIMER_LEFT_187_END_STABILITY=9.7000 PRIMER_RIGHT_187_END_STABILITY=7.0000 PRIMER_PAIR_187_COMPL_ANY=5.00 PRIMER_PAIR_187_COMPL_END=2.00 PRIMER_PAIR_187_PRODUCT_SIZE=347 PRIMER_PAIR_188_PENALTY=8.875619 PRIMER_LEFT_188_PENALTY=4.361392 PRIMER_RIGHT_188_PENALTY=4.514227 PRIMER_LEFT_188_SEQUENCE=TCCGTCAGTTTCCTCGGC PRIMER_RIGHT_188_SEQUENCE=AGTTCAGGGTAGACTTCTTGGCA PRIMER_LEFT_188=47,18 PRIMER_RIGHT_188=438,23 PRIMER_LEFT_188_TM=62.361 PRIMER_RIGHT_188_TM=61.514 PRIMER_LEFT_188_GC_PERCENT=61.111 PRIMER_RIGHT_188_GC_PERCENT=47.826 PRIMER_LEFT_188_SELF_ANY=3.00 PRIMER_RIGHT_188_SELF_ANY=4.00 PRIMER_LEFT_188_SELF_END=2.00 PRIMER_RIGHT_188_SELF_END=3.00 PRIMER_LEFT_188_END_STABILITY=11.4000 PRIMER_RIGHT_188_END_STABILITY=10.0000 PRIMER_PAIR_188_COMPL_ANY=3.00 PRIMER_PAIR_188_COMPL_END=1.00 PRIMER_PAIR_188_PRODUCT_SIZE=392 PRIMER_PAIR_189_PENALTY=8.875619 PRIMER_LEFT_189_PENALTY=4.361392 PRIMER_RIGHT_189_PENALTY=4.514227 PRIMER_LEFT_189_SEQUENCE=TCGGGCTCCGTCAGTTTC PRIMER_RIGHT_189_SEQUENCE=CAGTTCAGGGTAGACTTCTTGGC PRIMER_LEFT_189=41,18 PRIMER_RIGHT_189=439,23 PRIMER_LEFT_189_TM=62.361 PRIMER_RIGHT_189_TM=61.514 PRIMER_LEFT_189_GC_PERCENT=61.111 PRIMER_RIGHT_189_GC_PERCENT=52.174 PRIMER_LEFT_189_SELF_ANY=4.00 PRIMER_RIGHT_189_SELF_ANY=4.00 PRIMER_LEFT_189_SELF_END=0.00 PRIMER_RIGHT_189_SELF_END=2.00 PRIMER_LEFT_189_END_STABILITY=6.7000 PRIMER_RIGHT_189_END_STABILITY=10.0000 PRIMER_PAIR_189_COMPL_ANY=3.00 PRIMER_PAIR_189_COMPL_END=0.00 PRIMER_PAIR_189_PRODUCT_SIZE=399 PRIMER_PAIR_190_PENALTY=8.888679 PRIMER_LEFT_190_PENALTY=2.613581 PRIMER_RIGHT_190_PENALTY=6.275098 PRIMER_LEFT_190_SEQUENCE=CTGGCTGAACCCCAGATTG PRIMER_RIGHT_190_SEQUENCE=TGTCTTCACTCCCATCTGCATAGT PRIMER_LEFT_190=284,19 PRIMER_RIGHT_190=869,24 PRIMER_LEFT_190_TM=61.614 PRIMER_RIGHT_190_TM=62.275 PRIMER_LEFT_190_GC_PERCENT=57.895 PRIMER_RIGHT_190_GC_PERCENT=45.833 PRIMER_LEFT_190_SELF_ANY=4.00 PRIMER_RIGHT_190_SELF_ANY=4.00 PRIMER_LEFT_190_SELF_END=2.00 PRIMER_RIGHT_190_SELF_END=2.00 PRIMER_LEFT_190_END_STABILITY=6.9000 PRIMER_RIGHT_190_END_STABILITY=5.3000 PRIMER_PAIR_190_COMPL_ANY=5.00 PRIMER_PAIR_190_COMPL_END=1.00 PRIMER_PAIR_190_PRODUCT_SIZE=586 PRIMER_PAIR_191_PENALTY=8.911303 PRIMER_LEFT_191_PENALTY=4.397076 PRIMER_RIGHT_191_PENALTY=4.514227 PRIMER_LEFT_191_SEQUENCE=CGGTTTGGCACTGCTCCT PRIMER_RIGHT_191_SEQUENCE=GCAGTTCAGGGTAGACTTCTTGG PRIMER_LEFT_191=208,18 PRIMER_RIGHT_191=440,23 PRIMER_LEFT_191_TM=62.397 PRIMER_RIGHT_191_TM=61.514 PRIMER_LEFT_191_GC_PERCENT=61.111 PRIMER_RIGHT_191_GC_PERCENT=52.174 PRIMER_LEFT_191_SELF_ANY=5.00 PRIMER_RIGHT_191_SELF_ANY=4.00 PRIMER_LEFT_191_SELF_END=0.00 PRIMER_RIGHT_191_SELF_END=0.00 PRIMER_LEFT_191_END_STABILITY=7.9000 PRIMER_RIGHT_191_END_STABILITY=8.5000 PRIMER_PAIR_191_COMPL_ANY=5.00 PRIMER_PAIR_191_COMPL_END=1.00 PRIMER_PAIR_191_PRODUCT_SIZE=233 PRIMER_PAIR_192_PENALTY=8.943335 PRIMER_LEFT_192_PENALTY=2.606855 PRIMER_RIGHT_192_PENALTY=6.336480 PRIMER_LEFT_192_SEQUENCE=TCCAGAATGGGAAGTGGG PRIMER_RIGHT_192_SEQUENCE=ACTCTTCTCACTGCATGTCTCTTTG PRIMER_LEFT_192=339,18 PRIMER_RIGHT_192=685,25 PRIMER_LEFT_192_TM=59.393 PRIMER_RIGHT_192_TM=61.336 PRIMER_LEFT_192_GC_PERCENT=55.556 PRIMER_RIGHT_192_GC_PERCENT=44.000 PRIMER_LEFT_192_SELF_ANY=3.00 PRIMER_RIGHT_192_SELF_ANY=4.00 PRIMER_LEFT_192_SELF_END=0.00 PRIMER_RIGHT_192_SELF_END=0.00 PRIMER_LEFT_192_END_STABILITY=9.4000 PRIMER_RIGHT_192_END_STABILITY=7.3000 PRIMER_PAIR_192_COMPL_ANY=5.00 PRIMER_PAIR_192_COMPL_END=1.00 PRIMER_PAIR_192_PRODUCT_SIZE=347 PRIMER_PAIR_193_PENALTY=8.974531 PRIMER_LEFT_193_PENALTY=8.555845 PRIMER_RIGHT_193_PENALTY=0.418685 PRIMER_LEFT_193_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_193_SEQUENCE=CGGCCATGCAGTACTCTTCT PRIMER_LEFT_193=412,26 PRIMER_RIGHT_193=1217,20 PRIMER_LEFT_193_TM=62.556 PRIMER_RIGHT_193_TM=60.419 PRIMER_LEFT_193_GC_PERCENT=46.154 PRIMER_RIGHT_193_GC_PERCENT=55.000 PRIMER_LEFT_193_SELF_ANY=5.00 PRIMER_RIGHT_193_SELF_ANY=6.00 PRIMER_LEFT_193_SELF_END=0.00 PRIMER_RIGHT_193_SELF_END=1.00 PRIMER_LEFT_193_END_STABILITY=6.7000 PRIMER_RIGHT_193_END_STABILITY=6.7000 PRIMER_PAIR_193_COMPL_ANY=6.00 PRIMER_PAIR_193_COMPL_END=3.00 PRIMER_PAIR_193_PRODUCT_SIZE=806 PRIMER_PAIR_194_PENALTY=8.981355 PRIMER_LEFT_194_PENALTY=2.631568 PRIMER_RIGHT_194_PENALTY=6.349786 PRIMER_LEFT_194_SEQUENCE=ATCCTGCAGTATTGCCAAGAAG PRIMER_RIGHT_194_SEQUENCE=TGCTGTTGTAGGAAGTTTAACAGGA PRIMER_LEFT_194=404,22 PRIMER_RIGHT_194=1303,25 PRIMER_LEFT_194_TM=60.632 PRIMER_RIGHT_194_TM=61.350 PRIMER_LEFT_194_GC_PERCENT=45.455 PRIMER_RIGHT_194_GC_PERCENT=40.000 PRIMER_LEFT_194_SELF_ANY=8.00 PRIMER_RIGHT_194_SELF_ANY=5.00 PRIMER_LEFT_194_SELF_END=2.00 PRIMER_RIGHT_194_SELF_END=3.00 PRIMER_LEFT_194_END_STABILITY=6.7000 PRIMER_RIGHT_194_END_STABILITY=8.2000 PRIMER_PAIR_194_COMPL_ANY=5.00 PRIMER_PAIR_194_COMPL_END=3.00 PRIMER_PAIR_194_PRODUCT_SIZE=900 PRIMER_PAIR_195_PENALTY=9.037893 PRIMER_LEFT_195_PENALTY=2.617395 PRIMER_RIGHT_195_PENALTY=6.420498 PRIMER_LEFT_195_SEQUENCE=GTACCCACTGATGGTAATGCTG PRIMER_RIGHT_195_SEQUENCE=TTCTACTACTTTGTCTTCACTCCCA PRIMER_LEFT_195=257,22 PRIMER_RIGHT_195=880,25 PRIMER_LEFT_195_TM=59.383 PRIMER_RIGHT_195_TM=58.580 PRIMER_LEFT_195_GC_PERCENT=50.000 PRIMER_RIGHT_195_GC_PERCENT=40.000 PRIMER_LEFT_195_SELF_ANY=5.00 PRIMER_RIGHT_195_SELF_ANY=3.00 PRIMER_LEFT_195_SELF_END=1.00 PRIMER_RIGHT_195_SELF_END=0.00 PRIMER_LEFT_195_END_STABILITY=8.5000 PRIMER_RIGHT_195_END_STABILITY=9.7000 PRIMER_PAIR_195_COMPL_ANY=4.00 PRIMER_PAIR_195_COMPL_END=3.00 PRIMER_PAIR_195_PRODUCT_SIZE=624 PRIMER_PAIR_196_PENALTY=9.052346 PRIMER_LEFT_196_PENALTY=2.618102 PRIMER_RIGHT_196_PENALTY=6.434244 PRIMER_LEFT_196_SEQUENCE=CTGAACCCCAGATTGCCA PRIMER_RIGHT_196_SEQUENCE=GTACTCTTCTCACTGCATGTCTCTTT PRIMER_LEFT_196=288,18 PRIMER_RIGHT_196=687,26 PRIMER_LEFT_196_TM=60.618 PRIMER_RIGHT_196_TM=59.566 PRIMER_LEFT_196_GC_PERCENT=55.556 PRIMER_RIGHT_196_GC_PERCENT=42.308 PRIMER_LEFT_196_SELF_ANY=3.00 PRIMER_RIGHT_196_SELF_ANY=4.00 PRIMER_LEFT_196_SELF_END=3.00 PRIMER_RIGHT_196_SELF_END=0.00 PRIMER_LEFT_196_END_STABILITY=10.0000 PRIMER_RIGHT_196_END_STABILITY=7.0000 PRIMER_PAIR_196_COMPL_ANY=3.00 PRIMER_PAIR_196_COMPL_END=1.00 PRIMER_PAIR_196_PRODUCT_SIZE=400 PRIMER_PAIR_197_PENALTY=9.095316 PRIMER_LEFT_197_PENALTY=2.644351 PRIMER_RIGHT_197_PENALTY=6.450965 PRIMER_LEFT_197_SEQUENCE=ATTCAGATCCATCAGGGACCA PRIMER_RIGHT_197_SEQUENCE=ACTACTTTGTCTTCACTCCCATCT PRIMER_LEFT_197=357,21 PRIMER_RIGHT_197=876,24 PRIMER_LEFT_197_TM=61.644 PRIMER_RIGHT_197_TM=57.549 PRIMER_LEFT_197_GC_PERCENT=47.619 PRIMER_RIGHT_197_GC_PERCENT=41.667 PRIMER_LEFT_197_SELF_ANY=5.00 PRIMER_RIGHT_197_SELF_ANY=2.00 PRIMER_LEFT_197_SELF_END=3.00 PRIMER_RIGHT_197_SELF_END=0.00 PRIMER_LEFT_197_END_STABILITY=7.9000 PRIMER_RIGHT_197_END_STABILITY=6.6000 PRIMER_PAIR_197_COMPL_ANY=4.00 PRIMER_PAIR_197_COMPL_END=1.00 PRIMER_PAIR_197_PRODUCT_SIZE=520 PRIMER_PAIR_198_PENALTY=9.096848 PRIMER_LEFT_198_PENALTY=8.647019 PRIMER_RIGHT_198_PENALTY=0.449829 PRIMER_LEFT_198_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAACT PRIMER_RIGHT_198_SEQUENCE=TCGGCTTCTTCTTCTTCCAC PRIMER_LEFT_198=413,26 PRIMER_RIGHT_198=927,20 PRIMER_LEFT_198_TM=62.647 PRIMER_RIGHT_198_TM=59.550 PRIMER_LEFT_198_GC_PERCENT=42.308 PRIMER_RIGHT_198_GC_PERCENT=50.000 PRIMER_LEFT_198_SELF_ANY=5.00 PRIMER_RIGHT_198_SELF_ANY=2.00 PRIMER_LEFT_198_SELF_END=1.00 PRIMER_RIGHT_198_SELF_END=0.00 PRIMER_LEFT_198_END_STABILITY=6.4000 PRIMER_RIGHT_198_END_STABILITY=7.9000 PRIMER_PAIR_198_COMPL_ANY=6.00 PRIMER_PAIR_198_COMPL_END=2.00 PRIMER_PAIR_198_PRODUCT_SIZE=515 PRIMER_PAIR_199_PENALTY=9.102169 PRIMER_LEFT_199_PENALTY=8.647019 PRIMER_RIGHT_199_PENALTY=0.455150 PRIMER_LEFT_199_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_199_SEQUENCE=CCTCTCTTTGGCTTTCTGGA PRIMER_LEFT_199=411,26 PRIMER_RIGHT_199=1390,20 PRIMER_LEFT_199_TM=62.647 PRIMER_RIGHT_199_TM=59.545 PRIMER_LEFT_199_GC_PERCENT=42.308 PRIMER_RIGHT_199_GC_PERCENT=50.000 PRIMER_LEFT_199_SELF_ANY=5.00 PRIMER_RIGHT_199_SELF_ANY=2.00 PRIMER_LEFT_199_SELF_END=0.00 PRIMER_RIGHT_199_SELF_END=2.00 PRIMER_LEFT_199_END_STABILITY=7.0000 PRIMER_RIGHT_199_END_STABILITY=8.2000 PRIMER_PAIR_199_COMPL_ANY=5.00 PRIMER_PAIR_199_COMPL_END=3.00 PRIMER_PAIR_199_PRODUCT_SIZE=980 = SEQUENCE_ID=ok_regions_test2 SEQUENCE_TEMPLATE=GCGGAAGGAGGGAGGGGGCCGCGCTCGGCGCCCCGGCCGGGCCACTGGGCCACAGGCCACGCGGCCACGCAGTCCGAGCGGGAGCCGAGCCGGGCGGGGCGAGGGCAGCTCCGGGAAGGAACGTCCCAGGGATGGAAGTGCTTGGATGCGGTGCTGCTGGCTGCGGATGTGCGCAAGGAGATGGGATGGAGAGCCTGAGTTGGCATTCGTATAAATGACCTGCCTGGCTCCCACCATGAGTGCTGAGCTTAACGTGCCTATCGACCCCTCTGCTCCTGCCTGCCCTGAGCCCGGCCATAAGGGCATGGATTACCGGGACTGGGTCCGCCGCAGCTACCTGGAACTGGTCACCTCTAACCACCACTCGGTACAGGCCCTGTCGTGGCGGAAGCTCTACCTGAGCAGGGCCAAGCTGAAGGCCTCCAGCAGGACCTCCGCCCTCCTCTCCGGCTTTGCCATGGTGGCCATGGTGGAGGTGCAGCTGGAGACGCAGTACCAGTACCCGCGGCCGCTGCTGATTGCCTTCAGCGCCTGCACCACGGTGCTGGTGGCCGTGCACCTGTTCGCCCTCCTCATCAGCACCTGCATCCTGCCCAATGTGGAGGCCGTGAGCAACATCCACAACCTGAACTCCATCAGCGAGTCCCCGCATGAGCGCATGCACCCCTACATCGAGCTGGCCTGGGGCTTCTCCACCGTGCTTGGCATCCTACTCTTCCTGGCCGAGGTGGTGCTGCTCTGCTGGATCAAGTTCCTCCCCGTGGATGCCCGGCGCCAGCCTGGCCCCCCACCTGGCCCTGGGAGTCACACGGGCTGGCAGGCCGCCCTGGTGTCCACCATCATCATGGTGCCCGTGGGCCTCATCTTCGTGGTCTTCACCATCCACTTCTACCGCTCCCTGGTGCGCCACAAAACGGAGCGCCACAACCGCGAGATCGAGGAGCTCCACAAGCTCAAGGTCCAGCTGGACGGGCATGAGCGCAGCCTGCAGGTCTTGTGAGGGGCCGAGGGCCGGGGCTGGGAGCGGCCCTGTGCCCGGGAGTCCGCAGAGGCGGGGATTTGTCAGATGCAGACATTTTGCAAGGCTGCCGGGTAGTTCAAGACCAAAGTTTTCCTCTTGTCTTAATACCATAAGGACTGGATGACTTCTCCTGAGATAGAACCGTTTGGTTCAATGAGGGACTGTGTTGCTAAGAGCGTTGGGGGCAAAGCCAGGCTGGTTCCTTGGCCTCGGGGTTTCCTGGGTCGGGGACACGGTGAAGAGGCTCCAGCGGGACCTGCCCATCAGTCCTGGGCCAGGAGGGGCTCCAAGCAGCACCCAGCGGTCCGGGGGAGTCTCAGACCCGGCATGCGTGGCTGGCAGACCTGGGAGAGCCAGGGCAGGGTTTTGCGTTCAGAGAAGGATTGCCCCAGAGACCCGTGGTGGACTTCATGGGTGCTGAGTGGCCCGTGTGACAGTGATGACACGAAGGCTTCGGCGTTTGAGTGGGTGCAGGTGCACGCCAGGGCTTGGTGCTTCCCTGCCTGGCCCTGGAGGGAGCTGGGTGGCCTGGCTTCAGGGGAAGACAGGAGCCAGGACACACGTCAGCCCAGCAGGTGTGGGGGGTGCTGCAGCCCTCGGCAGTGGGGTCAGGCCCTGGGGGATGTTTCCAATGGTGGGCAGCCTGGCCAGGCCGGAGAAGACATGTTCACGGGCATCTATCAGATGCCCCCTTGAGGAGGCTGAGTTATTTGAGGGCTGCTGCAAAGTACGCTAGGCTCAAATTCTCTTTTCCCAGCCAGAGCCCTGGCCACACGGACTCAGAGGGGCCACCGGGGTGGGGAAAGGACCCCTCCCCCACCCCCCGCAGCCACTGGCCTCCAGCTCTCGGCCACAGAATGGCCTCTAAGGCTGACTCAGCCACTCCCTTGGGCTGTGGCAGCAGGAGGCGGGGGCTCTGGCTCAGGCCCCGGAGCCTGTGCAGCTTGCCCATGGCCCTAGGCAGCGAGGGGACAGCCTGGGGGACTTCCTGCCTAGGCAAGGTCATTGGCCGGGCCTGGCCTGTGGATAGTGGGGCCAGGGGCCGGCCCAGGCCAAATGAGTGCCCTCCTTGTTATGACACCAAGTGACTACAAGGGAGGCAAGACCCCTCCAGGCCTCTCAGCCGACACTGGGTCCCACCACACACAGTGACTGTGCCGTGCAGTGCAGGTTCTGGCCTTTTCCTTGAAGGCATCTGGTAGACCCGAAGCCACGCTCTCGGGCCGCACATGCACGCCGCAGCACCAGCTGCCCTGAGCTGCTTGTACAACCAAACACCTTTCCCCTCTTCTCCAGCTGTAACCTGGAGAGTCAGCCATGCCTTGTCTTTTGTTCTCATAAATAGTCACTGGGGCCGGGCGCAGTGACTCACGCCTGTAATCCCAGCACTTTGGGAGGCCTAGGTGGGCGGATCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAAAATACAGAAAATTAGCTGGGCGTGGTGGCGGGCGCCTGTAGCCCCAGCTACTTGGGAGGCTGAGGCGGGAGAATGGCAATGGCGTGAACCCGGGAGGCAGAGCTTGCAGTGAGCTGAGATGGCGCCACTGCACTCCAGCCTGGGCGACAGAGCCAGACTCCATCTCAAAAAAAAAAAAAAAATTAGCCGGGCGTGATGGCGGGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAAGAGAATCACTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCACGCCATTGCACTACAGCCTGGGCAACAAGAGCGAAACTTTGTCTAAAAAAAAAAAAAAATCACTGGGCTTCTTCATGCTCTGATCACATCTCTCGTAAAAGCTTAAGCTCTCTCCGGGGTCCGGGTTGGCCGTGCCGTGGAATTCTGGGTGGCCTGGCTGGGGTCTCTGGAAATGTGGCTGCAGCAGAGAACAGAGACCCTGACATGCAGTTTTCCGTGCTGAGGGGCCCTAGGGGAGTCACACCAAGGGTCCCCACGAGAAAGTTGTGGCATCCCCGGGGGCCGGAGAAGAGCCCCGTGTCTTCTGAGGGGTTCGTCCTTTGTGTCCCCTGCAGACATTTGTCTGCGACCTTTGCCCTCCAGCATGTATGTACTTTCCTGCAGCCTGTAGAAACGCCTCTTACGGTTTAATATGTGTTCGCTTTGCTAAAGAATATCAACATCGGCCAGGCGAGGTGGGGCACGCCTGTCATCCCAGCACTTTGGGAGGCTGAGGTGGGAGGATCACTTGGGCCCAGGGGTGCAAGACCAGCCTGGGCAACATAGCGAGACCCCATGTCTAAAAAAATTATTTTAAATTAGCCAGGCCGGGTGCAATGGCTCGCGCCCGTAATCTTAGCACTCTGGGAGGCCGAGGCAGGCAGATCACTTGAGATCAGGACTTTAAGACCAGCCTCGGCAACAACATGGTGAAACCATCTCTAGCAAAAATACAAAAAATTAGCCGGGTATGGTGGCGGGTACCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAAGAGAATCGCTTGAACGCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCGTGCCACTGCACTCCAGCCTGGACAACAGAGCAAAACTCTGTCTCAAAAAATAATAAATAAAAATAAATTAGCTGGGCGTGGTGGTGCATGCCTGTAGTTCCAGCTACTTGGGAGGCTGAGGTGGGAGGATTGCTTGAGCCTGGGAAGTAGAGGCTGCAGTGAACTATAACTGTGCTAGTGGCCGGGCGCAGTGGCTCACGCCTATAATCCCAGCACTTTGGGAGGCCAAAGCAGGTGGATCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACTCTGTGTCTACTAAAAATACAAAAAATTAGCTGGGTGTGGTGGCGCTCGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGAACCCAGAAGGCAGAGGTAGCAGTGAGCCAAGATGGTGCCACTGCACTCCAGCCTGGGCGACAGAGGGTGACTCCTCAAAAAAAAGAAAAATAGCTAAAATATGATTGTACCACTGCACTCCAGCCTGGGTGACAGAGTGAGACCCTGTCTCTAAAAAAAAAAAAAAGAAAATAAAGAAAAAAGAATGTCAGCATGTCAGCGTTCCTCCAAGGAGTGGCTTTGGGTCAAGAAGACAGGCTGGCTCTCCTAAACAAACGGCTCCCCACGGCTGGGAGAGGCAGCAGGCCCTTACTCTGGCCTGAGTCTTTTGGGAGGGGCCCTGCTGGGCCTTCTCCTGCCACCTACCTTCCTGGACCATCTGTGCCCCTGCCCACGCAGTACCACACTCCCCAGACTGTGGAACGCATCTCCAGTGCCATCTGGGGGCCAGTTACTGTACCTGGTCCCCAGGCAGAGCTCTTGGCCTGGCAGCATCCAGGCAGGTATTAAGTGCCCAGTGATGGTGGCACATGGGTGGGGACGGAGCCACATCCAAGTCTAGCCCTCTGATTAGGAGGATGACAGTCTTGGAATTTCTTGTTCTCTTTCTCCTAGGGTTCTCGTTCTCCCAGCTCCAGCCTTTCTAGTTTCGAGGCATGAGGCAAACCATTGCTGTGGGAATAGCAGTGACCATTGGTAGGGCCATGGTGGCCGGGAGAGGAAACCTCAGGGTCACCCCAGAGCCTGTGGGGAGACGGAGGGTGAGCTGTTGTTGGAGCTGCAGAAACGCCTTGAAGGGCAGCATGGGCGATGAGGAATGCCATTGCAGCCTAGAAATTCGGTTTCTGGAGGTTTAGCCTGATTTTGAAGAGTATTTAAGAACAGAGGGCTCCAGACCAGGTGTGGTGGCTCACATCTGTAATCCCAGCACTTTGGGAGGCAGAGGTGGGCGGATCGCCTGAGATCAGGAGTTCGAGACCAGCCTGGCCCACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTAGTGACACACGCCTGTAATCCCAGCTGCTCGGGAGGCAGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCACGCCACTGCACTCCAGCCTGGGTAACAGAGCAAGACTCTGTCTCAAAAAAAAAAAAAAAAGAACAGGGGCCTCTGCCTCAGTTTGCCCGGGAGCCAGCCAGGGCCCATCCTAATTTGGAGCACAGTCTTCCTGGTGCCTAGACATGCCAAGGCCCCTCCCACGTGGTACACCCTCTCCGTTTAGTACCTGACCACCTGTTTCAAAACGCAGGTGTTTCTGGTTTAGAAACTTGGAAGGCGGAATGTGTTTTCGTGTCTTCTAGGAAGGGTCTGCTGAGGACCAGACCACGTAAGCCTGAGTGGATCCTGACTCAGCTGCAGCCCTTACCTGCCTCGTGCTGATGATCTATGCATGGCGTTATGTAGATCACGTGCGGCAGAGACAGCCACTGTCCTGTGTGCGGGTTTTTAAAACAGCTGCCCTGGATGAAACGGAATAAACCAGTGATGCTATTCAAAGTCTTTCTGTTCTTGGGGGATGCTGTGGGCAGAGGGGATAAATTGCAGCCCCTGGCCCTCCCGTGGCATCTGCTGACTGGGGGACCCACATCGTGGCCTGAAAAACTCCTGGAGCGCCCTCTGGCCTCCTGTCGCTGGTCACTGGCCAGTGTCCTCCCAGGCTGCCCCGGCAAGGGGGCGTGGACATCTCTAGAATGGAGGCAGGGTACTCATGTCCAGACACGCCGCCATGGGGCTCCGCCCCGAGAGCGCAGGGTGCCTCTGCCTGGCGCTTCCCCTCCCAGACAGGGCCCTCTCTTTCCAGAAACAAAAGAAGGGGCAGCCCCAGAGGGTGGCTGAGCGACAACAGAGCCCACCTGGACAGGGTCAGCTTGGCGTTCCCCCCAGTCCCTTGAGGAGAGGAGCTACCAGAGCACTGGTCACCTCAGGACATTCTCCAGCCCCAAGTCCCTGTGGGGCAGCAGGAACAGGTGGGTCCCTCCTGCGGGCTGGTGGCACTGCTAAGGAAGCACCAGACAGCCTTTTTTTTTTTTTTGAGACAGAGTCGCTCTGTCACCCAAGCTGGAATGCGGTGGCATGATCTTGGCTCACTGCAACCTCCTCCTCCCAGGTTCAAGCGATCTTGTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCACGCACCACCATGCTTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCATGACCTCGTGATCTGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCAGCTTGCCCAGCTTATGTTTATAGATTTATTTTAGAGACAGGGTTGGGCTCTATTGCCCAGGCCAGAGCTGGAGTGCAGTGGTGCAATCATAGCTCTCTGCAGGCTCCAGCTTCTGGGCGCAAGCAATCCTCCCACCTCAGTCTCCCAAGTAGCTACACTTGGGAGACTACGGGCGCACACTACCATGCCCAGCTAATGTTTTTTTTGTAGAGATGGGATCTTGCTATGTTGCCCAGTCTGGTCTTGAACTTCTGGCCTCAAGCGACCCTCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGCGAGCCACCATGCCTGGCCCATTCTGTTTTTTGTCGGCCACTGAGGTAGAGGCCCTCTTCTAGGAAAGAATTTCTGTTTGTTTTGTTTTGTTTTGTTTTGTTTTTTTGAAACAGTCTCTCTCTGTCTCCCAGGCTGGAGTGCAGTGGCGTGATCTCAGCTCGCTGCAACCTCCACTTCCCAGGTTCAAGCAATTCTTCTGCCAAGCTGGGACTACAGGCATGCACCACCACCATGCCCTGCTAATTTTTGTTGTTGTCGTTGGTTTTTGAGATAGAGTTTCGCTCTTGTTGCCCAGGCTGGAGTGCAGTGGCACGATCTCAGCTCACCGCAACCTCCGCCTCCCAGGTTCAAGCAATTATCTTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCATGCGCCACCACACCTGGCTTATTTTTGTATTTTTAGTAGAGATGGGGTTTCTCCATGTTAGCCAAGCTGGTCTCGAACTCCCGACCTCAGATGATCCGCCTGCCTCAGCCTCCCAAAGTGCTGGGGTTACAGGTGTGAGCCACCACACCCAGCCTTTCTTTTTGCATTTTTAATAGAGACTGAGTTTCATCACACTGGCCAGGCTGGTTTCGAACTCCTGGCCTCAAGTGATCCGCTGGCCTCAGCCTCCTAAAGTGCTGGATTACAGGCATGAGCCACCGTGCCAGGCCTTGTTAATATTTTTAACTCTGGCACTTCACCCTAGAGGCTGCTAGGAATCAGGTGTGCCAAGCAACTGGCACCACTCCATGGAAAGCGTCTAGGGCAGTGCCTTGGTTCTGCCAATCCTGAGAAGCCAGGCTGCCCCTTGGGCTGGGCAGTGGCCTCAGTACCGAGCCCCAGGCCAGCAAGCCCACAAGCCTCGAGAAAGACTGCACTTCCGGTGGAGGCTGCAGTTTCCTTAAAGGGAAAATCTGCCCCACCCCTGTCAAGGGCAGGACACAAGTCACTGGCATTGAAGGACCCACTCCACACCCAGCAGATCATCGACGCAGACTTTTTTTTTCCCAGATAGAGTCTCACTCTGTCGTCCAGGCTGGACTGCAATGGTGTGATCTTGGCTCACTGTAACCTCCGCCTCCCGGGCTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTAGGATTACAGGCGCCCACCATCACGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTTGCCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGATCCTCCTGCCTCAGCCTCCCAAAGTGCTGGGATTATAGGCGTGAGCCACCTCGTCCGGCCCAAGACTTTTTGCCTCAGATGCTGCAGAGCCCTGGTGGGGCCCAGGAGGGCAAGGCCCACACAGGAGCCGAAACCCATGTAGAACCTGCCAACCTGTAGCTCACACCTATAATCCCAGCACTTTGGGAGGCTGAGGTGGGCGGATCGCTTGAGCCAAGGAGTTCGAGACCAGCCTGGGCAACATTACGAGACCCTGTCTCTACAAAAATACAAAAATTACCCGGGCTTGGTGGCTCGTGCCTATAGTCCCAGCTACTGGGGAGGCTGAGGTGGGAGAATCATCTGAGCCCAGGGAGGTTGAGACTGCAGTGAGCCAAGATTGTGCCACTGCACTCCAGCCTGGGTAAAATGAGTGAGACCACGTCTCAACCCCTTTCACAGCCCGTCTGCGGCCCACCCACCCATGGGTACTGTGGGATGGGGGACAGGCTGGCTTAACACAAATCGAGGCAGGAATAACCCCAGAGAATGGGCTTTGCATGGAGCTTGGCTCCTGTCCCTGCCTGTGAGGGAGGACCAGACTCGGCCTCACCACCTGCCACTCTGAGCAAACAGGCAACGGTGTTTCCTGAACATCTTTCTGAAGCGGCTGAGGGATGTCAGCTGAGCCCCCGCTGGGCCTGCTCTGGAGCGGGATGTCTCCAGAAGCCGCCCTTGGAGCGGGCACTTCCCTATTTGGGCGTGTCCCAGTCCCATGCCTCACCATCCCCTTGCTTGAAGCTCCAAGAGCATGAGAGTGGGCAGCCTGGTCTGCTGAGGAAAGTGTCTGATGGATGCGGAAATGGCCACCCCAAACACCGGTAAGCAGATGTTACCCTGCAGGCGGTGGCTCCTGGGGCCCAGCCCTGCAGAAACACATGGGGCAGGCTGGGCAGAGGGGCTCACACCCGTTATTCCCAGCGCTTTGGGAGGCTGAGGCGGGAGGATCGCTTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACATAGCAAGACTCTATCTCCACTAAAAATCAAAACAAAACAATTAGCTGGGTATGGTGGCACACGCCTGTAGTTCCAGCTACTGGGGAGGCTGAGGGGGAGGATCACTTGAGCCCAGGAGTTCAAGGCTGCAGTGAGCCATGATTGCGCCACTGCACTCCAGCCTGGGCAACAGAGCAAGACCCTGCCCCGCCGCCTGAAAGAAAGAAAAAAGAAAGAGAAAGGAAGAGAAAGAAAGGAAAAGAAAAAGGAAACATACGAGGCTATGGGGCCGTGTACGGGGGCCAGGCTGTGGCAGAAGCATCCTGGCGTGGCCTTCTCCAGCCCCATCCTGGTTGGGTAAGGTGTCGCCGTGGGTCTTCGTGTCACAGCTTCAAGGACAGACCCAACTCTGGTATCACAAACTTAACAGGTAATGGAGTTTCCTAAAGTGTGCTCTGCAGGAGTGCTGGAAGGGCCAGCATGGAAGGACCCCAGGGAGGGAGATTTCTTTGCTCCACAGCATCCCCAAGCCTCACGAGGCCTCTCCCCTCCAAGTCTCTATGTGCATCGTTCTCCAGATGTTGGAGCATCCTGCAGGGGCCAGCCCAGCCTGTCAGGGGCAGAGACCCTGCACTGGCCCCCTCGAGAGTGGACCCCTGGGCTGGCTTATCTGCTCCAGTGCAGTGAGGATCAGCTGGAGCTCCGACCTGACTCACATCTGGATGCGCAGTGAGGGGCCCGTTGGGGCCGTGATACTGGGAGGAAGCCGGGCTCTGTGCTCTTGCTCCAAGTGAGTTGACCAGGGAGCCGAAGTTAAGGTGGCCTGGGAGCCACGTCCTCCCACCTAGCGGCTGGGCTCTCACAGCCACTAGAAACCCATCAGCCTTTCCCCTGGGTCTATGCGGGGCTCTTCAGCCCTCCTGGGAGCTCAGTGGCTGGGATGCTGCTTTACAGACAGTGCACGGCTCTGCCATCTGACAGCTCCCAGAGGGTTCTGGGGGTGACTGTAAAGAGTGGTGCTGGCCCCGAACCTTTCCTGTTCCCCTGGATTTAGTTCCCAGCAGGTGGTGAGGAACTGAACATTTTGTCCCATAGTGGTGGGAGAGAGGGCTGGCCACATGGGTCCAGGCCACCCCCTCAGCCTCCACAGCCTGCCCCGCACCCAGCCACAGGAACAGCAGAGCAGCTCCCCCATCCAGGTAAGCGCCCCCACCACAGCCCCATCACAAAGGAATAGCCTCCTGGTGTAGTGGGAGGTGAGGAGCTTTTTTTGTTTTCCTTTTATTTATTTTAGGGACAGGGTCTTGCTCTGTTGCCAAGTTAAGTGCAGTGGTATGATCATGAGCTCACTGCAGCCTTGACATGGTGGTAAAGCCATCCTCCCGCCTCAGCCTTTCGAGTAGCTGGGACTACAGGTGTGTGCCACCATGCCCAGACGCCCAGCTAATGTTTGTATTTTTTGTAGAGATGGGGGTCTCACTATGTTGCCCAGGCTGGCCTCAAGCAATCCTCCCACCTGAGCCCCGCAAAGTACTCGGATTACAGGTGTGAGTCACCGCGCCTGGCCGGGAGCTTTAATTTTTCCTACTTGTTAACAGCGTTATGGGGCTGGAAGGAGGCTCTCAGATTTCCCTGCCCTCCTCACATCCCCCAACCCTCCCTGGGCGTGGGGGTGTCAAGCATGAGTCAGGCTGAGCCTGACTCTGAAGCTGTTCCCAGCTCTTTAGTCTAAACTTCAAATCTTCAACCCTGTTCCCTAATCCAACACATTCCCCAAATGAAGGGACTGGACAGTGAGATATCGGGAAACCCCATGAAAATAGATGCATAATAAAGGTGAAAATCATT PRIMER_NUM_RETURN=100 PRIMER_PRODUCT_SIZE_RANGE=75-800 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,251,,;,,1,251; PRIMER_MIN_THREE_PRIME_DISTANCE=0 PRIMER_LEFT_EXPLAIN=considered 106940, GC content failed 617, low tm 78, high tm 1421, high end compl 39, not in any ok left region 104635, ok 150 PRIMER_RIGHT_EXPLAIN=considered 106876, GC content failed 647, low tm 334, high tm 7627, high any compl 3, high end compl 29, long poly-x seq 10, not in any ok right region 97330, ok 896 PRIMER_PAIR_EXPLAIN=considered 1174, unacceptable product size 987, high any compl 1, high end compl 56, primer in pair overlaps a primer in a better pair 39258, ok 130 PRIMER_LEFT_NUM_RETURNED=100 PRIMER_RIGHT_NUM_RETURNED=100 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=100 PRIMER_PAIR_0_PENALTY=0.085464 PRIMER_LEFT_0_PENALTY=0.042732 PRIMER_RIGHT_0_PENALTY=0.042732 PRIMER_LEFT_0_SEQUENCE=GAGAGCCTGAGTTGGCATTC PRIMER_RIGHT_0_SEQUENCE=GGAACTTGATCCAGCAGAGC PRIMER_LEFT_0=188,20 PRIMER_RIGHT_0=754,20 PRIMER_LEFT_0_TM=59.957 PRIMER_RIGHT_0_TM=59.957 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=567 PRIMER_PAIR_1_PENALTY=0.489932 PRIMER_LEFT_1_PENALTY=0.394220 PRIMER_RIGHT_1_PENALTY=0.095713 PRIMER_LEFT_1_SEQUENCE=CAAGGAGATGGGATGGAGAG PRIMER_RIGHT_1_SEQUENCE=AGGGAGCGGTAGAAGTGGAT PRIMER_LEFT_1=173,20 PRIMER_RIGHT_1=899,20 PRIMER_LEFT_1_TM=59.606 PRIMER_RIGHT_1_TM=60.096 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=2.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=727 PRIMER_PAIR_2_PENALTY=0.696500 PRIMER_LEFT_2_PENALTY=0.562760 PRIMER_RIGHT_2_PENALTY=0.133741 PRIMER_LEFT_2_SEQUENCE=AAGGAGATGGGATGGAGAGC PRIMER_RIGHT_2_SEQUENCE=AGCGGTAGAAGTGGATGGTG PRIMER_LEFT_2=174,20 PRIMER_RIGHT_2=895,20 PRIMER_LEFT_2_TM=60.563 PRIMER_RIGHT_2_TM=60.134 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=2.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=722 PRIMER_PAIR_3_PENALTY=0.963400 PRIMER_LEFT_3_PENALTY=0.796337 PRIMER_RIGHT_3_PENALTY=0.167063 PRIMER_LEFT_3_SEQUENCE=AGCCTGAGTTGGCATTCGTA PRIMER_RIGHT_3_SEQUENCE=CGGTAATCCATGCCCTTATG PRIMER_LEFT_3=191,20 PRIMER_RIGHT_3=314,20 PRIMER_LEFT_3_TM=60.796 PRIMER_RIGHT_3_TM=60.167 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.4000 PRIMER_RIGHT_3_END_STABILITY=6.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=124 PRIMER_PAIR_4_PENALTY=1.036721 PRIMER_LEFT_4_PENALTY=0.846380 PRIMER_RIGHT_4_PENALTY=0.190341 PRIMER_LEFT_4_SEQUENCE=ATAAATGACCTGCCTGGCTC PRIMER_RIGHT_4_SEQUENCE=GAAGACCACGAAGATGAGGC PRIMER_LEFT_4=210,20 PRIMER_RIGHT_4=876,20 PRIMER_LEFT_4_TM=59.154 PRIMER_RIGHT_4_TM=59.810 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=667 PRIMER_PAIR_5_PENALTY=1.216058 PRIMER_LEFT_5_PENALTY=1.022938 PRIMER_RIGHT_5_PENALTY=0.193120 PRIMER_LEFT_5_SEQUENCE=ATGGGATGGAGAGCCTGAGT PRIMER_RIGHT_5_SEQUENCE=CGGGTACTGGTACTGCGTCT PRIMER_LEFT_5=180,20 PRIMER_RIGHT_5=504,20 PRIMER_LEFT_5_TM=61.023 PRIMER_RIGHT_5_TM=60.193 PRIMER_LEFT_5_GC_PERCENT=55.000 PRIMER_RIGHT_5_GC_PERCENT=60.000 PRIMER_LEFT_5_SELF_ANY=3.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_END_STABILITY=6.4000 PRIMER_RIGHT_5_END_STABILITY=8.1000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PAIR_5_PRODUCT_SIZE=325 PRIMER_PAIR_6_PENALTY=1.242484 PRIMER_LEFT_6_PENALTY=1.032614 PRIMER_RIGHT_6_PENALTY=0.209870 PRIMER_LEFT_6_SEQUENCE=TCGTATAAATGACCTGCCTGG PRIMER_RIGHT_6_SEQUENCE=CCAGGAAGAGTAGGATGCCA PRIMER_LEFT_6=206,21 PRIMER_RIGHT_6=721,20 PRIMER_LEFT_6_TM=59.967 PRIMER_RIGHT_6_TM=60.210 PRIMER_LEFT_6_GC_PERCENT=47.619 PRIMER_RIGHT_6_GC_PERCENT=55.000 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=3.00 PRIMER_LEFT_6_END_STABILITY=9.7000 PRIMER_RIGHT_6_END_STABILITY=10.0000 PRIMER_PAIR_6_COMPL_ANY=6.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=516 PRIMER_PAIR_7_PENALTY=1.364928 PRIMER_LEFT_7_PENALTY=1.168025 PRIMER_RIGHT_7_PENALTY=0.196903 PRIMER_LEFT_7_SEQUENCE=ATGGGATGGAGAGCCTGAG PRIMER_RIGHT_7_SEQUENCE=GGAGGAACTTGATCCAGCAG PRIMER_LEFT_7=180,19 PRIMER_RIGHT_7=757,20 PRIMER_LEFT_7_TM=60.168 PRIMER_RIGHT_7_TM=59.803 PRIMER_LEFT_7_GC_PERCENT=57.895 PRIMER_RIGHT_7_GC_PERCENT=55.000 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=3.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_END_STABILITY=6.7000 PRIMER_RIGHT_7_END_STABILITY=8.2000 PRIMER_PAIR_7_COMPL_ANY=5.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PAIR_7_PRODUCT_SIZE=578 PRIMER_PAIR_8_PENALTY=1.392367 PRIMER_LEFT_8_PENALTY=1.168025 PRIMER_RIGHT_8_PENALTY=0.224341 PRIMER_LEFT_8_SEQUENCE=AGATGGGATGGAGAGCCTG PRIMER_RIGHT_8_SEQUENCE=TAGGCACGTTAAGCTCAGCA PRIMER_LEFT_8=178,19 PRIMER_RIGHT_8=259,20 PRIMER_LEFT_8_TM=60.168 PRIMER_RIGHT_8_TM=59.776 PRIMER_LEFT_8_GC_PERCENT=57.895 PRIMER_RIGHT_8_GC_PERCENT=50.000 PRIMER_LEFT_8_SELF_ANY=3.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_LEFT_8_SELF_END=1.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_END_STABILITY=9.7000 PRIMER_RIGHT_8_END_STABILITY=8.5000 PRIMER_PAIR_8_COMPL_ANY=4.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PAIR_8_PRODUCT_SIZE=82 PRIMER_PAIR_9_PENALTY=1.476386 PRIMER_LEFT_9_PENALTY=1.185797 PRIMER_RIGHT_9_PENALTY=0.290589 PRIMER_LEFT_9_SEQUENCE=GCCTGAGTTGGCATTCGTA PRIMER_RIGHT_9_SEQUENCE=CTGCTCAGGTAGAGCTTCCG PRIMER_LEFT_9=192,19 PRIMER_RIGHT_9=404,20 PRIMER_LEFT_9_TM=59.814 PRIMER_RIGHT_9_TM=60.291 PRIMER_LEFT_9_GC_PERCENT=52.632 PRIMER_RIGHT_9_GC_PERCENT=60.000 PRIMER_LEFT_9_SELF_ANY=3.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_END_STABILITY=7.4000 PRIMER_RIGHT_9_END_STABILITY=10.2000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=2.00 PRIMER_PAIR_9_PRODUCT_SIZE=213 PRIMER_PAIR_10_PENALTY=1.629195 PRIMER_LEFT_10_PENALTY=1.365052 PRIMER_RIGHT_10_PENALTY=0.264143 PRIMER_LEFT_10_SEQUENCE=GGAGAGCCTGAGTTGGCAT PRIMER_RIGHT_10_SEQUENCE=GGTCGATAGGCACGTTAAGC PRIMER_LEFT_10=187,19 PRIMER_RIGHT_10=265,20 PRIMER_LEFT_10_TM=60.365 PRIMER_RIGHT_10_TM=59.736 PRIMER_LEFT_10_GC_PERCENT=57.895 PRIMER_RIGHT_10_GC_PERCENT=55.000 PRIMER_LEFT_10_SELF_ANY=3.00 PRIMER_RIGHT_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=2.00 PRIMER_LEFT_10_END_STABILITY=9.6000 PRIMER_RIGHT_10_END_STABILITY=7.5000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PAIR_10_PRODUCT_SIZE=79 PRIMER_PAIR_11_PENALTY=1.649114 PRIMER_LEFT_11_PENALTY=1.606382 PRIMER_RIGHT_11_PENALTY=0.042732 PRIMER_LEFT_11_SEQUENCE=AGGGATGGAAGTGCTTGGA PRIMER_RIGHT_11_SEQUENCE=GAATGCCAACTCAGGCTCTC PRIMER_LEFT_11=127,19 PRIMER_RIGHT_11=207,20 PRIMER_LEFT_11_TM=60.606 PRIMER_RIGHT_11_TM=59.957 PRIMER_LEFT_11_GC_PERCENT=52.632 PRIMER_RIGHT_11_GC_PERCENT=55.000 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=3.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=1.00 PRIMER_LEFT_11_END_STABILITY=8.5000 PRIMER_RIGHT_11_END_STABILITY=6.4000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PAIR_11_PRODUCT_SIZE=81 PRIMER_PAIR_12_PENALTY=1.682987 PRIMER_LEFT_12_PENALTY=1.365052 PRIMER_RIGHT_12_PENALTY=0.317935 PRIMER_LEFT_12_SEQUENCE=ATGGAGAGCCTGAGTTGGC PRIMER_RIGHT_12_SEQUENCE=TCAGGTTGTGGATGTTGCTC PRIMER_LEFT_12=185,19 PRIMER_RIGHT_12=628,20 PRIMER_LEFT_12_TM=60.365 PRIMER_RIGHT_12_TM=59.682 PRIMER_LEFT_12_GC_PERCENT=57.895 PRIMER_RIGHT_12_GC_PERCENT=50.000 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=2.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_END_STABILITY=10.0000 PRIMER_RIGHT_12_END_STABILITY=8.2000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PAIR_12_PRODUCT_SIZE=444 PRIMER_PAIR_13_PENALTY=1.747525 PRIMER_LEFT_13_PENALTY=1.423250 PRIMER_RIGHT_13_PENALTY=0.324275 PRIMER_LEFT_13_SEQUENCE=CCACCATGAGTGCTGAGCTT PRIMER_RIGHT_13_SEQUENCE=TGGTGAAGACCACGAAGATG PRIMER_LEFT_13=230,20 PRIMER_RIGHT_13=880,20 PRIMER_LEFT_13_TM=61.423 PRIMER_RIGHT_13_TM=59.676 PRIMER_LEFT_13_GC_PERCENT=55.000 PRIMER_RIGHT_13_GC_PERCENT=50.000 PRIMER_LEFT_13_SELF_ANY=6.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=0.00 PRIMER_LEFT_13_END_STABILITY=8.2000 PRIMER_RIGHT_13_END_STABILITY=6.6000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PAIR_13_PRODUCT_SIZE=651 PRIMER_PAIR_14_PENALTY=1.917502 PRIMER_LEFT_14_PENALTY=1.814316 PRIMER_RIGHT_14_PENALTY=0.103187 PRIMER_LEFT_14_SEQUENCE=GATGGAAGTGCTTGGATGC PRIMER_RIGHT_14_SEQUENCE=ATACGAATGCCAACTCAGGC PRIMER_LEFT_14=130,19 PRIMER_RIGHT_14=211,20 PRIMER_LEFT_14_TM=59.186 PRIMER_RIGHT_14_TM=60.103 PRIMER_LEFT_14_GC_PERCENT=52.632 PRIMER_RIGHT_14_GC_PERCENT=50.000 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=4.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_END_STABILITY=8.1000 PRIMER_RIGHT_14_END_STABILITY=9.7000 PRIMER_PAIR_14_COMPL_ANY=4.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PAIR_14_PRODUCT_SIZE=82 PRIMER_PAIR_15_PENALTY=1.979863 PRIMER_LEFT_15_PENALTY=1.571682 PRIMER_RIGHT_15_PENALTY=0.408180 PRIMER_LEFT_15_SEQUENCE=GCAAGGAGATGGGATGGAG PRIMER_RIGHT_15_SEQUENCE=GGGTCGATAGGCACGTTAAG PRIMER_LEFT_15=172,19 PRIMER_RIGHT_15=266,20 PRIMER_LEFT_15_TM=60.572 PRIMER_RIGHT_15_TM=59.592 PRIMER_LEFT_15_GC_PERCENT=57.895 PRIMER_RIGHT_15_GC_PERCENT=55.000 PRIMER_LEFT_15_SELF_ANY=2.00 PRIMER_RIGHT_15_SELF_ANY=4.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_END_STABILITY=8.2000 PRIMER_RIGHT_15_END_STABILITY=6.3000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=95 PRIMER_PAIR_16_PENALTY=2.110563 PRIMER_LEFT_16_PENALTY=1.702162 PRIMER_RIGHT_16_PENALTY=0.408401 PRIMER_LEFT_16_SEQUENCE=GGAGAGCCTGAGTTGGCATT PRIMER_RIGHT_16_SEQUENCE=CTCGCTGATGGAGTTCAGGT PRIMER_LEFT_16=187,20 PRIMER_RIGHT_16=642,20 PRIMER_LEFT_16_TM=61.702 PRIMER_RIGHT_16_TM=60.408 PRIMER_LEFT_16_GC_PERCENT=55.000 PRIMER_RIGHT_16_GC_PERCENT=55.000 PRIMER_LEFT_16_SELF_ANY=3.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=1.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_END_STABILITY=8.4000 PRIMER_RIGHT_16_END_STABILITY=7.9000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PAIR_16_PRODUCT_SIZE=456 PRIMER_PAIR_17_PENALTY=2.186862 PRIMER_LEFT_17_PENALTY=1.778461 PRIMER_RIGHT_17_PENALTY=0.408401 PRIMER_LEFT_17_SEQUENCE=TGGGATGGAGAGCCTGAGT PRIMER_RIGHT_17_SEQUENCE=ACTCGCTGATGGAGTTCAGG PRIMER_LEFT_17=181,19 PRIMER_RIGHT_17=643,20 PRIMER_LEFT_17_TM=60.778 PRIMER_RIGHT_17_TM=60.408 PRIMER_LEFT_17_GC_PERCENT=57.895 PRIMER_RIGHT_17_GC_PERCENT=55.000 PRIMER_LEFT_17_SELF_ANY=3.00 PRIMER_RIGHT_17_SELF_ANY=5.00 PRIMER_LEFT_17_SELF_END=1.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_END_STABILITY=6.4000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=3.00 PRIMER_PAIR_17_PRODUCT_SIZE=463 PRIMER_PAIR_18_PENALTY=2.223373 PRIMER_LEFT_18_PENALTY=1.804687 PRIMER_RIGHT_18_PENALTY=0.418685 PRIMER_LEFT_18_SEQUENCE=GGGATGGAGAGCCTGAGTT PRIMER_RIGHT_18_SEQUENCE=AGAGTAGGATGCCAAGCACG PRIMER_LEFT_18=182,19 PRIMER_RIGHT_18=715,20 PRIMER_LEFT_18_TM=59.195 PRIMER_RIGHT_18_TM=60.419 PRIMER_LEFT_18_GC_PERCENT=57.895 PRIMER_RIGHT_18_GC_PERCENT=55.000 PRIMER_LEFT_18_SELF_ANY=3.00 PRIMER_RIGHT_18_SELF_ANY=3.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_END_STABILITY=6.4000 PRIMER_RIGHT_18_END_STABILITY=9.9000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PAIR_18_PRODUCT_SIZE=534 PRIMER_PAIR_19_PENALTY=2.237985 PRIMER_LEFT_19_PENALTY=1.817214 PRIMER_RIGHT_19_PENALTY=0.420772 PRIMER_LEFT_19_SEQUENCE=AGCCTGAGTTGGCATTCGT PRIMER_RIGHT_19_SEQUENCE=GGTGACCAGTTCCAGGTAGC PRIMER_LEFT_19=191,19 PRIMER_RIGHT_19=351,20 PRIMER_LEFT_19_TM=60.817 PRIMER_RIGHT_19_TM=59.579 PRIMER_LEFT_19_GC_PERCENT=52.632 PRIMER_RIGHT_19_GC_PERCENT=60.000 PRIMER_LEFT_19_SELF_ANY=3.00 PRIMER_RIGHT_19_SELF_ANY=5.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_END_STABILITY=8.4000 PRIMER_RIGHT_19_END_STABILITY=6.9000 PRIMER_PAIR_19_COMPL_ANY=4.00 PRIMER_PAIR_19_COMPL_END=2.00 PRIMER_PAIR_19_PRODUCT_SIZE=161 PRIMER_PAIR_20_PENALTY=2.249787 PRIMER_LEFT_20_PENALTY=1.804465 PRIMER_RIGHT_20_PENALTY=0.445321 PRIMER_LEFT_20_SEQUENCE=TATAAATGACCTGCCTGGCTC PRIMER_RIGHT_20_SEQUENCE=CTTGTGGAGCTCCTCGATCT PRIMER_LEFT_20=209,21 PRIMER_RIGHT_20=951,20 PRIMER_LEFT_20_TM=59.196 PRIMER_RIGHT_20_TM=59.555 PRIMER_LEFT_20_GC_PERCENT=47.619 PRIMER_RIGHT_20_GC_PERCENT=55.000 PRIMER_LEFT_20_SELF_ANY=5.00 PRIMER_RIGHT_20_SELF_ANY=8.00 PRIMER_LEFT_20_SELF_END=3.00 PRIMER_RIGHT_20_SELF_END=3.00 PRIMER_LEFT_20_END_STABILITY=9.4000 PRIMER_RIGHT_20_END_STABILITY=6.3000 PRIMER_PAIR_20_COMPL_ANY=4.00 PRIMER_PAIR_20_COMPL_END=1.00 PRIMER_PAIR_20_PRODUCT_SIZE=743 PRIMER_PAIR_21_PENALTY=2.333688 PRIMER_LEFT_21_PENALTY=1.853206 PRIMER_RIGHT_21_PENALTY=0.480482 PRIMER_LEFT_21_SEQUENCE=TCCCACCATGAGTGCTGAG PRIMER_RIGHT_21_SEQUENCE=TGGAGTTCAGGTTGTGGATG PRIMER_LEFT_21=228,19 PRIMER_RIGHT_21=634,20 PRIMER_LEFT_21_TM=60.853 PRIMER_RIGHT_21_TM=59.520 PRIMER_LEFT_21_GC_PERCENT=57.895 PRIMER_RIGHT_21_GC_PERCENT=50.000 PRIMER_LEFT_21_SELF_ANY=6.00 PRIMER_RIGHT_21_SELF_ANY=3.00 PRIMER_LEFT_21_SELF_END=3.00 PRIMER_RIGHT_21_SELF_END=0.00 PRIMER_LEFT_21_END_STABILITY=6.7000 PRIMER_RIGHT_21_END_STABILITY=8.1000 PRIMER_PAIR_21_COMPL_ANY=5.00 PRIMER_PAIR_21_COMPL_END=0.00 PRIMER_PAIR_21_PRODUCT_SIZE=407 PRIMER_PAIR_22_PENALTY=2.333702 PRIMER_LEFT_22_PENALTY=1.853220 PRIMER_RIGHT_22_PENALTY=0.480482 PRIMER_LEFT_22_SEQUENCE=GAGATGGGATGGAGAGCCT PRIMER_RIGHT_22_SEQUENCE=TGATGGAGTTCAGGTTGTGG PRIMER_LEFT_22=177,19 PRIMER_RIGHT_22=637,20 PRIMER_LEFT_22_TM=59.147 PRIMER_RIGHT_22_TM=59.520 PRIMER_LEFT_22_GC_PERCENT=57.895 PRIMER_RIGHT_22_GC_PERCENT=50.000 PRIMER_LEFT_22_SELF_ANY=3.00 PRIMER_RIGHT_22_SELF_ANY=3.00 PRIMER_LEFT_22_SELF_END=3.00 PRIMER_RIGHT_22_SELF_END=0.00 PRIMER_LEFT_22_END_STABILITY=9.4000 PRIMER_RIGHT_22_END_STABILITY=8.2000 PRIMER_PAIR_22_COMPL_ANY=3.00 PRIMER_PAIR_22_COMPL_END=1.00 PRIMER_PAIR_22_PRODUCT_SIZE=461 PRIMER_PAIR_23_PENALTY=2.355333 PRIMER_LEFT_23_PENALTY=1.879714 PRIMER_RIGHT_23_PENALTY=0.475619 PRIMER_LEFT_23_SEQUENCE=CGTATAAATGACCTGCCTGG PRIMER_RIGHT_23_SEQUENCE=ACTGGTACTGCGTCTCCAGC PRIMER_LEFT_23=207,20 PRIMER_RIGHT_23=499,20 PRIMER_LEFT_23_TM=58.120 PRIMER_RIGHT_23_TM=60.476 PRIMER_LEFT_23_GC_PERCENT=50.000 PRIMER_RIGHT_23_GC_PERCENT=60.000 PRIMER_LEFT_23_SELF_ANY=4.00 PRIMER_RIGHT_23_SELF_ANY=4.00 PRIMER_LEFT_23_SELF_END=3.00 PRIMER_RIGHT_23_SELF_END=2.00 PRIMER_LEFT_23_END_STABILITY=9.7000 PRIMER_RIGHT_23_END_STABILITY=9.7000 PRIMER_PAIR_23_COMPL_ANY=4.00 PRIMER_PAIR_23_COMPL_END=3.00 PRIMER_PAIR_23_PRODUCT_SIZE=293 PRIMER_PAIR_24_PENALTY=2.363551 PRIMER_LEFT_24_PENALTY=1.853220 PRIMER_RIGHT_24_PENALTY=0.510331 PRIMER_LEFT_24_SEQUENCE=AGGAGATGGGATGGAGAGC PRIMER_RIGHT_24_SEQUENCE=GGATGGTGAAGACCACGAAG PRIMER_LEFT_24=175,19 PRIMER_RIGHT_24=883,20 PRIMER_LEFT_24_TM=59.147 PRIMER_RIGHT_24_TM=60.510 PRIMER_LEFT_24_GC_PERCENT=57.895 PRIMER_RIGHT_24_GC_PERCENT=55.000 PRIMER_LEFT_24_SELF_ANY=2.00 PRIMER_RIGHT_24_SELF_ANY=4.00 PRIMER_LEFT_24_SELF_END=2.00 PRIMER_RIGHT_24_SELF_END=0.00 PRIMER_LEFT_24_END_STABILITY=7.9000 PRIMER_RIGHT_24_END_STABILITY=8.7000 PRIMER_PAIR_24_COMPL_ANY=3.00 PRIMER_PAIR_24_COMPL_END=1.00 PRIMER_PAIR_24_PRODUCT_SIZE=709 PRIMER_PAIR_25_PENALTY=2.533215 PRIMER_LEFT_25_PENALTY=1.975728 PRIMER_RIGHT_25_PENALTY=0.557487 PRIMER_LEFT_25_SEQUENCE=GGATGGAAGTGCTTGGATG PRIMER_RIGHT_25_SEQUENCE=CCGAGTGGTGGTTAGAGGTG PRIMER_LEFT_25=129,19 PRIMER_RIGHT_25=367,20 PRIMER_LEFT_25_TM=59.024 PRIMER_RIGHT_25_TM=60.557 PRIMER_LEFT_25_GC_PERCENT=52.632 PRIMER_RIGHT_25_GC_PERCENT=60.000 PRIMER_LEFT_25_SELF_ANY=4.00 PRIMER_RIGHT_25_SELF_ANY=2.00 PRIMER_LEFT_25_SELF_END=2.00 PRIMER_RIGHT_25_SELF_END=0.00 PRIMER_LEFT_25_END_STABILITY=8.1000 PRIMER_RIGHT_25_END_STABILITY=7.9000 PRIMER_PAIR_25_COMPL_ANY=3.00 PRIMER_PAIR_25_COMPL_END=0.00 PRIMER_PAIR_25_PRODUCT_SIZE=239 PRIMER_PAIR_26_PENALTY=2.595918 PRIMER_LEFT_26_PENALTY=2.006951 PRIMER_RIGHT_26_PENALTY=0.588967 PRIMER_LEFT_26_SEQUENCE=GTATAAATGACCTGCCTGGCTC PRIMER_RIGHT_26_SEQUENCE=AGCTGGACCTTGAGCTTGTG PRIMER_LEFT_26=208,22 PRIMER_RIGHT_26=965,20 PRIMER_LEFT_26_TM=59.993 PRIMER_RIGHT_26_TM=60.589 PRIMER_LEFT_26_GC_PERCENT=50.000 PRIMER_RIGHT_26_GC_PERCENT=55.000 PRIMER_LEFT_26_SELF_ANY=5.00 PRIMER_RIGHT_26_SELF_ANY=4.00 PRIMER_LEFT_26_SELF_END=3.00 PRIMER_RIGHT_26_SELF_END=0.00 PRIMER_LEFT_26_END_STABILITY=9.4000 PRIMER_RIGHT_26_END_STABILITY=7.0000 PRIMER_PAIR_26_COMPL_ANY=4.00 PRIMER_PAIR_26_COMPL_END=2.00 PRIMER_PAIR_26_PRODUCT_SIZE=758 PRIMER_PAIR_27_PENALTY=2.646531 PRIMER_LEFT_27_PENALTY=2.057564 PRIMER_RIGHT_27_PENALTY=0.588967 PRIMER_LEFT_27_SEQUENCE=AGCCTGAGTTGGCATTCG PRIMER_RIGHT_27_SEQUENCE=CAGCTGGACCTTGAGCTTGT PRIMER_LEFT_27=191,18 PRIMER_RIGHT_27=966,20 PRIMER_LEFT_27_TM=59.942 PRIMER_RIGHT_27_TM=60.589 PRIMER_LEFT_27_GC_PERCENT=55.556 PRIMER_RIGHT_27_GC_PERCENT=55.000 PRIMER_LEFT_27_SELF_ANY=3.00 PRIMER_RIGHT_27_SELF_ANY=6.00 PRIMER_LEFT_27_SELF_END=2.00 PRIMER_RIGHT_27_SELF_END=0.00 PRIMER_LEFT_27_END_STABILITY=8.6000 PRIMER_RIGHT_27_END_STABILITY=6.7000 PRIMER_PAIR_27_COMPL_ANY=3.00 PRIMER_PAIR_27_COMPL_END=0.00 PRIMER_PAIR_27_PRODUCT_SIZE=776 PRIMER_PAIR_28_PENALTY=2.707656 PRIMER_LEFT_28_PENALTY=2.106022 PRIMER_RIGHT_28_PENALTY=0.601633 PRIMER_LEFT_28_SEQUENCE=AGATGGGATGGAGAGCCTGA PRIMER_RIGHT_28_SEQUENCE=AGGCACGTTAAGCTCAGCAC PRIMER_LEFT_28=178,20 PRIMER_RIGHT_28=258,20 PRIMER_LEFT_28_TM=62.106 PRIMER_RIGHT_28_TM=60.602 PRIMER_LEFT_28_GC_PERCENT=55.000 PRIMER_RIGHT_28_GC_PERCENT=55.000 PRIMER_LEFT_28_SELF_ANY=3.00 PRIMER_RIGHT_28_SELF_ANY=5.00 PRIMER_LEFT_28_SELF_END=1.00 PRIMER_RIGHT_28_SELF_END=1.00 PRIMER_LEFT_28_END_STABILITY=8.2000 PRIMER_RIGHT_28_END_STABILITY=7.9000 PRIMER_PAIR_28_COMPL_ANY=4.00 PRIMER_PAIR_28_COMPL_END=3.00 PRIMER_PAIR_28_PRODUCT_SIZE=81 PRIMER_PAIR_29_PENALTY=2.707656 PRIMER_LEFT_29_PENALTY=2.106022 PRIMER_RIGHT_29_PENALTY=0.601633 PRIMER_LEFT_29_SEQUENCE=GAGATGGGATGGAGAGCCTG PRIMER_RIGHT_29_SEQUENCE=GGCACGTTAAGCTCAGCACT PRIMER_LEFT_29=177,20 PRIMER_RIGHT_29=257,20 PRIMER_LEFT_29_TM=62.106 PRIMER_RIGHT_29_TM=60.602 PRIMER_LEFT_29_GC_PERCENT=60.000 PRIMER_RIGHT_29_GC_PERCENT=55.000 PRIMER_LEFT_29_SELF_ANY=3.00 PRIMER_RIGHT_29_SELF_ANY=5.00 PRIMER_LEFT_29_SELF_END=1.00 PRIMER_RIGHT_29_SELF_END=2.00 PRIMER_LEFT_29_END_STABILITY=9.7000 PRIMER_RIGHT_29_END_STABILITY=7.9000 PRIMER_PAIR_29_COMPL_ANY=4.00 PRIMER_PAIR_29_COMPL_END=3.00 PRIMER_PAIR_29_PRODUCT_SIZE=81 PRIMER_PAIR_30_PENALTY=2.781272 PRIMER_LEFT_30_PENALTY=2.071143 PRIMER_RIGHT_30_PENALTY=0.710129 PRIMER_LEFT_30_SEQUENCE=GGAGAGCCTGAGTTGGCA PRIMER_RIGHT_30_SEQUENCE=ACTTGATCCAGCAGAGCAGC PRIMER_LEFT_30=187,18 PRIMER_RIGHT_30=751,20 PRIMER_LEFT_30_TM=60.071 PRIMER_RIGHT_30_TM=60.710 PRIMER_LEFT_30_GC_PERCENT=61.111 PRIMER_RIGHT_30_GC_PERCENT=55.000 PRIMER_LEFT_30_SELF_ANY=3.00 PRIMER_RIGHT_30_SELF_ANY=6.00 PRIMER_LEFT_30_SELF_END=3.00 PRIMER_RIGHT_30_SELF_END=3.00 PRIMER_LEFT_30_END_STABILITY=10.0000 PRIMER_RIGHT_30_END_STABILITY=9.7000 PRIMER_PAIR_30_COMPL_ANY=3.00 PRIMER_PAIR_30_COMPL_END=1.00 PRIMER_PAIR_30_PRODUCT_SIZE=565 PRIMER_PAIR_31_PENALTY=2.793543 PRIMER_LEFT_31_PENALTY=2.071143 PRIMER_RIGHT_31_PENALTY=0.722400 PRIMER_LEFT_31_SEQUENCE=TGGAGAGCCTGAGTTGGC PRIMER_RIGHT_31_SEQUENCE=CAGGTTGTGGATGTTGCTCA PRIMER_LEFT_31=186,18 PRIMER_RIGHT_31=627,20 PRIMER_LEFT_31_TM=60.071 PRIMER_RIGHT_31_TM=60.722 PRIMER_LEFT_31_GC_PERCENT=61.111 PRIMER_RIGHT_31_GC_PERCENT=50.000 PRIMER_LEFT_31_SELF_ANY=3.00 PRIMER_RIGHT_31_SELF_ANY=2.00 PRIMER_LEFT_31_SELF_END=2.00 PRIMER_RIGHT_31_SELF_END=2.00 PRIMER_LEFT_31_END_STABILITY=10.0000 PRIMER_RIGHT_31_END_STABILITY=8.2000 PRIMER_PAIR_31_COMPL_ANY=4.00 PRIMER_PAIR_31_COMPL_END=2.00 PRIMER_PAIR_31_PRODUCT_SIZE=442 PRIMER_PAIR_32_PENALTY=2.851602 PRIMER_LEFT_32_PENALTY=2.106022 PRIMER_RIGHT_32_PENALTY=0.745579 PRIMER_LEFT_32_SEQUENCE=GATGGGATGGAGAGCCTGAG PRIMER_RIGHT_32_SEQUENCE=AGGAGCAGAGGGGTCGATAG PRIMER_LEFT_32=179,20 PRIMER_RIGHT_32=276,20 PRIMER_LEFT_32_TM=62.106 PRIMER_RIGHT_32_TM=60.746 PRIMER_LEFT_32_GC_PERCENT=60.000 PRIMER_RIGHT_32_GC_PERCENT=60.000 PRIMER_LEFT_32_SELF_ANY=3.00 PRIMER_RIGHT_32_SELF_ANY=4.00 PRIMER_LEFT_32_SELF_END=3.00 PRIMER_RIGHT_32_SELF_END=2.00 PRIMER_LEFT_32_END_STABILITY=6.7000 PRIMER_RIGHT_32_END_STABILITY=5.6000 PRIMER_PAIR_32_COMPL_ANY=3.00 PRIMER_PAIR_32_COMPL_END=0.00 PRIMER_PAIR_32_PRODUCT_SIZE=98 PRIMER_PAIR_33_PENALTY=2.902052 PRIMER_LEFT_33_PENALTY=2.125665 PRIMER_RIGHT_33_PENALTY=0.776387 PRIMER_LEFT_33_SEQUENCE=GGATGGAGAGCCTGAGTTGG PRIMER_RIGHT_33_SEQUENCE=ACCTCGGCCAGGAAGAGTAG PRIMER_LEFT_33=183,20 PRIMER_RIGHT_33=728,20 PRIMER_LEFT_33_TM=62.126 PRIMER_RIGHT_33_TM=60.776 PRIMER_LEFT_33_GC_PERCENT=60.000 PRIMER_RIGHT_33_GC_PERCENT=60.000 PRIMER_LEFT_33_SELF_ANY=3.00 PRIMER_RIGHT_33_SELF_ANY=6.00 PRIMER_LEFT_33_SELF_END=0.00 PRIMER_RIGHT_33_SELF_END=0.00 PRIMER_LEFT_33_END_STABILITY=8.2000 PRIMER_RIGHT_33_END_STABILITY=5.4000 PRIMER_PAIR_33_COMPL_ANY=4.00 PRIMER_PAIR_33_COMPL_END=0.00 PRIMER_PAIR_33_PRODUCT_SIZE=546 PRIMER_PAIR_34_PENALTY=2.926857 PRIMER_LEFT_34_PENALTY=2.125665 PRIMER_RIGHT_34_PENALTY=0.801192 PRIMER_LEFT_34_SEQUENCE=GGGATGGAGAGCCTGAGTTG PRIMER_RIGHT_34_SEQUENCE=TCGCTGATGGAGTTCAGGTT PRIMER_LEFT_34=182,20 PRIMER_RIGHT_34=641,20 PRIMER_LEFT_34_TM=62.126 PRIMER_RIGHT_34_TM=60.801 PRIMER_LEFT_34_GC_PERCENT=60.000 PRIMER_RIGHT_34_GC_PERCENT=50.000 PRIMER_LEFT_34_SELF_ANY=3.00 PRIMER_RIGHT_34_SELF_ANY=4.00 PRIMER_LEFT_34_SELF_END=0.00 PRIMER_RIGHT_34_SELF_END=0.00 PRIMER_LEFT_34_END_STABILITY=6.7000 PRIMER_RIGHT_34_END_STABILITY=7.9000 PRIMER_PAIR_34_COMPL_ANY=5.00 PRIMER_PAIR_34_COMPL_END=3.00 PRIMER_PAIR_34_PRODUCT_SIZE=460 PRIMER_PAIR_35_PENALTY=2.938687 PRIMER_LEFT_35_PENALTY=2.125665 PRIMER_RIGHT_35_PENALTY=0.813021 PRIMER_LEFT_35_SEQUENCE=TGGGATGGAGAGCCTGAGTT PRIMER_RIGHT_35_SEQUENCE=CCCTGCTCAGGTAGAGCTTC PRIMER_LEFT_35=181,20 PRIMER_RIGHT_35=406,20 PRIMER_LEFT_35_TM=62.126 PRIMER_RIGHT_35_TM=59.187 PRIMER_LEFT_35_GC_PERCENT=55.000 PRIMER_RIGHT_35_GC_PERCENT=60.000 PRIMER_LEFT_35_SELF_ANY=3.00 PRIMER_RIGHT_35_SELF_ANY=6.00 PRIMER_LEFT_35_SELF_END=0.00 PRIMER_RIGHT_35_SELF_END=2.00 PRIMER_LEFT_35_END_STABILITY=6.4000 PRIMER_RIGHT_35_END_STABILITY=8.2000 PRIMER_PAIR_35_COMPL_ANY=6.00 PRIMER_PAIR_35_COMPL_END=1.00 PRIMER_PAIR_35_PRODUCT_SIZE=226 PRIMER_PAIR_36_PENALTY=2.975455 PRIMER_LEFT_36_PENALTY=2.141376 PRIMER_RIGHT_36_PENALTY=0.834079 PRIMER_LEFT_36_SEQUENCE=TGGGATGGAGAGCCTGAG PRIMER_RIGHT_36_SEQUENCE=GAGCGGTAGAAGTGGATGGT PRIMER_LEFT_36=181,18 PRIMER_RIGHT_36=896,20 PRIMER_LEFT_36_TM=59.859 PRIMER_RIGHT_36_TM=59.166 PRIMER_LEFT_36_GC_PERCENT=61.111 PRIMER_RIGHT_36_GC_PERCENT=55.000 PRIMER_LEFT_36_SELF_ANY=3.00 PRIMER_RIGHT_36_SELF_ANY=2.00 PRIMER_LEFT_36_SELF_END=3.00 PRIMER_RIGHT_36_SELF_END=0.00 PRIMER_LEFT_36_END_STABILITY=6.7000 PRIMER_RIGHT_36_END_STABILITY=7.8000 PRIMER_PAIR_36_COMPL_ANY=2.00 PRIMER_PAIR_36_COMPL_END=0.00 PRIMER_PAIR_36_PRODUCT_SIZE=716 PRIMER_PAIR_37_PENALTY=3.002161 PRIMER_LEFT_37_PENALTY=2.205825 PRIMER_RIGHT_37_PENALTY=0.796337 PRIMER_LEFT_37_SEQUENCE=GAAGTGCTTGGATGCGGT PRIMER_RIGHT_37_SEQUENCE=TACGAATGCCAACTCAGGCT PRIMER_LEFT_37=134,18 PRIMER_RIGHT_37=210,20 PRIMER_LEFT_37_TM=59.794 PRIMER_RIGHT_37_TM=60.796 PRIMER_LEFT_37_GC_PERCENT=55.556 PRIMER_RIGHT_37_GC_PERCENT=50.000 PRIMER_LEFT_37_SELF_ANY=4.00 PRIMER_RIGHT_37_SELF_ANY=3.00 PRIMER_LEFT_37_SELF_END=0.00 PRIMER_RIGHT_37_SELF_END=3.00 PRIMER_LEFT_37_END_STABILITY=11.1000 PRIMER_RIGHT_37_END_STABILITY=9.4000 PRIMER_PAIR_37_COMPL_ANY=5.00 PRIMER_PAIR_37_COMPL_END=0.00 PRIMER_PAIR_37_PRODUCT_SIZE=77 PRIMER_PAIR_38_PENALTY=3.007846 PRIMER_LEFT_38_PENALTY=2.146683 PRIMER_RIGHT_38_PENALTY=0.861164 PRIMER_LEFT_38_SEQUENCE=TGGCATTCGTATAAATGACCTG PRIMER_RIGHT_38_SEQUENCE=TTCAGGTTGTGGATGTTGCT PRIMER_LEFT_38=200,22 PRIMER_RIGHT_38=629,20 PRIMER_LEFT_38_TM=59.853 PRIMER_RIGHT_38_TM=59.139 PRIMER_LEFT_38_GC_PERCENT=40.909 PRIMER_RIGHT_38_GC_PERCENT=45.000 PRIMER_LEFT_38_SELF_ANY=6.00 PRIMER_RIGHT_38_SELF_ANY=2.00 PRIMER_LEFT_38_SELF_END=2.00 PRIMER_RIGHT_38_SELF_END=0.00 PRIMER_LEFT_38_END_STABILITY=7.9000 PRIMER_RIGHT_38_END_STABILITY=8.5000 PRIMER_PAIR_38_COMPL_ANY=5.00 PRIMER_PAIR_38_COMPL_END=0.00 PRIMER_PAIR_38_PRODUCT_SIZE=430 PRIMER_PAIR_39_PENALTY=3.037638 PRIMER_LEFT_39_PENALTY=2.153955 PRIMER_RIGHT_39_PENALTY=0.883683 PRIMER_LEFT_39_SEQUENCE=GATGGGATGGAGAGCCTGA PRIMER_RIGHT_39_SEQUENCE=GAACTTGATCCAGCAGAGCA PRIMER_LEFT_39=179,19 PRIMER_RIGHT_39=753,20 PRIMER_LEFT_39_TM=61.154 PRIMER_RIGHT_39_TM=59.116 PRIMER_LEFT_39_GC_PERCENT=57.895 PRIMER_RIGHT_39_GC_PERCENT=50.000 PRIMER_LEFT_39_SELF_ANY=3.00 PRIMER_RIGHT_39_SELF_ANY=6.00 PRIMER_LEFT_39_SELF_END=1.00 PRIMER_RIGHT_39_SELF_END=2.00 PRIMER_LEFT_39_END_STABILITY=8.2000 PRIMER_RIGHT_39_END_STABILITY=8.2000 PRIMER_PAIR_39_COMPL_ANY=4.00 PRIMER_PAIR_39_COMPL_END=1.00 PRIMER_PAIR_39_PRODUCT_SIZE=575 PRIMER_PAIR_40_PENALTY=3.081219 PRIMER_LEFT_40_PENALTY=2.188530 PRIMER_RIGHT_40_PENALTY=0.892689 PRIMER_LEFT_40_SEQUENCE=TAAATGACCTGCCTGGCTC PRIMER_RIGHT_40_SEQUENCE=CCGGTAATCCATGCCCTTAT PRIMER_LEFT_40=211,19 PRIMER_RIGHT_40=315,20 PRIMER_LEFT_40_TM=58.811 PRIMER_RIGHT_40_TM=60.893 PRIMER_LEFT_40_GC_PERCENT=52.632 PRIMER_RIGHT_40_GC_PERCENT=50.000 PRIMER_LEFT_40_SELF_ANY=5.00 PRIMER_RIGHT_40_SELF_ANY=4.00 PRIMER_LEFT_40_SELF_END=3.00 PRIMER_RIGHT_40_SELF_END=2.00 PRIMER_LEFT_40_END_STABILITY=9.4000 PRIMER_RIGHT_40_END_STABILITY=5.9000 PRIMER_PAIR_40_COMPL_ANY=4.00 PRIMER_PAIR_40_COMPL_END=1.00 PRIMER_PAIR_40_PRODUCT_SIZE=105 PRIMER_PAIR_41_PENALTY=3.193213 PRIMER_LEFT_41_PENALTY=2.205825 PRIMER_RIGHT_41_PENALTY=0.987388 PRIMER_LEFT_41_SEQUENCE=GCCTGAGTTGGCATTCGT PRIMER_RIGHT_41_SEQUENCE=AGTGGATGGTGAAGACCACG PRIMER_LEFT_41=192,18 PRIMER_RIGHT_41=886,20 PRIMER_LEFT_41_TM=59.794 PRIMER_RIGHT_41_TM=60.987 PRIMER_LEFT_41_GC_PERCENT=55.556 PRIMER_RIGHT_41_GC_PERCENT=55.000 PRIMER_LEFT_41_SELF_ANY=3.00 PRIMER_RIGHT_41_SELF_ANY=4.00 PRIMER_LEFT_41_SELF_END=0.00 PRIMER_RIGHT_41_SELF_END=2.00 PRIMER_LEFT_41_END_STABILITY=8.4000 PRIMER_RIGHT_41_END_STABILITY=9.9000 PRIMER_PAIR_41_COMPL_ANY=4.00 PRIMER_PAIR_41_COMPL_END=3.00 PRIMER_PAIR_41_PRODUCT_SIZE=695 PRIMER_PAIR_42_PENALTY=3.231013 PRIMER_LEFT_42_PENALTY=2.217968 PRIMER_RIGHT_42_PENALTY=1.013045 PRIMER_LEFT_42_SEQUENCE=GCTCCCACCATGAGTGCT PRIMER_RIGHT_42_SEQUENCE=GTTCAGGTTGTGGATGTTGC PRIMER_LEFT_42=226,18 PRIMER_RIGHT_42=630,20 PRIMER_LEFT_42_TM=59.782 PRIMER_RIGHT_42_TM=58.987 PRIMER_LEFT_42_GC_PERCENT=61.111 PRIMER_RIGHT_42_GC_PERCENT=50.000 PRIMER_LEFT_42_SELF_ANY=6.00 PRIMER_RIGHT_42_SELF_ANY=2.00 PRIMER_LEFT_42_SELF_END=2.00 PRIMER_RIGHT_42_SELF_END=2.00 PRIMER_LEFT_42_END_STABILITY=7.9000 PRIMER_RIGHT_42_END_STABILITY=8.2000 PRIMER_PAIR_42_COMPL_ANY=5.00 PRIMER_PAIR_42_COMPL_END=1.00 PRIMER_PAIR_42_PRODUCT_SIZE=405 PRIMER_PAIR_43_PENALTY=3.268525 PRIMER_LEFT_43_PENALTY=2.250013 PRIMER_RIGHT_43_PENALTY=1.018512 PRIMER_LEFT_43_SEQUENCE=GGATGTGCGCAAGGAGAT PRIMER_RIGHT_43_SEQUENCE=ATGGTGAAGACCACGAAGATG PRIMER_LEFT_43=164,18 PRIMER_RIGHT_43=881,21 PRIMER_LEFT_43_TM=59.750 PRIMER_RIGHT_43_TM=59.981 PRIMER_LEFT_43_GC_PERCENT=55.556 PRIMER_RIGHT_43_GC_PERCENT=47.619 PRIMER_LEFT_43_SELF_ANY=6.00 PRIMER_RIGHT_43_SELF_ANY=4.00 PRIMER_LEFT_43_SELF_END=2.00 PRIMER_RIGHT_43_SELF_END=0.00 PRIMER_LEFT_43_END_STABILITY=6.3000 PRIMER_RIGHT_43_END_STABILITY=6.6000 PRIMER_PAIR_43_COMPL_ANY=3.00 PRIMER_PAIR_43_COMPL_END=1.00 PRIMER_PAIR_43_PRODUCT_SIZE=718 PRIMER_PAIR_44_PENALTY=3.287655 PRIMER_LEFT_44_PENALTY=2.441275 PRIMER_RIGHT_44_PENALTY=0.846380 PRIMER_LEFT_44_SEQUENCE=GGGATGGAAGTGCTTGGA PRIMER_RIGHT_44_SEQUENCE=GAGCCAGGCAGGTCATTTAT PRIMER_LEFT_44=128,18 PRIMER_RIGHT_44=229,20 PRIMER_LEFT_44_TM=59.559 PRIMER_RIGHT_44_TM=59.154 PRIMER_LEFT_44_GC_PERCENT=55.556 PRIMER_RIGHT_44_GC_PERCENT=50.000 PRIMER_LEFT_44_SELF_ANY=4.00 PRIMER_RIGHT_44_SELF_ANY=5.00 PRIMER_LEFT_44_SELF_END=2.00 PRIMER_RIGHT_44_SELF_END=2.00 PRIMER_LEFT_44_END_STABILITY=8.5000 PRIMER_RIGHT_44_END_STABILITY=6.2000 PRIMER_PAIR_44_COMPL_ANY=5.00 PRIMER_PAIR_44_COMPL_END=1.00 PRIMER_PAIR_44_PRODUCT_SIZE=102 PRIMER_PAIR_45_PENALTY=3.305926 PRIMER_LEFT_45_PENALTY=2.278334 PRIMER_RIGHT_45_PENALTY=1.027592 PRIMER_LEFT_45_SEQUENCE=GATGGAGAGCCTGAGTTGGC PRIMER_RIGHT_45_SEQUENCE=GTTCAGGTTGTGGATGTTGCT PRIMER_LEFT_45=184,20 PRIMER_RIGHT_45=630,21 PRIMER_LEFT_45_TM=62.278 PRIMER_RIGHT_45_TM=60.028 PRIMER_LEFT_45_GC_PERCENT=60.000 PRIMER_RIGHT_45_GC_PERCENT=47.619 PRIMER_LEFT_45_SELF_ANY=3.00 PRIMER_RIGHT_45_SELF_ANY=2.00 PRIMER_LEFT_45_SELF_END=2.00 PRIMER_RIGHT_45_SELF_END=0.00 PRIMER_LEFT_45_END_STABILITY=10.0000 PRIMER_RIGHT_45_END_STABILITY=8.5000 PRIMER_PAIR_45_COMPL_ANY=5.00 PRIMER_PAIR_45_COMPL_END=1.00 PRIMER_PAIR_45_PRODUCT_SIZE=447 PRIMER_PAIR_46_PENALTY=3.456958 PRIMER_LEFT_46_PENALTY=2.429366 PRIMER_RIGHT_46_PENALTY=1.027592 PRIMER_LEFT_46_SEQUENCE=TAAATGACCTGCCTGGCTCC PRIMER_RIGHT_46_SEQUENCE=AGTTCAGGTTGTGGATGTTGC PRIMER_LEFT_46=211,20 PRIMER_RIGHT_46=631,21 PRIMER_LEFT_46_TM=62.429 PRIMER_RIGHT_46_TM=60.028 PRIMER_LEFT_46_GC_PERCENT=55.000 PRIMER_RIGHT_46_GC_PERCENT=47.619 PRIMER_LEFT_46_SELF_ANY=5.00 PRIMER_RIGHT_46_SELF_ANY=2.00 PRIMER_LEFT_46_SELF_END=2.00 PRIMER_RIGHT_46_SELF_END=2.00 PRIMER_LEFT_46_END_STABILITY=9.4000 PRIMER_RIGHT_46_END_STABILITY=8.2000 PRIMER_PAIR_46_COMPL_ANY=5.00 PRIMER_PAIR_46_COMPL_END=1.00 PRIMER_PAIR_46_PRODUCT_SIZE=421 PRIMER_PAIR_47_PENALTY=3.528337 PRIMER_LEFT_47_PENALTY=2.481969 PRIMER_RIGHT_47_PENALTY=1.046368 PRIMER_LEFT_47_SEQUENCE=GCAAGGAGATGGGATGGAGA PRIMER_RIGHT_47_SEQUENCE=CGATAGGCACGTTAAGCTCAG PRIMER_LEFT_47=172,20 PRIMER_RIGHT_47=262,21 PRIMER_LEFT_47_TM=62.482 PRIMER_RIGHT_47_TM=60.046 PRIMER_LEFT_47_GC_PERCENT=55.000 PRIMER_RIGHT_47_GC_PERCENT=52.381 PRIMER_LEFT_47_SELF_ANY=2.00 PRIMER_RIGHT_47_SELF_ANY=4.00 PRIMER_LEFT_47_SELF_END=0.00 PRIMER_RIGHT_47_SELF_END=3.00 PRIMER_LEFT_47_END_STABILITY=7.9000 PRIMER_RIGHT_47_END_STABILITY=6.7000 PRIMER_PAIR_47_COMPL_ANY=3.00 PRIMER_PAIR_47_COMPL_END=0.00 PRIMER_PAIR_47_PRODUCT_SIZE=91 PRIMER_PAIR_48_PENALTY=3.533715 PRIMER_LEFT_48_PENALTY=2.484888 PRIMER_RIGHT_48_PENALTY=1.048827 PRIMER_LEFT_48_SEQUENCE=CAAGGAGATGGGATGGAGA PRIMER_RIGHT_48_SEQUENCE=CTCGGCCAGGAAGAGTAGG PRIMER_LEFT_48=173,19 PRIMER_RIGHT_48=726,19 PRIMER_LEFT_48_TM=58.515 PRIMER_RIGHT_48_TM=59.951 PRIMER_LEFT_48_GC_PERCENT=52.632 PRIMER_RIGHT_48_GC_PERCENT=63.158 PRIMER_LEFT_48_SELF_ANY=2.00 PRIMER_RIGHT_48_SELF_ANY=5.00 PRIMER_LEFT_48_SELF_END=0.00 PRIMER_RIGHT_48_SELF_END=0.00 PRIMER_LEFT_48_END_STABILITY=7.9000 PRIMER_RIGHT_48_END_STABILITY=6.9000 PRIMER_PAIR_48_COMPL_ANY=3.00 PRIMER_PAIR_48_COMPL_END=0.00 PRIMER_PAIR_48_PRODUCT_SIZE=554 PRIMER_PAIR_49_PENALTY=3.537252 PRIMER_LEFT_49_PENALTY=2.488425 PRIMER_RIGHT_49_PENALTY=1.048827 PRIMER_LEFT_49_SEQUENCE=GCAAGGAGATGGGATGGA PRIMER_RIGHT_49_SEQUENCE=CCTCGGCCAGGAAGAGTAG PRIMER_LEFT_49=172,18 PRIMER_RIGHT_49=727,19 PRIMER_LEFT_49_TM=59.512 PRIMER_RIGHT_49_TM=59.951 PRIMER_LEFT_49_GC_PERCENT=55.556 PRIMER_RIGHT_49_GC_PERCENT=63.158 PRIMER_LEFT_49_SELF_ANY=2.00 PRIMER_RIGHT_49_SELF_ANY=6.00 PRIMER_LEFT_49_SELF_END=0.00 PRIMER_RIGHT_49_SELF_END=0.00 PRIMER_LEFT_49_END_STABILITY=8.1000 PRIMER_RIGHT_49_END_STABILITY=5.4000 PRIMER_PAIR_49_COMPL_ANY=3.00 PRIMER_PAIR_49_COMPL_END=0.00 PRIMER_PAIR_49_PRODUCT_SIZE=556 PRIMER_PAIR_50_PENALTY=3.666838 PRIMER_LEFT_50_PENALTY=2.613581 PRIMER_RIGHT_50_PENALTY=1.053256 PRIMER_LEFT_50_SEQUENCE=CAGGGATGGAAGTGCTTGG PRIMER_RIGHT_50_SEQUENCE=ATAGGCACGTTAAGCTCAGCA PRIMER_LEFT_50=126,19 PRIMER_RIGHT_50=260,21 PRIMER_LEFT_50_TM=61.614 PRIMER_RIGHT_50_TM=60.053 PRIMER_LEFT_50_GC_PERCENT=57.895 PRIMER_RIGHT_50_GC_PERCENT=47.619 PRIMER_LEFT_50_SELF_ANY=4.00 PRIMER_RIGHT_50_SELF_ANY=5.00 PRIMER_LEFT_50_SELF_END=0.00 PRIMER_RIGHT_50_SELF_END=3.00 PRIMER_LEFT_50_END_STABILITY=8.5000 PRIMER_RIGHT_50_END_STABILITY=8.5000 PRIMER_PAIR_50_COMPL_ANY=4.00 PRIMER_PAIR_50_COMPL_END=3.00 PRIMER_PAIR_50_PRODUCT_SIZE=135 PRIMER_PAIR_51_PENALTY=3.668226 PRIMER_LEFT_51_PENALTY=2.613581 PRIMER_RIGHT_51_PENALTY=1.054645 PRIMER_LEFT_51_SEQUENCE=CCAGGGATGGAAGTGCTTG PRIMER_RIGHT_51_SEQUENCE=GTGGATGGTGAAGACCACG PRIMER_LEFT_51=125,19 PRIMER_RIGHT_51=885,19 PRIMER_LEFT_51_TM=61.614 PRIMER_RIGHT_51_TM=59.945 PRIMER_LEFT_51_GC_PERCENT=57.895 PRIMER_RIGHT_51_GC_PERCENT=57.895 PRIMER_LEFT_51_SELF_ANY=4.00 PRIMER_RIGHT_51_SELF_ANY=4.00 PRIMER_LEFT_51_SELF_END=2.00 PRIMER_RIGHT_51_SELF_END=2.00 PRIMER_LEFT_51_END_STABILITY=8.5000 PRIMER_RIGHT_51_END_STABILITY=9.9000 PRIMER_PAIR_51_COMPL_ANY=3.00 PRIMER_PAIR_51_COMPL_END=2.00 PRIMER_PAIR_51_PRODUCT_SIZE=761 PRIMER_PAIR_52_PENALTY=3.722775 PRIMER_LEFT_52_PENALTY=2.665121 PRIMER_RIGHT_52_PENALTY=1.057654 PRIMER_LEFT_52_SEQUENCE=TATAAATGACCTGCCTGGCT PRIMER_RIGHT_52_SEQUENCE=GCCCTGCTCAGGTAGAGCTT PRIMER_LEFT_52=209,20 PRIMER_RIGHT_52=407,20 PRIMER_LEFT_52_TM=57.335 PRIMER_RIGHT_52_TM=61.058 PRIMER_LEFT_52_GC_PERCENT=45.000 PRIMER_RIGHT_52_GC_PERCENT=60.000 PRIMER_LEFT_52_SELF_ANY=5.00 PRIMER_RIGHT_52_SELF_ANY=6.00 PRIMER_LEFT_52_SELF_END=3.00 PRIMER_RIGHT_52_SELF_END=2.00 PRIMER_LEFT_52_END_STABILITY=9.7000 PRIMER_RIGHT_52_END_STABILITY=8.2000 PRIMER_PAIR_52_COMPL_ANY=5.00 PRIMER_PAIR_52_COMPL_END=3.00 PRIMER_PAIR_52_PRODUCT_SIZE=199 PRIMER_PAIR_53_PENALTY=3.746319 PRIMER_LEFT_53_PENALTY=2.679749 PRIMER_RIGHT_53_PENALTY=1.066570 PRIMER_LEFT_53_SEQUENCE=GAGCCTGAGTTGGCATTCGT PRIMER_RIGHT_53_SEQUENCE=GCGGTAGAAGTGGATGGTGA PRIMER_LEFT_53=190,20 PRIMER_RIGHT_53=894,20 PRIMER_LEFT_53_TM=62.680 PRIMER_RIGHT_53_TM=61.067 PRIMER_LEFT_53_GC_PERCENT=55.000 PRIMER_RIGHT_53_GC_PERCENT=55.000 PRIMER_LEFT_53_SELF_ANY=3.00 PRIMER_RIGHT_53_SELF_ANY=2.00 PRIMER_LEFT_53_SELF_END=0.00 PRIMER_RIGHT_53_SELF_END=1.00 PRIMER_LEFT_53_END_STABILITY=8.4000 PRIMER_RIGHT_53_END_STABILITY=7.9000 PRIMER_PAIR_53_COMPL_ANY=3.00 PRIMER_PAIR_53_COMPL_END=1.00 PRIMER_PAIR_53_PRODUCT_SIZE=705 PRIMER_PAIR_54_PENALTY=3.797786 PRIMER_LEFT_54_PENALTY=2.694261 PRIMER_RIGHT_54_PENALTY=1.103525 PRIMER_LEFT_54_SEQUENCE=GATGGAGAGCCTGAGTTGG PRIMER_RIGHT_54_SEQUENCE=CTCAGGTAGAGCTTCCGCC PRIMER_LEFT_54=184,19 PRIMER_RIGHT_54=401,19 PRIMER_LEFT_54_TM=58.306 PRIMER_RIGHT_54_TM=60.104 PRIMER_LEFT_54_GC_PERCENT=57.895 PRIMER_RIGHT_54_GC_PERCENT=63.158 PRIMER_LEFT_54_SELF_ANY=3.00 PRIMER_RIGHT_54_SELF_ANY=4.00 PRIMER_LEFT_54_SELF_END=0.00 PRIMER_RIGHT_54_SELF_END=2.00 PRIMER_LEFT_54_END_STABILITY=8.2000 PRIMER_RIGHT_54_END_STABILITY=12.9000 PRIMER_PAIR_54_COMPL_ANY=6.00 PRIMER_PAIR_54_COMPL_END=2.00 PRIMER_PAIR_54_PRODUCT_SIZE=218 PRIMER_PAIR_55_PENALTY=3.805118 PRIMER_LEFT_55_PENALTY=2.694261 PRIMER_RIGHT_55_PENALTY=1.110857 PRIMER_LEFT_55_SEQUENCE=GGATGGAGAGCCTGAGTTG PRIMER_RIGHT_55_SEQUENCE=GGAAGAGTAGGATGCCAAGC PRIMER_LEFT_55=183,19 PRIMER_RIGHT_55=718,20 PRIMER_LEFT_55_TM=58.306 PRIMER_RIGHT_55_TM=58.889 PRIMER_LEFT_55_GC_PERCENT=57.895 PRIMER_RIGHT_55_GC_PERCENT=55.000 PRIMER_LEFT_55_SELF_ANY=3.00 PRIMER_RIGHT_55_SELF_ANY=3.00 PRIMER_LEFT_55_SELF_END=0.00 PRIMER_RIGHT_55_SELF_END=2.00 PRIMER_LEFT_55_END_STABILITY=6.7000 PRIMER_RIGHT_55_END_STABILITY=8.5000 PRIMER_PAIR_55_COMPL_ANY=3.00 PRIMER_PAIR_55_COMPL_END=2.00 PRIMER_PAIR_55_PRODUCT_SIZE=536 PRIMER_PAIR_56_PENALTY=3.814970 PRIMER_LEFT_56_PENALTY=2.700295 PRIMER_RIGHT_56_PENALTY=1.114674 PRIMER_LEFT_56_SEQUENCE=GCATTCGTATAAATGACCTGCC PRIMER_RIGHT_56_SEQUENCE=TCGGCCAGGAAGAGTAGGAT PRIMER_LEFT_56=202,22 PRIMER_RIGHT_56=725,20 PRIMER_LEFT_56_TM=60.700 PRIMER_RIGHT_56_TM=61.115 PRIMER_LEFT_56_GC_PERCENT=45.455 PRIMER_RIGHT_56_GC_PERCENT=55.000 PRIMER_LEFT_56_SELF_ANY=6.00 PRIMER_RIGHT_56_SELF_ANY=4.00 PRIMER_LEFT_56_SELF_END=2.00 PRIMER_RIGHT_56_SELF_END=2.00 PRIMER_LEFT_56_END_STABILITY=9.7000 PRIMER_RIGHT_56_END_STABILITY=7.8000 PRIMER_PAIR_56_COMPL_ANY=4.00 PRIMER_PAIR_56_COMPL_END=1.00 PRIMER_PAIR_56_PRODUCT_SIZE=524 PRIMER_PAIR_57_PENALTY=3.836405 PRIMER_LEFT_57_PENALTY=2.726528 PRIMER_RIGHT_57_PENALTY=1.109878 PRIMER_LEFT_57_SEQUENCE=ACGTCCCAGGGATGGAAGT PRIMER_RIGHT_57_SEQUENCE=TATACGAATGCCAACTCAGGC PRIMER_LEFT_57=120,19 PRIMER_RIGHT_57=212,21 PRIMER_LEFT_57_TM=61.727 PRIMER_RIGHT_57_TM=60.110 PRIMER_LEFT_57_GC_PERCENT=57.895 PRIMER_RIGHT_57_GC_PERCENT=47.619 PRIMER_LEFT_57_SELF_ANY=7.00 PRIMER_RIGHT_57_SELF_ANY=4.00 PRIMER_LEFT_57_SELF_END=2.00 PRIMER_RIGHT_57_SELF_END=2.00 PRIMER_LEFT_57_END_STABILITY=6.4000 PRIMER_RIGHT_57_END_STABILITY=9.7000 PRIMER_PAIR_57_COMPL_ANY=4.00 PRIMER_PAIR_57_COMPL_END=3.00 PRIMER_PAIR_57_PRODUCT_SIZE=93 PRIMER_PAIR_58_PENALTY=3.874414 PRIMER_LEFT_58_PENALTY=2.748413 PRIMER_RIGHT_58_PENALTY=1.126001 PRIMER_LEFT_58_SEQUENCE=GGGATGGAAGTGCTTGGATG PRIMER_RIGHT_58_SEQUENCE=GCCAGGAAGAGTAGGATGCC PRIMER_LEFT_58=128,20 PRIMER_RIGHT_58=722,20 PRIMER_LEFT_58_TM=62.748 PRIMER_RIGHT_58_TM=61.126 PRIMER_LEFT_58_GC_PERCENT=55.000 PRIMER_RIGHT_58_GC_PERCENT=60.000 PRIMER_LEFT_58_SELF_ANY=4.00 PRIMER_RIGHT_58_SELF_ANY=3.00 PRIMER_LEFT_58_SELF_END=2.00 PRIMER_RIGHT_58_SELF_END=2.00 PRIMER_LEFT_58_END_STABILITY=8.1000 PRIMER_RIGHT_58_END_STABILITY=9.6000 PRIMER_PAIR_58_COMPL_ANY=3.00 PRIMER_PAIR_58_COMPL_END=1.00 PRIMER_PAIR_58_PRODUCT_SIZE=595 PRIMER_PAIR_59_PENALTY=3.900013 PRIMER_LEFT_59_PENALTY=2.774013 PRIMER_RIGHT_59_PENALTY=1.126001 PRIMER_LEFT_59_SEQUENCE=GTATAAATGACCTGCCTGGCT PRIMER_RIGHT_59_SEQUENCE=GGCCAGGAAGAGTAGGATGC PRIMER_LEFT_59=208,21 PRIMER_RIGHT_59=723,20 PRIMER_LEFT_59_TM=58.226 PRIMER_RIGHT_59_TM=61.126 PRIMER_LEFT_59_GC_PERCENT=47.619 PRIMER_RIGHT_59_GC_PERCENT=60.000 PRIMER_LEFT_59_SELF_ANY=5.00 PRIMER_RIGHT_59_SELF_ANY=4.00 PRIMER_LEFT_59_SELF_END=3.00 PRIMER_RIGHT_59_SELF_END=2.00 PRIMER_LEFT_59_END_STABILITY=9.7000 PRIMER_RIGHT_59_END_STABILITY=8.1000 PRIMER_PAIR_59_COMPL_ANY=7.00 PRIMER_PAIR_59_COMPL_END=3.00 PRIMER_PAIR_59_PRODUCT_SIZE=516 PRIMER_PAIR_60_PENALTY=3.941862 PRIMER_LEFT_60_PENALTY=2.792938 PRIMER_RIGHT_60_PENALTY=1.148923 PRIMER_LEFT_60_SEQUENCE=AGAGCCTGAGTTGGCATTCG PRIMER_RIGHT_60_SEQUENCE=GAACTTGATCCAGCAGAGCAG PRIMER_LEFT_60=189,20 PRIMER_RIGHT_60=753,21 PRIMER_LEFT_60_TM=62.793 PRIMER_RIGHT_60_TM=60.149 PRIMER_LEFT_60_GC_PERCENT=55.000 PRIMER_RIGHT_60_GC_PERCENT=52.381 PRIMER_LEFT_60_SELF_ANY=3.00 PRIMER_RIGHT_60_SELF_ANY=6.00 PRIMER_LEFT_60_SELF_END=2.00 PRIMER_RIGHT_60_SELF_END=1.00 PRIMER_LEFT_60_END_STABILITY=8.6000 PRIMER_RIGHT_60_END_STABILITY=8.2000 PRIMER_PAIR_60_COMPL_ANY=4.00 PRIMER_PAIR_60_COMPL_END=0.00 PRIMER_PAIR_60_PRODUCT_SIZE=565 PRIMER_PAIR_61_PENALTY=3.943966 PRIMER_LEFT_61_PENALTY=2.806404 PRIMER_RIGHT_61_PENALTY=1.137562 PRIMER_LEFT_61_SEQUENCE=ATGTGCGCAAGGAGATGG PRIMER_RIGHT_61_SEQUENCE=AGGAAGAGTAGGATGCCAAGC PRIMER_LEFT_61=166,18 PRIMER_RIGHT_61=719,21 PRIMER_LEFT_61_TM=60.806 PRIMER_RIGHT_61_TM=59.862 PRIMER_LEFT_61_GC_PERCENT=55.556 PRIMER_RIGHT_61_GC_PERCENT=52.381 PRIMER_LEFT_61_SELF_ANY=6.00 PRIMER_RIGHT_61_SELF_ANY=3.00 PRIMER_LEFT_61_SELF_END=0.00 PRIMER_RIGHT_61_SELF_END=2.00 PRIMER_LEFT_61_END_STABILITY=8.1000 PRIMER_RIGHT_61_END_STABILITY=8.5000 PRIMER_PAIR_61_COMPL_ANY=3.00 PRIMER_PAIR_61_COMPL_END=1.00 PRIMER_PAIR_61_PRODUCT_SIZE=554 PRIMER_PAIR_62_PENALTY=3.992475 PRIMER_LEFT_62_PENALTY=2.827930 PRIMER_RIGHT_62_PENALTY=1.164545 PRIMER_LEFT_62_SEQUENCE=GGAGATGGGATGGAGAGCCT PRIMER_RIGHT_62_SEQUENCE=ATGGAGTTCAGGTTGTGGATG PRIMER_LEFT_62=176,20 PRIMER_RIGHT_62=635,21 PRIMER_LEFT_62_TM=62.828 PRIMER_RIGHT_62_TM=59.835 PRIMER_LEFT_62_GC_PERCENT=60.000 PRIMER_RIGHT_62_GC_PERCENT=47.619 PRIMER_LEFT_62_SELF_ANY=3.00 PRIMER_RIGHT_62_SELF_ANY=3.00 PRIMER_LEFT_62_SELF_END=3.00 PRIMER_RIGHT_62_SELF_END=0.00 PRIMER_LEFT_62_END_STABILITY=9.4000 PRIMER_RIGHT_62_END_STABILITY=8.1000 PRIMER_PAIR_62_COMPL_ANY=3.00 PRIMER_PAIR_62_COMPL_END=2.00 PRIMER_PAIR_62_PRODUCT_SIZE=460 PRIMER_PAIR_63_PENALTY=3.997588 PRIMER_LEFT_63_PENALTY=2.827930 PRIMER_RIGHT_63_PENALTY=1.169658 PRIMER_LEFT_63_SEQUENCE=AGGAGATGGGATGGAGAGCC PRIMER_RIGHT_63_SEQUENCE=GCACGTTAAGCTCAGCACTC PRIMER_LEFT_63=175,20 PRIMER_RIGHT_63=256,20 PRIMER_LEFT_63_TM=62.828 PRIMER_RIGHT_63_TM=58.830 PRIMER_LEFT_63_GC_PERCENT=60.000 PRIMER_RIGHT_63_GC_PERCENT=55.000 PRIMER_LEFT_63_SELF_ANY=2.00 PRIMER_RIGHT_63_SELF_ANY=5.00 PRIMER_LEFT_63_SELF_END=1.00 PRIMER_RIGHT_63_SELF_END=0.00 PRIMER_LEFT_63_END_STABILITY=9.4000 PRIMER_RIGHT_63_END_STABILITY=6.4000 PRIMER_PAIR_63_COMPL_ANY=4.00 PRIMER_PAIR_63_COMPL_END=3.00 PRIMER_PAIR_63_PRODUCT_SIZE=82 PRIMER_PAIR_64_PENALTY=4.011724 PRIMER_LEFT_64_PENALTY=2.840217 PRIMER_RIGHT_64_PENALTY=1.171507 PRIMER_LEFT_64_SEQUENCE=AAGTGCTTGGATGCGGTG PRIMER_RIGHT_64_SEQUENCE=CTTGATCCAGCAGAGCAGC PRIMER_LEFT_64=135,18 PRIMER_RIGHT_64=750,19 PRIMER_LEFT_64_TM=60.840 PRIMER_RIGHT_64_TM=59.828 PRIMER_LEFT_64_GC_PERCENT=55.556 PRIMER_RIGHT_64_GC_PERCENT=57.895 PRIMER_LEFT_64_SELF_ANY=4.00 PRIMER_RIGHT_64_SELF_ANY=6.00 PRIMER_LEFT_64_SELF_END=0.00 PRIMER_RIGHT_64_SELF_END=3.00 PRIMER_LEFT_64_END_STABILITY=9.9000 PRIMER_RIGHT_64_END_STABILITY=9.7000 PRIMER_PAIR_64_COMPL_ANY=6.00 PRIMER_PAIR_64_COMPL_END=3.00 PRIMER_PAIR_64_PRODUCT_SIZE=616 PRIMER_PAIR_65_PENALTY=4.042943 PRIMER_LEFT_65_PENALTY=2.857001 PRIMER_RIGHT_65_PENALTY=1.185942 PRIMER_LEFT_65_SEQUENCE=CCTGAGTTGGCATTCGTATAAA PRIMER_RIGHT_65_SEQUENCE=GGTACTGGTACTGCGTCTCCA PRIMER_LEFT_65=193,22 PRIMER_RIGHT_65=502,21 PRIMER_LEFT_65_TM=59.143 PRIMER_RIGHT_65_TM=60.186 PRIMER_LEFT_65_GC_PERCENT=40.909 PRIMER_RIGHT_65_GC_PERCENT=57.143 PRIMER_LEFT_65_SELF_ANY=4.00 PRIMER_RIGHT_65_SELF_ANY=6.00 PRIMER_LEFT_65_SELF_END=2.00 PRIMER_RIGHT_65_SELF_END=2.00 PRIMER_LEFT_65_END_STABILITY=6.2000 PRIMER_RIGHT_65_END_STABILITY=8.2000 PRIMER_PAIR_65_COMPL_ANY=3.00 PRIMER_PAIR_65_COMPL_END=0.00 PRIMER_PAIR_65_PRODUCT_SIZE=310 PRIMER_PAIR_66_PENALTY=4.075959 PRIMER_LEFT_66_PENALTY=2.890161 PRIMER_RIGHT_66_PENALTY=1.185797 PRIMER_LEFT_66_SEQUENCE=CGTCCCAGGGATGGAAGT PRIMER_RIGHT_66_SEQUENCE=TACGAATGCCAACTCAGGC PRIMER_LEFT_66=121,18 PRIMER_RIGHT_66=210,19 PRIMER_LEFT_66_TM=60.890 PRIMER_RIGHT_66_TM=59.814 PRIMER_LEFT_66_GC_PERCENT=61.111 PRIMER_RIGHT_66_GC_PERCENT=52.632 PRIMER_LEFT_66_SELF_ANY=7.00 PRIMER_RIGHT_66_SELF_ANY=3.00 PRIMER_LEFT_66_SELF_END=1.00 PRIMER_RIGHT_66_SELF_END=2.00 PRIMER_LEFT_66_END_STABILITY=6.4000 PRIMER_RIGHT_66_END_STABILITY=9.7000 PRIMER_PAIR_66_COMPL_ANY=4.00 PRIMER_PAIR_66_COMPL_END=3.00 PRIMER_PAIR_66_PRODUCT_SIZE=90 PRIMER_PAIR_67_PENALTY=4.078421 PRIMER_LEFT_67_PENALTY=2.890161 PRIMER_RIGHT_67_PENALTY=1.188260 PRIMER_LEFT_67_SEQUENCE=ACGTCCCAGGGATGGAAG PRIMER_RIGHT_67_SEQUENCE=ACCTCGGCCAGGAAGAGTA PRIMER_LEFT_67=120,18 PRIMER_RIGHT_67=728,19 PRIMER_LEFT_67_TM=60.890 PRIMER_RIGHT_67_TM=59.812 PRIMER_LEFT_67_GC_PERCENT=61.111 PRIMER_RIGHT_67_GC_PERCENT=57.895 PRIMER_LEFT_67_SELF_ANY=7.00 PRIMER_RIGHT_67_SELF_ANY=6.00 PRIMER_LEFT_67_SELF_END=0.00 PRIMER_RIGHT_67_SELF_END=2.00 PRIMER_LEFT_67_END_STABILITY=8.2000 PRIMER_RIGHT_67_END_STABILITY=5.4000 PRIMER_PAIR_67_COMPL_ANY=4.00 PRIMER_PAIR_67_COMPL_END=0.00 PRIMER_PAIR_67_PRODUCT_SIZE=609 PRIMER_PAIR_68_PENALTY=4.100063 PRIMER_LEFT_68_PENALTY=2.911803 PRIMER_RIGHT_68_PENALTY=1.188260 PRIMER_LEFT_68_SEQUENCE=GAGCCTGAGTTGGCATTCG PRIMER_RIGHT_68_SEQUENCE=AGGGAGCGGTAGAAGTGGA PRIMER_LEFT_68=190,19 PRIMER_RIGHT_68=899,19 PRIMER_LEFT_68_TM=61.912 PRIMER_RIGHT_68_TM=59.812 PRIMER_LEFT_68_GC_PERCENT=57.895 PRIMER_RIGHT_68_GC_PERCENT=57.895 PRIMER_LEFT_68_SELF_ANY=3.00 PRIMER_RIGHT_68_SELF_ANY=2.00 PRIMER_LEFT_68_SELF_END=2.00 PRIMER_RIGHT_68_SELF_END=0.00 PRIMER_LEFT_68_END_STABILITY=8.6000 PRIMER_RIGHT_68_END_STABILITY=7.9000 PRIMER_PAIR_68_COMPL_ANY=3.00 PRIMER_PAIR_68_COMPL_END=1.00 PRIMER_PAIR_68_PRODUCT_SIZE=710 PRIMER_PAIR_69_PENALTY=4.123274 PRIMER_LEFT_69_PENALTY=2.922864 PRIMER_RIGHT_69_PENALTY=1.200410 PRIMER_LEFT_69_SEQUENCE=ATGGGATGGAGAGCCTGA PRIMER_RIGHT_69_SEQUENCE=TGAAGACCACGAAGATGAGG PRIMER_LEFT_69=180,18 PRIMER_RIGHT_69=877,20 PRIMER_LEFT_69_TM=59.077 PRIMER_RIGHT_69_TM=58.800 PRIMER_LEFT_69_GC_PERCENT=55.556 PRIMER_RIGHT_69_GC_PERCENT=50.000 PRIMER_LEFT_69_SELF_ANY=3.00 PRIMER_RIGHT_69_SELF_ANY=2.00 PRIMER_LEFT_69_SELF_END=1.00 PRIMER_RIGHT_69_SELF_END=0.00 PRIMER_LEFT_69_END_STABILITY=8.2000 PRIMER_RIGHT_69_END_STABILITY=8.2000 PRIMER_PAIR_69_COMPL_ANY=3.00 PRIMER_PAIR_69_COMPL_END=3.00 PRIMER_PAIR_69_PRODUCT_SIZE=698 PRIMER_PAIR_70_PENALTY=4.138152 PRIMER_LEFT_70_PENALTY=2.922864 PRIMER_RIGHT_70_PENALTY=1.215288 PRIMER_LEFT_70_SEQUENCE=GATGGGATGGAGAGCCTG PRIMER_RIGHT_70_SEQUENCE=GGGACTCGCTGATGGAGTT PRIMER_LEFT_70=179,18 PRIMER_RIGHT_70=646,19 PRIMER_LEFT_70_TM=59.077 PRIMER_RIGHT_70_TM=60.215 PRIMER_LEFT_70_GC_PERCENT=61.111 PRIMER_RIGHT_70_GC_PERCENT=57.895 PRIMER_LEFT_70_SELF_ANY=3.00 PRIMER_RIGHT_70_SELF_ANY=5.00 PRIMER_LEFT_70_SELF_END=1.00 PRIMER_RIGHT_70_SELF_END=3.00 PRIMER_LEFT_70_END_STABILITY=9.7000 PRIMER_RIGHT_70_END_STABILITY=6.4000 PRIMER_PAIR_70_COMPL_ANY=3.00 PRIMER_PAIR_70_COMPL_END=0.00 PRIMER_PAIR_70_PRODUCT_SIZE=468 PRIMER_PAIR_71_PENALTY=4.173923 PRIMER_LEFT_71_PENALTY=2.944273 PRIMER_RIGHT_71_PENALTY=1.229650 PRIMER_LEFT_71_SEQUENCE=GGAGATGGGATGGAGAGCC PRIMER_RIGHT_71_SEQUENCE=GAGCAGAGGGGTCGATAGG PRIMER_LEFT_71=176,19 PRIMER_RIGHT_71=274,19 PRIMER_LEFT_71_TM=61.944 PRIMER_RIGHT_71_TM=59.770 PRIMER_LEFT_71_GC_PERCENT=63.158 PRIMER_RIGHT_71_GC_PERCENT=63.158 PRIMER_LEFT_71_SELF_ANY=2.00 PRIMER_RIGHT_71_SELF_ANY=4.00 PRIMER_LEFT_71_SELF_END=1.00 PRIMER_RIGHT_71_SELF_END=0.00 PRIMER_LEFT_71_END_STABILITY=9.4000 PRIMER_RIGHT_71_END_STABILITY=7.1000 PRIMER_PAIR_71_COMPL_ANY=3.00 PRIMER_PAIR_71_COMPL_END=2.00 PRIMER_PAIR_71_PRODUCT_SIZE=99 PRIMER_PAIR_72_PENALTY=4.195505 PRIMER_LEFT_72_PENALTY=2.965855 PRIMER_RIGHT_72_PENALTY=1.229650 PRIMER_LEFT_72_SEQUENCE=AACGTCCCAGGGATGGAAGT PRIMER_RIGHT_72_SEQUENCE=GGAGCAGAGGGGTCGATAG PRIMER_LEFT_72=119,20 PRIMER_RIGHT_72=275,19 PRIMER_LEFT_72_TM=62.966 PRIMER_RIGHT_72_TM=59.770 PRIMER_LEFT_72_GC_PERCENT=55.000 PRIMER_RIGHT_72_GC_PERCENT=63.158 PRIMER_LEFT_72_SELF_ANY=7.00 PRIMER_RIGHT_72_SELF_ANY=4.00 PRIMER_LEFT_72_SELF_END=2.00 PRIMER_RIGHT_72_SELF_END=2.00 PRIMER_LEFT_72_END_STABILITY=6.4000 PRIMER_RIGHT_72_END_STABILITY=5.6000 PRIMER_PAIR_72_COMPL_ANY=4.00 PRIMER_PAIR_72_COMPL_END=0.00 PRIMER_PAIR_72_PRODUCT_SIZE=157 PRIMER_PAIR_73_PENALTY=4.269459 PRIMER_LEFT_73_PENALTY=3.039809 PRIMER_RIGHT_73_PENALTY=1.229650 PRIMER_LEFT_73_SEQUENCE=CCCAGGGATGGAAGTGCT PRIMER_RIGHT_73_SEQUENCE=AGGAGCAGAGGGGTCGATA PRIMER_LEFT_73=124,18 PRIMER_RIGHT_73=276,19 PRIMER_LEFT_73_TM=61.040 PRIMER_RIGHT_73_TM=59.770 PRIMER_LEFT_73_GC_PERCENT=61.111 PRIMER_RIGHT_73_GC_PERCENT=57.895 PRIMER_LEFT_73_SELF_ANY=5.00 PRIMER_RIGHT_73_SELF_ANY=4.00 PRIMER_LEFT_73_SELF_END=2.00 PRIMER_RIGHT_73_SELF_END=2.00 PRIMER_LEFT_73_END_STABILITY=7.9000 PRIMER_RIGHT_73_END_STABILITY=7.6000 PRIMER_PAIR_73_COMPL_ANY=4.00 PRIMER_PAIR_73_COMPL_END=1.00 PRIMER_PAIR_73_PRODUCT_SIZE=153 PRIMER_PAIR_74_PENALTY=4.301436 PRIMER_LEFT_74_PENALTY=3.045630 PRIMER_RIGHT_74_PENALTY=1.255806 PRIMER_LEFT_74_SEQUENCE=AGAGCCTGAGTTGGCATTC PRIMER_RIGHT_74_SEQUENCE=GCTCAGGTAGAGCTTCCGC PRIMER_LEFT_74=189,19 PRIMER_RIGHT_74=402,19 PRIMER_LEFT_74_TM=57.954 PRIMER_RIGHT_74_TM=60.256 PRIMER_LEFT_74_GC_PERCENT=52.632 PRIMER_RIGHT_74_GC_PERCENT=63.158 PRIMER_LEFT_74_SELF_ANY=3.00 PRIMER_RIGHT_74_SELF_ANY=4.00 PRIMER_LEFT_74_SELF_END=2.00 PRIMER_RIGHT_74_SELF_END=2.00 PRIMER_LEFT_74_END_STABILITY=6.9000 PRIMER_RIGHT_74_END_STABILITY=11.4000 PRIMER_PAIR_74_COMPL_ANY=6.00 PRIMER_PAIR_74_COMPL_END=0.00 PRIMER_PAIR_74_PRODUCT_SIZE=214 PRIMER_PAIR_75_PENALTY=4.323206 PRIMER_LEFT_75_PENALTY=3.045630 PRIMER_RIGHT_75_PENALTY=1.277576 PRIMER_LEFT_75_SEQUENCE=GAGAGCCTGAGTTGGCATT PRIMER_RIGHT_75_SEQUENCE=CGAGTGGTGGTTAGAGGTGA PRIMER_LEFT_75=188,19 PRIMER_RIGHT_75=366,20 PRIMER_LEFT_75_TM=57.954 PRIMER_RIGHT_75_TM=58.722 PRIMER_LEFT_75_GC_PERCENT=52.632 PRIMER_RIGHT_75_GC_PERCENT=55.000 PRIMER_LEFT_75_SELF_ANY=3.00 PRIMER_RIGHT_75_SELF_ANY=2.00 PRIMER_LEFT_75_SELF_END=1.00 PRIMER_RIGHT_75_SELF_END=1.00 PRIMER_LEFT_75_END_STABILITY=8.4000 PRIMER_RIGHT_75_END_STABILITY=7.9000 PRIMER_PAIR_75_COMPL_ANY=3.00 PRIMER_PAIR_75_COMPL_END=1.00 PRIMER_PAIR_75_PRODUCT_SIZE=179 PRIMER_PAIR_76_PENALTY=4.404428 PRIMER_LEFT_76_PENALTY=3.159514 PRIMER_RIGHT_76_PENALTY=1.244914 PRIMER_LEFT_76_SEQUENCE=GATGTGCGCAAGGAGATG PRIMER_RIGHT_76_SEQUENCE=AGGCACGTTAAGCTCAGCA PRIMER_LEFT_76=165,18 PRIMER_RIGHT_76=258,19 PRIMER_LEFT_76_TM=58.840 PRIMER_RIGHT_76_TM=59.755 PRIMER_LEFT_76_GC_PERCENT=55.556 PRIMER_RIGHT_76_GC_PERCENT=52.632 PRIMER_LEFT_76_SELF_ANY=6.00 PRIMER_RIGHT_76_SELF_ANY=5.00 PRIMER_LEFT_76_SELF_END=0.00 PRIMER_RIGHT_76_SELF_END=3.00 PRIMER_LEFT_76_END_STABILITY=6.6000 PRIMER_RIGHT_76_END_STABILITY=8.5000 PRIMER_PAIR_76_COMPL_ANY=4.00 PRIMER_PAIR_76_COMPL_END=2.00 PRIMER_PAIR_76_PRODUCT_SIZE=94 PRIMER_PAIR_77_PENALTY=4.460864 PRIMER_LEFT_77_PENALTY=3.173109 PRIMER_RIGHT_77_PENALTY=1.287755 PRIMER_LEFT_77_SEQUENCE=ATGGAAGTGCTTGGATGCG PRIMER_RIGHT_77_SEQUENCE=GTGAAGACCACGAAGATGAGG PRIMER_LEFT_77=131,19 PRIMER_RIGHT_77=878,21 PRIMER_LEFT_77_TM=62.173 PRIMER_RIGHT_77_TM=59.712 PRIMER_LEFT_77_GC_PERCENT=52.632 PRIMER_RIGHT_77_GC_PERCENT=52.381 PRIMER_LEFT_77_SELF_ANY=4.00 PRIMER_RIGHT_77_SELF_ANY=3.00 PRIMER_LEFT_77_SELF_END=2.00 PRIMER_RIGHT_77_SELF_END=0.00 PRIMER_LEFT_77_END_STABILITY=10.1000 PRIMER_RIGHT_77_END_STABILITY=8.2000 PRIMER_PAIR_77_COMPL_ANY=3.00 PRIMER_PAIR_77_COMPL_END=0.00 PRIMER_PAIR_77_PRODUCT_SIZE=748 PRIMER_PAIR_78_PENALTY=4.486063 PRIMER_LEFT_78_PENALTY=3.198308 PRIMER_RIGHT_78_PENALTY=1.287755 PRIMER_LEFT_78_SEQUENCE=TTCGTATAAATGACCTGCCTGG PRIMER_RIGHT_78_SEQUENCE=GGTGAAGACCACGAAGATGAG PRIMER_LEFT_78=205,22 PRIMER_RIGHT_78=879,21 PRIMER_LEFT_78_TM=61.198 PRIMER_RIGHT_78_TM=59.712 PRIMER_LEFT_78_GC_PERCENT=45.455 PRIMER_RIGHT_78_GC_PERCENT=52.381 PRIMER_LEFT_78_SELF_ANY=4.00 PRIMER_RIGHT_78_SELF_ANY=3.00 PRIMER_LEFT_78_SELF_END=3.00 PRIMER_RIGHT_78_SELF_END=0.00 PRIMER_LEFT_78_END_STABILITY=9.7000 PRIMER_RIGHT_78_END_STABILITY=6.6000 PRIMER_PAIR_78_COMPL_ANY=5.00 PRIMER_PAIR_78_COMPL_END=3.00 PRIMER_PAIR_78_PRODUCT_SIZE=675 PRIMER_PAIR_79_PENALTY=4.490383 PRIMER_LEFT_79_PENALTY=3.200839 PRIMER_RIGHT_79_PENALTY=1.289544 PRIMER_LEFT_79_SEQUENCE=AATGACCTGCCTGGCTCC PRIMER_RIGHT_79_SEQUENCE=CAGCTGGACCTTGAGCTTG PRIMER_LEFT_79=213,18 PRIMER_RIGHT_79=966,19 PRIMER_LEFT_79_TM=61.201 PRIMER_RIGHT_79_TM=59.710 PRIMER_LEFT_79_GC_PERCENT=61.111 PRIMER_RIGHT_79_GC_PERCENT=57.895 PRIMER_LEFT_79_SELF_ANY=5.00 PRIMER_RIGHT_79_SELF_ANY=6.00 PRIMER_LEFT_79_SELF_END=2.00 PRIMER_RIGHT_79_SELF_END=2.00 PRIMER_LEFT_79_END_STABILITY=9.4000 PRIMER_RIGHT_79_END_STABILITY=8.5000 PRIMER_PAIR_79_COMPL_ANY=4.00 PRIMER_PAIR_79_COMPL_END=2.00 PRIMER_PAIR_79_PRODUCT_SIZE=754 PRIMER_PAIR_80_PENALTY=4.556874 PRIMER_LEFT_80_PENALTY=3.199824 PRIMER_RIGHT_80_PENALTY=1.357049 PRIMER_LEFT_80_SEQUENCE=GTCCCAGGGATGGAAGTG PRIMER_RIGHT_80_SEQUENCE=GGTGAAGACCACGAAGATGA PRIMER_LEFT_80=122,18 PRIMER_RIGHT_80=879,20 PRIMER_LEFT_80_TM=58.800 PRIMER_RIGHT_80_TM=58.643 PRIMER_LEFT_80_GC_PERCENT=61.111 PRIMER_RIGHT_80_GC_PERCENT=50.000 PRIMER_LEFT_80_SELF_ANY=7.00 PRIMER_RIGHT_80_SELF_ANY=3.00 PRIMER_LEFT_80_SELF_END=0.00 PRIMER_RIGHT_80_SELF_END=1.00 PRIMER_LEFT_80_END_STABILITY=6.7000 PRIMER_RIGHT_80_END_STABILITY=6.6000 PRIMER_PAIR_80_COMPL_ANY=3.00 PRIMER_PAIR_80_COMPL_END=0.00 PRIMER_PAIR_80_PRODUCT_SIZE=758 PRIMER_PAIR_81_PENALTY=4.561659 PRIMER_LEFT_81_PENALTY=3.204610 PRIMER_RIGHT_81_PENALTY=1.357049 PRIMER_LEFT_81_SEQUENCE=TTCGTATAAATGACCTGCCTG PRIMER_RIGHT_81_SEQUENCE=GATGGTGAAGACCACGAAGA PRIMER_LEFT_81=205,21 PRIMER_RIGHT_81=882,20 PRIMER_LEFT_81_TM=57.795 PRIMER_RIGHT_81_TM=58.643 PRIMER_LEFT_81_GC_PERCENT=42.857 PRIMER_RIGHT_81_GC_PERCENT=50.000 PRIMER_LEFT_81_SELF_ANY=4.00 PRIMER_RIGHT_81_SELF_ANY=4.00 PRIMER_LEFT_81_SELF_END=1.00 PRIMER_RIGHT_81_SELF_END=0.00 PRIMER_LEFT_81_END_STABILITY=9.7000 PRIMER_RIGHT_81_END_STABILITY=6.7000 PRIMER_PAIR_81_COMPL_ANY=5.00 PRIMER_PAIR_81_COMPL_END=2.00 PRIMER_PAIR_81_PRODUCT_SIZE=678 PRIMER_PAIR_82_PENALTY=4.573274 PRIMER_LEFT_82_PENALTY=3.204610 PRIMER_RIGHT_82_PENALTY=1.368664 PRIMER_LEFT_82_SEQUENCE=CCTGAGTTGGCATTCGTATAA PRIMER_RIGHT_82_SEQUENCE=CGAGTGGTGGTTAGAGGTGAC PRIMER_LEFT_82=193,21 PRIMER_RIGHT_82=366,21 PRIMER_LEFT_82_TM=57.795 PRIMER_RIGHT_82_TM=59.631 PRIMER_LEFT_82_GC_PERCENT=42.857 PRIMER_RIGHT_82_GC_PERCENT=57.143 PRIMER_LEFT_82_SELF_ANY=4.00 PRIMER_RIGHT_82_SELF_ANY=3.00 PRIMER_LEFT_82_SELF_END=2.00 PRIMER_RIGHT_82_SELF_END=3.00 PRIMER_LEFT_82_END_STABILITY=5.2000 PRIMER_RIGHT_82_END_STABILITY=6.1000 PRIMER_PAIR_82_COMPL_ANY=4.00 PRIMER_PAIR_82_COMPL_END=1.00 PRIMER_PAIR_82_PRODUCT_SIZE=174 PRIMER_PAIR_83_PENALTY=4.592877 PRIMER_LEFT_83_PENALTY=3.227824 PRIMER_RIGHT_83_PENALTY=1.365052 PRIMER_LEFT_83_SEQUENCE=AACGTCCCAGGGATGGAAG PRIMER_RIGHT_83_SEQUENCE=ATGCCAACTCAGGCTCTCC PRIMER_LEFT_83=119,19 PRIMER_RIGHT_83=205,19 PRIMER_LEFT_83_TM=62.228 PRIMER_RIGHT_83_TM=60.365 PRIMER_LEFT_83_GC_PERCENT=57.895 PRIMER_RIGHT_83_GC_PERCENT=57.895 PRIMER_LEFT_83_SELF_ANY=7.00 PRIMER_RIGHT_83_SELF_ANY=3.00 PRIMER_LEFT_83_SELF_END=0.00 PRIMER_RIGHT_83_SELF_END=0.00 PRIMER_LEFT_83_END_STABILITY=8.2000 PRIMER_RIGHT_83_END_STABILITY=7.9000 PRIMER_PAIR_83_COMPL_ANY=4.00 PRIMER_PAIR_83_COMPL_END=3.00 PRIMER_PAIR_83_PRODUCT_SIZE=87 PRIMER_PAIR_84_PENALTY=4.627137 PRIMER_LEFT_84_PENALTY=3.251078 PRIMER_RIGHT_84_PENALTY=1.376058 PRIMER_LEFT_84_SEQUENCE=AAATGACCTGCCTGGCTC PRIMER_RIGHT_84_SEQUENCE=GGACTCGCTGATGGAGTTCA PRIMER_LEFT_84=212,18 PRIMER_RIGHT_84=645,20 PRIMER_LEFT_84_TM=58.749 PRIMER_RIGHT_84_TM=61.376 PRIMER_LEFT_84_GC_PERCENT=55.556 PRIMER_RIGHT_84_GC_PERCENT=55.000 PRIMER_LEFT_84_SELF_ANY=5.00 PRIMER_RIGHT_84_SELF_ANY=5.00 PRIMER_LEFT_84_SELF_END=3.00 PRIMER_RIGHT_84_SELF_END=3.00 PRIMER_LEFT_84_END_STABILITY=9.4000 PRIMER_RIGHT_84_END_STABILITY=6.7000 PRIMER_PAIR_84_COMPL_ANY=4.00 PRIMER_PAIR_84_COMPL_END=2.00 PRIMER_PAIR_84_PRODUCT_SIZE=434 PRIMER_PAIR_85_PENALTY=4.665035 PRIMER_LEFT_85_PENALTY=3.284613 PRIMER_RIGHT_85_PENALTY=1.380422 PRIMER_LEFT_85_SEQUENCE=ATGGGATGGAGAGCCTGAGTT PRIMER_RIGHT_85_SEQUENCE=GATGCAGGTGCTGATGAGG PRIMER_LEFT_85=180,21 PRIMER_RIGHT_85=588,19 PRIMER_LEFT_85_TM=62.285 PRIMER_RIGHT_85_TM=60.380 PRIMER_LEFT_85_GC_PERCENT=52.381 PRIMER_RIGHT_85_GC_PERCENT=57.895 PRIMER_LEFT_85_SELF_ANY=3.00 PRIMER_RIGHT_85_SELF_ANY=4.00 PRIMER_LEFT_85_SELF_END=0.00 PRIMER_RIGHT_85_SELF_END=2.00 PRIMER_LEFT_85_END_STABILITY=6.4000 PRIMER_RIGHT_85_END_STABILITY=8.2000 PRIMER_PAIR_85_COMPL_ANY=4.00 PRIMER_PAIR_85_COMPL_END=1.00 PRIMER_PAIR_85_PRODUCT_SIZE=409 PRIMER_PAIR_86_PENALTY=4.674115 PRIMER_LEFT_86_PENALTY=3.293693 PRIMER_RIGHT_86_PENALTY=1.380422 PRIMER_LEFT_86_SEQUENCE=AACGTCCCAGGGATGGAA PRIMER_RIGHT_86_SEQUENCE=AGGATGCAGGTGCTGATGA PRIMER_LEFT_86=119,18 PRIMER_RIGHT_86=590,19 PRIMER_LEFT_86_TM=61.294 PRIMER_RIGHT_86_TM=60.380 PRIMER_LEFT_86_GC_PERCENT=55.556 PRIMER_RIGHT_86_GC_PERCENT=52.632 PRIMER_LEFT_86_SELF_ANY=7.00 PRIMER_RIGHT_86_SELF_ANY=4.00 PRIMER_LEFT_86_SELF_END=2.00 PRIMER_RIGHT_86_SELF_END=2.00 PRIMER_LEFT_86_END_STABILITY=8.5000 PRIMER_RIGHT_86_END_STABILITY=6.6000 PRIMER_PAIR_86_COMPL_ANY=3.00 PRIMER_PAIR_86_COMPL_END=0.00 PRIMER_PAIR_86_PRODUCT_SIZE=472 PRIMER_PAIR_87_PENALTY=4.717135 PRIMER_LEFT_87_PENALTY=3.331781 PRIMER_RIGHT_87_PENALTY=1.385354 PRIMER_LEFT_87_SEQUENCE=CCCACCATGAGTGCTGAG PRIMER_RIGHT_87_SEQUENCE=TGGACCTTGAGCTTGTGGA PRIMER_LEFT_87=229,18 PRIMER_RIGHT_87=962,19 PRIMER_LEFT_87_TM=58.668 PRIMER_RIGHT_87_TM=60.385 PRIMER_LEFT_87_GC_PERCENT=61.111 PRIMER_RIGHT_87_GC_PERCENT=52.632 PRIMER_LEFT_87_SELF_ANY=6.00 PRIMER_RIGHT_87_SELF_ANY=4.00 PRIMER_LEFT_87_SELF_END=3.00 PRIMER_RIGHT_87_SELF_END=0.00 PRIMER_LEFT_87_END_STABILITY=6.7000 PRIMER_RIGHT_87_END_STABILITY=7.9000 PRIMER_PAIR_87_COMPL_ANY=4.00 PRIMER_PAIR_87_COMPL_END=0.00 PRIMER_PAIR_87_PRODUCT_SIZE=734 PRIMER_PAIR_88_PENALTY=4.721721 PRIMER_LEFT_88_PENALTY=3.331781 PRIMER_RIGHT_88_PENALTY=1.389940 PRIMER_LEFT_88_SEQUENCE=CTCCCACCATGAGTGCTG PRIMER_RIGHT_88_SEQUENCE=TGGACCTTGAGCTTGTGGAG PRIMER_LEFT_88=227,18 PRIMER_RIGHT_88=962,20 PRIMER_LEFT_88_TM=58.668 PRIMER_RIGHT_88_TM=61.390 PRIMER_LEFT_88_GC_PERCENT=61.111 PRIMER_RIGHT_88_GC_PERCENT=55.000 PRIMER_LEFT_88_SELF_ANY=6.00 PRIMER_RIGHT_88_SELF_ANY=4.00 PRIMER_LEFT_88_SELF_END=3.00 PRIMER_RIGHT_88_SELF_END=0.00 PRIMER_LEFT_88_END_STABILITY=8.5000 PRIMER_RIGHT_88_END_STABILITY=8.2000 PRIMER_PAIR_88_COMPL_ANY=4.00 PRIMER_PAIR_88_COMPL_END=1.00 PRIMER_PAIR_88_PRODUCT_SIZE=736 PRIMER_PAIR_89_PENALTY=4.723310 PRIMER_LEFT_89_PENALTY=3.333370 PRIMER_RIGHT_89_PENALTY=1.389940 PRIMER_LEFT_89_SEQUENCE=GCCTGAGTTGGCATTCGTATAA PRIMER_RIGHT_89_SEQUENCE=CTGGACCTTGAGCTTGTGGA PRIMER_LEFT_89=192,22 PRIMER_RIGHT_89=963,20 PRIMER_LEFT_89_TM=61.333 PRIMER_RIGHT_89_TM=61.390 PRIMER_LEFT_89_GC_PERCENT=45.455 PRIMER_RIGHT_89_GC_PERCENT=55.000 PRIMER_LEFT_89_SELF_ANY=4.00 PRIMER_RIGHT_89_SELF_ANY=4.00 PRIMER_LEFT_89_SELF_END=2.00 PRIMER_RIGHT_89_SELF_END=0.00 PRIMER_LEFT_89_END_STABILITY=5.2000 PRIMER_RIGHT_89_END_STABILITY=7.9000 PRIMER_PAIR_89_COMPL_ANY=2.00 PRIMER_PAIR_89_COMPL_END=1.00 PRIMER_PAIR_89_PRODUCT_SIZE=772 PRIMER_PAIR_90_PENALTY=4.810150 PRIMER_LEFT_90_PENALTY=3.413779 PRIMER_RIGHT_90_PENALTY=1.396371 PRIMER_LEFT_90_SEQUENCE=AGGGATGGAAGTGCTTGG PRIMER_RIGHT_90_SEQUENCE=ATGCAGGTGCTGATGAGGAG PRIMER_LEFT_90=127,18 PRIMER_RIGHT_90=587,20 PRIMER_LEFT_90_TM=58.586 PRIMER_RIGHT_90_TM=61.396 PRIMER_LEFT_90_GC_PERCENT=55.556 PRIMER_RIGHT_90_GC_PERCENT=55.000 PRIMER_LEFT_90_SELF_ANY=4.00 PRIMER_RIGHT_90_SELF_ANY=4.00 PRIMER_LEFT_90_SELF_END=0.00 PRIMER_RIGHT_90_SELF_END=2.00 PRIMER_LEFT_90_END_STABILITY=8.5000 PRIMER_RIGHT_90_END_STABILITY=7.9000 PRIMER_PAIR_90_COMPL_ANY=3.00 PRIMER_PAIR_90_COMPL_END=0.00 PRIMER_PAIR_90_PRODUCT_SIZE=461 PRIMER_PAIR_91_PENALTY=4.913881 PRIMER_LEFT_91_PENALTY=3.514925 PRIMER_RIGHT_91_PENALTY=1.398956 PRIMER_LEFT_91_SEQUENCE=AAATGACCTGCCTGGCTCC PRIMER_RIGHT_91_SEQUENCE=CGGTAGAAGTGGATGGTGAAG PRIMER_LEFT_91=212,19 PRIMER_RIGHT_91=893,21 PRIMER_LEFT_91_TM=62.515 PRIMER_RIGHT_91_TM=59.601 PRIMER_LEFT_91_GC_PERCENT=57.895 PRIMER_RIGHT_91_GC_PERCENT=52.381 PRIMER_LEFT_91_SELF_ANY=5.00 PRIMER_RIGHT_91_SELF_ANY=2.00 PRIMER_LEFT_91_SELF_END=2.00 PRIMER_RIGHT_91_SELF_END=0.00 PRIMER_LEFT_91_END_STABILITY=9.4000 PRIMER_RIGHT_91_END_STABILITY=7.0000 PRIMER_PAIR_91_COMPL_ANY=3.00 PRIMER_PAIR_91_COMPL_END=2.00 PRIMER_PAIR_91_PRODUCT_SIZE=682 PRIMER_PAIR_92_PENALTY=4.959245 PRIMER_LEFT_92_PENALTY=3.548727 PRIMER_RIGHT_92_PENALTY=1.410518 PRIMER_LEFT_92_SEQUENCE=CACCATGAGTGCTGAGCTT PRIMER_RIGHT_92_SEQUENCE=AGAGGTGACCAGTTCCAGGT PRIMER_LEFT_92=231,19 PRIMER_RIGHT_92=354,20 PRIMER_LEFT_92_TM=57.451 PRIMER_RIGHT_92_TM=58.589 PRIMER_LEFT_92_GC_PERCENT=52.632 PRIMER_RIGHT_92_GC_PERCENT=55.000 PRIMER_LEFT_92_SELF_ANY=6.00 PRIMER_RIGHT_92_SELF_ANY=5.00 PRIMER_LEFT_92_SELF_END=2.00 PRIMER_RIGHT_92_SELF_END=3.00 PRIMER_LEFT_92_END_STABILITY=8.2000 PRIMER_RIGHT_92_END_STABILITY=7.9000 PRIMER_PAIR_92_COMPL_ANY=4.00 PRIMER_PAIR_92_COMPL_END=2.00 PRIMER_PAIR_92_PRODUCT_SIZE=124 PRIMER_PAIR_93_PENALTY=4.979019 PRIMER_LEFT_93_PENALTY=3.565929 PRIMER_RIGHT_93_PENALTY=1.413090 PRIMER_LEFT_93_SEQUENCE=ATAAATGACCTGCCTGGCTCC PRIMER_RIGHT_93_SEQUENCE=CTGGTACTGCGTCTCCAGC PRIMER_LEFT_93=210,21 PRIMER_RIGHT_93=498,19 PRIMER_LEFT_93_TM=62.566 PRIMER_RIGHT_93_TM=59.587 PRIMER_LEFT_93_GC_PERCENT=52.381 PRIMER_RIGHT_93_GC_PERCENT=63.158 PRIMER_LEFT_93_SELF_ANY=5.00 PRIMER_RIGHT_93_SELF_ANY=4.00 PRIMER_LEFT_93_SELF_END=2.00 PRIMER_RIGHT_93_SELF_END=2.00 PRIMER_LEFT_93_END_STABILITY=9.4000 PRIMER_RIGHT_93_END_STABILITY=9.7000 PRIMER_PAIR_93_COMPL_ANY=4.00 PRIMER_PAIR_93_COMPL_END=1.00 PRIMER_PAIR_93_PRODUCT_SIZE=289 PRIMER_PAIR_94_PENALTY=5.004099 PRIMER_LEFT_94_PENALTY=3.588984 PRIMER_RIGHT_94_PENALTY=1.415114 PRIMER_LEFT_94_SEQUENCE=GAGCCTGAGTTGGCATTCGTA PRIMER_RIGHT_94_SEQUENCE=TGGAGTTCAGGTTGTGGATGT PRIMER_LEFT_94=190,21 PRIMER_RIGHT_94=634,21 PRIMER_LEFT_94_TM=62.589 PRIMER_RIGHT_94_TM=60.415 PRIMER_LEFT_94_GC_PERCENT=52.381 PRIMER_RIGHT_94_GC_PERCENT=47.619 PRIMER_LEFT_94_SELF_ANY=3.00 PRIMER_RIGHT_94_SELF_ANY=3.00 PRIMER_LEFT_94_SELF_END=2.00 PRIMER_RIGHT_94_SELF_END=0.00 PRIMER_LEFT_94_END_STABILITY=7.4000 PRIMER_RIGHT_94_END_STABILITY=6.3000 PRIMER_PAIR_94_COMPL_ANY=5.00 PRIMER_PAIR_94_COMPL_END=1.00 PRIMER_PAIR_94_PRODUCT_SIZE=445 PRIMER_PAIR_95_PENALTY=5.156071 PRIMER_LEFT_95_PENALTY=3.724668 PRIMER_RIGHT_95_PENALTY=1.431404 PRIMER_LEFT_95_SEQUENCE=CATTCGTATAAATGACCTGCCTG PRIMER_RIGHT_95_SEQUENCE=CGGTAGAAGTGGATGGTGAA PRIMER_LEFT_95=203,23 PRIMER_RIGHT_95=893,20 PRIMER_LEFT_95_TM=60.725 PRIMER_RIGHT_95_TM=58.569 PRIMER_LEFT_95_GC_PERCENT=43.478 PRIMER_RIGHT_95_GC_PERCENT=50.000 PRIMER_LEFT_95_SELF_ANY=6.00 PRIMER_RIGHT_95_SELF_ANY=2.00 PRIMER_LEFT_95_SELF_END=1.00 PRIMER_RIGHT_95_SELF_END=0.00 PRIMER_LEFT_95_END_STABILITY=9.7000 PRIMER_RIGHT_95_END_STABILITY=6.7000 PRIMER_PAIR_95_COMPL_ANY=3.00 PRIMER_PAIR_95_COMPL_END=0.00 PRIMER_PAIR_95_PRODUCT_SIZE=691 PRIMER_PAIR_96_PENALTY=5.174301 PRIMER_LEFT_96_PENALTY=3.785894 PRIMER_RIGHT_96_PENALTY=1.388407 PRIMER_LEFT_96_SEQUENCE=GATGTGCGCAAGGAGATGG PRIMER_RIGHT_96_SEQUENCE=GGCACGTTAAGCTCAGCAC PRIMER_LEFT_96=165,19 PRIMER_RIGHT_96=257,19 PRIMER_LEFT_96_TM=62.786 PRIMER_RIGHT_96_TM=59.612 PRIMER_LEFT_96_GC_PERCENT=57.895 PRIMER_RIGHT_96_GC_PERCENT=57.895 PRIMER_LEFT_96_SELF_ANY=6.00 PRIMER_RIGHT_96_SELF_ANY=5.00 PRIMER_LEFT_96_SELF_END=0.00 PRIMER_RIGHT_96_SELF_END=1.00 PRIMER_LEFT_96_END_STABILITY=8.1000 PRIMER_RIGHT_96_END_STABILITY=7.9000 PRIMER_PAIR_96_COMPL_ANY=4.00 PRIMER_PAIR_96_COMPL_END=1.00 PRIMER_PAIR_96_PRODUCT_SIZE=93 PRIMER_PAIR_97_PENALTY=5.207404 PRIMER_LEFT_97_PENALTY=4.813185 PRIMER_RIGHT_97_PENALTY=0.394220 PRIMER_LEFT_97_SEQUENCE=GGGAAGGAACGTCCCAGG PRIMER_RIGHT_97_SEQUENCE=CTCTCCATCCCATCTCCTTG PRIMER_LEFT_97=112,18 PRIMER_RIGHT_97=192,20 PRIMER_LEFT_97_TM=62.813 PRIMER_RIGHT_97_TM=59.606 PRIMER_LEFT_97_GC_PERCENT=66.667 PRIMER_RIGHT_97_GC_PERCENT=55.000 PRIMER_LEFT_97_SELF_ANY=6.00 PRIMER_RIGHT_97_SELF_ANY=2.00 PRIMER_LEFT_97_SELF_END=3.00 PRIMER_RIGHT_97_SELF_END=0.00 PRIMER_LEFT_97_END_STABILITY=9.7000 PRIMER_RIGHT_97_END_STABILITY=8.5000 PRIMER_PAIR_97_COMPL_ANY=6.00 PRIMER_PAIR_97_COMPL_END=3.00 PRIMER_PAIR_97_PRODUCT_SIZE=81 PRIMER_PAIR_98_PENALTY=5.214462 PRIMER_LEFT_98_PENALTY=3.782399 PRIMER_RIGHT_98_PENALTY=1.432063 PRIMER_LEFT_98_SEQUENCE=ATAAATGACCTGCCTGGCT PRIMER_RIGHT_98_SEQUENCE=AGGTTGTGGATGTTGCTCAC PRIMER_LEFT_98=210,19 PRIMER_RIGHT_98=626,20 PRIMER_LEFT_98_TM=57.218 PRIMER_RIGHT_98_TM=58.568 PRIMER_LEFT_98_GC_PERCENT=47.368 PRIMER_RIGHT_98_GC_PERCENT=50.000 PRIMER_LEFT_98_SELF_ANY=5.00 PRIMER_RIGHT_98_SELF_ANY=3.00 PRIMER_LEFT_98_SELF_END=3.00 PRIMER_RIGHT_98_SELF_END=0.00 PRIMER_LEFT_98_END_STABILITY=9.7000 PRIMER_RIGHT_98_END_STABILITY=6.4000 PRIMER_PAIR_98_COMPL_ANY=4.00 PRIMER_PAIR_98_COMPL_END=0.00 PRIMER_PAIR_98_PRODUCT_SIZE=417 PRIMER_PAIR_99_PENALTY=5.221100 PRIMER_LEFT_99_PENALTY=3.785894 PRIMER_RIGHT_99_PENALTY=1.435206 PRIMER_LEFT_99_SEQUENCE=GGATGTGCGCAAGGAGATG PRIMER_RIGHT_99_SEQUENCE=TGGTGGTTAGAGGTGACCAG PRIMER_LEFT_99=164,19 PRIMER_RIGHT_99=362,20 PRIMER_LEFT_99_TM=62.786 PRIMER_RIGHT_99_TM=58.565 PRIMER_LEFT_99_GC_PERCENT=57.895 PRIMER_RIGHT_99_GC_PERCENT=55.000 PRIMER_LEFT_99_SELF_ANY=6.00 PRIMER_RIGHT_99_SELF_ANY=5.00 PRIMER_LEFT_99_SELF_END=0.00 PRIMER_RIGHT_99_SELF_END=2.00 PRIMER_LEFT_99_END_STABILITY=6.6000 PRIMER_RIGHT_99_END_STABILITY=7.9000 PRIMER_PAIR_99_COMPL_ANY=2.00 PRIMER_PAIR_99_COMPL_END=1.00 PRIMER_PAIR_99_PRODUCT_SIZE=199 = SEQUENCE_ID=ok_regions_test3 SEQUENCE_TEMPLATE=GGATCAGCTGACTCGCCTGGCTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGCCGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTGTGTGGCAGCGCCATGTCCCAAAGTTTACTCAAGACTACCCAGGAACCTCTTGCCCGAGATCCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGATGAGCTGCTTCAGAAAGAGCAAAACTATTCAGATGACGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCATCTTTGACCGAAACGAAAACCACCGTGGAGCTCCTTCCCGTGAATGGAGAGTTCAGCCTGGACGATCTCCAGCCGTGGCATTCTTTTGGGGCTGACTCTGTGCCAGCCAACACAGAAAACGAAGTTGAGCCTGTTGATGCCCGCCCTGCTGCCGACCGAGGACTGACCACTCGACCAGGTTCTGGGTTGACAAATATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGACTCAGGATATGAAGTTCATCATCAAAAATTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGTGCAATCATTGGACTCATGGTGGGCGGTGTTGTCATAGCGACAGTGATCGTCATCACCTTGGTGATGCTGAAGAAGAAACAGTACACATCCATTCATCATGGTGTGGTGGAGGTTGACGCCGCTGTCACCCCAGAGGAGCGCCACCTGTCCAAGATGCAGCAGAACGGCTACGAAAATCCAACCTACAAGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCTCTGAAGTTGGACAGCAAAACCATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGGGAAGAAACAAACCCGTTTTATGATTTACTCATTATCGCCTTTTGACAGCTGTGCTGTAACACAAGTAGATGCCTGAACTTGAATTAATCCACACATCAGTAATGTATTCTATCTCTCTTTACATTTTGGTCTCTATACTACATTATTAATGGGTTTTGTGTACTGTAAAGAATTTAGCTGTATCAAACTAGTGCATGAATAGATTCTCTCCTGATTATTTATCACATAGCCCCTTAGCCAGTTGTATATTATTCTTGTGGTTTGTGACCCAATTAAGTCCTACTTTACATATGCTTTAAGAATCGATGGGGGATGCTTCATGTGAACGTGGGAGTTCAGCTGCTTCTCTTGCCTAAGTATTCCTTTCCTGATCACTATGCATTTTAAAGTTAAACATTTTTAAGTATTTCAGATGCTTTAGAGAGATTTTTTTTCCATGACTGCATTTTACTGTACAGATTGCTGCTTCTGCTATATTTGTGATATAGGAATTAAGAGGATACACACGTTTGTTTCTTCGTGCCTGTTTTATGTGCACACATTAGGCATTGAGACTTCAAGCTTTTCTTTTTTTGTCCACGTATCTTTGGGTCTTTGATAAAGAAAAGAATCCCTGTTCATTGTAAGCACTTTTACGGGGCGGGTGGGGAGGGGTGCTCTGCTGGTCTTCAATTACCAAGAATTCTCCAAAACAATTTTCTGCAGGATGATTGTACAGAATCATTGCTTATGACATGATCGCTTTCTACACTGTATTACATAAATAAATTAAATAAAATAACCCCGGGCAAGACTTTTCTTTGAAGGATGACTACAGACATTAAATAATCGAAGTAATTTTGGGTGGGGAGAAGAGGCAGATTCAATTTTCTTTAACCAGTCTGAAGTTTCATTTATGATACAAAAGAAGATGAAAATGGAAGTGGCAATATAAGGGGATGAGGAAGGCATGCCTGGACAAACCCTTCTTTTAAGATGTGTCTTCAATTTGTATAAAATGGTGTTTTCATGTAAATAAATACATTCTTGGAGGAGCAAAAAAAAAAAAAAAA PRIMER_NUM_RETURN=900 PRIMER_PRODUCT_SIZE_RANGE=75-100 150-250 300-400 500-600 650-800 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=237,452,,;,,237,452;238,216,200,1500;0,1000,238,216 PRIMER_MIN_THREE_PRIME_DISTANCE=0 PRIMER_LEFT_EXPLAIN=considered 35740, GC content failed 759, low tm 702, high tm 4088, high any compl 30, high end compl 294, not in any ok left region 29065, ok 802 PRIMER_RIGHT_EXPLAIN=considered 35740, GC content failed 17, low tm 2090, high tm 7100, high any compl 36, high end compl 297, not in any ok right region 23605, ok 2595 PRIMER_PAIR_EXPLAIN=considered 1353277, unacceptable product size 1352154, high any compl 1, high end compl 105, primer in pair overlaps a primer in a better pair 6734138, ok 1017 PRIMER_LEFT_NUM_RETURNED=802 PRIMER_RIGHT_NUM_RETURNED=802 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=802 PRIMER_PAIR_0_PENALTY=0.033124 PRIMER_LEFT_0_PENALTY=0.024663 PRIMER_RIGHT_0_PENALTY=0.008461 PRIMER_LEFT_0_SEQUENCE=CTCGTTCCTGACAAGTGCAA PRIMER_RIGHT_0_SEQUENCE=TCACTGCATGTCTCTTTGGC PRIMER_LEFT_0=581,20 PRIMER_RIGHT_0=678,20 PRIMER_LEFT_0_TM=60.025 PRIMER_RIGHT_0_TM=59.992 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=10.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=98 PRIMER_PAIR_1_PENALTY=0.111900 PRIMER_LEFT_1_PENALTY=0.008461 PRIMER_RIGHT_1_PENALTY=0.103439 PRIMER_LEFT_1_SEQUENCE=GCCAAAGAGACATGCAGTGA PRIMER_RIGHT_1_SEQUENCE=CTACCCCTCGGAACTTGTCA PRIMER_LEFT_1=659,20 PRIMER_RIGHT_1=746,20 PRIMER_LEFT_1_TM=59.992 PRIMER_RIGHT_1_TM=60.103 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=88 PRIMER_PAIR_2_PENALTY=0.197287 PRIMER_LEFT_2_PENALTY=0.172624 PRIMER_RIGHT_2_PENALTY=0.024663 PRIMER_LEFT_2_SEQUENCE=CATCCCCACTTTGTGATTCC PRIMER_RIGHT_2_SEQUENCE=TTGCACTTGTCAGGAACGAG PRIMER_LEFT_2=521,20 PRIMER_RIGHT_2=600,20 PRIMER_LEFT_2_TM=60.173 PRIMER_RIGHT_2_TM=60.025 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=8.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=0.315491 PRIMER_LEFT_3_PENALTY=0.112124 PRIMER_RIGHT_3_PENALTY=0.203367 PRIMER_LEFT_3_SEQUENCE=CAGATTGCCATGTTCTGTGG PRIMER_RIGHT_3_SEQUENCE=ATCAATGCAGGTTTTGGTCC PRIMER_LEFT_3=296,20 PRIMER_RIGHT_3=391,20 PRIMER_LEFT_3_TM=60.112 PRIMER_RIGHT_3_TM=59.797 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=9.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=96 PRIMER_PAIR_4_PENALTY=0.335297 PRIMER_LEFT_4_PENALTY=0.172624 PRIMER_RIGHT_4_PENALTY=0.162673 PRIMER_LEFT_4_SEQUENCE=CCATCCCCACTTTGTGATTC PRIMER_RIGHT_4_SEQUENCE=CTTGTCAGGAACGAGAAGGG PRIMER_LEFT_4=520,20 PRIMER_RIGHT_4=595,20 PRIMER_LEFT_4_TM=60.173 PRIMER_RIGHT_4_TM=59.837 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 PRIMER_PAIR_5_PENALTY=0.459489 PRIMER_LEFT_5_PENALTY=0.398877 PRIMER_RIGHT_5_PENALTY=0.060612 PRIMER_LEFT_5_SEQUENCE=TCCCTACCGCTGCTTAGTTG PRIMER_RIGHT_5_SEQUENCE=GTTTCGCAAACATCCATCCT PRIMER_LEFT_5=538,20 PRIMER_RIGHT_5=636,20 PRIMER_LEFT_5_TM=60.399 PRIMER_RIGHT_5_TM=59.939 PRIMER_LEFT_5_GC_PERCENT=55.000 PRIMER_RIGHT_5_GC_PERCENT=45.000 PRIMER_LEFT_5_SELF_ANY=3.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_END_STABILITY=6.7000 PRIMER_RIGHT_5_END_STABILITY=7.8000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=99 PRIMER_PAIR_6_PENALTY=0.463993 PRIMER_LEFT_6_PENALTY=0.220845 PRIMER_RIGHT_6_PENALTY=0.243148 PRIMER_LEFT_6_SEQUENCE=ATGTCCAGAATGGGAAGTGG PRIMER_RIGHT_6_SEQUENCE=GCAATACTGCAGGATGCCTT PRIMER_LEFT_6=336,20 PRIMER_RIGHT_6=418,20 PRIMER_LEFT_6_TM=59.779 PRIMER_RIGHT_6_TM=60.243 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_LEFT_6_SELF_ANY=3.00 PRIMER_RIGHT_6_SELF_ANY=8.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_END_STABILITY=7.9000 PRIMER_RIGHT_6_END_STABILITY=9.7000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PAIR_6_PRODUCT_SIZE=83 PRIMER_PAIR_7_PENALTY=0.513556 PRIMER_LEFT_7_PENALTY=0.452945 PRIMER_RIGHT_7_PENALTY=0.060612 PRIMER_LEFT_7_SEQUENCE=CCGCTGCTTAGTTGGTGAGT PRIMER_RIGHT_7_SEQUENCE=AGTTTCGCAAACATCCATCC PRIMER_LEFT_7=544,20 PRIMER_RIGHT_7=637,20 PRIMER_LEFT_7_TM=60.453 PRIMER_RIGHT_7_TM=59.939 PRIMER_LEFT_7_GC_PERCENT=55.000 PRIMER_RIGHT_7_GC_PERCENT=45.000 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=1.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=6.4000 PRIMER_RIGHT_7_END_STABILITY=8.1000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PAIR_7_PRODUCT_SIZE=94 PRIMER_PAIR_8_PENALTY=0.523640 PRIMER_LEFT_8_PENALTY=0.237616 PRIMER_RIGHT_8_PENALTY=0.286024 PRIMER_LEFT_8_SEQUENCE=TGGAGGTACCCACTGATGGT PRIMER_RIGHT_8_SEQUENCE=ATGTGCATGTTCAGTCTGCC PRIMER_LEFT_8=252,20 PRIMER_RIGHT_8=333,20 PRIMER_LEFT_8_TM=60.238 PRIMER_RIGHT_8_TM=59.714 PRIMER_LEFT_8_GC_PERCENT=55.000 PRIMER_RIGHT_8_GC_PERCENT=50.000 PRIMER_LEFT_8_SELF_ANY=6.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=1.00 PRIMER_LEFT_8_END_STABILITY=7.8000 PRIMER_RIGHT_8_END_STABILITY=9.7000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PAIR_8_PRODUCT_SIZE=82 PRIMER_PAIR_9_PENALTY=0.532592 PRIMER_LEFT_9_PENALTY=0.452945 PRIMER_RIGHT_9_PENALTY=0.079648 PRIMER_LEFT_9_SEQUENCE=ACCGCTGCTTAGTTGGTGAG PRIMER_RIGHT_9_SEQUENCE=CATCCATCCTCTCCTGGTGT PRIMER_LEFT_9=543,20 PRIMER_RIGHT_9=626,20 PRIMER_LEFT_9_TM=60.453 PRIMER_RIGHT_9_TM=59.920 PRIMER_LEFT_9_GC_PERCENT=55.000 PRIMER_RIGHT_9_GC_PERCENT=55.000 PRIMER_LEFT_9_SELF_ANY=3.00 PRIMER_RIGHT_9_SELF_ANY=3.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=1.00 PRIMER_LEFT_9_END_STABILITY=6.4000 PRIMER_RIGHT_9_END_STABILITY=7.6000 PRIMER_PAIR_9_COMPL_ANY=4.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PAIR_9_PRODUCT_SIZE=84 PRIMER_PAIR_10_PENALTY=0.580565 PRIMER_LEFT_10_PENALTY=0.407941 PRIMER_RIGHT_10_PENALTY=0.172624 PRIMER_LEFT_10_SEQUENCE=GGTAGAAGCCAACCAACCAG PRIMER_RIGHT_10_SEQUENCE=GAATCACAAAGTGGGGATGG PRIMER_LEFT_10=454,20 PRIMER_RIGHT_10=539,20 PRIMER_LEFT_10_TM=59.592 PRIMER_RIGHT_10_TM=60.173 PRIMER_LEFT_10_GC_PERCENT=55.000 PRIMER_RIGHT_10_GC_PERCENT=50.000 PRIMER_LEFT_10_SELF_ANY=3.00 PRIMER_RIGHT_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=1.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_END_STABILITY=7.9000 PRIMER_RIGHT_10_END_STABILITY=8.1000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PAIR_10_PRODUCT_SIZE=86 PRIMER_PAIR_11_PENALTY=0.591934 PRIMER_LEFT_11_PENALTY=0.512286 PRIMER_RIGHT_11_PENALTY=0.079648 PRIMER_LEFT_11_SEQUENCE=TACCGCTGCTTAGTTGGTGA PRIMER_RIGHT_11_SEQUENCE=ACATCCATCCTCTCCTGGTG PRIMER_LEFT_11=542,20 PRIMER_RIGHT_11=627,20 PRIMER_LEFT_11_TM=59.488 PRIMER_RIGHT_11_TM=59.920 PRIMER_LEFT_11_GC_PERCENT=50.000 PRIMER_RIGHT_11_GC_PERCENT=55.000 PRIMER_LEFT_11_SELF_ANY=3.00 PRIMER_RIGHT_11_SELF_ANY=3.00 PRIMER_LEFT_11_SELF_END=1.00 PRIMER_RIGHT_11_SELF_END=3.00 PRIMER_LEFT_11_END_STABILITY=7.9000 PRIMER_RIGHT_11_END_STABILITY=8.2000 PRIMER_PAIR_11_COMPL_ANY=3.00 PRIMER_PAIR_11_COMPL_END=0.00 PRIMER_PAIR_11_PRODUCT_SIZE=86 PRIMER_PAIR_12_PENALTY=0.606764 PRIMER_LEFT_12_PENALTY=0.434141 PRIMER_RIGHT_12_PENALTY=0.172624 PRIMER_LEFT_12_SEQUENCE=CACCAATGTGGTAGAAGCCA PRIMER_RIGHT_12_SEQUENCE=GGAATCACAAAGTGGGGATG PRIMER_LEFT_12=445,20 PRIMER_RIGHT_12=540,20 PRIMER_LEFT_12_TM=59.566 PRIMER_RIGHT_12_TM=60.173 PRIMER_LEFT_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_GC_PERCENT=50.000 PRIMER_LEFT_12_SELF_ANY=6.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=1.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_END_STABILITY=9.7000 PRIMER_RIGHT_12_END_STABILITY=8.1000 PRIMER_PAIR_12_COMPL_ANY=4.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PAIR_12_PRODUCT_SIZE=96 PRIMER_PAIR_13_PENALTY=0.641488 PRIMER_LEFT_13_PENALTY=0.420643 PRIMER_RIGHT_13_PENALTY=0.220845 PRIMER_LEFT_13_SEQUENCE=ACTGATGGTAATGCTGGCCT PRIMER_RIGHT_13_SEQUENCE=CCACTTCCCATTCTGGACAT PRIMER_LEFT_13=263,20 PRIMER_RIGHT_13=355,20 PRIMER_LEFT_13_TM=59.579 PRIMER_RIGHT_13_TM=59.779 PRIMER_LEFT_13_GC_PERCENT=50.000 PRIMER_RIGHT_13_GC_PERCENT=50.000 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=3.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_END_STABILITY=10.9000 PRIMER_RIGHT_13_END_STABILITY=6.3000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PAIR_13_PRODUCT_SIZE=93 PRIMER_PAIR_14_PENALTY=0.642681 PRIMER_LEFT_14_PENALTY=0.215902 PRIMER_RIGHT_14_PENALTY=0.426779 PRIMER_LEFT_14_SEQUENCE=CCCCACTTTGTGATTCCCTA PRIMER_RIGHT_14_SEQUENCE=TTTGCACTTGTCAGGAACGA PRIMER_LEFT_14=524,20 PRIMER_RIGHT_14=601,20 PRIMER_LEFT_14_TM=59.784 PRIMER_RIGHT_14_TM=60.427 PRIMER_LEFT_14_GC_PERCENT=50.000 PRIMER_RIGHT_14_GC_PERCENT=45.000 PRIMER_LEFT_14_SELF_ANY=3.00 PRIMER_RIGHT_14_SELF_ANY=4.00 PRIMER_LEFT_14_SELF_END=3.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_END_STABILITY=8.7000 PRIMER_RIGHT_14_END_STABILITY=8.4000 PRIMER_PAIR_14_COMPL_ANY=4.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PAIR_14_PRODUCT_SIZE=78 PRIMER_PAIR_15_PENALTY=0.696384 PRIMER_LEFT_15_PENALTY=0.480482 PRIMER_RIGHT_15_PENALTY=0.215902 PRIMER_LEFT_15_SEQUENCE=CAACCAGTGACCATCCAGAA PRIMER_RIGHT_15_SEQUENCE=TAGGGAATCACAAAGTGGGG PRIMER_LEFT_15=467,20 PRIMER_RIGHT_15=543,20 PRIMER_LEFT_15_TM=59.520 PRIMER_RIGHT_15_TM=59.784 PRIMER_LEFT_15_GC_PERCENT=50.000 PRIMER_RIGHT_15_GC_PERCENT=50.000 PRIMER_LEFT_15_SELF_ANY=3.00 PRIMER_RIGHT_15_SELF_ANY=3.00 PRIMER_LEFT_15_SELF_END=1.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_END_STABILITY=7.0000 PRIMER_RIGHT_15_END_STABILITY=11.2000 PRIMER_PAIR_15_COMPL_ANY=4.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=77 PRIMER_PAIR_16_PENALTY=0.704589 PRIMER_LEFT_16_PENALTY=0.376749 PRIMER_RIGHT_16_PENALTY=0.327840 PRIMER_LEFT_16_SEQUENCE=CTGGAGGTACCCACTGATGG PRIMER_RIGHT_16_SEQUENCE=GTGCATGTTCAGTCTGCCAC PRIMER_LEFT_16=251,20 PRIMER_RIGHT_16=331,20 PRIMER_LEFT_16_TM=60.377 PRIMER_RIGHT_16_TM=60.328 PRIMER_LEFT_16_GC_PERCENT=60.000 PRIMER_RIGHT_16_GC_PERCENT=55.000 PRIMER_LEFT_16_SELF_ANY=6.00 PRIMER_RIGHT_16_SELF_ANY=5.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=3.00 PRIMER_LEFT_16_END_STABILITY=8.1000 PRIMER_RIGHT_16_END_STABILITY=9.4000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PAIR_16_PRODUCT_SIZE=81 PRIMER_PAIR_17_PENALTY=0.749777 PRIMER_LEFT_17_PENALTY=0.203367 PRIMER_RIGHT_17_PENALTY=0.546410 PRIMER_LEFT_17_SEQUENCE=GGACCAAAACCTGCATTGAT PRIMER_RIGHT_17_SEQUENCE=GGTTGGTTGGCTTCTACCAC PRIMER_LEFT_17=372,20 PRIMER_RIGHT_17=471,20 PRIMER_LEFT_17_TM=59.797 PRIMER_RIGHT_17_TM=59.454 PRIMER_LEFT_17_GC_PERCENT=45.000 PRIMER_RIGHT_17_GC_PERCENT=55.000 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=2.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_END_STABILITY=6.9000 PRIMER_RIGHT_17_END_STABILITY=7.6000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PAIR_17_PRODUCT_SIZE=100 PRIMER_PAIR_18_PENALTY=0.775672 PRIMER_LEFT_18_PENALTY=0.112124 PRIMER_RIGHT_18_PENALTY=0.663548 PRIMER_LEFT_18_SEQUENCE=CCAGATTGCCATGTTCTGTG PRIMER_RIGHT_18_SEQUENCE=TTTTGGTCCCTGATGGATCT PRIMER_LEFT_18=295,20 PRIMER_RIGHT_18=380,20 PRIMER_LEFT_18_TM=60.112 PRIMER_RIGHT_18_TM=59.336 PRIMER_LEFT_18_GC_PERCENT=50.000 PRIMER_RIGHT_18_GC_PERCENT=45.000 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=5.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=3.00 PRIMER_LEFT_18_END_STABILITY=6.7000 PRIMER_RIGHT_18_END_STABILITY=6.3000 PRIMER_PAIR_18_COMPL_ANY=4.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PAIR_18_PRODUCT_SIZE=86 PRIMER_PAIR_19_PENALTY=0.874857 PRIMER_LEFT_19_PENALTY=0.466916 PRIMER_RIGHT_19_PENALTY=0.407941 PRIMER_LEFT_19_SEQUENCE=GCATTGATACCAAGGAAGGC PRIMER_RIGHT_19_SEQUENCE=CTGGTTGGTTGGCTTCTACC PRIMER_LEFT_19=384,20 PRIMER_RIGHT_19=473,20 PRIMER_LEFT_19_TM=59.533 PRIMER_RIGHT_19_TM=59.592 PRIMER_LEFT_19_GC_PERCENT=50.000 PRIMER_RIGHT_19_GC_PERCENT=55.000 PRIMER_LEFT_19_SELF_ANY=6.00 PRIMER_RIGHT_19_SELF_ANY=3.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_END_STABILITY=9.7000 PRIMER_RIGHT_19_END_STABILITY=6.9000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=1.00 PRIMER_PAIR_19_PRODUCT_SIZE=90 PRIMER_PAIR_20_PENALTY=0.881003 PRIMER_LEFT_20_PENALTY=0.738752 PRIMER_RIGHT_20_PENALTY=0.142251 PRIMER_LEFT_20_SEQUENCE=CTCATCTTCACTGGCACACC PRIMER_RIGHT_20_SEQUENCE=AACATGCCGTAGTCATGCAA PRIMER_LEFT_20=636,20 PRIMER_RIGHT_20=711,20 PRIMER_LEFT_20_TM=59.261 PRIMER_RIGHT_20_TM=60.142 PRIMER_LEFT_20_GC_PERCENT=55.000 PRIMER_RIGHT_20_GC_PERCENT=45.000 PRIMER_LEFT_20_SELF_ANY=3.00 PRIMER_RIGHT_20_SELF_ANY=6.00 PRIMER_LEFT_20_SELF_END=0.00 PRIMER_RIGHT_20_SELF_END=2.00 PRIMER_LEFT_20_END_STABILITY=7.6000 PRIMER_RIGHT_20_END_STABILITY=8.8000 PRIMER_PAIR_20_COMPL_ANY=4.00 PRIMER_PAIR_20_COMPL_END=1.00 PRIMER_PAIR_20_PRODUCT_SIZE=76 PRIMER_PAIR_21_PENALTY=0.901425 PRIMER_LEFT_21_PENALTY=0.162673 PRIMER_RIGHT_21_PENALTY=0.738752 PRIMER_LEFT_21_SEQUENCE=CCCTTCTCGTTCCTGACAAG PRIMER_RIGHT_21_SEQUENCE=GGTGTGCCAGTGAAGATGAG PRIMER_LEFT_21=576,20 PRIMER_RIGHT_21=655,20 PRIMER_LEFT_21_TM=59.837 PRIMER_RIGHT_21_TM=59.261 PRIMER_LEFT_21_GC_PERCENT=55.000 PRIMER_RIGHT_21_GC_PERCENT=55.000 PRIMER_LEFT_21_SELF_ANY=4.00 PRIMER_RIGHT_21_SELF_ANY=3.00 PRIMER_LEFT_21_SELF_END=3.00 PRIMER_RIGHT_21_SELF_END=0.00 PRIMER_LEFT_21_END_STABILITY=6.7000 PRIMER_RIGHT_21_END_STABILITY=6.6000 PRIMER_PAIR_21_COMPL_ANY=5.00 PRIMER_PAIR_21_COMPL_END=3.00 PRIMER_PAIR_21_PRODUCT_SIZE=80 PRIMER_PAIR_22_PENALTY=0.953642 PRIMER_LEFT_22_PENALTY=0.554765 PRIMER_RIGHT_22_PENALTY=0.398877 PRIMER_LEFT_22_SEQUENCE=GAAGCCAACCAACCAGTGAC PRIMER_RIGHT_22_SEQUENCE=CAACTAAGCAGCGGTAGGGA PRIMER_LEFT_22=458,20 PRIMER_RIGHT_22=557,20 PRIMER_LEFT_22_TM=60.555 PRIMER_RIGHT_22_TM=60.399 PRIMER_LEFT_22_GC_PERCENT=55.000 PRIMER_RIGHT_22_GC_PERCENT=55.000 PRIMER_LEFT_22_SELF_ANY=3.00 PRIMER_RIGHT_22_SELF_ANY=3.00 PRIMER_LEFT_22_SELF_END=3.00 PRIMER_RIGHT_22_SELF_END=0.00 PRIMER_LEFT_22_END_STABILITY=6.1000 PRIMER_RIGHT_22_END_STABILITY=9.4000 PRIMER_PAIR_22_COMPL_ANY=4.00 PRIMER_PAIR_22_COMPL_END=1.00 PRIMER_PAIR_22_PRODUCT_SIZE=100 PRIMER_PAIR_23_PENALTY=1.005883 PRIMER_LEFT_23_PENALTY=0.532607 PRIMER_RIGHT_23_PENALTY=0.473276 PRIMER_LEFT_23_SEQUENCE=GCTGGAGGTACCCACTGATG PRIMER_RIGHT_23_SEQUENCE=GCATGTTCAGTCTGCCACAG PRIMER_LEFT_23=250,20 PRIMER_RIGHT_23=329,20 PRIMER_LEFT_23_TM=60.533 PRIMER_RIGHT_23_TM=60.473 PRIMER_LEFT_23_GC_PERCENT=60.000 PRIMER_RIGHT_23_GC_PERCENT=55.000 PRIMER_LEFT_23_SELF_ANY=6.00 PRIMER_RIGHT_23_SELF_ANY=5.00 PRIMER_LEFT_23_SELF_END=1.00 PRIMER_RIGHT_23_SELF_END=3.00 PRIMER_LEFT_23_END_STABILITY=6.9000 PRIMER_RIGHT_23_END_STABILITY=6.7000 PRIMER_PAIR_23_COMPL_ANY=5.00 PRIMER_PAIR_23_COMPL_END=3.00 PRIMER_PAIR_23_PRODUCT_SIZE=80 PRIMER_PAIR_24_PENALTY=1.153817 PRIMER_LEFT_24_PENALTY=0.546410 PRIMER_RIGHT_24_PENALTY=0.607407 PRIMER_LEFT_24_SEQUENCE=GTGGTAGAAGCCAACCAACC PRIMER_RIGHT_24_SEQUENCE=ACAAAGTGGGGATGGGTCTT PRIMER_LEFT_24=452,20 PRIMER_RIGHT_24=534,20 PRIMER_LEFT_24_TM=59.454 PRIMER_RIGHT_24_TM=60.607 PRIMER_LEFT_24_GC_PERCENT=55.000 PRIMER_RIGHT_24_GC_PERCENT=50.000 PRIMER_LEFT_24_SELF_ANY=4.00 PRIMER_RIGHT_24_SELF_ANY=3.00 PRIMER_LEFT_24_SELF_END=0.00 PRIMER_RIGHT_24_SELF_END=0.00 PRIMER_LEFT_24_END_STABILITY=8.2000 PRIMER_RIGHT_24_END_STABILITY=6.4000 PRIMER_PAIR_24_COMPL_ANY=4.00 PRIMER_PAIR_24_COMPL_END=2.00 PRIMER_PAIR_24_PRODUCT_SIZE=83 PRIMER_PAIR_25_PENALTY=1.160081 PRIMER_LEFT_25_PENALTY=0.906258 PRIMER_RIGHT_25_PENALTY=0.253823 PRIMER_LEFT_25_SEQUENCE=AGGAGAGGATGGATGTTTGC PRIMER_RIGHT_25_SEQUENCE=CATGCCGTAGTCATGCAAGT PRIMER_LEFT_25=612,20 PRIMER_RIGHT_25=709,20 PRIMER_LEFT_25_TM=59.094 PRIMER_RIGHT_25_TM=59.746 PRIMER_LEFT_25_GC_PERCENT=50.000 PRIMER_RIGHT_25_GC_PERCENT=50.000 PRIMER_LEFT_25_SELF_ANY=2.00 PRIMER_RIGHT_25_SELF_ANY=6.00 PRIMER_LEFT_25_SELF_END=2.00 PRIMER_RIGHT_25_SELF_END=2.00 PRIMER_LEFT_25_END_STABILITY=8.8000 PRIMER_RIGHT_25_END_STABILITY=6.7000 PRIMER_PAIR_25_COMPL_ANY=4.00 PRIMER_PAIR_25_COMPL_END=2.00 PRIMER_PAIR_25_PRODUCT_SIZE=98 PRIMER_PAIR_26_PENALTY=1.200572 PRIMER_LEFT_26_PENALTY=0.607407 PRIMER_RIGHT_26_PENALTY=0.593166 PRIMER_LEFT_26_SEQUENCE=AAGACCCATCCCCACTTTGT PRIMER_RIGHT_26_SEQUENCE=TGCACTTGTCAGGAACGAGA PRIMER_LEFT_26=515,20 PRIMER_RIGHT_26=599,20 PRIMER_LEFT_26_TM=60.607 PRIMER_RIGHT_26_TM=60.593 PRIMER_LEFT_26_GC_PERCENT=50.000 PRIMER_RIGHT_26_GC_PERCENT=50.000 PRIMER_LEFT_26_SELF_ANY=3.00 PRIMER_RIGHT_26_SELF_ANY=4.00 PRIMER_LEFT_26_SELF_END=1.00 PRIMER_RIGHT_26_SELF_END=0.00 PRIMER_LEFT_26_END_STABILITY=7.0000 PRIMER_RIGHT_26_END_STABILITY=8.4000 PRIMER_PAIR_26_COMPL_ANY=3.00 PRIMER_PAIR_26_COMPL_END=1.00 PRIMER_PAIR_26_PRODUCT_SIZE=85 PRIMER_PAIR_27_PENALTY=1.201833 PRIMER_LEFT_27_PENALTY=0.598673 PRIMER_RIGHT_27_PENALTY=0.603160 PRIMER_LEFT_27_SEQUENCE=CCTACCGCTGCTTAGTTGGT PRIMER_RIGHT_27_SEQUENCE=TTCGCAAACATCCATCCTCT PRIMER_LEFT_27=540,20 PRIMER_RIGHT_27=634,20 PRIMER_LEFT_27_TM=59.401 PRIMER_RIGHT_27_TM=60.603 PRIMER_LEFT_27_GC_PERCENT=55.000 PRIMER_RIGHT_27_GC_PERCENT=45.000 PRIMER_LEFT_27_SELF_ANY=3.00 PRIMER_RIGHT_27_SELF_ANY=4.00 PRIMER_LEFT_27_SELF_END=0.00 PRIMER_RIGHT_27_SELF_END=0.00 PRIMER_LEFT_27_END_STABILITY=8.2000 PRIMER_RIGHT_27_END_STABILITY=7.9000 PRIMER_PAIR_27_COMPL_ANY=3.00 PRIMER_PAIR_27_COMPL_END=1.00 PRIMER_PAIR_27_PRODUCT_SIZE=95 PRIMER_PAIR_28_PENALTY=1.276794 PRIMER_LEFT_28_PENALTY=0.137602 PRIMER_RIGHT_28_PENALTY=1.139192 PRIMER_LEFT_28_SEQUENCE=GGAAGTGGGATTCAGATCCA PRIMER_RIGHT_28_SEQUENCE=TTCAGGGTAGACTTCTTGGCA PRIMER_LEFT_28=348,20 PRIMER_RIGHT_28=436,21 PRIMER_LEFT_28_TM=59.862 PRIMER_RIGHT_28_TM=59.861 PRIMER_LEFT_28_GC_PERCENT=50.000 PRIMER_RIGHT_28_GC_PERCENT=47.619 PRIMER_LEFT_28_SELF_ANY=5.00 PRIMER_RIGHT_28_SELF_ANY=4.00 PRIMER_LEFT_28_SELF_END=3.00 PRIMER_RIGHT_28_SELF_END=3.00 PRIMER_LEFT_28_END_STABILITY=8.1000 PRIMER_RIGHT_28_END_STABILITY=10.0000 PRIMER_PAIR_28_COMPL_ANY=5.00 PRIMER_PAIR_28_COMPL_END=2.00 PRIMER_PAIR_28_PRODUCT_SIZE=89 PRIMER_PAIR_29_PENALTY=1.292454 PRIMER_LEFT_29_PENALTY=0.839509 PRIMER_RIGHT_29_PENALTY=0.452945 PRIMER_LEFT_29_SEQUENCE=ACCAACCAGTGACCATCCAG PRIMER_RIGHT_29_SEQUENCE=CTCACCAACTAAGCAGCGGT PRIMER_LEFT_29=465,20 PRIMER_RIGHT_29=562,20 PRIMER_LEFT_29_TM=60.840 PRIMER_RIGHT_29_TM=60.453 PRIMER_LEFT_29_GC_PERCENT=55.000 PRIMER_RIGHT_29_GC_PERCENT=55.000 PRIMER_LEFT_29_SELF_ANY=3.00 PRIMER_RIGHT_29_SELF_ANY=3.00 PRIMER_LEFT_29_SELF_END=1.00 PRIMER_RIGHT_29_SELF_END=2.00 PRIMER_LEFT_29_END_STABILITY=8.2000 PRIMER_RIGHT_29_END_STABILITY=11.1000 PRIMER_PAIR_29_COMPL_ANY=4.00 PRIMER_PAIR_29_COMPL_END=0.00 PRIMER_PAIR_29_PRODUCT_SIZE=98 PRIMER_PAIR_30_PENALTY=1.321678 PRIMER_LEFT_30_PENALTY=0.327840 PRIMER_RIGHT_30_PENALTY=0.993838 PRIMER_LEFT_30_SEQUENCE=GTGGCAGACTGAACATGCAC PRIMER_RIGHT_30_SEQUENCE=CAGGATGCCTTCCTTGGTAT PRIMER_LEFT_30=312,20 PRIMER_RIGHT_30=409,20 PRIMER_LEFT_30_TM=60.328 PRIMER_RIGHT_30_TM=59.006 PRIMER_LEFT_30_GC_PERCENT=55.000 PRIMER_RIGHT_30_GC_PERCENT=50.000 PRIMER_LEFT_30_SELF_ANY=5.00 PRIMER_RIGHT_30_SELF_ANY=7.00 PRIMER_LEFT_30_SELF_END=2.00 PRIMER_RIGHT_30_SELF_END=2.00 PRIMER_LEFT_30_END_STABILITY=8.2000 PRIMER_RIGHT_30_END_STABILITY=6.8000 PRIMER_PAIR_30_COMPL_ANY=5.00 PRIMER_PAIR_30_COMPL_END=1.00 PRIMER_PAIR_30_PRODUCT_SIZE=98 PRIMER_PAIR_31_PENALTY=1.325632 PRIMER_LEFT_31_PENALTY=1.188029 PRIMER_RIGHT_31_PENALTY=0.137602 PRIMER_LEFT_31_SEQUENCE=AACCCCAGATTGCCATGTT PRIMER_RIGHT_31_SEQUENCE=GATCTGAATCCCACTTCCCA PRIMER_LEFT_31=291,19 PRIMER_RIGHT_31=365,20 PRIMER_LEFT_31_TM=60.188 PRIMER_RIGHT_31_TM=59.862 PRIMER_LEFT_31_GC_PERCENT=47.368 PRIMER_RIGHT_31_GC_PERCENT=50.000 PRIMER_LEFT_31_SELF_ANY=4.00 PRIMER_RIGHT_31_SELF_ANY=4.00 PRIMER_LEFT_31_SELF_END=2.00 PRIMER_RIGHT_31_SELF_END=0.00 PRIMER_LEFT_31_END_STABILITY=6.6000 PRIMER_RIGHT_31_END_STABILITY=9.7000 PRIMER_PAIR_31_COMPL_ANY=5.00 PRIMER_PAIR_31_COMPL_END=1.00 PRIMER_PAIR_31_PRODUCT_SIZE=75 PRIMER_PAIR_32_PENALTY=1.421629 PRIMER_LEFT_32_PENALTY=0.689681 PRIMER_RIGHT_32_PENALTY=0.731948 PRIMER_LEFT_32_SEQUENCE=AGAAGCCAACCAACCAGTGA PRIMER_RIGHT_32_SEQUENCE=TAAGCAGCGGTAGGGAATCA PRIMER_LEFT_32=457,20 PRIMER_RIGHT_32=553,20 PRIMER_LEFT_32_TM=60.690 PRIMER_RIGHT_32_TM=60.732 PRIMER_LEFT_32_GC_PERCENT=50.000 PRIMER_RIGHT_32_GC_PERCENT=50.000 PRIMER_LEFT_32_SELF_ANY=3.00 PRIMER_RIGHT_32_SELF_ANY=3.00 PRIMER_LEFT_32_SELF_END=1.00 PRIMER_RIGHT_32_SELF_END=1.00 PRIMER_LEFT_32_END_STABILITY=6.4000 PRIMER_RIGHT_32_END_STABILITY=6.9000 PRIMER_PAIR_32_COMPL_ANY=4.00 PRIMER_PAIR_32_COMPL_END=3.00 PRIMER_PAIR_32_PRODUCT_SIZE=97 PRIMER_PAIR_33_PENALTY=1.426873 PRIMER_LEFT_33_PENALTY=0.426779 PRIMER_RIGHT_33_PENALTY=1.000094 PRIMER_LEFT_33_SEQUENCE=TCGTTCCTGACAAGTGCAAA PRIMER_RIGHT_33_SEQUENCE=ACTGCATGTCTCTTTGGCG PRIMER_LEFT_33=582,20 PRIMER_RIGHT_33=676,19 PRIMER_LEFT_33_TM=60.427 PRIMER_RIGHT_33_TM=60.000 PRIMER_LEFT_33_GC_PERCENT=45.000 PRIMER_RIGHT_33_GC_PERCENT=52.632 PRIMER_LEFT_33_SELF_ANY=4.00 PRIMER_RIGHT_33_SELF_ANY=4.00 PRIMER_LEFT_33_SELF_END=0.00 PRIMER_RIGHT_33_SELF_END=2.00 PRIMER_LEFT_33_END_STABILITY=8.8000 PRIMER_RIGHT_33_END_STABILITY=11.7000 PRIMER_PAIR_33_COMPL_ANY=4.00 PRIMER_PAIR_33_COMPL_END=2.00 PRIMER_PAIR_33_PRODUCT_SIZE=95 PRIMER_PAIR_34_PENALTY=1.429836 PRIMER_LEFT_34_PENALTY=0.079648 PRIMER_RIGHT_34_PENALTY=1.350189 PRIMER_LEFT_34_SEQUENCE=CACCAGGAGAGGATGGATGT PRIMER_RIGHT_34_SEQUENCE=CCGTAGTCATGCAAGTTGGT PRIMER_LEFT_34=608,20 PRIMER_RIGHT_34=705,20 PRIMER_LEFT_34_TM=59.920 PRIMER_RIGHT_34_TM=58.650 PRIMER_LEFT_34_GC_PERCENT=55.000 PRIMER_RIGHT_34_GC_PERCENT=50.000 PRIMER_LEFT_34_SELF_ANY=3.00 PRIMER_RIGHT_34_SELF_ANY=5.00 PRIMER_LEFT_34_SELF_END=0.00 PRIMER_RIGHT_34_SELF_END=1.00 PRIMER_LEFT_34_END_STABILITY=6.3000 PRIMER_RIGHT_34_END_STABILITY=8.2000 PRIMER_PAIR_34_COMPL_ANY=4.00 PRIMER_PAIR_34_COMPL_END=0.00 PRIMER_PAIR_34_PRODUCT_SIZE=98 PRIMER_PAIR_35_PENALTY=1.515297 PRIMER_LEFT_35_PENALTY=1.062353 PRIMER_RIGHT_35_PENALTY=0.452945 PRIMER_LEFT_35_SEQUENCE=CCAACCAGTGACCATCCAG PRIMER_RIGHT_35_SEQUENCE=ACTCACCAACTAAGCAGCGG PRIMER_LEFT_35=466,19 PRIMER_RIGHT_35=563,20 PRIMER_LEFT_35_TM=59.938 PRIMER_RIGHT_35_TM=60.453 PRIMER_LEFT_35_GC_PERCENT=57.895 PRIMER_RIGHT_35_GC_PERCENT=55.000 PRIMER_LEFT_35_SELF_ANY=3.00 PRIMER_RIGHT_35_SELF_ANY=3.00 PRIMER_LEFT_35_SELF_END=1.00 PRIMER_RIGHT_35_SELF_END=2.00 PRIMER_LEFT_35_END_STABILITY=8.2000 PRIMER_RIGHT_35_END_STABILITY=11.4000 PRIMER_PAIR_35_COMPL_ANY=4.00 PRIMER_PAIR_35_COMPL_END=1.00 PRIMER_PAIR_35_PRODUCT_SIZE=98 PRIMER_PAIR_36_PENALTY=1.554865 PRIMER_LEFT_36_PENALTY=0.473276 PRIMER_RIGHT_36_PENALTY=1.081589 PRIMER_LEFT_36_SEQUENCE=CTGTGGCAGACTGAACATGC PRIMER_RIGHT_36_SEQUENCE=GCAGGTTTTGGTCCCTGAT PRIMER_LEFT_36=310,20 PRIMER_RIGHT_36=385,19 PRIMER_LEFT_36_TM=60.473 PRIMER_RIGHT_36_TM=59.918 PRIMER_LEFT_36_GC_PERCENT=55.000 PRIMER_RIGHT_36_GC_PERCENT=52.632 PRIMER_LEFT_36_SELF_ANY=5.00 PRIMER_RIGHT_36_SELF_ANY=4.00 PRIMER_LEFT_36_SELF_END=2.00 PRIMER_RIGHT_36_SELF_END=2.00 PRIMER_LEFT_36_END_STABILITY=8.4000 PRIMER_RIGHT_36_END_STABILITY=6.6000 PRIMER_PAIR_36_COMPL_ANY=5.00 PRIMER_PAIR_36_COMPL_END=1.00 PRIMER_PAIR_36_PRODUCT_SIZE=76 PRIMER_PAIR_37_PENALTY=1.638206 PRIMER_LEFT_37_PENALTY=0.731948 PRIMER_RIGHT_37_PENALTY=0.906258 PRIMER_LEFT_37_SEQUENCE=TGATTCCCTACCGCTGCTTA PRIMER_RIGHT_37_SEQUENCE=GCAAACATCCATCCTCTCCT PRIMER_LEFT_37=534,20 PRIMER_RIGHT_37=631,20 PRIMER_LEFT_37_TM=60.732 PRIMER_RIGHT_37_TM=59.094 PRIMER_LEFT_37_GC_PERCENT=50.000 PRIMER_RIGHT_37_GC_PERCENT=50.000 PRIMER_LEFT_37_SELF_ANY=3.00 PRIMER_RIGHT_37_SELF_ANY=2.00 PRIMER_LEFT_37_SELF_END=2.00 PRIMER_RIGHT_37_SELF_END=0.00 PRIMER_LEFT_37_END_STABILITY=7.5000 PRIMER_RIGHT_37_END_STABILITY=7.9000 PRIMER_PAIR_37_COMPL_ANY=3.00 PRIMER_PAIR_37_COMPL_END=1.00 PRIMER_PAIR_37_PRODUCT_SIZE=98 PRIMER_PAIR_38_PENALTY=1.710319 PRIMER_LEFT_38_PENALTY=0.079648 PRIMER_RIGHT_38_PENALTY=1.630671 PRIMER_LEFT_38_SEQUENCE=ACACCAGGAGAGGATGGATG PRIMER_RIGHT_38_SEQUENCE=GCCGTAGTCATGCAAGTTGG PRIMER_LEFT_38=607,20 PRIMER_RIGHT_38=706,20 PRIMER_LEFT_38_TM=59.920 PRIMER_RIGHT_38_TM=61.631 PRIMER_LEFT_38_GC_PERCENT=55.000 PRIMER_RIGHT_38_GC_PERCENT=55.000 PRIMER_LEFT_38_SELF_ANY=3.00 PRIMER_RIGHT_38_SELF_ANY=5.00 PRIMER_LEFT_38_SELF_END=0.00 PRIMER_RIGHT_38_SELF_END=3.00 PRIMER_LEFT_38_END_STABILITY=8.1000 PRIMER_RIGHT_38_END_STABILITY=8.2000 PRIMER_PAIR_38_COMPL_ANY=4.00 PRIMER_PAIR_38_COMPL_END=0.00 PRIMER_PAIR_38_PRODUCT_SIZE=100 PRIMER_PAIR_39_PENALTY=1.727747 PRIMER_LEFT_39_PENALTY=1.215461 PRIMER_RIGHT_39_PENALTY=0.512286 PRIMER_LEFT_39_SEQUENCE=ACCAACCAGTGACCATCCA PRIMER_RIGHT_39_SEQUENCE=TCACCAACTAAGCAGCGGTA PRIMER_LEFT_39=465,19 PRIMER_RIGHT_39=561,20 PRIMER_LEFT_39_TM=59.785 PRIMER_RIGHT_39_TM=59.488 PRIMER_LEFT_39_GC_PERCENT=52.632 PRIMER_RIGHT_39_GC_PERCENT=50.000 PRIMER_LEFT_39_SELF_ANY=3.00 PRIMER_RIGHT_39_SELF_ANY=3.00 PRIMER_LEFT_39_SELF_END=0.00 PRIMER_RIGHT_39_SELF_END=2.00 PRIMER_LEFT_39_END_STABILITY=8.1000 PRIMER_RIGHT_39_END_STABILITY=8.9000 PRIMER_PAIR_39_COMPL_ANY=4.00 PRIMER_PAIR_39_COMPL_END=0.00 PRIMER_PAIR_39_PRODUCT_SIZE=97 PRIMER_PAIR_40_PENALTY=1.751977 PRIMER_LEFT_40_PENALTY=0.923785 PRIMER_RIGHT_40_PENALTY=0.828193 PRIMER_LEFT_40_SEQUENCE=CCCAGATTGCCATGTTCTGT PRIMER_RIGHT_40_SEQUENCE=CCTGATGGATCTGAATCCCA PRIMER_LEFT_40=294,20 PRIMER_RIGHT_40=372,20 PRIMER_LEFT_40_TM=60.924 PRIMER_RIGHT_40_TM=60.828 PRIMER_LEFT_40_GC_PERCENT=50.000 PRIMER_RIGHT_40_GC_PERCENT=50.000 PRIMER_LEFT_40_SELF_ANY=4.00 PRIMER_RIGHT_40_SELF_ANY=5.00 PRIMER_LEFT_40_SELF_END=0.00 PRIMER_RIGHT_40_SELF_END=3.00 PRIMER_LEFT_40_END_STABILITY=6.4000 PRIMER_RIGHT_40_END_STABILITY=9.7000 PRIMER_PAIR_40_COMPL_ANY=5.00 PRIMER_PAIR_40_COMPL_END=1.00 PRIMER_PAIR_40_PRODUCT_SIZE=79 PRIMER_PAIR_41_PENALTY=1.782319 PRIMER_LEFT_41_PENALTY=1.146286 PRIMER_RIGHT_41_PENALTY=0.636033 PRIMER_LEFT_41_SEQUENCE=ATCTTCACTGGCACACCGTC PRIMER_RIGHT_41_SEQUENCE=GAACTTGTCAATTCCGCAGG PRIMER_LEFT_41=639,20 PRIMER_RIGHT_41=736,20 PRIMER_LEFT_41_TM=61.146 PRIMER_RIGHT_41_TM=60.636 PRIMER_LEFT_41_GC_PERCENT=55.000 PRIMER_RIGHT_41_GC_PERCENT=50.000 PRIMER_LEFT_41_SELF_ANY=3.00 PRIMER_RIGHT_41_SELF_ANY=7.00 PRIMER_LEFT_41_SELF_END=1.00 PRIMER_RIGHT_41_SELF_END=2.00 PRIMER_LEFT_41_END_STABILITY=9.6000 PRIMER_RIGHT_41_END_STABILITY=9.7000 PRIMER_PAIR_41_COMPL_ANY=3.00 PRIMER_PAIR_41_COMPL_END=1.00 PRIMER_PAIR_41_PRODUCT_SIZE=98 PRIMER_PAIR_42_PENALTY=1.821407 PRIMER_LEFT_42_PENALTY=1.683804 PRIMER_RIGHT_42_PENALTY=0.137602 PRIMER_LEFT_42_SEQUENCE=ACCCCAGATTGCCATGTTCT PRIMER_RIGHT_42_SEQUENCE=TGGATCTGAATCCCACTTCC PRIMER_LEFT_42=292,20 PRIMER_RIGHT_42=367,20 PRIMER_LEFT_42_TM=61.684 PRIMER_RIGHT_42_TM=59.862 PRIMER_LEFT_42_GC_PERCENT=50.000 PRIMER_RIGHT_42_GC_PERCENT=50.000 PRIMER_LEFT_42_SELF_ANY=4.00 PRIMER_RIGHT_42_SELF_ANY=5.00 PRIMER_LEFT_42_SELF_END=0.00 PRIMER_RIGHT_42_SELF_END=2.00 PRIMER_LEFT_42_END_STABILITY=6.4000 PRIMER_RIGHT_42_END_STABILITY=8.2000 PRIMER_PAIR_42_COMPL_ANY=5.00 PRIMER_PAIR_42_COMPL_END=0.00 PRIMER_PAIR_42_PRODUCT_SIZE=76 PRIMER_PAIR_43_PENALTY=1.836755 PRIMER_LEFT_43_PENALTY=1.238082 PRIMER_RIGHT_43_PENALTY=0.598673 PRIMER_LEFT_43_SEQUENCE=AACCAACCAGTGACCATCCA PRIMER_RIGHT_43_SEQUENCE=ACCAACTAAGCAGCGGTAGG PRIMER_LEFT_43=464,20 PRIMER_RIGHT_43=559,20 PRIMER_LEFT_43_TM=61.238 PRIMER_RIGHT_43_TM=59.401 PRIMER_LEFT_43_GC_PERCENT=50.000 PRIMER_RIGHT_43_GC_PERCENT=55.000 PRIMER_LEFT_43_SELF_ANY=3.00 PRIMER_RIGHT_43_SELF_ANY=3.00 PRIMER_LEFT_43_SELF_END=0.00 PRIMER_RIGHT_43_SELF_END=1.00 PRIMER_LEFT_43_END_STABILITY=8.1000 PRIMER_RIGHT_43_END_STABILITY=6.9000 PRIMER_PAIR_43_COMPL_ANY=4.00 PRIMER_PAIR_43_COMPL_END=1.00 PRIMER_PAIR_43_PRODUCT_SIZE=96 PRIMER_PAIR_44_PENALTY=1.858077 PRIMER_LEFT_44_PENALTY=0.711791 PRIMER_RIGHT_44_PENALTY=1.146286 PRIMER_LEFT_44_SEQUENCE=AAGTGATGCCCTTCTCGTTC PRIMER_RIGHT_44_SEQUENCE=GACGGTGTGCCAGTGAAGAT PRIMER_LEFT_44=568,20 PRIMER_RIGHT_44=658,20 PRIMER_LEFT_44_TM=59.288 PRIMER_RIGHT_44_TM=61.146 PRIMER_LEFT_44_GC_PERCENT=50.000 PRIMER_RIGHT_44_GC_PERCENT=55.000 PRIMER_LEFT_44_SELF_ANY=4.00 PRIMER_RIGHT_44_SELF_ANY=3.00 PRIMER_LEFT_44_SELF_END=0.00 PRIMER_RIGHT_44_SELF_END=2.00 PRIMER_LEFT_44_END_STABILITY=8.4000 PRIMER_RIGHT_44_END_STABILITY=6.6000 PRIMER_PAIR_44_COMPL_ANY=4.00 PRIMER_PAIR_44_COMPL_END=2.00 PRIMER_PAIR_44_PRODUCT_SIZE=91 PRIMER_PAIR_45_PENALTY=1.870574 PRIMER_LEFT_45_PENALTY=0.805475 PRIMER_RIGHT_45_PENALTY=1.065099 PRIMER_LEFT_45_SEQUENCE=CTTTGTGATTCCCTACCGCT PRIMER_RIGHT_45_SEQUENCE=CATCCATCCTCTCCTGGTGTA PRIMER_LEFT_45=529,20 PRIMER_RIGHT_45=626,21 PRIMER_LEFT_45_TM=59.195 PRIMER_RIGHT_45_TM=59.935 PRIMER_LEFT_45_GC_PERCENT=50.000 PRIMER_RIGHT_45_GC_PERCENT=52.381 PRIMER_LEFT_45_SELF_ANY=3.00 PRIMER_RIGHT_45_SELF_ANY=3.00 PRIMER_LEFT_45_SELF_END=2.00 PRIMER_RIGHT_45_SELF_END=2.00 PRIMER_LEFT_45_END_STABILITY=11.4000 PRIMER_RIGHT_45_END_STABILITY=5.4000 PRIMER_PAIR_45_COMPL_ANY=3.00 PRIMER_PAIR_45_COMPL_END=1.00 PRIMER_PAIR_45_PRODUCT_SIZE=98 PRIMER_PAIR_46_PENALTY=1.873083 PRIMER_LEFT_46_PENALTY=1.392601 PRIMER_RIGHT_46_PENALTY=0.480482 PRIMER_LEFT_46_SEQUENCE=TCCTGCAGTATTGCCAAGAAG PRIMER_RIGHT_46_SEQUENCE=TTCTGGATGGTCACTGGTTG PRIMER_LEFT_46=405,21 PRIMER_RIGHT_46=486,20 PRIMER_LEFT_46_TM=60.393 PRIMER_RIGHT_46_TM=59.520 PRIMER_LEFT_46_GC_PERCENT=47.619 PRIMER_RIGHT_46_GC_PERCENT=50.000 PRIMER_LEFT_46_SELF_ANY=6.00 PRIMER_RIGHT_46_SELF_ANY=3.00 PRIMER_LEFT_46_SELF_END=1.00 PRIMER_RIGHT_46_SELF_END=0.00 PRIMER_LEFT_46_END_STABILITY=6.7000 PRIMER_RIGHT_46_END_STABILITY=8.2000 PRIMER_PAIR_46_COMPL_ANY=4.00 PRIMER_PAIR_46_COMPL_END=3.00 PRIMER_PAIR_46_PRODUCT_SIZE=82 PRIMER_PAIR_47_PENALTY=1.939458 PRIMER_LEFT_47_PENALTY=0.949861 PRIMER_RIGHT_47_PENALTY=0.989597 PRIMER_LEFT_47_SEQUENCE=ATCCCCACTTTGTGATTCCC PRIMER_RIGHT_47_SEQUENCE=GCACTTGTCAGGAACGAGAA PRIMER_LEFT_47=522,20 PRIMER_RIGHT_47=598,20 PRIMER_LEFT_47_TM=60.950 PRIMER_RIGHT_47_TM=59.010 PRIMER_LEFT_47_GC_PERCENT=50.000 PRIMER_RIGHT_47_GC_PERCENT=50.000 PRIMER_LEFT_47_SELF_ANY=4.00 PRIMER_RIGHT_47_SELF_ANY=3.00 PRIMER_LEFT_47_SELF_END=3.00 PRIMER_RIGHT_47_SELF_END=1.00 PRIMER_LEFT_47_END_STABILITY=9.7000 PRIMER_RIGHT_47_END_STABILITY=6.7000 PRIMER_PAIR_47_COMPL_ANY=4.00 PRIMER_PAIR_47_COMPL_END=3.00 PRIMER_PAIR_47_PRODUCT_SIZE=77 PRIMER_PAIR_48_PENALTY=1.982572 PRIMER_LEFT_48_PENALTY=1.427808 PRIMER_RIGHT_48_PENALTY=0.554765 PRIMER_LEFT_48_SEQUENCE=TGCATTGATACCAAGGAAGG PRIMER_RIGHT_48_SEQUENCE=GTCACTGGTTGGTTGGCTTC PRIMER_LEFT_48=383,20 PRIMER_RIGHT_48=477,20 PRIMER_LEFT_48_TM=58.572 PRIMER_RIGHT_48_TM=60.555 PRIMER_LEFT_48_GC_PERCENT=45.000 PRIMER_RIGHT_48_GC_PERCENT=55.000 PRIMER_LEFT_48_SELF_ANY=6.00 PRIMER_RIGHT_48_SELF_ANY=3.00 PRIMER_LEFT_48_SELF_END=0.00 PRIMER_RIGHT_48_SELF_END=0.00 PRIMER_LEFT_48_END_STABILITY=8.2000 PRIMER_RIGHT_48_END_STABILITY=8.2000 PRIMER_PAIR_48_COMPL_ANY=5.00 PRIMER_PAIR_48_COMPL_END=3.00 PRIMER_PAIR_48_PRODUCT_SIZE=95 PRIMER_PAIR_49_PENALTY=1.985862 PRIMER_LEFT_49_PENALTY=1.003259 PRIMER_RIGHT_49_PENALTY=0.982603 PRIMER_LEFT_49_SEQUENCE=TTCCCTACCGCTGCTTAGTT PRIMER_RIGHT_49_SEQUENCE=TTTCGCAAACATCCATCCTC PRIMER_LEFT_49=537,20 PRIMER_RIGHT_49=635,20 PRIMER_LEFT_49_TM=58.997 PRIMER_RIGHT_49_TM=60.983 PRIMER_LEFT_49_GC_PERCENT=50.000 PRIMER_RIGHT_49_GC_PERCENT=45.000 PRIMER_LEFT_49_SELF_ANY=3.00 PRIMER_RIGHT_49_SELF_ANY=4.00 PRIMER_LEFT_49_SELF_END=0.00 PRIMER_RIGHT_49_SELF_END=0.00 PRIMER_LEFT_49_END_STABILITY=5.7000 PRIMER_RIGHT_49_END_STABILITY=7.9000 PRIMER_PAIR_49_COMPL_ANY=3.00 PRIMER_PAIR_49_COMPL_END=0.00 PRIMER_PAIR_49_PRODUCT_SIZE=99 PRIMER_PAIR_50_PENALTY=1.999512 PRIMER_LEFT_50_PENALTY=1.126243 PRIMER_RIGHT_50_PENALTY=0.873270 PRIMER_LEFT_50_SEQUENCE=TGTAAGTGATGCCCTTCTCG PRIMER_RIGHT_50_SEQUENCE=TGAGTTTCGCAAACATCCAT PRIMER_LEFT_50=565,20 PRIMER_RIGHT_50=639,20 PRIMER_LEFT_50_TM=58.874 PRIMER_RIGHT_50_TM=59.127 PRIMER_LEFT_50_GC_PERCENT=50.000 PRIMER_RIGHT_50_GC_PERCENT=40.000 PRIMER_LEFT_50_SELF_ANY=4.00 PRIMER_RIGHT_50_SELF_ANY=6.00 PRIMER_LEFT_50_SELF_END=2.00 PRIMER_RIGHT_50_SELF_END=2.00 PRIMER_LEFT_50_END_STABILITY=8.4000 PRIMER_RIGHT_50_END_STABILITY=8.1000 PRIMER_PAIR_50_COMPL_ANY=4.00 PRIMER_PAIR_50_COMPL_END=1.00 PRIMER_PAIR_50_PRODUCT_SIZE=75 PRIMER_PAIR_51_PENALTY=2.005671 PRIMER_LEFT_51_PENALTY=1.055809 PRIMER_RIGHT_51_PENALTY=0.949861 PRIMER_LEFT_51_SEQUENCE=CCCAGATTGCCATGTTCTG PRIMER_RIGHT_51_SEQUENCE=GGTTTTGGTCCCTGATGGAT PRIMER_LEFT_51=294,19 PRIMER_RIGHT_51=382,20 PRIMER_LEFT_51_TM=60.056 PRIMER_RIGHT_51_TM=60.950 PRIMER_LEFT_51_GC_PERCENT=52.632 PRIMER_RIGHT_51_GC_PERCENT=50.000 PRIMER_LEFT_51_SELF_ANY=4.00 PRIMER_RIGHT_51_SELF_ANY=5.00 PRIMER_LEFT_51_SELF_END=1.00 PRIMER_RIGHT_51_SELF_END=3.00 PRIMER_LEFT_51_END_STABILITY=7.0000 PRIMER_RIGHT_51_END_STABILITY=8.1000 PRIMER_PAIR_51_COMPL_ANY=4.00 PRIMER_PAIR_51_COMPL_END=0.00 PRIMER_PAIR_51_PRODUCT_SIZE=89 PRIMER_PAIR_52_PENALTY=2.032732 PRIMER_LEFT_52_PENALTY=1.227257 PRIMER_RIGHT_52_PENALTY=0.805475 PRIMER_LEFT_52_SEQUENCE=TAGAAGCCAACCAACCAGTG PRIMER_RIGHT_52_SEQUENCE=AGCGGTAGGGAATCACAAAG PRIMER_LEFT_52=456,20 PRIMER_RIGHT_52=548,20 PRIMER_LEFT_52_TM=58.773 PRIMER_RIGHT_52_TM=59.195 PRIMER_LEFT_52_GC_PERCENT=50.000 PRIMER_RIGHT_52_GC_PERCENT=50.000 PRIMER_LEFT_52_SELF_ANY=3.00 PRIMER_RIGHT_52_SELF_ANY=3.00 PRIMER_LEFT_52_SELF_END=3.00 PRIMER_RIGHT_52_SELF_END=0.00 PRIMER_LEFT_52_END_STABILITY=6.7000 PRIMER_RIGHT_52_END_STABILITY=7.3000 PRIMER_PAIR_52_COMPL_ANY=4.00 PRIMER_PAIR_52_COMPL_END=1.00 PRIMER_PAIR_52_PRODUCT_SIZE=93 PRIMER_PAIR_53_PENALTY=2.041918 PRIMER_LEFT_53_PENALTY=1.139192 PRIMER_RIGHT_53_PENALTY=0.902727 PRIMER_LEFT_53_SEQUENCE=TGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_53_SEQUENCE=CCAGTTCTGGATGGTCACTG PRIMER_LEFT_53=416,21 PRIMER_RIGHT_53=490,20 PRIMER_LEFT_53_TM=59.861 PRIMER_RIGHT_53_TM=59.097 PRIMER_LEFT_53_GC_PERCENT=47.619 PRIMER_RIGHT_53_GC_PERCENT=55.000 PRIMER_LEFT_53_SELF_ANY=4.00 PRIMER_RIGHT_53_SELF_ANY=6.00 PRIMER_LEFT_53_SELF_END=0.00 PRIMER_RIGHT_53_SELF_END=3.00 PRIMER_LEFT_53_END_STABILITY=7.0000 PRIMER_RIGHT_53_END_STABILITY=6.7000 PRIMER_PAIR_53_COMPL_ANY=4.00 PRIMER_PAIR_53_COMPL_END=2.00 PRIMER_PAIR_53_PRODUCT_SIZE=75 PRIMER_PAIR_54_PENALTY=2.041918 PRIMER_LEFT_54_PENALTY=1.139192 PRIMER_RIGHT_54_PENALTY=0.902727 PRIMER_LEFT_54_SEQUENCE=TTGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_54_SEQUENCE=CAGTTCTGGATGGTCACTGG PRIMER_LEFT_54=415,21 PRIMER_RIGHT_54=489,20 PRIMER_LEFT_54_TM=59.861 PRIMER_RIGHT_54_TM=59.097 PRIMER_LEFT_54_GC_PERCENT=47.619 PRIMER_RIGHT_54_GC_PERCENT=55.000 PRIMER_LEFT_54_SELF_ANY=5.00 PRIMER_RIGHT_54_SELF_ANY=4.00 PRIMER_LEFT_54_SELF_END=1.00 PRIMER_RIGHT_54_SELF_END=1.00 PRIMER_LEFT_54_END_STABILITY=8.2000 PRIMER_RIGHT_54_END_STABILITY=7.9000 PRIMER_PAIR_54_COMPL_ANY=4.00 PRIMER_PAIR_54_COMPL_END=2.00 PRIMER_PAIR_54_PRODUCT_SIZE=75 PRIMER_PAIR_55_PENALTY=2.053793 PRIMER_LEFT_55_PENALTY=1.950354 PRIMER_RIGHT_55_PENALTY=0.103439 PRIMER_LEFT_55_SEQUENCE=GTCGCCAAAGAGACATGCAG PRIMER_RIGHT_55_SEQUENCE=CACAAACTCTACCCCTCGGA PRIMER_LEFT_55=656,20 PRIMER_RIGHT_55=754,20 PRIMER_LEFT_55_TM=61.950 PRIMER_RIGHT_55_TM=60.103 PRIMER_LEFT_55_GC_PERCENT=55.000 PRIMER_RIGHT_55_GC_PERCENT=55.000 PRIMER_LEFT_55_SELF_ANY=4.00 PRIMER_RIGHT_55_SELF_ANY=3.00 PRIMER_LEFT_55_SELF_END=2.00 PRIMER_RIGHT_55_SELF_END=3.00 PRIMER_LEFT_55_END_STABILITY=8.5000 PRIMER_RIGHT_55_END_STABILITY=9.9000 PRIMER_PAIR_55_COMPL_ANY=4.00 PRIMER_PAIR_55_COMPL_END=1.00 PRIMER_PAIR_55_PRODUCT_SIZE=99 PRIMER_PAIR_56_PENALTY=2.080242 PRIMER_LEFT_56_PENALTY=1.020705 PRIMER_RIGHT_56_PENALTY=1.059537 PRIMER_LEFT_56_SEQUENCE=TTTGTGATTCCCTACCGCTG PRIMER_RIGHT_56_SEQUENCE=AAACATCCATCCTCTCCTGG PRIMER_LEFT_56=530,20 PRIMER_RIGHT_56=629,20 PRIMER_LEFT_56_TM=61.021 PRIMER_RIGHT_56_TM=58.940 PRIMER_LEFT_56_GC_PERCENT=50.000 PRIMER_RIGHT_56_GC_PERCENT=50.000 PRIMER_LEFT_56_SELF_ANY=3.00 PRIMER_RIGHT_56_SELF_ANY=3.00 PRIMER_LEFT_56_SELF_END=2.00 PRIMER_RIGHT_56_SELF_END=3.00 PRIMER_LEFT_56_END_STABILITY=10.2000 PRIMER_RIGHT_56_END_STABILITY=9.7000 PRIMER_PAIR_56_COMPL_ANY=3.00 PRIMER_PAIR_56_COMPL_END=0.00 PRIMER_PAIR_56_PRODUCT_SIZE=100 PRIMER_PAIR_57_PENALTY=2.085972 PRIMER_LEFT_57_PENALTY=1.071784 PRIMER_RIGHT_57_PENALTY=1.014189 PRIMER_LEFT_57_SEQUENCE=GATTGCCATGTTCTGTGGC PRIMER_RIGHT_57_SEQUENCE=TGGTCCCTGATGGATCTGA PRIMER_LEFT_57=298,19 PRIMER_RIGHT_57=377,19 PRIMER_LEFT_57_TM=60.072 PRIMER_RIGHT_57_TM=59.986 PRIMER_LEFT_57_GC_PERCENT=52.632 PRIMER_RIGHT_57_GC_PERCENT=52.632 PRIMER_LEFT_57_SELF_ANY=4.00 PRIMER_RIGHT_57_SELF_ANY=5.00 PRIMER_LEFT_57_SELF_END=2.00 PRIMER_RIGHT_57_SELF_END=3.00 PRIMER_LEFT_57_END_STABILITY=9.4000 PRIMER_RIGHT_57_END_STABILITY=6.7000 PRIMER_PAIR_57_COMPL_ANY=4.00 PRIMER_PAIR_57_COMPL_END=0.00 PRIMER_PAIR_57_PRODUCT_SIZE=80 PRIMER_PAIR_58_PENALTY=2.105553 PRIMER_LEFT_58_PENALTY=0.944463 PRIMER_RIGHT_58_PENALTY=1.161090 PRIMER_LEFT_58_SEQUENCE=ACTTTGTGATTCCCTACCGC PRIMER_RIGHT_58_SEQUENCE=AATTTGCACTTGTCAGGAACG PRIMER_LEFT_58=528,20 PRIMER_RIGHT_58=603,21 PRIMER_LEFT_58_TM=59.056 PRIMER_RIGHT_58_TM=60.161 PRIMER_LEFT_58_GC_PERCENT=50.000 PRIMER_RIGHT_58_GC_PERCENT=42.857 PRIMER_LEFT_58_SELF_ANY=3.00 PRIMER_RIGHT_58_SELF_ANY=4.00 PRIMER_LEFT_58_SELF_END=2.00 PRIMER_RIGHT_58_SELF_END=2.00 PRIMER_LEFT_58_END_STABILITY=11.1000 PRIMER_RIGHT_58_END_STABILITY=8.4000 PRIMER_PAIR_58_COMPL_ANY=4.00 PRIMER_PAIR_58_COMPL_END=1.00 PRIMER_PAIR_58_PRODUCT_SIZE=76 PRIMER_PAIR_59_PENALTY=2.131956 PRIMER_LEFT_59_PENALTY=1.468408 PRIMER_RIGHT_59_PENALTY=0.663548 PRIMER_LEFT_59_SEQUENCE=CCACTGATGGTAATGCTGGC PRIMER_RIGHT_59_SEQUENCE=GAATCCCACTTCCCATTCTG PRIMER_LEFT_59=261,20 PRIMER_RIGHT_59=360,20 PRIMER_LEFT_59_TM=61.468 PRIMER_RIGHT_59_TM=59.336 PRIMER_LEFT_59_GC_PERCENT=55.000 PRIMER_RIGHT_59_GC_PERCENT=50.000 PRIMER_LEFT_59_SELF_ANY=3.00 PRIMER_RIGHT_59_SELF_ANY=4.00 PRIMER_LEFT_59_SELF_END=2.00 PRIMER_RIGHT_59_SELF_END=1.00 PRIMER_LEFT_59_END_STABILITY=9.7000 PRIMER_RIGHT_59_END_STABILITY=7.0000 PRIMER_PAIR_59_COMPL_ANY=5.00 PRIMER_PAIR_59_COMPL_END=3.00 PRIMER_PAIR_59_PRODUCT_SIZE=100 PRIMER_PAIR_60_PENALTY=2.164052 PRIMER_LEFT_60_PENALTY=0.989597 PRIMER_RIGHT_60_PENALTY=1.174455 PRIMER_LEFT_60_SEQUENCE=TTCTCGTTCCTGACAAGTGC PRIMER_RIGHT_60_SEQUENCE=GGTGTGCCAGTGAAGATGAGT PRIMER_LEFT_60=579,20 PRIMER_RIGHT_60=655,21 PRIMER_LEFT_60_TM=59.010 PRIMER_RIGHT_60_TM=60.174 PRIMER_LEFT_60_GC_PERCENT=50.000 PRIMER_RIGHT_60_GC_PERCENT=52.381 PRIMER_LEFT_60_SELF_ANY=3.00 PRIMER_RIGHT_60_SELF_ANY=3.00 PRIMER_LEFT_60_SELF_END=2.00 PRIMER_RIGHT_60_SELF_END=1.00 PRIMER_LEFT_60_END_STABILITY=7.9000 PRIMER_RIGHT_60_END_STABILITY=6.4000 PRIMER_PAIR_60_COMPL_ANY=5.00 PRIMER_PAIR_60_COMPL_END=0.00 PRIMER_PAIR_60_PRODUCT_SIZE=77 PRIMER_PAIR_61_PENALTY=2.182545 PRIMER_LEFT_61_PENALTY=1.238082 PRIMER_RIGHT_61_PENALTY=0.944463 PRIMER_LEFT_61_SEQUENCE=CAACCAACCAGTGACCATCC PRIMER_RIGHT_61_SEQUENCE=GCGGTAGGGAATCACAAAGT PRIMER_LEFT_61=463,20 PRIMER_RIGHT_61=547,20 PRIMER_LEFT_61_TM=61.238 PRIMER_RIGHT_61_TM=59.056 PRIMER_LEFT_61_GC_PERCENT=55.000 PRIMER_RIGHT_61_GC_PERCENT=50.000 PRIMER_LEFT_61_SELF_ANY=3.00 PRIMER_RIGHT_61_SELF_ANY=3.00 PRIMER_LEFT_61_SELF_END=0.00 PRIMER_RIGHT_61_SELF_END=1.00 PRIMER_LEFT_61_END_STABILITY=8.1000 PRIMER_RIGHT_61_END_STABILITY=6.7000 PRIMER_PAIR_61_COMPL_ANY=4.00 PRIMER_PAIR_61_COMPL_END=0.00 PRIMER_PAIR_61_PRODUCT_SIZE=85 PRIMER_PAIR_62_PENALTY=2.196647 PRIMER_LEFT_62_PENALTY=1.153556 PRIMER_RIGHT_62_PENALTY=1.043090 PRIMER_LEFT_62_SEQUENCE=CAAGCAGTGCAAGACCCAT PRIMER_RIGHT_62_SEQUENCE=GCACTTGTCAGGAACGAGAAG PRIMER_LEFT_62=505,19 PRIMER_RIGHT_62=598,21 PRIMER_LEFT_62_TM=59.846 PRIMER_RIGHT_62_TM=60.043 PRIMER_LEFT_62_GC_PERCENT=52.632 PRIMER_RIGHT_62_GC_PERCENT=52.381 PRIMER_LEFT_62_SELF_ANY=5.00 PRIMER_RIGHT_62_SELF_ANY=4.00 PRIMER_LEFT_62_SELF_END=2.00 PRIMER_RIGHT_62_SELF_END=3.00 PRIMER_LEFT_62_END_STABILITY=9.6000 PRIMER_RIGHT_62_END_STABILITY=6.7000 PRIMER_PAIR_62_COMPL_ANY=5.00 PRIMER_PAIR_62_COMPL_END=1.00 PRIMER_PAIR_62_PRODUCT_SIZE=94 PRIMER_PAIR_63_PENALTY=2.207651 PRIMER_LEFT_63_PENALTY=1.048185 PRIMER_RIGHT_63_PENALTY=1.159466 PRIMER_LEFT_63_SEQUENCE=GATTCCCTACCGCTGCTTAG PRIMER_RIGHT_63_SEQUENCE=TCGCAAACATCCATCCTCTC PRIMER_LEFT_63=535,20 PRIMER_RIGHT_63=633,20 PRIMER_LEFT_63_TM=58.952 PRIMER_RIGHT_63_TM=61.159 PRIMER_LEFT_63_GC_PERCENT=55.000 PRIMER_RIGHT_63_GC_PERCENT=50.000 PRIMER_LEFT_63_SELF_ANY=3.00 PRIMER_RIGHT_63_SELF_ANY=2.00 PRIMER_LEFT_63_SELF_END=3.00 PRIMER_RIGHT_63_SELF_END=0.00 PRIMER_LEFT_63_END_STABILITY=6.0000 PRIMER_RIGHT_63_END_STABILITY=6.4000 PRIMER_PAIR_63_COMPL_ANY=3.00 PRIMER_PAIR_63_COMPL_END=1.00 PRIMER_PAIR_63_PRODUCT_SIZE=99 PRIMER_PAIR_64_PENALTY=2.221779 PRIMER_LEFT_64_PENALTY=0.663548 PRIMER_RIGHT_64_PENALTY=1.558232 PRIMER_LEFT_64_SEQUENCE=AGATCCATCAGGGACCAAAA PRIMER_RIGHT_64_SEQUENCE=TCAGGGTAGACTTCTTGGCA PRIMER_LEFT_64=361,20 PRIMER_RIGHT_64=435,20 PRIMER_LEFT_64_TM=59.336 PRIMER_RIGHT_64_TM=58.442 PRIMER_LEFT_64_GC_PERCENT=45.000 PRIMER_RIGHT_64_GC_PERCENT=50.000 PRIMER_LEFT_64_SELF_ANY=5.00 PRIMER_RIGHT_64_SELF_ANY=4.00 PRIMER_LEFT_64_SELF_END=0.00 PRIMER_RIGHT_64_SELF_END=3.00 PRIMER_LEFT_64_END_STABILITY=7.6000 PRIMER_RIGHT_64_END_STABILITY=10.0000 PRIMER_PAIR_64_COMPL_ANY=4.00 PRIMER_PAIR_64_COMPL_END=2.00 PRIMER_PAIR_64_PRODUCT_SIZE=75 PRIMER_PAIR_65_PENALTY=2.236534 PRIMER_LEFT_65_PENALTY=1.011619 PRIMER_RIGHT_65_PENALTY=1.224915 PRIMER_LEFT_65_SEQUENCE=ACTTTGTGATTCCCTACCGCT PRIMER_RIGHT_65_SEQUENCE=ATTTGCACTTGTCAGGAACG PRIMER_LEFT_65=528,21 PRIMER_RIGHT_65=602,20 PRIMER_LEFT_65_TM=60.012 PRIMER_RIGHT_65_TM=58.775 PRIMER_LEFT_65_GC_PERCENT=47.619 PRIMER_RIGHT_65_GC_PERCENT=45.000 PRIMER_LEFT_65_SELF_ANY=3.00 PRIMER_RIGHT_65_SELF_ANY=4.00 PRIMER_LEFT_65_SELF_END=2.00 PRIMER_RIGHT_65_SELF_END=2.00 PRIMER_LEFT_65_END_STABILITY=11.4000 PRIMER_RIGHT_65_END_STABILITY=8.4000 PRIMER_PAIR_65_COMPL_ANY=4.00 PRIMER_PAIR_65_COMPL_END=2.00 PRIMER_PAIR_65_PRODUCT_SIZE=75 PRIMER_PAIR_66_PENALTY=2.241341 PRIMER_LEFT_66_PENALTY=1.238082 PRIMER_RIGHT_66_PENALTY=1.003259 PRIMER_LEFT_66_SEQUENCE=CCAACCAACCAGTGACCATC PRIMER_RIGHT_66_SEQUENCE=AACTAAGCAGCGGTAGGGAA PRIMER_LEFT_66=462,20 PRIMER_RIGHT_66=556,20 PRIMER_LEFT_66_TM=61.238 PRIMER_RIGHT_66_TM=58.997 PRIMER_LEFT_66_GC_PERCENT=55.000 PRIMER_RIGHT_66_GC_PERCENT=50.000 PRIMER_LEFT_66_SELF_ANY=3.00 PRIMER_RIGHT_66_SELF_ANY=3.00 PRIMER_LEFT_66_SELF_END=1.00 PRIMER_RIGHT_66_SELF_END=0.00 PRIMER_LEFT_66_END_STABILITY=8.1000 PRIMER_RIGHT_66_END_STABILITY=9.7000 PRIMER_PAIR_66_COMPL_ANY=4.00 PRIMER_PAIR_66_COMPL_END=1.00 PRIMER_PAIR_66_PRODUCT_SIZE=95 PRIMER_PAIR_67_PENALTY=2.261575 PRIMER_LEFT_67_PENALTY=1.078278 PRIMER_RIGHT_67_PENALTY=1.183296 PRIMER_LEFT_67_SEQUENCE=AGATTGCCATGTTCTGTGGC PRIMER_RIGHT_67_SEQUENCE=GTTTTGGTCCCTGATGGATCT PRIMER_LEFT_67=297,20 PRIMER_RIGHT_67=381,21 PRIMER_LEFT_67_TM=61.078 PRIMER_RIGHT_67_TM=60.183 PRIMER_LEFT_67_GC_PERCENT=50.000 PRIMER_RIGHT_67_GC_PERCENT=47.619 PRIMER_LEFT_67_SELF_ANY=4.00 PRIMER_RIGHT_67_SELF_ANY=5.00 PRIMER_LEFT_67_SELF_END=2.00 PRIMER_RIGHT_67_SELF_END=3.00 PRIMER_LEFT_67_END_STABILITY=9.4000 PRIMER_RIGHT_67_END_STABILITY=6.3000 PRIMER_PAIR_67_COMPL_ANY=4.00 PRIMER_PAIR_67_COMPL_END=0.00 PRIMER_PAIR_67_PRODUCT_SIZE=85 PRIMER_PAIR_68_PENALTY=2.267317 PRIMER_LEFT_68_PENALTY=1.427808 PRIMER_RIGHT_68_PENALTY=0.839509 PRIMER_LEFT_68_SEQUENCE=CATTGATACCAAGGAAGGCA PRIMER_RIGHT_68_SEQUENCE=CTGGATGGTCACTGGTTGGT PRIMER_LEFT_68=385,20 PRIMER_RIGHT_68=484,20 PRIMER_LEFT_68_TM=58.572 PRIMER_RIGHT_68_TM=60.840 PRIMER_LEFT_68_GC_PERCENT=45.000 PRIMER_RIGHT_68_GC_PERCENT=55.000 PRIMER_LEFT_68_SELF_ANY=6.00 PRIMER_RIGHT_68_SELF_ANY=3.00 PRIMER_LEFT_68_SELF_END=0.00 PRIMER_RIGHT_68_SELF_END=0.00 PRIMER_LEFT_68_END_STABILITY=9.7000 PRIMER_RIGHT_68_END_STABILITY=8.2000 PRIMER_PAIR_68_COMPL_ANY=5.00 PRIMER_PAIR_68_COMPL_END=2.00 PRIMER_PAIR_68_PRODUCT_SIZE=100 PRIMER_PAIR_69_PENALTY=2.343809 PRIMER_LEFT_69_PENALTY=1.236064 PRIMER_RIGHT_69_PENALTY=1.107745 PRIMER_LEFT_69_SEQUENCE=AAGACCCATCCCCACTTTG PRIMER_RIGHT_69_SEQUENCE=CACTTGTCAGGAACGAGAAGG PRIMER_LEFT_69=515,19 PRIMER_RIGHT_69=597,21 PRIMER_LEFT_69_TM=59.764 PRIMER_RIGHT_69_TM=59.892 PRIMER_LEFT_69_GC_PERCENT=52.632 PRIMER_RIGHT_69_GC_PERCENT=52.381 PRIMER_LEFT_69_SELF_ANY=3.00 PRIMER_RIGHT_69_SELF_ANY=4.00 PRIMER_LEFT_69_SELF_END=1.00 PRIMER_RIGHT_69_SELF_END=1.00 PRIMER_LEFT_69_END_STABILITY=7.3000 PRIMER_RIGHT_69_END_STABILITY=8.2000 PRIMER_PAIR_69_COMPL_ANY=3.00 PRIMER_PAIR_69_COMPL_END=1.00 PRIMER_PAIR_69_PRODUCT_SIZE=83 PRIMER_PAIR_70_PENALTY=2.345473 PRIMER_LEFT_70_PENALTY=1.333854 PRIMER_RIGHT_70_PENALTY=1.011619 PRIMER_LEFT_70_SEQUENCE=GAAGCCAACCAACCAGTGA PRIMER_RIGHT_70_SEQUENCE=AGCGGTAGGGAATCACAAAGT PRIMER_LEFT_70=458,19 PRIMER_RIGHT_70=548,21 PRIMER_LEFT_70_TM=59.666 PRIMER_RIGHT_70_TM=60.012 PRIMER_LEFT_70_GC_PERCENT=52.632 PRIMER_RIGHT_70_GC_PERCENT=47.619 PRIMER_LEFT_70_SELF_ANY=3.00 PRIMER_RIGHT_70_SELF_ANY=3.00 PRIMER_LEFT_70_SELF_END=1.00 PRIMER_RIGHT_70_SELF_END=1.00 PRIMER_LEFT_70_END_STABILITY=6.4000 PRIMER_RIGHT_70_END_STABILITY=6.7000 PRIMER_PAIR_70_COMPL_ANY=4.00 PRIMER_PAIR_70_COMPL_END=2.00 PRIMER_PAIR_70_PRODUCT_SIZE=91 PRIMER_PAIR_71_PENALTY=2.373120 PRIMER_LEFT_71_PENALTY=1.352415 PRIMER_RIGHT_71_PENALTY=1.020705 PRIMER_LEFT_71_SEQUENCE=GTAGAAGCCAACCAACCAGTG PRIMER_RIGHT_71_SEQUENCE=CAGCGGTAGGGAATCACAAA PRIMER_LEFT_71=455,21 PRIMER_RIGHT_71=549,20 PRIMER_LEFT_71_TM=59.648 PRIMER_RIGHT_71_TM=61.021 PRIMER_LEFT_71_GC_PERCENT=52.381 PRIMER_RIGHT_71_GC_PERCENT=50.000 PRIMER_LEFT_71_SELF_ANY=3.00 PRIMER_RIGHT_71_SELF_ANY=3.00 PRIMER_LEFT_71_SELF_END=3.00 PRIMER_RIGHT_71_SELF_END=1.00 PRIMER_LEFT_71_END_STABILITY=6.7000 PRIMER_RIGHT_71_END_STABILITY=7.0000 PRIMER_PAIR_71_COMPL_ANY=4.00 PRIMER_PAIR_71_COMPL_END=0.00 PRIMER_PAIR_71_PRODUCT_SIZE=95 PRIMER_PAIR_72_PENALTY=2.402627 PRIMER_LEFT_72_PENALTY=1.741437 PRIMER_RIGHT_72_PENALTY=0.661190 PRIMER_LEFT_72_SEQUENCE=ACCCCAGATTGCCATGTTC PRIMER_RIGHT_72_SEQUENCE=GGATCTGAATCCCACTTCCC PRIMER_LEFT_72=292,19 PRIMER_RIGHT_72=366,20 PRIMER_LEFT_72_TM=60.741 PRIMER_RIGHT_72_TM=60.661 PRIMER_LEFT_72_GC_PERCENT=52.632 PRIMER_RIGHT_72_GC_PERCENT=55.000 PRIMER_LEFT_72_SELF_ANY=4.00 PRIMER_RIGHT_72_SELF_ANY=5.00 PRIMER_LEFT_72_SELF_END=0.00 PRIMER_RIGHT_72_SELF_END=0.00 PRIMER_LEFT_72_END_STABILITY=6.7000 PRIMER_RIGHT_72_END_STABILITY=9.7000 PRIMER_PAIR_72_COMPL_ANY=5.00 PRIMER_PAIR_72_COMPL_END=0.00 PRIMER_PAIR_72_PRODUCT_SIZE=75 PRIMER_PAIR_73_PENALTY=2.417959 PRIMER_LEFT_73_PENALTY=1.672380 PRIMER_RIGHT_73_PENALTY=0.745579 PRIMER_LEFT_73_SEQUENCE=ACCGTCGCCAAAGAGACAT PRIMER_RIGHT_73_SEQUENCE=ACCCCTCGGAACTTGTCAAT PRIMER_LEFT_73=653,19 PRIMER_RIGHT_73=744,20 PRIMER_LEFT_73_TM=60.672 PRIMER_RIGHT_73_TM=60.746 PRIMER_LEFT_73_GC_PERCENT=52.632 PRIMER_RIGHT_73_GC_PERCENT=50.000 PRIMER_LEFT_73_SELF_ANY=3.00 PRIMER_RIGHT_73_SELF_ANY=5.00 PRIMER_LEFT_73_SELF_END=2.00 PRIMER_RIGHT_73_SELF_END=3.00 PRIMER_LEFT_73_END_STABILITY=6.3000 PRIMER_RIGHT_73_END_STABILITY=6.9000 PRIMER_PAIR_73_COMPL_ANY=4.00 PRIMER_PAIR_73_COMPL_END=2.00 PRIMER_PAIR_73_PRODUCT_SIZE=92 PRIMER_PAIR_74_PENALTY=2.424239 PRIMER_LEFT_74_PENALTY=1.233331 PRIMER_RIGHT_74_PENALTY=1.190908 PRIMER_LEFT_74_SEQUENCE=GTGATTCCCTACCGCTGCT PRIMER_RIGHT_74_SEQUENCE=AAACATCCATCCTCTCCTGGT PRIMER_LEFT_74=533,19 PRIMER_RIGHT_74=629,21 PRIMER_LEFT_74_TM=60.233 PRIMER_RIGHT_74_TM=59.809 PRIMER_LEFT_74_GC_PERCENT=57.895 PRIMER_RIGHT_74_GC_PERCENT=47.619 PRIMER_LEFT_74_SELF_ANY=3.00 PRIMER_RIGHT_74_SELF_ANY=3.00 PRIMER_LEFT_74_SELF_END=1.00 PRIMER_RIGHT_74_SELF_END=1.00 PRIMER_LEFT_74_END_STABILITY=8.2000 PRIMER_RIGHT_74_END_STABILITY=7.9000 PRIMER_PAIR_74_COMPL_ANY=3.00 PRIMER_PAIR_74_COMPL_END=0.00 PRIMER_PAIR_74_PRODUCT_SIZE=97 PRIMER_PAIR_75_PENALTY=2.456656 PRIMER_LEFT_75_PENALTY=1.271611 PRIMER_RIGHT_75_PENALTY=1.185045 PRIMER_LEFT_75_SEQUENCE=GCCATGTTCTGTGGCAGAC PRIMER_RIGHT_75_SEQUENCE=TATCAATGCAGGTTTTGGTCC PRIMER_LEFT_75=302,19 PRIMER_RIGHT_75=392,21 PRIMER_LEFT_75_TM=60.272 PRIMER_RIGHT_75_TM=59.815 PRIMER_LEFT_75_GC_PERCENT=57.895 PRIMER_RIGHT_75_GC_PERCENT=42.857 PRIMER_LEFT_75_SELF_ANY=5.00 PRIMER_RIGHT_75_SELF_ANY=4.00 PRIMER_LEFT_75_SELF_END=3.00 PRIMER_RIGHT_75_SELF_END=3.00 PRIMER_LEFT_75_END_STABILITY=6.4000 PRIMER_RIGHT_75_END_STABILITY=9.1000 PRIMER_PAIR_75_COMPL_ANY=3.00 PRIMER_PAIR_75_COMPL_END=2.00 PRIMER_PAIR_75_PRODUCT_SIZE=91 PRIMER_PAIR_76_PENALTY=2.463300 PRIMER_LEFT_76_PENALTY=1.415114 PRIMER_RIGHT_76_PENALTY=1.048185 PRIMER_LEFT_76_SEQUENCE=CAACCAGTGACCATCCAGAAC PRIMER_RIGHT_76_SEQUENCE=CTAAGCAGCGGTAGGGAATC PRIMER_LEFT_76=467,21 PRIMER_RIGHT_76=554,20 PRIMER_LEFT_76_TM=60.415 PRIMER_RIGHT_76_TM=58.952 PRIMER_LEFT_76_GC_PERCENT=52.381 PRIMER_RIGHT_76_GC_PERCENT=55.000 PRIMER_LEFT_76_SELF_ANY=3.00 PRIMER_RIGHT_76_SELF_ANY=3.00 PRIMER_LEFT_76_SELF_END=0.00 PRIMER_RIGHT_76_SELF_END=3.00 PRIMER_LEFT_76_END_STABILITY=6.4000 PRIMER_RIGHT_76_END_STABILITY=6.6000 PRIMER_PAIR_76_COMPL_ANY=3.00 PRIMER_PAIR_76_COMPL_END=0.00 PRIMER_PAIR_76_PRODUCT_SIZE=88 PRIMER_PAIR_77_PENALTY=2.490026 PRIMER_LEFT_77_PENALTY=1.472425 PRIMER_RIGHT_77_PENALTY=1.017601 PRIMER_LEFT_77_SEQUENCE=CCTGCAGTATTGCCAAGAAG PRIMER_RIGHT_77_SEQUENCE=CAGTTCTGGATGGTCACTGGT PRIMER_LEFT_77=406,20 PRIMER_RIGHT_77=489,21 PRIMER_LEFT_77_TM=58.528 PRIMER_RIGHT_77_TM=60.018 PRIMER_LEFT_77_GC_PERCENT=50.000 PRIMER_RIGHT_77_GC_PERCENT=52.381 PRIMER_LEFT_77_SELF_ANY=6.00 PRIMER_RIGHT_77_SELF_ANY=4.00 PRIMER_LEFT_77_SELF_END=1.00 PRIMER_RIGHT_77_SELF_END=2.00 PRIMER_LEFT_77_END_STABILITY=6.7000 PRIMER_RIGHT_77_END_STABILITY=7.9000 PRIMER_PAIR_77_COMPL_ANY=4.00 PRIMER_PAIR_77_COMPL_END=1.00 PRIMER_PAIR_77_PRODUCT_SIZE=84 PRIMER_PAIR_78_PENALTY=2.500188 PRIMER_LEFT_78_PENALTY=1.316198 PRIMER_RIGHT_78_PENALTY=1.183989 PRIMER_LEFT_78_SEQUENCE=CCCACTGATGGTAATGCTGG PRIMER_RIGHT_78_SEQUENCE=CATGTGCATGTTCAGTCTGC PRIMER_LEFT_78=260,20 PRIMER_RIGHT_78=334,20 PRIMER_LEFT_78_TM=61.316 PRIMER_RIGHT_78_TM=58.816 PRIMER_LEFT_78_GC_PERCENT=55.000 PRIMER_RIGHT_78_GC_PERCENT=50.000 PRIMER_LEFT_78_SELF_ANY=3.00 PRIMER_RIGHT_78_SELF_ANY=6.00 PRIMER_LEFT_78_SELF_END=0.00 PRIMER_RIGHT_78_SELF_END=3.00 PRIMER_LEFT_78_END_STABILITY=9.7000 PRIMER_RIGHT_78_END_STABILITY=8.2000 PRIMER_PAIR_78_COMPL_ANY=5.00 PRIMER_PAIR_78_COMPL_END=1.00 PRIMER_PAIR_78_PRODUCT_SIZE=75 PRIMER_PAIR_79_PENALTY=2.528184 PRIMER_LEFT_79_PENALTY=1.292120 PRIMER_RIGHT_79_PENALTY=1.236064 PRIMER_LEFT_79_SEQUENCE=GCCATGTTCTGTGGCAGACT PRIMER_RIGHT_79_SEQUENCE=AGGTTTTGGTCCCTGATGG PRIMER_LEFT_79=302,20 PRIMER_RIGHT_79=383,19 PRIMER_LEFT_79_TM=61.292 PRIMER_RIGHT_79_TM=59.764 PRIMER_LEFT_79_GC_PERCENT=55.000 PRIMER_RIGHT_79_GC_PERCENT=52.632 PRIMER_LEFT_79_SELF_ANY=5.00 PRIMER_RIGHT_79_SELF_ANY=3.00 PRIMER_LEFT_79_SELF_END=3.00 PRIMER_RIGHT_79_SELF_END=3.00 PRIMER_LEFT_79_END_STABILITY=6.1000 PRIMER_RIGHT_79_END_STABILITY=8.1000 PRIMER_PAIR_79_COMPL_ANY=4.00 PRIMER_PAIR_79_COMPL_END=0.00 PRIMER_PAIR_79_PRODUCT_SIZE=82 PRIMER_PAIR_80_PENALTY=2.535486 PRIMER_LEFT_80_PENALTY=0.704204 PRIMER_RIGHT_80_PENALTY=1.831282 PRIMER_LEFT_80_SEQUENCE=CATGAATGTCCAGAATGGGA PRIMER_RIGHT_80_SEQUENCE=CAGGATGCCTTCCTTGGTATC PRIMER_LEFT_80=331,20 PRIMER_RIGHT_80=409,21 PRIMER_LEFT_80_TM=59.296 PRIMER_RIGHT_80_TM=60.831 PRIMER_LEFT_80_GC_PERCENT=45.000 PRIMER_RIGHT_80_GC_PERCENT=52.381 PRIMER_LEFT_80_SELF_ANY=4.00 PRIMER_RIGHT_80_SELF_ANY=7.00 PRIMER_LEFT_80_SELF_END=1.00 PRIMER_RIGHT_80_SELF_END=2.00 PRIMER_LEFT_80_END_STABILITY=9.7000 PRIMER_RIGHT_80_END_STABILITY=5.3000 PRIMER_PAIR_80_COMPL_ANY=3.00 PRIMER_PAIR_80_COMPL_END=2.00 PRIMER_PAIR_80_PRODUCT_SIZE=79 PRIMER_PAIR_81_PENALTY=2.562163 PRIMER_LEFT_81_PENALTY=1.185045 PRIMER_RIGHT_81_PENALTY=1.377118 PRIMER_LEFT_81_SEQUENCE=GGACCAAAACCTGCATTGATA PRIMER_RIGHT_81_SEQUENCE=GTTGGTTGGCTTCTACCACA PRIMER_LEFT_81=372,21 PRIMER_RIGHT_81=470,20 PRIMER_LEFT_81_TM=59.815 PRIMER_RIGHT_81_TM=58.623 PRIMER_LEFT_81_GC_PERCENT=42.857 PRIMER_RIGHT_81_GC_PERCENT=50.000 PRIMER_LEFT_81_SELF_ANY=4.00 PRIMER_RIGHT_81_SELF_ANY=4.00 PRIMER_LEFT_81_SELF_END=2.00 PRIMER_RIGHT_81_SELF_END=2.00 PRIMER_LEFT_81_END_STABILITY=5.9000 PRIMER_RIGHT_81_END_STABILITY=8.2000 PRIMER_PAIR_81_COMPL_ANY=5.00 PRIMER_PAIR_81_COMPL_END=2.00 PRIMER_PAIR_81_PRODUCT_SIZE=99 PRIMER_PAIR_82_PENALTY=2.575832 PRIMER_LEFT_82_PENALTY=1.558232 PRIMER_RIGHT_82_PENALTY=1.017601 PRIMER_LEFT_82_SEQUENCE=TGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_82_SEQUENCE=ACCAGTTCTGGATGGTCACTG PRIMER_LEFT_82=416,20 PRIMER_RIGHT_82=491,21 PRIMER_LEFT_82_TM=58.442 PRIMER_RIGHT_82_TM=60.018 PRIMER_LEFT_82_GC_PERCENT=50.000 PRIMER_RIGHT_82_GC_PERCENT=52.381 PRIMER_LEFT_82_SELF_ANY=4.00 PRIMER_RIGHT_82_SELF_ANY=6.00 PRIMER_LEFT_82_SELF_END=1.00 PRIMER_RIGHT_82_SELF_END=3.00 PRIMER_LEFT_82_END_STABILITY=8.2000 PRIMER_RIGHT_82_END_STABILITY=6.7000 PRIMER_PAIR_82_COMPL_ANY=4.00 PRIMER_PAIR_82_COMPL_END=2.00 PRIMER_PAIR_82_PRODUCT_SIZE=76 PRIMER_PAIR_83_PENALTY=2.583424 PRIMER_LEFT_83_PENALTY=1.429867 PRIMER_RIGHT_83_PENALTY=1.153556 PRIMER_LEFT_83_SEQUENCE=TCACCAATGTGGTAGAAGCC PRIMER_RIGHT_83_SEQUENCE=ATGGGTCTTGCACTGCTTG PRIMER_LEFT_83=444,20 PRIMER_RIGHT_83=523,19 PRIMER_LEFT_83_TM=58.570 PRIMER_RIGHT_83_TM=59.846 PRIMER_LEFT_83_GC_PERCENT=50.000 PRIMER_RIGHT_83_GC_PERCENT=52.632 PRIMER_LEFT_83_SELF_ANY=6.00 PRIMER_RIGHT_83_SELF_ANY=5.00 PRIMER_LEFT_83_SELF_END=1.00 PRIMER_RIGHT_83_SELF_END=3.00 PRIMER_LEFT_83_END_STABILITY=9.7000 PRIMER_RIGHT_83_END_STABILITY=8.5000 PRIMER_PAIR_83_COMPL_ANY=4.00 PRIMER_PAIR_83_COMPL_END=3.00 PRIMER_PAIR_83_PRODUCT_SIZE=80 PRIMER_PAIR_84_PENALTY=2.613176 PRIMER_LEFT_84_PENALTY=1.174455 PRIMER_RIGHT_84_PENALTY=1.438721 PRIMER_LEFT_84_SEQUENCE=ACTCATCTTCACTGGCACACC PRIMER_RIGHT_84_SEQUENCE=GCAGCAACATGCCGTAGTC PRIMER_LEFT_84=635,21 PRIMER_RIGHT_84=716,19 PRIMER_LEFT_84_TM=60.174 PRIMER_RIGHT_84_TM=60.439 PRIMER_LEFT_84_GC_PERCENT=52.381 PRIMER_RIGHT_84_GC_PERCENT=57.895 PRIMER_LEFT_84_SELF_ANY=3.00 PRIMER_RIGHT_84_SELF_ANY=4.00 PRIMER_LEFT_84_SELF_END=0.00 PRIMER_RIGHT_84_SELF_END=1.00 PRIMER_LEFT_84_END_STABILITY=7.6000 PRIMER_RIGHT_84_END_STABILITY=5.4000 PRIMER_PAIR_84_COMPL_ANY=4.00 PRIMER_PAIR_84_COMPL_END=0.00 PRIMER_PAIR_84_PRODUCT_SIZE=82 PRIMER_PAIR_85_PENALTY=2.613182 PRIMER_LEFT_85_PENALTY=1.377118 PRIMER_RIGHT_85_PENALTY=1.236064 PRIMER_LEFT_85_SEQUENCE=TGTGGTAGAAGCCAACCAAC PRIMER_RIGHT_85_SEQUENCE=AAAGTGGGGATGGGTCTTG PRIMER_LEFT_85=451,20 PRIMER_RIGHT_85=532,19 PRIMER_LEFT_85_TM=58.623 PRIMER_RIGHT_85_TM=59.764 PRIMER_LEFT_85_GC_PERCENT=50.000 PRIMER_RIGHT_85_GC_PERCENT=52.632 PRIMER_LEFT_85_SELF_ANY=4.00 PRIMER_RIGHT_85_SELF_ANY=3.00 PRIMER_LEFT_85_SELF_END=2.00 PRIMER_RIGHT_85_SELF_END=0.00 PRIMER_LEFT_85_END_STABILITY=8.2000 PRIMER_RIGHT_85_END_STABILITY=7.0000 PRIMER_PAIR_85_COMPL_ANY=4.00 PRIMER_PAIR_85_COMPL_END=2.00 PRIMER_PAIR_85_PRODUCT_SIZE=82 PRIMER_PAIR_86_PENALTY=2.625605 PRIMER_LEFT_86_PENALTY=1.608005 PRIMER_RIGHT_86_PENALTY=1.017601 PRIMER_LEFT_86_SEQUENCE=CCTGCAGTATTGCCAAGAAGT PRIMER_RIGHT_86_SEQUENCE=CACCAGTTCTGGATGGTCACT PRIMER_LEFT_86=406,21 PRIMER_RIGHT_86=492,21 PRIMER_LEFT_86_TM=59.392 PRIMER_RIGHT_86_TM=60.018 PRIMER_LEFT_86_GC_PERCENT=47.619 PRIMER_RIGHT_86_GC_PERCENT=52.381 PRIMER_LEFT_86_SELF_ANY=6.00 PRIMER_RIGHT_86_SELF_ANY=6.00 PRIMER_LEFT_86_SELF_END=1.00 PRIMER_RIGHT_86_SELF_END=1.00 PRIMER_LEFT_86_END_STABILITY=6.4000 PRIMER_RIGHT_86_END_STABILITY=6.4000 PRIMER_PAIR_86_COMPL_ANY=4.00 PRIMER_PAIR_86_COMPL_END=3.00 PRIMER_PAIR_86_PRODUCT_SIZE=87 PRIMER_PAIR_87_PENALTY=2.642454 PRIMER_LEFT_87_PENALTY=1.409123 PRIMER_RIGHT_87_PENALTY=1.233331 PRIMER_LEFT_87_SEQUENCE=GGTAGAAGCCAACCAACCAGT PRIMER_RIGHT_87_SEQUENCE=AGCAGCGGTAGGGAATCAC PRIMER_LEFT_87=454,21 PRIMER_RIGHT_87=551,19 PRIMER_LEFT_87_TM=60.409 PRIMER_RIGHT_87_TM=60.233 PRIMER_LEFT_87_GC_PERCENT=52.381 PRIMER_RIGHT_87_GC_PERCENT=57.895 PRIMER_LEFT_87_SELF_ANY=3.00 PRIMER_RIGHT_87_SELF_ANY=3.00 PRIMER_LEFT_87_SELF_END=2.00 PRIMER_RIGHT_87_SELF_END=3.00 PRIMER_LEFT_87_END_STABILITY=7.9000 PRIMER_RIGHT_87_END_STABILITY=6.3000 PRIMER_PAIR_87_COMPL_ANY=4.00 PRIMER_PAIR_87_COMPL_END=2.00 PRIMER_PAIR_87_PRODUCT_SIZE=98 PRIMER_PAIR_88_PENALTY=2.645233 PRIMER_LEFT_88_PENALTY=1.239865 PRIMER_RIGHT_88_PENALTY=1.405369 PRIMER_LEFT_88_SEQUENCE=GATTCCCTACCGCTGCTTAGT PRIMER_RIGHT_88_SEQUENCE=TTCGCAAACATCCATCCTC PRIMER_LEFT_88=535,21 PRIMER_RIGHT_88=634,19 PRIMER_LEFT_88_TM=59.760 PRIMER_RIGHT_88_TM=59.595 PRIMER_LEFT_88_GC_PERCENT=52.381 PRIMER_RIGHT_88_GC_PERCENT=47.368 PRIMER_LEFT_88_SELF_ANY=3.00 PRIMER_RIGHT_88_SELF_ANY=4.00 PRIMER_LEFT_88_SELF_END=1.00 PRIMER_RIGHT_88_SELF_END=0.00 PRIMER_LEFT_88_END_STABILITY=5.7000 PRIMER_RIGHT_88_END_STABILITY=7.9000 PRIMER_PAIR_88_COMPL_ANY=3.00 PRIMER_PAIR_88_COMPL_END=0.00 PRIMER_PAIR_88_PRODUCT_SIZE=100 PRIMER_PAIR_89_PENALTY=2.697526 PRIMER_LEFT_89_PENALTY=1.461462 PRIMER_RIGHT_89_PENALTY=1.236064 PRIMER_LEFT_89_SEQUENCE=TGGTAGAAGCCAACCAACCA PRIMER_RIGHT_89_SEQUENCE=CAAAGTGGGGATGGGTCTT PRIMER_LEFT_89=453,20 PRIMER_RIGHT_89=533,19 PRIMER_LEFT_89_TM=61.461 PRIMER_RIGHT_89_TM=59.764 PRIMER_LEFT_89_GC_PERCENT=50.000 PRIMER_RIGHT_89_GC_PERCENT=52.632 PRIMER_LEFT_89_SELF_ANY=4.00 PRIMER_RIGHT_89_SELF_ANY=3.00 PRIMER_LEFT_89_SELF_END=1.00 PRIMER_RIGHT_89_SELF_END=0.00 PRIMER_LEFT_89_END_STABILITY=8.2000 PRIMER_RIGHT_89_END_STABILITY=6.4000 PRIMER_PAIR_89_COMPL_ANY=4.00 PRIMER_PAIR_89_COMPL_END=3.00 PRIMER_PAIR_89_PRODUCT_SIZE=81 PRIMER_PAIR_90_PENALTY=2.715470 PRIMER_LEFT_90_PENALTY=1.043090 PRIMER_RIGHT_90_PENALTY=1.672380 PRIMER_LEFT_90_SEQUENCE=CTTCTCGTTCCTGACAAGTGC PRIMER_RIGHT_90_SEQUENCE=ATGTCTCTTTGGCGACGGT PRIMER_LEFT_90=578,21 PRIMER_RIGHT_90=671,19 PRIMER_LEFT_90_TM=60.043 PRIMER_RIGHT_90_TM=60.672 PRIMER_LEFT_90_GC_PERCENT=52.381 PRIMER_RIGHT_90_GC_PERCENT=52.632 PRIMER_LEFT_90_SELF_ANY=4.00 PRIMER_RIGHT_90_SELF_ANY=3.00 PRIMER_LEFT_90_SELF_END=2.00 PRIMER_RIGHT_90_SELF_END=3.00 PRIMER_LEFT_90_END_STABILITY=7.9000 PRIMER_RIGHT_90_END_STABILITY=9.3000 PRIMER_PAIR_90_COMPL_ANY=4.00 PRIMER_PAIR_90_COMPL_END=1.00 PRIMER_PAIR_90_PRODUCT_SIZE=94 PRIMER_PAIR_91_PENALTY=2.751265 PRIMER_LEFT_91_PENALTY=1.515201 PRIMER_RIGHT_91_PENALTY=1.236064 PRIMER_LEFT_91_SEQUENCE=AATGTGGTAGAAGCCAACCAA PRIMER_RIGHT_91_SEQUENCE=AGGGAATCACAAAGTGGGG PRIMER_LEFT_91=449,21 PRIMER_RIGHT_91=542,19 PRIMER_LEFT_91_TM=59.485 PRIMER_RIGHT_91_TM=59.764 PRIMER_LEFT_91_GC_PERCENT=42.857 PRIMER_RIGHT_91_GC_PERCENT=52.632 PRIMER_LEFT_91_SELF_ANY=4.00 PRIMER_RIGHT_91_SELF_ANY=3.00 PRIMER_LEFT_91_SELF_END=2.00 PRIMER_RIGHT_91_SELF_END=0.00 PRIMER_LEFT_91_END_STABILITY=8.2000 PRIMER_RIGHT_91_END_STABILITY=11.2000 PRIMER_PAIR_91_COMPL_ANY=4.00 PRIMER_PAIR_91_COMPL_END=1.00 PRIMER_PAIR_91_PRODUCT_SIZE=94 PRIMER_PAIR_92_PENALTY=2.755288 PRIMER_LEFT_92_PENALTY=1.159466 PRIMER_RIGHT_92_PENALTY=1.595822 PRIMER_LEFT_92_SEQUENCE=GAGAGGATGGATGTTTGCGA PRIMER_RIGHT_92_SEQUENCE=ACATGCCGTAGTCATGCAAGT PRIMER_LEFT_92=614,20 PRIMER_RIGHT_92=710,21 PRIMER_LEFT_92_TM=61.159 PRIMER_RIGHT_92_TM=60.596 PRIMER_LEFT_92_GC_PERCENT=50.000 PRIMER_RIGHT_92_GC_PERCENT=47.619 PRIMER_LEFT_92_SELF_ANY=2.00 PRIMER_RIGHT_92_SELF_ANY=6.00 PRIMER_LEFT_92_SELF_END=2.00 PRIMER_RIGHT_92_SELF_END=3.00 PRIMER_LEFT_92_END_STABILITY=10.2000 PRIMER_RIGHT_92_END_STABILITY=6.7000 PRIMER_PAIR_92_COMPL_ANY=4.00 PRIMER_PAIR_92_COMPL_END=1.00 PRIMER_PAIR_92_PRODUCT_SIZE=97 PRIMER_PAIR_93_PENALTY=2.766605 PRIMER_LEFT_93_PENALTY=1.526740 PRIMER_RIGHT_93_PENALTY=1.239865 PRIMER_LEFT_93_SEQUENCE=ACCATCCAGAACTGGTGCAA PRIMER_RIGHT_93_SEQUENCE=ACTAAGCAGCGGTAGGGAATC PRIMER_LEFT_93=476,20 PRIMER_RIGHT_93=555,21 PRIMER_LEFT_93_TM=61.527 PRIMER_RIGHT_93_TM=59.760 PRIMER_LEFT_93_GC_PERCENT=50.000 PRIMER_RIGHT_93_GC_PERCENT=52.381 PRIMER_LEFT_93_SELF_ANY=6.00 PRIMER_RIGHT_93_SELF_ANY=3.00 PRIMER_LEFT_93_SELF_END=2.00 PRIMER_RIGHT_93_SELF_END=3.00 PRIMER_LEFT_93_END_STABILITY=8.8000 PRIMER_RIGHT_93_END_STABILITY=6.6000 PRIMER_PAIR_93_COMPL_ANY=4.00 PRIMER_PAIR_93_COMPL_END=0.00 PRIMER_PAIR_93_PRODUCT_SIZE=80 PRIMER_PAIR_94_PENALTY=2.772752 PRIMER_LEFT_94_PENALTY=2.083070 PRIMER_RIGHT_94_PENALTY=0.689681 PRIMER_LEFT_94_SEQUENCE=CATTGATACCAAGGAAGGCAT PRIMER_RIGHT_94_SEQUENCE=TCACTGGTTGGTTGGCTTCT PRIMER_LEFT_94=385,21 PRIMER_RIGHT_94=476,20 PRIMER_LEFT_94_TM=58.917 PRIMER_RIGHT_94_TM=60.690 PRIMER_LEFT_94_GC_PERCENT=42.857 PRIMER_RIGHT_94_GC_PERCENT=50.000 PRIMER_LEFT_94_SELF_ANY=6.00 PRIMER_RIGHT_94_SELF_ANY=3.00 PRIMER_LEFT_94_SELF_END=2.00 PRIMER_RIGHT_94_SELF_END=0.00 PRIMER_LEFT_94_END_STABILITY=9.6000 PRIMER_RIGHT_94_END_STABILITY=6.7000 PRIMER_PAIR_94_COMPL_ANY=5.00 PRIMER_PAIR_94_COMPL_END=3.00 PRIMER_PAIR_94_PRODUCT_SIZE=92 PRIMER_PAIR_95_PENALTY=2.793905 PRIMER_LEFT_95_PENALTY=0.663548 PRIMER_RIGHT_95_PENALTY=2.130358 PRIMER_LEFT_95_SEQUENCE=AATGGGAAGTGGGATTCAGA PRIMER_RIGHT_95_SEQUENCE=TCAGGGTAGACTTCTTGGCAAT PRIMER_LEFT_95=344,20 PRIMER_RIGHT_95=435,22 PRIMER_LEFT_95_TM=59.336 PRIMER_RIGHT_95_TM=60.130 PRIMER_LEFT_95_GC_PERCENT=45.000 PRIMER_RIGHT_95_GC_PERCENT=45.455 PRIMER_LEFT_95_SELF_ANY=3.00 PRIMER_RIGHT_95_SELF_ANY=5.00 PRIMER_LEFT_95_SELF_END=3.00 PRIMER_RIGHT_95_SELF_END=3.00 PRIMER_LEFT_95_END_STABILITY=6.7000 PRIMER_RIGHT_95_END_STABILITY=8.4000 PRIMER_PAIR_95_COMPL_ANY=5.00 PRIMER_PAIR_95_COMPL_END=1.00 PRIMER_PAIR_95_PRODUCT_SIZE=92 PRIMER_PAIR_96_PENALTY=2.802419 PRIMER_LEFT_96_PENALTY=1.236064 PRIMER_RIGHT_96_PENALTY=1.566355 PRIMER_LEFT_96_SEQUENCE=CAAGACCCATCCCCACTTT PRIMER_RIGHT_96_SEQUENCE=GGAACGAGAAGGGCATCACT PRIMER_LEFT_96=514,19 PRIMER_RIGHT_96=588,20 PRIMER_LEFT_96_TM=59.764 PRIMER_RIGHT_96_TM=61.566 PRIMER_LEFT_96_GC_PERCENT=52.632 PRIMER_RIGHT_96_GC_PERCENT=55.000 PRIMER_LEFT_96_SELF_ANY=3.00 PRIMER_RIGHT_96_SELF_ANY=2.00 PRIMER_LEFT_96_SELF_END=0.00 PRIMER_RIGHT_96_SELF_END=2.00 PRIMER_LEFT_96_END_STABILITY=6.7000 PRIMER_RIGHT_96_END_STABILITY=6.4000 PRIMER_PAIR_96_COMPL_ANY=4.00 PRIMER_PAIR_96_COMPL_END=0.00 PRIMER_PAIR_96_PRODUCT_SIZE=75 PRIMER_PAIR_97_PENALTY=2.803473 PRIMER_LEFT_97_PENALTY=1.059537 PRIMER_RIGHT_97_PENALTY=1.743936 PRIMER_LEFT_97_SEQUENCE=CCAGGAGAGGATGGATGTTT PRIMER_RIGHT_97_SEQUENCE=ATGCCGTAGTCATGCAAGTT PRIMER_LEFT_97=610,20 PRIMER_RIGHT_97=708,20 PRIMER_LEFT_97_TM=58.940 PRIMER_RIGHT_97_TM=58.256 PRIMER_LEFT_97_GC_PERCENT=50.000 PRIMER_RIGHT_97_GC_PERCENT=45.000 PRIMER_LEFT_97_SELF_ANY=3.00 PRIMER_RIGHT_97_SELF_ANY=4.00 PRIMER_LEFT_97_SELF_END=0.00 PRIMER_RIGHT_97_SELF_END=3.00 PRIMER_LEFT_97_END_STABILITY=7.0000 PRIMER_RIGHT_97_END_STABILITY=6.7000 PRIMER_PAIR_97_COMPL_ANY=4.00 PRIMER_PAIR_97_COMPL_END=1.00 PRIMER_PAIR_97_PRODUCT_SIZE=99 PRIMER_PAIR_98_PENALTY=2.807694 PRIMER_LEFT_98_PENALTY=0.807658 PRIMER_RIGHT_98_PENALTY=2.000037 PRIMER_LEFT_98_SEQUENCE=GATCCATCAGGGACCAAAAC PRIMER_RIGHT_98_SEQUENCE=TTCTACCACATTGGTGATCTGC PRIMER_LEFT_98=362,20 PRIMER_RIGHT_98=460,22 PRIMER_LEFT_98_TM=59.192 PRIMER_RIGHT_98_TM=60.000 PRIMER_LEFT_98_GC_PERCENT=50.000 PRIMER_RIGHT_98_GC_PERCENT=45.455 PRIMER_LEFT_98_SELF_ANY=5.00 PRIMER_RIGHT_98_SELF_ANY=6.00 PRIMER_LEFT_98_SELF_END=0.00 PRIMER_RIGHT_98_SELF_END=2.00 PRIMER_LEFT_98_END_STABILITY=7.0000 PRIMER_RIGHT_98_END_STABILITY=8.2000 PRIMER_PAIR_98_COMPL_ANY=5.00 PRIMER_PAIR_98_COMPL_END=1.00 PRIMER_PAIR_98_PRODUCT_SIZE=99 PRIMER_PAIR_99_PENALTY=2.808409 PRIMER_LEFT_99_PENALTY=1.251196 PRIMER_RIGHT_99_PENALTY=1.557214 PRIMER_LEFT_99_SEQUENCE=TGTAAGTGATGCCCTTCTCGT PRIMER_RIGHT_99_SEQUENCE=CCAGTGAAGATGAGTTTCGC PRIMER_LEFT_99=565,21 PRIMER_RIGHT_99=649,20 PRIMER_LEFT_99_TM=59.749 PRIMER_RIGHT_99_TM=58.443 PRIMER_LEFT_99_GC_PERCENT=47.619 PRIMER_RIGHT_99_GC_PERCENT=50.000 PRIMER_LEFT_99_SELF_ANY=4.00 PRIMER_RIGHT_99_SELF_ANY=3.00 PRIMER_LEFT_99_SELF_END=2.00 PRIMER_RIGHT_99_SELF_END=3.00 PRIMER_LEFT_99_END_STABILITY=8.1000 PRIMER_RIGHT_99_END_STABILITY=10.2000 PRIMER_PAIR_99_COMPL_ANY=4.00 PRIMER_PAIR_99_COMPL_END=1.00 PRIMER_PAIR_99_PRODUCT_SIZE=85 PRIMER_PAIR_100_PENALTY=2.812889 PRIMER_LEFT_100_PENALTY=1.255675 PRIMER_RIGHT_100_PENALTY=1.557214 PRIMER_LEFT_100_SEQUENCE=TTGTAAGTGATGCCCTTCTCG PRIMER_RIGHT_100_SEQUENCE=GCCAGTGAAGATGAGTTTCG PRIMER_LEFT_100=564,21 PRIMER_RIGHT_100=650,20 PRIMER_LEFT_100_TM=60.256 PRIMER_RIGHT_100_TM=58.443 PRIMER_LEFT_100_GC_PERCENT=47.619 PRIMER_RIGHT_100_GC_PERCENT=50.000 PRIMER_LEFT_100_SELF_ANY=4.00 PRIMER_RIGHT_100_SELF_ANY=3.00 PRIMER_LEFT_100_SELF_END=2.00 PRIMER_RIGHT_100_SELF_END=2.00 PRIMER_LEFT_100_END_STABILITY=8.4000 PRIMER_RIGHT_100_END_STABILITY=9.0000 PRIMER_PAIR_100_COMPL_ANY=4.00 PRIMER_PAIR_100_COMPL_END=2.00 PRIMER_PAIR_100_PRODUCT_SIZE=87 PRIMER_PAIR_101_PENALTY=2.835338 PRIMER_LEFT_101_PENALTY=1.557214 PRIMER_RIGHT_101_PENALTY=1.278124 PRIMER_LEFT_101_SEQUENCE=ACCCACTGATGGTAATGCTG PRIMER_RIGHT_101_SEQUENCE=ATCCCACTTCCCATTCTGG PRIMER_LEFT_101=259,20 PRIMER_RIGHT_101=358,19 PRIMER_LEFT_101_TM=58.443 PRIMER_RIGHT_101_TM=59.722 PRIMER_LEFT_101_GC_PERCENT=50.000 PRIMER_RIGHT_101_GC_PERCENT=52.632 PRIMER_LEFT_101_SELF_ANY=3.00 PRIMER_RIGHT_101_SELF_ANY=3.00 PRIMER_LEFT_101_SELF_END=1.00 PRIMER_RIGHT_101_SELF_END=3.00 PRIMER_LEFT_101_END_STABILITY=8.5000 PRIMER_RIGHT_101_END_STABILITY=8.2000 PRIMER_PAIR_101_COMPL_ANY=5.00 PRIMER_PAIR_101_COMPL_END=3.00 PRIMER_PAIR_101_PRODUCT_SIZE=100 PRIMER_PAIR_102_PENALTY=2.840973 PRIMER_LEFT_102_PENALTY=1.977994 PRIMER_RIGHT_102_PENALTY=0.862979 PRIMER_LEFT_102_SEQUENCE=CCAAAGAGACATGCAGTGAGA PRIMER_RIGHT_102_SEQUENCE=TCTACCCCTCGGAACTTGTC PRIMER_LEFT_102=660,21 PRIMER_RIGHT_102=747,20 PRIMER_LEFT_102_TM=59.022 PRIMER_RIGHT_102_TM=59.137 PRIMER_LEFT_102_GC_PERCENT=47.619 PRIMER_RIGHT_102_GC_PERCENT=55.000 PRIMER_LEFT_102_SELF_ANY=4.00 PRIMER_RIGHT_102_SELF_ANY=3.00 PRIMER_LEFT_102_SELF_END=0.00 PRIMER_RIGHT_102_SELF_END=1.00 PRIMER_LEFT_102_END_STABILITY=6.7000 PRIMER_RIGHT_102_END_STABILITY=6.7000 PRIMER_PAIR_102_COMPL_ANY=4.00 PRIMER_PAIR_102_COMPL_END=2.00 PRIMER_PAIR_102_PRODUCT_SIZE=88 PRIMER_PAIR_103_PENALTY=2.919658 PRIMER_LEFT_103_PENALTY=1.236064 PRIMER_RIGHT_103_PENALTY=1.683594 PRIMER_LEFT_103_SEQUENCE=CCCCACTTTGTGATTCCCT PRIMER_RIGHT_103_SEQUENCE=ATTTGCACTTGTCAGGAACGA PRIMER_LEFT_103=524,19 PRIMER_RIGHT_103=602,21 PRIMER_LEFT_103_TM=59.764 PRIMER_RIGHT_103_TM=60.684 PRIMER_LEFT_103_GC_PERCENT=52.632 PRIMER_RIGHT_103_GC_PERCENT=42.857 PRIMER_LEFT_103_SELF_ANY=3.00 PRIMER_RIGHT_103_SELF_ANY=4.00 PRIMER_LEFT_103_SELF_END=1.00 PRIMER_RIGHT_103_SELF_END=1.00 PRIMER_LEFT_103_END_STABILITY=9.4000 PRIMER_RIGHT_103_END_STABILITY=8.4000 PRIMER_PAIR_103_COMPL_ANY=4.00 PRIMER_PAIR_103_COMPL_END=2.00 PRIMER_PAIR_103_PRODUCT_SIZE=79 PRIMER_PAIR_104_PENALTY=2.929327 PRIMER_LEFT_104_PENALTY=1.991012 PRIMER_RIGHT_104_PENALTY=0.938316 PRIMER_LEFT_104_SEQUENCE=GCCAAAGAGACATGCAGTGAG PRIMER_RIGHT_104_SEQUENCE=ACACAAACTCTACCCCTCGG PRIMER_LEFT_104=659,21 PRIMER_RIGHT_104=755,20 PRIMER_LEFT_104_TM=60.991 PRIMER_RIGHT_104_TM=59.062 PRIMER_LEFT_104_GC_PERCENT=52.381 PRIMER_RIGHT_104_GC_PERCENT=55.000 PRIMER_LEFT_104_SELF_ANY=4.00 PRIMER_RIGHT_104_SELF_ANY=2.00 PRIMER_LEFT_104_SELF_END=0.00 PRIMER_RIGHT_104_SELF_END=2.00 PRIMER_LEFT_104_END_STABILITY=6.4000 PRIMER_RIGHT_104_END_STABILITY=9.9000 PRIMER_PAIR_104_COMPL_ANY=4.00 PRIMER_PAIR_104_COMPL_END=1.00 PRIMER_PAIR_104_PRODUCT_SIZE=97 PRIMER_PAIR_105_PENALTY=2.936620 PRIMER_LEFT_105_PENALTY=1.810378 PRIMER_RIGHT_105_PENALTY=1.126243 PRIMER_LEFT_105_SEQUENCE=CAAGCAGTGCAAGACCCATC PRIMER_RIGHT_105_SEQUENCE=CGAGAAGGGCATCACTTACA PRIMER_LEFT_105=505,20 PRIMER_RIGHT_105=584,20 PRIMER_LEFT_105_TM=61.810 PRIMER_RIGHT_105_TM=58.874 PRIMER_LEFT_105_GC_PERCENT=55.000 PRIMER_RIGHT_105_GC_PERCENT=50.000 PRIMER_LEFT_105_SELF_ANY=5.00 PRIMER_RIGHT_105_SELF_ANY=4.00 PRIMER_LEFT_105_SELF_END=0.00 PRIMER_RIGHT_105_SELF_END=0.00 PRIMER_LEFT_105_END_STABILITY=8.1000 PRIMER_RIGHT_105_END_STABILITY=6.0000 PRIMER_PAIR_105_COMPL_ANY=5.00 PRIMER_PAIR_105_COMPL_END=2.00 PRIMER_PAIR_105_PRODUCT_SIZE=80 PRIMER_PAIR_106_PENALTY=2.957123 PRIMER_LEFT_106_PENALTY=1.342473 PRIMER_RIGHT_106_PENALTY=1.614650 PRIMER_LEFT_106_SEQUENCE=TTGTGATTCCCTACCGCTG PRIMER_RIGHT_106_SEQUENCE=AACATCCATCCTCTCCTGGT PRIMER_LEFT_106=531,19 PRIMER_RIGHT_106=628,20 PRIMER_LEFT_106_TM=59.658 PRIMER_RIGHT_106_TM=58.385 PRIMER_LEFT_106_GC_PERCENT=52.632 PRIMER_RIGHT_106_GC_PERCENT=50.000 PRIMER_LEFT_106_SELF_ANY=3.00 PRIMER_RIGHT_106_SELF_ANY=3.00 PRIMER_LEFT_106_SELF_END=2.00 PRIMER_RIGHT_106_SELF_END=1.00 PRIMER_LEFT_106_END_STABILITY=10.2000 PRIMER_RIGHT_106_END_STABILITY=7.9000 PRIMER_PAIR_106_COMPL_ANY=3.00 PRIMER_PAIR_106_COMPL_END=1.00 PRIMER_PAIR_106_PRODUCT_SIZE=98 PRIMER_PAIR_107_PENALTY=2.960724 PRIMER_LEFT_107_PENALTY=1.532916 PRIMER_RIGHT_107_PENALTY=1.427808 PRIMER_LEFT_107_SEQUENCE=CATGCACATGAATGTCCAGAA PRIMER_RIGHT_107_SEQUENCE=TTCCTTGGTATCAATGCAGG PRIMER_LEFT_107=325,21 PRIMER_RIGHT_107=400,20 PRIMER_LEFT_107_TM=60.533 PRIMER_RIGHT_107_TM=58.572 PRIMER_LEFT_107_GC_PERCENT=42.857 PRIMER_RIGHT_107_GC_PERCENT=45.000 PRIMER_LEFT_107_SELF_ANY=8.00 PRIMER_RIGHT_107_SELF_ANY=6.00 PRIMER_LEFT_107_SELF_END=3.00 PRIMER_RIGHT_107_SELF_END=2.00 PRIMER_LEFT_107_END_STABILITY=7.0000 PRIMER_RIGHT_107_END_STABILITY=9.7000 PRIMER_PAIR_107_COMPL_ANY=4.00 PRIMER_PAIR_107_COMPL_END=3.00 PRIMER_PAIR_107_PRODUCT_SIZE=76 PRIMER_PAIR_108_PENALTY=2.979016 PRIMER_LEFT_108_PENALTY=2.315468 PRIMER_RIGHT_108_PENALTY=0.663548 PRIMER_LEFT_108_SEQUENCE=CTGATGGTAATGCTGGCCT PRIMER_RIGHT_108_SEQUENCE=TGAATCCCACTTCCCATTCT PRIMER_LEFT_108=264,19 PRIMER_RIGHT_108=361,20 PRIMER_LEFT_108_TM=58.685 PRIMER_RIGHT_108_TM=59.336 PRIMER_LEFT_108_GC_PERCENT=52.632 PRIMER_RIGHT_108_GC_PERCENT=45.000 PRIMER_LEFT_108_SELF_ANY=4.00 PRIMER_RIGHT_108_SELF_ANY=4.00 PRIMER_LEFT_108_SELF_END=2.00 PRIMER_RIGHT_108_SELF_END=0.00 PRIMER_LEFT_108_END_STABILITY=10.9000 PRIMER_RIGHT_108_END_STABILITY=6.6000 PRIMER_PAIR_108_COMPL_ANY=5.00 PRIMER_PAIR_108_COMPL_END=2.00 PRIMER_PAIR_108_PRODUCT_SIZE=98 PRIMER_PAIR_109_PENALTY=2.990855 PRIMER_LEFT_109_PENALTY=1.107745 PRIMER_RIGHT_109_PENALTY=1.883110 PRIMER_LEFT_109_SEQUENCE=CCTTCTCGTTCCTGACAAGTG PRIMER_RIGHT_109_SEQUENCE=ACGGTGTGCCAGTGAAGAT PRIMER_LEFT_109=577,21 PRIMER_RIGHT_109=657,19 PRIMER_LEFT_109_TM=59.892 PRIMER_RIGHT_109_TM=59.117 PRIMER_LEFT_109_GC_PERCENT=52.381 PRIMER_RIGHT_109_GC_PERCENT=52.632 PRIMER_LEFT_109_SELF_ANY=4.00 PRIMER_RIGHT_109_SELF_ANY=3.00 PRIMER_LEFT_109_SELF_END=2.00 PRIMER_RIGHT_109_SELF_END=2.00 PRIMER_LEFT_109_END_STABILITY=6.7000 PRIMER_RIGHT_109_END_STABILITY=6.6000 PRIMER_PAIR_109_COMPL_ANY=5.00 PRIMER_PAIR_109_COMPL_END=2.00 PRIMER_PAIR_109_PRODUCT_SIZE=81 PRIMER_PAIR_110_PENALTY=3.016344 PRIMER_LEFT_110_PENALTY=1.673871 PRIMER_RIGHT_110_PENALTY=1.342473 PRIMER_LEFT_110_SEQUENCE=TAGAAGCCAACCAACCAGTGA PRIMER_RIGHT_110_SEQUENCE=CAGCGGTAGGGAATCACAA PRIMER_LEFT_110=456,21 PRIMER_RIGHT_110=549,19 PRIMER_LEFT_110_TM=60.674 PRIMER_RIGHT_110_TM=59.658 PRIMER_LEFT_110_GC_PERCENT=47.619 PRIMER_RIGHT_110_GC_PERCENT=52.632 PRIMER_LEFT_110_SELF_ANY=3.00 PRIMER_RIGHT_110_SELF_ANY=3.00 PRIMER_LEFT_110_SELF_END=1.00 PRIMER_RIGHT_110_SELF_END=3.00 PRIMER_LEFT_110_END_STABILITY=6.4000 PRIMER_RIGHT_110_END_STABILITY=7.0000 PRIMER_PAIR_110_COMPL_ANY=4.00 PRIMER_PAIR_110_COMPL_END=2.00 PRIMER_PAIR_110_PRODUCT_SIZE=94 PRIMER_PAIR_111_PENALTY=3.023707 PRIMER_LEFT_111_PENALTY=1.876405 PRIMER_RIGHT_111_PENALTY=1.147302 PRIMER_LEFT_111_SEQUENCE=CCCCAGATTGCCATGTTCT PRIMER_RIGHT_111_SEQUENCE=ATGGATCTGAATCCCACTTCC PRIMER_LEFT_111=293,19 PRIMER_RIGHT_111=368,21 PRIMER_LEFT_111_TM=60.876 PRIMER_RIGHT_111_TM=60.147 PRIMER_LEFT_111_GC_PERCENT=52.632 PRIMER_RIGHT_111_GC_PERCENT=47.619 PRIMER_LEFT_111_SELF_ANY=4.00 PRIMER_RIGHT_111_SELF_ANY=5.00 PRIMER_LEFT_111_SELF_END=0.00 PRIMER_RIGHT_111_SELF_END=2.00 PRIMER_LEFT_111_END_STABILITY=6.4000 PRIMER_RIGHT_111_END_STABILITY=8.2000 PRIMER_PAIR_111_COMPL_ANY=5.00 PRIMER_PAIR_111_COMPL_END=0.00 PRIMER_PAIR_111_PRODUCT_SIZE=76 PRIMER_PAIR_112_PENALTY=3.028872 PRIMER_LEFT_112_PENALTY=1.513670 PRIMER_RIGHT_112_PENALTY=1.515201 PRIMER_LEFT_112_SEQUENCE=GGACCAAAACCTGCATTGA PRIMER_RIGHT_112_SEQUENCE=TTGGTTGGCTTCTACCACATT PRIMER_LEFT_112=372,19 PRIMER_RIGHT_112=469,21 PRIMER_LEFT_112_TM=59.486 PRIMER_RIGHT_112_TM=59.485 PRIMER_LEFT_112_GC_PERCENT=47.368 PRIMER_RIGHT_112_GC_PERCENT=42.857 PRIMER_LEFT_112_SELF_ANY=4.00 PRIMER_RIGHT_112_SELF_ANY=4.00 PRIMER_LEFT_112_SELF_END=1.00 PRIMER_RIGHT_112_SELF_END=1.00 PRIMER_LEFT_112_END_STABILITY=6.9000 PRIMER_RIGHT_112_END_STABILITY=6.6000 PRIMER_PAIR_112_COMPL_ANY=5.00 PRIMER_PAIR_112_COMPL_END=1.00 PRIMER_PAIR_112_PRODUCT_SIZE=98 PRIMER_PAIR_113_PENALTY=3.039862 PRIMER_LEFT_113_PENALTY=2.376315 PRIMER_RIGHT_113_PENALTY=0.663548 PRIMER_LEFT_113_SEQUENCE=GGTAATGCTGGCCTGCTG PRIMER_RIGHT_113_SEQUENCE=CTGAATCCCACTTCCCATTC PRIMER_LEFT_113=269,18 PRIMER_RIGHT_113=362,20 PRIMER_LEFT_113_TM=60.376 PRIMER_RIGHT_113_TM=59.336 PRIMER_LEFT_113_GC_PERCENT=61.111 PRIMER_RIGHT_113_GC_PERCENT=50.000 PRIMER_LEFT_113_SELF_ANY=6.00 PRIMER_RIGHT_113_SELF_ANY=4.00 PRIMER_LEFT_113_SELF_END=2.00 PRIMER_RIGHT_113_SELF_END=2.00 PRIMER_LEFT_113_END_STABILITY=8.5000 PRIMER_RIGHT_113_END_STABILITY=6.9000 PRIMER_PAIR_113_COMPL_ANY=4.00 PRIMER_PAIR_113_COMPL_END=2.00 PRIMER_PAIR_113_PRODUCT_SIZE=94 PRIMER_PAIR_114_PENALTY=3.050373 PRIMER_LEFT_114_PENALTY=1.654904 PRIMER_RIGHT_114_PENALTY=1.395469 PRIMER_LEFT_114_SEQUENCE=CCATCCAGAACTGGTGCAAG PRIMER_RIGHT_114_SEQUENCE=CACCAACTAAGCAGCGGTAG PRIMER_LEFT_114=477,20 PRIMER_RIGHT_114=560,20 PRIMER_LEFT_114_TM=61.655 PRIMER_RIGHT_114_TM=58.605 PRIMER_LEFT_114_GC_PERCENT=55.000 PRIMER_RIGHT_114_GC_PERCENT=55.000 PRIMER_LEFT_114_SELF_ANY=6.00 PRIMER_RIGHT_114_SELF_ANY=3.00 PRIMER_LEFT_114_SELF_END=2.00 PRIMER_RIGHT_114_SELF_END=3.00 PRIMER_LEFT_114_END_STABILITY=8.5000 PRIMER_RIGHT_114_END_STABILITY=6.9000 PRIMER_PAIR_114_COMPL_ANY=5.00 PRIMER_PAIR_114_COMPL_END=1.00 PRIMER_PAIR_114_PRODUCT_SIZE=84 PRIMER_PAIR_115_PENALTY=3.070713 PRIMER_LEFT_115_PENALTY=1.670728 PRIMER_RIGHT_115_PENALTY=1.399985 PRIMER_LEFT_115_SEQUENCE=CCATCCAGAACTGGTGCAA PRIMER_RIGHT_115_SEQUENCE=CTCACCAACTAAGCAGCGG PRIMER_LEFT_115=477,19 PRIMER_RIGHT_115=562,19 PRIMER_LEFT_115_TM=60.671 PRIMER_RIGHT_115_TM=59.600 PRIMER_LEFT_115_GC_PERCENT=52.632 PRIMER_RIGHT_115_GC_PERCENT=57.895 PRIMER_LEFT_115_SELF_ANY=6.00 PRIMER_RIGHT_115_SELF_ANY=3.00 PRIMER_LEFT_115_SELF_END=2.00 PRIMER_RIGHT_115_SELF_END=2.00 PRIMER_LEFT_115_END_STABILITY=8.8000 PRIMER_RIGHT_115_END_STABILITY=11.4000 PRIMER_PAIR_115_COMPL_ANY=5.00 PRIMER_PAIR_115_COMPL_END=1.00 PRIMER_PAIR_115_PRODUCT_SIZE=86 PRIMER_PAIR_116_PENALTY=3.099513 PRIMER_LEFT_116_PENALTY=1.659162 PRIMER_RIGHT_116_PENALTY=1.440351 PRIMER_LEFT_116_SEQUENCE=CATGTTCTGTGGCAGACTGA PRIMER_RIGHT_116_SEQUENCE=TTGGTCCCTGATGGATCTGA PRIMER_LEFT_116=304,20 PRIMER_RIGHT_116=378,20 PRIMER_LEFT_116_TM=58.341 PRIMER_RIGHT_116_TM=61.440 PRIMER_LEFT_116_GC_PERCENT=50.000 PRIMER_RIGHT_116_GC_PERCENT=50.000 PRIMER_LEFT_116_SELF_ANY=5.00 PRIMER_RIGHT_116_SELF_ANY=5.00 PRIMER_LEFT_116_SELF_END=3.00 PRIMER_RIGHT_116_SELF_END=3.00 PRIMER_LEFT_116_END_STABILITY=6.4000 PRIMER_RIGHT_116_END_STABILITY=6.7000 PRIMER_PAIR_116_COMPL_ANY=4.00 PRIMER_PAIR_116_COMPL_END=1.00 PRIMER_PAIR_116_PRODUCT_SIZE=75 PRIMER_PAIR_117_PENALTY=3.108139 PRIMER_LEFT_117_PENALTY=2.047791 PRIMER_RIGHT_117_PENALTY=1.060348 PRIMER_LEFT_117_SEQUENCE=TTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_117_SEQUENCE=CACCAGTTCTGGATGGTCAC PRIMER_LEFT_117=415,20 PRIMER_RIGHT_117=492,20 PRIMER_LEFT_117_TM=57.952 PRIMER_RIGHT_117_TM=58.940 PRIMER_LEFT_117_GC_PERCENT=50.000 PRIMER_RIGHT_117_GC_PERCENT=55.000 PRIMER_LEFT_117_SELF_ANY=5.00 PRIMER_RIGHT_117_SELF_ANY=6.00 PRIMER_LEFT_117_SELF_END=1.00 PRIMER_RIGHT_117_SELF_END=3.00 PRIMER_LEFT_117_END_STABILITY=9.7000 PRIMER_RIGHT_117_END_STABILITY=6.1000 PRIMER_PAIR_117_COMPL_ANY=4.00 PRIMER_PAIR_117_COMPL_END=1.00 PRIMER_PAIR_117_PRODUCT_SIZE=78 PRIMER_PAIR_118_PENALTY=3.121455 PRIMER_LEFT_118_PENALTY=1.188029 PRIMER_RIGHT_118_PENALTY=1.933425 PRIMER_LEFT_118_SEQUENCE=ACATGAATGTCCAGAATGGGA PRIMER_RIGHT_118_SEQUENCE=AGGATGCCTTCCTTGGTATC PRIMER_LEFT_118=330,21 PRIMER_RIGHT_118=408,20 PRIMER_LEFT_118_TM=60.188 PRIMER_RIGHT_118_TM=58.067 PRIMER_LEFT_118_GC_PERCENT=42.857 PRIMER_RIGHT_118_GC_PERCENT=50.000 PRIMER_LEFT_118_SELF_ANY=6.00 PRIMER_RIGHT_118_SELF_ANY=7.00 PRIMER_LEFT_118_SELF_END=1.00 PRIMER_RIGHT_118_SELF_END=2.00 PRIMER_LEFT_118_END_STABILITY=9.7000 PRIMER_RIGHT_118_END_STABILITY=5.3000 PRIMER_PAIR_118_COMPL_ANY=3.00 PRIMER_PAIR_118_COMPL_END=2.00 PRIMER_PAIR_118_PRODUCT_SIZE=79 PRIMER_PAIR_119_PENALTY=3.124455 PRIMER_LEFT_119_PENALTY=1.224915 PRIMER_RIGHT_119_PENALTY=1.899540 PRIMER_LEFT_119_SEQUENCE=CGTTCCTGACAAGTGCAAAT PRIMER_RIGHT_119_SEQUENCE=GACGGTGTGCCAGTGAAGA PRIMER_LEFT_119=583,20 PRIMER_RIGHT_119=658,19 PRIMER_LEFT_119_TM=58.775 PRIMER_RIGHT_119_TM=60.900 PRIMER_LEFT_119_GC_PERCENT=45.000 PRIMER_RIGHT_119_GC_PERCENT=57.895 PRIMER_LEFT_119_SELF_ANY=4.00 PRIMER_RIGHT_119_SELF_ANY=3.00 PRIMER_LEFT_119_SELF_END=2.00 PRIMER_RIGHT_119_SELF_END=0.00 PRIMER_LEFT_119_END_STABILITY=7.2000 PRIMER_RIGHT_119_END_STABILITY=6.7000 PRIMER_PAIR_119_COMPL_ANY=5.00 PRIMER_PAIR_119_COMPL_END=1.00 PRIMER_PAIR_119_PRODUCT_SIZE=76 PRIMER_PAIR_120_PENALTY=3.126172 PRIMER_LEFT_120_PENALTY=1.546028 PRIMER_RIGHT_120_PENALTY=1.580144 PRIMER_LEFT_120_SEQUENCE=TGTTTGCGAAACTCATCTTCA PRIMER_RIGHT_120_SEQUENCE=CAGCAACATGCCGTAGTCA PRIMER_LEFT_120=625,21 PRIMER_RIGHT_120=715,19 PRIMER_LEFT_120_TM=59.454 PRIMER_RIGHT_120_TM=59.420 PRIMER_LEFT_120_GC_PERCENT=38.095 PRIMER_RIGHT_120_GC_PERCENT=52.632 PRIMER_LEFT_120_SELF_ANY=6.00 PRIMER_RIGHT_120_SELF_ANY=4.00 PRIMER_LEFT_120_SELF_END=1.00 PRIMER_RIGHT_120_SELF_END=2.00 PRIMER_LEFT_120_END_STABILITY=7.0000 PRIMER_RIGHT_120_END_STABILITY=6.4000 PRIMER_PAIR_120_COMPL_ANY=4.00 PRIMER_PAIR_120_COMPL_END=1.00 PRIMER_PAIR_120_PRODUCT_SIZE=91 PRIMER_PAIR_121_PENALTY=3.144101 PRIMER_LEFT_121_PENALTY=1.630431 PRIMER_RIGHT_121_PENALTY=1.513670 PRIMER_LEFT_121_SEQUENCE=TGGCAGACTGAACATGCAC PRIMER_RIGHT_121_SEQUENCE=TCAATGCAGGTTTTGGTCC PRIMER_LEFT_121=313,19 PRIMER_RIGHT_121=390,19 PRIMER_LEFT_121_TM=59.370 PRIMER_RIGHT_121_TM=59.486 PRIMER_LEFT_121_GC_PERCENT=52.632 PRIMER_RIGHT_121_GC_PERCENT=47.368 PRIMER_LEFT_121_SELF_ANY=5.00 PRIMER_RIGHT_121_SELF_ANY=4.00 PRIMER_LEFT_121_SELF_END=2.00 PRIMER_RIGHT_121_SELF_END=3.00 PRIMER_LEFT_121_END_STABILITY=8.2000 PRIMER_RIGHT_121_END_STABILITY=9.1000 PRIMER_PAIR_121_COMPL_ANY=6.00 PRIMER_PAIR_121_COMPL_END=3.00 PRIMER_PAIR_121_PRODUCT_SIZE=78 PRIMER_PAIR_122_PENALTY=3.180910 PRIMER_LEFT_122_PENALTY=1.395469 PRIMER_RIGHT_122_PENALTY=1.785441 PRIMER_LEFT_122_SEQUENCE=CTACCGCTGCTTAGTTGGTG PRIMER_RIGHT_122_SEQUENCE=ACATCCATCCTCTCCTGGTGT PRIMER_LEFT_122=541,20 PRIMER_RIGHT_122=627,21 PRIMER_LEFT_122_TM=58.605 PRIMER_RIGHT_122_TM=60.785 PRIMER_LEFT_122_GC_PERCENT=55.000 PRIMER_RIGHT_122_GC_PERCENT=52.381 PRIMER_LEFT_122_SELF_ANY=3.00 PRIMER_RIGHT_122_SELF_ANY=4.00 PRIMER_LEFT_122_SELF_END=0.00 PRIMER_RIGHT_122_SELF_END=1.00 PRIMER_LEFT_122_END_STABILITY=8.2000 PRIMER_RIGHT_122_END_STABILITY=7.6000 PRIMER_PAIR_122_COMPL_ANY=3.00 PRIMER_PAIR_122_COMPL_END=0.00 PRIMER_PAIR_122_PRODUCT_SIZE=87 PRIMER_PAIR_123_PENALTY=3.191646 PRIMER_LEFT_123_PENALTY=1.781455 PRIMER_RIGHT_123_PENALTY=1.410191 PRIMER_LEFT_123_SEQUENCE=CCAACCAACCAGTGACCAT PRIMER_RIGHT_123_SEQUENCE=ATCACAAAGTGGGGATGGGT PRIMER_LEFT_123=462,19 PRIMER_RIGHT_123=537,20 PRIMER_LEFT_123_TM=59.219 PRIMER_RIGHT_123_TM=61.410 PRIMER_LEFT_123_GC_PERCENT=52.632 PRIMER_RIGHT_123_GC_PERCENT=50.000 PRIMER_LEFT_123_SELF_ANY=3.00 PRIMER_RIGHT_123_SELF_ANY=4.00 PRIMER_LEFT_123_SELF_END=2.00 PRIMER_RIGHT_123_SELF_END=0.00 PRIMER_LEFT_123_END_STABILITY=7.8000 PRIMER_RIGHT_123_END_STABILITY=9.4000 PRIMER_PAIR_123_COMPL_ANY=4.00 PRIMER_PAIR_123_COMPL_END=3.00 PRIMER_PAIR_123_PRODUCT_SIZE=76 PRIMER_PAIR_124_PENALTY=3.198555 PRIMER_LEFT_124_PENALTY=2.051254 PRIMER_RIGHT_124_PENALTY=1.147302 PRIMER_LEFT_124_SEQUENCE=AACCCCAGATTGCCATGTTC PRIMER_RIGHT_124_SEQUENCE=GATCTGAATCCCACTTCCCAT PRIMER_LEFT_124=291,20 PRIMER_RIGHT_124=365,21 PRIMER_LEFT_124_TM=62.051 PRIMER_RIGHT_124_TM=60.147 PRIMER_LEFT_124_GC_PERCENT=50.000 PRIMER_RIGHT_124_GC_PERCENT=47.619 PRIMER_LEFT_124_SELF_ANY=4.00 PRIMER_RIGHT_124_SELF_ANY=4.00 PRIMER_LEFT_124_SELF_END=0.00 PRIMER_RIGHT_124_SELF_END=2.00 PRIMER_LEFT_124_END_STABILITY=6.7000 PRIMER_RIGHT_124_END_STABILITY=9.6000 PRIMER_PAIR_124_COMPL_ANY=5.00 PRIMER_PAIR_124_COMPL_END=0.00 PRIMER_PAIR_124_PRODUCT_SIZE=75 PRIMER_PAIR_125_PENALTY=3.212911 PRIMER_LEFT_125_PENALTY=2.124336 PRIMER_RIGHT_125_PENALTY=1.088575 PRIMER_LEFT_125_SEQUENCE=TCGCCAAAGAGACATGCAG PRIMER_RIGHT_125_SEQUENCE=CCCCTCGGAACTTGTCAAT PRIMER_LEFT_125=657,19 PRIMER_RIGHT_125=743,19 PRIMER_LEFT_125_TM=61.124 PRIMER_RIGHT_125_TM=59.911 PRIMER_LEFT_125_GC_PERCENT=52.632 PRIMER_RIGHT_125_GC_PERCENT=52.632 PRIMER_LEFT_125_SELF_ANY=4.00 PRIMER_RIGHT_125_SELF_ANY=5.00 PRIMER_LEFT_125_SELF_END=2.00 PRIMER_RIGHT_125_SELF_END=3.00 PRIMER_LEFT_125_END_STABILITY=8.5000 PRIMER_RIGHT_125_END_STABILITY=6.9000 PRIMER_PAIR_125_COMPL_ANY=4.00 PRIMER_PAIR_125_COMPL_END=0.00 PRIMER_PAIR_125_PRODUCT_SIZE=87 PRIMER_PAIR_126_PENALTY=3.218494 PRIMER_LEFT_126_PENALTY=1.399985 PRIMER_RIGHT_126_PENALTY=1.818509 PRIMER_LEFT_126_SEQUENCE=CCGCTGCTTAGTTGGTGAG PRIMER_RIGHT_126_SEQUENCE=CGCAAACATCCATCCTCTC PRIMER_LEFT_126=544,19 PRIMER_RIGHT_126=632,19 PRIMER_LEFT_126_TM=59.600 PRIMER_RIGHT_126_TM=59.181 PRIMER_LEFT_126_GC_PERCENT=57.895 PRIMER_RIGHT_126_GC_PERCENT=52.632 PRIMER_LEFT_126_SELF_ANY=3.00 PRIMER_RIGHT_126_SELF_ANY=2.00 PRIMER_LEFT_126_SELF_END=0.00 PRIMER_RIGHT_126_SELF_END=0.00 PRIMER_LEFT_126_END_STABILITY=6.4000 PRIMER_RIGHT_126_END_STABILITY=6.4000 PRIMER_PAIR_126_COMPL_ANY=3.00 PRIMER_PAIR_126_COMPL_END=3.00 PRIMER_PAIR_126_PRODUCT_SIZE=89 PRIMER_PAIR_127_PENALTY=3.234917 PRIMER_LEFT_127_PENALTY=1.630431 PRIMER_RIGHT_127_PENALTY=1.604487 PRIMER_LEFT_127_SEQUENCE=TGTGGCAGACTGAACATGC PRIMER_RIGHT_127_SEQUENCE=AATGCAGGTTTTGGTCCCT PRIMER_LEFT_127=311,19 PRIMER_RIGHT_127=388,19 PRIMER_LEFT_127_TM=59.370 PRIMER_RIGHT_127_TM=59.396 PRIMER_LEFT_127_GC_PERCENT=52.632 PRIMER_RIGHT_127_GC_PERCENT=47.368 PRIMER_LEFT_127_SELF_ANY=5.00 PRIMER_RIGHT_127_SELF_ANY=4.00 PRIMER_LEFT_127_SELF_END=2.00 PRIMER_RIGHT_127_SELF_END=0.00 PRIMER_LEFT_127_END_STABILITY=8.4000 PRIMER_RIGHT_127_END_STABILITY=9.4000 PRIMER_PAIR_127_COMPL_ANY=5.00 PRIMER_PAIR_127_COMPL_END=2.00 PRIMER_PAIR_127_PRODUCT_SIZE=78 PRIMER_PAIR_128_PENALTY=3.239449 PRIMER_LEFT_128_PENALTY=1.810378 PRIMER_RIGHT_128_PENALTY=1.429072 PRIMER_LEFT_128_SEQUENCE=CATCCAGAACTGGTGCAAGC PRIMER_RIGHT_128_SEQUENCE=AAACTCACCAACTAAGCAGCG PRIMER_LEFT_128=478,20 PRIMER_RIGHT_128=565,21 PRIMER_LEFT_128_TM=61.810 PRIMER_RIGHT_128_TM=59.571 PRIMER_LEFT_128_GC_PERCENT=55.000 PRIMER_RIGHT_128_GC_PERCENT=47.619 PRIMER_LEFT_128_SELF_ANY=6.00 PRIMER_RIGHT_128_SELF_ANY=3.00 PRIMER_LEFT_128_SELF_END=2.00 PRIMER_RIGHT_128_SELF_END=2.00 PRIMER_LEFT_128_END_STABILITY=8.5000 PRIMER_RIGHT_128_END_STABILITY=10.2000 PRIMER_PAIR_128_COMPL_ANY=5.00 PRIMER_PAIR_128_COMPL_END=1.00 PRIMER_PAIR_128_PRODUCT_SIZE=88 PRIMER_PAIR_129_PENALTY=3.247580 PRIMER_LEFT_129_PENALTY=1.429072 PRIMER_RIGHT_129_PENALTY=1.818509 PRIMER_LEFT_129_SEQUENCE=CGCTGCTTAGTTGGTGAGTTT PRIMER_RIGHT_129_SEQUENCE=TCGCAAACATCCATCCTCT PRIMER_LEFT_129=545,21 PRIMER_RIGHT_129=633,19 PRIMER_LEFT_129_TM=59.571 PRIMER_RIGHT_129_TM=59.181 PRIMER_LEFT_129_GC_PERCENT=47.619 PRIMER_RIGHT_129_GC_PERCENT=47.368 PRIMER_LEFT_129_SELF_ANY=3.00 PRIMER_RIGHT_129_SELF_ANY=2.00 PRIMER_LEFT_129_SELF_END=0.00 PRIMER_RIGHT_129_SELF_END=0.00 PRIMER_LEFT_129_END_STABILITY=6.7000 PRIMER_RIGHT_129_END_STABILITY=7.9000 PRIMER_PAIR_129_COMPL_ANY=4.00 PRIMER_PAIR_129_COMPL_END=3.00 PRIMER_PAIR_129_PRODUCT_SIZE=89 PRIMER_PAIR_130_PENALTY=3.275628 PRIMER_LEFT_130_PENALTY=0.860239 PRIMER_RIGHT_130_PENALTY=2.415388 PRIMER_LEFT_130_SEQUENCE=CAGATCCATCAGGGACCAAA PRIMER_RIGHT_130_SEQUENCE=TCTACCACATTGGTGATCTGC PRIMER_LEFT_130=360,20 PRIMER_RIGHT_130=459,21 PRIMER_LEFT_130_TM=60.860 PRIMER_RIGHT_130_TM=58.585 PRIMER_LEFT_130_GC_PERCENT=50.000 PRIMER_RIGHT_130_GC_PERCENT=47.619 PRIMER_LEFT_130_SELF_ANY=5.00 PRIMER_RIGHT_130_SELF_ANY=6.00 PRIMER_LEFT_130_SELF_END=0.00 PRIMER_RIGHT_130_SELF_END=2.00 PRIMER_LEFT_130_END_STABILITY=8.8000 PRIMER_RIGHT_130_END_STABILITY=8.2000 PRIMER_PAIR_130_COMPL_ANY=6.00 PRIMER_PAIR_130_COMPL_END=2.00 PRIMER_PAIR_130_PRODUCT_SIZE=100 PRIMER_PAIR_131_PENALTY=3.277129 PRIMER_LEFT_131_PENALTY=2.613581 PRIMER_RIGHT_131_PENALTY=0.663548 PRIMER_LEFT_131_SEQUENCE=CTGGCTGAACCCCAGATTG PRIMER_RIGHT_131_SEQUENCE=TCTGAATCCCACTTCCCATT PRIMER_LEFT_131=284,19 PRIMER_RIGHT_131=363,20 PRIMER_LEFT_131_TM=61.614 PRIMER_RIGHT_131_TM=59.336 PRIMER_LEFT_131_GC_PERCENT=57.895 PRIMER_RIGHT_131_GC_PERCENT=45.000 PRIMER_LEFT_131_SELF_ANY=4.00 PRIMER_RIGHT_131_SELF_ANY=3.00 PRIMER_LEFT_131_SELF_END=2.00 PRIMER_RIGHT_131_SELF_END=1.00 PRIMER_LEFT_131_END_STABILITY=6.9000 PRIMER_RIGHT_131_END_STABILITY=8.4000 PRIMER_PAIR_131_COMPL_ANY=4.00 PRIMER_PAIR_131_COMPL_END=2.00 PRIMER_PAIR_131_PRODUCT_SIZE=80 PRIMER_PAIR_132_PENALTY=3.298886 PRIMER_LEFT_132_PENALTY=1.446550 PRIMER_RIGHT_132_PENALTY=1.852336 PRIMER_LEFT_132_SEQUENCE=TACCGCTGCTTAGTTGGTGAG PRIMER_RIGHT_132_SEQUENCE=CCATCCTCTCCTGGTGTAAGA PRIMER_LEFT_132=542,21 PRIMER_RIGHT_132=623,21 PRIMER_LEFT_132_TM=60.447 PRIMER_RIGHT_132_TM=59.148 PRIMER_LEFT_132_GC_PERCENT=52.381 PRIMER_RIGHT_132_GC_PERCENT=52.381 PRIMER_LEFT_132_SELF_ANY=3.00 PRIMER_RIGHT_132_SELF_ANY=3.00 PRIMER_LEFT_132_SELF_END=0.00 PRIMER_RIGHT_132_SELF_END=3.00 PRIMER_LEFT_132_END_STABILITY=6.4000 PRIMER_RIGHT_132_END_STABILITY=6.0000 PRIMER_PAIR_132_COMPL_ANY=4.00 PRIMER_PAIR_132_COMPL_END=0.00 PRIMER_PAIR_132_PRODUCT_SIZE=82 PRIMER_PAIR_133_PENALTY=3.298886 PRIMER_LEFT_133_PENALTY=1.446550 PRIMER_RIGHT_133_PENALTY=1.852336 PRIMER_LEFT_133_SEQUENCE=CTACCGCTGCTTAGTTGGTGA PRIMER_RIGHT_133_SEQUENCE=TCCATCCTCTCCTGGTGTAAG PRIMER_LEFT_133=541,21 PRIMER_RIGHT_133=624,21 PRIMER_LEFT_133_TM=60.447 PRIMER_RIGHT_133_TM=59.148 PRIMER_LEFT_133_GC_PERCENT=52.381 PRIMER_RIGHT_133_GC_PERCENT=52.381 PRIMER_LEFT_133_SELF_ANY=3.00 PRIMER_RIGHT_133_SELF_ANY=3.00 PRIMER_LEFT_133_SELF_END=1.00 PRIMER_RIGHT_133_SELF_END=1.00 PRIMER_LEFT_133_END_STABILITY=7.9000 PRIMER_RIGHT_133_END_STABILITY=5.7000 PRIMER_PAIR_133_COMPL_ANY=4.00 PRIMER_PAIR_133_COMPL_END=0.00 PRIMER_PAIR_133_PRODUCT_SIZE=84 PRIMER_PAIR_134_PENALTY=3.306030 PRIMER_LEFT_134_PENALTY=1.675600 PRIMER_RIGHT_134_PENALTY=1.630431 PRIMER_LEFT_134_SEQUENCE=TGGAGGTACCCACTGATGG PRIMER_RIGHT_134_SEQUENCE=TGCATGTTCAGTCTGCCAC PRIMER_LEFT_134=252,19 PRIMER_RIGHT_134=330,19 PRIMER_LEFT_134_TM=59.324 PRIMER_RIGHT_134_TM=59.370 PRIMER_LEFT_134_GC_PERCENT=57.895 PRIMER_RIGHT_134_GC_PERCENT=52.632 PRIMER_LEFT_134_SELF_ANY=6.00 PRIMER_RIGHT_134_SELF_ANY=5.00 PRIMER_LEFT_134_SELF_END=3.00 PRIMER_RIGHT_134_SELF_END=3.00 PRIMER_LEFT_134_END_STABILITY=8.1000 PRIMER_RIGHT_134_END_STABILITY=9.4000 PRIMER_PAIR_134_COMPL_ANY=5.00 PRIMER_PAIR_134_COMPL_END=2.00 PRIMER_PAIR_134_PRODUCT_SIZE=79 PRIMER_PAIR_135_PENALTY=3.310502 PRIMER_LEFT_135_PENALTY=2.051254 PRIMER_RIGHT_135_PENALTY=1.259248 PRIMER_LEFT_135_SEQUENCE=GAACCCCAGATTGCCATGTT PRIMER_RIGHT_135_SEQUENCE=GGTCCCTGATGGATCTGAAT PRIMER_LEFT_135=290,20 PRIMER_RIGHT_135=376,20 PRIMER_LEFT_135_TM=62.051 PRIMER_RIGHT_135_TM=58.741 PRIMER_LEFT_135_GC_PERCENT=50.000 PRIMER_RIGHT_135_GC_PERCENT=50.000 PRIMER_LEFT_135_SELF_ANY=4.00 PRIMER_RIGHT_135_SELF_ANY=5.00 PRIMER_LEFT_135_SELF_END=2.00 PRIMER_RIGHT_135_SELF_END=2.00 PRIMER_LEFT_135_END_STABILITY=6.6000 PRIMER_RIGHT_135_END_STABILITY=6.9000 PRIMER_PAIR_135_COMPL_ANY=5.00 PRIMER_PAIR_135_COMPL_END=1.00 PRIMER_PAIR_135_PRODUCT_SIZE=87 PRIMER_PAIR_136_PENALTY=3.324260 PRIMER_LEFT_136_PENALTY=0.663548 PRIMER_RIGHT_136_PENALTY=2.660713 PRIMER_LEFT_136_SEQUENCE=GAATGGGAAGTGGGATTCAG PRIMER_RIGHT_136_SEQUENCE=GTTCAGGGTAGACTTCTTGGCA PRIMER_LEFT_136=343,20 PRIMER_RIGHT_136=437,22 PRIMER_LEFT_136_TM=59.336 PRIMER_RIGHT_136_TM=60.661 PRIMER_LEFT_136_GC_PERCENT=50.000 PRIMER_RIGHT_136_GC_PERCENT=50.000 PRIMER_LEFT_136_SELF_ANY=4.00 PRIMER_RIGHT_136_SELF_ANY=4.00 PRIMER_LEFT_136_SELF_END=1.00 PRIMER_RIGHT_136_SELF_END=3.00 PRIMER_LEFT_136_END_STABILITY=7.0000 PRIMER_RIGHT_136_END_STABILITY=10.0000 PRIMER_PAIR_136_COMPL_ANY=5.00 PRIMER_PAIR_136_COMPL_END=0.00 PRIMER_PAIR_136_PRODUCT_SIZE=95 PRIMER_PAIR_137_PENALTY=3.324495 PRIMER_LEFT_137_PENALTY=1.811785 PRIMER_RIGHT_137_PENALTY=1.512710 PRIMER_LEFT_137_SEQUENCE=GGTACCCACTGATGGTAATGC PRIMER_RIGHT_137_SEQUENCE=CTTCCCATTCTGGACATTCA PRIMER_LEFT_137=256,21 PRIMER_RIGHT_137=352,20 PRIMER_LEFT_137_TM=59.188 PRIMER_RIGHT_137_TM=58.487 PRIMER_LEFT_137_GC_PERCENT=52.381 PRIMER_RIGHT_137_GC_PERCENT=45.000 PRIMER_LEFT_137_SELF_ANY=6.00 PRIMER_RIGHT_137_SELF_ANY=3.00 PRIMER_LEFT_137_SELF_END=2.00 PRIMER_RIGHT_137_SELF_END=2.00 PRIMER_LEFT_137_END_STABILITY=8.4000 PRIMER_RIGHT_137_END_STABILITY=6.9000 PRIMER_PAIR_137_COMPL_ANY=5.00 PRIMER_PAIR_137_COMPL_END=2.00 PRIMER_PAIR_137_PRODUCT_SIZE=97 PRIMER_PAIR_138_PENALTY=3.329952 PRIMER_LEFT_138_PENALTY=2.212679 PRIMER_RIGHT_138_PENALTY=1.117273 PRIMER_LEFT_138_SEQUENCE=CCGTCGCCAAAGAGACAT PRIMER_RIGHT_138_SEQUENCE=CTCTACCCCTCGGAACTTGTC PRIMER_LEFT_138=654,18 PRIMER_RIGHT_138=748,21 PRIMER_LEFT_138_TM=59.787 PRIMER_RIGHT_138_TM=60.117 PRIMER_LEFT_138_GC_PERCENT=55.556 PRIMER_RIGHT_138_GC_PERCENT=57.143 PRIMER_LEFT_138_SELF_ANY=3.00 PRIMER_RIGHT_138_SELF_ANY=3.00 PRIMER_LEFT_138_SELF_END=2.00 PRIMER_RIGHT_138_SELF_END=1.00 PRIMER_LEFT_138_END_STABILITY=6.3000 PRIMER_RIGHT_138_END_STABILITY=6.7000 PRIMER_PAIR_138_COMPL_ANY=4.00 PRIMER_PAIR_138_COMPL_END=3.00 PRIMER_PAIR_138_PRODUCT_SIZE=95 PRIMER_PAIR_139_PENALTY=3.332276 PRIMER_LEFT_139_PENALTY=0.663548 PRIMER_RIGHT_139_PENALTY=2.668729 PRIMER_LEFT_139_SEQUENCE=AGAATGGGAAGTGGGATTCA PRIMER_RIGHT_139_SEQUENCE=CAGGGTAGACTTCTTGGCAAT PRIMER_LEFT_139=342,20 PRIMER_RIGHT_139=434,21 PRIMER_LEFT_139_TM=59.336 PRIMER_RIGHT_139_TM=58.331 PRIMER_LEFT_139_GC_PERCENT=45.000 PRIMER_RIGHT_139_GC_PERCENT=47.619 PRIMER_LEFT_139_SELF_ANY=4.00 PRIMER_RIGHT_139_SELF_ANY=5.00 PRIMER_LEFT_139_SELF_END=1.00 PRIMER_RIGHT_139_SELF_END=3.00 PRIMER_LEFT_139_END_STABILITY=6.9000 PRIMER_RIGHT_139_END_STABILITY=8.4000 PRIMER_PAIR_139_COMPL_ANY=5.00 PRIMER_PAIR_139_COMPL_END=1.00 PRIMER_PAIR_139_PRODUCT_SIZE=93 PRIMER_PAIR_140_PENALTY=3.349242 PRIMER_LEFT_140_PENALTY=1.782887 PRIMER_RIGHT_140_PENALTY=1.566355 PRIMER_LEFT_140_SEQUENCE=AGACCCATCCCCACTTTGT PRIMER_RIGHT_140_SEQUENCE=GTCAGGAACGAGAAGGGCAT PRIMER_LEFT_140=516,19 PRIMER_RIGHT_140=592,20 PRIMER_LEFT_140_TM=59.217 PRIMER_RIGHT_140_TM=61.566 PRIMER_LEFT_140_GC_PERCENT=52.632 PRIMER_RIGHT_140_GC_PERCENT=55.000 PRIMER_LEFT_140_SELF_ANY=2.00 PRIMER_RIGHT_140_SELF_ANY=2.00 PRIMER_LEFT_140_SELF_END=1.00 PRIMER_RIGHT_140_SELF_END=2.00 PRIMER_LEFT_140_END_STABILITY=7.0000 PRIMER_RIGHT_140_END_STABILITY=9.6000 PRIMER_PAIR_140_COMPL_ANY=4.00 PRIMER_PAIR_140_COMPL_END=3.00 PRIMER_PAIR_140_PRODUCT_SIZE=77 PRIMER_PAIR_141_PENALTY=3.352040 PRIMER_LEFT_141_PENALTY=1.065099 PRIMER_RIGHT_141_PENALTY=2.286941 PRIMER_LEFT_141_SEQUENCE=TACACCAGGAGAGGATGGATG PRIMER_RIGHT_141_SEQUENCE=CCGTAGTCATGCAAGTTGGTA PRIMER_LEFT_141=606,21 PRIMER_RIGHT_141=705,21 PRIMER_LEFT_141_TM=59.935 PRIMER_RIGHT_141_TM=58.713 PRIMER_LEFT_141_GC_PERCENT=52.381 PRIMER_RIGHT_141_GC_PERCENT=47.619 PRIMER_LEFT_141_SELF_ANY=3.00 PRIMER_RIGHT_141_SELF_ANY=5.00 PRIMER_LEFT_141_SELF_END=0.00 PRIMER_RIGHT_141_SELF_END=2.00 PRIMER_LEFT_141_END_STABILITY=8.1000 PRIMER_RIGHT_141_END_STABILITY=7.2000 PRIMER_PAIR_141_COMPL_ANY=4.00 PRIMER_PAIR_141_COMPL_END=0.00 PRIMER_PAIR_141_PRODUCT_SIZE=100 PRIMER_PAIR_142_PENALTY=3.399919 PRIMER_LEFT_142_PENALTY=1.544828 PRIMER_RIGHT_142_PENALTY=1.855091 PRIMER_LEFT_142_SEQUENCE=ACCGCTGCTTAGTTGGTGA PRIMER_RIGHT_142_SEQUENCE=GAGTTTCGCAAACATCCATC PRIMER_LEFT_142=543,19 PRIMER_RIGHT_142=638,20 PRIMER_LEFT_142_TM=59.455 PRIMER_RIGHT_142_TM=58.145 PRIMER_LEFT_142_GC_PERCENT=52.632 PRIMER_RIGHT_142_GC_PERCENT=45.000 PRIMER_LEFT_142_SELF_ANY=3.00 PRIMER_RIGHT_142_SELF_ANY=6.00 PRIMER_LEFT_142_SELF_END=1.00 PRIMER_RIGHT_142_SELF_END=0.00 PRIMER_LEFT_142_END_STABILITY=7.9000 PRIMER_RIGHT_142_END_STABILITY=8.1000 PRIMER_PAIR_142_COMPL_ANY=4.00 PRIMER_PAIR_142_COMPL_END=3.00 PRIMER_PAIR_142_PRODUCT_SIZE=96 PRIMER_PAIR_143_PENALTY=3.414813 PRIMER_LEFT_143_PENALTY=1.968263 PRIMER_RIGHT_143_PENALTY=1.446550 PRIMER_LEFT_143_SEQUENCE=CCAACCAGTGACCATCCAGA PRIMER_RIGHT_143_SEQUENCE=TCACCAACTAAGCAGCGGTAG PRIMER_LEFT_143=466,20 PRIMER_RIGHT_143=561,21 PRIMER_LEFT_143_TM=61.968 PRIMER_RIGHT_143_TM=60.447 PRIMER_LEFT_143_GC_PERCENT=55.000 PRIMER_RIGHT_143_GC_PERCENT=52.381 PRIMER_LEFT_143_SELF_ANY=3.00 PRIMER_RIGHT_143_SELF_ANY=3.00 PRIMER_LEFT_143_SELF_END=2.00 PRIMER_RIGHT_143_SELF_END=3.00 PRIMER_LEFT_143_END_STABILITY=8.2000 PRIMER_RIGHT_143_END_STABILITY=6.9000 PRIMER_PAIR_143_COMPL_ANY=4.00 PRIMER_PAIR_143_COMPL_END=0.00 PRIMER_PAIR_143_PRODUCT_SIZE=96 PRIMER_PAIR_144_PENALTY=3.433919 PRIMER_LEFT_144_PENALTY=2.182723 PRIMER_RIGHT_144_PENALTY=1.251196 PRIMER_LEFT_144_SEQUENCE=AAGCAGTGCAAGACCCATC PRIMER_RIGHT_144_SEQUENCE=ACGAGAAGGGCATCACTTACA PRIMER_LEFT_144=506,19 PRIMER_RIGHT_144=585,21 PRIMER_LEFT_144_TM=58.817 PRIMER_RIGHT_144_TM=59.749 PRIMER_LEFT_144_GC_PERCENT=52.632 PRIMER_RIGHT_144_GC_PERCENT=47.619 PRIMER_LEFT_144_SELF_ANY=5.00 PRIMER_RIGHT_144_SELF_ANY=4.00 PRIMER_LEFT_144_SELF_END=0.00 PRIMER_RIGHT_144_SELF_END=0.00 PRIMER_LEFT_144_END_STABILITY=8.1000 PRIMER_RIGHT_144_END_STABILITY=6.0000 PRIMER_PAIR_144_COMPL_ANY=5.00 PRIMER_PAIR_144_COMPL_END=2.00 PRIMER_PAIR_144_PRODUCT_SIZE=80 PRIMER_PAIR_145_PENALTY=3.452176 PRIMER_LEFT_145_PENALTY=2.389824 PRIMER_RIGHT_145_PENALTY=1.062353 PRIMER_LEFT_145_SEQUENCE=TTGATACCAAGGAAGGCATC PRIMER_RIGHT_145_SEQUENCE=CTGGATGGTCACTGGTTGG PRIMER_LEFT_145=387,20 PRIMER_RIGHT_145=484,19 PRIMER_LEFT_145_TM=57.610 PRIMER_RIGHT_145_TM=59.938 PRIMER_LEFT_145_GC_PERCENT=45.000 PRIMER_RIGHT_145_GC_PERCENT=57.895 PRIMER_LEFT_145_SELF_ANY=6.00 PRIMER_RIGHT_145_SELF_ANY=3.00 PRIMER_LEFT_145_SELF_END=3.00 PRIMER_RIGHT_145_SELF_END=0.00 PRIMER_LEFT_145_END_STABILITY=8.1000 PRIMER_RIGHT_145_END_STABILITY=8.2000 PRIMER_PAIR_145_COMPL_ANY=4.00 PRIMER_PAIR_145_COMPL_END=2.00 PRIMER_PAIR_145_PRODUCT_SIZE=98 PRIMER_PAIR_146_PENALTY=3.461868 PRIMER_LEFT_146_PENALTY=1.533205 PRIMER_RIGHT_146_PENALTY=1.928663 PRIMER_LEFT_146_SEQUENCE=GTGATTCCCTACCGCTGCTT PRIMER_RIGHT_146_SEQUENCE=TCCATCCTCTCCTGGTGTAA PRIMER_LEFT_146=533,20 PRIMER_RIGHT_146=624,20 PRIMER_LEFT_146_TM=61.533 PRIMER_RIGHT_146_TM=58.071 PRIMER_LEFT_146_GC_PERCENT=55.000 PRIMER_RIGHT_146_GC_PERCENT=50.000 PRIMER_LEFT_146_SELF_ANY=3.00 PRIMER_RIGHT_146_SELF_ANY=3.00 PRIMER_LEFT_146_SELF_END=0.00 PRIMER_RIGHT_146_SELF_END=1.00 PRIMER_LEFT_146_END_STABILITY=8.5000 PRIMER_RIGHT_146_END_STABILITY=6.0000 PRIMER_PAIR_146_COMPL_ANY=3.00 PRIMER_PAIR_146_COMPL_END=2.00 PRIMER_PAIR_146_PRODUCT_SIZE=92 PRIMER_PAIR_147_PENALTY=3.465550 PRIMER_LEFT_147_PENALTY=2.056428 PRIMER_RIGHT_147_PENALTY=1.409123 PRIMER_LEFT_147_SEQUENCE=CCAAAACCTGCATTGATACC PRIMER_RIGHT_147_SEQUENCE=ACTGGTTGGTTGGCTTCTACC PRIMER_LEFT_147=375,20 PRIMER_RIGHT_147=474,21 PRIMER_LEFT_147_TM=57.944 PRIMER_RIGHT_147_TM=60.409 PRIMER_LEFT_147_GC_PERCENT=45.000 PRIMER_RIGHT_147_GC_PERCENT=52.381 PRIMER_LEFT_147_SELF_ANY=4.00 PRIMER_RIGHT_147_SELF_ANY=3.00 PRIMER_LEFT_147_SELF_END=2.00 PRIMER_RIGHT_147_SELF_END=2.00 PRIMER_LEFT_147_END_STABILITY=6.8000 PRIMER_RIGHT_147_END_STABILITY=6.9000 PRIMER_PAIR_147_COMPL_ANY=4.00 PRIMER_PAIR_147_COMPL_END=0.00 PRIMER_PAIR_147_PRODUCT_SIZE=100 PRIMER_PAIR_148_PENALTY=3.469999 PRIMER_LEFT_148_PENALTY=2.214324 PRIMER_RIGHT_148_PENALTY=1.255675 PRIMER_LEFT_148_SEQUENCE=GCAGTGCAAGACCCATCC PRIMER_RIGHT_148_SEQUENCE=CGAGAAGGGCATCACTTACAA PRIMER_LEFT_148=508,18 PRIMER_RIGHT_148=584,21 PRIMER_LEFT_148_TM=60.214 PRIMER_RIGHT_148_TM=60.256 PRIMER_LEFT_148_GC_PERCENT=61.111 PRIMER_RIGHT_148_GC_PERCENT=47.619 PRIMER_LEFT_148_SELF_ANY=5.00 PRIMER_RIGHT_148_SELF_ANY=4.00 PRIMER_LEFT_148_SELF_END=0.00 PRIMER_RIGHT_148_SELF_END=2.00 PRIMER_LEFT_148_END_STABILITY=8.1000 PRIMER_RIGHT_148_END_STABILITY=6.0000 PRIMER_PAIR_148_COMPL_ANY=5.00 PRIMER_PAIR_148_COMPL_END=2.00 PRIMER_PAIR_148_PRODUCT_SIZE=77 PRIMER_PAIR_149_PENALTY=3.507898 PRIMER_LEFT_149_PENALTY=1.923751 PRIMER_RIGHT_149_PENALTY=1.584147 PRIMER_LEFT_149_SEQUENCE=ATGTGGTAGAAGCCAACCAA PRIMER_RIGHT_149_SEQUENCE=ATCACAAAGTGGGGATGGG PRIMER_LEFT_149=450,20 PRIMER_RIGHT_149=537,19 PRIMER_LEFT_149_TM=58.076 PRIMER_RIGHT_149_TM=60.584 PRIMER_LEFT_149_GC_PERCENT=45.000 PRIMER_RIGHT_149_GC_PERCENT=52.632 PRIMER_LEFT_149_SELF_ANY=4.00 PRIMER_RIGHT_149_SELF_ANY=4.00 PRIMER_LEFT_149_SELF_END=2.00 PRIMER_RIGHT_149_SELF_END=0.00 PRIMER_LEFT_149_END_STABILITY=8.2000 PRIMER_RIGHT_149_END_STABILITY=9.6000 PRIMER_PAIR_149_COMPL_ANY=4.00 PRIMER_PAIR_149_COMPL_END=2.00 PRIMER_PAIR_149_PRODUCT_SIZE=88 PRIMER_PAIR_150_PENALTY=3.511514 PRIMER_LEFT_150_PENALTY=2.071163 PRIMER_RIGHT_150_PENALTY=1.440351 PRIMER_LEFT_150_SEQUENCE=ATTGCCATGTTCTGTGGCAG PRIMER_RIGHT_150_SEQUENCE=TGGTCCCTGATGGATCTGAA PRIMER_LEFT_150=299,20 PRIMER_RIGHT_150=377,20 PRIMER_LEFT_150_TM=62.071 PRIMER_RIGHT_150_TM=61.440 PRIMER_LEFT_150_GC_PERCENT=50.000 PRIMER_RIGHT_150_GC_PERCENT=50.000 PRIMER_LEFT_150_SELF_ANY=5.00 PRIMER_RIGHT_150_SELF_ANY=5.00 PRIMER_LEFT_150_SELF_END=3.00 PRIMER_RIGHT_150_SELF_END=1.00 PRIMER_LEFT_150_END_STABILITY=9.7000 PRIMER_RIGHT_150_END_STABILITY=7.0000 PRIMER_PAIR_150_COMPL_ANY=4.00 PRIMER_PAIR_150_COMPL_END=1.00 PRIMER_PAIR_150_PRODUCT_SIZE=79 PRIMER_PAIR_151_PENALTY=3.515712 PRIMER_LEFT_151_PENALTY=1.982507 PRIMER_RIGHT_151_PENALTY=1.533205 PRIMER_LEFT_151_SEQUENCE=AGCCAACCAACCAGTGACC PRIMER_RIGHT_151_SEQUENCE=AAGCAGCGGTAGGGAATCAC PRIMER_LEFT_151=460,19 PRIMER_RIGHT_151=552,20 PRIMER_LEFT_151_TM=60.983 PRIMER_RIGHT_151_TM=61.533 PRIMER_LEFT_151_GC_PERCENT=57.895 PRIMER_RIGHT_151_GC_PERCENT=55.000 PRIMER_LEFT_151_SELF_ANY=3.00 PRIMER_RIGHT_151_SELF_ANY=3.00 PRIMER_LEFT_151_SELF_END=1.00 PRIMER_RIGHT_151_SELF_END=3.00 PRIMER_LEFT_151_END_STABILITY=7.9000 PRIMER_RIGHT_151_END_STABILITY=6.3000 PRIMER_PAIR_151_COMPL_ANY=4.00 PRIMER_PAIR_151_COMPL_END=2.00 PRIMER_PAIR_151_PRODUCT_SIZE=93 PRIMER_PAIR_152_PENALTY=3.519008 PRIMER_LEFT_152_PENALTY=1.923751 PRIMER_RIGHT_152_PENALTY=1.595257 PRIMER_LEFT_152_SEQUENCE=CAATGTGGTAGAAGCCAACC PRIMER_RIGHT_152_SEQUENCE=GTAGGGAATCACAAAGTGGGG PRIMER_LEFT_152=448,20 PRIMER_RIGHT_152=544,21 PRIMER_LEFT_152_TM=58.076 PRIMER_RIGHT_152_TM=60.595 PRIMER_LEFT_152_GC_PERCENT=50.000 PRIMER_RIGHT_152_GC_PERCENT=52.381 PRIMER_LEFT_152_SELF_ANY=3.00 PRIMER_RIGHT_152_SELF_ANY=3.00 PRIMER_LEFT_152_SELF_END=2.00 PRIMER_RIGHT_152_SELF_END=0.00 PRIMER_LEFT_152_END_STABILITY=8.2000 PRIMER_RIGHT_152_END_STABILITY=11.2000 PRIMER_PAIR_152_COMPL_ANY=4.00 PRIMER_PAIR_152_COMPL_END=2.00 PRIMER_PAIR_152_PRODUCT_SIZE=97 PRIMER_PAIR_153_PENALTY=3.519008 PRIMER_LEFT_153_PENALTY=1.923751 PRIMER_RIGHT_153_PENALTY=1.595257 PRIMER_LEFT_153_SEQUENCE=CCAATGTGGTAGAAGCCAAC PRIMER_RIGHT_153_SEQUENCE=GGTAGGGAATCACAAAGTGGG PRIMER_LEFT_153=447,20 PRIMER_RIGHT_153=545,21 PRIMER_LEFT_153_TM=58.076 PRIMER_RIGHT_153_TM=60.595 PRIMER_LEFT_153_GC_PERCENT=50.000 PRIMER_RIGHT_153_GC_PERCENT=52.381 PRIMER_LEFT_153_SELF_ANY=4.00 PRIMER_RIGHT_153_SELF_ANY=3.00 PRIMER_LEFT_153_SELF_END=0.00 PRIMER_RIGHT_153_SELF_END=1.00 PRIMER_LEFT_153_END_STABILITY=8.2000 PRIMER_RIGHT_153_END_STABILITY=9.4000 PRIMER_PAIR_153_COMPL_ANY=4.00 PRIMER_PAIR_153_COMPL_END=3.00 PRIMER_PAIR_153_PRODUCT_SIZE=99 PRIMER_PAIR_154_PENALTY=3.528238 PRIMER_LEFT_154_PENALTY=1.604487 PRIMER_RIGHT_154_PENALTY=1.923751 PRIMER_LEFT_154_SEQUENCE=AGGGACCAAAACCTGCATT PRIMER_RIGHT_154_SEQUENCE=GGTTGGCTTCTACCACATTG PRIMER_LEFT_154=370,19 PRIMER_RIGHT_154=467,20 PRIMER_LEFT_154_TM=59.396 PRIMER_RIGHT_154_TM=58.076 PRIMER_LEFT_154_GC_PERCENT=47.368 PRIMER_RIGHT_154_GC_PERCENT=50.000 PRIMER_LEFT_154_SELF_ANY=4.00 PRIMER_RIGHT_154_SELF_ANY=3.00 PRIMER_LEFT_154_SELF_END=1.00 PRIMER_RIGHT_154_SELF_END=3.00 PRIMER_LEFT_154_END_STABILITY=8.4000 PRIMER_RIGHT_154_END_STABILITY=7.2000 PRIMER_PAIR_154_COMPL_ANY=4.00 PRIMER_PAIR_154_COMPL_END=2.00 PRIMER_PAIR_154_PRODUCT_SIZE=98 PRIMER_PAIR_155_PENALTY=3.560259 PRIMER_LEFT_155_PENALTY=3.250857 PRIMER_RIGHT_155_PENALTY=0.309402 PRIMER_LEFT_155_SEQUENCE=GCGCTGGAGGTACCCACT PRIMER_RIGHT_155_SEQUENCE=CAGTCTGCCACAGAACATGG PRIMER_LEFT_155=248,18 PRIMER_RIGHT_155=322,20 PRIMER_LEFT_155_TM=61.251 PRIMER_RIGHT_155_TM=60.309 PRIMER_LEFT_155_GC_PERCENT=66.667 PRIMER_RIGHT_155_GC_PERCENT=55.000 PRIMER_LEFT_155_SELF_ANY=6.00 PRIMER_RIGHT_155_SELF_ANY=5.00 PRIMER_LEFT_155_SELF_END=2.00 PRIMER_RIGHT_155_SELF_END=2.00 PRIMER_LEFT_155_END_STABILITY=7.9000 PRIMER_RIGHT_155_END_STABILITY=8.4000 PRIMER_PAIR_155_COMPL_ANY=3.00 PRIMER_PAIR_155_COMPL_END=2.00 PRIMER_PAIR_155_PRODUCT_SIZE=75 PRIMER_PAIR_156_PENALTY=3.568662 PRIMER_LEFT_156_PENALTY=1.902968 PRIMER_RIGHT_156_PENALTY=1.665693 PRIMER_LEFT_156_SEQUENCE=CCCATCCCCACTTTGTGATT PRIMER_RIGHT_156_SEQUENCE=ACTTGTCAGGAACGAGAAGGG PRIMER_LEFT_156=519,20 PRIMER_RIGHT_156=596,21 PRIMER_LEFT_156_TM=61.903 PRIMER_RIGHT_156_TM=60.666 PRIMER_LEFT_156_GC_PERCENT=50.000 PRIMER_RIGHT_156_GC_PERCENT=52.381 PRIMER_LEFT_156_SELF_ANY=4.00 PRIMER_RIGHT_156_SELF_ANY=4.00 PRIMER_LEFT_156_SELF_END=2.00 PRIMER_RIGHT_156_SELF_END=0.00 PRIMER_LEFT_156_END_STABILITY=6.9000 PRIMER_RIGHT_156_END_STABILITY=9.7000 PRIMER_PAIR_156_COMPL_ANY=4.00 PRIMER_PAIR_156_COMPL_END=1.00 PRIMER_PAIR_156_PRODUCT_SIZE=78 PRIMER_PAIR_157_PENALTY=3.575421 PRIMER_LEFT_157_PENALTY=1.923751 PRIMER_RIGHT_157_PENALTY=1.651669 PRIMER_LEFT_157_SEQUENCE=ACCAATGTGGTAGAAGCCAA PRIMER_RIGHT_157_SEQUENCE=AGGGAATCACAAAGTGGGGA PRIMER_LEFT_157=446,20 PRIMER_RIGHT_157=542,20 PRIMER_LEFT_157_TM=58.076 PRIMER_RIGHT_157_TM=61.652 PRIMER_LEFT_157_GC_PERCENT=45.000 PRIMER_RIGHT_157_GC_PERCENT=50.000 PRIMER_LEFT_157_SELF_ANY=6.00 PRIMER_RIGHT_157_SELF_ANY=3.00 PRIMER_LEFT_157_SELF_END=0.00 PRIMER_RIGHT_157_SELF_END=2.00 PRIMER_LEFT_157_END_STABILITY=10.0000 PRIMER_RIGHT_157_END_STABILITY=10.9000 PRIMER_PAIR_157_COMPL_ANY=4.00 PRIMER_PAIR_157_COMPL_END=0.00 PRIMER_PAIR_157_PRODUCT_SIZE=97 PRIMER_PAIR_158_PENALTY=3.616048 PRIMER_LEFT_158_PENALTY=1.665693 PRIMER_RIGHT_158_PENALTY=1.950354 PRIMER_LEFT_158_SEQUENCE=CCCTTCTCGTTCCTGACAAGT PRIMER_RIGHT_158_SEQUENCE=CTGCATGTCTCTTTGGCGAC PRIMER_LEFT_158=576,21 PRIMER_RIGHT_158=675,20 PRIMER_LEFT_158_TM=60.666 PRIMER_RIGHT_158_TM=61.950 PRIMER_LEFT_158_GC_PERCENT=52.381 PRIMER_RIGHT_158_GC_PERCENT=55.000 PRIMER_LEFT_158_SELF_ANY=4.00 PRIMER_RIGHT_158_SELF_ANY=4.00 PRIMER_LEFT_158_SELF_END=2.00 PRIMER_RIGHT_158_SELF_END=2.00 PRIMER_LEFT_158_END_STABILITY=6.7000 PRIMER_RIGHT_158_END_STABILITY=9.6000 PRIMER_PAIR_158_COMPL_ANY=4.00 PRIMER_PAIR_158_COMPL_END=2.00 PRIMER_PAIR_158_PRODUCT_SIZE=100 PRIMER_PAIR_159_PENALTY=3.629273 PRIMER_LEFT_159_PENALTY=2.182723 PRIMER_RIGHT_159_PENALTY=1.446550 PRIMER_LEFT_159_SEQUENCE=ATCCAGAACTGGTGCAAGC PRIMER_RIGHT_159_SEQUENCE=CTCACCAACTAAGCAGCGGTA PRIMER_LEFT_159=479,19 PRIMER_RIGHT_159=562,21 PRIMER_LEFT_159_TM=58.817 PRIMER_RIGHT_159_TM=60.447 PRIMER_LEFT_159_GC_PERCENT=52.632 PRIMER_RIGHT_159_GC_PERCENT=52.381 PRIMER_LEFT_159_SELF_ANY=6.00 PRIMER_RIGHT_159_SELF_ANY=3.00 PRIMER_LEFT_159_SELF_END=2.00 PRIMER_RIGHT_159_SELF_END=2.00 PRIMER_LEFT_159_END_STABILITY=8.5000 PRIMER_RIGHT_159_END_STABILITY=8.9000 PRIMER_PAIR_159_COMPL_ANY=5.00 PRIMER_PAIR_159_COMPL_END=1.00 PRIMER_PAIR_159_PRODUCT_SIZE=84 PRIMER_PAIR_160_PENALTY=3.629920 PRIMER_LEFT_160_PENALTY=1.701257 PRIMER_RIGHT_160_PENALTY=1.928663 PRIMER_LEFT_160_SEQUENCE=ATTCCCTACCGCTGCTTAGTT PRIMER_RIGHT_160_SEQUENCE=CAAACATCCATCCTCTCCTG PRIMER_LEFT_160=536,21 PRIMER_RIGHT_160=630,20 PRIMER_LEFT_160_TM=59.299 PRIMER_RIGHT_160_TM=58.071 PRIMER_LEFT_160_GC_PERCENT=47.619 PRIMER_RIGHT_160_GC_PERCENT=50.000 PRIMER_LEFT_160_SELF_ANY=3.00 PRIMER_RIGHT_160_SELF_ANY=2.00 PRIMER_LEFT_160_SELF_END=0.00 PRIMER_RIGHT_160_SELF_END=1.00 PRIMER_LEFT_160_END_STABILITY=5.7000 PRIMER_RIGHT_160_END_STABILITY=8.2000 PRIMER_PAIR_160_COMPL_ANY=3.00 PRIMER_PAIR_160_COMPL_END=0.00 PRIMER_PAIR_160_PRODUCT_SIZE=95 PRIMER_PAIR_161_PENALTY=3.633949 PRIMER_LEFT_161_PENALTY=1.683594 PRIMER_RIGHT_161_PENALTY=1.950354 PRIMER_LEFT_161_SEQUENCE=TCGTTCCTGACAAGTGCAAAT PRIMER_RIGHT_161_SEQUENCE=ACTGCATGTCTCTTTGGCGA PRIMER_LEFT_161=582,21 PRIMER_RIGHT_161=676,20 PRIMER_LEFT_161_TM=60.684 PRIMER_RIGHT_161_TM=61.950 PRIMER_LEFT_161_GC_PERCENT=42.857 PRIMER_RIGHT_161_GC_PERCENT=50.000 PRIMER_LEFT_161_SELF_ANY=4.00 PRIMER_RIGHT_161_SELF_ANY=4.00 PRIMER_LEFT_161_SELF_END=2.00 PRIMER_RIGHT_161_SELF_END=2.00 PRIMER_LEFT_161_END_STABILITY=7.2000 PRIMER_RIGHT_161_END_STABILITY=11.4000 PRIMER_PAIR_161_COMPL_ANY=4.00 PRIMER_PAIR_161_COMPL_END=1.00 PRIMER_PAIR_161_PRODUCT_SIZE=95 PRIMER_PAIR_162_PENALTY=3.637509 PRIMER_LEFT_162_PENALTY=1.928458 PRIMER_RIGHT_162_PENALTY=1.709051 PRIMER_LEFT_162_SEQUENCE=CACCAATGTGGTAGAAGCCAA PRIMER_RIGHT_162_SEQUENCE=GGGAATCACAAAGTGGGGA PRIMER_LEFT_162=445,21 PRIMER_RIGHT_162=541,19 PRIMER_LEFT_162_TM=60.928 PRIMER_RIGHT_162_TM=60.709 PRIMER_LEFT_162_GC_PERCENT=47.619 PRIMER_RIGHT_162_GC_PERCENT=52.632 PRIMER_LEFT_162_SELF_ANY=6.00 PRIMER_RIGHT_162_SELF_ANY=3.00 PRIMER_LEFT_162_SELF_END=0.00 PRIMER_RIGHT_162_SELF_END=2.00 PRIMER_LEFT_162_END_STABILITY=10.0000 PRIMER_RIGHT_162_END_STABILITY=10.9000 PRIMER_PAIR_162_COMPL_ANY=4.00 PRIMER_PAIR_162_COMPL_END=0.00 PRIMER_PAIR_162_PRODUCT_SIZE=97 PRIMER_PAIR_163_PENALTY=3.651147 PRIMER_LEFT_163_PENALTY=2.275406 PRIMER_RIGHT_163_PENALTY=1.375741 PRIMER_LEFT_163_SEQUENCE=ACACCGTCGCCAAAGAGAC PRIMER_RIGHT_163_SEQUENCE=AACTCTACCCCTCGGAACTTG PRIMER_LEFT_163=651,19 PRIMER_RIGHT_163=750,21 PRIMER_LEFT_163_TM=61.275 PRIMER_RIGHT_163_TM=59.624 PRIMER_LEFT_163_GC_PERCENT=57.895 PRIMER_RIGHT_163_GC_PERCENT=52.381 PRIMER_LEFT_163_SELF_ANY=3.00 PRIMER_RIGHT_163_SELF_ANY=3.00 PRIMER_LEFT_163_SELF_END=2.00 PRIMER_RIGHT_163_SELF_END=1.00 PRIMER_LEFT_163_END_STABILITY=6.1000 PRIMER_RIGHT_163_END_STABILITY=6.7000 PRIMER_PAIR_163_COMPL_ANY=4.00 PRIMER_PAIR_163_COMPL_END=1.00 PRIMER_PAIR_163_PRODUCT_SIZE=100 PRIMER_PAIR_164_PENALTY=3.652405 PRIMER_LEFT_164_PENALTY=1.557214 PRIMER_RIGHT_164_PENALTY=2.095192 PRIMER_LEFT_164_SEQUENCE=CGAAACTCATCTTCACTGGC PRIMER_RIGHT_164_SEQUENCE=CATGCCGTAGTCATGCAAGTT PRIMER_LEFT_164=631,20 PRIMER_RIGHT_164=709,21 PRIMER_LEFT_164_TM=58.443 PRIMER_RIGHT_164_TM=61.095 PRIMER_LEFT_164_GC_PERCENT=50.000 PRIMER_RIGHT_164_GC_PERCENT=47.619 PRIMER_LEFT_164_SELF_ANY=3.00 PRIMER_RIGHT_164_SELF_ANY=6.00 PRIMER_LEFT_164_SELF_END=2.00 PRIMER_RIGHT_164_SELF_END=3.00 PRIMER_LEFT_164_END_STABILITY=9.7000 PRIMER_RIGHT_164_END_STABILITY=6.7000 PRIMER_PAIR_164_COMPL_ANY=4.00 PRIMER_PAIR_164_COMPL_END=3.00 PRIMER_PAIR_164_PRODUCT_SIZE=79 PRIMER_PAIR_165_PENALTY=3.656409 PRIMER_LEFT_165_PENALTY=1.846031 PRIMER_RIGHT_165_PENALTY=1.810378 PRIMER_LEFT_165_SEQUENCE=GAACTGCAGATCACCAATGTG PRIMER_RIGHT_165_SEQUENCE=GATGGGTCTTGCACTGCTTG PRIMER_LEFT_165=434,21 PRIMER_RIGHT_165=524,20 PRIMER_LEFT_165_TM=59.154 PRIMER_RIGHT_165_TM=61.810 PRIMER_LEFT_165_GC_PERCENT=47.619 PRIMER_RIGHT_165_GC_PERCENT=55.000 PRIMER_LEFT_165_SELF_ANY=8.00 PRIMER_RIGHT_165_SELF_ANY=5.00 PRIMER_LEFT_165_SELF_END=3.00 PRIMER_RIGHT_165_SELF_END=3.00 PRIMER_LEFT_165_END_STABILITY=6.6000 PRIMER_RIGHT_165_END_STABILITY=8.5000 PRIMER_PAIR_165_COMPL_ANY=4.00 PRIMER_PAIR_165_COMPL_END=1.00 PRIMER_PAIR_165_PRODUCT_SIZE=91 PRIMER_PAIR_166_PENALTY=3.661578 PRIMER_LEFT_166_PENALTY=1.595257 PRIMER_RIGHT_166_PENALTY=2.066321 PRIMER_LEFT_166_SEQUENCE=CCCACTTTGTGATTCCCTACC PRIMER_RIGHT_166_SEQUENCE=TTGCACTTGTCAGGAACGA PRIMER_LEFT_166=525,21 PRIMER_RIGHT_166=600,19 PRIMER_LEFT_166_TM=60.595 PRIMER_RIGHT_166_TM=58.934 PRIMER_LEFT_166_GC_PERCENT=52.381 PRIMER_RIGHT_166_GC_PERCENT=47.368 PRIMER_LEFT_166_SELF_ANY=3.00 PRIMER_RIGHT_166_SELF_ANY=4.00 PRIMER_LEFT_166_SELF_END=0.00 PRIMER_RIGHT_166_SELF_END=1.00 PRIMER_LEFT_166_END_STABILITY=6.9000 PRIMER_RIGHT_166_END_STABILITY=8.4000 PRIMER_PAIR_166_COMPL_ANY=4.00 PRIMER_PAIR_166_COMPL_END=2.00 PRIMER_PAIR_166_PRODUCT_SIZE=76 PRIMER_PAIR_167_PENALTY=3.680580 PRIMER_LEFT_167_PENALTY=2.137489 PRIMER_RIGHT_167_PENALTY=1.543091 PRIMER_LEFT_167_SEQUENCE=GGTACCCACTGATGGTAATGCT PRIMER_RIGHT_167_SEQUENCE=CCACTTCCCATTCTGGACA PRIMER_LEFT_167=256,22 PRIMER_RIGHT_167=355,19 PRIMER_LEFT_167_TM=60.137 PRIMER_RIGHT_167_TM=59.457 PRIMER_LEFT_167_GC_PERCENT=50.000 PRIMER_RIGHT_167_GC_PERCENT=52.632 PRIMER_LEFT_167_SELF_ANY=6.00 PRIMER_RIGHT_167_SELF_ANY=3.00 PRIMER_LEFT_167_SELF_END=0.00 PRIMER_RIGHT_167_SELF_END=2.00 PRIMER_LEFT_167_END_STABILITY=8.1000 PRIMER_RIGHT_167_END_STABILITY=7.9000 PRIMER_PAIR_167_COMPL_ANY=5.00 PRIMER_PAIR_167_COMPL_END=2.00 PRIMER_PAIR_167_PRODUCT_SIZE=100 PRIMER_PAIR_168_PENALTY=3.683230 PRIMER_LEFT_168_PENALTY=1.812888 PRIMER_RIGHT_168_PENALTY=1.870342 PRIMER_LEFT_168_SEQUENCE=CGCTGCTTAGTTGGTGAGTT PRIMER_RIGHT_168_SEQUENCE=AGTTTCGCAAACATCCATCCT PRIMER_LEFT_168=545,20 PRIMER_RIGHT_168=637,21 PRIMER_LEFT_168_TM=58.187 PRIMER_RIGHT_168_TM=60.870 PRIMER_LEFT_168_GC_PERCENT=50.000 PRIMER_RIGHT_168_GC_PERCENT=42.857 PRIMER_LEFT_168_SELF_ANY=3.00 PRIMER_RIGHT_168_SELF_ANY=6.00 PRIMER_LEFT_168_SELF_END=0.00 PRIMER_RIGHT_168_SELF_END=0.00 PRIMER_LEFT_168_END_STABILITY=6.4000 PRIMER_RIGHT_168_END_STABILITY=7.8000 PRIMER_PAIR_168_COMPL_ANY=4.00 PRIMER_PAIR_168_COMPL_END=1.00 PRIMER_PAIR_168_PRODUCT_SIZE=93 PRIMER_PAIR_169_PENALTY=3.734075 PRIMER_LEFT_169_PENALTY=1.566355 PRIMER_RIGHT_169_PENALTY=2.167720 PRIMER_LEFT_169_SEQUENCE=ATGCCCTTCTCGTTCCTGAC PRIMER_RIGHT_169_SEQUENCE=ACGGTGTGCCAGTGAAGATG PRIMER_LEFT_169=573,20 PRIMER_RIGHT_169=657,20 PRIMER_LEFT_169_TM=61.566 PRIMER_RIGHT_169_TM=62.168 PRIMER_LEFT_169_GC_PERCENT=55.000 PRIMER_RIGHT_169_GC_PERCENT=55.000 PRIMER_LEFT_169_SELF_ANY=2.00 PRIMER_RIGHT_169_SELF_ANY=3.00 PRIMER_LEFT_169_SELF_END=1.00 PRIMER_RIGHT_169_SELF_END=0.00 PRIMER_LEFT_169_END_STABILITY=6.4000 PRIMER_RIGHT_169_END_STABILITY=6.6000 PRIMER_PAIR_169_COMPL_ANY=4.00 PRIMER_PAIR_169_COMPL_END=3.00 PRIMER_PAIR_169_PRODUCT_SIZE=85 PRIMER_PAIR_170_PENALTY=3.741350 PRIMER_LEFT_170_PENALTY=0.663548 PRIMER_RIGHT_170_PENALTY=3.077802 PRIMER_LEFT_170_SEQUENCE=CAGAATGGGAAGTGGGATTC PRIMER_RIGHT_170_SEQUENCE=GTTCAGGGTAGACTTCTTGGC PRIMER_LEFT_170=341,20 PRIMER_RIGHT_170=437,21 PRIMER_LEFT_170_TM=59.336 PRIMER_RIGHT_170_TM=57.922 PRIMER_LEFT_170_GC_PERCENT=50.000 PRIMER_RIGHT_170_GC_PERCENT=52.381 PRIMER_LEFT_170_SELF_ANY=4.00 PRIMER_RIGHT_170_SELF_ANY=4.00 PRIMER_LEFT_170_SELF_END=3.00 PRIMER_RIGHT_170_SELF_END=2.00 PRIMER_LEFT_170_END_STABILITY=6.6000 PRIMER_RIGHT_170_END_STABILITY=10.0000 PRIMER_PAIR_170_COMPL_ANY=5.00 PRIMER_PAIR_170_COMPL_END=0.00 PRIMER_PAIR_170_PRODUCT_SIZE=97 PRIMER_PAIR_171_PENALTY=3.741976 PRIMER_LEFT_171_PENALTY=2.137489 PRIMER_RIGHT_171_PENALTY=1.604487 PRIMER_LEFT_171_SEQUENCE=AGGTACCCACTGATGGTAATGC PRIMER_RIGHT_171_SEQUENCE=ACTTCCCATTCTGGACATTCA PRIMER_LEFT_171=255,22 PRIMER_RIGHT_171=353,21 PRIMER_LEFT_171_TM=60.137 PRIMER_RIGHT_171_TM=59.396 PRIMER_LEFT_171_GC_PERCENT=50.000 PRIMER_RIGHT_171_GC_PERCENT=42.857 PRIMER_LEFT_171_SELF_ANY=6.00 PRIMER_RIGHT_171_SELF_ANY=3.00 PRIMER_LEFT_171_SELF_END=2.00 PRIMER_RIGHT_171_SELF_END=2.00 PRIMER_LEFT_171_END_STABILITY=8.4000 PRIMER_RIGHT_171_END_STABILITY=6.9000 PRIMER_PAIR_171_COMPL_ANY=5.00 PRIMER_PAIR_171_COMPL_END=2.00 PRIMER_PAIR_171_PRODUCT_SIZE=99 PRIMER_PAIR_172_PENALTY=3.751205 PRIMER_LEFT_172_PENALTY=2.563176 PRIMER_RIGHT_172_PENALTY=1.188029 PRIMER_LEFT_172_SEQUENCE=GTTTGGCACTGCTCCTGCT PRIMER_RIGHT_172_SEQUENCE=AACATGGCAATCTGGGGTT PRIMER_LEFT_172=210,19 PRIMER_RIGHT_172=309,19 PRIMER_LEFT_172_TM=61.563 PRIMER_RIGHT_172_TM=60.188 PRIMER_LEFT_172_GC_PERCENT=57.895 PRIMER_RIGHT_172_GC_PERCENT=47.368 PRIMER_LEFT_172_SELF_ANY=5.00 PRIMER_RIGHT_172_SELF_ANY=4.00 PRIMER_LEFT_172_SELF_END=0.00 PRIMER_RIGHT_172_SELF_END=1.00 PRIMER_LEFT_172_END_STABILITY=8.2000 PRIMER_RIGHT_172_END_STABILITY=9.4000 PRIMER_PAIR_172_COMPL_ANY=4.00 PRIMER_PAIR_172_COMPL_END=0.00 PRIMER_PAIR_172_PRODUCT_SIZE=100 PRIMER_PAIR_173_PENALTY=3.754500 PRIMER_LEFT_173_PENALTY=2.150273 PRIMER_RIGHT_173_PENALTY=1.604227 PRIMER_LEFT_173_SEQUENCE=CCCCAGATTGCCATGTTC PRIMER_RIGHT_173_SEQUENCE=CCCTGATGGATCTGAATCCC PRIMER_LEFT_173=293,18 PRIMER_RIGHT_173=373,20 PRIMER_LEFT_173_TM=59.850 PRIMER_RIGHT_173_TM=61.604 PRIMER_LEFT_173_GC_PERCENT=55.556 PRIMER_RIGHT_173_GC_PERCENT=55.000 PRIMER_LEFT_173_SELF_ANY=4.00 PRIMER_RIGHT_173_SELF_ANY=5.00 PRIMER_LEFT_173_SELF_END=0.00 PRIMER_RIGHT_173_SELF_END=3.00 PRIMER_LEFT_173_END_STABILITY=6.7000 PRIMER_RIGHT_173_END_STABILITY=9.3000 PRIMER_PAIR_173_COMPL_ANY=5.00 PRIMER_PAIR_173_COMPL_END=0.00 PRIMER_PAIR_173_PRODUCT_SIZE=81 PRIMER_PAIR_174_PENALTY=3.758018 PRIMER_LEFT_174_PENALTY=2.402111 PRIMER_RIGHT_174_PENALTY=1.355907 PRIMER_LEFT_174_SEQUENCE=ACCGTCGCCAAAGAGACA PRIMER_RIGHT_174_SEQUENCE=CAAACTCTACCCCTCGGAAC PRIMER_LEFT_174=653,18 PRIMER_RIGHT_174=752,20 PRIMER_LEFT_174_TM=60.402 PRIMER_RIGHT_174_TM=58.644 PRIMER_LEFT_174_GC_PERCENT=55.556 PRIMER_RIGHT_174_GC_PERCENT=55.000 PRIMER_LEFT_174_SELF_ANY=3.00 PRIMER_RIGHT_174_SELF_ANY=3.00 PRIMER_LEFT_174_SELF_END=0.00 PRIMER_RIGHT_174_SELF_END=0.00 PRIMER_LEFT_174_END_STABILITY=6.4000 PRIMER_RIGHT_174_END_STABILITY=7.9000 PRIMER_PAIR_174_COMPL_ANY=4.00 PRIMER_PAIR_174_COMPL_END=0.00 PRIMER_PAIR_174_PRODUCT_SIZE=100 PRIMER_PAIR_175_PENALTY=3.764855 PRIMER_LEFT_175_PENALTY=2.063598 PRIMER_RIGHT_175_PENALTY=1.701257 PRIMER_LEFT_175_SEQUENCE=GCCAACCAACCAGTGACC PRIMER_RIGHT_175_SEQUENCE=AACTAAGCAGCGGTAGGGAAT PRIMER_LEFT_175=461,18 PRIMER_RIGHT_175=556,21 PRIMER_LEFT_175_TM=59.936 PRIMER_RIGHT_175_TM=59.299 PRIMER_LEFT_175_GC_PERCENT=61.111 PRIMER_RIGHT_175_GC_PERCENT=47.619 PRIMER_LEFT_175_SELF_ANY=3.00 PRIMER_RIGHT_175_SELF_ANY=3.00 PRIMER_LEFT_175_SELF_END=1.00 PRIMER_RIGHT_175_SELF_END=2.00 PRIMER_LEFT_175_END_STABILITY=7.9000 PRIMER_RIGHT_175_END_STABILITY=8.1000 PRIMER_PAIR_175_COMPL_ANY=4.00 PRIMER_PAIR_175_COMPL_END=0.00 PRIMER_PAIR_175_PRODUCT_SIZE=96 PRIMER_PAIR_176_PENALTY=3.771832 PRIMER_LEFT_176_PENALTY=1.749643 PRIMER_RIGHT_176_PENALTY=2.022189 PRIMER_LEFT_176_SEQUENCE=TGATTCCCTACCGCTGCTT PRIMER_RIGHT_176_SEQUENCE=CATCCATCCTCTCCTGGTG PRIMER_LEFT_176=534,19 PRIMER_RIGHT_176=626,19 PRIMER_LEFT_176_TM=60.750 PRIMER_RIGHT_176_TM=58.978 PRIMER_LEFT_176_GC_PERCENT=52.632 PRIMER_RIGHT_176_GC_PERCENT=57.895 PRIMER_LEFT_176_SELF_ANY=3.00 PRIMER_RIGHT_176_SELF_ANY=3.00 PRIMER_LEFT_176_SELF_END=0.00 PRIMER_RIGHT_176_SELF_END=3.00 PRIMER_LEFT_176_END_STABILITY=8.5000 PRIMER_RIGHT_176_END_STABILITY=8.2000 PRIMER_PAIR_176_COMPL_ANY=3.00 PRIMER_PAIR_176_COMPL_END=0.00 PRIMER_PAIR_176_PRODUCT_SIZE=93 PRIMER_PAIR_177_PENALTY=3.787393 PRIMER_LEFT_177_PENALTY=2.431486 PRIMER_RIGHT_177_PENALTY=1.355907 PRIMER_LEFT_177_SEQUENCE=CCAAAGAGACATGCAGTGAGAA PRIMER_RIGHT_177_SEQUENCE=ACAAACTCTACCCCTCGGAA PRIMER_LEFT_177=660,22 PRIMER_RIGHT_177=753,20 PRIMER_LEFT_177_TM=60.431 PRIMER_RIGHT_177_TM=58.644 PRIMER_LEFT_177_GC_PERCENT=45.455 PRIMER_RIGHT_177_GC_PERCENT=50.000 PRIMER_LEFT_177_SELF_ANY=4.00 PRIMER_RIGHT_177_SELF_ANY=3.00 PRIMER_LEFT_177_SELF_END=0.00 PRIMER_RIGHT_177_SELF_END=1.00 PRIMER_LEFT_177_END_STABILITY=6.7000 PRIMER_RIGHT_177_END_STABILITY=10.2000 PRIMER_PAIR_177_COMPL_ANY=4.00 PRIMER_PAIR_177_COMPL_END=0.00 PRIMER_PAIR_177_PRODUCT_SIZE=94 PRIMER_PAIR_178_PENALTY=3.794945 PRIMER_LEFT_178_PENALTY=2.485003 PRIMER_RIGHT_178_PENALTY=1.309943 PRIMER_LEFT_178_SEQUENCE=TACCCACTGATGGTAATGCTG PRIMER_RIGHT_178_SEQUENCE=CCCACTTCCCATTCTGGAC PRIMER_LEFT_178=258,21 PRIMER_RIGHT_178=356,19 PRIMER_LEFT_178_TM=58.515 PRIMER_RIGHT_178_TM=60.310 PRIMER_LEFT_178_GC_PERCENT=47.619 PRIMER_RIGHT_178_GC_PERCENT=57.895 PRIMER_LEFT_178_SELF_ANY=5.00 PRIMER_RIGHT_178_SELF_ANY=3.00 PRIMER_LEFT_178_SELF_END=1.00 PRIMER_RIGHT_178_SELF_END=1.00 PRIMER_LEFT_178_END_STABILITY=8.5000 PRIMER_RIGHT_178_END_STABILITY=7.9000 PRIMER_PAIR_178_COMPL_ANY=5.00 PRIMER_PAIR_178_COMPL_END=1.00 PRIMER_PAIR_178_PRODUCT_SIZE=99 PRIMER_PAIR_179_PENALTY=3.798463 PRIMER_LEFT_179_PENALTY=2.045801 PRIMER_RIGHT_179_PENALTY=1.752662 PRIMER_LEFT_179_SEQUENCE=CAGTGCAAGACCCATCCC PRIMER_RIGHT_179_SEQUENCE=AGGAACGAGAAGGGCATCA PRIMER_LEFT_179=509,18 PRIMER_RIGHT_179=589,19 PRIMER_LEFT_179_TM=60.046 PRIMER_RIGHT_179_TM=60.753 PRIMER_LEFT_179_GC_PERCENT=61.111 PRIMER_RIGHT_179_GC_PERCENT=52.632 PRIMER_LEFT_179_SELF_ANY=4.00 PRIMER_RIGHT_179_SELF_ANY=2.00 PRIMER_LEFT_179_SELF_END=0.00 PRIMER_RIGHT_179_SELF_END=1.00 PRIMER_LEFT_179_END_STABILITY=9.3000 PRIMER_RIGHT_179_END_STABILITY=6.9000 PRIMER_PAIR_179_COMPL_ANY=4.00 PRIMER_PAIR_179_COMPL_END=3.00 PRIMER_PAIR_179_PRODUCT_SIZE=81 PRIMER_PAIR_180_PENALTY=3.810679 PRIMER_LEFT_180_PENALTY=2.027792 PRIMER_RIGHT_180_PENALTY=1.782887 PRIMER_LEFT_180_SEQUENCE=ATGTGGTAGAAGCCAACCAAC PRIMER_RIGHT_180_SEQUENCE=ACAAAGTGGGGATGGGTCT PRIMER_LEFT_180=450,21 PRIMER_RIGHT_180=534,19 PRIMER_LEFT_180_TM=58.972 PRIMER_RIGHT_180_TM=59.217 PRIMER_LEFT_180_GC_PERCENT=47.619 PRIMER_RIGHT_180_GC_PERCENT=52.632 PRIMER_LEFT_180_SELF_ANY=4.00 PRIMER_RIGHT_180_SELF_ANY=2.00 PRIMER_LEFT_180_SELF_END=2.00 PRIMER_RIGHT_180_SELF_END=0.00 PRIMER_LEFT_180_END_STABILITY=8.2000 PRIMER_RIGHT_180_END_STABILITY=7.6000 PRIMER_PAIR_180_COMPL_ANY=4.00 PRIMER_PAIR_180_COMPL_END=3.00 PRIMER_PAIR_180_PRODUCT_SIZE=85 PRIMER_PAIR_181_PENALTY=3.813241 PRIMER_LEFT_181_PENALTY=2.246886 PRIMER_RIGHT_181_PENALTY=1.566355 PRIMER_LEFT_181_SEQUENCE=AGCAGTGCAAGACCCATCC PRIMER_RIGHT_181_SEQUENCE=AGGAACGAGAAGGGCATCAC PRIMER_LEFT_181=507,19 PRIMER_RIGHT_181=589,20 PRIMER_LEFT_181_TM=61.247 PRIMER_RIGHT_181_TM=61.566 PRIMER_LEFT_181_GC_PERCENT=57.895 PRIMER_RIGHT_181_GC_PERCENT=55.000 PRIMER_LEFT_181_SELF_ANY=5.00 PRIMER_RIGHT_181_SELF_ANY=2.00 PRIMER_LEFT_181_SELF_END=0.00 PRIMER_RIGHT_181_SELF_END=2.00 PRIMER_LEFT_181_END_STABILITY=8.1000 PRIMER_RIGHT_181_END_STABILITY=6.3000 PRIMER_PAIR_181_COMPL_ANY=4.00 PRIMER_PAIR_181_COMPL_END=3.00 PRIMER_PAIR_181_PRODUCT_SIZE=83 PRIMER_PAIR_182_PENALTY=3.830330 PRIMER_LEFT_182_PENALTY=1.852336 PRIMER_RIGHT_182_PENALTY=1.977994 PRIMER_LEFT_182_SEQUENCE=TCTTACACCAGGAGAGGATGG PRIMER_RIGHT_182_SEQUENCE=TCTCACTGCATGTCTCTTTGG PRIMER_LEFT_182=603,21 PRIMER_RIGHT_182=680,21 PRIMER_LEFT_182_TM=59.148 PRIMER_RIGHT_182_TM=59.022 PRIMER_LEFT_182_GC_PERCENT=52.381 PRIMER_RIGHT_182_GC_PERCENT=47.619 PRIMER_LEFT_182_SELF_ANY=3.00 PRIMER_RIGHT_182_SELF_ANY=4.00 PRIMER_LEFT_182_SELF_END=0.00 PRIMER_RIGHT_182_SELF_END=0.00 PRIMER_LEFT_182_END_STABILITY=8.1000 PRIMER_RIGHT_182_END_STABILITY=8.8000 PRIMER_PAIR_182_COMPL_ANY=5.00 PRIMER_PAIR_182_COMPL_END=3.00 PRIMER_PAIR_182_PRODUCT_SIZE=78 PRIMER_PAIR_183_PENALTY=3.831476 PRIMER_LEFT_183_PENALTY=1.907724 PRIMER_RIGHT_183_PENALTY=1.923751 PRIMER_LEFT_183_SEQUENCE=CAGGGACCAAAACCTGCAT PRIMER_RIGHT_183_SEQUENCE=TGGTTGGCTTCTACCACATT PRIMER_LEFT_183=369,19 PRIMER_RIGHT_183=468,20 PRIMER_LEFT_183_TM=60.908 PRIMER_RIGHT_183_TM=58.076 PRIMER_LEFT_183_GC_PERCENT=52.632 PRIMER_RIGHT_183_GC_PERCENT=45.000 PRIMER_LEFT_183_SELF_ANY=4.00 PRIMER_RIGHT_183_SELF_ANY=4.00 PRIMER_LEFT_183_SELF_END=2.00 PRIMER_RIGHT_183_SELF_END=1.00 PRIMER_LEFT_183_END_STABILITY=8.4000 PRIMER_RIGHT_183_END_STABILITY=6.6000 PRIMER_PAIR_183_COMPL_ANY=4.00 PRIMER_PAIR_183_COMPL_END=1.00 PRIMER_PAIR_183_PRODUCT_SIZE=100 PRIMER_PAIR_184_PENALTY=3.834074 PRIMER_LEFT_184_PENALTY=2.203644 PRIMER_RIGHT_184_PENALTY=1.630431 PRIMER_LEFT_184_SEQUENCE=CTGGAGGTACCCACTGATGGT PRIMER_RIGHT_184_SEQUENCE=GTGCATGTTCAGTCTGCCA PRIMER_LEFT_184=251,21 PRIMER_RIGHT_184=331,19 PRIMER_LEFT_184_TM=61.204 PRIMER_RIGHT_184_TM=59.370 PRIMER_LEFT_184_GC_PERCENT=57.143 PRIMER_RIGHT_184_GC_PERCENT=52.632 PRIMER_LEFT_184_SELF_ANY=6.00 PRIMER_RIGHT_184_SELF_ANY=5.00 PRIMER_LEFT_184_SELF_END=3.00 PRIMER_RIGHT_184_SELF_END=3.00 PRIMER_LEFT_184_END_STABILITY=7.8000 PRIMER_RIGHT_184_END_STABILITY=10.0000 PRIMER_PAIR_184_COMPL_ANY=5.00 PRIMER_PAIR_184_COMPL_END=2.00 PRIMER_PAIR_184_PRODUCT_SIZE=81 PRIMER_PAIR_185_PENALTY=3.841761 PRIMER_LEFT_185_PENALTY=1.566355 PRIMER_RIGHT_185_PENALTY=2.275406 PRIMER_LEFT_185_SEQUENCE=GTGATGCCCTTCTCGTTCCT PRIMER_RIGHT_185_SEQUENCE=GTCTCTTTGGCGACGGTGT PRIMER_LEFT_185=570,20 PRIMER_RIGHT_185=669,19 PRIMER_LEFT_185_TM=61.566 PRIMER_RIGHT_185_TM=61.275 PRIMER_LEFT_185_GC_PERCENT=55.000 PRIMER_RIGHT_185_GC_PERCENT=57.895 PRIMER_LEFT_185_SELF_ANY=2.00 PRIMER_RIGHT_185_SELF_ANY=3.00 PRIMER_LEFT_185_SELF_END=0.00 PRIMER_RIGHT_185_SELF_END=1.00 PRIMER_LEFT_185_END_STABILITY=8.2000 PRIMER_RIGHT_185_END_STABILITY=7.6000 PRIMER_PAIR_185_COMPL_ANY=3.00 PRIMER_PAIR_185_COMPL_END=0.00 PRIMER_PAIR_185_PRODUCT_SIZE=100 PRIMER_PAIR_186_PENALTY=3.843348 PRIMER_LEFT_186_PENALTY=1.852336 PRIMER_RIGHT_186_PENALTY=1.991012 PRIMER_LEFT_186_SEQUENCE=CTTACACCAGGAGAGGATGGA PRIMER_RIGHT_186_SEQUENCE=CTCACTGCATGTCTCTTTGGC PRIMER_LEFT_186=604,21 PRIMER_RIGHT_186=679,21 PRIMER_LEFT_186_TM=59.148 PRIMER_RIGHT_186_TM=60.991 PRIMER_LEFT_186_GC_PERCENT=52.381 PRIMER_RIGHT_186_GC_PERCENT=52.381 PRIMER_LEFT_186_SELF_ANY=3.00 PRIMER_RIGHT_186_SELF_ANY=4.00 PRIMER_LEFT_186_SELF_END=0.00 PRIMER_RIGHT_186_SELF_END=2.00 PRIMER_LEFT_186_END_STABILITY=8.1000 PRIMER_RIGHT_186_END_STABILITY=10.0000 PRIMER_PAIR_186_COMPL_ANY=4.00 PRIMER_PAIR_186_COMPL_END=3.00 PRIMER_PAIR_186_PRODUCT_SIZE=76 PRIMER_PAIR_187_PENALTY=3.847029 PRIMER_LEFT_187_PENALTY=2.631568 PRIMER_RIGHT_187_PENALTY=1.215461 PRIMER_LEFT_187_SEQUENCE=ATCCTGCAGTATTGCCAAGAAG PRIMER_RIGHT_187_SEQUENCE=TGGATGGTCACTGGTTGGT PRIMER_LEFT_187=404,22 PRIMER_RIGHT_187=483,19 PRIMER_LEFT_187_TM=60.632 PRIMER_RIGHT_187_TM=59.785 PRIMER_LEFT_187_GC_PERCENT=45.455 PRIMER_RIGHT_187_GC_PERCENT=52.632 PRIMER_LEFT_187_SELF_ANY=8.00 PRIMER_RIGHT_187_SELF_ANY=3.00 PRIMER_LEFT_187_SELF_END=2.00 PRIMER_RIGHT_187_SELF_END=0.00 PRIMER_LEFT_187_END_STABILITY=6.7000 PRIMER_RIGHT_187_END_STABILITY=8.2000 PRIMER_PAIR_187_COMPL_ANY=4.00 PRIMER_PAIR_187_COMPL_END=3.00 PRIMER_PAIR_187_PRODUCT_SIZE=80 PRIMER_PAIR_188_PENALTY=3.852497 PRIMER_LEFT_188_PENALTY=2.222066 PRIMER_RIGHT_188_PENALTY=1.630431 PRIMER_LEFT_188_SEQUENCE=GGAGGTACCCACTGATGGTAA PRIMER_RIGHT_188_SEQUENCE=TGTGCATGTTCAGTCTGCC PRIMER_LEFT_188=253,21 PRIMER_RIGHT_188=332,19 PRIMER_LEFT_188_TM=58.778 PRIMER_RIGHT_188_TM=59.370 PRIMER_LEFT_188_GC_PERCENT=52.381 PRIMER_RIGHT_188_GC_PERCENT=52.632 PRIMER_LEFT_188_SELF_ANY=6.00 PRIMER_RIGHT_188_SELF_ANY=5.00 PRIMER_LEFT_188_SELF_END=3.00 PRIMER_RIGHT_188_SELF_END=1.00 PRIMER_LEFT_188_END_STABILITY=7.2000 PRIMER_RIGHT_188_END_STABILITY=9.7000 PRIMER_PAIR_188_COMPL_ANY=5.00 PRIMER_PAIR_188_COMPL_END=1.00 PRIMER_PAIR_188_PRODUCT_SIZE=80 PRIMER_PAIR_189_PENALTY=3.857288 PRIMER_LEFT_189_PENALTY=1.512710 PRIMER_RIGHT_189_PENALTY=2.344578 PRIMER_LEFT_189_SEQUENCE=TGAATGTCCAGAATGGGAAG PRIMER_RIGHT_189_SEQUENCE=CAGGATGCCTTCCTTGGTA PRIMER_LEFT_189=333,20 PRIMER_RIGHT_189=409,19 PRIMER_LEFT_189_TM=58.487 PRIMER_RIGHT_189_TM=58.655 PRIMER_LEFT_189_GC_PERCENT=45.000 PRIMER_RIGHT_189_GC_PERCENT=52.632 PRIMER_LEFT_189_SELF_ANY=3.00 PRIMER_RIGHT_189_SELF_ANY=7.00 PRIMER_LEFT_189_SELF_END=0.00 PRIMER_RIGHT_189_SELF_END=2.00 PRIMER_LEFT_189_END_STABILITY=8.2000 PRIMER_RIGHT_189_END_STABILITY=7.2000 PRIMER_PAIR_189_COMPL_ANY=5.00 PRIMER_PAIR_189_COMPL_END=1.00 PRIMER_PAIR_189_PRODUCT_SIZE=77 PRIMER_PAIR_190_PENALTY=3.865253 PRIMER_LEFT_190_PENALTY=1.566355 PRIMER_RIGHT_190_PENALTY=2.298898 PRIMER_LEFT_190_SEQUENCE=AGTGATGCCCTTCTCGTTCC PRIMER_RIGHT_190_SEQUENCE=TGTGCCAGTGAAGATGAGTTTC PRIMER_LEFT_190=569,20 PRIMER_RIGHT_190=653,22 PRIMER_LEFT_190_TM=61.566 PRIMER_RIGHT_190_TM=60.299 PRIMER_LEFT_190_GC_PERCENT=55.000 PRIMER_RIGHT_190_GC_PERCENT=45.455 PRIMER_LEFT_190_SELF_ANY=2.00 PRIMER_RIGHT_190_SELF_ANY=3.00 PRIMER_LEFT_190_SELF_END=0.00 PRIMER_RIGHT_190_SELF_END=3.00 PRIMER_LEFT_190_END_STABILITY=7.9000 PRIMER_RIGHT_190_END_STABILITY=6.7000 PRIMER_PAIR_190_COMPL_ANY=4.00 PRIMER_PAIR_190_COMPL_END=0.00 PRIMER_PAIR_190_PRODUCT_SIZE=85 PRIMER_PAIR_191_PENALTY=3.880111 PRIMER_LEFT_191_PENALTY=2.127449 PRIMER_RIGHT_191_PENALTY=1.752662 PRIMER_LEFT_191_SEQUENCE=AGACCCATCCCCACTTTGTG PRIMER_RIGHT_191_SEQUENCE=CAGGAACGAGAAGGGCATC PRIMER_LEFT_191=516,20 PRIMER_RIGHT_191=590,19 PRIMER_LEFT_191_TM=62.127 PRIMER_RIGHT_191_TM=60.753 PRIMER_LEFT_191_GC_PERCENT=55.000 PRIMER_RIGHT_191_GC_PERCENT=57.895 PRIMER_LEFT_191_SELF_ANY=3.00 PRIMER_RIGHT_191_SELF_ANY=2.00 PRIMER_LEFT_191_SELF_END=3.00 PRIMER_RIGHT_191_SELF_END=2.00 PRIMER_LEFT_191_END_STABILITY=7.0000 PRIMER_RIGHT_191_END_STABILITY=8.1000 PRIMER_PAIR_191_COMPL_ANY=4.00 PRIMER_PAIR_191_COMPL_END=1.00 PRIMER_PAIR_191_PRODUCT_SIZE=75 PRIMER_PAIR_192_PENALTY=3.893190 PRIMER_LEFT_192_PENALTY=1.557214 PRIMER_RIGHT_192_PENALTY=2.335976 PRIMER_LEFT_192_SEQUENCE=GCGAAACTCATCTTCACTGG PRIMER_RIGHT_192_SEQUENCE=ACATGCCGTAGTCATGCAA PRIMER_LEFT_192=630,20 PRIMER_RIGHT_192=710,19 PRIMER_LEFT_192_TM=58.443 PRIMER_RIGHT_192_TM=58.664 PRIMER_LEFT_192_GC_PERCENT=50.000 PRIMER_RIGHT_192_GC_PERCENT=47.368 PRIMER_LEFT_192_SELF_ANY=3.00 PRIMER_RIGHT_192_SELF_ANY=6.00 PRIMER_LEFT_192_SELF_END=1.00 PRIMER_RIGHT_192_SELF_END=2.00 PRIMER_LEFT_192_END_STABILITY=7.9000 PRIMER_RIGHT_192_END_STABILITY=8.8000 PRIMER_PAIR_192_COMPL_ANY=3.00 PRIMER_PAIR_192_COMPL_END=1.00 PRIMER_PAIR_192_PRODUCT_SIZE=81 PRIMER_PAIR_193_PENALTY=3.905473 PRIMER_LEFT_193_PENALTY=1.532916 PRIMER_RIGHT_193_PENALTY=2.372557 PRIMER_LEFT_193_SEQUENCE=TGCACATGAATGTCCAGAATG PRIMER_RIGHT_193_SEQUENCE=CTTCCTTGGTATCAATGCAGGT PRIMER_LEFT_193=327,21 PRIMER_RIGHT_193=401,22 PRIMER_LEFT_193_TM=60.533 PRIMER_RIGHT_193_TM=60.373 PRIMER_LEFT_193_GC_PERCENT=42.857 PRIMER_RIGHT_193_GC_PERCENT=45.455 PRIMER_LEFT_193_SELF_ANY=8.00 PRIMER_RIGHT_193_SELF_ANY=8.00 PRIMER_LEFT_193_SELF_END=1.00 PRIMER_RIGHT_193_SELF_END=2.00 PRIMER_LEFT_193_END_STABILITY=6.9000 PRIMER_RIGHT_193_END_STABILITY=7.9000 PRIMER_PAIR_193_COMPL_ANY=4.00 PRIMER_PAIR_193_COMPL_END=3.00 PRIMER_PAIR_193_PRODUCT_SIZE=75 PRIMER_PAIR_194_PENALTY=3.906167 PRIMER_LEFT_194_PENALTY=0.828193 PRIMER_RIGHT_194_PENALTY=3.077974 PRIMER_LEFT_194_SEQUENCE=TGGGATTCAGATCCATCAGG PRIMER_RIGHT_194_SEQUENCE=AGTTCAGGGTAGACTTCTTGGC PRIMER_LEFT_194=353,20 PRIMER_RIGHT_194=438,22 PRIMER_LEFT_194_TM=60.828 PRIMER_RIGHT_194_TM=58.922 PRIMER_LEFT_194_GC_PERCENT=50.000 PRIMER_RIGHT_194_GC_PERCENT=50.000 PRIMER_LEFT_194_SELF_ANY=5.00 PRIMER_RIGHT_194_SELF_ANY=4.00 PRIMER_LEFT_194_SELF_END=2.00 PRIMER_RIGHT_194_SELF_END=2.00 PRIMER_LEFT_194_END_STABILITY=8.2000 PRIMER_RIGHT_194_END_STABILITY=10.0000 PRIMER_PAIR_194_COMPL_ANY=3.00 PRIMER_PAIR_194_COMPL_END=1.00 PRIMER_PAIR_194_PRODUCT_SIZE=86 PRIMER_PAIR_195_PENALTY=3.919309 PRIMER_LEFT_195_PENALTY=2.692052 PRIMER_RIGHT_195_PENALTY=1.227257 PRIMER_LEFT_195_SEQUENCE=CATTGATACCAAGGAAGGCATC PRIMER_RIGHT_195_SEQUENCE=CACTGGTTGGTTGGCTTCTA PRIMER_LEFT_195=385,22 PRIMER_RIGHT_195=475,20 PRIMER_LEFT_195_TM=60.692 PRIMER_RIGHT_195_TM=58.773 PRIMER_LEFT_195_GC_PERCENT=45.455 PRIMER_RIGHT_195_GC_PERCENT=50.000 PRIMER_LEFT_195_SELF_ANY=6.00 PRIMER_RIGHT_195_SELF_ANY=3.00 PRIMER_LEFT_195_SELF_END=3.00 PRIMER_RIGHT_195_SELF_END=2.00 PRIMER_LEFT_195_END_STABILITY=8.1000 PRIMER_RIGHT_195_END_STABILITY=6.0000 PRIMER_PAIR_195_COMPL_ANY=5.00 PRIMER_PAIR_195_COMPL_END=3.00 PRIMER_PAIR_195_PRODUCT_SIZE=91 PRIMER_PAIR_196_PENALTY=3.930760 PRIMER_LEFT_196_PENALTY=2.027792 PRIMER_RIGHT_196_PENALTY=1.902968 PRIMER_LEFT_196_SEQUENCE=ACCAATGTGGTAGAAGCCAAC PRIMER_RIGHT_196_SEQUENCE=AATCACAAAGTGGGGATGGG PRIMER_LEFT_196=446,21 PRIMER_RIGHT_196=538,20 PRIMER_LEFT_196_TM=58.972 PRIMER_RIGHT_196_TM=61.903 PRIMER_LEFT_196_GC_PERCENT=47.619 PRIMER_RIGHT_196_GC_PERCENT=50.000 PRIMER_LEFT_196_SELF_ANY=6.00 PRIMER_RIGHT_196_SELF_ANY=4.00 PRIMER_LEFT_196_SELF_END=0.00 PRIMER_RIGHT_196_SELF_END=0.00 PRIMER_LEFT_196_END_STABILITY=8.2000 PRIMER_RIGHT_196_END_STABILITY=9.6000 PRIMER_PAIR_196_COMPL_ANY=4.00 PRIMER_PAIR_196_COMPL_END=2.00 PRIMER_PAIR_196_PRODUCT_SIZE=93 PRIMER_PAIR_197_PENALTY=3.930997 PRIMER_LEFT_197_PENALTY=2.618102 PRIMER_RIGHT_197_PENALTY=1.312895 PRIMER_LEFT_197_SEQUENCE=CTGAACCCCAGATTGCCA PRIMER_RIGHT_197_SEQUENCE=CTGAATCCCACTTCCCATTCT PRIMER_LEFT_197=288,18 PRIMER_RIGHT_197=362,21 PRIMER_LEFT_197_TM=60.618 PRIMER_RIGHT_197_TM=60.313 PRIMER_LEFT_197_GC_PERCENT=55.556 PRIMER_RIGHT_197_GC_PERCENT=47.619 PRIMER_LEFT_197_SELF_ANY=3.00 PRIMER_RIGHT_197_SELF_ANY=4.00 PRIMER_LEFT_197_SELF_END=3.00 PRIMER_RIGHT_197_SELF_END=0.00 PRIMER_LEFT_197_END_STABILITY=10.0000 PRIMER_RIGHT_197_END_STABILITY=6.6000 PRIMER_PAIR_197_COMPL_ANY=4.00 PRIMER_PAIR_197_COMPL_END=3.00 PRIMER_PAIR_197_PRODUCT_SIZE=75 PRIMER_PAIR_198_PENALTY=3.933587 PRIMER_LEFT_198_PENALTY=1.883110 PRIMER_RIGHT_198_PENALTY=2.050476 PRIMER_LEFT_198_SEQUENCE=ATCTTCACTGGCACACCGT PRIMER_RIGHT_198_SEQUENCE=ACTTGTCAATTCCGCAGGG PRIMER_LEFT_198=639,19 PRIMER_RIGHT_198=734,19 PRIMER_LEFT_198_TM=59.117 PRIMER_RIGHT_198_TM=61.050 PRIMER_LEFT_198_GC_PERCENT=52.632 PRIMER_RIGHT_198_GC_PERCENT=52.632 PRIMER_LEFT_198_SELF_ANY=3.00 PRIMER_RIGHT_198_SELF_ANY=5.00 PRIMER_LEFT_198_SELF_END=3.00 PRIMER_RIGHT_198_SELF_END=1.00 PRIMER_LEFT_198_END_STABILITY=9.3000 PRIMER_RIGHT_198_END_STABILITY=9.7000 PRIMER_PAIR_198_COMPL_ANY=3.00 PRIMER_PAIR_198_COMPL_END=2.00 PRIMER_PAIR_198_PRODUCT_SIZE=96 PRIMER_PAIR_199_PENALTY=3.944577 PRIMER_LEFT_199_PENALTY=1.616405 PRIMER_RIGHT_199_PENALTY=2.328173 PRIMER_LEFT_199_SEQUENCE=GTGATGCCCTTCTCGTTCC PRIMER_RIGHT_199_SEQUENCE=CGGTGTGCCAGTGAAGATG PRIMER_LEFT_199=570,19 PRIMER_RIGHT_199=656,19 PRIMER_LEFT_199_TM=60.616 PRIMER_RIGHT_199_TM=61.328 PRIMER_LEFT_199_GC_PERCENT=57.895 PRIMER_RIGHT_199_GC_PERCENT=57.895 PRIMER_LEFT_199_SELF_ANY=2.00 PRIMER_RIGHT_199_SELF_ANY=3.00 PRIMER_LEFT_199_SELF_END=0.00 PRIMER_RIGHT_199_SELF_END=0.00 PRIMER_LEFT_199_END_STABILITY=7.9000 PRIMER_RIGHT_199_END_STABILITY=6.6000 PRIMER_PAIR_199_COMPL_ANY=4.00 PRIMER_PAIR_199_COMPL_END=1.00 PRIMER_PAIR_199_PRODUCT_SIZE=87 PRIMER_PAIR_200_PENALTY=3.944972 PRIMER_LEFT_200_PENALTY=2.192310 PRIMER_RIGHT_200_PENALTY=1.752662 PRIMER_LEFT_200_SEQUENCE=GACCCATCCCCACTTTGTG PRIMER_RIGHT_200_SEQUENCE=TCAGGAACGAGAAGGGCAT PRIMER_LEFT_200=517,19 PRIMER_RIGHT_200=591,19 PRIMER_LEFT_200_TM=61.192 PRIMER_RIGHT_200_TM=60.753 PRIMER_LEFT_200_GC_PERCENT=57.895 PRIMER_RIGHT_200_GC_PERCENT=52.632 PRIMER_LEFT_200_SELF_ANY=3.00 PRIMER_RIGHT_200_SELF_ANY=2.00 PRIMER_LEFT_200_SELF_END=3.00 PRIMER_RIGHT_200_SELF_END=2.00 PRIMER_LEFT_200_END_STABILITY=7.0000 PRIMER_RIGHT_200_END_STABILITY=9.6000 PRIMER_PAIR_200_COMPL_ANY=4.00 PRIMER_PAIR_200_COMPL_END=2.00 PRIMER_PAIR_200_PRODUCT_SIZE=75 PRIMER_PAIR_201_PENALTY=3.953976 PRIMER_LEFT_201_PENALTY=2.018039 PRIMER_RIGHT_201_PENALTY=1.935937 PRIMER_LEFT_201_SEQUENCE=ATTCCCTACCGCTGCTTAGT PRIMER_RIGHT_201_SEQUENCE=TTTCGCAAACATCCATCCT PRIMER_LEFT_201=536,20 PRIMER_RIGHT_201=635,19 PRIMER_LEFT_201_TM=57.982 PRIMER_RIGHT_201_TM=59.064 PRIMER_LEFT_201_GC_PERCENT=50.000 PRIMER_RIGHT_201_GC_PERCENT=42.105 PRIMER_LEFT_201_SELF_ANY=3.00 PRIMER_RIGHT_201_SELF_ANY=4.00 PRIMER_LEFT_201_SELF_END=1.00 PRIMER_RIGHT_201_SELF_END=0.00 PRIMER_LEFT_201_END_STABILITY=5.7000 PRIMER_RIGHT_201_END_STABILITY=7.8000 PRIMER_PAIR_201_COMPL_ANY=3.00 PRIMER_PAIR_201_COMPL_END=1.00 PRIMER_PAIR_201_PRODUCT_SIZE=100 PRIMER_PAIR_202_PENALTY=3.965341 PRIMER_LEFT_202_PENALTY=1.752662 PRIMER_RIGHT_202_PENALTY=2.212679 PRIMER_LEFT_202_SEQUENCE=ATGCCCTTCTCGTTCCTGA PRIMER_RIGHT_202_SEQUENCE=ATGTCTCTTTGGCGACGG PRIMER_LEFT_202=573,19 PRIMER_RIGHT_202=671,18 PRIMER_LEFT_202_TM=60.753 PRIMER_RIGHT_202_TM=59.787 PRIMER_LEFT_202_GC_PERCENT=52.632 PRIMER_RIGHT_202_GC_PERCENT=55.556 PRIMER_LEFT_202_SELF_ANY=2.00 PRIMER_RIGHT_202_SELF_ANY=3.00 PRIMER_LEFT_202_SELF_END=2.00 PRIMER_RIGHT_202_SELF_END=1.00 PRIMER_LEFT_202_END_STABILITY=8.2000 PRIMER_RIGHT_202_END_STABILITY=9.6000 PRIMER_PAIR_202_COMPL_ANY=3.00 PRIMER_PAIR_202_COMPL_END=0.00 PRIMER_PAIR_202_PRODUCT_SIZE=99 PRIMER_PAIR_203_PENALTY=3.975396 PRIMER_LEFT_203_PENALTY=2.618102 PRIMER_RIGHT_203_PENALTY=1.357294 PRIMER_LEFT_203_SEQUENCE=TGGCTGAACCCCAGATTG PRIMER_RIGHT_203_SEQUENCE=ATCTGAATCCCACTTCCCATT PRIMER_LEFT_203=285,18 PRIMER_RIGHT_203=364,21 PRIMER_LEFT_203_TM=60.618 PRIMER_RIGHT_203_TM=59.643 PRIMER_LEFT_203_GC_PERCENT=55.556 PRIMER_RIGHT_203_GC_PERCENT=42.857 PRIMER_LEFT_203_SELF_ANY=3.00 PRIMER_RIGHT_203_SELF_ANY=3.00 PRIMER_LEFT_203_SELF_END=2.00 PRIMER_RIGHT_203_SELF_END=1.00 PRIMER_LEFT_203_END_STABILITY=6.9000 PRIMER_RIGHT_203_END_STABILITY=8.4000 PRIMER_PAIR_203_COMPL_ANY=5.00 PRIMER_PAIR_203_COMPL_END=2.00 PRIMER_PAIR_203_PRODUCT_SIZE=80 PRIMER_PAIR_204_PENALTY=3.977161 PRIMER_LEFT_204_PENALTY=2.420393 PRIMER_RIGHT_204_PENALTY=1.556768 PRIMER_LEFT_204_SEQUENCE=TGCCATGTTCTGTGGCAG PRIMER_RIGHT_204_SEQUENCE=TCCTTGGTATCAATGCAGGTT PRIMER_LEFT_204=301,18 PRIMER_RIGHT_204=399,21 PRIMER_LEFT_204_TM=60.420 PRIMER_RIGHT_204_TM=59.443 PRIMER_LEFT_204_GC_PERCENT=55.556 PRIMER_RIGHT_204_GC_PERCENT=42.857 PRIMER_LEFT_204_SELF_ANY=5.00 PRIMER_RIGHT_204_SELF_ANY=6.00 PRIMER_LEFT_204_SELF_END=3.00 PRIMER_RIGHT_204_SELF_END=0.00 PRIMER_LEFT_204_END_STABILITY=9.7000 PRIMER_RIGHT_204_END_STABILITY=7.9000 PRIMER_PAIR_204_COMPL_ANY=3.00 PRIMER_PAIR_204_COMPL_END=0.00 PRIMER_PAIR_204_PRODUCT_SIZE=99 PRIMER_PAIR_205_PENALTY=3.980046 PRIMER_LEFT_205_PENALTY=1.595257 PRIMER_RIGHT_205_PENALTY=2.384789 PRIMER_LEFT_205_SEQUENCE=CCCCACTTTGTGATTCCCTAC PRIMER_RIGHT_205_SEQUENCE=TTTGCACTTGTCAGGAACGAG PRIMER_LEFT_205=524,21 PRIMER_RIGHT_205=601,21 PRIMER_LEFT_205_TM=60.595 PRIMER_RIGHT_205_TM=61.385 PRIMER_LEFT_205_GC_PERCENT=52.381 PRIMER_RIGHT_205_GC_PERCENT=47.619 PRIMER_LEFT_205_SELF_ANY=3.00 PRIMER_RIGHT_205_SELF_ANY=4.00 PRIMER_LEFT_205_SELF_END=1.00 PRIMER_RIGHT_205_SELF_END=2.00 PRIMER_LEFT_205_END_STABILITY=6.9000 PRIMER_RIGHT_205_END_STABILITY=8.1000 PRIMER_PAIR_205_COMPL_ANY=4.00 PRIMER_PAIR_205_COMPL_END=2.00 PRIMER_PAIR_205_PRODUCT_SIZE=78 PRIMER_PAIR_206_PENALTY=3.995346 PRIMER_LEFT_206_PENALTY=2.402111 PRIMER_RIGHT_206_PENALTY=1.593235 PRIMER_LEFT_206_SEQUENCE=GGTTTGGCACTGCTCCTG PRIMER_RIGHT_206_SEQUENCE=CAGGCCAGCATTACCATCAG PRIMER_LEFT_206=209,18 PRIMER_RIGHT_206=283,20 PRIMER_LEFT_206_TM=60.402 PRIMER_RIGHT_206_TM=61.593 PRIMER_LEFT_206_GC_PERCENT=61.111 PRIMER_RIGHT_206_GC_PERCENT=55.000 PRIMER_LEFT_206_SELF_ANY=5.00 PRIMER_RIGHT_206_SELF_ANY=4.00 PRIMER_LEFT_206_SELF_END=1.00 PRIMER_RIGHT_206_SELF_END=1.00 PRIMER_LEFT_206_END_STABILITY=8.2000 PRIMER_RIGHT_206_END_STABILITY=6.6000 PRIMER_PAIR_206_COMPL_ANY=6.00 PRIMER_PAIR_206_COMPL_END=3.00 PRIMER_PAIR_206_PRODUCT_SIZE=75 PRIMER_PAIR_207_PENALTY=4.032768 PRIMER_LEFT_207_PENALTY=1.673968 PRIMER_RIGHT_207_PENALTY=2.358800 PRIMER_LEFT_207_SEQUENCE=TAAGTGATGCCCTTCTCGTTC PRIMER_RIGHT_207_SEQUENCE=CCAGTGAAGATGAGTTTCGCA PRIMER_LEFT_207=567,21 PRIMER_RIGHT_207=649,21 PRIMER_LEFT_207_TM=59.326 PRIMER_RIGHT_207_TM=61.359 PRIMER_LEFT_207_GC_PERCENT=47.619 PRIMER_RIGHT_207_GC_PERCENT=47.619 PRIMER_LEFT_207_SELF_ANY=4.00 PRIMER_RIGHT_207_SELF_ANY=3.00 PRIMER_LEFT_207_SELF_END=0.00 PRIMER_RIGHT_207_SELF_END=2.00 PRIMER_LEFT_207_END_STABILITY=8.4000 PRIMER_RIGHT_207_END_STABILITY=10.2000 PRIMER_PAIR_207_COMPL_ANY=4.00 PRIMER_PAIR_207_COMPL_END=1.00 PRIMER_PAIR_207_PRODUCT_SIZE=83 PRIMER_PAIR_208_PENALTY=4.033853 PRIMER_LEFT_208_PENALTY=1.604487 PRIMER_RIGHT_208_PENALTY=2.429366 PRIMER_LEFT_208_SEQUENCE=GAATGTCCAGAATGGGAAGTG PRIMER_RIGHT_208_SEQUENCE=GCAGGATGCCTTCCTTGGTA PRIMER_LEFT_208=334,21 PRIMER_RIGHT_208=410,20 PRIMER_LEFT_208_TM=59.396 PRIMER_RIGHT_208_TM=62.429 PRIMER_LEFT_208_GC_PERCENT=47.619 PRIMER_RIGHT_208_GC_PERCENT=55.000 PRIMER_LEFT_208_SELF_ANY=3.00 PRIMER_RIGHT_208_SELF_ANY=7.00 PRIMER_LEFT_208_SELF_END=0.00 PRIMER_RIGHT_208_SELF_END=2.00 PRIMER_LEFT_208_END_STABILITY=6.7000 PRIMER_RIGHT_208_END_STABILITY=7.2000 PRIMER_PAIR_208_COMPL_ANY=5.00 PRIMER_PAIR_208_COMPL_END=0.00 PRIMER_PAIR_208_PRODUCT_SIZE=77 PRIMER_PAIR_209_PENALTY=4.037527 PRIMER_LEFT_209_PENALTY=2.492700 PRIMER_RIGHT_209_PENALTY=1.544828 PRIMER_LEFT_209_SEQUENCE=AACCAGTGACCATCCAGAACT PRIMER_RIGHT_209_SEQUENCE=TCACCAACTAAGCAGCGGT PRIMER_LEFT_209=468,21 PRIMER_RIGHT_209=561,19 PRIMER_LEFT_209_TM=58.507 PRIMER_RIGHT_209_TM=59.455 PRIMER_LEFT_209_GC_PERCENT=47.619 PRIMER_RIGHT_209_GC_PERCENT=52.632 PRIMER_LEFT_209_SELF_ANY=3.00 PRIMER_RIGHT_209_SELF_ANY=3.00 PRIMER_LEFT_209_SELF_END=2.00 PRIMER_RIGHT_209_SELF_END=2.00 PRIMER_LEFT_209_END_STABILITY=6.4000 PRIMER_RIGHT_209_END_STABILITY=11.1000 PRIMER_PAIR_209_COMPL_ANY=4.00 PRIMER_PAIR_209_COMPL_END=1.00 PRIMER_PAIR_209_PRODUCT_SIZE=94 PRIMER_PAIR_210_PENALTY=4.039921 PRIMER_LEFT_210_PENALTY=2.290278 PRIMER_RIGHT_210_PENALTY=1.749643 PRIMER_LEFT_210_SEQUENCE=AGAAGCCAACCAACCAGTG PRIMER_RIGHT_210_SEQUENCE=AAGCAGCGGTAGGGAATCA PRIMER_LEFT_210=457,19 PRIMER_RIGHT_210=552,19 PRIMER_LEFT_210_TM=58.710 PRIMER_RIGHT_210_TM=60.750 PRIMER_LEFT_210_GC_PERCENT=52.632 PRIMER_RIGHT_210_GC_PERCENT=52.632 PRIMER_LEFT_210_SELF_ANY=3.00 PRIMER_RIGHT_210_SELF_ANY=3.00 PRIMER_LEFT_210_SELF_END=3.00 PRIMER_RIGHT_210_SELF_END=1.00 PRIMER_LEFT_210_END_STABILITY=6.7000 PRIMER_RIGHT_210_END_STABILITY=6.9000 PRIMER_PAIR_210_COMPL_ANY=4.00 PRIMER_PAIR_210_COMPL_END=2.00 PRIMER_PAIR_210_PRODUCT_SIZE=96 PRIMER_PAIR_211_PENALTY=4.052795 PRIMER_LEFT_211_PENALTY=2.066321 PRIMER_RIGHT_211_PENALTY=1.986473 PRIMER_LEFT_211_SEQUENCE=TCGTTCCTGACAAGTGCAA PRIMER_RIGHT_211_SEQUENCE=TGCATGTCTCTTTGGCGAC PRIMER_LEFT_211=582,19 PRIMER_RIGHT_211=674,19 PRIMER_LEFT_211_TM=58.934 PRIMER_RIGHT_211_TM=60.986 PRIMER_LEFT_211_GC_PERCENT=47.368 PRIMER_RIGHT_211_GC_PERCENT=52.632 PRIMER_LEFT_211_SELF_ANY=4.00 PRIMER_RIGHT_211_SELF_ANY=4.00 PRIMER_LEFT_211_SELF_END=2.00 PRIMER_RIGHT_211_SELF_END=2.00 PRIMER_LEFT_211_END_STABILITY=8.8000 PRIMER_RIGHT_211_END_STABILITY=9.6000 PRIMER_PAIR_211_COMPL_ANY=4.00 PRIMER_PAIR_211_COMPL_END=2.00 PRIMER_PAIR_211_PRODUCT_SIZE=93 PRIMER_PAIR_212_PENALTY=4.064698 PRIMER_LEFT_212_PENALTY=2.037339 PRIMER_RIGHT_212_PENALTY=2.027359 PRIMER_LEFT_212_SEQUENCE=TGGTGAGTTTGTAAGTGATGCC PRIMER_RIGHT_212_SEQUENCE=TGAGTTTCGCAAACATCCATC PRIMER_LEFT_212=556,22 PRIMER_RIGHT_212=639,21 PRIMER_LEFT_212_TM=60.037 PRIMER_RIGHT_212_TM=61.027 PRIMER_LEFT_212_GC_PERCENT=45.455 PRIMER_RIGHT_212_GC_PERCENT=42.857 PRIMER_LEFT_212_SELF_ANY=2.00 PRIMER_RIGHT_212_SELF_ANY=6.00 PRIMER_LEFT_212_SELF_END=1.00 PRIMER_RIGHT_212_SELF_END=0.00 PRIMER_LEFT_212_END_STABILITY=9.6000 PRIMER_RIGHT_212_END_STABILITY=8.1000 PRIMER_PAIR_212_COMPL_ANY=5.00 PRIMER_PAIR_212_COMPL_END=2.00 PRIMER_PAIR_212_PRODUCT_SIZE=84 PRIMER_PAIR_213_PENALTY=4.092033 PRIMER_LEFT_213_PENALTY=1.899540 PRIMER_RIGHT_213_PENALTY=2.192493 PRIMER_LEFT_213_SEQUENCE=TCTTCACTGGCACACCGTC PRIMER_RIGHT_213_SEQUENCE=CTTGTCAATTCCGCAGGG PRIMER_LEFT_213=640,19 PRIMER_RIGHT_213=733,18 PRIMER_LEFT_213_TM=60.900 PRIMER_RIGHT_213_TM=60.192 PRIMER_LEFT_213_GC_PERCENT=57.895 PRIMER_RIGHT_213_GC_PERCENT=55.556 PRIMER_LEFT_213_SELF_ANY=3.00 PRIMER_RIGHT_213_SELF_ANY=5.00 PRIMER_LEFT_213_SELF_END=1.00 PRIMER_RIGHT_213_SELF_END=1.00 PRIMER_LEFT_213_END_STABILITY=9.6000 PRIMER_RIGHT_213_END_STABILITY=9.7000 PRIMER_PAIR_213_COMPL_ANY=3.00 PRIMER_PAIR_213_COMPL_END=1.00 PRIMER_PAIR_213_PRODUCT_SIZE=94 PRIMER_PAIR_214_PENALTY=4.092753 PRIMER_LEFT_214_PENALTY=2.130358 PRIMER_RIGHT_214_PENALTY=1.962396 PRIMER_LEFT_214_SEQUENCE=ATTGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_214_SEQUENCE=GCACCAGTTCTGGATGGTC PRIMER_LEFT_214=414,22 PRIMER_RIGHT_214=493,19 PRIMER_LEFT_214_TM=60.130 PRIMER_RIGHT_214_TM=59.038 PRIMER_LEFT_214_GC_PERCENT=45.455 PRIMER_RIGHT_214_GC_PERCENT=57.895 PRIMER_LEFT_214_SELF_ANY=5.00 PRIMER_RIGHT_214_SELF_ANY=6.00 PRIMER_LEFT_214_SELF_END=1.00 PRIMER_RIGHT_214_SELF_END=3.00 PRIMER_LEFT_214_END_STABILITY=8.2000 PRIMER_RIGHT_214_END_STABILITY=7.9000 PRIMER_PAIR_214_COMPL_ANY=4.00 PRIMER_PAIR_214_COMPL_END=3.00 PRIMER_PAIR_214_PRODUCT_SIZE=80 PRIMER_PAIR_215_PENALTY=4.098568 PRIMER_LEFT_215_PENALTY=2.174817 PRIMER_RIGHT_215_PENALTY=1.923751 PRIMER_LEFT_215_SEQUENCE=ACCAAAACCTGCATTGATACC PRIMER_RIGHT_215_SEQUENCE=TTGGTTGGCTTCTACCACAT PRIMER_LEFT_215=374,21 PRIMER_RIGHT_215=469,20 PRIMER_LEFT_215_TM=58.825 PRIMER_RIGHT_215_TM=58.076 PRIMER_LEFT_215_GC_PERCENT=42.857 PRIMER_RIGHT_215_GC_PERCENT=45.000 PRIMER_LEFT_215_SELF_ANY=4.00 PRIMER_RIGHT_215_SELF_ANY=4.00 PRIMER_LEFT_215_SELF_END=2.00 PRIMER_RIGHT_215_SELF_END=2.00 PRIMER_LEFT_215_END_STABILITY=6.8000 PRIMER_RIGHT_215_END_STABILITY=6.6000 PRIMER_PAIR_215_COMPL_ANY=5.00 PRIMER_PAIR_215_COMPL_END=1.00 PRIMER_PAIR_215_PRODUCT_SIZE=96 PRIMER_PAIR_216_PENALTY=4.102484 PRIMER_LEFT_216_PENALTY=2.486079 PRIMER_RIGHT_216_PENALTY=1.616405 PRIMER_LEFT_216_SEQUENCE=CAAGCAGTGCAAGACCCA PRIMER_RIGHT_216_SEQUENCE=GGAACGAGAAGGGCATCAC PRIMER_LEFT_216=505,18 PRIMER_RIGHT_216=588,19 PRIMER_LEFT_216_TM=59.514 PRIMER_RIGHT_216_TM=60.616 PRIMER_LEFT_216_GC_PERCENT=55.556 PRIMER_RIGHT_216_GC_PERCENT=57.895 PRIMER_LEFT_216_SELF_ANY=5.00 PRIMER_RIGHT_216_SELF_ANY=2.00 PRIMER_LEFT_216_SELF_END=0.00 PRIMER_RIGHT_216_SELF_END=2.00 PRIMER_LEFT_216_END_STABILITY=9.4000 PRIMER_RIGHT_216_END_STABILITY=6.3000 PRIMER_PAIR_216_COMPL_ANY=3.00 PRIMER_PAIR_216_COMPL_END=2.00 PRIMER_PAIR_216_PRODUCT_SIZE=84 PRIMER_PAIR_217_PENALTY=4.109720 PRIMER_LEFT_217_PENALTY=2.024215 PRIMER_RIGHT_217_PENALTY=2.085505 PRIMER_LEFT_217_SEQUENCE=CCCTACCGCTGCTTAGTTGG PRIMER_RIGHT_217_SEQUENCE=GTTTCGCAAACATCCATCC PRIMER_LEFT_217=539,20 PRIMER_RIGHT_217=636,19 PRIMER_LEFT_217_TM=62.024 PRIMER_RIGHT_217_TM=58.914 PRIMER_LEFT_217_GC_PERCENT=60.000 PRIMER_RIGHT_217_GC_PERCENT=47.368 PRIMER_LEFT_217_SELF_ANY=3.00 PRIMER_RIGHT_217_SELF_ANY=6.00 PRIMER_LEFT_217_SELF_END=0.00 PRIMER_RIGHT_217_SELF_END=0.00 PRIMER_LEFT_217_END_STABILITY=8.2000 PRIMER_RIGHT_217_END_STABILITY=8.1000 PRIMER_PAIR_217_COMPL_ANY=3.00 PRIMER_PAIR_217_COMPL_END=2.00 PRIMER_PAIR_217_PRODUCT_SIZE=98 PRIMER_PAIR_218_PENALTY=4.111462 PRIMER_LEFT_218_PENALTY=1.752662 PRIMER_RIGHT_218_PENALTY=2.358800 PRIMER_LEFT_218_SEQUENCE=GATGCCCTTCTCGTTCCTG PRIMER_RIGHT_218_SEQUENCE=TGCCAGTGAAGATGAGTTTCG PRIMER_LEFT_218=572,19 PRIMER_RIGHT_218=651,21 PRIMER_LEFT_218_TM=60.753 PRIMER_RIGHT_218_TM=61.359 PRIMER_LEFT_218_GC_PERCENT=57.895 PRIMER_RIGHT_218_GC_PERCENT=47.619 PRIMER_LEFT_218_SELF_ANY=2.00 PRIMER_RIGHT_218_SELF_ANY=3.00 PRIMER_LEFT_218_SELF_END=1.00 PRIMER_RIGHT_218_SELF_END=2.00 PRIMER_LEFT_218_END_STABILITY=8.2000 PRIMER_RIGHT_218_END_STABILITY=9.0000 PRIMER_PAIR_218_COMPL_ANY=4.00 PRIMER_PAIR_218_COMPL_END=0.00 PRIMER_PAIR_218_PRODUCT_SIZE=80 PRIMER_PAIR_219_PENALTY=4.114989 PRIMER_LEFT_219_PENALTY=2.186531 PRIMER_RIGHT_219_PENALTY=1.928458 PRIMER_LEFT_219_SEQUENCE=AGGGACCAAAACCTGCATTG PRIMER_RIGHT_219_SEQUENCE=TGGTTGGCTTCTACCACATTG PRIMER_LEFT_219=370,20 PRIMER_RIGHT_219=468,21 PRIMER_LEFT_219_TM=62.187 PRIMER_RIGHT_219_TM=60.928 PRIMER_LEFT_219_GC_PERCENT=50.000 PRIMER_RIGHT_219_GC_PERCENT=47.619 PRIMER_LEFT_219_SELF_ANY=4.00 PRIMER_RIGHT_219_SELF_ANY=4.00 PRIMER_LEFT_219_SELF_END=3.00 PRIMER_RIGHT_219_SELF_END=3.00 PRIMER_LEFT_219_END_STABILITY=7.2000 PRIMER_RIGHT_219_END_STABILITY=7.2000 PRIMER_PAIR_219_COMPL_ANY=4.00 PRIMER_PAIR_219_COMPL_END=3.00 PRIMER_PAIR_219_PRODUCT_SIZE=99 PRIMER_PAIR_220_PENALTY=4.118223 PRIMER_LEFT_220_PENALTY=2.561455 PRIMER_RIGHT_220_PENALTY=1.556768 PRIMER_LEFT_220_SEQUENCE=ATGCACATGAATGTCCAGAA PRIMER_RIGHT_220_SEQUENCE=TTCCTTGGTATCAATGCAGGT PRIMER_LEFT_220=326,20 PRIMER_RIGHT_220=400,21 PRIMER_LEFT_220_TM=57.439 PRIMER_RIGHT_220_TM=59.443 PRIMER_LEFT_220_GC_PERCENT=40.000 PRIMER_RIGHT_220_GC_PERCENT=42.857 PRIMER_LEFT_220_SELF_ANY=8.00 PRIMER_RIGHT_220_SELF_ANY=6.00 PRIMER_LEFT_220_SELF_END=1.00 PRIMER_RIGHT_220_SELF_END=0.00 PRIMER_LEFT_220_END_STABILITY=7.0000 PRIMER_RIGHT_220_END_STABILITY=7.9000 PRIMER_PAIR_220_COMPL_ANY=4.00 PRIMER_PAIR_220_COMPL_END=2.00 PRIMER_PAIR_220_PRODUCT_SIZE=75 PRIMER_PAIR_221_PENALTY=4.120357 PRIMER_LEFT_221_PENALTY=2.074556 PRIMER_RIGHT_221_PENALTY=2.045801 PRIMER_LEFT_221_SEQUENCE=ATCACCAATGTGGTAGAAGCC PRIMER_RIGHT_221_SEQUENCE=GGGATGGGTCTTGCACTG PRIMER_LEFT_221=443,21 PRIMER_RIGHT_221=526,18 PRIMER_LEFT_221_TM=58.925 PRIMER_RIGHT_221_TM=60.046 PRIMER_LEFT_221_GC_PERCENT=47.619 PRIMER_RIGHT_221_GC_PERCENT=61.111 PRIMER_LEFT_221_SELF_ANY=6.00 PRIMER_RIGHT_221_SELF_ANY=4.00 PRIMER_LEFT_221_SELF_END=1.00 PRIMER_RIGHT_221_SELF_END=3.00 PRIMER_LEFT_221_END_STABILITY=9.7000 PRIMER_RIGHT_221_END_STABILITY=6.7000 PRIMER_PAIR_221_COMPL_ANY=4.00 PRIMER_PAIR_221_COMPL_END=1.00 PRIMER_PAIR_221_PRODUCT_SIZE=84 PRIMER_PAIR_222_PENALTY=4.130443 PRIMER_LEFT_222_PENALTY=2.402111 PRIMER_RIGHT_222_PENALTY=1.728332 PRIMER_LEFT_222_SEQUENCE=CACCGTCGCCAAAGAGAC PRIMER_RIGHT_222_SEQUENCE=TACCCCTCGGAACTTGTCAAT PRIMER_LEFT_222=652,18 PRIMER_RIGHT_222=745,21 PRIMER_LEFT_222_TM=60.402 PRIMER_RIGHT_222_TM=60.728 PRIMER_LEFT_222_GC_PERCENT=61.111 PRIMER_RIGHT_222_GC_PERCENT=47.619 PRIMER_LEFT_222_SELF_ANY=3.00 PRIMER_RIGHT_222_SELF_ANY=5.00 PRIMER_LEFT_222_SELF_END=2.00 PRIMER_RIGHT_222_SELF_END=3.00 PRIMER_LEFT_222_END_STABILITY=6.1000 PRIMER_RIGHT_222_END_STABILITY=6.9000 PRIMER_PAIR_222_COMPL_ANY=3.00 PRIMER_PAIR_222_COMPL_END=2.00 PRIMER_PAIR_222_PRODUCT_SIZE=94 PRIMER_PAIR_223_PENALTY=4.147260 PRIMER_LEFT_223_PENALTY=2.402111 PRIMER_RIGHT_223_PENALTY=1.745148 PRIMER_LEFT_223_SEQUENCE=ACACCGTCGCCAAAGAGA PRIMER_RIGHT_223_SEQUENCE=CTCTACCCCTCGGAACTTGT PRIMER_LEFT_223=651,18 PRIMER_RIGHT_223=748,20 PRIMER_LEFT_223_TM=60.402 PRIMER_RIGHT_223_TM=58.255 PRIMER_LEFT_223_GC_PERCENT=55.556 PRIMER_RIGHT_223_GC_PERCENT=55.000 PRIMER_LEFT_223_SELF_ANY=3.00 PRIMER_RIGHT_223_SELF_ANY=3.00 PRIMER_LEFT_223_SELF_END=0.00 PRIMER_RIGHT_223_SELF_END=2.00 PRIMER_LEFT_223_END_STABILITY=6.4000 PRIMER_RIGHT_223_END_STABILITY=6.7000 PRIMER_PAIR_223_COMPL_ANY=4.00 PRIMER_PAIR_223_COMPL_END=1.00 PRIMER_PAIR_223_PRODUCT_SIZE=98 PRIMER_PAIR_224_PENALTY=4.154774 PRIMER_LEFT_224_PENALTY=1.752662 PRIMER_RIGHT_224_PENALTY=2.402111 PRIMER_LEFT_224_SEQUENCE=TGATGCCCTTCTCGTTCCT PRIMER_RIGHT_224_SEQUENCE=TCTCTTTGGCGACGGTGT PRIMER_LEFT_224=571,19 PRIMER_RIGHT_224=668,18 PRIMER_LEFT_224_TM=60.753 PRIMER_RIGHT_224_TM=60.402 PRIMER_LEFT_224_GC_PERCENT=52.632 PRIMER_RIGHT_224_GC_PERCENT=55.556 PRIMER_LEFT_224_SELF_ANY=2.00 PRIMER_RIGHT_224_SELF_ANY=3.00 PRIMER_LEFT_224_SELF_END=0.00 PRIMER_RIGHT_224_SELF_END=1.00 PRIMER_LEFT_224_END_STABILITY=8.2000 PRIMER_RIGHT_224_END_STABILITY=7.6000 PRIMER_PAIR_224_COMPL_ANY=3.00 PRIMER_PAIR_224_COMPL_END=0.00 PRIMER_PAIR_224_PRODUCT_SIZE=98 PRIMER_PAIR_225_PENALTY=4.157833 PRIMER_LEFT_225_PENALTY=1.651669 PRIMER_RIGHT_225_PENALTY=2.506164 PRIMER_LEFT_225_SEQUENCE=TCCCCACTTTGTGATTCCCT PRIMER_RIGHT_225_SEQUENCE=TGCACTTGTCAGGAACGAG PRIMER_LEFT_225=523,20 PRIMER_RIGHT_225=599,19 PRIMER_LEFT_225_TM=61.652 PRIMER_RIGHT_225_TM=58.494 PRIMER_LEFT_225_GC_PERCENT=50.000 PRIMER_RIGHT_225_GC_PERCENT=52.632 PRIMER_LEFT_225_SELF_ANY=3.00 PRIMER_RIGHT_225_SELF_ANY=4.00 PRIMER_LEFT_225_SELF_END=1.00 PRIMER_RIGHT_225_SELF_END=2.00 PRIMER_LEFT_225_END_STABILITY=9.4000 PRIMER_RIGHT_225_END_STABILITY=8.1000 PRIMER_PAIR_225_COMPL_ANY=4.00 PRIMER_PAIR_225_COMPL_END=2.00 PRIMER_PAIR_225_PRODUCT_SIZE=77 PRIMER_PAIR_226_PENALTY=4.205638 PRIMER_LEFT_226_PENALTY=2.282356 PRIMER_RIGHT_226_PENALTY=1.923282 PRIMER_LEFT_226_SEQUENCE=AATGTGGTAGAAGCCAACCAAC PRIMER_RIGHT_226_SEQUENCE=CGGTAGGGAATCACAAAGTG PRIMER_LEFT_226=449,22 PRIMER_RIGHT_226=546,20 PRIMER_LEFT_226_TM=60.282 PRIMER_RIGHT_226_TM=58.077 PRIMER_LEFT_226_GC_PERCENT=45.455 PRIMER_RIGHT_226_GC_PERCENT=50.000 PRIMER_LEFT_226_SELF_ANY=4.00 PRIMER_RIGHT_226_SELF_ANY=3.00 PRIMER_LEFT_226_SELF_END=2.00 PRIMER_RIGHT_226_SELF_END=3.00 PRIMER_LEFT_226_END_STABILITY=8.2000 PRIMER_RIGHT_226_END_STABILITY=6.7000 PRIMER_PAIR_226_COMPL_ANY=4.00 PRIMER_PAIR_226_COMPL_END=1.00 PRIMER_PAIR_226_PRODUCT_SIZE=98 PRIMER_PAIR_227_PENALTY=4.221294 PRIMER_LEFT_227_PENALTY=2.547327 PRIMER_RIGHT_227_PENALTY=1.673968 PRIMER_LEFT_227_SEQUENCE=AAGCAGTGCAAGACCCATCC PRIMER_RIGHT_227_SEQUENCE=GAACGAGAAGGGCATCACTTA PRIMER_LEFT_227=506,20 PRIMER_RIGHT_227=587,21 PRIMER_LEFT_227_TM=62.547 PRIMER_RIGHT_227_TM=59.326 PRIMER_LEFT_227_GC_PERCENT=55.000 PRIMER_RIGHT_227_GC_PERCENT=47.619 PRIMER_LEFT_227_SELF_ANY=5.00 PRIMER_RIGHT_227_SELF_ANY=4.00 PRIMER_LEFT_227_SELF_END=0.00 PRIMER_RIGHT_227_SELF_END=2.00 PRIMER_LEFT_227_END_STABILITY=8.1000 PRIMER_RIGHT_227_END_STABILITY=5.7000 PRIMER_PAIR_227_COMPL_ANY=5.00 PRIMER_PAIR_227_COMPL_END=2.00 PRIMER_PAIR_227_PRODUCT_SIZE=82 PRIMER_PAIR_228_PENALTY=4.221881 PRIMER_LEFT_228_PENALTY=2.617395 PRIMER_RIGHT_228_PENALTY=1.604487 PRIMER_LEFT_228_SEQUENCE=GTACCCACTGATGGTAATGCTG PRIMER_RIGHT_228_SEQUENCE=CACTTCCCATTCTGGACATTC PRIMER_LEFT_228=257,22 PRIMER_RIGHT_228=354,21 PRIMER_LEFT_228_TM=59.383 PRIMER_RIGHT_228_TM=59.396 PRIMER_LEFT_228_GC_PERCENT=50.000 PRIMER_RIGHT_228_GC_PERCENT=47.619 PRIMER_LEFT_228_SELF_ANY=5.00 PRIMER_RIGHT_228_SELF_ANY=3.00 PRIMER_LEFT_228_SELF_END=1.00 PRIMER_RIGHT_228_SELF_END=2.00 PRIMER_LEFT_228_END_STABILITY=8.5000 PRIMER_RIGHT_228_END_STABILITY=6.9000 PRIMER_PAIR_228_COMPL_ANY=5.00 PRIMER_PAIR_228_COMPL_END=1.00 PRIMER_PAIR_228_PRODUCT_SIZE=98 PRIMER_PAIR_229_PENALTY=4.232776 PRIMER_LEFT_229_PENALTY=2.994694 PRIMER_RIGHT_229_PENALTY=1.238082 PRIMER_LEFT_229_SEQUENCE=ATTGATACCAAGGAAGGCATC PRIMER_RIGHT_229_SEQUENCE=GATGGTCACTGGTTGGTTGG PRIMER_LEFT_229=386,21 PRIMER_RIGHT_229=481,20 PRIMER_LEFT_229_TM=58.005 PRIMER_RIGHT_229_TM=61.238 PRIMER_LEFT_229_GC_PERCENT=42.857 PRIMER_RIGHT_229_GC_PERCENT=55.000 PRIMER_LEFT_229_SELF_ANY=6.00 PRIMER_RIGHT_229_SELF_ANY=3.00 PRIMER_LEFT_229_SELF_END=3.00 PRIMER_RIGHT_229_SELF_END=0.00 PRIMER_LEFT_229_END_STABILITY=8.1000 PRIMER_RIGHT_229_END_STABILITY=8.2000 PRIMER_PAIR_229_COMPL_ANY=5.00 PRIMER_PAIR_229_COMPL_END=1.00 PRIMER_PAIR_229_PRODUCT_SIZE=96 PRIMER_PAIR_230_PENALTY=4.286251 PRIMER_LEFT_230_PENALTY=2.215444 PRIMER_RIGHT_230_PENALTY=2.070807 PRIMER_LEFT_230_SEQUENCE=CCCTTCTCGTTCCTGACAA PRIMER_RIGHT_230_SEQUENCE=TGCATGTCTCTTTGGCGA PRIMER_LEFT_230=576,19 PRIMER_RIGHT_230=674,18 PRIMER_LEFT_230_TM=58.785 PRIMER_RIGHT_230_TM=60.071 PRIMER_LEFT_230_GC_PERCENT=52.632 PRIMER_RIGHT_230_GC_PERCENT=50.000 PRIMER_LEFT_230_SELF_ANY=3.00 PRIMER_RIGHT_230_SELF_ANY=4.00 PRIMER_LEFT_230_SELF_END=1.00 PRIMER_RIGHT_230_SELF_END=2.00 PRIMER_LEFT_230_END_STABILITY=6.7000 PRIMER_RIGHT_230_END_STABILITY=11.4000 PRIMER_PAIR_230_COMPL_ANY=4.00 PRIMER_PAIR_230_COMPL_END=2.00 PRIMER_PAIR_230_PRODUCT_SIZE=99 PRIMER_PAIR_231_PENALTY=4.308743 PRIMER_LEFT_231_PENALTY=2.567306 PRIMER_RIGHT_231_PENALTY=1.741437 PRIMER_LEFT_231_SEQUENCE=GTTTGGCACTGCTCCTGC PRIMER_RIGHT_231_SEQUENCE=ACATGGCAATCTGGGGTTC PRIMER_LEFT_231=210,18 PRIMER_RIGHT_231=308,19 PRIMER_LEFT_231_TM=60.567 PRIMER_RIGHT_231_TM=60.741 PRIMER_LEFT_231_GC_PERCENT=61.111 PRIMER_RIGHT_231_GC_PERCENT=52.632 PRIMER_LEFT_231_SELF_ANY=5.00 PRIMER_RIGHT_231_SELF_ANY=4.00 PRIMER_LEFT_231_SELF_END=2.00 PRIMER_RIGHT_231_SELF_END=0.00 PRIMER_LEFT_231_END_STABILITY=9.7000 PRIMER_RIGHT_231_END_STABILITY=7.9000 PRIMER_PAIR_231_COMPL_ANY=4.00 PRIMER_PAIR_231_COMPL_END=0.00 PRIMER_PAIR_231_PRODUCT_SIZE=99 PRIMER_PAIR_232_PENALTY=4.314672 PRIMER_LEFT_232_PENALTY=1.709051 PRIMER_RIGHT_232_PENALTY=2.605621 PRIMER_LEFT_232_SEQUENCE=TCCCCACTTTGTGATTCCC PRIMER_RIGHT_232_SEQUENCE=TTTGCACTTGTCAGGAACG PRIMER_LEFT_232=523,19 PRIMER_RIGHT_232=601,19 PRIMER_LEFT_232_TM=60.709 PRIMER_RIGHT_232_TM=58.394 PRIMER_LEFT_232_GC_PERCENT=52.632 PRIMER_RIGHT_232_GC_PERCENT=47.368 PRIMER_LEFT_232_SELF_ANY=3.00 PRIMER_RIGHT_232_SELF_ANY=4.00 PRIMER_LEFT_232_SELF_END=3.00 PRIMER_RIGHT_232_SELF_END=2.00 PRIMER_LEFT_232_END_STABILITY=9.7000 PRIMER_RIGHT_232_END_STABILITY=8.4000 PRIMER_PAIR_232_COMPL_ANY=4.00 PRIMER_PAIR_232_COMPL_END=1.00 PRIMER_PAIR_232_PRODUCT_SIZE=79 PRIMER_PAIR_233_PENALTY=4.314902 PRIMER_LEFT_233_PENALTY=2.296862 PRIMER_RIGHT_233_PENALTY=2.018039 PRIMER_LEFT_233_SEQUENCE=AAGCCAACCAACCAGTGACC PRIMER_RIGHT_233_SEQUENCE=ACTAAGCAGCGGTAGGGAAT PRIMER_LEFT_233=459,20 PRIMER_RIGHT_233=555,20 PRIMER_LEFT_233_TM=62.297 PRIMER_RIGHT_233_TM=57.982 PRIMER_LEFT_233_GC_PERCENT=55.000 PRIMER_RIGHT_233_GC_PERCENT=50.000 PRIMER_LEFT_233_SELF_ANY=3.00 PRIMER_RIGHT_233_SELF_ANY=3.00 PRIMER_LEFT_233_SELF_END=1.00 PRIMER_RIGHT_233_SELF_END=2.00 PRIMER_LEFT_233_END_STABILITY=7.9000 PRIMER_RIGHT_233_END_STABILITY=8.1000 PRIMER_PAIR_233_COMPL_ANY=4.00 PRIMER_PAIR_233_COMPL_END=0.00 PRIMER_PAIR_233_PRODUCT_SIZE=97 PRIMER_PAIR_234_PENALTY=4.315389 PRIMER_LEFT_234_PENALTY=2.413667 PRIMER_RIGHT_234_PENALTY=1.901722 PRIMER_LEFT_234_SEQUENCE=CGCTGGAGGTACCCACTGAT PRIMER_RIGHT_234_SEQUENCE=TCATGTGCATGTTCAGTCTGC PRIMER_LEFT_234=249,20 PRIMER_RIGHT_234=335,21 PRIMER_LEFT_234_TM=62.414 PRIMER_RIGHT_234_TM=60.902 PRIMER_LEFT_234_GC_PERCENT=60.000 PRIMER_RIGHT_234_GC_PERCENT=47.619 PRIMER_LEFT_234_SELF_ANY=6.00 PRIMER_RIGHT_234_SELF_ANY=6.00 PRIMER_LEFT_234_SELF_END=2.00 PRIMER_RIGHT_234_SELF_END=3.00 PRIMER_LEFT_234_END_STABILITY=6.6000 PRIMER_RIGHT_234_END_STABILITY=8.2000 PRIMER_PAIR_234_COMPL_ANY=5.00 PRIMER_PAIR_234_COMPL_END=2.00 PRIMER_PAIR_234_PRODUCT_SIZE=87 PRIMER_PAIR_235_PENALTY=4.318474 PRIMER_LEFT_235_PENALTY=2.942733 PRIMER_RIGHT_235_PENALTY=1.375741 PRIMER_LEFT_235_SEQUENCE=CGCCAAAGAGACATGCAG PRIMER_RIGHT_235_SEQUENCE=CAAACTCTACCCCTCGGAACT PRIMER_LEFT_235=658,18 PRIMER_RIGHT_235=752,21 PRIMER_LEFT_235_TM=59.057 PRIMER_RIGHT_235_TM=59.624 PRIMER_LEFT_235_GC_PERCENT=55.556 PRIMER_RIGHT_235_GC_PERCENT=52.381 PRIMER_LEFT_235_SELF_ANY=4.00 PRIMER_RIGHT_235_SELF_ANY=3.00 PRIMER_LEFT_235_SELF_END=2.00 PRIMER_RIGHT_235_SELF_END=1.00 PRIMER_LEFT_235_END_STABILITY=8.5000 PRIMER_RIGHT_235_END_STABILITY=6.4000 PRIMER_PAIR_235_COMPL_ANY=4.00 PRIMER_PAIR_235_COMPL_END=2.00 PRIMER_PAIR_235_PRODUCT_SIZE=95 PRIMER_PAIR_236_PENALTY=4.320583 PRIMER_LEFT_236_PENALTY=2.292791 PRIMER_RIGHT_236_PENALTY=2.027792 PRIMER_LEFT_236_SEQUENCE=GGGACCAAAACCTGCATTG PRIMER_RIGHT_236_SEQUENCE=GTTGGTTGGCTTCTACCACAT PRIMER_LEFT_236=371,19 PRIMER_RIGHT_236=470,21 PRIMER_LEFT_236_TM=61.293 PRIMER_RIGHT_236_TM=58.972 PRIMER_LEFT_236_GC_PERCENT=52.632 PRIMER_RIGHT_236_GC_PERCENT=47.619 PRIMER_LEFT_236_SELF_ANY=4.00 PRIMER_RIGHT_236_SELF_ANY=4.00 PRIMER_LEFT_236_SELF_END=3.00 PRIMER_RIGHT_236_SELF_END=2.00 PRIMER_LEFT_236_END_STABILITY=7.2000 PRIMER_RIGHT_236_END_STABILITY=6.6000 PRIMER_PAIR_236_COMPL_ANY=5.00 PRIMER_PAIR_236_COMPL_END=2.00 PRIMER_PAIR_236_PRODUCT_SIZE=100 PRIMER_PAIR_237_PENALTY=4.340715 PRIMER_LEFT_237_PENALTY=1.190908 PRIMER_RIGHT_237_PENALTY=3.149807 PRIMER_LEFT_237_SEQUENCE=ACCAGGAGAGGATGGATGTTT PRIMER_RIGHT_237_SEQUENCE=TGCCGTAGTCATGCAAGTT PRIMER_LEFT_237=609,21 PRIMER_RIGHT_237=707,19 PRIMER_LEFT_237_TM=59.809 PRIMER_RIGHT_237_TM=57.850 PRIMER_LEFT_237_GC_PERCENT=47.619 PRIMER_RIGHT_237_GC_PERCENT=47.368 PRIMER_LEFT_237_SELF_ANY=3.00 PRIMER_RIGHT_237_SELF_ANY=4.00 PRIMER_LEFT_237_SELF_END=0.00 PRIMER_RIGHT_237_SELF_END=3.00 PRIMER_LEFT_237_END_STABILITY=7.0000 PRIMER_RIGHT_237_END_STABILITY=6.7000 PRIMER_PAIR_237_COMPL_ANY=4.00 PRIMER_PAIR_237_COMPL_END=1.00 PRIMER_PAIR_237_PRODUCT_SIZE=99 PRIMER_PAIR_238_PENALTY=4.348500 PRIMER_LEFT_238_PENALTY=0.949861 PRIMER_RIGHT_238_PENALTY=3.398638 PRIMER_LEFT_238_SEQUENCE=ATCCATCAGGGACCAAAACC PRIMER_RIGHT_238_SEQUENCE=TCTGCAGTTCAGGGTAGACTTC PRIMER_LEFT_238=363,20 PRIMER_RIGHT_238=443,22 PRIMER_LEFT_238_TM=60.950 PRIMER_RIGHT_238_TM=58.601 PRIMER_LEFT_238_GC_PERCENT=50.000 PRIMER_RIGHT_238_GC_PERCENT=50.000 PRIMER_LEFT_238_SELF_ANY=5.00 PRIMER_RIGHT_238_SELF_ANY=6.00 PRIMER_LEFT_238_SELF_END=0.00 PRIMER_RIGHT_238_SELF_END=3.00 PRIMER_LEFT_238_END_STABILITY=8.2000 PRIMER_RIGHT_238_END_STABILITY=6.4000 PRIMER_PAIR_238_COMPL_ANY=3.00 PRIMER_PAIR_238_COMPL_END=0.00 PRIMER_PAIR_238_PRODUCT_SIZE=81 PRIMER_PAIR_239_PENALTY=4.377818 PRIMER_LEFT_239_PENALTY=2.253482 PRIMER_RIGHT_239_PENALTY=2.124336 PRIMER_LEFT_239_SEQUENCE=TGCAAATTCTTACACCAGGAGA PRIMER_RIGHT_239_SEQUENCE=CTGCATGTCTCTTTGGCGA PRIMER_LEFT_239=596,22 PRIMER_RIGHT_239=675,19 PRIMER_LEFT_239_TM=59.747 PRIMER_RIGHT_239_TM=61.124 PRIMER_LEFT_239_GC_PERCENT=40.909 PRIMER_RIGHT_239_GC_PERCENT=52.632 PRIMER_LEFT_239_SELF_ANY=4.00 PRIMER_RIGHT_239_SELF_ANY=4.00 PRIMER_LEFT_239_SELF_END=1.00 PRIMER_RIGHT_239_SELF_END=2.00 PRIMER_LEFT_239_END_STABILITY=7.9000 PRIMER_RIGHT_239_END_STABILITY=11.4000 PRIMER_PAIR_239_COMPL_ANY=4.00 PRIMER_PAIR_239_COMPL_END=3.00 PRIMER_PAIR_239_PRODUCT_SIZE=80 PRIMER_PAIR_240_PENALTY=4.400163 PRIMER_LEFT_240_PENALTY=3.162081 PRIMER_RIGHT_240_PENALTY=1.238082 PRIMER_LEFT_240_SEQUENCE=CCTGCAGTATTGCCAAGAAGTC PRIMER_RIGHT_240_SEQUENCE=GGATGGTCACTGGTTGGTTG PRIMER_LEFT_240=406,22 PRIMER_RIGHT_240=482,20 PRIMER_LEFT_240_TM=61.162 PRIMER_RIGHT_240_TM=61.238 PRIMER_LEFT_240_GC_PERCENT=50.000 PRIMER_RIGHT_240_GC_PERCENT=55.000 PRIMER_LEFT_240_SELF_ANY=6.00 PRIMER_RIGHT_240_SELF_ANY=3.00 PRIMER_LEFT_240_SELF_END=2.00 PRIMER_RIGHT_240_SELF_END=0.00 PRIMER_LEFT_240_END_STABILITY=6.4000 PRIMER_RIGHT_240_END_STABILITY=8.2000 PRIMER_PAIR_240_COMPL_ANY=5.00 PRIMER_PAIR_240_COMPL_END=1.00 PRIMER_PAIR_240_PRODUCT_SIZE=77 PRIMER_PAIR_241_PENALTY=4.400163 PRIMER_LEFT_241_PENALTY=3.162081 PRIMER_RIGHT_241_PENALTY=1.238082 PRIMER_LEFT_241_SEQUENCE=TCCTGCAGTATTGCCAAGAAGT PRIMER_RIGHT_241_SEQUENCE=TGGATGGTCACTGGTTGGTT PRIMER_LEFT_241=405,22 PRIMER_RIGHT_241=483,20 PRIMER_LEFT_241_TM=61.162 PRIMER_RIGHT_241_TM=61.238 PRIMER_LEFT_241_GC_PERCENT=45.455 PRIMER_RIGHT_241_GC_PERCENT=50.000 PRIMER_LEFT_241_SELF_ANY=6.00 PRIMER_RIGHT_241_SELF_ANY=3.00 PRIMER_LEFT_241_SELF_END=1.00 PRIMER_RIGHT_241_SELF_END=0.00 PRIMER_LEFT_241_END_STABILITY=6.4000 PRIMER_RIGHT_241_END_STABILITY=8.2000 PRIMER_PAIR_241_COMPL_ANY=5.00 PRIMER_PAIR_241_COMPL_END=3.00 PRIMER_PAIR_241_PRODUCT_SIZE=79 PRIMER_PAIR_242_PENALTY=4.407754 PRIMER_LEFT_242_PENALTY=2.192310 PRIMER_RIGHT_242_PENALTY=2.215444 PRIMER_LEFT_242_SEQUENCE=ACCCATCCCCACTTTGTGA PRIMER_RIGHT_242_SEQUENCE=TTGTCAGGAACGAGAAGGG PRIMER_LEFT_242=518,19 PRIMER_RIGHT_242=594,19 PRIMER_LEFT_242_TM=61.192 PRIMER_RIGHT_242_TM=58.785 PRIMER_LEFT_242_GC_PERCENT=52.632 PRIMER_RIGHT_242_GC_PERCENT=52.632 PRIMER_LEFT_242_SELF_ANY=3.00 PRIMER_RIGHT_242_SELF_ANY=3.00 PRIMER_LEFT_242_SELF_END=2.00 PRIMER_RIGHT_242_SELF_END=0.00 PRIMER_LEFT_242_END_STABILITY=6.7000 PRIMER_RIGHT_242_END_STABILITY=9.7000 PRIMER_PAIR_242_COMPL_ANY=4.00 PRIMER_PAIR_242_COMPL_END=2.00 PRIMER_PAIR_242_PRODUCT_SIZE=77 PRIMER_PAIR_243_PENALTY=4.410865 PRIMER_LEFT_243_PENALTY=1.923282 PRIMER_RIGHT_243_PENALTY=2.487583 PRIMER_LEFT_243_SEQUENCE=CACTTTGTGATTCCCTACCG PRIMER_RIGHT_243_SEQUENCE=CCATCCTCTCCTGGTGTAAGAA PRIMER_LEFT_243=527,20 PRIMER_RIGHT_243=623,22 PRIMER_LEFT_243_TM=58.077 PRIMER_RIGHT_243_TM=60.488 PRIMER_LEFT_243_GC_PERCENT=50.000 PRIMER_RIGHT_243_GC_PERCENT=50.000 PRIMER_LEFT_243_SELF_ANY=3.00 PRIMER_RIGHT_243_SELF_ANY=3.00 PRIMER_LEFT_243_SELF_END=2.00 PRIMER_RIGHT_243_SELF_END=1.00 PRIMER_LEFT_243_END_STABILITY=8.9000 PRIMER_RIGHT_243_END_STABILITY=7.0000 PRIMER_PAIR_243_COMPL_ANY=3.00 PRIMER_PAIR_243_COMPL_END=1.00 PRIMER_PAIR_243_PRODUCT_SIZE=97 PRIMER_PAIR_244_PENALTY=4.424294 PRIMER_LEFT_244_PENALTY=3.348683 PRIMER_RIGHT_244_PENALTY=1.075611 PRIMER_LEFT_244_SEQUENCE=CACTGATGGTAATGCTGGCCT PRIMER_RIGHT_244_SEQUENCE=AATCCCACTTCCCATTCTGG PRIMER_LEFT_244=262,21 PRIMER_RIGHT_244=359,20 PRIMER_LEFT_244_TM=62.349 PRIMER_RIGHT_244_TM=61.076 PRIMER_LEFT_244_GC_PERCENT=52.381 PRIMER_RIGHT_244_GC_PERCENT=50.000 PRIMER_LEFT_244_SELF_ANY=4.00 PRIMER_RIGHT_244_SELF_ANY=3.00 PRIMER_LEFT_244_SELF_END=2.00 PRIMER_RIGHT_244_SELF_END=3.00 PRIMER_LEFT_244_END_STABILITY=10.9000 PRIMER_RIGHT_244_END_STABILITY=8.2000 PRIMER_PAIR_244_COMPL_ANY=5.00 PRIMER_PAIR_244_COMPL_END=2.00 PRIMER_PAIR_244_PRODUCT_SIZE=98 PRIMER_PAIR_245_PENALTY=4.431970 PRIMER_LEFT_245_PENALTY=2.619082 PRIMER_RIGHT_245_PENALTY=1.812888 PRIMER_LEFT_245_SEQUENCE=AACCAGTGACCATCCAGAAC PRIMER_RIGHT_245_SEQUENCE=AACTCACCAACTAAGCAGCG PRIMER_LEFT_245=468,20 PRIMER_RIGHT_245=564,20 PRIMER_LEFT_245_TM=57.381 PRIMER_RIGHT_245_TM=58.187 PRIMER_LEFT_245_GC_PERCENT=50.000 PRIMER_RIGHT_245_GC_PERCENT=50.000 PRIMER_LEFT_245_SELF_ANY=3.00 PRIMER_RIGHT_245_SELF_ANY=3.00 PRIMER_LEFT_245_SELF_END=0.00 PRIMER_RIGHT_245_SELF_END=2.00 PRIMER_LEFT_245_END_STABILITY=6.4000 PRIMER_RIGHT_245_END_STABILITY=10.2000 PRIMER_PAIR_245_COMPL_ANY=4.00 PRIMER_PAIR_245_COMPL_END=1.00 PRIMER_PAIR_245_PRODUCT_SIZE=97 PRIMER_PAIR_246_PENALTY=4.451941 PRIMER_LEFT_246_PENALTY=2.710504 PRIMER_RIGHT_246_PENALTY=1.741437 PRIMER_LEFT_246_SEQUENCE=TTTGGCACTGCTCCTGCT PRIMER_RIGHT_246_SEQUENCE=GAACATGGCAATCTGGGGT PRIMER_LEFT_246=211,18 PRIMER_RIGHT_246=310,19 PRIMER_LEFT_246_TM=60.711 PRIMER_RIGHT_246_TM=60.741 PRIMER_LEFT_246_GC_PERCENT=55.556 PRIMER_RIGHT_246_GC_PERCENT=52.632 PRIMER_LEFT_246_SELF_ANY=5.00 PRIMER_RIGHT_246_SELF_ANY=4.00 PRIMER_LEFT_246_SELF_END=0.00 PRIMER_RIGHT_246_SELF_END=0.00 PRIMER_LEFT_246_END_STABILITY=8.2000 PRIMER_RIGHT_246_END_STABILITY=10.6000 PRIMER_PAIR_246_COMPL_ANY=5.00 PRIMER_PAIR_246_COMPL_END=1.00 PRIMER_PAIR_246_PRODUCT_SIZE=100 PRIMER_PAIR_247_PENALTY=4.458236 PRIMER_LEFT_247_PENALTY=2.167720 PRIMER_RIGHT_247_PENALTY=2.290516 PRIMER_LEFT_247_SEQUENCE=CATCTTCACTGGCACACCGT PRIMER_RIGHT_247_SEQUENCE=AACTTGTCAATTCCGCAGG PRIMER_LEFT_247=638,20 PRIMER_RIGHT_247=735,19 PRIMER_LEFT_247_TM=62.168 PRIMER_RIGHT_247_TM=58.709 PRIMER_LEFT_247_GC_PERCENT=55.000 PRIMER_RIGHT_247_GC_PERCENT=47.368 PRIMER_LEFT_247_SELF_ANY=3.00 PRIMER_RIGHT_247_SELF_ANY=5.00 PRIMER_LEFT_247_SELF_END=3.00 PRIMER_RIGHT_247_SELF_END=2.00 PRIMER_LEFT_247_END_STABILITY=9.3000 PRIMER_RIGHT_247_END_STABILITY=9.7000 PRIMER_PAIR_247_COMPL_ANY=3.00 PRIMER_PAIR_247_COMPL_END=1.00 PRIMER_PAIR_247_PRODUCT_SIZE=98 PRIMER_PAIR_248_PENALTY=4.471214 PRIMER_LEFT_248_PENALTY=2.446999 PRIMER_RIGHT_248_PENALTY=2.024215 PRIMER_LEFT_248_SEQUENCE=AAGCCAACCAACCAGTGAC PRIMER_RIGHT_248_SEQUENCE=CCAACTAAGCAGCGGTAGGG PRIMER_LEFT_248=459,19 PRIMER_RIGHT_248=558,20 PRIMER_LEFT_248_TM=58.553 PRIMER_RIGHT_248_TM=62.024 PRIMER_LEFT_248_GC_PERCENT=52.632 PRIMER_RIGHT_248_GC_PERCENT=60.000 PRIMER_LEFT_248_SELF_ANY=3.00 PRIMER_RIGHT_248_SELF_ANY=3.00 PRIMER_LEFT_248_SELF_END=3.00 PRIMER_RIGHT_248_SELF_END=0.00 PRIMER_LEFT_248_END_STABILITY=6.1000 PRIMER_RIGHT_248_END_STABILITY=8.7000 PRIMER_PAIR_248_COMPL_ANY=4.00 PRIMER_PAIR_248_COMPL_END=2.00 PRIMER_PAIR_248_PRODUCT_SIZE=100 PRIMER_PAIR_249_PENALTY=4.476003 PRIMER_LEFT_249_PENALTY=2.738851 PRIMER_RIGHT_249_PENALTY=1.737152 PRIMER_LEFT_249_SEQUENCE=CAGACTGAACATGCACATGAA PRIMER_RIGHT_249_SEQUENCE=TGGTATCAATGCAGGTTTTGG PRIMER_LEFT_249=316,21 PRIMER_RIGHT_249=395,21 PRIMER_LEFT_249_TM=58.261 PRIMER_RIGHT_249_TM=60.737 PRIMER_LEFT_249_GC_PERCENT=42.857 PRIMER_RIGHT_249_GC_PERCENT=42.857 PRIMER_LEFT_249_SELF_ANY=6.00 PRIMER_RIGHT_249_SELF_ANY=4.00 PRIMER_LEFT_249_SELF_END=2.00 PRIMER_RIGHT_249_SELF_END=0.00 PRIMER_LEFT_249_END_STABILITY=6.9000 PRIMER_RIGHT_249_END_STABILITY=8.8000 PRIMER_PAIR_249_COMPL_ANY=6.00 PRIMER_PAIR_249_COMPL_END=3.00 PRIMER_PAIR_249_PRODUCT_SIZE=80 PRIMER_PAIR_250_PENALTY=4.535334 PRIMER_LEFT_250_PENALTY=2.240419 PRIMER_RIGHT_250_PENALTY=2.294916 PRIMER_LEFT_250_SEQUENCE=ACCGCTGCTTAGTTGGTGAGT PRIMER_RIGHT_250_SEQUENCE=AACATCCATCCTCTCCTGGTG PRIMER_LEFT_250=543,21 PRIMER_RIGHT_250=628,21 PRIMER_LEFT_250_TM=61.240 PRIMER_RIGHT_250_TM=61.295 PRIMER_LEFT_250_GC_PERCENT=52.381 PRIMER_RIGHT_250_GC_PERCENT=52.381 PRIMER_LEFT_250_SELF_ANY=3.00 PRIMER_RIGHT_250_SELF_ANY=3.00 PRIMER_LEFT_250_SELF_END=1.00 PRIMER_RIGHT_250_SELF_END=3.00 PRIMER_LEFT_250_END_STABILITY=6.4000 PRIMER_RIGHT_250_END_STABILITY=8.2000 PRIMER_PAIR_250_COMPL_ANY=4.00 PRIMER_PAIR_250_COMPL_END=0.00 PRIMER_PAIR_250_PRODUCT_SIZE=86 PRIMER_PAIR_251_PENALTY=4.550826 PRIMER_LEFT_251_PENALTY=3.135712 PRIMER_RIGHT_251_PENALTY=1.415114 PRIMER_LEFT_251_SEQUENCE=TATTGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_251_SEQUENCE=GTTCTGGATGGTCACTGGTTG PRIMER_LEFT_251=413,23 PRIMER_RIGHT_251=487,21 PRIMER_LEFT_251_TM=60.136 PRIMER_RIGHT_251_TM=60.415 PRIMER_LEFT_251_GC_PERCENT=43.478 PRIMER_RIGHT_251_GC_PERCENT=52.381 PRIMER_LEFT_251_SELF_ANY=5.00 PRIMER_RIGHT_251_SELF_ANY=3.00 PRIMER_LEFT_251_SELF_END=1.00 PRIMER_RIGHT_251_SELF_END=0.00 PRIMER_LEFT_251_END_STABILITY=8.2000 PRIMER_RIGHT_251_END_STABILITY=8.2000 PRIMER_PAIR_251_COMPL_ANY=4.00 PRIMER_PAIR_251_COMPL_END=3.00 PRIMER_PAIR_251_PRODUCT_SIZE=75 PRIMER_PAIR_252_PENALTY=4.564315 PRIMER_LEFT_252_PENALTY=2.887640 PRIMER_RIGHT_252_PENALTY=1.676675 PRIMER_LEFT_252_SEQUENCE=TTGCCATGTTCTGTGGCAG PRIMER_RIGHT_252_SEQUENCE=GGTCCCTGATGGATCTGAATC PRIMER_LEFT_252=300,19 PRIMER_RIGHT_252=376,21 PRIMER_LEFT_252_TM=61.888 PRIMER_RIGHT_252_TM=60.677 PRIMER_LEFT_252_GC_PERCENT=52.632 PRIMER_RIGHT_252_GC_PERCENT=52.381 PRIMER_LEFT_252_SELF_ANY=5.00 PRIMER_RIGHT_252_SELF_ANY=5.00 PRIMER_LEFT_252_SELF_END=3.00 PRIMER_RIGHT_252_SELF_END=3.00 PRIMER_LEFT_252_END_STABILITY=9.7000 PRIMER_RIGHT_252_END_STABILITY=6.6000 PRIMER_PAIR_252_COMPL_ANY=4.00 PRIMER_PAIR_252_COMPL_END=1.00 PRIMER_PAIR_252_PRODUCT_SIZE=77 PRIMER_PAIR_253_PENALTY=4.571775 PRIMER_LEFT_253_PENALTY=2.444325 PRIMER_RIGHT_253_PENALTY=2.127449 PRIMER_LEFT_253_SEQUENCE=GGTAGAAGCCAACCAACCA PRIMER_RIGHT_253_SEQUENCE=CACAAAGTGGGGATGGGTCT PRIMER_LEFT_253=454,19 PRIMER_RIGHT_253=535,20 PRIMER_LEFT_253_TM=58.556 PRIMER_RIGHT_253_TM=62.127 PRIMER_LEFT_253_GC_PERCENT=52.632 PRIMER_RIGHT_253_GC_PERCENT=55.000 PRIMER_LEFT_253_SELF_ANY=3.00 PRIMER_RIGHT_253_SELF_ANY=3.00 PRIMER_LEFT_253_SELF_END=0.00 PRIMER_RIGHT_253_SELF_END=0.00 PRIMER_LEFT_253_END_STABILITY=8.2000 PRIMER_RIGHT_253_END_STABILITY=7.6000 PRIMER_PAIR_253_COMPL_ANY=4.00 PRIMER_PAIR_253_COMPL_END=2.00 PRIMER_PAIR_253_PRODUCT_SIZE=82 PRIMER_PAIR_254_PENALTY=4.592141 PRIMER_LEFT_254_PENALTY=3.239726 PRIMER_RIGHT_254_PENALTY=1.352415 PRIMER_LEFT_254_SEQUENCE=GCATTGATACCAAGGAAGGCA PRIMER_RIGHT_254_SEQUENCE=CACTGGTTGGTTGGCTTCTAC PRIMER_LEFT_254=384,21 PRIMER_RIGHT_254=475,21 PRIMER_LEFT_254_TM=62.240 PRIMER_RIGHT_254_TM=59.648 PRIMER_LEFT_254_GC_PERCENT=47.619 PRIMER_RIGHT_254_GC_PERCENT=52.381 PRIMER_LEFT_254_SELF_ANY=6.00 PRIMER_RIGHT_254_SELF_ANY=3.00 PRIMER_LEFT_254_SELF_END=0.00 PRIMER_RIGHT_254_SELF_END=0.00 PRIMER_LEFT_254_END_STABILITY=9.7000 PRIMER_RIGHT_254_END_STABILITY=5.4000 PRIMER_PAIR_254_COMPL_ANY=5.00 PRIMER_PAIR_254_COMPL_END=2.00 PRIMER_PAIR_254_PRODUCT_SIZE=92 PRIMER_PAIR_255_PENALTY=4.598043 PRIMER_LEFT_255_PENALTY=2.137489 PRIMER_RIGHT_255_PENALTY=2.460554 PRIMER_LEFT_255_SEQUENCE=GTAAGTGATGCCCTTCTCGTTC PRIMER_RIGHT_255_SEQUENCE=CAGTGAAGATGAGTTTCGCA PRIMER_LEFT_255=566,22 PRIMER_RIGHT_255=648,20 PRIMER_LEFT_255_TM=60.137 PRIMER_RIGHT_255_TM=57.539 PRIMER_LEFT_255_GC_PERCENT=50.000 PRIMER_RIGHT_255_GC_PERCENT=45.000 PRIMER_LEFT_255_SELF_ANY=4.00 PRIMER_RIGHT_255_SELF_ANY=3.00 PRIMER_LEFT_255_SELF_END=2.00 PRIMER_RIGHT_255_SELF_END=2.00 PRIMER_LEFT_255_END_STABILITY=8.4000 PRIMER_RIGHT_255_END_STABILITY=10.2000 PRIMER_PAIR_255_COMPL_ANY=4.00 PRIMER_PAIR_255_COMPL_END=1.00 PRIMER_PAIR_255_PRODUCT_SIZE=83 PRIMER_PAIR_256_PENALTY=4.636635 PRIMER_LEFT_256_PENALTY=2.444325 PRIMER_RIGHT_256_PENALTY=2.192310 PRIMER_LEFT_256_SEQUENCE=TGGTAGAAGCCAACCAACC PRIMER_RIGHT_256_SEQUENCE=CACAAAGTGGGGATGGGTC PRIMER_LEFT_256=453,19 PRIMER_RIGHT_256=535,19 PRIMER_LEFT_256_TM=58.556 PRIMER_RIGHT_256_TM=61.192 PRIMER_LEFT_256_GC_PERCENT=52.632 PRIMER_RIGHT_256_GC_PERCENT=57.895 PRIMER_LEFT_256_SELF_ANY=4.00 PRIMER_RIGHT_256_SELF_ANY=3.00 PRIMER_LEFT_256_SELF_END=0.00 PRIMER_RIGHT_256_SELF_END=1.00 PRIMER_LEFT_256_END_STABILITY=8.2000 PRIMER_RIGHT_256_END_STABILITY=9.1000 PRIMER_PAIR_256_COMPL_ANY=4.00 PRIMER_PAIR_256_COMPL_END=3.00 PRIMER_PAIR_256_PRODUCT_SIZE=83 PRIMER_PAIR_257_PENALTY=4.644527 PRIMER_LEFT_257_PENALTY=2.328173 PRIMER_RIGHT_257_PENALTY=2.316355 PRIMER_LEFT_257_SEQUENCE=CATCTTCACTGGCACACCG PRIMER_RIGHT_257_SEQUENCE=AACTTGTCAATTCCGCAGGG PRIMER_LEFT_257=638,19 PRIMER_RIGHT_257=735,20 PRIMER_LEFT_257_TM=61.328 PRIMER_RIGHT_257_TM=62.316 PRIMER_LEFT_257_GC_PERCENT=57.895 PRIMER_RIGHT_257_GC_PERCENT=50.000 PRIMER_LEFT_257_SELF_ANY=3.00 PRIMER_RIGHT_257_SELF_ANY=5.00 PRIMER_LEFT_257_SELF_END=2.00 PRIMER_RIGHT_257_SELF_END=1.00 PRIMER_LEFT_257_END_STABILITY=9.9000 PRIMER_RIGHT_257_END_STABILITY=9.7000 PRIMER_PAIR_257_COMPL_ANY=3.00 PRIMER_PAIR_257_COMPL_END=1.00 PRIMER_PAIR_257_PRODUCT_SIZE=98 PRIMER_PAIR_258_PENALTY=4.660248 PRIMER_LEFT_258_PENALTY=2.477524 PRIMER_RIGHT_258_PENALTY=2.182723 PRIMER_LEFT_258_SEQUENCE=TCACCAATGTGGTAGAAGCCA PRIMER_RIGHT_258_SEQUENCE=GATGGGTCTTGCACTGCTT PRIMER_LEFT_258=444,21 PRIMER_RIGHT_258=524,19 PRIMER_LEFT_258_TM=61.478 PRIMER_RIGHT_258_TM=58.817 PRIMER_LEFT_258_GC_PERCENT=47.619 PRIMER_RIGHT_258_GC_PERCENT=52.632 PRIMER_LEFT_258_SELF_ANY=6.00 PRIMER_RIGHT_258_SELF_ANY=5.00 PRIMER_LEFT_258_SELF_END=1.00 PRIMER_RIGHT_258_SELF_END=1.00 PRIMER_LEFT_258_END_STABILITY=9.7000 PRIMER_RIGHT_258_END_STABILITY=8.5000 PRIMER_PAIR_258_COMPL_ANY=4.00 PRIMER_PAIR_258_COMPL_END=3.00 PRIMER_PAIR_258_PRODUCT_SIZE=81 PRIMER_PAIR_259_PENALTY=4.664552 PRIMER_LEFT_259_PENALTY=2.305331 PRIMER_RIGHT_259_PENALTY=2.359221 PRIMER_LEFT_259_SEQUENCE=CCCATCCCCACTTTGTGA PRIMER_RIGHT_259_SEQUENCE=TGTCAGGAACGAGAAGGGC PRIMER_LEFT_259=519,18 PRIMER_RIGHT_259=593,19 PRIMER_LEFT_259_TM=60.305 PRIMER_RIGHT_259_TM=61.359 PRIMER_LEFT_259_GC_PERCENT=55.556 PRIMER_RIGHT_259_GC_PERCENT=57.895 PRIMER_LEFT_259_SELF_ANY=3.00 PRIMER_RIGHT_259_SELF_ANY=3.00 PRIMER_LEFT_259_SELF_END=2.00 PRIMER_RIGHT_259_SELF_END=2.00 PRIMER_LEFT_259_END_STABILITY=6.7000 PRIMER_RIGHT_259_END_STABILITY=10.9000 PRIMER_PAIR_259_COMPL_ANY=4.00 PRIMER_PAIR_259_COMPL_END=2.00 PRIMER_PAIR_259_PRODUCT_SIZE=75 PRIMER_PAIR_260_PENALTY=4.684530 PRIMER_LEFT_260_PENALTY=2.282418 PRIMER_RIGHT_260_PENALTY=2.402111 PRIMER_LEFT_260_SEQUENCE=TGATGCCCTTCTCGTTCC PRIMER_RIGHT_260_SEQUENCE=GTCTCTTTGGCGACGGTG PRIMER_LEFT_260=571,18 PRIMER_RIGHT_260=669,18 PRIMER_LEFT_260_TM=59.718 PRIMER_RIGHT_260_TM=60.402 PRIMER_LEFT_260_GC_PERCENT=55.556 PRIMER_RIGHT_260_GC_PERCENT=61.111 PRIMER_LEFT_260_SELF_ANY=2.00 PRIMER_RIGHT_260_SELF_ANY=3.00 PRIMER_LEFT_260_SELF_END=0.00 PRIMER_RIGHT_260_SELF_END=1.00 PRIMER_LEFT_260_END_STABILITY=7.9000 PRIMER_RIGHT_260_END_STABILITY=9.9000 PRIMER_PAIR_260_COMPL_ANY=3.00 PRIMER_PAIR_260_COMPL_END=1.00 PRIMER_PAIR_260_PRODUCT_SIZE=99 PRIMER_PAIR_261_PENALTY=4.687539 PRIMER_LEFT_261_PENALTY=2.495229 PRIMER_RIGHT_261_PENALTY=2.192310 PRIMER_LEFT_261_SEQUENCE=AGAAGCCAACCAACCAGTGAC PRIMER_RIGHT_261_SEQUENCE=TCACAAAGTGGGGATGGGT PRIMER_LEFT_261=457,21 PRIMER_RIGHT_261=536,19 PRIMER_LEFT_261_TM=61.495 PRIMER_RIGHT_261_TM=61.192 PRIMER_LEFT_261_GC_PERCENT=52.381 PRIMER_RIGHT_261_GC_PERCENT=52.632 PRIMER_LEFT_261_SELF_ANY=3.00 PRIMER_RIGHT_261_SELF_ANY=3.00 PRIMER_LEFT_261_SELF_END=3.00 PRIMER_RIGHT_261_SELF_END=0.00 PRIMER_LEFT_261_END_STABILITY=6.1000 PRIMER_RIGHT_261_END_STABILITY=9.4000 PRIMER_PAIR_261_COMPL_ANY=4.00 PRIMER_PAIR_261_COMPL_END=3.00 PRIMER_PAIR_261_PRODUCT_SIZE=80 PRIMER_PAIR_262_PENALTY=4.704795 PRIMER_LEFT_262_PENALTY=2.567306 PRIMER_RIGHT_262_PENALTY=2.137489 PRIMER_LEFT_262_SEQUENCE=GCAAGCAGTGCAAGACCC PRIMER_RIGHT_262_SEQUENCE=GAACGAGAAGGGCATCACTTAC PRIMER_LEFT_262=504,18 PRIMER_RIGHT_262=587,22 PRIMER_LEFT_262_TM=60.567 PRIMER_RIGHT_262_TM=60.137 PRIMER_LEFT_262_GC_PERCENT=61.111 PRIMER_RIGHT_262_GC_PERCENT=50.000 PRIMER_LEFT_262_SELF_ANY=5.00 PRIMER_RIGHT_262_SELF_ANY=4.00 PRIMER_LEFT_262_SELF_END=0.00 PRIMER_RIGHT_262_SELF_END=2.00 PRIMER_LEFT_262_END_STABILITY=9.1000 PRIMER_RIGHT_262_END_STABILITY=5.7000 PRIMER_PAIR_262_COMPL_ANY=4.00 PRIMER_PAIR_262_COMPL_END=2.00 PRIMER_PAIR_262_PRODUCT_SIZE=84 PRIMER_PAIR_263_PENALTY=4.716875 PRIMER_LEFT_263_PENALTY=3.088710 PRIMER_RIGHT_263_PENALTY=1.628165 PRIMER_LEFT_263_SEQUENCE=ACCCACTGATGGTAATGCTGG PRIMER_RIGHT_263_SEQUENCE=ATCCCACTTCCCATTCTGGA PRIMER_LEFT_263=259,21 PRIMER_RIGHT_263=358,20 PRIMER_LEFT_263_TM=62.089 PRIMER_RIGHT_263_TM=61.628 PRIMER_LEFT_263_GC_PERCENT=52.381 PRIMER_RIGHT_263_GC_PERCENT=50.000 PRIMER_LEFT_263_SELF_ANY=3.00 PRIMER_RIGHT_263_SELF_ANY=3.00 PRIMER_LEFT_263_SELF_END=0.00 PRIMER_RIGHT_263_SELF_END=2.00 PRIMER_LEFT_263_END_STABILITY=9.7000 PRIMER_RIGHT_263_END_STABILITY=8.2000 PRIMER_PAIR_263_COMPL_ANY=5.00 PRIMER_PAIR_263_COMPL_END=3.00 PRIMER_PAIR_263_PRODUCT_SIZE=100 PRIMER_PAIR_264_PENALTY=4.761332 PRIMER_LEFT_264_PENALTY=2.359221 PRIMER_RIGHT_264_PENALTY=2.402111 PRIMER_LEFT_264_SEQUENCE=GCCCTTCTCGTTCCTGACA PRIMER_RIGHT_264_SEQUENCE=TGTCTCTTTGGCGACGGT PRIMER_LEFT_264=575,19 PRIMER_RIGHT_264=670,18 PRIMER_LEFT_264_TM=61.359 PRIMER_RIGHT_264_TM=60.402 PRIMER_LEFT_264_GC_PERCENT=57.895 PRIMER_RIGHT_264_GC_PERCENT=55.556 PRIMER_LEFT_264_SELF_ANY=3.00 PRIMER_RIGHT_264_SELF_ANY=3.00 PRIMER_LEFT_264_SELF_END=3.00 PRIMER_RIGHT_264_SELF_END=3.00 PRIMER_LEFT_264_END_STABILITY=6.7000 PRIMER_RIGHT_264_END_STABILITY=9.3000 PRIMER_PAIR_264_COMPL_ANY=4.00 PRIMER_PAIR_264_COMPL_END=1.00 PRIMER_PAIR_264_PRODUCT_SIZE=96 PRIMER_PAIR_265_PENALTY=4.766661 PRIMER_LEFT_265_PENALTY=2.785923 PRIMER_RIGHT_265_PENALTY=1.980738 PRIMER_LEFT_265_SEQUENCE=CATGCACATGAATGTCCAGAAT PRIMER_RIGHT_265_SEQUENCE=TCCTTGGTATCAATGCAGGT PRIMER_LEFT_265=325,22 PRIMER_RIGHT_265=399,20 PRIMER_LEFT_265_TM=60.786 PRIMER_RIGHT_265_TM=58.019 PRIMER_LEFT_265_GC_PERCENT=40.909 PRIMER_RIGHT_265_GC_PERCENT=45.000 PRIMER_LEFT_265_SELF_ANY=8.00 PRIMER_RIGHT_265_SELF_ANY=6.00 PRIMER_LEFT_265_SELF_END=2.00 PRIMER_RIGHT_265_SELF_END=0.00 PRIMER_LEFT_265_END_STABILITY=6.6000 PRIMER_RIGHT_265_END_STABILITY=7.9000 PRIMER_PAIR_265_COMPL_ANY=4.00 PRIMER_PAIR_265_COMPL_END=2.00 PRIMER_PAIR_265_PRODUCT_SIZE=75 PRIMER_PAIR_266_PENALTY=4.797870 PRIMER_LEFT_266_PENALTY=2.668729 PRIMER_RIGHT_266_PENALTY=2.129141 PRIMER_LEFT_266_SEQUENCE=ATTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_266_SEQUENCE=GCACCAGTTCTGGATGGTCA PRIMER_LEFT_266=414,21 PRIMER_RIGHT_266=493,20 PRIMER_LEFT_266_TM=58.331 PRIMER_RIGHT_266_TM=62.129 PRIMER_LEFT_266_GC_PERCENT=47.619 PRIMER_RIGHT_266_GC_PERCENT=55.000 PRIMER_LEFT_266_SELF_ANY=5.00 PRIMER_RIGHT_266_SELF_ANY=6.00 PRIMER_LEFT_266_SELF_END=1.00 PRIMER_RIGHT_266_SELF_END=2.00 PRIMER_LEFT_266_END_STABILITY=9.7000 PRIMER_RIGHT_266_END_STABILITY=7.9000 PRIMER_PAIR_266_COMPL_ANY=4.00 PRIMER_PAIR_266_COMPL_END=2.00 PRIMER_PAIR_266_PRODUCT_SIZE=80 PRIMER_PAIR_267_PENALTY=4.805322 PRIMER_LEFT_267_PENALTY=1.014189 PRIMER_RIGHT_267_PENALTY=3.791133 PRIMER_LEFT_267_SEQUENCE=TCAGATCCATCAGGGACCA PRIMER_RIGHT_267_SEQUENCE=CAGGGTAGACTTCTTGGCAATAC PRIMER_LEFT_267=359,19 PRIMER_RIGHT_267=434,23 PRIMER_LEFT_267_TM=59.986 PRIMER_RIGHT_267_TM=59.209 PRIMER_LEFT_267_GC_PERCENT=52.632 PRIMER_RIGHT_267_GC_PERCENT=47.826 PRIMER_LEFT_267_SELF_ANY=5.00 PRIMER_RIGHT_267_SELF_ANY=5.00 PRIMER_LEFT_267_SELF_END=3.00 PRIMER_RIGHT_267_SELF_END=3.00 PRIMER_LEFT_267_END_STABILITY=7.9000 PRIMER_RIGHT_267_END_STABILITY=5.6000 PRIMER_PAIR_267_COMPL_ANY=3.00 PRIMER_PAIR_267_COMPL_END=0.00 PRIMER_PAIR_267_PRODUCT_SIZE=76 PRIMER_PAIR_268_PENALTY=4.817718 PRIMER_LEFT_268_PENALTY=4.397076 PRIMER_RIGHT_268_PENALTY=0.420643 PRIMER_LEFT_268_SEQUENCE=CGGTTTGGCACTGCTCCT PRIMER_RIGHT_268_SEQUENCE=AGGCCAGCATTACCATCAGT PRIMER_LEFT_268=208,18 PRIMER_RIGHT_268=282,20 PRIMER_LEFT_268_TM=62.397 PRIMER_RIGHT_268_TM=59.579 PRIMER_LEFT_268_GC_PERCENT=61.111 PRIMER_RIGHT_268_GC_PERCENT=50.000 PRIMER_LEFT_268_SELF_ANY=5.00 PRIMER_RIGHT_268_SELF_ANY=4.00 PRIMER_LEFT_268_SELF_END=0.00 PRIMER_RIGHT_268_SELF_END=1.00 PRIMER_LEFT_268_END_STABILITY=7.9000 PRIMER_RIGHT_268_END_STABILITY=6.4000 PRIMER_PAIR_268_COMPL_ANY=6.00 PRIMER_PAIR_268_COMPL_END=3.00 PRIMER_PAIR_268_PRODUCT_SIZE=75 PRIMER_PAIR_269_PENALTY=4.818613 PRIMER_LEFT_269_PENALTY=3.329319 PRIMER_RIGHT_269_PENALTY=1.489294 PRIMER_LEFT_269_SEQUENCE=CAAAGAGACATGCAGTGAGAAGA PRIMER_RIGHT_269_SEQUENCE=GCAACACACAAACTCTACCCC PRIMER_LEFT_269=661,23 PRIMER_RIGHT_269=760,21 PRIMER_LEFT_269_TM=59.671 PRIMER_RIGHT_269_TM=59.511 PRIMER_LEFT_269_GC_PERCENT=43.478 PRIMER_RIGHT_269_GC_PERCENT=52.381 PRIMER_LEFT_269_SELF_ANY=4.00 PRIMER_RIGHT_269_SELF_ANY=2.00 PRIMER_LEFT_269_SELF_END=0.00 PRIMER_RIGHT_269_SELF_END=0.00 PRIMER_LEFT_269_END_STABILITY=6.7000 PRIMER_RIGHT_269_END_STABILITY=10.6000 PRIMER_PAIR_269_COMPL_ANY=4.00 PRIMER_PAIR_269_COMPL_END=2.00 PRIMER_PAIR_269_PRODUCT_SIZE=100 PRIMER_PAIR_270_PENALTY=4.849721 PRIMER_LEFT_270_PENALTY=3.200839 PRIMER_RIGHT_270_PENALTY=1.648882 PRIMER_LEFT_270_SEQUENCE=GCTGGCTGAACCCCAGAT PRIMER_RIGHT_270_SEQUENCE=TGATGGATCTGAATCCCACTT PRIMER_LEFT_270=283,18 PRIMER_RIGHT_270=370,21 PRIMER_LEFT_270_TM=61.201 PRIMER_RIGHT_270_TM=59.351 PRIMER_LEFT_270_GC_PERCENT=61.111 PRIMER_RIGHT_270_GC_PERCENT=42.857 PRIMER_LEFT_270_SELF_ANY=4.00 PRIMER_RIGHT_270_SELF_ANY=5.00 PRIMER_LEFT_270_SELF_END=2.00 PRIMER_RIGHT_270_SELF_END=0.00 PRIMER_LEFT_270_END_STABILITY=6.6000 PRIMER_RIGHT_270_END_STABILITY=6.7000 PRIMER_PAIR_270_COMPL_ANY=5.00 PRIMER_PAIR_270_COMPL_END=0.00 PRIMER_PAIR_270_PRODUCT_SIZE=88 PRIMER_PAIR_271_PENALTY=4.881052 PRIMER_LEFT_271_PENALTY=1.081589 PRIMER_RIGHT_271_PENALTY=3.799463 PRIMER_LEFT_271_SEQUENCE=ATCAGGGACCAAAACCTGC PRIMER_RIGHT_271_SEQUENCE=GCTTCTACCACATTGGTGATCT PRIMER_LEFT_271=367,19 PRIMER_RIGHT_271=462,22 PRIMER_LEFT_271_TM=59.918 PRIMER_RIGHT_271_TM=58.201 PRIMER_LEFT_271_GC_PERCENT=52.632 PRIMER_RIGHT_271_GC_PERCENT=45.455 PRIMER_LEFT_271_SELF_ANY=4.00 PRIMER_RIGHT_271_SELF_ANY=6.00 PRIMER_LEFT_271_SELF_END=2.00 PRIMER_RIGHT_271_SELF_END=2.00 PRIMER_LEFT_271_END_STABILITY=9.7000 PRIMER_RIGHT_271_END_STABILITY=6.3000 PRIMER_PAIR_271_COMPL_ANY=5.00 PRIMER_PAIR_271_COMPL_END=0.00 PRIMER_PAIR_271_PRODUCT_SIZE=96 PRIMER_PAIR_272_PENALTY=4.883797 PRIMER_LEFT_272_PENALTY=2.601441 PRIMER_RIGHT_272_PENALTY=2.282356 PRIMER_LEFT_272_SEQUENCE=GACCAAAACCTGCATTGATACC PRIMER_RIGHT_272_SEQUENCE=GTTGGTTGGCTTCTACCACATT PRIMER_LEFT_272=373,22 PRIMER_RIGHT_272=470,22 PRIMER_LEFT_272_TM=60.601 PRIMER_RIGHT_272_TM=60.282 PRIMER_LEFT_272_GC_PERCENT=45.455 PRIMER_RIGHT_272_GC_PERCENT=45.455 PRIMER_LEFT_272_SELF_ANY=4.00 PRIMER_RIGHT_272_SELF_ANY=4.00 PRIMER_LEFT_272_SELF_END=2.00 PRIMER_RIGHT_272_SELF_END=1.00 PRIMER_LEFT_272_END_STABILITY=6.8000 PRIMER_RIGHT_272_END_STABILITY=6.6000 PRIMER_PAIR_272_COMPL_ANY=5.00 PRIMER_PAIR_272_COMPL_END=1.00 PRIMER_PAIR_272_PRODUCT_SIZE=98 PRIMER_PAIR_273_PENALTY=4.895033 PRIMER_LEFT_273_PENALTY=2.612614 PRIMER_RIGHT_273_PENALTY=2.282418 PRIMER_LEFT_273_SEQUENCE=CAAGACCCATCCCCACTTTG PRIMER_RIGHT_273_SEQUENCE=GGAACGAGAAGGGCATCA PRIMER_LEFT_273=514,20 PRIMER_RIGHT_273=588,18 PRIMER_LEFT_273_TM=62.613 PRIMER_RIGHT_273_TM=59.718 PRIMER_LEFT_273_GC_PERCENT=55.000 PRIMER_RIGHT_273_GC_PERCENT=55.556 PRIMER_LEFT_273_SELF_ANY=3.00 PRIMER_RIGHT_273_SELF_ANY=2.00 PRIMER_LEFT_273_SELF_END=1.00 PRIMER_RIGHT_273_SELF_END=1.00 PRIMER_LEFT_273_END_STABILITY=7.3000 PRIMER_RIGHT_273_END_STABILITY=6.9000 PRIMER_PAIR_273_COMPL_ANY=4.00 PRIMER_PAIR_273_COMPL_END=2.00 PRIMER_PAIR_273_PRODUCT_SIZE=75 PRIMER_PAIR_274_PENALTY=4.900206 PRIMER_LEFT_274_PENALTY=2.653319 PRIMER_RIGHT_274_PENALTY=2.246886 PRIMER_LEFT_274_SEQUENCE=CCAATGTGGTAGAAGCCAACC PRIMER_RIGHT_274_SEQUENCE=GGATGGGTCTTGCACTGCT PRIMER_LEFT_274=447,21 PRIMER_RIGHT_274=525,19 PRIMER_LEFT_274_TM=61.653 PRIMER_RIGHT_274_TM=61.247 PRIMER_LEFT_274_GC_PERCENT=52.381 PRIMER_RIGHT_274_GC_PERCENT=57.895 PRIMER_LEFT_274_SELF_ANY=4.00 PRIMER_RIGHT_274_SELF_ANY=5.00 PRIMER_LEFT_274_SELF_END=2.00 PRIMER_RIGHT_274_SELF_END=3.00 PRIMER_LEFT_274_END_STABILITY=8.2000 PRIMER_RIGHT_274_END_STABILITY=8.2000 PRIMER_PAIR_274_COMPL_ANY=5.00 PRIMER_PAIR_274_COMPL_END=2.00 PRIMER_PAIR_274_PRODUCT_SIZE=79 PRIMER_PAIR_275_PENALTY=4.913597 PRIMER_LEFT_275_PENALTY=3.239726 PRIMER_RIGHT_275_PENALTY=1.673871 PRIMER_LEFT_275_SEQUENCE=TGCATTGATACCAAGGAAGGC PRIMER_RIGHT_275_SEQUENCE=TCACTGGTTGGTTGGCTTCTA PRIMER_LEFT_275=383,21 PRIMER_RIGHT_275=476,21 PRIMER_LEFT_275_TM=62.240 PRIMER_RIGHT_275_TM=60.674 PRIMER_LEFT_275_GC_PERCENT=47.619 PRIMER_RIGHT_275_GC_PERCENT=47.619 PRIMER_LEFT_275_SELF_ANY=6.00 PRIMER_RIGHT_275_SELF_ANY=3.00 PRIMER_LEFT_275_SELF_END=2.00 PRIMER_RIGHT_275_SELF_END=2.00 PRIMER_LEFT_275_END_STABILITY=9.7000 PRIMER_RIGHT_275_END_STABILITY=6.0000 PRIMER_PAIR_275_COMPL_ANY=5.00 PRIMER_PAIR_275_COMPL_END=2.00 PRIMER_PAIR_275_PRODUCT_SIZE=94 PRIMER_PAIR_276_PENALTY=4.919069 PRIMER_LEFT_276_PENALTY=2.487583 PRIMER_RIGHT_276_PENALTY=2.431486 PRIMER_LEFT_276_SEQUENCE=TTCTTACACCAGGAGAGGATGG PRIMER_RIGHT_276_SEQUENCE=TTCTCACTGCATGTCTCTTTGG PRIMER_LEFT_276=602,22 PRIMER_RIGHT_276=681,22 PRIMER_LEFT_276_TM=60.488 PRIMER_RIGHT_276_TM=60.431 PRIMER_LEFT_276_GC_PERCENT=50.000 PRIMER_RIGHT_276_GC_PERCENT=45.455 PRIMER_LEFT_276_SELF_ANY=3.00 PRIMER_RIGHT_276_SELF_ANY=4.00 PRIMER_LEFT_276_SELF_END=0.00 PRIMER_RIGHT_276_SELF_END=0.00 PRIMER_LEFT_276_END_STABILITY=8.1000 PRIMER_RIGHT_276_END_STABILITY=8.8000 PRIMER_PAIR_276_COMPL_ANY=5.00 PRIMER_PAIR_276_COMPL_END=3.00 PRIMER_PAIR_276_PRODUCT_SIZE=80 PRIMER_PAIR_277_PENALTY=4.925479 PRIMER_LEFT_277_PENALTY=1.614650 PRIMER_RIGHT_277_PENALTY=3.310829 PRIMER_LEFT_277_SEQUENCE=ACCAGGAGAGGATGGATGTT PRIMER_RIGHT_277_SEQUENCE=CCGTAGTCATGCAAGTTGG PRIMER_LEFT_277=609,20 PRIMER_RIGHT_277=705,19 PRIMER_LEFT_277_TM=58.385 PRIMER_RIGHT_277_TM=57.689 PRIMER_LEFT_277_GC_PERCENT=50.000 PRIMER_RIGHT_277_GC_PERCENT=52.632 PRIMER_LEFT_277_SELF_ANY=3.00 PRIMER_RIGHT_277_SELF_ANY=5.00 PRIMER_LEFT_277_SELF_END=0.00 PRIMER_RIGHT_277_SELF_END=3.00 PRIMER_LEFT_277_END_STABILITY=6.6000 PRIMER_RIGHT_277_END_STABILITY=8.2000 PRIMER_PAIR_277_COMPL_ANY=4.00 PRIMER_PAIR_277_COMPL_END=0.00 PRIMER_PAIR_277_PRODUCT_SIZE=97 PRIMER_PAIR_278_PENALTY=4.973196 PRIMER_LEFT_278_PENALTY=1.147302 PRIMER_RIGHT_278_PENALTY=3.825895 PRIMER_LEFT_278_SEQUENCE=GGAAGTGGGATTCAGATCCAT PRIMER_RIGHT_278_SEQUENCE=TGCAGTTCAGGGTAGACTTCTTG PRIMER_LEFT_278=348,21 PRIMER_RIGHT_278=441,23 PRIMER_LEFT_278_TM=60.147 PRIMER_RIGHT_278_TM=60.826 PRIMER_LEFT_278_GC_PERCENT=47.619 PRIMER_RIGHT_278_GC_PERCENT=47.826 PRIMER_LEFT_278_SELF_ANY=5.00 PRIMER_RIGHT_278_SELF_ANY=4.00 PRIMER_LEFT_278_SELF_END=2.00 PRIMER_RIGHT_278_SELF_END=1.00 PRIMER_LEFT_278_END_STABILITY=8.1000 PRIMER_RIGHT_278_END_STABILITY=7.0000 PRIMER_PAIR_278_COMPL_ANY=5.00 PRIMER_PAIR_278_COMPL_END=1.00 PRIMER_PAIR_278_PRODUCT_SIZE=94 PRIMER_PAIR_279_PENALTY=4.998681 PRIMER_LEFT_279_PENALTY=2.459929 PRIMER_RIGHT_279_PENALTY=2.538752 PRIMER_LEFT_279_SEQUENCE=CCTACCGCTGCTTAGTTGG PRIMER_RIGHT_279_SEQUENCE=ATCCATCCTCTCCTGGTGTAAG PRIMER_LEFT_279=540,19 PRIMER_RIGHT_279=625,22 PRIMER_LEFT_279_TM=58.540 PRIMER_RIGHT_279_TM=59.461 PRIMER_LEFT_279_GC_PERCENT=57.895 PRIMER_RIGHT_279_GC_PERCENT=50.000 PRIMER_LEFT_279_SELF_ANY=3.00 PRIMER_RIGHT_279_SELF_ANY=3.00 PRIMER_LEFT_279_SELF_END=0.00 PRIMER_RIGHT_279_SELF_END=1.00 PRIMER_LEFT_279_END_STABILITY=8.2000 PRIMER_RIGHT_279_END_STABILITY=5.7000 PRIMER_PAIR_279_COMPL_ANY=4.00 PRIMER_PAIR_279_COMPL_END=1.00 PRIMER_PAIR_279_PRODUCT_SIZE=86 PRIMER_PAIR_280_PENALTY=5.000320 PRIMER_LEFT_280_PENALTY=2.459929 PRIMER_RIGHT_280_PENALTY=2.540392 PRIMER_LEFT_280_SEQUENCE=CCCTACCGCTGCTTAGTTG PRIMER_RIGHT_280_SEQUENCE=ATCCATCCTCTCCTGGTGTAA PRIMER_LEFT_280=539,19 PRIMER_RIGHT_280=625,21 PRIMER_LEFT_280_TM=58.540 PRIMER_RIGHT_280_TM=58.460 PRIMER_LEFT_280_GC_PERCENT=57.895 PRIMER_RIGHT_280_GC_PERCENT=47.619 PRIMER_LEFT_280_SELF_ANY=3.00 PRIMER_RIGHT_280_SELF_ANY=3.00 PRIMER_LEFT_280_SELF_END=1.00 PRIMER_RIGHT_280_SELF_END=1.00 PRIMER_LEFT_280_END_STABILITY=6.7000 PRIMER_RIGHT_280_END_STABILITY=6.0000 PRIMER_PAIR_280_COMPL_ANY=3.00 PRIMER_PAIR_280_COMPL_END=1.00 PRIMER_PAIR_280_PRODUCT_SIZE=87 PRIMER_PAIR_281_PENALTY=5.002464 PRIMER_LEFT_281_PENALTY=2.359221 PRIMER_RIGHT_281_PENALTY=2.643243 PRIMER_LEFT_281_SEQUENCE=TGCCCTTCTCGTTCCTGAC PRIMER_RIGHT_281_SEQUENCE=TGTGCCAGTGAAGATGAGTTT PRIMER_LEFT_281=574,19 PRIMER_RIGHT_281=653,21 PRIMER_LEFT_281_TM=61.359 PRIMER_RIGHT_281_TM=58.357 PRIMER_LEFT_281_GC_PERCENT=57.895 PRIMER_RIGHT_281_GC_PERCENT=42.857 PRIMER_LEFT_281_SELF_ANY=2.00 PRIMER_RIGHT_281_SELF_ANY=3.00 PRIMER_LEFT_281_SELF_END=1.00 PRIMER_RIGHT_281_SELF_END=1.00 PRIMER_LEFT_281_END_STABILITY=6.4000 PRIMER_RIGHT_281_END_STABILITY=6.7000 PRIMER_PAIR_281_COMPL_ANY=4.00 PRIMER_PAIR_281_COMPL_END=1.00 PRIMER_PAIR_281_PRODUCT_SIZE=80 PRIMER_PAIR_282_PENALTY=5.018265 PRIMER_LEFT_282_PENALTY=1.604487 PRIMER_RIGHT_282_PENALTY=3.413779 PRIMER_LEFT_282_SEQUENCE=TGAATGTCCAGAATGGGAAGT PRIMER_RIGHT_282_SEQUENCE=CAGGATGCCTTCCTTGGT PRIMER_LEFT_282=333,21 PRIMER_RIGHT_282=409,18 PRIMER_LEFT_282_TM=59.396 PRIMER_RIGHT_282_TM=58.586 PRIMER_LEFT_282_GC_PERCENT=42.857 PRIMER_RIGHT_282_GC_PERCENT=55.556 PRIMER_LEFT_282_SELF_ANY=3.00 PRIMER_RIGHT_282_SELF_ANY=7.00 PRIMER_LEFT_282_SELF_END=1.00 PRIMER_RIGHT_282_SELF_END=0.00 PRIMER_LEFT_282_END_STABILITY=6.4000 PRIMER_RIGHT_282_END_STABILITY=8.2000 PRIMER_PAIR_282_COMPL_ANY=5.00 PRIMER_PAIR_282_COMPL_END=1.00 PRIMER_PAIR_282_PRODUCT_SIZE=77 PRIMER_PAIR_283_PENALTY=5.045956 PRIMER_LEFT_283_PENALTY=2.895805 PRIMER_RIGHT_283_PENALTY=2.150151 PRIMER_LEFT_283_SEQUENCE=GGAGGTACCCACTGATGGTAAT PRIMER_RIGHT_283_SEQUENCE=CTTCCCATTCTGGACATTCAT PRIMER_LEFT_283=253,22 PRIMER_RIGHT_283=352,21 PRIMER_LEFT_283_TM=59.104 PRIMER_RIGHT_283_TM=58.850 PRIMER_LEFT_283_GC_PERCENT=50.000 PRIMER_RIGHT_283_GC_PERCENT=42.857 PRIMER_LEFT_283_SELF_ANY=6.00 PRIMER_RIGHT_283_SELF_ANY=3.00 PRIMER_LEFT_283_SELF_END=2.00 PRIMER_RIGHT_283_SELF_END=2.00 PRIMER_LEFT_283_END_STABILITY=5.6000 PRIMER_RIGHT_283_END_STABILITY=6.9000 PRIMER_PAIR_283_COMPL_ANY=5.00 PRIMER_PAIR_283_COMPL_END=2.00 PRIMER_PAIR_283_PRODUCT_SIZE=100 PRIMER_PAIR_284_PENALTY=5.048065 PRIMER_LEFT_284_PENALTY=1.183296 PRIMER_RIGHT_284_PENALTY=3.864768 PRIMER_LEFT_284_SEQUENCE=AGATCCATCAGGGACCAAAAC PRIMER_RIGHT_284_SEQUENCE=GCAGTTCAGGGTAGACTTCTTG PRIMER_LEFT_284=361,21 PRIMER_RIGHT_284=440,22 PRIMER_LEFT_284_TM=60.183 PRIMER_RIGHT_284_TM=58.135 PRIMER_LEFT_284_GC_PERCENT=47.619 PRIMER_RIGHT_284_GC_PERCENT=50.000 PRIMER_LEFT_284_SELF_ANY=5.00 PRIMER_RIGHT_284_SELF_ANY=4.00 PRIMER_LEFT_284_SELF_END=0.00 PRIMER_RIGHT_284_SELF_END=1.00 PRIMER_LEFT_284_END_STABILITY=7.0000 PRIMER_RIGHT_284_END_STABILITY=7.0000 PRIMER_PAIR_284_COMPL_ANY=3.00 PRIMER_PAIR_284_COMPL_END=2.00 PRIMER_PAIR_284_PRODUCT_SIZE=80 PRIMER_PAIR_285_PENALTY=5.057903 PRIMER_LEFT_285_PENALTY=2.752572 PRIMER_RIGHT_285_PENALTY=2.305331 PRIMER_LEFT_285_SEQUENCE=GATCACCAATGTGGTAGAAGCC PRIMER_RIGHT_285_SEQUENCE=TCACAAAGTGGGGATGGG PRIMER_LEFT_285=442,22 PRIMER_RIGHT_285=536,18 PRIMER_LEFT_285_TM=60.753 PRIMER_RIGHT_285_TM=60.305 PRIMER_LEFT_285_GC_PERCENT=50.000 PRIMER_RIGHT_285_GC_PERCENT=55.556 PRIMER_LEFT_285_SELF_ANY=6.00 PRIMER_RIGHT_285_SELF_ANY=3.00 PRIMER_LEFT_285_SELF_END=1.00 PRIMER_RIGHT_285_SELF_END=0.00 PRIMER_LEFT_285_END_STABILITY=9.7000 PRIMER_RIGHT_285_END_STABILITY=9.6000 PRIMER_PAIR_285_COMPL_ANY=6.00 PRIMER_PAIR_285_COMPL_END=2.00 PRIMER_PAIR_285_PRODUCT_SIZE=95 PRIMER_PAIR_286_PENALTY=5.068898 PRIMER_LEFT_286_PENALTY=2.828479 PRIMER_RIGHT_286_PENALTY=2.240419 PRIMER_LEFT_286_SEQUENCE=AACCAACCAGTGACCATCC PRIMER_RIGHT_286_SEQUENCE=ACTCACCAACTAAGCAGCGGT PRIMER_LEFT_286=464,19 PRIMER_RIGHT_286=563,21 PRIMER_LEFT_286_TM=58.172 PRIMER_RIGHT_286_TM=61.240 PRIMER_LEFT_286_GC_PERCENT=52.632 PRIMER_RIGHT_286_GC_PERCENT=52.381 PRIMER_LEFT_286_SELF_ANY=3.00 PRIMER_RIGHT_286_SELF_ANY=3.00 PRIMER_LEFT_286_SELF_END=0.00 PRIMER_RIGHT_286_SELF_END=2.00 PRIMER_LEFT_286_END_STABILITY=8.1000 PRIMER_RIGHT_286_END_STABILITY=11.1000 PRIMER_PAIR_286_COMPL_ANY=4.00 PRIMER_PAIR_286_COMPL_END=1.00 PRIMER_PAIR_286_PRODUCT_SIZE=100 PRIMER_PAIR_287_PENALTY=5.083942 PRIMER_LEFT_287_PENALTY=1.075611 PRIMER_RIGHT_287_PENALTY=4.008330 PRIMER_LEFT_287_SEQUENCE=CCAGAATGGGAAGTGGGATT PRIMER_RIGHT_287_SEQUENCE=CAGTTCAGGGTAGACTTCTTGG PRIMER_LEFT_287=340,20 PRIMER_RIGHT_287=439,22 PRIMER_LEFT_287_TM=61.076 PRIMER_RIGHT_287_TM=57.992 PRIMER_LEFT_287_GC_PERCENT=50.000 PRIMER_RIGHT_287_GC_PERCENT=50.000 PRIMER_LEFT_287_SELF_ANY=3.00 PRIMER_RIGHT_287_SELF_ANY=4.00 PRIMER_LEFT_287_SELF_END=1.00 PRIMER_RIGHT_287_SELF_END=0.00 PRIMER_LEFT_287_END_STABILITY=8.1000 PRIMER_RIGHT_287_END_STABILITY=8.5000 PRIMER_PAIR_287_COMPL_ANY=5.00 PRIMER_PAIR_287_COMPL_END=0.00 PRIMER_PAIR_287_PRODUCT_SIZE=100 PRIMER_PAIR_288_PENALTY=5.100833 PRIMER_LEFT_288_PENALTY=1.236064 PRIMER_RIGHT_288_PENALTY=3.864768 PRIMER_LEFT_288_SEQUENCE=CCATCAGGGACCAAAACCT PRIMER_RIGHT_288_SEQUENCE=TGCAGTTCAGGGTAGACTTCTT PRIMER_LEFT_288=365,19 PRIMER_RIGHT_288=441,22 PRIMER_LEFT_288_TM=59.764 PRIMER_RIGHT_288_TM=58.135 PRIMER_LEFT_288_GC_PERCENT=52.632 PRIMER_RIGHT_288_GC_PERCENT=45.455 PRIMER_LEFT_288_SELF_ANY=3.00 PRIMER_RIGHT_288_SELF_ANY=4.00 PRIMER_LEFT_288_SELF_END=0.00 PRIMER_RIGHT_288_SELF_END=2.00 PRIMER_LEFT_288_END_STABILITY=7.9000 PRIMER_RIGHT_288_END_STABILITY=7.0000 PRIMER_PAIR_288_COMPL_ANY=3.00 PRIMER_PAIR_288_COMPL_END=1.00 PRIMER_PAIR_288_PRODUCT_SIZE=77 PRIMER_PAIR_289_PENALTY=5.123933 PRIMER_LEFT_289_PENALTY=3.216208 PRIMER_RIGHT_289_PENALTY=1.907724 PRIMER_LEFT_289_SEQUENCE=GTTCTGTGGCAGACTGAACAT PRIMER_RIGHT_289_SEQUENCE=ATGCAGGTTTTGGTCCCTG PRIMER_LEFT_289=307,21 PRIMER_RIGHT_289=387,19 PRIMER_LEFT_289_TM=57.784 PRIMER_RIGHT_289_TM=60.908 PRIMER_LEFT_289_GC_PERCENT=47.619 PRIMER_RIGHT_289_GC_PERCENT=52.632 PRIMER_LEFT_289_SELF_ANY=6.00 PRIMER_RIGHT_289_SELF_ANY=4.00 PRIMER_LEFT_289_SELF_END=3.00 PRIMER_RIGHT_289_SELF_END=1.00 PRIMER_LEFT_289_END_STABILITY=6.6000 PRIMER_RIGHT_289_END_STABILITY=9.7000 PRIMER_PAIR_289_COMPL_ANY=3.00 PRIMER_PAIR_289_COMPL_END=1.00 PRIMER_PAIR_289_PRODUCT_SIZE=81 PRIMER_PAIR_290_PENALTY=5.165025 PRIMER_LEFT_290_PENALTY=1.938889 PRIMER_RIGHT_290_PENALTY=3.226137 PRIMER_LEFT_290_SEQUENCE=TTGCGAAACTCATCTTCACTG PRIMER_RIGHT_290_SEQUENCE=GGCAGCAACATGCCGTAGT PRIMER_LEFT_290=628,21 PRIMER_RIGHT_290=717,19 PRIMER_LEFT_290_TM=59.061 PRIMER_RIGHT_290_TM=62.226 PRIMER_LEFT_290_GC_PERCENT=42.857 PRIMER_RIGHT_290_GC_PERCENT=57.895 PRIMER_LEFT_290_SELF_ANY=4.00 PRIMER_RIGHT_290_SELF_ANY=4.00 PRIMER_LEFT_290_SELF_END=3.00 PRIMER_RIGHT_290_SELF_END=1.00 PRIMER_LEFT_290_END_STABILITY=6.7000 PRIMER_RIGHT_290_END_STABILITY=5.1000 PRIMER_PAIR_290_COMPL_ANY=4.00 PRIMER_PAIR_290_COMPL_END=2.00 PRIMER_PAIR_290_PRODUCT_SIZE=90 PRIMER_PAIR_291_PENALTY=5.166383 PRIMER_LEFT_291_PENALTY=1.785441 PRIMER_RIGHT_291_PENALTY=3.380942 PRIMER_LEFT_291_SEQUENCE=ACACCAGGAGAGGATGGATGT PRIMER_RIGHT_291_SEQUENCE=GCCGTAGTCATGCAAGTTGGT PRIMER_LEFT_291=607,21 PRIMER_RIGHT_291=706,21 PRIMER_LEFT_291_TM=60.785 PRIMER_RIGHT_291_TM=62.381 PRIMER_LEFT_291_GC_PERCENT=52.381 PRIMER_RIGHT_291_GC_PERCENT=52.381 PRIMER_LEFT_291_SELF_ANY=4.00 PRIMER_RIGHT_291_SELF_ANY=5.00 PRIMER_LEFT_291_SELF_END=0.00 PRIMER_RIGHT_291_SELF_END=1.00 PRIMER_LEFT_291_END_STABILITY=6.3000 PRIMER_RIGHT_291_END_STABILITY=8.2000 PRIMER_PAIR_291_COMPL_ANY=4.00 PRIMER_PAIR_291_COMPL_END=0.00 PRIMER_PAIR_291_PRODUCT_SIZE=100 PRIMER_PAIR_292_PENALTY=5.171362 PRIMER_LEFT_292_PENALTY=3.413779 PRIMER_RIGHT_292_PENALTY=1.757583 PRIMER_LEFT_292_SEQUENCE=CTGGCTGAACCCCAGATT PRIMER_RIGHT_292_SEQUENCE=ATCTGAATCCCACTTCCCAT PRIMER_LEFT_292=284,18 PRIMER_RIGHT_292=364,20 PRIMER_LEFT_292_TM=58.586 PRIMER_RIGHT_292_TM=58.242 PRIMER_LEFT_292_GC_PERCENT=55.556 PRIMER_RIGHT_292_GC_PERCENT=45.000 PRIMER_LEFT_292_SELF_ANY=4.00 PRIMER_RIGHT_292_SELF_ANY=3.00 PRIMER_LEFT_292_SELF_END=1.00 PRIMER_RIGHT_292_SELF_END=2.00 PRIMER_LEFT_292_END_STABILITY=6.6000 PRIMER_RIGHT_292_END_STABILITY=9.6000 PRIMER_PAIR_292_COMPL_ANY=5.00 PRIMER_PAIR_292_COMPL_END=1.00 PRIMER_PAIR_292_PRODUCT_SIZE=81 PRIMER_PAIR_293_PENALTY=5.206692 PRIMER_LEFT_293_PENALTY=2.847471 PRIMER_RIGHT_293_PENALTY=2.359221 PRIMER_LEFT_293_SEQUENCE=ACCCATCCCCACTTTGTG PRIMER_RIGHT_293_SEQUENCE=GTCAGGAACGAGAAGGGCA PRIMER_LEFT_293=518,18 PRIMER_RIGHT_293=592,19 PRIMER_LEFT_293_TM=59.153 PRIMER_RIGHT_293_TM=61.359 PRIMER_LEFT_293_GC_PERCENT=55.556 PRIMER_RIGHT_293_GC_PERCENT=57.895 PRIMER_LEFT_293_SELF_ANY=3.00 PRIMER_RIGHT_293_SELF_ANY=2.00 PRIMER_LEFT_293_SELF_END=3.00 PRIMER_RIGHT_293_SELF_END=0.00 PRIMER_LEFT_293_END_STABILITY=7.0000 PRIMER_RIGHT_293_END_STABILITY=11.2000 PRIMER_PAIR_293_COMPL_ANY=4.00 PRIMER_PAIR_293_COMPL_END=2.00 PRIMER_PAIR_293_PRODUCT_SIZE=75 PRIMER_PAIR_294_PENALTY=5.207392 PRIMER_LEFT_294_PENALTY=3.462244 PRIMER_RIGHT_294_PENALTY=1.745148 PRIMER_LEFT_294_SEQUENCE=CACACCGTCGCCAAAGAG PRIMER_RIGHT_294_SEQUENCE=ACTCTACCCCTCGGAACTTG PRIMER_LEFT_294=650,18 PRIMER_RIGHT_294=749,20 PRIMER_LEFT_294_TM=61.462 PRIMER_RIGHT_294_TM=58.255 PRIMER_LEFT_294_GC_PERCENT=61.111 PRIMER_RIGHT_294_GC_PERCENT=55.000 PRIMER_LEFT_294_SELF_ANY=3.00 PRIMER_RIGHT_294_SELF_ANY=3.00 PRIMER_LEFT_294_SELF_END=0.00 PRIMER_RIGHT_294_SELF_END=1.00 PRIMER_LEFT_294_END_STABILITY=6.7000 PRIMER_RIGHT_294_END_STABILITY=6.7000 PRIMER_PAIR_294_COMPL_ANY=4.00 PRIMER_PAIR_294_COMPL_END=0.00 PRIMER_PAIR_294_PRODUCT_SIZE=100 PRIMER_PAIR_295_PENALTY=5.222637 PRIMER_LEFT_295_PENALTY=1.707711 PRIMER_RIGHT_295_PENALTY=3.514925 PRIMER_LEFT_295_SEQUENCE=CATGAATGTCCAGAATGGGAA PRIMER_RIGHT_295_SEQUENCE=GCAGGATGCCTTCCTTGGT PRIMER_LEFT_295=331,21 PRIMER_RIGHT_295=410,19 PRIMER_LEFT_295_TM=60.708 PRIMER_RIGHT_295_TM=62.515 PRIMER_LEFT_295_GC_PERCENT=42.857 PRIMER_RIGHT_295_GC_PERCENT=57.895 PRIMER_LEFT_295_SELF_ANY=4.00 PRIMER_RIGHT_295_SELF_ANY=7.00 PRIMER_LEFT_295_SELF_END=1.00 PRIMER_RIGHT_295_SELF_END=1.00 PRIMER_LEFT_295_END_STABILITY=9.7000 PRIMER_RIGHT_295_END_STABILITY=8.2000 PRIMER_PAIR_295_COMPL_ANY=4.00 PRIMER_PAIR_295_COMPL_END=1.00 PRIMER_PAIR_295_PRODUCT_SIZE=80 PRIMER_PAIR_296_PENALTY=5.225323 PRIMER_LEFT_296_PENALTY=2.486287 PRIMER_RIGHT_296_PENALTY=2.739036 PRIMER_LEFT_296_SEQUENCE=TGCCCTTCTCGTTCCTGA PRIMER_RIGHT_296_SEQUENCE=GTGTGCCAGTGAAGATGAGTTT PRIMER_LEFT_296=574,18 PRIMER_RIGHT_296=654,22 PRIMER_LEFT_296_TM=60.486 PRIMER_RIGHT_296_TM=59.261 PRIMER_LEFT_296_GC_PERCENT=55.556 PRIMER_RIGHT_296_GC_PERCENT=45.455 PRIMER_LEFT_296_SELF_ANY=2.00 PRIMER_RIGHT_296_SELF_ANY=3.00 PRIMER_LEFT_296_SELF_END=2.00 PRIMER_RIGHT_296_SELF_END=1.00 PRIMER_LEFT_296_END_STABILITY=8.2000 PRIMER_RIGHT_296_END_STABILITY=6.7000 PRIMER_PAIR_296_COMPL_ANY=4.00 PRIMER_PAIR_296_COMPL_END=1.00 PRIMER_PAIR_296_PRODUCT_SIZE=81 PRIMER_PAIR_297_PENALTY=5.246680 PRIMER_LEFT_297_PENALTY=1.357294 PRIMER_RIGHT_297_PENALTY=3.889386 PRIMER_LEFT_297_SEQUENCE=AATGGGAAGTGGGATTCAGAT PRIMER_RIGHT_297_SEQUENCE=CTGCAGTTCAGGGTAGACTTCTT PRIMER_LEFT_297=344,21 PRIMER_RIGHT_297=442,23 PRIMER_LEFT_297_TM=59.643 PRIMER_RIGHT_297_TM=59.111 PRIMER_LEFT_297_GC_PERCENT=42.857 PRIMER_RIGHT_297_GC_PERCENT=47.826 PRIMER_LEFT_297_SELF_ANY=3.00 PRIMER_RIGHT_297_SELF_ANY=6.00 PRIMER_LEFT_297_SELF_END=2.00 PRIMER_RIGHT_297_SELF_END=2.00 PRIMER_LEFT_297_END_STABILITY=6.6000 PRIMER_RIGHT_297_END_STABILITY=7.0000 PRIMER_PAIR_297_COMPL_ANY=5.00 PRIMER_PAIR_297_COMPL_END=1.00 PRIMER_PAIR_297_PRODUCT_SIZE=99 PRIMER_PAIR_298_PENALTY=5.253631 PRIMER_LEFT_298_PENALTY=2.487598 PRIMER_RIGHT_298_PENALTY=2.766033 PRIMER_LEFT_298_SEQUENCE=GTGATTCCCTACCGCTGCTTA PRIMER_RIGHT_298_SEQUENCE=ACATCCATCCTCTCCTGGTGTA PRIMER_LEFT_298=533,21 PRIMER_RIGHT_298=627,22 PRIMER_LEFT_298_TM=61.488 PRIMER_RIGHT_298_TM=60.766 PRIMER_LEFT_298_GC_PERCENT=52.381 PRIMER_RIGHT_298_GC_PERCENT=50.000 PRIMER_LEFT_298_SELF_ANY=3.00 PRIMER_RIGHT_298_SELF_ANY=4.00 PRIMER_LEFT_298_SELF_END=2.00 PRIMER_RIGHT_298_SELF_END=2.00 PRIMER_LEFT_298_END_STABILITY=7.5000 PRIMER_RIGHT_298_END_STABILITY=5.4000 PRIMER_PAIR_298_COMPL_ANY=3.00 PRIMER_PAIR_298_COMPL_END=2.00 PRIMER_PAIR_298_PRODUCT_SIZE=95 PRIMER_PAIR_299_PENALTY=5.287977 PRIMER_LEFT_299_PENALTY=1.818509 PRIMER_RIGHT_299_PENALTY=3.469469 PRIMER_LEFT_299_SEQUENCE=AGAGGATGGATGTTTGCGA PRIMER_RIGHT_299_SEQUENCE=CCGTAGTCATGCAAGTTGGTACT PRIMER_LEFT_299=615,19 PRIMER_RIGHT_299=705,23 PRIMER_LEFT_299_TM=59.181 PRIMER_RIGHT_299_TM=60.469 PRIMER_LEFT_299_GC_PERCENT=47.368 PRIMER_RIGHT_299_GC_PERCENT=47.826 PRIMER_LEFT_299_SELF_ANY=2.00 PRIMER_RIGHT_299_SELF_ANY=5.00 PRIMER_LEFT_299_SELF_END=2.00 PRIMER_RIGHT_299_SELF_END=3.00 PRIMER_LEFT_299_END_STABILITY=10.2000 PRIMER_RIGHT_299_END_STABILITY=5.1000 PRIMER_PAIR_299_COMPL_ANY=4.00 PRIMER_PAIR_299_COMPL_END=1.00 PRIMER_PAIR_299_PRODUCT_SIZE=91 PRIMER_PAIR_300_PENALTY=5.293310 PRIMER_LEFT_300_PENALTY=2.384789 PRIMER_RIGHT_300_PENALTY=2.908522 PRIMER_LEFT_300_SEQUENCE=CTCGTTCCTGACAAGTGCAAA PRIMER_RIGHT_300_SEQUENCE=GGTGTGCCAGTGAAGATGA PRIMER_LEFT_300=581,21 PRIMER_RIGHT_300=655,19 PRIMER_LEFT_300_TM=61.385 PRIMER_RIGHT_300_TM=58.091 PRIMER_LEFT_300_GC_PERCENT=47.619 PRIMER_RIGHT_300_GC_PERCENT=52.632 PRIMER_LEFT_300_SELF_ANY=4.00 PRIMER_RIGHT_300_SELF_ANY=3.00 PRIMER_LEFT_300_SELF_END=0.00 PRIMER_RIGHT_300_SELF_END=1.00 PRIMER_LEFT_300_END_STABILITY=8.8000 PRIMER_RIGHT_300_END_STABILITY=6.6000 PRIMER_PAIR_300_COMPL_ANY=5.00 PRIMER_PAIR_300_COMPL_END=1.00 PRIMER_PAIR_300_PRODUCT_SIZE=75 PRIMER_PAIR_301_PENALTY=5.306705 PRIMER_LEFT_301_PENALTY=3.269366 PRIMER_RIGHT_301_PENALTY=2.037339 PRIMER_LEFT_301_SEQUENCE=CATCCAGAACTGGTGCAAG PRIMER_RIGHT_301_SEQUENCE=GGCATCACTTACAAACTCACCA PRIMER_LEFT_301=478,19 PRIMER_RIGHT_301=577,22 PRIMER_LEFT_301_TM=57.731 PRIMER_RIGHT_301_TM=60.037 PRIMER_LEFT_301_GC_PERCENT=52.632 PRIMER_RIGHT_301_GC_PERCENT=45.455 PRIMER_LEFT_301_SELF_ANY=6.00 PRIMER_RIGHT_301_SELF_ANY=2.00 PRIMER_LEFT_301_SELF_END=2.00 PRIMER_RIGHT_301_SELF_END=0.00 PRIMER_LEFT_301_END_STABILITY=8.5000 PRIMER_RIGHT_301_END_STABILITY=8.2000 PRIMER_PAIR_301_COMPL_ANY=5.00 PRIMER_PAIR_301_COMPL_END=3.00 PRIMER_PAIR_301_PRODUCT_SIZE=100 PRIMER_PAIR_302_PENALTY=5.324262 PRIMER_LEFT_302_PENALTY=3.279220 PRIMER_RIGHT_302_PENALTY=2.045042 PRIMER_LEFT_302_SEQUENCE=ACCCCAGATTGCCATGTT PRIMER_RIGHT_302_SEQUENCE=CTGATGGATCTGAATCCCACT PRIMER_LEFT_302=292,18 PRIMER_RIGHT_302=371,21 PRIMER_LEFT_302_TM=58.721 PRIMER_RIGHT_302_TM=58.955 PRIMER_LEFT_302_GC_PERCENT=50.000 PRIMER_RIGHT_302_GC_PERCENT=47.619 PRIMER_LEFT_302_SELF_ANY=4.00 PRIMER_RIGHT_302_SELF_ANY=5.00 PRIMER_LEFT_302_SELF_END=2.00 PRIMER_RIGHT_302_SELF_END=1.00 PRIMER_LEFT_302_END_STABILITY=6.6000 PRIMER_RIGHT_302_END_STABILITY=7.9000 PRIMER_PAIR_302_COMPL_ANY=5.00 PRIMER_PAIR_302_COMPL_END=0.00 PRIMER_PAIR_302_PRODUCT_SIZE=80 PRIMER_PAIR_303_PENALTY=5.329358 PRIMER_LEFT_303_PENALTY=3.291759 PRIMER_RIGHT_303_PENALTY=2.037599 PRIMER_LEFT_303_SEQUENCE=CCATGTTCTGTGGCAGACTGA PRIMER_RIGHT_303_SEQUENCE=CCTTGGTATCAATGCAGGTTT PRIMER_LEFT_303=303,21 PRIMER_RIGHT_303=398,21 PRIMER_LEFT_303_TM=62.292 PRIMER_RIGHT_303_TM=58.962 PRIMER_LEFT_303_GC_PERCENT=52.381 PRIMER_RIGHT_303_GC_PERCENT=42.857 PRIMER_LEFT_303_SELF_ANY=5.00 PRIMER_RIGHT_303_SELF_ANY=6.00 PRIMER_LEFT_303_SELF_END=3.00 PRIMER_RIGHT_303_SELF_END=1.00 PRIMER_LEFT_303_END_STABILITY=6.4000 PRIMER_RIGHT_303_END_STABILITY=8.2000 PRIMER_PAIR_303_COMPL_ANY=3.00 PRIMER_PAIR_303_COMPL_END=1.00 PRIMER_PAIR_303_PRODUCT_SIZE=96 PRIMER_PAIR_304_PENALTY=5.352938 PRIMER_LEFT_304_PENALTY=3.571483 PRIMER_RIGHT_304_PENALTY=1.781455 PRIMER_LEFT_304_SEQUENCE=CCTGCAGTATTGCCAAGAA PRIMER_RIGHT_304_SEQUENCE=ATGGTCACTGGTTGGTTGG PRIMER_LEFT_304=406,19 PRIMER_RIGHT_304=480,19 PRIMER_LEFT_304_TM=57.429 PRIMER_RIGHT_304_TM=59.219 PRIMER_LEFT_304_GC_PERCENT=47.368 PRIMER_RIGHT_304_GC_PERCENT=52.632 PRIMER_LEFT_304_SELF_ANY=6.00 PRIMER_RIGHT_304_SELF_ANY=3.00 PRIMER_LEFT_304_SELF_END=3.00 PRIMER_RIGHT_304_SELF_END=0.00 PRIMER_LEFT_304_END_STABILITY=7.0000 PRIMER_RIGHT_304_END_STABILITY=8.2000 PRIMER_PAIR_304_COMPL_ANY=4.00 PRIMER_PAIR_304_COMPL_END=3.00 PRIMER_PAIR_304_PRODUCT_SIZE=75 PRIMER_PAIR_305_PENALTY=5.354170 PRIMER_LEFT_305_PENALTY=1.278124 PRIMER_RIGHT_305_PENALTY=4.076046 PRIMER_LEFT_305_SEQUENCE=CCAGAATGGGAAGTGGGAT PRIMER_RIGHT_305_SEQUENCE=CAGGGTAGACTTCTTGGCAATACT PRIMER_LEFT_305=340,19 PRIMER_RIGHT_305=434,24 PRIMER_LEFT_305_TM=59.722 PRIMER_RIGHT_305_TM=60.076 PRIMER_LEFT_305_GC_PERCENT=52.632 PRIMER_RIGHT_305_GC_PERCENT=45.833 PRIMER_LEFT_305_SELF_ANY=3.00 PRIMER_RIGHT_305_SELF_ANY=5.00 PRIMER_LEFT_305_SELF_END=2.00 PRIMER_RIGHT_305_SELF_END=2.00 PRIMER_LEFT_305_END_STABILITY=9.3000 PRIMER_RIGHT_305_END_STABILITY=5.3000 PRIMER_PAIR_305_COMPL_ANY=5.00 PRIMER_PAIR_305_COMPL_END=1.00 PRIMER_PAIR_305_PRODUCT_SIZE=95 PRIMER_PAIR_306_PENALTY=5.364772 PRIMER_LEFT_306_PENALTY=1.798843 PRIMER_RIGHT_306_PENALTY=3.565929 PRIMER_LEFT_306_SEQUENCE=ACATGAATGTCCAGAATGGG PRIMER_RIGHT_306_SEQUENCE=GCAGGATGCCTTCCTTGGTAT PRIMER_LEFT_306=330,20 PRIMER_RIGHT_306=410,21 PRIMER_LEFT_306_TM=58.201 PRIMER_RIGHT_306_TM=62.566 PRIMER_LEFT_306_GC_PERCENT=45.000 PRIMER_RIGHT_306_GC_PERCENT=52.381 PRIMER_LEFT_306_SELF_ANY=6.00 PRIMER_RIGHT_306_SELF_ANY=7.00 PRIMER_LEFT_306_SELF_END=1.00 PRIMER_RIGHT_306_SELF_END=2.00 PRIMER_LEFT_306_END_STABILITY=9.6000 PRIMER_RIGHT_306_END_STABILITY=6.8000 PRIMER_PAIR_306_COMPL_ANY=3.00 PRIMER_PAIR_306_COMPL_END=2.00 PRIMER_PAIR_306_PRODUCT_SIZE=81 PRIMER_PAIR_307_PENALTY=5.380369 PRIMER_LEFT_307_PENALTY=2.623229 PRIMER_RIGHT_307_PENALTY=2.757140 PRIMER_LEFT_307_SEQUENCE=TTCCCTACCGCTGCTTAGTTG PRIMER_RIGHT_307_SEQUENCE=GTTTCGCAAACATCCATCCTC PRIMER_LEFT_307=537,21 PRIMER_RIGHT_307=636,21 PRIMER_LEFT_307_TM=61.623 PRIMER_RIGHT_307_TM=61.757 PRIMER_LEFT_307_GC_PERCENT=52.381 PRIMER_RIGHT_307_GC_PERCENT=47.619 PRIMER_LEFT_307_SELF_ANY=3.00 PRIMER_RIGHT_307_SELF_ANY=6.00 PRIMER_LEFT_307_SELF_END=1.00 PRIMER_RIGHT_307_SELF_END=0.00 PRIMER_LEFT_307_END_STABILITY=6.7000 PRIMER_RIGHT_307_END_STABILITY=7.9000 PRIMER_PAIR_307_COMPL_ANY=3.00 PRIMER_PAIR_307_COMPL_END=1.00 PRIMER_PAIR_307_PRODUCT_SIZE=100 PRIMER_PAIR_308_PENALTY=5.384900 PRIMER_LEFT_308_PENALTY=1.818509 PRIMER_RIGHT_308_PENALTY=3.566391 PRIMER_LEFT_308_SEQUENCE=GAGAGGATGGATGTTTGCG PRIMER_RIGHT_308_SEQUENCE=CATGCAAGTTGGTACTCTTCTCA PRIMER_LEFT_308=614,19 PRIMER_RIGHT_308=698,23 PRIMER_LEFT_308_TM=59.181 PRIMER_RIGHT_308_TM=59.434 PRIMER_LEFT_308_GC_PERCENT=52.632 PRIMER_RIGHT_308_GC_PERCENT=43.478 PRIMER_LEFT_308_SELF_ANY=2.00 PRIMER_RIGHT_308_SELF_ANY=5.00 PRIMER_LEFT_308_SELF_END=2.00 PRIMER_RIGHT_308_SELF_END=1.00 PRIMER_LEFT_308_END_STABILITY=10.5000 PRIMER_RIGHT_308_END_STABILITY=6.7000 PRIMER_PAIR_308_COMPL_ANY=5.00 PRIMER_PAIR_308_COMPL_END=2.00 PRIMER_PAIR_308_PRODUCT_SIZE=85 PRIMER_PAIR_309_PENALTY=5.388009 PRIMER_LEFT_309_PENALTY=2.901722 PRIMER_RIGHT_309_PENALTY=2.486287 PRIMER_LEFT_309_SEQUENCE=GCAAGACCCATCCCCACT PRIMER_RIGHT_309_SEQUENCE=TCAGGAACGAGAAGGGCA PRIMER_LEFT_309=513,18 PRIMER_RIGHT_309=591,18 PRIMER_LEFT_309_TM=60.902 PRIMER_RIGHT_309_TM=60.486 PRIMER_LEFT_309_GC_PERCENT=61.111 PRIMER_RIGHT_309_GC_PERCENT=55.556 PRIMER_LEFT_309_SELF_ANY=2.00 PRIMER_RIGHT_309_SELF_ANY=2.00 PRIMER_LEFT_309_SELF_END=1.00 PRIMER_RIGHT_309_SELF_END=0.00 PRIMER_LEFT_309_END_STABILITY=7.9000 PRIMER_RIGHT_309_END_STABILITY=11.2000 PRIMER_PAIR_309_COMPL_ANY=4.00 PRIMER_PAIR_309_COMPL_END=2.00 PRIMER_PAIR_309_PRODUCT_SIZE=79 PRIMER_PAIR_310_PENALTY=5.390157 PRIMER_LEFT_310_PENALTY=2.601481 PRIMER_RIGHT_310_PENALTY=2.788676 PRIMER_LEFT_310_SEQUENCE=TGATTCCCTACCGCTGCTTAG PRIMER_RIGHT_310_SEQUENCE=CAAACATCCATCCTCTCCTGG PRIMER_LEFT_310=534,21 PRIMER_RIGHT_310=630,21 PRIMER_LEFT_310_TM=61.601 PRIMER_RIGHT_310_TM=61.789 PRIMER_LEFT_310_GC_PERCENT=52.381 PRIMER_RIGHT_310_GC_PERCENT=52.381 PRIMER_LEFT_310_SELF_ANY=3.00 PRIMER_RIGHT_310_SELF_ANY=3.00 PRIMER_LEFT_310_SELF_END=3.00 PRIMER_RIGHT_310_SELF_END=3.00 PRIMER_LEFT_310_END_STABILITY=6.0000 PRIMER_RIGHT_310_END_STABILITY=9.7000 PRIMER_PAIR_310_COMPL_ANY=3.00 PRIMER_PAIR_310_COMPL_END=0.00 PRIMER_PAIR_310_PRODUCT_SIZE=97 PRIMER_PAIR_311_PENALTY=5.419477 PRIMER_LEFT_311_PENALTY=2.933397 PRIMER_RIGHT_311_PENALTY=2.486079 PRIMER_LEFT_311_SEQUENCE=AACTGCAGATCACCAATGTG PRIMER_RIGHT_311_SEQUENCE=TGGGTCTTGCACTGCTTG PRIMER_LEFT_311=435,20 PRIMER_RIGHT_311=522,18 PRIMER_LEFT_311_TM=57.067 PRIMER_RIGHT_311_TM=59.514 PRIMER_LEFT_311_GC_PERCENT=45.000 PRIMER_RIGHT_311_GC_PERCENT=55.556 PRIMER_LEFT_311_SELF_ANY=6.00 PRIMER_RIGHT_311_SELF_ANY=5.00 PRIMER_LEFT_311_SELF_END=3.00 PRIMER_RIGHT_311_SELF_END=3.00 PRIMER_LEFT_311_END_STABILITY=6.6000 PRIMER_RIGHT_311_END_STABILITY=8.5000 PRIMER_PAIR_311_COMPL_ANY=4.00 PRIMER_PAIR_311_COMPL_END=1.00 PRIMER_PAIR_311_PRODUCT_SIZE=88 PRIMER_PAIR_312_PENALTY=5.444251 PRIMER_LEFT_312_PENALTY=3.937317 PRIMER_RIGHT_312_PENALTY=1.506934 PRIMER_LEFT_312_SEQUENCE=CGTCGCCAAAGAGACATGC PRIMER_RIGHT_312_SEQUENCE=ACAAACTCTACCCCTCGGAAC PRIMER_LEFT_312=655,19 PRIMER_RIGHT_312=753,21 PRIMER_LEFT_312_TM=62.937 PRIMER_RIGHT_312_TM=59.493 PRIMER_LEFT_312_GC_PERCENT=57.895 PRIMER_RIGHT_312_GC_PERCENT=52.381 PRIMER_LEFT_312_SELF_ANY=4.00 PRIMER_RIGHT_312_SELF_ANY=3.00 PRIMER_LEFT_312_SELF_END=2.00 PRIMER_RIGHT_312_SELF_END=0.00 PRIMER_LEFT_312_END_STABILITY=8.4000 PRIMER_RIGHT_312_END_STABILITY=7.9000 PRIMER_PAIR_312_COMPL_ANY=4.00 PRIMER_PAIR_312_COMPL_END=0.00 PRIMER_PAIR_312_PRODUCT_SIZE=99 PRIMER_PAIR_313_PENALTY=5.459430 PRIMER_LEFT_313_PENALTY=2.813295 PRIMER_RIGHT_313_PENALTY=2.646135 PRIMER_LEFT_313_SEQUENCE=CCATCCCCACTTTGTGATT PRIMER_RIGHT_313_SEQUENCE=TTGTCAGGAACGAGAAGGGC PRIMER_LEFT_313=520,19 PRIMER_RIGHT_313=594,20 PRIMER_LEFT_313_TM=58.187 PRIMER_RIGHT_313_TM=62.646 PRIMER_LEFT_313_GC_PERCENT=47.368 PRIMER_RIGHT_313_GC_PERCENT=55.000 PRIMER_LEFT_313_SELF_ANY=4.00 PRIMER_RIGHT_313_SELF_ANY=3.00 PRIMER_LEFT_313_SELF_END=2.00 PRIMER_RIGHT_313_SELF_END=2.00 PRIMER_LEFT_313_END_STABILITY=6.9000 PRIMER_RIGHT_313_END_STABILITY=10.9000 PRIMER_PAIR_313_COMPL_ANY=3.00 PRIMER_PAIR_313_COMPL_END=0.00 PRIMER_PAIR_313_PRODUCT_SIZE=75 PRIMER_PAIR_314_PENALTY=5.467190 PRIMER_LEFT_314_PENALTY=2.710050 PRIMER_RIGHT_314_PENALTY=2.757140 PRIMER_LEFT_314_SEQUENCE=CCGCTGCTTAGTTGGTGAGTT PRIMER_RIGHT_314_SEQUENCE=GAGTTTCGCAAACATCCATCC PRIMER_LEFT_314=544,21 PRIMER_RIGHT_314=638,21 PRIMER_LEFT_314_TM=61.710 PRIMER_RIGHT_314_TM=61.757 PRIMER_LEFT_314_GC_PERCENT=52.381 PRIMER_RIGHT_314_GC_PERCENT=47.619 PRIMER_LEFT_314_SELF_ANY=3.00 PRIMER_RIGHT_314_SELF_ANY=6.00 PRIMER_LEFT_314_SELF_END=0.00 PRIMER_RIGHT_314_SELF_END=0.00 PRIMER_LEFT_314_END_STABILITY=6.4000 PRIMER_RIGHT_314_END_STABILITY=8.1000 PRIMER_PAIR_314_COMPL_ANY=4.00 PRIMER_PAIR_314_COMPL_END=2.00 PRIMER_PAIR_314_PRODUCT_SIZE=95 PRIMER_PAIR_315_PENALTY=5.473658 PRIMER_LEFT_315_PENALTY=2.640342 PRIMER_RIGHT_315_PENALTY=2.833316 PRIMER_LEFT_315_SEQUENCE=CCACTTTGTGATTCCCTACCG PRIMER_RIGHT_315_SEQUENCE=CCATCCTCTCCTGGTGTAAG PRIMER_LEFT_315=526,21 PRIMER_RIGHT_315=623,20 PRIMER_LEFT_315_TM=61.640 PRIMER_RIGHT_315_TM=57.167 PRIMER_LEFT_315_GC_PERCENT=52.381 PRIMER_RIGHT_315_GC_PERCENT=55.000 PRIMER_LEFT_315_SELF_ANY=3.00 PRIMER_RIGHT_315_SELF_ANY=3.00 PRIMER_LEFT_315_SELF_END=2.00 PRIMER_RIGHT_315_SELF_END=1.00 PRIMER_LEFT_315_END_STABILITY=8.9000 PRIMER_RIGHT_315_END_STABILITY=5.7000 PRIMER_PAIR_315_COMPL_ANY=3.00 PRIMER_PAIR_315_COMPL_END=1.00 PRIMER_PAIR_315_PRODUCT_SIZE=98 PRIMER_PAIR_316_PENALTY=5.481415 PRIMER_LEFT_316_PENALTY=3.352274 PRIMER_RIGHT_316_PENALTY=2.129141 PRIMER_LEFT_316_SEQUENCE=CTGCAGTATTGCCAAGAAGTC PRIMER_RIGHT_316_SEQUENCE=TGCACCAGTTCTGGATGGTC PRIMER_LEFT_316=407,21 PRIMER_RIGHT_316=494,20 PRIMER_LEFT_316_TM=57.648 PRIMER_RIGHT_316_TM=62.129 PRIMER_LEFT_316_GC_PERCENT=47.619 PRIMER_RIGHT_316_GC_PERCENT=55.000 PRIMER_LEFT_316_SELF_ANY=6.00 PRIMER_RIGHT_316_SELF_ANY=6.00 PRIMER_LEFT_316_SELF_END=2.00 PRIMER_RIGHT_316_SELF_END=3.00 PRIMER_LEFT_316_END_STABILITY=6.4000 PRIMER_RIGHT_316_END_STABILITY=7.9000 PRIMER_PAIR_316_COMPL_ANY=4.00 PRIMER_PAIR_316_COMPL_END=1.00 PRIMER_PAIR_316_PRODUCT_SIZE=88 PRIMER_PAIR_317_PENALTY=5.496047 PRIMER_LEFT_317_PENALTY=3.377310 PRIMER_RIGHT_317_PENALTY=2.118737 PRIMER_LEFT_317_SEQUENCE=CACTGATGGTAATGCTGGC PRIMER_RIGHT_317_SEQUENCE=CCACTTCCCATTCTGGACATT PRIMER_LEFT_317=262,19 PRIMER_RIGHT_317=355,21 PRIMER_LEFT_317_TM=57.623 PRIMER_RIGHT_317_TM=61.119 PRIMER_LEFT_317_GC_PERCENT=52.632 PRIMER_RIGHT_317_GC_PERCENT=47.619 PRIMER_LEFT_317_SELF_ANY=3.00 PRIMER_RIGHT_317_SELF_ANY=3.00 PRIMER_LEFT_317_SELF_END=2.00 PRIMER_RIGHT_317_SELF_END=1.00 PRIMER_LEFT_317_END_STABILITY=9.7000 PRIMER_RIGHT_317_END_STABILITY=6.6000 PRIMER_PAIR_317_COMPL_ANY=5.00 PRIMER_PAIR_317_COMPL_END=1.00 PRIMER_PAIR_317_PRODUCT_SIZE=94 PRIMER_PAIR_318_PENALTY=5.505184 PRIMER_LEFT_318_PENALTY=1.440351 PRIMER_RIGHT_318_PENALTY=4.064833 PRIMER_LEFT_318_SEQUENCE=TCAGATCCATCAGGGACCAA PRIMER_RIGHT_318_SEQUENCE=ATCTGCAGTTCAGGGTAGACTTC PRIMER_LEFT_318=359,20 PRIMER_RIGHT_318=444,23 PRIMER_LEFT_318_TM=61.440 PRIMER_RIGHT_318_TM=58.935 PRIMER_LEFT_318_GC_PERCENT=50.000 PRIMER_RIGHT_318_GC_PERCENT=47.826 PRIMER_LEFT_318_SELF_ANY=5.00 PRIMER_RIGHT_318_SELF_ANY=6.00 PRIMER_LEFT_318_SELF_END=1.00 PRIMER_RIGHT_318_SELF_END=3.00 PRIMER_LEFT_318_END_STABILITY=8.2000 PRIMER_RIGHT_318_END_STABILITY=6.4000 PRIMER_PAIR_318_COMPL_ANY=5.00 PRIMER_PAIR_318_COMPL_END=1.00 PRIMER_PAIR_318_PRODUCT_SIZE=86 PRIMER_PAIR_319_PENALTY=5.505184 PRIMER_LEFT_319_PENALTY=1.440351 PRIMER_RIGHT_319_PENALTY=4.064833 PRIMER_LEFT_319_SEQUENCE=TTCAGATCCATCAGGGACCA PRIMER_RIGHT_319_SEQUENCE=GATCTGCAGTTCAGGGTAGACTT PRIMER_LEFT_319=358,20 PRIMER_RIGHT_319=445,23 PRIMER_LEFT_319_TM=61.440 PRIMER_RIGHT_319_TM=58.935 PRIMER_LEFT_319_GC_PERCENT=50.000 PRIMER_RIGHT_319_GC_PERCENT=47.826 PRIMER_LEFT_319_SELF_ANY=5.00 PRIMER_RIGHT_319_SELF_ANY=8.00 PRIMER_LEFT_319_SELF_END=3.00 PRIMER_RIGHT_319_SELF_END=1.00 PRIMER_LEFT_319_END_STABILITY=7.9000 PRIMER_RIGHT_319_END_STABILITY=6.4000 PRIMER_PAIR_319_COMPL_ANY=6.00 PRIMER_PAIR_319_COMPL_END=1.00 PRIMER_PAIR_319_PRODUCT_SIZE=88 PRIMER_PAIR_320_PENALTY=5.506140 PRIMER_LEFT_320_PENALTY=3.449712 PRIMER_RIGHT_320_PENALTY=2.056428 PRIMER_LEFT_320_SEQUENCE=TCTGTGGCAGACTGAACATGC PRIMER_RIGHT_320_SEQUENCE=GGTATCAATGCAGGTTTTGG PRIMER_LEFT_320=309,21 PRIMER_RIGHT_320=394,20 PRIMER_LEFT_320_TM=62.450 PRIMER_RIGHT_320_TM=57.944 PRIMER_LEFT_320_GC_PERCENT=52.381 PRIMER_RIGHT_320_GC_PERCENT=45.000 PRIMER_LEFT_320_SELF_ANY=5.00 PRIMER_RIGHT_320_SELF_ANY=4.00 PRIMER_LEFT_320_SELF_END=2.00 PRIMER_RIGHT_320_SELF_END=0.00 PRIMER_LEFT_320_END_STABILITY=8.4000 PRIMER_RIGHT_320_END_STABILITY=8.8000 PRIMER_PAIR_320_COMPL_ANY=5.00 PRIMER_PAIR_320_COMPL_END=3.00 PRIMER_PAIR_320_PRODUCT_SIZE=86 PRIMER_PAIR_321_PENALTY=5.507375 PRIMER_LEFT_321_PENALTY=2.785923 PRIMER_RIGHT_321_PENALTY=2.721451 PRIMER_LEFT_321_SEQUENCE=ATGCACATGAATGTCCAGAATG PRIMER_RIGHT_321_SEQUENCE=TTCCTTGGTATCAATGCAGGTT PRIMER_LEFT_321=326,22 PRIMER_RIGHT_321=400,22 PRIMER_LEFT_321_TM=60.786 PRIMER_RIGHT_321_TM=60.721 PRIMER_LEFT_321_GC_PERCENT=40.909 PRIMER_RIGHT_321_GC_PERCENT=40.909 PRIMER_LEFT_321_SELF_ANY=8.00 PRIMER_RIGHT_321_SELF_ANY=6.00 PRIMER_LEFT_321_SELF_END=1.00 PRIMER_RIGHT_321_SELF_END=0.00 PRIMER_LEFT_321_END_STABILITY=6.9000 PRIMER_RIGHT_321_END_STABILITY=7.9000 PRIMER_PAIR_321_COMPL_ANY=4.00 PRIMER_PAIR_321_COMPL_END=1.00 PRIMER_PAIR_321_PRODUCT_SIZE=75 PRIMER_PAIR_322_PENALTY=5.513615 PRIMER_LEFT_322_PENALTY=1.928663 PRIMER_RIGHT_322_PENALTY=3.584952 PRIMER_LEFT_322_SEQUENCE=CAGGAGAGGATGGATGTTTG PRIMER_RIGHT_322_SEQUENCE=TGGTACTCTTCTCACTGCATGTC PRIMER_LEFT_322=611,20 PRIMER_RIGHT_322=689,23 PRIMER_LEFT_322_TM=58.071 PRIMER_RIGHT_322_TM=59.415 PRIMER_LEFT_322_GC_PERCENT=50.000 PRIMER_RIGHT_322_GC_PERCENT=47.826 PRIMER_LEFT_322_SELF_ANY=2.00 PRIMER_RIGHT_322_SELF_ANY=4.00 PRIMER_LEFT_322_SELF_END=0.00 PRIMER_RIGHT_322_SELF_END=2.00 PRIMER_LEFT_322_END_STABILITY=7.0000 PRIMER_RIGHT_322_END_STABILITY=6.3000 PRIMER_PAIR_322_COMPL_ANY=6.00 PRIMER_PAIR_322_COMPL_END=1.00 PRIMER_PAIR_322_PRODUCT_SIZE=79 PRIMER_PAIR_323_PENALTY=5.532363 PRIMER_LEFT_323_PENALTY=2.652125 PRIMER_RIGHT_323_PENALTY=2.880239 PRIMER_LEFT_323_SEQUENCE=TGATTCCCTACCGCTGCT PRIMER_RIGHT_323_SEQUENCE=CGCAAACATCCATCCTCTCC PRIMER_LEFT_323=534,18 PRIMER_RIGHT_323=632,20 PRIMER_LEFT_323_TM=59.348 PRIMER_RIGHT_323_TM=62.880 PRIMER_LEFT_323_GC_PERCENT=55.556 PRIMER_RIGHT_323_GC_PERCENT=55.000 PRIMER_LEFT_323_SELF_ANY=3.00 PRIMER_RIGHT_323_SELF_ANY=2.00 PRIMER_LEFT_323_SELF_END=1.00 PRIMER_RIGHT_323_SELF_END=0.00 PRIMER_LEFT_323_END_STABILITY=8.2000 PRIMER_RIGHT_323_END_STABILITY=7.9000 PRIMER_PAIR_323_COMPL_ANY=3.00 PRIMER_PAIR_323_COMPL_END=0.00 PRIMER_PAIR_323_PRODUCT_SIZE=99 PRIMER_PAIR_324_PENALTY=5.533388 PRIMER_LEFT_324_PENALTY=2.901722 PRIMER_RIGHT_324_PENALTY=2.631665 PRIMER_LEFT_324_SEQUENCE=AGTGCAAGACCCATCCCC PRIMER_RIGHT_324_SEQUENCE=TCAGGAACGAGAAGGGCATC PRIMER_LEFT_324=510,18 PRIMER_RIGHT_324=591,20 PRIMER_LEFT_324_TM=60.902 PRIMER_RIGHT_324_TM=62.632 PRIMER_LEFT_324_GC_PERCENT=61.111 PRIMER_RIGHT_324_GC_PERCENT=55.000 PRIMER_LEFT_324_SELF_ANY=4.00 PRIMER_RIGHT_324_SELF_ANY=2.00 PRIMER_LEFT_324_SELF_END=0.00 PRIMER_RIGHT_324_SELF_END=2.00 PRIMER_LEFT_324_END_STABILITY=10.9000 PRIMER_RIGHT_324_END_STABILITY=8.1000 PRIMER_PAIR_324_COMPL_ANY=4.00 PRIMER_PAIR_324_COMPL_END=3.00 PRIMER_PAIR_324_PRODUCT_SIZE=82 PRIMER_PAIR_325_PENALTY=5.541878 PRIMER_LEFT_325_PENALTY=3.081950 PRIMER_RIGHT_325_PENALTY=2.459929 PRIMER_LEFT_325_SEQUENCE=CAACCAGTGACCATCCAGA PRIMER_RIGHT_325_SEQUENCE=CAACTAAGCAGCGGTAGGG PRIMER_LEFT_325=467,19 PRIMER_RIGHT_325=557,19 PRIMER_LEFT_325_TM=57.918 PRIMER_RIGHT_325_TM=58.540 PRIMER_LEFT_325_GC_PERCENT=52.632 PRIMER_RIGHT_325_GC_PERCENT=57.895 PRIMER_LEFT_325_SELF_ANY=3.00 PRIMER_RIGHT_325_SELF_ANY=3.00 PRIMER_LEFT_325_SELF_END=2.00 PRIMER_RIGHT_325_SELF_END=0.00 PRIMER_LEFT_325_END_STABILITY=8.2000 PRIMER_RIGHT_325_END_STABILITY=8.7000 PRIMER_PAIR_325_COMPL_ANY=3.00 PRIMER_PAIR_325_COMPL_END=1.00 PRIMER_PAIR_325_PRODUCT_SIZE=91 PRIMER_PAIR_326_PENALTY=5.548354 PRIMER_LEFT_326_PENALTY=2.605621 PRIMER_RIGHT_326_PENALTY=2.942733 PRIMER_LEFT_326_SEQUENCE=CGTTCCTGACAAGTGCAAA PRIMER_RIGHT_326_SEQUENCE=CTGCATGTCTCTTTGGCG PRIMER_LEFT_326=583,19 PRIMER_RIGHT_326=675,18 PRIMER_LEFT_326_TM=58.394 PRIMER_RIGHT_326_TM=59.057 PRIMER_LEFT_326_GC_PERCENT=47.368 PRIMER_RIGHT_326_GC_PERCENT=55.556 PRIMER_LEFT_326_SELF_ANY=4.00 PRIMER_RIGHT_326_SELF_ANY=4.00 PRIMER_LEFT_326_SELF_END=0.00 PRIMER_RIGHT_326_SELF_END=2.00 PRIMER_LEFT_326_END_STABILITY=8.8000 PRIMER_RIGHT_326_END_STABILITY=11.7000 PRIMER_PAIR_326_COMPL_ANY=4.00 PRIMER_PAIR_326_COMPL_END=2.00 PRIMER_PAIR_326_PRODUCT_SIZE=93 PRIMER_PAIR_327_PENALTY=5.554846 PRIMER_LEFT_327_PENALTY=2.600038 PRIMER_RIGHT_327_PENALTY=2.954809 PRIMER_LEFT_327_SEQUENCE=TCCCCACTTTGTGATTCCCTA PRIMER_RIGHT_327_SEQUENCE=TGCACTTGTCAGGAACGAGAA PRIMER_LEFT_327=523,21 PRIMER_RIGHT_327=599,21 PRIMER_LEFT_327_TM=61.600 PRIMER_RIGHT_327_TM=61.955 PRIMER_LEFT_327_GC_PERCENT=47.619 PRIMER_RIGHT_327_GC_PERCENT=47.619 PRIMER_LEFT_327_SELF_ANY=3.00 PRIMER_RIGHT_327_SELF_ANY=4.00 PRIMER_LEFT_327_SELF_END=3.00 PRIMER_RIGHT_327_SELF_END=1.00 PRIMER_LEFT_327_END_STABILITY=8.7000 PRIMER_RIGHT_327_END_STABILITY=6.7000 PRIMER_PAIR_327_COMPL_ANY=4.00 PRIMER_PAIR_327_COMPL_END=3.00 PRIMER_PAIR_327_PRODUCT_SIZE=77 PRIMER_PAIR_328_PENALTY=5.569766 PRIMER_LEFT_328_PENALTY=1.651669 PRIMER_RIGHT_328_PENALTY=3.918097 PRIMER_LEFT_328_SEQUENCE=TCCATCAGGGACCAAAACCT PRIMER_RIGHT_328_SEQUENCE=CTTCTACCACATTGGTGATCTGC PRIMER_LEFT_328=364,20 PRIMER_RIGHT_328=461,23 PRIMER_LEFT_328_TM=61.652 PRIMER_RIGHT_328_TM=60.918 PRIMER_LEFT_328_GC_PERCENT=50.000 PRIMER_RIGHT_328_GC_PERCENT=47.826 PRIMER_LEFT_328_SELF_ANY=5.00 PRIMER_RIGHT_328_SELF_ANY=6.00 PRIMER_LEFT_328_SELF_END=0.00 PRIMER_RIGHT_328_SELF_END=2.00 PRIMER_LEFT_328_END_STABILITY=7.9000 PRIMER_RIGHT_328_END_STABILITY=8.2000 PRIMER_PAIR_328_COMPL_ANY=5.00 PRIMER_PAIR_328_COMPL_END=0.00 PRIMER_PAIR_328_PRODUCT_SIZE=98 PRIMER_PAIR_329_PENALTY=5.604774 PRIMER_LEFT_329_PENALTY=3.368077 PRIMER_RIGHT_329_PENALTY=2.236697 PRIMER_LEFT_329_SEQUENCE=ACATGCACATGAATGTCCAGAA PRIMER_RIGHT_329_SEQUENCE=CCTTGGTATCAATGCAGGTTTT PRIMER_LEFT_329=324,22 PRIMER_RIGHT_329=398,22 PRIMER_LEFT_329_TM=61.368 PRIMER_RIGHT_329_TM=60.237 PRIMER_LEFT_329_GC_PERCENT=40.909 PRIMER_RIGHT_329_GC_PERCENT=40.909 PRIMER_LEFT_329_SELF_ANY=8.00 PRIMER_RIGHT_329_SELF_ANY=6.00 PRIMER_LEFT_329_SELF_END=3.00 PRIMER_RIGHT_329_SELF_END=0.00 PRIMER_LEFT_329_END_STABILITY=7.0000 PRIMER_RIGHT_329_END_STABILITY=7.0000 PRIMER_PAIR_329_COMPL_ANY=5.00 PRIMER_PAIR_329_COMPL_END=2.00 PRIMER_PAIR_329_PRODUCT_SIZE=75 PRIMER_PAIR_330_PENALTY=5.621018 PRIMER_LEFT_330_PENALTY=3.161089 PRIMER_RIGHT_330_PENALTY=2.459929 PRIMER_LEFT_330_SEQUENCE=CCAACCAACCAGTGACCA PRIMER_RIGHT_330_SEQUENCE=CCAACTAAGCAGCGGTAGG PRIMER_LEFT_330=462,18 PRIMER_RIGHT_330=558,19 PRIMER_LEFT_330_TM=58.839 PRIMER_RIGHT_330_TM=58.540 PRIMER_LEFT_330_GC_PERCENT=55.556 PRIMER_RIGHT_330_GC_PERCENT=57.895 PRIMER_LEFT_330_SELF_ANY=3.00 PRIMER_RIGHT_330_SELF_ANY=3.00 PRIMER_LEFT_330_SELF_END=2.00 PRIMER_RIGHT_330_SELF_END=1.00 PRIMER_LEFT_330_END_STABILITY=7.9000 PRIMER_RIGHT_330_END_STABILITY=6.9000 PRIMER_PAIR_330_COMPL_ANY=4.00 PRIMER_PAIR_330_COMPL_END=2.00 PRIMER_PAIR_330_PRODUCT_SIZE=97 PRIMER_PAIR_331_PENALTY=5.624235 PRIMER_LEFT_331_PENALTY=2.294916 PRIMER_RIGHT_331_PENALTY=3.329319 PRIMER_LEFT_331_SEQUENCE=CACCAGGAGAGGATGGATGTT PRIMER_RIGHT_331_SEQUENCE=TCTTCTCACTGCATGTCTCTTTG PRIMER_LEFT_331=608,21 PRIMER_RIGHT_331=683,23 PRIMER_LEFT_331_TM=61.295 PRIMER_RIGHT_331_TM=59.671 PRIMER_LEFT_331_GC_PERCENT=52.381 PRIMER_RIGHT_331_GC_PERCENT=43.478 PRIMER_LEFT_331_SELF_ANY=3.00 PRIMER_RIGHT_331_SELF_ANY=4.00 PRIMER_LEFT_331_SELF_END=0.00 PRIMER_RIGHT_331_SELF_END=0.00 PRIMER_LEFT_331_END_STABILITY=6.6000 PRIMER_RIGHT_331_END_STABILITY=7.3000 PRIMER_PAIR_331_COMPL_ANY=6.00 PRIMER_PAIR_331_COMPL_END=3.00 PRIMER_PAIR_331_PRODUCT_SIZE=76 PRIMER_PAIR_332_PENALTY=5.627148 PRIMER_LEFT_332_PENALTY=1.709051 PRIMER_RIGHT_332_PENALTY=3.918097 PRIMER_LEFT_332_SEQUENCE=TCCATCAGGGACCAAAACC PRIMER_RIGHT_332_SEQUENCE=GCTTCTACCACATTGGTGATCTG PRIMER_LEFT_332=364,19 PRIMER_RIGHT_332=462,23 PRIMER_LEFT_332_TM=60.709 PRIMER_RIGHT_332_TM=60.918 PRIMER_LEFT_332_GC_PERCENT=52.632 PRIMER_RIGHT_332_GC_PERCENT=47.826 PRIMER_LEFT_332_SELF_ANY=5.00 PRIMER_RIGHT_332_SELF_ANY=6.00 PRIMER_LEFT_332_SELF_END=0.00 PRIMER_RIGHT_332_SELF_END=1.00 PRIMER_LEFT_332_END_STABILITY=8.2000 PRIMER_RIGHT_332_END_STABILITY=6.6000 PRIMER_PAIR_332_COMPL_ANY=5.00 PRIMER_PAIR_332_COMPL_END=1.00 PRIMER_PAIR_332_PRODUCT_SIZE=99 PRIMER_PAIR_333_PENALTY=5.636669 PRIMER_LEFT_333_PENALTY=1.309943 PRIMER_RIGHT_333_PENALTY=4.326727 PRIMER_LEFT_333_SEQUENCE=GTCCAGAATGGGAAGTGGG PRIMER_RIGHT_333_SEQUENCE=TTCAGGGTAGACTTCTTGGCAAT PRIMER_LEFT_333=338,19 PRIMER_RIGHT_333=436,23 PRIMER_LEFT_333_TM=60.310 PRIMER_RIGHT_333_TM=61.327 PRIMER_LEFT_333_GC_PERCENT=57.895 PRIMER_RIGHT_333_GC_PERCENT=43.478 PRIMER_LEFT_333_SELF_ANY=3.00 PRIMER_RIGHT_333_SELF_ANY=5.00 PRIMER_LEFT_333_SELF_END=0.00 PRIMER_RIGHT_333_SELF_END=3.00 PRIMER_LEFT_333_END_STABILITY=9.4000 PRIMER_RIGHT_333_END_STABILITY=8.4000 PRIMER_PAIR_333_COMPL_ANY=5.00 PRIMER_PAIR_333_COMPL_END=1.00 PRIMER_PAIR_333_PRODUCT_SIZE=99 PRIMER_PAIR_334_PENALTY=5.650718 PRIMER_LEFT_334_PENALTY=3.163121 PRIMER_RIGHT_334_PENALTY=2.487598 PRIMER_LEFT_334_SEQUENCE=AACCAACCAGTGACCATCCAG PRIMER_RIGHT_334_SEQUENCE=TAAGCAGCGGTAGGGAATCAC PRIMER_LEFT_334=464,21 PRIMER_RIGHT_334=553,21 PRIMER_LEFT_334_TM=62.163 PRIMER_RIGHT_334_TM=61.488 PRIMER_LEFT_334_GC_PERCENT=52.381 PRIMER_RIGHT_334_GC_PERCENT=52.381 PRIMER_LEFT_334_SELF_ANY=3.00 PRIMER_RIGHT_334_SELF_ANY=3.00 PRIMER_LEFT_334_SELF_END=1.00 PRIMER_RIGHT_334_SELF_END=3.00 PRIMER_LEFT_334_END_STABILITY=8.2000 PRIMER_RIGHT_334_END_STABILITY=6.3000 PRIMER_PAIR_334_COMPL_ANY=4.00 PRIMER_PAIR_334_COMPL_END=1.00 PRIMER_PAIR_334_PRODUCT_SIZE=90 PRIMER_PAIR_335_PENALTY=5.655515 PRIMER_LEFT_335_PENALTY=2.782312 PRIMER_RIGHT_335_PENALTY=2.873203 PRIMER_LEFT_335_SEQUENCE=GGTGAGTTTGTAAGTGATGCCC PRIMER_RIGHT_335_SEQUENCE=TTTCGCAAACATCCATCCTCT PRIMER_LEFT_335=557,22 PRIMER_RIGHT_335=635,21 PRIMER_LEFT_335_TM=60.782 PRIMER_RIGHT_335_TM=61.873 PRIMER_LEFT_335_GC_PERCENT=50.000 PRIMER_RIGHT_335_GC_PERCENT=42.857 PRIMER_LEFT_335_SELF_ANY=2.00 PRIMER_RIGHT_335_SELF_ANY=4.00 PRIMER_LEFT_335_SELF_END=0.00 PRIMER_RIGHT_335_SELF_END=0.00 PRIMER_LEFT_335_END_STABILITY=11.2000 PRIMER_RIGHT_335_END_STABILITY=7.9000 PRIMER_PAIR_335_COMPL_ANY=5.00 PRIMER_PAIR_335_COMPL_END=1.00 PRIMER_PAIR_335_PRODUCT_SIZE=79 PRIMER_PAIR_336_PENALTY=5.664193 PRIMER_LEFT_336_PENALTY=2.247631 PRIMER_RIGHT_336_PENALTY=3.416562 PRIMER_LEFT_336_SEQUENCE=ATGTTTGCGAAACTCATCTTCA PRIMER_RIGHT_336_SEQUENCE=GGCAGCAACATGCCGTAG PRIMER_LEFT_336=624,22 PRIMER_RIGHT_336=717,18 PRIMER_LEFT_336_TM=59.752 PRIMER_RIGHT_336_TM=61.417 PRIMER_LEFT_336_GC_PERCENT=36.364 PRIMER_RIGHT_336_GC_PERCENT=61.111 PRIMER_LEFT_336_SELF_ANY=6.00 PRIMER_RIGHT_336_SELF_ANY=4.00 PRIMER_LEFT_336_SELF_END=1.00 PRIMER_RIGHT_336_SELF_END=0.00 PRIMER_LEFT_336_END_STABILITY=7.0000 PRIMER_RIGHT_336_END_STABILITY=7.4000 PRIMER_PAIR_336_COMPL_ANY=5.00 PRIMER_PAIR_336_COMPL_END=1.00 PRIMER_PAIR_336_PRODUCT_SIZE=94 PRIMER_PAIR_337_PENALTY=5.666192 PRIMER_LEFT_337_PENALTY=3.514925 PRIMER_RIGHT_337_PENALTY=2.151267 PRIMER_LEFT_337_SEQUENCE=GCTGGCTGAACCCCAGATT PRIMER_RIGHT_337_SEQUENCE=TGATGGATCTGAATCCCACT PRIMER_LEFT_337=283,19 PRIMER_RIGHT_337=370,20 PRIMER_LEFT_337_TM=62.515 PRIMER_RIGHT_337_TM=57.849 PRIMER_LEFT_337_GC_PERCENT=57.895 PRIMER_RIGHT_337_GC_PERCENT=45.000 PRIMER_LEFT_337_SELF_ANY=4.00 PRIMER_RIGHT_337_SELF_ANY=5.00 PRIMER_LEFT_337_SELF_END=1.00 PRIMER_RIGHT_337_SELF_END=1.00 PRIMER_LEFT_337_END_STABILITY=6.6000 PRIMER_RIGHT_337_END_STABILITY=7.9000 PRIMER_PAIR_337_COMPL_ANY=5.00 PRIMER_PAIR_337_COMPL_END=1.00 PRIMER_PAIR_337_PRODUCT_SIZE=88 PRIMER_PAIR_338_PENALTY=5.685447 PRIMER_LEFT_338_PENALTY=3.600004 PRIMER_RIGHT_338_PENALTY=2.085443 PRIMER_LEFT_338_SEQUENCE=GCTGAACCCCAGATTGCC PRIMER_RIGHT_338_SEQUENCE=CAGGTTTTGGTCCCTGATG PRIMER_LEFT_338=287,18 PRIMER_RIGHT_338=384,19 PRIMER_LEFT_338_TM=61.600 PRIMER_RIGHT_338_TM=58.915 PRIMER_LEFT_338_GC_PERCENT=61.111 PRIMER_RIGHT_338_GC_PERCENT=52.632 PRIMER_LEFT_338_SELF_ANY=3.00 PRIMER_RIGHT_338_SELF_ANY=4.00 PRIMER_LEFT_338_SELF_END=1.00 PRIMER_RIGHT_338_SELF_END=1.00 PRIMER_LEFT_338_END_STABILITY=10.0000 PRIMER_RIGHT_338_END_STABILITY=6.9000 PRIMER_PAIR_338_COMPL_ANY=4.00 PRIMER_PAIR_338_COMPL_END=1.00 PRIMER_PAIR_338_PRODUCT_SIZE=98 PRIMER_PAIR_339_PENALTY=5.739879 PRIMER_LEFT_339_PENALTY=2.803130 PRIMER_RIGHT_339_PENALTY=2.936750 PRIMER_LEFT_339_SEQUENCE=GTGATTCCCTACCGCTGC PRIMER_RIGHT_339_SEQUENCE=GCAAACATCCATCCTCTCCTG PRIMER_LEFT_339=533,18 PRIMER_RIGHT_339=631,21 PRIMER_LEFT_339_TM=59.197 PRIMER_RIGHT_339_TM=61.937 PRIMER_LEFT_339_GC_PERCENT=61.111 PRIMER_RIGHT_339_GC_PERCENT=52.381 PRIMER_LEFT_339_SELF_ANY=3.00 PRIMER_RIGHT_339_SELF_ANY=2.00 PRIMER_LEFT_339_SELF_END=3.00 PRIMER_RIGHT_339_SELF_END=1.00 PRIMER_LEFT_339_END_STABILITY=9.7000 PRIMER_RIGHT_339_END_STABILITY=8.2000 PRIMER_PAIR_339_COMPL_ANY=3.00 PRIMER_PAIR_339_COMPL_END=1.00 PRIMER_PAIR_339_PRODUCT_SIZE=99 PRIMER_PAIR_340_PENALTY=5.751036 PRIMER_LEFT_340_PENALTY=3.525975 PRIMER_RIGHT_340_PENALTY=2.225062 PRIMER_LEFT_340_SEQUENCE=TGGAGGTACCCACTGATGGTAA PRIMER_RIGHT_340_SEQUENCE=TTCCCATTCTGGACATTCAT PRIMER_LEFT_340=252,22 PRIMER_RIGHT_340=351,20 PRIMER_LEFT_340_TM=61.526 PRIMER_RIGHT_340_TM=57.775 PRIMER_LEFT_340_GC_PERCENT=50.000 PRIMER_RIGHT_340_GC_PERCENT=40.000 PRIMER_LEFT_340_SELF_ANY=6.00 PRIMER_RIGHT_340_SELF_ANY=3.00 PRIMER_LEFT_340_SELF_END=3.00 PRIMER_RIGHT_340_SELF_END=2.00 PRIMER_LEFT_340_END_STABILITY=7.2000 PRIMER_RIGHT_340_END_STABILITY=6.9000 PRIMER_PAIR_340_COMPL_ANY=5.00 PRIMER_PAIR_340_COMPL_END=3.00 PRIMER_PAIR_340_PRODUCT_SIZE=100 PRIMER_PAIR_341_PENALTY=5.751270 PRIMER_LEFT_341_PENALTY=3.600004 PRIMER_RIGHT_341_PENALTY=2.151267 PRIMER_LEFT_341_SEQUENCE=GGCTGAACCCCAGATTGC PRIMER_RIGHT_341_SEQUENCE=CTGATGGATCTGAATCCCAC PRIMER_LEFT_341=286,18 PRIMER_RIGHT_341=371,20 PRIMER_LEFT_341_TM=61.600 PRIMER_RIGHT_341_TM=57.849 PRIMER_LEFT_341_GC_PERCENT=61.111 PRIMER_RIGHT_341_GC_PERCENT=50.000 PRIMER_LEFT_341_SELF_ANY=3.00 PRIMER_RIGHT_341_SELF_ANY=5.00 PRIMER_LEFT_341_SELF_END=2.00 PRIMER_RIGHT_341_SELF_END=3.00 PRIMER_LEFT_341_END_STABILITY=8.4000 PRIMER_RIGHT_341_END_STABILITY=9.4000 PRIMER_PAIR_341_COMPL_ANY=5.00 PRIMER_PAIR_341_COMPL_END=0.00 PRIMER_PAIR_341_PRODUCT_SIZE=86 PRIMER_PAIR_342_PENALTY=5.753688 PRIMER_LEFT_342_PENALTY=2.646135 PRIMER_RIGHT_342_PENALTY=3.107553 PRIMER_LEFT_342_SEQUENCE=GCCCTTCTCGTTCCTGACAA PRIMER_RIGHT_342_SEQUENCE=CATGTCTCTTTGGCGACG PRIMER_LEFT_342=575,20 PRIMER_RIGHT_342=672,18 PRIMER_LEFT_342_TM=62.646 PRIMER_RIGHT_342_TM=58.892 PRIMER_LEFT_342_GC_PERCENT=55.000 PRIMER_RIGHT_342_GC_PERCENT=55.556 PRIMER_LEFT_342_SELF_ANY=3.00 PRIMER_RIGHT_342_SELF_ANY=4.00 PRIMER_LEFT_342_SELF_END=1.00 PRIMER_RIGHT_342_SELF_END=3.00 PRIMER_LEFT_342_END_STABILITY=6.7000 PRIMER_RIGHT_342_END_STABILITY=10.1000 PRIMER_PAIR_342_COMPL_ANY=4.00 PRIMER_PAIR_342_COMPL_END=2.00 PRIMER_PAIR_342_PRODUCT_SIZE=98 PRIMER_PAIR_343_PENALTY=5.767664 PRIMER_LEFT_343_PENALTY=3.220337 PRIMER_RIGHT_343_PENALTY=2.547327 PRIMER_LEFT_343_SEQUENCE=TGTGGTAGAAGCCAACCAACC PRIMER_RIGHT_343_SEQUENCE=GGATGGGTCTTGCACTGCTT PRIMER_LEFT_343=451,21 PRIMER_RIGHT_343=525,20 PRIMER_LEFT_343_TM=62.220 PRIMER_RIGHT_343_TM=62.547 PRIMER_LEFT_343_GC_PERCENT=52.381 PRIMER_RIGHT_343_GC_PERCENT=55.000 PRIMER_LEFT_343_SELF_ANY=4.00 PRIMER_RIGHT_343_SELF_ANY=5.00 PRIMER_LEFT_343_SELF_END=1.00 PRIMER_RIGHT_343_SELF_END=1.00 PRIMER_LEFT_343_END_STABILITY=8.2000 PRIMER_RIGHT_343_END_STABILITY=8.5000 PRIMER_PAIR_343_COMPL_ANY=5.00 PRIMER_PAIR_343_COMPL_END=2.00 PRIMER_PAIR_343_PRODUCT_SIZE=75 PRIMER_PAIR_344_PENALTY=5.767970 PRIMER_LEFT_344_PENALTY=3.540705 PRIMER_RIGHT_344_PENALTY=2.227266 PRIMER_LEFT_344_SEQUENCE=CCCACTGATGGTAATGCTG PRIMER_RIGHT_344_SEQUENCE=TCCCACTTCCCATTCTGGAC PRIMER_LEFT_344=260,19 PRIMER_RIGHT_344=357,20 PRIMER_LEFT_344_TM=57.459 PRIMER_RIGHT_344_TM=62.227 PRIMER_LEFT_344_GC_PERCENT=52.632 PRIMER_RIGHT_344_GC_PERCENT=55.000 PRIMER_LEFT_344_SELF_ANY=3.00 PRIMER_RIGHT_344_SELF_ANY=3.00 PRIMER_LEFT_344_SELF_END=1.00 PRIMER_RIGHT_344_SELF_END=1.00 PRIMER_LEFT_344_END_STABILITY=8.5000 PRIMER_RIGHT_344_END_STABILITY=7.9000 PRIMER_PAIR_344_COMPL_ANY=5.00 PRIMER_PAIR_344_COMPL_END=2.00 PRIMER_PAIR_344_PRODUCT_SIZE=98 PRIMER_PAIR_345_PENALTY=5.786788 PRIMER_LEFT_345_PENALTY=2.831979 PRIMER_RIGHT_345_PENALTY=2.954809 PRIMER_LEFT_345_SEQUENCE=ATCCCCACTTTGTGATTCCCT PRIMER_RIGHT_345_SEQUENCE=TTGCACTTGTCAGGAACGAGA PRIMER_LEFT_345=522,21 PRIMER_RIGHT_345=600,21 PRIMER_LEFT_345_TM=61.832 PRIMER_RIGHT_345_TM=61.955 PRIMER_LEFT_345_GC_PERCENT=47.619 PRIMER_RIGHT_345_GC_PERCENT=47.619 PRIMER_LEFT_345_SELF_ANY=4.00 PRIMER_RIGHT_345_SELF_ANY=4.00 PRIMER_LEFT_345_SELF_END=1.00 PRIMER_RIGHT_345_SELF_END=0.00 PRIMER_LEFT_345_END_STABILITY=9.4000 PRIMER_RIGHT_345_END_STABILITY=8.4000 PRIMER_PAIR_345_COMPL_ANY=4.00 PRIMER_PAIR_345_COMPL_END=2.00 PRIMER_PAIR_345_PRODUCT_SIZE=79 PRIMER_PAIR_346_PENALTY=5.790016 PRIMER_LEFT_346_PENALTY=2.782339 PRIMER_RIGHT_346_PENALTY=3.007677 PRIMER_LEFT_346_SEQUENCE=CACTTTGTGATTCCCTACCGC PRIMER_RIGHT_346_SEQUENCE=TCCATCCTCTCCTGGTGTAAGA PRIMER_LEFT_346=527,21 PRIMER_RIGHT_346=624,22 PRIMER_LEFT_346_TM=61.782 PRIMER_RIGHT_346_TM=61.008 PRIMER_LEFT_346_GC_PERCENT=52.381 PRIMER_RIGHT_346_GC_PERCENT=50.000 PRIMER_LEFT_346_SELF_ANY=3.00 PRIMER_RIGHT_346_SELF_ANY=3.00 PRIMER_LEFT_346_SELF_END=2.00 PRIMER_RIGHT_346_SELF_END=3.00 PRIMER_LEFT_346_END_STABILITY=11.1000 PRIMER_RIGHT_346_END_STABILITY=6.0000 PRIMER_PAIR_346_COMPL_ANY=3.00 PRIMER_PAIR_346_COMPL_END=1.00 PRIMER_PAIR_346_PRODUCT_SIZE=98 PRIMER_PAIR_347_PENALTY=5.793824 PRIMER_LEFT_347_PENALTY=2.577616 PRIMER_RIGHT_347_PENALTY=3.216208 PRIMER_LEFT_347_SEQUENCE=TAAGTGATGCCCTTCTCGTT PRIMER_RIGHT_347_SEQUENCE=GTGTGCCAGTGAAGATGAGTT PRIMER_LEFT_347=567,20 PRIMER_RIGHT_347=654,21 PRIMER_LEFT_347_TM=57.422 PRIMER_RIGHT_347_TM=57.784 PRIMER_LEFT_347_GC_PERCENT=45.000 PRIMER_RIGHT_347_GC_PERCENT=47.619 PRIMER_LEFT_347_SELF_ANY=4.00 PRIMER_RIGHT_347_SELF_ANY=3.00 PRIMER_LEFT_347_SELF_END=2.00 PRIMER_RIGHT_347_SELF_END=0.00 PRIMER_LEFT_347_END_STABILITY=8.4000 PRIMER_RIGHT_347_END_STABILITY=6.4000 PRIMER_PAIR_347_COMPL_ANY=4.00 PRIMER_PAIR_347_COMPL_END=0.00 PRIMER_PAIR_347_PRODUCT_SIZE=88 PRIMER_PAIR_348_PENALTY=5.818629 PRIMER_LEFT_348_PENALTY=3.218591 PRIMER_RIGHT_348_PENALTY=2.600038 PRIMER_LEFT_348_SEQUENCE=CCAACCAGTGACCATCCA PRIMER_RIGHT_348_SEQUENCE=TAGGGAATCACAAAGTGGGGA PRIMER_LEFT_348=466,18 PRIMER_RIGHT_348=543,21 PRIMER_LEFT_348_TM=58.781 PRIMER_RIGHT_348_TM=61.600 PRIMER_LEFT_348_GC_PERCENT=55.556 PRIMER_RIGHT_348_GC_PERCENT=47.619 PRIMER_LEFT_348_SELF_ANY=3.00 PRIMER_RIGHT_348_SELF_ANY=3.00 PRIMER_LEFT_348_SELF_END=0.00 PRIMER_RIGHT_348_SELF_END=2.00 PRIMER_LEFT_348_END_STABILITY=8.1000 PRIMER_RIGHT_348_END_STABILITY=10.9000 PRIMER_PAIR_348_COMPL_ANY=4.00 PRIMER_PAIR_348_COMPL_END=2.00 PRIMER_PAIR_348_PRODUCT_SIZE=78 PRIMER_PAIR_349_PENALTY=5.819733 PRIMER_LEFT_349_PENALTY=3.255899 PRIMER_RIGHT_349_PENALTY=2.563833 PRIMER_LEFT_349_SEQUENCE=CGCTGGAGGTACCCACTGA PRIMER_RIGHT_349_SEQUENCE=TGTGCATGTTCAGTCTGCCA PRIMER_LEFT_349=249,19 PRIMER_RIGHT_349=332,20 PRIMER_LEFT_349_TM=62.256 PRIMER_RIGHT_349_TM=62.564 PRIMER_LEFT_349_GC_PERCENT=63.158 PRIMER_RIGHT_349_GC_PERCENT=50.000 PRIMER_LEFT_349_SELF_ANY=6.00 PRIMER_RIGHT_349_SELF_ANY=5.00 PRIMER_LEFT_349_SELF_END=2.00 PRIMER_RIGHT_349_SELF_END=3.00 PRIMER_LEFT_349_END_STABILITY=6.4000 PRIMER_RIGHT_349_END_STABILITY=10.0000 PRIMER_PAIR_349_COMPL_ANY=5.00 PRIMER_PAIR_349_COMPL_END=2.00 PRIMER_PAIR_349_PRODUCT_SIZE=84 PRIMER_PAIR_350_PENALTY=5.821778 PRIMER_LEFT_350_PENALTY=2.601441 PRIMER_RIGHT_350_PENALTY=3.220337 PRIMER_LEFT_350_SEQUENCE=GGACCAAAACCTGCATTGATAC PRIMER_RIGHT_350_SEQUENCE=GGTTGGTTGGCTTCTACCACA PRIMER_LEFT_350=372,22 PRIMER_RIGHT_350=471,21 PRIMER_LEFT_350_TM=60.601 PRIMER_RIGHT_350_TM=62.220 PRIMER_LEFT_350_GC_PERCENT=45.455 PRIMER_RIGHT_350_GC_PERCENT=52.381 PRIMER_LEFT_350_SELF_ANY=4.00 PRIMER_RIGHT_350_SELF_ANY=4.00 PRIMER_LEFT_350_SELF_END=2.00 PRIMER_RIGHT_350_SELF_END=2.00 PRIMER_LEFT_350_END_STABILITY=5.3000 PRIMER_RIGHT_350_END_STABILITY=8.2000 PRIMER_PAIR_350_COMPL_ANY=5.00 PRIMER_PAIR_350_COMPL_END=1.00 PRIMER_PAIR_350_PRODUCT_SIZE=100 PRIMER_PAIR_351_PENALTY=5.827122 PRIMER_LEFT_351_PENALTY=1.312895 PRIMER_RIGHT_351_PENALTY=4.514227 PRIMER_LEFT_351_SEQUENCE=AGAATGGGAAGTGGGATTCAG PRIMER_RIGHT_351_SEQUENCE=AGTTCAGGGTAGACTTCTTGGCA PRIMER_LEFT_351=342,21 PRIMER_RIGHT_351=438,23 PRIMER_LEFT_351_TM=60.313 PRIMER_RIGHT_351_TM=61.514 PRIMER_LEFT_351_GC_PERCENT=47.619 PRIMER_RIGHT_351_GC_PERCENT=47.826 PRIMER_LEFT_351_SELF_ANY=4.00 PRIMER_RIGHT_351_SELF_ANY=4.00 PRIMER_LEFT_351_SELF_END=1.00 PRIMER_RIGHT_351_SELF_END=3.00 PRIMER_LEFT_351_END_STABILITY=7.0000 PRIMER_RIGHT_351_END_STABILITY=10.0000 PRIMER_PAIR_351_COMPL_ANY=5.00 PRIMER_PAIR_351_COMPL_END=0.00 PRIMER_PAIR_351_PRODUCT_SIZE=97 PRIMER_PAIR_352_PENALTY=5.832952 PRIMER_LEFT_352_PENALTY=3.220337 PRIMER_RIGHT_352_PENALTY=2.612614 PRIMER_LEFT_352_SEQUENCE=GTGGTAGAAGCCAACCAACCA PRIMER_RIGHT_352_SEQUENCE=CAAAGTGGGGATGGGTCTTG PRIMER_LEFT_352=452,21 PRIMER_RIGHT_352=533,20 PRIMER_LEFT_352_TM=62.220 PRIMER_RIGHT_352_TM=62.613 PRIMER_LEFT_352_GC_PERCENT=52.381 PRIMER_RIGHT_352_GC_PERCENT=55.000 PRIMER_LEFT_352_SELF_ANY=4.00 PRIMER_RIGHT_352_SELF_ANY=3.00 PRIMER_LEFT_352_SELF_END=1.00 PRIMER_RIGHT_352_SELF_END=0.00 PRIMER_LEFT_352_END_STABILITY=8.2000 PRIMER_RIGHT_352_END_STABILITY=7.0000 PRIMER_PAIR_352_COMPL_ANY=4.00 PRIMER_PAIR_352_COMPL_END=3.00 PRIMER_PAIR_352_PRODUCT_SIZE=82 PRIMER_PAIR_353_PENALTY=5.844497 PRIMER_LEFT_353_PENALTY=3.540705 PRIMER_RIGHT_353_PENALTY=2.303792 PRIMER_LEFT_353_SEQUENCE=CCACTGATGGTAATGCTGG PRIMER_RIGHT_353_SEQUENCE=ACTTCCCATTCTGGACATTCAT PRIMER_LEFT_353=261,19 PRIMER_RIGHT_353=353,22 PRIMER_LEFT_353_TM=57.459 PRIMER_RIGHT_353_TM=59.696 PRIMER_LEFT_353_GC_PERCENT=52.632 PRIMER_RIGHT_353_GC_PERCENT=40.909 PRIMER_LEFT_353_SELF_ANY=3.00 PRIMER_RIGHT_353_SELF_ANY=3.00 PRIMER_LEFT_353_SELF_END=0.00 PRIMER_RIGHT_353_SELF_END=2.00 PRIMER_LEFT_353_END_STABILITY=9.7000 PRIMER_RIGHT_353_END_STABILITY=6.9000 PRIMER_PAIR_353_COMPL_ANY=5.00 PRIMER_PAIR_353_COMPL_END=1.00 PRIMER_PAIR_353_PRODUCT_SIZE=93 PRIMER_PAIR_354_PENALTY=5.848511 PRIMER_LEFT_354_PENALTY=2.118737 PRIMER_RIGHT_354_PENALTY=3.729774 PRIMER_LEFT_354_SEQUENCE=AATGTCCAGAATGGGAAGTGG PRIMER_RIGHT_354_SEQUENCE=GCAGGATGCCTTCCTTGG PRIMER_LEFT_354=335,21 PRIMER_RIGHT_354=410,18 PRIMER_LEFT_354_TM=61.119 PRIMER_RIGHT_354_TM=61.730 PRIMER_LEFT_354_GC_PERCENT=47.619 PRIMER_RIGHT_354_GC_PERCENT=61.111 PRIMER_LEFT_354_SELF_ANY=3.00 PRIMER_RIGHT_354_SELF_ANY=7.00 PRIMER_LEFT_354_SELF_END=0.00 PRIMER_RIGHT_354_SELF_END=3.00 PRIMER_LEFT_354_END_STABILITY=7.9000 PRIMER_RIGHT_354_END_STABILITY=8.5000 PRIMER_PAIR_354_COMPL_ANY=5.00 PRIMER_PAIR_354_COMPL_END=0.00 PRIMER_PAIR_354_PRODUCT_SIZE=76 PRIMER_PAIR_355_PENALTY=5.866430 PRIMER_LEFT_355_PENALTY=2.631665 PRIMER_RIGHT_355_PENALTY=3.234765 PRIMER_LEFT_355_SEQUENCE=GATGCCCTTCTCGTTCCTGA PRIMER_RIGHT_355_SEQUENCE=GCCAGTGAAGATGAGTTTCGC PRIMER_LEFT_355=572,20 PRIMER_RIGHT_355=650,21 PRIMER_LEFT_355_TM=62.632 PRIMER_RIGHT_355_TM=62.235 PRIMER_LEFT_355_GC_PERCENT=55.000 PRIMER_RIGHT_355_GC_PERCENT=52.381 PRIMER_LEFT_355_SELF_ANY=2.00 PRIMER_RIGHT_355_SELF_ANY=3.00 PRIMER_LEFT_355_SELF_END=2.00 PRIMER_RIGHT_355_SELF_END=3.00 PRIMER_LEFT_355_END_STABILITY=8.2000 PRIMER_RIGHT_355_END_STABILITY=10.2000 PRIMER_PAIR_355_COMPL_ANY=4.00 PRIMER_PAIR_355_COMPL_END=0.00 PRIMER_PAIR_355_PRODUCT_SIZE=79 PRIMER_PAIR_356_PENALTY=5.889238 PRIMER_LEFT_356_PENALTY=2.840827 PRIMER_RIGHT_356_PENALTY=3.048411 PRIMER_LEFT_356_SEQUENCE=TTTGTGATTCCCTACCGCT PRIMER_RIGHT_356_SEQUENCE=TCCTGGTGTAAGAATTTGCACTT PRIMER_LEFT_356=530,19 PRIMER_RIGHT_356=615,23 PRIMER_LEFT_356_TM=58.159 PRIMER_RIGHT_356_TM=60.048 PRIMER_LEFT_356_GC_PERCENT=47.368 PRIMER_RIGHT_356_GC_PERCENT=39.130 PRIMER_LEFT_356_SELF_ANY=3.00 PRIMER_RIGHT_356_SELF_ANY=5.00 PRIMER_LEFT_356_SELF_END=2.00 PRIMER_RIGHT_356_SELF_END=2.00 PRIMER_LEFT_356_END_STABILITY=11.4000 PRIMER_RIGHT_356_END_STABILITY=6.7000 PRIMER_PAIR_356_COMPL_ANY=4.00 PRIMER_PAIR_356_COMPL_END=0.00 PRIMER_PAIR_356_PRODUCT_SIZE=86 PRIMER_PAIR_357_PENALTY=5.902417 PRIMER_LEFT_357_PENALTY=2.954809 PRIMER_RIGHT_357_PENALTY=2.947608 PRIMER_LEFT_357_SEQUENCE=TCTCGTTCCTGACAAGTGCAA PRIMER_RIGHT_357_SEQUENCE=CACTGCATGTCTCTTTGGCG PRIMER_LEFT_357=580,21 PRIMER_RIGHT_357=677,20 PRIMER_LEFT_357_TM=61.955 PRIMER_RIGHT_357_TM=62.948 PRIMER_LEFT_357_GC_PERCENT=47.619 PRIMER_RIGHT_357_GC_PERCENT=55.000 PRIMER_LEFT_357_SELF_ANY=4.00 PRIMER_RIGHT_357_SELF_ANY=4.00 PRIMER_LEFT_357_SELF_END=2.00 PRIMER_RIGHT_357_SELF_END=2.00 PRIMER_LEFT_357_END_STABILITY=8.8000 PRIMER_RIGHT_357_END_STABILITY=11.7000 PRIMER_PAIR_357_COMPL_ANY=4.00 PRIMER_PAIR_357_COMPL_END=1.00 PRIMER_PAIR_357_PRODUCT_SIZE=98 PRIMER_PAIR_358_PENALTY=5.907912 PRIMER_LEFT_358_PENALTY=2.668208 PRIMER_RIGHT_358_PENALTY=3.239704 PRIMER_LEFT_358_SEQUENCE=GTAAGTGATGCCCTTCTCGTT PRIMER_RIGHT_358_SEQUENCE=ATGAGTTTCGCAAACATCCATC PRIMER_LEFT_358=566,21 PRIMER_RIGHT_358=640,22 PRIMER_LEFT_358_TM=58.332 PRIMER_RIGHT_358_TM=61.240 PRIMER_LEFT_358_GC_PERCENT=47.619 PRIMER_RIGHT_358_GC_PERCENT=40.909 PRIMER_LEFT_358_SELF_ANY=4.00 PRIMER_RIGHT_358_SELF_ANY=6.00 PRIMER_LEFT_358_SELF_END=2.00 PRIMER_RIGHT_358_SELF_END=2.00 PRIMER_LEFT_358_END_STABILITY=8.4000 PRIMER_RIGHT_358_END_STABILITY=8.1000 PRIMER_PAIR_358_COMPL_ANY=4.00 PRIMER_PAIR_358_COMPL_END=1.00 PRIMER_PAIR_358_PRODUCT_SIZE=75 PRIMER_PAIR_359_PENALTY=5.909864 PRIMER_LEFT_359_PENALTY=2.291669 PRIMER_RIGHT_359_PENALTY=3.618195 PRIMER_LEFT_359_SEQUENCE=TGTTTGCGAAACTCATCTTCAC PRIMER_RIGHT_359_SEQUENCE=GCAGCAACATGCCGTAGT PRIMER_LEFT_359=625,22 PRIMER_RIGHT_359=716,18 PRIMER_LEFT_359_TM=60.292 PRIMER_RIGHT_359_TM=58.382 PRIMER_LEFT_359_GC_PERCENT=40.909 PRIMER_RIGHT_359_GC_PERCENT=55.556 PRIMER_LEFT_359_SELF_ANY=6.00 PRIMER_RIGHT_359_SELF_ANY=4.00 PRIMER_LEFT_359_SELF_END=3.00 PRIMER_RIGHT_359_SELF_END=1.00 PRIMER_LEFT_359_END_STABILITY=6.7000 PRIMER_RIGHT_359_END_STABILITY=5.1000 PRIMER_PAIR_359_COMPL_ANY=4.00 PRIMER_PAIR_359_COMPL_END=2.00 PRIMER_PAIR_359_PRODUCT_SIZE=92 PRIMER_PAIR_360_PENALTY=5.916750 PRIMER_LEFT_360_PENALTY=4.539189 PRIMER_RIGHT_360_PENALTY=1.377561 PRIMER_LEFT_360_SEQUENCE=GAGACATGCAGTGAGAAGAGTACC PRIMER_RIGHT_360_SEQUENCE=GTGGGCAACACACAAACTCT PRIMER_LEFT_360=665,24 PRIMER_RIGHT_360=764,20 PRIMER_LEFT_360_TM=59.461 PRIMER_RIGHT_360_TM=58.622 PRIMER_LEFT_360_GC_PERCENT=50.000 PRIMER_RIGHT_360_GC_PERCENT=50.000 PRIMER_LEFT_360_SELF_ANY=4.00 PRIMER_RIGHT_360_SELF_ANY=3.00 PRIMER_LEFT_360_SELF_END=2.00 PRIMER_RIGHT_360_SELF_END=1.00 PRIMER_LEFT_360_END_STABILITY=6.6000 PRIMER_RIGHT_360_END_STABILITY=6.1000 PRIMER_PAIR_360_COMPL_ANY=5.00 PRIMER_PAIR_360_COMPL_END=2.00 PRIMER_PAIR_360_PRODUCT_SIZE=100 PRIMER_PAIR_361_PENALTY=5.958637 PRIMER_LEFT_361_PENALTY=2.840827 PRIMER_RIGHT_361_PENALTY=3.117810 PRIMER_LEFT_361_SEQUENCE=CTTTGTGATTCCCTACCGC PRIMER_RIGHT_361_SEQUENCE=AAGAATTTGCACTTGTCAGGAA PRIMER_LEFT_361=529,19 PRIMER_RIGHT_361=606,22 PRIMER_LEFT_361_TM=58.159 PRIMER_RIGHT_361_TM=58.882 PRIMER_LEFT_361_GC_PERCENT=52.632 PRIMER_RIGHT_361_GC_PERCENT=36.364 PRIMER_LEFT_361_SELF_ANY=3.00 PRIMER_RIGHT_361_SELF_ANY=4.00 PRIMER_LEFT_361_SELF_END=2.00 PRIMER_RIGHT_361_SELF_END=0.00 PRIMER_LEFT_361_END_STABILITY=11.1000 PRIMER_RIGHT_361_END_STABILITY=8.2000 PRIMER_PAIR_361_COMPL_ANY=4.00 PRIMER_PAIR_361_COMPL_END=1.00 PRIMER_PAIR_361_PRODUCT_SIZE=78 PRIMER_PAIR_362_PENALTY=5.959057 PRIMER_LEFT_362_PENALTY=2.954879 PRIMER_RIGHT_362_PENALTY=3.004178 PRIMER_LEFT_362_SEQUENCE=GATTCCCTACCGCTGCTTAGTT PRIMER_RIGHT_362_SEQUENCE=GCAAACATCCATCCTCTCC PRIMER_LEFT_362=535,22 PRIMER_RIGHT_362=631,19 PRIMER_LEFT_362_TM=60.955 PRIMER_RIGHT_362_TM=57.996 PRIMER_LEFT_362_GC_PERCENT=50.000 PRIMER_RIGHT_362_GC_PERCENT=52.632 PRIMER_LEFT_362_SELF_ANY=3.00 PRIMER_RIGHT_362_SELF_ANY=2.00 PRIMER_LEFT_362_SELF_END=0.00 PRIMER_RIGHT_362_SELF_END=0.00 PRIMER_LEFT_362_END_STABILITY=5.7000 PRIMER_RIGHT_362_END_STABILITY=7.9000 PRIMER_PAIR_362_COMPL_ANY=3.00 PRIMER_PAIR_362_COMPL_END=0.00 PRIMER_PAIR_362_PRODUCT_SIZE=97 PRIMER_PAIR_363_PENALTY=5.964597 PRIMER_LEFT_363_PENALTY=3.363116 PRIMER_RIGHT_363_PENALTY=2.601481 PRIMER_LEFT_363_SEQUENCE=CAACCAGTGACCATCCAGAACT PRIMER_RIGHT_363_SEQUENCE=CTAAGCAGCGGTAGGGAATCA PRIMER_LEFT_363=467,22 PRIMER_RIGHT_363=554,21 PRIMER_LEFT_363_TM=61.363 PRIMER_RIGHT_363_TM=61.601 PRIMER_LEFT_363_GC_PERCENT=50.000 PRIMER_RIGHT_363_GC_PERCENT=52.381 PRIMER_LEFT_363_SELF_ANY=3.00 PRIMER_RIGHT_363_SELF_ANY=3.00 PRIMER_LEFT_363_SELF_END=2.00 PRIMER_RIGHT_363_SELF_END=1.00 PRIMER_LEFT_363_END_STABILITY=6.4000 PRIMER_RIGHT_363_END_STABILITY=6.9000 PRIMER_PAIR_363_COMPL_ANY=3.00 PRIMER_PAIR_363_COMPL_END=1.00 PRIMER_PAIR_363_PRODUCT_SIZE=88 PRIMER_PAIR_364_PENALTY=5.973566 PRIMER_LEFT_364_PENALTY=3.333224 PRIMER_RIGHT_364_PENALTY=2.640342 PRIMER_LEFT_364_SEQUENCE=TGGTAGAAGCCAACCAACCAG PRIMER_RIGHT_364_SEQUENCE=CGGTAGGGAATCACAAAGTGG PRIMER_LEFT_364=453,21 PRIMER_RIGHT_364=546,21 PRIMER_LEFT_364_TM=62.333 PRIMER_RIGHT_364_TM=61.640 PRIMER_LEFT_364_GC_PERCENT=52.381 PRIMER_RIGHT_364_GC_PERCENT=52.381 PRIMER_LEFT_364_SELF_ANY=4.00 PRIMER_RIGHT_364_SELF_ANY=3.00 PRIMER_LEFT_364_SELF_END=1.00 PRIMER_RIGHT_364_SELF_END=1.00 PRIMER_LEFT_364_END_STABILITY=7.9000 PRIMER_RIGHT_364_END_STABILITY=7.9000 PRIMER_PAIR_364_COMPL_ANY=4.00 PRIMER_PAIR_364_COMPL_END=2.00 PRIMER_PAIR_364_PRODUCT_SIZE=94 PRIMER_PAIR_365_PENALTY=6.025252 PRIMER_LEFT_365_PENALTY=4.240356 PRIMER_RIGHT_365_PENALTY=1.784896 PRIMER_LEFT_365_SEQUENCE=CAAAGAGACATGCAGTGAGAAG PRIMER_RIGHT_365_SEQUENCE=CACACAAACTCTACCCCTCG PRIMER_LEFT_365=661,22 PRIMER_RIGHT_365=756,20 PRIMER_LEFT_365_TM=57.760 PRIMER_RIGHT_365_TM=58.215 PRIMER_LEFT_365_GC_PERCENT=45.455 PRIMER_RIGHT_365_GC_PERCENT=55.000 PRIMER_LEFT_365_SELF_ANY=4.00 PRIMER_RIGHT_365_SELF_ANY=2.00 PRIMER_LEFT_365_SELF_END=0.00 PRIMER_RIGHT_365_SELF_END=2.00 PRIMER_LEFT_365_END_STABILITY=6.7000 PRIMER_RIGHT_365_END_STABILITY=9.9000 PRIMER_PAIR_365_COMPL_ANY=4.00 PRIMER_PAIR_365_COMPL_END=1.00 PRIMER_PAIR_365_PRODUCT_SIZE=96 PRIMER_PAIR_366_PENALTY=6.044911 PRIMER_LEFT_366_PENALTY=1.590836 PRIMER_RIGHT_366_PENALTY=4.454075 PRIMER_LEFT_366_SEQUENCE=CAGATCCATCAGGGACCAA PRIMER_RIGHT_366_SEQUENCE=GATCTGCAGTTCAGGGTAGACT PRIMER_LEFT_366=360,19 PRIMER_RIGHT_366=445,22 PRIMER_LEFT_366_TM=59.409 PRIMER_RIGHT_366_TM=57.546 PRIMER_LEFT_366_GC_PERCENT=52.632 PRIMER_RIGHT_366_GC_PERCENT=50.000 PRIMER_LEFT_366_SELF_ANY=5.00 PRIMER_RIGHT_366_SELF_ANY=8.00 PRIMER_LEFT_366_SELF_END=1.00 PRIMER_RIGHT_366_SELF_END=3.00 PRIMER_LEFT_366_END_STABILITY=8.2000 PRIMER_RIGHT_366_END_STABILITY=6.1000 PRIMER_PAIR_366_COMPL_ANY=6.00 PRIMER_PAIR_366_COMPL_END=1.00 PRIMER_PAIR_366_PRODUCT_SIZE=86 PRIMER_PAIR_367_PENALTY=6.053359 PRIMER_LEFT_367_PENALTY=3.430130 PRIMER_RIGHT_367_PENALTY=2.623229 PRIMER_LEFT_367_SEQUENCE=ACCATCCAGAACTGGTGCAAG PRIMER_RIGHT_367_SEQUENCE=CAACTAAGCAGCGGTAGGGAA PRIMER_LEFT_367=476,21 PRIMER_RIGHT_367=557,21 PRIMER_LEFT_367_TM=62.430 PRIMER_RIGHT_367_TM=61.623 PRIMER_LEFT_367_GC_PERCENT=52.381 PRIMER_RIGHT_367_GC_PERCENT=52.381 PRIMER_LEFT_367_SELF_ANY=6.00 PRIMER_RIGHT_367_SELF_ANY=3.00 PRIMER_LEFT_367_SELF_END=2.00 PRIMER_RIGHT_367_SELF_END=0.00 PRIMER_LEFT_367_END_STABILITY=8.5000 PRIMER_RIGHT_367_END_STABILITY=9.7000 PRIMER_PAIR_367_COMPL_ANY=4.00 PRIMER_PAIR_367_COMPL_END=1.00 PRIMER_PAIR_367_PRODUCT_SIZE=82 PRIMER_PAIR_368_PENALTY=6.058555 PRIMER_LEFT_368_PENALTY=4.183295 PRIMER_RIGHT_368_PENALTY=1.875260 PRIMER_LEFT_368_SEQUENCE=GTCGCCAAAGAGACATGC PRIMER_RIGHT_368_SEQUENCE=ACTCTACCCCTCGGAACTTGT PRIMER_LEFT_368=656,18 PRIMER_RIGHT_368=749,21 PRIMER_LEFT_368_TM=57.817 PRIMER_RIGHT_368_TM=59.125 PRIMER_LEFT_368_GC_PERCENT=55.556 PRIMER_RIGHT_368_GC_PERCENT=52.381 PRIMER_LEFT_368_SELF_ANY=4.00 PRIMER_RIGHT_368_SELF_ANY=3.00 PRIMER_LEFT_368_SELF_END=2.00 PRIMER_RIGHT_368_SELF_END=2.00 PRIMER_LEFT_368_END_STABILITY=8.4000 PRIMER_RIGHT_368_END_STABILITY=6.7000 PRIMER_PAIR_368_COMPL_ANY=4.00 PRIMER_PAIR_368_COMPL_END=2.00 PRIMER_PAIR_368_PRODUCT_SIZE=94 PRIMER_PAIR_369_PENALTY=6.070485 PRIMER_LEFT_369_PENALTY=3.883954 PRIMER_RIGHT_369_PENALTY=2.186531 PRIMER_LEFT_369_SEQUENCE=AACCCCAGATTGCCATGTTCT PRIMER_RIGHT_369_SEQUENCE=AATGCAGGTTTTGGTCCCTG PRIMER_LEFT_369=291,21 PRIMER_RIGHT_369=388,20 PRIMER_LEFT_369_TM=62.884 PRIMER_RIGHT_369_TM=62.187 PRIMER_LEFT_369_GC_PERCENT=47.619 PRIMER_RIGHT_369_GC_PERCENT=50.000 PRIMER_LEFT_369_SELF_ANY=4.00 PRIMER_RIGHT_369_SELF_ANY=4.00 PRIMER_LEFT_369_SELF_END=0.00 PRIMER_RIGHT_369_SELF_END=1.00 PRIMER_LEFT_369_END_STABILITY=6.4000 PRIMER_RIGHT_369_END_STABILITY=9.7000 PRIMER_PAIR_369_COMPL_ANY=4.00 PRIMER_PAIR_369_COMPL_END=0.00 PRIMER_PAIR_369_PRODUCT_SIZE=98 PRIMER_PAIR_370_PENALTY=6.070629 PRIMER_LEFT_370_PENALTY=3.895812 PRIMER_RIGHT_370_PENALTY=2.174817 PRIMER_LEFT_370_SEQUENCE=CCATGTTCTGTGGCAGACT PRIMER_RIGHT_370_SEQUENCE=GGTATCAATGCAGGTTTTGGT PRIMER_LEFT_370=303,19 PRIMER_RIGHT_370=394,21 PRIMER_LEFT_370_TM=57.104 PRIMER_RIGHT_370_TM=58.825 PRIMER_LEFT_370_GC_PERCENT=52.632 PRIMER_RIGHT_370_GC_PERCENT=42.857 PRIMER_LEFT_370_SELF_ANY=5.00 PRIMER_RIGHT_370_SELF_ANY=4.00 PRIMER_LEFT_370_SELF_END=3.00 PRIMER_RIGHT_370_SELF_END=2.00 PRIMER_LEFT_370_END_STABILITY=6.1000 PRIMER_RIGHT_370_END_STABILITY=8.2000 PRIMER_PAIR_370_COMPL_ANY=3.00 PRIMER_PAIR_370_COMPL_END=1.00 PRIMER_PAIR_370_PRODUCT_SIZE=92 PRIMER_PAIR_371_PENALTY=6.077946 PRIMER_LEFT_371_PENALTY=1.928663 PRIMER_RIGHT_371_PENALTY=4.149283 PRIMER_LEFT_371_SEQUENCE=TTACACCAGGAGAGGATGGA PRIMER_RIGHT_371_SEQUENCE=CGTAGTCATGCAAGTTGGTACTC PRIMER_LEFT_371=605,20 PRIMER_RIGHT_371=704,23 PRIMER_LEFT_371_TM=58.071 PRIMER_RIGHT_371_TM=58.851 PRIMER_LEFT_371_GC_PERCENT=50.000 PRIMER_RIGHT_371_GC_PERCENT=47.826 PRIMER_LEFT_371_SELF_ANY=3.00 PRIMER_RIGHT_371_SELF_ANY=5.00 PRIMER_LEFT_371_SELF_END=0.00 PRIMER_RIGHT_371_SELF_END=2.00 PRIMER_LEFT_371_END_STABILITY=8.1000 PRIMER_RIGHT_371_END_STABILITY=5.4000 PRIMER_PAIR_371_COMPL_ANY=4.00 PRIMER_PAIR_371_COMPL_END=2.00 PRIMER_PAIR_371_PRODUCT_SIZE=100 PRIMER_PAIR_372_PENALTY=6.080957 PRIMER_LEFT_372_PENALTY=4.397153 PRIMER_RIGHT_372_PENALTY=1.683804 PRIMER_LEFT_372_SEQUENCE=TTGGCACTGCTCCTGCTG PRIMER_RIGHT_372_SEQUENCE=AGAACATGGCAATCTGGGGT PRIMER_LEFT_372=212,18 PRIMER_RIGHT_372=311,20 PRIMER_LEFT_372_TM=62.397 PRIMER_RIGHT_372_TM=61.684 PRIMER_LEFT_372_GC_PERCENT=61.111 PRIMER_RIGHT_372_GC_PERCENT=50.000 PRIMER_LEFT_372_SELF_ANY=5.00 PRIMER_RIGHT_372_SELF_ANY=4.00 PRIMER_LEFT_372_SELF_END=2.00 PRIMER_RIGHT_372_SELF_END=0.00 PRIMER_LEFT_372_END_STABILITY=8.5000 PRIMER_RIGHT_372_END_STABILITY=10.6000 PRIMER_PAIR_372_COMPL_ANY=5.00 PRIMER_PAIR_372_COMPL_END=0.00 PRIMER_PAIR_372_PRODUCT_SIZE=100 PRIMER_PAIR_373_PENALTY=6.090765 PRIMER_LEFT_373_PENALTY=3.598066 PRIMER_RIGHT_373_PENALTY=2.492700 PRIMER_LEFT_373_SEQUENCE=TATTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_373_SEQUENCE=AGTTCTGGATGGTCACTGGTT PRIMER_LEFT_373=413,22 PRIMER_RIGHT_373=488,21 PRIMER_LEFT_373_TM=58.402 PRIMER_RIGHT_373_TM=58.507 PRIMER_LEFT_373_GC_PERCENT=45.455 PRIMER_RIGHT_373_GC_PERCENT=47.619 PRIMER_LEFT_373_SELF_ANY=5.00 PRIMER_RIGHT_373_SELF_ANY=3.00 PRIMER_LEFT_373_SELF_END=1.00 PRIMER_RIGHT_373_SELF_END=0.00 PRIMER_LEFT_373_END_STABILITY=9.7000 PRIMER_RIGHT_373_END_STABILITY=8.2000 PRIMER_PAIR_373_COMPL_ANY=4.00 PRIMER_PAIR_373_COMPL_END=2.00 PRIMER_PAIR_373_PRODUCT_SIZE=76 PRIMER_PAIR_374_PENALTY=6.102066 PRIMER_LEFT_374_PENALTY=3.449941 PRIMER_RIGHT_374_PENALTY=2.652125 PRIMER_LEFT_374_SEQUENCE=TAGAAGCCAACCAACCAGTGAC PRIMER_RIGHT_374_SEQUENCE=AGCAGCGGTAGGGAATCA PRIMER_LEFT_374=456,22 PRIMER_RIGHT_374=551,18 PRIMER_LEFT_374_TM=61.450 PRIMER_RIGHT_374_TM=59.348 PRIMER_LEFT_374_GC_PERCENT=50.000 PRIMER_RIGHT_374_GC_PERCENT=55.556 PRIMER_LEFT_374_SELF_ANY=3.00 PRIMER_RIGHT_374_SELF_ANY=3.00 PRIMER_LEFT_374_SELF_END=3.00 PRIMER_RIGHT_374_SELF_END=1.00 PRIMER_LEFT_374_END_STABILITY=6.1000 PRIMER_RIGHT_374_END_STABILITY=6.9000 PRIMER_PAIR_374_COMPL_ANY=4.00 PRIMER_PAIR_374_COMPL_END=2.00 PRIMER_PAIR_374_PRODUCT_SIZE=96 PRIMER_PAIR_375_PENALTY=6.109880 PRIMER_LEFT_375_PENALTY=3.007677 PRIMER_RIGHT_375_PENALTY=3.102202 PRIMER_LEFT_375_SEQUENCE=TCTTACACCAGGAGAGGATGGA PRIMER_RIGHT_375_SEQUENCE=CACTGCATGTCTCTTTGGC PRIMER_LEFT_375=603,22 PRIMER_RIGHT_375=677,19 PRIMER_LEFT_375_TM=61.008 PRIMER_RIGHT_375_TM=57.898 PRIMER_LEFT_375_GC_PERCENT=50.000 PRIMER_RIGHT_375_GC_PERCENT=52.632 PRIMER_LEFT_375_SELF_ANY=3.00 PRIMER_RIGHT_375_SELF_ANY=4.00 PRIMER_LEFT_375_SELF_END=0.00 PRIMER_RIGHT_375_SELF_END=2.00 PRIMER_LEFT_375_END_STABILITY=8.1000 PRIMER_RIGHT_375_END_STABILITY=10.0000 PRIMER_PAIR_375_COMPL_ANY=4.00 PRIMER_PAIR_375_COMPL_END=3.00 PRIMER_PAIR_375_PRODUCT_SIZE=75 PRIMER_PAIR_376_PENALTY=6.118454 PRIMER_LEFT_376_PENALTY=1.604227 PRIMER_RIGHT_376_PENALTY=4.514227 PRIMER_LEFT_376_SEQUENCE=GGGATTCAGATCCATCAGGG PRIMER_RIGHT_376_SEQUENCE=CAGTTCAGGGTAGACTTCTTGGC PRIMER_LEFT_376=354,20 PRIMER_RIGHT_376=439,23 PRIMER_LEFT_376_TM=61.604 PRIMER_RIGHT_376_TM=61.514 PRIMER_LEFT_376_GC_PERCENT=55.000 PRIMER_RIGHT_376_GC_PERCENT=52.174 PRIMER_LEFT_376_SELF_ANY=5.00 PRIMER_RIGHT_376_SELF_ANY=4.00 PRIMER_LEFT_376_SELF_END=3.00 PRIMER_RIGHT_376_SELF_END=2.00 PRIMER_LEFT_376_END_STABILITY=9.7000 PRIMER_RIGHT_376_END_STABILITY=10.0000 PRIMER_PAIR_376_COMPL_ANY=3.00 PRIMER_PAIR_376_COMPL_END=1.00 PRIMER_PAIR_376_PRODUCT_SIZE=86 PRIMER_PAIR_377_PENALTY=6.123894 PRIMER_LEFT_377_PENALTY=3.341555 PRIMER_RIGHT_377_PENALTY=2.782339 PRIMER_LEFT_377_SEQUENCE=TGTGGTAGAAGCCAACCAA PRIMER_RIGHT_377_SEQUENCE=GCGGTAGGGAATCACAAAGTG PRIMER_LEFT_377=451,19 PRIMER_RIGHT_377=547,21 PRIMER_LEFT_377_TM=57.658 PRIMER_RIGHT_377_TM=61.782 PRIMER_LEFT_377_GC_PERCENT=47.368 PRIMER_RIGHT_377_GC_PERCENT=52.381 PRIMER_LEFT_377_SELF_ANY=4.00 PRIMER_RIGHT_377_SELF_ANY=3.00 PRIMER_LEFT_377_SELF_END=2.00 PRIMER_RIGHT_377_SELF_END=3.00 PRIMER_LEFT_377_END_STABILITY=8.2000 PRIMER_RIGHT_377_END_STABILITY=6.7000 PRIMER_PAIR_377_COMPL_ANY=4.00 PRIMER_PAIR_377_COMPL_END=1.00 PRIMER_PAIR_377_PRODUCT_SIZE=97 PRIMER_PAIR_378_PENALTY=6.129218 PRIMER_LEFT_378_PENALTY=2.896506 PRIMER_RIGHT_378_PENALTY=3.232712 PRIMER_LEFT_378_SEQUENCE=CTTTGTGATTCCCTACCGCTG PRIMER_RIGHT_378_SEQUENCE=TCCTGGTGTAAGAATTTGCACT PRIMER_LEFT_378=529,21 PRIMER_RIGHT_378=615,22 PRIMER_LEFT_378_TM=61.897 PRIMER_RIGHT_378_TM=58.767 PRIMER_LEFT_378_GC_PERCENT=52.381 PRIMER_RIGHT_378_GC_PERCENT=40.909 PRIMER_LEFT_378_SELF_ANY=3.00 PRIMER_RIGHT_378_SELF_ANY=5.00 PRIMER_LEFT_378_SELF_END=2.00 PRIMER_RIGHT_378_SELF_END=3.00 PRIMER_LEFT_378_END_STABILITY=10.2000 PRIMER_RIGHT_378_END_STABILITY=7.9000 PRIMER_PAIR_378_COMPL_ANY=4.00 PRIMER_PAIR_378_COMPL_END=0.00 PRIMER_PAIR_378_PRODUCT_SIZE=87 PRIMER_PAIR_379_PENALTY=6.147891 PRIMER_LEFT_379_PENALTY=2.298898 PRIMER_RIGHT_379_PENALTY=3.848993 PRIMER_LEFT_379_SEQUENCE=GAAACTCATCTTCACTGGCACA PRIMER_RIGHT_379_SEQUENCE=ACATGCCGTAGTCATGCAAGTT PRIMER_LEFT_379=632,22 PRIMER_RIGHT_379=710,22 PRIMER_LEFT_379_TM=60.299 PRIMER_RIGHT_379_TM=61.849 PRIMER_LEFT_379_GC_PERCENT=45.455 PRIMER_RIGHT_379_GC_PERCENT=45.455 PRIMER_LEFT_379_SELF_ANY=3.00 PRIMER_RIGHT_379_SELF_ANY=6.00 PRIMER_LEFT_379_SELF_END=2.00 PRIMER_RIGHT_379_SELF_END=3.00 PRIMER_LEFT_379_END_STABILITY=8.2000 PRIMER_RIGHT_379_END_STABILITY=6.7000 PRIMER_PAIR_379_COMPL_ANY=5.00 PRIMER_PAIR_379_COMPL_END=2.00 PRIMER_PAIR_379_PRODUCT_SIZE=79 PRIMER_PAIR_380_PENALTY=6.163108 PRIMER_LEFT_380_PENALTY=1.648882 PRIMER_RIGHT_380_PENALTY=4.514227 PRIMER_LEFT_380_SEQUENCE=AAGTGGGATTCAGATCCATCA PRIMER_RIGHT_380_SEQUENCE=GCAGTTCAGGGTAGACTTCTTGG PRIMER_LEFT_380=350,21 PRIMER_RIGHT_380=440,23 PRIMER_LEFT_380_TM=59.351 PRIMER_RIGHT_380_TM=61.514 PRIMER_LEFT_380_GC_PERCENT=42.857 PRIMER_RIGHT_380_GC_PERCENT=52.174 PRIMER_LEFT_380_SELF_ANY=5.00 PRIMER_RIGHT_380_SELF_ANY=4.00 PRIMER_LEFT_380_SELF_END=2.00 PRIMER_RIGHT_380_SELF_END=0.00 PRIMER_LEFT_380_END_STABILITY=6.9000 PRIMER_RIGHT_380_END_STABILITY=8.5000 PRIMER_PAIR_380_COMPL_ANY=4.00 PRIMER_PAIR_380_COMPL_END=2.00 PRIMER_PAIR_380_PRODUCT_SIZE=91 PRIMER_PAIR_381_PENALTY=6.172759 PRIMER_LEFT_381_PENALTY=3.503107 PRIMER_RIGHT_381_PENALTY=2.669652 PRIMER_LEFT_381_SEQUENCE=GCTGGAGGTACCCACTGAT PRIMER_RIGHT_381_SEQUENCE=GTTCAGTCTGCCACAGAACA PRIMER_LEFT_381=250,19 PRIMER_RIGHT_381=325,20 PRIMER_LEFT_381_TM=57.497 PRIMER_RIGHT_381_TM=57.330 PRIMER_LEFT_381_GC_PERCENT=57.895 PRIMER_RIGHT_381_GC_PERCENT=50.000 PRIMER_LEFT_381_SELF_ANY=6.00 PRIMER_RIGHT_381_SELF_ANY=6.00 PRIMER_LEFT_381_SELF_END=2.00 PRIMER_RIGHT_381_SELF_END=2.00 PRIMER_LEFT_381_END_STABILITY=6.6000 PRIMER_RIGHT_381_END_STABILITY=6.7000 PRIMER_PAIR_381_COMPL_ANY=5.00 PRIMER_PAIR_381_COMPL_END=2.00 PRIMER_PAIR_381_PRODUCT_SIZE=76 PRIMER_PAIR_382_PENALTY=6.207793 PRIMER_LEFT_382_PENALTY=2.358800 PRIMER_RIGHT_382_PENALTY=3.848993 PRIMER_LEFT_382_SEQUENCE=TGCGAAACTCATCTTCACTGG PRIMER_RIGHT_382_SEQUENCE=AACATGCCGTAGTCATGCAAGT PRIMER_LEFT_382=629,21 PRIMER_RIGHT_382=711,22 PRIMER_LEFT_382_TM=61.359 PRIMER_RIGHT_382_TM=61.849 PRIMER_LEFT_382_GC_PERCENT=47.619 PRIMER_RIGHT_382_GC_PERCENT=45.455 PRIMER_LEFT_382_SELF_ANY=3.00 PRIMER_RIGHT_382_SELF_ANY=6.00 PRIMER_LEFT_382_SELF_END=1.00 PRIMER_RIGHT_382_SELF_END=3.00 PRIMER_LEFT_382_END_STABILITY=7.9000 PRIMER_RIGHT_382_END_STABILITY=6.7000 PRIMER_PAIR_382_COMPL_ANY=4.00 PRIMER_PAIR_382_COMPL_END=3.00 PRIMER_PAIR_382_PRODUCT_SIZE=83 PRIMER_PAIR_383_PENALTY=6.214797 PRIMER_LEFT_383_PENALTY=3.132848 PRIMER_RIGHT_383_PENALTY=3.081950 PRIMER_LEFT_383_SEQUENCE=TTGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_383_SEQUENCE=CACCAGTTCTGGATGGTCA PRIMER_LEFT_383=415,22 PRIMER_RIGHT_383=492,19 PRIMER_LEFT_383_TM=61.133 PRIMER_RIGHT_383_TM=57.918 PRIMER_LEFT_383_GC_PERCENT=45.455 PRIMER_RIGHT_383_GC_PERCENT=52.632 PRIMER_LEFT_383_SELF_ANY=5.00 PRIMER_RIGHT_383_SELF_ANY=6.00 PRIMER_LEFT_383_SELF_END=0.00 PRIMER_RIGHT_383_SELF_END=2.00 PRIMER_LEFT_383_END_STABILITY=7.0000 PRIMER_RIGHT_383_END_STABILITY=7.9000 PRIMER_PAIR_383_COMPL_ANY=4.00 PRIMER_PAIR_383_COMPL_END=3.00 PRIMER_PAIR_383_PRODUCT_SIZE=78 PRIMER_PAIR_384_PENALTY=6.218841 PRIMER_LEFT_384_PENALTY=4.341090 PRIMER_RIGHT_384_PENALTY=1.877751 PRIMER_LEFT_384_SEQUENCE=CCAAAGAGACATGCAGTGAGAAG PRIMER_RIGHT_384_SEQUENCE=ACACACAAACTCTACCCCTCG PRIMER_LEFT_384=660,23 PRIMER_RIGHT_384=757,21 PRIMER_LEFT_384_TM=61.341 PRIMER_RIGHT_384_TM=59.122 PRIMER_LEFT_384_GC_PERCENT=47.826 PRIMER_RIGHT_384_GC_PERCENT=52.381 PRIMER_LEFT_384_SELF_ANY=4.00 PRIMER_RIGHT_384_SELF_ANY=2.00 PRIMER_LEFT_384_SELF_END=0.00 PRIMER_RIGHT_384_SELF_END=2.00 PRIMER_LEFT_384_END_STABILITY=6.7000 PRIMER_RIGHT_384_END_STABILITY=9.9000 PRIMER_PAIR_384_COMPL_ANY=4.00 PRIMER_PAIR_384_COMPL_END=1.00 PRIMER_PAIR_384_PRODUCT_SIZE=98 PRIMER_PAIR_385_PENALTY=6.222255 PRIMER_LEFT_385_PENALTY=4.253992 PRIMER_RIGHT_385_PENALTY=1.968263 PRIMER_LEFT_385_SEQUENCE=TGCAGTATTGCCAAGAAGTCTA PRIMER_RIGHT_385_SEQUENCE=TCTGGATGGTCACTGGTTGG PRIMER_LEFT_385=408,22 PRIMER_RIGHT_385=485,20 PRIMER_LEFT_385_TM=57.746 PRIMER_RIGHT_385_TM=61.968 PRIMER_LEFT_385_GC_PERCENT=40.909 PRIMER_RIGHT_385_GC_PERCENT=55.000 PRIMER_LEFT_385_SELF_ANY=6.00 PRIMER_RIGHT_385_SELF_ANY=3.00 PRIMER_LEFT_385_SELF_END=2.00 PRIMER_RIGHT_385_SELF_END=0.00 PRIMER_LEFT_385_END_STABILITY=5.4000 PRIMER_RIGHT_385_END_STABILITY=8.2000 PRIMER_PAIR_385_COMPL_ANY=5.00 PRIMER_PAIR_385_COMPL_END=3.00 PRIMER_PAIR_385_PRODUCT_SIZE=78 PRIMER_PAIR_386_PENALTY=6.230821 PRIMER_LEFT_386_PENALTY=4.044290 PRIMER_RIGHT_386_PENALTY=2.186531 PRIMER_LEFT_386_SEQUENCE=ATTGCCATGTTCTGTGGC PRIMER_RIGHT_386_SEQUENCE=CAATGCAGGTTTTGGTCCCT PRIMER_LEFT_386=299,18 PRIMER_RIGHT_386=389,20 PRIMER_LEFT_386_TM=57.956 PRIMER_RIGHT_386_TM=62.187 PRIMER_LEFT_386_GC_PERCENT=50.000 PRIMER_RIGHT_386_GC_PERCENT=50.000 PRIMER_LEFT_386_SELF_ANY=4.00 PRIMER_RIGHT_386_SELF_ANY=4.00 PRIMER_LEFT_386_SELF_END=2.00 PRIMER_RIGHT_386_SELF_END=0.00 PRIMER_LEFT_386_END_STABILITY=9.4000 PRIMER_RIGHT_386_END_STABILITY=9.4000 PRIMER_PAIR_386_COMPL_ANY=4.00 PRIMER_PAIR_386_COMPL_END=0.00 PRIMER_PAIR_386_PRODUCT_SIZE=91 PRIMER_PAIR_387_PENALTY=6.235964 PRIMER_LEFT_387_PENALTY=3.668658 PRIMER_RIGHT_387_PENALTY=2.567306 PRIMER_LEFT_387_SEQUENCE=ATCACCAATGTGGTAGAAGCCA PRIMER_RIGHT_387_SEQUENCE=GGGTCTTGCACTGCTTGC PRIMER_LEFT_387=443,22 PRIMER_RIGHT_387=521,18 PRIMER_LEFT_387_TM=61.669 PRIMER_RIGHT_387_TM=60.567 PRIMER_LEFT_387_GC_PERCENT=45.455 PRIMER_RIGHT_387_GC_PERCENT=61.111 PRIMER_LEFT_387_SELF_ANY=6.00 PRIMER_RIGHT_387_SELF_ANY=5.00 PRIMER_LEFT_387_SELF_END=1.00 PRIMER_RIGHT_387_SELF_END=2.00 PRIMER_LEFT_387_END_STABILITY=9.7000 PRIMER_RIGHT_387_END_STABILITY=8.5000 PRIMER_PAIR_387_COMPL_ANY=4.00 PRIMER_PAIR_387_COMPL_END=2.00 PRIMER_PAIR_387_PRODUCT_SIZE=79 PRIMER_PAIR_388_PENALTY=6.247948 PRIMER_LEFT_388_PENALTY=2.022189 PRIMER_RIGHT_388_PENALTY=4.225759 PRIMER_LEFT_388_SEQUENCE=CACCAGGAGAGGATGGATG PRIMER_RIGHT_388_SEQUENCE=CATGCAAGTTGGTACTCTTCTCAC PRIMER_LEFT_388=608,19 PRIMER_RIGHT_388=698,24 PRIMER_LEFT_388_TM=58.978 PRIMER_RIGHT_388_TM=60.226 PRIMER_LEFT_388_GC_PERCENT=57.895 PRIMER_RIGHT_388_GC_PERCENT=45.833 PRIMER_LEFT_388_SELF_ANY=3.00 PRIMER_RIGHT_388_SELF_ANY=5.00 PRIMER_LEFT_388_SELF_END=0.00 PRIMER_RIGHT_388_SELF_END=0.00 PRIMER_LEFT_388_END_STABILITY=8.1000 PRIMER_RIGHT_388_END_STABILITY=6.4000 PRIMER_PAIR_388_COMPL_ANY=5.00 PRIMER_PAIR_388_COMPL_END=1.00 PRIMER_PAIR_388_PRODUCT_SIZE=91 PRIMER_PAIR_389_PENALTY=6.263236 PRIMER_LEFT_389_PENALTY=3.449941 PRIMER_RIGHT_389_PENALTY=2.813295 PRIMER_LEFT_389_SEQUENCE=GTAGAAGCCAACCAACCAGTGA PRIMER_RIGHT_389_SEQUENCE=AATCACAAAGTGGGGATGG PRIMER_LEFT_389=455,22 PRIMER_RIGHT_389=538,19 PRIMER_LEFT_389_TM=61.450 PRIMER_RIGHT_389_TM=58.187 PRIMER_LEFT_389_GC_PERCENT=50.000 PRIMER_RIGHT_389_GC_PERCENT=47.368 PRIMER_LEFT_389_SELF_ANY=3.00 PRIMER_RIGHT_389_SELF_ANY=4.00 PRIMER_LEFT_389_SELF_END=1.00 PRIMER_RIGHT_389_SELF_END=0.00 PRIMER_LEFT_389_END_STABILITY=6.4000 PRIMER_RIGHT_389_END_STABILITY=8.1000 PRIMER_PAIR_389_COMPL_ANY=4.00 PRIMER_PAIR_389_COMPL_END=3.00 PRIMER_PAIR_389_PRODUCT_SIZE=84 PRIMER_PAIR_390_PENALTY=6.276752 PRIMER_LEFT_390_PENALTY=3.657669 PRIMER_RIGHT_390_PENALTY=2.619082 PRIMER_LEFT_390_SEQUENCE=GCAGTATTGCCAAGAAGTCTACC PRIMER_RIGHT_390_SEQUENCE=GTTCTGGATGGTCACTGGTT PRIMER_LEFT_390=409,23 PRIMER_RIGHT_390=487,20 PRIMER_LEFT_390_TM=59.342 PRIMER_RIGHT_390_TM=57.381 PRIMER_LEFT_390_GC_PERCENT=47.826 PRIMER_RIGHT_390_GC_PERCENT=50.000 PRIMER_LEFT_390_SELF_ANY=6.00 PRIMER_RIGHT_390_SELF_ANY=3.00 PRIMER_LEFT_390_SELF_END=1.00 PRIMER_RIGHT_390_SELF_END=0.00 PRIMER_LEFT_390_END_STABILITY=6.9000 PRIMER_RIGHT_390_END_STABILITY=8.2000 PRIMER_PAIR_390_COMPL_ANY=4.00 PRIMER_PAIR_390_COMPL_END=3.00 PRIMER_PAIR_390_PRODUCT_SIZE=79 PRIMER_PAIR_391_PENALTY=6.289984 PRIMER_LEFT_391_PENALTY=3.579934 PRIMER_RIGHT_391_PENALTY=2.710050 PRIMER_LEFT_391_SEQUENCE=TCCAGAACTGGTGCAAGC PRIMER_RIGHT_391_SEQUENCE=AACTCACCAACTAAGCAGCGG PRIMER_LEFT_391=480,18 PRIMER_RIGHT_391=564,21 PRIMER_LEFT_391_TM=58.420 PRIMER_RIGHT_391_TM=61.710 PRIMER_LEFT_391_GC_PERCENT=55.556 PRIMER_RIGHT_391_GC_PERCENT=52.381 PRIMER_LEFT_391_SELF_ANY=6.00 PRIMER_RIGHT_391_SELF_ANY=3.00 PRIMER_LEFT_391_SELF_END=2.00 PRIMER_RIGHT_391_SELF_END=2.00 PRIMER_LEFT_391_END_STABILITY=8.5000 PRIMER_RIGHT_391_END_STABILITY=11.4000 PRIMER_PAIR_391_COMPL_ANY=5.00 PRIMER_PAIR_391_COMPL_END=1.00 PRIMER_PAIR_391_PRODUCT_SIZE=85 PRIMER_PAIR_392_PENALTY=6.303985 PRIMER_LEFT_392_PENALTY=3.033298 PRIMER_RIGHT_392_PENALTY=3.270687 PRIMER_LEFT_392_SEQUENCE=TGTAAGTGATGCCCTTCTCGTT PRIMER_RIGHT_392_SEQUENCE=ACGGTGTGCCAGTGAAGA PRIMER_LEFT_392=565,22 PRIMER_RIGHT_392=657,18 PRIMER_LEFT_392_TM=61.033 PRIMER_RIGHT_392_TM=58.729 PRIMER_LEFT_392_GC_PERCENT=45.455 PRIMER_RIGHT_392_GC_PERCENT=55.556 PRIMER_LEFT_392_SELF_ANY=4.00 PRIMER_RIGHT_392_SELF_ANY=3.00 PRIMER_LEFT_392_SELF_END=2.00 PRIMER_RIGHT_392_SELF_END=0.00 PRIMER_LEFT_392_END_STABILITY=8.4000 PRIMER_RIGHT_392_END_STABILITY=6.7000 PRIMER_PAIR_392_COMPL_ANY=4.00 PRIMER_PAIR_392_COMPL_END=2.00 PRIMER_PAIR_392_PRODUCT_SIZE=93 PRIMER_PAIR_393_PENALTY=6.303985 PRIMER_LEFT_393_PENALTY=3.033298 PRIMER_RIGHT_393_PENALTY=3.270687 PRIMER_LEFT_393_SEQUENCE=TTGTAAGTGATGCCCTTCTCGT PRIMER_RIGHT_393_SEQUENCE=GACGGTGTGCCAGTGAAG PRIMER_LEFT_393=564,22 PRIMER_RIGHT_393=658,18 PRIMER_LEFT_393_TM=61.033 PRIMER_RIGHT_393_TM=58.729 PRIMER_LEFT_393_GC_PERCENT=45.455 PRIMER_RIGHT_393_GC_PERCENT=61.111 PRIMER_LEFT_393_SELF_ANY=4.00 PRIMER_RIGHT_393_SELF_ANY=3.00 PRIMER_LEFT_393_SELF_END=2.00 PRIMER_RIGHT_393_SELF_END=0.00 PRIMER_LEFT_393_END_STABILITY=8.1000 PRIMER_RIGHT_393_END_STABILITY=7.0000 PRIMER_PAIR_393_COMPL_ANY=4.00 PRIMER_PAIR_393_COMPL_END=3.00 PRIMER_PAIR_393_PRODUCT_SIZE=95 PRIMER_PAIR_394_PENALTY=6.314354 PRIMER_LEFT_394_PENALTY=3.057917 PRIMER_RIGHT_394_PENALTY=3.256437 PRIMER_LEFT_394_SEQUENCE=CCTACCGCTGCTTAGTTGGTG PRIMER_RIGHT_394_SEQUENCE=CATCCATCCTCTCCTGGTGTAA PRIMER_LEFT_394=540,21 PRIMER_RIGHT_394=626,22 PRIMER_LEFT_394_TM=62.058 PRIMER_RIGHT_394_TM=61.256 PRIMER_LEFT_394_GC_PERCENT=57.143 PRIMER_RIGHT_394_GC_PERCENT=50.000 PRIMER_LEFT_394_SELF_ANY=3.00 PRIMER_RIGHT_394_SELF_ANY=3.00 PRIMER_LEFT_394_SELF_END=0.00 PRIMER_RIGHT_394_SELF_END=1.00 PRIMER_LEFT_394_END_STABILITY=8.2000 PRIMER_RIGHT_394_END_STABILITY=6.0000 PRIMER_PAIR_394_COMPL_ANY=3.00 PRIMER_PAIR_394_COMPL_END=0.00 PRIMER_PAIR_394_PRODUCT_SIZE=87 PRIMER_PAIR_395_PENALTY=6.335765 PRIMER_LEFT_395_PENALTY=2.358800 PRIMER_RIGHT_395_PENALTY=3.976964 PRIMER_LEFT_395_SEQUENCE=CGAAACTCATCTTCACTGGCA PRIMER_RIGHT_395_SEQUENCE=CAACATGCCGTAGTCATGCAA PRIMER_LEFT_395=631,21 PRIMER_RIGHT_395=712,21 PRIMER_LEFT_395_TM=61.359 PRIMER_RIGHT_395_TM=62.977 PRIMER_LEFT_395_GC_PERCENT=47.619 PRIMER_RIGHT_395_GC_PERCENT=47.619 PRIMER_LEFT_395_SELF_ANY=3.00 PRIMER_RIGHT_395_SELF_ANY=6.00 PRIMER_LEFT_395_SELF_END=3.00 PRIMER_RIGHT_395_SELF_END=2.00 PRIMER_LEFT_395_END_STABILITY=10.0000 PRIMER_RIGHT_395_END_STABILITY=8.8000 PRIMER_PAIR_395_COMPL_ANY=4.00 PRIMER_PAIR_395_COMPL_END=3.00 PRIMER_PAIR_395_PRODUCT_SIZE=82 PRIMER_PAIR_396_PENALTY=6.337271 PRIMER_LEFT_396_PENALTY=4.354763 PRIMER_RIGHT_396_PENALTY=1.982507 PRIMER_LEFT_396_SEQUENCE=ATCCTGCAGTATTGCCAAGAAGT PRIMER_RIGHT_396_SEQUENCE=GGTCACTGGTTGGTTGGCT PRIMER_LEFT_396=404,23 PRIMER_RIGHT_396=478,19 PRIMER_LEFT_396_TM=61.355 PRIMER_RIGHT_396_TM=60.983 PRIMER_LEFT_396_GC_PERCENT=43.478 PRIMER_RIGHT_396_GC_PERCENT=57.895 PRIMER_LEFT_396_SELF_ANY=8.00 PRIMER_RIGHT_396_SELF_ANY=3.00 PRIMER_LEFT_396_SELF_END=2.00 PRIMER_RIGHT_396_SELF_END=0.00 PRIMER_LEFT_396_END_STABILITY=6.4000 PRIMER_RIGHT_396_END_STABILITY=9.7000 PRIMER_PAIR_396_COMPL_ANY=5.00 PRIMER_PAIR_396_COMPL_END=1.00 PRIMER_PAIR_396_PRODUCT_SIZE=75 PRIMER_PAIR_397_PENALTY=6.339262 PRIMER_LEFT_397_PENALTY=1.676675 PRIMER_RIGHT_397_PENALTY=4.662587 PRIMER_LEFT_397_SEQUENCE=GGATTCAGATCCATCAGGGAC PRIMER_RIGHT_397_SEQUENCE=GATCTGCAGTTCAGGGTAGACTTC PRIMER_LEFT_397=355,21 PRIMER_RIGHT_397=445,24 PRIMER_LEFT_397_TM=60.677 PRIMER_RIGHT_397_TM=60.663 PRIMER_LEFT_397_GC_PERCENT=52.381 PRIMER_RIGHT_397_GC_PERCENT=50.000 PRIMER_LEFT_397_SELF_ANY=5.00 PRIMER_RIGHT_397_SELF_ANY=8.00 PRIMER_LEFT_397_SELF_END=3.00 PRIMER_RIGHT_397_SELF_END=3.00 PRIMER_LEFT_397_END_STABILITY=9.1000 PRIMER_RIGHT_397_END_STABILITY=6.4000 PRIMER_PAIR_397_COMPL_ANY=6.00 PRIMER_PAIR_397_COMPL_END=1.00 PRIMER_PAIR_397_PRODUCT_SIZE=91 PRIMER_PAIR_398_PENALTY=6.354506 PRIMER_LEFT_398_PENALTY=3.776889 PRIMER_RIGHT_398_PENALTY=2.577616 PRIMER_LEFT_398_SEQUENCE=CGCAAGCAGTGCAAGACC PRIMER_RIGHT_398_SEQUENCE=AACGAGAAGGGCATCACTTA PRIMER_LEFT_398=503,18 PRIMER_RIGHT_398=586,20 PRIMER_LEFT_398_TM=61.777 PRIMER_RIGHT_398_TM=57.422 PRIMER_LEFT_398_GC_PERCENT=61.111 PRIMER_RIGHT_398_GC_PERCENT=45.000 PRIMER_LEFT_398_SELF_ANY=5.00 PRIMER_RIGHT_398_SELF_ANY=4.00 PRIMER_LEFT_398_SELF_END=0.00 PRIMER_RIGHT_398_SELF_END=2.00 PRIMER_LEFT_398_END_STABILITY=7.6000 PRIMER_RIGHT_398_END_STABILITY=5.7000 PRIMER_PAIR_398_COMPL_ANY=4.00 PRIMER_PAIR_398_COMPL_END=2.00 PRIMER_PAIR_398_PRODUCT_SIZE=84 PRIMER_PAIR_399_PENALTY=6.367331 PRIMER_LEFT_399_PENALTY=3.070814 PRIMER_RIGHT_399_PENALTY=3.296517 PRIMER_LEFT_399_SEQUENCE=GATTCCCTACCGCTGCTTA PRIMER_RIGHT_399_SEQUENCE=CTCCTGGTGTAAGAATTTGCACT PRIMER_LEFT_399=535,19 PRIMER_RIGHT_399=616,23 PRIMER_LEFT_399_TM=57.929 PRIMER_RIGHT_399_TM=59.703 PRIMER_LEFT_399_GC_PERCENT=52.632 PRIMER_RIGHT_399_GC_PERCENT=43.478 PRIMER_LEFT_399_SELF_ANY=3.00 PRIMER_RIGHT_399_SELF_ANY=5.00 PRIMER_LEFT_399_SELF_END=2.00 PRIMER_RIGHT_399_SELF_END=3.00 PRIMER_LEFT_399_END_STABILITY=7.5000 PRIMER_RIGHT_399_END_STABILITY=7.9000 PRIMER_PAIR_399_COMPL_ANY=4.00 PRIMER_PAIR_399_COMPL_END=1.00 PRIMER_PAIR_399_PRODUCT_SIZE=82 PRIMER_PAIR_400_PENALTY=6.369600 PRIMER_LEFT_400_PENALTY=3.925274 PRIMER_RIGHT_400_PENALTY=2.444325 PRIMER_LEFT_400_SEQUENCE=GACCAAAACCTGCATTGATAC PRIMER_RIGHT_400_SEQUENCE=TGGTTGGTTGGCTTCTACC PRIMER_LEFT_400=373,21 PRIMER_RIGHT_400=472,19 PRIMER_LEFT_400_TM=57.075 PRIMER_RIGHT_400_TM=58.556 PRIMER_LEFT_400_GC_PERCENT=42.857 PRIMER_RIGHT_400_GC_PERCENT=52.632 PRIMER_LEFT_400_SELF_ANY=4.00 PRIMER_RIGHT_400_SELF_ANY=3.00 PRIMER_LEFT_400_SELF_END=2.00 PRIMER_RIGHT_400_SELF_END=2.00 PRIMER_LEFT_400_END_STABILITY=5.3000 PRIMER_RIGHT_400_END_STABILITY=6.9000 PRIMER_PAIR_400_COMPL_ANY=5.00 PRIMER_PAIR_400_COMPL_END=1.00 PRIMER_PAIR_400_PRODUCT_SIZE=100 PRIMER_PAIR_401_PENALTY=6.394823 PRIMER_LEFT_401_PENALTY=3.612510 PRIMER_RIGHT_401_PENALTY=2.782312 PRIMER_LEFT_401_SEQUENCE=CAGAACTGGTGCAAGCGG PRIMER_RIGHT_401_SEQUENCE=GGGCATCACTTACAAACTCACC PRIMER_LEFT_401=482,18 PRIMER_RIGHT_401=578,22 PRIMER_LEFT_401_TM=61.613 PRIMER_RIGHT_401_TM=60.782 PRIMER_LEFT_401_GC_PERCENT=61.111 PRIMER_RIGHT_401_GC_PERCENT=50.000 PRIMER_LEFT_401_SELF_ANY=4.00 PRIMER_RIGHT_401_SELF_ANY=2.00 PRIMER_LEFT_401_SELF_END=1.00 PRIMER_RIGHT_401_SELF_END=0.00 PRIMER_LEFT_401_END_STABILITY=11.4000 PRIMER_RIGHT_401_END_STABILITY=7.9000 PRIMER_PAIR_401_COMPL_ANY=5.00 PRIMER_PAIR_401_COMPL_END=2.00 PRIMER_PAIR_401_PRODUCT_SIZE=97 PRIMER_PAIR_402_PENALTY=6.403542 PRIMER_LEFT_402_PENALTY=1.543091 PRIMER_RIGHT_402_PENALTY=4.860451 PRIMER_LEFT_402_SEQUENCE=TGTCCAGAATGGGAAGTGG PRIMER_RIGHT_402_SEQUENCE=TCAGGGTAGACTTCTTGGCAATAC PRIMER_LEFT_402=337,19 PRIMER_RIGHT_402=435,24 PRIMER_LEFT_402_TM=59.457 PRIMER_RIGHT_402_TM=60.860 PRIMER_LEFT_402_GC_PERCENT=52.632 PRIMER_RIGHT_402_GC_PERCENT=45.833 PRIMER_LEFT_402_SELF_ANY=3.00 PRIMER_RIGHT_402_SELF_ANY=5.00 PRIMER_LEFT_402_SELF_END=0.00 PRIMER_RIGHT_402_SELF_END=3.00 PRIMER_LEFT_402_END_STABILITY=7.9000 PRIMER_RIGHT_402_END_STABILITY=5.6000 PRIMER_PAIR_402_COMPL_ANY=5.00 PRIMER_PAIR_402_COMPL_END=1.00 PRIMER_PAIR_402_PRODUCT_SIZE=99 PRIMER_PAIR_403_PENALTY=6.445098 PRIMER_LEFT_403_PENALTY=3.776889 PRIMER_RIGHT_403_PENALTY=2.668208 PRIMER_LEFT_403_SEQUENCE=CCGCAAGCAGTGCAAGAC PRIMER_RIGHT_403_SEQUENCE=AACGAGAAGGGCATCACTTAC PRIMER_LEFT_403=502,18 PRIMER_RIGHT_403=586,21 PRIMER_LEFT_403_TM=61.777 PRIMER_RIGHT_403_TM=58.332 PRIMER_LEFT_403_GC_PERCENT=61.111 PRIMER_RIGHT_403_GC_PERCENT=47.619 PRIMER_LEFT_403_SELF_ANY=5.00 PRIMER_RIGHT_403_SELF_ANY=4.00 PRIMER_LEFT_403_SELF_END=1.00 PRIMER_RIGHT_403_SELF_END=2.00 PRIMER_LEFT_403_END_STABILITY=6.4000 PRIMER_RIGHT_403_END_STABILITY=5.7000 PRIMER_PAIR_403_COMPL_ANY=4.00 PRIMER_PAIR_403_COMPL_END=1.00 PRIMER_PAIR_403_PRODUCT_SIZE=85 PRIMER_PAIR_404_PENALTY=6.445296 PRIMER_LEFT_404_PENALTY=4.021939 PRIMER_RIGHT_404_PENALTY=2.423358 PRIMER_LEFT_404_SEQUENCE=TACCCACTGATGGTAATGCTGG PRIMER_RIGHT_404_SEQUENCE=TCCCACTTCCCATTCTGGA PRIMER_LEFT_404=258,22 PRIMER_RIGHT_404=357,19 PRIMER_LEFT_404_TM=62.022 PRIMER_RIGHT_404_TM=61.423 PRIMER_LEFT_404_GC_PERCENT=50.000 PRIMER_RIGHT_404_GC_PERCENT=52.632 PRIMER_LEFT_404_SELF_ANY=5.00 PRIMER_RIGHT_404_SELF_ANY=3.00 PRIMER_LEFT_404_SELF_END=0.00 PRIMER_RIGHT_404_SELF_END=2.00 PRIMER_LEFT_404_END_STABILITY=9.7000 PRIMER_RIGHT_404_END_STABILITY=8.2000 PRIMER_PAIR_404_COMPL_ANY=5.00 PRIMER_PAIR_404_COMPL_END=3.00 PRIMER_PAIR_404_PRODUCT_SIZE=100 PRIMER_PAIR_405_PENALTY=6.445538 PRIMER_LEFT_405_PENALTY=4.381939 PRIMER_RIGHT_405_PENALTY=2.063598 PRIMER_LEFT_405_SEQUENCE=GCATTGATACCAAGGAAGGCAT PRIMER_RIGHT_405_SEQUENCE=GGTCACTGGTTGGTTGGC PRIMER_LEFT_405=384,22 PRIMER_RIGHT_405=478,18 PRIMER_LEFT_405_TM=62.382 PRIMER_RIGHT_405_TM=59.936 PRIMER_LEFT_405_GC_PERCENT=45.455 PRIMER_RIGHT_405_GC_PERCENT=61.111 PRIMER_LEFT_405_SELF_ANY=6.00 PRIMER_RIGHT_405_SELF_ANY=3.00 PRIMER_LEFT_405_SELF_END=2.00 PRIMER_RIGHT_405_SELF_END=2.00 PRIMER_LEFT_405_END_STABILITY=9.6000 PRIMER_RIGHT_405_END_STABILITY=10.0000 PRIMER_PAIR_405_COMPL_ANY=5.00 PRIMER_PAIR_405_COMPL_END=1.00 PRIMER_PAIR_405_PRODUCT_SIZE=95 PRIMER_PAIR_406_PENALTY=6.451251 PRIMER_LEFT_406_PENALTY=3.244134 PRIMER_RIGHT_406_PENALTY=3.207117 PRIMER_LEFT_406_SEQUENCE=GCAAGACCCATCCCCACTT PRIMER_RIGHT_406_SEQUENCE=GAACGAGAAGGGCATCACT PRIMER_LEFT_406=513,19 PRIMER_RIGHT_406=587,19 PRIMER_LEFT_406_TM=62.244 PRIMER_RIGHT_406_TM=57.793 PRIMER_LEFT_406_GC_PERCENT=57.895 PRIMER_RIGHT_406_GC_PERCENT=52.632 PRIMER_LEFT_406_SELF_ANY=3.00 PRIMER_RIGHT_406_SELF_ANY=2.00 PRIMER_LEFT_406_SELF_END=0.00 PRIMER_RIGHT_406_SELF_END=2.00 PRIMER_LEFT_406_END_STABILITY=6.7000 PRIMER_RIGHT_406_END_STABILITY=6.4000 PRIMER_PAIR_406_COMPL_ANY=4.00 PRIMER_PAIR_406_COMPL_END=0.00 PRIMER_PAIR_406_PRODUCT_SIZE=75 PRIMER_PAIR_407_PENALTY=6.485511 PRIMER_LEFT_407_PENALTY=3.173109 PRIMER_RIGHT_407_PENALTY=3.312402 PRIMER_LEFT_407_SEQUENCE=TGTGATTCCCTACCGCTGC PRIMER_RIGHT_407_SEQUENCE=AAGAATTTGCACTTGTCAGGAAC PRIMER_LEFT_407=532,19 PRIMER_RIGHT_407=606,23 PRIMER_LEFT_407_TM=62.173 PRIMER_RIGHT_407_TM=59.688 PRIMER_LEFT_407_GC_PERCENT=57.895 PRIMER_RIGHT_407_GC_PERCENT=39.130 PRIMER_LEFT_407_SELF_ANY=3.00 PRIMER_RIGHT_407_SELF_ANY=4.00 PRIMER_LEFT_407_SELF_END=3.00 PRIMER_RIGHT_407_SELF_END=1.00 PRIMER_LEFT_407_END_STABILITY=9.7000 PRIMER_RIGHT_407_END_STABILITY=7.9000 PRIMER_PAIR_407_COMPL_ANY=4.00 PRIMER_PAIR_407_COMPL_END=3.00 PRIMER_PAIR_407_PRODUCT_SIZE=75 PRIMER_PAIR_408_PENALTY=6.510655 PRIMER_LEFT_408_PENALTY=3.048411 PRIMER_RIGHT_408_PENALTY=3.462244 PRIMER_LEFT_408_SEQUENCE=AAGTGCAAATTCTTACACCAGGA PRIMER_RIGHT_408_SEQUENCE=CTCTTTGGCGACGGTGTG PRIMER_LEFT_408=593,23 PRIMER_RIGHT_408=667,18 PRIMER_LEFT_408_TM=60.048 PRIMER_RIGHT_408_TM=61.462 PRIMER_LEFT_408_GC_PERCENT=39.130 PRIMER_RIGHT_408_GC_PERCENT=61.111 PRIMER_LEFT_408_SELF_ANY=5.00 PRIMER_RIGHT_408_SELF_ANY=3.00 PRIMER_LEFT_408_SELF_END=1.00 PRIMER_RIGHT_408_SELF_END=0.00 PRIMER_LEFT_408_END_STABILITY=8.2000 PRIMER_RIGHT_408_END_STABILITY=6.4000 PRIMER_PAIR_408_COMPL_ANY=5.00 PRIMER_PAIR_408_COMPL_END=1.00 PRIMER_PAIR_408_PRODUCT_SIZE=75 PRIMER_PAIR_409_PENALTY=6.516191 PRIMER_LEFT_409_PENALTY=4.599420 PRIMER_RIGHT_409_PENALTY=1.916771 PRIMER_LEFT_409_SEQUENCE=CAAAGAGACATGCAGTGAGAAGAG PRIMER_RIGHT_409_SEQUENCE=ACACAAACTCTACCCCTCGGA PRIMER_LEFT_409=661,24 PRIMER_RIGHT_409=755,21 PRIMER_LEFT_409_TM=60.599 PRIMER_RIGHT_409_TM=60.917 PRIMER_LEFT_409_GC_PERCENT=45.833 PRIMER_RIGHT_409_GC_PERCENT=52.381 PRIMER_LEFT_409_SELF_ANY=4.00 PRIMER_RIGHT_409_SELF_ANY=3.00 PRIMER_LEFT_409_SELF_END=0.00 PRIMER_RIGHT_409_SELF_END=3.00 PRIMER_LEFT_409_END_STABILITY=6.7000 PRIMER_RIGHT_409_END_STABILITY=9.9000 PRIMER_PAIR_409_COMPL_ANY=4.00 PRIMER_PAIR_409_COMPL_END=3.00 PRIMER_PAIR_409_PRODUCT_SIZE=95 PRIMER_PAIR_410_PENALTY=6.536925 PRIMER_LEFT_410_PENALTY=4.244134 PRIMER_RIGHT_410_PENALTY=2.292791 PRIMER_LEFT_410_SEQUENCE=GCAGACTGAACATGCACATGAA PRIMER_RIGHT_410_SEQUENCE=CAATGCAGGTTTTGGTCCC PRIMER_LEFT_410=315,22 PRIMER_RIGHT_410=389,19 PRIMER_LEFT_410_TM=62.244 PRIMER_RIGHT_410_TM=61.293 PRIMER_LEFT_410_GC_PERCENT=45.455 PRIMER_RIGHT_410_GC_PERCENT=52.632 PRIMER_LEFT_410_SELF_ANY=6.00 PRIMER_RIGHT_410_SELF_ANY=4.00 PRIMER_LEFT_410_SELF_END=2.00 PRIMER_RIGHT_410_SELF_END=2.00 PRIMER_LEFT_410_END_STABILITY=6.9000 PRIMER_RIGHT_410_END_STABILITY=9.1000 PRIMER_PAIR_410_COMPL_ANY=6.00 PRIMER_PAIR_410_COMPL_END=2.00 PRIMER_PAIR_410_PRODUCT_SIZE=75 PRIMER_PAIR_411_PENALTY=6.538057 PRIMER_LEFT_411_PENALTY=3.690586 PRIMER_RIGHT_411_PENALTY=2.847471 PRIMER_LEFT_411_SEQUENCE=CACCAATGTGGTAGAAGCCAAC PRIMER_RIGHT_411_SEQUENCE=CACAAAGTGGGGATGGGT PRIMER_LEFT_411=445,22 PRIMER_RIGHT_411=535,18 PRIMER_LEFT_411_TM=61.691 PRIMER_RIGHT_411_TM=59.153 PRIMER_LEFT_411_GC_PERCENT=50.000 PRIMER_RIGHT_411_GC_PERCENT=55.556 PRIMER_LEFT_411_SELF_ANY=6.00 PRIMER_RIGHT_411_SELF_ANY=3.00 PRIMER_LEFT_411_SELF_END=0.00 PRIMER_RIGHT_411_SELF_END=0.00 PRIMER_LEFT_411_END_STABILITY=8.2000 PRIMER_RIGHT_411_END_STABILITY=9.4000 PRIMER_PAIR_411_COMPL_ANY=4.00 PRIMER_PAIR_411_COMPL_END=3.00 PRIMER_PAIR_411_PRODUCT_SIZE=91 PRIMER_PAIR_412_PENALTY=6.559830 PRIMER_LEFT_412_PENALTY=3.756700 PRIMER_RIGHT_412_PENALTY=2.803130 PRIMER_LEFT_412_SEQUENCE=ACCAACCAGTGACCATCCAGA PRIMER_RIGHT_412_SEQUENCE=GCAGCGGTAGGGAATCAC PRIMER_LEFT_412=465,21 PRIMER_RIGHT_412=550,18 PRIMER_LEFT_412_TM=62.757 PRIMER_RIGHT_412_TM=59.197 PRIMER_LEFT_412_GC_PERCENT=52.381 PRIMER_RIGHT_412_GC_PERCENT=61.111 PRIMER_LEFT_412_SELF_ANY=3.00 PRIMER_RIGHT_412_SELF_ANY=3.00 PRIMER_LEFT_412_SELF_END=2.00 PRIMER_RIGHT_412_SELF_END=3.00 PRIMER_LEFT_412_END_STABILITY=8.2000 PRIMER_RIGHT_412_END_STABILITY=6.3000 PRIMER_PAIR_412_COMPL_ANY=4.00 PRIMER_PAIR_412_COMPL_END=1.00 PRIMER_PAIR_412_PRODUCT_SIZE=86 PRIMER_PAIR_413_PENALTY=6.564387 PRIMER_LEFT_413_PENALTY=1.757583 PRIMER_RIGHT_413_PENALTY=4.806804 PRIMER_LEFT_413_SEQUENCE=ATGGGAAGTGGGATTCAGAT PRIMER_RIGHT_413_SEQUENCE=TCTGCAGTTCAGGGTAGACTTCTT PRIMER_LEFT_413=345,20 PRIMER_RIGHT_413=443,24 PRIMER_LEFT_413_TM=58.242 PRIMER_RIGHT_413_TM=60.807 PRIMER_LEFT_413_GC_PERCENT=45.000 PRIMER_RIGHT_413_GC_PERCENT=45.833 PRIMER_LEFT_413_SELF_ANY=3.00 PRIMER_RIGHT_413_SELF_ANY=6.00 PRIMER_LEFT_413_SELF_END=2.00 PRIMER_RIGHT_413_SELF_END=2.00 PRIMER_LEFT_413_END_STABILITY=6.6000 PRIMER_RIGHT_413_END_STABILITY=7.0000 PRIMER_PAIR_413_COMPL_ANY=5.00 PRIMER_PAIR_413_COMPL_END=1.00 PRIMER_PAIR_413_PRODUCT_SIZE=99 PRIMER_PAIR_414_PENALTY=6.566269 PRIMER_LEFT_414_PENALTY=3.205809 PRIMER_RIGHT_414_PENALTY=3.360461 PRIMER_LEFT_414_SEQUENCE=TACCGCTGCTTAGTTGGTGAGT PRIMER_RIGHT_414_SEQUENCE=TCCTCTCCTGGTGTAAGAATTTG PRIMER_LEFT_414=542,22 PRIMER_RIGHT_414=620,23 PRIMER_LEFT_414_TM=61.206 PRIMER_RIGHT_414_TM=59.640 PRIMER_LEFT_414_GC_PERCENT=50.000 PRIMER_RIGHT_414_GC_PERCENT=43.478 PRIMER_LEFT_414_SELF_ANY=3.00 PRIMER_RIGHT_414_SELF_ANY=4.00 PRIMER_LEFT_414_SELF_END=1.00 PRIMER_RIGHT_414_SELF_END=1.00 PRIMER_LEFT_414_END_STABILITY=6.4000 PRIMER_RIGHT_414_END_STABILITY=7.2000 PRIMER_PAIR_414_COMPL_ANY=4.00 PRIMER_PAIR_414_COMPL_END=0.00 PRIMER_PAIR_414_PRODUCT_SIZE=79 PRIMER_PAIR_415_PENALTY=6.567389 PRIMER_LEFT_415_PENALTY=3.612510 PRIMER_RIGHT_415_PENALTY=2.954879 PRIMER_LEFT_415_SEQUENCE=CCAGAACTGGTGCAAGCG PRIMER_RIGHT_415_SEQUENCE=AACTAAGCAGCGGTAGGGAATC PRIMER_LEFT_415=481,18 PRIMER_RIGHT_415=556,22 PRIMER_LEFT_415_TM=61.613 PRIMER_RIGHT_415_TM=60.955 PRIMER_LEFT_415_GC_PERCENT=61.111 PRIMER_RIGHT_415_GC_PERCENT=50.000 PRIMER_LEFT_415_SELF_ANY=6.00 PRIMER_RIGHT_415_SELF_ANY=3.00 PRIMER_LEFT_415_SELF_END=2.00 PRIMER_RIGHT_415_SELF_END=3.00 PRIMER_LEFT_415_END_STABILITY=10.2000 PRIMER_RIGHT_415_END_STABILITY=6.6000 PRIMER_PAIR_415_COMPL_ANY=4.00 PRIMER_PAIR_415_COMPL_END=1.00 PRIMER_PAIR_415_PRODUCT_SIZE=76 PRIMER_PAIR_416_PENALTY=6.571430 PRIMER_LEFT_416_PENALTY=2.660713 PRIMER_RIGHT_416_PENALTY=3.910717 PRIMER_LEFT_416_SEQUENCE=TGCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_416_SEQUENCE=GCACCAGTTCTGGATGGTCAC PRIMER_LEFT_416=416,22 PRIMER_RIGHT_416=493,21 PRIMER_LEFT_416_TM=60.661 PRIMER_RIGHT_416_TM=62.911 PRIMER_LEFT_416_GC_PERCENT=50.000 PRIMER_RIGHT_416_GC_PERCENT=57.143 PRIMER_LEFT_416_SELF_ANY=4.00 PRIMER_RIGHT_416_SELF_ANY=6.00 PRIMER_LEFT_416_SELF_END=0.00 PRIMER_RIGHT_416_SELF_END=3.00 PRIMER_LEFT_416_END_STABILITY=6.7000 PRIMER_RIGHT_416_END_STABILITY=6.1000 PRIMER_PAIR_416_COMPL_ANY=5.00 PRIMER_PAIR_416_COMPL_END=3.00 PRIMER_PAIR_416_PRODUCT_SIZE=78 PRIMER_PAIR_417_PENALTY=6.583729 PRIMER_LEFT_417_PENALTY=4.004895 PRIMER_RIGHT_417_PENALTY=2.578834 PRIMER_LEFT_417_SEQUENCE=AGGTACCCACTGATGGTAATGCT PRIMER_RIGHT_417_SEQUENCE=CACTTCCCATTCTGGACATT PRIMER_LEFT_417=255,23 PRIMER_RIGHT_417=354,20 PRIMER_LEFT_417_TM=61.005 PRIMER_RIGHT_417_TM=57.421 PRIMER_LEFT_417_GC_PERCENT=47.826 PRIMER_RIGHT_417_GC_PERCENT=45.000 PRIMER_LEFT_417_SELF_ANY=6.00 PRIMER_RIGHT_417_SELF_ANY=3.00 PRIMER_LEFT_417_SELF_END=0.00 PRIMER_RIGHT_417_SELF_END=1.00 PRIMER_LEFT_417_END_STABILITY=8.1000 PRIMER_RIGHT_417_END_STABILITY=6.6000 PRIMER_PAIR_417_COMPL_ANY=5.00 PRIMER_PAIR_417_COMPL_END=3.00 PRIMER_PAIR_417_PRODUCT_SIZE=100 PRIMER_PAIR_418_PENALTY=6.629471 PRIMER_LEFT_418_PENALTY=3.788644 PRIMER_RIGHT_418_PENALTY=2.840827 PRIMER_LEFT_418_SEQUENCE=CAACCAACCAGTGACCATC PRIMER_RIGHT_418_SEQUENCE=GCGGTAGGGAATCACAAAG PRIMER_LEFT_418=463,19 PRIMER_RIGHT_418=547,19 PRIMER_LEFT_418_TM=57.211 PRIMER_RIGHT_418_TM=58.159 PRIMER_LEFT_418_GC_PERCENT=52.632 PRIMER_RIGHT_418_GC_PERCENT=52.632 PRIMER_LEFT_418_SELF_ANY=3.00 PRIMER_RIGHT_418_SELF_ANY=3.00 PRIMER_LEFT_418_SELF_END=1.00 PRIMER_RIGHT_418_SELF_END=0.00 PRIMER_LEFT_418_END_STABILITY=8.1000 PRIMER_RIGHT_418_END_STABILITY=7.3000 PRIMER_PAIR_418_COMPL_ANY=4.00 PRIMER_PAIR_418_COMPL_END=1.00 PRIMER_PAIR_418_PRODUCT_SIZE=85 PRIMER_PAIR_419_PENALTY=6.645541 PRIMER_LEFT_419_PENALTY=2.419726 PRIMER_RIGHT_419_PENALTY=4.225815 PRIMER_LEFT_419_SEQUENCE=TTTGCGAAACTCATCTTCACTG PRIMER_RIGHT_419_SEQUENCE=CGTAGTCATGCAAGTTGGTACTCT PRIMER_LEFT_419=627,22 PRIMER_RIGHT_419=704,24 PRIMER_LEFT_419_TM=60.420 PRIMER_RIGHT_419_TM=59.774 PRIMER_LEFT_419_GC_PERCENT=40.909 PRIMER_RIGHT_419_GC_PERCENT=45.833 PRIMER_LEFT_419_SELF_ANY=4.00 PRIMER_RIGHT_419_SELF_ANY=5.00 PRIMER_LEFT_419_SELF_END=3.00 PRIMER_RIGHT_419_SELF_END=0.00 PRIMER_LEFT_419_END_STABILITY=6.7000 PRIMER_RIGHT_419_END_STABILITY=6.1000 PRIMER_PAIR_419_COMPL_ANY=4.00 PRIMER_PAIR_419_COMPL_END=2.00 PRIMER_PAIR_419_PRODUCT_SIZE=78 PRIMER_PAIR_420_PENALTY=6.664614 PRIMER_LEFT_420_PENALTY=4.457412 PRIMER_RIGHT_420_PENALTY=2.207202 PRIMER_LEFT_420_SEQUENCE=TGATGGTAATGCTGGCCT PRIMER_RIGHT_420_SEQUENCE=TCTGAATCCCACTTCCCATTC PRIMER_LEFT_420=265,18 PRIMER_RIGHT_420=363,21 PRIMER_LEFT_420_TM=57.543 PRIMER_RIGHT_420_TM=61.207 PRIMER_LEFT_420_GC_PERCENT=50.000 PRIMER_RIGHT_420_GC_PERCENT=47.619 PRIMER_LEFT_420_SELF_ANY=4.00 PRIMER_RIGHT_420_SELF_ANY=4.00 PRIMER_LEFT_420_SELF_END=2.00 PRIMER_RIGHT_420_SELF_END=2.00 PRIMER_LEFT_420_END_STABILITY=10.9000 PRIMER_RIGHT_420_END_STABILITY=6.9000 PRIMER_PAIR_420_COMPL_ANY=5.00 PRIMER_PAIR_420_COMPL_END=3.00 PRIMER_PAIR_420_PRODUCT_SIZE=99 PRIMER_PAIR_421_PENALTY=6.696336 PRIMER_LEFT_421_PENALTY=2.085443 PRIMER_RIGHT_421_PENALTY=4.610893 PRIMER_LEFT_421_SEQUENCE=CATCAGGGACCAAAACCTG PRIMER_RIGHT_421_SEQUENCE=GGCTTCTACCACATTGGTGATCT PRIMER_LEFT_421=366,19 PRIMER_RIGHT_421=463,23 PRIMER_LEFT_421_TM=58.915 PRIMER_RIGHT_421_TM=61.611 PRIMER_LEFT_421_GC_PERCENT=52.632 PRIMER_RIGHT_421_GC_PERCENT=47.826 PRIMER_LEFT_421_SELF_ANY=4.00 PRIMER_RIGHT_421_SELF_ANY=6.00 PRIMER_LEFT_421_SELF_END=1.00 PRIMER_RIGHT_421_SELF_END=2.00 PRIMER_LEFT_421_END_STABILITY=7.9000 PRIMER_RIGHT_421_END_STABILITY=6.3000 PRIMER_PAIR_421_COMPL_ANY=5.00 PRIMER_PAIR_421_COMPL_END=1.00 PRIMER_PAIR_421_PRODUCT_SIZE=98 PRIMER_PAIR_422_PENALTY=6.701185 PRIMER_LEFT_422_PENALTY=3.799463 PRIMER_RIGHT_422_PENALTY=2.901722 PRIMER_LEFT_422_SEQUENCE=AGATCACCAATGTGGTAGAAGC PRIMER_RIGHT_422_SEQUENCE=GGGGATGGGTCTTGCACT PRIMER_LEFT_422=441,22 PRIMER_RIGHT_422=527,18 PRIMER_LEFT_422_TM=58.201 PRIMER_RIGHT_422_TM=60.902 PRIMER_LEFT_422_GC_PERCENT=45.455 PRIMER_RIGHT_422_GC_PERCENT=61.111 PRIMER_LEFT_422_SELF_ANY=6.00 PRIMER_RIGHT_422_SELF_ANY=4.00 PRIMER_LEFT_422_SELF_END=2.00 PRIMER_RIGHT_422_SELF_END=1.00 PRIMER_LEFT_422_END_STABILITY=8.2000 PRIMER_RIGHT_422_END_STABILITY=7.9000 PRIMER_PAIR_422_COMPL_ANY=4.00 PRIMER_PAIR_422_COMPL_END=2.00 PRIMER_PAIR_422_PRODUCT_SIZE=87 PRIMER_PAIR_423_PENALTY=6.703381 PRIMER_LEFT_423_PENALTY=5.382554 PRIMER_RIGHT_423_PENALTY=1.320826 PRIMER_LEFT_423_SEQUENCE=AGACATGCAGTGAGAAGAGTACC PRIMER_RIGHT_423_SEQUENCE=AGTGGGCAACACACAAACTCT PRIMER_LEFT_423=666,23 PRIMER_RIGHT_423=765,21 PRIMER_LEFT_423_TM=57.617 PRIMER_RIGHT_423_TM=59.679 PRIMER_LEFT_423_GC_PERCENT=47.826 PRIMER_RIGHT_423_GC_PERCENT=47.619 PRIMER_LEFT_423_SELF_ANY=4.00 PRIMER_RIGHT_423_SELF_ANY=3.00 PRIMER_LEFT_423_SELF_END=2.00 PRIMER_RIGHT_423_SELF_END=1.00 PRIMER_LEFT_423_END_STABILITY=6.6000 PRIMER_RIGHT_423_END_STABILITY=6.1000 PRIMER_PAIR_423_COMPL_ANY=5.00 PRIMER_PAIR_423_COMPL_END=2.00 PRIMER_PAIR_423_PRODUCT_SIZE=100 PRIMER_PAIR_424_PENALTY=6.718133 PRIMER_LEFT_424_PENALTY=3.207117 PRIMER_RIGHT_424_PENALTY=3.511015 PRIMER_LEFT_424_SEQUENCE=AGTGATGCCCTTCTCGTTC PRIMER_RIGHT_424_SEQUENCE=GGTGTGCCAGTGAAGATGAGTT PRIMER_LEFT_424=569,19 PRIMER_RIGHT_424=655,22 PRIMER_LEFT_424_TM=57.793 PRIMER_RIGHT_424_TM=61.511 PRIMER_LEFT_424_GC_PERCENT=52.632 PRIMER_RIGHT_424_GC_PERCENT=50.000 PRIMER_LEFT_424_SELF_ANY=2.00 PRIMER_RIGHT_424_SELF_ANY=3.00 PRIMER_LEFT_424_SELF_END=0.00 PRIMER_RIGHT_424_SELF_END=0.00 PRIMER_LEFT_424_END_STABILITY=8.4000 PRIMER_RIGHT_424_END_STABILITY=6.4000 PRIMER_PAIR_424_COMPL_ANY=4.00 PRIMER_PAIR_424_COMPL_END=1.00 PRIMER_PAIR_424_PRODUCT_SIZE=87 PRIMER_PAIR_425_PENALTY=6.723436 PRIMER_LEFT_425_PENALTY=3.293963 PRIMER_RIGHT_425_PENALTY=3.429473 PRIMER_LEFT_425_SEQUENCE=GCTGCTTAGTTGGTGAGTTTG PRIMER_RIGHT_425_SEQUENCE=TTCGCAAACATCCATCCTCTC PRIMER_LEFT_425=546,21 PRIMER_RIGHT_425=634,21 PRIMER_LEFT_425_TM=57.706 PRIMER_RIGHT_425_TM=62.429 PRIMER_LEFT_425_GC_PERCENT=47.619 PRIMER_RIGHT_425_GC_PERCENT=47.619 PRIMER_LEFT_425_SELF_ANY=3.00 PRIMER_RIGHT_425_SELF_ANY=4.00 PRIMER_LEFT_425_SELF_END=0.00 PRIMER_RIGHT_425_SELF_END=0.00 PRIMER_LEFT_425_END_STABILITY=7.0000 PRIMER_RIGHT_425_END_STABILITY=6.4000 PRIMER_PAIR_425_COMPL_ANY=5.00 PRIMER_PAIR_425_COMPL_END=3.00 PRIMER_PAIR_425_PRODUCT_SIZE=89 PRIMER_PAIR_426_PENALTY=6.752968 PRIMER_LEFT_426_PENALTY=4.823759 PRIMER_RIGHT_426_PENALTY=1.929209 PRIMER_LEFT_426_SEQUENCE=GCCAAAGAGACATGCAGTGAGA PRIMER_RIGHT_426_SEQUENCE=TACCCCTCGGAACTTGTCA PRIMER_LEFT_426=659,22 PRIMER_RIGHT_426=745,19 PRIMER_LEFT_426_TM=62.824 PRIMER_RIGHT_426_TM=59.071 PRIMER_LEFT_426_GC_PERCENT=50.000 PRIMER_RIGHT_426_GC_PERCENT=52.632 PRIMER_LEFT_426_SELF_ANY=4.00 PRIMER_RIGHT_426_SELF_ANY=3.00 PRIMER_LEFT_426_SELF_END=0.00 PRIMER_RIGHT_426_SELF_END=3.00 PRIMER_LEFT_426_END_STABILITY=6.7000 PRIMER_RIGHT_426_END_STABILITY=6.7000 PRIMER_PAIR_426_COMPL_ANY=4.00 PRIMER_PAIR_426_COMPL_END=3.00 PRIMER_PAIR_426_PRODUCT_SIZE=87 PRIMER_PAIR_427_PENALTY=6.764893 PRIMER_LEFT_427_PENALTY=2.460554 PRIMER_RIGHT_427_PENALTY=4.304339 PRIMER_LEFT_427_SEQUENCE=TGCGAAACTCATCTTCACTG PRIMER_RIGHT_427_SEQUENCE=GCCGTAGTCATGCAAGTTGGTA PRIMER_LEFT_427=629,20 PRIMER_RIGHT_427=706,22 PRIMER_LEFT_427_TM=57.539 PRIMER_RIGHT_427_TM=62.304 PRIMER_LEFT_427_GC_PERCENT=45.000 PRIMER_RIGHT_427_GC_PERCENT=50.000 PRIMER_LEFT_427_SELF_ANY=3.00 PRIMER_RIGHT_427_SELF_ANY=5.00 PRIMER_LEFT_427_SELF_END=3.00 PRIMER_RIGHT_427_SELF_END=2.00 PRIMER_LEFT_427_END_STABILITY=6.7000 PRIMER_RIGHT_427_END_STABILITY=7.2000 PRIMER_PAIR_427_COMPL_ANY=4.00 PRIMER_PAIR_427_COMPL_END=3.00 PRIMER_PAIR_427_PRODUCT_SIZE=78 PRIMER_PAIR_428_PENALTY=6.779108 PRIMER_LEFT_428_PENALTY=2.538752 PRIMER_RIGHT_428_PENALTY=4.240356 PRIMER_LEFT_428_SEQUENCE=CTTACACCAGGAGAGGATGGAT PRIMER_RIGHT_428_SEQUENCE=CTTCTCACTGCATGTCTCTTTG PRIMER_LEFT_428=604,22 PRIMER_RIGHT_428=682,22 PRIMER_LEFT_428_TM=59.461 PRIMER_RIGHT_428_TM=57.760 PRIMER_LEFT_428_GC_PERCENT=50.000 PRIMER_RIGHT_428_GC_PERCENT=45.455 PRIMER_LEFT_428_SELF_ANY=3.00 PRIMER_RIGHT_428_SELF_ANY=4.00 PRIMER_LEFT_428_SELF_END=2.00 PRIMER_RIGHT_428_SELF_END=0.00 PRIMER_LEFT_428_END_STABILITY=8.1000 PRIMER_RIGHT_428_END_STABILITY=7.3000 PRIMER_PAIR_428_COMPL_ANY=5.00 PRIMER_PAIR_428_COMPL_END=3.00 PRIMER_PAIR_428_PRODUCT_SIZE=79 PRIMER_PAIR_429_PENALTY=6.780052 PRIMER_LEFT_429_PENALTY=3.878330 PRIMER_RIGHT_429_PENALTY=2.901722 PRIMER_LEFT_429_SEQUENCE=GATCACCAATGTGGTAGAAGC PRIMER_RIGHT_429_SEQUENCE=AGTGGGGATGGGTCTTGC PRIMER_LEFT_429=442,21 PRIMER_RIGHT_429=530,18 PRIMER_LEFT_429_TM=57.122 PRIMER_RIGHT_429_TM=60.902 PRIMER_LEFT_429_GC_PERCENT=47.619 PRIMER_RIGHT_429_GC_PERCENT=61.111 PRIMER_LEFT_429_SELF_ANY=6.00 PRIMER_RIGHT_429_SELF_ANY=2.00 PRIMER_LEFT_429_SELF_END=2.00 PRIMER_RIGHT_429_SELF_END=2.00 PRIMER_LEFT_429_END_STABILITY=8.2000 PRIMER_RIGHT_429_END_STABILITY=8.5000 PRIMER_PAIR_429_COMPL_ANY=5.00 PRIMER_PAIR_429_COMPL_END=2.00 PRIMER_PAIR_429_PRODUCT_SIZE=89 PRIMER_PAIR_430_PENALTY=6.817747 PRIMER_LEFT_430_PENALTY=3.107370 PRIMER_RIGHT_430_PENALTY=3.710377 PRIMER_LEFT_430_SEQUENCE=TGCAAATTCTTACACCAGGAG PRIMER_RIGHT_430_SEQUENCE=ACTGCATGTCTCTTTGGCGAC PRIMER_LEFT_430=596,21 PRIMER_RIGHT_430=676,21 PRIMER_LEFT_430_TM=57.893 PRIMER_RIGHT_430_TM=62.710 PRIMER_LEFT_430_GC_PERCENT=42.857 PRIMER_RIGHT_430_GC_PERCENT=52.381 PRIMER_LEFT_430_SELF_ANY=4.00 PRIMER_RIGHT_430_SELF_ANY=4.00 PRIMER_LEFT_430_SELF_END=3.00 PRIMER_RIGHT_430_SELF_END=2.00 PRIMER_LEFT_430_END_STABILITY=7.9000 PRIMER_RIGHT_430_END_STABILITY=9.6000 PRIMER_PAIR_430_COMPL_ANY=4.00 PRIMER_PAIR_430_COMPL_END=1.00 PRIMER_PAIR_430_PRODUCT_SIZE=81 PRIMER_PAIR_431_PENALTY=6.842086 PRIMER_LEFT_431_PENALTY=3.309654 PRIMER_RIGHT_431_PENALTY=3.532432 PRIMER_LEFT_431_SEQUENCE=CCCCACTTTGTGATTCCC PRIMER_RIGHT_431_SEQUENCE=AGAATTTGCACTTGTCAGGAA PRIMER_LEFT_431=524,18 PRIMER_RIGHT_431=605,21 PRIMER_LEFT_431_TM=58.690 PRIMER_RIGHT_431_TM=57.468 PRIMER_LEFT_431_GC_PERCENT=55.556 PRIMER_RIGHT_431_GC_PERCENT=38.095 PRIMER_LEFT_431_SELF_ANY=3.00 PRIMER_RIGHT_431_SELF_ANY=4.00 PRIMER_LEFT_431_SELF_END=3.00 PRIMER_RIGHT_431_SELF_END=0.00 PRIMER_LEFT_431_END_STABILITY=9.7000 PRIMER_RIGHT_431_END_STABILITY=8.2000 PRIMER_PAIR_431_COMPL_ANY=4.00 PRIMER_PAIR_431_COMPL_END=3.00 PRIMER_PAIR_431_PRODUCT_SIZE=82 PRIMER_PAIR_432_PENALTY=6.851355 PRIMER_LEFT_432_PENALTY=2.491155 PRIMER_RIGHT_432_PENALTY=4.360200 PRIMER_LEFT_432_SEQUENCE=GGATGTTTGCGAAACTCATCTT PRIMER_RIGHT_432_SEQUENCE=CATGCCGTAGTCATGCAA PRIMER_LEFT_432=622,22 PRIMER_RIGHT_432=709,18 PRIMER_LEFT_432_TM=60.491 PRIMER_RIGHT_432_TM=57.640 PRIMER_LEFT_432_GC_PERCENT=40.909 PRIMER_RIGHT_432_GC_PERCENT=50.000 PRIMER_LEFT_432_SELF_ANY=6.00 PRIMER_RIGHT_432_SELF_ANY=6.00 PRIMER_LEFT_432_SELF_END=2.00 PRIMER_RIGHT_432_SELF_END=2.00 PRIMER_LEFT_432_END_STABILITY=6.6000 PRIMER_RIGHT_432_END_STABILITY=8.8000 PRIMER_PAIR_432_COMPL_ANY=4.00 PRIMER_PAIR_432_COMPL_END=2.00 PRIMER_PAIR_432_PRODUCT_SIZE=88 PRIMER_PAIR_433_PENALTY=6.854565 PRIMER_LEFT_433_PENALTY=5.365271 PRIMER_RIGHT_433_PENALTY=1.489294 PRIMER_LEFT_433_SEQUENCE=AGAGACATGCAGTGAGAAGAGTACC PRIMER_RIGHT_433_SEQUENCE=GGCAACACACAAACTCTACCC PRIMER_LEFT_433=664,25 PRIMER_RIGHT_433=761,21 PRIMER_LEFT_433_TM=60.365 PRIMER_RIGHT_433_TM=59.511 PRIMER_LEFT_433_GC_PERCENT=48.000 PRIMER_RIGHT_433_GC_PERCENT=52.381 PRIMER_LEFT_433_SELF_ANY=4.00 PRIMER_RIGHT_433_SELF_ANY=2.00 PRIMER_LEFT_433_SELF_END=2.00 PRIMER_RIGHT_433_SELF_END=0.00 PRIMER_LEFT_433_END_STABILITY=6.6000 PRIMER_RIGHT_433_END_STABILITY=8.4000 PRIMER_PAIR_433_COMPL_ANY=5.00 PRIMER_PAIR_433_COMPL_END=2.00 PRIMER_PAIR_433_PRODUCT_SIZE=98 PRIMER_PAIR_434_PENALTY=6.873083 PRIMER_LEFT_434_PENALTY=3.791133 PRIMER_RIGHT_434_PENALTY=3.081950 PRIMER_LEFT_434_SEQUENCE=CAGTATTGCCAAGAAGTCTACCC PRIMER_RIGHT_434_SEQUENCE=TCTGGATGGTCACTGGTTG PRIMER_LEFT_434=410,23 PRIMER_RIGHT_434=485,19 PRIMER_LEFT_434_TM=59.209 PRIMER_RIGHT_434_TM=57.918 PRIMER_LEFT_434_GC_PERCENT=47.826 PRIMER_RIGHT_434_GC_PERCENT=52.632 PRIMER_LEFT_434_SELF_ANY=5.00 PRIMER_RIGHT_434_SELF_ANY=3.00 PRIMER_LEFT_434_SELF_END=0.00 PRIMER_RIGHT_434_SELF_END=0.00 PRIMER_LEFT_434_END_STABILITY=8.4000 PRIMER_RIGHT_434_END_STABILITY=8.2000 PRIMER_PAIR_434_COMPL_ANY=4.00 PRIMER_PAIR_434_COMPL_END=3.00 PRIMER_PAIR_434_PRODUCT_SIZE=76 PRIMER_PAIR_435_PENALTY=6.881481 PRIMER_LEFT_435_PENALTY=2.540392 PRIMER_RIGHT_435_PENALTY=4.341090 PRIMER_LEFT_435_SEQUENCE=TTACACCAGGAGAGGATGGAT PRIMER_RIGHT_435_SEQUENCE=CTTCTCACTGCATGTCTCTTTGG PRIMER_LEFT_435=605,21 PRIMER_RIGHT_435=682,23 PRIMER_LEFT_435_TM=58.460 PRIMER_RIGHT_435_TM=61.341 PRIMER_LEFT_435_GC_PERCENT=47.619 PRIMER_RIGHT_435_GC_PERCENT=47.826 PRIMER_LEFT_435_SELF_ANY=3.00 PRIMER_RIGHT_435_SELF_ANY=4.00 PRIMER_LEFT_435_SELF_END=2.00 PRIMER_RIGHT_435_SELF_END=0.00 PRIMER_LEFT_435_END_STABILITY=8.1000 PRIMER_RIGHT_435_END_STABILITY=8.8000 PRIMER_PAIR_435_COMPL_ANY=5.00 PRIMER_PAIR_435_COMPL_END=3.00 PRIMER_PAIR_435_PRODUCT_SIZE=78 PRIMER_PAIR_436_PENALTY=6.886599 PRIMER_LEFT_436_PENALTY=3.253441 PRIMER_RIGHT_436_PENALTY=3.633158 PRIMER_LEFT_436_SEQUENCE=ATGCCCTTCTCGTTCCTG PRIMER_RIGHT_436_SEQUENCE=GTGCCAGTGAAGATGAGTTTC PRIMER_LEFT_436=573,18 PRIMER_RIGHT_436=652,21 PRIMER_LEFT_436_TM=58.747 PRIMER_RIGHT_436_TM=57.367 PRIMER_LEFT_436_GC_PERCENT=55.556 PRIMER_RIGHT_436_GC_PERCENT=47.619 PRIMER_LEFT_436_SELF_ANY=2.00 PRIMER_RIGHT_436_SELF_ANY=3.00 PRIMER_LEFT_436_SELF_END=1.00 PRIMER_RIGHT_436_SELF_END=3.00 PRIMER_LEFT_436_END_STABILITY=8.2000 PRIMER_RIGHT_436_END_STABILITY=6.7000 PRIMER_PAIR_436_COMPL_ANY=4.00 PRIMER_PAIR_436_COMPL_END=1.00 PRIMER_PAIR_436_PRODUCT_SIZE=80 PRIMER_PAIR_437_PENALTY=6.898372 PRIMER_LEFT_437_PENALTY=3.117810 PRIMER_RIGHT_437_PENALTY=3.780562 PRIMER_LEFT_437_SEQUENCE=TTCCTGACAAGTGCAAATTCTT PRIMER_RIGHT_437_SEQUENCE=CATGTCTCTTTGGCGACGG PRIMER_LEFT_437=585,22 PRIMER_RIGHT_437=672,19 PRIMER_LEFT_437_TM=58.882 PRIMER_RIGHT_437_TM=62.781 PRIMER_LEFT_437_GC_PERCENT=36.364 PRIMER_RIGHT_437_GC_PERCENT=57.895 PRIMER_LEFT_437_SELF_ANY=4.00 PRIMER_RIGHT_437_SELF_ANY=4.00 PRIMER_LEFT_437_SELF_END=1.00 PRIMER_RIGHT_437_SELF_END=1.00 PRIMER_LEFT_437_END_STABILITY=7.0000 PRIMER_RIGHT_437_END_STABILITY=9.6000 PRIMER_PAIR_437_COMPL_ANY=4.00 PRIMER_PAIR_437_COMPL_END=0.00 PRIMER_PAIR_437_PRODUCT_SIZE=88 PRIMER_PAIR_438_PENALTY=6.918417 PRIMER_LEFT_438_PENALTY=3.320745 PRIMER_RIGHT_438_PENALTY=3.597672 PRIMER_LEFT_438_SEQUENCE=CTACCGCTGCTTAGTTGGTGAG PRIMER_RIGHT_438_SEQUENCE=AACATCCATCCTCTCCTGG PRIMER_LEFT_438=541,22 PRIMER_RIGHT_438=628,19 PRIMER_LEFT_438_TM=61.321 PRIMER_RIGHT_438_TM=57.402 PRIMER_LEFT_438_GC_PERCENT=54.545 PRIMER_RIGHT_438_GC_PERCENT=52.632 PRIMER_LEFT_438_SELF_ANY=3.00 PRIMER_RIGHT_438_SELF_ANY=3.00 PRIMER_LEFT_438_SELF_END=0.00 PRIMER_RIGHT_438_SELF_END=3.00 PRIMER_LEFT_438_END_STABILITY=6.4000 PRIMER_RIGHT_438_END_STABILITY=9.7000 PRIMER_PAIR_438_COMPL_ANY=4.00 PRIMER_PAIR_438_COMPL_END=1.00 PRIMER_PAIR_438_PRODUCT_SIZE=88 PRIMER_PAIR_439_PENALTY=6.979432 PRIMER_LEFT_439_PENALTY=2.186531 PRIMER_RIGHT_439_PENALTY=4.792901 PRIMER_LEFT_439_SEQUENCE=CAGGGACCAAAACCTGCATT PRIMER_RIGHT_439_SEQUENCE=CTTCTACCACATTGGTGATCTG PRIMER_LEFT_439=369,20 PRIMER_RIGHT_439=461,22 PRIMER_LEFT_439_TM=62.187 PRIMER_RIGHT_439_TM=57.207 PRIMER_LEFT_439_GC_PERCENT=50.000 PRIMER_RIGHT_439_GC_PERCENT=45.455 PRIMER_LEFT_439_SELF_ANY=4.00 PRIMER_RIGHT_439_SELF_ANY=6.00 PRIMER_LEFT_439_SELF_END=1.00 PRIMER_RIGHT_439_SELF_END=1.00 PRIMER_LEFT_439_END_STABILITY=8.4000 PRIMER_RIGHT_439_END_STABILITY=6.6000 PRIMER_PAIR_439_COMPL_ANY=5.00 PRIMER_PAIR_439_COMPL_END=0.00 PRIMER_PAIR_439_PRODUCT_SIZE=93 PRIMER_PAIR_440_PENALTY=6.992292 PRIMER_LEFT_440_PENALTY=3.738851 PRIMER_RIGHT_440_PENALTY=3.253441 PRIMER_LEFT_440_SEQUENCE=AGACCCATCCCCACTTTG PRIMER_RIGHT_440_SEQUENCE=CAGGAACGAGAAGGGCAT PRIMER_LEFT_440=516,18 PRIMER_RIGHT_440=590,18 PRIMER_LEFT_440_TM=58.261 PRIMER_RIGHT_440_TM=58.747 PRIMER_LEFT_440_GC_PERCENT=55.556 PRIMER_RIGHT_440_GC_PERCENT=55.556 PRIMER_LEFT_440_SELF_ANY=2.00 PRIMER_RIGHT_440_SELF_ANY=2.00 PRIMER_LEFT_440_SELF_END=1.00 PRIMER_RIGHT_440_SELF_END=2.00 PRIMER_LEFT_440_END_STABILITY=7.3000 PRIMER_RIGHT_440_END_STABILITY=9.6000 PRIMER_PAIR_440_COMPL_ANY=4.00 PRIMER_PAIR_440_COMPL_END=2.00 PRIMER_PAIR_440_PRODUCT_SIZE=75 PRIMER_PAIR_441_PENALTY=7.010512 PRIMER_LEFT_441_PENALTY=3.668956 PRIMER_RIGHT_441_PENALTY=3.341555 PRIMER_LEFT_441_SEQUENCE=GGGACCAAAACCTGCATT PRIMER_RIGHT_441_SEQUENCE=TTGGTTGGCTTCTACCACA PRIMER_LEFT_441=371,18 PRIMER_RIGHT_441=469,19 PRIMER_LEFT_441_TM=58.331 PRIMER_RIGHT_441_TM=57.658 PRIMER_LEFT_441_GC_PERCENT=50.000 PRIMER_RIGHT_441_GC_PERCENT=47.368 PRIMER_LEFT_441_SELF_ANY=4.00 PRIMER_RIGHT_441_SELF_ANY=4.00 PRIMER_LEFT_441_SELF_END=1.00 PRIMER_RIGHT_441_SELF_END=2.00 PRIMER_LEFT_441_END_STABILITY=8.4000 PRIMER_RIGHT_441_END_STABILITY=8.2000 PRIMER_PAIR_441_COMPL_ANY=5.00 PRIMER_PAIR_441_COMPL_END=1.00 PRIMER_PAIR_441_PRODUCT_SIZE=99 PRIMER_PAIR_442_PENALTY=7.020342 PRIMER_LEFT_442_PENALTY=4.566681 PRIMER_RIGHT_442_PENALTY=2.453661 PRIMER_LEFT_442_SEQUENCE=ACATGCACATGAATGTCCAGAAT PRIMER_RIGHT_442_SEQUENCE=CCTTGGTATCAATGCAGGTT PRIMER_LEFT_442=324,23 PRIMER_RIGHT_442=398,20 PRIMER_LEFT_442_TM=61.567 PRIMER_RIGHT_442_TM=57.546 PRIMER_LEFT_442_GC_PERCENT=39.130 PRIMER_RIGHT_442_GC_PERCENT=45.000 PRIMER_LEFT_442_SELF_ANY=8.00 PRIMER_RIGHT_442_SELF_ANY=6.00 PRIMER_LEFT_442_SELF_END=3.00 PRIMER_RIGHT_442_SELF_END=0.00 PRIMER_LEFT_442_END_STABILITY=6.6000 PRIMER_RIGHT_442_END_STABILITY=7.9000 PRIMER_PAIR_442_COMPL_ANY=5.00 PRIMER_PAIR_442_COMPL_END=1.00 PRIMER_PAIR_442_PRODUCT_SIZE=75 PRIMER_PAIR_443_PENALTY=7.029754 PRIMER_LEFT_443_PENALTY=3.681420 PRIMER_RIGHT_443_PENALTY=3.348334 PRIMER_LEFT_443_SEQUENCE=GAGGTACCCACTGATGGTAATG PRIMER_RIGHT_443_SEQUENCE=CATTCATGTGCATGTTCAGTCT PRIMER_LEFT_443=254,22 PRIMER_RIGHT_443=338,22 PRIMER_LEFT_443_TM=58.319 PRIMER_RIGHT_443_TM=58.652 PRIMER_LEFT_443_GC_PERCENT=50.000 PRIMER_RIGHT_443_GC_PERCENT=40.909 PRIMER_LEFT_443_SELF_ANY=6.00 PRIMER_RIGHT_443_SELF_ANY=6.00 PRIMER_LEFT_443_SELF_END=0.00 PRIMER_RIGHT_443_SELF_END=3.00 PRIMER_LEFT_443_END_STABILITY=6.2000 PRIMER_RIGHT_443_END_STABILITY=6.1000 PRIMER_PAIR_443_COMPL_ANY=5.00 PRIMER_PAIR_443_COMPL_END=1.00 PRIMER_PAIR_443_PRODUCT_SIZE=85 PRIMER_PAIR_444_PENALTY=7.038162 PRIMER_LEFT_444_PENALTY=1.628165 PRIMER_RIGHT_444_PENALTY=5.409997 PRIMER_LEFT_444_SEQUENCE=TCCAGAATGGGAAGTGGGAT PRIMER_RIGHT_444_SEQUENCE=AGGGTAGACTTCTTGGCAATACT PRIMER_LEFT_444=339,20 PRIMER_RIGHT_444=433,23 PRIMER_LEFT_444_TM=61.628 PRIMER_RIGHT_444_TM=57.590 PRIMER_LEFT_444_GC_PERCENT=50.000 PRIMER_RIGHT_444_GC_PERCENT=43.478 PRIMER_LEFT_444_SELF_ANY=3.00 PRIMER_RIGHT_444_SELF_ANY=5.00 PRIMER_LEFT_444_SELF_END=2.00 PRIMER_RIGHT_444_SELF_END=2.00 PRIMER_LEFT_444_END_STABILITY=9.3000 PRIMER_RIGHT_444_END_STABILITY=5.3000 PRIMER_PAIR_444_COMPL_ANY=5.00 PRIMER_PAIR_444_COMPL_END=1.00 PRIMER_PAIR_444_PRODUCT_SIZE=95 PRIMER_PAIR_445_PENALTY=7.046990 PRIMER_LEFT_445_PENALTY=2.151267 PRIMER_RIGHT_445_PENALTY=4.895724 PRIMER_LEFT_445_SEQUENCE=AGTGGGATTCAGATCCATCA PRIMER_RIGHT_445_SEQUENCE=ATCTGCAGTTCAGGGTAGACTT PRIMER_LEFT_445=351,20 PRIMER_RIGHT_445=444,22 PRIMER_LEFT_445_TM=57.849 PRIMER_RIGHT_445_TM=57.104 PRIMER_LEFT_445_GC_PERCENT=45.000 PRIMER_RIGHT_445_GC_PERCENT=45.455 PRIMER_LEFT_445_SELF_ANY=5.00 PRIMER_RIGHT_445_SELF_ANY=6.00 PRIMER_LEFT_445_SELF_END=2.00 PRIMER_RIGHT_445_SELF_END=1.00 PRIMER_LEFT_445_END_STABILITY=6.9000 PRIMER_RIGHT_445_END_STABILITY=6.4000 PRIMER_PAIR_445_COMPL_ANY=5.00 PRIMER_PAIR_445_COMPL_END=1.00 PRIMER_PAIR_445_PRODUCT_SIZE=94 PRIMER_PAIR_446_PENALTY=7.094532 PRIMER_LEFT_446_PENALTY=4.266053 PRIMER_RIGHT_446_PENALTY=2.828479 PRIMER_LEFT_446_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTA PRIMER_RIGHT_446_SEQUENCE=GGATGGTCACTGGTTGGTT PRIMER_LEFT_446=407,23 PRIMER_RIGHT_446=482,19 PRIMER_LEFT_446_TM=58.734 PRIMER_RIGHT_446_TM=58.172 PRIMER_LEFT_446_GC_PERCENT=43.478 PRIMER_RIGHT_446_GC_PERCENT=52.632 PRIMER_LEFT_446_SELF_ANY=6.00 PRIMER_RIGHT_446_SELF_ANY=3.00 PRIMER_LEFT_446_SELF_END=2.00 PRIMER_RIGHT_446_SELF_END=0.00 PRIMER_LEFT_446_END_STABILITY=5.4000 PRIMER_RIGHT_446_END_STABILITY=8.2000 PRIMER_PAIR_446_COMPL_ANY=5.00 PRIMER_PAIR_446_COMPL_END=1.00 PRIMER_PAIR_446_PRODUCT_SIZE=76 PRIMER_PAIR_447_PENALTY=7.095669 PRIMER_LEFT_447_PENALTY=3.409264 PRIMER_RIGHT_447_PENALTY=3.686405 PRIMER_LEFT_447_SEQUENCE=TCCCTACCGCTGCTTAGTT PRIMER_RIGHT_447_SEQUENCE=CCTGGTGTAAGAATTTGCACTT PRIMER_LEFT_447=538,19 PRIMER_RIGHT_447=614,22 PRIMER_LEFT_447_TM=57.591 PRIMER_RIGHT_447_TM=58.314 PRIMER_LEFT_447_GC_PERCENT=52.632 PRIMER_RIGHT_447_GC_PERCENT=40.909 PRIMER_LEFT_447_SELF_ANY=3.00 PRIMER_RIGHT_447_SELF_ANY=5.00 PRIMER_LEFT_447_SELF_END=0.00 PRIMER_RIGHT_447_SELF_END=2.00 PRIMER_LEFT_447_END_STABILITY=5.7000 PRIMER_RIGHT_447_END_STABILITY=6.7000 PRIMER_PAIR_447_COMPL_ANY=4.00 PRIMER_PAIR_447_COMPL_END=1.00 PRIMER_PAIR_447_PRODUCT_SIZE=77 PRIMER_PAIR_448_PENALTY=7.112405 PRIMER_LEFT_448_PENALTY=2.561455 PRIMER_RIGHT_448_PENALTY=4.550950 PRIMER_LEFT_448_SEQUENCE=TGCACATGAATGTCCAGAAT PRIMER_RIGHT_448_SEQUENCE=CTTCCTTGGTATCAATGCAGGTT PRIMER_LEFT_448=327,20 PRIMER_RIGHT_448=401,23 PRIMER_LEFT_448_TM=57.439 PRIMER_RIGHT_448_TM=61.551 PRIMER_LEFT_448_GC_PERCENT=40.000 PRIMER_RIGHT_448_GC_PERCENT=43.478 PRIMER_LEFT_448_SELF_ANY=8.00 PRIMER_RIGHT_448_SELF_ANY=8.00 PRIMER_LEFT_448_SELF_END=2.00 PRIMER_RIGHT_448_SELF_END=0.00 PRIMER_LEFT_448_END_STABILITY=6.6000 PRIMER_RIGHT_448_END_STABILITY=7.9000 PRIMER_PAIR_448_COMPL_ANY=4.00 PRIMER_PAIR_448_COMPL_END=2.00 PRIMER_PAIR_448_PRODUCT_SIZE=75 PRIMER_PAIR_449_PENALTY=7.127440 PRIMER_LEFT_449_PENALTY=3.409264 PRIMER_RIGHT_449_PENALTY=3.718176 PRIMER_LEFT_449_SEQUENCE=TTCCCTACCGCTGCTTAGT PRIMER_RIGHT_449_SEQUENCE=CCATCCTCTCCTGGTGTAAGAAT PRIMER_LEFT_449=537,19 PRIMER_RIGHT_449=623,23 PRIMER_LEFT_449_TM=57.591 PRIMER_RIGHT_449_TM=60.718 PRIMER_LEFT_449_GC_PERCENT=52.632 PRIMER_RIGHT_449_GC_PERCENT=47.826 PRIMER_LEFT_449_SELF_ANY=3.00 PRIMER_RIGHT_449_SELF_ANY=3.00 PRIMER_LEFT_449_SELF_END=1.00 PRIMER_RIGHT_449_SELF_END=2.00 PRIMER_LEFT_449_END_STABILITY=5.7000 PRIMER_RIGHT_449_END_STABILITY=6.6000 PRIMER_PAIR_449_COMPL_ANY=4.00 PRIMER_PAIR_449_COMPL_END=1.00 PRIMER_PAIR_449_PRODUCT_SIZE=87 PRIMER_PAIR_450_PENALTY=7.130001 PRIMER_LEFT_450_PENALTY=2.766033 PRIMER_RIGHT_450_PENALTY=4.363968 PRIMER_LEFT_450_SEQUENCE=TACACCAGGAGAGGATGGATGT PRIMER_RIGHT_450_SEQUENCE=TGGTACTCTTCTCACTGCATGTCT PRIMER_LEFT_450=606,22 PRIMER_RIGHT_450=689,24 PRIMER_LEFT_450_TM=60.766 PRIMER_RIGHT_450_TM=60.364 PRIMER_LEFT_450_GC_PERCENT=50.000 PRIMER_RIGHT_450_GC_PERCENT=45.833 PRIMER_LEFT_450_SELF_ANY=4.00 PRIMER_RIGHT_450_SELF_ANY=4.00 PRIMER_LEFT_450_SELF_END=0.00 PRIMER_RIGHT_450_SELF_END=0.00 PRIMER_LEFT_450_END_STABILITY=6.3000 PRIMER_RIGHT_450_END_STABILITY=6.4000 PRIMER_PAIR_450_COMPL_ANY=6.00 PRIMER_PAIR_450_COMPL_END=3.00 PRIMER_PAIR_450_PRODUCT_SIZE=84 PRIMER_PAIR_451_PENALTY=7.139908 PRIMER_LEFT_451_PENALTY=3.895774 PRIMER_RIGHT_451_PENALTY=3.244134 PRIMER_LEFT_451_SEQUENCE=CCAATGTGGTAGAAGCCAA PRIMER_RIGHT_451_SEQUENCE=AAGTGGGGATGGGTCTTGC PRIMER_LEFT_451=447,19 PRIMER_RIGHT_451=531,19 PRIMER_LEFT_451_TM=57.104 PRIMER_RIGHT_451_TM=62.244 PRIMER_LEFT_451_GC_PERCENT=47.368 PRIMER_RIGHT_451_GC_PERCENT=57.895 PRIMER_LEFT_451_SELF_ANY=4.00 PRIMER_RIGHT_451_SELF_ANY=3.00 PRIMER_LEFT_451_SELF_END=0.00 PRIMER_RIGHT_451_SELF_END=2.00 PRIMER_LEFT_451_END_STABILITY=10.0000 PRIMER_RIGHT_451_END_STABILITY=8.5000 PRIMER_PAIR_451_COMPL_ANY=3.00 PRIMER_PAIR_451_COMPL_END=2.00 PRIMER_PAIR_451_PRODUCT_SIZE=85 PRIMER_PAIR_452_PENALTY=7.154250 PRIMER_LEFT_452_PENALTY=3.791133 PRIMER_RIGHT_452_PENALTY=3.363116 PRIMER_LEFT_452_SEQUENCE=GTATTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_452_SEQUENCE=AGTTCTGGATGGTCACTGGTTG PRIMER_LEFT_452=412,23 PRIMER_RIGHT_452=488,22 PRIMER_LEFT_452_TM=59.209 PRIMER_RIGHT_452_TM=61.363 PRIMER_LEFT_452_GC_PERCENT=47.826 PRIMER_RIGHT_452_GC_PERCENT=50.000 PRIMER_LEFT_452_SELF_ANY=5.00 PRIMER_RIGHT_452_SELF_ANY=3.00 PRIMER_LEFT_452_SELF_END=1.00 PRIMER_RIGHT_452_SELF_END=0.00 PRIMER_LEFT_452_END_STABILITY=9.7000 PRIMER_RIGHT_452_END_STABILITY=8.2000 PRIMER_PAIR_452_COMPL_ANY=4.00 PRIMER_PAIR_452_COMPL_END=3.00 PRIMER_PAIR_452_PRODUCT_SIZE=77 PRIMER_PAIR_453_PENALTY=7.166911 PRIMER_LEFT_453_PENALTY=3.634478 PRIMER_RIGHT_453_PENALTY=3.532432 PRIMER_LEFT_453_SEQUENCE=ACCCATCCCCACTTTGTGATT PRIMER_RIGHT_453_SEQUENCE=AAGAATTTGCACTTGTCAGGA PRIMER_LEFT_453=518,21 PRIMER_RIGHT_453=606,21 PRIMER_LEFT_453_TM=62.634 PRIMER_RIGHT_453_TM=57.468 PRIMER_LEFT_453_GC_PERCENT=47.619 PRIMER_RIGHT_453_GC_PERCENT=38.095 PRIMER_LEFT_453_SELF_ANY=4.00 PRIMER_RIGHT_453_SELF_ANY=4.00 PRIMER_LEFT_453_SELF_END=2.00 PRIMER_RIGHT_453_SELF_END=2.00 PRIMER_LEFT_453_END_STABILITY=6.9000 PRIMER_RIGHT_453_END_STABILITY=8.2000 PRIMER_PAIR_453_COMPL_ANY=3.00 PRIMER_PAIR_453_COMPL_END=1.00 PRIMER_PAIR_453_PRODUCT_SIZE=89 PRIMER_PAIR_454_PENALTY=7.167486 PRIMER_LEFT_454_PENALTY=3.738851 PRIMER_RIGHT_454_PENALTY=3.428635 PRIMER_LEFT_454_SEQUENCE=CAAGACCCATCCCCACTT PRIMER_RIGHT_454_SEQUENCE=AGGAACGAGAAGGGCATCACT PRIMER_LEFT_454=514,18 PRIMER_RIGHT_454=589,21 PRIMER_LEFT_454_TM=58.261 PRIMER_RIGHT_454_TM=62.429 PRIMER_LEFT_454_GC_PERCENT=55.556 PRIMER_RIGHT_454_GC_PERCENT=52.381 PRIMER_LEFT_454_SELF_ANY=3.00 PRIMER_RIGHT_454_SELF_ANY=2.00 PRIMER_LEFT_454_SELF_END=0.00 PRIMER_RIGHT_454_SELF_END=2.00 PRIMER_LEFT_454_END_STABILITY=6.7000 PRIMER_RIGHT_454_END_STABILITY=6.4000 PRIMER_PAIR_454_COMPL_ANY=4.00 PRIMER_PAIR_454_COMPL_END=0.00 PRIMER_PAIR_454_PRODUCT_SIZE=76 PRIMER_PAIR_455_PENALTY=7.170029 PRIMER_LEFT_455_PENALTY=3.232712 PRIMER_RIGHT_455_PENALTY=3.937317 PRIMER_LEFT_455_SEQUENCE=GTGCAAATTCTTACACCAGGAG PRIMER_RIGHT_455_SEQUENCE=GCATGTCTCTTTGGCGACG PRIMER_LEFT_455=595,22 PRIMER_RIGHT_455=673,19 PRIMER_LEFT_455_TM=58.767 PRIMER_RIGHT_455_TM=62.937 PRIMER_LEFT_455_GC_PERCENT=45.455 PRIMER_RIGHT_455_GC_PERCENT=57.895 PRIMER_LEFT_455_SELF_ANY=5.00 PRIMER_RIGHT_455_SELF_ANY=4.00 PRIMER_LEFT_455_SELF_END=3.00 PRIMER_RIGHT_455_SELF_END=3.00 PRIMER_LEFT_455_END_STABILITY=7.9000 PRIMER_RIGHT_455_END_STABILITY=10.1000 PRIMER_PAIR_455_COMPL_ANY=4.00 PRIMER_PAIR_455_COMPL_END=2.00 PRIMER_PAIR_455_PRODUCT_SIZE=79 PRIMER_PAIR_456_PENALTY=7.207248 PRIMER_LEFT_456_PENALTY=4.366422 PRIMER_RIGHT_456_PENALTY=2.840827 PRIMER_LEFT_456_SEQUENCE=ATGTGGTAGAAGCCAACCAACC PRIMER_RIGHT_456_SEQUENCE=AGCGGTAGGGAATCACAAA PRIMER_LEFT_456=450,22 PRIMER_RIGHT_456=548,19 PRIMER_LEFT_456_TM=62.366 PRIMER_RIGHT_456_TM=58.159 PRIMER_LEFT_456_GC_PERCENT=50.000 PRIMER_RIGHT_456_GC_PERCENT=47.368 PRIMER_LEFT_456_SELF_ANY=4.00 PRIMER_RIGHT_456_SELF_ANY=3.00 PRIMER_LEFT_456_SELF_END=0.00 PRIMER_RIGHT_456_SELF_END=1.00 PRIMER_LEFT_456_END_STABILITY=8.2000 PRIMER_RIGHT_456_END_STABILITY=7.0000 PRIMER_PAIR_456_COMPL_ANY=4.00 PRIMER_PAIR_456_COMPL_END=0.00 PRIMER_PAIR_456_PRODUCT_SIZE=99 PRIMER_PAIR_457_PENALTY=7.239166 PRIMER_LEFT_457_PENALTY=4.076046 PRIMER_RIGHT_457_PENALTY=3.163121 PRIMER_LEFT_457_SEQUENCE=CAGTATTGCCAAGAAGTCTACCCT PRIMER_RIGHT_457_SEQUENCE=CTGGATGGTCACTGGTTGGTT PRIMER_LEFT_457=410,24 PRIMER_RIGHT_457=484,21 PRIMER_LEFT_457_TM=60.076 PRIMER_RIGHT_457_TM=62.163 PRIMER_LEFT_457_GC_PERCENT=45.833 PRIMER_RIGHT_457_GC_PERCENT=52.381 PRIMER_LEFT_457_SELF_ANY=5.00 PRIMER_RIGHT_457_SELF_ANY=3.00 PRIMER_LEFT_457_SELF_END=2.00 PRIMER_RIGHT_457_SELF_END=0.00 PRIMER_LEFT_457_END_STABILITY=9.1000 PRIMER_RIGHT_457_END_STABILITY=8.2000 PRIMER_PAIR_457_COMPL_ANY=5.00 PRIMER_PAIR_457_COMPL_END=3.00 PRIMER_PAIR_457_PRODUCT_SIZE=75 PRIMER_PAIR_458_PENALTY=7.264160 PRIMER_LEFT_458_PENALTY=2.788676 PRIMER_RIGHT_458_PENALTY=4.475484 PRIMER_LEFT_458_SEQUENCE=CCAGGAGAGGATGGATGTTTG PRIMER_RIGHT_458_SEQUENCE=TCATGCAAGTTGGTACTCTTCTC PRIMER_LEFT_458=610,21 PRIMER_RIGHT_458=699,23 PRIMER_LEFT_458_TM=61.789 PRIMER_RIGHT_458_TM=58.525 PRIMER_LEFT_458_GC_PERCENT=52.381 PRIMER_RIGHT_458_GC_PERCENT=43.478 PRIMER_LEFT_458_SELF_ANY=3.00 PRIMER_RIGHT_458_SELF_ANY=5.00 PRIMER_LEFT_458_SELF_END=0.00 PRIMER_RIGHT_458_SELF_END=0.00 PRIMER_LEFT_458_END_STABILITY=7.0000 PRIMER_RIGHT_458_END_STABILITY=6.7000 PRIMER_PAIR_458_COMPL_ANY=5.00 PRIMER_PAIR_458_COMPL_END=1.00 PRIMER_PAIR_458_PRODUCT_SIZE=90 PRIMER_PAIR_459_PENALTY=7.278031 PRIMER_LEFT_459_PENALTY=3.835187 PRIMER_RIGHT_459_PENALTY=3.442844 PRIMER_LEFT_459_SEQUENCE=GGAGGTACCCACTGATGGT PRIMER_RIGHT_459_SEQUENCE=GACATTCATGTGCATGTTCAGTC PRIMER_LEFT_459=253,19 PRIMER_RIGHT_459=340,23 PRIMER_LEFT_459_TM=57.165 PRIMER_RIGHT_459_TM=60.443 PRIMER_LEFT_459_GC_PERCENT=57.895 PRIMER_RIGHT_459_GC_PERCENT=43.478 PRIMER_LEFT_459_SELF_ANY=6.00 PRIMER_RIGHT_459_SELF_ANY=8.00 PRIMER_LEFT_459_SELF_END=3.00 PRIMER_RIGHT_459_SELF_END=2.00 PRIMER_LEFT_459_END_STABILITY=7.8000 PRIMER_RIGHT_459_END_STABILITY=6.4000 PRIMER_PAIR_459_COMPL_ANY=5.00 PRIMER_PAIR_459_COMPL_END=2.00 PRIMER_PAIR_459_PRODUCT_SIZE=88 PRIMER_PAIR_460_PENALTY=7.321073 PRIMER_LEFT_460_PENALTY=3.428635 PRIMER_RIGHT_460_PENALTY=3.892438 PRIMER_LEFT_460_SEQUENCE=AGTGATGCCCTTCTCGTTCCT PRIMER_RIGHT_460_SEQUENCE=CGGTGTGCCAGTGAAGAT PRIMER_LEFT_460=569,21 PRIMER_RIGHT_460=656,18 PRIMER_LEFT_460_TM=62.429 PRIMER_RIGHT_460_TM=58.108 PRIMER_LEFT_460_GC_PERCENT=52.381 PRIMER_RIGHT_460_GC_PERCENT=55.556 PRIMER_LEFT_460_SELF_ANY=2.00 PRIMER_RIGHT_460_SELF_ANY=3.00 PRIMER_LEFT_460_SELF_END=0.00 PRIMER_RIGHT_460_SELF_END=2.00 PRIMER_LEFT_460_END_STABILITY=8.2000 PRIMER_RIGHT_460_END_STABILITY=6.6000 PRIMER_PAIR_460_COMPL_ANY=4.00 PRIMER_PAIR_460_COMPL_END=2.00 PRIMER_PAIR_460_PRODUCT_SIZE=88 PRIMER_PAIR_461_PENALTY=7.353529 PRIMER_LEFT_461_PENALTY=4.457023 PRIMER_RIGHT_461_PENALTY=2.896506 PRIMER_LEFT_461_SEQUENCE=AAGCCAACCAACCAGTGA PRIMER_RIGHT_461_SEQUENCE=CAGCGGTAGGGAATCACAAAG PRIMER_LEFT_461=459,18 PRIMER_RIGHT_461=549,21 PRIMER_LEFT_461_TM=57.543 PRIMER_RIGHT_461_TM=61.897 PRIMER_LEFT_461_GC_PERCENT=50.000 PRIMER_RIGHT_461_GC_PERCENT=52.381 PRIMER_LEFT_461_SELF_ANY=3.00 PRIMER_RIGHT_461_SELF_ANY=3.00 PRIMER_LEFT_461_SELF_END=1.00 PRIMER_RIGHT_461_SELF_END=0.00 PRIMER_LEFT_461_END_STABILITY=6.4000 PRIMER_RIGHT_461_END_STABILITY=7.3000 PRIMER_PAIR_461_COMPL_ANY=4.00 PRIMER_PAIR_461_COMPL_END=1.00 PRIMER_PAIR_461_PRODUCT_SIZE=91 PRIMER_PAIR_462_PENALTY=7.355960 PRIMER_LEFT_462_PENALTY=3.776506 PRIMER_RIGHT_462_PENALTY=3.579454 PRIMER_LEFT_462_SEQUENCE=ATCCCCACTTTGTGATTCCCTA PRIMER_RIGHT_462_SEQUENCE=ATTTGCACTTGTCAGGAACGAG PRIMER_LEFT_462=522,22 PRIMER_RIGHT_462=602,22 PRIMER_LEFT_462_TM=61.777 PRIMER_RIGHT_462_TM=61.579 PRIMER_LEFT_462_GC_PERCENT=45.455 PRIMER_RIGHT_462_GC_PERCENT=45.455 PRIMER_LEFT_462_SELF_ANY=4.00 PRIMER_RIGHT_462_SELF_ANY=4.00 PRIMER_LEFT_462_SELF_END=3.00 PRIMER_RIGHT_462_SELF_END=2.00 PRIMER_LEFT_462_END_STABILITY=8.7000 PRIMER_RIGHT_462_END_STABILITY=8.1000 PRIMER_PAIR_462_COMPL_ANY=4.00 PRIMER_PAIR_462_COMPL_END=1.00 PRIMER_PAIR_462_PRODUCT_SIZE=81 PRIMER_PAIR_463_PENALTY=7.372506 PRIMER_LEFT_463_PENALTY=2.833316 PRIMER_RIGHT_463_PENALTY=4.539189 PRIMER_LEFT_463_SEQUENCE=CTTACACCAGGAGAGGATGG PRIMER_RIGHT_463_SEQUENCE=GGTACTCTTCTCACTGCATGTCTC PRIMER_LEFT_463=604,20 PRIMER_RIGHT_463=688,24 PRIMER_LEFT_463_TM=57.167 PRIMER_RIGHT_463_TM=59.461 PRIMER_LEFT_463_GC_PERCENT=55.000 PRIMER_RIGHT_463_GC_PERCENT=50.000 PRIMER_LEFT_463_SELF_ANY=3.00 PRIMER_RIGHT_463_SELF_ANY=4.00 PRIMER_LEFT_463_SELF_END=0.00 PRIMER_RIGHT_463_SELF_END=0.00 PRIMER_LEFT_463_END_STABILITY=8.1000 PRIMER_RIGHT_463_END_STABILITY=6.1000 PRIMER_PAIR_463_COMPL_ANY=6.00 PRIMER_PAIR_463_COMPL_END=3.00 PRIMER_PAIR_463_PRODUCT_SIZE=85 PRIMER_PAIR_464_PENALTY=7.381861 PRIMER_LEFT_464_PENALTY=3.413777 PRIMER_RIGHT_464_PENALTY=3.968084 PRIMER_LEFT_464_SEQUENCE=GCTGCTTAGTTGGTGAGTTTGT PRIMER_RIGHT_464_SEQUENCE=TCGCAAACATCCATCCTC PRIMER_LEFT_464=546,22 PRIMER_RIGHT_464=633,18 PRIMER_LEFT_464_TM=58.586 PRIMER_RIGHT_464_TM=58.032 PRIMER_LEFT_464_GC_PERCENT=45.455 PRIMER_RIGHT_464_GC_PERCENT=50.000 PRIMER_LEFT_464_SELF_ANY=3.00 PRIMER_RIGHT_464_SELF_ANY=2.00 PRIMER_LEFT_464_SELF_END=0.00 PRIMER_RIGHT_464_SELF_END=0.00 PRIMER_LEFT_464_END_STABILITY=7.0000 PRIMER_RIGHT_464_END_STABILITY=7.9000 PRIMER_PAIR_464_COMPL_ANY=5.00 PRIMER_PAIR_464_COMPL_END=2.00 PRIMER_PAIR_464_PRODUCT_SIZE=88 PRIMER_PAIR_465_PENALTY=7.404682 PRIMER_LEFT_465_PENALTY=3.823109 PRIMER_RIGHT_465_PENALTY=3.581573 PRIMER_LEFT_465_SEQUENCE=ATCCCCACTTTGTGATTCC PRIMER_RIGHT_465_SEQUENCE=GCACTTGTCAGGAACGAGA PRIMER_LEFT_465=522,19 PRIMER_RIGHT_465=598,19 PRIMER_LEFT_465_TM=57.177 PRIMER_RIGHT_465_TM=57.418 PRIMER_LEFT_465_GC_PERCENT=47.368 PRIMER_RIGHT_465_GC_PERCENT=52.632 PRIMER_LEFT_465_SELF_ANY=4.00 PRIMER_RIGHT_465_SELF_ANY=3.00 PRIMER_LEFT_465_SELF_END=1.00 PRIMER_RIGHT_465_SELF_END=0.00 PRIMER_LEFT_465_END_STABILITY=8.1000 PRIMER_RIGHT_465_END_STABILITY=8.4000 PRIMER_PAIR_465_COMPL_ANY=4.00 PRIMER_PAIR_465_COMPL_END=3.00 PRIMER_PAIR_465_PRODUCT_SIZE=77 PRIMER_PAIR_466_PENALTY=7.411188 PRIMER_LEFT_466_PENALTY=2.643243 PRIMER_RIGHT_466_PENALTY=4.767944 PRIMER_LEFT_466_SEQUENCE=AAACTCATCTTCACTGGCACA PRIMER_RIGHT_466_SEQUENCE=GTCAATTCCGCAGGGCAG PRIMER_LEFT_466=633,21 PRIMER_RIGHT_466=730,18 PRIMER_LEFT_466_TM=58.357 PRIMER_RIGHT_466_TM=62.768 PRIMER_LEFT_466_GC_PERCENT=42.857 PRIMER_RIGHT_466_GC_PERCENT=61.111 PRIMER_LEFT_466_SELF_ANY=3.00 PRIMER_RIGHT_466_SELF_ANY=4.00 PRIMER_LEFT_466_SELF_END=2.00 PRIMER_RIGHT_466_SELF_END=1.00 PRIMER_LEFT_466_END_STABILITY=8.2000 PRIMER_RIGHT_466_END_STABILITY=9.7000 PRIMER_PAIR_466_COMPL_ANY=4.00 PRIMER_PAIR_466_COMPL_END=2.00 PRIMER_PAIR_466_PRODUCT_SIZE=98 PRIMER_PAIR_467_PENALTY=7.416007 PRIMER_LEFT_467_PENALTY=3.232712 PRIMER_RIGHT_467_PENALTY=4.183295 PRIMER_LEFT_467_SEQUENCE=AGTGCAAATTCTTACACCAGGA PRIMER_RIGHT_467_SEQUENCE=GCATGTCTCTTTGGCGAC PRIMER_LEFT_467=594,22 PRIMER_RIGHT_467=673,18 PRIMER_LEFT_467_TM=58.767 PRIMER_RIGHT_467_TM=57.817 PRIMER_LEFT_467_GC_PERCENT=40.909 PRIMER_RIGHT_467_GC_PERCENT=55.556 PRIMER_LEFT_467_SELF_ANY=5.00 PRIMER_RIGHT_467_SELF_ANY=4.00 PRIMER_LEFT_467_SELF_END=1.00 PRIMER_RIGHT_467_SELF_END=2.00 PRIMER_LEFT_467_END_STABILITY=8.2000 PRIMER_RIGHT_467_END_STABILITY=9.6000 PRIMER_PAIR_467_COMPL_ANY=4.00 PRIMER_PAIR_467_COMPL_END=0.00 PRIMER_PAIR_467_PRODUCT_SIZE=80 PRIMER_PAIR_468_PENALTY=7.439162 PRIMER_LEFT_468_PENALTY=4.076046 PRIMER_RIGHT_468_PENALTY=3.363116 PRIMER_LEFT_468_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_468_SEQUENCE=CAGTTCTGGATGGTCACTGGTT PRIMER_LEFT_468=411,24 PRIMER_RIGHT_468=489,22 PRIMER_LEFT_468_TM=60.076 PRIMER_RIGHT_468_TM=61.363 PRIMER_LEFT_468_GC_PERCENT=45.833 PRIMER_RIGHT_468_GC_PERCENT=50.000 PRIMER_LEFT_468_SELF_ANY=5.00 PRIMER_RIGHT_468_SELF_ANY=4.00 PRIMER_LEFT_468_SELF_END=1.00 PRIMER_RIGHT_468_SELF_END=0.00 PRIMER_LEFT_468_END_STABILITY=9.7000 PRIMER_RIGHT_468_END_STABILITY=8.2000 PRIMER_PAIR_468_COMPL_ANY=4.00 PRIMER_PAIR_468_COMPL_END=2.00 PRIMER_PAIR_468_PRODUCT_SIZE=79 PRIMER_PAIR_469_PENALTY=7.454993 PRIMER_LEFT_469_PENALTY=4.397076 PRIMER_RIGHT_469_PENALTY=3.057917 PRIMER_LEFT_469_SEQUENCE=AGAACTGGTGCAAGCGGG PRIMER_RIGHT_469_SEQUENCE=CACCAACTAAGCAGCGGTAGG PRIMER_LEFT_469=483,18 PRIMER_RIGHT_469=560,21 PRIMER_LEFT_469_TM=62.397 PRIMER_RIGHT_469_TM=62.058 PRIMER_LEFT_469_GC_PERCENT=61.111 PRIMER_RIGHT_469_GC_PERCENT=57.143 PRIMER_LEFT_469_SELF_ANY=4.00 PRIMER_RIGHT_469_SELF_ANY=3.00 PRIMER_LEFT_469_SELF_END=0.00 PRIMER_RIGHT_469_SELF_END=1.00 PRIMER_LEFT_469_END_STABILITY=12.9000 PRIMER_RIGHT_469_END_STABILITY=6.9000 PRIMER_PAIR_469_COMPL_ANY=5.00 PRIMER_PAIR_469_COMPL_END=0.00 PRIMER_PAIR_469_PRODUCT_SIZE=78 PRIMER_PAIR_470_PENALTY=7.459067 PRIMER_LEFT_470_PENALTY=4.149413 PRIMER_RIGHT_470_PENALTY=3.309654 PRIMER_LEFT_470_SEQUENCE=CAATGTGGTAGAAGCCAACCAA PRIMER_RIGHT_470_SEQUENCE=GGGAATCACAAAGTGGGG PRIMER_LEFT_470=448,22 PRIMER_RIGHT_470=541,18 PRIMER_LEFT_470_TM=62.149 PRIMER_RIGHT_470_TM=58.690 PRIMER_LEFT_470_GC_PERCENT=45.455 PRIMER_RIGHT_470_GC_PERCENT=55.556 PRIMER_LEFT_470_SELF_ANY=4.00 PRIMER_RIGHT_470_SELF_ANY=3.00 PRIMER_LEFT_470_SELF_END=2.00 PRIMER_RIGHT_470_SELF_END=0.00 PRIMER_LEFT_470_END_STABILITY=8.2000 PRIMER_RIGHT_470_END_STABILITY=11.2000 PRIMER_PAIR_470_COMPL_ANY=4.00 PRIMER_PAIR_470_COMPL_END=1.00 PRIMER_PAIR_470_PRODUCT_SIZE=94 PRIMER_PAIR_471_PENALTY=7.468686 PRIMER_LEFT_471_PENALTY=3.477475 PRIMER_RIGHT_471_PENALTY=3.991211 PRIMER_LEFT_471_SEQUENCE=TACCGCTGCTTAGTTGGTG PRIMER_RIGHT_471_SEQUENCE=CTCTCCTGGTGTAAGAATTTGC PRIMER_LEFT_471=542,19 PRIMER_RIGHT_471=618,22 PRIMER_LEFT_471_TM=57.523 PRIMER_RIGHT_471_TM=58.009 PRIMER_LEFT_471_GC_PERCENT=52.632 PRIMER_RIGHT_471_GC_PERCENT=45.455 PRIMER_LEFT_471_SELF_ANY=3.00 PRIMER_RIGHT_471_SELF_ANY=4.00 PRIMER_LEFT_471_SELF_END=0.00 PRIMER_RIGHT_471_SELF_END=2.00 PRIMER_LEFT_471_END_STABILITY=8.2000 PRIMER_RIGHT_471_END_STABILITY=8.8000 PRIMER_PAIR_471_COMPL_ANY=4.00 PRIMER_PAIR_471_COMPL_END=1.00 PRIMER_PAIR_471_PRODUCT_SIZE=77 PRIMER_PAIR_472_PENALTY=7.474878 PRIMER_LEFT_472_PENALTY=3.077802 PRIMER_RIGHT_472_PENALTY=4.397076 PRIMER_LEFT_472_SEQUENCE=GCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_472_SEQUENCE=CCCGCTTGCACCAGTTCT PRIMER_LEFT_472=417,21 PRIMER_RIGHT_472=500,18 PRIMER_LEFT_472_TM=57.922 PRIMER_RIGHT_472_TM=62.397 PRIMER_LEFT_472_GC_PERCENT=52.381 PRIMER_RIGHT_472_GC_PERCENT=61.111 PRIMER_LEFT_472_SELF_ANY=4.00 PRIMER_RIGHT_472_SELF_ANY=4.00 PRIMER_LEFT_472_SELF_END=0.00 PRIMER_RIGHT_472_SELF_END=2.00 PRIMER_LEFT_472_END_STABILITY=6.7000 PRIMER_RIGHT_472_END_STABILITY=6.4000 PRIMER_PAIR_472_COMPL_ANY=4.00 PRIMER_PAIR_472_COMPL_END=3.00 PRIMER_PAIR_472_PRODUCT_SIZE=84 PRIMER_PAIR_473_PENALTY=7.512983 PRIMER_LEFT_473_PENALTY=5.114578 PRIMER_RIGHT_473_PENALTY=2.398404 PRIMER_LEFT_473_SEQUENCE=AAAGAGACATGCAGTGAGAAGAG PRIMER_RIGHT_473_SEQUENCE=CACAAACTCTACCCCTCGGAA PRIMER_LEFT_473=662,23 PRIMER_RIGHT_473=754,21 PRIMER_LEFT_473_TM=57.885 PRIMER_RIGHT_473_TM=61.398 PRIMER_LEFT_473_GC_PERCENT=43.478 PRIMER_RIGHT_473_GC_PERCENT=52.381 PRIMER_LEFT_473_SELF_ANY=4.00 PRIMER_RIGHT_473_SELF_ANY=3.00 PRIMER_LEFT_473_SELF_END=0.00 PRIMER_RIGHT_473_SELF_END=1.00 PRIMER_LEFT_473_END_STABILITY=6.7000 PRIMER_RIGHT_473_END_STABILITY=10.2000 PRIMER_PAIR_473_COMPL_ANY=4.00 PRIMER_PAIR_473_COMPL_END=2.00 PRIMER_PAIR_473_PRODUCT_SIZE=93 PRIMER_PAIR_474_PENALTY=7.527837 PRIMER_LEFT_474_PENALTY=4.457023 PRIMER_RIGHT_474_PENALTY=3.070814 PRIMER_LEFT_474_SEQUENCE=GAAGCCAACCAACCAGTG PRIMER_RIGHT_474_SEQUENCE=TAAGCAGCGGTAGGGAATC PRIMER_LEFT_474=458,18 PRIMER_RIGHT_474=553,19 PRIMER_LEFT_474_TM=57.543 PRIMER_RIGHT_474_TM=57.929 PRIMER_LEFT_474_GC_PERCENT=55.556 PRIMER_RIGHT_474_GC_PERCENT=52.632 PRIMER_LEFT_474_SELF_ANY=3.00 PRIMER_RIGHT_474_SELF_ANY=3.00 PRIMER_LEFT_474_SELF_END=3.00 PRIMER_RIGHT_474_SELF_END=3.00 PRIMER_LEFT_474_END_STABILITY=6.7000 PRIMER_RIGHT_474_END_STABILITY=6.6000 PRIMER_PAIR_474_COMPL_ANY=4.00 PRIMER_PAIR_474_COMPL_END=1.00 PRIMER_PAIR_474_PRODUCT_SIZE=96 PRIMER_PAIR_475_PENALTY=7.531194 PRIMER_LEFT_475_PENALTY=2.880239 PRIMER_RIGHT_475_PENALTY=4.650955 PRIMER_LEFT_475_SEQUENCE=GGAGAGGATGGATGTTTGCG PRIMER_RIGHT_475_SEQUENCE=GTCATGCAAGTTGGTACTCTTCTC PRIMER_LEFT_475=613,20 PRIMER_RIGHT_475=700,24 PRIMER_LEFT_475_TM=62.880 PRIMER_RIGHT_475_TM=59.349 PRIMER_LEFT_475_GC_PERCENT=55.000 PRIMER_RIGHT_475_GC_PERCENT=45.833 PRIMER_LEFT_475_SELF_ANY=2.00 PRIMER_RIGHT_475_SELF_ANY=5.00 PRIMER_LEFT_475_SELF_END=2.00 PRIMER_RIGHT_475_SELF_END=0.00 PRIMER_LEFT_475_END_STABILITY=10.5000 PRIMER_RIGHT_475_END_STABILITY=6.7000 PRIMER_PAIR_475_COMPL_ANY=5.00 PRIMER_PAIR_475_COMPL_END=1.00 PRIMER_PAIR_475_PRODUCT_SIZE=88 PRIMER_PAIR_476_PENALTY=7.533857 PRIMER_LEFT_476_PENALTY=3.904052 PRIMER_RIGHT_476_PENALTY=3.629805 PRIMER_LEFT_476_SEQUENCE=GACCCATCCCCACTTTGT PRIMER_RIGHT_476_SEQUENCE=AGAATTTGCACTTGTCAGGAAC PRIMER_LEFT_476=517,18 PRIMER_RIGHT_476=605,22 PRIMER_LEFT_476_TM=58.096 PRIMER_RIGHT_476_TM=58.370 PRIMER_LEFT_476_GC_PERCENT=55.556 PRIMER_RIGHT_476_GC_PERCENT=40.909 PRIMER_LEFT_476_SELF_ANY=2.00 PRIMER_RIGHT_476_SELF_ANY=4.00 PRIMER_LEFT_476_SELF_END=1.00 PRIMER_RIGHT_476_SELF_END=1.00 PRIMER_LEFT_476_END_STABILITY=7.0000 PRIMER_RIGHT_476_END_STABILITY=7.9000 PRIMER_PAIR_476_COMPL_ANY=3.00 PRIMER_PAIR_476_COMPL_END=2.00 PRIMER_PAIR_476_PRODUCT_SIZE=89 PRIMER_PAIR_477_PENALTY=7.538089 PRIMER_LEFT_477_PENALTY=3.486268 PRIMER_RIGHT_477_PENALTY=4.051820 PRIMER_LEFT_477_SEQUENCE=CCGCTGCTTAGTTGGTGA PRIMER_RIGHT_477_SEQUENCE=TTTCGCAAACATCCATCC PRIMER_LEFT_477=544,18 PRIMER_RIGHT_477=635,18 PRIMER_LEFT_477_TM=58.514 PRIMER_RIGHT_477_TM=57.948 PRIMER_LEFT_477_GC_PERCENT=55.556 PRIMER_RIGHT_477_GC_PERCENT=44.444 PRIMER_LEFT_477_SELF_ANY=3.00 PRIMER_RIGHT_477_SELF_ANY=4.00 PRIMER_LEFT_477_SELF_END=1.00 PRIMER_RIGHT_477_SELF_END=0.00 PRIMER_LEFT_477_END_STABILITY=7.9000 PRIMER_RIGHT_477_END_STABILITY=8.1000 PRIMER_PAIR_477_COMPL_ANY=4.00 PRIMER_PAIR_477_COMPL_END=2.00 PRIMER_PAIR_477_PRODUCT_SIZE=92 PRIMER_PAIR_478_PENALTY=7.552575 PRIMER_LEFT_478_PENALTY=3.918097 PRIMER_RIGHT_478_PENALTY=3.634478 PRIMER_LEFT_478_SEQUENCE=CAGATCACCAATGTGGTAGAAGC PRIMER_RIGHT_478_SEQUENCE=AATCACAAAGTGGGGATGGGT PRIMER_LEFT_478=440,23 PRIMER_RIGHT_478=538,21 PRIMER_LEFT_478_TM=60.918 PRIMER_RIGHT_478_TM=62.634 PRIMER_LEFT_478_GC_PERCENT=47.826 PRIMER_RIGHT_478_GC_PERCENT=47.619 PRIMER_LEFT_478_SELF_ANY=6.00 PRIMER_RIGHT_478_SELF_ANY=4.00 PRIMER_LEFT_478_SELF_END=2.00 PRIMER_RIGHT_478_SELF_END=0.00 PRIMER_LEFT_478_END_STABILITY=8.2000 PRIMER_RIGHT_478_END_STABILITY=9.4000 PRIMER_PAIR_478_COMPL_ANY=6.00 PRIMER_PAIR_478_COMPL_END=1.00 PRIMER_PAIR_478_PRODUCT_SIZE=99 PRIMER_PAIR_479_PENALTY=7.552674 PRIMER_LEFT_479_PENALTY=3.874309 PRIMER_RIGHT_479_PENALTY=3.678365 PRIMER_LEFT_479_SEQUENCE=TGTGATTCCCTACCGCTG PRIMER_RIGHT_479_SEQUENCE=TGTAAGAATTTGCACTTGTCAGG PRIMER_LEFT_479=532,18 PRIMER_RIGHT_479=609,23 PRIMER_LEFT_479_TM=58.126 PRIMER_RIGHT_479_TM=59.322 PRIMER_LEFT_479_GC_PERCENT=55.556 PRIMER_RIGHT_479_GC_PERCENT=39.130 PRIMER_LEFT_479_SELF_ANY=3.00 PRIMER_RIGHT_479_SELF_ANY=4.00 PRIMER_LEFT_479_SELF_END=2.00 PRIMER_RIGHT_479_SELF_END=3.00 PRIMER_LEFT_479_END_STABILITY=10.2000 PRIMER_RIGHT_479_END_STABILITY=8.2000 PRIMER_PAIR_479_COMPL_ANY=4.00 PRIMER_PAIR_479_COMPL_END=2.00 PRIMER_PAIR_479_PRODUCT_SIZE=78 PRIMER_PAIR_480_PENALTY=7.596215 PRIMER_LEFT_480_PENALTY=3.495754 PRIMER_RIGHT_480_PENALTY=4.100461 PRIMER_LEFT_480_SEQUENCE=TTTGTAAGTGATGCCCTTCTCG PRIMER_RIGHT_480_SEQUENCE=GTGTGCCAGTGAAGATGAGTTTC PRIMER_LEFT_480=563,22 PRIMER_RIGHT_480=654,23 PRIMER_LEFT_480_TM=61.496 PRIMER_RIGHT_480_TM=61.100 PRIMER_LEFT_480_GC_PERCENT=45.455 PRIMER_RIGHT_480_GC_PERCENT=47.826 PRIMER_LEFT_480_SELF_ANY=4.00 PRIMER_RIGHT_480_SELF_ANY=3.00 PRIMER_LEFT_480_SELF_END=2.00 PRIMER_RIGHT_480_SELF_END=3.00 PRIMER_LEFT_480_END_STABILITY=8.4000 PRIMER_RIGHT_480_END_STABILITY=6.7000 PRIMER_PAIR_480_COMPL_ANY=4.00 PRIMER_PAIR_480_COMPL_END=1.00 PRIMER_PAIR_480_PRODUCT_SIZE=92 PRIMER_PAIR_481_PENALTY=7.599974 PRIMER_LEFT_481_PENALTY=3.790481 PRIMER_RIGHT_481_PENALTY=3.809493 PRIMER_LEFT_481_SEQUENCE=ATTCCCTACCGCTGCTTAGTTG PRIMER_RIGHT_481_SEQUENCE=GGTGTAAGAATTTGCACTTGTCA PRIMER_LEFT_481=536,22 PRIMER_RIGHT_481=611,23 PRIMER_LEFT_481_TM=61.790 PRIMER_RIGHT_481_TM=59.191 PRIMER_LEFT_481_GC_PERCENT=50.000 PRIMER_RIGHT_481_GC_PERCENT=39.130 PRIMER_LEFT_481_SELF_ANY=3.00 PRIMER_RIGHT_481_SELF_ANY=5.00 PRIMER_LEFT_481_SELF_END=1.00 PRIMER_RIGHT_481_SELF_END=3.00 PRIMER_LEFT_481_END_STABILITY=6.7000 PRIMER_RIGHT_481_END_STABILITY=6.7000 PRIMER_PAIR_481_COMPL_ANY=4.00 PRIMER_PAIR_481_COMPL_END=2.00 PRIMER_PAIR_481_PRODUCT_SIZE=76 PRIMER_PAIR_482_PENALTY=7.619819 PRIMER_LEFT_482_PENALTY=3.711172 PRIMER_RIGHT_482_PENALTY=3.908647 PRIMER_LEFT_482_SEQUENCE=CCCTACCGCTGCTTAGTTGGT PRIMER_RIGHT_482_SEQUENCE=CCTGGTGTAAGAATTTGCACTTG PRIMER_LEFT_482=539,21 PRIMER_RIGHT_482=614,23 PRIMER_LEFT_482_TM=62.711 PRIMER_RIGHT_482_TM=60.909 PRIMER_LEFT_482_GC_PERCENT=57.143 PRIMER_RIGHT_482_GC_PERCENT=43.478 PRIMER_LEFT_482_SELF_ANY=3.00 PRIMER_RIGHT_482_SELF_ANY=5.00 PRIMER_LEFT_482_SELF_END=0.00 PRIMER_RIGHT_482_SELF_END=3.00 PRIMER_LEFT_482_END_STABILITY=8.2000 PRIMER_RIGHT_482_END_STABILITY=6.7000 PRIMER_PAIR_482_COMPL_ANY=4.00 PRIMER_PAIR_482_COMPL_END=0.00 PRIMER_PAIR_482_PRODUCT_SIZE=76 PRIMER_PAIR_483_PENALTY=7.651212 PRIMER_LEFT_483_PENALTY=3.527826 PRIMER_RIGHT_483_PENALTY=4.123386 PRIMER_LEFT_483_SEQUENCE=TGAGTTTGTAAGTGATGCCCTTC PRIMER_RIGHT_483_SEQUENCE=GTGCCAGTGAAGATGAGTTTCG PRIMER_LEFT_483=559,23 PRIMER_RIGHT_483=652,22 PRIMER_LEFT_483_TM=60.528 PRIMER_RIGHT_483_TM=62.123 PRIMER_LEFT_483_GC_PERCENT=43.478 PRIMER_RIGHT_483_GC_PERCENT=50.000 PRIMER_LEFT_483_SELF_ANY=4.00 PRIMER_RIGHT_483_SELF_ANY=3.00 PRIMER_LEFT_483_SELF_END=2.00 PRIMER_RIGHT_483_SELF_END=2.00 PRIMER_LEFT_483_END_STABILITY=8.2000 PRIMER_RIGHT_483_END_STABILITY=9.0000 PRIMER_PAIR_483_COMPL_ANY=4.00 PRIMER_PAIR_483_COMPL_END=1.00 PRIMER_PAIR_483_PRODUCT_SIZE=94 PRIMER_PAIR_484_PENALTY=7.656499 PRIMER_LEFT_484_PENALTY=2.936750 PRIMER_RIGHT_484_PENALTY=4.719749 PRIMER_LEFT_484_SEQUENCE=CAGGAGAGGATGGATGTTTGC PRIMER_RIGHT_484_SEQUENCE=TTGGTACTCTTCTCACTGCATGTC PRIMER_LEFT_484=611,21 PRIMER_RIGHT_484=690,24 PRIMER_LEFT_484_TM=61.937 PRIMER_RIGHT_484_TM=60.720 PRIMER_LEFT_484_GC_PERCENT=52.381 PRIMER_RIGHT_484_GC_PERCENT=45.833 PRIMER_LEFT_484_SELF_ANY=2.00 PRIMER_RIGHT_484_SELF_ANY=4.00 PRIMER_LEFT_484_SELF_END=2.00 PRIMER_RIGHT_484_SELF_END=2.00 PRIMER_LEFT_484_END_STABILITY=8.8000 PRIMER_RIGHT_484_END_STABILITY=6.3000 PRIMER_PAIR_484_COMPL_ANY=6.00 PRIMER_PAIR_484_COMPL_END=0.00 PRIMER_PAIR_484_PRODUCT_SIZE=80 PRIMER_PAIR_485_PENALTY=7.656680 PRIMER_LEFT_485_PENALTY=5.256192 PRIMER_RIGHT_485_PENALTY=2.400488 PRIMER_LEFT_485_SEQUENCE=AAAGAGACATGCAGTGAGAAGAGT PRIMER_RIGHT_485_SEQUENCE=AACTCTACCCCTCGGAACTTGT PRIMER_LEFT_485=662,24 PRIMER_RIGHT_485=750,22 PRIMER_LEFT_485_TM=58.744 PRIMER_RIGHT_485_TM=60.400 PRIMER_LEFT_485_GC_PERCENT=41.667 PRIMER_RIGHT_485_GC_PERCENT=50.000 PRIMER_LEFT_485_SELF_ANY=4.00 PRIMER_RIGHT_485_SELF_ANY=3.00 PRIMER_LEFT_485_SELF_END=1.00 PRIMER_RIGHT_485_SELF_END=2.00 PRIMER_LEFT_485_END_STABILITY=6.1000 PRIMER_RIGHT_485_END_STABILITY=6.7000 PRIMER_PAIR_485_COMPL_ANY=5.00 PRIMER_PAIR_485_COMPL_END=2.00 PRIMER_PAIR_485_PRODUCT_SIZE=89 PRIMER_PAIR_486_PENALTY=7.656948 PRIMER_LEFT_486_PENALTY=3.918097 PRIMER_RIGHT_486_PENALTY=3.738851 PRIMER_LEFT_486_SEQUENCE=GCAGATCACCAATGTGGTAGAAG PRIMER_RIGHT_486_SEQUENCE=AAGTGGGGATGGGTCTTG PRIMER_LEFT_486=439,23 PRIMER_RIGHT_486=531,18 PRIMER_LEFT_486_TM=60.918 PRIMER_RIGHT_486_TM=58.261 PRIMER_LEFT_486_GC_PERCENT=47.826 PRIMER_RIGHT_486_GC_PERCENT=55.556 PRIMER_LEFT_486_SELF_ANY=6.00 PRIMER_RIGHT_486_SELF_ANY=3.00 PRIMER_LEFT_486_SELF_END=2.00 PRIMER_RIGHT_486_SELF_END=0.00 PRIMER_LEFT_486_END_STABILITY=6.7000 PRIMER_RIGHT_486_END_STABILITY=7.0000 PRIMER_PAIR_486_COMPL_ANY=5.00 PRIMER_PAIR_486_COMPL_END=2.00 PRIMER_PAIR_486_PRODUCT_SIZE=93 PRIMER_PAIR_487_PENALTY=7.730448 PRIMER_LEFT_487_PENALTY=3.920955 PRIMER_RIGHT_487_PENALTY=3.809493 PRIMER_LEFT_487_SEQUENCE=ATTCCCTACCGCTGCTTAG PRIMER_RIGHT_487_SEQUENCE=TGGTGTAAGAATTTGCACTTGTC PRIMER_LEFT_487=536,19 PRIMER_RIGHT_487=612,23 PRIMER_LEFT_487_TM=57.079 PRIMER_RIGHT_487_TM=59.191 PRIMER_LEFT_487_GC_PERCENT=52.632 PRIMER_RIGHT_487_GC_PERCENT=39.130 PRIMER_LEFT_487_SELF_ANY=3.00 PRIMER_RIGHT_487_SELF_ANY=5.00 PRIMER_LEFT_487_SELF_END=3.00 PRIMER_RIGHT_487_SELF_END=2.00 PRIMER_LEFT_487_END_STABILITY=6.0000 PRIMER_RIGHT_487_END_STABILITY=6.7000 PRIMER_PAIR_487_COMPL_ANY=4.00 PRIMER_PAIR_487_COMPL_END=1.00 PRIMER_PAIR_487_PRODUCT_SIZE=77 PRIMER_PAIR_488_PENALTY=7.771286 PRIMER_LEFT_488_PENALTY=2.908522 PRIMER_RIGHT_488_PENALTY=4.862764 PRIMER_LEFT_488_SEQUENCE=TCATCTTCACTGGCACACC PRIMER_RIGHT_488_SEQUENCE=ACTTGTCAATTCCGCAGG PRIMER_LEFT_488=637,19 PRIMER_RIGHT_488=734,18 PRIMER_LEFT_488_TM=58.091 PRIMER_RIGHT_488_TM=57.137 PRIMER_LEFT_488_GC_PERCENT=52.632 PRIMER_RIGHT_488_GC_PERCENT=50.000 PRIMER_LEFT_488_SELF_ANY=3.00 PRIMER_RIGHT_488_SELF_ANY=5.00 PRIMER_LEFT_488_SELF_END=0.00 PRIMER_RIGHT_488_SELF_END=2.00 PRIMER_LEFT_488_END_STABILITY=7.6000 PRIMER_RIGHT_488_END_STABILITY=9.7000 PRIMER_PAIR_488_COMPL_ANY=3.00 PRIMER_PAIR_488_COMPL_END=2.00 PRIMER_PAIR_488_PRODUCT_SIZE=98 PRIMER_PAIR_489_PENALTY=7.803762 PRIMER_LEFT_489_PENALTY=3.004178 PRIMER_RIGHT_489_PENALTY=4.799584 PRIMER_LEFT_489_SEQUENCE=GGAGAGGATGGATGTTTGC PRIMER_RIGHT_489_SEQUENCE=CAAGTTGGTACTCTTCTCACTGC PRIMER_LEFT_489=613,19 PRIMER_RIGHT_489=694,23 PRIMER_LEFT_489_TM=57.996 PRIMER_RIGHT_489_TM=58.200 PRIMER_LEFT_489_GC_PERCENT=52.632 PRIMER_RIGHT_489_GC_PERCENT=47.826 PRIMER_LEFT_489_SELF_ANY=2.00 PRIMER_RIGHT_489_SELF_ANY=5.00 PRIMER_LEFT_489_SELF_END=2.00 PRIMER_RIGHT_489_SELF_END=2.00 PRIMER_LEFT_489_END_STABILITY=8.8000 PRIMER_RIGHT_489_END_STABILITY=7.9000 PRIMER_PAIR_489_COMPL_ANY=5.00 PRIMER_PAIR_489_COMPL_END=2.00 PRIMER_PAIR_489_PRODUCT_SIZE=82 PRIMER_PAIR_490_PENALTY=7.845623 PRIMER_LEFT_490_PENALTY=4.812325 PRIMER_RIGHT_490_PENALTY=3.033298 PRIMER_LEFT_490_SEQUENCE=AGCAGTGCAAGACCCATC PRIMER_RIGHT_490_SEQUENCE=ACGAGAAGGGCATCACTTACAA PRIMER_LEFT_490=507,18 PRIMER_RIGHT_490=585,22 PRIMER_LEFT_490_TM=57.188 PRIMER_RIGHT_490_TM=61.033 PRIMER_LEFT_490_GC_PERCENT=55.556 PRIMER_RIGHT_490_GC_PERCENT=45.455 PRIMER_LEFT_490_SELF_ANY=5.00 PRIMER_RIGHT_490_SELF_ANY=4.00 PRIMER_LEFT_490_SELF_END=0.00 PRIMER_RIGHT_490_SELF_END=2.00 PRIMER_LEFT_490_END_STABILITY=8.1000 PRIMER_RIGHT_490_END_STABILITY=6.0000 PRIMER_PAIR_490_COMPL_ANY=5.00 PRIMER_PAIR_490_COMPL_END=2.00 PRIMER_PAIR_490_PRODUCT_SIZE=79 PRIMER_PAIR_491_PENALTY=7.857702 PRIMER_LEFT_491_PENALTY=3.258282 PRIMER_RIGHT_491_PENALTY=4.599420 PRIMER_LEFT_491_SEQUENCE=GTGCAAATTCTTACACCAGGA PRIMER_RIGHT_491_SEQUENCE=CTCTTCTCACTGCATGTCTCTTTG PRIMER_LEFT_491=595,21 PRIMER_RIGHT_491=684,24 PRIMER_LEFT_491_TM=57.742 PRIMER_RIGHT_491_TM=60.599 PRIMER_LEFT_491_GC_PERCENT=42.857 PRIMER_RIGHT_491_GC_PERCENT=45.833 PRIMER_LEFT_491_SELF_ANY=5.00 PRIMER_RIGHT_491_SELF_ANY=4.00 PRIMER_LEFT_491_SELF_END=1.00 PRIMER_RIGHT_491_SELF_END=0.00 PRIMER_LEFT_491_END_STABILITY=8.2000 PRIMER_RIGHT_491_END_STABILITY=7.3000 PRIMER_PAIR_491_COMPL_ANY=4.00 PRIMER_PAIR_491_COMPL_END=2.00 PRIMER_PAIR_491_PRODUCT_SIZE=90 PRIMER_PAIR_492_PENALTY=7.867014 PRIMER_LEFT_492_PENALTY=2.207202 PRIMER_RIGHT_492_PENALTY=5.659812 PRIMER_LEFT_492_SEQUENCE=GAATGGGAAGTGGGATTCAGA PRIMER_RIGHT_492_SEQUENCE=CTGCAGTTCAGGGTAGACTTCTTG PRIMER_LEFT_492=343,21 PRIMER_RIGHT_492=442,24 PRIMER_LEFT_492_TM=61.207 PRIMER_RIGHT_492_TM=61.660 PRIMER_LEFT_492_GC_PERCENT=47.619 PRIMER_RIGHT_492_GC_PERCENT=50.000 PRIMER_LEFT_492_SELF_ANY=4.00 PRIMER_RIGHT_492_SELF_ANY=6.00 PRIMER_LEFT_492_SELF_END=3.00 PRIMER_RIGHT_492_SELF_END=1.00 PRIMER_LEFT_492_END_STABILITY=6.7000 PRIMER_RIGHT_492_END_STABILITY=7.0000 PRIMER_PAIR_492_COMPL_ANY=5.00 PRIMER_PAIR_492_COMPL_END=2.00 PRIMER_PAIR_492_PRODUCT_SIZE=100 PRIMER_PAIR_493_PENALTY=7.998550 PRIMER_LEFT_493_PENALTY=3.123340 PRIMER_RIGHT_493_PENALTY=4.875209 PRIMER_LEFT_493_SEQUENCE=ATGCACATGAATGTCCAGAAT PRIMER_RIGHT_493_SEQUENCE=TTCCTTGGTATCAATGCAGGTTT PRIMER_LEFT_493=326,21 PRIMER_RIGHT_493=400,23 PRIMER_LEFT_493_TM=57.877 PRIMER_RIGHT_493_TM=61.875 PRIMER_LEFT_493_GC_PERCENT=38.095 PRIMER_RIGHT_493_GC_PERCENT=39.130 PRIMER_LEFT_493_SELF_ANY=8.00 PRIMER_RIGHT_493_SELF_ANY=6.00 PRIMER_LEFT_493_SELF_END=2.00 PRIMER_RIGHT_493_SELF_END=1.00 PRIMER_LEFT_493_END_STABILITY=6.6000 PRIMER_RIGHT_493_END_STABILITY=8.2000 PRIMER_PAIR_493_COMPL_ANY=4.00 PRIMER_PAIR_493_COMPL_END=1.00 PRIMER_PAIR_493_PRODUCT_SIZE=75 PRIMER_PAIR_494_PENALTY=8.009088 PRIMER_LEFT_494_PENALTY=4.320176 PRIMER_RIGHT_494_PENALTY=3.688912 PRIMER_LEFT_494_SEQUENCE=TCCCCACTTTGTGATTCCCTAC PRIMER_RIGHT_494_SEQUENCE=GAATTTGCACTTGTCAGGAAC PRIMER_LEFT_494=523,22 PRIMER_RIGHT_494=604,21 PRIMER_LEFT_494_TM=62.320 PRIMER_RIGHT_494_TM=57.311 PRIMER_LEFT_494_GC_PERCENT=50.000 PRIMER_RIGHT_494_GC_PERCENT=42.857 PRIMER_LEFT_494_SELF_ANY=3.00 PRIMER_RIGHT_494_SELF_ANY=4.00 PRIMER_LEFT_494_SELF_END=1.00 PRIMER_RIGHT_494_SELF_END=1.00 PRIMER_LEFT_494_END_STABILITY=6.9000 PRIMER_RIGHT_494_END_STABILITY=7.9000 PRIMER_PAIR_494_COMPL_ANY=4.00 PRIMER_PAIR_494_COMPL_END=3.00 PRIMER_PAIR_494_PRODUCT_SIZE=82 PRIMER_PAIR_495_PENALTY=8.018685 PRIMER_LEFT_495_PENALTY=4.985387 PRIMER_RIGHT_495_PENALTY=3.033298 PRIMER_LEFT_495_SEQUENCE=TGCAAGACCCATCCCCAC PRIMER_RIGHT_495_SEQUENCE=AACGAGAAGGGCATCACTTACA PRIMER_LEFT_495=512,18 PRIMER_RIGHT_495=586,22 PRIMER_LEFT_495_TM=62.985 PRIMER_RIGHT_495_TM=61.033 PRIMER_LEFT_495_GC_PERCENT=61.111 PRIMER_RIGHT_495_GC_PERCENT=45.455 PRIMER_LEFT_495_SELF_ANY=4.00 PRIMER_RIGHT_495_SELF_ANY=4.00 PRIMER_LEFT_495_SELF_END=0.00 PRIMER_RIGHT_495_SELF_END=0.00 PRIMER_LEFT_495_END_STABILITY=9.4000 PRIMER_RIGHT_495_END_STABILITY=6.0000 PRIMER_PAIR_495_COMPL_ANY=4.00 PRIMER_PAIR_495_COMPL_END=0.00 PRIMER_PAIR_495_PRODUCT_SIZE=75 PRIMER_PAIR_496_PENALTY=8.033437 PRIMER_LEFT_496_PENALTY=6.544143 PRIMER_RIGHT_496_PENALTY=1.489294 PRIMER_LEFT_496_SEQUENCE=AAGAGACATGCAGTGAGAAGAGTA PRIMER_RIGHT_496_SEQUENCE=GGGCAACACACAAACTCTACC PRIMER_LEFT_496=663,24 PRIMER_RIGHT_496=762,21 PRIMER_LEFT_496_TM=57.456 PRIMER_RIGHT_496_TM=59.511 PRIMER_LEFT_496_GC_PERCENT=41.667 PRIMER_RIGHT_496_GC_PERCENT=52.381 PRIMER_LEFT_496_SELF_ANY=4.00 PRIMER_RIGHT_496_SELF_ANY=2.00 PRIMER_LEFT_496_SELF_END=2.00 PRIMER_RIGHT_496_SELF_END=0.00 PRIMER_LEFT_496_END_STABILITY=5.4000 PRIMER_RIGHT_496_END_STABILITY=6.9000 PRIMER_PAIR_496_COMPL_ANY=5.00 PRIMER_PAIR_496_COMPL_END=3.00 PRIMER_PAIR_496_PRODUCT_SIZE=100 PRIMER_PAIR_497_PENALTY=8.052866 PRIMER_LEFT_497_PENALTY=5.652377 PRIMER_RIGHT_497_PENALTY=2.400488 PRIMER_LEFT_497_SEQUENCE=AAGAGACATGCAGTGAGAAGAGT PRIMER_RIGHT_497_SEQUENCE=ACAAACTCTACCCCTCGGAACT PRIMER_LEFT_497=663,23 PRIMER_RIGHT_497=753,22 PRIMER_LEFT_497_TM=57.348 PRIMER_RIGHT_497_TM=60.400 PRIMER_LEFT_497_GC_PERCENT=43.478 PRIMER_RIGHT_497_GC_PERCENT=50.000 PRIMER_LEFT_497_SELF_ANY=4.00 PRIMER_RIGHT_497_SELF_ANY=3.00 PRIMER_LEFT_497_SELF_END=1.00 PRIMER_RIGHT_497_SELF_END=1.00 PRIMER_LEFT_497_END_STABILITY=6.1000 PRIMER_RIGHT_497_END_STABILITY=6.4000 PRIMER_PAIR_497_COMPL_ANY=5.00 PRIMER_PAIR_497_COMPL_END=3.00 PRIMER_PAIR_497_PRODUCT_SIZE=91 PRIMER_PAIR_498_PENALTY=8.056021 PRIMER_LEFT_498_PENALTY=3.256437 PRIMER_RIGHT_498_PENALTY=4.799584 PRIMER_LEFT_498_SEQUENCE=TTACACCAGGAGAGGATGGATG PRIMER_RIGHT_498_SEQUENCE=GCAAGTTGGTACTCTTCTCACTG PRIMER_LEFT_498=605,22 PRIMER_RIGHT_498=695,23 PRIMER_LEFT_498_TM=61.256 PRIMER_RIGHT_498_TM=58.200 PRIMER_LEFT_498_GC_PERCENT=50.000 PRIMER_RIGHT_498_GC_PERCENT=47.826 PRIMER_LEFT_498_SELF_ANY=3.00 PRIMER_RIGHT_498_SELF_ANY=5.00 PRIMER_LEFT_498_SELF_END=0.00 PRIMER_RIGHT_498_SELF_END=3.00 PRIMER_LEFT_498_END_STABILITY=8.1000 PRIMER_RIGHT_498_END_STABILITY=6.7000 PRIMER_PAIR_498_COMPL_ANY=6.00 PRIMER_PAIR_498_COMPL_END=3.00 PRIMER_PAIR_498_PRODUCT_SIZE=91 PRIMER_PAIR_499_PENALTY=8.090730 PRIMER_LEFT_499_PENALTY=5.604473 PRIMER_RIGHT_499_PENALTY=2.486257 PRIMER_LEFT_499_SEQUENCE=AACATGCACATGAATGTCCAGAA PRIMER_RIGHT_499_SEQUENCE=CTTGGTATCAATGCAGGTTTTG PRIMER_LEFT_499=323,23 PRIMER_RIGHT_499=397,22 PRIMER_LEFT_499_TM=62.604 PRIMER_RIGHT_499_TM=59.514 PRIMER_LEFT_499_GC_PERCENT=39.130 PRIMER_RIGHT_499_GC_PERCENT=40.909 PRIMER_LEFT_499_SELF_ANY=8.00 PRIMER_RIGHT_499_SELF_ANY=6.00 PRIMER_LEFT_499_SELF_END=2.00 PRIMER_RIGHT_499_SELF_END=2.00 PRIMER_LEFT_499_END_STABILITY=7.0000 PRIMER_RIGHT_499_END_STABILITY=7.6000 PRIMER_PAIR_499_COMPL_ANY=6.00 PRIMER_PAIR_499_COMPL_END=3.00 PRIMER_PAIR_499_PRODUCT_SIZE=75 PRIMER_PAIR_500_PENALTY=8.120276 PRIMER_LEFT_500_PENALTY=3.296517 PRIMER_RIGHT_500_PENALTY=4.823759 PRIMER_LEFT_500_SEQUENCE=AGTGCAAATTCTTACACCAGGAG PRIMER_RIGHT_500_SEQUENCE=TCTCACTGCATGTCTCTTTGGC PRIMER_LEFT_500=594,23 PRIMER_RIGHT_500=680,22 PRIMER_LEFT_500_TM=59.703 PRIMER_RIGHT_500_TM=62.824 PRIMER_LEFT_500_GC_PERCENT=43.478 PRIMER_RIGHT_500_GC_PERCENT=50.000 PRIMER_LEFT_500_SELF_ANY=5.00 PRIMER_RIGHT_500_SELF_ANY=4.00 PRIMER_LEFT_500_SELF_END=3.00 PRIMER_RIGHT_500_SELF_END=2.00 PRIMER_LEFT_500_END_STABILITY=7.9000 PRIMER_RIGHT_500_END_STABILITY=10.0000 PRIMER_PAIR_500_COMPL_ANY=4.00 PRIMER_PAIR_500_COMPL_END=2.00 PRIMER_PAIR_500_PRODUCT_SIZE=87 PRIMER_PAIR_501_PENALTY=8.143311 PRIMER_LEFT_501_PENALTY=4.366422 PRIMER_RIGHT_501_PENALTY=3.776889 PRIMER_LEFT_501_SEQUENCE=ACCAATGTGGTAGAAGCCAACC PRIMER_RIGHT_501_SEQUENCE=GGTCTTGCACTGCTTGCG PRIMER_LEFT_501=446,22 PRIMER_RIGHT_501=520,18 PRIMER_LEFT_501_TM=62.366 PRIMER_RIGHT_501_TM=61.777 PRIMER_LEFT_501_GC_PERCENT=50.000 PRIMER_RIGHT_501_GC_PERCENT=61.111 PRIMER_LEFT_501_SELF_ANY=6.00 PRIMER_RIGHT_501_SELF_ANY=5.00 PRIMER_LEFT_501_SELF_END=2.00 PRIMER_RIGHT_501_SELF_END=2.00 PRIMER_LEFT_501_END_STABILITY=8.2000 PRIMER_RIGHT_501_END_STABILITY=10.5000 PRIMER_PAIR_501_COMPL_ANY=4.00 PRIMER_PAIR_501_COMPL_END=2.00 PRIMER_PAIR_501_PRODUCT_SIZE=75 PRIMER_PAIR_502_PENALTY=8.145270 PRIMER_LEFT_502_PENALTY=3.655999 PRIMER_RIGHT_502_PENALTY=4.489271 PRIMER_LEFT_502_SEQUENCE=TGAGTTTGTAAGTGATGCCCT PRIMER_RIGHT_502_SEQUENCE=TTCGCAAACATCCATCCT PRIMER_LEFT_502=559,21 PRIMER_RIGHT_502=634,18 PRIMER_LEFT_502_TM=57.344 PRIMER_RIGHT_502_TM=57.511 PRIMER_LEFT_502_GC_PERCENT=42.857 PRIMER_RIGHT_502_GC_PERCENT=44.444 PRIMER_LEFT_502_SELF_ANY=2.00 PRIMER_RIGHT_502_SELF_ANY=4.00 PRIMER_LEFT_502_SELF_END=2.00 PRIMER_RIGHT_502_SELF_END=0.00 PRIMER_LEFT_502_END_STABILITY=10.9000 PRIMER_RIGHT_502_END_STABILITY=7.8000 PRIMER_PAIR_502_COMPL_ANY=5.00 PRIMER_PAIR_502_COMPL_END=1.00 PRIMER_PAIR_502_PRODUCT_SIZE=76 PRIMER_PAIR_503_PENALTY=8.156303 PRIMER_LEFT_503_PENALTY=4.399603 PRIMER_RIGHT_503_PENALTY=3.756700 PRIMER_LEFT_503_SEQUENCE=TGCAGTATTGCCAAGAAGTCTAC PRIMER_RIGHT_503_SEQUENCE=TCTGGATGGTCACTGGTTGGT PRIMER_LEFT_503=408,23 PRIMER_RIGHT_503=485,21 PRIMER_LEFT_503_TM=58.600 PRIMER_RIGHT_503_TM=62.757 PRIMER_LEFT_503_GC_PERCENT=43.478 PRIMER_RIGHT_503_GC_PERCENT=52.381 PRIMER_LEFT_503_SELF_ANY=6.00 PRIMER_RIGHT_503_SELF_ANY=3.00 PRIMER_LEFT_503_SELF_END=3.00 PRIMER_RIGHT_503_SELF_END=0.00 PRIMER_LEFT_503_END_STABILITY=5.4000 PRIMER_RIGHT_503_END_STABILITY=8.2000 PRIMER_PAIR_503_COMPL_ANY=5.00 PRIMER_PAIR_503_COMPL_END=3.00 PRIMER_PAIR_503_PRODUCT_SIZE=78 PRIMER_PAIR_504_PENALTY=8.156360 PRIMER_LEFT_504_PENALTY=3.161374 PRIMER_RIGHT_504_PENALTY=4.994987 PRIMER_LEFT_504_SEQUENCE=GATGTTTGCGAAACTCATCTTC PRIMER_RIGHT_504_SEQUENCE=CGTAGTCATGCAAGTTGGTACT PRIMER_LEFT_504=623,22 PRIMER_RIGHT_504=704,22 PRIMER_LEFT_504_TM=58.839 PRIMER_RIGHT_504_TM=57.005 PRIMER_LEFT_504_GC_PERCENT=40.909 PRIMER_RIGHT_504_GC_PERCENT=45.455 PRIMER_LEFT_504_SELF_ANY=6.00 PRIMER_RIGHT_504_SELF_ANY=5.00 PRIMER_LEFT_504_SELF_END=3.00 PRIMER_RIGHT_504_SELF_END=3.00 PRIMER_LEFT_504_END_STABILITY=6.7000 PRIMER_RIGHT_504_END_STABILITY=5.1000 PRIMER_PAIR_504_COMPL_ANY=4.00 PRIMER_PAIR_504_COMPL_END=1.00 PRIMER_PAIR_504_PRODUCT_SIZE=82 PRIMER_PAIR_505_PENALTY=8.165522 PRIMER_LEFT_505_PENALTY=4.705093 PRIMER_RIGHT_505_PENALTY=3.460430 PRIMER_LEFT_505_SEQUENCE=GGAGGTACCCACTGATGGTAATG PRIMER_RIGHT_505_SEQUENCE=ACATTCATGTGCATGTTCAGTCT PRIMER_LEFT_505=253,23 PRIMER_RIGHT_505=339,23 PRIMER_LEFT_505_TM=61.705 PRIMER_RIGHT_505_TM=59.540 PRIMER_LEFT_505_GC_PERCENT=52.174 PRIMER_RIGHT_505_GC_PERCENT=39.130 PRIMER_LEFT_505_SELF_ANY=6.00 PRIMER_RIGHT_505_SELF_ANY=8.00 PRIMER_LEFT_505_SELF_END=0.00 PRIMER_RIGHT_505_SELF_END=3.00 PRIMER_LEFT_505_END_STABILITY=6.2000 PRIMER_RIGHT_505_END_STABILITY=6.1000 PRIMER_PAIR_505_COMPL_ANY=5.00 PRIMER_PAIR_505_COMPL_END=1.00 PRIMER_PAIR_505_PRODUCT_SIZE=87 PRIMER_PAIR_506_PENALTY=8.171832 PRIMER_LEFT_506_PENALTY=4.111908 PRIMER_RIGHT_506_PENALTY=4.059923 PRIMER_LEFT_506_SEQUENCE=GCTGCTTAGTTGGTGAGTTTGTAA PRIMER_RIGHT_506_SEQUENCE=AACATCCATCCTCTCCTGGTGT PRIMER_LEFT_506=546,24 PRIMER_RIGHT_506=628,22 PRIMER_LEFT_506_TM=59.888 PRIMER_RIGHT_506_TM=62.060 PRIMER_LEFT_506_GC_PERCENT=41.667 PRIMER_RIGHT_506_GC_PERCENT=50.000 PRIMER_LEFT_506_SELF_ANY=3.00 PRIMER_RIGHT_506_SELF_ANY=4.00 PRIMER_LEFT_506_SELF_END=2.00 PRIMER_RIGHT_506_SELF_END=1.00 PRIMER_LEFT_506_END_STABILITY=6.0000 PRIMER_RIGHT_506_END_STABILITY=7.6000 PRIMER_PAIR_506_COMPL_ANY=4.00 PRIMER_PAIR_506_COMPL_END=1.00 PRIMER_PAIR_506_PRODUCT_SIZE=83 PRIMER_PAIR_507_PENALTY=8.185582 PRIMER_LEFT_507_PENALTY=4.705093 PRIMER_RIGHT_507_PENALTY=3.480490 PRIMER_LEFT_507_SEQUENCE=TGGAGGTACCCACTGATGGTAAT PRIMER_RIGHT_507_SEQUENCE=CATTCATGTGCATGTTCAGTC PRIMER_LEFT_507=252,23 PRIMER_RIGHT_507=338,21 PRIMER_LEFT_507_TM=61.705 PRIMER_RIGHT_507_TM=57.520 PRIMER_LEFT_507_GC_PERCENT=47.826 PRIMER_RIGHT_507_GC_PERCENT=42.857 PRIMER_LEFT_507_SELF_ANY=6.00 PRIMER_RIGHT_507_SELF_ANY=6.00 PRIMER_LEFT_507_SELF_END=2.00 PRIMER_RIGHT_507_SELF_END=1.00 PRIMER_LEFT_507_END_STABILITY=5.6000 PRIMER_RIGHT_507_END_STABILITY=6.4000 PRIMER_PAIR_507_COMPL_ANY=5.00 PRIMER_PAIR_507_COMPL_END=1.00 PRIMER_PAIR_507_PRODUCT_SIZE=87 PRIMER_PAIR_508_PENALTY=8.199312 PRIMER_LEFT_508_PENALTY=4.326727 PRIMER_RIGHT_508_PENALTY=3.872586 PRIMER_LEFT_508_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_508_SEQUENCE=CCAGTTCTGGATGGTCACTGG PRIMER_LEFT_508=414,23 PRIMER_RIGHT_508=490,21 PRIMER_LEFT_508_TM=61.327 PRIMER_RIGHT_508_TM=62.873 PRIMER_LEFT_508_GC_PERCENT=43.478 PRIMER_RIGHT_508_GC_PERCENT=57.143 PRIMER_LEFT_508_SELF_ANY=5.00 PRIMER_RIGHT_508_SELF_ANY=6.00 PRIMER_LEFT_508_SELF_END=0.00 PRIMER_RIGHT_508_SELF_END=1.00 PRIMER_LEFT_508_END_STABILITY=7.0000 PRIMER_RIGHT_508_END_STABILITY=7.9000 PRIMER_PAIR_508_COMPL_ANY=4.00 PRIMER_PAIR_508_COMPL_END=3.00 PRIMER_PAIR_508_PRODUCT_SIZE=77 PRIMER_PAIR_509_PENALTY=8.200452 PRIMER_LEFT_509_PENALTY=4.984244 PRIMER_RIGHT_509_PENALTY=3.216208 PRIMER_LEFT_509_SEQUENCE=GCTGGAGGTACCCACTGA PRIMER_RIGHT_509_SEQUENCE=GTTCAGTCTGCCACAGAACAT PRIMER_LEFT_509=250,18 PRIMER_RIGHT_509=325,21 PRIMER_LEFT_509_TM=57.016 PRIMER_RIGHT_509_TM=57.784 PRIMER_LEFT_509_GC_PERCENT=61.111 PRIMER_RIGHT_509_GC_PERCENT=47.619 PRIMER_LEFT_509_SELF_ANY=6.00 PRIMER_RIGHT_509_SELF_ANY=6.00 PRIMER_LEFT_509_SELF_END=2.00 PRIMER_RIGHT_509_SELF_END=2.00 PRIMER_LEFT_509_END_STABILITY=6.4000 PRIMER_RIGHT_509_END_STABILITY=6.6000 PRIMER_PAIR_509_COMPL_ANY=5.00 PRIMER_PAIR_509_COMPL_END=1.00 PRIMER_PAIR_509_PRODUCT_SIZE=76 PRIMER_PAIR_510_PENALTY=8.227696 PRIMER_LEFT_510_PENALTY=2.227266 PRIMER_RIGHT_510_PENALTY=6.000430 PRIMER_LEFT_510_SEQUENCE=GTCCAGAATGGGAAGTGGGA PRIMER_RIGHT_510_SEQUENCE=GTTCAGGGTAGACTTCTTGGCAAT PRIMER_LEFT_510=338,20 PRIMER_RIGHT_510=437,24 PRIMER_LEFT_510_TM=62.227 PRIMER_RIGHT_510_TM=62.000 PRIMER_LEFT_510_GC_PERCENT=55.000 PRIMER_RIGHT_510_GC_PERCENT=45.833 PRIMER_LEFT_510_SELF_ANY=3.00 PRIMER_RIGHT_510_SELF_ANY=5.00 PRIMER_LEFT_510_SELF_END=0.00 PRIMER_RIGHT_510_SELF_END=3.00 PRIMER_LEFT_510_END_STABILITY=9.7000 PRIMER_RIGHT_510_END_STABILITY=8.4000 PRIMER_PAIR_510_COMPL_ANY=5.00 PRIMER_PAIR_510_COMPL_END=1.00 PRIMER_PAIR_510_PRODUCT_SIZE=100 PRIMER_PAIR_511_PENALTY=8.239160 PRIMER_LEFT_511_PENALTY=4.089746 PRIMER_RIGHT_511_PENALTY=4.149413 PRIMER_LEFT_511_SEQUENCE=AGGGACCAAAACCTGCAT PRIMER_RIGHT_511_SEQUENCE=TTGGTTGGCTTCTACCACATTG PRIMER_LEFT_511=370,18 PRIMER_RIGHT_511=469,22 PRIMER_LEFT_511_TM=57.910 PRIMER_RIGHT_511_TM=62.149 PRIMER_LEFT_511_GC_PERCENT=50.000 PRIMER_RIGHT_511_GC_PERCENT=45.455 PRIMER_LEFT_511_SELF_ANY=4.00 PRIMER_RIGHT_511_SELF_ANY=4.00 PRIMER_LEFT_511_SELF_END=2.00 PRIMER_RIGHT_511_SELF_END=3.00 PRIMER_LEFT_511_END_STABILITY=8.4000 PRIMER_RIGHT_511_END_STABILITY=7.2000 PRIMER_PAIR_511_COMPL_ANY=5.00 PRIMER_PAIR_511_COMPL_END=1.00 PRIMER_PAIR_511_PRODUCT_SIZE=100 PRIMER_PAIR_512_PENALTY=8.261706 PRIMER_LEFT_512_PENALTY=4.166335 PRIMER_RIGHT_512_PENALTY=4.095371 PRIMER_LEFT_512_SEQUENCE=GATTCCCTACCGCTGCTT PRIMER_RIGHT_512_SEQUENCE=GTGTAAGAATTTGCACTTGTCAGG PRIMER_LEFT_512=535,18 PRIMER_RIGHT_512=610,24 PRIMER_LEFT_512_TM=57.834 PRIMER_RIGHT_512_TM=60.095 PRIMER_LEFT_512_GC_PERCENT=55.556 PRIMER_RIGHT_512_GC_PERCENT=41.667 PRIMER_LEFT_512_SELF_ANY=3.00 PRIMER_RIGHT_512_SELF_ANY=5.00 PRIMER_LEFT_512_SELF_END=0.00 PRIMER_RIGHT_512_SELF_END=3.00 PRIMER_LEFT_512_END_STABILITY=8.5000 PRIMER_RIGHT_512_END_STABILITY=8.2000 PRIMER_PAIR_512_COMPL_ANY=4.00 PRIMER_PAIR_512_COMPL_END=1.00 PRIMER_PAIR_512_PRODUCT_SIZE=76 PRIMER_PAIR_513_PENALTY=8.271289 PRIMER_LEFT_513_PENALTY=4.482644 PRIMER_RIGHT_513_PENALTY=3.788644 PRIMER_LEFT_513_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTAC PRIMER_RIGHT_513_SEQUENCE=GATGGTCACTGGTTGGTTG PRIMER_LEFT_513=407,24 PRIMER_RIGHT_513=481,19 PRIMER_LEFT_513_TM=59.517 PRIMER_RIGHT_513_TM=57.211 PRIMER_LEFT_513_GC_PERCENT=45.833 PRIMER_RIGHT_513_GC_PERCENT=52.632 PRIMER_LEFT_513_SELF_ANY=6.00 PRIMER_RIGHT_513_SELF_ANY=3.00 PRIMER_LEFT_513_SELF_END=3.00 PRIMER_RIGHT_513_SELF_END=0.00 PRIMER_LEFT_513_END_STABILITY=5.4000 PRIMER_RIGHT_513_END_STABILITY=8.2000 PRIMER_PAIR_513_COMPL_ANY=5.00 PRIMER_PAIR_513_COMPL_END=2.00 PRIMER_PAIR_513_PRODUCT_SIZE=75 PRIMER_PAIR_514_PENALTY=8.297501 PRIMER_LEFT_514_PENALTY=4.198427 PRIMER_RIGHT_514_PENALTY=4.099075 PRIMER_LEFT_514_SEQUENCE=CGCTGCTTAGTTGGTGAGTTTG PRIMER_RIGHT_514_SEQUENCE=ATCCTCTCCTGGTGTAAGAATTTG PRIMER_LEFT_514=545,22 PRIMER_RIGHT_514=621,24 PRIMER_LEFT_514_TM=62.198 PRIMER_RIGHT_514_TM=59.901 PRIMER_LEFT_514_GC_PERCENT=50.000 PRIMER_RIGHT_514_GC_PERCENT=41.667 PRIMER_LEFT_514_SELF_ANY=3.00 PRIMER_RIGHT_514_SELF_ANY=4.00 PRIMER_LEFT_514_SELF_END=0.00 PRIMER_RIGHT_514_SELF_END=1.00 PRIMER_LEFT_514_END_STABILITY=7.0000 PRIMER_RIGHT_514_END_STABILITY=7.2000 PRIMER_PAIR_514_COMPL_ANY=4.00 PRIMER_PAIR_514_COMPL_END=0.00 PRIMER_PAIR_514_PRODUCT_SIZE=77 PRIMER_PAIR_515_PENALTY=8.319889 PRIMER_LEFT_515_PENALTY=5.713033 PRIMER_RIGHT_515_PENALTY=2.606855 PRIMER_LEFT_515_SEQUENCE=GTACCCACTGATGGTAATGCTGG PRIMER_RIGHT_515_SEQUENCE=CCCACTTCCCATTCTGGA PRIMER_LEFT_515=257,23 PRIMER_RIGHT_515=356,18 PRIMER_LEFT_515_TM=62.713 PRIMER_RIGHT_515_TM=59.393 PRIMER_LEFT_515_GC_PERCENT=52.174 PRIMER_RIGHT_515_GC_PERCENT=55.556 PRIMER_LEFT_515_SELF_ANY=5.00 PRIMER_RIGHT_515_SELF_ANY=3.00 PRIMER_LEFT_515_SELF_END=0.00 PRIMER_RIGHT_515_SELF_END=2.00 PRIMER_LEFT_515_END_STABILITY=9.7000 PRIMER_RIGHT_515_END_STABILITY=8.2000 PRIMER_PAIR_515_COMPL_ANY=5.00 PRIMER_PAIR_515_COMPL_END=3.00 PRIMER_PAIR_515_PRODUCT_SIZE=100 PRIMER_PAIR_516_PENALTY=8.321049 PRIMER_LEFT_516_PENALTY=4.840560 PRIMER_RIGHT_516_PENALTY=3.480490 PRIMER_LEFT_516_SEQUENCE=GAGGTACCCACTGATGGTAATGC PRIMER_RIGHT_516_SEQUENCE=GACATTCATGTGCATGTTCAG PRIMER_LEFT_516=254,23 PRIMER_RIGHT_516=340,21 PRIMER_LEFT_516_TM=61.841 PRIMER_RIGHT_516_TM=57.520 PRIMER_LEFT_516_GC_PERCENT=52.174 PRIMER_RIGHT_516_GC_PERCENT=42.857 PRIMER_LEFT_516_SELF_ANY=6.00 PRIMER_RIGHT_516_SELF_ANY=8.00 PRIMER_LEFT_516_SELF_END=2.00 PRIMER_RIGHT_516_SELF_END=2.00 PRIMER_LEFT_516_END_STABILITY=8.4000 PRIMER_RIGHT_516_END_STABILITY=7.0000 PRIMER_PAIR_516_COMPL_ANY=4.00 PRIMER_PAIR_516_COMPL_END=2.00 PRIMER_PAIR_516_PRODUCT_SIZE=87 PRIMER_PAIR_517_PENALTY=8.327517 PRIMER_LEFT_517_PENALTY=3.747215 PRIMER_RIGHT_517_PENALTY=4.580302 PRIMER_LEFT_517_SEQUENCE=GTGAGTTTGTAAGTGATGCCCT PRIMER_RIGHT_517_SEQUENCE=GTTTCGCAAACATCCATCCTCT PRIMER_LEFT_517=558,22 PRIMER_RIGHT_517=636,22 PRIMER_LEFT_517_TM=58.253 PRIMER_RIGHT_517_TM=62.580 PRIMER_LEFT_517_GC_PERCENT=45.455 PRIMER_RIGHT_517_GC_PERCENT=45.455 PRIMER_LEFT_517_SELF_ANY=2.00 PRIMER_RIGHT_517_SELF_ANY=6.00 PRIMER_LEFT_517_SELF_END=2.00 PRIMER_RIGHT_517_SELF_END=0.00 PRIMER_LEFT_517_END_STABILITY=10.9000 PRIMER_RIGHT_517_END_STABILITY=7.9000 PRIMER_PAIR_517_COMPL_ANY=5.00 PRIMER_PAIR_517_COMPL_END=0.00 PRIMER_PAIR_517_PRODUCT_SIZE=79 PRIMER_PAIR_518_PENALTY=8.341972 PRIMER_LEFT_518_PENALTY=5.168862 PRIMER_RIGHT_518_PENALTY=3.173109 PRIMER_LEFT_518_SEQUENCE=GTAGAAGCCAACCAACCAGTGAC PRIMER_RIGHT_518_SEQUENCE=GCAGCGGTAGGGAATCACA PRIMER_LEFT_518=455,23 PRIMER_RIGHT_518=550,19 PRIMER_LEFT_518_TM=62.169 PRIMER_RIGHT_518_TM=62.173 PRIMER_LEFT_518_GC_PERCENT=52.174 PRIMER_RIGHT_518_GC_PERCENT=57.895 PRIMER_LEFT_518_SELF_ANY=3.00 PRIMER_RIGHT_518_SELF_ANY=3.00 PRIMER_LEFT_518_SELF_END=3.00 PRIMER_RIGHT_518_SELF_END=1.00 PRIMER_LEFT_518_END_STABILITY=6.1000 PRIMER_RIGHT_518_END_STABILITY=6.7000 PRIMER_PAIR_518_COMPL_ANY=4.00 PRIMER_PAIR_518_COMPL_END=2.00 PRIMER_PAIR_518_PRODUCT_SIZE=96 PRIMER_PAIR_519_PENALTY=8.349744 PRIMER_LEFT_519_PENALTY=4.610893 PRIMER_RIGHT_519_PENALTY=3.738851 PRIMER_LEFT_519_SEQUENCE=AGATCACCAATGTGGTAGAAGCC PRIMER_RIGHT_519_SEQUENCE=CAAAGTGGGGATGGGTCT PRIMER_LEFT_519=441,23 PRIMER_RIGHT_519=533,18 PRIMER_LEFT_519_TM=61.611 PRIMER_RIGHT_519_TM=58.261 PRIMER_LEFT_519_GC_PERCENT=47.826 PRIMER_RIGHT_519_GC_PERCENT=55.556 PRIMER_LEFT_519_SELF_ANY=6.00 PRIMER_RIGHT_519_SELF_ANY=2.00 PRIMER_LEFT_519_SELF_END=1.00 PRIMER_RIGHT_519_SELF_END=0.00 PRIMER_LEFT_519_END_STABILITY=9.7000 PRIMER_RIGHT_519_END_STABILITY=7.6000 PRIMER_PAIR_519_COMPL_ANY=6.00 PRIMER_PAIR_519_COMPL_END=2.00 PRIMER_PAIR_519_PRODUCT_SIZE=93 PRIMER_PAIR_520_PENALTY=8.361511 PRIMER_LEFT_520_PENALTY=4.293610 PRIMER_RIGHT_520_PENALTY=4.067900 PRIMER_LEFT_520_SEQUENCE=GTGATTCCCTACCGCTGCTTAG PRIMER_RIGHT_520_SEQUENCE=TAAGAATTTGCACTTGTCAGGAA PRIMER_LEFT_520=533,22 PRIMER_RIGHT_520=607,23 PRIMER_LEFT_520_TM=62.294 PRIMER_RIGHT_520_TM=58.932 PRIMER_LEFT_520_GC_PERCENT=54.545 PRIMER_RIGHT_520_GC_PERCENT=34.783 PRIMER_LEFT_520_SELF_ANY=3.00 PRIMER_RIGHT_520_SELF_ANY=4.00 PRIMER_LEFT_520_SELF_END=3.00 PRIMER_RIGHT_520_SELF_END=0.00 PRIMER_LEFT_520_END_STABILITY=6.0000 PRIMER_RIGHT_520_END_STABILITY=8.2000 PRIMER_PAIR_520_COMPL_ANY=4.00 PRIMER_PAIR_520_COMPL_END=2.00 PRIMER_PAIR_520_PRODUCT_SIZE=75 PRIMER_PAIR_521_PENALTY=8.388982 PRIMER_LEFT_521_PENALTY=4.293610 PRIMER_RIGHT_521_PENALTY=4.095371 PRIMER_LEFT_521_SEQUENCE=TGATTCCCTACCGCTGCTTAGT PRIMER_RIGHT_521_SEQUENCE=GGTGTAAGAATTTGCACTTGTCAG PRIMER_LEFT_521=534,22 PRIMER_RIGHT_521=611,24 PRIMER_LEFT_521_TM=62.294 PRIMER_RIGHT_521_TM=60.095 PRIMER_LEFT_521_GC_PERCENT=50.000 PRIMER_RIGHT_521_GC_PERCENT=41.667 PRIMER_LEFT_521_SELF_ANY=3.00 PRIMER_RIGHT_521_SELF_ANY=5.00 PRIMER_LEFT_521_SELF_END=1.00 PRIMER_RIGHT_521_SELF_END=1.00 PRIMER_LEFT_521_END_STABILITY=5.7000 PRIMER_RIGHT_521_END_STABILITY=6.4000 PRIMER_PAIR_521_COMPL_ANY=4.00 PRIMER_PAIR_521_COMPL_END=3.00 PRIMER_PAIR_521_PRODUCT_SIZE=78 PRIMER_PAIR_522_PENALTY=8.394044 PRIMER_LEFT_522_PENALTY=3.813742 PRIMER_RIGHT_522_PENALTY=4.580302 PRIMER_LEFT_522_SEQUENCE=GTGAGTTTGTAAGTGATGCCC PRIMER_RIGHT_522_SEQUENCE=AGTTTCGCAAACATCCATCCTC PRIMER_LEFT_522=558,21 PRIMER_RIGHT_522=637,22 PRIMER_LEFT_522_TM=57.186 PRIMER_RIGHT_522_TM=62.580 PRIMER_LEFT_522_GC_PERCENT=47.619 PRIMER_RIGHT_522_GC_PERCENT=45.455 PRIMER_LEFT_522_SELF_ANY=2.00 PRIMER_RIGHT_522_SELF_ANY=6.00 PRIMER_LEFT_522_SELF_END=0.00 PRIMER_RIGHT_522_SELF_END=0.00 PRIMER_LEFT_522_END_STABILITY=11.2000 PRIMER_RIGHT_522_END_STABILITY=7.9000 PRIMER_PAIR_522_COMPL_ANY=5.00 PRIMER_PAIR_522_COMPL_END=2.00 PRIMER_PAIR_522_PRODUCT_SIZE=80 PRIMER_PAIR_523_PENALTY=8.394044 PRIMER_LEFT_523_PENALTY=3.813742 PRIMER_RIGHT_523_PENALTY=4.580302 PRIMER_LEFT_523_SEQUENCE=GGTGAGTTTGTAAGTGATGCC PRIMER_RIGHT_523_SEQUENCE=GAGTTTCGCAAACATCCATCCT PRIMER_LEFT_523=557,21 PRIMER_RIGHT_523=638,22 PRIMER_LEFT_523_TM=57.186 PRIMER_RIGHT_523_TM=62.580 PRIMER_LEFT_523_GC_PERCENT=47.619 PRIMER_RIGHT_523_GC_PERCENT=45.455 PRIMER_LEFT_523_SELF_ANY=2.00 PRIMER_RIGHT_523_SELF_ANY=6.00 PRIMER_LEFT_523_SELF_END=1.00 PRIMER_RIGHT_523_SELF_END=0.00 PRIMER_LEFT_523_END_STABILITY=9.6000 PRIMER_RIGHT_523_END_STABILITY=7.8000 PRIMER_PAIR_523_COMPL_ANY=5.00 PRIMER_PAIR_523_COMPL_END=2.00 PRIMER_PAIR_523_PRODUCT_SIZE=82 PRIMER_PAIR_524_PENALTY=8.397256 PRIMER_LEFT_524_PENALTY=3.597672 PRIMER_RIGHT_524_PENALTY=4.799584 PRIMER_LEFT_524_SEQUENCE=CCAGGAGAGGATGGATGTT PRIMER_RIGHT_524_SEQUENCE=TGCAAGTTGGTACTCTTCTCACT PRIMER_LEFT_524=610,19 PRIMER_RIGHT_524=696,23 PRIMER_LEFT_524_TM=57.402 PRIMER_RIGHT_524_TM=58.200 PRIMER_LEFT_524_GC_PERCENT=52.632 PRIMER_RIGHT_524_GC_PERCENT=43.478 PRIMER_LEFT_524_SELF_ANY=3.00 PRIMER_RIGHT_524_SELF_ANY=5.00 PRIMER_LEFT_524_SELF_END=0.00 PRIMER_RIGHT_524_SELF_END=1.00 PRIMER_LEFT_524_END_STABILITY=6.6000 PRIMER_RIGHT_524_END_STABILITY=6.4000 PRIMER_PAIR_524_COMPL_ANY=5.00 PRIMER_PAIR_524_COMPL_END=1.00 PRIMER_PAIR_524_PRODUCT_SIZE=87 PRIMER_PAIR_525_PENALTY=8.402747 PRIMER_LEFT_525_PENALTY=2.395059 PRIMER_RIGHT_525_PENALTY=6.007688 PRIMER_LEFT_525_SEQUENCE=TCAGGGACCAAAACCTGC PRIMER_RIGHT_525_SEQUENCE=ATCTGCAGTTCAGGGTAGACTTCTT PRIMER_LEFT_525=368,18 PRIMER_RIGHT_525=444,25 PRIMER_LEFT_525_TM=59.605 PRIMER_RIGHT_525_TM=61.008 PRIMER_LEFT_525_GC_PERCENT=55.556 PRIMER_RIGHT_525_GC_PERCENT=44.000 PRIMER_LEFT_525_SELF_ANY=4.00 PRIMER_RIGHT_525_SELF_ANY=6.00 PRIMER_LEFT_525_SELF_END=2.00 PRIMER_RIGHT_525_SELF_END=2.00 PRIMER_LEFT_525_END_STABILITY=9.7000 PRIMER_RIGHT_525_END_STABILITY=7.0000 PRIMER_PAIR_525_COMPL_ANY=4.00 PRIMER_PAIR_525_COMPL_END=1.00 PRIMER_PAIR_525_PRODUCT_SIZE=77 PRIMER_PAIR_526_PENALTY=8.452406 PRIMER_LEFT_526_PENALTY=4.353331 PRIMER_RIGHT_526_PENALTY=4.099075 PRIMER_LEFT_526_SEQUENCE=GCTGCTTAGTTGGTGAGTTTGTA PRIMER_RIGHT_526_SEQUENCE=CATCCTCTCCTGGTGTAAGAATTT PRIMER_LEFT_526=546,23 PRIMER_RIGHT_526=622,24 PRIMER_LEFT_526_TM=58.647 PRIMER_RIGHT_526_TM=59.901 PRIMER_LEFT_526_GC_PERCENT=43.478 PRIMER_RIGHT_526_GC_PERCENT=41.667 PRIMER_LEFT_526_SELF_ANY=3.00 PRIMER_RIGHT_526_SELF_ANY=4.00 PRIMER_LEFT_526_SELF_END=2.00 PRIMER_RIGHT_526_SELF_END=3.00 PRIMER_LEFT_526_END_STABILITY=6.0000 PRIMER_RIGHT_526_END_STABILITY=7.2000 PRIMER_PAIR_526_COMPL_ANY=4.00 PRIMER_PAIR_526_COMPL_END=1.00 PRIMER_PAIR_526_PRODUCT_SIZE=77 PRIMER_PAIR_527_PENALTY=8.474577 PRIMER_LEFT_527_PENALTY=4.697688 PRIMER_RIGHT_527_PENALTY=3.776889 PRIMER_LEFT_527_SEQUENCE=TCACCAATGTGGTAGAAGCCAA PRIMER_RIGHT_527_SEQUENCE=GTCTTGCACTGCTTGCGG PRIMER_LEFT_527=444,22 PRIMER_RIGHT_527=519,18 PRIMER_LEFT_527_TM=62.698 PRIMER_RIGHT_527_TM=61.777 PRIMER_LEFT_527_GC_PERCENT=45.455 PRIMER_RIGHT_527_GC_PERCENT=61.111 PRIMER_LEFT_527_SELF_ANY=6.00 PRIMER_RIGHT_527_SELF_ANY=5.00 PRIMER_LEFT_527_SELF_END=0.00 PRIMER_RIGHT_527_SELF_END=2.00 PRIMER_LEFT_527_END_STABILITY=10.0000 PRIMER_RIGHT_527_END_STABILITY=11.7000 PRIMER_PAIR_527_COMPL_ANY=4.00 PRIMER_PAIR_527_COMPL_END=2.00 PRIMER_PAIR_527_PRODUCT_SIZE=76 PRIMER_PAIR_528_PENALTY=8.475039 PRIMER_LEFT_528_PENALTY=3.360461 PRIMER_RIGHT_528_PENALTY=5.114578 PRIMER_LEFT_528_SEQUENCE=CAAATTCTTACACCAGGAGAGGA PRIMER_RIGHT_528_SEQUENCE=CTCTTCTCACTGCATGTCTCTTT PRIMER_LEFT_528=598,23 PRIMER_RIGHT_528=684,23 PRIMER_LEFT_528_TM=59.640 PRIMER_RIGHT_528_TM=57.885 PRIMER_LEFT_528_GC_PERCENT=43.478 PRIMER_RIGHT_528_GC_PERCENT=43.478 PRIMER_LEFT_528_SELF_ANY=4.00 PRIMER_RIGHT_528_SELF_ANY=4.00 PRIMER_LEFT_528_SELF_END=3.00 PRIMER_RIGHT_528_SELF_END=0.00 PRIMER_LEFT_528_END_STABILITY=7.9000 PRIMER_RIGHT_528_END_STABILITY=7.0000 PRIMER_PAIR_528_COMPL_ANY=6.00 PRIMER_PAIR_528_COMPL_END=2.00 PRIMER_PAIR_528_PRODUCT_SIZE=87 PRIMER_PAIR_529_PENALTY=8.521362 PRIMER_LEFT_529_PENALTY=4.592761 PRIMER_RIGHT_529_PENALTY=3.928601 PRIMER_LEFT_529_SEQUENCE=ACTTTGTGATTCCCTACCGCTG PRIMER_RIGHT_529_SEQUENCE=AATTTGCACTTGTCAGGAACGA PRIMER_LEFT_529=528,22 PRIMER_RIGHT_529=603,22 PRIMER_LEFT_529_TM=62.593 PRIMER_RIGHT_529_TM=61.929 PRIMER_LEFT_529_GC_PERCENT=50.000 PRIMER_RIGHT_529_GC_PERCENT=40.909 PRIMER_LEFT_529_SELF_ANY=3.00 PRIMER_RIGHT_529_SELF_ANY=4.00 PRIMER_LEFT_529_SELF_END=2.00 PRIMER_RIGHT_529_SELF_END=1.00 PRIMER_LEFT_529_END_STABILITY=10.2000 PRIMER_RIGHT_529_END_STABILITY=8.4000 PRIMER_PAIR_529_COMPL_ANY=4.00 PRIMER_PAIR_529_COMPL_END=2.00 PRIMER_PAIR_529_PRODUCT_SIZE=76 PRIMER_PAIR_530_PENALTY=8.521362 PRIMER_LEFT_530_PENALTY=4.592761 PRIMER_RIGHT_530_PENALTY=3.928601 PRIMER_LEFT_530_SEQUENCE=CACTTTGTGATTCCCTACCGCT PRIMER_RIGHT_530_SEQUENCE=GAATTTGCACTTGTCAGGAACG PRIMER_LEFT_530=527,22 PRIMER_RIGHT_530=604,22 PRIMER_LEFT_530_TM=62.593 PRIMER_RIGHT_530_TM=61.929 PRIMER_LEFT_530_GC_PERCENT=50.000 PRIMER_RIGHT_530_GC_PERCENT=45.455 PRIMER_LEFT_530_SELF_ANY=3.00 PRIMER_RIGHT_530_SELF_ANY=4.00 PRIMER_LEFT_530_SELF_END=2.00 PRIMER_RIGHT_530_SELF_END=2.00 PRIMER_LEFT_530_END_STABILITY=11.4000 PRIMER_RIGHT_530_END_STABILITY=8.4000 PRIMER_PAIR_530_COMPL_ANY=4.00 PRIMER_PAIR_530_COMPL_END=2.00 PRIMER_PAIR_530_PRODUCT_SIZE=78 PRIMER_PAIR_531_PENALTY=8.543670 PRIMER_LEFT_531_PENALTY=4.411925 PRIMER_RIGHT_531_PENALTY=4.131746 PRIMER_LEFT_531_SEQUENCE=ACCGCTGCTTAGTTGGTGAGTT PRIMER_RIGHT_531_SEQUENCE=CCTCTCCTGGTGTAAGAATTTG PRIMER_LEFT_531=543,22 PRIMER_RIGHT_531=619,22 PRIMER_LEFT_531_TM=62.412 PRIMER_RIGHT_531_TM=57.868 PRIMER_LEFT_531_GC_PERCENT=50.000 PRIMER_RIGHT_531_GC_PERCENT=45.455 PRIMER_LEFT_531_SELF_ANY=3.00 PRIMER_RIGHT_531_SELF_ANY=4.00 PRIMER_LEFT_531_SELF_END=0.00 PRIMER_RIGHT_531_SELF_END=1.00 PRIMER_LEFT_531_END_STABILITY=6.4000 PRIMER_RIGHT_531_END_STABILITY=7.2000 PRIMER_PAIR_531_COMPL_ANY=4.00 PRIMER_PAIR_531_COMPL_END=0.00 PRIMER_PAIR_531_PRODUCT_SIZE=77 PRIMER_PAIR_532_PENALTY=8.575486 PRIMER_LEFT_532_PENALTY=3.718176 PRIMER_RIGHT_532_PENALTY=4.857310 PRIMER_LEFT_532_SEQUENCE=ATTCTTACACCAGGAGAGGATGG PRIMER_RIGHT_532_SEQUENCE=TGCAAGTTGGTACTCTTCTCAC PRIMER_LEFT_532=601,23 PRIMER_RIGHT_532=696,22 PRIMER_LEFT_532_TM=60.718 PRIMER_RIGHT_532_TM=57.143 PRIMER_LEFT_532_GC_PERCENT=47.826 PRIMER_RIGHT_532_GC_PERCENT=45.455 PRIMER_LEFT_532_SELF_ANY=3.00 PRIMER_RIGHT_532_SELF_ANY=5.00 PRIMER_LEFT_532_SELF_END=0.00 PRIMER_RIGHT_532_SELF_END=0.00 PRIMER_LEFT_532_END_STABILITY=8.1000 PRIMER_RIGHT_532_END_STABILITY=6.4000 PRIMER_PAIR_532_COMPL_ANY=5.00 PRIMER_PAIR_532_COMPL_END=2.00 PRIMER_PAIR_532_PRODUCT_SIZE=96 PRIMER_PAIR_533_PENALTY=8.613736 PRIMER_LEFT_533_PENALTY=3.380697 PRIMER_RIGHT_533_PENALTY=5.233039 PRIMER_LEFT_533_SEQUENCE=TTTGCGAAACTCATCTTCACT PRIMER_RIGHT_533_SEQUENCE=AGTCATGCAAGTTGGTACTCTTCTC PRIMER_LEFT_533=627,21 PRIMER_RIGHT_533=701,25 PRIMER_LEFT_533_TM=57.619 PRIMER_RIGHT_533_TM=60.233 PRIMER_LEFT_533_GC_PERCENT=38.095 PRIMER_RIGHT_533_GC_PERCENT=44.000 PRIMER_LEFT_533_SELF_ANY=4.00 PRIMER_RIGHT_533_SELF_ANY=5.00 PRIMER_LEFT_533_SELF_END=1.00 PRIMER_RIGHT_533_SELF_END=0.00 PRIMER_LEFT_533_END_STABILITY=6.4000 PRIMER_RIGHT_533_END_STABILITY=6.7000 PRIMER_PAIR_533_COMPL_ANY=4.00 PRIMER_PAIR_533_COMPL_END=1.00 PRIMER_PAIR_533_PRODUCT_SIZE=75 PRIMER_PAIR_534_PENALTY=8.649974 PRIMER_LEFT_534_PENALTY=6.202271 PRIMER_RIGHT_534_PENALTY=2.447703 PRIMER_LEFT_534_SEQUENCE=AAAGAGACATGCAGTGAGAAGAGTA PRIMER_RIGHT_534_SEQUENCE=AACACACAAACTCTACCCCTCG PRIMER_LEFT_534=662,25 PRIMER_RIGHT_534=758,22 PRIMER_LEFT_534_TM=58.798 PRIMER_RIGHT_534_TM=60.448 PRIMER_LEFT_534_GC_PERCENT=40.000 PRIMER_RIGHT_534_GC_PERCENT=50.000 PRIMER_LEFT_534_SELF_ANY=4.00 PRIMER_RIGHT_534_SELF_ANY=2.00 PRIMER_LEFT_534_SELF_END=2.00 PRIMER_RIGHT_534_SELF_END=2.00 PRIMER_LEFT_534_END_STABILITY=5.4000 PRIMER_RIGHT_534_END_STABILITY=9.9000 PRIMER_PAIR_534_COMPL_ANY=5.00 PRIMER_PAIR_534_COMPL_END=3.00 PRIMER_PAIR_534_PRODUCT_SIZE=97 PRIMER_PAIR_535_PENALTY=8.694212 PRIMER_LEFT_535_PENALTY=4.985387 PRIMER_RIGHT_535_PENALTY=3.708825 PRIMER_LEFT_535_SEQUENCE=GTGCAAGACCCATCCCCA PRIMER_RIGHT_535_SEQUENCE=GTCAGGAACGAGAAGGGC PRIMER_LEFT_535=511,18 PRIMER_RIGHT_535=592,18 PRIMER_LEFT_535_TM=62.985 PRIMER_RIGHT_535_TM=58.291 PRIMER_LEFT_535_GC_PERCENT=61.111 PRIMER_RIGHT_535_GC_PERCENT=61.111 PRIMER_LEFT_535_SELF_ANY=4.00 PRIMER_RIGHT_535_SELF_ANY=2.00 PRIMER_LEFT_535_SELF_END=0.00 PRIMER_RIGHT_535_SELF_END=2.00 PRIMER_LEFT_535_END_STABILITY=11.2000 PRIMER_RIGHT_535_END_STABILITY=10.9000 PRIMER_PAIR_535_COMPL_ANY=4.00 PRIMER_PAIR_535_COMPL_END=1.00 PRIMER_PAIR_535_PRODUCT_SIZE=82 PRIMER_PAIR_536_PENALTY=8.696953 PRIMER_LEFT_536_PENALTY=4.792901 PRIMER_RIGHT_536_PENALTY=3.904052 PRIMER_LEFT_536_SEQUENCE=CAGATCACCAATGTGGTAGAAG PRIMER_RIGHT_536_SEQUENCE=ACAAAGTGGGGATGGGTC PRIMER_LEFT_536=440,22 PRIMER_RIGHT_536=534,18 PRIMER_LEFT_536_TM=57.207 PRIMER_RIGHT_536_TM=58.096 PRIMER_LEFT_536_GC_PERCENT=45.455 PRIMER_RIGHT_536_GC_PERCENT=55.556 PRIMER_LEFT_536_SELF_ANY=6.00 PRIMER_RIGHT_536_SELF_ANY=2.00 PRIMER_LEFT_536_SELF_END=2.00 PRIMER_RIGHT_536_SELF_END=1.00 PRIMER_LEFT_536_END_STABILITY=6.7000 PRIMER_RIGHT_536_END_STABILITY=9.1000 PRIMER_PAIR_536_COMPL_ANY=6.00 PRIMER_PAIR_536_COMPL_END=1.00 PRIMER_PAIR_536_PRODUCT_SIZE=95 PRIMER_PAIR_537_PENALTY=8.711063 PRIMER_LEFT_537_PENALTY=4.187633 PRIMER_RIGHT_537_PENALTY=4.523430 PRIMER_LEFT_537_SEQUENCE=TTGGTGAGTTTGTAAGTGATGC PRIMER_RIGHT_537_SEQUENCE=AAACATCCATCCTCTCCTGGTG PRIMER_LEFT_537=555,22 PRIMER_RIGHT_537=629,22 PRIMER_LEFT_537_TM=57.812 PRIMER_RIGHT_537_TM=62.523 PRIMER_LEFT_537_GC_PERCENT=40.909 PRIMER_RIGHT_537_GC_PERCENT=50.000 PRIMER_LEFT_537_SELF_ANY=2.00 PRIMER_RIGHT_537_SELF_ANY=3.00 PRIMER_LEFT_537_SELF_END=2.00 PRIMER_RIGHT_537_SELF_END=3.00 PRIMER_LEFT_537_END_STABILITY=8.1000 PRIMER_RIGHT_537_END_STABILITY=8.2000 PRIMER_PAIR_537_COMPL_ANY=5.00 PRIMER_PAIR_537_COMPL_END=1.00 PRIMER_PAIR_537_PRODUCT_SIZE=75 PRIMER_PAIR_538_PENALTY=8.723994 PRIMER_LEFT_538_PENALTY=3.536297 PRIMER_RIGHT_538_PENALTY=5.187697 PRIMER_LEFT_538_SEQUENCE=ATGTTTGCGAAACTCATCTTCAC PRIMER_RIGHT_538_SEQUENCE=TCATGCAAGTTGGTACTCTTCTCA PRIMER_LEFT_538=624,23 PRIMER_RIGHT_538=699,24 PRIMER_LEFT_538_TM=60.536 PRIMER_RIGHT_538_TM=61.188 PRIMER_LEFT_538_GC_PERCENT=39.130 PRIMER_RIGHT_538_GC_PERCENT=41.667 PRIMER_LEFT_538_SELF_ANY=6.00 PRIMER_RIGHT_538_SELF_ANY=5.00 PRIMER_LEFT_538_SELF_END=3.00 PRIMER_RIGHT_538_SELF_END=1.00 PRIMER_LEFT_538_END_STABILITY=6.7000 PRIMER_RIGHT_538_END_STABILITY=6.7000 PRIMER_PAIR_538_COMPL_ANY=4.00 PRIMER_PAIR_538_COMPL_END=2.00 PRIMER_PAIR_538_PRODUCT_SIZE=76 PRIMER_PAIR_539_PENALTY=8.784018 PRIMER_LEFT_539_PENALTY=3.527826 PRIMER_RIGHT_539_PENALTY=5.256192 PRIMER_LEFT_539_SEQUENCE=GTGCAAATTCTTACACCAGGAGA PRIMER_RIGHT_539_SEQUENCE=ACTCTTCTCACTGCATGTCTCTTT PRIMER_LEFT_539=595,23 PRIMER_RIGHT_539=685,24 PRIMER_LEFT_539_TM=60.528 PRIMER_RIGHT_539_TM=58.744 PRIMER_LEFT_539_GC_PERCENT=43.478 PRIMER_RIGHT_539_GC_PERCENT=41.667 PRIMER_LEFT_539_SELF_ANY=5.00 PRIMER_RIGHT_539_SELF_ANY=4.00 PRIMER_LEFT_539_SELF_END=1.00 PRIMER_RIGHT_539_SELF_END=0.00 PRIMER_LEFT_539_END_STABILITY=7.9000 PRIMER_RIGHT_539_END_STABILITY=7.0000 PRIMER_PAIR_539_COMPL_ANY=5.00 PRIMER_PAIR_539_COMPL_END=3.00 PRIMER_PAIR_539_PRODUCT_SIZE=91 PRIMER_PAIR_540_PENALTY=8.811095 PRIMER_LEFT_540_PENALTY=4.594300 PRIMER_RIGHT_540_PENALTY=4.216794 PRIMER_LEFT_540_SEQUENCE=ACCGCTGCTTAGTTGGTG PRIMER_RIGHT_540_SEQUENCE=ATCCATCCTCTCCTGGTGTAAGA PRIMER_LEFT_540=543,18 PRIMER_RIGHT_540=625,23 PRIMER_LEFT_540_TM=57.406 PRIMER_RIGHT_540_TM=61.217 PRIMER_LEFT_540_GC_PERCENT=55.556 PRIMER_RIGHT_540_GC_PERCENT=47.826 PRIMER_LEFT_540_SELF_ANY=3.00 PRIMER_RIGHT_540_SELF_ANY=3.00 PRIMER_LEFT_540_SELF_END=0.00 PRIMER_RIGHT_540_SELF_END=3.00 PRIMER_LEFT_540_END_STABILITY=8.2000 PRIMER_RIGHT_540_END_STABILITY=6.0000 PRIMER_PAIR_540_COMPL_ANY=4.00 PRIMER_PAIR_540_COMPL_END=0.00 PRIMER_PAIR_540_PRODUCT_SIZE=83 PRIMER_PAIR_541_PENALTY=8.816618 PRIMER_LEFT_541_PENALTY=4.293188 PRIMER_RIGHT_541_PENALTY=4.523430 PRIMER_LEFT_541_SEQUENCE=TTGGTGAGTTTGTAAGTGATGCC PRIMER_RIGHT_541_SEQUENCE=CAAACATCCATCCTCTCCTGGT PRIMER_LEFT_541=555,23 PRIMER_RIGHT_541=630,22 PRIMER_LEFT_541_TM=61.293 PRIMER_RIGHT_541_TM=62.523 PRIMER_LEFT_541_GC_PERCENT=43.478 PRIMER_RIGHT_541_GC_PERCENT=50.000 PRIMER_LEFT_541_SELF_ANY=2.00 PRIMER_RIGHT_541_SELF_ANY=3.00 PRIMER_LEFT_541_SELF_END=1.00 PRIMER_RIGHT_541_SELF_END=1.00 PRIMER_LEFT_541_END_STABILITY=9.6000 PRIMER_RIGHT_541_END_STABILITY=7.9000 PRIMER_PAIR_541_COMPL_ANY=5.00 PRIMER_PAIR_541_COMPL_END=1.00 PRIMER_PAIR_541_PRODUCT_SIZE=76 PRIMER_PAIR_542_PENALTY=8.836638 PRIMER_LEFT_542_PENALTY=3.968084 PRIMER_RIGHT_542_PENALTY=4.868554 PRIMER_LEFT_542_SEQUENCE=GAGGATGGATGTTTGCGA PRIMER_RIGHT_542_SEQUENCE=TGCAAGTTGGTACTCTTCTCACTG PRIMER_LEFT_542=616,18 PRIMER_RIGHT_542=696,24 PRIMER_LEFT_542_TM=58.032 PRIMER_RIGHT_542_TM=60.869 PRIMER_LEFT_542_GC_PERCENT=50.000 PRIMER_RIGHT_542_GC_PERCENT=45.833 PRIMER_LEFT_542_SELF_ANY=2.00 PRIMER_RIGHT_542_SELF_ANY=5.00 PRIMER_LEFT_542_SELF_END=2.00 PRIMER_RIGHT_542_SELF_END=3.00 PRIMER_LEFT_542_END_STABILITY=10.2000 PRIMER_RIGHT_542_END_STABILITY=6.7000 PRIMER_PAIR_542_COMPL_ANY=4.00 PRIMER_PAIR_542_COMPL_END=0.00 PRIMER_PAIR_542_PRODUCT_SIZE=81 PRIMER_PAIR_543_PENALTY=8.852178 PRIMER_LEFT_543_PENALTY=5.351477 PRIMER_RIGHT_543_PENALTY=3.500701 PRIMER_LEFT_543_SEQUENCE=CTGGAGGTACCCACTGATGGTAA PRIMER_RIGHT_543_SEQUENCE=ACATTCATGTGCATGTTCAGTC PRIMER_LEFT_543=251,23 PRIMER_RIGHT_543=339,22 PRIMER_LEFT_543_TM=62.351 PRIMER_RIGHT_543_TM=58.499 PRIMER_LEFT_543_GC_PERCENT=52.174 PRIMER_RIGHT_543_GC_PERCENT=40.909 PRIMER_LEFT_543_SELF_ANY=6.00 PRIMER_RIGHT_543_SELF_ANY=8.00 PRIMER_LEFT_543_SELF_END=3.00 PRIMER_RIGHT_543_SELF_END=1.00 PRIMER_LEFT_543_END_STABILITY=7.2000 PRIMER_RIGHT_543_END_STABILITY=6.4000 PRIMER_PAIR_543_COMPL_ANY=5.00 PRIMER_PAIR_543_COMPL_END=1.00 PRIMER_PAIR_543_PRODUCT_SIZE=89 PRIMER_PAIR_544_PENALTY=8.929890 PRIMER_LEFT_544_PENALTY=3.487465 PRIMER_RIGHT_544_PENALTY=5.442425 PRIMER_LEFT_544_SEQUENCE=GTTTGCGAAACTCATCTTCACT PRIMER_RIGHT_544_SEQUENCE=GTCATGCAAGTTGGTACTCTTCT PRIMER_LEFT_544=626,22 PRIMER_RIGHT_544=700,23 PRIMER_LEFT_544_TM=58.513 PRIMER_RIGHT_544_TM=57.558 PRIMER_LEFT_544_GC_PERCENT=40.909 PRIMER_RIGHT_544_GC_PERCENT=43.478 PRIMER_LEFT_544_SELF_ANY=6.00 PRIMER_RIGHT_544_SELF_ANY=5.00 PRIMER_LEFT_544_SELF_END=1.00 PRIMER_RIGHT_544_SELF_END=2.00 PRIMER_LEFT_544_END_STABILITY=6.4000 PRIMER_RIGHT_544_END_STABILITY=6.7000 PRIMER_PAIR_544_COMPL_ANY=4.00 PRIMER_PAIR_544_COMPL_END=0.00 PRIMER_PAIR_544_PRODUCT_SIZE=75 PRIMER_PAIR_545_PENALTY=8.956596 PRIMER_LEFT_545_PENALTY=4.590174 PRIMER_RIGHT_545_PENALTY=4.366422 PRIMER_LEFT_545_SEQUENCE=GGACCAAAACCTGCATTG PRIMER_RIGHT_545_SEQUENCE=GGTTGGTTGGCTTCTACCACAT PRIMER_LEFT_545=372,18 PRIMER_RIGHT_545=471,22 PRIMER_LEFT_545_TM=57.410 PRIMER_RIGHT_545_TM=62.366 PRIMER_LEFT_545_GC_PERCENT=50.000 PRIMER_RIGHT_545_GC_PERCENT=50.000 PRIMER_LEFT_545_SELF_ANY=4.00 PRIMER_RIGHT_545_SELF_ANY=4.00 PRIMER_LEFT_545_SELF_END=3.00 PRIMER_RIGHT_545_SELF_END=2.00 PRIMER_LEFT_545_END_STABILITY=7.2000 PRIMER_RIGHT_545_END_STABILITY=6.6000 PRIMER_PAIR_545_COMPL_ANY=5.00 PRIMER_PAIR_545_COMPL_END=2.00 PRIMER_PAIR_545_PRODUCT_SIZE=100 PRIMER_PAIR_546_PENALTY=8.977719 PRIMER_LEFT_546_PENALTY=3.535294 PRIMER_RIGHT_546_PENALTY=5.442425 PRIMER_LEFT_546_SEQUENCE=GTTTGCGAAACTCATCTTCAC PRIMER_RIGHT_546_SEQUENCE=AGTCATGCAAGTTGGTACTCTTC PRIMER_LEFT_546=626,21 PRIMER_RIGHT_546=701,23 PRIMER_LEFT_546_TM=57.465 PRIMER_RIGHT_546_TM=57.558 PRIMER_LEFT_546_GC_PERCENT=42.857 PRIMER_RIGHT_546_GC_PERCENT=43.478 PRIMER_LEFT_546_SELF_ANY=6.00 PRIMER_RIGHT_546_SELF_ANY=5.00 PRIMER_LEFT_546_SELF_END=3.00 PRIMER_RIGHT_546_SELF_END=0.00 PRIMER_LEFT_546_END_STABILITY=6.7000 PRIMER_RIGHT_546_END_STABILITY=6.7000 PRIMER_PAIR_546_COMPL_ANY=4.00 PRIMER_PAIR_546_COMPL_END=1.00 PRIMER_PAIR_546_PRODUCT_SIZE=76 PRIMER_PAIR_547_PENALTY=8.992907 PRIMER_LEFT_547_PENALTY=5.831817 PRIMER_RIGHT_547_PENALTY=3.161089 PRIMER_LEFT_547_SEQUENCE=TCCTGCAGTATTGCCAAGAAGTC PRIMER_RIGHT_547_SEQUENCE=TGGTCACTGGTTGGTTGG PRIMER_LEFT_547=405,23 PRIMER_RIGHT_547=479,18 PRIMER_LEFT_547_TM=62.832 PRIMER_RIGHT_547_TM=58.839 PRIMER_LEFT_547_GC_PERCENT=47.826 PRIMER_RIGHT_547_GC_PERCENT=55.556 PRIMER_LEFT_547_SELF_ANY=6.00 PRIMER_RIGHT_547_SELF_ANY=3.00 PRIMER_LEFT_547_SELF_END=2.00 PRIMER_RIGHT_547_SELF_END=0.00 PRIMER_LEFT_547_END_STABILITY=6.4000 PRIMER_RIGHT_547_END_STABILITY=8.2000 PRIMER_PAIR_547_COMPL_ANY=5.00 PRIMER_PAIR_547_COMPL_END=2.00 PRIMER_PAIR_547_PRODUCT_SIZE=75 PRIMER_PAIR_548_PENALTY=9.029769 PRIMER_LEFT_548_PENALTY=3.647215 PRIMER_RIGHT_548_PENALTY=5.382554 PRIMER_LEFT_548_SEQUENCE=TGCAAATTCTTACACCAGGAGAG PRIMER_RIGHT_548_SEQUENCE=GGTACTCTTCTCACTGCATGTCT PRIMER_LEFT_548=596,23 PRIMER_RIGHT_548=688,23 PRIMER_LEFT_548_TM=60.647 PRIMER_RIGHT_548_TM=57.617 PRIMER_LEFT_548_GC_PERCENT=43.478 PRIMER_RIGHT_548_GC_PERCENT=47.826 PRIMER_LEFT_548_SELF_ANY=4.00 PRIMER_RIGHT_548_SELF_ANY=4.00 PRIMER_LEFT_548_SELF_END=0.00 PRIMER_RIGHT_548_SELF_END=0.00 PRIMER_LEFT_548_END_STABILITY=6.4000 PRIMER_RIGHT_548_END_STABILITY=6.4000 PRIMER_PAIR_548_COMPL_ANY=5.00 PRIMER_PAIR_548_COMPL_END=2.00 PRIMER_PAIR_548_PRODUCT_SIZE=93 PRIMER_PAIR_549_PENALTY=9.051676 PRIMER_LEFT_549_PENALTY=3.686405 PRIMER_RIGHT_549_PENALTY=5.365271 PRIMER_LEFT_549_SEQUENCE=AAGTGCAAATTCTTACACCAGG PRIMER_RIGHT_549_SEQUENCE=GGTACTCTTCTCACTGCATGTCTCT PRIMER_LEFT_549=593,22 PRIMER_RIGHT_549=688,25 PRIMER_LEFT_549_TM=58.314 PRIMER_RIGHT_549_TM=60.365 PRIMER_LEFT_549_GC_PERCENT=40.909 PRIMER_RIGHT_549_GC_PERCENT=48.000 PRIMER_LEFT_549_SELF_ANY=5.00 PRIMER_RIGHT_549_SELF_ANY=4.00 PRIMER_LEFT_549_SELF_END=3.00 PRIMER_RIGHT_549_SELF_END=0.00 PRIMER_LEFT_549_END_STABILITY=9.7000 PRIMER_RIGHT_549_END_STABILITY=6.4000 PRIMER_PAIR_549_COMPL_ANY=4.00 PRIMER_PAIR_549_COMPL_END=1.00 PRIMER_PAIR_549_PRODUCT_SIZE=96 PRIMER_PAIR_550_PENALTY=9.061120 PRIMER_LEFT_550_PENALTY=6.336480 PRIMER_RIGHT_550_PENALTY=2.724641 PRIMER_LEFT_550_SEQUENCE=CAAAGAGACATGCAGTGAGAAGAGT PRIMER_RIGHT_550_SEQUENCE=AAACTCTACCCCTCGGAACTT PRIMER_LEFT_550=661,25 PRIMER_RIGHT_550=751,21 PRIMER_LEFT_550_TM=61.336 PRIMER_RIGHT_550_TM=58.275 PRIMER_LEFT_550_GC_PERCENT=44.000 PRIMER_RIGHT_550_GC_PERCENT=47.619 PRIMER_LEFT_550_SELF_ANY=4.00 PRIMER_RIGHT_550_SELF_ANY=3.00 PRIMER_LEFT_550_SELF_END=1.00 PRIMER_RIGHT_550_SELF_END=3.00 PRIMER_LEFT_550_END_STABILITY=6.1000 PRIMER_RIGHT_550_END_STABILITY=6.7000 PRIMER_PAIR_550_COMPL_ANY=5.00 PRIMER_PAIR_550_COMPL_END=2.00 PRIMER_PAIR_550_PRODUCT_SIZE=91 PRIMER_PAIR_551_PENALTY=9.062113 PRIMER_LEFT_551_PENALTY=2.423358 PRIMER_RIGHT_551_PENALTY=6.638755 PRIMER_LEFT_551_SEQUENCE=TCCAGAATGGGAAGTGGGA PRIMER_RIGHT_551_SEQUENCE=TCAGGGTAGACTTCTTGGCAATACT PRIMER_LEFT_551=339,19 PRIMER_RIGHT_551=435,25 PRIMER_LEFT_551_TM=61.423 PRIMER_RIGHT_551_TM=61.639 PRIMER_LEFT_551_GC_PERCENT=52.632 PRIMER_RIGHT_551_GC_PERCENT=44.000 PRIMER_LEFT_551_SELF_ANY=3.00 PRIMER_RIGHT_551_SELF_ANY=5.00 PRIMER_LEFT_551_SELF_END=0.00 PRIMER_RIGHT_551_SELF_END=2.00 PRIMER_LEFT_551_END_STABILITY=9.7000 PRIMER_RIGHT_551_END_STABILITY=5.3000 PRIMER_PAIR_551_COMPL_ANY=5.00 PRIMER_PAIR_551_COMPL_END=1.00 PRIMER_PAIR_551_PRODUCT_SIZE=97 PRIMER_PAIR_552_PENALTY=9.078622 PRIMER_LEFT_552_PENALTY=4.983250 PRIMER_RIGHT_552_PENALTY=4.095371 PRIMER_LEFT_552_SEQUENCE=TTTGTGATTCCCTACCGC PRIMER_RIGHT_552_SEQUENCE=CTGGTGTAAGAATTTGCACTTGTC PRIMER_LEFT_552=530,18 PRIMER_RIGHT_552=613,24 PRIMER_LEFT_552_TM=57.017 PRIMER_RIGHT_552_TM=60.095 PRIMER_LEFT_552_GC_PERCENT=50.000 PRIMER_RIGHT_552_GC_PERCENT=41.667 PRIMER_LEFT_552_SELF_ANY=3.00 PRIMER_RIGHT_552_SELF_ANY=5.00 PRIMER_LEFT_552_SELF_END=2.00 PRIMER_RIGHT_552_SELF_END=2.00 PRIMER_LEFT_552_END_STABILITY=11.1000 PRIMER_RIGHT_552_END_STABILITY=6.7000 PRIMER_PAIR_552_COMPL_ANY=4.00 PRIMER_PAIR_552_COMPL_END=0.00 PRIMER_PAIR_552_PRODUCT_SIZE=84 PRIMER_PAIR_553_PENALTY=9.144420 PRIMER_LEFT_553_PENALTY=5.925828 PRIMER_RIGHT_553_PENALTY=3.218591 PRIMER_LEFT_553_SEQUENCE=CCTGCAGTATTGCCAAGAAGTCTA PRIMER_RIGHT_553_SEQUENCE=TGGATGGTCACTGGTTGG PRIMER_LEFT_553=406,24 PRIMER_RIGHT_553=483,18 PRIMER_LEFT_553_TM=61.926 PRIMER_RIGHT_553_TM=58.781 PRIMER_LEFT_553_GC_PERCENT=45.833 PRIMER_RIGHT_553_GC_PERCENT=55.556 PRIMER_LEFT_553_SELF_ANY=6.00 PRIMER_RIGHT_553_SELF_ANY=3.00 PRIMER_LEFT_553_SELF_END=2.00 PRIMER_RIGHT_553_SELF_END=0.00 PRIMER_LEFT_553_END_STABILITY=5.4000 PRIMER_RIGHT_553_END_STABILITY=8.2000 PRIMER_PAIR_553_COMPL_ANY=5.00 PRIMER_PAIR_553_COMPL_END=3.00 PRIMER_PAIR_553_PRODUCT_SIZE=78 PRIMER_PAIR_554_PENALTY=9.168577 PRIMER_LEFT_554_PENALTY=4.841851 PRIMER_RIGHT_554_PENALTY=4.326727 PRIMER_LEFT_554_SEQUENCE=CCGCTGCTTAGTTGGTGAGTTT PRIMER_RIGHT_554_SEQUENCE=CCTCTCCTGGTGTAAGAATTTGC PRIMER_LEFT_554=544,22 PRIMER_RIGHT_554=619,23 PRIMER_LEFT_554_TM=62.842 PRIMER_RIGHT_554_TM=61.327 PRIMER_LEFT_554_GC_PERCENT=50.000 PRIMER_RIGHT_554_GC_PERCENT=47.826 PRIMER_LEFT_554_SELF_ANY=3.00 PRIMER_RIGHT_554_SELF_ANY=4.00 PRIMER_LEFT_554_SELF_END=0.00 PRIMER_RIGHT_554_SELF_END=2.00 PRIMER_LEFT_554_END_STABILITY=6.7000 PRIMER_RIGHT_554_END_STABILITY=8.8000 PRIMER_PAIR_554_COMPL_ANY=4.00 PRIMER_PAIR_554_COMPL_END=0.00 PRIMER_PAIR_554_PRODUCT_SIZE=76 PRIMER_PAIR_555_PENALTY=9.177766 PRIMER_LEFT_555_PENALTY=3.234765 PRIMER_RIGHT_555_PENALTY=5.943001 PRIMER_LEFT_555_SEQUENCE=GCGAAACTCATCTTCACTGGC PRIMER_RIGHT_555_SEQUENCE=CGTAGTCATGCAAGTTGGTACTCTT PRIMER_LEFT_555=630,21 PRIMER_RIGHT_555=704,25 PRIMER_LEFT_555_TM=62.235 PRIMER_RIGHT_555_TM=60.943 PRIMER_LEFT_555_GC_PERCENT=52.381 PRIMER_RIGHT_555_GC_PERCENT=44.000 PRIMER_LEFT_555_SELF_ANY=3.00 PRIMER_RIGHT_555_SELF_ANY=5.00 PRIMER_LEFT_555_SELF_END=2.00 PRIMER_RIGHT_555_SELF_END=0.00 PRIMER_LEFT_555_END_STABILITY=9.7000 PRIMER_RIGHT_555_END_STABILITY=6.7000 PRIMER_PAIR_555_COMPL_ANY=4.00 PRIMER_PAIR_555_COMPL_END=2.00 PRIMER_PAIR_555_PRODUCT_SIZE=75 PRIMER_PAIR_556_PENALTY=9.184810 PRIMER_LEFT_556_PENALTY=3.532432 PRIMER_RIGHT_556_PENALTY=5.652377 PRIMER_LEFT_556_SEQUENCE=TCCTGACAAGTGCAAATTCTT PRIMER_RIGHT_556_SEQUENCE=ACTCTTCTCACTGCATGTCTCTT PRIMER_LEFT_556=586,21 PRIMER_RIGHT_556=685,23 PRIMER_LEFT_556_TM=57.468 PRIMER_RIGHT_556_TM=57.348 PRIMER_LEFT_556_GC_PERCENT=38.095 PRIMER_RIGHT_556_GC_PERCENT=43.478 PRIMER_LEFT_556_SELF_ANY=4.00 PRIMER_RIGHT_556_SELF_ANY=4.00 PRIMER_LEFT_556_SELF_END=1.00 PRIMER_RIGHT_556_SELF_END=0.00 PRIMER_LEFT_556_END_STABILITY=7.0000 PRIMER_RIGHT_556_END_STABILITY=6.7000 PRIMER_PAIR_556_COMPL_ANY=5.00 PRIMER_PAIR_556_COMPL_END=1.00 PRIMER_PAIR_556_PRODUCT_SIZE=100 PRIMER_PAIR_557_PENALTY=9.213735 PRIMER_LEFT_557_PENALTY=5.713033 PRIMER_RIGHT_557_PENALTY=3.500701 PRIMER_LEFT_557_SEQUENCE=GGTACCCACTGATGGTAATGCTG PRIMER_RIGHT_557_SEQUENCE=GACATTCATGTGCATGTTCAGT PRIMER_LEFT_557=256,23 PRIMER_RIGHT_557=340,22 PRIMER_LEFT_557_TM=62.713 PRIMER_RIGHT_557_TM=58.499 PRIMER_LEFT_557_GC_PERCENT=52.174 PRIMER_RIGHT_557_GC_PERCENT=40.909 PRIMER_LEFT_557_SELF_ANY=6.00 PRIMER_RIGHT_557_SELF_ANY=8.00 PRIMER_LEFT_557_SELF_END=1.00 PRIMER_RIGHT_557_SELF_END=2.00 PRIMER_LEFT_557_END_STABILITY=8.5000 PRIMER_RIGHT_557_END_STABILITY=6.4000 PRIMER_PAIR_557_COMPL_ANY=5.00 PRIMER_PAIR_557_COMPL_END=2.00 PRIMER_PAIR_557_PRODUCT_SIZE=85 PRIMER_PAIR_558_PENALTY=9.236373 PRIMER_LEFT_558_PENALTY=6.939511 PRIMER_RIGHT_558_PENALTY=2.296862 PRIMER_LEFT_558_SEQUENCE=ATCCTGCAGTATTGCCAAGAAGTC PRIMER_RIGHT_558_SEQUENCE=GGTCACTGGTTGGTTGGCTT PRIMER_LEFT_558=404,24 PRIMER_RIGHT_558=478,20 PRIMER_LEFT_558_TM=62.940 PRIMER_RIGHT_558_TM=62.297 PRIMER_LEFT_558_GC_PERCENT=45.833 PRIMER_RIGHT_558_GC_PERCENT=55.000 PRIMER_LEFT_558_SELF_ANY=8.00 PRIMER_RIGHT_558_SELF_ANY=3.00 PRIMER_LEFT_558_SELF_END=2.00 PRIMER_RIGHT_558_SELF_END=0.00 PRIMER_LEFT_558_END_STABILITY=6.4000 PRIMER_RIGHT_558_END_STABILITY=9.7000 PRIMER_PAIR_558_COMPL_ANY=5.00 PRIMER_PAIR_558_COMPL_END=3.00 PRIMER_PAIR_558_PRODUCT_SIZE=75 PRIMER_PAIR_559_PENALTY=9.244053 PRIMER_LEFT_559_PENALTY=4.310125 PRIMER_RIGHT_559_PENALTY=4.933928 PRIMER_LEFT_559_SEQUENCE=GTTGGTGAGTTTGTAAGTGATGC PRIMER_RIGHT_559_SEQUENCE=CGCAAACATCCATCCTCT PRIMER_LEFT_559=554,23 PRIMER_RIGHT_559=632,18 PRIMER_LEFT_559_TM=58.690 PRIMER_RIGHT_559_TM=57.066 PRIMER_LEFT_559_GC_PERCENT=43.478 PRIMER_RIGHT_559_GC_PERCENT=50.000 PRIMER_LEFT_559_SELF_ANY=2.00 PRIMER_RIGHT_559_SELF_ANY=2.00 PRIMER_LEFT_559_SELF_END=2.00 PRIMER_RIGHT_559_SELF_END=0.00 PRIMER_LEFT_559_END_STABILITY=8.1000 PRIMER_RIGHT_559_END_STABILITY=7.9000 PRIMER_PAIR_559_COMPL_ANY=5.00 PRIMER_PAIR_559_COMPL_END=3.00 PRIMER_PAIR_559_PRODUCT_SIZE=79 PRIMER_PAIR_560_PENALTY=9.287367 PRIMER_LEFT_560_PENALTY=3.579454 PRIMER_RIGHT_560_PENALTY=5.707913 PRIMER_LEFT_560_SEQUENCE=CTCGTTCCTGACAAGTGCAAAT PRIMER_RIGHT_560_SEQUENCE=GGTGTGCCAGTGAAGATGAGTTT PRIMER_LEFT_560=581,22 PRIMER_RIGHT_560=655,23 PRIMER_LEFT_560_TM=61.579 PRIMER_RIGHT_560_TM=62.708 PRIMER_LEFT_560_GC_PERCENT=45.455 PRIMER_RIGHT_560_GC_PERCENT=47.826 PRIMER_LEFT_560_SELF_ANY=4.00 PRIMER_RIGHT_560_SELF_ANY=3.00 PRIMER_LEFT_560_SELF_END=2.00 PRIMER_RIGHT_560_SELF_END=1.00 PRIMER_LEFT_560_END_STABILITY=7.2000 PRIMER_RIGHT_560_END_STABILITY=6.7000 PRIMER_PAIR_560_COMPL_ANY=5.00 PRIMER_PAIR_560_COMPL_END=2.00 PRIMER_PAIR_560_PRODUCT_SIZE=75 PRIMER_PAIR_561_PENALTY=9.365653 PRIMER_LEFT_561_PENALTY=4.370754 PRIMER_RIGHT_561_PENALTY=4.994899 PRIMER_LEFT_561_SEQUENCE=AGTTGGTGAGTTTGTAAGTGATGC PRIMER_RIGHT_561_SEQUENCE=ACATCCATCCTCTCCTGGTGTAA PRIMER_LEFT_561=553,24 PRIMER_RIGHT_561=627,23 PRIMER_LEFT_561_TM=59.629 PRIMER_RIGHT_561_TM=61.995 PRIMER_LEFT_561_GC_PERCENT=41.667 PRIMER_RIGHT_561_GC_PERCENT=47.826 PRIMER_LEFT_561_SELF_ANY=2.00 PRIMER_RIGHT_561_SELF_ANY=4.00 PRIMER_LEFT_561_SELF_END=2.00 PRIMER_RIGHT_561_SELF_END=1.00 PRIMER_LEFT_561_END_STABILITY=8.1000 PRIMER_RIGHT_561_END_STABILITY=6.0000 PRIMER_PAIR_561_COMPL_ANY=5.00 PRIMER_PAIR_561_COMPL_END=0.00 PRIMER_PAIR_561_PRODUCT_SIZE=75 PRIMER_PAIR_562_PENALTY=9.446797 PRIMER_LEFT_562_PENALTY=3.991211 PRIMER_RIGHT_562_PENALTY=5.455586 PRIMER_LEFT_562_SEQUENCE=GCAAATTCTTACACCAGGAGAG PRIMER_RIGHT_562_SEQUENCE=AAGTTGGTACTCTTCTCACTGCAT PRIMER_LEFT_562=597,22 PRIMER_RIGHT_562=693,24 PRIMER_LEFT_562_TM=58.009 PRIMER_RIGHT_562_TM=58.544 PRIMER_LEFT_562_GC_PERCENT=45.455 PRIMER_RIGHT_562_GC_PERCENT=41.667 PRIMER_LEFT_562_SELF_ANY=4.00 PRIMER_RIGHT_562_SELF_ANY=4.00 PRIMER_LEFT_562_SELF_END=0.00 PRIMER_RIGHT_562_SELF_END=2.00 PRIMER_LEFT_562_END_STABILITY=6.4000 PRIMER_RIGHT_562_END_STABILITY=8.4000 PRIMER_PAIR_562_COMPL_ANY=5.00 PRIMER_PAIR_562_COMPL_END=2.00 PRIMER_PAIR_562_PRODUCT_SIZE=97 PRIMER_PAIR_563_PENALTY=9.502348 PRIMER_LEFT_563_PENALTY=4.059923 PRIMER_RIGHT_563_PENALTY=5.442425 PRIMER_LEFT_563_SEQUENCE=ACACCAGGAGAGGATGGATGTT PRIMER_RIGHT_563_SEQUENCE=ATGCAAGTTGGTACTCTTCTCAC PRIMER_LEFT_563=607,22 PRIMER_RIGHT_563=697,23 PRIMER_LEFT_563_TM=62.060 PRIMER_RIGHT_563_TM=57.558 PRIMER_LEFT_563_GC_PERCENT=50.000 PRIMER_RIGHT_563_GC_PERCENT=43.478 PRIMER_LEFT_563_SELF_ANY=4.00 PRIMER_RIGHT_563_SELF_ANY=5.00 PRIMER_LEFT_563_SELF_END=0.00 PRIMER_RIGHT_563_SELF_END=0.00 PRIMER_LEFT_563_END_STABILITY=6.6000 PRIMER_RIGHT_563_END_STABILITY=6.4000 PRIMER_PAIR_563_COMPL_ANY=5.00 PRIMER_PAIR_563_COMPL_END=1.00 PRIMER_PAIR_563_PRODUCT_SIZE=91 PRIMER_PAIR_564_PENALTY=9.551609 PRIMER_LEFT_564_PENALTY=2.606855 PRIMER_RIGHT_564_PENALTY=6.944753 PRIMER_LEFT_564_SEQUENCE=CCAGAATGGGAAGTGGGA PRIMER_RIGHT_564_SEQUENCE=TTCAGGGTAGACTTCTTGGCAATAC PRIMER_LEFT_564=340,18 PRIMER_RIGHT_564=436,25 PRIMER_LEFT_564_TM=59.393 PRIMER_RIGHT_564_TM=61.945 PRIMER_LEFT_564_GC_PERCENT=55.556 PRIMER_RIGHT_564_GC_PERCENT=44.000 PRIMER_LEFT_564_SELF_ANY=3.00 PRIMER_RIGHT_564_SELF_ANY=5.00 PRIMER_LEFT_564_SELF_END=0.00 PRIMER_RIGHT_564_SELF_END=3.00 PRIMER_LEFT_564_END_STABILITY=9.7000 PRIMER_RIGHT_564_END_STABILITY=5.6000 PRIMER_PAIR_564_COMPL_ANY=5.00 PRIMER_PAIR_564_COMPL_END=1.00 PRIMER_PAIR_564_PRODUCT_SIZE=97 PRIMER_PAIR_565_PENALTY=9.639648 PRIMER_LEFT_565_PENALTY=4.184062 PRIMER_RIGHT_565_PENALTY=5.455586 PRIMER_LEFT_565_SEQUENCE=GTTTGCGAAACTCATCTTCACTG PRIMER_RIGHT_565_SEQUENCE=AGTCATGCAAGTTGGTACTCTTCT PRIMER_LEFT_565=626,23 PRIMER_RIGHT_565=701,24 PRIMER_LEFT_565_TM=61.184 PRIMER_RIGHT_565_TM=58.544 PRIMER_LEFT_565_GC_PERCENT=43.478 PRIMER_RIGHT_565_GC_PERCENT=41.667 PRIMER_LEFT_565_SELF_ANY=6.00 PRIMER_RIGHT_565_SELF_ANY=5.00 PRIMER_LEFT_565_SELF_END=3.00 PRIMER_RIGHT_565_SELF_END=2.00 PRIMER_LEFT_565_END_STABILITY=6.7000 PRIMER_RIGHT_565_END_STABILITY=6.7000 PRIMER_PAIR_565_COMPL_ANY=4.00 PRIMER_PAIR_565_COMPL_END=1.00 PRIMER_PAIR_565_PRODUCT_SIZE=76 PRIMER_PAIR_566_PENALTY=9.672380 PRIMER_LEFT_566_PENALTY=4.216794 PRIMER_RIGHT_566_PENALTY=5.455586 PRIMER_LEFT_566_SEQUENCE=TCTTACACCAGGAGAGGATGGAT PRIMER_RIGHT_566_SEQUENCE=ATGCAAGTTGGTACTCTTCTCACT PRIMER_LEFT_566=603,23 PRIMER_RIGHT_566=697,24 PRIMER_LEFT_566_TM=61.217 PRIMER_RIGHT_566_TM=58.544 PRIMER_LEFT_566_GC_PERCENT=47.826 PRIMER_RIGHT_566_GC_PERCENT=41.667 PRIMER_LEFT_566_SELF_ANY=3.00 PRIMER_RIGHT_566_SELF_ANY=5.00 PRIMER_LEFT_566_SELF_END=2.00 PRIMER_RIGHT_566_SELF_END=1.00 PRIMER_LEFT_566_END_STABILITY=8.1000 PRIMER_RIGHT_566_END_STABILITY=6.4000 PRIMER_PAIR_566_COMPL_ANY=5.00 PRIMER_PAIR_566_COMPL_END=3.00 PRIMER_PAIR_566_PRODUCT_SIZE=95 PRIMER_PAIR_567_PENALTY=9.731362 PRIMER_LEFT_567_PENALTY=5.016724 PRIMER_RIGHT_567_PENALTY=4.714638 PRIMER_LEFT_567_SEQUENCE=CTACCGCTGCTTAGTTGGTGAGT PRIMER_RIGHT_567_SEQUENCE=CATCCTCTCCTGGTGTAAGAAT PRIMER_LEFT_567=541,23 PRIMER_RIGHT_567=622,22 PRIMER_LEFT_567_TM=62.017 PRIMER_RIGHT_567_TM=57.285 PRIMER_LEFT_567_GC_PERCENT=52.174 PRIMER_RIGHT_567_GC_PERCENT=45.455 PRIMER_LEFT_567_SELF_ANY=3.00 PRIMER_RIGHT_567_SELF_ANY=3.00 PRIMER_LEFT_567_SELF_END=1.00 PRIMER_RIGHT_567_SELF_END=2.00 PRIMER_LEFT_567_END_STABILITY=6.4000 PRIMER_RIGHT_567_END_STABILITY=6.6000 PRIMER_PAIR_567_COMPL_ANY=4.00 PRIMER_PAIR_567_COMPL_END=1.00 PRIMER_PAIR_567_PRODUCT_SIZE=82 PRIMER_PAIR_568_PENALTY=9.747180 PRIMER_LEFT_568_PENALTY=5.455235 PRIMER_RIGHT_568_PENALTY=4.291945 PRIMER_LEFT_568_SEQUENCE=ATCCCCACTTTGTGATTCCCTAC PRIMER_RIGHT_568_SEQUENCE=TAAGAATTTGCACTTGTCAGGAAC PRIMER_LEFT_568=522,23 PRIMER_RIGHT_568=607,24 PRIMER_LEFT_568_TM=62.455 PRIMER_RIGHT_568_TM=59.708 PRIMER_LEFT_568_GC_PERCENT=47.826 PRIMER_RIGHT_568_GC_PERCENT=37.500 PRIMER_LEFT_568_SELF_ANY=4.00 PRIMER_RIGHT_568_SELF_ANY=4.00 PRIMER_LEFT_568_SELF_END=1.00 PRIMER_RIGHT_568_SELF_END=1.00 PRIMER_LEFT_568_END_STABILITY=6.9000 PRIMER_RIGHT_568_END_STABILITY=7.9000 PRIMER_PAIR_568_COMPL_ANY=4.00 PRIMER_PAIR_568_COMPL_END=3.00 PRIMER_PAIR_568_PRODUCT_SIZE=86 PRIMER_PAIR_569_PENALTY=9.749247 PRIMER_LEFT_569_PENALTY=4.847028 PRIMER_RIGHT_569_PENALTY=4.902219 PRIMER_LEFT_569_SEQUENCE=TTGCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_569_SEQUENCE=CACCAGTTCTGGATGGTCACTG PRIMER_LEFT_569=415,23 PRIMER_RIGHT_569=492,22 PRIMER_LEFT_569_TM=61.847 PRIMER_RIGHT_569_TM=62.902 PRIMER_LEFT_569_GC_PERCENT=47.826 PRIMER_RIGHT_569_GC_PERCENT=54.545 PRIMER_LEFT_569_SELF_ANY=5.00 PRIMER_RIGHT_569_SELF_ANY=6.00 PRIMER_LEFT_569_SELF_END=0.00 PRIMER_RIGHT_569_SELF_END=3.00 PRIMER_LEFT_569_END_STABILITY=6.7000 PRIMER_RIGHT_569_END_STABILITY=6.7000 PRIMER_PAIR_569_COMPL_ANY=5.00 PRIMER_PAIR_569_COMPL_END=3.00 PRIMER_PAIR_569_PRODUCT_SIZE=78 PRIMER_PAIR_570_PENALTY=9.880636 PRIMER_LEFT_570_PENALTY=3.678365 PRIMER_RIGHT_570_PENALTY=6.202271 PRIMER_LEFT_570_SEQUENCE=CCTGACAAGTGCAAATTCTTACA PRIMER_RIGHT_570_SEQUENCE=TACTCTTCTCACTGCATGTCTCTTT PRIMER_LEFT_570=587,23 PRIMER_RIGHT_570=686,25 PRIMER_LEFT_570_TM=59.322 PRIMER_RIGHT_570_TM=58.798 PRIMER_LEFT_570_GC_PERCENT=39.130 PRIMER_RIGHT_570_GC_PERCENT=40.000 PRIMER_LEFT_570_SELF_ANY=4.00 PRIMER_RIGHT_570_SELF_ANY=4.00 PRIMER_LEFT_570_SELF_END=3.00 PRIMER_RIGHT_570_SELF_END=0.00 PRIMER_LEFT_570_END_STABILITY=6.0000 PRIMER_RIGHT_570_END_STABILITY=7.0000 PRIMER_PAIR_570_COMPL_ANY=5.00 PRIMER_PAIR_570_COMPL_END=1.00 PRIMER_PAIR_570_PRODUCT_SIZE=100 PRIMER_PAIR_571_PENALTY=9.921431 PRIMER_LEFT_571_PENALTY=4.099075 PRIMER_RIGHT_571_PENALTY=5.822356 PRIMER_LEFT_571_SEQUENCE=AAATTCTTACACCAGGAGAGGATG PRIMER_RIGHT_571_SEQUENCE=AGTTGGTACTCTTCTCACTGCAT PRIMER_LEFT_571=599,24 PRIMER_RIGHT_571=692,23 PRIMER_LEFT_571_TM=59.901 PRIMER_RIGHT_571_TM=57.178 PRIMER_LEFT_571_GC_PERCENT=41.667 PRIMER_RIGHT_571_GC_PERCENT=43.478 PRIMER_LEFT_571_SELF_ANY=4.00 PRIMER_RIGHT_571_SELF_ANY=4.00 PRIMER_LEFT_571_SELF_END=0.00 PRIMER_RIGHT_571_SELF_END=2.00 PRIMER_LEFT_571_END_STABILITY=8.1000 PRIMER_RIGHT_571_END_STABILITY=8.4000 PRIMER_PAIR_571_COMPL_ANY=6.00 PRIMER_PAIR_571_COMPL_END=3.00 PRIMER_PAIR_571_PRODUCT_SIZE=94 PRIMER_PAIR_572_PENALTY=9.971012 PRIMER_LEFT_572_PENALTY=7.514914 PRIMER_RIGHT_572_PENALTY=2.456098 PRIMER_LEFT_572_SEQUENCE=AAGAGACATGCAGTGAGAAGAGTACC PRIMER_RIGHT_572_SEQUENCE=GCAACACACAAACTCTACCCCT PRIMER_LEFT_572=663,26 PRIMER_RIGHT_572=760,22 PRIMER_LEFT_572_TM=61.515 PRIMER_RIGHT_572_TM=60.456 PRIMER_LEFT_572_GC_PERCENT=46.154 PRIMER_RIGHT_572_GC_PERCENT=50.000 PRIMER_LEFT_572_SELF_ANY=4.00 PRIMER_RIGHT_572_SELF_ANY=2.00 PRIMER_LEFT_572_SELF_END=2.00 PRIMER_RIGHT_572_SELF_END=0.00 PRIMER_LEFT_572_END_STABILITY=6.6000 PRIMER_RIGHT_572_END_STABILITY=10.9000 PRIMER_PAIR_572_COMPL_ANY=5.00 PRIMER_PAIR_572_COMPL_END=2.00 PRIMER_PAIR_572_PRODUCT_SIZE=98 PRIMER_PAIR_573_PENALTY=10.145973 PRIMER_LEFT_573_PENALTY=3.809493 PRIMER_RIGHT_573_PENALTY=6.336480 PRIMER_LEFT_573_SEQUENCE=GACAAGTGCAAATTCTTACACCA PRIMER_RIGHT_573_SEQUENCE=ACTCTTCTCACTGCATGTCTCTTTG PRIMER_LEFT_573=590,23 PRIMER_RIGHT_573=685,25 PRIMER_LEFT_573_TM=59.191 PRIMER_RIGHT_573_TM=61.336 PRIMER_LEFT_573_GC_PERCENT=39.130 PRIMER_RIGHT_573_GC_PERCENT=44.000 PRIMER_LEFT_573_SELF_ANY=5.00 PRIMER_RIGHT_573_SELF_ANY=4.00 PRIMER_LEFT_573_SELF_END=1.00 PRIMER_RIGHT_573_SELF_END=0.00 PRIMER_LEFT_573_END_STABILITY=8.2000 PRIMER_RIGHT_573_END_STABILITY=7.3000 PRIMER_PAIR_573_COMPL_ANY=4.00 PRIMER_PAIR_573_COMPL_END=2.00 PRIMER_PAIR_573_PRODUCT_SIZE=96 PRIMER_PAIR_574_PENALTY=10.149444 PRIMER_LEFT_574_PENALTY=5.829268 PRIMER_RIGHT_574_PENALTY=4.320176 PRIMER_LEFT_574_SEQUENCE=CAATGTGGTAGAAGCCAACCAAC PRIMER_RIGHT_574_SEQUENCE=GTAGGGAATCACAAAGTGGGGA PRIMER_LEFT_574=448,23 PRIMER_RIGHT_574=544,22 PRIMER_LEFT_574_TM=62.829 PRIMER_RIGHT_574_TM=62.320 PRIMER_LEFT_574_GC_PERCENT=47.826 PRIMER_RIGHT_574_GC_PERCENT=50.000 PRIMER_LEFT_574_SELF_ANY=4.00 PRIMER_RIGHT_574_SELF_ANY=3.00 PRIMER_LEFT_574_SELF_END=2.00 PRIMER_RIGHT_574_SELF_END=2.00 PRIMER_LEFT_574_END_STABILITY=8.2000 PRIMER_RIGHT_574_END_STABILITY=10.9000 PRIMER_PAIR_574_COMPL_ANY=4.00 PRIMER_PAIR_574_COMPL_END=1.00 PRIMER_PAIR_574_PRODUCT_SIZE=97 PRIMER_PAIR_575_PENALTY=10.149702 PRIMER_LEFT_575_PENALTY=7.300506 PRIMER_RIGHT_575_PENALTY=2.849195 PRIMER_LEFT_575_SEQUENCE=CAAAGAGACATGCAGTGAGAAGAGTA PRIMER_RIGHT_575_SEQUENCE=CACACAAACTCTACCCCTCGG PRIMER_LEFT_575=661,26 PRIMER_RIGHT_575=756,21 PRIMER_LEFT_575_TM=61.301 PRIMER_RIGHT_575_TM=61.849 PRIMER_LEFT_575_GC_PERCENT=42.308 PRIMER_RIGHT_575_GC_PERCENT=57.143 PRIMER_LEFT_575_SELF_ANY=4.00 PRIMER_RIGHT_575_SELF_ANY=2.00 PRIMER_LEFT_575_SELF_END=2.00 PRIMER_RIGHT_575_SELF_END=2.00 PRIMER_LEFT_575_END_STABILITY=5.4000 PRIMER_RIGHT_575_END_STABILITY=9.9000 PRIMER_PAIR_575_COMPL_ANY=5.00 PRIMER_PAIR_575_COMPL_END=2.00 PRIMER_PAIR_575_PRODUCT_SIZE=96 PRIMER_PAIR_576_PENALTY=10.165261 PRIMER_LEFT_576_PENALTY=6.483283 PRIMER_RIGHT_576_PENALTY=3.681979 PRIMER_LEFT_576_SEQUENCE=CTGGAGGTACCCACTGATGGTAAT PRIMER_RIGHT_576_SEQUENCE=TGCATGTTCAGTCTGCCA PRIMER_LEFT_576=251,24 PRIMER_RIGHT_576=330,18 PRIMER_LEFT_576_TM=62.483 PRIMER_RIGHT_576_TM=58.318 PRIMER_LEFT_576_GC_PERCENT=50.000 PRIMER_RIGHT_576_GC_PERCENT=50.000 PRIMER_LEFT_576_SELF_ANY=6.00 PRIMER_RIGHT_576_SELF_ANY=5.00 PRIMER_LEFT_576_SELF_END=2.00 PRIMER_RIGHT_576_SELF_END=3.00 PRIMER_LEFT_576_END_STABILITY=5.6000 PRIMER_RIGHT_576_END_STABILITY=10.0000 PRIMER_PAIR_576_COMPL_ANY=5.00 PRIMER_PAIR_576_COMPL_END=1.00 PRIMER_PAIR_576_PRODUCT_SIZE=80 PRIMER_PAIR_577_PENALTY=10.172787 PRIMER_LEFT_577_PENALTY=4.099075 PRIMER_RIGHT_577_PENALTY=6.073713 PRIMER_LEFT_577_SEQUENCE=CAAATTCTTACACCAGGAGAGGAT PRIMER_RIGHT_577_SEQUENCE=CAAGTTGGTACTCTTCTCACTGCAT PRIMER_LEFT_577=598,24 PRIMER_RIGHT_577=694,25 PRIMER_LEFT_577_TM=59.901 PRIMER_RIGHT_577_TM=61.074 PRIMER_LEFT_577_GC_PERCENT=41.667 PRIMER_RIGHT_577_GC_PERCENT=44.000 PRIMER_LEFT_577_SELF_ANY=4.00 PRIMER_RIGHT_577_SELF_ANY=5.00 PRIMER_LEFT_577_SELF_END=2.00 PRIMER_RIGHT_577_SELF_END=2.00 PRIMER_LEFT_577_END_STABILITY=7.8000 PRIMER_RIGHT_577_END_STABILITY=8.4000 PRIMER_PAIR_577_COMPL_ANY=6.00 PRIMER_PAIR_577_COMPL_END=3.00 PRIMER_PAIR_577_PRODUCT_SIZE=97 PRIMER_PAIR_578_PENALTY=10.188964 PRIMER_LEFT_578_PENALTY=5.194065 PRIMER_RIGHT_578_PENALTY=4.994899 PRIMER_LEFT_578_SEQUENCE=TGCTTAGTTGGTGAGTTTGTAAGTG PRIMER_RIGHT_578_SEQUENCE=AACATCCATCCTCTCCTGGTGTA PRIMER_LEFT_578=548,25 PRIMER_RIGHT_578=628,23 PRIMER_LEFT_578_TM=59.806 PRIMER_RIGHT_578_TM=61.995 PRIMER_LEFT_578_GC_PERCENT=40.000 PRIMER_RIGHT_578_GC_PERCENT=47.826 PRIMER_LEFT_578_SELF_ANY=4.00 PRIMER_RIGHT_578_SELF_ANY=4.00 PRIMER_LEFT_578_SELF_END=0.00 PRIMER_RIGHT_578_SELF_END=2.00 PRIMER_LEFT_578_END_STABILITY=6.7000 PRIMER_RIGHT_578_END_STABILITY=5.4000 PRIMER_PAIR_578_COMPL_ANY=5.00 PRIMER_PAIR_578_COMPL_END=0.00 PRIMER_PAIR_578_PRODUCT_SIZE=81 PRIMER_PAIR_579_PENALTY=10.207732 PRIMER_LEFT_579_PENALTY=4.523430 PRIMER_RIGHT_579_PENALTY=5.684302 PRIMER_LEFT_579_SEQUENCE=ACCAGGAGAGGATGGATGTTTG PRIMER_RIGHT_579_SEQUENCE=GCAAGTTGGTACTCTTCTCACTGC PRIMER_LEFT_579=609,22 PRIMER_RIGHT_579=695,24 PRIMER_LEFT_579_TM=62.523 PRIMER_RIGHT_579_TM=61.684 PRIMER_LEFT_579_GC_PERCENT=50.000 PRIMER_RIGHT_579_GC_PERCENT=50.000 PRIMER_LEFT_579_SELF_ANY=3.00 PRIMER_RIGHT_579_SELF_ANY=5.00 PRIMER_LEFT_579_SELF_END=0.00 PRIMER_RIGHT_579_SELF_END=2.00 PRIMER_LEFT_579_END_STABILITY=7.0000 PRIMER_RIGHT_579_END_STABILITY=7.9000 PRIMER_PAIR_579_COMPL_ANY=6.00 PRIMER_PAIR_579_COMPL_END=1.00 PRIMER_PAIR_579_PRODUCT_SIZE=87 PRIMER_PAIR_580_PENALTY=10.225384 PRIMER_LEFT_580_PENALTY=5.338743 PRIMER_RIGHT_580_PENALTY=4.886641 PRIMER_LEFT_580_SEQUENCE=TACCGCTGCTTAGTTGGTGAGTT PRIMER_RIGHT_580_SEQUENCE=CTCCTGGTGTAAGAATTTGCACTT PRIMER_LEFT_580=542,23 PRIMER_RIGHT_580=616,24 PRIMER_LEFT_580_TM=62.339 PRIMER_RIGHT_580_TM=60.887 PRIMER_LEFT_580_GC_PERCENT=47.826 PRIMER_RIGHT_580_GC_PERCENT=41.667 PRIMER_LEFT_580_SELF_ANY=3.00 PRIMER_RIGHT_580_SELF_ANY=5.00 PRIMER_LEFT_580_SELF_END=0.00 PRIMER_RIGHT_580_SELF_END=2.00 PRIMER_LEFT_580_END_STABILITY=6.4000 PRIMER_RIGHT_580_END_STABILITY=6.7000 PRIMER_PAIR_580_COMPL_ANY=4.00 PRIMER_PAIR_580_COMPL_END=1.00 PRIMER_PAIR_580_PRODUCT_SIZE=75 PRIMER_PAIR_581_PENALTY=10.229938 PRIMER_LEFT_581_PENALTY=5.937993 PRIMER_RIGHT_581_PENALTY=4.291945 PRIMER_LEFT_581_SEQUENCE=GTGATTCCCTACCGCTGCTTAGT PRIMER_RIGHT_581_SEQUENCE=GTAAGAATTTGCACTTGTCAGGAA PRIMER_LEFT_581=533,23 PRIMER_RIGHT_581=608,24 PRIMER_LEFT_581_TM=62.938 PRIMER_RIGHT_581_TM=59.708 PRIMER_LEFT_581_GC_PERCENT=52.174 PRIMER_RIGHT_581_GC_PERCENT=37.500 PRIMER_LEFT_581_SELF_ANY=3.00 PRIMER_RIGHT_581_SELF_ANY=4.00 PRIMER_LEFT_581_SELF_END=1.00 PRIMER_RIGHT_581_SELF_END=0.00 PRIMER_LEFT_581_END_STABILITY=5.7000 PRIMER_RIGHT_581_END_STABILITY=8.2000 PRIMER_PAIR_581_COMPL_ANY=4.00 PRIMER_PAIR_581_COMPL_END=3.00 PRIMER_PAIR_581_PRODUCT_SIZE=76 PRIMER_PAIR_582_PENALTY=10.242055 PRIMER_LEFT_582_PENALTY=4.123386 PRIMER_RIGHT_582_PENALTY=6.118669 PRIMER_LEFT_582_SEQUENCE=CGAAACTCATCTTCACTGGCAC PRIMER_RIGHT_582_SEQUENCE=CCGTAGTCATGCAAGTTGGTACTC PRIMER_LEFT_582=631,22 PRIMER_RIGHT_582=705,24 PRIMER_LEFT_582_TM=62.123 PRIMER_RIGHT_582_TM=62.119 PRIMER_LEFT_582_GC_PERCENT=50.000 PRIMER_RIGHT_582_GC_PERCENT=50.000 PRIMER_LEFT_582_SELF_ANY=3.00 PRIMER_RIGHT_582_SELF_ANY=5.00 PRIMER_LEFT_582_SELF_END=1.00 PRIMER_RIGHT_582_SELF_END=2.00 PRIMER_LEFT_582_END_STABILITY=9.4000 PRIMER_RIGHT_582_END_STABILITY=5.4000 PRIMER_PAIR_582_COMPL_ANY=5.00 PRIMER_PAIR_582_COMPL_END=3.00 PRIMER_PAIR_582_PRODUCT_SIZE=75 PRIMER_PAIR_583_PENALTY=10.243737 PRIMER_LEFT_583_PENALTY=3.809493 PRIMER_RIGHT_583_PENALTY=6.434244 PRIMER_LEFT_583_SEQUENCE=TGACAAGTGCAAATTCTTACACC PRIMER_RIGHT_583_SEQUENCE=GTACTCTTCTCACTGCATGTCTCTTT PRIMER_LEFT_583=589,23 PRIMER_RIGHT_583=687,26 PRIMER_LEFT_583_TM=59.191 PRIMER_RIGHT_583_TM=59.566 PRIMER_LEFT_583_GC_PERCENT=39.130 PRIMER_RIGHT_583_GC_PERCENT=42.308 PRIMER_LEFT_583_SELF_ANY=5.00 PRIMER_RIGHT_583_SELF_ANY=4.00 PRIMER_LEFT_583_SELF_END=3.00 PRIMER_RIGHT_583_SELF_END=0.00 PRIMER_LEFT_583_END_STABILITY=7.6000 PRIMER_RIGHT_583_END_STABILITY=7.0000 PRIMER_PAIR_583_COMPL_ANY=5.00 PRIMER_PAIR_583_COMPL_END=0.00 PRIMER_PAIR_583_PRODUCT_SIZE=99 PRIMER_PAIR_584_PENALTY=10.285684 PRIMER_LEFT_584_PENALTY=6.612910 PRIMER_RIGHT_584_PENALTY=3.672773 PRIMER_LEFT_584_SEQUENCE=GAGGTACCCACTGATGGTAATGCT PRIMER_RIGHT_584_SEQUENCE=TTCCCATTCTGGACATTCA PRIMER_LEFT_584=254,24 PRIMER_RIGHT_584=351,19 PRIMER_LEFT_584_TM=62.613 PRIMER_RIGHT_584_TM=57.327 PRIMER_LEFT_584_GC_PERCENT=50.000 PRIMER_RIGHT_584_GC_PERCENT=42.105 PRIMER_LEFT_584_SELF_ANY=6.00 PRIMER_RIGHT_584_SELF_ANY=3.00 PRIMER_LEFT_584_SELF_END=0.00 PRIMER_RIGHT_584_SELF_END=2.00 PRIMER_LEFT_584_END_STABILITY=8.1000 PRIMER_RIGHT_584_END_STABILITY=6.9000 PRIMER_PAIR_584_COMPL_ANY=5.00 PRIMER_PAIR_584_COMPL_END=2.00 PRIMER_PAIR_584_PRODUCT_SIZE=98 PRIMER_PAIR_585_PENALTY=10.329938 PRIMER_LEFT_585_PENALTY=2.606855 PRIMER_RIGHT_585_PENALTY=7.723082 PRIMER_LEFT_585_SEQUENCE=TCCAGAATGGGAAGTGGG PRIMER_RIGHT_585_SEQUENCE=AGTTCAGGGTAGACTTCTTGGCAAT PRIMER_LEFT_585=339,18 PRIMER_RIGHT_585=438,25 PRIMER_LEFT_585_TM=59.393 PRIMER_RIGHT_585_TM=62.723 PRIMER_LEFT_585_GC_PERCENT=55.556 PRIMER_RIGHT_585_GC_PERCENT=44.000 PRIMER_LEFT_585_SELF_ANY=3.00 PRIMER_RIGHT_585_SELF_ANY=5.00 PRIMER_LEFT_585_SELF_END=0.00 PRIMER_RIGHT_585_SELF_END=3.00 PRIMER_LEFT_585_END_STABILITY=9.4000 PRIMER_RIGHT_585_END_STABILITY=8.4000 PRIMER_PAIR_585_COMPL_ANY=5.00 PRIMER_PAIR_585_COMPL_END=1.00 PRIMER_PAIR_585_PRODUCT_SIZE=100 PRIMER_PAIR_586_PENALTY=10.362638 PRIMER_LEFT_586_PENALTY=3.908647 PRIMER_RIGHT_586_PENALTY=6.453992 PRIMER_LEFT_586_SEQUENCE=CAAGTGCAAATTCTTACACCAGG PRIMER_RIGHT_586_SEQUENCE=GTTGGTACTCTTCTCACTGCATGTC PRIMER_LEFT_586=592,23 PRIMER_RIGHT_586=691,25 PRIMER_LEFT_586_TM=60.909 PRIMER_RIGHT_586_TM=61.454 PRIMER_LEFT_586_GC_PERCENT=43.478 PRIMER_RIGHT_586_GC_PERCENT=48.000 PRIMER_LEFT_586_SELF_ANY=5.00 PRIMER_RIGHT_586_SELF_ANY=4.00 PRIMER_LEFT_586_SELF_END=3.00 PRIMER_RIGHT_586_SELF_END=2.00 PRIMER_LEFT_586_END_STABILITY=9.7000 PRIMER_RIGHT_586_END_STABILITY=6.3000 PRIMER_PAIR_586_COMPL_ANY=4.00 PRIMER_PAIR_586_COMPL_END=1.00 PRIMER_PAIR_586_PRODUCT_SIZE=100 PRIMER_PAIR_587_PENALTY=10.418744 PRIMER_LEFT_587_PENALTY=4.184062 PRIMER_RIGHT_587_PENALTY=6.234682 PRIMER_LEFT_587_SEQUENCE=TGTTTGCGAAACTCATCTTCACT PRIMER_RIGHT_587_SEQUENCE=TAGTCATGCAAGTTGGTACTCTTCTC PRIMER_LEFT_587=625,23 PRIMER_RIGHT_587=702,26 PRIMER_LEFT_587_TM=61.184 PRIMER_RIGHT_587_TM=60.235 PRIMER_LEFT_587_GC_PERCENT=39.130 PRIMER_RIGHT_587_GC_PERCENT=42.308 PRIMER_LEFT_587_SELF_ANY=6.00 PRIMER_RIGHT_587_SELF_ANY=5.00 PRIMER_LEFT_587_SELF_END=1.00 PRIMER_RIGHT_587_SELF_END=0.00 PRIMER_LEFT_587_END_STABILITY=6.4000 PRIMER_RIGHT_587_END_STABILITY=6.7000 PRIMER_PAIR_587_COMPL_ANY=4.00 PRIMER_PAIR_587_COMPL_END=1.00 PRIMER_PAIR_587_PRODUCT_SIZE=78 PRIMER_PAIR_588_PENALTY=10.453756 PRIMER_LEFT_588_PENALTY=5.348422 PRIMER_RIGHT_588_PENALTY=5.105334 PRIMER_LEFT_588_SEQUENCE=TAGTTGGTGAGTTTGTAAGTGATGC PRIMER_RIGHT_588_SEQUENCE=CATCCATCCTCTCCTGGTGTAAG PRIMER_LEFT_588=552,25 PRIMER_RIGHT_588=626,23 PRIMER_LEFT_588_TM=59.652 PRIMER_RIGHT_588_TM=62.105 PRIMER_LEFT_588_GC_PERCENT=40.000 PRIMER_RIGHT_588_GC_PERCENT=52.174 PRIMER_LEFT_588_SELF_ANY=2.00 PRIMER_RIGHT_588_SELF_ANY=3.00 PRIMER_LEFT_588_SELF_END=2.00 PRIMER_RIGHT_588_SELF_END=1.00 PRIMER_LEFT_588_END_STABILITY=8.1000 PRIMER_RIGHT_588_END_STABILITY=5.7000 PRIMER_PAIR_588_COMPL_ANY=4.00 PRIMER_PAIR_588_COMPL_END=1.00 PRIMER_PAIR_588_PRODUCT_SIZE=75 PRIMER_PAIR_589_PENALTY=10.578836 PRIMER_LEFT_589_PENALTY=4.505124 PRIMER_RIGHT_589_PENALTY=6.073713 PRIMER_LEFT_589_SEQUENCE=GATGTTTGCGAAACTCATCTTCA PRIMER_RIGHT_589_SEQUENCE=ATGCAAGTTGGTACTCTTCTCACTG PRIMER_LEFT_589=623,23 PRIMER_RIGHT_589=697,25 PRIMER_LEFT_589_TM=61.505 PRIMER_RIGHT_589_TM=61.074 PRIMER_LEFT_589_GC_PERCENT=39.130 PRIMER_RIGHT_589_GC_PERCENT=44.000 PRIMER_LEFT_589_SELF_ANY=6.00 PRIMER_RIGHT_589_SELF_ANY=5.00 PRIMER_LEFT_589_SELF_END=1.00 PRIMER_RIGHT_589_SELF_END=3.00 PRIMER_LEFT_589_END_STABILITY=7.0000 PRIMER_RIGHT_589_END_STABILITY=6.7000 PRIMER_PAIR_589_COMPL_ANY=4.00 PRIMER_PAIR_589_COMPL_END=2.00 PRIMER_PAIR_589_PRODUCT_SIZE=75 PRIMER_PAIR_590_PENALTY=10.597143 PRIMER_LEFT_590_PENALTY=4.523430 PRIMER_RIGHT_590_PENALTY=6.073713 PRIMER_LEFT_590_SEQUENCE=CACCAGGAGAGGATGGATGTTT PRIMER_RIGHT_590_SEQUENCE=CATGCAAGTTGGTACTCTTCTCACT PRIMER_LEFT_590=608,22 PRIMER_RIGHT_590=698,25 PRIMER_LEFT_590_TM=62.523 PRIMER_RIGHT_590_TM=61.074 PRIMER_LEFT_590_GC_PERCENT=50.000 PRIMER_RIGHT_590_GC_PERCENT=44.000 PRIMER_LEFT_590_SELF_ANY=3.00 PRIMER_RIGHT_590_SELF_ANY=5.00 PRIMER_LEFT_590_SELF_END=0.00 PRIMER_RIGHT_590_SELF_END=1.00 PRIMER_LEFT_590_END_STABILITY=7.0000 PRIMER_RIGHT_590_END_STABILITY=6.4000 PRIMER_PAIR_590_COMPL_ANY=5.00 PRIMER_PAIR_590_COMPL_END=0.00 PRIMER_PAIR_590_PRODUCT_SIZE=91 PRIMER_PAIR_591_PENALTY=10.630119 PRIMER_LEFT_591_PENALTY=5.817794 PRIMER_RIGHT_591_PENALTY=4.812325 PRIMER_LEFT_591_SEQUENCE=ATCACCAATGTGGTAGAAGCCAA PRIMER_RIGHT_591_SEQUENCE=GATGGGTCTTGCACTGCT PRIMER_LEFT_591=443,23 PRIMER_RIGHT_591=524,18 PRIMER_LEFT_591_TM=62.818 PRIMER_RIGHT_591_TM=57.188 PRIMER_LEFT_591_GC_PERCENT=43.478 PRIMER_RIGHT_591_GC_PERCENT=55.556 PRIMER_LEFT_591_SELF_ANY=6.00 PRIMER_RIGHT_591_SELF_ANY=5.00 PRIMER_LEFT_591_SELF_END=0.00 PRIMER_RIGHT_591_SELF_END=3.00 PRIMER_LEFT_591_END_STABILITY=10.0000 PRIMER_RIGHT_591_END_STABILITY=8.2000 PRIMER_PAIR_591_COMPL_ANY=4.00 PRIMER_PAIR_591_COMPL_END=3.00 PRIMER_PAIR_591_PRODUCT_SIZE=82 PRIMER_PAIR_592_PENALTY=10.639515 PRIMER_LEFT_592_PENALTY=4.095371 PRIMER_RIGHT_592_PENALTY=6.544143 PRIMER_LEFT_592_SEQUENCE=GACAAGTGCAAATTCTTACACCAG PRIMER_RIGHT_592_SEQUENCE=TACTCTTCTCACTGCATGTCTCTT PRIMER_LEFT_592=590,24 PRIMER_RIGHT_592=686,24 PRIMER_LEFT_592_TM=60.095 PRIMER_RIGHT_592_TM=57.456 PRIMER_LEFT_592_GC_PERCENT=41.667 PRIMER_RIGHT_592_GC_PERCENT=41.667 PRIMER_LEFT_592_SELF_ANY=5.00 PRIMER_RIGHT_592_SELF_ANY=4.00 PRIMER_LEFT_592_SELF_END=3.00 PRIMER_RIGHT_592_SELF_END=0.00 PRIMER_LEFT_592_END_STABILITY=7.9000 PRIMER_RIGHT_592_END_STABILITY=6.7000 PRIMER_PAIR_592_COMPL_ANY=4.00 PRIMER_PAIR_592_COMPL_END=1.00 PRIMER_PAIR_592_PRODUCT_SIZE=97 PRIMER_PAIR_593_PENALTY=10.696596 PRIMER_LEFT_593_PENALTY=4.131746 PRIMER_RIGHT_593_PENALTY=6.564850 PRIMER_LEFT_593_SEQUENCE=CAAATTCTTACACCAGGAGAGG PRIMER_RIGHT_593_SEQUENCE=TTGGTACTCTTCTCACTGCATGTCT PRIMER_LEFT_593=598,22 PRIMER_RIGHT_593=690,25 PRIMER_LEFT_593_TM=57.868 PRIMER_RIGHT_593_TM=61.565 PRIMER_LEFT_593_GC_PERCENT=45.455 PRIMER_RIGHT_593_GC_PERCENT=44.000 PRIMER_LEFT_593_SELF_ANY=4.00 PRIMER_RIGHT_593_SELF_ANY=4.00 PRIMER_LEFT_593_SELF_END=1.00 PRIMER_RIGHT_593_SELF_END=0.00 PRIMER_LEFT_593_END_STABILITY=7.9000 PRIMER_RIGHT_593_END_STABILITY=6.4000 PRIMER_PAIR_593_COMPL_ANY=5.00 PRIMER_PAIR_593_COMPL_END=3.00 PRIMER_PAIR_593_PRODUCT_SIZE=93 PRIMER_PAIR_594_PENALTY=10.783912 PRIMER_LEFT_594_PENALTY=4.095371 PRIMER_RIGHT_594_PENALTY=6.688541 PRIMER_LEFT_594_SEQUENCE=CTGACAAGTGCAAATTCTTACACC PRIMER_RIGHT_594_SEQUENCE=GTACTCTTCTCACTGCATGTCTCTT PRIMER_LEFT_594=588,24 PRIMER_RIGHT_594=687,25 PRIMER_LEFT_594_TM=60.095 PRIMER_RIGHT_594_TM=58.311 PRIMER_LEFT_594_GC_PERCENT=41.667 PRIMER_RIGHT_594_GC_PERCENT=44.000 PRIMER_LEFT_594_SELF_ANY=5.00 PRIMER_RIGHT_594_SELF_ANY=4.00 PRIMER_LEFT_594_SELF_END=3.00 PRIMER_RIGHT_594_SELF_END=0.00 PRIMER_LEFT_594_END_STABILITY=7.6000 PRIMER_RIGHT_594_END_STABILITY=6.7000 PRIMER_PAIR_594_COMPL_ANY=5.00 PRIMER_PAIR_594_COMPL_END=0.00 PRIMER_PAIR_594_PRODUCT_SIZE=100 PRIMER_PAIR_595_PENALTY=10.917192 PRIMER_LEFT_595_PENALTY=5.704162 PRIMER_RIGHT_595_PENALTY=5.213030 PRIMER_LEFT_595_SEQUENCE=GCTGCTTAGTTGGTGAGTTTGTAAG PRIMER_RIGHT_595_SEQUENCE=TCCATCCTCTCCTGGTGTAAGAA PRIMER_LEFT_595=546,25 PRIMER_RIGHT_595=624,23 PRIMER_LEFT_595_TM=60.704 PRIMER_RIGHT_595_TM=62.213 PRIMER_LEFT_595_GC_PERCENT=44.000 PRIMER_RIGHT_595_GC_PERCENT=47.826 PRIMER_LEFT_595_SELF_ANY=4.00 PRIMER_RIGHT_595_SELF_ANY=3.00 PRIMER_LEFT_595_SELF_END=0.00 PRIMER_RIGHT_595_SELF_END=1.00 PRIMER_LEFT_595_END_STABILITY=5.7000 PRIMER_RIGHT_595_END_STABILITY=7.0000 PRIMER_PAIR_595_COMPL_ANY=4.00 PRIMER_PAIR_595_COMPL_END=1.00 PRIMER_PAIR_595_PRODUCT_SIZE=79 PRIMER_PAIR_596_PENALTY=10.932548 PRIMER_LEFT_596_PENALTY=4.587183 PRIMER_RIGHT_596_PENALTY=6.345365 PRIMER_LEFT_596_SEQUENCE=TTGCGAAACTCATCTTCACTGG PRIMER_RIGHT_596_SEQUENCE=TAGTCATGCAAGTTGGTACTCTTC PRIMER_LEFT_596=628,22 PRIMER_RIGHT_596=702,24 PRIMER_LEFT_596_TM=62.587 PRIMER_RIGHT_596_TM=57.655 PRIMER_LEFT_596_GC_PERCENT=45.455 PRIMER_RIGHT_596_GC_PERCENT=41.667 PRIMER_LEFT_596_SELF_ANY=4.00 PRIMER_RIGHT_596_SELF_ANY=5.00 PRIMER_LEFT_596_SELF_END=1.00 PRIMER_RIGHT_596_SELF_END=0.00 PRIMER_LEFT_596_END_STABILITY=7.9000 PRIMER_RIGHT_596_END_STABILITY=6.7000 PRIMER_PAIR_596_COMPL_ANY=4.00 PRIMER_PAIR_596_COMPL_END=1.00 PRIMER_PAIR_596_PRODUCT_SIZE=75 PRIMER_PAIR_597_PENALTY=11.146085 PRIMER_LEFT_597_PENALTY=2.612614 PRIMER_RIGHT_597_PENALTY=8.533470 PRIMER_LEFT_597_SEQUENCE=CCATCAGGGACCAAAACCTG PRIMER_RIGHT_597_SEQUENCE=GATCTGCAGTTCAGGGTAGACTTCTT PRIMER_LEFT_597=365,20 PRIMER_RIGHT_597=445,26 PRIMER_LEFT_597_TM=62.613 PRIMER_RIGHT_597_TM=62.533 PRIMER_LEFT_597_GC_PERCENT=55.000 PRIMER_RIGHT_597_GC_PERCENT=46.154 PRIMER_LEFT_597_SELF_ANY=4.00 PRIMER_RIGHT_597_SELF_ANY=8.00 PRIMER_LEFT_597_SELF_END=1.00 PRIMER_RIGHT_597_SELF_END=2.00 PRIMER_LEFT_597_END_STABILITY=7.9000 PRIMER_RIGHT_597_END_STABILITY=7.0000 PRIMER_PAIR_597_COMPL_ANY=4.00 PRIMER_PAIR_597_COMPL_END=1.00 PRIMER_PAIR_597_PRODUCT_SIZE=81 PRIMER_PAIR_598_PENALTY=11.200197 PRIMER_LEFT_598_PENALTY=2.644351 PRIMER_RIGHT_598_PENALTY=8.555845 PRIMER_LEFT_598_SEQUENCE=ATTCAGATCCATCAGGGACCA PRIMER_RIGHT_598_SEQUENCE=GTTCAGGGTAGACTTCTTGGCAATAC PRIMER_LEFT_598=357,21 PRIMER_RIGHT_598=437,26 PRIMER_LEFT_598_TM=61.644 PRIMER_RIGHT_598_TM=62.556 PRIMER_LEFT_598_GC_PERCENT=47.619 PRIMER_RIGHT_598_GC_PERCENT=46.154 PRIMER_LEFT_598_SELF_ANY=5.00 PRIMER_RIGHT_598_SELF_ANY=5.00 PRIMER_LEFT_598_SELF_END=3.00 PRIMER_RIGHT_598_SELF_END=3.00 PRIMER_LEFT_598_END_STABILITY=7.9000 PRIMER_RIGHT_598_END_STABILITY=5.6000 PRIMER_PAIR_598_COMPL_ANY=3.00 PRIMER_PAIR_598_COMPL_END=0.00 PRIMER_PAIR_598_PRODUCT_SIZE=81 PRIMER_PAIR_599_PENALTY=11.235681 PRIMER_LEFT_599_PENALTY=4.340634 PRIMER_RIGHT_599_PENALTY=6.895047 PRIMER_LEFT_599_SEQUENCE=AATTCTTACACCAGGAGAGGATG PRIMER_RIGHT_599_SEQUENCE=TCATGCAAGTTGGTACTCTTCTCAC PRIMER_LEFT_599=600,23 PRIMER_RIGHT_599=699,25 PRIMER_LEFT_599_TM=58.659 PRIMER_RIGHT_599_TM=61.895 PRIMER_LEFT_599_GC_PERCENT=43.478 PRIMER_RIGHT_599_GC_PERCENT=44.000 PRIMER_LEFT_599_SELF_ANY=4.00 PRIMER_RIGHT_599_SELF_ANY=5.00 PRIMER_LEFT_599_SELF_END=0.00 PRIMER_RIGHT_599_SELF_END=0.00 PRIMER_LEFT_599_END_STABILITY=8.1000 PRIMER_RIGHT_599_END_STABILITY=6.4000 PRIMER_PAIR_599_COMPL_ANY=5.00 PRIMER_PAIR_599_COMPL_END=3.00 PRIMER_PAIR_599_PRODUCT_SIZE=100 PRIMER_PAIR_600_PENALTY=11.291370 PRIMER_LEFT_600_PENALTY=2.644351 PRIMER_RIGHT_600_PENALTY=8.647019 PRIMER_LEFT_600_SEQUENCE=GTGGGATTCAGATCCATCAGG PRIMER_RIGHT_600_SEQUENCE=TTCAGGGTAGACTTCTTGGCAATACT PRIMER_LEFT_600=352,21 PRIMER_RIGHT_600=436,26 PRIMER_LEFT_600_TM=61.644 PRIMER_RIGHT_600_TM=62.647 PRIMER_LEFT_600_GC_PERCENT=52.381 PRIMER_RIGHT_600_GC_PERCENT=42.308 PRIMER_LEFT_600_SELF_ANY=5.00 PRIMER_RIGHT_600_SELF_ANY=5.00 PRIMER_LEFT_600_SELF_END=2.00 PRIMER_RIGHT_600_SELF_END=2.00 PRIMER_LEFT_600_END_STABILITY=8.2000 PRIMER_RIGHT_600_END_STABILITY=5.3000 PRIMER_PAIR_600_COMPL_ANY=3.00 PRIMER_PAIR_600_COMPL_END=1.00 PRIMER_PAIR_600_PRODUCT_SIZE=85 PRIMER_PAIR_601_PENALTY=11.330204 PRIMER_LEFT_601_PENALTY=4.933928 PRIMER_RIGHT_601_PENALTY=6.396277 PRIMER_LEFT_601_SEQUENCE=AGAGGATGGATGTTTGCG PRIMER_RIGHT_601_SEQUENCE=TAGTCATGCAAGTTGGTACTCTTCT PRIMER_LEFT_601=615,18 PRIMER_RIGHT_601=702,25 PRIMER_LEFT_601_TM=57.066 PRIMER_RIGHT_601_TM=58.604 PRIMER_LEFT_601_GC_PERCENT=50.000 PRIMER_RIGHT_601_GC_PERCENT=40.000 PRIMER_LEFT_601_SELF_ANY=2.00 PRIMER_RIGHT_601_SELF_ANY=5.00 PRIMER_LEFT_601_SELF_END=2.00 PRIMER_RIGHT_601_SELF_END=2.00 PRIMER_LEFT_601_END_STABILITY=10.5000 PRIMER_RIGHT_601_END_STABILITY=6.7000 PRIMER_PAIR_601_COMPL_ANY=4.00 PRIMER_PAIR_601_COMPL_END=0.00 PRIMER_PAIR_601_PRODUCT_SIZE=88 PRIMER_PAIR_602_PENALTY=11.404647 PRIMER_LEFT_602_PENALTY=4.326727 PRIMER_RIGHT_602_PENALTY=7.077920 PRIMER_LEFT_602_SEQUENCE=GCAAATTCTTACACCAGGAGAGG PRIMER_RIGHT_602_SEQUENCE=TGGTACTCTTCTCACTGCATGTCTC PRIMER_LEFT_602=597,23 PRIMER_RIGHT_602=689,25 PRIMER_LEFT_602_TM=61.327 PRIMER_RIGHT_602_TM=62.078 PRIMER_LEFT_602_GC_PERCENT=47.826 PRIMER_RIGHT_602_GC_PERCENT=48.000 PRIMER_LEFT_602_SELF_ANY=4.00 PRIMER_RIGHT_602_SELF_ANY=4.00 PRIMER_LEFT_602_SELF_END=1.00 PRIMER_RIGHT_602_SELF_END=0.00 PRIMER_LEFT_602_END_STABILITY=7.9000 PRIMER_RIGHT_602_END_STABILITY=6.1000 PRIMER_PAIR_602_COMPL_ANY=5.00 PRIMER_PAIR_602_COMPL_END=2.00 PRIMER_PAIR_602_PRODUCT_SIZE=93 PRIMER_PAIR_603_PENALTY=11.492613 PRIMER_LEFT_603_PENALTY=5.867986 PRIMER_RIGHT_603_PENALTY=5.624627 PRIMER_LEFT_603_SEQUENCE=CGCTGCTTAGTTGGTGAGTTTGT PRIMER_RIGHT_603_SEQUENCE=ATCCTCTCCTGGTGTAAGAATTT PRIMER_LEFT_603=545,23 PRIMER_RIGHT_603=621,23 PRIMER_LEFT_603_TM=62.868 PRIMER_RIGHT_603_TM=57.375 PRIMER_LEFT_603_GC_PERCENT=47.826 PRIMER_RIGHT_603_GC_PERCENT=39.130 PRIMER_LEFT_603_SELF_ANY=3.00 PRIMER_RIGHT_603_SELF_ANY=4.00 PRIMER_LEFT_603_SELF_END=0.00 PRIMER_RIGHT_603_SELF_END=3.00 PRIMER_LEFT_603_END_STABILITY=7.0000 PRIMER_RIGHT_603_END_STABILITY=7.2000 PRIMER_PAIR_603_COMPL_ANY=4.00 PRIMER_PAIR_603_COMPL_END=1.00 PRIMER_PAIR_603_PRODUCT_SIZE=77 PRIMER_PAIR_604_PENALTY=11.519840 PRIMER_LEFT_604_PENALTY=4.994899 PRIMER_RIGHT_604_PENALTY=6.524942 PRIMER_LEFT_604_SEQUENCE=TACACCAGGAGAGGATGGATGTT PRIMER_RIGHT_604_SEQUENCE=GTAGTCATGCAAGTTGGTACTCTTC PRIMER_LEFT_604=606,23 PRIMER_RIGHT_604=703,25 PRIMER_LEFT_604_TM=61.995 PRIMER_RIGHT_604_TM=58.475 PRIMER_LEFT_604_GC_PERCENT=47.826 PRIMER_RIGHT_604_GC_PERCENT=44.000 PRIMER_LEFT_604_SELF_ANY=4.00 PRIMER_RIGHT_604_SELF_ANY=5.00 PRIMER_LEFT_604_SELF_END=0.00 PRIMER_RIGHT_604_SELF_END=0.00 PRIMER_LEFT_604_END_STABILITY=6.6000 PRIMER_RIGHT_604_END_STABILITY=6.7000 PRIMER_PAIR_604_COMPL_ANY=4.00 PRIMER_PAIR_604_COMPL_END=1.00 PRIMER_PAIR_604_PRODUCT_SIZE=98 PRIMER_PAIR_605_PENALTY=11.609685 PRIMER_LEFT_605_PENALTY=4.714638 PRIMER_RIGHT_605_PENALTY=6.895047 PRIMER_LEFT_605_SEQUENCE=ATTCTTACACCAGGAGAGGATG PRIMER_RIGHT_605_SEQUENCE=GTCATGCAAGTTGGTACTCTTCTCA PRIMER_LEFT_605=601,22 PRIMER_RIGHT_605=700,25 PRIMER_LEFT_605_TM=57.285 PRIMER_RIGHT_605_TM=61.895 PRIMER_LEFT_605_GC_PERCENT=45.455 PRIMER_RIGHT_605_GC_PERCENT=44.000 PRIMER_LEFT_605_SELF_ANY=3.00 PRIMER_RIGHT_605_SELF_ANY=5.00 PRIMER_LEFT_605_SELF_END=0.00 PRIMER_RIGHT_605_SELF_END=1.00 PRIMER_LEFT_605_END_STABILITY=8.1000 PRIMER_RIGHT_605_END_STABILITY=6.7000 PRIMER_PAIR_605_COMPL_ANY=5.00 PRIMER_PAIR_605_COMPL_END=3.00 PRIMER_PAIR_605_PRODUCT_SIZE=100 PRIMER_PAIR_606_PENALTY=11.636080 PRIMER_LEFT_606_PENALTY=4.994899 PRIMER_RIGHT_606_PENALTY=6.641181 PRIMER_LEFT_606_SEQUENCE=TTACACCAGGAGAGGATGGATGT PRIMER_RIGHT_606_SEQUENCE=GTAGTCATGCAAGTTGGTACTCTTCT PRIMER_LEFT_606=605,23 PRIMER_RIGHT_606=703,26 PRIMER_LEFT_606_TM=61.995 PRIMER_RIGHT_606_TM=59.359 PRIMER_LEFT_606_GC_PERCENT=47.826 PRIMER_RIGHT_606_GC_PERCENT=42.308 PRIMER_LEFT_606_SELF_ANY=4.00 PRIMER_RIGHT_606_SELF_ANY=5.00 PRIMER_LEFT_606_SELF_END=0.00 PRIMER_RIGHT_606_SELF_END=2.00 PRIMER_LEFT_606_END_STABILITY=6.3000 PRIMER_RIGHT_606_END_STABILITY=6.7000 PRIMER_PAIR_606_COMPL_ANY=5.00 PRIMER_PAIR_606_COMPL_END=2.00 PRIMER_PAIR_606_PRODUCT_SIZE=99 PRIMER_PAIR_607_PENALTY=11.787753 PRIMER_LEFT_607_PENALTY=4.487247 PRIMER_RIGHT_607_PENALTY=7.300506 PRIMER_LEFT_607_SEQUENCE=CAAGTGCAAATTCTTACACCAG PRIMER_RIGHT_607_SEQUENCE=TACTCTTCTCACTGCATGTCTCTTTG PRIMER_LEFT_607=592,22 PRIMER_RIGHT_607=686,26 PRIMER_LEFT_607_TM=57.513 PRIMER_RIGHT_607_TM=61.301 PRIMER_LEFT_607_GC_PERCENT=40.909 PRIMER_RIGHT_607_GC_PERCENT=42.308 PRIMER_LEFT_607_SELF_ANY=5.00 PRIMER_RIGHT_607_SELF_ANY=4.00 PRIMER_LEFT_607_SELF_END=3.00 PRIMER_RIGHT_607_SELF_END=0.00 PRIMER_LEFT_607_END_STABILITY=7.9000 PRIMER_RIGHT_607_END_STABILITY=7.3000 PRIMER_PAIR_607_COMPL_ANY=4.00 PRIMER_PAIR_607_COMPL_END=1.00 PRIMER_PAIR_607_PRODUCT_SIZE=95 PRIMER_PAIR_608_PENALTY=12.139743 PRIMER_LEFT_608_PENALTY=4.624828 PRIMER_RIGHT_608_PENALTY=7.514914 PRIMER_LEFT_608_SEQUENCE=ACAAGTGCAAATTCTTACACCAG PRIMER_RIGHT_608_SEQUENCE=GGTACTCTTCTCACTGCATGTCTCTT PRIMER_LEFT_608=591,23 PRIMER_RIGHT_608=688,26 PRIMER_LEFT_608_TM=58.375 PRIMER_RIGHT_608_TM=61.515 PRIMER_LEFT_608_GC_PERCENT=39.130 PRIMER_RIGHT_608_GC_PERCENT=46.154 PRIMER_LEFT_608_SELF_ANY=5.00 PRIMER_RIGHT_608_SELF_ANY=4.00 PRIMER_LEFT_608_SELF_END=3.00 PRIMER_RIGHT_608_SELF_END=0.00 PRIMER_LEFT_608_END_STABILITY=7.9000 PRIMER_RIGHT_608_END_STABILITY=6.7000 PRIMER_PAIR_608_COMPL_ANY=4.00 PRIMER_PAIR_608_COMPL_END=1.00 PRIMER_PAIR_608_PRODUCT_SIZE=98 PRIMER_PAIR_609_PENALTY=12.368775 PRIMER_LEFT_609_PENALTY=6.015654 PRIMER_RIGHT_609_PENALTY=6.353121 PRIMER_LEFT_609_SEQUENCE=GCTTAGTTGGTGAGTTTGTAAGTGA PRIMER_RIGHT_609_SEQUENCE=TCCATCCTCTCCTGGTGTAAGAAT PRIMER_LEFT_609=549,25 PRIMER_RIGHT_609=624,24 PRIMER_LEFT_609_TM=58.984 PRIMER_RIGHT_609_TM=62.353 PRIMER_LEFT_609_GC_PERCENT=40.000 PRIMER_RIGHT_609_GC_PERCENT=45.833 PRIMER_LEFT_609_SELF_ANY=4.00 PRIMER_RIGHT_609_SELF_ANY=3.00 PRIMER_LEFT_609_SELF_END=1.00 PRIMER_RIGHT_609_SELF_END=2.00 PRIMER_LEFT_609_END_STABILITY=6.4000 PRIMER_RIGHT_609_END_STABILITY=6.6000 PRIMER_PAIR_609_COMPL_ANY=4.00 PRIMER_PAIR_609_COMPL_END=1.00 PRIMER_PAIR_609_PRODUCT_SIZE=76 PRIMER_PAIR_610_PENALTY=12.415579 PRIMER_LEFT_610_PENALTY=9.559679 PRIMER_RIGHT_610_PENALTY=2.855901 PRIMER_LEFT_610_SEQUENCE=AAAGAGACATGCAGTGAGAAGAGTACC PRIMER_RIGHT_610_SEQUENCE=CACAAACTCTACCCCTCGG PRIMER_LEFT_610=662,27 PRIMER_RIGHT_610=754,19 PRIMER_LEFT_610_TM=62.560 PRIMER_RIGHT_610_TM=58.144 PRIMER_LEFT_610_GC_PERCENT=44.444 PRIMER_RIGHT_610_GC_PERCENT=57.895 PRIMER_LEFT_610_SELF_ANY=4.00 PRIMER_RIGHT_610_SELF_ANY=2.00 PRIMER_LEFT_610_SELF_END=2.00 PRIMER_RIGHT_610_SELF_END=2.00 PRIMER_LEFT_610_END_STABILITY=6.6000 PRIMER_RIGHT_610_END_STABILITY=9.9000 PRIMER_PAIR_610_COMPL_ANY=5.00 PRIMER_PAIR_610_COMPL_END=2.00 PRIMER_PAIR_610_PRODUCT_SIZE=93 PRIMER_PAIR_611_PENALTY=12.917820 PRIMER_LEFT_611_PENALTY=6.564699 PRIMER_RIGHT_611_PENALTY=6.353121 PRIMER_LEFT_611_SEQUENCE=CTGCTTAGTTGGTGAGTTTGTAAG PRIMER_RIGHT_611_SEQUENCE=ATCCATCCTCTCCTGGTGTAAGAA PRIMER_LEFT_611=547,24 PRIMER_RIGHT_611=625,24 PRIMER_LEFT_611_TM=57.435 PRIMER_RIGHT_611_TM=62.353 PRIMER_LEFT_611_GC_PERCENT=41.667 PRIMER_RIGHT_611_GC_PERCENT=45.833 PRIMER_LEFT_611_SELF_ANY=4.00 PRIMER_RIGHT_611_SELF_ANY=3.00 PRIMER_LEFT_611_SELF_END=0.00 PRIMER_RIGHT_611_SELF_END=1.00 PRIMER_LEFT_611_END_STABILITY=5.7000 PRIMER_RIGHT_611_END_STABILITY=7.0000 PRIMER_PAIR_611_COMPL_ANY=4.00 PRIMER_PAIR_611_COMPL_END=1.00 PRIMER_PAIR_611_PRODUCT_SIZE=79 PRIMER_PAIR_612_PENALTY=13.022098 PRIMER_LEFT_612_PENALTY=5.105334 PRIMER_RIGHT_612_PENALTY=7.916764 PRIMER_LEFT_612_SEQUENCE=CTTACACCAGGAGAGGATGGATG PRIMER_RIGHT_612_SEQUENCE=GTAGTCATGCAAGTTGGTACTCTTCTC PRIMER_LEFT_612=604,23 PRIMER_RIGHT_612=703,27 PRIMER_LEFT_612_TM=62.105 PRIMER_RIGHT_612_TM=60.917 PRIMER_LEFT_612_GC_PERCENT=52.174 PRIMER_RIGHT_612_GC_PERCENT=44.444 PRIMER_LEFT_612_SELF_ANY=3.00 PRIMER_RIGHT_612_SELF_ANY=5.00 PRIMER_LEFT_612_SELF_END=0.00 PRIMER_RIGHT_612_SELF_END=0.00 PRIMER_LEFT_612_END_STABILITY=8.1000 PRIMER_RIGHT_612_END_STABILITY=6.7000 PRIMER_PAIR_612_COMPL_ANY=5.00 PRIMER_PAIR_612_COMPL_END=2.00 PRIMER_PAIR_612_PRODUCT_SIZE=100 PRIMER_PAIR_613_PENALTY=13.033059 PRIMER_LEFT_613_PENALTY=5.172847 PRIMER_RIGHT_613_PENALTY=7.860212 PRIMER_LEFT_613_SEQUENCE=GGATGTTTGCGAAACTCATCTTC PRIMER_RIGHT_613_SEQUENCE=CCGTAGTCATGCAAGTTGGTACTCT PRIMER_LEFT_613=622,23 PRIMER_RIGHT_613=705,25 PRIMER_LEFT_613_TM=62.173 PRIMER_RIGHT_613_TM=62.860 PRIMER_LEFT_613_GC_PERCENT=43.478 PRIMER_RIGHT_613_GC_PERCENT=48.000 PRIMER_LEFT_613_SELF_ANY=6.00 PRIMER_RIGHT_613_SELF_ANY=5.00 PRIMER_LEFT_613_SELF_END=3.00 PRIMER_RIGHT_613_SELF_END=0.00 PRIMER_LEFT_613_END_STABILITY=6.7000 PRIMER_RIGHT_613_END_STABILITY=6.1000 PRIMER_PAIR_613_COMPL_ANY=4.00 PRIMER_PAIR_613_COMPL_END=1.00 PRIMER_PAIR_613_PRODUCT_SIZE=84 PRIMER_PAIR_614_PENALTY=13.125715 PRIMER_LEFT_614_PENALTY=4.886641 PRIMER_RIGHT_614_PENALTY=8.239075 PRIMER_LEFT_614_SEQUENCE=AAGTGCAAATTCTTACACCAGGAG PRIMER_RIGHT_614_SEQUENCE=GTTGGTACTCTTCTCACTGCATGTCT PRIMER_LEFT_614=593,24 PRIMER_RIGHT_614=691,26 PRIMER_LEFT_614_TM=60.887 PRIMER_RIGHT_614_TM=62.239 PRIMER_LEFT_614_GC_PERCENT=41.667 PRIMER_RIGHT_614_GC_PERCENT=46.154 PRIMER_LEFT_614_SELF_ANY=5.00 PRIMER_RIGHT_614_SELF_ANY=4.00 PRIMER_LEFT_614_SELF_END=3.00 PRIMER_RIGHT_614_SELF_END=0.00 PRIMER_LEFT_614_END_STABILITY=7.9000 PRIMER_RIGHT_614_END_STABILITY=6.4000 PRIMER_PAIR_614_COMPL_ANY=4.00 PRIMER_PAIR_614_COMPL_END=2.00 PRIMER_PAIR_614_PRODUCT_SIZE=99 PRIMER_PAIR_615_PENALTY=13.401367 PRIMER_LEFT_615_PENALTY=6.619809 PRIMER_RIGHT_615_PENALTY=6.781558 PRIMER_LEFT_615_SEQUENCE=CTGCTTAGTTGGTGAGTTTGTAAGTG PRIMER_RIGHT_615_SEQUENCE=ACATCCATCCTCTCCTGGTGTAAG PRIMER_LEFT_615=547,26 PRIMER_RIGHT_615=627,24 PRIMER_LEFT_615_TM=60.620 PRIMER_RIGHT_615_TM=62.782 PRIMER_LEFT_615_GC_PERCENT=42.308 PRIMER_RIGHT_615_GC_PERCENT=50.000 PRIMER_LEFT_615_SELF_ANY=4.00 PRIMER_RIGHT_615_SELF_ANY=4.00 PRIMER_LEFT_615_SELF_END=0.00 PRIMER_RIGHT_615_SELF_END=1.00 PRIMER_LEFT_615_END_STABILITY=6.7000 PRIMER_RIGHT_615_END_STABILITY=5.7000 PRIMER_PAIR_615_COMPL_ANY=5.00 PRIMER_PAIR_615_COMPL_END=2.00 PRIMER_PAIR_615_PRODUCT_SIZE=81 PRIMER_PAIR_616_PENALTY=13.484150 PRIMER_LEFT_616_PENALTY=4.636384 PRIMER_RIGHT_616_PENALTY=8.847766 PRIMER_LEFT_616_SEQUENCE=ACAAGTGCAAATTCTTACACCA PRIMER_RIGHT_616_SEQUENCE=TGGTACTCTTCTCACTGCATGTCTCT PRIMER_LEFT_616=591,22 PRIMER_RIGHT_616=689,26 PRIMER_LEFT_616_TM=57.364 PRIMER_RIGHT_616_TM=62.848 PRIMER_LEFT_616_GC_PERCENT=36.364 PRIMER_RIGHT_616_GC_PERCENT=46.154 PRIMER_LEFT_616_SELF_ANY=5.00 PRIMER_RIGHT_616_SELF_ANY=4.00 PRIMER_LEFT_616_SELF_END=1.00 PRIMER_RIGHT_616_SELF_END=0.00 PRIMER_LEFT_616_END_STABILITY=8.2000 PRIMER_RIGHT_616_END_STABILITY=6.4000 PRIMER_PAIR_616_COMPL_ANY=4.00 PRIMER_PAIR_616_COMPL_END=1.00 PRIMER_PAIR_616_PRODUCT_SIZE=99 PRIMER_PAIR_617_PENALTY=13.602746 PRIMER_LEFT_617_PENALTY=5.363671 PRIMER_RIGHT_617_PENALTY=8.239075 PRIMER_LEFT_617_SEQUENCE=GTGCAAATTCTTACACCAGGAGAG PRIMER_RIGHT_617_SEQUENCE=AGTTGGTACTCTTCTCACTGCATGTC PRIMER_LEFT_617=595,24 PRIMER_RIGHT_617=692,26 PRIMER_LEFT_617_TM=61.364 PRIMER_RIGHT_617_TM=62.239 PRIMER_LEFT_617_GC_PERCENT=45.833 PRIMER_RIGHT_617_GC_PERCENT=46.154 PRIMER_LEFT_617_SELF_ANY=5.00 PRIMER_RIGHT_617_SELF_ANY=4.00 PRIMER_LEFT_617_SELF_END=0.00 PRIMER_RIGHT_617_SELF_END=2.00 PRIMER_LEFT_617_END_STABILITY=6.4000 PRIMER_RIGHT_617_END_STABILITY=6.3000 PRIMER_PAIR_617_COMPL_ANY=5.00 PRIMER_PAIR_617_COMPL_END=3.00 PRIMER_PAIR_617_PRODUCT_SIZE=98 PRIMER_PAIR_618_PENALTY=13.691286 PRIMER_LEFT_618_PENALTY=6.780955 PRIMER_RIGHT_618_PENALTY=6.910331 PRIMER_LEFT_618_SEQUENCE=CGCTGCTTAGTTGGTGAGTTTGTA PRIMER_RIGHT_618_SEQUENCE=TCCTCTCCTGGTGTAAGAATTTGC PRIMER_LEFT_618=545,24 PRIMER_RIGHT_618=620,24 PRIMER_LEFT_618_TM=62.781 PRIMER_RIGHT_618_TM=62.910 PRIMER_LEFT_618_GC_PERCENT=45.833 PRIMER_RIGHT_618_GC_PERCENT=45.833 PRIMER_LEFT_618_SELF_ANY=3.00 PRIMER_RIGHT_618_SELF_ANY=4.00 PRIMER_LEFT_618_SELF_END=2.00 PRIMER_RIGHT_618_SELF_END=2.00 PRIMER_LEFT_618_END_STABILITY=6.0000 PRIMER_RIGHT_618_END_STABILITY=8.8000 PRIMER_PAIR_618_COMPL_ANY=4.00 PRIMER_PAIR_618_COMPL_END=1.00 PRIMER_PAIR_618_PRODUCT_SIZE=76 PRIMER_PAIR_619_PENALTY=13.766602 PRIMER_LEFT_619_PENALTY=5.213030 PRIMER_RIGHT_619_PENALTY=8.553572 PRIMER_LEFT_619_SEQUENCE=TTCTTACACCAGGAGAGGATGGA PRIMER_RIGHT_619_SEQUENCE=GTCATGCAAGTTGGTACTCTTCTCAC PRIMER_LEFT_619=602,23 PRIMER_RIGHT_619=700,26 PRIMER_LEFT_619_TM=62.213 PRIMER_RIGHT_619_TM=62.554 PRIMER_LEFT_619_GC_PERCENT=47.826 PRIMER_RIGHT_619_GC_PERCENT=46.154 PRIMER_LEFT_619_SELF_ANY=3.00 PRIMER_RIGHT_619_SELF_ANY=5.00 PRIMER_LEFT_619_SELF_END=0.00 PRIMER_RIGHT_619_SELF_END=0.00 PRIMER_LEFT_619_END_STABILITY=8.1000 PRIMER_RIGHT_619_END_STABILITY=6.4000 PRIMER_PAIR_619_COMPL_ANY=5.00 PRIMER_PAIR_619_COMPL_END=3.00 PRIMER_PAIR_619_PRODUCT_SIZE=99 PRIMER_PAIR_620_PENALTY=13.853960 PRIMER_LEFT_620_PENALTY=5.374686 PRIMER_RIGHT_620_PENALTY=8.479274 PRIMER_LEFT_620_SEQUENCE=ATGTTTGCGAAACTCATCTTCACT PRIMER_RIGHT_620_SEQUENCE=CGTAGTCATGCAAGTTGGTACTCTTC PRIMER_LEFT_620=624,24 PRIMER_RIGHT_620=704,26 PRIMER_LEFT_620_TM=61.375 PRIMER_RIGHT_620_TM=62.479 PRIMER_LEFT_620_GC_PERCENT=37.500 PRIMER_RIGHT_620_GC_PERCENT=46.154 PRIMER_LEFT_620_SELF_ANY=6.00 PRIMER_RIGHT_620_SELF_ANY=5.00 PRIMER_LEFT_620_SELF_END=1.00 PRIMER_RIGHT_620_SELF_END=0.00 PRIMER_LEFT_620_END_STABILITY=6.4000 PRIMER_RIGHT_620_END_STABILITY=6.7000 PRIMER_PAIR_620_COMPL_ANY=4.00 PRIMER_PAIR_620_COMPL_END=1.00 PRIMER_PAIR_620_PRODUCT_SIZE=81 PRIMER_PAIR_621_PENALTY=14.001421 PRIMER_LEFT_621_PENALTY=6.702492 PRIMER_RIGHT_621_PENALTY=7.298929 PRIMER_LEFT_621_SEQUENCE=TGCTTAGTTGGTGAGTTTGTAAGT PRIMER_RIGHT_621_SEQUENCE=CATCCTCTCCTGGTGTAAGAATTTG PRIMER_LEFT_621=548,24 PRIMER_RIGHT_621=622,25 PRIMER_LEFT_621_TM=57.298 PRIMER_RIGHT_621_TM=62.299 PRIMER_LEFT_621_GC_PERCENT=37.500 PRIMER_RIGHT_621_GC_PERCENT=44.000 PRIMER_LEFT_621_SELF_ANY=4.00 PRIMER_RIGHT_621_SELF_ANY=4.00 PRIMER_LEFT_621_SELF_END=1.00 PRIMER_RIGHT_621_SELF_END=1.00 PRIMER_LEFT_621_END_STABILITY=5.7000 PRIMER_RIGHT_621_END_STABILITY=7.2000 PRIMER_PAIR_621_COMPL_ANY=4.00 PRIMER_PAIR_621_COMPL_END=1.00 PRIMER_PAIR_621_PRODUCT_SIZE=75 PRIMER_PAIR_622_PENALTY=14.182676 PRIMER_LEFT_622_PENALTY=6.702492 PRIMER_RIGHT_622_PENALTY=7.480183 PRIMER_LEFT_622_SEQUENCE=GCTTAGTTGGTGAGTTTGTAAGTG PRIMER_RIGHT_622_SEQUENCE=ATCCATCCTCTCCTGGTGTAAGAAT PRIMER_LEFT_622=549,24 PRIMER_RIGHT_622=625,25 PRIMER_LEFT_622_TM=57.298 PRIMER_RIGHT_622_TM=62.480 PRIMER_LEFT_622_GC_PERCENT=41.667 PRIMER_RIGHT_622_GC_PERCENT=44.000 PRIMER_LEFT_622_SELF_ANY=4.00 PRIMER_RIGHT_622_SELF_ANY=3.00 PRIMER_LEFT_622_SELF_END=0.00 PRIMER_RIGHT_622_SELF_END=2.00 PRIMER_LEFT_622_END_STABILITY=6.7000 PRIMER_RIGHT_622_END_STABILITY=6.6000 PRIMER_PAIR_622_COMPL_ANY=4.00 PRIMER_PAIR_622_COMPL_END=0.00 PRIMER_PAIR_622_PRODUCT_SIZE=77 PRIMER_PAIR_623_PENALTY=14.506108 PRIMER_LEFT_623_PENALTY=5.854573 PRIMER_RIGHT_623_PENALTY=8.651535 PRIMER_LEFT_623_SEQUENCE=AATTCTTACACCAGGAGAGGATGG PRIMER_RIGHT_623_SEQUENCE=TCATGCAAGTTGGTACTCTTCTCACT PRIMER_LEFT_623=600,24 PRIMER_RIGHT_623=699,26 PRIMER_LEFT_623_TM=61.855 PRIMER_RIGHT_623_TM=62.652 PRIMER_LEFT_623_GC_PERCENT=45.833 PRIMER_RIGHT_623_GC_PERCENT=42.308 PRIMER_LEFT_623_SELF_ANY=4.00 PRIMER_RIGHT_623_SELF_ANY=5.00 PRIMER_LEFT_623_SELF_END=0.00 PRIMER_RIGHT_623_SELF_END=1.00 PRIMER_LEFT_623_END_STABILITY=8.1000 PRIMER_RIGHT_623_END_STABILITY=6.4000 PRIMER_PAIR_623_COMPL_ANY=5.00 PRIMER_PAIR_623_COMPL_END=3.00 PRIMER_PAIR_623_PRODUCT_SIZE=100 PRIMER_PAIR_624_PENALTY=14.571495 PRIMER_LEFT_624_PENALTY=5.606786 PRIMER_RIGHT_624_PENALTY=8.964709 PRIMER_LEFT_624_SEQUENCE=ACAAGTGCAAATTCTTACACCAGG PRIMER_RIGHT_624_SEQUENCE=GTACTCTTCTCACTGCATGTCTCTTTG PRIMER_LEFT_624=591,24 PRIMER_RIGHT_624=687,27 PRIMER_LEFT_624_TM=61.607 PRIMER_RIGHT_624_TM=61.965 PRIMER_LEFT_624_GC_PERCENT=41.667 PRIMER_RIGHT_624_GC_PERCENT=44.444 PRIMER_LEFT_624_SELF_ANY=5.00 PRIMER_RIGHT_624_SELF_ANY=4.00 PRIMER_LEFT_624_SELF_END=3.00 PRIMER_RIGHT_624_SELF_END=0.00 PRIMER_LEFT_624_END_STABILITY=9.7000 PRIMER_RIGHT_624_END_STABILITY=7.3000 PRIMER_PAIR_624_COMPL_ANY=4.00 PRIMER_PAIR_624_COMPL_END=1.00 PRIMER_PAIR_624_PRODUCT_SIZE=97 PRIMER_PAIR_625_PENALTY=14.625553 PRIMER_LEFT_625_PENALTY=6.738568 PRIMER_RIGHT_625_PENALTY=7.886984 PRIMER_LEFT_625_SEQUENCE=GCTTAGTTGGTGAGTTTGTAAGTGAT PRIMER_RIGHT_625_SEQUENCE=CCATCCTCTCCTGGTGTAAGAATTT PRIMER_LEFT_625=549,26 PRIMER_RIGHT_625=623,25 PRIMER_LEFT_625_TM=59.261 PRIMER_RIGHT_625_TM=62.887 PRIMER_LEFT_625_GC_PERCENT=38.462 PRIMER_RIGHT_625_GC_PERCENT=44.000 PRIMER_LEFT_625_SELF_ANY=4.00 PRIMER_RIGHT_625_SELF_ANY=4.00 PRIMER_LEFT_625_SELF_END=2.00 PRIMER_RIGHT_625_SELF_END=3.00 PRIMER_LEFT_625_END_STABILITY=6.3000 PRIMER_RIGHT_625_END_STABILITY=7.2000 PRIMER_PAIR_625_COMPL_ANY=4.00 PRIMER_PAIR_625_COMPL_END=1.00 PRIMER_PAIR_625_PRODUCT_SIZE=75 PRIMER_PAIR_626_PENALTY=14.855695 PRIMER_LEFT_626_PENALTY=6.204160 PRIMER_RIGHT_626_PENALTY=8.651535 PRIMER_LEFT_626_SEQUENCE=GATGTTTGCGAAACTCATCTTCAC PRIMER_RIGHT_626_SEQUENCE=AGTCATGCAAGTTGGTACTCTTCTCA PRIMER_LEFT_626=623,24 PRIMER_RIGHT_626=701,26 PRIMER_LEFT_626_TM=62.204 PRIMER_RIGHT_626_TM=62.652 PRIMER_LEFT_626_GC_PERCENT=41.667 PRIMER_RIGHT_626_GC_PERCENT=42.308 PRIMER_LEFT_626_SELF_ANY=6.00 PRIMER_RIGHT_626_SELF_ANY=5.00 PRIMER_LEFT_626_SELF_END=3.00 PRIMER_RIGHT_626_SELF_END=1.00 PRIMER_LEFT_626_END_STABILITY=6.7000 PRIMER_RIGHT_626_END_STABILITY=6.7000 PRIMER_PAIR_626_COMPL_ANY=4.00 PRIMER_PAIR_626_COMPL_END=2.00 PRIMER_PAIR_626_PRODUCT_SIZE=79 PRIMER_PAIR_627_PENALTY=14.923350 PRIMER_LEFT_627_PENALTY=5.363671 PRIMER_RIGHT_627_PENALTY=9.559679 PRIMER_LEFT_627_SEQUENCE=AGTGCAAATTCTTACACCAGGAGA PRIMER_RIGHT_627_SEQUENCE=GGTACTCTTCTCACTGCATGTCTCTTT PRIMER_LEFT_627=594,24 PRIMER_RIGHT_627=688,27 PRIMER_LEFT_627_TM=61.364 PRIMER_RIGHT_627_TM=62.560 PRIMER_LEFT_627_GC_PERCENT=41.667 PRIMER_RIGHT_627_GC_PERCENT=44.444 PRIMER_LEFT_627_SELF_ANY=5.00 PRIMER_RIGHT_627_SELF_ANY=4.00 PRIMER_LEFT_627_SELF_END=1.00 PRIMER_RIGHT_627_SELF_END=0.00 PRIMER_LEFT_627_END_STABILITY=7.9000 PRIMER_RIGHT_627_END_STABILITY=7.0000 PRIMER_PAIR_627_COMPL_ANY=5.00 PRIMER_PAIR_627_COMPL_END=3.00 PRIMER_PAIR_627_PRODUCT_SIZE=95 PRIMER_PAIR_628_PENALTY=15.585840 PRIMER_LEFT_628_PENALTY=5.624627 PRIMER_RIGHT_628_PENALTY=9.961213 PRIMER_LEFT_628_SEQUENCE=AAATTCTTACACCAGGAGAGGAT PRIMER_RIGHT_628_SEQUENCE=AGTTGGTACTCTTCTCACTGCATGTCT PRIMER_LEFT_628=599,23 PRIMER_RIGHT_628=692,27 PRIMER_LEFT_628_TM=57.375 PRIMER_RIGHT_628_TM=62.961 PRIMER_LEFT_628_GC_PERCENT=39.130 PRIMER_RIGHT_628_GC_PERCENT=44.444 PRIMER_LEFT_628_SELF_ANY=4.00 PRIMER_RIGHT_628_SELF_ANY=4.00 PRIMER_LEFT_628_SELF_END=2.00 PRIMER_RIGHT_628_SELF_END=0.00 PRIMER_LEFT_628_END_STABILITY=7.8000 PRIMER_RIGHT_628_END_STABILITY=6.4000 PRIMER_PAIR_628_COMPL_ANY=6.00 PRIMER_PAIR_628_COMPL_END=3.00 PRIMER_PAIR_628_PRODUCT_SIZE=94 PRIMER_PAIR_629_PENALTY=16.356344 PRIMER_LEFT_629_PENALTY=6.781558 PRIMER_RIGHT_629_PENALTY=9.574785 PRIMER_LEFT_629_SEQUENCE=CTTACACCAGGAGAGGATGGATGT PRIMER_RIGHT_629_SEQUENCE=TAGTCATGCAAGTTGGTACTCTTCTCA PRIMER_LEFT_629=604,24 PRIMER_RIGHT_629=702,27 PRIMER_LEFT_629_TM=62.782 PRIMER_RIGHT_629_TM=62.575 PRIMER_LEFT_629_GC_PERCENT=50.000 PRIMER_RIGHT_629_GC_PERCENT=40.741 PRIMER_LEFT_629_SELF_ANY=4.00 PRIMER_RIGHT_629_SELF_ANY=5.00 PRIMER_LEFT_629_SELF_END=0.00 PRIMER_RIGHT_629_SELF_END=1.00 PRIMER_LEFT_629_END_STABILITY=6.3000 PRIMER_RIGHT_629_END_STABILITY=6.7000 PRIMER_PAIR_629_COMPL_ANY=5.00 PRIMER_PAIR_629_COMPL_END=1.00 PRIMER_PAIR_629_PRODUCT_SIZE=99 PRIMER_PAIR_630_PENALTY=2.436932 PRIMER_LEFT_630_PENALTY=2.199316 PRIMER_RIGHT_630_PENALTY=0.237616 PRIMER_LEFT_630_SEQUENCE=GGGCTCCGTCAGTTTCCT PRIMER_RIGHT_630_SEQUENCE=ACCATCAGTGGGTACCTCCA PRIMER_LEFT_630=43,18 PRIMER_RIGHT_630=271,20 PRIMER_LEFT_630_TM=60.199 PRIMER_RIGHT_630_TM=60.238 PRIMER_LEFT_630_GC_PERCENT=61.111 PRIMER_RIGHT_630_GC_PERCENT=55.000 PRIMER_LEFT_630_SELF_ANY=2.00 PRIMER_RIGHT_630_SELF_ANY=6.00 PRIMER_LEFT_630_SELF_END=0.00 PRIMER_RIGHT_630_SELF_END=2.00 PRIMER_LEFT_630_END_STABILITY=8.2000 PRIMER_RIGHT_630_END_STABILITY=8.2000 PRIMER_PAIR_630_COMPL_ANY=3.00 PRIMER_PAIR_630_COMPL_END=1.00 PRIMER_PAIR_630_PRODUCT_SIZE=229 PRIMER_PAIR_631_PENALTY=2.811275 PRIMER_LEFT_631_PENALTY=2.739036 PRIMER_RIGHT_631_PENALTY=0.072238 PRIMER_LEFT_631_SEQUENCE=AAACTCATCTTCACTGGCACAC PRIMER_RIGHT_631_SEQUENCE=CCAGACATCCGAGTCATCCT PRIMER_LEFT_631=633,22 PRIMER_RIGHT_631=826,20 PRIMER_LEFT_631_TM=59.261 PRIMER_RIGHT_631_TM=60.072 PRIMER_LEFT_631_GC_PERCENT=45.455 PRIMER_RIGHT_631_GC_PERCENT=55.000 PRIMER_LEFT_631_SELF_ANY=3.00 PRIMER_RIGHT_631_SELF_ANY=5.00 PRIMER_LEFT_631_SELF_END=0.00 PRIMER_RIGHT_631_SELF_END=0.00 PRIMER_LEFT_631_END_STABILITY=6.4000 PRIMER_RIGHT_631_END_STABILITY=7.8000 PRIMER_PAIR_631_COMPL_ANY=4.00 PRIMER_PAIR_631_COMPL_END=0.00 PRIMER_PAIR_631_PRODUCT_SIZE=194 PRIMER_PAIR_632_PENALTY=3.027861 PRIMER_LEFT_632_PENALTY=2.495254 PRIMER_RIGHT_632_PENALTY=0.532607 PRIMER_LEFT_632_SEQUENCE=GTCAGTTTCCTCGGCAGC PRIMER_RIGHT_632_SEQUENCE=CATCAGTGGGTACCTCCAGC PRIMER_LEFT_632=50,18 PRIMER_RIGHT_632=269,20 PRIMER_LEFT_632_TM=59.505 PRIMER_RIGHT_632_TM=60.533 PRIMER_LEFT_632_GC_PERCENT=61.111 PRIMER_RIGHT_632_GC_PERCENT=60.000 PRIMER_LEFT_632_SELF_ANY=3.00 PRIMER_RIGHT_632_SELF_ANY=6.00 PRIMER_LEFT_632_SELF_END=3.00 PRIMER_RIGHT_632_SELF_END=2.00 PRIMER_LEFT_632_END_STABILITY=9.7000 PRIMER_RIGHT_632_END_STABILITY=9.7000 PRIMER_PAIR_632_COMPL_ANY=3.00 PRIMER_PAIR_632_COMPL_END=2.00 PRIMER_PAIR_632_PRODUCT_SIZE=220 PRIMER_PAIR_633_PENALTY=3.140197 PRIMER_LEFT_633_PENALTY=3.028072 PRIMER_RIGHT_633_PENALTY=0.112124 PRIMER_LEFT_633_SEQUENCE=ATCCCACTCGCACAGCAG PRIMER_RIGHT_633_SEQUENCE=CACAGAACATGGCAATCTGG PRIMER_LEFT_633=154,18 PRIMER_RIGHT_633=314,20 PRIMER_LEFT_633_TM=61.028 PRIMER_RIGHT_633_TM=60.112 PRIMER_LEFT_633_GC_PERCENT=61.111 PRIMER_RIGHT_633_GC_PERCENT=50.000 PRIMER_LEFT_633_SELF_ANY=2.00 PRIMER_RIGHT_633_SELF_ANY=4.00 PRIMER_LEFT_633_SELF_END=2.00 PRIMER_RIGHT_633_SELF_END=0.00 PRIMER_LEFT_633_END_STABILITY=8.2000 PRIMER_RIGHT_633_END_STABILITY=8.2000 PRIMER_PAIR_633_COMPL_ANY=3.00 PRIMER_PAIR_633_COMPL_END=2.00 PRIMER_PAIR_633_PRODUCT_SIZE=161 PRIMER_PAIR_634_PENALTY=3.288557 PRIMER_LEFT_634_PENALTY=3.216208 PRIMER_RIGHT_634_PENALTY=0.072349 PRIMER_LEFT_634_SEQUENCE=AACTCATCTTCACTGGCACAC PRIMER_RIGHT_634_SEQUENCE=ACCAGACATCCGAGTCATCC PRIMER_LEFT_634=634,21 PRIMER_RIGHT_634=827,20 PRIMER_LEFT_634_TM=57.784 PRIMER_RIGHT_634_TM=59.928 PRIMER_LEFT_634_GC_PERCENT=47.619 PRIMER_RIGHT_634_GC_PERCENT=55.000 PRIMER_LEFT_634_SELF_ANY=3.00 PRIMER_RIGHT_634_SELF_ANY=5.00 PRIMER_LEFT_634_SELF_END=0.00 PRIMER_RIGHT_634_SELF_END=0.00 PRIMER_LEFT_634_END_STABILITY=6.4000 PRIMER_RIGHT_634_END_STABILITY=8.1000 PRIMER_PAIR_634_COMPL_ANY=4.00 PRIMER_PAIR_634_COMPL_END=1.00 PRIMER_PAIR_634_PRODUCT_SIZE=194 PRIMER_PAIR_635_PENALTY=3.671011 PRIMER_LEFT_635_PENALTY=3.312402 PRIMER_RIGHT_635_PENALTY=0.358609 PRIMER_LEFT_635_SEQUENCE=GTTCCTGACAAGTGCAAATTCTT PRIMER_RIGHT_635_SEQUENCE=CAGTGGGCAACACACAAACT PRIMER_LEFT_635=584,23 PRIMER_RIGHT_635=766,20 PRIMER_LEFT_635_TM=59.688 PRIMER_RIGHT_635_TM=59.641 PRIMER_LEFT_635_GC_PERCENT=39.130 PRIMER_RIGHT_635_GC_PERCENT=50.000 PRIMER_LEFT_635_SELF_ANY=4.00 PRIMER_RIGHT_635_SELF_ANY=3.00 PRIMER_LEFT_635_SELF_END=1.00 PRIMER_RIGHT_635_SELF_END=1.00 PRIMER_LEFT_635_END_STABILITY=7.0000 PRIMER_RIGHT_635_END_STABILITY=6.7000 PRIMER_PAIR_635_COMPL_ANY=3.00 PRIMER_PAIR_635_COMPL_END=1.00 PRIMER_PAIR_635_PRODUCT_SIZE=183 PRIMER_PAIR_636_PENALTY=3.740295 PRIMER_LEFT_636_PENALTY=2.500904 PRIMER_RIGHT_636_PENALTY=1.239392 PRIMER_LEFT_636_SEQUENCE=GCTCCGTCAGTTTCCTCG PRIMER_RIGHT_636_SEQUENCE=TACCATCAGTGGGTACCTCCA PRIMER_LEFT_636=45,18 PRIMER_RIGHT_636=272,21 PRIMER_LEFT_636_TM=59.499 PRIMER_RIGHT_636_TM=60.239 PRIMER_LEFT_636_GC_PERCENT=61.111 PRIMER_RIGHT_636_GC_PERCENT=52.381 PRIMER_LEFT_636_SELF_ANY=2.00 PRIMER_RIGHT_636_SELF_ANY=6.00 PRIMER_LEFT_636_SELF_END=2.00 PRIMER_RIGHT_636_SELF_END=2.00 PRIMER_LEFT_636_END_STABILITY=9.9000 PRIMER_RIGHT_636_END_STABILITY=8.2000 PRIMER_PAIR_636_COMPL_ANY=3.00 PRIMER_PAIR_636_COMPL_END=1.00 PRIMER_PAIR_636_PRODUCT_SIZE=228 PRIMER_PAIR_637_PENALTY=3.863012 PRIMER_LEFT_637_PENALTY=2.546814 PRIMER_RIGHT_637_PENALTY=1.316198 PRIMER_LEFT_637_SEQUENCE=CGTCAGTTTCCTCGGCAG PRIMER_RIGHT_637_SEQUENCE=CCAGCATTACCATCAGTGGG PRIMER_LEFT_637=49,18 PRIMER_RIGHT_637=279,20 PRIMER_LEFT_637_TM=60.547 PRIMER_RIGHT_637_TM=61.316 PRIMER_LEFT_637_GC_PERCENT=61.111 PRIMER_RIGHT_637_GC_PERCENT=55.000 PRIMER_LEFT_637_SELF_ANY=2.00 PRIMER_RIGHT_637_SELF_ANY=3.00 PRIMER_LEFT_637_SELF_END=2.00 PRIMER_RIGHT_637_SELF_END=1.00 PRIMER_LEFT_637_END_STABILITY=9.7000 PRIMER_RIGHT_637_END_STABILITY=9.4000 PRIMER_PAIR_637_COMPL_ANY=2.00 PRIMER_PAIR_637_COMPL_END=0.00 PRIMER_PAIR_637_PRODUCT_SIZE=231 PRIMER_PAIR_638_PENALTY=4.133102 PRIMER_LEFT_638_PENALTY=2.664693 PRIMER_RIGHT_638_PENALTY=1.468408 PRIMER_LEFT_638_SEQUENCE=CTCCGTCAGTTTCCTCGG PRIMER_RIGHT_638_SEQUENCE=GCCAGCATTACCATCAGTGG PRIMER_LEFT_638=46,18 PRIMER_RIGHT_638=280,20 PRIMER_LEFT_638_TM=59.335 PRIMER_RIGHT_638_TM=61.468 PRIMER_LEFT_638_GC_PERCENT=61.111 PRIMER_RIGHT_638_GC_PERCENT=55.000 PRIMER_LEFT_638_SELF_ANY=3.00 PRIMER_RIGHT_638_SELF_ANY=3.00 PRIMER_LEFT_638_SELF_END=2.00 PRIMER_RIGHT_638_SELF_END=3.00 PRIMER_LEFT_638_END_STABILITY=9.9000 PRIMER_RIGHT_638_END_STABILITY=7.9000 PRIMER_PAIR_638_COMPL_ANY=2.00 PRIMER_PAIR_638_COMPL_END=2.00 PRIMER_PAIR_638_PRODUCT_SIZE=235 PRIMER_PAIR_639_PENALTY=4.203374 PRIMER_LEFT_639_PENALTY=4.091249 PRIMER_RIGHT_639_PENALTY=0.112124 PRIMER_LEFT_639_SEQUENCE=GATCCCACTCGCACAGCA PRIMER_RIGHT_639_SEQUENCE=CCACAGAACATGGCAATCTG PRIMER_LEFT_639=153,18 PRIMER_RIGHT_639=315,20 PRIMER_LEFT_639_TM=62.091 PRIMER_RIGHT_639_TM=60.112 PRIMER_LEFT_639_GC_PERCENT=61.111 PRIMER_RIGHT_639_GC_PERCENT=50.000 PRIMER_LEFT_639_SELF_ANY=4.00 PRIMER_RIGHT_639_SELF_ANY=4.00 PRIMER_LEFT_639_SELF_END=0.00 PRIMER_RIGHT_639_SELF_END=2.00 PRIMER_LEFT_639_END_STABILITY=8.5000 PRIMER_RIGHT_639_END_STABILITY=6.6000 PRIMER_PAIR_639_COMPL_ANY=3.00 PRIMER_PAIR_639_COMPL_END=2.00 PRIMER_PAIR_639_PRODUCT_SIZE=163 PRIMER_PAIR_640_PENALTY=4.215026 PRIMER_LEFT_640_PENALTY=3.270687 PRIMER_RIGHT_640_PENALTY=0.944339 PRIMER_LEFT_640_SEQUENCE=CTTCACTGGCACACCGTC PRIMER_RIGHT_640_SEQUENCE=ACATCCGAGTCATCCTCCTC PRIMER_LEFT_640=641,18 PRIMER_RIGHT_640=822,20 PRIMER_LEFT_640_TM=58.729 PRIMER_RIGHT_640_TM=59.056 PRIMER_LEFT_640_GC_PERCENT=61.111 PRIMER_RIGHT_640_GC_PERCENT=55.000 PRIMER_LEFT_640_SELF_ANY=3.00 PRIMER_RIGHT_640_SELF_ANY=3.00 PRIMER_LEFT_640_SELF_END=1.00 PRIMER_RIGHT_640_SELF_END=0.00 PRIMER_LEFT_640_END_STABILITY=9.6000 PRIMER_RIGHT_640_END_STABILITY=7.9000 PRIMER_PAIR_640_COMPL_ANY=3.00 PRIMER_PAIR_640_COMPL_END=0.00 PRIMER_PAIR_640_PRODUCT_SIZE=182 PRIMER_PAIR_641_PENALTY=4.215026 PRIMER_LEFT_641_PENALTY=3.270687 PRIMER_RIGHT_641_PENALTY=0.944339 PRIMER_LEFT_641_SEQUENCE=TCTTCACTGGCACACCGT PRIMER_RIGHT_641_SEQUENCE=GACATCCGAGTCATCCTCCT PRIMER_LEFT_641=640,18 PRIMER_RIGHT_641=823,20 PRIMER_LEFT_641_TM=58.729 PRIMER_RIGHT_641_TM=59.056 PRIMER_LEFT_641_GC_PERCENT=55.556 PRIMER_RIGHT_641_GC_PERCENT=55.000 PRIMER_LEFT_641_SELF_ANY=3.00 PRIMER_RIGHT_641_SELF_ANY=5.00 PRIMER_LEFT_641_SELF_END=3.00 PRIMER_RIGHT_641_SELF_END=0.00 PRIMER_LEFT_641_END_STABILITY=9.3000 PRIMER_RIGHT_641_END_STABILITY=7.9000 PRIMER_PAIR_641_COMPL_ANY=3.00 PRIMER_PAIR_641_COMPL_END=0.00 PRIMER_PAIR_641_PRODUCT_SIZE=184 PRIMER_PAIR_642_PENALTY=4.354791 PRIMER_LEFT_642_PENALTY=3.532432 PRIMER_RIGHT_642_PENALTY=0.822359 PRIMER_LEFT_642_SEQUENCE=TTCCTGACAAGTGCAAATTCT PRIMER_RIGHT_642_SEQUENCE=CACTTTCTTCAGCCAGTGGG PRIMER_LEFT_642=585,21 PRIMER_RIGHT_642=779,20 PRIMER_LEFT_642_TM=57.468 PRIMER_RIGHT_642_TM=60.822 PRIMER_LEFT_642_GC_PERCENT=38.095 PRIMER_RIGHT_642_GC_PERCENT=55.000 PRIMER_LEFT_642_SELF_ANY=4.00 PRIMER_RIGHT_642_SELF_ANY=6.00 PRIMER_LEFT_642_SELF_END=2.00 PRIMER_RIGHT_642_SELF_END=3.00 PRIMER_LEFT_642_END_STABILITY=6.6000 PRIMER_RIGHT_642_END_STABILITY=9.4000 PRIMER_PAIR_642_COMPL_ANY=5.00 PRIMER_PAIR_642_COMPL_END=0.00 PRIMER_PAIR_642_PRODUCT_SIZE=195 PRIMER_PAIR_643_PENALTY=4.455354 PRIMER_LEFT_643_PENALTY=3.511015 PRIMER_RIGHT_643_PENALTY=0.944339 PRIMER_LEFT_643_SEQUENCE=AACTCATCTTCACTGGCACACC PRIMER_RIGHT_643_SEQUENCE=AGACATCCGAGTCATCCTCC PRIMER_LEFT_643=634,22 PRIMER_RIGHT_643=824,20 PRIMER_LEFT_643_TM=61.511 PRIMER_RIGHT_643_TM=59.056 PRIMER_LEFT_643_GC_PERCENT=50.000 PRIMER_RIGHT_643_GC_PERCENT=55.000 PRIMER_LEFT_643_SELF_ANY=3.00 PRIMER_RIGHT_643_SELF_ANY=5.00 PRIMER_LEFT_643_SELF_END=0.00 PRIMER_RIGHT_643_SELF_END=0.00 PRIMER_LEFT_643_END_STABILITY=7.6000 PRIMER_RIGHT_643_END_STABILITY=9.4000 PRIMER_PAIR_643_COMPL_ANY=4.00 PRIMER_PAIR_643_COMPL_END=0.00 PRIMER_PAIR_643_PRODUCT_SIZE=191 PRIMER_PAIR_644_PENALTY=4.531863 PRIMER_LEFT_644_PENALTY=3.581573 PRIMER_RIGHT_644_PENALTY=0.950290 PRIMER_LEFT_644_SEQUENCE=TCTCGTTCCTGACAAGTGC PRIMER_RIGHT_644_SEQUENCE=CACCAGACATCCGAGTCATC PRIMER_LEFT_644=580,19 PRIMER_RIGHT_644=828,20 PRIMER_LEFT_644_TM=57.418 PRIMER_RIGHT_644_TM=59.050 PRIMER_LEFT_644_GC_PERCENT=52.632 PRIMER_RIGHT_644_GC_PERCENT=55.000 PRIMER_LEFT_644_SELF_ANY=3.00 PRIMER_RIGHT_644_SELF_ANY=5.00 PRIMER_LEFT_644_SELF_END=2.00 PRIMER_RIGHT_644_SELF_END=1.00 PRIMER_LEFT_644_END_STABILITY=7.9000 PRIMER_RIGHT_644_END_STABILITY=6.6000 PRIMER_PAIR_644_COMPL_ANY=4.00 PRIMER_PAIR_644_COMPL_END=0.00 PRIMER_PAIR_644_PRODUCT_SIZE=249 PRIMER_PAIR_645_PENALTY=4.585719 PRIMER_LEFT_645_PENALTY=3.629805 PRIMER_RIGHT_645_PENALTY=0.955914 PRIMER_LEFT_645_SEQUENCE=GTTCCTGACAAGTGCAAATTCT PRIMER_RIGHT_645_SEQUENCE=CCACCAGACATCCGAGTCAT PRIMER_LEFT_645=584,22 PRIMER_RIGHT_645=829,20 PRIMER_LEFT_645_TM=58.370 PRIMER_RIGHT_645_TM=60.956 PRIMER_LEFT_645_GC_PERCENT=40.909 PRIMER_RIGHT_645_GC_PERCENT=55.000 PRIMER_LEFT_645_SELF_ANY=4.00 PRIMER_RIGHT_645_SELF_ANY=5.00 PRIMER_LEFT_645_SELF_END=2.00 PRIMER_RIGHT_645_SELF_END=3.00 PRIMER_LEFT_645_END_STABILITY=6.6000 PRIMER_RIGHT_645_END_STABILITY=6.3000 PRIMER_PAIR_645_COMPL_ANY=4.00 PRIMER_PAIR_645_COMPL_END=1.00 PRIMER_PAIR_645_PRODUCT_SIZE=246 PRIMER_PAIR_646_PENALTY=4.593173 PRIMER_LEFT_646_PENALTY=3.124765 PRIMER_RIGHT_646_PENALTY=1.468408 PRIMER_LEFT_646_SEQUENCE=GGGCTCCGTCAGTTTCCTC PRIMER_RIGHT_646_SEQUENCE=GGCCAGCATTACCATCAGTG PRIMER_LEFT_646=43,19 PRIMER_RIGHT_646=281,20 PRIMER_LEFT_646_TM=62.125 PRIMER_RIGHT_646_TM=61.468 PRIMER_LEFT_646_GC_PERCENT=63.158 PRIMER_RIGHT_646_GC_PERCENT=55.000 PRIMER_LEFT_646_SELF_ANY=2.00 PRIMER_RIGHT_646_SELF_ANY=4.00 PRIMER_LEFT_646_SELF_END=0.00 PRIMER_RIGHT_646_SELF_END=3.00 PRIMER_LEFT_646_END_STABILITY=7.9000 PRIMER_RIGHT_646_END_STABILITY=6.7000 PRIMER_PAIR_646_COMPL_ANY=3.00 PRIMER_PAIR_646_COMPL_END=1.00 PRIMER_PAIR_646_PRODUCT_SIZE=239 PRIMER_PAIR_647_PENALTY=4.607356 PRIMER_LEFT_647_PENALTY=3.683571 PRIMER_RIGHT_647_PENALTY=0.923785 PRIMER_LEFT_647_SEQUENCE=AGCGGTAGGCGAGAGCAC PRIMER_RIGHT_647_SEQUENCE=ACAGAACATGGCAATCTGGG PRIMER_LEFT_647=65,18 PRIMER_RIGHT_647=313,20 PRIMER_LEFT_647_TM=61.684 PRIMER_RIGHT_647_TM=60.924 PRIMER_LEFT_647_GC_PERCENT=66.667 PRIMER_RIGHT_647_GC_PERCENT=50.000 PRIMER_LEFT_647_SELF_ANY=2.00 PRIMER_RIGHT_647_SELF_ANY=4.00 PRIMER_LEFT_647_SELF_END=2.00 PRIMER_RIGHT_647_SELF_END=0.00 PRIMER_LEFT_647_END_STABILITY=7.9000 PRIMER_RIGHT_647_END_STABILITY=9.7000 PRIMER_PAIR_647_COMPL_ANY=3.00 PRIMER_PAIR_647_COMPL_END=1.00 PRIMER_PAIR_647_PRODUCT_SIZE=249 PRIMER_PAIR_648_PENALTY=4.721478 PRIMER_LEFT_648_PENALTY=3.633158 PRIMER_RIGHT_648_PENALTY=1.088320 PRIMER_LEFT_648_SEQUENCE=GAAACTCATCTTCACTGGCAC PRIMER_RIGHT_648_SEQUENCE=AGACATCCGAGTCATCCTCCT PRIMER_LEFT_648=632,21 PRIMER_RIGHT_648=824,21 PRIMER_LEFT_648_TM=57.367 PRIMER_RIGHT_648_TM=60.088 PRIMER_LEFT_648_GC_PERCENT=47.619 PRIMER_RIGHT_648_GC_PERCENT=52.381 PRIMER_LEFT_648_SELF_ANY=3.00 PRIMER_RIGHT_648_SELF_ANY=5.00 PRIMER_LEFT_648_SELF_END=1.00 PRIMER_RIGHT_648_SELF_END=0.00 PRIMER_LEFT_648_END_STABILITY=9.4000 PRIMER_RIGHT_648_END_STABILITY=7.9000 PRIMER_PAIR_648_COMPL_ANY=4.00 PRIMER_PAIR_648_COMPL_END=0.00 PRIMER_PAIR_648_PRODUCT_SIZE=193 PRIMER_PAIR_649_PENALTY=4.772239 PRIMER_LEFT_649_PENALTY=2.325240 PRIMER_RIGHT_649_PENALTY=2.446999 PRIMER_LEFT_649_SEQUENCE=GCACATGAATGTCCAGAATGG PRIMER_RIGHT_649_SEQUENCE=GTCACTGGTTGGTTGGCTT PRIMER_LEFT_649=328,21 PRIMER_RIGHT_649=477,19 PRIMER_LEFT_649_TM=61.325 PRIMER_RIGHT_649_TM=58.553 PRIMER_LEFT_649_GC_PERCENT=47.619 PRIMER_RIGHT_649_GC_PERCENT=52.632 PRIMER_LEFT_649_SELF_ANY=6.00 PRIMER_RIGHT_649_SELF_ANY=3.00 PRIMER_LEFT_649_SELF_END=3.00 PRIMER_RIGHT_649_SELF_END=0.00 PRIMER_LEFT_649_END_STABILITY=8.4000 PRIMER_RIGHT_649_END_STABILITY=9.7000 PRIMER_PAIR_649_COMPL_ANY=4.00 PRIMER_PAIR_649_COMPL_END=0.00 PRIMER_PAIR_649_PRODUCT_SIZE=150 PRIMER_PAIR_650_PENALTY=4.865284 PRIMER_LEFT_650_PENALTY=3.688912 PRIMER_RIGHT_650_PENALTY=1.176371 PRIMER_LEFT_650_SEQUENCE=GTTCCTGACAAGTGCAAATTC PRIMER_RIGHT_650_SEQUENCE=AGTCATCCTCCTCCGCATC PRIMER_LEFT_650=584,21 PRIMER_RIGHT_650=815,19 PRIMER_LEFT_650_TM=57.311 PRIMER_RIGHT_650_TM=60.176 PRIMER_LEFT_650_GC_PERCENT=42.857 PRIMER_RIGHT_650_GC_PERCENT=57.895 PRIMER_LEFT_650_SELF_ANY=4.00 PRIMER_RIGHT_650_SELF_ANY=2.00 PRIMER_LEFT_650_SELF_END=2.00 PRIMER_RIGHT_650_SELF_END=0.00 PRIMER_LEFT_650_END_STABILITY=6.9000 PRIMER_RIGHT_650_END_STABILITY=8.1000 PRIMER_PAIR_650_COMPL_ANY=4.00 PRIMER_PAIR_650_COMPL_END=1.00 PRIMER_PAIR_650_PRODUCT_SIZE=232 PRIMER_PAIR_651_PENALTY=4.870781 PRIMER_LEFT_651_PENALTY=3.694409 PRIMER_RIGHT_651_PENALTY=1.176371 PRIMER_LEFT_651_SEQUENCE=AAGTGATGCCCTTCTCGTT PRIMER_RIGHT_651_SEQUENCE=GAGTCATCCTCCTCCGCAT PRIMER_LEFT_651=568,19 PRIMER_RIGHT_651=816,19 PRIMER_LEFT_651_TM=57.306 PRIMER_RIGHT_651_TM=60.176 PRIMER_LEFT_651_GC_PERCENT=47.368 PRIMER_RIGHT_651_GC_PERCENT=57.895 PRIMER_LEFT_651_SELF_ANY=4.00 PRIMER_RIGHT_651_SELF_ANY=3.00 PRIMER_LEFT_651_SELF_END=2.00 PRIMER_RIGHT_651_SELF_END=2.00 PRIMER_LEFT_651_END_STABILITY=8.4000 PRIMER_RIGHT_651_END_STABILITY=10.1000 PRIMER_PAIR_651_COMPL_ANY=4.00 PRIMER_PAIR_651_COMPL_END=0.00 PRIMER_PAIR_651_PRODUCT_SIZE=249 PRIMER_PAIR_652_PENALTY=4.919996 PRIMER_LEFT_652_PENALTY=3.362783 PRIMER_RIGHT_652_PENALTY=1.557214 PRIMER_LEFT_652_SEQUENCE=CTCGGGCTCCGTCAGTTT PRIMER_RIGHT_652_SEQUENCE=CAGCATTACCATCAGTGGGT PRIMER_LEFT_652=40,18 PRIMER_RIGHT_652=278,20 PRIMER_LEFT_652_TM=61.363 PRIMER_RIGHT_652_TM=58.443 PRIMER_LEFT_652_GC_PERCENT=61.111 PRIMER_RIGHT_652_GC_PERCENT=50.000 PRIMER_LEFT_652_SELF_ANY=4.00 PRIMER_RIGHT_652_SELF_ANY=3.00 PRIMER_LEFT_652_SELF_END=0.00 PRIMER_RIGHT_652_SELF_END=3.00 PRIMER_LEFT_652_END_STABILITY=6.7000 PRIMER_RIGHT_652_END_STABILITY=9.4000 PRIMER_PAIR_652_COMPL_ANY=3.00 PRIMER_PAIR_652_COMPL_END=0.00 PRIMER_PAIR_652_PRODUCT_SIZE=239 PRIMER_PAIR_653_PENALTY=5.030515 PRIMER_LEFT_653_PENALTY=3.774565 PRIMER_RIGHT_653_PENALTY=1.255950 PRIMER_LEFT_653_SEQUENCE=AAGTGATGCCCTTCTCGTTCC PRIMER_RIGHT_653_SEQUENCE=TCAGCAGAATCCACATTGTCA PRIMER_LEFT_653=568,21 PRIMER_RIGHT_653=798,21 PRIMER_LEFT_653_TM=62.775 PRIMER_RIGHT_653_TM=60.256 PRIMER_LEFT_653_GC_PERCENT=52.381 PRIMER_RIGHT_653_GC_PERCENT=42.857 PRIMER_LEFT_653_SELF_ANY=4.00 PRIMER_RIGHT_653_SELF_ANY=5.00 PRIMER_LEFT_653_SELF_END=0.00 PRIMER_RIGHT_653_SELF_END=3.00 PRIMER_LEFT_653_END_STABILITY=7.9000 PRIMER_RIGHT_653_END_STABILITY=6.7000 PRIMER_PAIR_653_COMPL_ANY=4.00 PRIMER_PAIR_653_COMPL_END=1.00 PRIMER_PAIR_653_PRODUCT_SIZE=231 PRIMER_PAIR_654_PENALTY=5.034918 PRIMER_LEFT_654_PENALTY=3.708825 PRIMER_RIGHT_654_PENALTY=1.326093 PRIMER_LEFT_654_SEQUENCE=GCCCTTCTCGTTCCTGAC PRIMER_RIGHT_654_SEQUENCE=GCATCAGCAGAATCCACATT PRIMER_LEFT_654=575,18 PRIMER_RIGHT_654=801,20 PRIMER_LEFT_654_TM=58.291 PRIMER_RIGHT_654_TM=58.674 PRIMER_LEFT_654_GC_PERCENT=61.111 PRIMER_RIGHT_654_GC_PERCENT=45.000 PRIMER_LEFT_654_SELF_ANY=2.00 PRIMER_RIGHT_654_SELF_ANY=4.00 PRIMER_LEFT_654_SELF_END=1.00 PRIMER_RIGHT_654_SELF_END=2.00 PRIMER_LEFT_654_END_STABILITY=6.4000 PRIMER_RIGHT_654_END_STABILITY=6.6000 PRIMER_PAIR_654_COMPL_ANY=4.00 PRIMER_PAIR_654_COMPL_END=0.00 PRIMER_PAIR_654_PRODUCT_SIZE=227 PRIMER_PAIR_655_PENALTY=5.056684 PRIMER_LEFT_655_PENALTY=2.561455 PRIMER_RIGHT_655_PENALTY=2.495229 PRIMER_LEFT_655_SEQUENCE=GCACATGAATGTCCAGAATG PRIMER_RIGHT_655_SEQUENCE=GTCACTGGTTGGTTGGCTTCT PRIMER_LEFT_655=328,20 PRIMER_RIGHT_655=477,21 PRIMER_LEFT_655_TM=57.439 PRIMER_RIGHT_655_TM=61.495 PRIMER_LEFT_655_GC_PERCENT=45.000 PRIMER_RIGHT_655_GC_PERCENT=52.381 PRIMER_LEFT_655_SELF_ANY=6.00 PRIMER_RIGHT_655_SELF_ANY=3.00 PRIMER_LEFT_655_SELF_END=1.00 PRIMER_RIGHT_655_SELF_END=0.00 PRIMER_LEFT_655_END_STABILITY=6.9000 PRIMER_RIGHT_655_END_STABILITY=6.7000 PRIMER_PAIR_655_COMPL_ANY=4.00 PRIMER_PAIR_655_COMPL_END=3.00 PRIMER_PAIR_655_PRODUCT_SIZE=150 PRIMER_PAIR_656_PENALTY=5.090283 PRIMER_LEFT_656_PENALTY=3.892438 PRIMER_RIGHT_656_PENALTY=1.197845 PRIMER_LEFT_656_SEQUENCE=ATCTTCACTGGCACACCG PRIMER_RIGHT_656_SEQUENCE=TTGTCTTCACTCCCATCTGC PRIMER_LEFT_656=639,18 PRIMER_RIGHT_656=870,20 PRIMER_LEFT_656_TM=58.108 PRIMER_RIGHT_656_TM=58.802 PRIMER_LEFT_656_GC_PERCENT=55.556 PRIMER_RIGHT_656_GC_PERCENT=50.000 PRIMER_LEFT_656_SELF_ANY=3.00 PRIMER_RIGHT_656_SELF_ANY=2.00 PRIMER_LEFT_656_SELF_END=2.00 PRIMER_RIGHT_656_SELF_END=2.00 PRIMER_LEFT_656_END_STABILITY=9.9000 PRIMER_RIGHT_656_END_STABILITY=8.2000 PRIMER_PAIR_656_COMPL_ANY=3.00 PRIMER_PAIR_656_COMPL_END=2.00 PRIMER_PAIR_656_PRODUCT_SIZE=232 PRIMER_PAIR_657_PENALTY=5.306162 PRIMER_LEFT_657_PENALTY=3.928601 PRIMER_RIGHT_657_PENALTY=1.377561 PRIMER_LEFT_657_SEQUENCE=CGTTCCTGACAAGTGCAAATTC PRIMER_RIGHT_657_SEQUENCE=AGTGGGCAACACACAAACTC PRIMER_LEFT_657=583,22 PRIMER_RIGHT_657=765,20 PRIMER_LEFT_657_TM=61.929 PRIMER_RIGHT_657_TM=58.622 PRIMER_LEFT_657_GC_PERCENT=45.455 PRIMER_RIGHT_657_GC_PERCENT=50.000 PRIMER_LEFT_657_SELF_ANY=4.00 PRIMER_RIGHT_657_SELF_ANY=3.00 PRIMER_LEFT_657_SELF_END=2.00 PRIMER_RIGHT_657_SELF_END=0.00 PRIMER_LEFT_657_END_STABILITY=6.9000 PRIMER_RIGHT_657_END_STABILITY=6.4000 PRIMER_PAIR_657_COMPL_ANY=3.00 PRIMER_PAIR_657_COMPL_END=0.00 PRIMER_PAIR_657_PRODUCT_SIZE=183 PRIMER_PAIR_658_PENALTY=5.345528 PRIMER_LEFT_658_PENALTY=3.708825 PRIMER_RIGHT_658_PENALTY=1.636702 PRIMER_LEFT_658_SEQUENCE=GGCTCCGTCAGTTTCCTC PRIMER_RIGHT_658_SEQUENCE=CAGGCCAGCATTACCATCA PRIMER_LEFT_658=44,18 PRIMER_RIGHT_658=283,19 PRIMER_LEFT_658_TM=58.291 PRIMER_RIGHT_658_TM=60.637 PRIMER_LEFT_658_GC_PERCENT=61.111 PRIMER_RIGHT_658_GC_PERCENT=52.632 PRIMER_LEFT_658_SELF_ANY=2.00 PRIMER_RIGHT_658_SELF_ANY=4.00 PRIMER_LEFT_658_SELF_END=0.00 PRIMER_RIGHT_658_SELF_END=1.00 PRIMER_LEFT_658_END_STABILITY=7.9000 PRIMER_RIGHT_658_END_STABILITY=6.9000 PRIMER_PAIR_658_COMPL_ANY=3.00 PRIMER_PAIR_658_COMPL_END=0.00 PRIMER_PAIR_658_PRODUCT_SIZE=240 PRIMER_PAIR_659_PENALTY=5.348027 PRIMER_LEFT_659_PENALTY=4.100461 PRIMER_RIGHT_659_PENALTY=1.247566 PRIMER_LEFT_659_SEQUENCE=GAAACTCATCTTCACTGGCACAC PRIMER_RIGHT_659_SEQUENCE=TTTGTCTTCACTCCCATCTGC PRIMER_LEFT_659=632,23 PRIMER_RIGHT_659=871,21 PRIMER_LEFT_659_TM=61.100 PRIMER_RIGHT_659_TM=60.248 PRIMER_LEFT_659_GC_PERCENT=47.826 PRIMER_RIGHT_659_GC_PERCENT=47.619 PRIMER_LEFT_659_SELF_ANY=3.00 PRIMER_RIGHT_659_SELF_ANY=2.00 PRIMER_LEFT_659_SELF_END=0.00 PRIMER_RIGHT_659_SELF_END=2.00 PRIMER_LEFT_659_END_STABILITY=6.4000 PRIMER_RIGHT_659_END_STABILITY=8.2000 PRIMER_PAIR_659_COMPL_ANY=3.00 PRIMER_PAIR_659_COMPL_END=2.00 PRIMER_PAIR_659_PRODUCT_SIZE=240 PRIMER_PAIR_660_PENALTY=5.355960 PRIMER_LEFT_660_PENALTY=2.150151 PRIMER_RIGHT_660_PENALTY=3.205809 PRIMER_LEFT_660_SEQUENCE=ATGAATGTCCAGAATGGGAAG PRIMER_RIGHT_660_SEQUENCE=ACTCACCAACTAAGCAGCGGTA PRIMER_LEFT_660=332,21 PRIMER_RIGHT_660=563,22 PRIMER_LEFT_660_TM=58.850 PRIMER_RIGHT_660_TM=61.206 PRIMER_LEFT_660_GC_PERCENT=42.857 PRIMER_RIGHT_660_GC_PERCENT=50.000 PRIMER_LEFT_660_SELF_ANY=3.00 PRIMER_RIGHT_660_SELF_ANY=3.00 PRIMER_LEFT_660_SELF_END=0.00 PRIMER_RIGHT_660_SELF_END=2.00 PRIMER_LEFT_660_END_STABILITY=8.2000 PRIMER_RIGHT_660_END_STABILITY=8.9000 PRIMER_PAIR_660_COMPL_ANY=3.00 PRIMER_PAIR_660_COMPL_END=0.00 PRIMER_PAIR_660_PRODUCT_SIZE=232 PRIMER_PAIR_661_PENALTY=5.457774 PRIMER_LEFT_661_PENALTY=2.225062 PRIMER_RIGHT_661_PENALTY=3.232712 PRIMER_LEFT_661_SEQUENCE=ATGAATGTCCAGAATGGGAA PRIMER_RIGHT_661_SEQUENCE=AAGGGCATCACTTACAAACTCA PRIMER_LEFT_661=332,20 PRIMER_RIGHT_661=580,22 PRIMER_LEFT_661_TM=57.775 PRIMER_RIGHT_661_TM=58.767 PRIMER_LEFT_661_GC_PERCENT=40.000 PRIMER_RIGHT_661_GC_PERCENT=40.909 PRIMER_LEFT_661_SELF_ANY=3.00 PRIMER_RIGHT_661_SELF_ANY=4.00 PRIMER_LEFT_661_SELF_END=1.00 PRIMER_RIGHT_661_SELF_END=1.00 PRIMER_LEFT_661_END_STABILITY=9.7000 PRIMER_RIGHT_661_END_STABILITY=6.4000 PRIMER_PAIR_661_COMPL_ANY=4.00 PRIMER_PAIR_661_COMPL_END=0.00 PRIMER_PAIR_661_PRODUCT_SIZE=249 PRIMER_PAIR_662_PENALTY=5.465799 PRIMER_LEFT_662_PENALTY=2.171836 PRIMER_RIGHT_662_PENALTY=3.293963 PRIMER_LEFT_662_SEQUENCE=CACATGAATGTCCAGAATGGG PRIMER_RIGHT_662_SEQUENCE=CAAACTCACCAACTAAGCAGC PRIMER_LEFT_662=329,21 PRIMER_RIGHT_662=566,21 PRIMER_LEFT_662_TM=61.172 PRIMER_RIGHT_662_TM=57.706 PRIMER_LEFT_662_GC_PERCENT=47.619 PRIMER_RIGHT_662_GC_PERCENT=47.619 PRIMER_LEFT_662_SELF_ANY=6.00 PRIMER_RIGHT_662_SELF_ANY=3.00 PRIMER_LEFT_662_SELF_END=1.00 PRIMER_RIGHT_662_SELF_END=3.00 PRIMER_LEFT_662_END_STABILITY=9.6000 PRIMER_RIGHT_662_END_STABILITY=9.7000 PRIMER_PAIR_662_COMPL_ANY=3.00 PRIMER_PAIR_662_COMPL_END=1.00 PRIMER_PAIR_662_PRODUCT_SIZE=238 PRIMER_PAIR_663_PENALTY=5.624538 PRIMER_LEFT_663_PENALTY=2.303792 PRIMER_RIGHT_663_PENALTY=3.320745 PRIMER_LEFT_663_SEQUENCE=ATGAATGTCCAGAATGGGAAGT PRIMER_RIGHT_663_SEQUENCE=CTCACCAACTAAGCAGCGGTAG PRIMER_LEFT_663=332,22 PRIMER_RIGHT_663=562,22 PRIMER_LEFT_663_TM=59.696 PRIMER_RIGHT_663_TM=61.321 PRIMER_LEFT_663_GC_PERCENT=40.909 PRIMER_RIGHT_663_GC_PERCENT=54.545 PRIMER_LEFT_663_SELF_ANY=3.00 PRIMER_RIGHT_663_SELF_ANY=3.00 PRIMER_LEFT_663_SELF_END=1.00 PRIMER_RIGHT_663_SELF_END=3.00 PRIMER_LEFT_663_END_STABILITY=6.4000 PRIMER_RIGHT_663_END_STABILITY=6.9000 PRIMER_PAIR_663_COMPL_ANY=3.00 PRIMER_PAIR_663_COMPL_END=0.00 PRIMER_PAIR_663_PRODUCT_SIZE=231 PRIMER_PAIR_664_PENALTY=5.762404 PRIMER_LEFT_664_PENALTY=4.067900 PRIMER_RIGHT_664_PENALTY=1.694503 PRIMER_LEFT_664_SEQUENCE=TTCCTGACAAGTGCAAATTCTTA PRIMER_RIGHT_664_SEQUENCE=CCACCAGACATCCGAGTCA PRIMER_LEFT_664=585,23 PRIMER_RIGHT_664=829,19 PRIMER_LEFT_664_TM=58.932 PRIMER_RIGHT_664_TM=60.695 PRIMER_LEFT_664_GC_PERCENT=34.783 PRIMER_RIGHT_664_GC_PERCENT=57.895 PRIMER_LEFT_664_SELF_ANY=4.00 PRIMER_RIGHT_664_SELF_ANY=5.00 PRIMER_LEFT_664_SELF_END=2.00 PRIMER_RIGHT_664_SELF_END=3.00 PRIMER_LEFT_664_END_STABILITY=6.0000 PRIMER_RIGHT_664_END_STABILITY=6.4000 PRIMER_PAIR_664_COMPL_ANY=4.00 PRIMER_PAIR_664_COMPL_END=1.00 PRIMER_PAIR_664_PRODUCT_SIZE=245 PRIMER_PAIR_665_PENALTY=5.858935 PRIMER_LEFT_665_PENALTY=4.134169 PRIMER_RIGHT_665_PENALTY=1.724766 PRIMER_LEFT_665_SEQUENCE=GTTTGTAAGTGATGCCCTTCTC PRIMER_RIGHT_665_SEQUENCE=ACTTTCTTCAGCCAGTGGGC PRIMER_LEFT_665=562,22 PRIMER_RIGHT_665=778,20 PRIMER_LEFT_665_TM=57.866 PRIMER_RIGHT_665_TM=61.725 PRIMER_LEFT_665_GC_PERCENT=45.455 PRIMER_RIGHT_665_GC_PERCENT=55.000 PRIMER_LEFT_665_SELF_ANY=4.00 PRIMER_RIGHT_665_SELF_ANY=5.00 PRIMER_LEFT_665_SELF_END=2.00 PRIMER_RIGHT_665_SELF_END=3.00 PRIMER_LEFT_665_END_STABILITY=6.7000 PRIMER_RIGHT_665_END_STABILITY=11.2000 PRIMER_PAIR_665_COMPL_ANY=4.00 PRIMER_PAIR_665_COMPL_END=2.00 PRIMER_PAIR_665_PRODUCT_SIZE=217 PRIMER_PAIR_666_PENALTY=5.947785 PRIMER_LEFT_666_PENALTY=4.134169 PRIMER_RIGHT_666_PENALTY=1.813616 PRIMER_LEFT_666_SEQUENCE=GAGTTTGTAAGTGATGCCCTTC PRIMER_RIGHT_666_SEQUENCE=CAGCAGAATCCACATTGTCA PRIMER_LEFT_666=560,22 PRIMER_RIGHT_666=797,20 PRIMER_LEFT_666_TM=57.866 PRIMER_RIGHT_666_TM=58.186 PRIMER_LEFT_666_GC_PERCENT=45.455 PRIMER_RIGHT_666_GC_PERCENT=45.000 PRIMER_LEFT_666_SELF_ANY=4.00 PRIMER_RIGHT_666_SELF_ANY=5.00 PRIMER_LEFT_666_SELF_END=2.00 PRIMER_RIGHT_666_SELF_END=3.00 PRIMER_LEFT_666_END_STABILITY=8.2000 PRIMER_RIGHT_666_END_STABILITY=6.7000 PRIMER_PAIR_666_COMPL_ANY=3.00 PRIMER_PAIR_666_COMPL_END=1.00 PRIMER_PAIR_666_PRODUCT_SIZE=238 PRIMER_PAIR_667_PENALTY=5.950952 PRIMER_LEFT_667_PENALTY=4.895143 PRIMER_RIGHT_667_PENALTY=1.055809 PRIMER_LEFT_667_SEQUENCE=GGATCCCACTCGCACAGC PRIMER_RIGHT_667_SEQUENCE=CAGAACATGGCAATCTGGG PRIMER_LEFT_667=152,18 PRIMER_RIGHT_667=312,19 PRIMER_LEFT_667_TM=62.895 PRIMER_RIGHT_667_TM=60.056 PRIMER_LEFT_667_GC_PERCENT=66.667 PRIMER_RIGHT_667_GC_PERCENT=52.632 PRIMER_LEFT_667_SELF_ANY=6.00 PRIMER_RIGHT_667_SELF_ANY=4.00 PRIMER_LEFT_667_SELF_END=2.00 PRIMER_RIGHT_667_SELF_END=0.00 PRIMER_LEFT_667_END_STABILITY=7.9000 PRIMER_RIGHT_667_END_STABILITY=9.7000 PRIMER_PAIR_667_COMPL_ANY=4.00 PRIMER_PAIR_667_COMPL_END=2.00 PRIMER_PAIR_667_PRODUCT_SIZE=161 PRIMER_PAIR_668_PENALTY=5.988098 PRIMER_LEFT_668_PENALTY=2.578834 PRIMER_RIGHT_668_PENALTY=3.409264 PRIMER_LEFT_668_SEQUENCE=AATGTCCAGAATGGGAAGTG PRIMER_RIGHT_668_SEQUENCE=ACTAAGCAGCGGTAGGGAA PRIMER_LEFT_668=335,20 PRIMER_RIGHT_668=555,19 PRIMER_LEFT_668_TM=57.421 PRIMER_RIGHT_668_TM=57.591 PRIMER_LEFT_668_GC_PERCENT=45.000 PRIMER_RIGHT_668_GC_PERCENT=52.632 PRIMER_LEFT_668_SELF_ANY=3.00 PRIMER_RIGHT_668_SELF_ANY=3.00 PRIMER_LEFT_668_SELF_END=0.00 PRIMER_RIGHT_668_SELF_END=0.00 PRIMER_LEFT_668_END_STABILITY=6.7000 PRIMER_RIGHT_668_END_STABILITY=9.7000 PRIMER_PAIR_668_COMPL_ANY=3.00 PRIMER_PAIR_668_COMPL_END=0.00 PRIMER_PAIR_668_PRODUCT_SIZE=221 PRIMER_PAIR_669_PENALTY=6.001378 PRIMER_LEFT_669_PENALTY=4.361392 PRIMER_RIGHT_669_PENALTY=1.639986 PRIMER_LEFT_669_SEQUENCE=TCCGTCAGTTTCCTCGGC PRIMER_RIGHT_669_SEQUENCE=AGCAGGCCAGCATTACCAT PRIMER_LEFT_669=47,18 PRIMER_RIGHT_669=285,19 PRIMER_LEFT_669_TM=62.361 PRIMER_RIGHT_669_TM=60.640 PRIMER_LEFT_669_GC_PERCENT=61.111 PRIMER_RIGHT_669_GC_PERCENT=52.632 PRIMER_LEFT_669_SELF_ANY=3.00 PRIMER_RIGHT_669_SELF_ANY=6.00 PRIMER_LEFT_669_SELF_END=2.00 PRIMER_RIGHT_669_SELF_END=2.00 PRIMER_LEFT_669_END_STABILITY=11.4000 PRIMER_RIGHT_669_END_STABILITY=7.8000 PRIMER_PAIR_669_COMPL_ANY=3.00 PRIMER_PAIR_669_COMPL_END=0.00 PRIMER_PAIR_669_PRODUCT_SIZE=239 PRIMER_PAIR_670_PENALTY=6.005950 PRIMER_LEFT_670_PENALTY=4.159919 PRIMER_RIGHT_670_PENALTY=1.846031 PRIMER_LEFT_670_SEQUENCE=AGTTTGTAAGTGATGCCCTTCTC PRIMER_RIGHT_670_SEQUENCE=CAGCAGAATCCACATTGTCAC PRIMER_LEFT_670=561,23 PRIMER_RIGHT_670=797,21 PRIMER_LEFT_670_TM=58.840 PRIMER_RIGHT_670_TM=59.154 PRIMER_LEFT_670_GC_PERCENT=43.478 PRIMER_RIGHT_670_GC_PERCENT=47.619 PRIMER_LEFT_670_SELF_ANY=4.00 PRIMER_RIGHT_670_SELF_ANY=5.00 PRIMER_LEFT_670_SELF_END=2.00 PRIMER_RIGHT_670_SELF_END=3.00 PRIMER_LEFT_670_END_STABILITY=6.7000 PRIMER_RIGHT_670_END_STABILITY=6.1000 PRIMER_PAIR_670_COMPL_ANY=4.00 PRIMER_PAIR_670_COMPL_END=0.00 PRIMER_PAIR_670_PRODUCT_SIZE=237 PRIMER_PAIR_671_PENALTY=6.036992 PRIMER_LEFT_671_PENALTY=4.361392 PRIMER_RIGHT_671_PENALTY=1.675600 PRIMER_LEFT_671_SEQUENCE=TCGGGCTCCGTCAGTTTC PRIMER_RIGHT_671_SEQUENCE=CCATCAGTGGGTACCTCCA PRIMER_LEFT_671=41,18 PRIMER_RIGHT_671=270,19 PRIMER_LEFT_671_TM=62.361 PRIMER_RIGHT_671_TM=59.324 PRIMER_LEFT_671_GC_PERCENT=61.111 PRIMER_RIGHT_671_GC_PERCENT=57.895 PRIMER_LEFT_671_SELF_ANY=4.00 PRIMER_RIGHT_671_SELF_ANY=6.00 PRIMER_LEFT_671_SELF_END=0.00 PRIMER_RIGHT_671_SELF_END=2.00 PRIMER_LEFT_671_END_STABILITY=6.7000 PRIMER_RIGHT_671_END_STABILITY=8.2000 PRIMER_PAIR_671_COMPL_ANY=2.00 PRIMER_PAIR_671_COMPL_END=0.00 PRIMER_PAIR_671_PRODUCT_SIZE=230 PRIMER_PAIR_672_PENALTY=6.088629 PRIMER_LEFT_672_PENALTY=4.159919 PRIMER_RIGHT_672_PENALTY=1.928711 PRIMER_LEFT_672_SEQUENCE=GAGTTTGTAAGTGATGCCCTTCT PRIMER_RIGHT_672_SEQUENCE=CTTTCTTCAGCCAGTGGGC PRIMER_LEFT_672=560,23 PRIMER_RIGHT_672=777,19 PRIMER_LEFT_672_TM=58.840 PRIMER_RIGHT_672_TM=60.929 PRIMER_LEFT_672_GC_PERCENT=43.478 PRIMER_RIGHT_672_GC_PERCENT=57.895 PRIMER_LEFT_672_SELF_ANY=4.00 PRIMER_RIGHT_672_SELF_ANY=5.00 PRIMER_LEFT_672_SELF_END=0.00 PRIMER_RIGHT_672_SELF_END=3.00 PRIMER_LEFT_672_END_STABILITY=6.7000 PRIMER_RIGHT_672_END_STABILITY=11.2000 PRIMER_PAIR_672_COMPL_ANY=4.00 PRIMER_PAIR_672_COMPL_END=3.00 PRIMER_PAIR_672_PRODUCT_SIZE=218 PRIMER_PAIR_673_PENALTY=6.135491 PRIMER_LEFT_673_PENALTY=2.726227 PRIMER_RIGHT_673_PENALTY=3.409264 PRIMER_LEFT_673_SEQUENCE=CACATGAATGTCCAGAATGG PRIMER_RIGHT_673_SEQUENCE=AACTAAGCAGCGGTAGGGA PRIMER_LEFT_673=329,20 PRIMER_RIGHT_673=556,19 PRIMER_LEFT_673_TM=57.274 PRIMER_RIGHT_673_TM=57.591 PRIMER_LEFT_673_GC_PERCENT=45.000 PRIMER_RIGHT_673_GC_PERCENT=52.632 PRIMER_LEFT_673_SELF_ANY=6.00 PRIMER_RIGHT_673_SELF_ANY=3.00 PRIMER_LEFT_673_SELF_END=3.00 PRIMER_RIGHT_673_SELF_END=0.00 PRIMER_LEFT_673_END_STABILITY=8.4000 PRIMER_RIGHT_673_END_STABILITY=9.4000 PRIMER_PAIR_673_COMPL_ANY=3.00 PRIMER_PAIR_673_COMPL_END=0.00 PRIMER_PAIR_673_PRODUCT_SIZE=228 PRIMER_PAIR_674_PENALTY=6.135493 PRIMER_LEFT_674_PENALTY=4.291945 PRIMER_RIGHT_674_PENALTY=1.843548 PRIMER_LEFT_674_SEQUENCE=TTCCTGACAAGTGCAAATTCTTAC PRIMER_RIGHT_674_SEQUENCE=CAGACATCCGAGTCATCCTC PRIMER_LEFT_674=585,24 PRIMER_RIGHT_674=825,20 PRIMER_LEFT_674_TM=59.708 PRIMER_RIGHT_674_TM=58.156 PRIMER_LEFT_674_GC_PERCENT=37.500 PRIMER_RIGHT_674_GC_PERCENT=55.000 PRIMER_LEFT_674_SELF_ANY=4.00 PRIMER_RIGHT_674_SELF_ANY=5.00 PRIMER_LEFT_674_SELF_END=2.00 PRIMER_RIGHT_674_SELF_END=2.00 PRIMER_LEFT_674_END_STABILITY=5.7000 PRIMER_RIGHT_674_END_STABILITY=7.9000 PRIMER_PAIR_674_COMPL_ANY=4.00 PRIMER_PAIR_674_COMPL_END=0.00 PRIMER_PAIR_674_PRODUCT_SIZE=241 PRIMER_PAIR_675_PENALTY=6.154322 PRIMER_LEFT_675_PENALTY=2.740545 PRIMER_RIGHT_675_PENALTY=3.413777 PRIMER_LEFT_675_SEQUENCE=GATCCATCAGGGACCAAAA PRIMER_RIGHT_675_SEQUENCE=ACAAACTCACCAACTAAGCAGC PRIMER_LEFT_675=362,19 PRIMER_RIGHT_675=567,22 PRIMER_LEFT_675_TM=58.259 PRIMER_RIGHT_675_TM=58.586 PRIMER_LEFT_675_GC_PERCENT=47.368 PRIMER_RIGHT_675_GC_PERCENT=45.455 PRIMER_LEFT_675_SELF_ANY=5.00 PRIMER_RIGHT_675_SELF_ANY=3.00 PRIMER_LEFT_675_SELF_END=0.00 PRIMER_RIGHT_675_SELF_END=3.00 PRIMER_LEFT_675_END_STABILITY=7.6000 PRIMER_RIGHT_675_END_STABILITY=9.7000 PRIMER_PAIR_675_COMPL_ANY=2.00 PRIMER_PAIR_675_COMPL_END=0.00 PRIMER_PAIR_675_PRODUCT_SIZE=206 PRIMER_PAIR_676_PENALTY=6.162464 PRIMER_LEFT_676_PENALTY=2.740545 PRIMER_RIGHT_676_PENALTY=3.421919 PRIMER_LEFT_676_SEQUENCE=GAATGGGAAGTGGGATTCA PRIMER_RIGHT_676_SEQUENCE=AAGGGCATCACTTACAAACTCAC PRIMER_LEFT_676=343,19 PRIMER_RIGHT_676=580,23 PRIMER_LEFT_676_TM=58.259 PRIMER_RIGHT_676_TM=59.578 PRIMER_LEFT_676_GC_PERCENT=47.368 PRIMER_RIGHT_676_GC_PERCENT=43.478 PRIMER_LEFT_676_SELF_ANY=4.00 PRIMER_RIGHT_676_SELF_ANY=4.00 PRIMER_LEFT_676_SELF_END=1.00 PRIMER_RIGHT_676_SELF_END=0.00 PRIMER_LEFT_676_END_STABILITY=6.9000 PRIMER_RIGHT_676_END_STABILITY=6.4000 PRIMER_PAIR_676_COMPL_ANY=5.00 PRIMER_PAIR_676_COMPL_END=2.00 PRIMER_PAIR_676_PRODUCT_SIZE=238 PRIMER_PAIR_677_PENALTY=6.219376 PRIMER_LEFT_677_PENALTY=4.291945 PRIMER_RIGHT_677_PENALTY=1.927431 PRIMER_LEFT_677_SEQUENCE=GTTCCTGACAAGTGCAAATTCTTA PRIMER_RIGHT_677_SEQUENCE=ACCAGACATCCGAGTCATCCT PRIMER_LEFT_677=584,24 PRIMER_RIGHT_677=827,21 PRIMER_LEFT_677_TM=59.708 PRIMER_RIGHT_677_TM=60.927 PRIMER_LEFT_677_GC_PERCENT=37.500 PRIMER_RIGHT_677_GC_PERCENT=52.381 PRIMER_LEFT_677_SELF_ANY=4.00 PRIMER_RIGHT_677_SELF_ANY=5.00 PRIMER_LEFT_677_SELF_END=2.00 PRIMER_RIGHT_677_SELF_END=1.00 PRIMER_LEFT_677_END_STABILITY=6.0000 PRIMER_RIGHT_677_END_STABILITY=7.8000 PRIMER_PAIR_677_COMPL_ANY=4.00 PRIMER_PAIR_677_COMPL_END=1.00 PRIMER_PAIR_677_PRODUCT_SIZE=244 PRIMER_PAIR_678_PENALTY=6.239278 PRIMER_LEFT_678_PENALTY=2.761803 PRIMER_RIGHT_678_PENALTY=3.477475 PRIMER_LEFT_678_SEQUENCE=TCAGGGACCAAAACCTGCAT PRIMER_RIGHT_678_SEQUENCE=CACCAACTAAGCAGCGGTA PRIMER_LEFT_678=368,20 PRIMER_RIGHT_678=560,19 PRIMER_LEFT_678_TM=62.762 PRIMER_RIGHT_678_TM=57.523 PRIMER_LEFT_678_GC_PERCENT=50.000 PRIMER_RIGHT_678_GC_PERCENT=52.632 PRIMER_LEFT_678_SELF_ANY=4.00 PRIMER_RIGHT_678_SELF_ANY=3.00 PRIMER_LEFT_678_SELF_END=2.00 PRIMER_RIGHT_678_SELF_END=2.00 PRIMER_LEFT_678_END_STABILITY=8.4000 PRIMER_RIGHT_678_END_STABILITY=8.9000 PRIMER_PAIR_678_COMPL_ANY=4.00 PRIMER_PAIR_678_COMPL_END=2.00 PRIMER_PAIR_678_PRODUCT_SIZE=193 PRIMER_PAIR_679_PENALTY=6.248071 PRIMER_LEFT_679_PENALTY=2.761803 PRIMER_RIGHT_679_PENALTY=3.486268 PRIMER_LEFT_679_SEQUENCE=CATCAGGGACCAAAACCTGC PRIMER_RIGHT_679_SEQUENCE=TCACCAACTAAGCAGCGG PRIMER_LEFT_679=366,20 PRIMER_RIGHT_679=561,18 PRIMER_LEFT_679_TM=62.762 PRIMER_RIGHT_679_TM=58.514 PRIMER_LEFT_679_GC_PERCENT=55.000 PRIMER_RIGHT_679_GC_PERCENT=55.556 PRIMER_LEFT_679_SELF_ANY=4.00 PRIMER_RIGHT_679_SELF_ANY=3.00 PRIMER_LEFT_679_SELF_END=2.00 PRIMER_RIGHT_679_SELF_END=2.00 PRIMER_LEFT_679_END_STABILITY=9.7000 PRIMER_RIGHT_679_END_STABILITY=11.4000 PRIMER_PAIR_679_COMPL_ANY=4.00 PRIMER_PAIR_679_COMPL_END=2.00 PRIMER_PAIR_679_PRODUCT_SIZE=196 PRIMER_PAIR_680_PENALTY=6.251530 PRIMER_LEFT_680_PENALTY=4.284304 PRIMER_RIGHT_680_PENALTY=1.967225 PRIMER_LEFT_680_SEQUENCE=GATGCCCTTCTCGTTCCT PRIMER_RIGHT_680_SEQUENCE=CATCCGAGTCATCCTCCTCC PRIMER_LEFT_680=572,18 PRIMER_RIGHT_680=821,20 PRIMER_LEFT_680_TM=57.716 PRIMER_RIGHT_680_TM=61.967 PRIMER_LEFT_680_GC_PERCENT=55.556 PRIMER_RIGHT_680_GC_PERCENT=60.000 PRIMER_LEFT_680_SELF_ANY=2.00 PRIMER_RIGHT_680_SELF_ANY=3.00 PRIMER_LEFT_680_SELF_END=0.00 PRIMER_RIGHT_680_SELF_END=0.00 PRIMER_LEFT_680_END_STABILITY=8.2000 PRIMER_RIGHT_680_END_STABILITY=9.4000 PRIMER_PAIR_680_COMPL_ANY=4.00 PRIMER_PAIR_680_COMPL_END=0.00 PRIMER_PAIR_680_PRODUCT_SIZE=250 PRIMER_PAIR_681_PENALTY=6.327733 PRIMER_LEFT_681_PENALTY=2.831979 PRIMER_RIGHT_681_PENALTY=3.495754 PRIMER_LEFT_681_SEQUENCE=ATCCATCAGGGACCAAAACCT PRIMER_RIGHT_681_SEQUENCE=CGAGAAGGGCATCACTTACAAA PRIMER_LEFT_681=363,21 PRIMER_RIGHT_681=584,22 PRIMER_LEFT_681_TM=61.832 PRIMER_RIGHT_681_TM=61.496 PRIMER_LEFT_681_GC_PERCENT=47.619 PRIMER_RIGHT_681_GC_PERCENT=45.455 PRIMER_LEFT_681_SELF_ANY=5.00 PRIMER_RIGHT_681_SELF_ANY=4.00 PRIMER_LEFT_681_SELF_END=0.00 PRIMER_RIGHT_681_SELF_END=1.00 PRIMER_LEFT_681_END_STABILITY=7.9000 PRIMER_RIGHT_681_END_STABILITY=7.0000 PRIMER_PAIR_681_COMPL_ANY=3.00 PRIMER_PAIR_681_COMPL_END=1.00 PRIMER_PAIR_681_PRODUCT_SIZE=222 PRIMER_PAIR_682_PENALTY=6.436450 PRIMER_LEFT_682_PENALTY=4.426754 PRIMER_RIGHT_682_PENALTY=2.009696 PRIMER_LEFT_682_SEQUENCE=TCCTGACAAGTGCAAATTCTTA PRIMER_RIGHT_682_SEQUENCE=ATCCGAGTCATCCTCCTCC PRIMER_LEFT_682=586,22 PRIMER_RIGHT_682=820,19 PRIMER_LEFT_682_TM=57.573 PRIMER_RIGHT_682_TM=58.990 PRIMER_LEFT_682_GC_PERCENT=36.364 PRIMER_RIGHT_682_GC_PERCENT=57.895 PRIMER_LEFT_682_SELF_ANY=4.00 PRIMER_RIGHT_682_SELF_ANY=3.00 PRIMER_LEFT_682_SELF_END=2.00 PRIMER_RIGHT_682_SELF_END=0.00 PRIMER_LEFT_682_END_STABILITY=6.0000 PRIMER_RIGHT_682_END_STABILITY=9.4000 PRIMER_PAIR_682_COMPL_ANY=4.00 PRIMER_PAIR_682_COMPL_END=0.00 PRIMER_PAIR_682_PRODUCT_SIZE=235 PRIMER_PAIR_683_PENALTY=6.579028 PRIMER_LEFT_683_PENALTY=4.560670 PRIMER_RIGHT_683_PENALTY=2.018358 PRIMER_LEFT_683_SEQUENCE=TCCTGACAAGTGCAAATTCTTAC PRIMER_RIGHT_683_SEQUENCE=GACATCCGAGTCATCCTCCTC PRIMER_LEFT_683=586,23 PRIMER_RIGHT_683=823,21 PRIMER_LEFT_683_TM=58.439 PRIMER_RIGHT_683_TM=61.018 PRIMER_LEFT_683_GC_PERCENT=39.130 PRIMER_RIGHT_683_GC_PERCENT=57.143 PRIMER_LEFT_683_SELF_ANY=4.00 PRIMER_RIGHT_683_SELF_ANY=5.00 PRIMER_LEFT_683_SELF_END=2.00 PRIMER_RIGHT_683_SELF_END=0.00 PRIMER_LEFT_683_END_STABILITY=5.7000 PRIMER_RIGHT_683_END_STABILITY=7.9000 PRIMER_PAIR_683_COMPL_ANY=4.00 PRIMER_PAIR_683_COMPL_END=0.00 PRIMER_PAIR_683_PRODUCT_SIZE=238 PRIMER_PAIR_684_PENALTY=6.604277 PRIMER_LEFT_684_PENALTY=4.521959 PRIMER_RIGHT_684_PENALTY=2.082318 PRIMER_LEFT_684_SEQUENCE=GAGTTTGTAAGTGATGCCCTTCTC PRIMER_RIGHT_684_SEQUENCE=TTTCTTCAGCCAGTGGGC PRIMER_LEFT_684=560,24 PRIMER_RIGHT_684=776,18 PRIMER_LEFT_684_TM=60.522 PRIMER_RIGHT_684_TM=59.918 PRIMER_LEFT_684_GC_PERCENT=45.833 PRIMER_RIGHT_684_GC_PERCENT=55.556 PRIMER_LEFT_684_SELF_ANY=4.00 PRIMER_RIGHT_684_SELF_ANY=5.00 PRIMER_LEFT_684_SELF_END=2.00 PRIMER_RIGHT_684_SELF_END=3.00 PRIMER_LEFT_684_END_STABILITY=6.7000 PRIMER_RIGHT_684_END_STABILITY=11.2000 PRIMER_PAIR_684_COMPL_ANY=4.00 PRIMER_PAIR_684_COMPL_END=2.00 PRIMER_PAIR_684_PRODUCT_SIZE=217 PRIMER_PAIR_685_PENALTY=6.605800 PRIMER_LEFT_685_PENALTY=3.077974 PRIMER_RIGHT_685_PENALTY=3.527826 PRIMER_LEFT_685_SEQUENCE=GCCAAGAAGTCTACCCTGAACT PRIMER_RIGHT_685_SEQUENCE=GAAGGGCATCACTTACAAACTCA PRIMER_LEFT_685=417,22 PRIMER_RIGHT_685=581,23 PRIMER_LEFT_685_TM=58.922 PRIMER_RIGHT_685_TM=60.528 PRIMER_LEFT_685_GC_PERCENT=50.000 PRIMER_RIGHT_685_GC_PERCENT=43.478 PRIMER_LEFT_685_SELF_ANY=4.00 PRIMER_RIGHT_685_SELF_ANY=4.00 PRIMER_LEFT_685_SELF_END=1.00 PRIMER_RIGHT_685_SELF_END=1.00 PRIMER_LEFT_685_END_STABILITY=6.4000 PRIMER_RIGHT_685_END_STABILITY=6.4000 PRIMER_PAIR_685_COMPL_ANY=5.00 PRIMER_PAIR_685_COMPL_END=2.00 PRIMER_PAIR_685_PRODUCT_SIZE=165 PRIMER_PAIR_686_PENALTY=6.675183 PRIMER_LEFT_686_PENALTY=4.414025 PRIMER_RIGHT_686_PENALTY=2.261158 PRIMER_LEFT_686_SEQUENCE=TTTCCTCGGCAGCGGTAG PRIMER_RIGHT_686_SEQUENCE=CAGTGGGTACCTCCAGCG PRIMER_LEFT_686=55,18 PRIMER_RIGHT_686=266,18 PRIMER_LEFT_686_TM=62.414 PRIMER_RIGHT_686_TM=60.261 PRIMER_LEFT_686_GC_PERCENT=61.111 PRIMER_RIGHT_686_GC_PERCENT=66.667 PRIMER_LEFT_686_SELF_ANY=4.00 PRIMER_RIGHT_686_SELF_ANY=6.00 PRIMER_LEFT_686_SELF_END=3.00 PRIMER_RIGHT_686_SELF_END=2.00 PRIMER_LEFT_686_END_STABILITY=6.9000 PRIMER_RIGHT_686_END_STABILITY=10.2000 PRIMER_PAIR_686_COMPL_ANY=4.00 PRIMER_PAIR_686_COMPL_END=2.00 PRIMER_PAIR_686_PRODUCT_SIZE=212 PRIMER_PAIR_687_PENALTY=6.718648 PRIMER_LEFT_687_PENALTY=4.632309 PRIMER_RIGHT_687_PENALTY=2.086339 PRIMER_LEFT_687_SEQUENCE=GGTGAGTTTGTAAGTGATGCCCT PRIMER_RIGHT_687_SEQUENCE=CATTGTCACTTTCTTCAGCCAG PRIMER_LEFT_687=557,23 PRIMER_RIGHT_687=785,22 PRIMER_LEFT_687_TM=61.632 PRIMER_RIGHT_687_TM=59.914 PRIMER_LEFT_687_GC_PERCENT=47.826 PRIMER_RIGHT_687_GC_PERCENT=45.455 PRIMER_LEFT_687_SELF_ANY=2.00 PRIMER_RIGHT_687_SELF_ANY=3.00 PRIMER_LEFT_687_SELF_END=2.00 PRIMER_RIGHT_687_SELF_END=2.00 PRIMER_LEFT_687_END_STABILITY=10.9000 PRIMER_RIGHT_687_END_STABILITY=9.7000 PRIMER_PAIR_687_COMPL_ANY=6.00 PRIMER_PAIR_687_COMPL_END=2.00 PRIMER_PAIR_687_PRODUCT_SIZE=229 PRIMER_PAIR_688_PENALTY=6.773585 PRIMER_LEFT_688_PENALTY=4.685119 PRIMER_RIGHT_688_PENALTY=2.088466 PRIMER_LEFT_688_SEQUENCE=TAAGTGATGCCCTTCTCGTTCC PRIMER_RIGHT_688_SEQUENCE=CATTGTCACTTTCTTCAGCCA PRIMER_LEFT_688=567,22 PRIMER_RIGHT_688=785,21 PRIMER_LEFT_688_TM=62.685 PRIMER_RIGHT_688_TM=58.912 PRIMER_LEFT_688_GC_PERCENT=50.000 PRIMER_RIGHT_688_GC_PERCENT=42.857 PRIMER_LEFT_688_SELF_ANY=4.00 PRIMER_RIGHT_688_SELF_ANY=3.00 PRIMER_LEFT_688_SELF_END=0.00 PRIMER_RIGHT_688_SELF_END=0.00 PRIMER_LEFT_688_END_STABILITY=7.9000 PRIMER_RIGHT_688_END_STABILITY=9.7000 PRIMER_PAIR_688_COMPL_ANY=6.00 PRIMER_PAIR_688_COMPL_END=0.00 PRIMER_PAIR_688_PRODUCT_SIZE=219 PRIMER_PAIR_689_PENALTY=6.808167 PRIMER_LEFT_689_PENALTY=3.152169 PRIMER_RIGHT_689_PENALTY=3.655999 PRIMER_LEFT_689_SEQUENCE=CAGATCCATCAGGGACCAAAA PRIMER_RIGHT_689_SEQUENCE=AGGGCATCACTTACAAACTCA PRIMER_LEFT_689=360,21 PRIMER_RIGHT_689=579,21 PRIMER_LEFT_689_TM=62.152 PRIMER_RIGHT_689_TM=57.344 PRIMER_LEFT_689_GC_PERCENT=47.619 PRIMER_RIGHT_689_GC_PERCENT=42.857 PRIMER_LEFT_689_SELF_ANY=5.00 PRIMER_RIGHT_689_SELF_ANY=2.00 PRIMER_LEFT_689_SELF_END=0.00 PRIMER_RIGHT_689_SELF_END=1.00 PRIMER_LEFT_689_END_STABILITY=7.6000 PRIMER_RIGHT_689_END_STABILITY=6.4000 PRIMER_PAIR_689_COMPL_ANY=3.00 PRIMER_PAIR_689_COMPL_END=0.00 PRIMER_PAIR_689_PRODUCT_SIZE=220 PRIMER_PAIR_690_PENALTY=6.863341 PRIMER_LEFT_690_PENALTY=3.152169 PRIMER_RIGHT_690_PENALTY=3.711172 PRIMER_LEFT_690_SEQUENCE=CAGAATGGGAAGTGGGATTCA PRIMER_RIGHT_690_SEQUENCE=ACCAACTAAGCAGCGGTAGGG PRIMER_LEFT_690=341,21 PRIMER_RIGHT_690=559,21 PRIMER_LEFT_690_TM=62.152 PRIMER_RIGHT_690_TM=62.711 PRIMER_LEFT_690_GC_PERCENT=47.619 PRIMER_RIGHT_690_GC_PERCENT=57.143 PRIMER_LEFT_690_SELF_ANY=4.00 PRIMER_RIGHT_690_SELF_ANY=3.00 PRIMER_LEFT_690_SELF_END=1.00 PRIMER_RIGHT_690_SELF_END=0.00 PRIMER_LEFT_690_END_STABILITY=6.9000 PRIMER_RIGHT_690_END_STABILITY=8.7000 PRIMER_PAIR_690_COMPL_ANY=4.00 PRIMER_PAIR_690_COMPL_END=0.00 PRIMER_PAIR_690_PRODUCT_SIZE=219 PRIMER_PAIR_691_PENALTY=6.912901 PRIMER_LEFT_691_PENALTY=3.165686 PRIMER_RIGHT_691_PENALTY=3.747215 PRIMER_LEFT_691_SEQUENCE=AGATCCATCAGGGACCAAA PRIMER_RIGHT_691_SEQUENCE=AGGGCATCACTTACAAACTCAC PRIMER_LEFT_691=361,19 PRIMER_RIGHT_691=579,22 PRIMER_LEFT_691_TM=57.834 PRIMER_RIGHT_691_TM=58.253 PRIMER_LEFT_691_GC_PERCENT=47.368 PRIMER_RIGHT_691_GC_PERCENT=45.455 PRIMER_LEFT_691_SELF_ANY=5.00 PRIMER_RIGHT_691_SELF_ANY=2.00 PRIMER_LEFT_691_SELF_END=0.00 PRIMER_RIGHT_691_SELF_END=0.00 PRIMER_LEFT_691_END_STABILITY=8.8000 PRIMER_RIGHT_691_END_STABILITY=6.4000 PRIMER_PAIR_691_COMPL_ANY=3.00 PRIMER_PAIR_691_COMPL_END=0.00 PRIMER_PAIR_691_PRODUCT_SIZE=219 PRIMER_PAIR_692_PENALTY=6.937130 PRIMER_LEFT_692_PENALTY=4.837235 PRIMER_RIGHT_692_PENALTY=2.099895 PRIMER_LEFT_692_SEQUENCE=CCCTTCTCGTTCCTGACA PRIMER_RIGHT_692_SEQUENCE=CCTCCGCATCAGCAGAAT PRIMER_LEFT_692=576,18 PRIMER_RIGHT_692=806,18 PRIMER_LEFT_692_TM=57.163 PRIMER_RIGHT_692_TM=59.900 PRIMER_LEFT_692_GC_PERCENT=55.556 PRIMER_RIGHT_692_GC_PERCENT=55.556 PRIMER_LEFT_692_SELF_ANY=3.00 PRIMER_RIGHT_692_SELF_ANY=4.00 PRIMER_LEFT_692_SELF_END=3.00 PRIMER_RIGHT_692_SELF_END=2.00 PRIMER_LEFT_692_END_STABILITY=6.7000 PRIMER_RIGHT_692_END_STABILITY=6.6000 PRIMER_PAIR_692_COMPL_ANY=4.00 PRIMER_PAIR_692_COMPL_END=1.00 PRIMER_PAIR_692_PRODUCT_SIZE=231 PRIMER_PAIR_693_PENALTY=6.956167 PRIMER_LEFT_693_PENALTY=3.165686 PRIMER_RIGHT_693_PENALTY=3.790481 PRIMER_LEFT_693_SEQUENCE=TGGGAAGTGGGATTCAGAT PRIMER_RIGHT_693_SEQUENCE=CAACTAAGCAGCGGTAGGGAAT PRIMER_LEFT_693=346,19 PRIMER_RIGHT_693=557,22 PRIMER_LEFT_693_TM=57.834 PRIMER_RIGHT_693_TM=61.790 PRIMER_LEFT_693_GC_PERCENT=47.368 PRIMER_RIGHT_693_GC_PERCENT=50.000 PRIMER_LEFT_693_SELF_ANY=3.00 PRIMER_RIGHT_693_SELF_ANY=3.00 PRIMER_LEFT_693_SELF_END=2.00 PRIMER_RIGHT_693_SELF_END=2.00 PRIMER_LEFT_693_END_STABILITY=6.6000 PRIMER_RIGHT_693_END_STABILITY=8.1000 PRIMER_PAIR_693_COMPL_ANY=4.00 PRIMER_PAIR_693_COMPL_END=2.00 PRIMER_PAIR_693_PRODUCT_SIZE=212 PRIMER_PAIR_694_PENALTY=6.979428 PRIMER_LEFT_694_PENALTY=3.165686 PRIMER_RIGHT_694_PENALTY=3.813742 PRIMER_LEFT_694_SEQUENCE=ATGGGAAGTGGGATTCAGA PRIMER_RIGHT_694_SEQUENCE=GGCATCACTTACAAACTCACC PRIMER_LEFT_694=345,19 PRIMER_RIGHT_694=577,21 PRIMER_LEFT_694_TM=57.834 PRIMER_RIGHT_694_TM=57.186 PRIMER_LEFT_694_GC_PERCENT=47.368 PRIMER_RIGHT_694_GC_PERCENT=47.619 PRIMER_LEFT_694_SELF_ANY=3.00 PRIMER_RIGHT_694_SELF_ANY=2.00 PRIMER_LEFT_694_SELF_END=3.00 PRIMER_RIGHT_694_SELF_END=0.00 PRIMER_LEFT_694_END_STABILITY=6.7000 PRIMER_RIGHT_694_END_STABILITY=7.9000 PRIMER_PAIR_694_COMPL_ANY=5.00 PRIMER_PAIR_694_COMPL_END=1.00 PRIMER_PAIR_694_PRODUCT_SIZE=233 PRIMER_PAIR_695_PENALTY=7.036440 PRIMER_LEFT_695_PENALTY=3.222698 PRIMER_RIGHT_695_PENALTY=3.813742 PRIMER_LEFT_695_SEQUENCE=GAAGTGGGATTCAGATCCATCA PRIMER_RIGHT_695_SEQUENCE=GGGCATCACTTACAAACTCAC PRIMER_LEFT_695=349,22 PRIMER_RIGHT_695=578,21 PRIMER_LEFT_695_TM=61.223 PRIMER_RIGHT_695_TM=57.186 PRIMER_LEFT_695_GC_PERCENT=45.455 PRIMER_RIGHT_695_GC_PERCENT=47.619 PRIMER_LEFT_695_SELF_ANY=5.00 PRIMER_RIGHT_695_SELF_ANY=2.00 PRIMER_LEFT_695_SELF_END=2.00 PRIMER_RIGHT_695_SELF_END=0.00 PRIMER_LEFT_695_END_STABILITY=6.9000 PRIMER_RIGHT_695_END_STABILITY=6.4000 PRIMER_PAIR_695_COMPL_ANY=5.00 PRIMER_PAIR_695_COMPL_END=0.00 PRIMER_PAIR_695_PRODUCT_SIZE=230 PRIMER_PAIR_696_PENALTY=7.047570 PRIMER_LEFT_696_PENALTY=5.028844 PRIMER_RIGHT_696_PENALTY=2.018726 PRIMER_LEFT_696_SEQUENCE=TCCTGACAAGTGCAAATTCTTACA PRIMER_RIGHT_696_SEQUENCE=CCAGACATCCGAGTCATCC PRIMER_LEFT_696=586,24 PRIMER_RIGHT_696=826,19 PRIMER_LEFT_696_TM=61.029 PRIMER_RIGHT_696_TM=58.981 PRIMER_LEFT_696_GC_PERCENT=37.500 PRIMER_RIGHT_696_GC_PERCENT=57.895 PRIMER_LEFT_696_SELF_ANY=4.00 PRIMER_RIGHT_696_SELF_ANY=5.00 PRIMER_LEFT_696_SELF_END=3.00 PRIMER_RIGHT_696_SELF_END=0.00 PRIMER_LEFT_696_END_STABILITY=6.0000 PRIMER_RIGHT_696_END_STABILITY=8.1000 PRIMER_PAIR_696_COMPL_ANY=4.00 PRIMER_PAIR_696_COMPL_END=0.00 PRIMER_PAIR_696_PRODUCT_SIZE=241 PRIMER_PAIR_697_PENALTY=7.102820 PRIMER_LEFT_697_PENALTY=3.228511 PRIMER_RIGHT_697_PENALTY=3.874309 PRIMER_LEFT_697_SEQUENCE=GGATTCAGATCCATCAGGG PRIMER_RIGHT_697_SEQUENCE=CAGCGGTAGGGAATCACA PRIMER_LEFT_697=355,19 PRIMER_RIGHT_697=549,18 PRIMER_LEFT_697_TM=57.771 PRIMER_RIGHT_697_TM=58.126 PRIMER_LEFT_697_GC_PERCENT=52.632 PRIMER_RIGHT_697_GC_PERCENT=55.556 PRIMER_LEFT_697_SELF_ANY=5.00 PRIMER_RIGHT_697_SELF_ANY=3.00 PRIMER_LEFT_697_SELF_END=3.00 PRIMER_RIGHT_697_SELF_END=1.00 PRIMER_LEFT_697_END_STABILITY=9.7000 PRIMER_RIGHT_697_END_STABILITY=6.7000 PRIMER_PAIR_697_COMPL_ANY=5.00 PRIMER_PAIR_697_COMPL_END=0.00 PRIMER_PAIR_697_PRODUCT_SIZE=195 PRIMER_PAIR_698_PENALTY=7.118286 PRIMER_LEFT_698_PENALTY=4.939568 PRIMER_RIGHT_698_PENALTY=2.178718 PRIMER_LEFT_698_SEQUENCE=AGTTTGTAAGTGATGCCCTTCT PRIMER_RIGHT_698_SEQUENCE=CAGCAGAATCCACATTGTCACT PRIMER_LEFT_698=561,22 PRIMER_RIGHT_698=797,22 PRIMER_LEFT_698_TM=57.060 PRIMER_RIGHT_698_TM=60.179 PRIMER_LEFT_698_GC_PERCENT=40.909 PRIMER_RIGHT_698_GC_PERCENT=45.455 PRIMER_LEFT_698_SELF_ANY=4.00 PRIMER_RIGHT_698_SELF_ANY=5.00 PRIMER_LEFT_698_SELF_END=0.00 PRIMER_RIGHT_698_SELF_END=3.00 PRIMER_LEFT_698_END_STABILITY=6.7000 PRIMER_RIGHT_698_END_STABILITY=6.4000 PRIMER_PAIR_698_COMPL_ANY=5.00 PRIMER_PAIR_698_COMPL_END=0.00 PRIMER_PAIR_698_PRODUCT_SIZE=237 PRIMER_PAIR_699_PENALTY=7.149466 PRIMER_LEFT_699_PENALTY=3.228511 PRIMER_RIGHT_699_PENALTY=3.920955 PRIMER_LEFT_699_SEQUENCE=GGGATTCAGATCCATCAGG PRIMER_RIGHT_699_SEQUENCE=CTAAGCAGCGGTAGGGAAT PRIMER_LEFT_699=354,19 PRIMER_RIGHT_699=554,19 PRIMER_LEFT_699_TM=57.771 PRIMER_RIGHT_699_TM=57.079 PRIMER_LEFT_699_GC_PERCENT=52.632 PRIMER_RIGHT_699_GC_PERCENT=52.632 PRIMER_LEFT_699_SELF_ANY=5.00 PRIMER_RIGHT_699_SELF_ANY=3.00 PRIMER_LEFT_699_SELF_END=2.00 PRIMER_RIGHT_699_SELF_END=2.00 PRIMER_LEFT_699_END_STABILITY=8.2000 PRIMER_RIGHT_699_END_STABILITY=8.1000 PRIMER_PAIR_699_COMPL_ANY=4.00 PRIMER_PAIR_699_COMPL_END=0.00 PRIMER_PAIR_699_PRODUCT_SIZE=201 PRIMER_PAIR_700_PENALTY=7.273914 PRIMER_LEFT_700_PENALTY=5.707913 PRIMER_RIGHT_700_PENALTY=1.566001 PRIMER_LEFT_700_SEQUENCE=AAACTCATCTTCACTGGCACACC PRIMER_RIGHT_700_SEQUENCE=TAGTCTGTGTCTGCTCCGCC PRIMER_LEFT_700=633,23 PRIMER_RIGHT_700=849,20 PRIMER_LEFT_700_TM=62.708 PRIMER_RIGHT_700_TM=61.566 PRIMER_LEFT_700_GC_PERCENT=47.826 PRIMER_RIGHT_700_GC_PERCENT=60.000 PRIMER_LEFT_700_SELF_ANY=3.00 PRIMER_RIGHT_700_SELF_ANY=3.00 PRIMER_LEFT_700_SELF_END=0.00 PRIMER_RIGHT_700_SELF_END=1.00 PRIMER_LEFT_700_END_STABILITY=7.6000 PRIMER_RIGHT_700_END_STABILITY=12.9000 PRIMER_PAIR_700_COMPL_ANY=4.00 PRIMER_PAIR_700_COMPL_END=2.00 PRIMER_PAIR_700_PRODUCT_SIZE=217 PRIMER_PAIR_701_PENALTY=7.380842 PRIMER_LEFT_701_PENALTY=5.190656 PRIMER_RIGHT_701_PENALTY=2.190186 PRIMER_LEFT_701_SEQUENCE=TTGTAAGTGATGCCCTTCTCGTT PRIMER_RIGHT_701_SEQUENCE=CGCATCAGCAGAATCCACAT PRIMER_LEFT_701=564,23 PRIMER_RIGHT_701=802,20 PRIMER_LEFT_701_TM=62.191 PRIMER_RIGHT_701_TM=62.190 PRIMER_LEFT_701_GC_PERCENT=43.478 PRIMER_RIGHT_701_GC_PERCENT=50.000 PRIMER_LEFT_701_SELF_ANY=4.00 PRIMER_RIGHT_701_SELF_ANY=4.00 PRIMER_LEFT_701_SELF_END=2.00 PRIMER_RIGHT_701_SELF_END=2.00 PRIMER_LEFT_701_END_STABILITY=8.4000 PRIMER_RIGHT_701_END_STABILITY=6.6000 PRIMER_PAIR_701_COMPL_ANY=6.00 PRIMER_PAIR_701_COMPL_END=1.00 PRIMER_PAIR_701_PRODUCT_SIZE=239 PRIMER_PAIR_702_PENALTY=7.385193 PRIMER_LEFT_702_PENALTY=5.253665 PRIMER_RIGHT_702_PENALTY=2.131528 PRIMER_LEFT_702_SEQUENCE=GTGAGTTTGTAAGTGATGCCCTTC PRIMER_RIGHT_702_SEQUENCE=CCAGTGGGCAACACACAA PRIMER_LEFT_702=558,24 PRIMER_RIGHT_702=767,18 PRIMER_LEFT_702_TM=61.254 PRIMER_RIGHT_702_TM=60.132 PRIMER_LEFT_702_GC_PERCENT=45.833 PRIMER_RIGHT_702_GC_PERCENT=55.556 PRIMER_LEFT_702_SELF_ANY=4.00 PRIMER_RIGHT_702_SELF_ANY=5.00 PRIMER_LEFT_702_SELF_END=2.00 PRIMER_RIGHT_702_SELF_END=0.00 PRIMER_LEFT_702_END_STABILITY=8.2000 PRIMER_RIGHT_702_END_STABILITY=7.0000 PRIMER_PAIR_702_COMPL_ANY=7.00 PRIMER_PAIR_702_COMPL_END=3.00 PRIMER_PAIR_702_PRODUCT_SIZE=210 PRIMER_PAIR_703_PENALTY=7.410540 PRIMER_LEFT_703_PENALTY=5.190656 PRIMER_RIGHT_703_PENALTY=2.219884 PRIMER_LEFT_703_SEQUENCE=TTTGTAAGTGATGCCCTTCTCGT PRIMER_RIGHT_703_SEQUENCE=ACATTGTCACTTTCTTCAGCCA PRIMER_LEFT_703=563,23 PRIMER_RIGHT_703=786,22 PRIMER_LEFT_703_TM=62.191 PRIMER_RIGHT_703_TM=59.780 PRIMER_LEFT_703_GC_PERCENT=43.478 PRIMER_RIGHT_703_GC_PERCENT=40.909 PRIMER_LEFT_703_SELF_ANY=4.00 PRIMER_RIGHT_703_SELF_ANY=5.00 PRIMER_LEFT_703_SELF_END=2.00 PRIMER_RIGHT_703_SELF_END=0.00 PRIMER_LEFT_703_END_STABILITY=8.1000 PRIMER_RIGHT_703_END_STABILITY=9.7000 PRIMER_PAIR_703_COMPL_ANY=6.00 PRIMER_PAIR_703_COMPL_END=1.00 PRIMER_PAIR_703_PRODUCT_SIZE=224 PRIMER_PAIR_704_PENALTY=7.410540 PRIMER_LEFT_704_PENALTY=5.190656 PRIMER_RIGHT_704_PENALTY=2.219884 PRIMER_LEFT_704_SEQUENCE=GTTTGTAAGTGATGCCCTTCTCG PRIMER_RIGHT_704_SEQUENCE=CACATTGTCACTTTCTTCAGCC PRIMER_LEFT_704=562,23 PRIMER_RIGHT_704=787,22 PRIMER_LEFT_704_TM=62.191 PRIMER_RIGHT_704_TM=59.780 PRIMER_LEFT_704_GC_PERCENT=47.826 PRIMER_RIGHT_704_GC_PERCENT=45.455 PRIMER_LEFT_704_SELF_ANY=4.00 PRIMER_RIGHT_704_SELF_ANY=5.00 PRIMER_LEFT_704_SELF_END=2.00 PRIMER_RIGHT_704_SELF_END=1.00 PRIMER_LEFT_704_END_STABILITY=8.4000 PRIMER_RIGHT_704_END_STABILITY=9.7000 PRIMER_PAIR_704_COMPL_ANY=6.00 PRIMER_PAIR_704_COMPL_END=1.00 PRIMER_PAIR_704_PRODUCT_SIZE=226 PRIMER_PAIR_705_PENALTY=7.421562 PRIMER_LEFT_705_PENALTY=3.309654 PRIMER_RIGHT_705_PENALTY=4.111908 PRIMER_LEFT_705_SEQUENCE=CCATCAGGGACCAAAACC PRIMER_RIGHT_705_SEQUENCE=TTACAAACTCACCAACTAAGCAGC PRIMER_LEFT_705=365,18 PRIMER_RIGHT_705=569,24 PRIMER_LEFT_705_TM=58.690 PRIMER_RIGHT_705_TM=59.888 PRIMER_LEFT_705_GC_PERCENT=55.556 PRIMER_RIGHT_705_GC_PERCENT=41.667 PRIMER_LEFT_705_SELF_ANY=3.00 PRIMER_RIGHT_705_SELF_ANY=3.00 PRIMER_LEFT_705_SELF_END=0.00 PRIMER_RIGHT_705_SELF_END=3.00 PRIMER_LEFT_705_END_STABILITY=8.2000 PRIMER_RIGHT_705_END_STABILITY=9.7000 PRIMER_PAIR_705_COMPL_ANY=2.00 PRIMER_PAIR_705_COMPL_END=0.00 PRIMER_PAIR_705_PRODUCT_SIZE=205 PRIMER_PAIR_706_PENALTY=7.515064 PRIMER_LEFT_706_PENALTY=3.380895 PRIMER_RIGHT_706_PENALTY=4.134169 PRIMER_LEFT_706_SEQUENCE=GTCCAGAATGGGAAGTGGGAT PRIMER_RIGHT_706_SEQUENCE=GAAGGGCATCACTTACAAACTC PRIMER_LEFT_706=338,21 PRIMER_RIGHT_706=581,22 PRIMER_LEFT_706_TM=62.381 PRIMER_RIGHT_706_TM=57.866 PRIMER_LEFT_706_GC_PERCENT=52.381 PRIMER_RIGHT_706_GC_PERCENT=45.455 PRIMER_LEFT_706_SELF_ANY=3.00 PRIMER_RIGHT_706_SELF_ANY=4.00 PRIMER_LEFT_706_SELF_END=2.00 PRIMER_RIGHT_706_SELF_END=0.00 PRIMER_LEFT_706_END_STABILITY=9.3000 PRIMER_RIGHT_706_END_STABILITY=6.4000 PRIMER_PAIR_706_COMPL_ANY=5.00 PRIMER_PAIR_706_COMPL_END=2.00 PRIMER_PAIR_706_PRODUCT_SIZE=244 PRIMER_PAIR_707_PENALTY=7.541935 PRIMER_LEFT_707_PENALTY=3.407766 PRIMER_RIGHT_707_PENALTY=4.134169 PRIMER_LEFT_707_SEQUENCE=GAATGGGAAGTGGGATTCAGAT PRIMER_RIGHT_707_SEQUENCE=GAGAAGGGCATCACTTACAAAC PRIMER_LEFT_707=343,22 PRIMER_RIGHT_707=583,22 PRIMER_LEFT_707_TM=61.408 PRIMER_RIGHT_707_TM=57.866 PRIMER_LEFT_707_GC_PERCENT=45.455 PRIMER_RIGHT_707_GC_PERCENT=45.455 PRIMER_LEFT_707_SELF_ANY=4.00 PRIMER_RIGHT_707_SELF_ANY=4.00 PRIMER_LEFT_707_SELF_END=2.00 PRIMER_RIGHT_707_SELF_END=0.00 PRIMER_LEFT_707_END_STABILITY=6.6000 PRIMER_RIGHT_707_END_STABILITY=7.0000 PRIMER_PAIR_707_COMPL_ANY=5.00 PRIMER_PAIR_707_COMPL_END=1.00 PRIMER_PAIR_707_PRODUCT_SIZE=241 PRIMER_PAIR_708_PENALTY=7.553264 PRIMER_LEFT_708_PENALTY=5.431239 PRIMER_RIGHT_708_PENALTY=2.122026 PRIMER_LEFT_708_SEQUENCE=GTTCCTGACAAGTGCAAATTCTTAC PRIMER_RIGHT_708_SEQUENCE=GAGTCATCCTCCTCCGCATC PRIMER_LEFT_708=584,25 PRIMER_RIGHT_708=816,20 PRIMER_LEFT_708_TM=60.431 PRIMER_RIGHT_708_TM=62.122 PRIMER_LEFT_708_GC_PERCENT=40.000 PRIMER_RIGHT_708_GC_PERCENT=60.000 PRIMER_LEFT_708_SELF_ANY=4.00 PRIMER_RIGHT_708_SELF_ANY=3.00 PRIMER_LEFT_708_SELF_END=2.00 PRIMER_RIGHT_708_SELF_END=0.00 PRIMER_LEFT_708_END_STABILITY=5.7000 PRIMER_RIGHT_708_END_STABILITY=8.1000 PRIMER_PAIR_708_COMPL_ANY=4.00 PRIMER_PAIR_708_COMPL_END=0.00 PRIMER_PAIR_708_PRODUCT_SIZE=233 PRIMER_PAIR_709_PENALTY=7.583554 PRIMER_LEFT_709_PENALTY=5.363671 PRIMER_RIGHT_709_PENALTY=2.219884 PRIMER_LEFT_709_SEQUENCE=TGAGTTTGTAAGTGATGCCCTTCT PRIMER_RIGHT_709_SEQUENCE=CCACATTGTCACTTTCTTCAGC PRIMER_LEFT_709=559,24 PRIMER_RIGHT_709=788,22 PRIMER_LEFT_709_TM=61.364 PRIMER_RIGHT_709_TM=59.780 PRIMER_LEFT_709_GC_PERCENT=41.667 PRIMER_RIGHT_709_GC_PERCENT=45.455 PRIMER_LEFT_709_SELF_ANY=4.00 PRIMER_RIGHT_709_SELF_ANY=5.00 PRIMER_LEFT_709_SELF_END=0.00 PRIMER_RIGHT_709_SELF_END=2.00 PRIMER_LEFT_709_END_STABILITY=6.7000 PRIMER_RIGHT_709_END_STABILITY=8.2000 PRIMER_PAIR_709_COMPL_ANY=6.00 PRIMER_PAIR_709_COMPL_END=1.00 PRIMER_PAIR_709_PRODUCT_SIZE=230 PRIMER_PAIR_710_PENALTY=7.663120 PRIMER_LEFT_710_PENALTY=3.496786 PRIMER_RIGHT_710_PENALTY=4.166335 PRIMER_LEFT_710_SEQUENCE=ACATGAATGTCCAGAATGGGAA PRIMER_RIGHT_710_SEQUENCE=AAGCAGCGGTAGGGAATC PRIMER_LEFT_710=330,22 PRIMER_RIGHT_710=552,18 PRIMER_LEFT_710_TM=61.497 PRIMER_RIGHT_710_TM=57.834 PRIMER_LEFT_710_GC_PERCENT=40.909 PRIMER_RIGHT_710_GC_PERCENT=55.556 PRIMER_LEFT_710_SELF_ANY=6.00 PRIMER_RIGHT_710_SELF_ANY=3.00 PRIMER_LEFT_710_SELF_END=1.00 PRIMER_RIGHT_710_SELF_END=3.00 PRIMER_LEFT_710_END_STABILITY=9.7000 PRIMER_RIGHT_710_END_STABILITY=6.6000 PRIMER_PAIR_710_COMPL_ANY=3.00 PRIMER_PAIR_710_COMPL_END=1.00 PRIMER_PAIR_710_PRODUCT_SIZE=223 PRIMER_PAIR_711_PENALTY=7.803086 PRIMER_LEFT_711_PENALTY=3.615453 PRIMER_RIGHT_711_PENALTY=4.187633 PRIMER_LEFT_711_SEQUENCE=CATGAATGTCCAGAATGGGAAG PRIMER_RIGHT_711_SEQUENCE=GCATCACTTACAAACTCACCAA PRIMER_LEFT_711=331,22 PRIMER_RIGHT_711=576,22 PRIMER_LEFT_711_TM=61.615 PRIMER_RIGHT_711_TM=57.812 PRIMER_LEFT_711_GC_PERCENT=45.455 PRIMER_RIGHT_711_GC_PERCENT=40.909 PRIMER_LEFT_711_SELF_ANY=4.00 PRIMER_RIGHT_711_SELF_ANY=2.00 PRIMER_LEFT_711_SELF_END=0.00 PRIMER_RIGHT_711_SELF_END=0.00 PRIMER_LEFT_711_END_STABILITY=8.2000 PRIMER_RIGHT_711_END_STABILITY=8.2000 PRIMER_PAIR_711_COMPL_ANY=3.00 PRIMER_PAIR_711_COMPL_END=2.00 PRIMER_PAIR_711_PRODUCT_SIZE=246 PRIMER_PAIR_712_PENALTY=7.817035 PRIMER_LEFT_712_PENALTY=3.657116 PRIMER_RIGHT_712_PENALTY=4.159919 PRIMER_LEFT_712_SEQUENCE=AATGGGAAGTGGGATTCAG PRIMER_RIGHT_712_SEQUENCE=AGAAGGGCATCACTTACAAACTC PRIMER_LEFT_712=344,19 PRIMER_RIGHT_712=582,23 PRIMER_LEFT_712_TM=57.343 PRIMER_RIGHT_712_TM=58.840 PRIMER_LEFT_712_GC_PERCENT=47.368 PRIMER_RIGHT_712_GC_PERCENT=43.478 PRIMER_LEFT_712_SELF_ANY=3.00 PRIMER_RIGHT_712_SELF_ANY=4.00 PRIMER_LEFT_712_SELF_END=1.00 PRIMER_RIGHT_712_SELF_END=0.00 PRIMER_LEFT_712_END_STABILITY=7.0000 PRIMER_RIGHT_712_END_STABILITY=6.4000 PRIMER_PAIR_712_COMPL_ANY=5.00 PRIMER_PAIR_712_COMPL_END=1.00 PRIMER_PAIR_712_PRODUCT_SIZE=239 PRIMER_PAIR_713_PENALTY=7.817035 PRIMER_LEFT_713_PENALTY=3.657116 PRIMER_RIGHT_713_PENALTY=4.159919 PRIMER_LEFT_713_SEQUENCE=CAGAATGGGAAGTGGGATT PRIMER_RIGHT_713_SEQUENCE=GAGAAGGGCATCACTTACAAACT PRIMER_LEFT_713=341,19 PRIMER_RIGHT_713=583,23 PRIMER_LEFT_713_TM=57.343 PRIMER_RIGHT_713_TM=58.840 PRIMER_LEFT_713_GC_PERCENT=47.368 PRIMER_RIGHT_713_GC_PERCENT=43.478 PRIMER_LEFT_713_SELF_ANY=3.00 PRIMER_RIGHT_713_SELF_ANY=4.00 PRIMER_LEFT_713_SELF_END=1.00 PRIMER_RIGHT_713_SELF_END=1.00 PRIMER_LEFT_713_END_STABILITY=8.1000 PRIMER_RIGHT_713_END_STABILITY=6.7000 PRIMER_PAIR_713_COMPL_ANY=5.00 PRIMER_PAIR_713_COMPL_END=1.00 PRIMER_PAIR_713_PRODUCT_SIZE=243 PRIMER_PAIR_714_PENALTY=7.859272 PRIMER_LEFT_714_PENALTY=5.725379 PRIMER_RIGHT_714_PENALTY=2.133893 PRIMER_LEFT_714_SEQUENCE=CGTTCCTGACAAGTGCAAATTCT PRIMER_RIGHT_714_SEQUENCE=GAGTCATCCTCCTCCGCA PRIMER_LEFT_714=583,23 PRIMER_RIGHT_714=816,18 PRIMER_LEFT_714_TM=62.725 PRIMER_RIGHT_714_TM=59.866 PRIMER_LEFT_714_GC_PERCENT=43.478 PRIMER_RIGHT_714_GC_PERCENT=61.111 PRIMER_LEFT_714_SELF_ANY=4.00 PRIMER_RIGHT_714_SELF_ANY=3.00 PRIMER_LEFT_714_SELF_END=2.00 PRIMER_RIGHT_714_SELF_END=2.00 PRIMER_LEFT_714_END_STABILITY=6.6000 PRIMER_RIGHT_714_END_STABILITY=11.7000 PRIMER_PAIR_714_COMPL_ANY=4.00 PRIMER_PAIR_714_COMPL_END=1.00 PRIMER_PAIR_714_PRODUCT_SIZE=234 PRIMER_PAIR_715_PENALTY=7.871200 PRIMER_LEFT_715_PENALTY=3.672773 PRIMER_RIGHT_715_PENALTY=4.198427 PRIMER_LEFT_715_SEQUENCE=TGAATGTCCAGAATGGGAA PRIMER_RIGHT_715_SEQUENCE=CAAACTCACCAACTAAGCAGCG PRIMER_LEFT_715=333,19 PRIMER_RIGHT_715=566,22 PRIMER_LEFT_715_TM=57.327 PRIMER_RIGHT_715_TM=62.198 PRIMER_LEFT_715_GC_PERCENT=42.105 PRIMER_RIGHT_715_GC_PERCENT=50.000 PRIMER_LEFT_715_SELF_ANY=3.00 PRIMER_RIGHT_715_SELF_ANY=3.00 PRIMER_LEFT_715_SELF_END=1.00 PRIMER_RIGHT_715_SELF_END=2.00 PRIMER_LEFT_715_END_STABILITY=9.7000 PRIMER_RIGHT_715_END_STABILITY=10.2000 PRIMER_PAIR_715_COMPL_ANY=3.00 PRIMER_PAIR_715_COMPL_END=0.00 PRIMER_PAIR_715_PRODUCT_SIZE=234 PRIMER_PAIR_716_PENALTY=7.977121 PRIMER_LEFT_716_PENALTY=5.713033 PRIMER_RIGHT_716_PENALTY=2.264088 PRIMER_LEFT_716_SEQUENCE=TGTAAGTGATGCCCTTCTCGTTC PRIMER_RIGHT_716_SEQUENCE=TGGGCAACACACAAACTCTA PRIMER_LEFT_716=565,23 PRIMER_RIGHT_716=763,20 PRIMER_LEFT_716_TM=62.713 PRIMER_RIGHT_716_TM=57.736 PRIMER_LEFT_716_GC_PERCENT=47.826 PRIMER_RIGHT_716_GC_PERCENT=45.000 PRIMER_LEFT_716_SELF_ANY=4.00 PRIMER_RIGHT_716_SELF_ANY=2.00 PRIMER_LEFT_716_SELF_END=2.00 PRIMER_RIGHT_716_SELF_END=2.00 PRIMER_LEFT_716_END_STABILITY=8.4000 PRIMER_RIGHT_716_END_STABILITY=5.7000 PRIMER_PAIR_716_COMPL_ANY=7.00 PRIMER_PAIR_716_COMPL_END=0.00 PRIMER_PAIR_716_PRODUCT_SIZE=199 PRIMER_PAIR_717_PENALTY=8.015748 PRIMER_LEFT_717_PENALTY=3.731444 PRIMER_RIGHT_717_PENALTY=4.284304 PRIMER_LEFT_717_SEQUENCE=TCAGATCCATCAGGGACCAAA PRIMER_RIGHT_717_SEQUENCE=AGGAACGAGAAGGGCATC PRIMER_LEFT_717=359,21 PRIMER_RIGHT_717=589,18 PRIMER_LEFT_717_TM=62.731 PRIMER_RIGHT_717_TM=57.716 PRIMER_LEFT_717_GC_PERCENT=47.619 PRIMER_RIGHT_717_GC_PERCENT=55.556 PRIMER_LEFT_717_SELF_ANY=5.00 PRIMER_RIGHT_717_SELF_ANY=2.00 PRIMER_LEFT_717_SELF_END=3.00 PRIMER_RIGHT_717_SELF_END=2.00 PRIMER_LEFT_717_END_STABILITY=8.8000 PRIMER_RIGHT_717_END_STABILITY=8.1000 PRIMER_PAIR_717_COMPL_ANY=3.00 PRIMER_PAIR_717_COMPL_END=0.00 PRIMER_PAIR_717_PRODUCT_SIZE=231 PRIMER_PAIR_718_PENALTY=8.024632 PRIMER_LEFT_718_PENALTY=3.731444 PRIMER_RIGHT_718_PENALTY=4.293188 PRIMER_LEFT_718_SEQUENCE=TTCAGATCCATCAGGGACCAA PRIMER_RIGHT_718_SEQUENCE=GGCATCACTTACAAACTCACCAA PRIMER_LEFT_718=358,21 PRIMER_RIGHT_718=577,23 PRIMER_LEFT_718_TM=62.731 PRIMER_RIGHT_718_TM=61.293 PRIMER_LEFT_718_GC_PERCENT=47.619 PRIMER_RIGHT_718_GC_PERCENT=43.478 PRIMER_LEFT_718_SELF_ANY=5.00 PRIMER_RIGHT_718_SELF_ANY=2.00 PRIMER_LEFT_718_SELF_END=1.00 PRIMER_RIGHT_718_SELF_END=0.00 PRIMER_LEFT_718_END_STABILITY=8.2000 PRIMER_RIGHT_718_END_STABILITY=8.2000 PRIMER_PAIR_718_COMPL_ANY=3.00 PRIMER_PAIR_718_COMPL_END=0.00 PRIMER_PAIR_718_PRODUCT_SIZE=220 PRIMER_PAIR_719_PENALTY=8.025488 PRIMER_LEFT_719_PENALTY=3.731877 PRIMER_RIGHT_719_PENALTY=4.293610 PRIMER_LEFT_719_SEQUENCE=GATCCATCAGGGACCAAAACC PRIMER_RIGHT_719_SEQUENCE=CTAAGCAGCGGTAGGGAATCAC PRIMER_LEFT_719=362,21 PRIMER_RIGHT_719=554,22 PRIMER_LEFT_719_TM=62.732 PRIMER_RIGHT_719_TM=62.294 PRIMER_LEFT_719_GC_PERCENT=52.381 PRIMER_RIGHT_719_GC_PERCENT=54.545 PRIMER_LEFT_719_SELF_ANY=5.00 PRIMER_RIGHT_719_SELF_ANY=3.00 PRIMER_LEFT_719_SELF_END=0.00 PRIMER_RIGHT_719_SELF_END=3.00 PRIMER_LEFT_719_END_STABILITY=8.2000 PRIMER_RIGHT_719_END_STABILITY=6.3000 PRIMER_PAIR_719_COMPL_ANY=3.00 PRIMER_PAIR_719_COMPL_END=0.00 PRIMER_PAIR_719_PRODUCT_SIZE=193 PRIMER_PAIR_720_PENALTY=8.079760 PRIMER_LEFT_720_PENALTY=5.781967 PRIMER_RIGHT_720_PENALTY=2.297793 PRIMER_LEFT_720_SEQUENCE=TGACAAGTGCAAATTCTTACACCA PRIMER_RIGHT_720_SEQUENCE=AGTGGGCAACACACAAACTCTA PRIMER_LEFT_720=589,24 PRIMER_RIGHT_720=765,22 PRIMER_LEFT_720_TM=61.782 PRIMER_RIGHT_720_TM=59.702 PRIMER_LEFT_720_GC_PERCENT=37.500 PRIMER_RIGHT_720_GC_PERCENT=45.455 PRIMER_LEFT_720_SELF_ANY=5.00 PRIMER_RIGHT_720_SELF_ANY=3.00 PRIMER_LEFT_720_SELF_END=1.00 PRIMER_RIGHT_720_SELF_END=2.00 PRIMER_LEFT_720_END_STABILITY=8.2000 PRIMER_RIGHT_720_END_STABILITY=5.7000 PRIMER_PAIR_720_COMPL_ANY=3.00 PRIMER_PAIR_720_COMPL_END=0.00 PRIMER_PAIR_720_PRODUCT_SIZE=177 PRIMER_PAIR_721_PENALTY=8.103842 PRIMER_LEFT_721_PENALTY=3.810232 PRIMER_RIGHT_721_PENALTY=4.293610 PRIMER_LEFT_721_SEQUENCE=CATGAATGTCCAGAATGGG PRIMER_RIGHT_721_SEQUENCE=ACTAAGCAGCGGTAGGGAATCA PRIMER_LEFT_721=331,19 PRIMER_RIGHT_721=555,22 PRIMER_LEFT_721_TM=57.190 PRIMER_RIGHT_721_TM=62.294 PRIMER_LEFT_721_GC_PERCENT=47.368 PRIMER_RIGHT_721_GC_PERCENT=50.000 PRIMER_LEFT_721_SELF_ANY=4.00 PRIMER_RIGHT_721_SELF_ANY=3.00 PRIMER_LEFT_721_SELF_END=1.00 PRIMER_RIGHT_721_SELF_END=1.00 PRIMER_LEFT_721_END_STABILITY=9.6000 PRIMER_RIGHT_721_END_STABILITY=6.9000 PRIMER_PAIR_721_COMPL_ANY=3.00 PRIMER_PAIR_721_COMPL_END=0.00 PRIMER_PAIR_721_PRODUCT_SIZE=225 PRIMER_PAIR_722_PENALTY=8.133235 PRIMER_LEFT_722_PENALTY=3.823109 PRIMER_RIGHT_722_PENALTY=4.310125 PRIMER_LEFT_722_SEQUENCE=ATCCATCAGGGACCAAAAC PRIMER_RIGHT_722_SEQUENCE=GCATCACTTACAAACTCACCAAC PRIMER_LEFT_722=363,19 PRIMER_RIGHT_722=576,23 PRIMER_LEFT_722_TM=57.177 PRIMER_RIGHT_722_TM=58.690 PRIMER_LEFT_722_GC_PERCENT=47.368 PRIMER_RIGHT_722_GC_PERCENT=43.478 PRIMER_LEFT_722_SELF_ANY=5.00 PRIMER_RIGHT_722_SELF_ANY=2.00 PRIMER_LEFT_722_SELF_END=0.00 PRIMER_RIGHT_722_SELF_END=0.00 PRIMER_LEFT_722_END_STABILITY=7.0000 PRIMER_RIGHT_722_END_STABILITY=8.2000 PRIMER_PAIR_722_COMPL_ANY=3.00 PRIMER_PAIR_722_COMPL_END=0.00 PRIMER_PAIR_722_PRODUCT_SIZE=214 PRIMER_PAIR_723_PENALTY=8.193379 PRIMER_LEFT_723_PENALTY=3.840048 PRIMER_RIGHT_723_PENALTY=4.353331 PRIMER_LEFT_723_SEQUENCE=CCAGAATGGGAAGTGGGATTC PRIMER_RIGHT_723_SEQUENCE=TACAAACTCACCAACTAAGCAGC PRIMER_LEFT_723=340,21 PRIMER_RIGHT_723=568,23 PRIMER_LEFT_723_TM=62.840 PRIMER_RIGHT_723_TM=58.647 PRIMER_LEFT_723_GC_PERCENT=52.381 PRIMER_RIGHT_723_GC_PERCENT=43.478 PRIMER_LEFT_723_SELF_ANY=4.00 PRIMER_RIGHT_723_SELF_ANY=3.00 PRIMER_LEFT_723_SELF_END=3.00 PRIMER_RIGHT_723_SELF_END=3.00 PRIMER_LEFT_723_END_STABILITY=6.6000 PRIMER_RIGHT_723_END_STABILITY=9.7000 PRIMER_PAIR_723_COMPL_ANY=4.00 PRIMER_PAIR_723_COMPL_END=1.00 PRIMER_PAIR_723_PRODUCT_SIZE=229 PRIMER_PAIR_724_PENALTY=8.210802 PRIMER_LEFT_724_PENALTY=3.840048 PRIMER_RIGHT_724_PENALTY=4.370754 PRIMER_LEFT_724_SEQUENCE=TCCAGAATGGGAAGTGGGATT PRIMER_RIGHT_724_SEQUENCE=GCATCACTTACAAACTCACCAACT PRIMER_LEFT_724=339,21 PRIMER_RIGHT_724=576,24 PRIMER_LEFT_724_TM=62.840 PRIMER_RIGHT_724_TM=59.629 PRIMER_LEFT_724_GC_PERCENT=47.619 PRIMER_RIGHT_724_GC_PERCENT=41.667 PRIMER_LEFT_724_SELF_ANY=3.00 PRIMER_RIGHT_724_SELF_ANY=2.00 PRIMER_LEFT_724_SELF_END=1.00 PRIMER_RIGHT_724_SELF_END=1.00 PRIMER_LEFT_724_END_STABILITY=8.1000 PRIMER_RIGHT_724_END_STABILITY=6.7000 PRIMER_PAIR_724_COMPL_ANY=5.00 PRIMER_PAIR_724_COMPL_END=3.00 PRIMER_PAIR_724_PRODUCT_SIZE=238 PRIMER_PAIR_725_PENALTY=8.291522 PRIMER_LEFT_725_PENALTY=3.864768 PRIMER_RIGHT_725_PENALTY=4.426754 PRIMER_LEFT_725_SEQUENCE=CAAGAAGTCTACCCTGAACTGC PRIMER_RIGHT_725_SEQUENCE=TAAGAATTTGCACTTGTCAGGA PRIMER_LEFT_725=419,22 PRIMER_RIGHT_725=607,22 PRIMER_LEFT_725_TM=58.135 PRIMER_RIGHT_725_TM=57.573 PRIMER_LEFT_725_GC_PERCENT=50.000 PRIMER_RIGHT_725_GC_PERCENT=36.364 PRIMER_LEFT_725_SELF_ANY=4.00 PRIMER_RIGHT_725_SELF_ANY=4.00 PRIMER_LEFT_725_SELF_END=2.00 PRIMER_RIGHT_725_SELF_END=2.00 PRIMER_LEFT_725_END_STABILITY=7.9000 PRIMER_RIGHT_725_END_STABILITY=8.2000 PRIMER_PAIR_725_COMPL_ANY=5.00 PRIMER_PAIR_725_COMPL_END=3.00 PRIMER_PAIR_725_PRODUCT_SIZE=189 PRIMER_PAIR_726_PENALTY=8.295537 PRIMER_LEFT_726_PENALTY=6.353121 PRIMER_RIGHT_726_PENALTY=1.942416 PRIMER_LEFT_726_SEQUENCE=TTCTTACACCAGGAGAGGATGGAT PRIMER_RIGHT_726_SEQUENCE=ATAGTCTGTGTCTGCTCCGC PRIMER_LEFT_726=602,24 PRIMER_RIGHT_726=850,20 PRIMER_LEFT_726_TM=62.353 PRIMER_RIGHT_726_TM=58.058 PRIMER_LEFT_726_GC_PERCENT=45.833 PRIMER_RIGHT_726_GC_PERCENT=55.000 PRIMER_LEFT_726_SELF_ANY=3.00 PRIMER_RIGHT_726_SELF_ANY=3.00 PRIMER_LEFT_726_SELF_END=2.00 PRIMER_RIGHT_726_SELF_END=2.00 PRIMER_LEFT_726_END_STABILITY=8.1000 PRIMER_RIGHT_726_END_STABILITY=11.4000 PRIMER_PAIR_726_COMPL_ANY=4.00 PRIMER_PAIR_726_COMPL_END=1.00 PRIMER_PAIR_726_PRODUCT_SIZE=249 PRIMER_PAIR_727_PENALTY=8.295879 PRIMER_LEFT_727_PENALTY=3.883954 PRIMER_RIGHT_727_PENALTY=4.411925 PRIMER_LEFT_727_SEQUENCE=ATCAGGGACCAAAACCTGCAT PRIMER_RIGHT_727_SEQUENCE=AACTCACCAACTAAGCAGCGGT PRIMER_LEFT_727=367,21 PRIMER_RIGHT_727=564,22 PRIMER_LEFT_727_TM=62.884 PRIMER_RIGHT_727_TM=62.412 PRIMER_LEFT_727_GC_PERCENT=47.619 PRIMER_RIGHT_727_GC_PERCENT=50.000 PRIMER_LEFT_727_SELF_ANY=4.00 PRIMER_RIGHT_727_SELF_ANY=3.00 PRIMER_LEFT_727_SELF_END=2.00 PRIMER_RIGHT_727_SELF_END=2.00 PRIMER_LEFT_727_END_STABILITY=8.4000 PRIMER_RIGHT_727_END_STABILITY=11.1000 PRIMER_PAIR_727_COMPL_ANY=4.00 PRIMER_PAIR_727_COMPL_END=3.00 PRIMER_PAIR_727_PRODUCT_SIZE=198 PRIMER_PAIR_728_PENALTY=8.389490 PRIMER_LEFT_728_PENALTY=5.997786 PRIMER_RIGHT_728_PENALTY=2.391704 PRIMER_LEFT_728_SEQUENCE=GTTGGTGAGTTTGTAAGTGATGCC PRIMER_RIGHT_728_SEQUENCE=CATCAGCAGAATCCACATTG PRIMER_LEFT_728=554,24 PRIMER_RIGHT_728=800,20 PRIMER_LEFT_728_TM=61.998 PRIMER_RIGHT_728_TM=57.608 PRIMER_LEFT_728_GC_PERCENT=45.833 PRIMER_RIGHT_728_GC_PERCENT=45.000 PRIMER_LEFT_728_SELF_ANY=2.00 PRIMER_RIGHT_728_SELF_ANY=4.00 PRIMER_LEFT_728_SELF_END=1.00 PRIMER_RIGHT_728_SELF_END=3.00 PRIMER_LEFT_728_END_STABILITY=9.6000 PRIMER_RIGHT_728_END_STABILITY=7.2000 PRIMER_PAIR_728_COMPL_ANY=5.00 PRIMER_PAIR_728_COMPL_END=1.00 PRIMER_PAIR_728_PRODUCT_SIZE=247 PRIMER_PAIR_729_PENALTY=8.495577 PRIMER_LEFT_729_PENALTY=4.008330 PRIMER_RIGHT_729_PENALTY=4.487247 PRIMER_LEFT_729_SEQUENCE=CCAAGAAGTCTACCCTGAACTG PRIMER_RIGHT_729_SEQUENCE=CTGGTGTAAGAATTTGCACTTG PRIMER_LEFT_729=418,22 PRIMER_RIGHT_729=613,22 PRIMER_LEFT_729_TM=57.992 PRIMER_RIGHT_729_TM=57.513 PRIMER_LEFT_729_GC_PERCENT=50.000 PRIMER_RIGHT_729_GC_PERCENT=40.909 PRIMER_LEFT_729_SELF_ANY=4.00 PRIMER_RIGHT_729_SELF_ANY=5.00 PRIMER_LEFT_729_SELF_END=1.00 PRIMER_RIGHT_729_SELF_END=3.00 PRIMER_LEFT_729_END_STABILITY=6.7000 PRIMER_RIGHT_729_END_STABILITY=6.7000 PRIMER_PAIR_729_COMPL_ANY=4.00 PRIMER_PAIR_729_COMPL_END=2.00 PRIMER_PAIR_729_PRODUCT_SIZE=196 PRIMER_PAIR_730_PENALTY=8.592124 PRIMER_LEFT_730_PENALTY=4.070165 PRIMER_RIGHT_730_PENALTY=4.521959 PRIMER_LEFT_730_SEQUENCE=AGAATGGGAAGTGGGATTCAGA PRIMER_RIGHT_730_SEQUENCE=GAGAAGGGCATCACTTACAAACTC PRIMER_LEFT_730=342,22 PRIMER_RIGHT_730=583,24 PRIMER_LEFT_730_TM=62.070 PRIMER_RIGHT_730_TM=60.522 PRIMER_LEFT_730_GC_PERCENT=45.455 PRIMER_RIGHT_730_GC_PERCENT=45.833 PRIMER_LEFT_730_SELF_ANY=4.00 PRIMER_RIGHT_730_SELF_ANY=4.00 PRIMER_LEFT_730_SELF_END=3.00 PRIMER_RIGHT_730_SELF_END=0.00 PRIMER_LEFT_730_END_STABILITY=6.7000 PRIMER_RIGHT_730_END_STABILITY=6.4000 PRIMER_PAIR_730_COMPL_ANY=5.00 PRIMER_PAIR_730_COMPL_END=2.00 PRIMER_PAIR_730_PRODUCT_SIZE=242 PRIMER_PAIR_731_PENALTY=8.664179 PRIMER_LEFT_731_PENALTY=6.353121 PRIMER_RIGHT_731_PENALTY=2.311058 PRIMER_LEFT_731_SEQUENCE=ATTCTTACACCAGGAGAGGATGGA PRIMER_RIGHT_731_SEQUENCE=TGGGCAACACACAAACTCTAC PRIMER_LEFT_731=601,24 PRIMER_RIGHT_731=763,21 PRIMER_LEFT_731_TM=62.353 PRIMER_RIGHT_731_TM=58.689 PRIMER_LEFT_731_GC_PERCENT=45.833 PRIMER_RIGHT_731_GC_PERCENT=47.619 PRIMER_LEFT_731_SELF_ANY=3.00 PRIMER_RIGHT_731_SELF_ANY=2.00 PRIMER_LEFT_731_SELF_END=0.00 PRIMER_RIGHT_731_SELF_END=0.00 PRIMER_LEFT_731_END_STABILITY=8.1000 PRIMER_RIGHT_731_END_STABILITY=5.4000 PRIMER_PAIR_731_COMPL_ANY=4.00 PRIMER_PAIR_731_COMPL_END=2.00 PRIMER_PAIR_731_PRODUCT_SIZE=163 PRIMER_PAIR_732_PENALTY=8.779292 PRIMER_LEFT_732_PENALTY=4.186531 PRIMER_RIGHT_732_PENALTY=4.592761 PRIMER_LEFT_732_SEQUENCE=TGAATGTCCAGAATGGGAAGTG PRIMER_RIGHT_732_SEQUENCE=AGCGGTAGGGAATCACAAAGTG PRIMER_LEFT_732=333,22 PRIMER_RIGHT_732=548,22 PRIMER_LEFT_732_TM=62.187 PRIMER_RIGHT_732_TM=62.593 PRIMER_LEFT_732_GC_PERCENT=45.455 PRIMER_RIGHT_732_GC_PERCENT=50.000 PRIMER_LEFT_732_SELF_ANY=3.00 PRIMER_RIGHT_732_SELF_ANY=3.00 PRIMER_LEFT_732_SELF_END=0.00 PRIMER_RIGHT_732_SELF_END=3.00 PRIMER_LEFT_732_END_STABILITY=6.7000 PRIMER_RIGHT_732_END_STABILITY=6.7000 PRIMER_PAIR_732_COMPL_ANY=3.00 PRIMER_PAIR_732_COMPL_END=0.00 PRIMER_PAIR_732_PRODUCT_SIZE=216 PRIMER_PAIR_733_PENALTY=8.793065 PRIMER_LEFT_733_PENALTY=4.232394 PRIMER_RIGHT_733_PENALTY=4.560670 PRIMER_LEFT_733_SEQUENCE=CAGATCCATCAGGGACCA PRIMER_RIGHT_733_SEQUENCE=GTAAGAATTTGCACTTGTCAGGA PRIMER_LEFT_733=360,18 PRIMER_RIGHT_733=608,23 PRIMER_LEFT_733_TM=57.768 PRIMER_RIGHT_733_TM=58.439 PRIMER_LEFT_733_GC_PERCENT=55.556 PRIMER_RIGHT_733_GC_PERCENT=39.130 PRIMER_LEFT_733_SELF_ANY=5.00 PRIMER_RIGHT_733_SELF_ANY=4.00 PRIMER_LEFT_733_SELF_END=3.00 PRIMER_RIGHT_733_SELF_END=2.00 PRIMER_LEFT_733_END_STABILITY=7.9000 PRIMER_RIGHT_733_END_STABILITY=8.2000 PRIMER_PAIR_733_COMPL_ANY=3.00 PRIMER_PAIR_733_COMPL_END=1.00 PRIMER_PAIR_733_PRODUCT_SIZE=249 PRIMER_PAIR_734_PENALTY=8.838174 PRIMER_LEFT_734_PENALTY=6.527116 PRIMER_RIGHT_734_PENALTY=2.311058 PRIMER_LEFT_734_SEQUENCE=CAAGTGCAAATTCTTACACCAGGA PRIMER_RIGHT_734_SEQUENCE=GTGGGCAACACACAAACTCTA PRIMER_LEFT_734=592,24 PRIMER_RIGHT_734=764,21 PRIMER_LEFT_734_TM=62.527 PRIMER_RIGHT_734_TM=58.689 PRIMER_LEFT_734_GC_PERCENT=41.667 PRIMER_RIGHT_734_GC_PERCENT=47.619 PRIMER_LEFT_734_SELF_ANY=5.00 PRIMER_RIGHT_734_SELF_ANY=3.00 PRIMER_LEFT_734_SELF_END=1.00 PRIMER_RIGHT_734_SELF_END=2.00 PRIMER_LEFT_734_END_STABILITY=8.2000 PRIMER_RIGHT_734_END_STABILITY=5.7000 PRIMER_PAIR_734_COMPL_ANY=3.00 PRIMER_PAIR_734_COMPL_END=0.00 PRIMER_PAIR_734_PRODUCT_SIZE=173 PRIMER_PAIR_735_PENALTY=9.101354 PRIMER_LEFT_735_PENALTY=4.508593 PRIMER_RIGHT_735_PENALTY=4.592761 PRIMER_LEFT_735_SEQUENCE=AGTGGGATTCAGATCCATCAGG PRIMER_RIGHT_735_SEQUENCE=CAGCGGTAGGGAATCACAAAGT PRIMER_LEFT_735=351,22 PRIMER_RIGHT_735=549,22 PRIMER_LEFT_735_TM=62.509 PRIMER_RIGHT_735_TM=62.593 PRIMER_LEFT_735_GC_PERCENT=50.000 PRIMER_RIGHT_735_GC_PERCENT=50.000 PRIMER_LEFT_735_SELF_ANY=5.00 PRIMER_RIGHT_735_SELF_ANY=3.00 PRIMER_LEFT_735_SELF_END=2.00 PRIMER_RIGHT_735_SELF_END=1.00 PRIMER_LEFT_735_END_STABILITY=8.2000 PRIMER_RIGHT_735_END_STABILITY=6.7000 PRIMER_PAIR_735_COMPL_ANY=6.00 PRIMER_PAIR_735_COMPL_END=0.00 PRIMER_PAIR_735_PRODUCT_SIZE=199 PRIMER_PAIR_736_PENALTY=9.139055 PRIMER_LEFT_736_PENALTY=4.514227 PRIMER_RIGHT_736_PENALTY=4.624828 PRIMER_LEFT_736_SEQUENCE=CCAAGAAGTCTACCCTGAACTGC PRIMER_RIGHT_736_SEQUENCE=CTGGTGTAAGAATTTGCACTTGT PRIMER_LEFT_736=418,23 PRIMER_RIGHT_736=613,23 PRIMER_LEFT_736_TM=61.514 PRIMER_RIGHT_736_TM=58.375 PRIMER_LEFT_736_GC_PERCENT=52.174 PRIMER_RIGHT_736_GC_PERCENT=39.130 PRIMER_LEFT_736_SELF_ANY=4.00 PRIMER_RIGHT_736_SELF_ANY=5.00 PRIMER_LEFT_736_SELF_END=2.00 PRIMER_RIGHT_736_SELF_END=2.00 PRIMER_LEFT_736_END_STABILITY=7.9000 PRIMER_RIGHT_736_END_STABILITY=6.7000 PRIMER_PAIR_736_COMPL_ANY=4.00 PRIMER_PAIR_736_COMPL_END=2.00 PRIMER_PAIR_736_PRODUCT_SIZE=196 PRIMER_PAIR_737_PENALTY=9.146536 PRIMER_LEFT_737_PENALTY=4.514227 PRIMER_RIGHT_737_PENALTY=4.632309 PRIMER_LEFT_737_SEQUENCE=GCCAAGAAGTCTACCCTGAACTG PRIMER_RIGHT_737_SEQUENCE=AGGGCATCACTTACAAACTCACC PRIMER_LEFT_737=417,23 PRIMER_RIGHT_737=579,23 PRIMER_LEFT_737_TM=61.514 PRIMER_RIGHT_737_TM=61.632 PRIMER_LEFT_737_GC_PERCENT=52.174 PRIMER_RIGHT_737_GC_PERCENT=47.826 PRIMER_LEFT_737_SELF_ANY=4.00 PRIMER_RIGHT_737_SELF_ANY=2.00 PRIMER_LEFT_737_SELF_END=1.00 PRIMER_RIGHT_737_SELF_END=0.00 PRIMER_LEFT_737_END_STABILITY=6.7000 PRIMER_RIGHT_737_END_STABILITY=7.9000 PRIMER_PAIR_737_COMPL_ANY=5.00 PRIMER_PAIR_737_COMPL_END=1.00 PRIMER_PAIR_737_PRODUCT_SIZE=163 PRIMER_PAIR_738_PENALTY=9.150610 PRIMER_LEFT_738_PENALTY=4.514227 PRIMER_RIGHT_738_PENALTY=4.636384 PRIMER_LEFT_738_SEQUENCE=TGCCAAGAAGTCTACCCTGAACT PRIMER_RIGHT_738_SEQUENCE=TGGTGTAAGAATTTGCACTTGT PRIMER_LEFT_738=416,23 PRIMER_RIGHT_738=612,22 PRIMER_LEFT_738_TM=61.514 PRIMER_RIGHT_738_TM=57.364 PRIMER_LEFT_738_GC_PERCENT=47.826 PRIMER_RIGHT_738_GC_PERCENT=36.364 PRIMER_LEFT_738_SELF_ANY=4.00 PRIMER_RIGHT_738_SELF_ANY=5.00 PRIMER_LEFT_738_SELF_END=1.00 PRIMER_RIGHT_738_SELF_END=2.00 PRIMER_LEFT_738_END_STABILITY=6.4000 PRIMER_RIGHT_738_END_STABILITY=6.7000 PRIMER_PAIR_738_COMPL_ANY=4.00 PRIMER_PAIR_738_COMPL_END=1.00 PRIMER_PAIR_738_PRODUCT_SIZE=197 PRIMER_PAIR_739_PENALTY=9.167757 PRIMER_LEFT_739_PENALTY=6.765645 PRIMER_RIGHT_739_PENALTY=2.402111 PRIMER_LEFT_739_SEQUENCE=CTGCTTAGTTGGTGAGTTTGTAAGT PRIMER_RIGHT_739_SEQUENCE=CTTCAGCCAGTGGGCAAC PRIMER_LEFT_739=547,25 PRIMER_RIGHT_739=773,18 PRIMER_LEFT_739_TM=58.234 PRIMER_RIGHT_739_TM=60.402 PRIMER_LEFT_739_GC_PERCENT=40.000 PRIMER_RIGHT_739_GC_PERCENT=61.111 PRIMER_LEFT_739_SELF_ANY=4.00 PRIMER_RIGHT_739_SELF_ANY=5.00 PRIMER_LEFT_739_SELF_END=1.00 PRIMER_RIGHT_739_SELF_END=3.00 PRIMER_LEFT_739_END_STABILITY=5.7000 PRIMER_RIGHT_739_END_STABILITY=8.2000 PRIMER_PAIR_739_COMPL_ANY=4.00 PRIMER_PAIR_739_COMPL_END=2.00 PRIMER_PAIR_739_PRODUCT_SIZE=227 PRIMER_PAIR_740_PENALTY=9.219852 PRIMER_LEFT_740_PENALTY=4.625552 PRIMER_RIGHT_740_PENALTY=4.594300 PRIMER_LEFT_740_SEQUENCE=TGGGAAGTGGGATTCAGA PRIMER_RIGHT_740_SEQUENCE=CACCAACTAAGCAGCGGT PRIMER_LEFT_740=346,18 PRIMER_RIGHT_740=560,18 PRIMER_LEFT_740_TM=57.374 PRIMER_RIGHT_740_TM=57.406 PRIMER_LEFT_740_GC_PERCENT=50.000 PRIMER_RIGHT_740_GC_PERCENT=55.556 PRIMER_LEFT_740_SELF_ANY=3.00 PRIMER_RIGHT_740_SELF_ANY=3.00 PRIMER_LEFT_740_SELF_END=3.00 PRIMER_RIGHT_740_SELF_END=2.00 PRIMER_LEFT_740_END_STABILITY=6.7000 PRIMER_RIGHT_740_END_STABILITY=11.1000 PRIMER_PAIR_740_COMPL_ANY=4.00 PRIMER_PAIR_740_COMPL_END=1.00 PRIMER_PAIR_740_PRODUCT_SIZE=215 PRIMER_PAIR_741_PENALTY=9.225005 PRIMER_LEFT_741_PENALTY=6.910331 PRIMER_RIGHT_741_PENALTY=2.314674 PRIMER_LEFT_741_SEQUENCE=GCAAATTCTTACACCAGGAGAGGA PRIMER_RIGHT_741_SEQUENCE=CATCCTCCTCCGCATCAG PRIMER_LEFT_741=597,24 PRIMER_RIGHT_741=812,18 PRIMER_LEFT_741_TM=62.910 PRIMER_RIGHT_741_TM=60.315 PRIMER_LEFT_741_GC_PERCENT=45.833 PRIMER_RIGHT_741_GC_PERCENT=61.111 PRIMER_LEFT_741_SELF_ANY=4.00 PRIMER_RIGHT_741_SELF_ANY=2.00 PRIMER_LEFT_741_SELF_END=3.00 PRIMER_RIGHT_741_SELF_END=2.00 PRIMER_LEFT_741_END_STABILITY=7.9000 PRIMER_RIGHT_741_END_STABILITY=6.6000 PRIMER_PAIR_741_COMPL_ANY=6.00 PRIMER_PAIR_741_COMPL_END=3.00 PRIMER_PAIR_741_PRODUCT_SIZE=216 PRIMER_PAIR_742_PENALTY=9.270801 PRIMER_LEFT_742_PENALTY=6.837687 PRIMER_RIGHT_742_PENALTY=2.433114 PRIMER_LEFT_742_SEQUENCE=GTTTGTAAGTGATGCCCTTCTCGT PRIMER_RIGHT_742_SEQUENCE=GTGGGCAACACACAAACTCTAC PRIMER_LEFT_742=562,24 PRIMER_RIGHT_742=764,22 PRIMER_LEFT_742_TM=62.838 PRIMER_RIGHT_742_TM=59.567 PRIMER_LEFT_742_GC_PERCENT=45.833 PRIMER_RIGHT_742_GC_PERCENT=50.000 PRIMER_LEFT_742_SELF_ANY=4.00 PRIMER_RIGHT_742_SELF_ANY=3.00 PRIMER_LEFT_742_SELF_END=2.00 PRIMER_RIGHT_742_SELF_END=0.00 PRIMER_LEFT_742_END_STABILITY=8.1000 PRIMER_RIGHT_742_END_STABILITY=5.4000 PRIMER_PAIR_742_COMPL_ANY=7.00 PRIMER_PAIR_742_COMPL_END=2.00 PRIMER_PAIR_742_PRODUCT_SIZE=203 PRIMER_PAIR_743_PENALTY=9.324254 PRIMER_LEFT_743_PENALTY=6.798336 PRIMER_RIGHT_743_PENALTY=2.525918 PRIMER_LEFT_743_SEQUENCE=TTAGTTGGTGAGTTTGTAAGTGATGC PRIMER_RIGHT_743_SEQUENCE=ATCAGCAGAATCCACATTGTCA PRIMER_LEFT_743=551,26 PRIMER_RIGHT_743=799,22 PRIMER_LEFT_743_TM=60.798 PRIMER_RIGHT_743_TM=60.526 PRIMER_LEFT_743_GC_PERCENT=38.462 PRIMER_RIGHT_743_GC_PERCENT=40.909 PRIMER_LEFT_743_SELF_ANY=3.00 PRIMER_RIGHT_743_SELF_ANY=5.00 PRIMER_LEFT_743_SELF_END=2.00 PRIMER_RIGHT_743_SELF_END=3.00 PRIMER_LEFT_743_END_STABILITY=8.1000 PRIMER_RIGHT_743_END_STABILITY=6.7000 PRIMER_PAIR_743_COMPL_ANY=4.00 PRIMER_PAIR_743_COMPL_END=2.00 PRIMER_PAIR_743_PRODUCT_SIZE=249 PRIMER_PAIR_744_PENALTY=9.459408 PRIMER_LEFT_744_PENALTY=6.933490 PRIMER_RIGHT_744_PENALTY=2.525918 PRIMER_LEFT_744_SEQUENCE=AGTTTGTAAGTGATGCCCTTCTCG PRIMER_RIGHT_744_SEQUENCE=CATCAGCAGAATCCACATTGTC PRIMER_LEFT_744=561,24 PRIMER_RIGHT_744=800,22 PRIMER_LEFT_744_TM=62.933 PRIMER_RIGHT_744_TM=60.526 PRIMER_LEFT_744_GC_PERCENT=45.833 PRIMER_RIGHT_744_GC_PERCENT=45.455 PRIMER_LEFT_744_SELF_ANY=4.00 PRIMER_RIGHT_744_SELF_ANY=5.00 PRIMER_LEFT_744_SELF_END=2.00 PRIMER_RIGHT_744_SELF_END=3.00 PRIMER_LEFT_744_END_STABILITY=8.4000 PRIMER_RIGHT_744_END_STABILITY=6.7000 PRIMER_PAIR_744_COMPL_ANY=5.00 PRIMER_PAIR_744_COMPL_END=1.00 PRIMER_PAIR_744_PRODUCT_SIZE=240 PRIMER_PAIR_745_PENALTY=9.541298 PRIMER_LEFT_745_PENALTY=4.856179 PRIMER_RIGHT_745_PENALTY=4.685119 PRIMER_LEFT_745_SEQUENCE=ATTCAGATCCATCAGGGACCAA PRIMER_RIGHT_745_SEQUENCE=GGAACGAGAAGGGCATCACTTA PRIMER_LEFT_745=357,22 PRIMER_RIGHT_745=588,22 PRIMER_LEFT_745_TM=62.856 PRIMER_RIGHT_745_TM=62.685 PRIMER_LEFT_745_GC_PERCENT=45.455 PRIMER_RIGHT_745_GC_PERCENT=50.000 PRIMER_LEFT_745_SELF_ANY=5.00 PRIMER_RIGHT_745_SELF_ANY=4.00 PRIMER_LEFT_745_SELF_END=1.00 PRIMER_RIGHT_745_SELF_END=2.00 PRIMER_LEFT_745_END_STABILITY=8.2000 PRIMER_RIGHT_745_END_STABILITY=5.7000 PRIMER_PAIR_745_COMPL_ANY=3.00 PRIMER_PAIR_745_COMPL_END=1.00 PRIMER_PAIR_745_PRODUCT_SIZE=232 PRIMER_PAIR_746_PENALTY=9.563447 PRIMER_LEFT_746_PENALTY=7.131558 PRIMER_RIGHT_746_PENALTY=2.431889 PRIMER_LEFT_746_SEQUENCE=AGTGCAAATTCTTACACCAGGAGAG PRIMER_RIGHT_746_SEQUENCE=CCAGTGGGCAACACACAAAC PRIMER_LEFT_746=594,25 PRIMER_RIGHT_746=767,20 PRIMER_LEFT_746_TM=62.132 PRIMER_RIGHT_746_TM=62.432 PRIMER_LEFT_746_GC_PERCENT=44.000 PRIMER_RIGHT_746_GC_PERCENT=55.000 PRIMER_LEFT_746_SELF_ANY=5.00 PRIMER_RIGHT_746_SELF_ANY=5.00 PRIMER_LEFT_746_SELF_END=0.00 PRIMER_RIGHT_746_SELF_END=0.00 PRIMER_LEFT_746_END_STABILITY=6.4000 PRIMER_RIGHT_746_END_STABILITY=7.0000 PRIMER_PAIR_746_COMPL_ANY=3.00 PRIMER_PAIR_746_COMPL_END=1.00 PRIMER_PAIR_746_PRODUCT_SIZE=174 PRIMER_PAIR_747_PENALTY=9.618247 PRIMER_LEFT_747_PENALTY=7.141896 PRIMER_RIGHT_747_PENALTY=2.476351 PRIMER_LEFT_747_SEQUENCE=TTCCTGACAAGTGCAAATTCTTACA PRIMER_RIGHT_747_SEQUENCE=CAGTGGGCAACACACAAAC PRIMER_LEFT_747=585,25 PRIMER_RIGHT_747=766,19 PRIMER_LEFT_747_TM=62.142 PRIMER_RIGHT_747_TM=58.524 PRIMER_LEFT_747_GC_PERCENT=36.000 PRIMER_RIGHT_747_GC_PERCENT=52.632 PRIMER_LEFT_747_SELF_ANY=4.00 PRIMER_RIGHT_747_SELF_ANY=3.00 PRIMER_LEFT_747_SELF_END=3.00 PRIMER_RIGHT_747_SELF_END=0.00 PRIMER_LEFT_747_END_STABILITY=6.0000 PRIMER_RIGHT_747_END_STABILITY=7.0000 PRIMER_PAIR_747_COMPL_ANY=3.00 PRIMER_PAIR_747_COMPL_END=0.00 PRIMER_PAIR_747_PRODUCT_SIZE=182 PRIMER_PAIR_748_PENALTY=9.623396 PRIMER_LEFT_748_PENALTY=7.951017 PRIMER_RIGHT_748_PENALTY=1.672380 PRIMER_LEFT_748_SEQUENCE=GATGTTTGCGAAACTCATCTTCACT PRIMER_RIGHT_748_SEQUENCE=TGTCTTCACTCCCATCTGCAT PRIMER_LEFT_748=623,25 PRIMER_RIGHT_748=869,21 PRIMER_LEFT_748_TM=62.951 PRIMER_RIGHT_748_TM=60.672 PRIMER_LEFT_748_GC_PERCENT=40.000 PRIMER_RIGHT_748_GC_PERCENT=47.619 PRIMER_LEFT_748_SELF_ANY=6.00 PRIMER_RIGHT_748_SELF_ANY=4.00 PRIMER_LEFT_748_SELF_END=1.00 PRIMER_RIGHT_748_SELF_END=2.00 PRIMER_LEFT_748_END_STABILITY=6.4000 PRIMER_RIGHT_748_END_STABILITY=8.4000 PRIMER_PAIR_748_COMPL_ANY=4.00 PRIMER_PAIR_748_COMPL_END=0.00 PRIMER_PAIR_748_PRODUCT_SIZE=247 PRIMER_PAIR_749_PENALTY=9.625521 PRIMER_LEFT_749_PENALTY=4.867932 PRIMER_RIGHT_749_PENALTY=4.757589 PRIMER_LEFT_749_SEQUENCE=CAGATCCATCAGGGACCAAAAC PRIMER_RIGHT_749_SEQUENCE=TGCACTTGTCAGGAACGA PRIMER_LEFT_749=360,22 PRIMER_RIGHT_749=599,18 PRIMER_LEFT_749_TM=62.868 PRIMER_RIGHT_749_TM=57.242 PRIMER_LEFT_749_GC_PERCENT=50.000 PRIMER_RIGHT_749_GC_PERCENT=50.000 PRIMER_LEFT_749_SELF_ANY=5.00 PRIMER_RIGHT_749_SELF_ANY=4.00 PRIMER_LEFT_749_SELF_END=0.00 PRIMER_RIGHT_749_SELF_END=0.00 PRIMER_LEFT_749_END_STABILITY=7.0000 PRIMER_RIGHT_749_END_STABILITY=8.4000 PRIMER_PAIR_749_COMPL_ANY=3.00 PRIMER_PAIR_749_COMPL_END=0.00 PRIMER_PAIR_749_PRODUCT_SIZE=240 PRIMER_PAIR_750_PENALTY=9.635571 PRIMER_LEFT_750_PENALTY=7.031344 PRIMER_RIGHT_750_PENALTY=2.604227 PRIMER_LEFT_750_SEQUENCE=GTGAGTTTGTAAGTGATGCCCTTCT PRIMER_RIGHT_750_SEQUENCE=GCATCAGCAGAATCCACATTG PRIMER_LEFT_750=558,25 PRIMER_RIGHT_750=801,21 PRIMER_LEFT_750_TM=62.031 PRIMER_RIGHT_750_TM=61.604 PRIMER_LEFT_750_GC_PERCENT=44.000 PRIMER_RIGHT_750_GC_PERCENT=47.619 PRIMER_LEFT_750_SELF_ANY=4.00 PRIMER_RIGHT_750_SELF_ANY=4.00 PRIMER_LEFT_750_SELF_END=0.00 PRIMER_RIGHT_750_SELF_END=3.00 PRIMER_LEFT_750_END_STABILITY=6.7000 PRIMER_RIGHT_750_END_STABILITY=7.2000 PRIMER_PAIR_750_COMPL_ANY=6.00 PRIMER_PAIR_750_COMPL_END=1.00 PRIMER_PAIR_750_PRODUCT_SIZE=244 PRIMER_PAIR_751_PENALTY=9.678772 PRIMER_LEFT_751_PENALTY=4.860451 PRIMER_RIGHT_751_PENALTY=4.818321 PRIMER_LEFT_751_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_751_SEQUENCE=TGCACTTGTCAGGAACGAGAAG PRIMER_LEFT_751=412,24 PRIMER_RIGHT_751=599,22 PRIMER_LEFT_751_TM=60.860 PRIMER_RIGHT_751_TM=62.818 PRIMER_LEFT_751_GC_PERCENT=45.833 PRIMER_RIGHT_751_GC_PERCENT=50.000 PRIMER_LEFT_751_SELF_ANY=5.00 PRIMER_RIGHT_751_SELF_ANY=4.00 PRIMER_LEFT_751_SELF_END=1.00 PRIMER_RIGHT_751_SELF_END=3.00 PRIMER_LEFT_751_END_STABILITY=8.2000 PRIMER_RIGHT_751_END_STABILITY=6.7000 PRIMER_PAIR_751_COMPL_ANY=5.00 PRIMER_PAIR_751_COMPL_END=0.00 PRIMER_PAIR_751_PRODUCT_SIZE=188 PRIMER_PAIR_752_PENALTY=9.709783 PRIMER_LEFT_752_PENALTY=4.867932 PRIMER_RIGHT_752_PENALTY=4.841851 PRIMER_LEFT_752_SEQUENCE=GAATGTCCAGAATGGGAAGTGG PRIMER_RIGHT_752_SEQUENCE=AAACTCACCAACTAAGCAGCGG PRIMER_LEFT_752=334,22 PRIMER_RIGHT_752=565,22 PRIMER_LEFT_752_TM=62.868 PRIMER_RIGHT_752_TM=62.842 PRIMER_LEFT_752_GC_PERCENT=50.000 PRIMER_RIGHT_752_GC_PERCENT=50.000 PRIMER_LEFT_752_SELF_ANY=3.00 PRIMER_RIGHT_752_SELF_ANY=3.00 PRIMER_LEFT_752_SELF_END=0.00 PRIMER_RIGHT_752_SELF_END=2.00 PRIMER_LEFT_752_END_STABILITY=7.9000 PRIMER_RIGHT_752_END_STABILITY=11.4000 PRIMER_PAIR_752_COMPL_ANY=4.00 PRIMER_PAIR_752_COMPL_END=0.00 PRIMER_PAIR_752_PRODUCT_SIZE=232 PRIMER_PAIR_753_PENALTY=9.851488 PRIMER_LEFT_753_PENALTY=7.298929 PRIMER_RIGHT_753_PENALTY=2.552559 PRIMER_LEFT_753_SEQUENCE=CAAATTCTTACACCAGGAGAGGATG PRIMER_RIGHT_753_SEQUENCE=CCCACCAGACATCCGAGT PRIMER_LEFT_753=598,25 PRIMER_RIGHT_753=830,18 PRIMER_LEFT_753_TM=62.299 PRIMER_RIGHT_753_TM=59.447 PRIMER_LEFT_753_GC_PERCENT=44.000 PRIMER_RIGHT_753_GC_PERCENT=61.111 PRIMER_LEFT_753_SELF_ANY=4.00 PRIMER_RIGHT_753_SELF_ANY=3.00 PRIMER_LEFT_753_SELF_END=0.00 PRIMER_RIGHT_753_SELF_END=3.00 PRIMER_LEFT_753_END_STABILITY=8.1000 PRIMER_RIGHT_753_END_STABILITY=8.1000 PRIMER_PAIR_753_COMPL_ANY=5.00 PRIMER_PAIR_753_COMPL_END=2.00 PRIMER_PAIR_753_PRODUCT_SIZE=233 PRIMER_PAIR_754_PENALTY=9.912956 PRIMER_LEFT_754_PENALTY=4.973389 PRIMER_RIGHT_754_PENALTY=4.939568 PRIMER_LEFT_754_SEQUENCE=CAGAATGGGAAGTGGGATTCAG PRIMER_RIGHT_754_SEQUENCE=AGAAGGGCATCACTTACAAACT PRIMER_LEFT_754=341,22 PRIMER_RIGHT_754=582,22 PRIMER_LEFT_754_TM=62.973 PRIMER_RIGHT_754_TM=57.060 PRIMER_LEFT_754_GC_PERCENT=50.000 PRIMER_RIGHT_754_GC_PERCENT=40.909 PRIMER_LEFT_754_SELF_ANY=4.00 PRIMER_RIGHT_754_SELF_ANY=4.00 PRIMER_LEFT_754_SELF_END=2.00 PRIMER_RIGHT_754_SELF_END=1.00 PRIMER_LEFT_754_END_STABILITY=7.0000 PRIMER_RIGHT_754_END_STABILITY=6.7000 PRIMER_PAIR_754_COMPL_ANY=5.00 PRIMER_PAIR_754_COMPL_END=2.00 PRIMER_PAIR_754_PRODUCT_SIZE=242 PRIMER_PAIR_755_PENALTY=10.000121 PRIMER_LEFT_755_PENALTY=7.384820 PRIMER_RIGHT_755_PENALTY=2.615302 PRIMER_LEFT_755_SEQUENCE=TGCTTAGTTGGTGAGTTTGTAAGTGA PRIMER_RIGHT_755_SEQUENCE=CCAGTGGGCAACACACAAA PRIMER_LEFT_755=548,26 PRIMER_RIGHT_755=767,19 PRIMER_LEFT_755_TM=61.385 PRIMER_RIGHT_755_TM=61.615 PRIMER_LEFT_755_GC_PERCENT=38.462 PRIMER_RIGHT_755_GC_PERCENT=52.632 PRIMER_LEFT_755_SELF_ANY=4.00 PRIMER_RIGHT_755_SELF_ANY=5.00 PRIMER_LEFT_755_SELF_END=1.00 PRIMER_RIGHT_755_SELF_END=0.00 PRIMER_LEFT_755_END_STABILITY=6.4000 PRIMER_RIGHT_755_END_STABILITY=7.0000 PRIMER_PAIR_755_COMPL_ANY=6.00 PRIMER_PAIR_755_COMPL_END=3.00 PRIMER_PAIR_755_PRODUCT_SIZE=220 PRIMER_PAIR_756_PENALTY=10.032035 PRIMER_LEFT_756_PENALTY=7.438416 PRIMER_RIGHT_756_PENALTY=2.593620 PRIMER_LEFT_756_SEQUENCE=AAGTGCAAATTCTTACACCAGGAGA PRIMER_RIGHT_756_SEQUENCE=CAGTGGGCAACACACAAACTC PRIMER_LEFT_756=593,25 PRIMER_RIGHT_756=766,21 PRIMER_LEFT_756_TM=62.438 PRIMER_RIGHT_756_TM=61.594 PRIMER_LEFT_756_GC_PERCENT=40.000 PRIMER_RIGHT_756_GC_PERCENT=52.381 PRIMER_LEFT_756_SELF_ANY=5.00 PRIMER_RIGHT_756_SELF_ANY=3.00 PRIMER_LEFT_756_SELF_END=1.00 PRIMER_RIGHT_756_SELF_END=0.00 PRIMER_LEFT_756_END_STABILITY=7.9000 PRIMER_RIGHT_756_END_STABILITY=6.4000 PRIMER_PAIR_756_COMPL_ANY=3.00 PRIMER_PAIR_756_COMPL_END=2.00 PRIMER_PAIR_756_PRODUCT_SIZE=174 PRIMER_PAIR_757_PENALTY=10.067159 PRIMER_LEFT_757_PENALTY=7.480183 PRIMER_RIGHT_757_PENALTY=2.586975 PRIMER_LEFT_757_SEQUENCE=ATTCTTACACCAGGAGAGGATGGAT PRIMER_RIGHT_757_SEQUENCE=GTCTGTGTCTGCTCCGCC PRIMER_LEFT_757=601,25 PRIMER_RIGHT_757=847,18 PRIMER_LEFT_757_TM=62.480 PRIMER_RIGHT_757_TM=60.587 PRIMER_LEFT_757_GC_PERCENT=44.000 PRIMER_RIGHT_757_GC_PERCENT=66.667 PRIMER_LEFT_757_SELF_ANY=3.00 PRIMER_RIGHT_757_SELF_ANY=2.00 PRIMER_LEFT_757_SELF_END=2.00 PRIMER_RIGHT_757_SELF_END=1.00 PRIMER_LEFT_757_END_STABILITY=8.1000 PRIMER_RIGHT_757_END_STABILITY=12.9000 PRIMER_PAIR_757_COMPL_ANY=4.00 PRIMER_PAIR_757_COMPL_END=1.00 PRIMER_PAIR_757_PRODUCT_SIZE=247 PRIMER_PAIR_758_PENALTY=10.117496 PRIMER_LEFT_758_PENALTY=7.358003 PRIMER_RIGHT_758_PENALTY=2.759493 PRIMER_LEFT_758_SEQUENCE=GCTGCTTAGTTGGTGAGTTTGTAAGT PRIMER_RIGHT_758_SEQUENCE=AGCCAGTGGGCAACACAC PRIMER_LEFT_758=546,26 PRIMER_RIGHT_758=769,18 PRIMER_LEFT_758_TM=61.358 PRIMER_RIGHT_758_TM=60.759 PRIMER_LEFT_758_GC_PERCENT=42.308 PRIMER_RIGHT_758_GC_PERCENT=61.111 PRIMER_LEFT_758_SELF_ANY=4.00 PRIMER_RIGHT_758_SELF_ANY=5.00 PRIMER_LEFT_758_SELF_END=1.00 PRIMER_RIGHT_758_SELF_END=3.00 PRIMER_LEFT_758_END_STABILITY=5.7000 PRIMER_RIGHT_758_END_STABILITY=6.4000 PRIMER_PAIR_758_COMPL_ANY=5.00 PRIMER_PAIR_758_COMPL_END=2.00 PRIMER_PAIR_758_PRODUCT_SIZE=224 PRIMER_PAIR_759_PENALTY=10.128958 PRIMER_LEFT_759_PENALTY=5.291723 PRIMER_RIGHT_759_PENALTY=4.837235 PRIMER_LEFT_759_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_759_SEQUENCE=TGTCAGGAACGAGAAGGG PRIMER_LEFT_759=413,24 PRIMER_RIGHT_759=593,18 PRIMER_LEFT_759_TM=61.292 PRIMER_RIGHT_759_TM=57.163 PRIMER_LEFT_759_GC_PERCENT=41.667 PRIMER_RIGHT_759_GC_PERCENT=55.556 PRIMER_LEFT_759_SELF_ANY=5.00 PRIMER_RIGHT_759_SELF_ANY=3.00 PRIMER_LEFT_759_SELF_END=0.00 PRIMER_RIGHT_759_SELF_END=0.00 PRIMER_LEFT_759_END_STABILITY=7.0000 PRIMER_RIGHT_759_END_STABILITY=9.7000 PRIMER_PAIR_759_COMPL_ANY=5.00 PRIMER_PAIR_759_COMPL_END=1.00 PRIMER_PAIR_759_PRODUCT_SIZE=181 PRIMER_PAIR_760_PENALTY=10.202636 PRIMER_LEFT_760_PENALTY=5.219386 PRIMER_RIGHT_760_PENALTY=4.983250 PRIMER_LEFT_760_SEQUENCE=AGAATGGGAAGTGGGATTCAGAT PRIMER_RIGHT_760_SEQUENCE=GCGGTAGGGAATCACAAA PRIMER_LEFT_760=342,23 PRIMER_RIGHT_760=547,18 PRIMER_LEFT_760_TM=62.219 PRIMER_RIGHT_760_TM=57.017 PRIMER_LEFT_760_GC_PERCENT=43.478 PRIMER_RIGHT_760_GC_PERCENT=50.000 PRIMER_LEFT_760_SELF_ANY=4.00 PRIMER_RIGHT_760_SELF_ANY=3.00 PRIMER_LEFT_760_SELF_END=2.00 PRIMER_RIGHT_760_SELF_END=1.00 PRIMER_LEFT_760_END_STABILITY=6.6000 PRIMER_RIGHT_760_END_STABILITY=7.0000 PRIMER_PAIR_760_COMPL_ANY=6.00 PRIMER_PAIR_760_COMPL_END=2.00 PRIMER_PAIR_760_PRODUCT_SIZE=206 PRIMER_PAIR_761_PENALTY=10.320870 PRIMER_LEFT_761_PENALTY=5.335483 PRIMER_RIGHT_761_PENALTY=4.985387 PRIMER_LEFT_761_SEQUENCE=ATGAATGTCCAGAATGGGAAGTG PRIMER_RIGHT_761_SEQUENCE=TGGGGATGGGTCTTGCAC PRIMER_LEFT_761=332,23 PRIMER_RIGHT_761=528,18 PRIMER_LEFT_761_TM=62.335 PRIMER_RIGHT_761_TM=62.985 PRIMER_LEFT_761_GC_PERCENT=43.478 PRIMER_RIGHT_761_GC_PERCENT=61.111 PRIMER_LEFT_761_SELF_ANY=3.00 PRIMER_RIGHT_761_SELF_ANY=4.00 PRIMER_LEFT_761_SELF_END=0.00 PRIMER_RIGHT_761_SELF_END=2.00 PRIMER_LEFT_761_END_STABILITY=6.7000 PRIMER_RIGHT_761_END_STABILITY=8.2000 PRIMER_PAIR_761_COMPL_ANY=3.00 PRIMER_PAIR_761_COMPL_END=3.00 PRIMER_PAIR_761_PRODUCT_SIZE=197 PRIMER_PAIR_762_PENALTY=10.327516 PRIMER_LEFT_762_PENALTY=7.470065 PRIMER_RIGHT_762_PENALTY=2.857451 PRIMER_LEFT_762_SEQUENCE=TTAGTTGGTGAGTTTGTAAGTGATG PRIMER_RIGHT_762_SEQUENCE=AAACTCTACCCCTCGGAACTTG PRIMER_LEFT_762=551,25 PRIMER_RIGHT_762=751,22 PRIMER_LEFT_762_TM=57.530 PRIMER_RIGHT_762_TM=60.857 PRIMER_LEFT_762_GC_PERCENT=36.000 PRIMER_RIGHT_762_GC_PERCENT=50.000 PRIMER_LEFT_762_SELF_ANY=3.00 PRIMER_RIGHT_762_SELF_ANY=3.00 PRIMER_LEFT_762_SELF_END=0.00 PRIMER_RIGHT_762_SELF_END=1.00 PRIMER_LEFT_762_END_STABILITY=6.9000 PRIMER_RIGHT_762_END_STABILITY=6.7000 PRIMER_PAIR_762_COMPL_ANY=7.00 PRIMER_PAIR_762_COMPL_END=1.00 PRIMER_PAIR_762_PRODUCT_SIZE=201 PRIMER_PAIR_763_PENALTY=10.334806 PRIMER_LEFT_763_PENALTY=7.554905 PRIMER_RIGHT_763_PENALTY=2.779901 PRIMER_LEFT_763_SEQUENCE=TGACAAGTGCAAATTCTTACACCAG PRIMER_RIGHT_763_SEQUENCE=CTCCTCCGCATCAGCAGAAT PRIMER_LEFT_763=589,25 PRIMER_RIGHT_763=808,20 PRIMER_LEFT_763_TM=62.555 PRIMER_RIGHT_763_TM=62.780 PRIMER_LEFT_763_GC_PERCENT=40.000 PRIMER_RIGHT_763_GC_PERCENT=55.000 PRIMER_LEFT_763_SELF_ANY=5.00 PRIMER_RIGHT_763_SELF_ANY=4.00 PRIMER_LEFT_763_SELF_END=3.00 PRIMER_RIGHT_763_SELF_END=2.00 PRIMER_LEFT_763_END_STABILITY=7.9000 PRIMER_RIGHT_763_END_STABILITY=6.6000 PRIMER_PAIR_763_COMPL_ANY=5.00 PRIMER_PAIR_763_COMPL_END=0.00 PRIMER_PAIR_763_PRODUCT_SIZE=220 PRIMER_PAIR_764_PENALTY=10.352207 PRIMER_LEFT_764_PENALTY=5.335483 PRIMER_RIGHT_764_PENALTY=5.016724 PRIMER_LEFT_764_SEQUENCE=CATGAATGTCCAGAATGGGAAGT PRIMER_RIGHT_764_SEQUENCE=ACTCACCAACTAAGCAGCGGTAG PRIMER_LEFT_764=331,23 PRIMER_RIGHT_764=563,23 PRIMER_LEFT_764_TM=62.335 PRIMER_RIGHT_764_TM=62.017 PRIMER_LEFT_764_GC_PERCENT=43.478 PRIMER_RIGHT_764_GC_PERCENT=52.174 PRIMER_LEFT_764_SELF_ANY=4.00 PRIMER_RIGHT_764_SELF_ANY=3.00 PRIMER_LEFT_764_SELF_END=1.00 PRIMER_RIGHT_764_SELF_END=3.00 PRIMER_LEFT_764_END_STABILITY=6.4000 PRIMER_RIGHT_764_END_STABILITY=6.9000 PRIMER_PAIR_764_COMPL_ANY=4.00 PRIMER_PAIR_764_COMPL_END=0.00 PRIMER_PAIR_764_PRODUCT_SIZE=233 PRIMER_PAIR_765_PENALTY=10.405221 PRIMER_LEFT_765_PENALTY=7.547770 PRIMER_RIGHT_765_PENALTY=2.857451 PRIMER_LEFT_765_SEQUENCE=CTTAGTTGGTGAGTTTGTAAGTGATG PRIMER_RIGHT_765_SEQUENCE=CAAACTCTACCCCTCGGAACTT PRIMER_LEFT_765=550,26 PRIMER_RIGHT_765=752,22 PRIMER_LEFT_765_TM=58.452 PRIMER_RIGHT_765_TM=60.857 PRIMER_LEFT_765_GC_PERCENT=38.462 PRIMER_RIGHT_765_GC_PERCENT=50.000 PRIMER_LEFT_765_SELF_ANY=4.00 PRIMER_RIGHT_765_SELF_ANY=3.00 PRIMER_LEFT_765_SELF_END=0.00 PRIMER_RIGHT_765_SELF_END=3.00 PRIMER_LEFT_765_END_STABILITY=6.9000 PRIMER_RIGHT_765_END_STABILITY=6.7000 PRIMER_PAIR_765_COMPL_ANY=8.00 PRIMER_PAIR_765_COMPL_END=2.00 PRIMER_PAIR_765_PRODUCT_SIZE=203 PRIMER_PAIR_766_PENALTY=10.438841 PRIMER_LEFT_766_PENALTY=5.409997 PRIMER_RIGHT_766_PENALTY=5.028844 PRIMER_LEFT_766_SEQUENCE=AGTATTGCCAAGAAGTCTACCCT PRIMER_RIGHT_766_SEQUENCE=TGTAAGAATTTGCACTTGTCAGGA PRIMER_LEFT_766=411,23 PRIMER_RIGHT_766=609,24 PRIMER_LEFT_766_TM=57.590 PRIMER_RIGHT_766_TM=61.029 PRIMER_LEFT_766_GC_PERCENT=43.478 PRIMER_RIGHT_766_GC_PERCENT=37.500 PRIMER_LEFT_766_SELF_ANY=5.00 PRIMER_RIGHT_766_SELF_ANY=4.00 PRIMER_LEFT_766_SELF_END=2.00 PRIMER_RIGHT_766_SELF_END=2.00 PRIMER_LEFT_766_END_STABILITY=9.1000 PRIMER_RIGHT_766_END_STABILITY=8.2000 PRIMER_PAIR_766_COMPL_ANY=5.00 PRIMER_PAIR_766_COMPL_END=2.00 PRIMER_PAIR_766_PRODUCT_SIZE=199 PRIMER_PAIR_767_PENALTY=10.444589 PRIMER_LEFT_767_PENALTY=7.554905 PRIMER_RIGHT_767_PENALTY=2.889684 PRIMER_LEFT_767_SEQUENCE=CTGACAAGTGCAAATTCTTACACCA PRIMER_RIGHT_767_SEQUENCE=CTCCGCATCAGCAGAATCC PRIMER_LEFT_767=588,25 PRIMER_RIGHT_767=805,19 PRIMER_LEFT_767_TM=62.555 PRIMER_RIGHT_767_TM=61.890 PRIMER_LEFT_767_GC_PERCENT=40.000 PRIMER_RIGHT_767_GC_PERCENT=57.895 PRIMER_LEFT_767_SELF_ANY=5.00 PRIMER_RIGHT_767_SELF_ANY=4.00 PRIMER_LEFT_767_SELF_END=1.00 PRIMER_RIGHT_767_SELF_END=2.00 PRIMER_LEFT_767_END_STABILITY=8.2000 PRIMER_RIGHT_767_END_STABILITY=8.1000 PRIMER_PAIR_767_COMPL_ANY=5.00 PRIMER_PAIR_767_COMPL_END=0.00 PRIMER_PAIR_767_PRODUCT_SIZE=218 PRIMER_PAIR_768_PENALTY=10.507809 PRIMER_LEFT_768_PENALTY=7.886984 PRIMER_RIGHT_768_PENALTY=2.620825 PRIMER_LEFT_768_SEQUENCE=AAATTCTTACACCAGGAGAGGATGG PRIMER_RIGHT_768_SEQUENCE=AGTCTGTGTCTGCTCCGCC PRIMER_LEFT_768=599,25 PRIMER_RIGHT_768=848,19 PRIMER_LEFT_768_TM=62.887 PRIMER_RIGHT_768_TM=61.621 PRIMER_LEFT_768_GC_PERCENT=44.000 PRIMER_RIGHT_768_GC_PERCENT=63.158 PRIMER_LEFT_768_SELF_ANY=4.00 PRIMER_RIGHT_768_SELF_ANY=3.00 PRIMER_LEFT_768_SELF_END=0.00 PRIMER_RIGHT_768_SELF_END=1.00 PRIMER_LEFT_768_END_STABILITY=8.1000 PRIMER_RIGHT_768_END_STABILITY=12.9000 PRIMER_PAIR_768_COMPL_ANY=4.00 PRIMER_PAIR_768_COMPL_END=2.00 PRIMER_PAIR_768_PRODUCT_SIZE=250 PRIMER_PAIR_769_PENALTY=10.529548 PRIMER_LEFT_769_PENALTY=5.335483 PRIMER_RIGHT_769_PENALTY=5.194065 PRIMER_LEFT_769_SEQUENCE=ACATGAATGTCCAGAATGGGAAG PRIMER_RIGHT_769_SEQUENCE=CACTTACAAACTCACCAACTAAGCA PRIMER_LEFT_769=330,23 PRIMER_RIGHT_769=572,25 PRIMER_LEFT_769_TM=62.335 PRIMER_RIGHT_769_TM=59.806 PRIMER_LEFT_769_GC_PERCENT=43.478 PRIMER_RIGHT_769_GC_PERCENT=40.000 PRIMER_LEFT_769_SELF_ANY=6.00 PRIMER_RIGHT_769_SELF_ANY=4.00 PRIMER_LEFT_769_SELF_END=0.00 PRIMER_RIGHT_769_SELF_END=0.00 PRIMER_LEFT_769_END_STABILITY=8.2000 PRIMER_RIGHT_769_END_STABILITY=8.5000 PRIMER_PAIR_769_COMPL_ANY=3.00 PRIMER_PAIR_769_COMPL_END=1.00 PRIMER_PAIR_769_PRODUCT_SIZE=243 PRIMER_PAIR_770_PENALTY=10.618447 PRIMER_LEFT_770_PENALTY=7.751085 PRIMER_RIGHT_770_PENALTY=2.867362 PRIMER_LEFT_770_SEQUENCE=AGTTGGTGAGTTTGTAAGTGATGCC PRIMER_RIGHT_770_SEQUENCE=TCAGCAGAATCCACATTGTC PRIMER_LEFT_770=553,25 PRIMER_RIGHT_770=798,20 PRIMER_LEFT_770_TM=62.751 PRIMER_RIGHT_770_TM=57.133 PRIMER_LEFT_770_GC_PERCENT=44.000 PRIMER_RIGHT_770_GC_PERCENT=45.000 PRIMER_LEFT_770_SELF_ANY=2.00 PRIMER_RIGHT_770_SELF_ANY=5.00 PRIMER_LEFT_770_SELF_END=1.00 PRIMER_RIGHT_770_SELF_END=3.00 PRIMER_LEFT_770_END_STABILITY=9.6000 PRIMER_RIGHT_770_END_STABILITY=6.7000 PRIMER_PAIR_770_COMPL_ANY=4.00 PRIMER_PAIR_770_COMPL_END=1.00 PRIMER_PAIR_770_PRODUCT_SIZE=246 PRIMER_PAIR_771_PENALTY=10.824667 PRIMER_LEFT_771_PENALTY=7.934983 PRIMER_RIGHT_771_PENALTY=2.889684 PRIMER_LEFT_771_SEQUENCE=TGAGTTTGTAAGTGATGCCCTTCTC PRIMER_RIGHT_771_SEQUENCE=CCTCCGCATCAGCAGAATC PRIMER_LEFT_771=559,25 PRIMER_RIGHT_771=806,19 PRIMER_LEFT_771_TM=62.935 PRIMER_RIGHT_771_TM=61.890 PRIMER_LEFT_771_GC_PERCENT=44.000 PRIMER_RIGHT_771_GC_PERCENT=57.895 PRIMER_LEFT_771_SELF_ANY=4.00 PRIMER_RIGHT_771_SELF_ANY=4.00 PRIMER_LEFT_771_SELF_END=2.00 PRIMER_RIGHT_771_SELF_END=3.00 PRIMER_LEFT_771_END_STABILITY=6.7000 PRIMER_RIGHT_771_END_STABILITY=6.6000 PRIMER_PAIR_771_COMPL_ANY=6.00 PRIMER_PAIR_771_COMPL_END=1.00 PRIMER_PAIR_771_PRODUCT_SIZE=248 PRIMER_PAIR_772_PENALTY=11.012563 PRIMER_LEFT_772_PENALTY=5.821907 PRIMER_RIGHT_772_PENALTY=5.190656 PRIMER_LEFT_772_SEQUENCE=TGCAGTATTGCCAAGAAGTCTACC PRIMER_RIGHT_772_SEQUENCE=CGAGAAGGGCATCACTTACAAAC PRIMER_LEFT_772=408,24 PRIMER_RIGHT_772=584,23 PRIMER_LEFT_772_TM=61.822 PRIMER_RIGHT_772_TM=62.191 PRIMER_LEFT_772_GC_PERCENT=45.833 PRIMER_RIGHT_772_GC_PERCENT=47.826 PRIMER_LEFT_772_SELF_ANY=6.00 PRIMER_RIGHT_772_SELF_ANY=4.00 PRIMER_LEFT_772_SELF_END=1.00 PRIMER_RIGHT_772_SELF_END=0.00 PRIMER_LEFT_772_END_STABILITY=6.9000 PRIMER_RIGHT_772_END_STABILITY=7.0000 PRIMER_PAIR_772_COMPL_ANY=5.00 PRIMER_PAIR_772_COMPL_END=0.00 PRIMER_PAIR_772_PRODUCT_SIZE=177 PRIMER_PAIR_773_PENALTY=11.191086 PRIMER_LEFT_773_PENALTY=6.000430 PRIMER_RIGHT_773_PENALTY=5.190656 PRIMER_LEFT_773_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_773_SEQUENCE=ACGAGAAGGGCATCACTTACAAA PRIMER_LEFT_773=414,24 PRIMER_RIGHT_773=585,23 PRIMER_LEFT_773_TM=62.000 PRIMER_RIGHT_773_TM=62.191 PRIMER_LEFT_773_GC_PERCENT=45.833 PRIMER_RIGHT_773_GC_PERCENT=43.478 PRIMER_LEFT_773_SELF_ANY=5.00 PRIMER_RIGHT_773_SELF_ANY=4.00 PRIMER_LEFT_773_SELF_END=0.00 PRIMER_RIGHT_773_SELF_END=1.00 PRIMER_LEFT_773_END_STABILITY=6.7000 PRIMER_RIGHT_773_END_STABILITY=7.0000 PRIMER_PAIR_773_COMPL_ANY=5.00 PRIMER_PAIR_773_COMPL_END=0.00 PRIMER_PAIR_773_PRODUCT_SIZE=172 PRIMER_PAIR_774_PENALTY=11.441555 PRIMER_LEFT_774_PENALTY=8.548866 PRIMER_RIGHT_774_PENALTY=2.892689 PRIMER_LEFT_774_SEQUENCE=CTTAGTTGGTGAGTTTGTAAGTGATGC PRIMER_RIGHT_774_SEQUENCE=ACTCTACCCCTCGGAACTTGTC PRIMER_LEFT_774=550,27 PRIMER_RIGHT_774=749,22 PRIMER_LEFT_774_TM=61.549 PRIMER_RIGHT_774_TM=60.893 PRIMER_LEFT_774_GC_PERCENT=40.741 PRIMER_RIGHT_774_GC_PERCENT=54.545 PRIMER_LEFT_774_SELF_ANY=4.00 PRIMER_RIGHT_774_SELF_ANY=3.00 PRIMER_LEFT_774_SELF_END=2.00 PRIMER_RIGHT_774_SELF_END=1.00 PRIMER_LEFT_774_END_STABILITY=8.1000 PRIMER_RIGHT_774_END_STABILITY=6.7000 PRIMER_PAIR_774_COMPL_ANY=5.00 PRIMER_PAIR_774_COMPL_END=0.00 PRIMER_PAIR_774_PRODUCT_SIZE=200 PRIMER_PAIR_775_PENALTY=11.444445 PRIMER_LEFT_775_PENALTY=8.548866 PRIMER_RIGHT_775_PENALTY=2.895578 PRIMER_LEFT_775_SEQUENCE=GCTTAGTTGGTGAGTTTGTAAGTGATG PRIMER_RIGHT_775_SEQUENCE=GCAGAATCCACATTGTCACTTT PRIMER_LEFT_775=549,27 PRIMER_RIGHT_775=795,22 PRIMER_LEFT_775_TM=61.549 PRIMER_RIGHT_775_TM=59.104 PRIMER_LEFT_775_GC_PERCENT=40.741 PRIMER_RIGHT_775_GC_PERCENT=40.909 PRIMER_LEFT_775_SELF_ANY=4.00 PRIMER_RIGHT_775_SELF_ANY=5.00 PRIMER_LEFT_775_SELF_END=0.00 PRIMER_RIGHT_775_SELF_END=3.00 PRIMER_LEFT_775_END_STABILITY=6.9000 PRIMER_RIGHT_775_END_STABILITY=6.7000 PRIMER_PAIR_775_COMPL_ANY=6.00 PRIMER_PAIR_775_COMPL_END=3.00 PRIMER_PAIR_775_PRODUCT_SIZE=247 PRIMER_PAIR_776_PENALTY=11.491456 PRIMER_LEFT_776_PENALTY=8.548866 PRIMER_RIGHT_776_PENALTY=2.942589 PRIMER_LEFT_776_SEQUENCE=TGCTTAGTTGGTGAGTTTGTAAGTGAT PRIMER_RIGHT_776_SEQUENCE=TCTACCCCTCGGAACTTGTCA PRIMER_LEFT_776=548,27 PRIMER_RIGHT_776=747,21 PRIMER_LEFT_776_TM=61.549 PRIMER_RIGHT_776_TM=61.943 PRIMER_LEFT_776_GC_PERCENT=37.037 PRIMER_RIGHT_776_GC_PERCENT=52.381 PRIMER_LEFT_776_SELF_ANY=4.00 PRIMER_RIGHT_776_SELF_ANY=3.00 PRIMER_LEFT_776_SELF_END=2.00 PRIMER_RIGHT_776_SELF_END=3.00 PRIMER_LEFT_776_END_STABILITY=6.3000 PRIMER_RIGHT_776_END_STABILITY=6.7000 PRIMER_PAIR_776_COMPL_ANY=4.00 PRIMER_PAIR_776_COMPL_END=2.00 PRIMER_PAIR_776_PRODUCT_SIZE=200 PRIMER_PAIR_777_PENALTY=11.629422 PRIMER_LEFT_777_PENALTY=6.438766 PRIMER_RIGHT_777_PENALTY=5.190656 PRIMER_LEFT_777_SEQUENCE=GCAGTATTGCCAAGAAGTCTACCC PRIMER_RIGHT_777_SEQUENCE=AACGAGAAGGGCATCACTTACAA PRIMER_LEFT_777=409,24 PRIMER_RIGHT_777=586,23 PRIMER_LEFT_777_TM=62.439 PRIMER_RIGHT_777_TM=62.191 PRIMER_LEFT_777_GC_PERCENT=50.000 PRIMER_RIGHT_777_GC_PERCENT=43.478 PRIMER_LEFT_777_SELF_ANY=6.00 PRIMER_RIGHT_777_SELF_ANY=4.00 PRIMER_LEFT_777_SELF_END=0.00 PRIMER_RIGHT_777_SELF_END=2.00 PRIMER_LEFT_777_END_STABILITY=8.4000 PRIMER_RIGHT_777_END_STABILITY=6.0000 PRIMER_PAIR_777_COMPL_ANY=5.00 PRIMER_PAIR_777_COMPL_END=1.00 PRIMER_PAIR_777_PRODUCT_SIZE=178 PRIMER_PAIR_778_PENALTY=11.652859 PRIMER_LEFT_778_PENALTY=8.671003 PRIMER_RIGHT_778_PENALTY=2.981856 PRIMER_LEFT_778_SEQUENCE=TAGTTGGTGAGTTTGTAAGTGATGCC PRIMER_RIGHT_778_SEQUENCE=ACCCCTCGGAACTTGTCA PRIMER_LEFT_778=552,26 PRIMER_RIGHT_778=744,18 PRIMER_LEFT_778_TM=62.671 PRIMER_RIGHT_778_TM=59.018 PRIMER_LEFT_778_GC_PERCENT=42.308 PRIMER_RIGHT_778_GC_PERCENT=55.556 PRIMER_LEFT_778_SELF_ANY=2.00 PRIMER_RIGHT_778_SELF_ANY=3.00 PRIMER_LEFT_778_SELF_END=1.00 PRIMER_RIGHT_778_SELF_END=3.00 PRIMER_LEFT_778_END_STABILITY=9.6000 PRIMER_RIGHT_778_END_STABILITY=6.7000 PRIMER_PAIR_778_COMPL_ANY=4.00 PRIMER_PAIR_778_COMPL_END=2.00 PRIMER_PAIR_778_PRODUCT_SIZE=193 PRIMER_PAIR_779_PENALTY=11.656166 PRIMER_LEFT_779_PENALTY=8.766482 PRIMER_RIGHT_779_PENALTY=2.889684 PRIMER_LEFT_779_SEQUENCE=GTTCCTGACAAGTGCAAATTCTTACA PRIMER_RIGHT_779_SEQUENCE=TCCTCCGCATCAGCAGAAT PRIMER_LEFT_779=584,26 PRIMER_RIGHT_779=807,19 PRIMER_LEFT_779_TM=62.766 PRIMER_RIGHT_779_TM=61.890 PRIMER_LEFT_779_GC_PERCENT=38.462 PRIMER_RIGHT_779_GC_PERCENT=52.632 PRIMER_LEFT_779_SELF_ANY=4.00 PRIMER_RIGHT_779_SELF_ANY=4.00 PRIMER_LEFT_779_SELF_END=3.00 PRIMER_RIGHT_779_SELF_END=2.00 PRIMER_LEFT_779_END_STABILITY=6.0000 PRIMER_RIGHT_779_END_STABILITY=6.6000 PRIMER_PAIR_779_COMPL_ANY=5.00 PRIMER_PAIR_779_COMPL_END=2.00 PRIMER_PAIR_779_PRODUCT_SIZE=224 PRIMER_PAIR_780_PENALTY=11.864150 PRIMER_LEFT_780_PENALTY=6.610485 PRIMER_RIGHT_780_PENALTY=5.253665 PRIMER_LEFT_780_SEQUENCE=TTGCCAAGAAGTCTACCCTGAACT PRIMER_RIGHT_780_SEQUENCE=GAAGGGCATCACTTACAAACTCAC PRIMER_LEFT_780=415,24 PRIMER_RIGHT_780=581,24 PRIMER_LEFT_780_TM=62.610 PRIMER_RIGHT_780_TM=61.254 PRIMER_LEFT_780_GC_PERCENT=45.833 PRIMER_RIGHT_780_GC_PERCENT=45.833 PRIMER_LEFT_780_SELF_ANY=5.00 PRIMER_RIGHT_780_SELF_ANY=4.00 PRIMER_LEFT_780_SELF_END=1.00 PRIMER_RIGHT_780_SELF_END=0.00 PRIMER_LEFT_780_END_STABILITY=6.4000 PRIMER_RIGHT_780_END_STABILITY=6.4000 PRIMER_PAIR_780_COMPL_ANY=5.00 PRIMER_PAIR_780_COMPL_END=1.00 PRIMER_PAIR_780_PRODUCT_SIZE=167 PRIMER_PAIR_781_PENALTY=11.987178 PRIMER_LEFT_781_PENALTY=6.638755 PRIMER_RIGHT_781_PENALTY=5.348422 PRIMER_LEFT_781_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTGA PRIMER_RIGHT_781_SEQUENCE=GCATCACTTACAAACTCACCAACTA PRIMER_LEFT_781=411,25 PRIMER_RIGHT_781=576,25 PRIMER_LEFT_781_TM=61.639 PRIMER_RIGHT_781_TM=59.652 PRIMER_LEFT_781_GC_PERCENT=44.000 PRIMER_RIGHT_781_GC_PERCENT=40.000 PRIMER_LEFT_781_SELF_ANY=5.00 PRIMER_RIGHT_781_SELF_ANY=2.00 PRIMER_LEFT_781_SELF_END=1.00 PRIMER_RIGHT_781_SELF_END=2.00 PRIMER_LEFT_781_END_STABILITY=8.2000 PRIMER_RIGHT_781_END_STABILITY=5.7000 PRIMER_PAIR_781_COMPL_ANY=4.00 PRIMER_PAIR_781_COMPL_END=0.00 PRIMER_PAIR_781_PRODUCT_SIZE=166 PRIMER_PAIR_782_PENALTY=12.261023 PRIMER_LEFT_782_PENALTY=9.116934 PRIMER_RIGHT_782_PENALTY=3.144089 PRIMER_LEFT_782_SEQUENCE=CTGCTTAGTTGGTGAGTTTGTAAGTGA PRIMER_RIGHT_782_SEQUENCE=ACTTTCTTCAGCCAGTGGG PRIMER_LEFT_782=547,27 PRIMER_RIGHT_782=778,19 PRIMER_LEFT_782_TM=62.117 PRIMER_RIGHT_782_TM=57.856 PRIMER_LEFT_782_GC_PERCENT=40.741 PRIMER_RIGHT_782_GC_PERCENT=52.632 PRIMER_LEFT_782_SELF_ANY=4.00 PRIMER_RIGHT_782_SELF_ANY=5.00 PRIMER_LEFT_782_SELF_END=1.00 PRIMER_RIGHT_782_SELF_END=3.00 PRIMER_LEFT_782_END_STABILITY=6.4000 PRIMER_RIGHT_782_END_STABILITY=9.4000 PRIMER_PAIR_782_COMPL_ANY=4.00 PRIMER_PAIR_782_COMPL_END=0.00 PRIMER_PAIR_782_PRODUCT_SIZE=232 PRIMER_PAIR_783_PENALTY=12.283497 PRIMER_LEFT_783_PENALTY=6.944753 PRIMER_RIGHT_783_PENALTY=5.338743 PRIMER_LEFT_783_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_783_SEQUENCE=AACTCACCAACTAAGCAGCGGTA PRIMER_LEFT_783=413,25 PRIMER_RIGHT_783=564,23 PRIMER_LEFT_783_TM=61.945 PRIMER_RIGHT_783_TM=62.339 PRIMER_LEFT_783_GC_PERCENT=44.000 PRIMER_RIGHT_783_GC_PERCENT=47.826 PRIMER_LEFT_783_SELF_ANY=5.00 PRIMER_RIGHT_783_SELF_ANY=3.00 PRIMER_LEFT_783_SELF_END=0.00 PRIMER_RIGHT_783_SELF_END=2.00 PRIMER_LEFT_783_END_STABILITY=6.7000 PRIMER_RIGHT_783_END_STABILITY=8.9000 PRIMER_PAIR_783_COMPL_ANY=5.00 PRIMER_PAIR_783_COMPL_END=2.00 PRIMER_PAIR_783_PRODUCT_SIZE=152 PRIMER_PAIR_784_PENALTY=12.308424 PRIMER_LEFT_784_PENALTY=6.944753 PRIMER_RIGHT_784_PENALTY=5.363671 PRIMER_LEFT_784_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_784_SEQUENCE=AGAAGGGCATCACTTACAAACTCA PRIMER_LEFT_784=412,25 PRIMER_RIGHT_784=582,24 PRIMER_LEFT_784_TM=61.945 PRIMER_RIGHT_784_TM=61.364 PRIMER_LEFT_784_GC_PERCENT=44.000 PRIMER_RIGHT_784_GC_PERCENT=41.667 PRIMER_LEFT_784_SELF_ANY=5.00 PRIMER_RIGHT_784_SELF_ANY=4.00 PRIMER_LEFT_784_SELF_END=0.00 PRIMER_RIGHT_784_SELF_END=1.00 PRIMER_LEFT_784_END_STABILITY=7.0000 PRIMER_RIGHT_784_END_STABILITY=6.4000 PRIMER_PAIR_784_COMPL_ANY=5.00 PRIMER_PAIR_784_COMPL_END=2.00 PRIMER_PAIR_784_PRODUCT_SIZE=171 PRIMER_PAIR_785_PENALTY=12.801856 PRIMER_LEFT_785_PENALTY=7.438185 PRIMER_RIGHT_785_PENALTY=5.363671 PRIMER_LEFT_785_SEQUENCE=CAGTATTGCCAAGAAGTCTACCCTG PRIMER_RIGHT_785_SEQUENCE=TCTCCTGGTGTAAGAATTTGCACT PRIMER_LEFT_785=410,25 PRIMER_RIGHT_785=617,24 PRIMER_LEFT_785_TM=62.438 PRIMER_RIGHT_785_TM=61.364 PRIMER_LEFT_785_GC_PERCENT=48.000 PRIMER_RIGHT_785_GC_PERCENT=41.667 PRIMER_LEFT_785_SELF_ANY=5.00 PRIMER_RIGHT_785_SELF_ANY=5.00 PRIMER_LEFT_785_SELF_END=1.00 PRIMER_RIGHT_785_SELF_END=3.00 PRIMER_LEFT_785_END_STABILITY=9.7000 PRIMER_RIGHT_785_END_STABILITY=7.9000 PRIMER_PAIR_785_COMPL_ANY=4.00 PRIMER_PAIR_785_COMPL_END=2.00 PRIMER_PAIR_785_PRODUCT_SIZE=208 PRIMER_PAIR_786_PENALTY=12.993577 PRIMER_LEFT_786_PENALTY=7.562338 PRIMER_RIGHT_786_PENALTY=5.431239 PRIMER_LEFT_786_SEQUENCE=CTGCAGTATTGCCAAGAAGTCTACC PRIMER_RIGHT_786_SEQUENCE=GTAAGAATTTGCACTTGTCAGGAAC PRIMER_LEFT_786=407,25 PRIMER_RIGHT_786=608,25 PRIMER_LEFT_786_TM=62.562 PRIMER_RIGHT_786_TM=60.431 PRIMER_LEFT_786_GC_PERCENT=48.000 PRIMER_RIGHT_786_GC_PERCENT=40.000 PRIMER_LEFT_786_SELF_ANY=6.00 PRIMER_RIGHT_786_SELF_ANY=4.00 PRIMER_LEFT_786_SELF_END=1.00 PRIMER_RIGHT_786_SELF_END=1.00 PRIMER_LEFT_786_END_STABILITY=6.9000 PRIMER_RIGHT_786_END_STABILITY=7.9000 PRIMER_PAIR_786_COMPL_ANY=5.00 PRIMER_PAIR_786_COMPL_END=1.00 PRIMER_PAIR_786_PRODUCT_SIZE=202 PRIMER_PAIR_787_PENALTY=13.169124 PRIMER_LEFT_787_PENALTY=7.562338 PRIMER_RIGHT_787_PENALTY=5.606786 PRIMER_LEFT_787_SEQUENCE=CCTGCAGTATTGCCAAGAAGTCTAC PRIMER_RIGHT_787_SEQUENCE=CCTGGTGTAAGAATTTGCACTTGT PRIMER_LEFT_787=406,25 PRIMER_RIGHT_787=614,24 PRIMER_LEFT_787_TM=62.562 PRIMER_RIGHT_787_TM=61.607 PRIMER_LEFT_787_GC_PERCENT=48.000 PRIMER_RIGHT_787_GC_PERCENT=41.667 PRIMER_LEFT_787_SELF_ANY=6.00 PRIMER_RIGHT_787_SELF_ANY=5.00 PRIMER_LEFT_787_SELF_END=3.00 PRIMER_RIGHT_787_SELF_END=2.00 PRIMER_LEFT_787_END_STABILITY=5.4000 PRIMER_RIGHT_787_END_STABILITY=6.7000 PRIMER_PAIR_787_COMPL_ANY=4.00 PRIMER_PAIR_787_COMPL_END=2.00 PRIMER_PAIR_787_PRODUCT_SIZE=209 PRIMER_PAIR_788_PENALTY=13.427244 PRIMER_LEFT_788_PENALTY=7.723082 PRIMER_RIGHT_788_PENALTY=5.704162 PRIMER_LEFT_788_SEQUENCE=ATTGCCAAGAAGTCTACCCTGAACT PRIMER_RIGHT_788_SEQUENCE=CTTACAAACTCACCAACTAAGCAGC PRIMER_LEFT_788=414,25 PRIMER_RIGHT_788=570,25 PRIMER_LEFT_788_TM=62.723 PRIMER_RIGHT_788_TM=60.704 PRIMER_LEFT_788_GC_PERCENT=44.000 PRIMER_RIGHT_788_GC_PERCENT=44.000 PRIMER_LEFT_788_SELF_ANY=5.00 PRIMER_RIGHT_788_SELF_ANY=4.00 PRIMER_LEFT_788_SELF_END=1.00 PRIMER_RIGHT_788_SELF_END=3.00 PRIMER_LEFT_788_END_STABILITY=6.4000 PRIMER_RIGHT_788_END_STABILITY=9.7000 PRIMER_PAIR_788_COMPL_ANY=3.00 PRIMER_PAIR_788_COMPL_END=1.00 PRIMER_PAIR_788_PRODUCT_SIZE=157 PRIMER_PAIR_789_PENALTY=14.268879 PRIMER_LEFT_789_PENALTY=8.555845 PRIMER_RIGHT_789_PENALTY=5.713033 PRIMER_LEFT_789_SEQUENCE=GTATTGCCAAGAAGTCTACCCTGAAC PRIMER_RIGHT_789_SEQUENCE=GAACGAGAAGGGCATCACTTACA PRIMER_LEFT_789=412,26 PRIMER_RIGHT_789=587,23 PRIMER_LEFT_789_TM=62.556 PRIMER_RIGHT_789_TM=62.713 PRIMER_LEFT_789_GC_PERCENT=46.154 PRIMER_RIGHT_789_GC_PERCENT=47.826 PRIMER_LEFT_789_SELF_ANY=5.00 PRIMER_RIGHT_789_SELF_ANY=4.00 PRIMER_LEFT_789_SELF_END=0.00 PRIMER_RIGHT_789_SELF_END=0.00 PRIMER_LEFT_789_END_STABILITY=6.7000 PRIMER_RIGHT_789_END_STABILITY=6.0000 PRIMER_PAIR_789_COMPL_ANY=5.00 PRIMER_PAIR_789_COMPL_END=1.00 PRIMER_PAIR_789_PRODUCT_SIZE=176 PRIMER_PAIR_790_PENALTY=14.372398 PRIMER_LEFT_790_PENALTY=8.647019 PRIMER_RIGHT_790_PENALTY=5.725379 PRIMER_LEFT_790_SEQUENCE=TATTGCCAAGAAGTCTACCCTGAACT PRIMER_RIGHT_790_SEQUENCE=AATTTGCACTTGTCAGGAACGAG PRIMER_LEFT_790=413,26 PRIMER_RIGHT_790=603,23 PRIMER_LEFT_790_TM=62.647 PRIMER_RIGHT_790_TM=62.725 PRIMER_LEFT_790_GC_PERCENT=42.308 PRIMER_RIGHT_790_GC_PERCENT=43.478 PRIMER_LEFT_790_SELF_ANY=5.00 PRIMER_RIGHT_790_SELF_ANY=4.00 PRIMER_LEFT_790_SELF_END=1.00 PRIMER_RIGHT_790_SELF_END=2.00 PRIMER_LEFT_790_END_STABILITY=6.4000 PRIMER_RIGHT_790_END_STABILITY=8.1000 PRIMER_PAIR_790_COMPL_ANY=5.00 PRIMER_PAIR_790_COMPL_END=2.00 PRIMER_PAIR_790_PRODUCT_SIZE=191 PRIMER_PAIR_791_PENALTY=14.372398 PRIMER_LEFT_791_PENALTY=8.647019 PRIMER_RIGHT_791_PENALTY=5.725379 PRIMER_LEFT_791_SEQUENCE=AGTATTGCCAAGAAGTCTACCCTGAA PRIMER_RIGHT_791_SEQUENCE=AGAATTTGCACTTGTCAGGAACG PRIMER_LEFT_791=411,26 PRIMER_RIGHT_791=605,23 PRIMER_LEFT_791_TM=62.647 PRIMER_RIGHT_791_TM=62.725 PRIMER_LEFT_791_GC_PERCENT=42.308 PRIMER_RIGHT_791_GC_PERCENT=43.478 PRIMER_LEFT_791_SELF_ANY=5.00 PRIMER_RIGHT_791_SELF_ANY=4.00 PRIMER_LEFT_791_SELF_END=0.00 PRIMER_RIGHT_791_SELF_END=2.00 PRIMER_LEFT_791_END_STABILITY=7.0000 PRIMER_RIGHT_791_END_STABILITY=8.4000 PRIMER_PAIR_791_COMPL_ANY=5.00 PRIMER_PAIR_791_COMPL_END=2.00 PRIMER_PAIR_791_PRODUCT_SIZE=195 PRIMER_PAIR_792_PENALTY=3.168124 PRIMER_LEFT_792_PENALTY=2.096340 PRIMER_RIGHT_792_PENALTY=1.071784 PRIMER_LEFT_792_SEQUENCE=ATCAGCTGACTCGCCTGG PRIMER_RIGHT_792_SEQUENCE=GCCACAGAACATGGCAATC PRIMER_LEFT_792=2,18 PRIMER_RIGHT_792=316,19 PRIMER_LEFT_792_TM=60.096 PRIMER_RIGHT_792_TM=60.072 PRIMER_LEFT_792_GC_PERCENT=61.111 PRIMER_RIGHT_792_GC_PERCENT=52.632 PRIMER_LEFT_792_SELF_ANY=8.00 PRIMER_RIGHT_792_SELF_ANY=4.00 PRIMER_LEFT_792_SELF_END=3.00 PRIMER_RIGHT_792_SELF_END=2.00 PRIMER_LEFT_792_END_STABILITY=9.7000 PRIMER_RIGHT_792_END_STABILITY=6.9000 PRIMER_PAIR_792_COMPL_ANY=3.00 PRIMER_PAIR_792_COMPL_END=2.00 PRIMER_PAIR_792_PRODUCT_SIZE=315 PRIMER_PAIR_793_PENALTY=4.246200 PRIMER_LEFT_793_PENALTY=2.974589 PRIMER_RIGHT_793_PENALTY=1.271611 PRIMER_LEFT_793_SEQUENCE=GGATCAGCTGACTCGCCT PRIMER_RIGHT_793_SEQUENCE=GTCTGCCACAGAACATGGC PRIMER_LEFT_793=0,18 PRIMER_RIGHT_793=320,19 PRIMER_LEFT_793_TM=59.025 PRIMER_RIGHT_793_TM=60.272 PRIMER_LEFT_793_GC_PERCENT=61.111 PRIMER_RIGHT_793_GC_PERCENT=57.895 PRIMER_LEFT_793_SELF_ANY=8.00 PRIMER_RIGHT_793_SELF_ANY=5.00 PRIMER_LEFT_793_SELF_END=3.00 PRIMER_RIGHT_793_SELF_END=2.00 PRIMER_LEFT_793_END_STABILITY=11.4000 PRIMER_RIGHT_793_END_STABILITY=9.6000 PRIMER_PAIR_793_COMPL_ANY=4.00 PRIMER_PAIR_793_COMPL_END=2.00 PRIMER_PAIR_793_PRODUCT_SIZE=321 PRIMER_PAIR_794_PENALTY=4.420324 PRIMER_LEFT_794_PENALTY=3.128205 PRIMER_RIGHT_794_PENALTY=1.292120 PRIMER_LEFT_794_SEQUENCE=GATCAGCTGACTCGCCTGG PRIMER_RIGHT_794_SEQUENCE=AGTCTGCCACAGAACATGGC PRIMER_LEFT_794=1,19 PRIMER_RIGHT_794=321,20 PRIMER_LEFT_794_TM=62.128 PRIMER_RIGHT_794_TM=61.292 PRIMER_LEFT_794_GC_PERCENT=63.158 PRIMER_RIGHT_794_GC_PERCENT=55.000 PRIMER_LEFT_794_SELF_ANY=8.00 PRIMER_RIGHT_794_SELF_ANY=5.00 PRIMER_LEFT_794_SELF_END=3.00 PRIMER_RIGHT_794_SELF_END=2.00 PRIMER_LEFT_794_END_STABILITY=9.7000 PRIMER_RIGHT_794_END_STABILITY=9.6000 PRIMER_PAIR_794_COMPL_ANY=5.00 PRIMER_PAIR_794_COMPL_END=2.00 PRIMER_PAIR_794_PRODUCT_SIZE=321 PRIMER_PAIR_795_PENALTY=4.758415 PRIMER_LEFT_795_PENALTY=2.882010 PRIMER_RIGHT_795_PENALTY=1.876405 PRIMER_LEFT_795_SEQUENCE=CTGACTCGCCTGGCTCTG PRIMER_RIGHT_795_SEQUENCE=ATGGCAATCTGGGGTTCAG PRIMER_LEFT_795=7,18 PRIMER_RIGHT_795=306,19 PRIMER_LEFT_795_TM=60.882 PRIMER_RIGHT_795_TM=60.876 PRIMER_LEFT_795_GC_PERCENT=66.667 PRIMER_RIGHT_795_GC_PERCENT=52.632 PRIMER_LEFT_795_SELF_ANY=5.00 PRIMER_RIGHT_795_SELF_ANY=4.00 PRIMER_LEFT_795_SELF_END=1.00 PRIMER_RIGHT_795_SELF_END=1.00 PRIMER_LEFT_795_END_STABILITY=6.7000 PRIMER_RIGHT_795_END_STABILITY=7.0000 PRIMER_PAIR_795_COMPL_ANY=4.00 PRIMER_PAIR_795_COMPL_END=3.00 PRIMER_PAIR_795_PRODUCT_SIZE=300 PRIMER_PAIR_796_PENALTY=5.667928 PRIMER_LEFT_796_PENALTY=3.791523 PRIMER_RIGHT_796_PENALTY=1.876405 PRIMER_LEFT_796_SEQUENCE=AGCTGACTCGCCTGGCTCT PRIMER_RIGHT_796_SEQUENCE=AGAACATGGCAATCTGGGG PRIMER_LEFT_796=5,19 PRIMER_RIGHT_796=311,19 PRIMER_LEFT_796_TM=62.792 PRIMER_RIGHT_796_TM=60.876 PRIMER_LEFT_796_GC_PERCENT=63.158 PRIMER_RIGHT_796_GC_PERCENT=52.632 PRIMER_LEFT_796_SELF_ANY=5.00 PRIMER_RIGHT_796_SELF_ANY=4.00 PRIMER_LEFT_796_SELF_END=0.00 PRIMER_RIGHT_796_SELF_END=0.00 PRIMER_LEFT_796_END_STABILITY=7.9000 PRIMER_RIGHT_796_END_STABILITY=11.2000 PRIMER_PAIR_796_COMPL_ANY=4.00 PRIMER_PAIR_796_COMPL_END=2.00 PRIMER_PAIR_796_PRODUCT_SIZE=307 PRIMER_PAIR_797_PENALTY=5.924822 PRIMER_LEFT_797_PENALTY=3.873568 PRIMER_RIGHT_797_PENALTY=2.051254 PRIMER_LEFT_797_SEQUENCE=GCTGACTCGCCTGGCTCT PRIMER_RIGHT_797_SEQUENCE=AACATGGCAATCTGGGGTTC PRIMER_LEFT_797=6,18 PRIMER_RIGHT_797=309,20 PRIMER_LEFT_797_TM=61.874 PRIMER_RIGHT_797_TM=62.051 PRIMER_LEFT_797_GC_PERCENT=66.667 PRIMER_RIGHT_797_GC_PERCENT=50.000 PRIMER_LEFT_797_SELF_ANY=5.00 PRIMER_RIGHT_797_SELF_ANY=4.00 PRIMER_LEFT_797_SELF_END=0.00 PRIMER_RIGHT_797_SELF_END=0.00 PRIMER_LEFT_797_END_STABILITY=7.9000 PRIMER_RIGHT_797_END_STABILITY=7.9000 PRIMER_PAIR_797_COMPL_ANY=3.00 PRIMER_PAIR_797_COMPL_END=0.00 PRIMER_PAIR_797_PRODUCT_SIZE=304 PRIMER_PAIR_798_PENALTY=5.924822 PRIMER_LEFT_798_PENALTY=3.873568 PRIMER_RIGHT_798_PENALTY=2.051254 PRIMER_LEFT_798_SEQUENCE=AGCTGACTCGCCTGGCTC PRIMER_RIGHT_798_SEQUENCE=GAACATGGCAATCTGGGGTT PRIMER_LEFT_798=5,18 PRIMER_RIGHT_798=310,20 PRIMER_LEFT_798_TM=61.874 PRIMER_RIGHT_798_TM=62.051 PRIMER_LEFT_798_GC_PERCENT=66.667 PRIMER_RIGHT_798_GC_PERCENT=50.000 PRIMER_LEFT_798_SELF_ANY=5.00 PRIMER_RIGHT_798_SELF_ANY=4.00 PRIMER_LEFT_798_SELF_END=1.00 PRIMER_RIGHT_798_SELF_END=1.00 PRIMER_LEFT_798_END_STABILITY=9.4000 PRIMER_RIGHT_798_END_STABILITY=9.4000 PRIMER_PAIR_798_COMPL_ANY=3.00 PRIMER_PAIR_798_COMPL_END=1.00 PRIMER_PAIR_798_PRODUCT_SIZE=306 PRIMER_PAIR_799_PENALTY=6.053251 PRIMER_LEFT_799_PENALTY=3.902978 PRIMER_RIGHT_799_PENALTY=2.150273 PRIMER_LEFT_799_SEQUENCE=CTGACTCGCCTGGCTCTGA PRIMER_RIGHT_799_SEQUENCE=ATGGCAATCTGGGGTTCA PRIMER_LEFT_799=7,19 PRIMER_RIGHT_799=306,18 PRIMER_LEFT_799_TM=62.903 PRIMER_RIGHT_799_TM=59.850 PRIMER_LEFT_799_GC_PERCENT=63.158 PRIMER_RIGHT_799_GC_PERCENT=50.000 PRIMER_LEFT_799_SELF_ANY=5.00 PRIMER_RIGHT_799_SELF_ANY=4.00 PRIMER_LEFT_799_SELF_END=3.00 PRIMER_RIGHT_799_SELF_END=1.00 PRIMER_LEFT_799_END_STABILITY=6.7000 PRIMER_RIGHT_799_END_STABILITY=6.7000 PRIMER_PAIR_799_COMPL_ANY=3.00 PRIMER_PAIR_799_COMPL_END=3.00 PRIMER_PAIR_799_PRODUCT_SIZE=300 PRIMER_PAIR_800_PENALTY=6.089775 PRIMER_LEFT_800_PENALTY=3.939502 PRIMER_RIGHT_800_PENALTY=2.150273 PRIMER_LEFT_800_SEQUENCE=TGACTCGCCTGGCTCTGA PRIMER_RIGHT_800_SEQUENCE=CATGGCAATCTGGGGTTC PRIMER_LEFT_800=8,18 PRIMER_RIGHT_800=307,18 PRIMER_LEFT_800_TM=61.940 PRIMER_RIGHT_800_TM=59.850 PRIMER_LEFT_800_GC_PERCENT=61.111 PRIMER_RIGHT_800_GC_PERCENT=55.556 PRIMER_LEFT_800_SELF_ANY=5.00 PRIMER_RIGHT_800_SELF_ANY=4.00 PRIMER_LEFT_800_SELF_END=3.00 PRIMER_RIGHT_800_SELF_END=0.00 PRIMER_LEFT_800_END_STABILITY=6.7000 PRIMER_RIGHT_800_END_STABILITY=7.9000 PRIMER_PAIR_800_COMPL_ANY=3.00 PRIMER_PAIR_800_COMPL_END=2.00 PRIMER_PAIR_800_PRODUCT_SIZE=300 PRIMER_PAIR_801_PENALTY=7.071717 PRIMER_LEFT_801_PENALTY=4.921444 PRIMER_RIGHT_801_PENALTY=2.150273 PRIMER_LEFT_801_SEQUENCE=CAGCTGACTCGCCTGGCT PRIMER_RIGHT_801_SEQUENCE=GAACATGGCAATCTGGGG PRIMER_LEFT_801=4,18 PRIMER_RIGHT_801=310,18 PRIMER_LEFT_801_TM=62.921 PRIMER_RIGHT_801_TM=59.850 PRIMER_LEFT_801_GC_PERCENT=66.667 PRIMER_RIGHT_801_GC_PERCENT=55.556 PRIMER_LEFT_801_SELF_ANY=6.00 PRIMER_RIGHT_801_SELF_ANY=4.00 PRIMER_LEFT_801_SELF_END=3.00 PRIMER_RIGHT_801_SELF_END=0.00 PRIMER_LEFT_801_END_STABILITY=9.7000 PRIMER_RIGHT_801_END_STABILITY=11.2000 PRIMER_PAIR_801_COMPL_ANY=3.00 PRIMER_PAIR_801_COMPL_END=1.00 PRIMER_PAIR_801_PRODUCT_SIZE=307 = PRIMER_MIN_THREE_PRIME_DISTANCE=-1 SEQUENCE_ID=ok_regions_test4 SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-200 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MIN_TM=0 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,, PRIMER_EXPLAIN_FLAG=1 PRIMER_LEFT_EXPLAIN=considered 2261, not in any ok left region 2260, ok 1 PRIMER_RIGHT_EXPLAIN=considered 2261, not in any ok right region 2258, ok 3 PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=10.824243 PRIMER_LEFT_0_PENALTY=7.997505 PRIMER_RIGHT_0_PENALTY=2.826738 PRIMER_LEFT_0_SEQUENCE=TAACAATTGAATGTCTGCAC PRIMER_RIGHT_0_SEQUENCE=GGGGTGACTGTTAAAAGTGC PRIMER_LEFT_0=238,20 PRIMER_RIGHT_0=437,20 PRIMER_LEFT_0_TM=52.002 PRIMER_RIGHT_0_TM=57.173 PRIMER_LEFT_0_GC_PERCENT=35.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=200 = SEQUENCE_ID=ok_regions_test5 SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-200 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MIN_TM=0 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,400,20 PRIMER_EXPLAIN_FLAG=1 PRIMER_LEFT_EXPLAIN=considered 2261, not in any ok left region 2261, ok 0 PRIMER_RIGHT_EXPLAIN=considered 2261, not in any ok right region 2261, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=ok_regions_test6 SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-200 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,238,20 PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=20 PRIMER_LEFT_EXPLAIN=considered 2261, not in any ok left region 2258, ok 3 PRIMER_RIGHT_EXPLAIN=considered 2261, not in any ok right region 2260, ok 1 PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=16.900814 PRIMER_LEFT_0_PENALTY=8.903310 PRIMER_RIGHT_0_PENALTY=7.997505 PRIMER_LEFT_0_SEQUENCE=TTCGTCTGGGGGGTGTGCAC PRIMER_RIGHT_0_SEQUENCE=GTGCAGACATTCAATTGTTA PRIMER_LEFT_0=58,20 PRIMER_RIGHT_0=257,20 PRIMER_LEFT_0_TM=68.903 PRIMER_RIGHT_0_TM=52.002 PRIMER_LEFT_0_GC_PERCENT=65.000 PRIMER_RIGHT_0_GC_PERCENT=35.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=200 = SEQUENCE_ID=ok_regions_test7 SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-200 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MIN_TM=0 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,400,20;238,20,, PRIMER_EXPLAIN_FLAG=1 PRIMER_LEFT_EXPLAIN=considered 2261, not in any ok left region 2260, ok 1 PRIMER_RIGHT_EXPLAIN=considered 2261, not in any ok right region 2258, ok 3 PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=10.824243 PRIMER_LEFT_0_PENALTY=7.997505 PRIMER_RIGHT_0_PENALTY=2.826738 PRIMER_LEFT_0_SEQUENCE=TAACAATTGAATGTCTGCAC PRIMER_RIGHT_0_SEQUENCE=GGGGTGACTGTTAAAAGTGC PRIMER_LEFT_0=238,20 PRIMER_RIGHT_0=437,20 PRIMER_LEFT_0_TM=52.002 PRIMER_RIGHT_0_TM=57.173 PRIMER_LEFT_0_GC_PERCENT=35.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=200 = SEQUENCE_ID=ok_regions_test8 SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-200 150-182 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MIN_TM=0 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,400,20 PRIMER_EXPLAIN_FLAG=1 PRIMER_LEFT_EXPLAIN=considered 2311, not in any ok left region 2310, ok 1 PRIMER_RIGHT_EXPLAIN=considered 2311, not in any ok right region 2310, ok 1 PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=13.190170 PRIMER_LEFT_0_PENALTY=7.997505 PRIMER_RIGHT_0_PENALTY=5.192665 PRIMER_LEFT_0_SEQUENCE=TAACAATTGAATGTCTGCAC PRIMER_RIGHT_0_SEQUENCE=GCATACCGCCTAAAGATAAA PRIMER_LEFT_0=238,20 PRIMER_RIGHT_0=419,20 PRIMER_LEFT_0_TM=52.002 PRIMER_RIGHT_0_TM=54.807 PRIMER_LEFT_0_GC_PERCENT=35.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=182 = primer3-2.3.6/test/primer_output0000664000077300007730000015256412231156245015713 0ustar ioanaioanaPRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 P3_FILE_FLAG=1 SEQUENCE_ID=targets_test SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 PRIMER_PAIR_1_PENALTY=4.086374 PRIMER_LEFT_1_PENALTY=1.771733 PRIMER_RIGHT_1_PENALTY=2.314641 PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_1=1,20 PRIMER_RIGHT_1=160,18 PRIMER_LEFT_1_TM=58.228 PRIMER_RIGHT_1_TM=59.685 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=10.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=12.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=160 PRIMER_PAIR_2_PENALTY=5.046339 PRIMER_LEFT_2_PENALTY=2.731698 PRIMER_RIGHT_2_PENALTY=2.314641 PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=160,18 PRIMER_LEFT_2_TM=57.268 PRIMER_RIGHT_2_TM=59.685 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY=12.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=12.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=161 = SEQUENCE_ID=SP01-02.txt SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.334195 PRIMER_LEFT_0_PENALTY=2.101896 PRIMER_RIGHT_0_PENALTY=0.232299 PRIMER_LEFT_0_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_0_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_LEFT_0=9,22 PRIMER_RIGHT_0=247,20 PRIMER_LEFT_0_TM=59.898 PRIMER_RIGHT_0_TM=60.232 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=239 PRIMER_PAIR_1_PENALTY=2.773493 PRIMER_LEFT_1_PENALTY=2.101896 PRIMER_RIGHT_1_PENALTY=0.671597 PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT PRIMER_LEFT_1=9,22 PRIMER_RIGHT_1=246,20 PRIMER_LEFT_1_TM=59.898 PRIMER_RIGHT_1_TM=59.328 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=238 PRIMER_PAIR_2_PENALTY=2.846821 PRIMER_LEFT_2_PENALTY=2.101896 PRIMER_RIGHT_2_PENALTY=0.744925 PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA PRIMER_LEFT_2=9,22 PRIMER_RIGHT_2=248,20 PRIMER_LEFT_2_TM=59.898 PRIMER_RIGHT_2_TM=60.745 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=240 PRIMER_PAIR_3_PENALTY=3.098430 PRIMER_LEFT_3_PENALTY=2.866131 PRIMER_RIGHT_3_PENALTY=0.232299 PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_LEFT_3=11,21 PRIMER_RIGHT_3=247,20 PRIMER_LEFT_3_TM=58.134 PRIMER_RIGHT_3_TM=60.232 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=237 PRIMER_PAIR_4_PENALTY=3.235559 PRIMER_LEFT_4_PENALTY=3.003259 PRIMER_RIGHT_4_PENALTY=0.232299 PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_LEFT_4=10,22 PRIMER_RIGHT_4=247,20 PRIMER_LEFT_4_TM=58.997 PRIMER_RIGHT_4_TM=60.232 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=238 = SEQUENCE_ID=SP01-03.txt SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.785028 PRIMER_LEFT_0_PENALTY=0.040772 PRIMER_RIGHT_0_PENALTY=0.744257 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_RIGHT_0_SEQUENCE=ATCCTGCTGAAGCAAATGGA PRIMER_LEFT_0=72,20 PRIMER_RIGHT_0=173,20 PRIMER_LEFT_0_TM=59.959 PRIMER_RIGHT_0_TM=60.744 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=102 PRIMER_PAIR_1_PENALTY=0.785028 PRIMER_LEFT_1_PENALTY=0.040772 PRIMER_RIGHT_1_PENALTY=0.744257 PRIMER_LEFT_1_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_RIGHT_1_SEQUENCE=GATCCTGCTGAAGCAAATGG PRIMER_LEFT_1=72,20 PRIMER_RIGHT_1=174,20 PRIMER_LEFT_1_TM=59.959 PRIMER_RIGHT_1_TM=60.744 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=103 PRIMER_PAIR_2_PENALTY=1.015937 PRIMER_LEFT_2_PENALTY=0.271680 PRIMER_RIGHT_2_PENALTY=0.744257 PRIMER_LEFT_2_SEQUENCE=CTGCAGGTTTAGAAGGGGTG PRIMER_RIGHT_2_SEQUENCE=ATCCTGCTGAAGCAAATGGA PRIMER_LEFT_2=64,20 PRIMER_RIGHT_2=173,20 PRIMER_LEFT_2_TM=59.728 PRIMER_RIGHT_2_TM=60.744 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=8.1000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=110 PRIMER_PAIR_3_PENALTY=1.015937 PRIMER_LEFT_3_PENALTY=0.271680 PRIMER_RIGHT_3_PENALTY=0.744257 PRIMER_LEFT_3_SEQUENCE=CTGCAGGTTTAGAAGGGGTG PRIMER_RIGHT_3_SEQUENCE=GATCCTGCTGAAGCAAATGG PRIMER_LEFT_3=64,20 PRIMER_RIGHT_3=174,20 PRIMER_LEFT_3_TM=59.728 PRIMER_RIGHT_3_TM=60.744 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=8.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=111 PRIMER_PAIR_4_PENALTY=1.015937 PRIMER_LEFT_4_PENALTY=0.271680 PRIMER_RIGHT_4_PENALTY=0.744257 PRIMER_LEFT_4_SEQUENCE=CACCCTGCAGGTTTAGAAGG PRIMER_RIGHT_4_SEQUENCE=ATCCTGCTGAAGCAAATGGA PRIMER_LEFT_4=60,20 PRIMER_RIGHT_4=173,20 PRIMER_LEFT_4_TM=59.728 PRIMER_RIGHT_4_TM=60.744 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=114 = SEQUENCE_ID=SP01-06.txt SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.738411 PRIMER_LEFT_0_PENALTY=4.596160 PRIMER_RIGHT_0_PENALTY=2.142251 PRIMER_LEFT_0_SEQUENCE=TATGAATCGCAATCAAATTTTTTC PRIMER_RIGHT_0_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_LEFT_0=30,24 PRIMER_RIGHT_0=129,18 PRIMER_LEFT_0_TM=59.404 PRIMER_RIGHT_0_TM=60.142 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=7.148392 PRIMER_LEFT_1_PENALTY=5.006141 PRIMER_RIGHT_1_PENALTY=2.142251 PRIMER_LEFT_1_SEQUENCE=TTATGAATCGCAATCAAATTTTTT PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_LEFT_1=29,24 PRIMER_RIGHT_1=129,18 PRIMER_LEFT_1_TM=58.994 PRIMER_RIGHT_1_TM=60.142 PRIMER_LEFT_1_GC_PERCENT=20.833 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=101 PRIMER_PAIR_2_PENALTY=7.344055 PRIMER_LEFT_2_PENALTY=5.201804 PRIMER_RIGHT_2_PENALTY=2.142251 PRIMER_LEFT_2_SEQUENCE=TATGAATCGCAATCAAATTTTTTCT PRIMER_RIGHT_2_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_LEFT_2=30,25 PRIMER_RIGHT_2=129,18 PRIMER_LEFT_2_TM=60.202 PRIMER_RIGHT_2_TM=60.142 PRIMER_LEFT_2_GC_PERCENT=24.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=7.346874 PRIMER_LEFT_3_PENALTY=5.204623 PRIMER_RIGHT_3_PENALTY=2.142251 PRIMER_LEFT_3_SEQUENCE=TATGAATCGCAATCAAATTTTTT PRIMER_RIGHT_3_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_LEFT_3=30,23 PRIMER_RIGHT_3=129,18 PRIMER_LEFT_3_TM=57.795 PRIMER_RIGHT_3_TM=60.142 PRIMER_LEFT_3_GC_PERCENT=21.739 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=7.346874 PRIMER_LEFT_4_PENALTY=5.204623 PRIMER_RIGHT_4_PENALTY=2.142251 PRIMER_LEFT_4_SEQUENCE=TTATGAATCGCAATCAAATTTTT PRIMER_RIGHT_4_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_LEFT_4=29,23 PRIMER_RIGHT_4=129,18 PRIMER_LEFT_4_TM=57.795 PRIMER_RIGHT_4_TM=60.142 PRIMER_LEFT_4_GC_PERCENT=21.739 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.6000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=101 = SEQUENCE_ID=SP01-15.txt SEQUENCE_TEMPLATE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=SP01-19.txt SEQUENCE_TEMPLATE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.269854 PRIMER_LEFT_0_PENALTY=0.072951 PRIMER_RIGHT_0_PENALTY=0.196903 PRIMER_LEFT_0_SEQUENCE=TATTCCTGTCCCTGTGCCTC PRIMER_RIGHT_0_SEQUENCE=CCCTCATCCAGTGCTCTTTC PRIMER_LEFT_0=48,20 PRIMER_RIGHT_0=183,20 PRIMER_LEFT_0_TM=60.073 PRIMER_RIGHT_0_TM=59.803 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=136 PRIMER_PAIR_1_PENALTY=0.269854 PRIMER_LEFT_1_PENALTY=0.072951 PRIMER_RIGHT_1_PENALTY=0.196903 PRIMER_LEFT_1_SEQUENCE=TATTCCTGTCCCTGTGCCTC PRIMER_RIGHT_1_SEQUENCE=TCCCTCATCCAGTGCTCTTT PRIMER_LEFT_1=48,20 PRIMER_RIGHT_1=184,20 PRIMER_LEFT_1_TM=60.073 PRIMER_RIGHT_1_TM=59.803 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=137 PRIMER_PAIR_2_PENALTY=0.303362 PRIMER_LEFT_2_PENALTY=0.072951 PRIMER_RIGHT_2_PENALTY=0.230410 PRIMER_LEFT_2_SEQUENCE=TATTCCTGTCCCTGTGCCTC PRIMER_RIGHT_2_SEQUENCE=CAGTGCTCTTTCTGCCAGTG PRIMER_LEFT_2=48,20 PRIMER_RIGHT_2=175,20 PRIMER_LEFT_2_TM=60.073 PRIMER_RIGHT_2_TM=59.770 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=128 PRIMER_PAIR_3_PENALTY=0.593847 PRIMER_LEFT_3_PENALTY=0.396944 PRIMER_RIGHT_3_PENALTY=0.196903 PRIMER_LEFT_3_SEQUENCE=GCCTCAGAAACCTGGCTACA PRIMER_RIGHT_3_SEQUENCE=CCCTCATCCAGTGCTCTTTC PRIMER_LEFT_3=63,20 PRIMER_RIGHT_3=183,20 PRIMER_LEFT_3_TM=60.397 PRIMER_RIGHT_3_TM=59.803 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=5.7000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=121 PRIMER_PAIR_4_PENALTY=0.593847 PRIMER_LEFT_4_PENALTY=0.396944 PRIMER_RIGHT_4_PENALTY=0.196903 PRIMER_LEFT_4_SEQUENCE=GCCTCAGAAACCTGGCTACA PRIMER_RIGHT_4_SEQUENCE=TCCCTCATCCAGTGCTCTTT PRIMER_LEFT_4=63,20 PRIMER_RIGHT_4=184,20 PRIMER_LEFT_4_TM=60.397 PRIMER_RIGHT_4_TM=59.803 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=5.7000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=122 = SEQUENCE_ID=SP01-20.txt SEQUENCE_TEMPLATE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=SP01-21.txt SEQUENCE_TEMPLATE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.103491 PRIMER_LEFT_0_PENALTY=2.224770 PRIMER_RIGHT_0_PENALTY=2.878720 PRIMER_LEFT_0_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_0_SEQUENCE=CACACACACAACACACAGTCAA PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=155,22 PRIMER_LEFT_0_TM=57.775 PRIMER_RIGHT_0_TM=59.121 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=6.00 PRIMER_PAIR_0_PRODUCT_SIZE=156 PRIMER_PAIR_1_PENALTY=5.103491 PRIMER_LEFT_1_PENALTY=2.224770 PRIMER_RIGHT_1_PENALTY=2.878720 PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_1_SEQUENCE=CAACAGACACACACACACAACA PRIMER_LEFT_1=0,20 PRIMER_RIGHT_1=164,22 PRIMER_LEFT_1_TM=57.775 PRIMER_RIGHT_1_TM=59.121 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=165 PRIMER_PAIR_2_PENALTY=5.103491 PRIMER_LEFT_2_PENALTY=2.224770 PRIMER_RIGHT_2_PENALTY=2.878720 PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_2_SEQUENCE=CACAACAGACACACACACACAA PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=166,22 PRIMER_LEFT_2_TM=57.775 PRIMER_RIGHT_2_TM=59.121 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=8.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=167 PRIMER_PAIR_3_PENALTY=5.288748 PRIMER_LEFT_3_PENALTY=2.224770 PRIMER_RIGHT_3_PENALTY=3.063978 PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_3_SEQUENCE=ACACACACACAACACACAGTCAA PRIMER_LEFT_3=0,20 PRIMER_RIGHT_3=156,23 PRIMER_LEFT_3_TM=57.775 PRIMER_RIGHT_3_TM=60.064 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=6.00 PRIMER_PAIR_3_PRODUCT_SIZE=157 PRIMER_PAIR_4_PENALTY=5.288748 PRIMER_LEFT_4_PENALTY=2.224770 PRIMER_RIGHT_4_PENALTY=3.063978 PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_4_SEQUENCE=AACAGACACACACACACAACACA PRIMER_LEFT_4=0,20 PRIMER_RIGHT_4=163,23 PRIMER_LEFT_4_TM=57.775 PRIMER_RIGHT_4_TM=60.064 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=7.00 PRIMER_PAIR_4_PRODUCT_SIZE=164 = SEQUENCE_ID=SP03-40.txt SEQUENCE_TEMPLATE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=SP04-07.txt SEQUENCE_TEMPLATE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.103348 PRIMER_LEFT_0_PENALTY=0.051674 PRIMER_RIGHT_0_PENALTY=0.051674 PRIMER_LEFT_0_SEQUENCE=CCCTACATACAGCCAAGGGA PRIMER_RIGHT_0_SEQUENCE=TTCTTTCCGTGGCTCTCCTA PRIMER_LEFT_0=21,20 PRIMER_RIGHT_0=281,20 PRIMER_LEFT_0_TM=59.948 PRIMER_RIGHT_0_TM=59.948 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=261 PRIMER_PAIR_1_PENALTY=0.103348 PRIMER_LEFT_1_PENALTY=0.051674 PRIMER_RIGHT_1_PENALTY=0.051674 PRIMER_LEFT_1_SEQUENCE=TCCCTACATACAGCCAAGGG PRIMER_RIGHT_1_SEQUENCE=TTCTTTCCGTGGCTCTCCTA PRIMER_LEFT_1=20,20 PRIMER_RIGHT_1=281,20 PRIMER_LEFT_1_TM=59.948 PRIMER_RIGHT_1_TM=59.948 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=7.2000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=262 PRIMER_PAIR_2_PENALTY=0.117997 PRIMER_LEFT_2_PENALTY=0.066323 PRIMER_RIGHT_2_PENALTY=0.051674 PRIMER_LEFT_2_SEQUENCE=CTAAGAATGTGGCCAAGGGA PRIMER_RIGHT_2_SEQUENCE=TTCTTTCCGTGGCTCTCCTA PRIMER_LEFT_2=122,20 PRIMER_RIGHT_2=281,20 PRIMER_LEFT_2_TM=60.066 PRIMER_RIGHT_2_TM=59.948 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=7.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=160 PRIMER_PAIR_3_PENALTY=0.117997 PRIMER_LEFT_3_PENALTY=0.066323 PRIMER_RIGHT_3_PENALTY=0.051674 PRIMER_LEFT_3_SEQUENCE=TCCCTAAGAATGTGGCCAAG PRIMER_RIGHT_3_SEQUENCE=TTCTTTCCGTGGCTCTCCTA PRIMER_LEFT_3=119,20 PRIMER_RIGHT_3=281,20 PRIMER_LEFT_3_TM=60.066 PRIMER_RIGHT_3_TM=59.948 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=163 PRIMER_PAIR_4_PENALTY=0.117997 PRIMER_LEFT_4_PENALTY=0.051674 PRIMER_RIGHT_4_PENALTY=0.066323 PRIMER_LEFT_4_SEQUENCE=CCCTACATACAGCCAAGGGA PRIMER_RIGHT_4_SEQUENCE=CTTGGCCACATTCTTAGGGA PRIMER_LEFT_4=21,20 PRIMER_RIGHT_4=138,20 PRIMER_LEFT_4_TM=59.948 PRIMER_RIGHT_4_TM=60.066 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=118 = SEQUENCE_ID=AGAG SEQUENCE_TEMPLATE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=long_seq SEQUENCE_TEMPLATE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.361544 PRIMER_LEFT_0_PENALTY=0.221791 PRIMER_RIGHT_0_PENALTY=0.139753 PRIMER_LEFT_0_SEQUENCE=gatcgatgcatgcatgctac PRIMER_RIGHT_0_SEQUENCE=cccagctagctagcatcgtc PRIMER_LEFT_0=445,20 PRIMER_RIGHT_0=676,20 PRIMER_LEFT_0_TM=60.222 PRIMER_RIGHT_0_TM=60.140 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=10.00 PRIMER_RIGHT_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=8.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=232 PRIMER_PAIR_1_PENALTY=0.398026 PRIMER_LEFT_1_PENALTY=0.258273 PRIMER_RIGHT_1_PENALTY=0.139753 PRIMER_LEFT_1_SEQUENCE=gatgctagctaggcgatgct PRIMER_RIGHT_1_SEQUENCE=cccagctagctagcatcgtc PRIMER_LEFT_1=408,20 PRIMER_RIGHT_1=676,20 PRIMER_LEFT_1_TM=59.742 PRIMER_RIGHT_1_TM=60.140 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.1000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=12.00 PRIMER_PAIR_1_COMPL_END=7.00 PRIMER_PAIR_1_PRODUCT_SIZE=269 PRIMER_PAIR_2_PENALTY=0.405097 PRIMER_LEFT_2_PENALTY=0.120169 PRIMER_RIGHT_2_PENALTY=0.284927 PRIMER_LEFT_2_SEQUENCE=gcgatcgatgctagctagct PRIMER_RIGHT_2_SEQUENCE=ctagctagatcgccgctacg PRIMER_LEFT_2=372,20 PRIMER_RIGHT_2=595,20 PRIMER_LEFT_2_TM=59.880 PRIMER_RIGHT_2_TM=60.285 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=10.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=8.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=7.4000 PRIMER_PAIR_2_COMPL_ANY=10.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=224 PRIMER_PAIR_3_PENALTY=0.500121 PRIMER_LEFT_3_PENALTY=0.221791 PRIMER_RIGHT_3_PENALTY=0.278329 PRIMER_LEFT_3_SEQUENCE=gatcgatgcatgcatgctac PRIMER_RIGHT_3_SEQUENCE=gtcagctagctagatcgccg PRIMER_LEFT_3=445,20 PRIMER_RIGHT_3=600,20 PRIMER_LEFT_3_TM=60.222 PRIMER_RIGHT_3_TM=60.278 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=10.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=8.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=13.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_4_PENALTY=0.506718 PRIMER_LEFT_4_PENALTY=0.221791 PRIMER_RIGHT_4_PENALTY=0.284927 PRIMER_LEFT_4_SEQUENCE=gatcgatgcatgcatgctac PRIMER_RIGHT_4_SEQUENCE=ctagctagatcgccgctacg PRIMER_LEFT_4=445,20 PRIMER_RIGHT_4=595,20 PRIMER_LEFT_4_TM=60.222 PRIMER_RIGHT_4_TM=60.285 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=10.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=8.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=7.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=151 = SEQUENCE_ID=bad_letter SEQUENCE_TEMPLATE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC PRIMER_ERROR=Unrecognized base in input sequence = SEQUENCE_ID=bad_target SEQUENCE_TEMPLATE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC SEQUENCE_TARGET=50,10 SEQUENCE_TARGET=500,50 PRIMER_ERROR=TARGET beyond end of sequence = SEQUENCE_ID=other_bad_target SEQUENCE_TEMPLATE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC SEQUENCE_TARGET=100,1000 PRIMER_ERROR=TARGET beyond end of sequence = SEQUENCE_ID=included_test SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=20,228 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.103491 PRIMER_LEFT_0_PENALTY=2.224770 PRIMER_RIGHT_0_PENALTY=2.878720 PRIMER_LEFT_0_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_0_SEQUENCE=CACACACACAACACACAGTCAA PRIMER_LEFT_0=20,20 PRIMER_RIGHT_0=175,22 PRIMER_LEFT_0_TM=57.775 PRIMER_RIGHT_0_TM=59.121 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=6.00 PRIMER_PAIR_0_PRODUCT_SIZE=156 PRIMER_PAIR_1_PENALTY=5.103491 PRIMER_LEFT_1_PENALTY=2.224770 PRIMER_RIGHT_1_PENALTY=2.878720 PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_1_SEQUENCE=CAACAGACACACACACACAACA PRIMER_LEFT_1=20,20 PRIMER_RIGHT_1=184,22 PRIMER_LEFT_1_TM=57.775 PRIMER_RIGHT_1_TM=59.121 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=165 PRIMER_PAIR_2_PENALTY=5.103491 PRIMER_LEFT_2_PENALTY=2.224770 PRIMER_RIGHT_2_PENALTY=2.878720 PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_2_SEQUENCE=CACAACAGACACACACACACAA PRIMER_LEFT_2=20,20 PRIMER_RIGHT_2=186,22 PRIMER_LEFT_2_TM=57.775 PRIMER_RIGHT_2_TM=59.121 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=8.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=167 PRIMER_PAIR_3_PENALTY=5.288748 PRIMER_LEFT_3_PENALTY=2.224770 PRIMER_RIGHT_3_PENALTY=3.063978 PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_3_SEQUENCE=ACACACACACAACACACAGTCAA PRIMER_LEFT_3=20,20 PRIMER_RIGHT_3=176,23 PRIMER_LEFT_3_TM=57.775 PRIMER_RIGHT_3_TM=60.064 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=6.00 PRIMER_PAIR_3_PRODUCT_SIZE=157 PRIMER_PAIR_4_PENALTY=5.288748 PRIMER_LEFT_4_PENALTY=2.224770 PRIMER_RIGHT_4_PENALTY=3.063978 PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_4_SEQUENCE=AACAGACACACACACACAACACA PRIMER_LEFT_4=20,20 PRIMER_RIGHT_4=183,23 PRIMER_LEFT_4_TM=57.775 PRIMER_RIGHT_4_TM=60.064 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=7.00 PRIMER_PAIR_4_PRODUCT_SIZE=164 = SEQUENCE_ID=included_test1 SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=20-200 PRIMER_ERROR=Illegal SEQUENCE_INCLUDED_REGION value: 20-200 = PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MAX_SELF_END=8 SEQUENCE_ID=included_test_w_middle SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=20,228 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.103491 PRIMER_LEFT_0_PENALTY=2.224770 PRIMER_RIGHT_0_PENALTY=2.878720 PRIMER_INTERNAL_0_PENALTY=1.680010 PRIMER_LEFT_0_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_0_SEQUENCE=CACACACACAACACACAGTCAA PRIMER_INTERNAL_0_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG PRIMER_LEFT_0=20,20 PRIMER_RIGHT_0=175,22 PRIMER_INTERNAL_0=78,21 PRIMER_LEFT_0_TM=57.775 PRIMER_RIGHT_0_TM=59.121 PRIMER_INTERNAL_0_TM=60.680 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_INTERNAL_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=1.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=6.00 PRIMER_PAIR_0_PRODUCT_SIZE=156 PRIMER_PAIR_1_PENALTY=5.103491 PRIMER_LEFT_1_PENALTY=2.224770 PRIMER_RIGHT_1_PENALTY=2.878720 PRIMER_INTERNAL_1_PENALTY=1.680010 PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_1_SEQUENCE=CAACAGACACACACACACAACA PRIMER_INTERNAL_1_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG PRIMER_LEFT_1=20,20 PRIMER_RIGHT_1=184,22 PRIMER_INTERNAL_1=78,21 PRIMER_LEFT_1_TM=57.775 PRIMER_RIGHT_1_TM=59.121 PRIMER_INTERNAL_1_TM=60.680 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_INTERNAL_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_INTERNAL_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=165 PRIMER_PAIR_2_PENALTY=5.103491 PRIMER_LEFT_2_PENALTY=2.224770 PRIMER_RIGHT_2_PENALTY=2.878720 PRIMER_INTERNAL_2_PENALTY=1.680010 PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_2_SEQUENCE=CACAACAGACACACACACACAA PRIMER_INTERNAL_2_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG PRIMER_LEFT_2=20,20 PRIMER_RIGHT_2=186,22 PRIMER_INTERNAL_2=78,21 PRIMER_LEFT_2_TM=57.775 PRIMER_RIGHT_2_TM=59.121 PRIMER_INTERNAL_2_TM=60.680 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_INTERNAL_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_INTERNAL_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=8.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=167 PRIMER_PAIR_3_PENALTY=5.288748 PRIMER_LEFT_3_PENALTY=2.224770 PRIMER_RIGHT_3_PENALTY=3.063978 PRIMER_INTERNAL_3_PENALTY=1.680010 PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_3_SEQUENCE=ACACACACACAACACACAGTCAA PRIMER_INTERNAL_3_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG PRIMER_LEFT_3=20,20 PRIMER_RIGHT_3=176,23 PRIMER_INTERNAL_3=78,21 PRIMER_LEFT_3_TM=57.775 PRIMER_RIGHT_3_TM=60.064 PRIMER_INTERNAL_3_TM=60.680 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_INTERNAL_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=1.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=6.00 PRIMER_PAIR_3_PRODUCT_SIZE=157 PRIMER_PAIR_4_PENALTY=5.288748 PRIMER_LEFT_4_PENALTY=2.224770 PRIMER_RIGHT_4_PENALTY=3.063978 PRIMER_INTERNAL_4_PENALTY=1.680010 PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_4_SEQUENCE=AACAGACACACACACACAACACA PRIMER_INTERNAL_4_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG PRIMER_LEFT_4=20,20 PRIMER_RIGHT_4=183,23 PRIMER_INTERNAL_4=78,21 PRIMER_LEFT_4_TM=57.775 PRIMER_RIGHT_4_TM=60.064 PRIMER_INTERNAL_4_TM=60.680 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_INTERNAL_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_INTERNAL_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=7.00 PRIMER_PAIR_4_PRODUCT_SIZE=164 = SEQUENCE_ID=long_primer_lists PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 P3_FILE_FLAG=0 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=20 PRIMER_PAIR_MAX_COMPL_ANY=20 PRIMER_PAIR_MAX_COMPL_END=20 SEQUENCE_TEMPLATE=TTTTTTTTATTATTATACTTCAAGTTTTAGGGTACATGTGCACAACGGCAGGTTTGTTATATATGTATACATGTGCCATGTTGGTGTGCTGCACCCATTAACTCGTCATTTAGCATTAGGTATATCTCCTAGTGCTATCCCTGCCCCTGCCCCCCACCCCATAACAGTCCCCAGAGTGTGATGTTCCTCTTCATGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGTGAGAACATGCGGTGTTTGGTTTATTGTCCTTGTGATAGTTTGCTGAGAATGATAGTTTCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAATCTATCGTTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATTGTGAATAGTGCTGCAATAAACATACGTGTGCATGTGTCTTTATAGCAGCATGATTTATAATCCTTTGGGTATCTACCCAGTAATGGGATGGCTGGGTCAAATGGTATTTCTAGTTCTAGATTCCTGAGGAATTGCCACACTGACTTCCGCAATGGTTGAACTAGTTAATTAGATCCCGTTTGTCC PRIMER_LEFT_EXPLAIN=considered 5210, GC content failed 120, ok 5090 PRIMER_RIGHT_EXPLAIN=considered 5210, GC content failed 24, ok 5186 PRIMER_INTERNAL_EXPLAIN=considered 5985, GC content failed 120, low tm 2448, high tm 1678, ok 1739 PRIMER_PAIR_EXPLAIN=considered 7, unacceptable product size 6, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.316568 PRIMER_LEFT_0_PENALTY=0.278124 PRIMER_RIGHT_0_PENALTY=0.038444 PRIMER_INTERNAL_0_PENALTY=0.609876 PRIMER_LEFT_0_SEQUENCE=TGTGCTGCACCCATTAACTC PRIMER_RIGHT_0_SEQUENCE=AGGAACATCACACTCTGGGG PRIMER_INTERNAL_0_SEQUENCE=CTAGTGCTATCCCTGCCCCT PRIMER_LEFT_0=84,20 PRIMER_RIGHT_0=187,20 PRIMER_INTERNAL_0=128,20 PRIMER_LEFT_0_TM=59.722 PRIMER_RIGHT_0_TM=59.962 PRIMER_INTERNAL_0_TM=60.610 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=11.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=104 = SEQUENCE_ID=end_complementarity_failure PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=2 PRIMER_PAIR_MAX_COMPL_ANY=20 PRIMER_PAIR_MAX_COMPL_END=2 PRIMER_INTERNAL_MAX_SELF_END=2 SEQUENCE_TEMPLATE=TTTTTTTTATTATTATACTTCAAGTTTTAGGGTACATGTGCACAACGGCAGGTTTGTTATATATGTATACATGTGCCATGTTGGTGTGCTGCACCCATTAACTCGTCATTTAGCATTAGGTATATCTCCTAGTGCTATCCCTGCCCCTGCCCCCCACCCCATAACAGTCCCCAGAGTGTGATGTTCCTCTTCATGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGTGAGAACATGCGGTGTTTGGTTTATTGTCCTTGTGATAGTTTGCTGAGAATGATAGTTTCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCC PRIMER_LEFT_EXPLAIN=considered 2740, GC content failed 120, ok 2620 PRIMER_RIGHT_EXPLAIN=considered 2740, GC content failed 24, ok 2716 PRIMER_INTERNAL_EXPLAIN=considered 3515, GC content failed 120, low tm 1357, high tm 1080, high end compl 1, ok 957 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.316568 PRIMER_LEFT_0_PENALTY=0.278124 PRIMER_RIGHT_0_PENALTY=0.038444 PRIMER_INTERNAL_0_PENALTY=0.609876 PRIMER_LEFT_0_SEQUENCE=TGTGCTGCACCCATTAACTC PRIMER_RIGHT_0_SEQUENCE=AGGAACATCACACTCTGGGG PRIMER_INTERNAL_0_SEQUENCE=CTAGTGCTATCCCTGCCCCT PRIMER_LEFT_0=84,20 PRIMER_RIGHT_0=187,20 PRIMER_INTERNAL_0=128,20 PRIMER_LEFT_0_TM=59.722 PRIMER_RIGHT_0_TM=59.962 PRIMER_INTERNAL_0_TM=60.610 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=11.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=104 = SEQUENCE_TEMPLATE=GATCGATCGATGCTAGCTGACTGATCGTAGCTAGTCGATCGTAGCTAGCTATCGTACTACTATCATCTAGCTACTGATCGATGGGGCTGTGCTTCGATGCGGTGACGGCTAGCTAGCTGATCGGCTAGCTGACGCTGATCGATGCTATACTACATCTACTACTACGATCACTGGGCATCGTAGCTAGCTACACATCTAGCTGACAGC P3_FILE_FLAG=1 PRIMER_ERROR=Need PRIMER_SEQUENCE_ID if PRIMER_FILE_FLAG is not 0 = SEQUENCE_ID=bad_included_region SEQUENCE_TEMPLATE=ATCTGCTAGCTGATCGAGCTATCATGCTAGCTGATCATGCTAGCTATCGTACTACTATCATCTACTGATCATGCTAGCTACTATCATCTACTATCATCATCTAGCTACTAGCTACTACGATCTACTACTATCATCTACTAGCTACTATCACTACTACTATCATCATCTACTACTACATCACACTGATCGTGGGCTAGCTGGCAGCGCG SEQUENCE_INCLUDED_REGION=-1,100 P3_FILE_FLAG=0 PRIMER_ERROR=Illegal value for SEQUENCE_INCLUDED_REGION = SEQUENCE_ID=bad_included_region1 SEQUENCE_TEMPLATE=GTAGCAGCTAGCTAGCTAGTCGATCGTAGCTAGCTAGTCATCTACGTAGCTAGCTAGTCATCGTAGCTGATCGATGCTAGCTAGCTGATCGGGCGACTGTTCATATGATGGGCTACTGACTGATCGTAGCTGGCGATCGTAGCGAGGCGATCTAGCTAGCTAGCTATCGGCTACTGATCGTACTGACTGATGCTAGCTAGGGCGTAGCTAG SEQUENCE_INCLUDED_REGION=150,-90 PRIMER_ERROR=Illegal value for SEQUENCE_INCLUDED_REGION = SEQUENCE_ID=SP01-06-with-repeat1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MISPRIMING_LIBRARY=../not_for_production.lib PRIMER_MAX_LIBRARY_MISPRIMING=14 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_EXPLAIN=considered 136, too many Ns 36, GC content failed 1, high end compl 48, ok 51 PRIMER_RIGHT_EXPLAIN=considered 370, high end compl 191, ok 179 PRIMER_INTERNAL_EXPLAIN=considered 911, too many Ns 36, GC content failed 5, low tm 420, high tm 182, high end compl 110, ok 158 PRIMER_PAIR_EXPLAIN=considered 333, unacceptable product size 332, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=6.738411 PRIMER_LEFT_0_PENALTY=4.596160 PRIMER_RIGHT_0_PENALTY=2.142251 PRIMER_INTERNAL_0_PENALTY=0.727969 PRIMER_LEFT_0_SEQUENCE=TATGAATCGCAATCAAATTTTTTC PRIMER_RIGHT_0_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_INTERNAL_0_SEQUENCE=TGACCTGATAGAAACGCCAA PRIMER_LEFT_0=30,24 PRIMER_RIGHT_0=129,18 PRIMER_INTERNAL_0=56,20 PRIMER_LEFT_0_TM=59.404 PRIMER_RIGHT_0_TM=60.142 PRIMER_INTERNAL_0_TM=59.272 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 = SEQUENCE_ID=SP01-06-with-repeat2 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MISPRIMING_LIBRARY=../not_for_production.lib PRIMER_MAX_LIBRARY_MISPRIMING=14 PRIMER_INTERNAL_MISHYB_LIBRARY=../not_for_production.lib PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=14 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_EXPLAIN=considered 136, too many Ns 36, GC content failed 1, high end compl 48, ok 51 PRIMER_RIGHT_EXPLAIN=considered 370, high end compl 191, ok 179 PRIMER_INTERNAL_EXPLAIN=considered 911, too many Ns 36, GC content failed 5, low tm 420, high tm 182, high end compl 110, ok 158 PRIMER_PAIR_EXPLAIN=considered 333, unacceptable product size 332, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=6.738411 PRIMER_LEFT_0_PENALTY=4.596160 PRIMER_RIGHT_0_PENALTY=2.142251 PRIMER_INTERNAL_0_PENALTY=0.727969 PRIMER_LEFT_0_SEQUENCE=TATGAATCGCAATCAAATTTTTTC PRIMER_RIGHT_0_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_INTERNAL_0_SEQUENCE=TGACCTGATAGAAACGCCAA PRIMER_LEFT_0=30,24 PRIMER_RIGHT_0=129,18 PRIMER_INTERNAL_0=56,20 PRIMER_LEFT_0_TM=59.404 PRIMER_RIGHT_0_TM=60.142 PRIMER_INTERNAL_0_TM=59.272 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_INTERNAL_0_LIBRARY_MISHYB=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 = SEQUENCE_ID=SP01-06-with-repeat3 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MISPRIMING_LIBRARY= PRIMER_INTERNAL_MISHYB_LIBRARY=../not_for_production.lib PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=14 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_EXPLAIN=considered 136, too many Ns 36, GC content failed 1, high end compl 48, ok 51 PRIMER_RIGHT_EXPLAIN=considered 370, high end compl 191, ok 179 PRIMER_INTERNAL_EXPLAIN=considered 911, too many Ns 36, GC content failed 5, low tm 420, high tm 182, high end compl 110, ok 158 PRIMER_PAIR_EXPLAIN=considered 333, unacceptable product size 332, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=6.738411 PRIMER_LEFT_0_PENALTY=4.596160 PRIMER_RIGHT_0_PENALTY=2.142251 PRIMER_INTERNAL_0_PENALTY=0.727969 PRIMER_LEFT_0_SEQUENCE=TATGAATCGCAATCAAATTTTTTC PRIMER_RIGHT_0_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_INTERNAL_0_SEQUENCE=TGACCTGATAGAAACGCCAA PRIMER_LEFT_0=30,24 PRIMER_RIGHT_0=129,18 PRIMER_INTERNAL_0=56,20 PRIMER_LEFT_0_TM=59.404 PRIMER_RIGHT_0_TM=60.142 PRIMER_INTERNAL_0_TM=59.272 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_INTERNAL_0_LIBRARY_MISHYB=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 = SEQUENCE_ID=case_problem_test PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MISPRIMING_LIBRARY= PRIMER_INTERNAL_MISHYB_LIBRARY= SEQUENCE_TEMPLATE=atggccaaagccgcggcagtcggcatcgacctgggcaccacctactcctgcgtgggggtgttccaacacggcaaggtggagatcatcgccaacgaccagggcaaccgcaccacccccagctacgtggccttcacggacaccgagcggctcatcggggatgcggccaagaaccaggtggcgctgaacccgcagaacaccgtgtttgacgcgaagcgcctgatcggccgcaagttcggcgacccggtggtgcagtcggacatgaagcactggcctttccaggtgatcaacgacggagacaagcccaaggtgcaggtgagctacaagggggagaccaaggcattctaccccgaggagatctcgtccatggtgctgaccaagatgaaggagatcgccgaggcgtacctgggctacccggtgaccaacgcggtgatcaccgtgccggcctacttcaacgactcgcagcgccaggccaccaaggatgcgggtgtgatcgcggggctcaacgtgctgcggatcatcaacgagcccacggccgccgccatcgcctacggcctggacagaacgggcaagggggagcgcaacgtcctgatctttgacctgggcgggggcaccttcgacgtgtccatcctgacgatcgacgacggcatcttcgaggtgaaggccacggccggggacacccacctgggtggggaggactttgacaacaggctggtgaaccacttcgtggaggagttcaagagaaaacacaagaaggacatcagccagaacaagcgagccgtgaggcggctgcgcaccgcctgcgagagggccaagaggaccctgtcgtccagcacccaggccagcctggagatcgactccctgtttgagggcatcgacttctacacgtccatcaccagggcgaggttcgaggagctgtgctccgacctgttccgaagcaccctggagcccgtggagaaggctctgcgcgacgccaagctggacaaggcccagattcacgacctggtcctggtcgggggctccacccgcatccccaaggtgcagaagctgctgcaggacttcttcaacgggcgcgacctgaacaagagcatcaaccccgacgaggctgtgggctacggggcggcggtgcaggcggccatcctgatgggggacaagtccgagaacgtgcaggacctgctgctgctggacgtggctcccctgtcgctggggctggagacggccggaggcgtgatgactgccctgatcaagcgcaactccaccatccccaccaagcagacgcagatcttcaccacctactccgacaaccaacccggggtgctgatccaggtgtacgagggcgagagggccatgacgaaagacaacaatctgttggggcgcttcgagctgagcggcatccctccggccccaggcgtgccccagatcgaggtgaccttcgacatcgatgccaacggcatcctgaacgtcacggccacggacaagagcaccggcaaggccaacaagatcaccatcaccaacgacaagggccgcctgagcaaggaggagatcgagcgcatggtgcaggaggcggagaagtacaaagcggaggacgaggtgcagcgcgagagggtgtcagccaagaacgccctggagtcctacgccttcaacatgaagagcgccgtggaggatgaggggctcaagggcaagatcagcgaggccgacaagaagaaggtgctggacaagtgtcaagaggtcatctcgtggctggacgccaacaccttggccgagaaggacgagtttgagcacaagaggaaggagctggagcaggtgtgtaaccccatcatcagcggactgtaccagggtgccggtggtcccgggcctgggggcttcggggctcagggtcccaagggagggtctgggtcaggccccaccattgaggaggtagat SEQUENCE_PRIMER=acctgggcaccacctactc PRIMER_PRODUCT_SIZE_RANGE=1890-1920 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 310, high end compl 194, ok 116 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=1.103015 PRIMER_LEFT_0_PENALTY=1.022702 PRIMER_RIGHT_0_PENALTY=0.080313 PRIMER_LEFT_0_SEQUENCE=acctgggcaccacctactc PRIMER_RIGHT_0_SEQUENCE=tctacctcctcaatggtggg PRIMER_LEFT_0=28,19 PRIMER_RIGHT_0=1918,20 PRIMER_LEFT_0_TM=59.977 PRIMER_RIGHT_0_TM=59.920 PRIMER_LEFT_0_GC_PERCENT=63.158 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=5.4000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=1891 = SEQUENCE_ID=tm_out_of_range_on_input PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MAX_TM=59.97 PRIMER_OPT_TM=59.97 SEQUENCE_TEMPLATE=atggccaaagccgcggcagtcggcatcgacctgggcaccacctactcctgcgtgggggtgttccaacacggcaaggtggagatcatcgccaacgaccagggcaaccgcaccacccccagctacgtggccttcacggacaccgagcggctcatcggggatgcggccaagaaccaggtggcgctgaacccgcagaacaccgtgtttgacgcgaagcgcctgatcggccgcaagttcggcgacccggtggtgcagtcggacatgaagcactggcctttccaggtgatcaacgacggagacaagcccaaggtgcaggtgagctacaagggggagaccaaggcattctaccccgaggagatctcgtccatggtgctgaccaagatgaaggagatcgccgaggcgtacctgggctacccggtgaccaacgcggtgatcaccgtgccggcctacttcaacgactcgcagcgccaggccaccaaggatgcgggtgtgatcgcggggctcaacgtgctgcggatcatcaacgagcccacggccgccgccatcgcctacggcctggacagaacgggcaagggggagcgcaacgtcctgatctttgacctgggcgggggcaccttcgacgtgtccatcctgacgatcgacgacggcatcttcgaggtgaaggccacggccggggacacccacctgggtggggaggactttgacaacaggctggtgaaccacttcgtggaggagttcaagagaaaacacaagaaggacatcagccagaacaagcgagccgtgaggcggctgcgcaccgcctgcgagagggccaagaggaccctgtcgtccagcacccaggccagcctggagatcgactccctgtttgagggcatcgacttctacacgtccatcaccagggcgaggttcgaggagctgtgctccgacctgttccgaagcaccctggagcccgtggagaaggctctgcgcgacgccaagctggacaaggcccagattcacgacctggtcctggtcgggggctccacccgcatccccaaggtgcagaagctgctgcaggacttcttcaacgggcgcgacctgaacaagagcatcaaccccgacgaggctgtgggctacggggcggcggtgcaggcggccatcctgatgggggacaagtccgagaacgtgcaggacctgctgctgctggacgtggctcccctgtcgctggggctggagacggccggaggcgtgatgactgccctgatcaagcgcaactccaccatccccaccaagcagacgcagatcttcaccacctactccgacaaccaacccggggtgctgatccaggtgtacgagggcgagagggccatgacgaaagacaacaatctgttggggcgcttcgagctgagcggcatccctccggccccaggcgtgccccagatcgaggtgaccttcgacatcgatgccaacggcatcctgaacgtcacggccacggacaagagcaccggcaaggccaacaagatcaccatcaccaacgacaagggccgcctgagcaaggaggagatcgagcgcatggtgcaggaggcggagaagtacaaagcggaggacgaggtgcagcgcgagagggtgtcagccaagaacgccctggagtcctacgccttcaacatgaagagcgccgtggaggatgaggggctcaagggcaagatcagcgaggccgacaagaagaaggtgctggacaagtgtcaagaggtcatctcgtggctggacgccaacaccttggccgagaaggacgagtttgagcacaagaggaaggagctggagcaggtgtgtaaccccatcatcagcggactgtaccagggtgccggtggtcccgggcctgggggcttcggggctcagggtcccaagggagggtctgggtcaggccccaccattgaggaggtagat SEQUENCE_PRIMER=acctgggcaccacctactc PRIMER_PRODUCT_SIZE_RANGE=1890-1920 PRIMER_LEFT_EXPLAIN=considered 1, high tm 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 310, high tm 301, high end compl 3, ok 6 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = primer3-2.3.6/test/primer_list_tmp/0000775000077300007730000000000012231156372016247 5ustar ioanaioanaprimer3-2.3.6/test/p3_3_prime_0_output0000664000077300007730000036102512231156245016566 0ustar ioanaioanaPRIMER_MIN_THREE_PRIME_DISTANCE=0 PRIMER_NUM_RETURN=2 PRIMER_OPT_SIZE=20 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000 PRIMER_MAX_GC=80.0 PRIMER_MAX_SIZE=25 PRIMER_MAX_TM=63.0 PRIMER_MIN_SIZE=18 PRIMER_MIN_GC=20 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=6 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=6 MARKER_NAME=01.RHAP33FA1.seq SEQUENCE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN INCLUDED_REGION=20,315 TARGET=81,30,CA PRIMER_PAIR_PENALTY=7.7339 PRIMER_LEFT_PENALTY=7.538496 PRIMER_RIGHT_PENALTY=0.195358 PRIMER_LEFT_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_SEQUENCE=GGCAAAAGGACAGAGGATGA PRIMER_LEFT=20,25 PRIMER_RIGHT=177,20 PRIMER_LEFT_TM=57.462 PRIMER_RIGHT_TM=60.195 PRIMER_LEFT_GC_PERCENT=32.000 PRIMER_RIGHT_GC_PERCENT=50.000 PRIMER_LEFT_SELF_ANY=5.00 PRIMER_RIGHT_SELF_ANY=2.00 PRIMER_LEFT_SELF_END=0.00 PRIMER_RIGHT_SELF_END=1.00 PRIMER_LEFT_END_STABILITY=6.0000 PRIMER_RIGHT_END_STABILITY=6.6000 PRIMER_PAIR_MAX_COMPL_ANY=4.00 PRIMER_PAIR_MAX_COMPL_END=0.00 PRIMER_PRODUCT_SIZE=158 PRIMER_PAIR_PENALTY_1=7.8680 PRIMER_LEFT_1_PENALTY=7.538496 PRIMER_RIGHT_1_PENALTY=0.329550 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_1_SEQUENCE=ATCTTGGGCAAAAGGACAGA PRIMER_LEFT_1=20,25 PRIMER_RIGHT_1=183,20 PRIMER_LEFT_1_TM=57.462 PRIMER_RIGHT_1_TM=59.670 PRIMER_LEFT_1_GC_PERCENT=32.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.0000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_1=164 = MARKER_NAME=01.RHAP34FA1.seq SEQUENCE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT INCLUDED_REGION=19,296 TARGET=134,15,CA TARGET=213,16,AT TARGET=235,16,AT = PRIMER_NUM_RETURN=1 MARKER_NAME=03.RHAP34FA3.seq SEQUENCE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT INCLUDED_REGION=0,278 TARGET=1,28,CA = MARKER_NAME=03.RHAP34FE3.seq SEQUENCE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA INCLUDED_REGION=17,284 TARGET=27,60,AC = MARKER_NAME=04.RHAP33FE4.seq SEQUENCE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC INCLUDED_REGION=18,367 TARGET=146,46,AT TARGET=199,22,TA TARGET=286,13,GT TARGET=300,15,AG PRIMER_PAIR_PENALTY=7.3535 PRIMER_LEFT_PENALTY=4.174047 PRIMER_RIGHT_PENALTY=3.179421 PRIMER_LEFT_SEQUENCE=TGAGAAGGGAGGAGTAAGTACTGG PRIMER_RIGHT_SEQUENCE=CCTATGCCTATACACCACACACA PRIMER_LEFT=97,24 PRIMER_RIGHT=270,23 PRIMER_LEFT_TM=60.174 PRIMER_RIGHT_TM=59.821 PRIMER_LEFT_GC_PERCENT=50.000 PRIMER_RIGHT_GC_PERCENT=47.826 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=4.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=0.00 PRIMER_LEFT_END_STABILITY=7.9000 PRIMER_RIGHT_END_STABILITY=6.4000 PRIMER_PAIR_MAX_COMPL_ANY=4.00 PRIMER_PAIR_MAX_COMPL_END=1.00 PRIMER_PRODUCT_SIZE=174 = MARKER_NAME=05.RHAP33FA5.seq SEQUENCE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA INCLUDED_REGION=10,369 TARGET=30,44,GT = MARKER_NAME=05.RHAP33FE5.seq SEQUENCE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN INCLUDED_REGION=14,306 TARGET=176,34,CA PRIMER_PAIR_PENALTY=0.8585 PRIMER_LEFT_PENALTY=0.702456 PRIMER_RIGHT_PENALTY=0.156061 PRIMER_LEFT_SEQUENCE=CATGTTCGTGCAAACACTCA PRIMER_RIGHT_SEQUENCE=AGACCCTGTCTCCAAAAGCA PRIMER_LEFT=129,20 PRIMER_RIGHT=302,20 PRIMER_LEFT_TM=59.298 PRIMER_RIGHT_TM=59.844 PRIMER_LEFT_GC_PERCENT=45.000 PRIMER_RIGHT_GC_PERCENT=50.000 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=5.00 PRIMER_LEFT_SELF_END=1.00 PRIMER_RIGHT_SELF_END=0.00 PRIMER_LEFT_END_STABILITY=6.4000 PRIMER_RIGHT_END_STABILITY=8.5000 PRIMER_PAIR_MAX_COMPL_ANY=4.00 PRIMER_PAIR_MAX_COMPL_END=0.00 PRIMER_PRODUCT_SIZE=174 = PRIMER_NUM_RETURN=40 MARKER_NAME=05.RHAP34FA5.seq SEQUENCE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA INCLUDED_REGION=9,297 TARGET=125,36,AC TARGET=162,24,GA TARGET=191,16,AG TARGET=214,14,GA PRIMER_PAIR_PENALTY=9.8843 PRIMER_LEFT_PENALTY=1.973952 PRIMER_RIGHT_PENALTY=7.910390 PRIMER_LEFT_SEQUENCE=GCGGCCGACATCTACTACTA PRIMER_RIGHT_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT=103,20 PRIMER_RIGHT=268,25 PRIMER_LEFT_TM=58.026 PRIMER_RIGHT_TM=57.090 PRIMER_LEFT_GC_PERCENT=55.000 PRIMER_RIGHT_GC_PERCENT=44.000 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=3.00 PRIMER_LEFT_SELF_END=4.00 PRIMER_RIGHT_SELF_END=1.00 PRIMER_LEFT_END_STABILITY=4.7000 PRIMER_RIGHT_END_STABILITY=6.0000 PRIMER_PAIR_MAX_COMPL_ANY=4.00 PRIMER_PAIR_MAX_COMPL_END=1.00 PRIMER_PRODUCT_SIZE=166 PRIMER_PAIR_PENALTY_1=10.0123 PRIMER_LEFT_1_PENALTY=2.101866 PRIMER_RIGHT_1_PENALTY=7.910390 PRIMER_LEFT_1_SEQUENCE=GCGGCCGACATCTACTACTAC PRIMER_RIGHT_1_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_1=103,21 PRIMER_RIGHT_1=268,25 PRIMER_LEFT_1_TM=58.898 PRIMER_RIGHT_1_TM=57.090 PRIMER_LEFT_1_GC_PERCENT=57.143 PRIMER_RIGHT_1_GC_PERCENT=44.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=5.1000 PRIMER_RIGHT_1_END_STABILITY=6.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_1=166 PRIMER_PAIR_PENALTY_2=10.0792 PRIMER_LEFT_2_PENALTY=2.168831 PRIMER_RIGHT_2_PENALTY=7.910390 PRIMER_LEFT_2_SEQUENCE=GCGGCCGACATCTACTACTACT PRIMER_RIGHT_2_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_2=103,22 PRIMER_RIGHT_2=268,25 PRIMER_LEFT_2_TM=59.831 PRIMER_RIGHT_2_TM=57.090 PRIMER_LEFT_2_GC_PERCENT=54.545 PRIMER_RIGHT_2_GC_PERCENT=44.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=5.4000 PRIMER_RIGHT_2_END_STABILITY=6.0000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_2=166 PRIMER_PAIR_PENALTY_3=10.5142 PRIMER_LEFT_3_PENALTY=2.603822 PRIMER_RIGHT_3_PENALTY=7.910390 PRIMER_LEFT_3_SEQUENCE=CGCGGCCGACATCTACTACT PRIMER_RIGHT_3_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_3=102,20 PRIMER_RIGHT_3=268,25 PRIMER_LEFT_3_TM=62.604 PRIMER_RIGHT_3_TM=57.090 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=44.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=5.4000 PRIMER_RIGHT_3_END_STABILITY=6.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_3=167 PRIMER_PAIR_PENALTY_4=10.6566 PRIMER_LEFT_4_PENALTY=2.746227 PRIMER_RIGHT_4_PENALTY=7.910390 PRIMER_LEFT_4_SEQUENCE=CGCGGCCGACATCTACTAC PRIMER_RIGHT_4_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_4=102,19 PRIMER_RIGHT_4=268,25 PRIMER_LEFT_4_TM=61.746 PRIMER_RIGHT_4_TM=57.090 PRIMER_LEFT_4_GC_PERCENT=63.158 PRIMER_RIGHT_4_GC_PERCENT=44.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=5.1000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_4=167 PRIMER_PAIR_PENALTY_5=10.8467 PRIMER_LEFT_5_PENALTY=2.936346 PRIMER_RIGHT_5_PENALTY=7.910390 PRIMER_LEFT_5_SEQUENCE=CGCGGCCGACATCTACTA PRIMER_RIGHT_5_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_5=102,18 PRIMER_RIGHT_5=268,25 PRIMER_LEFT_5_TM=60.936 PRIMER_RIGHT_5_TM=57.090 PRIMER_LEFT_5_GC_PERCENT=61.111 PRIMER_RIGHT_5_GC_PERCENT=44.000 PRIMER_LEFT_5_SELF_ANY=6.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_END_STABILITY=4.7000 PRIMER_RIGHT_5_END_STABILITY=6.0000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_5=167 PRIMER_PAIR_PENALTY_6=10.9749 PRIMER_LEFT_6_PENALTY=3.064519 PRIMER_RIGHT_6_PENALTY=7.910390 PRIMER_LEFT_6_SEQUENCE=GCGGCCGACATCTACTACT PRIMER_RIGHT_6_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_6=103,19 PRIMER_RIGHT_6=268,25 PRIMER_LEFT_6_TM=57.935 PRIMER_RIGHT_6_TM=57.090 PRIMER_LEFT_6_GC_PERCENT=57.895 PRIMER_RIGHT_6_GC_PERCENT=44.000 PRIMER_LEFT_6_SELF_ANY=6.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_END_STABILITY=5.4000 PRIMER_RIGHT_6_END_STABILITY=6.0000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_6=166 PRIMER_PAIR_PENALTY_7=11.4300 PRIMER_LEFT_7_PENALTY=3.519592 PRIMER_RIGHT_7_PENALTY=7.910390 PRIMER_LEFT_7_SEQUENCE=CGCGGCCGACATCTACTACTA PRIMER_RIGHT_7_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_7=102,21 PRIMER_RIGHT_7=268,25 PRIMER_LEFT_7_TM=62.520 PRIMER_RIGHT_7_TM=57.090 PRIMER_LEFT_7_GC_PERCENT=57.143 PRIMER_RIGHT_7_GC_PERCENT=44.000 PRIMER_LEFT_7_SELF_ANY=6.00 PRIMER_RIGHT_7_SELF_ANY=3.00 PRIMER_LEFT_7_SELF_END=4.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_END_STABILITY=4.7000 PRIMER_RIGHT_7_END_STABILITY=6.0000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_7=167 PRIMER_PAIR_PENALTY_8=0.3479 PRIMER_LEFT_8_PENALTY=0.003322 PRIMER_RIGHT_8_PENALTY=0.344549 PRIMER_LEFT_8_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_8_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_8=54,20 PRIMER_RIGHT_8=294,20 PRIMER_LEFT_8_TM=59.997 PRIMER_RIGHT_8_TM=60.345 PRIMER_LEFT_8_GC_PERCENT=55.000 PRIMER_RIGHT_8_GC_PERCENT=50.000 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=7.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_LEFT_8_END_STABILITY=9.4000 PRIMER_RIGHT_8_END_STABILITY=8.2000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_8=241 PRIMER_PAIR_PENALTY_9=0.3873 PRIMER_LEFT_9_PENALTY=0.042732 PRIMER_RIGHT_9_PENALTY=0.344549 PRIMER_LEFT_9_SEQUENCE=GAAGGCCACTCTCATTCAGC PRIMER_RIGHT_9_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_9=65,20 PRIMER_RIGHT_9=294,20 PRIMER_LEFT_9_TM=59.957 PRIMER_RIGHT_9_TM=60.345 PRIMER_LEFT_9_GC_PERCENT=55.000 PRIMER_RIGHT_9_GC_PERCENT=50.000 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=7.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=0.00 PRIMER_LEFT_9_END_STABILITY=8.2000 PRIMER_RIGHT_9_END_STABILITY=8.2000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_9=230 PRIMER_PAIR_PENALTY_10=0.4783 PRIMER_LEFT_10_PENALTY=0.133741 PRIMER_RIGHT_10_PENALTY=0.344549 PRIMER_LEFT_10_SEQUENCE=AGCTGAGACTTTGAAGGCCA PRIMER_RIGHT_10_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_10=53,20 PRIMER_RIGHT_10=294,20 PRIMER_LEFT_10_TM=60.134 PRIMER_RIGHT_10_TM=60.345 PRIMER_LEFT_10_GC_PERCENT=50.000 PRIMER_RIGHT_10_GC_PERCENT=50.000 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=7.00 PRIMER_LEFT_10_SELF_END=4.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_END_STABILITY=11.2000 PRIMER_RIGHT_10_END_STABILITY=8.2000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_10=242 PRIMER_PAIR_PENALTY_11=0.4783 PRIMER_LEFT_11_PENALTY=0.133741 PRIMER_RIGHT_11_PENALTY=0.344549 PRIMER_LEFT_11_SEQUENCE=CAGCTGAGACTTTGAAGGCC PRIMER_RIGHT_11_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_11=52,20 PRIMER_RIGHT_11=294,20 PRIMER_LEFT_11_TM=60.134 PRIMER_RIGHT_11_TM=60.345 PRIMER_LEFT_11_GC_PERCENT=55.000 PRIMER_RIGHT_11_GC_PERCENT=50.000 PRIMER_LEFT_11_SELF_ANY=6.00 PRIMER_RIGHT_11_SELF_ANY=7.00 PRIMER_LEFT_11_SELF_END=4.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_END_STABILITY=10.9000 PRIMER_RIGHT_11_END_STABILITY=8.2000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_11=243 PRIMER_PAIR_PENALTY_12=0.6053 PRIMER_LEFT_12_PENALTY=0.260757 PRIMER_RIGHT_12_PENALTY=0.344549 PRIMER_LEFT_12_SEQUENCE=CCACTCTCATTCAGCCAGGT PRIMER_RIGHT_12_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_12=70,20 PRIMER_RIGHT_12=294,20 PRIMER_LEFT_12_TM=60.261 PRIMER_RIGHT_12_TM=60.345 PRIMER_LEFT_12_GC_PERCENT=55.000 PRIMER_RIGHT_12_GC_PERCENT=50.000 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=7.00 PRIMER_LEFT_12_SELF_END=1.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_END_STABILITY=7.9000 PRIMER_RIGHT_12_END_STABILITY=8.2000 PRIMER_PAIR_12_COMPL_ANY=3.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_12=225 PRIMER_PAIR_PENALTY_13=0.6297 PRIMER_LEFT_13_PENALTY=0.285121 PRIMER_RIGHT_13_PENALTY=0.344549 PRIMER_LEFT_13_SEQUENCE=GCAGCTGAGACTTTGAAGGC PRIMER_RIGHT_13_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_13=51,20 PRIMER_RIGHT_13=294,20 PRIMER_LEFT_13_TM=60.285 PRIMER_RIGHT_13_TM=60.345 PRIMER_LEFT_13_GC_PERCENT=55.000 PRIMER_RIGHT_13_GC_PERCENT=50.000 PRIMER_LEFT_13_SELF_ANY=6.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_LEFT_13_SELF_END=4.00 PRIMER_RIGHT_13_SELF_END=0.00 PRIMER_LEFT_13_END_STABILITY=9.7000 PRIMER_RIGHT_13_END_STABILITY=8.2000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_13=244 PRIMER_PAIR_PENALTY_14=0.6540 PRIMER_LEFT_14_PENALTY=0.309402 PRIMER_RIGHT_14_PENALTY=0.344549 PRIMER_LEFT_14_SEQUENCE=CATTCAGCCAGGTGTGTGAG PRIMER_RIGHT_14_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_14=77,20 PRIMER_RIGHT_14=294,20 PRIMER_LEFT_14_TM=60.309 PRIMER_RIGHT_14_TM=60.345 PRIMER_LEFT_14_GC_PERCENT=55.000 PRIMER_RIGHT_14_GC_PERCENT=50.000 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=7.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_LEFT_14_END_STABILITY=6.4000 PRIMER_RIGHT_14_END_STABILITY=8.2000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_14=218 PRIMER_PAIR_PENALTY_15=0.6540 PRIMER_LEFT_15_PENALTY=0.309402 PRIMER_RIGHT_15_PENALTY=0.344549 PRIMER_LEFT_15_SEQUENCE=CTCATTCAGCCAGGTGTGTG PRIMER_RIGHT_15_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_15=75,20 PRIMER_RIGHT_15=294,20 PRIMER_LEFT_15_TM=60.309 PRIMER_RIGHT_15_TM=60.345 PRIMER_LEFT_15_GC_PERCENT=55.000 PRIMER_RIGHT_15_GC_PERCENT=50.000 PRIMER_LEFT_15_SELF_ANY=3.00 PRIMER_RIGHT_15_SELF_ANY=7.00 PRIMER_LEFT_15_SELF_END=3.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_END_STABILITY=6.4000 PRIMER_RIGHT_15_END_STABILITY=8.2000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_15=220 PRIMER_PAIR_PENALTY_16=0.9299 PRIMER_LEFT_16_PENALTY=0.585389 PRIMER_RIGHT_16_PENALTY=0.344549 PRIMER_LEFT_16_SEQUENCE=CTCTCATTCAGCCAGGTGTG PRIMER_RIGHT_16_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_16=73,20 PRIMER_RIGHT_16=294,20 PRIMER_LEFT_16_TM=59.415 PRIMER_RIGHT_16_TM=60.345 PRIMER_LEFT_16_GC_PERCENT=55.000 PRIMER_RIGHT_16_GC_PERCENT=50.000 PRIMER_LEFT_16_SELF_ANY=3.00 PRIMER_RIGHT_16_SELF_ANY=7.00 PRIMER_LEFT_16_SELF_END=1.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_END_STABILITY=6.4000 PRIMER_RIGHT_16_END_STABILITY=8.2000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_16=222 PRIMER_PAIR_PENALTY_17=0.9299 PRIMER_LEFT_17_PENALTY=0.585389 PRIMER_RIGHT_17_PENALTY=0.344549 PRIMER_LEFT_17_SEQUENCE=CACTCTCATTCAGCCAGGTG PRIMER_RIGHT_17_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_17=71,20 PRIMER_RIGHT_17=294,20 PRIMER_LEFT_17_TM=59.415 PRIMER_RIGHT_17_TM=60.345 PRIMER_LEFT_17_GC_PERCENT=55.000 PRIMER_RIGHT_17_GC_PERCENT=50.000 PRIMER_LEFT_17_SELF_ANY=3.00 PRIMER_RIGHT_17_SELF_ANY=7.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_END_STABILITY=7.9000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_17=224 PRIMER_PAIR_PENALTY_18=0.9758 PRIMER_LEFT_18_PENALTY=0.631211 PRIMER_RIGHT_18_PENALTY=0.344549 PRIMER_LEFT_18_SEQUENCE=TTGAAGGCCACTCTCATTCA PRIMER_RIGHT_18_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_18=63,20 PRIMER_RIGHT_18=294,20 PRIMER_LEFT_18_TM=59.369 PRIMER_RIGHT_18_TM=60.345 PRIMER_LEFT_18_GC_PERCENT=45.000 PRIMER_RIGHT_18_GC_PERCENT=50.000 PRIMER_LEFT_18_SELF_ANY=5.00 PRIMER_RIGHT_18_SELF_ANY=7.00 PRIMER_LEFT_18_SELF_END=5.00 PRIMER_RIGHT_18_SELF_END=0.00 PRIMER_LEFT_18_END_STABILITY=6.9000 PRIMER_RIGHT_18_END_STABILITY=8.2000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_18=232 PRIMER_PAIR_PENALTY_19=1.0308 PRIMER_LEFT_19_PENALTY=0.686256 PRIMER_RIGHT_19_PENALTY=0.344549 PRIMER_LEFT_19_SEQUENCE=TGCAGCTGAGACTTTGAAGG PRIMER_RIGHT_19_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_19=50,20 PRIMER_RIGHT_19=294,20 PRIMER_LEFT_19_TM=59.314 PRIMER_RIGHT_19_TM=60.345 PRIMER_LEFT_19_GC_PERCENT=50.000 PRIMER_RIGHT_19_GC_PERCENT=50.000 PRIMER_LEFT_19_SELF_ANY=6.00 PRIMER_RIGHT_19_SELF_ANY=7.00 PRIMER_LEFT_19_SELF_END=3.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_END_STABILITY=8.2000 PRIMER_RIGHT_19_END_STABILITY=8.2000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_19=245 PRIMER_PAIR_PENALTY_20=1.0833 PRIMER_LEFT_20_PENALTY=0.738752 PRIMER_RIGHT_20_PENALTY=0.344549 PRIMER_LEFT_20_SEQUENCE=TCTCATTCAGCCAGGTGTGT PRIMER_RIGHT_20_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_20=74,20 PRIMER_RIGHT_20=294,20 PRIMER_LEFT_20_TM=59.261 PRIMER_RIGHT_20_TM=60.345 PRIMER_LEFT_20_GC_PERCENT=50.000 PRIMER_RIGHT_20_GC_PERCENT=50.000 PRIMER_LEFT_20_SELF_ANY=3.00 PRIMER_RIGHT_20_SELF_ANY=7.00 PRIMER_LEFT_20_SELF_END=1.00 PRIMER_RIGHT_20_SELF_END=0.00 PRIMER_LEFT_20_END_STABILITY=6.4000 PRIMER_RIGHT_20_END_STABILITY=8.2000 PRIMER_PAIR_20_COMPL_ANY=4.00 PRIMER_PAIR_20_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_20=221 PRIMER_PAIR_PENALTY_21=1.1833 PRIMER_LEFT_21_PENALTY=0.003322 PRIMER_RIGHT_21_PENALTY=1.180017 PRIMER_LEFT_21_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_21_SEQUENCE=GTCCTTCCCACAACCAACCT PRIMER_LEFT_21=54,20 PRIMER_RIGHT_21=290,20 PRIMER_LEFT_21_TM=59.997 PRIMER_RIGHT_21_TM=61.180 PRIMER_LEFT_21_GC_PERCENT=55.000 PRIMER_RIGHT_21_GC_PERCENT=55.000 PRIMER_LEFT_21_SELF_ANY=4.00 PRIMER_RIGHT_21_SELF_ANY=2.00 PRIMER_LEFT_21_SELF_END=3.00 PRIMER_RIGHT_21_SELF_END=0.00 PRIMER_LEFT_21_END_STABILITY=9.4000 PRIMER_RIGHT_21_END_STABILITY=7.9000 PRIMER_PAIR_21_COMPL_ANY=5.00 PRIMER_PAIR_21_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_21=237 PRIMER_PAIR_PENALTY_22=1.1833 PRIMER_LEFT_22_PENALTY=0.003322 PRIMER_RIGHT_22_PENALTY=1.180017 PRIMER_LEFT_22_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_22_SEQUENCE=AGGTCCTTCCCACAACCAAC PRIMER_LEFT_22=54,20 PRIMER_RIGHT_22=292,20 PRIMER_LEFT_22_TM=59.997 PRIMER_RIGHT_22_TM=61.180 PRIMER_LEFT_22_GC_PERCENT=55.000 PRIMER_RIGHT_22_GC_PERCENT=55.000 PRIMER_LEFT_22_SELF_ANY=4.00 PRIMER_RIGHT_22_SELF_ANY=5.00 PRIMER_LEFT_22_SELF_END=3.00 PRIMER_RIGHT_22_SELF_END=0.00 PRIMER_LEFT_22_END_STABILITY=9.4000 PRIMER_RIGHT_22_END_STABILITY=8.2000 PRIMER_PAIR_22_COMPL_ANY=5.00 PRIMER_PAIR_22_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_22=239 PRIMER_PAIR_PENALTY_23=1.2102 PRIMER_LEFT_23_PENALTY=0.003322 PRIMER_RIGHT_23_PENALTY=1.206859 PRIMER_LEFT_23_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_23_SEQUENCE=GTCCTTCCCACAACCAACC PRIMER_LEFT_23=54,20 PRIMER_RIGHT_23=290,19 PRIMER_LEFT_23_TM=59.997 PRIMER_RIGHT_23_TM=60.207 PRIMER_LEFT_23_GC_PERCENT=55.000 PRIMER_RIGHT_23_GC_PERCENT=57.895 PRIMER_LEFT_23_SELF_ANY=4.00 PRIMER_RIGHT_23_SELF_ANY=2.00 PRIMER_LEFT_23_SELF_END=3.00 PRIMER_RIGHT_23_SELF_END=0.00 PRIMER_LEFT_23_END_STABILITY=9.4000 PRIMER_RIGHT_23_END_STABILITY=8.2000 PRIMER_PAIR_23_COMPL_ANY=5.00 PRIMER_PAIR_23_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_23=237 PRIMER_PAIR_PENALTY_24=1.2102 PRIMER_LEFT_24_PENALTY=0.003322 PRIMER_RIGHT_24_PENALTY=1.206859 PRIMER_LEFT_24_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_24_SEQUENCE=GGTCCTTCCCACAACCAAC PRIMER_LEFT_24=54,20 PRIMER_RIGHT_24=291,19 PRIMER_LEFT_24_TM=59.997 PRIMER_RIGHT_24_TM=60.207 PRIMER_LEFT_24_GC_PERCENT=55.000 PRIMER_RIGHT_24_GC_PERCENT=57.895 PRIMER_LEFT_24_SELF_ANY=4.00 PRIMER_RIGHT_24_SELF_ANY=3.00 PRIMER_LEFT_24_SELF_END=3.00 PRIMER_RIGHT_24_SELF_END=0.00 PRIMER_LEFT_24_END_STABILITY=9.4000 PRIMER_RIGHT_24_END_STABILITY=8.2000 PRIMER_PAIR_24_COMPL_ANY=5.00 PRIMER_PAIR_24_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_24=238 PRIMER_PAIR_PENALTY_25=1.2227 PRIMER_LEFT_25_PENALTY=0.042732 PRIMER_RIGHT_25_PENALTY=1.180017 PRIMER_LEFT_25_SEQUENCE=GAAGGCCACTCTCATTCAGC PRIMER_RIGHT_25_SEQUENCE=GTCCTTCCCACAACCAACCT PRIMER_LEFT_25=65,20 PRIMER_RIGHT_25=290,20 PRIMER_LEFT_25_TM=59.957 PRIMER_RIGHT_25_TM=61.180 PRIMER_LEFT_25_GC_PERCENT=55.000 PRIMER_RIGHT_25_GC_PERCENT=55.000 PRIMER_LEFT_25_SELF_ANY=4.00 PRIMER_RIGHT_25_SELF_ANY=2.00 PRIMER_LEFT_25_SELF_END=2.00 PRIMER_RIGHT_25_SELF_END=0.00 PRIMER_LEFT_25_END_STABILITY=8.2000 PRIMER_RIGHT_25_END_STABILITY=7.9000 PRIMER_PAIR_25_COMPL_ANY=5.00 PRIMER_PAIR_25_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_25=226 PRIMER_PAIR_PENALTY_26=1.2227 PRIMER_LEFT_26_PENALTY=0.042732 PRIMER_RIGHT_26_PENALTY=1.180017 PRIMER_LEFT_26_SEQUENCE=GAAGGCCACTCTCATTCAGC PRIMER_RIGHT_26_SEQUENCE=AGGTCCTTCCCACAACCAAC PRIMER_LEFT_26=65,20 PRIMER_RIGHT_26=292,20 PRIMER_LEFT_26_TM=59.957 PRIMER_RIGHT_26_TM=61.180 PRIMER_LEFT_26_GC_PERCENT=55.000 PRIMER_RIGHT_26_GC_PERCENT=55.000 PRIMER_LEFT_26_SELF_ANY=4.00 PRIMER_RIGHT_26_SELF_ANY=5.00 PRIMER_LEFT_26_SELF_END=2.00 PRIMER_RIGHT_26_SELF_END=0.00 PRIMER_LEFT_26_END_STABILITY=8.2000 PRIMER_RIGHT_26_END_STABILITY=8.2000 PRIMER_PAIR_26_COMPL_ANY=5.00 PRIMER_PAIR_26_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_26=228 PRIMER_PAIR_PENALTY_27=1.2496 PRIMER_LEFT_27_PENALTY=0.042732 PRIMER_RIGHT_27_PENALTY=1.206859 PRIMER_LEFT_27_SEQUENCE=GAAGGCCACTCTCATTCAGC PRIMER_RIGHT_27_SEQUENCE=GTCCTTCCCACAACCAACC PRIMER_LEFT_27=65,20 PRIMER_RIGHT_27=290,19 PRIMER_LEFT_27_TM=59.957 PRIMER_RIGHT_27_TM=60.207 PRIMER_LEFT_27_GC_PERCENT=55.000 PRIMER_RIGHT_27_GC_PERCENT=57.895 PRIMER_LEFT_27_SELF_ANY=4.00 PRIMER_RIGHT_27_SELF_ANY=2.00 PRIMER_LEFT_27_SELF_END=2.00 PRIMER_RIGHT_27_SELF_END=0.00 PRIMER_LEFT_27_END_STABILITY=8.2000 PRIMER_RIGHT_27_END_STABILITY=8.2000 PRIMER_PAIR_27_COMPL_ANY=5.00 PRIMER_PAIR_27_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_27=226 PRIMER_PAIR_PENALTY_28=1.2496 PRIMER_LEFT_28_PENALTY=0.042732 PRIMER_RIGHT_28_PENALTY=1.206859 PRIMER_LEFT_28_SEQUENCE=GAAGGCCACTCTCATTCAGC PRIMER_RIGHT_28_SEQUENCE=GGTCCTTCCCACAACCAAC PRIMER_LEFT_28=65,20 PRIMER_RIGHT_28=291,19 PRIMER_LEFT_28_TM=59.957 PRIMER_RIGHT_28_TM=60.207 PRIMER_LEFT_28_GC_PERCENT=55.000 PRIMER_RIGHT_28_GC_PERCENT=57.895 PRIMER_LEFT_28_SELF_ANY=4.00 PRIMER_RIGHT_28_SELF_ANY=3.00 PRIMER_LEFT_28_SELF_END=2.00 PRIMER_RIGHT_28_SELF_END=0.00 PRIMER_LEFT_28_END_STABILITY=8.2000 PRIMER_RIGHT_28_END_STABILITY=8.2000 PRIMER_PAIR_28_COMPL_ANY=5.00 PRIMER_PAIR_28_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_28=227 PRIMER_PAIR_PENALTY_29=1.3138 PRIMER_LEFT_29_PENALTY=0.133741 PRIMER_RIGHT_29_PENALTY=1.180017 PRIMER_LEFT_29_SEQUENCE=AGCTGAGACTTTGAAGGCCA PRIMER_RIGHT_29_SEQUENCE=GTCCTTCCCACAACCAACCT PRIMER_LEFT_29=53,20 PRIMER_RIGHT_29=290,20 PRIMER_LEFT_29_TM=60.134 PRIMER_RIGHT_29_TM=61.180 PRIMER_LEFT_29_GC_PERCENT=50.000 PRIMER_RIGHT_29_GC_PERCENT=55.000 PRIMER_LEFT_29_SELF_ANY=4.00 PRIMER_RIGHT_29_SELF_ANY=2.00 PRIMER_LEFT_29_SELF_END=4.00 PRIMER_RIGHT_29_SELF_END=0.00 PRIMER_LEFT_29_END_STABILITY=11.2000 PRIMER_RIGHT_29_END_STABILITY=7.9000 PRIMER_PAIR_29_COMPL_ANY=5.00 PRIMER_PAIR_29_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_29=238 PRIMER_PAIR_PENALTY_30=1.3138 PRIMER_LEFT_30_PENALTY=0.133741 PRIMER_RIGHT_30_PENALTY=1.180017 PRIMER_LEFT_30_SEQUENCE=CAGCTGAGACTTTGAAGGCC PRIMER_RIGHT_30_SEQUENCE=GTCCTTCCCACAACCAACCT PRIMER_LEFT_30=52,20 PRIMER_RIGHT_30=290,20 PRIMER_LEFT_30_TM=60.134 PRIMER_RIGHT_30_TM=61.180 PRIMER_LEFT_30_GC_PERCENT=55.000 PRIMER_RIGHT_30_GC_PERCENT=55.000 PRIMER_LEFT_30_SELF_ANY=6.00 PRIMER_RIGHT_30_SELF_ANY=2.00 PRIMER_LEFT_30_SELF_END=4.00 PRIMER_RIGHT_30_SELF_END=0.00 PRIMER_LEFT_30_END_STABILITY=10.9000 PRIMER_RIGHT_30_END_STABILITY=7.9000 PRIMER_PAIR_30_COMPL_ANY=5.00 PRIMER_PAIR_30_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_30=239 PRIMER_PAIR_PENALTY_31=1.3138 PRIMER_LEFT_31_PENALTY=0.133741 PRIMER_RIGHT_31_PENALTY=1.180017 PRIMER_LEFT_31_SEQUENCE=AGCTGAGACTTTGAAGGCCA PRIMER_RIGHT_31_SEQUENCE=AGGTCCTTCCCACAACCAAC PRIMER_LEFT_31=53,20 PRIMER_RIGHT_31=292,20 PRIMER_LEFT_31_TM=60.134 PRIMER_RIGHT_31_TM=61.180 PRIMER_LEFT_31_GC_PERCENT=50.000 PRIMER_RIGHT_31_GC_PERCENT=55.000 PRIMER_LEFT_31_SELF_ANY=4.00 PRIMER_RIGHT_31_SELF_ANY=5.00 PRIMER_LEFT_31_SELF_END=4.00 PRIMER_RIGHT_31_SELF_END=0.00 PRIMER_LEFT_31_END_STABILITY=11.2000 PRIMER_RIGHT_31_END_STABILITY=8.2000 PRIMER_PAIR_31_COMPL_ANY=5.00 PRIMER_PAIR_31_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_31=240 PRIMER_PAIR_PENALTY_32=1.3138 PRIMER_LEFT_32_PENALTY=0.133741 PRIMER_RIGHT_32_PENALTY=1.180017 PRIMER_LEFT_32_SEQUENCE=CAGCTGAGACTTTGAAGGCC PRIMER_RIGHT_32_SEQUENCE=AGGTCCTTCCCACAACCAAC PRIMER_LEFT_32=52,20 PRIMER_RIGHT_32=292,20 PRIMER_LEFT_32_TM=60.134 PRIMER_RIGHT_32_TM=61.180 PRIMER_LEFT_32_GC_PERCENT=55.000 PRIMER_RIGHT_32_GC_PERCENT=55.000 PRIMER_LEFT_32_SELF_ANY=6.00 PRIMER_RIGHT_32_SELF_ANY=5.00 PRIMER_LEFT_32_SELF_END=4.00 PRIMER_RIGHT_32_SELF_END=0.00 PRIMER_LEFT_32_END_STABILITY=10.9000 PRIMER_RIGHT_32_END_STABILITY=8.2000 PRIMER_PAIR_32_COMPL_ANY=5.00 PRIMER_PAIR_32_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_32=241 PRIMER_PAIR_PENALTY_33=1.3406 PRIMER_LEFT_33_PENALTY=0.133741 PRIMER_RIGHT_33_PENALTY=1.206859 PRIMER_LEFT_33_SEQUENCE=AGCTGAGACTTTGAAGGCCA PRIMER_RIGHT_33_SEQUENCE=GTCCTTCCCACAACCAACC PRIMER_LEFT_33=53,20 PRIMER_RIGHT_33=290,19 PRIMER_LEFT_33_TM=60.134 PRIMER_RIGHT_33_TM=60.207 PRIMER_LEFT_33_GC_PERCENT=50.000 PRIMER_RIGHT_33_GC_PERCENT=57.895 PRIMER_LEFT_33_SELF_ANY=4.00 PRIMER_RIGHT_33_SELF_ANY=2.00 PRIMER_LEFT_33_SELF_END=4.00 PRIMER_RIGHT_33_SELF_END=0.00 PRIMER_LEFT_33_END_STABILITY=11.2000 PRIMER_RIGHT_33_END_STABILITY=8.2000 PRIMER_PAIR_33_COMPL_ANY=5.00 PRIMER_PAIR_33_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_33=238 PRIMER_PAIR_PENALTY_34=1.3406 PRIMER_LEFT_34_PENALTY=0.133741 PRIMER_RIGHT_34_PENALTY=1.206859 PRIMER_LEFT_34_SEQUENCE=CAGCTGAGACTTTGAAGGCC PRIMER_RIGHT_34_SEQUENCE=GTCCTTCCCACAACCAACC PRIMER_LEFT_34=52,20 PRIMER_RIGHT_34=290,19 PRIMER_LEFT_34_TM=60.134 PRIMER_RIGHT_34_TM=60.207 PRIMER_LEFT_34_GC_PERCENT=55.000 PRIMER_RIGHT_34_GC_PERCENT=57.895 PRIMER_LEFT_34_SELF_ANY=6.00 PRIMER_RIGHT_34_SELF_ANY=2.00 PRIMER_LEFT_34_SELF_END=4.00 PRIMER_RIGHT_34_SELF_END=0.00 PRIMER_LEFT_34_END_STABILITY=10.9000 PRIMER_RIGHT_34_END_STABILITY=8.2000 PRIMER_PAIR_34_COMPL_ANY=5.00 PRIMER_PAIR_34_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_34=239 PRIMER_PAIR_PENALTY_35=1.3406 PRIMER_LEFT_35_PENALTY=0.133741 PRIMER_RIGHT_35_PENALTY=1.206859 PRIMER_LEFT_35_SEQUENCE=AGCTGAGACTTTGAAGGCCA PRIMER_RIGHT_35_SEQUENCE=GGTCCTTCCCACAACCAAC PRIMER_LEFT_35=53,20 PRIMER_RIGHT_35=291,19 PRIMER_LEFT_35_TM=60.134 PRIMER_RIGHT_35_TM=60.207 PRIMER_LEFT_35_GC_PERCENT=50.000 PRIMER_RIGHT_35_GC_PERCENT=57.895 PRIMER_LEFT_35_SELF_ANY=4.00 PRIMER_RIGHT_35_SELF_ANY=3.00 PRIMER_LEFT_35_SELF_END=4.00 PRIMER_RIGHT_35_SELF_END=0.00 PRIMER_LEFT_35_END_STABILITY=11.2000 PRIMER_RIGHT_35_END_STABILITY=8.2000 PRIMER_PAIR_35_COMPL_ANY=5.00 PRIMER_PAIR_35_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_35=239 PRIMER_PAIR_PENALTY_36=1.3406 PRIMER_LEFT_36_PENALTY=0.133741 PRIMER_RIGHT_36_PENALTY=1.206859 PRIMER_LEFT_36_SEQUENCE=CAGCTGAGACTTTGAAGGCC PRIMER_RIGHT_36_SEQUENCE=GGTCCTTCCCACAACCAAC PRIMER_LEFT_36=52,20 PRIMER_RIGHT_36=291,19 PRIMER_LEFT_36_TM=60.134 PRIMER_RIGHT_36_TM=60.207 PRIMER_LEFT_36_GC_PERCENT=55.000 PRIMER_RIGHT_36_GC_PERCENT=57.895 PRIMER_LEFT_36_SELF_ANY=6.00 PRIMER_RIGHT_36_SELF_ANY=3.00 PRIMER_LEFT_36_SELF_END=4.00 PRIMER_RIGHT_36_SELF_END=0.00 PRIMER_LEFT_36_END_STABILITY=10.9000 PRIMER_RIGHT_36_END_STABILITY=8.2000 PRIMER_PAIR_36_COMPL_ANY=5.00 PRIMER_PAIR_36_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_36=240 PRIMER_PAIR_PENALTY_37=1.3504 PRIMER_LEFT_37_PENALTY=0.003322 PRIMER_RIGHT_37_PENALTY=1.347100 PRIMER_LEFT_37_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_37_SEQUENCE=TCCTTCCCACAACCAACCT PRIMER_LEFT_37=54,20 PRIMER_RIGHT_37=289,19 PRIMER_LEFT_37_TM=59.997 PRIMER_RIGHT_37_TM=60.347 PRIMER_LEFT_37_GC_PERCENT=55.000 PRIMER_RIGHT_37_GC_PERCENT=52.632 PRIMER_LEFT_37_SELF_ANY=4.00 PRIMER_RIGHT_37_SELF_ANY=2.00 PRIMER_LEFT_37_SELF_END=3.00 PRIMER_RIGHT_37_SELF_END=0.00 PRIMER_LEFT_37_END_STABILITY=9.4000 PRIMER_RIGHT_37_END_STABILITY=7.9000 PRIMER_PAIR_37_COMPL_ANY=5.00 PRIMER_PAIR_37_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_37=236 PRIMER_PAIR_PENALTY_38=1.3504 PRIMER_LEFT_38_PENALTY=0.003322 PRIMER_RIGHT_38_PENALTY=1.347100 PRIMER_LEFT_38_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_38_SEQUENCE=AGGTCCTTCCCACAACCAA PRIMER_LEFT_38=54,20 PRIMER_RIGHT_38=292,19 PRIMER_LEFT_38_TM=59.997 PRIMER_RIGHT_38_TM=60.347 PRIMER_LEFT_38_GC_PERCENT=55.000 PRIMER_RIGHT_38_GC_PERCENT=52.632 PRIMER_LEFT_38_SELF_ANY=4.00 PRIMER_RIGHT_38_SELF_ANY=5.00 PRIMER_LEFT_38_SELF_END=3.00 PRIMER_RIGHT_38_SELF_END=0.00 PRIMER_LEFT_38_END_STABILITY=9.4000 PRIMER_RIGHT_38_END_STABILITY=8.2000 PRIMER_PAIR_38_COMPL_ANY=5.00 PRIMER_PAIR_38_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_38=239 PRIMER_PAIR_PENALTY_39=1.3504 PRIMER_LEFT_39_PENALTY=0.003322 PRIMER_RIGHT_39_PENALTY=1.347100 PRIMER_LEFT_39_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_39_SEQUENCE=AAGGTCCTTCCCACAACCA PRIMER_LEFT_39=54,20 PRIMER_RIGHT_39=293,19 PRIMER_LEFT_39_TM=59.997 PRIMER_RIGHT_39_TM=60.347 PRIMER_LEFT_39_GC_PERCENT=55.000 PRIMER_RIGHT_39_GC_PERCENT=52.632 PRIMER_LEFT_39_SELF_ANY=4.00 PRIMER_RIGHT_39_SELF_ANY=7.00 PRIMER_LEFT_39_SELF_END=3.00 PRIMER_RIGHT_39_SELF_END=0.00 PRIMER_LEFT_39_END_STABILITY=9.4000 PRIMER_RIGHT_39_END_STABILITY=8.2000 PRIMER_PAIR_39_COMPL_ANY=5.00 PRIMER_PAIR_39_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_39=240 = MARKER_NAME=06.RHAP34FE6.seq SEQUENCE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA INCLUDED_REGION=8,287 TARGET=61,68,CA PRIMER_PAIR_PENALTY=2.0965 PRIMER_LEFT_PENALTY=1.153194 PRIMER_RIGHT_PENALTY=0.943292 PRIMER_LEFT_SEQUENCE=CAACCCAAAAATACAGACGGA PRIMER_RIGHT_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT=9,21 PRIMER_RIGHT=148,20 PRIMER_LEFT_TM=59.847 PRIMER_RIGHT_TM=60.943 PRIMER_LEFT_GC_PERCENT=42.857 PRIMER_RIGHT_GC_PERCENT=55.000 PRIMER_LEFT_SELF_ANY=2.00 PRIMER_RIGHT_SELF_ANY=3.00 PRIMER_LEFT_SELF_END=0.00 PRIMER_RIGHT_SELF_END=0.00 PRIMER_LEFT_END_STABILITY=9.6000 PRIMER_RIGHT_END_STABILITY=7.0000 PRIMER_PAIR_MAX_COMPL_ANY=6.00 PRIMER_PAIR_MAX_COMPL_END=4.00 PRIMER_PRODUCT_SIZE=140 PRIMER_PAIR_PENALTY_1=2.6236 PRIMER_LEFT_1_PENALTY=1.153194 PRIMER_RIGHT_1_PENALTY=1.470379 PRIMER_LEFT_1_SEQUENCE=CAACCCAAAAATACAGACGGA PRIMER_RIGHT_1_SEQUENCE=CCCTGTGTTACCCGTCTCA PRIMER_LEFT_1=9,21 PRIMER_RIGHT_1=148,19 PRIMER_LEFT_1_TM=59.847 PRIMER_RIGHT_1_TM=59.530 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_1=140 PRIMER_PAIR_PENALTY_2=2.9431 PRIMER_LEFT_2_PENALTY=1.999797 PRIMER_RIGHT_2_PENALTY=0.943292 PRIMER_LEFT_2_SEQUENCE=CCAACCCAAAAATACAGACG PRIMER_RIGHT_2_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_2=8,20 PRIMER_RIGHT_2=148,20 PRIMER_LEFT_2_TM=58.000 PRIMER_RIGHT_2_TM=60.943 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_2=141 PRIMER_PAIR_PENALTY_3=2.9431 PRIMER_LEFT_3_PENALTY=1.999797 PRIMER_RIGHT_3_PENALTY=0.943292 PRIMER_LEFT_3_SEQUENCE=CAACCCAAAAATACAGACGG PRIMER_RIGHT_3_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_3=9,20 PRIMER_RIGHT_3=148,20 PRIMER_LEFT_3_TM=58.000 PRIMER_RIGHT_3_TM=60.943 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.6000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=5.00 PRIMER_PRODUCT_SIZE_3=140 PRIMER_PAIR_PENALTY_4=3.4069 PRIMER_LEFT_4_PENALTY=2.463612 PRIMER_RIGHT_4_PENALTY=0.943292 PRIMER_LEFT_4_SEQUENCE=CCAACCCAAAAATACAGACGG PRIMER_RIGHT_4_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_4=8,21 PRIMER_RIGHT_4=148,20 PRIMER_LEFT_4_TM=61.464 PRIMER_RIGHT_4_TM=60.943 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.6000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=5.00 PRIMER_PRODUCT_SIZE_4=141 PRIMER_PAIR_PENALTY_5=3.4702 PRIMER_LEFT_5_PENALTY=1.999797 PRIMER_RIGHT_5_PENALTY=1.470379 PRIMER_LEFT_5_SEQUENCE=CCAACCCAAAAATACAGACG PRIMER_RIGHT_5_SEQUENCE=CCCTGTGTTACCCGTCTCA PRIMER_LEFT_5=8,20 PRIMER_RIGHT_5=148,19 PRIMER_LEFT_5_TM=58.000 PRIMER_RIGHT_5_TM=59.530 PRIMER_LEFT_5_GC_PERCENT=45.000 PRIMER_RIGHT_5_GC_PERCENT=57.895 PRIMER_LEFT_5_SELF_ANY=2.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_LEFT_5_SELF_END=2.00 PRIMER_RIGHT_5_SELF_END=2.00 PRIMER_LEFT_5_END_STABILITY=8.1000 PRIMER_RIGHT_5_END_STABILITY=6.7000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_5=141 PRIMER_PAIR_PENALTY_6=3.4702 PRIMER_LEFT_6_PENALTY=1.999797 PRIMER_RIGHT_6_PENALTY=1.470379 PRIMER_LEFT_6_SEQUENCE=CAACCCAAAAATACAGACGG PRIMER_RIGHT_6_SEQUENCE=CCCTGTGTTACCCGTCTCA PRIMER_LEFT_6=9,20 PRIMER_RIGHT_6=148,19 PRIMER_LEFT_6_TM=58.000 PRIMER_RIGHT_6_TM=59.530 PRIMER_LEFT_6_GC_PERCENT=45.000 PRIMER_RIGHT_6_GC_PERCENT=57.895 PRIMER_LEFT_6_SELF_ANY=2.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_LEFT_6_SELF_END=1.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_END_STABILITY=9.6000 PRIMER_RIGHT_6_END_STABILITY=6.7000 PRIMER_PAIR_6_COMPL_ANY=6.00 PRIMER_PAIR_6_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_6=140 PRIMER_PAIR_PENALTY_7=3.8768 PRIMER_LEFT_7_PENALTY=2.933539 PRIMER_RIGHT_7_PENALTY=0.943292 PRIMER_LEFT_7_SEQUENCE=AACCCAAAAATACAGACGGA PRIMER_RIGHT_7_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_7=10,20 PRIMER_RIGHT_7=148,20 PRIMER_LEFT_7_TM=57.066 PRIMER_RIGHT_7_TM=60.943 PRIMER_LEFT_7_GC_PERCENT=40.000 PRIMER_RIGHT_7_GC_PERCENT=55.000 PRIMER_LEFT_7_SELF_ANY=2.00 PRIMER_RIGHT_7_SELF_ANY=3.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=9.6000 PRIMER_RIGHT_7_END_STABILITY=7.0000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_7=139 PRIMER_PAIR_PENALTY_8=3.9340 PRIMER_LEFT_8_PENALTY=2.463612 PRIMER_RIGHT_8_PENALTY=1.470379 PRIMER_LEFT_8_SEQUENCE=CCAACCCAAAAATACAGACGG PRIMER_RIGHT_8_SEQUENCE=CCCTGTGTTACCCGTCTCA PRIMER_LEFT_8=8,21 PRIMER_RIGHT_8=148,19 PRIMER_LEFT_8_TM=61.464 PRIMER_RIGHT_8_TM=59.530 PRIMER_LEFT_8_GC_PERCENT=47.619 PRIMER_RIGHT_8_GC_PERCENT=57.895 PRIMER_LEFT_8_SELF_ANY=2.00 PRIMER_RIGHT_8_SELF_ANY=3.00 PRIMER_LEFT_8_SELF_END=1.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_END_STABILITY=9.6000 PRIMER_RIGHT_8_END_STABILITY=6.7000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_8=141 PRIMER_PAIR_PENALTY_9=4.4039 PRIMER_LEFT_9_PENALTY=2.933539 PRIMER_RIGHT_9_PENALTY=1.470379 PRIMER_LEFT_9_SEQUENCE=AACCCAAAAATACAGACGGA PRIMER_RIGHT_9_SEQUENCE=CCCTGTGTTACCCGTCTCA PRIMER_LEFT_9=10,20 PRIMER_RIGHT_9=148,19 PRIMER_LEFT_9_TM=57.066 PRIMER_RIGHT_9_TM=59.530 PRIMER_LEFT_9_GC_PERCENT=40.000 PRIMER_RIGHT_9_GC_PERCENT=57.895 PRIMER_LEFT_9_SELF_ANY=2.00 PRIMER_RIGHT_9_SELF_ANY=3.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_END_STABILITY=9.6000 PRIMER_RIGHT_9_END_STABILITY=6.7000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_9=139 PRIMER_PAIR_PENALTY_10=4.4517 PRIMER_LEFT_10_PENALTY=4.436430 PRIMER_RIGHT_10_PENALTY=0.015253 PRIMER_LEFT_10_SEQUENCE=CACAAACAGAGACACAGACACAGA PRIMER_RIGHT_10_SEQUENCE=CTTCTGTGCTTTCCCGCTAC PRIMER_LEFT_10=36,24 PRIMER_RIGHT_10=210,20 PRIMER_LEFT_10_TM=60.436 PRIMER_RIGHT_10_TM=60.015 PRIMER_LEFT_10_GC_PERCENT=45.833 PRIMER_RIGHT_10_GC_PERCENT=55.000 PRIMER_LEFT_10_SELF_ANY=1.00 PRIMER_RIGHT_10_SELF_ANY=2.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_END_STABILITY=6.4000 PRIMER_RIGHT_10_END_STABILITY=6.9000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_10=175 PRIMER_PAIR_PENALTY_11=4.4977 PRIMER_LEFT_11_PENALTY=4.436430 PRIMER_RIGHT_11_PENALTY=0.061230 PRIMER_LEFT_11_SEQUENCE=CACAAACAGAGACACAGACACAGA PRIMER_RIGHT_11_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_11=36,24 PRIMER_RIGHT_11=203,20 PRIMER_LEFT_11_TM=60.436 PRIMER_RIGHT_11_TM=60.061 PRIMER_LEFT_11_GC_PERCENT=45.833 PRIMER_RIGHT_11_GC_PERCENT=55.000 PRIMER_LEFT_11_SELF_ANY=1.00 PRIMER_RIGHT_11_SELF_ANY=2.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_END_STABILITY=6.4000 PRIMER_RIGHT_11_END_STABILITY=5.9000 PRIMER_PAIR_11_COMPL_ANY=3.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_11=168 PRIMER_PAIR_PENALTY_12=4.5292 PRIMER_LEFT_12_PENALTY=4.513917 PRIMER_RIGHT_12_PENALTY=0.015253 PRIMER_LEFT_12_SEQUENCE=CACAAACAGAGACACAGACACAG PRIMER_RIGHT_12_SEQUENCE=CTTCTGTGCTTTCCCGCTAC PRIMER_LEFT_12=36,23 PRIMER_RIGHT_12=210,20 PRIMER_LEFT_12_TM=58.486 PRIMER_RIGHT_12_TM=60.015 PRIMER_LEFT_12_GC_PERCENT=47.826 PRIMER_RIGHT_12_GC_PERCENT=55.000 PRIMER_LEFT_12_SELF_ANY=1.00 PRIMER_RIGHT_12_SELF_ANY=2.00 PRIMER_LEFT_12_SELF_END=1.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_END_STABILITY=6.7000 PRIMER_RIGHT_12_END_STABILITY=6.9000 PRIMER_PAIR_12_COMPL_ANY=6.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_12=175 PRIMER_PAIR_PENALTY_13=4.5751 PRIMER_LEFT_13_PENALTY=4.513917 PRIMER_RIGHT_13_PENALTY=0.061230 PRIMER_LEFT_13_SEQUENCE=CACAAACAGAGACACAGACACAG PRIMER_RIGHT_13_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_13=36,23 PRIMER_RIGHT_13=203,20 PRIMER_LEFT_13_TM=58.486 PRIMER_RIGHT_13_TM=60.061 PRIMER_LEFT_13_GC_PERCENT=47.826 PRIMER_RIGHT_13_GC_PERCENT=55.000 PRIMER_LEFT_13_SELF_ANY=1.00 PRIMER_RIGHT_13_SELF_ANY=2.00 PRIMER_LEFT_13_SELF_END=1.00 PRIMER_RIGHT_13_SELF_END=0.00 PRIMER_LEFT_13_END_STABILITY=6.7000 PRIMER_RIGHT_13_END_STABILITY=5.9000 PRIMER_PAIR_13_COMPL_ANY=3.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_13=168 PRIMER_PAIR_PENALTY_14=4.6705 PRIMER_LEFT_14_PENALTY=4.609236 PRIMER_RIGHT_14_PENALTY=0.061230 PRIMER_LEFT_14_SEQUENCE=ACACAAACAGAGACACAGACACAG PRIMER_RIGHT_14_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_14=35,24 PRIMER_RIGHT_14=203,20 PRIMER_LEFT_14_TM=59.391 PRIMER_RIGHT_14_TM=60.061 PRIMER_LEFT_14_GC_PERCENT=45.833 PRIMER_RIGHT_14_GC_PERCENT=55.000 PRIMER_LEFT_14_SELF_ANY=1.00 PRIMER_RIGHT_14_SELF_ANY=2.00 PRIMER_LEFT_14_SELF_END=1.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=5.9000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_14=169 PRIMER_PAIR_PENALTY_15=4.6843 PRIMER_LEFT_15_PENALTY=4.669014 PRIMER_RIGHT_15_PENALTY=0.015253 PRIMER_LEFT_15_SEQUENCE=CACAAACAGAGACACAGACACA PRIMER_RIGHT_15_SEQUENCE=CTTCTGTGCTTTCCCGCTAC PRIMER_LEFT_15=36,22 PRIMER_RIGHT_15=210,20 PRIMER_LEFT_15_TM=57.331 PRIMER_RIGHT_15_TM=60.015 PRIMER_LEFT_15_GC_PERCENT=45.455 PRIMER_RIGHT_15_GC_PERCENT=55.000 PRIMER_LEFT_15_SELF_ANY=1.00 PRIMER_RIGHT_15_SELF_ANY=2.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_END_STABILITY=6.4000 PRIMER_RIGHT_15_END_STABILITY=6.9000 PRIMER_PAIR_15_COMPL_ANY=6.00 PRIMER_PAIR_15_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_15=175 PRIMER_PAIR_PENALTY_16=4.7302 PRIMER_LEFT_16_PENALTY=4.669014 PRIMER_RIGHT_16_PENALTY=0.061230 PRIMER_LEFT_16_SEQUENCE=CACAAACAGAGACACAGACACA PRIMER_RIGHT_16_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_16=36,22 PRIMER_RIGHT_16=203,20 PRIMER_LEFT_16_TM=57.331 PRIMER_RIGHT_16_TM=60.061 PRIMER_LEFT_16_GC_PERCENT=45.455 PRIMER_RIGHT_16_GC_PERCENT=55.000 PRIMER_LEFT_16_SELF_ANY=1.00 PRIMER_RIGHT_16_SELF_ANY=2.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_END_STABILITY=6.4000 PRIMER_RIGHT_16_END_STABILITY=5.9000 PRIMER_PAIR_16_COMPL_ANY=3.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_16=168 PRIMER_PAIR_PENALTY_17=4.7302 PRIMER_LEFT_17_PENALTY=4.669014 PRIMER_RIGHT_17_PENALTY=0.061230 PRIMER_LEFT_17_SEQUENCE=CACACAAACAGAGACACAGACA PRIMER_RIGHT_17_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_17=34,22 PRIMER_RIGHT_17=203,20 PRIMER_LEFT_17_TM=57.331 PRIMER_RIGHT_17_TM=60.061 PRIMER_LEFT_17_GC_PERCENT=45.455 PRIMER_RIGHT_17_GC_PERCENT=55.000 PRIMER_LEFT_17_SELF_ANY=1.00 PRIMER_RIGHT_17_SELF_ANY=2.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_END_STABILITY=6.4000 PRIMER_RIGHT_17_END_STABILITY=5.9000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_17=170 PRIMER_PAIR_PENALTY_18=4.7302 PRIMER_LEFT_18_PENALTY=4.669014 PRIMER_RIGHT_18_PENALTY=0.061230 PRIMER_LEFT_18_SEQUENCE=CACACACAAACAGAGACACAGA PRIMER_RIGHT_18_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_18=32,22 PRIMER_RIGHT_18=203,20 PRIMER_LEFT_18_TM=57.331 PRIMER_RIGHT_18_TM=60.061 PRIMER_LEFT_18_GC_PERCENT=45.455 PRIMER_RIGHT_18_GC_PERCENT=55.000 PRIMER_LEFT_18_SELF_ANY=1.00 PRIMER_RIGHT_18_SELF_ANY=2.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=0.00 PRIMER_LEFT_18_END_STABILITY=6.4000 PRIMER_RIGHT_18_END_STABILITY=5.9000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_18=172 PRIMER_PAIR_PENALTY_19=4.7308 PRIMER_LEFT_19_PENALTY=4.669525 PRIMER_RIGHT_19_PENALTY=0.061230 PRIMER_LEFT_19_SEQUENCE=CACACAAACAGAGACACAGACAC PRIMER_RIGHT_19_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_19=34,23 PRIMER_RIGHT_19=203,20 PRIMER_LEFT_19_TM=58.330 PRIMER_RIGHT_19_TM=60.061 PRIMER_LEFT_19_GC_PERCENT=47.826 PRIMER_RIGHT_19_GC_PERCENT=55.000 PRIMER_LEFT_19_SELF_ANY=1.00 PRIMER_RIGHT_19_SELF_ANY=2.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_END_STABILITY=6.1000 PRIMER_RIGHT_19_END_STABILITY=5.9000 PRIMER_PAIR_19_COMPL_ANY=3.00 PRIMER_PAIR_19_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_19=170 PRIMER_PAIR_PENALTY_20=4.7308 PRIMER_LEFT_20_PENALTY=4.669525 PRIMER_RIGHT_20_PENALTY=0.061230 PRIMER_LEFT_20_SEQUENCE=ACACAAACAGAGACACAGACACA PRIMER_RIGHT_20_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_20=35,23 PRIMER_RIGHT_20=203,20 PRIMER_LEFT_20_TM=58.330 PRIMER_RIGHT_20_TM=60.061 PRIMER_LEFT_20_GC_PERCENT=43.478 PRIMER_RIGHT_20_GC_PERCENT=55.000 PRIMER_LEFT_20_SELF_ANY=1.00 PRIMER_RIGHT_20_SELF_ANY=2.00 PRIMER_LEFT_20_SELF_END=0.00 PRIMER_RIGHT_20_SELF_END=0.00 PRIMER_LEFT_20_END_STABILITY=6.4000 PRIMER_RIGHT_20_END_STABILITY=5.9000 PRIMER_PAIR_20_COMPL_ANY=3.00 PRIMER_PAIR_20_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_20=169 PRIMER_PAIR_PENALTY_21=4.7308 PRIMER_LEFT_21_PENALTY=4.669525 PRIMER_RIGHT_21_PENALTY=0.061230 PRIMER_LEFT_21_SEQUENCE=ACACACAAACAGAGACACAGACA PRIMER_RIGHT_21_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_21=33,23 PRIMER_RIGHT_21=203,20 PRIMER_LEFT_21_TM=58.330 PRIMER_RIGHT_21_TM=60.061 PRIMER_LEFT_21_GC_PERCENT=43.478 PRIMER_RIGHT_21_GC_PERCENT=55.000 PRIMER_LEFT_21_SELF_ANY=1.00 PRIMER_RIGHT_21_SELF_ANY=2.00 PRIMER_LEFT_21_SELF_END=0.00 PRIMER_RIGHT_21_SELF_END=0.00 PRIMER_LEFT_21_END_STABILITY=6.4000 PRIMER_RIGHT_21_END_STABILITY=5.9000 PRIMER_PAIR_21_COMPL_ANY=3.00 PRIMER_PAIR_21_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_21=171 PRIMER_PAIR_PENALTY_22=4.7308 PRIMER_LEFT_22_PENALTY=4.669525 PRIMER_RIGHT_22_PENALTY=0.061230 PRIMER_LEFT_22_SEQUENCE=CACACACAAACAGAGACACAGAC PRIMER_RIGHT_22_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_22=32,23 PRIMER_RIGHT_22=203,20 PRIMER_LEFT_22_TM=58.330 PRIMER_RIGHT_22_TM=60.061 PRIMER_LEFT_22_GC_PERCENT=47.826 PRIMER_RIGHT_22_GC_PERCENT=55.000 PRIMER_LEFT_22_SELF_ANY=1.00 PRIMER_RIGHT_22_SELF_ANY=2.00 PRIMER_LEFT_22_SELF_END=1.00 PRIMER_RIGHT_22_SELF_END=0.00 PRIMER_LEFT_22_END_STABILITY=6.4000 PRIMER_RIGHT_22_END_STABILITY=5.9000 PRIMER_PAIR_22_COMPL_ANY=3.00 PRIMER_PAIR_22_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_22=172 PRIMER_PAIR_PENALTY_23=4.7308 PRIMER_LEFT_23_PENALTY=4.669525 PRIMER_RIGHT_23_PENALTY=0.061230 PRIMER_LEFT_23_SEQUENCE=ACACACACAAACAGAGACACAGA PRIMER_RIGHT_23_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_23=31,23 PRIMER_RIGHT_23=203,20 PRIMER_LEFT_23_TM=58.330 PRIMER_RIGHT_23_TM=60.061 PRIMER_LEFT_23_GC_PERCENT=43.478 PRIMER_RIGHT_23_GC_PERCENT=55.000 PRIMER_LEFT_23_SELF_ANY=1.00 PRIMER_RIGHT_23_SELF_ANY=2.00 PRIMER_LEFT_23_SELF_END=0.00 PRIMER_RIGHT_23_SELF_END=0.00 PRIMER_LEFT_23_END_STABILITY=6.4000 PRIMER_RIGHT_23_END_STABILITY=5.9000 PRIMER_PAIR_23_COMPL_ANY=3.00 PRIMER_PAIR_23_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_23=173 PRIMER_PAIR_PENALTY_24=4.8123 PRIMER_LEFT_24_PENALTY=4.751110 PRIMER_RIGHT_24_PENALTY=0.061230 PRIMER_LEFT_24_SEQUENCE=ACACACAAACAGAGACACAGACAC PRIMER_RIGHT_24_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_24=33,24 PRIMER_RIGHT_24=203,20 PRIMER_LEFT_24_TM=59.249 PRIMER_RIGHT_24_TM=60.061 PRIMER_LEFT_24_GC_PERCENT=45.833 PRIMER_RIGHT_24_GC_PERCENT=55.000 PRIMER_LEFT_24_SELF_ANY=1.00 PRIMER_RIGHT_24_SELF_ANY=2.00 PRIMER_LEFT_24_SELF_END=0.00 PRIMER_RIGHT_24_SELF_END=0.00 PRIMER_LEFT_24_END_STABILITY=6.1000 PRIMER_RIGHT_24_END_STABILITY=5.9000 PRIMER_PAIR_24_COMPL_ANY=3.00 PRIMER_PAIR_24_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_24=171 PRIMER_PAIR_PENALTY_25=4.8123 PRIMER_LEFT_25_PENALTY=4.751110 PRIMER_RIGHT_25_PENALTY=0.061230 PRIMER_LEFT_25_SEQUENCE=ACACACACAAACAGAGACACAGAC PRIMER_RIGHT_25_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_25=31,24 PRIMER_RIGHT_25=203,20 PRIMER_LEFT_25_TM=59.249 PRIMER_RIGHT_25_TM=60.061 PRIMER_LEFT_25_GC_PERCENT=45.833 PRIMER_RIGHT_25_GC_PERCENT=55.000 PRIMER_LEFT_25_SELF_ANY=1.00 PRIMER_RIGHT_25_SELF_ANY=2.00 PRIMER_LEFT_25_SELF_END=1.00 PRIMER_RIGHT_25_SELF_END=0.00 PRIMER_LEFT_25_END_STABILITY=6.4000 PRIMER_RIGHT_25_END_STABILITY=5.9000 PRIMER_PAIR_25_COMPL_ANY=3.00 PRIMER_PAIR_25_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_25=173 PRIMER_PAIR_PENALTY_26=4.9077 PRIMER_LEFT_26_PENALTY=4.436430 PRIMER_RIGHT_26_PENALTY=0.471231 PRIMER_LEFT_26_SEQUENCE=CACAAACAGAGACACAGACACAGA PRIMER_RIGHT_26_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_26=36,24 PRIMER_RIGHT_26=205,20 PRIMER_LEFT_26_TM=60.436 PRIMER_RIGHT_26_TM=60.471 PRIMER_LEFT_26_GC_PERCENT=45.833 PRIMER_RIGHT_26_GC_PERCENT=55.000 PRIMER_LEFT_26_SELF_ANY=1.00 PRIMER_RIGHT_26_SELF_ANY=2.00 PRIMER_LEFT_26_SELF_END=0.00 PRIMER_RIGHT_26_SELF_END=2.00 PRIMER_LEFT_26_END_STABILITY=6.4000 PRIMER_RIGHT_26_END_STABILITY=7.1000 PRIMER_PAIR_26_COMPL_ANY=3.00 PRIMER_PAIR_26_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_26=170 PRIMER_PAIR_PENALTY_27=4.9851 PRIMER_LEFT_27_PENALTY=4.513917 PRIMER_RIGHT_27_PENALTY=0.471231 PRIMER_LEFT_27_SEQUENCE=CACAAACAGAGACACAGACACAG PRIMER_RIGHT_27_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_27=36,23 PRIMER_RIGHT_27=205,20 PRIMER_LEFT_27_TM=58.486 PRIMER_RIGHT_27_TM=60.471 PRIMER_LEFT_27_GC_PERCENT=47.826 PRIMER_RIGHT_27_GC_PERCENT=55.000 PRIMER_LEFT_27_SELF_ANY=1.00 PRIMER_RIGHT_27_SELF_ANY=2.00 PRIMER_LEFT_27_SELF_END=1.00 PRIMER_RIGHT_27_SELF_END=2.00 PRIMER_LEFT_27_END_STABILITY=6.7000 PRIMER_RIGHT_27_END_STABILITY=7.1000 PRIMER_PAIR_27_COMPL_ANY=3.00 PRIMER_PAIR_27_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_27=170 PRIMER_PAIR_PENALTY_28=5.0805 PRIMER_LEFT_28_PENALTY=4.609236 PRIMER_RIGHT_28_PENALTY=0.471231 PRIMER_LEFT_28_SEQUENCE=ACACAAACAGAGACACAGACACAG PRIMER_RIGHT_28_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_28=35,24 PRIMER_RIGHT_28=205,20 PRIMER_LEFT_28_TM=59.391 PRIMER_RIGHT_28_TM=60.471 PRIMER_LEFT_28_GC_PERCENT=45.833 PRIMER_RIGHT_28_GC_PERCENT=55.000 PRIMER_LEFT_28_SELF_ANY=1.00 PRIMER_RIGHT_28_SELF_ANY=2.00 PRIMER_LEFT_28_SELF_END=1.00 PRIMER_RIGHT_28_SELF_END=2.00 PRIMER_LEFT_28_END_STABILITY=6.7000 PRIMER_RIGHT_28_END_STABILITY=7.1000 PRIMER_PAIR_28_COMPL_ANY=3.00 PRIMER_PAIR_28_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_28=171 PRIMER_PAIR_PENALTY_29=5.0958 PRIMER_LEFT_29_PENALTY=1.153194 PRIMER_RIGHT_29_PENALTY=3.942558 PRIMER_LEFT_29_SEQUENCE=CAACCCAAAAATACAGACGGA PRIMER_RIGHT_29_SEQUENCE=CCTGTGTTACCCGTCTCAA PRIMER_LEFT_29=9,21 PRIMER_RIGHT_29=147,19 PRIMER_LEFT_29_TM=59.847 PRIMER_RIGHT_29_TM=57.057 PRIMER_LEFT_29_GC_PERCENT=42.857 PRIMER_RIGHT_29_GC_PERCENT=52.632 PRIMER_LEFT_29_SELF_ANY=2.00 PRIMER_RIGHT_29_SELF_ANY=3.00 PRIMER_LEFT_29_SELF_END=0.00 PRIMER_RIGHT_29_SELF_END=0.00 PRIMER_LEFT_29_END_STABILITY=9.6000 PRIMER_RIGHT_29_END_STABILITY=7.0000 PRIMER_PAIR_29_COMPL_ANY=6.00 PRIMER_PAIR_29_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_29=139 PRIMER_PAIR_PENALTY_30=5.1402 PRIMER_LEFT_30_PENALTY=4.669014 PRIMER_RIGHT_30_PENALTY=0.471231 PRIMER_LEFT_30_SEQUENCE=CACAAACAGAGACACAGACACA PRIMER_RIGHT_30_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_30=36,22 PRIMER_RIGHT_30=205,20 PRIMER_LEFT_30_TM=57.331 PRIMER_RIGHT_30_TM=60.471 PRIMER_LEFT_30_GC_PERCENT=45.455 PRIMER_RIGHT_30_GC_PERCENT=55.000 PRIMER_LEFT_30_SELF_ANY=1.00 PRIMER_RIGHT_30_SELF_ANY=2.00 PRIMER_LEFT_30_SELF_END=0.00 PRIMER_RIGHT_30_SELF_END=2.00 PRIMER_LEFT_30_END_STABILITY=6.4000 PRIMER_RIGHT_30_END_STABILITY=7.1000 PRIMER_PAIR_30_COMPL_ANY=3.00 PRIMER_PAIR_30_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_30=170 PRIMER_PAIR_PENALTY_31=5.1402 PRIMER_LEFT_31_PENALTY=4.669014 PRIMER_RIGHT_31_PENALTY=0.471231 PRIMER_LEFT_31_SEQUENCE=CACACAAACAGAGACACAGACA PRIMER_RIGHT_31_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_31=34,22 PRIMER_RIGHT_31=205,20 PRIMER_LEFT_31_TM=57.331 PRIMER_RIGHT_31_TM=60.471 PRIMER_LEFT_31_GC_PERCENT=45.455 PRIMER_RIGHT_31_GC_PERCENT=55.000 PRIMER_LEFT_31_SELF_ANY=1.00 PRIMER_RIGHT_31_SELF_ANY=2.00 PRIMER_LEFT_31_SELF_END=0.00 PRIMER_RIGHT_31_SELF_END=2.00 PRIMER_LEFT_31_END_STABILITY=6.4000 PRIMER_RIGHT_31_END_STABILITY=7.1000 PRIMER_PAIR_31_COMPL_ANY=3.00 PRIMER_PAIR_31_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_31=172 PRIMER_PAIR_PENALTY_32=5.1402 PRIMER_LEFT_32_PENALTY=4.669014 PRIMER_RIGHT_32_PENALTY=0.471231 PRIMER_LEFT_32_SEQUENCE=CACACACAAACAGAGACACAGA PRIMER_RIGHT_32_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_32=32,22 PRIMER_RIGHT_32=205,20 PRIMER_LEFT_32_TM=57.331 PRIMER_RIGHT_32_TM=60.471 PRIMER_LEFT_32_GC_PERCENT=45.455 PRIMER_RIGHT_32_GC_PERCENT=55.000 PRIMER_LEFT_32_SELF_ANY=1.00 PRIMER_RIGHT_32_SELF_ANY=2.00 PRIMER_LEFT_32_SELF_END=0.00 PRIMER_RIGHT_32_SELF_END=2.00 PRIMER_LEFT_32_END_STABILITY=6.4000 PRIMER_RIGHT_32_END_STABILITY=7.1000 PRIMER_PAIR_32_COMPL_ANY=3.00 PRIMER_PAIR_32_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_32=174 PRIMER_PAIR_PENALTY_33=5.1408 PRIMER_LEFT_33_PENALTY=4.669525 PRIMER_RIGHT_33_PENALTY=0.471231 PRIMER_LEFT_33_SEQUENCE=CACACAAACAGAGACACAGACAC PRIMER_RIGHT_33_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_33=34,23 PRIMER_RIGHT_33=205,20 PRIMER_LEFT_33_TM=58.330 PRIMER_RIGHT_33_TM=60.471 PRIMER_LEFT_33_GC_PERCENT=47.826 PRIMER_RIGHT_33_GC_PERCENT=55.000 PRIMER_LEFT_33_SELF_ANY=1.00 PRIMER_RIGHT_33_SELF_ANY=2.00 PRIMER_LEFT_33_SELF_END=0.00 PRIMER_RIGHT_33_SELF_END=2.00 PRIMER_LEFT_33_END_STABILITY=6.1000 PRIMER_RIGHT_33_END_STABILITY=7.1000 PRIMER_PAIR_33_COMPL_ANY=3.00 PRIMER_PAIR_33_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_33=172 PRIMER_PAIR_PENALTY_34=5.1408 PRIMER_LEFT_34_PENALTY=4.669525 PRIMER_RIGHT_34_PENALTY=0.471231 PRIMER_LEFT_34_SEQUENCE=ACACAAACAGAGACACAGACACA PRIMER_RIGHT_34_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_34=35,23 PRIMER_RIGHT_34=205,20 PRIMER_LEFT_34_TM=58.330 PRIMER_RIGHT_34_TM=60.471 PRIMER_LEFT_34_GC_PERCENT=43.478 PRIMER_RIGHT_34_GC_PERCENT=55.000 PRIMER_LEFT_34_SELF_ANY=1.00 PRIMER_RIGHT_34_SELF_ANY=2.00 PRIMER_LEFT_34_SELF_END=0.00 PRIMER_RIGHT_34_SELF_END=2.00 PRIMER_LEFT_34_END_STABILITY=6.4000 PRIMER_RIGHT_34_END_STABILITY=7.1000 PRIMER_PAIR_34_COMPL_ANY=3.00 PRIMER_PAIR_34_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_34=171 PRIMER_PAIR_PENALTY_35=5.1408 PRIMER_LEFT_35_PENALTY=4.669525 PRIMER_RIGHT_35_PENALTY=0.471231 PRIMER_LEFT_35_SEQUENCE=ACACACAAACAGAGACACAGACA PRIMER_RIGHT_35_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_35=33,23 PRIMER_RIGHT_35=205,20 PRIMER_LEFT_35_TM=58.330 PRIMER_RIGHT_35_TM=60.471 PRIMER_LEFT_35_GC_PERCENT=43.478 PRIMER_RIGHT_35_GC_PERCENT=55.000 PRIMER_LEFT_35_SELF_ANY=1.00 PRIMER_RIGHT_35_SELF_ANY=2.00 PRIMER_LEFT_35_SELF_END=0.00 PRIMER_RIGHT_35_SELF_END=2.00 PRIMER_LEFT_35_END_STABILITY=6.4000 PRIMER_RIGHT_35_END_STABILITY=7.1000 PRIMER_PAIR_35_COMPL_ANY=3.00 PRIMER_PAIR_35_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_35=173 PRIMER_PAIR_PENALTY_36=5.1408 PRIMER_LEFT_36_PENALTY=4.669525 PRIMER_RIGHT_36_PENALTY=0.471231 PRIMER_LEFT_36_SEQUENCE=CACACACAAACAGAGACACAGAC PRIMER_RIGHT_36_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_36=32,23 PRIMER_RIGHT_36=205,20 PRIMER_LEFT_36_TM=58.330 PRIMER_RIGHT_36_TM=60.471 PRIMER_LEFT_36_GC_PERCENT=47.826 PRIMER_RIGHT_36_GC_PERCENT=55.000 PRIMER_LEFT_36_SELF_ANY=1.00 PRIMER_RIGHT_36_SELF_ANY=2.00 PRIMER_LEFT_36_SELF_END=1.00 PRIMER_RIGHT_36_SELF_END=2.00 PRIMER_LEFT_36_END_STABILITY=6.4000 PRIMER_RIGHT_36_END_STABILITY=7.1000 PRIMER_PAIR_36_COMPL_ANY=3.00 PRIMER_PAIR_36_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_36=174 PRIMER_PAIR_PENALTY_37=5.1408 PRIMER_LEFT_37_PENALTY=4.669525 PRIMER_RIGHT_37_PENALTY=0.471231 PRIMER_LEFT_37_SEQUENCE=ACACACACAAACAGAGACACAGA PRIMER_RIGHT_37_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_37=31,23 PRIMER_RIGHT_37=205,20 PRIMER_LEFT_37_TM=58.330 PRIMER_RIGHT_37_TM=60.471 PRIMER_LEFT_37_GC_PERCENT=43.478 PRIMER_RIGHT_37_GC_PERCENT=55.000 PRIMER_LEFT_37_SELF_ANY=1.00 PRIMER_RIGHT_37_SELF_ANY=2.00 PRIMER_LEFT_37_SELF_END=0.00 PRIMER_RIGHT_37_SELF_END=2.00 PRIMER_LEFT_37_END_STABILITY=6.4000 PRIMER_RIGHT_37_END_STABILITY=7.1000 PRIMER_PAIR_37_COMPL_ANY=3.00 PRIMER_PAIR_37_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_37=175 PRIMER_PAIR_PENALTY_38=5.2223 PRIMER_LEFT_38_PENALTY=4.751110 PRIMER_RIGHT_38_PENALTY=0.471231 PRIMER_LEFT_38_SEQUENCE=ACACACAAACAGAGACACAGACAC PRIMER_RIGHT_38_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_38=33,24 PRIMER_RIGHT_38=205,20 PRIMER_LEFT_38_TM=59.249 PRIMER_RIGHT_38_TM=60.471 PRIMER_LEFT_38_GC_PERCENT=45.833 PRIMER_RIGHT_38_GC_PERCENT=55.000 PRIMER_LEFT_38_SELF_ANY=1.00 PRIMER_RIGHT_38_SELF_ANY=2.00 PRIMER_LEFT_38_SELF_END=0.00 PRIMER_RIGHT_38_SELF_END=2.00 PRIMER_LEFT_38_END_STABILITY=6.1000 PRIMER_RIGHT_38_END_STABILITY=7.1000 PRIMER_PAIR_38_COMPL_ANY=3.00 PRIMER_PAIR_38_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_38=173 PRIMER_PAIR_PENALTY_39=5.2223 PRIMER_LEFT_39_PENALTY=4.751110 PRIMER_RIGHT_39_PENALTY=0.471231 PRIMER_LEFT_39_SEQUENCE=ACACACACAAACAGAGACACAGAC PRIMER_RIGHT_39_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_39=31,24 PRIMER_RIGHT_39=205,20 PRIMER_LEFT_39_TM=59.249 PRIMER_RIGHT_39_TM=60.471 PRIMER_LEFT_39_GC_PERCENT=45.833 PRIMER_RIGHT_39_GC_PERCENT=55.000 PRIMER_LEFT_39_SELF_ANY=1.00 PRIMER_RIGHT_39_SELF_ANY=2.00 PRIMER_LEFT_39_SELF_END=1.00 PRIMER_RIGHT_39_SELF_END=2.00 PRIMER_LEFT_39_END_STABILITY=6.4000 PRIMER_RIGHT_39_END_STABILITY=7.1000 PRIMER_PAIR_39_COMPL_ANY=3.00 PRIMER_PAIR_39_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_39=175 = MARKER_NAME=07.RHAP34FE7.seq SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT INCLUDED_REGION=18,283 TARGET=115,29,CT TARGET=145,20,NG TARGET=166,24,CA PRIMER_PAIR_PENALTY=0.4846 PRIMER_LEFT_PENALTY=0.151542 PRIMER_RIGHT_PENALTY=0.333053 PRIMER_LEFT_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT=76,20 PRIMER_RIGHT=238,20 PRIMER_LEFT_TM=60.152 PRIMER_RIGHT_TM=60.333 PRIMER_LEFT_GC_PERCENT=50.000 PRIMER_RIGHT_GC_PERCENT=50.000 PRIMER_LEFT_SELF_ANY=5.00 PRIMER_RIGHT_SELF_ANY=6.00 PRIMER_LEFT_SELF_END=3.00 PRIMER_RIGHT_SELF_END=0.00 PRIMER_LEFT_END_STABILITY=9.7000 PRIMER_RIGHT_END_STABILITY=7.9000 PRIMER_PAIR_MAX_COMPL_ANY=4.00 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_PRODUCT_SIZE=163 PRIMER_PAIR_PENALTY_1=0.6812 PRIMER_LEFT_1_PENALTY=0.151542 PRIMER_RIGHT_1_PENALTY=0.529649 PRIMER_LEFT_1_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_1_SEQUENCE=GCACGCATGTAGAGGTTCAG PRIMER_LEFT_1=76,20 PRIMER_RIGHT_1=235,20 PRIMER_LEFT_1_TM=60.152 PRIMER_RIGHT_1_TM=59.470 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_1=160 PRIMER_PAIR_PENALTY_2=0.7897 PRIMER_LEFT_2_PENALTY=0.151542 PRIMER_RIGHT_2_PENALTY=0.638191 PRIMER_LEFT_2_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_2_SEQUENCE=AGAGGTTCAGGTGGAGAGGG PRIMER_LEFT_2=76,20 PRIMER_RIGHT_2=225,20 PRIMER_LEFT_2_TM=60.152 PRIMER_RIGHT_2_TM=60.638 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_2=150 PRIMER_PAIR_PENALTY_3=0.8285 PRIMER_LEFT_3_PENALTY=0.151542 PRIMER_RIGHT_3_PENALTY=0.676947 PRIMER_LEFT_3_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_3_SEQUENCE=GTGCACGCATGTAGAGGTTC PRIMER_LEFT_3=76,20 PRIMER_RIGHT_3=237,20 PRIMER_LEFT_3_TM=60.152 PRIMER_RIGHT_3_TM=59.323 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_3=162 PRIMER_PAIR_PENALTY_4=0.8378 PRIMER_LEFT_4_PENALTY=0.151542 PRIMER_RIGHT_4_PENALTY=0.686256 PRIMER_LEFT_4_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_4_SEQUENCE=CACGCATGTAGAGGTTCAGG PRIMER_LEFT_4=76,20 PRIMER_RIGHT_4=234,20 PRIMER_LEFT_4_TM=60.152 PRIMER_RIGHT_4_TM=59.314 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_4=159 PRIMER_PAIR_PENALTY_5=0.8378 PRIMER_LEFT_5_PENALTY=0.151542 PRIMER_RIGHT_5_PENALTY=0.686256 PRIMER_LEFT_5_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_5_SEQUENCE=CGCATGTAGAGGTTCAGGTG PRIMER_LEFT_5=76,20 PRIMER_RIGHT_5=232,20 PRIMER_LEFT_5_TM=60.152 PRIMER_RIGHT_5_TM=59.314 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=55.000 PRIMER_LEFT_5_SELF_ANY=5.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=2.00 PRIMER_LEFT_5_END_STABILITY=9.7000 PRIMER_RIGHT_5_END_STABILITY=7.9000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_5=157 PRIMER_PAIR_PENALTY_6=0.9180 PRIMER_LEFT_6_PENALTY=0.151542 PRIMER_RIGHT_6_PENALTY=0.766499 PRIMER_LEFT_6_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_6_SEQUENCE=TGAACATGTGCACGCATGTA PRIMER_LEFT_6=76,20 PRIMER_RIGHT_6=244,20 PRIMER_LEFT_6_TM=60.152 PRIMER_RIGHT_6_TM=60.766 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=45.000 PRIMER_LEFT_6_SELF_ANY=5.00 PRIMER_RIGHT_6_SELF_ANY=8.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=6.00 PRIMER_LEFT_6_END_STABILITY=9.7000 PRIMER_RIGHT_6_END_STABILITY=5.6000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_6=169 PRIMER_PAIR_PENALTY_7=0.9562 PRIMER_LEFT_7_PENALTY=0.151542 PRIMER_RIGHT_7_PENALTY=0.804687 PRIMER_LEFT_7_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_7_SEQUENCE=ATGTGCACGCATGTAGAGGT PRIMER_LEFT_7=76,20 PRIMER_RIGHT_7=239,20 PRIMER_LEFT_7_TM=60.152 PRIMER_RIGHT_7_TM=59.195 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=50.000 PRIMER_LEFT_7_SELF_ANY=5.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=3.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=7.6000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_7=164 PRIMER_PAIR_PENALTY_8=1.0167 PRIMER_LEFT_8_PENALTY=0.683672 PRIMER_RIGHT_8_PENALTY=0.333053 PRIMER_LEFT_8_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_8_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_8=88,20 PRIMER_RIGHT_8=238,20 PRIMER_LEFT_8_TM=59.316 PRIMER_RIGHT_8_TM=60.333 PRIMER_LEFT_8_GC_PERCENT=45.000 PRIMER_RIGHT_8_GC_PERCENT=50.000 PRIMER_LEFT_8_SELF_ANY=5.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=5.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_LEFT_8_END_STABILITY=9.7000 PRIMER_RIGHT_8_END_STABILITY=7.9000 PRIMER_PAIR_8_COMPL_ANY=4.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_8=151 PRIMER_PAIR_PENALTY_9=1.1088 PRIMER_LEFT_9_PENALTY=0.775718 PRIMER_RIGHT_9_PENALTY=0.333053 PRIMER_LEFT_9_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_9_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_9=86,20 PRIMER_RIGHT_9=238,20 PRIMER_LEFT_9_TM=60.776 PRIMER_RIGHT_9_TM=60.333 PRIMER_LEFT_9_GC_PERCENT=50.000 PRIMER_RIGHT_9_GC_PERCENT=50.000 PRIMER_LEFT_9_SELF_ANY=5.00 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=0.00 PRIMER_LEFT_9_END_STABILITY=9.7000 PRIMER_RIGHT_9_END_STABILITY=7.9000 PRIMER_PAIR_9_COMPL_ANY=4.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_9=153 PRIMER_PAIR_PENALTY_10=1.1088 PRIMER_LEFT_10_PENALTY=0.775718 PRIMER_RIGHT_10_PENALTY=0.333053 PRIMER_LEFT_10_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_10_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_10=87,20 PRIMER_RIGHT_10=238,20 PRIMER_LEFT_10_TM=60.776 PRIMER_RIGHT_10_TM=60.333 PRIMER_LEFT_10_GC_PERCENT=45.000 PRIMER_RIGHT_10_GC_PERCENT=50.000 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_END_STABILITY=9.4000 PRIMER_RIGHT_10_END_STABILITY=7.9000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_10=152 PRIMER_PAIR_PENALTY_11=1.2133 PRIMER_LEFT_11_PENALTY=0.683672 PRIMER_RIGHT_11_PENALTY=0.529649 PRIMER_LEFT_11_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_11_SEQUENCE=GCACGCATGTAGAGGTTCAG PRIMER_LEFT_11=88,20 PRIMER_RIGHT_11=235,20 PRIMER_LEFT_11_TM=59.316 PRIMER_RIGHT_11_TM=59.470 PRIMER_LEFT_11_GC_PERCENT=45.000 PRIMER_RIGHT_11_GC_PERCENT=55.000 PRIMER_LEFT_11_SELF_ANY=5.00 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=5.00 PRIMER_RIGHT_11_SELF_END=1.00 PRIMER_LEFT_11_END_STABILITY=9.7000 PRIMER_RIGHT_11_END_STABILITY=7.0000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_11=148 PRIMER_PAIR_PENALTY_12=1.2778 PRIMER_LEFT_12_PENALTY=0.151542 PRIMER_RIGHT_12_PENALTY=1.126283 PRIMER_LEFT_12_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_12_SEQUENCE=GCATGTAGAGGTTCAGGTGGA PRIMER_LEFT_12=76,20 PRIMER_RIGHT_12=231,21 PRIMER_LEFT_12_TM=60.152 PRIMER_RIGHT_12_TM=60.126 PRIMER_LEFT_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_GC_PERCENT=52.381 PRIMER_LEFT_12_SELF_ANY=5.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=3.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_END_STABILITY=9.7000 PRIMER_RIGHT_12_END_STABILITY=7.9000 PRIMER_PAIR_12_COMPL_ANY=4.00 PRIMER_PAIR_12_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_12=156 PRIMER_PAIR_PENALTY_13=1.3054 PRIMER_LEFT_13_PENALTY=0.775718 PRIMER_RIGHT_13_PENALTY=0.529649 PRIMER_LEFT_13_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_13_SEQUENCE=GCACGCATGTAGAGGTTCAG PRIMER_LEFT_13=86,20 PRIMER_RIGHT_13=235,20 PRIMER_LEFT_13_TM=60.776 PRIMER_RIGHT_13_TM=59.470 PRIMER_LEFT_13_GC_PERCENT=50.000 PRIMER_RIGHT_13_GC_PERCENT=55.000 PRIMER_LEFT_13_SELF_ANY=5.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_LEFT_13_END_STABILITY=9.7000 PRIMER_RIGHT_13_END_STABILITY=7.0000 PRIMER_PAIR_13_COMPL_ANY=4.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_13=150 PRIMER_PAIR_PENALTY_14=1.3054 PRIMER_LEFT_14_PENALTY=0.775718 PRIMER_RIGHT_14_PENALTY=0.529649 PRIMER_LEFT_14_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_14_SEQUENCE=GCACGCATGTAGAGGTTCAG PRIMER_LEFT_14=87,20 PRIMER_RIGHT_14=235,20 PRIMER_LEFT_14_TM=60.776 PRIMER_RIGHT_14_TM=59.470 PRIMER_LEFT_14_GC_PERCENT=45.000 PRIMER_RIGHT_14_GC_PERCENT=55.000 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=4.00 PRIMER_LEFT_14_SELF_END=3.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_END_STABILITY=9.4000 PRIMER_RIGHT_14_END_STABILITY=7.0000 PRIMER_PAIR_14_COMPL_ANY=4.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_14=149 PRIMER_PAIR_PENALTY_15=1.3219 PRIMER_LEFT_15_PENALTY=0.683672 PRIMER_RIGHT_15_PENALTY=0.638191 PRIMER_LEFT_15_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_15_SEQUENCE=AGAGGTTCAGGTGGAGAGGG PRIMER_LEFT_15=88,20 PRIMER_RIGHT_15=225,20 PRIMER_LEFT_15_TM=59.316 PRIMER_RIGHT_15_TM=60.638 PRIMER_LEFT_15_GC_PERCENT=45.000 PRIMER_RIGHT_15_GC_PERCENT=60.000 PRIMER_LEFT_15_SELF_ANY=5.00 PRIMER_RIGHT_15_SELF_ANY=2.00 PRIMER_LEFT_15_SELF_END=5.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_END_STABILITY=9.7000 PRIMER_RIGHT_15_END_STABILITY=9.4000 PRIMER_PAIR_15_COMPL_ANY=2.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_15=138 PRIMER_PAIR_PENALTY_16=1.3375 PRIMER_LEFT_16_PENALTY=0.151542 PRIMER_RIGHT_16_PENALTY=1.185942 PRIMER_LEFT_16_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_16_SEQUENCE=CACGCATGTAGAGGTTCAGGT PRIMER_LEFT_16=76,20 PRIMER_RIGHT_16=234,21 PRIMER_LEFT_16_TM=60.152 PRIMER_RIGHT_16_TM=60.186 PRIMER_LEFT_16_GC_PERCENT=50.000 PRIMER_RIGHT_16_GC_PERCENT=52.381 PRIMER_LEFT_16_SELF_ANY=5.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_END_STABILITY=9.7000 PRIMER_RIGHT_16_END_STABILITY=7.9000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_16=159 PRIMER_PAIR_PENALTY_17=1.3375 PRIMER_LEFT_17_PENALTY=0.151542 PRIMER_RIGHT_17_PENALTY=1.185942 PRIMER_LEFT_17_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_17_SEQUENCE=ACGCATGTAGAGGTTCAGGTG PRIMER_LEFT_17=76,20 PRIMER_RIGHT_17=233,21 PRIMER_LEFT_17_TM=60.152 PRIMER_RIGHT_17_TM=60.186 PRIMER_LEFT_17_GC_PERCENT=50.000 PRIMER_RIGHT_17_GC_PERCENT=52.381 PRIMER_LEFT_17_SELF_ANY=5.00 PRIMER_RIGHT_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_END_STABILITY=9.7000 PRIMER_RIGHT_17_END_STABILITY=7.9000 PRIMER_PAIR_17_COMPL_ANY=4.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_17=158 PRIMER_PAIR_PENALTY_18=1.3484 PRIMER_LEFT_18_PENALTY=0.151542 PRIMER_RIGHT_18_PENALTY=1.196903 PRIMER_LEFT_18_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_18_SEQUENCE=AACATGTGCACGCATGTAGAG PRIMER_LEFT_18=76,20 PRIMER_RIGHT_18=242,21 PRIMER_LEFT_18_TM=60.152 PRIMER_RIGHT_18_TM=59.803 PRIMER_LEFT_18_GC_PERCENT=50.000 PRIMER_RIGHT_18_GC_PERCENT=47.619 PRIMER_LEFT_18_SELF_ANY=5.00 PRIMER_RIGHT_18_SELF_ANY=8.00 PRIMER_LEFT_18_SELF_END=3.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_END_STABILITY=9.7000 PRIMER_RIGHT_18_END_STABILITY=5.7000 PRIMER_PAIR_18_COMPL_ANY=4.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_18=167 PRIMER_PAIR_PENALTY_19=1.3558 PRIMER_LEFT_19_PENALTY=1.022788 PRIMER_RIGHT_19_PENALTY=0.333053 PRIMER_LEFT_19_SEQUENCE=TTTGGGTACAAACTGACAGGC PRIMER_RIGHT_19_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_19=74,21 PRIMER_RIGHT_19=238,20 PRIMER_LEFT_19_TM=60.023 PRIMER_RIGHT_19_TM=60.333 PRIMER_LEFT_19_GC_PERCENT=47.619 PRIMER_RIGHT_19_GC_PERCENT=50.000 PRIMER_LEFT_19_SELF_ANY=5.00 PRIMER_RIGHT_19_SELF_ANY=6.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_END_STABILITY=9.7000 PRIMER_RIGHT_19_END_STABILITY=7.9000 PRIMER_PAIR_19_COMPL_ANY=4.00 PRIMER_PAIR_19_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_19=165 PRIMER_PAIR_PENALTY_20=1.3606 PRIMER_LEFT_20_PENALTY=0.683672 PRIMER_RIGHT_20_PENALTY=0.676947 PRIMER_LEFT_20_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_20_SEQUENCE=GTGCACGCATGTAGAGGTTC PRIMER_LEFT_20=88,20 PRIMER_RIGHT_20=237,20 PRIMER_LEFT_20_TM=59.316 PRIMER_RIGHT_20_TM=59.323 PRIMER_LEFT_20_GC_PERCENT=45.000 PRIMER_RIGHT_20_GC_PERCENT=55.000 PRIMER_LEFT_20_SELF_ANY=5.00 PRIMER_RIGHT_20_SELF_ANY=6.00 PRIMER_LEFT_20_SELF_END=5.00 PRIMER_RIGHT_20_SELF_END=1.00 PRIMER_LEFT_20_END_STABILITY=9.7000 PRIMER_RIGHT_20_END_STABILITY=7.9000 PRIMER_PAIR_20_COMPL_ANY=4.00 PRIMER_PAIR_20_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_20=150 PRIMER_PAIR_PENALTY_21=1.3699 PRIMER_LEFT_21_PENALTY=0.683672 PRIMER_RIGHT_21_PENALTY=0.686256 PRIMER_LEFT_21_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_21_SEQUENCE=CACGCATGTAGAGGTTCAGG PRIMER_LEFT_21=88,20 PRIMER_RIGHT_21=234,20 PRIMER_LEFT_21_TM=59.316 PRIMER_RIGHT_21_TM=59.314 PRIMER_LEFT_21_GC_PERCENT=45.000 PRIMER_RIGHT_21_GC_PERCENT=55.000 PRIMER_LEFT_21_SELF_ANY=5.00 PRIMER_RIGHT_21_SELF_ANY=4.00 PRIMER_LEFT_21_SELF_END=5.00 PRIMER_RIGHT_21_SELF_END=0.00 PRIMER_LEFT_21_END_STABILITY=9.7000 PRIMER_RIGHT_21_END_STABILITY=8.2000 PRIMER_PAIR_21_COMPL_ANY=4.00 PRIMER_PAIR_21_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_21=147 PRIMER_PAIR_PENALTY_22=1.3699 PRIMER_LEFT_22_PENALTY=0.683672 PRIMER_RIGHT_22_PENALTY=0.686256 PRIMER_LEFT_22_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_22_SEQUENCE=CGCATGTAGAGGTTCAGGTG PRIMER_LEFT_22=88,20 PRIMER_RIGHT_22=232,20 PRIMER_LEFT_22_TM=59.316 PRIMER_RIGHT_22_TM=59.314 PRIMER_LEFT_22_GC_PERCENT=45.000 PRIMER_RIGHT_22_GC_PERCENT=55.000 PRIMER_LEFT_22_SELF_ANY=5.00 PRIMER_RIGHT_22_SELF_ANY=4.00 PRIMER_LEFT_22_SELF_END=5.00 PRIMER_RIGHT_22_SELF_END=2.00 PRIMER_LEFT_22_END_STABILITY=9.7000 PRIMER_RIGHT_22_END_STABILITY=7.9000 PRIMER_PAIR_22_COMPL_ANY=4.00 PRIMER_PAIR_22_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_22=145 PRIMER_PAIR_PENALTY_23=1.4139 PRIMER_LEFT_23_PENALTY=0.775718 PRIMER_RIGHT_23_PENALTY=0.638191 PRIMER_LEFT_23_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_23_SEQUENCE=AGAGGTTCAGGTGGAGAGGG PRIMER_LEFT_23=87,20 PRIMER_RIGHT_23=225,20 PRIMER_LEFT_23_TM=60.776 PRIMER_RIGHT_23_TM=60.638 PRIMER_LEFT_23_GC_PERCENT=45.000 PRIMER_RIGHT_23_GC_PERCENT=60.000 PRIMER_LEFT_23_SELF_ANY=4.00 PRIMER_RIGHT_23_SELF_ANY=2.00 PRIMER_LEFT_23_SELF_END=3.00 PRIMER_RIGHT_23_SELF_END=0.00 PRIMER_LEFT_23_END_STABILITY=9.4000 PRIMER_RIGHT_23_END_STABILITY=9.4000 PRIMER_PAIR_23_COMPL_ANY=3.00 PRIMER_PAIR_23_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_23=139 PRIMER_PAIR_PENALTY_24=1.4139 PRIMER_LEFT_24_PENALTY=0.775718 PRIMER_RIGHT_24_PENALTY=0.638191 PRIMER_LEFT_24_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_24_SEQUENCE=AGAGGTTCAGGTGGAGAGGG PRIMER_LEFT_24=86,20 PRIMER_RIGHT_24=225,20 PRIMER_LEFT_24_TM=60.776 PRIMER_RIGHT_24_TM=60.638 PRIMER_LEFT_24_GC_PERCENT=50.000 PRIMER_RIGHT_24_GC_PERCENT=60.000 PRIMER_LEFT_24_SELF_ANY=5.00 PRIMER_RIGHT_24_SELF_ANY=2.00 PRIMER_LEFT_24_SELF_END=2.00 PRIMER_RIGHT_24_SELF_END=0.00 PRIMER_LEFT_24_END_STABILITY=9.7000 PRIMER_RIGHT_24_END_STABILITY=9.4000 PRIMER_PAIR_24_COMPL_ANY=4.00 PRIMER_PAIR_24_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_24=140 PRIMER_PAIR_PENALTY_25=1.4178 PRIMER_LEFT_25_PENALTY=0.151542 PRIMER_RIGHT_25_PENALTY=1.266285 PRIMER_LEFT_25_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_25_SEQUENCE=AACATGTGCACGCATGTAGA PRIMER_LEFT_25=76,20 PRIMER_RIGHT_25=242,20 PRIMER_LEFT_25_TM=60.152 PRIMER_RIGHT_25_TM=58.734 PRIMER_LEFT_25_GC_PERCENT=50.000 PRIMER_RIGHT_25_GC_PERCENT=45.000 PRIMER_LEFT_25_SELF_ANY=5.00 PRIMER_RIGHT_25_SELF_ANY=8.00 PRIMER_LEFT_25_SELF_END=3.00 PRIMER_RIGHT_25_SELF_END=4.00 PRIMER_LEFT_25_END_STABILITY=9.7000 PRIMER_RIGHT_25_END_STABILITY=5.4000 PRIMER_PAIR_25_COMPL_ANY=4.00 PRIMER_PAIR_25_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_25=167 PRIMER_PAIR_PENALTY_26=1.4178 PRIMER_LEFT_26_PENALTY=0.151542 PRIMER_RIGHT_26_PENALTY=1.266285 PRIMER_LEFT_26_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_26_SEQUENCE=GAACATGTGCACGCATGTAG PRIMER_LEFT_26=76,20 PRIMER_RIGHT_26=243,20 PRIMER_LEFT_26_TM=60.152 PRIMER_RIGHT_26_TM=58.734 PRIMER_LEFT_26_GC_PERCENT=50.000 PRIMER_RIGHT_26_GC_PERCENT=50.000 PRIMER_LEFT_26_SELF_ANY=5.00 PRIMER_RIGHT_26_SELF_ANY=8.00 PRIMER_LEFT_26_SELF_END=3.00 PRIMER_RIGHT_26_SELF_END=4.00 PRIMER_LEFT_26_END_STABILITY=9.7000 PRIMER_RIGHT_26_END_STABILITY=5.7000 PRIMER_PAIR_26_COMPL_ANY=4.00 PRIMER_PAIR_26_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_26=168 PRIMER_PAIR_PENALTY_27=1.4502 PRIMER_LEFT_27_PENALTY=0.683672 PRIMER_RIGHT_27_PENALTY=0.766499 PRIMER_LEFT_27_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_27_SEQUENCE=TGAACATGTGCACGCATGTA PRIMER_LEFT_27=88,20 PRIMER_RIGHT_27=244,20 PRIMER_LEFT_27_TM=59.316 PRIMER_RIGHT_27_TM=60.766 PRIMER_LEFT_27_GC_PERCENT=45.000 PRIMER_RIGHT_27_GC_PERCENT=45.000 PRIMER_LEFT_27_SELF_ANY=5.00 PRIMER_RIGHT_27_SELF_ANY=8.00 PRIMER_LEFT_27_SELF_END=5.00 PRIMER_RIGHT_27_SELF_END=6.00 PRIMER_LEFT_27_END_STABILITY=9.7000 PRIMER_RIGHT_27_END_STABILITY=5.6000 PRIMER_PAIR_27_COMPL_ANY=5.00 PRIMER_PAIR_27_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_27=157 PRIMER_PAIR_PENALTY_28=1.4527 PRIMER_LEFT_28_PENALTY=0.775718 PRIMER_RIGHT_28_PENALTY=0.676947 PRIMER_LEFT_28_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_28_SEQUENCE=GTGCACGCATGTAGAGGTTC PRIMER_LEFT_28=86,20 PRIMER_RIGHT_28=237,20 PRIMER_LEFT_28_TM=60.776 PRIMER_RIGHT_28_TM=59.323 PRIMER_LEFT_28_GC_PERCENT=50.000 PRIMER_RIGHT_28_GC_PERCENT=55.000 PRIMER_LEFT_28_SELF_ANY=5.00 PRIMER_RIGHT_28_SELF_ANY=6.00 PRIMER_LEFT_28_SELF_END=2.00 PRIMER_RIGHT_28_SELF_END=1.00 PRIMER_LEFT_28_END_STABILITY=9.7000 PRIMER_RIGHT_28_END_STABILITY=7.9000 PRIMER_PAIR_28_COMPL_ANY=4.00 PRIMER_PAIR_28_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_28=152 PRIMER_PAIR_PENALTY_29=1.4527 PRIMER_LEFT_29_PENALTY=0.775718 PRIMER_RIGHT_29_PENALTY=0.676947 PRIMER_LEFT_29_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_29_SEQUENCE=GTGCACGCATGTAGAGGTTC PRIMER_LEFT_29=87,20 PRIMER_RIGHT_29=237,20 PRIMER_LEFT_29_TM=60.776 PRIMER_RIGHT_29_TM=59.323 PRIMER_LEFT_29_GC_PERCENT=45.000 PRIMER_RIGHT_29_GC_PERCENT=55.000 PRIMER_LEFT_29_SELF_ANY=4.00 PRIMER_RIGHT_29_SELF_ANY=6.00 PRIMER_LEFT_29_SELF_END=3.00 PRIMER_RIGHT_29_SELF_END=1.00 PRIMER_LEFT_29_END_STABILITY=9.4000 PRIMER_RIGHT_29_END_STABILITY=7.9000 PRIMER_PAIR_29_COMPL_ANY=4.00 PRIMER_PAIR_29_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_29=151 PRIMER_PAIR_PENALTY_30=1.4620 PRIMER_LEFT_30_PENALTY=0.775718 PRIMER_RIGHT_30_PENALTY=0.686256 PRIMER_LEFT_30_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_30_SEQUENCE=CACGCATGTAGAGGTTCAGG PRIMER_LEFT_30=86,20 PRIMER_RIGHT_30=234,20 PRIMER_LEFT_30_TM=60.776 PRIMER_RIGHT_30_TM=59.314 PRIMER_LEFT_30_GC_PERCENT=50.000 PRIMER_RIGHT_30_GC_PERCENT=55.000 PRIMER_LEFT_30_SELF_ANY=5.00 PRIMER_RIGHT_30_SELF_ANY=4.00 PRIMER_LEFT_30_SELF_END=2.00 PRIMER_RIGHT_30_SELF_END=0.00 PRIMER_LEFT_30_END_STABILITY=9.7000 PRIMER_RIGHT_30_END_STABILITY=8.2000 PRIMER_PAIR_30_COMPL_ANY=4.00 PRIMER_PAIR_30_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_30=149 PRIMER_PAIR_PENALTY_31=1.4620 PRIMER_LEFT_31_PENALTY=0.775718 PRIMER_RIGHT_31_PENALTY=0.686256 PRIMER_LEFT_31_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_31_SEQUENCE=CACGCATGTAGAGGTTCAGG PRIMER_LEFT_31=87,20 PRIMER_RIGHT_31=234,20 PRIMER_LEFT_31_TM=60.776 PRIMER_RIGHT_31_TM=59.314 PRIMER_LEFT_31_GC_PERCENT=45.000 PRIMER_RIGHT_31_GC_PERCENT=55.000 PRIMER_LEFT_31_SELF_ANY=4.00 PRIMER_RIGHT_31_SELF_ANY=4.00 PRIMER_LEFT_31_SELF_END=3.00 PRIMER_RIGHT_31_SELF_END=0.00 PRIMER_LEFT_31_END_STABILITY=9.4000 PRIMER_RIGHT_31_END_STABILITY=8.2000 PRIMER_PAIR_31_COMPL_ANY=4.00 PRIMER_PAIR_31_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_31=148 PRIMER_PAIR_PENALTY_32=1.4620 PRIMER_LEFT_32_PENALTY=0.775718 PRIMER_RIGHT_32_PENALTY=0.686256 PRIMER_LEFT_32_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_32_SEQUENCE=CGCATGTAGAGGTTCAGGTG PRIMER_LEFT_32=87,20 PRIMER_RIGHT_32=232,20 PRIMER_LEFT_32_TM=60.776 PRIMER_RIGHT_32_TM=59.314 PRIMER_LEFT_32_GC_PERCENT=45.000 PRIMER_RIGHT_32_GC_PERCENT=55.000 PRIMER_LEFT_32_SELF_ANY=4.00 PRIMER_RIGHT_32_SELF_ANY=4.00 PRIMER_LEFT_32_SELF_END=3.00 PRIMER_RIGHT_32_SELF_END=2.00 PRIMER_LEFT_32_END_STABILITY=9.4000 PRIMER_RIGHT_32_END_STABILITY=7.9000 PRIMER_PAIR_32_COMPL_ANY=4.00 PRIMER_PAIR_32_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_32=146 PRIMER_PAIR_PENALTY_33=1.4620 PRIMER_LEFT_33_PENALTY=0.775718 PRIMER_RIGHT_33_PENALTY=0.686256 PRIMER_LEFT_33_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_33_SEQUENCE=CGCATGTAGAGGTTCAGGTG PRIMER_LEFT_33=86,20 PRIMER_RIGHT_33=232,20 PRIMER_LEFT_33_TM=60.776 PRIMER_RIGHT_33_TM=59.314 PRIMER_LEFT_33_GC_PERCENT=50.000 PRIMER_RIGHT_33_GC_PERCENT=55.000 PRIMER_LEFT_33_SELF_ANY=5.00 PRIMER_RIGHT_33_SELF_ANY=4.00 PRIMER_LEFT_33_SELF_END=2.00 PRIMER_RIGHT_33_SELF_END=2.00 PRIMER_LEFT_33_END_STABILITY=9.7000 PRIMER_RIGHT_33_END_STABILITY=7.9000 PRIMER_PAIR_33_COMPL_ANY=4.00 PRIMER_PAIR_33_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_33=147 PRIMER_PAIR_PENALTY_34=1.4697 PRIMER_LEFT_34_PENALTY=0.151542 PRIMER_RIGHT_34_PENALTY=1.318201 PRIMER_LEFT_34_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_34_SEQUENCE=CATGTGCACGCATGTAGAGG PRIMER_LEFT_34=76,20 PRIMER_RIGHT_34=240,20 PRIMER_LEFT_34_TM=60.152 PRIMER_RIGHT_34_TM=61.318 PRIMER_LEFT_34_GC_PERCENT=50.000 PRIMER_RIGHT_34_GC_PERCENT=55.000 PRIMER_LEFT_34_SELF_ANY=5.00 PRIMER_RIGHT_34_SELF_ANY=6.00 PRIMER_LEFT_34_SELF_END=3.00 PRIMER_RIGHT_34_SELF_END=4.00 PRIMER_LEFT_34_END_STABILITY=9.7000 PRIMER_RIGHT_34_END_STABILITY=7.9000 PRIMER_PAIR_34_COMPL_ANY=4.00 PRIMER_PAIR_34_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_34=165 PRIMER_PAIR_PENALTY_35=1.4884 PRIMER_LEFT_35_PENALTY=0.683672 PRIMER_RIGHT_35_PENALTY=0.804687 PRIMER_LEFT_35_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_35_SEQUENCE=ATGTGCACGCATGTAGAGGT PRIMER_LEFT_35=88,20 PRIMER_RIGHT_35=239,20 PRIMER_LEFT_35_TM=59.316 PRIMER_RIGHT_35_TM=59.195 PRIMER_LEFT_35_GC_PERCENT=45.000 PRIMER_RIGHT_35_GC_PERCENT=50.000 PRIMER_LEFT_35_SELF_ANY=5.00 PRIMER_RIGHT_35_SELF_ANY=6.00 PRIMER_LEFT_35_SELF_END=5.00 PRIMER_RIGHT_35_SELF_END=0.00 PRIMER_LEFT_35_END_STABILITY=9.7000 PRIMER_RIGHT_35_END_STABILITY=7.6000 PRIMER_PAIR_35_COMPL_ANY=4.00 PRIMER_PAIR_35_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_35=152 PRIMER_PAIR_PENALTY_36=1.5294 PRIMER_LEFT_36_PENALTY=0.151542 PRIMER_RIGHT_36_PENALTY=1.377815 PRIMER_LEFT_36_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_36_SEQUENCE=GAGGTTCAGGTGGAGAGGG PRIMER_LEFT_36=76,20 PRIMER_RIGHT_36=224,19 PRIMER_LEFT_36_TM=60.152 PRIMER_RIGHT_36_TM=59.622 PRIMER_LEFT_36_GC_PERCENT=50.000 PRIMER_RIGHT_36_GC_PERCENT=63.158 PRIMER_LEFT_36_SELF_ANY=5.00 PRIMER_RIGHT_36_SELF_ANY=2.00 PRIMER_LEFT_36_SELF_END=3.00 PRIMER_RIGHT_36_SELF_END=0.00 PRIMER_LEFT_36_END_STABILITY=9.7000 PRIMER_RIGHT_36_END_STABILITY=9.4000 PRIMER_PAIR_36_COMPL_ANY=4.00 PRIMER_PAIR_36_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_36=149 PRIMER_PAIR_PENALTY_37=1.5422 PRIMER_LEFT_37_PENALTY=0.775718 PRIMER_RIGHT_37_PENALTY=0.766499 PRIMER_LEFT_37_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_37_SEQUENCE=TGAACATGTGCACGCATGTA PRIMER_LEFT_37=86,20 PRIMER_RIGHT_37=244,20 PRIMER_LEFT_37_TM=60.776 PRIMER_RIGHT_37_TM=60.766 PRIMER_LEFT_37_GC_PERCENT=50.000 PRIMER_RIGHT_37_GC_PERCENT=45.000 PRIMER_LEFT_37_SELF_ANY=5.00 PRIMER_RIGHT_37_SELF_ANY=8.00 PRIMER_LEFT_37_SELF_END=2.00 PRIMER_RIGHT_37_SELF_END=6.00 PRIMER_LEFT_37_END_STABILITY=9.7000 PRIMER_RIGHT_37_END_STABILITY=5.6000 PRIMER_PAIR_37_COMPL_ANY=5.00 PRIMER_PAIR_37_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_37=159 PRIMER_PAIR_PENALTY_38=1.5422 PRIMER_LEFT_38_PENALTY=0.775718 PRIMER_RIGHT_38_PENALTY=0.766499 PRIMER_LEFT_38_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_38_SEQUENCE=TGAACATGTGCACGCATGTA PRIMER_LEFT_38=87,20 PRIMER_RIGHT_38=244,20 PRIMER_LEFT_38_TM=60.776 PRIMER_RIGHT_38_TM=60.766 PRIMER_LEFT_38_GC_PERCENT=45.000 PRIMER_RIGHT_38_GC_PERCENT=45.000 PRIMER_LEFT_38_SELF_ANY=4.00 PRIMER_RIGHT_38_SELF_ANY=8.00 PRIMER_LEFT_38_SELF_END=3.00 PRIMER_RIGHT_38_SELF_END=6.00 PRIMER_LEFT_38_END_STABILITY=9.4000 PRIMER_RIGHT_38_END_STABILITY=5.6000 PRIMER_PAIR_38_COMPL_ANY=5.00 PRIMER_PAIR_38_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_38=158 PRIMER_PAIR_PENALTY_39=1.5431 PRIMER_LEFT_39_PENALTY=1.210088 PRIMER_RIGHT_39_PENALTY=0.333053 PRIMER_LEFT_39_SEQUENCE=TGACAGGCAATTTCAAGGC PRIMER_RIGHT_39_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_39=87,19 PRIMER_RIGHT_39=238,20 PRIMER_LEFT_39_TM=59.790 PRIMER_RIGHT_39_TM=60.333 PRIMER_LEFT_39_GC_PERCENT=47.368 PRIMER_RIGHT_39_GC_PERCENT=50.000 PRIMER_LEFT_39_SELF_ANY=4.00 PRIMER_RIGHT_39_SELF_ANY=6.00 PRIMER_LEFT_39_SELF_END=2.00 PRIMER_RIGHT_39_SELF_END=0.00 PRIMER_LEFT_39_END_STABILITY=9.7000 PRIMER_RIGHT_39_END_STABILITY=7.9000 PRIMER_PAIR_39_COMPL_ANY=4.00 PRIMER_PAIR_39_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_39=152 = MARKER_NAME=08.RHAP34FE8.seq SEQUENCE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG INCLUDED_REGION=20,281 TARGET=119,62,AC PRIMER_PAIR_PENALTY=0.2190 PRIMER_LEFT_PENALTY=0.201280 PRIMER_RIGHT_PENALTY=0.017711 PRIMER_LEFT_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT=53,20 PRIMER_RIGHT=220,20 PRIMER_LEFT_TM=59.799 PRIMER_RIGHT_TM=59.982 PRIMER_LEFT_GC_PERCENT=50.000 PRIMER_RIGHT_GC_PERCENT=55.000 PRIMER_LEFT_SELF_ANY=7.00 PRIMER_RIGHT_SELF_ANY=6.00 PRIMER_LEFT_SELF_END=5.00 PRIMER_RIGHT_SELF_END=4.00 PRIMER_LEFT_END_STABILITY=7.9000 PRIMER_RIGHT_END_STABILITY=9.7000 PRIMER_PAIR_MAX_COMPL_ANY=4.00 PRIMER_PAIR_MAX_COMPL_END=4.00 PRIMER_PRODUCT_SIZE=168 PRIMER_PAIR_PENALTY_1=0.2677 PRIMER_LEFT_1_PENALTY=0.249989 PRIMER_RIGHT_1_PENALTY=0.017711 PRIMER_LEFT_1_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_1_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_1=83,20 PRIMER_RIGHT_1=220,20 PRIMER_LEFT_1_TM=59.750 PRIMER_RIGHT_1_TM=59.982 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_1=138 PRIMER_PAIR_PENALTY_2=0.3573 PRIMER_LEFT_2_PENALTY=0.201280 PRIMER_RIGHT_2_PENALTY=0.156061 PRIMER_LEFT_2_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_2_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_2=53,20 PRIMER_RIGHT_2=218,20 PRIMER_LEFT_2_TM=59.799 PRIMER_RIGHT_2_TM=59.844 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_2=166 PRIMER_PAIR_PENALTY_3=0.4060 PRIMER_LEFT_3_PENALTY=0.249989 PRIMER_RIGHT_3_PENALTY=0.156061 PRIMER_LEFT_3_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_3_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_3=83,20 PRIMER_RIGHT_3=218,20 PRIMER_LEFT_3_TM=59.750 PRIMER_RIGHT_3_TM=59.844 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_3=136 PRIMER_PAIR_PENALTY_4=0.4406 PRIMER_LEFT_4_PENALTY=0.422888 PRIMER_RIGHT_4_PENALTY=0.017711 PRIMER_LEFT_4_SEQUENCE=GCTTTTTCCCCTTAGGACCA PRIMER_RIGHT_4_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_4=49,20 PRIMER_RIGHT_4=220,20 PRIMER_LEFT_4_TM=60.423 PRIMER_RIGHT_4_TM=59.982 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_4=172 PRIMER_PAIR_PENALTY_5=0.5102 PRIMER_LEFT_5_PENALTY=0.201280 PRIMER_RIGHT_5_PENALTY=0.308871 PRIMER_LEFT_5_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_5_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_5=53,20 PRIMER_RIGHT_5=217,20 PRIMER_LEFT_5_TM=59.799 PRIMER_RIGHT_5_TM=59.691 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=55.000 PRIMER_LEFT_5_SELF_ANY=7.00 PRIMER_RIGHT_5_SELF_ANY=5.00 PRIMER_LEFT_5_SELF_END=5.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_END_STABILITY=7.9000 PRIMER_RIGHT_5_END_STABILITY=9.4000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_5=165 PRIMER_PAIR_PENALTY_6=0.5433 PRIMER_LEFT_6_PENALTY=0.525579 PRIMER_RIGHT_6_PENALTY=0.017711 PRIMER_LEFT_6_SEQUENCE=GGACCACGGATTATCCTTCC PRIMER_RIGHT_6_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_6=82,20 PRIMER_RIGHT_6=220,20 PRIMER_LEFT_6_TM=60.526 PRIMER_RIGHT_6_TM=59.982 PRIMER_LEFT_6_GC_PERCENT=55.000 PRIMER_RIGHT_6_GC_PERCENT=55.000 PRIMER_LEFT_6_SELF_ANY=7.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=4.00 PRIMER_LEFT_6_END_STABILITY=8.2000 PRIMER_RIGHT_6_END_STABILITY=9.7000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_6=139 PRIMER_PAIR_PENALTY_7=0.5589 PRIMER_LEFT_7_PENALTY=0.249989 PRIMER_RIGHT_7_PENALTY=0.308871 PRIMER_LEFT_7_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_7_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_7=83,20 PRIMER_RIGHT_7=217,20 PRIMER_LEFT_7_TM=59.750 PRIMER_RIGHT_7_TM=59.691 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=55.000 PRIMER_LEFT_7_SELF_ANY=7.00 PRIMER_RIGHT_7_SELF_ANY=5.00 PRIMER_LEFT_7_SELF_END=1.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_END_STABILITY=8.5000 PRIMER_RIGHT_7_END_STABILITY=9.4000 PRIMER_PAIR_7_COMPL_ANY=5.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_7=135 PRIMER_PAIR_PENALTY_8=0.5789 PRIMER_LEFT_8_PENALTY=0.422888 PRIMER_RIGHT_8_PENALTY=0.156061 PRIMER_LEFT_8_SEQUENCE=GCTTTTTCCCCTTAGGACCA PRIMER_RIGHT_8_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_8=49,20 PRIMER_RIGHT_8=218,20 PRIMER_LEFT_8_TM=60.423 PRIMER_RIGHT_8_TM=59.844 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=55.000 PRIMER_LEFT_8_SELF_ANY=5.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_LEFT_8_SELF_END=1.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_END_STABILITY=7.9000 PRIMER_RIGHT_8_END_STABILITY=8.2000 PRIMER_PAIR_8_COMPL_ANY=4.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_8=170 PRIMER_PAIR_PENALTY_9=0.6816 PRIMER_LEFT_9_PENALTY=0.525579 PRIMER_RIGHT_9_PENALTY=0.156061 PRIMER_LEFT_9_SEQUENCE=GGACCACGGATTATCCTTCC PRIMER_RIGHT_9_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_9=82,20 PRIMER_RIGHT_9=218,20 PRIMER_LEFT_9_TM=60.526 PRIMER_RIGHT_9_TM=59.844 PRIMER_LEFT_9_GC_PERCENT=55.000 PRIMER_RIGHT_9_GC_PERCENT=55.000 PRIMER_LEFT_9_SELF_ANY=7.00 PRIMER_RIGHT_9_SELF_ANY=5.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_END_STABILITY=8.2000 PRIMER_RIGHT_9_END_STABILITY=8.2000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_9=137 PRIMER_PAIR_PENALTY_10=0.7318 PRIMER_LEFT_10_PENALTY=0.422888 PRIMER_RIGHT_10_PENALTY=0.308871 PRIMER_LEFT_10_SEQUENCE=GCTTTTTCCCCTTAGGACCA PRIMER_RIGHT_10_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_10=49,20 PRIMER_RIGHT_10=217,20 PRIMER_LEFT_10_TM=60.423 PRIMER_RIGHT_10_TM=59.691 PRIMER_LEFT_10_GC_PERCENT=50.000 PRIMER_RIGHT_10_GC_PERCENT=55.000 PRIMER_LEFT_10_SELF_ANY=5.00 PRIMER_RIGHT_10_SELF_ANY=5.00 PRIMER_LEFT_10_SELF_END=1.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_END_STABILITY=7.9000 PRIMER_RIGHT_10_END_STABILITY=9.4000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_10=169 PRIMER_PAIR_PENALTY_11=0.8345 PRIMER_LEFT_11_PENALTY=0.525579 PRIMER_RIGHT_11_PENALTY=0.308871 PRIMER_LEFT_11_SEQUENCE=GGACCACGGATTATCCTTCC PRIMER_RIGHT_11_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_11=82,20 PRIMER_RIGHT_11=217,20 PRIMER_LEFT_11_TM=60.526 PRIMER_RIGHT_11_TM=59.691 PRIMER_LEFT_11_GC_PERCENT=55.000 PRIMER_RIGHT_11_GC_PERCENT=55.000 PRIMER_LEFT_11_SELF_ANY=7.00 PRIMER_RIGHT_11_SELF_ANY=5.00 PRIMER_LEFT_11_SELF_END=3.00 PRIMER_RIGHT_11_SELF_END=1.00 PRIMER_LEFT_11_END_STABILITY=8.2000 PRIMER_RIGHT_11_END_STABILITY=9.4000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_11=136 PRIMER_PAIR_PENALTY_12=0.9709 PRIMER_LEFT_12_PENALTY=0.201280 PRIMER_RIGHT_12_PENALTY=0.769636 PRIMER_LEFT_12_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_12_SEQUENCE=AAGGACCTGGTGGGACAGAT PRIMER_LEFT_12=53,20 PRIMER_RIGHT_12=210,20 PRIMER_LEFT_12_TM=59.799 PRIMER_RIGHT_12_TM=60.770 PRIMER_LEFT_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_GC_PERCENT=55.000 PRIMER_LEFT_12_SELF_ANY=7.00 PRIMER_RIGHT_12_SELF_ANY=5.00 PRIMER_LEFT_12_SELF_END=5.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_END_STABILITY=7.9000 PRIMER_RIGHT_12_END_STABILITY=6.6000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_12=158 PRIMER_PAIR_PENALTY_13=1.0196 PRIMER_LEFT_13_PENALTY=0.249989 PRIMER_RIGHT_13_PENALTY=0.769636 PRIMER_LEFT_13_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_13_SEQUENCE=AAGGACCTGGTGGGACAGAT PRIMER_LEFT_13=83,20 PRIMER_RIGHT_13=210,20 PRIMER_LEFT_13_TM=59.750 PRIMER_RIGHT_13_TM=60.770 PRIMER_LEFT_13_GC_PERCENT=50.000 PRIMER_RIGHT_13_GC_PERCENT=55.000 PRIMER_LEFT_13_SELF_ANY=7.00 PRIMER_RIGHT_13_SELF_ANY=5.00 PRIMER_LEFT_13_SELF_END=1.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_END_STABILITY=8.5000 PRIMER_RIGHT_13_END_STABILITY=6.6000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_13=128 PRIMER_PAIR_PENALTY_14=1.0446 PRIMER_LEFT_14_PENALTY=1.026872 PRIMER_RIGHT_14_PENALTY=0.017711 PRIMER_LEFT_14_SEQUENCE=CCACGGATTATCCTTCCATTT PRIMER_RIGHT_14_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_14=85,21 PRIMER_RIGHT_14=220,20 PRIMER_LEFT_14_TM=60.027 PRIMER_RIGHT_14_TM=59.982 PRIMER_LEFT_14_GC_PERCENT=42.857 PRIMER_RIGHT_14_GC_PERCENT=55.000 PRIMER_LEFT_14_SELF_ANY=7.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=4.00 PRIMER_LEFT_14_END_STABILITY=7.2000 PRIMER_RIGHT_14_END_STABILITY=9.7000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_14=136 PRIMER_PAIR_PENALTY_15=1.0492 PRIMER_LEFT_15_PENALTY=1.031536 PRIMER_RIGHT_15_PENALTY=0.017711 PRIMER_LEFT_15_SEQUENCE=GACCACGGATTATCCTTCCAT PRIMER_RIGHT_15_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_15=83,21 PRIMER_RIGHT_15=220,20 PRIMER_LEFT_15_TM=60.032 PRIMER_RIGHT_15_TM=59.982 PRIMER_LEFT_15_GC_PERCENT=47.619 PRIMER_RIGHT_15_GC_PERCENT=55.000 PRIMER_LEFT_15_SELF_ANY=7.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=4.00 PRIMER_LEFT_15_END_STABILITY=8.1000 PRIMER_RIGHT_15_END_STABILITY=9.7000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_15=138 PRIMER_PAIR_PENALTY_16=1.1169 PRIMER_LEFT_16_PENALTY=1.099227 PRIMER_RIGHT_16_PENALTY=0.017711 PRIMER_LEFT_16_SEQUENCE=TCAGCTTTTTCCCCTTAGGA PRIMER_RIGHT_16_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_16=46,20 PRIMER_RIGHT_16=220,20 PRIMER_LEFT_16_TM=58.901 PRIMER_RIGHT_16_TM=59.982 PRIMER_LEFT_16_GC_PERCENT=45.000 PRIMER_RIGHT_16_GC_PERCENT=55.000 PRIMER_LEFT_16_SELF_ANY=5.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=4.00 PRIMER_LEFT_16_END_STABILITY=7.2000 PRIMER_RIGHT_16_END_STABILITY=9.7000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_16=175 PRIMER_PAIR_PENALTY_17=1.1538 PRIMER_LEFT_17_PENALTY=1.136051 PRIMER_RIGHT_17_PENALTY=0.017711 PRIMER_LEFT_17_SEQUENCE=GGTATAGCCTTAGGACCACGG PRIMER_RIGHT_17_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_17=70,21 PRIMER_RIGHT_17=220,20 PRIMER_LEFT_17_TM=59.864 PRIMER_RIGHT_17_TM=59.982 PRIMER_LEFT_17_GC_PERCENT=57.143 PRIMER_RIGHT_17_GC_PERCENT=55.000 PRIMER_LEFT_17_SELF_ANY=5.00 PRIMER_RIGHT_17_SELF_ANY=6.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=4.00 PRIMER_LEFT_17_END_STABILITY=9.9000 PRIMER_RIGHT_17_END_STABILITY=9.7000 PRIMER_PAIR_17_COMPL_ANY=4.00 PRIMER_PAIR_17_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_17=151 PRIMER_PAIR_PENALTY_18=1.1829 PRIMER_LEFT_18_PENALTY=1.026872 PRIMER_RIGHT_18_PENALTY=0.156061 PRIMER_LEFT_18_SEQUENCE=CCACGGATTATCCTTCCATTT PRIMER_RIGHT_18_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_18=85,21 PRIMER_RIGHT_18=218,20 PRIMER_LEFT_18_TM=60.027 PRIMER_RIGHT_18_TM=59.844 PRIMER_LEFT_18_GC_PERCENT=42.857 PRIMER_RIGHT_18_GC_PERCENT=55.000 PRIMER_LEFT_18_SELF_ANY=7.00 PRIMER_RIGHT_18_SELF_ANY=5.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=3.00 PRIMER_LEFT_18_END_STABILITY=7.2000 PRIMER_RIGHT_18_END_STABILITY=8.2000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_18=134 PRIMER_PAIR_PENALTY_19=1.1876 PRIMER_LEFT_19_PENALTY=1.031536 PRIMER_RIGHT_19_PENALTY=0.156061 PRIMER_LEFT_19_SEQUENCE=GACCACGGATTATCCTTCCAT PRIMER_RIGHT_19_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_19=83,21 PRIMER_RIGHT_19=218,20 PRIMER_LEFT_19_TM=60.032 PRIMER_RIGHT_19_TM=59.844 PRIMER_LEFT_19_GC_PERCENT=47.619 PRIMER_RIGHT_19_GC_PERCENT=55.000 PRIMER_LEFT_19_SELF_ANY=7.00 PRIMER_RIGHT_19_SELF_ANY=5.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_END_STABILITY=8.1000 PRIMER_RIGHT_19_END_STABILITY=8.2000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_19=136 PRIMER_PAIR_PENALTY_20=1.1925 PRIMER_LEFT_20_PENALTY=0.422888 PRIMER_RIGHT_20_PENALTY=0.769636 PRIMER_LEFT_20_SEQUENCE=GCTTTTTCCCCTTAGGACCA PRIMER_RIGHT_20_SEQUENCE=AAGGACCTGGTGGGACAGAT PRIMER_LEFT_20=49,20 PRIMER_RIGHT_20=210,20 PRIMER_LEFT_20_TM=60.423 PRIMER_RIGHT_20_TM=60.770 PRIMER_LEFT_20_GC_PERCENT=50.000 PRIMER_RIGHT_20_GC_PERCENT=55.000 PRIMER_LEFT_20_SELF_ANY=5.00 PRIMER_RIGHT_20_SELF_ANY=5.00 PRIMER_LEFT_20_SELF_END=1.00 PRIMER_RIGHT_20_SELF_END=2.00 PRIMER_LEFT_20_END_STABILITY=7.9000 PRIMER_RIGHT_20_END_STABILITY=6.6000 PRIMER_PAIR_20_COMPL_ANY=5.00 PRIMER_PAIR_20_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_20=162 PRIMER_PAIR_PENALTY_21=1.2017 PRIMER_LEFT_21_PENALTY=1.184005 PRIMER_RIGHT_21_PENALTY=0.017711 PRIMER_LEFT_21_SEQUENCE=TTTCCCCTTAGGACCAAGGTA PRIMER_RIGHT_21_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_21=53,21 PRIMER_RIGHT_21=220,20 PRIMER_LEFT_21_TM=59.816 PRIMER_RIGHT_21_TM=59.982 PRIMER_LEFT_21_GC_PERCENT=47.619 PRIMER_RIGHT_21_GC_PERCENT=55.000 PRIMER_LEFT_21_SELF_ANY=7.00 PRIMER_RIGHT_21_SELF_ANY=6.00 PRIMER_LEFT_21_SELF_END=3.00 PRIMER_RIGHT_21_SELF_END=4.00 PRIMER_LEFT_21_END_STABILITY=6.9000 PRIMER_RIGHT_21_END_STABILITY=9.7000 PRIMER_PAIR_21_COMPL_ANY=4.00 PRIMER_PAIR_21_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_21=168 PRIMER_PAIR_PENALTY_22=1.2054 PRIMER_LEFT_22_PENALTY=1.187663 PRIMER_RIGHT_22_PENALTY=0.017711 PRIMER_LEFT_22_SEQUENCE=GCCTTAGGACCACGGATTATC PRIMER_RIGHT_22_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_22=76,21 PRIMER_RIGHT_22=220,20 PRIMER_LEFT_22_TM=59.812 PRIMER_RIGHT_22_TM=59.982 PRIMER_LEFT_22_GC_PERCENT=52.381 PRIMER_RIGHT_22_GC_PERCENT=55.000 PRIMER_LEFT_22_SELF_ANY=5.00 PRIMER_RIGHT_22_SELF_ANY=6.00 PRIMER_LEFT_22_SELF_END=5.00 PRIMER_RIGHT_22_SELF_END=4.00 PRIMER_LEFT_22_END_STABILITY=5.9000 PRIMER_RIGHT_22_END_STABILITY=9.7000 PRIMER_PAIR_22_COMPL_ANY=4.00 PRIMER_PAIR_22_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_22=145 PRIMER_PAIR_PENALTY_23=1.2553 PRIMER_LEFT_23_PENALTY=1.099227 PRIMER_RIGHT_23_PENALTY=0.156061 PRIMER_LEFT_23_SEQUENCE=TCAGCTTTTTCCCCTTAGGA PRIMER_RIGHT_23_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_23=46,20 PRIMER_RIGHT_23=218,20 PRIMER_LEFT_23_TM=58.901 PRIMER_RIGHT_23_TM=59.844 PRIMER_LEFT_23_GC_PERCENT=45.000 PRIMER_RIGHT_23_GC_PERCENT=55.000 PRIMER_LEFT_23_SELF_ANY=5.00 PRIMER_RIGHT_23_SELF_ANY=5.00 PRIMER_LEFT_23_SELF_END=3.00 PRIMER_RIGHT_23_SELF_END=3.00 PRIMER_LEFT_23_END_STABILITY=7.2000 PRIMER_RIGHT_23_END_STABILITY=8.2000 PRIMER_PAIR_23_COMPL_ANY=4.00 PRIMER_PAIR_23_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_23=173 PRIMER_PAIR_PENALTY_24=1.2554 PRIMER_LEFT_24_PENALTY=0.201280 PRIMER_RIGHT_24_PENALTY=1.054163 PRIMER_LEFT_24_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_24_SEQUENCE=AAAGGACCTGGTGGGACAG PRIMER_LEFT_24=53,20 PRIMER_RIGHT_24=211,19 PRIMER_LEFT_24_TM=59.799 PRIMER_RIGHT_24_TM=59.946 PRIMER_LEFT_24_GC_PERCENT=50.000 PRIMER_RIGHT_24_GC_PERCENT=57.895 PRIMER_LEFT_24_SELF_ANY=7.00 PRIMER_RIGHT_24_SELF_ANY=5.00 PRIMER_LEFT_24_SELF_END=5.00 PRIMER_RIGHT_24_SELF_END=1.00 PRIMER_LEFT_24_END_STABILITY=7.9000 PRIMER_RIGHT_24_END_STABILITY=6.4000 PRIMER_PAIR_24_COMPL_ANY=5.00 PRIMER_PAIR_24_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_24=159 PRIMER_PAIR_PENALTY_25=1.2921 PRIMER_LEFT_25_PENALTY=1.136051 PRIMER_RIGHT_25_PENALTY=0.156061 PRIMER_LEFT_25_SEQUENCE=GGTATAGCCTTAGGACCACGG PRIMER_RIGHT_25_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_25=70,21 PRIMER_RIGHT_25=218,20 PRIMER_LEFT_25_TM=59.864 PRIMER_RIGHT_25_TM=59.844 PRIMER_LEFT_25_GC_PERCENT=57.143 PRIMER_RIGHT_25_GC_PERCENT=55.000 PRIMER_LEFT_25_SELF_ANY=5.00 PRIMER_RIGHT_25_SELF_ANY=5.00 PRIMER_LEFT_25_SELF_END=3.00 PRIMER_RIGHT_25_SELF_END=3.00 PRIMER_LEFT_25_END_STABILITY=9.9000 PRIMER_RIGHT_25_END_STABILITY=8.2000 PRIMER_PAIR_25_COMPL_ANY=4.00 PRIMER_PAIR_25_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_25=149 PRIMER_PAIR_PENALTY_26=1.2952 PRIMER_LEFT_26_PENALTY=0.525579 PRIMER_RIGHT_26_PENALTY=0.769636 PRIMER_LEFT_26_SEQUENCE=GGACCACGGATTATCCTTCC PRIMER_RIGHT_26_SEQUENCE=AAGGACCTGGTGGGACAGAT PRIMER_LEFT_26=82,20 PRIMER_RIGHT_26=210,20 PRIMER_LEFT_26_TM=60.526 PRIMER_RIGHT_26_TM=60.770 PRIMER_LEFT_26_GC_PERCENT=55.000 PRIMER_RIGHT_26_GC_PERCENT=55.000 PRIMER_LEFT_26_SELF_ANY=7.00 PRIMER_RIGHT_26_SELF_ANY=5.00 PRIMER_LEFT_26_SELF_END=3.00 PRIMER_RIGHT_26_SELF_END=2.00 PRIMER_LEFT_26_END_STABILITY=8.2000 PRIMER_RIGHT_26_END_STABILITY=6.6000 PRIMER_PAIR_26_COMPL_ANY=5.00 PRIMER_PAIR_26_COMPL_END=4.00 PRIMER_PRODUCT_SIZE_26=129 PRIMER_PAIR_PENALTY_27=1.3030 PRIMER_LEFT_27_PENALTY=1.285273 PRIMER_RIGHT_27_PENALTY=0.017711 PRIMER_LEFT_27_SEQUENCE=CCACGGATTATCCTTCCATT PRIMER_RIGHT_27_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_27=85,20 PRIMER_RIGHT_27=220,20 PRIMER_LEFT_27_TM=58.715 PRIMER_RIGHT_27_TM=59.982 PRIMER_LEFT_27_GC_PERCENT=45.000 PRIMER_RIGHT_27_GC_PERCENT=55.000 PRIMER_LEFT_27_SELF_ANY=7.00 PRIMER_RIGHT_27_SELF_ANY=6.00 PRIMER_LEFT_27_SELF_END=1.00 PRIMER_RIGHT_27_SELF_END=4.00 PRIMER_LEFT_27_END_STABILITY=8.4000 PRIMER_RIGHT_27_END_STABILITY=9.7000 PRIMER_PAIR_27_COMPL_ANY=5.00 PRIMER_PAIR_27_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_27=136 PRIMER_PAIR_PENALTY_28=1.3042 PRIMER_LEFT_28_PENALTY=0.249989 PRIMER_RIGHT_28_PENALTY=1.054163 PRIMER_LEFT_28_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_28_SEQUENCE=AAAGGACCTGGTGGGACAG PRIMER_LEFT_28=83,20 PRIMER_RIGHT_28=211,19 PRIMER_LEFT_28_TM=59.750 PRIMER_RIGHT_28_TM=59.946 PRIMER_LEFT_28_GC_PERCENT=50.000 PRIMER_RIGHT_28_GC_PERCENT=57.895 PRIMER_LEFT_28_SELF_ANY=7.00 PRIMER_RIGHT_28_SELF_ANY=5.00 PRIMER_LEFT_28_SELF_END=1.00 PRIMER_RIGHT_28_SELF_END=1.00 PRIMER_LEFT_28_END_STABILITY=8.5000 PRIMER_RIGHT_28_END_STABILITY=6.4000 PRIMER_PAIR_28_COMPL_ANY=5.00 PRIMER_PAIR_28_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_28=129 PRIMER_PAIR_PENALTY_29=1.3055 PRIMER_LEFT_29_PENALTY=1.287819 PRIMER_RIGHT_29_PENALTY=0.017711 PRIMER_LEFT_29_SEQUENCE=AGCTTTTTCCCCTTAGGACC PRIMER_RIGHT_29_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_29=48,20 PRIMER_RIGHT_29=220,20 PRIMER_LEFT_29_TM=58.712 PRIMER_RIGHT_29_TM=59.982 PRIMER_LEFT_29_GC_PERCENT=50.000 PRIMER_RIGHT_29_GC_PERCENT=55.000 PRIMER_LEFT_29_SELF_ANY=5.00 PRIMER_RIGHT_29_SELF_ANY=6.00 PRIMER_LEFT_29_SELF_END=3.00 PRIMER_RIGHT_29_SELF_END=4.00 PRIMER_LEFT_29_END_STABILITY=9.1000 PRIMER_RIGHT_29_END_STABILITY=9.7000 PRIMER_PAIR_29_COMPL_ANY=4.00 PRIMER_PAIR_29_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_29=173 PRIMER_PAIR_PENALTY_30=1.3059 PRIMER_LEFT_30_PENALTY=1.288173 PRIMER_RIGHT_30_PENALTY=0.017711 PRIMER_LEFT_30_SEQUENCE=TCAGCTTTTTCCCCTTAGGAC PRIMER_RIGHT_30_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_30=46,21 PRIMER_RIGHT_30=220,20 PRIMER_LEFT_30_TM=59.712 PRIMER_RIGHT_30_TM=59.982 PRIMER_LEFT_30_GC_PERCENT=47.619 PRIMER_RIGHT_30_GC_PERCENT=55.000 PRIMER_LEFT_30_SELF_ANY=5.00 PRIMER_RIGHT_30_SELF_ANY=6.00 PRIMER_LEFT_30_SELF_END=1.00 PRIMER_RIGHT_30_SELF_END=4.00 PRIMER_LEFT_30_END_STABILITY=7.6000 PRIMER_RIGHT_30_END_STABILITY=9.7000 PRIMER_PAIR_30_COMPL_ANY=4.00 PRIMER_PAIR_30_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_30=175 PRIMER_PAIR_PENALTY_31=1.3357 PRIMER_LEFT_31_PENALTY=1.026872 PRIMER_RIGHT_31_PENALTY=0.308871 PRIMER_LEFT_31_SEQUENCE=CCACGGATTATCCTTCCATTT PRIMER_RIGHT_31_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_31=85,21 PRIMER_RIGHT_31=217,20 PRIMER_LEFT_31_TM=60.027 PRIMER_RIGHT_31_TM=59.691 PRIMER_LEFT_31_GC_PERCENT=42.857 PRIMER_RIGHT_31_GC_PERCENT=55.000 PRIMER_LEFT_31_SELF_ANY=7.00 PRIMER_RIGHT_31_SELF_ANY=5.00 PRIMER_LEFT_31_SELF_END=0.00 PRIMER_RIGHT_31_SELF_END=1.00 PRIMER_LEFT_31_END_STABILITY=7.2000 PRIMER_RIGHT_31_END_STABILITY=9.4000 PRIMER_PAIR_31_COMPL_ANY=5.00 PRIMER_PAIR_31_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_31=133 PRIMER_PAIR_PENALTY_32=1.3393 PRIMER_LEFT_32_PENALTY=1.183236 PRIMER_RIGHT_32_PENALTY=0.156061 PRIMER_LEFT_32_SEQUENCE=TTCAGCTTTTTCCCCTTAGGA PRIMER_RIGHT_32_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_32=45,21 PRIMER_RIGHT_32=218,20 PRIMER_LEFT_32_TM=60.183 PRIMER_RIGHT_32_TM=59.844 PRIMER_LEFT_32_GC_PERCENT=42.857 PRIMER_RIGHT_32_GC_PERCENT=55.000 PRIMER_LEFT_32_SELF_ANY=5.00 PRIMER_RIGHT_32_SELF_ANY=5.00 PRIMER_LEFT_32_SELF_END=3.00 PRIMER_RIGHT_32_SELF_END=3.00 PRIMER_LEFT_32_END_STABILITY=7.2000 PRIMER_RIGHT_32_END_STABILITY=8.2000 PRIMER_PAIR_32_COMPL_ANY=4.00 PRIMER_PAIR_32_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_32=174 PRIMER_PAIR_PENALTY_33=1.3401 PRIMER_LEFT_33_PENALTY=1.184005 PRIMER_RIGHT_33_PENALTY=0.156061 PRIMER_LEFT_33_SEQUENCE=TTTCCCCTTAGGACCAAGGTA PRIMER_RIGHT_33_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_33=53,21 PRIMER_RIGHT_33=218,20 PRIMER_LEFT_33_TM=59.816 PRIMER_RIGHT_33_TM=59.844 PRIMER_LEFT_33_GC_PERCENT=47.619 PRIMER_RIGHT_33_GC_PERCENT=55.000 PRIMER_LEFT_33_SELF_ANY=7.00 PRIMER_RIGHT_33_SELF_ANY=5.00 PRIMER_LEFT_33_SELF_END=3.00 PRIMER_RIGHT_33_SELF_END=3.00 PRIMER_LEFT_33_END_STABILITY=6.9000 PRIMER_RIGHT_33_END_STABILITY=8.2000 PRIMER_PAIR_33_COMPL_ANY=5.00 PRIMER_PAIR_33_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_33=166 PRIMER_PAIR_PENALTY_34=1.3404 PRIMER_LEFT_34_PENALTY=1.031536 PRIMER_RIGHT_34_PENALTY=0.308871 PRIMER_LEFT_34_SEQUENCE=GACCACGGATTATCCTTCCAT PRIMER_RIGHT_34_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_34=83,21 PRIMER_RIGHT_34=217,20 PRIMER_LEFT_34_TM=60.032 PRIMER_RIGHT_34_TM=59.691 PRIMER_LEFT_34_GC_PERCENT=47.619 PRIMER_RIGHT_34_GC_PERCENT=55.000 PRIMER_LEFT_34_SELF_ANY=7.00 PRIMER_RIGHT_34_SELF_ANY=5.00 PRIMER_LEFT_34_SELF_END=2.00 PRIMER_RIGHT_34_SELF_END=1.00 PRIMER_LEFT_34_END_STABILITY=8.1000 PRIMER_RIGHT_34_END_STABILITY=9.4000 PRIMER_PAIR_34_COMPL_ANY=5.00 PRIMER_PAIR_34_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_34=135 PRIMER_PAIR_PENALTY_35=1.3437 PRIMER_LEFT_35_PENALTY=1.187663 PRIMER_RIGHT_35_PENALTY=0.156061 PRIMER_LEFT_35_SEQUENCE=GCCTTAGGACCACGGATTATC PRIMER_RIGHT_35_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_35=76,21 PRIMER_RIGHT_35=218,20 PRIMER_LEFT_35_TM=59.812 PRIMER_RIGHT_35_TM=59.844 PRIMER_LEFT_35_GC_PERCENT=52.381 PRIMER_RIGHT_35_GC_PERCENT=55.000 PRIMER_LEFT_35_SELF_ANY=5.00 PRIMER_RIGHT_35_SELF_ANY=5.00 PRIMER_LEFT_35_SELF_END=5.00 PRIMER_RIGHT_35_SELF_END=3.00 PRIMER_LEFT_35_END_STABILITY=5.9000 PRIMER_RIGHT_35_END_STABILITY=8.2000 PRIMER_PAIR_35_COMPL_ANY=4.00 PRIMER_PAIR_35_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_35=143 PRIMER_PAIR_PENALTY_36=1.3471 PRIMER_LEFT_36_PENALTY=1.329419 PRIMER_RIGHT_36_PENALTY=0.017711 PRIMER_LEFT_36_SEQUENCE=AGCCTTAGGACCACGGATTA PRIMER_RIGHT_36_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_36=75,20 PRIMER_RIGHT_36=220,20 PRIMER_LEFT_36_TM=58.671 PRIMER_RIGHT_36_TM=59.982 PRIMER_LEFT_36_GC_PERCENT=50.000 PRIMER_RIGHT_36_GC_PERCENT=55.000 PRIMER_LEFT_36_SELF_ANY=5.00 PRIMER_RIGHT_36_SELF_ANY=6.00 PRIMER_LEFT_36_SELF_END=2.00 PRIMER_RIGHT_36_SELF_END=4.00 PRIMER_LEFT_36_END_STABILITY=5.9000 PRIMER_RIGHT_36_END_STABILITY=9.7000 PRIMER_PAIR_36_COMPL_ANY=4.00 PRIMER_PAIR_36_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_36=146 PRIMER_PAIR_PENALTY_37=1.3471 PRIMER_LEFT_37_PENALTY=1.329419 PRIMER_RIGHT_37_PENALTY=0.017711 PRIMER_LEFT_37_SEQUENCE=TAGCCTTAGGACCACGGATT PRIMER_RIGHT_37_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_37=74,20 PRIMER_RIGHT_37=220,20 PRIMER_LEFT_37_TM=58.671 PRIMER_RIGHT_37_TM=59.982 PRIMER_LEFT_37_GC_PERCENT=50.000 PRIMER_RIGHT_37_GC_PERCENT=55.000 PRIMER_LEFT_37_SELF_ANY=5.00 PRIMER_RIGHT_37_SELF_ANY=6.00 PRIMER_LEFT_37_SELF_END=1.00 PRIMER_RIGHT_37_SELF_END=4.00 PRIMER_LEFT_37_END_STABILITY=8.1000 PRIMER_RIGHT_37_END_STABILITY=9.7000 PRIMER_PAIR_37_COMPL_ANY=4.00 PRIMER_PAIR_37_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_37=147 PRIMER_PAIR_PENALTY_38=1.4081 PRIMER_LEFT_38_PENALTY=1.099227 PRIMER_RIGHT_38_PENALTY=0.308871 PRIMER_LEFT_38_SEQUENCE=TCAGCTTTTTCCCCTTAGGA PRIMER_RIGHT_38_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_38=46,20 PRIMER_RIGHT_38=217,20 PRIMER_LEFT_38_TM=58.901 PRIMER_RIGHT_38_TM=59.691 PRIMER_LEFT_38_GC_PERCENT=45.000 PRIMER_RIGHT_38_GC_PERCENT=55.000 PRIMER_LEFT_38_SELF_ANY=5.00 PRIMER_RIGHT_38_SELF_ANY=5.00 PRIMER_LEFT_38_SELF_END=3.00 PRIMER_RIGHT_38_SELF_END=1.00 PRIMER_LEFT_38_END_STABILITY=7.2000 PRIMER_RIGHT_38_END_STABILITY=9.4000 PRIMER_PAIR_38_COMPL_ANY=4.00 PRIMER_PAIR_38_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_38=172 PRIMER_PAIR_PENALTY_39=1.4413 PRIMER_LEFT_39_PENALTY=1.285273 PRIMER_RIGHT_39_PENALTY=0.156061 PRIMER_LEFT_39_SEQUENCE=CCACGGATTATCCTTCCATT PRIMER_RIGHT_39_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_39=85,20 PRIMER_RIGHT_39=218,20 PRIMER_LEFT_39_TM=58.715 PRIMER_RIGHT_39_TM=59.844 PRIMER_LEFT_39_GC_PERCENT=45.000 PRIMER_RIGHT_39_GC_PERCENT=55.000 PRIMER_LEFT_39_SELF_ANY=7.00 PRIMER_RIGHT_39_SELF_ANY=5.00 PRIMER_LEFT_39_SELF_END=1.00 PRIMER_RIGHT_39_SELF_END=3.00 PRIMER_LEFT_39_END_STABILITY=8.4000 PRIMER_RIGHT_39_END_STABILITY=8.2000 PRIMER_PAIR_39_COMPL_ANY=5.00 PRIMER_PAIR_39_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_39=134 = PRIMER_NUM_RETURN=8 MARKER_NAME=09.RHAP33FA9.seq SEQUENCE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG INCLUDED_REGION=0,371 TARGET=151,18,AC TARGET=192,53,GA TARGET=248,16,AG PRIMER_PAIR_PENALTY=8.2998 PRIMER_LEFT_PENALTY=4.294975 PRIMER_RIGHT_PENALTY=4.004782 PRIMER_LEFT_SEQUENCE=TGAGACATCCTGTTACCATGTAGG PRIMER_RIGHT_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT=19,24 PRIMER_RIGHT=191,18 PRIMER_LEFT_TM=60.295 PRIMER_RIGHT_TM=62.005 PRIMER_LEFT_GC_PERCENT=45.833 PRIMER_RIGHT_GC_PERCENT=72.222 PRIMER_LEFT_SELF_ANY=4.00 PRIMER_RIGHT_SELF_ANY=1.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=0.00 PRIMER_LEFT_END_STABILITY=6.9000 PRIMER_RIGHT_END_STABILITY=9.4000 PRIMER_PAIR_MAX_COMPL_ANY=4.00 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_PRODUCT_SIZE=173 PRIMER_PAIR_PENALTY_1=8.3060 PRIMER_LEFT_1_PENALTY=4.301260 PRIMER_RIGHT_1_PENALTY=4.004782 PRIMER_LEFT_1_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCCA PRIMER_RIGHT_1_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_1=67,24 PRIMER_RIGHT_1=191,18 PRIMER_LEFT_1_TM=60.301 PRIMER_RIGHT_1_TM=62.005 PRIMER_LEFT_1_GC_PERCENT=37.500 PRIMER_RIGHT_1_GC_PERCENT=72.222 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_1=125 PRIMER_PAIR_PENALTY_2=8.5894 PRIMER_LEFT_2_PENALTY=4.584629 PRIMER_RIGHT_2_PENALTY=4.004782 PRIMER_LEFT_2_SEQUENCE=GAGACATCCTGTTACCATGTAGGA PRIMER_RIGHT_2_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_2=20,24 PRIMER_RIGHT_2=191,18 PRIMER_LEFT_2_TM=59.415 PRIMER_RIGHT_2_TM=62.005 PRIMER_LEFT_2_GC_PERCENT=45.833 PRIMER_RIGHT_2_GC_PERCENT=72.222 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_2=172 PRIMER_PAIR_PENALTY_3=8.9567 PRIMER_LEFT_3_PENALTY=4.951910 PRIMER_RIGHT_3_PENALTY=4.004782 PRIMER_LEFT_3_SEQUENCE=TTGAGACATCCTGTTACCATGT PRIMER_RIGHT_3_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_3=18,22 PRIMER_RIGHT_3=191,18 PRIMER_LEFT_3_TM=57.048 PRIMER_RIGHT_3_TM=62.005 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=72.222 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=1.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_3=174 PRIMER_PAIR_PENALTY_4=9.1359 PRIMER_LEFT_4_PENALTY=5.131103 PRIMER_RIGHT_4_PENALTY=4.004782 PRIMER_LEFT_4_SEQUENCE=AGAGAGGTTTCATTTTCTCTGCT PRIMER_RIGHT_4_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_4=48,23 PRIMER_RIGHT_4=191,18 PRIMER_LEFT_4_TM=57.869 PRIMER_RIGHT_4_TM=62.005 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=72.222 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_4=144 PRIMER_PAIR_PENALTY_5=9.2025 PRIMER_LEFT_5_PENALTY=5.197674 PRIMER_RIGHT_5_PENALTY=4.004782 PRIMER_LEFT_5_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCC PRIMER_RIGHT_5_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_5=67,23 PRIMER_RIGHT_5=191,18 PRIMER_LEFT_5_TM=57.802 PRIMER_RIGHT_5_TM=62.005 PRIMER_LEFT_5_GC_PERCENT=39.130 PRIMER_RIGHT_5_GC_PERCENT=72.222 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=1.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_END_STABILITY=9.7000 PRIMER_RIGHT_5_END_STABILITY=9.4000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_5=125 PRIMER_PAIR_PENALTY_6=9.2792 PRIMER_LEFT_6_PENALTY=5.274423 PRIMER_RIGHT_6_PENALTY=4.004782 PRIMER_LEFT_6_SEQUENCE=GACATCCTGTTACCATGTAGGAGTT PRIMER_RIGHT_6_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_6=22,25 PRIMER_RIGHT_6=191,18 PRIMER_LEFT_6_TM=59.726 PRIMER_RIGHT_6_TM=62.005 PRIMER_LEFT_6_GC_PERCENT=44.000 PRIMER_RIGHT_6_GC_PERCENT=72.222 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=1.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_END_STABILITY=6.4000 PRIMER_RIGHT_6_END_STABILITY=9.4000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_6=170 PRIMER_PAIR_PENALTY_7=9.2850 PRIMER_LEFT_7_PENALTY=5.280169 PRIMER_RIGHT_7_PENALTY=4.004782 PRIMER_LEFT_7_SEQUENCE=CTGCTAAGTAATGAGTTCAAAGCC PRIMER_RIGHT_7_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_7=66,24 PRIMER_RIGHT_7=191,18 PRIMER_LEFT_7_TM=58.720 PRIMER_RIGHT_7_TM=62.005 PRIMER_LEFT_7_GC_PERCENT=41.667 PRIMER_RIGHT_7_GC_PERCENT=72.222 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=1.00 PRIMER_LEFT_7_SELF_END=1.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=9.4000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_7=126 = MARKER_NAME=09.RHAP34FA9.seq SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT INCLUDED_REGION=20,280 TARGET=44,42,CA PRIMER_PAIR_PENALTY=7.2009 PRIMER_LEFT_PENALTY=6.971746 PRIMER_RIGHT_PENALTY=0.229181 PRIMER_LEFT_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_SEQUENCE=TTCCTCACAGAGGGAACAGG PRIMER_LEFT=20,24 PRIMER_RIGHT=166,20 PRIMER_LEFT_TM=57.028 PRIMER_RIGHT_TM=60.229 PRIMER_LEFT_GC_PERCENT=33.333 PRIMER_RIGHT_GC_PERCENT=55.000 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=5.00 PRIMER_LEFT_SELF_END=4.00 PRIMER_RIGHT_SELF_END=0.00 PRIMER_LEFT_END_STABILITY=5.8000 PRIMER_RIGHT_END_STABILITY=7.9000 PRIMER_PAIR_MAX_COMPL_ANY=4.00 PRIMER_PAIR_MAX_COMPL_END=0.00 PRIMER_PRODUCT_SIZE=147 PRIMER_PAIR_PENALTY_1=7.6879 PRIMER_LEFT_1_PENALTY=6.971746 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_LEFT_1_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_1_SEQUENCE=CAGGGGCTGTTTCTGATTCT PRIMER_LEFT_1=20,24 PRIMER_RIGHT_1=150,20 PRIMER_LEFT_1_TM=57.028 PRIMER_RIGHT_1_TM=59.284 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=5.8000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_1=131 PRIMER_PAIR_PENALTY_2=7.8310 PRIMER_LEFT_2_PENALTY=6.971746 PRIMER_RIGHT_2_PENALTY=0.859212 PRIMER_LEFT_2_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_2_SEQUENCE=ACAGGGGCTGTTTCTGATTC PRIMER_LEFT_2=20,24 PRIMER_RIGHT_2=151,20 PRIMER_LEFT_2_TM=57.028 PRIMER_RIGHT_2_TM=59.141 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_2=132 PRIMER_PAIR_PENALTY_3=7.8310 PRIMER_LEFT_3_PENALTY=6.971746 PRIMER_RIGHT_3_PENALTY=0.859212 PRIMER_LEFT_3_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_3_SEQUENCE=GAACAGGGGCTGTTTCTGAT PRIMER_LEFT_3=20,24 PRIMER_RIGHT_3=153,20 PRIMER_LEFT_3_TM=57.028 PRIMER_RIGHT_3_TM=59.141 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=5.8000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_3=134 PRIMER_PAIR_PENALTY_4=8.0391 PRIMER_LEFT_4_PENALTY=6.971746 PRIMER_RIGHT_4_PENALTY=1.067353 PRIMER_LEFT_4_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_4_SEQUENCE=GGGCTGTTTCTGATTCTTTCC PRIMER_LEFT_4=20,24 PRIMER_RIGHT_4=147,21 PRIMER_LEFT_4_TM=57.028 PRIMER_RIGHT_4_TM=60.067 PRIMER_LEFT_4_GC_PERCENT=33.333 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_4=128 PRIMER_PAIR_PENALTY_5=8.0391 PRIMER_LEFT_5_PENALTY=6.971746 PRIMER_RIGHT_5_PENALTY=1.067353 PRIMER_LEFT_5_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_5_SEQUENCE=GGGGCTGTTTCTGATTCTTTC PRIMER_LEFT_5=20,24 PRIMER_RIGHT_5=148,21 PRIMER_LEFT_5_TM=57.028 PRIMER_RIGHT_5_TM=60.067 PRIMER_LEFT_5_GC_PERCENT=33.333 PRIMER_RIGHT_5_GC_PERCENT=47.619 PRIMER_LEFT_5_SELF_ANY=6.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_LEFT_5_SELF_END=4.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_END_STABILITY=5.8000 PRIMER_RIGHT_5_END_STABILITY=7.0000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_5=129 PRIMER_PAIR_PENALTY_6=8.0963 PRIMER_LEFT_6_PENALTY=6.971746 PRIMER_RIGHT_6_PENALTY=1.124598 PRIMER_LEFT_6_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_6_SEQUENCE=ACAGGGGCTGTTTCTGATTCT PRIMER_LEFT_6=20,24 PRIMER_RIGHT_6=151,21 PRIMER_LEFT_6_TM=57.028 PRIMER_RIGHT_6_TM=60.125 PRIMER_LEFT_6_GC_PERCENT=33.333 PRIMER_RIGHT_6_GC_PERCENT=47.619 PRIMER_LEFT_6_SELF_ANY=6.00 PRIMER_RIGHT_6_SELF_ANY=5.00 PRIMER_LEFT_6_SELF_END=4.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_END_STABILITY=5.8000 PRIMER_RIGHT_6_END_STABILITY=6.6000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_6=132 PRIMER_PAIR_PENALTY_7=8.1993 PRIMER_LEFT_7_PENALTY=6.971746 PRIMER_RIGHT_7_PENALTY=1.227592 PRIMER_LEFT_7_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_7_SEQUENCE=TTTGTGTTGCTTGCATTTTTG PRIMER_LEFT_7=20,24 PRIMER_RIGHT_7=192,21 PRIMER_LEFT_7_TM=57.028 PRIMER_RIGHT_7_TM=59.772 PRIMER_LEFT_7_GC_PERCENT=33.333 PRIMER_RIGHT_7_GC_PERCENT=33.333 PRIMER_LEFT_7_SELF_ANY=6.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_LEFT_7_SELF_END=4.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=5.8000 PRIMER_RIGHT_7_END_STABILITY=7.6000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_7=173 = MARKER_NAME=10.RHAP34FA10.seq SEQUENCE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN INCLUDED_REGION=12,236 TARGET=155,32,TG PRIMER_PAIR_PENALTY=1.7506 PRIMER_LEFT_PENALTY=0.649665 PRIMER_RIGHT_PENALTY=1.100969 PRIMER_LEFT_SEQUENCE=CCTGCAATGGGATGAATACA PRIMER_RIGHT_SEQUENCE=CAAGGACAGAACTGAACTGGC PRIMER_LEFT=101,20 PRIMER_RIGHT=229,21 PRIMER_LEFT_TM=59.350 PRIMER_RIGHT_TM=59.899 PRIMER_LEFT_GC_PERCENT=45.000 PRIMER_RIGHT_GC_PERCENT=52.381 PRIMER_LEFT_SELF_ANY=4.00 PRIMER_RIGHT_SELF_ANY=4.00 PRIMER_LEFT_SELF_END=4.00 PRIMER_RIGHT_SELF_END=3.00 PRIMER_LEFT_END_STABILITY=5.6000 PRIMER_RIGHT_END_STABILITY=9.7000 PRIMER_PAIR_MAX_COMPL_ANY=3.00 PRIMER_PAIR_MAX_COMPL_END=0.00 PRIMER_PRODUCT_SIZE=129 PRIMER_PAIR_PENALTY_1=1.7506 PRIMER_LEFT_1_PENALTY=0.649665 PRIMER_RIGHT_1_PENALTY=1.100969 PRIMER_LEFT_1_SEQUENCE=CCTGCAATGGGATGAATACA PRIMER_RIGHT_1_SEQUENCE=GCAAGGACAGAACTGAACTGG PRIMER_LEFT_1=101,20 PRIMER_RIGHT_1=230,21 PRIMER_LEFT_1_TM=59.350 PRIMER_RIGHT_1_TM=59.899 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=5.6000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_1=130 PRIMER_PAIR_PENALTY_2=2.3642 PRIMER_LEFT_2_PENALTY=1.263200 PRIMER_RIGHT_2_PENALTY=1.100969 PRIMER_LEFT_2_SEQUENCE=GATATCCTGCAATGGGATGAA PRIMER_RIGHT_2_SEQUENCE=CAAGGACAGAACTGAACTGGC PRIMER_LEFT_2=96,21 PRIMER_RIGHT_2=229,21 PRIMER_LEFT_2_TM=59.737 PRIMER_RIGHT_2_TM=59.899 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_2=134 PRIMER_PAIR_PENALTY_3=2.3642 PRIMER_LEFT_3_PENALTY=1.263200 PRIMER_RIGHT_3_PENALTY=1.100969 PRIMER_LEFT_3_SEQUENCE=GATATCCTGCAATGGGATGAA PRIMER_RIGHT_3_SEQUENCE=GCAAGGACAGAACTGAACTGG PRIMER_LEFT_3=96,21 PRIMER_RIGHT_3=230,21 PRIMER_LEFT_3_TM=59.737 PRIMER_RIGHT_3_TM=59.899 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_3=135 PRIMER_PAIR_PENALTY_4=2.5784 PRIMER_LEFT_4_PENALTY=0.649665 PRIMER_RIGHT_4_PENALTY=1.928763 PRIMER_LEFT_4_SEQUENCE=CCTGCAATGGGATGAATACA PRIMER_RIGHT_4_SEQUENCE=TGCAAGGACAGAACTGAACTG PRIMER_LEFT_4=101,20 PRIMER_RIGHT_4=231,21 PRIMER_LEFT_4_TM=59.350 PRIMER_RIGHT_4_TM=59.071 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=5.6000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_4=131 PRIMER_PAIR_PENALTY_5=2.6739 PRIMER_LEFT_5_PENALTY=1.427458 PRIMER_RIGHT_5_PENALTY=1.246400 PRIMER_LEFT_5_SEQUENCE=TGGGGTTCAAAACAGAAAGAA PRIMER_RIGHT_5_SEQUENCE=GGCTCCTACAACCTGTCCTCT PRIMER_LEFT_5=37,21 PRIMER_RIGHT_5=211,21 PRIMER_LEFT_5_TM=59.573 PRIMER_RIGHT_5_TM=59.754 PRIMER_LEFT_5_GC_PERCENT=38.095 PRIMER_RIGHT_5_GC_PERCENT=57.143 PRIMER_LEFT_5_SELF_ANY=3.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_END_STABILITY=7.0000 PRIMER_RIGHT_5_END_STABILITY=7.9000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_5=175 PRIMER_PAIR_PENALTY_6=2.6834 PRIMER_LEFT_6_PENALTY=1.427458 PRIMER_RIGHT_6_PENALTY=1.255934 PRIMER_LEFT_6_SEQUENCE=TGGGGTTCAAAACAGAAAGAA PRIMER_RIGHT_6_SEQUENCE=GGCTCCTACAACCTGTCCTC PRIMER_LEFT_6=37,21 PRIMER_RIGHT_6=211,20 PRIMER_LEFT_6_TM=59.573 PRIMER_RIGHT_6_TM=58.744 PRIMER_LEFT_6_GC_PERCENT=38.095 PRIMER_RIGHT_6_GC_PERCENT=60.000 PRIMER_LEFT_6_SELF_ANY=3.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_END_STABILITY=7.0000 PRIMER_RIGHT_6_END_STABILITY=7.9000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_6=175 PRIMER_PAIR_PENALTY_7=2.7270 PRIMER_LEFT_7_PENALTY=0.649665 PRIMER_RIGHT_7_PENALTY=2.077340 PRIMER_LEFT_7_SEQUENCE=CCTGCAATGGGATGAATACA PRIMER_RIGHT_7_SEQUENCE=AAGGACAGAACTGAACTGGCTC PRIMER_LEFT_7=101,20 PRIMER_RIGHT_7=228,22 PRIMER_LEFT_7_TM=59.350 PRIMER_RIGHT_7_TM=59.923 PRIMER_LEFT_7_GC_PERCENT=45.000 PRIMER_RIGHT_7_GC_PERCENT=50.000 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_LEFT_7_SELF_END=4.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_END_STABILITY=5.6000 PRIMER_RIGHT_7_END_STABILITY=9.4000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_7=128 = MARKER_NAME=13.RHAP34FB1.seq SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN INCLUDED_REGION=21,244 TARGET=22,16,TA TARGET=57,34,GT = MARKER_NAME=14.RHAP34FB2.seq SEQUENCE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC INCLUDED_REGION=13,303 TARGET=177,33,AC TARGET=254,14,AC = primer3-2.3.6/test/primer3_config/0000775000077300007730000000000012231156372015744 5ustar ioanaioanaprimer3-2.3.6/test/primer3_config/loops.dh0000664000077300007730000000067112231156245017420 0ustar ioanaioana1 inf 0.0 inf 2 inf 0.0 inf 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 primer3-2.3.6/test/primer3_config/dangle.dh0000664000077300007730000000056512231156245017520 0ustar ioanaioana0 0 0 0 0 0 0 0 0 0 0 0 -500 4700 -4100 -3800 0 0 0 0 0 0 0 0 -5900 -2600 -3200 -5200 0 0 0 0 0 0 0 0 -2100 -200 -3900 -4400 0 0 0 0 0 0 0 0 -700 4400 -1600 2900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -2900 -4100 -4200 -200 0 0 0 0 0 0 0 0 -3700 -4000 -3900 -4900 0 0 0 0 0 0 0 0 -6300 -4400 -5100 -4000 0 0 0 0 0 0 0 0 200 600 -1100 -6900 0 0 0 0 0 0 0 0 0 0 0 0 primer3-2.3.6/test/primer3_config/tetraloop.dh0000664000077300007730000000162112231156245020271 0ustar ioanaioanaAAAAAT 500 AAAACT 700 AAACAT 1000 ACTTGT 0 AGAAAT -1100 AGAGAT -1100 AGATAT -1500 AGCAAT -1600 AGCGAT -1100 AGCTTT 200 AGGAAT -1100 AGGGAT -1100 AGGGGT 500 AGTAAT -1600 AGTGAT -1100 AGTTCT 800 ATTCGT -200 ATTTGT 0 ATTTTT -500 CAAAAG 500 CAAACG 700 CAACAG 1000 CAACCG 0 CCTTGG 0 CGAAAG -1100 CGAGAG -1100 CGATAG -1500 CGCAAG -1600 CGCGAG -1100 CGCTTG 200 CGGAAG -1100 CGGGAG -1000 CGGGGG 500 CGTAAG -1600 CGTGAG -1100 CGTTCG 800 CTTCGG -200 CTTTGG 0 CTTTTG -500 GAAAAC 500 GAAACC 700 GAACAC 1000 GCTTGC 0 GGAAAC -1100 GGAGAC -1100 GGATAC -1600 GGCAAC -1600 GGCGAC -1100 GGCTTC 200 GGGAAC -1100 GGGGAC -1100 GGGGGC 500 GGTAAC -1600 GGTGAC -1100 GGTTCC 800 GTTCGC -200 GTTTGC 0 GTTTTC -500 TAAAAA 500 TAAACA 700 TAACAA 1000 TCTTGA 0 TGAAAA -1100 TGAGAA -1100 TGATAA -1600 TGCAAA -1600 TGCGAA -1100 TGCTTA 200 TGGAAA -1100 TGGGAA -1100 TGGGGA 500 TGTAAA -1600 TGTGAA -1100 TGTTCA 800 TTTCGA -200 TTTTGA 0 TTTTTA -500 primer3-2.3.6/test/primer3_config/tstack.dh0000664000077300007730000000157212231156245017556 0ustar ioanaioana0 0 0 -2500 0 0 0 -2700 0 0 0 -2400 -3100 -1600 -1900 0 0 0 -8000 0 0 0 -3200 0 0 0 -4600 0 -1800 -100 0 -900 0 -4300 0 0 0 -2700 0 0 0 -6000 0 0 -2500 0 -1100 -3200 -3100 0 0 0 -1800 0 0 0 -2500 0 0 0 0 -2300 -3500 -2400 0 0 0 -2300 0 0 0 -700 -4300 -2600 -3900 0 0 0 0 -700 0 0 -5000 0 0 0 -3900 0 -2700 -2100 0 -3200 0 0 -3000 0 0 -2600 0 0 0 -2100 0 0 -6000 0 -3800 -3800 0 -3900 0 0 -1600 0 0 0 -100 0 0 0 0 -3900 -6600 -6100 -2300 0 0 0 0 0 0 -2000 -8000 -5000 -4300 0 0 0 0 -1100 0 0 0 -3600 0 0 -4300 0 -3200 -3900 0 -4900 0 0 -700 0 0 0 -5900 0 0 -3900 0 0 -4600 0 -700 -5700 0 -3800 0 0 0 -6600 0 0 -1900 0 0 0 0 -3000 -5900 -7400 -1100 0 0 0 -3500 0 0 0 -2500 -2300 -2000 -7200 0 0 0 -2500 0 0 0 -3900 0 0 0 -3200 -2700 -700 0 -2500 0 0 -4900 0 0 0 -5700 0 0 0 -7400 0 -2400 0 -1100 -3900 0 -3200 0 0 0 -3800 0 0 0 -6100 0 0 0 -700 -3600 -3200 -900 0 0 0 -3200 0 0 0 -2400 0 0 0 primer3-2.3.6/test/primer3_config/triloop.ds0000664000077300007730000000020012231156245017753 0ustar ioanaioanaAGAAT 0 AGCAT 0 AGGAT 0 AGTAT 0 CGAAG 0 CGCAG 0 CGGAG 0 CGTAG 0 GGAAC 0 GGCAC 0 GGGAC 0 GGTAC 0 TGAAA 0 TGCAA 0 TGGAA 0 TGTAA 0 primer3-2.3.6/test/primer3_config/tstack2.ds0000664000077300007730000000224012231156245017644 0ustar ioanaioanainf inf inf -6.3 inf inf inf -7.0 inf inf inf -5.8 -7.8 -4.0 -4.4 -13.2 inf inf -22.5 inf inf inf -7.1 inf inf inf -11.4 inf -3.8 -0.5 -16.1 -1.7 inf -10.7 inf inf inf -6.0 inf inf inf -15.5 inf inf -5.9 -16.1 -2.1 -8.7 -7.8 inf inf inf -3.8 inf inf inf -5.9 inf inf inf -13.6 -6.3 -9.4 -6.5 inf inf inf -5.9 inf inf inf -1.3 -10.7 -5.9 -9.6 -16.1 inf inf inf -1.2 inf inf -13.8 inf inf inf -10.6 inf -6.0 -5.1 -19.3 -8.0 inf inf -7.8 inf inf -5.9 inf inf inf -5.1 inf inf -15.5 -19.3 -9.5 -9.0 inf -10.6 inf inf -4.0 inf inf inf -0.5 inf inf inf -16.1 -10.6 -18.7 -16.9 -6.3 inf inf inf inf inf inf -4.7 -22.5 -13.8 -11.1 -16.1 inf inf inf -2.7 inf inf inf -9.8 inf inf -11.1 inf -7.1 -10.6 -19.3 -13.5 inf inf -19.2 inf inf inf -16.1 inf inf -9.6 inf inf -11.4 -19.3 -19.2 -15.9 inf -9.5 inf inf inf -18.7 inf inf -4.4 inf inf inf -16.1 -7.8 -16.1 -21.2 -2.1 inf inf inf -9.4 inf inf inf -6.3 -5.9 -4.7 -14.2 inf inf inf -6.3 inf inf inf -10.5 inf inf inf -8.9 -7.0 -1.3 -16.1 -6.3 inf inf -13.5 inf inf inf -15.9 inf inf inf -21.2 inf -5.8 -16.1 -2.7 -10.5 inf -8.0 inf inf inf -9.0 inf inf inf -16.9 inf inf -13.5 -1.2 -9.8 -8.9 -1.7 inf inf inf -8.7 inf inf inf -6.5 inf inf inf primer3-2.3.6/test/primer3_config/tetraloop.ds0000664000077300007730000000147012231156245020306 0ustar ioanaioanaAAAAAT -650 AAAACT 1610 AAACAT 1610 ACTTGT 4190 AGAAAT 1610 AGAGAT 1610 AGATAT 1610 AGCAAT 1610 AGCGAT 1610 AGCTTT 1610 AGGAAT 1610 AGGGAT 1610 AGGGGT 640 AGTAAT 1610 AGTGAT 1610 AGTTCT 1610 ATTCGT 1610 ATTTGT 1610 ATTTTT 1610 CAAAAG -1290 CAAACG 0 CAACAG 0 CAACCG 0 CCTTGG 2570 CGAAAG 0 CGAGAG 0 CGATAG 0 CGCAAG 0 CGCGAG 0 CGCTTG 0 CGGAAG 0 CGGGAG 0 CGGGGG -970 CGTAAG 0 CGTGAG 0 CGTTCG 0 CTTCGG 0 CTTTGG 0 CTTTTG 0 GAAAAC -3230 GAAACC 0 GAACAC 0 GCTTGC 2570 GGAAAC 0 GGAGAC 0 GGATAC 0 GGCAAC 0 GGCGAC 0 GGCTTC 0 GGGAAC 0 GGGGAC 0 GGGGGC -970 GGTAAC 0 GGTGAC 0 GGTTCC 0 GTTCGC 0 GTTTGC 0 GTTTTC 0 TAAAAA 320 TAAACA 1610 TAACAA 1610 TCTTGA 4190 TGAAAA 1610 TGAGAA 1610 TGATAA 1610 TGCAAA 1610 TGCGAA 1610 TGCTTA 1610 TGGAAA 1610 TGGGAA 1610 TGGGGA 640 TGTAAA 1610 TGTGAA 1610 TGTTCA 1610 TTTCGA 1610 TTTTGA 1610 TTTTTA 1610 primer3-2.3.6/test/primer3_config/dangle.ds0000664000077300007730000000104412231156245017524 0ustar ioanaioanainf inf inf inf inf inf inf inf inf inf inf inf -1.1 14.2 -13.1 -12.6 inf inf inf inf inf inf inf inf -16.5 -7.4 -10.4 -15 inf inf inf inf inf inf inf inf -3.9 -0.1 -11.2 -13.1 inf inf inf inf inf inf inf inf -0.8 14.9 -3.6 10.4 inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf -7.6 -13 -15 -0.5 inf inf inf inf inf inf inf inf -10 -11.9 -10.9 -13.8 inf inf inf inf inf inf inf inf -17.1 -12.6 -14 -10.9 inf inf inf inf inf inf inf inf 2.3 3.3 -1.6 -20 inf inf inf inf inf inf inf inf inf inf inf inf primer3-2.3.6/test/primer3_config/interpretations/0000775000077300007730000000000012231156374021200 5ustar ioanaioanaprimer3-2.3.6/test/primer3_config/interpretations/tstack_tm_inf_i.dh0000664000077300007730000000412712231156245024653 0ustar ioanaioanaAA_AA 0 AA_AC 0 AA_AG 0 AA_AT 25 AA_CA 0 AA_CC 0 AA_CG 0 AA_CT 27 AA_GA 0 AA_GC 0 AA_GG 0 AA_GT 24 AA_TA 31 AA_TC 16 AA_TG 19 AA_TT 0 AC_AA 0 AC_AC 0 AC_AG 80 AC_AT 0 AC_CA 0 AC_CC 0 AC_CG 32 AC_CT 0 AC_GA 0 AC_GC 0 AC_GG 46 AC_GT 0 AC_TA 18 AC_TC 1 AC_TG 0 AC_TT 9 AG_AA 0 AG_AC 43 AG_AG 0 AG_AT 0 AG_CA 0 AG_CC 27 AG_CG 0 AG_CT 0 AG_GA 0 AG_GC 60 AG_GG 0 AG_GT 0 AG_TA 25 AG_TC 0 AG_TG 11 AG_TT 32 AT_AA 31 AT_AC 0 AT_AG 0 AT_AT 0 AT_CA 18 AT_CC 0 AT_CG 0 AT_CT 0 AT_GA 25 AT_GC 0 AT_GG 0 AT_GT 0 AT_TA 0 AT_TC 23 AT_TG 35 AT_TT 24 CA_AA 0 CA_AC 0 CA_AG 0 CA_AT 23 CA_CA 0 CA_CC 0 CA_CG 0 CA_CT 7 CA_GA 43 CA_GC 26 CA_GG 39 CA_GT 0 CA_TA 0 CA_TC 0 CA_TG 0 CA_TT 7 CC_AA 0 CC_AC 0 CC_AG 50 CC_AT 0 CC_CA 0 CC_CC 0 CC_CG 39 CC_CT 0 CC_GA 27 CC_GC 21 CC_GG 0 CC_GT 32 CC_TA 0 CC_TC 0 CC_TG 30 CC_TT 0 CG_AA 0 CG_AC 26 CG_AG 0 CG_AT 0 CG_CA 0 CG_CC 21 CG_CG 0 CG_CT 0 CG_GA 60 CG_GC 0 CG_GG 38 CG_GT 38 CG_TA 0 CG_TC 39 CG_TG 0 CG_TT 0 CT_AA 16 CT_AC 0 CT_AG 0 CT_AT 0 CT_CA 1 CT_CC 0 CT_CG 0 CT_CT 0 CT_GA 0 CT_GC 39 CT_GG 66 CT_GT 61 CT_TA 23 CT_TC 0 CT_TG 0 CT_TT 0 GA_AA 0 GA_AC 0 GA_AG 0 GA_AT 20 GA_CA 80 GA_CC 50 GA_CG 43 GA_CT 0 GA_GA 0 GA_GC 0 GA_GG 0 GA_GT 11 GA_TA 0 GA_TC 0 GA_TG 0 GA_TT 36 GC_AA 0 GC_AC 0 GC_AG 43 GC_AT 0 GC_CA 32 GC_CC 39 GC_CG 0 GC_CT 49 GC_GA 0 GC_GC 0 GC_GG 7 GC_GT 0 GC_TA 0 GC_TC 0 GC_TG 59 GC_TT 0 GG_AA 0 GG_AC 39 GG_AG 0 GG_AT 0 GG_CA 46 GG_CC 0 GG_CG 7 GG_CT 57 GG_GA 0 GG_GC 38 GG_GG 0 GG_GT 0 GG_TA 0 GG_TC 66 GG_TG 0 GG_TT 0 GT_AA 19 GT_AC 0 GT_AG 0 GT_AT 0 GT_CA 0 GT_CC 30 GT_CG 59 GT_CT 74 GT_GA 11 GT_GC 0 GT_GG 0 GT_GT 0 GT_TA 35 GT_TC 0 GT_TG 0 GT_TT 0 TA_AA 25 TA_AC 23 TA_AG 20 TA_AT 72 TA_CA 0 TA_CC 0 TA_CG 0 TA_CT 25 TA_GA 0 TA_GC 0 TA_GG 0 TA_GT 39 TA_TA 0 TA_TC 0 TA_TG 0 TA_TT 32 TC_AA 27 TC_AC 7 TC_AG 0 TC_AT 25 TC_CA 0 TC_CC 0 TC_CG 49 TC_CT 0 TC_GA 0 TC_GC 0 TC_GG 57 TC_GT 0 TC_TA 0 TC_TC 0 TC_TG 74 TC_TT 0 TG_AA 24 TG_AC 0 TG_AG 11 TG_AT 39 TG_CA 0 TG_CC 32 TG_CG 0 TG_CT 0 TG_GA 0 TG_GC 38 TG_GG 0 TG_GT 0 TG_TA 0 TG_TC 61 TG_TG 0 TG_TT 0 TT_AA 0 TT_AC 7 TT_AG 36 TT_AT 32 TT_CA 9 TT_CC 0 TT_CG 0 TT_CT 0 TT_GA 32 TT_GC 0 TT_GG 0 TT_GT 0 TT_TA 24 TT_TC 0 TT_TG 0 TT_TT 0 primer3-2.3.6/test/primer3_config/interpretations/stackmm_i_mm.dh0000664000077300007730000000530512231156245024155 0ustar ioanaioana/* values in articles are modified as follows: dS *(-10) and dH *(-10) */ AA_AA inf AA_AC inf AA_AG inf AA_AT inf AA_CA inf AA_CC inf AA_CG inf AA_CT 7600 AA_GA inf AA_GC inf AA_GG inf AA_GT 3000 AA_TA 1200 AA_TC 2300 AA_TG -600 AA_TT inf AC_AA inf AC_AC inf AC_AG -2900 AC_AT inf AC_CA inf AC_CC inf AC_CG -700 AC_CT inf AC_GA inf AC_GC inf AC_GG 500 AC_GT inf AC_TA 5300 AC_TC 0 AC_TG -8400 AC_TT 700 AG_AA inf AG_AC -900 AG_AG inf AG_AT inf AG_CA inf AG_CC 600 AG_CG inf AG_CT inf AG_GA inf AG_GC -4000 AG_GG inf AG_GT inf AG_TA -700 AG_TC inf AG_TG -3100 AG_TT -1000 AT_AA 1200 AT_AC inf AT_AG inf AT_AT inf AT_CA 5300 AT_CC inf AT_CG inf AT_CT inf AT_GA -700 AT_GC inf AT_GG inf AT_GT inf AT_TA -7200 AT_TC -1200 AT_TG -2500 AT_TT -2700 CA_AA inf CA_AC inf CA_AG inf CA_AT 3400 CA_CA inf CA_CC inf CA_CG inf CA_CT 6100 CA_GA -900 CA_GC 1900 CA_GG -700 CA_GT inf CA_TA inf CA_TC inf CA_TG inf CA_TT 1000 CC_AA inf CC_AC inf CC_AG 5200 CC_AT inf CC_CA inf CC_CC inf CC_CG 3600 CC_CT inf CC_GA 600 CC_GC -1500 CC_GG inf CC_GT -800 CC_TA inf CC_TC inf CC_TG 5200 CC_TT inf CG_AA inf CG_AC 1900 CG_AG inf CG_AT inf CG_CA inf CG_CC -1500 CG_CG inf CG_CT inf CG_GA -4000 CG_GC inf CG_GG -4900 CG_GT -4100 CG_TA inf CG_TC -1500 CG_TG inf CG_TT inf CT_AA 2300 CT_AC inf CT_AG inf CT_AT inf CT_CA 0 CT_CC inf CT_CG inf CT_CT inf CT_GA inf CT_GC -1500 CT_GG -2800 CT_GT -5000 CT_TA -1200 CT_TC inf CT_TG inf CT_TT inf GA_AA inf GA_AC inf GA_AG inf GA_AT 700 GA_CA -2900 GA_CC 5200 GA_CG -600 GA_CT inf GA_GA inf GA_GC inf GA_GG inf GA_GT 1600 GA_TA inf GA_TC inf GA_TG inf GA_TT -1300 GC_AA inf GC_AC inf GC_AG -600 GC_AT inf GC_CA -700 GC_CC 3600 GC_CG inf GC_CT 2300 GC_GA inf GC_GC inf GC_GG -6000 GC_GT inf GC_TA inf GC_TC inf GC_TG -4400 GC_TT inf GG_AA inf GG_AC -700 GG_AG inf GG_AT inf GG_CA 500 GG_CC inf GG_CG -6000 GG_CT 3300 GG_GA inf GG_GC -4900 GG_GG inf GG_GT inf GG_TA inf GG_TC -2800 GG_TG inf GG_TT 5800 GT_AA -600 GT_AC inf GT_AG inf GT_AT inf GT_CA inf GT_CC 5200 GT_CG -4400 GT_CT -2200 GT_GA -3100 GT_GC inf GT_GG inf GT_GT inf GT_TA -2500 GT_TC inf GT_TG 4100 GT_TT inf TA_AA 4700 TA_AC 3400 TA_AG 700 TA_AT inf TA_CA inf TA_CC inf TA_CG inf TA_CT 1200 TA_GA inf TA_GC inf TA_GG inf TA_GT -100 TA_TA inf TA_TC inf TA_TG inf TA_TT 200 TC_AA 7600 TC_AC 6100 TC_AG inf TC_AT 1200 TC_CA inf TC_CC inf TC_CG 2300 TC_CT inf TC_GA inf TC_GC inf TC_GG 3300 TC_GT inf TC_TA inf TC_TC inf TC_TG -2200 TC_TT inf TG_AA 3000 TG_AC inf TG_AG 1600 TG_AT -100 TG_CA inf TG_CC -800 TG_CG inf TG_CT inf TG_GA inf TG_GC -4100 TG_GG inf TG_GT -1400 TG_TA inf TG_TC -5000 TG_TG inf TG_TT inf TT_AA inf TT_AC 1000 TT_AG -1300 TT_AT 200 TT_CA 700 TT_CC inf TT_CG inf TT_CT inf TT_GA -100 TT_GC inf TT_GG 5800 TT_GT inf TT_TA -2700 TT_TC inf TT_TG inf TT_TT inf primer3-2.3.6/test/primer3_config/interpretations/triloop_i.dh0000664000077300007730000000030212231156245023505 0ustar ioanaioana /* triloop bonus*/ /* Enthalpy Hairpin loop */ AGAAT 15 AGCAT 15 AGGAT 15 AGTAT 15 CGAAG 20 CGCAG 20 CGGAG 20 CGTAG 20 GGAAC 20 GGCAC 20 GGGAC 20 GGTAC 20 TGAAA 15 TGCAA 15 TGGAA 15 TGTAA 15 primer3-2.3.6/test/primer3_config/interpretations/triloop_i.ds0000664000077300007730000000024012231156245023521 0ustar ioanaioana/* bonuses for GNA triloops */ AGAAT 0 AGCAT 0 AGGAT 0 AGTAT 0 CGAAG 0 CGCAG 0 CGGAG 0 CGTAG 0 GGAAC 0 GGCAC 0 GGGAC 0 GGTAC 0 TGAAA 0 TGCAA 0 TGGAA 0 TGTAA 0 primer3-2.3.6/test/primer3_config/interpretations/dangle_i.dh0000664000077300007730000000174212231156245023260 0ustar ioanaioana/* Enthalpy of dangling end */ /* 3' dangling */ AA_A 0 AC_A 0 AG_A 0 AT_A 0 AA_C 0 AC_C 0 AG_C 0 AT_C 0 AA_G 0 AC_G 0 AG_G 0 AT_G 0 AA_T 5 AC_T -47 AG_T 41 AT_T 38 CA_A 0 CC_A 0 CG_A 0 CT_A 0 CA_C 0 CC_C 0 CG_C 0 CT_C 0 CA_G 59 CC_G 26 CG_G 32 CT_G 52 CA_T 0 CC_T 0 CG_T 0 CT_T 0 GA_A 0 GC_A 0 GG_A 0 GT_A 0 GA_C 21 GC_C 2 GG_C 39 GT_C 44 GA_G 0 GC_G 0 GG_G 0 GT_G 0 GA_T 0 GC_T 0 GG_T 0 GT_T 0 TA_A 7 TC_A -44 TG_A 16 TT_A -29 TA_C 0 TC_C 0 TG_C 0 TT_C 0 TA_G 0 TC_G 0 TG_G 0 TT_G 0 TA_T 0 TC_T 0 TG_T 0 TT_T 0 /* 5' dangling */ A_AA 0 A_AC 0 A_AG 0 A_AT 0 A_CA 0 A_CC 0 A_CG 0 A_CT 0 A_GA 0 A_GC 0 A_GG 0 A_GT 0 A_TA 29 A_TC 41 A_TG 42 A_TT 2 C_AA 0 C_AC 0 C_AG 0 C_AT 0 C_CA 0 C_CC 0 C_CG 0 C_CT 0 C_GA 37 C_GC 40 C_GG 39 C_GT 49 C_TA 0 C_TC 0 C_TG 0 C_TT 0 G_AA 0 G_AC 0 G_AG 0 G_AT 0 G_CA 63 G_CC 44 G_CG 51 G_CT 40 G_GA 0 G_GC 0 G_GG 0 G_GT 0 G_TA 0 G_TC 0 G_TG 0 G_TT 0 T_AA -2 T_AC -6 T_AG 11 T_AT 69 T_CA 0 T_CC 0 T_CG 0 T_CT 0 T_GA 0 T_GC 0 T_GG 0 T_GT 0 T_TA 0 T_TC 0 T_TG 0 T_TT 0 primer3-2.3.6/test/primer3_config/interpretations/tetraloop_i.dh0000664000077300007730000000145712231156245024042 0ustar ioanaioana/* tetraloop bonuses for GNRA and GNAB kcal/mol*(-10) */ AAAAAT -5 AAAACT -7 AAACAT -10 ACTTGT 0 AGAAAT 11 AGAGAT 11 AGATAT 15 AGCAAT 16 AGCGAT 11 AGCTTT -2 AGGAAT 11 AGGGAT 11 AGGGGT -5 AGTAAT 16 AGTGAT 11 AGTTCT -8 ATTCGT 2 ATTTGT 0 ATTTTT 5 CAAAAG -5 CAAACG -7 CAACAG -10 CAACCG 0 CCTTGG 0 CGAAAG 11 CGAGAG 11 CGATAG 15 CGCAAG 16 CGCGAG 11 CGCTTG -2 CGGAAG 11 CGGGAG 10 CGGGGG -5 CGTAAG 16 CGTGAG 11 CGTTCG -8 CTTCGG 2 CTTTGG 0 CTTTTG 5 GAAAAC -5 GAAACC -7 GAACAC -10 GCTTGC 0 GGAAAC 11 GGAGAC 11 GGATAC 16 GGCAAC 16 GGCGAC 11 GGCTTC -2 GGGAAC 11 GGGGAC 11 GGGGGC -5 GGTAAC 16 GGTGAC 11 GGTTCC -8 GTTCGC 2 GTTTGC 0 GTTTTC 5 TAAAAA -5 TAAACA -7 TAACAA -10 TCTTGA 0 TGAAAA 11 TGAGAA 11 TGATAA 16 TGCAAA 16 TGCGAA 11 TGCTTA -2 TGGAAA 11 TGGGAA 11 TGGGGA -5 TGTAAA 16 TGTGAA 11 TGTTCA -8 TTTCGA 2 TTTTGA 0 TTTTTA 5 primer3-2.3.6/test/primer3_config/interpretations/stackmm_i_mm.ds0000664000077300007730000000512312231156245024166 0ustar ioanaioanaAA_AA inf AA_AC inf AA_AG inf AA_AT 12.9 AA_CA inf AA_CC inf AA_CG inf AA_CT 20.2 AA_GA inf AA_GC inf AA_GG inf AA_GT 7.4 AA_TA 1.7 AA_TC 4.6 AA_TG -2.3 AA_TT inf AC_AA inf AC_AC inf AC_AG -9.8 AC_AT inf AC_CA inf AC_CC inf AC_CG -3.8 AC_CT inf AC_GA inf AC_GC inf AC_GG 3.2 AC_GT inf AC_TA 14.6 AC_TC -4.4 AC_TG inf AC_TT 0.2 AG_AA inf AG_AC -4.2 AG_AG inf AG_AT inf AG_CA inf AG_CC 0.6 AG_CG inf AG_CT inf AG_GA inf AG_GC -13.2 AG_GG inf AG_GT inf AG_TA -2.3 AG_TC inf AG_TG -9.5 AG_TT 0.9 AT_AA 1.7 AT_AC inf AT_AG inf AT_AT inf AT_CA 14.6 AT_CC inf AT_CG inf AT_CT inf AT_GA -2.3 AT_GC inf AT_GG inf AT_GT inf AT_TA inf AT_TC -6.2 AT_TG -8.3 AT_TT -10.8 CA_AA inf CA_AC inf CA_AG inf CA_AT 8.0 CA_CA inf CA_CC inf CA_CG inf CA_CT 16.4 CA_GA -4.2 CA_GC 3.7 CA_GG -2.3 CA_GT inf CA_TA inf CA_TC inf CA_TG inf CA_TT 0.7 CC_AA inf CC_AC inf CC_AG 14.2 CC_AT inf CC_CA inf CC_CC inf CC_CG 8.9 CC_CT inf CC_GA -0.6 CC_GC -7.2 CC_GG -19.9 CC_GT -4.5 CC_TA inf CC_TC inf CC_TG 13.5 CC_TT inf CG_AA inf CG_AC 3.7 CG_AG inf CG_AT inf CG_CA inf CG_CC -7.2 CG_CG inf CG_CT inf CG_GA -13.2 CG_GC inf CG_GG -15.3 CG_GT -11.7 CG_TA inf CG_TC -6.1 CG_TG inf CG_TT inf CT_AA 4.6 CT_AC inf CT_AG inf CT_AT inf CT_CA -4.4 CT_CC inf CT_CG inf CT_CT inf CT_GA inf CT_GC -6.1 CT_GG -8.0 CT_GT -15.8 CT_TA -6.2 CT_TC inf CT_TG inf CT_TT inf GA_AA inf GA_AC inf GA_AG inf GA_AT 0.7 GA_CA -9.8 GA_CC 14.2 GA_CG -1.0 GA_CT inf GA_GA inf GA_GC inf GA_GG inf GA_GT 3.6 GA_TA inf GA_TC inf GA_TG inf GA_TT -5.3 GC_AA inf GC_AC inf GC_AG -1.0 GC_AT inf GC_CA -3.8 GC_CC 8.9 GC_CG inf GC_CT 5.4 GC_GA inf GC_GC inf GC_GG -15.8 GC_GT inf GC_TA inf GC_TC inf GC_TG -12.3 GC_TT inf GG_AA inf GG_AC -2.3 GG_AG inf GG_AT inf GG_CA 3.2 GG_CC -19.9 GG_CG -15.8 GG_CT 10.4 GG_GA inf GG_GC -15.3 GG_GG inf GG_GT inf GG_TA inf GG_TC 80 GG_TG inf GG_TT 16.3 GT_AA 23 GT_AC inf GT_AG inf GT_AT inf GT_CA inf GT_CC 13.5 GT_CG -12.3 GT_CT -8.4 GT_GA -9.5 GT_GC inf GT_GG inf GT_GT inf GT_TA -8.3 GT_TC inf GT_TG 9.5 GT_TT inf TA_AA 12.9 TA_AC 8.0 TA_AG 0.7 TA_AT inf TA_CA inf TA_CC inf TA_CG inf TA_CT 0.7 TA_GA inf TA_GC inf TA_GG inf TA_GT -1.7 TA_TA inf TA_TC inf TA_TG inf TA_TT -1.5 TC_AA 20.2 TC_AC 16.4 TC_AG inf TC_AT 0.7 TC_CA inf TC_CC inf TC_CG 5.4 TC_CT inf TC_GA inf TC_GC inf TC_GG 10.4 TC_GT inf TC_TA inf TC_TC inf TC_TG -8.4 TC_TT inf TG_AA 7.4 TG_AC inf TG_AG 3.6 TG_AT -1.7 TG_CA inf TG_CC -4.5 TG_CG inf TG_CT inf TG_GA inf TG_GC -11.7 TG_GG inf TG_GT -6.2 TG_TA inf TG_TC -15.8 TG_TG inf TG_TT inf TT_AA inf TT_AC -7 TT_AG -5.3 TT_AT -1.5 TT_CA 0.2 TT_CC inf TT_CG inf TT_CT inf TT_GA 0.9 TT_GC inf TT_GG 16.3 TT_GT inf TT_TA -10.8 TT_TC inf TT_TG inf TT_TT inf primer3-2.3.6/test/primer3_config/interpretations/tstack_tm_inf_i.ds0000664000077300007730000000467612231156245024677 0ustar ioanaioanaAA_AA inf AA_AC inf AA_AG inf AA_AT 63 AA_CA inf AA_CC inf AA_CG inf AA_CT 70 AA_GA inf AA_GC inf AA_GG inf AA_GT 58 AA_TA 78 AA_TC 40 AA_TG 44 AA_TT inf AC_AA inf AC_AC inf AC_AG 225 AC_AT inf AC_CA inf AC_CC inf AC_CG 71 AC_CT inf AC_GA inf AC_GC inf AC_GG 114 AC_GT inf AC_TA 38 AC_TC 5 AC_TG inf AC_TT 17 AG_AA inf AG_AC 107 AG_AG inf AG_AT inf AG_CA inf AG_CC 60 AG_CG inf AG_CT inf AG_GA inf AG_GC 155 AG_GG inf AG_GT inf AG_TA 59 AG_TC inf AG_TG 21 AG_TT 87 AT_AA 78 AT_AC inf AT_AG inf AT_AT inf AT_CA 38 AT_CC inf AT_CG inf AT_CT inf AT_GA 59 AT_GC inf AT_GG inf AT_GT inf AT_TA inf AT_TC 63 AT_TG 94 AT_TT 65 CA_AA inf CA_AC inf CA_AG inf CA_AT 59 CA_CA inf CA_CC inf CA_CG inf CA_CT 13 CA_GA 107 CA_GC 59 CA_GG 96 CA_GT inf CA_TA inf CA_TC inf CA_TG inf CA_TT 12 CC_AA inf CC_AC inf CC_AG 138 CC_AT inf CC_CA inf CC_CC inf CC_CG 106 CC_CT inf CC_GA 60 CC_GC 51 CC_GG inf CC_GT 80 CC_TA inf CC_TC inf CC_TG 78 CC_TT inf CG_AA inf CG_AC 59 CG_AG inf CG_AT inf CG_CA inf CG_CC 51 CG_CG inf CG_CT inf CG_GA 155 CG_GC inf CG_GG 95 CG_GT 90 CG_TA inf CG_TC 106 CG_TG inf CG_TT inf CT_AA 40 CT_AC inf CT_AG inf CT_AT inf CT_CA 5 CT_CC inf CT_CG inf CT_CT inf CT_GA inf CT_GC 106 CT_GG 187 CT_GT 169 CT_TA 63 CT_TC inf CT_TG inf CT_TT inf GA_AA inf GA_AC inf GA_AG inf GA_AT 47 GA_CA 225 GA_CC 138 GA_CG 111 GA_CT inf GA_GA inf GA_GC inf GA_GG inf GA_GT 27 GA_TA inf GA_TC inf GA_TG inf GA_TT 98 GC_AA inf GC_AC inf GC_AG 111 GC_AT inf GC_CA 71 GC_CC 106 GC_CG inf GC_CT 135 GC_GA inf GC_GC inf GC_GG 192 GC_GT inf GC_TA inf GC_TC inf GC_TG 161 GC_TT inf GG_AA inf GG_AC 96 GG_AG inf GG_AT inf GG_CA 114 GG_CC inf GG_CG 192 GG_CT 159 GG_GA inf GG_GC 95 GG_GG inf GG_GT inf GG_TA inf GG_TC 187 GG_TG inf GG_TT inf GT_AA 44 GT_AC inf GT_AG inf GT_AT inf GT_CA inf GT_CC 78 GT_CG 161 GT_CT 212 GT_GA 21 GT_GC inf GT_GG inf GT_GT inf GT_TA 94 GT_TC inf GT_TG inf GT_TT inf TA_AA 63 TA_AC 59 TA_AG 47 TA_AT inf TA_CA inf TA_CC inf TA_CG inf TA_CT 63 TA_GA inf TA_GC inf TA_GG inf TA_GT 105 TA_TA inf TA_TC inf TA_TG inf TA_TT 89 TC_AA 70 TC_AC 13 TC_AG inf TC_AT 63 TC_CA inf TC_CC inf TC_CG 135 TC_CT inf TC_GA inf TC_GC inf TC_GG 159 TC_GT inf TC_TA inf TC_TC inf TC_TG 212 TC_TT inf TG_AA 58 TG_AC inf TG_AG 27 TG_AT 105 TG_CA inf TG_CC 80 TG_CG inf TG_CT inf TG_GA inf TG_GC 90 TG_GG inf TG_GT inf TG_TA inf TG_TC 169 TG_TG inf TG_TT inf TT_AA inf TT_AC 12 TT_AG 98 TT_AT 89 TT_CA 17 TT_CC inf TT_CG inf TT_CT inf TT_GA 87 TT_GC inf TT_GG inf TT_GT inf TT_TA 65 TT_TC inf TT_TG inf TT_TT inf primer3-2.3.6/test/primer3_config/interpretations/stack_i.dh0000664000077300007730000000515312231156245023133 0ustar ioanaioana/* values in articles are modified as follows: dS *(-10) and dH *(-10) */ AA_AA inf AA_AC inf AA_AG inf AA_AT inf AA_CA inf AA_CC inf AA_CG inf AA_CT inf AA_GA inf AA_GC inf AA_GG inf AA_GT inf AA_TA inf AA_TC inf AA_TG inf AA_TT -7900 AC_AA inf AC_AC inf AC_AG inf AC_AT inf AC_CA inf AC_CC inf AC_CG inf AC_CT inf AC_GA inf AC_GC inf AC_GG inf AC_GT inf AC_TA inf AC_TC inf AC_TG -8400 AC_TT inf AG_AA inf AG_AC inf AG_AG inf AG_AT inf AG_CA inf AG_CC inf AG_CG inf AG_CT inf AG_GA inf AG_GC inf AG_GG inf AG_GT inf AG_TA inf AG_TC -7800 AG_TG inf AG_TT inf AT_AA inf AT_AC inf AT_AG inf AT_AT inf AT_CA inf AT_CC inf AT_CG inf AT_CT inf AT_GA inf AT_GC inf AT_GG inf AT_GT inf AT_TA -7200 AT_TC inf AT_TG inf AT_TT inf CA_AA inf CA_AC inf CA_AG inf CA_AT inf CA_CA inf CA_CC inf CA_CG inf CA_CT inf CA_GA inf CA_GC inf CA_GG inf CA_GT -8500 CA_TA inf CA_TC inf CA_TG inf CA_TT inf CC_AA inf CC_AC inf CC_AG -52 CC_AT inf CC_CA inf CC_CC inf CC_CG -36 CC_CT inf CC_GA inf CC_GC inf CC_GG -8000 CC_GT inf CC_TA inf CC_TC inf CC_TG inf CC_TT inf CG_AA inf CG_AC inf CG_AG inf CG_AT inf CG_CA inf CG_CC inf CG_CG inf CG_CT inf CG_GA inf CG_GC -10600 CG_GG inf CG_GT inf CG_TA inf CG_TC inf CG_TG inf CG_TT inf CT_AA inf CT_AC inf CT_AG inf CT_AT inf CT_CA inf CT_CC inf CT_CG inf CT_CT inf CT_GA -7800 CT_GC inf CT_GG inf CT_GT inf CT_TA inf CT_TC inf CT_TG inf CT_TT inf GA_AA inf GA_AC inf GA_AG inf GA_AT inf GA_CA inf GA_CC inf GA_CG inf GA_CT -8200 GA_GA inf GA_GC inf GA_GG inf GA_GT inf GA_TA inf GA_TC inf GA_TG inf GA_TT inf GC_AA inf GC_AC inf GC_AG inf GC_AT inf GC_CA inf GC_CC inf GC_CG -9800 GC_CT inf GC_GA inf GC_GC inf GC_GG inf GC_GT inf GC_TA inf GC_TC inf GC_TG inf GC_TT inf GG_AA inf GG_AC inf GG_AG inf GG_AT inf GG_CA inf GG_CC -8000 GG_CG inf GG_CT inf GG_GA inf GG_GC inf GG_GG inf GG_GT inf GG_TA inf GG_TC inf GG_TG inf GG_TT inf GT_AA inf GT_AC inf GT_AG inf GT_AT inf GT_CA -8400 GT_CC inf GT_CG inf GT_CT inf GT_GA inf GT_GC inf GT_GG inf GT_GT inf GT_TA inf GT_TC inf GT_TG inf GT_TT inf TA_AA inf TA_AC inf TA_AG inf TA_AT -7200 TA_CA inf TA_CC inf TA_CG inf TA_CT inf TA_GA inf TA_GC inf TA_GG inf TA_GT inf TA_TA inf TA_TC inf TA_TG inf TA_TT inf TC_AA inf TC_AC inf TC_AG -8200 TC_AT inf TC_CA inf TC_CC inf TC_CG inf TC_CT inf TC_GA inf TC_GC inf TC_GG inf TC_GT inf TC_TA inf TC_TC inf TC_TG inf TC_TT inf TG_AA inf TG_AC -8500 TG_AG inf TG_AT inf TG_CA inf TG_CC inf TG_CG inf TG_CT inf TG_GA inf TG_GC inf TG_GG inf TG_GT inf TG_TA inf TG_TC inf TG_TG inf TG_TT inf TT_AA -7900 TT_AC inf TT_AG inf TT_AT inf TT_CA inf TT_CC inf TT_CG inf TT_CT inf TT_GA inf TT_GC inf TT_GG inf TT_GT inf TT_TA inf TT_TC inf TT_TG inf TT_TT inf primer3-2.3.6/test/primer3_config/interpretations/tstack_i.ds0000664000077300007730000000463712231156245023340 0ustar ioanaioanaAA_AA inf AA_AC inf AA_AG inf AA_AT 63 AA_CA inf AA_CC inf AA_CG inf AA_CT 70 AA_GA inf AA_GC inf AA_GG inf AA_GT 58 AA_TA 78 AA_TC 40 AA_TG 44 AA_TT 0 AC_AA inf AC_AC inf AC_AG 225 AC_AT inf AC_CA inf AC_CC inf AC_CG 71 AC_CT inf AC_GA inf AC_GC inf AC_GG 114 AC_GT inf AC_TA 38 AC_TC 5 AC_TG 0 AC_TT 17 AG_AA inf AG_AC 107 AG_AG inf AG_AT inf AG_CA inf AG_CC 60 AG_CG inf AG_CT inf AG_GA inf AG_GC 155 AG_GG inf AG_GT inf AG_TA 59 AG_TC 0 AG_TG 21 AG_TT 87 AT_AA 78 AT_AC inf AT_AG inf AT_AT inf AT_CA 38 AT_CC inf AT_CG inf AT_CT inf AT_GA 59 AT_GC inf AT_GG inf AT_GT inf AT_TA 0 AT_TC 63 AT_TG 94 AT_TT 65 CA_AA inf CA_AC inf CA_AG inf CA_AT 59 CA_CA inf CA_CC inf CA_CG inf CA_CT 13 CA_GA 107 CA_GC 59 CA_GG 96 CA_GT 0 CA_TA inf CA_TC inf CA_TG inf CA_TT 12 CC_AA inf CC_AC inf CC_AG 138 CC_AT inf CC_CA inf CC_CC inf CC_CG 106 CC_CT inf CC_GA 60 CC_GC 51 CC_GG 0 CC_GT 80 CC_TA inf CC_TC inf CC_TG 78 CC_TT inf CG_AA inf CG_AC 59 CG_AG inf CG_AT inf CG_CA inf CG_CC 51 CG_CG inf CG_CT inf CG_GA 155 CG_GC 0 CG_GG 95 CG_GT 90 CG_TA inf CG_TC 106 CG_TG inf CG_TT inf CT_AA 40 CT_AC inf CT_AG inf CT_AT inf CT_CA 5 CT_CC inf CT_CG inf CT_CT inf CT_GA 0 CT_GC 106 CT_GG 187 CT_GT 169 CT_TA 63 CT_TC inf CT_TG inf CT_TT inf GA_AA inf GA_AC inf GA_AG inf GA_AT 47 GA_CA 225 GA_CC 138 GA_CG 111 GA_CT 0 GA_GA inf GA_GC inf GA_GG inf GA_GT 27 GA_TA inf GA_TC inf GA_TG inf GA_TT 98 GC_AA inf GC_AC inf GC_AG 111 GC_AT inf GC_CA 71 GC_CC 106 GC_CG 0 GC_CT 135 GC_GA inf GC_GC inf GC_GG 192 GC_GT inf GC_TA inf GC_TC inf GC_TG 161 GC_TT inf GG_AA inf GG_AC 96 GG_AG inf GG_AT inf GG_CA 114 GG_CC 0 GG_CG 192 GG_CT 159 GG_GA inf GG_GC 95 GG_GG inf GG_GT inf GG_TA inf GG_TC 187 GG_TG inf GG_TT inf GT_AA 44 GT_AC inf GT_AG inf GT_AT inf GT_CA 0 GT_CC 78 GT_CG 161 GT_CT 212 GT_GA 21 GT_GC inf GT_GG inf GT_GT inf GT_TA 94 GT_TC inf GT_TG inf GT_TT inf TA_AA 63 TA_AC 59 TA_AG 47 TA_AT 0 TA_CA inf TA_CC inf TA_CG inf TA_CT 63 TA_GA inf TA_GC inf TA_GG inf TA_GT 105 TA_TA inf TA_TC inf TA_TG inf TA_TT 89 TC_AA 70 TC_AC 13 TC_AG 0 TC_AT 63 TC_CA inf TC_CC inf TC_CG 135 TC_CT inf TC_GA inf TC_GC inf TC_GG 159 TC_GT inf TC_TA inf TC_TC inf TC_TG 212 TC_TT inf TG_AA 58 TG_AC 0 TG_AG 27 TG_AT 105 TG_CA inf TG_CC 80 TG_CG inf TG_CT inf TG_GA inf TG_GC 90 TG_GG inf TG_GT inf TG_TA inf TG_TC 169 TG_TG inf TG_TT inf TT_AA 0 TT_AC 12 TT_AG 98 TT_AT 89 TT_CA 17 TT_CC inf TT_CG inf TT_CT inf TT_GA 87 TT_GC inf TT_GG inf TT_GT inf TT_TA 65 TT_TC inf TT_TG inf TT_TT inf primer3-2.3.6/test/primer3_config/interpretations/tstack2_i.ds0000664000077300007730000000524012231156245023411 0ustar ioanaioanaAA_AA inf AA_AC inf AA_AG inf AA_AT -6.3 AA_CA inf AA_CC inf AA_CG inf AA_CT -7.0 AA_GA inf AA_GC inf AA_GG inf AA_GT -5.8 AA_TA -7.8 AA_TC -4.0 AA_TG -4.4 AA_TT -13.2 AC_AA inf AC_AC inf AC_AG -22.5 AC_AT inf AC_CA inf AC_CC inf AC_CG -7.1 AC_CT inf AC_GA inf AC_GC inf AC_GG -11.4 AC_GT inf AC_TA -3.8 AC_TC -0.5 AC_TG -16.1 AC_TT -1.7 AG_AA inf AG_AC -10.7 AG_AG inf AG_AT inf AG_CA inf AG_CC -6.0 AG_CG inf AG_CT inf AG_GA inf AG_GC -15.5 AG_GG inf AG_GT inf AG_TA -5.9 AG_TC -16.1 AG_TG -2.1 AG_TT -8.7 AT_AA -7.8 AT_AC inf AT_AG inf AT_AT inf AT_CA -3.8 AT_CC inf AT_CG inf AT_CT inf AT_GA -5.9 AT_GC inf AT_GG inf AT_GT inf AT_TA -13.6 AT_TC -6.3 AT_TG -9.4 AT_TT -6.5 CA_AA inf CA_AC inf CA_AG inf CA_AT -5.9 CA_CA inf CA_CC inf CA_CG inf CA_CT -1.3 CA_GA -10.7 CA_GC -5.9 CA_GG -9.6 CA_GT -16.1 CA_TA inf CA_TC inf CA_TG inf CA_TT -1.2 CC_AA inf CC_AC inf CC_AG -13.8 CC_AT inf CC_CA inf CC_CC inf CC_CG -10.6 CC_CT inf CC_GA -6.0 CC_GC -5.1 CC_GG -19.3 CC_GT -8.0 CC_TA inf CC_TC inf CC_TG -7.8 CC_TT inf CG_AA inf CG_AC -5.9 CG_AG inf CG_AT inf CG_CA inf CG_CC -5.1 CG_CG inf CG_CT inf CG_GA -15.5 CG_GC -19.3 CG_GG -9.5 CG_GT -9.0 CG_TA inf CG_TC -10.6 CG_TG inf CG_TT inf CT_AA -4.0 CT_AC inf CT_AG inf CT_AT inf CT_CA -0.5 CT_CC inf CT_CG inf CT_CT inf CT_GA -16.1 CT_GC -10.6 CT_GG -18.7 CT_GT -16.9 CT_TA -6.3 CT_TC inf CT_TG inf CT_TT inf GA_AA inf GA_AC inf GA_AG inf GA_AT -4.7 GA_CA -22.5 GA_CC -13.8 GA_CG -11.1 GA_CT -16.1 GA_GA inf GA_GC inf GA_GG inf GA_GT -2.7 GA_TA inf GA_TC inf GA_TG inf GA_TT -9.8 GC_AA inf GC_AC inf GC_AG -11.1 GC_AT inf GC_CA -7.1 GC_CC -10.6 GC_CG -19.3 GC_CT -13.5 GC_GA inf GC_GC inf GC_GG -19.2 GC_GT inf GC_TA inf GC_TC inf GC_TG -16.1 GC_TT inf GG_AA inf GG_AC -9.6 GG_AG inf GG_AT inf GG_CA -11.4 GG_CC -19.3 GG_CG -19.2 GG_CT -15.9 GG_GA inf GG_GC -9.5 GG_GG inf GG_GT inf GG_TA inf GG_TC -18.7 GG_TG inf GG_TT inf GT_AA -4.4 GT_AC inf GT_AG inf GT_AT inf GT_CA -16.1 GT_CC -7.8 GT_CG -16.1 GT_CT -21.2 GT_GA -2.1 GT_GC inf GT_GG inf GT_GT inf GT_TA -9.4 GT_TC inf GT_TG inf GT_TT inf TA_AA -6.3 TA_AC -5.9 TA_AG -4.7 TA_AT -14.2 TA_CA inf TA_CC inf TA_CG inf TA_CT -6.3 TA_GA inf TA_GC inf TA_GG inf TA_GT -10.5 TA_TA inf TA_TC inf TA_TG inf TA_TT -8.9 TC_AA -7.0 TC_AC -1.3 TC_AG -16.1 TC_AT -6.3 TC_CA inf TC_CC inf TC_CG -13.5 TC_CT inf TC_GA inf TC_GC inf TC_GG -15.9 TC_GT inf TC_TA inf TC_TC inf TC_TG -21.2 TC_TT inf TG_AA -5.8 TG_AC -16.1 TG_AG -2.7 TG_AT -10.5 TG_CA inf TG_CC -8.0 TG_CG inf TG_CT inf TG_GA inf TG_GC -9.0 TG_GG inf TG_GT inf TG_TA inf TG_TC -16.9 TG_TG inf TG_TT inf TT_AA -13.5 TT_AC -1.2 TT_AG -9.8 TT_AT -8.9 TT_CA -1.7 TT_CC inf TT_CG inf TT_CT inf TT_GA -8.7 TT_GC inf TT_GG inf TT_GT inf TT_TA -6.5 TT_TC inf TT_TG inf TT_TT inf primer3-2.3.6/test/primer3_config/interpretations/tstack2_i.dh0000664000077300007730000000466612231156245023411 0ustar ioanaioanaAA_AA 0 AA_AC 0 AA_AG 0 AA_AT -2500 AA_CA 0 AA_CC 0 AA_CG 0 AA_CT -2700 AA_GA 0 AA_GC 0 AA_GG 0 AA_GT -2400 AA_TA -3100 AA_TC -1600 AA_TG -1900 AA_TT -5000 AC_AA 0 AC_AC 0 AC_AG -8000 AC_AT 0 AC_CA 0 AC_CC 0 AC_CG -3200 AC_CT 0 AC_GA 0 AC_GC 0 AC_GG -4600 AC_GT 0 AC_TA -1800 AC_TC -100 AC_TG -6000 AC_TT -900 AG_AA 0 AG_AC -4300 AG_AG 0 AG_AT 0 AG_CA 0 AG_CC -2700 AG_CG 0 AG_CT 0 AG_GA 0 AG_GC -6000 AG_GG 0 AG_GT 0 AG_TA -2500 AG_TC -6000 AG_TG -1100 AG_TT -3200 AT_AA -3100 AT_AC 0 AT_AG 0 AT_AT 0 AT_CA -1800 AT_CC 0 AT_CG 0 AT_CT 0 AT_GA -2500 AT_GC 0 AT_GG 0 AT_GT 0 AT_TA -5000 AT_TC -2300 AT_TG -3500 AT_TT -2400 CA_AA 0 CA_AC 0 CA_AG 0 CA_AT -2300 CA_CA 0 CA_CC 0 CA_CG 0 CA_CT -700 CA_GA -4300 CA_GC -2600 CA_GG -3900 CA_GT -6000 CA_TA 0 CA_TC 0 CA_TG 0 CA_TT -700 CC_AA 0 CC_AC 0 CC_AG -5000 CC_AT 0 CC_CA 0 CC_CC 0 CC_CG -3900 CC_CT 0 CC_GA -2700 CC_GC -2100 CC_GG -7000 CC_GT -3200 CC_TA 0 CC_TC 0 CC_TG -3000 CC_TT 0 CG_AA 0 CG_AC -2600 CG_AG 0 CG_AT 0 CG_CA 0 CG_CC -2100 CG_CG 0 CG_CT 0 CG_GA -6000 CG_GC -7000 CG_GG -3800 CG_GT -3800 CG_TA 0 CG_TC -3900 CG_TG 0 CG_TT 0 CT_AA -1600 CT_AC 0 CT_AG 0 CT_AT 0 CT_CA -100 CT_CC 0 CT_CG 0 CT_CT 0 CT_GA -6000 CT_GC -3900 CT_GG -6600 CT_GT -6100 CT_TA -2300 CT_TC 0 CT_TG 0 CT_TT 0 GA_AA 0 GA_AC 0 GA_AG 0 GA_AT -2000 GA_CA -8000 GA_CC -5000 GA_CG -4300 GA_CT -6000 GA_GA 0 GA_GC 0 GA_GG 0 GA_GT -1100 GA_TA 0 GA_TC 0 GA_TG 0 GA_TT -3600 GC_AA 0 GC_AC 0 GC_AG -4300 GC_AT 0 GC_CA -3200 GC_CC -3900 GC_CG -7000 GC_CT -4900 GC_GA 0 GC_GC 0 GC_GG -700 GC_GT 0 GC_TA 0 GC_TC 0 GC_TG -5900 GC_TT 0 GG_AA 0 GG_AC -3900 GG_AG 0 GG_AT 0 GG_CA -4600 GG_CC -7000 GG_CG -700 GG_CT -5700 GG_GA 0 GG_GC -3800 GG_GG 0 GG_GT 0 GG_TA 0 GG_TC -6600 GG_TG 0 GG_TT 0 GT_AA -1900 GT_AC 0 GT_AG 0 GT_AT 0 GT_CA -6000 GT_CC -3000 GT_CG -5900 GT_CT -7400 GT_GA -1100 GT_GC 0 GT_GG 0 GT_GT 0 GT_TA -3500 GT_TC 0 GT_TG 0 GT_TT 0 TA_AA -2500 TA_AC -2300 TA_AG -2000 TA_AT -5000 TA_CA 0 TA_CC 0 TA_CG 0 TA_CT -2500 TA_GA 0 TA_GC 0 TA_GG 0 TA_GT -3900 TA_TA 0 TA_TC 0 TA_TG 0 TA_TT -3200 TC_AA -2700 TC_AC -700 TC_AG -6000 TC_AT -2500 TC_CA 0 TC_CC 0 TC_CG -4900 TC_CT 0 TC_GA 0 TC_GC 0 TC_GG -5700 TC_GT 0 TC_TA 0 TC_TC 0 TC_TG -7400 TC_TT 0 TG_AA -2400 TG_AC -6000 TG_AG -1100 TG_AT -3900 TG_CA 0 TG_CC -3200 TG_CG 0 TG_CT 0 TG_GA 0 TG_GC -3800 TG_GG 0 TG_GT 0 TG_TA 0 TG_TC -6100 TG_TG 0 TG_TT 0 TT_AA -5000 TT_AC -700 TT_AG -3600 TT_AT -3200 TT_CA -900 TT_CC 0 TT_CG 0 TT_CT 0 TT_GA -3200 TT_GC 0 TT_GG 0 TT_GT 0 TT_TA -2400 TT_TC 0 TT_TG 0 TT_TT 0 primer3-2.3.6/test/primer3_config/interpretations/tstack_i.dh0000664000077300007730000000412712231156245023317 0ustar ioanaioanaAA_AA 0 AA_AC 0 AA_AG 0 AA_AT 25 AA_CA 0 AA_CC 0 AA_CG 0 AA_CT 27 AA_GA 0 AA_GC 0 AA_GG 0 AA_GT 24 AA_TA 31 AA_TC 16 AA_TG 19 AA_TT 0 AC_AA 0 AC_AC 0 AC_AG 80 AC_AT 0 AC_CA 0 AC_CC 0 AC_CG 32 AC_CT 0 AC_GA 0 AC_GC 0 AC_GG 46 AC_GT 0 AC_TA 18 AC_TC 1 AC_TG 0 AC_TT 9 AG_AA 0 AG_AC 43 AG_AG 0 AG_AT 0 AG_CA 0 AG_CC 27 AG_CG 0 AG_CT 0 AG_GA 0 AG_GC 60 AG_GG 0 AG_GT 0 AG_TA 25 AG_TC 0 AG_TG 11 AG_TT 32 AT_AA 31 AT_AC 0 AT_AG 0 AT_AT 0 AT_CA 18 AT_CC 0 AT_CG 0 AT_CT 0 AT_GA 25 AT_GC 0 AT_GG 0 AT_GT 0 AT_TA 0 AT_TC 23 AT_TG 35 AT_TT 24 CA_AA 0 CA_AC 0 CA_AG 0 CA_AT 23 CA_CA 0 CA_CC 0 CA_CG 0 CA_CT 7 CA_GA 43 CA_GC 26 CA_GG 39 CA_GT 0 CA_TA 0 CA_TC 0 CA_TG 0 CA_TT 7 CC_AA 0 CC_AC 0 CC_AG 50 CC_AT 0 CC_CA 0 CC_CC 0 CC_CG 39 CC_CT 0 CC_GA 27 CC_GC 21 CC_GG 0 CC_GT 32 CC_TA 0 CC_TC 0 CC_TG 30 CC_TT 0 CG_AA 0 CG_AC 26 CG_AG 0 CG_AT 0 CG_CA 0 CG_CC 21 CG_CG 0 CG_CT 0 CG_GA 60 CG_GC 0 CG_GG 38 CG_GT 38 CG_TA 0 CG_TC 39 CG_TG 0 CG_TT 0 CT_AA 16 CT_AC 0 CT_AG 0 CT_AT 0 CT_CA 1 CT_CC 0 CT_CG 0 CT_CT 0 CT_GA 0 CT_GC 39 CT_GG 66 CT_GT 61 CT_TA 23 CT_TC 0 CT_TG 0 CT_TT 0 GA_AA 0 GA_AC 0 GA_AG 0 GA_AT 20 GA_CA 80 GA_CC 50 GA_CG 43 GA_CT 0 GA_GA 0 GA_GC 0 GA_GG 0 GA_GT 11 GA_TA 0 GA_TC 0 GA_TG 0 GA_TT 36 GC_AA 0 GC_AC 0 GC_AG 43 GC_AT 0 GC_CA 32 GC_CC 39 GC_CG 0 GC_CT 49 GC_GA 0 GC_GC 0 GC_GG 7 GC_GT 0 GC_TA 0 GC_TC 0 GC_TG 59 GC_TT 0 GG_AA 0 GG_AC 39 GG_AG 0 GG_AT 0 GG_CA 46 GG_CC 0 GG_CG 7 GG_CT 57 GG_GA 0 GG_GC 38 GG_GG 0 GG_GT 0 GG_TA 0 GG_TC 66 GG_TG 0 GG_TT 0 GT_AA 19 GT_AC 0 GT_AG 0 GT_AT 0 GT_CA 0 GT_CC 30 GT_CG 59 GT_CT 74 GT_GA 11 GT_GC 0 GT_GG 0 GT_GT 0 GT_TA 35 GT_TC 0 GT_TG 0 GT_TT 0 TA_AA 25 TA_AC 23 TA_AG 20 TA_AT 72 TA_CA 0 TA_CC 0 TA_CG 0 TA_CT 25 TA_GA 0 TA_GC 0 TA_GG 0 TA_GT 39 TA_TA 0 TA_TC 0 TA_TG 0 TA_TT 32 TC_AA 27 TC_AC 7 TC_AG 0 TC_AT 25 TC_CA 0 TC_CC 0 TC_CG 49 TC_CT 0 TC_GA 0 TC_GC 0 TC_GG 57 TC_GT 0 TC_TA 0 TC_TC 0 TC_TG 74 TC_TT 0 TG_AA 24 TG_AC 0 TG_AG 11 TG_AT 39 TG_CA 0 TG_CC 32 TG_CG 0 TG_CT 0 TG_GA 0 TG_GC 38 TG_GG 0 TG_GT 0 TG_TA 0 TG_TC 61 TG_TG 0 TG_TT 0 TT_AA 0 TT_AC 7 TT_AG 36 TT_AT 32 TT_CA 9 TT_CC 0 TT_CG 0 TT_CT 0 TT_GA 32 TT_GC 0 TT_GG 0 TT_GT 0 TT_TA 24 TT_TC 0 TT_TG 0 TT_TT 0 primer3-2.3.6/test/primer3_config/interpretations/dangle_i.ds0000664000077300007730000000177112231156245023275 0ustar ioanaioana/* Entropy of dangling end */ /* 3' dangling */ AA_A 0 AC_A 0 AG_A 0 AT_A 0 AA_C 0 AC_C 0 AG_C 0 AT_C 0 AA_G 0 AC_G 0 AG_G 0 AT_G 0 AA_T 11 AC_T -142 AG_T 131 AT_T 126 CA_A 0 CC_A 0 CG_A 0 CT_A 0 CA_C 0 CC_C 0 CG_C 0 CT_C 0 CA_G 165 CC_G 74 CG_G 104 CT_G 150 CA_T 0 CC_T 0 CG_T 0 CT_T 0 GA_A 0 GC_A 0 GG_A 0 GT_A 0 GA_C 39 GC_C 1 GG_C 112 GT_C 131 GA_G 0 GC_G 0 GG_G 0 GT_G 0 GA_T 0 GC_T 0 GG_T 0 GT_T 0 TA_A 8 TC_A -149 TG_A 36 TT_A -104 TA_C 0 TC_C 0 TG_C 0 TT_C 0 TA_G 0 TC_G 0 TG_G 0 TT_G 0 TA_T 0 TC_T 0 TG_T 0 TT_T 0 /* 5' dangling */ A_AA 0 A_AC 0 A_AG 0 A_AT 0 A_CA 0 A_CC 0 A_CG 0 A_CT 0 A_GA 0 A_GC 0 A_GG 0 A_GT 0 A_TA 76 A_TC 130 A_TG 150 A_TT 5 C_AA 0 C_AC 0 C_AG 0 C_AT 0 C_CA 0 C_CC 0 C_CG 0 C_CT 0 C_GA 100 C_GC 119 C_GG 109 C_GT 138 C_TA 0 C_TC 0 C_TG 0 C_TT 0 G_AA 0 G_AC 0 G_AG 0 G_AT 0 G_CA 171 G_CC 126 G_CG 140 G_CT 109 G_GA 0 G_GC 0 G_GG 0 G_GT 0 G_TA 0 G_TC 0 G_TG 0 G_TT 0 T_AA -23 T_AC -33 T_AG 16 T_AT 200 T_CA 0 T_CC 0 T_CG 0 T_CT 0 T_GA 0 T_GC 0 T_GG 0 T_GT 0 T_TA 0 T_TC 0 T_TG 0 T_TT 0 primer3-2.3.6/test/primer3_config/interpretations/loops_i.dh0000664000077300007730000000350012231156245023154 0ustar ioanaioanaDESTABILIZING ENTHALPIES BY SIZE OF LOOP (INTERPOLATE WHERE NEEDED) hp3 ave calc no tmm;hp4 ave calc with tmm; ave all bulges SIZE INTERNAL BULGE HAIRPIN ------------------------------------------------------- 1 . 0.0 . 2 0.0 0.0 . 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 primer3-2.3.6/test/primer3_config/interpretations/tetraloop_i.ds0000664000077300007730000000155112231156245024050 0ustar ioanaioana/* VIIDE */ AAAAAT 6.5 AAAACT -16.1 AAACAT -16.1 ACTTGT -41.9 AGAAAT -16.1 AGAGAT -16.1 AGATAT -16.1 AGCAAT -16.1 AGCGAT -16.1 AGCTTT -16.1 AGGAAT -16.1 AGGGAT -16.1 AGGGGT -6.4 AGTAAT -16.1 AGTGAT -16.1 AGTTCT -16.1 ATTCGT -16.1 ATTTGT -16.1 ATTTTT -16.1 CAAAAG 12.9 CAAACG 0 CAACAG 0 CAACCG 0 CCTTGG -25.7 CGAAAG 0 CGAGAG 0 CGATAG 0 CGCAAG 0 CGCGAG 0 CGCTTG 0 CGGAAG 0 CGGGAG 0 CGGGGG 9.7 CGTAAG 0 CGTGAG 0 CGTTCG 0 CTTCGG 0 CTTTGG 0 CTTTTG 0 GAAAAC 32.3 GAAACC 0 GAACAC 0 GCTTGC -25.7 GGAAAC 0 GGAGAC 0 GGATAC 0 GGCAAC 0 GGCGAC 0 GGCTTC 0 GGGAAC 0 GGGGAC 0 GGGGGC 9.7 GGTAAC 0 GGTGAC 0 GGTTCC 0 GTTCGC 0 GTTTGC 0 GTTTTC 0 TAAAAA -3.2 TAAACA -16.1 TAACAA -16.1 TCTTGA -41.9 TGAAAA -16.1 TGAGAA -16.1 TGATAA -16.1 TGCAAA -16.1 TGCGAA -16.1 TGCTTA -16.1 TGGAAA -16.1 TGGGAA -16.1 TGGGGA -6.4 TGTAAA -16.1 TGTGAA -16.1 TGTTCA -16.1 TTTCGA -16.1 TTTTGA -16.1 TTTTTA -16.1 primer3-2.3.6/test/primer3_config/interpretations/stack_i.ds0000664000077300007730000000504512231156245023146 0ustar ioanaioanaAA_AA inf AA_AC inf AA_AG inf AA_AT inf AA_CA inf AA_CC inf AA_CG inf AA_CT inf AA_GA inf AA_GC inf AA_GG inf AA_GT inf AA_TA inf AA_TC inf AA_TG inf AA_TT -22.2 AC_AA inf AC_AC inf AC_AG inf AC_AT inf AC_CA inf AC_CC inf AC_CG inf AC_CT inf AC_GA inf AC_GC inf AC_GG inf AC_GT inf AC_TA inf AC_TC inf AC_TG -22.4 AC_TT inf AG_AA inf AG_AC inf AG_AG inf AG_AT inf AG_CA inf AG_CC inf AG_CG inf AG_CT inf AG_GA inf AG_GC -13.2 AG_GG inf AG_GT inf AG_TA inf AG_TC -21.0 AG_TG inf AG_TT inf AT_AA inf AT_AC inf AT_AG inf AT_AT inf AT_CA inf AT_CC inf AT_CG inf AT_CT inf AT_GA inf AT_GC inf AT_GG inf AT_GT inf AT_TA -20.4 AT_TC inf AT_TG inf AT_TT inf CA_AA inf CA_AC inf CA_AG inf CA_AT inf CA_CA inf CA_CC inf CA_CG inf CA_CT inf CA_GA inf CA_GC inf CA_GG inf CA_GT -22.7 CA_TA inf CA_TC inf CA_TG inf CA_TT inf CC_AA inf CC_AC inf CC_AG inf CC_AT inf CC_CA inf CC_CC inf CC_CG inf CC_CT inf CC_GA inf CC_GC inf CC_GG -19.9 CC_GT inf CC_TA inf CC_TC inf CC_TG inf CC_TT inf CG_AA inf CG_AC inf CG_AG inf CG_AT inf CG_CA inf CG_CC inf CG_CG inf CG_CT inf CG_GA inf CG_GC -27.2 CG_GG inf CG_GT inf CG_TA inf CG_TC inf CG_TG inf CG_TT inf CT_AA inf CT_AC inf CT_AG inf CT_AT inf CT_CA inf CT_CC inf CT_CG inf CT_CT inf CT_GA -21.0 CT_GC inf CT_GG inf CT_GT -15.8 CT_TA inf CT_TC inf CT_TG inf CT_TT inf GA_AA inf GA_AC inf GA_AG inf GA_AT inf GA_CA inf GA_CC inf GA_CG inf GA_CT -22.2 GA_GA inf GA_GC inf GA_GG inf GA_GT inf GA_TA inf GA_TC inf GA_TG inf GA_TT inf GC_AA inf GC_AC inf GC_AG inf GC_AT inf GC_CA inf GC_CC inf GC_CG -24.4 GC_CT inf GC_GA inf GC_GC inf GC_GG inf GC_GT inf GC_TA inf GC_TC inf GC_TG inf GC_TT inf GG_AA inf GG_AC inf GG_AG inf GG_AT inf GG_CA inf GG_CC -19.9 GG_CG -15.8 GG_CT inf GG_GA inf GG_GC inf GG_GG inf GG_GT inf GG_TA inf GG_TC inf GG_TG inf GG_TT inf GT_AA inf GT_AC inf GT_AG inf GT_AT inf GT_CA -22.4 GT_CC inf GT_CG inf GT_CT inf GT_GA inf GT_GC inf GT_GG inf GT_GT inf GT_TA inf GT_TC inf GT_TG inf GT_TT inf TA_AA inf TA_AC inf TA_AG inf TA_AT -21.3 TA_CA inf TA_CC inf TA_CG inf TA_CT inf TA_GA inf TA_GC inf TA_GG inf TA_GT inf TA_TA inf TA_TC inf TA_TG inf TA_TT inf TC_AA inf TC_AC inf TC_AG -22.2 TC_AT inf TC_CA inf TC_CC inf TC_CG inf TC_CT inf TC_GA inf TC_GC inf TC_GG inf TC_GT inf TC_TA inf TC_TC inf TC_TG inf TC_TT inf TG_AA inf TG_AC -22.7 TG_AG inf TG_AT inf TG_CA inf TG_CC inf TG_CG inf TG_CT inf TG_GA inf TG_GC inf TG_GG inf TG_GT inf TG_TA inf TG_TC inf TG_TG inf TG_TT inf TT_AA -22.2 TT_AC inf TT_AG inf TT_AT inf TT_CA inf TT_CC inf TT_CG inf TT_CT inf TT_GA -9 TT_GC inf TT_GG inf TT_GT inf TT_TA inf TT_TC inf TT_TG inf TT_TT inf primer3-2.3.6/test/primer3_config/interpretations/loops_i.ds0000664000077300007730000000127312231156245023174 0ustar ioanaioanaSIZE INTERNAL BULGE HAIRPIN 1 . -128.9 . 2 . -93.5 . 3 -103.1 -99.9 -112.8 4 -116 -103.1 -112.8 5 -128.9 -106.4 -106.4 6 -141.8 -112.8 -128.9 7 -148.3 -119.2 -135.4 8 -154.7 -125.7 -138.6 9 -157.9 -132.1 -145 10 -157.9 -138.6 -148.3 11 -162.6 -143.2 -152.9 12 -167.6 -145 -161.2 13 -171.5 -148.9 -165 14 -174.1 -154.7 -164.4 15 -177.4 -158.1 -167.7 16 -180.5 -161.2 -170.8 17 -183.4 -164.1 -173.8 18 -187 -167.6 -177.3 19 -189.6 -170.2 -179.9 20 -190.2 -170.8 -183.7 21 -192.5 -173.2 -186.1 22 -194.8 -175.5 -188.4 23 -197 -177.6 -190.5 24 -199 -179.7 -192.6 25 -203.1 -180.5 -196.6 26 -205 -182.4 -198.5 27 -206.8 -184.2 -200.4 28 -208.6 -186 -202.1 29 -210.3 -187.7 -203.8 30 -212.8 -190.2 -203.1 primer3-2.3.6/test/primer3_config/stack.ds0000664000077300007730000000204012231156245017374 0ustar ioanaioanainf inf inf inf inf inf inf inf inf inf inf inf inf inf inf -22.2 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -22.4 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -21.0 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -20.4 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -22.7 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -19.9 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -27.2 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -21.0 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -22.2 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -24.4 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -19.9 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -22.4 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -21.3 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -22.2 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -22.7 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -22.2 inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf primer3-2.3.6/test/primer3_config/stackmm.dh0000664000077300007730000000217112231156245017720 0ustar ioanaioanainf inf inf 4700 inf inf inf 7600 inf inf inf 3000 1200 2300 -600 inf inf inf -2900 inf inf inf -700 inf inf inf 500 inf 5300 -10 inf 700 inf -900 inf inf inf 600 inf inf inf -4000 inf inf -700 inf -3100 1000 1200 inf inf inf 5300 inf inf inf -700 inf inf inf inf -1200 -2500 -2700 inf inf inf 3400 inf inf inf 6100 -900 1900 -700 inf inf inf inf 1000 inf inf 5200 inf inf inf 3600 inf 600 -1500 inf -800 inf inf 5200 inf inf 1900 inf inf inf -1500 inf inf -4000 inf -4900 -4100 inf -1500 inf inf 2300 inf inf inf -10 inf inf inf inf -1500 -2800 -5000 -1200 inf inf inf inf inf inf 700 -2900 5200 -600 inf inf inf inf 1600 inf inf inf -1300 inf inf -600 inf -700 3600 inf 2300 inf inf -6000 inf inf inf -4400 inf inf -700 inf inf 500 inf -6000 3300 inf -4900 inf inf inf -2800 inf 5800 -600 inf inf inf inf 5200 -4400 -2200 -3100 inf inf inf -2500 inf 4100 inf 4700 3400 700 inf inf inf inf 1200 inf inf inf -100 inf inf inf 200 7600 6100 inf 1200 inf inf 2300 inf inf inf 3300 inf inf inf -2200 inf 3000 inf 1600 -100 inf -800 inf inf inf -4100 inf -1400 inf -5000 inf inf inf 1000 -1300 200 700 inf inf inf 1000 inf 5800 inf -2700 inf inf inf primer3-2.3.6/test/primer3_config/tstack2.dh0000664000077300007730000000166612231156245017644 0ustar ioanaioana0 0 0 -2500 0 0 0 -2700 0 0 0 -2400 -3100 -1600 -1900 -5000 0 0 -8000 0 0 0 -3200 0 0 0 -4600 0 -1800 -100 -6000 -900 0 -4300 0 0 0 -2700 0 0 0 -6000 0 0 -2500 -6000 -1100 -3200 -3100 0 0 0 -1800 0 0 0 -2500 0 0 0 -5000 -2300 -3500 -2400 0 0 0 -2300 0 0 0 -700 -4300 -2600 -3900 -6000 0 0 0 -700 0 0 -5000 0 0 0 -3900 0 -2700 -2100 -7000 -3200 0 0 -3000 0 0 -2600 0 0 0 -2100 0 0 -6000 -7000 -3800 -3800 0 -3900 0 0 -1600 0 0 0 -100 0 0 0 -6000 -3900 -6600 -6100 -2300 0 0 0 0 0 0 -2000 -8000 -5000 -4300 -6000 0 0 0 -1100 0 0 0 -3600 0 0 -4300 0 -3200 -3900 -7000 -4900 0 0 -700 0 0 0 -5900 0 0 -3900 0 0 -4600 -7000 -700 -5700 0 -3800 0 0 0 -6600 0 0 -1900 0 0 0 -6000 -3000 -5900 -7400 -1100 0 0 0 -3500 0 0 0 -2500 -2300 -2000 -5000 0 0 0 -2500 0 0 0 -3900 0 0 0 -3200 -2700 -700 -6000 -2500 0 0 -4900 0 0 0 -5700 0 0 0 -7400 0 -2400 -6000 -1100 -3900 0 -3200 0 0 0 -3800 0 0 0 -6100 0 0 -5000 -700 -3600 -3200 -900 0 0 0 -3200 0 0 0 -2400 0 0 0 primer3-2.3.6/test/primer3_config/loops.ds0000664000077300007730000000126412231156245017432 0ustar ioanaioana1 -1.0 -12.89 -1.0 2 -1.0 -9.35 -1.0 3 -10.31 -9.99 -11.28 4 -11.6 -10.31 -11.28 5 -12.89 -10.64 -10.64 6 -14.18 -11.28 -12.89 7 -14.83 -11.92 -13.54 8 -15.47 -12.57 -13.86 9 -15.79 -13.21 -14.5 10 -15.79 -13.86 -14.83 11 -16.26 -14.32 -15.29 12 -16.76 -14.5 -16.12 13 -17.15 -14.89 -16.5 14 -17.41 -15.47 -16.44 15 -17.74 -15.81 -16.77 16 -18.05 -16.12 -17.08 17 -18.34 -16.41 -17.38 18 -18.7 -16.76 -17.73 19 -18.96 -17.02 -17.99 20 -19.02 -17.08 -18.37 21 -19.25 -17.32 -18.61 22 -19.48 -17.55 -18.84 23 -19.7 -17.76 -19.05 24 -19.9 -17.97 -19.26 25 -20.31 -18.05 -19.66 26 -20.5 -18.24 -19.85 27 -20.68 -18.42 -20.04 28 -20.86 -18.6 -20.21 29 -21.03 -18.77 -20.38 30 -21.28 -19.02 -20.31 primer3-2.3.6/test/primer3_config/triloop.dh0000664000077300007730000000030012231156245017741 0ustar ioanaioanaAGAAT -1500 AGCAT -1500 AGGAT -1500 AGTAT -1500 CGAAG -2000 CGCAG -2000 CGGAG -2000 CGTAG -2000 GGAAC -2000 GGCAC -2000 GGGAC -2000 GGTAC -2000 TGAAA -1500 TGCAA -1500 TGGAA -1500 TGTAA -1500 primer3-2.3.6/test/primer3_config/stack.dh0000664000077300007730000000204112231156245017362 0ustar ioanaioanainf inf inf inf inf inf inf inf inf inf inf inf inf inf inf -7900 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -8400 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -7800 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -7200 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -8500 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -8000 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -10600 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -7800 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -8200 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -9800 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -8000 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -8400 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -7200 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -8200 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -8500 inf inf inf inf inf inf inf inf inf inf inf inf inf inf -7900 inf inf inf inf inf inf inf inf inf inf inf inf inf inf inf primer3-2.3.6/test/primer3_config/tstack_tm_inf.ds0000664000077300007730000000220012231156245021112 0ustar ioanaioanainf inf inf -6.3 inf inf inf -7.0 inf inf inf -5.8 -7.8 -4.0 -4.4 inf inf inf -22.5 inf inf inf -7.1 inf inf inf -11.4 inf -3.8 -0.5 inf -1.7 inf -10.7 inf inf inf -6.0 inf inf inf -15.5 inf inf -5.9 inf -2.1 -8.7 -7.8 inf inf inf -3.8 inf inf inf -5.9 inf inf inf inf -6.3 -9.4 -6.5 inf inf inf -5.9 inf inf inf -1.3 -10.7 -5.9 -9.6 inf inf inf inf -1.2 inf inf -13.8 inf inf inf -10.6 inf -6.0 -5.1 inf -8.0 inf inf -7.8 inf inf -5.9 inf inf inf -5.1 inf inf -15.5 inf -9.5 -9.0 inf -10.6 inf inf -4.0 inf inf inf -0.5 inf inf inf inf -10.6 -18.7 -16.9 -6.3 inf inf inf inf inf inf -4.7 -22.5 -13.8 -11.1 inf inf inf inf -2.7 inf inf inf -9.8 inf inf -11.1 inf -7.1 -10.6 inf -13.5 inf inf -19.2 inf inf inf -16.1 inf inf -9.6 inf inf -11.4 inf -19.2 -15.9 inf -9.5 inf inf inf -18.7 inf inf -4.4 inf inf inf inf -7.8 -16.1 -21.2 -2.1 inf inf inf -9.4 inf inf inf -6.3 -5.9 -4.7 inf inf inf inf -6.3 inf inf inf -10.5 inf inf inf -8.9 -7.0 -1.3 inf -6.3 inf inf -13.5 inf inf inf -15.9 inf inf inf -21.2 inf -5.8 inf -2.7 -10.5 inf -8.0 inf inf inf -9.0 inf inf inf -16.9 inf inf inf -1.2 -9.8 -8.9 -1.7 inf inf inf -8.7 inf inf inf -6.5 inf inf inf primer3-2.3.6/test/primer3_config/stackmm.ds0000664000077300007730000000211712231156245017733 0ustar ioanaioanainf inf inf 12.9 inf inf inf 20.2 inf inf inf 7.4 1.7 4.6 -2.3 inf inf inf -9.8 inf inf inf -3.8 inf inf inf 3.2 inf 14.6 -4.4 inf 0.2 inf -4.2 inf inf inf 0.6 inf inf inf -13.2 inf inf -2.3 inf -9.5 0.9 1.7 inf inf inf 14.6 inf inf inf -2.3 inf inf inf inf -6.2 -8.3 -10.8 inf inf inf 8.0 inf inf inf 16.4 -4.2 3.7 -2.3 inf inf inf inf 0.7 inf inf 14.2 inf inf inf 8.9 inf -0.6 -7.2 inf -4.5 inf inf 13.5 inf inf 3.7 inf inf inf -7.2 inf inf -13.2 inf -15.3 -11.7 inf -6.1 inf inf 4.6 inf inf inf -4.4 inf inf inf inf -6.1 -8.0 -15.8 -6.2 inf inf inf inf inf inf 0.7 -9.8 14.2 -1.0 inf inf inf inf 3.6 inf inf inf -5.3 inf inf -1.0 inf -3.8 8.9 inf 5.4 inf inf -15.8 inf inf inf -12.3 inf inf -2.3 inf inf 3.2 inf -15.8 10.4 inf -15.3 inf inf inf 80 inf 16.3 23 inf inf inf inf 13.5 -12.3 -8.4 -9.5 inf inf inf -8.3 inf 9.5 inf 12.9 8.0 0.7 inf inf inf inf 0.7 inf inf inf -1.7 inf inf inf -1.5 20.2 16.4 inf 0.7 inf inf 5.4 inf inf inf 10.4 inf inf inf -8.4 inf 7.4 inf 3.6 -1.7 inf -4.5 inf inf inf -11.7 inf -6.2 inf -15.8 inf inf inf -7 -5.3 -1.5 0.2 inf inf inf 0.9 inf 16.3 inf -10.8 inf inf inf primer3-2.3.6/test/primer_task_th_input0000664000077300007730000000407412231156245017217 0ustar ioanaioanaPRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 P3_FILE_FLAG=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_TASK=pick_pcr_primers PRIMER_DNTP_CONC=0.0 SEQUENCE_ID=pcr_primers SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 = SEQUENCE_ID=pcr_primers_and_io PRIMER_TASK=pick_pcr_primers_and_hyb_PROBE SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG = SEQUENCE_ID=left_only PRIMER_TASK=pick_left_only SEQUENCE_TARGET=100,20 SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC = SEQUENCE_ID=right_only PRIMER_TASK=PICK_right_only SEQUENCE_TARGET=90,10 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC = SEQUENCE_ID=hyb_probe_only PRIMER_TASK=pick_HYB_probe_ONly SEQUENCE_INTERNAL_EXCLUDED_REGION=90,20 SEQUENCE_TEMPLATE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC = SEQUENCE_ID=left_only PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG = SEQUENCE_ID=right_only PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG = SEQUENCE_ID=hyb_probe_only PRIMER_INTERNAL_MIN_SIZE=20 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG = primer3-2.3.6/test/primer_overlap_junction_input0000664000077300007730000000731112231156245021140 0ustar ioanaioanaSEQUENCE_ID=Details of SEQUENCE_OVERLAP_JUNCTION_LIST and PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION TEST 01 -- OVERLAP P3_COMMENT=The 50th nt is uppercase PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=40-100 PRIMER_MIN_GC=0 PRIMER_MIN_TM=0 PRIMER_PICK_ANYWAY=0 SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION=2 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=2 SEQUENCE_OVERLAP_JUNCTION_LIST=50 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_SIZE=16 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAga = SEQUENCE_ID=Details of SEQUENCE_OVERLAP_JUNCTION_LIST and PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION TEST 02 -- OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg = SEQUENCE_ID=Details of SEQUENCE_OVERLAP_JUNCTION_LIST and PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION TEST 03 -- NO OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccA = SEQUENCE_ID=Details of SEQUENCE_OVERLAP_JUNCTION_LIST and PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION TEST 08 -- OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=ttgttcattctgtttcTgg SEQUENCE_PRIMER_REVCOMP=catcacacccttttacacatt = SEQUENCE_ID=Details of SEQUENCE_OVERLAP_JUNCTION_LIST and PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION TEST 09 -- NO OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=ttgttcattctgtttcTg SEQUENCE_PRIMER_REVCOMP=catcacacccttttacacatt = SEQUENCE_ID=Test for 5' overlap on reverse primer -- OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=3 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=tccAgaaacagaatgaacaatag = SEQUENCE_ID=Test for 5' overlap on reverse primer -- NO OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=ccAgaaacagaatgaacaatag = SEQUENCE_ID=Test for 5' overlap on reverse primer -- NO OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=cAgaaacagaatgaacaatag = SEQUENCE_ID=Test for 5' overlap on forward primer -- OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=tcTggattgtgtttttttc SEQUENCE_PRIMER_REVCOMP=catcacacccttttacacatt = SEQUENCE_ID=Test for 5' overlap on forward primer -- NO OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=cTggattgtgtttttttc SEQUENCE_PRIMER_REVCOMP=catcacacccttttacacatt =primer3-2.3.6/test/primer1_th_output0000664000077300007730000012113112231156245016451 0ustar ioanaioanaPRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_PARAMETERS_PATH=../primer3_config/ PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 P3_COMMENT=The next line to reproduce behavior with -default_version=1 PRIMER_DNTP_CONC=0.0 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 P3_FILE_FLAG=1 SEQUENCE_ID=targets_test SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=22,122 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=SP01-02.txt SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.564009 PRIMER_LEFT_0_PENALTY=2.796318 PRIMER_RIGHT_0_PENALTY=0.767691 PRIMER_LEFT_0_SEQUENCE=GCTGTTTCACACTTACGGAA PRIMER_RIGHT_0_SEQUENCE=TGTTGGAGAAAAGCCACTGG PRIMER_LEFT_0=8,20 PRIMER_RIGHT_0=245,20 PRIMER_LEFT_0_TM=57.204 PRIMER_RIGHT_0_TM=59.232 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=35.22 PRIMER_LEFT_0_END_STABILITY=4.3000 PRIMER_RIGHT_0_END_STABILITY=4.0000 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.32 PRIMER_PAIR_0_PRODUCT_SIZE=238 PRIMER_PAIR_1_PENALTY=3.818870 PRIMER_LEFT_1_PENALTY=3.051179 PRIMER_RIGHT_1_PENALTY=0.767691 PRIMER_LEFT_1_SEQUENCE=GCTGTTTCACACTTACGGAAA PRIMER_RIGHT_1_SEQUENCE=TGTTGGAGAAAAGCCACTGG PRIMER_LEFT_1=8,21 PRIMER_RIGHT_1=245,20 PRIMER_LEFT_1_TM=57.949 PRIMER_RIGHT_1_TM=59.232 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=35.22 PRIMER_LEFT_1_END_STABILITY=3.1300 PRIMER_RIGHT_1_END_STABILITY=4.0000 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.32 PRIMER_PAIR_1_PRODUCT_SIZE=238 PRIMER_PAIR_2_PENALTY=3.853878 PRIMER_LEFT_2_PENALTY=2.796318 PRIMER_RIGHT_2_PENALTY=1.057560 PRIMER_LEFT_2_SEQUENCE=GCTGTTTCACACTTACGGAA PRIMER_RIGHT_2_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_LEFT_2=8,20 PRIMER_RIGHT_2=248,20 PRIMER_LEFT_2_TM=57.204 PRIMER_RIGHT_2_TM=58.942 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=38.11 PRIMER_LEFT_2_END_STABILITY=4.3000 PRIMER_RIGHT_2_END_STABILITY=5.0100 PRIMER_PAIR_2_COMPL_ANY_TH=4.27 PRIMER_PAIR_2_COMPL_END_TH=4.03 PRIMER_PAIR_2_PRODUCT_SIZE=241 PRIMER_PAIR_3_PENALTY=3.857112 PRIMER_LEFT_3_PENALTY=2.796318 PRIMER_RIGHT_3_PENALTY=1.060794 PRIMER_LEFT_3_SEQUENCE=GCTGTTTCACACTTACGGAA PRIMER_RIGHT_3_SEQUENCE=CCTGTTGGAGAAAAGCCACT PRIMER_LEFT_3=8,20 PRIMER_RIGHT_3=247,20 PRIMER_LEFT_3_TM=57.204 PRIMER_RIGHT_3_TM=58.939 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=35.22 PRIMER_LEFT_3_END_STABILITY=4.3000 PRIMER_RIGHT_3_END_STABILITY=4.0000 PRIMER_PAIR_3_COMPL_ANY_TH=3.04 PRIMER_PAIR_3_COMPL_END_TH=2.28 PRIMER_PAIR_3_PRODUCT_SIZE=240 PRIMER_PAIR_4_PENALTY=4.080246 PRIMER_LEFT_4_PENALTY=3.312555 PRIMER_RIGHT_4_PENALTY=0.767691 PRIMER_LEFT_4_SEQUENCE=GCTGTTTCACACTTACGGAAATG PRIMER_RIGHT_4_SEQUENCE=TGTTGGAGAAAAGCCACTGG PRIMER_LEFT_4=8,23 PRIMER_RIGHT_4=245,20 PRIMER_LEFT_4_TM=59.687 PRIMER_RIGHT_4_TM=59.232 PRIMER_LEFT_4_GC_PERCENT=43.478 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=35.22 PRIMER_LEFT_4_END_STABILITY=2.3200 PRIMER_RIGHT_4_END_STABILITY=4.0000 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.32 PRIMER_PAIR_4_PRODUCT_SIZE=238 = SEQUENCE_ID=SP01-03.txt SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.582080 PRIMER_LEFT_0_PENALTY=0.199248 PRIMER_RIGHT_0_PENALTY=0.382831 PRIMER_LEFT_0_SEQUENCE=TGCAGGTTTAGAAGGGGTGT PRIMER_RIGHT_0_SEQUENCE=AAGCAAATGGACGTGGCATC PRIMER_LEFT_0=66,20 PRIMER_RIGHT_0=165,20 PRIMER_LEFT_0_TM=59.801 PRIMER_RIGHT_0_TM=60.383 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.1600 PRIMER_RIGHT_0_END_STABILITY=3.9100 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=0.582080 PRIMER_LEFT_1_PENALTY=0.199248 PRIMER_RIGHT_1_PENALTY=0.382831 PRIMER_LEFT_1_SEQUENCE=TGCAGGTTTAGAAGGGGTGT PRIMER_RIGHT_1_SEQUENCE=GAAGCAAATGGACGTGGCAT PRIMER_LEFT_1=66,20 PRIMER_RIGHT_1=166,20 PRIMER_LEFT_1_TM=59.801 PRIMER_RIGHT_1_TM=60.383 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=4.1600 PRIMER_RIGHT_1_END_STABILITY=4.4000 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=101 PRIMER_PAIR_2_PENALTY=0.625979 PRIMER_LEFT_2_PENALTY=0.243148 PRIMER_RIGHT_2_PENALTY=0.382831 PRIMER_LEFT_2_SEQUENCE=CCTGCAGGTTTAGAAGGGGT PRIMER_RIGHT_2_SEQUENCE=AAGCAAATGGACGTGGCATC PRIMER_LEFT_2=64,20 PRIMER_RIGHT_2=165,20 PRIMER_LEFT_2_TM=60.243 PRIMER_RIGHT_2_TM=60.383 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY_TH=26.16 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=4.9500 PRIMER_RIGHT_2_END_STABILITY=3.9100 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=102 PRIMER_PAIR_3_PENALTY=0.625979 PRIMER_LEFT_3_PENALTY=0.243148 PRIMER_RIGHT_3_PENALTY=0.382831 PRIMER_LEFT_3_SEQUENCE=CCTGCAGGTTTAGAAGGGGT PRIMER_RIGHT_3_SEQUENCE=GAAGCAAATGGACGTGGCAT PRIMER_LEFT_3=64,20 PRIMER_RIGHT_3=166,20 PRIMER_LEFT_3_TM=60.243 PRIMER_RIGHT_3_TM=60.383 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY_TH=26.16 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=4.9500 PRIMER_RIGHT_3_END_STABILITY=4.4000 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=103 PRIMER_PAIR_4_PENALTY=0.734986 PRIMER_LEFT_4_PENALTY=0.423041 PRIMER_RIGHT_4_PENALTY=0.311945 PRIMER_LEFT_4_SEQUENCE=ATCTGCACCCTGTAAACCCT PRIMER_RIGHT_4_SEQUENCE=GTCACCAAATGCACCCAGAC PRIMER_LEFT_4=3,20 PRIMER_RIGHT_4=103,20 PRIMER_LEFT_4_TM=59.577 PRIMER_RIGHT_4_TM=60.312 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=4.3400 PRIMER_RIGHT_4_END_STABILITY=3.5100 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=101 = SEQUENCE_ID=SP01-06.txt SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=8.875269 PRIMER_LEFT_0_PENALTY=6.659117 PRIMER_RIGHT_0_PENALTY=2.216152 PRIMER_LEFT_0_SEQUENCE=TCGCAATCAAATTTTTTCTATGACCT PRIMER_RIGHT_0_SEQUENCE=GATCGGGAGCGTGCTTTG PRIMER_LEFT_0=37,26 PRIMER_RIGHT_0=136,18 PRIMER_LEFT_0_TM=59.341 PRIMER_RIGHT_0_TM=59.784 PRIMER_LEFT_0_GC_PERCENT=30.769 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=2.7700 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=9.102381 PRIMER_LEFT_1_PENALTY=6.886230 PRIMER_RIGHT_1_PENALTY=2.216152 PRIMER_LEFT_1_SEQUENCE=TCGCAATCAAATTTTTTCTATGACC PRIMER_RIGHT_1_SEQUENCE=GATCGGGAGCGTGCTTTG PRIMER_LEFT_1=37,25 PRIMER_RIGHT_1=136,18 PRIMER_LEFT_1_TM=58.114 PRIMER_RIGHT_1_TM=59.784 PRIMER_LEFT_1_GC_PERCENT=32.000 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=4.0200 PRIMER_RIGHT_1_END_STABILITY=2.7700 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=9.521198 PRIMER_LEFT_2_PENALTY=7.305046 PRIMER_RIGHT_2_PENALTY=2.216152 PRIMER_LEFT_2_SEQUENCE=ATCGCAATCAAATTTTTTCTATGACCT PRIMER_RIGHT_2_SEQUENCE=GATCGGGAGCGTGCTTTG PRIMER_LEFT_2=36,27 PRIMER_RIGHT_2=136,18 PRIMER_LEFT_2_TM=59.695 PRIMER_RIGHT_2_TM=59.784 PRIMER_LEFT_2_GC_PERCENT=29.630 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=3.8500 PRIMER_RIGHT_2_END_STABILITY=2.7700 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=101 PRIMER_PAIR_3_PENALTY=9.610505 PRIMER_LEFT_3_PENALTY=7.394353 PRIMER_RIGHT_3_PENALTY=2.216152 PRIMER_LEFT_3_SEQUENCE=TCGCAATCAAATTTTTTCTATGACCTG PRIMER_RIGHT_3_SEQUENCE=GATCGGGAGCGTGCTTTG PRIMER_LEFT_3=37,27 PRIMER_RIGHT_3=136,18 PRIMER_LEFT_3_TM=60.394 PRIMER_RIGHT_3_TM=59.784 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=61.111 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=4.0000 PRIMER_RIGHT_3_END_STABILITY=2.7700 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=9.696873 PRIMER_LEFT_4_PENALTY=7.480721 PRIMER_RIGHT_4_PENALTY=2.216152 PRIMER_LEFT_4_SEQUENCE=ATCGCAATCAAATTTTTTCTATGACC PRIMER_RIGHT_4_SEQUENCE=GATCGGGAGCGTGCTTTG PRIMER_LEFT_4=36,26 PRIMER_RIGHT_4=136,18 PRIMER_LEFT_4_TM=58.519 PRIMER_RIGHT_4_TM=59.784 PRIMER_LEFT_4_GC_PERCENT=30.769 PRIMER_RIGHT_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=4.0200 PRIMER_RIGHT_4_END_STABILITY=2.7700 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=101 = SEQUENCE_ID=SP01-15.txt SEQUENCE_TEMPLATE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=SP01-19.txt SEQUENCE_TEMPLATE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.078669 PRIMER_LEFT_0_PENALTY=0.048989 PRIMER_RIGHT_0_PENALTY=0.029680 PRIMER_LEFT_0_SEQUENCE=AACCTGGCTACAGTTCCCTC PRIMER_RIGHT_0_SEQUENCE=CAGTGCTCTTTCTGCCAGTG PRIMER_LEFT_0=72,20 PRIMER_RIGHT_0=176,20 PRIMER_LEFT_0_TM=59.951 PRIMER_RIGHT_0_TM=60.030 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=43.93 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.3000 PRIMER_RIGHT_0_END_STABILITY=3.6600 PRIMER_PAIR_0_COMPL_ANY_TH=2.45 PRIMER_PAIR_0_COMPL_END_TH=2.46 PRIMER_PAIR_0_PRODUCT_SIZE=105 PRIMER_PAIR_1_PENALTY=0.078961 PRIMER_LEFT_1_PENALTY=0.048989 PRIMER_RIGHT_1_PENALTY=0.029971 PRIMER_LEFT_1_SEQUENCE=AACCTGGCTACAGTTCCCTC PRIMER_RIGHT_1_SEQUENCE=GCTCTTTCTGCCAGTGTCAC PRIMER_LEFT_1=72,20 PRIMER_RIGHT_1=172,20 PRIMER_LEFT_1_TM=59.951 PRIMER_RIGHT_1_TM=60.030 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=43.93 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=4.3000 PRIMER_RIGHT_1_END_STABILITY=3.6700 PRIMER_PAIR_1_COMPL_ANY_TH=5.96 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=101 PRIMER_PAIR_2_PENALTY=0.078961 PRIMER_LEFT_2_PENALTY=0.048989 PRIMER_RIGHT_2_PENALTY=0.029971 PRIMER_LEFT_2_SEQUENCE=AACCTGGCTACAGTTCCCTC PRIMER_RIGHT_2_SEQUENCE=GTGCTCTTTCTGCCAGTGTC PRIMER_LEFT_2=72,20 PRIMER_RIGHT_2=174,20 PRIMER_LEFT_2_TM=59.951 PRIMER_RIGHT_2_TM=60.030 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=43.93 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=4.3000 PRIMER_RIGHT_2_END_STABILITY=3.6700 PRIMER_PAIR_2_COMPL_ANY_TH=5.96 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=103 PRIMER_PAIR_3_PENALTY=0.233107 PRIMER_LEFT_3_PENALTY=0.048989 PRIMER_RIGHT_3_PENALTY=0.184118 PRIMER_LEFT_3_SEQUENCE=AACCTGGCTACAGTTCCCTC PRIMER_RIGHT_3_SEQUENCE=CATCCAGTGCTCTTTCTGCC PRIMER_LEFT_3=72,20 PRIMER_RIGHT_3=180,20 PRIMER_LEFT_3_TM=59.951 PRIMER_RIGHT_3_TM=59.816 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=43.93 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=4.3000 PRIMER_RIGHT_3_END_STABILITY=4.8500 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=109 PRIMER_PAIR_4_PENALTY=0.291862 PRIMER_LEFT_4_PENALTY=0.048989 PRIMER_RIGHT_4_PENALTY=0.242873 PRIMER_LEFT_4_SEQUENCE=AACCTGGCTACAGTTCCCTC PRIMER_RIGHT_4_SEQUENCE=ATCCAGTGCTCTTTCTGCCA PRIMER_LEFT_4=72,20 PRIMER_RIGHT_4=179,20 PRIMER_LEFT_4_TM=59.951 PRIMER_RIGHT_4_TM=60.243 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=43.93 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=4.3000 PRIMER_RIGHT_4_END_STABILITY=4.9200 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=108 = SEQUENCE_ID=SP01-20.txt SEQUENCE_TEMPLATE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=SP01-21.txt SEQUENCE_TEMPLATE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.710044 PRIMER_LEFT_0_PENALTY=0.024450 PRIMER_RIGHT_0_PENALTY=0.685594 PRIMER_LEFT_0_SEQUENCE=TGTGTCTTGTGGGTGTGTCT PRIMER_RIGHT_0_SEQUENCE=ACACACACACACAACACACA PRIMER_LEFT_0=59,20 PRIMER_RIGHT_0=159,20 PRIMER_LEFT_0_TM=60.024 PRIMER_RIGHT_0_TM=59.314 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.4100 PRIMER_RIGHT_0_END_STABILITY=3.7200 PRIMER_PAIR_0_COMPL_ANY_TH=40.01 PRIMER_PAIR_0_COMPL_END_TH=38.93 PRIMER_PAIR_0_PRODUCT_SIZE=101 PRIMER_PAIR_1_PENALTY=1.080122 PRIMER_LEFT_1_PENALTY=0.394528 PRIMER_RIGHT_1_PENALTY=0.685594 PRIMER_LEFT_1_SEQUENCE=GTGTCTTGTGGGTGTGTCTG PRIMER_RIGHT_1_SEQUENCE=ACACACACACACAACACACA PRIMER_LEFT_1=60,20 PRIMER_RIGHT_1=159,20 PRIMER_LEFT_1_TM=59.605 PRIMER_RIGHT_1_TM=59.314 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=3.5100 PRIMER_RIGHT_1_END_STABILITY=3.7200 PRIMER_PAIR_1_COMPL_ANY_TH=38.19 PRIMER_PAIR_1_COMPL_END_TH=35.05 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=1.129499 PRIMER_LEFT_2_PENALTY=0.024450 PRIMER_RIGHT_2_PENALTY=1.105049 PRIMER_LEFT_2_SEQUENCE=TGTCTTGTGGGTGTGTCTGT PRIMER_RIGHT_2_SEQUENCE=AGACACACACACACAACACAC PRIMER_LEFT_2=61,20 PRIMER_RIGHT_2=161,21 PRIMER_LEFT_2_TM=60.024 PRIMER_RIGHT_2_TM=60.105 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=3.4100 PRIMER_RIGHT_2_END_STABILITY=3.8200 PRIMER_PAIR_2_COMPL_ANY_TH=31.60 PRIMER_PAIR_2_COMPL_END_TH=34.49 PRIMER_PAIR_2_PRODUCT_SIZE=101 PRIMER_PAIR_3_PENALTY=1.129499 PRIMER_LEFT_3_PENALTY=0.024450 PRIMER_RIGHT_3_PENALTY=1.105049 PRIMER_LEFT_3_SEQUENCE=TGTCTTGTGGGTGTGTCTGT PRIMER_RIGHT_3_SEQUENCE=ACAGACACACACACACAACAC PRIMER_LEFT_3=61,20 PRIMER_RIGHT_3=163,21 PRIMER_LEFT_3_TM=60.024 PRIMER_RIGHT_3_TM=60.105 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=3.4100 PRIMER_RIGHT_3_END_STABILITY=3.3200 PRIMER_PAIR_3_COMPL_ANY_TH=33.14 PRIMER_PAIR_3_COMPL_END_TH=35.99 PRIMER_PAIR_3_PRODUCT_SIZE=103 PRIMER_PAIR_4_PENALTY=1.129499 PRIMER_LEFT_4_PENALTY=0.024450 PRIMER_RIGHT_4_PENALTY=1.105049 PRIMER_LEFT_4_SEQUENCE=TGTCTTGTGGGTGTGTCTGT PRIMER_RIGHT_4_SEQUENCE=ACACAACAGACACACACACAC PRIMER_LEFT_4=61,20 PRIMER_RIGHT_4=168,21 PRIMER_LEFT_4_TM=60.024 PRIMER_RIGHT_4_TM=60.105 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=3.4100 PRIMER_RIGHT_4_END_STABILITY=3.8200 PRIMER_PAIR_4_COMPL_ANY_TH=43.66 PRIMER_PAIR_4_COMPL_END_TH=43.66 PRIMER_PAIR_4_PRODUCT_SIZE=108 = SEQUENCE_ID=SP03-40.txt SEQUENCE_TEMPLATE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=SP04-07.txt SEQUENCE_TEMPLATE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.058082 PRIMER_LEFT_0_PENALTY=0.033010 PRIMER_RIGHT_0_PENALTY=0.025072 PRIMER_LEFT_0_SEQUENCE=CCGTGTACACACTGAACACG PRIMER_RIGHT_0_SEQUENCE=TGTGGCTAGTCATGGAGGTG PRIMER_LEFT_0=48,20 PRIMER_RIGHT_0=254,20 PRIMER_LEFT_0_TM=60.033 PRIMER_RIGHT_0_TM=60.025 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=25.52 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=20.62 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=44.85 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.4900 PRIMER_RIGHT_0_END_STABILITY=4.0000 PRIMER_PAIR_0_COMPL_ANY_TH=1.26 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=207 PRIMER_PAIR_1_PENALTY=0.080334 PRIMER_LEFT_1_PENALTY=0.033010 PRIMER_RIGHT_1_PENALTY=0.047323 PRIMER_LEFT_1_SEQUENCE=CCGTGTACACACTGAACACG PRIMER_RIGHT_1_SEQUENCE=TCCCACAGCTGTAGGAAGTG PRIMER_LEFT_1=48,20 PRIMER_RIGHT_1=216,20 PRIMER_LEFT_1_TM=60.033 PRIMER_RIGHT_1_TM=59.953 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY_TH=25.52 PRIMER_RIGHT_1_SELF_ANY_TH=18.98 PRIMER_LEFT_1_SELF_END_TH=20.62 PRIMER_RIGHT_1_SELF_END_TH=7.67 PRIMER_LEFT_1_HAIRPIN_TH=44.85 PRIMER_RIGHT_1_HAIRPIN_TH=38.58 PRIMER_LEFT_1_END_STABILITY=4.4900 PRIMER_RIGHT_1_END_STABILITY=3.1600 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=169 PRIMER_PAIR_2_PENALTY=0.081847 PRIMER_LEFT_2_PENALTY=0.033010 PRIMER_RIGHT_2_PENALTY=0.048837 PRIMER_LEFT_2_SEQUENCE=CCGTGTACACACTGAACACG PRIMER_RIGHT_2_SEQUENCE=TCTCCTAGCTGGGTTTGTGG PRIMER_LEFT_2=48,20 PRIMER_RIGHT_2=269,20 PRIMER_LEFT_2_TM=60.033 PRIMER_RIGHT_2_TM=59.951 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY_TH=25.52 PRIMER_RIGHT_2_SELF_ANY_TH=11.17 PRIMER_LEFT_2_SELF_END_TH=20.62 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=44.85 PRIMER_RIGHT_2_HAIRPIN_TH=36.53 PRIMER_LEFT_2_END_STABILITY=4.4900 PRIMER_RIGHT_2_END_STABILITY=4.1700 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=222 PRIMER_PAIR_3_PENALTY=0.132102 PRIMER_LEFT_3_PENALTY=0.033010 PRIMER_RIGHT_3_PENALTY=0.099091 PRIMER_LEFT_3_SEQUENCE=CCGTGTACACACTGAACACG PRIMER_RIGHT_3_SEQUENCE=GAGCCTCATTTTCCCACAGC PRIMER_LEFT_3=48,20 PRIMER_RIGHT_3=227,20 PRIMER_LEFT_3_TM=60.033 PRIMER_RIGHT_3_TM=60.099 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY_TH=25.52 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=20.62 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=44.85 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=4.4900 PRIMER_RIGHT_3_END_STABILITY=4.4000 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=180 PRIMER_PAIR_4_PENALTY=0.205019 PRIMER_LEFT_4_PENALTY=0.179947 PRIMER_RIGHT_4_PENALTY=0.025072 PRIMER_LEFT_4_SEQUENCE=CCGCCATGTACACACTGAAG PRIMER_RIGHT_4_SEQUENCE=TGTGGCTAGTCATGGAGGTG PRIMER_LEFT_4=96,20 PRIMER_RIGHT_4=254,20 PRIMER_LEFT_4_TM=59.820 PRIMER_RIGHT_4_TM=60.025 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=3.0200 PRIMER_RIGHT_4_END_STABILITY=4.0000 PRIMER_PAIR_4_COMPL_ANY_TH=16.34 PRIMER_PAIR_4_COMPL_END_TH=3.49 PRIMER_PAIR_4_PRODUCT_SIZE=159 = SEQUENCE_ID=AGAG SEQUENCE_TEMPLATE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=long_seq SEQUENCE_TEMPLATE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.128531 PRIMER_LEFT_0_PENALTY=0.028008 PRIMER_RIGHT_0_PENALTY=0.100522 PRIMER_LEFT_0_SEQUENCE=tgctagctaggcgatgctag PRIMER_RIGHT_0_SEQUENCE=ccgctacgatcgatcagcta PRIMER_LEFT_0=411,20 PRIMER_RIGHT_0=584,20 PRIMER_LEFT_0_TM=60.028 PRIMER_RIGHT_0_TM=60.101 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=23.16 PRIMER_RIGHT_0_SELF_ANY_TH=26.08 PRIMER_LEFT_0_SELF_END_TH=23.16 PRIMER_RIGHT_0_SELF_END_TH=14.12 PRIMER_LEFT_0_HAIRPIN_TH=38.59 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.4200 PRIMER_RIGHT_0_END_STABILITY=3.3200 PRIMER_PAIR_0_COMPL_ANY_TH=7.52 PRIMER_PAIR_0_COMPL_END_TH=6.37 PRIMER_PAIR_0_PRODUCT_SIZE=174 PRIMER_PAIR_1_PENALTY=0.143566 PRIMER_LEFT_1_PENALTY=0.043044 PRIMER_RIGHT_1_PENALTY=0.100522 PRIMER_LEFT_1_SEQUENCE=actgatacgcgatgctagct PRIMER_RIGHT_1_SEQUENCE=ccgctacgatcgatcagcta PRIMER_LEFT_1=476,20 PRIMER_RIGHT_1=584,20 PRIMER_LEFT_1_TM=59.957 PRIMER_RIGHT_1_TM=60.101 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY_TH=17.69 PRIMER_RIGHT_1_SELF_ANY_TH=26.08 PRIMER_LEFT_1_SELF_END_TH=1.35 PRIMER_RIGHT_1_SELF_END_TH=14.12 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=3.3200 PRIMER_RIGHT_1_END_STABILITY=3.3200 PRIMER_PAIR_1_COMPL_ANY_TH=15.45 PRIMER_PAIR_1_COMPL_END_TH=2.99 PRIMER_PAIR_1_PRODUCT_SIZE=109 PRIMER_PAIR_2_PENALTY=0.200309 PRIMER_LEFT_2_PENALTY=0.099786 PRIMER_RIGHT_2_PENALTY=0.100522 PRIMER_LEFT_2_SEQUENCE=gatcgatgctagctaggcga PRIMER_RIGHT_2_SEQUENCE=ccgctacgatcgatcagcta PRIMER_LEFT_2=405,20 PRIMER_RIGHT_2=584,20 PRIMER_LEFT_2_TM=60.100 PRIMER_RIGHT_2_TM=60.101 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY_TH=16.30 PRIMER_RIGHT_2_SELF_ANY_TH=26.08 PRIMER_LEFT_2_SELF_END_TH=16.30 PRIMER_RIGHT_2_SELF_END_TH=14.12 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=5.5400 PRIMER_RIGHT_2_END_STABILITY=3.3200 PRIMER_PAIR_2_COMPL_ANY_TH=18.59 PRIMER_PAIR_2_COMPL_END_TH=18.43 PRIMER_PAIR_2_PRODUCT_SIZE=180 PRIMER_PAIR_3_PENALTY=0.200309 PRIMER_LEFT_3_PENALTY=0.099786 PRIMER_RIGHT_3_PENALTY=0.100522 PRIMER_LEFT_3_SEQUENCE=tcgatcgatgctagctaggc PRIMER_RIGHT_3_SEQUENCE=ccgctacgatcgatcagcta PRIMER_LEFT_3=403,20 PRIMER_RIGHT_3=584,20 PRIMER_LEFT_3_TM=60.100 PRIMER_RIGHT_3_TM=60.101 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY_TH=18.63 PRIMER_RIGHT_3_SELF_ANY_TH=26.08 PRIMER_LEFT_3_SELF_END_TH=8.45 PRIMER_RIGHT_3_SELF_END_TH=14.12 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=3.9300 PRIMER_RIGHT_3_END_STABILITY=3.3200 PRIMER_PAIR_3_COMPL_ANY_TH=22.41 PRIMER_PAIR_3_COMPL_END_TH=19.54 PRIMER_PAIR_3_PRODUCT_SIZE=182 PRIMER_PAIR_4_PENALTY=0.295718 PRIMER_LEFT_4_PENALTY=0.252674 PRIMER_RIGHT_4_PENALTY=0.043044 PRIMER_LEFT_4_SEQUENCE=tatcatctctgcgcgatcga PRIMER_RIGHT_4_SEQUENCE=agctagcatcgcgtatcagt PRIMER_LEFT_4=361,20 PRIMER_RIGHT_4=495,20 PRIMER_LEFT_4_TM=59.747 PRIMER_RIGHT_4_TM=59.957 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY_TH=22.07 PRIMER_RIGHT_4_SELF_ANY_TH=19.30 PRIMER_LEFT_4_SELF_END_TH=1.72 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=38.48 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=3.5900 PRIMER_RIGHT_4_END_STABILITY=3.4100 PRIMER_PAIR_4_COMPL_ANY_TH=15.85 PRIMER_PAIR_4_COMPL_END_TH=7.27 PRIMER_PAIR_4_PRODUCT_SIZE=135 = SEQUENCE_ID=bad_letter SEQUENCE_TEMPLATE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC PRIMER_ERROR=Unrecognized base in input sequence = SEQUENCE_ID=bad_target SEQUENCE_TEMPLATE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC SEQUENCE_TARGET=51,10 SEQUENCE_TARGET=501,50 PRIMER_ERROR=TARGET beyond end of sequence = SEQUENCE_ID=other_bad_target SEQUENCE_TEMPLATE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC SEQUENCE_TARGET=101,1000 PRIMER_ERROR=TARGET beyond end of sequence = SEQUENCE_ID=included_test SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=21,228 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.710044 PRIMER_LEFT_0_PENALTY=0.024450 PRIMER_RIGHT_0_PENALTY=0.685594 PRIMER_LEFT_0_SEQUENCE=TGTGTCTTGTGGGTGTGTCT PRIMER_RIGHT_0_SEQUENCE=ACACACACACACAACACACA PRIMER_LEFT_0=79,20 PRIMER_RIGHT_0=179,20 PRIMER_LEFT_0_TM=60.024 PRIMER_RIGHT_0_TM=59.314 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.4100 PRIMER_RIGHT_0_END_STABILITY=3.7200 PRIMER_PAIR_0_COMPL_ANY_TH=40.01 PRIMER_PAIR_0_COMPL_END_TH=38.93 PRIMER_PAIR_0_PRODUCT_SIZE=101 PRIMER_PAIR_1_PENALTY=1.080122 PRIMER_LEFT_1_PENALTY=0.394528 PRIMER_RIGHT_1_PENALTY=0.685594 PRIMER_LEFT_1_SEQUENCE=GTGTCTTGTGGGTGTGTCTG PRIMER_RIGHT_1_SEQUENCE=ACACACACACACAACACACA PRIMER_LEFT_1=80,20 PRIMER_RIGHT_1=179,20 PRIMER_LEFT_1_TM=59.605 PRIMER_RIGHT_1_TM=59.314 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=3.5100 PRIMER_RIGHT_1_END_STABILITY=3.7200 PRIMER_PAIR_1_COMPL_ANY_TH=38.19 PRIMER_PAIR_1_COMPL_END_TH=35.05 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=1.129499 PRIMER_LEFT_2_PENALTY=0.024450 PRIMER_RIGHT_2_PENALTY=1.105049 PRIMER_LEFT_2_SEQUENCE=TGTCTTGTGGGTGTGTCTGT PRIMER_RIGHT_2_SEQUENCE=AGACACACACACACAACACAC PRIMER_LEFT_2=81,20 PRIMER_RIGHT_2=181,21 PRIMER_LEFT_2_TM=60.024 PRIMER_RIGHT_2_TM=60.105 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=3.4100 PRIMER_RIGHT_2_END_STABILITY=3.8200 PRIMER_PAIR_2_COMPL_ANY_TH=31.60 PRIMER_PAIR_2_COMPL_END_TH=34.49 PRIMER_PAIR_2_PRODUCT_SIZE=101 PRIMER_PAIR_3_PENALTY=1.129499 PRIMER_LEFT_3_PENALTY=0.024450 PRIMER_RIGHT_3_PENALTY=1.105049 PRIMER_LEFT_3_SEQUENCE=TGTCTTGTGGGTGTGTCTGT PRIMER_RIGHT_3_SEQUENCE=ACAGACACACACACACAACAC PRIMER_LEFT_3=81,20 PRIMER_RIGHT_3=183,21 PRIMER_LEFT_3_TM=60.024 PRIMER_RIGHT_3_TM=60.105 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=3.4100 PRIMER_RIGHT_3_END_STABILITY=3.3200 PRIMER_PAIR_3_COMPL_ANY_TH=33.14 PRIMER_PAIR_3_COMPL_END_TH=35.99 PRIMER_PAIR_3_PRODUCT_SIZE=103 PRIMER_PAIR_4_PENALTY=1.129499 PRIMER_LEFT_4_PENALTY=0.024450 PRIMER_RIGHT_4_PENALTY=1.105049 PRIMER_LEFT_4_SEQUENCE=TGTCTTGTGGGTGTGTCTGT PRIMER_RIGHT_4_SEQUENCE=ACACAACAGACACACACACAC PRIMER_LEFT_4=81,20 PRIMER_RIGHT_4=188,21 PRIMER_LEFT_4_TM=60.024 PRIMER_RIGHT_4_TM=60.105 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=3.4100 PRIMER_RIGHT_4_END_STABILITY=3.8200 PRIMER_PAIR_4_COMPL_ANY_TH=43.66 PRIMER_PAIR_4_COMPL_END_TH=43.66 PRIMER_PAIR_4_PRODUCT_SIZE=108 = PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MAX_SELF_END=8 SEQUENCE_ID=included_test_w_middle SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=21,228 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.286428 PRIMER_LEFT_0_PENALTY=0.024450 PRIMER_RIGHT_0_PENALTY=1.261978 PRIMER_INTERNAL_0_PENALTY=9.546432 PRIMER_LEFT_0_SEQUENCE=TGTCTTGTGGGTGTGTCTGT PRIMER_RIGHT_0_SEQUENCE=ACACAACAGACACACACACA PRIMER_INTERNAL_0_SEQUENCE=TGGATGTAGCTTCTTGACTGTGTGTTG PRIMER_LEFT_0=81,20 PRIMER_RIGHT_0=188,20 PRIMER_INTERNAL_0=142,27 PRIMER_LEFT_0_TM=60.024 PRIMER_RIGHT_0_TM=58.738 PRIMER_INTERNAL_0_TM=57.454 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=44.444 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.4100 PRIMER_RIGHT_0_END_STABILITY=3.7200 PRIMER_PAIR_0_COMPL_ANY_TH=43.66 PRIMER_PAIR_0_COMPL_END_TH=43.66 PRIMER_PAIR_0_PRODUCT_SIZE=108 PRIMER_PAIR_1_PENALTY=1.286428 PRIMER_LEFT_1_PENALTY=0.024450 PRIMER_RIGHT_1_PENALTY=1.261978 PRIMER_INTERNAL_1_PENALTY=9.546432 PRIMER_LEFT_1_SEQUENCE=TGTGTCTTGTGGGTGTGTCT PRIMER_RIGHT_1_SEQUENCE=ACACAACAGACACACACACA PRIMER_INTERNAL_1_SEQUENCE=TGGATGTAGCTTCTTGACTGTGTGTTG PRIMER_LEFT_1=79,20 PRIMER_RIGHT_1=188,20 PRIMER_INTERNAL_1=142,27 PRIMER_LEFT_1_TM=60.024 PRIMER_RIGHT_1_TM=58.738 PRIMER_INTERNAL_1_TM=57.454 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=44.444 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=3.4100 PRIMER_RIGHT_1_END_STABILITY=3.7200 PRIMER_PAIR_1_COMPL_ANY_TH=35.43 PRIMER_PAIR_1_COMPL_END_TH=35.43 PRIMER_PAIR_1_PRODUCT_SIZE=110 PRIMER_PAIR_2_PENALTY=1.656507 PRIMER_LEFT_2_PENALTY=0.394528 PRIMER_RIGHT_2_PENALTY=1.261978 PRIMER_INTERNAL_2_PENALTY=9.546432 PRIMER_LEFT_2_SEQUENCE=GTGTCTTGTGGGTGTGTCTG PRIMER_RIGHT_2_SEQUENCE=ACACAACAGACACACACACA PRIMER_INTERNAL_2_SEQUENCE=TGGATGTAGCTTCTTGACTGTGTGTTG PRIMER_LEFT_2=80,20 PRIMER_RIGHT_2=188,20 PRIMER_INTERNAL_2=142,27 PRIMER_LEFT_2_TM=59.605 PRIMER_RIGHT_2_TM=58.738 PRIMER_INTERNAL_2_TM=57.454 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=44.444 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=3.5100 PRIMER_RIGHT_2_END_STABILITY=3.7200 PRIMER_PAIR_2_COMPL_ANY_TH=42.98 PRIMER_PAIR_2_COMPL_END_TH=42.98 PRIMER_PAIR_2_PRODUCT_SIZE=109 PRIMER_PAIR_3_PENALTY=2.382988 PRIMER_LEFT_3_PENALTY=1.121010 PRIMER_RIGHT_3_PENALTY=1.261978 PRIMER_INTERNAL_3_PENALTY=9.546432 PRIMER_LEFT_3_SEQUENCE=GTCTTGTGGGTGTGTCTGTT PRIMER_RIGHT_3_SEQUENCE=ACACAACAGACACACACACA PRIMER_INTERNAL_3_SEQUENCE=TGGATGTAGCTTCTTGACTGTGTGTTG PRIMER_LEFT_3=82,20 PRIMER_RIGHT_3=188,20 PRIMER_INTERNAL_3=142,27 PRIMER_LEFT_3_TM=58.879 PRIMER_RIGHT_3_TM=58.738 PRIMER_INTERNAL_3_TM=57.454 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=44.444 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=3.1600 PRIMER_RIGHT_3_END_STABILITY=3.7200 PRIMER_PAIR_3_COMPL_ANY_TH=45.65 PRIMER_PAIR_3_COMPL_END_TH=45.65 PRIMER_PAIR_3_PRODUCT_SIZE=107 PRIMER_PAIR_4_PENALTY=2.425217 PRIMER_LEFT_4_PENALTY=0.024450 PRIMER_RIGHT_4_PENALTY=2.400767 PRIMER_INTERNAL_4_PENALTY=9.546432 PRIMER_LEFT_4_SEQUENCE=TGTCTTGTGGGTGTGTCTGT PRIMER_RIGHT_4_SEQUENCE=TACACAACAGACACACACACA PRIMER_INTERNAL_4_SEQUENCE=TGGATGTAGCTTCTTGACTGTGTGTTG PRIMER_LEFT_4=81,20 PRIMER_RIGHT_4=189,21 PRIMER_INTERNAL_4=142,27 PRIMER_LEFT_4_TM=60.024 PRIMER_RIGHT_4_TM=58.599 PRIMER_INTERNAL_4_TM=57.454 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=44.444 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=3.4100 PRIMER_RIGHT_4_END_STABILITY=3.7200 PRIMER_PAIR_4_COMPL_ANY_TH=43.66 PRIMER_PAIR_4_COMPL_END_TH=43.66 PRIMER_PAIR_4_PRODUCT_SIZE=109 = primer3-2.3.6/test/primer_position_penalty_input0000664000077300007730000014610512231156245021164 0ustar ioanaioanaPRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=36-2000 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_GC_CLAMP=0 PRIMER_OPT_SIZE=20 PRIMER_MIN_SIZE=18 PRIMER_MAX_SIZE=27 PRIMER_OPT_TM=60.0 PRIMER_MIN_TM=57.0 PRIMER_MAX_TM=63.0 PRIMER_PAIR_MAX_DIFF_TM=100.0 PRIMER_MIN_GC=20.0 PRIMER_MAX_GC=80.0 PRIMER_SALT_MONOVALENT=50.0 PRIMER_DNA_CONC=50.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_SELF_ANY=8.00 PRIMER_MAX_SELF_END=3.00 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_MAX_POLY_X=5 PRIMER_LIBERAL_BASE=0 PRIMER_NUM_RETURN=1 PRIMER_FIRST_BASE_INDEX=0 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_MAX_LIBRARY_MISPRIMING=11.00 PRIMER_OUTSIDE_PENALTY=20.0 PRIMER_INSIDE_PENALTY=1000.0 SEQUENCE_ID=AT3_X1 SEQUENCE_TARGET=298,45 SEQUENCE_TEMPLATE=TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTCCCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACTTTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCTCTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCAGGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTCCCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAAGGACTCACAGGAATGACCTCCAACACCTTTGAGAAGACCAGGCCCTCTCCCTGGTAGTTACAGTCAAAGACCTGTTTGGAAGACGTCATTTCAAGTGCTCTCCCTCCCACCCCACCTCTTGGGGTAAGGCCTTTCCTAAGCTACCCCTTGGGTCCCTAGCCTAAGAAACAAGGGGGATGTCATCCCTGGTGTAAAGATGCTGTGCAGGAAGTCAGCACTCACGGGATCCAGGGGACGCTCCAAG = SEQUENCE_ID=AT3_X2 SEQUENCE_TARGET=298,371 SEQUENCE_TEMPLATE=AAACAGGAAGCTTCTCATTCTTTTTTCTCCCAGCGTGGGGAATTGAGCACATAATACTCCAAATAACCATCAGATGATTCTAATTCCAACATGACCACGTCCAGGCAACTGAACTGTCCCCTGGCAAGAAGTCTAGGACTGAACCTGTCCCGGGCCCCTGTACTTGGTTCAAAGGATTTAGCCTTTCTCTTGGCCACACCAGGTGGGCTGGAATCCTCTGCTTTACTGGGGCAACCCTGTGGTGGGCAGTGGGGCTAGGGGTTGCAGCCTAGCTTAACTTGGCATTTTGTCTCCTTGCAGGAAGGTTTATCTTTTGTCCTTGCTGCTCATTGGCTTCTGGGACTGCGTGACCTGTCACGGGAGCCCTGTGGACATCTGCACAGCCAAGCCGCGGGACATTCCCATGAATCCCATGTGCATTTACCGCTCCCCGGAGAAGAAGGCAACTGAGGATGAGGGCTCAGAACAGAAGATCCCGGAGGCCACCAACCGGCGTGTCTGGGAACTGTCCAAGGCCAATTCCCGCTTTGCTACCACTTTCTATCAGCACCTGGCAGATTCCAAGAATGACAATGATAACATTTTCCTGTCACCCCTGAGTATCTCCACGGCTTTTGCTATGACCAAGCTGGGTGCCTGTAATGACACCCTCCAGCAACTGATGGAGGTACGACCAAAGGTCTTCTGCCCAGCCACCTTGTTAGGAGCACCTTTGGGCTTCCATAGGCCCAAGTCCAATGATTCCTCAACCAACACTGCAGCCACTAGGGGCGCTCATTATGCATTACGATTCCCTTTGAACATCACTGTGTTATAATTCCCTTTGAAAATCATTTTTTAAAAAATTAGCCAAGGAATCTTGGCTATCTACTTTTTAAATCCTGGTTTCCTCTTTTGAGCACCTTAAAATGGGGGAAGGCTTGTATCTTCTCTCAACTTCTTTTCAGTAATTCTTTCATCTATATGT = SEQUENCE_ID=AT3_X3 SEQUENCE_TARGET=298,220 SEQUENCE_TEMPLATE=CAAGAGGACTTTTCCCATTCCAGCAAGAAAACGTCTGTGTGTTGATCTAGAGGCGTTTAGAGACTTTAGGTGGCAACCTAGTCTCTCTTTTTCCCTTTATCCTTCCTACCCTTCATTCTTCTTTTATCCTTTTATTCATCAGAACACAAGAGTTGAGCATTTATGCTGTCCCAGGTACTGTGCTTGAAGGAGTTAACAACTGAGGTGGCTATTAGTCAGAGACTGACCAGCATGTGCTCACCACCCATGTTAACTAGGCAGCCCACCAAACCCACCACCATTTTTTTTTGACTTCTATAGGTATTTAAGTTTGACACCATATCTGAGAAAACATCTGATCAGATCCACTTCTTCTTTGCCAAACTGAACTGCCGACTCTATCGAAAAGCCAACAAATCCTCCAAGTTAGTATCAGCCAATCGCCTTTTTGGAGACAAATCCCTTACCTTCAATGAGACCTACCAGGACATCAGTGAGTTGGTATATGGAGCCAAGCTCCAGCCCCTGGACTTCAAGGTGAGTTGCAGATGTTACCCCTGACCTCCGAGTTCTTCCTCTCCACTCAGAGATTGAGGAGGTGGAGAAACAGCATCCAAATTCACACTGCTTTGCTGCTGAAGACTGCTGGAGGGCTGACTAAAAGTTAGAACCCCTGCAATAGTTATTCTTACTTGAAACCTGAGAAATCAAAGGTATCCATGCTTGGATTGTAGTGACTGCCCAGAAAACATGAATTAATAATCAATTCTTCATTCCATCCACCAACTTCAAATATATACCAAAGGGTGTTTTGAAGATGCCAGTTCTACAAGATAT = SEQUENCE_ID=AT3_X3' SEQUENCE_TARGET=298,142 SEQUENCE_TEMPLATE=AGTCAGAGAAAATAATATTTTATCTTCTTTTTCCCAGACTATTTTCGCCTTCCTTCTTTTCATTTATTTCTTCCTATTTCTTTTTGTCTTTTTCTTCTGATAATATTTATTAACTACAGGAAAGATTCATGGAACTATATTAGATATGTGAGGCTTCCCAATTTGGGTTAGAGCAATGGCTTCTTAATCAAATGGTGGGAAAGGACAGAGGGATGGTGAGAAAAATAAAATGCTGCCTGGGAAAATGGAGAAGCCAATTGAATAGCACAGGTGAGTAGGTTTATTTTCTGTTCTCCTCAGGAAAATGCAGAGCAATCCAGAGCGGCCATCAACAAATGGGTGTCCAATAAGACCGAAGGCCGAATCACCGATGTCATTCCCTCGGAAGCCATCAATGAGCTCACTGTTCTGGTGCTGGTTAACACCATTTACTTCAAGGTACTCAGAATGGCCCTGGAGAGACCCCAGGGACTTCCTCTTGCTCTTCAGCTTACCCCCTTTTTTTTTAAATGGCGAGACCGAAGCCCTGAGAGGGCAAATGGACTGCCGAAAGCTACACAGGTACAGGTCAGCAGGGCAGGTCAATCTATTATTTATTTATTTATTTATTTTTGACAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAAGCTTCGCCTCCTGGGTTCTCGGCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGAATACAGGC = SEQUENCE_ID=AT3_X4 SEQUENCE_TARGET=298,395 SEQUENCE_TEMPLATE=GGCTTTGTCACTCATTTCTTCTTAGCATGGAATAGCATTACATTTGGTCTGGATGTACCACAGTCTGTCTATTCATCTACTGAAGGACATTTTGGCTGCTTCCAAGGTTTGACAGTTATGAATAAACCTACTCATAATTCCATCATTCTGACACAGCCATTGTTAACCTTTTTGTGCATATCCCGCCAGTCTTTTTTCCGAATAATTATATATTAATGTAACACTATAATATGGATATGTCTGTGTCAATAACTATCCTCCTATGAATGTTTGTGTTCTTACTTTGTGATTCTCTTCCAGGGCCTGTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGGAACTGTTCTACAAGGCTGATGGAGAGTCGTGTTCAGCATCTATGATGTACCAGGAAGGCAAGTTCCGTTATCGGCGCGTGGCTGAAGGCACCCAGGTGCTTGAGTTGCCCTTCAAAGGTGATGACATCACCATGGTCCTCATCTTGCCCAAGCCTGAGAAGAGCCTGGCCAAGGTGGAGAAGGAACTCACCCCAGAGGTGCTGCAGGAGTGGCTGGATGAATTGGAGGAGATGATGCTGGTGGTCCACATGCCCCGCTTCCGCATTGAGGACGGCTTCAGTTTGAAGGAGCAGCTGCAAGACATGGGCCTTGTCGATCTGTTCAGCCCTGAAAAGTCCAAACTCCCAGGTTTGTCTAGGAAGGAGTTTCCTCCCTTCTCCACCCGCAAGGTAGTCTGACCAAAAGTGGAAGAGTTGGAGAAAGAATAGAAAGGAGCAACAAGTCAGGACTCCTGGATACTGATCCTAGTTTCTACTGCTAATTTGTGGAAATCTCTTTTCCTTTTGAGACCTCAGTTTCCTCTTCTGTAAAAGGGAAGTTTGTTCTTGGATCTCCATGGGCCCAGCCAGCACTGGTGCCCTGTGAGTCTGTATCAGGTAGAGGAGATGGGACCAGGTGGAGAGGAATTTGAAAGGGCATTGGAATTCA = SEQUENCE_ID=AT3_X5 SEQUENCE_TARGET=298,69 SEQUENCE_TEMPLATE=TAGAGACAAGGTTTCACCATATTAGTTAGAGTGTCTCCAACTCCTGACCTCAGGTGATCTGTACACCTTGGCCTCCCGAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCCCCAAAGTACTTTATTATTTTTAACACATATTCATTGTGAGAGTATGATTAGGTGAAGATTTAGGATTTCTTCTTATGTTTCAAAAAGCCCCAAAGGATCTCTTAATCCAAACTGAATTCCCATCTGTGGATTGAAGCCAACTTTCTCCCATCTCACAAAGACTTCTCCGGTCTTCCTTCCAGGTATTGTTGCAGAAGGCCGAGATGACCTCTATGTCTCAGATGCATTCCATAAGGCATTTCTTGAGGTGAGTACACCTTCCCCACTCTCTTAGGGTACAGAAAGGAGATGCATGAACAGCAGGAACACGTGGAAAAGGCCTGTTTCCAGTGTTAAGGCATGCAAAAGGCCTCCACAGGCTGCTATAATACAGCCCTCTCCAAAACCTTCATGGTGTGATTGTTCTGCCTTCCCTCCCACTACCTCTTCTGTAGCAGGTCAAGCGGGAACACAAACATTTAGGGAGGGTGATATAGGAAAAGAAGCCAGCAAAGGCCATCAAGAAGAAATTTACAGCATGAGGAGAACCAGAAGAGTATGGGGTCGC = SEQUENCE_ID=AT3_X6 SEQUENCE_TARGET=298,181 SEQUENCE_TEMPLATE=CCATTTACAATGGTACCAGCCAGAAATGTGATAACCTGTGTACATTCAGATTTCTGGGTTACCTGAATGGAACTCTTACACTTATTACCTAGCACAAGGCTTGGACAAACACAAGTACCTTACATTCTCTGCATGAAAGAATGAGTGAAAGTAGGATTCTGGAGGGAATCCAACCTGACCCAAATGTACTTTTTACTGGAAAACAAAAGCATTTGAGGAATTGCTGTGTCTGTGGATGATTTACCTGCCAAAATGAACGGCAGAGTGGCTAATTTAGTTTTATTCCCATGTGACCTGCAGGTAAATGAAGAAGGCAGTGAAGCAGCTGCAAGTACCGCTGTTGTGATTGCTGGCCGTTCGCTAAACCCCAACAGGGTGACTTTCAAGGCCAACAGGCCTTTCCTGGTTTTTATAAGAGAAGTTCCTCTGAACACTATTATCTTCATGGGCAGAGTAGCCAACCCTTGTGTTAAGTAAAATGTTCTTATTCTTTGCACCTCTTCCTATTTTTGGTTTGTGAACAGAAGTAAAAATAAATACAAACTACTTCCATCTCACATTATAAATGGACTCTGCATTTGAAATGAAGACAAGGAAAGGGGAAACATGCTATTGGGGCACATGGTAAAATGATGCCTTCAAGTTGTTCTTTACCCAGTAACCACATCTGGATCAAGAAAATGAGGGAGAGAGCGATAAAAGATGGTAGACAGCCAGAAAGGGAAGGGAGAG = SEQUENCE_ID=CETP_X1 SEQUENCE_TARGET=298,122 SEQUENCE_TEMPLATE=AGTAAAAATAAATGAATTCATGGAAACATATTAAGCAATTATCCAGATAACATAAGGGATGGCAAAAATGGTGCAGATGGTGGAGGGGAGACAAGTAGAAGTTGGGGTGCTCTTGTTGAATGTCTGGCTCTGAACTCTAGAGGAGGCCGCAGGGGCTGGGCAGGAAGGAGGTGAATCTCTGGGGCCAGGAAGACCCTGCTGCCCGGAAGAGCCTCATGTTCCGTGGGGGCTGGGCGGACATACATATACGGGCTCCAGGCTGAACGGCTCGGGCCACTTACACACCACTGCCTGATAACCATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAAGGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTGTAAGTATCAGTGCATCTGTCTGCCCTGCCAGGGGTCTTTTCATGGACACCCACTATGCCAGGAGCCTCCCTGGCCTGAAGCCAGCCCTGAAGCCGGCTGCCACACTAGCCCAGAGAGAGGAGTGCCCTGGGAGGGAGATGGGCTGAGTGGAGCTGTCATCACCCCCTCCTGACCTCGCCTTCAAGGTCAAGTTCTTTGGTGAGAAGGTCCTAGCTGCATTGCAAACAGCCAGGTATAGGGATTTGTGTTTGTCTGCGACCCAGAATCACTGGGGTTCGAGTTAGGGTTCAGATCTGAGC = SEQUENCE_ID=CETP_X2 SEQUENCE_TARGET=298,119 SEQUENCE_TEMPLATE=CTAGGGTTAGTGTGCGGCTGGGCTCAGGGCTGCCCCATTTGCTAGGATCGTGGGGTTCCCATGTGTCAGGATCCAGAGGCTAGGGTATGATCAGGATCTCTAGCTGGGGTCAGGGTCAGAGCTCTCTGTGTCCCCTAGAATTGCCATCAACCTTAAACCCAGAGGAGGCCCAGTCCAACCCCTCAGCTTTAAGACCTGGGAGCCTCATCTCAGAGAGGCTGAGTCATGGCCAAGGCAGTTGGGGTGGGAGCAGGGGGCTTGGTGTGGGCCTGCAGCCCTCATCCACTGCCCTCCCTCTAGTGAACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATATCACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAAGTGAGTCGGGCCTCGGGTGTGACCTGGCTGGGGGTAGGGTGGCGGGAGGAACAGCCTGGGCTTCCCCCAGCCACAGGGAGGAAAGGCAGCAGCTGGGGGACTCAGGTCTCTCCCCTTGATTTGGAACCAGAGCC = SEQUENCE_ID=CETP_X3 SEQUENCE_TARGET=298,139 SEQUENCE_TEMPLATE=TGCCTCCTCTTCCACTTTTCGGTACCCTGTGATTGATTGGGACCACCCAGATAACCTAGGATCATCTCCCCACCTACCCCAAGGTCCTTAACTTAACCATACTTCATATGGGTAACACGAGTTGAGTGTGGTACCCAGGTTTGACATGTTGGGTAACATATTTGCAGGTTCTGTGGATTAGGAGGACATTTTGGGGGCCATGATTCTATCTTCCACCCTCGCCTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGC = SEQUENCE_ID=CETP_X4 SEQUENCE_TARGET=298,75 SEQUENCE_TEMPLATE=CTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCAT = SEQUENCE_ID=CETP_X5 SEQUENCE_TARGET=298,92 SEQUENCE_TEMPLATE=GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCATCTCCAAGGGGAGCGAGAGTAAGTACACCACCCTGTGCCCCCATTCCTGTCGTGCCCATCCTGTTAGTGTGTCCACGGCCCCCTCCAGGCTCAACCCCACACAGGGATGCTTGTGGGTGGCCAAACCTGAGGGCAGCAATACCTTCAGTGGGGTCATTCCATCCCCCTCCATCAATACACCCTAAAGGCTGGAAACAACAATAACCAACAGCTAGTAACTAACAGCTATTAAGAACTTCTGTTGGCAAAGCACTATTCCAAGCCCTTTCATGAATTAATTGATTTTGTCCTTAAAACCAACCCTAGGATATAGATTCT = SEQUENCE_ID=CETP_X6 SEQUENCE_TARGET=298,74 SEQUENCE_TEMPLATE=TTTCTGACTCTGGAGCAAGACGGATACATGTATGAATTTGGACTCTAGACACGTTCTCGTGTGTGTGACAGGTGTGAGCGTCACAGGAGCTGGGCCCTCCCGAGGAATTCTGGATGGTGCCACAGTTAATTCTTGGGTCTGAGGCTCCGTGTTCTCACTGCAAAATGGGAGTGATAATTCTTACTTCCTGAGCTACAAGAGTCAGGGCCAACAGAGCCATGAAGGAGCCTGGTACACACTAGGCGCTCCATGGATGCACAGGACTGGTCAGGGGCTCATTGTGGTGCTTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCAT = SEQUENCE_ID=CETP_X7 SEQUENCE_TARGET=298,65 SEQUENCE_TEMPLATE=TTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCATGCCTCAGAAGACAGCAGTGGGAGCCAGAAAGCCACCTGCTGCACTATGTGGCCTTGGGACTGTCACTCTTCCTGTCTAGGTCCCATGGGCTCTATCTGGCTCTGACACTTGATGATTAGTTATGAGCATACTTTGGCAAAGCTCTGCCCCTTTGGTGCGGCTCACAAGCTGTGTGGCGAAGGGCTTGTCTATAGAACTCAGGACAAATGGGTGATTAAGTCCAAGAGGCATCCAAGATTCTCCTGGAAGTAGATTAGGAAAAAAGATAATTAGATTGC = SEQUENCE_ID=CETP_X8 SEQUENCE_TARGET=298,96 SEQUENCE_TEMPLATE=GCTGGGCACTCATCCATGTACTGTACTCTCCTATGCAGTACAGAGCAGAGCTGGGTTTCAGCCCAAGTCTTGGACTCTGCTCTGAACCAACCTTCTAGAAGGGCTCTACCTACCCAGACAGACAGACTTGGGAAAAGAGAGAATGAAAAAGTGCCACACCCCTCCCCGCACACCCAGGTCCCACTTTACAGAGGGGAACACTGAGGCTGGAGGGTTGGGTAGCTGTGTGGATGCAGGGGACGGTGACTCAGGGCAATTCCCCCATCCCTGAGGCCCTGCGTTGATCTTTTCCTCCTGCAGCCAGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCATCACAGCCTCCTACCTGGAGTCCCATCACAAGGTAGGAGTTGTGGGAGGGTGGGCAGGGCCCAGCTTCCCCAGGGGAGTTGGTCCTTTTTTGTGCTCTGACAACCCCATCCCCCAGCTTCAACCTTATGGCAGCCAAGAGTCCTGGGGAGCTCCTCCTCATTCCTGATGCTCCTCCGCATTCCTGATGCTGCGAGGAGGGCAGGCCACAGCGACGTGCCCCTGACCCCTCTCTGCAGGCACCAGGGCTGCCCACTACAAGGATCCCAGCAAAGCACCAGCTCCTTCCTAGAGGGCTTATTCGGCTTCTGTCATCCTCTACAGCAGTGGATTG = SEQUENCE_ID=CETP_X9 SEQUENCE_TARGET=163,184 SEQUENCE_TEMPLATE=TTTGTTGAATGAGTGAAAGCCGCTGGGGAAACTGGGTACAGCTCTTTCCTCAGTTTCCCCATCTGCACTCTGGGCTGAATGCTGGGCTCCTCCCAATCTCCCTGAAGCTGGACCTGAGCCCAGTAGGGACACACAGGGTCCAGCCAGCGTCCTGGCTTCCTCCAGGGTCATTTCATCTACAAGAATGTCTCAGAGGACCTCCCCCTCCCCACCTTCTCGCCCACACTGCTGGGGGACTCCCGCATGCTGTACTTCTGGTTCTCTGAGCGAGTCTTCCACTCGCTGGCCAAGGTAGCTTTCCAGGATGGCCGCCTCATGCTCAGCCTGATGGGAGACGAGTTCAAGGTGAGTGGGTGGGGCTGGGCTGCTAGGGGATCCAGATGGCATGTGGTATGTGTGTGTGTGCACACGCATGGGGAGGAGGGAGGAAACTCGGAAACTTGGTGGTGGGCAAAAGAACTAAGCTGGAGCAATAGCAGTGAAGTCCAGACTGGGCACAGTGGCTCACACCTGTAATCCCAATCCTTTGGGAGGCTGAGATGTAGCAGGACGAACCGCAGACAAAACTCCTCAGACACTGAGTTAAAGAAGGAAAGAGTTTATTCAGCCGGGAGCATGGGTAAGACTC = SEQUENCE_ID=CETP_X10 SEQUENCE_TARGET=235,55 SEQUENCE_TEMPLATE=CTTGTCGAGGTCACACAGCATGTGTGGGGCACAGTGGGGAGCAGAAGCCAGCCTCCAGCCGGACACCCCTTCCCCATTCCACACCCTGCCCAGAGCATCTCACATGTTGTCTGGGAGGTTGGGAGTTGCGTCTGAGGAGGGGTCCAGTCCTTGAAACTGCCCTTGGTCCCTGCGAAGTTTTCTTCTGAGGAGTGGACTTTACTCCACCCACCCTCCAACTTCCTCATTTCTTTTCAGGCAGTGCTGGAGACCTGGGGCTTCAACACCAACCAGGAAATCTTCCAAGAGGTAACTCCCCCCTGCCCCTGTGTGGGGTTTATCTCACGTACCCCAATCCTGCTCTGGCTTCAAGAGCCCCCACACAGCCAATAACACCACCAATGGCAACAATTATAACAG = SEQUENCE_ID=CETP_X11 SEQUENCE_TARGET=125,169 SEQUENCE_TEMPLATE=CCCCGAGCTACTTCCTTTTCCCAGCCCTGGGGCCCGGAGCCAGCTTTGTGGTTCCCATCTCCGAGGGCATGGACTGCTGCTCCGTGATGGGCCCCTGTCCTGGCCATGGGACCCCTGTCTTCCACAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTCCACTGCCTCAAGATGCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCAGTGATGGTGAAATTCCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTTGAAGAGGTGAGGCGGGTGCAGGGAGAGGTGGTGGTGGGGGAACTGACTCACATATGGGCCGCAGAGGGCAGGGGCTGGGGGTCTCTGAAGCCTCCAGATCCTTCTCACCACCTCTGCTGGCAC = SEQUENCE_ID=CETP_X12 SEQUENCE_TARGET=298,72 SEQUENCE_TEMPLATE=GTTGCAGTGAGCTGAGATCGTGCCACTGCCCTCCAGCCTGGGCAACAGAGTGAGACTGTCTCAAAAACAAAAAAAGAAAAGAAAAGAAAAAGAAAGTGACTTCTCAGGTCCTAACCCCAAAGCCACAGGTGCTGGGGAACTTTCCTCGGTTTTCAGAAGAGCAGTAGCTAAGCCTGGTTCCCGTGTCATCCTTGCCTCTCCAGTCCCTCAGTGGAAAGAATCAGGGGCCCTGAGCTAGGAGGGTTGCTCTCTGCTTCGGGAAGAGCCCTGGCTCACAGCAAATTTGGTTTCTCTCCCCAGGATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGCCTCTTGGATTTCCAGTATGTGCTGCAGAGAAGAGAAGGGGGCGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGT = SEQUENCE_ID=CETP_X13 SEQUENCE_TARGET=298,38 SEQUENCE_TEMPLATE=CGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGTCAAATTATCATCGCTTTTTTATTTCAGGATTACACCAAAGACTGTTTCCAACTTGACTGAGGTAGGTAGTCTTGGATAGACTGGGGGAAATAAGTCCTGTGGGACCTCCTGCCTTAAAGAAAGCAGGCGGAGGGCCCTAAAGGAAATCAGGCAACCAGACCAAAAGAATGTGACCAGGTGGTCCATGCTGTGTCTCTTGTGACCCTTCTTCTCCCTGCCATGTCTTTTGGGAGAGCCCTTGTGTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTC = SEQUENCE_ID=CETP_X14 SEQUENCE_TARGET=298,77 SEQUENCE_TEMPLATE=GTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTCCCCAGGGCGAAGGAAAGACCACTCAGGAAGGGCACCGTCTGGGGCAGGAAAACGGAGTGGGTTGGATGTATTTTTTTCACGGATGGGCATGAGGATGAATGCTTGTCCAGGCCGTGCAGCATCTGCCTTGTGGGTCACTTCTGTGCTCCAGGGAGGACTCACCATGGGCATTTGATTGCAGAGCAGCTCCGAGTCCGTCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCTGAGGTCATGTCTCGTAAGTGTGGGCTGGAGGGGAAACTGGGTGCCGAGGCTGACAGAGCTTCCCATTTCACCTTTT = SEQUENCE_ID=CETP_X15 SEQUENCE_TARGET=254,90 SEQUENCE_TEMPLATE=GGATGGGTTGGGAGCTCAAGTTTTGGGGCAGAAGGGAATTTTTTTTGGCAGCAGAGTGCAAGCCCTGCCGCCAGGCAAACTCTGCTCTTCCTCATCCTCAGAAGCACTTGCTCACTCTGCTAAATCAAAGTGAAACGCATGTTTACAGAATATTGGTCCAAAAGGGTCTCAGCATCTCCCACTACCCAGGGTGCAGAGCCTCGGGCCGGCCTTGCTCCCCAAGAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC = SEQUENCE_ID=CETP_X16 SEQUENCE_TARGET=298,79 SEQUENCE_TEMPLATE=GAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGCACCAGGCTCACAGCTGGAACCCTGGTGTCTCCTCCAGCGTGGTGGAAGTTGGGTTAGGAGTACGGAGATGGAGATTGGCTCCCAACTCCTCCCTATCCTAAAGGCCCACTGGCATTAAAGTGCTGTATCCAAGAGCTGCGGAGTCCTTCTTCTGTGGCTGGCGGGTAGAGGGGGGGGGAAGGGATTGTCTCACCAGTGCCGTCCACCTCTTTTCAGCCCTTCCAAGCAGCTGCCCCCAAACCCTCCAAGCTT = SEQUENCE_ID=F2_X1 SEQUENCE_TARGET=298,114 SEQUENCE_TEMPLATE=AAGCAACCACCGCTATATGTTAGGATTCGAAGGAGCTCCAGGAAAGTCTCATAGCCCCACTGGCCAGAATGGGCTAAATCTCAGAGGGGGAGGGTGGGAGATGGGGGTGACAGTGACCTTTTTTGTGACTCCTCCTAGACCATCCATCCCTGCTCCCAGGAGGACCTGTCCTCCCAGATGGTGGAGATGGACAGGAGGACTATCTACCCACCCGTCCCCACGGCCCTGACCCTCTGACCTCACCCTCTCCGCTGATTTCTTCATGTTAGTTCAACATTACCCAGAGGGGTCAGGACAGACAATTCCTCAGTGACCCAGGAGCTGACACACTATGGCGCACGTCCGAGGCTTGCAGCTGCCTGGCTGCCTGGCCCTGGCTGCCCTGTGTAGCCTTGTGCACAGCCAGCATGGTAAGGGAGTGCTTGCAGGCTGGAACAGGCTGGAGGACTGGGGTGTGGGCCCATGGGCTGGGGTCTCCTGGCTGGACAGAGCACACAGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGG = SEQUENCE_ID=F2_X2 SEQUENCE_TARGET=298,165 SEQUENCE_TEMPLATE=AGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGGAGGGTGGGCTTGCTTCATGCCCCCAGATGGCCAAGACTGCCTGTTCCTGAGGTCGCTGTTCCATGACCCCCCCACCGCCTTTACAGTGTTCCTGGCTCCTCAGCAAGCACGGTCGCTGCTCCAGCGGGTCCGGCGAGCCAACACCTTCTTGGAGGAGGTGCGCAAGGGCAACCTAGAGCGAGAGTGCGTGGAGGAGACGTGCAGCTACGAGGAGGCCTTCGAGGCTCTGGAGTCCTCCACGGCTACGGTGAGCCTGGGCTGCTCGGACGGTGCCGGGGCCTCAGACCGGGCCCAACTCTAGACACTTCCACAGAGAAGCAAGCGAGGAACGCCACAGCCCCTTCGCTGCTCACAGCCTCATTTCAACTCTGAGCCCCTCCTCACAGGGCTGGCAAGAGGAGCGGCCTCAGCCTTTCCTGGGGGTCTCTGTGCCTGGACTGTGTCCCTGTGCAGCTCCATGACATGGGGAGGCCTCCACAGTCTTCAGACATCCACCTGCCTTGGAGCTCTGTGTCCACATGGCCTCCTCAGCGGCAGACTCCCACAC = SEQUENCE_ID=F2_X3 SEQUENCE_TARGET=298,29 SEQUENCE_TEMPLATE=TGTTCCTGGAGCTCTGTGTCGCCTTTCCTGTCTGTAGGGACTCTGCCAGGGACCCACTGCCCCCTCTCCTCCCATCTCCCCCAGCCTCTTTCAGACTCGGTGTGTGTGTTGGAGGAACTCCCCTATCCTCAAATATTCTTCTCCTTTTGGAAACAAAAGTAGGAAACTCTGCCACAAACCTCCCCAGAGCCTGCCCCCTGCGTGACCAGGGTAAGGAAAGTGTGAGGAGGAGCATAACATTTACTAAAACAACACAAAACAGGAGCTGCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCA = SEQUENCE_ID=F2_X4 SEQUENCE_TARGET=298,55 SEQUENCE_TEMPLATE=GCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCAACTGACACGGGTTTGGGGAGCAGGACATGGAGGGGAGCTTGGGAGAAGAGCTCAGGGGTGGGTTTGGAGTGTGGCTGGTGGAGGCCGAGGCAGTCCCCAGCATCTGACATTGCTCCCATTCCTGGGGTCAAGATGTCTCTTTGTACCTGGCTCTGTGTCTGGCATGCGAACGAATGAATGAATGAATGGACTAATGAATTAATGTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCACTGTAAACTCCGCCTCCCGGA = SEQUENCE_ID=F2_X5 SEQUENCE_TARGET=298,110 SEQUENCE_TEMPLATE=TGCTGGCCAAGAGAAGCCACAGAATGGAAGCTCCATGAGGGCAGGGCTGTGACTGTCCTATTGGTTGATGTGTACTGAGCACCCGACAGTGCCTGTCATATGGTAGGCACTTAGCGAATATTTGGAGGCCACTGTTGAGTGAATGGGAGAACTGCTGGTTGCAGAGGAAGAGGGGCTGGGTGAATGCAGGTTCAGGATTGTGGACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGG = SEQUENCE_ID=F2_X6 SEQUENCE_TARGET=298,141 SEQUENCE_TEMPLATE=GACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGGATGGGAACCAAGAATACTGGCTACCCAGGCACAGTGGCTCATGCCCGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAGGTCAGGGGTTTGAGACCAGCTGGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAATTGCCAGGCGTGGTGGTGGGCGCCTGTAATCCCAACTACTCTGGAGGCTGAGGCACGAGAATCGCTTGAACCCGGGAG = SEQUENCE_ID=F2_X7 SEQUENCE_TARGET=298,319 SEQUENCE_TEMPLATE=GCAGCTCCAGGCAGCCACTACCAGTTGGTTACAGATGGCCTAGGAGGCCAAACCTGGTTACTATCTCTGGTTTATTATGTGCCAGACACTTATGCTGTATATTTTGTTTAATCCTCTCAACAAACCTGCAAAAGTGGCATTAGTAACCCCTTTAAAGGCAAACGGTCAGAAGCCCAGAGAGGTTAAGTAACCTGAGGTCACACAGGCAGAAAGCAGCAAGACCGGGGTTCACACCCCTGTCTGTTCCGGTCCATGTGTGGTCTCACTCACTCTGCTGCCTCCTTGCCCCTCACCCACCAGGCCAGGATCAAGTCACTGTAGCGATGACTCCACGCTCCGAAGGCTCCAGTGTGAATCTGTCACCTCCATTGGAGCAGTGTGTCCCTGATCGGGGGCAGCAGTACCAGGGGCGCCTGGCGGTGACCACACATGGGCTCCCCTGCCTGGCCTGGGCCAGCGCACAGGCCAAGGCCCTGAGCAAGCACCAGGACTTCAACTCAGCTGTGCAGCTGGTGGAGAACTTCTGCCGCAACCCAGACGGGGATGAGGAGGGCGTGTGGTGCTATGTGGCCGGGAAGCCTGGCGACTTTGGGTACTGCGACCTCAACTATTGTGGTGAGCTGCCTGGGTAGGGGGCCTGAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCC = SEQUENCE_ID=F2_X8 SEQUENCE_TARGET=298,133 SEQUENCE_TEMPLATE=GAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGG = SEQUENCE_ID=F2_X9 SEQUENCE_TARGET=298,131 SEQUENCE_TEMPLATE=CCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGGCTTTACAGATGACAACAGCTGAGCATCCAGGATCCCACCAACTCCACACAGCAGCCACATGAGATGGGTTGTTTACTTCTTTTTTTTTTGTTTCTTAGATGGAGTCTTGCTCTGTCACCTAGGCTGGAGTGCAGTGCTGCAATCTCGGCTCACTACCTCGATCTCAGCTCACTGCAACTTCTGCCTTCCGGGTTCAAACGATTCTCTTGCC = SEQUENCE_ID=F2_X10 SEQUENCE_TARGET=298,172 SEQUENCE_TEMPLATE=CCTGAGCCCAAGAGGCTGAGGCTTCAGTAAGCTGTGACTGTACCATTGCACTCCAGCCTGGGTGACAAGAGTGAGACCCTGTCTCAAAAATAAATAAATAAATAAATAAAAAGTGTGAGGCAGCCCCTCAGCATCACACGGAGGCTCCAGCCCCAAAGGCGGCCAGCCCAAGCTTGGATCTGGGCCCCGGAGGCAGCTCTGCCCAGCTGGGTTCTTAGACCTGGGATTGTTACTTCTAGGGCTGGTGTAGAGGCAGCCCCCTCATCCTCAGCTCCTAATGCTTCCTGCTGCCCCTCCCAGGCAGGTGATGCTTTTCCGGAAGAGTCCCCAGGAGCTGCTGTGTGGGGCCAGCCTCATCAGTGACCGCTGGGTCCTCACCGCCGCCCACTGCCTCCTGTACCCGCCCTGGGACAAGAACTTCACCGAGAATGACCTTCTGGTGCGCATTGGCAAGCACTCCCGCACAAGGTACAGAACTGGTGGCCCGTGGGTGTCTGGCAGGGGTCTGAGTCCTCCAAAGCGATCATGAGGGGCCTTGGTGGCTCCGGGACACATAGGATGTTCTGTATACCCCCCAGAATATAACATCCCAGCAGTCTCTGCTGGAAAGCCATTTGGTCACGTCCTGACTGAGGCTTGGAGCGCGGGGAGAATCCGTCTGTCTCTGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGC = SEQUENCE_ID=F2_X11 SEQUENCE_TARGET=298,178 SEQUENCE_TEMPLATE=TGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGCTGTCTGACTCCAAAGCCCTGCACGGCTTTAGGCCCAGGAAGAAACACCCAGGGGGCTGCCATGGCAGGAACCAGCCCTATCCCCTCCCTGGTGGCCTGCAGGACACACTGTCTCCCAGAACCCCAAGGGCAGGCAGTTTCCTGCTCCTTGCTGGGTGAACCTGCAGCTTCTCCATTTCTTTCTTGGGGTCTCTGCAGGTACGAGCGAAACATTGAAAAGATATCCATGTTGGAAAAGATCTACATCCACCCCAGGTACAACTGGCGGGAGAACCTGGACCGGGACATTGCCCTGATGAAGCTGAAGAAGCCTGTTGCCTTCAGTGACTACATTCACCCTGTGTGTCTGCCCGACAGGGAGACGGCAGCCAGGTGGGCCACCAGATGCTTGTTAGCTGAGGGGCAGAAGCCAAGTTCTGGGCCTGGCTCTGATACCAAGTAGCCTTGCAAGAGCCCCTTTCCCTTTTCCAGGCCTCGGTTTCTTGGAGTGAACCCAAAAGTTCTTTTCAGTACTGGCGTTTTATTTTTTATTTATATTTATTTATTTACTGACGGAGTTCCACTCTTGTCTCCCAGGCTGGAGTGTAGTTGTGCGATCTTGGCTCACTGCAACCCCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACA = SEQUENCE_ID=F2_X12 SEQUENCE_TARGET=298,186 SEQUENCE_TEMPLATE=CCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACAGGCTAATTTTTGTATTTTTAGTAGAGACTGGTGGGTTTCACCGTGTCGGCCAGGTTGGTCTCGAACCCCTGACCTCAAGTGATTCACCCGCCTCGGCCTCCCAAAGTGCCGAGACCACAGGCGTGAACGTCTGTGCCCAGCCAGCTCTGGCGTTTTAGATTCTGGTCTCTAAGAAATGGCGTTGGGGCCAGGCGGCTCCTGTGGGGGTTGGCTCTCACTAGGCCCTTCTTCCTTCCCCAAAGCTTGCTCCAGGCTGGATACAAGGGGCGGGTGACAGGCTGGGGCAACCTGAAGGAGACGTGGACAGCCAACGTTGGTAAGGGGCAGCCCAGTGTCCTGCAGGTGGTGAACCTGCCCATTGTGGAGCGGCCGGTCTGCAAGGACTCCACCCGGATCCGCATCACTGACAACATGTTCTGTGCTGGCAAGTCTGTGCAGGGCGGGCTGAGGGAACAGTGGGGCCCAAGCTGGGAGAACTGAGTTGTGCCTGGGTTCAAGCCATGTGACTTTGAGCAAGTTGCCTAACCTCTTGGTGGCTCAGTTTCTTCCTCTGTAAAATGGAGGTAAAAGTCTCTATCCCATAAGGTTATGGGAGGGTTAAATGAAGTAGTATATATTAATGTACTTGGCATAGTATCAGTCACCAGTGAGCTCAGATAGCAGCAAGAGGCTGCGGGTAGGGAAATGCCATTCATTCAGTCACTCAGCAAATATTTATTGAGCG = SEQUENCE_ID=F2_X13 SEQUENCE_TARGET=298,75 SEQUENCE_TEMPLATE=AACTTTTTAAAAGCATTATTCTGCCTGTTGGGTGGAGAATAGACTGTAGGTGGGCAAAGAATGAAGGAAACTAGTGGGTTCAGGAGCTCGAGCTAGAAGTGGTGAGAAGGGTTTGGATTTGGGGTCTATGCTGAAGGTAGAGCCGACAAGATTTGCTAGGATTGGATGTGTAGGGTGAGGAAGTGGGGACAGCAAGAATGACTGGAGGGGTAAGTGGACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTC = SEQUENCE_ID=F2_X14 SEQUENCE_TARGET=298,148 SEQUENCE_TEMPLATE=ACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTCATATTCTGGGCTCCTGGAACCAATCCCGTGAAAGAATTATTTTTGTGTTTCTAAAACTATGGTTCCCAATAAAAGTGACTCTCAGCGAGCCTCAATGCTCCCAGTGCTATTCATGGGCAGCTCTCTGGGCTCAGGAAGAGCCAGTAATACTACTGGATAAAGAAGACTTAAGAATCCACCACCTGGTGCACGCTGGTAGTCCGAGCACTCGGGAGGCTGAGGTGGGAGGAT = SEQUENCE_ID=F2R_X1 SEQUENCE_TARGET=235,90 SEQUENCE_TEMPLATE=CTTCCTCGCTCGCGCCCGCGCGACCGCGCGCCCCAGTCCCGCCCCGCCCCGCTAACCGCCCCAGACACAGCGCTCGCCGAGGGTCGCTTGGACCCTGATCTTACCCGTGGGCACCCTGCGCTCTGCCTGCCGCGAAGACCGGCTCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCGGAGCAGCCCGAGGCGGGGCAGCCTCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGGGCCGCGGCGGCTGCTGCTGGTGGCCGCCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGCCCGCACCCGGGCCCGCAGGCCAGGTGAGA = SEQUENCE_ID=F2R_X2-seg1 SEQUENCE_TARGET=4,702 SEQUENCE_TEMPLATE=TTTCAGAATCAAAAGCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACCCCAATGATAAATATGAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTGAATACAGATTAGTCTCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCATCTCAGAAGATGCCTCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTGTGTACACCGGAGTGTTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCATCCTGAAAATGAAGGTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGGCCACGGCAGATGTGCTGTTTGTGTCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATTGGCAGTTTGGGTCTGAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGTAACATGTACGCCTCTATCTTGCTCATGACAGTCATAAGCATTGACCGGTTTCTGGCTGTGGTGTATCCCATGCAGTCCCTCTCCTGGCGTACTCTGGGAAGGGCTTCCTTCACTTGTCTGGCCATCTGGGCTTTGGCCATCGCAGGGGTAGTGCCTCTCGTCCTCAAGGAGCAAACCATCCAGGTGCCCGGGCTCAACATCACTACCTGTCATGATGTGCTCAATGAAACCCTGCT = SEQUENCE_ID=F2R_X2-seg2 SEQUENCE_TARGET=0,514 SEQUENCE_TEMPLATE=TGATGTGCTCAATGAAACCCTGCTCGAAGGCTACTATGCCTACTACTTCTCAGCCTTCTCTGCTGTCTTCTTTTTTGTGCCGCTGATCATTTCCACGGTCTGTTATGTGTCTATCATTCGATGTCTTAGCTCTTCCGCAGTTGCCAACCGCAGCAAGAAGTCCCGGGCTTTGTTCCTGTCAGCTGCTGTTTTCTGCATCTTCATCATTTGCTTCGGACCCACAAACGTCCTCCTGATTGCGCATTACTCATTCCTTTCTCACACTTCCACCACAGAGGCTGCCTACTTTGCCTACCTCCTCTGTGTCTGTGTCAGCAGCATAAGCTCGTGCATCGACCCCCTAATTTACTATTACGCTTCCTCTGAGTGCCAGAGGTACGTCTACAGTATCTTATGCTGCAAAGAAAGTTCCGATCCCAGCAGTTATAACAGCAGTGGGCAGTTGATGGCAAGTAAAATGGATACCTGCTCTAGTAACCTGAATAACAGCATATACAAAAAGCTGTTAACTTAGGAAAAGGGACTGCTGGGAGGTTAAAAAGAAAAGTTTATAAAAGTGAATAACCTGAGGATTCTATTAGTCCCCACCCAAACTTTATTGATTCACCTCCTAAAACAACAGATGTACGACTTGCATACCTGCTTTTTATGGGAGCTGTCAAGCATGTATTTTTGTCAATTACCAGAAAGATAACAGGACGAGATGACGGTGTTATTCCAAGGGAATATTGCCAATGCTACAGTAATAAA = SEQUENCE_ID=F3_X1 SEQUENCE_TARGET=298,104 SEQUENCE_TEMPLATE=ACCGGGAGGAGGCGGGGCAGGGGTGTGGACTCGCCGGGGGCCGCCCACCGCGACGGCAAGTGACCCGGGCCGGGGGCGGGGAGTCGGGAGGAGCGGCGGGGGCGGGCGCCGGGGGCGGGCAGAGGCGCGGGAGAGCGCGCCGCCGGCCCTTTATAGCGCGCGGGGCACCGGCTCCCCAAGACTGCGAGCTCCCCGCACCCCCTCGCACTCCCTCTGGCCGGCCCAGGGCGCCTTCAGCCCAACCTCCCCAGCCCCACGGGCGCCACGGAACCCGCTCGATCTCGCCGCCAACTGGTAGACATGGAGACCCCTGCCTGGCCCCGGGTCCCGCGCCCCGAGACCGCCGTCGCTCGGACGCTCCTGCTCGGCTGGGTCTTCGCCCAGGTGGCCGGCGCTTCAGGTGAGTGGCACCAGCCCCTGGAAGCCCGGGGCGCGCCACACGCAGGAGGGAGGCGACAGTCCTGGCTGGCAGCGGGCTCGCCCTGGTTCCCCGGGGCGCCCATGTTGTCCCCCGCGCCTACGGGACTCGGCTGCGCTCACCCAGCCCGGCTTGAATGAACCGAGTCCGTCGGGCGCCGGCGGGAGTTGCAGGGAGGGAGTTGGCGCCCCAGACCCCGCTGCCCCTTCCGCTGGAGAGTTTTGCTCGGGGTGTCCGAGTAATTGGACTGTTGTTGCATAAGCGGACTTTTAGCTCCCGCTT = SEQUENCE_ID=F3_X2 SEQUENCE_TARGET=298,116 SEQUENCE_TEMPLATE=CTCTGGGCTCTTCCCGGTCAAGTTATAACAAGGCTTTGCCCATGAATAATTTCAAACGAAAATGTCAAGATCCTTGCCGGTGTCCTGGGATTACAAGGTGAATCTTGTCATGAAGAAATTCTAGGTCTAGAAAAAATTTGAAGATTCTTTTTCTCTTGATAATTCACTAATGAAGCTTTTGTGGTTGAAAAATAAAAAGTGAGGTTTATGGTGATGTCAGGTGGGAAGGTGTTTTATACATCAATACATTCGAGTGCTCTGAAGTGCATGTAATAATAGCTGTTTCTCTGTTGTTTAAAGGCACTACAAATACTGTGGCAGCATATAATTTAACTTGGAAATCAACTAATTTCAAGACAATTTTGGAGTGGGAACCCAAACCCGTCAATCAAGTCTACACTGTTCAAATAAGGTAAGCTGGGTACAGAAAAAGAAAATTAAGGTCTTTGATGTTTCTACTGTCCTATGCTGAACAAGAATGTCTTTAAAGCTGATTACTGGATGAAATTATTTAACAGATGACGAAGAAGAAGGGATTCTTGGCAATTCGCTGGCCGGTGTCATACTCTATTAGGCCTGCAACATTTCCAGACCTTAAACTGATAGAACATTTTAATTGTTTTAATTGTTTTTGGAAATGATGGGAGAGTTCCTAAGTGGAGTATAAACTGTGGAGAGATGAACCATCTTGAGTAGGCACTGAAGTGTGCTT = SEQUENCE_ID=F3_X3 SEQUENCE_TARGET=298,204 SEQUENCE_TEMPLATE=TGAGGACTCAAACATGCATCCCAAGTGCTCGGTGTTGCTAGGTATGATGGCTCACACCTGTACATTCAGCACTTTGGGAGGCCGAAGCAGAAGGATCAGCCTGGGCAACATAGCAGGACCCCATCTCTACAAAACAATGTTTAAAAAAAAGCAAAGTGCTCAGCACAGTGACTGCATCATTAGGATTGATTGTAGGGCTCCTGATGTTAGCACAGAACACCACAGCCAGGAAGCAGTCTATCTTGTTGGGTGCAAATTGTAACATTCCATTTATGTTTCTTCCTTCTTTTCTTTCTTTAGCACTAAGTCAGGAGATTGGAAAAGCAAATGCTTTTACACAACAGACACAGAGTGTGACCTCACCGACGAGATTGTGAAGGATGTGAAGCAGACGTACTTGGCACGGGTCTTCTCCTACCCGGCAGGGAATGTGGAGAGCACCGGTTCTGCTGGGGAGCCTCTGTATGAGAACTCCCCAGAGTTCACACCTTACCTGGAGAGTAAGTGGCTTGGGCTGTAATACCGTTCATTCTTGTTAGAAACGTCTGAACATTCTCGTGATCTTGTGCCTTTAGGGGCTACAAAATTAAAAATATTTATTCTTTTTTTCTCAGAAACTGGTATGTATCACAGCCCTCTTCACACATTCCAGATGTGGTAGGAGGTTCACAGAATGTGAACTTTTGGAGCTGATGACAGTGTCATCAAGTAACTTTCTCCCCCAGTCTGTCCCCAGACCCTGTTACTGTCCTCAGTAAGCGGCTGAATGTGTGTTGGGAGAGGGCGGGCCAGGGAAGCGG = SEQUENCE_ID=F3_X4 SEQUENCE_TARGET=298,183 SEQUENCE_TEMPLATE=TGGTTAAATAAAATATATTATTAAAATTAATTTCACCTGTTCCTTTTTACTTTTTCTAATGTGACTACTAGAAAACTTAAAATGACATCTGAGGCTCCATTGTCTTCCCCTTGGGCCAGCACTACCACAGAATGTCTTAGGATTCAGCTCCAGGCCGCCACGCCTGCTTCTTTCAGGGAGCTGGTTCTATGCACATGTTTTATATGAGAGATAATTAAGTTGTCAATTGTGATAACAAAACAGGATTTGACTTTGTACAGAATTCTTTGGTTCCAACCAAGCTCATTTCCTTTGTTTCAGCAAACCTCGGACAGCCAACAATTCAGAGTTTTGAACAGGTGGGAACAAAAGTGAATGTGACCGTAGAAGATGAACGGACTTTAGTCAGAAGGAACAACACTTTCCTAAGCCTCCGGGATGTTTTTGGCAAGGACTTAATTTATACACTTTATTATTGGAAATCTTCAAGTTCAGGAAAGGTGAGCATTTTTTAATTTGTTTTTATGACCTGTTTTAAATTGTGAATACTTGGTTTTACAACCCATTTCTTCCCCAATTCAAAAATAGCAGAACAGAGTTGTTGAGAAGGTGATGGAGTAGAAGGGGGAGCGCGCACTGTGGGGAGGGGTGGACAACAGGCCTGGTCCTACCTGTGACTCTGCACTACCCTGTGACTCTGGCAGGGCCCCCTCGGAGACCCAGGTTCCTCAGCCAACCGGCTGGATCAGGTCATCTCTAAAGGTCCCGCCACGCTCACATTTCTCCCTCTATTGAGGATC = SEQUENCE_ID=F3_X5 SEQUENCE_TARGET=298,164 SEQUENCE_TEMPLATE=CAAAATTTGTTTTTGGTTCAATGCATAATACTCCCTTCCTTTTTCTTTTACTGCAGATATCTTCTAAAGGGGCTCAATAGGGTTCAATATGCCTAAATTGGATCTTCTCAGTCTTGGAAAAGGCATTTTTAGCAGTGATCAAGGGAAACTGATTAGCGAAGTCACTTCTAATCCTTCACGTGTCAGCTGTGTTCTTGTAGGCTTTGCTTAGAACCTAGGTTTTTACTTCCACAGTGACTTAATAAAGGGGAAAGAATTGACTCAGAGCCCAGATGAATTAAGAACTCTATCTTTTTACAGAAAACAGCCAAAACAAACACTAATGAGTTTTTGATTGATGTGGATAAAGGAGAAAACTACTGTTTCAGTGTTCAAGCAGTGATTCCCTCCCGAACAGTTAACCGGAAGAGTACAGACAGCCCGGTAGAGTGTATGGGCCAGGAGAAAGGGGAATTCAGAGGTGAGTGGCTCTGCCAGCCATTTGCCTGGGGGTATGGGTGCTGTGGGTGACTTCTGGAGGAGTAGCTCCACCCTCAGGGCTGGGATATACTTCCTTGGTTAAATATTCAGGAAAACAAACTGCCTGGAGGTTTTTTGTTGTTATTTGTTTGTTTTGGTTTTGATTTTGCTTTGGTACAAAAAAGATTTTGGACATTTAGAAATGTTTCTGTGTTGATTGTGCCCTTGTATTAGCAGGTGTTTTCTTGAGCACCTGTCATGTGCTAAGCCCTCTGCTGAGCACTGGATACACAAACTGTGT = SEQUENCE_ID=F3_X6 SEQUENCE_TARGET=298,141 SEQUENCE_TEMPLATE=CAGCATCGTTTTAGGCACTTCTTTCCAGCCATTGTTCTTACCTCCAACTACATATCTTTTCTGGACTGTGCATTATTCAGTTTATTAAATGCCCATTAAATGTGTTTAGCCATTGTCAATTACTCTGAAACGTTCAGGTTTTGACAAATTCTTTCCTAATGTAAGTGTGGTGGAAAGAGTGAAAGAAAGTCAAATTGCACAAAAATAGGATGGTGTAATTTGGGGTTATGCCGTCAATTTTGTCCACTGATAAATGGGATTTGAGCTCTCCAAGTTGACTAGATGCCCTTTATTTTTCAGAAATATTCTACATCATTGGAGCTGTGGTATTTGTGGTCATCATCCTTGTCATCATCCTGGCTATATCTCTACACAAGTGTAGAAAGGCAGGAGTGGGGCAGAGCTGGAAGGAGAACTCCCCACTGAATGTTTCATAAAGGAAGCACTGTTGGAGCTACTGCAAATGCTATATTGCACTGTGACCGAGAACTTTTAAGAGGATAGAATACATGGAAACGCAAATGAGTATTTCGGAGCATGAAGACCCTGGAGTTCAAAAAACTCTTGATATGACCTGTTATTACCATTAGCATTCTGGTTTTGACATCAGCATTAGTCACTTTGAAATGTAACGAATGGTACTACAACCAATTCCAAGTTTTAATTTTTAACACCATGGCACCTTTTGCACATAACATGCTTTAGATTATATATTCCGCACTCAAGGAGTAACCAGG = SEQUENCE_ID=F5_X10 SEQUENCE_TARGET=59,219 SEQUENCE_TEMPLATE=TTATTTATTATCATGAAATAACTTTGCAAATGAAAACAATTTTGAATATATTTTCTTTCAGGCAGGAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTATAAGTGGAACATCTTAGAGTTTGATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATACTACAGTGACGTGGACATCATGAGAGACATCGCCTCTGGGCTAATAGGACTACTTCTAATCTGTAAGAGCAGATCCCTGGACAGGCGAGGAATACAGGTATTTTGTCCTTGAAGTAACCTTTCAGAAATTCTGAGAATTT = SEQUENCE_ID=F7_X1 SEQUENCE_TARGET=298,68 SEQUENCE_TEMPLATE=ATTTGAAGTGTTGGTGCCACACACACCTTAACACCTGCACGCTGGCAACAAAACCGTCCGCTCTGCAGCACAGCTGGGGTCACCTGACCTTTCTCCTGTCCCCCCCACTTGAGCTCAGTGGCTGGGCAGCAGGGGATGCATGGCCACTGGCGGCCAGGTGCAGCTCTCAGCTGGGGTGTTCAGAGGACGCCTGTGTCCTCCCCTCCCCCATCCCTCTGTCACCCTTGGAGGCAGAGAACTTTGCCCGTCAGTCCCATGGGGAATGTCAACAGGCAGGGGCAGCACTGCAGAGATTTCATCATGGTCTCCCAGGCCCTCAGGCTCCTCTGCCTTCTGCTTGGGCTTCAGGGCTGCCTGGCTGCAGGTGCGTCCGGGGAGGTTTTCTCCATAAACTTGGTGGAAGGGCAGTGGGCAAATCCAGGAGCCAGCCCGGGCTTCCCAAACCCCGCCCTTGCTCCGGACACCCCCATCCACCAGGAGGGTTTTCTGGCGGCTCCTGTTCAATTTCTTTCCTTCTAGAAACCAGCATCCAGGCACAGGAGGGGAGGCCCTTCTTGGTAGCCCAGGCTTTGGCGGGATTATTTTTCAAAGAACTTTAGGAGTGGGTGGTGCTTTCCTGGCCCCCATGGCCCTGCCTGTGAGGTCGGACAAGCGCAGGGAGTCT = SEQUENCE_ID=F7_X2 SEQUENCE_TARGET=298,70 SEQUENCE_TEMPLATE=TGTGTGGGGTGTGGGGATAGGGTCAGGGGATGGCGTGGGGGGTGTGGCATGGGGATGGCACGTGTGGCATGGGGATGGGGATGGGGGGTGGGGCATGGCCGAGTGGGGCTGGGGCTGGGAATGGTGAGTGGGGCATGGGGATGGCGAGTAGGGGGTGTGGCGTGAGGATGGCTAGTGGGGCGTGGGGATGGCGTGTGGGGATGGCGAGTGGGGGGTGGGCTGTGAGGGACAGTGCCTGGGATGTGGCTGCAGCCCTAGCTCACAGCATGGCCTTATGACCCCGGCCACCTTCCTGCCCAGGCGGGGTCGCTAAGGCCTCAGGAGGAGAAACACGGGACATGCCGTGGAAGCCGGGGCCTCACAGAGGTGAGCAGGGACTGCCACTGGTTTTGTCCTGGGGCCCAGTGGGGGCAACATCACCTCCTTCCCCTCCCATGGCAAAGAGCCAGCCCGCGGGGTGGCTACTGCAGTGCCCCCCAAGGAGGGTGTTCCCTGCTCGAGAGGAAGTGACCGCTCCAGCTTGGCCTTCCCTGGGACTGGGGTGCAGGCGATTTTATCTTCTTTGCTCCATTCTGTTCCTTCCAGATAATCGTGTGTTCTTCATCAGGTTTTCCTCAGTTCTTGAGAGCTTTTCTGATGCAAATCTGCTTTCACCCCAGGGCGGTC = SEQUENCE_ID=F7_X2' SEQUENCE_TARGET=298,165 SEQUENCE_TEMPLATE=AGCCTCACGTTTACCGCGGCGCCCGCAGCCCCCTTCGCCCGCTTCCGCGCGTGCCCCCGAGCGCGCCCTCGGGATCAGCCCCCGGAAGCAGAGAGGCCAGGCCGGGAAGGATGGGCGAACGGGGTGGCTGACCCGGGAGCACGGCAGGGAGGACACCCAGCCAGGCCCGCGAGCAGCGCCGCTCCCCTCCTCCAGGACGGGCGGGAACCTGCGATGCCCCCGCCGCGTGGGCCGTGGGGCGGTCTCCGAGGCACTGGGCGGGGCACGCGGTGGGCGCTTCACGGAACTCGCATTTCCCAGTCTTCGTAACCCAGGAGGAAGCCCACGGCGTCCTGCACCGGCGCCGGCGCGCCAACGCGTTCCTGGAGGAGCTGCGGCCGGGCTCCCTGGAGAGGGAGTGCAAGGAGGAGCAGTGCTCCTTCGAGGAGGCCCGGGAGATCTTCAAGGACGCGGAGAGGACGGTGAGCCCAGCCTCGGGGCGCCCCGCGCGGACACTGCACGGCGGCGGTGAACCAGGCCGCGTGGGGCCGCCTGCGTCTCTTTGGCTGCGGCCTGTGGGCGGCGAACACGCAGCGGCGCCCGCGCGCGCGCTCTCTCTGCGGGGGTCGCTTTCCGCCCGGGGTGACTCCGCTTTCCTGGGCGATGCCCCCACCCCCAGGCACGCGCTCTCCCCGTGCGGCCGCACCGCGCATGCCGGTTTTCACATCAGAAAATACGATTTGCACAAGCACACTTAGGGTGTCCCCCTTAACTTCCCAAGG = SEQUENCE_ID=F7_X3 SEQUENCE_TARGET=298,29 SEQUENCE_TEMPLATE=CAGAGCCTGTGTACCTCGTCCCAGGTCCACAGCTCAGCGACAGAAGAGTCAGGGTTGAACCTCGGGTGTTCTGACTTGGGAGCAGGAAATGTGTGGTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATT = SEQUENCE_ID=F7_X4 SEQUENCE_TARGET=298,118 SEQUENCE_TEMPLATE=GTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATTCAGGGCTCAGCCCAGTTGGAAATTGGTCTAGGTGACCTTTAAATCCCTTCCAGTCTGAGGTCTTTGACAGGGACCCAAGGTTCTGATTATCAGACTCAGTGGCCCCCTTCGCGGTCCCGGCCCTGGGCAACTTCTCAGCCCTGGAGACTGGCCCAGTTGAGAGTCCCTGTGTCCCGTGTGCCCA = SEQUENCE_ID=F7_X5 SEQUENCE_TARGET=298,145 SEQUENCE_TEMPLATE=GCTCGGAAGAGTGGTCACCCTGGAGGCCACTGCCCACCAGCCAGGCACCCCCCAAATGCAACCGCAGCCAGCACTGCCAGCCACTGGCAAGGCTGTTCAGACATGTGGCTCCTCTGATCCACGCCTTGTCCTTTGGATCAGTCCACGGAGCAGTGTGCCAAGCTCAGGCTCTGTCACCCACAGCTCATGCCACCTTCCAGGCAGAACACCACTGCTGACCCAGGGGCATGGCCACCCCGGGGGCTGGCGTCTCGCTGACCCCCAGAAGCCCCTCTCAGGGTGTCCCCTTCCTGTCCCCAGACAAGGATGACCAGCTGATCTGTGTGAACGAGAACGGCGGCTGTGAGCAGTACTGCAGTGACCACACGGGCACCAAGCGCTCCTGTCGGTGCCACGAGGGGTACTCTCTGCTGGCAGACGGGGTGTCCTGCACACCCACAGGTGACCAGGCTTCATGTCCCAGTCCCAGATGACACCAGTCCCTGTCCCACTAGGATTATCTTACTGGACAAAAGACGGGTGGGACTGGCCTTCACATCTACTGAGCACTAACTATGCACTGACCAATTGTGAGGTGGGATCTGGGCACCAAGGGTGGCACAGGCCAGCAGCGACCAGTGACTAGGATGGGCACCCTGGGGGCAATCCCTGAATGGCCTCAGGCCCCCTGCCAACTTCTAGGCAGACCAGGGGAGCCAAGCAAGGCACTATCTCACGTCCAACTGCCCACTCGCAGGAATC = SEQUENCE_ID=F7_X6 SEQUENCE_TARGET=298,114 SEQUENCE_TEMPLATE=GAAAAGTGAGCAGAGGGCGGTTTTAAGACGTAAGCCCTCTGTTTCCTCCAAAACCAGCCCTGACCATTGTCTCCTCAGCCAGCCACTTCTTCAAGGGCCTCTCATGGCCGGGCCCCACCAGTCAGGCCCAGCCGAGGCCCTGCCTTCCACCACCCCTGGGCCCTGGGAGCTCCTGCTCCTGGGGGCCTCCCATAGCCTCGGCCTCAAGGCCTCTCAGAGGATGGGTGTTTCTGAATCTTTCCTAGTGGCACGTTCATCCCTCACAAATCTCTGCATCTTTCTGACTTTTGTTTTACACAGTTGAATATCCATGTGGAAAAATACCTATTCTAGAAAAAAGAAATGCCAGCAAACCCCAAGGCCGAATTGTGGGGGGCAAGGTGTGCCCCAAAGGGGAGTGTCCATGGCAGGTAAGGCTTCCCCTGGCTTCAGGATTCCAAGCCCTGAGGGTCTTGAAGCCTTTTGAATGTGAACAACAGCTCTGGAAGGGAAAATGGGCAGGTCAGCCCAAGCCCACAGGCTCCAAGTCAGCACACCTAGCACCTCCAGCTCGCGGCACCCCCATGCTTTTAGTGGGGCAAGGAAGGAGAAAAGAAAACGACACTCACTGAGGGTCTACCCTGTGCAGAGAACCCTGCGAGATGCCCCATCCGAGTTGTCACGTCGTCCTCACGGTTACTCTTTGAGGTGGGATCTTTGCCTGATCTTTG = SEQUENCE_ID=F7_X7 SEQUENCE_TARGET=298,128 SEQUENCE_TEMPLATE=CTTTGCAAAATCAGGAGCATTGGATCAAAGCTATGTGAAGATCCTGTGAGGTGAACAGTGAAATCTCACAGCGACATTTGTATTCTTGGGCCGTGCCCAAGAGCACGTCTCGGCTAGAGAGGGGCACAGCCTCCCAGAGCCAGGTCTGAGCAGCTTTGCCTGGGAGGGATCTGCAAAGACCCCAGGATTTCAGAAAGAAATTGTGCAATGCCAGAGGTTCCTTGGCATGCCCGGGAGGGCGAGTCATCAGAGAAACAATGACAGCAATGTGACTTCCACACCTCCTGTCCCCCCGCCCAGGTCCTGTTGTTGGTGAATGGAGCTCAGTTGTGTGGGGGGACCCTGATCAACACCATCTGGGTGGTCTCCGCGGCCCACTGTTTCGACAAAATCAAGAACTGGAGGAACCTGATCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTTCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCGCTGTCCGACCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCTGACCGCAGCTCTCAAGTGTCTCAGGGGCTGTGGCTCTGGGCTTCGTGCTGTCACTTCCACAGACAGACAGACATCCCCAAAAGGGGAGCAAC = SEQUENCE_ID=F7_X8 SEQUENCE_TARGET=298,600 SEQUENCE_TEMPLATE=GCCGGGAAGAACTGTGGAGAATCGGGGCACGCCCTGTCCTCCCAGCTGCCAGGGCACAGCATCCCTTCCCCACCTGCAACACCCAGACCCCAGATTCACCCCAGTTCACTTGTCCCCACACGAGCCACAGGCTGCCACCTGGGGCAGGCTGGCCCACCTTGGGGTTAGATGCAGGTCCCCTTGCCCCAGAAGGAGACTGCAGCCCCTGCAGACCTAGAAATGGCCACAGCCCATCCCCATGCACCAGGGGGTGAGGTGGCAGGTGGTGGAAAGGGCCTGAGGGGGGCTTCTTCCTTCCAGGCGAGCACGACCTCAGCGAGCACGACGGGGATGAGCAGAGCCGGCGGGTGGCGCAGGTCATCATCCCCAGCACGTACGTCCCGGGCACCACCAACCACGACATCGCGCTGCTCCGCCTGCACCAGCCCGTGGTCCTCACTGACCATGTGGTGCCCCTCTGCCTGCCCGAACGGACGTTCTCTGAGAGGACGCTGGCCTTCGTGCGCTTCTCATTGGTCAGCGGCTGGGGCCAGCTGCTGGACCGTGGCGCCACGGCCCTGGAGCTCATGGTCCTCAACGTGCCCCGGCTGATGACCCAGGACTGCCTGCAGCAGTCACGGAAGGTGGGAGACTCCCCAAATATCACGGAGTACATGTTCTGTGCCGGCTACTCGGATGGCAGCAAGGACTCCTGCAAGGGGGACAGTGGAGGCCCACATGCCACCCACTACCGGGGCACGTGGTACCTGACGGGCATCGTCAGCTGGGGCCAGGGCTGCGCAACCGTGGGCCACTTTGGGGTGTACACCAGGGTCTCCCAGTACATCGAGTGGCTGCAAAAGCTCATGCGCTCAGAGCCACGCCCAGGAGTCCTCCTGCGAGCCCCATTTCCCTAGCCCAGCAGCCCTGGCCTGTGGAGAGAAAGCCAAGGCTGCGTCGAACTGTCCTGGCACCAAATCCCATATATTCTTCTGCAGTTAATGGGGTAGAGGAGGGCATGGGAGGGAGGGAGAGGTGGGGAGGGAGACAGAGACAGAAACAGAGAGAGACAGAGACAGAGAGAGACTGAGGGAGAGACTCTGAGGACATGGAGAGAGACTCAAAGAGACTCCAAGATTCAAAGAGACTAATAGAGACACAGAGATGGAATAGAAAAGATGAGAGGCAGAGGCAGACAGGCGCTGGACAGAGGGGCA = SEQUENCE_ID=HCF2_X2 SEQUENCE_TARGET=298,909 SEQUENCE_TEMPLATE=CTCCTCCCTGGGATGCCTGAGCTCCCAGGATTCTAGAAGGTTAGTTTTGCAAACCTTTAAAGAAGGGATTTTCATCAAGGGGCCCACAGATCCTTCATTGAGGTTTATGAGTCCCACATCAAAGGTTGGGTGTCTATCTACATCAGATTCTCTTAAAGTCCATGATCCTAAAACAGTTAAGAACTAATGCTGTGAGGGCCTCTTCCTGGGTCAAAGCCACAGGGAACCTGCCATGTGGATGCTGCAGCGGGGTGTGGATCAGCCAGGCCGCCTTTCACTGTGTTCTGTTTTCCCTCCCAGCTTTAGCTCCGCCAAAATGAAACACTCATTAAACGCACTTCTCATTTTCCTCATCATAACATCTGCGTGGGGTGGGAGCAAAGGCCCGCTGGATCAGCTAGAGAAAGGAGGGGAAACTGCTCAGTCTGCAGATCCCCAGTGGGAGCAGTTAAATAACAAAAACCTGAGCATGCCTCTTCTCCCTGCCGACTTCCACAAGGAAAACACCGTCACCAACGACTGGATTCCAGAGGGGGAGGAGGACGACGACTATCTGGACCTGGAGAAGATATTCAGTGAAGACGACGACTACATCGACATCGTCGACAGTCTGTCAGTTTCCCCGACAGACTCTGATGTGAGTGCTGGGAACATCCTCCAGCTTTTTCATGGCAAGAGCCGGATCCAGCGTCTTAACATCCTCAACGCCAAGTTCGCTTTCAACCTCTACCGAGTGCTGAAAGACCAGGTCAACACTTTCGATAACATCTTCATAGCACCCGTTGGCATTTCTACTGCGATGGGTATGATTTCCTTAGGTCTGAAGGGAGAGACCCATGAACAAGTGCACTCGATTTTGCATTTTAAAGACTTTGTTAATGCCAGCAGCAAGTATGAAATCACGACCATTCATAATCTCTTCCGTAAGCTGACTCATCGCCTCTTCAGGAGGAATTTTGGGTACACACTGCGGTCAGTCAATGACCTTTATATCCAGAAGCAGTTTCCAATCCTGCTTGACTTCAAAACTAAAGTAAGAGAGTATTACTTTGCTGAGGCCCAGATAGCTGACTTCTCAGACCCTGCCTTCATATCAAAAACCAACAACCACATCATGAAGCTCACCAAGGGCCTCATAAAAGATGCTCTGGAGAATATAGACCCTGCTACCCAGATGATGATTCTCAACTGCATCTACTTCAAAGGTAAGAGGCACCTTTACAGTTCTCACAGCAAACCCACAACATACTATTTTTGTATGTGGGTAGATTGAATGCCAAGAACTGTACTGTAGCTATAATTTATCCAGGAAAACTAGACACAAGATTGACTCTGGAACGGGGACAGGGAAGGCCAAGCTGAAGTGACAGTAGCATCTGACACTTACTGAGCCCTAACTCTGTGCTTTAACACAGCCTTGTGAGGTCATCACTGTTATTAGCATCCCCATTTTACAGAGGAAGCCACCAACACATGAAGTAAAAGGATGGGCTGGGCGCGGTGGC = SEQUENCE_ID=HCF2_X3 SEQUENCE_TARGET=298,278 SEQUENCE_TEMPLATE=TTTGACAGATGTGACAGATGAAGAAATAGAAGCGAGTTAGGTGCCTTACCATGGTCAAACAACTAGTTCGTATCAGACCCTACTCCAGAAACTATTCCAGTCCGGGTAACCTCTCGTTAACCTCTCTTGTTAGAAATGCAAATTTCTGCCCAAATCAGGCCTCAGGAATCAAGAGACTGTGGGGTCGGCTCTGCAGGCTATCTGAATGAGGCCTCCAGGGAAATCAGATTCACTCTCAAGGGTGAGACGATTTCCCTAAAGGAACCTTCTCATAACAGCCTCTTCCTGTGGCCTTTACAGGATCCTGGGTGAATAAATTCCCAGTGGAAATGACACACAACCACAACTTCCGGCTGAATGAGAGAGAGGTAGTTAAGGTTTCCATGATGCAGACCAAGGGGAACTTCCTCGCAGCAAATGACCAGGAGCTGGACTGCGACATCCTCCAGCTGGAATACGTGGGGGGCATCAGCATGCTAATTGTGGTCCCACACAAGATGTCTGGGATGAAGACCCTCGAAGCGCAACTGACACCCCGGGTGGTGGAGAGATGGCAAAAAAGCATGACAAACAGGTATTTCACACTGTGTGTTTGTTCTTTTGAGCTCCCAGATGCTGGGGGTGTCTGGGAATACTGGAAAATGGATCATTTTTTTAAAAAGGGAGAATTATGTACAAGTACCCAAGAACTTCCATACAGGGCCACTCTGTTAATTCAGCCCCAATTTGTTGCTTGAGATAAGAGATGATTAGAGAGCATTCATAAGGGACACATCTGCCCTCTAGGGGCCAGTTTCAGAAGTTAGAGGCAGATGACTTAGAGACAGCTTGGTGCTTGCTTTGTGGCTTCGAGTCCCAGCTTCATCATCCCTAA = SEQUENCE_ID=HCF2_X4 SEQUENCE_TARGET=298,149 SEQUENCE_TEMPLATE=ATGCCTTTTTAAACCATAAACCAGGAAAAGTTCTGCTGCTAACCTTGAAGATAGGAAACGAACCATACAGTCTCAAGGAAATAATCATGCAACAGAAAACACACCTCAGTTTTCAGTAGCGGAATTACAAAGGAGTGTGCTTCCTAAAATCCTCAACTGACAGTCCCGGAATATAAATTTTAATAAGTGCTATATCAATTCTGTGATAAATATAACCCGTGGCCCTTTAAAGGGAAAATCATGATTCTTTTGTAACTTGTGGTTCAATAAAACTGGGCCCCCCTTTCCTTTTCTGTCTAGAACTCGAGAAGTGCTTCTGCCGAAATTCAAGCTGGAGAAGAACTACAATCTAGTGGAGTCCCTGAAGTTGATGGGGATCAGGATGCTGTTTGACAAAAATGGCAACATGGCAGGCATCTCAGACCAAAGGATCGCCATCGACCTGGTAACCACTCCCTTGTCCACCCCCGACCCGTCCCCAGGGTCTGCCTCAGCACAGCCCCACCTCCACTTGCCCTTCCTACCCACCCCCCAATCTCATGTCCCAGCTTGGGGTGCTGAGTCTGCTCTTCGGCCTGGGTGGGATACACAGAATGCCTAGTTTCATGGATGCCAGCTGGAGAGCACGGCACCTGGCAGACACTTACTGGGCAGGGGGGATCCCAAGAGCAGCCATGGGGTGAGCCCCACTCCCGCTGACACCAGAGACAGGGGAGACATGTGCTGCGGTCTGGGAAATAGCTAC = SEQUENCE_ID=HCF2_X5 SEQUENCE_TARGET=298,850 SEQUENCE_TEMPLATE=CCCGGAGAAGTGCGCAGCAGTGTGGGGAGCTGGAGCTGGGGTGGCTGTCCTGCACCAGCCCCCACGACCCTCAGACCACAGGCACTGCCAAGAGGGAACATGAACCTAGCCGGCCTCTAAGTGCAACGGCTGCCCCTGACAGGTGGTGACAGATATTTTCAAGAGTGACTCTGACCAGCTGTGATTTCCACCTTACATGTTGTCTTTGGATCCTTTCCCTGAATGATATGAGATTGTGCTGGGAACTCTAGCCCTCTGTGTGCTGACCTCCAGAATCTGACAACTTTCCTTTCCAAACAGTTCAAGCACCAAGGCACGATCACAGTGAACGAGGAAGGCACCCAAGCCACCACTGTGACCACGGTGGGGTTCATGCCGCTGTCCACCCAAGTCCGCTTCACTGTCGACCGCCCCTTTCTTTTCCTCATCTACGAGCATCGCACCAGCTGCCTGCTCTTCATGGGAAGAGTGGCCAACCCCAGCAGGTCCTAGAGGTGGAGGTCTAGGTGTCTGAAGTGCCTTGGGGGCACCCTCATTTTGTTTCCATTCCAACAACGAGAACAGAGATGTTCTGGCATCATTTACGTAGTTTACGCTACCAATCTGAATTCGAGGCCCATATGAGAGGAGCTTAGAAACGACCAAGAAGAGAGGCTTGTTGGAATCAATTCTGCACAATAGCCCATGCTGTAAGCTCATAGAAGTCACTGTAACTGTAGTGTGTCTGCTGTTACCTAGAGGGTCTCACCTCCCCACTCTTCACAGCAAACCTGAGCAGCGCGTCCTAAGCACCTCCCGCTCCGGTGACCCCATCCTTGCACACCTGACTCTGTCACTCAAGCCTTTCTCCACCAGGCCCCTCATCTGAATACCAAGCACAGAAATGAGTGGTGTGACTAATTCCTTACCTCTCCCAAGGAGGGTACACAACTAGCACCATTCTTGATGTCCAGGGAAGAAGCCACCTCAAGACATATGAGGGGTGCCCTGGGCTAATGTTAGGGCTTAATTTTCTCAAAGCCTGACCTTTCAAATCCATGATGAATGCCATCAGTCCCTCCTGCTGTTGCCTCCCTGTGACCTGGAGGACAGTGTGTGCCATGTCTCCCATACTAGAGATAAATAAATGTAGCCACATTTACTGTGTATCTGTTATAATTCTCTATTTTTTGAAGCTCAAATATCAAAAGCCAAATCCAAATTCCTGGATAACTCCAGGTATGATAAAGGCTGAGAGGAAGTCACTTGAGCACCACAATGTGCCACAGCAGGGCATGTTCTCAGGACAGGACAGGTGTGTGCTGAATCCTGGGGAGGGTCTGTGCAGTACCCCAGAACTGTGGGGTGCTAAGTGGCACACAAGCCCCAGGGCTCCCACAGTCTATGCCAGGCTGCTGCAGCTTTCATCCCTCATACCTGGTCCTGCAGTGGGTCTGGTTTGACAGA = SEQUENCE_ID=HMGCR_X1 SEQUENCE_TARGET=74,164 SEQUENCE_TEMPLATE=CCAGGCTGAGCGTCGGCGCCGGGGTTCGGTGGCCTCTAGTGAGATCTGGAGGATCCAAGGATTCTGTAGCTACAATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCCATCCCTGGGAAGTCATAGTGGGGACAGTGACACTGACCATCTGCATGATGTCCATGAACATGTTTACTGGTAACAATAAGATCTGTGGTTGGAATTATGAATGTCCAAAGTTTGAAGA = SEQUENCE_ID=HMGCR_X2 SEQUENCE_TARGET=0,112 SEQUENCE_TEMPLATE=GGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGCATAGCCATCCTGTATATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTG = SEQUENCE_ID=HMGCR_X3 SEQUENCE_TARGET=0,88 SEQUENCE_TEMPLATE=GGTATTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCACTTCTTAGACAAAGAATTGACAGGCTTGA = SEQUENCE_ID=HMGCR_X4 SEQUENCE_TARGET=0,85 SEQUENCE_TEMPLATE=ATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTAGCAAAGTTTGCCCTCAGTTCCAACTCACA = SEQUENCE_ID=HMGCR_X5 SEQUENCE_TARGET=0,106 SEQUENCE_TEMPLATE=GGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTTTAGGTCCTACGTTTACCCTCGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCA = SEQUENCE_ID=HMGCR_X6 SEQUENCE_TARGET=0,107 SEQUENCE_TEMPLATE=GGGGTACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTACTTCGTGTTCATGACTTTCTTCCCAGCTTGTGTGTCCTTGGTATTAGA = SEQUENCE_ID=HMGCR_X7 SEQUENCE_TARGET=0,117 SEQUENCE_TEMPLATE=GCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGCAGCTCAGCCATTTTGCCCGAGTTTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAAGATGATTAT = SEQUENCE_ID=HMGCR_X8 SEQUENCE_TARGET=0,161 SEQUENCE_TEMPLATE=GTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCAAAACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAGAATTGAACCAAGTGTTTCCCTCTGGCAGTTTTATCTCTCTA = SEQUENCE_ID=HMGCR_X9 SEQUENCE_TARGET=0,248 SEQUENCE_TEMPLATE=AAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAGTTTAGCTCTCCTTCTGGCTGTCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTAAAAAACCCTATCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTATGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAGAAAGAAAA = SEQUENCE_ID=HMGCR_X10 SEQUENCE_TARGET=0,90 SEQUENCE_TEMPLATE=GTTGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTGGTTGGTAACTCCTCCTTACTCGATACTTCA = SEQUENCE_ID=HMGCR_X11 SEQUENCE_TARGET=0,89 SEQUENCE_TEMPLATE=TCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGCCTAATGAAGAATGTCTACAGATACTTGGGAATGCAGA = SEQUENCE_ID=HMGCR_X12 SEQUENCE_TARGET=0,195 SEQUENCE_TEMPLATE=GAAAGGTGCAAAATTCCTTAGTGATGCTGAGATCATCCAGTTAGTCAATGCTAAGCATATCCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGTGGTGTATCTATTCGCCGACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTTACAGGGATTATAATTACTCCTT = SEQUENCE_ID=HMGCR_X13 SEQUENCE_TARGET=0,159 SEQUENCE_TEMPLATE=GGTGATGGGAGCTTGTTGTGAGAATGTTATTGGATATATGCCCATCCCTGTTGGAGTGGCAGGACCCCTTTGCTTAGATGAAAAAGAATTTCAGGTTCCAATGGCAACAACAGAAGGTTGTCTTGTGGCCAGCACCAATAGAGGCTGCAGAGCAATAGG = SEQUENCE_ID=HMGCR_X14 SEQUENCE_TARGET=0,158 SEQUENCE_TEMPLATE=TCTTGGTGGAGGTGCCAGCAGCCGAGTCCTTGCAGATGGGATGACTCGTGGCCCAGTTGTGCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGCCTGGCTCGAAACATCTGAAGGGTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCA = SEQUENCE_ID=HMGCR_X15 SEQUENCE_TARGET=0,106 SEQUENCE_TEMPLATE=GATTTGCACGTCTACAGAAACTTCATACAAGTATAGCTGGACGCAACCTTTATATCCGTTTCCAGTCCAGGTCAGGGGATGCCATGGGGATGAACATGATTTCAAA = SEQUENCE_ID=HMGCR_X16 SEQUENCE_TARGET=0,171 SEQUENCE_TEMPLATE=GGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGAAATGCAGATTCTAGCCGTTAGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAGGGAAGAGGAAAATCTGTTGTTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGA = SEQUENCE_ID=HMGCR_X17 SEQUENCE_TARGET=0,141 SEQUENCE_TEMPLATE=AGTATTAAAGACTACCACAGAGGCTATGATTGAGGTCAACATTAACAAGAATTTAGTGGGCTCTGCCATGGCTGGGAGCATAGGAGGCTACAACGCCCATGCAGCAAACATTGTCACCGCCATCTACATTGCCTGTGGACA = SEQUENCE_ID=HMGCR_X18 SEQUENCE_TARGET=0,159 SEQUENCE_TEMPLATE=GGATGCAGCACAGAATGTTGGTAGTTCAAACTGTATTACTTTAATGGAAGCAAGTGGTCCCACAAATGAAGATTTATATATCAGCTGCACCATGCCATCTATAGAGATAGGAACGGTGGGTGGTGGGACCAACCTACTACCTCAGCAAGCCTGTTTGCA = SEQUENCE_ID=HMGCR_X19 SEQUENCE_TARGET=0,155 SEQUENCE_TEMPLATE=GATGCTAGGTGTTCAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGCCCGAATTGTGTGTGGGACCGTAATGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGCAGGACATCTTGTCAAAAGTCACATGATTCACAACA = SEQUENCE_ID=HMGCR_X20 SEQUENCE_TARGET=0,56 SEQUENCE_TEMPLATE=GGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGAATAGCCCGACAGTTCTGAACTGGAACATGGGCATTGGGTTCTAAAGGACTAACATAAAATCTGTGAATTAAAAAAGCTCAATGCATTGTCTTGTGGAGGATGAATAAA = primer3-2.3.6/test/primer_new_tasks_output0000664000077300007730000025377012231156245017772 0ustar ioanaioanaSEQUENCE_ID=Check left only SEQUENCE_PRIMER=gtcctcgaacagctcagtcc PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.006937 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_LEFT_0=0,20 PRIMER_LEFT_0_TM=59.993 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.6000 = SEQUENCE_ID=Check internal only SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_INTERNAL_0_PENALTY=0.187324 PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa PRIMER_INTERNAL_0=90,20 PRIMER_INTERNAL_0_TM=60.187 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 = SEQUENCE_ID=Check right only SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_RIGHT_0=199,20 PRIMER_RIGHT_0_TM=60.006 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_RIGHT_0_END_STABILITY=7.0000 = SEQUENCE_ID=Pick the same primers as before SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.013026 PRIMER_LEFT_0_PENALTY=0.006937 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_INTERNAL_0_PENALTY=0.187324 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa PRIMER_LEFT_0=198,20 PRIMER_RIGHT_0=315,20 PRIMER_INTERNAL_0=250,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.006 PRIMER_INTERNAL_0_TM=60.187 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=118 = SEQUENCE_ID=Check primers with sequence SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.013026 PRIMER_LEFT_0_PENALTY=0.006937 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_INTERNAL_0_PENALTY=0.187324 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa PRIMER_LEFT_0=198,20 PRIMER_RIGHT_0=315,20 PRIMER_INTERNAL_0=250,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.006 PRIMER_INTERNAL_0_TM=60.187 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=118 = SEQUENCE_ID=Check primers without sequence PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.013026 PRIMER_LEFT_0_PENALTY=0.006937 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_INTERNAL_0_PENALTY=0.187324 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=199,20 PRIMER_INTERNAL_0=90,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.006 PRIMER_INTERNAL_0_TM=60.187 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=200 = SEQUENCE_ID=Check primers without sequence and internal PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.013026 PRIMER_LEFT_0_PENALTY=0.006937 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=199,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.006 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=200 = SEQUENCE_ID=Pick three times the same primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.000037 PRIMER_RIGHT_0_PENALTY=0.000037 PRIMER_INTERNAL_0_PENALTY=0.000037 PRIMER_LEFT_0_SEQUENCE=gacgtttgctttctgcttcc PRIMER_RIGHT_0_SEQUENCE=ggaagcagaaagcaaacgtc PRIMER_INTERNAL_0_SEQUENCE=gacgtttgctttctgcttcc PRIMER_LEFT_0=1471,20 PRIMER_RIGHT_0=1490,20 PRIMER_INTERNAL_0=1471,20 PRIMER_LEFT_0_TM=60.000 PRIMER_RIGHT_0_TM=60.000 PRIMER_INTERNAL_0_TM=60.000 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=7.8000 PRIMER_LEFT_1_PENALTY=0.000037 PRIMER_RIGHT_1_PENALTY=0.000037 PRIMER_INTERNAL_1_PENALTY=0.000037 PRIMER_LEFT_1_SEQUENCE=atcgccaccgtacaagaaac PRIMER_RIGHT_1_SEQUENCE=gtttcttgtacggtggcgat PRIMER_INTERNAL_1_SEQUENCE=atcgccaccgtacaagaaac PRIMER_LEFT_1=849,20 PRIMER_RIGHT_1=868,20 PRIMER_INTERNAL_1=849,20 PRIMER_LEFT_1_TM=60.000 PRIMER_RIGHT_1_TM=60.000 PRIMER_INTERNAL_1_TM=60.000 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=9.8000 PRIMER_LEFT_2_PENALTY=0.006089 PRIMER_RIGHT_2_PENALTY=0.006089 PRIMER_INTERNAL_2_PENALTY=0.006089 PRIMER_LEFT_2_SEQUENCE=aacaacggcaataaaaacgc PRIMER_RIGHT_2_SEQUENCE=gcgtttttattgccgttgtt PRIMER_INTERNAL_2_SEQUENCE=aacaacggcaataaaaacgc PRIMER_LEFT_2=296,20 PRIMER_RIGHT_2=315,20 PRIMER_INTERNAL_2=296,20 PRIMER_LEFT_2_TM=60.006 PRIMER_RIGHT_2_TM=60.006 PRIMER_INTERNAL_2_TM=60.006 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.9000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_LEFT_3_PENALTY=0.006937 PRIMER_RIGHT_3_PENALTY=0.006937 PRIMER_INTERNAL_3_PENALTY=0.006937 PRIMER_LEFT_3_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_3_SEQUENCE=ggactgagctgttcgaggac PRIMER_INTERNAL_3_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_LEFT_3=198,20 PRIMER_RIGHT_3=217,20 PRIMER_INTERNAL_3=198,20 PRIMER_LEFT_3_TM=59.993 PRIMER_RIGHT_3_TM=59.993 PRIMER_INTERNAL_3_TM=59.993 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_LEFT_4_PENALTY=0.014419 PRIMER_RIGHT_4_PENALTY=0.014419 PRIMER_INTERNAL_4_PENALTY=0.014419 PRIMER_LEFT_4_SEQUENCE=ttctctggacacagctccct PRIMER_RIGHT_4_SEQUENCE=agggagctgtgtccagagaa PRIMER_INTERNAL_4_SEQUENCE=ttctctggacacagctccct PRIMER_LEFT_4=39,20 PRIMER_RIGHT_4=58,20 PRIMER_INTERNAL_4=39,20 PRIMER_LEFT_4_TM=59.986 PRIMER_RIGHT_4_TM=59.986 PRIMER_INTERNAL_4_TM=59.986 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 = SEQUENCE_ID=Make a list of a small range SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatga PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=20 PRIMER_LEFT_NUM_RETURNED=6 PRIMER_RIGHT_NUM_RETURNED=17 PRIMER_INTERNAL_NUM_RETURNED=20 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=1.277576 PRIMER_RIGHT_0_PENALTY=0.557487 PRIMER_INTERNAL_0_PENALTY=0.300301 PRIMER_LEFT_0_SEQUENCE=gatttgatggacatggatgc PRIMER_RIGHT_0_SEQUENCE=ggcatccatgtccatcaaat PRIMER_INTERNAL_0_SEQUENCE=gacatggatgccatcaatga PRIMER_LEFT_0=3,20 PRIMER_RIGHT_0=23,20 PRIMER_INTERNAL_0=12,20 PRIMER_LEFT_0_TM=58.722 PRIMER_RIGHT_0_TM=60.557 PRIMER_INTERNAL_0_TM=60.300 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=11.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=9.00 PRIMER_LEFT_0_END_STABILITY=8.1000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_LEFT_1_PENALTY=1.436758 PRIMER_RIGHT_1_PENALTY=1.277576 PRIMER_INTERNAL_1_PENALTY=0.557487 PRIMER_LEFT_1_SEQUENCE=tggatttgatggacatggat PRIMER_RIGHT_1_SEQUENCE=gcatccatgtccatcaaatc PRIMER_INTERNAL_1_SEQUENCE=atttgatggacatggatgcc PRIMER_LEFT_1=1,20 PRIMER_RIGHT_1=22,20 PRIMER_INTERNAL_1=4,20 PRIMER_LEFT_1_TM=58.563 PRIMER_RIGHT_1_TM=58.722 PRIMER_INTERNAL_1_TM=60.557 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.1000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_LEFT_2_PENALTY=1.436758 PRIMER_RIGHT_2_PENALTY=1.283829 PRIMER_INTERNAL_2_PENALTY=1.277576 PRIMER_LEFT_2_SEQUENCE=atggatttgatggacatgga PRIMER_RIGHT_2_SEQUENCE=ggcatccatgtccatcaaa PRIMER_INTERNAL_2_SEQUENCE=gatttgatggacatggatgc PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=23,19 PRIMER_INTERNAL_2=3,20 PRIMER_LEFT_2_TM=58.563 PRIMER_RIGHT_2_TM=60.284 PRIMER_INTERNAL_2_TM=58.722 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=47.368 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=7.3000 PRIMER_LEFT_3_PENALTY=2.074289 PRIMER_RIGHT_3_PENALTY=1.436758 PRIMER_INTERNAL_3_PENALTY=1.283829 PRIMER_LEFT_3_SEQUENCE=atggatttgatggacatggat PRIMER_RIGHT_3_SEQUENCE=catccatgtccatcaaatcc PRIMER_INTERNAL_3_SEQUENCE=tttgatggacatggatgcc PRIMER_LEFT_3=0,21 PRIMER_RIGHT_3=21,20 PRIMER_INTERNAL_3=5,19 PRIMER_LEFT_3_TM=58.926 PRIMER_RIGHT_3_TM=58.563 PRIMER_INTERNAL_3_TM=60.284 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=47.368 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_LEFT_4_PENALTY=2.844552 PRIMER_RIGHT_4_PENALTY=1.436758 PRIMER_INTERNAL_4_PENALTY=1.331130 PRIMER_LEFT_4_SEQUENCE=tggatttgatggacatgga PRIMER_RIGHT_4_SEQUENCE=atccatgtccatcaaatcca PRIMER_INTERNAL_4_SEQUENCE=gatggacatggatgccatc PRIMER_LEFT_4=1,19 PRIMER_RIGHT_4=20,20 PRIMER_INTERNAL_4=8,19 PRIMER_LEFT_4_TM=58.155 PRIMER_RIGHT_4_TM=58.563 PRIMER_INTERNAL_4_TM=59.669 PRIMER_LEFT_4_GC_PERCENT=42.105 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=52.632 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=10.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=10.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_LEFT_5_PENALTY=3.413493 PRIMER_RIGHT_5_PENALTY=1.436758 PRIMER_INTERNAL_5_PENALTY=1.436758 PRIMER_LEFT_5_SEQUENCE=ggatttgatggacatggatgc PRIMER_RIGHT_5_SEQUENCE=tccatgtccatcaaatccat PRIMER_INTERNAL_5_SEQUENCE=ggatttgatggacatggatg PRIMER_LEFT_5=2,21 PRIMER_RIGHT_5=19,20 PRIMER_INTERNAL_5=2,20 PRIMER_LEFT_5_TM=62.413 PRIMER_RIGHT_5_TM=58.563 PRIMER_INTERNAL_5_TM=58.563 PRIMER_LEFT_5_GC_PERCENT=47.619 PRIMER_RIGHT_5_GC_PERCENT=40.000 PRIMER_INTERNAL_5_GC_PERCENT=45.000 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_INTERNAL_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=2.00 PRIMER_RIGHT_5_SELF_END=2.00 PRIMER_INTERNAL_5_SELF_END=4.00 PRIMER_LEFT_5_END_STABILITY=8.1000 PRIMER_RIGHT_5_END_STABILITY=8.1000 PRIMER_RIGHT_6_PENALTY=1.742755 PRIMER_INTERNAL_6_PENALTY=1.436758 PRIMER_RIGHT_6_SEQUENCE=atggcatccatgtccatca PRIMER_INTERNAL_6_SEQUENCE=tggatttgatggacatggat PRIMER_RIGHT_6=25,19 PRIMER_INTERNAL_6=1,20 PRIMER_RIGHT_6_TM=60.743 PRIMER_INTERNAL_6_TM=58.563 PRIMER_RIGHT_6_GC_PERCENT=47.368 PRIMER_INTERNAL_6_GC_PERCENT=40.000 PRIMER_RIGHT_6_SELF_ANY=8.00 PRIMER_INTERNAL_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_INTERNAL_6_SELF_END=2.00 PRIMER_RIGHT_6_END_STABILITY=6.9000 PRIMER_RIGHT_7_PENALTY=2.074289 PRIMER_INTERNAL_7_PENALTY=1.436758 PRIMER_RIGHT_7_SEQUENCE=atccatgtccatcaaatccat PRIMER_INTERNAL_7_SEQUENCE=atggatttgatggacatgga PRIMER_RIGHT_7=20,21 PRIMER_INTERNAL_7=0,20 PRIMER_RIGHT_7_TM=58.926 PRIMER_INTERNAL_7_TM=58.563 PRIMER_RIGHT_7_GC_PERCENT=38.095 PRIMER_INTERNAL_7_GC_PERCENT=40.000 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_INTERNAL_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_INTERNAL_7_SELF_END=2.00 PRIMER_RIGHT_7_END_STABILITY=8.1000 PRIMER_RIGHT_8_PENALTY=2.134627 PRIMER_INTERNAL_8_PENALTY=1.742755 PRIMER_RIGHT_8_SEQUENCE=atggcatccatgtccatcaa PRIMER_INTERNAL_8_SEQUENCE=atggacatggatgccatca PRIMER_RIGHT_8=25,20 PRIMER_INTERNAL_8=9,19 PRIMER_RIGHT_8_TM=62.135 PRIMER_INTERNAL_8_TM=60.743 PRIMER_RIGHT_8_GC_PERCENT=45.000 PRIMER_INTERNAL_8_GC_PERCENT=47.368 PRIMER_RIGHT_8_SELF_ANY=8.00 PRIMER_INTERNAL_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_INTERNAL_8_SELF_END=5.00 PRIMER_RIGHT_8_END_STABILITY=6.9000 PRIMER_RIGHT_9_PENALTY=2.454729 PRIMER_INTERNAL_9_PENALTY=1.742755 PRIMER_RIGHT_9_SEQUENCE=tggcatccatgtccatca PRIMER_INTERNAL_9_SEQUENCE=tgatggacatggatgccat PRIMER_RIGHT_9=24,18 PRIMER_INTERNAL_9=7,19 PRIMER_RIGHT_9_TM=60.455 PRIMER_INTERNAL_9_TM=60.743 PRIMER_RIGHT_9_GC_PERCENT=50.000 PRIMER_INTERNAL_9_GC_PERCENT=47.368 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_INTERNAL_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_END=1.00 PRIMER_INTERNAL_9_SELF_END=8.00 PRIMER_RIGHT_9_END_STABILITY=6.9000 PRIMER_RIGHT_10_PENALTY=2.527737 PRIMER_INTERNAL_10_PENALTY=1.891046 PRIMER_RIGHT_10_SEQUENCE=catccatgtccatcaaatcca PRIMER_INTERNAL_10_SEQUENCE=ggacatggatgccatcaat PRIMER_RIGHT_10=21,21 PRIMER_INTERNAL_10=11,19 PRIMER_RIGHT_10_TM=61.528 PRIMER_INTERNAL_10_TM=59.109 PRIMER_RIGHT_10_GC_PERCENT=42.857 PRIMER_INTERNAL_10_GC_PERCENT=47.368 PRIMER_RIGHT_10_SELF_ANY=4.00 PRIMER_INTERNAL_10_SELF_ANY=9.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_INTERNAL_10_SELF_END=9.00 PRIMER_RIGHT_10_END_STABILITY=8.1000 PRIMER_RIGHT_11_PENALTY=2.844552 PRIMER_INTERNAL_11_PENALTY=2.074289 PRIMER_RIGHT_11_SEQUENCE=tccatgtccatcaaatcca PRIMER_INTERNAL_11_SEQUENCE=atggatttgatggacatggat PRIMER_RIGHT_11=19,19 PRIMER_INTERNAL_11=0,21 PRIMER_RIGHT_11_TM=58.155 PRIMER_INTERNAL_11_TM=58.926 PRIMER_RIGHT_11_GC_PERCENT=42.105 PRIMER_INTERNAL_11_GC_PERCENT=38.095 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_INTERNAL_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_INTERNAL_11_SELF_END=2.00 PRIMER_RIGHT_11_END_STABILITY=8.1000 PRIMER_RIGHT_12_PENALTY=2.951135 PRIMER_INTERNAL_12_PENALTY=2.134627 PRIMER_RIGHT_12_SEQUENCE=tggcatccatgtccatcaa PRIMER_INTERNAL_12_SEQUENCE=ggacatggatgccatcaatg PRIMER_RIGHT_12=24,19 PRIMER_INTERNAL_12=11,20 PRIMER_RIGHT_12_TM=61.951 PRIMER_INTERNAL_12_TM=62.135 PRIMER_RIGHT_12_GC_PERCENT=47.368 PRIMER_INTERNAL_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_SELF_ANY=6.00 PRIMER_INTERNAL_12_SELF_ANY=11.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_INTERNAL_12_SELF_END=11.00 PRIMER_RIGHT_12_END_STABILITY=6.9000 PRIMER_RIGHT_13_PENALTY=3.275586 PRIMER_INTERNAL_13_PENALTY=2.134627 PRIMER_RIGHT_13_SEQUENCE=ggcatccatgtccatcaa PRIMER_INTERNAL_13_SEQUENCE=tggacatggatgccatcaat PRIMER_RIGHT_13=23,18 PRIMER_INTERNAL_13=10,20 PRIMER_RIGHT_13_TM=58.724 PRIMER_INTERNAL_13_TM=62.135 PRIMER_RIGHT_13_GC_PERCENT=50.000 PRIMER_INTERNAL_13_GC_PERCENT=45.000 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_INTERNAL_13_SELF_ANY=9.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_INTERNAL_13_SELF_END=9.00 PRIMER_RIGHT_13_END_STABILITY=6.9000 PRIMER_RIGHT_14_PENALTY=3.413493 PRIMER_INTERNAL_14_PENALTY=2.134627 PRIMER_RIGHT_14_SEQUENCE=ggcatccatgtccatcaaatc PRIMER_INTERNAL_14_SEQUENCE=atggacatggatgccatcaa PRIMER_RIGHT_14=23,21 PRIMER_INTERNAL_14=9,20 PRIMER_RIGHT_14_TM=62.413 PRIMER_INTERNAL_14_TM=62.135 PRIMER_RIGHT_14_GC_PERCENT=47.619 PRIMER_INTERNAL_14_GC_PERCENT=45.000 PRIMER_RIGHT_14_SELF_ANY=4.00 PRIMER_INTERNAL_14_SELF_ANY=8.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_INTERNAL_14_SELF_END=7.00 PRIMER_RIGHT_14_END_STABILITY=6.9000 PRIMER_RIGHT_15_PENALTY=3.413493 PRIMER_INTERNAL_15_PENALTY=2.134627 PRIMER_RIGHT_15_SEQUENCE=gcatccatgtccatcaaatcc PRIMER_INTERNAL_15_SEQUENCE=ttgatggacatggatgccat PRIMER_RIGHT_15=22,21 PRIMER_INTERNAL_15=6,20 PRIMER_RIGHT_15_TM=62.413 PRIMER_INTERNAL_15_TM=62.135 PRIMER_RIGHT_15_GC_PERCENT=47.619 PRIMER_INTERNAL_15_GC_PERCENT=45.000 PRIMER_RIGHT_15_SELF_ANY=4.00 PRIMER_INTERNAL_15_SELF_ANY=8.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_INTERNAL_15_SELF_END=8.00 PRIMER_RIGHT_15_END_STABILITY=8.1000 PRIMER_RIGHT_16_PENALTY=3.719699 PRIMER_INTERNAL_16_PENALTY=2.454729 PRIMER_RIGHT_16_SEQUENCE=catccatgtccatcaaatccat PRIMER_INTERNAL_16_SEQUENCE=tggacatggatgccatca PRIMER_RIGHT_16=21,22 PRIMER_INTERNAL_16=10,18 PRIMER_RIGHT_16_TM=61.720 PRIMER_INTERNAL_16_TM=60.455 PRIMER_RIGHT_16_GC_PERCENT=40.909 PRIMER_INTERNAL_16_GC_PERCENT=50.000 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_INTERNAL_16_SELF_ANY=7.00 PRIMER_RIGHT_16_SELF_END=2.00 PRIMER_INTERNAL_16_SELF_END=5.00 PRIMER_RIGHT_16_END_STABILITY=8.1000 PRIMER_INTERNAL_17_PENALTY=2.454729 PRIMER_INTERNAL_17_SEQUENCE=tgatggacatggatgcca PRIMER_INTERNAL_17=7,18 PRIMER_INTERNAL_17_TM=60.455 PRIMER_INTERNAL_17_GC_PERCENT=50.000 PRIMER_INTERNAL_17_SELF_ANY=6.00 PRIMER_INTERNAL_17_SELF_END=6.00 PRIMER_INTERNAL_18_PENALTY=2.527737 PRIMER_INTERNAL_18_SEQUENCE=tggatttgatggacatggatg PRIMER_INTERNAL_18=1,21 PRIMER_INTERNAL_18_TM=61.528 PRIMER_INTERNAL_18_GC_PERCENT=42.857 PRIMER_INTERNAL_18_SELF_ANY=4.00 PRIMER_INTERNAL_18_SELF_END=4.00 PRIMER_INTERNAL_19_PENALTY=2.756256 PRIMER_INTERNAL_19_SEQUENCE=gatggacatggatgccatca PRIMER_INTERNAL_19=8,20 PRIMER_INTERNAL_19_TM=62.756 PRIMER_INTERNAL_19_GC_PERCENT=50.000 PRIMER_INTERNAL_19_SELF_ANY=10.00 PRIMER_INTERNAL_19_SELF_END=6.00 = SEQUENCE_ID=Make a list of a small range SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatga PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=2 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.557487 PRIMER_RIGHT_0_SEQUENCE=ggcatccatgtccatcaaat PRIMER_RIGHT_0=23,20 PRIMER_RIGHT_0_TM=60.557 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_RIGHT_1_PENALTY=1.277576 PRIMER_RIGHT_1_SEQUENCE=gcatccatgtccatcaaatc PRIMER_RIGHT_1=22,20 PRIMER_RIGHT_1_TM=58.722 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_RIGHT_1_END_STABILITY=6.9000 = SEQUENCE_ID=Check left only too small SEQUENCE_PRIMER=gtcctcgaaca PRIMER_TASK=check_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=Specified left primer < PRIMER_MIN_SIZE PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=41.742994 PRIMER_LEFT_0_PROBLEMS= Temperature too low; Too short; PRIMER_LEFT_0_SEQUENCE=gtcctcgaaca PRIMER_LEFT_0=0,11 PRIMER_LEFT_0_TM=27.257 PRIMER_LEFT_0_GC_PERCENT=54.545 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.7000 = SEQUENCE_ID=Check left only too big SEQUENCE_PRIMER=tgacctccacttctctggacacagctccctcaccaa PRIMER_TASK=check_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=Specified left primer > PRIMER_MAX_SIZE PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=36.812317 PRIMER_LEFT_0_PROBLEMS= Temperature too high; Too long; PRIMER_LEFT_0_SEQUENCE=tgacctccacttctctggacacagctccctcaccaa PRIMER_LEFT_0=0,36 PRIMER_LEFT_0_TM=80.812 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 = SEQUENCE_ID=Pick sequencing primers no target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=50 PRIMER_LEFT_NUM_RETURNED=4 PRIMER_RIGHT_NUM_RETURNED=4 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=1.134011 PRIMER_RIGHT_0_PENALTY=0.006937 PRIMER_LEFT_0_SEQUENCE=atgccatcaatgacctcca PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_0=19,19 PRIMER_RIGHT_0=217,20 PRIMER_LEFT_0_TM=59.866 PRIMER_RIGHT_0_TM=59.993 PRIMER_LEFT_0_GC_PERCENT=47.368 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_LEFT_1_PENALTY=0.120245 PRIMER_RIGHT_1_PENALTY=0.185267 PRIMER_LEFT_1_SEQUENCE=acgcatgaagttggtgatga PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_1=312,20 PRIMER_RIGHT_1=697,20 PRIMER_LEFT_1_TM=60.120 PRIMER_RIGHT_1_TM=60.185 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_LEFT_2_PENALTY=0.077340 PRIMER_RIGHT_2_PENALTY=0.051345 PRIMER_LEFT_2_SEQUENCE=catcttcggataaccgcatt PRIMER_RIGHT_2_SEQUENCE=gaaaaccagcctccaatgaa PRIMER_LEFT_2=801,20 PRIMER_RIGHT_2=1203,20 PRIMER_LEFT_2_TM=59.923 PRIMER_RIGHT_2_TM=60.051 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_LEFT_3_PENALTY=0.230279 PRIMER_RIGHT_3_PENALTY=2.744773 PRIMER_LEFT_3_SEQUENCE=ggggagtggttaggtgaggt PRIMER_RIGHT_3_SEQUENCE=gaagaagaacaagtccaaaggg PRIMER_LEFT_3=1317,20 PRIMER_RIGHT_3=1510,22 PRIMER_LEFT_3_TM=60.230 PRIMER_RIGHT_3_TM=59.255 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=45.455 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=9.7000 = SEQUENCE_ID=Pick sequencing primers small target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=450,37 PRIMER_NUM_RETURN=50 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.640220 PRIMER_RIGHT_0_PENALTY=0.501869 PRIMER_LEFT_0_SEQUENCE=aaccacaacaacaacaacgg PRIMER_RIGHT_0_SEQUENCE=aagtggccgaatttgacgta PRIMER_LEFT_0=284,20 PRIMER_RIGHT_0=670,20 PRIMER_LEFT_0_TM=59.360 PRIMER_RIGHT_0_TM=60.502 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.9000 PRIMER_RIGHT_0_END_STABILITY=7.1000 = SEQUENCE_ID=Pick sequencing primers left only SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=150,800 PRIMER_NUM_RETURN=50 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=1.134011 PRIMER_LEFT_0_SEQUENCE=atgccatcaatgacctcca PRIMER_LEFT_0=19,19 PRIMER_LEFT_0_TM=59.866 PRIMER_LEFT_0_GC_PERCENT=47.368 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_LEFT_1_PENALTY=0.405717 PRIMER_LEFT_1_SEQUENCE=agacttgccgctcatttcac PRIMER_LEFT_1=477,20 PRIMER_LEFT_1_TM=60.406 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 = SEQUENCE_ID=Pick sequencing primers right only SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 SEQUENCE_TARGET=150,800 PRIMER_NUM_RETURN=50 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.032351 PRIMER_RIGHT_0_SEQUENCE=tggaaagcagcaagtgtgtc PRIMER_RIGHT_0=628,20 PRIMER_RIGHT_0_TM=60.032 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_RIGHT_1_PENALTY=0.660780 PRIMER_RIGHT_1_SEQUENCE=cttcaaccaacgtcacaagc PRIMER_RIGHT_1=1107,20 PRIMER_RIGHT_1_TM=59.339 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_RIGHT_1_END_STABILITY=8.5000 = SEQUENCE_ID=Pick sequencing primers three target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=350,37 450,100 850,500 1200,100 PRIMER_NUM_RETURN=50 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.369208 PRIMER_RIGHT_0_PENALTY=0.298942 PRIMER_LEFT_0_SEQUENCE=ccatcatcgaggacagaaca PRIMER_RIGHT_0_SEQUENCE=ttgttgtggtgatggtggtt PRIMER_LEFT_0=175,20 PRIMER_RIGHT_0=562,20 PRIMER_LEFT_0_TM=59.631 PRIMER_RIGHT_0_TM=59.701 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_LEFT_1_PENALTY=0.006089 PRIMER_RIGHT_1_PENALTY=0.185267 PRIMER_LEFT_1_SEQUENCE=aacaacggcaataaaaacgc PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_1=296,20 PRIMER_RIGHT_1=697,20 PRIMER_LEFT_1_TM=60.006 PRIMER_RIGHT_1_TM=60.185 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.9000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_LEFT_2_PENALTY=0.262854 PRIMER_RIGHT_2_PENALTY=0.052187 PRIMER_LEFT_2_SEQUENCE=acttcacagccaatcaagcc PRIMER_RIGHT_2_SEQUENCE=cggtgcacggtaactaaggt PRIMER_LEFT_2=667,20 PRIMER_RIGHT_2=1040,20 PRIMER_LEFT_2_TM=60.263 PRIMER_RIGHT_2_TM=60.052 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_LEFT_3_PENALTY=0.061961 PRIMER_RIGHT_3_PENALTY=2.744773 PRIMER_LEFT_3_SEQUENCE=agcggtttttgattcattgg PRIMER_RIGHT_3_SEQUENCE=gaagaagaacaagtccaaaggg PRIMER_LEFT_3=1172,20 PRIMER_RIGHT_3=1510,22 PRIMER_LEFT_3_TM=59.938 PRIMER_RIGHT_3_TM=59.255 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=45.455 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.4000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_LEFT_4_PENALTY=0.248900 PRIMER_RIGHT_4_PENALTY=0.040772 PRIMER_LEFT_4_SEQUENCE=cggaatcagttgcttcgttt PRIMER_RIGHT_4_SEQUENCE=cacccacctcttcaacccta PRIMER_LEFT_4=1039,20 PRIMER_RIGHT_4=1434,20 PRIMER_LEFT_4_TM=60.249 PRIMER_RIGHT_4_TM=59.959 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.7000 PRIMER_RIGHT_4_END_STABILITY=8.7000 = SEQUENCE_ID=Pick fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 SEQUENCE_FORCE_LEFT_END=34 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=0.172685 PRIMER_LEFT_0_PENALTY=0.165748 PRIMER_RIGHT_0_PENALTY=0.006937 PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high; PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_0=15,20 PRIMER_RIGHT_0=217,20 PRIMER_LEFT_0_TM=60.166 PRIMER_RIGHT_0_TM=59.993 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=203 PRIMER_PAIR_1_PENALTY=0.196791 PRIMER_LEFT_1_PENALTY=0.165748 PRIMER_RIGHT_1_PENALTY=0.031043 PRIMER_LEFT_1_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high; PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_1_SEQUENCE=gttgacgatgggagaccagt PRIMER_LEFT_1=15,20 PRIMER_RIGHT_1=125,20 PRIMER_LEFT_1_TM=60.166 PRIMER_RIGHT_1_TM=59.969 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=9.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=111 PRIMER_PAIR_2_PENALTY=0.277562 PRIMER_LEFT_2_PENALTY=0.165748 PRIMER_RIGHT_2_PENALTY=0.111814 PRIMER_LEFT_2_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high; PRIMER_LEFT_2_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_2_SEQUENCE=agttgacgatgggagaccag PRIMER_LEFT_2=15,20 PRIMER_RIGHT_2=126,20 PRIMER_LEFT_2_TM=60.166 PRIMER_RIGHT_2_TM=60.112 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=9.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.6000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=112 = SEQUENCE_ID=Pick fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_START=697 SEQUENCE_FORCE_RIGHT_END=678 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=0.233855 PRIMER_LEFT_0_PENALTY=0.048588 PRIMER_RIGHT_0_PENALTY=0.185267 PRIMER_LEFT_0_SEQUENCE=cgacaaccaaaacgacacac PRIMER_RIGHT_0_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_0=596,20 PRIMER_RIGHT_0=697,20 PRIMER_LEFT_0_TM=60.049 PRIMER_RIGHT_0_TM=60.185 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=102 PRIMER_PAIR_1_PENALTY=0.233855 PRIMER_LEFT_1_PENALTY=0.048588 PRIMER_RIGHT_1_PENALTY=0.185267 PRIMER_LEFT_1_SEQUENCE=acgacaaccaaaacgacaca PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_1=595,20 PRIMER_RIGHT_1=697,20 PRIMER_LEFT_1_TM=60.049 PRIMER_RIGHT_1_TM=60.185 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=103 PRIMER_PAIR_2_PENALTY=0.233855 PRIMER_LEFT_2_PENALTY=0.048588 PRIMER_RIGHT_2_PENALTY=0.185267 PRIMER_LEFT_2_SEQUENCE=cacgacaaccaaaacgacac PRIMER_RIGHT_2_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_2=594,20 PRIMER_RIGHT_2=697,20 PRIMER_LEFT_2_TM=60.049 PRIMER_RIGHT_2_TM=60.185 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.1000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=104 = SEQUENCE_ID=Pick start fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=0.172685 PRIMER_LEFT_0_PENALTY=0.165748 PRIMER_RIGHT_0_PENALTY=0.006937 PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high; PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_0=15,20 PRIMER_RIGHT_0=217,20 PRIMER_LEFT_0_TM=60.166 PRIMER_RIGHT_0_TM=59.993 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=203 PRIMER_PAIR_1_PENALTY=0.196791 PRIMER_LEFT_1_PENALTY=0.165748 PRIMER_RIGHT_1_PENALTY=0.031043 PRIMER_LEFT_1_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high; PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_1_SEQUENCE=gttgacgatgggagaccagt PRIMER_LEFT_1=15,20 PRIMER_RIGHT_1=125,20 PRIMER_LEFT_1_TM=60.166 PRIMER_RIGHT_1_TM=59.969 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=9.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=111 PRIMER_PAIR_2_PENALTY=0.277562 PRIMER_LEFT_2_PENALTY=0.165748 PRIMER_RIGHT_2_PENALTY=0.111814 PRIMER_LEFT_2_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high; PRIMER_LEFT_2_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_2_SEQUENCE=agttgacgatgggagaccag PRIMER_LEFT_2=15,20 PRIMER_RIGHT_2=126,20 PRIMER_LEFT_2_TM=60.166 PRIMER_RIGHT_2_TM=60.112 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=9.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.6000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=112 = SEQUENCE_ID=Pick start fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_START=697 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=0.233855 PRIMER_LEFT_0_PENALTY=0.048588 PRIMER_RIGHT_0_PENALTY=0.185267 PRIMER_LEFT_0_SEQUENCE=cgacaaccaaaacgacacac PRIMER_RIGHT_0_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_0=596,20 PRIMER_RIGHT_0=697,20 PRIMER_LEFT_0_TM=60.049 PRIMER_RIGHT_0_TM=60.185 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=102 PRIMER_PAIR_1_PENALTY=0.233855 PRIMER_LEFT_1_PENALTY=0.048588 PRIMER_RIGHT_1_PENALTY=0.185267 PRIMER_LEFT_1_SEQUENCE=acgacaaccaaaacgacaca PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_1=595,20 PRIMER_RIGHT_1=697,20 PRIMER_LEFT_1_TM=60.049 PRIMER_RIGHT_1_TM=60.185 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=103 PRIMER_PAIR_2_PENALTY=0.233855 PRIMER_LEFT_2_PENALTY=0.048588 PRIMER_RIGHT_2_PENALTY=0.185267 PRIMER_LEFT_2_SEQUENCE=cacgacaaccaaaacgacac PRIMER_RIGHT_2_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_2=594,20 PRIMER_RIGHT_2=697,20 PRIMER_LEFT_2_TM=60.049 PRIMER_RIGHT_2_TM=60.185 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.1000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=104 = SEQUENCE_ID=Pick end fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_END=34 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=0.172685 PRIMER_LEFT_0_PENALTY=0.165748 PRIMER_RIGHT_0_PENALTY=0.006937 PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high; PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_0=15,20 PRIMER_RIGHT_0=217,20 PRIMER_LEFT_0_TM=60.166 PRIMER_RIGHT_0_TM=59.993 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=203 PRIMER_PAIR_1_PENALTY=0.196791 PRIMER_LEFT_1_PENALTY=0.165748 PRIMER_RIGHT_1_PENALTY=0.031043 PRIMER_LEFT_1_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high; PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_1_SEQUENCE=gttgacgatgggagaccagt PRIMER_LEFT_1=15,20 PRIMER_RIGHT_1=125,20 PRIMER_LEFT_1_TM=60.166 PRIMER_RIGHT_1_TM=59.969 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=9.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=111 = SEQUENCE_ID=Pick end fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_END=678 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=0.233855 PRIMER_LEFT_0_PENALTY=0.048588 PRIMER_RIGHT_0_PENALTY=0.185267 PRIMER_LEFT_0_SEQUENCE=cgacaaccaaaacgacacac PRIMER_RIGHT_0_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_0=596,20 PRIMER_RIGHT_0=697,20 PRIMER_LEFT_0_TM=60.049 PRIMER_RIGHT_0_TM=60.185 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=102 PRIMER_PAIR_1_PENALTY=0.233855 PRIMER_LEFT_1_PENALTY=0.048588 PRIMER_RIGHT_1_PENALTY=0.185267 PRIMER_LEFT_1_SEQUENCE=acgacaaccaaaacgacaca PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_1=595,20 PRIMER_RIGHT_1=697,20 PRIMER_LEFT_1_TM=60.049 PRIMER_RIGHT_1_TM=60.185 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=103 = SEQUENCE_ID=Pick cloning primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_cloning_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_INCLUDED_REGION=15,203 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=0.172685 PRIMER_LEFT_0_PENALTY=0.165748 PRIMER_RIGHT_0_PENALTY=0.006937 PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high; PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_0=15,20 PRIMER_RIGHT_0=217,20 PRIMER_LEFT_0_TM=60.166 PRIMER_RIGHT_0_TM=59.993 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=203 PRIMER_PAIR_1_PENALTY=1.897984 PRIMER_LEFT_1_PENALTY=1.891046 PRIMER_RIGHT_1_PENALTY=0.006937 PRIMER_LEFT_1_PROBLEMS= Similarity to self too high; PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacc PRIMER_RIGHT_1_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_1=15,19 PRIMER_RIGHT_1=217,20 PRIMER_LEFT_1_TM=59.109 PRIMER_RIGHT_1_TM=59.993 PRIMER_LEFT_1_GC_PERCENT=47.368 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=9.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=203 = SEQUENCE_ID=Pick cloning primers with only right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_cloning_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 SEQUENCE_INCLUDED_REGION=15,203 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.006937 PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac PRIMER_RIGHT_0=217,20 PRIMER_RIGHT_0_TM=59.993 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_RIGHT_1_PENALTY=1.938271 PRIMER_RIGHT_1_SEQUENCE=ggactgagctgttcgagga PRIMER_RIGHT_1=217,19 PRIMER_RIGHT_1_TM=59.062 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_RIGHT_1_END_STABILITY=7.9000 = SEQUENCE_ID=Pick discriminative primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_discriminative_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=35,163 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=2.740403 PRIMER_LEFT_0_PENALTY=1.868962 PRIMER_RIGHT_0_PENALTY=0.871441 PRIMER_LEFT_0_PROBLEMS= Overlaps target; Similary to 3' end of self too high; PRIMER_RIGHT_0_PROBLEMS= Overlaps target; Similary to 3' end of self too high; PRIMER_LEFT_0_SEQUENCE=tggatgccatcaatgacctc PRIMER_RIGHT_0_SEQUENCE=gactgagctgttcgaggaca PRIMER_LEFT_0=16,20 PRIMER_RIGHT_0=216,20 PRIMER_LEFT_0_TM=61.869 PRIMER_RIGHT_0_TM=59.129 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=201 PRIMER_PAIR_1_PENALTY=3.056129 PRIMER_LEFT_1_PENALTY=2.184689 PRIMER_RIGHT_1_PENALTY=0.871441 PRIMER_LEFT_1_PROBLEMS= Overlaps target; Similary to 3' end of self too high; PRIMER_RIGHT_1_PROBLEMS= Overlaps target; Similary to 3' end of self too high; PRIMER_LEFT_1_SEQUENCE=ggatgccatcaatgacctc PRIMER_RIGHT_1_SEQUENCE=gactgagctgttcgaggaca PRIMER_LEFT_1=17,19 PRIMER_RIGHT_1=216,20 PRIMER_LEFT_1_TM=58.815 PRIMER_RIGHT_1_TM=59.129 PRIMER_LEFT_1_GC_PERCENT=52.632 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=200 = SEQUENCE_ID=Pick start fixed left primer unique SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 PRIMER_NUM_RETURN=3 PRIMER_MIN_THREE_PRIME_DISTANCE=0 PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=0.172685 PRIMER_LEFT_0_PENALTY=0.165748 PRIMER_RIGHT_0_PENALTY=0.006937 PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high; PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_0=15,20 PRIMER_RIGHT_0=217,20 PRIMER_LEFT_0_TM=60.166 PRIMER_RIGHT_0_TM=59.993 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=203 PRIMER_PAIR_1_PENALTY=1.922089 PRIMER_LEFT_1_PENALTY=1.891046 PRIMER_RIGHT_1_PENALTY=0.031043 PRIMER_LEFT_1_PROBLEMS= Similarity to self too high; PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacc PRIMER_RIGHT_1_SEQUENCE=gttgacgatgggagaccagt PRIMER_LEFT_1=15,19 PRIMER_RIGHT_1=125,20 PRIMER_LEFT_1_TM=59.109 PRIMER_RIGHT_1_TM=59.969 PRIMER_LEFT_1_GC_PERCENT=47.368 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=9.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=111 PRIMER_PAIR_2_PENALTY=3.159700 PRIMER_LEFT_2_PENALTY=3.047886 PRIMER_RIGHT_2_PENALTY=0.111814 PRIMER_LEFT_2_PROBLEMS= Similarity to self too high; Similary to 3' end of self too high; PRIMER_LEFT_2_SEQUENCE=atggatgccatcaatgacctc PRIMER_RIGHT_2_SEQUENCE=agttgacgatgggagaccag PRIMER_LEFT_2=15,21 PRIMER_RIGHT_2=126,20 PRIMER_LEFT_2_TM=62.048 PRIMER_RIGHT_2_TM=60.112 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=9.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.6000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=112 = SEQUENCE_ID=Pick a left primer and an internal oligo SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.000037 PRIMER_INTERNAL_0_PENALTY=0.000037 PRIMER_LEFT_0_SEQUENCE=gacgtttgctttctgcttcc PRIMER_INTERNAL_0_SEQUENCE=gacgtttgctttctgcttcc PRIMER_LEFT_0=1471,20 PRIMER_INTERNAL_0=1471,20 PRIMER_LEFT_0_TM=60.000 PRIMER_INTERNAL_0_TM=60.000 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 = SEQUENCE_ID=Pick a right primer and an internal oligo SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=1 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.000037 PRIMER_INTERNAL_0_PENALTY=0.000037 PRIMER_RIGHT_0_SEQUENCE=ggaagcagaaagcaaacgtc PRIMER_INTERNAL_0_SEQUENCE=gacgtttgctttctgcttcc PRIMER_RIGHT_0=1490,20 PRIMER_INTERNAL_0=1471,20 PRIMER_RIGHT_0_TM=60.000 PRIMER_INTERNAL_0_TM=60.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_RIGHT_0_END_STABILITY=7.8000 = SEQUENCE_ID=Test behavior of SEQUENCE_PRIMER_PAIR_OK_REGION_LIST with PRIMER_TASK=pick_primer_list SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg PRIMER_TASK=pick_primer_list PRIMER_NUM_RETURN=15 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=40-100 PRIMER_MIN_GC=0 PRIMER_MIN_TM=0 PRIMER_PICK_ANYWAY=0 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_SIZE=16 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,20,49,17 PRIMER_LEFT_EXPLAIN=considered 954, not in any ok left region 939, ok 15 PRIMER_RIGHT_EXPLAIN=considered 954, not in any ok right region 951, ok 3 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=15 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=8.942519 PRIMER_RIGHT_0_PENALTY=17.049867 PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAg PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=65,17 PRIMER_LEFT_0_TM=51.057 PRIMER_RIGHT_0_TM=45.950 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=29.412 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_LEFT_1_PENALTY=11.546670 PRIMER_RIGHT_1_PENALTY=20.075362 PRIMER_LEFT_1_SEQUENCE=ttgattcaatgaatctcaa PRIMER_RIGHT_1_SEQUENCE=aaaaaaacacaatccA PRIMER_LEFT_1=2,19 PRIMER_RIGHT_1=65,16 PRIMER_LEFT_1_TM=49.453 PRIMER_RIGHT_1_TM=43.925 PRIMER_LEFT_1_GC_PERCENT=26.316 PRIMER_RIGHT_1_GC_PERCENT=25.000 PRIMER_LEFT_1_SELF_ANY=11.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_LEFT_2_PENALTY=12.075654 PRIMER_RIGHT_2_PENALTY=20.694887 PRIMER_LEFT_2_SEQUENCE=cttgattcaatgaatctca PRIMER_RIGHT_2_SEQUENCE=aaaaaacacaatccAg PRIMER_LEFT_2=1,19 PRIMER_RIGHT_2=64,16 PRIMER_LEFT_2_TM=48.924 PRIMER_RIGHT_2_TM=43.305 PRIMER_LEFT_2_GC_PERCENT=31.579 PRIMER_RIGHT_2_GC_PERCENT=31.250 PRIMER_LEFT_2_SELF_ANY=11.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=7.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_LEFT_3_PENALTY=14.913528 PRIMER_LEFT_3_SEQUENCE=tgattcaatgaatctcaa PRIMER_LEFT_3=3,18 PRIMER_LEFT_3_TM=47.086 PRIMER_LEFT_3_GC_PERCENT=27.778 PRIMER_LEFT_3_SELF_ANY=11.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_LEFT_4_PENALTY=14.913528 PRIMER_LEFT_4_SEQUENCE=ttgattcaatgaatctca PRIMER_LEFT_4=2,18 PRIMER_LEFT_4_TM=47.086 PRIMER_LEFT_4_GC_PERCENT=27.778 PRIMER_LEFT_4_SELF_ANY=11.00 PRIMER_LEFT_4_SELF_END=7.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_LEFT_5_PENALTY=16.846122 PRIMER_LEFT_5_SEQUENCE=cttgattcaatgaatctc PRIMER_LEFT_5=1,18 PRIMER_LEFT_5_TM=45.154 PRIMER_LEFT_5_GC_PERCENT=33.333 PRIMER_LEFT_5_SELF_ANY=11.00 PRIMER_LEFT_5_SELF_END=7.00 PRIMER_LEFT_5_END_STABILITY=6.3000 PRIMER_LEFT_6_PENALTY=18.624075 PRIMER_LEFT_6_SEQUENCE=tgattcaatgaatctca PRIMER_LEFT_6=3,17 PRIMER_LEFT_6_TM=44.376 PRIMER_LEFT_6_GC_PERCENT=29.412 PRIMER_LEFT_6_SELF_ANY=11.00 PRIMER_LEFT_6_SELF_END=5.00 PRIMER_LEFT_6_END_STABILITY=6.7000 PRIMER_LEFT_7_PENALTY=19.982933 PRIMER_LEFT_7_SEQUENCE=gattcaatgaatctcaa PRIMER_LEFT_7=4,17 PRIMER_LEFT_7_TM=43.017 PRIMER_LEFT_7_GC_PERCENT=29.412 PRIMER_LEFT_7_SELF_ANY=11.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_LEFT_7_END_STABILITY=7.0000 PRIMER_LEFT_8_PENALTY=19.982933 PRIMER_LEFT_8_SEQUENCE=ttgattcaatgaatctc PRIMER_LEFT_8=2,17 PRIMER_LEFT_8_TM=43.017 PRIMER_LEFT_8_GC_PERCENT=29.412 PRIMER_LEFT_8_SELF_ANY=11.00 PRIMER_LEFT_8_SELF_END=7.00 PRIMER_LEFT_8_END_STABILITY=6.3000 PRIMER_LEFT_9_PENALTY=20.744280 PRIMER_LEFT_9_SEQUENCE=cttgattcaatgaatct PRIMER_LEFT_9=1,17 PRIMER_LEFT_9_TM=42.256 PRIMER_LEFT_9_GC_PERCENT=29.412 PRIMER_LEFT_9_SELF_ANY=11.00 PRIMER_LEFT_9_SELF_END=9.00 PRIMER_LEFT_9_END_STABILITY=6.6000 PRIMER_LEFT_10_PENALTY=24.111010 PRIMER_LEFT_10_SEQUENCE=gattcaatgaatctca PRIMER_LEFT_10=4,16 PRIMER_LEFT_10_TM=39.889 PRIMER_LEFT_10_GC_PERCENT=31.250 PRIMER_LEFT_10_SELF_ANY=11.00 PRIMER_LEFT_10_SELF_END=4.00 PRIMER_LEFT_10_END_STABILITY=6.7000 PRIMER_LEFT_11_PENALTY=24.111010 PRIMER_LEFT_11_SEQUENCE=tgattcaatgaatctc PRIMER_LEFT_11=3,16 PRIMER_LEFT_11_TM=39.889 PRIMER_LEFT_11_GC_PERCENT=31.250 PRIMER_LEFT_11_SELF_ANY=11.00 PRIMER_LEFT_11_SELF_END=7.00 PRIMER_LEFT_11_END_STABILITY=6.3000 PRIMER_LEFT_12_PENALTY=24.152694 PRIMER_LEFT_12_SEQUENCE=attcaatgaatctcaa PRIMER_LEFT_12=5,16 PRIMER_LEFT_12_TM=39.847 PRIMER_LEFT_12_GC_PERCENT=25.000 PRIMER_LEFT_12_SELF_ANY=9.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_LEFT_12_END_STABILITY=7.0000 PRIMER_LEFT_13_PENALTY=24.152694 PRIMER_LEFT_13_SEQUENCE=ttgattcaatgaatct PRIMER_LEFT_13=2,16 PRIMER_LEFT_13_TM=39.847 PRIMER_LEFT_13_GC_PERCENT=25.000 PRIMER_LEFT_13_SELF_ANY=11.00 PRIMER_LEFT_13_SELF_END=9.00 PRIMER_LEFT_13_END_STABILITY=6.6000 PRIMER_LEFT_14_PENALTY=24.152694 PRIMER_LEFT_14_SEQUENCE=cttgattcaatgaatc PRIMER_LEFT_14=1,16 PRIMER_LEFT_14_TM=39.847 PRIMER_LEFT_14_GC_PERCENT=31.250 PRIMER_LEFT_14_SELF_ANY=11.00 PRIMER_LEFT_14_SELF_END=11.00 PRIMER_LEFT_14_END_STABILITY=6.6000 = primer3-2.3.6/test/primer1_input0000664000077300007730000001255412231156245015565 0ustar ioanaioanaPRIMER_FIRST_BASE_INDEX=1 PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 P3_FILE_FLAG=1 SEQUENCE_ID=targets_test SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=22,122 = SEQUENCE_ID=SP01-02.txt SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG = SEQUENCE_ID=SP01-03.txt SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC = SEQUENCE_ID=SP01-06.txt SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC = SEQUENCE_ID=SP01-15.txt SEQUENCE_TEMPLATE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC = SEQUENCE_ID=SP01-19.txt SEQUENCE_TEMPLATE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC = SEQUENCE_ID=SP01-20.txt SEQUENCE_TEMPLATE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC = SEQUENCE_ID=SP01-21.txt SEQUENCE_TEMPLATE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC = SEQUENCE_ID=SP03-40.txt SEQUENCE_TEMPLATE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC = SEQUENCE_ID=SP04-07.txt SEQUENCE_TEMPLATE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA = SEQUENCE_ID=AGAG SEQUENCE_TEMPLATE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA = SEQUENCE_ID=long_seq SEQUENCE_TEMPLATE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg = SEQUENCE_ID=bad_letter SEQUENCE_TEMPLATE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC = SEQUENCE_ID=bad_target SEQUENCE_TEMPLATE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC SEQUENCE_TARGET=51,10 SEQUENCE_TARGET=501,50 = SEQUENCE_ID=other_bad_target SEQUENCE_TEMPLATE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC SEQUENCE_TARGET=101,1000 = SEQUENCE_ID=included_test SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=21,228 = PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MAX_SELF_END=8 SEQUENCE_ID=included_test_w_middle SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=21,228 = primer3-2.3.6/test/thal_output0000775000077300007730000012266312231156245015345 0ustar ioanaioanaCalculated thermodynamical parameters for dimer: 16 dS = -146.99 dH = -50600 dG = -5011.14 t = 71.0918 SEQ //////----\\\\\\ STR CCGCAGTAAGCTGCGG Calculated thermodynamical parameters for dimer: 16 dS = -146.99 dH = -50600 dG = -5011.14 t = 71.0918 SEQ //////----\\\\\\ STR CCGCAGTAAGCTGCGG Calculated thermodynamical parameters for dimer: dS = -153.512 dH = -54000 dG = -6388.21 t = 11.5486 SEQ TAAGCTGCGG SEQ CCGCAG STR GGCGTC STR GAATGACGCC Calculated thermodynamical parameters for dimer: dS = -153.512 dH = -54000 dG = -6388.21 t = 11.5486 SEQ TAAGCTGCGG SEQ CCGCAG STR GGCGTC STR GAATGACGCC Calculated thermodynamical parameters for dimer: dS = -153.512 dH = -54000 dG = -6388.21 t = 11.5486 SEQ TAAGCTGCGG SEQ CCGCAG STR GGCGTC STR GAATGACGCC Calculated thermodynamical parameters for dimer: dS = -295.897 dH = -101000 dG = -20471.7 t = 31.0129 SEQ TAAG SEQ CCGCAG CTGCGG STR GGCGTC GACGCC STR GAAT Calculated thermodynamical parameters for dimer: dS = -280.782 dH = -93200 dG = -6115.6 t = 20.9085 SEQ AAAACCCGC TA- ---- SEQ TTTGCTAGC CG STR AAACGATCG GC STR AAAAA TTC CCAA Calculated thermodynamical parameters for dimer: dS = -229.719 dH = -79000 dG = -7752.52 t = 23.9748 SEQ AAAACCCGC TACG----- SEQ TTTGCTAGC STR AAACGATCG STR AAAAA TTCGCCCAA Calculated thermodynamical parameters for dimer: dS = -270.157 dH = -84300 dG = -510.892 t = 2.05342 SEQ AACC C T AAAAA --------- SEQ CG T GCTAGCA AA STR GC A CGATCGT TT STR - T ----- CGCCCAAAA Calculated thermodynamical parameters for dimer: 22 dS = -77.7449 dH = -24700 dG = -587.432 t = 44.5559 SEQ -------///-----\\\---- STR AAAACCCGCTTTGCTAGCTACG Calculated thermodynamical parameters for dimer: dS = -259.714 dH = -84800 dG = -4249.61 t = 13.4561 SEQ AACCCGCTTGCTAGCAAAAAAAAAACCCGCTTGCTAGCAAAAAAAAAACCCGCTTGCTAGCAAAAAAAAAACCCGCTTGCTAGCAAAAAAAAAACC C T ---------- SEQ CG T GCTAGCAA STR GC A CGATCGTT STR - T TCGCCCAAAA No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -34.2024 dH = -10200 dG = 407.884 t = -128.191 SEQ A SEQ AT STR TA STR T Calculated thermodynamical parameters for dimer: dS = -35.8049 dH = -9800 dG = 1304.88 t = -136.976 SEQ SEQ ATA STR TAT STR Calculated thermodynamical parameters for dimer: dS = -34.2024 dH = -10200 dG = 407.884 t = -128.191 SEQ T SEQ AT STR TA STR A Calculated thermodynamical parameters for dimer: dS = -126.11 dH = -43000 dG = -3887.07 t = -8.1626 SEQ A ACACACACACT SEQ AACAC STR TTGTG STR GGTGTTGGGG GGGGT------ Calculated thermodynamical parameters for dimer: dS = -192.202 dH = -61500 dG = -1888.5 t = -3.84345 SEQ AAA C ACACACT------ SEQ CACA AC STR GTGT TG STR G - GGGTTGTGGGGGT Calculated thermodynamical parameters for dimer: dS = -265.564 dH = -84200 dG = -1835.23 t = 5.91073 SEQ AAA -- A C T---- SEQ CACA C CA ACAC STR GTGT G GT TGTG STR G TG G - GGGGT Calculated thermodynamical parameters for dimer: dS = -331.582 dH = -103100 dG = -259.976 t = 7.20829 SEQ AAA C A- - T---- SEQ CACA AC C ACAC STR GTGT TG G TGTG STR G - GG T GGGGT Calculated thermodynamical parameters for dimer: dS = -331.582 dH = -103100 dG = -259.976 t = 7.20829 SEQ AAA C A- - T---- SEQ CACA AC C ACAC STR GTGT TG G TGTG STR G - GG T GGGGT Calculated thermodynamical parameters for dimer: dS = -277.07 dH = -74900 dG = 11033.4 t = -34.0305 SEQ AAAC TC T- CGTTTC T-- SEQ GA G AG AG CT STR CT C TC TC GA STR CT CT TC TC---- TCT Calculated thermodynamical parameters for dimer: dS = -311.393 dH = -82600 dG = 13978.6 t = -35.4899 SEQ A TCT CT-- TCT - TC ---- SEQ G CT C CT C TC STR C GA G GA G AG STR T T-- CTTT C-- T CT CAAA Calculated thermodynamical parameters for dimer: 23 dS = -101.25 dH = -32500 dG = -1097.4 t = 47.8385 SEQ /////-------\\\\\------ STR AAACGATCGTAGCGTTTCAGTCT Calculated thermodynamical parameters for dimer: dS = -28.5 dH = -8000 dG = 839.275 t = -149.43 SEQ ACGCCGTTGCGCGC GCTGCG------------ SEQ T STR A STR TTTTTTTTTTTTTTTTTT Calculated thermodynamical parameters for dimer: 21 dS = -80.1849 dH = -26800 dG = -1930.67 t = 61.0777 SEQ -///----\\\---------- STR ACGCCGTTGCGCGCTGCTGCG Calculated thermodynamical parameters for dimer: dS = -7.1 dH = -800 dG = 1402.06 t = -254.658 SEQ TTTTTTTTTTATTTTTTTT --------- SEQ T STR A STR GGGGGGGGG GGGGGGGGG Calculated thermodynamical parameters for dimer: dS = -22.4 dH = -6000 dG = 947.36 t = -170.695 SEQ TTTTTTTTTTA TTTTTTTT- SEQ T STR A STR GGGGGGGGG GGGGGGGGG Calculated thermodynamical parameters for dimer: dS = -172.412 dH = -61400 dG = -7926.37 t = 21.2295 SEQ AAA ATTTT SEQ CGATCG STR GCTAGC STR CCCCA AAA-- Calculated thermodynamical parameters for dimer: dS = -194.669 dH = -64400 dG = -4023.27 t = 5.84129 SEQ AAA AT SEQ CGATCG TTT STR GCTAGC AAA STR CCCCA -- Calculated thermodynamical parameters for dimer: dS = -186.529 dH = -55000 dG = 2851.92 t = -26.171 SEQ A CG CCC ---- SEQ AA ATCGA C STR TT TAGCT G STR T- A-- CAAA Calculated thermodynamical parameters for dimer: dS = -17.5 dH = -3300 dG = 2127.62 t = -211.654 SEQ AAAAAAAAAAAAAA T------------ SEQ A STR T STR CCCCCCCCCCCCC No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -17.5 dH = -3300 dG = 2127.62 t = -211.654 SEQ CCCCCCCCCCCCC -------------- SEQ T STR A STR T AAAAAAAAAAAAAA Calculated thermodynamical parameters for dimer: dS = -11.5 dH = -1900 dG = 1666.72 t = -233.286 SEQ AAAAAAAAAAAAAA T SEQ A STR T STR CCCCCCCCCCCCC - Calculated thermodynamical parameters for dimer: dS = -297.689 dH = -97000 dG = -4671.71 t = 17.3985 SEQ A ---- -- A SEQ CACACA CAC ACAC STR GTGTGT GTG TGTG STR TGTT GT T Calculated thermodynamical parameters for dimer: dS = -284.292 dH = -92500 dG = -4326.97 t = 15.5032 SEQ A ---- -- SEQ CACACA CAC ACACA STR GTGTGT GTG TGTGT STR TGTT GT Calculated thermodynamical parameters for dimer: dS = -335.807 dH = -108300 dG = -4149.54 t = 18.0034 SEQ TGTGTTG T - - SEQ GTG TGT TGTGTG STR CAC ACA ACACAC STR A - C A Calculated thermodynamical parameters for dimer: dS = -31.6 dH = -8600 dG = 1200.74 t = -146.235 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGGG GGGGGGG------ Calculated thermodynamical parameters for dimer: dS = -12.7 dH = -2000 dG = 1938.9 t = -232.219 SEQ TAAAAAAAAAAAAA ------- SEQ A STR T STR GGGGGGGGG GGGGGGG Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ T AAAAAAAAAAAAA- SEQ A STR T STR GGGGGGGGGGGGGG Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ GGGGGGGGGGGGGG - SEQ T STR A STR AAAAAAAAAAAAA T Calculated thermodynamical parameters for dimer: dS = -16.6 dH = -4400 dG = 748.49 t = -189.757 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGGGGGGGGG ------------- No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -16.6 dH = -4400 dG = 748.49 t = -189.757 SEQ AAAAAAAT AAAAAA SEQ A STR T STR GGGGGGGGGGGGGGG ------ No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ AAAAAAAT AAAAAA-------- SEQ A STR T STR GGGGGGGGGGGGGG No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ GGGGGGGGGGGGGG -------- SEQ T STR A STR AAAAAA TAAAAAAA Calculated thermodynamical parameters for dimer: dS = -25.3 dH = -5800 dG = 2046.79 t = -178.783 SEQ AAAAAAAAAAAAA T----- SEQ A STR T STR GGGGGGGG GGGGGG Calculated thermodynamical parameters for dimer: dS = -31.6 dH = -8600 dG = 1200.74 t = -146.235 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGG GGGGGG------- Calculated thermodynamical parameters for dimer: dS = -12.7 dH = -2000 dG = 1938.9 t = -232.219 SEQ TAAAAAAAAAAAAA ------ SEQ A STR T STR GGGGGGGG GGGGGG Calculated thermodynamical parameters for dimer: dS = -229.719 dH = -79000 dG = -7752.52 t = 23.9748 SEQ AAAACCCGC TACG----- SEQ TTTGCTAGC STR AAACGATCG STR AAAAA TTCGCCCAA Calculated thermodynamical parameters for dimer: dS = -296.617 dH = -97100 dG = -5104.32 t = 18.6354 SEQ AAAACCCGC T - ---- SEQ TTTGCTAGC A CG STR AAACGATCG T GC STR AAAAA T C CCAA Calculated thermodynamical parameters for dimer: dS = -242.857 dH = -72000 dG = 3322.01 t = -15.1029 SEQ AACC C T AAA AA --------- SEQ CG T GCTAGCA A A STR GC A CGATCGT T T STR - T --- -- CGCCCAAAA Calculated thermodynamical parameters for dimer: 22 dS = -77.7449 dH = -24700 dG = -587.432 t = 44.5559 SEQ -------///-----\\\---- STR AAAACCCGCTTTGCTAGCTACG Calculated thermodynamical parameters for dimer: 23 dS = -73.6849 dH = -23400 dG = -546.641 t = 44.4186 SEQ -----///----\\\-------- STR AACCCGCTTGCTAGCAAAAAAAA Calculated thermodynamical parameters for dimer: dS = -331.582 dH = -103100 dG = -259.976 t = 7.20829 SEQ AAA C A- - T---- SEQ CACA AC C ACAC STR GTGT TG G TGTG STR G - GG T GGGGT Calculated thermodynamical parameters for dimer: dS = -330.972 dH = -99300 dG = 3350.83 t = -2.67634 SEQ TGGGG T GG T - --- SEQ GTG TG G TGTG G STR CAC AC C ACAC C STR T - A- - A AAA Calculated thermodynamical parameters for dimer: dS = -147.239 dH = -40300 dG = 5366.31 t = -53.4136 SEQ AAAC TC T- C TTTCAGTCT SEQ GA G AG G STR CT C TC C STR CT -- TC T TCTCTCTGA Calculated thermodynamical parameters for dimer: dS = -63.8049 dH = -18700 dG = 1089.08 t = -86.0883 SEQ AAACGATCGTAGCGTTTCA T SEQ G CT STR C GA STR CTCTCTCTCTCTCTCT T Calculated thermodynamical parameters for dimer: dS = -132.239 dH = -35100 dG = 5914.06 t = -64.7196 SEQ AGTCTCTCTCT T CT -- ---- SEQ C CT C TC STR G GA G AG STR TCTGACTTT C T- CT CAAA Calculated thermodynamical parameters for dimer: 23 dS = -101.25 dH = -32500 dG = -1097.4 t = 47.8385 SEQ /////-------\\\\\------ STR AAACGATCGTAGCGTTTCAGTCT Calculated thermodynamical parameters for dimer: dS = -28.5 dH = -8000 dG = 839.275 t = -149.43 SEQ ACGCCGTTGCGCGC GCTGCG------------ SEQ T STR A STR TTTTTTTTTTTTTTTTTT Calculated thermodynamical parameters for dimer: dS = -28.5 dH = -8000 dG = 839.275 t = -149.43 SEQ TTTTTTTTTTTTTTTTTT ----------------- SEQ A STR T STR GCG CGTCGCGCGTTGCCGCA Calculated thermodynamical parameters for dimer: 21 dS = -80.1849 dH = -26800 dG = -1930.67 t = 61.0777 SEQ -///----\\\---------- STR ACGCCGTTGCGCGCTGCTGCG Calculated thermodynamical parameters for dimer: dS = -22.4 dH = -6000 dG = 947.36 t = -170.695 SEQ TTTTTTTTTTA TTTTTTTT SEQ T STR A STR GGGGGGGGG GGGGGGGG Calculated thermodynamical parameters for dimer: dS = -7.1 dH = -800 dG = 1402.06 t = -254.658 SEQ TTTTTTTTTTATTTTTTTT -------- SEQ T STR A STR GGGGGGGGG GGGGGGGG Calculated thermodynamical parameters for dimer: dS = -321.122 dH = -105100 dG = -5504.14 t = 21.014 SEQ AA G C CC-- SEQ CCGG T TTTTTTTAA STR GGCC A AAAAAAATT STR A - CCAA Calculated thermodynamical parameters for dimer: dS = -55.7024 dH = -18100 dG = -823.891 t = -76.1208 SEQ AACCGGGTCTTTTTTTAA ----------------- SEQ CC STR GG STR CCAAAAAAAAATTCCAA Calculated thermodynamical parameters for dimer: dS = -298.019 dH = -98100 dG = -5669.36 t = 20.4032 SEQ AACCT - A -- SEQ TAAAAAAA A CCGG STR ATTTTTTT T GGCC STR CCA C G AA Calculated thermodynamical parameters for dimer: dS = -172.412 dH = -61400 dG = -7926.37 t = 21.2295 SEQ AAA ATTTT SEQ CGATCG STR GCTAGC STR CCCCA AAA-- Calculated thermodynamical parameters for dimer: dS = -194.669 dH = -64400 dG = -4023.27 t = 5.84129 SEQ AAA AT SEQ CGATCG TTT STR GCTAGC AAA STR CCCCA -- Calculated thermodynamical parameters for dimer: dS = -34.1 dH = -10300 dG = 276.115 t = -126.556 SEQ AAACGATCGACCC -------- SEQ C STR G STR TTTTA CTAGCAAA Calculated thermodynamical parameters for dimer: dS = -17.5 dH = -3300 dG = 2127.62 t = -211.654 SEQ AAAAAAAAAAAAAA T------------ SEQ A STR T STR CCCCCCCCCCCCC No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -17.5 dH = -3300 dG = 2127.62 t = -211.654 SEQ CCCCCCCCCCCCC -------------- SEQ T STR A STR T AAAAAAAAAAAAAA Calculated thermodynamical parameters for dimer: dS = -11.5 dH = -1900 dG = 1666.72 t = -233.286 SEQ AAAAAAAAAAAAAA T SEQ A STR T STR CCCCCCCCCCCCC - Calculated thermodynamical parameters for dimer: dS = -335.807 dH = -108300 dG = -4149.54 t = 18.0034 SEQ A C - A------ SEQ CACACA AC ACAC STR GTGTGT TG TGTG STR - T GTTGTGT Calculated thermodynamical parameters for dimer: dS = -342.162 dH = -107500 dG = -1378.58 t = 10.9982 SEQ A - - -- SEQ CAC AC ACAC ACACA STR GTG TG TGTG TGTGT STR GT T T GT Calculated thermodynamical parameters for dimer: dS = -335.807 dH = -108300 dG = -4149.54 t = 18.0034 SEQ TGTGTTG T - - SEQ GTG TGT TGTGTG STR CAC ACA ACACAC STR A - C A Calculated thermodynamical parameters for dimer: dS = -31.6 dH = -8600 dG = 1200.74 t = -146.235 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGGG GGGGGGG------ Calculated thermodynamical parameters for dimer: dS = -12.7 dH = -2000 dG = 1938.9 t = -232.219 SEQ TAAAAAAAAAAAAA ------- SEQ A STR T STR GGGGGGGGG GGGGGGG Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ T AAAAAAAAAAAAA- SEQ A STR T STR GGGGGGGGGGGGGG Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ GGGGGGGGGGGGGG - SEQ T STR A STR AAAAAAAAAAAAA T Calculated thermodynamical parameters for dimer: dS = -16.6 dH = -4400 dG = 748.49 t = -189.757 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGGGGGGGGG ------------- No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -16.6 dH = -4400 dG = 748.49 t = -189.757 SEQ AAAAAAAT AAAAAA SEQ A STR T STR GGGGGGGGGGGGGGG ------ No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ AAAAAAAT AAAAAA-------- SEQ A STR T STR GGGGGGGGGGGGGG No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ GGGGGGGGGGGGGG -------- SEQ T STR A STR AAAAAA TAAAAAAA Calculated thermodynamical parameters for dimer: dS = -25.3 dH = -5800 dG = 2046.79 t = -178.783 SEQ AAAAAAAAAAAAA T----- SEQ A STR T STR GGGGGGGG GGGGGG Calculated thermodynamical parameters for dimer: dS = -31.6 dH = -8600 dG = 1200.74 t = -146.235 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGG GGGGGG------- Calculated thermodynamical parameters for dimer: dS = -12.7 dH = -2000 dG = 1938.9 t = -232.219 SEQ TAAAAAAAAAAAAA ------ SEQ A STR T STR GGGGGGGG GGGGGG Calculated thermodynamical parameters for dimer: dS = -229.719 dH = -79000 dG = -7752.52 t = 23.9748 SEQ AAAACCCGC TACG----- SEQ TTTGCTAGC STR AAACGATCG STR AAAAA TTCGCCCAA Calculated thermodynamical parameters for dimer: dS = -151.2 dH = -47100 dG = -205.407 t = -21.7648 SEQ AAAACCCGCTTTGCTA C ---------- SEQ GCTA G STR CGAT C STR AAAAAAAA - GTTCGCCCAA Calculated thermodynamical parameters for dimer: dS = -57.3049 dH = -17700 dG = 73.102 t = -83.7784 SEQ AACCCGCTTGCTAGCAAAAA --------- SEQ AAA STR TTT STR GCATCGATCG CGCCCAAAA Calculated thermodynamical parameters for dimer: 22 dS = -77.7449 dH = -24700 dG = -587.432 t = 44.5559 SEQ -------///-----\\\---- STR AAAACCCGCTTTGCTAGCTACG Calculated thermodynamical parameters for dimer: 23 dS = -73.6849 dH = -23400 dG = -546.641 t = 44.4186 SEQ -----///----\\\-------- STR AACCCGCTTGCTAGCAAAAAAAA Calculated thermodynamical parameters for dimer: dS = -192.202 dH = -61500 dG = -1888.5 t = -3.84345 SEQ AAA C ACACACT------ SEQ CACA AC STR GTGT TG STR G - GGGTTGTGGGGGT Calculated thermodynamical parameters for dimer: dS = -213.395 dH = -64900 dG = 1284.33 t = -13.0889 SEQ TGGGGGTGTTGGG - - ------- SEQ GT TGTG G STR CA ACAC C STR T C A ACACAAA Calculated thermodynamical parameters for dimer: dS = -149.687 dH = -41700 dG = 4725.51 t = -48.7748 SEQ AAACGATC T - GTTTCAGTCT SEQ G AG C STR C TC G STR CTCTCTCTCTCTCT - T A--------- Calculated thermodynamical parameters for dimer: dS = -44.9024 dH = -13800 dG = 126.489 t = -102.915 SEQ AAACGATCGTAGCGTTTCAGT SEQ CT STR GA STR CTCTCTCTCTCTCTCTCT Calculated thermodynamical parameters for dimer: dS = -89.3949 dH = -24700 dG = 3025.82 t = -76.4266 SEQ AGTCTCTCTCTCTCTCT - -------- SEQ CT C STR GA G STR TCTGACTTTGC T CTAGCAAA Calculated thermodynamical parameters for dimer: 23 dS = -101.25 dH = -32500 dG = -1097.4 t = 47.8385 SEQ /////-------\\\\\------ STR AAACGATCGTAGCGTTTCAGTCT Calculated thermodynamical parameters for dimer: dS = -28.5 dH = -8000 dG = 839.275 t = -149.43 SEQ ACGCCGTTGCGCGC GCTGCG------------ SEQ T STR A STR TTTTTTTTTTTTTTTTTT Calculated thermodynamical parameters for dimer: dS = -28.5 dH = -8000 dG = 839.275 t = -149.43 SEQ TTTTTTTTTTTTTTTTTT ----------------- SEQ A STR T STR GCG CGTCGCGCGTTGCCGCA Calculated thermodynamical parameters for dimer: 21 dS = -80.1849 dH = -26800 dG = -1930.67 t = 61.0777 SEQ -///----\\\---------- STR ACGCCGTTGCGCGCTGCTGCG Calculated thermodynamical parameters for dimer: dS = -22.4 dH = -6000 dG = 947.36 t = -170.695 SEQ TTTTTTTTTTA TTTTTTTT SEQ T STR A STR GGGGGGGGG GGGGGGGG Calculated thermodynamical parameters for dimer: dS = -7.1 dH = -800 dG = 1402.06 t = -254.658 SEQ TTTTTTTTTTATTTTTTTT -------- SEQ T STR A STR GGGGGGGGG GGGGGGGG Calculated thermodynamical parameters for dimer: dS = -304.907 dH = -96700 dG = -2133.18 t = 10.3705 SEQ AA G C AACC--- SEQ CC GGT TTTTTTT STR GG CCA AAAAAAA STR - - ATTCCAA Calculated thermodynamical parameters for dimer: dS = -55.7024 dH = -18100 dG = -823.891 t = -76.1208 SEQ AACCGGGTCTTTTTTTAA ----------------- SEQ CC STR GG STR CCAAAAAAAAATTCCAA Calculated thermodynamical parameters for dimer: dS = -347.099 dH = -106400 dG = 1252.8 t = 4.46739 SEQ AACCT A - - -- SEQ T AAAAAAA A CCGG STR A TTTTTTT T GGCC STR CCA - C G AA Calculated thermodynamical parameters for dimer: dS = -172.412 dH = -61400 dG = -7926.37 t = 21.2295 SEQ AAA ATTTT SEQ CGATCG STR GCTAGC STR CCCCA AAA-- Calculated thermodynamical parameters for dimer: dS = -240.399 dH = -75000 dG = -440.115 t = -1.96266 SEQ AAA A T SEQ CGATCG T TT STR GCTAGC A AA STR CCCCA - - Calculated thermodynamical parameters for dimer: dS = -34.1 dH = -10300 dG = 276.115 t = -126.556 SEQ AAACGATCGACCC -------- SEQ C STR G STR TTTTA CTAGCAAA Calculated thermodynamical parameters for dimer: dS = -17.5 dH = -3300 dG = 2127.62 t = -211.654 SEQ AAAAAAAAAAAAAA T------------ SEQ A STR T STR CCCCCCCCCCCCC No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -17.5 dH = -3300 dG = 2127.62 t = -211.654 SEQ CCCCCCCCCCCCC -------------- SEQ T STR A STR T AAAAAAAAAAAAAA Calculated thermodynamical parameters for dimer: dS = -11.5 dH = -1900 dG = 1666.72 t = -233.286 SEQ AAAAAAAAAAAAAA T SEQ A STR T STR CCCCCCCCCCCCC - Calculated thermodynamical parameters for dimer: dS = -335.807 dH = -108300 dG = -4149.54 t = 18.0034 SEQ A C - A------ SEQ CACACA AC ACAC STR GTGTGT TG TGTG STR - T GTTGTGT Calculated thermodynamical parameters for dimer: dS = -316.307 dH = -100000 dG = -1897.47 t = 10.563 SEQ A C C -------- SEQ CACACA ACA ACA STR GTGTGT TGT TGT STR - - GGTTGTGT Calculated thermodynamical parameters for dimer: dS = -335.807 dH = -108300 dG = -4149.54 t = 18.0034 SEQ TGTGTTG T - - SEQ GTG TGT TGTGTG STR CAC ACA ACACAC STR A - C A Calculated thermodynamical parameters for dimer: dS = -31.6 dH = -8600 dG = 1200.74 t = -146.235 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGGG GGGGGGG------ Calculated thermodynamical parameters for dimer: dS = -12.7 dH = -2000 dG = 1938.9 t = -232.219 SEQ TAAAAAAAAAAAAA ------- SEQ A STR T STR GGGGGGGGG GGGGGGG Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ T AAAAAAAAAAAAA- SEQ A STR T STR GGGGGGGGGGGGGG Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ GGGGGGGGGGGGGG - SEQ T STR A STR AAAAAAAAAAAAA T Calculated thermodynamical parameters for dimer: dS = -16.6 dH = -4400 dG = 748.49 t = -189.757 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGGGGGGGGG ------------- No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -16.6 dH = -4400 dG = 748.49 t = -189.757 SEQ AAAAAAAT AAAAAA SEQ A STR T STR GGGGGGGGGGGGGGG ------ No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ AAAAAAAT AAAAAA-------- SEQ A STR T STR GGGGGGGGGGGGGG No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ GGGGGGGGGGGGGG -------- SEQ T STR A STR AAAAAA TAAAAAAA Calculated thermodynamical parameters for dimer: dS = -25.3 dH = -5800 dG = 2046.79 t = -178.783 SEQ AAAAAAAAAAAAA T----- SEQ A STR T STR GGGGGGGG GGGGGG Calculated thermodynamical parameters for dimer: dS = -31.6 dH = -8600 dG = 1200.74 t = -146.235 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGG GGGGGG------- Calculated thermodynamical parameters for dimer: dS = -12.7 dH = -2000 dG = 1938.9 t = -232.219 SEQ TAAAAAAAAAAAAA ------ SEQ A STR T STR GGGGGGGG GGGGGG Calculated thermodynamical parameters for dimer: dS = -229.719 dH = -79000 dG = -7752.52 t = 23.9748 SEQ AAAACCCGC TACG----- SEQ TTTGCTAGC STR AAACGATCG STR AAAAA TTCGCCCAA Calculated thermodynamical parameters for dimer: dS = -63.8024 dH = -21300 dG = -1511.68 t = -60.0745 SEQ AAAACCCGCTTTGCTAGCTA ---- SEQ CG STR GC STR AAAAAAAACGATCGTTC CCAA Calculated thermodynamical parameters for dimer: dS = -57.3049 dH = -17700 dG = 73.102 t = -83.7784 SEQ AACCCGCTTGCTAGCAAAAA --------- SEQ AAA STR TTT STR GCATCGATCG CGCCCAAAA Calculated thermodynamical parameters for dimer: 22 dS = -77.7449 dH = -24700 dG = -587.432 t = 44.5559 SEQ -------///-----\\\---- STR AAAACCCGCTTTGCTAGCTACG Calculated thermodynamical parameters for dimer: 23 dS = -73.6849 dH = -23400 dG = -546.641 t = 44.4186 SEQ -----///----\\\-------- STR AACCCGCTTGCTAGCAAAAAAAA Calculated thermodynamical parameters for dimer: dS = -126.11 dH = -43000 dG = -3887.07 t = -8.1626 SEQ A ACACACACACT SEQ AACAC STR TTGTG STR GGTGTTGGGG GGGGT------ Calculated thermodynamical parameters for dimer: dS = -50.2 dH = -15900 dG = -330.47 t = -89.0416 SEQ TGGGGGTGTTGGGGTTGTG ------------- SEQ G STR C STR TCA ACACACACACAAA Calculated thermodynamical parameters for dimer: dS = -74.6024 dH = -22900 dG = 237.944 t = -66.4052 SEQ AAAC TCGTAGCGTTTCAGTCT SEQ GA STR CT STR CT CTCTCTCTCTCTCTGA- Calculated thermodynamical parameters for dimer: dS = -44.9024 dH = -13800 dG = 126.489 t = -102.915 SEQ AAACGATCGTAGCGTTTCAGT SEQ CT STR GA STR CTCTCTCTCTCTCTCTCT Calculated thermodynamical parameters for dimer: dS = -59.6024 dH = -17700 dG = 785.694 t = -88.3217 SEQ AGTCTCTCTCTCTCTCTC ---- SEQ TC STR AG STR TCTGACTTTGCGATGCT CAAA Calculated thermodynamical parameters for dimer: 23 dS = -101.25 dH = -32500 dG = -1097.4 t = 47.8385 SEQ /////-------\\\\\------ STR AAACGATCGTAGCGTTTCAGTCT Calculated thermodynamical parameters for dimer: dS = -28.5 dH = -8000 dG = 839.275 t = -149.43 SEQ ACGCCGTTGCGCGC GCTGCG------------ SEQ T STR A STR TTTTTTTTTTTTTTTTTT Calculated thermodynamical parameters for dimer: dS = -28.5 dH = -8000 dG = 839.275 t = -149.43 SEQ TTTTTTTTTTTTTTTTTT ----------------- SEQ A STR T STR GCG CGTCGCGCGTTGCCGCA Calculated thermodynamical parameters for dimer: 21 dS = -80.1849 dH = -26800 dG = -1930.67 t = 61.0777 SEQ -///----\\\---------- STR ACGCCGTTGCGCGCTGCTGCG Calculated thermodynamical parameters for dimer: dS = -22.4 dH = -6000 dG = 947.36 t = -170.695 SEQ TTTTTTTTTTA TTTTTTTT SEQ T STR A STR GGGGGGGGG GGGGGGGG Calculated thermodynamical parameters for dimer: dS = -7.1 dH = -800 dG = 1402.06 t = -254.658 SEQ TTTTTTTTTTATTTTTTTT -------- SEQ T STR A STR GGGGGGGGG GGGGGGGG Calculated thermodynamical parameters for dimer: dS = -204.519 dH = -66600 dG = -3168.3 t = 3.56415 SEQ AACCGGGTC CC-- SEQ TTTTTTTAA STR AAAAAAATT STR GGCCAA CCAA Calculated thermodynamical parameters for dimer: dS = -55.7024 dH = -18100 dG = -823.891 t = -76.1208 SEQ AACCGGGTCTTTTTTTAA ----------------- SEQ CC STR GG STR CCAAAAAAAAATTCCAA Calculated thermodynamical parameters for dimer: dS = -121.407 dH = -42900 dG = -5245.53 t = -0.889075 SEQ AACCTTAAAAAAAAA -- SEQ CCGG STR GGCC STR CCAATTTTTTTCTG AA Calculated thermodynamical parameters for dimer: dS = -172.412 dH = -61400 dG = -7926.37 t = 21.2295 SEQ AAA ATTTT SEQ CGATCG STR GCTAGC STR CCCCA AAA-- Calculated thermodynamical parameters for dimer: dS = -28.4024 dH = -8300 dG = 509.014 t = -144.597 SEQ AAACGATCGATT SEQ TT STR AA STR CCCCAGCTAGCA Calculated thermodynamical parameters for dimer: dS = -34.1 dH = -10300 dG = 276.115 t = -126.556 SEQ AAACGATCGACCC -------- SEQ C STR G STR TTTTA CTAGCAAA Calculated thermodynamical parameters for dimer: dS = -17.5 dH = -3300 dG = 2127.62 t = -211.654 SEQ AAAAAAAAAAAAAA T------------ SEQ A STR T STR CCCCCCCCCCCCC No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -17.5 dH = -3300 dG = 2127.62 t = -211.654 SEQ CCCCCCCCCCCCC -------------- SEQ T STR A STR T AAAAAAAAAAAAAA Calculated thermodynamical parameters for dimer: dS = -11.5 dH = -1900 dG = 1666.72 t = -233.286 SEQ AAAAAAAAAAAAAA T SEQ A STR T STR CCCCCCCCCCCCC - Calculated thermodynamical parameters for dimer: dS = -136.012 dH = -47100 dG = -4915.83 t = 0.410002 SEQ A CACACACA------ SEQ CACACA STR GTGTGT STR TGTTGTGGTTGTGT Calculated thermodynamical parameters for dimer: dS = -134.812 dH = -46400 dG = -4588.01 t = -1.76417 SEQ ACACACACA -------------- SEQ CACACA STR GTGTGT STR TGTTGTGGTTGTGT Calculated thermodynamical parameters for dimer: dS = -147.71 dH = -50700 dG = -4887.83 t = 2.5855 SEQ TGTGTTGGTGTTGTT --------- SEQ GTGTG STR CACAC STR A ACACACACA Calculated thermodynamical parameters for dimer: dS = -31.6 dH = -8600 dG = 1200.74 t = -146.235 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGGG GGGGGGG------ Calculated thermodynamical parameters for dimer: dS = -12.7 dH = -2000 dG = 1938.9 t = -232.219 SEQ TAAAAAAAAAAAAA ------- SEQ A STR T STR GGGGGGGGG GGGGGGG Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ T AAAAAAAAAAAAA- SEQ A STR T STR GGGGGGGGGGGGGG Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ GGGGGGGGGGGGGG - SEQ T STR A STR AAAAAAAAAAAAA T Calculated thermodynamical parameters for dimer: dS = -16.6 dH = -4400 dG = 748.49 t = -189.757 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGGGGGGGGG ------------- No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -16.6 dH = -4400 dG = 748.49 t = -189.757 SEQ AAAAAAAT AAAAAA SEQ A STR T STR GGGGGGGGGGGGGGG ------ No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ AAAAAAAT AAAAAA-------- SEQ A STR T STR GGGGGGGGGGGGGG No predicted secondary structures for given sequences Calculated thermodynamical parameters for dimer: dS = -28 dH = -7000 dG = 1684.2 t = -164.051 SEQ GGGGGGGGGGGGGG -------- SEQ T STR A STR AAAAAA TAAAAAAA Calculated thermodynamical parameters for dimer: dS = -25.3 dH = -5800 dG = 2046.79 t = -178.783 SEQ AAAAAAAAAAAAA T----- SEQ A STR T STR GGGGGGGG GGGGGG Calculated thermodynamical parameters for dimer: dS = -31.6 dH = -8600 dG = 1200.74 t = -146.235 SEQ T AAAAAAAAAAAAA SEQ A STR T STR GGGGGGGG GGGGGG------- Calculated thermodynamical parameters for dimer: dS = -12.7 dH = -2000 dG = 1938.9 t = -232.219 SEQ TAAAAAAAAAAAAA ------ SEQ A STR T STR GGGGGGGG GGGGGG Calculated thermodynamical parameters for dimer: dS = -336.826 dH = -112500 dG = -8033.28 t = 28.4678 SEQ A C AGC-------- SEQ AAG T AGCTAGCTAGCT STR TTC A TCGATCGATCGA STR TTTTTT G - CTAGAATTTTT Calculated thermodynamical parameters for dimer: dS = -412.309 dH = -135600 dG = -7722.5 t = 29.2109 SEQ G TC ------------ SEQ AA A AGCTAGCTAGCTAGC STR TT T TCGATCGATCGATCG STR TTT - T- ACTAGAATTTTT Calculated thermodynamical parameters for dimer: dS = -383.796 dH = -128500 dG = -9465.63 t = 32.8328 SEQ TTTTTAAGATCA TTTTT SEQ GCTAGCTAGCTAGCT TT STR CGATCGATCGATCGA AA STR CTAG- Calculated thermodynamical parameters for dimer: 21 dS = -84.9873 dH = -27600 dG = -1241.19 t = 51.6044 SEQ ------////----\\\\--- STR AAGATCAGCTAGCTAGCTAGC Calculated thermodynamical parameters for dimer: 34 dS = -127.492 dH = -42300 dG = -2758.31 t = 58.6351 SEQ -----------//////----\\\\\\------- STR TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT Calculated thermodynamical parameters for dimer: dS = -184.797 dH = -60800 dG = -3485.3 t = 2.01435 SEQ G TC TTTT------ SEQ A AGCTAGCT STR T TCGATCGA STR TT T- CTAGTTTTTT Calculated thermodynamical parameters for dimer: dS = -207.856 dH = -64300 dG = 166.667 t = -9.64545 SEQ G TC TTT ------- SEQ A AGCTAGCT T STR T TCGATCGA A STR TT T- CT- GTTTTTT Calculated thermodynamical parameters for dimer: dS = -207.856 dH = -64300 dG = 166.667 t = -9.64545 SEQ TTTTTTG TC TTT - SEQ A AGCTAGCT T STR T TCGATCGA A STR TT T- CT- G Calculated thermodynamical parameters for dimer: dS = -418.711 dH = -138200 dG = -8336.78 t = 30.671 SEQ G TC TTTT------- SEQ AA A AGCTAGCTAGCTAGCT STR TT T TCGATCGATCGATCGA STR TT - T- CTAGCCTTTTT Calculated thermodynamical parameters for dimer: dS = -441.771 dH = -141700 dG = -4684.81 t = 23.3352 SEQ G TC TTT -------- SEQ AA A AGCTAGCTAGCTAGCT T STR TT T TCGATCGATCGATCGA A STR TT - T- CT- GCCTTTTT Calculated thermodynamical parameters for dimer: dS = -417.023 dH = -136600 dG = -7260.18 t = 28.2718 SEQ TTTTTCCG TC TT T SEQ A AGCTAGCTAGCTAGCT T TT STR T TCGATCGATCGATCGA A AA STR TT T- CT G Calculated thermodynamical parameters for dimer: 26 dS = -127.492 dH = -42300 dG = -2758.31 t = 58.6351 SEQ ------//////----\\\\\\---- STR AAGATCAGCTAGCTAGCTAGCTTTTT Calculated thermodynamical parameters for dimer: 33 dS = -127.492 dH = -42300 dG = -2758.31 t = 58.6351 SEQ -----------//////----\\\\\\------ STR TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT Calculated thermodynamical parameters for dimer: dS = -235.376 dH = -68200 dG = 4801.99 t = -21.9886 SEQ CGA TTT- TC- --- SEQ TATA GG TATA STR ATAT CC ATAT STR CTGG TTT AGC Calculated thermodynamical parameters for dimer: dS = -235.376 dH = -68200 dG = 4801.99 t = -21.9886 SEQ CGA TTT- TC- --- SEQ TATA GG TATA STR ATAT CC ATAT STR CTGG TTT AGC Calculated thermodynamical parameters for dimer: dS = -235.376 dH = -68200 dG = 4801.99 t = -21.9886 SEQ CGA TTT GGTC --- SEQ TATA CC TATA STR ATAT GG ATAT STR CT- TTT- AGC Calculated thermodynamical parameters for dimer: dS = -225.455 dH = -68200 dG = 1724.73 t = -12.4633 SEQ CGA TTT- TC- --- SEQ TATA GG TATA STR ATAT CC ATAT STR CTGG TTT AGC Calculated thermodynamical parameters for dimer: dS = -224.973 dH = -68200 dG = 1575.41 t = -11.9827 SEQ CGA TTT- TC- --- SEQ TATA GG TATA STR ATAT CC ATAT STR CTGG TTT AGC Calculated thermodynamical parameters for dimer: dS = -230.68 dH = -68200 dG = 3345.31 t = -17.5679 SEQ CGA TTT- TC- --- SEQ TATA GG TATA STR ATAT CC ATAT STR CTGG TTT AGC Calculated thermodynamical parameters for dimer: dS = -230.68 dH = -68200 dG = 3345.31 t = -17.5679 SEQ CGA TTT- TC- --- SEQ TATA GG TATA STR ATAT CC ATAT STR CTGG TTT AGC Calculated thermodynamical parameters for dimer: dS = -161.607 dH = -49500 dG = 622.413 t = -21.1027 SEQ --- TTGGTCTATA SEQ CG ATATAT STR GC TATATA STR ATATCTG CTT GC-------- Calculated thermodynamical parameters for dimer: dS = -161.607 dH = -49500 dG = 622.413 t = -15.0902 SEQ --- TTGGTCTATA SEQ CG ATATAT STR GC TATATA STR ATATCTG CTT GC-------- Calculated thermodynamical parameters for dimer: dS = -153.89 dH = -49500 dG = -1771.02 t = -4.27297 SEQ --- TTGGTCTATA SEQ CG ATATAT STR GC TATATA STR ATATCTG CTT GC-------- Calculated thermodynamical parameters for dimer: dS = -235.376 dH = -68200 dG = -1553.17 t = -21.9886 SEQ CGA TTT- TC- --- SEQ TATA GG TATA STR ATAT CC ATAT STR CTGG TTT AGC Calculated thermodynamical parameters for dimer: dS = -235.376 dH = -68200 dG = 4801.99 t = -21.9886 SEQ CGA TTT- TC- --- SEQ TATA GG TATA STR ATAT CC ATAT STR CTGG TTT AGC Calculated thermodynamical parameters for dimer: dS = -235.376 dH = -68200 dG = 9038.77 t = -21.9886 SEQ CGA TTT- TC- --- SEQ TATA GG TATA STR ATAT CC ATAT STR CTGG TTT AGC Calculated thermodynamical parameters for dimer: dS = -235.376 dH = -68200 dG = 19630.7 t = -21.9886 SEQ CGA TTT- TC- --- SEQ TATA GG TATA STR ATAT CC ATAT STR CTGG TTT AGC Calculated thermodynamical parameters for dimer: dS = -186.517 dH = -64000 dG = -6151.75 t = 14.259 SEQ GT SEQ GTGCAAAA STR CACGTTTT STR C Calculated thermodynamical parameters for dimer: dS = -170.579 dH = -51200 dG = 1705.21 t = -25.4978 SEQ AGT T GT ----- SEQ G A ACGG STR C T TGCC STR - G- AAAAC Calculated thermodynamical parameters for dimer: dS = -165.067 dH = -51200 dG = -4.385 t = -18.714 SEQ AGT T GT ----- SEQ G A ACGG STR C T TGCC STR - G- AAAAC Calculated thermodynamical parameters for dimer: dS = -112.307 dH = -39400 dG = -4567.89 t = -7.77551 SEQ AGTGTAGT ----- SEQ ACGG STR TGCC STR CTG AAAAC Calculated thermodynamical parameters for dimer: dS = -170.579 dH = -51200 dG = 1705.21 t = -25.4978 SEQ AGT T GT ----- SEQ G A ACGG STR C T TGCC STR - G- AAAAC Calculated thermodynamical parameters for dimer: dS = -170.579 dH = -51200 dG = 1705.21 t = -25.4978 SEQ AGT T GT ----- SEQ G A ACGG STR C T TGCC STR - G- AAAAC Calculated thermodynamical parameters for dimer: dS = -149.687 dH = -48100 dG = -1674.58 t = -14.3379 SEQ CAAAA GT ------ SEQ CCGT C STR GGCA G STR T- ATGTGA Calculated thermodynamical parameters for dimer: 29 dS = -240.662 dH = -81400 dG = -6758.72 t = 65.0839 SEQ ///////////-------\\\\\\\\\\\ STR CCAAAACCCATGATGATGATGGGTTTTGG Calculated thermodynamical parameters for dimer: 33 dS = -282.667 dH = -97400 dG = -9730.92 t = 71.4254 SEQ /////////////-------\\\\\\\\\\\\\ STR CCCCAAAACCCATGATGATGATGGGTTTTGGGG primer3-2.3.6/test/primer3web_v3_0_0_default_settings_input0000664000077300007730000000304612231156245022753 0ustar ioanaioanaSEQUENCE_ID=test_settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag = primer3-2.3.6/test/cmd_test3_output0000664000077300007730000000424512231156245016272 0ustar ioanaioana Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 2009,2010,2011,2012 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of the primer3 suite and libraries. The primer3 suite and libraries are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this software (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. USAGE: ../src/primer3_core [-format_output] [-default_version=1|-default_version=2] [-io_version=4] [-p3_settings_file=] [-echo_settings_file] [-strict_tags] [-output=] [-error=] [input_file] This is primer3 (libprimer3 release 2.3.5) Input can also be provided on standard input. For example: $ primer3_core < my_input_file primer3-2.3.6/test/repeat_lib10000664000077300007730000000104212231156245015144 0ustar ioanaioanaATCGTACTAGCTAGCAGCTGATCGATGCTAGCGATCGTACTATCATCGTAGCTACTATCGATCTA AGCTAGCTAGTCGATCTACTAGTCGATCGATCTAGCTACTAGTCGATCGAGCTACTATCATCTAC >seq2 GATCATCTAGCTAGCTGATCGTACTATCATCTAGCTACTATCTACTACTATCTACTATCATCTAG ATCGTACTAGCTAGCAGCTGATCGATGCTAGCGATCGTACTATCATCGTAGCTACTATCGATCTA AGCTAGCTAGTCGATCTACTAGTCGATCGATCTAGCTACTAGTCGATCGAGCTACTATCATCTAC >seq3 GATCATCTAGCTAGCTGATCGTACTATCATCTAGCTACTATCTACTACTATCTACTATCATCTAG ATCGTACTAGCTAGCAGCTGATCGATGCTAGCGATCGTACTATCATCGTAGCTACTATCGATCTA AGCTAGCTAGTCGATCTACTAGTCGATCGATCTAGCTACTAGTCGATCGAGCTACTATCATCTAC primer3-2.3.6/test/primer1_th_list_tmp/0000775000077300007730000000000012231156372017023 5ustar ioanaioanaprimer3-2.3.6/test/vgrep.pl0000775000077300007730000000070512231156245014522 0ustar ioanaioana#!/usr/local/bin/perl system "grep ERROR *.vg */*.vg | grep -v 'ERROR SUMMARY: 0 errors'"; system "grep 'definitely lost' *.vg */*.vg | grep -v ' 0 bytes'"; system "grep 'possibly lost' *.vg */*.vg | grep -v ' 0 bytes'"; system "grep ERROR *.valg* | grep -v 'ERROR SUMMARY: 0 errors'"; system "grep 'definitely lost' *.valg* | grep -v ' 0 bytes'"; system "grep 'possibly lost' *.valg* | grep -v ' 0 bytes'"; primer3-2.3.6/test/primer_human_input0000664000077300007730000051170212231156245016673 0ustar ioanaioanaPRIMER_OPT_SIZE=20 PRIMER_PICK_INTERNAL_OLIGO=1 P3_COMMENT=Used to have: PRIMER_INTERNAL_OLIGO_OFFSET 4 (not used) PRIMER_INTERNAL_MAX_SELF_END=8 PRIMER_MIN_SIZE=18 PRIMER_MAX_SIZE=25 PRIMER_OPT_TM=60.0 PRIMER_MIN_TM=57.0 PRIMER_MAX_TM=63.0 PRIMER_MIN_GC=20.0 PRIMER_MAX_GC=80.0 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_SALT_MONOVALENT=50.0 PRIMER_DNA_CONC=50.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=8 P3_COMMENT= Used to have: PRIMER_NUM_TERMINATORS_0 (not used) P3_COMMENT= Used to have: PRIMER_REST_SITES_" " (not used) P3_COMMENT= Used to have: PRIMER_ALLOW_REST_SITE_0 (not used) PRIMER_PRODUCT_SIZE_RANGE=75-100 100-125 125-150 150-175 175-200 200-225 SEQUENCE_ID=MH1000 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTNAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG SEQUENCE_INCLUDED_REGION=36,342 P3_COMMENT=CL is TTACTGGAAGAGAGGGTCATG P3_COMMENT=CR is AGGGAAACATCTGCAAGGTT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH101 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGATCATCTTCTGATCCAAGACCCGTGCTAGTNCTACTGTATTCTACATGTACCCTTCTACAAGCTTTGAATTCCATAATCATCAGAGAATCCAGAAATTATTCATTATCACATAATAACAACACTCAAATATTTAAAGTTTTATGGTTGAATCTTTATGATTGAACCCATTCTATCAGGTGGTTATTTTATATCTAATAGAATTCATTTCAGGGTTACACAAAGGTCCGAATCTATCCATTTTACTGAG SEQUENCE_INCLUDED_REGION=35,254 P3_COMMENT=CL is CCTCTGATCATCTTCTGATCC P3_COMMENT=CR is GTAAAATGGATAGATTCGGACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1013 SEQUENCE_TEMPLATE=CTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGTGGAGAAGCCTGGTCAGGGAGAGTATACAGGGATGGGGACTGCATGTATGGTTTGGAGTNCAGGAGGGAGAAAGGTGGTTCTTGTGGGAAGAGGTCATCTTCTAGGGCTGAGTTTGGCATTCTTAAGTTTTAACTCTGGGCCTGATATTCCCTGTTAGCCATCTCCAGGAAGAAGCATTTCATGACTCATATTTTTAACATGACTAATGTTACCTAGAACCACGTATCCTCC SEQUENCE_INCLUDED_REGION=33,235 P3_COMMENT=CL is CTGGTCAGGGAGAGTATACAGG P3_COMMENT=CR is GTGGTTCTAGGTAACATTAGTCATG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1017 SEQUENCE_TEMPLATE=TGTTTCATGCTGCAGGTCGACTCTAGAGGATCCCTACATAAACAGCAGCGAAAGGAGAAAGCTTTAGGGCAGAAGCAGGACTGGGCCTGTGGNATCTATAGGTAAGATGAATCCCTTTGGGGAAGACCTTGAATAAGAGATTGAAAGGAAGAGTGGGGTCTATCATTTGCAAAG SEQUENCE_INCLUDED_REGION=34,140 P3_COMMENT=CL is ACATAAACAGCAGCGAAAGG P3_COMMENT=CR is TGCAAATGATAGACCCCACT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1020 SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTCCCAAGATCACAGGTCAAGACTTCTACTATCAGGAGACTCATGTCTTTGAATATTTTTCTTCTTTATGCCTCTATGAACAATAGAAATGAAAAGGGGATCTGTTGTATACACTCACAAGGTATACTTTTATTTGTGAAGGATTTTTCAG SEQUENCE_INCLUDED_REGION=33,154 P3_COMMENT=CL is TTTTCCCAAGATCACAGGTC P3_COMMENT=CR is CCTTCACAAATAAAAGTATACCTTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1022 SEQUENCE_TEMPLATE=GCTTGCATGCTNCAGGTCGACTCTAGAGGATCCCCTTTTTAAAAAAATATTTTCCCATGATTAAACACTTCTAGTGAACTTATAAAGGCCACCACTTCTCTCTCGTTCCTGCTCCTACACCACCACCTNCCCCTCAACTTGTTTTCCTCCTCCTTCACCTCCTGAATCAGCCAGCATAGAGTTTAGTTTATATAAACTTATTCAACCAGGGAAAATTTGGCAGGGATGTCATACTGATTGTTCTCACCTGTGGTAAAGGATTTGTGGATTGAGTCCTCTACTTAGGTACCATTTAATGATTAACCCCAAAGTGGGCAAAGAACCAGAAACCTGGAAG SEQUENCE_INCLUDED_REGION=35,302 P3_COMMENT=CL is AAAGGCCACCACTTCTCTCT P3_COMMENT=CR is TCCAGGTTTCTGGTTCTTTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1026 SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTAAGCACTGAATGAAAATTAATTGTTAAATTCTGAGAATTGAACCTTCTTGTATTTTAATTTTGTTCAAACGTATTTTTTTGTTTTTCTTTTTTTTGTAAAAAAAAGAAAAAGCACAAACTTTCTTCTGCCTGCAACATTTCAGTAGGGTNNAACATCCTTTCTTTGTATTTGAATTCACTTTTTTGATGCAAGAAGAAAGGGAAAGATCTTGATGCAGGAAAATGGCATTGGCTCACAGATGCTATCTGCCATGCTTTTGTGCAG SEQUENCE_INCLUDED_REGION=34,267 P3_COMMENT=CL is ATTCTGAGAATTGAACCTTCTTG P3_COMMENT=CR is ACAAAAGCATGGCAGATAGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1027 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCTCTTATTCCATTCTGCCTCTCTCAGGGTGTTCTCCTCTGCCAAAGTGGGGGGACCCTCTTCTGATGTTTACGCGAGTCTCAAGTCCCCAGGGATTCATCTCACTGTGATTTAGCCAGGGCCTTGTTGCTTCTTCGGGAATCAGATGGCTGGACCACGAAAGCCCAGCGCCTTTCCTATCCTGAAAGAAG SEQUENCE_INCLUDED_REGION=36,192 P3_COMMENT=CL is GGGACCCTCTTCTGATGTTT P3_COMMENT=CR is TTCTTTCAGGATAGGAAAGGC P3_COMMENT=CM is CGCGAGTCTCAAGTCCCC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1033 SEQUENCE_TEMPLATE=ANTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGGAGCCAAAGCCACACCTGAACCCCTTTAAACCATACCTGGGGTGGTCAAGGAGTCTGCATCAGAATGTGGGAAGCAGAGACTTGAGGCAGCCCTGGGAAGTAAACCCCAAGATCTTCAGCGCACCCTAGGCTCTCTTTTGCAGGATTTCCTTCCCTCAGATCATGACACTCTGGGCCTATGACGGGAGGNGCTACGCAGTAATCTNCAAAGTACCTTTGAGGTTATTCTCCCATTGTCCTAATTAGTAG SEQUENCE_INCLUDED_REGION=33,252 P3_COMMENT=CL is CCTGAACCCCTTTAAACCAT P3_COMMENT=CR is ATGGGAGAATAACCTCAAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1034 SEQUENCE_TEMPLATE=GCATGNCTNCAGGTCGACTCTAGAGGATCCCCTTCCTGGTTGGCAAGACTTCATAAATTTTGCCATTATTTGAAGATAACCCACACCTTGGTGTCCAGGGTTTTCACAGGTATTAGTGGTCAGTCACATAGGCATATAGTACCTGTATGACTTCTATTCCAGCCACCGCAAACTTCTCCTCTCCCTGCTGGCTCCTGAGCCAAAAACAGGCATTTACCATAAATCACTTTGTTAGGATGAACTTATCTGGCCAAACTGATACGGCATGACCCACAGCCTCAGGTATATAAAACACTCTCATCAGGCAGAATGTTTCAGGGTCTCAGAGGTTAGTCAGGGGCTAGTCAAGGACCAATCCTGAAGGCAGGCC SEQUENCE_INCLUDED_REGION=32,338 P3_COMMENT=CL is TTTGCCATTATTTGAAGATAACC P3_COMMENT=CR is GGATTGGTCCTTGACTAGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1036 SEQUENCE_TEMPLATE=AGACAGGAGGAAGGCAGCAGAATGGTCTGACAAAGATGTCTGCAGGCAGGAGGGTCTGCAGAATTCATACCTAATTCCCTCAGTTTTCTTGGTGAACCAGGGGGTGGTGACTTGTGCTAGGCACCCAAACTAGAGACCAATCACTCCTTTTCACAAACATTTTATAAAGAATAGTCTTGTTCTCTTCTAATGAAGAAAGAGTTTTCTCTGCTCTTTTAGTTAGGCTGCTGAGCAC SEQUENCE_INCLUDED_REGION=0,235 P3_COMMENT=CL is CAGCAGAATGGTCTGACAAA P3_COMMENT=CR is TGCTCAGCAGCCTAACTAAA P3_COMMENT=CM is ATGTCTGCAGGCAGGAGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1040 SEQUENCE_TEMPLATE=CNNCNNCCTCCAGGTCGACTCTAGAGGATCCCCCTTCACAAAAAGATAAGCAAAGAAGGAATGATATTAAAACTCTTTTACCTGGAGAAGAGGGTTGCATTCCNTTAGACTGTATAATGTGGCTTCAGACAGTGATGCAGGGGGAAGTTATATGCCTTCCTGGGAACTCATGATTAGAGAAGTCAGTAGAAGAAAACAAAGTTGAGAGTGAGGCCAAGACACGACCAGAGAAAAATGAGAGAGTTGTTCATTGAATGAGTTAAGTCAAAGAAAATGGAAGGAAAATTATGAGGTCTGTGGGCTATCTAATGCTCCTATTATGAAGTGTGGTTGAGTTGGAGG SEQUENCE_INCLUDED_REGION=33,309 P3_COMMENT=CL is CACAAAAAGATAAGCAAAGAAGG P3_COMMENT=CR is TCCAACTCAACCACACTTCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1041 SEQUENCE_TEMPLATE=GCTNCNNGCCTCCNGGTCGACTCTAGAGGATCCCTGTCCCAAATCAATCTTACCAGAACAATCTCAAAANANATGTTTTCTCTTCTGATATACATGAACATGGCCCAAAAAACATAAAAAAAAGAAGAAAATACCTCTTACTGGTTGATGAGGACCCCACATACATCCTGAGAATAAGGCACACGTAATGGATTGTGAAACCTCACATGAGAAATAGGTCTGGATGATGTTGTTTGGGCTTCCAAATCAAACAGTAG SEQUENCE_INCLUDED_REGION=34,223 P3_COMMENT=CL is CCAAATCAATCTTACCAGAACA P3_COMMENT=CR is GAAGCCCAAACAACATCATC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1044 SEQUENCE_TEMPLATE=NNTNCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTAGCCTGCTTCATGGCAAGGAAAACAAAGTTATCTCAGCCTCATTTAGTACATTTCCTGCCCCACTCCTNNNTAAGAATCAATTTCTCCAATGAGCCCTGGTTCCTTTTACTAGGAAATGGTATTTAGAAACCACAGTCTGGGCTTTAGGGGTAGGCATTGGCCTTTTTAGTAACAGAACTAGTATTTTGGTATTTNAGGTAAATAAAACATATTTCAAATTCAAATTTAAAA SEQUENCE_INCLUDED_REGION=35,234 P3_COMMENT=CL is CATGGCAAGGAAAACAAAGT P3_COMMENT=CR is GCCCAGACTGTGGTTTCTAA P3_COMMENT=CM is ATCTCAGCCTCATTTAGTACATTTC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1045 SEQUENCE_TEMPLATE=CTGTAGGAAAACCTATTTNNTNATTTCTCGAAATATTTCCTCCCACCCATTTTTNCTGTACTGTCTTCCGAGATNTCCTATTATAGGTTGCACATTCCTAAAATCCTGAAAATCTGAAATCCAAAATGCTCTAAAATCTGAAACTTTTTGAATGCCAACATGATGCCACTAGTGGAAAGCTGAAAGTTTCACACCTGACCTCATGTGATGAGTCGCAGTAGAAACACAGGCACGTAACACAAAGTTTATTCAGCATTTCCAGAGGGAAAAAGATCTTCCCAG SEQUENCE_INCLUDED_REGION=0,282 P3_COMMENT=CL is TATTTCCTCCCACCCATTTT P3_COMMENT=CR is TGGGAAGATCTTTTTCCCTC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1046 SEQUENCE_TEMPLATE=GGAGCCATTCTCCTCGGTCTCGCTGGCCTGCCATGGAGAACACAAGGGCATNNGGTGGCCAGGCCNATGCAGCCAGGCTCCAGGCATCCCCAGGATCTCAGCCCCTCCAAGGGTACCCAGAATATTGAGGCACAGAGAGAAGCGACTGGCCTGAATACATACCTGGCTCCGCATCCCTGTGCCTCTNAGGACCCTAGACTCCCCTGATTCAGCCTT SEQUENCE_INCLUDED_REGION=0,216 P3_COMMENT=CL is GCCATTCTCCTCGGTCTC P3_COMMENT=CR is AATCAGGGGAGTCTAGGGTC P3_COMMENT=CM is GCCTGCCATGGAGAACAC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1049 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAATCAACATGGGATGGCTCAGCTGAAACCAACATGGGGTGGCTTCAGGCCATCCCAGGCATTCAGAGGGTTCGTTTAATGACATTCACTGAGGCCCTGTCTATGTCAGGCCCTTGGTGTTGAAGACGCAATCATGAACAAAAATGAAAATACAATGTGATGGTCTCCTGAGTGTCTGAATGCGCCAGGTGGCTAAGTGCTGGGGCTCTGGGGTCAGGCTGCCTGGGTCACATCCTGGCTCCAAACTGCTTTGCTATGGCTTCGGGCAGTTACCTCATCTGTAAAATGGG SEQUENCE_INCLUDED_REGION=36,293 P3_COMMENT=CL is GCATTCAGAGGGTTCGTTTA P3_COMMENT=CR is AGCCATAGCAAAGCAGTTTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH105 SEQUENCE_TEMPLATE=AGCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCTATAAAGGAGAGTATTTTAAAAGTGCCTCCCAGTAGGAAGAACAGTCACAAGGCACTGTTATATCAATTCAGTGTGACACAAGCCCTGATTATTTAATAGTATAACAGCAGTGAATCAGAGTTCTTTCATCTGACTTTGCTGACATTTCCAGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT SEQUENCE_INCLUDED_REGION=35,155 P3_COMMENT=CL is GTAGGAAGAACAGTCACAAGGC P3_COMMENT=CR is TGGAAATGTCAGCAAAGTCA P3_COMMENT=CM is TGTTATATCAATTCAGTGTGACACA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1051 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGTGAACTCATATAATTTTCTTTCCAGCCTGTTACTATACATATATGCATGATGTCCATGCTCTCATCTAAAATCAGCCTCTGCTTGTGTACTAGATCAGGGTTGACAAAATGCAGCCTGTGGCCCAAATCCTGCATTTTGTTTTGTTTTTGTACAGCCCGTAAGCCAAGAATAGTTTTTACTTTTTTTTTTTTTTTTTTTTTTT SEQUENCE_INCLUDED_REGION=36,207 P3_COMMENT=CL is TATGCATGATGTCCATGCTC P3_COMMENT=CR is ACTATTCTTGGCTTACGGGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1055 SEQUENCE_TEMPLATE=TGCNTGCAGGTCGACTCTAGAGGATCCCCCTAACATTCCAGTGGAGGAACTAAAACTATTATCAAGTGACCAAATAAATGAGCAAGATTGTTTCAGAGAATGAAGTCTGTGGGGGAAAATAATAGGTTAGAGTGATCAGAGACAGAGAACTGGGTGGATAAAGGGGGCAGGAGAGACGTTGCTAGAAACCACTGTGTACGTTGAACATATTCTGACTCTGATCCTCCCAATGGAAGACCCTTATTCTTATTGCTTCTTCTTCTTTGTCTCCCCAGCCCCTGGCACAGAGTGAGCACTTAAAAACCACCTGTGTACATATGACTGACCTTTCCGTCACCTGCCTTCCCATTGGCCTCTTCCTGCCCCTTCATCCACCTTTGTGTTTGAATATTCATTCACATGGCTTGTTCTTC SEQUENCE_INCLUDED_REGION=29,384 P3_COMMENT=CL is AACATTCCAGTGGAGGAACT P3_COMMENT=CR is GAACAAGCCATGTGAATGAA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1056 SEQUENCE_TEMPLATE=NNTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGTGTGTATTGAGGGAAGGGGAGATGTGTGCACACNTTCCAGAAAAATGGGGAGGTAGTTTAGAAAGTNTTTCTCTGTAACAGCACAG SEQUENCE_INCLUDED_REGION=36,89 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1058 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCAATTTATAGGAAGTGAGGACAGGATAGCATGTTAAAGGGTATGGATGGCTTATAGGAGAGGGAAGGTTGAACTGAGTCCCCTGAAAGAGACCTGAGAAACATATCTACCAAATACAATGTTAACTTTGTTGGGATTCTGGTCCAAAGAAACGTACTAAAAAATATGGGGAGAAATAGAGGANTATGAATATGGCCTGAGTACTATAAGATATTAAAGAAAAATTATTAACTTGCTAGGTATGATAATGATATTTTGGTTATGTTTTTTAAAAAGTTGTTAAAAATACATAGTGAAGTACTTCAGGTGA SEQUENCE_INCLUDED_REGION=36,313 P3_COMMENT=CL is TAGGAAGTGAGGACAGGATAGC P3_COMMENT=CR is TCTTATAGTACTCAGGCCATATTCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1059 SEQUENCE_TEMPLATE=GGTGGATGCTNAGGTCGACTCTAGAGGATCCCCCTTATCTATTTACTTTTGCTACTTAAGTAAATAACTTATCACATCATAAGTGCATTGCNTTTGANGGTTTCAGTTGGTTGGCTTAGCACATGGTAAACACCAATTNGGCATTCTCTCTTGTNNAGTNGCCATTTGCAAAATGNATTATTTCAGATTTGCTGAAATACTGAATTTGTAAGTTTACACTCTGCTTATTCATATTTNTAAATAACTCTTTTGCCATTNGGTCCCAAACCCATGTGCTGATGTGTCTGCNGGGTCTCATTTCTTCCCCTGTATTAGTATCTGA SEQUENCE_INCLUDED_REGION=33,289 P3_COMMENT=CL is TTTCAGTTGGTTGGCTTAGC P3_COMMENT=CR is ACAGGGGAAGAAATGAGACC P3_COMMENT=CM is TATTTCAGATTTGCTGAAATACTGA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1064 SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGCTGTCAGTTTGCCACAGTTCTCACCATTCCCTAATCTATACCGCCCCTGCTTCATTCTTCATTCACTACTTGTGAGCAGACATCTGAGATGACTCTGGATTTATGTTTTAATCTCTTTATTTAAAACACACACACATACACTGCACACACACAAGTCACCACAATCACAACCACAAAGAAGTGCTTGAGTATGCTATAGCATGACCTTTTCTTCACATCTCTGCTCAAATATCTCCTTATCAGAGAGGCCTTCTCTTACTACCTTCTAAAATAGGACCCACTGCCCCTCTATGGGTCTCATTTCTCTTCACAGTACTCCAACACTTGACCCATTACTGATTCACTTGTCTACTCT SEQUENCE_INCLUDED_REGION=37,358 P3_COMMENT=CL is TTCCCTAATCTATACCGCCC P3_COMMENT=CR is CAGTAATGGGTCAAGTGTTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1065 SEQUENCE_TEMPLATE=ATGCCGAGATTTGTTTTAAAGTTGGAATAGGAAAATTCCTTCTCTAACATATTTTATACCCCCTGTGGATGACCCTATACCTTACTGTCTAAATNTTGACTTTCTGAGAGTNNGAGGNNGCACTGCTGTTAATGACAATTAGATTGGAGATAAAATCCAAGACTCTTACAGNCAAACTTGGACACATGTCACCCTACCTATGAAGTGGGAATTAATACTGACCTAAAGACGATGATAGTATCAAGAACGAGGTCACGGGTTTTAGAGCAACTAG SEQUENCE_INCLUDED_REGION=0,274 P3_COMMENT=CL is TTTATACCCCCTGTGGATGA P3_COMMENT=CR is CCTCGTTCTTGATACTATCATCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1066 SEQUENCE_TEMPLATE=NTNGCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTATTCAATTTCTTTACTGCCTTCAAAAATTAATTCAATCTGTTGAGCATGAATTACCTTATGATCCCCTTTTGTATTGTTCTATCAATGTGATCTGTATCTCATGATGTTCAATTTTTCCACTGCACACACAGNCCCAGTCTAAATAAATGCTGGCTGACTAACTTTCTGCTCACCTCTTGCTGCTCAGATCTTTCTTAAAGAAAGGTTTTGCCATTCAGTTCCAAGGAGCACTATTGGAG SEQUENCE_INCLUDED_REGION=35,242 P3_COMMENT=CL is TTCAATTTCTTTACTGCCTTCA P3_COMMENT=CR is GTGCTCCTTGGAACTGAATG P3_COMMENT=CM is AAATTAATTCAATCTGTTGAGCATG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH107 SEQUENCE_TEMPLATE=GCATTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTCCTTCTNACCCTGAACCAGGAAACTCACCGGCAGGGAATCAAGATGGGAAGTAAAAAGAAAATATTCCCCTAAGTCAGGNTCAGATTCTATCATGCCTGGCCACCTGGAGAAGATAGTTGGGTTTAGGGNNCAGAAGAGAAGGGNTTAGNTGNNCAGANCACANCCTNATCANTGTACATCCNCAG SEQUENCE_INCLUDED_REGION=36,188 P3_COMMENT=CL is ACCCTGAACCAGGAAACTCA P3_COMMENT=CR is ACTATCTTCTCCAGGTGGCC P3_COMMENT=CM is CGGCAGGGAATCAAGATG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1070 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGCTATATAGCCAAGTCGGTATTTATANNNTGTTTCCACCCTTGATAATTTCATGCCAGTACTCATCAGAATGGAACACTGACTTGGCTCTGTCTGTCCTTATTCTCATCTCCATCCCCTCCTTTCCAACACACTGCAATTCTAGATTGAAGACTGCACATTTATTTATTTATTTATTTTTTTGAGACGGA SEQUENCE_INCLUDED_REGION=36,193 P3_COMMENT=CL is CTTGCTATATAGCCAAGTCGG P3_COMMENT=CR is ATTGCAGTGTGTTGGAAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1075 SEQUENCE_TEMPLATE=TCTTCATGCTNCAGGTCGACTCTAGAGGATCCCCCTTGAATGAGGAGTNNCTTCTTATGGATTAGCAAAGCAAGTGGTTTCTTGAGATAGAATCTACTCCTGGTGAAGGCACTGTGNNNNTTGTTGAAATGNCAGTAAAAGATATAGAATATTCCATATTCTTAGATGATAAGGCAGTGGCAGGGTTTGAGAGGATTGACTCCAGTTGTGAAAGAAGTTCTACTGTGGGTAAAATGCTATCAAACAGNATCACAGGCTACAGAGAAATATTTTGTGAAAGGAAGAGTCAATCAATATGGCAAAGTTCATTGTTGTCTTATTTTAAGAAATTATCACAGCCATCTCTACCTTCAGCAACCAC SEQUENCE_INCLUDED_REGION=34,327 P3_COMMENT=CL is TGGATTAGCAAAGCAAGTGG P3_COMMENT=CR is TTGCTGAAGGTAGAGATGGC P3_COMMENT=CM is TTCTTGAGATAGAATCTACTCCTGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1076 SEQUENCE_TEMPLATE=AGGTGAACGTGTGGTTAAAACTAGGCAATTGGTTAAAAATCAATTTAAAAAACAGGCCTAGAAACAGTGACCACACCTCAAGCAATGATTATCCCTAGCACTCAGATTATGTTCTTGAAATACCATTTTCTGCTTTCAAAAGAAAGACATGAGGGCTTCTTGAAGAAATGGCTGATACCAAGCCTGCAGTGAAAAATGCACATGATGAGCCTGGAACATGTTGTCATATGAGTTAAAAAGGAAG SEQUENCE_INCLUDED_REGION=0,244 P3_COMMENT=CL is TGAACGTGTGGTTAAAACTAGG P3_COMMENT=CR is ACAACATGTTCCAGGCTCAT P3_COMMENT=CM is TTAAAAATCAATTTAAAAAACAGGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1080 SEQUENCE_TEMPLATE=CAAGGCACGTCTCAGGGTCCTGGGATCTGGGACAGGTAGGGACATTGCACCAGTCGAAGGGGAATTTTGAGGGTCTGGGGCCCTCCACTTCCGTCCCCCTCCAGCCTTACCCTCCACCTCTGGGTGCTTCATGAGCAGCAAGAAGCCATAGCGCAGGGTGGTGCTGACCGTCTCGGTGCCTGCAATGAAGAGGTTCAACGTGCTCATCATCAGGTTCTTCAAGTAGAACTCCGTNTTGGGGTTCTTCTCCTCCTGCAGGGAGAGGGGGCTTTAGG SEQUENCE_INCLUDED_REGION=0,275 P3_COMMENT=CL is GACAGGTAGGGACATTGCAC P3_COMMENT=CR is ACGGAGTTCTACTTGAAGAACC P3_COMMENT=CM is AGTCGAAGGGGAATTTTGAG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1082 SEQUENCE_TEMPLATE=AAACACTGTTATTCAATTTAATTTTGCTTGAAACCAATACACATGTATTTGAAATCATATTGACTGGGAATTTGGATCCTAGCAATATAAATTCCCCACATCACAAAGTACTTGAGGTGCTGAATGAACTAAAATAAAAGATAACTATTTATATTTATTTTNGTTCAATCATAATAATGTATTTAATTTATTATGTTGCAGCCCTAAAGAATTAAAGGGAG SEQUENCE_INCLUDED_REGION=0,221 P3_COMMENT=CL is TAATTTTGCTTGAAACCAATACA P3_COMMENT=CR is CCTTTAATTCTTTAGGGCTGC P3_COMMENT=CM is TGTATTTGAAATCATATTGACTGGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1083 SEQUENCE_TEMPLATE=TTGTTCAAGGAGACATATACAAAGATGTATATTTTTGAGTTGTTAATTGTTACAAGGCTAAGAAAATCTTATTGTCAAACAGCAGGGGGATGAAAAAATACACTGTTGTTTGTTGGTCCAGCAGAATGCCTAAAATGAAAATGAATGAATTGGATTTGAATGTATCAACATAGCAAGTCTCAGAGATATAATGTTGTGGCTAAAAAAAAAAAAAGTTGAATATAGNGTATCAANCATTTAGGAGTTTATC SEQUENCE_INCLUDED_REGION=0,250 P3_COMMENT=CL is GTTCAAGGAGACATATACAAAGATG P3_COMMENT=CR is TATTCAACTTTTTTTTTTTTTAGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1084 SEQUENCE_TEMPLATE=GTGTGAGAGAGATAGAAACAGAGAGAAGAAGAAAAGAGGCAGATTGGAAGTGTGAAAAAAATGAAAGAAGAAGAAAAAAAGAGTCTAAATATTCAGAAATGTAAGTGCTGCCCTCAACTGTTCTTTACCCACTTAATTCTGCAATTTTGAAAACTAGATTGAATTCCTTTGCAAAACCCTTGCATCATGGATACCCGAGTTAAACCGTTAATTAAAAGACATTAAACATGGCCTGGTGGCAATCTCCACGNCTCCAAGTCAGGAGCAGGAAAGGAAAAACAGCAG SEQUENCE_INCLUDED_REGION=0,285 P3_COMMENT=CL is AGAGGCAGATTGGAAGTGTG P3_COMMENT=CR is CACCAGGCCATGTTTAATGT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1091 SEQUENCE_TEMPLATE=GGGGATGGAGAACTGCCCTCCTGACTCGCCCTGCCCAGGATCTGAAGTTGAAAGAAACAGAAAATCTTTAAACATGATTCTTATTTTGGTTGTGTGTTGAATCTGTGCCTTCCCTCTGAAAAACTCAGGGTTGCCCCAGGTAGGATTTTCCCCAGAACACCAGGGAGAGCACAAGATCAGGTTCCCAGTGCCAGAATGATGGTCAGGGAGGCATGTAACTGGTCAGACAAGGGCATCTGCCAGTGTAAACAAGTTTCTCATGAACT SEQUENCE_INCLUDED_REGION=0,266 P3_COMMENT=CL is CCCAGGATCTGAAGTTGAAA P3_COMMENT=CR is ATGAGAAACTTGTTTACACTGGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1093 SEQUENCE_TEMPLATE=CAGGCTTCTCCATTTTCATGCCTACTTCTGCCTCCTGCTAAGTTGCATCAGTTTCAAACACAAAGGTTAAGATGTGTTACCTGGGTTAAGATGTCTTAGCCAGGGGTCAATCCATGACTATTACACTGTGGGGAAGTCTAGGTGATACTAGAGGGNTCAGGGTGCCTGGGTGACAGGTGATCACTTAGAGGGNTCCTGACAGTGCTTATTTACCANCTGGTACAGAAGTTTTTCAGTATCTTTAAACTGGTATGACTGTCCAGTGCATATTGGCTGACTATCAGCCTGA SEQUENCE_INCLUDED_REGION=0,289 P3_COMMENT=CL is TTTTCATGCCTACTTCTGCC P3_COMMENT=CR is CAGGCTGATAGTCAGCCAAT P3_COMMENT=CM is CCTGCTAAGTTGCATCAGTTT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1095 SEQUENCE_TEMPLATE=TNNTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTAAGAAATGGAGATTAATTTCTTTAGTAGGAAATGAGGAGCCTTTCATTCATTCAACACATATTTATTGAGTGCTTTCAGTGTGCCAACATCATTCTAAATGATTGGGATACATGAGTNGGAGTNAGTNAAGTGGACAATGGCAGCATTTTAGAAAAGGATTAAGGTAATCAACACTACTTTTTNAGAACAATGNGTCTGTGCCTTAAAAGTGTTTTGTGTGTGTGTGTGTGTTTAGGGGAGATGATCAGGACGATGGGGAGGCGCTATCAGTCAATGTATTGTTTGGGTGAGTGGTGATAAGTGCCTAATACACAAAGCGA SEQUENCE_INCLUDED_REGION=36,323 P3_COMMENT=CL is TTTCTTTAGTAGGAAATGAGGAGC P3_COMMENT=CR is TTTGTGTATTAGGCACTTATCACC P3_COMMENT=CM is CATTCAACACATATTTATTGAGTGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1096 SEQUENCE_TEMPLATE=ACTCCCAAATCCAGAGATTTGTGTGTGCAAATGTGTATGTNTNAACGTTCAAAATTGAAGACTGTCTAACTCAGCGACCCTCAATCTTGACTGAGCATCAGGGTCATCTAGAG SEQUENCE_INCLUDED_REGION=0,113 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1103 SEQUENCE_TEMPLATE=TCTGCATCCATCTATTTTTCAGATAAAACCATGGGAAGCATTTAACGTNCTAGTAAGTNTAAACAGTAAAATTTCCTTCCTCTGCTTTTCTCTTCTAATAACAAACAATAACTTAAACAGTTACACAAATACATATTCTTACAATAAAAATAGTTTATAATAACACTGTCCCAATAAAGACCACTATTAACTCTTACATCAAAAGGGAGTGGAGAAGAAAAATTTATATTAATAACTCCTTATCTCCCCAACTCTCCACCTCCCCTCAAAAAAAACCTCTGTGGAAAATTAATGTAATTAAG SEQUENCE_INCLUDED_REGION=0,302 P3_COMMENT=CL is TCTGCATCCATCTATTTTTCA P3_COMMENT=CR is TAATTACATTAATTTTCCACAGAGG P3_COMMENT=CM is ATAAAACCATGGGAAGCATTT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1109 SEQUENCE_TEMPLATE=NTTTTNNNATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGACCCTAGAGTGTCTTCTGAATAAACCATACCAAGAAGAAAAAATCTATGATGACACAGTCTTGGTTTCTCTACTGGGACATTGCTAAGATGTGGGTTTCCAATAAAAGGATATTGCTACCTTCAATATGAGGCCTAGTTAACTGACCAACCCCACTGAGTAAGCATCTTTTGACATTTCACTGATTTCAGGACTAGAAAAAATGGAAAGTTCACAGTCCCTAGAAATCTTCTTCTAGTCTCAGTGTAAATCTTATATATTTGATGGGATGTTTCAAACTTCTCCCATAAATGAA SEQUENCE_INCLUDED_REGION=38,300 P3_COMMENT=CL is GAGTGTCTTCTGAATAAACCATACC P3_COMMENT=CR is TTCTAGGGACTGTGAACTTTCC P3_COMMENT=CM is AAAATCTATGATGACACAGTCTTGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1113 SEQUENCE_TEMPLATE=TCACCTTTTCAGGCCCTGGCAGAAGGGTGAAGGCAAAGGCCCCAGACACAGGNNTAGAAGAGATGGNATCTGGNNGCCAATGCAAGACCCACCTTTTACAGATGGGAG SEQUENCE_INCLUDED_REGION=0,108 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1123 SEQUENCE_TEMPLATE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTCAAAACTATTAAGGGNATAAATAAAAAGTNGGGCAAGTNCATTTTTGGTTTAAAATGNGACTNTTAAAGCAGAAGGGAGTNTCCTTCCCCCTCCCCCACAAAGAATATTTTATAGATGTGAAAATACTAAGAGATTAAAGTTTCAGCAGAAATAAATATCTCAAAGGAGGCACCATACTATTTCCTTTCCATATGTTTAGTANNTCATTTTCACACATCGTTTGTAGTCTCCTAAGTTTAGACAAATCATCTTAG SEQUENCE_INCLUDED_REGION=34,256 P3_COMMENT=CL is CCTCCCCCACAAAGAATATT P3_COMMENT=CR is AGGAGACTACAAACGATGTGTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1139 SEQUENCE_TEMPLATE=TTGCNNNCCTCCAGGTCGACTCTAGAGGATCCCCCTTTGTTGTNAAAGCCTCAGTGTNNCTTCAGAGCACAGGGCTTATTTTTACTTTTATGTCCTGACTTNNNNTGGGCCGCCTGAGTCATTCAGAAAAGGTGGCCCAGGGACATGAACACCATTTCTTTTCCTTAATTCCCATAATTAATTGCTTTTAAATTAGGGGCAATGACTCTTTGGCAAATNACCCTGCCAAATACTTGCTTACTCCCCTTTGTGAG SEQUENCE_INCLUDED_REGION=34,220 P3_COMMENT=CL is TTCAGAGCACAGGGCTTATT P3_COMMENT=CR is TAAGCAAGTATTTGGCAGGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH114 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGATTTTTGTTTCAGAGGTTACCAGGAAAATAATTACACCAGTCTGACAATTTTAAATCAAGATTGAAGTATTGCTTTGGTTTCTTCTGAGATTTTGAGTNCCTAAATGAACATATTTGCAAAATTATTAAATATGAAAGTGGGTGGTAGTTTGGTTATTTTTAAAATCCTGAATAAATTTTTATATTTATATGGATTTTGAGGTGTCCCCATTTTATTTTTTCCTCTTTAACTCTGTCATGTTCATTTGCACCCCTTGTTCCCCTGACCTGTA SEQUENCE_INCLUDED_REGION=35,275 P3_COMMENT=CL is CTGATTTTTGTTTCAGAGGTTACC P3_COMMENT=CR is TACAGGTCAGGGGAACAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1146 SEQUENCE_TEMPLATE=CAGGTCGACTCTAGAGGATCCCCTTTGAGTNNACATGCTTATAAGAGATTACATTTATAATACTTATGTGATATTAGTAAATTAAGCCTATTATATAACCAAGCATTAATTAGATTAGAATGCTTTTCTTTAGAATTAAGGATCAGATTATACAGTTGGGCTACAAACTTAGATGTCTGTAGTTGCTGGGCAAGTCAAATCAGCATGTGAAAGGGAAAGATAAGACAGTNTGGGCTGTGGTGGTGTGATAAAACTGGAGAACATGGACCCCTTAGGCCAGCCGCCACACACCAGATTTTACTAAGACACACTGGTGGGGA SEQUENCE_INCLUDED_REGION=23,297 P3_COMMENT=CL is AGAATGCTTTTCTTTAGAATTAAGG P3_COMMENT=CR is CCCCACCAGTGTGTCTTAGT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1147 SEQUENCE_TEMPLATE=AGGTCGACTCTAGAGGATCCCCCTACTAGGGAGGCTGAGGTGGGAGAATCACTTAAGCCCAGGAGGCAGAGGTTGCAGTGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT SEQUENCE_INCLUDED_REGION=22,60 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1151 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAATTTGCCAGTNCTGGGTGGGGGCAAATCCTCGAGGGGTNCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=36,37 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1156 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTATTTACTTAATGGTAACCGTAGTAATAATGGCTATCTTTTAATGGTNNTGGGAGCGCTTAGTNTTTGACCACTTACTGAGTGCTAAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC SEQUENCE_INCLUDED_REGION=36,88 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1163 SEQUENCE_TEMPLATE=GCNTNCCTCCAGGTCGACTCTAGAGGATCCCCTGGTGTNNNCTGTCACTCTTCCCACTAGCCGGACTACACACAGACAGACTATACACTTCCAAGCCTCCTAATTTTGCCCATGTTGTTCTCTCTACCCAGAATACCTTTCCCTAAGCTGCCTGTAGGCCCTCTCAAAACATGCTAGTTCACTTTGTCAATGCCAGCAGAAGAATCTCTGTTGCTGCTATTAATGTCTTTCAAAGTCTCATGTGCTTTGGTCAGGCCTACCCAGGATAATCTCCATTTTNATTAACTCAAAGTCAACTGATTAGAGACCTTAATTATATCTGAA SEQUENCE_INCLUDED_REGION=32,292 P3_COMMENT=CL is TGTCACTCTTCCCACTAGCC P3_COMMENT=CR is GAGATTATCCTGGGTAGGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1166 SEQUENCE_TEMPLATE=TGCATCCCTCCAGGTCGACTCTAGAGGATCCCCTTAAAAGTNNACTGCTGACTGATAGGGTNGAAAAGAAAAAAGAATGCCTGGGAGAAGAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTNCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGC SEQUENCE_INCLUDED_REGION=33,60 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1177 SEQUENCE_TEMPLATE=GACTCTAGAGGATCCCCCTATATAATGGTNAAAATAATGTACAGTTAATGTGACTTGTTACAGGTTTTTACTCTAAGGTNATCACATAAATGAGTNNAGGATTCAAACAATGTTTGTTGTTACTGATGTTGCGAGACTGGCTTCAAGGTTATTTACAGAAGTGGTTCTAGGAAGAGTCATCTTTNGTCAGATGCTGACACCCACTGGTGAAAGATACAAACCTTTCCTAATAGAGGNTCTCTAGACATA SEQUENCE_INCLUDED_REGION=17,232 P3_COMMENT=CL is AGGATTCAAACAATGTTTGTTG P3_COMMENT=CR is GGAAAGGTTTGTATCTTTCACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1184 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCTGTTCACATGAGGNNATGAGGACACTATCTTTTCTAGTNAGAAGTGAAGATGTGACAGGTTTTGTTATTGGAATGAGGGAGAACTTCGTAAGATATTCCCTTTTTTCGCCCCTACAAACCCAGCATCTTTAAAACCCCCTGTCTGAAAGCGTGACTCGTCTGTTGCCTTTCCACTCTGGTCAGTGGGTCCTCGAAAACACCATAACATGTTTCAATAATTCTGACAAACCCATGAAGATTTTTTAAAGTTAATGAATCATTCTTTCTCCTGAAAACACATAAAAACACGTTT SEQUENCE_INCLUDED_REGION=35,292 P3_COMMENT=CL is GGAATGAGGGAGAACTTCGT P3_COMMENT=CR is CGTGTTTTTATGTGTTTTCAGG P3_COMMENT=CM is AGATATTCCCTTTTTTCGCC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1185 SEQUENCE_TEMPLATE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTAAGTNNAGAATGTTTTTTTTTCAGGAAACCTCTACAATGCAGATGCTAGAGATGAGGGGTACCGAGCTCGAATTCGTAATNATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG SEQUENCE_INCLUDED_REGION=33,57 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1186 SEQUENCE_TEMPLATE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTCACCTGGTACNNCNGNANTGANTGATCCGGATAAGGACAAAAAACGCCAGAAGAAAAGAGGCATTTTCCCCAAAGTAGCAACAAATATCATGAGAGCATGGCTCTTCCAGCATCTCACAGTAAGTCAAGATGAAAGAGNNAAAAAAAATAGTTTTTGTTGTTTTGCTAGCAAGTTATACGGTCTCATACTGATTTTTTTTTAANCTAAGGTGCCTATTTTCTTATTCTTGAAATCTGATGACTAACAGACTAGTTAAAGTTTTTTTTTTCATCAACTATTACCATCCAA SEQUENCE_INCLUDED_REGION=35,292 P3_COMMENT=CL is TGATCCGGATAAGGACAAAA P3_COMMENT=CR is TCAAGAATAAGAAAATAGGCACC P3_COMMENT=CM is ACGCCAGAAGAAAAGAGGCA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1187 SEQUENCE_TEMPLATE=TGCATCCCTCCAGGTCGACTCTAGAGGATCCCCCTAGAAAAGAAATAAGATACTGGGTCATAGGATTGCCCCTGCTTCCTGAAAGCATCCAATCCAGAGTAAAGTTCCATTTCTTTGAGGCTCCTCCCAAATACTCTATCACAAGTNCAAATCCTATAATAAGGCTTTTCTAATGCACTCTCACCGAGAAGCCTTATGGTCCCCATGGTGTGTGTTCTCCCTTGTTGCAAAGACTCAATAAACTGAAACTTGTTCAGTTCCAGGTATTCCTGGTTGTCTTGATTGGAGGGAATTGATAAGTCTATAGGGGTA SEQUENCE_INCLUDED_REGION=33,279 P3_COMMENT=CL is TACTGGGTCATAGGATTGCC P3_COMMENT=CR is TCAATTCCCTCCAATCAAGA P3_COMMENT=CM is CTGCTTCCTGAAAGCATCCA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1188 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTAGTNTTAAATGTTCTCATGAAATTATTCATTTTCTAAAACAAAGTTTTCCTTCCATGAACCAAACAGGAAAAATCCTTTTCATTCTTCGGTCTCTATGGCCCAAAATTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTG SEQUENCE_INCLUDED_REGION=36,114 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH119 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGGACAGATTGAGTNTAGAGAAGTGAAAATACCTTCTTCCTCCAGGCCAGGTTAGAGAAAATCAGGCAAAGGAAACAAATTTCAGTGCTGTGTGAAATGGTCTGACATGGACATGGATACCTGTATAGATAAANCCAAGATCTGAACAAAAGTTACCCATTTTGGAGACAGAATGTCCTCCCCCAGAAAGGTTCATGNTAATCCTACCAG SEQUENCE_INCLUDED_REGION=35,212 P3_COMMENT=CL is GGCAAAGGAAACAAATTTCA P3_COMMENT=CR is CATTCTGTCTCCAAAATGGG P3_COMMENT=CM is GTGTGAAATGGTCTGACATGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1190 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTATACATCTGTNTATCTTTCTATTTCTCTATCTATCTATAAACTCCAATATACAAATTGGGAGAAAAAGAGTTGTTCTTGTGTTGGTGTGGATAAAGAAGAAAATAGATAGAGAGAAGCCAGTGATAAAAGCAACATCAAAGTAAATTACATTAAATTTCAATAAAATATAANGACCCAAACACACCAGAAACTGCCAATCATATATGACGTCTGCTCTTATTCTCTGCTTTTNGTCTTGTACATTTTCTAAGCAATGGACAATTTCTTCGTCAACAAAATTCCATCTTCTCTCAAATATGCAAG SEQUENCE_INCLUDED_REGION=36,306 P3_COMMENT=CL is TTGTTCTTGTGTTGGTGTGG P3_COMMENT=CR is CGAAGAAATTGTCCATTGCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1192 SEQUENCE_TEMPLATE=GCTTGCATNNCTNCAGGTCGACTCTAGAGGATCCCCTGCAGGACTCTTTGAAGAGTAAATTGAACAATGATGTCAGGCACCAAGAAAGACGCCTGGAAGAGACCAGGGCTCCATGAGAGTNAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGA SEQUENCE_INCLUDED_REGION=36,87 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1193 SEQUENCE_TEMPLATE=CCTTTTGTNTCCTCATTTGTNAAGTGGGAAANATGGAATCTATCTAAGGCTTGGNAGGTCACATAAAGAAAGGCACTAGAATGATTTCNTTCTNTCTCCTCACCACCCGCTCGACCCTCCCTCTNACTGCCTTNAGGGTTGGCGAAAGANAGGAGACAGGCGAGGTTANGAGATTTNATAGGGCAGGTTTCACAAGGTCAGNGAAATTACATTAANTTAACACTGNCTACATTAAACATGGAATGCCTTTTTGGNGTGTTGGTCCCCACACTT SEQUENCE_INCLUDED_REGION=0,273 P3_COMMENT=CL is ATGGAATCTATCTAAGGCTTGG P3_COMMENT=CR is CAAAAAGGCATTCCATGTTT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1195 SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTGGGAGCACATGACAGATAACACACTGTCCCTGTTTGTCCCCATCCCTCAGCAGGCATGTTCAAGTTAGGGGCTGGGGGTGAGGAGCAACTNACACNNNTGATCCCAATGGAAGGACCACTGCACTCAGTGCTACAGGAGAGACATAGCAAGATAGGAAAGTGGGAGTGTGTGACTGCTTCTGGCTGGGGGATCAAAAATGGTTTAATGTAGATCTAATTTTTCCTGAGCACCACTTCTATTATCTCATTTAATCTTTATAACAACCATATGGGATAGATATTATCCCCATTTTACAGATGAGAAAACTGGGGCTCCAGAGTAGGGGT SEQUENCE_INCLUDED_REGION=37,331 P3_COMMENT=CL is TGGGAGCACATGACAGATAA P3_COMMENT=CR is GAGCCCCAGTTTTCTCATCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1199 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTCTATTTCCCCAATTTCCTATAATTCAATAAAAAGTCAGCAATAACATGTCCAAGGGCATGTTATCTCAAATGTGGATTTTAATGAAACACAAATATGCCCATGCATGTTTTAGCAAATTGTTCTGGACCATGCAAAAATGCATTAAAAGTGGAGGCATATTTACATTTGTTTTGCATTTGTTATTTAAGAGGTCAGACTATGCAAAGTGCATCCACTTTTGTCAATAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA SEQUENCE_INCLUDED_REGION=36,231 P3_COMMENT=CL is CCTATAATTCAATAAAAAGTCAGCA P3_COMMENT=CR is AAAGTGGATGCACTTTGCAT P3_COMMENT=CM is TAACATGTCCAAGGGCATGT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1200 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAATGTTAACATGCTGTGACCAGTAAACCAAAGATGCAGTGATTGCTAGTAGGCTCCATCGTCGGCCCCTTGCAGGTGGTGGGGACATATTCAGGGGTGGTGGATATCAGTGAGCACAGCCCCTGACTGGTGGCTGCCCATGCAGAGTGTGGGGGTGGGGAGTAGATGGGGGGCTTTTCCTAATGCATCTAGCACCCAGTGTCTCTGGCTTCCTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC SEQUENCE_INCLUDED_REGION=36,218 P3_COMMENT=CL is TGCTGTGACCAGTAAACCAA P3_COMMENT=CR is TAGGAAGCCAGAGACACTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1203 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGTCCACATCCACTGCTTACTTACAAAAGAAAGTACAAACTTAGAGAAGGCCTTCCATGGTTTGGTTGCTACTGACTTTGTTAGCCTTACTGCCCACTATGCATTGGAACATTCCCATATTCCAACTAAGCAGGAGTGTTCACAATAAACAACATAGGCTCTTTATTCTCCTTCTTTCATTAATTTTCTTTCACGTTATTCCCTCACCCTGAACGCCCTTCTTCCTTCGTAGTGACATTTTAAAATCCACTTTACACATTCGGACCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTC SEQUENCE_INCLUDED_REGION=36,268 P3_COMMENT=CL is TCCATGGTTTGGTTGCTACT P3_COMMENT=CR is GGTCCGAATGTGTAAAGTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1207 SEQUENCE_TEMPLATE=TNGCATTGCCTNCAGGTCGACTCTAGAGGATCCCGATGTGCTGCAAGGCGATTAAGTTGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTNCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG SEQUENCE_INCLUDED_REGION=34,24 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1208 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTGCCTTTGAGATTTGTNCAAGTTATTGCTCATTATCTAGTTTGTNTCTTTTTATTGCCCTTGTCTTCTGTTTCATGGGTGTGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTC SEQUENCE_INCLUDED_REGION=35,81 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1214 SEQUENCE_TEMPLATE=NTGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCTTCTTTGAATGTGCATGTGTGTGTGTGTGTCCTGGATGGGATCCTACTGCTATCTCTTCTTTTTCTCACCCAGTGACATGCCTTTCTATGCAGATCCATCTGGATCTNNTNTAGTTTTGCTCACTGCTGCGTAGCTTTCATGGTATGAATGCCAGAAGCCATTTAGCACCTCCCATAATGAGGGGATTTATGTTTTTNCCAGACCAGTGGTCTACAGATTGAGGGGTACNGAGNCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGA SEQUENCE_INCLUDED_REGION=38,226 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1215 SEQUENCE_TEMPLATE=TTGCANNGCCTNCAGGTCGACTCTAGAGGATCCCCCTAGGCAGAGNNNTATCTCATTTTCTTTCATGGTTTAGGTNAAGTNACGTTAACTGCAGGCAGANGGCTAGTTTATAGTTTGAACAGACTCAAAATTNNTTTANACAGGACAAAGAAACAACAGAAGTACATATTATAAANCTTTTAAACTNTTCCCTAATATAACACTTCNTTTCCACTTGATACTGAAAATTTTTAAANTTCTCAACCTTAATTCAATTTACATCTATTTAAAAACATTATATAANACATTCTATATTANCAATGAATTGATTTGACTGTTTTC SEQUENCE_INCLUDED_REGION=35,286 P3_COMMENT=CL is CTCATTTTCTTTCATGGTTTAGG P3_COMMENT=CR is AAAACAGTCAAATCAATTCATTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1216 SEQUENCE_TEMPLATE=GCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCAGGGTNNTGACCTGTGGAGTCTCAACCCTAACAGCACATTAGCACCACTTGGGGAGCTTCTGAAAAATACTGGTACCCGGGGCCTTAGCATAGGTATTGTTCAAAACCTCTCAAGGTGATTTAAATGAGTAACAAGGGTTGAAAAACCNTGATTTTGGCAGCACAAACCAAAAGAGCAGGCAGGGCCGNGAGAGGGAAGTCAGTGGTACCAGAAGGTAGATGGGCTCCCTTGCAGGCTCCTTGTTCTCCTGCCATCACCAGTAGAACCTTCTGGCTGACAGACCAGGGACAAGTAGACTGGGTTCAAAGTG SEQUENCE_INCLUDED_REGION=32,314 P3_COMMENT=CL is TGACCTGTGGAGTCTCAACC P3_COMMENT=CR is TTGAACCCAGTCTACTTGTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1217 SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGGAAGACCAAAAAGACAGACCTCCCTTTCTTACTGAGAAGAGCGTAGTCAGACATGTCAAGTCTGGCACGCTGCTGCAGAAGGCGTGATTCATCTTAAGATCAAAAATTATTTCATCTTGCTTTCTTNNTCATAAGGGTACCGNGCTCGAATTCGTAATNATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATNCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGC SEQUENCE_INCLUDED_REGION=37,137 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1218 SEQUENCE_TEMPLATE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGGACTACAGGGATCTCTTAAGCNCGGGAGGNGAGGNNAACGTGGGCTGTGATCGCACACTTCCACTCCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTNGGGAAACCTGTCGTGCCAGCTGCATTAATG SEQUENCE_INCLUDED_REGION=33,71 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1221 SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTAAATGTNNCCTCTTCAGAAAAGCCCTCTCTGACCATCTGCTCAGAAGCAGTCCCATCCAGTGACTCTCCTTTGCTGTTATCACCACTTGAAGTTTGCAATTAGTGCATTTCTATGTGTACCTCCTGCTTTCCCCATTAGAATGTGTCTTAGTTCATGTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGNNNGCTAACTCACATTAATTGCGTTGCGCTCACTGC SEQUENCE_INCLUDED_REGION=37,164 P3_COMMENT=CL is CCTCTTCAGAAAAGCCCTCT P3_COMMENT=CR is ATTCTAATGGGGAAAGCAGG P3_COMMENT=CM is TGACCATCTGCTCAGAAGCA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1225 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAATAGAAAGCATTGTCTATCTGTAACCTAACCTAGTNCCTCTAACACTTCCTTGCACTTGTTGTTTACAAGGCTGCTTCCCCATTAGACTACGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATNGTTATCCGCTCACANTTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTNCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC SEQUENCE_INCLUDED_REGION=36,97 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1227 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTGATAATTCACATACCATAAAATCCAACCATGGAAGGCACACAGTTCAGCAATTTTNAGTCTAGTCACAGAGTNGTGCGATCACCACTACAGTCTAATTTCAGATGTTTTCATTACCCCTAAAAGAAATCTTGTACCCATTAGCAATTATTCCTCATTCCTGCCCTCACCCCCAGGCCCTACTCTTTATCGCTATAGATTTGCCCACTTGACATATCATACACATGGAGCCATACATATGTGTGCCCTTCATGATTGGCTTCTTTCACTGAGAATAATGTTTTCAAGGT SEQUENCE_INCLUDED_REGION=36,291 P3_COMMENT=CL is CCATAAAATCCAACCATGGA P3_COMMENT=CR is TCATGAAGGGCACACATATG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1228 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTTGCATGAACAAAATTGGAAAGGAATGTATTCTGTTTNTCTGAATTTCCTTAATTTGCTTCTTTCCTTAATGTNCAAATTTGAGATGTGTGATATCAACTTGGGAGACATATATCAACGATAATATTTTATAAGAGCATTTTCTAAAATAATACAAAAATGCAGAATAATAGGATGGATTTGTTGGAGAAATAATGGGCTTTGAATCTGGATATTTCTGGGTTCAGATCTCAGCCCTGCCACTTACTCATTACATGACCTTGCATGTGTCACTTCCCCTCTCAAAGTC SEQUENCE_INCLUDED_REGION=36,288 P3_COMMENT=CL is TGAGATGTGTGATATCAACTTGG P3_COMMENT=CR is GAGAGGGGAAGTGACACATG P3_COMMENT=CM is ACAAAAATGCAGAATAATAGGATGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1230 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCCCTCCACCTCTGGCCCACACACCAAATCACACTCTCTACATTTTCTGATTTCTGTATTGAGACCAAGTTTTGATTTATATTTCTGAGGGGTCAATCTGCAATTGACCNACGTGCCAAATCTGTAGACTATATCCAGTTTTCAAACTTATTTCCACATCCTTCAAAACAACTGCTCACACCATTGCTCATCTCCCTACTTCTGCATTTCAACACTACTCAGGGAGACGTGGGACAGNANGNTCTGTGGCACAGCACAGGCCCACAGTGGGCTCATCTAGTGCACTGTGTCTGGACCCTGCTCTGC SEQUENCE_INCLUDED_REGION=36,307 P3_COMMENT=CL is CCACACACCAAATCACACTC P3_COMMENT=CR is ACACAGTGCACTAGATGAGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1231 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTTTCCCCTCCTACGTNAGAGTNNATACCCAAGGAAGCGGAAGTAGAAGATGACTCTTCAAGAGANNTATCCCCTGCTGTTACATGTATACATACANGATACATACATATGGAATGCATGGGAAAGGAAGGCATTAGAATTCCACCTGAACTTTCTGTGCTTAAAAGATTAGAGGAGACAGTGTACAAAAAGAGAGATCATTGATTCTCCGCCCACTGCCTTGACTCTATGTGTGTTTTCTATTTTGATACTGTTGCTCCTCTTATTCACATGCTGTTTAAAATATACAGGGGTACCGAGNCTCGAATTCGTAATCATGGTCATAGCTGT SEQUENCE_INCLUDED_REGION=36,291 P3_COMMENT=CL is CAAGGAAGCGGAAGTAGAAG P3_COMMENT=CR is CATAGAGTCAAGGCAGTGGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1232 SEQUENCE_TEMPLATE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTACCCACCCCCTTCACCCCACCTGAATGAGATGATTTCTGAAGTCCTCAGCAGCCCTGATTCTAAGCCTCATAAGGAAGAGTAGGTGTTAATGGCATCCTAGGGCAATGGTAGGTGCCTGATGCAGATCTGCTGTGAGCCATGTGCTGGCATCACAGGGGTGGTTTATTAATTTCATTTATCATCTGGACAGCCCCTTCTTATAACGTACATCCTTGCCTCTTCTGAGGCGCTAAGATCCCCAAGGTGGCTCCTGTATCCAGAAACCCNTNGGCCTTCCCTCCCAGTTA SEQUENCE_INCLUDED_REGION=34,289 P3_COMMENT=CL is GATGATTTCTGAAGTCCTCAGC P3_COMMENT=CR is TTTCTGGATACAGGAGCCAC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1233 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAATATTATAGAACATTATATTTGCATGTATCCAAGTNGCATTATTGAAAAACTCATTTGGATNAACATTGAGATTTTAGGAGTNNCTTTAAAAGAGTNNTGTACATAACTACATTTAAGAAACTCCCTCATGACAATTCCATGAGATAGTTACTATTATCCACATTTTGCAGATGAAGATACTGAGACTTAACAAAAATAAGTAAAATTGTCTACGGTTATGAGTNTAGCAAGATACCAGTGAAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTC SEQUENCE_INCLUDED_REGION=36,247 P3_COMMENT=CL is GAACATTATATTTGCATGTATCCA P3_COMMENT=CR is CCGTAGACAATTTTACTTATTTTTG P3_COMMENT=CM is GCATTATTGAAAAACTCATTTGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1234 SEQUENCE_TEMPLATE=CTTGCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTTTGTNGTNNCTCTTCTTTGTNGATCAGAAATTACAGCAGGAACTACTGCTACTGGGTNAGGAAACCTAAATGCAATGNGGATCACTGGATNCAGGGTNNCACTTTAGTCGCCAAAGACAAGGTGGNATGGNTACTATAATAGACATCAGAATCAAAGCAATAATCAGAATAGCCTAAGAGATGTATGGCATTGGCTAGTTGATCATAATGTCTCCACAAGTGAAGTAGNTNGGCAGTCAACAAAATTCTTACTTGATTTGTNTANGGGNAACAACTCCATATCAAGTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTG SEQUENCE_INCLUDED_REGION=35,291 P3_COMMENT=CL is GAAATTACAGCAGGAACTACTGC P3_COMMENT=CR is AAGTAAGAATTTTGTTGACTGCC P3_COMMENT=CM is CACTTTAGTCGCCAAAGACAA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1236 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTACATGTNNNACAGGTCTTGTTTCAAAAACTTAATCCCCATGAAAGTTTCTGNTTGTACCCCAGATGGTAGTGTGTAACTTGCTTCTCCATCAGGGAAAGTCCTGCTGTCCATGTGAGGCTCTGGCTCTNCCATTTTCCACCCCTGGGCTGTGATCACAGCAGAGCAGGCTTCTAGAATNCATGTCCCCAGCACCCCATCCTTGCAGCAATGGCGAGGAGACAGCAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAC SEQUENCE_INCLUDED_REGION=36,230 P3_COMMENT=CL is TTGTTTCAAAAACTTAATCCCC P3_COMMENT=CR is GTCTCCTCGCCATTGCTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1237 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCTTTTCTTTCAGTNCTTCATTTCCANNATCGACTTGTGAGAAAAATGTCTTTGACACAGAAAGAAGTGCTTCTCTGGGCCACTGTNNNTCAAGGAAAAATCTAGAAAATCTGTTGTNTNATTTCAATAACATACAGTTTAATACCAAGAAAAACATATTTCTCGGGAACTCCTCCCTGCCCAGTNCCCTCTTCACCTAGTTAACTCCTACTCATGTCTATGGTGCTTAATGAACTGAAGGGCTCTGTNCATTTGAGGGNTGCCTGGGTNTGCATTTAGTGACCAGTATGTCTCCACAGTATCATGCACACGGA SEQUENCE_INCLUDED_REGION=36,312 P3_COMMENT=CL is GTGAGAAAAATGTCTTTGACACA P3_COMMENT=CR is GCCCTTCAGTTCATTAAGCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1238 SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGTTTGTNGTNTTGACTGTTTGTTTGTTTTTTTACTGCCTACAAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTT SEQUENCE_INCLUDED_REGION=35,44 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1239 SEQUENCE_TEMPLATE=TTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTACAACTGTGAGCAGCAATGCCGTCTTTCAGTATAGCATGCTTCATATTTGCAGAGATCCGCATGCACATAATATTCTCACCATCACAACACAGTTAAGTATGCTTGCACACGTGTGTACACACACACACACACACACACACACACTGAGAAGGCAGTCAGTGACTACCTTCCATTTTCCAACCACAAACTGCTGTCAGAATGAATACTACGTTTTAGCTTTTCAAATAACTGGAAATAACATCAGATTAAGTAAAGAAGACAGGGTAC SEQUENCE_INCLUDED_REGION=34,271 P3_COMMENT=CL is GCAATGCCGTCTTTCAGTAT P3_COMMENT=CR is AAACGTAGTATTCATTCTGACAGC P3_COMMENT=CM is GCATGCTTCATATTTGCAGA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH124 SEQUENCE_TEMPLATE=AGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCGAGGCCAGGAGGGTAGGTTGATTTCAAAGCGGGCAGATGAATGTCTTATTTAGGAGTAGGATTGGGTTCACTCAAATATAAGTTTTATTTGAATTGCAAAAGGCGGCAGGTTAGATGCTTGTATACATTCTCATGTATTTNTNATTTTAAACTCTGCTGTGCCTTGGTTACATCTTTGACCTTCTTAAGAATGAGTGAAGACATTTGTTTCATTTAGCATTGGTTGAGCCTCAGATGGAGGTTGGATATTTGATTATGAAAGATGGAACGAAATGTTAG SEQUENCE_INCLUDED_REGION=36,282 P3_COMMENT=CL is CCAGGAGGGTAGGTTGATTT P3_COMMENT=CR is GAGGCTCAACCAATGCTAAA P3_COMMENT=CM is AAAGCGGGCAGATGAATGTC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1240 SEQUENCE_TEMPLATE=GNTNGCANNCCTCCAGGTCGACTCTAGAGGATCCCTGGCCTTGCAGACATNACCAGACGACACCCAAGAAAAATGGCAGCGCAGAGCCGTGAGCATCACAGGAGGTCTCAGGGNCACGCCAAGGCCGCCTCCCAGGANAAAGCAGAACACCGGAAAAGACCTTGTGAAGACTTNTTATCTTCCCCTCCAAATGGTGTCTCTTGCTTTGGCTCTTGTCGAGTTGGGGCTTGTTGGAAGACCAGGTGAAGGCCCAGGGTNNCGAGCTCGANTTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAANTTGTTATCCGNTCACAATTCCACACAACATACGNGCCGGAAGCATAAAGTGTAAAG SEQUENCE_INCLUDED_REGION=35,218 P3_COMMENT=CL is ACCAGACGACACCCAAGAAA P3_COMMENT=CR is GGCCTTCACCTGGTCTTCC P3_COMMENT=CM is GCAGAGCCGTGAGCATCA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1241 SEQUENCE_TEMPLATE=CTTGCATNCCTCCAGGTCGACTCTAGAGGATCCCCCTGCAGAAAGTGAGAGAAGGAGGGGACAGGACAGGATGTCTGGGCCATCCCGATGGTCCACTTGGTCTGTTCATTTGCTGAGCGGCCTTGTTCCGTGAGTNCCCTGTGGNTNTCCAGGACAACAGATGCACCATCTCTAGTGCCATTTGAAACTAAATGAANGAATGAGGAGGAAAAAAAAAAGGAAACTGGTATCTGCTGCCCAACTGATANNTGCGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGT SEQUENCE_INCLUDED_REGION=35,220 P3_COMMENT=CL is GAAAGTGAGAGAAGGAGGGG P3_COMMENT=CR is ATCAGTTGGGCAGCAGATAC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1242 SEQUENCE_TEMPLATE=TCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTTTACTAGCCTGAGTNTTTTGTATATGAAATGAGAACAATGACCAATAGTGAGAAGACTTTTTTGTAGGACTGAGTNNAATAATACATGCAAAGACCCCAGCCCCTCAGTGCTCTCAGGTCAACACATATTAATTTCCTTCGTCTCTCTTTGAAGGTCAGAAAATTTGGACAGTGGCGTAGAATTACAGTGCTGTGGGCCAGTGTGCCATNTGATGGCAGAAAGCACTNNTTGGATCTCTCTGCATGT SEQUENCE_INCLUDED_REGION=36,250 P3_COMMENT=CL is TGTATATGAAATGAGAACAATGACC P3_COMMENT=CR is TACGCCACTGTCCAAATTTT P3_COMMENT=CM is GTGAGAAGACTTTTTTGTAGGACTG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1244 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGGCATTTGGCTGCTCTCCAGTCATTTAATCTGGNATTTAATNACAAATAATAGTTNTATTGAGTNNCACAAAGTCAATNCTTCTTACACTCTTTTATTCAGGCAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC SEQUENCE_INCLUDED_REGION=36,109 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1245 SEQUENCE_TEMPLATE=TGCNTGCCTCCAGGTCGACTCTAGAGGATCCCCTGGCATAATACATAAGATTTAGCACTTTGAATATAATGTTTTTTCATTGAGAACATTATTTTTCTTANNAANACAACCAATATTTTTGTAATTTATAATTAATATATGACAATCCCTGTAATTCTGGTATCAGAAGATCTGGTAAAATCTAATATGTTCCGTGTGTTGTAAGATTTTNNCTTTCTGAATATAAATAANGTCATAGTATCATTGAATGCTTTTATTCATTCAAAATCAGTTATTGAAGCAATCTCTGGGTAGCTATCCAAATATCCTCTTGCAG SEQUENCE_INCLUDED_REGION=33,283 P3_COMMENT=CL is TGGCATAATACATAAGATTTAGCA P3_COMMENT=CR is GCTACCCAGAGATTGCTTCA P3_COMMENT=CM is TATATGACAATCCCTGTAATTCTGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1246 SEQUENCE_TEMPLATE=NNNTGAGNTCGCNTGCCTNCAGGTCGACTCTAGAGGATCCCTCCACTGCTGTNNTTCACACAGTNGGGNATGCTGCAACCTTAGGGCCTTTTCACTCTTCTCTCTTCATAGAACACTCCTCCCTGAGACAGCCATGTAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCG SEQUENCE_INCLUDED_REGION=41,97 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1247 SEQUENCE_TEMPLATE=NNTCCCTCCAGGTCGACTCTAGAGGNNCCCCTTACATCTCCATAAGCAGCATGTGTNTTAATTGCTGAGCCCCAGTNACCACCAGACAATCTAATCAATGTCTCCATAATTAACCTCACCAAATGGAAAGTCATAAATTTAGAACTAAAATGAAATTCTGCTCAGTCTGCATATAGAAACTGACATAGCCCAAAAGGAGACAACAGGGTNNTTACCAGAATAATTTAAGAAAACCTTGTTTGGAAGGGAAGGAAAACAAAATAATGTGATACCAAAATGACCAGATGGATCATATACATTAATCGTAGTTGCTGGGTGNCTGCTGGAAAAATATGTCTCTCACA SEQUENCE_INCLUDED_REGION=31,313 P3_COMMENT=CL is TCTCCATAAGCAGCATGTGT P3_COMMENT=CR is GTGAGAGACATATTTTTCCAGC P3_COMMENT=CM is ACCACCAGACAATCTAATCAATG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1248 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCTTCTCTTTCTCAGCCTGTTTTCCTCATCTGTAAAATAAAAGGGTTGTATCAGACAATTTCTATTATCTTTCTCTAACTCATTTTGTCTTATATAGGTATAAATGAAAATACAAGAAATTGTCTTTAATCTGGACAAGTTCTATAGGACACACCGTGCTGAATCTTACAGAGAAGNACTTGGGTTAGAAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGA SEQUENCE_INCLUDED_REGION=36,191 P3_COMMENT=CL is CAGCCTGTTTTCCTCATCTG P3_COMMENT=CR is TCAGCACGGTGTGTCCTATA P3_COMMENT=CM is AAAATAAAAGGGTTGTATCAGACAA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1250 SEQUENCE_TEMPLATE=CTCTTCACTCCAGGTCGACTCTAGAGGATCCCTCTGTTATNTATAATTTNATTTGGAAAACTTAAACCTAGACAGGGATCATAACTTGGAAATCATCAAGTTGAAAAAAGAATCATAGATGGCCTCTGGAATTTNGTTCAAAAGAANACTGGAAACTGAAAATTNNGGGACTCATTTAAGTCATGTATTCAGTTTTTTAAAATATCTTTAAGANCNNTANNNNNNCCAGAGAAAAGTTGTCTATCTGTATGTGTGTGNGNGTGTGTGTTTGTGTGTGCACAC SEQUENCE_INCLUDED_REGION=32,250 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1251 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTAAAGGAGTNNGCCACATGGATGATTGGAGAAAGTGCATCCTAGGCAGAGGANACAGCAAGTGCAAATGCCTTAAGATAGCGTAAGTCTAGGGAGTGTGAGATTTGCAAGGATGCAGGGTAATTGAAGAAAAGTGAGTAAAGGAGCCAGAAAGAGATGGATGTAGAGAGGTATCCAGTGGCCAGACCATACATAGCCCTGTGAGCCTTTTTAAGGAGGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACA SEQUENCE_INCLUDED_REGION=36,218 P3_COMMENT=CL is CCACATGGATGATTGGAGAA P3_COMMENT=CR is TAAAAAGGCTCACAGGGCTA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1254 SEQUENCE_TEMPLATE=CTTTCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGGCCTACCTCTCACTCCCTCTAGCCAGCNTACCTCTGGCCAGTNTCCTGGCAGGCCATGCATTTCTAGCCCTCTCCACCTGTGCATGTCCTGTCTCCTCCCCGTTCTTCTCTGCCAAACTAATTTCTGTCCTCCTCATGTGGAGCCTGGACCCCCACCTTGAGGCCAGTGCCCATTTTCTGTGGCCCCATTGCACCTTCTAGCAGAACCCTTAAACTGCACACAGCAAGGGGTACNGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTC SEQUENCE_INCLUDED_REGION=35,231 P3_COMMENT=CL is TACCTCTCACTCCCTCTAGCC P3_COMMENT=CR is GGTTCTGCTAGAAGGTGCAA P3_COMMENT=CM is GGCAGGCCATGCATTTCTAG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1258 SEQUENCE_TEMPLATE=CTTGNATGCCTCCAGGTCGACTCTAGAGGATCCCCTGTAATATTGGTAGGAACAAATTCTTGTGCTAGATCCTATGTNGAGTNTAGACNGGCACTCATGGAAAAAGCCTGTGAGTTCCTCCCAGCAATGACTACTGGGACTTTGTACTAAACCATATCCTTTCAAGGGGCAATTATCAGCTTTCTATTGGNTATTAATTGAAACTACTGCAAAGACTAAAGGACATCAAATAATCTTGAAACTTGAAATACCCATGTTGTCATGGTGATGTAAGAGAAACATTTTAATGGGGATAGCAGTGCCCAGAAAAGTTTCATAATAAAAATGGGAAGTGGTTTATAGGGAATCATGCTGTGTGGG SEQUENCE_INCLUDED_REGION=35,325 P3_COMMENT=CL is GAACAAATTCTTGTGCTAGATCC P3_COMMENT=CR is ACAGCATGATTCCCTATAAACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1259 SEQUENCE_TEMPLATE=CTNGCANCCCTCCAGGTCGACTCTAGAGGATCCCCCTGTAAATATAACATGCTCACTAGAAATATTTTGTTTAAGGGGAAAACAAATATTGCATACCTTTTTGGGTTGTTCATTCAAAAAACCAAAGTAGTGTCTGTTTAGGGAGGGATAAGTNGATTTNCTATTAAAGGGNCTTTTCAACAAAAACAGTAGAAAGGAGACCTTTCATTTGAACAAGTCTTTATAACAAACAACACTTTGTTCTCTTAGTNNGAAAGACTATTTNCTTCTCAGGNACCAATACCTAACANCTAAAANCAGAAGATAAAGATGTATTGTTCGGAGCA SEQUENCE_INCLUDED_REGION=35,291 P3_COMMENT=CL is TGCATACCTTTTTGGGTTGT P3_COMMENT=CR is CGAACAATACATCTTTATCTTCTG P3_COMMENT=CM is CATTCAAAAAACCAAAGTAGTGTC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1262 SEQUENCE_TEMPLATE=CTTGCATGACTCCAGGTCGACTCTAGAGGATCCCCCTGTNCTGGGCAACCTGAAAAGCATTTTCCCAAGAGAGTNGTAAGACTGACTTCTTTCTTTGTNAGGTCTCTGCTTAAATGACATTTACTCACTGAGGCCTTCCTTGATTTCCTGACATTAAAAACTCACCTACAGCCCCCTCTGCTACCTATCCTAGTTTATCTCCCTTTTTCCAGAGTATATATCACCATCTGATAATACATTTTGACTTTCTTCTTTGTTATGTCTGTTTCTGCCAACTGGAAGGCAGAAGCTCTTTTTGACCAGAGTTGTATTCTCAGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTC SEQUENCE_INCLUDED_REGION=35,281 P3_COMMENT=CL is AACCTGAAAAGCATTTTCCC P3_COMMENT=CR is AAAAAGAGCTTCTGCCTTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1267 SEQUENCE_TEMPLATE=NTNCATNCTCCAGGTCGACTCTAGAGGATCCCCCTGGGAGCAGGTGTGTNAGGCCCCCACACACTGTAAAGCGATCCCCTTCCAAGGNGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGNTCACAATTCCACACAACATACGNGCCGGAAGCATAAAGTGTAAAGCCTGGGNNNCCTAATGNGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGANTCGGCCAACGCGCGGGGAGAGGCGNTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA SEQUENCE_INCLUDED_REGION=33,52 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1270 SEQUENCE_TEMPLATE=CTTGCATGCNTNCAGGTCGACTCTAGAGGATCCCTCCTNGACCTNGGGCTCCGGGACTGTGTACTCGGCGCCCCAGTCGAAGCGGCGCACCGGGGCNTGTACTTGTAAAACGCGAACTGCAGCGGCGTGTCGNGCTTCTGCGNGTGCAGGCGCGTGTCGNAGCGCANACCACCCCACCCAGCGCCGNGCCGGGCCTCCCGGNACNCCATCACCCTCAGCACCGGCGCCCGGAACAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGNCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTG SEQUENCE_INCLUDED_REGION=34,201 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1276 SEQUENCE_TEMPLATE=GTTNATNNCTCCAGGTCGACTCTAGAGGATCCCCCTGTCCAGATGGGGAGTTGGTGCATGAGTNCACAGAGGCTGGAAGNGGGNGTTGGGGAGTNGGTGGACGGTGGAACCCAGGAAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT SEQUENCE_INCLUDED_REGION=34,83 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH128 SEQUENCE_TEMPLATE=GCTTGCNTGCCTGCAGGACGTCNACTGTAGAGGATCCCCTTNAGGGTTCCNATTTAGTGCTTTACGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACANTTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATNAATTGCGTTGCGCNCANTGGCCGCTTTCCAGG SEQUENCE_INCLUDED_REGION=39,26 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1284 SEQUENCE_TEMPLATE=CTTCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTCTCCAGCAGTNGGGAACTCATACATTACACGGCAGCCCATTTCACAGAAGGATGACTTCGGGCTCACATCTCTTAGCAACTAACTGCCTAGCCCTTCCTGGCAGAGAAGTCTCAGGTGGACTCTGTCTAAGTGGGCAACACAGGAATGCACATCAGTNCACACCCTCCTAAGGAGAAGCATCTTCCCACACCTCCATTGCAGGGCTTCTAACAATTTTNTCCCCCTCCTGGTAGGCGATGCCTGGCTCCTCTCCTAAGAAATCAAAGGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTNCACACAACATACGNGCCGGAAGCATAAAGTGTAAA SEQUENCE_INCLUDED_REGION=34,271 P3_COMMENT=CL is GGGCTCACATCTCTTAGCAA P3_COMMENT=CR is TGATTTCTTAGGAGAGGAGCC P3_COMMENT=CM is TAACTGCCTAGCCCTTCCTG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1290 SEQUENCE_TEMPLATE=CTTCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTGCGCGTNGGTNGAATTGTTAATAAATTACANNCACTCTNTTCTTTGAATGTTTCTGCTCCTTTTGTATATAAGTTCCANNGGTTTCCCATGATGGATATTAATACCTACTGAAAAGAGTGACAATTGTGCTCATTGCTAAGCTGAAAAAATGTAATCCGTCTATCTGTNTTCTCTGCAGTACATAACACTAGAAAATCTAATCACCTTGAAATTGTTTGAAAGCAAGCACAAGAAAATTCATGGATGTTCACTATAGAATGAGCACAATAGAGCACCTCAGGGTNNCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCC SEQUENCE_INCLUDED_REGION=34,283 P3_COMMENT=CL is TTCTTTGAATGTTTCTGCTCC P3_COMMENT=CR is CATCCATGAATTTTCTTGTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1291 SEQUENCE_TEMPLATE=GCTTGCANNCCTCCAGGTCGACTCTAGAGGATCCCCCTAAGAAAGTNAGAATCAGGAAATATGATTTTNCTATCATGAAGGGAATTTAAATTGAGCGTGATATCTATTGCTTATAATTTTAAATTAGGCTTAATATTANGTTTTTAAAAAGTGCTAAACCACCCCTTTTAAAAACGTTTACATCTGATTCACAGAACTAAACTAAAATGTATTTGTTACAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGNGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCNAGTCGGGAAACC SEQUENCE_INCLUDED_REGION=36,185 P3_COMMENT=CL is GAAGGGAATTTAAATTGAGCG P3_COMMENT=CR is CATTTTAGTTTAGTTCTGTGAATCA P3_COMMENT=CM is GCAATAGATATCACGCTCAATTT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1292 SEQUENCE_TEMPLATE=CTNCANCCCTCCAGGTCGACTCTAGAGGATCCCTAGTNCNGANTNAGGTNGTNCCTTCAGAGCNGAGAAAGGGAGACAGGGTTACTCAGACCGGCAGTGTCACCAGTGGATGATTCCCCCNAAACACACTTCTCAACAGACTCAAGTGAGATGAAGTATACACNACAGGCAACTCGTGAATATTAACTACTTNTCTATGCTCATGCAGAATAACTTAAAATAATAAAGANTTCAACACAAATGATCAGTGACATGAATGGGAAGAGACCAATTATGATTAAACACAGCAATACTGTGAGATGAGTCAGGGGTACCGAGCTCGNATTCGTAATCATGGTCATAGCTGTTTCCNGTGTG SEQUENCE_INCLUDED_REGION=33,275 P3_COMMENT=CL is GGAGACAGGGTTACTCAGACC P3_COMMENT=CR is TTGGTCTCTTCCCATTCATG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1296 SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCTTTGAAAATGTNTTAAGTNCATTATTCAAACCCAAAGTNTTAATTTTTTTAAAAAACCAATTTTCCCATCTTTCAAATAAGGGGGATGGAGCAGATGATGTTCAAGATGCTCAAAATTCTTTTTGCTCCGTGAGATCTAAAACCTGACCTTGGCATCACGTAATGACCATCACAGTCAACACTGCTCACAACATATACAATGGGATGCCTGGTATGTTTTACAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGNTCACANTTCCACACA SEQUENCE_INCLUDED_REGION=34,223 P3_COMMENT=CL is GGATGGAGCAGATGATGTTC P3_COMMENT=CR is ACCAGGCATCCCATTGTATA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1304 SEQUENCE_TEMPLATE=GNGGCATGNNCAGGTCGACTCTAGAGGATCCCCTGTGTGACAGACCCTAAAGTGGGGAACCAGTNTAAGCCATACTCAGATTNNCAAACCACCGAAACAGTGAAATATTCAGTGTATGTNATTTTAAACTGCTGATTTTAGTGGTGACTTGTTATGCAACANTAGATAACTAATACATATATAANTGGAAACGCATACACATATGCCCTTTATGTTTGGCATACTTTTCTGGAGANTTGGAAAGGTTAAATAGGAGTTTACACCATAAACATGTAAAANNNNTGCAANTTGAATGTTAGANNTTNTNCTCAGTCCTTCANCAGGGTA SEQUENCE_INCLUDED_REGION=33,294 P3_COMMENT=CL is ACAGACCCTAAAGTGGGGAA P3_COMMENT=CR is TTTACATGTTTATGGTGTAAACTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1306 SEQUENCE_TEMPLATE=NTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGTTTCTGTACCTCACTTCTGATTTCTGTATGTCACTTTCCTTTTGTTGTNTATAAATTTGTTCTGACCACGTGGCATCCCTGGNGTNNCTCTGNATCTGCTGTGATTCTGGAGACTGCCAGATCTGCTAATCATTTTTTTTTCTTGGTCCGTTAAACTCTGTTAAATTTAATTTGTCCGAAGTTTTCTTTTAACAGGGGAAAGCATCGTATTCATAATGGCAGTAAGAACCCGATATAATTCCCTAGAAAAAAGTTATTCTCCTTTAACCCAGTAACCCTACTTCTATGACTCTATTTATGGGNAAAATCCAAAGTA SEQUENCE_INCLUDED_REGION=35,318 P3_COMMENT=CL is TCTGATTTCTGTATGTCACTTTCC P3_COMMENT=CR is TCGGGTTCTTACTGCCATTA P3_COMMENT=CM is TATAAATTTGTTCTGACCACGTGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1307 SEQUENCE_TEMPLATE=CTTCATTCTCCAGGTCGACTCTAGAGGATCCCCTGTCTAACACAGCCTATCGATATAGTNTNNTAGATCTATTTTCTGCAGGACTATGCATAAAATCCTGGGAGAGTTCATAGAAAGTNCATCTACCTAAGGCAGAAGAACAGGCAGCACCTTCAAGTNCTTCTGTTACATGGGCAATAGGATGNAGAAGTGAGCAGCATGAGCAAAGTTATTGAAGTATGCTAGAAAATAATGCTCAAGGTCTTAAGCAATTTAATAGGTAAAGGTTAGNGTNCATGNGGGTGGTAGAAGGTCAGANTAGAGTNNTAGANGNGGCATGGTAAANGNCTGGAGCAGTGG SEQUENCE_INCLUDED_REGION=33,306 P3_COMMENT=CL is ATCTATTTTCTGCAGGACTATGC P3_COMMENT=CR is CCTTTACCTATTAAATTGCTTAAGA P3_COMMENT=CM is TAAAATCCTGGGAGAGTTCATAGA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH131 SEQUENCE_TEMPLATE=TCATGCCTGCAGGTCGACTCTAGAGGATCCCCTAATAATAATTTAGTAGGATTTTTTTTTTCTTTGCCTTATGTAGACTCTGTNCTTCCTTGCAGCCCATTGAGAGCAATGATCACCATGACTGTNCCCTCACATGCAGCAGCAATGATTGTCCAAGATATACATGTTCACAGAACAAACTTTTATCATACAAGTTTTCTTTTTCTGAGAAATGTGAAGTTCCAGAATACCCTTATTTTTTTAATTTNCT SEQUENCE_INCLUDED_REGION=31,219 P3_COMMENT=CL is TGAGAGCAATGATCACCATG P3_COMMENT=CR is AGGGTATTCTGGAACTTCACA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1311 SEQUENCE_TEMPLATE=TCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTCTGGTNNNNGAGNNNNNATATATATTGAATAATAGGTTACACCTATATATTACATCTATANTATGCCAAGAAAACCTTCAGAAGTNATACTAGAGTNNATGTGGTCTGCAGCCTTGAGCAGAAAAATTGCCTGTTAATCACTGATAGTTGAATATAAGNTTAAGATACATGCATACACAATTATACTGCCAGTTTCACATATGAGCCTCTTTGTGTNCAATANAGATTGTGATGAAAATGCACAGGGTACCGAGCTCGAATTNGTAATCATGGTCATAGCTGTTTC SEQUENCE_INCLUDED_REGION=36,246 P3_COMMENT=CL is TGCCAAGAAAACCTTCAGAA P3_COMMENT=CR is GGCTCATATGTGAAACTGGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1313 SEQUENCE_TEMPLATE=GNTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCCTCAGCCAGANACCTGACTTTCTCTCCCTCACAACCACCCATGACCAAGCCTGTCAATTTCACCTCCTATTAATAATTTCCCCTGAACCAGTCCACTCCCCTCCATCTCTATCACCACCATCCTGGGGGAGGAGACCACTACCATCTCAAACCTATGTTACACACAGTCTTCCTAACTGAAATGCTGAGCCTCTTCTAAAGCACTAGAACTTTCAAAGGCTCAGTCCTACCTTTCTCACCCTCCTGCTCAAAACCTACTCCCACTGCCATTTGTCTAAACAAAACTCTTTTATGTGGCATGAAAGGCATTTCTTGATCTATCTCC SEQUENCE_INCLUDED_REGION=36,328 P3_COMMENT=CL is CTTTCTCTCCCTCACAACCA P3_COMMENT=CR is ATAGATCAAGAAATGCCTTTCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1314 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGGACATGCAGACAGTGTGTTCATTCCTTTTCATCATTGCCCTTTTAAGTCTGTTTTATCAAATACTTCTTTCCTGCCCTGGGATAAAATCTTGGGCTTTTCCAACTACTCTCAGGTATTAATGAATAGTGTGAATGAATAATCTAATTTTCCAAGCATTGAAAAGTTTAATATCCGTATCAGTTAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGC SEQUENCE_INCLUDED_REGION=36,187 P3_COMMENT=CL is CATTCCTTTTCATCATTGCC P3_COMMENT=CR is ATACGGATATTAAACTTTTCAATGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1316 SEQUENCE_TEMPLATE=NTTNCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTACGCCAACCAGGTCTCTCACCCAAAGCAAGGGGAAAAGGGCTGGATCCTGGGTGGCAAGCCAGTCCCCAAGGAAAGAATGGAGCGATGCAAATNAGAAAAAGGAACTCAAGCAGAAAGGCTCTTTTGCGGCTTCTAGTGGCAAGCCGNCCTGCATTTGCTTGTTCTGTCCCTTAGAGATGCCTCAGTTTCCTCAGCCATAAAATGGGCTGTTGTGAGGATTAAATATGANGTATAAAAATCTGTTGGATCAAGTAAGCCAAAAACATAGGGGCATTTAAAAAATTT SEQUENCE_INCLUDED_REGION=35,288 P3_COMMENT=CL is ACCAGGTCTCTCACCCAAAG P3_COMMENT=CR is TAATCCTCACAACAGCCCAT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1317 SEQUENCE_TEMPLATE=CCTCTCTCTCTTTCACACTCTGAGCCTGGCTTTTCCCAGGAATCCAATAGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGG SEQUENCE_INCLUDED_REGION=0,49 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH132 SEQUENCE_TEMPLATE=TTTCATGCTGCAGGTCGACTCTAGAGGATCCCTGACCCCTAGCCCTAGGAGCAGTGGGAAAAAAAAATACATTTTGACTGGAAGTAAACTGAGGAAACATGACATGGAGTCAGGGAGGGATTGTAGATCTTTCCCAGAGGTGTGATTTTTTAACAGACTGGCTTTTGGCGTTTGAGGAAAATCTCAACAACTCTCTGGACAGAATTTTCATAACTGTCCACTTGGCAGGGCTGATGTGTGTCTTGAAATAATTTTCAAGATTTGATTTTGAAATCTGAAATTTGATTTTATTGTATGTGCTC SEQUENCE_INCLUDED_REGION=31,271 P3_COMMENT=CL is TAGGAGCAGTGGGAAAAAAA P3_COMMENT=CR is TATTTCAAGACACACATCAGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1322 SEQUENCE_TEMPLATE=TGAAAATGTTGTTGAATTTCTAGTCTTTTGAGTNAGGAAGGTAAACTAGAAGGGNGTAGAGTNGAGGGCTAGGGAGCCTGCCCACCATGGGCACCAAACTNTTATTGAAAGAAGGATGAAATCAGTATATTTGTGGCAGGCTGAAAGAGTCAGTGGGCTCAGGCCTCATGTATCAGCAGCAGGTGAAGAACTAGGAGCACCACTGGAAAAGAGTCATCTCCTACAACTGAATCTGGGCTCTCACTCTTTTTTTGCCCTTAACTATGCTGCAGCCCACAAAGGATCTGTGGAATGGGGGTATCTTGTTAGTCAACTTGTAT SEQUENCE_INCLUDED_REGION=0,320 P3_COMMENT=CL is ATGTTGTTGAATTTCTAGTCTTTTG P3_COMMENT=CR is ACAAGATACCCCCATTCCAC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1327 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAGAAATCTGTTCTCACCCCCACTCCTCCCCGCCTCATGGTCACCCTCCACTTTCTTTGGTCTGAAACCAATTGTGTAAATTAATTCACTTCTACATTGCTAATTGCTACGGAAGCTGAGAACTCAGAGGCTGCAAATACATCTTTTTGGGTTTTTTTGCCATTTTGAAAGTACAG SEQUENCE_INCLUDED_REGION=35,178 P3_COMMENT=CL is AGAAATCTGTTCTCACCCCC P3_COMMENT=CR is TTTGCAGCCTCTGAGTTCTC P3_COMMENT=CM is CGCCTCATGGTCACCCTC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH1328 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTATGTTCACTATGGTTCTTCTACTCTGTCTTCTGTGTACGCTCATGTAGGGAAATGTTAGAATCAACATGTGACCTTTGAGATTGGTTAAGAAAGGTTGAGTCAGGGAGACATTTAG SEQUENCE_INCLUDED_REGION=35,119 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH1329 SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTTTGCTCCTGTCAGTCAGAGTNGCAGGTGCTTTTCTTTGCGTTCATGGAAATNATCCAATGGTATACACACAAGCGTCATCACTACAATTTTGGAAAAACCTGTGACTTGGAGGATTCGGGGATGTNTCATTGACTAGATGATTTGGGAGGCTCATCTGCACCCATTGGGCCTGTGCAGGTCTTGCAATAATCCTGTTGTCACCATCCAAGGACGTGAGGGCACCCTGCAAGG SEQUENCE_INCLUDED_REGION=33,241 P3_COMMENT=CL is TTTTGCTCCTGTCAGTCAGA P3_COMMENT=CR is CAACAGGATTATTGCAAGACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH1337 SEQUENCE_TEMPLATE=TNNNTNCAGGTCGACTCTAGAGGATCCCCCTTCAGTGCTGAGGAAAGAAGTGGGAGCCGTATGGATACAGCAGGCAGAGAAGCACACATCATGTCCCAAAAGGCCAGGCATGACCAAGATCTGGCTGACGCTTCCAGAGAAAACGTGCAGACCCAGGATTTTNATGAGAAATCACTAGGTTTTTAAAAATGTTGACCAATCCCTCAAAGTAAATTTAAACCAATTGTGGATGAGTATTGTGAGGGCCAAATAAAGCCTGCCTGTGGGCCAGATGGCTGAATACCCAAAAGTCTGTGACCAGCCGGATAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTC SEQUENCE_INCLUDED_REGION=29,279 P3_COMMENT=CL is GAAAGAAGTGGGAGCCGTAT P3_COMMENT=CR is TTGGCCCTCACAATACTCAT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH139 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAAAAGTNGGAAGGCAGGACTGTGTTTTGGAGGACAAAAAGTAAAATCTTTTTATATCTTTATTTTTTAATTTTATTTTTTTTCAGGCATATAGACATACATATCCATTATACAACAGAAAAGGGGGCTGGAAAAGAAAGGTCAAGTGAGATTTCAGATATTCTTAAATGCAAGGCTGACAAATTTGGGCTTGATTCTGTAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA SEQUENCE_INCLUDED_REGION=35,204 P3_COMMENT=CL is GAAGGCAGGACTGTGTTTTG P3_COMMENT=CR is AATCAAGCCCAAATTTGTCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH141 SEQUENCE_TEMPLATE=CCTGCAGGTCGACTCTAGAGGATCCCCCTGAATGCAAACATCACTGAGAAGTTTCTGAGAATGCTTCTGTTTGATTTTATATGAAGAAATTCCCGTTTCCAACGAAATCTTCAGAGGGGTACCGCGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAACTGCATTAATGAATCGGC SEQUENCE_INCLUDED_REGION=27,89 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH142 SEQUENCE_TEMPLATE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCTGTAACAGTCTGGGTTGATTTGTTTTCCGATTTTGNTTTTTTTTAAATCACAGAAACATTTAGTTTCTTCATCTATACAATGGTGATAAAAATCTGTTACATGATCCTGACTTCAGGGCCATAGATAATTTGTCCTGAAGGGGATCCCTGGCCCGCTCTGTCCTTCAATATAGGTAGTAACTGCGGAACGTGAAGATCTAGAGCATGCTTTCCTCATGAAAATGCA SEQUENCE_INCLUDED_REGION=34,227 P3_COMMENT=CL is TCTGGGTTGATTTGTTTTCC P3_COMMENT=CR is TTTCATGAGGAAAGCATGCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH150 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTTTGATGCTTGTTTGTAACTGTCCAAAGGAAACCACTGCAACCTTCAAGCATGTCTGTGTTACTCTATTTTGTTCCAGCCACCTGTGGCATTTCCAAAATATGATAATCTCTGCCACCATACTGCTTTAAACACAAATAGAATCTGGCAGCAAAATATAGCATAAGCTTACTTCTAAATCAAAGGCTACCATCAGTACCTTAGCACATTTAAAAAATAAAAACCAACACTGCCCACTTCAATGAATGCTTTCAGCACACACCACAAAGGCTACTGGGG SEQUENCE_INCLUDED_REGION=35,283 P3_COMMENT=CL is AAACCACTGCAACCTTCAAG P3_COMMENT=CR is TGGGCAGTGTTGGTTTTTAT P3_COMMENT=CM is GTGTTACTCTATTTTGTTCCAGCC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH152 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTATAATTATAAGACACCTTTATGCCCCAGGACATCCAGGTTCTTTACAGACTTTAGCCCTCTCAGGAGGCTGGTGGCATTTTGCCATTTTACAGATGAAGGTGATTATAAACAAAGCATTGAAAAGATGTATTGCACAGCAGGTTGCAGGAATTTGTGCCAGTATAAAACACCTTCTTTGTTCGCTACTAGTGTTAACATCTATAATGTGCTTAGATTGTTTTTCTCATCCTTAATACAGGGTACCGAG SEQUENCE_INCLUDED_REGION=35,252 P3_COMMENT=CL is TAAGACACCTTTATGCCCCA P3_COMMENT=CR is AAGGATGAGAAAAACAATCTAAGC P3_COMMENT=CM is GACATCCAGGTTCTTTACAGACTT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH154 SEQUENCE_TEMPLATE=GTTTTGATGCTGCAGGTCGACTCTAGAGGATCCCCTAAGAAAACAGTGTNGTAATTCCCTGGCTTTGCACATGTTCCTTCATCTCATCATCTGCCCTAAAGTNGATGCTGCTCCAATATTCAATGTGGATTTATACAAACCACTGCATGACACAGCAAACATGGTTGGACCTCATTCATATACCCCTGGCTCACCATGCAAATCACCTGCAGACAGTTCTCACACATGGCCAAGGCTTTTCTGTGTCTGTGGGCAGGCTTGGGCCATGAAGTGTGCATGTGGCCAGATCAGAGAGTTAATTCCCCAGGGAGTGACCTTCTACTACCGTGAAGGGCAGGATGTGGTAGATAAGTAGTTCAGTTTCCTCA SEQUENCE_INCLUDED_REGION=34,334 P3_COMMENT=CL is GTAATTCCCTGGCTTTGCAC P3_COMMENT=CR is TTATCTACCACATCCTGCCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH157 SEQUENCE_TEMPLATE=AGATTGNCTGCCTGCNGGTCGACTCTAGAGGATCCCCCTCTATTGATACGTGCCTACTTATTCACTTTGGTTTGTGTTTTATGTTGCTGGTATCATCATCTCTGTTTGACTCTTCTCATCTGTCTTGCCCACTGTTCCTAGTTGTCTCTCTCTTTTCTTTCCAGACTTCTAGTTAAGTATCTTATTCTAAGCATCAGATGGTAAAT SEQUENCE_INCLUDED_REGION=36,170 P3_COMMENT=CL is TACGTGCCTACTTATTCACTTTG P3_COMMENT=CR is AGAGACAACTAGGAACAGTGGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH159 SEQUENCE_TEMPLATE=CTCCAGGCCAACAGAGCCCCAGATGTGGAAGTAGAGTNNGTTGGACTGCAGTCCCACTGGCTACAATCAAACATGGCAGGCCAGATCTGTTCAGTATTAGTNCTGGNTCCAAAACCTATACATCTAGATGTNTTTCAGAATTCAGAATTATTTAGATTTTGGAAAGGTAATTTGGCATGTATGTGTGTATATTTAATAATACACTGATGGAGTCAGGGGATAGTATGCCATAATCGAACATTATTATCTTCATAGCAAATGTATGA SEQUENCE_INCLUDED_REGION=0,266 P3_COMMENT=CL is CCCACTGGCTACAATCAAAC P3_COMMENT=CR is TTTGCTATGAAGATAATAATGTTCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH160 SEQUENCE_TEMPLATE=CTGTGGGAAAAATAAGGCCTTGAAGAATGAAAAAAGTTCTATTAAAACAATTCTATTTAGGATCTGGTTACACACATTCATAAAACTAAAGATGTNNGATTCTGATTNGATTGGTAAAGTCAGTTAGNCACAAAGTAAAGAATCAAACTTAAAGGNTACAGGNCAGAGACTGAAGNTCATTGTTAAGANGGCAATGAACACCTTTAGTCTAATAAAG SEQUENCE_INCLUDED_REGION=0,217 P3_COMMENT=CL is TGGGAAAAATAAGGCCTTGA P3_COMMENT=CR is TATTAGACTAAAGGTGTTCATTGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH161 SEQUENCE_TEMPLATE=CTCTTACTAATAGGATGGGAGAGTNNGAAGGAACATCAGAGATTATTTTACAGATAACAAAACTAAGACTCAGATTAAGATGCTTCTTCATGGTTACTGTAGGATTTTAGGCACCTCACTCTTTCCAAATTGCTCTAATTGACAATTCAAACAAAATTCTACCCTCTTGTGCCTCATCCATTCATACATCCTTTCTGTCCACAACAGTGTACTTTATACATGTATATACACCCACCTACATACATATAG SEQUENCE_INCLUDED_REGION=0,249 P3_COMMENT=CL is CTCTTACTAATAGGATGGGAGAGT P3_COMMENT=CR is TACACTGTTGTGGACAGAAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH162 SEQUENCE_TEMPLATE=ATGGAGATGTTGTCACCTGGCATTATATGCTATAATNCTTGGTCACATTTATAAGTTTTTTTATTCTAATACAATGGATCTGAGTNTCATTAGGAGCCTTGCAGACAAGTGTTTCTGGGTAACTTCTCAGTAACATCTTTGACATTTGTTTAGTTGCAGACCTAAATGGCCACTGATGGATACTGCAGTTTCAGTAAGCAGTGGAGTTTATTTCCCTGG SEQUENCE_INCLUDED_REGION=0,219 P3_COMMENT=CL is AGATGTTGTCACCTGGCATT P3_COMMENT=CR is ACTCCACTGCTTACTGAAACTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH164 SEQUENCE_TEMPLATE=CTACACCACCCAAAATGAGCAGTTTCTCTAAAGAGATTGGGTTCAGTAAAGGTCAGTCCTACTTCAATTTCAGTCTGGCCCTTCTCATTCTTATGTTCTACTTAAATATTTATCTTCTATTTTCGTACCAAAATTATTAGATGTTTCTTAGTTTACATTAAGTTTCTTTTAACTGGTAACACGTGAGGATTTTCATAGACAATTTTTTCTTTTGAACAAAATTGTCAATATGTCTT SEQUENCE_INCLUDED_REGION=0,236 P3_COMMENT=CL is CTACACCACCCAAAATGAGC P3_COMMENT=CR is AATTGTCTATGAAAATCCTCACG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH168 SEQUENCE_TEMPLATE=CTTAGAAATAGAAGAGGCTTCCTCCCTCTAATGGTTCCACTCAAGTNCCTATCTGGTTCCAAGAGGTCTTCTTTTTGGATTAACAAAAATTAGTTCCAAAAGTGAGAAATTGTGCATCTTGTGGAAATGAGAGAACCACTGGCTATGCCTAGAAAATGTATCCTTGGAGGCACTAGGAAAATGGCAGTAAGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTAT SEQUENCE_INCLUDED_REGION=0,189 P3_COMMENT=CL is GAAATAGAAGAGGCTTCCTCCC P3_COMMENT=CR is CCATTTTCCTAGTGCCTCCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH170 SEQUENCE_TEMPLATE=CTCGATCTTCAGAAAGTGACTCTATCAAATCTGAAGGACACCCAAATTTAAAAAAGTATCATGTAATTAAAAAAACCACTTTGCATGAATGTATCTTTTNCACTTTAAAAAATTAAAAAAAATAGTACANNGCAGTACANACCTTTATCCCGTTCTTGTTGCTGCATTGTACTTTTCAACTTATCACTAAGNTCATTTATTATGTTTTCTTTTCTCATTTTCTCTCTTGTTAAAACAGTGTTAAAATTGG SEQUENCE_INCLUDED_REGION=0,250 P3_COMMENT=CL is CTATCAAATCTGAAGGACACCC P3_COMMENT=CR is CACTGTTTTAACAAGAGAGAAAATG P3_COMMENT=CM is CATGTAATTAAAAAAACCACTTTGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH176 SEQUENCE_TEMPLATE=CTGGTATAAAGGCCCCTTTGGATGANATTNACCCCAACCAGACTAATGGGAGTNCAGAAGGGATGCTGTGGGTCCCCCACATCNTCAGAATGCCAACGGGACCTGGGGGTCCTGTTAATCTTTTTAGCCAGACTGAATTCCATTCTGTTCTTATAAAATCCC SEQUENCE_INCLUDED_REGION=0,162 P3_COMMENT=CL is CTGGTATAAAGGCCCCTTTG P3_COMMENT=CR is AAGATTAACAGGACCCCCAG P3_COMMENT=CM is ACCCCAACCAGACTAATGGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH177 SEQUENCE_TEMPLATE=CTTTAGATATATTTAAATCATTTGCCATCAGAAGTCTGTACTGTAGGTCTTACTATTATCCTCATTTTACAGATGAAGCACAAAGATCCATAGTCACATATTTTGTAACAAGAGCAGGATCTGCAGCCAGGCAGTCTGGGTCCATGGTCCATGTTCTTACCCATGGTGCAATACTGACTCTTGAAGTCCAGGGTCTACTAAGAATCCAGGTCTACCTGACTCCAAAG SEQUENCE_INCLUDED_REGION=0,227 P3_COMMENT=CL is CATTTGCCATCAGAAGTCTG P3_COMMENT=CR is TGGAGTCAGGTAGACCTGGA P3_COMMENT=CM is ATTATCCTCATTTTACAGATGAAGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH184 SEQUENCE_TEMPLATE=CTACATTTCAAACCGGAATCATTGGCTCAGTNTGGATTCTCCAGGAAGCAGGCCTGAGGCAAAGGCTGATGTGCTAACACATCAATAGAGTNTATAGTCTTAGGGGCAACAATAGGGAATGAGGTGAGAAAGACAGGTAAGGAGACAGACTCAATAGGAAGATGTGTCATCAAGCAGGACACT SEQUENCE_INCLUDED_REGION=0,183 P3_COMMENT=CL is CAAAGGCTGATGTGCTAACA P3_COMMENT=CR is GTGTCCTGCTTGATGACACA P3_COMMENT=CM is TATAGTCTTAGGGGCAACAATAGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH192 SEQUENCE_TEMPLATE=AGTGGCCTGATGATGGCTCACTGTAACGTAACTCCTGGCCTCAGCCTCCAGAGTNNCTGGAACTATAGGTACACGCCAACACACCAGGGTAATTTTTTTTTTTTTTTTTTGTAGAGACAGGGTCTCGCTATGTTGCCCACTCTGTTCTATTTTGAAGGTACAACCAACAAGCTTTCTTGACAGNTTGAAAGTGAAAATGAGAGTATGAGAGGAGTTATGGATGATATAAAGTTTTTGGCCTGAGTAACTAGAAATCCTCGCCATCAACCTAGAATGGGGAAGACCATACATTACTTACAGGTTCG SEQUENCE_INCLUDED_REGION=0,305 P3_COMMENT=CL is GCTATGTTGCCCACTCTGTT P3_COMMENT=CR is ATGGTCTTCCCCATTCTAGG P3_COMMENT=CM is TATTTTGAAGGTACAACCAACAAG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH193 SEQUENCE_TEMPLATE=CTACCACGCCCTGCTCTCCATTCACTCTTGAACTTACTCCAAGCAGACATTTGTCCCCGCCATTCCACAAATAACCTTCATGTAGCCAACTCCAGGAGTNAATACTTTTCCTAAGTCTGTTTACTGGATTTCATCCTTTTTTCAACCTCTAAAAATTTCAGTGCCCTGGGATCTCTGGGTNTCTTTCCCTTGACAATTTCCTCTAATGCTGTGGCTTTTTAGACCACCTTTATGATGATGACATCAAAATTCCTATCCCACTTCTCCTCTGAGGTCCAGTCCCCAATCCCCACTGGAATGTTTAATAGATATATTA SEQUENCE_INCLUDED_REGION=0,316 P3_COMMENT=CL is CTACCACGCCCTGCTCTC P3_COMMENT=CR is TAAACATTCCAGTGGGGATT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH203 SEQUENCE_TEMPLATE=TGAATAACCAATCCACTCCGCTTTTAGTNGGAGTTTTAGTTGGTTAGTTTTGTCAAACCCACAATAAAACCCTGGGGTGGACCAAGAGAGTNNGCCACTCTAGTCCTTGGCTTTCTATATAGCAGGAGGTGCAGACCTTGGAATCTGGAGGAGTCTCTGGTGGCCCTCCCTTGGCCTTTAAATTTCACCCCACCCTCTCCCTCTCCTGAGGCTTTCTGGGCCTCATCTCTGTGCTGAATGCAAAAATGACCACACTTGGCATTCAAGACTCTTCATGACTTCGCTCCTGCTTACTTCTCCAGGGTAC SEQUENCE_INCLUDED_REGION=0,307 P3_COMMENT=CL is TGAATAACCAATCCACTCCG P3_COMMENT=CR is ACCCTGGAGAAGTAAGCAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH209 SEQUENCE_TEMPLATE=CTAGCACCATAGGAAGCCACTAATTATATGCGACCAAAGTGATGAACATAGCAAATAGGTGGTACCTGTGGCACATTTTAGGCATTTTAAAATAAATCTTGTGGCAATGCGTTGAAGGTTTGAATGCAAATGGATCAATTCAATATACTTNTATCTCTACAGGATAGTGGCACTTGACAGTTGATAGAAAGGTAGAAAAATTAAAATGATCATCTCTTCCATGGGCCTTCTTCTATCCCTAAGTNAAATTGATTACTTCCTGGCCACCACTGCTTTATCTTTGCGTTTATTATATTTCAGTGACCACATTATATGAT SEQUENCE_INCLUDED_REGION=0,317 P3_COMMENT=CL is CATAGGAAGCCACTAATTATATGCG P3_COMMENT=CR is GTGGTCACTGAAATATAATAAACGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH215 SEQUENCE_TEMPLATE=CTCCTGCCCTCCGACGGCGTCTCCAGGCCCCAAACGGCCTCCGGTCGGTGGGCTTCTCCAGGCCCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTTAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG SEQUENCE_INCLUDED_REGION=0,66 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH223 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTTACTACTCTCATATCTATTCANTCCCATCAGTACATAAGAAACTAGGGAAAGAAACATTNTGGTTTGAATCTGTTCTTAAGTAGGNACAATACACATGACTAATTTAAAATATTTTCCAANGTTCTTACACCCGTNATATCTAAATTTGNTACCATCANCTAAAGATTGAAGGCACTGNATTTAAAAAAAAAA SEQUENCE_INCLUDED_REGION=35,199 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH224 SEQUENCE_TEMPLATE=CTAGTTTTGTNATGTATTATTTATTTATTTATTTCTATATTTATTGCAGAGATGAGGTATTACTCTGTTGCCCAGGCTGGTCTCAGACTCCTCAGTGCAAGTNAATCCTCCTCCCTTGGCCTCCCCAAATGCTGGGATTACAGAGGTGAGACATCATGCCTGGCCATGGTTGCTGGTTTTGAAGATGTGGGAAGGGGACCATGAGCCAAGGAGTGCAGGTGGCCTGTAGAATCTGGGAAAGGAAAGGAAGTGGATTCCCCCAGAG SEQUENCE_INCLUDED_REGION=0,265 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH227 SEQUENCE_TEMPLATE=CTTTTGGTNNTTAAGCCCTGGGTNCTTTCACCTCCACAAAGATGCTTCACAGAGGTGTAAAGTGTNTTAGTCTCAATGGTCTTATACGGCGTTCAATCACATGAGACTCAAGAGGAGCAGTCACCTCAGTAATCTTAATTTTCTCTGTGCTTTCCTTCTGCTAGAAATCTTGGCCCTCCATCATAGAATTATCTGGAAAAGCCCAGGGGAGTTGAAATATGACTCAGTGCAATTCTATTTTTAGACACAGACTTAATGGTTGACTCCCATTGAAATAACTGAAATACAG SEQUENCE_INCLUDED_REGION=0,289 P3_COMMENT=CL is TCAATGGTCTTATACGGCGT P3_COMMENT=CR is TTCAATGGGAGTCAACCATT P3_COMMENT=CM is CAATCACATGAGACTCAAGAGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH229 SEQUENCE_TEMPLATE=CTAAATATTCCAGGTCAGTGAAAATGCAAAGACATTTTAGGTCACAGGAAACCACCTGGGATGTTTCAGCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCA SEQUENCE_INCLUDED_REGION=0,72 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH232 SEQUENCE_TEMPLATE=CTACTAAGCAAATTCTGCTCAGCCTTCTGCCTTTCCTGACTGTCTTGTTGGCCCTTCCCACTTTAAGGATGCCTGTTTAAGTAGCCACCTCTAATTAGGAATCTTCCCTTGTTCTTTCTCAGGAGGCTTAGACACTGTCAGTTTCCTGAAGACAGAAAATAAGCCTGCATTATCCTAGTAGTGGATTCAAAACTAATTGTGTCCTGAGTCTTTCAATCATCAAGACATGGGAAACACTCAACAG SEQUENCE_INCLUDED_REGION=0,244 P3_COMMENT=CL is TTCTGCCTTTCCTGACTGTC P3_COMMENT=CR is TGTTTCCCATGTCTTGATGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH233 SEQUENCE_TEMPLATE=CTTATTTAAATAAATAATGTCTGAAAACTTCCCAAATCTTGGTAGGAATATGGCTATCCATAATCATGAGGCTCAAGCAACATTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGNGAAATNGTTATCCGCTNACANTTCCACANAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTANTGAGTNAGCTAACTCNCATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCA SEQUENCE_INCLUDED_REGION=0,86 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH238 SEQUENCE_TEMPLATE=AAAGACTACCCATAGTATGAGTCAATTTATTTTAAAATTACAGAAGATGCTAAACTAGGAGCAGCAAGGAGATTAGTACTTTCAGAGATCAGGGCAAGGGAAGGGGCTTGACTGCAAATGGACACAAGAAAACTTTTTAAGTAACGAATATGTTCTGTATCATGATTCTGATCATGGTTTAANTACCTGTCAAAATTCATCAATTATACATTTTAAATTGGTAAACTTTATTCCAAGTAATTCATACCT SEQUENCE_INCLUDED_REGION=0,249 P3_COMMENT=CL is AAGATGCTAAACTAGGAGCAGC P3_COMMENT=CR is GAATTACTTGGAATAAAGTTTACCA P3_COMMENT=CM is GATTAGTACTTTCAGAGATCAGGGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH240 SEQUENCE_TEMPLATE=CAGATCTGTAATCTGCTTTCTAGATCCCTTTCTTACCCATTCTTAAGCCATCACAGCAGAGTACCTTTCTAACTTATAAGATTGTGTAGAGGTTGGAAGGAGGACAGGACTGTTCTGTTGGTATAATGACCCTGTGTCCAGTTAATCCATG SEQUENCE_INCLUDED_REGION=0,151 P3_COMMENT=CL is AGCCATCACAGCAGAGTACC P3_COMMENT=CR is TGGATTAACTGGACACAGGG P3_COMMENT=CM is GTGTAGAGGTTGGAAGGAGGA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH243 SEQUENCE_TEMPLATE=NGGNNNCCNNCAGNGNCTGAAGGAAAGNACTGGCTTTTGGGGTACAAGGGTGACTAGAACCTTNCTGGGACTNTGGCTATAGTACTTTGAAATTATCACCCATATAAAAGAGGGACATGGACTATAACGTTGATTTCTTATTGCTGTACATTGCGTTTTTAACCTGCAAGTTCTCAGTTTCTTCACCATNACTCCATCAAAGNCCCTGGCTATAAGATCTGGATTTTACCCACTCCATCTTCTCTTTCCTTCTTACTGTGTNAATTCCTATTTTCTTTCAGAATCTTCTAAAAACAGTTGTATCTAACCGCATGTGCTGTTGTG SEQUENCE_INCLUDED_REGION=0,324 P3_COMMENT=CL is TTGGGGTACAAGGGTGACTA P3_COMMENT=CR is TGGAGTGGGTAAAATCCAGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH245 SEQUENCE_TEMPLATE=AAGCACAGTGGATATTTTTAGGCCTAGCATTCTTTATATTTATCCACTTAAAAATATATTAGTTTCACCTAGATCTAGGTAAAATAGTTTGCAAAAAGATTTTAGAATTGGGCAATTAATTTTAATTCTGACATCTTGGGCTTTTCACTTTTNCTTAGGCTCTCCTCGCTGACTTCGTTATTTACTTCATCTAGCATCCATCTTAACTTCTGTGTGAGCCCCTTGCCTTTGAAAACATGTCTTACTTTGT SEQUENCE_INCLUDED_REGION=0,250 P3_COMMENT=CL is AGCACAGTGGATATTTTTAGGC P3_COMMENT=CR is GGGGCTCACACAGAAGTTAA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH250 SEQUENCE_TEMPLATE=TGAATCTAGTTCAGATAAAAAATGTGCTCAACCTCAGATAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=0,41 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH268 SEQUENCE_TEMPLATE=GCTCGCATCCCTCCAGGTCGACTCTAGAGGATCCCCCTTCATTTAATCAAAAGTTTAAAATTATAAGCATTAAAATGGCTGATAAATCACCTGCTAAAACCCTAGGAAAAAAATCTGCAACATGGAACAGGGACAGAAATCGTTAACTAAAGAGAAGAACTGGAAATTAAAAATGAGCATGAAATATGGTAGGATTAATAAANCAATAATAAGATAATCAGCAAGTTGGAAGTGAAATGGGATAATGTAGAAATTATGTTAGGACAGAAATTTACATCCTTATAACAG SEQUENCE_INCLUDED_REGION=35,253 P3_COMMENT=CL is TGGCTGATAAATCACCTGCT P3_COMMENT=CR is AGGATGTAAATTTCTGTCCTAACA P3_COMMENT=CM is AAACCCTAGGAAAAAAATCTGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH271 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTATTTTAAAAAAATTCTTATTTATTTATTTATTTATTTATTTATTTTGTAGAGANNAGGTCTTGTTACCTACCAGTCTGATCTTGAACTCCTGGCCACATGAAACTTATTAAAGGAAGTGACCATAATACTTTGTACAGTAGCATAGCACATTTATAATGCACATTGAACTTCAGGGGAATATATGAAAGTAAGAAGAAAATTTGCTTAGAACTTTCCTGCAGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCC SEQUENCE_INCLUDED_REGION=35,224 P3_COMMENT=CL is AGGTCTTGTTACCTACCAGTCTG P3_COMMENT=CR is TAAGCAAATTTTCTTCTTACTTTCA P3_COMMENT=CM is TCTTGAACTCCTGGCCACAT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH274 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCAGTCACGTGTCACATGCAGTGTTTGCACTTGCCATTCATGGTTGTGAGGATTAAACAGACAAAAAGTNTACATAAAGAACCTAGCACGGCCACTGGCACGTAGTAGGAACACAAAAGAAAATGGTATTCCTTNTGATCTTTNTTATTTNATTTAGTCCTCATAATGCTTNTGTGNCAGCATTATCACCATTCCTGCACGAGGAAACAAGGCTTCAAAAGGATCAATAATTATGTAAACCCAAGGCTTGTGGGTGGCAGAGATCCGGCCGGAATTCA SEQUENCE_INCLUDED_REGION=35,281 P3_COMMENT=CL is CACATGCAGTGTTTGCACTT P3_COMMENT=CR is TTGATCCTTTTGAAGCCTTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH286 SEQUENCE_TEMPLATE=CTTCTTTTAAAAATCCTGTCAGCTGCAAAGGAAAGGAAGGACTAATATTTTCCACGAGATCAGTAACATCTTTATGAAGATGCTCCTTTTGAATGTGGTACAGAAAGTTGGAAAACACTTAGGCATGGTATCTGGCATAGTGCCCCTTAGGGAGCACTTCACATGCAGACGGTCTGGGGCTTAGGATGGTTTGACTTAGAATTTTTTGACTTTATGATAGTTGCAAAGGCGATGCACATTCAGTAGAAAGTGAACTTTGAGTCCCCATAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGT SEQUENCE_INCLUDED_REGION=0,271 P3_COMMENT=CL is AAATCCTGTCAGCTGCAAAG P3_COMMENT=CR is TTCTACTGAATGTGCATCGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH288 SEQUENCE_TEMPLATE=CTCCACTTCCCGGGTTCNTGCCATTGTCCTGCCTCAGCGTCCTGAATAGGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG SEQUENCE_INCLUDED_REGION=0,49 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH293 SEQUENCE_TEMPLATE=CTACTGGAGAGAGAACTGGGATTCAAATCCAGGAGCCCGCTCTTCACTCCAACACTATATTGCTTACTTAATGGTTACAGATTAAGCCCAGAAAGGAAGCCTGTCTCAATACACTAGATATAGTTACTGTGATTATATATTTTAATAAATGGTCCTTTTATTAAAAAAAAAAAAGNTATCTAAAGAGAAAACCATAATAATCTCTCAGGTAATTATGGCCACAGCCAAAACCAGTCTTTCTAAACCTAAAGACTCTCATAAAGGCCCTATCACATAACTTCTCCACTTCCCAGTAAAGCATTGAGG SEQUENCE_INCLUDED_REGION=0,306 P3_COMMENT=CL is CTCTTCACTCCAACACTATATTGC P3_COMMENT=CR is TGCTTTACTGGGAAGTGGAG P3_COMMENT=CM is TACTTAATGGTTACAGATTAAGCCC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH295 SEQUENCE_TEMPLATE=CTGTGACCAATTGTTATTTTAGAGGGTTTAACAATGGCCTGACTATCACCTGATGGTCGCCAGAATTTCCTGGGGGAGGGCCTCCCCTGCCCTGATCATGTCTACCTAACTGCCTACTCTAACAATACTACTCCTGTGGTATGGGGATCCTAAGCCAAAAAGCTGAAATGAACATGTTCTAGCACTACAGAAATCCATACTGCCCCTCAGTAAAGGCAAATTTTAAATCTCTTTGGATAACCCAGGGCACATGTGAACAACCCCTTCTGAAGAAGCACTTATTCTTCTTCACACTCACCATGAAAA SEQUENCE_INCLUDED_REGION=0,306 P3_COMMENT=CL is TGTGACCAATTGTTATTTTAGAGG P3_COMMENT=CR is ATGGTGAGTGTGAAGAAGAATAA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH303 SEQUENCE_TEMPLATE=CTCACCATTACTTGCTCCACTGGGNCCCTGCAGGNATGCTGTGGTCCTCCCACTGGCAGGCTTCAGGGTACNGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCANCTGCATTAATGAATCGGCCAACGNGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCA SEQUENCE_INCLUDED_REGION=0,65 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH315 SEQUENCE_TEMPLATE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCCTAGCCATTAGGACTTCCTGAAGACAAGGCAGGCAGAACAAAAGAGAAAGAGCAAAGGTTCACAGGCAAGAAAGGACCTAGCAGCATGTCCAAGAAACAGNAAAAGGCTTGTGTGACTCAAGTGGGAAATCATGGGAAACAGGTCTGATATGAGGCTAAGGATCCAGGCAAAAGCCAGACTGTAAAGGGCAGT SEQUENCE_INCLUDED_REGION=31,193 P3_COMMENT=CL is GACTTCCTGAAGACAAGGCA P3_COMMENT=CR is TTTACAGTCTGGCTTTTGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH318 SEQUENCE_TEMPLATE=TTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGGTTATTTTTCAGATTTGTTCATGTAGTTGCTTCGTAATGTCATTGGTATTTGCACTTAAGCGGAGGTAGTGGTTTTTCCTTTCCATATTTATTCATATTTATTATTTTCCTTTTTTTTTAAGTCACACATGAGTTTTTATTAAAGNCATATACTTACATTTTNAAAGCAAATATATTGACTAGTTCTTCTGAAAAATTGCTTGAAAAATGTAATTTTTTTT SEQUENCE_INCLUDED_REGION=32,224 P3_COMMENT=CL is TATTTGCACTTAAGCGGAGG P3_COMMENT=CR is ACATTTTTCAAGCAATTTTTCA P3_COMMENT=CM is AGTGGTTTTTCCTTTCCATATTT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH319 SEQUENCE_TEMPLATE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCTGAGAATCAAATCAAGAACTCAACCCCTTTCACAATAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGNGCGGGGAGAGGCGGTTT SEQUENCE_INCLUDED_REGION=31,37 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH323 SEQUENCE_TEMPLATE=TCATGCCTGAGGTCGACTCTAGAGGATCCCCCTTATTTTGAAAAATAGTTCATATATTTATAAACATCTTTTAATTCCATTTTTCCATGAACTTTTTGAACTCCTCATATTCTCTATATCTNTNTTTAAAAATTTACAAAAATAAAAACTTTTAAAAGTTATAAAAGAAATATTTTCATGTCAGATATAGGAAAAAATACAACANGGTCACCTAGAATTTTACCAGAACCACGTAGCAACCTCTAATCATTTTGTGTGCTTAATTTTAGTCATTTTTATTATTCTTAAGATGTTAAATAATTTAAA SEQUENCE_INCLUDED_REGION=31,275 P3_COMMENT=CL is AACATCTTTTAATTCCATTTTTCC P3_COMMENT=CR is CAAAATGATTAGAGGTTGCTACG P3_COMMENT=CM is TGAACTTTTTGAACTCCTCATATTC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH328 SEQUENCE_TEMPLATE=NCTTCATGCTNAGGTCGACTCTAGAGGATCCCCTAATTAACATATGCATTATCTCACTTATCTCTTTTTTGTGGTGAGAACACTTAAAATCTAAGAATGATCAATTTCAAATAAAGATGGTAGTGAGCGAACAGAAGAGGTTTCATTGACTCCTAAACTGAGTACTCAAAAACGAGCAGGTGCTCACAGTCAGGAAGCAGGTGCTGAGTACAGGATGGGAAAAAGCATGGGGGTGTGCACTTGTCAAGAAAATGACAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCG SEQUENCE_INCLUDED_REGION=33,190 P3_COMMENT=CL is TCACTTATCTCTTTTTTGTGGTG P3_COMMENT=CR is ATCCTGTACTCAGCACCTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH331 SEQUENCE_TEMPLATE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCCTATACATTTNAATATTGACTGCAATTGCACGTCCTATATCAATACTCACCTGTGTTTTTTAAACTGTGAACCAGTAAAGCATGATGATGATTGGCATCCCTCTGCAGTTAAAAAGAAGCCTAATAGTGTTAAAAATTCAATTCATCCAACACAAAGTTCTAAAATTTTNATGCCTTATAGAATTACTTGAGCACTCTCCAAAATCCCATATCCATGTCACAACCAATATTAAGTCCAAATATAACCAGTCCTTTTCTTTTTAATAGTCTTCATTTTTTTANGAGCAGTTTGAAATCCACAGCAAAGGGGTACCGA SEQUENCE_INCLUDED_REGION=31,275 P3_COMMENT=CL is ACTGCAATTGCACGTCCTAT P3_COMMENT=CR is AGACTATTAAAAAGAAAAGGACTGG P3_COMMENT=CM is TGTTTTTTAAACTGTGAACCAGTAA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH333 SEQUENCE_TEMPLATE=NNCCANNNGGTCGACTCTAGAGGATCCCCCTAATCCTGATCTATTCAAATCTTAACAACTTAAGTTGATGGAGTNGACTTCTTCTAAATTAGTNATTCCCTAATTTGCCTGACTGTTGGAATTTCCAGGGCATGTTGAAAATACACATTATCCAGACTCTTACCTCTGCAGATCTATTTAGTGGTTCTAAAATGGCAGCCAGGAGTCTGGATTTTTCCCAGGGGTCCTGTGTAATTCACACTGATGAACAGGCAAGTTTGGGAAATAGTGCCTTAAGGAGATTTTTCATTAAGCAGTCTTCATTTGAAATGAGG SEQUENCE_INCLUDED_REGION=29,285 P3_COMMENT=CL is ATCCTGATCTATTCAAATCTTAACA P3_COMMENT=CR is AAGACTGCTTAATGAAAAATCTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH335 SEQUENCE_TEMPLATE=NATGCTGAGGTCGACTCTAGAGGATCCCCTCAGATTTCTTCTCTACGTTGGTTAATGAGTNCCTTTTCAAACGGTTATATTTTGGGGTTGAGGTAAAGGTTTTTAGAGTNNTTCCAATCGTGTATGTAAGCAGTCAACTATTTNCCCTATGTGTAGGTCTGGCACCACAGACCTGTAGAAGTCTTCTCTTTAATTGTACCATTATTTTGAATGTAAAAAAAAANCAANTAAACATGTTGTTGTCATTGANCTGGCTGTAGATGCATACATAAG SEQUENCE_INCLUDED_REGION=29,244 P3_COMMENT=CL is TCAGATTTCTTCTCTACGTTGG P3_COMMENT=CR is TTCTACAGGTCTGTGGTGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH336 SEQUENCE_TEMPLATE=GCCTGCAGGTCGACTCTAGAGGATCCCACCTATCCCATTACGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=27,14 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH341 SEQUENCE_TEMPLATE=CGTTCATGCTCAGGTCGACTCTAGAGGATCCCCCTCCTTGAGGNCAAGTACAGCATGAAGGAATAATACTGTGGAAGTATAAACAGCAGGCTCCAGGCTCATCAGAAGAAAGCCTTACTGTGTACTTCTCTTTAAAAAAAAATATGTGAAATCAGATATTTATAGGAATTAGATATGTATTTNCATATTGATACATTTTGTGTATTATATGGGATTCCTGATATATCTATGTAGTATGGTTTCTNATACATAANNTATCTGATTTCATATGT SEQUENCE_INCLUDED_REGION=33,239 P3_COMMENT=CL is ACTGTGGAAGTATAAACAGCAGG P3_COMMENT=CR is ATCAGGAATCCCATATAATACACA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH343 SEQUENCE_TEMPLATE=GTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTATCTTGTNTGCTCCTTACTGTACAGGTGGCTGACAAAGAGAAGGGAAGATGGAGCCGCCATCTTGAACATGATTGACGCAGGGTNCCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCANCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGC SEQUENCE_INCLUDED_REGION=33,82 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH345 SEQUENCE_TEMPLATE=TTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTTCATAACTAGTNAGGGCCTTCATTCTATCTGAATAGATTNTAGCAAACCATCTATTCCATGCAGATAAATTCAGTCAATTAGGGTCCGNGCTCGAATTCGTAATCATGGTCATAGCTNTTTCCTGTGTGAAATNGTTATCCGCTCACANTTCCACACAACATACGCGCCGGAAGCATAANGTGTAAAGCCTGGGNTGCCTAATGAGNGTGCTAACTCACA SEQUENCE_INCLUDED_REGION=32,83 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH346 SEQUENCE_TEMPLATE=GTTCATGCTCAGGTCGACTCTAGAGGATCCCCTCATTGTGTTATGGTCAAAAAAAAAGTTTTTCAGGGGCAAATATGTGCTTCATTTATGGTAAATTTGTGTTCTCCAACTTTATTTACTTAATGTATTTCTNGTTCTTCTGCATATACAATGNNNTATCATTTCAAGGCTGCTTTGTAGTGTAATCTCTATGCANACATGTTAAGTAAAGCANATTAAGATGCACCATGTATTGTGCAGTCAGTGCAAATAGCCTAGGTGACAATCTGNTGANTGGNTAGATGTCTTCCACCTTGGGCCAACTTCACATANTTGTAAGGGAAGACA SEQUENCE_INCLUDED_REGION=32,295 P3_COMMENT=CL is AAAGTTTTTCAGGGGCAAAT P3_COMMENT=CR is AGGCTATTTGCACTGACTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH347 SEQUENCE_TEMPLATE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTCTATCTGTNNATCTTGCCTCTGAAGTTTTGACGNCTTATGGAGGTACTTGTCTTAAGTCTAGATTTGGGGTNTTCAGAAAATTGCTGTTTTTCTTTTCTGAAGCAGGCTACATGAATGATTGGAGTAGATAAAAAATCACTNTCTGCACAATNTTTTTTGTAAATTAATTAATGTATGGAGCCATTCTAATGATAGATAGGGAAATTATTTCCACTGTAACTTGAAGAACAAAAGCTGCCGTATTTTAAGTTAAAGAGGTCTAAAATT SEQUENCE_INCLUDED_REGION=31,270 P3_COMMENT=CL is TCTTGCCTCTGAAGTTTTGA P3_COMMENT=CR is GACCTCTTTAACTTAAAATACGGC P3_COMMENT=CM is ACTTGTCTTAAGTCTAGATTTGGGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH348 SEQUENCE_TEMPLATE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTCACACTGATACATCCAATTCAAATTTAACACCATTGGGTATATTATAATTTTNGCTCTATCCATAGTTCTAACCCTCTTCTCTGACAGTGAGACACCTGCCTTCTATTGTCCTTGACGTATTAACGTATTCGATCAGTCACCCATCTGGAACCAAGGTTTCATTTCTGCTGACCCCTCCCTCCTCACCCTACTTGGGCTCTGACTTCCTTTCCTGGGCTGAACCTTCTCTGTGTGGCTGTCCGCCTTCCTCTGCTTGGGCTCCAATACCCTACATGGGTGTCTGCTCATCCTGCGCAGAGGGGTAC SEQUENCE_INCLUDED_REGION=31,308 P3_COMMENT=CL is TTCAAATTTAACACCATTGGG P3_COMMENT=CR is CACCCATGTAGGGTATTGGA P3_COMMENT=CM is GCTCTATCCATAGTTCTAACCCTCT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH354 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCCTGAATTTAAAAGTNGCCAGTTATTCAGAACCTTGAAAGCACCGTGCAGACCAAGCCAAGGCGCATCCGGTGTGTGCGTCCTCTCCGGTTGGTGGTCTCACTGAGGATGAGGCTGGGGTTTGGATCTTGTGGAGGCAGAGCTGTGTCCTGAGCACAAGAAGGCATGGGATGGGAAAGGCCATTTTCAGCAGCATTTGATGGCATCGAGGTTAGCCAGGCCAGACGTAGAGGGCACTCCCTTTGGGGTCCCCCTTGTTACTGGGGCCCTCT SEQUENCE_INCLUDED_REGION=36,272 P3_COMMENT=CL is CAGTTATTCAGAACCTTGAAAGC P3_COMMENT=CR is AGGGCCCCAGTAACAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH356 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAATAATAAGGAATTACTTAGTCAAGATAGTTCTTGATCGGTAAATTTTGTCTCACGTACATAGCAGTATATAAAATTCAATTTTTATTTTAGTTAAACTAAGTNTCAAATGACCACAGTGACGATTCCAGCACTGGATCCTGTATTCCCTGGTATTCTATTAGGTCATATTTCAACTACTGTAAATATGTAAATTCTGTTACACAGAAGATCTGGAAATAACCAAATATTCTTTTAAAAGTCAAAACTGTTAGCACTTCAAACCCATTTACTAAAAACTAGAGT SEQUENCE_INCLUDED_REGION=36,288 P3_COMMENT=CL is TTAGTCAAGATAGTTCTTGATCGG P3_COMMENT=CR is TTTAGTAAATGGGTTTGAAGTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH362 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTGGCTTTGGAACTAAGCCTGAAGGTCAAATAGGGTATCCATAGCAGGGGTCTGGGGCACTAGACAGTCTGTTCATTTTCAGGAATATATGGGTCANTTTTGTATGTGTGACCT SEQUENCE_INCLUDED_REGION=33,113 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH364 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAGGCTTCCTTTCTCTACAAGGAGGAAATTGAAACCATTTGTGAAAGAGCATTTCCTTTTCAGAGTCTTATCCTAGTACCTCCGAGAACTCTGTGAGTCTGTTCATAGACGGGGAGGGAGCAGGAGAAAAGGCTGAGGGCTTTTTAATACCTGGGNCAAAAGAATCTTTCCCTTAAAATAAAAAATGC SEQUENCE_INCLUDED_REGION=36,190 P3_COMMENT=CL is TAGGCTTCCTTTCTCTACAAGG P3_COMMENT=CR is CCCAGGTATTAAAAAGCCCT P3_COMMENT=CM is GGAAATTGAAACCATTTGTGA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH367 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTCGGGAGGCTGAGGCAGGAGAATGGCCAGAACCTAGGAGGCGGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTT SEQUENCE_INCLUDED_REGION=36,49 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH373 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTATTCTCTCTCTGTTTCACTCTCACTTCCCCTTTCTCTGCCTTGAATGCCTATNATATATTGGCTTTACTTGGATTTCCTCTTTAAATTGAAGGTGAATATAATGTCTTTACTTTTTGGGTTATTGGCTCTGTAGATGTTCCTCTTCAGTTGCAAGGGACAGAAACCAATCTATTTTTCCTGACAACCTCTGAAATAAAAGTATGGAAAAGTTAAATGGCTTTAATATAAG SEQUENCE_INCLUDED_REGION=34,235 P3_COMMENT=CL is TGTTTCACTCTCACTTCCCC P3_COMMENT=CR is GTTTCTGTCCCTTGCAACTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH375 SEQUENCE_TEMPLATE=CATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCTAGCATTTAGACCTGGAAGCCTTTCTGCTACCCAAAGTGCCACGTGTTGGGTTGAAGCAGGCTGCCTCAGGACAACCAGTCACTCGGAGCACTTGCCTCCATATCCAGGCTAACCAGTGTGTTGGTATAAGTGGTTTTCAAGTAGGG SEQUENCE_INCLUDED_REGION=31,151 P3_COMMENT=CL is CTTCTAGCATTTAGACCTGGA P3_COMMENT=CR is CCTACTTGAAAACCACTTATACC P3_COMMENT=CM is GCCTTTCTGCTACCCAAAGT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH378 SEQUENCE_TEMPLATE=CATCTGGGATTTTAGTTTTTACTATTAATATTTGTGGGAACATTTCCGTCATTAAAAACTCCACATCATCATACCATATGGTGTAATAATATCCGCTTGTNTGGGCATACCATGACTTAACTGCTCTCCTATTGTTACATGTTTAGAATGTTTCCATTTTTTCACAATTAAAAATGGTGCCATCTGTGCTCATATCCATGATTATTTCCTTAAGTAAGTTCCAGAAGTGGAATTGTTGTATCAAGGGATTTGACAAATTCTAAAGCTTTCG SEQUENCE_INCLUDED_REGION=0,271 P3_COMMENT=CL is CATCTGGGATTTTAGTTTTTACTAT P3_COMMENT=CR is AATCCCTTGATACAACAATTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH379 SEQUENCE_TEMPLATE=GTTTTGCGGGTTAGAGAGCGGGGCGTGGGGGCGGGGAGGGAGAGTAACAATTTTGGAAGTCCCTGCGTGGTATCTGGAAAGTTAACGACGCTTCAGGTTCTAGGTTGAGCCCAATGACCGTCTTGCCNNGGCAGGGTACCNAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTNAAATTGTTATCCGCTCACAATTCCNCACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT SEQUENCE_INCLUDED_REGION=0,133 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH385 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTGAACAAATGCAGGGGTGTGGGTTGGAGTGGCGTGTCTCTACTAAAGGCTCCTGGGAAGGAGGGGTACCGAGNTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGNTAACNCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA SEQUENCE_INCLUDED_REGION=35,63 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH396 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTATGAGATTCAGTGTNTTGTCATCTATCCCATCTCATCACCCACCCTTCTCTAACTTGGTTACTCTTTTCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT SEQUENCE_INCLUDED_REGION=35,76 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH399 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATATTACTCCCTATAATTATAACAGACTACCAGACTGGATTTTAGGCTTGTCTGTTCCACTAAAGAACTGGTGACTCTGGGACTAGAATTTGTAAGATCAGTGTAGACTAGGATGGGATTTACAATTCTGGTGCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGA SEQUENCE_INCLUDED_REGION=36,138 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH426 SEQUENCE_TEMPLATE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCGGACATGGTGGTGGGCACTGTAGTCCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTNAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGC SEQUENCE_INCLUDED_REGION=35,29 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH429 SEQUENCE_TEMPLATE=CTCTATACATATTTAAAATTTTTNTTGTTACAAAGATAACTGTATCAAGGTAGAGGNTAAAACATATTTNATTTAACTTTTTGTTGTCATTGTTGTTGTTGTTTTGAGATGGAGTNTCGCTCTGTCGCCCAGGTTGGAGTGCAGTGGTGTGATCTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG SEQUENCE_INCLUDED_REGION=0,158 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH436 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCAGAAAACAAAAATTTCGTACAGTNCAAATAACATTTGGGAATTCCTTAAGTNACCCATAAAGTACAATATTCGATGTTTCGTGTNNACAACCCCAGGAAAAATGTTTAATGCCCGTAGTAATGTACCTGATAGGAAAAAAAGTGCATATGCCAAAATAGAATGTTTGATGTAAAGGAGAGACAGAGAGAGGAAAGAGAGAGAGAAAGAGAGAGAGACGAAAGAGAGAGAGGAAG SEQUENCE_INCLUDED_REGION=34,236 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH437 SEQUENCE_TEMPLATE=TGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAGGACGGGCAAAGTTGAGTGGCTGAAAGTGTAGGGAACAGCATTTCATGCAGAGGGAATTGCATGTCCAAAGCCTGGCATGCAGCAAGC SEQUENCE_INCLUDED_REGION=37,93 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH441 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATACTAGGAACTCAGTTCCTTTTTCTTAAAACAACATAAGACAGGAAAACATCACATCCTTTAGGTNNCGAAGACTGAGCCCATTTTAAACGCCGTTATTTTNCCCANCCCACCGNCCCAAGCCCCCCGCCCCCCGCCCCGAGCGAATGCTGTTATAACTGGTTTATCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAG SEQUENCE_INCLUDED_REGION=36,134 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH459 SEQUENCE_TEMPLATE=AGACAACCCAGACAGTGAGACACTAATCAGGAGTCTAATACAATACAAAAGAGCAATGAGCATATGAACCATCATATGCTGTTATCATTGAGCCACTTATACCCTGCATGATCAAGCACCCAGTGCCCTTGTAGTCTACATCCCAGTACGAATAAAAGCCAGAGTATTGACTCTCTTTAAAAGGTAAATTTTAATTTTTTCCTTACCACTAAGTATAATTCAGATAGCCCAGG SEQUENCE_INCLUDED_REGION=0,233 P3_COMMENT=CL is CAGACAGTGAGACACTAATCAGG P3_COMMENT=CR is AGTCAATACTCTGGCTTTTATTCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH465 SEQUENCE_TEMPLATE=CGNNNTTGATGCTGCAGGTCGACTCTAGAGGATCCCCCTATGTGCAAATCAGACCACAAGAGAGGANGGAAATTGAATCAATGGAATGAGAATTATTTGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCC SEQUENCE_INCLUDED_REGION=37,64 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH471 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTGCTCTGATTTTACCTCACCTCTAGAGTNNGTTTTATATGTAGACTACTTAAGGCACTTCCTTCGGACAGTGTTGGGAGAGACAGTGATAGATTTTTTTTCAGATGCCTGACTTGCTTTTTTTCAGATGTCATTACANTGTACTGAAATACTTTTTTCAAATACGTAATAATTCAGATAGATAACCATGTGTGTTCAGGGGACCCCCAGATGATCCTGAGGTTCAGTGACTCACCAGAAGTACCCACGGAACTCAGCATATAGAAACATGTTTTAGTTTGCTGAACTTTTATCCTGTTATTTTACTGAAG SEQUENCE_INCLUDED_REGION=36,313 P3_COMMENT=CL is GTGCTCTGATTTTACCTCACC P3_COMMENT=CR is TCTATATGCTGAGTTCCGTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH476 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGGCCCTTCCCGGCATCAACCACCAGGCCCGAGTGTGGGGCCCGAGTTGCTGGCTGCCCTGAGTGCCCTGGTCCCTGGGGCCCCCATGGGAGATCAAGGGGCTCAGGAGAGGGCATGGGACTCAGGAGGTCCCGGGGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTNAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG SEQUENCE_INCLUDED_REGION=36,141 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH479 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGATTTCAGAATCACTATAGACAAGTGACCGCTATTGTTTCCTCTTCTCCCCTTTATAAATGGGAGAATTTACTATCCTACGCCTATTTCACCATTGTATATTGGTTCTACATGAGTAGATCACGTGTCTCTTTAGTTCACAAGTCTTTAAGAGGAAATGCACTTGAATATGGGCTGGACTTTGTGATTTGCTCCATCATGAAAATGTAGAGGAAGTAATATTCTAGGACTTCCAAGGCTAACTTATGAAAAGCCTTGCAGTTTTTGTCTGGATGTCTGCATCTCTTGGCATGTTTCTTCCTTAGCAACTCCC SEQUENCE_INCLUDED_REGION=36,315 P3_COMMENT=CL is TTGTTTCCTCTTCTCCCCTT P3_COMMENT=CR is GCTAAGGAAGAAACATGCCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH480 SEQUENCE_TEMPLATE=CAGTAACTTTTCATAAAGCAACCTCACCTCTACCATTATGGTGCTCACTGTCTGAATTATCCTCCTCTTCTAGAAACTATATTTACAGTTTGTCGATGATCCTGGAAATGACTACTCTGTTAAATCTGTGAAACTGATCTTGAGGTGTTTTATTTATTTATTTTTTCTATCAAGACTAAAATTTCCCAGAGGGGTACCGAG SEQUENCE_INCLUDED_REGION=0,126 P3_COMMENT=CL is AAGCAACCTCACCTCTACCA P3_COMMENT=CR is GTAGTCATTTCCAGGATCATCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH481 SEQUENCE_TEMPLATE=AATGAAAGAAGATCTGAGAGACATGTGATCTTATTATGTTCGTTGTAAATACACAGAATACTGGAAACAACATAAAGCCAAATAAAAAATGATCATTTTTATAATGTATGGAATAACTGTGCAATTTTTCTAGTACAATAAAATAATTTAAATCTATATTGTATGATTGCATGGCAGTTCACGATATATTAATAAAAA SEQUENCE_INCLUDED_REGION=0,198 P3_COMMENT=CL is GAGACATGTGATCTTATTATGTTCG P3_COMMENT=CR is ATATATCGTGAACTGCCATGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH484 SEQUENCE_TEMPLATE=TCCATTAAAGAGACATAAGATCACATCACTTCTGTGCTCAAAACCCCACAATGACTCTCTATTTCCCAGAGGGAAAGCCAGTCTTTACCAGAGTCTACAAAGCCTACACACTCGATCCCTGTTGTTTCTTGACCTCATTTCTTACATCTTCTCCAGGNCCACTCCACTCCAGCCACACAGCCTCCTTGCTTTTCCTTGAAAACACCAGGCACTCACACACTGCGAGGCTTTGAAGTGGCTGTTCTCTCTGCCTGGAATATTTCAATACATGTATCTGCATAGGGGTACCGAGCTCGAATTCGTAATCATGG SEQUENCE_INCLUDED_REGION=0,162 P3_COMMENT=CL is CCATTAAAGAGACATAAGATCACA P3_COMMENT=CR is TGGAGAAGATGTAAGAAATGAGG P3_COMMENT=CM is CACTTCTGTGCTCAAAACCC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH486 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCAAAAAACTTGATTTGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=36,15 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH488 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAGACATTGTTCTTACCCTAGATCATTGTGATTTACAGAGAAAGAGCAATATAAGATCAATTGTACAAAACTCAAATGTTAGGAATTTCTCC SEQUENCE_INCLUDED_REGION=36,94 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH490 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAATTTCAAATTATGCATACTATATGCAGACAACTTTGTAAACAAATAAAAAACAGGAAAAGATGGGTAAGTTATACATTAAAATGTTAACAATAATTATCCTTGAGTNNTNGGACCTTGTAATTTTTACGTTTATATATTTNCTTAATATTGAAAATTCATACTCTTGCAGTNNAAACTATGTTTTGAAGCCCTGTCACATAGATGAGAATTAGACTTATTTTCTATGACTCTGGAAGCAGAATAAG SEQUENCE_INCLUDED_REGION=36,250 P3_COMMENT=CL is ATAAAAAACAGGAAAAGATGGG P3_COMMENT=CR is TTCTCATCTATGTGACAGGGC P3_COMMENT=CM is ACCCATCTTTTCCTGTTTTTTAT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH504 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAACCTGGTTTGAAATTTGGGGGTCTGAGCCTCCACTGTNGTNTTACCTAGCAGGCTTCCCGGACTTAGTCTTACATGCACATGCACACCGTAAAAAAGAGACCAAAGAAAAATCTGCAAGANTTGCTCAAATAACACAAGACGGGGATGAACTGGATCTACAGGTCTAGAGTNNTTCAGAACTAAAGACGCCTGTCACACGGCTTAAGCAAGAGCAGGAAGGGGCTGCCAGGCCTCTGGGAAGACCTCCCTGGGGAGNGAGCAGGCAGACAAATGGCCCGGATAGAGATCCCCCGNTTACGTGATGTTTTCCTTCCTAAAGATAGCGC SEQUENCE_INCLUDED_REGION=36,331 P3_COMMENT=CL is AACCTGGTTTGAAATTTGGG P3_COMMENT=CR is TTAAGCCGTGTGACAGGC P3_COMMENT=CM is TTACCTAGCAGGCTTCCCG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH509 SEQUENCE_TEMPLATE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGCTCCTGGGAAGGAGGCTGAGACTCACATTGTNAGCTTTCACTCACTAAAGAGAGTCTTGCTTGAGGGGCTTGGACAAACAGGAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=35,86 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH512 SEQUENCE_TEMPLATE=CCTGCATGTGGCACCCACCTGAGGCCAGAGACATCTGGAACTCTAAGATTGGGCCCCACAGGAGGATGCTGTGTGGGTTCTGTGGATCCCAGCCACTCCAAAGAGGACACTCTCGGCAGAGGTTCT SEQUENCE_INCLUDED_REGION=0,126 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH516 SEQUENCE_TEMPLATE=TAGGGTCCTTCTCCCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTTNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG SEQUENCE_INCLUDED_REGION=0,17 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH519 SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCGTGGCACCCGGCAGCCTCCCAGGAGACAGCCAGGCAGGAGTGATGATCAGGATTGTGTTCAGTTTTCAGCATGTTTCCATGTACCCAAAAGTTAAGAGTCCCATCTACCTCTATAGTAAAACAAAATGATAGTAAATACATTCCATAAAAATGTTTTTCAAGCCATCAGTTTTGTTTCCGGCCTTATCTGTCAGTATTCCTTTCAGGCACATTCTGAGATTCACAACTGTGTTCTTTAAACAGCCAAAATTCCCTCTTCTCCAGCATGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAG SEQUENCE_INCLUDED_REGION=36,237 P3_COMMENT=CL is TTCAGTTTTCAGCATGTTTCC P3_COMMENT=CR is CTCAGAATGTGCCTGAAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH521 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTAAAAAGATATCCTCAAAGAATNTAATCTAGATCCTAGAATTTAGGATAATACTTAAAAATATGTATTTTAGATATACCTTCTCTACGTTATATTATTAAAATAATGTGTTTTCAATTTCCACATTGACAATAACTTTTCTGTAATAACTTGGTTTTTGAACCTATAACAAATACACATGAATATAGGGAGTTAATCATTTGGTAACCAATAAATGCTCCACAGACTTCCCATATTATAATAAACCTCAGGTAATATCTGATTCTTCAGAAATTCACCCA SEQUENCE_INCLUDED_REGION=33,284 P3_COMMENT=CL is AAATAATGTGTTTTCAATTTCCACA P3_COMMENT=CR is TGGGTGAATTTCTGAAGAATCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH522 SEQUENCE_TEMPLATE=AGAATTTTAGTATTTTCTTTACTTTCAAATTTTCTGTCTCTCTATATTCATGGAATTACTAATGATAATTATTTAATACTGTCATCAGCGATCTCTATTATTCAGATCATTTATGTACCTATTCCTAAGATGTGTTTTTATGTTTCTTTTTACTGTCCAGTCATATGATCTTTTCTATTGACATGTCTTGTAATTTTNAAATNATTTTCTAGATAATGTTGTATAAATCATTCAAAGGCTTGAGATTATGACATCATCTGTGAGAGAATATTTACA SEQUENCE_INCLUDED_REGION=0,276 P3_COMMENT=CL is ATTTTCTTTACTTTCAAATTTTCTG P3_COMMENT=CR is ACAGATGATGTCATAATCTCAAGC P3_COMMENT=CM is ATTTAATACTGTCATCAGCGATCTC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH523 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGTGTTTCTCGTCAGGGCAAGCCTTATTCACTGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=36,31 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH529 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCCTGATAATTTTGTTTTCTGAAAGTAAATGACAATGTCCTTAATTTTTCGACAAGGGGCTAACTGGATTTGTACCTAGAGGTAGTGAAATNATCTGTGTAGGCATAGTACTGGATTCTAAGGAATNAGTNAACAGGACTTGTGAAAAATCTCCCATTTCTTGCATCTTATATCTTTGATACTTCCATTTATCATGCTGGGGGTAGAGAGTATGAGAAAAGGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCG SEQUENCE_INCLUDED_REGION=36,188 P3_COMMENT=CL is TTCTGAAAGTAAATGACAATGTCC P3_COMMENT=CR is AAGATGCAAGAAATGGGAGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH533 SEQUENCE_TEMPLATE=CTTACATTGAAGAGCCAGTAGCGTTTGATAACAAGTTAGAGANNNNAAGTNAATGATGCCTCCAAAAGTTTGATCTGAGAAACACAAAGGATGAAAAGTNAGATGGGAAGAAAGTGGTAGGAACAGACATTTTTNCTTGGTTAGTTAATTGGCTGTTTTGTGTGTGTGTNTNTNTGGTGGGTGGAGAGTNTTGTATGTAGGGGTTGAATAGACCCTCACTTCAGGGCATATTAAGATCAAGATTTCTATGCATCCCATGCTAGATATATACCCAGAAGAAAGGAAGTCTATTGAAAAGATATCTGCACTCCCATGCTTAT SEQUENCE_INCLUDED_REGION=0,320 P3_COMMENT=CL is ACATTGAAGAGCCAGTAGCG P3_COMMENT=CR is GCATGGGATGCATAGAAATC P3_COMMENT=CM is AATGATGCCTCCAAAAGTTTG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH536 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCCTTTTGACAGGGCTTCCAAAAAAAGGCAGNGCGGCCCGGGTGTCTCCCTGGCAGCGGCGAGAAGTCAGCGCCGGGGCTGGGGACTTGTAGGACAGAGTCGNAGNTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTG SEQUENCE_INCLUDED_REGION=36,108 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH539 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTGCATCTCTTTTTCACCTTCTGCATACTGGTGCTGAGCCTAAGGCCTTGAATGTAAGTGCTTTTTAAGTATTTACCAAGTGGAAGTTTCCATTTTATTTTCCACCTTATATTATAGTTGATTGTGTCCTAGTATTCCTCCTAAATTAGTGGCTGAGGAGAGAGAGTGTAGCAGCGATCTACTCCTCTGCTGGGACACACAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC SEQUENCE_INCLUDED_REGION=36,168 P3_COMMENT=CL is TCCTGCATCTCTTTTTCACC P3_COMMENT=CR is CCACTAATTTAGGAGGAATACTAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH554 SEQUENCE_TEMPLATE=GCTCCACTGGGCCTAGGTATGCCATAGAGGCCACCCCTCTGAAGGGCATAGGTGGTAAATCTTGGTGGCATCCACAGGGTGCCAATGCTGCAGGCATCCAGAGTGTAGGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG SEQUENCE_INCLUDED_REGION=0,110 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH556 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAATCATTAATAAAATTAAGATGTTAAAACTGAATATCCATCTCACCAATATTTTTTGAGCACCTTCCATATTCCAAGCACTATTCAAGATAGTGGTGGTCAATGAACAGGAAACACAATGTTCTTGTACTTGAGGAGTTTATGTGATGGTTAGAGAGACAGACAATAAACAATAAACAAATAGCACTTCTCATCATGGTGGACCCTAGAGATAAGATATTTCAGGACTACAGCAAGAAGGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGC SEQUENCE_INCLUDED_REGION=36,205 P3_COMMENT=CL is GTTAAAACTGAATATCCATCTCACC P3_COMMENT=CR is CCACCATGATGAGAAGTGCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH560 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACGTTCTCCGTCCTATTTTAAGAACTCAGTTCTTCATAAGACTTGTGTGGTTTTCGATTTTTTCCCAAGTCTGGTTGATCCTTGTGTNGTNATTTTTTTAAATGTGTATCGTCTGTTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG SEQUENCE_INCLUDED_REGION=36,121 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH563 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGCGAGAAAGGAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTNAGACGGGCAACAGCTGATTGCCCTTCACCG SEQUENCE_INCLUDED_REGION=36,14 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH568 SEQUENCE_TEMPLATE=TCTGGGGATGCCTAGAACAAAAACAGACACCCTCGGCTTCTTCTCACCAGTCCACATGGGTGCCAAACAATCCCACATTCCTACATCCTCCCCACTGGGCTGCCTCCTTCACAACCTCACCAAACTTGGCTTACCGGGAAGCATAAAGCCAAAGCATTTAGTCTTTTATTGCAACATGGTCTGGCTGCAATACACATTAGATAATGACAGCCGNTGGTTGGAAAATGTCACCTNTNNNTTGNAANTT SEQUENCE_INCLUDED_REGION=0,247 P3_COMMENT=CL is AACAAAAACAGACACCCTCG P3_COMMENT=CR is GTATTGCAGCCAGACCATGT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH569 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAGAGATAAGACCTTGAAATAGTAGGATTCTTACAGGTGTATTACATGGTTTGAATTGAGATGATTACATGGGCATGTCTCACCTATAGTCAGGTAAACAAGTCTGGAAATCAAGAAATGGAAATGGTTTCTCTGTTGAACATAATAACCCCTTTGTAGGCTTTTTTAACTTTTTGTCCTGGTAACTATCTTGATTTGGTGCTCTTAGTTTCCAGTAACGGGAATTATTTCATTGGGGTTCCAAGAGGGTTGCATTAAACTTTATGAGACTGCCACTTGACCATTTGAGGATCCTTACGTTACTAAACAAACAGGCAAAAAGGGACTATTCTACTGGCTA SEQUENCE_INCLUDED_REGION=36,342 P3_COMMENT=CL is CATGGGCATGTCTCACCTAT P3_COMMENT=CR is TTTGCCTGTTTGTTTAGTAACG P3_COMMENT=CM is GTCAGGTAAACAAGTCTGGAAATC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH570 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGAGATAACGTTGGGAGTNGTAAAAACTCAGTTTATAATCACATTGAATGACATACACTTTAAGGCCCTGAAGTATGTTTCTGTTTTGTCTCTTTTTAAATTCTTATACAGTGGGTATGTGACAGGAATAGAATCAAATGAAGAGGAAATTACAGGGCAGCAACTTTGGACAATAGTGAAAAGGAAAGATGCATTGCTTGCTTTAGTGTTCATGTGACCACTTTATCCTAGAAGCAGAAATTAAGTGGCAGAGAGTCACCTGTGAGTGTGACTAATGCCGTCAATTCCAGGAGAACAAGAAAAAAAAAGCCTTCTGTCCCAACT SEQUENCE_INCLUDED_REGION=36,326 P3_COMMENT=CL is GGCCCTGAAGTATGTTTCTG P3_COMMENT=CR is GTTGGGACAGAAGGCTTTTT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH572 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAAGGCTGAATCTTAACAGCCTTTTGATGCCAAAGCCACTTTCAGTCTTAATTCTTTTTGGAGCCTAAGATCAGTGCAACCCTCCAAGGCTCCCCAGTATCTGGCACATCTTTCCCTTTTCATCTCCGTTTGTGTGTTTGGCCAAATAATATCTCCCCCAGGGACGTCCTCTTTCTAATCCCTGAAACCTGAGAAAATGTTATCTTATGCAGTGCTATGGTTTGAATGTGTCCCCCACAAAGCACACATTAGAAACTTAATCCCCAGTGCAACAGTNTTGGGAGGTGGGGCCTAATGGAAGATGTTTAGGTCATAAAAGGTACCGAGCTCGAATTCGTAATC SEQUENCE_INCLUDED_REGION=36,283 P3_COMMENT=CL is AATCTTAACAGCCTTTTGATGC P3_COMMENT=CR is TGTTGCACTGGGGATTAAGT P3_COMMENT=CM is AAAGCCACTTTCAGTCTTAATTCT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH574 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAATTTAACCATTATTAGATTTTCTCCACTCAAGGCATCTCCTCATAAGCAGTNTTTCCTGACCATAAATCTTAGTTGTCCTATCCTCGGACACTTGTTATTTCATCACCCTGCCTTTGCAATTATCTGAAAATATCTTTGGTTATACAATTTATCGTGTCTCCCCACCCAACCCCCATCTCCTAGAACCCACTTCCCTAACCCGAAAATACGGGGCAAGAAACCTGTCTGTACTGGTCACAACCCTATCCCTGGGCCTACAACCGTGTCTGAGTCATAGTAATCCCTCAACAAATTTTGTGTGAACCGAAAGAAAAAAACACTTAAGACAATCTAAG SEQUENCE_INCLUDED_REGION=36,340 P3_COMMENT=CL is TTCTCCACTCAAGGCATCTC P3_COMMENT=CR is ACTCAGACACGGTTGTAGGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH576 SEQUENCE_TEMPLATE=GCTATATAGACTACAATATGCCTCTTAAATTTATCCCAATTCACTTAAAGTTTATCCTTCCCTGTACTGCAGCCTAAAAACTCCAGGCAGTAATCTGGACAAACGTAGGGCTCAATTCTTTGTTTCTATCTTTTAGGCATCATTGTCCCAGTCTAAATGATGTCTATATTTTCTTTGCATCTTAGTTATTTCAGGTGAGAGATTAAATCATGTCTTTGTTATTCCATCTTGGTTGGAATTGGAAGTCCTAGGTTANGTGTAAATTTAAGAAATGAAAATGATATGCGTGGGCAATTAAGTTGTCAG SEQUENCE_INCLUDED_REGION=0,306 P3_COMMENT=CL is CAATTCACTTAAAGTTTATCCTTCC P3_COMMENT=CR is CTTAATTGCCCACGCATATC P3_COMMENT=CM is TGTACTGCAGCCTAAAAACTCC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH578 SEQUENCE_TEMPLATE=CCNNNNGTTCATGCTCAGGTCGACTCTAGAGGATCCCTGTGGGTTTCATCAGACCTGAGATTCAGCATGAAATCTACCAAAGGTACCACAAATGTAACCTTGTCCAAAACGAATCTCAGTTTCTGCATATGTAAAATGGGAATGATAAGAGCACCCACCTACCTCATGCAACTGTTGAGAGAAATAAATGAGACATTGTAAGTAAAGTTTGTAATGCACTGTTATGGCCTGAATTGTGTACCCTAAAATTCATATGTTGAAGCCCTAACACCCAATATGNCTGTATTTGTACATAAGGCCTTTAGGGNGGTAATTAAGGTTAAATG SEQUENCE_INCLUDED_REGION=37,289 P3_COMMENT=CL is TCATCAGACCTGAGATTCAGC P3_COMMENT=CR is TAAAGGCCTTATGTACAAATACAG P3_COMMENT=CM is TGAAATCTACCAAAGGTACCACA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH584 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGAATTGACCCACAGCATCCCCACAGAATGTGATTTCTTGAGTCCTTTGTGAAGGTCACCTCCTCTAACCTCTCTATATATACATACTCAAACTTCTTTGGAGACTGGAAGATTGGAGGTACTATCTTTTAGAGTAGTTTTGCTGAAAAGTGATAAACTCCTTTCACAGTGTGGGAAAAGAGATCCAGTAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCT SEQUENCE_INCLUDED_REGION=34,161 P3_COMMENT=CL is TCTGAATTGACCCACAGCAT P3_COMMENT=CR is ACTTTTCAGCAAAACTACTCTAAAA P3_COMMENT=CM is CCCACAGAATGTGATTTCTTG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH585 SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCTGCACCAGATCTTTACCCTCTGGGGGGCTGAATCAACGAGTNCTAGACTGAAGCACCCAGGCACAGCCAAGGGTGAGGGAGCCATGATCCTGAACATAGCATTGACTGATTAATGAGACCCCCTGGGCCTGTCTTCAGTGCACTCCCAGTATGGGGACAGCCAGACTGGCGCACCCCCTCCGTGAGGCAGGAGACCCTAGGAGCGCTCTCTGGGGACAGTGACTGCAACTCTCGGTTCCCTTACAAATGGCTGAGTTGTCACCAGAAAATGACACTCACATATGGCAAACCCTGTCCT SEQUENCE_INCLUDED_REGION=34,298 P3_COMMENT=CL is TGCACCAGATCTTTACCCTC P3_COMMENT=CR is CTCAGCCATTTGTAAGGGAA P3_COMMENT=CM is CTAGACTGAAGCACCCAGGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH590 SEQUENCE_TEMPLATE=GTTCATGCTGAGGTCGACTCTAGAGGATCCCCCTACCTTCATATACTATACTGTCTCTGTTGAAGGAGGAAATAGTGCAAAATAAACTACTTTGATATGCTGGATCTTGTTAATGAGTNATTCATCTAGTNAGCAAATCATTACTGTTCACCTTATGTGTGCCAGATATTACATCAACACTAGGGATCCTGTAGGTNNGGAGGGAAGTCCCTGCCTTTGTGAAACTTCAAATCAAATGTGGGAGACAGACACTAATCCAAAGAATTTCACCAGTAAG SEQUENCE_INCLUDED_REGION=32,245 P3_COMMENT=CL is ACTATACTGTCTCTGTTGAAGGAGG P3_COMMENT=CR is TGTCTGTCTCCCACATTTGA P3_COMMENT=CM is AACTACTTTGATATGCTGGATCTTG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH592 SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGCAGTNNCCCCCACAGCAGGGTNCCACCTTTCAGAGTNNAAACAGGTCCTCACTGAACAGTCAATGTCACTTCCAGTCGGGGCACACTGTGTCCNAAGTTTACAATTNNAAACTCTGCTGTCTTGAGAAGNACTGTGGTTAATTCCTGGTACAAACCTTGCATCAGGAGAGTCCTATGCCATCTTCCACTAAAGCAATCAAGTGTGAACANCAGTNCAGAGGGGCTGGTCTGGCCTTAACAATCCTCAGATGGTGCCAGAGTCGGTAGATGCCAGGGGTAC SEQUENCE_INCLUDED_REGION=33,221 P3_COMMENT=CL is AGTCAATGTCACTTCCAGTCG P3_COMMENT=CR is GTTCACACTTGATTGCTTTAGTG P3_COMMENT=CM is CATTGACTGTTCAGTGAGGACC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH599 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTAGAGGNNNCTTATTTTTNCTTTTGTTAAAAAATCAATTTATTACTTTTAAAGTAGTTTTTGTAGATAAAGTTGCTAGAATATGTTGCTAAGCAGGTATGCTTTAAGANCTAGATAAGAAAAAAAATTTCTCATTATTAGGCAACAATTTGTATTGGTCGATTTGCAGCAACCAGAGAAGGAANCCAAACCACAATCCCTGCAGCAACCAGTCTGGGAAGTCAANCCACAATCTCTGCAATCATCAGCCCAGAATAATCAGGAAATGGCCAATGGCTGCCATATTTGTATTTGTTATGTAATTGTTGG SEQUENCE_INCLUDED_REGION=36,312 P3_COMMENT=CL is AGTTGCTAGAATATGTTGCTAAGC P3_COMMENT=CR is GGCTGATGATTGCAGAGATT P3_COMMENT=CM is AAAAATTTCTCATTATTAGGCAACA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH604 SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTGTNNATCGCTTCCTCAGAGANNCCGTCCCTGACTCCCAGACAAGGNCGGGCTGGGCCTTTTGTATGTNNTCAGAGCACCCTTGGTGATTCCTTAAGCACTTAGCACAATTTGTAATCATACACCTATTTCTGCAATTATTTGTTGTAGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGA SEQUENCE_INCLUDED_REGION=36,118 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH618 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAAGCGTAAGAATACGTGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT SEQUENCE_INCLUDED_REGION=35,21 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH623 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCTTCATAGTNNGCGTNNTACAAAACAACTTAGTNTTCACATTCAATTCAGTAATTAACATGGTGTTTTNTTCCAGTGAACAGAAAAGTTATTATAAAACAATATATTTCCATTCGTCATTGTCTTTTCTCACTTCTGACTAGCCACAAGGTCTTGACAACTTTTTTTCTATGTAAAATGAGACTGTGCTATGAATATCTCAGAGG SEQUENCE_INCLUDED_REGION=34,206 P3_COMMENT=CL is ACATTCAATTCAGTAATTAACATGG P3_COMMENT=CR is TTCATAGCACAGTCTCATTTTACA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH629 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTGCCCACAGCAGATGGTGACAGGGTCTACCAGGTAGTTCAAACAGATGACGCAGGTGAGTTCCTTCTGGAAGGCATGTGAGAAGTCTGAGTCCATTTTCCTAAGGAAAGAAANCCACAGGAATTTAATCTTCTACCCTGGAGAGACAAAGATCCAAGCAAAGTTTGAATCAGATCGTGATCGAATAATATCCTTTCTTTCTAAAGAAGTATAGGCTTTAATCTGCA SEQUENCE_INCLUDED_REGION=33,228 P3_COMMENT=CL is AACAGATGACGCAGGTGAGT P3_COMMENT=CR is AAGAAAGGATATTATTCGATCACG P3_COMMENT=CM is CCTTCTGGAAGGCATGTGAG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH633 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCCAAAGAAAAGTGACTAAATCAAGAAGGTTAACTCACATCCCTGCCTGGGAACCGNGANGTCCTGGCCTATAGTAACAAAAATTTCTCTTTCATGCAATGTCTACTCTATTGCAGGCACAGGGCTAGGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATG SEQUENCE_INCLUDED_REGION=33,132 P3_COMMENT=CL is CTTCCAAAGAAAAGTGACTAAATC P3_COMMENT=CR is CTAGCCCTGTGCCTGCAATA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH636 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTATCCCCCATCCCAGCCCACAACCATCTCACTGATGAGTCTGTAACATGAAACTAAATCAAAAAGATCCCCAACAGAATTAGAAAAAATATTAAACTCACTGCTTGCTTGTTGATTTAATCAACCTAGCCGGCTGTCATGTGGGATTAGAATAAAATAAACACAAAAATGAAAACACACGATTGCTAACAAAGCAGATTCTTTTTTCAAGGCACACGTAAAGATAATAACTTCAAAGTTGATTATATTAACTTGTCTTCCCATGATAACACG SEQUENCE_INCLUDED_REGION=36,276 P3_COMMENT=CL is ACTCACTGCTTGCTTGTTGA P3_COMMENT=CR is TTGAAGTTATTATCTTTACGTGTGC P3_COMMENT=CM is TTAATCAACCTAGCCGGCTG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH640 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTATAATCCACACACCTTGAATTAAATAGCACGTGAGAGGGNTCCTGGGGTAAATGAGCATTTAATCACTTTTCATACCCTTTTTATTGTCAAGGGAATGAACAGGGTCTGGAAAAGTGGTGAGGTATTTTGTTTTACTGCAGTTGCTATTTAGATTCATGGAAGTTTGAATAATGACTATTACTGAAAAAGATTGCTTTGGCCAGTTTAGATATTAAGAAAAAACCTGCAGAAAGATTATGG SEQUENCE_INCLUDED_REGION=35,242 P3_COMMENT=CL is AATTAAATAGCACGTGAGAGGG P3_COMMENT=CR is TTTTTTCTTAATATCTAAACTGGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH642 SEQUENCE_TEMPLATE=AACCTAAATTTTGCAAAGCAGTCTGGTCATATCGGAAAATATGCCTTTCTAGCAAAGCCTTGGTAAAATCAGAAGTGTTTCAAATTATGTCCTATTACTAAAGAGAACAAATTNTTACCCAACTTATGCAAATAACTGTATTGCCATGAGTTAAGAATACCCACAAATAGTTTCTCAATTTTGGAGGAATGAGGCAGAGAGAAATAGAAATTCTTCAGATTTGGCTAACAGAAGTATACTTTATCCAATGTTTTGTTAG SEQUENCE_INCLUDED_REGION=0,259 P3_COMMENT=CL is TGCAAAGCAGTCTGGTCATA P3_COMMENT=CR is CTGAAGAATTTCTATTTCTCTCTGC P3_COMMENT=CM is CGGAAAATATGCCTTTCTAGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH644 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTTGGTNAAACACTGTCTAGATATTGTTGTGAAGGCATTTTGTAGATGTAATTAACATGTTCAATTAGTTGACTTGAAGAAAAGGAGATAGTCTTTGTGGGTGAACCTCATCCAATCAGTNTAAGGTCTTCAGAGANAAAACTGCAGTTTCTCTGAGAAGAAGGACTTCTATCTTACGACTATAATATGGAAGAACTGCTTGAATTTCCATCTTGATGACCTGTCCTATGGATTTCAGACTTGTTAACCCCTGCAATCATCTGACCCAATGATACAGGAGTTAAAAAGAAATTATTTAGGCAGAGAGT SEQUENCE_INCLUDED_REGION=35,311 P3_COMMENT=CL is AAACACTGTCTAGATATTGTTGTGA P3_COMMENT=CR is CTCTCTGCCTAAATAATTTCTTTT P3_COMMENT=CM is AACATGTTCAATTAGTTGACTTGAA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH646 SEQUENCE_TEMPLATE=GCTNGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTGGAAGGAAGGACTGTGTTGTGTTCTCCGTTGCGTCCCCACCACGTAACACAGCAGCACTTAGCAGGTGCTTGGTCTGTATTTATTNAAAAATGNATNNATGNATATGTTGAATTAAATGNAAGTNAATTAAGTGAAAAGGATGAAACAGATGGAAGCTTCATGTTTCTTCCAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTNTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT SEQUENCE_INCLUDED_REGION=36,179 P3_COMMENT=CL is CTGTGTTGTGTTCTCCGTTG P3_COMMENT=CR is TGAAGCTTCCATCTGTTTCA P3_COMMENT=CM is GTCCCCACCACGTAACACAG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH647 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTTGCCATATTATTAAAAGATATCCATCCTACTACTTTTTTACTATTATTTCACTTAACCAGATTGGAAAGAAATTTTATTTGATATCCGCAGCTCTAACCTAGCAATGGAATTTGAGTAATATGTTCCCTACAGCCATATTTTAATATGTAAAAAAGCTTATATCCCTGAAGTTCAATATTATGACACTATACACTATGTTATGACATATCTATTTTCTTAGAATGAAGTGANGAAAGGGAAGACTATGGTATTCTCAGAACTTGGGACTCAAACTCATATTATTGCAGGG SEQUENCE_INCLUDED_REGION=36,295 P3_COMMENT=CL is TGATATCCGCAGCTCTAACC P3_COMMENT=CR is CTGCAATAATATGAGTTTGAGTCC P3_COMMENT=CM is AGCAATGGAATTTGAGTAATATGTT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH648 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCCAGTNATTTCAAGTGATTGCTGTGGGCATTCTATTTTAGGTNCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGAGAAACATTAGAGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAGGTAGGAAACATTATTCCCCTTTTTTAATTGCTTTGTTATTTGCTCGTTTAGTTGGATTTTGTGTACTTATTTGCAAATACATATTAGTTAGTGGCTTTTTGTCTTTTAAGAGAAGTTCTGCTCTACTTATGTAGGCAGTTTTCAACTCAGTTATCTCTTGAATTGAAAATAGGAGTAAAGGAAATTAAAATTTTCT SEQUENCE_INCLUDED_REGION=36,340 P3_COMMENT=CL is TTGCTGTGGGCATTCTATTT P3_COMMENT=CR is TCCTTTACTCCTATTTTCAATTCA P3_COMMENT=CM is CTGGTACTCAGGAATCATGGTG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH650 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAACAGACCTGTGCCAAGGTTCACCTAACCTAGTACCTAACCTCATGTTTGTTTTACATGCATAAGACAATTCTGGGAAGATTAAAGTGTAAATTCCTAGTGGAGAATTTGAGTGGCCATTTTAATTATTTAATTAATTAATTTTGGTCCAGCACTCTTGTCTGATATTTTCTTCAGGTGACAGACTACACTTTTGAGAAACAATGGTGTTTTTCTTGTGCANCTCTGACTTGAATATACCAATCTGAACCGCTGCCAGTGGAGAATCTAACCACCTTCAAAATGATATCACTTTCATTCAATAATAAACAT SEQUENCE_INCLUDED_REGION=36,314 P3_COMMENT=CL is CTCAACAGACCTGTGCCAAG P3_COMMENT=CR is ATGAAAGTGATATCATTTTGAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH652 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGGTNACAAGTNGAGGAAAGCAGGAAGGGAGTGAGAAGCCTGAAGCCTCTAGCCACCAGTTTCCCCAGGCAGTCCCCCATCCAAGCAACAGTCAGGCCTGACCTTGCTTAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG SEQUENCE_INCLUDED_REGION=36,111 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH657 SEQUENCE_TEMPLATE=ANGNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAACATGACCACCCTCCCCACCATTGCTGCTGGGATGAGCACCCAGACCAGGGCACCAGAAAGCCACGGAAGCCACAGCCACTAGCCCTGAACCTTGCACACCCCTGGAGTTTCTCTCCCCTCCCTATCCCCTCACCAACACCTTCCAGTGCTTATTCTGCTGTGTCAAAATGATCCTTCTGTTGCTGCACTGTCATTACTGTTGTATGGATTTATAATTATTGTCCAAAAAAGCCCCGAGCCTGGTACAGAAAAGGCATGGGGAAAGATGTGTCAGAATGCACAGTG SEQUENCE_INCLUDED_REGION=38,290 P3_COMMENT=CL is GACCAGGGCACCAGAAAG P3_COMMENT=CR is TCTGACACATCTTTCCCCAT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH660 SEQUENCE_TEMPLATE=GCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCCTGGCCGCCCACCCCGCCCCGCTCCCTGCGAGGAGCGNAGNATCTCAGAAACCAGAAACGGCGCCCACCGCACCCCACACGGCCCCACCTGGGGCCCNTCCACGTGNATGTCCTCGCGGAACTGGGCCAGCGGGTAGACAAGTCGCAGAAGAGCGAGGAGTCCGCGGCGGGGAAGGCGGCGTCCCGGAACAG SEQUENCE_INCLUDED_REGION=32,197 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH661 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCCTCCCGGTGCCGCCCGCGCCTGCTCCTAGGTTCTTTTTAAGAGGTGTTTTAAAGGTGTTTCACTTTATACTAGAATAACACAAAGAAAATATTAAGAAGATTTAACAAGAATAAAATCATAATTAATTACAGGCATATCAGTGTCTGAGAAGATGATGTGTAGAAAACTGATTTGAGCAGGAATAGTCTGTATTACAGTTTCCTGATATGATTTTCTCGGTAACTGGGAGAACTAGACAGAAGATAAAGGTAATAGGGTTTA SEQUENCE_INCLUDED_REGION=36,265 P3_COMMENT=CL is GCCTGCTCCTAGGTTCTTTT P3_COMMENT=CR is CCCAGTTACCGAGAAAATCA P3_COMMENT=CM is AAGAGGTGTTTTAAAGGTGTTTCA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH665 SEQUENCE_TEMPLATE=GGAACACTATGTAGAGANACACAGGTGCAATTCCTNCTNCCCTTTGACCTTATAGTGAGTGNCCCTGCATGAGGGTCATGATAATCACACCAAAAATACCTAATTTCAACTTAAGTGAGAACTGTGAAAGCCTGACCCTAACTAGGAGGCCTGAAGTGGAATTTCTGAGAAG SEQUENCE_INCLUDED_REGION=0,172 P3_COMMENT=CL is CCCTTTGACCTTATAGTGAGTG P3_COMMENT=CR is TCTCAGAAATTCCACTTCAGG P3_COMMENT=CM is CCCTGCATGAGGGTCATG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH670 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGTTACGATTCTTTGCACTAAAACATTTTTATGTATCTCATACTTAGGTATTGGCATGTTTATGTTTATAAAACTTCTGATAAATGTTATACATGTCATGAAATTTAAGGGTGAAATATACATACATCTGAAAGTAGTGCCAAAAAACTGAAAATATATTTGCACACTTAAATGTCTTTGTTTCTAGCGATATTCATCTTCAACCATAATAGCCTTTTCTGAAGTGGTTTGTTTGGTTTTTATATTAGGTTTTCGTTGTCAGTGTTCCTTTCTAATTG SEQUENCE_INCLUDED_REGION=36,281 P3_COMMENT=CL is ATCTCATACTTAGGTATTGGCATG P3_COMMENT=CR is ATTAGAAAGGAACACTGACAACG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH672 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTACCTTGCCAAATGTTCCATTATAAAAGTTATAGTATCAATGATAGAAACTTTAGGAAATTTAAGATGGCGGACATCCTTACAATTTTCTTTGTAAGTACATTTAAAAAAGATGTCTGTGTACTTCAATTGGAAGGGAATGAGGGTTGACTCAAAGCATATAATTAATTATCCTTGGCTATTTTACACTGTGGCTAATTCAGNTGTTACTCTCTCAGGAAGACAGGGAGAAACTTGATTTATTTTGTCTGGAATCCTATTCTTATTAAAAA SEQUENCE_INCLUDED_REGION=36,273 P3_COMMENT=CL is TACCTTGCCAAATGTTCCAT P3_COMMENT=CR is TTCTCCCTGTCTTCCTGAGA P3_COMMENT=CM is GAAACTTTAGGAAATTTAAGATGGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH679 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCACAGTCTCCTTTCCTTCTGCTCAGTACATTGAAGACGTAGTTTCTTTCATTTTGAGGACTTCTCTTAAGCAAAATATTTGAGGACTAACTAATAAATATTTAGGAAAAACTTGTCTCAGTACAAGTGGACATTTTTGTNATTTCTTTGGTCCTCACTCCAACCATCATTTCCCCCTTCTATGCCATTCATCTTTAACAAAATAAGTTTTCCTGTTGCCAGGGAGGTAAAACTTTCACTCTACCCTCTTAGGTTCAGTATTCGGGGGATTGCAAATTAAACCAACAAAACACAGCTCCACCCCATCAACATCTGGGG SEQUENCE_INCLUDED_REGION=36,319 P3_COMMENT=CL is TGCACAGTCTCCTTTCCTTC P3_COMMENT=CR is GGAGCTGTGTTTTGTTGGTT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH683 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTTCAATGAAACAAGACATTAGGAAAGAGAATGCAGAAAAAGTGTTACTTTGTCTACTTTAAATTTTCAAGTTGTACATTATCTATTCTCGTTTATCTCTGGGTNAATGACGATACCTTTGTCAGGGTGCGGACAGTAGGATAATAAGTAAGTTGTAAAGCCCAGTACACTTCTCTTGGCACTGAAGCAAATCAATCCCCTGAAATACACTGAAGACAATGGATGATCATTAAGATTTTCAGACAGGGTTTAAACGCATTAAAATGTGAAAA SEQUENCE_INCLUDED_REGION=35,275 P3_COMMENT=CL is CAAGACATTAGGAAAGAGAATGC P3_COMMENT=CR is GCGTTTAAACCCTGTCTGAA P3_COMMENT=CM is TTATCTATTCTCGTTTATCTCTGGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH684 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAGGTGGACCAGTAGCGTTCTGTGGCCTGGGGGCGGAGGCCTCTAAGACACGGGTTGTGGCTATAAGATGAATGACAGCCATAAGGCCCACTCTGACATCTCTCGGGGCCGATGCACTATGTATCACTTAGCAAGATGCTGTAACCTGGTCTGGGGATTTCCAACCCTGGTCTGGGGAATTACTGGGTCTCCATGACAACAGGACCTAAAAAACCTGGTTGCCCTAGAGACAAGGCCTCCTCAGCACATATGCAACCTTCTTGAACCTTAAAACCAACCTTACTCTTACA SEQUENCE_INCLUDED_REGION=35,292 P3_COMMENT=CL is GTGGACCAGTAGCGTTCTGT P3_COMMENT=CR is AGGTCCTGTTGTCATGGAGA P3_COMMENT=CM is GGGCGGAGGCCTCTAAGA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH688 SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAAAGATTTTTAAAGTGTTTGTTTGGAATGTGTCAAGAGTNNTTTTGATGTGATATATCTAAGGTNACATGCAGAATCACTGAAATATTAAATGCCAAATGGTTTGCAAATAAACATGCAGATAATTATTTTNCTTCCCTAAATTTCAAGTTGGCACTAGCCAGCACTCTTGCCAAATGTTCCATCTATTTTCTTCCATTCTCAGCATCTTGCATGCTACCGTCCACGCTTCTCATAGTGATGCCTCATTACTGATAAGTTGTAATATCACTTGGTTGCCTATGACATTGAATCCAAG SEQUENCE_INCLUDED_REGION=35,300 P3_COMMENT=CL is TTAAATGCCAAATGGTTTGC P3_COMMENT=CR is TGTCATAGGCAACCAAGTGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH691 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCCATGCCCCACAAAAACATACTTTTTGTGCTCCTGTTTATTAACTGGTACTTACTAAAAGCCAAATACTCCTAGAACTTTGTAGTATTATCCCTTTTAATTCCCACATCAACTAAGGTAGATGCTGTCTGTTATTGACTAAATTGTGTCTTCCCACAAAATTCAAATGTTGTAGCCCTAACTCTCAATGTGATGGTATTTAGAGACGGGGCTTTTGGGAGGTCATTAGGATTACATGAGGTCGTGGG SEQUENCE_INCLUDED_REGION=34,118 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH692 SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCAAAAGAGGNNTAAAGAAACTAAAAGCAACAAAACCAAACTTAAAAAAAAAAAGGCAAAGGTTCTGAACTGCAAAGGAAATTGTCATACCATTAACTCTGCTGAATGAAAGAGGAATACAGCCTCCTCCGGTTCTTACAGTTGTGTATAAAATAAGGTAGCAGATTAGAAATAGAGAAGATATGCTAAAGATGAGATGTGACTGATGACAGCAGACAGCAAAGGAAGGAAGTATAATTGCTAACATGAAATAAAAGTGCTACAG SEQUENCE_INCLUDED_REGION=36,267 P3_COMMENT=CL is GGTTCTGAACTGCAAAGGAA P3_COMMENT=CR is TGTAGCACTTTTATTTCATGTTAGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH693 SEQUENCE_TEMPLATE=GTGTGGGGGCTGGGATGAGAGGTCTACTTGTGGTACTGGAGGTTTCACTGGCTTGTGCTAGAACTAGNAAAGNAGAAAGAGACAGNGATTGGCTAACGCATGGCAGTAGTGGGCCCCAAGGCCTGAGTAATAAGAAAAAATCATTAGATAAATGTCTCATGACCAAAACAAAGTTCAAACANTAGGTGCAGCACANNNGGGTTTTCTCTGGTCATAGAATCTCTTAAAAGGGAATCATGACAGATTTTCTTGGCTTTAAGTCCTTTATTTAGTTCCCTCTACCAAGTGTATCTTTTAAAGCCTTGTTAAGA SEQUENCE_INCLUDED_REGION=0,311 P3_COMMENT=CL is GGCTGGGATGAGAGGTCTAC P3_COMMENT=CR is TAACAAGGCTTTAAAAGATACACTT P3_COMMENT=CM is TGTGGTACTGGAGGTTTCACT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH694 SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAAAATGTTTATGTTGATATAACTCAAGCCCAGGCACCATTTCTCCTCATTTTCCTTTCAATGTGCTTTTCAAAAATTATGGCATATGTCTGCCTGAAAGTCGGAAACATTTTTGACAATAACTTGGAACTTTCTCTAACCTAATACACTAGATTTAAAGTGAATTATACCGCCATAGGTTGACATGGAGCCATTTTAATAGCAGGTAGTTGACCTGAGAACTCCAAAGCAATTGCTTACATATTGAATTATCTTATA SEQUENCE_INCLUDED_REGION=36,215 P3_COMMENT=CL is TGTTTATGTTGATATAACTCAAGCC P3_COMMENT=CR is ACCTGCTATTAAAATGGCTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH698 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGATGGCAGACACCTGAGGAGCCAGGTGCCTGAAATTTCCTCCACCTGCTTGAGGAGCGCCAAAGCCCAGGTGCTGGCTAGACAACCCCTTCTGGCTGCCTAAGCAGGTGGCAGAGGAAGGAAAAAAGGTCAGAGGCAGAGTNTTGAACCCTGCCTCCCAGGTGGGTGGAAGATGCCTGTCGCCAAACTAGGGCTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT SEQUENCE_INCLUDED_REGION=36,198 P3_COMMENT=CL is GGCAGACACCTGAGGAGC P3_COMMENT=CR is CAGGCATCTTCCACCCAC P3_COMMENT=CM is AGGTGCCTGAAATTTCCTCC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH701 SEQUENCE_TEMPLATE=NTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGTTTCATCATATGCGAAACATGGATAAAAACAACTACCTTGCCTTACCTACTTTACAGAGTNTCTCCCCACAATTCCAAGAATTAAATGAAGAAAATGTCTGTAAAAGTGTTTTGCATGCTATAATGTAAAGCATCATTGTGAGATCTGTGATGGTTATCCCAGGTCTTTATTCCAACATCCATTTTCTTATTAATTTTTATAACTTCCATTTTTATCAGTGGGCCTCTTATTCACTAGATTTATCTGATGAAAGCCATCTGGAATGCATATTTACCAGGTTA SEQUENCE_INCLUDED_REGION=35,156 P3_COMMENT=CL is TCATCATATGCGAAACATGG P3_COMMENT=CR is TCACAGATCTCACAATGATGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH704 SEQUENCE_TEMPLATE=TCGACTCTAGAGGATCCCCCTAACTCTTGTNTTTCTAAGCACTTAACAATGGATAATGNAAACATTCTAGGGAAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTTGCATTAATGAATCGGCCAACGCGCGGGGAG SEQUENCE_INCLUDED_REGION=19,55 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH707 SEQUENCE_TEMPLATE=TATATGGATTTTTATCTTGGGTNTAATTATTAGGTTATTACAAAAGTAATTATGTTTTTTGGCATTATTTTCAAATGGCAAAAACCGCAGTTACTTTTGCAACAGCCTAATATTTAATAGCCTTGCTTCTTTCTTGGAAACTGTTCTTGTTACTTTGAGTNTCTACCAGTNCCATCTTTTCTTGTTCACGATGTAATGTCTGTTTGGACAGATGTATCAAAGTACCTTAATCTAAG SEQUENCE_INCLUDED_REGION=0,236 P3_COMMENT=CL is TTTGGCATTATTTTCAAATGG P3_COMMENT=CR is TGTCCAAACAGACATTACATCG P3_COMMENT=CM is AAAAACCGCAGTTACTTTTGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH714 SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTAACCTGGACAAATGACTATTGATAAAGAATTTATTTTCTGTCACCTTTAAGGAGAGCAGAGGGTGGTACAGCCTTTGTATTATTTTTNCTTTTTTAAAAAAAGTTTGTTTGAAGCCTCCTTCATGAAAACTTCAGCTGTCAAATCTTGGCAAAAAATGTGTGTTCATCTATATGATAGTTCTTAGGAGAATCAATAG SEQUENCE_INCLUDED_REGION=35,201 P3_COMMENT=CL is TTCTGTCACCTTTAAGGAGAGC P3_COMMENT=CR is TGAACACACATTTTTTGCCA P3_COMMENT=CM is GAGGGTGGTACAGCCTTTGT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH715 SEQUENCE_TEMPLATE=AGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTCTTCCTGAGAACAAAGGGCTCAGGCTATGATGTGGGGAGGAGGCCTGGACCTGTGTCCTATGCCTGGGNCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT SEQUENCE_INCLUDED_REGION=37,76 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH718 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTCTTTGGCTATCTCATTCTCACACCTCCCTCATCACCTTTTTCTTGGGCACGTGCATTTCTTTTTCTCCTAGGGTTGAGCACAGATCTAACTTGAATAATTAAAGGAAGCTTTGTGTAATGAAAGCCAAAGCAGAAAGGAACAAAAGACTTTTAGCATGTAATAGAAGGGAATAGTGAACATGGAGGCAGAGGGAGTCATTTTTGGGGTGTAAGAGTCGATTATCTTTCTTGTCCCCTGGGCCGTTCGTTAGGGTGGCTTTACTTTGTTAATCCTGCACTT SEQUENCE_INCLUDED_REGION=36,209 P3_COMMENT=CL is TGGCTATCTCATTCTCACACC P3_COMMENT=CR is AAAAATGACTCCCTCTGCCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH719 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAAGTNTCTTAAATCTTTGCTTCAAAGAAGTTCTTGCTCAGGAAGTTATTCATTCAGCAACCTAAAATTGTTTTTTGAGTACATATCAAGCACAGGGTTCTGAGCAATGTCTTAGGAAGACCATAAAGGTGAATAAATGAGTGTTTCTACCCTGAGGAATTTATCAAAGATGTTAAGTTATCTCCTTAGAGGTATAAGTCATATAGGCATATTCTATGTATACTAAAGGTGGTATGGCATAAGAGTACATATAGTCACCAACTTANGAATTGTTTGACTTGGNCGGGCGCAGTGGCTCACG SEQUENCE_INCLUDED_REGION=36,303 P3_COMMENT=CL is TTGCTTCAAAGAAGTTCTTGC P3_COMMENT=CR is TATGTACTCTTATGCCATACCACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH720 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCCAAAGAGCNTGCTTTTTAATTAGTNCAACATGCAGCAGCAAATNAGGGAAATGGGGAAGAAATTATTTGTCATCAAATTGAAATACATGTAAAGAAAACACAAGCAAAAATGTATTAAAGCCTTTTATGAGGAGGCTTTTAGTCAACATTTGGCTTGGTTAAAACCTTGAGCCAAATGTTTATTAAGGCACAGCAGTAAAGGAAGCAAAANCCATTTTTTGTACCTTT SEQUENCE_INCLUDED_REGION=36,166 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH721 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATTGGGTNTCTCTCTCCCTACTGTGTAGAAGACCCTTACTTAATTGCTACTTCCTTAATGATGCCTTTCCCAGGACAAAACTCCATGGTGACTTTGTGTTGCTCTGATAGCCCTTCTCATGGGTGGTAACTCTGTCATTTGTTTTCCATCTGTCCATCCATTCCACAGACAGTGATTACAACCAGGATTGCTGCTGGGCTGTGAAAATACTAAAAACAATGAGGCAATGTGTCTACCTTCAAGAGGTTTACAGTCTATAATCTGTGCTATACTGTAATGATTTCTGTGACTGCGTTTGATATTTCTAG SEQUENCE_INCLUDED_REGION=36,311 P3_COMMENT=CL is AGGACAAAACTCCATGGTGA P3_COMMENT=CR is AAACGCAGTCACAGAAATCA P3_COMMENT=CM is GTGTTGCTCTGATAGCCCTT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH724 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCTGCAGANCTGGGGTTCTGATGGTTAGGATGAGCCACTTGGNNATAGATGATAGGTGAGCCATTCTAAAATCATAAAAACAAAAATCAATTCAATGAACTTAGTGAATTCATCTTACTCAGTCTGTCTTATTAAGTCTCAATGCCCCCAGGTCCTTTGCAAAAACAATGCTCATTTTGACTGCACCCTCCAGAAAAAAAGTTGCAGGAGAACTTTTATTTTGTTTCCAAGCCCACTGAAGGTAAGTTTTCTGTTTTGTATCTCAATACCTTTAACCCAAAAAGGAGTTCCCATTTCATTCCTCCTACATG SEQUENCE_INCLUDED_REGION=36,311 P3_COMMENT=CL is CTGGGGTTCTGATGGTTAGG P3_COMMENT=CR is TGTAGGAGGAATGAAATGGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH730 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGGGAACCCTATAACTGATGTGTGCTGTTTTGAGAACAAAATACCTTCTCTTTGGAGTTCTGGCTTTAGAGGANATTGATAGAACTAAAAGTACATTTGACATGTTCATTACTCAGGGAAATTAATCCATTATGGCAATTAGTGCACTCTTTTTGCACACTGAGGTCTAAATACCAACTGAAAGAAGTTATAAAGATGTGATGTTAATGAGTNTTGTGAGGTTGTCTTTTCTCAAAGAAAACTGAGTACATGAATTTAATAAAAATATTCAG SEQUENCE_INCLUDED_REGION=35,175 P3_COMMENT=CL is ATGTGTGCTGTTTTGAGAACA P3_COMMENT=CR is TCAGTGTGCAAAAAGAGTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH733 SEQUENCE_TEMPLATE=TTTTTACTTGTTTTCAGTATTCTTTAAGCCAGGAAAGTATGCCTAGTCTCAGAATTGTTTAACATAAAGACACAGAATTACGTTGGTTTCAATCCTCATGACATCGAGGGAAAAAAACCCAAATTAATATCTCAGAAAGTTATCCTGCCACTGAAGTATTAGCAAAAGTCCTTCCCATTCCATCTAGCCTTTCCATAAATGAAACTTCTACCACTTTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACG SEQUENCE_INCLUDED_REGION=0,220 P3_COMMENT=CL is TTTTCAGTATTCTTTAAGCCAGG P3_COMMENT=CR is AAAGTGGTAGAAGTTTCATTTATGG P3_COMMENT=CM is ATGCCTAGTCTCAGAATTGTTTAAC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH736 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTCCTTGACATCAACATCAGAAGTNAGGGACTTACCCTAACATCCTTGGCTTTAATAATCCATGACGGAGGTTTCATTCTTTAATCTAAACTTTTGTGGAAGNTATTCATAGTTTNTTCTCTACCGCNCTTGTAGNGATAATTAAATTGTACACATTGATTACCACTTTGCATTCAGTCATACTTTCTTTTAAAATAATAGTAACTAACATTTACATAGTGATTACCATATGCTATGCATTGTGCTAAGCACTTTACATTTCTTAAATCATTTAATATTCAGGAC SEQUENCE_INCLUDED_REGION=36,286 P3_COMMENT=CL is TGGCTTTAATAATCCATGACG P3_COMMENT=CR is GCTTAGCACAATGCATAGCA P3_COMMENT=CM is ATTGTACACATTGATTACCACTTTG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH739 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTTAGAGGCTGTATGGCCTCCAGGAAAAGTAGCTTTAATGCACTGCAGATGGCACCAAAAGTCAGGGACACCAAAAACCAAAAGAAACAGAAAGGCAGACAGAGAGGCAAAGAGGGGAGCAATGATTGCATCACATTTTAAAGAGGAAGCCTTAGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCT SEQUENCE_INCLUDED_REGION=36,156 P3_COMMENT=CL is GTATGGCCTCCAGGAAAAGT P3_COMMENT=CR is TTAAAATGTGATGCAATCATTG P3_COMMENT=CM is GCTTTAATGCACTGCAGATG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH745 SEQUENCE_TEMPLATE=GCATGCCCTGCTCAGACTGTGCACTACAGACCAATCAACTTGAGCAGTTCAGAGAACAAAATAGTCAATGTTAGCCTGGCAGATCTTAGAGGTNGAAATCGCCCCAATACAGGGCCCTTATACACAGAGGCCGATCGAGTCATACAGAGAACAAATTCAATGCAGCAGTTGGAACAGTGGATTAAAATCCAGAAGGGGAGGGGTCATGAAGANGAAACCAGGGGGTAAGTGTCTGTCTTGTCATTATGAACCCAGGTTTCTTTGATGCTGTGTTGGTGGAGATTGGTTTCTGAG SEQUENCE_INCLUDED_REGION=0,294 P3_COMMENT=CL is AGTCAATGTTAGCCTGGCAG P3_COMMENT=CR is AATCTCCACCAACACAGCAT P3_COMMENT=CM is GAAATCGCCCCAATACAGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH750 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACAGGCAAGGGCCACACCCAGAAGAGGAGGGAGGTGGACTTCAAGTGGCCTCGTAAAACAAAGACACATTCTCCTATAATGTTCAGTCATTCGGGATGTGTTTTAGACTAAGGAAGATTTCCACTCCAGGTCCTGCCAGAGNTGCTGTGAGAAACTATCACGCTGGTCCTCTCATCTGGATCACCAGACAACAG SEQUENCE_INCLUDED_REGION=33,196 P3_COMMENT=CL is CTTCAAGTGGCCTCGTAAAA P3_COMMENT=CR is CTGGTGATCCAGATGAGAGG P3_COMMENT=CM is CACATTCTCCTATAATGTTCAGTCA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH754 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATGATATGGAGAAAAACATTATCTTTCAAAGCTGAAACTTAAGCCTGTGTATTAAAATTNCTTTTCACAACTATTGATAAGACTACCTGGAAAGGAAAGTTGCCTCTATAAGAAATACTAACTGTATCATTGTAGTCAACAGTGAAAGTGAAAAAGAAAGANTTTTTGGAGAAAGCATTAAAAAGTGAAGAGAACAAAGTGTNGCAGACATTTCAACCCAAAATAACATAATTTCCAACGGNGGGTGAA SEQUENCE_INCLUDED_REGION=35,253 P3_COMMENT=CL is TCTTTCAAAGCTGAAACTTAAGC P3_COMMENT=CR is TTTTGGGTTGAAATGTCTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH757 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTGATAGGTNTTTAAATTAGAAAGTTACCTGTATTAGCCATATTTACATTTCTGTAGGCTGTACACTCTAAGAATTCTAGTTTAAAAGTCACTGGTTCATTTCAGTATGCATAGCCAGTCTCAAGTTGTCACATTTTGTTCATCAGTTTTCCTTATCTCTTGCATTTTTCAAAATTCTGTGACTTTCAAGCACATTGAACCTTCTTACTTTTACANCTTCACTATCATTAATTATTTTACTTATTATGTTAACACTGCATTATCTGC SEQUENCE_INCLUDED_REGION=35,113 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH760 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATGGGATATTATCATTCAGAGTNNTCCTGTGCCAACGNAGTCTGGGCGCTGGGAACATCTGGAGTCAACATTCTGGCCCATCACAACTCCTTAGGGACCTCCATTCCTCAGGGAATGCACTCAGATCTCAGGCACCAAAACCAACATTGTCTGCTCTTCCTTCCTTTTCTGTCTGGACTTTGTTGGCTGTTGAAAAAAGGAATGAGATTTTCCAAAAGTTTCTTCACAAACTGCTAATGCCAGTACAGGCCAGTTCCATGATTTTACCAGTTCAATCCAGTGATATAG SEQUENCE_INCLUDED_REGION=36,267 P3_COMMENT=CL is CATCTGGAGTCAACATTCTGG P3_COMMENT=CR is AATCATGGAACTGGCCTGTA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH761 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTTTAGAGANTCTCACAAGCAAAGTNTAAAATGTTTCCTATCTGGATGCTTGTGATAAATGCAGATTCCTGGGTCTCATCCCAGACTTTCAGAATCTGAATGCCTGAGATATGGCCCAGGAGAGTGCATTAAAACAAAAACAATAACANTACTCCTCCAGGTGCATCTTAAGCCTATCAGAGATTTAATGTGAGACATAGAATGTGAGAATCTTTGTTCTTGATTCCTGAGGTTATATTTTATTCAAACATAAACCCCGNTACACAACGTCCAGCATGCAGTGGG SEQUENCE_INCLUDED_REGION=36,288 P3_COMMENT=CL is TAAAATGTTTCCTATCTGGATGC P3_COMMENT=CR is ATATAACCTCAGGAATCAAGAACA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH764 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAAAAAAGAGTNNAGTTTCAATACAAAAGGTTAAAAGAAAGAAAGGTCAGAGAAGCAAGTACGATACAGCCAAAGGGCCAGGCAAAATGAAGGTAAAACATAGAGACAAAATCGAGAATCAAAGTACGTTTGAGCATAGGAATGTCCTGATGAAAGTGGCACTTACTGAATATGAATATTGAGGCTAGTTTCAAGGGGACACACATAAGGCCAAGAAGTCAGATGGGAGGATGTCATAATAATCTACATATAATATGGTA SEQUENCE_INCLUDED_REGION=35,261 P3_COMMENT=CL is AAAGAAAGAAAGGTCAGAGAAGC P3_COMMENT=CR is TGACATCCTCCCATCTGACT P3_COMMENT=CM is AGTACGATACAGCCAAAGGGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH765 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAATATTTGATCAAGGAGAGAGTCTATGTCCTTATACAGGTTAATTAGACAATCTGATAACAGTCCTATTTGTGACTAGTTACTCTGAGTNCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT SEQUENCE_INCLUDED_REGION=36,94 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH766 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGTGTNTTTCTCCCTGCCTGGAGCCTGGAGCCTGGGGTCTGGGCCAGGAATTCCCAGGCTAGGGTTGGACTCCAGGTGCAGGAAGCAGCCTGAGGCCCTGTGCAGGTCCAGATCTTCTCAGCCAGCAGGGTATGCCCCCACACAGAGGAGAGAGTNGACTGTGGGTGCCAGGCTGGGGGTGCCTGCAGAGCCTCCAAATCAGTGAACCTCTTCTCTGAGGGTCTCTGTAGGGGGGTGTGAACTTAAACTTGAACTTAAATGAGAGCATCTTAAGCCAGAGGACCAAGTTTTAG SEQUENCE_INCLUDED_REGION=35,164 P3_COMMENT=CL is TTTCTCCCTGCCTGGAGC P3_COMMENT=CR is CTCTCCTCTGTGTGGGGG P3_COMMENT=CM is GGGTCTGGGCCAGGAATT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH767 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGGTGCTGCAGTATCAATCCTCTGAGTNNACTAATATTCCGAGGATTAATACTCAGCCCTCTTGGGNTCNTAGATTTCTGGACCTACTTTGAAATGAGTCTTATACCCATCAAAATATAATGAAGAGGGCATGTCTGTGAAAATATGAAAACCATTCTTATTTTCCATATTAATGAGAAAAGTTAATTTATTTCTCACACTGGGACATTTTAAGTGAAAGGCATACCTTCAATGATACCATATAAATCTTGGATAG SEQUENCE_INCLUDED_REGION=36,258 P3_COMMENT=CL is GGTGCTGCAGTATCAATCCT P3_COMMENT=CR is TGGTATCATTGAAGGTATGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH771 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAGTNGGGAGAAAAAAATTACCTCAAACCCTACCAAGTGTCAAAGCGGCCTTAAAATATTGGTATTTAAAGGAGGTATAATACCAATAAGTCTGAAGTGATTAAGAGGTCTGAGTNAGGACATACAGGGCTGTGTTATGTAGTTTGTGACAGAAATCTTAAATTAAGACGGCAACATAGATGGTGTAACTTACTCGCAGACCTGAAAAAAAAGTGCTGTCAACCCCTCAACTCATGGTGATGGCACTTAGTGAGCATTGGTCTAATAAAGGGGCAAAGAGTGTATGAGTTTCTCCAATTCTGATTTTTTTTTTCTGG SEQUENCE_INCLUDED_REGION=36,192 P3_COMMENT=CL is AGCGGCCTTAAAATATTGGT P3_COMMENT=CR is GTTACACCATCTATGTTGCCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH777 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCCTTCCAGGGAGATGAAGTTATTGAACATCCACTTTAGAAATAGAGCACCATGGCAAGTAGTGCTCTCTGAGGTTCAGGATCCTCTGCAGAGTNNATAATACATCTAGGAAGACATCAGGCTTTAAAAACTCTATGGAGCAACATGTCAACAAATTTGATTTAATATAGGAGAAAAAAAAATCCCCGCATATCTTAGTGCTGAGTGGACACTCTAG SEQUENCE_INCLUDED_REGION=33,217 P3_COMMENT=CL is CCTTCCAGGGAGATGAAGTT P3_COMMENT=CR is ACTCAGCACTAAGATATGCGG P3_COMMENT=CM is TGAACATCCACTTTAGAAATAGAGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH779 SEQUENCE_TEMPLATE=CTGAAGGTAAAACTTCTGATTATGTTTGAGAATTTAAATATTAAACGGACACAAATGACATTCTAAATACTTTCTCTTTCAGTTTTGACTTTATTTCTGACCTAATTTTCTATTTGACAAAACTTAGAGTGATTTATGGTGCTAAATCTCATTTAAAAAGAATAAATTCACTAAAGCAAGTTCCATACAAAAATCAGTATCACACATCTTGAGGGCTGTTACATCACTAG SEQUENCE_INCLUDED_REGION=0,230 P3_COMMENT=CL is TGAGAATTTAAATATTAAACGGACA P3_COMMENT=CR is TGATGTAACAGCCCTCAAGA P3_COMMENT=CM is TTCAGTTTTGACTTTATTTCTGACC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH780 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTATAATAAATCTTGAAATCGGGCAGTATAAGCCCTCCCATTTTGTNCTCTTTTTCAAAGTTCTTTGGCTGGTTTATTTCCTCGGTATCTTCATACAAATTCTAGAATTAGATTAGTTCAGCTTTTGGTTGGGGTTGCACTGAATTGATAGATCACTTGTGGAGGTTGGTATCTTGTTAAAGGAAGTGCTTGGAGGGTACGGTCTAACTTTGTTCCTGAATTTAGGGGTAAACATTCAGTTTTCACCCTTGTGTATACTGCTAG SEQUENCE_INCLUDED_REGION=36,196 P3_COMMENT=CL is TGAAATCGGGCAGTATAAGC P3_COMMENT=CR is TCTATCAATTCAGTGCAACCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH781 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTGCTATTTATTAGTAATATAAATGGTCAATGTATGCATTCATTATGCACCAAATAATAACTTCTGTACATACATTATTGTATTTCATTATCACAAAATTATGAGTGAGGGATGATTGTTATCCCTATTTTACAGATGAGAACACTGAGACTTAGAAGAAGTATCTTTCCCAAAGTCACAAAGTTAGTGACAGAGCCGGGATTCGAATCCATCAACTTGAATCCAGAGAAAATGTTCTGCATCACTGTACAACACTGACTCCTTTTTCTCCTTTGAAAACAAGGCAAGATGCA SEQUENCE_INCLUDED_REGION=34,292 P3_COMMENT=CL is TGCATTCATTATGCACCAAA P3_COMMENT=CR is TTGCCTTGTTTTCAAAGGAG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH787 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGGAACCCACTGACTATAAGGCAGCCTCACTCCTAGGAGGAACATCACAGCAACTCGCTAGCAATTGTAAAGTGTAATGATTTTCCCAGTCCTTCCCACCAAAATCTATGCCACTGACTCAGGGGGCTACACACTGAGAAGATGGAAATACCCAGACATTTCAAGGACTACTGGACACAGGCAGGAGTTGAATTTGACACTTGGGGAACCAATGCATCCTCATGGCTCCCCTGCTAAAATGGTAG SEQUENCE_INCLUDED_REGION=36,248 P3_COMMENT=CL is GGAACCCACTGACTATAAGGC P3_COMMENT=CR is CCCCAAGTGTCAAATTCAAC P3_COMMENT=CM is GCCTCACTCCTAGGAGGAAC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH790 SEQUENCE_TEMPLATE=GGGAAGGTGTGGGGGGCTGCTGGGGCACAGTCATGGGGAACNCCAGTCCCCCTCTCCATGTGCTTCCCTCAATGCTCCCTGATGCCAGCAGTNCTGCCCCCGAACAGAAAGGGGCATTCCTGTGAGTTCCTTGTGTGGGGGACTGGTCACAGAGACTCCCCAAGTNCAGGGCAGGGTGGAGGGAGGCTGAGGGGTGGTTGAATGGACAGGAGAAGAG SEQUENCE_INCLUDED_REGION=0,217 P3_COMMENT=CL is CTGCTGGGGCACAGTCAT P3_COMMENT=CR is TCTTCTCCTGTCCATTCAAC P3_COMMENT=CM is CCAGTCCCCCTCTCCATGT P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH795 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTCAGGGNCAACCAGAAATTCATAGCACAGGAATGGCCGGCAGCAAGGGGTCTGAGAATGTCAAAACAAGCATCCTGGAGGGAAGGCAGATGCCATCAGACTTCTCCAAAGACTCTTTGTACAGACAGTATTTCAGACCATCCCAGGCATCAACAGGAAGTGAGTAGTTACCTTCTGATTGCACGTATAAGGTGACTGGAATACAAAATGCTGGGTTTTTGGCCCGAGGNCCCATGAAGAGATCATAGTTCAAATTTCAACATGTCTAAATTCAGTTCTAGCCACTATGGCTTGCTAGTGTTACCTCAAAATAGAGACATTTATTTTCCCTAA SEQUENCE_INCLUDED_REGION=36,334 P3_COMMENT=CL is ATTCATAGCACAGGAATGGC P3_COMMENT=CR is AGGGAAAATAAATGTCTCTATTTTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH799 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTGCAGCATTCCATAATGTGAATATATAACACTTTATTCAAAATTTGGGGAAATAGTACCTTGTACATACATAATTTCACATGTTTGCCAGTGTGTGTTTTAGATACATGCCTGGAAGTGAAATTGCAGGTAGCCTGCATATGTTAATTTTGCTAGGTATTACCAAACCAG SEQUENCE_INCLUDED_REGION=36,173 P3_COMMENT=CL is CTGTGCAGCATTCCATAATG P3_COMMENT=CR is AACATATGCAGGCTACCTGC P3_COMMENT=CM is ATAACACTTTATTCAAAATTTGGGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH800 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGCTTTTTGTTGTTATTGTCAGTTTGTAACAAACACCATCCTGCTTAGTNTGCACCCTACACTGCTCTAAGCACCTTAATAGGAATGCATTTAATCATTGTAAAAATCCTGGGATTCTATTGCAGGCTTAGGTGACTCAAAAGCCACTCTTTCCACGACAACCCTATTAAAACAAGAAATGTGAATAAGCCATGTTGTTGCTATGCGCTTAGTGAGTCCTCAGTAAATGTTAG SEQUENCE_INCLUDED_REGION=34,234 P3_COMMENT=CL is TTATTGTCAGTTTGTAACAAACACC P3_COMMENT=CR is CTAAGCGCATAGCAACAACA P3_COMMENT=CM is TGCACCCTACACTGCTCTAA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH803 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCTCCTCAGAGAGNATTTGCATTTGNCTTTTTCAAATCACTGGAGGAATTATTTATATCAGACTACTACAGTTTAATGCCACGCTTTAAACCACACTGGTCATGTGAATATTCTATTTAATTTTACATGATGCTTAAACTGTGCTTTTGAATATTCAAGGTATGTGTGTGTGCGCATGTGTATGTGTATATGTGTGTGTTTACATTAGCCACAAATTAAAGACAGATAAATTTTCTTGCTGACCACTTCTGTGCAATAGG SEQUENCE_INCLUDED_REGION=36,264 P3_COMMENT=CL is ATCAGACTACTACAGTTTAATGCCA P3_COMMENT=CR is ATTGCACAGAAGTGGTCAGC P3_COMMENT=CM is ATTTTACATGATGCTTAAACTGTGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH805 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCACCCAGAGCAAGGAAAGATCATGAAAACCCAAGCAGAAGCAAGACACTGGGTTCCAGCATCAATGCTAAGGGCTAGAACATTTTCTGATGTTTTGCTTGGAAAGGCAGAATTTGGTTGTCTAGTGCGTCCACTAATTGGCCTCCATCTGACACCCTGGAGGGATGGGCTGATTAGAAGCCACAGCTGCTTTGCTCATTATTTCTGATGAATGGACATCACCTGGCGTCTCCGGTGATGGCGCCAG SEQUENCE_INCLUDED_REGION=34,248 P3_COMMENT=CL is AGATCATGAAAACCCAAGCA P3_COMMENT=CR is CAGGTGATGTCCATTCATCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH806 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTAACCTAATATGTACTCTGGGCTGACTTCCATGTTATAACTGTGTGCTTCAATGCTGGCCAAATTTAATCATGCTATAGTTTCCCTCACTTTTCAATTACATTTTTTATTTCCTTACTCTGGACCACCATGACTGCTAGAATTGTCTGCGTGCATTGTCCAAGCCAG SEQUENCE_INCLUDED_REGION=36,171 P3_COMMENT=CL is TGGGCTGACTTCCATGTTAT P3_COMMENT=CR is CAGACAATTCTAGCAGTCATGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH813 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTAGCACTTGTTGGGCTAGAGGGTCATGGTGGTNGTNAGGGTAGTTAAATTTTTGTCATGTTTTGAAGTGTGAAGATTTTCCAACCTTCAAAGAATAATAAAGAAGGGAGTGCCCCTCCATAGGTCAAGCAAATGTACAATCACCCAGAGTNATAAAATATGTAGCCTTAGGTGATTGTCAAGTAATTCCCTATATAATTTTTATAAAGATGAGTTTCCCATTGGAACATTTATAAATGACTTATCTATAAAAATTCATTCACCGCACCAATCTTAGG SEQUENCE_INCLUDED_REGION=35,279 P3_COMMENT=CL is ACTTGTTGGGCTAGAGGGTC P3_COMMENT=CR is TGTTCCAATGGGAAACTCAT P3_COMMENT=CM is TAAATTTTTGTCATGTTTTGAAGTG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH820 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGTTGCTACCCTCGTTCCGATGCTGTCTTTCGCTGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=36,33 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH822 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCTGTATATTCCTGAACTCTGTAGACCTTCCTGACCTGTTTGCAGTGGATACTGTTTTTGAAGGCTCTGTCTCAGTATCTGAAGTTTTTGTCTCCAGAGAAGTCATTTTGTAGGTGTTCCTGGGCGTTTTTGCTACGTTTCCATTTTCTCTAATACACTGCCGTCTTAAGGGAGGGCTTGCAGAGCATTTATCAGATGGCTGTTTTGCTGCATTCTGTGCACTGAAGAACACATTTCCTCCTGAAATAAAAACATACACAATAAAAACATTCTATTAGTGTCTCATGTCAATCA SEQUENCE_INCLUDED_REGION=36,296 P3_COMMENT=CL is CTTCCTGACCTGTTTGCAGT P3_COMMENT=CR is CTTCAGTGCACAGAATGCAG P3_COMMENT=CM is GATACTGTTTTTGAAGGCTCTGTC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH830 SEQUENCE_TEMPLATE=CATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTGGGAGAAACAAAAGAGTNNAAAGAGAGACCGAACTCAGAACCGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGTAATAGTCATAAATCAAAATACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCAAAGAAGTCCAAAGATAAAGAAAAATCTAAGTATAGATGAAAGATGAAGAGGCAGAATTGAGAGGCTAACATATTTACTCTTGTCTAACTTAAGAGTGCCAGGAAAGCAGATGCTTAGATTTTGTGTCAAAGCTTGTTATTTTTTTCATACTAGGATTATGGTCTTTAG SEQUENCE_INCLUDED_REGION=31,334 P3_COMMENT=CL is AGAGAGACCGAACTCAGAACC P3_COMMENT=CR is GACCATAATCCTAGTATGAAAAAAA P3_COMMENT=CM is AAGTCGTAGCCGATCTCGAG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH832 SEQUENCE_TEMPLATE=NTTNCNNCAGNTCGACTCTAGAGGATCCCCCTCGTATAAAAGCACCAAGACAGGAATCAAATTGCTCTATTCGGACCCTCATATTAAATAAGAGCAATGAGAGCGAGGGAAAATTGAACTCTCTCAGGTACTGACTGTGGGACCAGACAAGGGATGTAGATTGTCACATTCAATCCTGAAACAAACCTGCCAGGCAAGTCTTCTTCCCATTTTACAAATAAGGAGACAAAAATTAGGAGATTAAATAACTCATCACTGTTTTCAAAATAAGGAGTGTGTGAGGTTTTGTCCCAGGCTCCTGAAGTTTCTGCCTGG SEQUENCE_INCLUDED_REGION=30,285 P3_COMMENT=CL is AAATTGCTCTATTCGGACCC P3_COMMENT=CR is GGGACAAAACCTCACACACT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH835 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAATATGCACTGAGCACTAGACAGTGTGCTAAGTACTGAAGATATACACTAAGATAATCCTCACAACCACCGTATGAATTGATGAGGAAACTAAGCTTTAAAGATGTTGATATTTATAAGGTATAGTGTTTCCTGTTCATGTGTTTGAG SEQUENCE_INCLUDED_REGION=34,150 P3_COMMENT=CL is CTAATATGCACTGAGCACTAGACA P3_COMMENT=CR is CACATGAACAGGAAACACTATACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH839 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGGGATNTNNTGATGATTGTTGAGATTGAAAAAAAATGGCAGTATCATGAAGAGGTNATAATAAATTCTTATTACAATAGTATTAAAAGTGGTTTTTCACTCAGTCAGGGAGAAAAATTAGATTGTACTTTTTGTCCTCTATGATTTCCTTCAGTTATCTTCTAAATGTTGTTTTTTCCCCACAGCCCCCTTAACATTGTTCTCTACGCACTTCTCAATATACACTTTCATTTGTTTCTCAAGCTTCTTTGTGGATGACTCCTAAATATAG SEQUENCE_INCLUDED_REGION=34,274 P3_COMMENT=CL is TTGAGATTGAAAAAAAATGGC P3_COMMENT=CR is TGAAAGTGTATATTGAGAAGTGCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH842 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTATGCCAAAGAATTAAAAACACACAGAATCATCAAAGCACAATCTGTGTTTGAGATAAATGATAGTCTGAGTCACCTATGTAAGAAGTAACTCTGAAATAGTAGGATAGTATTATCATTTCCTGTAATAGATTCACCTCTCAGCAATTGGTCTGTTTTCATTCTATGGAAACTCTCCGTACTGTAATTTTCATTCTATGGAAACTCCCCATACTGTAATTGGACAGTTTTGGTTTCCACACCAG SEQUENCE_INCLUDED_REGION=36,246 P3_COMMENT=CL is AAACACACAGAATCATCAAAGC P3_COMMENT=CR is GTGGAAACCAAAACTGTCCA P3_COMMENT=CM is TCATTTCCTGTAATAGATTCACCTC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH851 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTCTGAGAAGGGGAAAATCTGTCTGCTTAAAGATCTCACTAAACAAGTGCTGTACAGTGACAAGTTACTTATTTTCTAATACCTTTCAGAGGCAGATGACGTATTTTATTTTCCTCTGAGGTCAACAAAAGAAATGGGGTATTATCTGATGATCTACTTTAACATTCTCTATATGAGAAACAAGAGTGTCTAATGTAAAACACAGGATAATTGTGTGTGTGTATGTGTATACAAGCATAAAATGTTTCAGGAAATAATTTCTATAAGTTGATTCTGAAAATAAATCAGTAATAAAACAGTTA SEQUENCE_INCLUDED_REGION=33,304 P3_COMMENT=CL is AAGGGGAAAATCTGTCTGCT P3_COMMENT=CR is AATTATTTCCTGAAACATTTTATGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH861 SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGTGAATTTNCCACAGCCCCAGGGAGAACAATTATAGTGACCAACATTTACCTTTGCTGGAGACTTCCTGAATGGGAGCAGGGGCAGATACAGGGACCTACAGGACTGAGACTGCATAGAAGATGCTGAAGAAGACAGATGATTGCCAAGAGTTAGATCCCCCTCCCTACCATCCCTGGAACTTGGATGCAACCCTGAAGAAGATAGGATAAAAAAATGCTGAAGTCAATGAACTCACCTACATTTAGTAAGATTAGTTTCCTTCTGTCGTTTTTGCCTGAGGAG SEQUENCE_INCLUDED_REGION=34,289 P3_COMMENT=CL is CAGGGAGAACAATTATAGTGACC P3_COMMENT=CR is TTGGCAATCATCTGTCTTCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH862 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTATTTGTATTGCTGGAAACAATAGGATAATTTGCTGAGGTTGGGGAGTTTCTCCCNTCCAGAGAGTNCTTAATCTTCCAAAATTTGGTTGAGATCCAAGGTTGATTTTGCTCTACGACTTTTTTTCTGAAGTTTTACTCATTTCCAATAAGAAGGCATGTTTTCCTGCTTCCATGATGATGGAGAACAGGCACATCCTTTCTTGAGTTTCAGCGTGCTTCTGACAGGGAGGGTGAGTGTGAGATTTTTCCTTCTTGTAAGATGGTAGAGAGCCATCATCAGCCTGAGCCATATTTCCAAGTA SEQUENCE_INCLUDED_REGION=34,302 P3_COMMENT=CL is TATTTGTATTGCTGGAAACAAT P3_COMMENT=CR is ACTTGGAAATATGGCTCAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH864 SEQUENCE_TEMPLATE=TGCCTGCAGGTCGACTCTAGAGGATCCCCTTCAGTGATAAGGACAGGTCTAGAACAAGCGTTCCCAACCCTGGCACCAATGACAGTTTGGACCAAATAACTCTTTGTTTCAGGGGACTGTCCTACACATTGTGGGATGTTTAGCAGCCTCCGTGGCTTCTACCCACTAGATGCCAGCAGCACAACACCCCTCCCCAACAATCATGACAATGAAAATGTCTTTAGACATTGCCAAATATACCTTGTGGGACAAAATGGCCCCTGATTGAGAACCACTGGTTTGGAGTATGGTTATGAGAAGTACAGTCTTGGCATGTGAAGTTCTGGAAATC SEQUENCE_INCLUDED_REGION=29,302 P3_COMMENT=CL is TTCAGTGATAAGGACAGGTCTA P3_COMMENT=CR is TTTCCAGAACTTCACATGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH866 SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCGATGGCTCCGACGGCTTGTATTCCAGCACAGCAGGGCTTGGCCGTTGTTGCAGACATTTGCCTTCTCTGCNTGGTGGCATGGACTCCATCCAGGACAGGTGTTTGGTTTCGTTTGCTGGGCCGTGATGGTGGAAG SEQUENCE_INCLUDED_REGION=33,137 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH870 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAAGATGCAGGCGGAAGGTTTTTGTGCTGGAGACAATACAGGGGATGACTTCTTAAATCCGTCGTCGTCATCCTCCATTAGTGATAAATAGTCACCTTGGCTCAGATTCGTCAGACAAATACTATATTGCTACTTGTTATCTTCTAGTAACCTTCAGAAAAACTTTAGTGTTTACTTAAAAAATGAGGAAAAAAAAGAATCATTATGTAGAAAAAAATGAGAACATTTTGCTTCGTTTCAAAAATTATTTATTCAAATTAGTGAACAAAACATGGTCTATCGGAATAGGCTCATTAAACAG SEQUENCE_INCLUDED_REGION=34,303 P3_COMMENT=CL is GAAAGATGCAGGCGGAAG P3_COMMENT=CR is TGTTTAATGAGCCTATTCCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH877 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTTGAGATCAGTCCTCATCTTATTTTATATCCTCAGCTTCTAACATGTTGTCTGGTGCATAGTTGGCACTAAATAAATATTTTTGAATGAATTAAGTAATGTCAGCAGATGACTAGATATTATATGCTTCTTGAATTCTTTCCTAATCTCTTCAACCACCACACCTATCTCCCACCCTACTGAGAGTTGAGTATTTCCTCTTCTGTATGTCAATGAAAATTTGCAAGTAACTTAGGTGACCAATCATATACCCAGGTTTACCTGAGACATCCCTAGTTTATGCCTATTGTTCTTGCATAACTATT SEQUENCE_INCLUDED_REGION=33,308 P3_COMMENT=CL is CTCCTTGAGATCAGTCCTCA P3_COMMENT=CR is AGTTATGCAAGAACAATAGGCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH880 SEQUENCE_TEMPLATE=TGCCTGCAGGTCGACTCTAGAGGATCCCCTTTTCGTTTTGGGGTGGAAAACTTGTATCTGAAATGTTCCTGTGAATGTTAAGAAAGTTAAAAATGAATTATTTTTCTCTTCTTTAATGCCCATCCATTTTTTGTCTTCATCTGAGAATGACTTTCCTAAAGACAACATTCGTATACAGAAGCCAGACCTATGCAAGAGAAAGTATAATAATTTTCAATGAGGCAAAGGTCATTAATATGACCTACATCTTCATAAAATGCGAATTTAATCACGAGTCATGACGATTGTTCTTTGAGAGGCTTTTTATTCCCCATGGTGAAAAAAGATTTGTAGT SEQUENCE_INCLUDED_REGION=29,305 P3_COMMENT=CL is TCGTTTTGGGGTGGAAAACT P3_COMMENT=CR is TACAAATCTTTTTTCACCATGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH881 SEQUENCE_TEMPLATE=ATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCAGTTTCAACTGGAGAGATCTAGATAGCATTAAGTGTTTATTTAGGTGTGGCAGACTGAATCCTCTTGACCTGTGAACATATACTATTTGACTAGAGTNATGTTTTGCCTTTTTAAATTAAATTTGAGTAGTTTTGGTTGGAGCATTACTACTATTTGCAACAATCCTCAAAACTCCCTATTTATTGCTTTTTGCTTGGCTCACTTCACATCCATTGTAGGCATTTGTCTGTGTTCTGGTTTAATGCTGTGAGTTTTAATAAGAGTTTCTGTACGGAGCCTGAGATATCTCTATAAGAAGAAAAA SEQUENCE_INCLUDED_REGION=30,272 P3_COMMENT=CL is TTTCAACTGGAGAGATCTAGATAGC P3_COMMENT=CR is GAAGTGAGCCAAGCAAAAAG P3_COMMENT=CM is TTAAGTGTTTATTTAGGTGTGGCA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH889 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCTCCTGTTTGCTCATTTTGAAGAGACTCATTACGGACAATGGTCCTCAATGTTAATGAAGTGACACAATCCAATTAGAAGATTGCAGTTACACATGGTAGTGGTAGAATGAGAGGANTTGCTACCTAACAGGTAATGTCACTGTTTGTGCCTTGTTTTTGCCCTCTGTGGGGTTGTTTCTGCTTTGGTCCAGATCTCACTCTGTCCTTGGGCCTACTTTGGGATTCTGCCATCTTCTGAATGCTGGTAAATGCCTAAG SEQUENCE_INCLUDED_REGION=36,262 P3_COMMENT=CL is CTCATTACGGACAATGGTCC P3_COMMENT=CR is AGGCATTTACCAGCATTCAG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH901 SEQUENCE_TEMPLATE=AGGGAAACTTCTCTGTGAGANNAGGATCAGGGCATGGCTGGGTGGGAAAGCCAGGAGGGTTTCCTGAAGGAGACATCACCCTAGTCTAATAATTAGCTTTAATTATCCTCACCACAGCCCTAGAAGGAAGGCACTGNTTATCTCTCATTTTACTTTTCTCTGAAGTTAAGAGAAATTAAGTGACTTGCCCAAGGTGATGGAG SEQUENCE_INCLUDED_REGION=0,202 P3_COMMENT=CL is GGGTTTCCTGAAGGAGACAT P3_COMMENT=CR is ACCTTGGGCAAGTCACTTAA P3_COMMENT=CM is ATTAGCTTTAATTATCCTCACCACA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH903 SEQUENCE_TEMPLATE=CATGGTGTACTACCTGGGTNTTGATAATGGTTATTCAGGGACCAAGGGCACTTTAGTCAGAAGGTGATTGGTCCTGCCAGANCTGAGTCCATCGCTTCATGGCAGTGGGTTCCCTTGTGGCCCAGGGTGTGTCTAGAAATGTTGTCCAGGAG SEQUENCE_INCLUDED_REGION=0,152 P3_COMMENT=CL is TTGATAATGGTTATTCAGGGA P3_COMMENT=CR is CCTGGACAACATTTCTAGACA P3_COMMENT=CM is CAAGGGCACTTTAGTCAGAAGG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH904 SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGATTTTACTGCTTTTNCTTTCTGTTTAACTTTCCTTTCCTTTTCTCCCTTGGTTAGAGTGTGGGACACTAGATCAGCAAAATTAACAAGATCATGAGTTTGAGAGGTAGCCCAAAGGGGGCATTCATTGTCTCTTTATTATAATTGCCAATTCTTCTTCCAATCTGTTTACAAAGTTGAATAAAGGGGATTCAAGTTGATTTTGGTATGATTTTGGTGGTTAGTATAACCTTCTTCTGATAGGCCTAAATGTTATTTAAAGACTTTTGTAAAGATCTTTTATAGTATGACACTACAGGCTC SEQUENCE_INCLUDED_REGION=34,303 P3_COMMENT=CL is TTTCCTTTTCTCCCTTGGTT P3_COMMENT=CR is TCAGAAGAAGGTTATACTAACCACC P3_COMMENT=CM is GAGTGTGGGACACTAGATCAGC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH909 SEQUENCE_TEMPLATE=TCGACTCTAGAGGATCCCCCTAACTGCTCTAGTATNCTAGCCCTGAAAAACCACAAAGATGAGGATTAGCAGATGCTCAAGAAATATTTTTTGAATATGCTGGCTCTTATCTGCTCAGCAGTGTACAGATATGCTCTCACACTCGAGAAGCTACCAGAACAAACTGGTAGGCTCCCATTCTAGATGACATCTTTTCCCCATACTCTCAAAATGCAAGTTAACCCCCAACCCTGAGCAGAAGAAAGGAGAGGCCCCAGGTACCTGTCAATGAGGGAATCCCGCATGCTGGTGGCAATGGTGCTAGGCTGGGCTTCATTCAG SEQUENCE_INCLUDED_REGION=19,301 P3_COMMENT=CL is CTAGCCCTGAAAAACCACAA P3_COMMENT=CR is CTCATTGACAGGTACCTGGG P3_COMMENT=CM is GATGAGGATTAGCAGATGCTCA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH915 SEQUENCE_TEMPLATE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTAAGATAAGAAGATACCACTGTAAAAAGGTTATTATTCCTAAAGAGGAACTAAACGTAGGCAAGGCCCCACTTTTCTGGAAGTGGCTTATCAGTGTGGATCCTAATAAGAACGAATGTNTAGCAATCACTTATATCCCAGACATGATTCTAAGTTCTCCATGTGCATTTACTCCTTTAATTCTCACAACACTCCTTGCAAGTGAGAAGTCTTATTATTTCCAAATTTCAGATACAGAAACTCAG SEQUENCE_INCLUDED_REGION=31,245 P3_COMMENT=CL is ACCACTGTAAAAAGGTTATTATTCC P3_COMMENT=CR is TGAGTTTCTGTATCTGAAATTTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH917 SEQUENCE_TEMPLATE=TCATGCTCAGGTCGACTCTAGAGGATCCCCCTGGGGGTAAAGGTGGGAGGCAGTAGATGGATATAAAAGACCAGTGCTGCTCTCCCCCAAGTCCCCTCCTTTACCATTGCTTTCCTATGAATATTTTAAAGCAAAAGTAAAATAAGAATACCTAGGGCTTAATTTTTAAAGAGGGCACCTCATTTTATCCAAACCTCAGTAGGTACAGACCTGCTGCTTGGACAATATCACCTCAGCAGAGGCAACACTTCTGATGGCCACACTCCTAAATTTTAGTAAAAATACCTAAGAGGAAGCAATGAA SEQUENCE_INCLUDED_REGION=30,273 P3_COMMENT=CL is TGGGAGGCAGTAGATGGATA P3_COMMENT=CR is ATTTAGGAGTGTGGCCATCA P3_COMMENT=CM is AAAAGACCAGTGCTGCTCTC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH918 SEQUENCE_TEMPLATE=ATGCTCAACCACTCTCCTCTAACAGCCCTGTGAACTTCCTCTGGCTTAGAATACATTGACCTGACTCTGTTGTAATTGCCGGGTTTTTNATTTGTCTTCTCCATTAGACCTCGGTTTCCAAATCTCGGCACAATTGACATCTTGGGAAAACTCACTCTTGGCTTGGGTGGGTGATAAGGGACTGTCCTGTGCATTT SEQUENCE_INCLUDED_REGION=0,196 P3_COMMENT=CL is TCTCCTCTAACAGCCCTGTG P3_COMMENT=CR is ACAGGACAGTCCCTTATCACC P3_COMMENT=CM is ACTTCCTCTGGCTTAGAATACATTG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH919 SEQUENCE_TEMPLATE=NNTTCANTNCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGGTAGCCTTTAAACTAGTGTAGCACTGAAGTATGTTTGCTGTGTTTAGTTTANTATAATTGAATTTTTAACTTAAAATCTTACTGTCTTATTTGGAAGAAGCACCAGTTCAAAGCACATAGGTCTACGTTGATAAATGAATAACGCTGATGTTAAGCACATTTATTATGAATGTNATTTCTCAAGATGCACCTGCTGCAGCGGAGAAGAATCAGTCCTGGTCAAAGTGAGCAGTAGGTAGAG SEQUENCE_INCLUDED_REGION=35,247 P3_COMMENT=CL is AGTGTAGCACTGAAGTATGTTTGC P3_COMMENT=CR is AGGACTGATTCTTCTCCGCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH936 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCAGCCTTTAGCCCCTCTCCCTTCACTGGAGGTTGGGGCTGGCGGGAGGGGTGGGCCTGAAACTTCCAACCCTCTAATTAGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGT SEQUENCE_INCLUDED_REGION=36,82 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH952 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCTGTTCAGGTACCCCTTCCAGAGAAGCCAGGAGCACCCGGCGTCCCANACAAGTAAGTAAGCGCTGGGCGGGGGCCTGGCGCTCGCNGGGGCCGCCTCATTCCGGGGTCGCCTCATTCCAGGGCCGCCTCGCGAG SEQUENCE_INCLUDED_REGION=36,137 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH958 SEQUENCE_TEMPLATE=TTGCTCTGCCTGGAGCATCCTCTCTGACGTGGCTCTGTGATTCACTTTGCGCCCCTCCTCAGGTCTCTTCTTCTCCGTTACCTCCCTAGACAGGTTTTCCCTCTGCCCTGCTCTCAATCACTCACTAGTGTTATTTTTCTTCATGCCATTTGTATTCTCTCCTGTCTTCAATTGCTTATTGCTTATGTGTTTATTGTTACCCACCCCAACTGGAATGAAAGATCCATGCAGACAAGGACTTTGTTTTTGCTCACTGCAGTGACTCCACCACCTAGAACAGTGCTTGGTG SEQUENCE_INCLUDED_REGION=0,289 P3_COMMENT=CL is CTTCTTCTCCGTTACCTCCC P3_COMMENT=CR is AGCACTGTTCTAGGTGGTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH959 SEQUENCE_TEMPLATE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTTTAAACAAACCTGCAGTAGAAGCAAGCCCTCTCCAGGAAATCCCAATCTCTTTCCAAACAGGGACAGCAAAGGTTCTCTCTGTTGTGGCTGTTTTTCCCTCTCTTCTGGGAATTGGTTTGCCTTCTGAGAGCATCCCAGGAAGGGGAGGTGCGCCCCAAATTGGCGAGGCTCTGCAAATACCAAAGGCTCTTCTTTGGAAGAAGACTCCTCCTTTGGACGTGAGGCGCCCCTACCAGAGCGCCCGCNTACAGAAACGGAAACCACTAGCTACCTGGGAGG SEQUENCE_INCLUDED_REGION=33,283 P3_COMMENT=CL is AGTAGAAGCAAGCCCTCTCC P3_COMMENT=CR is ACGTCCAAAGGAGGAGTCTT P3_COMMENT=CM is GGAAATCCCAATCTCTTTCC P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH963 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTCTTTTCATGCTGTATCATTTTTAATAACTCAGTTAAAAGTTGTCTTTTTAGAATGCCTTCTTATGGTNTTATGATCTGGCCTCACTTCGCGAATACTAATTGAAAGAGCCAAGATATGGTTACAGAAGGTCCAGATGAATGGAGATGTGTCACTAGCAGAACAGAGTTGACTTTCTCCATGGGAATCTATCCCTGAAACAAAGGGACTGTCACTTCAATGACCCATGAGCCTCAGTTAGTCACTAAGCAGACATATTTGAGAAACATGCCTTATCAATTACTTCAGGAAATAAGTATTTTTAAAAAATGAAAATTTATTAACT SEQUENCE_INCLUDED_REGION=34,328 P3_COMMENT=CL is GCCTCACTTCGCGAATACTA P3_COMMENT=CR is TATTTCCTGAAGTAATTGATAAGGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH968 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCACATATATGAAGTNAAGATGGCAGGTGCCAACCTGTCCACTTCTTGGCAGAAAATGATCTCAGTCACCAAGGGAAGGGGAGGCTCAGAGCAACTCTTCCTGGGAGTGTCCTTAGGACAATGACTGTTGGCTCACCTTGAAGTACACAGTGCCCCGCTCTTTCACTATGGTGCTCTGTTCCAG SEQUENCE_INCLUDED_REGION=36,185 P3_COMMENT=CL is GTCCACTTCTTGGCAGAAAA P3_COMMENT=CR is AGCACCATAGTGAAAGAGCG P3_COMMENT=CM is GATCTCAGTCACCAAGGGAA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH974 SEQUENCE_TEMPLATE=CTGAAGGAGAGGAACCTCCAAATACAAACCTTGTTGGTTTTCGTGATTCTTGTGGCATTCAATGTTAAATGCTTCTGCCCCGAGGCTTGTGGCAATTGGTTAATATTGATGTCAGTGCCCTTCCCTCCTGTGTATTTTCGCTGGGAAAGTTTGAGAGATAAGGGGAGTTGATGTGTACAACTTAGTTTGAAAAGAACTTGTTCTCTCCTAGCCACTGAAAACCGCGTTGCTTTCTGCTTCCCATTCTTCCTTTACTGATAATCTGGCCATCACTCTGCTGTTTAAAAGTCTTGCTAGAGTATGGAGACAAG SEQUENCE_INCLUDED_REGION=0,311 P3_COMMENT=CL is GAAGGAGAGGAACCTCCAAA P3_COMMENT=CR is GTCTCCATACTCTAGCAAGACTTT P3_COMMENT=CM is ACAAACCTTGTTGGTTTTCG P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH976 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGCCCCTCTGTGTTGCTTTAGCCAAAGGTTAAGTTTACCTCCTCCAGGAGCCTTCTCTGATAACCTCAGCTGACACTGATATCCCCCATCAACTGACTGAAGGCAAGGATCTTGTCTTATACCACTTTGTAGTTTCCTAGAGTCTTACATTTTGAATTTAGTGATAACTGAAAAACTACCCCTTATTTCTTAAACTATTACACAGGTAATTACATAGAGTTTAAAAGTTTCTTAAATACATGTCATTTAAAAAGTAGTTGTTCATGAAGTGTTAAG SEQUENCE_INCLUDED_REGION=36,280 P3_COMMENT=CL is CAGCTGACACTGATATCCCC P3_COMMENT=CR is TCATGAACAACTACTTTTTAAATGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH977 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATAAGTACAGTTCAGCCTTTTCTCCTCAAATATATAATGACTTTAACATTCCTAAGAATATAGGTATTTCTGAATGATTTAAATTTGAGGAATTTTAATACATAAAATACAATGTACAAACTTTCTGCCCACTCAGATCTCTTCTCCATCATGTACTTAGTATTTCCCATTAACCTACACACTGATTTTTATGCTACTCCTTGTAGAAACAAAATTCTGGTTTGACTCAGTTTTTGTGTTTATAAACTTTTGGAATGTGTACCCCGTTTATGTGAAGAATTATGACCTATCAGTCATAG SEQUENCE_INCLUDED_REGION=36,302 P3_COMMENT=CL is CTGATAAGTACAGTTCAGCCTTT P3_COMMENT=CR is ATGACTGATAGGTCATAATTCTTCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH978 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGAGAGAAGTCAAAGCCTGGCTTCAAAGCTTCAAAGGACAGGCTCATTCCCTTGTTAGGGGCTAATGCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTG SEQUENCE_INCLUDED_REGION=36,71 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ = SEQUENCE_ID=MH979 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGTACTTGTGATGAATGTATTTGTTCTCATTGGTTCCAGAGCATTTAAAAGTGTAAGTTGATATCAGGATTTCCTTCACCTTTTGGTCACATACTTTGCCAACTGTTAGACATTTGCGTATTTTAAATCCTACAGTTTTAGCGTGTCTTGGGCCTGTCATCTTTGCTAGTACACGTTGCTGTACTTGTCTGTTAATCCCTAGATCTTCAAAGTACAAAGAATGGTTCTAATAGGTATTGTTATCACATATAAGAAAGAACATCACATAGAAGTTTAGAGTTGTAAGGACTCTAAGGATGATCTAGCCCAAACCC SEQUENCE_INCLUDED_REGION=36,315 P3_COMMENT=CL is GATGAATGTATTTGTTCTCATTGG P3_COMMENT=CR is GGTTTGGGCTAGATCATCCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH982 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGACCTTTTAAGTGAACTATCTGGCACTGTTTGAAATTGCATCAGCCAGTTGGAATAAGAATTCGCTCTCCTGTGAGACTATTCTTATACAGCATCATGTTATGTGTGTAATAAAGTCATATTTGGCCTTTGCCCCCATGTCACATCACAGAGCCCCATTCCTGATAAGAGTGTCCTTTGTTATTCATAGCGAGCCCCTGTTAACCATACCTAAGTTTGTTCTAATGTGGTAACTCACTGTAGGCACCTGGGTAG SEQUENCE_INCLUDED_REGION=36,257 P3_COMMENT=CL is ACTGTTTGAAATTGCATCAGC P3_COMMENT=CR is CCAGGTGCCTACAGTGAGTT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH986 SEQUENCE_TEMPLATE=CTTCATGCTCCAGGTCGACTCTAGAGGATCCCTGCTGTCTGGGCCCGGCAAATTGGTTTAAGTGGAATGAAATTGGTTCCTTCCCTCCCCGTGCTCATGGCTGAGGTCCAGGCCCGATTGTAATGGGAACCTCTCCTGGGCAAAGGACATAGAAAAGCTTTCCGGGGAGTCTGACCCTCTTTTTTCATATGTGAATTCTACTTNCCCCTGGGGGTCAAGGCAATAGGCTGATAAAACATANAAATGAACCTGAAGTGGGTGGGGTGAGTGTGTGTGTGGGAAGTTGGATCAGAGGAGAATGAACAATGAACAGTGGCCGAGTGTTTTTCAAGGGCCAGTTAACCATTCCCCTGTTG SEQUENCE_INCLUDED_REGION=32,324 P3_COMMENT=CL is GGCAAATTGGTTTAAGTGGA P3_COMMENT=CR is ACAGGGGAATGGTTAACTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH989 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTATAATTTTCCCACTGAGTATGGCTTTCAGTAGTTTTATTATGATGTGCCTAGGTACATTTGTTTTTATTTGTTCTGCGAATTGTTGTATTACTTTGGGAGAAATGCTCAACTATAAATATTGCTTCTGACCCTTTTCTGTGTTCCTTCTTAAAGATACAAAATAAATGTAACATTAGACCTCTCACTATGCTGTTTTTACTCTCCTCTGATTTTTTTTTCCATTATTTTTATTGCTCTGGCTTCATTTTGTAAATNTGCTTCTTGAAATTTTTCTTTTAATTCTAGAATATCTATTTGGTTCTTTTCATGGTTTCTAGTTCTCTGAAAATG SEQUENCE_INCLUDED_REGION=33,336 P3_COMMENT=CL is TCCCACTGAGTATGGCTTTC P3_COMMENT=CR is CTAGAAACCATGAAAAGAACCA P3_COMMENT=CM is TTTTATTATGATGTGCCTAGGTACA P3_COMMENT=CC is 1,1 = SEQUENCE_ID=MH991 SEQUENCE_TEMPLATE=CTTCTGCAGGACATTTGTACCATCGAGGTCAAAGGCAACAAGAAGTGACATGAGTTTCAGTCCATCTTCATGAGGTTCCAGTTAAGGCCTGTGAATTCGCAATTGTTCTTCCCGACTGCCTGTCCTGTGAACCTCACATACTCACATCAGATGCAAAAACAACCACCTTGTAGAAG SEQUENCE_INCLUDED_REGION=0,176 P3_COMMENT=CL is GAGGTCAAAGGCAACAAGAA P3_COMMENT=CR is AAGGTGGTTGTTTTTGCATC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH993 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTAACCTTTTGTTTCTTTCTTTTTCAGTACACTGCTTTAATAAATGGTAACAGTGCTTTAATAAGTCCCCTGGCCATCATCTTACTTATGATCTCTAGATGAAGACCACTGTGTGAAACTGGTATCCTAGGAGGTTGGAGTGAGCATGTATGAGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGCTTGATTTATAAACCACTGAAGCCCGATGAAGTACTAGGGTGAAAATCACCTACGTTTCCAGAGGAAAAAAATGTCACCATCAATTCCAAGAGGGACTTGTAAACCTGAAACTAACAGCCAATGGTTTAATTTTTTTAAGGTAGAT SEQUENCE_INCLUDED_REGION=33,342 P3_COMMENT=CL is TTTCTTTCTTTTTCAGTACACTGC P3_COMMENT=CR is ACCTTAAAAAAATTAAACCATTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH996 SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTATAAGTAAGACATCTAAATTAGCAGATTTCATTCTTCCAAAATTAGAAAGTCATTAAGTTACAGATTTAGTTATGGAAGGATGGATGATGGCAGGTAAGTACTTAGGACTAAGTAGGAGCCACTCCTTAAGGAATCAATGTGCTTTTCAAATGTGTCATCTCATTTTATCCTGAACAACAGAAAATATTTTCTAGGTAAAGAAATTGAGGCTTAAAGAAGTAAAGTTATCTATAANCTAATAGTTTATAGACTCATTCCTGGTCTCTCACTAGATAATTTGCTGCGTGCTCAATTTTAGTCATGCAGGGAGTACTTACTAAACTTTACTTGGTATGCATATAATCAT SEQUENCE_INCLUDED_REGION=36,351 P3_COMMENT=CL is AAGACATCTAAATTAGCAGATTTCA P3_COMMENT=CR is CCTGCATGACTAAAATTGAGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = SEQUENCE_ID=MH997 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTTATTTCTCTGGGCACTGCTTTCTTTGGGGGCAAACTTCCAGTATCACTGATACTAATATAAAAACCCTGTAAGTCTGCTTGCATTTTCAAGATTCAATATATATCCAGATTGTTTTCCCAGCAAAGAAAATTTTATTTCTCAAGATATAAAAAATAAATATTTAATTTCAGTTTCCTCAAAAGGAATATGAAATTTGTTAAAATGCAAATCCAGCTGTAACTTTTTTGGACTTGTCTTTTATTTCTTGCTTAGTAAAAGCACTATTAGTTTGGCATCATCAAATGAGTAGGGATGAG SEQUENCE_INCLUDED_REGION=35,301 P3_COMMENT=CL is CTCTTATTTCTCTGGGCACTG P3_COMMENT=CR is TCATCCCTACTCATTTGATGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ = primer3-2.3.6/test/primer_mispriming_boundary2_formatted_output0000664000077300007730000000560212231156245024171 0ustar ioanaioanaPRIMER PICKING RESULTS FOR pair_1 Using mispriming library primer_repeat_boundary2_lib Using internal oligo mishyb library primer_repeat_boundary2_lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC RIGHT PRIMER 181 20 91.74 100.00 14.00 14.00 0.00 GCGCGCGCGCGCGCCGCGCG INTERNAL OLIGO 111 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC SEQUENCE SIZE: 182 INCLUDED REGION SIZE: 182 PRODUCT SIZE: 182, PAIR ANY COMPL: 17.00, PAIR 3' COMPL: 17.00 0 GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATAT >>>>>>>>>>>>>>>>>>>> 60 ATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCG ^^^^^^^^^ 120 CGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGC ^^^^^^^^^^^ <<<<<<<<<<<<<<<<<< 180 GC << KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 83 0 0 0 0 0 0 0 0 0 0 82 0 0 1 Right 83 0 0 0 0 0 0 0 0 0 0 71 0 0 12 Intl 163 0 0 0 0 0 0 0 0 0 0 121 0 0 42 Pair Stats: considered 381, unacceptable product size 369, no internal oligo 11, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR pair_0 Using mispriming library primer_repeat_boundary2_lib Using internal oligo mishyb library primer_repeat_boundary2_lib Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 163 INCLUDED REGION SIZE: 163 0 TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA 60 TATATATATATTATATATATATATATATATATATATATATATATATATATATATATATAT 120 ATATATATATATATATATATATATATATATATATATATATATA Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 64 0 0 0 0 0 0 0 0 0 0 64 0 0 0 Right 64 0 0 0 0 0 0 0 0 0 0 0 0 0 64 Intl 144 0 0 0 0 0 0 0 0 0 0 0 0 0 144 Pair Stats: considered 32, unacceptable product size 32, ok 0 primer3 release 2.0.0 primer3-2.3.6/test/primer1_output0000664000077300007730000011171012231156245015760 0ustar ioanaioanaPRIMER_FIRST_BASE_INDEX=1 PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 P3_FILE_FLAG=1 SEQUENCE_ID=targets_test SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=22,122 PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=1,21 PRIMER_RIGHT_0=161,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 PRIMER_PAIR_1_PENALTY=4.086374 PRIMER_LEFT_1_PENALTY=1.771733 PRIMER_RIGHT_1_PENALTY=2.314641 PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_1=2,20 PRIMER_RIGHT_1=161,18 PRIMER_LEFT_1_TM=58.228 PRIMER_RIGHT_1_TM=59.685 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=10.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=12.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=160 PRIMER_PAIR_2_PENALTY=5.046339 PRIMER_LEFT_2_PENALTY=2.731698 PRIMER_RIGHT_2_PENALTY=2.314641 PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_2=1,20 PRIMER_RIGHT_2=161,18 PRIMER_LEFT_2_TM=57.268 PRIMER_RIGHT_2_TM=59.685 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY=12.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=12.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=161 = SEQUENCE_ID=SP01-02.txt SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.334195 PRIMER_LEFT_0_PENALTY=2.101896 PRIMER_RIGHT_0_PENALTY=0.232299 PRIMER_LEFT_0_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_0_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_LEFT_0=10,22 PRIMER_RIGHT_0=248,20 PRIMER_LEFT_0_TM=59.898 PRIMER_RIGHT_0_TM=60.232 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=239 PRIMER_PAIR_1_PENALTY=2.773493 PRIMER_LEFT_1_PENALTY=2.101896 PRIMER_RIGHT_1_PENALTY=0.671597 PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT PRIMER_LEFT_1=10,22 PRIMER_RIGHT_1=247,20 PRIMER_LEFT_1_TM=59.898 PRIMER_RIGHT_1_TM=59.328 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=238 PRIMER_PAIR_2_PENALTY=2.846821 PRIMER_LEFT_2_PENALTY=2.101896 PRIMER_RIGHT_2_PENALTY=0.744925 PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA PRIMER_LEFT_2=10,22 PRIMER_RIGHT_2=249,20 PRIMER_LEFT_2_TM=59.898 PRIMER_RIGHT_2_TM=60.745 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=240 PRIMER_PAIR_3_PENALTY=3.098430 PRIMER_LEFT_3_PENALTY=2.866131 PRIMER_RIGHT_3_PENALTY=0.232299 PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_LEFT_3=12,21 PRIMER_RIGHT_3=248,20 PRIMER_LEFT_3_TM=58.134 PRIMER_RIGHT_3_TM=60.232 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=237 PRIMER_PAIR_4_PENALTY=3.235559 PRIMER_LEFT_4_PENALTY=3.003259 PRIMER_RIGHT_4_PENALTY=0.232299 PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_LEFT_4=11,22 PRIMER_RIGHT_4=248,20 PRIMER_LEFT_4_TM=58.997 PRIMER_RIGHT_4_TM=60.232 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=238 = SEQUENCE_ID=SP01-03.txt SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.785028 PRIMER_LEFT_0_PENALTY=0.040772 PRIMER_RIGHT_0_PENALTY=0.744257 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_RIGHT_0_SEQUENCE=ATCCTGCTGAAGCAAATGGA PRIMER_LEFT_0=73,20 PRIMER_RIGHT_0=174,20 PRIMER_LEFT_0_TM=59.959 PRIMER_RIGHT_0_TM=60.744 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=102 PRIMER_PAIR_1_PENALTY=0.785028 PRIMER_LEFT_1_PENALTY=0.040772 PRIMER_RIGHT_1_PENALTY=0.744257 PRIMER_LEFT_1_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_RIGHT_1_SEQUENCE=GATCCTGCTGAAGCAAATGG PRIMER_LEFT_1=73,20 PRIMER_RIGHT_1=175,20 PRIMER_LEFT_1_TM=59.959 PRIMER_RIGHT_1_TM=60.744 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=103 PRIMER_PAIR_2_PENALTY=1.015937 PRIMER_LEFT_2_PENALTY=0.271680 PRIMER_RIGHT_2_PENALTY=0.744257 PRIMER_LEFT_2_SEQUENCE=CTGCAGGTTTAGAAGGGGTG PRIMER_RIGHT_2_SEQUENCE=ATCCTGCTGAAGCAAATGGA PRIMER_LEFT_2=65,20 PRIMER_RIGHT_2=174,20 PRIMER_LEFT_2_TM=59.728 PRIMER_RIGHT_2_TM=60.744 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=8.1000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=110 PRIMER_PAIR_3_PENALTY=1.015937 PRIMER_LEFT_3_PENALTY=0.271680 PRIMER_RIGHT_3_PENALTY=0.744257 PRIMER_LEFT_3_SEQUENCE=CTGCAGGTTTAGAAGGGGTG PRIMER_RIGHT_3_SEQUENCE=GATCCTGCTGAAGCAAATGG PRIMER_LEFT_3=65,20 PRIMER_RIGHT_3=175,20 PRIMER_LEFT_3_TM=59.728 PRIMER_RIGHT_3_TM=60.744 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=8.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=111 PRIMER_PAIR_4_PENALTY=1.015937 PRIMER_LEFT_4_PENALTY=0.271680 PRIMER_RIGHT_4_PENALTY=0.744257 PRIMER_LEFT_4_SEQUENCE=CACCCTGCAGGTTTAGAAGG PRIMER_RIGHT_4_SEQUENCE=ATCCTGCTGAAGCAAATGGA PRIMER_LEFT_4=61,20 PRIMER_RIGHT_4=174,20 PRIMER_LEFT_4_TM=59.728 PRIMER_RIGHT_4_TM=60.744 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=114 = SEQUENCE_ID=SP01-06.txt SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.738411 PRIMER_LEFT_0_PENALTY=4.596160 PRIMER_RIGHT_0_PENALTY=2.142251 PRIMER_LEFT_0_SEQUENCE=TATGAATCGCAATCAAATTTTTTC PRIMER_RIGHT_0_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_LEFT_0=31,24 PRIMER_RIGHT_0=130,18 PRIMER_LEFT_0_TM=59.404 PRIMER_RIGHT_0_TM=60.142 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=7.148392 PRIMER_LEFT_1_PENALTY=5.006141 PRIMER_RIGHT_1_PENALTY=2.142251 PRIMER_LEFT_1_SEQUENCE=TTATGAATCGCAATCAAATTTTTT PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_LEFT_1=30,24 PRIMER_RIGHT_1=130,18 PRIMER_LEFT_1_TM=58.994 PRIMER_RIGHT_1_TM=60.142 PRIMER_LEFT_1_GC_PERCENT=20.833 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=101 PRIMER_PAIR_2_PENALTY=7.344055 PRIMER_LEFT_2_PENALTY=5.201804 PRIMER_RIGHT_2_PENALTY=2.142251 PRIMER_LEFT_2_SEQUENCE=TATGAATCGCAATCAAATTTTTTCT PRIMER_RIGHT_2_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_LEFT_2=31,25 PRIMER_RIGHT_2=130,18 PRIMER_LEFT_2_TM=60.202 PRIMER_RIGHT_2_TM=60.142 PRIMER_LEFT_2_GC_PERCENT=24.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=7.346874 PRIMER_LEFT_3_PENALTY=5.204623 PRIMER_RIGHT_3_PENALTY=2.142251 PRIMER_LEFT_3_SEQUENCE=TATGAATCGCAATCAAATTTTTT PRIMER_RIGHT_3_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_LEFT_3=31,23 PRIMER_RIGHT_3=130,18 PRIMER_LEFT_3_TM=57.795 PRIMER_RIGHT_3_TM=60.142 PRIMER_LEFT_3_GC_PERCENT=21.739 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=7.346874 PRIMER_LEFT_4_PENALTY=5.204623 PRIMER_RIGHT_4_PENALTY=2.142251 PRIMER_LEFT_4_SEQUENCE=TTATGAATCGCAATCAAATTTTT PRIMER_RIGHT_4_SEQUENCE=GAGCGTGCTTTGCGTTTT PRIMER_LEFT_4=30,23 PRIMER_RIGHT_4=130,18 PRIMER_LEFT_4_TM=57.795 PRIMER_RIGHT_4_TM=60.142 PRIMER_LEFT_4_GC_PERCENT=21.739 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.6000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=101 = SEQUENCE_ID=SP01-15.txt SEQUENCE_TEMPLATE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=SP01-19.txt SEQUENCE_TEMPLATE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.269854 PRIMER_LEFT_0_PENALTY=0.072951 PRIMER_RIGHT_0_PENALTY=0.196903 PRIMER_LEFT_0_SEQUENCE=TATTCCTGTCCCTGTGCCTC PRIMER_RIGHT_0_SEQUENCE=CCCTCATCCAGTGCTCTTTC PRIMER_LEFT_0=49,20 PRIMER_RIGHT_0=184,20 PRIMER_LEFT_0_TM=60.073 PRIMER_RIGHT_0_TM=59.803 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=136 PRIMER_PAIR_1_PENALTY=0.269854 PRIMER_LEFT_1_PENALTY=0.072951 PRIMER_RIGHT_1_PENALTY=0.196903 PRIMER_LEFT_1_SEQUENCE=TATTCCTGTCCCTGTGCCTC PRIMER_RIGHT_1_SEQUENCE=TCCCTCATCCAGTGCTCTTT PRIMER_LEFT_1=49,20 PRIMER_RIGHT_1=185,20 PRIMER_LEFT_1_TM=60.073 PRIMER_RIGHT_1_TM=59.803 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=137 PRIMER_PAIR_2_PENALTY=0.303362 PRIMER_LEFT_2_PENALTY=0.072951 PRIMER_RIGHT_2_PENALTY=0.230410 PRIMER_LEFT_2_SEQUENCE=TATTCCTGTCCCTGTGCCTC PRIMER_RIGHT_2_SEQUENCE=CAGTGCTCTTTCTGCCAGTG PRIMER_LEFT_2=49,20 PRIMER_RIGHT_2=176,20 PRIMER_LEFT_2_TM=60.073 PRIMER_RIGHT_2_TM=59.770 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=128 PRIMER_PAIR_3_PENALTY=0.593847 PRIMER_LEFT_3_PENALTY=0.396944 PRIMER_RIGHT_3_PENALTY=0.196903 PRIMER_LEFT_3_SEQUENCE=GCCTCAGAAACCTGGCTACA PRIMER_RIGHT_3_SEQUENCE=CCCTCATCCAGTGCTCTTTC PRIMER_LEFT_3=64,20 PRIMER_RIGHT_3=184,20 PRIMER_LEFT_3_TM=60.397 PRIMER_RIGHT_3_TM=59.803 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=5.7000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=121 PRIMER_PAIR_4_PENALTY=0.593847 PRIMER_LEFT_4_PENALTY=0.396944 PRIMER_RIGHT_4_PENALTY=0.196903 PRIMER_LEFT_4_SEQUENCE=GCCTCAGAAACCTGGCTACA PRIMER_RIGHT_4_SEQUENCE=TCCCTCATCCAGTGCTCTTT PRIMER_LEFT_4=64,20 PRIMER_RIGHT_4=185,20 PRIMER_LEFT_4_TM=60.397 PRIMER_RIGHT_4_TM=59.803 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=5.7000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=122 = SEQUENCE_ID=SP01-20.txt SEQUENCE_TEMPLATE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=SP01-21.txt SEQUENCE_TEMPLATE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.103491 PRIMER_LEFT_0_PENALTY=2.224770 PRIMER_RIGHT_0_PENALTY=2.878720 PRIMER_LEFT_0_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_0_SEQUENCE=CACACACACAACACACAGTCAA PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=156,22 PRIMER_LEFT_0_TM=57.775 PRIMER_RIGHT_0_TM=59.121 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=6.00 PRIMER_PAIR_0_PRODUCT_SIZE=156 PRIMER_PAIR_1_PENALTY=5.103491 PRIMER_LEFT_1_PENALTY=2.224770 PRIMER_RIGHT_1_PENALTY=2.878720 PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_1_SEQUENCE=CAACAGACACACACACACAACA PRIMER_LEFT_1=1,20 PRIMER_RIGHT_1=165,22 PRIMER_LEFT_1_TM=57.775 PRIMER_RIGHT_1_TM=59.121 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=165 PRIMER_PAIR_2_PENALTY=5.103491 PRIMER_LEFT_2_PENALTY=2.224770 PRIMER_RIGHT_2_PENALTY=2.878720 PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_2_SEQUENCE=CACAACAGACACACACACACAA PRIMER_LEFT_2=1,20 PRIMER_RIGHT_2=167,22 PRIMER_LEFT_2_TM=57.775 PRIMER_RIGHT_2_TM=59.121 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=8.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=167 PRIMER_PAIR_3_PENALTY=5.288748 PRIMER_LEFT_3_PENALTY=2.224770 PRIMER_RIGHT_3_PENALTY=3.063978 PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_3_SEQUENCE=ACACACACACAACACACAGTCAA PRIMER_LEFT_3=1,20 PRIMER_RIGHT_3=157,23 PRIMER_LEFT_3_TM=57.775 PRIMER_RIGHT_3_TM=60.064 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=6.00 PRIMER_PAIR_3_PRODUCT_SIZE=157 PRIMER_PAIR_4_PENALTY=5.288748 PRIMER_LEFT_4_PENALTY=2.224770 PRIMER_RIGHT_4_PENALTY=3.063978 PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_4_SEQUENCE=AACAGACACACACACACAACACA PRIMER_LEFT_4=1,20 PRIMER_RIGHT_4=164,23 PRIMER_LEFT_4_TM=57.775 PRIMER_RIGHT_4_TM=60.064 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=7.00 PRIMER_PAIR_4_PRODUCT_SIZE=164 = SEQUENCE_ID=SP03-40.txt SEQUENCE_TEMPLATE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=SP04-07.txt SEQUENCE_TEMPLATE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.103348 PRIMER_LEFT_0_PENALTY=0.051674 PRIMER_RIGHT_0_PENALTY=0.051674 PRIMER_LEFT_0_SEQUENCE=CCCTACATACAGCCAAGGGA PRIMER_RIGHT_0_SEQUENCE=TTCTTTCCGTGGCTCTCCTA PRIMER_LEFT_0=22,20 PRIMER_RIGHT_0=282,20 PRIMER_LEFT_0_TM=59.948 PRIMER_RIGHT_0_TM=59.948 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=261 PRIMER_PAIR_1_PENALTY=0.103348 PRIMER_LEFT_1_PENALTY=0.051674 PRIMER_RIGHT_1_PENALTY=0.051674 PRIMER_LEFT_1_SEQUENCE=TCCCTACATACAGCCAAGGG PRIMER_RIGHT_1_SEQUENCE=TTCTTTCCGTGGCTCTCCTA PRIMER_LEFT_1=21,20 PRIMER_RIGHT_1=282,20 PRIMER_LEFT_1_TM=59.948 PRIMER_RIGHT_1_TM=59.948 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=7.2000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=262 PRIMER_PAIR_2_PENALTY=0.117997 PRIMER_LEFT_2_PENALTY=0.066323 PRIMER_RIGHT_2_PENALTY=0.051674 PRIMER_LEFT_2_SEQUENCE=CTAAGAATGTGGCCAAGGGA PRIMER_RIGHT_2_SEQUENCE=TTCTTTCCGTGGCTCTCCTA PRIMER_LEFT_2=123,20 PRIMER_RIGHT_2=282,20 PRIMER_LEFT_2_TM=60.066 PRIMER_RIGHT_2_TM=59.948 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=7.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=160 PRIMER_PAIR_3_PENALTY=0.117997 PRIMER_LEFT_3_PENALTY=0.066323 PRIMER_RIGHT_3_PENALTY=0.051674 PRIMER_LEFT_3_SEQUENCE=TCCCTAAGAATGTGGCCAAG PRIMER_RIGHT_3_SEQUENCE=TTCTTTCCGTGGCTCTCCTA PRIMER_LEFT_3=120,20 PRIMER_RIGHT_3=282,20 PRIMER_LEFT_3_TM=60.066 PRIMER_RIGHT_3_TM=59.948 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=163 PRIMER_PAIR_4_PENALTY=0.117997 PRIMER_LEFT_4_PENALTY=0.051674 PRIMER_RIGHT_4_PENALTY=0.066323 PRIMER_LEFT_4_SEQUENCE=CCCTACATACAGCCAAGGGA PRIMER_RIGHT_4_SEQUENCE=CTTGGCCACATTCTTAGGGA PRIMER_LEFT_4=22,20 PRIMER_RIGHT_4=139,20 PRIMER_LEFT_4_TM=59.948 PRIMER_RIGHT_4_TM=60.066 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=118 = SEQUENCE_ID=AGAG SEQUENCE_TEMPLATE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=long_seq SEQUENCE_TEMPLATE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.361544 PRIMER_LEFT_0_PENALTY=0.221791 PRIMER_RIGHT_0_PENALTY=0.139753 PRIMER_LEFT_0_SEQUENCE=gatcgatgcatgcatgctac PRIMER_RIGHT_0_SEQUENCE=cccagctagctagcatcgtc PRIMER_LEFT_0=446,20 PRIMER_RIGHT_0=677,20 PRIMER_LEFT_0_TM=60.222 PRIMER_RIGHT_0_TM=60.140 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=10.00 PRIMER_RIGHT_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=8.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=232 PRIMER_PAIR_1_PENALTY=0.398026 PRIMER_LEFT_1_PENALTY=0.258273 PRIMER_RIGHT_1_PENALTY=0.139753 PRIMER_LEFT_1_SEQUENCE=gatgctagctaggcgatgct PRIMER_RIGHT_1_SEQUENCE=cccagctagctagcatcgtc PRIMER_LEFT_1=409,20 PRIMER_RIGHT_1=677,20 PRIMER_LEFT_1_TM=59.742 PRIMER_RIGHT_1_TM=60.140 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.1000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=12.00 PRIMER_PAIR_1_COMPL_END=7.00 PRIMER_PAIR_1_PRODUCT_SIZE=269 PRIMER_PAIR_2_PENALTY=0.405097 PRIMER_LEFT_2_PENALTY=0.120169 PRIMER_RIGHT_2_PENALTY=0.284927 PRIMER_LEFT_2_SEQUENCE=gcgatcgatgctagctagct PRIMER_RIGHT_2_SEQUENCE=ctagctagatcgccgctacg PRIMER_LEFT_2=373,20 PRIMER_RIGHT_2=596,20 PRIMER_LEFT_2_TM=59.880 PRIMER_RIGHT_2_TM=60.285 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=10.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=8.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=7.4000 PRIMER_PAIR_2_COMPL_ANY=10.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=224 PRIMER_PAIR_3_PENALTY=0.500121 PRIMER_LEFT_3_PENALTY=0.221791 PRIMER_RIGHT_3_PENALTY=0.278329 PRIMER_LEFT_3_SEQUENCE=gatcgatgcatgcatgctac PRIMER_RIGHT_3_SEQUENCE=gtcagctagctagatcgccg PRIMER_LEFT_3=446,20 PRIMER_RIGHT_3=601,20 PRIMER_LEFT_3_TM=60.222 PRIMER_RIGHT_3_TM=60.278 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=10.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=8.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=13.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_4_PENALTY=0.506718 PRIMER_LEFT_4_PENALTY=0.221791 PRIMER_RIGHT_4_PENALTY=0.284927 PRIMER_LEFT_4_SEQUENCE=gatcgatgcatgcatgctac PRIMER_RIGHT_4_SEQUENCE=ctagctagatcgccgctacg PRIMER_LEFT_4=446,20 PRIMER_RIGHT_4=596,20 PRIMER_LEFT_4_TM=60.222 PRIMER_RIGHT_4_TM=60.285 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=10.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=8.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=7.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=151 = SEQUENCE_ID=bad_letter SEQUENCE_TEMPLATE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC PRIMER_ERROR=Unrecognized base in input sequence = SEQUENCE_ID=bad_target SEQUENCE_TEMPLATE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC SEQUENCE_TARGET=51,10 SEQUENCE_TARGET=501,50 PRIMER_ERROR=TARGET beyond end of sequence = SEQUENCE_ID=other_bad_target SEQUENCE_TEMPLATE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC SEQUENCE_TARGET=101,1000 PRIMER_ERROR=TARGET beyond end of sequence = SEQUENCE_ID=included_test SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=21,228 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.103491 PRIMER_LEFT_0_PENALTY=2.224770 PRIMER_RIGHT_0_PENALTY=2.878720 PRIMER_LEFT_0_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_0_SEQUENCE=CACACACACAACACACAGTCAA PRIMER_LEFT_0=21,20 PRIMER_RIGHT_0=176,22 PRIMER_LEFT_0_TM=57.775 PRIMER_RIGHT_0_TM=59.121 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=6.00 PRIMER_PAIR_0_PRODUCT_SIZE=156 PRIMER_PAIR_1_PENALTY=5.103491 PRIMER_LEFT_1_PENALTY=2.224770 PRIMER_RIGHT_1_PENALTY=2.878720 PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_1_SEQUENCE=CAACAGACACACACACACAACA PRIMER_LEFT_1=21,20 PRIMER_RIGHT_1=185,22 PRIMER_LEFT_1_TM=57.775 PRIMER_RIGHT_1_TM=59.121 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=165 PRIMER_PAIR_2_PENALTY=5.103491 PRIMER_LEFT_2_PENALTY=2.224770 PRIMER_RIGHT_2_PENALTY=2.878720 PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_2_SEQUENCE=CACAACAGACACACACACACAA PRIMER_LEFT_2=21,20 PRIMER_RIGHT_2=187,22 PRIMER_LEFT_2_TM=57.775 PRIMER_RIGHT_2_TM=59.121 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=8.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=167 PRIMER_PAIR_3_PENALTY=5.288748 PRIMER_LEFT_3_PENALTY=2.224770 PRIMER_RIGHT_3_PENALTY=3.063978 PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_3_SEQUENCE=ACACACACACAACACACAGTCAA PRIMER_LEFT_3=21,20 PRIMER_RIGHT_3=177,23 PRIMER_LEFT_3_TM=57.775 PRIMER_RIGHT_3_TM=60.064 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=6.00 PRIMER_PAIR_3_PRODUCT_SIZE=157 PRIMER_PAIR_4_PENALTY=5.288748 PRIMER_LEFT_4_PENALTY=2.224770 PRIMER_RIGHT_4_PENALTY=3.063978 PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_4_SEQUENCE=AACAGACACACACACACAACACA PRIMER_LEFT_4=21,20 PRIMER_RIGHT_4=184,23 PRIMER_LEFT_4_TM=57.775 PRIMER_RIGHT_4_TM=60.064 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=7.00 PRIMER_PAIR_4_PRODUCT_SIZE=164 = PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MAX_SELF_END=8 SEQUENCE_ID=included_test_w_middle SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=21,228 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.103491 PRIMER_LEFT_0_PENALTY=2.224770 PRIMER_RIGHT_0_PENALTY=2.878720 PRIMER_INTERNAL_0_PENALTY=1.680010 PRIMER_LEFT_0_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_0_SEQUENCE=CACACACACAACACACAGTCAA PRIMER_INTERNAL_0_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG PRIMER_LEFT_0=21,20 PRIMER_RIGHT_0=176,22 PRIMER_INTERNAL_0=79,21 PRIMER_LEFT_0_TM=57.775 PRIMER_RIGHT_0_TM=59.121 PRIMER_INTERNAL_0_TM=60.680 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_INTERNAL_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=1.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=6.00 PRIMER_PAIR_0_PRODUCT_SIZE=156 PRIMER_PAIR_1_PENALTY=5.103491 PRIMER_LEFT_1_PENALTY=2.224770 PRIMER_RIGHT_1_PENALTY=2.878720 PRIMER_INTERNAL_1_PENALTY=1.680010 PRIMER_LEFT_1_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_1_SEQUENCE=CAACAGACACACACACACAACA PRIMER_INTERNAL_1_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG PRIMER_LEFT_1=21,20 PRIMER_RIGHT_1=185,22 PRIMER_INTERNAL_1=79,21 PRIMER_LEFT_1_TM=57.775 PRIMER_RIGHT_1_TM=59.121 PRIMER_INTERNAL_1_TM=60.680 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_INTERNAL_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_INTERNAL_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=165 PRIMER_PAIR_2_PENALTY=5.103491 PRIMER_LEFT_2_PENALTY=2.224770 PRIMER_RIGHT_2_PENALTY=2.878720 PRIMER_INTERNAL_2_PENALTY=1.680010 PRIMER_LEFT_2_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_2_SEQUENCE=CACAACAGACACACACACACAA PRIMER_INTERNAL_2_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG PRIMER_LEFT_2=21,20 PRIMER_RIGHT_2=187,22 PRIMER_INTERNAL_2=79,21 PRIMER_LEFT_2_TM=57.775 PRIMER_RIGHT_2_TM=59.121 PRIMER_INTERNAL_2_TM=60.680 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_INTERNAL_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_INTERNAL_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=8.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=167 PRIMER_PAIR_3_PENALTY=5.288748 PRIMER_LEFT_3_PENALTY=2.224770 PRIMER_RIGHT_3_PENALTY=3.063978 PRIMER_INTERNAL_3_PENALTY=1.680010 PRIMER_LEFT_3_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_3_SEQUENCE=ACACACACACAACACACAGTCAA PRIMER_INTERNAL_3_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG PRIMER_LEFT_3=21,20 PRIMER_RIGHT_3=177,23 PRIMER_INTERNAL_3=79,21 PRIMER_LEFT_3_TM=57.775 PRIMER_RIGHT_3_TM=60.064 PRIMER_INTERNAL_3_TM=60.680 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_INTERNAL_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=1.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=6.00 PRIMER_PAIR_3_PRODUCT_SIZE=157 PRIMER_PAIR_4_PENALTY=5.288748 PRIMER_LEFT_4_PENALTY=2.224770 PRIMER_RIGHT_4_PENALTY=3.063978 PRIMER_INTERNAL_4_PENALTY=1.680010 PRIMER_LEFT_4_SEQUENCE=GAACATGTGTGGCATTGTGT PRIMER_RIGHT_4_SEQUENCE=AACAGACACACACACACAACACA PRIMER_INTERNAL_4_SEQUENCE=TGTGTCTTGTGGGTGTGTCTG PRIMER_LEFT_4=21,20 PRIMER_RIGHT_4=184,23 PRIMER_INTERNAL_4=79,21 PRIMER_LEFT_4_TM=57.775 PRIMER_RIGHT_4_TM=60.064 PRIMER_INTERNAL_4_TM=60.680 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_INTERNAL_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_INTERNAL_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=7.00 PRIMER_PAIR_4_PRODUCT_SIZE=164 = primer3-2.3.6/test/primer_syntax_output0000664000077300007730000001617612231156245017317 0ustar ioanaioanaSEQUENCE_ID=syntax01 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_EXPLAIN_FLAG=1 P3_COMMENT="should succeeed" PRIMER_LEFT_EXPLAIN=considered 25, too many Ns 10, low tm 15, ok 0 PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7 PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=syntax02 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 P3_COMMENT="should succeeed" PRIMER_LEFT_EXPLAIN=considered 25, too many Ns 10, low tm 15, ok 0 PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7 PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=syntax03 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 P3_COMMENT="should succeeed" PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0 PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7 PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=syntax04 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 P3_COMMENT="should succeeed" PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0 PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7 PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=syntax05 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 2, PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 P3_COMMENT="should cause error" PRIMER_ERROR=Illegal SEQUENCE_TARGET value: 2, = SEQUENCE_ID=syntax06 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 2 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 P3_COMMENT="should cause error" PRIMER_ERROR=Illegal SEQUENCE_TARGET value: 2 = SEQUENCE_ID=syntax07 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 0,1 P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0 PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7 PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=syntax08 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21,1 P3_COMMENT="should cause error" PRIMER_ERROR=Illegal SEQUENCE_INTERNAL_EXCLUDED_REGION value: ,1 = SEQUENCE_ID=syntax09 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION= P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0 PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7 PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=syntax10 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION= P3_COMMENT="should succeed (whitespace in int. oligo. excl. region)" PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0 PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7 PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=syntax11 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21.0 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_EXPLAIN_FLAG=1 P3_COMMENT=should fail with syntax error PRIMER_ERROR=Illegal SEQUENCE_TARGET value: .0 = SEQUENCE_ID=syntax12 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37.01,21 PRIMER_EXPLAIN_FLAG=1 P3_COMMENT=should fail with type error. PRIMER_ERROR=Illegal SEQUENCE_INTERNAL_EXCLUDED_REGION value: 37.01,21 = primer3-2.3.6/test/primer1_list_tmp/0000775000077300007730000000000012231156372016330 5ustar ioanaioanaprimer3-2.3.6/test/primer_thal_args_output0000664000077300007730000003121612231156245017725 0ustar ioanaioanaPRIMER_TASK=generic PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=4 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_INTERNAL_DNA_CONC=200 PRIMER_INTERNAL_SALT_MONOVALENT=1 PRIMER_INTERNAL_SALT_DIVALENT=3 PRIMER_INTERNAL_MAX_SIZE=32 PRIMER_INTERNAL_OPT_SIZE=30 PRIMER_INTERNAL_MIN_SIZE=28 SEQUENCE_ID=IO_sec_str_thal_args_non_def_non_primer_args_1 SEQUENCE_TEMPLATE=CACAGTTATGCACAGAGCTGCAAACAACTATACATGATATAATATTAGAATGTGTGTACTGCAAGCAACAGTTACTGCGACGTGAGGTATATGACTTTGCTTTTCGGGATTTATGCATAGTATATAGAGATGGGAATCCATATGCTGTATGTGATAAATGTTTAAAGTTTTATTCTAAAATTAGTGAGTATAGACATTATTGTTATAGTGTGTATGGAACAACATTAGAACAGCAATACAACAAACCGTTGTGTGATTTGTTAATTAGGTGTATTAACTGTCAAAAGCCACTGTGTCCTGAAGAAAAGCAAAGACATCTGGACAAAAAGCAAAGATTCCATAATATAAGGGGTCGGTGGACCGGTCGATGTATGTCTTGTTGCAGATCATCAAGAACACGTAGAGAAACCCAGCTGTAATCATGCATGGAGATACACCTACATTGCATGAATATATGTTAGATTTGCAACCAGAGACAACTGATCTCTACTGTTATGAGCAATTAAATGACAGCTCAGAGGAGGAGGATGAAATAGATGGTCCAGCTGGACAAGCAGAACCGGACAGAGCCCATTACAATATTGTAACCTTTTGTTGCAAGTGTGACTCTACGCTTCGGTTGTGCGTACAAAGCACACACGTAGACATTCGTACTTTGGAAGACCTGTTAATGGGCACACTAGGAATTGTGTGCCCCATCTGTTCTCAGAAACCATAATCTACCATGGCTGATCCTGCAGGTACCAATGGGGAAGAGGGTACGGGATGTAATGGATGGTTTTATGTAGAGGCTGTAGTGGAAAAAAAAACAGGGGATGCTATATCAGATGACGAGAACGAAAATGACAGTGATACAGGTGAAGATTTGGTAGATTTTATAGTAAATGATAATGATTATTTAACACAGGCAGAAACAGAGACAGCACATGCGTTGTTTACTGCACAGGAAGCAAAACAACATAGAGATGCAGTACAGGTTC PRIMER_LEFT_EXPLAIN=considered 8450, GC content failed 463, low tm 4051, high tm 1050, long poly-x seq 50, ok 2836 PRIMER_RIGHT_EXPLAIN=considered 8450, GC content failed 395, low tm 4046, high tm 1103, long poly-x seq 50, ok 2856 PRIMER_INTERNAL_EXPLAIN=considered 4645, GC content failed 172, low tm 106, high tm 3532, high hairpin stability 25, long poly-x seq 30, ok 780 PRIMER_PAIR_EXPLAIN=considered 75, unacceptable product size 60, no internal oligo 8, ok 7 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.202027 PRIMER_LEFT_0_PENALTY=0.036732 PRIMER_RIGHT_0_PENALTY=0.165295 PRIMER_INTERNAL_0_PENALTY=0.174524 PRIMER_LEFT_0_SEQUENCE=GTGTGCCCCATCTGTTCTCA PRIMER_RIGHT_0_SEQUENCE=CAACGCATGTGCTGTCTCTG PRIMER_INTERNAL_0_SEQUENCE=AGTAAATGATAATGATTATTTAACACAGGC PRIMER_LEFT_0=688,20 PRIMER_RIGHT_0=933,20 PRIMER_INTERNAL_0=879,30 PRIMER_LEFT_0_TM=59.963 PRIMER_RIGHT_0_TM=59.835 PRIMER_INTERNAL_0_TM=60.175 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=26.667 PRIMER_INTERNAL_0_SELF_ANY_TH=7.04 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=3.22 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=39.32 PRIMER_INTERNAL_0_HAIRPIN_TH=37.34 PRIMER_LEFT_0_END_STABILITY=3.2700 PRIMER_RIGHT_0_END_STABILITY=3.3500 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=246 PRIMER_PAIR_1_PENALTY=0.202027 PRIMER_LEFT_1_PENALTY=0.036732 PRIMER_RIGHT_1_PENALTY=0.165295 PRIMER_INTERNAL_1_PENALTY=0.174524 PRIMER_LEFT_1_SEQUENCE=TGTGTGCCCCATCTGTTCTC PRIMER_RIGHT_1_SEQUENCE=CAACGCATGTGCTGTCTCTG PRIMER_INTERNAL_1_SEQUENCE=AGTAAATGATAATGATTATTTAACACAGGC PRIMER_LEFT_1=687,20 PRIMER_RIGHT_1=933,20 PRIMER_INTERNAL_1=879,30 PRIMER_LEFT_1_TM=59.963 PRIMER_RIGHT_1_TM=59.835 PRIMER_INTERNAL_1_TM=60.175 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=26.667 PRIMER_INTERNAL_1_SELF_ANY_TH=7.04 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=3.22 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=39.32 PRIMER_INTERNAL_1_HAIRPIN_TH=37.34 PRIMER_LEFT_1_END_STABILITY=2.8700 PRIMER_RIGHT_1_END_STABILITY=3.3500 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=247 PRIMER_PAIR_2_PENALTY=0.213910 PRIMER_LEFT_2_PENALTY=0.033504 PRIMER_RIGHT_2_PENALTY=0.180406 PRIMER_INTERNAL_2_PENALTY=4.699586 PRIMER_LEFT_2_SEQUENCE=GACAAGCAGAACCGGACAGA PRIMER_RIGHT_2_SEQUENCE=CGTACCCTCTTCCCCATTGG PRIMER_INTERNAL_2_SEQUENCE=CTGTTCTCAGAAACCATAATCTACCATG PRIMER_LEFT_2=548,20 PRIMER_RIGHT_2=762,20 PRIMER_INTERNAL_2=699,28 PRIMER_LEFT_2_TM=59.966 PRIMER_RIGHT_2_TM=59.820 PRIMER_INTERNAL_2_TM=62.700 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_GC_PERCENT=39.286 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=44.46 PRIMER_LEFT_2_END_STABILITY=3.4100 PRIMER_RIGHT_2_END_STABILITY=3.1600 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=215 PRIMER_PAIR_3_PENALTY=0.213910 PRIMER_LEFT_3_PENALTY=0.033504 PRIMER_RIGHT_3_PENALTY=0.180406 PRIMER_INTERNAL_3_PENALTY=4.699586 PRIMER_LEFT_3_SEQUENCE=GACAAGCAGAACCGGACAGA PRIMER_RIGHT_3_SEQUENCE=CCGTACCCTCTTCCCCATTG PRIMER_INTERNAL_3_SEQUENCE=CTGTTCTCAGAAACCATAATCTACCATG PRIMER_LEFT_3=548,20 PRIMER_RIGHT_3=763,20 PRIMER_INTERNAL_3=699,28 PRIMER_LEFT_3_TM=59.966 PRIMER_RIGHT_3_TM=59.820 PRIMER_INTERNAL_3_TM=62.700 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_GC_PERCENT=39.286 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=44.46 PRIMER_LEFT_3_END_STABILITY=3.4100 PRIMER_RIGHT_3_END_STABILITY=2.8200 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=216 PRIMER_PAIR_4_PENALTY=0.214007 PRIMER_LEFT_4_PENALTY=0.033601 PRIMER_RIGHT_4_PENALTY=0.180406 PRIMER_INTERNAL_4_PENALTY=4.699586 PRIMER_LEFT_4_SEQUENCE=GACAGCTCAGAGGAGGAGGA PRIMER_RIGHT_4_SEQUENCE=CGTACCCTCTTCCCCATTGG PRIMER_INTERNAL_4_SEQUENCE=CTGTTCTCAGAAACCATAATCTACCATG PRIMER_LEFT_4=508,20 PRIMER_RIGHT_4=762,20 PRIMER_INTERNAL_4=699,28 PRIMER_LEFT_4_TM=60.034 PRIMER_RIGHT_4_TM=59.820 PRIMER_INTERNAL_4_TM=62.700 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_INTERNAL_4_GC_PERCENT=39.286 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=41.67 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=44.46 PRIMER_LEFT_4_END_STABILITY=3.7100 PRIMER_RIGHT_4_END_STABILITY=3.1600 PRIMER_PAIR_4_COMPL_ANY_TH=1.07 PRIMER_PAIR_4_COMPL_END_TH=0.54 PRIMER_PAIR_4_PRODUCT_SIZE=255 = PRIMER_SALT_MONOVALENT=0.0001 PRIMER_SALT_DIVALENT=2.5 PRIMER_DNA_CONC=205 PRIMER_DNTP_CONC=1.5 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_MIN_THREE_PRIME_DISTANCE=4 SEQUENCE_ID=sec_str_thal_args_def_vs_user SEQUENCE_TEMPLATE=CGCTGCTGCGCTCCAAGCTTTACCGCTTCGCCTGCTGGCTTCGCTAAATTGCGGATTGGAAAATTTTGAAATAAGAACCCCTATCGGGCTGTTATCTGATTATGGTTATTTTGACATAGTTGTATCATCTTAAAAACAAGCATACAATCTGCAAATCTTAGACAAAGCAAAACCCCCGCCAAACGCCAATCTGCACGGGGGTTTCGAGAT PRIMER_LEFT_EXPLAIN=considered 1109, GC content failed 19, low tm 484, high tm 303, high hairpin stability 28, long poly-x seq 2, ok 273 PRIMER_RIGHT_EXPLAIN=considered 706, GC content failed 2, low tm 460, high tm 48, high hairpin stability 2, long poly-x seq 65, ok 129 PRIMER_INTERNAL_EXPLAIN=considered 905, GC content failed 1, low tm 2, high tm 646, ok 256 PRIMER_PAIR_EXPLAIN=considered 1511, unacceptable product size 1505, primer in pair overlaps a primer in a better pair 773, ok 6 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.989616 PRIMER_LEFT_0_PENALTY=0.011923 PRIMER_RIGHT_0_PENALTY=1.977693 PRIMER_INTERNAL_0_PENALTY=0.071130 PRIMER_LEFT_0_SEQUENCE=GCTCCAAGCTTTACCGCTTC PRIMER_RIGHT_0_SEQUENCE=CGTGCAGATTGGCGTTTGG PRIMER_INTERNAL_0_SEQUENCE=TTTGACATAGTTGTATCATCTTAAAAACAA PRIMER_LEFT_0=9,20 PRIMER_RIGHT_0=196,19 PRIMER_INTERNAL_0=109,30 PRIMER_LEFT_0_TM=59.988 PRIMER_RIGHT_0_TM=60.978 PRIMER_INTERNAL_0_TM=59.929 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_INTERNAL_0_GC_PERCENT=23.333 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=2.57 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=45.15 PRIMER_RIGHT_0_HAIRPIN_TH=35.65 PRIMER_INTERNAL_0_HAIRPIN_TH=36.73 PRIMER_LEFT_0_END_STABILITY=3.8600 PRIMER_RIGHT_0_END_STABILITY=3.2800 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=188 PRIMER_PAIR_1_PENALTY=4.000441 PRIMER_LEFT_1_PENALTY=0.267650 PRIMER_RIGHT_1_PENALTY=3.732791 PRIMER_INTERNAL_1_PENALTY=0.071130 PRIMER_LEFT_1_SEQUENCE=TGCTGGCTTCGCTAAATTGC PRIMER_RIGHT_1_SEQUENCE=AGATTGGCGTTTGGCGGG PRIMER_INTERNAL_1_SEQUENCE=TTTGACATAGTTGTATCATCTTAAAAACAA PRIMER_LEFT_1=32,20 PRIMER_RIGHT_1=191,18 PRIMER_INTERNAL_1=109,30 PRIMER_LEFT_1_TM=60.268 PRIMER_RIGHT_1_TM=61.733 PRIMER_INTERNAL_1_TM=59.929 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_INTERNAL_1_GC_PERCENT=23.333 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=44.25 PRIMER_INTERNAL_1_HAIRPIN_TH=36.73 PRIMER_LEFT_1_END_STABILITY=3.5600 PRIMER_RIGHT_1_END_STABILITY=6.1300 PRIMER_PAIR_1_COMPL_ANY_TH=11.82 PRIMER_PAIR_1_COMPL_END_TH=7.07 PRIMER_PAIR_1_PRODUCT_SIZE=160 PRIMER_PAIR_2_PENALTY=4.722458 PRIMER_LEFT_2_PENALTY=1.255761 PRIMER_RIGHT_2_PENALTY=3.466697 PRIMER_INTERNAL_2_PENALTY=1.447512 PRIMER_LEFT_2_SEQUENCE=CTGCGCTCCAAGCTTTACC PRIMER_RIGHT_2_SEQUENCE=ACCATAATCAGATAACAGCCCGA PRIMER_INTERNAL_2_SEQUENCE=TAAATTGCGGATTGGAAAATTTTGAAATAA PRIMER_LEFT_2=5,19 PRIMER_RIGHT_2=105,23 PRIMER_INTERNAL_2=44,30 PRIMER_LEFT_2_TM=59.744 PRIMER_RIGHT_2_TM=59.533 PRIMER_INTERNAL_2_TM=61.448 PRIMER_LEFT_2_GC_PERCENT=57.895 PRIMER_RIGHT_2_GC_PERCENT=43.478 PRIMER_INTERNAL_2_GC_PERCENT=23.333 PRIMER_INTERNAL_2_SELF_ANY_TH=4.79 PRIMER_LEFT_2_SELF_ANY_TH=13.71 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=41.95 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=2.8500 PRIMER_RIGHT_2_END_STABILITY=5.1400 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=101 PRIMER_PAIR_3_PENALTY=6.437295 PRIMER_LEFT_3_PENALTY=1.557991 PRIMER_RIGHT_3_PENALTY=4.879304 PRIMER_INTERNAL_3_PENALTY=0.071130 PRIMER_LEFT_3_SEQUENCE=AAGCTTTACCGCTTCGCCT PRIMER_RIGHT_3_SEQUENCE=GGGGTTTTGCTTTGTCTAAGAT PRIMER_INTERNAL_3_SEQUENCE=TTTGACATAGTTGTATCATCTTAAAAACAA PRIMER_LEFT_3=14,19 PRIMER_RIGHT_3=175,22 PRIMER_INTERNAL_3=109,30 PRIMER_LEFT_3_TM=60.558 PRIMER_RIGHT_3_TM=57.121 PRIMER_INTERNAL_3_TM=59.929 PRIMER_LEFT_3_GC_PERCENT=52.632 PRIMER_RIGHT_3_GC_PERCENT=40.909 PRIMER_INTERNAL_3_GC_PERCENT=23.333 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.09 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=45.15 PRIMER_RIGHT_3_HAIRPIN_TH=34.77 PRIMER_INTERNAL_3_HAIRPIN_TH=36.73 PRIMER_LEFT_3_END_STABILITY=5.5200 PRIMER_RIGHT_3_END_STABILITY=2.4000 PRIMER_PAIR_3_COMPL_ANY_TH=4.68 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=162 PRIMER_PAIR_4_PENALTY=7.552603 PRIMER_LEFT_4_PENALTY=2.405360 PRIMER_RIGHT_4_PENALTY=5.147243 PRIMER_INTERNAL_4_PENALTY=0.071130 PRIMER_LEFT_4_SEQUENCE=CTTTACCGCTTCGCCTGCTG PRIMER_RIGHT_4_SEQUENCE=GGGTTTTGCTTTGTCTAAGATTTGC PRIMER_INTERNAL_4_SEQUENCE=TTTGACATAGTTGTATCATCTTAAAAACAA PRIMER_LEFT_4=17,20 PRIMER_RIGHT_4=174,25 PRIMER_INTERNAL_4=109,30 PRIMER_LEFT_4_TM=62.405 PRIMER_RIGHT_4_TM=59.853 PRIMER_INTERNAL_4_TM=59.929 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=23.333 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=34.77 PRIMER_INTERNAL_4_HAIRPIN_TH=36.73 PRIMER_LEFT_4_END_STABILITY=4.4100 PRIMER_RIGHT_4_END_STABILITY=3.6800 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=158 = primer3-2.3.6/test/primer_ok_regions2_input0000664000077300007730000010015512231156245020000 0ustar ioanaioanaSEQUENCE_ID=ok_regions_test1 SEQUENCE_TEMPLATE=GGATCAGCTGACTCGCCTGGCTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGCCGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTGTGTGGCAGCGCCATGTCCCAAAGTTTACTCAAGACTACCCAGGAACCTCTTGCCCGAGATCCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGATGAGCTGCTTCAGAAAGAGCAAAACTATTCAGATGACGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCATCTTTGACCGAAACGAAAACCACCGTGGAGCTCCTTCCCGTGAATGGAGAGTTCAGCCTGGACGATCTCCAGCCGTGGCATTCTTTTGGGGCTGACTCTGTGCCAGCCAACACAGAAAACGAAGTTGAGCCTGTTGATGCCCGCCCTGCTGCCGACCGAGGACTGACCACTCGACCAGGTTCTGGGTTGACAAATATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGACTCAGGATATGAAGTTCATCATCAAAAATTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGTGCAATCATTGGACTCATGGTGGGCGGTGTTGTCATAGCGACAGTGATCGTCATCACCTTGGTGATGCTGAAGAAGAAACAGTACACATCCATTCATCATGGTGTGGTGGAGGTTGACGCCGCTGTCACCCCAGAGGAGCGCCACCTGTCCAAGATGCAGCAGAACGGCTACGAAAATCCAACCTACAAGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCTCTGAAGTTGGACAGCAAAACCATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGGGAAGAAACAAACCCGTTTTATGATTTACTCATTATCGCCTTTTGACAGCTGTGCTGTAACACAAGTAGATGCCTGAACTTGAATTAATCCACACATCAGTAATGTATTCTATCTCTCTTTACATTTTGGTCTCTATACTACATTATTAATGGGTTTTGTGTACTGTAAAGAATTTAGCTGTATCAAACTAGTGCATGAATAGATTCTCTCCTGATTATTTATCACATAGCCCCTTAGCCAGTTGTATATTATTCTTGTGGTTTGTGACCCAATTAAGTCCTACTTTACATATGCTTTAAGAATCGATGGGGGATGCTTCATGTGAACGTGGGAGTTCAGCTGCTTCTCTTGCCTAAGTATTCCTTTCCTGATCACTATGCATTTTAAAGTTAAACATTTTTAAGTATTTCAGATGCTTTAGAGAGATTTTTTTTCCATGACTGCATTTTACTGTACAGATTGCTGCTTCTGCTATATTTGTGATATAGGAATTAAGAGGATACACACGTTTGTTTCTTCGTGCCTGTTTTATGTGCACACATTAGGCATTGAGACTTCAAGCTTTTCTTTTTTTGTCCACGTATCTTTGGGTCTTTGATAAAGAAAAGAATCCCTGTTCATTGTAAGCACTTTTACGGGGCGGGTGGGGAGGGGTGCTCTGCTGGTCTTCAATTACCAAGAATTCTCCAAAACAATTTTCTGCAGGATGATTGTACAGAATCATTGCTTATGACATGATCGCTTTCTACACTGTATTACATAAATAAATTAAATAAAATAACCCCGGGCAAGACTTTTCTTTGAAGGATGACTACAGACATTAAATAATCGAAGTAATTTTGGGTGGGGAGAAGAGGCAGATTCAATTTTCTTTAACCAGTCTGAAGTTTCATTTATGATACAAAAGAAGATGAAAATGGAAGTGGCAATATAAGGGGATGAGGAAGGCATGCCTGGACAAACCCTTCTTTTAAGATGTGTCTTCAATTTGTATAAAATGGTGTTTTCATGTAAATAAATACATTCTTGGAGGAGCAAAAAAAAAAAAAAAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-1000 PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_THREE_PRIME_DISTANCE=0 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,216,,;,,238,216 SEQUENCE_OVERLAP_JUNCTION_LIST=256 424 554 668 861 1064 1232 1289 1423 1498 1657 1786 1886 2108 2162 2263 2410 PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION=4 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=7 = SEQUENCE_ID=ok_regions_test2 SEQUENCE_TEMPLATE=GCGGAAGGAGGGAGGGGGCCGCGCTCGGCGCCCCGGCCGGGCCACTGGGCCACAGGCCACGCGGCCACGCAGTCCGAGCGGGAGCCGAGCCGGGCGGGGCGAGGGCAGCTCCGGGAAGGAACGTCCCAGGGATGGAAGTGCTTGGATGCGGTGCTGCTGGCTGCGGATGTGCGCAAGGAGATGGGATGGAGAGCCTGAGTTGGCATTCGTATAAATGACCTGCCTGGCTCCCACCATGAGTGCTGAGCTTAACGTGCCTATCGACCCCTCTGCTCCTGCCTGCCCTGAGCCCGGCCATAAGGGCATGGATTACCGGGACTGGGTCCGCCGCAGCTACCTGGAACTGGTCACCTCTAACCACCACTCGGTACAGGCCCTGTCGTGGCGGAAGCTCTACCTGAGCAGGGCCAAGCTGAAGGCCTCCAGCAGGACCTCCGCCCTCCTCTCCGGCTTTGCCATGGTGGCCATGGTGGAGGTGCAGCTGGAGACGCAGTACCAGTACCCGCGGCCGCTGCTGATTGCCTTCAGCGCCTGCACCACGGTGCTGGTGGCCGTGCACCTGTTCGCCCTCCTCATCAGCACCTGCATCCTGCCCAATGTGGAGGCCGTGAGCAACATCCACAACCTGAACTCCATCAGCGAGTCCCCGCATGAGCGCATGCACCCCTACATCGAGCTGGCCTGGGGCTTCTCCACCGTGCTTGGCATCCTACTCTTCCTGGCCGAGGTGGTGCTGCTCTGCTGGATCAAGTTCCTCCCCGTGGATGCCCGGCGCCAGCCTGGCCCCCCACCTGGCCCTGGGAGTCACACGGGCTGGCAGGCCGCCCTGGTGTCCACCATCATCATGGTGCCCGTGGGCCTCATCTTCGTGGTCTTCACCATCCACTTCTACCGCTCCCTGGTGCGCCACAAAACGGAGCGCCACAACCGCGAGATCGAGGAGCTCCACAAGCTCAAGGTCCAGCTGGACGGGCATGAGCGCAGCCTGCAGGTCTTGTGAGGGGCCGAGGGCCGGGGCTGGGAGCGGCCCTGTGCCCGGGAGTCCGCAGAGGCGGGGATTTGTCAGATGCAGACATTTTGCAAGGCTGCCGGGTAGTTCAAGACCAAAGTTTTCCTCTTGTCTTAATACCATAAGGACTGGATGACTTCTCCTGAGATAGAACCGTTTGGTTCAATGAGGGACTGTGTTGCTAAGAGCGTTGGGGGCAAAGCCAGGCTGGTTCCTTGGCCTCGGGGTTTCCTGGGTCGGGGACACGGTGAAGAGGCTCCAGCGGGACCTGCCCATCAGTCCTGGGCCAGGAGGGGCTCCAAGCAGCACCCAGCGGTCCGGGGGAGTCTCAGACCCGGCATGCGTGGCTGGCAGACCTGGGAGAGCCAGGGCAGGGTTTTGCGTTCAGAGAAGGATTGCCCCAGAGACCCGTGGTGGACTTCATGGGTGCTGAGTGGCCCGTGTGACAGTGATGACACGAAGGCTTCGGCGTTTGAGTGGGTGCAGGTGCACGCCAGGGCTTGGTGCTTCCCTGCCTGGCCCTGGAGGGAGCTGGGTGGCCTGGCTTCAGGGGAAGACAGGAGCCAGGACACACGTCAGCCCAGCAGGTGTGGGGGGTGCTGCAGCCCTCGGCAGTGGGGTCAGGCCCTGGGGGATGTTTCCAATGGTGGGCAGCCTGGCCAGGCCGGAGAAGACATGTTCACGGGCATCTATCAGATGCCCCCTTGAGGAGGCTGAGTTATTTGAGGGCTGCTGCAAAGTACGCTAGGCTCAAATTCTCTTTTCCCAGCCAGAGCCCTGGCCACACGGACTCAGAGGGGCCACCGGGGTGGGGAAAGGACCCCTCCCCCACCCCCCGCAGCCACTGGCCTCCAGCTCTCGGCCACAGAATGGCCTCTAAGGCTGACTCAGCCACTCCCTTGGGCTGTGGCAGCAGGAGGCGGGGGCTCTGGCTCAGGCCCCGGAGCCTGTGCAGCTTGCCCATGGCCCTAGGCAGCGAGGGGACAGCCTGGGGGACTTCCTGCCTAGGCAAGGTCATTGGCCGGGCCTGGCCTGTGGATAGTGGGGCCAGGGGCCGGCCCAGGCCAAATGAGTGCCCTCCTTGTTATGACACCAAGTGACTACAAGGGAGGCAAGACCCCTCCAGGCCTCTCAGCCGACACTGGGTCCCACCACACACAGTGACTGTGCCGTGCAGTGCAGGTTCTGGCCTTTTCCTTGAAGGCATCTGGTAGACCCGAAGCCACGCTCTCGGGCCGCACATGCACGCCGCAGCACCAGCTGCCCTGAGCTGCTTGTACAACCAAACACCTTTCCCCTCTTCTCCAGCTGTAACCTGGAGAGTCAGCCATGCCTTGTCTTTTGTTCTCATAAATAGTCACTGGGGCCGGGCGCAGTGACTCACGCCTGTAATCCCAGCACTTTGGGAGGCCTAGGTGGGCGGATCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAAAATACAGAAAATTAGCTGGGCGTGGTGGCGGGCGCCTGTAGCCCCAGCTACTTGGGAGGCTGAGGCGGGAGAATGGCAATGGCGTGAACCCGGGAGGCAGAGCTTGCAGTGAGCTGAGATGGCGCCACTGCACTCCAGCCTGGGCGACAGAGCCAGACTCCATCTCAAAAAAAAAAAAAAAATTAGCCGGGCGTGATGGCGGGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAAGAGAATCACTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCACGCCATTGCACTACAGCCTGGGCAACAAGAGCGAAACTTTGTCTAAAAAAAAAAAAAAATCACTGGGCTTCTTCATGCTCTGATCACATCTCTCGTAAAAGCTTAAGCTCTCTCCGGGGTCCGGGTTGGCCGTGCCGTGGAATTCTGGGTGGCCTGGCTGGGGTCTCTGGAAATGTGGCTGCAGCAGAGAACAGAGACCCTGACATGCAGTTTTCCGTGCTGAGGGGCCCTAGGGGAGTCACACCAAGGGTCCCCACGAGAAAGTTGTGGCATCCCCGGGGGCCGGAGAAGAGCCCCGTGTCTTCTGAGGGGTTCGTCCTTTGTGTCCCCTGCAGACATTTGTCTGCGACCTTTGCCCTCCAGCATGTATGTACTTTCCTGCAGCCTGTAGAAACGCCTCTTACGGTTTAATATGTGTTCGCTTTGCTAAAGAATATCAACATCGGCCAGGCGAGGTGGGGCACGCCTGTCATCCCAGCACTTTGGGAGGCTGAGGTGGGAGGATCACTTGGGCCCAGGGGTGCAAGACCAGCCTGGGCAACATAGCGAGACCCCATGTCTAAAAAAATTATTTTAAATTAGCCAGGCCGGGTGCAATGGCTCGCGCCCGTAATCTTAGCACTCTGGGAGGCCGAGGCAGGCAGATCACTTGAGATCAGGACTTTAAGACCAGCCTCGGCAACAACATGGTGAAACCATCTCTAGCAAAAATACAAAAAATTAGCCGGGTATGGTGGCGGGTACCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAAGAGAATCGCTTGAACGCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCGTGCCACTGCACTCCAGCCTGGACAACAGAGCAAAACTCTGTCTCAAAAAATAATAAATAAAAATAAATTAGCTGGGCGTGGTGGTGCATGCCTGTAGTTCCAGCTACTTGGGAGGCTGAGGTGGGAGGATTGCTTGAGCCTGGGAAGTAGAGGCTGCAGTGAACTATAACTGTGCTAGTGGCCGGGCGCAGTGGCTCACGCCTATAATCCCAGCACTTTGGGAGGCCAAAGCAGGTGGATCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACTCTGTGTCTACTAAAAATACAAAAAATTAGCTGGGTGTGGTGGCGCTCGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGAACCCAGAAGGCAGAGGTAGCAGTGAGCCAAGATGGTGCCACTGCACTCCAGCCTGGGCGACAGAGGGTGACTCCTCAAAAAAAAGAAAAATAGCTAAAATATGATTGTACCACTGCACTCCAGCCTGGGTGACAGAGTGAGACCCTGTCTCTAAAAAAAAAAAAAAGAAAATAAAGAAAAAAGAATGTCAGCATGTCAGCGTTCCTCCAAGGAGTGGCTTTGGGTCAAGAAGACAGGCTGGCTCTCCTAAACAAACGGCTCCCCACGGCTGGGAGAGGCAGCAGGCCCTTACTCTGGCCTGAGTCTTTTGGGAGGGGCCCTGCTGGGCCTTCTCCTGCCACCTACCTTCCTGGACCATCTGTGCCCCTGCCCACGCAGTACCACACTCCCCAGACTGTGGAACGCATCTCCAGTGCCATCTGGGGGCCAGTTACTGTACCTGGTCCCCAGGCAGAGCTCTTGGCCTGGCAGCATCCAGGCAGGTATTAAGTGCCCAGTGATGGTGGCACATGGGTGGGGACGGAGCCACATCCAAGTCTAGCCCTCTGATTAGGAGGATGACAGTCTTGGAATTTCTTGTTCTCTTTCTCCTAGGGTTCTCGTTCTCCCAGCTCCAGCCTTTCTAGTTTCGAGGCATGAGGCAAACCATTGCTGTGGGAATAGCAGTGACCATTGGTAGGGCCATGGTGGCCGGGAGAGGAAACCTCAGGGTCACCCCAGAGCCTGTGGGGAGACGGAGGGTGAGCTGTTGTTGGAGCTGCAGAAACGCCTTGAAGGGCAGCATGGGCGATGAGGAATGCCATTGCAGCCTAGAAATTCGGTTTCTGGAGGTTTAGCCTGATTTTGAAGAGTATTTAAGAACAGAGGGCTCCAGACCAGGTGTGGTGGCTCACATCTGTAATCCCAGCACTTTGGGAGGCAGAGGTGGGCGGATCGCCTGAGATCAGGAGTTCGAGACCAGCCTGGCCCACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTAGTGACACACGCCTGTAATCCCAGCTGCTCGGGAGGCAGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCACGCCACTGCACTCCAGCCTGGGTAACAGAGCAAGACTCTGTCTCAAAAAAAAAAAAAAAAGAACAGGGGCCTCTGCCTCAGTTTGCCCGGGAGCCAGCCAGGGCCCATCCTAATTTGGAGCACAGTCTTCCTGGTGCCTAGACATGCCAAGGCCCCTCCCACGTGGTACACCCTCTCCGTTTAGTACCTGACCACCTGTTTCAAAACGCAGGTGTTTCTGGTTTAGAAACTTGGAAGGCGGAATGTGTTTTCGTGTCTTCTAGGAAGGGTCTGCTGAGGACCAGACCACGTAAGCCTGAGTGGATCCTGACTCAGCTGCAGCCCTTACCTGCCTCGTGCTGATGATCTATGCATGGCGTTATGTAGATCACGTGCGGCAGAGACAGCCACTGTCCTGTGTGCGGGTTTTTAAAACAGCTGCCCTGGATGAAACGGAATAAACCAGTGATGCTATTCAAAGTCTTTCTGTTCTTGGGGGATGCTGTGGGCAGAGGGGATAAATTGCAGCCCCTGGCCCTCCCGTGGCATCTGCTGACTGGGGGACCCACATCGTGGCCTGAAAAACTCCTGGAGCGCCCTCTGGCCTCCTGTCGCTGGTCACTGGCCAGTGTCCTCCCAGGCTGCCCCGGCAAGGGGGCGTGGACATCTCTAGAATGGAGGCAGGGTACTCATGTCCAGACACGCCGCCATGGGGCTCCGCCCCGAGAGCGCAGGGTGCCTCTGCCTGGCGCTTCCCCTCCCAGACAGGGCCCTCTCTTTCCAGAAACAAAAGAAGGGGCAGCCCCAGAGGGTGGCTGAGCGACAACAGAGCCCACCTGGACAGGGTCAGCTTGGCGTTCCCCCCAGTCCCTTGAGGAGAGGAGCTACCAGAGCACTGGTCACCTCAGGACATTCTCCAGCCCCAAGTCCCTGTGGGGCAGCAGGAACAGGTGGGTCCCTCCTGCGGGCTGGTGGCACTGCTAAGGAAGCACCAGACAGCCTTTTTTTTTTTTTTGAGACAGAGTCGCTCTGTCACCCAAGCTGGAATGCGGTGGCATGATCTTGGCTCACTGCAACCTCCTCCTCCCAGGTTCAAGCGATCTTGTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCACGCACCACCATGCTTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCATGACCTCGTGATCTGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCAGCTTGCCCAGCTTATGTTTATAGATTTATTTTAGAGACAGGGTTGGGCTCTATTGCCCAGGCCAGAGCTGGAGTGCAGTGGTGCAATCATAGCTCTCTGCAGGCTCCAGCTTCTGGGCGCAAGCAATCCTCCCACCTCAGTCTCCCAAGTAGCTACACTTGGGAGACTACGGGCGCACACTACCATGCCCAGCTAATGTTTTTTTTGTAGAGATGGGATCTTGCTATGTTGCCCAGTCTGGTCTTGAACTTCTGGCCTCAAGCGACCCTCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGCGAGCCACCATGCCTGGCCCATTCTGTTTTTTGTCGGCCACTGAGGTAGAGGCCCTCTTCTAGGAAAGAATTTCTGTTTGTTTTGTTTTGTTTTGTTTTGTTTTTTTGAAACAGTCTCTCTCTGTCTCCCAGGCTGGAGTGCAGTGGCGTGATCTCAGCTCGCTGCAACCTCCACTTCCCAGGTTCAAGCAATTCTTCTGCCAAGCTGGGACTACAGGCATGCACCACCACCATGCCCTGCTAATTTTTGTTGTTGTCGTTGGTTTTTGAGATAGAGTTTCGCTCTTGTTGCCCAGGCTGGAGTGCAGTGGCACGATCTCAGCTCACCGCAACCTCCGCCTCCCAGGTTCAAGCAATTATCTTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCATGCGCCACCACACCTGGCTTATTTTTGTATTTTTAGTAGAGATGGGGTTTCTCCATGTTAGCCAAGCTGGTCTCGAACTCCCGACCTCAGATGATCCGCCTGCCTCAGCCTCCCAAAGTGCTGGGGTTACAGGTGTGAGCCACCACACCCAGCCTTTCTTTTTGCATTTTTAATAGAGACTGAGTTTCATCACACTGGCCAGGCTGGTTTCGAACTCCTGGCCTCAAGTGATCCGCTGGCCTCAGCCTCCTAAAGTGCTGGATTACAGGCATGAGCCACCGTGCCAGGCCTTGTTAATATTTTTAACTCTGGCACTTCACCCTAGAGGCTGCTAGGAATCAGGTGTGCCAAGCAACTGGCACCACTCCATGGAAAGCGTCTAGGGCAGTGCCTTGGTTCTGCCAATCCTGAGAAGCCAGGCTGCCCCTTGGGCTGGGCAGTGGCCTCAGTACCGAGCCCCAGGCCAGCAAGCCCACAAGCCTCGAGAAAGACTGCACTTCCGGTGGAGGCTGCAGTTTCCTTAAAGGGAAAATCTGCCCCACCCCTGTCAAGGGCAGGACACAAGTCACTGGCATTGAAGGACCCACTCCACACCCAGCAGATCATCGACGCAGACTTTTTTTTTCCCAGATAGAGTCTCACTCTGTCGTCCAGGCTGGACTGCAATGGTGTGATCTTGGCTCACTGTAACCTCCGCCTCCCGGGCTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTAGGATTACAGGCGCCCACCATCACGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTTGCCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGATCCTCCTGCCTCAGCCTCCCAAAGTGCTGGGATTATAGGCGTGAGCCACCTCGTCCGGCCCAAGACTTTTTGCCTCAGATGCTGCAGAGCCCTGGTGGGGCCCAGGAGGGCAAGGCCCACACAGGAGCCGAAACCCATGTAGAACCTGCCAACCTGTAGCTCACACCTATAATCCCAGCACTTTGGGAGGCTGAGGTGGGCGGATCGCTTGAGCCAAGGAGTTCGAGACCAGCCTGGGCAACATTACGAGACCCTGTCTCTACAAAAATACAAAAATTACCCGGGCTTGGTGGCTCGTGCCTATAGTCCCAGCTACTGGGGAGGCTGAGGTGGGAGAATCATCTGAGCCCAGGGAGGTTGAGACTGCAGTGAGCCAAGATTGTGCCACTGCACTCCAGCCTGGGTAAAATGAGTGAGACCACGTCTCAACCCCTTTCACAGCCCGTCTGCGGCCCACCCACCCATGGGTACTGTGGGATGGGGGACAGGCTGGCTTAACACAAATCGAGGCAGGAATAACCCCAGAGAATGGGCTTTGCATGGAGCTTGGCTCCTGTCCCTGCCTGTGAGGGAGGACCAGACTCGGCCTCACCACCTGCCACTCTGAGCAAACAGGCAACGGTGTTTCCTGAACATCTTTCTGAAGCGGCTGAGGGATGTCAGCTGAGCCCCCGCTGGGCCTGCTCTGGAGCGGGATGTCTCCAGAAGCCGCCCTTGGAGCGGGCACTTCCCTATTTGGGCGTGTCCCAGTCCCATGCCTCACCATCCCCTTGCTTGAAGCTCCAAGAGCATGAGAGTGGGCAGCCTGGTCTGCTGAGGAAAGTGTCTGATGGATGCGGAAATGGCCACCCCAAACACCGGTAAGCAGATGTTACCCTGCAGGCGGTGGCTCCTGGGGCCCAGCCCTGCAGAAACACATGGGGCAGGCTGGGCAGAGGGGCTCACACCCGTTATTCCCAGCGCTTTGGGAGGCTGAGGCGGGAGGATCGCTTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACATAGCAAGACTCTATCTCCACTAAAAATCAAAACAAAACAATTAGCTGGGTATGGTGGCACACGCCTGTAGTTCCAGCTACTGGGGAGGCTGAGGGGGAGGATCACTTGAGCCCAGGAGTTCAAGGCTGCAGTGAGCCATGATTGCGCCACTGCACTCCAGCCTGGGCAACAGAGCAAGACCCTGCCCCGCCGCCTGAAAGAAAGAAAAAAGAAAGAGAAAGGAAGAGAAAGAAAGGAAAAGAAAAAGGAAACATACGAGGCTATGGGGCCGTGTACGGGGGCCAGGCTGTGGCAGAAGCATCCTGGCGTGGCCTTCTCCAGCCCCATCCTGGTTGGGTAAGGTGTCGCCGTGGGTCTTCGTGTCACAGCTTCAAGGACAGACCCAACTCTGGTATCACAAACTTAACAGGTAATGGAGTTTCCTAAAGTGTGCTCTGCAGGAGTGCTGGAAGGGCCAGCATGGAAGGACCCCAGGGAGGGAGATTTCTTTGCTCCACAGCATCCCCAAGCCTCACGAGGCCTCTCCCCTCCAAGTCTCTATGTGCATCGTTCTCCAGATGTTGGAGCATCCTGCAGGGGCCAGCCCAGCCTGTCAGGGGCAGAGACCCTGCACTGGCCCCCTCGAGAGTGGACCCCTGGGCTGGCTTATCTGCTCCAGTGCAGTGAGGATCAGCTGGAGCTCCGACCTGACTCACATCTGGATGCGCAGTGAGGGGCCCGTTGGGGCCGTGATACTGGGAGGAAGCCGGGCTCTGTGCTCTTGCTCCAAGTGAGTTGACCAGGGAGCCGAAGTTAAGGTGGCCTGGGAGCCACGTCCTCCCACCTAGCGGCTGGGCTCTCACAGCCACTAGAAACCCATCAGCCTTTCCCCTGGGTCTATGCGGGGCTCTTCAGCCCTCCTGGGAGCTCAGTGGCTGGGATGCTGCTTTACAGACAGTGCACGGCTCTGCCATCTGACAGCTCCCAGAGGGTTCTGGGGGTGACTGTAAAGAGTGGTGCTGGCCCCGAACCTTTCCTGTTCCCCTGGATTTAGTTCCCAGCAGGTGGTGAGGAACTGAACATTTTGTCCCATAGTGGTGGGAGAGAGGGCTGGCCACATGGGTCCAGGCCACCCCCTCAGCCTCCACAGCCTGCCCCGCACCCAGCCACAGGAACAGCAGAGCAGCTCCCCCATCCAGGTAAGCGCCCCCACCACAGCCCCATCACAAAGGAATAGCCTCCTGGTGTAGTGGGAGGTGAGGAGCTTTTTTTGTTTTCCTTTTATTTATTTTAGGGACAGGGTCTTGCTCTGTTGCCAAGTTAAGTGCAGTGGTATGATCATGAGCTCACTGCAGCCTTGACATGGTGGTAAAGCCATCCTCCCGCCTCAGCCTTTCGAGTAGCTGGGACTACAGGTGTGTGCCACCATGCCCAGACGCCCAGCTAATGTTTGTATTTTTTGTAGAGATGGGGGTCTCACTATGTTGCCCAGGCTGGCCTCAAGCAATCCTCCCACCTGAGCCCCGCAAAGTACTCGGATTACAGGTGTGAGTCACCGCGCCTGGCCGGGAGCTTTAATTTTTCCTACTTGTTAACAGCGTTATGGGGCTGGAAGGAGGCTCTCAGATTTCCCTGCCCTCCTCACATCCCCCAACCCTCCCTGGGCGTGGGGGTGTCAAGCATGAGTCAGGCTGAGCCTGACTCTGAAGCTGTTCCCAGCTCTTTAGTCTAAACTTCAAATCTTCAACCCTGTTCCCTAATCCAACACATTCCCCAAATGAAGGGACTGGACAGTGAGATATCGGGAAACCCCATGAAAATAGATGCATAATAAAGGTGAAAATCATT PRIMER_NUM_RETURN=100 PRIMER_PRODUCT_SIZE_RANGE=75-800 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,251,,;,,1,251; PRIMER_MIN_THREE_PRIME_DISTANCE=0 = SEQUENCE_ID=ok_regions_test3 SEQUENCE_TEMPLATE=GGATCAGCTGACTCGCCTGGCTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGCCGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTGTGTGGCAGCGCCATGTCCCAAAGTTTACTCAAGACTACCCAGGAACCTCTTGCCCGAGATCCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGATGAGCTGCTTCAGAAAGAGCAAAACTATTCAGATGACGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCATCTTTGACCGAAACGAAAACCACCGTGGAGCTCCTTCCCGTGAATGGAGAGTTCAGCCTGGACGATCTCCAGCCGTGGCATTCTTTTGGGGCTGACTCTGTGCCAGCCAACACAGAAAACGAAGTTGAGCCTGTTGATGCCCGCCCTGCTGCCGACCGAGGACTGACCACTCGACCAGGTTCTGGGTTGACAAATATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGACTCAGGATATGAAGTTCATCATCAAAAATTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGTGCAATCATTGGACTCATGGTGGGCGGTGTTGTCATAGCGACAGTGATCGTCATCACCTTGGTGATGCTGAAGAAGAAACAGTACACATCCATTCATCATGGTGTGGTGGAGGTTGACGCCGCTGTCACCCCAGAGGAGCGCCACCTGTCCAAGATGCAGCAGAACGGCTACGAAAATCCAACCTACAAGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCTCTGAAGTTGGACAGCAAAACCATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGGGAAGAAACAAACCCGTTTTATGATTTACTCATTATCGCCTTTTGACAGCTGTGCTGTAACACAAGTAGATGCCTGAACTTGAATTAATCCACACATCAGTAATGTATTCTATCTCTCTTTACATTTTGGTCTCTATACTACATTATTAATGGGTTTTGTGTACTGTAAAGAATTTAGCTGTATCAAACTAGTGCATGAATAGATTCTCTCCTGATTATTTATCACATAGCCCCTTAGCCAGTTGTATATTATTCTTGTGGTTTGTGACCCAATTAAGTCCTACTTTACATATGCTTTAAGAATCGATGGGGGATGCTTCATGTGAACGTGGGAGTTCAGCTGCTTCTCTTGCCTAAGTATTCCTTTCCTGATCACTATGCATTTTAAAGTTAAACATTTTTAAGTATTTCAGATGCTTTAGAGAGATTTTTTTTCCATGACTGCATTTTACTGTACAGATTGCTGCTTCTGCTATATTTGTGATATAGGAATTAAGAGGATACACACGTTTGTTTCTTCGTGCCTGTTTTATGTGCACACATTAGGCATTGAGACTTCAAGCTTTTCTTTTTTTGTCCACGTATCTTTGGGTCTTTGATAAAGAAAAGAATCCCTGTTCATTGTAAGCACTTTTACGGGGCGGGTGGGGAGGGGTGCTCTGCTGGTCTTCAATTACCAAGAATTCTCCAAAACAATTTTCTGCAGGATGATTGTACAGAATCATTGCTTATGACATGATCGCTTTCTACACTGTATTACATAAATAAATTAAATAAAATAACCCCGGGCAAGACTTTTCTTTGAAGGATGACTACAGACATTAAATAATCGAAGTAATTTTGGGTGGGGAGAAGAGGCAGATTCAATTTTCTTTAACCAGTCTGAAGTTTCATTTATGATACAAAAGAAGATGAAAATGGAAGTGGCAATATAAGGGGATGAGGAAGGCATGCCTGGACAAACCCTTCTTTTAAGATGTGTCTTCAATTTGTATAAAATGGTGTTTTCATGTAAATAAATACATTCTTGGAGGAGCAAAAAAAAAAAAAAAA PRIMER_NUM_RETURN=900 PRIMER_PRODUCT_SIZE_RANGE=75-100 150-250 300-400 500-600 650-800 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=237,452,,;,,237,452;238,216,200,1500;0,1000,238,216 PRIMER_MIN_THREE_PRIME_DISTANCE=0 = PRIMER_MIN_THREE_PRIME_DISTANCE=-1 SEQUENCE_ID=ok_regions_test4 SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-200 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MIN_TM=0 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,, PRIMER_EXPLAIN_FLAG=1 = SEQUENCE_ID=ok_regions_test5 SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-200 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MIN_TM=0 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,400,20 PRIMER_EXPLAIN_FLAG=1 = SEQUENCE_ID=ok_regions_test6 SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-200 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,238,20 PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=20 = SEQUENCE_ID=ok_regions_test7 SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-200 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MIN_TM=0 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,400,20;238,20,, PRIMER_EXPLAIN_FLAG=1 = SEQUENCE_ID=ok_regions_test8 SEQUENCE_TEMPLATE=GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATTCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA PRIMER_NUM_RETURN=200 PRIMER_PRODUCT_SIZE_RANGE=200-200 150-182 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MIN_TM=0 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=238,20,400,20 PRIMER_EXPLAIN_FLAG=1 = primer3-2.3.6/test/primer_ok_regions_output0000664000077300007730000002312512231156245020120 0ustar ioanaioanaSEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 1 any right primer - OK PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=40-100 PRIMER_MIN_GC=0 PRIMER_MIN_TM=0 PRIMER_PICK_ANYWAY=0 SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_SIZE=16 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,20,, SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAga PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=22.403937 PRIMER_LEFT_0_PENALTY=8.942519 PRIMER_RIGHT_0_PENALTY=13.461417 PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAga PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=65,18 PRIMER_LEFT_0_TM=51.057 PRIMER_RIGHT_0_TM=48.539 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=27.778 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=65 = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 2 any left primer - OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,49,17 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=25.992387 PRIMER_LEFT_0_PENALTY=8.942519 PRIMER_RIGHT_0_PENALTY=17.049867 PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAg PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=65,17 PRIMER_LEFT_0_TM=51.057 PRIMER_RIGHT_0_TM=45.950 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=29.412 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=65 = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 3 any pair - OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,, SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccA PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=29.017881 PRIMER_LEFT_0_PENALTY=8.942519 PRIMER_RIGHT_0_PENALTY=20.075362 PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccA PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=65,16 PRIMER_LEFT_0_TM=51.057 PRIMER_RIGHT_0_TM=43.925 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=25.000 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=65 = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 4 constrained left and right primers - OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,20,49,17 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=25.992387 PRIMER_LEFT_0_PENALTY=8.942519 PRIMER_RIGHT_0_PENALTY=17.049867 PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAg PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=65,17 PRIMER_LEFT_0_TM=51.057 PRIMER_RIGHT_0_TM=45.950 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=29.412 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=65 = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 5 constrained left and right primers - NOT OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=5,10,60,10 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg PRIMER_LEFT_EXPLAIN=considered 1, not in any ok left region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 1, not in any ok right region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 6 constrained left and right primers - OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=5,10,60,7 ; 1,25,, SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=25.992387 PRIMER_LEFT_0_PENALTY=8.942519 PRIMER_RIGHT_0_PENALTY=17.049867 PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAg PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=65,17 PRIMER_LEFT_0_TM=51.057 PRIMER_RIGHT_0_TM=45.950 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=29.412 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=65 = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 7 contrained left and right primers - NOT OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,19,49,17 ; 1,20,50,16 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, not in any ok region 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 8 any left primer - NOT OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,49,16 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, not in any ok right region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 9 any right primer - NOT OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=2,20,, SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg PRIMER_LEFT_EXPLAIN=considered 1, not in any ok left region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 10 any right primer, any left primer - OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=2,20,, ; ,,49,17 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=25.992387 PRIMER_LEFT_0_PENALTY=8.942519 PRIMER_RIGHT_0_PENALTY=17.049867 PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAg PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=65,17 PRIMER_LEFT_0_TM=51.057 PRIMER_RIGHT_0_TM=45.950 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=29.412 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=65 = primer3-2.3.6/test/primer_internal1_input0000664000077300007730000002424012231156245017454 0ustar ioanaioanaPRIMER_FIRST_BASE_INDEX=1 SEQUENCE_ID=boundary_test_00 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_INTERNAL_MIN_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_INTERNAL_MAX_SELF_ANY=100 PRIMER_INTERNAL_MAX_SELF_END=100 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_INTERNAL_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-100 PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_02 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_05 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_MIN_SIZE=24 PRIMER_MAX_SIZE=24 PRIMER_OPT_SIZE=24 PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=2 PRIMER_INTERNAL_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_12 PRIMER_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_13 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_14 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=.0001 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_15 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=20 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_16 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_PRODUCT_SIZE_RANGE=50-200 PRIMER_INTERNAL_MAX_NS_ACCEPTED=5 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_17 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=20 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_18 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INTERNAL_EXCLUDED_REGION=11,50 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_19 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 SEQUENCE_INTERNAL_EXCLUDED_REGION=11,50 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNNCCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_20 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=100-300 SEQUENCE_INTERNAL_EXCLUDED_REGION=6,50 SEQUENCE_INTERNAL_EXCLUDED_REGION=61,15 SEQUENCE_INCLUDED_REGION=3,250 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATACGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT = SEQUENCE_ID=boundary_test_21 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_INTERNAL_MIN_TM=55.7 PRIMER_INTERNAL_MAX_TM=55.9 PRIMER_INTERNAL_OPT_TM=55.8 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_22 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_INTERNAL_MIN_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_INTERNAL_MAX_SELF_ANY=100 PRIMER_INTERNAL_MAX_SELF_END=100 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_INTERNAL_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_PICK_INTERNAL_OLIGO=1 P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_23 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=2,1 P3_COMMENT=should succeed with warning (internal oligo excluded region outside of included region) = SEQUENCE_ID=boundary_test_24 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=3,1 P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_25 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=1,3 P3_COMMENT=should succeed with warning (internal oligo excluded region outside of included region) = SEQUENCE_ID=boundary_test_26 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=30,1 P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_27 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=31,1 P3_COMMENT=should fail w/ no legal internal oligo = SEQUENCE_ID=boundary_test_28 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=55,1 P3_COMMENT=should fail w/ no legal internal oligo = SEQUENCE_ID=boundary_test_29 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=56,1 P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_30 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=82,1 P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_31 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=83,1 P3_COMMENT=should generate warning (external oligo excluded region outside of included region) = SEQUENCE_ID=boundary_test_32 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=82,2 P3_COMMENT=should generate warning (external oligo excluded region outside of included region) = SEQUENCE_ID=boundary_test_33 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 P3_COMMENT=should succeed (testing number of internal oligo excluded regions) = SEQUENCE_ID=boundary_test_34 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 P3_COMMENT=should fail (testing number of internal oligo excluded regions) = primer3-2.3.6/test/not_for_production_Ns.lib0000664000077300007730000001620612231156245020106 0ustar ioanaioana>ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA >MIR mammalian-wide interspersed repeat (MIR) - a consensus ACAGYAYAGCATAGTGGTTAAGAGCACGGRCTCTGGAGCCAGACTGCCTGGGTTCGAATCCCGGCTCTGCCACTTACTAGCTGTGTGACCTTGGGCAAGTTACTTAACCTCTCTGTGCCTCAGTTTCCTCATCTGTAAAATGGGGATAATAATAGTACCTACCTCATAGGGTTGTTGTGAGGATTAAATGAGTTAATAYATGTAAAGCGCTTAGAACAGTGCCTGGCACATAGTAAGCGCTCAATAAATGTTRGYTATTATT >MIR2 Repetitive element - a consensus TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT >L1 Human L1 interspersed repetitive sequence - full length copy GGCGGTGGAGCCAAGATGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGACCATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACCACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAAAA primer3-2.3.6/test/primer_first_base_index_output0000664000077300007730000002072512231156245021274 0ustar ioanaioanaSEQUENCE_ID=negative first base index SEQUENCE_TEMPLATE=TTCTCATGTTTGACAGCTTATCATCGATAAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCAATTTCTATGCGCACCCGTTCTCGGAGCACTGTCCGACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTACTTGGAGCCACTATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA PRIMER_EXPLAIN_FLAG=1 PRIMER_FIRST_BASE_INDEX=-200 PRIMER_NUM_RETURN=5 SEQUENCE_INCLUDED_REGION=-200,4361 PRIMER_PRODUCT_SIZE_RANGE=100-300 PRIMER_MIN_THREE_PRIME_DISTANCE=0 PRIMER_OPT_SIZE=20 PRIMER_MIN_SIZE=18 PRIMER_MAX_SIZE=25 PRIMER_OPT_GC_PERCENT=50 PRIMER_MIN_GC=45 PRIMER_MAX_GC=55 PRIMER_OPT_TM=60 PRIMER_MIN_TM=55 PRIMER_MAX_TM=80 PRIMER_GC_CLAMP=0 PRIMER_PAIR_MAX_DIFF_TM=40 PRIMER_MAX_POLY_X=5 PRIMER_MAX_SELF_ANY_TH=47 PRIMER_MAX_SELF_END_TH=47 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47 PRIMER_LIBERAL_BASE=0 PRIMER_MAX_NS_ACCEPTED=8 PRIMER_DNA_CONC=50 PRIMER_DNTP_CONC=0.6 PRIMER_SALT_MONOVALENT=50 PRIMER_SALT_DIVALENT=1.5 PRIMER_LEFT_EXPLAIN=considered 33850, GC content failed 23465, low tm 10, high tm 235, high end compl 5, long poly-x seq 66, ok 10069 PRIMER_RIGHT_EXPLAIN=considered 33850, GC content failed 23382, low tm 10, high tm 235, high any compl 1, high end compl 6, long poly-x seq 62, ok 10154 PRIMER_PAIR_EXPLAIN=considered 390, unacceptable product size 384, primer in pair overlaps a primer in a better pair 59, ok 6 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.112355 PRIMER_LEFT_0_PENALTY=0.109144 PRIMER_RIGHT_0_PENALTY=0.003211 PRIMER_LEFT_0_SEQUENCE=ACGCTGTAGGTATCTCAGTT PRIMER_RIGHT_0_SEQUENCE=CCTTCTAGTGTAGCCGTAGT PRIMER_LEFT_0=2534,20 PRIMER_RIGHT_0=2774,20 PRIMER_LEFT_0_TM=60.109 PRIMER_RIGHT_0_TM=59.997 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=241 PRIMER_PAIR_1_PENALTY=0.233305 PRIMER_LEFT_1_PENALTY=0.141811 PRIMER_RIGHT_1_PENALTY=0.091494 PRIMER_LEFT_1_SEQUENCE=AACTTGGTCTGACAGTTACC PRIMER_RIGHT_1_SEQUENCE=CTACTTACTCTAGCTTCCCG PRIMER_LEFT_1=3077,20 PRIMER_RIGHT_1=3367,20 PRIMER_LEFT_1_TM=60.142 PRIMER_RIGHT_1_TM=59.909 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=11.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=291 PRIMER_PAIR_2_PENALTY=0.354043 PRIMER_LEFT_2_PENALTY=0.278851 PRIMER_RIGHT_2_PENALTY=0.075193 PRIMER_LEFT_2_SEQUENCE=AGATAACTACGATACGGGAG PRIMER_RIGHT_2_SEQUENCE=AACTGGCGAACTACTTACTC PRIMER_LEFT_2=3175,20 PRIMER_RIGHT_2=3377,20 PRIMER_LEFT_2_TM=59.721 PRIMER_RIGHT_2_TM=59.925 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=5.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=203 PRIMER_PAIR_3_PENALTY=0.447657 PRIMER_LEFT_3_PENALTY=0.338513 PRIMER_RIGHT_3_PENALTY=0.109144 PRIMER_LEFT_3_SEQUENCE=GACTATAAAGATACCAGGCG PRIMER_RIGHT_3_SEQUENCE=AACTGAGATACCTACAGCGT PRIMER_LEFT_3=2407,20 PRIMER_RIGHT_3=2553,20 PRIMER_LEFT_3_TM=60.339 PRIMER_RIGHT_3_TM=60.109 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=11.4000 PRIMER_RIGHT_3_END_STABILITY=9.6000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=147 PRIMER_PAIR_4_PENALTY=0.568186 PRIMER_LEFT_4_PENALTY=0.163737 PRIMER_RIGHT_4_PENALTY=0.404449 PRIMER_LEFT_4_SEQUENCE=CTTATCCGGTAACTATCGTC PRIMER_RIGHT_4_SEQUENCE=ACTGTCCTTCTAGTGTAGCC PRIMER_LEFT_4=2621,20 PRIMER_RIGHT_4=2779,20 PRIMER_LEFT_4_TM=60.164 PRIMER_RIGHT_4_TM=59.596 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=159 = primer3-2.3.6/test/cmd_test4_output0000664000077300007730000000004112231156245016261 0ustar ioanaioanaError opening file invalid_input primer3-2.3.6/test/primer1_th_input0000664000077300007730000001315612231156245016257 0ustar ioanaioanaPRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_PARAMETERS_PATH=../primer3_config/ PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 P3_COMMENT=The next line to reproduce behavior with -default_version=1 PRIMER_DNTP_CONC=0.0 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 P3_FILE_FLAG=1 SEQUENCE_ID=targets_test SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=22,122 = SEQUENCE_ID=SP01-02.txt SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG = SEQUENCE_ID=SP01-03.txt SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC = SEQUENCE_ID=SP01-06.txt SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC = SEQUENCE_ID=SP01-15.txt SEQUENCE_TEMPLATE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC = SEQUENCE_ID=SP01-19.txt SEQUENCE_TEMPLATE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC = SEQUENCE_ID=SP01-20.txt SEQUENCE_TEMPLATE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC = SEQUENCE_ID=SP01-21.txt SEQUENCE_TEMPLATE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC = SEQUENCE_ID=SP03-40.txt SEQUENCE_TEMPLATE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC = SEQUENCE_ID=SP04-07.txt SEQUENCE_TEMPLATE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA = SEQUENCE_ID=AGAG SEQUENCE_TEMPLATE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA = SEQUENCE_ID=long_seq SEQUENCE_TEMPLATE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg = SEQUENCE_ID=bad_letter SEQUENCE_TEMPLATE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC = SEQUENCE_ID=bad_target SEQUENCE_TEMPLATE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC SEQUENCE_TARGET=51,10 SEQUENCE_TARGET=501,50 = SEQUENCE_ID=other_bad_target SEQUENCE_TEMPLATE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC SEQUENCE_TARGET=101,1000 = SEQUENCE_ID=included_test SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=21,228 = PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MAX_SELF_END=8 SEQUENCE_ID=included_test_w_middle SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=21,228 = primer3-2.3.6/test/dpal_long_input0000664000077300007730000005752712231156245016156 0ustar ioanaioana-m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTACGTAGGGCTAGCTAG l -m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTACGTAGGGCTAGCTAG e -m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATAT l -m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATATTA e GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATATT l GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATATT e GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATATT g ACGTGTTCGTCGTAAATAACATGCTATATT GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC g -m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTACGTAGGGCTAGCTAG L -m 1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATAT L GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC ACGTGTTCGTCGTAAATAACATGCTATATT L ACGTGTTCGTCGTAAATAACATGCTATATT GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC L primer3-2.3.6/test/primer_syntax_input0000664000077300007730000001074612231156245017113 0ustar ioanaioanaSEQUENCE_ID=syntax01 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_EXPLAIN_FLAG=1 P3_COMMENT="should succeeed" = SEQUENCE_ID=syntax02 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 P3_COMMENT="should succeeed" = SEQUENCE_ID=syntax03 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 P3_COMMENT="should succeeed" = SEQUENCE_ID=syntax04 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 P3_COMMENT="should succeeed" = SEQUENCE_ID=syntax05 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 2, PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 P3_COMMENT="should cause error" = SEQUENCE_ID=syntax06 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 2 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 P3_COMMENT="should cause error" = SEQUENCE_ID=syntax07 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 0,1 P3_COMMENT="should succeed" = SEQUENCE_ID=syntax08 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21,1 P3_COMMENT="should cause error" = SEQUENCE_ID=syntax09 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION= P3_COMMENT="should succeed" = SEQUENCE_ID=syntax10 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 3,5 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION= P3_COMMENT="should succeed (whitespace in int. oligo. excl. region)" = SEQUENCE_ID=syntax11 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21.0 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_EXPLAIN_FLAG=1 P3_COMMENT=should fail with syntax error = SEQUENCE_ID=syntax12 SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37.01,21 PRIMER_EXPLAIN_FLAG=1 P3_COMMENT=should fail with type error. = primer3-2.3.6/test/p3-tmpl-mispriming_input0000664000077300007730000003262012231156245017652 0ustar ioanaioanaSEQUENCE_ID=template_mis_1 PRIMER_NUM_RETURN=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG = SEQUENCE_ID=template_mis_2 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTG = SEQUENCE_ID=template_mis_3 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCC = SEQUENCE_ID=template_mis_4 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACSCCCCCCCCCCCCCCCCA = SEQUENCE_ID=template_mis_5 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACTCCCCCCCCCCCCCCCCA = SEQUENCE_ID=template_mis_6 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 PRIMER_PRODUCT_SIZE_RANGE=36-300 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCACCCCCCCCACCCCCCCCCCCCCCCCCC = SEQUENCE_ID=template_mis_7 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 PRIMER_PRODUCT_SIZE_RANGE=36-300 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC = SEQUENCE_ID=template_mis_8 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=11 PRIMER_MAX_POLY_X=40 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 PRIMER_PRODUCT_SIZE_RANGE=36-300 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC P3_COMMENT=Should succeed = SEQUENCE_ID=template_mis_9 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=9 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 PRIMER_PRODUCT_SIZE_RANGE=36-300 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC P3_COMMENT=Should succeed = SEQUENCE_ID=template_mis_10 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=6.7 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 PRIMER_PRODUCT_SIZE_RANGE=36-300 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC P3_COMMENT=Should fail = SEQUENCE_ID=template_mis_11 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_left_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_TEMPLATE=aTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGGc P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo = SEQUENCE_ID=template_mis_12 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_left_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_INCLUDED_REGION=25,26 SEQUENCE_TEMPLATE=aTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGGc P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo plus former error in ignoring included_region offset = SEQUENCE_ID=template_mis_13 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_left_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_TEMPLATE=cTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGc P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo = SEQUENCE_ID=template_mis_14 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_right_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_TEMPLATE=GCCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAT P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_11 = SEQUENCE_ID=template_mis_15 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_right_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_TEMPLATE=GCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAG P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_13 = SEQUENCE_ID=template_mis_16 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_right_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_INCLUDED_REGION=24,26 SEQUENCE_TEMPLATE=GCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAG P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_13 = SEQUENCE_ID=short_align_target_1 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_WT_SELF_ANY=0.0 PRIMER_WT_SELF_END=0.0 PRIMER_WT_NUM_NS=0.0 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_WT_END_QUAL=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_INTERNAL_MISHYB_LIBRARY= PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_INTERNAL_SALT_DIVALENT=0.0 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_INTERNAL_WT_TM_LT=1.0 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_TASK=pick_right_only PRIMER_FIRST_BASE_INDEX=1 PRIMER_PICK_ANYWAY=1 P3_COMMENT=Test for very short target of alignment number 1 = SEQUENCE_ID=short_align_target_2 PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY= SEQUENCE_TEMPLATE=ACCAAGAAAGTAGTTGACAAAAAAATGAAAACCACTATTATTTAAATTACGGAAAAAAAAAAACCAATAAACAAAAAACATAATAAAAGAAATGAAAAAAAAGAATATTAGTAGAGCATACTGAATTGTTTTCAGAAAGAGAGTGAAAGGTAACAAAAATTTACCTACTTTTCCTTTTTATTTCTCTGGTTTCGTTTCTTTTTTTTCGGCCACCCTATTTATTTTTTCACTGCTTGTAAACAACGTCTCACTTCAATAGTGTCTCCTTCATGTACTGAGATACTGCTGCAAGCGAAATAT PRIMER_PRODUCT_SIZE_RANGE=150-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=24.00 PRIMER_MAX_TEMPLATE_MISPRIMING=12.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=24.00 PRIMER_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_MAX_SIZE=27 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_MAX_TM=63.0 PRIMER_PAIR_MAX_DIFF_TM=100.0 PRIMER_TM_FORMULA=0 PRIMER_MIN_GC=20.0 PRIMER_MAX_GC=80.0 PRIMER_MAX_SELF_ANY=8.00 PRIMER_MAX_SELF_END=3.00 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_POLY_X=5 PRIMER_OUTSIDE_PENALTY=0 PRIMER_GC_CLAMP=0 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_CORRECTIONS=0 PRIMER_SALT_DIVALENT=0.0 PRIMER_DNTP_CONC=0.0 PRIMER_DNA_CONC=50.0 PRIMER_LIBERAL_BASE=1 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_MIN_QUALITY=0 PRIMER_MIN_END_QUALITY=0 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_QUALITY_RANGE_MAX=100 PRIMER_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_WT_SELF_ANY=0.0 PRIMER_WT_SELF_END=0.0 PRIMER_WT_NUM_NS=0.0 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_WT_END_QUAL=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_INTERNAL_MISHYB_LIBRARY= PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_INTERNAL_SALT_DIVALENT=0.0 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_INTERNAL_WT_TM_LT=1.0 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_TASK=pick_left_only PRIMER_FIRST_BASE_INDEX=1 PRIMER_PICK_ANYWAY=1 P3_COMMENT=Test for very short target of alignment number 1 = primer3-2.3.6/test/not_for_production_Xs.lib0000664000077300007730000001620612231156245020120 0ustar ioanaioana>ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA >MIR mammalian-wide interspersed repeat (MIR) - a consensus ACAGYAYAGCATAGTGGTTAAGAGCACGGRCTCTGGAGCCAGACTGCCTGGGTTCGAATCCCGGCTCTGCCACTTACTAGCTGTGTGACCTTGGGCAAGTTACTTAACCTCTCTGTGCCTCAGTTTCCTCATCTGTAAAATGGGGATAATAATAGTACCTACCTCATAGGGTTGTTGTGAGGATTAAATGAGTTAATAYATGTAAAGCGCTTAGAACAGTGCCTGGCACATAGTAAGCGCTCAATAAATGTTRGYTATTATT >MIR2 Repetitive element - a consensus TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT >L1 Human L1 interspersed repetitive sequence - full length copy GGCGGTGGAGCCAAGATGACXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGACCATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACCACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAAAA primer3-2.3.6/test/primer_ok_regions_input0000664000077300007730000000716512231156245017725 0ustar ioanaioanaSEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 1 any right primer - OK PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=40-100 PRIMER_MIN_GC=0 PRIMER_MIN_TM=0 PRIMER_PICK_ANYWAY=0 SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_SIZE=16 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,20,, SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAga = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 2 any left primer - OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,49,17 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 3 any pair - OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,, SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccA = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 4 constrained left and right primers - OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,20,49,17 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 5 constrained left and right primers - NOT OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=5,10,60,10 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 6 constrained left and right primers - OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=5,10,60,7 ; 1,25,, SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 7 contrained left and right primers - NOT OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,19,49,17 ; 1,20,50,16 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 8 any left primer - NOT OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=,,49,16 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 9 any right primer - NOT OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=2,20,, SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg = SEQUENCE_ID=SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 10 any right primer, any left primer - OK SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=2,20,, ; ,,49,17 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg = primer3-2.3.6/test/primer_mispriming_boundary2_output0000664000077300007730000000603412231156245022124 0ustar ioanaioanaSEQUENCE_ID=pair_1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY=primer_repeat_boundary2_lib PRIMER_INTERNAL_MISHYB_LIBRARY=primer_repeat_boundary2_lib SEQUENCE_TEMPLATE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_LIBRARY_MISPRIMING=1 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=1 PRIMER_MAX_SELF_ANY=25 PRIMER_MAX_SELF_END=25 PRIMER_PAIR_MAX_COMPL_ANY=25 PRIMER_PAIR_MAX_COMPL_END=25 PRIMER_INTERNAL_MAX_SELF_ANY=25 PRIMER_INTERNAL_MAX_SELF_END=25 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_SIZE=20 PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_INTERNAL_MAX_GC=100 PRIMER_MAX_SIZE=20 PRIMER_MIN_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MAX_TM=100 PRIMER_MIN_TM=0 PRIMER_OPT_TM=60 PRIMER_LEFT_EXPLAIN=considered 83, high repeat similarity 82, ok 1 PRIMER_RIGHT_EXPLAIN=considered 83, high repeat similarity 71, ok 12 PRIMER_INTERNAL_EXPLAIN=considered 163, high repeat similarity 121, ok 42 PRIMER_PAIR_EXPLAIN=considered 381, unacceptable product size 369, no internal oligo 11, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=63.584014 PRIMER_LEFT_0_PENALTY=31.846382 PRIMER_RIGHT_0_PENALTY=31.737633 PRIMER_INTERNAL_0_PENALTY=31.846382 PRIMER_LEFT_0_SEQUENCE=GCGCGCGCGCGCGCGCGCGC PRIMER_RIGHT_0_SEQUENCE=GCGCGCGCGCGCGCCGCGCG PRIMER_INTERNAL_0_SEQUENCE=GCGCGCGCGCGCGCGCGCGC PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=181,20 PRIMER_INTERNAL_0=111,20 PRIMER_LEFT_0_TM=91.846 PRIMER_RIGHT_0_TM=91.738 PRIMER_INTERNAL_0_TM=91.846 PRIMER_LEFT_0_GC_PERCENT=100.000 PRIMER_RIGHT_0_GC_PERCENT=100.000 PRIMER_INTERNAL_0_GC_PERCENT=100.000 PRIMER_LEFT_0_SELF_ANY=20.00 PRIMER_RIGHT_0_SELF_ANY=14.00 PRIMER_INTERNAL_0_SELF_ANY=20.00 PRIMER_LEFT_0_SELF_END=20.00 PRIMER_RIGHT_0_SELF_END=14.00 PRIMER_INTERNAL_0_SELF_END=20.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=0.00, test_1 * 2.0 PRIMER_RIGHT_0_LIBRARY_MISPRIMING=0.00, test_1 * 2.0 PRIMER_PAIR_0_LIBRARY_MISPRIMING=0.00, test_1 * 2.0 PRIMER_INTERNAL_0_LIBRARY_MISHYB=0.00, test_1 * 2.0 PRIMER_LEFT_0_END_STABILITY=13.4000 PRIMER_RIGHT_0_END_STABILITY=13.4000 PRIMER_PAIR_0_COMPL_ANY=17.00 PRIMER_PAIR_0_COMPL_END=17.00 PRIMER_PAIR_0_PRODUCT_SIZE=182 = SEQUENCE_ID=pair_0 P3_COMMENT="Should fail because of high repeat similarity" PRIMER_MAX_LIBRARY_MISPRIMING=20 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=20 SEQUENCE_TEMPLATE=TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA PRIMER_LEFT_EXPLAIN=considered 64, high repeat similarity 64, ok 0 PRIMER_RIGHT_EXPLAIN=considered 64, ok 64 PRIMER_INTERNAL_EXPLAIN=considered 144, ok 144 PRIMER_PAIR_EXPLAIN=considered 32, unacceptable product size 32, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = primer3-2.3.6/test/primer_task_formatted_output0000664000077300007730000003001412231156245020763 0ustar ioanaioanaPRIMER PICKING RESULTS FOR pcr_primers No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 1 20 58.23 50.00 10.00 0.00 CCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC PRODUCT SIZE: 160, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 2 LEFT PRIMER 0 20 57.27 45.00 12.00 2.00 TCCTACAGCTGTGGGAAAAT RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 0 0 0 0 0 0 7 0 0 0 0 0 3 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 3, ok 3 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR pcr_primers_and_io No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 9 22 59.90 40.91 6.00 4.00 TGTTTCACACTTACGGAAATGG RIGHT PRIMER 247 20 60.23 50.00 3.00 0.00 TCCTGTTGGAGAAAAGCCAC INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC SEQUENCE SIZE: 300 INCLUDED REGION SIZE: 300 PRODUCT SIZE: 239, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 0 NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTAT >>>>>>>>>>>>>>>>>>>>>> 60 CCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGC 120 CTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTC ^^^^^^^^^^^^^^^^^^ 180 TGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTC <<<<<<<<<<<< 240 CAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG <<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 9 22 59.90 40.91 6.00 4.00 TGTTTCACACTTACGGAAATGG RIGHT PRIMER 246 20 59.33 50.00 3.00 1.00 CCTGTTGGAGAAAAGCCACT INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 2 LEFT PRIMER 9 22 59.90 40.91 6.00 4.00 TGTTTCACACTTACGGAAATGG RIGHT PRIMER 248 20 60.74 45.00 3.00 0.00 TTCCTGTTGGAGAAAAGCCA INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC PRODUCT SIZE: 240, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 3 LEFT PRIMER 11 21 58.13 38.10 6.00 6.00 TTTCACACTTACGGAAATGGA RIGHT PRIMER 247 20 60.23 50.00 3.00 0.00 TCCTGTTGGAGAAAAGCCAC INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC PRODUCT SIZE: 237, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 4 LEFT PRIMER 10 22 59.00 40.91 6.00 6.00 GTTTCACACTTACGGAAATGGA RIGHT PRIMER 247 20 60.23 50.00 3.00 0.00 TCCTGTTGGAGAAAAGCCAC INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 281 210 0 0 0 0 0 30 29 0 0 0 0 12 Right 284 210 0 0 0 0 0 7 44 0 0 0 0 23 Intl 393 280 0 0 0 0 0 30 46 0 0 3 0 34 Pair Stats: considered 5, ok 5 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR left_only No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 72 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG SEQUENCE SIZE: 175 INCLUDED REGION SIZE: 175 TARGETS (start, len)*: 100,20 0 GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCA 60 CACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGG >>>>>>>>>>>>>>>>>>>> ******************** 120 GACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC KEYS (in order of precedence): ****** target >>>>>> left primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT_PRIMER 64 20 59.73 55.00 6.00 0.00 CTGCAGGTTTAGAAGGGGTG 2 LEFT_PRIMER 60 20 59.73 55.00 8.00 0.00 CACCCTGCAGGTTTAGAAGG 3 LEFT_PRIMER 56 20 60.31 55.00 8.00 6.00 AGCACACCCTGCAGGTTTAG 4 LEFT_PRIMER 65 20 59.59 50.00 4.00 0.00 TGCAGGTTTAGAAGGGGTGT Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1121 38 23 0 0 0 0 42 866 0 0 0 0 152 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR right_only No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq RIGHT_PRIMER 125 20 60.29 45.00 3.00 1.00 GTGCTTTGCGTTTTGGAAGT SEQUENCE SIZE: 136 INCLUDED REGION SIZE: 136 TARGETS (start, len)*: 90,10 0 GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGAC 60 CTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCA ********** <<<<<<<<<<<<<< 120 AAGCACGCTCCCGATC <<<<<< KEYS (in order of precedence): ****** target <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 RIGHT_PRIMER 75 20 59.27 45.00 2.00 1.00 TTGGCGTTTCTATCAGGTCA 2 RIGHT_PRIMER 124 20 60.78 40.00 3.00 3.00 TGCTTTGCGTTTTGGAAGTT 3 RIGHT_PRIMER 76 21 60.13 47.62 2.00 1.00 GTTGGCGTTTCTATCAGGTCA 4 RIGHT_PRIMER 79 21 60.26 47.62 3.00 0.00 TGAGTTGGCGTTTCTATCAGG Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Right 623 27 36 0 0 5 0 230 145 0 6 0 0 174 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR hyb_probe_only No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq INTERNAL_OLIGO 64 20 59.73 55.00 6.00 0.00 CTGCAGGTTTAGAAGGGGTG SEQUENCE SIZE: 175 INCLUDED REGION SIZE: 175 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 90,20 0 GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCA 60 CACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGG ^^^^^^^^^^^^^^^^^^^^ xxxxxxxxxxxxxxxxxxxx 120 GACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC KEYS (in order of precedence): xxxxxx excluded region for internal oligo ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 INTERNAL_OLIGO 60 20 59.73 55.00 8.00 0.00 CACCCTGCAGGTTTAGAAGG 2 INTERNAL_OLIGO 56 20 60.31 55.00 8.00 6.00 AGCACACCCTGCAGGTTTAG 3 INTERNAL_OLIGO 1 20 60.38 55.00 4.00 0.00 CATCTGCACCCTGTAAACCC 4 INTERNAL_OLIGO 65 20 59.59 50.00 4.00 0.00 TGCAGGTTTAGAAGGGGTGT Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Intl 1040 27 0 33 0 0 0 44 804 0 0 0 0 132 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR left_only No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG SEQUENCE SIZE: 20 INCLUDED REGION SIZE: 20 0 TTAGAAGGGGTGTCTGGGTG >>>>>>>>>>>>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR right_only No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG SEQUENCE SIZE: 20 INCLUDED REGION SIZE: 20 0 TTAGAAGGGGTGTCTGGGTG >>>>>>>>>>>>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR hyb_probe_only No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG SEQUENCE SIZE: 20 INCLUDED REGION SIZE: 20 0 TTAGAAGGGGTGTCTGGGTG >>>>>>>>>>>>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 primer3-2.3.6/test/primer_high_gc_load_set_output0000664000077300007730000001205212231156245021220 0ustar ioanaioanaP3_SETTINGS_FILE_USED=../test/primer_high_gc_load_set.set P3_FILE_TYPE=settings P3_FILE_ID=Test with higher Tm PRIMER_MIN_GC=62 PRIMER_OPT_GC_PERCENT=75 PRIMER_MAX_GC=80 PRIMER_WT_GC_PERCENT_GT=1.0 PRIMER_WT_GC_PERCENT_LT=1.0 P3_SETTINGS_FILE_END= SEQUENCE_ID=Load qPCR settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_TASK=pick_detection_primers PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=28.724193 PRIMER_LEFT_0_PENALTY=15.801623 PRIMER_RIGHT_0_PENALTY=12.922570 PRIMER_LEFT_0_SEQUENCE=cggactttggtggagaggg PRIMER_RIGHT_0_SEQUENCE=agctcaccggaactcccc PRIMER_LEFT_0=1221,19 PRIMER_RIGHT_0=1362,18 PRIMER_LEFT_0_TM=62.960 PRIMER_RIGHT_0_TM=62.589 PRIMER_LEFT_0_GC_PERCENT=63.158 PRIMER_RIGHT_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=10.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=142 PRIMER_PAIR_1_PENALTY=28.724193 PRIMER_LEFT_1_PENALTY=15.801623 PRIMER_RIGHT_1_PENALTY=12.922570 PRIMER_LEFT_1_SEQUENCE=ccggactttggtggagagg PRIMER_RIGHT_1_SEQUENCE=gctcaccggaactcccct PRIMER_LEFT_1=1220,19 PRIMER_RIGHT_1=1361,18 PRIMER_LEFT_1_TM=62.960 PRIMER_RIGHT_1_TM=62.589 PRIMER_LEFT_1_GC_PERCENT=63.158 PRIMER_RIGHT_1_GC_PERCENT=66.667 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=10.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=142 PRIMER_PAIR_2_PENALTY=28.724193 PRIMER_LEFT_2_PENALTY=15.801623 PRIMER_RIGHT_2_PENALTY=12.922570 PRIMER_LEFT_2_SEQUENCE=ccggactttggtggagagg PRIMER_RIGHT_2_SEQUENCE=agctcaccggaactcccc PRIMER_LEFT_2=1220,19 PRIMER_RIGHT_2=1362,18 PRIMER_LEFT_2_TM=62.960 PRIMER_RIGHT_2_TM=62.589 PRIMER_LEFT_2_GC_PERCENT=63.158 PRIMER_RIGHT_2_GC_PERCENT=66.667 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=10.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=143 PRIMER_PAIR_3_PENALTY=28.724193 PRIMER_LEFT_3_PENALTY=15.801623 PRIMER_RIGHT_3_PENALTY=12.922570 PRIMER_LEFT_3_SEQUENCE=cccggactttggtggagag PRIMER_RIGHT_3_SEQUENCE=gctcaccggaactcccct PRIMER_LEFT_3=1219,19 PRIMER_RIGHT_3=1361,18 PRIMER_LEFT_3_TM=62.960 PRIMER_RIGHT_3_TM=62.589 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=66.667 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=10.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=143 PRIMER_PAIR_4_PENALTY=31.141401 PRIMER_LEFT_4_PENALTY=15.801623 PRIMER_RIGHT_4_PENALTY=15.339778 PRIMER_LEFT_4_SEQUENCE=cggactttggtggagaggg PRIMER_RIGHT_4_SEQUENCE=gctcaccggaactccccta PRIMER_LEFT_4=1221,19 PRIMER_RIGHT_4=1361,19 PRIMER_LEFT_4_TM=62.960 PRIMER_RIGHT_4_TM=62.498 PRIMER_LEFT_4_GC_PERCENT=63.158 PRIMER_RIGHT_4_GC_PERCENT=63.158 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=8.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=141 = primer3-2.3.6/test/primer_global_err/0000775000077300007730000000000012231156372016524 5ustar ioanaioanaprimer3-2.3.6/test/primer_global_err/bad_settings0.out0000664000077300007730000000011412231156245021776 0ustar ioanaioanaPRIMER_ERROR=Settings file is empty: ./primer_global_err/bad_settings0.in = primer3-2.3.6/test/primer_global_err/mispr_obj_fn.in0000664000077300007730000000040712231156245021523 0ustar ioanaioanaSEQUENCE_ID=mispr_lib_not_defined PRIMER_WT_LIBRARY_MISPRIMING=1 SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT = primer3-2.3.6/test/primer_global_err/no_terminal_equal.out0000664000077300007730000000064212231156245022754 0ustar ioanaioanaSEQUENCE_ID=example SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 PRIMER_PRODUCT_SIZE_RANGE=75-100 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_ERROR=Final record not terminated by '=' = primer3-2.3.6/test/primer_global_err/must_match_length_10.in0000664000077300007730000000043512231156245023062 0ustar ioanaioanaSEQUENCE_ID=must_match_length_10 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_THREE_PRIME=NQNNN = primer3-2.3.6/test/primer_global_err/pr_gc_content_obj_fn.out0000664000077300007730000000160012231156245023412 0ustar ioanaioanaSEQUENCE_ID=primer_gc_content PRIMER_WT_SEQ_QUAL=0 PRIMER_INTERNAL_WT_SEQ_QUAL=0 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 PRIMER_MIN_GC=40 PRIMER_MAX_GC=60 PRIMER_WT_GC_PERCENT_LT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PAIR_WT_PR_PENALTY=1 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG PRIMER_ERROR=Primer GC content is part of objective function while optimum gc_content is not defined = primer3-2.3.6/test/primer_global_err/repeat_lib_err2.in0000664000077300007730000000034012231156245022110 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib2 = primer3-2.3.6/test/primer_global_err/incorrect_task.out0000664000077300007730000000046712231156245022275 0ustar ioanaioanaSEQUENCE_ID=incorrect_task PRIMER_TASK=pick_pcr_primers_and_internal_oligo SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT PRIMER_ERROR=Unrecognized PRIMER_TASK = primer3-2.3.6/test/primer_global_err/bad_first_base_index.out20000664000077300007730000000010412231156245023447 0ustar ioanaioana../src/primer3_core: Value too small at tag PRIMER_FIRST_BASE_INDEX primer3-2.3.6/test/primer_global_err/pair_mispr.out20000664000077300007730000000015312231156245021502 0ustar ioanaioana../src/primer3_core: Mispriming score is part of objective function, but mispriming library is not defined primer3-2.3.6/test/primer_global_err/must_match_length_4.in0000664000077300007730000000044412231156245023005 0ustar ioanaioanaSEQUENCE_ID=must_match_length_4 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=NNNN = primer3-2.3.6/test/primer_global_err/bad_product_sizes_2.out20000664000077300007730000000010112231156245023252 0ustar ioanaioana../src/primer3_core: Illegal PRIMER_PRODUCT_SIZE_RANGE value: 23 primer3-2.3.6/test/primer_global_err/too_many_product_sizes.out20000664000077300007730000000010312231156245024132 0ustar ioanaioana../src/primer3_core: Too many values for PRIMER_PRODUCT_SIZE_RANGE primer3-2.3.6/test/primer_global_err/bad_product_sizes_1.out0000664000077300007730000000052112231156245023175 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12 PRIMER_PRODUCT_SIZE_RANGE=75-100-23 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_ERROR=Illegal PRIMER_PRODUCT_SIZE_RANGE value: -23 = primer3-2.3.6/test/primer_global_err/bad_float_4.out20000664000077300007730000000010312231156245021466 0ustar ioanaioana../src/primer3_core: Illegal PRIMER_PICK_INTERNAL_OLIGO value: 1.0 primer3-2.3.6/test/primer_global_err/bad_gc_1.out0000664000077300007730000000047512231156245020701 0ustar ioanaioanaSEQUENCE_ID=bad_gc_1 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_GC=-2 PRIMER_ERROR=Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC = primer3-2.3.6/test/primer_global_err/must_match_length_8.in0000664000077300007730000000044612231156245023013 0ustar ioanaioanaSEQUENCE_ID=must_match_length_8 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=NNNNNN = primer3-2.3.6/test/primer_global_err/repeat_lib_err6.out0000664000077300007730000000043612231156245022323 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib6 PRIMER_ERROR=Illegal weight in mispriming library repeat_lib6 = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err.out20000664000077300007730000000011312231156245023161 0ustar ioanaioana../src/primer3_core: Cannot open internal oligo mishyb library repeat_file primer3-2.3.6/test/primer_global_err/pr_gc_content_obj_fn.in0000664000077300007730000000143312231156245023215 0ustar ioanaioanaSEQUENCE_ID=primer_gc_content PRIMER_WT_SEQ_QUAL=0 PRIMER_INTERNAL_WT_SEQ_QUAL=0 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 PRIMER_MIN_GC=40 PRIMER_MAX_GC=60 PRIMER_WT_GC_PERCENT_LT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PAIR_WT_PR_PENALTY=1 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG = primer3-2.3.6/test/primer_global_err/opt_max_size_int.out20000664000077300007730000000010312231156245022703 0ustar ioanaioana../src/primer3_core: PRIMER_INTERNAL_{OPT,DEFAULT}_SIZE > MAX_SIZE primer3-2.3.6/test/primer_global_err/max_size_limit_int.out20000664000077300007730000000010712231156245023223 0ustar ioanaioana../src/primer3_core: PRIMER_INTERNAL_MAX_SIZE exceeds built-in maximum primer3-2.3.6/test/primer_global_err/io_obj_fn.out0000664000077300007730000000055412231156245021204 0ustar ioanaioanaSEQUENCE_ID=io_not_required PRIMER_PAIR_WT_IO_PENALTY=1 SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT PRIMER_ERROR=Internal oligo quality is part of objective function while internal oligo choice is not required = primer3-2.3.6/test/primer_global_err/bad_gc_int_3.in0000664000077300007730000000044512231156245021351 0ustar ioanaioanaSEQUENCE_ID=bad_gc_int_3 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_GC=60 PRIMER_INTERNAL_MIN_GC=70 = primer3-2.3.6/test/primer_global_err/bad_min_three_prime_distance.out20000664000077300007730000000016512231156245025166 0ustar ioanaioana../src/primer3_core: Both PRIMER_MIN_THREE_PRIME_DISTANCE and PRIMER_{LEFT/RIGHT}_MIN_THREE_PRIME_DISTANCE specified primer3-2.3.6/test/primer_global_err/pair_mispr.out0000664000077300007730000000054712231156245021427 0ustar ioanaioanaSEQUENCE_ID=mispr_library PRIMER_PAIR_WT_LIBRARY_MISPRIMING=1 SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT PRIMER_ERROR=Mispriming score is part of objective function, but mispriming library is not defined = primer3-2.3.6/test/primer_global_err/bad_product_sizes_3.out0000664000077300007730000000052112231156245023177 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12 PRIMER_PRODUCT_SIZE_RANGE=75-100,23 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_ERROR=Illegal PRIMER_PRODUCT_SIZE_RANGE value: ,23 = primer3-2.3.6/test/primer_global_err/must_match_length_5.in0000664000077300007730000000043412231156245023005 0ustar ioanaioanaSEQUENCE_ID=must_match_length_5 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_FIVE_PRIME=NNNNNN = primer3-2.3.6/test/primer_global_err/bad_float_3.in0000664000077300007730000000042212231156245021206 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END= PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 = primer3-2.3.6/test/primer_global_err/max_opt_tm_int.out20000664000077300007730000000013112231156245022352 0ustar ioanaioana../src/primer3_core: Optimum internal oligo Tm lower than minimum or higher than maximum primer3-2.3.6/test/primer_global_err/bad_product_sizes_4.out0000664000077300007730000000052112231156245023200 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12 PRIMER_PRODUCT_SIZE_RANGE=75,100 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_ERROR=Illegal PRIMER_PRODUCT_SIZE_RANGE value: 75,100 = primer3-2.3.6/test/primer_global_err/max_size_limit_int.out0000664000077300007730000000052212231156245023142 0ustar ioanaioanaSEQUENCE_ID=max_size_limit_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_SIZE=45 PRIMER_ERROR=PRIMER_INTERNAL_MAX_SIZE exceeds built-in maximum = primer3-2.3.6/test/primer_global_err/must_match_length_6.in0000664000077300007730000000043512231156245023007 0ustar ioanaioanaSEQUENCE_ID=must_match_length_6 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_THREE_PRIME=NNNNNN = primer3-2.3.6/test/primer_global_err/bad_gc_1.out20000664000077300007730000000010712231156245020753 0ustar ioanaioana../src/primer3_core: Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC primer3-2.3.6/test/primer_global_err/must_match_length_0.in0000664000077300007730000000042612231156245023001 0ustar ioanaioanaSEQUENCE_ID=must_match_length_0 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_FIVE_PRIME= = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err.out0000664000077300007730000000050512231156245023104 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_file PRIMER_ERROR=Cannot open internal oligo mishyb library repeat_file = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err2.in0000664000077300007730000000040212231156245022761 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib2 PRIMER_PICK_INTERNAL_OLIGO=1 = primer3-2.3.6/test/primer_global_err/max_min_tm.out20000664000077300007730000000012112231156245021460 0ustar ioanaioana../src/primer3_core: Optimum primer Tm lower than minimum or higher than maximum primer3-2.3.6/test/primer_global_err/io_gc_content_obj_fn.out20000664000077300007730000000016012231156245023462 0ustar ioanaioana../src/primer3_core: Hyb probe GC content is part of objective function while optimum gc_content is not defined primer3-2.3.6/test/primer_global_err/bad_self_end.in0000664000077300007730000000041012231156245021433 0ustar ioanaioanaSEQUENCE_ID=bad_self_end SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_SELF_END=-1 = primer3-2.3.6/test/primer_global_err/bad_settings1.in0000664000077300007730000000003512231156245021600 0ustar ioanaioanaThis is a bad settings file. primer3-2.3.6/test/primer_global_err/bad_settings0.out20000664000077300007730000000012212231156245022057 0ustar ioanaioana../src/primer3_core: Settings file is empty: ./primer_global_err/bad_settings0.in primer3-2.3.6/test/primer_global_err/repeat_lib_err1.in0000664000077300007730000000034012231156245022107 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib1 = primer3-2.3.6/test/primer_global_err/max_opt_tm.out20000664000077300007730000000012112231156245021477 0ustar ioanaioana../src/primer3_core: Optimum primer Tm lower than minimum or higher than maximum primer3-2.3.6/test/primer_global_err/bad_gc_int_2.out0000664000077300007730000000050412231156245021545 0ustar ioanaioanaSEQUENCE_ID=bad_gc_int_2 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MIN_GC=101 PRIMER_ERROR=Illegal value for PRIMER_INTERNAL_OLIGO_GC = primer3-2.3.6/test/primer_global_err/bad_gc_3.out20000664000077300007730000000010712231156245020755 0ustar ioanaioana../src/primer3_core: Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC primer3-2.3.6/test/primer_global_err/product_size_obj_fn.out0000664000077300007730000000054612231156245023310 0ustar ioanaioanaSEQUENCE_ID=product_size_opt_not_def PRIMER_PAIR_WT_PRODUCT_SIZE_LT=1 SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT PRIMER_ERROR=Product size is part of objective function while optimum size is not defined = primer3-2.3.6/test/primer_global_err/gc_clamp_min_size.out20000664000077300007730000000006712231156245023003 0ustar ioanaioana../src/primer3_core: PRIMER_GC_CLAMP > PRIMER_MIN_SIZE primer3-2.3.6/test/primer_global_err/bad_dna_conc.out0000664000077300007730000000050412231156245021625 0ustar ioanaioanaSEQUENCE_ID=bad_dna_conc SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_DNA_CONC=-1 PRIMER_ERROR=Illegal value for primer salt or dna concentration = primer3-2.3.6/test/primer_global_err/no_terminal_equal.in0000664000077300007730000000056012231156245022552 0ustar ioanaioanaSEQUENCE_ID=example SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 PRIMER_PRODUCT_SIZE_RANGE=75-100 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 primer3-2.3.6/test/primer_global_err/bad_float_1.in0000664000077300007730000000042712231156245021211 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=-12.0 PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 = primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_4.in0000664000077300007730000000043612231156245023744 0ustar ioanaioanaPRIMER_MAX_TM=100 PRIMER_MIN_TM=0 SEQUENCE_ID=oligotm_2 PRIMER_SALT_DIVALENT=1 PRIMER_DNTP_CONC=-1 P3_COMMENT=Should succeed SEQUENCE_TEMPLATE=ACAAGATAATGTTTTGAACATTGTCCTGTTGGTTTCTTTGAAGCAGACCAGAAGTTAAAAAGTTAGAGATCCATAGTGGGTGACAGTCCCGTATTCGAAATCTCAACAGCGGTACTAAGCGTACGAAAAGTAGGGCGGGG = primer3-2.3.6/test/primer_global_err/gc_clamp_min_size.out0000664000077300007730000000051312231156245022715 0ustar ioanaioanaSEQUENCE_ID=gc_clamp_min_size SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_GC_CLAMP=19 PRIMER_MIN_SIZE=18 PRIMER_ERROR=PRIMER_GC_CLAMP > PRIMER_MIN_SIZE = primer3-2.3.6/test/primer_global_err/max_min_tm.out0000664000077300007730000000055312231156245021407 0ustar ioanaioanaSEQUENCE_ID=max_min_tm SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MIN_TM=65 PRIMER_OPT_TM=60 PRIMER_MAX_TM=62 PRIMER_ERROR=Optimum primer Tm lower than minimum or higher than maximum = primer3-2.3.6/test/primer_global_err/opt_min_size.in0000664000077300007730000000045212231156245021553 0ustar ioanaioanaSEQUENCE_ID=opt_min_size SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_OPT_SIZE=15 PRIMER_MAX_SIZE=20 PRIMER_MIN_SIZE=18 = primer3-2.3.6/test/primer_global_err/bad_self_any.in0000664000077300007730000000041012231156245021454 0ustar ioanaioanaSEQUENCE_ID=bad_self_any SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_SELF_ANY=-1 = primer3-2.3.6/test/primer_global_err/bad_settings6.out0000664000077300007730000000016512231156245022012 0ustar ioanaioanaPRIMER_ERROR=Unknown file type in at line 2 (line2='P3_FILE_TYPE=illegal') in ./primer_global_err/bad_settings6.in = primer3-2.3.6/test/primer_global_err/bad_salt_conc.out20000664000077300007730000000011012231156245022101 0ustar ioanaioana../src/primer3_core: Illegal value for primer salt or dna concentration primer3-2.3.6/test/primer_global_err/bad_float_3.out0000664000077300007730000000050312231156245021407 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END= PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_ERROR=Illegal PRIMER_MAX_SELF_END value: = primer3-2.3.6/test/primer_global_err/must_match_length_3.in0000664000077300007730000000044312231156245023003 0ustar ioanaioanaSEQUENCE_ID=must_match_length_3 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=NNNN = primer3-2.3.6/test/primer_global_err/bad_settings4.in0000664000077300007730000000072512231156245021611 0ustar ioanaioanaPrimer3 File - http://primer3.sourceforge.net PRIMER_FIRST_BASE_INDEX=1 PRIMER_TASK=pick_pcr_primers P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers PRIMER3_MIN_THREE_PRIME_DISTANCE=3 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=300-400 401-500 501-600 601-700 701-850 851-1001 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00 = primer3-2.3.6/test/primer_global_err/io_obj_fn.out20000664000077300007730000000016612231156245021265 0ustar ioanaioana../src/primer3_core: Internal oligo quality is part of objective function while internal oligo choice is not required primer3-2.3.6/test/primer_global_err/seq_quality1.in0000664000077300007730000000112312231156245021471 0ustar ioanaioanaSEQUENCE_ID=quality_1 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_MIN_QUALITY=20 PRIMER_QUALITY_RANGE_MIN=30 = primer3-2.3.6/test/primer_global_err/bad_self_end.out0000664000077300007730000000051312231156245021640 0ustar ioanaioanaSEQUENCE_ID=bad_self_end SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_SELF_END=-1 PRIMER_ERROR=Illegal value for primer complementarity restrictions = primer3-2.3.6/test/primer_global_err/opt_min_size.out0000664000077300007730000000054312231156245021755 0ustar ioanaioanaSEQUENCE_ID=opt_min_size SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_OPT_SIZE=15 PRIMER_MAX_SIZE=20 PRIMER_MIN_SIZE=18 PRIMER_ERROR=PRIMER_{OPT,DEFAULT}_SIZE < PRIMER_MIN_SIZE = primer3-2.3.6/test/primer_global_err/must_match_length_3.out0000664000077300007730000000055512231156245023210 0ustar ioanaioanaSEQUENCE_ID=must_match_length_3 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=NNNN PRIMER_ERROR=PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME must have 5 characters = primer3-2.3.6/test/primer_global_err/opt_min_size_int.out0000664000077300007730000000060412231156245022625 0ustar ioanaioanaSEQUENCE_ID=opt_min_size_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_OPT_SIZE=15 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_ERROR=PRIMER_INTERNAL_{OPT,DEFAULT}_SIZE < MIN_SIZE = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err4.out20000664000077300007730000000012112231156245023244 0ustar ioanaioana../src/primer3_core: Empty sequence in internal oligo mishyb library repeat_lib4 primer3-2.3.6/test/primer_global_err/must_match_length_9.in0000664000077300007730000000043412231156245023011 0ustar ioanaioanaSEQUENCE_ID=must_match_length_9 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_THREE_PRIME=NNZNN = primer3-2.3.6/test/primer_global_err/must_match_length_3.out20000664000077300007730000000012212231156245023260 0ustar ioanaioana../src/primer3_core: PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME must have 5 characters primer3-2.3.6/test/primer_global_err/product_size_obj_fn.in0000664000077300007730000000041412231156245023101 0ustar ioanaioanaSEQUENCE_ID=product_size_opt_not_def PRIMER_PAIR_WT_PRODUCT_SIZE_LT=1 SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT = primer3-2.3.6/test/primer_global_err/input_for_settings_tests.txt0000664000077300007730000000205712231156245024437 0ustar ioanaioanaSEQUENCE_ID=chr16-730958 SEQUENCE_TEMPLATE=TGCAAAGGCTGTGGCTTGGGGGTCCTGAGGCCTGACAAGGCGTCCTGCGCAGGGGAAGGGGAAGAGGCCCCGGCCCGGGCCCCGCCGCCCTCCAGCTGCGACCCAGGGGTCGGGTTAGGGTTAGGGATGTGTGGGAGCCGCCTCTGCTGCCTCCTCTGGGTTGGGCAGGCCGGCGCGAGCCCGCGACCACGGGACCCTCCCACGCAGCCCGCCCGGACACCATGGGGCACAGGAGCGGGTTGGGGGGCGCCGCCCTCCCTGGCCTCAGTTTCCTTGTTGGTAAGAAGCGGGCCTCAGTGTCTGAGGCCCCGCGCCCGGGTCGATAGAGTCCCTGACGGCCCAGACCGGGCTCCCGGGATTCGGATCTCATTCCTCAGGGGAGGAAGTGGGGCGCAGAGCAATTTCCCTCCGCGCGACAGGGCACCGAGCGCAGCCTCGACCCCGCCCGCCCAGGCCGGCCCACCTGAGACGGCCCGATGAAGTCATCCAGGTCCGTCAGCTGCAGCGCCCCGCTGAAGGGCGACGCCATGACGGCCGCACTGCCGCCGCGCGCAGGTGTCGCTAAACGGCGCGAAACGAAGTGGCTGGTTCCGCCACGCTGCCGGAAAGCGCCCCCAGGACGCATGCGCACCTCGTCCCGGGGCCGGCGCCCTCTGCTGACGAGCCCCGGCGCTGCGGCTCCCCGCCCACCTCGCGGGAACCCGGGACCTACCGCTTCGTCCACCTCCCGCGCGCCCCAGGCCCCGGGTTGGGGTGCAGGGGGCGCAGGGGTTGGTCGGGCCAGCGCCGCAGCTCCGGCCCCGGAGTCTCTCCGCCTTCACAAGGAAGCGGAGGGGATCTGGCTCCGTCCATTTCCTCATCCTCTGCGTACCTGCGGACCAGGTGCTCCCGGATGCAACCCAAGAACCCGGAACGCGCCTGGCACACAGGAGGCGCTCAGCTCGTGTTGGGGAATTCAGGCAGGGAAGAAAGCATGGATAGGCACGAGGTCTCCCCTTCCT SEQUENCE_TARGET=350,301 = primer3-2.3.6/test/primer_global_err/bad_self_end_int.out20000664000077300007730000000012312231156245022571 0ustar ioanaioana../src/primer3_core: Illegal value for internal oligo complementarity restrictions primer3-2.3.6/test/primer_global_err/bad_settings6.in0000664000077300007730000000075112231156245021612 0ustar ioanaioanaPrimer3 File - http://primer3.sourceforge.net P3_FILE_TYPE=illegal PRIMER_FIRST_BASE_INDEX=1 PRIMER_TASK=pick_pcr_primers P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers PRIMER3_MIN_THREE_PRIME_DISTANCE=3 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=300-400 401-500 501-600 601-700 701-850 851-1001 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00 = primer3-2.3.6/test/primer_global_err/bad_self_end_int.in0000664000077300007730000000042512231156245022313 0ustar ioanaioanaSEQUENCE_ID=bad_self_end_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_SELF_END=-1 = primer3-2.3.6/test/primer_global_err/bad_product_sizes_4.in0000664000077300007730000000042412231156245023001 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12 PRIMER_PRODUCT_SIZE_RANGE=75,100 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 = primer3-2.3.6/test/primer_global_err/max_min_tm_int.in0000664000077300007730000000050112231156245022051 0ustar ioanaioanaSEQUENCE_ID=max_min_tm_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MIN_TM=65 PRIMER_INTERNAL_OPT_TM=60 PRIMER_INTERNAL_MAX_TM=62 = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err.in0000664000077300007730000000040212231156245022677 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_file = primer3-2.3.6/test/primer_global_err/product_tm_obj_fn.out0000664000077300007730000000056012231156245022752 0ustar ioanaioanaSEQUENCE_ID=product_tm_opt_not_def PRIMER_PAIR_WT_PRODUCT_TM_LT=1 SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT PRIMER_ERROR=Product temperature is part of objective function while optimum temperature is not defined = primer3-2.3.6/test/primer_global_err/bad_settings3.in0000664000077300007730000000067512231156245021614 0ustar ioanaioanaP3_FILE_TYPE=settings PRIMER_FIRST_BASE_INDEX=1 PRIMER_TASK=pick_pcr_primers P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers PRIMER3_MIN_THREE_PRIME_DISTANCE=3 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=300-400 401-500 501-600 601-700 701-850 851-1001 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00 = primer3-2.3.6/test/primer_global_err/min_opt_tm.in0000664000077300007730000000044212231156245021220 0ustar ioanaioanaSEQUENCE_ID=min_opt_tm SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_TM=65 PRIMER_OPT_TM=60 PRIMER_MIN_TM=62 = primer3-2.3.6/test/primer_global_err/max_min_tm_int.out0000664000077300007730000000062212231156245022256 0ustar ioanaioanaSEQUENCE_ID=max_min_tm_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MIN_TM=65 PRIMER_INTERNAL_OPT_TM=60 PRIMER_INTERNAL_MAX_TM=62 PRIMER_ERROR=Optimum internal oligo Tm lower than minimum or higher than maximum = primer3-2.3.6/test/primer_global_err/max_size_min_product.out20000664000077300007730000000010512231156245023554 0ustar ioanaioana../src/primer3_core: PRIMER_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE primer3-2.3.6/test/primer_global_err/bad_gc_2.out20000664000077300007730000000010712231156245020754 0ustar ioanaioana../src/primer3_core: Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC primer3-2.3.6/test/primer_global_err/bad_num_ns_int.in0000664000077300007730000000042612231156245022034 0ustar ioanaioanaSEQUENCE_ID=bad_num_ns_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_NS_ACCEPTED=-1 = primer3-2.3.6/test/primer_global_err/opt_max_size.out20000664000077300007730000000010112231156245022027 0ustar ioanaioana../src/primer3_core: PRIMER_{OPT,DEFAULT}_SIZE > PRIMER_MAX_SIZE primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_2.in0000664000077300007730000000061212231156245023736 0ustar ioanaioanaPRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_ID=oligotm_io_2 SEQUENCE_TEMPLATE=CGTCGGTGCGGAAACTTATCGGGTAAAAAGGTTTCTTTAGTTTGGTGTCGATTTTGTCGGTGCCGGTGGCGCAGAACCATTTTACCGAACCGTTTTGACGCTTGGCCCACAGGGAGAGTTTTTTGCCTTGGATTTCTTTG PRIMER_INTERNAL_SALT_DIVALENT=1 PRIMER_INTERNAL_DNTP_CONC=-1 P3_COMMENT=Should fail = primer3-2.3.6/test/primer_global_err/repeat_lib_err5.out0000664000077300007730000000043612231156245022322 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib5 PRIMER_ERROR=Illegal weight in mispriming library repeat_lib5 = primer3-2.3.6/test/primer_global_err/max_opt_tm.out0000664000077300007730000000055312231156245021426 0ustar ioanaioanaSEQUENCE_ID=max_opt_tm SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_TM=65 PRIMER_OPT_TM=66 PRIMER_MIN_TM=62 PRIMER_ERROR=Optimum primer Tm lower than minimum or higher than maximum = primer3-2.3.6/test/primer_global_err/min_opt_tm_int.out20000664000077300007730000000013112231156245022350 0ustar ioanaioana../src/primer3_core: Optimum internal oligo Tm lower than minimum or higher than maximum primer3-2.3.6/test/primer_global_err/mispr_obj_fn.out20000664000077300007730000000015312231156245022004 0ustar ioanaioana../src/primer3_core: Mispriming score is part of objective function, but mispriming library is not defined primer3-2.3.6/test/primer_global_err/repeat_lib_err.out0000664000077300007730000000043012231156245022227 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_file PRIMER_ERROR=Cannot open mispriming library repeat_file = primer3-2.3.6/test/primer_global_err/bad_first_base_index.out0000664000077300007730000000052612231156245023375 0ustar ioanaioanaSEQUENCE_ID=bad_first_base_index SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_FIRST_BASE_INDEX=-1000005 PRIMER_ERROR=Value too small at tag PRIMER_FIRST_BASE_INDEX = primer3-2.3.6/test/primer_global_err/max_min_tm_int.out20000664000077300007730000000013112231156245022333 0ustar ioanaioana../src/primer3_core: Optimum internal oligo Tm lower than minimum or higher than maximum primer3-2.3.6/test/primer_global_err/repeat_lib_err5.out20000664000077300007730000000010612231156245022376 0ustar ioanaioana../src/primer3_core: Illegal weight in mispriming library repeat_lib5 primer3-2.3.6/test/primer_global_err/seq_quality3.out20000664000077300007730000000011412231156245021755 0ustar ioanaioana../src/primer3_core: PRIMER_INTERNAL_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN primer3-2.3.6/test/primer_global_err/mishyb_obj_fn.in0000664000077300007730000000047012231156245021664 0ustar ioanaioanaSEQUENCE_ID=mishyb_lib_not_defined PRIMER_INTERNAL_WT_LIBRARY_MISHYB=1 PRIMER_TASK=pick_pcr_primers_and_hyb_probe SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT = primer3-2.3.6/test/primer_global_err/repeat_lib_err5.in0000664000077300007730000000034012231156245022113 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib5 = primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_1.in0000664000077300007730000000061212231156245023735 0ustar ioanaioanaSEQUENCE_ID=oligotm_io_1 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=GTCGCCCGGCTGCGCGCCTGTATCCACATCCAAGAGCTTCAGTTTCCCGTCTGCCGTGGCGGCACTCAAATTTTCGTCCACCAGGGCAAGCGGCCCGTGCTTCAGTTCGCCGGCAGGATAGGCTTCGGCGTGGATGTAGG PRIMER_INTERNAL_SALT_DIVALENT=-1 PRIMER_INTERNAL_DNTP_CONC=1 P3_COMMENT=Should fail PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_PICK_INTERNAL_OLIGO=1 = primer3-2.3.6/test/primer_global_err/must_match_length_5.out20000664000077300007730000000011112231156245023260 0ustar ioanaioana../src/primer3_core: PRIMER_MUST_MATCH_FIVE_PRIME must have 5 characters primer3-2.3.6/test/primer_global_err/repeat_lib_err6.in0000664000077300007730000000034012231156245022114 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib6 = primer3-2.3.6/test/primer_global_err/bad_settings0.in0000664000077300007730000000000012231156245021567 0ustar ioanaioanaprimer3-2.3.6/test/primer_global_err/repeat_lib_err.in0000664000077300007730000000034012231156245022026 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_file = primer3-2.3.6/test/primer_global_err/max_min_tm.in0000664000077300007730000000044212231156245021203 0ustar ioanaioanaSEQUENCE_ID=max_min_tm SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MIN_TM=65 PRIMER_OPT_TM=60 PRIMER_MAX_TM=62 = primer3-2.3.6/test/primer_global_err/bad_settings5.out0000664000077300007730000000006612231156245022011 0ustar ioanaioanaPRIMER_ERROR=Unrecognized tag: PRIMER_NO_SUCH_TAG=3 = primer3-2.3.6/test/primer_global_err/empty_product_size.in0000664000077300007730000000042212231156245023001 0ustar ioanaioanaSEQUENCE_ID=empty_product_size SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_PRODUCT_SIZE_RANGE= = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err5.in0000664000077300007730000000040212231156245022764 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib5 PRIMER_PICK_INTERNAL_OLIGO=1 = primer3-2.3.6/test/primer_global_err/min_opt_tm.out20000664000077300007730000000012112231156245021475 0ustar ioanaioana../src/primer3_core: Optimum primer Tm lower than minimum or higher than maximum primer3-2.3.6/test/primer_global_err/no_equal.in0000664000077300007730000000026512231156245020661 0ustar ioanaioanaSEQUENCE_ID=without_eq AGTCGATCGTAGCTAGCTAGCTAGCTGACTGATGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTGATCGTAGCTAGCTAGCTGACTGATCATCTAGCTAGCTGATCATCGTAGCTAGCTCTATTTTATCGTAGCTAGCTATCATCTAGCTAGC = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err4.out0000664000077300007730000000051312231156245023167 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib4 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_ERROR=Empty sequence in internal oligo mishyb library repeat_lib4 = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err5.out0000664000077300007730000000051312231156245023170 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib5 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_ERROR=Illegal weight in internal oligo mishyb library repeat_lib5 = primer3-2.3.6/test/primer_global_err/bad_gc_2.out0000664000077300007730000000047612231156245020703 0ustar ioanaioanaSEQUENCE_ID=bad_gc_2 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MIN_GC=101 PRIMER_ERROR=Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err3.out0000664000077300007730000000051312231156245023166 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib3 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_ERROR=Empty sequence in internal oligo mishyb library repeat_lib3 = primer3-2.3.6/test/primer_global_err/seq_quality4.out0000664000077300007730000000127512231156245021705 0ustar ioanaioanaSEQUENCE_ID=quality_1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=20 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_QUALITY_RANGE_MAX=10 PRIMER_ERROR=PRIMER_INTERNAL_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX = primer3-2.3.6/test/primer_global_err/incorrect_task.out20000664000077300007730000000005612231156245022351 0ustar ioanaioana../src/primer3_core: Unrecognized PRIMER_TASK primer3-2.3.6/test/primer_global_err/max_size_min_product_int.out0000664000077300007730000000100212231156245024341 0ustar ioanaioanaSEQUENCE_ID=max_size_min_product_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_SIZE=24 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_PRODUCT_SIZE_RANGE=24-50 50-75 75-100 PRIMER_ERROR=PRIMER_INTERNAL_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE = primer3-2.3.6/test/primer_global_err/bad_self_any_int.out0000664000077300007730000000054012231156245022533 0ustar ioanaioanaSEQUENCE_ID=bad_self_any_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_SELF_ANY=-1 PRIMER_ERROR=Illegal value for internal oligo complementarity restrictions = primer3-2.3.6/test/primer_global_err/opt_min_size_int.out20000664000077300007730000000010312231156245022701 0ustar ioanaioana../src/primer3_core: PRIMER_INTERNAL_{OPT,DEFAULT}_SIZE < MIN_SIZE primer3-2.3.6/test/primer_global_err/must_match_length_7.out20000664000077300007730000000012212231156245023264 0ustar ioanaioana../src/primer3_core: PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME must have 5 characters primer3-2.3.6/test/primer_global_err/zero_min_size.out20000664000077300007730000000006212231156245022210 0ustar ioanaioana../src/primer3_core: PRIMER_MIN_SIZE must be >= 1 primer3-2.3.6/test/primer_global_err/seq_quality2.out0000664000077300007730000000121612231156245021676 0ustar ioanaioanaSEQUENCE_ID=quality_1 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_MIN_QUALITY=20 PRIMER_QUALITY_RANGE_MAX=10 PRIMER_ERROR=PRIMER_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX = primer3-2.3.6/test/primer_global_err/bad_gc_2.in0000664000077300007730000000037712231156245020502 0ustar ioanaioanaSEQUENCE_ID=bad_gc_2 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MIN_GC=101 = primer3-2.3.6/test/primer_global_err/max_opt_tm.in0000664000077300007730000000044212231156245021222 0ustar ioanaioanaSEQUENCE_ID=max_opt_tm SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_TM=65 PRIMER_OPT_TM=66 PRIMER_MIN_TM=62 = primer3-2.3.6/test/primer_global_err/max_size_limit_int.in0000664000077300007730000000042312231156245022741 0ustar ioanaioanaSEQUENCE_ID=max_size_limit_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_SIZE=45 = primer3-2.3.6/test/primer_global_err/must_match_length_0.out20000664000077300007730000000011112231156245023253 0ustar ioanaioana../src/primer3_core: PRIMER_MUST_MATCH_FIVE_PRIME must have 5 characters primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_1.out20000664000077300007730000000013212231156245024215 0ustar ioanaioana../src/primer3_core: Illegal value for internal oligo divalent salt or dNTP concentration primer3-2.3.6/test/primer_global_err/min_opt_tm_int.out0000664000077300007730000000062212231156245022273 0ustar ioanaioanaSEQUENCE_ID=min_opt_tm_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_TM=65 PRIMER_INTERNAL_OPT_TM=60 PRIMER_INTERNAL_MIN_TM=62 PRIMER_ERROR=Optimum internal oligo Tm lower than minimum or higher than maximum = primer3-2.3.6/test/primer_global_err/opt_max_size_int.out0000664000077300007730000000060412231156245022627 0ustar ioanaioanaSEQUENCE_ID=opt_max_size_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_ERROR=PRIMER_INTERNAL_{OPT,DEFAULT}_SIZE > MAX_SIZE = primer3-2.3.6/test/primer_global_err/bad_min_three_prime_distance.in0000664000077300007730000000110412231156245024675 0ustar ioanaioanaSEQUENCE_ID=error, both general and specific tag in same record PRIMER_PRODUCT_SIZE_RANGE=100-500 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_NUM_RETURN=40 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA =primer3-2.3.6/test/primer_global_err/repeat_lib_int_err5.out20000664000077300007730000000012112231156245023245 0ustar ioanaioana../src/primer3_core: Illegal weight in internal oligo mishyb library repeat_lib5 primer3-2.3.6/test/primer_global_err/too_many_product_sizes.in0000664000077300007730000000314212231156245023655 0ustar ioanaioanaSEQUENCE_ID=global_parameter_error_00 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (testing number of product size ranges) = primer3-2.3.6/test/primer_global_err/MAX_PRIMER_LENGTH.out0000664000077300007730000000065612231156245022027 0ustar ioanaioanaSEQUENCE_ID=boundary_test_87 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=37 PRIMER_MAX_SIZE=37 PRIMER_OPT_SIZE=37 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=77-77 SEQUENCE_TEMPLATE=NNCACGTCATCATATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATATCTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (testing hard limit MAX_PRIMER_LENGTH) PRIMER_ERROR=PRIMER_MAX_SIZE exceeds built-in maximum of 36 = primer3-2.3.6/test/primer_global_err/must_match_length_8.out0000664000077300007730000000056112231156245023212 0ustar ioanaioanaSEQUENCE_ID=must_match_length_8 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=NNNNNN PRIMER_ERROR=PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME must have 5 characters = primer3-2.3.6/test/primer_global_err/junction_overlap_min_size.out0000664000077300007730000000463112231156245024536 0ustar ioanaioanaSEQUENCE_ID=Medicago Lyk3 mRNA INTRON_OVERLAP error small SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 SEQUENCE_OVERLAP_JUNCTION_LIST=10 PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION=0 PRIMER_ERROR=Illegal value for PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION = primer3-2.3.6/test/primer_global_err/must_match_length_8.out20000664000077300007730000000012312231156245023266 0ustar ioanaioana../src/primer3_core: PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME must have 5 characters primer3-2.3.6/test/primer_global_err/opt_max_size.out0000664000077300007730000000054312231156245021757 0ustar ioanaioanaSEQUENCE_ID=opt_max_size SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_OPT_SIZE=25 PRIMER_MAX_SIZE=20 PRIMER_MIN_SIZE=18 PRIMER_ERROR=PRIMER_{OPT,DEFAULT}_SIZE > PRIMER_MAX_SIZE = primer3-2.3.6/test/primer_global_err/bad_float_2.out0000664000077300007730000000051712231156245021413 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12.0.4 PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_ERROR=Illegal PRIMER_MAX_SELF_END value: 12.0.4 = primer3-2.3.6/test/primer_global_err/min_opt_tm.out0000664000077300007730000000055312231156245021424 0ustar ioanaioanaSEQUENCE_ID=min_opt_tm SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_TM=65 PRIMER_OPT_TM=60 PRIMER_MIN_TM=62 PRIMER_ERROR=Optimum primer Tm lower than minimum or higher than maximum = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err1.out20000664000077300007730000000014112231156245023243 0ustar ioanaioana../src/primer3_core: Missing id line (expected '>') in internal oligo mishyb library repeat_lib1 primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_3.out20000664000077300007730000000012212231156245024216 0ustar ioanaioana../src/primer3_core: Illegal value for primer divalent salt or dNTP concentration primer3-2.3.6/test/primer_global_err/bad_num_ns_int.out20000664000077300007730000000010712231156245022313 0ustar ioanaioana../src/primer3_core: Illegal value for PRIMER_INTERNAL_MAX_NS_ACCEPTED primer3-2.3.6/test/primer_global_err/product_size_obj_fn.out20000664000077300007730000000014212231156245023362 0ustar ioanaioana../src/primer3_core: Product size is part of objective function while optimum size is not defined primer3-2.3.6/test/primer_global_err/must_match_length_2.out0000664000077300007730000000053512231156245023205 0ustar ioanaioanaSEQUENCE_ID=must_match_length_2 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_THREE_PRIME=NNNN PRIMER_ERROR=PRIMER_MUST_MATCH_THREE_PRIME must have 5 characters = primer3-2.3.6/test/primer_global_err/opt_min_size.out20000664000077300007730000000010112231156245022025 0ustar ioanaioana../src/primer3_core: PRIMER_{OPT,DEFAULT}_SIZE < PRIMER_MIN_SIZE primer3-2.3.6/test/primer_global_err/must_match_length_9.out20000664000077300007730000000010612231156245023270 0ustar ioanaioana../src/primer3_core: Illegal values for PRIMER_MUST_MATCH_THREE_PRIME primer3-2.3.6/test/primer_global_err/seq_quality1.out0000664000077300007730000000121612231156245021675 0ustar ioanaioanaSEQUENCE_ID=quality_1 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_MIN_QUALITY=20 PRIMER_QUALITY_RANGE_MIN=30 PRIMER_ERROR=PRIMER_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err3.in0000664000077300007730000000040212231156245022762 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib3 PRIMER_PICK_INTERNAL_OLIGO=1 = primer3-2.3.6/test/primer_global_err/repeat_lib_err4.out0000664000077300007730000000043612231156245022321 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib4 PRIMER_ERROR=Empty sequence in mispriming library repeat_lib4 = primer3-2.3.6/test/primer_global_err/bad_product_sizes_2.out0000664000077300007730000000052012231156245023175 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12 PRIMER_PRODUCT_SIZE_RANGE=75-100 23 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_ERROR=Illegal PRIMER_PRODUCT_SIZE_RANGE value: 23 = primer3-2.3.6/test/primer_global_err/repeat_lib_err4.out20000664000077300007730000000010612231156245022375 0ustar ioanaioana../src/primer3_core: Empty sequence in mispriming library repeat_lib4 primer3-2.3.6/test/primer_global_err/bad_salt_conc.out0000664000077300007730000000051412231156245022027 0ustar ioanaioanaSEQUENCE_ID=bad_salt_conc SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_SALT_MONOVALENT=-1 PRIMER_ERROR=Illegal value for primer salt or dna concentration = primer3-2.3.6/test/primer_global_err/repeat_lib_err3.in0000664000077300007730000000034012231156245022111 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib3 = primer3-2.3.6/test/primer_global_err/bad_min_three_prime_distance.out0000664000077300007730000000126212231156245025103 0ustar ioanaioanaSEQUENCE_ID=error, both general and specific tag in same record PRIMER_PRODUCT_SIZE_RANGE=100-500 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_NUM_RETURN=40 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_ERROR=Both PRIMER_MIN_THREE_PRIME_DISTANCE and PRIMER_{LEFT/RIGHT}_MIN_THREE_PRIME_DISTANCE specified = primer3-2.3.6/test/primer_global_err/bad_dna_conc.out20000664000077300007730000000011012231156245021700 0ustar ioanaioana../src/primer3_core: Illegal value for primer salt or dna concentration primer3-2.3.6/test/primer_global_err/bad_self_any_int.in0000664000077300007730000000042512231156245022334 0ustar ioanaioanaSEQUENCE_ID=bad_self_any_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_SELF_ANY=-1 = primer3-2.3.6/test/primer_global_err/bad_settings2.out20000664000077300007730000000012212231156245022061 0ustar ioanaioana../src/primer3_core: Line 3 must be empty in ./primer_global_err/bad_settings2.in primer3-2.3.6/test/primer_global_err/bad_settings5.in0000664000077300007730000000074512231156245021614 0ustar ioanaioanaPrimer3 File - http://primer3.sourceforge.net P3_FILE_TYPE=settings PRIMER_NO_SUCH_TAG=3 PRIMER_TASK=pick_pcr_primers P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers PRIMER3_MIN_THREE_PRIME_DISTANCE=3 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=300-400 401-500 501-600 601-700 701-850 851-1001 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00 = primer3-2.3.6/test/primer_global_err/bad_num_ns.out20000664000077300007730000000007612231156245021446 0ustar ioanaioana../src/primer3_core: Illegal value for PRIMER_MAX_NS_ACCEPTED primer3-2.3.6/test/primer_global_err/bad_settings6.out20000664000077300007730000000017312231156245022073 0ustar ioanaioana../src/primer3_core: Unknown file type in at line 2 (line2='P3_FILE_TYPE=illegal') in ./primer_global_err/bad_settings6.in primer3-2.3.6/test/primer_global_err/bad_settings1.out20000664000077300007730000000020012231156245022055 0ustar ioanaioana../src/primer3_core: First line must be "Primer3 File - http://primer3.sourceforge.net" in ./primer_global_err/bad_settings1.in primer3-2.3.6/test/primer_global_err/empty_product_size.out0000664000077300007730000000051112231156245023201 0ustar ioanaioanaSEQUENCE_ID=empty_product_size SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_PRODUCT_SIZE_RANGE= PRIMER_ERROR=Empty value for PRIMER_PRODUCT_SIZE_RANGE = primer3-2.3.6/test/primer_global_err/bad_dna_conc.in0000664000077300007730000000040412231156245021423 0ustar ioanaioanaSEQUENCE_ID=bad_dna_conc SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_DNA_CONC=-1 = primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_4.out20000664000077300007730000000012212231156245024217 0ustar ioanaioana../src/primer3_core: Illegal value for primer divalent salt or dNTP concentration primer3-2.3.6/test/primer_global_err/MAX_PRIMER_LENGTH.in0000664000077300007730000000056212231156245021622 0ustar ioanaioanaSEQUENCE_ID=boundary_test_87 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=37 PRIMER_MAX_SIZE=37 PRIMER_OPT_SIZE=37 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=77-77 SEQUENCE_TEMPLATE=NNCACGTCATCATATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATATCTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (testing hard limit MAX_PRIMER_LENGTH) = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err1.in0000664000077300007730000000040212231156245022760 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib1 PRIMER_PICK_INTERNAL_OLIGO=1 = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err2.out0000664000077300007730000000047712231156245023176 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib2 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_ERROR=Empty internal oligo mishyb library repeat_lib2 = primer3-2.3.6/test/primer_global_err/gc_clamp_min_size.in0000664000077300007730000000043412231156245022516 0ustar ioanaioanaSEQUENCE_ID=gc_clamp_min_size SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_GC_CLAMP=19 PRIMER_MIN_SIZE=18 = primer3-2.3.6/test/primer_global_err/mishyb_obj_fn.out0000664000077300007730000000064712231156245022073 0ustar ioanaioanaSEQUENCE_ID=mishyb_lib_not_defined PRIMER_INTERNAL_WT_LIBRARY_MISHYB=1 PRIMER_TASK=pick_pcr_primers_and_hyb_probe SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT PRIMER_ERROR=Internal oligo mispriming score is part of objective function while mishyb library is not defined = primer3-2.3.6/test/primer_global_err/bad_product_sizes_4.out20000664000077300007730000000010512231156245023260 0ustar ioanaioana../src/primer3_core: Illegal PRIMER_PRODUCT_SIZE_RANGE value: 75,100 primer3-2.3.6/test/primer_global_err/bad_settings5.out20000664000077300007730000000014312231156245022067 0ustar ioanaioanaUnrecognized tag: PRIMER_NO_SUCH_TAG=3 ../src/primer3_core: Unrecognized tag: PRIMER_NO_SUCH_TAG=3 primer3-2.3.6/test/primer_global_err/repeat_lib_int_err6.in0000664000077300007730000000040212231156245022765 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib6 PRIMER_PICK_INTERNAL_OLIGO=1 = primer3-2.3.6/test/primer_global_err/repeat_lib_err6.out20000664000077300007730000000010612231156245022377 0ustar ioanaioana../src/primer3_core: Illegal weight in mispriming library repeat_lib6 primer3-2.3.6/test/primer_global_err/must_match_length_7.in0000664000077300007730000000044512231156245023011 0ustar ioanaioanaSEQUENCE_ID=must_match_length_7 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=NNNNNN = primer3-2.3.6/test/primer_global_err/must_match_length_2.out20000664000077300007730000000011212231156245023256 0ustar ioanaioana../src/primer3_core: PRIMER_MUST_MATCH_THREE_PRIME must have 5 characters primer3-2.3.6/test/primer_global_err/bad_self_any.out20000664000077300007730000000011312231156245021737 0ustar ioanaioana../src/primer3_core: Illegal value for primer complementarity restrictions primer3-2.3.6/test/primer_global_err/bad_float_3.out20000664000077300007730000000007112231156245021471 0ustar ioanaioana../src/primer3_core: Illegal PRIMER_MAX_SELF_END value: primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_2.out0000664000077300007730000000073412231156245024144 0ustar ioanaioanaPRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_ID=oligotm_io_2 SEQUENCE_TEMPLATE=CGTCGGTGCGGAAACTTATCGGGTAAAAAGGTTTCTTTAGTTTGGTGTCGATTTTGTCGGTGCCGGTGGCGCAGAACCATTTTACCGAACCGTTTTGACGCTTGGCCCACAGGGAGAGTTTTTTGCCTTGGATTTCTTTG PRIMER_INTERNAL_SALT_DIVALENT=1 PRIMER_INTERNAL_DNTP_CONC=-1 P3_COMMENT=Should fail PRIMER_ERROR=Illegal value for internal oligo divalent salt or dNTP concentration = primer3-2.3.6/test/primer_global_err/repeat_lib_err1.out20000664000077300007730000000012612231156245022374 0ustar ioanaioana../src/primer3_core: Missing id line (expected '>') in mispriming library repeat_lib1 primer3-2.3.6/test/primer_global_err/bad_product_sizes_1.in0000664000077300007730000000042712231156245023001 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12 PRIMER_PRODUCT_SIZE_RANGE=75-100-23 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 = primer3-2.3.6/test/primer_global_err/bad_first_base_index.in0000664000077300007730000000043212231156245023170 0ustar ioanaioanaSEQUENCE_ID=bad_first_base_index SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_FIRST_BASE_INDEX=-1000005 = primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_3.out0000664000077300007730000000054512231156245024145 0ustar ioanaioanaSEQUENCE_ID=oligotm_1 PRIMER_MAX_TM=100 PRIMER_MIN_TM=0 PRIMER_SALT_DIVALENT=-1 PRIMER_DNTP_CONC=1 P3_COMMENT=Should fail SEQUENCE_TEMPLATE=GGCGAGCTGCATCGCGTATTCCATACTTTCCCTGCTGCGGACTTCGCCGATGCAGACCACGTCGGGGGATCAGGTGTGTATCGAGGTGAACATTGCGGGCGAGGCGGTGAAGCACGGTGTCGCGCCCGAAGAAGCAGTCG PRIMER_ERROR=Illegal value for primer divalent salt or dNTP concentration = primer3-2.3.6/test/primer_global_err/seq_quality3.in0000664000077300007730000000117112231156245021476 0ustar ioanaioanaSEQUENCE_ID=quality_1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=20 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_QUALITY_RANGE_MIN=30 = primer3-2.3.6/test/primer_global_err/junction_overlap_min_size.out20000664000077300007730000000011612231156245024612 0ustar ioanaioana../src/primer3_core: Illegal value for PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION primer3-2.3.6/test/primer_global_err/mispr_obj_fn.out0000664000077300007730000000055212231156245021725 0ustar ioanaioanaSEQUENCE_ID=mispr_lib_not_defined PRIMER_WT_LIBRARY_MISPRIMING=1 SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT PRIMER_ERROR=Mispriming score is part of objective function, but mispriming library is not defined = primer3-2.3.6/test/primer_global_err/opt_min_size_int.in0000664000077300007730000000051112231156245022421 0ustar ioanaioanaSEQUENCE_ID=opt_min_size_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_OPT_SIZE=15 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_INTERNAL_MIN_SIZE=18 = primer3-2.3.6/test/primer_global_err/max_size_min_product.in0000664000077300007730000000053712231156245023302 0ustar ioanaioanaSEQUENCE_ID=max_size_min_product SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=20 PRIMER_MIN_SIZE=18 PRIMER_PRODUCT_SIZE_RANGE=24-50 50-75 75-100 = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err1.out0000664000077300007730000000053312231156245023166 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_ERROR=Missing id line (expected '>') in internal oligo mishyb library repeat_lib1 = primer3-2.3.6/test/primer_global_err/bad_float_1.out20000664000077300007730000000011312231156245021464 0ustar ioanaioana../src/primer3_core: Illegal value for primer complementarity restrictions primer3-2.3.6/test/primer_global_err/empty_product_size.out20000664000077300007730000000007712231156245023272 0ustar ioanaioana../src/primer3_core: Empty value for PRIMER_PRODUCT_SIZE_RANGE primer3-2.3.6/test/primer_global_err/bad_float_2.in0000664000077300007730000000043012231156245021204 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12.0.4 PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 = primer3-2.3.6/test/primer_global_err/junction_overlap_max_size.out20000664000077300007730000000012212231156245024611 0ustar ioanaioana../src/primer3_core: PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION > PRIMER_MAX_SIZE / 2 primer3-2.3.6/test/primer_global_err/too_many_product_sizes.out0000664000077300007730000000323512231156245024061 0ustar ioanaioanaSEQUENCE_ID=global_parameter_error_00 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (testing number of product size ranges) PRIMER_ERROR=Too many values for PRIMER_PRODUCT_SIZE_RANGE = primer3-2.3.6/test/primer_global_err/bad_float_4.in0000664000077300007730000000042612231156245021213 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12 PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_INTERNAL_OLIGO=1.0 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 = primer3-2.3.6/test/primer_global_err/bad_self_any.out0000664000077300007730000000051312231156245021661 0ustar ioanaioanaSEQUENCE_ID=bad_self_any SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_SELF_ANY=-1 PRIMER_ERROR=Illegal value for primer complementarity restrictions = primer3-2.3.6/test/primer_global_err/seq_quality4.in0000664000077300007730000000117112231156245021477 0ustar ioanaioanaSEQUENCE_ID=quality_1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=20 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_QUALITY_RANGE_MAX=10 = primer3-2.3.6/test/primer_global_err/bad_product_sizes_3.out20000664000077300007730000000010212231156245023254 0ustar ioanaioana../src/primer3_core: Illegal PRIMER_PRODUCT_SIZE_RANGE value: ,23 primer3-2.3.6/test/primer_global_err/bad_self_end.out20000664000077300007730000000011312231156245021716 0ustar ioanaioana../src/primer3_core: Illegal value for primer complementarity restrictions primer3-2.3.6/test/primer_global_err/bad_gc_int_3.out0000664000077300007730000000053512231156245021552 0ustar ioanaioanaSEQUENCE_ID=bad_gc_int_3 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_GC=60 PRIMER_INTERNAL_MIN_GC=70 PRIMER_ERROR=Illegal value for PRIMER_INTERNAL_OLIGO_GC = primer3-2.3.6/test/primer_global_err/seq_quality1.out20000664000077300007730000000010312231156245021751 0ustar ioanaioana../src/primer3_core: PRIMER_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN primer3-2.3.6/test/primer_global_err/bad_product_sizes_2.in0000664000077300007730000000042712231156245023002 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12 PRIMER_PRODUCT_SIZE_RANGE=75-100 23 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 = primer3-2.3.6/test/primer_global_err/must_match_length_1.out0000664000077300007730000000053312231156245023202 0ustar ioanaioanaSEQUENCE_ID=must_match_length_1 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_FIVE_PRIME=NNNN PRIMER_ERROR=PRIMER_MUST_MATCH_FIVE_PRIME must have 5 characters = primer3-2.3.6/test/primer_global_err/MAX_PRIMER_LENGTH.out20000664000077300007730000000010412231156245022075 0ustar ioanaioana../src/primer3_core: PRIMER_MAX_SIZE exceeds built-in maximum of 36 primer3-2.3.6/test/primer_global_err/bad_product_sizes_1.out20000664000077300007730000000010212231156245023252 0ustar ioanaioana../src/primer3_core: Illegal PRIMER_PRODUCT_SIZE_RANGE value: -23 primer3-2.3.6/test/primer_global_err/bad_float_1.out0000664000077300007730000000053212231156245021407 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=-12.0 PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_ERROR=Illegal value for primer complementarity restrictions = primer3-2.3.6/test/primer_global_err/seq_quality3.out0000664000077300007730000000127512231156245021704 0ustar ioanaioanaSEQUENCE_ID=quality_1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=20 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_QUALITY_RANGE_MIN=30 PRIMER_ERROR=PRIMER_INTERNAL_MIN_QUALITY < PRIMER_QUALITY_RANGE_MIN = primer3-2.3.6/test/primer_global_err/no_equal.out20000664000077300007730000000031112231156245021134 0ustar ioanaioana../src/primer3_core: Input line with no '=': AGTCGATCGTAGCTAGCTAGCTAGCTGACTGATGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTGATCGTAGCTAGCTAGCTGACTGATCATCTAGCTAGCTGATCATCGTAGCTAGCTCTATTTTATCGTAGCTAGCTATCATCTAGCTAGC primer3-2.3.6/test/primer_global_err/repeat_lib_err.out20000664000077300007730000000010012231156245022303 0ustar ioanaioana../src/primer3_core: Cannot open mispriming library repeat_file primer3-2.3.6/test/primer_global_err/bad_float_2.out20000664000077300007730000000007712231156245021476 0ustar ioanaioana../src/primer3_core: Illegal PRIMER_MAX_SELF_END value: 12.0.4 primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_2.out20000664000077300007730000000013212231156245024216 0ustar ioanaioana../src/primer3_core: Illegal value for internal oligo divalent salt or dNTP concentration primer3-2.3.6/test/primer_global_err/repeat_lib_err3.out20000664000077300007730000000010612231156245022374 0ustar ioanaioana../src/primer3_core: Empty sequence in mispriming library repeat_lib3 primer3-2.3.6/test/primer_global_err/repeat_lib_int_err4.in0000664000077300007730000000040212231156245022763 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib4 PRIMER_PICK_INTERNAL_OLIGO=1 = primer3-2.3.6/test/primer_global_err/bad_num_ns_int.out0000664000077300007730000000052512231156245022235 0ustar ioanaioanaSEQUENCE_ID=bad_num_ns_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_NS_ACCEPTED=-1 PRIMER_ERROR=Illegal value for PRIMER_INTERNAL_MAX_NS_ACCEPTED = primer3-2.3.6/test/primer_global_err/must_match_length_2.in0000664000077300007730000000043312231156245023001 0ustar ioanaioanaSEQUENCE_ID=must_match_length_2 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_THREE_PRIME=NNNN = primer3-2.3.6/test/primer_global_err/no_equal.out0000664000077300007730000000056612231156245021066 0ustar ioanaioanaSEQUENCE_ID=without_eq AGTCGATCGTAGCTAGCTAGCTAGCTGACTGATGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTGATCGTAGCTAGCTAGCTGACTGATCATCTAGCTAGCTGATCATCGTAGCTAGCTCTATTTTATCGTAGCTAGCTATCATCTAGCTAGC PRIMER_ERROR=Input line with no '=': AGTCGATCGTAGCTAGCTAGCTAGCTGACTGATGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTGATCGTAGCTAGCTAGCTGACTGATCATCTAGCTAGCTGATCATCGTAGCTAGCTCTATTTTATCGTAGCTAGCTATCATCTAGCTAGC = primer3-2.3.6/test/primer_global_err/zero_num_return.in0000664000077300007730000000150512231156245022311 0ustar ioanaioanaPRIMER_NUM_RETURN=0 SEQUENCE_ID=14.RHAP34FF2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCATTTGGAAAGTCAACCTTCAAGGCGTGTGTTCTTCCTCCTTGGCACCTTGAGGAGGCAGCAATGACATTTTGACAAATGCCTCAAGTTAGGTAGGTGCTCTATAGCAAAGAGCCAATACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGTACGAATGTGTGTAATGAAAGGGGAATATTCTGGCTTAGCTTACACATTAAGTTCATTCAAGGTCATTCAATAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,248 SEQUENCE_TARGET=141,44 = SEQUENCE_ID=15.RHAP34FF3.seq SEQUENCE_TEMPLATE=NNNNNNNNCACTGCCTGTCTCTGCTCTTTACTGAGAACAGTCAAGGGGACAGTATTGTTATCTCCACATATTTCACACTTTTCTTAACCCTTTCCTTAAGATTTTATCTCCTCCATTTTNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGGCCCNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTGCTGGGCTCTTAAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=8,193 SEQUENCE_TARGET=119,24 SEQUENCE_TARGET=154,25 = primer3-2.3.6/test/primer_global_err/bad_gc_int_3.out20000664000077300007730000000010012231156245021620 0ustar ioanaioana../src/primer3_core: Illegal value for PRIMER_INTERNAL_OLIGO_GC primer3-2.3.6/test/primer_global_err/io_gc_content_obj_fn.in0000664000077300007730000000156112231156245023205 0ustar ioanaioanaSEQUENCE_ID=io_gc_content PRIMER_WT_SEQ_QUAL=0 PRIMER_INTERNAL_WT_SEQ_QUAL=0 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_GC=40 PRIMER_INTERNAL_MAX_GC=60 PRIMER_INTERNAL_WT_GC_PERCENT_GT=1 PRIMER_INTERNAL_WT_GC_PERCENT_LT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PAIR_WT_IO_PENALTY=1 PRIMER_PAIR_WT_PR_PENALTY=0 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG = primer3-2.3.6/test/primer_global_err/junction_overlap_min_size.in0000664000077300007730000000452312231156245024335 0ustar ioanaioanaSEQUENCE_ID=Medicago Lyk3 mRNA INTRON_OVERLAP error small SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 SEQUENCE_OVERLAP_JUNCTION_LIST=10 PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION=0 = primer3-2.3.6/test/primer_global_err/bad_settings2.out0000664000077300007730000000011412231156245022000 0ustar ioanaioanaPRIMER_ERROR=Line 3 must be empty in ./primer_global_err/bad_settings2.in = primer3-2.3.6/test/primer_global_err/bad_gc_int_2.in0000664000077300007730000000041412231156245021344 0ustar ioanaioanaSEQUENCE_ID=bad_gc_int_2 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MIN_GC=101 = primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_4.out0000664000077300007730000000055012231156245024142 0ustar ioanaioanaPRIMER_MAX_TM=100 PRIMER_MIN_TM=0 SEQUENCE_ID=oligotm_2 PRIMER_SALT_DIVALENT=1 PRIMER_DNTP_CONC=-1 P3_COMMENT=Should succeed SEQUENCE_TEMPLATE=ACAAGATAATGTTTTGAACATTGTCCTGTTGGTTTCTTTGAAGCAGACCAGAAGTTAAAAAGTTAGAGATCCATAGTGGGTGACAGTCCCGTATTCGAAATCTCAACAGCGGTACTAAGCGTACGAAAAGTAGGGCGGGG PRIMER_ERROR=Illegal value for primer divalent salt or dNTP concentration = primer3-2.3.6/test/primer_global_err/zero_min_size.in0000664000077300007730000000017612231156245021733 0ustar ioanaioanaSEQUENCE_ID=zero min size SEQUENCE_TEMPLATE=aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa PRIMER_MIN_SIZE=0 = primer3-2.3.6/test/primer_global_err/empty_1.in0000664000077300007730000000000012231156245020417 0ustar ioanaioanaprimer3-2.3.6/test/primer_global_err/repeat_lib_err4.in0000664000077300007730000000034012231156245022112 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib4 = primer3-2.3.6/test/primer_global_err/bad_settings3.out0000664000077300007730000000017212231156245022005 0ustar ioanaioanaPRIMER_ERROR=First line must be "Primer3 File - http://primer3.sourceforge.net" in ./primer_global_err/bad_settings3.in = primer3-2.3.6/test/primer_global_err/must_match_length_0.out0000664000077300007730000000052712231156245023204 0ustar ioanaioanaSEQUENCE_ID=must_match_length_0 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_FIVE_PRIME= PRIMER_ERROR=PRIMER_MUST_MATCH_FIVE_PRIME must have 5 characters = primer3-2.3.6/test/primer_global_err/bad_settings2.in0000664000077300007730000000075112231156245021606 0ustar ioanaioanaPrimer3 File - http://primer3.sourceforge.net P3_FILE_TYPE=settings PRIMER_FIRST_BASE_INDEX=1 PRIMER_TASK=pick_pcr_primers P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers PRIMER3_MIN_THREE_PRIME_DISTANCE=3 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=300-400 401-500 501-600 601-700 701-850 851-1001 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00 = primer3-2.3.6/test/primer_global_err/io_obj_fn.in0000664000077300007730000000037612231156245021005 0ustar ioanaioanaSEQUENCE_ID=io_not_required PRIMER_PAIR_WT_IO_PENALTY=1 SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT = primer3-2.3.6/test/primer_global_err/max_size_min_product.out0000664000077300007730000000063412231156245023501 0ustar ioanaioanaSEQUENCE_ID=max_size_min_product SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=20 PRIMER_MIN_SIZE=18 PRIMER_PRODUCT_SIZE_RANGE=24-50 50-75 75-100 PRIMER_ERROR=PRIMER_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE = primer3-2.3.6/test/primer_global_err/bad_settings4.out0000664000077300007730000000014112231156245022002 0ustar ioanaioanaPRIMER_ERROR=Unknown file type in at line 2 (line2='') in ./primer_global_err/bad_settings4.in = primer3-2.3.6/test/primer_global_err/repeat_lib_err2.out0000664000077300007730000000042212231156245022312 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib2 PRIMER_ERROR=Empty mispriming library repeat_lib2 = primer3-2.3.6/test/primer_global_err/zero_min_size.out0000664000077300007730000000035112231156245022127 0ustar ioanaioanaSEQUENCE_ID=zero min size SEQUENCE_TEMPLATE=aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa PRIMER_MIN_SIZE=0 PRIMER_ERROR=PRIMER_MIN_SIZE must be >= 1; SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = primer3-2.3.6/test/primer_global_err/junction_overlap_max_size.in0000664000077300007730000000455012231156245024337 0ustar ioanaioanaSEQUENCE_ID=Medicago Lyk3 mRNA INTRON_OVERLAP error big SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 SEQUENCE_OVERLAP_JUNCTION_LIST=10 15 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=14 PRIMER_MAX_SIZE=27 = primer3-2.3.6/test/primer_global_err/no_terminal_equal.out20000664000077300007730000000007012231156245023031 0ustar ioanaioana../src/primer3_core: Final record not terminated by '=' primer3-2.3.6/test/primer_global_err/bad_settings4.out20000664000077300007730000000014712231156245022072 0ustar ioanaioana../src/primer3_core: Unknown file type in at line 2 (line2='') in ./primer_global_err/bad_settings4.in primer3-2.3.6/test/primer_global_err/product_tm_obj_fn.in0000664000077300007730000000041012231156245022543 0ustar ioanaioanaSEQUENCE_ID=product_tm_opt_not_def PRIMER_PAIR_WT_PRODUCT_TM_LT=1 SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT = primer3-2.3.6/test/primer_global_err/seq_quality2.in0000664000077300007730000000112312231156245021472 0ustar ioanaioanaSEQUENCE_ID=quality_1 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_MIN_QUALITY=20 PRIMER_QUALITY_RANGE_MAX=10 = primer3-2.3.6/test/primer_global_err/product_tm_obj_fn.out20000664000077300007730000000016012231156245023030 0ustar ioanaioana../src/primer3_core: Product temperature is part of objective function while optimum temperature is not defined primer3-2.3.6/test/primer_global_err/must_match_length_6.out0000664000077300007730000000053712231156245023213 0ustar ioanaioanaSEQUENCE_ID=must_match_length_6 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_THREE_PRIME=NNNNNN PRIMER_ERROR=PRIMER_MUST_MATCH_THREE_PRIME must have 5 characters = primer3-2.3.6/test/primer_global_err/must_match_length_7.out0000664000077300007730000000055712231156245023216 0ustar ioanaioanaSEQUENCE_ID=must_match_length_7 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=NNNNNN PRIMER_ERROR=PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME must have 5 characters = primer3-2.3.6/test/primer_global_err/bad_gc_3.in0000664000077300007730000000041712231156245020476 0ustar ioanaioanaSEQUENCE_ID=bad_gc_3 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_GC=60 PRIMER_MIN_GC=70 = primer3-2.3.6/test/primer_global_err/must_match_length_10.out20000664000077300007730000000010612231156245023340 0ustar ioanaioana../src/primer3_core: Illegal values for PRIMER_MUST_MATCH_THREE_PRIME primer3-2.3.6/test/primer_global_err/bad_gc_int_1.out0000664000077300007730000000050312231156245021543 0ustar ioanaioanaSEQUENCE_ID=bad_gc_int_1 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_GC=-2 PRIMER_ERROR=Illegal value for PRIMER_INTERNAL_OLIGO_GC = primer3-2.3.6/test/primer_global_err/must_match_length_1.out20000664000077300007730000000011112231156245023254 0ustar ioanaioana../src/primer3_core: PRIMER_MUST_MATCH_FIVE_PRIME must have 5 characters primer3-2.3.6/test/primer_global_err/mishyb_obj_fn.out20000664000077300007730000000016712231156245022152 0ustar ioanaioana../src/primer3_core: Internal oligo mispriming score is part of objective function while mishyb library is not defined primer3-2.3.6/test/primer_global_err/bad_product_sizes_3.in0000664000077300007730000000042712231156245023003 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12 PRIMER_PRODUCT_SIZE_RANGE=75-100,23 PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 = primer3-2.3.6/test/primer_global_err/pair_mispr.in0000664000077300007730000000040412231156245021216 0ustar ioanaioanaSEQUENCE_ID=mispr_library PRIMER_PAIR_WT_LIBRARY_MISPRIMING=1 SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT = primer3-2.3.6/test/primer_global_err/min_opt_tm_int.in0000664000077300007730000000050112231156245022066 0ustar ioanaioanaSEQUENCE_ID=min_opt_tm_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_TM=65 PRIMER_INTERNAL_OPT_TM=60 PRIMER_INTERNAL_MIN_TM=62 = primer3-2.3.6/test/primer_global_err/bad_gc_int_1.in0000664000077300007730000000041312231156245021342 0ustar ioanaioanaSEQUENCE_ID=bad_gc_int_1 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_GC=-2 = primer3-2.3.6/test/primer_global_err/must_match_length_6.out20000664000077300007730000000011212231156245023262 0ustar ioanaioana../src/primer3_core: PRIMER_MUST_MATCH_THREE_PRIME must have 5 characters primer3-2.3.6/test/primer_global_err/seq_quality4.out20000664000077300007730000000011412231156245021756 0ustar ioanaioana../src/primer3_core: PRIMER_INTERNAL_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX primer3-2.3.6/test/primer_global_err/bad_self_any_int.out20000664000077300007730000000012312231156245022612 0ustar ioanaioana../src/primer3_core: Illegal value for internal oligo complementarity restrictions primer3-2.3.6/test/primer_global_err/repeat_lib_int_err2.out20000664000077300007730000000010512231156245023244 0ustar ioanaioana../src/primer3_core: Empty internal oligo mishyb library repeat_lib2 primer3-2.3.6/test/primer_global_err/bad_salt_conc.in0000664000077300007730000000041412231156245021625 0ustar ioanaioanaSEQUENCE_ID=bad_salt_conc SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_SALT_MONOVALENT=-1 = primer3-2.3.6/test/primer_global_err/bad_gc_int_2.out20000664000077300007730000000010012231156245021617 0ustar ioanaioana../src/primer3_core: Illegal value for PRIMER_INTERNAL_OLIGO_GC primer3-2.3.6/test/primer_global_err/zero_num_return.out20000664000077300007730000000005312231156245022571 0ustar ioanaioana../src/primer3_core: PRIMER_NUM_RETURN < 1 primer3-2.3.6/test/primer_global_err/bad_gc_3.out0000664000077300007730000000051612231156245020677 0ustar ioanaioanaSEQUENCE_ID=bad_gc_3 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_GC=60 PRIMER_MIN_GC=70 PRIMER_ERROR=Illegal value for PRIMER_MAX_GC and PRIMER_MIN_GC = primer3-2.3.6/test/primer_global_err/seq_quality2.out20000664000077300007730000000010312231156245021752 0ustar ioanaioana../src/primer3_core: PRIMER_MIN_QUALITY > PRIMER_QUALITY_RANGE_MAX primer3-2.3.6/test/primer_global_err/bad_self_end_int.out0000664000077300007730000000054012231156245022512 0ustar ioanaioanaSEQUENCE_ID=bad_self_end_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_SELF_END=-1 PRIMER_ERROR=Illegal value for internal oligo complementarity restrictions = primer3-2.3.6/test/primer_global_err/max_size_min_product_int.in0000664000077300007730000000067412231156245024156 0ustar ioanaioanaSEQUENCE_ID=max_size_min_product_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_SIZE=24 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_PRODUCT_SIZE_RANGE=24-50 50-75 75-100 = primer3-2.3.6/test/primer_global_err/bad_settings3.out20000664000077300007730000000020012231156245022057 0ustar ioanaioana../src/primer3_core: First line must be "Primer3 File - http://primer3.sourceforge.net" in ./primer_global_err/bad_settings3.in primer3-2.3.6/test/primer_global_err/must_match_length_5.out0000664000077300007730000000053512231156245023210 0ustar ioanaioanaSEQUENCE_ID=must_match_length_5 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_FIVE_PRIME=NNNNNN PRIMER_ERROR=PRIMER_MUST_MATCH_FIVE_PRIME must have 5 characters = primer3-2.3.6/test/primer_global_err/max_size_min_product_int.out20000664000077300007730000000011612231156245024430 0ustar ioanaioana../src/primer3_core: PRIMER_INTERNAL_MAX_SIZE > min PRIMER_PRODUCT_SIZE_RANGE primer3-2.3.6/test/primer_global_err/opt_max_size_int.in0000664000077300007730000000051112231156245022423 0ustar ioanaioanaSEQUENCE_ID=opt_max_size_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_INTERNAL_MIN_SIZE=18 = primer3-2.3.6/test/primer_global_err/repeat_lib_err2.out20000664000077300007730000000007212231156245022375 0ustar ioanaioana../src/primer3_core: Empty mispriming library repeat_lib2 primer3-2.3.6/test/primer_global_err/max_opt_tm_int.in0000664000077300007730000000050112231156245022070 0ustar ioanaioanaSEQUENCE_ID=max_opt_tm_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_TM=65 PRIMER_INTERNAL_OPT_TM=66 PRIMER_INTERNAL_MIN_TM=62 = primer3-2.3.6/test/primer_global_err/max_opt_tm_int.out0000664000077300007730000000062212231156245022275 0ustar ioanaioanaSEQUENCE_ID=max_opt_tm_int SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MAX_TM=65 PRIMER_INTERNAL_OPT_TM=66 PRIMER_INTERNAL_MIN_TM=62 PRIMER_ERROR=Optimum internal oligo Tm lower than minimum or higher than maximum = primer3-2.3.6/test/primer_global_err/junction_overlap_max_size.out0000664000077300007730000000466212231156245024544 0ustar ioanaioanaSEQUENCE_ID=Medicago Lyk3 mRNA INTRON_OVERLAP error big SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 SEQUENCE_OVERLAP_JUNCTION_LIST=10 15 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=14 PRIMER_MAX_SIZE=27 PRIMER_ERROR=PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION > PRIMER_MAX_SIZE / 2 = primer3-2.3.6/test/primer_global_err/repeat_lib_err1.out0000664000077300007730000000045612231156245022320 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib1 PRIMER_ERROR=Missing id line (expected '>') in mispriming library repeat_lib1 = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err6.out20000664000077300007730000000012112231156245023246 0ustar ioanaioana../src/primer3_core: Illegal weight in internal oligo mishyb library repeat_lib6 primer3-2.3.6/test/primer_global_err/io_gc_content_obj_fn.out0000664000077300007730000000173112231156245023405 0ustar ioanaioanaSEQUENCE_ID=io_gc_content PRIMER_WT_SEQ_QUAL=0 PRIMER_INTERNAL_WT_SEQ_QUAL=0 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_GC=40 PRIMER_INTERNAL_MAX_GC=60 PRIMER_INTERNAL_WT_GC_PERCENT_GT=1 PRIMER_INTERNAL_WT_GC_PERCENT_LT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PAIR_WT_IO_PENALTY=1 PRIMER_PAIR_WT_PR_PENALTY=0 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG PRIMER_ERROR=Hyb probe GC content is part of objective function while optimum gc_content is not defined = primer3-2.3.6/test/primer_global_err/must_match_length_4.out0000664000077300007730000000055712231156245023213 0ustar ioanaioanaSEQUENCE_ID=must_match_length_4 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=NNNN PRIMER_ERROR=PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME must have 5 characters = primer3-2.3.6/test/primer_global_err/zero_num_return.out0000664000077300007730000000071012231156245022507 0ustar ioanaioanaPRIMER_NUM_RETURN=0 SEQUENCE_ID=14.RHAP34FF2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCATTTGGAAAGTCAACCTTCAAGGCGTGTGTTCTTCCTCCTTGGCACCTTGAGGAGGCAGCAATGACATTTTGACAAATGCCTCAAGTTAGGTAGGTGCTCTATAGCAAAGAGCCAATACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGTACGAATGTGTGTAATGAAAGGGGAATATTCTGGCTTAGCTTACACATTAAGTTCATTCAAGGTCATTCAATAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,248 SEQUENCE_TARGET=141,44 PRIMER_ERROR=PRIMER_NUM_RETURN < 1 = primer3-2.3.6/test/primer_global_err/empty_1.out20000664000077300007730000000424512231156245020721 0ustar ioanaioana Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 2009,2010,2011,2012 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of the primer3 suite and libraries. The primer3 suite and libraries are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this software (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. USAGE: ../src/primer3_core [-format_output] [-default_version=1|-default_version=2] [-io_version=4] [-p3_settings_file=] [-echo_settings_file] [-strict_tags] [-output=] [-error=] [input_file] This is primer3 (libprimer3 release 2.3.5) Input can also be provided on standard input. For example: $ primer3_core < my_input_file primer3-2.3.6/test/primer_global_err/bad_gc_int_1.out20000664000077300007730000000010012231156245021616 0ustar ioanaioana../src/primer3_core: Illegal value for PRIMER_INTERNAL_OLIGO_GC primer3-2.3.6/test/primer_global_err/must_match_length_9.out0000664000077300007730000000053212231156245023211 0ustar ioanaioanaSEQUENCE_ID=must_match_length_9 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_THREE_PRIME=NNZNN PRIMER_ERROR=Illegal values for PRIMER_MUST_MATCH_THREE_PRIME = primer3-2.3.6/test/primer_global_err/must_match_length_10.out0000664000077300007730000000053312231156245023262 0ustar ioanaioanaSEQUENCE_ID=must_match_length_10 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_THREE_PRIME=NQNNN PRIMER_ERROR=Illegal values for PRIMER_MUST_MATCH_THREE_PRIME = primer3-2.3.6/test/primer_global_err/repeat_lib_err3.out0000664000077300007730000000043612231156245022320 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_MISPRIMING_LIBRARY=repeat_lib3 PRIMER_ERROR=Empty sequence in mispriming library repeat_lib3 = primer3-2.3.6/test/primer_global_err/incorrect_task.in0000664000077300007730000000042112231156245022062 0ustar ioanaioanaSEQUENCE_ID=incorrect_task PRIMER_TASK=pick_pcr_primers_and_internal_oligo SEQUENCE_TEMPLATE=CAGCTGATCGATCGATCGATCGTAGCTAGCTAGCTAGCTAGCTGATCGATCGATGCTAGCTAGCTAGCTAGTCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCTAGCTAGCTGACTGATCGATCGATGCTAGCTAGCTAGCTAGCTGATCGATCGTAGCTAGCT = primer3-2.3.6/test/primer_global_err/pr_gc_content_obj_fn.out20000664000077300007730000000015512231156245023500 0ustar ioanaioana../src/primer3_core: Primer GC content is part of objective function while optimum gc_content is not defined primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_3.in0000664000077300007730000000043312231156245023740 0ustar ioanaioanaSEQUENCE_ID=oligotm_1 PRIMER_MAX_TM=100 PRIMER_MIN_TM=0 PRIMER_SALT_DIVALENT=-1 PRIMER_DNTP_CONC=1 P3_COMMENT=Should fail SEQUENCE_TEMPLATE=GGCGAGCTGCATCGCGTATTCCATACTTTCCCTGCTGCGGACTTCGCCGATGCAGACCACGTCGGGGGATCAGGTGTGTATCGAGGTGAACATTGCGGGCGAGGCGGTGAAGCACGGTGTCGCGCCCGAAGAAGCAGTCG = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err6.out0000664000077300007730000000051312231156245023171 0ustar ioanaioanaSEQUENCE_ID=seq1 SEQUENCE_TEMPLATE=ATGCTAGCTAGTCGATCGATGCTAGCTAGTCGATCTACTATCATCATGCTAGCATGGGGCATCTGTGGGATGCTGTCGTACTGATCGATGCTAGCTAGCTAGTCGATCGTAGCTATCATCATCTAGTCATCGTAGCTATCATCGTAGC PRIMER_INTERNAL_MISHYB_LIBRARY=repeat_lib6 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_ERROR=Illegal weight in internal oligo mishyb library repeat_lib6 = primer3-2.3.6/test/primer_global_err/must_match_length_1.in0000664000077300007730000000043212231156245022777 0ustar ioanaioanaSEQUENCE_ID=must_match_length_1 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MUST_MATCH_FIVE_PRIME=NNNN = primer3-2.3.6/test/primer_global_err/repeat_lib_int_err3.out20000664000077300007730000000012112231156245023243 0ustar ioanaioana../src/primer3_core: Empty sequence in internal oligo mishyb library repeat_lib3 primer3-2.3.6/test/primer_global_err/empty_1.out0000664000077300007730000000000012231156245020620 0ustar ioanaioanaprimer3-2.3.6/test/primer_global_err/must_match_length_4.out20000664000077300007730000000012312231156245023262 0ustar ioanaioana../src/primer3_core: PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME must have 5 characters primer3-2.3.6/test/primer_global_err/bad_divalent_dntp_conc_1.out0000664000077300007730000000073412231156245024143 0ustar ioanaioanaSEQUENCE_ID=oligotm_io_1 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=GTCGCCCGGCTGCGCGCCTGTATCCACATCCAAGAGCTTCAGTTTCCCGTCTGCCGTGGCGGCACTCAAATTTTCGTCCACCAGGGCAAGCGGCCCGTGCTTCAGTTCGCCGGCAGGATAGGCTTCGGCGTGGATGTAGG PRIMER_INTERNAL_SALT_DIVALENT=-1 PRIMER_INTERNAL_DNTP_CONC=1 P3_COMMENT=Should fail PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_ERROR=Illegal value for internal oligo divalent salt or dNTP concentration = primer3-2.3.6/test/primer_global_err/bad_gc_1.in0000664000077300007730000000037612231156245020500 0ustar ioanaioanaSEQUENCE_ID=bad_gc_1 SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_GC=-2 = primer3-2.3.6/test/primer_global_err/bad_settings1.out0000664000077300007730000000017212231156245022003 0ustar ioanaioanaPRIMER_ERROR=First line must be "Primer3 File - http://primer3.sourceforge.net" in ./primer_global_err/bad_settings1.in = primer3-2.3.6/test/primer_global_err/bad_float_4.out0000664000077300007730000000052112231156245021410 0ustar ioanaioanaSEQUENCE_ID=bad_float SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG PRIMER_MAX_SELF_END=12 PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_INTERNAL_OLIGO=1.0 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_ERROR=Illegal PRIMER_PICK_INTERNAL_OLIGO value: 1.0 = primer3-2.3.6/test/primer_global_err/opt_max_size.in0000664000077300007730000000045212231156245021555 0ustar ioanaioanaSEQUENCE_ID=opt_max_size SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_OPT_SIZE=25 PRIMER_MAX_SIZE=20 PRIMER_MIN_SIZE=18 = primer3-2.3.6/test/primer_global_err/bad_num_ns.out0000664000077300007730000000047712231156245021371 0ustar ioanaioanaSEQUENCE_ID=bad_num_ns SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_NS_ACCEPTED=-1 PRIMER_ERROR=Illegal value for PRIMER_MAX_NS_ACCEPTED = primer3-2.3.6/test/primer_global_err/bad_num_ns.in0000664000077300007730000000041112231156245021154 0ustar ioanaioanaSEQUENCE_ID=bad_num_ns SEQUENCE_TEMPLATE=TAGCTGATGCTAGCTAGCGTGGGCATGCTAGCTAGCTGATGCAGCTAGCTGATCGTAGCTAGCTATCGATCGTAGCTGACTGATCGTAGCTAGCTGATCGATGCTAGCTAGCTAGCTGATCATCTACGTAGCTGACTATCGATGCTACTACTATCGATCGTAGCTAGCTGATCGTAGCTAGCTAGCTGATGCTAC PRIMER_MAX_NS_ACCEPTED=-1 = primer3-2.3.6/test/cmdline_test.pl0000664000077300007730000002705612231156245016056 0ustar ioanaioana # Command line test driver for the primer3_core executable. # # For usage, see the usage statement in the code, below. # # ====================================================================== # (c) Copyright 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008, # 2010,2011,2012 # Whitehead Institute for Biomedical Research, Steve Rozen, # Andreas Untergasser and Helen Skaletsky # All rights reserved. # # This file is part of the primer3 suite. # # The primer3 suite is free software; you can # redistribute them and/or modify them under the terms of the GNU # General Public License as published by the Free Software Foundation; # either version 2 of the License, or (at your option) any later # version. # # This software is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # # You should have received a copy of the GNU General Public License # along with this file (file gpl-2.0.txt in the source distribution); if # not, write to the Free Software Foundation, Inc., 51 Franklin St, # Fifth Floor, Boston, MA 02110-1301 USA # # THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS # "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT # LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR # A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT # OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, # SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT # LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, # DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY # THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT # (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE # OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. # ====================================================================== use warnings 'all'; use strict; use Cwd; use Getopt::Long; use POSIX; # For testing wait / system return value, e.g. WIFSIGNALED, WTERMSIG..... use Config; # To get the number for SIGINT sub perldiff($$); sub runtest($$$$); sub main(); # Call system() with warnings turned off; needed for ActiveState / MS Windows. sub _nowarn_system($); our $def_executable = "../src/primer3_core"; our $exe = '../src/primer3_core'; our $set_files = '../test/'; our ($verbose, $do_valgrind, $winFlag, $fastFlag); our %signo; our $valgrind_format; # Global variable for Errors my $all_ok; main(); sub main() { my %args; # select STDERR; $| = 1; $all_ok = 1; if (defined $Config{sig_name}) { my $i = 0; for my $name (split(' ', $Config{sig_name})) { $signo{$name} = $i; $i++; } } print "\n\nTESTING command line arguments\n\n"; # GetOptions handles various flag abbreviations and formats, # such as -e ../src/primer3_core, --exe ../src/primer3_core, # --exe=.../src/primer3_core if (!GetOptions(\%args, 'valgrind', 'windows', 'fast', 'verbose', 'executable=s', )) { print "Usage: perl cmdline_test.pl \\\n", " [--executable ] [ --valgrind ] [ --verbose ] [--windows] [--fast]\n", "\n", " where defaults to ../src/primer3_core\n"; exit -1; } $exe = $args{'executable'} if defined$ args{'executable'}; $winFlag = defined $args{'windows'}; $verbose = defined $args{'verbose'}; $fastFlag = defined $args{'fast'}; $do_valgrind = $args{'valgrind'}; if ($winFlag && $do_valgrind) { print "$0: Cannot specify both --valgrind and --windows\n"; exit -1; } my $valgrind_exe = "/usr/local/bin/valgrind"; my $log_file_arg_for_valgrind = "--log-file-exactly"; if ($do_valgrind) { if (!-x $valgrind_exe) { warn "Cannot find $valgrind_exe; will try `which valgrind`\n"; $valgrind_exe= `which valgrind`; chomp($valgrind_exe); if (!$valgrind_exe || ! -x $valgrind_exe) { die "Cannot execute $valgrind_exe"; } } # Need to deal with different arguments in # different version of valgrind my $valgrind_version = `$valgrind_exe --version`; if ($valgrind_version =~ /([0-9]+)\.([0-9]+)\./) { if (($1 > 3) || (($1 == 3) && ($2 > 2))) { $log_file_arg_for_valgrind = "--log-file"; } } } $valgrind_format = $valgrind_exe . " --leak-check=yes --show-reachable=yes " . "$log_file_arg_for_valgrind=%s.vg "; if ($winFlag) { $exe = '..\\src\\primer3_core.exe'; $set_files = '..\\test\\'; } my $exit_stat = 0; die "Cannot execute $exe" unless -x $exe; my $valgrind_prefix; my $cmd; # Use primer_check_input and primer_check_output files my $input = 'primer_check' . '_input'; my $output = 'primer_check' . '_output'; # Make sure that needed files are present and readable die "Cannot read $input" unless -r $input; die "Cannot read $output" unless -r $output; # Test 1 : check '-output' and 'input_file' $valgrind_prefix = $do_valgrind ? sprintf $valgrind_format, 'cmd_test1' : ''; $cmd = "$valgrind_prefix$exe -default_version=1 --strict -o cmd_test1.tmp $input"; $exit_stat = runtest(1, $cmd, $output, 0); # Test 2 : check '> output' and '< input_file' $valgrind_prefix = $do_valgrind ? sprintf $valgrind_format, 'cmd_test2' : ''; $cmd = "$valgrind_prefix$exe -default_version=1 -st > cmd_test2.tmp < $input"; $exit_stat = runtest(2, $cmd, $output, 0); # Test 3 : check incorrect flag and '-error_file' $valgrind_prefix = $do_valgrind ? sprintf $valgrind_format, 'cmd_test3' : ''; $cmd = "$valgrind_prefix$exe -flag -err=cmd_test3.tmp < $input"; $exit_stat = runtest(3, $cmd, 'cmd_test3_output', 255); # Test 4 : check nonexistent input file and '2> error_file' $valgrind_prefix = $do_valgrind ? sprintf $valgrind_format, 'cmd_test4' : ''; $cmd = "$valgrind_prefix$exe invalid_input 2> cmd_test4.tmp"; $exit_stat = runtest(4, $cmd, 'cmd_test4_output', 255); # Test 5 : check that io_version=3 fails $valgrind_prefix = $do_valgrind ? sprintf $valgrind_format, 'cmd_test5' : ''; $cmd = "$valgrind_prefix$exe -io_version=3 2> cmd_test5.tmp"; $exit_stat = runtest(5, $cmd, 'cmd_test5_output', 255); # ================================================== # If we were running under valgrind to look for memory-related # errors (reading uninitialized memory, writing off the end of # an array, etc) or leaks, then we look through the valgrind # logs to summarize errors and leaks. if ($do_valgrind) { # Assume this is Unix/Linux environment, so # we have grep. my $r = system "grep ERROR *.vg | grep -v 'ERROR SUMMARY: 0 errors'"; if (!$r) { # !$r because grep returns 0 if something is found, # and if something is found, we have a problem. $exit_stat = -1; } $r = system "grep 'definitely lost' *.vg | grep -v ' 0 bytes'"; if (!$r) { $exit_stat = -1; } $r = system "grep 'possibly lost' *.vg | grep -v ' 0 bytes'"; if (!$r) { $exit_stat = -1; } } print $all_ok ? "Passed all tests - [OK]\n\n\n" : "At least one test failed - [FAILED]\n\n"; exit $exit_stat; } # Usage: runtest(test_number, command, output_file, expected_exit_code) sub runtest($$$$) { my $r; my $exit_stat = 0; my ($i, $cmd, $output, $exit) = @_; print "Test $i... "; $r = _nowarn_system($cmd); if ($winFlag) { $r = $r >> 8; } unless ($r == $exit) { $all_ok = 0; print "incorrect exit code [FAILED]\n"; $exit_stat = -1; } else { $r = perldiff $output, "cmd_test$i.tmp"; if ($r == 0) { print "[OK]\n"; } else { $all_ok = 0; print "[FAILED]\n"; $exit_stat = -1; } } return $exit_stat; } # Usage: perldiff("filename1", "filename2") # Return 0 if no differences are found, # othewise return 1; sub perldiff($$) { my ($f1, $f2) = @_; open F1, $f1 or die "open $f1: $!"; open F2, $f2 or die "open $f2: $!"; my @f1 = ; my @f2 = ; # If different number of lines, return FAIL. if (@f1 != @f2) { print "Different number of lines ($f1 versus $f2)\n"; return 1; } # check for differences on the lines, themselves my $linenumber = 0; my $line_end_diff = 0; # iterate using lines in file1 while (@f1) { $linenumber++; # get the lines from each respective file my $l1 = shift @f1; my $l2 = shift @f2; my $l1_orig = $l1; my $l2_orig = $l2; # Handle the diff in empty_1.{out2,tmp2} due to # different executable names. if ($exe ne $def_executable && $l1 =~/^USAGE:\s+\S+/) { $l1 =~ s/^USAGE:\s+\S+/USAGE: ... /i; $l2 =~ s/^USAGE:\s+\S+/USAGE: ... /i; if ($verbose) { print "removing executable name from\n", "$l1_orig\n$l2_orig\n"; } } # Remove everything up to and including the first # colon, which removes the executable name. # This substitution must follow the USAGE # substitution, above my $regex = "[^:]+:"; my $quoteexe = quotemeta($def_executable); # Edit executable name if ($exe ne $def_executable && ($l1 =~ /$quoteexe/ || $l2 =~ /$quoteexe/)) { $l1 =~ s/$regex//g; $l2 =~ s/$regex//g; if ($verbose) { print "removing : from\n", "$l1_orig\n$l2_orig\n"; } } # Edit release number if ($l1 ne $l2) { if ($l1 =~ /(libprimer3|primer3) release \d+\.\d+\.\d+/ && $l2 =~ /(libprimer3|primer3) release \d+\.\d+\.\d+/) { $l1 =~ s/(libprimer3|primer3) release \d+\.\d+\.\d+//; $l2 =~ s/(libprimer3|primer3) release \d+\.\d+\.\d+//; } } # If this is the tag with the settings file path, replace \ by / to make it # the same on both Linux and Windows if ($l1 =~ /^P3_SETTINGS_FILE_USED/ && $l2 =~ /^P3_SETTINGS_FILE_USED/) { $l1 =~ s/\\/\//g; $l2 =~ s/\\/\//g; } $linenumber++; # Check for difference between two edited lines (line by line) if ($l1 ne $l2) { print "Difference found at line $linenumber:\n< $l1_orig\n> $l2_orig\n"; return 1; } } return 0; } sub _nowarn_system($) { my $cmd = shift; if ($verbose) { print "\n$cmd\n" } no warnings 'all'; my $r = system $cmd; my $r2 = $?; if (!$winFlag && WIFEXITED($r2)) { $r = WEXITSTATUS($r2); if (defined $signo{'INT'}) { if ($r == $signo{'INT'}) { print "\nCommand: $cmd\n"; print "generated exit value $r\n"; print "Presumably caused by catching SIGINT\n"; print "Tests halted\n\n"; print "\nWARNING: not all tests executed ... [FAILED]\n"; exit; } } } if (!$winFlag && WIFSIGNALED($r2)) { my $r2 = WTERMSIG($r2); print "Exited with signal $r2\n"; } $r; } primer3-2.3.6/test/p3_3_prime_0_input0000664000077300007730000001600612231156245016361 0ustar ioanaioanaPRIMER_MIN_THREE_PRIME_DISTANCE=0 PRIMER_NUM_RETURN=2 PRIMER_OPT_SIZE=20 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000 PRIMER_MAX_GC=80.0 PRIMER_MAX_SIZE=25 PRIMER_MAX_TM=63.0 PRIMER_MIN_SIZE=18 PRIMER_MIN_GC=20 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=6 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=6 MARKER_NAME=01.RHAP33FA1.seq SEQUENCE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN INCLUDED_REGION=20,315 TARGET=81,30,CA = MARKER_NAME=01.RHAP34FA1.seq SEQUENCE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT INCLUDED_REGION=19,296 TARGET=134,15,CA TARGET=213,16,AT TARGET=235,16,AT = PRIMER_NUM_RETURN=1 MARKER_NAME=03.RHAP34FA3.seq SEQUENCE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT INCLUDED_REGION=0,278 TARGET=1,28,CA = MARKER_NAME=03.RHAP34FE3.seq SEQUENCE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA INCLUDED_REGION=17,284 TARGET=27,60,AC = MARKER_NAME=04.RHAP33FE4.seq SEQUENCE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC INCLUDED_REGION=18,367 TARGET=146,46,AT TARGET=199,22,TA TARGET=286,13,GT TARGET=300,15,AG = MARKER_NAME=05.RHAP33FA5.seq SEQUENCE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA INCLUDED_REGION=10,369 TARGET=30,44,GT = MARKER_NAME=05.RHAP33FE5.seq SEQUENCE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN INCLUDED_REGION=14,306 TARGET=176,34,CA = PRIMER_NUM_RETURN=40 MARKER_NAME=05.RHAP34FA5.seq SEQUENCE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA INCLUDED_REGION=9,297 TARGET=125,36,AC TARGET=162,24,GA TARGET=191,16,AG TARGET=214,14,GA = MARKER_NAME=06.RHAP34FE6.seq SEQUENCE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA INCLUDED_REGION=8,287 TARGET=61,68,CA = MARKER_NAME=07.RHAP34FE7.seq SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT INCLUDED_REGION=18,283 TARGET=115,29,CT TARGET=145,20,NG TARGET=166,24,CA = MARKER_NAME=08.RHAP34FE8.seq SEQUENCE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG INCLUDED_REGION=20,281 TARGET=119,62,AC = PRIMER_NUM_RETURN=8 MARKER_NAME=09.RHAP33FA9.seq SEQUENCE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG INCLUDED_REGION=0,371 TARGET=151,18,AC TARGET=192,53,GA TARGET=248,16,AG = MARKER_NAME=09.RHAP34FA9.seq SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT INCLUDED_REGION=20,280 TARGET=44,42,CA = MARKER_NAME=10.RHAP34FA10.seq SEQUENCE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN INCLUDED_REGION=12,236 TARGET=155,32,TG = MARKER_NAME=13.RHAP34FB1.seq SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN INCLUDED_REGION=21,244 TARGET=22,16,TA TARGET=57,34,GT = MARKER_NAME=14.RHAP34FB2.seq SEQUENCE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC INCLUDED_REGION=13,303 TARGET=177,33,AC TARGET=254,14,AC = primer3-2.3.6/test/primer_boundary1_input0000664000077300007730000012035312231156245017465 0ustar ioanaioanaPRIMER_FIRST_BASE_INDEX=1 SEQUENCE_ID=boundary_test_00 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 = SEQUENCE_ID=boundary_test_01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-100 = SEQUENCE_ID=boundary_test_02 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 = SEQUENCE_ID=boundary_test_03 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="56-100" P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_04 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="50-54" P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_05 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MIN_SIZE=24 PRIMER_MAX_SIZE=24 PRIMER_OPT_SIZE=24 = SEQUENCE_ID=boundary_test_06 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MIN_SIZE=26 PRIMER_MAX_SIZE=26 PRIMER_OPT_SIZE=26 P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_07 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 SEQUENCE_INCLUDED_REGION=3,55 = SEQUENCE_ID=boundary_test_08 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="50-100" PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 SEQUENCE_INCLUDED_REGION=4,55 P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_09 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="50-100" SEQUENCE_INCLUDED_REGION=3,54 P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_10 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_12 PRIMER_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_13 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_14 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=.0001 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_15 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=20 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_16 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=.0001 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_17 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_18 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=4,55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_19 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,54 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_20 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE="54-55" SEQUENCE_INCLUDED_REGION=3,54 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_21 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_ID=boundary_test_22 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,6 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_23 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,6 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_24 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=29,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,1 SEQUENCE_TARGET=29,4 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_26 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,1 SEQUENCE_TARGET=28,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_27 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,1 SEQUENCE_TARGET=29,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_28 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=29,1 SEQUENCE_TARGET=30,4 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_29 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_30 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=29,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_31 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=1,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_32 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_33 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,6 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_34 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,6 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_35 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_36 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 SEQUENCE_EXCLUDED_REGION=29,4 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_37 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,1 SEQUENCE_EXCLUDED_REGION=28,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_37 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 SEQUENCE_EXCLUDED_REGION=29,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_38 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 SEQUENCE_EXCLUDED_REGION=30,4 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_39 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_40 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_41 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=11,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_42 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_43 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=9.99 PRIMER_PAIR_MAX_COMPL_END=9.99 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because one primer has end compl 10.00)" = SEQUENCE_ID=boundary_test_44 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=10.01 PRIMER_PAIR_MAX_COMPL_END=10.01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_45 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=12.01 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=12.01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_46 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=11.99 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_PAIR_MAX_COMPL_ANY=11.99 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because self any for one primer is 12.00" = SEQUENCE_ID=boundary_test_47 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_48 PRIMER_GC_CLAMP=1 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because of GC clamp)" = SEQUENCE_ID=boundary_test_49 PRIMER_GC_CLAMP=1 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_50 PRIMER_GC_CLAMP=2 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because of GC clamp)" = SEQUENCE_ID=boundary_test_51 PRIMER_PAIR_MAX_DIFF_TM=100 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_52 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_53 PRIMER_PAIR_MAX_DIFF_TM=0.28 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (due to max diff tm)" = SEQUENCE_ID=boundary_test_54 PRIMER_LIBERAL_BASE=1 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_55 PRIMER_LIBERAL_BASE=0 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should generate error" = SEQUENCE_ID=poly_x_1 SEQUENCE_TEMPLATE=AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTTCCCCCC PRIMER_MAX_POLY_X=5 PRIMER_INTERNAL_MAX_POLY_X=6 PRIMER_PRODUCT_SIZE_RANGE=50-300 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PAIR_MAX_DIFF_TM=100 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 = SEQUENCE_ID=poly_x_2 PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 SEQUENCE_TEMPLATE=AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG = SEQUENCE_ID=poly_x_3 SEQUENCE_TEMPLATE=AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTTGGGGGGGGGGGGGG PRIMER_MAX_POLY_X=6 PRIMER_INTERNAL_MAX_POLY_X=5 = SEQUENCE_ID=boundary_test_59 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=ok (excluded region is between G and N and end of left primer). = SEQUENCE_ID=boundary_test_60 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (excluded region is between T and G at end of left primer). = SEQUENCE_ID=boundary_test_61 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,-1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (negative excluded region size). = SEQUENCE_ID=boundary_test_62 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=2,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED REGION = SEQUENCE_ID=boundary_test_63 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=3,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers = SEQUENCE_ID=boundary_test_64 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=60,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with EXCLUDED_REGION outside of sequence = SEQUENCE_ID=boundary_test_65 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=59,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED_REGION = SEQUENCE_ID=boundary_test_66 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=57,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers = SEQUENCE_ID=boundary_test_67 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=57,2 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to pick primers and warn EXCLUDED_REGION outside of INCLUDED_REGION = SEQUENCE_ID=boundary_test_68 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,0 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=ok (TARGET is between G and N and end of left primer). = SEQUENCE_ID=boundary_test_69 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (TARGET is between T and G at end of left primer). = SEQUENCE_ID=boundary_test_70 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,-1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (negative TARGET size). = SEQUENCE_ID=boundary_test_71 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=2,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to find legal primers, and warn TARGET outside of INCLUDED REGION = SEQUENCE_ID=boundary_test_72 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=3,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers = SEQUENCE_ID=boundary_test_73 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=60,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with TARGET outside of sequence = SEQUENCE_ID=boundary_test_74 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=59,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to find primers (none spans target), and warn TARGET outside of INCLUDED_REGION = SEQUENCE_ID=boundary_test_75 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=57,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers = SEQUENCE_ID=boundary_test_76 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=57,2 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to pick primers, and warn TARGET outside of INCLUDED_REGION = SEQUENCE_ID=boundary_test_77 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=28,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (target is at TTG^NNN) = SEQUENCE_ID=boundary_test_78 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=28,5 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (target is the middle run of N) = SEQUENCE_ID=boundary_test_79 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=28,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_80 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=32,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_81 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=33,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_82 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=1,59 SEQUENCE_TARGET=33,0 1,1 59,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_83 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=1,59 SEQUENCE_TARGET=1,1 59,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (no target) = SEQUENCE_ID=boundary_test_84 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=1,59 SEQUENCE_TARGET=1,1 33,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_85 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=1,59 SEQUENCE_TARGET=33,0 59,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_86 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=35 PRIMER_MAX_SIZE=35 PRIMER_OPT_SIZE=35 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=75-75 SEQUENCE_TEMPLATE=NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (testing hard limit MAX_PRIMER_LENGTH) = SEQUENCE_ID=boundary_test_71 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=2,1 28,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should warn TARGET outside of INCLUDED REGION = SEQUENCE_ID=boundary_test_72 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,56 PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succed with exactly one pair = SEQUENCE_ID=boundary_test_73 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,56 PRIMER_PRODUCT_SIZE_RANGE=54-54 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (no primers with acceptable product size) = SEQUENCE_ID=boundary_test_74 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,56 PRIMER_PRODUCT_SIZE_RANGE=54-55 55-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair = SEQUENCE_ID=boundary_test_75 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair = SEQUENCE_ID=boundary_test_76 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,56 PRIMER_PRODUCT_SIZE_RANGE=55-55 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair = SEQUENCE_ID=boundary_test_77 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,82 PRIMER_PRODUCT_SIZE_RANGE=55-55 82-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product second = SEQUENCE_ID=boundary_test_78 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,82 PRIMER_PRODUCT_SIZE_RANGE=82-82 55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product first = SEQUENCE_ID=boundary_test_79 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,82 PRIMER_PRODUCT_SIZE_RANGE=55-55 55-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product second = SEQUENCE_ID=boundary_test_80 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,82 PRIMER_PRODUCT_SIZE_RANGE=82-82 55-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product first = SEQUENCE_ID=boundary_test_81 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing pairwise complementarity checking) = SEQUENCE_ID=boundary_test_82 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=10.99 PRIMER_PAIR_MAX_COMPL_ANY=10.99 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (self any for pairs) = SEQUENCE_ID=boundary_test_83 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing pairwise complementarity checking) = SEQUENCE_ID=boundary_test_84 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=9.99 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=9.99 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (self end for pairs) = SEQUENCE_ID=boundary_test_85 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of product size ranges) = SEQUENCE_ID=boundary_test_86 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TARGET=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of targets) = SEQUENCE_ID=boundary_test_87 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TARGET=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (testing number of targets) = SEQUENCE_ID=boundary_test_88 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_EXCLUDED_REGION=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of excluded regions) = SEQUENCE_ID=boundary_test_89 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_EXCLUDED_REGION=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (testing number of excluded regions) = primer3-2.3.6/test/repeat_lib20000664000077300007730000000000012231156245015136 0ustar ioanaioanaprimer3-2.3.6/test/primer_gc_end_input0000664000077300007730000003005012231156245016772 0ustar ioanaioanaSEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=5 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=4 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=3 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=2 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=1 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=0 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 = primer3-2.3.6/test/primer_mispriming_boundary2_input0000664000077300007730000000235212231156245021722 0ustar ioanaioanaSEQUENCE_ID=pair_1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY=primer_repeat_boundary2_lib PRIMER_INTERNAL_MISHYB_LIBRARY=primer_repeat_boundary2_lib SEQUENCE_TEMPLATE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_LIBRARY_MISPRIMING=1 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=1 PRIMER_MAX_SELF_ANY=25 PRIMER_MAX_SELF_END=25 PRIMER_PAIR_MAX_COMPL_ANY=25 PRIMER_PAIR_MAX_COMPL_END=25 PRIMER_INTERNAL_MAX_SELF_ANY=25 PRIMER_INTERNAL_MAX_SELF_END=25 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_SIZE=20 PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_INTERNAL_MAX_GC=100 PRIMER_MAX_SIZE=20 PRIMER_MIN_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MAX_TM=100 PRIMER_MIN_TM=0 PRIMER_OPT_TM=60 = SEQUENCE_ID=pair_0 P3_COMMENT="Should fail because of high repeat similarity" PRIMER_MAX_LIBRARY_MISPRIMING=20 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=20 SEQUENCE_TEMPLATE=TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA = primer3-2.3.6/test/primer_boundary_formatted_output0000664000077300007730000037566212231156245021671 0ustar ioanaioanaPRIMER PICKING RESULTS FOR boundary_test_00 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_01 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_02 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_03 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, unacceptable product size 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_04 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, unacceptable product size 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_05 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 56 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 4, unacceptable product size 3, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_06 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 5 0 0 0 0 0 0 0 0 0 0 0 0 Right 5 5 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_07 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_08 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ... . KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 6 6 0 0 0 0 0 0 0 0 0 0 0 0 Right 6 5 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_09 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 54 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. ... KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 5 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_10 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 0 NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 5 0 0 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_11 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 61.18 33.33 12.00 9.50 CACGTCATCTNATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.75 0 NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 2 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 3, unacceptable product size 2, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_11 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 0 NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 5 0 0 0 0 0 0 0 0 0 0 0 0 Right 5 2 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_12 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_13 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 0 NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_14 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 0 NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 1 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_15 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 90.45 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGGG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00 0 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_16 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 0 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 1 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 1 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_17 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_18 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ... . KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 1 0 0 0 0 0 0 0 0 0 0 0 0 Right 1 1 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_19 INPUT PROBLEM: SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE PRIMER PICKING RESULTS FOR boundary_test_20 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 54 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. ... KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 1 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_21 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 27,5 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_22 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 26,6 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ****** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 2 2 0 0 0 0 0 0 0 0 0 0 0 0 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_23 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 27,6 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ****** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 2 2 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_24 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 28,5 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ***** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 2 2 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_25 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 27,1 28,4 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_26 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 26,1 27,5 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ****** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 1 0 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_27 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 27,1 28,5 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ****** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 1 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_28 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 28,1 29,4 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ***** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 1 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_29 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> * <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_30 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 28,0 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_31 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 0,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN * KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, no target 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_32 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 27,5 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>>XXXXX<<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_33 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 26,6 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_34 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 27,6 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_35 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 28,5 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_36 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 27,1 28,4 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>>XXXXX<<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_37 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 26,1 27,5 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_37 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 27,1 28,5 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_38 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 28,1 29,4 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_39 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_40 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 28,0 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_41 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 10,0 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_42 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_43 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN X KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 1 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_44 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_45 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_46 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN X KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 1 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_47 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_48 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN X KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 1 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_49 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_50 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN X KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 1 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_51 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_52 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_53 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN X KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, tm diff too large 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_54 No mispriming library specified Using 0-based sequence positions WARNING: Unrecognized base in input sequence OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 EXCLUDED REGIONS (start, len)*: 28,1 0 NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_55 INPUT PROBLEM: Unrecognized base in input sequence PRIMER PICKING RESULTS FOR poly_x_1 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 187 INCLUDED REGION SIZE: 187 0 AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGG 60 TTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTT 120 GGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTT 180 TCCCCCC Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 138 0 0 0 0 0 0 0 0 0 0 138 0 0 Right 138 0 0 0 0 0 0 0 0 0 0 138 0 0 Intl 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR poly_x_2 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 29 25 67.77 40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC RIGHT PRIMER 130 25 67.77 40.00 12.00 3.00 GAAAAATTTTTCCCCCAAAAAGGGG INTERNAL OLIGO 87 19 60.50 47.37 5.00 3.00 CCCTTTTTGGGGGAAAAAC SEQUENCE SIZE: 180 INCLUDED REGION SIZE: 180 PRODUCT SIZE: 102, PAIR ANY COMPL: 22.00, PAIR 3' COMPL: 14.00 0 AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGG >>>>>>>>>>>>>>>>>>>>>>>>> 60 AAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG ^^^^^^^^^^^^^^^^^^^<<<<<<<<<<<<<< 120 AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG <<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 29 25 67.77 40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC RIGHT PRIMER 131 25 67.77 40.00 14.00 1.00 GGAAAAATTTTTCCCCCAAAAAGGG INTERNAL OLIGO 88 19 60.50 47.37 5.00 1.00 CCTTTTTGGGGGAAAAACC PRODUCT SIZE: 103, PAIR ANY COMPL: 23.00, PAIR 3' COMPL: 19.00 2 LEFT PRIMER 29 25 67.77 40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC RIGHT PRIMER 132 25 67.77 40.00 16.00 1.00 GGGAAAAATTTTTCCCCCAAAAAGG INTERNAL OLIGO 89 19 60.50 47.37 5.00 1.00 CTTTTTGGGGGAAAAACCC PRODUCT SIZE: 104, PAIR ANY COMPL: 24.00, PAIR 3' COMPL: 22.00 3 LEFT PRIMER 29 25 67.77 40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC RIGHT PRIMER 133 25 67.77 40.00 18.00 3.00 GGGGAAAAATTTTTCCCCCAAAAAG INTERNAL OLIGO 89 19 60.50 47.37 5.00 1.00 CTTTTTGGGGGAAAAACCC PRODUCT SIZE: 105, PAIR ANY COMPL: 25.00, PAIR 3' COMPL: 25.00 4 LEFT PRIMER 28 25 67.77 40.00 16.00 16.00 CCTTTTTGGGGGAAAAATTTTTCCC RIGHT PRIMER 130 25 67.77 40.00 12.00 3.00 GAAAAATTTTTCCCCCAAAAAGGGG INTERNAL OLIGO 87 19 60.50 47.37 5.00 3.00 CCCTTTTTGGGGGAAAAAC PRODUCT SIZE: 103, PAIR ANY COMPL: 23.00, PAIR 3' COMPL: 19.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 131 0 0 0 0 0 0 0 0 0 0 0 0 131 Right 131 0 0 0 0 0 0 0 0 0 0 0 0 131 Intl 1585 0 0 0 0 0 0 0 0 0 0 0 0 1585 Pair Stats: considered 64, unacceptable product size 48, ok 16 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR poly_x_3 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 204 INCLUDED REGION SIZE: 204 0 AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCC 60 TTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGG 120 GGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCC 180 CCCTTTTTTTGGGGGGGGGGGGGG Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 155 0 0 0 0 0 0 0 0 0 0 57 0 98 Right 155 0 0 0 0 0 0 0 0 0 0 72 0 83 Intl 187 0 0 0 0 0 0 0 0 0 0 187 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_59 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 27,0 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_60 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 26,0 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_61 INPUT PROBLEM: Negative EXCLUDED_REGION length PRIMER PICKING RESULTS FOR boundary_test_62 No mispriming library specified Using 0-based sequence positions WARNING: EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 1,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_63 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 EXCLUDED REGIONS (start, len)*: 2,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..X .. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 1 0 0 0 0 0 0 0 0 0 0 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_64 INPUT PROBLEM: EXCLUDED_REGION beyond end of sequence PRIMER PICKING RESULTS FOR boundary_test_65 No mispriming library specified Using 0-based sequence positions WARNING: EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 58,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_66 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 EXCLUDED REGIONS (start, len)*: 56,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. X.. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_67 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND WARNING: EXCLUDED_REGION outside of INCLUDED_REGION SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 EXCLUDED REGIONS (start, len)*: 56,2 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. X.. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 6 5 0 0 0 0 0 0 0 0 0 0 0 1 Right 6 5 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_68 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 27,0 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_69 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 26,0 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 2 2 0 0 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_70 INPUT PROBLEM: Negative TARGET length PRIMER PICKING RESULTS FOR boundary_test_71 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND WARNING: TARGET outside of INCLUDED_REGION SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 TARGETS (start, len)*: 1,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. .. KEYS (in order of precedence): ...... vector sequence ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, no target 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_72 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 TARGETS (start, len)*: 2,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..* .. KEYS (in order of precedence): ...... vector sequence ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 1 0 0 0 0 0 0 0 0 0 0 0 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_73 INPUT PROBLEM: TARGET beyond end of sequence PRIMER PICKING RESULTS FOR boundary_test_74 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND WARNING: TARGET outside of INCLUDED_REGION SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 TARGETS (start, len)*: 58,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. .. KEYS (in order of precedence): ...... vector sequence ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, no target 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_75 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 TARGETS (start, len)*: 56,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. *.. KEYS (in order of precedence): ...... vector sequence ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_76 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND WARNING: TARGET outside of INCLUDED_REGION SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 TARGETS (start, len)*: 56,2 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. *.. KEYS (in order of precedence): ...... vector sequence ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 6 5 0 0 0 0 0 0 0 0 0 0 0 1 Right 6 5 1 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_77 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 27,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_78 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 27,5 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_79_1 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 27,0 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 6 5 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_80 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 31,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> *<<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_81 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 32,0 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 6 5 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_82 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 32,0 0,1 58,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN * >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< * KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_83 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 0,1 58,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN * * KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, no target 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_84 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 0,1 32,0 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN * >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 8 7 0 0 0 0 0 0 0 0 0 0 0 1 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_85 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 32,0 58,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< * KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_86 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 35 62.84 25.71 12.00 10.00 CACGTCATCTATATATATATCATCAAATTTGATTG RIGHT PRIMER 76 35 61.36 25.71 10.00 8.00 GAATTGTTTGGAAATGACAGTATATATATATGAGT SEQUENCE SIZE: 79 INCLUDED REGION SIZE: 79 PRODUCT SIZE: 75, PAIR ANY COMPL: 13.00, PAIR 3' COMPL: 8.00 0 NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTG >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<< 60 TCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_71_2 No mispriming library specified Using 0-based sequence positions WARNING: TARGET outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 1,1 27,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_72_2 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 56 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< . KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 3, unacceptable product size 2, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_73_2 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 56 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. . KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 2, unacceptable product size 2, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_74_2 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 56 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< . KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_75_2 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 2 1 0 0 0 0 0 0 0 0 0 0 0 1 Right 2 1 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 2, unacceptable product size 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_76_2 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 56 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< . KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 2 1 0 0 0 0 0 0 0 0 0 0 0 1 Right 2 1 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 2, unacceptable product size 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_77_2 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 86 INCLUDED REGION SIZE: 82 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< 60 CTCACTGTCATTTCCAAACAATTCNN .. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 83 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 28 27 0 0 0 0 0 0 0 0 0 0 0 1 Right 28 26 0 0 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 4, unacceptable product size 2, ok 2 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_78_2 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 83 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 86 INCLUDED REGION SIZE: 82 PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA ..>>>>>>>>>>>>>>>>>>>>>>>>> < 60 CTCACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 28 27 0 0 0 0 0 0 0 0 0 0 0 1 Right 28 26 0 0 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 4, unacceptable product size 2, ok 2 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_79_2 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 86 INCLUDED REGION SIZE: 82 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< 60 CTCACTGTCATTTCCAAACAATTCNN .. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 83 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 28 27 0 0 0 0 0 0 0 0 0 0 0 1 Right 28 26 0 0 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 3, unacceptable product size 1, ok 2 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_80_2 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 83 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 86 INCLUDED REGION SIZE: 82 PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA ..>>>>>>>>>>>>>>>>>>>>>>>>> < 60 CTCACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 28 27 0 0 0 0 0 0 0 0 0 0 0 1 Right 28 26 0 0 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 3, unacceptable product size 1, ok 2 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_81_2 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG RIGHT PRIMER 56 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_82_2 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN .. .. KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, high any compl 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_83_2 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG RIGHT PRIMER 56 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_84_2 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN .. .. KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, high end compl 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_85_2 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG RIGHT PRIMER 56 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_86 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG RIGHT PRIMER 56 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00 TARGETS (start, len)*: 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_87 INPUT PROBLEM: Too many elements for tag SEQUENCE_TARGET PRIMER PICKING RESULTS FOR boundary_test_88 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG RIGHT PRIMER 56 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00 EXCLUDED REGIONS (start, len)*: 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 0 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>>X <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_89 INPUT PROBLEM: Too many elements for tag SEQUENCE_EXCLUDED_REGION PRIMER PICKING RESULTS FOR boundary_test_90 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_91 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 0 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 1 0 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_92 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG POSITION PENALTY, QUALITY: 0.000000, 2.731673 RIGHT PRIMER 56 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT POSITION PENALTY, QUALITY: 400.000000, 400.649600 SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 27,1 0 ATCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCGG >>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 2 0 0 0 0 0 0 0 0 0 0 0 3 Right 5 2 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 9, unacceptable product size 8, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_93 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 25 60.94 32.00 12.00 12.00 ATCACGTCATCTCATCAAATTTGAT POSITION PENALTY, QUALITY: 200.000000, 200.942558 RIGHT PRIMER 54 25 58.09 32.00 4.00 2.00 ATTGTTTGGAAATGACAGTGAGTAT POSITION PENALTY, QUALITY: 200.000000, 201.906454 SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 27,1 0 ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG >>>>>>>>>>>>>>>>>>>>>>>>> * <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_94 No mispriming library specified Using 0-based sequence positions WARNING: Non-default inside penalty or outside penalty has no effect when number of targets is 0 OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 25 60.94 32.00 12.00 12.00 ATCACGTCATCTCATCAAATTTGAT POSITION PENALTY, QUALITY: 0.000000, 0.942558 RIGHT PRIMER 54 25 58.09 32.00 4.00 2.00 ATTGTTTGGAAATGACAGTGAGTAT POSITION PENALTY, QUALITY: 0.000000, 1.906454 SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 2.00 0 ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_95 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 25 60.94 32.00 12.00 12.00 ATCACGTCATCTCATCAAATTTGAT POSITION PENALTY, QUALITY: 0.000000, 0.942558 RIGHT PRIMER 54 25 58.56 36.00 4.00 4.00 ATTGTTTGGAAATGACAGTGAGTAC POSITION PENALTY, QUALITY: 7000.000000, 7001.440912 SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 27,10 0 ATCACGTCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATNCGG >>>>>>>>>>>>>>>>>>>>>>>>> ***(((((((<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 5, unacceptable product size 4, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_96 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT POSITION PENALTY, QUALITY: 2000.000000, 2000.754679 RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA POSITION PENALTY, QUALITY: 3000.000000, 3004.155585 SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 27,10 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG >>>>>>>>>>>>>>>>>>>>>>>))*****(((<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_97 No mispriming library specified Using 0-based sequence positions WARNING: Non-default inside penalty or outside penalty has no effect when number of targets is 0 OLIGO start len tm gc% any 3' seq LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT POSITION PENALTY, QUALITY: 0.000000, 0.754679 RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA POSITION PENALTY, QUALITY: 0.000000, 4.155585 SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR test_098 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 PRODUCT Tm: 62.4120, PRODUCT Tm - min(OLIGO Tm): 3.1667 0 ATCNCATCATCTCATCAAATTTGATTGATTTTACTCACTGTCATTTCCAAACAATACGG >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR test_099 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, high product Tm 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR test_100 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR test_101 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 PRODUCT Tm: 63.9029, PRODUCT Tm - min(OLIGO Tm): 4.6576 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR test_102 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, low product Tm 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR test_103 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 4 25 59.25 28.00 12.00 12.00 CATCATCTCATCAAATTTGATTGAT RIGHT PRIMER 58 25 64.16 40.00 4.00 3.00 CCGTATTGTTTGGAAATGACAGTGA SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 0 ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, ok 1 libprimer3 release 2.2.1 primer3-2.3.6/test/primer_internal1_output0000664000077300007730000014431212231156245017660 0ustar ioanaioanaPRIMER_FIRST_BASE_INDEX=1 SEQUENCE_ID=boundary_test_00 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_INTERNAL_MIN_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_INTERNAL_MAX_SELF_ANY=100 PRIMER_INTERNAL_MAX_SELF_END=100 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_INTERNAL_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, no internal oligo 2, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-100 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 30, too many Ns 28, ok 2 PRIMER_RIGHT_EXPLAIN=considered 30, too many Ns 29, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=2.731673 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=62.732 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=10.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_02 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=2.731673 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=62.732 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=10.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_05 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_MIN_SIZE=24 PRIMER_MAX_SIZE=24 PRIMER_OPT_SIZE=24 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.330996 PRIMER_LEFT_0_PENALTY=0.243965 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=2.731673 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_LEFT_0=3,24 PRIMER_RIGHT_0=82,24 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=60.244 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=62.732 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=10.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 1, ok 4 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 59, ok 1 PRIMER_PAIR_EXPLAIN=considered 8, unacceptable product size 6, no internal oligo 2, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=2 PRIMER_INTERNAL_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 52, ok 8 PRIMER_PAIR_EXPLAIN=considered 10, unacceptable product size 8, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=2 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=0.893850 PRIMER_LEFT_0_PENALTY=0.806819 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=3.221174 PRIMER_LEFT_0_SEQUENCE=CACGTCATCNNATCAAATTTGATT PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCNNATCAAATTTGATTG PRIMER_LEFT_0=3,24 PRIMER_RIGHT_0=82,24 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=60.807 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=63.221 PRIMER_LEFT_0_GC_PERCENT=31.818 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=11.50 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=11.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=2.546959 PRIMER_LEFT_1_PENALTY=0.806819 PRIMER_RIGHT_1_PENALTY=1.740140 PRIMER_INTERNAL_1_PENALTY=3.221174 PRIMER_LEFT_1_SEQUENCE=ACGTCATCNNATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=NGAATTGTTTGGAAATGACAGTGA PRIMER_INTERNAL_1_SEQUENCE=CACGTCATCNNATCAAATTTGATTG PRIMER_LEFT_1=4,24 PRIMER_RIGHT_1=83,24 PRIMER_INTERNAL_1=30,25 PRIMER_LEFT_1_TM=60.807 PRIMER_RIGHT_1_TM=61.740 PRIMER_INTERNAL_1_TM=63.221 PRIMER_LEFT_1_GC_PERCENT=31.818 PRIMER_RIGHT_1_GC_PERCENT=34.783 PRIMER_INTERNAL_1_GC_PERCENT=34.783 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=12.00 PRIMER_LEFT_1_SELF_END=11.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=11.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_12 PRIMER_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 52, ok 8 PRIMER_PAIR_EXPLAIN=considered 10, unacceptable product size 8, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=2 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=0.893850 PRIMER_LEFT_0_PENALTY=0.806819 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=3.221174 PRIMER_LEFT_0_SEQUENCE=CACGTCATCNNATCAAATTTGATT PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=CACGTCATCNNATCAAATTTGATTG PRIMER_LEFT_0=3,24 PRIMER_RIGHT_0=82,24 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=60.807 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=63.221 PRIMER_LEFT_0_GC_PERCENT=31.818 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=11.50 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=11.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=2.546959 PRIMER_LEFT_1_PENALTY=0.806819 PRIMER_RIGHT_1_PENALTY=1.740140 PRIMER_INTERNAL_1_PENALTY=3.221174 PRIMER_LEFT_1_SEQUENCE=ACGTCATCNNATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=NGAATTGTTTGGAAATGACAGTGA PRIMER_INTERNAL_1_SEQUENCE=CACGTCATCNNATCAAATTTGATTG PRIMER_LEFT_1=4,24 PRIMER_RIGHT_1=83,24 PRIMER_INTERNAL_1=30,25 PRIMER_LEFT_1_TM=60.807 PRIMER_RIGHT_1_TM=61.740 PRIMER_INTERNAL_1_TM=63.221 PRIMER_LEFT_1_GC_PERCENT=31.818 PRIMER_RIGHT_1_GC_PERCENT=34.783 PRIMER_INTERNAL_1_GC_PERCENT=34.783 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=12.00 PRIMER_LEFT_1_SELF_END=11.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=11.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_13 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 23, ok 37 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=5.394230 PRIMER_LEFT_0_PENALTY=5.307199 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=3.800256 PRIMER_LEFT_0_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAA PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTNN PRIMER_LEFT_0=3,24 PRIMER_RIGHT_0=82,24 PRIMER_INTERNAL_0=32,25 PRIMER_LEFT_0_TM=54.693 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=56.200 PRIMER_LEFT_0_GC_PERCENT=0.000 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=0.000 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_14 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=.0001 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 2, ok 3 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 25, ok 35 PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.843553 PRIMER_LEFT_0_PENALTY=4.756523 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=3.800256 PRIMER_LEFT_0_SEQUENCE=AAAAAAAAAAAAAAAAACAAAAAA PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTNN PRIMER_LEFT_0=3,24 PRIMER_RIGHT_0=82,24 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=55.243 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=56.200 PRIMER_LEFT_0_GC_PERCENT=4.167 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=0.000 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_15 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=20 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 25, ok 35 PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=29.395694 PRIMER_LEFT_0_PENALTY=29.308664 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=4.161950 PRIMER_LEFT_0_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=TTTTTTTTTTTTTTTTTTTTTTTTT PRIMER_LEFT_0=3,24 PRIMER_RIGHT_0=82,24 PRIMER_INTERNAL_0=33,25 PRIMER_LEFT_0_TM=89.309 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=55.838 PRIMER_LEFT_0_GC_PERCENT=100.000 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=0.000 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=1.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_16 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_PRODUCT_SIZE_RANGE=50-200 PRIMER_INTERNAL_MAX_NS_ACCEPTED=5 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 8, ok 2 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 8, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 35, ok 35 PRIMER_PAIR_EXPLAIN=considered 4, no internal oligo 4, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_17 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=20 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 31, ok 4 PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 31, ok 4 PRIMER_INTERNAL_EXPLAIN=considered 60, GC content failed 29, ok 31 PRIMER_PAIR_EXPLAIN=considered 16, unacceptable product size 4, no internal oligo 12, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_18 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INTERNAL_EXCLUDED_REGION=11,50 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 62, too many Ns 53, ok 9 PRIMER_RIGHT_EXPLAIN=considered 62, too many Ns 53, ok 9 PRIMER_INTERNAL_EXPLAIN=considered 87, too many Ns 39, overlap excluded region 40, ok 8 PRIMER_PAIR_EXPLAIN=considered 81, unacceptable product size 49, no internal oligo 32, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_19 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 SEQUENCE_INTERNAL_EXCLUDED_REGION=11,50 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNNCCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 66, too many Ns 57, ok 9 PRIMER_RIGHT_EXPLAIN=considered 66, too many Ns 57, ok 9 PRIMER_INTERNAL_EXPLAIN=considered 91, too many Ns 84, overlap excluded region 5, ok 2 PRIMER_PAIR_EXPLAIN=considered 16, unacceptable product size 3, no internal oligo 8, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.330996 PRIMER_LEFT_0_PENALTY=0.243965 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=5.578209 PRIMER_LEFT_0_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_0=4,24 PRIMER_RIGHT_0=113,24 PRIMER_INTERNAL_0=61,25 PRIMER_LEFT_0_TM=60.244 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=65.578 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=52.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=110 PRIMER_PAIR_1_PENALTY=0.330996 PRIMER_LEFT_1_PENALTY=0.243965 PRIMER_RIGHT_1_PENALTY=0.087031 PRIMER_INTERNAL_1_PENALTY=5.578209 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_1_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_1=3,24 PRIMER_RIGHT_1=113,24 PRIMER_INTERNAL_1=61,25 PRIMER_LEFT_1_TM=60.244 PRIMER_RIGHT_1_TM=59.913 PRIMER_INTERNAL_1_TM=65.578 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=37.500 PRIMER_INTERNAL_1_GC_PERCENT=52.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=111 PRIMER_PAIR_2_PENALTY=1.261475 PRIMER_LEFT_2_PENALTY=0.243965 PRIMER_RIGHT_2_PENALTY=1.017510 PRIMER_INTERNAL_2_PENALTY=5.578209 PRIMER_LEFT_2_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_2_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_2_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_2=4,24 PRIMER_RIGHT_2=112,24 PRIMER_INTERNAL_2=61,25 PRIMER_LEFT_2_TM=60.244 PRIMER_RIGHT_2_TM=58.982 PRIMER_INTERNAL_2_TM=65.578 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=33.333 PRIMER_INTERNAL_2_GC_PERCENT=52.000 PRIMER_LEFT_2_SELF_ANY=12.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=10.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=109 PRIMER_PAIR_3_PENALTY=1.261475 PRIMER_LEFT_3_PENALTY=0.243965 PRIMER_RIGHT_3_PENALTY=1.017510 PRIMER_INTERNAL_3_PENALTY=5.578209 PRIMER_LEFT_3_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_3_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_3=3,24 PRIMER_RIGHT_3=112,24 PRIMER_INTERNAL_3=61,25 PRIMER_LEFT_3_TM=60.244 PRIMER_RIGHT_3_TM=58.982 PRIMER_INTERNAL_3_TM=65.578 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=52.000 PRIMER_LEFT_3_SELF_ANY=12.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=10.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=110 PRIMER_PAIR_4_PENALTY=8.005859 PRIMER_LEFT_4_PENALTY=7.918829 PRIMER_RIGHT_4_PENALTY=0.087031 PRIMER_INTERNAL_4_PENALTY=5.578209 PRIMER_LEFT_4_SEQUENCE=AAGCTAAGCTTGCCCGTATCCCCT PRIMER_RIGHT_4_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_4_SEQUENCE=CCGTTAGGTGCTAGCCGTGTAGATT PRIMER_LEFT_4=30,24 PRIMER_RIGHT_4=113,24 PRIMER_INTERNAL_4=61,25 PRIMER_LEFT_4_TM=67.919 PRIMER_RIGHT_4_TM=59.913 PRIMER_INTERNAL_4_TM=65.578 PRIMER_LEFT_4_GC_PERCENT=54.167 PRIMER_RIGHT_4_GC_PERCENT=37.500 PRIMER_INTERNAL_4_GC_PERCENT=52.000 PRIMER_LEFT_4_SELF_ANY=9.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=10.9000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=84 = SEQUENCE_ID=boundary_test_20 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=100-300 SEQUENCE_INTERNAL_EXCLUDED_REGION=6,50 SEQUENCE_INTERNAL_EXCLUDED_REGION=61,15 SEQUENCE_INCLUDED_REGION=3,250 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATACGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT PRIMER_LEFT_EXPLAIN=considered 151, too many Ns 99, ok 52 PRIMER_RIGHT_EXPLAIN=considered 151, too many Ns 148, ok 3 PRIMER_INTERNAL_EXPLAIN=considered 226, too many Ns 175, overlap excluded region 47, ok 4 PRIMER_PAIR_EXPLAIN=considered 14, no internal oligo 8, ok 6 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.330996 PRIMER_LEFT_0_PENALTY=0.243965 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=3.811648 PRIMER_LEFT_0_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG PRIMER_LEFT_0=4,24 PRIMER_RIGHT_0=252,24 PRIMER_INTERNAL_0=76,25 PRIMER_LEFT_0_TM=60.244 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=63.812 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=52.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=12.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=249 PRIMER_PAIR_1_PENALTY=0.330996 PRIMER_LEFT_1_PENALTY=0.243965 PRIMER_RIGHT_1_PENALTY=0.087031 PRIMER_INTERNAL_1_PENALTY=3.811648 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_1_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG PRIMER_LEFT_1=3,24 PRIMER_RIGHT_1=252,24 PRIMER_INTERNAL_1=76,25 PRIMER_LEFT_1_TM=60.244 PRIMER_RIGHT_1_TM=59.913 PRIMER_INTERNAL_1_TM=63.812 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=37.500 PRIMER_INTERNAL_1_GC_PERCENT=52.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=12.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=12.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=250 PRIMER_PAIR_2_PENALTY=0.386435 PRIMER_LEFT_2_PENALTY=0.299404 PRIMER_RIGHT_2_PENALTY=0.087031 PRIMER_INTERNAL_2_PENALTY=3.811648 PRIMER_LEFT_2_SEQUENCE=GGAGACGATACGGTAAGCTAGCTA PRIMER_RIGHT_2_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_2_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG PRIMER_LEFT_2=51,24 PRIMER_RIGHT_2=252,24 PRIMER_INTERNAL_2=76,25 PRIMER_LEFT_2_TM=60.299 PRIMER_RIGHT_2_TM=59.913 PRIMER_INTERNAL_2_TM=63.812 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=37.500 PRIMER_INTERNAL_2_GC_PERCENT=52.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=12.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=12.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=202 PRIMER_PAIR_3_PENALTY=1.261475 PRIMER_LEFT_3_PENALTY=0.243965 PRIMER_RIGHT_3_PENALTY=1.017510 PRIMER_INTERNAL_3_PENALTY=3.811648 PRIMER_LEFT_3_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_3_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG PRIMER_LEFT_3=4,24 PRIMER_RIGHT_3=251,24 PRIMER_INTERNAL_3=76,25 PRIMER_LEFT_3_TM=60.244 PRIMER_RIGHT_3_TM=58.982 PRIMER_INTERNAL_3_TM=63.812 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=52.000 PRIMER_LEFT_3_SELF_ANY=12.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=12.00 PRIMER_LEFT_3_SELF_END=10.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=12.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=248 PRIMER_PAIR_4_PENALTY=1.261475 PRIMER_LEFT_4_PENALTY=0.243965 PRIMER_RIGHT_4_PENALTY=1.017510 PRIMER_INTERNAL_4_PENALTY=3.811648 PRIMER_LEFT_4_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_4_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_4_SEQUENCE=CTGGTCAAAGCTAGCGGTAGCTTAG PRIMER_LEFT_4=3,24 PRIMER_RIGHT_4=251,24 PRIMER_INTERNAL_4=76,25 PRIMER_LEFT_4_TM=60.244 PRIMER_RIGHT_4_TM=58.982 PRIMER_INTERNAL_4_TM=63.812 PRIMER_LEFT_4_GC_PERCENT=33.333 PRIMER_RIGHT_4_GC_PERCENT=33.333 PRIMER_INTERNAL_4_GC_PERCENT=52.000 PRIMER_LEFT_4_SELF_ANY=12.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=12.00 PRIMER_LEFT_4_SELF_END=10.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=12.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=249 = SEQUENCE_ID=boundary_test_21 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_INTERNAL_MIN_TM=55.7 PRIMER_INTERNAL_MAX_TM=55.9 PRIMER_INTERNAL_OPT_TM=55.8 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 77, too many Ns 27, ok 50 PRIMER_RIGHT_EXPLAIN=considered 77, too many Ns 28, ok 49 PRIMER_INTERNAL_EXPLAIN=considered 152, too many Ns 57, low tm 1, high tm 93, ok 1 PRIMER_PAIR_EXPLAIN=considered 323, unacceptable product size 223, no internal oligo 87, ok 13 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.330996 PRIMER_LEFT_0_PENALTY=0.243965 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_INTERNAL_0_PENALTY=0.038050 PRIMER_LEFT_0_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_LEFT_0=4,24 PRIMER_RIGHT_0=174,24 PRIMER_INTERNAL_0=62,25 PRIMER_LEFT_0_TM=60.244 PRIMER_RIGHT_0_TM=59.913 PRIMER_INTERNAL_0_TM=55.838 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=0.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=171 PRIMER_PAIR_1_PENALTY=0.330996 PRIMER_LEFT_1_PENALTY=0.243965 PRIMER_RIGHT_1_PENALTY=0.087031 PRIMER_INTERNAL_1_PENALTY=0.038050 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_1_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_LEFT_1=3,24 PRIMER_RIGHT_1=174,24 PRIMER_INTERNAL_1=62,25 PRIMER_LEFT_1_TM=60.244 PRIMER_RIGHT_1_TM=59.913 PRIMER_INTERNAL_1_TM=55.838 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=37.500 PRIMER_INTERNAL_1_GC_PERCENT=0.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=0.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=172 PRIMER_PAIR_2_PENALTY=1.261475 PRIMER_LEFT_2_PENALTY=0.243965 PRIMER_RIGHT_2_PENALTY=1.017510 PRIMER_INTERNAL_2_PENALTY=0.038050 PRIMER_LEFT_2_SEQUENCE=ACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_2_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_2_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_LEFT_2=4,24 PRIMER_RIGHT_2=173,24 PRIMER_INTERNAL_2=62,25 PRIMER_LEFT_2_TM=60.244 PRIMER_RIGHT_2_TM=58.982 PRIMER_INTERNAL_2_TM=55.838 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=33.333 PRIMER_INTERNAL_2_GC_PERCENT=0.000 PRIMER_LEFT_2_SELF_ANY=12.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=0.00 PRIMER_LEFT_2_SELF_END=10.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=170 PRIMER_PAIR_3_PENALTY=1.261475 PRIMER_LEFT_3_PENALTY=0.243965 PRIMER_RIGHT_3_PENALTY=1.017510 PRIMER_INTERNAL_3_PENALTY=0.038050 PRIMER_LEFT_3_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_3_SEQUENCE=AATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_3_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_LEFT_3=3,24 PRIMER_RIGHT_3=173,24 PRIMER_INTERNAL_3=62,25 PRIMER_LEFT_3_TM=60.244 PRIMER_RIGHT_3_TM=58.982 PRIMER_INTERNAL_3_TM=55.838 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=0.000 PRIMER_LEFT_3_SELF_ANY=12.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=0.00 PRIMER_LEFT_3_SELF_END=10.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=171 PRIMER_PAIR_4_PENALTY=29.395694 PRIMER_LEFT_4_PENALTY=29.308664 PRIMER_RIGHT_4_PENALTY=0.087031 PRIMER_INTERNAL_4_PENALTY=0.038050 PRIMER_LEFT_4_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGG PRIMER_RIGHT_4_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_INTERNAL_4_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_LEFT_4=38,24 PRIMER_RIGHT_4=174,24 PRIMER_INTERNAL_4=62,25 PRIMER_LEFT_4_TM=89.309 PRIMER_RIGHT_4_TM=59.913 PRIMER_INTERNAL_4_TM=55.838 PRIMER_LEFT_4_GC_PERCENT=100.000 PRIMER_RIGHT_4_GC_PERCENT=37.500 PRIMER_INTERNAL_4_GC_PERCENT=0.000 PRIMER_LEFT_4_SELF_ANY=0.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=0.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=12.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=1.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=137 = SEQUENCE_ID=boundary_test_22 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_INTERNAL_MIN_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_INTERNAL_MAX_SELF_ANY=100 PRIMER_INTERNAL_MAX_SELF_END=100 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_INTERNAL_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_PICK_INTERNAL_OLIGO=1 P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_23 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=2,1 P3_COMMENT=should succeed with warning (internal oligo excluded region outside of included region) PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_24 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=3,1 P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_25 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=1,3 P3_COMMENT=should succeed with warning (internal oligo excluded region outside of included region) PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_26 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=30,1 P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_27 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=31,1 P3_COMMENT=should fail w/ no legal internal oligo PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_28 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=55,1 P3_COMMENT=should fail w/ no legal internal oligo PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_29 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=56,1 P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_30 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=82,1 P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_31 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=83,1 P3_COMMENT=should generate warning (external oligo excluded region outside of included region) PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_32 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=82,2 P3_COMMENT=should generate warning (external oligo excluded region outside of included region) PRIMER_WARNING=PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_33 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 P3_COMMENT=should succeed (testing number of internal oligo excluded regions) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 56, too many Ns 53, overlap excluded region 1, ok 2 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=15.695378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=82,25 PRIMER_INTERNAL_0=31,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=boundary_test_34 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=3,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 P3_COMMENT=should fail (testing number of internal oligo excluded regions) PRIMER_ERROR=Too many elements for tag SEQUENCE_INTERNAL_EXCLUDED_REGION = primer3-2.3.6/test/long_seq_output0000664000077300007730000036666312231156245016233 0ustar ioanaioanaSEQUENCE_EXCLUDED_REGION=0, 37 SEQUENCE_EXCLUDED_REGION=447, 285 SEQUENCE_EXCLUDED_REGION=961, 290 SEQUENCE_EXCLUDED_REGION=2016, 262 SEQUENCE_EXCLUDED_REGION=2564, 287 SEQUENCE_EXCLUDED_REGION=2880, 99 SEQUENCE_EXCLUDED_REGION=3489, 263 SEQUENCE_EXCLUDED_REGION=3820, 283 SEQUENCE_EXCLUDED_REGION=4495, 277 SEQUENCE_EXCLUDED_REGION=4807, 286 SEQUENCE_EXCLUDED_REGION=5100, 276 SEQUENCE_EXCLUDED_REGION=6209, 288 SEQUENCE_EXCLUDED_REGION=6828, 89 SEQUENCE_EXCLUDED_REGION=6981, 129 SEQUENCE_EXCLUDED_REGION=7127, 205 SEQUENCE_EXCLUDED_REGION=7388, 132 SEQUENCE_EXCLUDED_REGION=8552, 166 SEQUENCE_EXCLUDED_REGION=8799, 277 SEQUENCE_EXCLUDED_REGION=9101, 196 SEQUENCE_EXCLUDED_REGION=9300, 301 SEQUENCE_EXCLUDED_REGION=10228, 283 SEQUENCE_EXCLUDED_REGION=10530, 281 SEQUENCE_EXCLUDED_REGION=11013, 243 SEQUENCE_EXCLUDED_REGION=11304, 281 SEQUENCE_EXCLUDED_REGION=11626, 255 SEQUENCE_EXCLUDED_REGION=11938, 291 SEQUENCE_EXCLUDED_REGION=12254, 284 SEQUENCE_EXCLUDED_REGION=12557, 147 SEQUENCE_EXCLUDED_REGION=13103, 160 SEQUENCE_EXCLUDED_REGION=13266, 288 SEQUENCE_EXCLUDED_REGION=14765, 732 SEQUENCE_EXCLUDED_REGION=15794, 127 SEQUENCE_EXCLUDED_REGION=15997, 85 SEQUENCE_EXCLUDED_REGION=16402, 51 SEQUENCE_EXCLUDED_REGION=16880, 59 SEQUENCE_EXCLUDED_REGION=17065, 124 SEQUENCE_EXCLUDED_REGION=17708, 65 SEQUENCE_EXCLUDED_REGION=17929, 78 SEQUENCE_EXCLUDED_REGION=18125, 124 SEQUENCE_EXCLUDED_REGION=18356, 65 SEQUENCE_EXCLUDED_REGION=18581, 87 SEQUENCE_EXCLUDED_REGION=18692, 183 SEQUENCE_EXCLUDED_REGION=19248, 275 SEQUENCE_EXCLUDED_REGION=20438, 24 SEQUENCE_EXCLUDED_REGION=20722, 99 SEQUENCE_EXCLUDED_REGION=20870, 151 SEQUENCE_EXCLUDED_REGION=21401, 114 SEQUENCE_EXCLUDED_REGION=21928, 284 SEQUENCE_EXCLUDED_REGION=22834, 155 SEQUENCE_EXCLUDED_REGION=22992, 287 SEQUENCE_EXCLUDED_REGION=23282, 138 SEQUENCE_EXCLUDED_REGION=23435, 69 SEQUENCE_EXCLUDED_REGION=23459, 280 SEQUENCE_EXCLUDED_REGION=23753, 288 SEQUENCE_EXCLUDED_REGION=24338, 290 SEQUENCE_EXCLUDED_REGION=24591, 511 SEQUENCE_EXCLUDED_REGION=26124, 287 SEQUENCE_EXCLUDED_REGION=27286, 276 SEQUENCE_EXCLUDED_REGION=27667, 278 SEQUENCE_EXCLUDED_REGION=28313, 91 SEQUENCE_EXCLUDED_REGION=28585, 286 SEQUENCE_EXCLUDED_REGION=28957, 286 SEQUENCE_EXCLUDED_REGION=29255, 287 SEQUENCE_EXCLUDED_REGION=29573, 45 SEQUENCE_EXCLUDED_REGION=29801, 468 SEQUENCE_EXCLUDED_REGION=30461, 180 SEQUENCE_EXCLUDED_REGION=30701, 173 SEQUENCE_EXCLUDED_REGION=31589, 220 SEQUENCE_EXCLUDED_REGION=31992, 287 SEQUENCE_EXCLUDED_REGION=32545, 277 SEQUENCE_EXCLUDED_REGION=32848, 68 SEQUENCE_EXCLUDED_REGION=32956, 89 SEQUENCE_EXCLUDED_REGION=33079, 279 SEQUENCE_EXCLUDED_REGION=33371, 117 SEQUENCE_EXCLUDED_REGION=33758, 284 SEQUENCE_EXCLUDED_REGION=34333, 282 SEQUENCE_EXCLUDED_REGION=35341, 108 SEQUENCE_EXCLUDED_REGION=36347, 247 SEQUENCE_EXCLUDED_REGION=36687, 275 SEQUENCE_EXCLUDED_REGION=37003, 286 SEQUENCE_EXCLUDED_REGION=37918, 217 SEQUENCE_EXCLUDED_REGION=38979, 286 SEQUENCE_EXCLUDED_REGION=39515, 700 SEQUENCE_EXCLUDED_REGION=40312, 93 SEQUENCE_EXCLUDED_REGION=40510, 77 SEQUENCE_EXCLUDED_REGION=40691, 236 SEQUENCE_EXCLUDED_REGION=41066, 187 SEQUENCE_EXCLUDED_REGION=41700, 94 SEQUENCE_EXCLUDED_REGION=42159, 126 SEQUENCE_EXCLUDED_REGION=42462, 159 SEQUENCE_EXCLUDED_REGION=43291, 218 SEQUENCE_EXCLUDED_REGION=44058, 109 SEQUENCE_EXCLUDED_REGION=44262, 38 SEQUENCE_EXCLUDED_REGION=44682, 275 SEQUENCE_EXCLUDED_REGION=45145, 285 SEQUENCE_EXCLUDED_REGION=45464, 277 SEQUENCE_EXCLUDED_REGION=46126, 276 SEQUENCE_EXCLUDED_REGION=46471, 288 SEQUENCE_EXCLUDED_REGION=46767, 118 SEQUENCE_EXCLUDED_REGION=46888, 282 SEQUENCE_EXCLUDED_REGION=47260, 94 SEQUENCE_EXCLUDED_REGION=47372, 94 SEQUENCE_EXCLUDED_REGION=47495, 134 SEQUENCE_EXCLUDED_REGION=47854, 285 SEQUENCE_EXCLUDED_REGION=48185, 122 SEQUENCE_EXCLUDED_REGION=48310, 284 SEQUENCE_EXCLUDED_REGION=48598, 142 SEQUENCE_EXCLUDED_REGION=49033, 286 SEQUENCE_EXCLUDED_REGION=49926, 273 SEQUENCE_EXCLUDED_REGION=50195, 72 SEQUENCE_EXCLUDED_REGION=50218, 286 SEQUENCE_EXCLUDED_REGION=50994, 262 SEQUENCE_EXCLUDED_REGION=51822, 171 SEQUENCE_EXCLUDED_REGION=51999, 280 SEQUENCE_EXCLUDED_REGION=52294, 284 SEQUENCE_EXCLUDED_REGION=52581, 132 SEQUENCE_EXCLUDED_REGION=53675, 287 SEQUENCE_EXCLUDED_REGION=54157, 128 SEQUENCE_EXCLUDED_REGION=54796, 1260 SEQUENCE_EXCLUDED_REGION=54910, 466 SEQUENCE_EXCLUDED_REGION=56696, 288 SEQUENCE_EXCLUDED_REGION=57124, 275 SEQUENCE_EXCLUDED_REGION=57404, 166 SEQUENCE_EXCLUDED_REGION=0,58 SEQUENCE_EXCLUDED_REGION=5975,51652 PRIMER_OPT_TM=65 PRIMER_MIN_TM=60 PRIMER_MAX_TM=70 SEQUENCE_TEMPLATE=CAAAGTTCTGGGATTACAGGCGTGAGCCACTGCGCCCGGCGTAGGCCAGTTTCTATACTGTGGAACATTAAGTGGACTTGTCTGCTTTTCCAAACTTTGGCGCCACTAGCAAGGTGCAGAGGCTTTGTGGTAAAGGAAGAGAAAGAAGGCAGAAAGTTGGGGAAAAATATGATGGGAATGTGTAGAAAGAAGGTCGGAAGGCAGTACAGAAGACAGTACAGAGTTGGGGGGGGGGGATCATATGTTCCCAAAGCTTATTGGTGCAACTGGAATCAATCTGCACACTTCAACCTCTTGTCAAGACTGTGATGGAGTCGGAGGTATTTGCCCCGGGTAGGTGCACAAGACGCATGGTTGCAGATGTGGAAACAGAACAGAACAAAACCACCCACGTCTCTAATGTTTCAAATGAATGATATACATTTGTTTTTATTACTTATTTATTTATTTTTGAGACAGGGTCTCGCTCTGTCGCCTAGGCTGGAGTGCAGTGGCACAAGCTCGGCTGACTGCAACCTCCGCCTCCCGGATTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGTGCGCCACTACCACCCGGCTAATTTTTATATTTTTTAGTAGAGACAGGGTTTGCCATGTTGGCCAGGCTGGTCTCAAATTCGTGACCTCAAACGATCCACCCGCCTCAGCCTCCCTAAGTGCTGGGATTACAGGTGTGAGCCACCGTGCCCAGCCAAATAATATACTTTTAATAAATTTATTGACATTTAAATTTATTTTAAAACTTTATTTATTTATTTAGAGACAGGGTCTCGCTCTGTTACCCAGGCTGGAATGCAGTAGTGCCATCATAATCACTGCAGCCTCGAACCCCTGGGCTCAAGCGATCTTCCCACCTCAGTATCCCAAGTAGCTGGGACTACAGGAGCATGCCACCATGCCCAGCTTAAAAAGTTTTTTTGGCCGGGTGCGGTGGCTAATGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACACGGTGAGACCCCGTCTCTACTAAAAATACAAACAAATTAGCCGGGCGTGGTGGTGGGCGCCTGTAGTCCCAGCTATTTGGGAGGCTGAGGCAGGACAATCTCTTGAACCCCGGGTGGGGGGAGGTTGCAGTGAGCCGAGATCGCGCCACTCTCCTCCAGCCTGGGCAACAGAGTGAGACTCCCATCTCAAAAAAAAAAAAATTGATTTTTGTGTGTGTTTTACAAAGTTCAAGTTAGTATGTGCGTATAATATGAGCAAATATACATATGCTGAGGGTTGTTACAAGGGTTTGATGTGTGGATGATCAAAAACATTTGCAGCCATAGCTCTCAGCCATTGTTTAGCCTCCAGGGATCACTAAGGCAGAAGCGATTTGTGTGTTTGTACTTGGTGTTCTGGAAAGGTTGTTCTGTCCCTTTCAAATGGGGTTGAAGGGTCCTGATGGAAGTAGACAGTATTTAGACTAGGACAGGTGAAAGGGCTGGACTAGGCACATGCTGGGGCTGAAGATTGCAATTTGGAAAACGGCAGTGAGGGTAGGGATTGTCTGAGGATGGGGTAAAATTGGCCAGAGGGAGAAAGGAGTCAGCTGGAAAAGCCAAAGAAGGAAAACCCAAAAAGTTGTGTCCTTCTTGAAGGAAAAGGGAATTTTTGTATTCCACTTGCCTGTGCTGTTGGGCCTGCCTGACACTGTCAGTAAAATTCACCTGGAACTAGCCACTTGGCAGCCACCAATTCTTGTTTTTGCCAGGTGTCAAGGAGAGCAAATGCAGTCAGAAGACTGCTGTCTGTTATTGTCAATCACTTCAGCCTTCCCTGAAAAGCCCAAGTGGGCAGGTGGGATATCTGACATGAAGAATTTGGTGGCTTTGTCTGCAGGAAAAGGGAATAGCAAAATTAAAGGCAAGACAATGTTCCTAGTTGGTTTAGGGAATCAGTAATTTACCCCAGGGTGCGGGTGGCTCACGCCTGTTATCTCAGCACTTTGGGAGACCGAGGCGGGTGGATCACTTGAGCTCAGGAGTTCAAGACCAGCCTGGGCAACATGGCAAAACCCCATCTCTACAAAAAATACAAAAATTAGCCTGGCGTGGTGGCATGCGCCTGTACTCCCAGCTACTCGGAAGGCTGACGTGGGAGGGTCGCTTGAACCCCGGGAGATGGAGGCTGCAGTGAGCTGAGATGGCACCACTGCACTCCAGCCTGGGCAACAAAGTGAGACCTTGACTCAAAATAATAATAATAATAATAGTAATAATAATAATAATTTACCCTAGAAGAGAAGGAGGAAGAGGTGAGGATATCTAGTGCCCAAAGAGAAGTCATCTGGACCGAACAAACAGATGGAACAAATGATAGCAACAGCTGAAACAACATCAACACATTTCAGATGGGAGGCCTGCCAGGTATTATGTAATAAGGTCACAGTGGTGACCCTGAGAGGCATGCCTACCTCTGCTCTTGAGGCAGGGGCCTCTAGAAACAGGATTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTCACTCTATCGCCCGGGCTGGAGTGCAGTGGCACAGTCTTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGTGATTCTCCTACCTCAGCCTCCCAAGTAGCTGGGATTACAGGTATGTACCACCACACCCAGCTAAGTTTTGTATTTTTAGTAGAGGTGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGACCTCCCAAAGTGCTGGGATTACAGGCATAAGCCACCGTGCCTGGCCCAGAAACAGTATTTGATAAATAAAGAGGCCAGGTGAGGTGGCTCATACCTGTAATCCCAGCATTTTGGGAGGCTGAGGAGGGAAGATTGCTTGAGCCCAGGAGTTTGAGACCAGCCCGGGCATCATGGGAAGACCCTATTTCTACATAAACAGATTTTTAAAAAAATTTAAAAAGGCATGGAAGGCATATGCTAGTGTAAGTATGAGGTACATTGAGTATCCACAGGGAAGTGTAATGACACCTGTCACTTTTGCCTGCCCAGCATCAGGTTGCAGCACCTTAGTTTTCTCTGGGGGAACCATCCCTCCTCCACTCTCAGTACACATGGTTTGCATGTGGATGACCCCAACGCTGGGCCCTGGAGTGGGCACATAACCAGGTATAACCAGCCAGCCACTCAGAGTGGGCAGACATCATGCCATAATCCAGTCTGGACCAGTGAGGGTTAGTCCCCAGGAACTTGTACTGAAGTATTGGGAATGCCATCCTAAGTCACAGAGAGGGAGACTAAAAGAAAATGGTATTTATTTGGAATGGGTACTGCAATGGGAATGTGTGTGCCATAGTAAACTGTGTATATTCAGGGAGGCGAAGGGAGAAAAAGGGTTTTGTTGTTGTTGTTTTTTAAGGTAGGGTCTCACTCTGTTGCCCAGGGTGGAGTGCAGTGGTGTGATACTGGCTAACTGCAGCCTTCACCTCCTGGACTCGGGTGACCCTTCCACTTCAGCCCCCTCGAGTAGCTGGGACTACAGGCATGTGCCACCATGCCTGGCTAATTTTTTTGCATTTTTTGTAGAGATGGGGTTTAACCATGTTGCCTTACACTGGTCCCAAACTCCTGGGCTTAAGCAATTCTCCTGTCTCGGCCTTCCAAAGTGCTGGGATTACAAACATGAGCCATTGCAGCAGGCTGACAAAGGTGTTTTTTGTTTGTTTGTTTGTTTGTTTGTTTGTCTGTTTGTTTGTTTTTGAGATGGAGTTTGGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGCGATCTCAGCTCACTGCACCCTCCGCCTCCTGGATTCAGGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACGGGCACCCGCCACCACGCCCGGCTGATTTTGTATTTTTAGTAGAGACGGGGTTTCACTATGTTGGTCAGACTGGTCTTGAACTCCCGACCTCAGGTGATCCGCCTTCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAACCACTGTGCCCGGCTGAGAAAAGTTTTTAAAGAAAAAATGAGAATTACATAATTGTTTTCAGGTAATTATTCTTGGCTACAAGGATTAATAACAAAGGTGGCATCAGTCCAAATTTGGACAGGCAGCTGCTGGGTAGATGTGTTCACAGAAGTATTTTCTGTGTGTAAGGTGATGAGACTGCAAAAACTATATCACTCTGGTGATGATAGCCGTTGTACAGGGTTATAGTTTTTGCAGTCTCATGATGGGTTTTGCTATCAGGCATCACACATGAGAACCTTCCCTTCATGGCCTTCCCCAGCTCCGGTTTTCGGGTTTTTTTTTTTTTTTTAAGGTGGGGTCTTGCTATGTGACCCTGGCTGGAAGTGCAGTGGCTTTTCACGGAGCGATCCTACTACTGATCAGCATGGGAGTTTTTATTTGCTCTGTTTCTGAGCTGGGCTGGTTCACTCTTCCTTAGGAAACCTGGTGGTCTCCTGCTCCCAGGAGATTACCATATTGATGCTGAGTTTAGTGGAGACACCAGATCAGAATAGCATACCACAGCCCAGAAATCCTGGGCTCAAGTGGTCCTCCTGCCTCAGCCTCCCCAGTAGCTAGGAATACAGGTGTGTACTACCATGCCTGGCTAATTTTTAAAAATTTTTCGTAGAGACAGGGTCTCCCTATGTTGCTCAGGCTCATCTCGAACTCCTAGGCTCAAGGGATCCTCCCATGACAGCCTCCCAAAGTGCTAGACTACAGGGGTGAGCCACCGCACCTGGCCTAGCATGGAAGTTTTAACCTGCTCCATTTCCAGCCTGGGCTGGTTCATTCCTCCCTAGACAACCTAGTGGTCCTCCATTCCCAGGATACTGATGCTGAGCTTTTTGTGGACACCTGATCGGCCTGGCATACTACAGCCCAGAACTCCTGGGCTCAAACAATTCTCCTGCTGCAGCCTCCTGAGTAGCTAGGACTACAGGCATGTGCCACCACACCTGGCAGGGTTTGTTTGTTTTTTTTTTTTTTTAATGAGACAGAGTCTCACTTTGTCACCCAGGCTGGAGTGCAGTGGTGCGATCTTGGCTCACTGCAACCTCCGTCTCCCAGGTTCCAGCAATTCTCCCACCTCAACCTCCTGAGTAGCTGGGACTACAGGCCTGCATCACCATGCCTGGCTAATTTTTGTATTTTTACTAGAGACAGGGTTTTACCATGTTGGCCAGGATGGTCTCAAACTCCTGACCTCAAGTGATCCACCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCACCTGTTTTGTTTTTAATACAGGTGACTCCATTTTGCTTCTGGCAACTTTCACATTTCCCCTTTTTGATCGTGATCTTTCTTCAAAAGCAGTAGGTTTGGATTGTCCCTCAGTGCTGGGATGGACCTGTCCTGGGTTGTTGGTCTGGTTCCAGATAAGAGGGAGTGATTGACAACTAAGAGTCAGTGTCAAAACCCCTTTAGGCACATCTGAGCAACAGTGGAAATTTGGAGAGAGTTGCACTCAGACTAAGTCTACCTGAAGTCCATCGTTAAATTCTATTTTTTCTGTTCTGTGGTCTTTTGGCCATCATCTGGAAGCCCCGTGCCAGCATACTCTGTTAGCAGCTGTACTTCTGCAGAAGTTTCACAAGTAACAAGTACAAATTGTTAAAAGGAAAATACAAATTAATATTAGTAGTAATATGATACCCCCAGTTTTCATAATAGTTTTGAGCTATGAACCTAGGCTTAAAGGCAATCAAGTGAATAAATCAAATGACCATGGGGAATTAGGTGAGACCCATTATAACCTATGTGACCTGTTATAACCTATGTTTTGTAATTTCGTGTATATGGGGCCTCAAGTTCCCCAGGGGAATTTATCCAGGTTCAGCATGTGCTATTAGCAATAGAACAGATATTTCCTGATTTAACCAATAGATGCTGAAGGATCTCTTAGGTCAGGTTCTGTGAGGTTACTGACAGAAGCTATTGATTGTGCAATTTCAATTACACCATTATCCTGTCAACGAAAAGGCAGACATAAGAAAGGAAAAATTAAGAGTGACAAGATTTTCATTTTGATGGGTGAAGCTCTCAACTTCTTTTTTTTTTGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGCAGCATGATCTTGGCTTACTGCAACCTCCAACTCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGCGTGCCACCACAGCCAGCTAATTTTTAAAAAATATTTTTGGTAGAGATGGGTTTCACCATGTTGGCCAGACTGGTCTTGAACTCCTGACCCCCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATGACAGGCCTGAAGCCACCGCGCCCAGCTGAAGCGCTCAACTTCTTGTTCTGGTTTGCAGTTTGAGTGTCTCTGGTTATGGCATCAGGTGGTTTGGAGAACTTCCTGTGTGACCCATACATCAGGCATGAGACTTGTCCCTGGATATTTATACCAAGTTTTCCAGCTTCAGCTTCAAGGCCTTTAGCAACATAACAGGTTTTTTTCTTAGTTGGAGAGTTTTAGCCAAATATTAGAGGAAATTAGGAGGATTTGGGGTCTAGTCCCGTCTTCATGTAGATAAGAAACAATGCAAAGGGCTGCAATCTAATAGCAGGCATATTTTAGTGTTTTTCCTTTAGAAACAACTTTTTCTGGGAGGCCAAGACGGGCGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAAAAAAAAAAAAAAAAAAATAGCCAGGCGTGGTGGCACGCACCTGTAGTCCCAGCTACAGATTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCAATCTCAGCTCACTGCAACCCCCACCTCCCAGGTTGAAGCAACACTCATGCTTCCACCTCCCAAGTAGCTGGGATTACAAGCATGAGCCACTGTTTTTGAGACAGGGTCTCACTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCCATCATGGCTCACTACAGTCTCAACCTCCTGGGCTCAAGCAATCCTCTCCTCCTACCTGAGCTCCCTGAGTAGCTGGGCATACAGGTGCGCACTACCACACTGGCTATTTTTTTTTTTTTTGTGGTAGAGATGGGGGTCTCCTTATGTTGCCCAGGCTAGTTTTTTTTTTTTTTTTTTTAACTTGGGTACCTTTGGTATTTTTAGTAGAGACTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGGCAGGCTGGTCTCAAACTCCTGACCTCGTGATCCGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAAGCACCGCGCCCCGCCTGCCCAGGCTAGTTTTAGAACTCCTGGGCTTAAGTCATCACCTGTCTCAGCCTTCTAAAGTGCTGAGATTACAGGCGTGACTCACCATGCCCACCTAATTACTATATTTTTGATAAAATAATTAATGCTTTTAATTTTTCCCTTTAATTAATTAGATATTTTTCATATATTTTGGTAGAAAATATTACACAGAATGTGAAAACGTACGGACACATGATTACATAGAAACAGATTCCCTAGCTTTCATTTTGAAATTTTTGTCATGAGACAGTACAATATAGTAATATACGCTTGCCAGTTTATAAAAGGACAGTTGGATCCAATTTCTGACAAAATGAGACCCGTTCGTGTGGCTGAGCATTATTTGACCCCATAGGTAATCTTATGAAGGCTGAAGATCAAAATTTTGGGTAAAGCAGCGTCTACAGCAGTTTGATTTAAAATATCTTTTTAAAATCCTTTTATTCCATATCAAATGAGTTTAGAGGTTAAATATTCAAATGTTCACATTTCAGTTAGGACTAGCTGAATTGTATGAGAAAAACAGAATTTCCAGTGGCCAATCTGGTTTGCTTGATTAGTCAGCACAGGTGGAGAGGCACTTTTCAAAAAGATATTTACAGTTGTTTTTTTTCCTCAGCTTTTTCTGGATTGTACATGACAGACAAAGCCAAATTTTTATGCTGGACAGAGATACCTTATGTGATTGCTGTGTGCTCAAGAGTTTGACCTGTTTGATCTGAGAACCTAACTTTCACAAACATTGATCTAGTTCTTTCCTTTTTAGACTATCAATCTTTTAATTAACTGTTCCGTCACCCTAAGCAATTGTCAGCTAGGCAAACCTCCATACATGTTTCTGAAAGGAATGACTCCTAAGTGTACAAGGCTAGGTTTTTGGTTGCCATGGAGCTGTTGTAATTTGAAAGCACCCTTTTTTCTTTTCTTTTTCTTGGCTGAAATGCCCTAAGGAAAAGTTATAAACAGCTTTTGAAATCACCAATGTTAGATTATCTTTTTTTATTCTTATTTTTTTGAGACAAGATCTCACTCCATCGCCCAGGCTAGAGTGCAGTAGTGTGTCACGGCTCAACGCAGCCTTGAACTTCCAGGCTCAAGTGATCCTCCCACCTCAGCCTCCTGAATAGCTGGGACCACAGGTGTGTGCCTCCACGCTAGCTACCTTTTTGTATTTTTAATGTAGGGTTGGGGTCTGATCAGGTTGCCCAGGGTGAAATTATCTTAATACAATTATCTTAATACAAGTGAAGAAGCTGGCTGGGCACGGTGGCCACGCCTGTAATTCTAGCACTTTGAGAGGCCAAGATGAGTGGATCATGTGAGCTCAGGAGATAGAGACCAGCCTGAACAACATGATGAAACCCCATCTCTACAAAAATTAGCCAGGCGTGGTGGCAGGTGCCTGTGTTCCCAACTCCTTGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGGTTGAGGCTGCAGTGAGCTGAGACTGCACCACTGCACTCCAGCCTGGGTGGCAGAGTGAGACCCTGTCTCAAAAAAAAAAAAAAAAAAAAAGAAGAAAAAGGCCAGGTGCGGTGGTTCACACCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCAGATCATGAGGTCAGGAGATCGAGACCATCCTGGCCAACATGATAAAATACAAAAAATTAGCCAGGCATGGTGGCGCATGCCTGTAGTCCCAGCTACTCGGGACGCTAAGGCAGTGGAATTCCTTGAACCCGGGAGGCAGAGGTTGCCGAGATCGTGCCAGCCTGGTGACAGAGCAAGACTCCGCCTCAAAAAAAAAAAAAAAGAAGAAGAAGAAGAAAAAGCCAACAGAGTCAGCAGAGAGGAGGAAGAAAAGAAAAGCAGATAGAGAAGTTAGGCGCCTCTACATACCAGTGTTTTAGTTTTATTTATTTAATTTTATTTTTTATTTTAATTTTATTTTTTTGAAACAGGGTCTCACTCTGTTTCCCAGGCTGGCCTTGAATTCCTGGGCTCAAATAATCCTCCTGTCTTGGCCTCCAAAAGTGCTGGGATAACAGGCATGAGCCACCGTGCCCAGCCCCAATTCCTTTTAAAGGTGATTTTGTTTCGGGTCTCACTTATTTTTTTTTTCTTTTCTTTTCTTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTCTGAGATAGCCTTGACCCCTGGGCTCAAGCCATCCTCCCACCTCAGCCTCCCTAGTAGCTGGGACTACAGGTGTGCACCACCACACCTGGCTAATTAATTTTTTTTTTTTTTTTTTAGAGACAGGGTCTTGCTGTGTTGCCCAGGATGGTGTCAAATTCCTGGGCTCAAGTGATCCTCCTGCCTTGGACTCTCAAGTGCTGGGATGACAGGTGTGAGCCACCACGCCCAGCTGTAGGTCCCACTTCTGACTCTAGTTATGTCATCCTAAATAACAGGCTCTCTGAGAGAAAATGATATTTATTCTGGAATGCGTTGCAGTGGGAATGCATGTGCCATGGTAAACTGTATGGTAAACTGTGTGCATATACAGGGAGGCAAAAGAAGGCAAAGGTTTTTCAAGGAAAAGTGAAGAGGATCACCTAATTGTTTTGAGAGAATTCTCCTTGGCTACAAGGATCGATAACAAGGGTGACCTCAGTATGAGGTTGGACAGGGTTAGGGTTAAGTTGCTCAGCTGGGTGCGGTGGCTCACGCCTATAATCCTAGCACTTTGGGAGGCTGAGGCGGGTGGATCACCTGAGGTCAGGAGTTCAAGAGCAGCCTGGCCAACAAGGTAAAACCCTGTCTCTACTAAAAACACAAAAATGAGCCAGGCGTGGTAGCGCATGCCTGTGATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTAGGAGGCGGAGGTTGTGGTGAGCCAAGATTGTGCCACTATACTCCAGCCTGGGTGACTGATCCAGACTCCGTTTCAGAAAAAAAAAAAAAAAAAAGTCAGCCGGGCACGGTAGCTCACGCCTTTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACTTGAAGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCGTGTCTCTACTAAAAATACAAAAATTAGCTGGGTGTGGTGGTGCACACCTGCAATCCCAGCTACTTGGGAGGCTGAGGCAGAATTGCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCCGAGATTGCGCCACTGCGCTTCAGCCTGGGTAACAAGAGCGAAACTCCGTCTCAAAAAGTTGCTGGGCAGATGTCCTTGCAGTATTTTTTTTCTGTGGAAAGCTGTGGTTTCTGCATTTGCAGCCTTCCCCAGCTCTGTTTTTTGTTTTTTAACACAAGTGACTCCATTTTGATTCTGACAACTGTCATGGCAAGGAAGGCTGCCATGTTGGAAAATGAAGCCAATACAGAGAGAAAAACAAAGCCAAGAGGTGGAGAGAGCCCAAGTCCCTTGGGCTTCGGGGTCCATTACAGGAACAATGCAATTCTGCCATCCTCCCTTTTTTTTTTTTTTTTTTTTTGCTTATGCCAGTTTGAGTGTTGAATTCTGGCATTGTAAGTGAGGACATCCTAACTCCTAAAGGGCAGGCCCAGGGGGCGATACCCCTGGCAAAACCAGGACCGTGGGATATGTCAAGCTTACCTCCTCTTTGGGAAGTCAGGCAGGCTGTGCTCCATGCCATCAGCTTCCTAGCCTGTGTTGGGCTGTGACACTGGTGACCCCAGTGAGGGTGCAGCCCCATGAAGAGGTAGAGGCTGTCACTGTGGCTCACTACGGTGTTCCTGGACCAGTTCAGGCACTTGGTGAATGTTTAGTGAACAAATGAGCTGAAGGAACAAATGGAGGAAGGCTGGGCAATCACAGCAGTTGCTGGAGAAGTGTCGGGGAGCCCAAGCCCCTCCCAGCCTGGCAGTATAGACACTCCGTGAGTTTCCGTTTCATTTAGGGGCAAGCTAGTAACCTCGTGGAATTGTACTTATCTGGCTTGTAGTCTTGCTACACCGGGAACTCTCCTATCCCCACTAGGGACCTTTACTTTTTTGTACAGTGGGGACAATGGCCACACTTACTGGCTAGGGTTGTGGTGAGGTGTTACCTATGGTATAACTGAGGTATTGATGCAAGAAGGAAGTTGAGATTTGTATCAACATATGGGACCATGTGGGTACAGATGAGACAGCACTGGTCACATGTCGATCACTAGAGGGATGAGCACATCAGAGTTGATGACAGTCATCTGTTGGTATCCGATAGGTATTTGTTCCAGGGCACCCTATAGCTGCCAAAGTCCAGGGATGCTCAAGTCCCTGATATAAAATGGATACATTATAGTTATACATATTTATTCCCATAGATTTTTTTTTCTTTTGAGATGGAGTCTCGCTCTGTCACCCAGGCTGGAGGGCAGTGGTGCAATCTCAGCTCACTGTAAGCTCCGCCTCCCGGGTTCAAGCGATTCTCCTGCTTCAGCCTCCCGAGTAACTGGGACTACAGGCACCCGCCACCATGCTCAGCTAATTTTTCGTATTTTTAGTAGAGACGGGGTTTCACCGTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCTTGATCTCCTGACCTTGTGATCCGCCCACCTCGGCCTCTCAAAGTGCTGGGATTACAGGCGTGAGCCACTGTGCGCCCAGCCCTTTTTTCTTTTTTTTTGAGACAGTGTGTCACTCTGTCGTCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAACCTCCACCTCCCAGGTTCAAGTGATTCTGCCTCAGCCTCCCGAGTAGCTGGGATCACAGGCATGCGCCAGCACGCCTGGCTAATTTTTGTGTTTTTAGTAGAGACAGGGTTTTACCATGTTGGCTAGGCTGGTCTGAAACTCCCGACTTCAGATTATCTGCCCACCTCGGTCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGTGCGCGGCCTGATTTTTTTTTTTTTTTTTTTTTGAGACAAAGTCTCGCTCTGTCACCCTGGCTGGAGTGCAGTGGCACAATCACGGCTCACTGCAGCCTCAACCTCCTGGACTGCAGCAATCTGCCTGCTTCAACCTCTGTAAGTGCTGGGATTACAGACATGAGCCACCAAGCCTGGTCCCATAGAATCTAAACCATCTTGAGATTATTTATCATGCTTAATACAATGTAAATGCTCTGCAAATAACAATTACACTGTATTGTGTGTGTGTTTTTTTTTGATAGGATCTCACTCTGTTGCCCAGGCTGAAGTGCAGTGGTGTGATCTTGGCTCACTGCATCCTTGACCTCCTGGGCTCAAGTAATCCTGTCTCAGTCTCTCAAGTAGCTGGGACTACAGATGTGTGTCACCACGACTGGCTAATTTTATTTTTTTGTAGAGATGGGGTCTCGCTATTTTGCCTAGGCTGGTCTTGAATGCTTGGGCTCAAGTGATCTGCCCACTTTGGCCTCCCGAAGGCTGGGTTATAGGGCATGAGCCACCGTGCCAGCCTACACTGTATTTTTTTTTTTTTTTTTAAGACGAAGTTTTGCTTTTGTTGCCCAGGCTGGAGTGCAATGGCACAATCTTGGCTCACTGCAACTTCTGCCTCCTGAGTTCAAGTGATTCTCCTGCCTCGGCCTCCCAAGTAGCTGGGATTATAGGCGCCCGCCACCACGCCTGGCTAATTTTTTTTTTTTTGAGACGGGAGTCACGCTCTGTCACCCAGGCTGGAGTGCAGTCATGTGATCTCAGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCGCCACCACGCCCGGCTAATTTTTTGTATTTCTTAGTAGAGACGAGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAGGCGTGAGCCACCACGCCCGGCCACTGTATTGTTTTTTACTTGTACTATTTTTTATTCTTGTATTTTTATTTTTTCCAAGTATTTCCAATCTGTGGTTGGTTGAATCTGTGGACATGGAACCCCCATGGATATGAAGGGCCGATTGTATGCTAGTCTGTCTCCTGGAGATGTTTGCAATTTTCCATAGCAAGTCTAAAGAGTGAAGTGTGTATGATCCTGCAGTGGATCCATCATTACACATTTTTGTCCAAACCCACTGAACGTCCAACAGCAAACTGAAGCCTCATGTGAGCACCGATACTGGTTCATTGACAGCAACAGATGGGAAGCTGGGGGCTGGCAGGGGGCACACAGGAACTCTGACCTTTTCACTCAGTTTTGCTGTGAACCTAAAATGGCTCTAAGAAGGAAAGTTTACAAAAACAAAAAAGGAATGGAATACACGTAGAAAGTTCCTGGTGCAGAGGCAGGACTGGCATCACCATCGTTTCCTCCCTGGTGAGCCGCTCCTCCAGGGCACCCCTGTGCCTCCAACCCCACATGTGCCTGCTCCAGAAGCACTTTTGTCTTCTGAGGCTCTGTCTGCGCTCTGTACTCTTCCTGGGATTGGCTCCTAAGTCCATCGCCCCTAACGTCGCCCCATGAAGGACACCACAGTGCCTCCCATCTAGGAACTGCCTTCTCTCCTTTCGCTGAACTCAGACTGCTTCTGGGCCTCTGGAGTTGATATTCCAGCAAAATTGCTTCTAATTTGCTGGCTCTCGCTTAGCAAAGGGAGGTATTAACCGTAACCTGGGAGAGACGCAAAAAGCCCAAGTGACTCGACCCCTCACAACCCTGACTTCAAAGGCAGCCGAAGTCTGTGGGCGACATGTGCTCCTGGATGATGATGGGGTGTGGTGGGGGCCTGGGCTGGTGTTAATCCCAATCCCTGTCCCTGTCCGTACAGGCCTGCACTGGGTCAGAGGGAGTTGTTGAGTCAGCTTCCTGCAGCCCGAGTGTGCAATCTTCTCTGCCAGACGAAAAGAGTCCCACTGCTCTGTTGACTGCTTGAGTCACATCTCAGTCGCAGCGGGGCCTGTTGGTGGCAGGAGCCCTGGAGACAAAAGGCTGTCTGCTCGGGCTGTGGGAGCAGGTTGGTTAAGACTCTGGAATCTGTCCTCCCGCACCCCAGAGGACACCGCCACCTCTGACTCCGCACTGACAACAGCATCTGAAGGCATTTCTTCTGTGGGGCCATCACTTTATTAAGGGGTCATCTAGAAGGTGGGCCCCCTGACAAACCGCGGGACTGTGATCGGGCTCCAGCTACTTCACCACCCCGGGCCAGCCTGCTCCAGGGGTCCCTTCCTGCTGAGAGCAGGCGAGAGGCAGTCAGGCTCATGAAGCAGCCACCGGGTTTGGCTCACTGGAAGGAATCACACTGGAAACATGTTTAGCCCGCAGTGCAGAGTGGCTCCAGAAGGGAGAGGTTCTGGAAGACGCCCCAACCTGCCGGGCTGCTCCCAGAGATGCACAGTGAGGGGCAGGCACCCAGGGCCGTTCCAGGACTCAAGATGGGGATGGAGATGGCGTGAGGAATGGAGGACAGGGCTAGATGGGCTGACCGGGGGGAACAGTGTTACGAAAAGGAGGCGGGTACCCTGGGCTCCCGTGACAAAGTGCGGCAGGGCTACCCCCTGCAGCCCCCATAGCCCCCACCACCTTGAGACCACGTTCTGGTCACTGCCTCGGAGCCCCGATGTGTTGGGGCCAGGGAGCGCTCCTGCCCGGGTGTGGGGTGTGAGCCTCAGCCTCTGTCCGCCCGGCAGCGCGCGTGCCTCCCTTGGTCTGGCCTCCCTCGGCCCGGTCCTGGCACTGGCCGTGCTAGCTGGCCGTGCTTCTCCGCGGGATACAGCCTTCCATCTCCAGCGCCTCGGGGCAGCCTTCGTACTTGTTGCTGTGCTTACTGTTCTTCACGTGCTGTGGGGAGGGGAGCAGAAAGTCATGACTTTGGCCCTCAGGGACAGGGGACAGGAGTGGACCAGGACCCAGGCTGTCAGTCAACTGTGCTGTGTTGGCCAACGGTGTACTCTCAGGGCACTTGGCCCCTCCACCCATCCCAGGGAAACATGGCATCAGCTGCACTGGCCCACCCCTCATAGCTGGACTGGGGACGGTCCCCTGCTGGGGCGTGAGCAGCTGGGGAGGCTCGTCGCATGCAGGCAGAAGGGGCGACAGGCTGCCCCGGTGGGGGTGATGCCAGGCAACCCCCCATACCTGCTCAAAGGGGCTCTTGTTCTGCACACCCTTGGCCCCGACAAACACCCAGCTGTCCCGGAAGGCCAGCTCCTTGGCGTTCCTGCTGCCCAGCTCACTGAAGAGCTTTCTGGTCTCTTCATTCATCCTGCAGCATGGGAGGAAGGGGTGTCAGCTGTTGCTGCAGAAGGGCAAGGCTGAGGCTGGCCTCCCCAAGCACCTACTTGGTGGCTGGGTCGTCGTAGGATGCCACGAACACCAGGGTGCCTTCGTGCAGTGGCCGAATAAACTTCAACAGGTCGTTGACATCTGGGGGGGCAGGTGCCACGGAACAGGGGTCATCAGGCACCACTGAGCACCCTCCCACAAGCCCGTTCTCCAAACAAGGAAACAGAAGTAGGGATTGGGCCTGGGACATGCCCCGCCAAGGGACAGGCTGGTGTAGGAAGCTGAGGCATCCCCGTGACCTCCCACAGCCTCCAGACCAAGCTGGCGATGCCCCTGTCCTTTCTGAACCCACGGAGGTGACTGAGAAGCTCCAAGTACAGACAGAGAGAGCAGTGTCTCAGGGGAAGCTGCCCAGGTCATTTTGCTGCAGTCAAAATGCAAGACGCTGCGCTGCAACTCACCTCCGGCCCACATGTCAAAGGCCCGGGCCTCGATGAGCTCGCCGCTGACCCCTGTGTCAGGAGGGAGGGGCCCTGCTGGGTAAGCCAGGCCAGCCTCAGGGCACGGCAGGCGGGTAGCACCGGGGGTGGGGGGCAGCCCTTCTGGACGCTTTCAAACAGTCCCCCAATATTCATCCATTTGTTCAGGCAGCTGCATCCCACCCCAAGCCGTGGTGCCTCTGTCTGGCATTTGAGGCCCTCCGGGGGCTCTCCATGAAGCACCCTCAACCTCCCAGAACCTTCCCTAGTTCTTTATTGCCATCATGCTTTTTTGTTCATTGGGTCACTCCTCTGTGGAGCCTTGCGCTTGCACAGACTCCTTCCCAGGCCTCCCTGGGAGATCTTGAATGGGAGGGGAAGGGAGGGCAAACACCCAGAGGCCTCCTGGGGAGGGAGCAGGGAAGGAGGGCGGCAGGCCACGCTCAGCCAGGGGCCTCACCGTTCACCAGGGCGATGTTCAGCCCGCGGCCCACGTTGTCCTTGACGCTGCTCATCAGCCTAGTTGGGGGGGGGTGGGGGGGACGGGGAGATCCCACATGGGCACGTCTGCTCCACTCTCCCTTGCAGACCCGGCCCTGGATCCCCCACTCCCTCCCTGATCCCCAGTCACCCCAGGGAGCTCACATCTTGTCCTCGAGGCAGATCTTGGGCCCAATGACGTTGGCGGCCCCGCTGACCACGCGGAAGGCCAGGTGCTCCTCAGGACACGGCTGGGGCAGGCCACACTTGTACTTCCTGGCCCGTGGCGCTGGGCAGGGATAGCAGGTGTTATCCATGGGCCTGGCCCTGAGGTCACCTGAGATCTGAAGGGGAGGTCAGGACCAGCACACATGGTAGAACAAGTGACCGCAGAGCAGGACACAAGTAGGGGGATGTGGCTCAGGCCGAGGCTGGGGACACTTCCATACTCTGTGTCCTACTGGTGAGGCCACAGGGACCCAAGACAGAAGGATTTTCTGTTACTGGTGGGAGAAAAGAAAGGATGCTGAGGGCTGGCCTGGGCCACTGCACAGCCAGGAACAAAGCAGGGGTGAAGTAAGTCAGGCTGGGGGTCTAGCTGCATGGATGAGGCCAACCTGAGCAAAGGCAGTGTGGCAGGGGGTAGTGTGGCAGGGGGTGCAGCCTGTCTCCCGATTTTGATGGGGGTTGGTTGGGAGGACTGGAGCAGGATGGCTTCTATTATGGGTTGAATTCTGTCCCCTCAAGGTATGCTGAAGTTCTAATCTGTGGTACTTCAGAATGTGATGTTTGCAAATAGGGCCTTTGCAGTTCAGATGGGGTCATACTGGAGTAGGTTGGGTCCTTAATGCAACATGACTGATGTCCTTATAAGAACAGAAGTGACAAAGACACACAGAGGGAAGACAGCCATGGTACAGTGGAGGAAGAGACAGGAGTGACACAGCCAGAGGCCAAGGTATGCCTGGGGCCACTGGAACCTGGGGAGGTCAGGAAGGACCCTCCTGTAGAACTTTCAGGGTGAGTAACGCCCTGCTGACACTTTGATTTCAGACTTCTGGCTTTCAGAATGGGGAGAGAATCCATTTCTGTTGTTTAAGCCACCCAGTTGAAATGGCAGTTCCAGGAAATGGATACAGGTTTTGGCACCAGGAAGTGTGGTGCTTAGGTAACAAATACCTAAAAATGTGGAAATGGCTTTGGAACTGGGTAGTCAGTAGAGGCTTGTCTTAGTCCATTTGCTGCTGCCATAACAGAATACCACAGACTGGGTAATTTATAAAGACAACAGATTCATTTGGCTCATGGTTCTGGAGGCTGGGAAGTCCAAAAGCATGGCGCCGGCATCTGGTGAGGGTCATCCCGTGGCAGAAAGGTGGAAGGTGGAAGCAAGCATATGAGACGCAGGAAAAAAGGGGGCTGAACTTCAAGGTAACTAACTCACCCATAATAATGGCATTCATCCATTCATGAGGGCAGAGCCCTCATGACGTAACTACCTCAAAAAGGCCCTCCTCTCAATACCAATACATTGACAAATTTCAACATGAGTTTTAGAGATGACATTTAAACCATATCAAGGCTATAGAAGTTTGAGGTACATGTTAGAAAAGGTCTGGATTGCCTTTAAGAGACTGTTGGTAGGAGTATGGATGTTAAAGGTAATTCTGGTGAGAGGAGGGGAGAGCAATAGAGACAGCCTCTGTGGTCTTAGAGAATACAAATATCATCAAGAACAGAACGTTGCTAGAAACATGAATGTTAAAGGTGCTTTTGGGTTGGGTGTGGTGATTCACGCATGTGATCCCAGCACCTTGGGAGGCCAAGGCAGGAGCATCACTTGAGGCCAGGTGATCAAGACCAGCCTGGGCAACATAGTGAGACCCCGTCTCTACAAAAAATACAAAATAAGCTGGGTGTGGTGGTACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAGCTATGATTGCGCCACTGCCCTCCAGCCTGGGTGAGAGAGCAAGACCCTGTCTCAATACATTCAAAAAATAAATAATAAAAAATAAAACATTAGCTGGGTGTGGTGGCACATGCCTGTAGTCGCAGCTACTTGGGAGACTGAGGTGAGAGGATCGCTTGAGCCCAGGAGTTTGAGCTTACAGTGCACGGGGATGATACCACTGAACTCCAGCCTGAGCAACAGAGTAAGACCTCTGTCTCTACCAAAAAAAAAAAAAAAAAAAAAGGGTGCTTTTGGTTAAGCCTCAGAGGAACTGGAGGGACATGTTATTGAGTTATTGGGCGCTGGAGGAAAGCTGATCCTTGTTATAAAGGAACTTGGCCCAGGTGTGGTGGCTCACGCCTATAATCCCAGCACTTTGCGGGGCCAAGGCGGGAGGATCACTTGAGGTCAGGAATTCGAGACCAGCCTGACCAACACGGTGAAACCCCATCTCTACTAAATATGAAAATTAGTTGGGTATGGTGGCAGGTGCCTGTAATCCCAGCTAATCAGGAGGCTGAGGTGGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGATGGCTCCACTGCACTCCAGCCTGGGTGACAGAGCAAGACTCCGTCTCAAAAAAAAAAAAAAACTTGGTTGAATTGTGTTCTAGTGTTTTGTCGAAAGGAGAATGTGTAAGTGCTGGGTGCTGAACTTGGATATTCAGCTGAGGAGATTTCCAAGCAGTGTTGAGAGCGTAGCTGACTTCTCCTTGCTGCTTATAGTAAAATGTGAGAGACATAAGGAAGAAACTGTTGAGCACAAAGGAGCCAGAAAGCGAAGATGTGGAAAATTCTTGGCCTAATCATATTACAAAAACTGCAAAAGTGTCCCCTGGACAGAACAGCAAGGCTGTTCAAGCGTTTGCCCCAGAGCTGAGGTATGTGACTCGTGGGTCCACTCAACCCTTTCAGCAGAAATCTACAATGAAGATGGGGTTATCTAGGAAGCGTCTGTGGGAAAACTCTCTGATTTTATAGCTTGGGCCTCTATAACTTGCAAAAGAGACCAACAAGGCTCTTGAGAATCTGACACCAGCAGAAACACTGCCGGCCTGGAGTGAAGGGGGCAGAGATGAGATAAAGGGAAGGAAGGGTGGCCCCGAAGGTGGGGCTGTCTCGTTTCAGAGCATGGGTCATTCCAGCAGGCCCAGAAGAAAGAATCTCAGGCCACAGAGGATTCCCCTCAGGCCTTGAAACCAAATGAATTTTGCCCTGCTGGGCTTTGGACTTGCTTGGGACGGGTGACCTCTTTTTTCCTTCCAAGTTGCCCTTTTGCAGTGGGAGTATCTATCCTAGGCCTGCCCCACCGTCATATTCTGGCAGGAAGTAACTTGGCAGGAAGTAACTTGTTTTGTAGGTTGACAGTGGCAAGCAGCCCCCGTGGACCACCACAGCTGGTTTGGGGAGGAATAAATGGAAAACCGAAGCCCTACGGGACCGGGGGCAGGCAGGGATGTCACAAAGGGGAGGAGCAGGGAGGTGACAGGGGCCTTACCTGCAGTCACCGAGCTCTCTGGACCTGTGGATACAGAGTGGTTTCTGTTAGTGTGGAGCCTCCCGGGGCCTGCGGCAGGGCAGCCTGGCCCTCCTTACACCGCGAAGTGTAGACACAGGCAGTCAGGCTCAGGGGCCGGGGGCTAAGCCAGGCCGACCTTGGCATGCTTCCTGCTCAAGTGTAAGTCATGTTCAGATGAAAAACTCCCTACTCTAGGAACAGGGGGAAATGGAATTAGAAAAACTACTTAGATGACCTGGCCGGGCATAGTGGCTCATGCCTGTAGCACTTTGGGAGGCTGAGGCGGGTAGATTGCTTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACATGGTGAAACCCCATCTCTACTAAAAATACAATAATAATAATAATAATAATAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCCGGGTTTGGTGGTGCGCACCTGTAGTCTCAGCTACTCGGGAGGCTGGGGCACTAGAATCGCTTGAACCCAGGAGGTAGAGGTTGCAGTGAACCGAGATTGCACCACTGCACTCCAGCCTGGGAGACAAAGTGAGACACTGTCTCAAACAAACAAACAAAAAAGTACTCAGGTGACCTATTACCAGTCAACAAGGTGGAGAGTCCCCTCTCCATCTCAGAGCCTGCAGGGAGCAACCGGGGCATCACTCCCCATGTTCAGTGGAAGCTCCCTACCCCTTACTCTGGCCCTACTGACTCCAGTTGGGCATATAATTCTAGTGATCAGGTATTGGCTTTCCTAGATGAAGCAACTGGGTGTTGGGGGAGGCTGCGCCTGATGCCAGGAGACAGACTGGGGACAGCCAAAGGTAGCCTGCCAGGGAGGAGATAGAAAACCCAAGTCAACTCCCAACCCAGAGCCTCAACCACAGGCCCAGGTGGCCACCTTGGTGGCCTCCAGAGAAGGATAAGGCGGGGTCCCACACTCACTGGTGAAGAGTTGCTGGATGCGAGGAAAGCCACTGCCAGGCCCACCCAGGAGGATGCTGACCACGATCCATGTGACACCCACACTGACGACTAGGACCACAATGCGGAGAGGGCCTGGAGGCAGGATAGACACAGGGTGGGGTCACCTCAGATACCAGCGAACCCCAGAATGACCCCATAGCCTTGCATGACTGGATCTCCCCTGCCACTTGGGAAGAAGTGGCTGGAGGACAAGGAGGTGGAGACCTGTTGCCTTCTACCAGGGCTGAAAGGGAGACTTGAGCTCCCTGGCAGGTGGAAGGAGGAAATTGAGTTCAAATCCCAAGGCTGCTGGGTTTGGTTTTCCCTGGCGGGTCTGGGAGGGGGCCTTCCAGAACTCTCTGCTCGAGAAGGGCTCCAGCCCTCTTTCCAAGAACCCCCTCAACTTCCTGCCTCTGAAACCACCAATATATTTGGGCATCCACCTGCCCCTGCTTTCCTCCTGTTCTGAGGGAGAAGGGTCCCTACTCCCTTTTAAGACCAGTCCCGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGAACTTTAGGAGGCCGAGGCGGGTAGATCATGAGGTCAGGTGTTCGAGACCAGCCTGACAAAGATGGTGAAACCCTGCCTCTACAGAAAATACAAAAATTAGCCGGGCATGGTGGCGCGCACCTGTAATCCCAGCTACTCAGGAGGCTGAAGCAGGAGAATCAGTTGAACCTGGGTGGCGGAGGTTGCAGTTAGCTGAGACCATGCCACTGCACTCCAGCCTGGCGACAGAGTGAGACTCTGCCTCAAAACAAAAACAAAAACAAAACAAAACAAAACCAAAACAACAACAGTCCCGTCATCTGCTGGGACCTGGATTGCAAGCTCCCCAGCCTTCTCTGATTTCTTGTAATAGTGATCACTCTCTCTAGTTCGCTCTTTCTCCTGTATTTTCAACATCTCCAATGTTAAGGAAGCAAAGAAAACACTCTTCTTCAAAACCACATCCCCATCCTCTTTCCCCTAACCTTTCTTTTCCCTGTCAACCCCCTCAAAAGTCATCTATACTCATTCCATCATCTCACCTCCTACTCCCTCCCACCCTACTCCAGTCTGGCCTGGCTTCCACCACCTCACCAAATCAGCTTGTTGCCAAGGCCACTGATGACCTTTGTGGTGTCACCACCCCTGGTGACCTGTCAGCCTTGATCTTCCTTGACCTCTCAGTGGGCTGCCCATGTCCACCTGACAGAAGCACTAACTTCTCTGCTGGCTTTCCGGACAGCACACTCTCCTGACCCAATCTCTCTGGACACTCCTCATTCTCCTTAGTTAGATCGTCTCCTCTACCCTCCCCTTAAAAGCTGGACTTTCCCAGGCTCCCATCTCAGGCTGCCTTCTGGTCCCTACCTGTAGTGTCTTTTTATTTTTTTTATTTTTGAGACGGAGTCTTGCTCTTGCCCCCCAGGCTGGAGTGCAATGGCGCGATGTCGGCTCACTGCAACCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCGCGCACCACCGTGCCTGGCTAATTTTTTTTTTTGAGACAGAGTCTTGCTCTGTTGCCCAGACTGAAGTGCAGTGGCGTGATCTTGGCTCACTGCAAGCTCCGCCTCCCGGTTACACGCCATTCTCCTGCCTCAGCCTCCCTGGTAGCTGGGACTACAGGCGCCCGCCACCACGCCCGGCTAATTTTTTTGTATTTTTAGTAAAGACGGGGTTTCACCATGTTAGCCAGGATGGTCTTGATCTCCAGACCTCGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCCGGCCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATCTTGGCCAGACTGGTCTCGAACTCTTGACCTCAGGTGATCCACCCGCCTTGGCCTCCCAAAGTGTTGAGATTACAGGCGTGAGCCACTGCGCCCAGCTGTCTTTACTTTTTTGAGACGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTTATGCGGTGGCGCAATCTCGGCTCACTGCAACCTCAGCCTCCCGGGTAGCTGGGATTACAGGCACCCGCCACCACGCCAGGCTCATTTTTTTGTATTTTTTTAGTAGAGATGAGGATTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACTTCAAGTGATCCTCCCACCTCGGCCTCCCAAAGTGCTAGGATTACAGGCGTGAGCCACCGCGCCCGGCCTGTAGTGTGTTTTTAGATGATGTCATCTACTCCTAGCTCTTTAATTAAGCACCATGTGTCAATGACTTTCTATTTTCTAGTTCCAGCCGTGGCCTCTCTTGAAACCACTCCCTGCCAGCTGGTTCCTCAGTGGCCAGCTCCACCTGGATATGTCCACAAAGTTACCACCTTAGCAAGTCTGAAGTCCAATTCCCGAGTTGTTTCTACTCTCCACCCAGCCCCAACCTATCCCTCCCCAAGGATCTTCATCTTACTTGTCCAGGTGACAGTAGGGCTGTCATCGGGTTCATGTCGAAGCCCACAGCCACTTTTGTCACTTCCCCTCTAAGACCACGCCTCTCCACCTGGACTGCCCACCTCCTCTCTCTCACCACTCATTTTCCAAAAAGCAGCTGGTCTGCTACCTGCGTTGGGAGATACTGACTATACTAATCCTCATGTCATAGCTCGGGGAGCTGACGTCCTCAGAGGTGAAAGGATTTGGTTAAGGTCAAGGAACGAGTGTTTGTGCAGAGTCCTCCATTTCCAAAACCAAACCCAGTGGTTCACCCGGGCCTCTCCAGACACAGATGTCGCGACGCTGGGCCTCCAGCCAGGTGCCCTTTTCAACTCCCTCCCGTTCGCTGTGGAAGCCACCCACCTGCCAACCTCATGTCCACTTCTCCTGCTGGGTTGGGACCGCCGGCAAGTGCACTGTTTGGGGGCAAAGCGGAAGGACAGGTTTGGGTGGGGGTCAGGGGCAAGCCTGTGCGGGACCCCTGCTGGGGGCGGGCGCGATCGGTGCTACGACCTGTTTGTCCAGCCGCCCGGCCAGGCAGGGCTCCTCGGAAACGCGCGGGGAAACCCTGCCTGGCCAGGAGGGGCCTCAGGAACCCGTTGGCTCACGATCTTGCCCACAGGAGCCTCCGGGGCTGGGACGAAGATGTGGTTCTCCTGGGCTCGGGCCGTTCCTCCGGGCCTGGGGGCTGGCGATGCGGGCCGGGCCGGAGATTTCTCTGTCCGTGGGGAGACGTGGGCTCGGTCAGGGAGGTTCCCTGTCAGGCCCGCCGACCCGCTCCGCCCCCGGAGAGGACGCAAGGCCCCTGCGCAGGGTGGCCAGGCAGGCCCGGCAATGGCGGCTAAGGGCGGGGCCACAGCCCGCCGGCCCCGCCCCCGGCAGCAGCTGCGCCGTCTGGCTCCACGCAGGCCCCGCCTCCAGACCCGCCTCTACCGCTCGCGCTGGCCGCCGCATGGGAGGGGCCTGAACCTGTCCCGCCCCGAACCTGTCCCGCCCCTGGATCCCTCGTCGGCCCCACCTCCGCGGTGCGTTCAGGCTGAACCCACCGGCCGCCGAGTGGGAGGGGCCCACGTCGGTCCCGCCCCGGAAGTCTCCCCTCGGCCCTTTCTCGGGTGGGAGAGCCCTGCCTACCCAGGCTTAGAGGGCTGGGGCTGCGCTTGCACCCTGAGGTCAGATTTGTGAGTCGTGGGAAGACGCAGATGGATCTGGGGCAGAATTAGAACCTGTTCCAAAGTTGATCCATGCGCTTTGTAAAGGCAAGGGAGGTACAGAGGCGCAGATAGGGAAGACCGCAGGATCCAGCGTGAGCCTCTGGCTGCCTTTCCTTAGGGTCTGTTTTCTGTCCAGAAGCGCGTTCCTGCTGTCTCTCGTTATCACTTTTATAATGGGACGGGGCGTCTCAAACTATTTCGACATCCTTGAAAACTCTTGCCTGCTCCGCCTGCCTGGTCTCTAACACCCTCCTCCTCAGAGCTGCTCAAGCTGTGTTGTTTACGCCACACCCCACCCTAAAAGCAGAGGAGAATACAACTTTCGGGGGCAGTGGAGGGGGGAGTTTAGCGCTATAACCCGAGGCTTTCACAGCAAACCAGAATTTATTTTGAGGCCTCTCAGGTTCAGCTTTCAAGTCACGTGACTTTCGCGTCCTACACAACTCTTATTTTCACTTGAAAACAAGTGTGACGTCGTGAATTCCCTAACAATAGGAAAAAAGAAGGCTCCAGAAAGAGCGGTGCTTGGCTTTCCCAGCGGCTCCAAGCGGTGCCCGGCACCCAGCTGGCTGTATGGACCTGGGGTGGTCTCCGGCCGCAGGCAGACGCGGCCCTTCGGCCTTTCTCCAGTCACCCCAATTCTCCCTGACTGCCTGGCTATCTCCCCTCCGCGCCTGCCCGCACGCTGCCCCCAACACTGCCCGGGCTCCCTCTGCCCATGAGAACACGGGTCAAGCTCCCGTCCCTTCTGCCCTGCCGGCTGCCCCAAGTGCGCCCTGACCACCTACTGCGCTGGCATGGCCGGGGCCACCCTGACAGCTGATCCATCATCTGTCTGGAGAGCAGCAGCAGGGCCCAATGAGAACCACGGAAACCTCCCTGCCCTTTGACCCAACTTACGGGAATCTCCTACCCTTAGGGAAACAATGCAAAATATAGAGAAAGTTTATAGAGCCGGGCATGGGAGAGCCCTGGTTAGAATCCAGCAGCTTCTATACTGGAGTACTCAACAGGGTCAAGGTCCCTTTCAGCTGCCTGCTGCTGGGGCCAGGGCTTTGCATTTGCGTTTTGTCATGGAATTTGACAGCCTCTCAAGTAATTATGGTAACGAATCTTGGGCTCCATGCCCTCCTCAGGGGGGCCCAGTCGAAGAAGCTCCAGGTTACTCACACCCTTGAGCTGCCAGCCGGTGTTTTTCAAAGACAATATTTACATGTTGTTCAGAGCAGGCATTTATGAAAGTGTTGCTTTAATGAGTCCCCCTGAGCATTCCGAGACAACTCTCCCTTCCTTGGGGACACCACGTGGGTGATGGGCCAGAGGCCTGGGATTCAGTTCCACGTCTTCCGTGCTTCCTGGGGCAGCCTTGCAAAAGTCCCTTATTTTCTCTGGGATGGTTTGGATTCCTGTGGGGGTCTGGCCAGCACGGTGACTAGAGCACATGGTCTGTCACCATCCAGGCCAACGTCCTGGGGTACACTCACAGTCATCTGTTCTTCCAGGTCAGCCCTCATAGATCAGGGCAGCTTCAGCAGTTCTCAAATAGCAGATGTTACAAAATGGGGGTGGGCAATCCTGCAGCAGACGTCTCTGCACTTTCTGTTAGAAGGACACCCCAGGTATTTTTGAGCACTGTTCTGAAGACGGAAAAATCCAGAGTATACATACACTTAAAGCTGGGAGGGGCACCTGCCGGGGAGAATGCATAAGGGATAGAAACAAAAACAGAGGGCCGAAGCCTCTGCCAGGCAGAAGACACTGAGTTCTCAGCAAAGAGAACACCTTTCAGTGCTGTGGTCAAAGGGATGTCAAGCCTCCTGAGGCCCGGAAAGAGCAGGAAACTGAGGGCAGAATTGGAAAAAATCAAGTTACAGACACACACCACGGGATAACCTCTATACAGAGAGGAGCACACCCAAAATAATACTATATATTTATAGATGTACTTTTGTATTGGGGTAAGACATATATAACATAACATTTGCCATCCAAATCATTTTAAGTATACGATCCTGTGGCATCAGTTACATTCACAATGTTGTACAACCATCACTACTATCTTTTTGTGTGTGTGTATATTACTTTTTTTTTTTTTTCCCCGAGACAGAGTCTTGCTCTGTCTCCAGGCTGGAGTGCAGTGGCTCAATCTTGGCTCATTGCAACCTCTGCCTACCAGGTTCAAGCAATTCTCCTGCCTCAGTCTCCTAAGTAGCTGGGATTACAGGTGCCTGCCATCATACCCAGCTAATTTTTATATTTTTAGTAGAGACGGGGTTTCAACATGCTGGCCAGGCTCGTTTCAAACTCCTGAGCTCGTGTTCTGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGTGCCCGGCCATGTATTACTTTTATGGGCTCATATATATGTCCATAGAAATATAGAGTTCTTTTAAAAATTCTATCAATAGATATTTCTTTTTCTCTTTTCTTTTTTTTTTTTTTTTTTTGAGTCAGAATCTCACTCTGTTGCCCAGGCTGGAGTGCAGTTGCGTGATCTCGGCTCACTGCAACTCCTGCTTCCCGGGTTCAAGTGATTCTCCTGCATCAGCCTCCCGAGAAGCTGGAACTACAGGCGTCTGTCACCATACCCGGCTAATTTTTTTGTATTTTTAGTAGAGATGGGGTTTCACTAGTTGGCTAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAGGCATGTGCCACCATGCACGGAAATCAATAGATATTTCATTTCCACCAATACAGAAGCATCAGTTAACAGTGACTGGTTGACACTTTTAGAAAGTGGCTTGAGAAGGGCCCTGAATGAGCCACCTACGTGGAACACAGAATTTGAGATGTGCAAATGTGGAGACCTGAAGGCTTGTTATGGGAACAAAAGGTGGCTGTGTATAGAGAGGAGCGCAGATAAAGGGGACTTATCAGCAGTCGCAGGGAGTTCATGCTCAACAACTGGCAACAAGCGGGGCAGCTACAGGCTACTCAACTGGGGATTGATGCGCGGTGCCTCTGAGGTTCAGCTCTGGGCCTGATCGTCCAAGAAAGGGCAGCTAAGGTGGGCATAGTGAATCACGCCTGTAATCCTAGGACTTTGGGAGGCTGAGGTGGGAGGATTGCTTGAAGCCAGGAACTTGAGACCAGCCTGGGCAACAAAGTGAGACCCTGCATATATATATATATATATATATATATATTTACACAAATAAATAAAGGGCAGCTAGACTCCACAGAAACCATGTTGAAGATTCCTGTGCAGGAAGGGGGTGATAAAATGCAGAAACCTGACCTGACACAGCCAACACATAACTGCATTGCCTCATTTTTCACGATTTTTTTCTTTCTTTTTGGAGAGAGGGTCTCATTGTGTTGCCCAGGCTGGAGTGCAGTGGCGCAGTCACAGCTCACTGCAACCTCTGCCTCCCAGGCTCAAGCGATTCTCCTGCCTCAGCCTCCTCAGCAGCTGGGATTACAGGCATGTGCCACTACTGGCCGGCTAATTTCTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGGCCTCAAATGATCCACCTGCCTCGGCCTCCTAAAGTGCTGGGATTACAGGCGTGAGTCACCACGCCCAGCCATTAATTGCCAAGTCTCATTGGCTCCTAGTGTCTTAATCCACATCTGCTAAAGTTGTTTAAGGTACAGCTAAAACATCACCCAGGCTGGGCTCAGTGGCTCACACCTGTAATCCCAGTAATTTGGAAGGCCCAGGCGGGAGTATCACTTGAGCCCAGGAGTTCGAGACCAGCCTGGACAACACAGGAAGACCTTATCTCTACCAAAAATACAAAAATTAGCAGCATGTGGTGGTGCACACCTGTGGTCCCAGATACTTGGGAGGCTGAGGTGGGAGGATCACTTGAGCCCAGGAGGTCAAGGCTGCAGTGAGCTATGATCACACCACTGCACTCCAGCCTGGGCAGCAGAGCAAGGCCCTGTCTCAAAAAAATAAAATAGGGGCCAGGCACGGTGGCTCACGCCTGTAATCTCAGCACTTTGGGAGGCTGAGGCAGGTGAATCACCTGAGGTCAGGAGTTCAGGACCAGCCTGGCCAACATGGTGAAACCTCGTCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGGTACACGCCTGTAATACCAGATACTCGGGAGGCTGAGGCAGGAGAATCGCTTTAACCTGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGTGCCACTGCACTGCAGCCTGGGTGACAGAGCGAGACTCTGTCTCTAAATAAATAAATAAAATAAATAAATAAATAAATAAGGCTGGGTGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGTGTAGGGAAAAGAGAGATCAGACTGTCACTGTGTCTACGTAGAAAGGGAAGACATAAGAGACTCCATTTTGAAAAAGACCTGTACTTTAAACAATTGCTTTGCTGAGATGTTGTTAATTTGTAGCTTTGCCCCAGCCACTTTGACCCAACCACTTTGATCCAATCTGGAGCTCACAAAAACATGTGTTGTATAAAATCAAGGTTTAAAAGGGACCTAGGGCTGTGCAGGACGTGCCTTGTTAACAAAATGCTTACAGGCAGTATGCTTGGTAAAAGTCATCGCCATTCTCTAGTCTCAATAAACCAGGGGCACAATGCACTGCGGAAAGCCGCAGGGACCTCTGCCCTGGAAAGCCGGGTATTGTCCAAGGTTTCTCCCCATGTGATAGTCTGAAATATGGCCTCGTGGGATGAGAAAGACCTGACCGTCCCCCAGCCCAACACCCGTAAAGGGTCTGTGCTGAGGTGGATTAGTAAAAGAGGAAGGCCTCTTGCAGTTGAGATAGAGGAAGGCCACTGTCTCCTGCCTGCCCCTGGGAACTGAATGTCTCAGTATAAAACCTGATTGTACATTTGTTCAATTCTGAGATCGGAGAAGAACCGCCCTATGGCGGGAGGCGAGACATGTTTGCAGTAATACTGCCTTGTTATTCTTTACTCCACTGAGATGTTTGGGTGGAGAGAAACATAAATCTGGCCTACGTGCACATCCAGGCATAGTACCTTCCCTTGAACTTAATTATGACACAGATTCTTTTGCTCACGTTTTTTGCTGACCTTCTCCTTATTATCACCCTGCTCTCCTACTGCATTCCTTTTTGCTGAAATAATGAAAATAATAATCAATAAAAACTGAGGGAACTCAGAGGCCAGTGCAGGTCCTTGGTGTGCTGAGCGCCGGTCCCCTGGGCCCACTATTGTTTCTCTATACTTTGTCTCTGTGTCTTAGTTCTTTTCTCAGTCTCTCGTCCCACCCGACTAGAAATACCCACAGATATGGAGGGGCAGGCCACCCCTTCACCAAGGGGCCAAGGCAGGCGGATCATGAGGTCAGGAGTTCGAGACCAGCCTGGCCAAGAGACCAGCCTGGCCAATATGGTGAAACCCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCGGGTGCCTGTAATCTCAGCTACTGGGGAGGCTGAGGCAGGATAATTGCTTGAACCCAGGAGGTGGAGGTTGTGGCGAGCCGAGATCATGCCACTGTACTCCAGCAGCCTGGGCAATAAGGGTGAGACTTCATCTCACCAAAAAAAAAAAAAAAGCAGCTCCACTAGGAAGACTAAAATACTAAAATGATGGTGTGCTAACTTGAACAGTTAGGTTGTACAAGAAAGGACTTGACAGTTCCCTTTCTGATGTCCTCCAAGACATGGGTTCCATTTATATGCACCTTGTTCTTTCCCTCAGACCTGTAACTTCAGCCTGGAGTTGAGCAGAAACATGGCTTCCTTGTCTTCAAGTCATTCTTGGGCTTCAGAGCGAAGATGCTGGACCTTTGAACCAACAAGCAGGTTACTGGTACCTTTGCCCTGAGAATACGCTGGTGGTGCTTGTGGCTGCAGTGTTTACCCCGAGATAACTTTGCCATGAAGTATCTTCCTTTTATTATTTTTTCATCGCTCTAGTATATCGACTTTGGAAACAAAAGACATCACTCTATTTAGAGCATTCCTTTCTTAGTAGTGGTATTTCCATTGACAAAAAAATAGTAATTCTGAATTGCCGAAAATGTCAAATCGTAGAAAATGTTGTTAGCCGAAGATTCATCTGATGAATCAGATTTTTCCAAAATAGATGATTCTGATGTTAGTTCTGTTTAGAAATAACTCCAAGTACAGTTTTTATATATTATTTTCACATTGAAAATCAGTCAGATTTACTTCAGCCTCAAAGAGTGTGTTTATGTAAAAATAAATGAGCACTGGCAACCTCCGCCTCCCGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCCAGTAGCTGAAATTACAGGCGCCCGCCACCACACCTGGCTAATTTTTGTATTTTTAGTAGAGACGAGGTTTCACTATGTTGGCCAGGCTGATCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATATATAGGTGTGAGCCACGGCACCCGGCTGGCTTTTCAATGGTATATGGGAATGAGCTGCTTGGTCAGCTGGATAAATGTACTGTCAGCCTCAGGACTGCCTGCATTCACCCTGGGAAGCAGTCAAACCCCATGTGATGGGTCATTGGGTCATGAGATGCTGGAACATCACTGTATTGCTGGCTTTGGAGAAAGATAGATTTTTTTTTTTTTTTTTGAGACGCCGTTTCACTCCGTTGCCCAGGCTGGAGTGCAATGGTGTGACCTCGCCTCACTGCAACCTCCGCCTCCCGGGTCCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACCCACCACCAAGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGATCCGCCTGCCTCATCCTCCCAAAGTCCTGGAATTACAGGTGTGAGCCACCGTGCCTGGCCAAAAGGTAGATCTTGTCTCATTTTCCTGCCAGAAAGCTCCCTGAGGCTGAACATCTGAAAAAAGAACTCAGAAAGACGACCCCAGACATTTGCTGTGTACAGCGCTACCCAGAATTATTTGAAGAAAATGTTCAATTTCCTTCTTAATCTCTTCACTGACTCACTGGTCATTCAGGATCATATTGTTTAAGCAGAAAATGTTTGGGAAAAAAAAAAAAAACCACCACCACCACCACTAAAAAATAACAGCACTGGGTGGGTGTGGTGGCTCATGCTTGTAATCCCAGCACTTTGAAAGGCCAATGAGGGCGGATCACCTGAGGTCAAGAGTTCAAGACCAGCTCGGTCAACATGGCAAAATTCTGTCTCTACTAAAAATACAAAGATTAGCCAGGTGGTAGTGCGTGCCTGTTAATTCCAGCTTTTCAGGTGGCTGAGGCACAAGAATCGCTTGAACCAATGAGGTGAAGGTTGCAGTGAGCCGAGATTGTGGCCACTGCACTCCAACCTGGGCTACAGAGTTGGACTGTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAGCAAGACAGTGAAACAACTATTTACGTAGTACTTATATTGTATTAGATACTATAAGTTATCTAGAGATGATTAGATTATTTCAAGTGTATGGGAGGACTGGGGAGTTCTGCACTTTGGGAGGCTGAGGCAGGAGGATTGCTTCAGCCCAGGAGTTCAAGAGCAGCCTTGGCAACATGGCAAGATCCCAACTCTATTAAAAAAAAAATACAAATTTAAAAAGTACACAGGAGGGGCCGGGCACGGTGGCTCACGCCTATAAGCACTTTGGGAGGCCGAGGTGGGCGGATCACGAGGTCAGGAGTTCGAGACCAGACTGGCCAATATAGTGAAACCCCACCTCTACTAAAAATAAAAAAATTAGCTGGGCGTGGTGGCATATACCTGTAATCCCAGTGACTCGGGAGGCTGAGGGAGGAGAATCGCTTTAATCTGGGAGACGAAGGTTGCAGTGAGCCGAGATTGTGCCACTGCACTCAAGCCTGGGTGACAGACAAGACTCTGTCTCAAAAAAAAAAAAAAAAAGAAGTATACAGGAGGATGTGCGTAGGTTATATGCTGTGCCATTTTATATCAGGGACTTGAGCATCTGTGGATTTTGATACCCCTGGGGGTCCTGGAACCAATCCCCGTGGATACCAAGGGAAGACTCCACTCAACTCTGCCACTACAAACAGCATGTAAGCAAATGGGTGTGGCTGGGTTCCCATAAAACTGTATTTACAAACACCCAATGTCTACTTAGGTGAGCTGCACATTGACCTCTTTAGCTCGTTATTCCTTCTTGCATCTCAGATTGTTCCATCTAGTACTGGGTTTCTTCTGCTTGAGGCACACACTTTTTGGTGAGAAAATGTTGGTGGCAAATTCTTTGCTTTTGTTTGCACTTCACCATCATACTTGAAAGATTTTTTTTTTTTTAAGTCGGAGTCTCGCTCTTTTGCCCAGGCTGGAGTGCAGTGGCACAATCTAGGCTCACTACAACCTCTGCCTCCCGGGTTCAAGTGATTCTGCTTCAGCCTCCCACGCAGCTGGGATTACAGGCAAGCGCCACCATGCCTGGCTAATATTTGTATTTTTATTACAGACGGGGTTTCACCATGTTGTCTAGGCTGGTCTGGAACTCCTGACCTCAGGTGATCCGGCCACCTCGGCCTCCCAAAGTGCTGGGATCACAGGTGTGAGCCACCACGCCTGGCCAGCGACCATCATGCCCAGCCAGCCATCATTATATCTTCAAGTTTTGCTTCTGCCATACTTGCTGTCTTCTGTTTCTGTGAAATCAGTTAAATGTATGGAACACCTTCTCATTCTCCTTGCGGCTTCTTCCTCTGTCTTTAGTGTCTTCCCTGAACTTGTCTTTCTGGGTTGTATTCTGAATACATTCTTCTGAGCCAGTTTTTGGTTCCCGCATTCTCTCTTTGATGTTGCGTAATTTGCCACTAAACCAGTCCATCTACTTTTAAACTTACTAGCTCACATTTTACCTTTTTTGAGACAGGGTCTCACTATGTCGCCCAGGCTGGAGTGCAGTGGCTTGATCTTGGCTCACTGCAACCTCCACTTCCTGGCCTCAAGCGACCCTCCCATCTCAGCTTCCTGAGTAGATGGGACTACAGGCACTCGTCACCACACCTGGCTAGTTTTTGTATTTTTTGTAGACGTGGGGTCTCACCATGTTGCCCAGGCTGGCCTAGAACCCCTGGGCTCAAGCAATCTGTCTGCTTGATCCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCCAGCTGTCCCACCACGACTTATTATTTCATTAGATCTGGTTAAGTACGGTTATTTTATGTCTGGCTAACAATTCCAGCGCCTGAAGACCTCACGATCTGTCGCTATTGCTCATTGTTTCTCTTGCAATGTTGTTTCCTGGTGCGCCCGGTTATCTCTGATGATGTGCTTGCCCTCATGCTTTAAAAAGTACCTGTAGGAGGAAGGAGTCTCTCCTGAGAGGGTTTGCTCTGGGTTCTGTCAGACAGGCAGAACCATCTGCATTGGGGAACTGCCCATCCAGGACCCCTTAAGCCAAGTGAAAGGCTTAAGGTTCCATGGTCAACCCTGGGAATTTGAACCCTGGGAACTTGAACCCTGGGAATTTGAACCCTGGGAATTTGAACCCTGGGAATTTGCACCCTGGCTCAGTTGCAATTCTGGGTTTCCTCTCAGTATGATGGGGCAGCAAGTTGGGGGCCTCAAGATTTGATTTCTGTCCTCTTCGCTTTATAGGGCTGTCAAGGAAAAGTTCCTGGGTGGGCAAAGGCAACTTGGGGGTTAATTTGTATCATGGGGTCCTAGTTTTCTCTGAAAATTTGGCCTTGCAAATGTGTGTGTGTGTGTGTGTGTATGAGTGTGTGTGTGTGTATATATATATATTTATATATATATTTGAGACAGGGTCTTGCTGTGTGGCCCAGGCTGGAGTGCAATGGTGTGATCATGGCCCAAGTGAACCTCCCACCTCAGTCTCCTGAGTAGCTAGGACTACAGGCATGTGCCAACATGCCCGGCTAATTTTTGTATTATTGTAGAAACAGGGTTTCATCATGTTGCTCTGCTGGTCTGGAACTCTTGGGCTCAAGCAAAATGCCCAAAGTGCTGGGATTACAGGAGTGAGACACTGTGTCCAGCCATAGAGTCTCACTGTCCTTACAGAAAGCCAGAATTCAGTCTGCTTCTTGTTCCCTCAAAGCTTCCTCGAAGTGTTCTAGTGGAGGCGAGCAGTGAAGCTCTAGGAGGCAAGAGGCCTTCTCAGGGACCCCAGCTTTGGCCTGTATGTCTGGGTGTCGTCCTGGACTAGTCTGATCACGTCTCACAGCTGAGTTTCTCAGCTTCTCCTCCTGCAGTACAGGAAGGACAATCCCTTCCGACTGCTTCTCACAGGGAGAGGGCAGTGGTGAGCCTAGAAAATAAAGGCCCGAAGCCAGCCCCGCCCCATTTGTGCCTCTCCCTCCTCTCCTTTTCTGCTTCGCAGAGCAGACAATGCAACAGTCGCCCCCGCCCAGCTCAGCCTGAAGACCCCTTCTCCACTCCCATCCCTTTCTGGCTCAGCCACCCGGCAGTGGGCCAGGTTCGGATGGAGGGAGGGAGAGGAGCTGGAGAGAGTTTTAAGTACTCTCTGGGCTTCTCACATTGCTGGCTTCAGTGGTTGCTGGACGCAAATTGATGTGATGTCAGACACCGGCAGCAGAGAAGGAAGCTAAGGCATTTGGGTCTGTGCTGCTGTGGCACCTGGGACTCAGTGTGAAGGGATCAGGCTAGGTTTGAAATTCTCTTCCGGCTTAATAATTTGCAGGTCTGGGCTGGGAGGTAAAGCTCTGAGTACACTGCGAGTGTCAGGGGTCCGGCAGGGGGGCTTTTTCCAGCTCCTACCCACTCCCGCGTGGGGTGAAACCTTGAGAGATGAGACACCAATCCAGGGCCTCTGAAGACCCACGAGGTAGAGTGCACTTAACAGTAAATTGTGGGCTGGGTGCGGTGGCTCTTGCCTGTAATCCCAGCACTTTGAGAAGCCTAGGCGGGTGGATCACGGGGTCAAGAGATAGAGACCATCCTGACCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGTGTGTGCTGGCGGGTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGACCCCAGGTGGTGGAGGTTGCAGTGAACTGAGATCACGCCACTGCACTCCTGCACGCCAGCCTGGCGAAAGAGCAAGAGTCTGTCTCAAAAAAAAAAAAAGAAAAAAAAGAAAAAAAGAAAAAAAAAAAGAGTAAATTGTGGCCAGGTGCGGTGGCCCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGCGGATCACGGGAGTTCAAGATCAGCCTGGCCAGCATGGGGAAACCCCATCTCTACTAAAAATAAAAATTAGCTGGGCATGGTAGCGGGCGCCTGTAATACCAGCTACTCAGGATGCTGAGACAGAAGAATTGCTTGAACCTGGGAGGTAGAGGTTGCAGCGAGCAGAGATCGTGCCACTGCACTCCAGCCTGAGTGCCAGAGCCACACTCTGTCTCAAAAAACAAAAACAAAAACAAACAAAAAACCCGGTAAATTGCGGGGCCGAGTGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGCCGCCAAGGCTGGGGGATCACCTAAGGTTAAGAGTTCGAGACCAACCTGCCCAACATGATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCGTGGTGGCGTACACCTGTAGCCTCAGCTACTTGAGAGGCTGAGGCAGGAGAATCACTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCCAGTTAGTCCAGACTGGGCGACAGAGCAAAACTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGTCAATGGAAGCCCAGGGGTGGGGGTTAACGCCTTTGGCCCCAGTTTTTCAGGGGGTTGAGGTGGGGGGTTTTTGGACCCTGGGGGGTGGAAGTTGAAGGAATTGGTGATGGGGTAATTGAACTCCACCCTGGAAAAAAGGGTGAGTCCCAGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAAATTGTGGCTGAATTCTTTGGCATTGCAGGCAGAGAAGGGGTGAAAACAGGGCTCCAGCCTTGAACCGGTCAGCAGGGGTCAGGGAGGCTGAGTGAGTCACCCTATGGAATGGGCCAGGGTGGATCTGGGCTCACAGGCAAGGGCCTCAGTGCTCCTGTGAGCTCCAAGGGGCTGGGGAGGACTGCCTTCTTGTTCAGGGTACAGCTCGTGTTGTTTTCTCGGGCTTATGTCCATGTCTCAGTGTGGACACCCGCAACTGAGGTGACAGTGGAATGCCCTGACCTGATCTCTCATTCTCTCCCATTGTGGACCCAGTGCTGTCCTCACCTGGGACCTCCTGCCTCCACCCAAGTAGGCGCCCAGAGCTGAAGGGACAGTTGCCTGATGGGTTCAGGTGCCAGTGGATGGTCTTCCACCTCCCGGGTTCAAGCGATTGTCCTGCCTCAGCCTCCTGAGTAGCTGGGACCACAGGTGCCCGCCACCACGCCTGGCAAATTTTTGTATTTTTTTTGGTAGGGACATGTTTTCGTCATGTTGGTCAGGCTGGTCTTGAACTTCTGGCCTCAAGTGATCTGCCCATCTTGGCTTCCTAAAGTGCTGAGATTACACGCTTGAGCCACTGCGCCTGGCCTTACTTGGGTGTTGTTCCCTGCCCTGGAACCTACCCTGCTTAGCCTTACCACACCCCATCAGCCCCCAGCCCAACCCGACTCTCAGCCTTGCCACAGCCCCTTCTGGAGGCCACAGGAACCAGGACAAGAGGACTAAAACCACTGCCCCTGTGGCCGAAGCCTGGCCCCCAATCCTGTGCACACAGAGCCCACTGAGCCTGTGACCTGTCCTTGGCCCCCATCAAGCTCCTTTCCCTATCTAGGGTCCAGGTGAACTTGGCATACTTCCTCCCTCTGAAAACTCAAGAGGTGCCACTCCCCATTCAGGGCACAGGAAGAGCAAGAATTGAACACCGGGATGCAGCATGTGTCCCTGTTGCCACAGGTCTCACCAGAGGCCTCCTTCGTTCTACGACATCGGGCCCTGCTACCTGCAGCGCCCAGGGTGCGCGTCCTCACCTGTGCTGCGGGTGGAACACTCCTCCCTGGCCACTTTCCAGGCCACTGGTTTGCTCTCCGCAAAGGACAAATCCCAGCACCACCTGGTTATTTTGTTCCCTGCCGGCCTCACCTCCTAGAACCCGGGCCACAAGTGCAGGGACCATAGTGTCTGGTTTCGCCATCTCCCTGGCAGGACACATGCTGCACTGAAACACCCAGGGGCTGTGTGGGGAGCAGTGCAGGGGCTGAACAGCGGCCCCCAAATACATGTCTCCTGGGAGTTCCTGATGTGGCCTTATTTGGAAATAGGAGGTCTGCAGATGTCGCTGAGTTAGGACATAGAAGTCACACCATGGGGAGGCCACGAGAAGACAGGCAGAGTGAGTGGAGTGAGGTGACCACCAGCCCAAGATGCCTGGTTCCCCAGGAGCTGGAGAGGGCAGGAAGGACCCTCCCCTTGGGCCTCTGGCGGGAGTGCTGGATCTTGGACTCTGGCCTCCAGAACTGGGAGAATCAGCCTATGTTGAGAGCTGCCCAGTGTGTGGCCCTTCTGCCTGGAAGGGGTGGCTGGGCCTGGCTGTGCATCACTGTGGACTGTCCCCTCTCCCTTGGCCTCTGACATCAGTCACAAAACCCATCCCCTCTCCTTCCTAAATACTTCTGTGGACTGGCAGTGTTGCTGGAAGTCATCTTGGGTGGGGCAGGGACATGGACAGTAAGAGCGGAAGGTGACCCCACATCCTTGCACCTCAAGCTGGTCTCACAATGACAATATGCGTGGAGCGGAGGAGGACTCAGGAGGTGTCTCTGACACCAAACATGGTCATGGCTGGACATGGGGTCAGAACCAGAAGTGAGGAGGCTCCTCAGTGGCCACAGAGGAAGCACCCGGGTGACTTGGGGAGTGGGACAAGGAAGTGGGTCCTCAGGGAAGCAAATGACAAGGGGACAGGAGGGGCTGTTTGCGGATTTAATGGCAGGGCATTGAGGTTGGGAGGGGTCCCAGCTGCTGCGTCTGCTTTTCTTATAGCAGAGAGGCTGCCTACGGGTGGCACGGGGTGGCCATGGAGTGCAGAGTTGGTGGGACAGGGGACATCCAGGGGGCTCGAGATGTTGCTGGTGACAAGGAATGTCAAGTGGCACTGAGGCTGGCGGGCAAGGCCACAGGCAGATTCTCTCACGTGGGTGCTCGCCCCTTTCCTCCCCCTTGTCCCTCCCTCCCACCCTGGCCCCACTCAGGAGTGAGACCCAGTGGCCAATAAGCTCTGGGACAGACGAATGGGCGCCCTCCTCCTTCCTTCTGTTGGGCTGGAGTGAGTGGAGGAGGTGACTCAGCTCCTGGGCTCAGGCAGGGTCTGGAGGGGCCAGGATGGTCTCGAGTGCTTGGCAGCTGAGGAATGTAGCTGGGCTCGGGTAGTAGCAGCAGCGAGGGGCGGGCCAGGGTGGCCAGAGCCCGGGGCCAGGAATGTGCAGCTGAGGTCAATGGTCCCGGAGTCCTCCCGACTCGGGGTCGGGCGGCGGGAAGGAGGGTGGCCGTGGCGGGGGTGGAGGTGGGTGCCCAGGGCTCAGAGCTTGTGGGGGTTCACCCACTTGTAGGTGCCCTCATACTGGAAACCCACTCTCTTCATCAGCTCGTCTGCCTCCGTGGGGCCTCGGCTGGAGAGTGACGGGTGGAGGAGAGGCATGAGGTAGCTCCACCCTCACCCCGCCCCTGCCCGCTGGGCTCTGTCCCCAGCCCCCACCCTTTCCTCACCTGCCATAAATATAGGGGATGGGCTTGGGCTTCTCCAGCTCAATCTGGTGCAGCAGTGGGGTGAAAATACGCCAGGCCTCACGGAGCTCGTCGCTGAGGGGACATAGTATGGCTTGGGAGGCCGGTGGCACACAGGGAGGGAGGGCAAAGGCCACCCCATAGCCCACAGGTATGCAGGGGCCGGCAGCTGGGCCTCACCTGCGCACGAAGTGCATCTGGCTCCCGCAGAAGACGTCCAGGATGAGGCGCTCGTAGGCGTCAGGGAGCTTCACGTTCTGTGAGGGAGAGAGTGTCTTGCTGATGCCACTGCCTGCCACCATGTGGAGTCCCCCGGGCCCAGGCCGCCCACCCTCCACACTGCTCCTTCTCTGTAGGGCACCTTGTATCTGTTGCCGTAGGTCAGGTCCAGCTCCGACTCCTCGGGGTTGAAGAACATGCCCGGCTTCTTGGTCATCATCTTGGTGTACACGGCCTCGTTGGGCTGCACGCGGATCACCAGCTCGTTGCGCTTGCACTGCTGGTGGAAGATGTCGCCGGCCACATCATGGAACTGCAGCCTCACCTCGGCCTTGCGCTCGTTCAGGGCCTTGCCGCAGCGCAGGATGAAGGGCACCCCTACGTGGCGGAAAGGGCAGCCTCAGCACCAGCTCTCTCAGGGTGTGGACCAGTGCGTGAGTGTCTCAGTGGGAGCTCCAGTGCCCGCACACAGGGCATGCCCAGTTCTGCCTTGCTGGGCCTCGAAGGCATCACCTACCATCCCACCTCTCATTCTCCACATAGAGGACGACGGCTGCAAAAGTGGCGGTGGTGGACCCGCGGGGCACCGTGGGGTCGTCCAGGTACCCTTTGGTGGCCTCGCCCTCTCCATCGGGGTTCCCCACGTACTGGCCCAGGACCACATTGTTGGCCTGCACCTCTGAGATGCATTTCAACACCTTGACCTGAGAGAAAGCCAAGGGAGAGAATGGGCTCCTTGGGTGTTGAGTTGGGGTGCAGGGATGACTGTGGCCACAGATGTGCAGCCCTCAGGGCAGGAGGAGGCCCCTGCTTGGCTCTGCTCACCCTGCCAGAGGCCCAGCTCAGGGCCCCTCCCTGAGGACCCTCCAGGACCACCCTGGTCCATCTCGAGTCTATTCTGATGAACAAGCTGAGGCCCAGAGAGGCAATGGCCTTCCCTGGTCACATAGTGACTGTCAGGCCTGGGACATGACAACTTGGGCTTCATGACTGCCAGTCCAGGTCACCTCCGGGAGGCCACGCTGTGCTCAGAGGTGGTGACTTCTCCGGGGTTGAGGACACCTGCTCTGCATGCACACCCCAGCTCAGTGCCTCGTCACAGATGGGCCTGCGACAGGGCATGCTCCTGGGGACTGGGGTGCACCCCCTACCTTCTCATCACGGACGTCATCTGAGTTGGTGGAGGCGGGCTTCTCCATGGCCACCAGACACAGCATCTGCAGTAGGTGGTTCTGCATCACGTCCCTGGGGACGGAAGAGGCCAGAGCTCGCCTTAGCTCCCCGCCCTGTTCACCTGGTTCAAGGGCATGGGGACCCCAAACAAGGCTTCCTAGTGACAAGCTGCAAGACTCACTTCCTGATCCCCCTTGTCTTCCAGGTCCCCTTAAATCAAGGAAGGACATGGTGATGCTATCACTGAATCATAAAACCGTGGAGTGCTTGGCTGTGTAGGGGTCCAGCCCTTTCCAGCCCGGTCTGATAGCTCAGACACTTAGGTTTTGAACTGCAGGGTGAGGAGGAGCTCCCCCAAGATAGGGAAGAGTAGCCCTGCAGGGTGACTGGCTCTGCCACCCTGTGCCAGCCTCCCAGGAGAGAGGAAGAGCTCTCACCGGATGATCCCAAATTCATCGAAATAGCCCCCGCGACCCTCAGTGCCAAAGGGCTCCTTGAAGGTGAGGATAACGCAGGCGATGTTGTCCCGGTTCCAGATGGGGCCGAAGATCCTGTTGGCAAATCTGCAGGGAGGGGCAAGGTGGAGGAACTGACCTTGGGCCTCTGTGGTGCAGGGGCCACATGTGAGGGGTCACCCTTGTCTGAGTTCTGGAGGAATTCGTCCTCGGGGAGGCAGTGGGCCAGGTGAGGCTCCTGAGTACCACCCCCACCCTGGTCCCCCGGCCCAGGCTTGGCCCCACCTCAGCACCATGAGGTTCTGCACCATCTCCTTGCCCAGGTAGTGGTCGATGCGGTAGATCTGGTCCTCACGGAACAGGGAGGAGATGTGGTTGGACAGCCGGTCAGAGCTCTGCAGGTCCCTCCCGAAGGGCTTCTCCACGATGATGCGGTTCCAGCCTCTGCTGGGAGCCCGGAGCTGCGTTACCCCCTTGAACCCCTCTTCGGGGAGTGAGGATCACAGCTGCATCATTCAGACGCCCTCCCAGGGGAGTAGAGGCCAGAACCTCCTCGCCCCCGTGGCCACCTCCCGTGCCTTGTGTTCCCAGATGACCCTCTGGCTCAACACCTTCCGGGAAGCCTTCCCACCCTGGGTGCCAGGGTGGCATTGCTGGCATGCTGCCGGGGGCCCGTTCCCCTCCTGCCTCATGCTGGGTCCCCGGTGGGTCTGAGTGGCCTGAAGGCCTGTAGGGGAGCAGGGGGAGGAGGCATCCAAGCCATGGCTTCCTCATTGGTTTTGAAAATGAGAAGAGGACCTTTGCTTTACTACCCCCGCATTCAAAACCAGCCAGAGGACAAGAGCCTCTGGCTGGGTTGGGAAACCACCCAGCGCGGCCATGCTGCATTCGCAGAGCAAGGCTGCCACCCTGCGGCCTGGCCGGGCCTTTGGGGAAGCAGAGCGGAAAGGCGGTGTTTCGTGGAGCAACGCTGCCACCTTGTGGTCCCGCTGGGGATGGCCCCGGCACCATGGATGCTGCCCAGATCCCCGGCCCCGGACACGCTCATAGAGTGGTGGGAGCACTGCCTGGGCCAGCCTGGCAGGCGGGAAGGGAGGGCAACGGCAAGCCTTACATCTGGCTCATGCAGGACTCGTGAATGTTCTTGGTGACGGCCTCGTAGACGGTCGGGGGCAAGGCCAGGTAGAAGAGGCGGTTGGCCTGTGACCCCAGGTGGAGGGCATCCATGTGGCTGTTGAGGCGCTGGTAGGAGGCTGCATCATCGTACTGGCCAGCCACATAGGAGTTGCGGGCAAAGAAGTCCTCCAGCTTGAGCTTCTCCTCTGGGGTGGCCTGGGAGACACGGACAGACAGACACACAGACAGATGTCAGCCCCTCTCTTTGAGTCCGTGTGTGTTCTGCCCCAGGGGAGTGGAGGGTCTTCCCTGGAGGTCCAGGGAGGGTGCCCTCAGAAGTCAGTGTCCCCGTCCCACACTGGGTTCAGCCCCATCTTAGCAGCTCTGCACATCCAGAGGGAGGGAGGCCAAAGCAGGCAGCACAGACACTGCCCCAGGCTGGGACCCCTGTGCCTTAGGACAGAGGCCAGATTTCAGGATATTTTGACCTGGGAGAAATACACTGGAGAAAGCTCTCTCTCCAAAATCATGACACCCAACTATGATTGGCGGAGAAAACGCAGCAGAGCACAGCAGGAGGGACCTGTGGGTCCTGGTCACGGGGGCTGGTAATGGGGGTCTCAAGAAGTACGAGAGCAGGCGGGGCGGGGCAGGAGAGGAGGAGAGCATCCCGGGATGGGATGGGGGGAGGTCCCCGAAGCTGGCCATGCTGGGGGCTGGTAGAGAGGGCAGAACCAGGCTGGGGGAGGCCCTGACACCACCCACCTTGAAGAAGGGCTCACTCTGTTTGCGGATGTCAGCCACTGTGAGGCGGGAACGGGCATAGCCCATGATGAAGGTGTTTTCGGGCAGAAGGCCATCCCGGAACAGCCACCTGAGGGCAGGGCACAGCTGTAACCAGTGCGGGCAGGGCAGGACCAGGCCTGTCCCTGGCGGGAGGTCACAGGGGCAGTGGTGGGACACACTTACCAGATGGTGGGGTAGATCTTCTTCTTGGCCAGGTCACCCTGTGGCAGAGGGAACAGGTGTGTGGTTAGAAGTGGCTGGGGACACGACCTACATACATCATCCTCCACCCTTGGTGATCTGGGCACTACTCAGGATCACTACTGGGCCACAAGCATGTCTGTCTTGCCTGATACACAGACAGAAGAGCCCCGAGATTCAAGCCTGTGCCAGCCCTCCATCCCTGACCCAGAAGACATAAACGCATCTCCTGCGTTCCAGGAGAAAACCTGAAAATTCAATTCAGTAGAACCAAGGGACGACAGAAGTATCATGTAGCCACATTTGTGAGACGTGACCTCAAGTGCTTACATTAGCAAAAAAGAGTGGTTCAAAAATAAGAAGCTAAGCATTCCACTTAAGAAGTGAGAAAAAGGGCCAGATGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAAGTCAGGAGTTCAAGACCAGCCAATGTGGCAAAACCCCATCTCTATTAAAAATACAAAAATTAGCCAGGTGTGGTGGCAGGCACCTGTAATCCCAGTTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGTGACAAGAGTGAGACTTCATCTCCAAAAAAAAAGTGAGAAAAAGATAGAACTGCAAGCCAAATTACCAGGAAGAAAATAGGAAATAATGACAAGTAAAATTAATAAAATTAAAAGCTAACCCAAAGAAAAGATGAACAAAGCTGGAAATTGGTGTTTGAAAAAGACGAATAGAAACAGACAACCCCCTGGCATGGTTGCTTTTTTAAAAGGGCATAGGCCGGGCACAGTGGCTTACTCCTGTAATCCCAGCACTTTGAGAGGCCGAGGCAGGCGGATCAAGAGGTCAGGAGATCGAGACCATCCTGGCTGACACGGTGAAACCCCGCCTCTACCAAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGTGTCTGTAGTCCCAGCTACTCGGAAGGCTGAGGTAGGAGAATCACTTGAACCCGGGAGATGGAGGTTGCAGTGAGCCAAGATTGCACCATTGCACCCCAGCCTGGGCGACAAGAGTGAAACTCCGTCTCAAAAAACAAAAAACAAAAAAACAACTAAACAGCTGGACGCGGTGGCTCAAGCCTATAATCCCAGCACTTTGGAAGGCCGAGGCGGATGGATTACCTGAGTTCGGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCGTTTCTACTAAAAATACAAAATTGGCTGGGCATGGTGGCCCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATCAAGCCACTGCACTCCAGCCTGGGCTACAAAAGCGAAACTCCGTCTCAAACAAACAAAAAACAGAGCAATGGACCTGAGAGGGGACAGTGGCCACAAATCTTCCTACAGAAACAACCCCCTGGCATAGAGAGTGTTGCCAGTGGGTTCTACCAAAATGCAAGCACAAGAGAATTCCAGCCTGAAGCAAACTCTTCCTGCAGAAGGAAGAAAGGGGAACACTTCCCAAGCCACCTTATGGGGCCTACAGAGCCTTGGTACGGAAACCTGATGAGAAAGGCACATGGGAAAACCCATACGCCTCATTCACACACACATGCAGATGCAGAAATCCCACACGAAATATCAGCAGGCCGGGCACAACTCACACACCCCAGCACTTTGGGAGACCAAGGTGGGAGGAAGATTGCTTGAAATCAGGAGGTTTTTTTTTTTTTTTTTTTTTAAAGACATGGTCTCACTCTGTCACCTAGGCTGGAGTGCAGTGGCACGATCTCAGTTCACTGCAACCTCTGCCTCTGGGCTCAAGCAATCCTCCCACCTCAGCCTCTGGAGTAGCTGGGACTACAGGTACATGCCAACACACCCAGCTAATTTTTTGTATTTTTTGTAGAGACAGGGTTTCCCCATGTTGTCCAGGCTGCTCTTGAACTCCTGGGCTCAAGTGATCTGCCCGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACGACGACCCACTGACCCTGTCTCTTTAAAAAAAAAGAAGAAAACATCAGCAAACCAAATCCTGCAATGTTAAAAACGGTAGGTTTTTTATTTACAGAATAAATACATCTGTAAATAAAAAGTTGGTTTGATATTAACAGAAAAAAATCATTGAAATTCACCATATTAAGAGATTAAAAAAAACCTCTAAGAACTCTCTGCCTACTGCTATGTTTTGATTTTTTAAATCTAGCAATCTTGCTCAACCCAACAGATGTAGATCAAGTGTTTCAGAAATCACCTATTCACGCTAAAACTCTTAGCAGAGGGGGAATGCAAGGGAACTTCACTAACTTGATAAAGGGCATCTATAAAACACCATTCTGGCTGGGCGTGGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGGAATCGCTTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATGGTGAAACCCCATTTCTACTAAAAATACAAAACTTAGCTGAACATGGTGGTGTATGCCTGTAATCCCAGCTATTCAGGAGACTGAGGCAGGAGAATCGCTTGAACCAGGAGGGAGAGACTGGAGTGAGCCGAGATCACACCACTGTAGCCTGAGCAACAGAGCTAGACGTCGTCTCAAAAAAAAAAACCACCACCAAAAAACAAAGAACAAAAAAACCCCCAAACCATCATCCTTACAAATACTACAAATATCACACTTATAGGAAATGCTGAAATATTAATCATCCCTTTAAGCAATCAGGAACAAGAAAAGTGCAGTAAGGCAAGAAAATGAAAATGGCTGAAGTTGAGCTGTGAGGTGGTGCGGGTGCTCCCTCTGATCAAGTGAAACACACACCTATCCTGTGACCTGGCCGTTCCACCCCAAGAGAAATGAAAGTGCAAGTCCACACAAAGACCTGCACACGAATGGTCACAGCAGCCTTGGTTCATAGCGGCCCCAAACTGGAAACAAGTCAAACACTTGAATTAACATGTGACCCGGCAAACTGTGCATTCATACAACGGATACTACTCAGCAACACTGGGAAATCTCAGCATCAGAGATGCAACCCATTGTGACAGAAATCAGATCAGCGGTTGCCTGGAGCAGGGCAGATTGGCTGGGAATGGGTACCCAGGAACTTTCTGGGGAGATGACGATGGCCTTTATTGTGATGAGGGTGTACAGCCAATTGTACAGTTAAGATTTGCGCACCAACAAAAGACGGCGAGAAAACCAACAATGGAGCGGAGGGTGGGGAGTGGGTCAGAAATGACCCTTTGAGGGCCAGTGACTGTGAGAGCCCCAGGTGAGGGGGCTTCCTGGGTGCAGAGCATATTCTGTTTCTTGATCTAGGAGCAGTTTCTGTGGCTGTGCTGAGTTTGTGAAAAGTCATCAACAAGCTAGACCTGCACCATCTGTGTTCTTCATGACATGTATTTCTTTTTTTTTTTGAGACGGAGTCTCACTCTGTCGGCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGAAATCTCCACCTCCTGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTCCAGGCACCTGCCACCACGCCCGGCTAATTTTTGTATTTTTACTAGAGATGGGGTTTCGCCATGTTGGCCAGGCTGGCCTCAAACTCCTGACCTTGTGATCCACCTGCCGTGGCCTCCCAAAGTGCTGGCATTACAGGCGTGAGCCACCGTGGCCGGCCCATGACATATATTTCAATAAAACATGGTAAAAACTAATAGTCTGGGCGTGGTGGCTCATGCCTGTAACCCCAGCACTTTGGGAGGCTGAGGAGGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCAAACATGGTGAAACCCCGTCTCTACTAAAAATACAGGCCAGGCGCGGTGGCTCATGCCTGTAATCCTAGCACTTTGGGAGGTTGAGGTGGGTGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCCAACATGGTGAGACCCCGTTTCTACTAAAATACAAAAAATTAGCTGGATGTGGTGGTGCGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGGGAATTGCTTGAACCTCGCAGGTGGAGATTGCAGTGAGCCAAGATTGCGCCACTGTACTCCAGCCTGGTGAAAGAGCAAGACTCCATCTCAAAAAAAAGAAAAAACAAAAATTAGCTGGATGTGGTGGCAGGCACCTGGAATCCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCACTTGAATCCAGGAGACGGAGGTTGCAGTGAGCCAAGATTGCGCCACCAAACTCCAGCCTGGGTGACAGCAAGACTCCATGTCACGAAAAAAAAAAAAAAAAAACACATGCTCTTCGAAGCAATCAGGCACTGCAACCATGGATGGGTCCATTTCTACAAACCATTACAGAATGGGGGCCATGCCACCAGGTCAAACGCCCTCACCCAGAGCCAGGCAGTTAGGAAGCCACCACAGGGCTTCCAGGAGGGCAACTGCTATGGTGTGGGTGCCTTGTGTGCTTGCGCTCTTCAGGGGCCCTGCTCCACTTCTGATGCTCCTCAGGTGAGCTCGGTGCCAGGACTACAGCGACACGGACCTCTCACTGCTGCACCCTTCAAAGTCCAGCAGACAACCCCTCGCCCTGGGGTAGGAGCTCCTCTACTCAGCTCCTCACAGCACCTGCCATCAGTGGGGCTGGGGAGGGGAACGTTGCCCATGTCACATCAACTCACGGCTGGCCCCACACAGAAAGGGTCAGCTTACTGGGAACTAACTGGACCCTGAAATAAACGGAAAGAACCACAGCCTGCAAATACCTCTAAGCCTCACTCAAAGCAGGTCATGTTTGCTCATGACCAAATTCCGGAAGACTGGGAGGCATCGGGACAGACTCCAGAAAGGTACTGGTGGCAGGGGACTGGTCTGCTGAGTCACAGCAACCCCACGCTCCAGGCCGTTTGTTCCTGCTGCTCCCTTTGGGATTAACTAGAGATTGAACCGGCTCACCTTGTTTCAGCTCAGGCCCTGCAACCGACCGACCGTCTGCTGGGCTCCAAGTCACCTGAGAGGCGAGGTGAGGTAAGACGGACAGTAGGGGTGGGGTAAGGCAGGCCGCTCTCTCCCATGGTGACAGGGAGCTAAGCTGAGCTGAGCCGGACTGAGCTGCTCTCCTCAGGACTCAGGGGTAGCCCTGCCCTCCCAGCTGGCTGCGATGCTCTAGTGGGACTTTCTGGAGGAACAAACATGGGAAGACATCGGGAAACCTGACAGCTTGGTCAAGAGTCTACTCGTGCCATGTGGGTGGGGGCAATTCAAGGGGTCCTTAGTGAAAAGCAGTTGGGAGCTTCTCATCTTCCCCACGCCCTCCTCTTTCCTGAGGACCACAGCAGGGGAGGGATCTGCCCAAGGACACAAGGTGACTTAGTAGAAGCAGGGCATGATGAAGATCTCTAGCCACCAACATCTGGCTAGGCCAGGGCAAAGGGACCTGGGCCATGACCTGTCTGTTTTATAAACCGAAATCGCTGCAGCCCTTGTCTTGGCATGAGGGTTTCATGGTAGAGCACGATGCCTGCTGGAGGGCCAGCGGGGGTGGGCCATCCCATCCCCAAGGGTCACTGGGGTTAAGGACCTGCCCAGACTCCCTGGGTGGAGCCCTGGGCCTTGGCAGGGGCCCAGCTGGTTCCTGGCCACTGGGGCCAGAGAACCTGGGGGCTGCTGAGTTAGTTCAGCTGAAAAACCAACAAAAGGGCCTCCCTGCTATACCCGGGGGCTCATGAGTCACCGGGGCTTTGGGGGAGTGCCAACATCATCATGACACAGCAAGAATCTTATCAGACCAATGGGGAAGTCAGCCCAGAAATGTTCTGAGGAAAGGGGAGGCGGGGGCCGCTGGGTCATCCCTCCCACAGGCCCATCATTGGGATGCGTCCTGAACGCCCATCAAGCCCATGGCCCTTGTGATCCAGGTGGGGAAACTAAGGCCCAGAGAAGTGAGGACCCCGCAGACTATCAATCCCAGTCTCTTCCCCTCACTCCCTGTGAAGCTCTCCAGCATCATCGAGGTCCCATCAGGTGGGGAAAGATGCTGTTCCAGGCGCACACTAGTCTACAAGGCCAGAGCTTTCTGGAAGGGGGCAGTAAGTACCTCGGCTCCCTTTCTGGTAGGGGTGGGAGTCCTGAGAAGGCAGGAAGTGGCCCACTTGGTAACTCTGAGGTGCCATCAGGGCCCCCAGGAAGGAAGCTGGGTGTGTGGGCAAGTGTGAGGTAAGCTGGCCAGGGAGGAGGAAGGGACAGAGGAAGGCCACGTGGGTCCAGCCTGCCCCAGGGTGTCCTGCTTGCCCAGGCTGTGGGTCTGCCAGCCACTTGCCTGCTTTCAGTTTCTAGGTCATGCTGAGCTTGTTCCCAACTCGGGGCTCCGGGCATTTGCTCTTCCTTCTGTGTTTGTGCTCTCCCGGCCCTCTTTGTGGGATCTATGCGCTTTGGGGGACTGGGGACACAGGGCCCATGTGTATCTTCTGAAACACACGTCAGCCTGAACTCTTGCTGGTCTGCTTACTTGCCGTGGTTCCCTGGCCGCAATAGCACATAGGTCCCATGAGGAGGGCAGGGACTGGCTGCTGTGGCCCAGAAGTACCCAATCGCTGTCTGCTCAATGAACGGAGAATGGGCTGCTTTCCTGGAACAGCAGATTCTAGGATCACGTGCCCTCAAGTGCCACCCTGCCTACCTCCCGCACCGAGTGAGGCATCAGGCGTGGAAGAAGCCTGGGAGCCGGAGCTGTTCCAGGTGCTGCCTGAGCACGCCACCTCCCATCTCCCCCACAGCAGGAAGAGGAAAAAACAAACCACGAGGCTCTTCAGAGAGAGGACCCCTTGTCCCCTACCCACAGTGCTGGAGCTGGCACTTCCTATTTCTGCTTTGAAAGCCTCAGGTTGTCACTCTCAGAACAGAGGAGAGCAAAGGGGAACCCTACTGATTTCAACAAAACAAAGTTGCCCAACCCGAAGCTGGCCACAGGCGGAGGCAGTGAAATGACAAAATCAGCTTGGAAAAACCTAAGGACCCTGGGCCCTCGTTTCAAAAGCTGTCATTTGCTACACGAAATGCTGAGGTTCAGGAAAAGGAAGACACTTGCTCCAACTCACACAGCAAGCTTGGATGCTCTCAATGAGGTGTCTAATAAGAGCTGAAGGCCAGGAGTCATAAGCTATCATTGTGGCCTGGGTTGCTCTGTTGCAGTGCCTTGTGACAGCATGGGGTGGGGATGGAGAAAGCAACCTCAGTTACCTTCTCCTCCAGTCTGCTTCTTGGACTAAGGGTTTAACGGTCAGAGTCCTGGCTGTTAAGGTTTGTGGCTGATGCAGGTATGGCTCTTTTTATTTTTATTTTTTTGAGATGGAGTCTCACTCACTCCGTCACCCAGGATGGAGTGCAATGGTGCCATCTCAGCTCACTGCAACCTCCACCTCCTGGGTTCAAGCGATTTTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGATGGGTGCCACTACACCTAACTAATTTTTGTTATTTTTTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCACCCAGGCTGGAATGCAGTGTTGCGATTTCTGCTCACTGCAAGCTCCGCCTCCCGAGTTCACGCCTTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGACTACAGGTGCCCACACCAGGCCTGGCTAATTTTTTATTTAGTAGAGACGGGATTTCACCATGTTAGCCAGGATGGTCTCAATCTCCTGACCTCATGATCCACCCGCTTCAGCCTCCCAAAGTGCTGGGATTACAGACGTGAGCCACTGCGCCCGGCCTTTTTGTTTTGTTTTGTTTTGAGACAGAGTCTCGCTCTGTCGCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAACCTCTGACTCCCTGATTCAAGCGATTCTACTGCCTCAGCCTACTGAGTAGCTGGGACTACAGGCACGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCTTGACCTTGTGATCTGCCCACCTTGGCCCCCCAAAGTGCTGGGATTACAGATGTGAGCCACTGCGCCCAGCCAATTTTTGTATTTTAAGTAGAGACTGGGTTTCGCCATGTTGGCCAGGCTGGTGTCAAACTCCTGACCTCAGGCGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGAATACAGGCGTGAGCCACCGTGCCCGGCCAGGTATGGCTCTTCTGAGGGGACCAGGCTGGGGCTGGGGCTGAGGCCAAGCCCAATCTACTGTGGGCTCCACCTGGTACCTCTCCTGGGTCTCAGGCTTATGGGGAGTCAGAGGACAATGGCCCCTCCTTACTCTGCCACTGGCAGAGCCCTTCTCCCTCGGCTGCCTCCTCATTCCCTTTTGGCTCTCCTTTTCTAAGTTCTGATCAGAAGTACAAAGGTGTCAAGGAGTAGGTTTGACAAAGTGTGACAGCGCGTTGTTCTATGTGAACAAAGAACCACTGAGCTCAGCCAGCACTGAGGGGCGCACGATGTGGAAGAACTAACTAGTTTTGATAGAGCTCCTGCTCAAGGTTACAAGGTAAGTTAATGGCAAAGATGGTCATACAGTAATGAGCAGAGAGATTCTTGAGTGCATCCAGGGTTAAAAGTGAAAACTGAGACCACGTGTAATTTGAGATGAAGCCCTTGTTCCACCAGCCCCTGCACAGTCTCACTGCCCCATGGGACACAGGGGAGGGTGCTCTAGTCTCGGGCGATGGCTGTCCTAGGACCACCCCTCCCTCCCCTCCCATGGAAATCCTCATGCTATACTATTCTTTTGTCTTTCCTATGAGTGCAGAATGGCGGTTCTACAAGCTGGACAATTGGGGCCAGGTGGGGTGAGGCAGAGCTCCTTGGTAGGCTTTCGAAATTGAGGCAAAGAGACAGTGTTCAAAGAAAAGCTAAGTGTTTGTATCGACGGAACTTGAAGTGTTAGTGAAGAGGCAGAGATCAGGCCTCAGATCCTGCCTTTGGAACTCATTTGTTAAGGCATGAACAGGTCTGAAAAAACTACAGAATGAATAGCATTCCCTGTTTTCCCCAAGAAGTCCATCTAGACAGTCCCTAAAGAGCCTGCAACTCCAGGATTAAGGGCTACATTCAGCGGCTAGGCACAGGGTTCAGAAACGTCCTGCAGCCGGGCGTGGTGGCTCATGCCTGTCATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGTCAACATGGTGAAACTCCGTCTCTACTAACAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCTCAGCTACTTGGGGGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCCGAGGTTGCAGTGAGCCAAGATGGCGCCACTGCACCCCATCCTGGGCGACCAGAGCAAAACTCCGTCTCCAAAAAAAAAAAAAAAAAAAAGTCTTGCAAGTGCATATGCACACCAGGTAGAGCCGGGATGATCCTGGCGCACTAGCAGGAGCGGGAGGAGGAGCTCAACTTAGCAGAGCCTGTGGGGCCCTGCAACAATTAGTTGGAAAAGCTGAGGCATGGAGCAGGCACTTCCTGGCTTTTAAGATTGGGGCCTGGGAGATACTCACCGATGCACCCATGATGATGAATATGTGTGTATCCGACTGATGGAAGGCATCGCCCTGGAAAAGCTCTTCCCGCAGGATCCCGCACACCTGGGTCCGGCTCAGGGCCACCTGCTCTGCCATGACGCTGTCTGGTGGAAGAAAGGCTCGTTAACAAGGCAGAAGAACAGGAGAGCATTGAGAAGTTAGCCCCTTTCTTGAGAGTTCCTCTGGGGTCTCACACCAGGGTGACACCTGATTGCCCAAATCACTCCTTGTGAACGGCTGGGCATTGGGGAGTGGTTGATGGGTTAGAAACTGCTGGCTCTGGGCTCCAGCCACTCTCTCCTAGGCAGCCTTCATTCAGGGTGTACATTACAAAATTACTCAAAGCATTGGTGTATTCCGACTACAGCATCAATTCTGGACAACCGAGTAAAATCCTTGTGGGGCATGGAACTGCGTGCCCAGGACCATCTCATTCCCGACTGTAGCGGGAACTCCACAATGACCTGGAGCATGGGAGATGGTGCAGATGCTGGAGTTCAGCTCCAGCCCTCCCCTTGCCAAGCTGGGTGACCCCGGTCGGCAAGTCCCCTTCGCTCTCGGGGTCTCAGGGGCTCAAGAGAGGAGGTGCGGGGTATAAAGGGATTGGTTAAGACCCTCTCGATTCTGCTCGGTTCTCAAGCACAACAAACAGCGTGTATTTTACCGCCGCGCGGCGCAGCGCGGGACAGTACGCTCCTCCGCCTGCGCGGCGCCCGCCCGGCCGGTTACCTGCGCTTCGTCGTCGTCGCCCTCCGCGCTCGCAGCCCCGAAGTGTACGACCGTTTCCGGGGGCTGAGCCCCGCCGGCCCATTTAATCGGCGGGGGCGGGGGCGGGCGCCTGGGCTGAGCGGACCCGCCTCAGGCGAGGCGTGCGGGGCGGGGCCTCGGCCACCACCCCTCGTGCGGGCGGGGCGGGGCGAGGGCAGGTGCGCGCGCATCCCAGGCCAGCCCCTGCCTCTCGGGCACCTGCGCTGGAGGCCGGCCCGCCGGCTGCCTGCCATACCCGCTGCCGCTGCTCTGCATCCCCAATTCCGGCGGGCACGGGTGCAGCTCCGCGTAGTGCTCCCGCATCCCCATCGCCGGCCCGGCCCCGCCCCTACTGTCCGGTTTCCCCGCCTGCCCCGGCGGCCTCGCGCGCTCGCGGAGGGCTCCACTTCCGCCGGCAGCGTGGCCACGCCTCCTCTGGGCTGTCCCTCGGCTCCTGGGCGGGGCCTTCTGCCGCGCCACTCCCCCGGGAACCCTCGCCTGTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCGAGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGACTTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCACAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCCGCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGGTGTGGTCCTTTTCCCCAAAGACAGGGTTCCATCCGTGGGCGTTCCGCCGCCTCCGAAACTTCCCCCGGACGTTCAGGCTCCCCCCTCTTTTTTGGGCCCCAGCCCGTTCCTGCTCCGCGCTTCTGGAGCACTGGCCAAGGCGGGCCGATTCAGGACCCAGGTTACTTGGGCGGCGAGCTGGACTGTTTCTACTCCTCCCTCCTCCTCCACTGCGGGGTCTGACCCTACTCCTTGTGTGAGGACTCCTCTAGTTCAGAGACATATTCTGTTCACCAAACTTGACTGCGCTCTATCGAGGTCGTTAAATTCTTCGGAAATGCCTCACATATAGTTTGGCAGCTAGGTATCTGATTTCATATGCCTGTTTGCTCGTTTTGCAAGACAACATCTGCCTATCGTCATACTGTTTCTGTGATCTGAGAATGAATGGGCTCTCCTGGCACATTAGAGGAATAGCACGGAGGTCACTATAGCTGGAGCAGAGTGAGGGAGGGGAGCACAGTTGGAGAGGAAGGGATGGGGAACCCGATGGTACAGGGCCTTGTAGGCCGTTGTAGGGAGATTGGCTTTTACGCAGAAGGCAACGGGGATCCATGGTAGGGTTCTCAGTAGAGGAGGAATGTGATCGGAATTACGTCTTACACTGATCGTTCTAGCAGTGGTGGGGAGACCAGACCATAGTGGGCAAGGGTAAAAGAAGGGTGGCGTGGTAAGAGGTAATTGCAGTAATCCAGCTCAGAGACAGTGCTTTGAGCATTTTATTATTGGAAATTTCAAAAATGTACGAAAGTAGAAAAATGAGTATAGTAAATCTTTTTGGTTTGTTTGTTTTTGAGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTCTAACCTCCTCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCTTCCCAGGTAGCTGGGACTGCAGGCGTGCGCCACTACGCCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACTATATGTTGGCCAGTCTGGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGACTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCGCTCGGCCAGTATAGTAAATCTTACATTCACAGCTACCAACTTCAACAATTGAAGGTATCAACTTCTGACTAATTTTGTTTATATCCCTCGCAATCCTCTCCCATTATTATTTTATTTCTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTTTTTGAGACAGAATCTCGCTCTGTCACCAGGCTGGAGTGCAGTGGTGTGATCTCCACCCACTGCAACTTCCGCCTCCTGGATTCAAGCGATTCTTCTGCCTCAGCCGCTGGGACTACAGTTGTGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCATCATGTTGGCCAGGATGGTCTTGATCTCTTGACCTTGTCATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCGGCCTCTTTTTTTGAGACCCAGTCTCACTCTGTCGCCCAGAATGGAGTGCAGTGGCACGATCTTGGCTCACCGCAACCTCCATCTCCCAGGTTTGAGCGATTCTCCTGCCTCAGCTTCCCAAATAGCTGGGATTACAGGCATGCACCATCACTCCCAGCTATTTTTTTTTTTTTTGTATTTTTGGTAAATACAGGGTTTTGTCATGTTGGCCAGGCTGGTCTTGAATTC PRIMER_OPT_SIZE=27 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=30 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.021135 PRIMER_LEFT_0_PENALTY=0.016014 PRIMER_RIGHT_0_PENALTY=0.005121 PRIMER_LEFT_0_SEQUENCE=CTCCTCCACTCTCAGTACACATGGTTT PRIMER_RIGHT_0_SEQUENCE=GTTTACTATGGCACACACATTCCCATT PRIMER_LEFT_0=3159,27 PRIMER_RIGHT_0=3425,27 PRIMER_LEFT_0_TM=64.984 PRIMER_RIGHT_0_TM=65.005 PRIMER_LEFT_0_GC_PERCENT=48.148 PRIMER_RIGHT_0_GC_PERCENT=40.741 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=267 PRIMER_PAIR_1_PENALTY=0.028467 PRIMER_LEFT_1_PENALTY=0.012719 PRIMER_RIGHT_1_PENALTY=0.015747 PRIMER_LEFT_1_SEQUENCE=CAAGGATTAATAACAAAGGTGGCATCA PRIMER_RIGHT_1_SEQUENCE=AAACCCATCATGAGACTGCAAAAACTA PRIMER_LEFT_1=4171,27 PRIMER_RIGHT_1=4344,27 PRIMER_LEFT_1_TM=65.013 PRIMER_RIGHT_1_TM=64.984 PRIMER_LEFT_1_GC_PERCENT=37.037 PRIMER_RIGHT_1_GC_PERCENT=37.037 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=5.7000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=174 PRIMER_PAIR_2_PENALTY=0.031051 PRIMER_LEFT_2_PENALTY=0.016014 PRIMER_RIGHT_2_PENALTY=0.015037 PRIMER_LEFT_2_SEQUENCE=CTCCTCCACTCTCAGTACACATGGTTT PRIMER_RIGHT_2_SEQUENCE=GACTTAGGATGGCATTCCCAATACTTC PRIMER_LEFT_2=3159,27 PRIMER_RIGHT_2=3348,27 PRIMER_LEFT_2_TM=64.984 PRIMER_RIGHT_2_TM=64.985 PRIMER_LEFT_2_GC_PERCENT=48.148 PRIMER_RIGHT_2_GC_PERCENT=44.444 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=190 PRIMER_PAIR_3_PENALTY=0.067964 PRIMER_LEFT_3_PENALTY=0.057575 PRIMER_RIGHT_3_PENALTY=0.010389 PRIMER_LEFT_3_SEQUENCE=TCAGTAAAATTCACCTGGAACTAGCCA PRIMER_RIGHT_3_SEQUENCE=AAGCCACCAAATTCTTCATGTCAGATA PRIMER_LEFT_3=1752,27 PRIMER_RIGHT_3=1928,27 PRIMER_LEFT_3_TM=65.058 PRIMER_RIGHT_3_TM=64.990 PRIMER_LEFT_3_GC_PERCENT=40.741 PRIMER_RIGHT_3_GC_PERCENT=37.037 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=5.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=177 PRIMER_PAIR_4_PENALTY=0.071844 PRIMER_LEFT_4_PENALTY=0.056807 PRIMER_RIGHT_4_PENALTY=0.015037 PRIMER_LEFT_4_SEQUENCE=ATGAGGTACATTGAGTATCCACAGGGA PRIMER_RIGHT_4_SEQUENCE=GACTTAGGATGGCATTCCCAATACTTC PRIMER_LEFT_4=3056,27 PRIMER_RIGHT_4=3348,27 PRIMER_LEFT_4_TM=64.943 PRIMER_RIGHT_4_TM=64.985 PRIMER_LEFT_4_GC_PERCENT=44.444 PRIMER_RIGHT_4_GC_PERCENT=44.444 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=293 = SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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CAGCGAGGGGCGGGCCAGGGTGGCCAGAGCCCGGGGCCAGGAATGTGCAGCTGAGGTCAATGGTCCCGGAGTCCTCCCGACTCGGGGTCGGGCGGCGGGAAGGAGGGTGGCCGTGGCGGGGGTGGAGGTGGGTGCCCAGGGCTCAGAGCTTGTGGGGGTTCACCCACTTGTAGGTGCCCTCATACTGGAAACCCACTCTCTTCATCAGCTCGTCTGCCTCCGTGGGGCCTCGGCTGGAGAGTGACGGGTGGAGGAGAGGCATGAGGTAGCTCCACCCTCACCCCGCCCCTGCCCGCTGGGCTCTGTCCCCAGCCCCCACCCTTTCCTCACCTGCCATAAATATAGGGGATGGGCTTGGGCTTCTCCAGCTCAATCTGGTGCAGCAGTGGGGTGAAAATACGCCAGGCCTCACGGAGCTCGTCGCTGAGGGGACATAGTATGGCTTGGGAGGCCGGTGGCACACAGGGAGGGAGGGCAAAGGCCACCCCATAGCCCACAGGTATGCAGGGGCCGGCAGCTGGGCCTCACCTGCGCACGAAGTGCATCTGGCTCCCGCAGAAGACGTCCAGGATGAGGCGCTCGTAGGCGTCAGGGAGCTTCACGTTCTGTGAGGGAGAGAGTGTCTTGCTGATGCCACTGCCTGCCACCATGTGGAGTCCCCCGGGCCCAGGCCGCCCACCCTCCACACTGCTCCTTCTCTGTAGGGCACCTTGTATCTGTTGCCGTAGGTCAGGTCCAGCTCCGACTCCTCGGGGTTGAAGAACATGCCCGGCTTCTTGGTCATCATCTTGGTGTACACGGCCTCGTTGGGCTGCACGCGGATCACCAGCTCGTTGCGCTTGCACTGCTGGTGGAAGATGTCGCCGGCCACATCATGGAACTGCAGCCTCACCTCGGCCTTGCGCTCGTTCAGGGCCTTGCCGCAGCGCAGGATGAAGGGCACCCCTACGTGGCGGAAAGGGCAGCCTCAGCACCAGCTCTCTCAGGGTGTGGACCAGTGCGTGAGTGTCTCAGTGGGAGCTCCAGTGCCCGCACACAGGGCATGCCCAGTTCTGCCTTGCTGGGCCTCGAAGGCATCACCTACCATCCCACCTCTCATTCTCCACATAGAGGACGACGGCTGCAAAAGTGGCGGTGGTGGACCCGCGGGGCACCGTGGGGTCGTCCAGGTACCCTTTGGTGGCCTCGCCCTCTCCATCGGGGTTCCCCACGTACTGGCCCAGGACCACATTGTTGGCCTGCACCTCTGAGATGCATTTCAACACCTTGACCTGAGAGAAAGCCAAGGGAGAGAATGGGCTCCTTGGGTGTTGAGTTGGGGTGCAGGGATGACTGTGGCCACAGATGTGCAGCCCTCAGGGCAGGAGGAGGCCCCTGCTTGGCTCTGCTCACCCTGCCAGAGGCCCAGCTCAGGGCCCCTCCCTGAGGACCCTCCAGGACCACCCTGGTCCATCTCGAGTCTATTCTGATGAACAAGCTGAGGCCCAGAGAGGCAATGGCCTTCCCTGGTCACATAGTGACTGTCAGGCCTGGGACATGACAACTTGGGCTTCATGACTGCCAGTCCAGGTCACCTCCGGGAGGCCACGCTGTGCTCAGAGGTGGTGACTTCTCCGGGGTTGAGGACACCTGCTCTGCATGCACACCCCAGCTCAGTGCCTCGTCACAGATGGGCCTGCGACAGGGCATGCTCCTGGGGACTGGGGTGCACCCCCTACCTTCTCATCACGGACGTCATCTGAGTTGGTGGAGGCGGGCTTCTCCATGGCCACCAGACACAGCATCTGCAGTAGGTGGTTCTGCATCACGTCCCTGGGGACGGAAGAGGCCAGAGCTCGCCTTAGCTCCCCGCCCTGTTCACCTGGTTCAAGGGCATGGGGACCCCAAACAAGGCTTCCTAGTGACAAGCTGCAAGACTCACTTCCTGATCCCCCTTGTCTTCCAGGTCCCCTTAAATCAAGGAAGGACATGGTGATGCTATCACTGAATCATAAAACCGTGGAGTGCTTGGCTGTGTAGGGGTCCAGCCCTTTCCAGCCCGGTCTGATAGCTCAGACACTTAGGTTTTGAACTGCAGGGTGAGGAGGAGCTCCCCCAAGATAGGGAAGAGTAGCCCTGCAGGGTGACTGGCTCTGCCACCCTGTGCCAGCCTCCCAGGAGAGAGGAAGAGCTCTCACCGGATGATCCCAAATTCATCGAAATAGCCCCCGCGACCCTCAGTGCCAAAGGGCTCCTTGAAGGTGAGGATAACGCAGGCGATGTTGTCCCGGTTCCAGATGGGGCCGAAGATCCTGTTGGCAAATCTGCAGGGAGGGGCAAGGTGGAGGAACTGACCTTGGGCCTCTGTGGTGCAGGGGCCACATGTGAGGGGTCACCCTTGTCTGAGTTCTGGAGGAATTCGTCCTCGGGGAGGCAGTGGGCCAGGTGAGGCTCCTGAGTACCACCCCCACCCTGGTCCCCCGGCCCAGGCTTGGCCCCACCTCAGCACCATGAGGTTCTGCACCATCTCCTTGCCCAGGTAGTGGTCGATGCGGTAGATCTGGTCCTCACGGAACAGGGAGGAGATGTGGTTGGACAGCCGGTCAGAGCTCTGCAGGTCCCTCCCGAAGGGCTTCTCCACGATGATGCGGTTCCAGCCTCTGCTGGGAGCCCGGAGCTGCGTTACCCCCTTGAACCCCTCTTCGGGGAGTGAGGATCACAGCTGCATCATTCAGACGCCCTCCCAGGGGAGTAGAGGCCAGAACCTCCTCGCCCCCGTGGCCACCTCCCGTGCCTTGTGTTCCCAGATGACCCTCTGGCTCAACACCTTCCGGGAAGCCTTCCCACCCTGGGTGCCAGGGTGGCATTGCTGGCATGCTGCCGGGGGCCCGTTCCCCTCCTGCCTCATGCTGGGTCCCCGGTGGGTCTGAGTGGCCTGAAGGCCTGTAGGGGAGCAGGGGGAGGAGGCATCCAAGCCATGGCTTCCTCATTGGTTTTGAAAATGAGAAGAGGACCTTTGCTTTACTACCCCCGCATTCAAAACCAGCCAGAGGACAAGAGCCTCTGGCTGGGTTGGGAAACCACCCAGCGCGGCCATGCTGCATTCGCAGAGCAAGGCTGCCACCCTGCGGCCTGGCCGGGCCTTTGGGGAAGCAGAGCGGAAAGGCGGTGTTTCGTGGAGCAACGCTGCCACCTTGTGGTCCCGCTGGGGATGGCCCCGGCACCATGGATGCTGCCCAGATCCCCGGCCCCGGACACGCTCATAGAGTGGTGGGAGCACTGCCTGGGCCAGCCTGGCAGGCGGGAAGGGAGGGCAACGGCAAGCCTTACATCTGGCTCATGCAGGACTCGTGAATGTTCTTGGTGACGGCCTCGTAGACGGTCGGGGGCAAGGCCAGGTAGAAGAGGCGGTTGGCCTGTGACCCCAGGTGGAGGGCATCCATGTGGCTGTTGAGGCGCTGGTAGGAGGCTGCATCATCGTACTGGCCAGCCACATAGGAGTTGCGGGCAAAGAAGTCCTCCAGCTTGAGCTTCTCCTCTGGGGTGGCCTGGGAGACACGGACAGACAGACACACAGACAGATGTCAGCCCCTCTCTTTGAGTCCGTGTGTGTTCTGCCCCAGGGGAGTGGAGGGTCTTCCCTGGAGGTCCAGGGAGGGTGCCCTCAGAAGTCAGTGTCCCCGTCCCACACTGGGTTCAGCCCCATCTTAGCAGCTCTGCACATCCAGAGGGAGGGAGGCCAAAGCAGGCAGCACAGACACTGCCCCAGGCTGGGACCCCTGTGCCTTAGGACAGAGGCCAGATTTCAGGATATTTTGACCTGGGAGAAATACACTGGAGAAAGCTCTCTCTCCAAAATCATGACACCCAACTATGATTGGCGGAGAAAACGCAGCAGAGCACAGCAGGAGGGACCTGTGGGTCCTGGTCACGGGGGCTGGTAATGGGGGTCTCAAGAAGTACGAGAGCAGGCGGGGCGGGGCAGGAGAGGAGGAGAGCATCCCGGGATGGGATGGGGGGAGGTCCCCGAAGCTGGCCATGCTGGGGGCTGGTAGAGAGGGCAGAACCAGGCTGGGGGAGGCCCTGACACCACCCACCTTGAAGAAGGGCTCACTCTGTTTGCGGATGTCAGCCACTGTGAGGCGGGAACGGGCATAGCCCATGATGAAGGTGTTTTCGGGCAGAAGGCCATCCCGGAACAGCCACCTGAGGGCAGGGCACAGCTGTAACCAGTGCGGGCAGGGCAGGACCAGGCCTGTCCCTGGCGGGAGGTCACAGGGGCAGTGGTGGGACACACTTACCAGATGGTGGGGTAGATCTTCTTCTTGGCCAGGTCACCCTGTGGCAGAGGGAACAGGTGTGTGGTTAGAAGTGGCTGGGGACACGACCTACATACATCATCCTCCACCCTTGGTGATCTGGGCACTACTCAGGATCACTACTGGGCCACAAGCATGTCTGTCTTGCCTGATACACAGACAGAAGAGCCCCGAGATTCAAGCCTGTGCCAGCCCTCCATCCCTGACCCAGAAGACATAAACGCATCTCCTGCGTTCCAGGAGAAAACCTGAAAATTCAATTCAGTAGAACCAAGGGACGACAGAAGTATCATGTAGCCACATTTGTGAGACGTGACCTCAAGTGCTTACATTAGCAAAAAAGAGTGGTTCAAAAATAAGAAGCTAAGCATTCCACTTAAGAAGTGAGAAAAAGGGCCAGATGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAAGTCAGGAGTTCAAGACCAGCCAATGTGGCAAAACCCCATCTCTATTAAAAATACAAAAATTAGCCAGGTGTGGTGGCAGGCACCTGTAATCCCAGTTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGTGACAAGAGTGAGACTTCATCTCCAAAAAAAAAGTGAGAAAAAGATAGAACTGCAAGCCAAATTACCAGGAAGAAAATAGGAAATAATGACAAGTAAAATTAATAAAATTAAAAGCTAACCCAAAGAAAAGATGAACAAAGCTGGAAATTGGTGTTTGAAAAAGACGAATAGAAACAGACAACCCCCTGGCATGGTTGCTTTTTTAAAAGGGCATAGGCCGGGCACAGTGGCTTACTCCTGTAATCCCAGCACTTTGAGAGGCCGAGGCAGGCGGATCAAGAGGTCAGGAGATCGAGACCATCCTGGCTGACACGGTGAAACCCCGCCTCTACCAAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGTGTCTGTAGTCCCAGCTACTCGGAAGGCTGAGGTAGGAGAATCACTTGAACCCGGGAGATGGAGGTTGCAGTGAGCCAAGATTGCACCATTGCACCCCAGCCTGGGCGACAAGAGTGAAACTCCGTCTCAAAAAACAAAAAACAAAAAAACAACTAAACAGCTGGACGCGGTGGCTCAAGCCTATAATCCCAGCACTTTGGAAGGCCGAGGCGGATGGATTACCTGAGTTCGGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCGTTTCTACTAAAAATACAAAATTGGCTGGGCATGGTGGCCCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATCAAGCCACTGCACTCCAGCCTGGGCTACAAAAGCGAAACTCCGTCTCAAACAAACAAAAAACAGAGCAATGGACCTGAGAGGGGACAGTGGCCACAAATCTTCCTACAGAAACAACCCCCTGGCATAGAGAGTGTTGCCAGTGGGTTCTACCAAAATGCAAGCACAAGAGAATTCCAGCCTGAAGCAAACTCTTCCTGCAGAAGGAAGAAAGGGGAACACTTCCCAAGCCACCTTATGGGGCCTACAGAGCCTTGGTACGGAAACCTGATGAGAAAGGCACATGGGAAAACCCATACGCCTCATTCACACACACATGCAGATGCAGAAATCCCACACGAAATATCAGCAGGCCGGGCACAACTCACACACCCCAGCACTTTGGGAGACCAAGGTGGGAGGAAGATTGCTTGAAATCAGGAGGTTTTTTTTTTTTTTTTTTTTTAAAGACATGGTCTCACTCTGTCACCTAGGCTGGAGTGCAGTGGCACGATCTCAGTTCACTGCAACCTCTGCCTCTGGGCTCAAGCAATCCTCCCACCTCAGCCTCTGGAGTAGCTGGGACTACAGGTACATGCCAACACACCCAGCTAATTTTTTGTATTTTTTGTAGAGACAGGGTTTCCCCATGTTGTCCAGGCTGCTCTTGAACTCCTGGGCTCAAGTGATCTGCCCGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACGACGACCCACTGACCCTGTCTCTTTAAAAAAAAAGAAGAAAACATCAGCAAACCAAATCCTGCAATGTTAAAAACGGTAGGTTTTTTATTTACAGAATAAATACATCTGTAAATAAAAAGTTGGTTTGATATTAACAGAAAAAAATCATTGAAATTCACCATATTAAGAGATTAAAAAAAACCTCTAAGAACTCTCTGCCTACTGCTATGTTTTGATTTTTTAAATCTAGCAATCTTGCTCAACCCAACAGATGTAGATCAAGTGTTTCAGAAATCACCTATTCACGCTAAAACTCTTAGCAGAGGGGGAATGCAAGGGAACTTCACTAACTTGATAAAGGGCATCTATAAAACACCATTCTGGCTGGGCGTGGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGGAATCGCTTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATGGTGAAACCCCATTTCTACTAAAAATACAAAACTTAGCTGAACATGGTGGTGTATGCCTGTAATCCCAGCTATTCAGGAGACTGAGGCAGGAGAATCGCTTGAACCAGGAGGGAGAGACTGGAGTGAGCCGAGATCACACCACTGTAGCCTGAGCAACAGAGCTAGACGTCGTCTCAAAAAAAAAAACCACCACCAAAAAACAAAGAACAAAAAAACCCCCAAACCATCATCCTTACAAATACTACAAATATCACACTTATAGGAAATGCTGAAATATTAATCATCCCTTTAAGCAATCAGGAACAAGAAAAGTGCAGTAAGGCAAGAAAATGAAAATGGCTGAAGTTGAGCTGTGAGGTGGTGCGGGTGCTCCCTCTGATCAAGTGAAACACACACCTATCCTGTGACCTGGCCGTTCCACCCCAAGAGAAATGAAAGTGCAAGTCCACACAAAGACCTGCACACGAATGGTCACAGCAGCCTTGGTTCATAGCGGCCCCAAACTGGAAACAAGTCAAACACTTGAATTAACATGTGACCCGGCAAACTGTGCATTCATACAACGGATACTACTCAGCAACACTGGGAAATCTCAGCATCAGAGATGCAACCCATTGTGACAGAAATCAGATCAGCGGTTGCCTGGAGCAGGGCAGATTGGCTGGGAATGGGTACCCAGGAACTTTCTGGGGAGATGACGATGGCCTTTATTGTGATGAGGGTGTACAGCCAATTGTACAGTTAAGATTTGCGCACCAACAAAAGACGGCGAGAAAACCAACAATGGAGCGGAGGGTGGGGAGTGGGTCAGAAATGACCCTTTGAGGGCCAGTGACTGTGAGAGCCCCAGGTGAGGGGGCTTCCTGGGTGCAGAGCATATTCTGTTTCTTGATCTAGGAGCAGTTTCTGTGGCTGTGCTGAGTTTGTGAAAAGTCATCAACAAGCTAGACCTGCACCATCTGTGTTCTTCATGACATGTATTTCTTTTTTTTTTTGAGACGGAGTCTCACTCTGTCGGCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGAAATCTCCACCTCCTGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTCCAGGCACCTGCCACCACGCCCGGCTAATTTTTGTATTTTTACTAGAGATGGGGTTTCGCCATGTTGGCCAGGCTGGCCTCAAACTCCTGACCTTGTGATCCACCTGCCGTGGCCTCCCAAAGTGCTGGCATTACAGGCGTGAGCCACCGTGGCCGGCCCATGACATATATTTCAATAAAACATGGTAAAAACTAATAGTCTGGGCGTGGTGGCTCATGCCTGTAACCCCAGCACTTTGGGAGGCTGAGGAGGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCAAACATGGTGAAACCCCGTCTCTACTAAAAATACAGGCCAGGCGCGGTGGCTCATGCCTGTAATCCTAGCACTTTGGGAGGTTGAGGTGGGTGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCCAACATGGTGAGACCCCGTTTCTACTAAAATACAAAAAATTAGCTGGATGTGGTGGTGCGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGGGAATTGCTTGAACCTCGCAGGTGGAGATTGCAGTGAGCCAAGATTGCGCCACTGTACTCCAGCCTGGTGAAAGAGCAAGACTCCATCTCAAAAAAAAGAAAAAACAAAAATTAGCTGGATGTGGTGGCAGGCACCTGGAATCCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCACTTGAATCCAGGAGACGGAGGTTGCAGTGAGCCAAGATTGCGCCACCAAACTCCAGCCTGGGTGACAGCAAGACTCCATGTCACGAAAAAAAAAAAAAAAAAACACATGCTCTTCGAAGCAATCAGGCACTGCAACCATGGATGGGTCCATTTCTACAAACCATTACAGAATGGGGGCCATGCCACCAGGTCAAACGCCCTCACCCAGAGCCAGGCAGTTAGGAAGCCACCACAGGGCTTCCAGGAGGGCAACTGCTATGGTGTGGGTGCCTTGTGTGCTTGCGCTCTTCAGGGGCCCTGCTCCACTTCTGATGCTCCTCAGGTGAGCTCGGTGCCAGGACTACAGCGACACGGACCTCTCACTGCTGCACCCTTCAAAGTCCAGCAGACAACCCCTCGCCCTGGGGTAGGAGCTCCTCTACTCAGCTCCTCACAGCACCTGCCATCAGTGGGGCTGGGGAGGGGAACGTTGCCCATGTCACATCAACTCACGGCTGGCCCCACACAGAAAGGGTCAGCTTACTGGGAACTAACTGGACCCTGAAATAAACGGAAAGAACCACAGCCTGCAAATACCTCTAAGCCTCACTCAAAGCAGGTCATGTTTGCTCATGACCAAATTCCGGAAGACTGGGAGGCATCGGGACAGACTCCAGAAAGGTACTGGTGGCAGGGGACTGGTCTGCTGAGTCACAGCAACCCCACGCTCCAGGCCGTTTGTTCCTGCTGCTCCCTTTGGGATTAACTAGAGATTGAACCGGCTCACCTTGTTTCAGCTCAGGCCCTGCAACCGACCGACCGTCTGCTGGGCTCCAAGTCACCTGAGAGGCGAGGTGAGGTAAGACGGACAGTAGGGGTGGGGTAAGGCAGGCCGCTCTCTCCCATGGTGACAGGGAGCTAAGCTGAGCTGAGCCGGACTGAGCTGCTCTCCTCAGGACTCAGGGGTAGCCCTGCCCTCCCAGCTGGCTGCGATGCTCTAGTGGGACTTTCTGGAGGAACAAACATGGGAAGACATCGGGAAACCTGACAGCTTGGTCAAGAGTCTACTCGTGCCATGTGGGTGGGGGCAATTCAAGGGGTCCTTAGTGAAAAGCAGTTGGGAGCTTCTCATCTTCCCCACGCCCTCCTCTTTCCTGAGGACCACAGCAGGGGAGGGATCTGCCCAAGGACACAAGGTGACTTAGTAGAAGCAGGGCATGATGAAGATCTCTAGCCACCAACATCTGGCTAGGCCAGGGCAAAGGGACCTGGGCCATGACCTGTCTGTTTTATAAACCGAAATCGCTGCAGCCCTTGTCTTGGCATGAGGGTTTCATGGTAGAGCACGATGCCTGCTGGAGGGCCAGCGGGGGTGGGCCATCCCATCCCCAAGGGTCACTGGGGTTAAGGACCTGCCCAGACTCCCTGGGTGGAGCCCTGGGCCTTGGCAGGGGCCCAGCTGGTTCCTGGCCACTGGGGCCAGAGAACCTGGGGGCTGCTGAGTTAGTTCAGCTGAAAAACCAACAAAAGGGCCTCCCTGCTATACCCGGGGGCTCATGAGTCACCGGGGCTTTGGGGGAGTGCCAACATCATCATGACACAGCAAGAATCTTATCAGACCAATGGGGAAGTCAGCCCAGAAATGTTCTGAGGAAAGGGGAGGCGGGGGCCGCTGGGTCATCCCTCCCACAGGCCCATCATTGGGATGCGTCCTGAACGCCCATCAAGCCCATGGCCCTTGTGATCCAGGTGGGGAAACTAAGGCCCAGAGAAGTGAGGACCCCGCAGACTATCAATCCCAGTCTCTTCCCCTCACTCCCTGTGAAGCTCTCCAGCATCATCGAGGTCCCATCAGGTGGGGAAAGATGCTGTTCCAGGCGCACACTAGTCTACAAGGCCAGAGCTTTCTGGAAGGGGGCAGTAAGTACCTCGGCTCCCTTTCTGGTAGGGGTGGGAGTCCTGAGAAGGCAGGAAGTGGCCCACTTGGTAACTCTGAGGTGCCATCAGGGCCCCCAGGAAGGAAGCTGGGTGTGTGGGCAAGTGTGAGGTAAGCTGGCCAGGGAGGAGGAAGGGACAGAGGAAGGCCACGTGGGTCCAGCCTGCCCCAGGGTGTCCTGCTTGCCCAGGCTGTGGGTCTGCCAGCCACTTGCCTGCTTTCAGTTTCTAGGTCATGCTGAGCTTGTTCCCAACTCGGGGCTCCGGGCATTTGCTCTTCCTTCTGTGTTTGTGCTCTCCCGGCCCTCTTTGTGGGATCTATGCGCTTTGGGGGACTGGGGACACAGGGCCCATGTGTATCTTCTGAAACACACGTCAGCCTGAACTCTTGCTGGTCTGCTTACTTGCCGTGGTTCCCTGGCCGCAATAGCACATAGGTCCCATGAGGAGGGCAGGGACTGGCTGCTGTGGCCCAGAAGTACCCAATCGCTGTCTGCTCAATGAACGGAGAATGGGCTGCTTTCCTGGAACAGCAGATTCTAGGATCACGTGCCCTCAAGTGCCACCCTGCCTACCTCCCGCACCGAGTGAGGCATCAGGCGTGGAAGAAGCCTGGGAGCCGGAGCTGTTCCAGGTGCTGCCTGAGCACGCCACCTCCCATCTCCCCCACAGCAGGAAGAGGAAAAAACAAACCACGAGGCTCTTCAGAGAGAGGACCCCTTGTCCCCTACCCACAGTGCTGGAGCTGGCACTTCCTATTTCTGCTTTGAAAGCCTCAGGTTGTCACTCTCAGAACAGAGGAGAGCAAAGGGGAACCCTACTGATTTCAACAAAACAAAGTTGCCCAACCCGAAGCTGGCCACAGGCGGAGGCAGTGAAATGACAAAATCAGCTTGGAAAAACCTAAGGACCCTGGGCCCTCGTTTCAAAAGCTGTCATTTGCTACACGAAATGCTGAGGTTCAGGAAAAGGAAGACACTTGCTCCAACTCACACAGCAAGCTTGGATGCTCTCAATGAGGTGTCTAATAAGAGCTGAAGGCCAGGAGTCATAAGCTATCATTGTGGCCTGGGTTGCTCTGTTGCAGTGCCTTGTGACAGCATGGGGTGGGGATGGAGAAAGCAACCTCAGTTACCTTCTCCTCCAGTCTGCTTCTTGGACTAAGGGTTTAACGGTCAGAGTCCTGGCTGTTAAGGTTTGTGGCTGATGCAGGTATGGCTCTTTTTATTTTTATTTTTTTGAGATGGAGTCTCACTCACTCCGTCACCCAGGATGGAGTGCAATGGTGCCATCTCAGCTCACTGCAACCTCCACCTCCTGGGTTCAAGCGATTTTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGATGGGTGCCACTACACCTAACTAATTTTTGTTATTTTTTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCACCCAGGCTGGAATGCAGTGTTGCGATTTCTGCTCACTGCAAGCTCCGCCTCCCGAGTTCACGCCTTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGACTACAGGTGCCCACACCAGGCCTGGCTAATTTTTTATTTAGTAGAGACGGGATTTCACCATGTTAGCCAGGATGGTCTCAATCTCCTGACCTCATGATCCACCCGCTTCAGCCTCCCAAAGTGCTGGGATTACAGACGTGAGCCACTGCGCCCGGCCTTTTTGTTTTGTTTTGTTTTGAGACAGAGTCTCGCTCTGTCGCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAACCTCTGACTCCCTGATTCAAGCGATTCTACTGCCTCAGCCTACTGAGTAGCTGGGACTACAGGCACGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCTTGACCTTGTGATCTGCCCACCTTGGCCCCCCAAAGTGCTGGGATTACAGATGTGAGCCACTGCGCCCAGCCAATTTTTGTATTTTAAGTAGAGACTGGGTTTCGCCATGTTGGCCAGGCTGGTGTCAAACTCCTGACCTCAGGCGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGAATACAGGCGTGAGCCACCGTGCCCGGCCAGGTATGGCTCTTCTGAGGGGACCAGGCTGGGGCTGGGGCTGAGGCCAAGCCCAATCTACTGTGGGCTCCACCTGGTACCTCTCCTGGGTCTCAGGCTTATGGGGAGTCAGAGGACAATGGCCCCTCCTTACTCTGCCACTGGCAGAGCCCTTCTCCCTCGGCTGCCTCCTCATTCCCTTTTGGCTCTCCTTTTCTAAGTTCTGATCAGAAGTACAAAGGTGTCAAGGAGTAGGTTTGACAAAGTGTGACAGCGCGTTGTTCTATGTGAACAAAGAACCACTGAGCTCAGCCAGCACTGAGGGGCGCACGATGTGGAAGAACTAACTAGTTTTGATAGAGCTCCTGCTCAAGGTTACAAGGTAAGTTAATGGCAAAGATGGTCATACAGTAATGAGCAGAGAGATTCTTGAGTGCATCCAGGGTTAAAAGTGAAAACTGAGACCACGTGTAATTTGAGATGAAGCCCTTGTTCCACCAGCCCCTGCACAGTCTCACTGCCCCATGGGACACAGGGGAGGGTGCTCTAGTCTCGGGCGATGGCTGTCCTAGGACCACCCCTCCCTCCCCTCCCATGGAAATCCTCATGCTATACTATTCTTTTGTCTTTCCTATGAGTGCAGAATGGCGGTTCTACAAGCTGGACAATTGGGGCCAGGTGGGGTGAGGCAGAGCTCCTTGGTAGGCTTTCGAAATTGAGGCAAAGAGACAGTGTTCAAAGAAAAGCTAAGTGTTTGTATCGACGGAACTTGAAGTGTTAGTGAAGAGGCAGAGATCAGGCCTCAGATCCTGCCTTTGGAACTCATTTGTTAAGGCATGAACAGGTCTGAAAAAACTACAGAATGAATAGCATTCCCTGTTTTCCCCAAGAAGTCCATCTAGACAGTCCCTAAAGAGCCTGCAACTCCAGGATTAAGGGCTACATTCAGCGGCTAGGCACAGGGTTCAGAAACGTCCTGCAGCCGGGCGTGGTGGCTCATGCCTGTCATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGTCAACATGGTGAAACTCCGTCTCTACTAACAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCTCAGCTACTTGGGGGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCCGAGGTTGCAGTGAGCCAAGATGGCGCCACTGCACCCCATCCTGGGCGACCAGAGCAAAACTCCGTCTCCAAAAAAAAAAAAAAAAAAAAGTCTTGCAAGTGCATATGCACACCAGGTAGAGCCGGGATGATCCTGGCGCACTAGCAGGAGCGGGAGGAGGAGCTCAACTTAGCAGAGCCTGTGGGGCCCTGCAACAATTAGTTGGAAAAGCTGAGGCATGGAGCAGGCACTTCCTGGCTTTTAAGATTGGGGCCTGGGAGATACTCACCGATGCACCCATGATGATGAATATGTGTGTATCCGACTGATGGAAGGCATCGCCCTGGAAAAGCTCTTCCCGCAGGATCCCGCACACCTGGGTCCGGCTCAGGGCCACCTGCTCTGCCATGACGCTGTCTGGTGGAAGAAAGGCTCGTTAACAAGGCAGAAGAACAGGAGAGCATTGAGAAGTTAGCCCCTTTCTTGAGAGTTCCTCTGGGGTCTCACACCAGGGTGACACCTGATTGCCCAAATCACTCCTTGTGAACGGCTGGGCATTGGGGAGTGGTTGATGGGTTAGAAACTGCTGGCTCTGGGCTCCAGCCACTCTCTCCTAGGCAGCCTTCATTCAGGGTGTACATTACAAAATTACTCAAAGCATTGGTGTATTCCGACTACAGCATCAATTCTGGACAACCGAGTAAAATCCTTGTGGGGCATGGAACTGCGTGCCCAGGACCATCTCATTCCCGACTGTAGCGGGAACTCCACAATGACCTGGAGCATGGGAGATGGTGCAGATGCTGGAGTTCAGCTCCAGCCCTCCCCTTGCCAAGCTGGGTGACCCCGGTCGGCAAGTCCCCTTCGCTCTCGGGGTCTCAGGGGCTCAAGAGAGGAGGTGCGGGGTATAAAGGGATTGGTTAAGACCCTCTCGATTCTGCTCGGTTCTCAAGCACAACAAACAGCGTGTATTTTACCGCCGCGCGGCGCAGCGCGGGACAGTACGCTCCTCCGCCTGCGCGGCGCCCGCCCGGCCGGTTACCTGCGCTTCGTCGTCGTCGCCCTCCGCGCTCGCAGCCCCGAAGTGTACGACCGTTTCCGGGGGCTGAGCCCCGCCGGCCCATTTAATCGGCGGGGGCGGGGGCGGGCGCCTGGGCTGAGCGGACCCGCCTCAGGCGAGGCGTGCGGGGCGGGGCCTCGGCCACCACCCCTCGTGCGGGCGGGGCGGGGCGAGGGCAGGTGCGCGCGCATCCCAGGCCAGCCCCTGCCTCTCGGGCACCTGCGCTGGAGGCCGGCCCGCCGGCTGCCTGCCATACCCGCTGCCGCTGCTCTGCATCCCCAATTCCGGCGGGCACGGGTGCAGCTCCGCGTAGTGCTCCCGCATCCCCATCGCCGGCCCGGCCCCGCCCCTACTGTCCGGTTTCCCCGCCTGCCCCGGCGGCCTCGCGCGCTCGCGGAGGGCTCCACTTCCGCCGGCAGCGTGGCCACGCCTCCTCTGGGCTGTCCCTCGGCTCCTGGGCGGGGCCTTCTGCCGCGCCACTCCCCCGGGAACCCTCGCCTGTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCGAGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGACTTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCACAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCCGCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGGTGTGGTCCTTTTCCCCAAAGACAGGGTTCCATCCGTGGGCGTTCCGCCGCCTCCGAAACTTCCCCCGGACGTTCAGGCTCCCCCCTCTTTTTTGGGCCCCAGCCCGTTCCTGCTCCGCGCTTCTGGAGCACTGGCCAAGGCGGGCCGATTCAGGACCCAGGTTACTTGGGCGGCGAGCTGGACTGTTTCTACTCCTCCCTCCTCCTCCACTGCGGGGTCTGACCCTACTCCTTGTGTGAGGACTCCTCTAGTTCAGAGACATATTCTGTTCACCAAACTTGACTGCGCTCTATCGAGGTCGTTAAATTCTTCGGAAATGCCTCACATATAGTTTGGCAGCTAGGTATCTGATTTCATATGCCTGTTTGCTCGTTTTGCAAGACAACATCTGCCTATCGTCATACTGTTTCTGTGATCTGAGAATGAATGGGCTCTCCTGGCACATTAGAGGAATAGCACGGAGGTCACTATAGCTGGAGCAGAGTGAGGGAGGGGAGCACAGTTGGAGAGGAAGGGATGGGGAACCCGATGGTACAGGGCCTTGTAGGCCGTTGTAGGGAGATTGGCTTTTACGCAGAAGGCAACGGGGATCCATGGTAGGGTTCTCAGTAGAGGAGGAATGTGATCGGAATTACGTCTTACACTGATCGTTCTAGCAGTGGTGGGGAGACCAGACCATAGTGGGCAAGGGTAAAAGAAGGGTGGCGTGGTAAGAGGTAATTGCAGTAATCCAGCTCAGAGACAGTGCTTTGAGCATTTTATTATTGGAAATTTCAAAAATGTACGAAAGTAGAAAAATGAGTATAGTAAATCTTTTTGGTTTGTTTGTTTTTGAGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTCTAACCTCCTCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCTTCCCAGGTAGCTGGGACTGCAGGCGTGCGCCACTACGCCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACTATATGTTGGCCAGTCTGGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGACTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCGCTCGGCCAGTATAGTAAATCTTACATTCACAGCTACCAACTTCAACAATTGAAGGTATCAACTTCTGACTAATTTTGTTTATATCCCTCGCAATCCTCTCCCATTATTATTTTATTTCTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTTTTTGAGACAGAATCTCGCTCTGTCACCAGGCTGGAGTGCAGTGGTGTGATCTCCACCCACTGCAACTTCCGCCTCCTGGATTCAAGCGATTCTTCTGCCTCAGCCGCTGGGACTACAGTTGTGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCATCATGTTGGCCAGGATGGTCTTGATCTCTTGACCTTGTCATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCGGCCTCTTTTTTTGAGACCCAGTCTCACTCTGTCGCCCAGAATGGAGTGCAGTGGCACGATCTTGGCTCACCGCAACCTCCATCTCCCAGGTTTGAGCGATTCTCCTGCCTCAGCTTCCCAAATAGCTGGGATTACAGGCATGCACCATCACTCCCAGCTATTTTTTTTTTTTTTGTATTTTTGGTAAATACAGGGTTTTGTCATGTTGGCCAGGCTGGTCTTGAATTC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.029383 PRIMER_LEFT_0_PENALTY=0.016035 PRIMER_RIGHT_0_PENALTY=0.013348 PRIMER_LEFT_0_SEQUENCE=CCTGTAATCCCAGCTATTCAGGAGACT PRIMER_RIGHT_0_SEQUENCE=TCTTGCCTTACTGCACTTTTCTTGTTC PRIMER_LEFT_0=46922,27 PRIMER_RIGHT_0=47193,27 PRIMER_LEFT_0_TM=65.016 PRIMER_RIGHT_0_TM=64.987 PRIMER_LEFT_0_GC_PERCENT=48.148 PRIMER_RIGHT_0_GC_PERCENT=40.741 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.1000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=272 PRIMER_PAIR_1_PENALTY=0.037967 PRIMER_LEFT_1_PENALTY=0.019571 PRIMER_RIGHT_1_PENALTY=0.018396 PRIMER_LEFT_1_SEQUENCE=ATTCCCTTTTGGCTCTCCTTTTCTAAG PRIMER_RIGHT_1_SEQUENCE=GCTCATTACTGTATGACCATCTTTGCC PRIMER_LEFT_1=52888,27 PRIMER_RIGHT_1=53112,27 PRIMER_LEFT_1_TM=65.020 PRIMER_RIGHT_1_TM=65.018 PRIMER_LEFT_1_GC_PERCENT=40.741 PRIMER_RIGHT_1_GC_PERCENT=44.444 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.0000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=225 PRIMER_PAIR_2_PENALTY=0.045517 PRIMER_LEFT_2_PENALTY=0.029770 PRIMER_RIGHT_2_PENALTY=0.015747 PRIMER_LEFT_2_SEQUENCE=ATTGAGGCAAAGAGACAGTGTTCAAAG PRIMER_RIGHT_2_SEQUENCE=CTGTTCATGCCTTAACAAATGAGTTCC PRIMER_LEFT_2=53408,27 PRIMER_RIGHT_2=53537,27 PRIMER_LEFT_2_TM=65.030 PRIMER_RIGHT_2_TM=64.984 PRIMER_LEFT_2_GC_PERCENT=40.741 PRIMER_RIGHT_2_GC_PERCENT=40.741 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=130 PRIMER_PAIR_3_PENALTY=0.045517 PRIMER_LEFT_3_PENALTY=0.029770 PRIMER_RIGHT_3_PENALTY=0.015747 PRIMER_LEFT_3_SEQUENCE=ATTGAGGCAAAGAGACAGTGTTCAAAG PRIMER_RIGHT_3_SEQUENCE=CCTGTTCATGCCTTAACAAATGAGTTC PRIMER_LEFT_3=53408,27 PRIMER_RIGHT_3=53538,27 PRIMER_LEFT_3_TM=65.030 PRIMER_RIGHT_3_TM=64.984 PRIMER_LEFT_3_GC_PERCENT=40.741 PRIMER_RIGHT_3_GC_PERCENT=40.741 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=131 PRIMER_PAIR_4_PENALTY=0.056639 PRIMER_LEFT_4_PENALTY=0.043292 PRIMER_RIGHT_4_PENALTY=0.013348 PRIMER_LEFT_4_SEQUENCE=CCCCAAACCATCATCCTTACAAATACT PRIMER_RIGHT_4_SEQUENCE=TCTTGCCTTACTGCACTTTTCTTGTTC PRIMER_LEFT_4=47083,27 PRIMER_RIGHT_4=47193,27 PRIMER_LEFT_4_TM=65.043 PRIMER_RIGHT_4_TM=64.987 PRIMER_LEFT_4_GC_PERCENT=40.741 PRIMER_RIGHT_4_GC_PERCENT=40.741 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=5.3000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=111 = primer3-2.3.6/test/primer3_v1_1_4_default_settings_output0000664000077300007730000002050512231156245022460 0ustar ioanaioanaP3_SETTINGS_FILE_USED=../primer3_v1_1_4_default_settings.txt P3_FILE_TYPE=settings P3_FILE_ID=Default settings of primer3 version 1.1.4 PRIMER_TASK=pick_detection_primers PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=5 PRIMER_PRODUCT_SIZE_RANGE=100-300 PRIMER_PRODUCT_OPT_SIZE=0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_MIN_SIZE=18 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_MAX_SIZE=27 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_MIN_GC=20.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_OPT_GC_PERCENT=50.0 PRIMER_MAX_GC=80.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_GC_CLAMP=0 PRIMER_MAX_END_GC=5 PRIMER_MIN_TM=57.0 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_MAX_TM=63.0 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_PAIR_MAX_DIFF_TM=100.0 PRIMER_WT_TM_LT=1.0 PRIMER_INTERNAL_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PRODUCT_MIN_TM=-1000000.0 PRIMER_PRODUCT_OPT_TM=0.0 PRIMER_PRODUCT_MAX_TM=1000000.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_TM_FORMULA=0 PRIMER_SALT_MONOVALENT=50.0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=0.0 PRIMER_INTERNAL_SALT_DIVALENT=0.0 PRIMER_DNTP_CONC=0.0 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_SALT_CORRECTIONS=0 PRIMER_DNA_CONC=50.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_MAX_SELF_ANY=8.00 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_WT_SELF_ANY=0.0 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_MAX_SELF_END=3.00 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_WT_SELF_END=0.0 PRIMER_INTERNAL_WT_SELF_END=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_MAX_END_STABILITY=100.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_MAX_POLY_X=5 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_MIN_THREE_PRIME_DISTANCE=-1 PRIMER_PICK_ANYWAY=0 PRIMER_LOWERCASE_MASKING=0 PRIMER_EXPLAIN_FLAG=0 PRIMER_LIBERAL_BASE=0 PRIMER_FIRST_BASE_INDEX=0 PRIMER_MAX_TEMPLATE_MISPRIMING=-1.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=-1.00 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=24.00 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_MIN_QUALITY=0 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_MIN_END_QUALITY=0 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_QUALITY_RANGE_MAX=100 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_INSIDE_PENALTY=-1.0 PRIMER_OUTSIDE_PENALTY=0.0 PRIMER_WT_POS_PENALTY=1.0 PRIMER_SEQUENCING_LEAD=50 PRIMER_SEQUENCING_SPACING=500 PRIMER_SEQUENCING_INTERVAL=250 PRIMER_SEQUENCING_ACCURACY=20 PRIMER_WT_END_QUAL=0.0 PRIMER_INTERNAL_WT_END_QUAL=0.0 P3_SETTINGS_FILE_END= SEQUENCE_ID=test_settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.013026 PRIMER_LEFT_0_PENALTY=0.006937 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_0=198,20 PRIMER_RIGHT_0=315,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.006 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=118 PRIMER_PAIR_1_PENALTY=0.020508 PRIMER_LEFT_1_PENALTY=0.014419 PRIMER_RIGHT_1_PENALTY=0.006089 PRIMER_LEFT_1_SEQUENCE=ttctctggacacagctccct PRIMER_RIGHT_1_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_1=39,20 PRIMER_RIGHT_1=315,20 PRIMER_LEFT_1_TM=59.986 PRIMER_RIGHT_1_TM=60.006 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=41.16 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=277 PRIMER_PAIR_2_PENALTY=0.020508 PRIMER_LEFT_2_PENALTY=0.014419 PRIMER_RIGHT_2_PENALTY=0.006089 PRIMER_LEFT_2_SEQUENCE=cttctctggacacagctccc PRIMER_RIGHT_2_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_2=38,20 PRIMER_RIGHT_2=315,20 PRIMER_LEFT_2_TM=59.986 PRIMER_RIGHT_2_TM=60.006 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=41.16 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=278 PRIMER_PAIR_3_PENALTY=0.021356 PRIMER_LEFT_3_PENALTY=0.014419 PRIMER_RIGHT_3_PENALTY=0.006937 PRIMER_LEFT_3_SEQUENCE=ttctctggacacagctccct PRIMER_RIGHT_3_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_3=39,20 PRIMER_RIGHT_3=217,20 PRIMER_LEFT_3_TM=59.986 PRIMER_RIGHT_3_TM=59.993 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=41.16 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY_TH=8.80 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=179 PRIMER_PAIR_4_PENALTY=0.021356 PRIMER_LEFT_4_PENALTY=0.014419 PRIMER_RIGHT_4_PENALTY=0.006937 PRIMER_LEFT_4_SEQUENCE=cttctctggacacagctccc PRIMER_RIGHT_4_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_4=38,20 PRIMER_RIGHT_4=217,20 PRIMER_LEFT_4_TM=59.986 PRIMER_RIGHT_4_TM=59.993 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=41.16 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY_TH=8.80 PRIMER_PAIR_4_COMPL_END_TH=6.50 PRIMER_PAIR_4_PRODUCT_SIZE=180 = primer3-2.3.6/test/primer_all_settingsfiles_input0000664000077300007730000000326412231156245021275 0ustar ioanaioanaSEQUENCE_ID=Load qPCR settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_TASK=pick_detection_primers = primer3-2.3.6/test/primer_check_input0000664000077300007730000001014112231156245016627 0ustar ioanaioanaSEQUENCE_ID=00 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_INTERNAL_MIN_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_INTERNAL_MAX_SELF_ANY=100 PRIMER_INTERNAL_MAX_SELF_END=100 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_INTERNAL_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=02 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG PRIMER_PICK_INTERNAL_OLIGO=0 = SEQUENCE_ID=03 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=04 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=05 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=06 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=07 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT PRIMER_PICK_INTERNAL_OLIGO=0 = SEQUENCE_ID=08 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT PRIMER_PICK_INTERNAL_OLIGO=0 = SEQUENCE_ID=09 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=10 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=11 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAATTTGATTG SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=12 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=0 = SEQUENCE_ID=13 SEQUENCE_TEMPLATE=nncacgtcaTCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=cacgtcatctcatcaaatttgattg SEQUENCE_PRIMER_REVCOMP=gaattgtttggaaatgacagtgagt PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=14 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATctcatcaaattTGATTG SEQUENCE_PRIMER_REVCOMP=gaattgtttggaAATGACAGTGAGT PRIMER_PICK_INTERNAL_OLIGO=1 = primer3-2.3.6/test/primer_end_pathology_input0000664000077300007730000000205312231156245020411 0ustar ioanaioanaPRIMER_OPT_SIZE=20 PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000 PRIMER_MAX_GC=80.0 PRIMER_MAX_SIZE=25 PRIMER_MAX_TM=63.0 PRIMER_MIN_SIZE=18 PRIMER_MIN_GC=20 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=6.99 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=6.99 SEQUENCE_ID=14.RHAP64FE2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT SEQUENCE_INCLUDED_REGION=19,432 SEQUENCE_TARGET=44,46 SEQUENCE_TARGET=92,33 SEQUENCE_TARGET=140,26 SEQUENCE_TARGET=170,14 SEQUENCE_TARGET=211,20 SEQUENCE_TARGET=238,26 SEQUENCE_TARGET=336,14 = primer3-2.3.6/test/p3-tmpl-mispriming_output0000664000077300007730000006666712231156245020075 0ustar ioanaioanaSEQUENCE_ID=template_mis_1 PRIMER_NUM_RETURN=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.934085 PRIMER_LEFT_0_PENALTY=0.323851 PRIMER_RIGHT_0_PENALTY=0.610234 PRIMER_LEFT_0_SEQUENCE=Gatgcgtacgatccatgcta PRIMER_RIGHT_0_SEQUENCE=tagcggcctagtgtgtgtgt PRIMER_LEFT_0=20,20 PRIMER_RIGHT_0=131,20 PRIMER_LEFT_0_TM=59.676 PRIMER_RIGHT_0_TM=59.390 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.5000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=6.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=6.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=112 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=12.00 = SEQUENCE_ID=template_mis_2 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTG PRIMER_WARNING=More than one position in template for input oligo TCCTACAGCTGTGGGAAAATGat PRIMER_RIGHT_EXPLAIN=considered 1240, low tm 698, high tm 374, high any compl 13, high end compl 34, ok 121 PRIMER_PAIR_EXPLAIN=considered 242, ok 242 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=2600.116621 PRIMER_LEFT_0_PENALTY=0.102704 PRIMER_RIGHT_0_PENALTY=0.013916 PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat PRIMER_RIGHT_0_SEQUENCE=gatatCAGTGTGTACATGGCGG PRIMER_LEFT_0=32,23 PRIMER_RIGHT_0=197,22 PRIMER_LEFT_0_TM=62.054 PRIMER_RIGHT_0_TM=60.278 PRIMER_LEFT_0_GC_PERCENT=43.478 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.5000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=23.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=17.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=166 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=26.00 = SEQUENCE_ID=template_mis_3 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCC PRIMER_WARNING=More than one position in template for input oligo TCCTACAGCTGTGGGAAAATGat PRIMER_PAIR_EXPLAIN=considered 2, ok 2 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=2601.096874 PRIMER_LEFT_0_PENALTY=0.102704 PRIMER_RIGHT_0_PENALTY=0.994170 PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; PRIMER_RIGHT_0_PROBLEMS= GC content too high; Temperature too high; Contains too-long poly nucleotide tract; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG PRIMER_LEFT_0=32,23 PRIMER_RIGHT_0=240,18 PRIMER_LEFT_0_TM=62.054 PRIMER_RIGHT_0_TM=79.883 PRIMER_LEFT_0_GC_PERCENT=43.478 PRIMER_RIGHT_0_GC_PERCENT=100.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.5000 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=23.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=3.0000 PRIMER_PAIR_0_COMPL_ANY=2.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=209 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=26.00 = SEQUENCE_ID=template_mis_4 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACSCCCCCCCCCCCCCCCCA PRIMER_WARNING=Unrecognized base in input sequence; More than one position in template for input oligo TCCTACAGCTGTGGGAAAATGat PRIMER_PAIR_EXPLAIN=considered 2, ok 2 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=2701.096874 PRIMER_LEFT_0_PENALTY=0.102704 PRIMER_RIGHT_0_PENALTY=0.994170 PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; PRIMER_RIGHT_0_PROBLEMS= GC content too high; Temperature too high; Contains too-long poly nucleotide tract; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG PRIMER_LEFT_0=23,23 PRIMER_RIGHT_0=231,18 PRIMER_LEFT_0_TM=62.054 PRIMER_RIGHT_0_TM=79.883 PRIMER_LEFT_0_GC_PERCENT=43.478 PRIMER_RIGHT_0_GC_PERCENT=100.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.5000 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=23.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=16.0000 PRIMER_PAIR_0_COMPL_ANY=2.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=209 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=27.00 = SEQUENCE_ID=template_mis_5 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACTCCCCCCCCCCCCCCCCA PRIMER_WARNING=More than one position in template for input oligo TCCTACAGCTGTGGGAAAATGat PRIMER_PAIR_EXPLAIN=considered 2, ok 2 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=2701.096874 PRIMER_LEFT_0_PENALTY=0.102704 PRIMER_RIGHT_0_PENALTY=0.994170 PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; PRIMER_RIGHT_0_PROBLEMS= GC content too high; Temperature too high; Contains too-long poly nucleotide tract; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG PRIMER_LEFT_0=23,23 PRIMER_RIGHT_0=231,18 PRIMER_LEFT_0_TM=62.054 PRIMER_RIGHT_0_TM=79.883 PRIMER_LEFT_0_GC_PERCENT=43.478 PRIMER_RIGHT_0_GC_PERCENT=100.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.5000 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=23.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=16.0000 PRIMER_PAIR_0_COMPL_ANY=2.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=209 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=27.00 = SEQUENCE_ID=template_mis_6 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 PRIMER_PRODUCT_SIZE_RANGE=36-300 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCACCCCCCCCACCCCCCCCCCCCCCCCCC PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=1901.096874 PRIMER_LEFT_0_PENALTY=0.102704 PRIMER_RIGHT_0_PENALTY=0.994170 PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; PRIMER_RIGHT_0_PROBLEMS= GC content too high; Temperature too high; Contains too-long poly nucleotide tract; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG PRIMER_LEFT_0=0,23 PRIMER_RIGHT_0=67,18 PRIMER_LEFT_0_TM=62.054 PRIMER_RIGHT_0_TM=79.883 PRIMER_LEFT_0_GC_PERCENT=43.478 PRIMER_RIGHT_0_GC_PERCENT=100.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.5000 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=3.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=16.0000 PRIMER_PAIR_0_COMPL_ANY=2.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=68 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=19.00 = SEQUENCE_ID=template_mis_7 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 PRIMER_PRODUCT_SIZE_RANGE=36-300 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=1276.096874 PRIMER_LEFT_0_PENALTY=0.102704 PRIMER_RIGHT_0_PENALTY=0.994170 PRIMER_LEFT_0_PROBLEMS= Similarity to self too high; PRIMER_RIGHT_0_PROBLEMS= GC content too high; Temperature too high; Contains too-long poly nucleotide tract; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATGat PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG PRIMER_LEFT_0=0,23 PRIMER_RIGHT_0=67,18 PRIMER_LEFT_0_TM=62.054 PRIMER_RIGHT_0_TM=79.883 PRIMER_LEFT_0_GC_PERCENT=43.478 PRIMER_RIGHT_0_GC_PERCENT=100.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.5000 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=3.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=9.7500 PRIMER_PAIR_0_COMPL_ANY=2.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=68 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=12.75 = SEQUENCE_ID=template_mis_8 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=11 PRIMER_MAX_POLY_X=40 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 PRIMER_PRODUCT_SIZE_RANGE=36-300 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC P3_COMMENT=Should succeed PRIMER_LEFT_EXPLAIN=considered 75, too many Ns 34, low tm 14, high any compl 7, ok 20 PRIMER_PAIR_EXPLAIN=considered 20, high any compl 11, high end compl 5, ok 4 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=1076.079233 PRIMER_LEFT_0_PENALTY=0.085063 PRIMER_RIGHT_0_PENALTY=0.994170 PRIMER_LEFT_0_SEQUENCE=CCCCCCCCAAAAAAAAAA PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGG PRIMER_LEFT_0=32,18 PRIMER_RIGHT_0=67,18 PRIMER_LEFT_0_TM=61.701 PRIMER_RIGHT_0_TM=79.883 PRIMER_LEFT_0_GC_PERCENT=44.444 PRIMER_RIGHT_0_GC_PERCENT=100.000 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=4.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=9.7500 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=36 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=10.75 = SEQUENCE_ID=template_mis_9 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=9 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 PRIMER_PRODUCT_SIZE_RANGE=36-300 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC P3_COMMENT=Should succeed PRIMER_LEFT_EXPLAIN=considered 75, too many Ns 34, low tm 14, high any compl 7, ok 20 PRIMER_RIGHT_EXPLAIN=considered 177, too many Ns 22, high template mispriming score 5, ok 150 PRIMER_PAIR_EXPLAIN=considered 3004, unacceptable product size 2784, high any compl 199, ok 21 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=776.116327 PRIMER_LEFT_0_PENALTY=0.085063 PRIMER_RIGHT_0_PENALTY=1.031264 PRIMER_LEFT_0_SEQUENCE=CCCCCCCCAAAAAAAAAA PRIMER_RIGHT_0_SEQUENCE=GGGGGGGGGGGGGGGGGGTTTTT PRIMER_LEFT_0=32,18 PRIMER_RIGHT_0=67,23 PRIMER_LEFT_0_TM=61.701 PRIMER_RIGHT_0_TM=80.625 PRIMER_LEFT_0_GC_PERCENT=44.444 PRIMER_RIGHT_0_GC_PERCENT=78.261 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=4.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=6.7500 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=36 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=7.75 = SEQUENCE_ID=template_mis_10 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_TEMPLATE_MISPRIMING=6.7 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_LIBERAL_BASE=1 PRIMER_PRODUCT_SIZE_RANGE=36-300 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC P3_COMMENT=Should fail PRIMER_LEFT_EXPLAIN=considered 75, too many Ns 34, low tm 14, high any compl 7, high template mispriming score 2, ok 18 PRIMER_RIGHT_EXPLAIN=considered 177, too many Ns 22, high template mispriming score 19, ok 136 PRIMER_PAIR_EXPLAIN=considered 2718, unacceptable product size 2710, high any compl 8, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=template_mis_11 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_left_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_TEMPLATE=aTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGGc P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.732142 PRIMER_LEFT_0_SEQUENCE=cTTGATGGAGAATTGCGCAGAGGGGc PRIMER_LEFT_0=25,26 PRIMER_LEFT_0_TM=74.643 PRIMER_LEFT_0_GC_PERCENT=57.692 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=20.0000 = SEQUENCE_ID=template_mis_12 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_left_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_INCLUDED_REGION=25,26 SEQUENCE_TEMPLATE=aTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGGc P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo plus former error in ignoring included_region offset PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.732142 PRIMER_LEFT_0_SEQUENCE=cTTGATGGAGAATTGCGCAGAGGGGc PRIMER_LEFT_0=25,26 PRIMER_LEFT_0_TM=74.643 PRIMER_LEFT_0_GC_PERCENT=57.692 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=20.0000 = SEQUENCE_ID=template_mis_13 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_left_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_TEMPLATE=cTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGc P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.732142 PRIMER_LEFT_0_SEQUENCE=cTTGATGGAGAATTGCGCAGAGGGGc PRIMER_LEFT_0=0,26 PRIMER_LEFT_0_TM=74.643 PRIMER_LEFT_0_GC_PERCENT=57.692 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=22.0000 = SEQUENCE_ID=template_mis_14 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_right_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_TEMPLATE=GCCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAT P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_11 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.732142 PRIMER_RIGHT_0_SEQUENCE=CTTGATGGAGAATTGCGCAGAGGGGC PRIMER_RIGHT_0=25,26 PRIMER_RIGHT_0_TM=74.643 PRIMER_RIGHT_0_GC_PERCENT=57.692 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=20.0000 = SEQUENCE_ID=template_mis_15 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_right_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_TEMPLATE=GCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAG P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_13 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.732142 PRIMER_RIGHT_0_SEQUENCE=CTTGATGGAGAATTGCGCAGAGGGGC PRIMER_RIGHT_0=49,26 PRIMER_RIGHT_0_TM=74.643 PRIMER_RIGHT_0_GC_PERCENT=57.692 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=22.0000 = SEQUENCE_ID=template_mis_16 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PICK_ANYWAY=1 PRIMER_MAX_POLY_X=40 PRIMER_MAX_SELF_END=100 PRIMER_WT_SIZE_GT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_TM_GT=.05 PRIMER_WT_TM_LT=.05 PRIMER_MAX_GC=100 PRIMER_MAX_TM=100 PRIMER_TASK=pick_right_only PRIMER_MAX_TEMPLATE_MISPRIMING=100 SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc SEQUENCE_INCLUDED_REGION=24,26 SEQUENCE_TEMPLATE=GCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAG P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_13 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.732142 PRIMER_RIGHT_0_SEQUENCE=CTTGATGGAGAATTGCGCAGAGGGGC PRIMER_RIGHT_0=49,26 PRIMER_RIGHT_0_TM=74.643 PRIMER_RIGHT_0_GC_PERCENT=57.692 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=22.0000 = SEQUENCE_ID=short_align_target_1 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_WT_SELF_ANY=0.0 PRIMER_WT_SELF_END=0.0 PRIMER_WT_NUM_NS=0.0 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_WT_END_QUAL=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_INTERNAL_MISHYB_LIBRARY= PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_INTERNAL_SALT_DIVALENT=0.0 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_INTERNAL_WT_TM_LT=1.0 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_TASK=pick_right_only PRIMER_FIRST_BASE_INDEX=1 PRIMER_PICK_ANYWAY=1 P3_COMMENT=Test for very short target of alignment number 1 PRIMER_ERROR=Missing SEQUENCE tag = SEQUENCE_ID=short_align_target_2 PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY= SEQUENCE_TEMPLATE=ACCAAGAAAGTAGTTGACAAAAAAATGAAAACCACTATTATTTAAATTACGGAAAAAAAAAAACCAATAAACAAAAAACATAATAAAAGAAATGAAAAAAAAGAATATTAGTAGAGCATACTGAATTGTTTTCAGAAAGAGAGTGAAAGGTAACAAAAATTTACCTACTTTTCCTTTTTATTTCTCTGGTTTCGTTTCTTTTTTTTCGGCCACCCTATTTATTTTTTCACTGCTTGTAAACAACGTCTCACTTCAATAGTGTCTCCTTCATGTACTGAGATACTGCTGCAAGCGAAATAT PRIMER_PRODUCT_SIZE_RANGE=150-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=24.00 PRIMER_MAX_TEMPLATE_MISPRIMING=12.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=24.00 PRIMER_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_MAX_SIZE=27 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_MAX_TM=63.0 PRIMER_PAIR_MAX_DIFF_TM=100.0 PRIMER_TM_FORMULA=0 PRIMER_MIN_GC=20.0 PRIMER_MAX_GC=80.0 PRIMER_MAX_SELF_ANY=8.00 PRIMER_MAX_SELF_END=3.00 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_POLY_X=5 PRIMER_OUTSIDE_PENALTY=0 PRIMER_GC_CLAMP=0 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_CORRECTIONS=0 PRIMER_SALT_DIVALENT=0.0 PRIMER_DNTP_CONC=0.0 PRIMER_DNA_CONC=50.0 PRIMER_LIBERAL_BASE=1 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_MIN_QUALITY=0 PRIMER_MIN_END_QUALITY=0 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_QUALITY_RANGE_MAX=100 PRIMER_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_WT_SELF_ANY=0.0 PRIMER_WT_SELF_END=0.0 PRIMER_WT_NUM_NS=0.0 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_WT_END_QUAL=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_INTERNAL_MISHYB_LIBRARY= PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_INTERNAL_SALT_DIVALENT=0.0 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_INTERNAL_WT_TM_LT=1.0 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_TASK=pick_left_only PRIMER_FIRST_BASE_INDEX=1 PRIMER_PICK_ANYWAY=1 P3_COMMENT=Test for very short target of alignment number 1 PRIMER_LEFT_EXPLAIN=considered 2141, GC content failed 553, low tm 1093, high tm 44, high any compl 1, high end compl 75, long poly-x seq 91, high 3' stability 6, high template mispriming score 9, ok 269 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.571405 PRIMER_LEFT_0_SEQUENCE=TTTTTCGGCCACCCTATTTA PRIMER_LEFT_0=202,20 PRIMER_LEFT_0_TM=59.429 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.2000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=6.0000 PRIMER_LEFT_1_PENALTY=1.175605 PRIMER_LEFT_1_SEQUENCE=AGATACTGCTGCAAGCGAAA PRIMER_LEFT_1=278,20 PRIMER_LEFT_1_TM=58.824 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.0000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING=7.0000 PRIMER_LEFT_2_PENALTY=1.299895 PRIMER_LEFT_2_SEQUENCE=TCGGCCACCCTATTTATTTTT PRIMER_LEFT_2=206,21 PRIMER_LEFT_2_TM=59.700 PRIMER_LEFT_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.6000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING=9.0000 PRIMER_LEFT_3_PENALTY=1.299895 PRIMER_LEFT_3_SEQUENCE=TTCGGCCACCCTATTTATTTT PRIMER_LEFT_3=205,21 PRIMER_LEFT_3_TM=59.700 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING=8.0000 PRIMER_LEFT_4_PENALTY=1.299895 PRIMER_LEFT_4_SEQUENCE=TTTCGGCCACCCTATTTATTT PRIMER_LEFT_4=204,21 PRIMER_LEFT_4_TM=59.700 PRIMER_LEFT_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.2000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING=9.0000 = primer3-2.3.6/test/th-w-other-tasks_output0000664000077300007730000144765112231156245017543 0ustar ioanaioanaP3_SETTINGS_FILE_USED=../th-w-other-tasks-settings.txt P3_FILE_TYPE=settings PRIMER_FIRST_BASE_INDEX=1 PRIMER_TASK=pick_pcr_primers P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY=../../src/humrep_and_simple.txt PRIMER_PRODUCT_SIZE_RANGE=300-400 401-500 200-299 501-600 100-199 601-700 701-850 851-1001 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00 PRIMER_MAX_SELF_ANY_TH=47.00 PRIMER_MAX_SELF_END_TH=47.00 PRIMER_MAX_HAIRPIN_TH=47.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.00 PRIMER_PAIR_MAX_COMPL_END_TH=47.00 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=80.00 PRIMER_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_MAX_SIZE=23 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=59.0 PRIMER_MAX_TM=63.0 PRIMER_PAIR_MAX_DIFF_TM=5.0 PRIMER_TM_FORMULA=1 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 PRIMER_DNTP_CONC=0.6 PRIMER_DNA_CONC=50.0 PRIMER_THERMODYNAMIC_PARAMETERS_PATH=../primer3_config/ PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_LOWERCASE_MASKING=0 PRIMER_MIN_GC=20.0 PRIMER_MAX_GC=80.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_POLY_X=5 PRIMER_OUTSIDE_PENALTY=0 PRIMER_GC_CLAMP=0 PRIMER_LIBERAL_BASE=1 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_WT_SELF_END_TH=0.0 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_WT_NUM_NS=0.0 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_WT_END_QUAL=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_END_TH=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_PICK_ANYWAY=0 P3_SETTINGS_FILE_END= PRIMER_TASK=generic PRIMER_OPT_SIZE=20 PRIMER_MIN_SIZE=19 PRIMER_MAX_SIZE=21 PRIMER_NUM_RETURN=1 PRIMER_MAX_SELF_ANY_TH=47.00 PRIMER_MAX_SELF_END_TH=47.00 PRIMER_MAX_HAIRPIN_TH=47.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.00 PRIMER_PAIR_MAX_COMPL_END_TH=47.00 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=80.00 PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY=../../src/humrep_and_simple.txt SEQUENCE_ID=pick_detection_primers_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_LEFT_EXPLAIN=considered 45, too many Ns 3, low tm 28, high repeat similarity 1, ok 13 PRIMER_RIGHT_EXPLAIN=considered 90, low tm 36, high tm 12, ok 42 PRIMER_PAIR_EXPLAIN=considered 18, unacceptable product size 11, high mispriming library similarity 4, ok 3 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=1.608735 PRIMER_LEFT_0_PENALTY=1.001812 PRIMER_RIGHT_0_PENALTY=0.606923 PRIMER_LEFT_0_SEQUENCE=AGACATGACACTGACGATGC PRIMER_RIGHT_0_SEQUENCE=AGCTATCGATTTGGGTCGGG PRIMER_LEFT_0=10,20 PRIMER_RIGHT_0=97,20 PRIMER_LEFT_0_TM=57.998 PRIMER_RIGHT_0_TM=59.607 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=41.74 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_0_END_STABILITY=3.9100 PRIMER_RIGHT_0_END_STABILITY=5.1400 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=8.8342 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=7.7569 PRIMER_PAIR_0_COMPL_ANY_TH=6.54 PRIMER_PAIR_0_COMPL_END_TH=6.54 PRIMER_PAIR_0_PRODUCT_SIZE=88 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=16.59 = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_detection_primers_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_EXPLAIN=considered 184, too many Ns 5, GC content failed 1, low tm 140, long poly-x seq 16, ok 22 PRIMER_RIGHT_EXPLAIN=considered 216, low tm 167, high tm 13, ok 36 PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 5, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=2.181798 PRIMER_LEFT_0_PENALTY=1.695359 PRIMER_RIGHT_0_PENALTY=0.486439 PRIMER_LEFT_0_SEQUENCE=TGACCTGATAGAAACGCCAAC PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT PRIMER_LEFT_0=53,21 PRIMER_RIGHT_0=137,20 PRIMER_LEFT_0_TM=58.305 PRIMER_RIGHT_0_TM=58.514 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=3.7700 PRIMER_RIGHT_0_END_STABILITY=3.0100 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=6.6359 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0760 PRIMER_PAIR_0_COMPL_ANY_TH=0.37 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=85 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=15.71 = PRIMER_TASK=pick_left_only PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_left_only_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_LEFT_EXPLAIN=considered 175, too many Ns 19, overlap target 25, low tm 64, high tm 12, high repeat similarity 3, ok 52 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.527529 PRIMER_LEFT_0_SEQUENCE=TTAGTGGGCCATTCGATCCC PRIMER_LEFT_0=61,20 PRIMER_LEFT_0_TM=59.528 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=5.82 PRIMER_LEFT_0_SELF_END_TH=5.82 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=2.6459 = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_left_only_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.486439 PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG PRIMER_LEFT_0=118,20 PRIMER_LEFT_0_TM=58.514 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner PRIMER_LEFT_0_END_STABILITY=5.3400 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=8.2325 = PRIMER_TASK=pick_right_only PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_right_only_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_RIGHT_EXPLAIN=considered 174, too many Ns 21, overlap target 23, low tm 64, high tm 12, high hairpin stability 16, high repeat similarity 3, ok 35 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.527529 PRIMER_RIGHT_0_SEQUENCE=GGGATCGAATGGCCCACTAA PRIMER_RIGHT_0=80,20 PRIMER_RIGHT_0_TM=59.528 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_SELF_ANY_TH=10.16 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=32.36 PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_RIGHT_0_END_STABILITY=2.2400 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0266 = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_right_only_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.486439 PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT PRIMER_RIGHT_0=137,20 PRIMER_RIGHT_0_TM=58.514 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon PRIMER_RIGHT_0_END_STABILITY=3.0100 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0760 = PRIMER_TASK=pick_hyb_probe_only PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_hyb_probe_only_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, ok 197 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_INTERNAL_0_PENALTY=0.727038 PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC PRIMER_INTERNAL_0=66,20 PRIMER_INTERNAL_0_TM=59.273 PRIMER_INTERNAL_0_GC_PERCENT=70.000 PRIMER_INTERNAL_0_SELF_ANY_TH=2.18 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=41.60 = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_hyb_probe_only_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_INTERNAL_0_PENALTY=0.093814 PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA PRIMER_INTERNAL_0=125,20 PRIMER_INTERNAL_0_TM=59.906 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_SELF_ANY_TH=7.82 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=36.42 = PRIMER_TASK=pick_primer_list PRIMER_NUM_RETURN=10000 PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_primer_list_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, ok 197 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=197 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_INTERNAL_0_PENALTY=0.727038 PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC PRIMER_INTERNAL_0=66,20 PRIMER_INTERNAL_0_TM=59.273 PRIMER_INTERNAL_0_GC_PERCENT=70.000 PRIMER_INTERNAL_0_SELF_ANY_TH=2.18 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=41.60 PRIMER_INTERNAL_1_PENALTY=1.416589 PRIMER_INTERNAL_1_SEQUENCE=GTGGGCCATTCGATCCCGACC PRIMER_INTERNAL_1=64,21 PRIMER_INTERNAL_1_TM=59.583 PRIMER_INTERNAL_1_GC_PERCENT=66.667 PRIMER_INTERNAL_1_SELF_ANY_TH=2.53 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=41.60 PRIMER_INTERNAL_2_PENALTY=1.438741 PRIMER_INTERNAL_2_SEQUENCE=GGCCATTCGATCCCGACCCA PRIMER_INTERNAL_2=67,20 PRIMER_INTERNAL_2_TM=58.561 PRIMER_INTERNAL_2_GC_PERCENT=65.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=41.60 PRIMER_INTERNAL_3_PENALTY=1.438741 PRIMER_INTERNAL_3_SEQUENCE=TGGGCCATTCGATCCCGACC PRIMER_INTERNAL_3=65,20 PRIMER_INTERNAL_3_TM=58.561 PRIMER_INTERNAL_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_SELF_ANY_TH=4.28 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=41.60 PRIMER_INTERNAL_4_PENALTY=1.650306 PRIMER_INTERNAL_4_SEQUENCE=GGGCCATTCGATCCCGACCCA PRIMER_INTERNAL_4=66,21 PRIMER_INTERNAL_4_TM=60.650 PRIMER_INTERNAL_4_GC_PERCENT=66.667 PRIMER_INTERNAL_4_SELF_ANY_TH=2.16 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=41.60 PRIMER_INTERNAL_5_PENALTY=1.650306 PRIMER_INTERNAL_5_SEQUENCE=TGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_5=65,21 PRIMER_INTERNAL_5_TM=60.650 PRIMER_INTERNAL_5_GC_PERCENT=66.667 PRIMER_INTERNAL_5_SELF_ANY_TH=4.28 PRIMER_INTERNAL_5_SELF_END_TH=0.51 PRIMER_INTERNAL_5_HAIRPIN_TH=41.60 PRIMER_INTERNAL_6_PENALTY=2.095978 PRIMER_INTERNAL_6_SEQUENCE=GGCCATTCGATCCCGACCCAA PRIMER_INTERNAL_6=67,21 PRIMER_INTERNAL_6_TM=58.904 PRIMER_INTERNAL_6_GC_PERCENT=61.905 PRIMER_INTERNAL_6_SELF_ANY_TH=0.00 PRIMER_INTERNAL_6_SELF_END_TH=0.00 PRIMER_INTERNAL_6_HAIRPIN_TH=41.60 PRIMER_INTERNAL_7_PENALTY=2.360266 PRIMER_INTERNAL_7_SEQUENCE=AGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_7=63,21 PRIMER_INTERNAL_7_TM=58.640 PRIMER_INTERNAL_7_GC_PERCENT=61.905 PRIMER_INTERNAL_7_SELF_ANY_TH=3.57 PRIMER_INTERNAL_7_SELF_END_TH=3.02 PRIMER_INTERNAL_7_HAIRPIN_TH=41.60 PRIMER_INTERNAL_8_PENALTY=2.456420 PRIMER_INTERNAL_8_SEQUENCE=AGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_8=63,20 PRIMER_INTERNAL_8_TM=57.544 PRIMER_INTERNAL_8_GC_PERCENT=60.000 PRIMER_INTERNAL_8_SELF_ANY_TH=3.28 PRIMER_INTERNAL_8_SELF_END_TH=0.00 PRIMER_INTERNAL_8_HAIRPIN_TH=41.96 PRIMER_INTERNAL_9_PENALTY=2.511372 PRIMER_INTERNAL_9_SEQUENCE=GTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_9=64,20 PRIMER_INTERNAL_9_TM=57.489 PRIMER_INTERNAL_9_GC_PERCENT=65.000 PRIMER_INTERNAL_9_SELF_ANY_TH=0.97 PRIMER_INTERNAL_9_SELF_END_TH=0.00 PRIMER_INTERNAL_9_HAIRPIN_TH=41.60 PRIMER_INTERNAL_10_PENALTY=2.626619 PRIMER_INTERNAL_10_SEQUENCE=AGTGGGCCATTCGATCCCGACC PRIMER_INTERNAL_10=63,22 PRIMER_INTERNAL_10_TM=60.627 PRIMER_INTERNAL_10_GC_PERCENT=63.636 PRIMER_INTERNAL_10_SELF_ANY_TH=5.61 PRIMER_INTERNAL_10_SELF_END_TH=0.00 PRIMER_INTERNAL_10_HAIRPIN_TH=41.60 PRIMER_INTERNAL_11_PENALTY=2.783921 PRIMER_INTERNAL_11_SEQUENCE=GGCCATTCGATCCCGACCCAAA PRIMER_INTERNAL_11=67,22 PRIMER_INTERNAL_11_TM=59.216 PRIMER_INTERNAL_11_GC_PERCENT=59.091 PRIMER_INTERNAL_11_SELF_ANY_TH=0.00 PRIMER_INTERNAL_11_SELF_END_TH=0.00 PRIMER_INTERNAL_11_HAIRPIN_TH=41.60 PRIMER_INTERNAL_12_PENALTY=2.885177 PRIMER_INTERNAL_12_SEQUENCE=GGGCCATTCGATCCCGACCCAA PRIMER_INTERNAL_12=66,22 PRIMER_INTERNAL_12_TM=60.885 PRIMER_INTERNAL_12_GC_PERCENT=63.636 PRIMER_INTERNAL_12_SELF_ANY_TH=2.16 PRIMER_INTERNAL_12_SELF_END_TH=0.00 PRIMER_INTERNAL_12_HAIRPIN_TH=41.60 PRIMER_INTERNAL_13_PENALTY=3.114431 PRIMER_INTERNAL_13_SEQUENCE=TAGTGGGCCATTCGATCCCGACC PRIMER_INTERNAL_13=62,23 PRIMER_INTERNAL_13_TM=60.114 PRIMER_INTERNAL_13_GC_PERCENT=60.870 PRIMER_INTERNAL_13_SELF_ANY_TH=5.61 PRIMER_INTERNAL_13_SELF_END_TH=0.00 PRIMER_INTERNAL_13_HAIRPIN_TH=41.60 PRIMER_INTERNAL_14_PENALTY=3.510595 PRIMER_INTERNAL_14_SEQUENCE=GTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_14=64,22 PRIMER_INTERNAL_14_TM=61.511 PRIMER_INTERNAL_14_GC_PERCENT=68.182 PRIMER_INTERNAL_14_SELF_ANY_TH=2.53 PRIMER_INTERNAL_14_SELF_END_TH=0.25 PRIMER_INTERNAL_14_HAIRPIN_TH=41.60 PRIMER_INTERNAL_15_PENALTY=3.685489 PRIMER_INTERNAL_15_SEQUENCE=GGCCATTCGATCCCGACCCAAAT PRIMER_INTERNAL_15=67,23 PRIMER_INTERNAL_15_TM=59.315 PRIMER_INTERNAL_15_GC_PERCENT=56.522 PRIMER_INTERNAL_15_SELF_ANY_TH=0.00 PRIMER_INTERNAL_15_SELF_END_TH=0.00 PRIMER_INTERNAL_15_HAIRPIN_TH=41.60 PRIMER_INTERNAL_16_PENALTY=3.807618 PRIMER_INTERNAL_16_SEQUENCE=TAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_16=62,22 PRIMER_INTERNAL_16_TM=58.192 PRIMER_INTERNAL_16_GC_PERCENT=59.091 PRIMER_INTERNAL_16_SELF_ANY_TH=3.57 PRIMER_INTERNAL_16_SELF_END_TH=3.02 PRIMER_INTERNAL_16_HAIRPIN_TH=41.60 PRIMER_INTERNAL_17_PENALTY=3.826170 PRIMER_INTERNAL_17_SEQUENCE=GCCATTCGATCCCGACCCAAA PRIMER_INTERNAL_17=68,21 PRIMER_INTERNAL_17_TM=57.174 PRIMER_INTERNAL_17_GC_PERCENT=57.143 PRIMER_INTERNAL_17_SELF_ANY_TH=0.00 PRIMER_INTERNAL_17_SELF_END_TH=0.00 PRIMER_INTERNAL_17_HAIRPIN_TH=41.60 PRIMER_INTERNAL_18_PENALTY=3.864865 PRIMER_INTERNAL_18_SEQUENCE=ACACACACACACAGCACACAGGT PRIMER_INTERNAL_18=37,23 PRIMER_INTERNAL_18_TM=59.135 PRIMER_INTERNAL_18_GC_PERCENT=52.174 PRIMER_INTERNAL_18_SELF_ANY_TH=0.00 PRIMER_INTERNAL_18_SELF_END_TH=0.00 PRIMER_INTERNAL_18_HAIRPIN_TH=0.00 PRIMER_INTERNAL_19_PENALTY=3.881423 PRIMER_INTERNAL_19_SEQUENCE=TAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_19=62,21 PRIMER_INTERNAL_19_TM=57.119 PRIMER_INTERNAL_19_GC_PERCENT=57.143 PRIMER_INTERNAL_19_SELF_ANY_TH=0.00 PRIMER_INTERNAL_19_SELF_END_TH=0.00 PRIMER_INTERNAL_19_HAIRPIN_TH=41.96 PRIMER_INTERNAL_20_PENALTY=3.936624 PRIMER_INTERNAL_20_SEQUENCE=TGGGCCATTCGATCCCGACCCA PRIMER_INTERNAL_20=65,22 PRIMER_INTERNAL_20_TM=61.937 PRIMER_INTERNAL_20_GC_PERCENT=63.636 PRIMER_INTERNAL_20_SELF_ANY_TH=8.58 PRIMER_INTERNAL_20_SELF_END_TH=6.38 PRIMER_INTERNAL_20_HAIRPIN_TH=42.67 PRIMER_INTERNAL_21_PENALTY=3.956741 PRIMER_INTERNAL_21_SEQUENCE=GGCCATTCGATCCCGACCC PRIMER_INTERNAL_21=67,19 PRIMER_INTERNAL_21_TM=57.043 PRIMER_INTERNAL_21_GC_PERCENT=68.421 PRIMER_INTERNAL_21_SELF_ANY_TH=0.00 PRIMER_INTERNAL_21_SELF_END_TH=0.00 PRIMER_INTERNAL_21_HAIRPIN_TH=41.60 PRIMER_INTERNAL_22_PENALTY=3.956741 PRIMER_INTERNAL_22_SEQUENCE=GGGCCATTCGATCCCGACC PRIMER_INTERNAL_22=66,19 PRIMER_INTERNAL_22_TM=57.043 PRIMER_INTERNAL_22_GC_PERCENT=68.421 PRIMER_INTERNAL_22_SELF_ANY_TH=2.18 PRIMER_INTERNAL_22_SELF_END_TH=0.00 PRIMER_INTERNAL_22_HAIRPIN_TH=41.60 PRIMER_INTERNAL_23_PENALTY=4.001118 PRIMER_INTERNAL_23_SEQUENCE=CACACACACACACAGCACACAGGT PRIMER_INTERNAL_23=36,24 PRIMER_INTERNAL_23_TM=60.001 PRIMER_INTERNAL_23_GC_PERCENT=54.167 PRIMER_INTERNAL_23_SELF_ANY_TH=0.00 PRIMER_INTERNAL_23_SELF_END_TH=0.00 PRIMER_INTERNAL_23_HAIRPIN_TH=0.00 PRIMER_INTERNAL_24_PENALTY=4.001118 PRIMER_INTERNAL_24_SEQUENCE=ACACACACACACACAGCACACAGG PRIMER_INTERNAL_24=35,24 PRIMER_INTERNAL_24_TM=60.001 PRIMER_INTERNAL_24_GC_PERCENT=54.167 PRIMER_INTERNAL_24_SELF_ANY_TH=0.00 PRIMER_INTERNAL_24_SELF_END_TH=0.00 PRIMER_INTERNAL_24_HAIRPIN_TH=0.00 PRIMER_INTERNAL_25_PENALTY=4.058186 PRIMER_INTERNAL_25_SEQUENCE=GGCCATTCGATCCCGACCCAAATC PRIMER_INTERNAL_25=67,24 PRIMER_INTERNAL_25_TM=59.942 PRIMER_INTERNAL_25_GC_PERCENT=58.333 PRIMER_INTERNAL_25_SELF_ANY_TH=0.00 PRIMER_INTERNAL_25_SELF_END_TH=0.00 PRIMER_INTERNAL_25_HAIRPIN_TH=41.60 PRIMER_INTERNAL_26_PENALTY=4.099823 PRIMER_INTERNAL_26_SEQUENCE=GGGCCATTCGATCCCGACCCAAA PRIMER_INTERNAL_26=66,23 PRIMER_INTERNAL_26_TM=61.100 PRIMER_INTERNAL_26_GC_PERCENT=60.870 PRIMER_INTERNAL_26_SELF_ANY_TH=2.16 PRIMER_INTERNAL_26_SELF_END_TH=0.00 PRIMER_INTERNAL_26_HAIRPIN_TH=41.60 PRIMER_INTERNAL_27_PENALTY=4.159384 PRIMER_INTERNAL_27_SEQUENCE=CACACACACACAGCACACAGGT PRIMER_INTERNAL_27=38,22 PRIMER_INTERNAL_27_TM=57.841 PRIMER_INTERNAL_27_GC_PERCENT=54.545 PRIMER_INTERNAL_27_SELF_ANY_TH=0.00 PRIMER_INTERNAL_27_SELF_END_TH=0.00 PRIMER_INTERNAL_27_HAIRPIN_TH=0.00 PRIMER_INTERNAL_28_PENALTY=4.159384 PRIMER_INTERNAL_28_SEQUENCE=ACACACACACACAGCACACAGG PRIMER_INTERNAL_28=37,22 PRIMER_INTERNAL_28_TM=57.841 PRIMER_INTERNAL_28_GC_PERCENT=54.545 PRIMER_INTERNAL_28_SELF_ANY_TH=0.00 PRIMER_INTERNAL_28_SELF_END_TH=0.00 PRIMER_INTERNAL_28_HAIRPIN_TH=0.00 PRIMER_INTERNAL_29_PENALTY=4.196497 PRIMER_INTERNAL_29_SEQUENCE=CACACACACACACAGCACACAGG PRIMER_INTERNAL_29=36,23 PRIMER_INTERNAL_29_TM=58.804 PRIMER_INTERNAL_29_GC_PERCENT=56.522 PRIMER_INTERNAL_29_SELF_ANY_TH=0.00 PRIMER_INTERNAL_29_SELF_END_TH=0.00 PRIMER_INTERNAL_29_HAIRPIN_TH=0.00 PRIMER_INTERNAL_30_PENALTY=4.232604 PRIMER_INTERNAL_30_SEQUENCE=GCCATTCGATCCCGACCCAAATCG PRIMER_INTERNAL_30=68,24 PRIMER_INTERNAL_30_TM=60.233 PRIMER_INTERNAL_30_GC_PERCENT=58.333 PRIMER_INTERNAL_30_SELF_ANY_TH=0.00 PRIMER_INTERNAL_30_SELF_END_TH=0.00 PRIMER_INTERNAL_30_HAIRPIN_TH=41.60 PRIMER_INTERNAL_31_PENALTY=4.351864 PRIMER_INTERNAL_31_SEQUENCE=TTAGTGGGCCATTCGATCCCGACC PRIMER_INTERNAL_31=61,24 PRIMER_INTERNAL_31_TM=60.352 PRIMER_INTERNAL_31_GC_PERCENT=58.333 PRIMER_INTERNAL_31_SELF_ANY_TH=5.61 PRIMER_INTERNAL_31_SELF_END_TH=0.00 PRIMER_INTERNAL_31_HAIRPIN_TH=41.60 PRIMER_INTERNAL_32_PENALTY=4.478673 PRIMER_INTERNAL_32_SEQUENCE=TTAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_32=61,23 PRIMER_INTERNAL_32_TM=58.521 PRIMER_INTERNAL_32_GC_PERCENT=56.522 PRIMER_INTERNAL_32_SELF_ANY_TH=3.57 PRIMER_INTERNAL_32_SELF_END_TH=3.02 PRIMER_INTERNAL_32_HAIRPIN_TH=41.60 PRIMER_INTERNAL_33_PENALTY=4.484274 PRIMER_INTERNAL_33_SEQUENCE=TTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_33=61,22 PRIMER_INTERNAL_33_TM=57.516 PRIMER_INTERNAL_33_GC_PERCENT=54.545 PRIMER_INTERNAL_33_SELF_ANY_TH=0.00 PRIMER_INTERNAL_33_SELF_END_TH=0.00 PRIMER_INTERNAL_33_HAIRPIN_TH=41.96 PRIMER_INTERNAL_34_PENALTY=4.552303 PRIMER_INTERNAL_34_SEQUENCE=ACACACACACACACAGCACACA PRIMER_INTERNAL_34=35,22 PRIMER_INTERNAL_34_TM=57.448 PRIMER_INTERNAL_34_GC_PERCENT=50.000 PRIMER_INTERNAL_34_SELF_ANY_TH=0.00 PRIMER_INTERNAL_34_SELF_END_TH=0.00 PRIMER_INTERNAL_34_HAIRPIN_TH=0.00 PRIMER_INTERNAL_35_PENALTY=4.639434 PRIMER_INTERNAL_35_SEQUENCE=GCCATTCGATCCCGACCCAAAT PRIMER_INTERNAL_35=68,22 PRIMER_INTERNAL_35_TM=57.361 PRIMER_INTERNAL_35_GC_PERCENT=54.545 PRIMER_INTERNAL_35_SELF_ANY_TH=0.00 PRIMER_INTERNAL_35_SELF_END_TH=0.00 PRIMER_INTERNAL_35_HAIRPIN_TH=41.60 PRIMER_INTERNAL_36_PENALTY=4.809017 PRIMER_INTERNAL_36_SEQUENCE=ACACACACACACACAGCACACAG PRIMER_INTERNAL_36=35,23 PRIMER_INTERNAL_36_TM=58.191 PRIMER_INTERNAL_36_GC_PERCENT=52.174 PRIMER_INTERNAL_36_SELF_ANY_TH=0.00 PRIMER_INTERNAL_36_SELF_END_TH=0.00 PRIMER_INTERNAL_36_HAIRPIN_TH=0.00 PRIMER_INTERNAL_37_PENALTY=4.881095 PRIMER_INTERNAL_37_SEQUENCE=GCCATTCGATCCCGACCCAAATC PRIMER_INTERNAL_37=68,23 PRIMER_INTERNAL_37_TM=58.119 PRIMER_INTERNAL_37_GC_PERCENT=56.522 PRIMER_INTERNAL_37_SELF_ANY_TH=0.00 PRIMER_INTERNAL_37_SELF_END_TH=0.00 PRIMER_INTERNAL_37_HAIRPIN_TH=41.60 PRIMER_INTERNAL_38_PENALTY=5.108025 PRIMER_INTERNAL_38_SEQUENCE=TGGGCCATTCGATCCCGACCCAA PRIMER_INTERNAL_38=65,23 PRIMER_INTERNAL_38_TM=62.108 PRIMER_INTERNAL_38_GC_PERCENT=60.870 PRIMER_INTERNAL_38_SELF_ANY_TH=8.58 PRIMER_INTERNAL_38_SELF_END_TH=0.00 PRIMER_INTERNAL_38_HAIRPIN_TH=41.29 PRIMER_INTERNAL_39_PENALTY=5.111368 PRIMER_INTERNAL_39_SEQUENCE=CCATTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_39=69,24 PRIMER_INTERNAL_39_TM=58.889 PRIMER_INTERNAL_39_GC_PERCENT=54.167 PRIMER_INTERNAL_39_SELF_ANY_TH=0.00 PRIMER_INTERNAL_39_SELF_END_TH=0.00 PRIMER_INTERNAL_39_HAIRPIN_TH=41.60 PRIMER_INTERNAL_40_PENALTY=5.111455 PRIMER_INTERNAL_40_SEQUENCE=CCATTCGATCCCGACCCAAATCG PRIMER_INTERNAL_40=69,23 PRIMER_INTERNAL_40_TM=57.889 PRIMER_INTERNAL_40_GC_PERCENT=56.522 PRIMER_INTERNAL_40_SELF_ANY_TH=0.00 PRIMER_INTERNAL_40_SELF_END_TH=0.00 PRIMER_INTERNAL_40_HAIRPIN_TH=41.60 PRIMER_INTERNAL_41_PENALTY=5.114088 PRIMER_INTERNAL_41_SEQUENCE=ACAGCACACAGGTATTAGTGGGCCA PRIMER_INTERNAL_41=47,25 PRIMER_INTERNAL_41_TM=59.886 PRIMER_INTERNAL_41_GC_PERCENT=52.000 PRIMER_INTERNAL_41_SELF_ANY_TH=8.63 PRIMER_INTERNAL_41_SELF_END_TH=1.50 PRIMER_INTERNAL_41_HAIRPIN_TH=32.68 PRIMER_INTERNAL_42_PENALTY=5.122599 PRIMER_INTERNAL_42_SEQUENCE=GGGCCATTCGATCCCGACCCAAAT PRIMER_INTERNAL_42=66,24 PRIMER_INTERNAL_42_TM=61.123 PRIMER_INTERNAL_42_GC_PERCENT=58.333 PRIMER_INTERNAL_42_SELF_ANY_TH=2.16 PRIMER_INTERNAL_42_SELF_END_TH=0.00 PRIMER_INTERNAL_42_HAIRPIN_TH=41.60 PRIMER_INTERNAL_43_PENALTY=5.268172 PRIMER_INTERNAL_43_SEQUENCE=AGCACACAGGTATTAGTGGGCCA PRIMER_INTERNAL_43=49,23 PRIMER_INTERNAL_43_TM=57.732 PRIMER_INTERNAL_43_GC_PERCENT=52.174 PRIMER_INTERNAL_43_SELF_ANY_TH=8.63 PRIMER_INTERNAL_43_SELF_END_TH=1.50 PRIMER_INTERNAL_43_HAIRPIN_TH=32.68 PRIMER_INTERNAL_44_PENALTY=5.295331 PRIMER_INTERNAL_44_SEQUENCE=ACACACACACACAGCACACAGGTA PRIMER_INTERNAL_44=37,24 PRIMER_INTERNAL_44_TM=58.705 PRIMER_INTERNAL_44_GC_PERCENT=50.000 PRIMER_INTERNAL_44_SELF_ANY_TH=0.00 PRIMER_INTERNAL_44_SELF_END_TH=0.00 PRIMER_INTERNAL_44_HAIRPIN_TH=0.00 PRIMER_INTERNAL_45_PENALTY=5.306369 PRIMER_INTERNAL_45_SEQUENCE=CAGCACACAGGTATTAGTGGGCCA PRIMER_INTERNAL_45=48,24 PRIMER_INTERNAL_45_TM=58.694 PRIMER_INTERNAL_45_GC_PERCENT=54.167 PRIMER_INTERNAL_45_SELF_ANY_TH=8.63 PRIMER_INTERNAL_45_SELF_END_TH=1.50 PRIMER_INTERNAL_45_HAIRPIN_TH=32.68 PRIMER_INTERNAL_46_PENALTY=5.307164 PRIMER_INTERNAL_46_SEQUENCE=ACAGCACACAGGTATTAGTGGGCC PRIMER_INTERNAL_46=47,24 PRIMER_INTERNAL_46_TM=58.693 PRIMER_INTERNAL_46_GC_PERCENT=54.167 PRIMER_INTERNAL_46_SELF_ANY_TH=8.63 PRIMER_INTERNAL_46_SELF_END_TH=0.00 PRIMER_INTERNAL_46_HAIRPIN_TH=32.68 PRIMER_INTERNAL_47_PENALTY=5.316271 PRIMER_INTERNAL_47_SEQUENCE=ATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_47=60,23 PRIMER_INTERNAL_47_TM=57.684 PRIMER_INTERNAL_47_GC_PERCENT=52.174 PRIMER_INTERNAL_47_SELF_ANY_TH=0.00 PRIMER_INTERNAL_47_SELF_END_TH=0.00 PRIMER_INTERNAL_47_HAIRPIN_TH=41.96 PRIMER_INTERNAL_48_PENALTY=5.357946 PRIMER_INTERNAL_48_SEQUENCE=ATTAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_48=60,24 PRIMER_INTERNAL_48_TM=58.642 PRIMER_INTERNAL_48_GC_PERCENT=54.167 PRIMER_INTERNAL_48_SELF_ANY_TH=3.57 PRIMER_INTERNAL_48_SELF_END_TH=3.02 PRIMER_INTERNAL_48_HAIRPIN_TH=41.60 PRIMER_INTERNAL_49_PENALTY=5.401146 PRIMER_INTERNAL_49_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACC PRIMER_INTERNAL_49=60,25 PRIMER_INTERNAL_49_TM=60.401 PRIMER_INTERNAL_49_GC_PERCENT=56.000 PRIMER_INTERNAL_49_SELF_ANY_TH=5.61 PRIMER_INTERNAL_49_SELF_END_TH=0.00 PRIMER_INTERNAL_49_HAIRPIN_TH=41.60 PRIMER_INTERNAL_50_PENALTY=5.439697 PRIMER_INTERNAL_50_SEQUENCE=CACACACACACACAGCACACAGGTA PRIMER_INTERNAL_50=36,25 PRIMER_INTERNAL_50_TM=59.560 PRIMER_INTERNAL_50_GC_PERCENT=52.000 PRIMER_INTERNAL_50_SELF_ANY_TH=0.00 PRIMER_INTERNAL_50_SELF_END_TH=0.00 PRIMER_INTERNAL_50_HAIRPIN_TH=0.00 PRIMER_INTERNAL_51_PENALTY=5.444425 PRIMER_INTERNAL_51_SEQUENCE=CACAGCACACAGGTATTAGTGGGCC PRIMER_INTERNAL_51=46,25 PRIMER_INTERNAL_51_TM=59.556 PRIMER_INTERNAL_51_GC_PERCENT=56.000 PRIMER_INTERNAL_51_SELF_ANY_TH=8.63 PRIMER_INTERNAL_51_SELF_END_TH=0.00 PRIMER_INTERNAL_51_HAIRPIN_TH=32.68 PRIMER_INTERNAL_52_PENALTY=5.459011 PRIMER_INTERNAL_52_SEQUENCE=AGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_52=63,23 PRIMER_INTERNAL_52_TM=62.459 PRIMER_INTERNAL_52_GC_PERCENT=65.217 PRIMER_INTERNAL_52_SELF_ANY_TH=5.61 PRIMER_INTERNAL_52_SELF_END_TH=2.93 PRIMER_INTERNAL_52_HAIRPIN_TH=41.60 PRIMER_INTERNAL_53_PENALTY=5.514983 PRIMER_INTERNAL_53_SEQUENCE=TCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_53=73,24 PRIMER_INTERNAL_53_TM=58.485 PRIMER_INTERNAL_53_GC_PERCENT=54.167 PRIMER_INTERNAL_53_SELF_ANY_TH=0.30 PRIMER_INTERNAL_53_SELF_END_TH=0.00 PRIMER_INTERNAL_53_HAIRPIN_TH=41.28 PRIMER_INTERNAL_54_PENALTY=5.518553 PRIMER_INTERNAL_54_SEQUENCE=CGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_54=74,24 PRIMER_INTERNAL_54_TM=58.481 PRIMER_INTERNAL_54_GC_PERCENT=54.167 PRIMER_INTERNAL_54_SELF_ANY_TH=0.00 PRIMER_INTERNAL_54_SELF_END_TH=0.00 PRIMER_INTERNAL_54_HAIRPIN_TH=41.28 PRIMER_INTERNAL_55_PENALTY=5.523565 PRIMER_INTERNAL_55_SEQUENCE=CGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_55=74,23 PRIMER_INTERNAL_55_TM=57.476 PRIMER_INTERNAL_55_GC_PERCENT=56.522 PRIMER_INTERNAL_55_SELF_ANY_TH=0.00 PRIMER_INTERNAL_55_SELF_END_TH=0.00 PRIMER_INTERNAL_55_HAIRPIN_TH=41.28 PRIMER_INTERNAL_56_PENALTY=5.550131 PRIMER_INTERNAL_56_SEQUENCE=CACACACACACAGCACACAGGTA PRIMER_INTERNAL_56=38,23 PRIMER_INTERNAL_56_TM=57.450 PRIMER_INTERNAL_56_GC_PERCENT=52.174 PRIMER_INTERNAL_56_SELF_ANY_TH=0.00 PRIMER_INTERNAL_56_SELF_END_TH=0.00 PRIMER_INTERNAL_56_HAIRPIN_TH=0.00 PRIMER_INTERNAL_57_PENALTY=5.564155 PRIMER_INTERNAL_57_SEQUENCE=TCGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_57=73,25 PRIMER_INTERNAL_57_TM=59.436 PRIMER_INTERNAL_57_GC_PERCENT=52.000 PRIMER_INTERNAL_57_SELF_ANY_TH=0.30 PRIMER_INTERNAL_57_SELF_END_TH=0.00 PRIMER_INTERNAL_57_HAIRPIN_TH=41.28 PRIMER_INTERNAL_58_PENALTY=5.572672 PRIMER_INTERNAL_58_SEQUENCE=CAGCACACAGGTATTAGTGGGCC PRIMER_INTERNAL_58=48,23 PRIMER_INTERNAL_58_TM=57.427 PRIMER_INTERNAL_58_GC_PERCENT=56.522 PRIMER_INTERNAL_58_SELF_ANY_TH=8.63 PRIMER_INTERNAL_58_SELF_END_TH=0.00 PRIMER_INTERNAL_58_HAIRPIN_TH=32.68 PRIMER_INTERNAL_59_PENALTY=5.697915 PRIMER_INTERNAL_59_SEQUENCE=GTGGGCCATTCGATCCCGACCCA PRIMER_INTERNAL_59=64,23 PRIMER_INTERNAL_59_TM=62.698 PRIMER_INTERNAL_59_GC_PERCENT=65.217 PRIMER_INTERNAL_59_SELF_ANY_TH=8.58 PRIMER_INTERNAL_59_SELF_END_TH=6.40 PRIMER_INTERNAL_59_HAIRPIN_TH=42.67 PRIMER_INTERNAL_60_PENALTY=5.806699 PRIMER_INTERNAL_60_SEQUENCE=CCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_60=78,24 PRIMER_INTERNAL_60_TM=58.193 PRIMER_INTERNAL_60_GC_PERCENT=54.167 PRIMER_INTERNAL_60_SELF_ANY_TH=12.68 PRIMER_INTERNAL_60_SELF_END_TH=12.68 PRIMER_INTERNAL_60_HAIRPIN_TH=41.28 PRIMER_INTERNAL_61_PENALTY=5.806699 PRIMER_INTERNAL_61_SEQUENCE=TCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_61=77,24 PRIMER_INTERNAL_61_TM=58.193 PRIMER_INTERNAL_61_GC_PERCENT=54.167 PRIMER_INTERNAL_61_SELF_ANY_TH=5.17 PRIMER_INTERNAL_61_SELF_END_TH=5.17 PRIMER_INTERNAL_61_HAIRPIN_TH=41.28 PRIMER_INTERNAL_62_PENALTY=5.824076 PRIMER_INTERNAL_62_SEQUENCE=CCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_62=78,23 PRIMER_INTERNAL_62_TM=57.176 PRIMER_INTERNAL_62_GC_PERCENT=56.522 PRIMER_INTERNAL_62_SELF_ANY_TH=5.17 PRIMER_INTERNAL_62_SELF_END_TH=5.17 PRIMER_INTERNAL_62_HAIRPIN_TH=41.28 PRIMER_INTERNAL_63_PENALTY=5.846052 PRIMER_INTERNAL_63_SEQUENCE=TCCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_63=77,25 PRIMER_INTERNAL_63_TM=59.154 PRIMER_INTERNAL_63_GC_PERCENT=52.000 PRIMER_INTERNAL_63_SELF_ANY_TH=12.68 PRIMER_INTERNAL_63_SELF_END_TH=12.68 PRIMER_INTERNAL_63_HAIRPIN_TH=41.28 PRIMER_INTERNAL_64_PENALTY=5.892980 PRIMER_INTERNAL_64_SEQUENCE=TAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_64=62,24 PRIMER_INTERNAL_64_TM=61.893 PRIMER_INTERNAL_64_GC_PERCENT=62.500 PRIMER_INTERNAL_64_SELF_ANY_TH=5.61 PRIMER_INTERNAL_64_SELF_END_TH=2.93 PRIMER_INTERNAL_64_HAIRPIN_TH=41.60 PRIMER_INTERNAL_65_PENALTY=5.951461 PRIMER_INTERNAL_65_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_65=57,25 PRIMER_INTERNAL_65_TM=59.049 PRIMER_INTERNAL_65_GC_PERCENT=56.000 PRIMER_INTERNAL_65_SELF_ANY_TH=4.28 PRIMER_INTERNAL_65_SELF_END_TH=0.00 PRIMER_INTERNAL_65_HAIRPIN_TH=37.07 PRIMER_INTERNAL_66_PENALTY=5.980536 PRIMER_INTERNAL_66_SEQUENCE=CATTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_66=70,23 PRIMER_INTERNAL_66_TM=57.019 PRIMER_INTERNAL_66_GC_PERCENT=52.174 PRIMER_INTERNAL_66_SELF_ANY_TH=0.00 PRIMER_INTERNAL_66_SELF_END_TH=0.00 PRIMER_INTERNAL_66_HAIRPIN_TH=41.60 PRIMER_INTERNAL_67_PENALTY=6.009338 PRIMER_INTERNAL_67_SEQUENCE=CCATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_67=69,25 PRIMER_INTERNAL_67_TM=58.991 PRIMER_INTERNAL_67_GC_PERCENT=52.000 PRIMER_INTERNAL_67_SELF_ANY_TH=0.00 PRIMER_INTERNAL_67_SELF_END_TH=0.00 PRIMER_INTERNAL_67_HAIRPIN_TH=41.60 PRIMER_INTERNAL_68_PENALTY=6.015417 PRIMER_INTERNAL_68_SEQUENCE=ACACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_68=45,25 PRIMER_INTERNAL_68_TM=58.985 PRIMER_INTERNAL_68_GC_PERCENT=52.000 PRIMER_INTERNAL_68_SELF_ANY_TH=5.21 PRIMER_INTERNAL_68_SELF_END_TH=5.21 PRIMER_INTERNAL_68_HAIRPIN_TH=33.51 PRIMER_INTERNAL_69_PENALTY=6.046206 PRIMER_INTERNAL_69_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACC PRIMER_INTERNAL_69=59,26 PRIMER_INTERNAL_69_TM=59.954 PRIMER_INTERNAL_69_GC_PERCENT=53.846 PRIMER_INTERNAL_69_SELF_ANY_TH=5.61 PRIMER_INTERNAL_69_SELF_END_TH=0.00 PRIMER_INTERNAL_69_HAIRPIN_TH=41.60 PRIMER_INTERNAL_70_PENALTY=6.046206 PRIMER_INTERNAL_70_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_70=57,26 PRIMER_INTERNAL_70_TM=59.954 PRIMER_INTERNAL_70_GC_PERCENT=53.846 PRIMER_INTERNAL_70_SELF_ANY_TH=0.76 PRIMER_INTERNAL_70_SELF_END_TH=0.00 PRIMER_INTERNAL_70_HAIRPIN_TH=41.96 PRIMER_INTERNAL_71_PENALTY=6.046659 PRIMER_INTERNAL_71_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_71=56,26 PRIMER_INTERNAL_71_TM=59.953 PRIMER_INTERNAL_71_GC_PERCENT=53.846 PRIMER_INTERNAL_71_SELF_ANY_TH=4.31 PRIMER_INTERNAL_71_SELF_END_TH=0.00 PRIMER_INTERNAL_71_HAIRPIN_TH=37.07 PRIMER_INTERNAL_72_PENALTY=6.049301 PRIMER_INTERNAL_72_SEQUENCE=ACAGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_72=47,26 PRIMER_INTERNAL_72_TM=59.951 PRIMER_INTERNAL_72_GC_PERCENT=50.000 PRIMER_INTERNAL_72_SELF_ANY_TH=8.63 PRIMER_INTERNAL_72_SELF_END_TH=0.00 PRIMER_INTERNAL_72_HAIRPIN_TH=32.68 PRIMER_INTERNAL_73_PENALTY=6.095821 PRIMER_INTERNAL_73_SEQUENCE=GCACACAGGTATTAGTGGGCCATTCG PRIMER_INTERNAL_73=50,26 PRIMER_INTERNAL_73_TM=59.904 PRIMER_INTERNAL_73_GC_PERCENT=53.846 PRIMER_INTERNAL_73_SELF_ANY_TH=2.67 PRIMER_INTERNAL_73_SELF_END_TH=0.00 PRIMER_INTERNAL_73_HAIRPIN_TH=28.81 PRIMER_INTERNAL_74_PENALTY=6.114152 PRIMER_INTERNAL_74_SEQUENCE=AGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_74=49,24 PRIMER_INTERNAL_74_TM=57.886 PRIMER_INTERNAL_74_GC_PERCENT=50.000 PRIMER_INTERNAL_74_SELF_ANY_TH=8.63 PRIMER_INTERNAL_74_SELF_END_TH=0.00 PRIMER_INTERNAL_74_HAIRPIN_TH=32.68 PRIMER_INTERNAL_75_PENALTY=6.125318 PRIMER_INTERNAL_75_SEQUENCE=GCCATTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_75=68,25 PRIMER_INTERNAL_75_TM=61.125 PRIMER_INTERNAL_75_GC_PERCENT=56.000 PRIMER_INTERNAL_75_SELF_ANY_TH=0.00 PRIMER_INTERNAL_75_SELF_END_TH=0.00 PRIMER_INTERNAL_75_HAIRPIN_TH=41.60 PRIMER_INTERNAL_76_PENALTY=6.131527 PRIMER_INTERNAL_76_SEQUENCE=ACACACACACACACAGCACACAGGT PRIMER_INTERNAL_76=35,25 PRIMER_INTERNAL_76_TM=61.132 PRIMER_INTERNAL_76_GC_PERCENT=52.000 PRIMER_INTERNAL_76_SELF_ANY_TH=0.00 PRIMER_INTERNAL_76_SELF_END_TH=0.00 PRIMER_INTERNAL_76_HAIRPIN_TH=0.00 PRIMER_INTERNAL_77_PENALTY=6.186005 PRIMER_INTERNAL_77_SEQUENCE=ACACACACACACAGCACACAGGTAT PRIMER_INTERNAL_77=37,25 PRIMER_INTERNAL_77_TM=58.814 PRIMER_INTERNAL_77_GC_PERCENT=48.000 PRIMER_INTERNAL_77_SELF_ANY_TH=0.00 PRIMER_INTERNAL_77_SELF_END_TH=0.00 PRIMER_INTERNAL_77_HAIRPIN_TH=0.00 PRIMER_INTERNAL_78_PENALTY=6.195851 PRIMER_INTERNAL_78_SEQUENCE=CAGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_78=48,25 PRIMER_INTERNAL_78_TM=58.804 PRIMER_INTERNAL_78_GC_PERCENT=52.000 PRIMER_INTERNAL_78_SELF_ANY_TH=8.63 PRIMER_INTERNAL_78_SELF_END_TH=0.00 PRIMER_INTERNAL_78_HAIRPIN_TH=32.68 PRIMER_INTERNAL_79_PENALTY=6.203782 PRIMER_INTERNAL_79_SEQUENCE=CACACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_79=44,26 PRIMER_INTERNAL_79_TM=59.796 PRIMER_INTERNAL_79_GC_PERCENT=53.846 PRIMER_INTERNAL_79_SELF_ANY_TH=5.21 PRIMER_INTERNAL_79_SELF_END_TH=5.21 PRIMER_INTERNAL_79_HAIRPIN_TH=33.51 PRIMER_INTERNAL_80_PENALTY=6.214266 PRIMER_INTERNAL_80_SEQUENCE=CACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_80=46,24 PRIMER_INTERNAL_80_TM=57.786 PRIMER_INTERNAL_80_GC_PERCENT=54.167 PRIMER_INTERNAL_80_SELF_ANY_TH=5.21 PRIMER_INTERNAL_80_SELF_END_TH=5.21 PRIMER_INTERNAL_80_HAIRPIN_TH=33.51 PRIMER_INTERNAL_81_PENALTY=6.225896 PRIMER_INTERNAL_81_SEQUENCE=TTCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_81=72,25 PRIMER_INTERNAL_81_TM=58.774 PRIMER_INTERNAL_81_GC_PERCENT=52.000 PRIMER_INTERNAL_81_SELF_ANY_TH=0.00 PRIMER_INTERNAL_81_SELF_END_TH=0.00 PRIMER_INTERNAL_81_HAIRPIN_TH=41.60 PRIMER_INTERNAL_82_PENALTY=6.265114 PRIMER_INTERNAL_82_SEQUENCE=TGGGCCATTCGATCCCGACCCAAA PRIMER_INTERNAL_82=65,24 PRIMER_INTERNAL_82_TM=62.265 PRIMER_INTERNAL_82_GC_PERCENT=58.333 PRIMER_INTERNAL_82_SELF_ANY_TH=8.58 PRIMER_INTERNAL_82_SELF_END_TH=0.00 PRIMER_INTERNAL_82_HAIRPIN_TH=41.29 PRIMER_INTERNAL_83_PENALTY=6.318558 PRIMER_INTERNAL_83_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_83=72,26 PRIMER_INTERNAL_83_TM=59.681 PRIMER_INTERNAL_83_GC_PERCENT=50.000 PRIMER_INTERNAL_83_SELF_ANY_TH=0.00 PRIMER_INTERNAL_83_SELF_END_TH=0.00 PRIMER_INTERNAL_83_HAIRPIN_TH=41.60 PRIMER_INTERNAL_84_PENALTY=6.365515 PRIMER_INTERNAL_84_SEQUENCE=CACACACACACACAGCACACAGGTAT PRIMER_INTERNAL_84=36,26 PRIMER_INTERNAL_84_TM=59.634 PRIMER_INTERNAL_84_GC_PERCENT=50.000 PRIMER_INTERNAL_84_SELF_ANY_TH=0.00 PRIMER_INTERNAL_84_SELF_END_TH=0.00 PRIMER_INTERNAL_84_HAIRPIN_TH=0.00 PRIMER_INTERNAL_85_PENALTY=6.390437 PRIMER_INTERNAL_85_SEQUENCE=CACACACACACAGCACACAGGTAT PRIMER_INTERNAL_85=38,24 PRIMER_INTERNAL_85_TM=57.610 PRIMER_INTERNAL_85_GC_PERCENT=50.000 PRIMER_INTERNAL_85_SELF_ANY_TH=0.00 PRIMER_INTERNAL_85_SELF_END_TH=0.00 PRIMER_INTERNAL_85_HAIRPIN_TH=0.00 PRIMER_INTERNAL_86_PENALTY=6.635581 PRIMER_INTERNAL_86_SEQUENCE=GGGCCATTCGATCCCGACCCAAATC PRIMER_INTERNAL_86=66,25 PRIMER_INTERNAL_86_TM=61.636 PRIMER_INTERNAL_86_GC_PERCENT=60.000 PRIMER_INTERNAL_86_SELF_ANY_TH=2.16 PRIMER_INTERNAL_86_SELF_END_TH=0.00 PRIMER_INTERNAL_86_HAIRPIN_TH=41.60 PRIMER_INTERNAL_87_PENALTY=6.661393 PRIMER_INTERNAL_87_SEQUENCE=ACACACACACACACAGCACACAGGTA PRIMER_INTERNAL_87=35,26 PRIMER_INTERNAL_87_TM=60.661 PRIMER_INTERNAL_87_GC_PERCENT=50.000 PRIMER_INTERNAL_87_SELF_ANY_TH=0.00 PRIMER_INTERNAL_87_SELF_END_TH=0.00 PRIMER_INTERNAL_87_HAIRPIN_TH=0.00 PRIMER_INTERNAL_88_PENALTY=6.664685 PRIMER_INTERNAL_88_SEQUENCE=CACAGCACACAGGTATTAGTGGGCCA PRIMER_INTERNAL_88=46,26 PRIMER_INTERNAL_88_TM=60.665 PRIMER_INTERNAL_88_GC_PERCENT=53.846 PRIMER_INTERNAL_88_SELF_ANY_TH=8.63 PRIMER_INTERNAL_88_SELF_END_TH=1.50 PRIMER_INTERNAL_88_HAIRPIN_TH=32.68 PRIMER_INTERNAL_89_PENALTY=6.664922 PRIMER_INTERNAL_89_SEQUENCE=ACACAGCACACAGGTATTAGTGGGCC PRIMER_INTERNAL_89=45,26 PRIMER_INTERNAL_89_TM=60.665 PRIMER_INTERNAL_89_GC_PERCENT=53.846 PRIMER_INTERNAL_89_SELF_ANY_TH=8.63 PRIMER_INTERNAL_89_SELF_END_TH=0.00 PRIMER_INTERNAL_89_HAIRPIN_TH=32.68 PRIMER_INTERNAL_90_PENALTY=6.679783 PRIMER_INTERNAL_90_SEQUENCE=CCCGACCCAAATCGATAGCTACGAT PRIMER_INTERNAL_90=78,25 PRIMER_INTERNAL_90_TM=58.320 PRIMER_INTERNAL_90_GC_PERCENT=52.000 PRIMER_INTERNAL_90_SELF_ANY_TH=14.48 PRIMER_INTERNAL_90_SELF_END_TH=14.48 PRIMER_INTERNAL_90_HAIRPIN_TH=46.54 PRIMER_INTERNAL_91_PENALTY=6.679783 PRIMER_INTERNAL_91_SEQUENCE=ATCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_91=76,25 PRIMER_INTERNAL_91_TM=58.320 PRIMER_INTERNAL_91_GC_PERCENT=52.000 PRIMER_INTERNAL_91_SELF_ANY_TH=5.17 PRIMER_INTERNAL_91_SELF_END_TH=5.17 PRIMER_INTERNAL_91_HAIRPIN_TH=41.28 PRIMER_INTERNAL_92_PENALTY=6.695664 PRIMER_INTERNAL_92_SEQUENCE=TATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_92=59,24 PRIMER_INTERNAL_92_TM=57.304 PRIMER_INTERNAL_92_GC_PERCENT=50.000 PRIMER_INTERNAL_92_SELF_ANY_TH=0.00 PRIMER_INTERNAL_92_SELF_END_TH=0.00 PRIMER_INTERNAL_92_HAIRPIN_TH=41.96 PRIMER_INTERNAL_93_PENALTY=6.741797 PRIMER_INTERNAL_93_SEQUENCE=GTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_93=58,24 PRIMER_INTERNAL_93_TM=57.258 PRIMER_INTERNAL_93_GC_PERCENT=54.167 PRIMER_INTERNAL_93_SELF_ANY_TH=4.28 PRIMER_INTERNAL_93_SELF_END_TH=0.00 PRIMER_INTERNAL_93_HAIRPIN_TH=37.07 PRIMER_INTERNAL_94_PENALTY=6.754240 PRIMER_INTERNAL_94_SEQUENCE=TATTAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_94=59,25 PRIMER_INTERNAL_94_TM=58.246 PRIMER_INTERNAL_94_GC_PERCENT=52.000 PRIMER_INTERNAL_94_SELF_ANY_TH=3.57 PRIMER_INTERNAL_94_SELF_END_TH=3.02 PRIMER_INTERNAL_94_HAIRPIN_TH=41.60 PRIMER_INTERNAL_95_PENALTY=6.754240 PRIMER_INTERNAL_95_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_95=58,25 PRIMER_INTERNAL_95_TM=58.246 PRIMER_INTERNAL_95_GC_PERCENT=52.000 PRIMER_INTERNAL_95_SELF_ANY_TH=0.00 PRIMER_INTERNAL_95_SELF_END_TH=0.00 PRIMER_INTERNAL_95_HAIRPIN_TH=41.96 PRIMER_INTERNAL_96_PENALTY=6.756422 PRIMER_INTERNAL_96_SEQUENCE=TCCCGACCCAAATCGATAGCTACGAT PRIMER_INTERNAL_96=77,26 PRIMER_INTERNAL_96_TM=59.244 PRIMER_INTERNAL_96_GC_PERCENT=50.000 PRIMER_INTERNAL_96_SELF_ANY_TH=14.48 PRIMER_INTERNAL_96_SELF_END_TH=14.48 PRIMER_INTERNAL_96_HAIRPIN_TH=46.54 PRIMER_INTERNAL_97_PENALTY=6.756422 PRIMER_INTERNAL_97_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_97=76,26 PRIMER_INTERNAL_97_TM=59.244 PRIMER_INTERNAL_97_GC_PERCENT=50.000 PRIMER_INTERNAL_97_SELF_ANY_TH=12.68 PRIMER_INTERNAL_97_SELF_END_TH=12.68 PRIMER_INTERNAL_97_HAIRPIN_TH=41.28 PRIMER_INTERNAL_98_PENALTY=6.791532 PRIMER_INTERNAL_98_SEQUENCE=AGCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_98=49,25 PRIMER_INTERNAL_98_TM=58.208 PRIMER_INTERNAL_98_GC_PERCENT=48.000 PRIMER_INTERNAL_98_SELF_ANY_TH=8.63 PRIMER_INTERNAL_98_SELF_END_TH=0.00 PRIMER_INTERNAL_98_HAIRPIN_TH=32.68 PRIMER_INTERNAL_99_PENALTY=6.793369 PRIMER_INTERNAL_99_SEQUENCE=GCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_99=50,24 PRIMER_INTERNAL_99_TM=57.207 PRIMER_INTERNAL_99_GC_PERCENT=50.000 PRIMER_INTERNAL_99_SELF_ANY_TH=2.67 PRIMER_INTERNAL_99_SELF_END_TH=0.00 PRIMER_INTERNAL_99_HAIRPIN_TH=28.81 PRIMER_INTERNAL_100_PENALTY=6.804314 PRIMER_INTERNAL_100_SEQUENCE=CATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_100=70,24 PRIMER_INTERNAL_100_TM=57.196 PRIMER_INTERNAL_100_GC_PERCENT=50.000 PRIMER_INTERNAL_100_SELF_ANY_TH=0.00 PRIMER_INTERNAL_100_SELF_END_TH=0.00 PRIMER_INTERNAL_100_HAIRPIN_TH=41.60 PRIMER_INTERNAL_101_PENALTY=6.828710 PRIMER_INTERNAL_101_SEQUENCE=GTGGGCCATTCGATCCCGACCCAA PRIMER_INTERNAL_101=64,24 PRIMER_INTERNAL_101_TM=62.829 PRIMER_INTERNAL_101_GC_PERCENT=62.500 PRIMER_INTERNAL_101_SELF_ANY_TH=8.58 PRIMER_INTERNAL_101_SELF_END_TH=0.00 PRIMER_INTERNAL_101_HAIRPIN_TH=43.79 PRIMER_INTERNAL_102_PENALTY=6.841708 PRIMER_INTERNAL_102_SEQUENCE=CCCGACCCAAATCGATAGCTACGATG PRIMER_INTERNAL_102=78,26 PRIMER_INTERNAL_102_TM=59.158 PRIMER_INTERNAL_102_GC_PERCENT=53.846 PRIMER_INTERNAL_102_SELF_ANY_TH=5.23 PRIMER_INTERNAL_102_SELF_END_TH=0.00 PRIMER_INTERNAL_102_HAIRPIN_TH=43.02 PRIMER_INTERNAL_103_PENALTY=6.895596 PRIMER_INTERNAL_103_SEQUENCE=GGCCATTCGATCCCGACCCAAATCG PRIMER_INTERNAL_103=67,25 PRIMER_INTERNAL_103_TM=61.896 PRIMER_INTERNAL_103_GC_PERCENT=60.000 PRIMER_INTERNAL_103_SELF_ANY_TH=0.00 PRIMER_INTERNAL_103_SELF_END_TH=0.00 PRIMER_INTERNAL_103_HAIRPIN_TH=41.60 PRIMER_INTERNAL_104_PENALTY=6.905174 PRIMER_INTERNAL_104_SEQUENCE=CGATCCCGACCCAAATCGATAGCTA PRIMER_INTERNAL_104=74,25 PRIMER_INTERNAL_104_TM=58.095 PRIMER_INTERNAL_104_GC_PERCENT=52.000 PRIMER_INTERNAL_104_SELF_ANY_TH=0.00 PRIMER_INTERNAL_104_SELF_END_TH=0.00 PRIMER_INTERNAL_104_HAIRPIN_TH=41.28 PRIMER_INTERNAL_105_PENALTY=6.905408 PRIMER_INTERNAL_105_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_105=58,26 PRIMER_INTERNAL_105_TM=59.095 PRIMER_INTERNAL_105_GC_PERCENT=53.846 PRIMER_INTERNAL_105_SELF_ANY_TH=3.57 PRIMER_INTERNAL_105_SELF_END_TH=3.02 PRIMER_INTERNAL_105_HAIRPIN_TH=41.60 PRIMER_INTERNAL_106_PENALTY=6.917157 PRIMER_INTERNAL_106_SEQUENCE=ACACACACACACAGCACACAGGTATT PRIMER_INTERNAL_106=37,26 PRIMER_INTERNAL_106_TM=59.083 PRIMER_INTERNAL_106_GC_PERCENT=46.154 PRIMER_INTERNAL_106_SELF_ANY_TH=0.00 PRIMER_INTERNAL_106_SELF_END_TH=0.00 PRIMER_INTERNAL_106_HAIRPIN_TH=0.00 PRIMER_INTERNAL_107_PENALTY=6.924766 PRIMER_INTERNAL_107_SEQUENCE=CAGCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_107=48,26 PRIMER_INTERNAL_107_TM=59.075 PRIMER_INTERNAL_107_GC_PERCENT=50.000 PRIMER_INTERNAL_107_SELF_ANY_TH=8.63 PRIMER_INTERNAL_107_SELF_END_TH=0.00 PRIMER_INTERNAL_107_HAIRPIN_TH=32.68 PRIMER_INTERNAL_108_PENALTY=6.956454 PRIMER_INTERNAL_108_SEQUENCE=AGACATGACACTGACGATGCAGAC PRIMER_INTERNAL_108=10,24 PRIMER_INTERNAL_108_TM=57.044 PRIMER_INTERNAL_108_GC_PERCENT=50.000 PRIMER_INTERNAL_108_SELF_ANY_TH=1.44 PRIMER_INTERNAL_108_SELF_END_TH=0.00 PRIMER_INTERNAL_108_HAIRPIN_TH=0.00 PRIMER_INTERNAL_109_PENALTY=6.975446 PRIMER_INTERNAL_109_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTA PRIMER_INTERNAL_109=73,26 PRIMER_INTERNAL_109_TM=59.025 PRIMER_INTERNAL_109_GC_PERCENT=50.000 PRIMER_INTERNAL_109_SELF_ANY_TH=0.30 PRIMER_INTERNAL_109_SELF_END_TH=0.00 PRIMER_INTERNAL_109_HAIRPIN_TH=41.28 PRIMER_INTERNAL_110_PENALTY=7.015932 PRIMER_INTERNAL_110_SEQUENCE=CCCGACCCAAATCGATAGCTACGATGA PRIMER_INTERNAL_110=78,27 PRIMER_INTERNAL_110_TM=60.016 PRIMER_INTERNAL_110_GC_PERCENT=51.852 PRIMER_INTERNAL_110_SELF_ANY_TH=7.53 PRIMER_INTERNAL_110_SELF_END_TH=0.00 PRIMER_INTERNAL_110_HAIRPIN_TH=43.02 PRIMER_INTERNAL_111_PENALTY=7.015932 PRIMER_INTERNAL_111_SEQUENCE=TCCCGACCCAAATCGATAGCTACGATG PRIMER_INTERNAL_111=77,27 PRIMER_INTERNAL_111_TM=60.016 PRIMER_INTERNAL_111_GC_PERCENT=51.852 PRIMER_INTERNAL_111_SELF_ANY_TH=5.23 PRIMER_INTERNAL_111_SELF_END_TH=0.00 PRIMER_INTERNAL_111_HAIRPIN_TH=43.02 PRIMER_INTERNAL_112_PENALTY=7.051167 PRIMER_INTERNAL_112_SEQUENCE=TTAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_112=61,25 PRIMER_INTERNAL_112_TM=62.051 PRIMER_INTERNAL_112_GC_PERCENT=60.000 PRIMER_INTERNAL_112_SELF_ANY_TH=5.61 PRIMER_INTERNAL_112_SELF_END_TH=2.93 PRIMER_INTERNAL_112_HAIRPIN_TH=41.60 PRIMER_INTERNAL_113_PENALTY=7.056335 PRIMER_INTERNAL_113_SEQUENCE=GATCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_113=75,26 PRIMER_INTERNAL_113_TM=58.944 PRIMER_INTERNAL_113_GC_PERCENT=53.846 PRIMER_INTERNAL_113_SELF_ANY_TH=5.17 PRIMER_INTERNAL_113_SELF_END_TH=5.17 PRIMER_INTERNAL_113_HAIRPIN_TH=41.28 PRIMER_INTERNAL_114_PENALTY=7.056335 PRIMER_INTERNAL_114_SEQUENCE=CGATCCCGACCCAAATCGATAGCTAC PRIMER_INTERNAL_114=74,26 PRIMER_INTERNAL_114_TM=58.944 PRIMER_INTERNAL_114_GC_PERCENT=53.846 PRIMER_INTERNAL_114_SELF_ANY_TH=0.00 PRIMER_INTERNAL_114_SELF_END_TH=0.00 PRIMER_INTERNAL_114_HAIRPIN_TH=41.28 PRIMER_INTERNAL_115_PENALTY=7.066919 PRIMER_INTERNAL_115_SEQUENCE=CACACACACACAGCACACAGGTATT PRIMER_INTERNAL_115=38,25 PRIMER_INTERNAL_115_TM=57.933 PRIMER_INTERNAL_115_GC_PERCENT=48.000 PRIMER_INTERNAL_115_SELF_ANY_TH=0.00 PRIMER_INTERNAL_115_SELF_END_TH=0.00 PRIMER_INTERNAL_115_HAIRPIN_TH=0.00 PRIMER_INTERNAL_116_PENALTY=7.070364 PRIMER_INTERNAL_116_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCC PRIMER_INTERNAL_116=56,25 PRIMER_INTERNAL_116_TM=57.930 PRIMER_INTERNAL_116_GC_PERCENT=52.000 PRIMER_INTERNAL_116_SELF_ANY_TH=4.31 PRIMER_INTERNAL_116_SELF_END_TH=0.37 PRIMER_INTERNAL_116_HAIRPIN_TH=0.00 PRIMER_INTERNAL_117_PENALTY=7.079469 PRIMER_INTERNAL_117_SEQUENCE=GCACACAGGTATTAGTGGGCCATTC PRIMER_INTERNAL_117=50,25 PRIMER_INTERNAL_117_TM=57.921 PRIMER_INTERNAL_117_GC_PERCENT=52.000 PRIMER_INTERNAL_117_SELF_ANY_TH=2.67 PRIMER_INTERNAL_117_SELF_END_TH=0.00 PRIMER_INTERNAL_117_HAIRPIN_TH=28.81 PRIMER_INTERNAL_118_PENALTY=7.094172 PRIMER_INTERNAL_118_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCCC PRIMER_INTERNAL_118=54,27 PRIMER_INTERNAL_118_TM=59.906 PRIMER_INTERNAL_118_GC_PERCENT=51.852 PRIMER_INTERNAL_118_SELF_ANY_TH=4.31 PRIMER_INTERNAL_118_SELF_END_TH=0.37 PRIMER_INTERNAL_118_HAIRPIN_TH=0.00 PRIMER_INTERNAL_119_PENALTY=7.124042 PRIMER_INTERNAL_119_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_119=71,26 PRIMER_INTERNAL_119_TM=58.876 PRIMER_INTERNAL_119_GC_PERCENT=50.000 PRIMER_INTERNAL_119_SELF_ANY_TH=0.00 PRIMER_INTERNAL_119_SELF_END_TH=0.00 PRIMER_INTERNAL_119_HAIRPIN_TH=41.60 PRIMER_INTERNAL_120_PENALTY=7.139284 PRIMER_INTERNAL_120_SEQUENCE=CACACACACACACAGCACACAGGTATT PRIMER_INTERNAL_120=36,27 PRIMER_INTERNAL_120_TM=59.861 PRIMER_INTERNAL_120_GC_PERCENT=48.148 PRIMER_INTERNAL_120_SELF_ANY_TH=0.00 PRIMER_INTERNAL_120_SELF_END_TH=0.00 PRIMER_INTERNAL_120_HAIRPIN_TH=0.00 PRIMER_INTERNAL_121_PENALTY=7.145199 PRIMER_INTERNAL_121_SEQUENCE=GCCATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_121=68,26 PRIMER_INTERNAL_121_TM=61.145 PRIMER_INTERNAL_121_GC_PERCENT=53.846 PRIMER_INTERNAL_121_SELF_ANY_TH=0.00 PRIMER_INTERNAL_121_SELF_END_TH=0.00 PRIMER_INTERNAL_121_HAIRPIN_TH=41.60 PRIMER_INTERNAL_122_PENALTY=7.145368 PRIMER_INTERNAL_122_SEQUENCE=AGCACACAGGTATTAGTGGGCCATTC PRIMER_INTERNAL_122=49,26 PRIMER_INTERNAL_122_TM=58.855 PRIMER_INTERNAL_122_GC_PERCENT=50.000 PRIMER_INTERNAL_122_SELF_ANY_TH=8.63 PRIMER_INTERNAL_122_SELF_END_TH=0.00 PRIMER_INTERNAL_122_HAIRPIN_TH=32.68 PRIMER_INTERNAL_123_PENALTY=7.171756 PRIMER_INTERNAL_123_SEQUENCE=ACAGCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_123=47,27 PRIMER_INTERNAL_123_TM=60.172 PRIMER_INTERNAL_123_GC_PERCENT=48.148 PRIMER_INTERNAL_123_SELF_ANY_TH=8.63 PRIMER_INTERNAL_123_SELF_END_TH=0.00 PRIMER_INTERNAL_123_HAIRPIN_TH=32.68 PRIMER_INTERNAL_124_PENALTY=7.192525 PRIMER_INTERNAL_124_SEQUENCE=GATCCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_124=75,27 PRIMER_INTERNAL_124_TM=59.807 PRIMER_INTERNAL_124_GC_PERCENT=51.852 PRIMER_INTERNAL_124_SELF_ANY_TH=12.68 PRIMER_INTERNAL_124_SELF_END_TH=12.68 PRIMER_INTERNAL_124_HAIRPIN_TH=41.28 PRIMER_INTERNAL_125_PENALTY=7.192525 PRIMER_INTERNAL_125_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTAC PRIMER_INTERNAL_125=73,27 PRIMER_INTERNAL_125_TM=59.807 PRIMER_INTERNAL_125_GC_PERCENT=51.852 PRIMER_INTERNAL_125_SELF_ANY_TH=0.30 PRIMER_INTERNAL_125_SELF_END_TH=0.00 PRIMER_INTERNAL_125_HAIRPIN_TH=41.28 PRIMER_INTERNAL_126_PENALTY=7.193259 PRIMER_INTERNAL_126_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCC PRIMER_INTERNAL_126=55,26 PRIMER_INTERNAL_126_TM=58.807 PRIMER_INTERNAL_126_GC_PERCENT=53.846 PRIMER_INTERNAL_126_SELF_ANY_TH=4.31 PRIMER_INTERNAL_126_SELF_END_TH=0.37 PRIMER_INTERNAL_126_HAIRPIN_TH=0.00 PRIMER_INTERNAL_127_PENALTY=7.239217 PRIMER_INTERNAL_127_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGA PRIMER_INTERNAL_127=52,25 PRIMER_INTERNAL_127_TM=57.761 PRIMER_INTERNAL_127_GC_PERCENT=48.000 PRIMER_INTERNAL_127_SELF_ANY_TH=0.00 PRIMER_INTERNAL_127_SELF_END_TH=0.00 PRIMER_INTERNAL_127_HAIRPIN_TH=0.00 PRIMER_INTERNAL_128_PENALTY=7.243997 PRIMER_INTERNAL_128_SEQUENCE=TGGGCCATTCGATCCCGACCCAAAT PRIMER_INTERNAL_128=65,25 PRIMER_INTERNAL_128_TM=62.244 PRIMER_INTERNAL_128_GC_PERCENT=56.000 PRIMER_INTERNAL_128_SELF_ANY_TH=8.58 PRIMER_INTERNAL_128_SELF_END_TH=0.00 PRIMER_INTERNAL_128_HAIRPIN_TH=41.29 PRIMER_INTERNAL_129_PENALTY=7.246964 PRIMER_INTERNAL_129_SEQUENCE=ACACACAGCACACAGGTATTAGTGGG PRIMER_INTERNAL_129=43,26 PRIMER_INTERNAL_129_TM=58.753 PRIMER_INTERNAL_129_GC_PERCENT=50.000 PRIMER_INTERNAL_129_SELF_ANY_TH=0.00 PRIMER_INTERNAL_129_SELF_END_TH=0.00 PRIMER_INTERNAL_129_HAIRPIN_TH=0.00 PRIMER_INTERNAL_130_PENALTY=7.250024 PRIMER_INTERNAL_130_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_130=71,27 PRIMER_INTERNAL_130_TM=59.750 PRIMER_INTERNAL_130_GC_PERCENT=48.148 PRIMER_INTERNAL_130_SELF_ANY_TH=0.00 PRIMER_INTERNAL_130_SELF_END_TH=0.00 PRIMER_INTERNAL_130_HAIRPIN_TH=41.60 PRIMER_INTERNAL_131_PENALTY=7.293083 PRIMER_INTERNAL_131_SEQUENCE=CACACAGGTATTAGTGGGCCATTCG PRIMER_INTERNAL_131=51,25 PRIMER_INTERNAL_131_TM=57.707 PRIMER_INTERNAL_131_GC_PERCENT=52.000 PRIMER_INTERNAL_131_SELF_ANY_TH=0.00 PRIMER_INTERNAL_131_SELF_END_TH=0.00 PRIMER_INTERNAL_131_HAIRPIN_TH=0.00 PRIMER_INTERNAL_132_PENALTY=7.342607 PRIMER_INTERNAL_132_SEQUENCE=CATTCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_132=70,27 PRIMER_INTERNAL_132_TM=59.657 PRIMER_INTERNAL_132_GC_PERCENT=51.852 PRIMER_INTERNAL_132_SELF_ANY_TH=0.00 PRIMER_INTERNAL_132_SELF_END_TH=0.00 PRIMER_INTERNAL_132_HAIRPIN_TH=41.60 PRIMER_INTERNAL_133_PENALTY=7.350773 PRIMER_INTERNAL_133_SEQUENCE=CAGCACACAGGTATTAGTGGGCCATTC PRIMER_INTERNAL_133=48,27 PRIMER_INTERNAL_133_TM=59.649 PRIMER_INTERNAL_133_GC_PERCENT=51.852 PRIMER_INTERNAL_133_SELF_ANY_TH=8.63 PRIMER_INTERNAL_133_SELF_END_TH=0.00 PRIMER_INTERNAL_133_HAIRPIN_TH=32.68 PRIMER_INTERNAL_134_PENALTY=7.354919 PRIMER_INTERNAL_134_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGA PRIMER_INTERNAL_134=51,26 PRIMER_INTERNAL_134_TM=58.645 PRIMER_INTERNAL_134_GC_PERCENT=50.000 PRIMER_INTERNAL_134_SELF_ANY_TH=0.00 PRIMER_INTERNAL_134_SELF_END_TH=0.00 PRIMER_INTERNAL_134_HAIRPIN_TH=0.00 PRIMER_INTERNAL_135_PENALTY=7.401440 PRIMER_INTERNAL_135_SEQUENCE=CCATTCGATCCCGACCCAAATCGATA PRIMER_INTERNAL_135=69,26 PRIMER_INTERNAL_135_TM=58.599 PRIMER_INTERNAL_135_GC_PERCENT=50.000 PRIMER_INTERNAL_135_SELF_ANY_TH=0.00 PRIMER_INTERNAL_135_SELF_END_TH=0.00 PRIMER_INTERNAL_135_HAIRPIN_TH=41.60 PRIMER_INTERNAL_136_PENALTY=7.408271 PRIMER_INTERNAL_136_SEQUENCE=CACACAGCACACAGGTATTAGTGGG PRIMER_INTERNAL_136=44,25 PRIMER_INTERNAL_136_TM=57.592 PRIMER_INTERNAL_136_GC_PERCENT=52.000 PRIMER_INTERNAL_136_SELF_ANY_TH=0.00 PRIMER_INTERNAL_136_SELF_END_TH=0.00 PRIMER_INTERNAL_136_HAIRPIN_TH=0.00 PRIMER_INTERNAL_137_PENALTY=7.451841 PRIMER_INTERNAL_137_SEQUENCE=CACACACAGCACACAGGTATTAGTGGG PRIMER_INTERNAL_137=42,27 PRIMER_INTERNAL_137_TM=59.548 PRIMER_INTERNAL_137_GC_PERCENT=51.852 PRIMER_INTERNAL_137_SELF_ANY_TH=0.00 PRIMER_INTERNAL_137_SELF_END_TH=0.00 PRIMER_INTERNAL_137_HAIRPIN_TH=0.00 PRIMER_INTERNAL_138_PENALTY=7.534114 PRIMER_INTERNAL_138_SEQUENCE=CCGACCCAAATCGATAGCTACGATG PRIMER_INTERNAL_138=79,25 PRIMER_INTERNAL_138_TM=57.466 PRIMER_INTERNAL_138_GC_PERCENT=52.000 PRIMER_INTERNAL_138_SELF_ANY_TH=5.23 PRIMER_INTERNAL_138_SELF_END_TH=0.00 PRIMER_INTERNAL_138_HAIRPIN_TH=43.02 PRIMER_INTERNAL_139_PENALTY=7.537719 PRIMER_INTERNAL_139_SEQUENCE=CGACCCAAATCGATAGCTACGATGACG PRIMER_INTERNAL_139=80,27 PRIMER_INTERNAL_139_TM=59.462 PRIMER_INTERNAL_139_GC_PERCENT=51.852 PRIMER_INTERNAL_139_SELF_ANY_TH=6.52 PRIMER_INTERNAL_139_SELF_END_TH=4.23 PRIMER_INTERNAL_139_HAIRPIN_TH=43.02 PRIMER_INTERNAL_140_PENALTY=7.602591 PRIMER_INTERNAL_140_SEQUENCE=CCGACCCAAATCGATAGCTACGATGA PRIMER_INTERNAL_140=79,26 PRIMER_INTERNAL_140_TM=58.397 PRIMER_INTERNAL_140_GC_PERCENT=50.000 PRIMER_INTERNAL_140_SELF_ANY_TH=7.53 PRIMER_INTERNAL_140_SELF_END_TH=0.00 PRIMER_INTERNAL_140_HAIRPIN_TH=43.02 PRIMER_INTERNAL_141_PENALTY=7.672898 PRIMER_INTERNAL_141_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGAT PRIMER_INTERNAL_141=76,27 PRIMER_INTERNAL_141_TM=59.327 PRIMER_INTERNAL_141_GC_PERCENT=48.148 PRIMER_INTERNAL_141_SELF_ANY_TH=14.48 PRIMER_INTERNAL_141_SELF_END_TH=14.48 PRIMER_INTERNAL_141_HAIRPIN_TH=46.54 PRIMER_INTERNAL_142_PENALTY=7.693260 PRIMER_INTERNAL_142_SEQUENCE=GCCATTCGATCCCGACCCAAATCGATA PRIMER_INTERNAL_142=68,27 PRIMER_INTERNAL_142_TM=60.693 PRIMER_INTERNAL_142_GC_PERCENT=51.852 PRIMER_INTERNAL_142_SELF_ANY_TH=0.00 PRIMER_INTERNAL_142_SELF_END_TH=0.00 PRIMER_INTERNAL_142_HAIRPIN_TH=41.60 PRIMER_INTERNAL_143_PENALTY=7.693499 PRIMER_INTERNAL_143_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGACC PRIMER_INTERNAL_143=58,27 PRIMER_INTERNAL_143_TM=60.693 PRIMER_INTERNAL_143_GC_PERCENT=55.556 PRIMER_INTERNAL_143_SELF_ANY_TH=5.61 PRIMER_INTERNAL_143_SELF_END_TH=0.00 PRIMER_INTERNAL_143_HAIRPIN_TH=41.60 PRIMER_INTERNAL_144_PENALTY=7.693499 PRIMER_INTERNAL_144_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_144=57,27 PRIMER_INTERNAL_144_TM=60.693 PRIMER_INTERNAL_144_GC_PERCENT=55.556 PRIMER_INTERNAL_144_SELF_ANY_TH=3.57 PRIMER_INTERNAL_144_SELF_END_TH=3.02 PRIMER_INTERNAL_144_HAIRPIN_TH=41.60 PRIMER_INTERNAL_145_PENALTY=7.694218 PRIMER_INTERNAL_145_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_145=55,27 PRIMER_INTERNAL_145_TM=60.694 PRIMER_INTERNAL_145_GC_PERCENT=55.556 PRIMER_INTERNAL_145_SELF_ANY_TH=4.31 PRIMER_INTERNAL_145_SELF_END_TH=0.00 PRIMER_INTERNAL_145_HAIRPIN_TH=37.07 PRIMER_INTERNAL_146_PENALTY=7.696389 PRIMER_INTERNAL_146_SEQUENCE=ACACACACACACACAGCACACAGGTAT PRIMER_INTERNAL_146=35,27 PRIMER_INTERNAL_146_TM=60.696 PRIMER_INTERNAL_146_GC_PERCENT=48.148 PRIMER_INTERNAL_146_SELF_ANY_TH=0.00 PRIMER_INTERNAL_146_SELF_END_TH=0.00 PRIMER_INTERNAL_146_HAIRPIN_TH=0.00 PRIMER_INTERNAL_147_PENALTY=7.699801 PRIMER_INTERNAL_147_SEQUENCE=CACAGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_147=46,27 PRIMER_INTERNAL_147_TM=60.700 PRIMER_INTERNAL_147_GC_PERCENT=51.852 PRIMER_INTERNAL_147_SELF_ANY_TH=8.63 PRIMER_INTERNAL_147_SELF_END_TH=0.00 PRIMER_INTERNAL_147_HAIRPIN_TH=32.68 PRIMER_INTERNAL_148_PENALTY=7.723400 PRIMER_INTERNAL_148_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCTA PRIMER_INTERNAL_148=72,27 PRIMER_INTERNAL_148_TM=59.277 PRIMER_INTERNAL_148_GC_PERCENT=48.148 PRIMER_INTERNAL_148_SELF_ANY_TH=0.00 PRIMER_INTERNAL_148_SELF_END_TH=0.00 PRIMER_INTERNAL_148_HAIRPIN_TH=41.60 PRIMER_INTERNAL_149_PENALTY=7.748120 PRIMER_INTERNAL_149_SEQUENCE=GCACACAGGTATTAGTGGGCCATTCGA PRIMER_INTERNAL_149=50,27 PRIMER_INTERNAL_149_TM=60.748 PRIMER_INTERNAL_149_GC_PERCENT=51.852 PRIMER_INTERNAL_149_SELF_ANY_TH=2.67 PRIMER_INTERNAL_149_SELF_END_TH=0.00 PRIMER_INTERNAL_149_HAIRPIN_TH=28.81 PRIMER_INTERNAL_150_PENALTY=7.749187 PRIMER_INTERNAL_150_SEQUENCE=AGCACACAGGTATTAGTGGGCCATTCG PRIMER_INTERNAL_150=49,27 PRIMER_INTERNAL_150_TM=60.749 PRIMER_INTERNAL_150_GC_PERCENT=51.852 PRIMER_INTERNAL_150_SELF_ANY_TH=8.63 PRIMER_INTERNAL_150_SELF_END_TH=0.00 PRIMER_INTERNAL_150_HAIRPIN_TH=32.68 PRIMER_INTERNAL_151_PENALTY=7.753661 PRIMER_INTERNAL_151_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCC PRIMER_INTERNAL_151=54,26 PRIMER_INTERNAL_151_TM=58.246 PRIMER_INTERNAL_151_GC_PERCENT=50.000 PRIMER_INTERNAL_151_SELF_ANY_TH=2.01 PRIMER_INTERNAL_151_SELF_END_TH=1.68 PRIMER_INTERNAL_151_HAIRPIN_TH=0.00 PRIMER_INTERNAL_152_PENALTY=7.785822 PRIMER_INTERNAL_152_SEQUENCE=CGATCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_152=74,27 PRIMER_INTERNAL_152_TM=60.786 PRIMER_INTERNAL_152_GC_PERCENT=55.556 PRIMER_INTERNAL_152_SELF_ANY_TH=5.17 PRIMER_INTERNAL_152_SELF_END_TH=5.17 PRIMER_INTERNAL_152_HAIRPIN_TH=41.28 PRIMER_INTERNAL_153_PENALTY=7.802259 PRIMER_INTERNAL_153_SEQUENCE=CCGACCCAAATCGATAGCTACGATGAC PRIMER_INTERNAL_153=79,27 PRIMER_INTERNAL_153_TM=59.198 PRIMER_INTERNAL_153_GC_PERCENT=51.852 PRIMER_INTERNAL_153_SELF_ANY_TH=5.43 PRIMER_INTERNAL_153_SELF_END_TH=0.00 PRIMER_INTERNAL_153_HAIRPIN_TH=43.02 PRIMER_INTERNAL_154_PENALTY=7.812760 PRIMER_INTERNAL_154_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_154=56,27 PRIMER_INTERNAL_154_TM=60.813 PRIMER_INTERNAL_154_GC_PERCENT=51.852 PRIMER_INTERNAL_154_SELF_ANY_TH=4.31 PRIMER_INTERNAL_154_SELF_END_TH=0.00 PRIMER_INTERNAL_154_HAIRPIN_TH=41.96 PRIMER_INTERNAL_155_PENALTY=7.812835 PRIMER_INTERNAL_155_SEQUENCE=CCATTCGATCCCGACCCAAATCGATAG PRIMER_INTERNAL_155=69,27 PRIMER_INTERNAL_155_TM=59.187 PRIMER_INTERNAL_155_GC_PERCENT=51.852 PRIMER_INTERNAL_155_SELF_ANY_TH=0.00 PRIMER_INTERNAL_155_SELF_END_TH=0.00 PRIMER_INTERNAL_155_HAIRPIN_TH=41.60 PRIMER_INTERNAL_156_PENALTY=7.853399 PRIMER_INTERNAL_156_SEQUENCE=ACACACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_156=43,27 PRIMER_INTERNAL_156_TM=60.853 PRIMER_INTERNAL_156_GC_PERCENT=51.852 PRIMER_INTERNAL_156_SELF_ANY_TH=5.21 PRIMER_INTERNAL_156_SELF_END_TH=5.21 PRIMER_INTERNAL_156_HAIRPIN_TH=33.51 PRIMER_INTERNAL_157_PENALTY=7.928092 PRIMER_INTERNAL_157_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATCC PRIMER_INTERNAL_157=53,27 PRIMER_INTERNAL_157_TM=59.072 PRIMER_INTERNAL_157_GC_PERCENT=51.852 PRIMER_INTERNAL_157_SELF_ANY_TH=2.01 PRIMER_INTERNAL_157_SELF_END_TH=1.68 PRIMER_INTERNAL_157_HAIRPIN_TH=0.00 PRIMER_INTERNAL_158_PENALTY=7.938713 PRIMER_INTERNAL_158_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCC PRIMER_INTERNAL_158=55,25 PRIMER_INTERNAL_158_TM=57.061 PRIMER_INTERNAL_158_GC_PERCENT=52.000 PRIMER_INTERNAL_158_SELF_ANY_TH=2.01 PRIMER_INTERNAL_158_SELF_END_TH=1.68 PRIMER_INTERNAL_158_HAIRPIN_TH=0.00 PRIMER_INTERNAL_159_PENALTY=7.949148 PRIMER_INTERNAL_159_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAA PRIMER_INTERNAL_159=64,25 PRIMER_INTERNAL_159_TM=62.949 PRIMER_INTERNAL_159_GC_PERCENT=60.000 PRIMER_INTERNAL_159_SELF_ANY_TH=8.58 PRIMER_INTERNAL_159_SELF_END_TH=0.00 PRIMER_INTERNAL_159_HAIRPIN_TH=43.79 PRIMER_INTERNAL_160_PENALTY=7.979746 PRIMER_INTERNAL_160_SEQUENCE=ACACACACAGCACACAGGTATTAGTGG PRIMER_INTERNAL_160=41,27 PRIMER_INTERNAL_160_TM=59.020 PRIMER_INTERNAL_160_GC_PERCENT=48.148 PRIMER_INTERNAL_160_SELF_ANY_TH=0.00 PRIMER_INTERNAL_160_SELF_END_TH=0.00 PRIMER_INTERNAL_160_HAIRPIN_TH=0.00 PRIMER_INTERNAL_161_PENALTY=7.992102 PRIMER_INTERNAL_161_SEQUENCE=TAGTGGGCCATTCGATCCCGACCCA PRIMER_INTERNAL_161=62,25 PRIMER_INTERNAL_161_TM=62.992 PRIMER_INTERNAL_161_GC_PERCENT=60.000 PRIMER_INTERNAL_161_SELF_ANY_TH=8.58 PRIMER_INTERNAL_161_SELF_END_TH=6.40 PRIMER_INTERNAL_161_HAIRPIN_TH=42.67 PRIMER_INTERNAL_162_PENALTY=7.993148 PRIMER_INTERNAL_162_SEQUENCE=ACACACAGCACACAGGTATTAGTGG PRIMER_INTERNAL_162=43,25 PRIMER_INTERNAL_162_TM=57.007 PRIMER_INTERNAL_162_GC_PERCENT=48.000 PRIMER_INTERNAL_162_SELF_ANY_TH=0.00 PRIMER_INTERNAL_162_SELF_END_TH=0.00 PRIMER_INTERNAL_162_HAIRPIN_TH=0.00 PRIMER_INTERNAL_163_PENALTY=8.038590 PRIMER_INTERNAL_163_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_163=60,26 PRIMER_INTERNAL_163_TM=62.039 PRIMER_INTERNAL_163_GC_PERCENT=57.692 PRIMER_INTERNAL_163_SELF_ANY_TH=5.61 PRIMER_INTERNAL_163_SELF_END_TH=2.93 PRIMER_INTERNAL_163_HAIRPIN_TH=41.60 PRIMER_INTERNAL_164_PENALTY=8.084641 PRIMER_INTERNAL_164_SEQUENCE=CACACACAGCACACAGGTATTAGTGG PRIMER_INTERNAL_164=42,26 PRIMER_INTERNAL_164_TM=57.915 PRIMER_INTERNAL_164_GC_PERCENT=50.000 PRIMER_INTERNAL_164_SELF_ANY_TH=0.00 PRIMER_INTERNAL_164_SELF_END_TH=0.00 PRIMER_INTERNAL_164_HAIRPIN_TH=0.00 PRIMER_INTERNAL_165_PENALTY=8.098597 PRIMER_INTERNAL_165_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGAT PRIMER_INTERNAL_165=52,26 PRIMER_INTERNAL_165_TM=57.901 PRIMER_INTERNAL_165_GC_PERCENT=46.154 PRIMER_INTERNAL_165_SELF_ANY_TH=0.00 PRIMER_INTERNAL_165_SELF_END_TH=0.00 PRIMER_INTERNAL_165_HAIRPIN_TH=0.00 PRIMER_INTERNAL_166_PENALTY=8.106799 PRIMER_INTERNAL_166_SEQUENCE=TCAGTAGACATGACACTGACGATGCA PRIMER_INTERNAL_166=5,26 PRIMER_INTERNAL_166_TM=57.893 PRIMER_INTERNAL_166_GC_PERCENT=46.154 PRIMER_INTERNAL_166_SELF_ANY_TH=16.57 PRIMER_INTERNAL_166_SELF_END_TH=0.76 PRIMER_INTERNAL_166_HAIRPIN_TH=0.00 PRIMER_INTERNAL_167_PENALTY=8.251191 PRIMER_INTERNAL_167_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGAT PRIMER_INTERNAL_167=51,27 PRIMER_INTERNAL_167_TM=58.749 PRIMER_INTERNAL_167_GC_PERCENT=48.148 PRIMER_INTERNAL_167_SELF_ANY_TH=0.00 PRIMER_INTERNAL_167_SELF_END_TH=0.00 PRIMER_INTERNAL_167_HAIRPIN_TH=0.00 PRIMER_INTERNAL_168_PENALTY=8.275978 PRIMER_INTERNAL_168_SEQUENCE=GTCAGTAGACATGACACTGACGATGCA PRIMER_INTERNAL_168=4,27 PRIMER_INTERNAL_168_TM=58.724 PRIMER_INTERNAL_168_GC_PERCENT=48.148 PRIMER_INTERNAL_168_SELF_ANY_TH=22.48 PRIMER_INTERNAL_168_SELF_END_TH=5.75 PRIMER_INTERNAL_168_HAIRPIN_TH=41.09 PRIMER_INTERNAL_169_PENALTY=8.300145 PRIMER_INTERNAL_169_SEQUENCE=ACACACACACACAGCACACAGGTATTA PRIMER_INTERNAL_169=37,27 PRIMER_INTERNAL_169_TM=58.700 PRIMER_INTERNAL_169_GC_PERCENT=44.444 PRIMER_INTERNAL_169_SELF_ANY_TH=0.00 PRIMER_INTERNAL_169_SELF_END_TH=0.00 PRIMER_INTERNAL_169_HAIRPIN_TH=0.00 PRIMER_INTERNAL_170_PENALTY=8.330872 PRIMER_INTERNAL_170_SEQUENCE=AGTAGACATGACACTGACGATGCAGA PRIMER_INTERNAL_170=7,26 PRIMER_INTERNAL_170_TM=57.669 PRIMER_INTERNAL_170_GC_PERCENT=46.154 PRIMER_INTERNAL_170_SELF_ANY_TH=7.76 PRIMER_INTERNAL_170_SELF_END_TH=0.00 PRIMER_INTERNAL_170_HAIRPIN_TH=0.00 PRIMER_INTERNAL_171_PENALTY=8.369366 PRIMER_INTERNAL_171_SEQUENCE=CACACAGCACACAGGTATTAGTGGGCC PRIMER_INTERNAL_171=44,27 PRIMER_INTERNAL_171_TM=61.369 PRIMER_INTERNAL_171_GC_PERCENT=55.556 PRIMER_INTERNAL_171_SELF_ANY_TH=8.63 PRIMER_INTERNAL_171_SELF_END_TH=0.00 PRIMER_INTERNAL_171_HAIRPIN_TH=32.68 PRIMER_INTERNAL_172_PENALTY=8.376203 PRIMER_INTERNAL_172_SEQUENCE=GTAGACATGACACTGACGATGCAGAC PRIMER_INTERNAL_172=8,26 PRIMER_INTERNAL_172_TM=57.624 PRIMER_INTERNAL_172_GC_PERCENT=50.000 PRIMER_INTERNAL_172_SELF_ANY_TH=1.44 PRIMER_INTERNAL_172_SELF_END_TH=0.00 PRIMER_INTERNAL_172_HAIRPIN_TH=0.00 PRIMER_INTERNAL_173_PENALTY=8.376203 PRIMER_INTERNAL_173_SEQUENCE=GTCAGTAGACATGACACTGACGATGC PRIMER_INTERNAL_173=4,26 PRIMER_INTERNAL_173_TM=57.624 PRIMER_INTERNAL_173_GC_PERCENT=50.000 PRIMER_INTERNAL_173_SELF_ANY_TH=22.48 PRIMER_INTERNAL_173_SELF_END_TH=10.90 PRIMER_INTERNAL_173_HAIRPIN_TH=41.09 PRIMER_INTERNAL_174_PENALTY=8.379879 PRIMER_INTERNAL_174_SEQUENCE=CAGTAGACATGACACTGACGATGCAG PRIMER_INTERNAL_174=6,26 PRIMER_INTERNAL_174_TM=57.620 PRIMER_INTERNAL_174_GC_PERCENT=50.000 PRIMER_INTERNAL_174_SELF_ANY_TH=12.05 PRIMER_INTERNAL_174_SELF_END_TH=0.00 PRIMER_INTERNAL_174_HAIRPIN_TH=0.00 PRIMER_INTERNAL_175_PENALTY=8.418488 PRIMER_INTERNAL_175_SEQUENCE=CACACACACACAGCACACAGGTATTA PRIMER_INTERNAL_175=38,26 PRIMER_INTERNAL_175_TM=57.582 PRIMER_INTERNAL_175_GC_PERCENT=46.154 PRIMER_INTERNAL_175_SELF_ANY_TH=0.00 PRIMER_INTERNAL_175_SELF_END_TH=0.00 PRIMER_INTERNAL_175_HAIRPIN_TH=0.00 PRIMER_INTERNAL_176_PENALTY=8.423914 PRIMER_INTERNAL_176_SEQUENCE=GACCCAAATCGATAGCTACGATGACG PRIMER_INTERNAL_176=81,26 PRIMER_INTERNAL_176_TM=57.576 PRIMER_INTERNAL_176_GC_PERCENT=50.000 PRIMER_INTERNAL_176_SELF_ANY_TH=1.59 PRIMER_INTERNAL_176_SELF_END_TH=0.00 PRIMER_INTERNAL_176_HAIRPIN_TH=43.02 PRIMER_INTERNAL_177_PENALTY=8.423914 PRIMER_INTERNAL_177_SEQUENCE=CGACCCAAATCGATAGCTACGATGAC PRIMER_INTERNAL_177=80,26 PRIMER_INTERNAL_177_TM=57.576 PRIMER_INTERNAL_177_GC_PERCENT=50.000 PRIMER_INTERNAL_177_SELF_ANY_TH=2.38 PRIMER_INTERNAL_177_SELF_END_TH=0.00 PRIMER_INTERNAL_177_HAIRPIN_TH=43.02 PRIMER_INTERNAL_178_PENALTY=8.452836 PRIMER_INTERNAL_178_SEQUENCE=CATTCGATCCCGACCCAAATCGATAG PRIMER_INTERNAL_178=70,26 PRIMER_INTERNAL_178_TM=57.547 PRIMER_INTERNAL_178_GC_PERCENT=50.000 PRIMER_INTERNAL_178_SELF_ANY_TH=0.00 PRIMER_INTERNAL_178_SELF_END_TH=0.00 PRIMER_INTERNAL_178_HAIRPIN_TH=41.60 PRIMER_INTERNAL_179_PENALTY=8.463323 PRIMER_INTERNAL_179_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGATC PRIMER_INTERNAL_179=52,27 PRIMER_INTERNAL_179_TM=58.537 PRIMER_INTERNAL_179_GC_PERCENT=48.148 PRIMER_INTERNAL_179_SELF_ANY_TH=0.00 PRIMER_INTERNAL_179_SELF_END_TH=0.00 PRIMER_INTERNAL_179_HAIRPIN_TH=0.00 PRIMER_INTERNAL_180_PENALTY=8.486477 PRIMER_INTERNAL_180_SEQUENCE=CAGTAGACATGACACTGACGATGCAGA PRIMER_INTERNAL_180=6,27 PRIMER_INTERNAL_180_TM=58.514 PRIMER_INTERNAL_180_GC_PERCENT=48.148 PRIMER_INTERNAL_180_SELF_ANY_TH=12.05 PRIMER_INTERNAL_180_SELF_END_TH=0.00 PRIMER_INTERNAL_180_HAIRPIN_TH=0.00 PRIMER_INTERNAL_181_PENALTY=8.486477 PRIMER_INTERNAL_181_SEQUENCE=TCAGTAGACATGACACTGACGATGCAG PRIMER_INTERNAL_181=5,27 PRIMER_INTERNAL_181_TM=58.514 PRIMER_INTERNAL_181_GC_PERCENT=48.148 PRIMER_INTERNAL_181_SELF_ANY_TH=16.57 PRIMER_INTERNAL_181_SELF_END_TH=0.00 PRIMER_INTERNAL_181_HAIRPIN_TH=0.00 PRIMER_INTERNAL_182_PENALTY=8.487256 PRIMER_INTERNAL_182_SEQUENCE=AGTAGACATGACACTGACGATGCAGAC PRIMER_INTERNAL_182=7,27 PRIMER_INTERNAL_182_TM=58.513 PRIMER_INTERNAL_182_GC_PERCENT=48.148 PRIMER_INTERNAL_182_SELF_ANY_TH=1.44 PRIMER_INTERNAL_182_SELF_END_TH=0.00 PRIMER_INTERNAL_182_HAIRPIN_TH=0.00 PRIMER_INTERNAL_183_PENALTY=8.487256 PRIMER_INTERNAL_183_SEQUENCE=AGTCAGTAGACATGACACTGACGATGC PRIMER_INTERNAL_183=3,27 PRIMER_INTERNAL_183_TM=58.513 PRIMER_INTERNAL_183_GC_PERCENT=48.148 PRIMER_INTERNAL_183_SELF_ANY_TH=25.46 PRIMER_INTERNAL_183_SELF_END_TH=14.56 PRIMER_INTERNAL_183_HAIRPIN_TH=43.89 PRIMER_INTERNAL_184_PENALTY=8.514997 PRIMER_INTERNAL_184_SEQUENCE=ACACACACACAGCACACAGGTATTAGT PRIMER_INTERNAL_184=39,27 PRIMER_INTERNAL_184_TM=58.485 PRIMER_INTERNAL_184_GC_PERCENT=44.444 PRIMER_INTERNAL_184_SELF_ANY_TH=0.00 PRIMER_INTERNAL_184_SELF_END_TH=0.00 PRIMER_INTERNAL_184_HAIRPIN_TH=0.00 PRIMER_INTERNAL_185_PENALTY=8.547914 PRIMER_INTERNAL_185_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_185=59,27 PRIMER_INTERNAL_185_TM=61.548 PRIMER_INTERNAL_185_GC_PERCENT=55.556 PRIMER_INTERNAL_185_SELF_ANY_TH=5.61 PRIMER_INTERNAL_185_SELF_END_TH=2.93 PRIMER_INTERNAL_185_HAIRPIN_TH=41.60 PRIMER_INTERNAL_186_PENALTY=8.589885 PRIMER_INTERNAL_186_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATC PRIMER_INTERNAL_186=53,26 PRIMER_INTERNAL_186_TM=57.410 PRIMER_INTERNAL_186_GC_PERCENT=50.000 PRIMER_INTERNAL_186_SELF_ANY_TH=0.00 PRIMER_INTERNAL_186_SELF_END_TH=0.00 PRIMER_INTERNAL_186_HAIRPIN_TH=0.00 PRIMER_INTERNAL_187_PENALTY=8.642220 PRIMER_INTERNAL_187_SEQUENCE=ACACACACAGCACACAGGTATTAGTG PRIMER_INTERNAL_187=41,26 PRIMER_INTERNAL_187_TM=57.358 PRIMER_INTERNAL_187_GC_PERCENT=46.154 PRIMER_INTERNAL_187_SELF_ANY_TH=0.00 PRIMER_INTERNAL_187_SELF_END_TH=0.00 PRIMER_INTERNAL_187_HAIRPIN_TH=0.00 PRIMER_INTERNAL_188_PENALTY=8.642220 PRIMER_INTERNAL_188_SEQUENCE=CACACACACAGCACACAGGTATTAGT PRIMER_INTERNAL_188=40,26 PRIMER_INTERNAL_188_TM=57.358 PRIMER_INTERNAL_188_GC_PERCENT=46.154 PRIMER_INTERNAL_188_SELF_ANY_TH=0.00 PRIMER_INTERNAL_188_SELF_END_TH=0.00 PRIMER_INTERNAL_188_HAIRPIN_TH=0.00 PRIMER_INTERNAL_189_PENALTY=8.642220 PRIMER_INTERNAL_189_SEQUENCE=ACACACACACAGCACACAGGTATTAG PRIMER_INTERNAL_189=39,26 PRIMER_INTERNAL_189_TM=57.358 PRIMER_INTERNAL_189_GC_PERCENT=46.154 PRIMER_INTERNAL_189_SELF_ANY_TH=0.00 PRIMER_INTERNAL_189_SELF_END_TH=0.00 PRIMER_INTERNAL_189_HAIRPIN_TH=0.00 PRIMER_INTERNAL_190_PENALTY=8.687221 PRIMER_INTERNAL_190_SEQUENCE=TGGGCCATTCGATCCCGACCCAAATC PRIMER_INTERNAL_190=65,26 PRIMER_INTERNAL_190_TM=62.687 PRIMER_INTERNAL_190_GC_PERCENT=57.692 PRIMER_INTERNAL_190_SELF_ANY_TH=8.58 PRIMER_INTERNAL_190_SELF_END_TH=0.00 PRIMER_INTERNAL_190_HAIRPIN_TH=41.29 PRIMER_INTERNAL_191_PENALTY=8.713667 PRIMER_INTERNAL_191_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_191=67,26 PRIMER_INTERNAL_191_TM=62.714 PRIMER_INTERNAL_191_GC_PERCENT=57.692 PRIMER_INTERNAL_191_SELF_ANY_TH=0.00 PRIMER_INTERNAL_191_SELF_END_TH=0.00 PRIMER_INTERNAL_191_HAIRPIN_TH=41.60 PRIMER_INTERNAL_192_PENALTY=8.714713 PRIMER_INTERNAL_192_SEQUENCE=ACACAGCACACAGGTATTAGTGGGCCA PRIMER_INTERNAL_192=45,27 PRIMER_INTERNAL_192_TM=61.715 PRIMER_INTERNAL_192_GC_PERCENT=51.852 PRIMER_INTERNAL_192_SELF_ANY_TH=8.63 PRIMER_INTERNAL_192_SELF_END_TH=1.50 PRIMER_INTERNAL_192_HAIRPIN_TH=32.68 PRIMER_INTERNAL_193_PENALTY=8.787348 PRIMER_INTERNAL_193_SEQUENCE=CACACACACAGCACACAGGTATTAGTG PRIMER_INTERNAL_193=40,27 PRIMER_INTERNAL_193_TM=58.213 PRIMER_INTERNAL_193_GC_PERCENT=48.148 PRIMER_INTERNAL_193_SELF_ANY_TH=0.00 PRIMER_INTERNAL_193_SELF_END_TH=0.00 PRIMER_INTERNAL_193_HAIRPIN_TH=0.00 PRIMER_INTERNAL_194_PENALTY=8.787348 PRIMER_INTERNAL_194_SEQUENCE=CACACACACACAGCACACAGGTATTAG PRIMER_INTERNAL_194=38,27 PRIMER_INTERNAL_194_TM=58.213 PRIMER_INTERNAL_194_GC_PERCENT=48.148 PRIMER_INTERNAL_194_SELF_ANY_TH=0.00 PRIMER_INTERNAL_194_SELF_END_TH=0.00 PRIMER_INTERNAL_194_HAIRPIN_TH=0.00 PRIMER_INTERNAL_195_PENALTY=8.904972 PRIMER_INTERNAL_195_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAAT PRIMER_INTERNAL_195=64,26 PRIMER_INTERNAL_195_TM=62.905 PRIMER_INTERNAL_195_GC_PERCENT=57.692 PRIMER_INTERNAL_195_SELF_ANY_TH=8.58 PRIMER_INTERNAL_195_SELF_END_TH=0.00 PRIMER_INTERNAL_195_HAIRPIN_TH=43.79 PRIMER_INTERNAL_196_PENALTY=9.679371 PRIMER_INTERNAL_196_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_196=67,27 PRIMER_INTERNAL_196_TM=62.679 PRIMER_INTERNAL_196_GC_PERCENT=55.556 PRIMER_INTERNAL_196_SELF_ANY_TH=0.00 PRIMER_INTERNAL_196_SELF_END_TH=0.00 PRIMER_INTERNAL_196_HAIRPIN_TH=41.60 = PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_pick_primer_list_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33 PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33 PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=33 PRIMER_RIGHT_NUM_RETURNED=33 PRIMER_INTERNAL_NUM_RETURNED=73 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.486439 PRIMER_RIGHT_0_PENALTY=0.486439 PRIMER_INTERNAL_0_PENALTY=0.093814 PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA PRIMER_LEFT_0=118,20 PRIMER_RIGHT_0=137,20 PRIMER_INTERNAL_0=125,20 PRIMER_LEFT_0_TM=58.514 PRIMER_RIGHT_0_TM=58.514 PRIMER_INTERNAL_0_TM=59.906 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_SELF_ANY_TH=7.82 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=36.42 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon PRIMER_LEFT_0_END_STABILITY=5.3400 PRIMER_RIGHT_0_END_STABILITY=3.0100 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=8.2325 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0760 PRIMER_LEFT_1_PENALTY=1.160593 PRIMER_RIGHT_1_PENALTY=1.160593 PRIMER_INTERNAL_1_PENALTY=0.301069 PRIMER_LEFT_1_SEQUENCE=CAAAACGCAAAGCACGCTC PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTTG PRIMER_INTERNAL_1_SEQUENCE=ACGCAAAGCACGCTCCCGAT PRIMER_LEFT_1=122,19 PRIMER_RIGHT_1=140,19 PRIMER_INTERNAL_1=126,20 PRIMER_LEFT_1_TM=59.161 PRIMER_RIGHT_1_TM=59.161 PRIMER_INTERNAL_1_TM=59.699 PRIMER_LEFT_1_GC_PERCENT=52.632 PRIMER_RIGHT_1_GC_PERCENT=52.632 PRIMER_INTERNAL_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_SELF_ANY_TH=7.82 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=37.44 PRIMER_INTERNAL_1_HAIRPIN_TH=36.42 PRIMER_LEFT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_RIGHT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_1_END_STABILITY=5.0300 PRIMER_RIGHT_1_END_STABILITY=2.4400 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=5.7937 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=8.3885 PRIMER_LEFT_2_PENALTY=1.656085 PRIMER_RIGHT_2_PENALTY=1.656085 PRIMER_INTERNAL_2_PENALTY=0.900305 PRIMER_LEFT_2_SEQUENCE=TATTCCAAAACGCAAAGCACG PRIMER_RIGHT_2_SEQUENCE=CGTGCTTTGCGTTTTGGAATA PRIMER_INTERNAL_2_SEQUENCE=AAACGCAAAGCACGCTCCCG PRIMER_LEFT_2=117,21 PRIMER_RIGHT_2=137,21 PRIMER_INTERNAL_2=124,20 PRIMER_LEFT_2_TM=58.344 PRIMER_RIGHT_2_TM=58.344 PRIMER_INTERNAL_2_TM=59.100 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_SELF_ANY_TH=1.50 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=1.50 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=41.14 PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, reverse MER46 Nonautonomous DNA transposon PRIMER_LEFT_2_END_STABILITY=5.3400 PRIMER_RIGHT_2_END_STABILITY=1.7500 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=10.3450 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=9.4271 PRIMER_LEFT_3_PENALTY=1.695359 PRIMER_RIGHT_3_PENALTY=1.695359 PRIMER_INTERNAL_3_PENALTY=0.956520 PRIMER_LEFT_3_SEQUENCE=TGACCTGATAGAAACGCCAAC PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA PRIMER_INTERNAL_3_SEQUENCE=CGCAAAGCACGCTCCCGATC PRIMER_LEFT_3=53,21 PRIMER_RIGHT_3=73,21 PRIMER_INTERNAL_3=127,20 PRIMER_LEFT_3_TM=58.305 PRIMER_RIGHT_3_TM=58.305 PRIMER_INTERNAL_3_TM=59.043 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=36.42 PRIMER_LEFT_3_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene PRIMER_RIGHT_3_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=3.7700 PRIMER_RIGHT_3_END_STABILITY=4.0200 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=6.6359 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.3924 PRIMER_LEFT_4_PENALTY=1.831685 PRIMER_RIGHT_4_PENALTY=1.831685 PRIMER_INTERNAL_4_PENALTY=1.018091 PRIMER_LEFT_4_SEQUENCE=AACGCCAACTCACTGTATGA PRIMER_RIGHT_4_SEQUENCE=TCATACAGTGAGTTGGCGTT PRIMER_INTERNAL_4_SEQUENCE=AACGCAAAGCACGCTCCCGAT PRIMER_LEFT_4=65,20 PRIMER_RIGHT_4=84,20 PRIMER_INTERNAL_4=125,21 PRIMER_LEFT_4_TM=57.168 PRIMER_RIGHT_4_TM=57.168 PRIMER_INTERNAL_4_TM=59.982 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=57.143 PRIMER_INTERNAL_4_SELF_ANY_TH=7.82 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=35.45 PRIMER_RIGHT_4_HAIRPIN_TH=31.80 PRIMER_INTERNAL_4_HAIRPIN_TH=36.42 PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_RIGHT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_LEFT_4_END_STABILITY=2.1500 PRIMER_RIGHT_4_END_STABILITY=4.8400 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=18.7112 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=25.7856 PRIMER_LEFT_5_PENALTY=1.902705 PRIMER_RIGHT_5_PENALTY=1.902705 PRIMER_INTERNAL_5_PENALTY=1.178696 PRIMER_LEFT_5_SEQUENCE=AGAAACGCCAACTCACTGTA PRIMER_RIGHT_5_SEQUENCE=TACAGTGAGTTGGCGTTTCT PRIMER_INTERNAL_5_SEQUENCE=AAACGCAAAGCACGCTCCCGA PRIMER_LEFT_5=62,20 PRIMER_RIGHT_5=81,20 PRIMER_INTERNAL_5=124,21 PRIMER_LEFT_5_TM=57.097 PRIMER_RIGHT_5_TM=57.097 PRIMER_INTERNAL_5_TM=60.179 PRIMER_LEFT_5_GC_PERCENT=45.000 PRIMER_RIGHT_5_GC_PERCENT=45.000 PRIMER_INTERNAL_5_GC_PERCENT=57.143 PRIMER_INTERNAL_5_SELF_ANY_TH=7.82 PRIMER_LEFT_5_SELF_ANY_TH=0.00 PRIMER_RIGHT_5_SELF_ANY_TH=0.00 PRIMER_INTERNAL_5_SELF_END_TH=0.00 PRIMER_LEFT_5_SELF_END_TH=0.00 PRIMER_RIGHT_5_SELF_END_TH=0.00 PRIMER_LEFT_5_HAIRPIN_TH=0.00 PRIMER_RIGHT_5_HAIRPIN_TH=0.00 PRIMER_INTERNAL_5_HAIRPIN_TH=36.42 PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_5_END_STABILITY=2.7400 PRIMER_RIGHT_5_END_STABILITY=2.5200 PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=15.5573 PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=19.2811 PRIMER_LEFT_6_PENALTY=1.902705 PRIMER_RIGHT_6_PENALTY=1.902705 PRIMER_INTERNAL_6_PENALTY=1.372876 PRIMER_LEFT_6_SEQUENCE=TAGAAACGCCAACTCACTGT PRIMER_RIGHT_6_SEQUENCE=ACAGTGAGTTGGCGTTTCTA PRIMER_INTERNAL_6_SEQUENCE=ACGCAAAGCACGCTCCCGATC PRIMER_LEFT_6=61,20 PRIMER_RIGHT_6=80,20 PRIMER_INTERNAL_6=126,21 PRIMER_LEFT_6_TM=57.097 PRIMER_RIGHT_6_TM=57.097 PRIMER_INTERNAL_6_TM=60.373 PRIMER_LEFT_6_GC_PERCENT=45.000 PRIMER_RIGHT_6_GC_PERCENT=45.000 PRIMER_INTERNAL_6_GC_PERCENT=61.905 PRIMER_INTERNAL_6_SELF_ANY_TH=7.82 PRIMER_LEFT_6_SELF_ANY_TH=0.00 PRIMER_RIGHT_6_SELF_ANY_TH=0.00 PRIMER_INTERNAL_6_SELF_END_TH=0.00 PRIMER_LEFT_6_SELF_END_TH=0.00 PRIMER_RIGHT_6_SELF_END_TH=0.00 PRIMER_LEFT_6_HAIRPIN_TH=0.00 PRIMER_RIGHT_6_HAIRPIN_TH=0.00 PRIMER_INTERNAL_6_HAIRPIN_TH=36.42 PRIMER_LEFT_6_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_6_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_6_END_STABILITY=3.5500 PRIMER_RIGHT_6_END_STABILITY=2.1000 PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=10.6833 PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=20.2263 PRIMER_LEFT_7_PENALTY=1.950877 PRIMER_RIGHT_7_PENALTY=1.950877 PRIMER_INTERNAL_7_PENALTY=1.393730 PRIMER_LEFT_7_SEQUENCE=TTCCAAAACGCAAAGCACG PRIMER_RIGHT_7_SEQUENCE=CGTGCTTTGCGTTTTGGAA PRIMER_INTERNAL_7_SEQUENCE=ACGCAAAGCACGCTCCCGA PRIMER_LEFT_7=119,19 PRIMER_RIGHT_7=137,19 PRIMER_INTERNAL_7=126,19 PRIMER_LEFT_7_TM=58.049 PRIMER_RIGHT_7_TM=58.049 PRIMER_INTERNAL_7_TM=59.606 PRIMER_LEFT_7_GC_PERCENT=47.368 PRIMER_RIGHT_7_GC_PERCENT=47.368 PRIMER_INTERNAL_7_GC_PERCENT=63.158 PRIMER_INTERNAL_7_SELF_ANY_TH=7.82 PRIMER_LEFT_7_SELF_ANY_TH=0.00 PRIMER_RIGHT_7_SELF_ANY_TH=0.00 PRIMER_INTERNAL_7_SELF_END_TH=0.00 PRIMER_LEFT_7_SELF_END_TH=0.00 PRIMER_RIGHT_7_SELF_END_TH=0.00 PRIMER_LEFT_7_HAIRPIN_TH=0.00 PRIMER_RIGHT_7_HAIRPIN_TH=0.00 PRIMER_INTERNAL_7_HAIRPIN_TH=36.42 PRIMER_LEFT_7_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_7_END_STABILITY=5.3400 PRIMER_RIGHT_7_END_STABILITY=3.5300 PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=2.1013 PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=4.8280 PRIMER_LEFT_8_PENALTY=1.972625 PRIMER_RIGHT_8_PENALTY=1.972625 PRIMER_INTERNAL_8_PENALTY=1.595076 PRIMER_LEFT_8_SEQUENCE=CCTGATAGAAACGCCAACTCA PRIMER_RIGHT_8_SEQUENCE=TGAGTTGGCGTTTCTATCAGG PRIMER_INTERNAL_8_SEQUENCE=AAAACGCAAAGCACGCTCCCG PRIMER_LEFT_8=56,21 PRIMER_RIGHT_8=76,21 PRIMER_INTERNAL_8=123,21 PRIMER_LEFT_8_TM=58.027 PRIMER_RIGHT_8_TM=58.027 PRIMER_INTERNAL_8_TM=59.405 PRIMER_LEFT_8_GC_PERCENT=47.619 PRIMER_RIGHT_8_GC_PERCENT=47.619 PRIMER_INTERNAL_8_GC_PERCENT=57.143 PRIMER_INTERNAL_8_SELF_ANY_TH=1.50 PRIMER_LEFT_8_SELF_ANY_TH=0.00 PRIMER_RIGHT_8_SELF_ANY_TH=0.00 PRIMER_INTERNAL_8_SELF_END_TH=1.50 PRIMER_LEFT_8_SELF_END_TH=0.00 PRIMER_RIGHT_8_SELF_END_TH=0.00 PRIMER_LEFT_8_HAIRPIN_TH=0.00 PRIMER_RIGHT_8_HAIRPIN_TH=0.00 PRIMER_INTERNAL_8_HAIRPIN_TH=41.14 PRIMER_LEFT_8_LIBRARY_MISPRIMING=10.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1) PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene PRIMER_LEFT_8_END_STABILITY=3.4100 PRIMER_RIGHT_8_END_STABILITY=3.8600 PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=20.8283 PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=12.8893 PRIMER_LEFT_9_PENALTY=1.973945 PRIMER_RIGHT_9_PENALTY=1.973945 PRIMER_INTERNAL_9_PENALTY=2.007622 PRIMER_LEFT_9_SEQUENCE=ACCTGATAGAAACGCCAACTC PRIMER_RIGHT_9_SEQUENCE=GAGTTGGCGTTTCTATCAGGT PRIMER_INTERNAL_9_SEQUENCE=CCAAAACGCAAAGCACGCTCCC PRIMER_LEFT_9=55,21 PRIMER_RIGHT_9=75,21 PRIMER_INTERNAL_9=121,22 PRIMER_LEFT_9_TM=58.026 PRIMER_RIGHT_9_TM=58.026 PRIMER_INTERNAL_9_TM=59.992 PRIMER_LEFT_9_GC_PERCENT=47.619 PRIMER_RIGHT_9_GC_PERCENT=47.619 PRIMER_INTERNAL_9_GC_PERCENT=59.091 PRIMER_INTERNAL_9_SELF_ANY_TH=0.00 PRIMER_LEFT_9_SELF_ANY_TH=0.00 PRIMER_RIGHT_9_SELF_ANY_TH=0.00 PRIMER_INTERNAL_9_SELF_END_TH=0.00 PRIMER_LEFT_9_SELF_END_TH=0.00 PRIMER_RIGHT_9_SELF_END_TH=0.00 PRIMER_LEFT_9_HAIRPIN_TH=0.00 PRIMER_RIGHT_9_HAIRPIN_TH=0.00 PRIMER_INTERNAL_9_HAIRPIN_TH=0.00 PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_9_END_STABILITY=3.0100 PRIMER_RIGHT_9_END_STABILITY=4.0000 PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=6.8289 PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=11.0226 PRIMER_LEFT_10_PENALTY=1.973945 PRIMER_RIGHT_10_PENALTY=1.973945 PRIMER_INTERNAL_10_PENALTY=2.235780 PRIMER_LEFT_10_SEQUENCE=GACCTGATAGAAACGCCAACT PRIMER_RIGHT_10_SEQUENCE=AGTTGGCGTTTCTATCAGGTC PRIMER_INTERNAL_10_SEQUENCE=AACGCAAAGCACGCTCCCG PRIMER_LEFT_10=54,21 PRIMER_RIGHT_10=74,21 PRIMER_INTERNAL_10=125,19 PRIMER_LEFT_10_TM=58.026 PRIMER_RIGHT_10_TM=58.026 PRIMER_INTERNAL_10_TM=58.764 PRIMER_LEFT_10_GC_PERCENT=47.619 PRIMER_RIGHT_10_GC_PERCENT=47.619 PRIMER_INTERNAL_10_GC_PERCENT=63.158 PRIMER_INTERNAL_10_SELF_ANY_TH=1.50 PRIMER_LEFT_10_SELF_ANY_TH=0.00 PRIMER_RIGHT_10_SELF_ANY_TH=0.00 PRIMER_INTERNAL_10_SELF_END_TH=1.50 PRIMER_LEFT_10_SELF_END_TH=0.00 PRIMER_RIGHT_10_SELF_END_TH=0.00 PRIMER_LEFT_10_HAIRPIN_TH=0.00 PRIMER_RIGHT_10_HAIRPIN_TH=0.00 PRIMER_INTERNAL_10_HAIRPIN_TH=41.14 PRIMER_LEFT_10_LIBRARY_MISPRIMING=10.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_10_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_10_END_STABILITY=3.1600 PRIMER_RIGHT_10_END_STABILITY=3.8500 PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=3.2290 PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=8.6567 PRIMER_LEFT_11_PENALTY=2.120321 PRIMER_RIGHT_11_PENALTY=2.120321 PRIMER_INTERNAL_11_PENALTY=2.240074 PRIMER_LEFT_11_SEQUENCE=AAACGCCAACTCACTGTATGA PRIMER_RIGHT_11_SEQUENCE=TCATACAGTGAGTTGGCGTTT PRIMER_INTERNAL_11_SEQUENCE=AAACGCAAAGCACGCTCCCGAT PRIMER_LEFT_11=64,21 PRIMER_RIGHT_11=84,21 PRIMER_INTERNAL_11=124,22 PRIMER_LEFT_11_TM=57.880 PRIMER_RIGHT_11_TM=57.880 PRIMER_INTERNAL_11_TM=60.240 PRIMER_LEFT_11_GC_PERCENT=42.857 PRIMER_RIGHT_11_GC_PERCENT=42.857 PRIMER_INTERNAL_11_GC_PERCENT=54.545 PRIMER_INTERNAL_11_SELF_ANY_TH=7.82 PRIMER_LEFT_11_SELF_ANY_TH=0.00 PRIMER_RIGHT_11_SELF_ANY_TH=0.00 PRIMER_INTERNAL_11_SELF_END_TH=0.00 PRIMER_LEFT_11_SELF_END_TH=0.00 PRIMER_RIGHT_11_SELF_END_TH=0.00 PRIMER_LEFT_11_HAIRPIN_TH=35.45 PRIMER_RIGHT_11_HAIRPIN_TH=31.80 PRIMER_INTERNAL_11_HAIRPIN_TH=36.42 PRIMER_LEFT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_LEFT_11_END_STABILITY=2.1500 PRIMER_RIGHT_11_END_STABILITY=3.6000 PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=5.5045 PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=24.8608 PRIMER_LEFT_12_PENALTY=2.142778 PRIMER_RIGHT_12_PENALTY=2.142778 PRIMER_INTERNAL_12_PENALTY=2.299276 PRIMER_LEFT_12_SEQUENCE=TTCCAAAACGCAAAGCACGC PRIMER_RIGHT_12_SEQUENCE=GCGTGCTTTGCGTTTTGGAA PRIMER_INTERNAL_12_SEQUENCE=CAAAACGCAAAGCACGCTCCCG PRIMER_LEFT_12=119,20 PRIMER_RIGHT_12=138,20 PRIMER_INTERNAL_12=122,22 PRIMER_LEFT_12_TM=61.143 PRIMER_RIGHT_12_TM=61.143 PRIMER_INTERNAL_12_TM=60.299 PRIMER_LEFT_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_GC_PERCENT=50.000 PRIMER_INTERNAL_12_GC_PERCENT=59.091 PRIMER_INTERNAL_12_SELF_ANY_TH=1.50 PRIMER_LEFT_12_SELF_ANY_TH=0.00 PRIMER_RIGHT_12_SELF_ANY_TH=0.00 PRIMER_INTERNAL_12_SELF_END_TH=1.50 PRIMER_LEFT_12_SELF_END_TH=0.00 PRIMER_RIGHT_12_SELF_END_TH=0.00 PRIMER_LEFT_12_HAIRPIN_TH=0.00 PRIMER_RIGHT_12_HAIRPIN_TH=0.00 PRIMER_INTERNAL_12_HAIRPIN_TH=41.14 PRIMER_LEFT_12_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner PRIMER_RIGHT_12_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_12_END_STABILITY=5.3400 PRIMER_RIGHT_12_END_STABILITY=3.5300 PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=6.5778 PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=7.7746 PRIMER_LEFT_13_PENALTY=2.299089 PRIMER_RIGHT_13_PENALTY=2.299089 PRIMER_INTERNAL_13_PENALTY=2.427391 PRIMER_LEFT_13_SEQUENCE=AAAACGCAAAGCACGCTCC PRIMER_RIGHT_13_SEQUENCE=GGAGCGTGCTTTGCGTTTT PRIMER_INTERNAL_13_SEQUENCE=AAAACGCAAAGCACGCTCCCGA PRIMER_LEFT_13=123,19 PRIMER_RIGHT_13=141,19 PRIMER_INTERNAL_13=123,22 PRIMER_LEFT_13_TM=60.299 PRIMER_RIGHT_13_TM=60.299 PRIMER_INTERNAL_13_TM=60.427 PRIMER_LEFT_13_GC_PERCENT=52.632 PRIMER_RIGHT_13_GC_PERCENT=52.632 PRIMER_INTERNAL_13_GC_PERCENT=54.545 PRIMER_INTERNAL_13_SELF_ANY_TH=7.82 PRIMER_LEFT_13_SELF_ANY_TH=0.00 PRIMER_RIGHT_13_SELF_ANY_TH=0.00 PRIMER_INTERNAL_13_SELF_END_TH=0.00 PRIMER_LEFT_13_SELF_END_TH=0.00 PRIMER_RIGHT_13_SELF_END_TH=0.00 PRIMER_LEFT_13_HAIRPIN_TH=0.00 PRIMER_RIGHT_13_HAIRPIN_TH=37.44 PRIMER_INTERNAL_13_HAIRPIN_TH=36.42 PRIMER_LEFT_13_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_13_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_13_END_STABILITY=4.7000 PRIMER_RIGHT_13_END_STABILITY=2.4300 PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=0.4266 PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=8.7659 PRIMER_LEFT_14_PENALTY=2.402628 PRIMER_RIGHT_14_PENALTY=2.402628 PRIMER_INTERNAL_14_PENALTY=2.610811 PRIMER_LEFT_14_SEQUENCE=AGAAACGCCAACTCACTGTAT PRIMER_RIGHT_14_SEQUENCE=ATACAGTGAGTTGGCGTTTCT PRIMER_INTERNAL_14_SEQUENCE=AACGCAAAGCACGCTCCCGATC PRIMER_LEFT_14=62,21 PRIMER_RIGHT_14=82,21 PRIMER_INTERNAL_14=125,22 PRIMER_LEFT_14_TM=57.597 PRIMER_RIGHT_14_TM=57.597 PRIMER_INTERNAL_14_TM=60.611 PRIMER_LEFT_14_GC_PERCENT=42.857 PRIMER_RIGHT_14_GC_PERCENT=42.857 PRIMER_INTERNAL_14_GC_PERCENT=59.091 PRIMER_INTERNAL_14_SELF_ANY_TH=7.82 PRIMER_LEFT_14_SELF_ANY_TH=0.00 PRIMER_RIGHT_14_SELF_ANY_TH=0.00 PRIMER_INTERNAL_14_SELF_END_TH=0.00 PRIMER_LEFT_14_SELF_END_TH=0.00 PRIMER_RIGHT_14_SELF_END_TH=0.00 PRIMER_LEFT_14_HAIRPIN_TH=0.00 PRIMER_RIGHT_14_HAIRPIN_TH=0.00 PRIMER_INTERNAL_14_HAIRPIN_TH=36.42 PRIMER_LEFT_14_LIBRARY_MISPRIMING=11.00, reverse MARINER1 Human mariner PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_14_END_STABILITY=2.2900 PRIMER_RIGHT_14_END_STABILITY=2.5200 PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=16.5430 PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=19.2811 PRIMER_LEFT_15_PENALTY=2.402628 PRIMER_RIGHT_15_PENALTY=2.402628 PRIMER_INTERNAL_15_PENALTY=2.740095 PRIMER_LEFT_15_SEQUENCE=ATAGAAACGCCAACTCACTGT PRIMER_RIGHT_15_SEQUENCE=ACAGTGAGTTGGCGTTTCTAT PRIMER_INTERNAL_15_SEQUENCE=CGCAAAGCACGCTCCCGAT PRIMER_LEFT_15=60,21 PRIMER_RIGHT_15=80,21 PRIMER_INTERNAL_15=127,19 PRIMER_LEFT_15_TM=57.597 PRIMER_RIGHT_15_TM=57.597 PRIMER_INTERNAL_15_TM=58.260 PRIMER_LEFT_15_GC_PERCENT=42.857 PRIMER_RIGHT_15_GC_PERCENT=42.857 PRIMER_INTERNAL_15_GC_PERCENT=63.158 PRIMER_INTERNAL_15_SELF_ANY_TH=0.00 PRIMER_LEFT_15_SELF_ANY_TH=0.00 PRIMER_RIGHT_15_SELF_ANY_TH=0.00 PRIMER_INTERNAL_15_SELF_END_TH=0.00 PRIMER_LEFT_15_SELF_END_TH=0.00 PRIMER_RIGHT_15_SELF_END_TH=0.00 PRIMER_LEFT_15_HAIRPIN_TH=0.00 PRIMER_RIGHT_15_HAIRPIN_TH=0.00 PRIMER_INTERNAL_15_HAIRPIN_TH=36.42 PRIMER_LEFT_15_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_15_END_STABILITY=3.5500 PRIMER_RIGHT_15_END_STABILITY=1.9800 PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=14.5010 PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=21.0214 PRIMER_LEFT_16_PENALTY=2.455619 PRIMER_RIGHT_16_PENALTY=2.455619 PRIMER_INTERNAL_16_PENALTY=2.880381 PRIMER_LEFT_16_SEQUENCE=GAAACGCCAACTCACTGTATG PRIMER_RIGHT_16_SEQUENCE=CATACAGTGAGTTGGCGTTTC PRIMER_INTERNAL_16_SEQUENCE=TCCAAAACGCAAAGCACGCTCC PRIMER_LEFT_16=63,21 PRIMER_RIGHT_16=83,21 PRIMER_INTERNAL_16=120,22 PRIMER_LEFT_16_TM=57.544 PRIMER_RIGHT_16_TM=57.544 PRIMER_INTERNAL_16_TM=59.120 PRIMER_LEFT_16_GC_PERCENT=47.619 PRIMER_RIGHT_16_GC_PERCENT=47.619 PRIMER_INTERNAL_16_GC_PERCENT=54.545 PRIMER_INTERNAL_16_SELF_ANY_TH=0.00 PRIMER_LEFT_16_SELF_ANY_TH=0.00 PRIMER_RIGHT_16_SELF_ANY_TH=0.00 PRIMER_INTERNAL_16_SELF_END_TH=0.00 PRIMER_LEFT_16_SELF_END_TH=0.00 PRIMER_RIGHT_16_SELF_END_TH=0.00 PRIMER_LEFT_16_HAIRPIN_TH=0.00 PRIMER_RIGHT_16_HAIRPIN_TH=0.00 PRIMER_INTERNAL_16_HAIRPIN_TH=0.00 PRIMER_LEFT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner PRIMER_RIGHT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner PRIMER_LEFT_16_END_STABILITY=2.3900 PRIMER_RIGHT_16_END_STABILITY=2.7800 PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=9.1798 PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=18.8273 PRIMER_LEFT_17_PENALTY=2.539776 PRIMER_RIGHT_17_PENALTY=2.539776 PRIMER_INTERNAL_17_PENALTY=2.939429 PRIMER_LEFT_17_SEQUENCE=CAAAACGCAAAGCACGCTCC PRIMER_RIGHT_17_SEQUENCE=GGAGCGTGCTTTGCGTTTTG PRIMER_INTERNAL_17_SEQUENCE=CAAAACGCAAAGCACGCTCCC PRIMER_LEFT_17=122,20 PRIMER_RIGHT_17=141,20 PRIMER_INTERNAL_17=122,21 PRIMER_LEFT_17_TM=61.540 PRIMER_RIGHT_17_TM=61.540 PRIMER_INTERNAL_17_TM=58.061 PRIMER_LEFT_17_GC_PERCENT=55.000 PRIMER_RIGHT_17_GC_PERCENT=55.000 PRIMER_INTERNAL_17_GC_PERCENT=57.143 PRIMER_INTERNAL_17_SELF_ANY_TH=0.00 PRIMER_LEFT_17_SELF_ANY_TH=0.00 PRIMER_RIGHT_17_SELF_ANY_TH=0.00 PRIMER_INTERNAL_17_SELF_END_TH=0.00 PRIMER_LEFT_17_SELF_END_TH=0.00 PRIMER_RIGHT_17_SELF_END_TH=0.00 PRIMER_LEFT_17_HAIRPIN_TH=0.00 PRIMER_RIGHT_17_HAIRPIN_TH=37.44 PRIMER_INTERNAL_17_HAIRPIN_TH=0.00 PRIMER_LEFT_17_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_17_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_17_END_STABILITY=4.7000 PRIMER_RIGHT_17_END_STABILITY=2.4400 PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=4.7389 PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=6.1004 PRIMER_LEFT_18_PENALTY=2.539776 PRIMER_RIGHT_18_PENALTY=2.539776 PRIMER_INTERNAL_18_PENALTY=2.939429 PRIMER_LEFT_18_SEQUENCE=CCAAAACGCAAAGCACGCTC PRIMER_RIGHT_18_SEQUENCE=GAGCGTGCTTTGCGTTTTGG PRIMER_INTERNAL_18_SEQUENCE=CCAAAACGCAAAGCACGCTCC PRIMER_LEFT_18=121,20 PRIMER_RIGHT_18=140,20 PRIMER_INTERNAL_18=121,21 PRIMER_LEFT_18_TM=61.540 PRIMER_RIGHT_18_TM=61.540 PRIMER_INTERNAL_18_TM=58.061 PRIMER_LEFT_18_GC_PERCENT=55.000 PRIMER_RIGHT_18_GC_PERCENT=55.000 PRIMER_INTERNAL_18_GC_PERCENT=57.143 PRIMER_INTERNAL_18_SELF_ANY_TH=0.00 PRIMER_LEFT_18_SELF_ANY_TH=0.00 PRIMER_RIGHT_18_SELF_ANY_TH=0.00 PRIMER_INTERNAL_18_SELF_END_TH=0.00 PRIMER_LEFT_18_SELF_END_TH=0.00 PRIMER_RIGHT_18_SELF_END_TH=0.00 PRIMER_LEFT_18_HAIRPIN_TH=0.00 PRIMER_RIGHT_18_HAIRPIN_TH=37.44 PRIMER_INTERNAL_18_HAIRPIN_TH=0.00 PRIMER_LEFT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_RIGHT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_18_END_STABILITY=5.0300 PRIMER_RIGHT_18_END_STABILITY=3.2800 PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=7.7786 PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=9.7248 PRIMER_LEFT_19_PENALTY=2.587613 PRIMER_RIGHT_19_PENALTY=2.587613 PRIMER_INTERNAL_19_PENALTY=3.476553 PRIMER_LEFT_19_SEQUENCE=TCCAAAACGCAAAGCACGC PRIMER_RIGHT_19_SEQUENCE=GCGTGCTTTGCGTTTTGGA PRIMER_INTERNAL_19_SEQUENCE=AAAACGCAAAGCACGCTCCCGAT PRIMER_LEFT_19=120,19 PRIMER_RIGHT_19=138,19 PRIMER_INTERNAL_19=123,23 PRIMER_LEFT_19_TM=60.588 PRIMER_RIGHT_19_TM=60.588 PRIMER_INTERNAL_19_TM=60.477 PRIMER_LEFT_19_GC_PERCENT=52.632 PRIMER_RIGHT_19_GC_PERCENT=52.632 PRIMER_INTERNAL_19_GC_PERCENT=52.174 PRIMER_INTERNAL_19_SELF_ANY_TH=7.82 PRIMER_LEFT_19_SELF_ANY_TH=0.00 PRIMER_RIGHT_19_SELF_ANY_TH=0.00 PRIMER_INTERNAL_19_SELF_END_TH=0.00 PRIMER_LEFT_19_SELF_END_TH=0.00 PRIMER_RIGHT_19_SELF_END_TH=0.00 PRIMER_LEFT_19_HAIRPIN_TH=0.00 PRIMER_RIGHT_19_HAIRPIN_TH=0.00 PRIMER_INTERNAL_19_HAIRPIN_TH=36.42 PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner PRIMER_RIGHT_19_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_19_END_STABILITY=5.3400 PRIMER_RIGHT_19_END_STABILITY=3.5300 PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=6.3533 PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=6.8599 PRIMER_LEFT_20_PENALTY=2.588667 PRIMER_RIGHT_20_PENALTY=2.588667 PRIMER_INTERNAL_20_PENALTY=3.598972 PRIMER_LEFT_20_SEQUENCE=CCAAAACGCAAAGCACGCT PRIMER_RIGHT_20_SEQUENCE=AGCGTGCTTTGCGTTTTGG PRIMER_INTERNAL_20_SEQUENCE=TTCCAAAACGCAAAGCACGCTCC PRIMER_LEFT_20=121,19 PRIMER_RIGHT_20=139,19 PRIMER_INTERNAL_20=119,23 PRIMER_LEFT_20_TM=60.589 PRIMER_RIGHT_20_TM=60.589 PRIMER_INTERNAL_20_TM=59.401 PRIMER_LEFT_20_GC_PERCENT=52.632 PRIMER_RIGHT_20_GC_PERCENT=52.632 PRIMER_INTERNAL_20_GC_PERCENT=52.174 PRIMER_INTERNAL_20_SELF_ANY_TH=0.00 PRIMER_LEFT_20_SELF_ANY_TH=0.00 PRIMER_RIGHT_20_SELF_ANY_TH=0.00 PRIMER_INTERNAL_20_SELF_END_TH=0.00 PRIMER_LEFT_20_SELF_END_TH=0.00 PRIMER_RIGHT_20_SELF_END_TH=0.00 PRIMER_LEFT_20_HAIRPIN_TH=0.00 PRIMER_RIGHT_20_HAIRPIN_TH=37.44 PRIMER_INTERNAL_20_HAIRPIN_TH=0.00 PRIMER_LEFT_20_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_20_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner PRIMER_LEFT_20_END_STABILITY=5.0700 PRIMER_RIGHT_20_END_STABILITY=3.2800 PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=7.2070 PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=12.0561 PRIMER_LEFT_21_PENALTY=2.627395 PRIMER_RIGHT_21_PENALTY=2.627395 PRIMER_INTERNAL_21_PENALTY=3.606233 PRIMER_LEFT_21_SEQUENCE=ATGACCTGATAGAAACGCCAA PRIMER_RIGHT_21_SEQUENCE=TTGGCGTTTCTATCAGGTCAT PRIMER_INTERNAL_21_SEQUENCE=ACGCAAAGCACGCTCCCG PRIMER_LEFT_21=52,21 PRIMER_RIGHT_21=72,21 PRIMER_INTERNAL_21=126,18 PRIMER_LEFT_21_TM=57.373 PRIMER_RIGHT_21_TM=57.373 PRIMER_INTERNAL_21_TM=58.394 PRIMER_LEFT_21_GC_PERCENT=42.857 PRIMER_RIGHT_21_GC_PERCENT=42.857 PRIMER_INTERNAL_21_GC_PERCENT=66.667 PRIMER_INTERNAL_21_SELF_ANY_TH=1.50 PRIMER_LEFT_21_SELF_ANY_TH=0.00 PRIMER_RIGHT_21_SELF_ANY_TH=0.00 PRIMER_INTERNAL_21_SELF_END_TH=1.50 PRIMER_LEFT_21_SELF_END_TH=0.00 PRIMER_RIGHT_21_SELF_END_TH=0.00 PRIMER_LEFT_21_HAIRPIN_TH=0.00 PRIMER_RIGHT_21_HAIRPIN_TH=0.00 PRIMER_INTERNAL_21_HAIRPIN_TH=41.14 PRIMER_LEFT_21_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_21_END_STABILITY=4.5200 PRIMER_RIGHT_21_END_STABILITY=3.0600 PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=3.5472 PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=0.2868 PRIMER_LEFT_22_PENALTY=2.678056 PRIMER_RIGHT_22_PENALTY=2.678056 PRIMER_INTERNAL_22_PENALTY=3.829005 PRIMER_LEFT_22_SEQUENCE=TGATAGAAACGCCAACTCACT PRIMER_RIGHT_22_SEQUENCE=AGTGAGTTGGCGTTTCTATCA PRIMER_INTERNAL_22_SEQUENCE=AAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_22=58,21 PRIMER_RIGHT_22=78,21 PRIMER_INTERNAL_22=124,23 PRIMER_LEFT_22_TM=57.322 PRIMER_RIGHT_22_TM=57.322 PRIMER_INTERNAL_22_TM=60.829 PRIMER_LEFT_22_GC_PERCENT=42.857 PRIMER_RIGHT_22_GC_PERCENT=42.857 PRIMER_INTERNAL_22_GC_PERCENT=56.522 PRIMER_INTERNAL_22_SELF_ANY_TH=7.82 PRIMER_LEFT_22_SELF_ANY_TH=0.00 PRIMER_RIGHT_22_SELF_ANY_TH=0.00 PRIMER_INTERNAL_22_SELF_END_TH=0.00 PRIMER_LEFT_22_SELF_END_TH=0.00 PRIMER_RIGHT_22_SELF_END_TH=0.00 PRIMER_LEFT_22_HAIRPIN_TH=0.00 PRIMER_RIGHT_22_HAIRPIN_TH=0.00 PRIMER_INTERNAL_22_HAIRPIN_TH=36.42 PRIMER_LEFT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_22_END_STABILITY=3.4100 PRIMER_RIGHT_22_END_STABILITY=2.1500 PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=16.4194 PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=13.4901 PRIMER_LEFT_23_PENALTY=2.780624 PRIMER_RIGHT_23_PENALTY=2.780624 PRIMER_INTERNAL_23_PENALTY=3.865064 PRIMER_LEFT_23_SEQUENCE=AGAAACGCCAACTCACTGT PRIMER_RIGHT_23_SEQUENCE=ACAGTGAGTTGGCGTTTCT PRIMER_INTERNAL_23_SEQUENCE=TCCAAAACGCAAAGCACGCTC PRIMER_LEFT_23=62,19 PRIMER_RIGHT_23=80,19 PRIMER_INTERNAL_23=120,21 PRIMER_LEFT_23_TM=57.219 PRIMER_RIGHT_23_TM=57.219 PRIMER_INTERNAL_23_TM=57.135 PRIMER_LEFT_23_GC_PERCENT=47.368 PRIMER_RIGHT_23_GC_PERCENT=47.368 PRIMER_INTERNAL_23_GC_PERCENT=52.381 PRIMER_INTERNAL_23_SELF_ANY_TH=0.00 PRIMER_LEFT_23_SELF_ANY_TH=0.00 PRIMER_RIGHT_23_SELF_ANY_TH=0.00 PRIMER_INTERNAL_23_SELF_END_TH=0.00 PRIMER_LEFT_23_SELF_END_TH=0.00 PRIMER_RIGHT_23_SELF_END_TH=0.00 PRIMER_LEFT_23_HAIRPIN_TH=0.00 PRIMER_RIGHT_23_HAIRPIN_TH=0.00 PRIMER_INTERNAL_23_HAIRPIN_TH=0.00 PRIMER_LEFT_23_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_23_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_23_END_STABILITY=3.5500 PRIMER_RIGHT_23_END_STABILITY=2.5200 PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=8.5732 PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=19.2811 PRIMER_LEFT_24_PENALTY=2.999071 PRIMER_RIGHT_24_PENALTY=2.999071 PRIMER_INTERNAL_24_PENALTY=3.905453 PRIMER_LEFT_24_SEQUENCE=TCCAAAACGCAAAGCACGCT PRIMER_RIGHT_24_SEQUENCE=AGCGTGCTTTGCGTTTTGGA PRIMER_INTERNAL_24_SEQUENCE=CGCAAAGCACGCTCCCGA PRIMER_LEFT_24=120,20 PRIMER_RIGHT_24=139,20 PRIMER_INTERNAL_24=127,18 PRIMER_LEFT_24_TM=61.999 PRIMER_RIGHT_24_TM=61.999 PRIMER_INTERNAL_24_TM=58.095 PRIMER_LEFT_24_GC_PERCENT=50.000 PRIMER_RIGHT_24_GC_PERCENT=50.000 PRIMER_INTERNAL_24_GC_PERCENT=66.667 PRIMER_INTERNAL_24_SELF_ANY_TH=0.00 PRIMER_LEFT_24_SELF_ANY_TH=0.00 PRIMER_RIGHT_24_SELF_ANY_TH=0.00 PRIMER_INTERNAL_24_SELF_END_TH=0.00 PRIMER_LEFT_24_SELF_END_TH=0.00 PRIMER_RIGHT_24_SELF_END_TH=0.00 PRIMER_LEFT_24_HAIRPIN_TH=0.00 PRIMER_RIGHT_24_HAIRPIN_TH=37.44 PRIMER_INTERNAL_24_HAIRPIN_TH=36.42 PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_24_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_24_END_STABILITY=5.0700 PRIMER_RIGHT_24_END_STABILITY=3.5300 PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=8.9249 PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=6.8599 PRIMER_LEFT_25_PENALTY=2.999229 PRIMER_RIGHT_25_PENALTY=2.999229 PRIMER_INTERNAL_25_PENALTY=3.954555 PRIMER_LEFT_25_SEQUENCE=GATAGAAACGCCAACTCACTG PRIMER_RIGHT_25_SEQUENCE=CAGTGAGTTGGCGTTTCTATC PRIMER_INTERNAL_25_SEQUENCE=TCCAAAACGCAAAGCACGCTCCC PRIMER_LEFT_25=59,21 PRIMER_RIGHT_25=79,21 PRIMER_INTERNAL_25=120,23 PRIMER_LEFT_25_TM=57.001 PRIMER_RIGHT_25_TM=57.001 PRIMER_INTERNAL_25_TM=60.955 PRIMER_LEFT_25_GC_PERCENT=47.619 PRIMER_RIGHT_25_GC_PERCENT=47.619 PRIMER_INTERNAL_25_GC_PERCENT=56.522 PRIMER_INTERNAL_25_SELF_ANY_TH=0.00 PRIMER_LEFT_25_SELF_ANY_TH=0.00 PRIMER_RIGHT_25_SELF_ANY_TH=0.00 PRIMER_INTERNAL_25_SELF_END_TH=0.00 PRIMER_LEFT_25_SELF_END_TH=0.00 PRIMER_RIGHT_25_SELF_END_TH=0.00 PRIMER_LEFT_25_HAIRPIN_TH=0.00 PRIMER_RIGHT_25_HAIRPIN_TH=0.00 PRIMER_INTERNAL_25_HAIRPIN_TH=0.00 PRIMER_LEFT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_25_END_STABILITY=3.6600 PRIMER_RIGHT_25_END_STABILITY=2.0800 PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=19.5396 PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=18.4032 PRIMER_LEFT_26_PENALTY=2.999229 PRIMER_RIGHT_26_PENALTY=2.999229 PRIMER_INTERNAL_26_PENALTY=4.240075 PRIMER_LEFT_26_SEQUENCE=CTGATAGAAACGCCAACTCAC PRIMER_RIGHT_26_SEQUENCE=GTGAGTTGGCGTTTCTATCAG PRIMER_INTERNAL_26_SEQUENCE=CAAAACGCAAAGCACGCTCCCGA PRIMER_LEFT_26=57,21 PRIMER_RIGHT_26=77,21 PRIMER_INTERNAL_26=122,23 PRIMER_LEFT_26_TM=57.001 PRIMER_RIGHT_26_TM=57.001 PRIMER_INTERNAL_26_TM=61.240 PRIMER_LEFT_26_GC_PERCENT=47.619 PRIMER_RIGHT_26_GC_PERCENT=47.619 PRIMER_INTERNAL_26_GC_PERCENT=56.522 PRIMER_INTERNAL_26_SELF_ANY_TH=7.82 PRIMER_LEFT_26_SELF_ANY_TH=0.00 PRIMER_RIGHT_26_SELF_ANY_TH=0.00 PRIMER_INTERNAL_26_SELF_END_TH=0.00 PRIMER_LEFT_26_SELF_END_TH=0.00 PRIMER_RIGHT_26_SELF_END_TH=0.00 PRIMER_LEFT_26_HAIRPIN_TH=0.00 PRIMER_RIGHT_26_HAIRPIN_TH=0.00 PRIMER_INTERNAL_26_HAIRPIN_TH=36.42 PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_26_END_STABILITY=3.5100 PRIMER_RIGHT_26_END_STABILITY=2.9000 PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=13.7640 PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=15.9212 PRIMER_LEFT_27_PENALTY=3.455227 PRIMER_RIGHT_27_PENALTY=3.455227 PRIMER_INTERNAL_27_PENALTY=4.486709 PRIMER_LEFT_27_SEQUENCE=ATTCCAAAACGCAAAGCACGC PRIMER_RIGHT_27_SEQUENCE=GCGTGCTTTGCGTTTTGGAAT PRIMER_INTERNAL_27_SEQUENCE=TTCCAAAACGCAAAGCACGCTC PRIMER_LEFT_27=118,21 PRIMER_RIGHT_27=138,21 PRIMER_INTERNAL_27=119,22 PRIMER_LEFT_27_TM=61.455 PRIMER_RIGHT_27_TM=61.455 PRIMER_INTERNAL_27_TM=57.513 PRIMER_LEFT_27_GC_PERCENT=47.619 PRIMER_RIGHT_27_GC_PERCENT=47.619 PRIMER_INTERNAL_27_GC_PERCENT=50.000 PRIMER_INTERNAL_27_SELF_ANY_TH=0.00 PRIMER_LEFT_27_SELF_ANY_TH=0.00 PRIMER_RIGHT_27_SELF_ANY_TH=0.00 PRIMER_INTERNAL_27_SELF_END_TH=0.00 PRIMER_LEFT_27_SELF_END_TH=0.00 PRIMER_RIGHT_27_SELF_END_TH=0.00 PRIMER_LEFT_27_HAIRPIN_TH=0.00 PRIMER_RIGHT_27_HAIRPIN_TH=0.00 PRIMER_INTERNAL_27_HAIRPIN_TH=0.00 PRIMER_LEFT_27_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon PRIMER_LEFT_27_END_STABILITY=5.3400 PRIMER_RIGHT_27_END_STABILITY=3.0100 PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=1.8923 PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=11.3682 PRIMER_LEFT_28_PENALTY=3.677573 PRIMER_RIGHT_28_PENALTY=3.677573 PRIMER_INTERNAL_28_PENALTY=4.513970 PRIMER_LEFT_28_SEQUENCE=AAAACGCAAAGCACGCTCCC PRIMER_RIGHT_28_SEQUENCE=GGGAGCGTGCTTTGCGTTTT PRIMER_INTERNAL_28_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCC PRIMER_LEFT_28=123,20 PRIMER_RIGHT_28=142,20 PRIMER_INTERNAL_28=118,24 PRIMER_LEFT_28_TM=62.678 PRIMER_RIGHT_28_TM=62.678 PRIMER_INTERNAL_28_TM=59.486 PRIMER_LEFT_28_GC_PERCENT=55.000 PRIMER_RIGHT_28_GC_PERCENT=55.000 PRIMER_INTERNAL_28_GC_PERCENT=50.000 PRIMER_INTERNAL_28_SELF_ANY_TH=0.00 PRIMER_LEFT_28_SELF_ANY_TH=0.00 PRIMER_RIGHT_28_SELF_ANY_TH=0.00 PRIMER_INTERNAL_28_SELF_END_TH=0.00 PRIMER_LEFT_28_SELF_END_TH=0.00 PRIMER_RIGHT_28_SELF_END_TH=0.00 PRIMER_LEFT_28_HAIRPIN_TH=0.00 PRIMER_RIGHT_28_HAIRPIN_TH=37.44 PRIMER_INTERNAL_28_HAIRPIN_TH=0.00 PRIMER_LEFT_28_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1) PRIMER_RIGHT_28_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_28_END_STABILITY=4.3000 PRIMER_RIGHT_28_END_STABILITY=2.4300 PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=0.0612 PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=1.5624 PRIMER_LEFT_29_PENALTY=4.100862 PRIMER_RIGHT_29_PENALTY=4.100862 PRIMER_INTERNAL_29_PENALTY=5.029817 PRIMER_LEFT_29_SEQUENCE=GCAAAGCACGCTCCCGATC PRIMER_RIGHT_29_SEQUENCE=GATCGGGAGCGTGCTTTGC PRIMER_INTERNAL_29_SEQUENCE=AAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_29=128,19 PRIMER_RIGHT_29=146,19 PRIMER_INTERNAL_29=123,24 PRIMER_LEFT_29_TM=62.101 PRIMER_RIGHT_29_TM=62.101 PRIMER_INTERNAL_29_TM=61.030 PRIMER_LEFT_29_GC_PERCENT=63.158 PRIMER_RIGHT_29_GC_PERCENT=63.158 PRIMER_INTERNAL_29_GC_PERCENT=54.167 PRIMER_INTERNAL_29_SELF_ANY_TH=0.00 PRIMER_LEFT_29_SELF_ANY_TH=0.00 PRIMER_RIGHT_29_SELF_ANY_TH=0.00 PRIMER_INTERNAL_29_SELF_END_TH=0.00 PRIMER_LEFT_29_SELF_END_TH=0.00 PRIMER_RIGHT_29_SELF_END_TH=0.00 PRIMER_LEFT_29_HAIRPIN_TH=38.29 PRIMER_RIGHT_29_HAIRPIN_TH=0.00 PRIMER_INTERNAL_29_HAIRPIN_TH=36.42 PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon PRIMER_LEFT_29_END_STABILITY=3.6900 PRIMER_RIGHT_29_END_STABILITY=3.6800 PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=5.4030 PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_30_PENALTY=4.194272 PRIMER_RIGHT_30_PENALTY=4.194272 PRIMER_INTERNAL_30_PENALTY=5.052238 PRIMER_LEFT_30_SEQUENCE=AAACGCAAAGCACGCTCCC PRIMER_RIGHT_30_SEQUENCE=GGGAGCGTGCTTTGCGTTT PRIMER_INTERNAL_30_SEQUENCE=CCAAAACGCAAAGCACGCTCCCG PRIMER_LEFT_30=124,19 PRIMER_RIGHT_30=142,19 PRIMER_INTERNAL_30=121,23 PRIMER_LEFT_30_TM=62.194 PRIMER_RIGHT_30_TM=62.194 PRIMER_INTERNAL_30_TM=62.052 PRIMER_LEFT_30_GC_PERCENT=57.895 PRIMER_RIGHT_30_GC_PERCENT=57.895 PRIMER_INTERNAL_30_GC_PERCENT=60.870 PRIMER_INTERNAL_30_SELF_ANY_TH=1.50 PRIMER_LEFT_30_SELF_ANY_TH=0.00 PRIMER_RIGHT_30_SELF_ANY_TH=0.00 PRIMER_INTERNAL_30_SELF_END_TH=1.50 PRIMER_LEFT_30_SELF_END_TH=0.00 PRIMER_RIGHT_30_SELF_END_TH=0.00 PRIMER_LEFT_30_HAIRPIN_TH=0.00 PRIMER_RIGHT_30_HAIRPIN_TH=37.44 PRIMER_INTERNAL_30_HAIRPIN_TH=41.14 PRIMER_LEFT_30_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1) PRIMER_RIGHT_30_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_30_END_STABILITY=4.3000 PRIMER_RIGHT_30_END_STABILITY=3.6000 PRIMER_LEFT_30_TEMPLATE_MISPRIMING_TH=5.4315 PRIMER_RIGHT_30_TEMPLATE_MISPRIMING_TH=7.1743 PRIMER_LEFT_31_PENALTY=4.473143 PRIMER_RIGHT_31_PENALTY=4.473143 PRIMER_INTERNAL_31_PENALTY=5.152054 PRIMER_LEFT_31_SEQUENCE=TTCCAAAACGCAAAGCACGCT PRIMER_RIGHT_31_SEQUENCE=AGCGTGCTTTGCGTTTTGGAA PRIMER_INTERNAL_31_SEQUENCE=TTCCAAAACGCAAAGCACGCTCCC PRIMER_LEFT_31=119,21 PRIMER_RIGHT_31=139,21 PRIMER_INTERNAL_31=119,24 PRIMER_LEFT_31_TM=62.473 PRIMER_RIGHT_31_TM=62.473 PRIMER_INTERNAL_31_TM=61.152 PRIMER_LEFT_31_GC_PERCENT=47.619 PRIMER_RIGHT_31_GC_PERCENT=47.619 PRIMER_INTERNAL_31_GC_PERCENT=54.167 PRIMER_INTERNAL_31_SELF_ANY_TH=0.00 PRIMER_LEFT_31_SELF_ANY_TH=0.00 PRIMER_RIGHT_31_SELF_ANY_TH=0.00 PRIMER_INTERNAL_31_SELF_END_TH=0.00 PRIMER_LEFT_31_SELF_END_TH=0.00 PRIMER_RIGHT_31_SELF_END_TH=0.00 PRIMER_LEFT_31_HAIRPIN_TH=0.00 PRIMER_RIGHT_31_HAIRPIN_TH=37.44 PRIMER_INTERNAL_31_HAIRPIN_TH=0.00 PRIMER_LEFT_31_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_31_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_31_END_STABILITY=5.0700 PRIMER_RIGHT_31_END_STABILITY=3.5300 PRIMER_LEFT_31_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_31_TEMPLATE_MISPRIMING_TH=9.3017 PRIMER_LEFT_32_PENALTY=4.837196 PRIMER_RIGHT_32_PENALTY=4.837196 PRIMER_INTERNAL_32_PENALTY=5.256814 PRIMER_LEFT_32_SEQUENCE=TCCAAAACGCAAAGCACGCTC PRIMER_RIGHT_32_SEQUENCE=GAGCGTGCTTTGCGTTTTGGA PRIMER_INTERNAL_32_SEQUENCE=CAAAACGCAAAGCACGCTCCCGAT PRIMER_LEFT_32=120,21 PRIMER_RIGHT_32=140,21 PRIMER_INTERNAL_32=122,24 PRIMER_LEFT_32_TM=62.837 PRIMER_RIGHT_32_TM=62.837 PRIMER_INTERNAL_32_TM=61.257 PRIMER_LEFT_32_GC_PERCENT=52.381 PRIMER_RIGHT_32_GC_PERCENT=52.381 PRIMER_INTERNAL_32_GC_PERCENT=54.167 PRIMER_INTERNAL_32_SELF_ANY_TH=7.82 PRIMER_LEFT_32_SELF_ANY_TH=0.00 PRIMER_RIGHT_32_SELF_ANY_TH=0.00 PRIMER_INTERNAL_32_SELF_END_TH=0.00 PRIMER_LEFT_32_SELF_END_TH=0.00 PRIMER_RIGHT_32_SELF_END_TH=0.00 PRIMER_LEFT_32_HAIRPIN_TH=0.00 PRIMER_RIGHT_32_HAIRPIN_TH=37.44 PRIMER_INTERNAL_32_HAIRPIN_TH=36.42 PRIMER_LEFT_32_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_RIGHT_32_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_32_END_STABILITY=5.0300 PRIMER_RIGHT_32_END_STABILITY=3.5300 PRIMER_LEFT_32_TEMPLATE_MISPRIMING_TH=8.9959 PRIMER_RIGHT_32_TEMPLATE_MISPRIMING_TH=4.7683 PRIMER_INTERNAL_33_PENALTY=5.325970 PRIMER_INTERNAL_33_SEQUENCE=ATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_33=118,23 PRIMER_INTERNAL_33_TM=57.674 PRIMER_INTERNAL_33_GC_PERCENT=47.826 PRIMER_INTERNAL_33_SELF_ANY_TH=0.00 PRIMER_INTERNAL_33_SELF_END_TH=0.00 PRIMER_INTERNAL_33_HAIRPIN_TH=0.00 PRIMER_INTERNAL_34_PENALTY=5.931698 PRIMER_INTERNAL_34_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCC PRIMER_INTERNAL_34=117,25 PRIMER_INTERNAL_34_TM=59.068 PRIMER_INTERNAL_34_GC_PERCENT=48.000 PRIMER_INTERNAL_34_SELF_ANY_TH=0.00 PRIMER_INTERNAL_34_SELF_END_TH=0.00 PRIMER_INTERNAL_34_HAIRPIN_TH=0.00 PRIMER_INTERNAL_35_PENALTY=6.171494 PRIMER_INTERNAL_35_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCC PRIMER_INTERNAL_35=118,25 PRIMER_INTERNAL_35_TM=61.171 PRIMER_INTERNAL_35_GC_PERCENT=52.000 PRIMER_INTERNAL_35_SELF_ANY_TH=0.00 PRIMER_INTERNAL_35_SELF_END_TH=0.00 PRIMER_INTERNAL_35_HAIRPIN_TH=0.00 PRIMER_INTERNAL_36_PENALTY=6.354311 PRIMER_INTERNAL_36_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCC PRIMER_INTERNAL_36=116,26 PRIMER_INTERNAL_36_TM=59.646 PRIMER_INTERNAL_36_GC_PERCENT=50.000 PRIMER_INTERNAL_36_SELF_ANY_TH=0.00 PRIMER_INTERNAL_36_SELF_END_TH=0.00 PRIMER_INTERNAL_36_HAIRPIN_TH=0.00 PRIMER_INTERNAL_37_PENALTY=6.689016 PRIMER_INTERNAL_37_SEQUENCE=TATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_37=117,24 PRIMER_INTERNAL_37_TM=57.311 PRIMER_INTERNAL_37_GC_PERCENT=45.833 PRIMER_INTERNAL_37_SELF_ANY_TH=0.00 PRIMER_INTERNAL_37_SELF_END_TH=0.00 PRIMER_INTERNAL_37_HAIRPIN_TH=0.00 PRIMER_INTERNAL_38_PENALTY=6.689016 PRIMER_INTERNAL_38_SEQUENCE=TCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_38=115,24 PRIMER_INTERNAL_38_TM=57.311 PRIMER_INTERNAL_38_GC_PERCENT=45.833 PRIMER_INTERNAL_38_SELF_ANY_TH=0.00 PRIMER_INTERNAL_38_SELF_END_TH=0.00 PRIMER_INTERNAL_38_HAIRPIN_TH=0.00 PRIMER_INTERNAL_39_PENALTY=6.694952 PRIMER_INTERNAL_39_SEQUENCE=CTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_39=116,24 PRIMER_INTERNAL_39_TM=57.305 PRIMER_INTERNAL_39_GC_PERCENT=45.833 PRIMER_INTERNAL_39_SELF_ANY_TH=0.00 PRIMER_INTERNAL_39_SELF_END_TH=0.00 PRIMER_INTERNAL_39_HAIRPIN_TH=0.00 PRIMER_INTERNAL_40_PENALTY=6.707310 PRIMER_INTERNAL_40_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCC PRIMER_INTERNAL_40=117,26 PRIMER_INTERNAL_40_TM=60.707 PRIMER_INTERNAL_40_GC_PERCENT=50.000 PRIMER_INTERNAL_40_SELF_ANY_TH=0.00 PRIMER_INTERNAL_40_SELF_END_TH=0.00 PRIMER_INTERNAL_40_HAIRPIN_TH=0.00 PRIMER_INTERNAL_41_PENALTY=6.724062 PRIMER_INTERNAL_41_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_41=115,25 PRIMER_INTERNAL_41_TM=58.276 PRIMER_INTERNAL_41_GC_PERCENT=44.000 PRIMER_INTERNAL_41_SELF_ANY_TH=0.00 PRIMER_INTERNAL_41_SELF_END_TH=0.00 PRIMER_INTERNAL_41_HAIRPIN_TH=0.00 PRIMER_INTERNAL_42_PENALTY=6.744217 PRIMER_INTERNAL_42_SEQUENCE=CAAAACGCAAAGCACGCTCCCGATC PRIMER_INTERNAL_42=122,25 PRIMER_INTERNAL_42_TM=61.744 PRIMER_INTERNAL_42_GC_PERCENT=56.000 PRIMER_INTERNAL_42_SELF_ANY_TH=0.00 PRIMER_INTERNAL_42_SELF_END_TH=0.00 PRIMER_INTERNAL_42_HAIRPIN_TH=36.42 PRIMER_INTERNAL_43_PENALTY=6.785590 PRIMER_INTERNAL_43_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_43=114,25 PRIMER_INTERNAL_43_TM=58.214 PRIMER_INTERNAL_43_GC_PERCENT=48.000 PRIMER_INTERNAL_43_SELF_ANY_TH=0.00 PRIMER_INTERNAL_43_SELF_END_TH=0.00 PRIMER_INTERNAL_43_HAIRPIN_TH=0.00 PRIMER_INTERNAL_44_PENALTY=6.889273 PRIMER_INTERNAL_44_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_44=114,26 PRIMER_INTERNAL_44_TM=59.111 PRIMER_INTERNAL_44_GC_PERCENT=46.154 PRIMER_INTERNAL_44_SELF_ANY_TH=0.00 PRIMER_INTERNAL_44_SELF_END_TH=0.00 PRIMER_INTERNAL_44_HAIRPIN_TH=0.00 PRIMER_INTERNAL_45_PENALTY=6.889273 PRIMER_INTERNAL_45_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_45=113,26 PRIMER_INTERNAL_45_TM=59.111 PRIMER_INTERNAL_45_GC_PERCENT=46.154 PRIMER_INTERNAL_45_SELF_ANY_TH=0.00 PRIMER_INTERNAL_45_SELF_END_TH=0.00 PRIMER_INTERNAL_45_HAIRPIN_TH=0.00 PRIMER_INTERNAL_46_PENALTY=6.910113 PRIMER_INTERNAL_46_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGA PRIMER_INTERNAL_46=121,24 PRIMER_INTERNAL_46_TM=62.910 PRIMER_INTERNAL_46_GC_PERCENT=58.333 PRIMER_INTERNAL_46_SELF_ANY_TH=7.82 PRIMER_INTERNAL_46_SELF_END_TH=0.00 PRIMER_INTERNAL_46_HAIRPIN_TH=36.42 PRIMER_INTERNAL_47_PENALTY=6.910113 PRIMER_INTERNAL_47_SEQUENCE=TCCAAAACGCAAAGCACGCTCCCG PRIMER_INTERNAL_47=120,24 PRIMER_INTERNAL_47_TM=62.910 PRIMER_INTERNAL_47_GC_PERCENT=58.333 PRIMER_INTERNAL_47_SELF_ANY_TH=1.50 PRIMER_INTERNAL_47_SELF_END_TH=1.50 PRIMER_INTERNAL_47_HAIRPIN_TH=41.14 PRIMER_INTERNAL_48_PENALTY=7.008353 PRIMER_INTERNAL_48_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_48=116,25 PRIMER_INTERNAL_48_TM=57.992 PRIMER_INTERNAL_48_GC_PERCENT=48.000 PRIMER_INTERNAL_48_SELF_ANY_TH=0.00 PRIMER_INTERNAL_48_SELF_END_TH=0.00 PRIMER_INTERNAL_48_HAIRPIN_TH=0.00 PRIMER_INTERNAL_49_PENALTY=7.035358 PRIMER_INTERNAL_49_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_49=113,27 PRIMER_INTERNAL_49_TM=59.965 PRIMER_INTERNAL_49_GC_PERCENT=44.444 PRIMER_INTERNAL_49_SELF_ANY_TH=0.00 PRIMER_INTERNAL_49_SELF_END_TH=0.00 PRIMER_INTERNAL_49_HAIRPIN_TH=0.00 PRIMER_INTERNAL_50_PENALTY=7.102160 PRIMER_INTERNAL_50_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_50=115,26 PRIMER_INTERNAL_50_TM=58.898 PRIMER_INTERNAL_50_GC_PERCENT=46.154 PRIMER_INTERNAL_50_SELF_ANY_TH=0.00 PRIMER_INTERNAL_50_SELF_END_TH=0.00 PRIMER_INTERNAL_50_HAIRPIN_TH=0.00 PRIMER_INTERNAL_51_PENALTY=7.334450 PRIMER_INTERNAL_51_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_51=114,27 PRIMER_INTERNAL_51_TM=59.666 PRIMER_INTERNAL_51_GC_PERCENT=48.148 PRIMER_INTERNAL_51_SELF_ANY_TH=0.00 PRIMER_INTERNAL_51_SELF_END_TH=0.00 PRIMER_INTERNAL_51_HAIRPIN_TH=0.00 PRIMER_INTERNAL_52_PENALTY=7.480679 PRIMER_INTERNAL_52_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTCC PRIMER_INTERNAL_52=115,27 PRIMER_INTERNAL_52_TM=60.481 PRIMER_INTERNAL_52_GC_PERCENT=48.148 PRIMER_INTERNAL_52_SELF_ANY_TH=0.00 PRIMER_INTERNAL_52_SELF_END_TH=0.00 PRIMER_INTERNAL_52_HAIRPIN_TH=0.00 PRIMER_INTERNAL_53_PENALTY=7.647244 PRIMER_INTERNAL_53_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_53=112,27 PRIMER_INTERNAL_53_TM=59.353 PRIMER_INTERNAL_53_GC_PERCENT=44.444 PRIMER_INTERNAL_53_SELF_ANY_TH=0.00 PRIMER_INTERNAL_53_SELF_END_TH=0.00 PRIMER_INTERNAL_53_HAIRPIN_TH=0.00 PRIMER_INTERNAL_54_PENALTY=7.744113 PRIMER_INTERNAL_54_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGT PRIMER_INTERNAL_54=55,26 PRIMER_INTERNAL_54_TM=58.256 PRIMER_INTERNAL_54_GC_PERCENT=46.154 PRIMER_INTERNAL_54_SELF_ANY_TH=0.00 PRIMER_INTERNAL_54_SELF_END_TH=0.00 PRIMER_INTERNAL_54_HAIRPIN_TH=0.00 PRIMER_INTERNAL_55_PENALTY=7.867066 PRIMER_INTERNAL_55_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGAT PRIMER_INTERNAL_55=121,25 PRIMER_INTERNAL_55_TM=62.867 PRIMER_INTERNAL_55_GC_PERCENT=56.000 PRIMER_INTERNAL_55_SELF_ANY_TH=7.82 PRIMER_INTERNAL_55_SELF_END_TH=0.00 PRIMER_INTERNAL_55_HAIRPIN_TH=36.42 PRIMER_INTERNAL_56_PENALTY=7.916609 PRIMER_INTERNAL_56_SEQUENCE=TGACCTGATAGAAACGCCAACTCAC PRIMER_INTERNAL_56=53,25 PRIMER_INTERNAL_56_TM=57.083 PRIMER_INTERNAL_56_GC_PERCENT=48.000 PRIMER_INTERNAL_56_SELF_ANY_TH=0.00 PRIMER_INTERNAL_56_SELF_END_TH=0.00 PRIMER_INTERNAL_56_HAIRPIN_TH=0.00 PRIMER_INTERNAL_57_PENALTY=7.922893 PRIMER_INTERNAL_57_SEQUENCE=CCTGATAGAAACGCCAACTCACTGT PRIMER_INTERNAL_57=56,25 PRIMER_INTERNAL_57_TM=57.077 PRIMER_INTERNAL_57_GC_PERCENT=48.000 PRIMER_INTERNAL_57_SELF_ANY_TH=0.00 PRIMER_INTERNAL_57_SELF_END_TH=0.00 PRIMER_INTERNAL_57_HAIRPIN_TH=0.00 PRIMER_INTERNAL_58_PENALTY=7.922893 PRIMER_INTERNAL_58_SEQUENCE=ACCTGATAGAAACGCCAACTCACTG PRIMER_INTERNAL_58=55,25 PRIMER_INTERNAL_58_TM=57.077 PRIMER_INTERNAL_58_GC_PERCENT=48.000 PRIMER_INTERNAL_58_SELF_ANY_TH=0.00 PRIMER_INTERNAL_58_SELF_END_TH=0.00 PRIMER_INTERNAL_58_HAIRPIN_TH=0.00 PRIMER_INTERNAL_59_PENALTY=7.963894 PRIMER_INTERNAL_59_SEQUENCE=TGACCTGATAGAAACGCCAACTCACT PRIMER_INTERNAL_59=53,26 PRIMER_INTERNAL_59_TM=58.036 PRIMER_INTERNAL_59_GC_PERCENT=46.154 PRIMER_INTERNAL_59_SELF_ANY_TH=0.00 PRIMER_INTERNAL_59_SELF_END_TH=0.00 PRIMER_INTERNAL_59_HAIRPIN_TH=0.00 PRIMER_INTERNAL_60_PENALTY=8.131843 PRIMER_INTERNAL_60_SEQUENCE=TGACCTGATAGAAACGCCAACTCACTG PRIMER_INTERNAL_60=53,27 PRIMER_INTERNAL_60_TM=58.868 PRIMER_INTERNAL_60_GC_PERCENT=48.148 PRIMER_INTERNAL_60_SELF_ANY_TH=0.00 PRIMER_INTERNAL_60_SELF_END_TH=0.00 PRIMER_INTERNAL_60_HAIRPIN_TH=0.00 PRIMER_INTERNAL_61_PENALTY=8.132460 PRIMER_INTERNAL_61_SEQUENCE=GACCTGATAGAAACGCCAACTCACTGT PRIMER_INTERNAL_61=54,27 PRIMER_INTERNAL_61_TM=58.868 PRIMER_INTERNAL_61_GC_PERCENT=48.148 PRIMER_INTERNAL_61_SELF_ANY_TH=0.00 PRIMER_INTERNAL_61_SELF_END_TH=0.00 PRIMER_INTERNAL_61_HAIRPIN_TH=0.00 PRIMER_INTERNAL_62_PENALTY=8.193320 PRIMER_INTERNAL_62_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCCC PRIMER_INTERNAL_62=116,27 PRIMER_INTERNAL_62_TM=61.193 PRIMER_INTERNAL_62_GC_PERCENT=51.852 PRIMER_INTERNAL_62_SELF_ANY_TH=0.00 PRIMER_INTERNAL_62_SELF_END_TH=0.00 PRIMER_INTERNAL_62_HAIRPIN_TH=0.00 PRIMER_INTERNAL_63_PENALTY=8.237701 PRIMER_INTERNAL_63_SEQUENCE=GACCTGATAGAAACGCCAACTCACTG PRIMER_INTERNAL_63=54,26 PRIMER_INTERNAL_63_TM=57.762 PRIMER_INTERNAL_63_GC_PERCENT=50.000 PRIMER_INTERNAL_63_SELF_ANY_TH=0.00 PRIMER_INTERNAL_63_SELF_END_TH=0.00 PRIMER_INTERNAL_63_HAIRPIN_TH=0.00 PRIMER_INTERNAL_64_PENALTY=8.755267 PRIMER_INTERNAL_64_SEQUENCE=ATGACCTGATAGAAACGCCAACTCAC PRIMER_INTERNAL_64=52,26 PRIMER_INTERNAL_64_TM=57.245 PRIMER_INTERNAL_64_GC_PERCENT=46.154 PRIMER_INTERNAL_64_SELF_ANY_TH=0.00 PRIMER_INTERNAL_64_SELF_END_TH=0.00 PRIMER_INTERNAL_64_HAIRPIN_TH=0.00 PRIMER_INTERNAL_65_PENALTY=8.758760 PRIMER_INTERNAL_65_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACG PRIMER_INTERNAL_65=112,26 PRIMER_INTERNAL_65_TM=57.241 PRIMER_INTERNAL_65_GC_PERCENT=42.308 PRIMER_INTERNAL_65_SELF_ANY_TH=0.00 PRIMER_INTERNAL_65_SELF_END_TH=0.00 PRIMER_INTERNAL_65_HAIRPIN_TH=0.00 PRIMER_INTERNAL_66_PENALTY=8.838967 PRIMER_INTERNAL_66_SEQUENCE=ATGACCTGATAGAAACGCCAACTCACT PRIMER_INTERNAL_66=52,27 PRIMER_INTERNAL_66_TM=58.161 PRIMER_INTERNAL_66_GC_PERCENT=44.444 PRIMER_INTERNAL_66_SELF_ANY_TH=0.00 PRIMER_INTERNAL_66_SELF_END_TH=0.00 PRIMER_INTERNAL_66_HAIRPIN_TH=0.00 PRIMER_INTERNAL_67_PENALTY=8.978186 PRIMER_INTERNAL_67_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCCG PRIMER_INTERNAL_67=118,26 PRIMER_INTERNAL_67_TM=62.978 PRIMER_INTERNAL_67_GC_PERCENT=53.846 PRIMER_INTERNAL_67_SELF_ANY_TH=1.50 PRIMER_INTERNAL_67_SELF_END_TH=1.50 PRIMER_INTERNAL_67_HAIRPIN_TH=41.14 PRIMER_INTERNAL_68_PENALTY=9.099564 PRIMER_INTERNAL_68_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGTA PRIMER_INTERNAL_68=55,27 PRIMER_INTERNAL_68_TM=57.900 PRIMER_INTERNAL_68_GC_PERCENT=44.444 PRIMER_INTERNAL_68_SELF_ANY_TH=0.00 PRIMER_INTERNAL_68_SELF_END_TH=0.00 PRIMER_INTERNAL_68_HAIRPIN_TH=0.00 PRIMER_INTERNAL_69_PENALTY=9.448747 PRIMER_INTERNAL_69_SEQUENCE=AAAGTCTATTCCAAAACGCAAAGCACG PRIMER_INTERNAL_69=111,27 PRIMER_INTERNAL_69_TM=57.551 PRIMER_INTERNAL_69_GC_PERCENT=40.741 PRIMER_INTERNAL_69_SELF_ANY_TH=0.00 PRIMER_INTERNAL_69_SELF_END_TH=0.00 PRIMER_INTERNAL_69_HAIRPIN_TH=0.00 PRIMER_INTERNAL_70_PENALTY=9.472089 PRIMER_INTERNAL_70_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCCG PRIMER_INTERNAL_70=117,27 PRIMER_INTERNAL_70_TM=62.472 PRIMER_INTERNAL_70_GC_PERCENT=51.852 PRIMER_INTERNAL_70_SELF_ANY_TH=1.50 PRIMER_INTERNAL_70_SELF_END_TH=1.50 PRIMER_INTERNAL_70_HAIRPIN_TH=41.14 PRIMER_INTERNAL_71_PENALTY=9.912698 PRIMER_INTERNAL_71_SEQUENCE=CGTTGTCAAAGTCTATTCCAAAACGCA PRIMER_INTERNAL_71=104,27 PRIMER_INTERNAL_71_TM=57.087 PRIMER_INTERNAL_71_GC_PERCENT=40.741 PRIMER_INTERNAL_71_SELF_ANY_TH=0.00 PRIMER_INTERNAL_71_SELF_END_TH=0.00 PRIMER_INTERNAL_71_HAIRPIN_TH=0.00 PRIMER_INTERNAL_72_PENALTY=9.914131 PRIMER_INTERNAL_72_SEQUENCE=ACGTTGTCAAAGTCTATTCCAAAACGC PRIMER_INTERNAL_72=103,27 PRIMER_INTERNAL_72_TM=57.086 PRIMER_INTERNAL_72_GC_PERCENT=40.741 PRIMER_INTERNAL_72_SELF_ANY_TH=0.00 PRIMER_INTERNAL_72_SELF_END_TH=0.00 PRIMER_INTERNAL_72_HAIRPIN_TH=0.00 = PRIMER_TASK=pick_primer_list PRIMER_PRODUCT_SIZE_RANGE=75-200 P3_FILE_FLAG=1 SEQUENCE_ID=pick_primer_list_and_file_flag_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_LEFT_EXPLAIN=considered 175, too many Ns 19, overlap target 25, low tm 64, high tm 12, high repeat similarity 3, ok 52 PRIMER_RIGHT_EXPLAIN=considered 174, too many Ns 21, overlap target 23, low tm 64, high tm 12, high hairpin stability 16, high repeat similarity 3, ok 35 PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, ok 197 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=52 PRIMER_RIGHT_NUM_RETURNED=35 PRIMER_INTERNAL_NUM_RETURNED=197 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.527529 PRIMER_RIGHT_0_PENALTY=0.527529 PRIMER_INTERNAL_0_PENALTY=0.727038 PRIMER_LEFT_0_SEQUENCE=TTAGTGGGCCATTCGATCCC PRIMER_RIGHT_0_SEQUENCE=GGGATCGAATGGCCCACTAA PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC PRIMER_LEFT_0=61,20 PRIMER_RIGHT_0=80,20 PRIMER_INTERNAL_0=66,20 PRIMER_LEFT_0_TM=59.528 PRIMER_RIGHT_0_TM=59.528 PRIMER_INTERNAL_0_TM=59.273 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=70.000 PRIMER_INTERNAL_0_SELF_ANY_TH=2.18 PRIMER_LEFT_0_SELF_ANY_TH=5.82 PRIMER_RIGHT_0_SELF_ANY_TH=10.16 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=5.82 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=32.36 PRIMER_INTERNAL_0_HAIRPIN_TH=41.60 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=2.2400 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=2.6459 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0266 PRIMER_LEFT_1_PENALTY=0.606923 PRIMER_RIGHT_1_PENALTY=0.606923 PRIMER_INTERNAL_1_PENALTY=1.416589 PRIMER_LEFT_1_SEQUENCE=CCCGACCCAAATCGATAGCT PRIMER_RIGHT_1_SEQUENCE=AGCTATCGATTTGGGTCGGG PRIMER_INTERNAL_1_SEQUENCE=GTGGGCCATTCGATCCCGACC PRIMER_LEFT_1=78,20 PRIMER_RIGHT_1=97,20 PRIMER_INTERNAL_1=64,21 PRIMER_LEFT_1_TM=59.607 PRIMER_RIGHT_1_TM=59.607 PRIMER_INTERNAL_1_TM=59.583 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=66.667 PRIMER_INTERNAL_1_SELF_ANY_TH=2.53 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=45.13 PRIMER_RIGHT_1_HAIRPIN_TH=41.74 PRIMER_INTERNAL_1_HAIRPIN_TH=41.60 PRIMER_LEFT_1_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence PRIMER_RIGHT_1_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_LEFT_1_END_STABILITY=3.3200 PRIMER_RIGHT_1_END_STABILITY=5.1400 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=0.0186 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=7.7569 PRIMER_LEFT_2_PENALTY=0.608403 PRIMER_RIGHT_2_PENALTY=0.608403 PRIMER_INTERNAL_2_PENALTY=1.438741 PRIMER_LEFT_2_SEQUENCE=TCCCGACCCAAATCGATAGC PRIMER_RIGHT_2_SEQUENCE=GCTATCGATTTGGGTCGGGA PRIMER_INTERNAL_2_SEQUENCE=GGCCATTCGATCCCGACCCA PRIMER_LEFT_2=77,20 PRIMER_RIGHT_2=96,20 PRIMER_INTERNAL_2=67,20 PRIMER_LEFT_2_TM=59.608 PRIMER_RIGHT_2_TM=59.608 PRIMER_INTERNAL_2_TM=58.561 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=65.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=1.64 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=45.13 PRIMER_RIGHT_2_HAIRPIN_TH=41.74 PRIMER_INTERNAL_2_HAIRPIN_TH=41.60 PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_2_END_STABILITY=2.9700 PRIMER_RIGHT_2_END_STABILITY=5.1400 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=5.2033 PRIMER_LEFT_3_PENALTY=0.713597 PRIMER_RIGHT_3_PENALTY=0.713597 PRIMER_INTERNAL_3_PENALTY=1.438741 PRIMER_LEFT_3_SEQUENCE=GACATGACACTGACGATGCA PRIMER_RIGHT_3_SEQUENCE=TGCATCGTCAGTGTCATGTC PRIMER_INTERNAL_3_SEQUENCE=TGGGCCATTCGATCCCGACC PRIMER_LEFT_3=11,20 PRIMER_RIGHT_3=30,20 PRIMER_INTERNAL_3=65,20 PRIMER_LEFT_3_TM=58.286 PRIMER_RIGHT_3_TM=58.286 PRIMER_INTERNAL_3_TM=58.561 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_SELF_ANY_TH=4.28 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=41.60 PRIMER_LEFT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_3_END_STABILITY=3.9600 PRIMER_RIGHT_3_END_STABILITY=3.0600 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=4.9455 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.9996 PRIMER_LEFT_4_PENALTY=0.966496 PRIMER_RIGHT_4_PENALTY=0.966496 PRIMER_INTERNAL_4_PENALTY=1.650306 PRIMER_LEFT_4_SEQUENCE=CGATCCCGACCCAAATCGAT PRIMER_RIGHT_4_SEQUENCE=ATCGATTTGGGTCGGGATCG PRIMER_INTERNAL_4_SEQUENCE=GGGCCATTCGATCCCGACCCA PRIMER_LEFT_4=74,20 PRIMER_RIGHT_4=93,20 PRIMER_INTERNAL_4=66,21 PRIMER_LEFT_4_TM=59.966 PRIMER_RIGHT_4_TM=59.966 PRIMER_INTERNAL_4_TM=60.650 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=66.667 PRIMER_INTERNAL_4_SELF_ANY_TH=2.16 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=45.13 PRIMER_RIGHT_4_HAIRPIN_TH=41.74 PRIMER_INTERNAL_4_HAIRPIN_TH=41.60 PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_4_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_4_END_STABILITY=3.5900 PRIMER_RIGHT_4_END_STABILITY=3.6900 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=2.7399 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=6.2638 PRIMER_LEFT_5_PENALTY=0.967319 PRIMER_RIGHT_5_PENALTY=1.001812 PRIMER_INTERNAL_5_PENALTY=1.650306 PRIMER_LEFT_5_SEQUENCE=CATTCGATCCCGACCCAAAT PRIMER_RIGHT_5_SEQUENCE=GTCTGCATCGTCAGTGTCAT PRIMER_INTERNAL_5_SEQUENCE=TGGGCCATTCGATCCCGACCC PRIMER_LEFT_5=70,20 PRIMER_RIGHT_5=33,20 PRIMER_INTERNAL_5=65,21 PRIMER_LEFT_5_TM=58.033 PRIMER_RIGHT_5_TM=57.998 PRIMER_INTERNAL_5_TM=60.650 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=50.000 PRIMER_INTERNAL_5_GC_PERCENT=66.667 PRIMER_INTERNAL_5_SELF_ANY_TH=4.28 PRIMER_LEFT_5_SELF_ANY_TH=0.00 PRIMER_RIGHT_5_SELF_ANY_TH=0.00 PRIMER_INTERNAL_5_SELF_END_TH=0.51 PRIMER_LEFT_5_SELF_END_TH=0.00 PRIMER_RIGHT_5_SELF_END_TH=0.00 PRIMER_LEFT_5_HAIRPIN_TH=45.50 PRIMER_RIGHT_5_HAIRPIN_TH=0.00 PRIMER_INTERNAL_5_HAIRPIN_TH=41.60 PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_5_END_STABILITY=2.3200 PRIMER_RIGHT_5_END_STABILITY=3.0600 PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=2.1397 PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_6_PENALTY=1.000429 PRIMER_RIGHT_6_PENALTY=1.001812 PRIMER_INTERNAL_6_PENALTY=2.095978 PRIMER_LEFT_6_SEQUENCE=CATGACACTGACGATGCAGA PRIMER_RIGHT_6_SEQUENCE=GCATCGTCAGTGTCATGTCT PRIMER_INTERNAL_6_SEQUENCE=GGCCATTCGATCCCGACCCAA PRIMER_LEFT_6=13,20 PRIMER_RIGHT_6=29,20 PRIMER_INTERNAL_6=67,21 PRIMER_LEFT_6_TM=58.000 PRIMER_RIGHT_6_TM=57.998 PRIMER_INTERNAL_6_TM=58.904 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_INTERNAL_6_GC_PERCENT=61.905 PRIMER_INTERNAL_6_SELF_ANY_TH=0.00 PRIMER_LEFT_6_SELF_ANY_TH=10.65 PRIMER_RIGHT_6_SELF_ANY_TH=0.00 PRIMER_INTERNAL_6_SELF_END_TH=0.00 PRIMER_LEFT_6_SELF_END_TH=0.00 PRIMER_RIGHT_6_SELF_END_TH=0.00 PRIMER_LEFT_6_HAIRPIN_TH=0.00 PRIMER_RIGHT_6_HAIRPIN_TH=0.00 PRIMER_INTERNAL_6_HAIRPIN_TH=41.60 PRIMER_LEFT_6_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_6_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_6_END_STABILITY=4.2600 PRIMER_RIGHT_6_END_STABILITY=3.4100 PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=1.1151 PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=15.2988 PRIMER_LEFT_7_PENALTY=1.001812 PRIMER_RIGHT_7_PENALTY=1.142220 PRIMER_INTERNAL_7_PENALTY=2.360266 PRIMER_LEFT_7_SEQUENCE=ATGACACTGACGATGCAGAC PRIMER_RIGHT_7_SEQUENCE=GGGATCGAATGGCCCACTA PRIMER_INTERNAL_7_SEQUENCE=AGTGGGCCATTCGATCCCGAC PRIMER_LEFT_7=14,20 PRIMER_RIGHT_7=80,19 PRIMER_INTERNAL_7=63,21 PRIMER_LEFT_7_TM=57.998 PRIMER_RIGHT_7_TM=58.858 PRIMER_INTERNAL_7_TM=58.640 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=57.895 PRIMER_INTERNAL_7_GC_PERCENT=61.905 PRIMER_INTERNAL_7_SELF_ANY_TH=3.57 PRIMER_LEFT_7_SELF_ANY_TH=4.19 PRIMER_RIGHT_7_SELF_ANY_TH=10.16 PRIMER_INTERNAL_7_SELF_END_TH=3.02 PRIMER_LEFT_7_SELF_END_TH=0.00 PRIMER_RIGHT_7_SELF_END_TH=0.00 PRIMER_LEFT_7_HAIRPIN_TH=0.00 PRIMER_RIGHT_7_HAIRPIN_TH=32.36 PRIMER_INTERNAL_7_HAIRPIN_TH=41.60 PRIMER_LEFT_7_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_7_END_STABILITY=3.5100 PRIMER_RIGHT_7_END_STABILITY=2.7400 PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=8.4593 PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=7.9828 PRIMER_LEFT_8_PENALTY=1.001812 PRIMER_RIGHT_8_PENALTY=1.316792 PRIMER_INTERNAL_8_PENALTY=2.456420 PRIMER_LEFT_8_SEQUENCE=AGACATGACACTGACGATGC PRIMER_RIGHT_8_SEQUENCE=CGTAGCTATCGATTTGGGTCG PRIMER_INTERNAL_8_SEQUENCE=AGTGGGCCATTCGATCCCGA PRIMER_LEFT_8=10,20 PRIMER_RIGHT_8=100,21 PRIMER_INTERNAL_8=63,20 PRIMER_LEFT_8_TM=57.998 PRIMER_RIGHT_8_TM=58.683 PRIMER_INTERNAL_8_TM=57.544 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=52.381 PRIMER_INTERNAL_8_GC_PERCENT=60.000 PRIMER_INTERNAL_8_SELF_ANY_TH=3.28 PRIMER_LEFT_8_SELF_ANY_TH=0.00 PRIMER_RIGHT_8_SELF_ANY_TH=9.28 PRIMER_INTERNAL_8_SELF_END_TH=0.00 PRIMER_LEFT_8_SELF_END_TH=0.00 PRIMER_RIGHT_8_SELF_END_TH=4.23 PRIMER_LEFT_8_HAIRPIN_TH=0.00 PRIMER_RIGHT_8_HAIRPIN_TH=42.74 PRIMER_INTERNAL_8_HAIRPIN_TH=41.96 PRIMER_LEFT_8_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, MER4A a consensus PRIMER_LEFT_8_END_STABILITY=3.9100 PRIMER_RIGHT_8_END_STABILITY=4.7900 PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=8.8342 PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=11.4930 PRIMER_LEFT_9_PENALTY=1.107410 PRIMER_RIGHT_9_PENALTY=1.383135 PRIMER_INTERNAL_9_PENALTY=2.511372 PRIMER_LEFT_9_SEQUENCE=CCATTCGATCCCGACCCAAA PRIMER_RIGHT_9_SEQUENCE=TAGCTATCGATTTGGGTCGGG PRIMER_INTERNAL_9_SEQUENCE=GTGGGCCATTCGATCCCGAC PRIMER_LEFT_9=69,20 PRIMER_RIGHT_9=98,21 PRIMER_INTERNAL_9=64,20 PRIMER_LEFT_9_TM=60.107 PRIMER_RIGHT_9_TM=59.383 PRIMER_INTERNAL_9_TM=57.489 PRIMER_LEFT_9_GC_PERCENT=55.000 PRIMER_RIGHT_9_GC_PERCENT=52.381 PRIMER_INTERNAL_9_GC_PERCENT=65.000 PRIMER_INTERNAL_9_SELF_ANY_TH=0.97 PRIMER_LEFT_9_SELF_ANY_TH=0.00 PRIMER_RIGHT_9_SELF_ANY_TH=0.00 PRIMER_INTERNAL_9_SELF_END_TH=0.00 PRIMER_LEFT_9_SELF_END_TH=0.00 PRIMER_RIGHT_9_SELF_END_TH=0.00 PRIMER_LEFT_9_HAIRPIN_TH=45.50 PRIMER_RIGHT_9_HAIRPIN_TH=41.74 PRIMER_INTERNAL_9_HAIRPIN_TH=41.60 PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_LEFT_9_END_STABILITY=3.2800 PRIMER_RIGHT_9_END_STABILITY=5.1400 PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=7.7569 PRIMER_LEFT_10_PENALTY=1.126409 PRIMER_RIGHT_10_PENALTY=1.559940 PRIMER_INTERNAL_10_PENALTY=2.626619 PRIMER_LEFT_10_SEQUENCE=CATTCGATCCCGACCCAAATC PRIMER_RIGHT_10_SEQUENCE=TCGATTTGGGTCGGGATCG PRIMER_INTERNAL_10_SEQUENCE=AGTGGGCCATTCGATCCCGACC PRIMER_LEFT_10=70,21 PRIMER_RIGHT_10=92,19 PRIMER_INTERNAL_10=63,22 PRIMER_LEFT_10_TM=59.126 PRIMER_RIGHT_10_TM=59.560 PRIMER_INTERNAL_10_TM=60.627 PRIMER_LEFT_10_GC_PERCENT=52.381 PRIMER_RIGHT_10_GC_PERCENT=57.895 PRIMER_INTERNAL_10_GC_PERCENT=63.636 PRIMER_INTERNAL_10_SELF_ANY_TH=5.61 PRIMER_LEFT_10_SELF_ANY_TH=0.00 PRIMER_RIGHT_10_SELF_ANY_TH=0.00 PRIMER_INTERNAL_10_SELF_END_TH=0.00 PRIMER_LEFT_10_SELF_END_TH=0.00 PRIMER_RIGHT_10_SELF_END_TH=0.00 PRIMER_LEFT_10_HAIRPIN_TH=45.50 PRIMER_RIGHT_10_HAIRPIN_TH=41.74 PRIMER_INTERNAL_10_HAIRPIN_TH=41.60 PRIMER_LEFT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_RIGHT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_10_END_STABILITY=2.1700 PRIMER_RIGHT_10_END_STABILITY=3.6900 PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=3.6627 PRIMER_LEFT_11_PENALTY=1.142220 PRIMER_RIGHT_11_PENALTY=1.559940 PRIMER_INTERNAL_11_PENALTY=2.783921 PRIMER_LEFT_11_SEQUENCE=TAGTGGGCCATTCGATCCC PRIMER_RIGHT_11_SEQUENCE=CGATTTGGGTCGGGATCGA PRIMER_INTERNAL_11_SEQUENCE=GGCCATTCGATCCCGACCCAAA PRIMER_LEFT_11=62,19 PRIMER_RIGHT_11=91,19 PRIMER_INTERNAL_11=67,22 PRIMER_LEFT_11_TM=58.858 PRIMER_RIGHT_11_TM=59.560 PRIMER_INTERNAL_11_TM=59.216 PRIMER_LEFT_11_GC_PERCENT=57.895 PRIMER_RIGHT_11_GC_PERCENT=57.895 PRIMER_INTERNAL_11_GC_PERCENT=59.091 PRIMER_INTERNAL_11_SELF_ANY_TH=0.00 PRIMER_LEFT_11_SELF_ANY_TH=5.82 PRIMER_RIGHT_11_SELF_ANY_TH=0.00 PRIMER_INTERNAL_11_SELF_END_TH=0.00 PRIMER_LEFT_11_SELF_END_TH=5.82 PRIMER_RIGHT_11_SELF_END_TH=0.00 PRIMER_LEFT_11_HAIRPIN_TH=0.00 PRIMER_RIGHT_11_HAIRPIN_TH=43.86 PRIMER_INTERNAL_11_HAIRPIN_TH=41.60 PRIMER_LEFT_11_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_11_END_STABILITY=3.8500 PRIMER_RIGHT_11_END_STABILITY=3.5900 PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=7.8904 PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_12_PENALTY=1.179094 PRIMER_RIGHT_12_PENALTY=1.568623 PRIMER_INTERNAL_12_PENALTY=2.885177 PRIMER_LEFT_12_SEQUENCE=TTCGATCCCGACCCAAATCG PRIMER_RIGHT_12_SEQUENCE=GGATCGAATGGCCCACTAAT PRIMER_INTERNAL_12_SEQUENCE=GGGCCATTCGATCCCGACCCAA PRIMER_LEFT_12=72,20 PRIMER_RIGHT_12=79,20 PRIMER_INTERNAL_12=66,22 PRIMER_LEFT_12_TM=60.179 PRIMER_RIGHT_12_TM=57.431 PRIMER_INTERNAL_12_TM=60.885 PRIMER_LEFT_12_GC_PERCENT=55.000 PRIMER_RIGHT_12_GC_PERCENT=50.000 PRIMER_INTERNAL_12_GC_PERCENT=63.636 PRIMER_INTERNAL_12_SELF_ANY_TH=2.16 PRIMER_LEFT_12_SELF_ANY_TH=0.00 PRIMER_RIGHT_12_SELF_ANY_TH=0.00 PRIMER_INTERNAL_12_SELF_END_TH=0.00 PRIMER_LEFT_12_SELF_END_TH=0.00 PRIMER_RIGHT_12_SELF_END_TH=0.00 PRIMER_LEFT_12_HAIRPIN_TH=45.50 PRIMER_RIGHT_12_HAIRPIN_TH=0.00 PRIMER_INTERNAL_12_HAIRPIN_TH=41.60 PRIMER_LEFT_12_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_12_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA PRIMER_LEFT_12_END_STABILITY=3.3400 PRIMER_RIGHT_12_END_STABILITY=1.7300 PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=2.8344 PRIMER_LEFT_13_PENALTY=1.256117 PRIMER_RIGHT_13_PENALTY=1.673833 PRIMER_INTERNAL_13_PENALTY=3.114431 PRIMER_LEFT_13_SEQUENCE=ATTCGATCCCGACCCAAATC PRIMER_RIGHT_13_SEQUENCE=GTAGCTATCGATTTGGGTCGG PRIMER_INTERNAL_13_SEQUENCE=TAGTGGGCCATTCGATCCCGACC PRIMER_LEFT_13=71,20 PRIMER_RIGHT_13=99,21 PRIMER_INTERNAL_13=62,23 PRIMER_LEFT_13_TM=57.744 PRIMER_RIGHT_13_TM=58.326 PRIMER_INTERNAL_13_TM=60.114 PRIMER_LEFT_13_GC_PERCENT=50.000 PRIMER_RIGHT_13_GC_PERCENT=52.381 PRIMER_INTERNAL_13_GC_PERCENT=60.870 PRIMER_INTERNAL_13_SELF_ANY_TH=5.61 PRIMER_LEFT_13_SELF_ANY_TH=0.00 PRIMER_RIGHT_13_SELF_ANY_TH=0.00 PRIMER_INTERNAL_13_SELF_END_TH=0.00 PRIMER_LEFT_13_SELF_END_TH=0.00 PRIMER_RIGHT_13_SELF_END_TH=0.00 PRIMER_LEFT_13_HAIRPIN_TH=45.50 PRIMER_RIGHT_13_HAIRPIN_TH=41.74 PRIMER_INTERNAL_13_HAIRPIN_TH=41.60 PRIMER_LEFT_13_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_RIGHT_13_LIBRARY_MISPRIMING=11.00, MER4A a consensus PRIMER_LEFT_13_END_STABILITY=2.1700 PRIMER_RIGHT_13_END_STABILITY=4.7900 PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=0.0372 PRIMER_LEFT_14_PENALTY=1.316792 PRIMER_RIGHT_14_PENALTY=1.726673 PRIMER_INTERNAL_14_PENALTY=3.510595 PRIMER_LEFT_14_SEQUENCE=CGACCCAAATCGATAGCTACG PRIMER_RIGHT_14_SEQUENCE=TGCATCGTCAGTGTCATGTCT PRIMER_INTERNAL_14_SEQUENCE=GTGGGCCATTCGATCCCGACCC PRIMER_LEFT_14=80,21 PRIMER_RIGHT_14=30,21 PRIMER_INTERNAL_14=64,22 PRIMER_LEFT_14_TM=58.683 PRIMER_RIGHT_14_TM=59.727 PRIMER_INTERNAL_14_TM=61.511 PRIMER_LEFT_14_GC_PERCENT=52.381 PRIMER_RIGHT_14_GC_PERCENT=47.619 PRIMER_INTERNAL_14_GC_PERCENT=68.182 PRIMER_INTERNAL_14_SELF_ANY_TH=2.53 PRIMER_LEFT_14_SELF_ANY_TH=8.77 PRIMER_RIGHT_14_SELF_ANY_TH=1.94 PRIMER_INTERNAL_14_SELF_END_TH=0.25 PRIMER_LEFT_14_SELF_END_TH=8.77 PRIMER_RIGHT_14_SELF_END_TH=0.00 PRIMER_LEFT_14_HAIRPIN_TH=45.13 PRIMER_RIGHT_14_HAIRPIN_TH=0.00 PRIMER_INTERNAL_14_HAIRPIN_TH=41.60 PRIMER_LEFT_14_LIBRARY_MISPRIMING=10.00, MER4A a consensus PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_14_END_STABILITY=3.5100 PRIMER_RIGHT_14_END_STABILITY=3.4100 PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=4.9773 PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=6.6554 PRIMER_LEFT_15_PENALTY=1.383135 PRIMER_RIGHT_15_PENALTY=1.728621 PRIMER_INTERNAL_15_PENALTY=3.685489 PRIMER_LEFT_15_SEQUENCE=CCCGACCCAAATCGATAGCTA PRIMER_RIGHT_15_SEQUENCE=TATCGATTTGGGTCGGGATCG PRIMER_INTERNAL_15_SEQUENCE=GGCCATTCGATCCCGACCCAAAT PRIMER_LEFT_15=78,21 PRIMER_RIGHT_15=94,21 PRIMER_INTERNAL_15=67,23 PRIMER_LEFT_15_TM=59.383 PRIMER_RIGHT_15_TM=59.729 PRIMER_INTERNAL_15_TM=59.315 PRIMER_LEFT_15_GC_PERCENT=52.381 PRIMER_RIGHT_15_GC_PERCENT=52.381 PRIMER_INTERNAL_15_GC_PERCENT=56.522 PRIMER_INTERNAL_15_SELF_ANY_TH=0.00 PRIMER_LEFT_15_SELF_ANY_TH=0.00 PRIMER_RIGHT_15_SELF_ANY_TH=1.95 PRIMER_INTERNAL_15_SELF_END_TH=0.00 PRIMER_LEFT_15_SELF_END_TH=0.00 PRIMER_RIGHT_15_SELF_END_TH=0.00 PRIMER_LEFT_15_HAIRPIN_TH=45.13 PRIMER_RIGHT_15_HAIRPIN_TH=41.74 PRIMER_INTERNAL_15_HAIRPIN_TH=41.60 PRIMER_LEFT_15_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_15_END_STABILITY=3.3200 PRIMER_RIGHT_15_END_STABILITY=3.6900 PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=2.9487 PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=9.4327 PRIMER_LEFT_16_PENALTY=1.475039 PRIMER_RIGHT_16_PENALTY=1.914379 PRIMER_INTERNAL_16_PENALTY=3.807618 PRIMER_LEFT_16_SEQUENCE=CCATTCGATCCCGACCCAA PRIMER_RIGHT_16_SEQUENCE=GCTATCGATTTGGGTCGGG PRIMER_INTERNAL_16_SEQUENCE=TAGTGGGCCATTCGATCCCGAC PRIMER_LEFT_16=69,19 PRIMER_RIGHT_16=96,19 PRIMER_INTERNAL_16=62,22 PRIMER_LEFT_16_TM=59.475 PRIMER_RIGHT_16_TM=58.086 PRIMER_INTERNAL_16_TM=58.192 PRIMER_LEFT_16_GC_PERCENT=57.895 PRIMER_RIGHT_16_GC_PERCENT=57.895 PRIMER_INTERNAL_16_GC_PERCENT=59.091 PRIMER_INTERNAL_16_SELF_ANY_TH=3.57 PRIMER_LEFT_16_SELF_ANY_TH=0.00 PRIMER_RIGHT_16_SELF_ANY_TH=1.64 PRIMER_INTERNAL_16_SELF_END_TH=3.02 PRIMER_LEFT_16_SELF_END_TH=0.00 PRIMER_RIGHT_16_SELF_END_TH=0.00 PRIMER_LEFT_16_HAIRPIN_TH=45.50 PRIMER_RIGHT_16_HAIRPIN_TH=41.74 PRIMER_INTERNAL_16_HAIRPIN_TH=41.60 PRIMER_LEFT_16_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence PRIMER_RIGHT_16_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_LEFT_16_END_STABILITY=4.1200 PRIMER_RIGHT_16_END_STABILITY=5.1400 PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=5.1442 PRIMER_LEFT_17_PENALTY=1.559940 PRIMER_RIGHT_17_PENALTY=1.926077 PRIMER_INTERNAL_17_PENALTY=3.826170 PRIMER_LEFT_17_SEQUENCE=CGATCCCGACCCAAATCGA PRIMER_RIGHT_17_SEQUENCE=GGGATCGAATGGCCCACTAAT PRIMER_INTERNAL_17_SEQUENCE=GCCATTCGATCCCGACCCAAA PRIMER_LEFT_17=74,19 PRIMER_RIGHT_17=80,21 PRIMER_INTERNAL_17=68,21 PRIMER_LEFT_17_TM=59.560 PRIMER_RIGHT_17_TM=59.926 PRIMER_INTERNAL_17_TM=57.174 PRIMER_LEFT_17_GC_PERCENT=57.895 PRIMER_RIGHT_17_GC_PERCENT=52.381 PRIMER_INTERNAL_17_GC_PERCENT=57.143 PRIMER_INTERNAL_17_SELF_ANY_TH=0.00 PRIMER_LEFT_17_SELF_ANY_TH=0.00 PRIMER_RIGHT_17_SELF_ANY_TH=10.16 PRIMER_INTERNAL_17_SELF_END_TH=0.00 PRIMER_LEFT_17_SELF_END_TH=0.00 PRIMER_RIGHT_17_SELF_END_TH=0.00 PRIMER_LEFT_17_HAIRPIN_TH=45.13 PRIMER_RIGHT_17_HAIRPIN_TH=32.36 PRIMER_INTERNAL_17_HAIRPIN_TH=41.60 PRIMER_LEFT_17_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_RIGHT_17_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA PRIMER_LEFT_17_END_STABILITY=3.5900 PRIMER_RIGHT_17_END_STABILITY=1.7300 PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=1.1258 PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=12.3668 PRIMER_LEFT_18_PENALTY=1.559940 PRIMER_RIGHT_18_PENALTY=1.960819 PRIMER_INTERNAL_18_PENALTY=3.864865 PRIMER_LEFT_18_SEQUENCE=TCGATCCCGACCCAAATCG PRIMER_RIGHT_18_SEQUENCE=CTATCGATTTGGGTCGGGATC PRIMER_INTERNAL_18_SEQUENCE=ACACACACACACAGCACACAGGT PRIMER_LEFT_18=73,19 PRIMER_RIGHT_18=95,21 PRIMER_INTERNAL_18=37,23 PRIMER_LEFT_18_TM=59.560 PRIMER_RIGHT_18_TM=58.039 PRIMER_INTERNAL_18_TM=59.135 PRIMER_LEFT_18_GC_PERCENT=57.895 PRIMER_RIGHT_18_GC_PERCENT=52.381 PRIMER_INTERNAL_18_GC_PERCENT=52.174 PRIMER_INTERNAL_18_SELF_ANY_TH=0.00 PRIMER_LEFT_18_SELF_ANY_TH=0.00 PRIMER_RIGHT_18_SELF_ANY_TH=0.00 PRIMER_INTERNAL_18_SELF_END_TH=0.00 PRIMER_LEFT_18_SELF_END_TH=0.00 PRIMER_RIGHT_18_SELF_END_TH=0.00 PRIMER_LEFT_18_HAIRPIN_TH=40.30 PRIMER_RIGHT_18_HAIRPIN_TH=41.74 PRIMER_INTERNAL_18_HAIRPIN_TH=0.00 PRIMER_LEFT_18_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_18_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_18_END_STABILITY=3.3400 PRIMER_RIGHT_18_END_STABILITY=3.3600 PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=0.7760 PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=1.0131 PRIMER_LEFT_19_PENALTY=1.568623 PRIMER_RIGHT_19_PENALTY=1.997913 PRIMER_INTERNAL_19_PENALTY=3.881423 PRIMER_LEFT_19_SEQUENCE=ATTAGTGGGCCATTCGATCC PRIMER_RIGHT_19_SEQUENCE=GCTATCGATTTGGGTCGGGAT PRIMER_INTERNAL_19_SEQUENCE=TAGTGGGCCATTCGATCCCGA PRIMER_LEFT_19=60,20 PRIMER_RIGHT_19=96,21 PRIMER_INTERNAL_19=62,21 PRIMER_LEFT_19_TM=57.431 PRIMER_RIGHT_19_TM=59.998 PRIMER_INTERNAL_19_TM=57.119 PRIMER_LEFT_19_GC_PERCENT=50.000 PRIMER_RIGHT_19_GC_PERCENT=52.381 PRIMER_INTERNAL_19_GC_PERCENT=57.143 PRIMER_INTERNAL_19_SELF_ANY_TH=0.00 PRIMER_LEFT_19_SELF_ANY_TH=0.00 PRIMER_RIGHT_19_SELF_ANY_TH=1.64 PRIMER_INTERNAL_19_SELF_END_TH=0.00 PRIMER_LEFT_19_SELF_END_TH=0.00 PRIMER_RIGHT_19_SELF_END_TH=0.00 PRIMER_LEFT_19_HAIRPIN_TH=0.00 PRIMER_RIGHT_19_HAIRPIN_TH=41.74 PRIMER_INTERNAL_19_HAIRPIN_TH=41.96 PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence PRIMER_RIGHT_19_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_19_END_STABILITY=3.3600 PRIMER_RIGHT_19_END_STABILITY=3.8500 PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=2.1179 PRIMER_LEFT_20_PENALTY=1.673833 PRIMER_RIGHT_20_PENALTY=2.038681 PRIMER_INTERNAL_20_PENALTY=3.936624 PRIMER_LEFT_20_SEQUENCE=CCGACCCAAATCGATAGCTAC PRIMER_RIGHT_20_SEQUENCE=TCGATTTGGGTCGGGATCGA PRIMER_INTERNAL_20_SEQUENCE=TGGGCCATTCGATCCCGACCCA PRIMER_LEFT_20=79,21 PRIMER_RIGHT_20=92,20 PRIMER_INTERNAL_20=65,22 PRIMER_LEFT_20_TM=58.326 PRIMER_RIGHT_20_TM=61.039 PRIMER_INTERNAL_20_TM=61.937 PRIMER_LEFT_20_GC_PERCENT=52.381 PRIMER_RIGHT_20_GC_PERCENT=55.000 PRIMER_INTERNAL_20_GC_PERCENT=63.636 PRIMER_INTERNAL_20_SELF_ANY_TH=8.58 PRIMER_LEFT_20_SELF_ANY_TH=0.00 PRIMER_RIGHT_20_SELF_ANY_TH=5.62 PRIMER_INTERNAL_20_SELF_END_TH=6.38 PRIMER_LEFT_20_SELF_END_TH=0.00 PRIMER_RIGHT_20_SELF_END_TH=4.35 PRIMER_LEFT_20_HAIRPIN_TH=45.13 PRIMER_RIGHT_20_HAIRPIN_TH=43.86 PRIMER_INTERNAL_20_HAIRPIN_TH=42.67 PRIMER_LEFT_20_LIBRARY_MISPRIMING=11.00, MER4A a consensus PRIMER_RIGHT_20_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_20_END_STABILITY=3.5800 PRIMER_RIGHT_20_END_STABILITY=3.5900 PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=1.5945 PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_21_PENALTY=1.726673 PRIMER_RIGHT_21_PENALTY=2.150848 PRIMER_INTERNAL_21_PENALTY=3.956741 PRIMER_LEFT_21_SEQUENCE=AGACATGACACTGACGATGCA PRIMER_RIGHT_21_SEQUENCE=GCATCGTCAGTGTCATGTCTA PRIMER_INTERNAL_21_SEQUENCE=GGCCATTCGATCCCGACCC PRIMER_LEFT_21=10,21 PRIMER_RIGHT_21=29,21 PRIMER_INTERNAL_21=67,19 PRIMER_LEFT_21_TM=59.727 PRIMER_RIGHT_21_TM=57.849 PRIMER_INTERNAL_21_TM=57.043 PRIMER_LEFT_21_GC_PERCENT=47.619 PRIMER_RIGHT_21_GC_PERCENT=47.619 PRIMER_INTERNAL_21_GC_PERCENT=68.421 PRIMER_INTERNAL_21_SELF_ANY_TH=0.00 PRIMER_LEFT_21_SELF_ANY_TH=0.00 PRIMER_RIGHT_21_SELF_ANY_TH=0.00 PRIMER_INTERNAL_21_SELF_END_TH=0.00 PRIMER_LEFT_21_SELF_END_TH=0.00 PRIMER_RIGHT_21_SELF_END_TH=0.00 PRIMER_LEFT_21_HAIRPIN_TH=0.00 PRIMER_RIGHT_21_HAIRPIN_TH=0.00 PRIMER_INTERNAL_21_HAIRPIN_TH=41.60 PRIMER_LEFT_21_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_21_END_STABILITY=3.9600 PRIMER_RIGHT_21_END_STABILITY=2.5900 PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=5.0696 PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=9.0790 PRIMER_LEFT_22_PENALTY=1.728621 PRIMER_RIGHT_22_PENALTY=2.496898 PRIMER_INTERNAL_22_PENALTY=3.956741 PRIMER_LEFT_22_SEQUENCE=CGATCCCGACCCAAATCGATA PRIMER_RIGHT_22_SEQUENCE=GTCTGCATCGTCAGTGTCA PRIMER_INTERNAL_22_SEQUENCE=GGGCCATTCGATCCCGACC PRIMER_LEFT_22=74,21 PRIMER_RIGHT_22=33,19 PRIMER_INTERNAL_22=66,19 PRIMER_LEFT_22_TM=59.729 PRIMER_RIGHT_22_TM=57.503 PRIMER_INTERNAL_22_TM=57.043 PRIMER_LEFT_22_GC_PERCENT=52.381 PRIMER_RIGHT_22_GC_PERCENT=52.632 PRIMER_INTERNAL_22_GC_PERCENT=68.421 PRIMER_INTERNAL_22_SELF_ANY_TH=2.18 PRIMER_LEFT_22_SELF_ANY_TH=0.00 PRIMER_RIGHT_22_SELF_ANY_TH=0.00 PRIMER_INTERNAL_22_SELF_END_TH=0.00 PRIMER_LEFT_22_SELF_END_TH=0.00 PRIMER_RIGHT_22_SELF_END_TH=0.00 PRIMER_LEFT_22_HAIRPIN_TH=45.13 PRIMER_RIGHT_22_HAIRPIN_TH=0.00 PRIMER_INTERNAL_22_HAIRPIN_TH=41.60 PRIMER_LEFT_22_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_RIGHT_22_LIBRARY_MISPRIMING=12.00, reverse L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence PRIMER_LEFT_22_END_STABILITY=2.9200 PRIMER_RIGHT_22_END_STABILITY=3.5800 PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_23_PENALTY=1.914379 PRIMER_RIGHT_23_PENALTY=2.637998 PRIMER_INTERNAL_23_PENALTY=4.001118 PRIMER_LEFT_23_SEQUENCE=CCCGACCCAAATCGATAGC PRIMER_RIGHT_23_SEQUENCE=TCGTAGCTATCGATTTGGGTC PRIMER_INTERNAL_23_SEQUENCE=CACACACACACACAGCACACAGGT PRIMER_LEFT_23=78,19 PRIMER_RIGHT_23=101,21 PRIMER_INTERNAL_23=36,24 PRIMER_LEFT_23_TM=58.086 PRIMER_RIGHT_23_TM=57.362 PRIMER_INTERNAL_23_TM=60.001 PRIMER_LEFT_23_GC_PERCENT=57.895 PRIMER_RIGHT_23_GC_PERCENT=47.619 PRIMER_INTERNAL_23_GC_PERCENT=54.167 PRIMER_INTERNAL_23_SELF_ANY_TH=0.00 PRIMER_LEFT_23_SELF_ANY_TH=0.00 PRIMER_RIGHT_23_SELF_ANY_TH=14.37 PRIMER_INTERNAL_23_SELF_END_TH=0.00 PRIMER_LEFT_23_SELF_END_TH=0.00 PRIMER_RIGHT_23_SELF_END_TH=0.00 PRIMER_LEFT_23_HAIRPIN_TH=45.13 PRIMER_RIGHT_23_HAIRPIN_TH=0.00 PRIMER_INTERNAL_23_HAIRPIN_TH=0.00 PRIMER_LEFT_23_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR PRIMER_RIGHT_23_LIBRARY_MISPRIMING=11.00, MER4A a consensus PRIMER_LEFT_23_END_STABILITY=2.9700 PRIMER_RIGHT_23_END_STABILITY=4.4600 PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=7.9435 PRIMER_LEFT_24_PENALTY=1.926077 PRIMER_RIGHT_24_PENALTY=2.695830 PRIMER_INTERNAL_24_PENALTY=4.001118 PRIMER_LEFT_24_SEQUENCE=ATTAGTGGGCCATTCGATCCC PRIMER_RIGHT_24_SEQUENCE=GGATCGAATGGCCCACTAATA PRIMER_INTERNAL_24_SEQUENCE=ACACACACACACACAGCACACAGG PRIMER_LEFT_24=60,21 PRIMER_RIGHT_24=79,21 PRIMER_INTERNAL_24=35,24 PRIMER_LEFT_24_TM=59.926 PRIMER_RIGHT_24_TM=57.304 PRIMER_INTERNAL_24_TM=60.001 PRIMER_LEFT_24_GC_PERCENT=52.381 PRIMER_RIGHT_24_GC_PERCENT=47.619 PRIMER_INTERNAL_24_GC_PERCENT=54.167 PRIMER_INTERNAL_24_SELF_ANY_TH=0.00 PRIMER_LEFT_24_SELF_ANY_TH=5.82 PRIMER_RIGHT_24_SELF_ANY_TH=0.00 PRIMER_INTERNAL_24_SELF_END_TH=0.00 PRIMER_LEFT_24_SELF_END_TH=5.82 PRIMER_RIGHT_24_SELF_END_TH=0.00 PRIMER_LEFT_24_HAIRPIN_TH=0.00 PRIMER_RIGHT_24_HAIRPIN_TH=0.00 PRIMER_INTERNAL_24_HAIRPIN_TH=0.00 PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_24_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA PRIMER_LEFT_24_END_STABILITY=3.8500 PRIMER_RIGHT_24_END_STABILITY=0.9800 PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=5.0305 PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.8197 PRIMER_LEFT_25_PENALTY=1.960819 PRIMER_RIGHT_25_PENALTY=2.754380 PRIMER_INTERNAL_25_PENALTY=4.058186 PRIMER_LEFT_25_SEQUENCE=GATCCCGACCCAAATCGATAG PRIMER_RIGHT_25_SEQUENCE=CGGGATCGAATGGCCCACTA PRIMER_INTERNAL_25_SEQUENCE=GGCCATTCGATCCCGACCCAAATC PRIMER_LEFT_25=75,21 PRIMER_RIGHT_25=81,20 PRIMER_INTERNAL_25=67,24 PRIMER_LEFT_25_TM=58.039 PRIMER_RIGHT_25_TM=61.754 PRIMER_INTERNAL_25_TM=59.942 PRIMER_LEFT_25_GC_PERCENT=52.381 PRIMER_RIGHT_25_GC_PERCENT=60.000 PRIMER_INTERNAL_25_GC_PERCENT=58.333 PRIMER_INTERNAL_25_SELF_ANY_TH=0.00 PRIMER_LEFT_25_SELF_ANY_TH=0.00 PRIMER_RIGHT_25_SELF_ANY_TH=14.03 PRIMER_INTERNAL_25_SELF_END_TH=0.00 PRIMER_LEFT_25_SELF_END_TH=0.00 PRIMER_RIGHT_25_SELF_END_TH=0.00 PRIMER_LEFT_25_HAIRPIN_TH=45.13 PRIMER_RIGHT_25_HAIRPIN_TH=39.00 PRIMER_INTERNAL_25_HAIRPIN_TH=41.60 PRIMER_LEFT_25_LIBRARY_MISPRIMING=10.00, MSTa consensus sequence PRIMER_RIGHT_25_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_25_END_STABILITY=2.0800 PRIMER_RIGHT_25_END_STABILITY=2.7400 PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=9.7066 PRIMER_LEFT_26_PENALTY=1.997913 PRIMER_RIGHT_26_PENALTY=2.831715 PRIMER_INTERNAL_26_PENALTY=4.099823 PRIMER_LEFT_26_SEQUENCE=ATCCCGACCCAAATCGATAGC PRIMER_RIGHT_26_SEQUENCE=TCGTCAGTGTCATGTCTACTG PRIMER_INTERNAL_26_SEQUENCE=GGGCCATTCGATCCCGACCCAAA PRIMER_LEFT_26=76,21 PRIMER_RIGHT_26=26,21 PRIMER_INTERNAL_26=66,23 PRIMER_LEFT_26_TM=59.998 PRIMER_RIGHT_26_TM=57.168 PRIMER_INTERNAL_26_TM=61.100 PRIMER_LEFT_26_GC_PERCENT=52.381 PRIMER_RIGHT_26_GC_PERCENT=47.619 PRIMER_INTERNAL_26_GC_PERCENT=60.870 PRIMER_INTERNAL_26_SELF_ANY_TH=2.16 PRIMER_LEFT_26_SELF_ANY_TH=0.00 PRIMER_RIGHT_26_SELF_ANY_TH=15.66 PRIMER_INTERNAL_26_SELF_END_TH=0.00 PRIMER_LEFT_26_SELF_END_TH=0.00 PRIMER_RIGHT_26_SELF_END_TH=15.66 PRIMER_LEFT_26_HAIRPIN_TH=45.13 PRIMER_RIGHT_26_HAIRPIN_TH=0.00 PRIMER_INTERNAL_26_HAIRPIN_TH=41.60 PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR PRIMER_RIGHT_26_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_26_END_STABILITY=2.9700 PRIMER_RIGHT_26_END_STABILITY=2.7400 PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=24.9329 PRIMER_LEFT_27_PENALTY=2.038681 PRIMER_RIGHT_27_PENALTY=2.831715 PRIMER_INTERNAL_27_PENALTY=4.159384 PRIMER_LEFT_27_SEQUENCE=TCGATCCCGACCCAAATCGA PRIMER_RIGHT_27_SEQUENCE=CGTCAGTGTCATGTCTACTGA PRIMER_INTERNAL_27_SEQUENCE=CACACACACACAGCACACAGGT PRIMER_LEFT_27=73,20 PRIMER_RIGHT_27=25,21 PRIMER_INTERNAL_27=38,22 PRIMER_LEFT_27_TM=61.039 PRIMER_RIGHT_27_TM=57.168 PRIMER_INTERNAL_27_TM=57.841 PRIMER_LEFT_27_GC_PERCENT=55.000 PRIMER_RIGHT_27_GC_PERCENT=47.619 PRIMER_INTERNAL_27_GC_PERCENT=54.545 PRIMER_INTERNAL_27_SELF_ANY_TH=0.00 PRIMER_LEFT_27_SELF_ANY_TH=4.17 PRIMER_RIGHT_27_SELF_ANY_TH=19.25 PRIMER_INTERNAL_27_SELF_END_TH=0.00 PRIMER_LEFT_27_SELF_END_TH=2.87 PRIMER_RIGHT_27_SELF_END_TH=17.68 PRIMER_LEFT_27_HAIRPIN_TH=45.13 PRIMER_RIGHT_27_HAIRPIN_TH=0.00 PRIMER_INTERNAL_27_HAIRPIN_TH=0.00 PRIMER_LEFT_27_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER44C Nonautonomous DNA transposon PRIMER_LEFT_27_END_STABILITY=3.5900 PRIMER_RIGHT_27_END_STABILITY=3.4100 PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=3.8324 PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=25.4277 PRIMER_LEFT_28_PENALTY=2.150848 PRIMER_RIGHT_28_PENALTY=2.912899 PRIMER_INTERNAL_28_PENALTY=4.159384 PRIMER_LEFT_28_SEQUENCE=TAGACATGACACTGACGATGC PRIMER_RIGHT_28_SEQUENCE=TGCATCGTCAGTGTCATGT PRIMER_INTERNAL_28_SEQUENCE=ACACACACACACAGCACACAGG PRIMER_LEFT_28=9,21 PRIMER_RIGHT_28=30,19 PRIMER_INTERNAL_28=37,22 PRIMER_LEFT_28_TM=57.849 PRIMER_RIGHT_28_TM=57.087 PRIMER_INTERNAL_28_TM=57.841 PRIMER_LEFT_28_GC_PERCENT=47.619 PRIMER_RIGHT_28_GC_PERCENT=47.368 PRIMER_INTERNAL_28_GC_PERCENT=54.545 PRIMER_INTERNAL_28_SELF_ANY_TH=0.00 PRIMER_LEFT_28_SELF_ANY_TH=3.11 PRIMER_RIGHT_28_SELF_ANY_TH=3.35 PRIMER_INTERNAL_28_SELF_END_TH=0.00 PRIMER_LEFT_28_SELF_END_TH=0.00 PRIMER_RIGHT_28_SELF_END_TH=0.00 PRIMER_LEFT_28_HAIRPIN_TH=0.00 PRIMER_RIGHT_28_HAIRPIN_TH=0.00 PRIMER_INTERNAL_28_HAIRPIN_TH=0.00 PRIMER_LEFT_28_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_28_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_28_END_STABILITY=3.9100 PRIMER_RIGHT_28_END_STABILITY=3.2100 PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=12.1923 PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_29_PENALTY=2.469564 PRIMER_RIGHT_29_PENALTY=2.989104 PRIMER_INTERNAL_29_PENALTY=4.196497 PRIMER_LEFT_29_SEQUENCE=CATTCGATCCCGACCCAAA PRIMER_RIGHT_29_SEQUENCE=AGCTATCGATTTGGGTCGG PRIMER_INTERNAL_29_SEQUENCE=CACACACACACACAGCACACAGG PRIMER_LEFT_29=70,19 PRIMER_RIGHT_29=97,19 PRIMER_INTERNAL_29=36,23 PRIMER_LEFT_29_TM=57.530 PRIMER_RIGHT_29_TM=57.011 PRIMER_INTERNAL_29_TM=58.804 PRIMER_LEFT_29_GC_PERCENT=52.632 PRIMER_RIGHT_29_GC_PERCENT=52.632 PRIMER_INTERNAL_29_GC_PERCENT=56.522 PRIMER_INTERNAL_29_SELF_ANY_TH=0.00 PRIMER_LEFT_29_SELF_ANY_TH=0.00 PRIMER_RIGHT_29_SELF_ANY_TH=0.00 PRIMER_INTERNAL_29_SELF_END_TH=0.00 PRIMER_LEFT_29_SELF_END_TH=0.00 PRIMER_RIGHT_29_SELF_END_TH=0.00 PRIMER_LEFT_29_HAIRPIN_TH=45.50 PRIMER_RIGHT_29_HAIRPIN_TH=41.74 PRIMER_INTERNAL_29_HAIRPIN_TH=0.00 PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_LEFT_29_END_STABILITY=3.2800 PRIMER_RIGHT_29_END_STABILITY=4.7900 PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=8.0154 PRIMER_LEFT_30_PENALTY=2.476833 PRIMER_RIGHT_30_PENALTY=3.032680 PRIMER_INTERNAL_30_PENALTY=4.232604 PRIMER_LEFT_30_SEQUENCE=CCATTCGATCCCGACCCAAAT PRIMER_RIGHT_30_SEQUENCE=AGCTATCGATTTGGGTCGGGA PRIMER_INTERNAL_30_SEQUENCE=GCCATTCGATCCCGACCCAAATCG PRIMER_LEFT_30=69,21 PRIMER_RIGHT_30=97,21 PRIMER_INTERNAL_30=68,24 PRIMER_LEFT_30_TM=60.477 PRIMER_RIGHT_30_TM=61.033 PRIMER_INTERNAL_30_TM=60.233 PRIMER_LEFT_30_GC_PERCENT=52.381 PRIMER_RIGHT_30_GC_PERCENT=52.381 PRIMER_INTERNAL_30_GC_PERCENT=58.333 PRIMER_INTERNAL_30_SELF_ANY_TH=0.00 PRIMER_LEFT_30_SELF_ANY_TH=0.00 PRIMER_RIGHT_30_SELF_ANY_TH=0.00 PRIMER_INTERNAL_30_SELF_END_TH=0.00 PRIMER_LEFT_30_SELF_END_TH=0.00 PRIMER_RIGHT_30_SELF_END_TH=0.00 PRIMER_LEFT_30_HAIRPIN_TH=45.50 PRIMER_RIGHT_30_HAIRPIN_TH=41.74 PRIMER_INTERNAL_30_HAIRPIN_TH=41.60 PRIMER_LEFT_30_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_30_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_30_END_STABILITY=2.3200 PRIMER_RIGHT_30_END_STABILITY=5.1400 PRIMER_LEFT_30_TEMPLATE_MISPRIMING_TH=4.3679 PRIMER_RIGHT_30_TEMPLATE_MISPRIMING_TH=2.6099 PRIMER_LEFT_31_PENALTY=2.496898 PRIMER_RIGHT_31_PENALTY=3.367844 PRIMER_INTERNAL_31_PENALTY=4.351864 PRIMER_LEFT_31_SEQUENCE=TGACACTGACGATGCAGAC PRIMER_RIGHT_31_SEQUENCE=ATCGATTTGGGTCGGGATCGA PRIMER_INTERNAL_31_SEQUENCE=TTAGTGGGCCATTCGATCCCGACC PRIMER_LEFT_31=15,19 PRIMER_RIGHT_31=93,21 PRIMER_INTERNAL_31=61,24 PRIMER_LEFT_31_TM=57.503 PRIMER_RIGHT_31_TM=61.368 PRIMER_INTERNAL_31_TM=60.352 PRIMER_LEFT_31_GC_PERCENT=52.632 PRIMER_RIGHT_31_GC_PERCENT=52.381 PRIMER_INTERNAL_31_GC_PERCENT=58.333 PRIMER_INTERNAL_31_SELF_ANY_TH=5.61 PRIMER_LEFT_31_SELF_ANY_TH=4.19 PRIMER_RIGHT_31_SELF_ANY_TH=7.64 PRIMER_INTERNAL_31_SELF_END_TH=0.00 PRIMER_LEFT_31_SELF_END_TH=0.00 PRIMER_RIGHT_31_SELF_END_TH=7.64 PRIMER_LEFT_31_HAIRPIN_TH=0.00 PRIMER_RIGHT_31_HAIRPIN_TH=43.86 PRIMER_INTERNAL_31_HAIRPIN_TH=41.60 PRIMER_LEFT_31_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_31_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_31_END_STABILITY=3.5100 PRIMER_RIGHT_31_END_STABILITY=3.5900 PRIMER_LEFT_31_TEMPLATE_MISPRIMING_TH=8.4593 PRIMER_RIGHT_31_TEMPLATE_MISPRIMING_TH=1.6205 PRIMER_LEFT_32_PENALTY=2.540363 PRIMER_RIGHT_32_PENALTY=4.101796 PRIMER_INTERNAL_32_PENALTY=4.478673 PRIMER_LEFT_32_SEQUENCE=ATTCGATCCCGACCCAAATCG PRIMER_RIGHT_32_SEQUENCE=TCGGGATCGAATGGCCCAC PRIMER_INTERNAL_32_SEQUENCE=TTAGTGGGCCATTCGATCCCGAC PRIMER_LEFT_32=71,21 PRIMER_RIGHT_32=82,19 PRIMER_INTERNAL_32=61,23 PRIMER_LEFT_32_TM=60.540 PRIMER_RIGHT_32_TM=62.102 PRIMER_INTERNAL_32_TM=58.521 PRIMER_LEFT_32_GC_PERCENT=52.381 PRIMER_RIGHT_32_GC_PERCENT=63.158 PRIMER_INTERNAL_32_GC_PERCENT=56.522 PRIMER_INTERNAL_32_SELF_ANY_TH=3.57 PRIMER_LEFT_32_SELF_ANY_TH=0.00 PRIMER_RIGHT_32_SELF_ANY_TH=14.03 PRIMER_INTERNAL_32_SELF_END_TH=3.02 PRIMER_LEFT_32_SELF_END_TH=0.00 PRIMER_RIGHT_32_SELF_END_TH=0.00 PRIMER_LEFT_32_HAIRPIN_TH=45.50 PRIMER_RIGHT_32_HAIRPIN_TH=43.03 PRIMER_INTERNAL_32_HAIRPIN_TH=41.60 PRIMER_LEFT_32_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_32_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_32_END_STABILITY=3.3400 PRIMER_RIGHT_32_END_STABILITY=4.6100 PRIMER_LEFT_32_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_32_TEMPLATE_MISPRIMING_TH=11.0013 PRIMER_LEFT_33_PENALTY=2.637998 PRIMER_RIGHT_33_PENALTY=4.106986 PRIMER_INTERNAL_33_PENALTY=4.484274 PRIMER_LEFT_33_SEQUENCE=GACCCAAATCGATAGCTACGA PRIMER_RIGHT_33_SEQUENCE=CGGGATCGAATGGCCCACT PRIMER_INTERNAL_33_SEQUENCE=TTAGTGGGCCATTCGATCCCGA PRIMER_LEFT_33=81,21 PRIMER_RIGHT_33=81,19 PRIMER_INTERNAL_33=61,22 PRIMER_LEFT_33_TM=57.362 PRIMER_RIGHT_33_TM=62.107 PRIMER_INTERNAL_33_TM=57.516 PRIMER_LEFT_33_GC_PERCENT=47.619 PRIMER_RIGHT_33_GC_PERCENT=63.158 PRIMER_INTERNAL_33_GC_PERCENT=54.545 PRIMER_INTERNAL_33_SELF_ANY_TH=0.00 PRIMER_LEFT_33_SELF_ANY_TH=16.85 PRIMER_RIGHT_33_SELF_ANY_TH=14.03 PRIMER_INTERNAL_33_SELF_END_TH=0.00 PRIMER_LEFT_33_SELF_END_TH=16.85 PRIMER_RIGHT_33_SELF_END_TH=0.00 PRIMER_LEFT_33_HAIRPIN_TH=43.65 PRIMER_RIGHT_33_HAIRPIN_TH=39.00 PRIMER_INTERNAL_33_HAIRPIN_TH=41.96 PRIMER_LEFT_33_LIBRARY_MISPRIMING=11.00, MER4A a consensus PRIMER_RIGHT_33_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_33_END_STABILITY=3.4300 PRIMER_RIGHT_33_END_STABILITY=4.0000 PRIMER_LEFT_33_TEMPLATE_MISPRIMING_TH=2.4413 PRIMER_RIGHT_33_TEMPLATE_MISPRIMING_TH=10.5305 PRIMER_LEFT_34_PENALTY=2.695830 PRIMER_RIGHT_34_PENALTY=4.250296 PRIMER_INTERNAL_34_PENALTY=4.552303 PRIMER_LEFT_34_SEQUENCE=TATTAGTGGGCCATTCGATCC PRIMER_RIGHT_34_SEQUENCE=CGGGATCGAATGGCCCACTAA PRIMER_INTERNAL_34_SEQUENCE=ACACACACACACACAGCACACA PRIMER_LEFT_34=59,21 PRIMER_RIGHT_34=81,21 PRIMER_INTERNAL_34=35,22 PRIMER_LEFT_34_TM=57.304 PRIMER_RIGHT_34_TM=62.250 PRIMER_INTERNAL_34_TM=57.448 PRIMER_LEFT_34_GC_PERCENT=47.619 PRIMER_RIGHT_34_GC_PERCENT=57.143 PRIMER_INTERNAL_34_GC_PERCENT=50.000 PRIMER_INTERNAL_34_SELF_ANY_TH=0.00 PRIMER_LEFT_34_SELF_ANY_TH=0.00 PRIMER_RIGHT_34_SELF_ANY_TH=14.03 PRIMER_INTERNAL_34_SELF_END_TH=0.00 PRIMER_LEFT_34_SELF_END_TH=0.00 PRIMER_RIGHT_34_SELF_END_TH=0.00 PRIMER_LEFT_34_HAIRPIN_TH=0.00 PRIMER_RIGHT_34_HAIRPIN_TH=39.00 PRIMER_INTERNAL_34_HAIRPIN_TH=0.00 PRIMER_LEFT_34_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence PRIMER_RIGHT_34_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_34_END_STABILITY=3.3600 PRIMER_RIGHT_34_END_STABILITY=2.2400 PRIMER_LEFT_34_TEMPLATE_MISPRIMING_TH=1.4539 PRIMER_RIGHT_34_TEMPLATE_MISPRIMING_TH=10.9108 PRIMER_LEFT_35_PENALTY=2.754380 PRIMER_INTERNAL_35_PENALTY=4.639434 PRIMER_LEFT_35_SEQUENCE=TAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_35_SEQUENCE=GCCATTCGATCCCGACCCAAAT PRIMER_LEFT_35=62,20 PRIMER_INTERNAL_35=68,22 PRIMER_LEFT_35_TM=61.754 PRIMER_INTERNAL_35_TM=57.361 PRIMER_LEFT_35_GC_PERCENT=60.000 PRIMER_INTERNAL_35_GC_PERCENT=54.545 PRIMER_INTERNAL_35_SELF_ANY_TH=0.00 PRIMER_LEFT_35_SELF_ANY_TH=7.70 PRIMER_INTERNAL_35_SELF_END_TH=0.00 PRIMER_LEFT_35_SELF_END_TH=0.00 PRIMER_LEFT_35_HAIRPIN_TH=37.07 PRIMER_INTERNAL_35_HAIRPIN_TH=41.60 PRIMER_LEFT_35_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence PRIMER_LEFT_35_END_STABILITY=5.1400 PRIMER_LEFT_35_TEMPLATE_MISPRIMING_TH=6.4478 PRIMER_LEFT_36_PENALTY=2.772619 PRIMER_INTERNAL_36_PENALTY=4.809017 PRIMER_LEFT_36_SEQUENCE=TTCGATCCCGACCCAAATC PRIMER_INTERNAL_36_SEQUENCE=ACACACACACACACAGCACACAG PRIMER_LEFT_36=72,19 PRIMER_INTERNAL_36=35,23 PRIMER_LEFT_36_TM=57.227 PRIMER_INTERNAL_36_TM=58.191 PRIMER_LEFT_36_GC_PERCENT=52.632 PRIMER_INTERNAL_36_GC_PERCENT=52.174 PRIMER_INTERNAL_36_SELF_ANY_TH=0.00 PRIMER_LEFT_36_SELF_ANY_TH=0.00 PRIMER_INTERNAL_36_SELF_END_TH=0.00 PRIMER_LEFT_36_SELF_END_TH=0.00 PRIMER_LEFT_36_HAIRPIN_TH=45.50 PRIMER_INTERNAL_36_HAIRPIN_TH=0.00 PRIMER_LEFT_36_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_36_END_STABILITY=2.1700 PRIMER_LEFT_36_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_37_PENALTY=2.831715 PRIMER_INTERNAL_37_PENALTY=4.881095 PRIMER_LEFT_37_SEQUENCE=CAGTAGACATGACACTGACGA PRIMER_INTERNAL_37_SEQUENCE=GCCATTCGATCCCGACCCAAATC PRIMER_LEFT_37=6,21 PRIMER_INTERNAL_37=68,23 PRIMER_LEFT_37_TM=57.168 PRIMER_INTERNAL_37_TM=58.119 PRIMER_LEFT_37_GC_PERCENT=47.619 PRIMER_INTERNAL_37_GC_PERCENT=56.522 PRIMER_INTERNAL_37_SELF_ANY_TH=0.00 PRIMER_LEFT_37_SELF_ANY_TH=16.46 PRIMER_INTERNAL_37_SELF_END_TH=0.00 PRIMER_LEFT_37_SELF_END_TH=0.00 PRIMER_LEFT_37_HAIRPIN_TH=0.00 PRIMER_INTERNAL_37_HAIRPIN_TH=41.60 PRIMER_LEFT_37_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_37_END_STABILITY=4.2000 PRIMER_LEFT_37_TEMPLATE_MISPRIMING_TH=19.8783 PRIMER_LEFT_38_PENALTY=2.831715 PRIMER_INTERNAL_38_PENALTY=5.108025 PRIMER_LEFT_38_SEQUENCE=TCAGTAGACATGACACTGACG PRIMER_INTERNAL_38_SEQUENCE=TGGGCCATTCGATCCCGACCCAA PRIMER_LEFT_38=5,21 PRIMER_INTERNAL_38=65,23 PRIMER_LEFT_38_TM=57.168 PRIMER_INTERNAL_38_TM=62.108 PRIMER_LEFT_38_GC_PERCENT=47.619 PRIMER_INTERNAL_38_GC_PERCENT=60.870 PRIMER_INTERNAL_38_SELF_ANY_TH=8.58 PRIMER_LEFT_38_SELF_ANY_TH=20.80 PRIMER_INTERNAL_38_SELF_END_TH=0.00 PRIMER_LEFT_38_SELF_END_TH=6.93 PRIMER_LEFT_38_HAIRPIN_TH=0.00 PRIMER_INTERNAL_38_HAIRPIN_TH=41.29 PRIMER_LEFT_38_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_38_END_STABILITY=4.3500 PRIMER_LEFT_38_TEMPLATE_MISPRIMING_TH=6.9281 PRIMER_LEFT_39_PENALTY=2.912899 PRIMER_INTERNAL_39_PENALTY=5.111368 PRIMER_LEFT_39_SEQUENCE=ACATGACACTGACGATGCA PRIMER_INTERNAL_39_SEQUENCE=CCATTCGATCCCGACCCAAATCGA PRIMER_LEFT_39=12,19 PRIMER_INTERNAL_39=69,24 PRIMER_LEFT_39_TM=57.087 PRIMER_INTERNAL_39_TM=58.889 PRIMER_LEFT_39_GC_PERCENT=47.368 PRIMER_INTERNAL_39_GC_PERCENT=54.167 PRIMER_INTERNAL_39_SELF_ANY_TH=0.00 PRIMER_LEFT_39_SELF_ANY_TH=0.00 PRIMER_INTERNAL_39_SELF_END_TH=0.00 PRIMER_LEFT_39_SELF_END_TH=0.00 PRIMER_LEFT_39_HAIRPIN_TH=0.00 PRIMER_INTERNAL_39_HAIRPIN_TH=41.60 PRIMER_LEFT_39_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_39_END_STABILITY=3.9600 PRIMER_LEFT_39_TEMPLATE_MISPRIMING_TH=8.1705 PRIMER_LEFT_40_PENALTY=2.989104 PRIMER_INTERNAL_40_PENALTY=5.111455 PRIMER_LEFT_40_SEQUENCE=CCGACCCAAATCGATAGCT PRIMER_INTERNAL_40_SEQUENCE=CCATTCGATCCCGACCCAAATCG PRIMER_LEFT_40=79,19 PRIMER_INTERNAL_40=69,23 PRIMER_LEFT_40_TM=57.011 PRIMER_INTERNAL_40_TM=57.889 PRIMER_LEFT_40_GC_PERCENT=52.632 PRIMER_INTERNAL_40_GC_PERCENT=56.522 PRIMER_INTERNAL_40_SELF_ANY_TH=0.00 PRIMER_LEFT_40_SELF_ANY_TH=0.00 PRIMER_INTERNAL_40_SELF_END_TH=0.00 PRIMER_LEFT_40_SELF_END_TH=0.00 PRIMER_LEFT_40_HAIRPIN_TH=45.13 PRIMER_INTERNAL_40_HAIRPIN_TH=41.60 PRIMER_LEFT_40_LIBRARY_MISPRIMING=9.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR PRIMER_LEFT_40_END_STABILITY=3.3200 PRIMER_LEFT_40_TEMPLATE_MISPRIMING_TH=5.9515 PRIMER_LEFT_41_PENALTY=3.032680 PRIMER_INTERNAL_41_PENALTY=5.114088 PRIMER_LEFT_41_SEQUENCE=TCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_41_SEQUENCE=ACAGCACACAGGTATTAGTGGGCCA PRIMER_LEFT_41=77,21 PRIMER_INTERNAL_41=47,25 PRIMER_LEFT_41_TM=61.033 PRIMER_INTERNAL_41_TM=59.886 PRIMER_LEFT_41_GC_PERCENT=52.381 PRIMER_INTERNAL_41_GC_PERCENT=52.000 PRIMER_INTERNAL_41_SELF_ANY_TH=8.63 PRIMER_LEFT_41_SELF_ANY_TH=0.00 PRIMER_INTERNAL_41_SELF_END_TH=1.50 PRIMER_LEFT_41_SELF_END_TH=0.00 PRIMER_LEFT_41_HAIRPIN_TH=45.13 PRIMER_INTERNAL_41_HAIRPIN_TH=32.68 PRIMER_LEFT_41_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_LEFT_41_END_STABILITY=3.3200 PRIMER_LEFT_41_TEMPLATE_MISPRIMING_TH=1.5271 PRIMER_LEFT_42_PENALTY=3.367844 PRIMER_INTERNAL_42_PENALTY=5.122599 PRIMER_LEFT_42_SEQUENCE=TCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_42_SEQUENCE=GGGCCATTCGATCCCGACCCAAAT PRIMER_LEFT_42=73,21 PRIMER_INTERNAL_42=66,24 PRIMER_LEFT_42_TM=61.368 PRIMER_INTERNAL_42_TM=61.123 PRIMER_LEFT_42_GC_PERCENT=52.381 PRIMER_INTERNAL_42_GC_PERCENT=58.333 PRIMER_INTERNAL_42_SELF_ANY_TH=2.16 PRIMER_LEFT_42_SELF_ANY_TH=3.83 PRIMER_INTERNAL_42_SELF_END_TH=0.00 PRIMER_LEFT_42_SELF_END_TH=0.00 PRIMER_LEFT_42_HAIRPIN_TH=45.13 PRIMER_INTERNAL_42_HAIRPIN_TH=41.60 PRIMER_LEFT_42_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_LEFT_42_END_STABILITY=3.5900 PRIMER_LEFT_42_TEMPLATE_MISPRIMING_TH=5.3068 PRIMER_LEFT_43_PENALTY=3.568322 PRIMER_INTERNAL_43_PENALTY=5.268172 PRIMER_LEFT_43_SEQUENCE=TTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_43_SEQUENCE=AGCACACAGGTATTAGTGGGCCA PRIMER_LEFT_43=72,21 PRIMER_INTERNAL_43=49,23 PRIMER_LEFT_43_TM=61.568 PRIMER_INTERNAL_43_TM=57.732 PRIMER_LEFT_43_GC_PERCENT=52.381 PRIMER_INTERNAL_43_GC_PERCENT=52.174 PRIMER_INTERNAL_43_SELF_ANY_TH=8.63 PRIMER_LEFT_43_SELF_ANY_TH=4.17 PRIMER_INTERNAL_43_SELF_END_TH=1.50 PRIMER_LEFT_43_SELF_END_TH=3.06 PRIMER_LEFT_43_HAIRPIN_TH=45.50 PRIMER_INTERNAL_43_HAIRPIN_TH=32.68 PRIMER_LEFT_43_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_LEFT_43_END_STABILITY=3.5900 PRIMER_LEFT_43_TEMPLATE_MISPRIMING_TH=3.8324 PRIMER_LEFT_44_PENALTY=3.602746 PRIMER_INTERNAL_44_PENALTY=5.295331 PRIMER_LEFT_44_SEQUENCE=GCCATTCGATCCCGACCCAA PRIMER_INTERNAL_44_SEQUENCE=ACACACACACACAGCACACAGGTA PRIMER_LEFT_44=68,20 PRIMER_INTERNAL_44=37,24 PRIMER_LEFT_44_TM=62.603 PRIMER_INTERNAL_44_TM=58.705 PRIMER_LEFT_44_GC_PERCENT=60.000 PRIMER_INTERNAL_44_GC_PERCENT=50.000 PRIMER_INTERNAL_44_SELF_ANY_TH=0.00 PRIMER_LEFT_44_SELF_ANY_TH=0.00 PRIMER_INTERNAL_44_SELF_END_TH=0.00 PRIMER_LEFT_44_SELF_END_TH=0.00 PRIMER_LEFT_44_HAIRPIN_TH=45.50 PRIMER_INTERNAL_44_HAIRPIN_TH=0.00 PRIMER_LEFT_44_LIBRARY_MISPRIMING=10.00, MER4A a consensus PRIMER_LEFT_44_END_STABILITY=4.1200 PRIMER_LEFT_44_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_45_PENALTY=4.101796 PRIMER_INTERNAL_45_PENALTY=5.306369 PRIMER_LEFT_45_SEQUENCE=GCCATTCGATCCCGACCCA PRIMER_INTERNAL_45_SEQUENCE=CAGCACACAGGTATTAGTGGGCCA PRIMER_LEFT_45=68,19 PRIMER_INTERNAL_45=48,24 PRIMER_LEFT_45_TM=62.102 PRIMER_INTERNAL_45_TM=58.694 PRIMER_LEFT_45_GC_PERCENT=63.158 PRIMER_INTERNAL_45_GC_PERCENT=54.167 PRIMER_INTERNAL_45_SELF_ANY_TH=8.63 PRIMER_LEFT_45_SELF_ANY_TH=0.00 PRIMER_INTERNAL_45_SELF_END_TH=1.50 PRIMER_LEFT_45_SELF_END_TH=0.00 PRIMER_LEFT_45_HAIRPIN_TH=45.50 PRIMER_INTERNAL_45_HAIRPIN_TH=32.68 PRIMER_LEFT_45_LIBRARY_MISPRIMING=10.00, TCCCA Primate simple repeat pattern PRIMER_LEFT_45_END_STABILITY=4.5100 PRIMER_LEFT_45_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_46_PENALTY=4.101796 PRIMER_INTERNAL_46_PENALTY=5.307164 PRIMER_LEFT_46_SEQUENCE=TGGGCCATTCGATCCCGAC PRIMER_INTERNAL_46_SEQUENCE=ACAGCACACAGGTATTAGTGGGCC PRIMER_LEFT_46=65,19 PRIMER_INTERNAL_46=47,24 PRIMER_LEFT_46_TM=62.102 PRIMER_INTERNAL_46_TM=58.693 PRIMER_LEFT_46_GC_PERCENT=63.158 PRIMER_INTERNAL_46_GC_PERCENT=54.167 PRIMER_INTERNAL_46_SELF_ANY_TH=8.63 PRIMER_LEFT_46_SELF_ANY_TH=7.70 PRIMER_INTERNAL_46_SELF_END_TH=0.00 PRIMER_LEFT_46_SELF_END_TH=0.00 PRIMER_LEFT_46_HAIRPIN_TH=45.50 PRIMER_INTERNAL_46_HAIRPIN_TH=32.68 PRIMER_LEFT_46_LIBRARY_MISPRIMING=9.00, reverse LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat PRIMER_LEFT_46_END_STABILITY=4.7900 PRIMER_LEFT_46_TEMPLATE_MISPRIMING_TH=6.4764 PRIMER_LEFT_47_PENALTY=4.101796 PRIMER_INTERNAL_47_PENALTY=5.316271 PRIMER_LEFT_47_SEQUENCE=GTGGGCCATTCGATCCCGA PRIMER_INTERNAL_47_SEQUENCE=ATTAGTGGGCCATTCGATCCCGA PRIMER_LEFT_47=64,19 PRIMER_INTERNAL_47=60,23 PRIMER_LEFT_47_TM=62.102 PRIMER_INTERNAL_47_TM=57.684 PRIMER_LEFT_47_GC_PERCENT=63.158 PRIMER_INTERNAL_47_GC_PERCENT=52.174 PRIMER_INTERNAL_47_SELF_ANY_TH=0.00 PRIMER_LEFT_47_SELF_ANY_TH=5.82 PRIMER_INTERNAL_47_SELF_END_TH=0.00 PRIMER_LEFT_47_SELF_END_TH=0.00 PRIMER_LEFT_47_HAIRPIN_TH=43.96 PRIMER_INTERNAL_47_HAIRPIN_TH=41.96 PRIMER_LEFT_47_LIBRARY_MISPRIMING=9.00, MER1B Nonautonomous DNA transposon PRIMER_LEFT_47_END_STABILITY=5.1400 PRIMER_LEFT_47_TEMPLATE_MISPRIMING_TH=10.7677 PRIMER_LEFT_48_PENALTY=4.106986 PRIMER_INTERNAL_48_PENALTY=5.357946 PRIMER_LEFT_48_SEQUENCE=AGTGGGCCATTCGATCCCG PRIMER_INTERNAL_48_SEQUENCE=ATTAGTGGGCCATTCGATCCCGAC PRIMER_LEFT_48=63,19 PRIMER_INTERNAL_48=60,24 PRIMER_LEFT_48_TM=62.107 PRIMER_INTERNAL_48_TM=58.642 PRIMER_LEFT_48_GC_PERCENT=63.158 PRIMER_INTERNAL_48_GC_PERCENT=54.167 PRIMER_INTERNAL_48_SELF_ANY_TH=3.57 PRIMER_LEFT_48_SELF_ANY_TH=7.70 PRIMER_INTERNAL_48_SELF_END_TH=3.02 PRIMER_LEFT_48_SELF_END_TH=0.00 PRIMER_LEFT_48_HAIRPIN_TH=37.07 PRIMER_INTERNAL_48_HAIRPIN_TH=41.60 PRIMER_LEFT_48_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence PRIMER_LEFT_48_END_STABILITY=5.1400 PRIMER_LEFT_48_TEMPLATE_MISPRIMING_TH=6.4478 PRIMER_LEFT_49_PENALTY=4.250296 PRIMER_INTERNAL_49_PENALTY=5.401146 PRIMER_LEFT_49_SEQUENCE=TTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_49_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACC PRIMER_LEFT_49=61,21 PRIMER_INTERNAL_49=60,25 PRIMER_LEFT_49_TM=62.250 PRIMER_INTERNAL_49_TM=60.401 PRIMER_LEFT_49_GC_PERCENT=57.143 PRIMER_INTERNAL_49_GC_PERCENT=56.000 PRIMER_INTERNAL_49_SELF_ANY_TH=5.61 PRIMER_LEFT_49_SELF_ANY_TH=7.70 PRIMER_INTERNAL_49_SELF_END_TH=0.00 PRIMER_LEFT_49_SELF_END_TH=0.00 PRIMER_LEFT_49_HAIRPIN_TH=37.07 PRIMER_INTERNAL_49_HAIRPIN_TH=41.60 PRIMER_LEFT_49_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence PRIMER_LEFT_49_END_STABILITY=5.1400 PRIMER_LEFT_49_TEMPLATE_MISPRIMING_TH=6.4478 PRIMER_LEFT_50_PENALTY=4.826926 PRIMER_INTERNAL_50_PENALTY=5.439697 PRIMER_LEFT_50_SEQUENCE=GGCCATTCGATCCCGACCC PRIMER_INTERNAL_50_SEQUENCE=CACACACACACACAGCACACAGGTA PRIMER_LEFT_50=67,19 PRIMER_INTERNAL_50=36,25 PRIMER_LEFT_50_TM=62.827 PRIMER_INTERNAL_50_TM=59.560 PRIMER_LEFT_50_GC_PERCENT=68.421 PRIMER_INTERNAL_50_GC_PERCENT=52.000 PRIMER_INTERNAL_50_SELF_ANY_TH=0.00 PRIMER_LEFT_50_SELF_ANY_TH=0.00 PRIMER_INTERNAL_50_SELF_END_TH=0.00 PRIMER_LEFT_50_SELF_END_TH=0.00 PRIMER_LEFT_50_HAIRPIN_TH=45.50 PRIMER_INTERNAL_50_HAIRPIN_TH=0.00 PRIMER_LEFT_50_LIBRARY_MISPRIMING=9.00, TCCCA Primate simple repeat pattern PRIMER_LEFT_50_END_STABILITY=4.4600 PRIMER_LEFT_50_TEMPLATE_MISPRIMING_TH=3.6740 PRIMER_LEFT_51_PENALTY=4.826926 PRIMER_INTERNAL_51_PENALTY=5.444425 PRIMER_LEFT_51_SEQUENCE=GGGCCATTCGATCCCGACC PRIMER_INTERNAL_51_SEQUENCE=CACAGCACACAGGTATTAGTGGGCC PRIMER_LEFT_51=66,19 PRIMER_INTERNAL_51=46,25 PRIMER_LEFT_51_TM=62.827 PRIMER_INTERNAL_51_TM=59.556 PRIMER_LEFT_51_GC_PERCENT=68.421 PRIMER_INTERNAL_51_GC_PERCENT=56.000 PRIMER_INTERNAL_51_SELF_ANY_TH=8.63 PRIMER_LEFT_51_SELF_ANY_TH=4.39 PRIMER_INTERNAL_51_SELF_END_TH=0.00 PRIMER_LEFT_51_SELF_END_TH=0.00 PRIMER_LEFT_51_HAIRPIN_TH=45.50 PRIMER_INTERNAL_51_HAIRPIN_TH=32.68 PRIMER_LEFT_51_LIBRARY_MISPRIMING=9.00, HSATII Human satellite II DNA PRIMER_LEFT_51_END_STABILITY=4.7900 PRIMER_LEFT_51_TEMPLATE_MISPRIMING_TH=4.3247 PRIMER_INTERNAL_52_PENALTY=5.459011 PRIMER_INTERNAL_52_SEQUENCE=AGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_52=63,23 PRIMER_INTERNAL_52_TM=62.459 PRIMER_INTERNAL_52_GC_PERCENT=65.217 PRIMER_INTERNAL_52_SELF_ANY_TH=5.61 PRIMER_INTERNAL_52_SELF_END_TH=2.93 PRIMER_INTERNAL_52_HAIRPIN_TH=41.60 PRIMER_INTERNAL_53_PENALTY=5.514983 PRIMER_INTERNAL_53_SEQUENCE=TCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_53=73,24 PRIMER_INTERNAL_53_TM=58.485 PRIMER_INTERNAL_53_GC_PERCENT=54.167 PRIMER_INTERNAL_53_SELF_ANY_TH=0.30 PRIMER_INTERNAL_53_SELF_END_TH=0.00 PRIMER_INTERNAL_53_HAIRPIN_TH=41.28 PRIMER_INTERNAL_54_PENALTY=5.518553 PRIMER_INTERNAL_54_SEQUENCE=CGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_54=74,24 PRIMER_INTERNAL_54_TM=58.481 PRIMER_INTERNAL_54_GC_PERCENT=54.167 PRIMER_INTERNAL_54_SELF_ANY_TH=0.00 PRIMER_INTERNAL_54_SELF_END_TH=0.00 PRIMER_INTERNAL_54_HAIRPIN_TH=41.28 PRIMER_INTERNAL_55_PENALTY=5.523565 PRIMER_INTERNAL_55_SEQUENCE=CGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_55=74,23 PRIMER_INTERNAL_55_TM=57.476 PRIMER_INTERNAL_55_GC_PERCENT=56.522 PRIMER_INTERNAL_55_SELF_ANY_TH=0.00 PRIMER_INTERNAL_55_SELF_END_TH=0.00 PRIMER_INTERNAL_55_HAIRPIN_TH=41.28 PRIMER_INTERNAL_56_PENALTY=5.550131 PRIMER_INTERNAL_56_SEQUENCE=CACACACACACAGCACACAGGTA PRIMER_INTERNAL_56=38,23 PRIMER_INTERNAL_56_TM=57.450 PRIMER_INTERNAL_56_GC_PERCENT=52.174 PRIMER_INTERNAL_56_SELF_ANY_TH=0.00 PRIMER_INTERNAL_56_SELF_END_TH=0.00 PRIMER_INTERNAL_56_HAIRPIN_TH=0.00 PRIMER_INTERNAL_57_PENALTY=5.564155 PRIMER_INTERNAL_57_SEQUENCE=TCGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_57=73,25 PRIMER_INTERNAL_57_TM=59.436 PRIMER_INTERNAL_57_GC_PERCENT=52.000 PRIMER_INTERNAL_57_SELF_ANY_TH=0.30 PRIMER_INTERNAL_57_SELF_END_TH=0.00 PRIMER_INTERNAL_57_HAIRPIN_TH=41.28 PRIMER_INTERNAL_58_PENALTY=5.572672 PRIMER_INTERNAL_58_SEQUENCE=CAGCACACAGGTATTAGTGGGCC PRIMER_INTERNAL_58=48,23 PRIMER_INTERNAL_58_TM=57.427 PRIMER_INTERNAL_58_GC_PERCENT=56.522 PRIMER_INTERNAL_58_SELF_ANY_TH=8.63 PRIMER_INTERNAL_58_SELF_END_TH=0.00 PRIMER_INTERNAL_58_HAIRPIN_TH=32.68 PRIMER_INTERNAL_59_PENALTY=5.697915 PRIMER_INTERNAL_59_SEQUENCE=GTGGGCCATTCGATCCCGACCCA PRIMER_INTERNAL_59=64,23 PRIMER_INTERNAL_59_TM=62.698 PRIMER_INTERNAL_59_GC_PERCENT=65.217 PRIMER_INTERNAL_59_SELF_ANY_TH=8.58 PRIMER_INTERNAL_59_SELF_END_TH=6.40 PRIMER_INTERNAL_59_HAIRPIN_TH=42.67 PRIMER_INTERNAL_60_PENALTY=5.806699 PRIMER_INTERNAL_60_SEQUENCE=CCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_60=78,24 PRIMER_INTERNAL_60_TM=58.193 PRIMER_INTERNAL_60_GC_PERCENT=54.167 PRIMER_INTERNAL_60_SELF_ANY_TH=12.68 PRIMER_INTERNAL_60_SELF_END_TH=12.68 PRIMER_INTERNAL_60_HAIRPIN_TH=41.28 PRIMER_INTERNAL_61_PENALTY=5.806699 PRIMER_INTERNAL_61_SEQUENCE=TCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_61=77,24 PRIMER_INTERNAL_61_TM=58.193 PRIMER_INTERNAL_61_GC_PERCENT=54.167 PRIMER_INTERNAL_61_SELF_ANY_TH=5.17 PRIMER_INTERNAL_61_SELF_END_TH=5.17 PRIMER_INTERNAL_61_HAIRPIN_TH=41.28 PRIMER_INTERNAL_62_PENALTY=5.824076 PRIMER_INTERNAL_62_SEQUENCE=CCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_62=78,23 PRIMER_INTERNAL_62_TM=57.176 PRIMER_INTERNAL_62_GC_PERCENT=56.522 PRIMER_INTERNAL_62_SELF_ANY_TH=5.17 PRIMER_INTERNAL_62_SELF_END_TH=5.17 PRIMER_INTERNAL_62_HAIRPIN_TH=41.28 PRIMER_INTERNAL_63_PENALTY=5.846052 PRIMER_INTERNAL_63_SEQUENCE=TCCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_63=77,25 PRIMER_INTERNAL_63_TM=59.154 PRIMER_INTERNAL_63_GC_PERCENT=52.000 PRIMER_INTERNAL_63_SELF_ANY_TH=12.68 PRIMER_INTERNAL_63_SELF_END_TH=12.68 PRIMER_INTERNAL_63_HAIRPIN_TH=41.28 PRIMER_INTERNAL_64_PENALTY=5.892980 PRIMER_INTERNAL_64_SEQUENCE=TAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_64=62,24 PRIMER_INTERNAL_64_TM=61.893 PRIMER_INTERNAL_64_GC_PERCENT=62.500 PRIMER_INTERNAL_64_SELF_ANY_TH=5.61 PRIMER_INTERNAL_64_SELF_END_TH=2.93 PRIMER_INTERNAL_64_HAIRPIN_TH=41.60 PRIMER_INTERNAL_65_PENALTY=5.951461 PRIMER_INTERNAL_65_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_65=57,25 PRIMER_INTERNAL_65_TM=59.049 PRIMER_INTERNAL_65_GC_PERCENT=56.000 PRIMER_INTERNAL_65_SELF_ANY_TH=4.28 PRIMER_INTERNAL_65_SELF_END_TH=0.00 PRIMER_INTERNAL_65_HAIRPIN_TH=37.07 PRIMER_INTERNAL_66_PENALTY=5.980536 PRIMER_INTERNAL_66_SEQUENCE=CATTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_66=70,23 PRIMER_INTERNAL_66_TM=57.019 PRIMER_INTERNAL_66_GC_PERCENT=52.174 PRIMER_INTERNAL_66_SELF_ANY_TH=0.00 PRIMER_INTERNAL_66_SELF_END_TH=0.00 PRIMER_INTERNAL_66_HAIRPIN_TH=41.60 PRIMER_INTERNAL_67_PENALTY=6.009338 PRIMER_INTERNAL_67_SEQUENCE=CCATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_67=69,25 PRIMER_INTERNAL_67_TM=58.991 PRIMER_INTERNAL_67_GC_PERCENT=52.000 PRIMER_INTERNAL_67_SELF_ANY_TH=0.00 PRIMER_INTERNAL_67_SELF_END_TH=0.00 PRIMER_INTERNAL_67_HAIRPIN_TH=41.60 PRIMER_INTERNAL_68_PENALTY=6.015417 PRIMER_INTERNAL_68_SEQUENCE=ACACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_68=45,25 PRIMER_INTERNAL_68_TM=58.985 PRIMER_INTERNAL_68_GC_PERCENT=52.000 PRIMER_INTERNAL_68_SELF_ANY_TH=5.21 PRIMER_INTERNAL_68_SELF_END_TH=5.21 PRIMER_INTERNAL_68_HAIRPIN_TH=33.51 PRIMER_INTERNAL_69_PENALTY=6.046206 PRIMER_INTERNAL_69_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACC PRIMER_INTERNAL_69=59,26 PRIMER_INTERNAL_69_TM=59.954 PRIMER_INTERNAL_69_GC_PERCENT=53.846 PRIMER_INTERNAL_69_SELF_ANY_TH=5.61 PRIMER_INTERNAL_69_SELF_END_TH=0.00 PRIMER_INTERNAL_69_HAIRPIN_TH=41.60 PRIMER_INTERNAL_70_PENALTY=6.046206 PRIMER_INTERNAL_70_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_70=57,26 PRIMER_INTERNAL_70_TM=59.954 PRIMER_INTERNAL_70_GC_PERCENT=53.846 PRIMER_INTERNAL_70_SELF_ANY_TH=0.76 PRIMER_INTERNAL_70_SELF_END_TH=0.00 PRIMER_INTERNAL_70_HAIRPIN_TH=41.96 PRIMER_INTERNAL_71_PENALTY=6.046659 PRIMER_INTERNAL_71_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_71=56,26 PRIMER_INTERNAL_71_TM=59.953 PRIMER_INTERNAL_71_GC_PERCENT=53.846 PRIMER_INTERNAL_71_SELF_ANY_TH=4.31 PRIMER_INTERNAL_71_SELF_END_TH=0.00 PRIMER_INTERNAL_71_HAIRPIN_TH=37.07 PRIMER_INTERNAL_72_PENALTY=6.049301 PRIMER_INTERNAL_72_SEQUENCE=ACAGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_72=47,26 PRIMER_INTERNAL_72_TM=59.951 PRIMER_INTERNAL_72_GC_PERCENT=50.000 PRIMER_INTERNAL_72_SELF_ANY_TH=8.63 PRIMER_INTERNAL_72_SELF_END_TH=0.00 PRIMER_INTERNAL_72_HAIRPIN_TH=32.68 PRIMER_INTERNAL_73_PENALTY=6.095821 PRIMER_INTERNAL_73_SEQUENCE=GCACACAGGTATTAGTGGGCCATTCG PRIMER_INTERNAL_73=50,26 PRIMER_INTERNAL_73_TM=59.904 PRIMER_INTERNAL_73_GC_PERCENT=53.846 PRIMER_INTERNAL_73_SELF_ANY_TH=2.67 PRIMER_INTERNAL_73_SELF_END_TH=0.00 PRIMER_INTERNAL_73_HAIRPIN_TH=28.81 PRIMER_INTERNAL_74_PENALTY=6.114152 PRIMER_INTERNAL_74_SEQUENCE=AGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_74=49,24 PRIMER_INTERNAL_74_TM=57.886 PRIMER_INTERNAL_74_GC_PERCENT=50.000 PRIMER_INTERNAL_74_SELF_ANY_TH=8.63 PRIMER_INTERNAL_74_SELF_END_TH=0.00 PRIMER_INTERNAL_74_HAIRPIN_TH=32.68 PRIMER_INTERNAL_75_PENALTY=6.125318 PRIMER_INTERNAL_75_SEQUENCE=GCCATTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_75=68,25 PRIMER_INTERNAL_75_TM=61.125 PRIMER_INTERNAL_75_GC_PERCENT=56.000 PRIMER_INTERNAL_75_SELF_ANY_TH=0.00 PRIMER_INTERNAL_75_SELF_END_TH=0.00 PRIMER_INTERNAL_75_HAIRPIN_TH=41.60 PRIMER_INTERNAL_76_PENALTY=6.131527 PRIMER_INTERNAL_76_SEQUENCE=ACACACACACACACAGCACACAGGT PRIMER_INTERNAL_76=35,25 PRIMER_INTERNAL_76_TM=61.132 PRIMER_INTERNAL_76_GC_PERCENT=52.000 PRIMER_INTERNAL_76_SELF_ANY_TH=0.00 PRIMER_INTERNAL_76_SELF_END_TH=0.00 PRIMER_INTERNAL_76_HAIRPIN_TH=0.00 PRIMER_INTERNAL_77_PENALTY=6.186005 PRIMER_INTERNAL_77_SEQUENCE=ACACACACACACAGCACACAGGTAT PRIMER_INTERNAL_77=37,25 PRIMER_INTERNAL_77_TM=58.814 PRIMER_INTERNAL_77_GC_PERCENT=48.000 PRIMER_INTERNAL_77_SELF_ANY_TH=0.00 PRIMER_INTERNAL_77_SELF_END_TH=0.00 PRIMER_INTERNAL_77_HAIRPIN_TH=0.00 PRIMER_INTERNAL_78_PENALTY=6.195851 PRIMER_INTERNAL_78_SEQUENCE=CAGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_78=48,25 PRIMER_INTERNAL_78_TM=58.804 PRIMER_INTERNAL_78_GC_PERCENT=52.000 PRIMER_INTERNAL_78_SELF_ANY_TH=8.63 PRIMER_INTERNAL_78_SELF_END_TH=0.00 PRIMER_INTERNAL_78_HAIRPIN_TH=32.68 PRIMER_INTERNAL_79_PENALTY=6.203782 PRIMER_INTERNAL_79_SEQUENCE=CACACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_79=44,26 PRIMER_INTERNAL_79_TM=59.796 PRIMER_INTERNAL_79_GC_PERCENT=53.846 PRIMER_INTERNAL_79_SELF_ANY_TH=5.21 PRIMER_INTERNAL_79_SELF_END_TH=5.21 PRIMER_INTERNAL_79_HAIRPIN_TH=33.51 PRIMER_INTERNAL_80_PENALTY=6.214266 PRIMER_INTERNAL_80_SEQUENCE=CACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_80=46,24 PRIMER_INTERNAL_80_TM=57.786 PRIMER_INTERNAL_80_GC_PERCENT=54.167 PRIMER_INTERNAL_80_SELF_ANY_TH=5.21 PRIMER_INTERNAL_80_SELF_END_TH=5.21 PRIMER_INTERNAL_80_HAIRPIN_TH=33.51 PRIMER_INTERNAL_81_PENALTY=6.225896 PRIMER_INTERNAL_81_SEQUENCE=TTCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_81=72,25 PRIMER_INTERNAL_81_TM=58.774 PRIMER_INTERNAL_81_GC_PERCENT=52.000 PRIMER_INTERNAL_81_SELF_ANY_TH=0.00 PRIMER_INTERNAL_81_SELF_END_TH=0.00 PRIMER_INTERNAL_81_HAIRPIN_TH=41.60 PRIMER_INTERNAL_82_PENALTY=6.265114 PRIMER_INTERNAL_82_SEQUENCE=TGGGCCATTCGATCCCGACCCAAA PRIMER_INTERNAL_82=65,24 PRIMER_INTERNAL_82_TM=62.265 PRIMER_INTERNAL_82_GC_PERCENT=58.333 PRIMER_INTERNAL_82_SELF_ANY_TH=8.58 PRIMER_INTERNAL_82_SELF_END_TH=0.00 PRIMER_INTERNAL_82_HAIRPIN_TH=41.29 PRIMER_INTERNAL_83_PENALTY=6.318558 PRIMER_INTERNAL_83_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_83=72,26 PRIMER_INTERNAL_83_TM=59.681 PRIMER_INTERNAL_83_GC_PERCENT=50.000 PRIMER_INTERNAL_83_SELF_ANY_TH=0.00 PRIMER_INTERNAL_83_SELF_END_TH=0.00 PRIMER_INTERNAL_83_HAIRPIN_TH=41.60 PRIMER_INTERNAL_84_PENALTY=6.365515 PRIMER_INTERNAL_84_SEQUENCE=CACACACACACACAGCACACAGGTAT PRIMER_INTERNAL_84=36,26 PRIMER_INTERNAL_84_TM=59.634 PRIMER_INTERNAL_84_GC_PERCENT=50.000 PRIMER_INTERNAL_84_SELF_ANY_TH=0.00 PRIMER_INTERNAL_84_SELF_END_TH=0.00 PRIMER_INTERNAL_84_HAIRPIN_TH=0.00 PRIMER_INTERNAL_85_PENALTY=6.390437 PRIMER_INTERNAL_85_SEQUENCE=CACACACACACAGCACACAGGTAT PRIMER_INTERNAL_85=38,24 PRIMER_INTERNAL_85_TM=57.610 PRIMER_INTERNAL_85_GC_PERCENT=50.000 PRIMER_INTERNAL_85_SELF_ANY_TH=0.00 PRIMER_INTERNAL_85_SELF_END_TH=0.00 PRIMER_INTERNAL_85_HAIRPIN_TH=0.00 PRIMER_INTERNAL_86_PENALTY=6.635581 PRIMER_INTERNAL_86_SEQUENCE=GGGCCATTCGATCCCGACCCAAATC PRIMER_INTERNAL_86=66,25 PRIMER_INTERNAL_86_TM=61.636 PRIMER_INTERNAL_86_GC_PERCENT=60.000 PRIMER_INTERNAL_86_SELF_ANY_TH=2.16 PRIMER_INTERNAL_86_SELF_END_TH=0.00 PRIMER_INTERNAL_86_HAIRPIN_TH=41.60 PRIMER_INTERNAL_87_PENALTY=6.661393 PRIMER_INTERNAL_87_SEQUENCE=ACACACACACACACAGCACACAGGTA PRIMER_INTERNAL_87=35,26 PRIMER_INTERNAL_87_TM=60.661 PRIMER_INTERNAL_87_GC_PERCENT=50.000 PRIMER_INTERNAL_87_SELF_ANY_TH=0.00 PRIMER_INTERNAL_87_SELF_END_TH=0.00 PRIMER_INTERNAL_87_HAIRPIN_TH=0.00 PRIMER_INTERNAL_88_PENALTY=6.664685 PRIMER_INTERNAL_88_SEQUENCE=CACAGCACACAGGTATTAGTGGGCCA PRIMER_INTERNAL_88=46,26 PRIMER_INTERNAL_88_TM=60.665 PRIMER_INTERNAL_88_GC_PERCENT=53.846 PRIMER_INTERNAL_88_SELF_ANY_TH=8.63 PRIMER_INTERNAL_88_SELF_END_TH=1.50 PRIMER_INTERNAL_88_HAIRPIN_TH=32.68 PRIMER_INTERNAL_89_PENALTY=6.664922 PRIMER_INTERNAL_89_SEQUENCE=ACACAGCACACAGGTATTAGTGGGCC PRIMER_INTERNAL_89=45,26 PRIMER_INTERNAL_89_TM=60.665 PRIMER_INTERNAL_89_GC_PERCENT=53.846 PRIMER_INTERNAL_89_SELF_ANY_TH=8.63 PRIMER_INTERNAL_89_SELF_END_TH=0.00 PRIMER_INTERNAL_89_HAIRPIN_TH=32.68 PRIMER_INTERNAL_90_PENALTY=6.679783 PRIMER_INTERNAL_90_SEQUENCE=CCCGACCCAAATCGATAGCTACGAT PRIMER_INTERNAL_90=78,25 PRIMER_INTERNAL_90_TM=58.320 PRIMER_INTERNAL_90_GC_PERCENT=52.000 PRIMER_INTERNAL_90_SELF_ANY_TH=14.48 PRIMER_INTERNAL_90_SELF_END_TH=14.48 PRIMER_INTERNAL_90_HAIRPIN_TH=46.54 PRIMER_INTERNAL_91_PENALTY=6.679783 PRIMER_INTERNAL_91_SEQUENCE=ATCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_91=76,25 PRIMER_INTERNAL_91_TM=58.320 PRIMER_INTERNAL_91_GC_PERCENT=52.000 PRIMER_INTERNAL_91_SELF_ANY_TH=5.17 PRIMER_INTERNAL_91_SELF_END_TH=5.17 PRIMER_INTERNAL_91_HAIRPIN_TH=41.28 PRIMER_INTERNAL_92_PENALTY=6.695664 PRIMER_INTERNAL_92_SEQUENCE=TATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_92=59,24 PRIMER_INTERNAL_92_TM=57.304 PRIMER_INTERNAL_92_GC_PERCENT=50.000 PRIMER_INTERNAL_92_SELF_ANY_TH=0.00 PRIMER_INTERNAL_92_SELF_END_TH=0.00 PRIMER_INTERNAL_92_HAIRPIN_TH=41.96 PRIMER_INTERNAL_93_PENALTY=6.741797 PRIMER_INTERNAL_93_SEQUENCE=GTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_93=58,24 PRIMER_INTERNAL_93_TM=57.258 PRIMER_INTERNAL_93_GC_PERCENT=54.167 PRIMER_INTERNAL_93_SELF_ANY_TH=4.28 PRIMER_INTERNAL_93_SELF_END_TH=0.00 PRIMER_INTERNAL_93_HAIRPIN_TH=37.07 PRIMER_INTERNAL_94_PENALTY=6.754240 PRIMER_INTERNAL_94_SEQUENCE=TATTAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_94=59,25 PRIMER_INTERNAL_94_TM=58.246 PRIMER_INTERNAL_94_GC_PERCENT=52.000 PRIMER_INTERNAL_94_SELF_ANY_TH=3.57 PRIMER_INTERNAL_94_SELF_END_TH=3.02 PRIMER_INTERNAL_94_HAIRPIN_TH=41.60 PRIMER_INTERNAL_95_PENALTY=6.754240 PRIMER_INTERNAL_95_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_95=58,25 PRIMER_INTERNAL_95_TM=58.246 PRIMER_INTERNAL_95_GC_PERCENT=52.000 PRIMER_INTERNAL_95_SELF_ANY_TH=0.00 PRIMER_INTERNAL_95_SELF_END_TH=0.00 PRIMER_INTERNAL_95_HAIRPIN_TH=41.96 PRIMER_INTERNAL_96_PENALTY=6.756422 PRIMER_INTERNAL_96_SEQUENCE=TCCCGACCCAAATCGATAGCTACGAT PRIMER_INTERNAL_96=77,26 PRIMER_INTERNAL_96_TM=59.244 PRIMER_INTERNAL_96_GC_PERCENT=50.000 PRIMER_INTERNAL_96_SELF_ANY_TH=14.48 PRIMER_INTERNAL_96_SELF_END_TH=14.48 PRIMER_INTERNAL_96_HAIRPIN_TH=46.54 PRIMER_INTERNAL_97_PENALTY=6.756422 PRIMER_INTERNAL_97_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_97=76,26 PRIMER_INTERNAL_97_TM=59.244 PRIMER_INTERNAL_97_GC_PERCENT=50.000 PRIMER_INTERNAL_97_SELF_ANY_TH=12.68 PRIMER_INTERNAL_97_SELF_END_TH=12.68 PRIMER_INTERNAL_97_HAIRPIN_TH=41.28 PRIMER_INTERNAL_98_PENALTY=6.791532 PRIMER_INTERNAL_98_SEQUENCE=AGCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_98=49,25 PRIMER_INTERNAL_98_TM=58.208 PRIMER_INTERNAL_98_GC_PERCENT=48.000 PRIMER_INTERNAL_98_SELF_ANY_TH=8.63 PRIMER_INTERNAL_98_SELF_END_TH=0.00 PRIMER_INTERNAL_98_HAIRPIN_TH=32.68 PRIMER_INTERNAL_99_PENALTY=6.793369 PRIMER_INTERNAL_99_SEQUENCE=GCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_99=50,24 PRIMER_INTERNAL_99_TM=57.207 PRIMER_INTERNAL_99_GC_PERCENT=50.000 PRIMER_INTERNAL_99_SELF_ANY_TH=2.67 PRIMER_INTERNAL_99_SELF_END_TH=0.00 PRIMER_INTERNAL_99_HAIRPIN_TH=28.81 PRIMER_INTERNAL_100_PENALTY=6.804314 PRIMER_INTERNAL_100_SEQUENCE=CATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_100=70,24 PRIMER_INTERNAL_100_TM=57.196 PRIMER_INTERNAL_100_GC_PERCENT=50.000 PRIMER_INTERNAL_100_SELF_ANY_TH=0.00 PRIMER_INTERNAL_100_SELF_END_TH=0.00 PRIMER_INTERNAL_100_HAIRPIN_TH=41.60 PRIMER_INTERNAL_101_PENALTY=6.828710 PRIMER_INTERNAL_101_SEQUENCE=GTGGGCCATTCGATCCCGACCCAA PRIMER_INTERNAL_101=64,24 PRIMER_INTERNAL_101_TM=62.829 PRIMER_INTERNAL_101_GC_PERCENT=62.500 PRIMER_INTERNAL_101_SELF_ANY_TH=8.58 PRIMER_INTERNAL_101_SELF_END_TH=0.00 PRIMER_INTERNAL_101_HAIRPIN_TH=43.79 PRIMER_INTERNAL_102_PENALTY=6.841708 PRIMER_INTERNAL_102_SEQUENCE=CCCGACCCAAATCGATAGCTACGATG PRIMER_INTERNAL_102=78,26 PRIMER_INTERNAL_102_TM=59.158 PRIMER_INTERNAL_102_GC_PERCENT=53.846 PRIMER_INTERNAL_102_SELF_ANY_TH=5.23 PRIMER_INTERNAL_102_SELF_END_TH=0.00 PRIMER_INTERNAL_102_HAIRPIN_TH=43.02 PRIMER_INTERNAL_103_PENALTY=6.895596 PRIMER_INTERNAL_103_SEQUENCE=GGCCATTCGATCCCGACCCAAATCG PRIMER_INTERNAL_103=67,25 PRIMER_INTERNAL_103_TM=61.896 PRIMER_INTERNAL_103_GC_PERCENT=60.000 PRIMER_INTERNAL_103_SELF_ANY_TH=0.00 PRIMER_INTERNAL_103_SELF_END_TH=0.00 PRIMER_INTERNAL_103_HAIRPIN_TH=41.60 PRIMER_INTERNAL_104_PENALTY=6.905174 PRIMER_INTERNAL_104_SEQUENCE=CGATCCCGACCCAAATCGATAGCTA PRIMER_INTERNAL_104=74,25 PRIMER_INTERNAL_104_TM=58.095 PRIMER_INTERNAL_104_GC_PERCENT=52.000 PRIMER_INTERNAL_104_SELF_ANY_TH=0.00 PRIMER_INTERNAL_104_SELF_END_TH=0.00 PRIMER_INTERNAL_104_HAIRPIN_TH=41.28 PRIMER_INTERNAL_105_PENALTY=6.905408 PRIMER_INTERNAL_105_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_105=58,26 PRIMER_INTERNAL_105_TM=59.095 PRIMER_INTERNAL_105_GC_PERCENT=53.846 PRIMER_INTERNAL_105_SELF_ANY_TH=3.57 PRIMER_INTERNAL_105_SELF_END_TH=3.02 PRIMER_INTERNAL_105_HAIRPIN_TH=41.60 PRIMER_INTERNAL_106_PENALTY=6.917157 PRIMER_INTERNAL_106_SEQUENCE=ACACACACACACAGCACACAGGTATT PRIMER_INTERNAL_106=37,26 PRIMER_INTERNAL_106_TM=59.083 PRIMER_INTERNAL_106_GC_PERCENT=46.154 PRIMER_INTERNAL_106_SELF_ANY_TH=0.00 PRIMER_INTERNAL_106_SELF_END_TH=0.00 PRIMER_INTERNAL_106_HAIRPIN_TH=0.00 PRIMER_INTERNAL_107_PENALTY=6.924766 PRIMER_INTERNAL_107_SEQUENCE=CAGCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_107=48,26 PRIMER_INTERNAL_107_TM=59.075 PRIMER_INTERNAL_107_GC_PERCENT=50.000 PRIMER_INTERNAL_107_SELF_ANY_TH=8.63 PRIMER_INTERNAL_107_SELF_END_TH=0.00 PRIMER_INTERNAL_107_HAIRPIN_TH=32.68 PRIMER_INTERNAL_108_PENALTY=6.956454 PRIMER_INTERNAL_108_SEQUENCE=AGACATGACACTGACGATGCAGAC PRIMER_INTERNAL_108=10,24 PRIMER_INTERNAL_108_TM=57.044 PRIMER_INTERNAL_108_GC_PERCENT=50.000 PRIMER_INTERNAL_108_SELF_ANY_TH=1.44 PRIMER_INTERNAL_108_SELF_END_TH=0.00 PRIMER_INTERNAL_108_HAIRPIN_TH=0.00 PRIMER_INTERNAL_109_PENALTY=6.975446 PRIMER_INTERNAL_109_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTA PRIMER_INTERNAL_109=73,26 PRIMER_INTERNAL_109_TM=59.025 PRIMER_INTERNAL_109_GC_PERCENT=50.000 PRIMER_INTERNAL_109_SELF_ANY_TH=0.30 PRIMER_INTERNAL_109_SELF_END_TH=0.00 PRIMER_INTERNAL_109_HAIRPIN_TH=41.28 PRIMER_INTERNAL_110_PENALTY=7.015932 PRIMER_INTERNAL_110_SEQUENCE=CCCGACCCAAATCGATAGCTACGATGA PRIMER_INTERNAL_110=78,27 PRIMER_INTERNAL_110_TM=60.016 PRIMER_INTERNAL_110_GC_PERCENT=51.852 PRIMER_INTERNAL_110_SELF_ANY_TH=7.53 PRIMER_INTERNAL_110_SELF_END_TH=0.00 PRIMER_INTERNAL_110_HAIRPIN_TH=43.02 PRIMER_INTERNAL_111_PENALTY=7.015932 PRIMER_INTERNAL_111_SEQUENCE=TCCCGACCCAAATCGATAGCTACGATG PRIMER_INTERNAL_111=77,27 PRIMER_INTERNAL_111_TM=60.016 PRIMER_INTERNAL_111_GC_PERCENT=51.852 PRIMER_INTERNAL_111_SELF_ANY_TH=5.23 PRIMER_INTERNAL_111_SELF_END_TH=0.00 PRIMER_INTERNAL_111_HAIRPIN_TH=43.02 PRIMER_INTERNAL_112_PENALTY=7.051167 PRIMER_INTERNAL_112_SEQUENCE=TTAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_112=61,25 PRIMER_INTERNAL_112_TM=62.051 PRIMER_INTERNAL_112_GC_PERCENT=60.000 PRIMER_INTERNAL_112_SELF_ANY_TH=5.61 PRIMER_INTERNAL_112_SELF_END_TH=2.93 PRIMER_INTERNAL_112_HAIRPIN_TH=41.60 PRIMER_INTERNAL_113_PENALTY=7.056335 PRIMER_INTERNAL_113_SEQUENCE=GATCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_113=75,26 PRIMER_INTERNAL_113_TM=58.944 PRIMER_INTERNAL_113_GC_PERCENT=53.846 PRIMER_INTERNAL_113_SELF_ANY_TH=5.17 PRIMER_INTERNAL_113_SELF_END_TH=5.17 PRIMER_INTERNAL_113_HAIRPIN_TH=41.28 PRIMER_INTERNAL_114_PENALTY=7.056335 PRIMER_INTERNAL_114_SEQUENCE=CGATCCCGACCCAAATCGATAGCTAC PRIMER_INTERNAL_114=74,26 PRIMER_INTERNAL_114_TM=58.944 PRIMER_INTERNAL_114_GC_PERCENT=53.846 PRIMER_INTERNAL_114_SELF_ANY_TH=0.00 PRIMER_INTERNAL_114_SELF_END_TH=0.00 PRIMER_INTERNAL_114_HAIRPIN_TH=41.28 PRIMER_INTERNAL_115_PENALTY=7.066919 PRIMER_INTERNAL_115_SEQUENCE=CACACACACACAGCACACAGGTATT PRIMER_INTERNAL_115=38,25 PRIMER_INTERNAL_115_TM=57.933 PRIMER_INTERNAL_115_GC_PERCENT=48.000 PRIMER_INTERNAL_115_SELF_ANY_TH=0.00 PRIMER_INTERNAL_115_SELF_END_TH=0.00 PRIMER_INTERNAL_115_HAIRPIN_TH=0.00 PRIMER_INTERNAL_116_PENALTY=7.070364 PRIMER_INTERNAL_116_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCC PRIMER_INTERNAL_116=56,25 PRIMER_INTERNAL_116_TM=57.930 PRIMER_INTERNAL_116_GC_PERCENT=52.000 PRIMER_INTERNAL_116_SELF_ANY_TH=4.31 PRIMER_INTERNAL_116_SELF_END_TH=0.37 PRIMER_INTERNAL_116_HAIRPIN_TH=0.00 PRIMER_INTERNAL_117_PENALTY=7.079469 PRIMER_INTERNAL_117_SEQUENCE=GCACACAGGTATTAGTGGGCCATTC PRIMER_INTERNAL_117=50,25 PRIMER_INTERNAL_117_TM=57.921 PRIMER_INTERNAL_117_GC_PERCENT=52.000 PRIMER_INTERNAL_117_SELF_ANY_TH=2.67 PRIMER_INTERNAL_117_SELF_END_TH=0.00 PRIMER_INTERNAL_117_HAIRPIN_TH=28.81 PRIMER_INTERNAL_118_PENALTY=7.094172 PRIMER_INTERNAL_118_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCCC PRIMER_INTERNAL_118=54,27 PRIMER_INTERNAL_118_TM=59.906 PRIMER_INTERNAL_118_GC_PERCENT=51.852 PRIMER_INTERNAL_118_SELF_ANY_TH=4.31 PRIMER_INTERNAL_118_SELF_END_TH=0.37 PRIMER_INTERNAL_118_HAIRPIN_TH=0.00 PRIMER_INTERNAL_119_PENALTY=7.124042 PRIMER_INTERNAL_119_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_119=71,26 PRIMER_INTERNAL_119_TM=58.876 PRIMER_INTERNAL_119_GC_PERCENT=50.000 PRIMER_INTERNAL_119_SELF_ANY_TH=0.00 PRIMER_INTERNAL_119_SELF_END_TH=0.00 PRIMER_INTERNAL_119_HAIRPIN_TH=41.60 PRIMER_INTERNAL_120_PENALTY=7.139284 PRIMER_INTERNAL_120_SEQUENCE=CACACACACACACAGCACACAGGTATT PRIMER_INTERNAL_120=36,27 PRIMER_INTERNAL_120_TM=59.861 PRIMER_INTERNAL_120_GC_PERCENT=48.148 PRIMER_INTERNAL_120_SELF_ANY_TH=0.00 PRIMER_INTERNAL_120_SELF_END_TH=0.00 PRIMER_INTERNAL_120_HAIRPIN_TH=0.00 PRIMER_INTERNAL_121_PENALTY=7.145199 PRIMER_INTERNAL_121_SEQUENCE=GCCATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_121=68,26 PRIMER_INTERNAL_121_TM=61.145 PRIMER_INTERNAL_121_GC_PERCENT=53.846 PRIMER_INTERNAL_121_SELF_ANY_TH=0.00 PRIMER_INTERNAL_121_SELF_END_TH=0.00 PRIMER_INTERNAL_121_HAIRPIN_TH=41.60 PRIMER_INTERNAL_122_PENALTY=7.145368 PRIMER_INTERNAL_122_SEQUENCE=AGCACACAGGTATTAGTGGGCCATTC PRIMER_INTERNAL_122=49,26 PRIMER_INTERNAL_122_TM=58.855 PRIMER_INTERNAL_122_GC_PERCENT=50.000 PRIMER_INTERNAL_122_SELF_ANY_TH=8.63 PRIMER_INTERNAL_122_SELF_END_TH=0.00 PRIMER_INTERNAL_122_HAIRPIN_TH=32.68 PRIMER_INTERNAL_123_PENALTY=7.171756 PRIMER_INTERNAL_123_SEQUENCE=ACAGCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_123=47,27 PRIMER_INTERNAL_123_TM=60.172 PRIMER_INTERNAL_123_GC_PERCENT=48.148 PRIMER_INTERNAL_123_SELF_ANY_TH=8.63 PRIMER_INTERNAL_123_SELF_END_TH=0.00 PRIMER_INTERNAL_123_HAIRPIN_TH=32.68 PRIMER_INTERNAL_124_PENALTY=7.192525 PRIMER_INTERNAL_124_SEQUENCE=GATCCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_124=75,27 PRIMER_INTERNAL_124_TM=59.807 PRIMER_INTERNAL_124_GC_PERCENT=51.852 PRIMER_INTERNAL_124_SELF_ANY_TH=12.68 PRIMER_INTERNAL_124_SELF_END_TH=12.68 PRIMER_INTERNAL_124_HAIRPIN_TH=41.28 PRIMER_INTERNAL_125_PENALTY=7.192525 PRIMER_INTERNAL_125_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTAC PRIMER_INTERNAL_125=73,27 PRIMER_INTERNAL_125_TM=59.807 PRIMER_INTERNAL_125_GC_PERCENT=51.852 PRIMER_INTERNAL_125_SELF_ANY_TH=0.30 PRIMER_INTERNAL_125_SELF_END_TH=0.00 PRIMER_INTERNAL_125_HAIRPIN_TH=41.28 PRIMER_INTERNAL_126_PENALTY=7.193259 PRIMER_INTERNAL_126_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCC PRIMER_INTERNAL_126=55,26 PRIMER_INTERNAL_126_TM=58.807 PRIMER_INTERNAL_126_GC_PERCENT=53.846 PRIMER_INTERNAL_126_SELF_ANY_TH=4.31 PRIMER_INTERNAL_126_SELF_END_TH=0.37 PRIMER_INTERNAL_126_HAIRPIN_TH=0.00 PRIMER_INTERNAL_127_PENALTY=7.239217 PRIMER_INTERNAL_127_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGA PRIMER_INTERNAL_127=52,25 PRIMER_INTERNAL_127_TM=57.761 PRIMER_INTERNAL_127_GC_PERCENT=48.000 PRIMER_INTERNAL_127_SELF_ANY_TH=0.00 PRIMER_INTERNAL_127_SELF_END_TH=0.00 PRIMER_INTERNAL_127_HAIRPIN_TH=0.00 PRIMER_INTERNAL_128_PENALTY=7.243997 PRIMER_INTERNAL_128_SEQUENCE=TGGGCCATTCGATCCCGACCCAAAT PRIMER_INTERNAL_128=65,25 PRIMER_INTERNAL_128_TM=62.244 PRIMER_INTERNAL_128_GC_PERCENT=56.000 PRIMER_INTERNAL_128_SELF_ANY_TH=8.58 PRIMER_INTERNAL_128_SELF_END_TH=0.00 PRIMER_INTERNAL_128_HAIRPIN_TH=41.29 PRIMER_INTERNAL_129_PENALTY=7.246964 PRIMER_INTERNAL_129_SEQUENCE=ACACACAGCACACAGGTATTAGTGGG PRIMER_INTERNAL_129=43,26 PRIMER_INTERNAL_129_TM=58.753 PRIMER_INTERNAL_129_GC_PERCENT=50.000 PRIMER_INTERNAL_129_SELF_ANY_TH=0.00 PRIMER_INTERNAL_129_SELF_END_TH=0.00 PRIMER_INTERNAL_129_HAIRPIN_TH=0.00 PRIMER_INTERNAL_130_PENALTY=7.250024 PRIMER_INTERNAL_130_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_130=71,27 PRIMER_INTERNAL_130_TM=59.750 PRIMER_INTERNAL_130_GC_PERCENT=48.148 PRIMER_INTERNAL_130_SELF_ANY_TH=0.00 PRIMER_INTERNAL_130_SELF_END_TH=0.00 PRIMER_INTERNAL_130_HAIRPIN_TH=41.60 PRIMER_INTERNAL_131_PENALTY=7.293083 PRIMER_INTERNAL_131_SEQUENCE=CACACAGGTATTAGTGGGCCATTCG PRIMER_INTERNAL_131=51,25 PRIMER_INTERNAL_131_TM=57.707 PRIMER_INTERNAL_131_GC_PERCENT=52.000 PRIMER_INTERNAL_131_SELF_ANY_TH=0.00 PRIMER_INTERNAL_131_SELF_END_TH=0.00 PRIMER_INTERNAL_131_HAIRPIN_TH=0.00 PRIMER_INTERNAL_132_PENALTY=7.342607 PRIMER_INTERNAL_132_SEQUENCE=CATTCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_132=70,27 PRIMER_INTERNAL_132_TM=59.657 PRIMER_INTERNAL_132_GC_PERCENT=51.852 PRIMER_INTERNAL_132_SELF_ANY_TH=0.00 PRIMER_INTERNAL_132_SELF_END_TH=0.00 PRIMER_INTERNAL_132_HAIRPIN_TH=41.60 PRIMER_INTERNAL_133_PENALTY=7.350773 PRIMER_INTERNAL_133_SEQUENCE=CAGCACACAGGTATTAGTGGGCCATTC PRIMER_INTERNAL_133=48,27 PRIMER_INTERNAL_133_TM=59.649 PRIMER_INTERNAL_133_GC_PERCENT=51.852 PRIMER_INTERNAL_133_SELF_ANY_TH=8.63 PRIMER_INTERNAL_133_SELF_END_TH=0.00 PRIMER_INTERNAL_133_HAIRPIN_TH=32.68 PRIMER_INTERNAL_134_PENALTY=7.354919 PRIMER_INTERNAL_134_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGA PRIMER_INTERNAL_134=51,26 PRIMER_INTERNAL_134_TM=58.645 PRIMER_INTERNAL_134_GC_PERCENT=50.000 PRIMER_INTERNAL_134_SELF_ANY_TH=0.00 PRIMER_INTERNAL_134_SELF_END_TH=0.00 PRIMER_INTERNAL_134_HAIRPIN_TH=0.00 PRIMER_INTERNAL_135_PENALTY=7.401440 PRIMER_INTERNAL_135_SEQUENCE=CCATTCGATCCCGACCCAAATCGATA PRIMER_INTERNAL_135=69,26 PRIMER_INTERNAL_135_TM=58.599 PRIMER_INTERNAL_135_GC_PERCENT=50.000 PRIMER_INTERNAL_135_SELF_ANY_TH=0.00 PRIMER_INTERNAL_135_SELF_END_TH=0.00 PRIMER_INTERNAL_135_HAIRPIN_TH=41.60 PRIMER_INTERNAL_136_PENALTY=7.408271 PRIMER_INTERNAL_136_SEQUENCE=CACACAGCACACAGGTATTAGTGGG PRIMER_INTERNAL_136=44,25 PRIMER_INTERNAL_136_TM=57.592 PRIMER_INTERNAL_136_GC_PERCENT=52.000 PRIMER_INTERNAL_136_SELF_ANY_TH=0.00 PRIMER_INTERNAL_136_SELF_END_TH=0.00 PRIMER_INTERNAL_136_HAIRPIN_TH=0.00 PRIMER_INTERNAL_137_PENALTY=7.451841 PRIMER_INTERNAL_137_SEQUENCE=CACACACAGCACACAGGTATTAGTGGG PRIMER_INTERNAL_137=42,27 PRIMER_INTERNAL_137_TM=59.548 PRIMER_INTERNAL_137_GC_PERCENT=51.852 PRIMER_INTERNAL_137_SELF_ANY_TH=0.00 PRIMER_INTERNAL_137_SELF_END_TH=0.00 PRIMER_INTERNAL_137_HAIRPIN_TH=0.00 PRIMER_INTERNAL_138_PENALTY=7.534114 PRIMER_INTERNAL_138_SEQUENCE=CCGACCCAAATCGATAGCTACGATG PRIMER_INTERNAL_138=79,25 PRIMER_INTERNAL_138_TM=57.466 PRIMER_INTERNAL_138_GC_PERCENT=52.000 PRIMER_INTERNAL_138_SELF_ANY_TH=5.23 PRIMER_INTERNAL_138_SELF_END_TH=0.00 PRIMER_INTERNAL_138_HAIRPIN_TH=43.02 PRIMER_INTERNAL_139_PENALTY=7.537719 PRIMER_INTERNAL_139_SEQUENCE=CGACCCAAATCGATAGCTACGATGACG PRIMER_INTERNAL_139=80,27 PRIMER_INTERNAL_139_TM=59.462 PRIMER_INTERNAL_139_GC_PERCENT=51.852 PRIMER_INTERNAL_139_SELF_ANY_TH=6.52 PRIMER_INTERNAL_139_SELF_END_TH=4.23 PRIMER_INTERNAL_139_HAIRPIN_TH=43.02 PRIMER_INTERNAL_140_PENALTY=7.602591 PRIMER_INTERNAL_140_SEQUENCE=CCGACCCAAATCGATAGCTACGATGA PRIMER_INTERNAL_140=79,26 PRIMER_INTERNAL_140_TM=58.397 PRIMER_INTERNAL_140_GC_PERCENT=50.000 PRIMER_INTERNAL_140_SELF_ANY_TH=7.53 PRIMER_INTERNAL_140_SELF_END_TH=0.00 PRIMER_INTERNAL_140_HAIRPIN_TH=43.02 PRIMER_INTERNAL_141_PENALTY=7.672898 PRIMER_INTERNAL_141_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGAT PRIMER_INTERNAL_141=76,27 PRIMER_INTERNAL_141_TM=59.327 PRIMER_INTERNAL_141_GC_PERCENT=48.148 PRIMER_INTERNAL_141_SELF_ANY_TH=14.48 PRIMER_INTERNAL_141_SELF_END_TH=14.48 PRIMER_INTERNAL_141_HAIRPIN_TH=46.54 PRIMER_INTERNAL_142_PENALTY=7.693260 PRIMER_INTERNAL_142_SEQUENCE=GCCATTCGATCCCGACCCAAATCGATA PRIMER_INTERNAL_142=68,27 PRIMER_INTERNAL_142_TM=60.693 PRIMER_INTERNAL_142_GC_PERCENT=51.852 PRIMER_INTERNAL_142_SELF_ANY_TH=0.00 PRIMER_INTERNAL_142_SELF_END_TH=0.00 PRIMER_INTERNAL_142_HAIRPIN_TH=41.60 PRIMER_INTERNAL_143_PENALTY=7.693499 PRIMER_INTERNAL_143_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGACC PRIMER_INTERNAL_143=58,27 PRIMER_INTERNAL_143_TM=60.693 PRIMER_INTERNAL_143_GC_PERCENT=55.556 PRIMER_INTERNAL_143_SELF_ANY_TH=5.61 PRIMER_INTERNAL_143_SELF_END_TH=0.00 PRIMER_INTERNAL_143_HAIRPIN_TH=41.60 PRIMER_INTERNAL_144_PENALTY=7.693499 PRIMER_INTERNAL_144_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_144=57,27 PRIMER_INTERNAL_144_TM=60.693 PRIMER_INTERNAL_144_GC_PERCENT=55.556 PRIMER_INTERNAL_144_SELF_ANY_TH=3.57 PRIMER_INTERNAL_144_SELF_END_TH=3.02 PRIMER_INTERNAL_144_HAIRPIN_TH=41.60 PRIMER_INTERNAL_145_PENALTY=7.694218 PRIMER_INTERNAL_145_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_145=55,27 PRIMER_INTERNAL_145_TM=60.694 PRIMER_INTERNAL_145_GC_PERCENT=55.556 PRIMER_INTERNAL_145_SELF_ANY_TH=4.31 PRIMER_INTERNAL_145_SELF_END_TH=0.00 PRIMER_INTERNAL_145_HAIRPIN_TH=37.07 PRIMER_INTERNAL_146_PENALTY=7.696389 PRIMER_INTERNAL_146_SEQUENCE=ACACACACACACACAGCACACAGGTAT PRIMER_INTERNAL_146=35,27 PRIMER_INTERNAL_146_TM=60.696 PRIMER_INTERNAL_146_GC_PERCENT=48.148 PRIMER_INTERNAL_146_SELF_ANY_TH=0.00 PRIMER_INTERNAL_146_SELF_END_TH=0.00 PRIMER_INTERNAL_146_HAIRPIN_TH=0.00 PRIMER_INTERNAL_147_PENALTY=7.699801 PRIMER_INTERNAL_147_SEQUENCE=CACAGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_147=46,27 PRIMER_INTERNAL_147_TM=60.700 PRIMER_INTERNAL_147_GC_PERCENT=51.852 PRIMER_INTERNAL_147_SELF_ANY_TH=8.63 PRIMER_INTERNAL_147_SELF_END_TH=0.00 PRIMER_INTERNAL_147_HAIRPIN_TH=32.68 PRIMER_INTERNAL_148_PENALTY=7.723400 PRIMER_INTERNAL_148_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCTA PRIMER_INTERNAL_148=72,27 PRIMER_INTERNAL_148_TM=59.277 PRIMER_INTERNAL_148_GC_PERCENT=48.148 PRIMER_INTERNAL_148_SELF_ANY_TH=0.00 PRIMER_INTERNAL_148_SELF_END_TH=0.00 PRIMER_INTERNAL_148_HAIRPIN_TH=41.60 PRIMER_INTERNAL_149_PENALTY=7.748120 PRIMER_INTERNAL_149_SEQUENCE=GCACACAGGTATTAGTGGGCCATTCGA PRIMER_INTERNAL_149=50,27 PRIMER_INTERNAL_149_TM=60.748 PRIMER_INTERNAL_149_GC_PERCENT=51.852 PRIMER_INTERNAL_149_SELF_ANY_TH=2.67 PRIMER_INTERNAL_149_SELF_END_TH=0.00 PRIMER_INTERNAL_149_HAIRPIN_TH=28.81 PRIMER_INTERNAL_150_PENALTY=7.749187 PRIMER_INTERNAL_150_SEQUENCE=AGCACACAGGTATTAGTGGGCCATTCG PRIMER_INTERNAL_150=49,27 PRIMER_INTERNAL_150_TM=60.749 PRIMER_INTERNAL_150_GC_PERCENT=51.852 PRIMER_INTERNAL_150_SELF_ANY_TH=8.63 PRIMER_INTERNAL_150_SELF_END_TH=0.00 PRIMER_INTERNAL_150_HAIRPIN_TH=32.68 PRIMER_INTERNAL_151_PENALTY=7.753661 PRIMER_INTERNAL_151_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCC PRIMER_INTERNAL_151=54,26 PRIMER_INTERNAL_151_TM=58.246 PRIMER_INTERNAL_151_GC_PERCENT=50.000 PRIMER_INTERNAL_151_SELF_ANY_TH=2.01 PRIMER_INTERNAL_151_SELF_END_TH=1.68 PRIMER_INTERNAL_151_HAIRPIN_TH=0.00 PRIMER_INTERNAL_152_PENALTY=7.785822 PRIMER_INTERNAL_152_SEQUENCE=CGATCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_152=74,27 PRIMER_INTERNAL_152_TM=60.786 PRIMER_INTERNAL_152_GC_PERCENT=55.556 PRIMER_INTERNAL_152_SELF_ANY_TH=5.17 PRIMER_INTERNAL_152_SELF_END_TH=5.17 PRIMER_INTERNAL_152_HAIRPIN_TH=41.28 PRIMER_INTERNAL_153_PENALTY=7.802259 PRIMER_INTERNAL_153_SEQUENCE=CCGACCCAAATCGATAGCTACGATGAC PRIMER_INTERNAL_153=79,27 PRIMER_INTERNAL_153_TM=59.198 PRIMER_INTERNAL_153_GC_PERCENT=51.852 PRIMER_INTERNAL_153_SELF_ANY_TH=5.43 PRIMER_INTERNAL_153_SELF_END_TH=0.00 PRIMER_INTERNAL_153_HAIRPIN_TH=43.02 PRIMER_INTERNAL_154_PENALTY=7.812760 PRIMER_INTERNAL_154_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_154=56,27 PRIMER_INTERNAL_154_TM=60.813 PRIMER_INTERNAL_154_GC_PERCENT=51.852 PRIMER_INTERNAL_154_SELF_ANY_TH=4.31 PRIMER_INTERNAL_154_SELF_END_TH=0.00 PRIMER_INTERNAL_154_HAIRPIN_TH=41.96 PRIMER_INTERNAL_155_PENALTY=7.812835 PRIMER_INTERNAL_155_SEQUENCE=CCATTCGATCCCGACCCAAATCGATAG PRIMER_INTERNAL_155=69,27 PRIMER_INTERNAL_155_TM=59.187 PRIMER_INTERNAL_155_GC_PERCENT=51.852 PRIMER_INTERNAL_155_SELF_ANY_TH=0.00 PRIMER_INTERNAL_155_SELF_END_TH=0.00 PRIMER_INTERNAL_155_HAIRPIN_TH=41.60 PRIMER_INTERNAL_156_PENALTY=7.853399 PRIMER_INTERNAL_156_SEQUENCE=ACACACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_156=43,27 PRIMER_INTERNAL_156_TM=60.853 PRIMER_INTERNAL_156_GC_PERCENT=51.852 PRIMER_INTERNAL_156_SELF_ANY_TH=5.21 PRIMER_INTERNAL_156_SELF_END_TH=5.21 PRIMER_INTERNAL_156_HAIRPIN_TH=33.51 PRIMER_INTERNAL_157_PENALTY=7.928092 PRIMER_INTERNAL_157_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATCC PRIMER_INTERNAL_157=53,27 PRIMER_INTERNAL_157_TM=59.072 PRIMER_INTERNAL_157_GC_PERCENT=51.852 PRIMER_INTERNAL_157_SELF_ANY_TH=2.01 PRIMER_INTERNAL_157_SELF_END_TH=1.68 PRIMER_INTERNAL_157_HAIRPIN_TH=0.00 PRIMER_INTERNAL_158_PENALTY=7.938713 PRIMER_INTERNAL_158_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCC PRIMER_INTERNAL_158=55,25 PRIMER_INTERNAL_158_TM=57.061 PRIMER_INTERNAL_158_GC_PERCENT=52.000 PRIMER_INTERNAL_158_SELF_ANY_TH=2.01 PRIMER_INTERNAL_158_SELF_END_TH=1.68 PRIMER_INTERNAL_158_HAIRPIN_TH=0.00 PRIMER_INTERNAL_159_PENALTY=7.949148 PRIMER_INTERNAL_159_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAA PRIMER_INTERNAL_159=64,25 PRIMER_INTERNAL_159_TM=62.949 PRIMER_INTERNAL_159_GC_PERCENT=60.000 PRIMER_INTERNAL_159_SELF_ANY_TH=8.58 PRIMER_INTERNAL_159_SELF_END_TH=0.00 PRIMER_INTERNAL_159_HAIRPIN_TH=43.79 PRIMER_INTERNAL_160_PENALTY=7.979746 PRIMER_INTERNAL_160_SEQUENCE=ACACACACAGCACACAGGTATTAGTGG PRIMER_INTERNAL_160=41,27 PRIMER_INTERNAL_160_TM=59.020 PRIMER_INTERNAL_160_GC_PERCENT=48.148 PRIMER_INTERNAL_160_SELF_ANY_TH=0.00 PRIMER_INTERNAL_160_SELF_END_TH=0.00 PRIMER_INTERNAL_160_HAIRPIN_TH=0.00 PRIMER_INTERNAL_161_PENALTY=7.992102 PRIMER_INTERNAL_161_SEQUENCE=TAGTGGGCCATTCGATCCCGACCCA PRIMER_INTERNAL_161=62,25 PRIMER_INTERNAL_161_TM=62.992 PRIMER_INTERNAL_161_GC_PERCENT=60.000 PRIMER_INTERNAL_161_SELF_ANY_TH=8.58 PRIMER_INTERNAL_161_SELF_END_TH=6.40 PRIMER_INTERNAL_161_HAIRPIN_TH=42.67 PRIMER_INTERNAL_162_PENALTY=7.993148 PRIMER_INTERNAL_162_SEQUENCE=ACACACAGCACACAGGTATTAGTGG PRIMER_INTERNAL_162=43,25 PRIMER_INTERNAL_162_TM=57.007 PRIMER_INTERNAL_162_GC_PERCENT=48.000 PRIMER_INTERNAL_162_SELF_ANY_TH=0.00 PRIMER_INTERNAL_162_SELF_END_TH=0.00 PRIMER_INTERNAL_162_HAIRPIN_TH=0.00 PRIMER_INTERNAL_163_PENALTY=8.038590 PRIMER_INTERNAL_163_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_163=60,26 PRIMER_INTERNAL_163_TM=62.039 PRIMER_INTERNAL_163_GC_PERCENT=57.692 PRIMER_INTERNAL_163_SELF_ANY_TH=5.61 PRIMER_INTERNAL_163_SELF_END_TH=2.93 PRIMER_INTERNAL_163_HAIRPIN_TH=41.60 PRIMER_INTERNAL_164_PENALTY=8.084641 PRIMER_INTERNAL_164_SEQUENCE=CACACACAGCACACAGGTATTAGTGG PRIMER_INTERNAL_164=42,26 PRIMER_INTERNAL_164_TM=57.915 PRIMER_INTERNAL_164_GC_PERCENT=50.000 PRIMER_INTERNAL_164_SELF_ANY_TH=0.00 PRIMER_INTERNAL_164_SELF_END_TH=0.00 PRIMER_INTERNAL_164_HAIRPIN_TH=0.00 PRIMER_INTERNAL_165_PENALTY=8.098597 PRIMER_INTERNAL_165_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGAT PRIMER_INTERNAL_165=52,26 PRIMER_INTERNAL_165_TM=57.901 PRIMER_INTERNAL_165_GC_PERCENT=46.154 PRIMER_INTERNAL_165_SELF_ANY_TH=0.00 PRIMER_INTERNAL_165_SELF_END_TH=0.00 PRIMER_INTERNAL_165_HAIRPIN_TH=0.00 PRIMER_INTERNAL_166_PENALTY=8.106799 PRIMER_INTERNAL_166_SEQUENCE=TCAGTAGACATGACACTGACGATGCA PRIMER_INTERNAL_166=5,26 PRIMER_INTERNAL_166_TM=57.893 PRIMER_INTERNAL_166_GC_PERCENT=46.154 PRIMER_INTERNAL_166_SELF_ANY_TH=16.57 PRIMER_INTERNAL_166_SELF_END_TH=0.76 PRIMER_INTERNAL_166_HAIRPIN_TH=0.00 PRIMER_INTERNAL_167_PENALTY=8.251191 PRIMER_INTERNAL_167_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGAT PRIMER_INTERNAL_167=51,27 PRIMER_INTERNAL_167_TM=58.749 PRIMER_INTERNAL_167_GC_PERCENT=48.148 PRIMER_INTERNAL_167_SELF_ANY_TH=0.00 PRIMER_INTERNAL_167_SELF_END_TH=0.00 PRIMER_INTERNAL_167_HAIRPIN_TH=0.00 PRIMER_INTERNAL_168_PENALTY=8.275978 PRIMER_INTERNAL_168_SEQUENCE=GTCAGTAGACATGACACTGACGATGCA PRIMER_INTERNAL_168=4,27 PRIMER_INTERNAL_168_TM=58.724 PRIMER_INTERNAL_168_GC_PERCENT=48.148 PRIMER_INTERNAL_168_SELF_ANY_TH=22.48 PRIMER_INTERNAL_168_SELF_END_TH=5.75 PRIMER_INTERNAL_168_HAIRPIN_TH=41.09 PRIMER_INTERNAL_169_PENALTY=8.300145 PRIMER_INTERNAL_169_SEQUENCE=ACACACACACACAGCACACAGGTATTA PRIMER_INTERNAL_169=37,27 PRIMER_INTERNAL_169_TM=58.700 PRIMER_INTERNAL_169_GC_PERCENT=44.444 PRIMER_INTERNAL_169_SELF_ANY_TH=0.00 PRIMER_INTERNAL_169_SELF_END_TH=0.00 PRIMER_INTERNAL_169_HAIRPIN_TH=0.00 PRIMER_INTERNAL_170_PENALTY=8.330872 PRIMER_INTERNAL_170_SEQUENCE=AGTAGACATGACACTGACGATGCAGA PRIMER_INTERNAL_170=7,26 PRIMER_INTERNAL_170_TM=57.669 PRIMER_INTERNAL_170_GC_PERCENT=46.154 PRIMER_INTERNAL_170_SELF_ANY_TH=7.76 PRIMER_INTERNAL_170_SELF_END_TH=0.00 PRIMER_INTERNAL_170_HAIRPIN_TH=0.00 PRIMER_INTERNAL_171_PENALTY=8.369366 PRIMER_INTERNAL_171_SEQUENCE=CACACAGCACACAGGTATTAGTGGGCC PRIMER_INTERNAL_171=44,27 PRIMER_INTERNAL_171_TM=61.369 PRIMER_INTERNAL_171_GC_PERCENT=55.556 PRIMER_INTERNAL_171_SELF_ANY_TH=8.63 PRIMER_INTERNAL_171_SELF_END_TH=0.00 PRIMER_INTERNAL_171_HAIRPIN_TH=32.68 PRIMER_INTERNAL_172_PENALTY=8.376203 PRIMER_INTERNAL_172_SEQUENCE=GTAGACATGACACTGACGATGCAGAC PRIMER_INTERNAL_172=8,26 PRIMER_INTERNAL_172_TM=57.624 PRIMER_INTERNAL_172_GC_PERCENT=50.000 PRIMER_INTERNAL_172_SELF_ANY_TH=1.44 PRIMER_INTERNAL_172_SELF_END_TH=0.00 PRIMER_INTERNAL_172_HAIRPIN_TH=0.00 PRIMER_INTERNAL_173_PENALTY=8.376203 PRIMER_INTERNAL_173_SEQUENCE=GTCAGTAGACATGACACTGACGATGC PRIMER_INTERNAL_173=4,26 PRIMER_INTERNAL_173_TM=57.624 PRIMER_INTERNAL_173_GC_PERCENT=50.000 PRIMER_INTERNAL_173_SELF_ANY_TH=22.48 PRIMER_INTERNAL_173_SELF_END_TH=10.90 PRIMER_INTERNAL_173_HAIRPIN_TH=41.09 PRIMER_INTERNAL_174_PENALTY=8.379879 PRIMER_INTERNAL_174_SEQUENCE=CAGTAGACATGACACTGACGATGCAG PRIMER_INTERNAL_174=6,26 PRIMER_INTERNAL_174_TM=57.620 PRIMER_INTERNAL_174_GC_PERCENT=50.000 PRIMER_INTERNAL_174_SELF_ANY_TH=12.05 PRIMER_INTERNAL_174_SELF_END_TH=0.00 PRIMER_INTERNAL_174_HAIRPIN_TH=0.00 PRIMER_INTERNAL_175_PENALTY=8.418488 PRIMER_INTERNAL_175_SEQUENCE=CACACACACACAGCACACAGGTATTA PRIMER_INTERNAL_175=38,26 PRIMER_INTERNAL_175_TM=57.582 PRIMER_INTERNAL_175_GC_PERCENT=46.154 PRIMER_INTERNAL_175_SELF_ANY_TH=0.00 PRIMER_INTERNAL_175_SELF_END_TH=0.00 PRIMER_INTERNAL_175_HAIRPIN_TH=0.00 PRIMER_INTERNAL_176_PENALTY=8.423914 PRIMER_INTERNAL_176_SEQUENCE=GACCCAAATCGATAGCTACGATGACG PRIMER_INTERNAL_176=81,26 PRIMER_INTERNAL_176_TM=57.576 PRIMER_INTERNAL_176_GC_PERCENT=50.000 PRIMER_INTERNAL_176_SELF_ANY_TH=1.59 PRIMER_INTERNAL_176_SELF_END_TH=0.00 PRIMER_INTERNAL_176_HAIRPIN_TH=43.02 PRIMER_INTERNAL_177_PENALTY=8.423914 PRIMER_INTERNAL_177_SEQUENCE=CGACCCAAATCGATAGCTACGATGAC PRIMER_INTERNAL_177=80,26 PRIMER_INTERNAL_177_TM=57.576 PRIMER_INTERNAL_177_GC_PERCENT=50.000 PRIMER_INTERNAL_177_SELF_ANY_TH=2.38 PRIMER_INTERNAL_177_SELF_END_TH=0.00 PRIMER_INTERNAL_177_HAIRPIN_TH=43.02 PRIMER_INTERNAL_178_PENALTY=8.452836 PRIMER_INTERNAL_178_SEQUENCE=CATTCGATCCCGACCCAAATCGATAG PRIMER_INTERNAL_178=70,26 PRIMER_INTERNAL_178_TM=57.547 PRIMER_INTERNAL_178_GC_PERCENT=50.000 PRIMER_INTERNAL_178_SELF_ANY_TH=0.00 PRIMER_INTERNAL_178_SELF_END_TH=0.00 PRIMER_INTERNAL_178_HAIRPIN_TH=41.60 PRIMER_INTERNAL_179_PENALTY=8.463323 PRIMER_INTERNAL_179_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGATC PRIMER_INTERNAL_179=52,27 PRIMER_INTERNAL_179_TM=58.537 PRIMER_INTERNAL_179_GC_PERCENT=48.148 PRIMER_INTERNAL_179_SELF_ANY_TH=0.00 PRIMER_INTERNAL_179_SELF_END_TH=0.00 PRIMER_INTERNAL_179_HAIRPIN_TH=0.00 PRIMER_INTERNAL_180_PENALTY=8.486477 PRIMER_INTERNAL_180_SEQUENCE=CAGTAGACATGACACTGACGATGCAGA PRIMER_INTERNAL_180=6,27 PRIMER_INTERNAL_180_TM=58.514 PRIMER_INTERNAL_180_GC_PERCENT=48.148 PRIMER_INTERNAL_180_SELF_ANY_TH=12.05 PRIMER_INTERNAL_180_SELF_END_TH=0.00 PRIMER_INTERNAL_180_HAIRPIN_TH=0.00 PRIMER_INTERNAL_181_PENALTY=8.486477 PRIMER_INTERNAL_181_SEQUENCE=TCAGTAGACATGACACTGACGATGCAG PRIMER_INTERNAL_181=5,27 PRIMER_INTERNAL_181_TM=58.514 PRIMER_INTERNAL_181_GC_PERCENT=48.148 PRIMER_INTERNAL_181_SELF_ANY_TH=16.57 PRIMER_INTERNAL_181_SELF_END_TH=0.00 PRIMER_INTERNAL_181_HAIRPIN_TH=0.00 PRIMER_INTERNAL_182_PENALTY=8.487256 PRIMER_INTERNAL_182_SEQUENCE=AGTAGACATGACACTGACGATGCAGAC PRIMER_INTERNAL_182=7,27 PRIMER_INTERNAL_182_TM=58.513 PRIMER_INTERNAL_182_GC_PERCENT=48.148 PRIMER_INTERNAL_182_SELF_ANY_TH=1.44 PRIMER_INTERNAL_182_SELF_END_TH=0.00 PRIMER_INTERNAL_182_HAIRPIN_TH=0.00 PRIMER_INTERNAL_183_PENALTY=8.487256 PRIMER_INTERNAL_183_SEQUENCE=AGTCAGTAGACATGACACTGACGATGC PRIMER_INTERNAL_183=3,27 PRIMER_INTERNAL_183_TM=58.513 PRIMER_INTERNAL_183_GC_PERCENT=48.148 PRIMER_INTERNAL_183_SELF_ANY_TH=25.46 PRIMER_INTERNAL_183_SELF_END_TH=14.56 PRIMER_INTERNAL_183_HAIRPIN_TH=43.89 PRIMER_INTERNAL_184_PENALTY=8.514997 PRIMER_INTERNAL_184_SEQUENCE=ACACACACACAGCACACAGGTATTAGT PRIMER_INTERNAL_184=39,27 PRIMER_INTERNAL_184_TM=58.485 PRIMER_INTERNAL_184_GC_PERCENT=44.444 PRIMER_INTERNAL_184_SELF_ANY_TH=0.00 PRIMER_INTERNAL_184_SELF_END_TH=0.00 PRIMER_INTERNAL_184_HAIRPIN_TH=0.00 PRIMER_INTERNAL_185_PENALTY=8.547914 PRIMER_INTERNAL_185_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_185=59,27 PRIMER_INTERNAL_185_TM=61.548 PRIMER_INTERNAL_185_GC_PERCENT=55.556 PRIMER_INTERNAL_185_SELF_ANY_TH=5.61 PRIMER_INTERNAL_185_SELF_END_TH=2.93 PRIMER_INTERNAL_185_HAIRPIN_TH=41.60 PRIMER_INTERNAL_186_PENALTY=8.589885 PRIMER_INTERNAL_186_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATC PRIMER_INTERNAL_186=53,26 PRIMER_INTERNAL_186_TM=57.410 PRIMER_INTERNAL_186_GC_PERCENT=50.000 PRIMER_INTERNAL_186_SELF_ANY_TH=0.00 PRIMER_INTERNAL_186_SELF_END_TH=0.00 PRIMER_INTERNAL_186_HAIRPIN_TH=0.00 PRIMER_INTERNAL_187_PENALTY=8.642220 PRIMER_INTERNAL_187_SEQUENCE=ACACACACAGCACACAGGTATTAGTG PRIMER_INTERNAL_187=41,26 PRIMER_INTERNAL_187_TM=57.358 PRIMER_INTERNAL_187_GC_PERCENT=46.154 PRIMER_INTERNAL_187_SELF_ANY_TH=0.00 PRIMER_INTERNAL_187_SELF_END_TH=0.00 PRIMER_INTERNAL_187_HAIRPIN_TH=0.00 PRIMER_INTERNAL_188_PENALTY=8.642220 PRIMER_INTERNAL_188_SEQUENCE=CACACACACAGCACACAGGTATTAGT PRIMER_INTERNAL_188=40,26 PRIMER_INTERNAL_188_TM=57.358 PRIMER_INTERNAL_188_GC_PERCENT=46.154 PRIMER_INTERNAL_188_SELF_ANY_TH=0.00 PRIMER_INTERNAL_188_SELF_END_TH=0.00 PRIMER_INTERNAL_188_HAIRPIN_TH=0.00 PRIMER_INTERNAL_189_PENALTY=8.642220 PRIMER_INTERNAL_189_SEQUENCE=ACACACACACAGCACACAGGTATTAG PRIMER_INTERNAL_189=39,26 PRIMER_INTERNAL_189_TM=57.358 PRIMER_INTERNAL_189_GC_PERCENT=46.154 PRIMER_INTERNAL_189_SELF_ANY_TH=0.00 PRIMER_INTERNAL_189_SELF_END_TH=0.00 PRIMER_INTERNAL_189_HAIRPIN_TH=0.00 PRIMER_INTERNAL_190_PENALTY=8.687221 PRIMER_INTERNAL_190_SEQUENCE=TGGGCCATTCGATCCCGACCCAAATC PRIMER_INTERNAL_190=65,26 PRIMER_INTERNAL_190_TM=62.687 PRIMER_INTERNAL_190_GC_PERCENT=57.692 PRIMER_INTERNAL_190_SELF_ANY_TH=8.58 PRIMER_INTERNAL_190_SELF_END_TH=0.00 PRIMER_INTERNAL_190_HAIRPIN_TH=41.29 PRIMER_INTERNAL_191_PENALTY=8.713667 PRIMER_INTERNAL_191_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_191=67,26 PRIMER_INTERNAL_191_TM=62.714 PRIMER_INTERNAL_191_GC_PERCENT=57.692 PRIMER_INTERNAL_191_SELF_ANY_TH=0.00 PRIMER_INTERNAL_191_SELF_END_TH=0.00 PRIMER_INTERNAL_191_HAIRPIN_TH=41.60 PRIMER_INTERNAL_192_PENALTY=8.714713 PRIMER_INTERNAL_192_SEQUENCE=ACACAGCACACAGGTATTAGTGGGCCA PRIMER_INTERNAL_192=45,27 PRIMER_INTERNAL_192_TM=61.715 PRIMER_INTERNAL_192_GC_PERCENT=51.852 PRIMER_INTERNAL_192_SELF_ANY_TH=8.63 PRIMER_INTERNAL_192_SELF_END_TH=1.50 PRIMER_INTERNAL_192_HAIRPIN_TH=32.68 PRIMER_INTERNAL_193_PENALTY=8.787348 PRIMER_INTERNAL_193_SEQUENCE=CACACACACAGCACACAGGTATTAGTG PRIMER_INTERNAL_193=40,27 PRIMER_INTERNAL_193_TM=58.213 PRIMER_INTERNAL_193_GC_PERCENT=48.148 PRIMER_INTERNAL_193_SELF_ANY_TH=0.00 PRIMER_INTERNAL_193_SELF_END_TH=0.00 PRIMER_INTERNAL_193_HAIRPIN_TH=0.00 PRIMER_INTERNAL_194_PENALTY=8.787348 PRIMER_INTERNAL_194_SEQUENCE=CACACACACACAGCACACAGGTATTAG PRIMER_INTERNAL_194=38,27 PRIMER_INTERNAL_194_TM=58.213 PRIMER_INTERNAL_194_GC_PERCENT=48.148 PRIMER_INTERNAL_194_SELF_ANY_TH=0.00 PRIMER_INTERNAL_194_SELF_END_TH=0.00 PRIMER_INTERNAL_194_HAIRPIN_TH=0.00 PRIMER_INTERNAL_195_PENALTY=8.904972 PRIMER_INTERNAL_195_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAAT PRIMER_INTERNAL_195=64,26 PRIMER_INTERNAL_195_TM=62.905 PRIMER_INTERNAL_195_GC_PERCENT=57.692 PRIMER_INTERNAL_195_SELF_ANY_TH=8.58 PRIMER_INTERNAL_195_SELF_END_TH=0.00 PRIMER_INTERNAL_195_HAIRPIN_TH=43.79 PRIMER_INTERNAL_196_PENALTY=9.679371 PRIMER_INTERNAL_196_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_196=67,27 PRIMER_INTERNAL_196_TM=62.679 PRIMER_INTERNAL_196_GC_PERCENT=55.556 PRIMER_INTERNAL_196_SELF_ANY_TH=0.00 PRIMER_INTERNAL_196_SELF_END_TH=0.00 PRIMER_INTERNAL_196_HAIRPIN_TH=41.60 = PRIMER_PRODUCT_SIZE_RANGE=75-100 P3_FILE_FLAG=1 SEQUENCE_ID=pick_primer_list_and_file_flag_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33 PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33 PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=33 PRIMER_RIGHT_NUM_RETURNED=33 PRIMER_INTERNAL_NUM_RETURNED=73 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.486439 PRIMER_RIGHT_0_PENALTY=0.486439 PRIMER_INTERNAL_0_PENALTY=0.093814 PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA PRIMER_LEFT_0=118,20 PRIMER_RIGHT_0=137,20 PRIMER_INTERNAL_0=125,20 PRIMER_LEFT_0_TM=58.514 PRIMER_RIGHT_0_TM=58.514 PRIMER_INTERNAL_0_TM=59.906 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_SELF_ANY_TH=7.82 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=36.42 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon PRIMER_LEFT_0_END_STABILITY=5.3400 PRIMER_RIGHT_0_END_STABILITY=3.0100 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=8.2325 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0760 PRIMER_LEFT_1_PENALTY=1.160593 PRIMER_RIGHT_1_PENALTY=1.160593 PRIMER_INTERNAL_1_PENALTY=0.301069 PRIMER_LEFT_1_SEQUENCE=CAAAACGCAAAGCACGCTC PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTTG PRIMER_INTERNAL_1_SEQUENCE=ACGCAAAGCACGCTCCCGAT PRIMER_LEFT_1=122,19 PRIMER_RIGHT_1=140,19 PRIMER_INTERNAL_1=126,20 PRIMER_LEFT_1_TM=59.161 PRIMER_RIGHT_1_TM=59.161 PRIMER_INTERNAL_1_TM=59.699 PRIMER_LEFT_1_GC_PERCENT=52.632 PRIMER_RIGHT_1_GC_PERCENT=52.632 PRIMER_INTERNAL_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_SELF_ANY_TH=7.82 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=37.44 PRIMER_INTERNAL_1_HAIRPIN_TH=36.42 PRIMER_LEFT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_RIGHT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_1_END_STABILITY=5.0300 PRIMER_RIGHT_1_END_STABILITY=2.4400 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=5.7937 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=8.3885 PRIMER_LEFT_2_PENALTY=1.656085 PRIMER_RIGHT_2_PENALTY=1.656085 PRIMER_INTERNAL_2_PENALTY=0.900305 PRIMER_LEFT_2_SEQUENCE=TATTCCAAAACGCAAAGCACG PRIMER_RIGHT_2_SEQUENCE=CGTGCTTTGCGTTTTGGAATA PRIMER_INTERNAL_2_SEQUENCE=AAACGCAAAGCACGCTCCCG PRIMER_LEFT_2=117,21 PRIMER_RIGHT_2=137,21 PRIMER_INTERNAL_2=124,20 PRIMER_LEFT_2_TM=58.344 PRIMER_RIGHT_2_TM=58.344 PRIMER_INTERNAL_2_TM=59.100 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_SELF_ANY_TH=1.50 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=1.50 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=41.14 PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, reverse MER46 Nonautonomous DNA transposon PRIMER_LEFT_2_END_STABILITY=5.3400 PRIMER_RIGHT_2_END_STABILITY=1.7500 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=10.3450 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=9.4271 PRIMER_LEFT_3_PENALTY=1.695359 PRIMER_RIGHT_3_PENALTY=1.695359 PRIMER_INTERNAL_3_PENALTY=0.956520 PRIMER_LEFT_3_SEQUENCE=TGACCTGATAGAAACGCCAAC PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA PRIMER_INTERNAL_3_SEQUENCE=CGCAAAGCACGCTCCCGATC PRIMER_LEFT_3=53,21 PRIMER_RIGHT_3=73,21 PRIMER_INTERNAL_3=127,20 PRIMER_LEFT_3_TM=58.305 PRIMER_RIGHT_3_TM=58.305 PRIMER_INTERNAL_3_TM=59.043 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=36.42 PRIMER_LEFT_3_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene PRIMER_RIGHT_3_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=3.7700 PRIMER_RIGHT_3_END_STABILITY=4.0200 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=6.6359 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.3924 PRIMER_LEFT_4_PENALTY=1.831685 PRIMER_RIGHT_4_PENALTY=1.831685 PRIMER_INTERNAL_4_PENALTY=1.018091 PRIMER_LEFT_4_SEQUENCE=AACGCCAACTCACTGTATGA PRIMER_RIGHT_4_SEQUENCE=TCATACAGTGAGTTGGCGTT PRIMER_INTERNAL_4_SEQUENCE=AACGCAAAGCACGCTCCCGAT PRIMER_LEFT_4=65,20 PRIMER_RIGHT_4=84,20 PRIMER_INTERNAL_4=125,21 PRIMER_LEFT_4_TM=57.168 PRIMER_RIGHT_4_TM=57.168 PRIMER_INTERNAL_4_TM=59.982 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=57.143 PRIMER_INTERNAL_4_SELF_ANY_TH=7.82 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=35.45 PRIMER_RIGHT_4_HAIRPIN_TH=31.80 PRIMER_INTERNAL_4_HAIRPIN_TH=36.42 PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_RIGHT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_LEFT_4_END_STABILITY=2.1500 PRIMER_RIGHT_4_END_STABILITY=4.8400 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=18.7112 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=25.7856 PRIMER_LEFT_5_PENALTY=1.902705 PRIMER_RIGHT_5_PENALTY=1.902705 PRIMER_INTERNAL_5_PENALTY=1.178696 PRIMER_LEFT_5_SEQUENCE=AGAAACGCCAACTCACTGTA PRIMER_RIGHT_5_SEQUENCE=TACAGTGAGTTGGCGTTTCT PRIMER_INTERNAL_5_SEQUENCE=AAACGCAAAGCACGCTCCCGA PRIMER_LEFT_5=62,20 PRIMER_RIGHT_5=81,20 PRIMER_INTERNAL_5=124,21 PRIMER_LEFT_5_TM=57.097 PRIMER_RIGHT_5_TM=57.097 PRIMER_INTERNAL_5_TM=60.179 PRIMER_LEFT_5_GC_PERCENT=45.000 PRIMER_RIGHT_5_GC_PERCENT=45.000 PRIMER_INTERNAL_5_GC_PERCENT=57.143 PRIMER_INTERNAL_5_SELF_ANY_TH=7.82 PRIMER_LEFT_5_SELF_ANY_TH=0.00 PRIMER_RIGHT_5_SELF_ANY_TH=0.00 PRIMER_INTERNAL_5_SELF_END_TH=0.00 PRIMER_LEFT_5_SELF_END_TH=0.00 PRIMER_RIGHT_5_SELF_END_TH=0.00 PRIMER_LEFT_5_HAIRPIN_TH=0.00 PRIMER_RIGHT_5_HAIRPIN_TH=0.00 PRIMER_INTERNAL_5_HAIRPIN_TH=36.42 PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_5_END_STABILITY=2.7400 PRIMER_RIGHT_5_END_STABILITY=2.5200 PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=15.5573 PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=19.2811 PRIMER_LEFT_6_PENALTY=1.902705 PRIMER_RIGHT_6_PENALTY=1.902705 PRIMER_INTERNAL_6_PENALTY=1.372876 PRIMER_LEFT_6_SEQUENCE=TAGAAACGCCAACTCACTGT PRIMER_RIGHT_6_SEQUENCE=ACAGTGAGTTGGCGTTTCTA PRIMER_INTERNAL_6_SEQUENCE=ACGCAAAGCACGCTCCCGATC PRIMER_LEFT_6=61,20 PRIMER_RIGHT_6=80,20 PRIMER_INTERNAL_6=126,21 PRIMER_LEFT_6_TM=57.097 PRIMER_RIGHT_6_TM=57.097 PRIMER_INTERNAL_6_TM=60.373 PRIMER_LEFT_6_GC_PERCENT=45.000 PRIMER_RIGHT_6_GC_PERCENT=45.000 PRIMER_INTERNAL_6_GC_PERCENT=61.905 PRIMER_INTERNAL_6_SELF_ANY_TH=7.82 PRIMER_LEFT_6_SELF_ANY_TH=0.00 PRIMER_RIGHT_6_SELF_ANY_TH=0.00 PRIMER_INTERNAL_6_SELF_END_TH=0.00 PRIMER_LEFT_6_SELF_END_TH=0.00 PRIMER_RIGHT_6_SELF_END_TH=0.00 PRIMER_LEFT_6_HAIRPIN_TH=0.00 PRIMER_RIGHT_6_HAIRPIN_TH=0.00 PRIMER_INTERNAL_6_HAIRPIN_TH=36.42 PRIMER_LEFT_6_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_6_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_6_END_STABILITY=3.5500 PRIMER_RIGHT_6_END_STABILITY=2.1000 PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=10.6833 PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=20.2263 PRIMER_LEFT_7_PENALTY=1.950877 PRIMER_RIGHT_7_PENALTY=1.950877 PRIMER_INTERNAL_7_PENALTY=1.393730 PRIMER_LEFT_7_SEQUENCE=TTCCAAAACGCAAAGCACG PRIMER_RIGHT_7_SEQUENCE=CGTGCTTTGCGTTTTGGAA PRIMER_INTERNAL_7_SEQUENCE=ACGCAAAGCACGCTCCCGA PRIMER_LEFT_7=119,19 PRIMER_RIGHT_7=137,19 PRIMER_INTERNAL_7=126,19 PRIMER_LEFT_7_TM=58.049 PRIMER_RIGHT_7_TM=58.049 PRIMER_INTERNAL_7_TM=59.606 PRIMER_LEFT_7_GC_PERCENT=47.368 PRIMER_RIGHT_7_GC_PERCENT=47.368 PRIMER_INTERNAL_7_GC_PERCENT=63.158 PRIMER_INTERNAL_7_SELF_ANY_TH=7.82 PRIMER_LEFT_7_SELF_ANY_TH=0.00 PRIMER_RIGHT_7_SELF_ANY_TH=0.00 PRIMER_INTERNAL_7_SELF_END_TH=0.00 PRIMER_LEFT_7_SELF_END_TH=0.00 PRIMER_RIGHT_7_SELF_END_TH=0.00 PRIMER_LEFT_7_HAIRPIN_TH=0.00 PRIMER_RIGHT_7_HAIRPIN_TH=0.00 PRIMER_INTERNAL_7_HAIRPIN_TH=36.42 PRIMER_LEFT_7_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_7_END_STABILITY=5.3400 PRIMER_RIGHT_7_END_STABILITY=3.5300 PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=2.1013 PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=4.8280 PRIMER_LEFT_8_PENALTY=1.972625 PRIMER_RIGHT_8_PENALTY=1.972625 PRIMER_INTERNAL_8_PENALTY=1.595076 PRIMER_LEFT_8_SEQUENCE=CCTGATAGAAACGCCAACTCA PRIMER_RIGHT_8_SEQUENCE=TGAGTTGGCGTTTCTATCAGG PRIMER_INTERNAL_8_SEQUENCE=AAAACGCAAAGCACGCTCCCG PRIMER_LEFT_8=56,21 PRIMER_RIGHT_8=76,21 PRIMER_INTERNAL_8=123,21 PRIMER_LEFT_8_TM=58.027 PRIMER_RIGHT_8_TM=58.027 PRIMER_INTERNAL_8_TM=59.405 PRIMER_LEFT_8_GC_PERCENT=47.619 PRIMER_RIGHT_8_GC_PERCENT=47.619 PRIMER_INTERNAL_8_GC_PERCENT=57.143 PRIMER_INTERNAL_8_SELF_ANY_TH=1.50 PRIMER_LEFT_8_SELF_ANY_TH=0.00 PRIMER_RIGHT_8_SELF_ANY_TH=0.00 PRIMER_INTERNAL_8_SELF_END_TH=1.50 PRIMER_LEFT_8_SELF_END_TH=0.00 PRIMER_RIGHT_8_SELF_END_TH=0.00 PRIMER_LEFT_8_HAIRPIN_TH=0.00 PRIMER_RIGHT_8_HAIRPIN_TH=0.00 PRIMER_INTERNAL_8_HAIRPIN_TH=41.14 PRIMER_LEFT_8_LIBRARY_MISPRIMING=10.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1) PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene PRIMER_LEFT_8_END_STABILITY=3.4100 PRIMER_RIGHT_8_END_STABILITY=3.8600 PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=20.8283 PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=12.8893 PRIMER_LEFT_9_PENALTY=1.973945 PRIMER_RIGHT_9_PENALTY=1.973945 PRIMER_INTERNAL_9_PENALTY=2.007622 PRIMER_LEFT_9_SEQUENCE=ACCTGATAGAAACGCCAACTC PRIMER_RIGHT_9_SEQUENCE=GAGTTGGCGTTTCTATCAGGT PRIMER_INTERNAL_9_SEQUENCE=CCAAAACGCAAAGCACGCTCCC PRIMER_LEFT_9=55,21 PRIMER_RIGHT_9=75,21 PRIMER_INTERNAL_9=121,22 PRIMER_LEFT_9_TM=58.026 PRIMER_RIGHT_9_TM=58.026 PRIMER_INTERNAL_9_TM=59.992 PRIMER_LEFT_9_GC_PERCENT=47.619 PRIMER_RIGHT_9_GC_PERCENT=47.619 PRIMER_INTERNAL_9_GC_PERCENT=59.091 PRIMER_INTERNAL_9_SELF_ANY_TH=0.00 PRIMER_LEFT_9_SELF_ANY_TH=0.00 PRIMER_RIGHT_9_SELF_ANY_TH=0.00 PRIMER_INTERNAL_9_SELF_END_TH=0.00 PRIMER_LEFT_9_SELF_END_TH=0.00 PRIMER_RIGHT_9_SELF_END_TH=0.00 PRIMER_LEFT_9_HAIRPIN_TH=0.00 PRIMER_RIGHT_9_HAIRPIN_TH=0.00 PRIMER_INTERNAL_9_HAIRPIN_TH=0.00 PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_9_END_STABILITY=3.0100 PRIMER_RIGHT_9_END_STABILITY=4.0000 PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=6.8289 PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=11.0226 PRIMER_LEFT_10_PENALTY=1.973945 PRIMER_RIGHT_10_PENALTY=1.973945 PRIMER_INTERNAL_10_PENALTY=2.235780 PRIMER_LEFT_10_SEQUENCE=GACCTGATAGAAACGCCAACT PRIMER_RIGHT_10_SEQUENCE=AGTTGGCGTTTCTATCAGGTC PRIMER_INTERNAL_10_SEQUENCE=AACGCAAAGCACGCTCCCG PRIMER_LEFT_10=54,21 PRIMER_RIGHT_10=74,21 PRIMER_INTERNAL_10=125,19 PRIMER_LEFT_10_TM=58.026 PRIMER_RIGHT_10_TM=58.026 PRIMER_INTERNAL_10_TM=58.764 PRIMER_LEFT_10_GC_PERCENT=47.619 PRIMER_RIGHT_10_GC_PERCENT=47.619 PRIMER_INTERNAL_10_GC_PERCENT=63.158 PRIMER_INTERNAL_10_SELF_ANY_TH=1.50 PRIMER_LEFT_10_SELF_ANY_TH=0.00 PRIMER_RIGHT_10_SELF_ANY_TH=0.00 PRIMER_INTERNAL_10_SELF_END_TH=1.50 PRIMER_LEFT_10_SELF_END_TH=0.00 PRIMER_RIGHT_10_SELF_END_TH=0.00 PRIMER_LEFT_10_HAIRPIN_TH=0.00 PRIMER_RIGHT_10_HAIRPIN_TH=0.00 PRIMER_INTERNAL_10_HAIRPIN_TH=41.14 PRIMER_LEFT_10_LIBRARY_MISPRIMING=10.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_10_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_10_END_STABILITY=3.1600 PRIMER_RIGHT_10_END_STABILITY=3.8500 PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=3.2290 PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=8.6567 PRIMER_LEFT_11_PENALTY=2.120321 PRIMER_RIGHT_11_PENALTY=2.120321 PRIMER_INTERNAL_11_PENALTY=2.240074 PRIMER_LEFT_11_SEQUENCE=AAACGCCAACTCACTGTATGA PRIMER_RIGHT_11_SEQUENCE=TCATACAGTGAGTTGGCGTTT PRIMER_INTERNAL_11_SEQUENCE=AAACGCAAAGCACGCTCCCGAT PRIMER_LEFT_11=64,21 PRIMER_RIGHT_11=84,21 PRIMER_INTERNAL_11=124,22 PRIMER_LEFT_11_TM=57.880 PRIMER_RIGHT_11_TM=57.880 PRIMER_INTERNAL_11_TM=60.240 PRIMER_LEFT_11_GC_PERCENT=42.857 PRIMER_RIGHT_11_GC_PERCENT=42.857 PRIMER_INTERNAL_11_GC_PERCENT=54.545 PRIMER_INTERNAL_11_SELF_ANY_TH=7.82 PRIMER_LEFT_11_SELF_ANY_TH=0.00 PRIMER_RIGHT_11_SELF_ANY_TH=0.00 PRIMER_INTERNAL_11_SELF_END_TH=0.00 PRIMER_LEFT_11_SELF_END_TH=0.00 PRIMER_RIGHT_11_SELF_END_TH=0.00 PRIMER_LEFT_11_HAIRPIN_TH=35.45 PRIMER_RIGHT_11_HAIRPIN_TH=31.80 PRIMER_INTERNAL_11_HAIRPIN_TH=36.42 PRIMER_LEFT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_LEFT_11_END_STABILITY=2.1500 PRIMER_RIGHT_11_END_STABILITY=3.6000 PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=5.5045 PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=24.8608 PRIMER_LEFT_12_PENALTY=2.142778 PRIMER_RIGHT_12_PENALTY=2.142778 PRIMER_INTERNAL_12_PENALTY=2.299276 PRIMER_LEFT_12_SEQUENCE=TTCCAAAACGCAAAGCACGC PRIMER_RIGHT_12_SEQUENCE=GCGTGCTTTGCGTTTTGGAA PRIMER_INTERNAL_12_SEQUENCE=CAAAACGCAAAGCACGCTCCCG PRIMER_LEFT_12=119,20 PRIMER_RIGHT_12=138,20 PRIMER_INTERNAL_12=122,22 PRIMER_LEFT_12_TM=61.143 PRIMER_RIGHT_12_TM=61.143 PRIMER_INTERNAL_12_TM=60.299 PRIMER_LEFT_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_GC_PERCENT=50.000 PRIMER_INTERNAL_12_GC_PERCENT=59.091 PRIMER_INTERNAL_12_SELF_ANY_TH=1.50 PRIMER_LEFT_12_SELF_ANY_TH=0.00 PRIMER_RIGHT_12_SELF_ANY_TH=0.00 PRIMER_INTERNAL_12_SELF_END_TH=1.50 PRIMER_LEFT_12_SELF_END_TH=0.00 PRIMER_RIGHT_12_SELF_END_TH=0.00 PRIMER_LEFT_12_HAIRPIN_TH=0.00 PRIMER_RIGHT_12_HAIRPIN_TH=0.00 PRIMER_INTERNAL_12_HAIRPIN_TH=41.14 PRIMER_LEFT_12_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner PRIMER_RIGHT_12_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_12_END_STABILITY=5.3400 PRIMER_RIGHT_12_END_STABILITY=3.5300 PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=6.5778 PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=7.7746 PRIMER_LEFT_13_PENALTY=2.299089 PRIMER_RIGHT_13_PENALTY=2.299089 PRIMER_INTERNAL_13_PENALTY=2.427391 PRIMER_LEFT_13_SEQUENCE=AAAACGCAAAGCACGCTCC PRIMER_RIGHT_13_SEQUENCE=GGAGCGTGCTTTGCGTTTT PRIMER_INTERNAL_13_SEQUENCE=AAAACGCAAAGCACGCTCCCGA PRIMER_LEFT_13=123,19 PRIMER_RIGHT_13=141,19 PRIMER_INTERNAL_13=123,22 PRIMER_LEFT_13_TM=60.299 PRIMER_RIGHT_13_TM=60.299 PRIMER_INTERNAL_13_TM=60.427 PRIMER_LEFT_13_GC_PERCENT=52.632 PRIMER_RIGHT_13_GC_PERCENT=52.632 PRIMER_INTERNAL_13_GC_PERCENT=54.545 PRIMER_INTERNAL_13_SELF_ANY_TH=7.82 PRIMER_LEFT_13_SELF_ANY_TH=0.00 PRIMER_RIGHT_13_SELF_ANY_TH=0.00 PRIMER_INTERNAL_13_SELF_END_TH=0.00 PRIMER_LEFT_13_SELF_END_TH=0.00 PRIMER_RIGHT_13_SELF_END_TH=0.00 PRIMER_LEFT_13_HAIRPIN_TH=0.00 PRIMER_RIGHT_13_HAIRPIN_TH=37.44 PRIMER_INTERNAL_13_HAIRPIN_TH=36.42 PRIMER_LEFT_13_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_13_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_13_END_STABILITY=4.7000 PRIMER_RIGHT_13_END_STABILITY=2.4300 PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=0.4266 PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=8.7659 PRIMER_LEFT_14_PENALTY=2.402628 PRIMER_RIGHT_14_PENALTY=2.402628 PRIMER_INTERNAL_14_PENALTY=2.610811 PRIMER_LEFT_14_SEQUENCE=AGAAACGCCAACTCACTGTAT PRIMER_RIGHT_14_SEQUENCE=ATACAGTGAGTTGGCGTTTCT PRIMER_INTERNAL_14_SEQUENCE=AACGCAAAGCACGCTCCCGATC PRIMER_LEFT_14=62,21 PRIMER_RIGHT_14=82,21 PRIMER_INTERNAL_14=125,22 PRIMER_LEFT_14_TM=57.597 PRIMER_RIGHT_14_TM=57.597 PRIMER_INTERNAL_14_TM=60.611 PRIMER_LEFT_14_GC_PERCENT=42.857 PRIMER_RIGHT_14_GC_PERCENT=42.857 PRIMER_INTERNAL_14_GC_PERCENT=59.091 PRIMER_INTERNAL_14_SELF_ANY_TH=7.82 PRIMER_LEFT_14_SELF_ANY_TH=0.00 PRIMER_RIGHT_14_SELF_ANY_TH=0.00 PRIMER_INTERNAL_14_SELF_END_TH=0.00 PRIMER_LEFT_14_SELF_END_TH=0.00 PRIMER_RIGHT_14_SELF_END_TH=0.00 PRIMER_LEFT_14_HAIRPIN_TH=0.00 PRIMER_RIGHT_14_HAIRPIN_TH=0.00 PRIMER_INTERNAL_14_HAIRPIN_TH=36.42 PRIMER_LEFT_14_LIBRARY_MISPRIMING=11.00, reverse MARINER1 Human mariner PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_14_END_STABILITY=2.2900 PRIMER_RIGHT_14_END_STABILITY=2.5200 PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=16.5430 PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=19.2811 PRIMER_LEFT_15_PENALTY=2.402628 PRIMER_RIGHT_15_PENALTY=2.402628 PRIMER_INTERNAL_15_PENALTY=2.740095 PRIMER_LEFT_15_SEQUENCE=ATAGAAACGCCAACTCACTGT PRIMER_RIGHT_15_SEQUENCE=ACAGTGAGTTGGCGTTTCTAT PRIMER_INTERNAL_15_SEQUENCE=CGCAAAGCACGCTCCCGAT PRIMER_LEFT_15=60,21 PRIMER_RIGHT_15=80,21 PRIMER_INTERNAL_15=127,19 PRIMER_LEFT_15_TM=57.597 PRIMER_RIGHT_15_TM=57.597 PRIMER_INTERNAL_15_TM=58.260 PRIMER_LEFT_15_GC_PERCENT=42.857 PRIMER_RIGHT_15_GC_PERCENT=42.857 PRIMER_INTERNAL_15_GC_PERCENT=63.158 PRIMER_INTERNAL_15_SELF_ANY_TH=0.00 PRIMER_LEFT_15_SELF_ANY_TH=0.00 PRIMER_RIGHT_15_SELF_ANY_TH=0.00 PRIMER_INTERNAL_15_SELF_END_TH=0.00 PRIMER_LEFT_15_SELF_END_TH=0.00 PRIMER_RIGHT_15_SELF_END_TH=0.00 PRIMER_LEFT_15_HAIRPIN_TH=0.00 PRIMER_RIGHT_15_HAIRPIN_TH=0.00 PRIMER_INTERNAL_15_HAIRPIN_TH=36.42 PRIMER_LEFT_15_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_15_END_STABILITY=3.5500 PRIMER_RIGHT_15_END_STABILITY=1.9800 PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=14.5010 PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=21.0214 PRIMER_LEFT_16_PENALTY=2.455619 PRIMER_RIGHT_16_PENALTY=2.455619 PRIMER_INTERNAL_16_PENALTY=2.880381 PRIMER_LEFT_16_SEQUENCE=GAAACGCCAACTCACTGTATG PRIMER_RIGHT_16_SEQUENCE=CATACAGTGAGTTGGCGTTTC PRIMER_INTERNAL_16_SEQUENCE=TCCAAAACGCAAAGCACGCTCC PRIMER_LEFT_16=63,21 PRIMER_RIGHT_16=83,21 PRIMER_INTERNAL_16=120,22 PRIMER_LEFT_16_TM=57.544 PRIMER_RIGHT_16_TM=57.544 PRIMER_INTERNAL_16_TM=59.120 PRIMER_LEFT_16_GC_PERCENT=47.619 PRIMER_RIGHT_16_GC_PERCENT=47.619 PRIMER_INTERNAL_16_GC_PERCENT=54.545 PRIMER_INTERNAL_16_SELF_ANY_TH=0.00 PRIMER_LEFT_16_SELF_ANY_TH=0.00 PRIMER_RIGHT_16_SELF_ANY_TH=0.00 PRIMER_INTERNAL_16_SELF_END_TH=0.00 PRIMER_LEFT_16_SELF_END_TH=0.00 PRIMER_RIGHT_16_SELF_END_TH=0.00 PRIMER_LEFT_16_HAIRPIN_TH=0.00 PRIMER_RIGHT_16_HAIRPIN_TH=0.00 PRIMER_INTERNAL_16_HAIRPIN_TH=0.00 PRIMER_LEFT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner PRIMER_RIGHT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner PRIMER_LEFT_16_END_STABILITY=2.3900 PRIMER_RIGHT_16_END_STABILITY=2.7800 PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=9.1798 PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=18.8273 PRIMER_LEFT_17_PENALTY=2.539776 PRIMER_RIGHT_17_PENALTY=2.539776 PRIMER_INTERNAL_17_PENALTY=2.939429 PRIMER_LEFT_17_SEQUENCE=CAAAACGCAAAGCACGCTCC PRIMER_RIGHT_17_SEQUENCE=GGAGCGTGCTTTGCGTTTTG PRIMER_INTERNAL_17_SEQUENCE=CAAAACGCAAAGCACGCTCCC PRIMER_LEFT_17=122,20 PRIMER_RIGHT_17=141,20 PRIMER_INTERNAL_17=122,21 PRIMER_LEFT_17_TM=61.540 PRIMER_RIGHT_17_TM=61.540 PRIMER_INTERNAL_17_TM=58.061 PRIMER_LEFT_17_GC_PERCENT=55.000 PRIMER_RIGHT_17_GC_PERCENT=55.000 PRIMER_INTERNAL_17_GC_PERCENT=57.143 PRIMER_INTERNAL_17_SELF_ANY_TH=0.00 PRIMER_LEFT_17_SELF_ANY_TH=0.00 PRIMER_RIGHT_17_SELF_ANY_TH=0.00 PRIMER_INTERNAL_17_SELF_END_TH=0.00 PRIMER_LEFT_17_SELF_END_TH=0.00 PRIMER_RIGHT_17_SELF_END_TH=0.00 PRIMER_LEFT_17_HAIRPIN_TH=0.00 PRIMER_RIGHT_17_HAIRPIN_TH=37.44 PRIMER_INTERNAL_17_HAIRPIN_TH=0.00 PRIMER_LEFT_17_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_17_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_17_END_STABILITY=4.7000 PRIMER_RIGHT_17_END_STABILITY=2.4400 PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=4.7389 PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=6.1004 PRIMER_LEFT_18_PENALTY=2.539776 PRIMER_RIGHT_18_PENALTY=2.539776 PRIMER_INTERNAL_18_PENALTY=2.939429 PRIMER_LEFT_18_SEQUENCE=CCAAAACGCAAAGCACGCTC PRIMER_RIGHT_18_SEQUENCE=GAGCGTGCTTTGCGTTTTGG PRIMER_INTERNAL_18_SEQUENCE=CCAAAACGCAAAGCACGCTCC PRIMER_LEFT_18=121,20 PRIMER_RIGHT_18=140,20 PRIMER_INTERNAL_18=121,21 PRIMER_LEFT_18_TM=61.540 PRIMER_RIGHT_18_TM=61.540 PRIMER_INTERNAL_18_TM=58.061 PRIMER_LEFT_18_GC_PERCENT=55.000 PRIMER_RIGHT_18_GC_PERCENT=55.000 PRIMER_INTERNAL_18_GC_PERCENT=57.143 PRIMER_INTERNAL_18_SELF_ANY_TH=0.00 PRIMER_LEFT_18_SELF_ANY_TH=0.00 PRIMER_RIGHT_18_SELF_ANY_TH=0.00 PRIMER_INTERNAL_18_SELF_END_TH=0.00 PRIMER_LEFT_18_SELF_END_TH=0.00 PRIMER_RIGHT_18_SELF_END_TH=0.00 PRIMER_LEFT_18_HAIRPIN_TH=0.00 PRIMER_RIGHT_18_HAIRPIN_TH=37.44 PRIMER_INTERNAL_18_HAIRPIN_TH=0.00 PRIMER_LEFT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_RIGHT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_18_END_STABILITY=5.0300 PRIMER_RIGHT_18_END_STABILITY=3.2800 PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=7.7786 PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=9.7248 PRIMER_LEFT_19_PENALTY=2.587613 PRIMER_RIGHT_19_PENALTY=2.587613 PRIMER_INTERNAL_19_PENALTY=3.476553 PRIMER_LEFT_19_SEQUENCE=TCCAAAACGCAAAGCACGC PRIMER_RIGHT_19_SEQUENCE=GCGTGCTTTGCGTTTTGGA PRIMER_INTERNAL_19_SEQUENCE=AAAACGCAAAGCACGCTCCCGAT PRIMER_LEFT_19=120,19 PRIMER_RIGHT_19=138,19 PRIMER_INTERNAL_19=123,23 PRIMER_LEFT_19_TM=60.588 PRIMER_RIGHT_19_TM=60.588 PRIMER_INTERNAL_19_TM=60.477 PRIMER_LEFT_19_GC_PERCENT=52.632 PRIMER_RIGHT_19_GC_PERCENT=52.632 PRIMER_INTERNAL_19_GC_PERCENT=52.174 PRIMER_INTERNAL_19_SELF_ANY_TH=7.82 PRIMER_LEFT_19_SELF_ANY_TH=0.00 PRIMER_RIGHT_19_SELF_ANY_TH=0.00 PRIMER_INTERNAL_19_SELF_END_TH=0.00 PRIMER_LEFT_19_SELF_END_TH=0.00 PRIMER_RIGHT_19_SELF_END_TH=0.00 PRIMER_LEFT_19_HAIRPIN_TH=0.00 PRIMER_RIGHT_19_HAIRPIN_TH=0.00 PRIMER_INTERNAL_19_HAIRPIN_TH=36.42 PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner PRIMER_RIGHT_19_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_19_END_STABILITY=5.3400 PRIMER_RIGHT_19_END_STABILITY=3.5300 PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=6.3533 PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=6.8599 PRIMER_LEFT_20_PENALTY=2.588667 PRIMER_RIGHT_20_PENALTY=2.588667 PRIMER_INTERNAL_20_PENALTY=3.598972 PRIMER_LEFT_20_SEQUENCE=CCAAAACGCAAAGCACGCT PRIMER_RIGHT_20_SEQUENCE=AGCGTGCTTTGCGTTTTGG PRIMER_INTERNAL_20_SEQUENCE=TTCCAAAACGCAAAGCACGCTCC PRIMER_LEFT_20=121,19 PRIMER_RIGHT_20=139,19 PRIMER_INTERNAL_20=119,23 PRIMER_LEFT_20_TM=60.589 PRIMER_RIGHT_20_TM=60.589 PRIMER_INTERNAL_20_TM=59.401 PRIMER_LEFT_20_GC_PERCENT=52.632 PRIMER_RIGHT_20_GC_PERCENT=52.632 PRIMER_INTERNAL_20_GC_PERCENT=52.174 PRIMER_INTERNAL_20_SELF_ANY_TH=0.00 PRIMER_LEFT_20_SELF_ANY_TH=0.00 PRIMER_RIGHT_20_SELF_ANY_TH=0.00 PRIMER_INTERNAL_20_SELF_END_TH=0.00 PRIMER_LEFT_20_SELF_END_TH=0.00 PRIMER_RIGHT_20_SELF_END_TH=0.00 PRIMER_LEFT_20_HAIRPIN_TH=0.00 PRIMER_RIGHT_20_HAIRPIN_TH=37.44 PRIMER_INTERNAL_20_HAIRPIN_TH=0.00 PRIMER_LEFT_20_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_20_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner PRIMER_LEFT_20_END_STABILITY=5.0700 PRIMER_RIGHT_20_END_STABILITY=3.2800 PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=7.2070 PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=12.0561 PRIMER_LEFT_21_PENALTY=2.627395 PRIMER_RIGHT_21_PENALTY=2.627395 PRIMER_INTERNAL_21_PENALTY=3.606233 PRIMER_LEFT_21_SEQUENCE=ATGACCTGATAGAAACGCCAA PRIMER_RIGHT_21_SEQUENCE=TTGGCGTTTCTATCAGGTCAT PRIMER_INTERNAL_21_SEQUENCE=ACGCAAAGCACGCTCCCG PRIMER_LEFT_21=52,21 PRIMER_RIGHT_21=72,21 PRIMER_INTERNAL_21=126,18 PRIMER_LEFT_21_TM=57.373 PRIMER_RIGHT_21_TM=57.373 PRIMER_INTERNAL_21_TM=58.394 PRIMER_LEFT_21_GC_PERCENT=42.857 PRIMER_RIGHT_21_GC_PERCENT=42.857 PRIMER_INTERNAL_21_GC_PERCENT=66.667 PRIMER_INTERNAL_21_SELF_ANY_TH=1.50 PRIMER_LEFT_21_SELF_ANY_TH=0.00 PRIMER_RIGHT_21_SELF_ANY_TH=0.00 PRIMER_INTERNAL_21_SELF_END_TH=1.50 PRIMER_LEFT_21_SELF_END_TH=0.00 PRIMER_RIGHT_21_SELF_END_TH=0.00 PRIMER_LEFT_21_HAIRPIN_TH=0.00 PRIMER_RIGHT_21_HAIRPIN_TH=0.00 PRIMER_INTERNAL_21_HAIRPIN_TH=41.14 PRIMER_LEFT_21_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_21_END_STABILITY=4.5200 PRIMER_RIGHT_21_END_STABILITY=3.0600 PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=3.5472 PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=0.2868 PRIMER_LEFT_22_PENALTY=2.678056 PRIMER_RIGHT_22_PENALTY=2.678056 PRIMER_INTERNAL_22_PENALTY=3.829005 PRIMER_LEFT_22_SEQUENCE=TGATAGAAACGCCAACTCACT PRIMER_RIGHT_22_SEQUENCE=AGTGAGTTGGCGTTTCTATCA PRIMER_INTERNAL_22_SEQUENCE=AAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_22=58,21 PRIMER_RIGHT_22=78,21 PRIMER_INTERNAL_22=124,23 PRIMER_LEFT_22_TM=57.322 PRIMER_RIGHT_22_TM=57.322 PRIMER_INTERNAL_22_TM=60.829 PRIMER_LEFT_22_GC_PERCENT=42.857 PRIMER_RIGHT_22_GC_PERCENT=42.857 PRIMER_INTERNAL_22_GC_PERCENT=56.522 PRIMER_INTERNAL_22_SELF_ANY_TH=7.82 PRIMER_LEFT_22_SELF_ANY_TH=0.00 PRIMER_RIGHT_22_SELF_ANY_TH=0.00 PRIMER_INTERNAL_22_SELF_END_TH=0.00 PRIMER_LEFT_22_SELF_END_TH=0.00 PRIMER_RIGHT_22_SELF_END_TH=0.00 PRIMER_LEFT_22_HAIRPIN_TH=0.00 PRIMER_RIGHT_22_HAIRPIN_TH=0.00 PRIMER_INTERNAL_22_HAIRPIN_TH=36.42 PRIMER_LEFT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_22_END_STABILITY=3.4100 PRIMER_RIGHT_22_END_STABILITY=2.1500 PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=16.4194 PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=13.4901 PRIMER_LEFT_23_PENALTY=2.780624 PRIMER_RIGHT_23_PENALTY=2.780624 PRIMER_INTERNAL_23_PENALTY=3.865064 PRIMER_LEFT_23_SEQUENCE=AGAAACGCCAACTCACTGT PRIMER_RIGHT_23_SEQUENCE=ACAGTGAGTTGGCGTTTCT PRIMER_INTERNAL_23_SEQUENCE=TCCAAAACGCAAAGCACGCTC PRIMER_LEFT_23=62,19 PRIMER_RIGHT_23=80,19 PRIMER_INTERNAL_23=120,21 PRIMER_LEFT_23_TM=57.219 PRIMER_RIGHT_23_TM=57.219 PRIMER_INTERNAL_23_TM=57.135 PRIMER_LEFT_23_GC_PERCENT=47.368 PRIMER_RIGHT_23_GC_PERCENT=47.368 PRIMER_INTERNAL_23_GC_PERCENT=52.381 PRIMER_INTERNAL_23_SELF_ANY_TH=0.00 PRIMER_LEFT_23_SELF_ANY_TH=0.00 PRIMER_RIGHT_23_SELF_ANY_TH=0.00 PRIMER_INTERNAL_23_SELF_END_TH=0.00 PRIMER_LEFT_23_SELF_END_TH=0.00 PRIMER_RIGHT_23_SELF_END_TH=0.00 PRIMER_LEFT_23_HAIRPIN_TH=0.00 PRIMER_RIGHT_23_HAIRPIN_TH=0.00 PRIMER_INTERNAL_23_HAIRPIN_TH=0.00 PRIMER_LEFT_23_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_23_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_23_END_STABILITY=3.5500 PRIMER_RIGHT_23_END_STABILITY=2.5200 PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=8.5732 PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=19.2811 PRIMER_LEFT_24_PENALTY=2.999071 PRIMER_RIGHT_24_PENALTY=2.999071 PRIMER_INTERNAL_24_PENALTY=3.905453 PRIMER_LEFT_24_SEQUENCE=TCCAAAACGCAAAGCACGCT PRIMER_RIGHT_24_SEQUENCE=AGCGTGCTTTGCGTTTTGGA PRIMER_INTERNAL_24_SEQUENCE=CGCAAAGCACGCTCCCGA PRIMER_LEFT_24=120,20 PRIMER_RIGHT_24=139,20 PRIMER_INTERNAL_24=127,18 PRIMER_LEFT_24_TM=61.999 PRIMER_RIGHT_24_TM=61.999 PRIMER_INTERNAL_24_TM=58.095 PRIMER_LEFT_24_GC_PERCENT=50.000 PRIMER_RIGHT_24_GC_PERCENT=50.000 PRIMER_INTERNAL_24_GC_PERCENT=66.667 PRIMER_INTERNAL_24_SELF_ANY_TH=0.00 PRIMER_LEFT_24_SELF_ANY_TH=0.00 PRIMER_RIGHT_24_SELF_ANY_TH=0.00 PRIMER_INTERNAL_24_SELF_END_TH=0.00 PRIMER_LEFT_24_SELF_END_TH=0.00 PRIMER_RIGHT_24_SELF_END_TH=0.00 PRIMER_LEFT_24_HAIRPIN_TH=0.00 PRIMER_RIGHT_24_HAIRPIN_TH=37.44 PRIMER_INTERNAL_24_HAIRPIN_TH=36.42 PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_24_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_24_END_STABILITY=5.0700 PRIMER_RIGHT_24_END_STABILITY=3.5300 PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=8.9249 PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=6.8599 PRIMER_LEFT_25_PENALTY=2.999229 PRIMER_RIGHT_25_PENALTY=2.999229 PRIMER_INTERNAL_25_PENALTY=3.954555 PRIMER_LEFT_25_SEQUENCE=GATAGAAACGCCAACTCACTG PRIMER_RIGHT_25_SEQUENCE=CAGTGAGTTGGCGTTTCTATC PRIMER_INTERNAL_25_SEQUENCE=TCCAAAACGCAAAGCACGCTCCC PRIMER_LEFT_25=59,21 PRIMER_RIGHT_25=79,21 PRIMER_INTERNAL_25=120,23 PRIMER_LEFT_25_TM=57.001 PRIMER_RIGHT_25_TM=57.001 PRIMER_INTERNAL_25_TM=60.955 PRIMER_LEFT_25_GC_PERCENT=47.619 PRIMER_RIGHT_25_GC_PERCENT=47.619 PRIMER_INTERNAL_25_GC_PERCENT=56.522 PRIMER_INTERNAL_25_SELF_ANY_TH=0.00 PRIMER_LEFT_25_SELF_ANY_TH=0.00 PRIMER_RIGHT_25_SELF_ANY_TH=0.00 PRIMER_INTERNAL_25_SELF_END_TH=0.00 PRIMER_LEFT_25_SELF_END_TH=0.00 PRIMER_RIGHT_25_SELF_END_TH=0.00 PRIMER_LEFT_25_HAIRPIN_TH=0.00 PRIMER_RIGHT_25_HAIRPIN_TH=0.00 PRIMER_INTERNAL_25_HAIRPIN_TH=0.00 PRIMER_LEFT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_25_END_STABILITY=3.6600 PRIMER_RIGHT_25_END_STABILITY=2.0800 PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=19.5396 PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=18.4032 PRIMER_LEFT_26_PENALTY=2.999229 PRIMER_RIGHT_26_PENALTY=2.999229 PRIMER_INTERNAL_26_PENALTY=4.240075 PRIMER_LEFT_26_SEQUENCE=CTGATAGAAACGCCAACTCAC PRIMER_RIGHT_26_SEQUENCE=GTGAGTTGGCGTTTCTATCAG PRIMER_INTERNAL_26_SEQUENCE=CAAAACGCAAAGCACGCTCCCGA PRIMER_LEFT_26=57,21 PRIMER_RIGHT_26=77,21 PRIMER_INTERNAL_26=122,23 PRIMER_LEFT_26_TM=57.001 PRIMER_RIGHT_26_TM=57.001 PRIMER_INTERNAL_26_TM=61.240 PRIMER_LEFT_26_GC_PERCENT=47.619 PRIMER_RIGHT_26_GC_PERCENT=47.619 PRIMER_INTERNAL_26_GC_PERCENT=56.522 PRIMER_INTERNAL_26_SELF_ANY_TH=7.82 PRIMER_LEFT_26_SELF_ANY_TH=0.00 PRIMER_RIGHT_26_SELF_ANY_TH=0.00 PRIMER_INTERNAL_26_SELF_END_TH=0.00 PRIMER_LEFT_26_SELF_END_TH=0.00 PRIMER_RIGHT_26_SELF_END_TH=0.00 PRIMER_LEFT_26_HAIRPIN_TH=0.00 PRIMER_RIGHT_26_HAIRPIN_TH=0.00 PRIMER_INTERNAL_26_HAIRPIN_TH=36.42 PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_26_END_STABILITY=3.5100 PRIMER_RIGHT_26_END_STABILITY=2.9000 PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=13.7640 PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=15.9212 PRIMER_LEFT_27_PENALTY=3.455227 PRIMER_RIGHT_27_PENALTY=3.455227 PRIMER_INTERNAL_27_PENALTY=4.486709 PRIMER_LEFT_27_SEQUENCE=ATTCCAAAACGCAAAGCACGC PRIMER_RIGHT_27_SEQUENCE=GCGTGCTTTGCGTTTTGGAAT PRIMER_INTERNAL_27_SEQUENCE=TTCCAAAACGCAAAGCACGCTC PRIMER_LEFT_27=118,21 PRIMER_RIGHT_27=138,21 PRIMER_INTERNAL_27=119,22 PRIMER_LEFT_27_TM=61.455 PRIMER_RIGHT_27_TM=61.455 PRIMER_INTERNAL_27_TM=57.513 PRIMER_LEFT_27_GC_PERCENT=47.619 PRIMER_RIGHT_27_GC_PERCENT=47.619 PRIMER_INTERNAL_27_GC_PERCENT=50.000 PRIMER_INTERNAL_27_SELF_ANY_TH=0.00 PRIMER_LEFT_27_SELF_ANY_TH=0.00 PRIMER_RIGHT_27_SELF_ANY_TH=0.00 PRIMER_INTERNAL_27_SELF_END_TH=0.00 PRIMER_LEFT_27_SELF_END_TH=0.00 PRIMER_RIGHT_27_SELF_END_TH=0.00 PRIMER_LEFT_27_HAIRPIN_TH=0.00 PRIMER_RIGHT_27_HAIRPIN_TH=0.00 PRIMER_INTERNAL_27_HAIRPIN_TH=0.00 PRIMER_LEFT_27_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon PRIMER_LEFT_27_END_STABILITY=5.3400 PRIMER_RIGHT_27_END_STABILITY=3.0100 PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=1.8923 PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=11.3682 PRIMER_LEFT_28_PENALTY=3.677573 PRIMER_RIGHT_28_PENALTY=3.677573 PRIMER_INTERNAL_28_PENALTY=4.513970 PRIMER_LEFT_28_SEQUENCE=AAAACGCAAAGCACGCTCCC PRIMER_RIGHT_28_SEQUENCE=GGGAGCGTGCTTTGCGTTTT PRIMER_INTERNAL_28_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCC PRIMER_LEFT_28=123,20 PRIMER_RIGHT_28=142,20 PRIMER_INTERNAL_28=118,24 PRIMER_LEFT_28_TM=62.678 PRIMER_RIGHT_28_TM=62.678 PRIMER_INTERNAL_28_TM=59.486 PRIMER_LEFT_28_GC_PERCENT=55.000 PRIMER_RIGHT_28_GC_PERCENT=55.000 PRIMER_INTERNAL_28_GC_PERCENT=50.000 PRIMER_INTERNAL_28_SELF_ANY_TH=0.00 PRIMER_LEFT_28_SELF_ANY_TH=0.00 PRIMER_RIGHT_28_SELF_ANY_TH=0.00 PRIMER_INTERNAL_28_SELF_END_TH=0.00 PRIMER_LEFT_28_SELF_END_TH=0.00 PRIMER_RIGHT_28_SELF_END_TH=0.00 PRIMER_LEFT_28_HAIRPIN_TH=0.00 PRIMER_RIGHT_28_HAIRPIN_TH=37.44 PRIMER_INTERNAL_28_HAIRPIN_TH=0.00 PRIMER_LEFT_28_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1) PRIMER_RIGHT_28_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_28_END_STABILITY=4.3000 PRIMER_RIGHT_28_END_STABILITY=2.4300 PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=0.0612 PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=1.5624 PRIMER_LEFT_29_PENALTY=4.100862 PRIMER_RIGHT_29_PENALTY=4.100862 PRIMER_INTERNAL_29_PENALTY=5.029817 PRIMER_LEFT_29_SEQUENCE=GCAAAGCACGCTCCCGATC PRIMER_RIGHT_29_SEQUENCE=GATCGGGAGCGTGCTTTGC PRIMER_INTERNAL_29_SEQUENCE=AAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_29=128,19 PRIMER_RIGHT_29=146,19 PRIMER_INTERNAL_29=123,24 PRIMER_LEFT_29_TM=62.101 PRIMER_RIGHT_29_TM=62.101 PRIMER_INTERNAL_29_TM=61.030 PRIMER_LEFT_29_GC_PERCENT=63.158 PRIMER_RIGHT_29_GC_PERCENT=63.158 PRIMER_INTERNAL_29_GC_PERCENT=54.167 PRIMER_INTERNAL_29_SELF_ANY_TH=0.00 PRIMER_LEFT_29_SELF_ANY_TH=0.00 PRIMER_RIGHT_29_SELF_ANY_TH=0.00 PRIMER_INTERNAL_29_SELF_END_TH=0.00 PRIMER_LEFT_29_SELF_END_TH=0.00 PRIMER_RIGHT_29_SELF_END_TH=0.00 PRIMER_LEFT_29_HAIRPIN_TH=38.29 PRIMER_RIGHT_29_HAIRPIN_TH=0.00 PRIMER_INTERNAL_29_HAIRPIN_TH=36.42 PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon PRIMER_LEFT_29_END_STABILITY=3.6900 PRIMER_RIGHT_29_END_STABILITY=3.6800 PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=5.4030 PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_30_PENALTY=4.194272 PRIMER_RIGHT_30_PENALTY=4.194272 PRIMER_INTERNAL_30_PENALTY=5.052238 PRIMER_LEFT_30_SEQUENCE=AAACGCAAAGCACGCTCCC PRIMER_RIGHT_30_SEQUENCE=GGGAGCGTGCTTTGCGTTT PRIMER_INTERNAL_30_SEQUENCE=CCAAAACGCAAAGCACGCTCCCG PRIMER_LEFT_30=124,19 PRIMER_RIGHT_30=142,19 PRIMER_INTERNAL_30=121,23 PRIMER_LEFT_30_TM=62.194 PRIMER_RIGHT_30_TM=62.194 PRIMER_INTERNAL_30_TM=62.052 PRIMER_LEFT_30_GC_PERCENT=57.895 PRIMER_RIGHT_30_GC_PERCENT=57.895 PRIMER_INTERNAL_30_GC_PERCENT=60.870 PRIMER_INTERNAL_30_SELF_ANY_TH=1.50 PRIMER_LEFT_30_SELF_ANY_TH=0.00 PRIMER_RIGHT_30_SELF_ANY_TH=0.00 PRIMER_INTERNAL_30_SELF_END_TH=1.50 PRIMER_LEFT_30_SELF_END_TH=0.00 PRIMER_RIGHT_30_SELF_END_TH=0.00 PRIMER_LEFT_30_HAIRPIN_TH=0.00 PRIMER_RIGHT_30_HAIRPIN_TH=37.44 PRIMER_INTERNAL_30_HAIRPIN_TH=41.14 PRIMER_LEFT_30_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1) PRIMER_RIGHT_30_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_30_END_STABILITY=4.3000 PRIMER_RIGHT_30_END_STABILITY=3.6000 PRIMER_LEFT_30_TEMPLATE_MISPRIMING_TH=5.4315 PRIMER_RIGHT_30_TEMPLATE_MISPRIMING_TH=7.1743 PRIMER_LEFT_31_PENALTY=4.473143 PRIMER_RIGHT_31_PENALTY=4.473143 PRIMER_INTERNAL_31_PENALTY=5.152054 PRIMER_LEFT_31_SEQUENCE=TTCCAAAACGCAAAGCACGCT PRIMER_RIGHT_31_SEQUENCE=AGCGTGCTTTGCGTTTTGGAA PRIMER_INTERNAL_31_SEQUENCE=TTCCAAAACGCAAAGCACGCTCCC PRIMER_LEFT_31=119,21 PRIMER_RIGHT_31=139,21 PRIMER_INTERNAL_31=119,24 PRIMER_LEFT_31_TM=62.473 PRIMER_RIGHT_31_TM=62.473 PRIMER_INTERNAL_31_TM=61.152 PRIMER_LEFT_31_GC_PERCENT=47.619 PRIMER_RIGHT_31_GC_PERCENT=47.619 PRIMER_INTERNAL_31_GC_PERCENT=54.167 PRIMER_INTERNAL_31_SELF_ANY_TH=0.00 PRIMER_LEFT_31_SELF_ANY_TH=0.00 PRIMER_RIGHT_31_SELF_ANY_TH=0.00 PRIMER_INTERNAL_31_SELF_END_TH=0.00 PRIMER_LEFT_31_SELF_END_TH=0.00 PRIMER_RIGHT_31_SELF_END_TH=0.00 PRIMER_LEFT_31_HAIRPIN_TH=0.00 PRIMER_RIGHT_31_HAIRPIN_TH=37.44 PRIMER_INTERNAL_31_HAIRPIN_TH=0.00 PRIMER_LEFT_31_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_31_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_31_END_STABILITY=5.0700 PRIMER_RIGHT_31_END_STABILITY=3.5300 PRIMER_LEFT_31_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_31_TEMPLATE_MISPRIMING_TH=9.3017 PRIMER_LEFT_32_PENALTY=4.837196 PRIMER_RIGHT_32_PENALTY=4.837196 PRIMER_INTERNAL_32_PENALTY=5.256814 PRIMER_LEFT_32_SEQUENCE=TCCAAAACGCAAAGCACGCTC PRIMER_RIGHT_32_SEQUENCE=GAGCGTGCTTTGCGTTTTGGA PRIMER_INTERNAL_32_SEQUENCE=CAAAACGCAAAGCACGCTCCCGAT PRIMER_LEFT_32=120,21 PRIMER_RIGHT_32=140,21 PRIMER_INTERNAL_32=122,24 PRIMER_LEFT_32_TM=62.837 PRIMER_RIGHT_32_TM=62.837 PRIMER_INTERNAL_32_TM=61.257 PRIMER_LEFT_32_GC_PERCENT=52.381 PRIMER_RIGHT_32_GC_PERCENT=52.381 PRIMER_INTERNAL_32_GC_PERCENT=54.167 PRIMER_INTERNAL_32_SELF_ANY_TH=7.82 PRIMER_LEFT_32_SELF_ANY_TH=0.00 PRIMER_RIGHT_32_SELF_ANY_TH=0.00 PRIMER_INTERNAL_32_SELF_END_TH=0.00 PRIMER_LEFT_32_SELF_END_TH=0.00 PRIMER_RIGHT_32_SELF_END_TH=0.00 PRIMER_LEFT_32_HAIRPIN_TH=0.00 PRIMER_RIGHT_32_HAIRPIN_TH=37.44 PRIMER_INTERNAL_32_HAIRPIN_TH=36.42 PRIMER_LEFT_32_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_RIGHT_32_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_32_END_STABILITY=5.0300 PRIMER_RIGHT_32_END_STABILITY=3.5300 PRIMER_LEFT_32_TEMPLATE_MISPRIMING_TH=8.9959 PRIMER_RIGHT_32_TEMPLATE_MISPRIMING_TH=4.7683 PRIMER_INTERNAL_33_PENALTY=5.325970 PRIMER_INTERNAL_33_SEQUENCE=ATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_33=118,23 PRIMER_INTERNAL_33_TM=57.674 PRIMER_INTERNAL_33_GC_PERCENT=47.826 PRIMER_INTERNAL_33_SELF_ANY_TH=0.00 PRIMER_INTERNAL_33_SELF_END_TH=0.00 PRIMER_INTERNAL_33_HAIRPIN_TH=0.00 PRIMER_INTERNAL_34_PENALTY=5.931698 PRIMER_INTERNAL_34_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCC PRIMER_INTERNAL_34=117,25 PRIMER_INTERNAL_34_TM=59.068 PRIMER_INTERNAL_34_GC_PERCENT=48.000 PRIMER_INTERNAL_34_SELF_ANY_TH=0.00 PRIMER_INTERNAL_34_SELF_END_TH=0.00 PRIMER_INTERNAL_34_HAIRPIN_TH=0.00 PRIMER_INTERNAL_35_PENALTY=6.171494 PRIMER_INTERNAL_35_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCC PRIMER_INTERNAL_35=118,25 PRIMER_INTERNAL_35_TM=61.171 PRIMER_INTERNAL_35_GC_PERCENT=52.000 PRIMER_INTERNAL_35_SELF_ANY_TH=0.00 PRIMER_INTERNAL_35_SELF_END_TH=0.00 PRIMER_INTERNAL_35_HAIRPIN_TH=0.00 PRIMER_INTERNAL_36_PENALTY=6.354311 PRIMER_INTERNAL_36_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCC PRIMER_INTERNAL_36=116,26 PRIMER_INTERNAL_36_TM=59.646 PRIMER_INTERNAL_36_GC_PERCENT=50.000 PRIMER_INTERNAL_36_SELF_ANY_TH=0.00 PRIMER_INTERNAL_36_SELF_END_TH=0.00 PRIMER_INTERNAL_36_HAIRPIN_TH=0.00 PRIMER_INTERNAL_37_PENALTY=6.689016 PRIMER_INTERNAL_37_SEQUENCE=TATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_37=117,24 PRIMER_INTERNAL_37_TM=57.311 PRIMER_INTERNAL_37_GC_PERCENT=45.833 PRIMER_INTERNAL_37_SELF_ANY_TH=0.00 PRIMER_INTERNAL_37_SELF_END_TH=0.00 PRIMER_INTERNAL_37_HAIRPIN_TH=0.00 PRIMER_INTERNAL_38_PENALTY=6.689016 PRIMER_INTERNAL_38_SEQUENCE=TCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_38=115,24 PRIMER_INTERNAL_38_TM=57.311 PRIMER_INTERNAL_38_GC_PERCENT=45.833 PRIMER_INTERNAL_38_SELF_ANY_TH=0.00 PRIMER_INTERNAL_38_SELF_END_TH=0.00 PRIMER_INTERNAL_38_HAIRPIN_TH=0.00 PRIMER_INTERNAL_39_PENALTY=6.694952 PRIMER_INTERNAL_39_SEQUENCE=CTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_39=116,24 PRIMER_INTERNAL_39_TM=57.305 PRIMER_INTERNAL_39_GC_PERCENT=45.833 PRIMER_INTERNAL_39_SELF_ANY_TH=0.00 PRIMER_INTERNAL_39_SELF_END_TH=0.00 PRIMER_INTERNAL_39_HAIRPIN_TH=0.00 PRIMER_INTERNAL_40_PENALTY=6.707310 PRIMER_INTERNAL_40_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCC PRIMER_INTERNAL_40=117,26 PRIMER_INTERNAL_40_TM=60.707 PRIMER_INTERNAL_40_GC_PERCENT=50.000 PRIMER_INTERNAL_40_SELF_ANY_TH=0.00 PRIMER_INTERNAL_40_SELF_END_TH=0.00 PRIMER_INTERNAL_40_HAIRPIN_TH=0.00 PRIMER_INTERNAL_41_PENALTY=6.724062 PRIMER_INTERNAL_41_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_41=115,25 PRIMER_INTERNAL_41_TM=58.276 PRIMER_INTERNAL_41_GC_PERCENT=44.000 PRIMER_INTERNAL_41_SELF_ANY_TH=0.00 PRIMER_INTERNAL_41_SELF_END_TH=0.00 PRIMER_INTERNAL_41_HAIRPIN_TH=0.00 PRIMER_INTERNAL_42_PENALTY=6.744217 PRIMER_INTERNAL_42_SEQUENCE=CAAAACGCAAAGCACGCTCCCGATC PRIMER_INTERNAL_42=122,25 PRIMER_INTERNAL_42_TM=61.744 PRIMER_INTERNAL_42_GC_PERCENT=56.000 PRIMER_INTERNAL_42_SELF_ANY_TH=0.00 PRIMER_INTERNAL_42_SELF_END_TH=0.00 PRIMER_INTERNAL_42_HAIRPIN_TH=36.42 PRIMER_INTERNAL_43_PENALTY=6.785590 PRIMER_INTERNAL_43_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_43=114,25 PRIMER_INTERNAL_43_TM=58.214 PRIMER_INTERNAL_43_GC_PERCENT=48.000 PRIMER_INTERNAL_43_SELF_ANY_TH=0.00 PRIMER_INTERNAL_43_SELF_END_TH=0.00 PRIMER_INTERNAL_43_HAIRPIN_TH=0.00 PRIMER_INTERNAL_44_PENALTY=6.889273 PRIMER_INTERNAL_44_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_44=114,26 PRIMER_INTERNAL_44_TM=59.111 PRIMER_INTERNAL_44_GC_PERCENT=46.154 PRIMER_INTERNAL_44_SELF_ANY_TH=0.00 PRIMER_INTERNAL_44_SELF_END_TH=0.00 PRIMER_INTERNAL_44_HAIRPIN_TH=0.00 PRIMER_INTERNAL_45_PENALTY=6.889273 PRIMER_INTERNAL_45_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_45=113,26 PRIMER_INTERNAL_45_TM=59.111 PRIMER_INTERNAL_45_GC_PERCENT=46.154 PRIMER_INTERNAL_45_SELF_ANY_TH=0.00 PRIMER_INTERNAL_45_SELF_END_TH=0.00 PRIMER_INTERNAL_45_HAIRPIN_TH=0.00 PRIMER_INTERNAL_46_PENALTY=6.910113 PRIMER_INTERNAL_46_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGA PRIMER_INTERNAL_46=121,24 PRIMER_INTERNAL_46_TM=62.910 PRIMER_INTERNAL_46_GC_PERCENT=58.333 PRIMER_INTERNAL_46_SELF_ANY_TH=7.82 PRIMER_INTERNAL_46_SELF_END_TH=0.00 PRIMER_INTERNAL_46_HAIRPIN_TH=36.42 PRIMER_INTERNAL_47_PENALTY=6.910113 PRIMER_INTERNAL_47_SEQUENCE=TCCAAAACGCAAAGCACGCTCCCG PRIMER_INTERNAL_47=120,24 PRIMER_INTERNAL_47_TM=62.910 PRIMER_INTERNAL_47_GC_PERCENT=58.333 PRIMER_INTERNAL_47_SELF_ANY_TH=1.50 PRIMER_INTERNAL_47_SELF_END_TH=1.50 PRIMER_INTERNAL_47_HAIRPIN_TH=41.14 PRIMER_INTERNAL_48_PENALTY=7.008353 PRIMER_INTERNAL_48_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_48=116,25 PRIMER_INTERNAL_48_TM=57.992 PRIMER_INTERNAL_48_GC_PERCENT=48.000 PRIMER_INTERNAL_48_SELF_ANY_TH=0.00 PRIMER_INTERNAL_48_SELF_END_TH=0.00 PRIMER_INTERNAL_48_HAIRPIN_TH=0.00 PRIMER_INTERNAL_49_PENALTY=7.035358 PRIMER_INTERNAL_49_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_49=113,27 PRIMER_INTERNAL_49_TM=59.965 PRIMER_INTERNAL_49_GC_PERCENT=44.444 PRIMER_INTERNAL_49_SELF_ANY_TH=0.00 PRIMER_INTERNAL_49_SELF_END_TH=0.00 PRIMER_INTERNAL_49_HAIRPIN_TH=0.00 PRIMER_INTERNAL_50_PENALTY=7.102160 PRIMER_INTERNAL_50_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_50=115,26 PRIMER_INTERNAL_50_TM=58.898 PRIMER_INTERNAL_50_GC_PERCENT=46.154 PRIMER_INTERNAL_50_SELF_ANY_TH=0.00 PRIMER_INTERNAL_50_SELF_END_TH=0.00 PRIMER_INTERNAL_50_HAIRPIN_TH=0.00 PRIMER_INTERNAL_51_PENALTY=7.334450 PRIMER_INTERNAL_51_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_51=114,27 PRIMER_INTERNAL_51_TM=59.666 PRIMER_INTERNAL_51_GC_PERCENT=48.148 PRIMER_INTERNAL_51_SELF_ANY_TH=0.00 PRIMER_INTERNAL_51_SELF_END_TH=0.00 PRIMER_INTERNAL_51_HAIRPIN_TH=0.00 PRIMER_INTERNAL_52_PENALTY=7.480679 PRIMER_INTERNAL_52_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTCC PRIMER_INTERNAL_52=115,27 PRIMER_INTERNAL_52_TM=60.481 PRIMER_INTERNAL_52_GC_PERCENT=48.148 PRIMER_INTERNAL_52_SELF_ANY_TH=0.00 PRIMER_INTERNAL_52_SELF_END_TH=0.00 PRIMER_INTERNAL_52_HAIRPIN_TH=0.00 PRIMER_INTERNAL_53_PENALTY=7.647244 PRIMER_INTERNAL_53_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_53=112,27 PRIMER_INTERNAL_53_TM=59.353 PRIMER_INTERNAL_53_GC_PERCENT=44.444 PRIMER_INTERNAL_53_SELF_ANY_TH=0.00 PRIMER_INTERNAL_53_SELF_END_TH=0.00 PRIMER_INTERNAL_53_HAIRPIN_TH=0.00 PRIMER_INTERNAL_54_PENALTY=7.744113 PRIMER_INTERNAL_54_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGT PRIMER_INTERNAL_54=55,26 PRIMER_INTERNAL_54_TM=58.256 PRIMER_INTERNAL_54_GC_PERCENT=46.154 PRIMER_INTERNAL_54_SELF_ANY_TH=0.00 PRIMER_INTERNAL_54_SELF_END_TH=0.00 PRIMER_INTERNAL_54_HAIRPIN_TH=0.00 PRIMER_INTERNAL_55_PENALTY=7.867066 PRIMER_INTERNAL_55_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGAT PRIMER_INTERNAL_55=121,25 PRIMER_INTERNAL_55_TM=62.867 PRIMER_INTERNAL_55_GC_PERCENT=56.000 PRIMER_INTERNAL_55_SELF_ANY_TH=7.82 PRIMER_INTERNAL_55_SELF_END_TH=0.00 PRIMER_INTERNAL_55_HAIRPIN_TH=36.42 PRIMER_INTERNAL_56_PENALTY=7.916609 PRIMER_INTERNAL_56_SEQUENCE=TGACCTGATAGAAACGCCAACTCAC PRIMER_INTERNAL_56=53,25 PRIMER_INTERNAL_56_TM=57.083 PRIMER_INTERNAL_56_GC_PERCENT=48.000 PRIMER_INTERNAL_56_SELF_ANY_TH=0.00 PRIMER_INTERNAL_56_SELF_END_TH=0.00 PRIMER_INTERNAL_56_HAIRPIN_TH=0.00 PRIMER_INTERNAL_57_PENALTY=7.922893 PRIMER_INTERNAL_57_SEQUENCE=CCTGATAGAAACGCCAACTCACTGT PRIMER_INTERNAL_57=56,25 PRIMER_INTERNAL_57_TM=57.077 PRIMER_INTERNAL_57_GC_PERCENT=48.000 PRIMER_INTERNAL_57_SELF_ANY_TH=0.00 PRIMER_INTERNAL_57_SELF_END_TH=0.00 PRIMER_INTERNAL_57_HAIRPIN_TH=0.00 PRIMER_INTERNAL_58_PENALTY=7.922893 PRIMER_INTERNAL_58_SEQUENCE=ACCTGATAGAAACGCCAACTCACTG PRIMER_INTERNAL_58=55,25 PRIMER_INTERNAL_58_TM=57.077 PRIMER_INTERNAL_58_GC_PERCENT=48.000 PRIMER_INTERNAL_58_SELF_ANY_TH=0.00 PRIMER_INTERNAL_58_SELF_END_TH=0.00 PRIMER_INTERNAL_58_HAIRPIN_TH=0.00 PRIMER_INTERNAL_59_PENALTY=7.963894 PRIMER_INTERNAL_59_SEQUENCE=TGACCTGATAGAAACGCCAACTCACT PRIMER_INTERNAL_59=53,26 PRIMER_INTERNAL_59_TM=58.036 PRIMER_INTERNAL_59_GC_PERCENT=46.154 PRIMER_INTERNAL_59_SELF_ANY_TH=0.00 PRIMER_INTERNAL_59_SELF_END_TH=0.00 PRIMER_INTERNAL_59_HAIRPIN_TH=0.00 PRIMER_INTERNAL_60_PENALTY=8.131843 PRIMER_INTERNAL_60_SEQUENCE=TGACCTGATAGAAACGCCAACTCACTG PRIMER_INTERNAL_60=53,27 PRIMER_INTERNAL_60_TM=58.868 PRIMER_INTERNAL_60_GC_PERCENT=48.148 PRIMER_INTERNAL_60_SELF_ANY_TH=0.00 PRIMER_INTERNAL_60_SELF_END_TH=0.00 PRIMER_INTERNAL_60_HAIRPIN_TH=0.00 PRIMER_INTERNAL_61_PENALTY=8.132460 PRIMER_INTERNAL_61_SEQUENCE=GACCTGATAGAAACGCCAACTCACTGT PRIMER_INTERNAL_61=54,27 PRIMER_INTERNAL_61_TM=58.868 PRIMER_INTERNAL_61_GC_PERCENT=48.148 PRIMER_INTERNAL_61_SELF_ANY_TH=0.00 PRIMER_INTERNAL_61_SELF_END_TH=0.00 PRIMER_INTERNAL_61_HAIRPIN_TH=0.00 PRIMER_INTERNAL_62_PENALTY=8.193320 PRIMER_INTERNAL_62_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCCC PRIMER_INTERNAL_62=116,27 PRIMER_INTERNAL_62_TM=61.193 PRIMER_INTERNAL_62_GC_PERCENT=51.852 PRIMER_INTERNAL_62_SELF_ANY_TH=0.00 PRIMER_INTERNAL_62_SELF_END_TH=0.00 PRIMER_INTERNAL_62_HAIRPIN_TH=0.00 PRIMER_INTERNAL_63_PENALTY=8.237701 PRIMER_INTERNAL_63_SEQUENCE=GACCTGATAGAAACGCCAACTCACTG PRIMER_INTERNAL_63=54,26 PRIMER_INTERNAL_63_TM=57.762 PRIMER_INTERNAL_63_GC_PERCENT=50.000 PRIMER_INTERNAL_63_SELF_ANY_TH=0.00 PRIMER_INTERNAL_63_SELF_END_TH=0.00 PRIMER_INTERNAL_63_HAIRPIN_TH=0.00 PRIMER_INTERNAL_64_PENALTY=8.755267 PRIMER_INTERNAL_64_SEQUENCE=ATGACCTGATAGAAACGCCAACTCAC PRIMER_INTERNAL_64=52,26 PRIMER_INTERNAL_64_TM=57.245 PRIMER_INTERNAL_64_GC_PERCENT=46.154 PRIMER_INTERNAL_64_SELF_ANY_TH=0.00 PRIMER_INTERNAL_64_SELF_END_TH=0.00 PRIMER_INTERNAL_64_HAIRPIN_TH=0.00 PRIMER_INTERNAL_65_PENALTY=8.758760 PRIMER_INTERNAL_65_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACG PRIMER_INTERNAL_65=112,26 PRIMER_INTERNAL_65_TM=57.241 PRIMER_INTERNAL_65_GC_PERCENT=42.308 PRIMER_INTERNAL_65_SELF_ANY_TH=0.00 PRIMER_INTERNAL_65_SELF_END_TH=0.00 PRIMER_INTERNAL_65_HAIRPIN_TH=0.00 PRIMER_INTERNAL_66_PENALTY=8.838967 PRIMER_INTERNAL_66_SEQUENCE=ATGACCTGATAGAAACGCCAACTCACT PRIMER_INTERNAL_66=52,27 PRIMER_INTERNAL_66_TM=58.161 PRIMER_INTERNAL_66_GC_PERCENT=44.444 PRIMER_INTERNAL_66_SELF_ANY_TH=0.00 PRIMER_INTERNAL_66_SELF_END_TH=0.00 PRIMER_INTERNAL_66_HAIRPIN_TH=0.00 PRIMER_INTERNAL_67_PENALTY=8.978186 PRIMER_INTERNAL_67_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCCG PRIMER_INTERNAL_67=118,26 PRIMER_INTERNAL_67_TM=62.978 PRIMER_INTERNAL_67_GC_PERCENT=53.846 PRIMER_INTERNAL_67_SELF_ANY_TH=1.50 PRIMER_INTERNAL_67_SELF_END_TH=1.50 PRIMER_INTERNAL_67_HAIRPIN_TH=41.14 PRIMER_INTERNAL_68_PENALTY=9.099564 PRIMER_INTERNAL_68_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGTA PRIMER_INTERNAL_68=55,27 PRIMER_INTERNAL_68_TM=57.900 PRIMER_INTERNAL_68_GC_PERCENT=44.444 PRIMER_INTERNAL_68_SELF_ANY_TH=0.00 PRIMER_INTERNAL_68_SELF_END_TH=0.00 PRIMER_INTERNAL_68_HAIRPIN_TH=0.00 PRIMER_INTERNAL_69_PENALTY=9.448747 PRIMER_INTERNAL_69_SEQUENCE=AAAGTCTATTCCAAAACGCAAAGCACG PRIMER_INTERNAL_69=111,27 PRIMER_INTERNAL_69_TM=57.551 PRIMER_INTERNAL_69_GC_PERCENT=40.741 PRIMER_INTERNAL_69_SELF_ANY_TH=0.00 PRIMER_INTERNAL_69_SELF_END_TH=0.00 PRIMER_INTERNAL_69_HAIRPIN_TH=0.00 PRIMER_INTERNAL_70_PENALTY=9.472089 PRIMER_INTERNAL_70_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCCG PRIMER_INTERNAL_70=117,27 PRIMER_INTERNAL_70_TM=62.472 PRIMER_INTERNAL_70_GC_PERCENT=51.852 PRIMER_INTERNAL_70_SELF_ANY_TH=1.50 PRIMER_INTERNAL_70_SELF_END_TH=1.50 PRIMER_INTERNAL_70_HAIRPIN_TH=41.14 PRIMER_INTERNAL_71_PENALTY=9.912698 PRIMER_INTERNAL_71_SEQUENCE=CGTTGTCAAAGTCTATTCCAAAACGCA PRIMER_INTERNAL_71=104,27 PRIMER_INTERNAL_71_TM=57.087 PRIMER_INTERNAL_71_GC_PERCENT=40.741 PRIMER_INTERNAL_71_SELF_ANY_TH=0.00 PRIMER_INTERNAL_71_SELF_END_TH=0.00 PRIMER_INTERNAL_71_HAIRPIN_TH=0.00 PRIMER_INTERNAL_72_PENALTY=9.914131 PRIMER_INTERNAL_72_SEQUENCE=ACGTTGTCAAAGTCTATTCCAAAACGC PRIMER_INTERNAL_72=103,27 PRIMER_INTERNAL_72_TM=57.086 PRIMER_INTERNAL_72_GC_PERCENT=40.741 PRIMER_INTERNAL_72_SELF_ANY_TH=0.00 PRIMER_INTERNAL_72_SELF_END_TH=0.00 PRIMER_INTERNAL_72_HAIRPIN_TH=0.00 = PRIMER_TASK=pick_primer_list PRIMER_PRODUCT_SIZE_RANGE=75-200 P3_FILE_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=4 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=4 SEQUENCE_ID=pick_primer_list_and_file_flag_th_3 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_LEFT_EXPLAIN=considered 175, too many Ns 19, overlap target 25, low tm 64, high tm 12, high repeat similarity 3, ok 52 PRIMER_RIGHT_EXPLAIN=considered 174, too many Ns 21, overlap target 23, low tm 64, high tm 12, high hairpin stability 16, high repeat similarity 3, ok 35 PRIMER_INTERNAL_EXPLAIN=considered 647, too many Ns 27, low tm 410, high tm 13, ok 197 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=52 PRIMER_RIGHT_NUM_RETURNED=35 PRIMER_INTERNAL_NUM_RETURNED=197 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.527529 PRIMER_RIGHT_0_PENALTY=0.527529 PRIMER_INTERNAL_0_PENALTY=0.727038 PRIMER_LEFT_0_SEQUENCE=TTAGTGGGCCATTCGATCCC PRIMER_RIGHT_0_SEQUENCE=GGGATCGAATGGCCCACTAA PRIMER_INTERNAL_0_SEQUENCE=GGGCCATTCGATCCCGACCC PRIMER_LEFT_0=61,20 PRIMER_RIGHT_0=80,20 PRIMER_INTERNAL_0=66,20 PRIMER_LEFT_0_TM=59.528 PRIMER_RIGHT_0_TM=59.528 PRIMER_INTERNAL_0_TM=59.273 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=70.000 PRIMER_INTERNAL_0_SELF_ANY_TH=2.18 PRIMER_LEFT_0_SELF_ANY_TH=5.82 PRIMER_RIGHT_0_SELF_ANY_TH=10.16 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=5.82 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=32.36 PRIMER_INTERNAL_0_HAIRPIN_TH=41.60 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=2.2400 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=2.6459 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0266 PRIMER_LEFT_1_PENALTY=0.606923 PRIMER_RIGHT_1_PENALTY=0.606923 PRIMER_INTERNAL_1_PENALTY=1.416589 PRIMER_LEFT_1_SEQUENCE=CCCGACCCAAATCGATAGCT PRIMER_RIGHT_1_SEQUENCE=AGCTATCGATTTGGGTCGGG PRIMER_INTERNAL_1_SEQUENCE=GTGGGCCATTCGATCCCGACC PRIMER_LEFT_1=78,20 PRIMER_RIGHT_1=97,20 PRIMER_INTERNAL_1=64,21 PRIMER_LEFT_1_TM=59.607 PRIMER_RIGHT_1_TM=59.607 PRIMER_INTERNAL_1_TM=59.583 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=66.667 PRIMER_INTERNAL_1_SELF_ANY_TH=2.53 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=45.13 PRIMER_RIGHT_1_HAIRPIN_TH=41.74 PRIMER_INTERNAL_1_HAIRPIN_TH=41.60 PRIMER_LEFT_1_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence PRIMER_RIGHT_1_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_LEFT_1_END_STABILITY=3.3200 PRIMER_RIGHT_1_END_STABILITY=5.1400 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=0.0186 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=7.7569 PRIMER_LEFT_2_PENALTY=0.608403 PRIMER_RIGHT_2_PENALTY=0.608403 PRIMER_INTERNAL_2_PENALTY=1.438741 PRIMER_LEFT_2_SEQUENCE=TCCCGACCCAAATCGATAGC PRIMER_RIGHT_2_SEQUENCE=GCTATCGATTTGGGTCGGGA PRIMER_INTERNAL_2_SEQUENCE=GGCCATTCGATCCCGACCCA PRIMER_LEFT_2=77,20 PRIMER_RIGHT_2=96,20 PRIMER_INTERNAL_2=67,20 PRIMER_LEFT_2_TM=59.608 PRIMER_RIGHT_2_TM=59.608 PRIMER_INTERNAL_2_TM=58.561 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=65.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=1.64 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=45.13 PRIMER_RIGHT_2_HAIRPIN_TH=41.74 PRIMER_INTERNAL_2_HAIRPIN_TH=41.60 PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_2_END_STABILITY=2.9700 PRIMER_RIGHT_2_END_STABILITY=5.1400 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=5.2033 PRIMER_LEFT_3_PENALTY=0.713597 PRIMER_RIGHT_3_PENALTY=0.713597 PRIMER_INTERNAL_3_PENALTY=1.438741 PRIMER_LEFT_3_SEQUENCE=GACATGACACTGACGATGCA PRIMER_RIGHT_3_SEQUENCE=TGCATCGTCAGTGTCATGTC PRIMER_INTERNAL_3_SEQUENCE=TGGGCCATTCGATCCCGACC PRIMER_LEFT_3=11,20 PRIMER_RIGHT_3=30,20 PRIMER_INTERNAL_3=65,20 PRIMER_LEFT_3_TM=58.286 PRIMER_RIGHT_3_TM=58.286 PRIMER_INTERNAL_3_TM=58.561 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_SELF_ANY_TH=4.28 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=41.60 PRIMER_LEFT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_3_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_3_END_STABILITY=3.9600 PRIMER_RIGHT_3_END_STABILITY=3.0600 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=4.9455 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.9996 PRIMER_LEFT_4_PENALTY=0.966496 PRIMER_RIGHT_4_PENALTY=0.966496 PRIMER_INTERNAL_4_PENALTY=1.650306 PRIMER_LEFT_4_SEQUENCE=CGATCCCGACCCAAATCGAT PRIMER_RIGHT_4_SEQUENCE=ATCGATTTGGGTCGGGATCG PRIMER_INTERNAL_4_SEQUENCE=GGGCCATTCGATCCCGACCCA PRIMER_LEFT_4=74,20 PRIMER_RIGHT_4=93,20 PRIMER_INTERNAL_4=66,21 PRIMER_LEFT_4_TM=59.966 PRIMER_RIGHT_4_TM=59.966 PRIMER_INTERNAL_4_TM=60.650 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=66.667 PRIMER_INTERNAL_4_SELF_ANY_TH=2.16 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=45.13 PRIMER_RIGHT_4_HAIRPIN_TH=41.74 PRIMER_INTERNAL_4_HAIRPIN_TH=41.60 PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_4_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_4_END_STABILITY=3.5900 PRIMER_RIGHT_4_END_STABILITY=3.6900 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=2.7399 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=6.2638 PRIMER_LEFT_5_PENALTY=0.967319 PRIMER_RIGHT_5_PENALTY=1.001812 PRIMER_INTERNAL_5_PENALTY=1.650306 PRIMER_LEFT_5_SEQUENCE=CATTCGATCCCGACCCAAAT PRIMER_RIGHT_5_SEQUENCE=GTCTGCATCGTCAGTGTCAT PRIMER_INTERNAL_5_SEQUENCE=TGGGCCATTCGATCCCGACCC PRIMER_LEFT_5=70,20 PRIMER_RIGHT_5=33,20 PRIMER_INTERNAL_5=65,21 PRIMER_LEFT_5_TM=58.033 PRIMER_RIGHT_5_TM=57.998 PRIMER_INTERNAL_5_TM=60.650 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=50.000 PRIMER_INTERNAL_5_GC_PERCENT=66.667 PRIMER_INTERNAL_5_SELF_ANY_TH=4.28 PRIMER_LEFT_5_SELF_ANY_TH=0.00 PRIMER_RIGHT_5_SELF_ANY_TH=0.00 PRIMER_INTERNAL_5_SELF_END_TH=0.51 PRIMER_LEFT_5_SELF_END_TH=0.00 PRIMER_RIGHT_5_SELF_END_TH=0.00 PRIMER_LEFT_5_HAIRPIN_TH=45.50 PRIMER_RIGHT_5_HAIRPIN_TH=0.00 PRIMER_INTERNAL_5_HAIRPIN_TH=41.60 PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_5_END_STABILITY=2.3200 PRIMER_RIGHT_5_END_STABILITY=3.0600 PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=2.1397 PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_6_PENALTY=1.000429 PRIMER_RIGHT_6_PENALTY=1.001812 PRIMER_INTERNAL_6_PENALTY=2.095978 PRIMER_LEFT_6_SEQUENCE=CATGACACTGACGATGCAGA PRIMER_RIGHT_6_SEQUENCE=GCATCGTCAGTGTCATGTCT PRIMER_INTERNAL_6_SEQUENCE=GGCCATTCGATCCCGACCCAA PRIMER_LEFT_6=13,20 PRIMER_RIGHT_6=29,20 PRIMER_INTERNAL_6=67,21 PRIMER_LEFT_6_TM=58.000 PRIMER_RIGHT_6_TM=57.998 PRIMER_INTERNAL_6_TM=58.904 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_INTERNAL_6_GC_PERCENT=61.905 PRIMER_INTERNAL_6_SELF_ANY_TH=0.00 PRIMER_LEFT_6_SELF_ANY_TH=10.65 PRIMER_RIGHT_6_SELF_ANY_TH=0.00 PRIMER_INTERNAL_6_SELF_END_TH=0.00 PRIMER_LEFT_6_SELF_END_TH=0.00 PRIMER_RIGHT_6_SELF_END_TH=0.00 PRIMER_LEFT_6_HAIRPIN_TH=0.00 PRIMER_RIGHT_6_HAIRPIN_TH=0.00 PRIMER_INTERNAL_6_HAIRPIN_TH=41.60 PRIMER_LEFT_6_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_6_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_6_END_STABILITY=4.2600 PRIMER_RIGHT_6_END_STABILITY=3.4100 PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=1.1151 PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=15.2988 PRIMER_LEFT_7_PENALTY=1.001812 PRIMER_RIGHT_7_PENALTY=1.142220 PRIMER_INTERNAL_7_PENALTY=2.360266 PRIMER_LEFT_7_SEQUENCE=ATGACACTGACGATGCAGAC PRIMER_RIGHT_7_SEQUENCE=GGGATCGAATGGCCCACTA PRIMER_INTERNAL_7_SEQUENCE=AGTGGGCCATTCGATCCCGAC PRIMER_LEFT_7=14,20 PRIMER_RIGHT_7=80,19 PRIMER_INTERNAL_7=63,21 PRIMER_LEFT_7_TM=57.998 PRIMER_RIGHT_7_TM=58.858 PRIMER_INTERNAL_7_TM=58.640 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=57.895 PRIMER_INTERNAL_7_GC_PERCENT=61.905 PRIMER_INTERNAL_7_SELF_ANY_TH=3.57 PRIMER_LEFT_7_SELF_ANY_TH=4.19 PRIMER_RIGHT_7_SELF_ANY_TH=10.16 PRIMER_INTERNAL_7_SELF_END_TH=3.02 PRIMER_LEFT_7_SELF_END_TH=0.00 PRIMER_RIGHT_7_SELF_END_TH=0.00 PRIMER_LEFT_7_HAIRPIN_TH=0.00 PRIMER_RIGHT_7_HAIRPIN_TH=32.36 PRIMER_INTERNAL_7_HAIRPIN_TH=41.60 PRIMER_LEFT_7_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_7_END_STABILITY=3.5100 PRIMER_RIGHT_7_END_STABILITY=2.7400 PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=8.4593 PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=7.9828 PRIMER_LEFT_8_PENALTY=1.001812 PRIMER_RIGHT_8_PENALTY=1.316792 PRIMER_INTERNAL_8_PENALTY=2.456420 PRIMER_LEFT_8_SEQUENCE=AGACATGACACTGACGATGC PRIMER_RIGHT_8_SEQUENCE=CGTAGCTATCGATTTGGGTCG PRIMER_INTERNAL_8_SEQUENCE=AGTGGGCCATTCGATCCCGA PRIMER_LEFT_8=10,20 PRIMER_RIGHT_8=100,21 PRIMER_INTERNAL_8=63,20 PRIMER_LEFT_8_TM=57.998 PRIMER_RIGHT_8_TM=58.683 PRIMER_INTERNAL_8_TM=57.544 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=52.381 PRIMER_INTERNAL_8_GC_PERCENT=60.000 PRIMER_INTERNAL_8_SELF_ANY_TH=3.28 PRIMER_LEFT_8_SELF_ANY_TH=0.00 PRIMER_RIGHT_8_SELF_ANY_TH=9.28 PRIMER_INTERNAL_8_SELF_END_TH=0.00 PRIMER_LEFT_8_SELF_END_TH=0.00 PRIMER_RIGHT_8_SELF_END_TH=4.23 PRIMER_LEFT_8_HAIRPIN_TH=0.00 PRIMER_RIGHT_8_HAIRPIN_TH=42.74 PRIMER_INTERNAL_8_HAIRPIN_TH=41.96 PRIMER_LEFT_8_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, MER4A a consensus PRIMER_LEFT_8_END_STABILITY=3.9100 PRIMER_RIGHT_8_END_STABILITY=4.7900 PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=8.8342 PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=11.4930 PRIMER_LEFT_9_PENALTY=1.107410 PRIMER_RIGHT_9_PENALTY=1.383135 PRIMER_INTERNAL_9_PENALTY=2.511372 PRIMER_LEFT_9_SEQUENCE=CCATTCGATCCCGACCCAAA PRIMER_RIGHT_9_SEQUENCE=TAGCTATCGATTTGGGTCGGG PRIMER_INTERNAL_9_SEQUENCE=GTGGGCCATTCGATCCCGAC PRIMER_LEFT_9=69,20 PRIMER_RIGHT_9=98,21 PRIMER_INTERNAL_9=64,20 PRIMER_LEFT_9_TM=60.107 PRIMER_RIGHT_9_TM=59.383 PRIMER_INTERNAL_9_TM=57.489 PRIMER_LEFT_9_GC_PERCENT=55.000 PRIMER_RIGHT_9_GC_PERCENT=52.381 PRIMER_INTERNAL_9_GC_PERCENT=65.000 PRIMER_INTERNAL_9_SELF_ANY_TH=0.97 PRIMER_LEFT_9_SELF_ANY_TH=0.00 PRIMER_RIGHT_9_SELF_ANY_TH=0.00 PRIMER_INTERNAL_9_SELF_END_TH=0.00 PRIMER_LEFT_9_SELF_END_TH=0.00 PRIMER_RIGHT_9_SELF_END_TH=0.00 PRIMER_LEFT_9_HAIRPIN_TH=45.50 PRIMER_RIGHT_9_HAIRPIN_TH=41.74 PRIMER_INTERNAL_9_HAIRPIN_TH=41.60 PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_LEFT_9_END_STABILITY=3.2800 PRIMER_RIGHT_9_END_STABILITY=5.1400 PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=7.7569 PRIMER_LEFT_10_PENALTY=1.126409 PRIMER_RIGHT_10_PENALTY=1.559940 PRIMER_INTERNAL_10_PENALTY=2.626619 PRIMER_LEFT_10_SEQUENCE=CATTCGATCCCGACCCAAATC PRIMER_RIGHT_10_SEQUENCE=TCGATTTGGGTCGGGATCG PRIMER_INTERNAL_10_SEQUENCE=AGTGGGCCATTCGATCCCGACC PRIMER_LEFT_10=70,21 PRIMER_RIGHT_10=92,19 PRIMER_INTERNAL_10=63,22 PRIMER_LEFT_10_TM=59.126 PRIMER_RIGHT_10_TM=59.560 PRIMER_INTERNAL_10_TM=60.627 PRIMER_LEFT_10_GC_PERCENT=52.381 PRIMER_RIGHT_10_GC_PERCENT=57.895 PRIMER_INTERNAL_10_GC_PERCENT=63.636 PRIMER_INTERNAL_10_SELF_ANY_TH=5.61 PRIMER_LEFT_10_SELF_ANY_TH=0.00 PRIMER_RIGHT_10_SELF_ANY_TH=0.00 PRIMER_INTERNAL_10_SELF_END_TH=0.00 PRIMER_LEFT_10_SELF_END_TH=0.00 PRIMER_RIGHT_10_SELF_END_TH=0.00 PRIMER_LEFT_10_HAIRPIN_TH=45.50 PRIMER_RIGHT_10_HAIRPIN_TH=41.74 PRIMER_INTERNAL_10_HAIRPIN_TH=41.60 PRIMER_LEFT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_RIGHT_10_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_10_END_STABILITY=2.1700 PRIMER_RIGHT_10_END_STABILITY=3.6900 PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=3.6627 PRIMER_LEFT_11_PENALTY=1.142220 PRIMER_RIGHT_11_PENALTY=1.559940 PRIMER_INTERNAL_11_PENALTY=2.783921 PRIMER_LEFT_11_SEQUENCE=TAGTGGGCCATTCGATCCC PRIMER_RIGHT_11_SEQUENCE=CGATTTGGGTCGGGATCGA PRIMER_INTERNAL_11_SEQUENCE=GGCCATTCGATCCCGACCCAAA PRIMER_LEFT_11=62,19 PRIMER_RIGHT_11=91,19 PRIMER_INTERNAL_11=67,22 PRIMER_LEFT_11_TM=58.858 PRIMER_RIGHT_11_TM=59.560 PRIMER_INTERNAL_11_TM=59.216 PRIMER_LEFT_11_GC_PERCENT=57.895 PRIMER_RIGHT_11_GC_PERCENT=57.895 PRIMER_INTERNAL_11_GC_PERCENT=59.091 PRIMER_INTERNAL_11_SELF_ANY_TH=0.00 PRIMER_LEFT_11_SELF_ANY_TH=5.82 PRIMER_RIGHT_11_SELF_ANY_TH=0.00 PRIMER_INTERNAL_11_SELF_END_TH=0.00 PRIMER_LEFT_11_SELF_END_TH=5.82 PRIMER_RIGHT_11_SELF_END_TH=0.00 PRIMER_LEFT_11_HAIRPIN_TH=0.00 PRIMER_RIGHT_11_HAIRPIN_TH=43.86 PRIMER_INTERNAL_11_HAIRPIN_TH=41.60 PRIMER_LEFT_11_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_11_END_STABILITY=3.8500 PRIMER_RIGHT_11_END_STABILITY=3.5900 PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=7.8904 PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_12_PENALTY=1.179094 PRIMER_RIGHT_12_PENALTY=1.568623 PRIMER_INTERNAL_12_PENALTY=2.885177 PRIMER_LEFT_12_SEQUENCE=TTCGATCCCGACCCAAATCG PRIMER_RIGHT_12_SEQUENCE=GGATCGAATGGCCCACTAAT PRIMER_INTERNAL_12_SEQUENCE=GGGCCATTCGATCCCGACCCAA PRIMER_LEFT_12=72,20 PRIMER_RIGHT_12=79,20 PRIMER_INTERNAL_12=66,22 PRIMER_LEFT_12_TM=60.179 PRIMER_RIGHT_12_TM=57.431 PRIMER_INTERNAL_12_TM=60.885 PRIMER_LEFT_12_GC_PERCENT=55.000 PRIMER_RIGHT_12_GC_PERCENT=50.000 PRIMER_INTERNAL_12_GC_PERCENT=63.636 PRIMER_INTERNAL_12_SELF_ANY_TH=2.16 PRIMER_LEFT_12_SELF_ANY_TH=0.00 PRIMER_RIGHT_12_SELF_ANY_TH=0.00 PRIMER_INTERNAL_12_SELF_END_TH=0.00 PRIMER_LEFT_12_SELF_END_TH=0.00 PRIMER_RIGHT_12_SELF_END_TH=0.00 PRIMER_LEFT_12_HAIRPIN_TH=45.50 PRIMER_RIGHT_12_HAIRPIN_TH=0.00 PRIMER_INTERNAL_12_HAIRPIN_TH=41.60 PRIMER_LEFT_12_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_12_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA PRIMER_LEFT_12_END_STABILITY=3.3400 PRIMER_RIGHT_12_END_STABILITY=1.7300 PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=2.8344 PRIMER_LEFT_13_PENALTY=1.256117 PRIMER_RIGHT_13_PENALTY=1.673833 PRIMER_INTERNAL_13_PENALTY=3.114431 PRIMER_LEFT_13_SEQUENCE=ATTCGATCCCGACCCAAATC PRIMER_RIGHT_13_SEQUENCE=GTAGCTATCGATTTGGGTCGG PRIMER_INTERNAL_13_SEQUENCE=TAGTGGGCCATTCGATCCCGACC PRIMER_LEFT_13=71,20 PRIMER_RIGHT_13=99,21 PRIMER_INTERNAL_13=62,23 PRIMER_LEFT_13_TM=57.744 PRIMER_RIGHT_13_TM=58.326 PRIMER_INTERNAL_13_TM=60.114 PRIMER_LEFT_13_GC_PERCENT=50.000 PRIMER_RIGHT_13_GC_PERCENT=52.381 PRIMER_INTERNAL_13_GC_PERCENT=60.870 PRIMER_INTERNAL_13_SELF_ANY_TH=5.61 PRIMER_LEFT_13_SELF_ANY_TH=0.00 PRIMER_RIGHT_13_SELF_ANY_TH=0.00 PRIMER_INTERNAL_13_SELF_END_TH=0.00 PRIMER_LEFT_13_SELF_END_TH=0.00 PRIMER_RIGHT_13_SELF_END_TH=0.00 PRIMER_LEFT_13_HAIRPIN_TH=45.50 PRIMER_RIGHT_13_HAIRPIN_TH=41.74 PRIMER_INTERNAL_13_HAIRPIN_TH=41.60 PRIMER_LEFT_13_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_RIGHT_13_LIBRARY_MISPRIMING=11.00, MER4A a consensus PRIMER_LEFT_13_END_STABILITY=2.1700 PRIMER_RIGHT_13_END_STABILITY=4.7900 PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=0.0372 PRIMER_LEFT_14_PENALTY=1.316792 PRIMER_RIGHT_14_PENALTY=1.726673 PRIMER_INTERNAL_14_PENALTY=3.510595 PRIMER_LEFT_14_SEQUENCE=CGACCCAAATCGATAGCTACG PRIMER_RIGHT_14_SEQUENCE=TGCATCGTCAGTGTCATGTCT PRIMER_INTERNAL_14_SEQUENCE=GTGGGCCATTCGATCCCGACCC PRIMER_LEFT_14=80,21 PRIMER_RIGHT_14=30,21 PRIMER_INTERNAL_14=64,22 PRIMER_LEFT_14_TM=58.683 PRIMER_RIGHT_14_TM=59.727 PRIMER_INTERNAL_14_TM=61.511 PRIMER_LEFT_14_GC_PERCENT=52.381 PRIMER_RIGHT_14_GC_PERCENT=47.619 PRIMER_INTERNAL_14_GC_PERCENT=68.182 PRIMER_INTERNAL_14_SELF_ANY_TH=2.53 PRIMER_LEFT_14_SELF_ANY_TH=8.77 PRIMER_RIGHT_14_SELF_ANY_TH=1.94 PRIMER_INTERNAL_14_SELF_END_TH=0.25 PRIMER_LEFT_14_SELF_END_TH=8.77 PRIMER_RIGHT_14_SELF_END_TH=0.00 PRIMER_LEFT_14_HAIRPIN_TH=45.13 PRIMER_RIGHT_14_HAIRPIN_TH=0.00 PRIMER_INTERNAL_14_HAIRPIN_TH=41.60 PRIMER_LEFT_14_LIBRARY_MISPRIMING=10.00, MER4A a consensus PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_14_END_STABILITY=3.5100 PRIMER_RIGHT_14_END_STABILITY=3.4100 PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=4.9773 PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=6.6554 PRIMER_LEFT_15_PENALTY=1.383135 PRIMER_RIGHT_15_PENALTY=1.728621 PRIMER_INTERNAL_15_PENALTY=3.685489 PRIMER_LEFT_15_SEQUENCE=CCCGACCCAAATCGATAGCTA PRIMER_RIGHT_15_SEQUENCE=TATCGATTTGGGTCGGGATCG PRIMER_INTERNAL_15_SEQUENCE=GGCCATTCGATCCCGACCCAAAT PRIMER_LEFT_15=78,21 PRIMER_RIGHT_15=94,21 PRIMER_INTERNAL_15=67,23 PRIMER_LEFT_15_TM=59.383 PRIMER_RIGHT_15_TM=59.729 PRIMER_INTERNAL_15_TM=59.315 PRIMER_LEFT_15_GC_PERCENT=52.381 PRIMER_RIGHT_15_GC_PERCENT=52.381 PRIMER_INTERNAL_15_GC_PERCENT=56.522 PRIMER_INTERNAL_15_SELF_ANY_TH=0.00 PRIMER_LEFT_15_SELF_ANY_TH=0.00 PRIMER_RIGHT_15_SELF_ANY_TH=1.95 PRIMER_INTERNAL_15_SELF_END_TH=0.00 PRIMER_LEFT_15_SELF_END_TH=0.00 PRIMER_RIGHT_15_SELF_END_TH=0.00 PRIMER_LEFT_15_HAIRPIN_TH=45.13 PRIMER_RIGHT_15_HAIRPIN_TH=41.74 PRIMER_INTERNAL_15_HAIRPIN_TH=41.60 PRIMER_LEFT_15_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_15_END_STABILITY=3.3200 PRIMER_RIGHT_15_END_STABILITY=3.6900 PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=2.9487 PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=9.4327 PRIMER_LEFT_16_PENALTY=1.475039 PRIMER_RIGHT_16_PENALTY=1.914379 PRIMER_INTERNAL_16_PENALTY=3.807618 PRIMER_LEFT_16_SEQUENCE=CCATTCGATCCCGACCCAA PRIMER_RIGHT_16_SEQUENCE=GCTATCGATTTGGGTCGGG PRIMER_INTERNAL_16_SEQUENCE=TAGTGGGCCATTCGATCCCGAC PRIMER_LEFT_16=69,19 PRIMER_RIGHT_16=96,19 PRIMER_INTERNAL_16=62,22 PRIMER_LEFT_16_TM=59.475 PRIMER_RIGHT_16_TM=58.086 PRIMER_INTERNAL_16_TM=58.192 PRIMER_LEFT_16_GC_PERCENT=57.895 PRIMER_RIGHT_16_GC_PERCENT=57.895 PRIMER_INTERNAL_16_GC_PERCENT=59.091 PRIMER_INTERNAL_16_SELF_ANY_TH=3.57 PRIMER_LEFT_16_SELF_ANY_TH=0.00 PRIMER_RIGHT_16_SELF_ANY_TH=1.64 PRIMER_INTERNAL_16_SELF_END_TH=3.02 PRIMER_LEFT_16_SELF_END_TH=0.00 PRIMER_RIGHT_16_SELF_END_TH=0.00 PRIMER_LEFT_16_HAIRPIN_TH=45.50 PRIMER_RIGHT_16_HAIRPIN_TH=41.74 PRIMER_INTERNAL_16_HAIRPIN_TH=41.60 PRIMER_LEFT_16_LIBRARY_MISPRIMING=9.00, THE1b consensus sequence PRIMER_RIGHT_16_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_LEFT_16_END_STABILITY=4.1200 PRIMER_RIGHT_16_END_STABILITY=5.1400 PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=5.1442 PRIMER_LEFT_17_PENALTY=1.559940 PRIMER_RIGHT_17_PENALTY=1.926077 PRIMER_INTERNAL_17_PENALTY=3.826170 PRIMER_LEFT_17_SEQUENCE=CGATCCCGACCCAAATCGA PRIMER_RIGHT_17_SEQUENCE=GGGATCGAATGGCCCACTAAT PRIMER_INTERNAL_17_SEQUENCE=GCCATTCGATCCCGACCCAAA PRIMER_LEFT_17=74,19 PRIMER_RIGHT_17=80,21 PRIMER_INTERNAL_17=68,21 PRIMER_LEFT_17_TM=59.560 PRIMER_RIGHT_17_TM=59.926 PRIMER_INTERNAL_17_TM=57.174 PRIMER_LEFT_17_GC_PERCENT=57.895 PRIMER_RIGHT_17_GC_PERCENT=52.381 PRIMER_INTERNAL_17_GC_PERCENT=57.143 PRIMER_INTERNAL_17_SELF_ANY_TH=0.00 PRIMER_LEFT_17_SELF_ANY_TH=0.00 PRIMER_RIGHT_17_SELF_ANY_TH=10.16 PRIMER_INTERNAL_17_SELF_END_TH=0.00 PRIMER_LEFT_17_SELF_END_TH=0.00 PRIMER_RIGHT_17_SELF_END_TH=0.00 PRIMER_LEFT_17_HAIRPIN_TH=45.13 PRIMER_RIGHT_17_HAIRPIN_TH=32.36 PRIMER_INTERNAL_17_HAIRPIN_TH=41.60 PRIMER_LEFT_17_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_RIGHT_17_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA PRIMER_LEFT_17_END_STABILITY=3.5900 PRIMER_RIGHT_17_END_STABILITY=1.7300 PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=1.1258 PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=12.3668 PRIMER_LEFT_18_PENALTY=1.559940 PRIMER_RIGHT_18_PENALTY=1.960819 PRIMER_INTERNAL_18_PENALTY=3.864865 PRIMER_LEFT_18_SEQUENCE=TCGATCCCGACCCAAATCG PRIMER_RIGHT_18_SEQUENCE=CTATCGATTTGGGTCGGGATC PRIMER_INTERNAL_18_SEQUENCE=ACACACACACACAGCACACAGGT PRIMER_LEFT_18=73,19 PRIMER_RIGHT_18=95,21 PRIMER_INTERNAL_18=37,23 PRIMER_LEFT_18_TM=59.560 PRIMER_RIGHT_18_TM=58.039 PRIMER_INTERNAL_18_TM=59.135 PRIMER_LEFT_18_GC_PERCENT=57.895 PRIMER_RIGHT_18_GC_PERCENT=52.381 PRIMER_INTERNAL_18_GC_PERCENT=52.174 PRIMER_INTERNAL_18_SELF_ANY_TH=0.00 PRIMER_LEFT_18_SELF_ANY_TH=0.00 PRIMER_RIGHT_18_SELF_ANY_TH=0.00 PRIMER_INTERNAL_18_SELF_END_TH=0.00 PRIMER_LEFT_18_SELF_END_TH=0.00 PRIMER_RIGHT_18_SELF_END_TH=0.00 PRIMER_LEFT_18_HAIRPIN_TH=40.30 PRIMER_RIGHT_18_HAIRPIN_TH=41.74 PRIMER_INTERNAL_18_HAIRPIN_TH=0.00 PRIMER_LEFT_18_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_18_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_18_END_STABILITY=3.3400 PRIMER_RIGHT_18_END_STABILITY=3.3600 PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=0.7760 PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=1.0131 PRIMER_LEFT_19_PENALTY=1.568623 PRIMER_RIGHT_19_PENALTY=1.997913 PRIMER_INTERNAL_19_PENALTY=3.881423 PRIMER_LEFT_19_SEQUENCE=ATTAGTGGGCCATTCGATCC PRIMER_RIGHT_19_SEQUENCE=GCTATCGATTTGGGTCGGGAT PRIMER_INTERNAL_19_SEQUENCE=TAGTGGGCCATTCGATCCCGA PRIMER_LEFT_19=60,20 PRIMER_RIGHT_19=96,21 PRIMER_INTERNAL_19=62,21 PRIMER_LEFT_19_TM=57.431 PRIMER_RIGHT_19_TM=59.998 PRIMER_INTERNAL_19_TM=57.119 PRIMER_LEFT_19_GC_PERCENT=50.000 PRIMER_RIGHT_19_GC_PERCENT=52.381 PRIMER_INTERNAL_19_GC_PERCENT=57.143 PRIMER_INTERNAL_19_SELF_ANY_TH=0.00 PRIMER_LEFT_19_SELF_ANY_TH=0.00 PRIMER_RIGHT_19_SELF_ANY_TH=1.64 PRIMER_INTERNAL_19_SELF_END_TH=0.00 PRIMER_LEFT_19_SELF_END_TH=0.00 PRIMER_RIGHT_19_SELF_END_TH=0.00 PRIMER_LEFT_19_HAIRPIN_TH=0.00 PRIMER_RIGHT_19_HAIRPIN_TH=41.74 PRIMER_INTERNAL_19_HAIRPIN_TH=41.96 PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence PRIMER_RIGHT_19_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_19_END_STABILITY=3.3600 PRIMER_RIGHT_19_END_STABILITY=3.8500 PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=2.1179 PRIMER_LEFT_20_PENALTY=1.673833 PRIMER_RIGHT_20_PENALTY=2.038681 PRIMER_INTERNAL_20_PENALTY=3.936624 PRIMER_LEFT_20_SEQUENCE=CCGACCCAAATCGATAGCTAC PRIMER_RIGHT_20_SEQUENCE=TCGATTTGGGTCGGGATCGA PRIMER_INTERNAL_20_SEQUENCE=TGGGCCATTCGATCCCGACCCA PRIMER_LEFT_20=79,21 PRIMER_RIGHT_20=92,20 PRIMER_INTERNAL_20=65,22 PRIMER_LEFT_20_TM=58.326 PRIMER_RIGHT_20_TM=61.039 PRIMER_INTERNAL_20_TM=61.937 PRIMER_LEFT_20_GC_PERCENT=52.381 PRIMER_RIGHT_20_GC_PERCENT=55.000 PRIMER_INTERNAL_20_GC_PERCENT=63.636 PRIMER_INTERNAL_20_SELF_ANY_TH=8.58 PRIMER_LEFT_20_SELF_ANY_TH=0.00 PRIMER_RIGHT_20_SELF_ANY_TH=5.62 PRIMER_INTERNAL_20_SELF_END_TH=6.38 PRIMER_LEFT_20_SELF_END_TH=0.00 PRIMER_RIGHT_20_SELF_END_TH=4.35 PRIMER_LEFT_20_HAIRPIN_TH=45.13 PRIMER_RIGHT_20_HAIRPIN_TH=43.86 PRIMER_INTERNAL_20_HAIRPIN_TH=42.67 PRIMER_LEFT_20_LIBRARY_MISPRIMING=11.00, MER4A a consensus PRIMER_RIGHT_20_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_20_END_STABILITY=3.5800 PRIMER_RIGHT_20_END_STABILITY=3.5900 PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=1.5945 PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_21_PENALTY=1.726673 PRIMER_RIGHT_21_PENALTY=2.150848 PRIMER_INTERNAL_21_PENALTY=3.956741 PRIMER_LEFT_21_SEQUENCE=AGACATGACACTGACGATGCA PRIMER_RIGHT_21_SEQUENCE=GCATCGTCAGTGTCATGTCTA PRIMER_INTERNAL_21_SEQUENCE=GGCCATTCGATCCCGACCC PRIMER_LEFT_21=10,21 PRIMER_RIGHT_21=29,21 PRIMER_INTERNAL_21=67,19 PRIMER_LEFT_21_TM=59.727 PRIMER_RIGHT_21_TM=57.849 PRIMER_INTERNAL_21_TM=57.043 PRIMER_LEFT_21_GC_PERCENT=47.619 PRIMER_RIGHT_21_GC_PERCENT=47.619 PRIMER_INTERNAL_21_GC_PERCENT=68.421 PRIMER_INTERNAL_21_SELF_ANY_TH=0.00 PRIMER_LEFT_21_SELF_ANY_TH=0.00 PRIMER_RIGHT_21_SELF_ANY_TH=0.00 PRIMER_INTERNAL_21_SELF_END_TH=0.00 PRIMER_LEFT_21_SELF_END_TH=0.00 PRIMER_RIGHT_21_SELF_END_TH=0.00 PRIMER_LEFT_21_HAIRPIN_TH=0.00 PRIMER_RIGHT_21_HAIRPIN_TH=0.00 PRIMER_INTERNAL_21_HAIRPIN_TH=41.60 PRIMER_LEFT_21_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_21_END_STABILITY=3.9600 PRIMER_RIGHT_21_END_STABILITY=2.5900 PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=5.0696 PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=9.0790 PRIMER_LEFT_22_PENALTY=1.728621 PRIMER_RIGHT_22_PENALTY=2.496898 PRIMER_INTERNAL_22_PENALTY=3.956741 PRIMER_LEFT_22_SEQUENCE=CGATCCCGACCCAAATCGATA PRIMER_RIGHT_22_SEQUENCE=GTCTGCATCGTCAGTGTCA PRIMER_INTERNAL_22_SEQUENCE=GGGCCATTCGATCCCGACC PRIMER_LEFT_22=74,21 PRIMER_RIGHT_22=33,19 PRIMER_INTERNAL_22=66,19 PRIMER_LEFT_22_TM=59.729 PRIMER_RIGHT_22_TM=57.503 PRIMER_INTERNAL_22_TM=57.043 PRIMER_LEFT_22_GC_PERCENT=52.381 PRIMER_RIGHT_22_GC_PERCENT=52.632 PRIMER_INTERNAL_22_GC_PERCENT=68.421 PRIMER_INTERNAL_22_SELF_ANY_TH=2.18 PRIMER_LEFT_22_SELF_ANY_TH=0.00 PRIMER_RIGHT_22_SELF_ANY_TH=0.00 PRIMER_INTERNAL_22_SELF_END_TH=0.00 PRIMER_LEFT_22_SELF_END_TH=0.00 PRIMER_RIGHT_22_SELF_END_TH=0.00 PRIMER_LEFT_22_HAIRPIN_TH=45.13 PRIMER_RIGHT_22_HAIRPIN_TH=0.00 PRIMER_INTERNAL_22_HAIRPIN_TH=41.60 PRIMER_LEFT_22_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_RIGHT_22_LIBRARY_MISPRIMING=12.00, reverse L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence PRIMER_LEFT_22_END_STABILITY=2.9200 PRIMER_RIGHT_22_END_STABILITY=3.5800 PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_23_PENALTY=1.914379 PRIMER_RIGHT_23_PENALTY=2.637998 PRIMER_INTERNAL_23_PENALTY=4.001118 PRIMER_LEFT_23_SEQUENCE=CCCGACCCAAATCGATAGC PRIMER_RIGHT_23_SEQUENCE=TCGTAGCTATCGATTTGGGTC PRIMER_INTERNAL_23_SEQUENCE=CACACACACACACAGCACACAGGT PRIMER_LEFT_23=78,19 PRIMER_RIGHT_23=101,21 PRIMER_INTERNAL_23=36,24 PRIMER_LEFT_23_TM=58.086 PRIMER_RIGHT_23_TM=57.362 PRIMER_INTERNAL_23_TM=60.001 PRIMER_LEFT_23_GC_PERCENT=57.895 PRIMER_RIGHT_23_GC_PERCENT=47.619 PRIMER_INTERNAL_23_GC_PERCENT=54.167 PRIMER_INTERNAL_23_SELF_ANY_TH=0.00 PRIMER_LEFT_23_SELF_ANY_TH=0.00 PRIMER_RIGHT_23_SELF_ANY_TH=14.37 PRIMER_INTERNAL_23_SELF_END_TH=0.00 PRIMER_LEFT_23_SELF_END_TH=0.00 PRIMER_RIGHT_23_SELF_END_TH=0.00 PRIMER_LEFT_23_HAIRPIN_TH=45.13 PRIMER_RIGHT_23_HAIRPIN_TH=0.00 PRIMER_INTERNAL_23_HAIRPIN_TH=0.00 PRIMER_LEFT_23_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR PRIMER_RIGHT_23_LIBRARY_MISPRIMING=11.00, MER4A a consensus PRIMER_LEFT_23_END_STABILITY=2.9700 PRIMER_RIGHT_23_END_STABILITY=4.4600 PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=7.9435 PRIMER_LEFT_24_PENALTY=1.926077 PRIMER_RIGHT_24_PENALTY=2.695830 PRIMER_INTERNAL_24_PENALTY=4.001118 PRIMER_LEFT_24_SEQUENCE=ATTAGTGGGCCATTCGATCCC PRIMER_RIGHT_24_SEQUENCE=GGATCGAATGGCCCACTAATA PRIMER_INTERNAL_24_SEQUENCE=ACACACACACACACAGCACACAGG PRIMER_LEFT_24=60,21 PRIMER_RIGHT_24=79,21 PRIMER_INTERNAL_24=35,24 PRIMER_LEFT_24_TM=59.926 PRIMER_RIGHT_24_TM=57.304 PRIMER_INTERNAL_24_TM=60.001 PRIMER_LEFT_24_GC_PERCENT=52.381 PRIMER_RIGHT_24_GC_PERCENT=47.619 PRIMER_INTERNAL_24_GC_PERCENT=54.167 PRIMER_INTERNAL_24_SELF_ANY_TH=0.00 PRIMER_LEFT_24_SELF_ANY_TH=5.82 PRIMER_RIGHT_24_SELF_ANY_TH=0.00 PRIMER_INTERNAL_24_SELF_END_TH=0.00 PRIMER_LEFT_24_SELF_END_TH=5.82 PRIMER_RIGHT_24_SELF_END_TH=0.00 PRIMER_LEFT_24_HAIRPIN_TH=0.00 PRIMER_RIGHT_24_HAIRPIN_TH=0.00 PRIMER_INTERNAL_24_HAIRPIN_TH=0.00 PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_24_LIBRARY_MISPRIMING=11.00, HSATII Human satellite II DNA PRIMER_LEFT_24_END_STABILITY=3.8500 PRIMER_RIGHT_24_END_STABILITY=0.9800 PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=5.0305 PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=0.8197 PRIMER_LEFT_25_PENALTY=1.960819 PRIMER_RIGHT_25_PENALTY=2.754380 PRIMER_INTERNAL_25_PENALTY=4.058186 PRIMER_LEFT_25_SEQUENCE=GATCCCGACCCAAATCGATAG PRIMER_RIGHT_25_SEQUENCE=CGGGATCGAATGGCCCACTA PRIMER_INTERNAL_25_SEQUENCE=GGCCATTCGATCCCGACCCAAATC PRIMER_LEFT_25=75,21 PRIMER_RIGHT_25=81,20 PRIMER_INTERNAL_25=67,24 PRIMER_LEFT_25_TM=58.039 PRIMER_RIGHT_25_TM=61.754 PRIMER_INTERNAL_25_TM=59.942 PRIMER_LEFT_25_GC_PERCENT=52.381 PRIMER_RIGHT_25_GC_PERCENT=60.000 PRIMER_INTERNAL_25_GC_PERCENT=58.333 PRIMER_INTERNAL_25_SELF_ANY_TH=0.00 PRIMER_LEFT_25_SELF_ANY_TH=0.00 PRIMER_RIGHT_25_SELF_ANY_TH=14.03 PRIMER_INTERNAL_25_SELF_END_TH=0.00 PRIMER_LEFT_25_SELF_END_TH=0.00 PRIMER_RIGHT_25_SELF_END_TH=0.00 PRIMER_LEFT_25_HAIRPIN_TH=45.13 PRIMER_RIGHT_25_HAIRPIN_TH=39.00 PRIMER_INTERNAL_25_HAIRPIN_TH=41.60 PRIMER_LEFT_25_LIBRARY_MISPRIMING=10.00, MSTa consensus sequence PRIMER_RIGHT_25_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_25_END_STABILITY=2.0800 PRIMER_RIGHT_25_END_STABILITY=2.7400 PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=9.7066 PRIMER_LEFT_26_PENALTY=1.997913 PRIMER_RIGHT_26_PENALTY=2.831715 PRIMER_INTERNAL_26_PENALTY=4.099823 PRIMER_LEFT_26_SEQUENCE=ATCCCGACCCAAATCGATAGC PRIMER_RIGHT_26_SEQUENCE=TCGTCAGTGTCATGTCTACTG PRIMER_INTERNAL_26_SEQUENCE=GGGCCATTCGATCCCGACCCAAA PRIMER_LEFT_26=76,21 PRIMER_RIGHT_26=26,21 PRIMER_INTERNAL_26=66,23 PRIMER_LEFT_26_TM=59.998 PRIMER_RIGHT_26_TM=57.168 PRIMER_INTERNAL_26_TM=61.100 PRIMER_LEFT_26_GC_PERCENT=52.381 PRIMER_RIGHT_26_GC_PERCENT=47.619 PRIMER_INTERNAL_26_GC_PERCENT=60.870 PRIMER_INTERNAL_26_SELF_ANY_TH=2.16 PRIMER_LEFT_26_SELF_ANY_TH=0.00 PRIMER_RIGHT_26_SELF_ANY_TH=15.66 PRIMER_INTERNAL_26_SELF_END_TH=0.00 PRIMER_LEFT_26_SELF_END_TH=0.00 PRIMER_RIGHT_26_SELF_END_TH=15.66 PRIMER_LEFT_26_HAIRPIN_TH=45.13 PRIMER_RIGHT_26_HAIRPIN_TH=0.00 PRIMER_INTERNAL_26_HAIRPIN_TH=41.60 PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR PRIMER_RIGHT_26_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_26_END_STABILITY=2.9700 PRIMER_RIGHT_26_END_STABILITY=2.7400 PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=24.9329 PRIMER_LEFT_27_PENALTY=2.038681 PRIMER_RIGHT_27_PENALTY=2.831715 PRIMER_INTERNAL_27_PENALTY=4.159384 PRIMER_LEFT_27_SEQUENCE=TCGATCCCGACCCAAATCGA PRIMER_RIGHT_27_SEQUENCE=CGTCAGTGTCATGTCTACTGA PRIMER_INTERNAL_27_SEQUENCE=CACACACACACAGCACACAGGT PRIMER_LEFT_27=73,20 PRIMER_RIGHT_27=25,21 PRIMER_INTERNAL_27=38,22 PRIMER_LEFT_27_TM=61.039 PRIMER_RIGHT_27_TM=57.168 PRIMER_INTERNAL_27_TM=57.841 PRIMER_LEFT_27_GC_PERCENT=55.000 PRIMER_RIGHT_27_GC_PERCENT=47.619 PRIMER_INTERNAL_27_GC_PERCENT=54.545 PRIMER_INTERNAL_27_SELF_ANY_TH=0.00 PRIMER_LEFT_27_SELF_ANY_TH=4.17 PRIMER_RIGHT_27_SELF_ANY_TH=19.25 PRIMER_INTERNAL_27_SELF_END_TH=0.00 PRIMER_LEFT_27_SELF_END_TH=2.87 PRIMER_RIGHT_27_SELF_END_TH=17.68 PRIMER_LEFT_27_HAIRPIN_TH=45.13 PRIMER_RIGHT_27_HAIRPIN_TH=0.00 PRIMER_INTERNAL_27_HAIRPIN_TH=0.00 PRIMER_LEFT_27_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER44C Nonautonomous DNA transposon PRIMER_LEFT_27_END_STABILITY=3.5900 PRIMER_RIGHT_27_END_STABILITY=3.4100 PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=3.8324 PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=25.4277 PRIMER_LEFT_28_PENALTY=2.150848 PRIMER_RIGHT_28_PENALTY=2.912899 PRIMER_INTERNAL_28_PENALTY=4.159384 PRIMER_LEFT_28_SEQUENCE=TAGACATGACACTGACGATGC PRIMER_RIGHT_28_SEQUENCE=TGCATCGTCAGTGTCATGT PRIMER_INTERNAL_28_SEQUENCE=ACACACACACACAGCACACAGG PRIMER_LEFT_28=9,21 PRIMER_RIGHT_28=30,19 PRIMER_INTERNAL_28=37,22 PRIMER_LEFT_28_TM=57.849 PRIMER_RIGHT_28_TM=57.087 PRIMER_INTERNAL_28_TM=57.841 PRIMER_LEFT_28_GC_PERCENT=47.619 PRIMER_RIGHT_28_GC_PERCENT=47.368 PRIMER_INTERNAL_28_GC_PERCENT=54.545 PRIMER_INTERNAL_28_SELF_ANY_TH=0.00 PRIMER_LEFT_28_SELF_ANY_TH=3.11 PRIMER_RIGHT_28_SELF_ANY_TH=3.35 PRIMER_INTERNAL_28_SELF_END_TH=0.00 PRIMER_LEFT_28_SELF_END_TH=0.00 PRIMER_RIGHT_28_SELF_END_TH=0.00 PRIMER_LEFT_28_HAIRPIN_TH=0.00 PRIMER_RIGHT_28_HAIRPIN_TH=0.00 PRIMER_INTERNAL_28_HAIRPIN_TH=0.00 PRIMER_LEFT_28_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_28_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_28_END_STABILITY=3.9100 PRIMER_RIGHT_28_END_STABILITY=3.2100 PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=12.1923 PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_29_PENALTY=2.469564 PRIMER_RIGHT_29_PENALTY=2.989104 PRIMER_INTERNAL_29_PENALTY=4.196497 PRIMER_LEFT_29_SEQUENCE=CATTCGATCCCGACCCAAA PRIMER_RIGHT_29_SEQUENCE=AGCTATCGATTTGGGTCGG PRIMER_INTERNAL_29_SEQUENCE=CACACACACACACAGCACACAGG PRIMER_LEFT_29=70,19 PRIMER_RIGHT_29=97,19 PRIMER_INTERNAL_29=36,23 PRIMER_LEFT_29_TM=57.530 PRIMER_RIGHT_29_TM=57.011 PRIMER_INTERNAL_29_TM=58.804 PRIMER_LEFT_29_GC_PERCENT=52.632 PRIMER_RIGHT_29_GC_PERCENT=52.632 PRIMER_INTERNAL_29_GC_PERCENT=56.522 PRIMER_INTERNAL_29_SELF_ANY_TH=0.00 PRIMER_LEFT_29_SELF_ANY_TH=0.00 PRIMER_RIGHT_29_SELF_ANY_TH=0.00 PRIMER_INTERNAL_29_SELF_END_TH=0.00 PRIMER_LEFT_29_SELF_END_TH=0.00 PRIMER_RIGHT_29_SELF_END_TH=0.00 PRIMER_LEFT_29_HAIRPIN_TH=45.50 PRIMER_RIGHT_29_HAIRPIN_TH=41.74 PRIMER_INTERNAL_29_HAIRPIN_TH=0.00 PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_LEFT_29_END_STABILITY=3.2800 PRIMER_RIGHT_29_END_STABILITY=4.7900 PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=8.0154 PRIMER_LEFT_30_PENALTY=2.476833 PRIMER_RIGHT_30_PENALTY=3.032680 PRIMER_INTERNAL_30_PENALTY=4.232604 PRIMER_LEFT_30_SEQUENCE=CCATTCGATCCCGACCCAAAT PRIMER_RIGHT_30_SEQUENCE=AGCTATCGATTTGGGTCGGGA PRIMER_INTERNAL_30_SEQUENCE=GCCATTCGATCCCGACCCAAATCG PRIMER_LEFT_30=69,21 PRIMER_RIGHT_30=97,21 PRIMER_INTERNAL_30=68,24 PRIMER_LEFT_30_TM=60.477 PRIMER_RIGHT_30_TM=61.033 PRIMER_INTERNAL_30_TM=60.233 PRIMER_LEFT_30_GC_PERCENT=52.381 PRIMER_RIGHT_30_GC_PERCENT=52.381 PRIMER_INTERNAL_30_GC_PERCENT=58.333 PRIMER_INTERNAL_30_SELF_ANY_TH=0.00 PRIMER_LEFT_30_SELF_ANY_TH=0.00 PRIMER_RIGHT_30_SELF_ANY_TH=0.00 PRIMER_INTERNAL_30_SELF_END_TH=0.00 PRIMER_LEFT_30_SELF_END_TH=0.00 PRIMER_RIGHT_30_SELF_END_TH=0.00 PRIMER_LEFT_30_HAIRPIN_TH=45.50 PRIMER_RIGHT_30_HAIRPIN_TH=41.74 PRIMER_INTERNAL_30_HAIRPIN_TH=41.60 PRIMER_LEFT_30_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_30_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_30_END_STABILITY=2.3200 PRIMER_RIGHT_30_END_STABILITY=5.1400 PRIMER_LEFT_30_TEMPLATE_MISPRIMING_TH=4.3679 PRIMER_RIGHT_30_TEMPLATE_MISPRIMING_TH=2.6099 PRIMER_LEFT_31_PENALTY=2.496898 PRIMER_RIGHT_31_PENALTY=3.367844 PRIMER_INTERNAL_31_PENALTY=4.351864 PRIMER_LEFT_31_SEQUENCE=TGACACTGACGATGCAGAC PRIMER_RIGHT_31_SEQUENCE=ATCGATTTGGGTCGGGATCGA PRIMER_INTERNAL_31_SEQUENCE=TTAGTGGGCCATTCGATCCCGACC PRIMER_LEFT_31=15,19 PRIMER_RIGHT_31=93,21 PRIMER_INTERNAL_31=61,24 PRIMER_LEFT_31_TM=57.503 PRIMER_RIGHT_31_TM=61.368 PRIMER_INTERNAL_31_TM=60.352 PRIMER_LEFT_31_GC_PERCENT=52.632 PRIMER_RIGHT_31_GC_PERCENT=52.381 PRIMER_INTERNAL_31_GC_PERCENT=58.333 PRIMER_INTERNAL_31_SELF_ANY_TH=5.61 PRIMER_LEFT_31_SELF_ANY_TH=4.19 PRIMER_RIGHT_31_SELF_ANY_TH=7.64 PRIMER_INTERNAL_31_SELF_END_TH=0.00 PRIMER_LEFT_31_SELF_END_TH=0.00 PRIMER_RIGHT_31_SELF_END_TH=7.64 PRIMER_LEFT_31_HAIRPIN_TH=0.00 PRIMER_RIGHT_31_HAIRPIN_TH=43.86 PRIMER_INTERNAL_31_HAIRPIN_TH=41.60 PRIMER_LEFT_31_LIBRARY_MISPRIMING=11.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_31_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_31_END_STABILITY=3.5100 PRIMER_RIGHT_31_END_STABILITY=3.5900 PRIMER_LEFT_31_TEMPLATE_MISPRIMING_TH=8.4593 PRIMER_RIGHT_31_TEMPLATE_MISPRIMING_TH=1.6205 PRIMER_LEFT_32_PENALTY=2.540363 PRIMER_RIGHT_32_PENALTY=4.101796 PRIMER_INTERNAL_32_PENALTY=4.478673 PRIMER_LEFT_32_SEQUENCE=ATTCGATCCCGACCCAAATCG PRIMER_RIGHT_32_SEQUENCE=TCGGGATCGAATGGCCCAC PRIMER_INTERNAL_32_SEQUENCE=TTAGTGGGCCATTCGATCCCGAC PRIMER_LEFT_32=71,21 PRIMER_RIGHT_32=82,19 PRIMER_INTERNAL_32=61,23 PRIMER_LEFT_32_TM=60.540 PRIMER_RIGHT_32_TM=62.102 PRIMER_INTERNAL_32_TM=58.521 PRIMER_LEFT_32_GC_PERCENT=52.381 PRIMER_RIGHT_32_GC_PERCENT=63.158 PRIMER_INTERNAL_32_GC_PERCENT=56.522 PRIMER_INTERNAL_32_SELF_ANY_TH=3.57 PRIMER_LEFT_32_SELF_ANY_TH=0.00 PRIMER_RIGHT_32_SELF_ANY_TH=14.03 PRIMER_INTERNAL_32_SELF_END_TH=3.02 PRIMER_LEFT_32_SELF_END_TH=0.00 PRIMER_RIGHT_32_SELF_END_TH=0.00 PRIMER_LEFT_32_HAIRPIN_TH=45.50 PRIMER_RIGHT_32_HAIRPIN_TH=43.03 PRIMER_INTERNAL_32_HAIRPIN_TH=41.60 PRIMER_LEFT_32_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_RIGHT_32_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_32_END_STABILITY=3.3400 PRIMER_RIGHT_32_END_STABILITY=4.6100 PRIMER_LEFT_32_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_32_TEMPLATE_MISPRIMING_TH=11.0013 PRIMER_LEFT_33_PENALTY=2.637998 PRIMER_RIGHT_33_PENALTY=4.106986 PRIMER_INTERNAL_33_PENALTY=4.484274 PRIMER_LEFT_33_SEQUENCE=GACCCAAATCGATAGCTACGA PRIMER_RIGHT_33_SEQUENCE=CGGGATCGAATGGCCCACT PRIMER_INTERNAL_33_SEQUENCE=TTAGTGGGCCATTCGATCCCGA PRIMER_LEFT_33=81,21 PRIMER_RIGHT_33=81,19 PRIMER_INTERNAL_33=61,22 PRIMER_LEFT_33_TM=57.362 PRIMER_RIGHT_33_TM=62.107 PRIMER_INTERNAL_33_TM=57.516 PRIMER_LEFT_33_GC_PERCENT=47.619 PRIMER_RIGHT_33_GC_PERCENT=63.158 PRIMER_INTERNAL_33_GC_PERCENT=54.545 PRIMER_INTERNAL_33_SELF_ANY_TH=0.00 PRIMER_LEFT_33_SELF_ANY_TH=16.85 PRIMER_RIGHT_33_SELF_ANY_TH=14.03 PRIMER_INTERNAL_33_SELF_END_TH=0.00 PRIMER_LEFT_33_SELF_END_TH=16.85 PRIMER_RIGHT_33_SELF_END_TH=0.00 PRIMER_LEFT_33_HAIRPIN_TH=43.65 PRIMER_RIGHT_33_HAIRPIN_TH=39.00 PRIMER_INTERNAL_33_HAIRPIN_TH=41.96 PRIMER_LEFT_33_LIBRARY_MISPRIMING=11.00, MER4A a consensus PRIMER_RIGHT_33_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_33_END_STABILITY=3.4300 PRIMER_RIGHT_33_END_STABILITY=4.0000 PRIMER_LEFT_33_TEMPLATE_MISPRIMING_TH=2.4413 PRIMER_RIGHT_33_TEMPLATE_MISPRIMING_TH=10.5305 PRIMER_LEFT_34_PENALTY=2.695830 PRIMER_RIGHT_34_PENALTY=4.250296 PRIMER_INTERNAL_34_PENALTY=4.552303 PRIMER_LEFT_34_SEQUENCE=TATTAGTGGGCCATTCGATCC PRIMER_RIGHT_34_SEQUENCE=CGGGATCGAATGGCCCACTAA PRIMER_INTERNAL_34_SEQUENCE=ACACACACACACACAGCACACA PRIMER_LEFT_34=59,21 PRIMER_RIGHT_34=81,21 PRIMER_INTERNAL_34=35,22 PRIMER_LEFT_34_TM=57.304 PRIMER_RIGHT_34_TM=62.250 PRIMER_INTERNAL_34_TM=57.448 PRIMER_LEFT_34_GC_PERCENT=47.619 PRIMER_RIGHT_34_GC_PERCENT=57.143 PRIMER_INTERNAL_34_GC_PERCENT=50.000 PRIMER_INTERNAL_34_SELF_ANY_TH=0.00 PRIMER_LEFT_34_SELF_ANY_TH=0.00 PRIMER_RIGHT_34_SELF_ANY_TH=14.03 PRIMER_INTERNAL_34_SELF_END_TH=0.00 PRIMER_LEFT_34_SELF_END_TH=0.00 PRIMER_RIGHT_34_SELF_END_TH=0.00 PRIMER_LEFT_34_HAIRPIN_TH=0.00 PRIMER_RIGHT_34_HAIRPIN_TH=39.00 PRIMER_INTERNAL_34_HAIRPIN_TH=0.00 PRIMER_LEFT_34_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence PRIMER_RIGHT_34_LIBRARY_MISPRIMING=10.00, HSATII Human satellite II DNA PRIMER_LEFT_34_END_STABILITY=3.3600 PRIMER_RIGHT_34_END_STABILITY=2.2400 PRIMER_LEFT_34_TEMPLATE_MISPRIMING_TH=1.4539 PRIMER_RIGHT_34_TEMPLATE_MISPRIMING_TH=10.9108 PRIMER_LEFT_35_PENALTY=2.754380 PRIMER_INTERNAL_35_PENALTY=4.639434 PRIMER_LEFT_35_SEQUENCE=TAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_35_SEQUENCE=GCCATTCGATCCCGACCCAAAT PRIMER_LEFT_35=62,20 PRIMER_INTERNAL_35=68,22 PRIMER_LEFT_35_TM=61.754 PRIMER_INTERNAL_35_TM=57.361 PRIMER_LEFT_35_GC_PERCENT=60.000 PRIMER_INTERNAL_35_GC_PERCENT=54.545 PRIMER_INTERNAL_35_SELF_ANY_TH=0.00 PRIMER_LEFT_35_SELF_ANY_TH=7.70 PRIMER_INTERNAL_35_SELF_END_TH=0.00 PRIMER_LEFT_35_SELF_END_TH=0.00 PRIMER_LEFT_35_HAIRPIN_TH=37.07 PRIMER_INTERNAL_35_HAIRPIN_TH=41.60 PRIMER_LEFT_35_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence PRIMER_LEFT_35_END_STABILITY=5.1400 PRIMER_LEFT_35_TEMPLATE_MISPRIMING_TH=6.4478 PRIMER_LEFT_36_PENALTY=2.772619 PRIMER_INTERNAL_36_PENALTY=4.809017 PRIMER_LEFT_36_SEQUENCE=TTCGATCCCGACCCAAATC PRIMER_INTERNAL_36_SEQUENCE=ACACACACACACACAGCACACAG PRIMER_LEFT_36=72,19 PRIMER_INTERNAL_36=35,23 PRIMER_LEFT_36_TM=57.227 PRIMER_INTERNAL_36_TM=58.191 PRIMER_LEFT_36_GC_PERCENT=52.632 PRIMER_INTERNAL_36_GC_PERCENT=52.174 PRIMER_INTERNAL_36_SELF_ANY_TH=0.00 PRIMER_LEFT_36_SELF_ANY_TH=0.00 PRIMER_INTERNAL_36_SELF_END_TH=0.00 PRIMER_LEFT_36_SELF_END_TH=0.00 PRIMER_LEFT_36_HAIRPIN_TH=45.50 PRIMER_INTERNAL_36_HAIRPIN_TH=0.00 PRIMER_LEFT_36_LIBRARY_MISPRIMING=12.00, THE1b consensus sequence PRIMER_LEFT_36_END_STABILITY=2.1700 PRIMER_LEFT_36_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_37_PENALTY=2.831715 PRIMER_INTERNAL_37_PENALTY=4.881095 PRIMER_LEFT_37_SEQUENCE=CAGTAGACATGACACTGACGA PRIMER_INTERNAL_37_SEQUENCE=GCCATTCGATCCCGACCCAAATC PRIMER_LEFT_37=6,21 PRIMER_INTERNAL_37=68,23 PRIMER_LEFT_37_TM=57.168 PRIMER_INTERNAL_37_TM=58.119 PRIMER_LEFT_37_GC_PERCENT=47.619 PRIMER_INTERNAL_37_GC_PERCENT=56.522 PRIMER_INTERNAL_37_SELF_ANY_TH=0.00 PRIMER_LEFT_37_SELF_ANY_TH=16.46 PRIMER_INTERNAL_37_SELF_END_TH=0.00 PRIMER_LEFT_37_SELF_END_TH=0.00 PRIMER_LEFT_37_HAIRPIN_TH=0.00 PRIMER_INTERNAL_37_HAIRPIN_TH=41.60 PRIMER_LEFT_37_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_37_END_STABILITY=4.2000 PRIMER_LEFT_37_TEMPLATE_MISPRIMING_TH=19.8783 PRIMER_LEFT_38_PENALTY=2.831715 PRIMER_INTERNAL_38_PENALTY=5.108025 PRIMER_LEFT_38_SEQUENCE=TCAGTAGACATGACACTGACG PRIMER_INTERNAL_38_SEQUENCE=TGGGCCATTCGATCCCGACCCAA PRIMER_LEFT_38=5,21 PRIMER_INTERNAL_38=65,23 PRIMER_LEFT_38_TM=57.168 PRIMER_INTERNAL_38_TM=62.108 PRIMER_LEFT_38_GC_PERCENT=47.619 PRIMER_INTERNAL_38_GC_PERCENT=60.870 PRIMER_INTERNAL_38_SELF_ANY_TH=8.58 PRIMER_LEFT_38_SELF_ANY_TH=20.80 PRIMER_INTERNAL_38_SELF_END_TH=0.00 PRIMER_LEFT_38_SELF_END_TH=6.93 PRIMER_LEFT_38_HAIRPIN_TH=0.00 PRIMER_INTERNAL_38_HAIRPIN_TH=41.29 PRIMER_LEFT_38_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_38_END_STABILITY=4.3500 PRIMER_LEFT_38_TEMPLATE_MISPRIMING_TH=6.9281 PRIMER_LEFT_39_PENALTY=2.912899 PRIMER_INTERNAL_39_PENALTY=5.111368 PRIMER_LEFT_39_SEQUENCE=ACATGACACTGACGATGCA PRIMER_INTERNAL_39_SEQUENCE=CCATTCGATCCCGACCCAAATCGA PRIMER_LEFT_39=12,19 PRIMER_INTERNAL_39=69,24 PRIMER_LEFT_39_TM=57.087 PRIMER_INTERNAL_39_TM=58.889 PRIMER_LEFT_39_GC_PERCENT=47.368 PRIMER_INTERNAL_39_GC_PERCENT=54.167 PRIMER_INTERNAL_39_SELF_ANY_TH=0.00 PRIMER_LEFT_39_SELF_ANY_TH=0.00 PRIMER_INTERNAL_39_SELF_END_TH=0.00 PRIMER_LEFT_39_SELF_END_TH=0.00 PRIMER_LEFT_39_HAIRPIN_TH=0.00 PRIMER_INTERNAL_39_HAIRPIN_TH=41.60 PRIMER_LEFT_39_LIBRARY_MISPRIMING=12.00, MER25 Older LINE1 5' end region - a consensus PRIMER_LEFT_39_END_STABILITY=3.9600 PRIMER_LEFT_39_TEMPLATE_MISPRIMING_TH=8.1705 PRIMER_LEFT_40_PENALTY=2.989104 PRIMER_INTERNAL_40_PENALTY=5.111455 PRIMER_LEFT_40_SEQUENCE=CCGACCCAAATCGATAGCT PRIMER_INTERNAL_40_SEQUENCE=CCATTCGATCCCGACCCAAATCG PRIMER_LEFT_40=79,19 PRIMER_INTERNAL_40=69,23 PRIMER_LEFT_40_TM=57.011 PRIMER_INTERNAL_40_TM=57.889 PRIMER_LEFT_40_GC_PERCENT=52.632 PRIMER_INTERNAL_40_GC_PERCENT=56.522 PRIMER_INTERNAL_40_SELF_ANY_TH=0.00 PRIMER_LEFT_40_SELF_ANY_TH=0.00 PRIMER_INTERNAL_40_SELF_END_TH=0.00 PRIMER_LEFT_40_SELF_END_TH=0.00 PRIMER_LEFT_40_HAIRPIN_TH=45.13 PRIMER_INTERNAL_40_HAIRPIN_TH=41.60 PRIMER_LEFT_40_LIBRARY_MISPRIMING=9.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR PRIMER_LEFT_40_END_STABILITY=3.3200 PRIMER_LEFT_40_TEMPLATE_MISPRIMING_TH=5.9515 PRIMER_LEFT_41_PENALTY=3.032680 PRIMER_INTERNAL_41_PENALTY=5.114088 PRIMER_LEFT_41_SEQUENCE=TCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_41_SEQUENCE=ACAGCACACAGGTATTAGTGGGCCA PRIMER_LEFT_41=77,21 PRIMER_INTERNAL_41=47,25 PRIMER_LEFT_41_TM=61.033 PRIMER_INTERNAL_41_TM=59.886 PRIMER_LEFT_41_GC_PERCENT=52.381 PRIMER_INTERNAL_41_GC_PERCENT=52.000 PRIMER_INTERNAL_41_SELF_ANY_TH=8.63 PRIMER_LEFT_41_SELF_ANY_TH=0.00 PRIMER_INTERNAL_41_SELF_END_TH=1.50 PRIMER_LEFT_41_SELF_END_TH=0.00 PRIMER_LEFT_41_HAIRPIN_TH=45.13 PRIMER_INTERNAL_41_HAIRPIN_TH=32.68 PRIMER_LEFT_41_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_LEFT_41_END_STABILITY=3.3200 PRIMER_LEFT_41_TEMPLATE_MISPRIMING_TH=1.5271 PRIMER_LEFT_42_PENALTY=3.367844 PRIMER_INTERNAL_42_PENALTY=5.122599 PRIMER_LEFT_42_SEQUENCE=TCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_42_SEQUENCE=GGGCCATTCGATCCCGACCCAAAT PRIMER_LEFT_42=73,21 PRIMER_INTERNAL_42=66,24 PRIMER_LEFT_42_TM=61.368 PRIMER_INTERNAL_42_TM=61.123 PRIMER_LEFT_42_GC_PERCENT=52.381 PRIMER_INTERNAL_42_GC_PERCENT=58.333 PRIMER_INTERNAL_42_SELF_ANY_TH=2.16 PRIMER_LEFT_42_SELF_ANY_TH=3.83 PRIMER_INTERNAL_42_SELF_END_TH=0.00 PRIMER_LEFT_42_SELF_END_TH=0.00 PRIMER_LEFT_42_HAIRPIN_TH=45.13 PRIMER_INTERNAL_42_HAIRPIN_TH=41.60 PRIMER_LEFT_42_LIBRARY_MISPRIMING=11.00, THE1b consensus sequence PRIMER_LEFT_42_END_STABILITY=3.5900 PRIMER_LEFT_42_TEMPLATE_MISPRIMING_TH=5.3068 PRIMER_LEFT_43_PENALTY=3.568322 PRIMER_INTERNAL_43_PENALTY=5.268172 PRIMER_LEFT_43_SEQUENCE=TTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_43_SEQUENCE=AGCACACAGGTATTAGTGGGCCA PRIMER_LEFT_43=72,21 PRIMER_INTERNAL_43=49,23 PRIMER_LEFT_43_TM=61.568 PRIMER_INTERNAL_43_TM=57.732 PRIMER_LEFT_43_GC_PERCENT=52.381 PRIMER_INTERNAL_43_GC_PERCENT=52.174 PRIMER_INTERNAL_43_SELF_ANY_TH=8.63 PRIMER_LEFT_43_SELF_ANY_TH=4.17 PRIMER_INTERNAL_43_SELF_END_TH=1.50 PRIMER_LEFT_43_SELF_END_TH=3.06 PRIMER_LEFT_43_HAIRPIN_TH=45.50 PRIMER_INTERNAL_43_HAIRPIN_TH=32.68 PRIMER_LEFT_43_LIBRARY_MISPRIMING=10.00, THE1b consensus sequence PRIMER_LEFT_43_END_STABILITY=3.5900 PRIMER_LEFT_43_TEMPLATE_MISPRIMING_TH=3.8324 PRIMER_LEFT_44_PENALTY=3.602746 PRIMER_INTERNAL_44_PENALTY=5.295331 PRIMER_LEFT_44_SEQUENCE=GCCATTCGATCCCGACCCAA PRIMER_INTERNAL_44_SEQUENCE=ACACACACACACAGCACACAGGTA PRIMER_LEFT_44=68,20 PRIMER_INTERNAL_44=37,24 PRIMER_LEFT_44_TM=62.603 PRIMER_INTERNAL_44_TM=58.705 PRIMER_LEFT_44_GC_PERCENT=60.000 PRIMER_INTERNAL_44_GC_PERCENT=50.000 PRIMER_INTERNAL_44_SELF_ANY_TH=0.00 PRIMER_LEFT_44_SELF_ANY_TH=0.00 PRIMER_INTERNAL_44_SELF_END_TH=0.00 PRIMER_LEFT_44_SELF_END_TH=0.00 PRIMER_LEFT_44_HAIRPIN_TH=45.50 PRIMER_INTERNAL_44_HAIRPIN_TH=0.00 PRIMER_LEFT_44_LIBRARY_MISPRIMING=10.00, MER4A a consensus PRIMER_LEFT_44_END_STABILITY=4.1200 PRIMER_LEFT_44_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_45_PENALTY=4.101796 PRIMER_INTERNAL_45_PENALTY=5.306369 PRIMER_LEFT_45_SEQUENCE=GCCATTCGATCCCGACCCA PRIMER_INTERNAL_45_SEQUENCE=CAGCACACAGGTATTAGTGGGCCA PRIMER_LEFT_45=68,19 PRIMER_INTERNAL_45=48,24 PRIMER_LEFT_45_TM=62.102 PRIMER_INTERNAL_45_TM=58.694 PRIMER_LEFT_45_GC_PERCENT=63.158 PRIMER_INTERNAL_45_GC_PERCENT=54.167 PRIMER_INTERNAL_45_SELF_ANY_TH=8.63 PRIMER_LEFT_45_SELF_ANY_TH=0.00 PRIMER_INTERNAL_45_SELF_END_TH=1.50 PRIMER_LEFT_45_SELF_END_TH=0.00 PRIMER_LEFT_45_HAIRPIN_TH=45.50 PRIMER_INTERNAL_45_HAIRPIN_TH=32.68 PRIMER_LEFT_45_LIBRARY_MISPRIMING=10.00, TCCCA Primate simple repeat pattern PRIMER_LEFT_45_END_STABILITY=4.5100 PRIMER_LEFT_45_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_46_PENALTY=4.101796 PRIMER_INTERNAL_46_PENALTY=5.307164 PRIMER_LEFT_46_SEQUENCE=TGGGCCATTCGATCCCGAC PRIMER_INTERNAL_46_SEQUENCE=ACAGCACACAGGTATTAGTGGGCC PRIMER_LEFT_46=65,19 PRIMER_INTERNAL_46=47,24 PRIMER_LEFT_46_TM=62.102 PRIMER_INTERNAL_46_TM=58.693 PRIMER_LEFT_46_GC_PERCENT=63.158 PRIMER_INTERNAL_46_GC_PERCENT=54.167 PRIMER_INTERNAL_46_SELF_ANY_TH=8.63 PRIMER_LEFT_46_SELF_ANY_TH=7.70 PRIMER_INTERNAL_46_SELF_END_TH=0.00 PRIMER_LEFT_46_SELF_END_TH=0.00 PRIMER_LEFT_46_HAIRPIN_TH=45.50 PRIMER_INTERNAL_46_HAIRPIN_TH=32.68 PRIMER_LEFT_46_LIBRARY_MISPRIMING=9.00, reverse LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat PRIMER_LEFT_46_END_STABILITY=4.7900 PRIMER_LEFT_46_TEMPLATE_MISPRIMING_TH=6.4764 PRIMER_LEFT_47_PENALTY=4.101796 PRIMER_INTERNAL_47_PENALTY=5.316271 PRIMER_LEFT_47_SEQUENCE=GTGGGCCATTCGATCCCGA PRIMER_INTERNAL_47_SEQUENCE=ATTAGTGGGCCATTCGATCCCGA PRIMER_LEFT_47=64,19 PRIMER_INTERNAL_47=60,23 PRIMER_LEFT_47_TM=62.102 PRIMER_INTERNAL_47_TM=57.684 PRIMER_LEFT_47_GC_PERCENT=63.158 PRIMER_INTERNAL_47_GC_PERCENT=52.174 PRIMER_INTERNAL_47_SELF_ANY_TH=0.00 PRIMER_LEFT_47_SELF_ANY_TH=5.82 PRIMER_INTERNAL_47_SELF_END_TH=0.00 PRIMER_LEFT_47_SELF_END_TH=0.00 PRIMER_LEFT_47_HAIRPIN_TH=43.96 PRIMER_INTERNAL_47_HAIRPIN_TH=41.96 PRIMER_LEFT_47_LIBRARY_MISPRIMING=9.00, MER1B Nonautonomous DNA transposon PRIMER_LEFT_47_END_STABILITY=5.1400 PRIMER_LEFT_47_TEMPLATE_MISPRIMING_TH=10.7677 PRIMER_LEFT_48_PENALTY=4.106986 PRIMER_INTERNAL_48_PENALTY=5.357946 PRIMER_LEFT_48_SEQUENCE=AGTGGGCCATTCGATCCCG PRIMER_INTERNAL_48_SEQUENCE=ATTAGTGGGCCATTCGATCCCGAC PRIMER_LEFT_48=63,19 PRIMER_INTERNAL_48=60,24 PRIMER_LEFT_48_TM=62.107 PRIMER_INTERNAL_48_TM=58.642 PRIMER_LEFT_48_GC_PERCENT=63.158 PRIMER_INTERNAL_48_GC_PERCENT=54.167 PRIMER_INTERNAL_48_SELF_ANY_TH=3.57 PRIMER_LEFT_48_SELF_ANY_TH=7.70 PRIMER_INTERNAL_48_SELF_END_TH=3.02 PRIMER_LEFT_48_SELF_END_TH=0.00 PRIMER_LEFT_48_HAIRPIN_TH=37.07 PRIMER_INTERNAL_48_HAIRPIN_TH=41.60 PRIMER_LEFT_48_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence PRIMER_LEFT_48_END_STABILITY=5.1400 PRIMER_LEFT_48_TEMPLATE_MISPRIMING_TH=6.4478 PRIMER_LEFT_49_PENALTY=4.250296 PRIMER_INTERNAL_49_PENALTY=5.401146 PRIMER_LEFT_49_SEQUENCE=TTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_49_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACC PRIMER_LEFT_49=61,21 PRIMER_INTERNAL_49=60,25 PRIMER_LEFT_49_TM=62.250 PRIMER_INTERNAL_49_TM=60.401 PRIMER_LEFT_49_GC_PERCENT=57.143 PRIMER_INTERNAL_49_GC_PERCENT=56.000 PRIMER_INTERNAL_49_SELF_ANY_TH=5.61 PRIMER_LEFT_49_SELF_ANY_TH=7.70 PRIMER_INTERNAL_49_SELF_END_TH=0.00 PRIMER_LEFT_49_SELF_END_TH=0.00 PRIMER_LEFT_49_HAIRPIN_TH=37.07 PRIMER_INTERNAL_49_HAIRPIN_TH=41.60 PRIMER_LEFT_49_LIBRARY_MISPRIMING=9.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence PRIMER_LEFT_49_END_STABILITY=5.1400 PRIMER_LEFT_49_TEMPLATE_MISPRIMING_TH=6.4478 PRIMER_LEFT_50_PENALTY=4.826926 PRIMER_INTERNAL_50_PENALTY=5.439697 PRIMER_LEFT_50_SEQUENCE=GGCCATTCGATCCCGACCC PRIMER_INTERNAL_50_SEQUENCE=CACACACACACACAGCACACAGGTA PRIMER_LEFT_50=67,19 PRIMER_INTERNAL_50=36,25 PRIMER_LEFT_50_TM=62.827 PRIMER_INTERNAL_50_TM=59.560 PRIMER_LEFT_50_GC_PERCENT=68.421 PRIMER_INTERNAL_50_GC_PERCENT=52.000 PRIMER_INTERNAL_50_SELF_ANY_TH=0.00 PRIMER_LEFT_50_SELF_ANY_TH=0.00 PRIMER_INTERNAL_50_SELF_END_TH=0.00 PRIMER_LEFT_50_SELF_END_TH=0.00 PRIMER_LEFT_50_HAIRPIN_TH=45.50 PRIMER_INTERNAL_50_HAIRPIN_TH=0.00 PRIMER_LEFT_50_LIBRARY_MISPRIMING=9.00, TCCCA Primate simple repeat pattern PRIMER_LEFT_50_END_STABILITY=4.4600 PRIMER_LEFT_50_TEMPLATE_MISPRIMING_TH=3.6740 PRIMER_LEFT_51_PENALTY=4.826926 PRIMER_INTERNAL_51_PENALTY=5.444425 PRIMER_LEFT_51_SEQUENCE=GGGCCATTCGATCCCGACC PRIMER_INTERNAL_51_SEQUENCE=CACAGCACACAGGTATTAGTGGGCC PRIMER_LEFT_51=66,19 PRIMER_INTERNAL_51=46,25 PRIMER_LEFT_51_TM=62.827 PRIMER_INTERNAL_51_TM=59.556 PRIMER_LEFT_51_GC_PERCENT=68.421 PRIMER_INTERNAL_51_GC_PERCENT=56.000 PRIMER_INTERNAL_51_SELF_ANY_TH=8.63 PRIMER_LEFT_51_SELF_ANY_TH=4.39 PRIMER_INTERNAL_51_SELF_END_TH=0.00 PRIMER_LEFT_51_SELF_END_TH=0.00 PRIMER_LEFT_51_HAIRPIN_TH=45.50 PRIMER_INTERNAL_51_HAIRPIN_TH=32.68 PRIMER_LEFT_51_LIBRARY_MISPRIMING=9.00, HSATII Human satellite II DNA PRIMER_LEFT_51_END_STABILITY=4.7900 PRIMER_LEFT_51_TEMPLATE_MISPRIMING_TH=4.3247 PRIMER_INTERNAL_52_PENALTY=5.459011 PRIMER_INTERNAL_52_SEQUENCE=AGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_52=63,23 PRIMER_INTERNAL_52_TM=62.459 PRIMER_INTERNAL_52_GC_PERCENT=65.217 PRIMER_INTERNAL_52_SELF_ANY_TH=5.61 PRIMER_INTERNAL_52_SELF_END_TH=2.93 PRIMER_INTERNAL_52_HAIRPIN_TH=41.60 PRIMER_INTERNAL_53_PENALTY=5.514983 PRIMER_INTERNAL_53_SEQUENCE=TCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_53=73,24 PRIMER_INTERNAL_53_TM=58.485 PRIMER_INTERNAL_53_GC_PERCENT=54.167 PRIMER_INTERNAL_53_SELF_ANY_TH=0.30 PRIMER_INTERNAL_53_SELF_END_TH=0.00 PRIMER_INTERNAL_53_HAIRPIN_TH=41.28 PRIMER_INTERNAL_54_PENALTY=5.518553 PRIMER_INTERNAL_54_SEQUENCE=CGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_54=74,24 PRIMER_INTERNAL_54_TM=58.481 PRIMER_INTERNAL_54_GC_PERCENT=54.167 PRIMER_INTERNAL_54_SELF_ANY_TH=0.00 PRIMER_INTERNAL_54_SELF_END_TH=0.00 PRIMER_INTERNAL_54_HAIRPIN_TH=41.28 PRIMER_INTERNAL_55_PENALTY=5.523565 PRIMER_INTERNAL_55_SEQUENCE=CGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_55=74,23 PRIMER_INTERNAL_55_TM=57.476 PRIMER_INTERNAL_55_GC_PERCENT=56.522 PRIMER_INTERNAL_55_SELF_ANY_TH=0.00 PRIMER_INTERNAL_55_SELF_END_TH=0.00 PRIMER_INTERNAL_55_HAIRPIN_TH=41.28 PRIMER_INTERNAL_56_PENALTY=5.550131 PRIMER_INTERNAL_56_SEQUENCE=CACACACACACAGCACACAGGTA PRIMER_INTERNAL_56=38,23 PRIMER_INTERNAL_56_TM=57.450 PRIMER_INTERNAL_56_GC_PERCENT=52.174 PRIMER_INTERNAL_56_SELF_ANY_TH=0.00 PRIMER_INTERNAL_56_SELF_END_TH=0.00 PRIMER_INTERNAL_56_HAIRPIN_TH=0.00 PRIMER_INTERNAL_57_PENALTY=5.564155 PRIMER_INTERNAL_57_SEQUENCE=TCGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_57=73,25 PRIMER_INTERNAL_57_TM=59.436 PRIMER_INTERNAL_57_GC_PERCENT=52.000 PRIMER_INTERNAL_57_SELF_ANY_TH=0.30 PRIMER_INTERNAL_57_SELF_END_TH=0.00 PRIMER_INTERNAL_57_HAIRPIN_TH=41.28 PRIMER_INTERNAL_58_PENALTY=5.572672 PRIMER_INTERNAL_58_SEQUENCE=CAGCACACAGGTATTAGTGGGCC PRIMER_INTERNAL_58=48,23 PRIMER_INTERNAL_58_TM=57.427 PRIMER_INTERNAL_58_GC_PERCENT=56.522 PRIMER_INTERNAL_58_SELF_ANY_TH=8.63 PRIMER_INTERNAL_58_SELF_END_TH=0.00 PRIMER_INTERNAL_58_HAIRPIN_TH=32.68 PRIMER_INTERNAL_59_PENALTY=5.697915 PRIMER_INTERNAL_59_SEQUENCE=GTGGGCCATTCGATCCCGACCCA PRIMER_INTERNAL_59=64,23 PRIMER_INTERNAL_59_TM=62.698 PRIMER_INTERNAL_59_GC_PERCENT=65.217 PRIMER_INTERNAL_59_SELF_ANY_TH=8.58 PRIMER_INTERNAL_59_SELF_END_TH=6.40 PRIMER_INTERNAL_59_HAIRPIN_TH=42.67 PRIMER_INTERNAL_60_PENALTY=5.806699 PRIMER_INTERNAL_60_SEQUENCE=CCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_60=78,24 PRIMER_INTERNAL_60_TM=58.193 PRIMER_INTERNAL_60_GC_PERCENT=54.167 PRIMER_INTERNAL_60_SELF_ANY_TH=12.68 PRIMER_INTERNAL_60_SELF_END_TH=12.68 PRIMER_INTERNAL_60_HAIRPIN_TH=41.28 PRIMER_INTERNAL_61_PENALTY=5.806699 PRIMER_INTERNAL_61_SEQUENCE=TCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_61=77,24 PRIMER_INTERNAL_61_TM=58.193 PRIMER_INTERNAL_61_GC_PERCENT=54.167 PRIMER_INTERNAL_61_SELF_ANY_TH=5.17 PRIMER_INTERNAL_61_SELF_END_TH=5.17 PRIMER_INTERNAL_61_HAIRPIN_TH=41.28 PRIMER_INTERNAL_62_PENALTY=5.824076 PRIMER_INTERNAL_62_SEQUENCE=CCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_62=78,23 PRIMER_INTERNAL_62_TM=57.176 PRIMER_INTERNAL_62_GC_PERCENT=56.522 PRIMER_INTERNAL_62_SELF_ANY_TH=5.17 PRIMER_INTERNAL_62_SELF_END_TH=5.17 PRIMER_INTERNAL_62_HAIRPIN_TH=41.28 PRIMER_INTERNAL_63_PENALTY=5.846052 PRIMER_INTERNAL_63_SEQUENCE=TCCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_63=77,25 PRIMER_INTERNAL_63_TM=59.154 PRIMER_INTERNAL_63_GC_PERCENT=52.000 PRIMER_INTERNAL_63_SELF_ANY_TH=12.68 PRIMER_INTERNAL_63_SELF_END_TH=12.68 PRIMER_INTERNAL_63_HAIRPIN_TH=41.28 PRIMER_INTERNAL_64_PENALTY=5.892980 PRIMER_INTERNAL_64_SEQUENCE=TAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_64=62,24 PRIMER_INTERNAL_64_TM=61.893 PRIMER_INTERNAL_64_GC_PERCENT=62.500 PRIMER_INTERNAL_64_SELF_ANY_TH=5.61 PRIMER_INTERNAL_64_SELF_END_TH=2.93 PRIMER_INTERNAL_64_HAIRPIN_TH=41.60 PRIMER_INTERNAL_65_PENALTY=5.951461 PRIMER_INTERNAL_65_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_65=57,25 PRIMER_INTERNAL_65_TM=59.049 PRIMER_INTERNAL_65_GC_PERCENT=56.000 PRIMER_INTERNAL_65_SELF_ANY_TH=4.28 PRIMER_INTERNAL_65_SELF_END_TH=0.00 PRIMER_INTERNAL_65_HAIRPIN_TH=37.07 PRIMER_INTERNAL_66_PENALTY=5.980536 PRIMER_INTERNAL_66_SEQUENCE=CATTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_66=70,23 PRIMER_INTERNAL_66_TM=57.019 PRIMER_INTERNAL_66_GC_PERCENT=52.174 PRIMER_INTERNAL_66_SELF_ANY_TH=0.00 PRIMER_INTERNAL_66_SELF_END_TH=0.00 PRIMER_INTERNAL_66_HAIRPIN_TH=41.60 PRIMER_INTERNAL_67_PENALTY=6.009338 PRIMER_INTERNAL_67_SEQUENCE=CCATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_67=69,25 PRIMER_INTERNAL_67_TM=58.991 PRIMER_INTERNAL_67_GC_PERCENT=52.000 PRIMER_INTERNAL_67_SELF_ANY_TH=0.00 PRIMER_INTERNAL_67_SELF_END_TH=0.00 PRIMER_INTERNAL_67_HAIRPIN_TH=41.60 PRIMER_INTERNAL_68_PENALTY=6.015417 PRIMER_INTERNAL_68_SEQUENCE=ACACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_68=45,25 PRIMER_INTERNAL_68_TM=58.985 PRIMER_INTERNAL_68_GC_PERCENT=52.000 PRIMER_INTERNAL_68_SELF_ANY_TH=5.21 PRIMER_INTERNAL_68_SELF_END_TH=5.21 PRIMER_INTERNAL_68_HAIRPIN_TH=33.51 PRIMER_INTERNAL_69_PENALTY=6.046206 PRIMER_INTERNAL_69_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACC PRIMER_INTERNAL_69=59,26 PRIMER_INTERNAL_69_TM=59.954 PRIMER_INTERNAL_69_GC_PERCENT=53.846 PRIMER_INTERNAL_69_SELF_ANY_TH=5.61 PRIMER_INTERNAL_69_SELF_END_TH=0.00 PRIMER_INTERNAL_69_HAIRPIN_TH=41.60 PRIMER_INTERNAL_70_PENALTY=6.046206 PRIMER_INTERNAL_70_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_70=57,26 PRIMER_INTERNAL_70_TM=59.954 PRIMER_INTERNAL_70_GC_PERCENT=53.846 PRIMER_INTERNAL_70_SELF_ANY_TH=0.76 PRIMER_INTERNAL_70_SELF_END_TH=0.00 PRIMER_INTERNAL_70_HAIRPIN_TH=41.96 PRIMER_INTERNAL_71_PENALTY=6.046659 PRIMER_INTERNAL_71_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_71=56,26 PRIMER_INTERNAL_71_TM=59.953 PRIMER_INTERNAL_71_GC_PERCENT=53.846 PRIMER_INTERNAL_71_SELF_ANY_TH=4.31 PRIMER_INTERNAL_71_SELF_END_TH=0.00 PRIMER_INTERNAL_71_HAIRPIN_TH=37.07 PRIMER_INTERNAL_72_PENALTY=6.049301 PRIMER_INTERNAL_72_SEQUENCE=ACAGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_72=47,26 PRIMER_INTERNAL_72_TM=59.951 PRIMER_INTERNAL_72_GC_PERCENT=50.000 PRIMER_INTERNAL_72_SELF_ANY_TH=8.63 PRIMER_INTERNAL_72_SELF_END_TH=0.00 PRIMER_INTERNAL_72_HAIRPIN_TH=32.68 PRIMER_INTERNAL_73_PENALTY=6.095821 PRIMER_INTERNAL_73_SEQUENCE=GCACACAGGTATTAGTGGGCCATTCG PRIMER_INTERNAL_73=50,26 PRIMER_INTERNAL_73_TM=59.904 PRIMER_INTERNAL_73_GC_PERCENT=53.846 PRIMER_INTERNAL_73_SELF_ANY_TH=2.67 PRIMER_INTERNAL_73_SELF_END_TH=0.00 PRIMER_INTERNAL_73_HAIRPIN_TH=28.81 PRIMER_INTERNAL_74_PENALTY=6.114152 PRIMER_INTERNAL_74_SEQUENCE=AGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_74=49,24 PRIMER_INTERNAL_74_TM=57.886 PRIMER_INTERNAL_74_GC_PERCENT=50.000 PRIMER_INTERNAL_74_SELF_ANY_TH=8.63 PRIMER_INTERNAL_74_SELF_END_TH=0.00 PRIMER_INTERNAL_74_HAIRPIN_TH=32.68 PRIMER_INTERNAL_75_PENALTY=6.125318 PRIMER_INTERNAL_75_SEQUENCE=GCCATTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_75=68,25 PRIMER_INTERNAL_75_TM=61.125 PRIMER_INTERNAL_75_GC_PERCENT=56.000 PRIMER_INTERNAL_75_SELF_ANY_TH=0.00 PRIMER_INTERNAL_75_SELF_END_TH=0.00 PRIMER_INTERNAL_75_HAIRPIN_TH=41.60 PRIMER_INTERNAL_76_PENALTY=6.131527 PRIMER_INTERNAL_76_SEQUENCE=ACACACACACACACAGCACACAGGT PRIMER_INTERNAL_76=35,25 PRIMER_INTERNAL_76_TM=61.132 PRIMER_INTERNAL_76_GC_PERCENT=52.000 PRIMER_INTERNAL_76_SELF_ANY_TH=0.00 PRIMER_INTERNAL_76_SELF_END_TH=0.00 PRIMER_INTERNAL_76_HAIRPIN_TH=0.00 PRIMER_INTERNAL_77_PENALTY=6.186005 PRIMER_INTERNAL_77_SEQUENCE=ACACACACACACAGCACACAGGTAT PRIMER_INTERNAL_77=37,25 PRIMER_INTERNAL_77_TM=58.814 PRIMER_INTERNAL_77_GC_PERCENT=48.000 PRIMER_INTERNAL_77_SELF_ANY_TH=0.00 PRIMER_INTERNAL_77_SELF_END_TH=0.00 PRIMER_INTERNAL_77_HAIRPIN_TH=0.00 PRIMER_INTERNAL_78_PENALTY=6.195851 PRIMER_INTERNAL_78_SEQUENCE=CAGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_78=48,25 PRIMER_INTERNAL_78_TM=58.804 PRIMER_INTERNAL_78_GC_PERCENT=52.000 PRIMER_INTERNAL_78_SELF_ANY_TH=8.63 PRIMER_INTERNAL_78_SELF_END_TH=0.00 PRIMER_INTERNAL_78_HAIRPIN_TH=32.68 PRIMER_INTERNAL_79_PENALTY=6.203782 PRIMER_INTERNAL_79_SEQUENCE=CACACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_79=44,26 PRIMER_INTERNAL_79_TM=59.796 PRIMER_INTERNAL_79_GC_PERCENT=53.846 PRIMER_INTERNAL_79_SELF_ANY_TH=5.21 PRIMER_INTERNAL_79_SELF_END_TH=5.21 PRIMER_INTERNAL_79_HAIRPIN_TH=33.51 PRIMER_INTERNAL_80_PENALTY=6.214266 PRIMER_INTERNAL_80_SEQUENCE=CACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_80=46,24 PRIMER_INTERNAL_80_TM=57.786 PRIMER_INTERNAL_80_GC_PERCENT=54.167 PRIMER_INTERNAL_80_SELF_ANY_TH=5.21 PRIMER_INTERNAL_80_SELF_END_TH=5.21 PRIMER_INTERNAL_80_HAIRPIN_TH=33.51 PRIMER_INTERNAL_81_PENALTY=6.225896 PRIMER_INTERNAL_81_SEQUENCE=TTCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_81=72,25 PRIMER_INTERNAL_81_TM=58.774 PRIMER_INTERNAL_81_GC_PERCENT=52.000 PRIMER_INTERNAL_81_SELF_ANY_TH=0.00 PRIMER_INTERNAL_81_SELF_END_TH=0.00 PRIMER_INTERNAL_81_HAIRPIN_TH=41.60 PRIMER_INTERNAL_82_PENALTY=6.265114 PRIMER_INTERNAL_82_SEQUENCE=TGGGCCATTCGATCCCGACCCAAA PRIMER_INTERNAL_82=65,24 PRIMER_INTERNAL_82_TM=62.265 PRIMER_INTERNAL_82_GC_PERCENT=58.333 PRIMER_INTERNAL_82_SELF_ANY_TH=8.58 PRIMER_INTERNAL_82_SELF_END_TH=0.00 PRIMER_INTERNAL_82_HAIRPIN_TH=41.29 PRIMER_INTERNAL_83_PENALTY=6.318558 PRIMER_INTERNAL_83_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_83=72,26 PRIMER_INTERNAL_83_TM=59.681 PRIMER_INTERNAL_83_GC_PERCENT=50.000 PRIMER_INTERNAL_83_SELF_ANY_TH=0.00 PRIMER_INTERNAL_83_SELF_END_TH=0.00 PRIMER_INTERNAL_83_HAIRPIN_TH=41.60 PRIMER_INTERNAL_84_PENALTY=6.365515 PRIMER_INTERNAL_84_SEQUENCE=CACACACACACACAGCACACAGGTAT PRIMER_INTERNAL_84=36,26 PRIMER_INTERNAL_84_TM=59.634 PRIMER_INTERNAL_84_GC_PERCENT=50.000 PRIMER_INTERNAL_84_SELF_ANY_TH=0.00 PRIMER_INTERNAL_84_SELF_END_TH=0.00 PRIMER_INTERNAL_84_HAIRPIN_TH=0.00 PRIMER_INTERNAL_85_PENALTY=6.390437 PRIMER_INTERNAL_85_SEQUENCE=CACACACACACAGCACACAGGTAT PRIMER_INTERNAL_85=38,24 PRIMER_INTERNAL_85_TM=57.610 PRIMER_INTERNAL_85_GC_PERCENT=50.000 PRIMER_INTERNAL_85_SELF_ANY_TH=0.00 PRIMER_INTERNAL_85_SELF_END_TH=0.00 PRIMER_INTERNAL_85_HAIRPIN_TH=0.00 PRIMER_INTERNAL_86_PENALTY=6.635581 PRIMER_INTERNAL_86_SEQUENCE=GGGCCATTCGATCCCGACCCAAATC PRIMER_INTERNAL_86=66,25 PRIMER_INTERNAL_86_TM=61.636 PRIMER_INTERNAL_86_GC_PERCENT=60.000 PRIMER_INTERNAL_86_SELF_ANY_TH=2.16 PRIMER_INTERNAL_86_SELF_END_TH=0.00 PRIMER_INTERNAL_86_HAIRPIN_TH=41.60 PRIMER_INTERNAL_87_PENALTY=6.661393 PRIMER_INTERNAL_87_SEQUENCE=ACACACACACACACAGCACACAGGTA PRIMER_INTERNAL_87=35,26 PRIMER_INTERNAL_87_TM=60.661 PRIMER_INTERNAL_87_GC_PERCENT=50.000 PRIMER_INTERNAL_87_SELF_ANY_TH=0.00 PRIMER_INTERNAL_87_SELF_END_TH=0.00 PRIMER_INTERNAL_87_HAIRPIN_TH=0.00 PRIMER_INTERNAL_88_PENALTY=6.664685 PRIMER_INTERNAL_88_SEQUENCE=CACAGCACACAGGTATTAGTGGGCCA PRIMER_INTERNAL_88=46,26 PRIMER_INTERNAL_88_TM=60.665 PRIMER_INTERNAL_88_GC_PERCENT=53.846 PRIMER_INTERNAL_88_SELF_ANY_TH=8.63 PRIMER_INTERNAL_88_SELF_END_TH=1.50 PRIMER_INTERNAL_88_HAIRPIN_TH=32.68 PRIMER_INTERNAL_89_PENALTY=6.664922 PRIMER_INTERNAL_89_SEQUENCE=ACACAGCACACAGGTATTAGTGGGCC PRIMER_INTERNAL_89=45,26 PRIMER_INTERNAL_89_TM=60.665 PRIMER_INTERNAL_89_GC_PERCENT=53.846 PRIMER_INTERNAL_89_SELF_ANY_TH=8.63 PRIMER_INTERNAL_89_SELF_END_TH=0.00 PRIMER_INTERNAL_89_HAIRPIN_TH=32.68 PRIMER_INTERNAL_90_PENALTY=6.679783 PRIMER_INTERNAL_90_SEQUENCE=CCCGACCCAAATCGATAGCTACGAT PRIMER_INTERNAL_90=78,25 PRIMER_INTERNAL_90_TM=58.320 PRIMER_INTERNAL_90_GC_PERCENT=52.000 PRIMER_INTERNAL_90_SELF_ANY_TH=14.48 PRIMER_INTERNAL_90_SELF_END_TH=14.48 PRIMER_INTERNAL_90_HAIRPIN_TH=46.54 PRIMER_INTERNAL_91_PENALTY=6.679783 PRIMER_INTERNAL_91_SEQUENCE=ATCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_91=76,25 PRIMER_INTERNAL_91_TM=58.320 PRIMER_INTERNAL_91_GC_PERCENT=52.000 PRIMER_INTERNAL_91_SELF_ANY_TH=5.17 PRIMER_INTERNAL_91_SELF_END_TH=5.17 PRIMER_INTERNAL_91_HAIRPIN_TH=41.28 PRIMER_INTERNAL_92_PENALTY=6.695664 PRIMER_INTERNAL_92_SEQUENCE=TATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_92=59,24 PRIMER_INTERNAL_92_TM=57.304 PRIMER_INTERNAL_92_GC_PERCENT=50.000 PRIMER_INTERNAL_92_SELF_ANY_TH=0.00 PRIMER_INTERNAL_92_SELF_END_TH=0.00 PRIMER_INTERNAL_92_HAIRPIN_TH=41.96 PRIMER_INTERNAL_93_PENALTY=6.741797 PRIMER_INTERNAL_93_SEQUENCE=GTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_93=58,24 PRIMER_INTERNAL_93_TM=57.258 PRIMER_INTERNAL_93_GC_PERCENT=54.167 PRIMER_INTERNAL_93_SELF_ANY_TH=4.28 PRIMER_INTERNAL_93_SELF_END_TH=0.00 PRIMER_INTERNAL_93_HAIRPIN_TH=37.07 PRIMER_INTERNAL_94_PENALTY=6.754240 PRIMER_INTERNAL_94_SEQUENCE=TATTAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_94=59,25 PRIMER_INTERNAL_94_TM=58.246 PRIMER_INTERNAL_94_GC_PERCENT=52.000 PRIMER_INTERNAL_94_SELF_ANY_TH=3.57 PRIMER_INTERNAL_94_SELF_END_TH=3.02 PRIMER_INTERNAL_94_HAIRPIN_TH=41.60 PRIMER_INTERNAL_95_PENALTY=6.754240 PRIMER_INTERNAL_95_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_95=58,25 PRIMER_INTERNAL_95_TM=58.246 PRIMER_INTERNAL_95_GC_PERCENT=52.000 PRIMER_INTERNAL_95_SELF_ANY_TH=0.00 PRIMER_INTERNAL_95_SELF_END_TH=0.00 PRIMER_INTERNAL_95_HAIRPIN_TH=41.96 PRIMER_INTERNAL_96_PENALTY=6.756422 PRIMER_INTERNAL_96_SEQUENCE=TCCCGACCCAAATCGATAGCTACGAT PRIMER_INTERNAL_96=77,26 PRIMER_INTERNAL_96_TM=59.244 PRIMER_INTERNAL_96_GC_PERCENT=50.000 PRIMER_INTERNAL_96_SELF_ANY_TH=14.48 PRIMER_INTERNAL_96_SELF_END_TH=14.48 PRIMER_INTERNAL_96_HAIRPIN_TH=46.54 PRIMER_INTERNAL_97_PENALTY=6.756422 PRIMER_INTERNAL_97_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_97=76,26 PRIMER_INTERNAL_97_TM=59.244 PRIMER_INTERNAL_97_GC_PERCENT=50.000 PRIMER_INTERNAL_97_SELF_ANY_TH=12.68 PRIMER_INTERNAL_97_SELF_END_TH=12.68 PRIMER_INTERNAL_97_HAIRPIN_TH=41.28 PRIMER_INTERNAL_98_PENALTY=6.791532 PRIMER_INTERNAL_98_SEQUENCE=AGCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_98=49,25 PRIMER_INTERNAL_98_TM=58.208 PRIMER_INTERNAL_98_GC_PERCENT=48.000 PRIMER_INTERNAL_98_SELF_ANY_TH=8.63 PRIMER_INTERNAL_98_SELF_END_TH=0.00 PRIMER_INTERNAL_98_HAIRPIN_TH=32.68 PRIMER_INTERNAL_99_PENALTY=6.793369 PRIMER_INTERNAL_99_SEQUENCE=GCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_99=50,24 PRIMER_INTERNAL_99_TM=57.207 PRIMER_INTERNAL_99_GC_PERCENT=50.000 PRIMER_INTERNAL_99_SELF_ANY_TH=2.67 PRIMER_INTERNAL_99_SELF_END_TH=0.00 PRIMER_INTERNAL_99_HAIRPIN_TH=28.81 PRIMER_INTERNAL_100_PENALTY=6.804314 PRIMER_INTERNAL_100_SEQUENCE=CATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_100=70,24 PRIMER_INTERNAL_100_TM=57.196 PRIMER_INTERNAL_100_GC_PERCENT=50.000 PRIMER_INTERNAL_100_SELF_ANY_TH=0.00 PRIMER_INTERNAL_100_SELF_END_TH=0.00 PRIMER_INTERNAL_100_HAIRPIN_TH=41.60 PRIMER_INTERNAL_101_PENALTY=6.828710 PRIMER_INTERNAL_101_SEQUENCE=GTGGGCCATTCGATCCCGACCCAA PRIMER_INTERNAL_101=64,24 PRIMER_INTERNAL_101_TM=62.829 PRIMER_INTERNAL_101_GC_PERCENT=62.500 PRIMER_INTERNAL_101_SELF_ANY_TH=8.58 PRIMER_INTERNAL_101_SELF_END_TH=0.00 PRIMER_INTERNAL_101_HAIRPIN_TH=43.79 PRIMER_INTERNAL_102_PENALTY=6.841708 PRIMER_INTERNAL_102_SEQUENCE=CCCGACCCAAATCGATAGCTACGATG PRIMER_INTERNAL_102=78,26 PRIMER_INTERNAL_102_TM=59.158 PRIMER_INTERNAL_102_GC_PERCENT=53.846 PRIMER_INTERNAL_102_SELF_ANY_TH=5.23 PRIMER_INTERNAL_102_SELF_END_TH=0.00 PRIMER_INTERNAL_102_HAIRPIN_TH=43.02 PRIMER_INTERNAL_103_PENALTY=6.895596 PRIMER_INTERNAL_103_SEQUENCE=GGCCATTCGATCCCGACCCAAATCG PRIMER_INTERNAL_103=67,25 PRIMER_INTERNAL_103_TM=61.896 PRIMER_INTERNAL_103_GC_PERCENT=60.000 PRIMER_INTERNAL_103_SELF_ANY_TH=0.00 PRIMER_INTERNAL_103_SELF_END_TH=0.00 PRIMER_INTERNAL_103_HAIRPIN_TH=41.60 PRIMER_INTERNAL_104_PENALTY=6.905174 PRIMER_INTERNAL_104_SEQUENCE=CGATCCCGACCCAAATCGATAGCTA PRIMER_INTERNAL_104=74,25 PRIMER_INTERNAL_104_TM=58.095 PRIMER_INTERNAL_104_GC_PERCENT=52.000 PRIMER_INTERNAL_104_SELF_ANY_TH=0.00 PRIMER_INTERNAL_104_SELF_END_TH=0.00 PRIMER_INTERNAL_104_HAIRPIN_TH=41.28 PRIMER_INTERNAL_105_PENALTY=6.905408 PRIMER_INTERNAL_105_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_105=58,26 PRIMER_INTERNAL_105_TM=59.095 PRIMER_INTERNAL_105_GC_PERCENT=53.846 PRIMER_INTERNAL_105_SELF_ANY_TH=3.57 PRIMER_INTERNAL_105_SELF_END_TH=3.02 PRIMER_INTERNAL_105_HAIRPIN_TH=41.60 PRIMER_INTERNAL_106_PENALTY=6.917157 PRIMER_INTERNAL_106_SEQUENCE=ACACACACACACAGCACACAGGTATT PRIMER_INTERNAL_106=37,26 PRIMER_INTERNAL_106_TM=59.083 PRIMER_INTERNAL_106_GC_PERCENT=46.154 PRIMER_INTERNAL_106_SELF_ANY_TH=0.00 PRIMER_INTERNAL_106_SELF_END_TH=0.00 PRIMER_INTERNAL_106_HAIRPIN_TH=0.00 PRIMER_INTERNAL_107_PENALTY=6.924766 PRIMER_INTERNAL_107_SEQUENCE=CAGCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_107=48,26 PRIMER_INTERNAL_107_TM=59.075 PRIMER_INTERNAL_107_GC_PERCENT=50.000 PRIMER_INTERNAL_107_SELF_ANY_TH=8.63 PRIMER_INTERNAL_107_SELF_END_TH=0.00 PRIMER_INTERNAL_107_HAIRPIN_TH=32.68 PRIMER_INTERNAL_108_PENALTY=6.956454 PRIMER_INTERNAL_108_SEQUENCE=AGACATGACACTGACGATGCAGAC PRIMER_INTERNAL_108=10,24 PRIMER_INTERNAL_108_TM=57.044 PRIMER_INTERNAL_108_GC_PERCENT=50.000 PRIMER_INTERNAL_108_SELF_ANY_TH=1.44 PRIMER_INTERNAL_108_SELF_END_TH=0.00 PRIMER_INTERNAL_108_HAIRPIN_TH=0.00 PRIMER_INTERNAL_109_PENALTY=6.975446 PRIMER_INTERNAL_109_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTA PRIMER_INTERNAL_109=73,26 PRIMER_INTERNAL_109_TM=59.025 PRIMER_INTERNAL_109_GC_PERCENT=50.000 PRIMER_INTERNAL_109_SELF_ANY_TH=0.30 PRIMER_INTERNAL_109_SELF_END_TH=0.00 PRIMER_INTERNAL_109_HAIRPIN_TH=41.28 PRIMER_INTERNAL_110_PENALTY=7.015932 PRIMER_INTERNAL_110_SEQUENCE=CCCGACCCAAATCGATAGCTACGATGA PRIMER_INTERNAL_110=78,27 PRIMER_INTERNAL_110_TM=60.016 PRIMER_INTERNAL_110_GC_PERCENT=51.852 PRIMER_INTERNAL_110_SELF_ANY_TH=7.53 PRIMER_INTERNAL_110_SELF_END_TH=0.00 PRIMER_INTERNAL_110_HAIRPIN_TH=43.02 PRIMER_INTERNAL_111_PENALTY=7.015932 PRIMER_INTERNAL_111_SEQUENCE=TCCCGACCCAAATCGATAGCTACGATG PRIMER_INTERNAL_111=77,27 PRIMER_INTERNAL_111_TM=60.016 PRIMER_INTERNAL_111_GC_PERCENT=51.852 PRIMER_INTERNAL_111_SELF_ANY_TH=5.23 PRIMER_INTERNAL_111_SELF_END_TH=0.00 PRIMER_INTERNAL_111_HAIRPIN_TH=43.02 PRIMER_INTERNAL_112_PENALTY=7.051167 PRIMER_INTERNAL_112_SEQUENCE=TTAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_112=61,25 PRIMER_INTERNAL_112_TM=62.051 PRIMER_INTERNAL_112_GC_PERCENT=60.000 PRIMER_INTERNAL_112_SELF_ANY_TH=5.61 PRIMER_INTERNAL_112_SELF_END_TH=2.93 PRIMER_INTERNAL_112_HAIRPIN_TH=41.60 PRIMER_INTERNAL_113_PENALTY=7.056335 PRIMER_INTERNAL_113_SEQUENCE=GATCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_113=75,26 PRIMER_INTERNAL_113_TM=58.944 PRIMER_INTERNAL_113_GC_PERCENT=53.846 PRIMER_INTERNAL_113_SELF_ANY_TH=5.17 PRIMER_INTERNAL_113_SELF_END_TH=5.17 PRIMER_INTERNAL_113_HAIRPIN_TH=41.28 PRIMER_INTERNAL_114_PENALTY=7.056335 PRIMER_INTERNAL_114_SEQUENCE=CGATCCCGACCCAAATCGATAGCTAC PRIMER_INTERNAL_114=74,26 PRIMER_INTERNAL_114_TM=58.944 PRIMER_INTERNAL_114_GC_PERCENT=53.846 PRIMER_INTERNAL_114_SELF_ANY_TH=0.00 PRIMER_INTERNAL_114_SELF_END_TH=0.00 PRIMER_INTERNAL_114_HAIRPIN_TH=41.28 PRIMER_INTERNAL_115_PENALTY=7.066919 PRIMER_INTERNAL_115_SEQUENCE=CACACACACACAGCACACAGGTATT PRIMER_INTERNAL_115=38,25 PRIMER_INTERNAL_115_TM=57.933 PRIMER_INTERNAL_115_GC_PERCENT=48.000 PRIMER_INTERNAL_115_SELF_ANY_TH=0.00 PRIMER_INTERNAL_115_SELF_END_TH=0.00 PRIMER_INTERNAL_115_HAIRPIN_TH=0.00 PRIMER_INTERNAL_116_PENALTY=7.070364 PRIMER_INTERNAL_116_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCC PRIMER_INTERNAL_116=56,25 PRIMER_INTERNAL_116_TM=57.930 PRIMER_INTERNAL_116_GC_PERCENT=52.000 PRIMER_INTERNAL_116_SELF_ANY_TH=4.31 PRIMER_INTERNAL_116_SELF_END_TH=0.37 PRIMER_INTERNAL_116_HAIRPIN_TH=0.00 PRIMER_INTERNAL_117_PENALTY=7.079469 PRIMER_INTERNAL_117_SEQUENCE=GCACACAGGTATTAGTGGGCCATTC PRIMER_INTERNAL_117=50,25 PRIMER_INTERNAL_117_TM=57.921 PRIMER_INTERNAL_117_GC_PERCENT=52.000 PRIMER_INTERNAL_117_SELF_ANY_TH=2.67 PRIMER_INTERNAL_117_SELF_END_TH=0.00 PRIMER_INTERNAL_117_HAIRPIN_TH=28.81 PRIMER_INTERNAL_118_PENALTY=7.094172 PRIMER_INTERNAL_118_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCCC PRIMER_INTERNAL_118=54,27 PRIMER_INTERNAL_118_TM=59.906 PRIMER_INTERNAL_118_GC_PERCENT=51.852 PRIMER_INTERNAL_118_SELF_ANY_TH=4.31 PRIMER_INTERNAL_118_SELF_END_TH=0.37 PRIMER_INTERNAL_118_HAIRPIN_TH=0.00 PRIMER_INTERNAL_119_PENALTY=7.124042 PRIMER_INTERNAL_119_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_119=71,26 PRIMER_INTERNAL_119_TM=58.876 PRIMER_INTERNAL_119_GC_PERCENT=50.000 PRIMER_INTERNAL_119_SELF_ANY_TH=0.00 PRIMER_INTERNAL_119_SELF_END_TH=0.00 PRIMER_INTERNAL_119_HAIRPIN_TH=41.60 PRIMER_INTERNAL_120_PENALTY=7.139284 PRIMER_INTERNAL_120_SEQUENCE=CACACACACACACAGCACACAGGTATT PRIMER_INTERNAL_120=36,27 PRIMER_INTERNAL_120_TM=59.861 PRIMER_INTERNAL_120_GC_PERCENT=48.148 PRIMER_INTERNAL_120_SELF_ANY_TH=0.00 PRIMER_INTERNAL_120_SELF_END_TH=0.00 PRIMER_INTERNAL_120_HAIRPIN_TH=0.00 PRIMER_INTERNAL_121_PENALTY=7.145199 PRIMER_INTERNAL_121_SEQUENCE=GCCATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_121=68,26 PRIMER_INTERNAL_121_TM=61.145 PRIMER_INTERNAL_121_GC_PERCENT=53.846 PRIMER_INTERNAL_121_SELF_ANY_TH=0.00 PRIMER_INTERNAL_121_SELF_END_TH=0.00 PRIMER_INTERNAL_121_HAIRPIN_TH=41.60 PRIMER_INTERNAL_122_PENALTY=7.145368 PRIMER_INTERNAL_122_SEQUENCE=AGCACACAGGTATTAGTGGGCCATTC PRIMER_INTERNAL_122=49,26 PRIMER_INTERNAL_122_TM=58.855 PRIMER_INTERNAL_122_GC_PERCENT=50.000 PRIMER_INTERNAL_122_SELF_ANY_TH=8.63 PRIMER_INTERNAL_122_SELF_END_TH=0.00 PRIMER_INTERNAL_122_HAIRPIN_TH=32.68 PRIMER_INTERNAL_123_PENALTY=7.171756 PRIMER_INTERNAL_123_SEQUENCE=ACAGCACACAGGTATTAGTGGGCCATT PRIMER_INTERNAL_123=47,27 PRIMER_INTERNAL_123_TM=60.172 PRIMER_INTERNAL_123_GC_PERCENT=48.148 PRIMER_INTERNAL_123_SELF_ANY_TH=8.63 PRIMER_INTERNAL_123_SELF_END_TH=0.00 PRIMER_INTERNAL_123_HAIRPIN_TH=32.68 PRIMER_INTERNAL_124_PENALTY=7.192525 PRIMER_INTERNAL_124_SEQUENCE=GATCCCGACCCAAATCGATAGCTACGA PRIMER_INTERNAL_124=75,27 PRIMER_INTERNAL_124_TM=59.807 PRIMER_INTERNAL_124_GC_PERCENT=51.852 PRIMER_INTERNAL_124_SELF_ANY_TH=12.68 PRIMER_INTERNAL_124_SELF_END_TH=12.68 PRIMER_INTERNAL_124_HAIRPIN_TH=41.28 PRIMER_INTERNAL_125_PENALTY=7.192525 PRIMER_INTERNAL_125_SEQUENCE=TCGATCCCGACCCAAATCGATAGCTAC PRIMER_INTERNAL_125=73,27 PRIMER_INTERNAL_125_TM=59.807 PRIMER_INTERNAL_125_GC_PERCENT=51.852 PRIMER_INTERNAL_125_SELF_ANY_TH=0.30 PRIMER_INTERNAL_125_SELF_END_TH=0.00 PRIMER_INTERNAL_125_HAIRPIN_TH=41.28 PRIMER_INTERNAL_126_PENALTY=7.193259 PRIMER_INTERNAL_126_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCC PRIMER_INTERNAL_126=55,26 PRIMER_INTERNAL_126_TM=58.807 PRIMER_INTERNAL_126_GC_PERCENT=53.846 PRIMER_INTERNAL_126_SELF_ANY_TH=4.31 PRIMER_INTERNAL_126_SELF_END_TH=0.37 PRIMER_INTERNAL_126_HAIRPIN_TH=0.00 PRIMER_INTERNAL_127_PENALTY=7.239217 PRIMER_INTERNAL_127_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGA PRIMER_INTERNAL_127=52,25 PRIMER_INTERNAL_127_TM=57.761 PRIMER_INTERNAL_127_GC_PERCENT=48.000 PRIMER_INTERNAL_127_SELF_ANY_TH=0.00 PRIMER_INTERNAL_127_SELF_END_TH=0.00 PRIMER_INTERNAL_127_HAIRPIN_TH=0.00 PRIMER_INTERNAL_128_PENALTY=7.243997 PRIMER_INTERNAL_128_SEQUENCE=TGGGCCATTCGATCCCGACCCAAAT PRIMER_INTERNAL_128=65,25 PRIMER_INTERNAL_128_TM=62.244 PRIMER_INTERNAL_128_GC_PERCENT=56.000 PRIMER_INTERNAL_128_SELF_ANY_TH=8.58 PRIMER_INTERNAL_128_SELF_END_TH=0.00 PRIMER_INTERNAL_128_HAIRPIN_TH=41.29 PRIMER_INTERNAL_129_PENALTY=7.246964 PRIMER_INTERNAL_129_SEQUENCE=ACACACAGCACACAGGTATTAGTGGG PRIMER_INTERNAL_129=43,26 PRIMER_INTERNAL_129_TM=58.753 PRIMER_INTERNAL_129_GC_PERCENT=50.000 PRIMER_INTERNAL_129_SELF_ANY_TH=0.00 PRIMER_INTERNAL_129_SELF_END_TH=0.00 PRIMER_INTERNAL_129_HAIRPIN_TH=0.00 PRIMER_INTERNAL_130_PENALTY=7.250024 PRIMER_INTERNAL_130_SEQUENCE=ATTCGATCCCGACCCAAATCGATAGCT PRIMER_INTERNAL_130=71,27 PRIMER_INTERNAL_130_TM=59.750 PRIMER_INTERNAL_130_GC_PERCENT=48.148 PRIMER_INTERNAL_130_SELF_ANY_TH=0.00 PRIMER_INTERNAL_130_SELF_END_TH=0.00 PRIMER_INTERNAL_130_HAIRPIN_TH=41.60 PRIMER_INTERNAL_131_PENALTY=7.293083 PRIMER_INTERNAL_131_SEQUENCE=CACACAGGTATTAGTGGGCCATTCG PRIMER_INTERNAL_131=51,25 PRIMER_INTERNAL_131_TM=57.707 PRIMER_INTERNAL_131_GC_PERCENT=52.000 PRIMER_INTERNAL_131_SELF_ANY_TH=0.00 PRIMER_INTERNAL_131_SELF_END_TH=0.00 PRIMER_INTERNAL_131_HAIRPIN_TH=0.00 PRIMER_INTERNAL_132_PENALTY=7.342607 PRIMER_INTERNAL_132_SEQUENCE=CATTCGATCCCGACCCAAATCGATAGC PRIMER_INTERNAL_132=70,27 PRIMER_INTERNAL_132_TM=59.657 PRIMER_INTERNAL_132_GC_PERCENT=51.852 PRIMER_INTERNAL_132_SELF_ANY_TH=0.00 PRIMER_INTERNAL_132_SELF_END_TH=0.00 PRIMER_INTERNAL_132_HAIRPIN_TH=41.60 PRIMER_INTERNAL_133_PENALTY=7.350773 PRIMER_INTERNAL_133_SEQUENCE=CAGCACACAGGTATTAGTGGGCCATTC PRIMER_INTERNAL_133=48,27 PRIMER_INTERNAL_133_TM=59.649 PRIMER_INTERNAL_133_GC_PERCENT=51.852 PRIMER_INTERNAL_133_SELF_ANY_TH=8.63 PRIMER_INTERNAL_133_SELF_END_TH=0.00 PRIMER_INTERNAL_133_HAIRPIN_TH=32.68 PRIMER_INTERNAL_134_PENALTY=7.354919 PRIMER_INTERNAL_134_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGA PRIMER_INTERNAL_134=51,26 PRIMER_INTERNAL_134_TM=58.645 PRIMER_INTERNAL_134_GC_PERCENT=50.000 PRIMER_INTERNAL_134_SELF_ANY_TH=0.00 PRIMER_INTERNAL_134_SELF_END_TH=0.00 PRIMER_INTERNAL_134_HAIRPIN_TH=0.00 PRIMER_INTERNAL_135_PENALTY=7.401440 PRIMER_INTERNAL_135_SEQUENCE=CCATTCGATCCCGACCCAAATCGATA PRIMER_INTERNAL_135=69,26 PRIMER_INTERNAL_135_TM=58.599 PRIMER_INTERNAL_135_GC_PERCENT=50.000 PRIMER_INTERNAL_135_SELF_ANY_TH=0.00 PRIMER_INTERNAL_135_SELF_END_TH=0.00 PRIMER_INTERNAL_135_HAIRPIN_TH=41.60 PRIMER_INTERNAL_136_PENALTY=7.408271 PRIMER_INTERNAL_136_SEQUENCE=CACACAGCACACAGGTATTAGTGGG PRIMER_INTERNAL_136=44,25 PRIMER_INTERNAL_136_TM=57.592 PRIMER_INTERNAL_136_GC_PERCENT=52.000 PRIMER_INTERNAL_136_SELF_ANY_TH=0.00 PRIMER_INTERNAL_136_SELF_END_TH=0.00 PRIMER_INTERNAL_136_HAIRPIN_TH=0.00 PRIMER_INTERNAL_137_PENALTY=7.451841 PRIMER_INTERNAL_137_SEQUENCE=CACACACAGCACACAGGTATTAGTGGG PRIMER_INTERNAL_137=42,27 PRIMER_INTERNAL_137_TM=59.548 PRIMER_INTERNAL_137_GC_PERCENT=51.852 PRIMER_INTERNAL_137_SELF_ANY_TH=0.00 PRIMER_INTERNAL_137_SELF_END_TH=0.00 PRIMER_INTERNAL_137_HAIRPIN_TH=0.00 PRIMER_INTERNAL_138_PENALTY=7.534114 PRIMER_INTERNAL_138_SEQUENCE=CCGACCCAAATCGATAGCTACGATG PRIMER_INTERNAL_138=79,25 PRIMER_INTERNAL_138_TM=57.466 PRIMER_INTERNAL_138_GC_PERCENT=52.000 PRIMER_INTERNAL_138_SELF_ANY_TH=5.23 PRIMER_INTERNAL_138_SELF_END_TH=0.00 PRIMER_INTERNAL_138_HAIRPIN_TH=43.02 PRIMER_INTERNAL_139_PENALTY=7.537719 PRIMER_INTERNAL_139_SEQUENCE=CGACCCAAATCGATAGCTACGATGACG PRIMER_INTERNAL_139=80,27 PRIMER_INTERNAL_139_TM=59.462 PRIMER_INTERNAL_139_GC_PERCENT=51.852 PRIMER_INTERNAL_139_SELF_ANY_TH=6.52 PRIMER_INTERNAL_139_SELF_END_TH=4.23 PRIMER_INTERNAL_139_HAIRPIN_TH=43.02 PRIMER_INTERNAL_140_PENALTY=7.602591 PRIMER_INTERNAL_140_SEQUENCE=CCGACCCAAATCGATAGCTACGATGA PRIMER_INTERNAL_140=79,26 PRIMER_INTERNAL_140_TM=58.397 PRIMER_INTERNAL_140_GC_PERCENT=50.000 PRIMER_INTERNAL_140_SELF_ANY_TH=7.53 PRIMER_INTERNAL_140_SELF_END_TH=0.00 PRIMER_INTERNAL_140_HAIRPIN_TH=43.02 PRIMER_INTERNAL_141_PENALTY=7.672898 PRIMER_INTERNAL_141_SEQUENCE=ATCCCGACCCAAATCGATAGCTACGAT PRIMER_INTERNAL_141=76,27 PRIMER_INTERNAL_141_TM=59.327 PRIMER_INTERNAL_141_GC_PERCENT=48.148 PRIMER_INTERNAL_141_SELF_ANY_TH=14.48 PRIMER_INTERNAL_141_SELF_END_TH=14.48 PRIMER_INTERNAL_141_HAIRPIN_TH=46.54 PRIMER_INTERNAL_142_PENALTY=7.693260 PRIMER_INTERNAL_142_SEQUENCE=GCCATTCGATCCCGACCCAAATCGATA PRIMER_INTERNAL_142=68,27 PRIMER_INTERNAL_142_TM=60.693 PRIMER_INTERNAL_142_GC_PERCENT=51.852 PRIMER_INTERNAL_142_SELF_ANY_TH=0.00 PRIMER_INTERNAL_142_SELF_END_TH=0.00 PRIMER_INTERNAL_142_HAIRPIN_TH=41.60 PRIMER_INTERNAL_143_PENALTY=7.693499 PRIMER_INTERNAL_143_SEQUENCE=GTATTAGTGGGCCATTCGATCCCGACC PRIMER_INTERNAL_143=58,27 PRIMER_INTERNAL_143_TM=60.693 PRIMER_INTERNAL_143_GC_PERCENT=55.556 PRIMER_INTERNAL_143_SELF_ANY_TH=5.61 PRIMER_INTERNAL_143_SELF_END_TH=0.00 PRIMER_INTERNAL_143_HAIRPIN_TH=41.60 PRIMER_INTERNAL_144_PENALTY=7.693499 PRIMER_INTERNAL_144_SEQUENCE=GGTATTAGTGGGCCATTCGATCCCGAC PRIMER_INTERNAL_144=57,27 PRIMER_INTERNAL_144_TM=60.693 PRIMER_INTERNAL_144_GC_PERCENT=55.556 PRIMER_INTERNAL_144_SELF_ANY_TH=3.57 PRIMER_INTERNAL_144_SELF_END_TH=3.02 PRIMER_INTERNAL_144_HAIRPIN_TH=41.60 PRIMER_INTERNAL_145_PENALTY=7.694218 PRIMER_INTERNAL_145_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCCCG PRIMER_INTERNAL_145=55,27 PRIMER_INTERNAL_145_TM=60.694 PRIMER_INTERNAL_145_GC_PERCENT=55.556 PRIMER_INTERNAL_145_SELF_ANY_TH=4.31 PRIMER_INTERNAL_145_SELF_END_TH=0.00 PRIMER_INTERNAL_145_HAIRPIN_TH=37.07 PRIMER_INTERNAL_146_PENALTY=7.696389 PRIMER_INTERNAL_146_SEQUENCE=ACACACACACACACAGCACACAGGTAT PRIMER_INTERNAL_146=35,27 PRIMER_INTERNAL_146_TM=60.696 PRIMER_INTERNAL_146_GC_PERCENT=48.148 PRIMER_INTERNAL_146_SELF_ANY_TH=0.00 PRIMER_INTERNAL_146_SELF_END_TH=0.00 PRIMER_INTERNAL_146_HAIRPIN_TH=0.00 PRIMER_INTERNAL_147_PENALTY=7.699801 PRIMER_INTERNAL_147_SEQUENCE=CACAGCACACAGGTATTAGTGGGCCAT PRIMER_INTERNAL_147=46,27 PRIMER_INTERNAL_147_TM=60.700 PRIMER_INTERNAL_147_GC_PERCENT=51.852 PRIMER_INTERNAL_147_SELF_ANY_TH=8.63 PRIMER_INTERNAL_147_SELF_END_TH=0.00 PRIMER_INTERNAL_147_HAIRPIN_TH=32.68 PRIMER_INTERNAL_148_PENALTY=7.723400 PRIMER_INTERNAL_148_SEQUENCE=TTCGATCCCGACCCAAATCGATAGCTA PRIMER_INTERNAL_148=72,27 PRIMER_INTERNAL_148_TM=59.277 PRIMER_INTERNAL_148_GC_PERCENT=48.148 PRIMER_INTERNAL_148_SELF_ANY_TH=0.00 PRIMER_INTERNAL_148_SELF_END_TH=0.00 PRIMER_INTERNAL_148_HAIRPIN_TH=41.60 PRIMER_INTERNAL_149_PENALTY=7.748120 PRIMER_INTERNAL_149_SEQUENCE=GCACACAGGTATTAGTGGGCCATTCGA PRIMER_INTERNAL_149=50,27 PRIMER_INTERNAL_149_TM=60.748 PRIMER_INTERNAL_149_GC_PERCENT=51.852 PRIMER_INTERNAL_149_SELF_ANY_TH=2.67 PRIMER_INTERNAL_149_SELF_END_TH=0.00 PRIMER_INTERNAL_149_HAIRPIN_TH=28.81 PRIMER_INTERNAL_150_PENALTY=7.749187 PRIMER_INTERNAL_150_SEQUENCE=AGCACACAGGTATTAGTGGGCCATTCG PRIMER_INTERNAL_150=49,27 PRIMER_INTERNAL_150_TM=60.749 PRIMER_INTERNAL_150_GC_PERCENT=51.852 PRIMER_INTERNAL_150_SELF_ANY_TH=8.63 PRIMER_INTERNAL_150_SELF_END_TH=0.00 PRIMER_INTERNAL_150_HAIRPIN_TH=32.68 PRIMER_INTERNAL_151_PENALTY=7.753661 PRIMER_INTERNAL_151_SEQUENCE=ACAGGTATTAGTGGGCCATTCGATCC PRIMER_INTERNAL_151=54,26 PRIMER_INTERNAL_151_TM=58.246 PRIMER_INTERNAL_151_GC_PERCENT=50.000 PRIMER_INTERNAL_151_SELF_ANY_TH=2.01 PRIMER_INTERNAL_151_SELF_END_TH=1.68 PRIMER_INTERNAL_151_HAIRPIN_TH=0.00 PRIMER_INTERNAL_152_PENALTY=7.785822 PRIMER_INTERNAL_152_SEQUENCE=CGATCCCGACCCAAATCGATAGCTACG PRIMER_INTERNAL_152=74,27 PRIMER_INTERNAL_152_TM=60.786 PRIMER_INTERNAL_152_GC_PERCENT=55.556 PRIMER_INTERNAL_152_SELF_ANY_TH=5.17 PRIMER_INTERNAL_152_SELF_END_TH=5.17 PRIMER_INTERNAL_152_HAIRPIN_TH=41.28 PRIMER_INTERNAL_153_PENALTY=7.802259 PRIMER_INTERNAL_153_SEQUENCE=CCGACCCAAATCGATAGCTACGATGAC PRIMER_INTERNAL_153=79,27 PRIMER_INTERNAL_153_TM=59.198 PRIMER_INTERNAL_153_GC_PERCENT=51.852 PRIMER_INTERNAL_153_SELF_ANY_TH=5.43 PRIMER_INTERNAL_153_SELF_END_TH=0.00 PRIMER_INTERNAL_153_HAIRPIN_TH=43.02 PRIMER_INTERNAL_154_PENALTY=7.812760 PRIMER_INTERNAL_154_SEQUENCE=AGGTATTAGTGGGCCATTCGATCCCGA PRIMER_INTERNAL_154=56,27 PRIMER_INTERNAL_154_TM=60.813 PRIMER_INTERNAL_154_GC_PERCENT=51.852 PRIMER_INTERNAL_154_SELF_ANY_TH=4.31 PRIMER_INTERNAL_154_SELF_END_TH=0.00 PRIMER_INTERNAL_154_HAIRPIN_TH=41.96 PRIMER_INTERNAL_155_PENALTY=7.812835 PRIMER_INTERNAL_155_SEQUENCE=CCATTCGATCCCGACCCAAATCGATAG PRIMER_INTERNAL_155=69,27 PRIMER_INTERNAL_155_TM=59.187 PRIMER_INTERNAL_155_GC_PERCENT=51.852 PRIMER_INTERNAL_155_SELF_ANY_TH=0.00 PRIMER_INTERNAL_155_SELF_END_TH=0.00 PRIMER_INTERNAL_155_HAIRPIN_TH=41.60 PRIMER_INTERNAL_156_PENALTY=7.853399 PRIMER_INTERNAL_156_SEQUENCE=ACACACAGCACACAGGTATTAGTGGGC PRIMER_INTERNAL_156=43,27 PRIMER_INTERNAL_156_TM=60.853 PRIMER_INTERNAL_156_GC_PERCENT=51.852 PRIMER_INTERNAL_156_SELF_ANY_TH=5.21 PRIMER_INTERNAL_156_SELF_END_TH=5.21 PRIMER_INTERNAL_156_HAIRPIN_TH=33.51 PRIMER_INTERNAL_157_PENALTY=7.928092 PRIMER_INTERNAL_157_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATCC PRIMER_INTERNAL_157=53,27 PRIMER_INTERNAL_157_TM=59.072 PRIMER_INTERNAL_157_GC_PERCENT=51.852 PRIMER_INTERNAL_157_SELF_ANY_TH=2.01 PRIMER_INTERNAL_157_SELF_END_TH=1.68 PRIMER_INTERNAL_157_HAIRPIN_TH=0.00 PRIMER_INTERNAL_158_PENALTY=7.938713 PRIMER_INTERNAL_158_SEQUENCE=CAGGTATTAGTGGGCCATTCGATCC PRIMER_INTERNAL_158=55,25 PRIMER_INTERNAL_158_TM=57.061 PRIMER_INTERNAL_158_GC_PERCENT=52.000 PRIMER_INTERNAL_158_SELF_ANY_TH=2.01 PRIMER_INTERNAL_158_SELF_END_TH=1.68 PRIMER_INTERNAL_158_HAIRPIN_TH=0.00 PRIMER_INTERNAL_159_PENALTY=7.949148 PRIMER_INTERNAL_159_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAA PRIMER_INTERNAL_159=64,25 PRIMER_INTERNAL_159_TM=62.949 PRIMER_INTERNAL_159_GC_PERCENT=60.000 PRIMER_INTERNAL_159_SELF_ANY_TH=8.58 PRIMER_INTERNAL_159_SELF_END_TH=0.00 PRIMER_INTERNAL_159_HAIRPIN_TH=43.79 PRIMER_INTERNAL_160_PENALTY=7.979746 PRIMER_INTERNAL_160_SEQUENCE=ACACACACAGCACACAGGTATTAGTGG PRIMER_INTERNAL_160=41,27 PRIMER_INTERNAL_160_TM=59.020 PRIMER_INTERNAL_160_GC_PERCENT=48.148 PRIMER_INTERNAL_160_SELF_ANY_TH=0.00 PRIMER_INTERNAL_160_SELF_END_TH=0.00 PRIMER_INTERNAL_160_HAIRPIN_TH=0.00 PRIMER_INTERNAL_161_PENALTY=7.992102 PRIMER_INTERNAL_161_SEQUENCE=TAGTGGGCCATTCGATCCCGACCCA PRIMER_INTERNAL_161=62,25 PRIMER_INTERNAL_161_TM=62.992 PRIMER_INTERNAL_161_GC_PERCENT=60.000 PRIMER_INTERNAL_161_SELF_ANY_TH=8.58 PRIMER_INTERNAL_161_SELF_END_TH=6.40 PRIMER_INTERNAL_161_HAIRPIN_TH=42.67 PRIMER_INTERNAL_162_PENALTY=7.993148 PRIMER_INTERNAL_162_SEQUENCE=ACACACAGCACACAGGTATTAGTGG PRIMER_INTERNAL_162=43,25 PRIMER_INTERNAL_162_TM=57.007 PRIMER_INTERNAL_162_GC_PERCENT=48.000 PRIMER_INTERNAL_162_SELF_ANY_TH=0.00 PRIMER_INTERNAL_162_SELF_END_TH=0.00 PRIMER_INTERNAL_162_HAIRPIN_TH=0.00 PRIMER_INTERNAL_163_PENALTY=8.038590 PRIMER_INTERNAL_163_SEQUENCE=ATTAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_163=60,26 PRIMER_INTERNAL_163_TM=62.039 PRIMER_INTERNAL_163_GC_PERCENT=57.692 PRIMER_INTERNAL_163_SELF_ANY_TH=5.61 PRIMER_INTERNAL_163_SELF_END_TH=2.93 PRIMER_INTERNAL_163_HAIRPIN_TH=41.60 PRIMER_INTERNAL_164_PENALTY=8.084641 PRIMER_INTERNAL_164_SEQUENCE=CACACACAGCACACAGGTATTAGTGG PRIMER_INTERNAL_164=42,26 PRIMER_INTERNAL_164_TM=57.915 PRIMER_INTERNAL_164_GC_PERCENT=50.000 PRIMER_INTERNAL_164_SELF_ANY_TH=0.00 PRIMER_INTERNAL_164_SELF_END_TH=0.00 PRIMER_INTERNAL_164_HAIRPIN_TH=0.00 PRIMER_INTERNAL_165_PENALTY=8.098597 PRIMER_INTERNAL_165_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGAT PRIMER_INTERNAL_165=52,26 PRIMER_INTERNAL_165_TM=57.901 PRIMER_INTERNAL_165_GC_PERCENT=46.154 PRIMER_INTERNAL_165_SELF_ANY_TH=0.00 PRIMER_INTERNAL_165_SELF_END_TH=0.00 PRIMER_INTERNAL_165_HAIRPIN_TH=0.00 PRIMER_INTERNAL_166_PENALTY=8.106799 PRIMER_INTERNAL_166_SEQUENCE=TCAGTAGACATGACACTGACGATGCA PRIMER_INTERNAL_166=5,26 PRIMER_INTERNAL_166_TM=57.893 PRIMER_INTERNAL_166_GC_PERCENT=46.154 PRIMER_INTERNAL_166_SELF_ANY_TH=16.57 PRIMER_INTERNAL_166_SELF_END_TH=0.76 PRIMER_INTERNAL_166_HAIRPIN_TH=0.00 PRIMER_INTERNAL_167_PENALTY=8.251191 PRIMER_INTERNAL_167_SEQUENCE=CACACAGGTATTAGTGGGCCATTCGAT PRIMER_INTERNAL_167=51,27 PRIMER_INTERNAL_167_TM=58.749 PRIMER_INTERNAL_167_GC_PERCENT=48.148 PRIMER_INTERNAL_167_SELF_ANY_TH=0.00 PRIMER_INTERNAL_167_SELF_END_TH=0.00 PRIMER_INTERNAL_167_HAIRPIN_TH=0.00 PRIMER_INTERNAL_168_PENALTY=8.275978 PRIMER_INTERNAL_168_SEQUENCE=GTCAGTAGACATGACACTGACGATGCA PRIMER_INTERNAL_168=4,27 PRIMER_INTERNAL_168_TM=58.724 PRIMER_INTERNAL_168_GC_PERCENT=48.148 PRIMER_INTERNAL_168_SELF_ANY_TH=22.48 PRIMER_INTERNAL_168_SELF_END_TH=5.75 PRIMER_INTERNAL_168_HAIRPIN_TH=41.09 PRIMER_INTERNAL_169_PENALTY=8.300145 PRIMER_INTERNAL_169_SEQUENCE=ACACACACACACAGCACACAGGTATTA PRIMER_INTERNAL_169=37,27 PRIMER_INTERNAL_169_TM=58.700 PRIMER_INTERNAL_169_GC_PERCENT=44.444 PRIMER_INTERNAL_169_SELF_ANY_TH=0.00 PRIMER_INTERNAL_169_SELF_END_TH=0.00 PRIMER_INTERNAL_169_HAIRPIN_TH=0.00 PRIMER_INTERNAL_170_PENALTY=8.330872 PRIMER_INTERNAL_170_SEQUENCE=AGTAGACATGACACTGACGATGCAGA PRIMER_INTERNAL_170=7,26 PRIMER_INTERNAL_170_TM=57.669 PRIMER_INTERNAL_170_GC_PERCENT=46.154 PRIMER_INTERNAL_170_SELF_ANY_TH=7.76 PRIMER_INTERNAL_170_SELF_END_TH=0.00 PRIMER_INTERNAL_170_HAIRPIN_TH=0.00 PRIMER_INTERNAL_171_PENALTY=8.369366 PRIMER_INTERNAL_171_SEQUENCE=CACACAGCACACAGGTATTAGTGGGCC PRIMER_INTERNAL_171=44,27 PRIMER_INTERNAL_171_TM=61.369 PRIMER_INTERNAL_171_GC_PERCENT=55.556 PRIMER_INTERNAL_171_SELF_ANY_TH=8.63 PRIMER_INTERNAL_171_SELF_END_TH=0.00 PRIMER_INTERNAL_171_HAIRPIN_TH=32.68 PRIMER_INTERNAL_172_PENALTY=8.376203 PRIMER_INTERNAL_172_SEQUENCE=GTAGACATGACACTGACGATGCAGAC PRIMER_INTERNAL_172=8,26 PRIMER_INTERNAL_172_TM=57.624 PRIMER_INTERNAL_172_GC_PERCENT=50.000 PRIMER_INTERNAL_172_SELF_ANY_TH=1.44 PRIMER_INTERNAL_172_SELF_END_TH=0.00 PRIMER_INTERNAL_172_HAIRPIN_TH=0.00 PRIMER_INTERNAL_173_PENALTY=8.376203 PRIMER_INTERNAL_173_SEQUENCE=GTCAGTAGACATGACACTGACGATGC PRIMER_INTERNAL_173=4,26 PRIMER_INTERNAL_173_TM=57.624 PRIMER_INTERNAL_173_GC_PERCENT=50.000 PRIMER_INTERNAL_173_SELF_ANY_TH=22.48 PRIMER_INTERNAL_173_SELF_END_TH=10.90 PRIMER_INTERNAL_173_HAIRPIN_TH=41.09 PRIMER_INTERNAL_174_PENALTY=8.379879 PRIMER_INTERNAL_174_SEQUENCE=CAGTAGACATGACACTGACGATGCAG PRIMER_INTERNAL_174=6,26 PRIMER_INTERNAL_174_TM=57.620 PRIMER_INTERNAL_174_GC_PERCENT=50.000 PRIMER_INTERNAL_174_SELF_ANY_TH=12.05 PRIMER_INTERNAL_174_SELF_END_TH=0.00 PRIMER_INTERNAL_174_HAIRPIN_TH=0.00 PRIMER_INTERNAL_175_PENALTY=8.418488 PRIMER_INTERNAL_175_SEQUENCE=CACACACACACAGCACACAGGTATTA PRIMER_INTERNAL_175=38,26 PRIMER_INTERNAL_175_TM=57.582 PRIMER_INTERNAL_175_GC_PERCENT=46.154 PRIMER_INTERNAL_175_SELF_ANY_TH=0.00 PRIMER_INTERNAL_175_SELF_END_TH=0.00 PRIMER_INTERNAL_175_HAIRPIN_TH=0.00 PRIMER_INTERNAL_176_PENALTY=8.423914 PRIMER_INTERNAL_176_SEQUENCE=GACCCAAATCGATAGCTACGATGACG PRIMER_INTERNAL_176=81,26 PRIMER_INTERNAL_176_TM=57.576 PRIMER_INTERNAL_176_GC_PERCENT=50.000 PRIMER_INTERNAL_176_SELF_ANY_TH=1.59 PRIMER_INTERNAL_176_SELF_END_TH=0.00 PRIMER_INTERNAL_176_HAIRPIN_TH=43.02 PRIMER_INTERNAL_177_PENALTY=8.423914 PRIMER_INTERNAL_177_SEQUENCE=CGACCCAAATCGATAGCTACGATGAC PRIMER_INTERNAL_177=80,26 PRIMER_INTERNAL_177_TM=57.576 PRIMER_INTERNAL_177_GC_PERCENT=50.000 PRIMER_INTERNAL_177_SELF_ANY_TH=2.38 PRIMER_INTERNAL_177_SELF_END_TH=0.00 PRIMER_INTERNAL_177_HAIRPIN_TH=43.02 PRIMER_INTERNAL_178_PENALTY=8.452836 PRIMER_INTERNAL_178_SEQUENCE=CATTCGATCCCGACCCAAATCGATAG PRIMER_INTERNAL_178=70,26 PRIMER_INTERNAL_178_TM=57.547 PRIMER_INTERNAL_178_GC_PERCENT=50.000 PRIMER_INTERNAL_178_SELF_ANY_TH=0.00 PRIMER_INTERNAL_178_SELF_END_TH=0.00 PRIMER_INTERNAL_178_HAIRPIN_TH=41.60 PRIMER_INTERNAL_179_PENALTY=8.463323 PRIMER_INTERNAL_179_SEQUENCE=ACACAGGTATTAGTGGGCCATTCGATC PRIMER_INTERNAL_179=52,27 PRIMER_INTERNAL_179_TM=58.537 PRIMER_INTERNAL_179_GC_PERCENT=48.148 PRIMER_INTERNAL_179_SELF_ANY_TH=0.00 PRIMER_INTERNAL_179_SELF_END_TH=0.00 PRIMER_INTERNAL_179_HAIRPIN_TH=0.00 PRIMER_INTERNAL_180_PENALTY=8.486477 PRIMER_INTERNAL_180_SEQUENCE=CAGTAGACATGACACTGACGATGCAGA PRIMER_INTERNAL_180=6,27 PRIMER_INTERNAL_180_TM=58.514 PRIMER_INTERNAL_180_GC_PERCENT=48.148 PRIMER_INTERNAL_180_SELF_ANY_TH=12.05 PRIMER_INTERNAL_180_SELF_END_TH=0.00 PRIMER_INTERNAL_180_HAIRPIN_TH=0.00 PRIMER_INTERNAL_181_PENALTY=8.486477 PRIMER_INTERNAL_181_SEQUENCE=TCAGTAGACATGACACTGACGATGCAG PRIMER_INTERNAL_181=5,27 PRIMER_INTERNAL_181_TM=58.514 PRIMER_INTERNAL_181_GC_PERCENT=48.148 PRIMER_INTERNAL_181_SELF_ANY_TH=16.57 PRIMER_INTERNAL_181_SELF_END_TH=0.00 PRIMER_INTERNAL_181_HAIRPIN_TH=0.00 PRIMER_INTERNAL_182_PENALTY=8.487256 PRIMER_INTERNAL_182_SEQUENCE=AGTAGACATGACACTGACGATGCAGAC PRIMER_INTERNAL_182=7,27 PRIMER_INTERNAL_182_TM=58.513 PRIMER_INTERNAL_182_GC_PERCENT=48.148 PRIMER_INTERNAL_182_SELF_ANY_TH=1.44 PRIMER_INTERNAL_182_SELF_END_TH=0.00 PRIMER_INTERNAL_182_HAIRPIN_TH=0.00 PRIMER_INTERNAL_183_PENALTY=8.487256 PRIMER_INTERNAL_183_SEQUENCE=AGTCAGTAGACATGACACTGACGATGC PRIMER_INTERNAL_183=3,27 PRIMER_INTERNAL_183_TM=58.513 PRIMER_INTERNAL_183_GC_PERCENT=48.148 PRIMER_INTERNAL_183_SELF_ANY_TH=25.46 PRIMER_INTERNAL_183_SELF_END_TH=14.56 PRIMER_INTERNAL_183_HAIRPIN_TH=43.89 PRIMER_INTERNAL_184_PENALTY=8.514997 PRIMER_INTERNAL_184_SEQUENCE=ACACACACACAGCACACAGGTATTAGT PRIMER_INTERNAL_184=39,27 PRIMER_INTERNAL_184_TM=58.485 PRIMER_INTERNAL_184_GC_PERCENT=44.444 PRIMER_INTERNAL_184_SELF_ANY_TH=0.00 PRIMER_INTERNAL_184_SELF_END_TH=0.00 PRIMER_INTERNAL_184_HAIRPIN_TH=0.00 PRIMER_INTERNAL_185_PENALTY=8.547914 PRIMER_INTERNAL_185_SEQUENCE=TATTAGTGGGCCATTCGATCCCGACCC PRIMER_INTERNAL_185=59,27 PRIMER_INTERNAL_185_TM=61.548 PRIMER_INTERNAL_185_GC_PERCENT=55.556 PRIMER_INTERNAL_185_SELF_ANY_TH=5.61 PRIMER_INTERNAL_185_SELF_END_TH=2.93 PRIMER_INTERNAL_185_HAIRPIN_TH=41.60 PRIMER_INTERNAL_186_PENALTY=8.589885 PRIMER_INTERNAL_186_SEQUENCE=CACAGGTATTAGTGGGCCATTCGATC PRIMER_INTERNAL_186=53,26 PRIMER_INTERNAL_186_TM=57.410 PRIMER_INTERNAL_186_GC_PERCENT=50.000 PRIMER_INTERNAL_186_SELF_ANY_TH=0.00 PRIMER_INTERNAL_186_SELF_END_TH=0.00 PRIMER_INTERNAL_186_HAIRPIN_TH=0.00 PRIMER_INTERNAL_187_PENALTY=8.642220 PRIMER_INTERNAL_187_SEQUENCE=ACACACACAGCACACAGGTATTAGTG PRIMER_INTERNAL_187=41,26 PRIMER_INTERNAL_187_TM=57.358 PRIMER_INTERNAL_187_GC_PERCENT=46.154 PRIMER_INTERNAL_187_SELF_ANY_TH=0.00 PRIMER_INTERNAL_187_SELF_END_TH=0.00 PRIMER_INTERNAL_187_HAIRPIN_TH=0.00 PRIMER_INTERNAL_188_PENALTY=8.642220 PRIMER_INTERNAL_188_SEQUENCE=CACACACACAGCACACAGGTATTAGT PRIMER_INTERNAL_188=40,26 PRIMER_INTERNAL_188_TM=57.358 PRIMER_INTERNAL_188_GC_PERCENT=46.154 PRIMER_INTERNAL_188_SELF_ANY_TH=0.00 PRIMER_INTERNAL_188_SELF_END_TH=0.00 PRIMER_INTERNAL_188_HAIRPIN_TH=0.00 PRIMER_INTERNAL_189_PENALTY=8.642220 PRIMER_INTERNAL_189_SEQUENCE=ACACACACACAGCACACAGGTATTAG PRIMER_INTERNAL_189=39,26 PRIMER_INTERNAL_189_TM=57.358 PRIMER_INTERNAL_189_GC_PERCENT=46.154 PRIMER_INTERNAL_189_SELF_ANY_TH=0.00 PRIMER_INTERNAL_189_SELF_END_TH=0.00 PRIMER_INTERNAL_189_HAIRPIN_TH=0.00 PRIMER_INTERNAL_190_PENALTY=8.687221 PRIMER_INTERNAL_190_SEQUENCE=TGGGCCATTCGATCCCGACCCAAATC PRIMER_INTERNAL_190=65,26 PRIMER_INTERNAL_190_TM=62.687 PRIMER_INTERNAL_190_GC_PERCENT=57.692 PRIMER_INTERNAL_190_SELF_ANY_TH=8.58 PRIMER_INTERNAL_190_SELF_END_TH=0.00 PRIMER_INTERNAL_190_HAIRPIN_TH=41.29 PRIMER_INTERNAL_191_PENALTY=8.713667 PRIMER_INTERNAL_191_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGA PRIMER_INTERNAL_191=67,26 PRIMER_INTERNAL_191_TM=62.714 PRIMER_INTERNAL_191_GC_PERCENT=57.692 PRIMER_INTERNAL_191_SELF_ANY_TH=0.00 PRIMER_INTERNAL_191_SELF_END_TH=0.00 PRIMER_INTERNAL_191_HAIRPIN_TH=41.60 PRIMER_INTERNAL_192_PENALTY=8.714713 PRIMER_INTERNAL_192_SEQUENCE=ACACAGCACACAGGTATTAGTGGGCCA PRIMER_INTERNAL_192=45,27 PRIMER_INTERNAL_192_TM=61.715 PRIMER_INTERNAL_192_GC_PERCENT=51.852 PRIMER_INTERNAL_192_SELF_ANY_TH=8.63 PRIMER_INTERNAL_192_SELF_END_TH=1.50 PRIMER_INTERNAL_192_HAIRPIN_TH=32.68 PRIMER_INTERNAL_193_PENALTY=8.787348 PRIMER_INTERNAL_193_SEQUENCE=CACACACACAGCACACAGGTATTAGTG PRIMER_INTERNAL_193=40,27 PRIMER_INTERNAL_193_TM=58.213 PRIMER_INTERNAL_193_GC_PERCENT=48.148 PRIMER_INTERNAL_193_SELF_ANY_TH=0.00 PRIMER_INTERNAL_193_SELF_END_TH=0.00 PRIMER_INTERNAL_193_HAIRPIN_TH=0.00 PRIMER_INTERNAL_194_PENALTY=8.787348 PRIMER_INTERNAL_194_SEQUENCE=CACACACACACAGCACACAGGTATTAG PRIMER_INTERNAL_194=38,27 PRIMER_INTERNAL_194_TM=58.213 PRIMER_INTERNAL_194_GC_PERCENT=48.148 PRIMER_INTERNAL_194_SELF_ANY_TH=0.00 PRIMER_INTERNAL_194_SELF_END_TH=0.00 PRIMER_INTERNAL_194_HAIRPIN_TH=0.00 PRIMER_INTERNAL_195_PENALTY=8.904972 PRIMER_INTERNAL_195_SEQUENCE=GTGGGCCATTCGATCCCGACCCAAAT PRIMER_INTERNAL_195=64,26 PRIMER_INTERNAL_195_TM=62.905 PRIMER_INTERNAL_195_GC_PERCENT=57.692 PRIMER_INTERNAL_195_SELF_ANY_TH=8.58 PRIMER_INTERNAL_195_SELF_END_TH=0.00 PRIMER_INTERNAL_195_HAIRPIN_TH=43.79 PRIMER_INTERNAL_196_PENALTY=9.679371 PRIMER_INTERNAL_196_SEQUENCE=GGCCATTCGATCCCGACCCAAATCGAT PRIMER_INTERNAL_196=67,27 PRIMER_INTERNAL_196_TM=62.679 PRIMER_INTERNAL_196_GC_PERCENT=55.556 PRIMER_INTERNAL_196_SELF_ANY_TH=0.00 PRIMER_INTERNAL_196_SELF_END_TH=0.00 PRIMER_INTERNAL_196_HAIRPIN_TH=41.60 = PRIMER_PRODUCT_SIZE_RANGE=75-100 P3_FILE_FLAG=1 SEQUENCE_ID=pick_primer_list_and_file_flag_th_4 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_EXPLAIN=considered 349, too many Ns 5, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33 PRIMER_RIGHT_EXPLAIN=considered 347, too many Ns 3, GC content failed 1, low tm 275, high tm 13, high hairpin stability 6, long poly-x seq 16, ok 33 PRIMER_INTERNAL_EXPLAIN=considered 1078, too many Ns 12, GC content failed 5, low tm 941, high tm 8, high hairpin stability 17, long poly-x seq 22, ok 73 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=33 PRIMER_RIGHT_NUM_RETURNED=33 PRIMER_INTERNAL_NUM_RETURNED=73 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.486439 PRIMER_RIGHT_0_PENALTY=0.486439 PRIMER_INTERNAL_0_PENALTY=0.093814 PRIMER_LEFT_0_SEQUENCE=ATTCCAAAACGCAAAGCACG PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT PRIMER_INTERNAL_0_SEQUENCE=AACGCAAAGCACGCTCCCGA PRIMER_LEFT_0=118,20 PRIMER_RIGHT_0=137,20 PRIMER_INTERNAL_0=125,20 PRIMER_LEFT_0_TM=58.514 PRIMER_RIGHT_0_TM=58.514 PRIMER_INTERNAL_0_TM=59.906 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_SELF_ANY_TH=7.82 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=36.42 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon PRIMER_LEFT_0_END_STABILITY=5.3400 PRIMER_RIGHT_0_END_STABILITY=3.0100 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=8.2325 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0760 PRIMER_LEFT_1_PENALTY=1.160593 PRIMER_RIGHT_1_PENALTY=1.160593 PRIMER_INTERNAL_1_PENALTY=0.301069 PRIMER_LEFT_1_SEQUENCE=CAAAACGCAAAGCACGCTC PRIMER_RIGHT_1_SEQUENCE=GAGCGTGCTTTGCGTTTTG PRIMER_INTERNAL_1_SEQUENCE=ACGCAAAGCACGCTCCCGAT PRIMER_LEFT_1=122,19 PRIMER_RIGHT_1=140,19 PRIMER_INTERNAL_1=126,20 PRIMER_LEFT_1_TM=59.161 PRIMER_RIGHT_1_TM=59.161 PRIMER_INTERNAL_1_TM=59.699 PRIMER_LEFT_1_GC_PERCENT=52.632 PRIMER_RIGHT_1_GC_PERCENT=52.632 PRIMER_INTERNAL_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_SELF_ANY_TH=7.82 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=37.44 PRIMER_INTERNAL_1_HAIRPIN_TH=36.42 PRIMER_LEFT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_RIGHT_1_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_1_END_STABILITY=5.0300 PRIMER_RIGHT_1_END_STABILITY=2.4400 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=5.7937 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=8.3885 PRIMER_LEFT_2_PENALTY=1.656085 PRIMER_RIGHT_2_PENALTY=1.656085 PRIMER_INTERNAL_2_PENALTY=0.900305 PRIMER_LEFT_2_SEQUENCE=TATTCCAAAACGCAAAGCACG PRIMER_RIGHT_2_SEQUENCE=CGTGCTTTGCGTTTTGGAATA PRIMER_INTERNAL_2_SEQUENCE=AAACGCAAAGCACGCTCCCG PRIMER_LEFT_2=117,21 PRIMER_RIGHT_2=137,21 PRIMER_INTERNAL_2=124,20 PRIMER_LEFT_2_TM=58.344 PRIMER_RIGHT_2_TM=58.344 PRIMER_INTERNAL_2_TM=59.100 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_SELF_ANY_TH=1.50 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=1.50 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=41.14 PRIMER_LEFT_2_LIBRARY_MISPRIMING=10.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence PRIMER_RIGHT_2_LIBRARY_MISPRIMING=12.00, reverse MER46 Nonautonomous DNA transposon PRIMER_LEFT_2_END_STABILITY=5.3400 PRIMER_RIGHT_2_END_STABILITY=1.7500 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=10.3450 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=9.4271 PRIMER_LEFT_3_PENALTY=1.695359 PRIMER_RIGHT_3_PENALTY=1.695359 PRIMER_INTERNAL_3_PENALTY=0.956520 PRIMER_LEFT_3_SEQUENCE=TGACCTGATAGAAACGCCAAC PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA PRIMER_INTERNAL_3_SEQUENCE=CGCAAAGCACGCTCCCGATC PRIMER_LEFT_3=53,21 PRIMER_RIGHT_3=73,21 PRIMER_INTERNAL_3=127,20 PRIMER_LEFT_3_TM=58.305 PRIMER_RIGHT_3_TM=58.305 PRIMER_INTERNAL_3_TM=59.043 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=36.42 PRIMER_LEFT_3_LIBRARY_MISPRIMING=10.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene PRIMER_RIGHT_3_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=3.7700 PRIMER_RIGHT_3_END_STABILITY=4.0200 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=6.6359 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.3924 PRIMER_LEFT_4_PENALTY=1.831685 PRIMER_RIGHT_4_PENALTY=1.831685 PRIMER_INTERNAL_4_PENALTY=1.018091 PRIMER_LEFT_4_SEQUENCE=AACGCCAACTCACTGTATGA PRIMER_RIGHT_4_SEQUENCE=TCATACAGTGAGTTGGCGTT PRIMER_INTERNAL_4_SEQUENCE=AACGCAAAGCACGCTCCCGAT PRIMER_LEFT_4=65,20 PRIMER_RIGHT_4=84,20 PRIMER_INTERNAL_4=125,21 PRIMER_LEFT_4_TM=57.168 PRIMER_RIGHT_4_TM=57.168 PRIMER_INTERNAL_4_TM=59.982 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=57.143 PRIMER_INTERNAL_4_SELF_ANY_TH=7.82 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=35.45 PRIMER_RIGHT_4_HAIRPIN_TH=31.80 PRIMER_INTERNAL_4_HAIRPIN_TH=36.42 PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_RIGHT_4_LIBRARY_MISPRIMING=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_LEFT_4_END_STABILITY=2.1500 PRIMER_RIGHT_4_END_STABILITY=4.8400 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=18.7112 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=25.7856 PRIMER_LEFT_5_PENALTY=1.902705 PRIMER_RIGHT_5_PENALTY=1.902705 PRIMER_INTERNAL_5_PENALTY=1.178696 PRIMER_LEFT_5_SEQUENCE=AGAAACGCCAACTCACTGTA PRIMER_RIGHT_5_SEQUENCE=TACAGTGAGTTGGCGTTTCT PRIMER_INTERNAL_5_SEQUENCE=AAACGCAAAGCACGCTCCCGA PRIMER_LEFT_5=62,20 PRIMER_RIGHT_5=81,20 PRIMER_INTERNAL_5=124,21 PRIMER_LEFT_5_TM=57.097 PRIMER_RIGHT_5_TM=57.097 PRIMER_INTERNAL_5_TM=60.179 PRIMER_LEFT_5_GC_PERCENT=45.000 PRIMER_RIGHT_5_GC_PERCENT=45.000 PRIMER_INTERNAL_5_GC_PERCENT=57.143 PRIMER_INTERNAL_5_SELF_ANY_TH=7.82 PRIMER_LEFT_5_SELF_ANY_TH=0.00 PRIMER_RIGHT_5_SELF_ANY_TH=0.00 PRIMER_INTERNAL_5_SELF_END_TH=0.00 PRIMER_LEFT_5_SELF_END_TH=0.00 PRIMER_RIGHT_5_SELF_END_TH=0.00 PRIMER_LEFT_5_HAIRPIN_TH=0.00 PRIMER_RIGHT_5_HAIRPIN_TH=0.00 PRIMER_INTERNAL_5_HAIRPIN_TH=36.42 PRIMER_LEFT_5_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_5_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_5_END_STABILITY=2.7400 PRIMER_RIGHT_5_END_STABILITY=2.5200 PRIMER_LEFT_5_TEMPLATE_MISPRIMING_TH=15.5573 PRIMER_RIGHT_5_TEMPLATE_MISPRIMING_TH=19.2811 PRIMER_LEFT_6_PENALTY=1.902705 PRIMER_RIGHT_6_PENALTY=1.902705 PRIMER_INTERNAL_6_PENALTY=1.372876 PRIMER_LEFT_6_SEQUENCE=TAGAAACGCCAACTCACTGT PRIMER_RIGHT_6_SEQUENCE=ACAGTGAGTTGGCGTTTCTA PRIMER_INTERNAL_6_SEQUENCE=ACGCAAAGCACGCTCCCGATC PRIMER_LEFT_6=61,20 PRIMER_RIGHT_6=80,20 PRIMER_INTERNAL_6=126,21 PRIMER_LEFT_6_TM=57.097 PRIMER_RIGHT_6_TM=57.097 PRIMER_INTERNAL_6_TM=60.373 PRIMER_LEFT_6_GC_PERCENT=45.000 PRIMER_RIGHT_6_GC_PERCENT=45.000 PRIMER_INTERNAL_6_GC_PERCENT=61.905 PRIMER_INTERNAL_6_SELF_ANY_TH=7.82 PRIMER_LEFT_6_SELF_ANY_TH=0.00 PRIMER_RIGHT_6_SELF_ANY_TH=0.00 PRIMER_INTERNAL_6_SELF_END_TH=0.00 PRIMER_LEFT_6_SELF_END_TH=0.00 PRIMER_RIGHT_6_SELF_END_TH=0.00 PRIMER_LEFT_6_HAIRPIN_TH=0.00 PRIMER_RIGHT_6_HAIRPIN_TH=0.00 PRIMER_INTERNAL_6_HAIRPIN_TH=36.42 PRIMER_LEFT_6_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_6_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_6_END_STABILITY=3.5500 PRIMER_RIGHT_6_END_STABILITY=2.1000 PRIMER_LEFT_6_TEMPLATE_MISPRIMING_TH=10.6833 PRIMER_RIGHT_6_TEMPLATE_MISPRIMING_TH=20.2263 PRIMER_LEFT_7_PENALTY=1.950877 PRIMER_RIGHT_7_PENALTY=1.950877 PRIMER_INTERNAL_7_PENALTY=1.393730 PRIMER_LEFT_7_SEQUENCE=TTCCAAAACGCAAAGCACG PRIMER_RIGHT_7_SEQUENCE=CGTGCTTTGCGTTTTGGAA PRIMER_INTERNAL_7_SEQUENCE=ACGCAAAGCACGCTCCCGA PRIMER_LEFT_7=119,19 PRIMER_RIGHT_7=137,19 PRIMER_INTERNAL_7=126,19 PRIMER_LEFT_7_TM=58.049 PRIMER_RIGHT_7_TM=58.049 PRIMER_INTERNAL_7_TM=59.606 PRIMER_LEFT_7_GC_PERCENT=47.368 PRIMER_RIGHT_7_GC_PERCENT=47.368 PRIMER_INTERNAL_7_GC_PERCENT=63.158 PRIMER_INTERNAL_7_SELF_ANY_TH=7.82 PRIMER_LEFT_7_SELF_ANY_TH=0.00 PRIMER_RIGHT_7_SELF_ANY_TH=0.00 PRIMER_INTERNAL_7_SELF_END_TH=0.00 PRIMER_LEFT_7_SELF_END_TH=0.00 PRIMER_RIGHT_7_SELF_END_TH=0.00 PRIMER_LEFT_7_HAIRPIN_TH=0.00 PRIMER_RIGHT_7_HAIRPIN_TH=0.00 PRIMER_INTERNAL_7_HAIRPIN_TH=36.42 PRIMER_LEFT_7_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner PRIMER_RIGHT_7_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_7_END_STABILITY=5.3400 PRIMER_RIGHT_7_END_STABILITY=3.5300 PRIMER_LEFT_7_TEMPLATE_MISPRIMING_TH=2.1013 PRIMER_RIGHT_7_TEMPLATE_MISPRIMING_TH=4.8280 PRIMER_LEFT_8_PENALTY=1.972625 PRIMER_RIGHT_8_PENALTY=1.972625 PRIMER_INTERNAL_8_PENALTY=1.595076 PRIMER_LEFT_8_SEQUENCE=CCTGATAGAAACGCCAACTCA PRIMER_RIGHT_8_SEQUENCE=TGAGTTGGCGTTTCTATCAGG PRIMER_INTERNAL_8_SEQUENCE=AAAACGCAAAGCACGCTCCCG PRIMER_LEFT_8=56,21 PRIMER_RIGHT_8=76,21 PRIMER_INTERNAL_8=123,21 PRIMER_LEFT_8_TM=58.027 PRIMER_RIGHT_8_TM=58.027 PRIMER_INTERNAL_8_TM=59.405 PRIMER_LEFT_8_GC_PERCENT=47.619 PRIMER_RIGHT_8_GC_PERCENT=47.619 PRIMER_INTERNAL_8_GC_PERCENT=57.143 PRIMER_INTERNAL_8_SELF_ANY_TH=1.50 PRIMER_LEFT_8_SELF_ANY_TH=0.00 PRIMER_RIGHT_8_SELF_ANY_TH=0.00 PRIMER_INTERNAL_8_SELF_END_TH=1.50 PRIMER_LEFT_8_SELF_END_TH=0.00 PRIMER_RIGHT_8_SELF_END_TH=0.00 PRIMER_LEFT_8_HAIRPIN_TH=0.00 PRIMER_RIGHT_8_HAIRPIN_TH=0.00 PRIMER_INTERNAL_8_HAIRPIN_TH=41.14 PRIMER_LEFT_8_LIBRARY_MISPRIMING=10.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1) PRIMER_RIGHT_8_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene PRIMER_LEFT_8_END_STABILITY=3.4100 PRIMER_RIGHT_8_END_STABILITY=3.8600 PRIMER_LEFT_8_TEMPLATE_MISPRIMING_TH=20.8283 PRIMER_RIGHT_8_TEMPLATE_MISPRIMING_TH=12.8893 PRIMER_LEFT_9_PENALTY=1.973945 PRIMER_RIGHT_9_PENALTY=1.973945 PRIMER_INTERNAL_9_PENALTY=2.007622 PRIMER_LEFT_9_SEQUENCE=ACCTGATAGAAACGCCAACTC PRIMER_RIGHT_9_SEQUENCE=GAGTTGGCGTTTCTATCAGGT PRIMER_INTERNAL_9_SEQUENCE=CCAAAACGCAAAGCACGCTCCC PRIMER_LEFT_9=55,21 PRIMER_RIGHT_9=75,21 PRIMER_INTERNAL_9=121,22 PRIMER_LEFT_9_TM=58.026 PRIMER_RIGHT_9_TM=58.026 PRIMER_INTERNAL_9_TM=59.992 PRIMER_LEFT_9_GC_PERCENT=47.619 PRIMER_RIGHT_9_GC_PERCENT=47.619 PRIMER_INTERNAL_9_GC_PERCENT=59.091 PRIMER_INTERNAL_9_SELF_ANY_TH=0.00 PRIMER_LEFT_9_SELF_ANY_TH=0.00 PRIMER_RIGHT_9_SELF_ANY_TH=0.00 PRIMER_INTERNAL_9_SELF_END_TH=0.00 PRIMER_LEFT_9_SELF_END_TH=0.00 PRIMER_RIGHT_9_SELF_END_TH=0.00 PRIMER_LEFT_9_HAIRPIN_TH=0.00 PRIMER_RIGHT_9_HAIRPIN_TH=0.00 PRIMER_INTERNAL_9_HAIRPIN_TH=0.00 PRIMER_LEFT_9_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_9_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_9_END_STABILITY=3.0100 PRIMER_RIGHT_9_END_STABILITY=4.0000 PRIMER_LEFT_9_TEMPLATE_MISPRIMING_TH=6.8289 PRIMER_RIGHT_9_TEMPLATE_MISPRIMING_TH=11.0226 PRIMER_LEFT_10_PENALTY=1.973945 PRIMER_RIGHT_10_PENALTY=1.973945 PRIMER_INTERNAL_10_PENALTY=2.235780 PRIMER_LEFT_10_SEQUENCE=GACCTGATAGAAACGCCAACT PRIMER_RIGHT_10_SEQUENCE=AGTTGGCGTTTCTATCAGGTC PRIMER_INTERNAL_10_SEQUENCE=AACGCAAAGCACGCTCCCG PRIMER_LEFT_10=54,21 PRIMER_RIGHT_10=74,21 PRIMER_INTERNAL_10=125,19 PRIMER_LEFT_10_TM=58.026 PRIMER_RIGHT_10_TM=58.026 PRIMER_INTERNAL_10_TM=58.764 PRIMER_LEFT_10_GC_PERCENT=47.619 PRIMER_RIGHT_10_GC_PERCENT=47.619 PRIMER_INTERNAL_10_GC_PERCENT=63.158 PRIMER_INTERNAL_10_SELF_ANY_TH=1.50 PRIMER_LEFT_10_SELF_ANY_TH=0.00 PRIMER_RIGHT_10_SELF_ANY_TH=0.00 PRIMER_INTERNAL_10_SELF_END_TH=1.50 PRIMER_LEFT_10_SELF_END_TH=0.00 PRIMER_RIGHT_10_SELF_END_TH=0.00 PRIMER_LEFT_10_HAIRPIN_TH=0.00 PRIMER_RIGHT_10_HAIRPIN_TH=0.00 PRIMER_INTERNAL_10_HAIRPIN_TH=41.14 PRIMER_LEFT_10_LIBRARY_MISPRIMING=10.00, MER25 Older LINE1 5' end region - a consensus PRIMER_RIGHT_10_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_10_END_STABILITY=3.1600 PRIMER_RIGHT_10_END_STABILITY=3.8500 PRIMER_LEFT_10_TEMPLATE_MISPRIMING_TH=3.2290 PRIMER_RIGHT_10_TEMPLATE_MISPRIMING_TH=8.6567 PRIMER_LEFT_11_PENALTY=2.120321 PRIMER_RIGHT_11_PENALTY=2.120321 PRIMER_INTERNAL_11_PENALTY=2.240074 PRIMER_LEFT_11_SEQUENCE=AAACGCCAACTCACTGTATGA PRIMER_RIGHT_11_SEQUENCE=TCATACAGTGAGTTGGCGTTT PRIMER_INTERNAL_11_SEQUENCE=AAACGCAAAGCACGCTCCCGAT PRIMER_LEFT_11=64,21 PRIMER_RIGHT_11=84,21 PRIMER_INTERNAL_11=124,22 PRIMER_LEFT_11_TM=57.880 PRIMER_RIGHT_11_TM=57.880 PRIMER_INTERNAL_11_TM=60.240 PRIMER_LEFT_11_GC_PERCENT=42.857 PRIMER_RIGHT_11_GC_PERCENT=42.857 PRIMER_INTERNAL_11_GC_PERCENT=54.545 PRIMER_INTERNAL_11_SELF_ANY_TH=7.82 PRIMER_LEFT_11_SELF_ANY_TH=0.00 PRIMER_RIGHT_11_SELF_ANY_TH=0.00 PRIMER_INTERNAL_11_SELF_END_TH=0.00 PRIMER_LEFT_11_SELF_END_TH=0.00 PRIMER_RIGHT_11_SELF_END_TH=0.00 PRIMER_LEFT_11_HAIRPIN_TH=35.45 PRIMER_RIGHT_11_HAIRPIN_TH=31.80 PRIMER_INTERNAL_11_HAIRPIN_TH=36.42 PRIMER_LEFT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_RIGHT_11_LIBRARY_MISPRIMING=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence PRIMER_LEFT_11_END_STABILITY=2.1500 PRIMER_RIGHT_11_END_STABILITY=3.6000 PRIMER_LEFT_11_TEMPLATE_MISPRIMING_TH=5.5045 PRIMER_RIGHT_11_TEMPLATE_MISPRIMING_TH=24.8608 PRIMER_LEFT_12_PENALTY=2.142778 PRIMER_RIGHT_12_PENALTY=2.142778 PRIMER_INTERNAL_12_PENALTY=2.299276 PRIMER_LEFT_12_SEQUENCE=TTCCAAAACGCAAAGCACGC PRIMER_RIGHT_12_SEQUENCE=GCGTGCTTTGCGTTTTGGAA PRIMER_INTERNAL_12_SEQUENCE=CAAAACGCAAAGCACGCTCCCG PRIMER_LEFT_12=119,20 PRIMER_RIGHT_12=138,20 PRIMER_INTERNAL_12=122,22 PRIMER_LEFT_12_TM=61.143 PRIMER_RIGHT_12_TM=61.143 PRIMER_INTERNAL_12_TM=60.299 PRIMER_LEFT_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_GC_PERCENT=50.000 PRIMER_INTERNAL_12_GC_PERCENT=59.091 PRIMER_INTERNAL_12_SELF_ANY_TH=1.50 PRIMER_LEFT_12_SELF_ANY_TH=0.00 PRIMER_RIGHT_12_SELF_ANY_TH=0.00 PRIMER_INTERNAL_12_SELF_END_TH=1.50 PRIMER_LEFT_12_SELF_END_TH=0.00 PRIMER_RIGHT_12_SELF_END_TH=0.00 PRIMER_LEFT_12_HAIRPIN_TH=0.00 PRIMER_RIGHT_12_HAIRPIN_TH=0.00 PRIMER_INTERNAL_12_HAIRPIN_TH=41.14 PRIMER_LEFT_12_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner PRIMER_RIGHT_12_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_12_END_STABILITY=5.3400 PRIMER_RIGHT_12_END_STABILITY=3.5300 PRIMER_LEFT_12_TEMPLATE_MISPRIMING_TH=6.5778 PRIMER_RIGHT_12_TEMPLATE_MISPRIMING_TH=7.7746 PRIMER_LEFT_13_PENALTY=2.299089 PRIMER_RIGHT_13_PENALTY=2.299089 PRIMER_INTERNAL_13_PENALTY=2.427391 PRIMER_LEFT_13_SEQUENCE=AAAACGCAAAGCACGCTCC PRIMER_RIGHT_13_SEQUENCE=GGAGCGTGCTTTGCGTTTT PRIMER_INTERNAL_13_SEQUENCE=AAAACGCAAAGCACGCTCCCGA PRIMER_LEFT_13=123,19 PRIMER_RIGHT_13=141,19 PRIMER_INTERNAL_13=123,22 PRIMER_LEFT_13_TM=60.299 PRIMER_RIGHT_13_TM=60.299 PRIMER_INTERNAL_13_TM=60.427 PRIMER_LEFT_13_GC_PERCENT=52.632 PRIMER_RIGHT_13_GC_PERCENT=52.632 PRIMER_INTERNAL_13_GC_PERCENT=54.545 PRIMER_INTERNAL_13_SELF_ANY_TH=7.82 PRIMER_LEFT_13_SELF_ANY_TH=0.00 PRIMER_RIGHT_13_SELF_ANY_TH=0.00 PRIMER_INTERNAL_13_SELF_END_TH=0.00 PRIMER_LEFT_13_SELF_END_TH=0.00 PRIMER_RIGHT_13_SELF_END_TH=0.00 PRIMER_LEFT_13_HAIRPIN_TH=0.00 PRIMER_RIGHT_13_HAIRPIN_TH=37.44 PRIMER_INTERNAL_13_HAIRPIN_TH=36.42 PRIMER_LEFT_13_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_13_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_13_END_STABILITY=4.7000 PRIMER_RIGHT_13_END_STABILITY=2.4300 PRIMER_LEFT_13_TEMPLATE_MISPRIMING_TH=0.4266 PRIMER_RIGHT_13_TEMPLATE_MISPRIMING_TH=8.7659 PRIMER_LEFT_14_PENALTY=2.402628 PRIMER_RIGHT_14_PENALTY=2.402628 PRIMER_INTERNAL_14_PENALTY=2.610811 PRIMER_LEFT_14_SEQUENCE=AGAAACGCCAACTCACTGTAT PRIMER_RIGHT_14_SEQUENCE=ATACAGTGAGTTGGCGTTTCT PRIMER_INTERNAL_14_SEQUENCE=AACGCAAAGCACGCTCCCGATC PRIMER_LEFT_14=62,21 PRIMER_RIGHT_14=82,21 PRIMER_INTERNAL_14=125,22 PRIMER_LEFT_14_TM=57.597 PRIMER_RIGHT_14_TM=57.597 PRIMER_INTERNAL_14_TM=60.611 PRIMER_LEFT_14_GC_PERCENT=42.857 PRIMER_RIGHT_14_GC_PERCENT=42.857 PRIMER_INTERNAL_14_GC_PERCENT=59.091 PRIMER_INTERNAL_14_SELF_ANY_TH=7.82 PRIMER_LEFT_14_SELF_ANY_TH=0.00 PRIMER_RIGHT_14_SELF_ANY_TH=0.00 PRIMER_INTERNAL_14_SELF_END_TH=0.00 PRIMER_LEFT_14_SELF_END_TH=0.00 PRIMER_RIGHT_14_SELF_END_TH=0.00 PRIMER_LEFT_14_HAIRPIN_TH=0.00 PRIMER_RIGHT_14_HAIRPIN_TH=0.00 PRIMER_INTERNAL_14_HAIRPIN_TH=36.42 PRIMER_LEFT_14_LIBRARY_MISPRIMING=11.00, reverse MARINER1 Human mariner PRIMER_RIGHT_14_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_14_END_STABILITY=2.2900 PRIMER_RIGHT_14_END_STABILITY=2.5200 PRIMER_LEFT_14_TEMPLATE_MISPRIMING_TH=16.5430 PRIMER_RIGHT_14_TEMPLATE_MISPRIMING_TH=19.2811 PRIMER_LEFT_15_PENALTY=2.402628 PRIMER_RIGHT_15_PENALTY=2.402628 PRIMER_INTERNAL_15_PENALTY=2.740095 PRIMER_LEFT_15_SEQUENCE=ATAGAAACGCCAACTCACTGT PRIMER_RIGHT_15_SEQUENCE=ACAGTGAGTTGGCGTTTCTAT PRIMER_INTERNAL_15_SEQUENCE=CGCAAAGCACGCTCCCGAT PRIMER_LEFT_15=60,21 PRIMER_RIGHT_15=80,21 PRIMER_INTERNAL_15=127,19 PRIMER_LEFT_15_TM=57.597 PRIMER_RIGHT_15_TM=57.597 PRIMER_INTERNAL_15_TM=58.260 PRIMER_LEFT_15_GC_PERCENT=42.857 PRIMER_RIGHT_15_GC_PERCENT=42.857 PRIMER_INTERNAL_15_GC_PERCENT=63.158 PRIMER_INTERNAL_15_SELF_ANY_TH=0.00 PRIMER_LEFT_15_SELF_ANY_TH=0.00 PRIMER_RIGHT_15_SELF_ANY_TH=0.00 PRIMER_INTERNAL_15_SELF_END_TH=0.00 PRIMER_LEFT_15_SELF_END_TH=0.00 PRIMER_RIGHT_15_SELF_END_TH=0.00 PRIMER_LEFT_15_HAIRPIN_TH=0.00 PRIMER_RIGHT_15_HAIRPIN_TH=0.00 PRIMER_INTERNAL_15_HAIRPIN_TH=36.42 PRIMER_LEFT_15_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_15_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_15_END_STABILITY=3.5500 PRIMER_RIGHT_15_END_STABILITY=1.9800 PRIMER_LEFT_15_TEMPLATE_MISPRIMING_TH=14.5010 PRIMER_RIGHT_15_TEMPLATE_MISPRIMING_TH=21.0214 PRIMER_LEFT_16_PENALTY=2.455619 PRIMER_RIGHT_16_PENALTY=2.455619 PRIMER_INTERNAL_16_PENALTY=2.880381 PRIMER_LEFT_16_SEQUENCE=GAAACGCCAACTCACTGTATG PRIMER_RIGHT_16_SEQUENCE=CATACAGTGAGTTGGCGTTTC PRIMER_INTERNAL_16_SEQUENCE=TCCAAAACGCAAAGCACGCTCC PRIMER_LEFT_16=63,21 PRIMER_RIGHT_16=83,21 PRIMER_INTERNAL_16=120,22 PRIMER_LEFT_16_TM=57.544 PRIMER_RIGHT_16_TM=57.544 PRIMER_INTERNAL_16_TM=59.120 PRIMER_LEFT_16_GC_PERCENT=47.619 PRIMER_RIGHT_16_GC_PERCENT=47.619 PRIMER_INTERNAL_16_GC_PERCENT=54.545 PRIMER_INTERNAL_16_SELF_ANY_TH=0.00 PRIMER_LEFT_16_SELF_ANY_TH=0.00 PRIMER_RIGHT_16_SELF_ANY_TH=0.00 PRIMER_INTERNAL_16_SELF_END_TH=0.00 PRIMER_LEFT_16_SELF_END_TH=0.00 PRIMER_RIGHT_16_SELF_END_TH=0.00 PRIMER_LEFT_16_HAIRPIN_TH=0.00 PRIMER_RIGHT_16_HAIRPIN_TH=0.00 PRIMER_INTERNAL_16_HAIRPIN_TH=0.00 PRIMER_LEFT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner PRIMER_RIGHT_16_LIBRARY_MISPRIMING=12.00, reverse MARINER1 Human mariner PRIMER_LEFT_16_END_STABILITY=2.3900 PRIMER_RIGHT_16_END_STABILITY=2.7800 PRIMER_LEFT_16_TEMPLATE_MISPRIMING_TH=9.1798 PRIMER_RIGHT_16_TEMPLATE_MISPRIMING_TH=18.8273 PRIMER_LEFT_17_PENALTY=2.539776 PRIMER_RIGHT_17_PENALTY=2.539776 PRIMER_INTERNAL_17_PENALTY=2.939429 PRIMER_LEFT_17_SEQUENCE=CAAAACGCAAAGCACGCTCC PRIMER_RIGHT_17_SEQUENCE=GGAGCGTGCTTTGCGTTTTG PRIMER_INTERNAL_17_SEQUENCE=CAAAACGCAAAGCACGCTCCC PRIMER_LEFT_17=122,20 PRIMER_RIGHT_17=141,20 PRIMER_INTERNAL_17=122,21 PRIMER_LEFT_17_TM=61.540 PRIMER_RIGHT_17_TM=61.540 PRIMER_INTERNAL_17_TM=58.061 PRIMER_LEFT_17_GC_PERCENT=55.000 PRIMER_RIGHT_17_GC_PERCENT=55.000 PRIMER_INTERNAL_17_GC_PERCENT=57.143 PRIMER_INTERNAL_17_SELF_ANY_TH=0.00 PRIMER_LEFT_17_SELF_ANY_TH=0.00 PRIMER_RIGHT_17_SELF_ANY_TH=0.00 PRIMER_INTERNAL_17_SELF_END_TH=0.00 PRIMER_LEFT_17_SELF_END_TH=0.00 PRIMER_RIGHT_17_SELF_END_TH=0.00 PRIMER_LEFT_17_HAIRPIN_TH=0.00 PRIMER_RIGHT_17_HAIRPIN_TH=37.44 PRIMER_INTERNAL_17_HAIRPIN_TH=0.00 PRIMER_LEFT_17_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_17_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_17_END_STABILITY=4.7000 PRIMER_RIGHT_17_END_STABILITY=2.4400 PRIMER_LEFT_17_TEMPLATE_MISPRIMING_TH=4.7389 PRIMER_RIGHT_17_TEMPLATE_MISPRIMING_TH=6.1004 PRIMER_LEFT_18_PENALTY=2.539776 PRIMER_RIGHT_18_PENALTY=2.539776 PRIMER_INTERNAL_18_PENALTY=2.939429 PRIMER_LEFT_18_SEQUENCE=CCAAAACGCAAAGCACGCTC PRIMER_RIGHT_18_SEQUENCE=GAGCGTGCTTTGCGTTTTGG PRIMER_INTERNAL_18_SEQUENCE=CCAAAACGCAAAGCACGCTCC PRIMER_LEFT_18=121,20 PRIMER_RIGHT_18=140,20 PRIMER_INTERNAL_18=121,21 PRIMER_LEFT_18_TM=61.540 PRIMER_RIGHT_18_TM=61.540 PRIMER_INTERNAL_18_TM=58.061 PRIMER_LEFT_18_GC_PERCENT=55.000 PRIMER_RIGHT_18_GC_PERCENT=55.000 PRIMER_INTERNAL_18_GC_PERCENT=57.143 PRIMER_INTERNAL_18_SELF_ANY_TH=0.00 PRIMER_LEFT_18_SELF_ANY_TH=0.00 PRIMER_RIGHT_18_SELF_ANY_TH=0.00 PRIMER_INTERNAL_18_SELF_END_TH=0.00 PRIMER_LEFT_18_SELF_END_TH=0.00 PRIMER_RIGHT_18_SELF_END_TH=0.00 PRIMER_LEFT_18_HAIRPIN_TH=0.00 PRIMER_RIGHT_18_HAIRPIN_TH=37.44 PRIMER_INTERNAL_18_HAIRPIN_TH=0.00 PRIMER_LEFT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_RIGHT_18_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_18_END_STABILITY=5.0300 PRIMER_RIGHT_18_END_STABILITY=3.2800 PRIMER_LEFT_18_TEMPLATE_MISPRIMING_TH=7.7786 PRIMER_RIGHT_18_TEMPLATE_MISPRIMING_TH=9.7248 PRIMER_LEFT_19_PENALTY=2.587613 PRIMER_RIGHT_19_PENALTY=2.587613 PRIMER_INTERNAL_19_PENALTY=3.476553 PRIMER_LEFT_19_SEQUENCE=TCCAAAACGCAAAGCACGC PRIMER_RIGHT_19_SEQUENCE=GCGTGCTTTGCGTTTTGGA PRIMER_INTERNAL_19_SEQUENCE=AAAACGCAAAGCACGCTCCCGAT PRIMER_LEFT_19=120,19 PRIMER_RIGHT_19=138,19 PRIMER_INTERNAL_19=123,23 PRIMER_LEFT_19_TM=60.588 PRIMER_RIGHT_19_TM=60.588 PRIMER_INTERNAL_19_TM=60.477 PRIMER_LEFT_19_GC_PERCENT=52.632 PRIMER_RIGHT_19_GC_PERCENT=52.632 PRIMER_INTERNAL_19_GC_PERCENT=52.174 PRIMER_INTERNAL_19_SELF_ANY_TH=7.82 PRIMER_LEFT_19_SELF_ANY_TH=0.00 PRIMER_RIGHT_19_SELF_ANY_TH=0.00 PRIMER_INTERNAL_19_SELF_END_TH=0.00 PRIMER_LEFT_19_SELF_END_TH=0.00 PRIMER_RIGHT_19_SELF_END_TH=0.00 PRIMER_LEFT_19_HAIRPIN_TH=0.00 PRIMER_RIGHT_19_HAIRPIN_TH=0.00 PRIMER_INTERNAL_19_HAIRPIN_TH=36.42 PRIMER_LEFT_19_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner PRIMER_RIGHT_19_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_19_END_STABILITY=5.3400 PRIMER_RIGHT_19_END_STABILITY=3.5300 PRIMER_LEFT_19_TEMPLATE_MISPRIMING_TH=6.3533 PRIMER_RIGHT_19_TEMPLATE_MISPRIMING_TH=6.8599 PRIMER_LEFT_20_PENALTY=2.588667 PRIMER_RIGHT_20_PENALTY=2.588667 PRIMER_INTERNAL_20_PENALTY=3.598972 PRIMER_LEFT_20_SEQUENCE=CCAAAACGCAAAGCACGCT PRIMER_RIGHT_20_SEQUENCE=AGCGTGCTTTGCGTTTTGG PRIMER_INTERNAL_20_SEQUENCE=TTCCAAAACGCAAAGCACGCTCC PRIMER_LEFT_20=121,19 PRIMER_RIGHT_20=139,19 PRIMER_INTERNAL_20=119,23 PRIMER_LEFT_20_TM=60.589 PRIMER_RIGHT_20_TM=60.589 PRIMER_INTERNAL_20_TM=59.401 PRIMER_LEFT_20_GC_PERCENT=52.632 PRIMER_RIGHT_20_GC_PERCENT=52.632 PRIMER_INTERNAL_20_GC_PERCENT=52.174 PRIMER_INTERNAL_20_SELF_ANY_TH=0.00 PRIMER_LEFT_20_SELF_ANY_TH=0.00 PRIMER_RIGHT_20_SELF_ANY_TH=0.00 PRIMER_INTERNAL_20_SELF_END_TH=0.00 PRIMER_LEFT_20_SELF_END_TH=0.00 PRIMER_RIGHT_20_SELF_END_TH=0.00 PRIMER_LEFT_20_HAIRPIN_TH=0.00 PRIMER_RIGHT_20_HAIRPIN_TH=37.44 PRIMER_INTERNAL_20_HAIRPIN_TH=0.00 PRIMER_LEFT_20_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_20_LIBRARY_MISPRIMING=10.00, MARINER1 Human mariner PRIMER_LEFT_20_END_STABILITY=5.0700 PRIMER_RIGHT_20_END_STABILITY=3.2800 PRIMER_LEFT_20_TEMPLATE_MISPRIMING_TH=7.2070 PRIMER_RIGHT_20_TEMPLATE_MISPRIMING_TH=12.0561 PRIMER_LEFT_21_PENALTY=2.627395 PRIMER_RIGHT_21_PENALTY=2.627395 PRIMER_INTERNAL_21_PENALTY=3.606233 PRIMER_LEFT_21_SEQUENCE=ATGACCTGATAGAAACGCCAA PRIMER_RIGHT_21_SEQUENCE=TTGGCGTTTCTATCAGGTCAT PRIMER_INTERNAL_21_SEQUENCE=ACGCAAAGCACGCTCCCG PRIMER_LEFT_21=52,21 PRIMER_RIGHT_21=72,21 PRIMER_INTERNAL_21=126,18 PRIMER_LEFT_21_TM=57.373 PRIMER_RIGHT_21_TM=57.373 PRIMER_INTERNAL_21_TM=58.394 PRIMER_LEFT_21_GC_PERCENT=42.857 PRIMER_RIGHT_21_GC_PERCENT=42.857 PRIMER_INTERNAL_21_GC_PERCENT=66.667 PRIMER_INTERNAL_21_SELF_ANY_TH=1.50 PRIMER_LEFT_21_SELF_ANY_TH=0.00 PRIMER_RIGHT_21_SELF_ANY_TH=0.00 PRIMER_INTERNAL_21_SELF_END_TH=1.50 PRIMER_LEFT_21_SELF_END_TH=0.00 PRIMER_RIGHT_21_SELF_END_TH=0.00 PRIMER_LEFT_21_HAIRPIN_TH=0.00 PRIMER_RIGHT_21_HAIRPIN_TH=0.00 PRIMER_INTERNAL_21_HAIRPIN_TH=41.14 PRIMER_LEFT_21_LIBRARY_MISPRIMING=11.00, 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene PRIMER_RIGHT_21_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_21_END_STABILITY=4.5200 PRIMER_RIGHT_21_END_STABILITY=3.0600 PRIMER_LEFT_21_TEMPLATE_MISPRIMING_TH=3.5472 PRIMER_RIGHT_21_TEMPLATE_MISPRIMING_TH=0.2868 PRIMER_LEFT_22_PENALTY=2.678056 PRIMER_RIGHT_22_PENALTY=2.678056 PRIMER_INTERNAL_22_PENALTY=3.829005 PRIMER_LEFT_22_SEQUENCE=TGATAGAAACGCCAACTCACT PRIMER_RIGHT_22_SEQUENCE=AGTGAGTTGGCGTTTCTATCA PRIMER_INTERNAL_22_SEQUENCE=AAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_22=58,21 PRIMER_RIGHT_22=78,21 PRIMER_INTERNAL_22=124,23 PRIMER_LEFT_22_TM=57.322 PRIMER_RIGHT_22_TM=57.322 PRIMER_INTERNAL_22_TM=60.829 PRIMER_LEFT_22_GC_PERCENT=42.857 PRIMER_RIGHT_22_GC_PERCENT=42.857 PRIMER_INTERNAL_22_GC_PERCENT=56.522 PRIMER_INTERNAL_22_SELF_ANY_TH=7.82 PRIMER_LEFT_22_SELF_ANY_TH=0.00 PRIMER_RIGHT_22_SELF_ANY_TH=0.00 PRIMER_INTERNAL_22_SELF_END_TH=0.00 PRIMER_LEFT_22_SELF_END_TH=0.00 PRIMER_RIGHT_22_SELF_END_TH=0.00 PRIMER_LEFT_22_HAIRPIN_TH=0.00 PRIMER_RIGHT_22_HAIRPIN_TH=0.00 PRIMER_INTERNAL_22_HAIRPIN_TH=36.42 PRIMER_LEFT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_22_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_22_END_STABILITY=3.4100 PRIMER_RIGHT_22_END_STABILITY=2.1500 PRIMER_LEFT_22_TEMPLATE_MISPRIMING_TH=16.4194 PRIMER_RIGHT_22_TEMPLATE_MISPRIMING_TH=13.4901 PRIMER_LEFT_23_PENALTY=2.780624 PRIMER_RIGHT_23_PENALTY=2.780624 PRIMER_INTERNAL_23_PENALTY=3.865064 PRIMER_LEFT_23_SEQUENCE=AGAAACGCCAACTCACTGT PRIMER_RIGHT_23_SEQUENCE=ACAGTGAGTTGGCGTTTCT PRIMER_INTERNAL_23_SEQUENCE=TCCAAAACGCAAAGCACGCTC PRIMER_LEFT_23=62,19 PRIMER_RIGHT_23=80,19 PRIMER_INTERNAL_23=120,21 PRIMER_LEFT_23_TM=57.219 PRIMER_RIGHT_23_TM=57.219 PRIMER_INTERNAL_23_TM=57.135 PRIMER_LEFT_23_GC_PERCENT=47.368 PRIMER_RIGHT_23_GC_PERCENT=47.368 PRIMER_INTERNAL_23_GC_PERCENT=52.381 PRIMER_INTERNAL_23_SELF_ANY_TH=0.00 PRIMER_LEFT_23_SELF_ANY_TH=0.00 PRIMER_RIGHT_23_SELF_ANY_TH=0.00 PRIMER_INTERNAL_23_SELF_END_TH=0.00 PRIMER_LEFT_23_SELF_END_TH=0.00 PRIMER_RIGHT_23_SELF_END_TH=0.00 PRIMER_LEFT_23_HAIRPIN_TH=0.00 PRIMER_RIGHT_23_HAIRPIN_TH=0.00 PRIMER_INTERNAL_23_HAIRPIN_TH=0.00 PRIMER_LEFT_23_LIBRARY_MISPRIMING=11.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_23_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_23_END_STABILITY=3.5500 PRIMER_RIGHT_23_END_STABILITY=2.5200 PRIMER_LEFT_23_TEMPLATE_MISPRIMING_TH=8.5732 PRIMER_RIGHT_23_TEMPLATE_MISPRIMING_TH=19.2811 PRIMER_LEFT_24_PENALTY=2.999071 PRIMER_RIGHT_24_PENALTY=2.999071 PRIMER_INTERNAL_24_PENALTY=3.905453 PRIMER_LEFT_24_SEQUENCE=TCCAAAACGCAAAGCACGCT PRIMER_RIGHT_24_SEQUENCE=AGCGTGCTTTGCGTTTTGGA PRIMER_INTERNAL_24_SEQUENCE=CGCAAAGCACGCTCCCGA PRIMER_LEFT_24=120,20 PRIMER_RIGHT_24=139,20 PRIMER_INTERNAL_24=127,18 PRIMER_LEFT_24_TM=61.999 PRIMER_RIGHT_24_TM=61.999 PRIMER_INTERNAL_24_TM=58.095 PRIMER_LEFT_24_GC_PERCENT=50.000 PRIMER_RIGHT_24_GC_PERCENT=50.000 PRIMER_INTERNAL_24_GC_PERCENT=66.667 PRIMER_INTERNAL_24_SELF_ANY_TH=0.00 PRIMER_LEFT_24_SELF_ANY_TH=0.00 PRIMER_RIGHT_24_SELF_ANY_TH=0.00 PRIMER_INTERNAL_24_SELF_END_TH=0.00 PRIMER_LEFT_24_SELF_END_TH=0.00 PRIMER_RIGHT_24_SELF_END_TH=0.00 PRIMER_LEFT_24_HAIRPIN_TH=0.00 PRIMER_RIGHT_24_HAIRPIN_TH=37.44 PRIMER_INTERNAL_24_HAIRPIN_TH=36.42 PRIMER_LEFT_24_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_24_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_24_END_STABILITY=5.0700 PRIMER_RIGHT_24_END_STABILITY=3.5300 PRIMER_LEFT_24_TEMPLATE_MISPRIMING_TH=8.9249 PRIMER_RIGHT_24_TEMPLATE_MISPRIMING_TH=6.8599 PRIMER_LEFT_25_PENALTY=2.999229 PRIMER_RIGHT_25_PENALTY=2.999229 PRIMER_INTERNAL_25_PENALTY=3.954555 PRIMER_LEFT_25_SEQUENCE=GATAGAAACGCCAACTCACTG PRIMER_RIGHT_25_SEQUENCE=CAGTGAGTTGGCGTTTCTATC PRIMER_INTERNAL_25_SEQUENCE=TCCAAAACGCAAAGCACGCTCCC PRIMER_LEFT_25=59,21 PRIMER_RIGHT_25=79,21 PRIMER_INTERNAL_25=120,23 PRIMER_LEFT_25_TM=57.001 PRIMER_RIGHT_25_TM=57.001 PRIMER_INTERNAL_25_TM=60.955 PRIMER_LEFT_25_GC_PERCENT=47.619 PRIMER_RIGHT_25_GC_PERCENT=47.619 PRIMER_INTERNAL_25_GC_PERCENT=56.522 PRIMER_INTERNAL_25_SELF_ANY_TH=0.00 PRIMER_LEFT_25_SELF_ANY_TH=0.00 PRIMER_RIGHT_25_SELF_ANY_TH=0.00 PRIMER_INTERNAL_25_SELF_END_TH=0.00 PRIMER_LEFT_25_SELF_END_TH=0.00 PRIMER_RIGHT_25_SELF_END_TH=0.00 PRIMER_LEFT_25_HAIRPIN_TH=0.00 PRIMER_RIGHT_25_HAIRPIN_TH=0.00 PRIMER_INTERNAL_25_HAIRPIN_TH=0.00 PRIMER_LEFT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_RIGHT_25_LIBRARY_MISPRIMING=12.00, LOR1 Human low copy repetitive sequence - a consensus PRIMER_LEFT_25_END_STABILITY=3.6600 PRIMER_RIGHT_25_END_STABILITY=2.0800 PRIMER_LEFT_25_TEMPLATE_MISPRIMING_TH=19.5396 PRIMER_RIGHT_25_TEMPLATE_MISPRIMING_TH=18.4032 PRIMER_LEFT_26_PENALTY=2.999229 PRIMER_RIGHT_26_PENALTY=2.999229 PRIMER_INTERNAL_26_PENALTY=4.240075 PRIMER_LEFT_26_SEQUENCE=CTGATAGAAACGCCAACTCAC PRIMER_RIGHT_26_SEQUENCE=GTGAGTTGGCGTTTCTATCAG PRIMER_INTERNAL_26_SEQUENCE=CAAAACGCAAAGCACGCTCCCGA PRIMER_LEFT_26=57,21 PRIMER_RIGHT_26=77,21 PRIMER_INTERNAL_26=122,23 PRIMER_LEFT_26_TM=57.001 PRIMER_RIGHT_26_TM=57.001 PRIMER_INTERNAL_26_TM=61.240 PRIMER_LEFT_26_GC_PERCENT=47.619 PRIMER_RIGHT_26_GC_PERCENT=47.619 PRIMER_INTERNAL_26_GC_PERCENT=56.522 PRIMER_INTERNAL_26_SELF_ANY_TH=7.82 PRIMER_LEFT_26_SELF_ANY_TH=0.00 PRIMER_RIGHT_26_SELF_ANY_TH=0.00 PRIMER_INTERNAL_26_SELF_END_TH=0.00 PRIMER_LEFT_26_SELF_END_TH=0.00 PRIMER_RIGHT_26_SELF_END_TH=0.00 PRIMER_LEFT_26_HAIRPIN_TH=0.00 PRIMER_RIGHT_26_HAIRPIN_TH=0.00 PRIMER_INTERNAL_26_HAIRPIN_TH=36.42 PRIMER_LEFT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_26_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_26_END_STABILITY=3.5100 PRIMER_RIGHT_26_END_STABILITY=2.9000 PRIMER_LEFT_26_TEMPLATE_MISPRIMING_TH=13.7640 PRIMER_RIGHT_26_TEMPLATE_MISPRIMING_TH=15.9212 PRIMER_LEFT_27_PENALTY=3.455227 PRIMER_RIGHT_27_PENALTY=3.455227 PRIMER_INTERNAL_27_PENALTY=4.486709 PRIMER_LEFT_27_SEQUENCE=ATTCCAAAACGCAAAGCACGC PRIMER_RIGHT_27_SEQUENCE=GCGTGCTTTGCGTTTTGGAAT PRIMER_INTERNAL_27_SEQUENCE=TTCCAAAACGCAAAGCACGCTC PRIMER_LEFT_27=118,21 PRIMER_RIGHT_27=138,21 PRIMER_INTERNAL_27=119,22 PRIMER_LEFT_27_TM=61.455 PRIMER_RIGHT_27_TM=61.455 PRIMER_INTERNAL_27_TM=57.513 PRIMER_LEFT_27_GC_PERCENT=47.619 PRIMER_RIGHT_27_GC_PERCENT=47.619 PRIMER_INTERNAL_27_GC_PERCENT=50.000 PRIMER_INTERNAL_27_SELF_ANY_TH=0.00 PRIMER_LEFT_27_SELF_ANY_TH=0.00 PRIMER_RIGHT_27_SELF_ANY_TH=0.00 PRIMER_INTERNAL_27_SELF_END_TH=0.00 PRIMER_LEFT_27_SELF_END_TH=0.00 PRIMER_RIGHT_27_SELF_END_TH=0.00 PRIMER_LEFT_27_HAIRPIN_TH=0.00 PRIMER_RIGHT_27_HAIRPIN_TH=0.00 PRIMER_INTERNAL_27_HAIRPIN_TH=0.00 PRIMER_LEFT_27_LIBRARY_MISPRIMING=9.00, MARINER1 Human mariner PRIMER_RIGHT_27_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon PRIMER_LEFT_27_END_STABILITY=5.3400 PRIMER_RIGHT_27_END_STABILITY=3.0100 PRIMER_LEFT_27_TEMPLATE_MISPRIMING_TH=1.8923 PRIMER_RIGHT_27_TEMPLATE_MISPRIMING_TH=11.3682 PRIMER_LEFT_28_PENALTY=3.677573 PRIMER_RIGHT_28_PENALTY=3.677573 PRIMER_INTERNAL_28_PENALTY=4.513970 PRIMER_LEFT_28_SEQUENCE=AAAACGCAAAGCACGCTCCC PRIMER_RIGHT_28_SEQUENCE=GGGAGCGTGCTTTGCGTTTT PRIMER_INTERNAL_28_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCC PRIMER_LEFT_28=123,20 PRIMER_RIGHT_28=142,20 PRIMER_INTERNAL_28=118,24 PRIMER_LEFT_28_TM=62.678 PRIMER_RIGHT_28_TM=62.678 PRIMER_INTERNAL_28_TM=59.486 PRIMER_LEFT_28_GC_PERCENT=55.000 PRIMER_RIGHT_28_GC_PERCENT=55.000 PRIMER_INTERNAL_28_GC_PERCENT=50.000 PRIMER_INTERNAL_28_SELF_ANY_TH=0.00 PRIMER_LEFT_28_SELF_ANY_TH=0.00 PRIMER_RIGHT_28_SELF_ANY_TH=0.00 PRIMER_INTERNAL_28_SELF_END_TH=0.00 PRIMER_LEFT_28_SELF_END_TH=0.00 PRIMER_RIGHT_28_SELF_END_TH=0.00 PRIMER_LEFT_28_HAIRPIN_TH=0.00 PRIMER_RIGHT_28_HAIRPIN_TH=37.44 PRIMER_INTERNAL_28_HAIRPIN_TH=0.00 PRIMER_LEFT_28_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1) PRIMER_RIGHT_28_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_28_END_STABILITY=4.3000 PRIMER_RIGHT_28_END_STABILITY=2.4300 PRIMER_LEFT_28_TEMPLATE_MISPRIMING_TH=0.0612 PRIMER_RIGHT_28_TEMPLATE_MISPRIMING_TH=1.5624 PRIMER_LEFT_29_PENALTY=4.100862 PRIMER_RIGHT_29_PENALTY=4.100862 PRIMER_INTERNAL_29_PENALTY=5.029817 PRIMER_LEFT_29_SEQUENCE=GCAAAGCACGCTCCCGATC PRIMER_RIGHT_29_SEQUENCE=GATCGGGAGCGTGCTTTGC PRIMER_INTERNAL_29_SEQUENCE=AAAACGCAAAGCACGCTCCCGATC PRIMER_LEFT_29=128,19 PRIMER_RIGHT_29=146,19 PRIMER_INTERNAL_29=123,24 PRIMER_LEFT_29_TM=62.101 PRIMER_RIGHT_29_TM=62.101 PRIMER_INTERNAL_29_TM=61.030 PRIMER_LEFT_29_GC_PERCENT=63.158 PRIMER_RIGHT_29_GC_PERCENT=63.158 PRIMER_INTERNAL_29_GC_PERCENT=54.167 PRIMER_INTERNAL_29_SELF_ANY_TH=0.00 PRIMER_LEFT_29_SELF_ANY_TH=0.00 PRIMER_RIGHT_29_SELF_ANY_TH=0.00 PRIMER_INTERNAL_29_SELF_END_TH=0.00 PRIMER_LEFT_29_SELF_END_TH=0.00 PRIMER_RIGHT_29_SELF_END_TH=0.00 PRIMER_LEFT_29_HAIRPIN_TH=38.29 PRIMER_RIGHT_29_HAIRPIN_TH=0.00 PRIMER_INTERNAL_29_HAIRPIN_TH=36.42 PRIMER_LEFT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon PRIMER_RIGHT_29_LIBRARY_MISPRIMING=10.00, MER1A Nonautonomous DNA transposon PRIMER_LEFT_29_END_STABILITY=3.6900 PRIMER_RIGHT_29_END_STABILITY=3.6800 PRIMER_LEFT_29_TEMPLATE_MISPRIMING_TH=5.4030 PRIMER_RIGHT_29_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_LEFT_30_PENALTY=4.194272 PRIMER_RIGHT_30_PENALTY=4.194272 PRIMER_INTERNAL_30_PENALTY=5.052238 PRIMER_LEFT_30_SEQUENCE=AAACGCAAAGCACGCTCCC PRIMER_RIGHT_30_SEQUENCE=GGGAGCGTGCTTTGCGTTT PRIMER_INTERNAL_30_SEQUENCE=CCAAAACGCAAAGCACGCTCCCG PRIMER_LEFT_30=124,19 PRIMER_RIGHT_30=142,19 PRIMER_INTERNAL_30=121,23 PRIMER_LEFT_30_TM=62.194 PRIMER_RIGHT_30_TM=62.194 PRIMER_INTERNAL_30_TM=62.052 PRIMER_LEFT_30_GC_PERCENT=57.895 PRIMER_RIGHT_30_GC_PERCENT=57.895 PRIMER_INTERNAL_30_GC_PERCENT=60.870 PRIMER_INTERNAL_30_SELF_ANY_TH=1.50 PRIMER_LEFT_30_SELF_ANY_TH=0.00 PRIMER_RIGHT_30_SELF_ANY_TH=0.00 PRIMER_INTERNAL_30_SELF_END_TH=1.50 PRIMER_LEFT_30_SELF_END_TH=0.00 PRIMER_RIGHT_30_SELF_END_TH=0.00 PRIMER_LEFT_30_HAIRPIN_TH=0.00 PRIMER_RIGHT_30_HAIRPIN_TH=37.44 PRIMER_INTERNAL_30_HAIRPIN_TH=41.14 PRIMER_LEFT_30_LIBRARY_MISPRIMING=9.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1) PRIMER_RIGHT_30_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_LEFT_30_END_STABILITY=4.3000 PRIMER_RIGHT_30_END_STABILITY=3.6000 PRIMER_LEFT_30_TEMPLATE_MISPRIMING_TH=5.4315 PRIMER_RIGHT_30_TEMPLATE_MISPRIMING_TH=7.1743 PRIMER_LEFT_31_PENALTY=4.473143 PRIMER_RIGHT_31_PENALTY=4.473143 PRIMER_INTERNAL_31_PENALTY=5.152054 PRIMER_LEFT_31_SEQUENCE=TTCCAAAACGCAAAGCACGCT PRIMER_RIGHT_31_SEQUENCE=AGCGTGCTTTGCGTTTTGGAA PRIMER_INTERNAL_31_SEQUENCE=TTCCAAAACGCAAAGCACGCTCCC PRIMER_LEFT_31=119,21 PRIMER_RIGHT_31=139,21 PRIMER_INTERNAL_31=119,24 PRIMER_LEFT_31_TM=62.473 PRIMER_RIGHT_31_TM=62.473 PRIMER_INTERNAL_31_TM=61.152 PRIMER_LEFT_31_GC_PERCENT=47.619 PRIMER_RIGHT_31_GC_PERCENT=47.619 PRIMER_INTERNAL_31_GC_PERCENT=54.167 PRIMER_INTERNAL_31_SELF_ANY_TH=0.00 PRIMER_LEFT_31_SELF_ANY_TH=0.00 PRIMER_RIGHT_31_SELF_ANY_TH=0.00 PRIMER_INTERNAL_31_SELF_END_TH=0.00 PRIMER_LEFT_31_SELF_END_TH=0.00 PRIMER_RIGHT_31_SELF_END_TH=0.00 PRIMER_LEFT_31_HAIRPIN_TH=0.00 PRIMER_RIGHT_31_HAIRPIN_TH=37.44 PRIMER_INTERNAL_31_HAIRPIN_TH=0.00 PRIMER_LEFT_31_LIBRARY_MISPRIMING=9.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3) PRIMER_RIGHT_31_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_31_END_STABILITY=5.0700 PRIMER_RIGHT_31_END_STABILITY=3.5300 PRIMER_LEFT_31_TEMPLATE_MISPRIMING_TH=0.0000 PRIMER_RIGHT_31_TEMPLATE_MISPRIMING_TH=9.3017 PRIMER_LEFT_32_PENALTY=4.837196 PRIMER_RIGHT_32_PENALTY=4.837196 PRIMER_INTERNAL_32_PENALTY=5.256814 PRIMER_LEFT_32_SEQUENCE=TCCAAAACGCAAAGCACGCTC PRIMER_RIGHT_32_SEQUENCE=GAGCGTGCTTTGCGTTTTGGA PRIMER_INTERNAL_32_SEQUENCE=CAAAACGCAAAGCACGCTCCCGAT PRIMER_LEFT_32=120,21 PRIMER_RIGHT_32=140,21 PRIMER_INTERNAL_32=122,24 PRIMER_LEFT_32_TM=62.837 PRIMER_RIGHT_32_TM=62.837 PRIMER_INTERNAL_32_TM=61.257 PRIMER_LEFT_32_GC_PERCENT=52.381 PRIMER_RIGHT_32_GC_PERCENT=52.381 PRIMER_INTERNAL_32_GC_PERCENT=54.167 PRIMER_INTERNAL_32_SELF_ANY_TH=7.82 PRIMER_LEFT_32_SELF_ANY_TH=0.00 PRIMER_RIGHT_32_SELF_ANY_TH=0.00 PRIMER_INTERNAL_32_SELF_END_TH=0.00 PRIMER_LEFT_32_SELF_END_TH=0.00 PRIMER_RIGHT_32_SELF_END_TH=0.00 PRIMER_LEFT_32_HAIRPIN_TH=0.00 PRIMER_RIGHT_32_HAIRPIN_TH=37.44 PRIMER_INTERNAL_32_HAIRPIN_TH=36.42 PRIMER_LEFT_32_LIBRARY_MISPRIMING=10.00, TAR1 Human telomere associated repeat sequence, complete sequence PRIMER_RIGHT_32_LIBRARY_MISPRIMING=10.00, MER46 Nonautonomous DNA transposon PRIMER_LEFT_32_END_STABILITY=5.0300 PRIMER_RIGHT_32_END_STABILITY=3.5300 PRIMER_LEFT_32_TEMPLATE_MISPRIMING_TH=8.9959 PRIMER_RIGHT_32_TEMPLATE_MISPRIMING_TH=4.7683 PRIMER_INTERNAL_33_PENALTY=5.325970 PRIMER_INTERNAL_33_SEQUENCE=ATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_33=118,23 PRIMER_INTERNAL_33_TM=57.674 PRIMER_INTERNAL_33_GC_PERCENT=47.826 PRIMER_INTERNAL_33_SELF_ANY_TH=0.00 PRIMER_INTERNAL_33_SELF_END_TH=0.00 PRIMER_INTERNAL_33_HAIRPIN_TH=0.00 PRIMER_INTERNAL_34_PENALTY=5.931698 PRIMER_INTERNAL_34_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCC PRIMER_INTERNAL_34=117,25 PRIMER_INTERNAL_34_TM=59.068 PRIMER_INTERNAL_34_GC_PERCENT=48.000 PRIMER_INTERNAL_34_SELF_ANY_TH=0.00 PRIMER_INTERNAL_34_SELF_END_TH=0.00 PRIMER_INTERNAL_34_HAIRPIN_TH=0.00 PRIMER_INTERNAL_35_PENALTY=6.171494 PRIMER_INTERNAL_35_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCC PRIMER_INTERNAL_35=118,25 PRIMER_INTERNAL_35_TM=61.171 PRIMER_INTERNAL_35_GC_PERCENT=52.000 PRIMER_INTERNAL_35_SELF_ANY_TH=0.00 PRIMER_INTERNAL_35_SELF_END_TH=0.00 PRIMER_INTERNAL_35_HAIRPIN_TH=0.00 PRIMER_INTERNAL_36_PENALTY=6.354311 PRIMER_INTERNAL_36_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCC PRIMER_INTERNAL_36=116,26 PRIMER_INTERNAL_36_TM=59.646 PRIMER_INTERNAL_36_GC_PERCENT=50.000 PRIMER_INTERNAL_36_SELF_ANY_TH=0.00 PRIMER_INTERNAL_36_SELF_END_TH=0.00 PRIMER_INTERNAL_36_HAIRPIN_TH=0.00 PRIMER_INTERNAL_37_PENALTY=6.689016 PRIMER_INTERNAL_37_SEQUENCE=TATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_37=117,24 PRIMER_INTERNAL_37_TM=57.311 PRIMER_INTERNAL_37_GC_PERCENT=45.833 PRIMER_INTERNAL_37_SELF_ANY_TH=0.00 PRIMER_INTERNAL_37_SELF_END_TH=0.00 PRIMER_INTERNAL_37_HAIRPIN_TH=0.00 PRIMER_INTERNAL_38_PENALTY=6.689016 PRIMER_INTERNAL_38_SEQUENCE=TCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_38=115,24 PRIMER_INTERNAL_38_TM=57.311 PRIMER_INTERNAL_38_GC_PERCENT=45.833 PRIMER_INTERNAL_38_SELF_ANY_TH=0.00 PRIMER_INTERNAL_38_SELF_END_TH=0.00 PRIMER_INTERNAL_38_HAIRPIN_TH=0.00 PRIMER_INTERNAL_39_PENALTY=6.694952 PRIMER_INTERNAL_39_SEQUENCE=CTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_39=116,24 PRIMER_INTERNAL_39_TM=57.305 PRIMER_INTERNAL_39_GC_PERCENT=45.833 PRIMER_INTERNAL_39_SELF_ANY_TH=0.00 PRIMER_INTERNAL_39_SELF_END_TH=0.00 PRIMER_INTERNAL_39_HAIRPIN_TH=0.00 PRIMER_INTERNAL_40_PENALTY=6.707310 PRIMER_INTERNAL_40_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCC PRIMER_INTERNAL_40=117,26 PRIMER_INTERNAL_40_TM=60.707 PRIMER_INTERNAL_40_GC_PERCENT=50.000 PRIMER_INTERNAL_40_SELF_ANY_TH=0.00 PRIMER_INTERNAL_40_SELF_END_TH=0.00 PRIMER_INTERNAL_40_HAIRPIN_TH=0.00 PRIMER_INTERNAL_41_PENALTY=6.724062 PRIMER_INTERNAL_41_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_41=115,25 PRIMER_INTERNAL_41_TM=58.276 PRIMER_INTERNAL_41_GC_PERCENT=44.000 PRIMER_INTERNAL_41_SELF_ANY_TH=0.00 PRIMER_INTERNAL_41_SELF_END_TH=0.00 PRIMER_INTERNAL_41_HAIRPIN_TH=0.00 PRIMER_INTERNAL_42_PENALTY=6.744217 PRIMER_INTERNAL_42_SEQUENCE=CAAAACGCAAAGCACGCTCCCGATC PRIMER_INTERNAL_42=122,25 PRIMER_INTERNAL_42_TM=61.744 PRIMER_INTERNAL_42_GC_PERCENT=56.000 PRIMER_INTERNAL_42_SELF_ANY_TH=0.00 PRIMER_INTERNAL_42_SELF_END_TH=0.00 PRIMER_INTERNAL_42_HAIRPIN_TH=36.42 PRIMER_INTERNAL_43_PENALTY=6.785590 PRIMER_INTERNAL_43_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_43=114,25 PRIMER_INTERNAL_43_TM=58.214 PRIMER_INTERNAL_43_GC_PERCENT=48.000 PRIMER_INTERNAL_43_SELF_ANY_TH=0.00 PRIMER_INTERNAL_43_SELF_END_TH=0.00 PRIMER_INTERNAL_43_HAIRPIN_TH=0.00 PRIMER_INTERNAL_44_PENALTY=6.889273 PRIMER_INTERNAL_44_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_44=114,26 PRIMER_INTERNAL_44_TM=59.111 PRIMER_INTERNAL_44_GC_PERCENT=46.154 PRIMER_INTERNAL_44_SELF_ANY_TH=0.00 PRIMER_INTERNAL_44_SELF_END_TH=0.00 PRIMER_INTERNAL_44_HAIRPIN_TH=0.00 PRIMER_INTERNAL_45_PENALTY=6.889273 PRIMER_INTERNAL_45_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_45=113,26 PRIMER_INTERNAL_45_TM=59.111 PRIMER_INTERNAL_45_GC_PERCENT=46.154 PRIMER_INTERNAL_45_SELF_ANY_TH=0.00 PRIMER_INTERNAL_45_SELF_END_TH=0.00 PRIMER_INTERNAL_45_HAIRPIN_TH=0.00 PRIMER_INTERNAL_46_PENALTY=6.910113 PRIMER_INTERNAL_46_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGA PRIMER_INTERNAL_46=121,24 PRIMER_INTERNAL_46_TM=62.910 PRIMER_INTERNAL_46_GC_PERCENT=58.333 PRIMER_INTERNAL_46_SELF_ANY_TH=7.82 PRIMER_INTERNAL_46_SELF_END_TH=0.00 PRIMER_INTERNAL_46_HAIRPIN_TH=36.42 PRIMER_INTERNAL_47_PENALTY=6.910113 PRIMER_INTERNAL_47_SEQUENCE=TCCAAAACGCAAAGCACGCTCCCG PRIMER_INTERNAL_47=120,24 PRIMER_INTERNAL_47_TM=62.910 PRIMER_INTERNAL_47_GC_PERCENT=58.333 PRIMER_INTERNAL_47_SELF_ANY_TH=1.50 PRIMER_INTERNAL_47_SELF_END_TH=1.50 PRIMER_INTERNAL_47_HAIRPIN_TH=41.14 PRIMER_INTERNAL_48_PENALTY=7.008353 PRIMER_INTERNAL_48_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_48=116,25 PRIMER_INTERNAL_48_TM=57.992 PRIMER_INTERNAL_48_GC_PERCENT=48.000 PRIMER_INTERNAL_48_SELF_ANY_TH=0.00 PRIMER_INTERNAL_48_SELF_END_TH=0.00 PRIMER_INTERNAL_48_HAIRPIN_TH=0.00 PRIMER_INTERNAL_49_PENALTY=7.035358 PRIMER_INTERNAL_49_SEQUENCE=AGTCTATTCCAAAACGCAAAGCACGCT PRIMER_INTERNAL_49=113,27 PRIMER_INTERNAL_49_TM=59.965 PRIMER_INTERNAL_49_GC_PERCENT=44.444 PRIMER_INTERNAL_49_SELF_ANY_TH=0.00 PRIMER_INTERNAL_49_SELF_END_TH=0.00 PRIMER_INTERNAL_49_HAIRPIN_TH=0.00 PRIMER_INTERNAL_50_PENALTY=7.102160 PRIMER_INTERNAL_50_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_50=115,26 PRIMER_INTERNAL_50_TM=58.898 PRIMER_INTERNAL_50_GC_PERCENT=46.154 PRIMER_INTERNAL_50_SELF_ANY_TH=0.00 PRIMER_INTERNAL_50_SELF_END_TH=0.00 PRIMER_INTERNAL_50_HAIRPIN_TH=0.00 PRIMER_INTERNAL_51_PENALTY=7.334450 PRIMER_INTERNAL_51_SEQUENCE=GTCTATTCCAAAACGCAAAGCACGCTC PRIMER_INTERNAL_51=114,27 PRIMER_INTERNAL_51_TM=59.666 PRIMER_INTERNAL_51_GC_PERCENT=48.148 PRIMER_INTERNAL_51_SELF_ANY_TH=0.00 PRIMER_INTERNAL_51_SELF_END_TH=0.00 PRIMER_INTERNAL_51_HAIRPIN_TH=0.00 PRIMER_INTERNAL_52_PENALTY=7.480679 PRIMER_INTERNAL_52_SEQUENCE=TCTATTCCAAAACGCAAAGCACGCTCC PRIMER_INTERNAL_52=115,27 PRIMER_INTERNAL_52_TM=60.481 PRIMER_INTERNAL_52_GC_PERCENT=48.148 PRIMER_INTERNAL_52_SELF_ANY_TH=0.00 PRIMER_INTERNAL_52_SELF_END_TH=0.00 PRIMER_INTERNAL_52_HAIRPIN_TH=0.00 PRIMER_INTERNAL_53_PENALTY=7.647244 PRIMER_INTERNAL_53_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACGC PRIMER_INTERNAL_53=112,27 PRIMER_INTERNAL_53_TM=59.353 PRIMER_INTERNAL_53_GC_PERCENT=44.444 PRIMER_INTERNAL_53_SELF_ANY_TH=0.00 PRIMER_INTERNAL_53_SELF_END_TH=0.00 PRIMER_INTERNAL_53_HAIRPIN_TH=0.00 PRIMER_INTERNAL_54_PENALTY=7.744113 PRIMER_INTERNAL_54_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGT PRIMER_INTERNAL_54=55,26 PRIMER_INTERNAL_54_TM=58.256 PRIMER_INTERNAL_54_GC_PERCENT=46.154 PRIMER_INTERNAL_54_SELF_ANY_TH=0.00 PRIMER_INTERNAL_54_SELF_END_TH=0.00 PRIMER_INTERNAL_54_HAIRPIN_TH=0.00 PRIMER_INTERNAL_55_PENALTY=7.867066 PRIMER_INTERNAL_55_SEQUENCE=CCAAAACGCAAAGCACGCTCCCGAT PRIMER_INTERNAL_55=121,25 PRIMER_INTERNAL_55_TM=62.867 PRIMER_INTERNAL_55_GC_PERCENT=56.000 PRIMER_INTERNAL_55_SELF_ANY_TH=7.82 PRIMER_INTERNAL_55_SELF_END_TH=0.00 PRIMER_INTERNAL_55_HAIRPIN_TH=36.42 PRIMER_INTERNAL_56_PENALTY=7.916609 PRIMER_INTERNAL_56_SEQUENCE=TGACCTGATAGAAACGCCAACTCAC PRIMER_INTERNAL_56=53,25 PRIMER_INTERNAL_56_TM=57.083 PRIMER_INTERNAL_56_GC_PERCENT=48.000 PRIMER_INTERNAL_56_SELF_ANY_TH=0.00 PRIMER_INTERNAL_56_SELF_END_TH=0.00 PRIMER_INTERNAL_56_HAIRPIN_TH=0.00 PRIMER_INTERNAL_57_PENALTY=7.922893 PRIMER_INTERNAL_57_SEQUENCE=CCTGATAGAAACGCCAACTCACTGT PRIMER_INTERNAL_57=56,25 PRIMER_INTERNAL_57_TM=57.077 PRIMER_INTERNAL_57_GC_PERCENT=48.000 PRIMER_INTERNAL_57_SELF_ANY_TH=0.00 PRIMER_INTERNAL_57_SELF_END_TH=0.00 PRIMER_INTERNAL_57_HAIRPIN_TH=0.00 PRIMER_INTERNAL_58_PENALTY=7.922893 PRIMER_INTERNAL_58_SEQUENCE=ACCTGATAGAAACGCCAACTCACTG PRIMER_INTERNAL_58=55,25 PRIMER_INTERNAL_58_TM=57.077 PRIMER_INTERNAL_58_GC_PERCENT=48.000 PRIMER_INTERNAL_58_SELF_ANY_TH=0.00 PRIMER_INTERNAL_58_SELF_END_TH=0.00 PRIMER_INTERNAL_58_HAIRPIN_TH=0.00 PRIMER_INTERNAL_59_PENALTY=7.963894 PRIMER_INTERNAL_59_SEQUENCE=TGACCTGATAGAAACGCCAACTCACT PRIMER_INTERNAL_59=53,26 PRIMER_INTERNAL_59_TM=58.036 PRIMER_INTERNAL_59_GC_PERCENT=46.154 PRIMER_INTERNAL_59_SELF_ANY_TH=0.00 PRIMER_INTERNAL_59_SELF_END_TH=0.00 PRIMER_INTERNAL_59_HAIRPIN_TH=0.00 PRIMER_INTERNAL_60_PENALTY=8.131843 PRIMER_INTERNAL_60_SEQUENCE=TGACCTGATAGAAACGCCAACTCACTG PRIMER_INTERNAL_60=53,27 PRIMER_INTERNAL_60_TM=58.868 PRIMER_INTERNAL_60_GC_PERCENT=48.148 PRIMER_INTERNAL_60_SELF_ANY_TH=0.00 PRIMER_INTERNAL_60_SELF_END_TH=0.00 PRIMER_INTERNAL_60_HAIRPIN_TH=0.00 PRIMER_INTERNAL_61_PENALTY=8.132460 PRIMER_INTERNAL_61_SEQUENCE=GACCTGATAGAAACGCCAACTCACTGT PRIMER_INTERNAL_61=54,27 PRIMER_INTERNAL_61_TM=58.868 PRIMER_INTERNAL_61_GC_PERCENT=48.148 PRIMER_INTERNAL_61_SELF_ANY_TH=0.00 PRIMER_INTERNAL_61_SELF_END_TH=0.00 PRIMER_INTERNAL_61_HAIRPIN_TH=0.00 PRIMER_INTERNAL_62_PENALTY=8.193320 PRIMER_INTERNAL_62_SEQUENCE=CTATTCCAAAACGCAAAGCACGCTCCC PRIMER_INTERNAL_62=116,27 PRIMER_INTERNAL_62_TM=61.193 PRIMER_INTERNAL_62_GC_PERCENT=51.852 PRIMER_INTERNAL_62_SELF_ANY_TH=0.00 PRIMER_INTERNAL_62_SELF_END_TH=0.00 PRIMER_INTERNAL_62_HAIRPIN_TH=0.00 PRIMER_INTERNAL_63_PENALTY=8.237701 PRIMER_INTERNAL_63_SEQUENCE=GACCTGATAGAAACGCCAACTCACTG PRIMER_INTERNAL_63=54,26 PRIMER_INTERNAL_63_TM=57.762 PRIMER_INTERNAL_63_GC_PERCENT=50.000 PRIMER_INTERNAL_63_SELF_ANY_TH=0.00 PRIMER_INTERNAL_63_SELF_END_TH=0.00 PRIMER_INTERNAL_63_HAIRPIN_TH=0.00 PRIMER_INTERNAL_64_PENALTY=8.755267 PRIMER_INTERNAL_64_SEQUENCE=ATGACCTGATAGAAACGCCAACTCAC PRIMER_INTERNAL_64=52,26 PRIMER_INTERNAL_64_TM=57.245 PRIMER_INTERNAL_64_GC_PERCENT=46.154 PRIMER_INTERNAL_64_SELF_ANY_TH=0.00 PRIMER_INTERNAL_64_SELF_END_TH=0.00 PRIMER_INTERNAL_64_HAIRPIN_TH=0.00 PRIMER_INTERNAL_65_PENALTY=8.758760 PRIMER_INTERNAL_65_SEQUENCE=AAGTCTATTCCAAAACGCAAAGCACG PRIMER_INTERNAL_65=112,26 PRIMER_INTERNAL_65_TM=57.241 PRIMER_INTERNAL_65_GC_PERCENT=42.308 PRIMER_INTERNAL_65_SELF_ANY_TH=0.00 PRIMER_INTERNAL_65_SELF_END_TH=0.00 PRIMER_INTERNAL_65_HAIRPIN_TH=0.00 PRIMER_INTERNAL_66_PENALTY=8.838967 PRIMER_INTERNAL_66_SEQUENCE=ATGACCTGATAGAAACGCCAACTCACT PRIMER_INTERNAL_66=52,27 PRIMER_INTERNAL_66_TM=58.161 PRIMER_INTERNAL_66_GC_PERCENT=44.444 PRIMER_INTERNAL_66_SELF_ANY_TH=0.00 PRIMER_INTERNAL_66_SELF_END_TH=0.00 PRIMER_INTERNAL_66_HAIRPIN_TH=0.00 PRIMER_INTERNAL_67_PENALTY=8.978186 PRIMER_INTERNAL_67_SEQUENCE=ATTCCAAAACGCAAAGCACGCTCCCG PRIMER_INTERNAL_67=118,26 PRIMER_INTERNAL_67_TM=62.978 PRIMER_INTERNAL_67_GC_PERCENT=53.846 PRIMER_INTERNAL_67_SELF_ANY_TH=1.50 PRIMER_INTERNAL_67_SELF_END_TH=1.50 PRIMER_INTERNAL_67_HAIRPIN_TH=41.14 PRIMER_INTERNAL_68_PENALTY=9.099564 PRIMER_INTERNAL_68_SEQUENCE=ACCTGATAGAAACGCCAACTCACTGTA PRIMER_INTERNAL_68=55,27 PRIMER_INTERNAL_68_TM=57.900 PRIMER_INTERNAL_68_GC_PERCENT=44.444 PRIMER_INTERNAL_68_SELF_ANY_TH=0.00 PRIMER_INTERNAL_68_SELF_END_TH=0.00 PRIMER_INTERNAL_68_HAIRPIN_TH=0.00 PRIMER_INTERNAL_69_PENALTY=9.448747 PRIMER_INTERNAL_69_SEQUENCE=AAAGTCTATTCCAAAACGCAAAGCACG PRIMER_INTERNAL_69=111,27 PRIMER_INTERNAL_69_TM=57.551 PRIMER_INTERNAL_69_GC_PERCENT=40.741 PRIMER_INTERNAL_69_SELF_ANY_TH=0.00 PRIMER_INTERNAL_69_SELF_END_TH=0.00 PRIMER_INTERNAL_69_HAIRPIN_TH=0.00 PRIMER_INTERNAL_70_PENALTY=9.472089 PRIMER_INTERNAL_70_SEQUENCE=TATTCCAAAACGCAAAGCACGCTCCCG PRIMER_INTERNAL_70=117,27 PRIMER_INTERNAL_70_TM=62.472 PRIMER_INTERNAL_70_GC_PERCENT=51.852 PRIMER_INTERNAL_70_SELF_ANY_TH=1.50 PRIMER_INTERNAL_70_SELF_END_TH=1.50 PRIMER_INTERNAL_70_HAIRPIN_TH=41.14 PRIMER_INTERNAL_71_PENALTY=9.912698 PRIMER_INTERNAL_71_SEQUENCE=CGTTGTCAAAGTCTATTCCAAAACGCA PRIMER_INTERNAL_71=104,27 PRIMER_INTERNAL_71_TM=57.087 PRIMER_INTERNAL_71_GC_PERCENT=40.741 PRIMER_INTERNAL_71_SELF_ANY_TH=0.00 PRIMER_INTERNAL_71_SELF_END_TH=0.00 PRIMER_INTERNAL_71_HAIRPIN_TH=0.00 PRIMER_INTERNAL_72_PENALTY=9.914131 PRIMER_INTERNAL_72_SEQUENCE=ACGTTGTCAAAGTCTATTCCAAAACGC PRIMER_INTERNAL_72=103,27 PRIMER_INTERNAL_72_TM=57.086 PRIMER_INTERNAL_72_GC_PERCENT=40.741 PRIMER_INTERNAL_72_SELF_ANY_TH=0.00 PRIMER_INTERNAL_72_SELF_END_TH=0.00 PRIMER_INTERNAL_72_HAIRPIN_TH=0.00 = PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_LEFT_PRIMER=0 PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_NUM_RETURN=1 P3_FILE_FLAG=0 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1 PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_sequencing_primers_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_ID=pick_sequencing_primers_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_WARNING=No left primer found in range 1 - 40 PRIMER_LEFT_EXPLAIN=considered 56, too many Ns 5, low tm 51, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_RIGHT_PRIMER=1 SEQUENCE_ID=pick_sequencing_primers_th_3 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_WARNING=No left primer found in range 1 - 40 PRIMER_LEFT_EXPLAIN=considered 56, too many Ns 5, low tm 51, ok 0 PRIMER_RIGHT_EXPLAIN=considered 63, low tm 33, high tm 13, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.486439 PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAT PRIMER_RIGHT_0=137,20 PRIMER_RIGHT_0_TM=58.514 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse MER46 Nonautonomous DNA transposon PRIMER_RIGHT_0_END_STABILITY=3.0100 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=9.0760 = PRIMER_TASK=check_primers PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=check_primers_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 SEQUENCE_PRIMER=GACATGACACTGACGATGCA SEQUENCE_PRIMER_REVCOMP=TAGCTATCGATTTGGGTCGG PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, low tm 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=check_primers_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC SEQUENCE_PRIMER=TGACCTGATAGAAACGCCAA SEQUENCE_PRIMER_REVCOMP=TGCTTTGCGTTTTGGAATAG PRIMER_LEFT_EXPLAIN=considered 1, low tm 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 1, low tm 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = PRIMER_TASK=pick_discriminative_primers PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_disc_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 3, overlap target 3, low tm 2, ok 3 PRIMER_RIGHT_EXPLAIN=considered 3, overlap target 3, low tm 1, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_disc_primer_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC SEQUENCE_TARGET=37,21 PRIMER_LEFT_EXPLAIN=considered 3, overlap target 3, low tm 3, ok 3 PRIMER_RIGHT_EXPLAIN=considered 3, overlap target 3, low tm 2, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = PRIMER_TASK=pick_cloning_primers PRIMER_PRODUCT_SIZE_RANGE=200-300 SEQUENCE_ID=pick_disc_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=2,100 PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = PRIMER_PRODUCT_SIZE_RANGE=200-300 SEQUENCE_ID=pick_disc_primer_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC SEQUENCE_TARGET=2,120 PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = primer3-2.3.6/test/p3_3_prime_n_input0000664000077300007730000001425412231156245016462 0ustar ioanaioanaPRIMER_PRODUCT_SIZE_RANGE=100-500 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_NUM_RETURN=40 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_THREE_PRIME_DISTANCE=-1 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=0 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=1 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=2 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=3 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=4 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=5 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=6 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=7 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=8 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=9 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=10 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=11 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=12 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=13 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=14 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=15 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=16 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=17 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=18 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=19 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=20 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=21 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = PRIMER_MIN_THREE_PRIME_DISTANCE=22 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = P3_COMMENT='should complain' PRIMER_MIN_THREE_PRIME_DISTANCE=-2 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA = primer3-2.3.6/test/primer_human_output0000664000077300007730000552221112231156245017076 0ustar ioanaioanaPRIMER_OPT_SIZE=20 PRIMER_PICK_INTERNAL_OLIGO=1 P3_COMMENT=Used to have: PRIMER_INTERNAL_OLIGO_OFFSET 4 (not used) PRIMER_INTERNAL_MAX_SELF_END=8 PRIMER_MIN_SIZE=18 PRIMER_MAX_SIZE=25 PRIMER_OPT_TM=60.0 PRIMER_MIN_TM=57.0 PRIMER_MAX_TM=63.0 PRIMER_MIN_GC=20.0 PRIMER_MAX_GC=80.0 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_SALT_MONOVALENT=50.0 PRIMER_DNA_CONC=50.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=8 P3_COMMENT= Used to have: PRIMER_NUM_TERMINATORS_0 (not used) P3_COMMENT= Used to have: PRIMER_REST_SITES_" " (not used) P3_COMMENT= Used to have: PRIMER_ALLOW_REST_SITE_0 (not used) PRIMER_PRODUCT_SIZE_RANGE=75-100 100-125 125-150 150-175 175-200 200-225 SEQUENCE_ID=MH1000 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTNAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG SEQUENCE_INCLUDED_REGION=36,342 P3_COMMENT=CL is TTACTGGAAGAGAGGGTCATG P3_COMMENT=CR is AGGGAAACATCTGCAAGGTT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.150428 PRIMER_LEFT_0_PENALTY=0.742026 PRIMER_RIGHT_0_PENALTY=0.408401 PRIMER_INTERNAL_0_PENALTY=0.017846 PRIMER_LEFT_0_SEQUENCE=ACTGGAAGAGAGGGTCATGC PRIMER_RIGHT_0_SEQUENCE=CATTCAGTGCGTCTCCTCCT PRIMER_INTERNAL_0_SEQUENCE=AAGTTTGCAAAGGCAGGCTA PRIMER_LEFT_0=69,20 PRIMER_RIGHT_0=143,20 PRIMER_INTERNAL_0=104,20 PRIMER_LEFT_0_TM=59.258 PRIMER_RIGHT_0_TM=60.408 PRIMER_INTERNAL_0_TM=60.018 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=1.186960 PRIMER_LEFT_1_PENALTY=0.339231 PRIMER_RIGHT_1_PENALTY=0.847729 PRIMER_INTERNAL_1_PENALTY=0.017846 PRIMER_LEFT_1_SEQUENCE=GGAAGAGAGGGTCATGCAAC PRIMER_RIGHT_1_SEQUENCE=CTTACCATGGCATTCAGTGC PRIMER_INTERNAL_1_SEQUENCE=GTTTGCAAAGGCAGGCTAAG PRIMER_LEFT_1=72,20 PRIMER_RIGHT_1=153,20 PRIMER_INTERNAL_1=106,20 PRIMER_LEFT_1_TM=59.661 PRIMER_RIGHT_1_TM=59.152 PRIMER_INTERNAL_1_TM=60.018 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=1.526945 PRIMER_LEFT_2_PENALTY=1.509099 PRIMER_RIGHT_2_PENALTY=0.017846 PRIMER_INTERNAL_2_PENALTY=0.339231 PRIMER_LEFT_2_SEQUENCE=GCATCAGTGAGTACAGCATGC PRIMER_RIGHT_2_SEQUENCE=AGCCTGCCTTTGCAAACTTA PRIMER_INTERNAL_2_SEQUENCE=GGAAGAGAGGGTCATGCAAC PRIMER_LEFT_2=46,21 PRIMER_RIGHT_2=122,20 PRIMER_INTERNAL_2=72,20 PRIMER_LEFT_2_TM=59.491 PRIMER_RIGHT_2_TM=60.018 PRIMER_INTERNAL_2_TM=59.661 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=5.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=1.526945 PRIMER_LEFT_3_PENALTY=1.509099 PRIMER_RIGHT_3_PENALTY=0.017846 PRIMER_INTERNAL_3_PENALTY=0.339231 PRIMER_LEFT_3_SEQUENCE=GCATCAGTGAGTACAGCATGC PRIMER_RIGHT_3_SEQUENCE=TAGCCTGCCTTTGCAAACTT PRIMER_INTERNAL_3_SEQUENCE=GGAAGAGAGGGTCATGCAAC PRIMER_LEFT_3=46,21 PRIMER_RIGHT_3=123,20 PRIMER_INTERNAL_3=72,20 PRIMER_LEFT_3_TM=59.491 PRIMER_RIGHT_3_TM=60.018 PRIMER_INTERNAL_3_TM=59.661 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=78 PRIMER_PAIR_4_PENALTY=1.526945 PRIMER_LEFT_4_PENALTY=1.509099 PRIMER_RIGHT_4_PENALTY=0.017846 PRIMER_INTERNAL_4_PENALTY=0.339231 PRIMER_LEFT_4_SEQUENCE=GCATCAGTGAGTACAGCATGC PRIMER_RIGHT_4_SEQUENCE=TTAGCCTGCCTTTGCAAACT PRIMER_INTERNAL_4_SEQUENCE=GGAAGAGAGGGTCATGCAAC PRIMER_LEFT_4=46,21 PRIMER_RIGHT_4=124,20 PRIMER_INTERNAL_4=72,20 PRIMER_LEFT_4_TM=59.491 PRIMER_RIGHT_4_TM=60.018 PRIMER_INTERNAL_4_TM=59.661 PRIMER_LEFT_4_GC_PERCENT=52.381 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH101 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGATCATCTTCTGATCCAAGACCCGTGCTAGTNCTACTGTATTCTACATGTACCCTTCTACAAGCTTTGAATTCCATAATCATCAGAGAATCCAGAAATTATTCATTATCACATAATAACAACACTCAAATATTTAAAGTTTTATGGTTGAATCTTTATGATTGAACCCATTCTATCAGGTGGTTATTTTATATCTAATAGAATTCATTTCAGGGTTACACAAAGGTCCGAATCTATCCATTTTACTGAG SEQUENCE_INCLUDED_REGION=35,254 P3_COMMENT=CL is CCTCTGATCATCTTCTGATCC P3_COMMENT=CR is GTAAAATGGATAGATTCGGACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.588776 PRIMER_LEFT_0_PENALTY=1.201261 PRIMER_RIGHT_0_PENALTY=2.387515 PRIMER_INTERNAL_0_PENALTY=8.314301 PRIMER_LEFT_0_SEQUENCE=TGAACCCATTCTATCAGGTGG PRIMER_RIGHT_0_SEQUENCE=TGGATAGATTCGGACCTTTG PRIMER_INTERNAL_0_SEQUENCE=TCTAATAGAATTCATTTCAGGGTTACA PRIMER_LEFT_0=201,21 PRIMER_RIGHT_0=278,20 PRIMER_INTERNAL_0=232,27 PRIMER_LEFT_0_TM=59.799 PRIMER_RIGHT_0_TM=57.612 PRIMER_INTERNAL_0_TM=58.686 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=29.630 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 PRIMER_PAIR_1_PENALTY=3.868652 PRIMER_LEFT_1_PENALTY=1.201261 PRIMER_RIGHT_1_PENALTY=2.667391 PRIMER_INTERNAL_1_PENALTY=8.314301 PRIMER_LEFT_1_SEQUENCE=TGAACCCATTCTATCAGGTGG PRIMER_RIGHT_1_SEQUENCE=AATGGATAGATTCGGACCTTTG PRIMER_INTERNAL_1_SEQUENCE=TCTAATAGAATTCATTTCAGGGTTACA PRIMER_LEFT_1=201,21 PRIMER_RIGHT_1=280,22 PRIMER_INTERNAL_1=232,27 PRIMER_LEFT_1_TM=59.799 PRIMER_RIGHT_1_TM=59.333 PRIMER_INTERNAL_1_TM=58.686 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_INTERNAL_1_GC_PERCENT=29.630 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.3000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=4.195249 PRIMER_LEFT_2_PENALTY=1.201261 PRIMER_RIGHT_2_PENALTY=2.993987 PRIMER_INTERNAL_2_PENALTY=8.314301 PRIMER_LEFT_2_SEQUENCE=TGAACCCATTCTATCAGGTGG PRIMER_RIGHT_2_SEQUENCE=ATGGATAGATTCGGACCTTTG PRIMER_INTERNAL_2_SEQUENCE=TCTAATAGAATTCATTTCAGGGTTACA PRIMER_LEFT_2=201,21 PRIMER_RIGHT_2=279,21 PRIMER_INTERNAL_2=232,27 PRIMER_LEFT_2_TM=59.799 PRIMER_RIGHT_2_TM=58.006 PRIMER_INTERNAL_2_TM=58.686 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=29.630 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=7.3000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=79 PRIMER_PAIR_3_PENALTY=4.414739 PRIMER_LEFT_3_PENALTY=0.345214 PRIMER_RIGHT_3_PENALTY=4.069526 PRIMER_INTERNAL_3_PENALTY=4.276719 PRIMER_LEFT_3_SEQUENCE=TCTTCTGATCCAAGACCCGT PRIMER_RIGHT_3_SEQUENCE=TCTGGATTCTCTGATGATTATGGA PRIMER_INTERNAL_3_SEQUENCE=CATGTACCCTTCTACAAGCTTTGA PRIMER_LEFT_3=45,20 PRIMER_RIGHT_3=134,24 PRIMER_INTERNAL_3=85,24 PRIMER_LEFT_3_TM=59.655 PRIMER_RIGHT_3_TM=59.930 PRIMER_INTERNAL_3_TM=59.723 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=37.500 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=11.1000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=4.715860 PRIMER_LEFT_4_PENALTY=0.646334 PRIMER_RIGHT_4_PENALTY=4.069526 PRIMER_INTERNAL_4_PENALTY=4.276719 PRIMER_LEFT_4_SEQUENCE=CTTCTGATCCAAGACCCGTG PRIMER_RIGHT_4_SEQUENCE=TCTGGATTCTCTGATGATTATGGA PRIMER_INTERNAL_4_SEQUENCE=CATGTACCCTTCTACAAGCTTTGA PRIMER_LEFT_4=46,20 PRIMER_RIGHT_4=134,24 PRIMER_INTERNAL_4=85,24 PRIMER_LEFT_4_TM=60.646 PRIMER_RIGHT_4_TM=59.930 PRIMER_INTERNAL_4_TM=59.723 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=37.500 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=9.9000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH1013 SEQUENCE_TEMPLATE=CTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGTGGAGAAGCCTGGTCAGGGAGAGTATACAGGGATGGGGACTGCATGTATGGTTTGGAGTNCAGGAGGGAGAAAGGTGGTTCTTGTGGGAAGAGGTCATCTTCTAGGGCTGAGTTTGGCATTCTTAAGTTTTAACTCTGGGCCTGATATTCCCTGTTAGCCATCTCCAGGAAGAAGCATTTCATGACTCATATTTTTAACATGACTAATGTTACCTAGAACCACGTATCCTCC SEQUENCE_INCLUDED_REGION=33,235 P3_COMMENT=CL is CTGGTCAGGGAGAGTATACAGG P3_COMMENT=CR is GTGGTTCTAGGTAACATTAGTCATG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.244226 PRIMER_LEFT_0_PENALTY=0.080568 PRIMER_RIGHT_0_PENALTY=0.163658 PRIMER_INTERNAL_0_PENALTY=0.056936 PRIMER_LEFT_0_SEQUENCE=ATGGGGACTGCATGTATGGT PRIMER_RIGHT_0_SEQUENCE=ATGCCAAACTCAGCCCTAGA PRIMER_INTERNAL_0_SEQUENCE=GAAAGGTGGTTCTTGTGGGA PRIMER_LEFT_0=68,20 PRIMER_RIGHT_0=155,20 PRIMER_INTERNAL_0=105,20 PRIMER_LEFT_0_TM=60.081 PRIMER_RIGHT_0_TM=59.836 PRIMER_INTERNAL_0_TM=59.943 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.8000 PRIMER_RIGHT_0_END_STABILITY=5.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 PRIMER_PAIR_1_PENALTY=0.266806 PRIMER_LEFT_1_PENALTY=0.056936 PRIMER_RIGHT_1_PENALTY=0.209870 PRIMER_INTERNAL_1_PENALTY=0.163658 PRIMER_LEFT_1_SEQUENCE=GAAAGGTGGTTCTTGTGGGA PRIMER_RIGHT_1_SEQUENCE=CTGGAGATGGCTAACAGGGA PRIMER_INTERNAL_1_SEQUENCE=TCTAGGGCTGAGTTTGGCAT PRIMER_LEFT_1=105,20 PRIMER_RIGHT_1=203,20 PRIMER_INTERNAL_1=136,20 PRIMER_LEFT_1_TM=59.943 PRIMER_RIGHT_1_TM=60.210 PRIMER_INTERNAL_1_TM=59.836 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=0.294074 PRIMER_LEFT_2_PENALTY=0.080568 PRIMER_RIGHT_2_PENALTY=0.213506 PRIMER_INTERNAL_2_PENALTY=0.056936 PRIMER_LEFT_2_SEQUENCE=ATGGGGACTGCATGTATGGT PRIMER_RIGHT_2_SEQUENCE=GAATGCCAAACTCAGCCCTA PRIMER_INTERNAL_2_SEQUENCE=GAAAGGTGGTTCTTGTGGGA PRIMER_LEFT_2=68,20 PRIMER_RIGHT_2=157,20 PRIMER_INTERNAL_2=105,20 PRIMER_LEFT_2_TM=60.081 PRIMER_RIGHT_2_TM=60.214 PRIMER_INTERNAL_2_TM=59.943 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.8000 PRIMER_RIGHT_2_END_STABILITY=8.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=0.350010 PRIMER_LEFT_3_PENALTY=0.056936 PRIMER_RIGHT_3_PENALTY=0.293074 PRIMER_INTERNAL_3_PENALTY=0.163658 PRIMER_LEFT_3_SEQUENCE=GAAAGGTGGTTCTTGTGGGA PRIMER_RIGHT_3_SEQUENCE=GGGAATATCAGGCCCAGAGT PRIMER_INTERNAL_3_SEQUENCE=TCTAGGGCTGAGTTTGGCAT PRIMER_LEFT_3=105,20 PRIMER_RIGHT_3=187,20 PRIMER_INTERNAL_3=136,20 PRIMER_LEFT_3_TM=59.943 PRIMER_RIGHT_3_TM=60.293 PRIMER_INTERNAL_3_TM=59.836 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=0.390277 PRIMER_LEFT_4_PENALTY=0.226618 PRIMER_RIGHT_4_PENALTY=0.163658 PRIMER_INTERNAL_4_PENALTY=0.056936 PRIMER_LEFT_4_SEQUENCE=TATACAGGGATGGGGACTGC PRIMER_RIGHT_4_SEQUENCE=ATGCCAAACTCAGCCCTAGA PRIMER_INTERNAL_4_SEQUENCE=GAAAGGTGGTTCTTGTGGGA PRIMER_LEFT_4=59,20 PRIMER_RIGHT_4=155,20 PRIMER_INTERNAL_4=105,20 PRIMER_LEFT_4_TM=59.773 PRIMER_RIGHT_4_TM=59.836 PRIMER_INTERNAL_4_TM=59.943 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=5.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=97 = SEQUENCE_ID=MH1017 SEQUENCE_TEMPLATE=TGTTTCATGCTGCAGGTCGACTCTAGAGGATCCCTACATAAACAGCAGCGAAAGGAGAAAGCTTTAGGGCAGAAGCAGGACTGGGCCTGTGGNATCTATAGGTAAGATGAATCCCTTTGGGGAAGACCTTGAATAAGAGATTGAAAGGAAGAGTGGGGTCTATCATTTGCAAAG SEQUENCE_INCLUDED_REGION=34,140 P3_COMMENT=CL is ACATAAACAGCAGCGAAAGG P3_COMMENT=CR is TGCAAATGATAGACCCCACT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.764198 PRIMER_LEFT_0_PENALTY=0.132262 PRIMER_RIGHT_0_PENALTY=0.631936 PRIMER_INTERNAL_0_PENALTY=0.528861 PRIMER_LEFT_0_SEQUENCE=AACAGCAGCGAAAGGAGAAA PRIMER_RIGHT_0_SEQUENCE=TCCCCAAAGGGATTCATCTT PRIMER_INTERNAL_0_SEQUENCE=TTAGGGCAGAAGCAGGACTG PRIMER_LEFT_0=40,20 PRIMER_RIGHT_0=122,20 PRIMER_INTERNAL_0=63,20 PRIMER_LEFT_0_TM=60.132 PRIMER_RIGHT_0_TM=60.632 PRIMER_INTERNAL_0_TM=60.529 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=83 PRIMER_PAIR_1_PENALTY=0.764198 PRIMER_LEFT_1_PENALTY=0.132262 PRIMER_RIGHT_1_PENALTY=0.631936 PRIMER_INTERNAL_1_PENALTY=0.528861 PRIMER_LEFT_1_SEQUENCE=AACAGCAGCGAAAGGAGAAA PRIMER_RIGHT_1_SEQUENCE=TTCCCCAAAGGGATTCATCT PRIMER_INTERNAL_1_SEQUENCE=TTAGGGCAGAAGCAGGACTG PRIMER_LEFT_1=40,20 PRIMER_RIGHT_1=123,20 PRIMER_INTERNAL_1=63,20 PRIMER_LEFT_1_TM=60.132 PRIMER_RIGHT_1_TM=60.632 PRIMER_INTERNAL_1_TM=60.529 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=84 PRIMER_PAIR_2_PENALTY=0.764198 PRIMER_LEFT_2_PENALTY=0.132262 PRIMER_RIGHT_2_PENALTY=0.631936 PRIMER_INTERNAL_2_PENALTY=0.528861 PRIMER_LEFT_2_SEQUENCE=AACAGCAGCGAAAGGAGAAA PRIMER_RIGHT_2_SEQUENCE=CTTCCCCAAAGGGATTCATC PRIMER_INTERNAL_2_SEQUENCE=TTAGGGCAGAAGCAGGACTG PRIMER_LEFT_2=40,20 PRIMER_RIGHT_2=124,20 PRIMER_INTERNAL_2=63,20 PRIMER_LEFT_2_TM=60.132 PRIMER_RIGHT_2_TM=60.632 PRIMER_INTERNAL_2_TM=60.529 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=85 PRIMER_PAIR_3_PENALTY=0.764198 PRIMER_LEFT_3_PENALTY=0.132262 PRIMER_RIGHT_3_PENALTY=0.631936 PRIMER_INTERNAL_3_PENALTY=0.528861 PRIMER_LEFT_3_SEQUENCE=AACAGCAGCGAAAGGAGAAA PRIMER_RIGHT_3_SEQUENCE=TCTTCCCCAAAGGGATTCAT PRIMER_INTERNAL_3_SEQUENCE=TTAGGGCAGAAGCAGGACTG PRIMER_LEFT_3=40,20 PRIMER_RIGHT_3=125,20 PRIMER_INTERNAL_3=63,20 PRIMER_LEFT_3_TM=60.132 PRIMER_RIGHT_3_TM=60.632 PRIMER_INTERNAL_3_TM=60.529 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=86 PRIMER_PAIR_4_PENALTY=0.764198 PRIMER_LEFT_4_PENALTY=0.132262 PRIMER_RIGHT_4_PENALTY=0.631936 PRIMER_INTERNAL_4_PENALTY=0.528861 PRIMER_LEFT_4_SEQUENCE=AAACAGCAGCGAAAGGAGAA PRIMER_RIGHT_4_SEQUENCE=TCCCCAAAGGGATTCATCTT PRIMER_INTERNAL_4_SEQUENCE=TTAGGGCAGAAGCAGGACTG PRIMER_LEFT_4=39,20 PRIMER_RIGHT_4=122,20 PRIMER_INTERNAL_4=63,20 PRIMER_LEFT_4_TM=60.132 PRIMER_RIGHT_4_TM=60.632 PRIMER_INTERNAL_4_TM=60.529 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=84 = SEQUENCE_ID=MH1020 SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTCCCAAGATCACAGGTCAAGACTTCTACTATCAGGAGACTCATGTCTTTGAATATTTTTCTTCTTTATGCCTCTATGAACAATAGAAATGAAAAGGGGATCTGTTGTATACACTCACAAGGTATACTTTTATTTGTGAAGGATTTTTCAG SEQUENCE_INCLUDED_REGION=33,154 P3_COMMENT=CL is TTTTCCCAAGATCACAGGTC P3_COMMENT=CR is CCTTCACAAATAAAAGTATACCTTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.558607 PRIMER_LEFT_0_PENALTY=0.940718 PRIMER_RIGHT_0_PENALTY=3.617890 PRIMER_INTERNAL_0_PENALTY=4.485568 PRIMER_LEFT_0_SEQUENCE=TTCCCAAGATCACAGGTCAA PRIMER_RIGHT_0_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA PRIMER_INTERNAL_0_SEQUENCE=TCAGGAGACTCATGTCTTTGAA PRIMER_LEFT_0=36,20 PRIMER_RIGHT_0=134,23 PRIMER_INTERNAL_0=67,22 PRIMER_LEFT_0_TM=59.059 PRIMER_RIGHT_0_TM=59.382 PRIMER_INTERNAL_0_TM=57.514 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=34.783 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=99 PRIMER_PAIR_1_PENALTY=4.558607 PRIMER_LEFT_1_PENALTY=0.940718 PRIMER_RIGHT_1_PENALTY=3.617890 PRIMER_INTERNAL_1_PENALTY=4.485568 PRIMER_LEFT_1_SEQUENCE=TTTCCCAAGATCACAGGTCA PRIMER_RIGHT_1_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA PRIMER_INTERNAL_1_SEQUENCE=TCAGGAGACTCATGTCTTTGAA PRIMER_LEFT_1=35,20 PRIMER_RIGHT_1=134,23 PRIMER_INTERNAL_1=67,22 PRIMER_LEFT_1_TM=59.059 PRIMER_RIGHT_1_TM=59.382 PRIMER_INTERNAL_1_TM=57.514 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=34.783 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=4.713782 PRIMER_LEFT_2_PENALTY=1.095892 PRIMER_RIGHT_2_PENALTY=3.617890 PRIMER_INTERNAL_2_PENALTY=4.485568 PRIMER_LEFT_2_SEQUENCE=TTCCCAAGATCACAGGTCAAG PRIMER_RIGHT_2_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA PRIMER_INTERNAL_2_SEQUENCE=TCAGGAGACTCATGTCTTTGAA PRIMER_LEFT_2=36,21 PRIMER_RIGHT_2=134,23 PRIMER_INTERNAL_2=67,22 PRIMER_LEFT_2_TM=60.096 PRIMER_RIGHT_2_TM=59.382 PRIMER_INTERNAL_2_TM=57.514 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=34.783 PRIMER_INTERNAL_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=4.976841 PRIMER_LEFT_3_PENALTY=1.358951 PRIMER_RIGHT_3_PENALTY=3.617890 PRIMER_INTERNAL_3_PENALTY=4.485568 PRIMER_LEFT_3_SEQUENCE=CCCAAGATCACAGGTCAAGA PRIMER_RIGHT_3_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA PRIMER_INTERNAL_3_SEQUENCE=TCAGGAGACTCATGTCTTTGAA PRIMER_LEFT_3=38,20 PRIMER_RIGHT_3=134,23 PRIMER_INTERNAL_3=67,22 PRIMER_LEFT_3_TM=58.641 PRIMER_RIGHT_3_TM=59.382 PRIMER_INTERNAL_3_TM=57.514 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=34.783 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=4.976841 PRIMER_LEFT_4_PENALTY=1.358951 PRIMER_RIGHT_4_PENALTY=3.617890 PRIMER_INTERNAL_4_PENALTY=4.485568 PRIMER_LEFT_4_SEQUENCE=TCCCAAGATCACAGGTCAAG PRIMER_RIGHT_4_SEQUENCE=CCCCTTTTCATTTCTATTGTTCA PRIMER_INTERNAL_4_SEQUENCE=TCAGGAGACTCATGTCTTTGAA PRIMER_LEFT_4=37,20 PRIMER_RIGHT_4=134,23 PRIMER_INTERNAL_4=67,22 PRIMER_LEFT_4_TM=58.641 PRIMER_RIGHT_4_TM=59.382 PRIMER_INTERNAL_4_TM=57.514 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=34.783 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH1022 SEQUENCE_TEMPLATE=GCTTGCATGCTNCAGGTCGACTCTAGAGGATCCCCTTTTTAAAAAAATATTTTCCCATGATTAAACACTTCTAGTGAACTTATAAAGGCCACCACTTCTCTCTCGTTCCTGCTCCTACACCACCACCTNCCCCTCAACTTGTTTTCCTCCTCCTTCACCTCCTGAATCAGCCAGCATAGAGTTTAGTTTATATAAACTTATTCAACCAGGGAAAATTTGGCAGGGATGTCATACTGATTGTTCTCACCTGTGGTAAAGGATTTGTGGATTGAGTCCTCTACTTAGGTACCATTTAATGATTAACCCCAAAGTGGGCAAAGAACCAGAAACCTGGAAG SEQUENCE_INCLUDED_REGION=35,302 P3_COMMENT=CL is AAAGGCCACCACTTCTCTCT P3_COMMENT=CR is TCCAGGTTTCTGGTTCTTTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.257880 PRIMER_LEFT_0_PENALTY=0.185942 PRIMER_RIGHT_0_PENALTY=0.071937 PRIMER_INTERNAL_0_PENALTY=5.504493 PRIMER_LEFT_0_SEQUENCE=TTCCTCCTCCTTCACCTCCT PRIMER_RIGHT_0_SEQUENCE=TCAGTATGACATCCCTGCCA PRIMER_INTERNAL_0_SEQUENCE=AAACTTATTCAACCAGGGAAAATT PRIMER_LEFT_0=143,20 PRIMER_RIGHT_0=236,20 PRIMER_INTERNAL_0=193,24 PRIMER_LEFT_0_TM=60.186 PRIMER_RIGHT_0_TM=60.072 PRIMER_INTERNAL_0_TM=58.496 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=29.167 PRIMER_LEFT_0_SELF_ANY=1.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=10.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=94 PRIMER_PAIR_1_PENALTY=0.268841 PRIMER_LEFT_1_PENALTY=0.196903 PRIMER_RIGHT_1_PENALTY=0.071937 PRIMER_INTERNAL_1_PENALTY=5.504493 PRIMER_LEFT_1_SEQUENCE=CTTCACCTCCTGAATCAGCC PRIMER_RIGHT_1_SEQUENCE=TCAGTATGACATCCCTGCCA PRIMER_INTERNAL_1_SEQUENCE=AAACTTATTCAACCAGGGAAAATT PRIMER_LEFT_1=152,20 PRIMER_RIGHT_1=236,20 PRIMER_INTERNAL_1=193,24 PRIMER_LEFT_1_TM=59.803 PRIMER_RIGHT_1_TM=60.072 PRIMER_INTERNAL_1_TM=58.496 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=29.167 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=85 PRIMER_PAIR_2_PENALTY=0.268841 PRIMER_LEFT_2_PENALTY=0.196903 PRIMER_RIGHT_2_PENALTY=0.071937 PRIMER_INTERNAL_2_PENALTY=5.504493 PRIMER_LEFT_2_SEQUENCE=CCTTCACCTCCTGAATCAGC PRIMER_RIGHT_2_SEQUENCE=TCAGTATGACATCCCTGCCA PRIMER_INTERNAL_2_SEQUENCE=AAACTTATTCAACCAGGGAAAATT PRIMER_LEFT_2=151,20 PRIMER_RIGHT_2=236,20 PRIMER_INTERNAL_2=193,24 PRIMER_LEFT_2_TM=59.803 PRIMER_RIGHT_2_TM=60.072 PRIMER_INTERNAL_2_TM=58.496 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=29.167 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=86 PRIMER_PAIR_3_PENALTY=0.413534 PRIMER_LEFT_3_PENALTY=0.185942 PRIMER_RIGHT_3_PENALTY=0.227592 PRIMER_INTERNAL_3_PENALTY=5.552018 PRIMER_LEFT_3_SEQUENCE=TTCCTCCTCCTTCACCTCCT PRIMER_RIGHT_3_SEQUENCE=CCAAATTTTCCCTGGTTGAA PRIMER_INTERNAL_3_SEQUENCE=TCAGCCAGCATAGAGTTTAGTTT PRIMER_LEFT_3=143,20 PRIMER_RIGHT_3=219,20 PRIMER_INTERNAL_3=166,23 PRIMER_LEFT_3_TM=60.186 PRIMER_RIGHT_3_TM=59.772 PRIMER_INTERNAL_3_TM=57.448 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=1.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=77 PRIMER_PAIR_4_PENALTY=0.495312 PRIMER_LEFT_4_PENALTY=0.185942 PRIMER_RIGHT_4_PENALTY=0.309369 PRIMER_INTERNAL_4_PENALTY=5.552018 PRIMER_LEFT_4_SEQUENCE=TTCCTCCTCCTTCACCTCCT PRIMER_RIGHT_4_SEQUENCE=TGACATCCCTGCCAAATTTT PRIMER_INTERNAL_4_SEQUENCE=TCAGCCAGCATAGAGTTTAGTTT PRIMER_LEFT_4=143,20 PRIMER_RIGHT_4=230,20 PRIMER_INTERNAL_4=166,23 PRIMER_LEFT_4_TM=60.186 PRIMER_RIGHT_4_TM=60.309 PRIMER_INTERNAL_4_TM=57.448 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=1.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=7.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=88 = SEQUENCE_ID=MH1026 SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTAAGCACTGAATGAAAATTAATTGTTAAATTCTGAGAATTGAACCTTCTTGTATTTTAATTTTGTTCAAACGTATTTTTTTGTTTTTCTTTTTTTTGTAAAAAAAAGAAAAAGCACAAACTTTCTTCTGCCTGCAACATTTCAGTAGGGTNNAACATCCTTTCTTTGTATTTGAATTCACTTTTTTGATGCAAGAAGAAAGGGAAAGATCTTGATGCAGGAAAATGGCATTGGCTCACAGATGCTATCTGCCATGCTTTTGTGCAG SEQUENCE_INCLUDED_REGION=34,267 P3_COMMENT=CL is ATTCTGAGAATTGAACCTTCTTG P3_COMMENT=CR is ACAAAAGCATGGCAGATAGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.383098 PRIMER_LEFT_0_PENALTY=0.012060 PRIMER_RIGHT_0_PENALTY=0.371037 PRIMER_INTERNAL_0_PENALTY=3.367771 PRIMER_LEFT_0_SEQUENCE=TTCTGCCTGCAACATTTCAG PRIMER_RIGHT_0_SEQUENCE=CCTGCATCAAGATCTTTCCC PRIMER_INTERNAL_0_SEQUENCE=TTGAATTCACTTTTTTGATGCAA PRIMER_LEFT_0=159,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=205,23 PRIMER_LEFT_0_TM=59.988 PRIMER_RIGHT_0_TM=59.629 PRIMER_INTERNAL_0_TM=59.632 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=26.087 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=96 PRIMER_PAIR_1_PENALTY=0.383098 PRIMER_LEFT_1_PENALTY=0.012060 PRIMER_RIGHT_1_PENALTY=0.371037 PRIMER_INTERNAL_1_PENALTY=3.367771 PRIMER_LEFT_1_SEQUENCE=CTTCTGCCTGCAACATTTCA PRIMER_RIGHT_1_SEQUENCE=CCTGCATCAAGATCTTTCCC PRIMER_INTERNAL_1_SEQUENCE=TTGAATTCACTTTTTTGATGCAA PRIMER_LEFT_1=158,20 PRIMER_RIGHT_1=254,20 PRIMER_INTERNAL_1=205,23 PRIMER_LEFT_1_TM=59.988 PRIMER_RIGHT_1_TM=59.629 PRIMER_INTERNAL_1_TM=59.632 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=26.087 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=97 PRIMER_PAIR_2_PENALTY=0.925873 PRIMER_LEFT_2_PENALTY=0.554836 PRIMER_RIGHT_2_PENALTY=0.371037 PRIMER_INTERNAL_2_PENALTY=3.367771 PRIMER_LEFT_2_SEQUENCE=TCTGCCTGCAACATTTCAGT PRIMER_RIGHT_2_SEQUENCE=CCTGCATCAAGATCTTTCCC PRIMER_INTERNAL_2_SEQUENCE=TTGAATTCACTTTTTTGATGCAA PRIMER_LEFT_2=160,20 PRIMER_RIGHT_2=254,20 PRIMER_INTERNAL_2=205,23 PRIMER_LEFT_2_TM=59.445 PRIMER_RIGHT_2_TM=59.629 PRIMER_INTERNAL_2_TM=59.632 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=26.087 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=95 PRIMER_PAIR_3_PENALTY=1.203231 PRIMER_LEFT_3_PENALTY=0.012060 PRIMER_RIGHT_3_PENALTY=1.191170 PRIMER_INTERNAL_3_PENALTY=6.335368 PRIMER_LEFT_3_SEQUENCE=TTCTGCCTGCAACATTTCAG PRIMER_RIGHT_3_SEQUENCE=TCCCTTTCTTCTTGCATCAAA PRIMER_INTERNAL_3_SEQUENCE=CATCCTTTCTTTGTATTTGAATTCA PRIMER_LEFT_3=159,20 PRIMER_RIGHT_3=238,21 PRIMER_INTERNAL_3=189,25 PRIMER_LEFT_3_TM=59.988 PRIMER_RIGHT_3_TM=59.809 PRIMER_INTERNAL_3_TM=58.665 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=38.095 PRIMER_INTERNAL_3_GC_PERCENT=28.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=8.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=1.203231 PRIMER_LEFT_4_PENALTY=0.012060 PRIMER_RIGHT_4_PENALTY=1.191170 PRIMER_INTERNAL_4_PENALTY=6.335368 PRIMER_LEFT_4_SEQUENCE=TTCTGCCTGCAACATTTCAG PRIMER_RIGHT_4_SEQUENCE=TTCCCTTTCTTCTTGCATCAA PRIMER_INTERNAL_4_SEQUENCE=CATCCTTTCTTTGTATTTGAATTCA PRIMER_LEFT_4=159,20 PRIMER_RIGHT_4=239,21 PRIMER_INTERNAL_4=189,25 PRIMER_LEFT_4_TM=59.988 PRIMER_RIGHT_4_TM=59.809 PRIMER_INTERNAL_4_TM=58.665 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_INTERNAL_4_GC_PERCENT=28.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=8.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=81 = SEQUENCE_ID=MH1027 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCTCTTATTCCATTCTGCCTCTCTCAGGGTGTTCTCCTCTGCCAAAGTGGGGGGACCCTCTTCTGATGTTTACGCGAGTCTCAAGTCCCCAGGGATTCATCTCACTGTGATTTAGCCAGGGCCTTGTTGCTTCTTCGGGAATCAGATGGCTGGACCACGAAAGCCCAGCGCCTTTCCTATCCTGAAAGAAG SEQUENCE_INCLUDED_REGION=36,192 P3_COMMENT=CL is GGGACCCTCTTCTGATGTTT P3_COMMENT=CR is TTCTTTCAGGATAGGAAAGGC P3_COMMENT=CM is CGCGAGTCTCAAGTCCCC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.085332 PRIMER_LEFT_0_PENALTY=0.014419 PRIMER_RIGHT_0_PENALTY=0.070913 PRIMER_INTERNAL_0_PENALTY=0.314094 PRIMER_LEFT_0_SEQUENCE=TGCCTCTCTCAGGGTGTTCT PRIMER_RIGHT_0_SEQUENCE=ATCCCTGGGGACTTGAGACT PRIMER_INTERNAL_0_SEQUENCE=GGGGACCCTCTTCTGATGTT PRIMER_LEFT_0=52,20 PRIMER_RIGHT_0=132,20 PRIMER_INTERNAL_0=87,20 PRIMER_LEFT_0_TM=59.986 PRIMER_RIGHT_0_TM=59.929 PRIMER_INTERNAL_0_TM=60.314 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=0.085332 PRIMER_LEFT_1_PENALTY=0.014419 PRIMER_RIGHT_1_PENALTY=0.070913 PRIMER_INTERNAL_1_PENALTY=0.314094 PRIMER_LEFT_1_SEQUENCE=CTGCCTCTCTCAGGGTGTTC PRIMER_RIGHT_1_SEQUENCE=ATCCCTGGGGACTTGAGACT PRIMER_INTERNAL_1_SEQUENCE=GGGGACCCTCTTCTGATGTT PRIMER_LEFT_1=51,20 PRIMER_RIGHT_1=132,20 PRIMER_INTERNAL_1=87,20 PRIMER_LEFT_1_TM=59.986 PRIMER_RIGHT_1_TM=59.929 PRIMER_INTERNAL_1_TM=60.314 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=0.085332 PRIMER_LEFT_2_PENALTY=0.014419 PRIMER_RIGHT_2_PENALTY=0.070913 PRIMER_INTERNAL_2_PENALTY=0.314094 PRIMER_LEFT_2_SEQUENCE=TCTGCCTCTCTCAGGGTGTT PRIMER_RIGHT_2_SEQUENCE=ATCCCTGGGGACTTGAGACT PRIMER_INTERNAL_2_SEQUENCE=GGGGACCCTCTTCTGATGTT PRIMER_LEFT_2=50,20 PRIMER_RIGHT_2=132,20 PRIMER_INTERNAL_2=87,20 PRIMER_LEFT_2_TM=59.986 PRIMER_RIGHT_2_TM=59.929 PRIMER_INTERNAL_2_TM=60.314 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.1000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=0.085332 PRIMER_LEFT_3_PENALTY=0.014419 PRIMER_RIGHT_3_PENALTY=0.070913 PRIMER_INTERNAL_3_PENALTY=0.314094 PRIMER_LEFT_3_SEQUENCE=TTCTGCCTCTCTCAGGGTGT PRIMER_RIGHT_3_SEQUENCE=ATCCCTGGGGACTTGAGACT PRIMER_INTERNAL_3_SEQUENCE=GGGGACCCTCTTCTGATGTT PRIMER_LEFT_3=49,20 PRIMER_RIGHT_3=132,20 PRIMER_INTERNAL_3=87,20 PRIMER_LEFT_3_TM=59.986 PRIMER_RIGHT_3_TM=59.929 PRIMER_INTERNAL_3_TM=60.314 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=84 PRIMER_PAIR_4_PENALTY=0.259542 PRIMER_LEFT_4_PENALTY=0.014419 PRIMER_RIGHT_4_PENALTY=0.245123 PRIMER_INTERNAL_4_PENALTY=0.314094 PRIMER_LEFT_4_SEQUENCE=CTCAGGGTGTTCTCCTCTGC PRIMER_RIGHT_4_SEQUENCE=AGATGAATCCCTGGGGACTT PRIMER_INTERNAL_4_SEQUENCE=GGGGACCCTCTTCTGATGTT PRIMER_LEFT_4=59,20 PRIMER_RIGHT_4=138,20 PRIMER_INTERNAL_4=87,20 PRIMER_LEFT_4_TM=59.986 PRIMER_RIGHT_4_TM=59.755 PRIMER_INTERNAL_4_TM=60.314 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH1033 SEQUENCE_TEMPLATE=ANTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGGAGCCAAAGCCACACCTGAACCCCTTTAAACCATACCTGGGGTGGTCAAGGAGTCTGCATCAGAATGTGGGAAGCAGAGACTTGAGGCAGCCCTGGGAAGTAAACCCCAAGATCTTCAGCGCACCCTAGGCTCTCTTTTGCAGGATTTCCTTCCCTCAGATCATGACACTCTGGGCCTATGACGGGAGGNGCTACGCAGTAATCTNCAAAGTACCTTTGAGGTTATTCTCCCATTGTCCTAATTAGTAG SEQUENCE_INCLUDED_REGION=33,252 P3_COMMENT=CL is CCTGAACCCCTTTAAACCAT P3_COMMENT=CR is ATGGGAGAATAACCTCAAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.142150 PRIMER_LEFT_0_PENALTY=0.119809 PRIMER_RIGHT_0_PENALTY=0.022341 PRIMER_INTERNAL_0_PENALTY=0.102380 PRIMER_LEFT_0_SEQUENCE=GGTGGTCAAGGAGTCTGCAT PRIMER_RIGHT_0_SEQUENCE=TAGGGTGCGCTGAAGATCTT PRIMER_INTERNAL_0_SEQUENCE=AAGCAGAGACTTGAGGCAGC PRIMER_LEFT_0=76,20 PRIMER_RIGHT_0=163,20 PRIMER_INTERNAL_0=107,20 PRIMER_LEFT_0_TM=60.120 PRIMER_RIGHT_0_TM=59.978 PRIMER_INTERNAL_0_TM=59.898 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=8.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 PRIMER_PAIR_1_PENALTY=0.223326 PRIMER_LEFT_1_PENALTY=0.200985 PRIMER_RIGHT_1_PENALTY=0.022341 PRIMER_INTERNAL_1_PENALTY=1.312895 PRIMER_LEFT_1_SEQUENCE=TCTGCATCAGAATGTGGGAA PRIMER_RIGHT_1_SEQUENCE=TAGGGTGCGCTGAAGATCTT PRIMER_INTERNAL_1_SEQUENCE=AGCCCTGGGAAGTAAACCC PRIMER_LEFT_1=89,20 PRIMER_RIGHT_1=163,20 PRIMER_INTERNAL_1=124,19 PRIMER_LEFT_1_TM=60.201 PRIMER_RIGHT_1_TM=59.978 PRIMER_INTERNAL_1_TM=60.313 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=8.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=0.278771 PRIMER_LEFT_2_PENALTY=0.176390 PRIMER_RIGHT_2_PENALTY=0.102380 PRIMER_INTERNAL_2_PENALTY=0.119809 PRIMER_LEFT_2_SEQUENCE=CCACACCTGAACCCCTTTAA PRIMER_RIGHT_2_SEQUENCE=GCTGCCTCAAGTCTCTGCTT PRIMER_INTERNAL_2_SEQUENCE=GGTGGTCAAGGAGTCTGCAT PRIMER_LEFT_2=45,20 PRIMER_RIGHT_2=126,20 PRIMER_INTERNAL_2=76,20 PRIMER_LEFT_2_TM=59.824 PRIMER_RIGHT_2_TM=59.898 PRIMER_INTERNAL_2_TM=60.120 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=82 PRIMER_PAIR_3_PENALTY=0.385340 PRIMER_LEFT_3_PENALTY=0.362999 PRIMER_RIGHT_3_PENALTY=0.022341 PRIMER_INTERNAL_3_PENALTY=1.312895 PRIMER_LEFT_3_SEQUENCE=GTCTGCATCAGAATGTGGGA PRIMER_RIGHT_3_SEQUENCE=TAGGGTGCGCTGAAGATCTT PRIMER_INTERNAL_3_SEQUENCE=AGCCCTGGGAAGTAAACCC PRIMER_LEFT_3=88,20 PRIMER_RIGHT_3=163,20 PRIMER_INTERNAL_3=124,19 PRIMER_LEFT_3_TM=59.637 PRIMER_RIGHT_3_TM=59.978 PRIMER_INTERNAL_3_TM=60.313 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=57.895 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=8.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=0.424452 PRIMER_LEFT_4_PENALTY=0.402111 PRIMER_RIGHT_4_PENALTY=0.022341 PRIMER_INTERNAL_4_PENALTY=0.102380 PRIMER_LEFT_4_SEQUENCE=TGGTCAAGGAGTCTGCATCA PRIMER_RIGHT_4_SEQUENCE=TAGGGTGCGCTGAAGATCTT PRIMER_INTERNAL_4_SEQUENCE=AAGCAGAGACTTGAGGCAGC PRIMER_LEFT_4=78,20 PRIMER_RIGHT_4=163,20 PRIMER_INTERNAL_4=107,20 PRIMER_LEFT_4_TM=60.402 PRIMER_RIGHT_4_TM=59.978 PRIMER_INTERNAL_4_TM=59.898 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=8.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH1034 SEQUENCE_TEMPLATE=GCATGNCTNCAGGTCGACTCTAGAGGATCCCCTTCCTGGTTGGCAAGACTTCATAAATTTTGCCATTATTTGAAGATAACCCACACCTTGGTGTCCAGGGTTTTCACAGGTATTAGTGGTCAGTCACATAGGCATATAGTACCTGTATGACTTCTATTCCAGCCACCGCAAACTTCTCCTCTCCCTGCTGGCTCCTGAGCCAAAAACAGGCATTTACCATAAATCACTTTGTTAGGATGAACTTATCTGGCCAAACTGATACGGCATGACCCACAGCCTCAGGTATATAAAACACTCTCATCAGGCAGAATGTTTCAGGGTCTCAGAGGTTAGTCAGGGGCTAGTCAAGGACCAATCCTGAAGGCAGGCC SEQUENCE_INCLUDED_REGION=32,338 P3_COMMENT=CL is TTTGCCATTATTTGAAGATAACC P3_COMMENT=CR is GGATTGGTCCTTGACTAGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.155909 PRIMER_LEFT_0_PENALTY=0.044832 PRIMER_RIGHT_0_PENALTY=0.111077 PRIMER_INTERNAL_0_PENALTY=0.532607 PRIMER_LEFT_0_SEQUENCE=ATCTGGCCAAACTGATACGG PRIMER_RIGHT_0_SEQUENCE=ACCCTGAAACATTCTGCCTG PRIMER_INTERNAL_0_SEQUENCE=TGACCCACAGCCTCAGGTAT PRIMER_LEFT_0=244,20 PRIMER_RIGHT_0=320,20 PRIMER_INTERNAL_0=266,20 PRIMER_LEFT_0_TM=59.955 PRIMER_RIGHT_0_TM=60.111 PRIMER_INTERNAL_0_TM=60.533 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=8.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=0.409870 PRIMER_LEFT_1_PENALTY=0.399325 PRIMER_RIGHT_1_PENALTY=0.010545 PRIMER_INTERNAL_1_PENALTY=3.690231 PRIMER_LEFT_1_SEQUENCE=TGTCCAGGGTTTTCACAGGT PRIMER_RIGHT_1_SEQUENCE=GAGAGGAGAAGTTTGCGGTG PRIMER_INTERNAL_1_SEQUENCE=TTAGTGGTCAGTCACATAGGCA PRIMER_LEFT_1=91,20 PRIMER_RIGHT_1=182,20 PRIMER_INTERNAL_1=112,22 PRIMER_LEFT_1_TM=60.399 PRIMER_RIGHT_1_TM=59.989 PRIMER_INTERNAL_1_TM=58.310 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=9.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=92 PRIMER_PAIR_2_PENALTY=0.409870 PRIMER_LEFT_2_PENALTY=0.399325 PRIMER_RIGHT_2_PENALTY=0.010545 PRIMER_INTERNAL_2_PENALTY=3.690231 PRIMER_LEFT_2_SEQUENCE=GTGTCCAGGGTTTTCACAGG PRIMER_RIGHT_2_SEQUENCE=GAGAGGAGAAGTTTGCGGTG PRIMER_INTERNAL_2_SEQUENCE=TTAGTGGTCAGTCACATAGGCA PRIMER_LEFT_2=90,20 PRIMER_RIGHT_2=182,20 PRIMER_INTERNAL_2=112,22 PRIMER_LEFT_2_TM=60.399 PRIMER_RIGHT_2_TM=59.989 PRIMER_INTERNAL_2_TM=58.310 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=93 PRIMER_PAIR_3_PENALTY=0.409870 PRIMER_LEFT_3_PENALTY=0.399325 PRIMER_RIGHT_3_PENALTY=0.010545 PRIMER_INTERNAL_3_PENALTY=3.690231 PRIMER_LEFT_3_SEQUENCE=GGTGTCCAGGGTTTTCACAG PRIMER_RIGHT_3_SEQUENCE=GAGAGGAGAAGTTTGCGGTG PRIMER_INTERNAL_3_SEQUENCE=TTAGTGGTCAGTCACATAGGCA PRIMER_LEFT_3=89,20 PRIMER_RIGHT_3=182,20 PRIMER_INTERNAL_3=112,22 PRIMER_LEFT_3_TM=60.399 PRIMER_RIGHT_3_TM=59.989 PRIMER_INTERNAL_3_TM=58.310 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=45.455 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=9.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=0.429134 PRIMER_LEFT_4_PENALTY=0.248900 PRIMER_RIGHT_4_PENALTY=0.180233 PRIMER_INTERNAL_4_PENALTY=3.485357 PRIMER_LEFT_4_SEQUENCE=CTGAGCCAAAAACAGGCATT PRIMER_RIGHT_4_SEQUENCE=GGGTCATGCCGTATCAGTTT PRIMER_INTERNAL_4_SEQUENCE=TGTTAGGATGAACTTATCTGGCC PRIMER_LEFT_4=194,20 PRIMER_RIGHT_4=271,20 PRIMER_INTERNAL_4=229,23 PRIMER_LEFT_4_TM=60.249 PRIMER_RIGHT_4_TM=59.820 PRIMER_INTERNAL_4_TM=59.515 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=78 = SEQUENCE_ID=MH1036 SEQUENCE_TEMPLATE=AGACAGGAGGAAGGCAGCAGAATGGTCTGACAAAGATGTCTGCAGGCAGGAGGGTCTGCAGAATTCATACCTAATTCCCTCAGTTTTCTTGGTGAACCAGGGGGTGGTGACTTGTGCTAGGCACCCAAACTAGAGACCAATCACTCCTTTTCACAAACATTTTATAAAGAATAGTCTTGTTCTCTTCTAATGAAGAAAGAGTTTTCTCTGCTCTTTTAGTTAGGCTGCTGAGCAC SEQUENCE_INCLUDED_REGION=0,235 P3_COMMENT=CL is CAGCAGAATGGTCTGACAAA P3_COMMENT=CR is TGCTCAGCAGCCTAACTAAA P3_COMMENT=CM is ATGTCTGCAGGCAGGAGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.103949 PRIMER_LEFT_0_PENALTY=0.008461 PRIMER_RIGHT_0_PENALTY=0.095488 PRIMER_INTERNAL_0_PENALTY=0.575355 PRIMER_LEFT_0_SEQUENCE=GACAAAGATGTCTGCAGGCA PRIMER_RIGHT_0_SEQUENCE=TGCCTAGCACAAGTCACCAC PRIMER_INTERNAL_0_SEQUENCE=GTTTTCTTGGTGAACCAGGG PRIMER_LEFT_0=28,20 PRIMER_RIGHT_0=122,20 PRIMER_INTERNAL_0=82,20 PRIMER_LEFT_0_TM=59.992 PRIMER_RIGHT_0_TM=59.905 PRIMER_INTERNAL_0_TM=59.425 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=8.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=95 PRIMER_PAIR_1_PENALTY=0.103949 PRIMER_LEFT_1_PENALTY=0.008461 PRIMER_RIGHT_1_PENALTY=0.095488 PRIMER_INTERNAL_1_PENALTY=0.575355 PRIMER_LEFT_1_SEQUENCE=GACAAAGATGTCTGCAGGCA PRIMER_RIGHT_1_SEQUENCE=GTGCCTAGCACAAGTCACCA PRIMER_INTERNAL_1_SEQUENCE=GTTTTCTTGGTGAACCAGGG PRIMER_LEFT_1=28,20 PRIMER_RIGHT_1=123,20 PRIMER_INTERNAL_1=82,20 PRIMER_LEFT_1_TM=59.992 PRIMER_RIGHT_1_TM=59.905 PRIMER_INTERNAL_1_TM=59.425 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=8.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=0.103949 PRIMER_LEFT_2_PENALTY=0.008461 PRIMER_RIGHT_2_PENALTY=0.095488 PRIMER_INTERNAL_2_PENALTY=0.196903 PRIMER_LEFT_2_SEQUENCE=TGACAAAGATGTCTGCAGGC PRIMER_RIGHT_2_SEQUENCE=TGCCTAGCACAAGTCACCAC PRIMER_INTERNAL_2_SEQUENCE=AGGAGGGTCTGCAGAATTCA PRIMER_LEFT_2=27,20 PRIMER_RIGHT_2=122,20 PRIMER_INTERNAL_2=47,20 PRIMER_LEFT_2_TM=59.992 PRIMER_RIGHT_2_TM=59.905 PRIMER_INTERNAL_2_TM=59.803 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=0.103949 PRIMER_LEFT_3_PENALTY=0.008461 PRIMER_RIGHT_3_PENALTY=0.095488 PRIMER_INTERNAL_3_PENALTY=0.196903 PRIMER_LEFT_3_SEQUENCE=TGACAAAGATGTCTGCAGGC PRIMER_RIGHT_3_SEQUENCE=GTGCCTAGCACAAGTCACCA PRIMER_INTERNAL_3_SEQUENCE=AGGAGGGTCTGCAGAATTCA PRIMER_LEFT_3=27,20 PRIMER_RIGHT_3=123,20 PRIMER_INTERNAL_3=47,20 PRIMER_LEFT_3_TM=59.992 PRIMER_RIGHT_3_TM=59.905 PRIMER_INTERNAL_3_TM=59.803 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=0.292391 PRIMER_LEFT_4_PENALTY=0.196903 PRIMER_RIGHT_4_PENALTY=0.095488 PRIMER_INTERNAL_4_PENALTY=0.575355 PRIMER_LEFT_4_SEQUENCE=AGGAGGGTCTGCAGAATTCA PRIMER_RIGHT_4_SEQUENCE=TGCCTAGCACAAGTCACCAC PRIMER_INTERNAL_4_SEQUENCE=GTTTTCTTGGTGAACCAGGG PRIMER_LEFT_4=47,20 PRIMER_RIGHT_4=122,20 PRIMER_INTERNAL_4=82,20 PRIMER_LEFT_4_TM=59.803 PRIMER_RIGHT_4_TM=59.905 PRIMER_INTERNAL_4_TM=59.425 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH1040 SEQUENCE_TEMPLATE=CNNCNNCCTCCAGGTCGACTCTAGAGGATCCCCCTTCACAAAAAGATAAGCAAAGAAGGAATGATATTAAAACTCTTTTACCTGGAGAAGAGGGTTGCATTCCNTTAGACTGTATAATGTGGCTTCAGACAGTGATGCAGGGGGAAGTTATATGCCTTCCTGGGAACTCATGATTAGAGAAGTCAGTAGAAGAAAACAAAGTTGAGAGTGAGGCCAAGACACGACCAGAGAAAAATGAGAGAGTTGTTCATTGAATGAGTTAAGTCAAAGAAAATGGAAGGAAAATTATGAGGTCTGTGGGCTATCTAATGCTCCTATTATGAAGTGTGGTTGAGTTGGAGG SEQUENCE_INCLUDED_REGION=33,309 P3_COMMENT=CL is CACAAAAAGATAAGCAAAGAAGG P3_COMMENT=CR is TCCAACTCAACCACACTTCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.531943 PRIMER_LEFT_0_PENALTY=0.077340 PRIMER_RIGHT_0_PENALTY=0.454603 PRIMER_INTERNAL_0_PENALTY=3.081536 PRIMER_LEFT_0_SEQUENCE=TGCAGGGGGAAGTTATATGC PRIMER_RIGHT_0_SEQUENCE=TGTCTTGGCCTCACTCTCAA PRIMER_INTERNAL_0_SEQUENCE=TCCTGGGAACTCATGATTAGAGA PRIMER_LEFT_0=135,20 PRIMER_RIGHT_0=220,20 PRIMER_INTERNAL_0=157,23 PRIMER_LEFT_0_TM=59.923 PRIMER_RIGHT_0_TM=59.545 PRIMER_INTERNAL_0_TM=60.082 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.4000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=86 PRIMER_PAIR_1_PENALTY=0.788943 PRIMER_LEFT_1_PENALTY=0.195358 PRIMER_RIGHT_1_PENALTY=0.593585 PRIMER_INTERNAL_1_PENALTY=0.422979 PRIMER_LEFT_1_SEQUENCE=TGGAGAAGAGGGTTGCATTC PRIMER_RIGHT_1_SEQUENCE=TGAGTTCCCAGGAAGGCATA PRIMER_INTERNAL_1_SEQUENCE=GCTTCAGACAGTGATGCAGG PRIMER_LEFT_1=82,20 PRIMER_RIGHT_1=169,20 PRIMER_INTERNAL_1=121,20 PRIMER_LEFT_1_TM=60.195 PRIMER_RIGHT_1_TM=60.594 PRIMER_INTERNAL_1_TM=59.577 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=7.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=88 PRIMER_PAIR_2_PENALTY=0.793466 PRIMER_LEFT_2_PENALTY=0.716126 PRIMER_RIGHT_2_PENALTY=0.077340 PRIMER_INTERNAL_2_PENALTY=4.642980 PRIMER_LEFT_2_SEQUENCE=TACCTGGAGAAGAGGGTTGC PRIMER_RIGHT_2_SEQUENCE=GCATATAACTTCCCCCTGCA PRIMER_INTERNAL_2_SEQUENCE=ATAATGTGGCTTCAGACAGTGA PRIMER_LEFT_2=78,20 PRIMER_RIGHT_2=154,20 PRIMER_INTERNAL_2=113,22 PRIMER_LEFT_2_TM=59.284 PRIMER_RIGHT_2_TM=59.923 PRIMER_INTERNAL_2_TM=57.357 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=0.877581 PRIMER_LEFT_3_PENALTY=0.422979 PRIMER_RIGHT_3_PENALTY=0.454603 PRIMER_INTERNAL_3_PENALTY=0.593585 PRIMER_LEFT_3_SEQUENCE=GCTTCAGACAGTGATGCAGG PRIMER_RIGHT_3_SEQUENCE=TGTCTTGGCCTCACTCTCAA PRIMER_INTERNAL_3_SEQUENCE=TATGCCTTCCTGGGAACTCA PRIMER_LEFT_3=121,20 PRIMER_RIGHT_3=220,20 PRIMER_INTERNAL_3=150,20 PRIMER_LEFT_3_TM=59.577 PRIMER_RIGHT_3_TM=59.545 PRIMER_INTERNAL_3_TM=60.594 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=0.904432 PRIMER_LEFT_4_PENALTY=0.449829 PRIMER_RIGHT_4_PENALTY=0.454603 PRIMER_INTERNAL_4_PENALTY=0.593585 PRIMER_LEFT_4_SEQUENCE=AGTGATGCAGGGGGAAGTTA PRIMER_RIGHT_4_SEQUENCE=TGTCTTGGCCTCACTCTCAA PRIMER_INTERNAL_4_SEQUENCE=TATGCCTTCCTGGGAACTCA PRIMER_LEFT_4=130,20 PRIMER_RIGHT_4=220,20 PRIMER_INTERNAL_4=150,20 PRIMER_LEFT_4_TM=59.550 PRIMER_RIGHT_4_TM=59.545 PRIMER_INTERNAL_4_TM=60.594 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=5.7000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH1041 SEQUENCE_TEMPLATE=GCTNCNNGCCTCCNGGTCGACTCTAGAGGATCCCTGTCCCAAATCAATCTTACCAGAACAATCTCAAAANANATGTTTTCTCTTCTGATATACATGAACATGGCCCAAAAAACATAAAAAAAAGAAGAAAATACCTCTTACTGGTTGATGAGGACCCCACATACATCCTGAGAATAAGGCACACGTAATGGATTGTGAAACCTCACATGAGAAATAGGTCTGGATGATGTTGTTTGGGCTTCCAAATCAAACAGTAG SEQUENCE_INCLUDED_REGION=34,223 P3_COMMENT=CL is CCAAATCAATCTTACCAGAACA P3_COMMENT=CR is GAAGCCCAAACAACATCATC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.236882 PRIMER_LEFT_0_PENALTY=0.152999 PRIMER_RIGHT_0_PENALTY=0.083883 PRIMER_INTERNAL_0_PENALTY=3.004943 PRIMER_LEFT_0_SEQUENCE=GGCACACGTAATGGATTGTG PRIMER_RIGHT_0_SEQUENCE=GTTTGATTTGGAAGCCCAAA PRIMER_INTERNAL_0_SEQUENCE=CCTCACATGAGAAATAGGTCTGG PRIMER_LEFT_0=177,20 PRIMER_RIGHT_0=251,20 PRIMER_INTERNAL_0=200,23 PRIMER_LEFT_0_TM=59.847 PRIMER_RIGHT_0_TM=59.916 PRIMER_INTERNAL_0_TM=60.005 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=47.826 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=0.441344 PRIMER_LEFT_1_PENALTY=0.237978 PRIMER_RIGHT_1_PENALTY=0.203367 PRIMER_INTERNAL_1_PENALTY=0.152999 PRIMER_LEFT_1_SEQUENCE=TGATGAGGACCCCACATACA PRIMER_RIGHT_1_SEQUENCE=AAGCCCAAACAACATCATCC PRIMER_INTERNAL_1_SEQUENCE=GGCACACGTAATGGATTGTG PRIMER_LEFT_1=145,20 PRIMER_RIGHT_1=240,20 PRIMER_INTERNAL_1=177,20 PRIMER_LEFT_1_TM=59.762 PRIMER_RIGHT_1_TM=59.797 PRIMER_INTERNAL_1_TM=59.847 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=5.6000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=0.441344 PRIMER_LEFT_2_PENALTY=0.237978 PRIMER_RIGHT_2_PENALTY=0.203367 PRIMER_INTERNAL_2_PENALTY=0.152999 PRIMER_LEFT_2_SEQUENCE=TGATGAGGACCCCACATACA PRIMER_RIGHT_2_SEQUENCE=GGAAGCCCAAACAACATCAT PRIMER_INTERNAL_2_SEQUENCE=GGCACACGTAATGGATTGTG PRIMER_LEFT_2=145,20 PRIMER_RIGHT_2=242,20 PRIMER_INTERNAL_2=177,20 PRIMER_LEFT_2_TM=59.762 PRIMER_RIGHT_2_TM=59.797 PRIMER_INTERNAL_2_TM=59.847 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=5.6000 PRIMER_RIGHT_2_END_STABILITY=6.5000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=98 PRIMER_PAIR_3_PENALTY=0.452013 PRIMER_LEFT_3_PENALTY=0.214036 PRIMER_RIGHT_3_PENALTY=0.237978 PRIMER_INTERNAL_3_PENALTY=8.781094 PRIMER_LEFT_3_SEQUENCE=TACATGAACATGGCCCAAAA PRIMER_RIGHT_3_SEQUENCE=TGTATGTGGGGTCCTCATCA PRIMER_INTERNAL_3_SEQUENCE=AAAAAAGAAGAAAATACCTCTTACTGG PRIMER_LEFT_3=90,20 PRIMER_RIGHT_3=164,20 PRIMER_INTERNAL_3=117,27 PRIMER_LEFT_3_TM=59.786 PRIMER_RIGHT_3_TM=59.762 PRIMER_INTERNAL_3_TM=58.219 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=29.630 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=0.548580 PRIMER_LEFT_4_PENALTY=0.345214 PRIMER_RIGHT_4_PENALTY=0.203367 PRIMER_INTERNAL_4_PENALTY=0.152999 PRIMER_LEFT_4_SEQUENCE=AGGACCCCACATACATCCTG PRIMER_RIGHT_4_SEQUENCE=AAGCCCAAACAACATCATCC PRIMER_INTERNAL_4_SEQUENCE=GGCACACGTAATGGATTGTG PRIMER_LEFT_4=150,20 PRIMER_RIGHT_4=240,20 PRIMER_INTERNAL_4=177,20 PRIMER_LEFT_4_TM=59.655 PRIMER_RIGHT_4_TM=59.797 PRIMER_INTERNAL_4_TM=59.847 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH1044 SEQUENCE_TEMPLATE=NNTNCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTAGCCTGCTTCATGGCAAGGAAAACAAAGTTATCTCAGCCTCATTTAGTACATTTCCTGCCCCACTCCTNNNTAAGAATCAATTTCTCCAATGAGCCCTGGTTCCTTTTACTAGGAAATGGTATTTAGAAACCACAGTCTGGGCTTTAGGGGTAGGCATTGGCCTTTTTAGTAACAGAACTAGTATTTTGGTATTTNAGGTAAATAAAACATATTTCAAATTCAAATTTAAAA SEQUENCE_INCLUDED_REGION=35,234 P3_COMMENT=CL is CATGGCAAGGAAAACAAAGT P3_COMMENT=CR is GCCCAGACTGTGGTTTCTAA P3_COMMENT=CM is ATCTCAGCCTCATTTAGTACATTTC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.019181 PRIMER_LEFT_0_PENALTY=0.013607 PRIMER_RIGHT_0_PENALTY=0.005574 PRIMER_INTERNAL_0_PENALTY=4.640064 PRIMER_LEFT_0_SEQUENCE=TCAATTTCTCCAATGAGCCC PRIMER_RIGHT_0_SEQUENCE=ACCCCTAAAGCCCAGACTGT PRIMER_INTERNAL_0_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT PRIMER_LEFT_0=114,20 PRIMER_RIGHT_0=188,20 PRIMER_INTERNAL_0=134,24 PRIMER_LEFT_0_TM=60.014 PRIMER_RIGHT_0_TM=59.994 PRIMER_INTERNAL_0_TM=59.360 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=37.500 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=0.058511 PRIMER_LEFT_1_PENALTY=0.013607 PRIMER_RIGHT_1_PENALTY=0.044904 PRIMER_INTERNAL_1_PENALTY=4.640064 PRIMER_LEFT_1_SEQUENCE=TCAATTTCTCCAATGAGCCC PRIMER_RIGHT_1_SEQUENCE=CCTACCCCTAAAGCCCAGAC PRIMER_INTERNAL_1_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT PRIMER_LEFT_1=114,20 PRIMER_RIGHT_1=191,20 PRIMER_INTERNAL_1=134,24 PRIMER_LEFT_1_TM=60.014 PRIMER_RIGHT_1_TM=59.955 PRIMER_INTERNAL_1_TM=59.360 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=8.00 PRIMER_LEFT_1_END_STABILITY=10.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=0.822899 PRIMER_LEFT_2_PENALTY=0.013607 PRIMER_RIGHT_2_PENALTY=0.809292 PRIMER_INTERNAL_2_PENALTY=4.640064 PRIMER_LEFT_2_SEQUENCE=TCAATTTCTCCAATGAGCCC PRIMER_RIGHT_2_SEQUENCE=TACCCCTAAAGCCCAGACTG PRIMER_INTERNAL_2_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT PRIMER_LEFT_2=114,20 PRIMER_RIGHT_2=189,20 PRIMER_INTERNAL_2=134,24 PRIMER_LEFT_2_TM=60.014 PRIMER_RIGHT_2_TM=59.191 PRIMER_INTERNAL_2_TM=59.360 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=10.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=8.00 PRIMER_LEFT_2_END_STABILITY=10.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=76 PRIMER_PAIR_3_PENALTY=0.825095 PRIMER_LEFT_3_PENALTY=0.013607 PRIMER_RIGHT_3_PENALTY=0.811488 PRIMER_INTERNAL_3_PENALTY=4.640064 PRIMER_LEFT_3_SEQUENCE=TCAATTTCTCCAATGAGCCC PRIMER_RIGHT_3_SEQUENCE=CAATGCCTACCCCTAAAGCC PRIMER_INTERNAL_3_SEQUENCE=TGGTTCCTTTTACTAGGAAATGGT PRIMER_LEFT_3=114,20 PRIMER_RIGHT_3=196,20 PRIMER_INTERNAL_3=134,24 PRIMER_LEFT_3_TM=60.014 PRIMER_RIGHT_3_TM=60.811 PRIMER_INTERNAL_3_TM=59.360 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=37.500 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=10.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=8.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=0.825095 PRIMER_LEFT_4_PENALTY=0.013607 PRIMER_RIGHT_4_PENALTY=0.811488 PRIMER_INTERNAL_4_PENALTY=4.529250 PRIMER_LEFT_4_SEQUENCE=TCAATTTCTCCAATGAGCCC PRIMER_RIGHT_4_SEQUENCE=CCAATGCCTACCCCTAAAGC PRIMER_INTERNAL_4_SEQUENCE=TGGTATTTAGAAACCACAGTCTGG PRIMER_LEFT_4=114,20 PRIMER_RIGHT_4=197,20 PRIMER_INTERNAL_4=154,24 PRIMER_LEFT_4_TM=60.014 PRIMER_RIGHT_4_TM=60.811 PRIMER_INTERNAL_4_TM=59.471 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=10.9000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=84 = SEQUENCE_ID=MH1045 SEQUENCE_TEMPLATE=CTGTAGGAAAACCTATTTNNTNATTTCTCGAAATATTTCCTCCCACCCATTTTTNCTGTACTGTCTTCCGAGATNTCCTATTATAGGTTGCACATTCCTAAAATCCTGAAAATCTGAAATCCAAAATGCTCTAAAATCTGAAACTTTTTGAATGCCAACATGATGCCACTAGTGGAAAGCTGAAAGTTTCACACCTGACCTCATGTGATGAGTCGCAGTAGAAACACAGGCACGTAACACAAAGTTTATTCAGCATTTCCAGAGGGAAAAAGATCTTCCCAG SEQUENCE_INCLUDED_REGION=0,282 P3_COMMENT=CL is TATTTCCTCCCACCCATTTT P3_COMMENT=CR is TGGGAAGATCTTTTTCCCTC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.529843 PRIMER_LEFT_0_PENALTY=0.044752 PRIMER_RIGHT_0_PENALTY=0.485091 PRIMER_INTERNAL_0_PENALTY=1.013478 PRIMER_LEFT_0_SEQUENCE=ATGCCAACATGATGCCACTA PRIMER_RIGHT_0_SEQUENCE=GTGCCTGTGTTTCTACTGCG PRIMER_INTERNAL_0_SEQUENCE=CACACCTGACCTCATGTGATG PRIMER_LEFT_0=151,20 PRIMER_RIGHT_0=232,20 PRIMER_INTERNAL_0=189,21 PRIMER_LEFT_0_TM=59.955 PRIMER_RIGHT_0_TM=59.515 PRIMER_INTERNAL_0_TM=60.013 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=5.7000 PRIMER_RIGHT_0_END_STABILITY=10.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=0.529843 PRIMER_LEFT_1_PENALTY=0.044752 PRIMER_RIGHT_1_PENALTY=0.485091 PRIMER_INTERNAL_1_PENALTY=1.013478 PRIMER_LEFT_1_SEQUENCE=ATGCCAACATGATGCCACTA PRIMER_RIGHT_1_SEQUENCE=CGTGCCTGTGTTTCTACTGC PRIMER_INTERNAL_1_SEQUENCE=CACACCTGACCTCATGTGATG PRIMER_LEFT_1=151,20 PRIMER_RIGHT_1=233,20 PRIMER_INTERNAL_1=189,21 PRIMER_LEFT_1_TM=59.955 PRIMER_RIGHT_1_TM=59.515 PRIMER_INTERNAL_1_TM=60.013 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=8.00 PRIMER_LEFT_1_END_STABILITY=5.7000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=0.641193 PRIMER_LEFT_2_PENALTY=0.044752 PRIMER_RIGHT_2_PENALTY=0.596442 PRIMER_INTERNAL_2_PENALTY=1.013478 PRIMER_LEFT_2_SEQUENCE=ATGCCAACATGATGCCACTA PRIMER_RIGHT_2_SEQUENCE=TGCCTGTGTTTCTACTGCGA PRIMER_INTERNAL_2_SEQUENCE=CACACCTGACCTCATGTGATG PRIMER_LEFT_2=151,20 PRIMER_RIGHT_2=231,20 PRIMER_INTERNAL_2=189,21 PRIMER_LEFT_2_TM=59.955 PRIMER_RIGHT_2_TM=60.596 PRIMER_INTERNAL_2_TM=60.013 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=8.00 PRIMER_LEFT_2_END_STABILITY=5.7000 PRIMER_RIGHT_2_END_STABILITY=10.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=0.960711 PRIMER_LEFT_3_PENALTY=0.475619 PRIMER_RIGHT_3_PENALTY=0.485091 PRIMER_INTERNAL_3_PENALTY=1.013478 PRIMER_LEFT_3_SEQUENCE=TTTGAATGCCAACATGATGC PRIMER_RIGHT_3_SEQUENCE=GTGCCTGTGTTTCTACTGCG PRIMER_INTERNAL_3_SEQUENCE=CACACCTGACCTCATGTGATG PRIMER_LEFT_3=146,20 PRIMER_RIGHT_3=232,20 PRIMER_INTERNAL_3=189,21 PRIMER_LEFT_3_TM=60.476 PRIMER_RIGHT_3_TM=59.515 PRIMER_INTERNAL_3_TM=60.013 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=8.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=10.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=87 PRIMER_PAIR_4_PENALTY=0.960711 PRIMER_LEFT_4_PENALTY=0.475619 PRIMER_RIGHT_4_PENALTY=0.485091 PRIMER_INTERNAL_4_PENALTY=1.013478 PRIMER_LEFT_4_SEQUENCE=TTTGAATGCCAACATGATGC PRIMER_RIGHT_4_SEQUENCE=CGTGCCTGTGTTTCTACTGC PRIMER_INTERNAL_4_SEQUENCE=CACACCTGACCTCATGTGATG PRIMER_LEFT_4=146,20 PRIMER_RIGHT_4=233,20 PRIMER_INTERNAL_4=189,21 PRIMER_LEFT_4_TM=60.476 PRIMER_RIGHT_4_TM=59.515 PRIMER_INTERNAL_4_TM=60.013 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=8.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=88 = SEQUENCE_ID=MH1046 SEQUENCE_TEMPLATE=GGAGCCATTCTCCTCGGTCTCGCTGGCCTGCCATGGAGAACACAAGGGCATNNGGTGGCCAGGCCNATGCAGCCAGGCTCCAGGCATCCCCAGGATCTCAGCCCCTCCAAGGGTACCCAGAATATTGAGGCACAGAGAGAAGCGACTGGCCTGAATACATACCTGGCTCCGCATCCCTGTGCCTCTNAGGACCCTAGACTCCCCTGATTCAGCCTT SEQUENCE_INCLUDED_REGION=0,216 P3_COMMENT=CL is GCCATTCTCCTCGGTCTC P3_COMMENT=CR is AATCAGGGGAGTCTAGGGTC P3_COMMENT=CM is GCCTGCCATGGAGAACAC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.238489 PRIMER_LEFT_0_PENALTY=0.022341 PRIMER_RIGHT_0_PENALTY=0.216148 PRIMER_INTERNAL_0_PENALTY=2.185516 PRIMER_LEFT_0_SEQUENCE=AGAAGCGACTGGCCTGAATA PRIMER_RIGHT_0_SEQUENCE=AGGCTGAATCAGGGGAGTCT PRIMER_INTERNAL_0_SEQUENCE=CATACCTGGCTCCGCATC PRIMER_LEFT_0=137,20 PRIMER_RIGHT_0=214,20 PRIMER_INTERNAL_0=157,18 PRIMER_LEFT_0_TM=59.978 PRIMER_RIGHT_0_TM=60.216 PRIMER_INTERNAL_0_TM=60.186 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 PRIMER_PAIR_1_PENALTY=0.358199 PRIMER_LEFT_1_PENALTY=0.292425 PRIMER_RIGHT_1_PENALTY=0.065775 PRIMER_INTERNAL_1_PENALTY=0.443661 PRIMER_LEFT_1_SEQUENCE=GAGGCACAGAGAGAAGCGAC PRIMER_RIGHT_1_SEQUENCE=TCAGGGGAGTCTAGGGTCCT PRIMER_INTERNAL_1_SEQUENCE=GAATACATACCTGGCTCCGC PRIMER_LEFT_1=126,20 PRIMER_RIGHT_1=206,20 PRIMER_INTERNAL_1=152,20 PRIMER_LEFT_1_TM=60.292 PRIMER_RIGHT_1_TM=60.066 PRIMER_INTERNAL_1_TM=59.556 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=81 PRIMER_PAIR_2_PENALTY=0.373365 PRIMER_LEFT_2_PENALTY=0.157217 PRIMER_RIGHT_2_PENALTY=0.216148 PRIMER_INTERNAL_2_PENALTY=3.006580 PRIMER_LEFT_2_SEQUENCE=GAAGCGACTGGCCTGAATAC PRIMER_RIGHT_2_SEQUENCE=AGGCTGAATCAGGGGAGTCT PRIMER_INTERNAL_2_SEQUENCE=ATACCTGGCTCCGCATCC PRIMER_LEFT_2=138,20 PRIMER_RIGHT_2=214,20 PRIMER_INTERNAL_2=158,18 PRIMER_LEFT_2_TM=59.843 PRIMER_RIGHT_2_TM=60.216 PRIMER_INTERNAL_2_TM=61.007 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=5.6000 PRIMER_RIGHT_2_END_STABILITY=6.1000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=5.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=0.508572 PRIMER_LEFT_3_PENALTY=0.292425 PRIMER_RIGHT_3_PENALTY=0.216148 PRIMER_INTERNAL_3_PENALTY=0.443661 PRIMER_LEFT_3_SEQUENCE=GAGGCACAGAGAGAAGCGAC PRIMER_RIGHT_3_SEQUENCE=AGGCTGAATCAGGGGAGTCT PRIMER_INTERNAL_3_SEQUENCE=GAATACATACCTGGCTCCGC PRIMER_LEFT_3=126,20 PRIMER_RIGHT_3=214,20 PRIMER_INTERNAL_3=152,20 PRIMER_LEFT_3_TM=60.292 PRIMER_RIGHT_3_TM=60.216 PRIMER_INTERNAL_3_TM=59.556 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=9.6000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=89 PRIMER_PAIR_4_PENALTY=0.618163 PRIMER_LEFT_4_PENALTY=0.022341 PRIMER_RIGHT_4_PENALTY=0.595822 PRIMER_INTERNAL_4_PENALTY=2.185516 PRIMER_LEFT_4_SEQUENCE=AGAAGCGACTGGCCTGAATA PRIMER_RIGHT_4_SEQUENCE=AAGGCTGAATCAGGGGAGTC PRIMER_INTERNAL_4_SEQUENCE=CATACCTGGCTCCGCATC PRIMER_LEFT_4=137,20 PRIMER_RIGHT_4=215,20 PRIMER_INTERNAL_4=157,18 PRIMER_LEFT_4_TM=59.978 PRIMER_RIGHT_4_TM=60.596 PRIMER_INTERNAL_4_TM=60.186 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=5.9000 PRIMER_RIGHT_4_END_STABILITY=6.1000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=6.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH1049 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAATCAACATGGGATGGCTCAGCTGAAACCAACATGGGGTGGCTTCAGGCCATCCCAGGCATTCAGAGGGTTCGTTTAATGACATTCACTGAGGCCCTGTCTATGTCAGGCCCTTGGTGTTGAAGACGCAATCATGAACAAAAATGAAAATACAATGTGATGGTCTCCTGAGTGTCTGAATGCGCCAGGTGGCTAAGTGCTGGGGCTCTGGGGTCAGGCTGCCTGGGTCACATCCTGGCTCCAAACTGCTTTGCTATGGCTTCGGGCAGTTACCTCATCTGTAAAATGGG SEQUENCE_INCLUDED_REGION=36,293 P3_COMMENT=CL is GCATTCAGAGGGTTCGTTTA P3_COMMENT=CR is AGCCATAGCAAAGCAGTTTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.310062 PRIMER_LEFT_0_PENALTY=0.243800 PRIMER_RIGHT_0_PENALTY=0.066262 PRIMER_INTERNAL_0_PENALTY=1.018537 PRIMER_LEFT_0_SEQUENCE=AAATCAACATGGGATGGCTC PRIMER_RIGHT_0_SEQUENCE=GGGCCTCAGTGAATGTCATT PRIMER_INTERNAL_0_SEQUENCE=AGGCATTCAGAGGGTTCGTT PRIMER_LEFT_0=39,20 PRIMER_RIGHT_0=135,20 PRIMER_INTERNAL_0=95,20 PRIMER_LEFT_0_TM=59.756 PRIMER_RIGHT_0_TM=59.934 PRIMER_INTERNAL_0_TM=61.019 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=7.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=97 PRIMER_PAIR_1_PENALTY=0.310062 PRIMER_LEFT_1_PENALTY=0.243800 PRIMER_RIGHT_1_PENALTY=0.066262 PRIMER_INTERNAL_1_PENALTY=1.018537 PRIMER_LEFT_1_SEQUENCE=GAAATCAACATGGGATGGCT PRIMER_RIGHT_1_SEQUENCE=GGGCCTCAGTGAATGTCATT PRIMER_INTERNAL_1_SEQUENCE=AGGCATTCAGAGGGTTCGTT PRIMER_LEFT_1=38,20 PRIMER_RIGHT_1=135,20 PRIMER_INTERNAL_1=95,20 PRIMER_LEFT_1_TM=59.756 PRIMER_RIGHT_1_TM=59.934 PRIMER_INTERNAL_1_TM=61.019 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=98 PRIMER_PAIR_2_PENALTY=0.329116 PRIMER_LEFT_2_PENALTY=0.262854 PRIMER_RIGHT_2_PENALTY=0.066262 PRIMER_INTERNAL_2_PENALTY=1.018537 PRIMER_LEFT_2_SEQUENCE=GGCTCAGCTGAAACCAACAT PRIMER_RIGHT_2_SEQUENCE=GGGCCTCAGTGAATGTCATT PRIMER_INTERNAL_2_SEQUENCE=AGGCATTCAGAGGGTTCGTT PRIMER_LEFT_2=54,20 PRIMER_RIGHT_2=135,20 PRIMER_INTERNAL_2=95,20 PRIMER_LEFT_2_TM=60.263 PRIMER_RIGHT_2_TM=59.934 PRIMER_INTERNAL_2_TM=61.019 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=7.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=82 PRIMER_PAIR_3_PENALTY=0.329116 PRIMER_LEFT_3_PENALTY=0.262854 PRIMER_RIGHT_3_PENALTY=0.066262 PRIMER_INTERNAL_3_PENALTY=1.018537 PRIMER_LEFT_3_SEQUENCE=ATGGCTCAGCTGAAACCAAC PRIMER_RIGHT_3_SEQUENCE=GGGCCTCAGTGAATGTCATT PRIMER_INTERNAL_3_SEQUENCE=AGGCATTCAGAGGGTTCGTT PRIMER_LEFT_3=52,20 PRIMER_RIGHT_3=135,20 PRIMER_INTERNAL_3=95,20 PRIMER_LEFT_3_TM=60.263 PRIMER_RIGHT_3_TM=59.934 PRIMER_INTERNAL_3_TM=61.019 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=7.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=84 PRIMER_PAIR_4_PENALTY=0.363609 PRIMER_LEFT_4_PENALTY=0.243800 PRIMER_RIGHT_4_PENALTY=0.119809 PRIMER_INTERNAL_4_PENALTY=1.018537 PRIMER_LEFT_4_SEQUENCE=AAATCAACATGGGATGGCTC PRIMER_RIGHT_4_SEQUENCE=ACAGGGCCTCAGTGAATGTC PRIMER_INTERNAL_4_SEQUENCE=AGGCATTCAGAGGGTTCGTT PRIMER_LEFT_4=39,20 PRIMER_RIGHT_4=138,20 PRIMER_INTERNAL_4=95,20 PRIMER_LEFT_4_TM=59.756 PRIMER_RIGHT_4_TM=60.120 PRIMER_INTERNAL_4_TM=61.019 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=6.3000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH105 SEQUENCE_TEMPLATE=AGCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCTATAAAGGAGAGTATTTTAAAAGTGCCTCCCAGTAGGAAGAACAGTCACAAGGCACTGTTATATCAATTCAGTGTGACACAAGCCCTGATTATTTAATAGTATAACAGCAGTGAATCAGAGTTCTTTCATCTGACTTTGCTGACATTTCCAGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT SEQUENCE_INCLUDED_REGION=35,155 P3_COMMENT=CL is GTAGGAAGAACAGTCACAAGGC P3_COMMENT=CR is TGGAAATGTCAGCAAAGTCA P3_COMMENT=CM is TGTTATATCAATTCAGTGTGACACA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.816278 PRIMER_LEFT_0_PENALTY=0.690085 PRIMER_RIGHT_0_PENALTY=1.126193 PRIMER_INTERNAL_0_PENALTY=4.580191 PRIMER_LEFT_0_SEQUENCE=TTCAGTGTGACACAAGCCCT PRIMER_RIGHT_0_SEQUENCE=GCTGGAAATGTCAGCAAAGTC PRIMER_INTERNAL_0_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC PRIMER_LEFT_0=104,20 PRIMER_RIGHT_0=189,21 PRIMER_INTERNAL_0=144,24 PRIMER_LEFT_0_TM=59.310 PRIMER_RIGHT_0_TM=59.874 PRIMER_INTERNAL_0_TM=59.420 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=86 PRIMER_PAIR_1_PENALTY=2.064695 PRIMER_LEFT_1_PENALTY=0.938502 PRIMER_RIGHT_1_PENALTY=1.126193 PRIMER_INTERNAL_1_PENALTY=4.580191 PRIMER_LEFT_1_SEQUENCE=CAGTGTGACACAAGCCCTGA PRIMER_RIGHT_1_SEQUENCE=GCTGGAAATGTCAGCAAAGTC PRIMER_INTERNAL_1_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC PRIMER_LEFT_1=106,20 PRIMER_RIGHT_1=189,21 PRIMER_INTERNAL_1=144,24 PRIMER_LEFT_1_TM=60.939 PRIMER_RIGHT_1_TM=59.874 PRIMER_INTERNAL_1_TM=59.420 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_INTERNAL_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=84 PRIMER_PAIR_2_PENALTY=2.064695 PRIMER_LEFT_2_PENALTY=0.938502 PRIMER_RIGHT_2_PENALTY=1.126193 PRIMER_INTERNAL_2_PENALTY=4.580191 PRIMER_LEFT_2_SEQUENCE=TCAGTGTGACACAAGCCCTG PRIMER_RIGHT_2_SEQUENCE=GCTGGAAATGTCAGCAAAGTC PRIMER_INTERNAL_2_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC PRIMER_LEFT_2=105,20 PRIMER_RIGHT_2=189,21 PRIMER_INTERNAL_2=144,24 PRIMER_LEFT_2_TM=60.939 PRIMER_RIGHT_2_TM=59.874 PRIMER_INTERNAL_2_TM=59.420 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=85 PRIMER_PAIR_3_PENALTY=2.153786 PRIMER_LEFT_3_PENALTY=1.027592 PRIMER_RIGHT_3_PENALTY=1.126193 PRIMER_INTERNAL_3_PENALTY=4.580191 PRIMER_LEFT_3_SEQUENCE=AATTCAGTGTGACACAAGCCC PRIMER_RIGHT_3_SEQUENCE=GCTGGAAATGTCAGCAAAGTC PRIMER_INTERNAL_3_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC PRIMER_LEFT_3=102,21 PRIMER_RIGHT_3=189,21 PRIMER_INTERNAL_3=144,24 PRIMER_LEFT_3_TM=60.028 PRIMER_RIGHT_3_TM=59.874 PRIMER_INTERNAL_3_TM=59.420 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=88 PRIMER_PAIR_4_PENALTY=2.489066 PRIMER_LEFT_4_PENALTY=1.362873 PRIMER_RIGHT_4_PENALTY=1.126193 PRIMER_INTERNAL_4_PENALTY=4.580191 PRIMER_LEFT_4_SEQUENCE=AGTGTGACACAAGCCCTGATT PRIMER_RIGHT_4_SEQUENCE=GCTGGAAATGTCAGCAAAGTC PRIMER_INTERNAL_4_SEQUENCE=GCAGTGAATCAGAGTTCTTTCATC PRIMER_LEFT_4=107,21 PRIMER_RIGHT_4=189,21 PRIMER_INTERNAL_4=144,24 PRIMER_LEFT_4_TM=59.637 PRIMER_RIGHT_4_TM=59.874 PRIMER_INTERNAL_4_TM=59.420 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=83 = SEQUENCE_ID=MH1051 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGTGAACTCATATAATTTTCTTTCCAGCCTGTTACTATACATATATGCATGATGTCCATGCTCTCATCTAAAATCAGCCTCTGCTTGTGTACTAGATCAGGGTTGACAAAATGCAGCCTGTGGCCCAAATCCTGCATTTTGTTTTGTTTTTGTACAGCCCGTAAGCCAAGAATAGTTTTTACTTTTTTTTTTTTTTTTTTTTTTT SEQUENCE_INCLUDED_REGION=36,207 P3_COMMENT=CL is TATGCATGATGTCCATGCTC P3_COMMENT=CR is ACTATTCTTGGCTTACGGGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.531471 PRIMER_LEFT_0_PENALTY=0.308722 PRIMER_RIGHT_0_PENALTY=0.222749 PRIMER_INTERNAL_0_PENALTY=0.199246 PRIMER_LEFT_0_SEQUENCE=TCAGGGTTGACAAAATGCAG PRIMER_RIGHT_0_SEQUENCE=TATTCTTGGCTTACGGGCTG PRIMER_INTERNAL_0_SEQUENCE=CCCAAATCCTGCATTTTGTT PRIMER_LEFT_0=134,20 PRIMER_RIGHT_0=211,20 PRIMER_INTERNAL_0=161,20 PRIMER_LEFT_0_TM=59.691 PRIMER_RIGHT_0_TM=60.223 PRIMER_INTERNAL_0_TM=59.801 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 PRIMER_PAIR_1_PENALTY=0.579059 PRIMER_LEFT_1_PENALTY=0.308722 PRIMER_RIGHT_1_PENALTY=0.270338 PRIMER_INTERNAL_1_PENALTY=0.199246 PRIMER_LEFT_1_SEQUENCE=TCAGGGTTGACAAAATGCAG PRIMER_RIGHT_1_SEQUENCE=TCTTGGCTTACGGGCTGTAC PRIMER_INTERNAL_1_SEQUENCE=CCCAAATCCTGCATTTTGTT PRIMER_LEFT_1=134,20 PRIMER_RIGHT_1=208,20 PRIMER_INTERNAL_1=161,20 PRIMER_LEFT_1_TM=59.691 PRIMER_RIGHT_1_TM=60.270 PRIMER_INTERNAL_1_TM=59.801 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=5.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=1.017068 PRIMER_LEFT_2_PENALTY=0.817822 PRIMER_RIGHT_2_PENALTY=0.199246 PRIMER_INTERNAL_2_PENALTY=0.308722 PRIMER_LEFT_2_SEQUENCE=GCATGATGTCCATGCTCTCA PRIMER_RIGHT_2_SEQUENCE=AACAAAATGCAGGATTTGGG PRIMER_INTERNAL_2_SEQUENCE=TCAGGGTTGACAAAATGCAG PRIMER_LEFT_2=84,20 PRIMER_RIGHT_2=180,20 PRIMER_INTERNAL_2=134,20 PRIMER_LEFT_2_TM=60.818 PRIMER_RIGHT_2_TM=59.801 PRIMER_INTERNAL_2_TM=59.691 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=97 PRIMER_PAIR_3_PENALTY=1.017068 PRIMER_LEFT_3_PENALTY=0.817822 PRIMER_RIGHT_3_PENALTY=0.199246 PRIMER_INTERNAL_3_PENALTY=0.308722 PRIMER_LEFT_3_SEQUENCE=TGCATGATGTCCATGCTCTC PRIMER_RIGHT_3_SEQUENCE=AACAAAATGCAGGATTTGGG PRIMER_INTERNAL_3_SEQUENCE=TCAGGGTTGACAAAATGCAG PRIMER_LEFT_3=83,20 PRIMER_RIGHT_3=180,20 PRIMER_INTERNAL_3=134,20 PRIMER_LEFT_3_TM=60.818 PRIMER_RIGHT_3_TM=59.801 PRIMER_INTERNAL_3_TM=59.691 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=1.070958 PRIMER_LEFT_4_PENALTY=0.308722 PRIMER_RIGHT_4_PENALTY=0.762236 PRIMER_INTERNAL_4_PENALTY=0.199246 PRIMER_LEFT_4_SEQUENCE=TCAGGGTTGACAAAATGCAG PRIMER_RIGHT_4_SEQUENCE=TTCTTGGCTTACGGGCTGTA PRIMER_INTERNAL_4_SEQUENCE=CCCAAATCCTGCATTTTGTT PRIMER_LEFT_4=134,20 PRIMER_RIGHT_4=209,20 PRIMER_INTERNAL_4=161,20 PRIMER_LEFT_4_TM=59.691 PRIMER_RIGHT_4_TM=60.762 PRIMER_INTERNAL_4_TM=59.801 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=5.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH1055 SEQUENCE_TEMPLATE=TGCNTGCAGGTCGACTCTAGAGGATCCCCCTAACATTCCAGTGGAGGAACTAAAACTATTATCAAGTGACCAAATAAATGAGCAAGATTGTTTCAGAGAATGAAGTCTGTGGGGGAAAATAATAGGTTAGAGTGATCAGAGACAGAGAACTGGGTGGATAAAGGGGGCAGGAGAGACGTTGCTAGAAACCACTGTGTACGTTGAACATATTCTGACTCTGATCCTCCCAATGGAAGACCCTTATTCTTATTGCTTCTTCTTCTTTGTCTCCCCAGCCCCTGGCACAGAGTGAGCACTTAAAAACCACCTGTGTACATATGACTGACCTTTCCGTCACCTGCCTTCCCATTGGCCTCTTCCTGCCCCTTCATCCACCTTTGTGTTTGAATATTCATTCACATGGCTTGTTCTTC SEQUENCE_INCLUDED_REGION=29,384 P3_COMMENT=CL is AACATTCCAGTGGAGGAACT P3_COMMENT=CR is GAACAAGCCATGTGAATGAA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.253180 PRIMER_LEFT_0_PENALTY=0.096893 PRIMER_RIGHT_0_PENALTY=0.156287 PRIMER_INTERNAL_0_PENALTY=1.115240 PRIMER_LEFT_0_SEQUENCE=GAATGAAGTCTGTGGGGGAA PRIMER_RIGHT_0_SEQUENCE=CTAGCAACGTCTCTCCTGCC PRIMER_INTERNAL_0_SEQUENCE=CAGAGACAGAGAACTGGGTGG PRIMER_LEFT_0=97,20 PRIMER_RIGHT_0=184,20 PRIMER_INTERNAL_0=136,21 PRIMER_LEFT_0_TM=59.903 PRIMER_RIGHT_0_TM=60.156 PRIMER_INTERNAL_0_TM=59.885 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=57.143 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 PRIMER_PAIR_1_PENALTY=0.467584 PRIMER_LEFT_1_PENALTY=0.464275 PRIMER_RIGHT_1_PENALTY=0.003309 PRIMER_INTERNAL_1_PENALTY=3.012411 PRIMER_LEFT_1_SEQUENCE=ATAAAGGGGGCAGGAGAGAC PRIMER_RIGHT_1_SEQUENCE=TTCCATTGGGAGGATCAGAG PRIMER_INTERNAL_1_SEQUENCE=GAAACCACTGTGTACGTTGAACA PRIMER_LEFT_1=157,20 PRIMER_RIGHT_1=234,20 PRIMER_INTERNAL_1=184,23 PRIMER_LEFT_1_TM=59.536 PRIMER_RIGHT_1_TM=60.003 PRIMER_INTERNAL_1_TM=59.988 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=43.478 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.1000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=0.467584 PRIMER_LEFT_2_PENALTY=0.464275 PRIMER_RIGHT_2_PENALTY=0.003309 PRIMER_INTERNAL_2_PENALTY=3.012411 PRIMER_LEFT_2_SEQUENCE=ATAAAGGGGGCAGGAGAGAC PRIMER_RIGHT_2_SEQUENCE=CTTCCATTGGGAGGATCAGA PRIMER_INTERNAL_2_SEQUENCE=GAAACCACTGTGTACGTTGAACA PRIMER_LEFT_2=157,20 PRIMER_RIGHT_2=235,20 PRIMER_INTERNAL_2=184,23 PRIMER_LEFT_2_TM=59.536 PRIMER_RIGHT_2_TM=60.003 PRIMER_INTERNAL_2_TM=59.988 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.1000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=79 PRIMER_PAIR_3_PENALTY=0.467584 PRIMER_LEFT_3_PENALTY=0.464275 PRIMER_RIGHT_3_PENALTY=0.003309 PRIMER_INTERNAL_3_PENALTY=3.012411 PRIMER_LEFT_3_SEQUENCE=ATAAAGGGGGCAGGAGAGAC PRIMER_RIGHT_3_SEQUENCE=TCTTCCATTGGGAGGATCAG PRIMER_INTERNAL_3_SEQUENCE=GAAACCACTGTGTACGTTGAACA PRIMER_LEFT_3=157,20 PRIMER_RIGHT_3=236,20 PRIMER_INTERNAL_3=184,23 PRIMER_LEFT_3_TM=59.536 PRIMER_RIGHT_3_TM=60.003 PRIMER_INTERNAL_3_TM=59.988 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.1000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=0.540641 PRIMER_LEFT_4_PENALTY=0.537332 PRIMER_RIGHT_4_PENALTY=0.003309 PRIMER_INTERNAL_4_PENALTY=3.012411 PRIMER_LEFT_4_SEQUENCE=GATAAAGGGGGCAGGAGAGA PRIMER_RIGHT_4_SEQUENCE=TTCCATTGGGAGGATCAGAG PRIMER_INTERNAL_4_SEQUENCE=GAAACCACTGTGTACGTTGAACA PRIMER_LEFT_4=156,20 PRIMER_RIGHT_4=234,20 PRIMER_INTERNAL_4=184,23 PRIMER_LEFT_4_TM=60.537 PRIMER_RIGHT_4_TM=60.003 PRIMER_INTERNAL_4_TM=59.988 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH1056 SEQUENCE_TEMPLATE=NNTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGTGTGTATTGAGGGAAGGGGAGATGTGTGCACACNTTCCAGAAAAATGGGGAGGTAGTTTAGAAAGTNTTTCTCTGTAACAGCACAG SEQUENCE_INCLUDED_REGION=36,89 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH1058 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCAATTTATAGGAAGTGAGGACAGGATAGCATGTTAAAGGGTATGGATGGCTTATAGGAGAGGGAAGGTTGAACTGAGTCCCCTGAAAGAGACCTGAGAAACATATCTACCAAATACAATGTTAACTTTGTTGGGATTCTGGTCCAAAGAAACGTACTAAAAAATATGGGGAGAAATAGAGGANTATGAATATGGCCTGAGTACTATAAGATATTAAAGAAAAATTATTAACTTGCTAGGTATGATAATGATATTTTGGTTATGTTTTTTAAAAAGTTGTTAAAAATACATAGTGAAGTACTTCAGGTGA SEQUENCE_INCLUDED_REGION=36,313 P3_COMMENT=CL is TAGGAAGTGAGGACAGGATAGC P3_COMMENT=CR is TCTTATAGTACTCAGGCCATATTCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.518143 PRIMER_LEFT_0_PENALTY=0.088170 PRIMER_RIGHT_0_PENALTY=0.429973 PRIMER_INTERNAL_0_PENALTY=6.837090 PRIMER_LEFT_0_SEQUENCE=GGTTGAACTGAGTCCCCTGA PRIMER_RIGHT_0_SEQUENCE=TCTTTGGACCAGAATCCCAA PRIMER_INTERNAL_0_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA PRIMER_LEFT_0=105,20 PRIMER_RIGHT_0=188,20 PRIMER_INTERNAL_0=127,26 PRIMER_LEFT_0_TM=60.088 PRIMER_RIGHT_0_TM=60.430 PRIMER_INTERNAL_0_TM=59.163 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=38.462 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=10.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=84 PRIMER_PAIR_1_PENALTY=0.518143 PRIMER_LEFT_1_PENALTY=0.088170 PRIMER_RIGHT_1_PENALTY=0.429973 PRIMER_INTERNAL_1_PENALTY=6.837090 PRIMER_LEFT_1_SEQUENCE=GGTTGAACTGAGTCCCCTGA PRIMER_RIGHT_1_SEQUENCE=TTCTTTGGACCAGAATCCCA PRIMER_INTERNAL_1_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA PRIMER_LEFT_1=105,20 PRIMER_RIGHT_1=189,20 PRIMER_INTERNAL_1=127,26 PRIMER_LEFT_1_TM=60.088 PRIMER_RIGHT_1_TM=60.430 PRIMER_INTERNAL_1_TM=59.163 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=38.462 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=85 PRIMER_PAIR_2_PENALTY=0.707220 PRIMER_LEFT_2_PENALTY=0.088170 PRIMER_RIGHT_2_PENALTY=0.619050 PRIMER_INTERNAL_2_PENALTY=6.837090 PRIMER_LEFT_2_SEQUENCE=GGTTGAACTGAGTCCCCTGA PRIMER_RIGHT_2_SEQUENCE=GGACCAGAATCCCAACAAAG PRIMER_INTERNAL_2_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA PRIMER_LEFT_2=105,20 PRIMER_RIGHT_2=183,20 PRIMER_INTERNAL_2=127,26 PRIMER_LEFT_2_TM=60.088 PRIMER_RIGHT_2_TM=59.381 PRIMER_INTERNAL_2_TM=59.163 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=38.462 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=7.3000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=79 PRIMER_PAIR_3_PENALTY=0.707220 PRIMER_LEFT_3_PENALTY=0.088170 PRIMER_RIGHT_3_PENALTY=0.619050 PRIMER_INTERNAL_3_PENALTY=6.837090 PRIMER_LEFT_3_SEQUENCE=GGTTGAACTGAGTCCCCTGA PRIMER_RIGHT_3_SEQUENCE=CTTTGGACCAGAATCCCAAC PRIMER_INTERNAL_3_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA PRIMER_LEFT_3=105,20 PRIMER_RIGHT_3=187,20 PRIMER_INTERNAL_3=127,26 PRIMER_LEFT_3_TM=60.088 PRIMER_RIGHT_3_TM=59.381 PRIMER_INTERNAL_3_TM=59.163 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=38.462 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=1.131882 PRIMER_LEFT_4_PENALTY=0.088170 PRIMER_RIGHT_4_PENALTY=1.043712 PRIMER_INTERNAL_4_PENALTY=6.837090 PRIMER_LEFT_4_SEQUENCE=GGTTGAACTGAGTCCCCTGA PRIMER_RIGHT_4_SEQUENCE=TTTCTTTGGACCAGAATCCC PRIMER_INTERNAL_4_SEQUENCE=GAGACCTGAGAAACATATCTACCAAA PRIMER_LEFT_4=105,20 PRIMER_RIGHT_4=190,20 PRIMER_INTERNAL_4=127,26 PRIMER_LEFT_4_TM=60.088 PRIMER_RIGHT_4_TM=58.956 PRIMER_INTERNAL_4_TM=59.163 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=38.462 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=9.3000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH1059 SEQUENCE_TEMPLATE=GGTGGATGCTNAGGTCGACTCTAGAGGATCCCCCTTATCTATTTACTTTTGCTACTTAAGTAAATAACTTATCACATCATAAGTGCATTGCNTTTGANGGTTTCAGTTGGTTGGCTTAGCACATGGTAAACACCAATTNGGCATTCTCTCTTGTNNAGTNGCCATTTGCAAAATGNATTATTTCAGATTTGCTGAAATACTGAATTTGTAAGTTTACACTCTGCTTATTCATATTTNTAAATAACTCTTTTGCCATTNGGTCCCAAACCCATGTGCTGATGTGTCTGCNGGGTCTCATTTCTTCCCCTGTATTAGTATCTGA SEQUENCE_INCLUDED_REGION=33,289 P3_COMMENT=CL is TTTCAGTTGGTTGGCTTAGC P3_COMMENT=CR is ACAGGGGAAGAAATGAGACC P3_COMMENT=CM is TATTTCAGATTTGCTGAAATACTGA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=8.757764 PRIMER_LEFT_0_PENALTY=6.805269 PRIMER_RIGHT_0_PENALTY=1.952496 PRIMER_INTERNAL_0_PENALTY=9.929484 PRIMER_LEFT_0_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT PRIMER_RIGHT_0_SEQUENCE=CATCAGCACATGGGTTTGG PRIMER_INTERNAL_0_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT PRIMER_LEFT_0=181,24 PRIMER_RIGHT_0=280,19 PRIMER_INTERNAL_0=205,27 PRIMER_LEFT_0_TM=57.195 PRIMER_RIGHT_0_TM=60.952 PRIMER_INTERNAL_0_TM=57.071 PRIMER_LEFT_0_GC_PERCENT=29.167 PRIMER_RIGHT_0_GC_PERCENT=52.632 PRIMER_INTERNAL_0_GC_PERCENT=29.630 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=7.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=9.486970 PRIMER_LEFT_1_PENALTY=6.805269 PRIMER_RIGHT_1_PENALTY=2.681702 PRIMER_INTERNAL_1_PENALTY=9.929484 PRIMER_LEFT_1_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT PRIMER_RIGHT_1_SEQUENCE=AGCACATGGGTTTGGGAC PRIMER_INTERNAL_1_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT PRIMER_LEFT_1=181,24 PRIMER_RIGHT_1=276,18 PRIMER_INTERNAL_1=205,27 PRIMER_LEFT_1_TM=57.195 PRIMER_RIGHT_1_TM=59.318 PRIMER_INTERNAL_1_TM=57.071 PRIMER_LEFT_1_GC_PERCENT=29.167 PRIMER_RIGHT_1_GC_PERCENT=55.556 PRIMER_INTERNAL_1_GC_PERCENT=29.630 PRIMER_LEFT_1_SELF_ANY=11.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=7.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=9.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=9.556564 PRIMER_LEFT_2_PENALTY=6.805269 PRIMER_RIGHT_2_PENALTY=2.751295 PRIMER_INTERNAL_2_PENALTY=9.929484 PRIMER_LEFT_2_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT PRIMER_RIGHT_2_SEQUENCE=ATCAGCACATGGGTTTGGG PRIMER_INTERNAL_2_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT PRIMER_LEFT_2=181,24 PRIMER_RIGHT_2=279,19 PRIMER_INTERNAL_2=205,27 PRIMER_LEFT_2_TM=57.195 PRIMER_RIGHT_2_TM=61.751 PRIMER_INTERNAL_2_TM=57.071 PRIMER_LEFT_2_GC_PERCENT=29.167 PRIMER_RIGHT_2_GC_PERCENT=52.632 PRIMER_INTERNAL_2_GC_PERCENT=29.630 PRIMER_LEFT_2_SELF_ANY=11.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=7.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=10.184613 PRIMER_LEFT_3_PENALTY=6.805269 PRIMER_RIGHT_3_PENALTY=3.379345 PRIMER_INTERNAL_3_PENALTY=9.929484 PRIMER_LEFT_3_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT PRIMER_RIGHT_3_SEQUENCE=CAGCACATGGGTTTGGGAC PRIMER_INTERNAL_3_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT PRIMER_LEFT_3=181,24 PRIMER_RIGHT_3=277,19 PRIMER_INTERNAL_3=205,27 PRIMER_LEFT_3_TM=57.195 PRIMER_RIGHT_3_TM=62.379 PRIMER_INTERNAL_3_TM=57.071 PRIMER_LEFT_3_GC_PERCENT=29.167 PRIMER_RIGHT_3_GC_PERCENT=57.895 PRIMER_INTERNAL_3_GC_PERCENT=29.630 PRIMER_LEFT_3_SELF_ANY=11.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=7.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=9.1000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=10.349774 PRIMER_LEFT_4_PENALTY=6.805269 PRIMER_RIGHT_4_PENALTY=3.544505 PRIMER_INTERNAL_4_PENALTY=9.929484 PRIMER_LEFT_4_SEQUENCE=TTCAGATTTGCTGAAATACTGAAT PRIMER_RIGHT_4_SEQUENCE=CAGCACATGGGTTTGGGA PRIMER_INTERNAL_4_SEQUENCE=TTGTAAGTTTACACTCTGCTTATTCAT PRIMER_LEFT_4=181,24 PRIMER_RIGHT_4=277,18 PRIMER_INTERNAL_4=205,27 PRIMER_LEFT_4_TM=57.195 PRIMER_RIGHT_4_TM=61.545 PRIMER_INTERNAL_4_TM=57.071 PRIMER_LEFT_4_GC_PERCENT=29.167 PRIMER_RIGHT_4_GC_PERCENT=55.556 PRIMER_INTERNAL_4_GC_PERCENT=29.630 PRIMER_LEFT_4_SELF_ANY=11.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=7.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=97 = SEQUENCE_ID=MH1064 SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGCTGTCAGTTTGCCACAGTTCTCACCATTCCCTAATCTATACCGCCCCTGCTTCATTCTTCATTCACTACTTGTGAGCAGACATCTGAGATGACTCTGGATTTATGTTTTAATCTCTTTATTTAAAACACACACACATACACTGCACACACACAAGTCACCACAATCACAACCACAAAGAAGTGCTTGAGTATGCTATAGCATGACCTTTTCTTCACATCTCTGCTCAAATATCTCCTTATCAGAGAGGCCTTCTCTTACTACCTTCTAAAATAGGACCCACTGCCCCTCTATGGGTCTCATTTCTCTTCACAGTACTCCAACACTTGACCCATTACTGATTCACTTGTCTACTCT SEQUENCE_INCLUDED_REGION=37,358 P3_COMMENT=CL is TTCCCTAATCTATACCGCCC P3_COMMENT=CR is CAGTAATGGGTCAAGTGTTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.696726 PRIMER_LEFT_0_PENALTY=0.273747 PRIMER_RIGHT_0_PENALTY=2.422979 PRIMER_INTERNAL_0_PENALTY=0.048570 PRIMER_LEFT_0_SEQUENCE=GTTTGCCACAGTTCTCACCA PRIMER_RIGHT_0_SEQUENCE=TCATCTCAGATGTCTGCTCACA PRIMER_INTERNAL_0_SEQUENCE=AATCTATACCGCCCCTGCTT PRIMER_LEFT_0=46,20 PRIMER_RIGHT_0=131,22 PRIMER_INTERNAL_0=72,20 PRIMER_LEFT_0_TM=59.726 PRIMER_RIGHT_0_TM=59.577 PRIMER_INTERNAL_0_TM=59.951 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=9.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=86 PRIMER_PAIR_1_PENALTY=2.740914 PRIMER_LEFT_1_PENALTY=0.317935 PRIMER_RIGHT_1_PENALTY=2.422979 PRIMER_INTERNAL_1_PENALTY=0.048570 PRIMER_LEFT_1_SEQUENCE=TGCCACAGTTCTCACCATTC PRIMER_RIGHT_1_SEQUENCE=TCATCTCAGATGTCTGCTCACA PRIMER_INTERNAL_1_SEQUENCE=AATCTATACCGCCCCTGCTT PRIMER_LEFT_1=49,20 PRIMER_RIGHT_1=131,22 PRIMER_INTERNAL_1=72,20 PRIMER_LEFT_1_TM=59.682 PRIMER_RIGHT_1_TM=59.577 PRIMER_INTERNAL_1_TM=59.951 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=9.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=2.785968 PRIMER_LEFT_2_PENALTY=0.362989 PRIMER_RIGHT_2_PENALTY=2.422979 PRIMER_INTERNAL_2_PENALTY=0.048570 PRIMER_LEFT_2_SEQUENCE=CCACAGTTCTCACCATTCCC PRIMER_RIGHT_2_SEQUENCE=TCATCTCAGATGTCTGCTCACA PRIMER_INTERNAL_2_SEQUENCE=AATCTATACCGCCCCTGCTT PRIMER_LEFT_2=51,20 PRIMER_RIGHT_2=131,22 PRIMER_INTERNAL_2=72,20 PRIMER_LEFT_2_TM=60.363 PRIMER_RIGHT_2_TM=59.577 PRIMER_INTERNAL_2_TM=59.951 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=9.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=2.933091 PRIMER_LEFT_3_PENALTY=0.510112 PRIMER_RIGHT_3_PENALTY=2.422979 PRIMER_INTERNAL_3_PENALTY=0.048570 PRIMER_LEFT_3_SEQUENCE=TGCTGTCAGTTTGCCACAGT PRIMER_RIGHT_3_SEQUENCE=TCATCTCAGATGTCTGCTCACA PRIMER_INTERNAL_3_SEQUENCE=AATCTATACCGCCCCTGCTT PRIMER_LEFT_3=38,20 PRIMER_RIGHT_3=131,22 PRIMER_INTERNAL_3=72,20 PRIMER_LEFT_3_TM=60.510 PRIMER_RIGHT_3_TM=59.577 PRIMER_INTERNAL_3_TM=59.951 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.455 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=9.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.1000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=2.942891 PRIMER_LEFT_4_PENALTY=0.519913 PRIMER_RIGHT_4_PENALTY=2.422979 PRIMER_INTERNAL_4_PENALTY=0.048570 PRIMER_LEFT_4_SEQUENCE=GCCACAGTTCTCACCATTCC PRIMER_RIGHT_4_SEQUENCE=TCATCTCAGATGTCTGCTCACA PRIMER_INTERNAL_4_SEQUENCE=AATCTATACCGCCCCTGCTT PRIMER_LEFT_4=50,20 PRIMER_RIGHT_4=131,22 PRIMER_INTERNAL_4=72,20 PRIMER_LEFT_4_TM=60.520 PRIMER_RIGHT_4_TM=59.577 PRIMER_INTERNAL_4_TM=59.951 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.455 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=9.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH1065 SEQUENCE_TEMPLATE=ATGCCGAGATTTGTTTTAAAGTTGGAATAGGAAAATTCCTTCTCTAACATATTTTATACCCCCTGTGGATGACCCTATACCTTACTGTCTAAATNTTGACTTTCTGAGAGTNNGAGGNNGCACTGCTGTTAATGACAATTAGATTGGAGATAAAATCCAAGACTCTTACAGNCAAACTTGGACACATGTCACCCTACCTATGAAGTGGGAATTAATACTGACCTAAAGACGATGATAGTATCAAGAACGAGGTCACGGGTTTTAGAGCAACTAG SEQUENCE_INCLUDED_REGION=0,274 P3_COMMENT=CL is TTTATACCCCCTGTGGATGA P3_COMMENT=CR is CCTCGTTCTTGATACTATCATCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.794957 PRIMER_LEFT_0_PENALTY=0.765704 PRIMER_RIGHT_0_PENALTY=0.029253 PRIMER_INTERNAL_0_PENALTY=5.798652 PRIMER_LEFT_0_SEQUENCE=TGGACACATGTCACCCTACC PRIMER_RIGHT_0_SEQUENCE=TAAAACCCGTGACCTCGTTC PRIMER_INTERNAL_0_SEQUENCE=TGAAGTGGGAATTAATACTGACC PRIMER_LEFT_0=178,20 PRIMER_RIGHT_0=263,20 PRIMER_INTERNAL_0=200,23 PRIMER_LEFT_0_TM=59.234 PRIMER_RIGHT_0_TM=59.971 PRIMER_INTERNAL_0_TM=57.201 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=39.130 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=86 PRIMER_PAIR_1_PENALTY=0.876096 PRIMER_LEFT_1_PENALTY=0.765704 PRIMER_RIGHT_1_PENALTY=0.110392 PRIMER_INTERNAL_1_PENALTY=5.798652 PRIMER_LEFT_1_SEQUENCE=TGGACACATGTCACCCTACC PRIMER_RIGHT_1_SEQUENCE=TTGCTCTAAAACCCGTGACC PRIMER_INTERNAL_1_SEQUENCE=TGAAGTGGGAATTAATACTGACC PRIMER_LEFT_1=178,20 PRIMER_RIGHT_1=269,20 PRIMER_INTERNAL_1=200,23 PRIMER_LEFT_1_TM=59.234 PRIMER_RIGHT_1_TM=60.110 PRIMER_INTERNAL_1_TM=57.201 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=92 PRIMER_PAIR_2_PENALTY=1.031419 PRIMER_LEFT_2_PENALTY=0.765704 PRIMER_RIGHT_2_PENALTY=0.265715 PRIMER_INTERNAL_2_PENALTY=5.798652 PRIMER_LEFT_2_SEQUENCE=TGGACACATGTCACCCTACC PRIMER_RIGHT_2_SEQUENCE=TGCTCTAAAACCCGTGACCT PRIMER_INTERNAL_2_SEQUENCE=TGAAGTGGGAATTAATACTGACC PRIMER_LEFT_2=178,20 PRIMER_RIGHT_2=268,20 PRIMER_INTERNAL_2=200,23 PRIMER_LEFT_2_TM=59.234 PRIMER_RIGHT_2_TM=59.734 PRIMER_INTERNAL_2_TM=57.201 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=91 PRIMER_PAIR_3_PENALTY=1.037384 PRIMER_LEFT_3_PENALTY=0.765704 PRIMER_RIGHT_3_PENALTY=0.271680 PRIMER_INTERNAL_3_PENALTY=5.798652 PRIMER_LEFT_3_SEQUENCE=TGGACACATGTCACCCTACC PRIMER_RIGHT_3_SEQUENCE=CTCTAAAACCCGTGACCTCG PRIMER_INTERNAL_3_SEQUENCE=TGAAGTGGGAATTAATACTGACC PRIMER_LEFT_3=178,20 PRIMER_RIGHT_3=266,20 PRIMER_INTERNAL_3=200,23 PRIMER_LEFT_3_TM=59.234 PRIMER_RIGHT_3_TM=59.728 PRIMER_INTERNAL_3_TM=57.201 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=9.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=89 PRIMER_PAIR_4_PENALTY=1.173645 PRIMER_LEFT_4_PENALTY=0.765704 PRIMER_RIGHT_4_PENALTY=0.407941 PRIMER_INTERNAL_4_PENALTY=5.798652 PRIMER_LEFT_4_SEQUENCE=TGGACACATGTCACCCTACC PRIMER_RIGHT_4_SEQUENCE=TCTAAAACCCGTGACCTCGT PRIMER_INTERNAL_4_SEQUENCE=TGAAGTGGGAATTAATACTGACC PRIMER_LEFT_4=178,20 PRIMER_RIGHT_4=265,20 PRIMER_INTERNAL_4=200,23 PRIMER_LEFT_4_TM=59.234 PRIMER_RIGHT_4_TM=59.592 PRIMER_INTERNAL_4_TM=57.201 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=88 = SEQUENCE_ID=MH1066 SEQUENCE_TEMPLATE=NTNGCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTATTCAATTTCTTTACTGCCTTCAAAAATTAATTCAATCTGTTGAGCATGAATTACCTTATGATCCCCTTTTGTATTGTTCTATCAATGTGATCTGTATCTCATGATGTTCAATTTTTCCACTGCACACACAGNCCCAGTCTAAATAAATGCTGGCTGACTAACTTTCTGCTCACCTCTTGCTGCTCAGATCTTTCTTAAAGAAAGGTTTTGCCATTCAGTTCCAAGGAGCACTATTGGAG SEQUENCE_INCLUDED_REGION=35,242 P3_COMMENT=CL is TTCAATTTCTTTACTGCCTTCA P3_COMMENT=CR is GTGCTCCTTGGAACTGAATG P3_COMMENT=CM is AAATTAATTCAATCTGTTGAGCATG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.701124 PRIMER_LEFT_0_PENALTY=1.141878 PRIMER_RIGHT_0_PENALTY=0.559246 PRIMER_INTERNAL_0_PENALTY=2.010077 PRIMER_LEFT_0_SEQUENCE=TCAATTTTTCCACTGCACACA PRIMER_RIGHT_0_SEQUENCE=TCTGAGCAGCAAGAGGTGAG PRIMER_INTERNAL_0_SEQUENCE=CCCAGTCTAAATAAATGCTGGC PRIMER_LEFT_0=145,21 PRIMER_RIGHT_0=225,20 PRIMER_INTERNAL_0=170,22 PRIMER_LEFT_0_TM=60.142 PRIMER_RIGHT_0_TM=59.441 PRIMER_INTERNAL_0_TM=59.990 PRIMER_LEFT_0_GC_PERCENT=38.095 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=1.828134 PRIMER_LEFT_1_PENALTY=1.141878 PRIMER_RIGHT_1_PENALTY=0.686256 PRIMER_INTERNAL_1_PENALTY=2.010077 PRIMER_LEFT_1_SEQUENCE=TCAATTTTTCCACTGCACACA PRIMER_RIGHT_1_SEQUENCE=CAGCAAGAGGTGAGCAGAAA PRIMER_INTERNAL_1_SEQUENCE=CCCAGTCTAAATAAATGCTGGC PRIMER_LEFT_1=145,21 PRIMER_RIGHT_1=219,20 PRIMER_INTERNAL_1=170,22 PRIMER_LEFT_1_TM=60.142 PRIMER_RIGHT_1_TM=59.314 PRIMER_INTERNAL_1_TM=59.990 PRIMER_LEFT_1_GC_PERCENT=38.095 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=2.254461 PRIMER_LEFT_2_PENALTY=1.695215 PRIMER_RIGHT_2_PENALTY=0.559246 PRIMER_INTERNAL_2_PENALTY=2.010077 PRIMER_LEFT_2_SEQUENCE=TTTTTCCACTGCACACACAG PRIMER_RIGHT_2_SEQUENCE=TCTGAGCAGCAAGAGGTGAG PRIMER_INTERNAL_2_SEQUENCE=CCCAGTCTAAATAAATGCTGGC PRIMER_LEFT_2=149,20 PRIMER_RIGHT_2=225,20 PRIMER_INTERNAL_2=170,22 PRIMER_LEFT_2_TM=58.305 PRIMER_RIGHT_2_TM=59.441 PRIMER_INTERNAL_2_TM=59.990 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=2.364539 PRIMER_LEFT_3_PENALTY=0.686256 PRIMER_RIGHT_3_PENALTY=1.678283 PRIMER_INTERNAL_3_PENALTY=2.271131 PRIMER_LEFT_3_SEQUENCE=TTTCTGCTCACCTCTTGCTG PRIMER_RIGHT_3_SEQUENCE=CTCCAATAGTGCTCCTTGGAA PRIMER_INTERNAL_3_SEQUENCE=AAAGAAAGGTTTTGCCATTCA PRIMER_LEFT_3=200,20 PRIMER_RIGHT_3=276,21 PRIMER_INTERNAL_3=234,21 PRIMER_LEFT_3_TM=59.314 PRIMER_RIGHT_3_TM=59.322 PRIMER_INTERNAL_3_TM=58.729 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=33.333 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=77 PRIMER_PAIR_4_PENALTY=2.381854 PRIMER_LEFT_4_PENALTY=1.141878 PRIMER_RIGHT_4_PENALTY=1.239976 PRIMER_INTERNAL_4_PENALTY=2.010077 PRIMER_LEFT_4_SEQUENCE=TCAATTTTTCCACTGCACACA PRIMER_RIGHT_4_SEQUENCE=ATCTGAGCAGCAAGAGGTGAG PRIMER_INTERNAL_4_SEQUENCE=CCCAGTCTAAATAAATGCTGGC PRIMER_LEFT_4=145,21 PRIMER_RIGHT_4=226,21 PRIMER_INTERNAL_4=170,22 PRIMER_LEFT_4_TM=60.142 PRIMER_RIGHT_4_TM=59.760 PRIMER_INTERNAL_4_TM=59.990 PRIMER_LEFT_4_GC_PERCENT=38.095 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=45.455 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH107 SEQUENCE_TEMPLATE=GCATTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTCCTTCTNACCCTGAACCAGGAAACTCACCGGCAGGGAATCAAGATGGGAAGTAAAAAGAAAATATTCCCCTAAGTCAGGNTCAGATTCTATCATGCCTGGCCACCTGGAGAAGATAGTTGGGTTTAGGGNNCAGAAGAGAAGGGNTTAGNTGNNCAGANCACANCCTNATCANTGTACATCCNCAG SEQUENCE_INCLUDED_REGION=36,188 P3_COMMENT=CL is ACCCTGAACCAGGAAACTCA P3_COMMENT=CR is ACTATCTTCTCCAGGTGGCC P3_COMMENT=CM is CGGCAGGGAATCAAGATG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.527440 PRIMER_LEFT_0_PENALTY=0.450843 PRIMER_RIGHT_0_PENALTY=0.076597 PRIMER_INTERNAL_0_PENALTY=0.521793 PRIMER_LEFT_0_SEQUENCE=CCTGAACCAGGAAACTCACC PRIMER_RIGHT_0_SEQUENCE=GTGGCCAGGCATGATAGAAT PRIMER_INTERNAL_0_SEQUENCE=CAGGGAATCAAGATGGGAAG PRIMER_LEFT_0=47,20 PRIMER_RIGHT_0=141,20 PRIMER_INTERNAL_0=69,20 PRIMER_LEFT_0_TM=59.549 PRIMER_RIGHT_0_TM=59.923 PRIMER_INTERNAL_0_TM=59.478 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=95 PRIMER_PAIR_1_PENALTY=0.527440 PRIMER_LEFT_1_PENALTY=0.450843 PRIMER_RIGHT_1_PENALTY=0.076597 PRIMER_INTERNAL_1_PENALTY=0.521793 PRIMER_LEFT_1_SEQUENCE=CCCTGAACCAGGAAACTCAC PRIMER_RIGHT_1_SEQUENCE=GTGGCCAGGCATGATAGAAT PRIMER_INTERNAL_1_SEQUENCE=CAGGGAATCAAGATGGGAAG PRIMER_LEFT_1=46,20 PRIMER_RIGHT_1=141,20 PRIMER_INTERNAL_1=69,20 PRIMER_LEFT_1_TM=59.549 PRIMER_RIGHT_1_TM=59.923 PRIMER_INTERNAL_1_TM=59.478 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=0.527440 PRIMER_LEFT_2_PENALTY=0.450843 PRIMER_RIGHT_2_PENALTY=0.076597 PRIMER_INTERNAL_2_PENALTY=0.521793 PRIMER_LEFT_2_SEQUENCE=ACCCTGAACCAGGAAACTCA PRIMER_RIGHT_2_SEQUENCE=GTGGCCAGGCATGATAGAAT PRIMER_INTERNAL_2_SEQUENCE=CAGGGAATCAAGATGGGAAG PRIMER_LEFT_2=45,20 PRIMER_RIGHT_2=141,20 PRIMER_INTERNAL_2=69,20 PRIMER_LEFT_2_TM=59.549 PRIMER_RIGHT_2_TM=59.923 PRIMER_INTERNAL_2_TM=59.478 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=97 PRIMER_PAIR_3_PENALTY=0.731663 PRIMER_LEFT_3_PENALTY=0.521793 PRIMER_RIGHT_3_PENALTY=0.209870 PRIMER_INTERNAL_3_PENALTY=5.617642 PRIMER_LEFT_3_SEQUENCE=CAGGGAATCAAGATGGGAAG PRIMER_RIGHT_3_SEQUENCE=TATCTTCTCCAGGTGGCCAG PRIMER_INTERNAL_3_SEQUENCE=AAAGAAAATATTCCCCTAAGTCAGG PRIMER_LEFT_3=69,20 PRIMER_RIGHT_3=153,20 PRIMER_INTERNAL_3=92,25 PRIMER_LEFT_3_TM=59.478 PRIMER_RIGHT_3_TM=60.210 PRIMER_INTERNAL_3_TM=59.382 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=36.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=85 PRIMER_PAIR_4_PENALTY=0.731663 PRIMER_LEFT_4_PENALTY=0.521793 PRIMER_RIGHT_4_PENALTY=0.209870 PRIMER_INTERNAL_4_PENALTY=5.617642 PRIMER_LEFT_4_SEQUENCE=CAGGGAATCAAGATGGGAAG PRIMER_RIGHT_4_SEQUENCE=CTATCTTCTCCAGGTGGCCA PRIMER_INTERNAL_4_SEQUENCE=AAAGAAAATATTCCCCTAAGTCAGG PRIMER_LEFT_4=69,20 PRIMER_RIGHT_4=154,20 PRIMER_INTERNAL_4=92,25 PRIMER_LEFT_4_TM=59.478 PRIMER_RIGHT_4_TM=60.210 PRIMER_INTERNAL_4_TM=59.382 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=36.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=11.2000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH1070 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGCTATATAGCCAAGTCGGTATTTATANNNTGTTTCCACCCTTGATAATTTCATGCCAGTACTCATCAGAATGGAACACTGACTTGGCTCTGTCTGTCCTTATTCTCATCTCCATCCCCTCCTTTCCAACACACTGCAATTCTAGATTGAAGACTGCACATTTATTTATTTATTTATTTTTTTGAGACGGA SEQUENCE_INCLUDED_REGION=36,193 P3_COMMENT=CL is CTTGCTATATAGCCAAGTCGG P3_COMMENT=CR is ATTGCAGTGTGTTGGAAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.697841 PRIMER_LEFT_0_PENALTY=1.570745 PRIMER_RIGHT_0_PENALTY=0.127097 PRIMER_INTERNAL_0_PENALTY=1.032397 PRIMER_LEFT_0_SEQUENCE=CCCTTGATAATTTCATGCCA PRIMER_RIGHT_0_SEQUENCE=TGCAGTGTGTTGGAAAGGAG PRIMER_INTERNAL_0_SEQUENCE=TGACTTGGCTCTGTCTGTCC PRIMER_LEFT_0=76,20 PRIMER_RIGHT_0=175,20 PRIMER_INTERNAL_0=117,20 PRIMER_LEFT_0_TM=58.429 PRIMER_RIGHT_0_TM=59.873 PRIMER_INTERNAL_0_TM=58.968 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=1.707283 PRIMER_LEFT_1_PENALTY=1.580186 PRIMER_RIGHT_1_PENALTY=0.127097 PRIMER_INTERNAL_1_PENALTY=1.032397 PRIMER_LEFT_1_SEQUENCE=CCCTTGATAATTTCATGCCAG PRIMER_RIGHT_1_SEQUENCE=TGCAGTGTGTTGGAAAGGAG PRIMER_INTERNAL_1_SEQUENCE=TGACTTGGCTCTGTCTGTCC PRIMER_LEFT_1=76,21 PRIMER_RIGHT_1=175,20 PRIMER_INTERNAL_1=117,20 PRIMER_LEFT_1_TM=59.420 PRIMER_RIGHT_1_TM=59.873 PRIMER_INTERNAL_1_TM=58.968 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=1.982967 PRIMER_LEFT_2_PENALTY=1.570745 PRIMER_RIGHT_2_PENALTY=0.412222 PRIMER_INTERNAL_2_PENALTY=1.032397 PRIMER_LEFT_2_SEQUENCE=CCCTTGATAATTTCATGCCA PRIMER_RIGHT_2_SEQUENCE=AAGGAGGGGATGGAGATGAG PRIMER_INTERNAL_2_SEQUENCE=TGACTTGGCTCTGTCTGTCC PRIMER_LEFT_2=76,20 PRIMER_RIGHT_2=161,20 PRIMER_INTERNAL_2=117,20 PRIMER_LEFT_2_TM=58.429 PRIMER_RIGHT_2_TM=60.412 PRIMER_INTERNAL_2_TM=58.968 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=10.0000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=86 PRIMER_PAIR_3_PENALTY=1.992408 PRIMER_LEFT_3_PENALTY=1.580186 PRIMER_RIGHT_3_PENALTY=0.412222 PRIMER_INTERNAL_3_PENALTY=1.032397 PRIMER_LEFT_3_SEQUENCE=CCCTTGATAATTTCATGCCAG PRIMER_RIGHT_3_SEQUENCE=AAGGAGGGGATGGAGATGAG PRIMER_INTERNAL_3_SEQUENCE=TGACTTGGCTCTGTCTGTCC PRIMER_LEFT_3=76,21 PRIMER_RIGHT_3=161,20 PRIMER_INTERNAL_3=117,20 PRIMER_LEFT_3_TM=59.420 PRIMER_RIGHT_3_TM=60.412 PRIMER_INTERNAL_3_TM=58.968 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=86 PRIMER_PAIR_4_PENALTY=2.106319 PRIMER_LEFT_4_PENALTY=1.570745 PRIMER_RIGHT_4_PENALTY=0.535575 PRIMER_INTERNAL_4_PENALTY=1.032397 PRIMER_LEFT_4_SEQUENCE=CCCTTGATAATTTCATGCCA PRIMER_RIGHT_4_SEQUENCE=CAGTGTGTTGGAAAGGAGGG PRIMER_INTERNAL_4_SEQUENCE=TGACTTGGCTCTGTCTGTCC PRIMER_LEFT_4=76,20 PRIMER_RIGHT_4=173,20 PRIMER_INTERNAL_4=117,20 PRIMER_LEFT_4_TM=58.429 PRIMER_RIGHT_4_TM=60.536 PRIMER_INTERNAL_4_TM=58.968 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH1075 SEQUENCE_TEMPLATE=TCTTCATGCTNCAGGTCGACTCTAGAGGATCCCCCTTGAATGAGGAGTNNCTTCTTATGGATTAGCAAAGCAAGTGGTTTCTTGAGATAGAATCTACTCCTGGTGAAGGCACTGTGNNNNTTGTTGAAATGNCAGTAAAAGATATAGAATATTCCATATTCTTAGATGATAAGGCAGTGGCAGGGTTTGAGAGGATTGACTCCAGTTGTGAAAGAAGTTCTACTGTGGGTAAAATGCTATCAAACAGNATCACAGGCTACAGAGAAATATTTTGTGAAAGGAAGAGTCAATCAATATGGCAAAGTTCATTGTTGTCTTATTTTAAGAAATTATCACAGCCATCTCTACCTTCAGCAACCAC SEQUENCE_INCLUDED_REGION=34,327 P3_COMMENT=CL is TGGATTAGCAAAGCAAGTGG P3_COMMENT=CR is TTGCTGAAGGTAGAGATGGC P3_COMMENT=CM is TTCTTGAGATAGAATCTACTCCTGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.456778 PRIMER_LEFT_0_PENALTY=0.243525 PRIMER_RIGHT_0_PENALTY=2.213253 PRIMER_INTERNAL_0_PENALTY=6.677004 PRIMER_LEFT_0_SEQUENCE=ACTCCTGGTGAAGGCACTGT PRIMER_RIGHT_0_SEQUENCE=TCCTCTCAAACCCTGCCAC PRIMER_INTERNAL_0_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA PRIMER_LEFT_0=95,20 PRIMER_RIGHT_0=194,19 PRIMER_INTERNAL_0=151,25 PRIMER_LEFT_0_TM=59.756 PRIMER_RIGHT_0_TM=61.213 PRIMER_INTERNAL_0_TM=58.323 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_INTERNAL_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.1000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=2.573257 PRIMER_LEFT_1_PENALTY=0.243525 PRIMER_RIGHT_1_PENALTY=2.329733 PRIMER_INTERNAL_1_PENALTY=6.677004 PRIMER_LEFT_1_SEQUENCE=ACTCCTGGTGAAGGCACTGT PRIMER_RIGHT_1_SEQUENCE=TCCTCTCAAACCCTGCCA PRIMER_INTERNAL_1_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA PRIMER_LEFT_1=95,20 PRIMER_RIGHT_1=194,18 PRIMER_INTERNAL_1=151,25 PRIMER_LEFT_1_TM=59.756 PRIMER_RIGHT_1_TM=60.330 PRIMER_INTERNAL_1_TM=58.323 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.556 PRIMER_INTERNAL_1_GC_PERCENT=32.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.1000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=3.063587 PRIMER_LEFT_2_PENALTY=0.243525 PRIMER_RIGHT_2_PENALTY=2.820062 PRIMER_INTERNAL_2_PENALTY=6.677004 PRIMER_LEFT_2_SEQUENCE=ACTCCTGGTGAAGGCACTGT PRIMER_RIGHT_2_SEQUENCE=CCTCTCAAACCCTGCCAC PRIMER_INTERNAL_2_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA PRIMER_LEFT_2=95,20 PRIMER_RIGHT_2=193,18 PRIMER_INTERNAL_2=151,25 PRIMER_LEFT_2_TM=59.756 PRIMER_RIGHT_2_TM=59.180 PRIMER_INTERNAL_2_TM=58.323 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_INTERNAL_2_GC_PERCENT=32.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.1000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=3.313830 PRIMER_LEFT_3_PENALTY=1.884424 PRIMER_RIGHT_3_PENALTY=1.429406 PRIMER_INTERNAL_3_PENALTY=6.677004 PRIMER_LEFT_3_SEQUENCE=CTCCTGGTGAAGGCACTGTG PRIMER_RIGHT_3_SEQUENCE=ATCCTCTCAAACCCTGCCAC PRIMER_INTERNAL_3_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA PRIMER_LEFT_3=96,20 PRIMER_RIGHT_3=195,20 PRIMER_INTERNAL_3=151,25 PRIMER_LEFT_3_TM=61.884 PRIMER_RIGHT_3_TM=61.429 PRIMER_INTERNAL_3_TM=58.323 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=32.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=3.316946 PRIMER_LEFT_4_PENALTY=1.887540 PRIMER_RIGHT_4_PENALTY=1.429406 PRIMER_INTERNAL_4_PENALTY=6.677004 PRIMER_LEFT_4_SEQUENCE=TCCTGGTGAAGGCACTGTG PRIMER_RIGHT_4_SEQUENCE=ATCCTCTCAAACCCTGCCAC PRIMER_INTERNAL_4_SEQUENCE=TTCCATATTCTTAGATGATAAGGCA PRIMER_LEFT_4=97,19 PRIMER_RIGHT_4=195,20 PRIMER_INTERNAL_4=151,25 PRIMER_LEFT_4_TM=60.888 PRIMER_RIGHT_4_TM=61.429 PRIMER_INTERNAL_4_TM=58.323 PRIMER_LEFT_4_GC_PERCENT=57.895 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=32.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = SEQUENCE_ID=MH1076 SEQUENCE_TEMPLATE=AGGTGAACGTGTGGTTAAAACTAGGCAATTGGTTAAAAATCAATTTAAAAAACAGGCCTAGAAACAGTGACCACACCTCAAGCAATGATTATCCCTAGCACTCAGATTATGTTCTTGAAATACCATTTTCTGCTTTCAAAAGAAAGACATGAGGGCTTCTTGAAGAAATGGCTGATACCAAGCCTGCAGTGAAAAATGCACATGATGAGCCTGGAACATGTTGTCATATGAGTTAAAAAGGAAG SEQUENCE_INCLUDED_REGION=0,244 P3_COMMENT=CL is TGAACGTGTGGTTAAAACTAGG P3_COMMENT=CR is ACAACATGTTCCAGGCTCAT P3_COMMENT=CM is TTAAAAATCAATTTAAAAAACAGGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.511601 PRIMER_LEFT_0_PENALTY=1.193666 PRIMER_RIGHT_0_PENALTY=0.317935 PRIMER_INTERNAL_0_PENALTY=0.248900 PRIMER_LEFT_0_SEQUENCE=GAGGGCTTCTTGAAGAAATGG PRIMER_RIGHT_0_SEQUENCE=GACAACATGTTCCAGGCTCA PRIMER_INTERNAL_0_SEQUENCE=CCAAGCCTGCAGTGAAAAAT PRIMER_LEFT_0=150,21 PRIMER_RIGHT_0=224,20 PRIMER_INTERNAL_0=177,20 PRIMER_LEFT_0_TM=60.194 PRIMER_RIGHT_0_TM=59.682 PRIMER_INTERNAL_0_TM=60.249 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=1.511601 PRIMER_LEFT_1_PENALTY=1.193666 PRIMER_RIGHT_1_PENALTY=0.317935 PRIMER_INTERNAL_1_PENALTY=0.248900 PRIMER_LEFT_1_SEQUENCE=GAGGGCTTCTTGAAGAAATGG PRIMER_RIGHT_1_SEQUENCE=TGACAACATGTTCCAGGCTC PRIMER_INTERNAL_1_SEQUENCE=CCAAGCCTGCAGTGAAAAAT PRIMER_LEFT_1=150,21 PRIMER_RIGHT_1=225,20 PRIMER_INTERNAL_1=177,20 PRIMER_LEFT_1_TM=60.194 PRIMER_RIGHT_1_TM=59.682 PRIMER_INTERNAL_1_TM=60.249 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=9.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.4000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=1.652107 PRIMER_LEFT_2_PENALTY=1.580170 PRIMER_RIGHT_2_PENALTY=0.071937 PRIMER_INTERNAL_2_PENALTY=0.095771 PRIMER_LEFT_2_SEQUENCE=GACATGAGGGCTTCTTGAAGA PRIMER_RIGHT_2_SEQUENCE=CATGTTCCAGGCTCATCATG PRIMER_INTERNAL_2_SEQUENCE=AATGGCTGATACCAAGCCTG PRIMER_LEFT_2=145,21 PRIMER_RIGHT_2=219,20 PRIMER_INTERNAL_2=166,20 PRIMER_LEFT_2_TM=59.420 PRIMER_RIGHT_2_TM=60.072 PRIMER_INTERNAL_2_TM=60.096 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=7.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=1.769643 PRIMER_LEFT_3_PENALTY=1.697705 PRIMER_RIGHT_3_PENALTY=0.071937 PRIMER_INTERNAL_3_PENALTY=0.095771 PRIMER_LEFT_3_SEQUENCE=TCAAAAGAAAGACATGAGGGC PRIMER_RIGHT_3_SEQUENCE=CATGTTCCAGGCTCATCATG PRIMER_INTERNAL_3_SEQUENCE=AATGGCTGATACCAAGCCTG PRIMER_LEFT_3=135,21 PRIMER_RIGHT_3=219,20 PRIMER_INTERNAL_3=166,20 PRIMER_LEFT_3_TM=59.302 PRIMER_RIGHT_3_TM=60.072 PRIMER_INTERNAL_3_TM=60.096 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=5.00 PRIMER_PAIR_3_PRODUCT_SIZE=85 PRIMER_PAIR_4_PENALTY=1.879457 PRIMER_LEFT_4_PENALTY=1.561523 PRIMER_RIGHT_4_PENALTY=0.317935 PRIMER_INTERNAL_4_PENALTY=0.248900 PRIMER_LEFT_4_SEQUENCE=TGAGGGCTTCTTGAAGAAATG PRIMER_RIGHT_4_SEQUENCE=GACAACATGTTCCAGGCTCA PRIMER_INTERNAL_4_SEQUENCE=CCAAGCCTGCAGTGAAAAAT PRIMER_LEFT_4=149,21 PRIMER_RIGHT_4=224,20 PRIMER_INTERNAL_4=177,20 PRIMER_LEFT_4_TM=59.438 PRIMER_RIGHT_4_TM=59.682 PRIMER_INTERNAL_4_TM=60.249 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=9.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH1080 SEQUENCE_TEMPLATE=CAAGGCACGTCTCAGGGTCCTGGGATCTGGGACAGGTAGGGACATTGCACCAGTCGAAGGGGAATTTTGAGGGTCTGGGGCCCTCCACTTCCGTCCCCCTCCAGCCTTACCCTCCACCTCTGGGTGCTTCATGAGCAGCAAGAAGCCATAGCGCAGGGTGGTGCTGACCGTCTCGGTGCCTGCAATGAAGAGGTTCAACGTGCTCATCATCAGGTTCTTCAAGTAGAACTCCGTNTTGGGGTTCTTCTCCTCCTGCAGGGAGAGGGGGCTTTAGG SEQUENCE_INCLUDED_REGION=0,275 P3_COMMENT=CL is GACAGGTAGGGACATTGCAC P3_COMMENT=CR is ACGGAGTTCTACTTGAAGAACC P3_COMMENT=CM is AGTCGAAGGGGAATTTTGAG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.304879 PRIMER_LEFT_0_PENALTY=0.044122 PRIMER_RIGHT_0_PENALTY=0.260757 PRIMER_INTERNAL_0_PENALTY=2.394220 PRIMER_LEFT_0_SEQUENCE=CAGTCGAAGGGGAATTTTGA PRIMER_RIGHT_0_SEQUENCE=CTCATGAAGCACCCAGAGGT PRIMER_INTERNAL_0_SEQUENCE=CCTCCAGCCTTACCCTCC PRIMER_LEFT_0=50,20 PRIMER_RIGHT_0=134,20 PRIMER_INTERNAL_0=97,18 PRIMER_LEFT_0_TM=60.044 PRIMER_RIGHT_0_TM=60.261 PRIMER_INTERNAL_0_TM=59.606 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=85 PRIMER_PAIR_1_PENALTY=0.339625 PRIMER_LEFT_1_PENALTY=0.044122 PRIMER_RIGHT_1_PENALTY=0.295503 PRIMER_INTERNAL_1_PENALTY=0.639568 PRIMER_LEFT_1_SEQUENCE=CAGTCGAAGGGGAATTTTGA PRIMER_RIGHT_1_SEQUENCE=TCTTGCTGCTCATGAAGCAC PRIMER_INTERNAL_1_SEQUENCE=AGCCTTACCCTCCACCTCTG PRIMER_LEFT_1=50,20 PRIMER_RIGHT_1=142,20 PRIMER_INTERNAL_1=102,20 PRIMER_LEFT_1_TM=60.044 PRIMER_RIGHT_1_TM=60.296 PRIMER_INTERNAL_1_TM=60.640 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=93 PRIMER_PAIR_2_PENALTY=0.373082 PRIMER_LEFT_2_PENALTY=0.010545 PRIMER_RIGHT_2_PENALTY=0.362536 PRIMER_INTERNAL_2_PENALTY=0.255861 PRIMER_LEFT_2_SEQUENCE=AGGTAGGGACATTGCACCAG PRIMER_RIGHT_2_SEQUENCE=ACCCAGAGGTGGAGGGTAAG PRIMER_INTERNAL_2_SEQUENCE=GAAGGGGAATTTTGAGGGTC PRIMER_LEFT_2=33,20 PRIMER_RIGHT_2=124,20 PRIMER_INTERNAL_2=55,20 PRIMER_LEFT_2_TM=59.989 PRIMER_RIGHT_2_TM=60.363 PRIMER_INTERNAL_2_TM=59.744 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=5.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=92 PRIMER_PAIR_3_PENALTY=0.406659 PRIMER_LEFT_3_PENALTY=0.044122 PRIMER_RIGHT_3_PENALTY=0.362536 PRIMER_INTERNAL_3_PENALTY=4.432013 PRIMER_LEFT_3_SEQUENCE=CAGTCGAAGGGGAATTTTGA PRIMER_RIGHT_3_SEQUENCE=ACCCAGAGGTGGAGGGTAAG PRIMER_INTERNAL_3_SEQUENCE=ACTTCCGTCCCCCTCCAG PRIMER_LEFT_3=50,20 PRIMER_RIGHT_3=124,20 PRIMER_INTERNAL_3=86,18 PRIMER_LEFT_3_TM=60.044 PRIMER_RIGHT_3_TM=60.363 PRIMER_INTERNAL_3_TM=62.432 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_GC_PERCENT=66.667 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=5.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=0.420506 PRIMER_LEFT_4_PENALTY=0.260757 PRIMER_RIGHT_4_PENALTY=0.159750 PRIMER_INTERNAL_4_PENALTY=2.261603 PRIMER_LEFT_4_SEQUENCE=ACCTCTGGGTGCTTCATGAG PRIMER_RIGHT_4_SEQUENCE=CGTTGAACCTCTTCATTGCA PRIMER_INTERNAL_4_SEQUENCE=GGTGGTGCTGACCGTCTC PRIMER_LEFT_4=115,20 PRIMER_RIGHT_4=199,20 PRIMER_INTERNAL_4=156,18 PRIMER_LEFT_4_TM=60.261 PRIMER_RIGHT_4_TM=59.840 PRIMER_INTERNAL_4_TM=60.262 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=66.667 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=8.8000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=85 = SEQUENCE_ID=MH1082 SEQUENCE_TEMPLATE=AAACACTGTTATTCAATTTAATTTTGCTTGAAACCAATACACATGTATTTGAAATCATATTGACTGGGAATTTGGATCCTAGCAATATAAATTCCCCACATCACAAAGTACTTGAGGTGCTGAATGAACTAAAATAAAAGATAACTATTTATATTTATTTTNGTTCAATCATAATAATGTATTTAATTTATTATGTTGCAGCCCTAAAGAATTAAAGGGAG SEQUENCE_INCLUDED_REGION=0,221 P3_COMMENT=CL is TAATTTTGCTTGAAACCAATACA P3_COMMENT=CR is CCTTTAATTCTTTAGGGCTGC P3_COMMENT=CM is TGTATTTGAAATCATATTGACTGGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.341381 PRIMER_LEFT_0_PENALTY=0.273480 PRIMER_RIGHT_0_PENALTY=4.067900 PRIMER_INTERNAL_0_PENALTY=2.636481 PRIMER_LEFT_0_SEQUENCE=TTGACTGGGAATTTGGATCC PRIMER_RIGHT_0_SEQUENCE=TTTTAGTTCATTCAGCACCTCAA PRIMER_INTERNAL_0_SEQUENCE=AAATTCCCCACATCACAAAG PRIMER_LEFT_0=59,20 PRIMER_RIGHT_0=133,23 PRIMER_INTERNAL_0=88,20 PRIMER_LEFT_0_TM=59.727 PRIMER_RIGHT_0_TM=58.932 PRIMER_INTERNAL_0_TM=57.364 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=34.783 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.8000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=4.445401 PRIMER_LEFT_1_PENALTY=0.273480 PRIMER_RIGHT_1_PENALTY=4.171921 PRIMER_INTERNAL_1_PENALTY=2.636481 PRIMER_LEFT_1_SEQUENCE=TTGACTGGGAATTTGGATCC PRIMER_RIGHT_1_SEQUENCE=TTTTAGTTCATTCAGCACCTCAAG PRIMER_INTERNAL_1_SEQUENCE=AAATTCCCCACATCACAAAG PRIMER_LEFT_1=59,20 PRIMER_RIGHT_1=133,24 PRIMER_INTERNAL_1=88,20 PRIMER_LEFT_1_TM=59.727 PRIMER_RIGHT_1_TM=59.828 PRIMER_INTERNAL_1_TM=57.364 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=37.500 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.8000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=4.700234 PRIMER_LEFT_2_PENALTY=0.273480 PRIMER_RIGHT_2_PENALTY=4.426754 PRIMER_INTERNAL_2_PENALTY=2.636481 PRIMER_LEFT_2_SEQUENCE=TTGACTGGGAATTTGGATCC PRIMER_RIGHT_2_SEQUENCE=TTTTAGTTCATTCAGCACCTCA PRIMER_INTERNAL_2_SEQUENCE=AAATTCCCCACATCACAAAG PRIMER_LEFT_2=59,20 PRIMER_RIGHT_2=133,22 PRIMER_INTERNAL_2=88,20 PRIMER_LEFT_2_TM=59.727 PRIMER_RIGHT_2_TM=57.573 PRIMER_INTERNAL_2_TM=57.364 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=36.364 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.8000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=5.046100 PRIMER_LEFT_3_PENALTY=0.273480 PRIMER_RIGHT_3_PENALTY=4.772620 PRIMER_INTERNAL_3_PENALTY=2.636481 PRIMER_LEFT_3_SEQUENCE=TTGACTGGGAATTTGGATCC PRIMER_RIGHT_3_SEQUENCE=ATTTTAGTTCATTCAGCACCTCAA PRIMER_INTERNAL_3_SEQUENCE=AAATTCCCCACATCACAAAG PRIMER_LEFT_3=59,20 PRIMER_RIGHT_3=134,24 PRIMER_INTERNAL_3=88,20 PRIMER_LEFT_3_TM=59.727 PRIMER_RIGHT_3_TM=59.227 PRIMER_INTERNAL_3_TM=57.364 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.8000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=5.077873 PRIMER_LEFT_4_PENALTY=1.009973 PRIMER_RIGHT_4_PENALTY=4.067900 PRIMER_INTERNAL_4_PENALTY=2.636481 PRIMER_LEFT_4_SEQUENCE=ATTGACTGGGAATTTGGATCC PRIMER_RIGHT_4_SEQUENCE=TTTTAGTTCATTCAGCACCTCAA PRIMER_INTERNAL_4_SEQUENCE=AAATTCCCCACATCACAAAG PRIMER_LEFT_4=58,21 PRIMER_RIGHT_4=133,23 PRIMER_INTERNAL_4=88,20 PRIMER_LEFT_4_TM=60.010 PRIMER_RIGHT_4_TM=58.932 PRIMER_INTERNAL_4_TM=57.364 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=34.783 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.8000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH1083 SEQUENCE_TEMPLATE=TTGTTCAAGGAGACATATACAAAGATGTATATTTTTGAGTTGTTAATTGTTACAAGGCTAAGAAAATCTTATTGTCAAACAGCAGGGGGATGAAAAAATACACTGTTGTTTGTTGGTCCAGCAGAATGCCTAAAATGAAAATGAATGAATTGGATTTGAATGTATCAACATAGCAAGTCTCAGAGATATAATGTTGTGGCTAAAAAAAAAAAAAGTTGAATATAGNGTATCAANCATTTAGGAGTTTATC SEQUENCE_INCLUDED_REGION=0,250 P3_COMMENT=CL is GTTCAAGGAGACATATACAAAGATG P3_COMMENT=CR is TATTCAACTTTTTTTTTTTTTAGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.390337 PRIMER_LEFT_0_PENALTY=1.376703 PRIMER_RIGHT_0_PENALTY=3.013634 PRIMER_INTERNAL_0_PENALTY=2.022362 PRIMER_LEFT_0_SEQUENCE=TTATTGTCAAACAGCAGGGG PRIMER_RIGHT_0_SEQUENCE=CATTTTCATTTTAGGCATTCTGC PRIMER_INTERNAL_0_SEQUENCE=CACTGTTGTTTGTTGGTCCA PRIMER_LEFT_0=68,20 PRIMER_RIGHT_0=142,23 PRIMER_INTERNAL_0=100,20 PRIMER_LEFT_0_TM=58.623 PRIMER_RIGHT_0_TM=59.986 PRIMER_INTERNAL_0_TM=57.978 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=34.783 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=4.395781 PRIMER_LEFT_1_PENALTY=1.382147 PRIMER_RIGHT_1_PENALTY=3.013634 PRIMER_INTERNAL_1_PENALTY=2.022362 PRIMER_LEFT_1_SEQUENCE=CTTATTGTCAAACAGCAGGGG PRIMER_RIGHT_1_SEQUENCE=CATTTTCATTTTAGGCATTCTGC PRIMER_INTERNAL_1_SEQUENCE=CACTGTTGTTTGTTGGTCCA PRIMER_LEFT_1=67,21 PRIMER_RIGHT_1=142,23 PRIMER_INTERNAL_1=100,20 PRIMER_LEFT_1_TM=59.618 PRIMER_RIGHT_1_TM=59.986 PRIMER_INTERNAL_1_TM=57.978 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=34.783 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=10.9000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=5.045595 PRIMER_LEFT_2_PENALTY=0.883387 PRIMER_RIGHT_2_PENALTY=4.162208 PRIMER_INTERNAL_2_PENALTY=0.213506 PRIMER_LEFT_2_SEQUENCE=TATTGTCAAACAGCAGGGGG PRIMER_RIGHT_2_SEQUENCE=CAAATCCAATTCATTCATTTTCA PRIMER_INTERNAL_2_SEQUENCE=GGTCCAGCAGAATGCCTAAA PRIMER_LEFT_2=69,20 PRIMER_RIGHT_2=157,23 PRIMER_INTERNAL_2=114,20 PRIMER_LEFT_2_TM=60.883 PRIMER_RIGHT_2_TM=58.838 PRIMER_INTERNAL_2_TM=60.214 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=26.087 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=12.4000 PRIMER_RIGHT_2_END_STABILITY=7.3000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=89 PRIMER_PAIR_3_PENALTY=5.171308 PRIMER_LEFT_3_PENALTY=0.213506 PRIMER_RIGHT_3_PENALTY=4.957802 PRIMER_INTERNAL_3_PENALTY=4.162208 PRIMER_LEFT_3_SEQUENCE=GGTCCAGCAGAATGCCTAAA PRIMER_RIGHT_3_SEQUENCE=TTTTTTTTTTTTTAGCCACAACA PRIMER_INTERNAL_3_SEQUENCE=TGAAAATGAATGAATTGGATTTG PRIMER_LEFT_3=114,20 PRIMER_RIGHT_3=213,23 PRIMER_INTERNAL_3=135,23 PRIMER_LEFT_3_TM=60.214 PRIMER_RIGHT_3_TM=58.042 PRIMER_INTERNAL_3_TM=58.838 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=21.739 PRIMER_INTERNAL_3_GC_PERCENT=26.087 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.3000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=5.218852 PRIMER_LEFT_4_PENALTY=0.883387 PRIMER_RIGHT_4_PENALTY=4.335465 PRIMER_INTERNAL_4_PENALTY=0.277985 PRIMER_LEFT_4_SEQUENCE=TATTGTCAAACAGCAGGGGG PRIMER_RIGHT_4_SEQUENCE=TTCATTCATTTTCATTTTAGGCA PRIMER_INTERNAL_4_SEQUENCE=TGTTTGTTGGTCCAGCAGAA PRIMER_LEFT_4=69,20 PRIMER_RIGHT_4=148,23 PRIMER_INTERNAL_4=106,20 PRIMER_LEFT_4_TM=60.883 PRIMER_RIGHT_4_TM=58.665 PRIMER_INTERNAL_4_TM=60.278 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=26.087 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=7.00 PRIMER_LEFT_4_END_STABILITY=12.4000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH1084 SEQUENCE_TEMPLATE=GTGTGAGAGAGATAGAAACAGAGAGAAGAAGAAAAGAGGCAGATTGGAAGTGTGAAAAAAATGAAAGAAGAAGAAAAAAAGAGTCTAAATATTCAGAAATGTAAGTGCTGCCCTCAACTGTTCTTTACCCACTTAATTCTGCAATTTTGAAAACTAGATTGAATTCCTTTGCAAAACCCTTGCATCATGGATACCCGAGTTAAACCGTTAATTAAAAGACATTAAACATGGCCTGGTGGCAATCTCCACGNCTCCAAGTCAGGAGCAGGAAAGGAAAAACAGCAG SEQUENCE_INCLUDED_REGION=0,285 P3_COMMENT=CL is AGAGGCAGATTGGAAGTGTG P3_COMMENT=CR is CACCAGGCCATGTTTAATGT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.169834 PRIMER_LEFT_0_PENALTY=0.085951 PRIMER_RIGHT_0_PENALTY=0.083883 PRIMER_INTERNAL_0_PENALTY=3.325541 PRIMER_LEFT_0_SEQUENCE=AAGTGCTGCCCTCAACTGTT PRIMER_RIGHT_0_SEQUENCE=GGGTTTTGCAAAGGAATTCA PRIMER_INTERNAL_0_SEQUENCE=CCCACTTAATTCTGCAATTTTG PRIMER_LEFT_0=102,20 PRIMER_RIGHT_0=178,20 PRIMER_INTERNAL_0=127,22 PRIMER_LEFT_0_TM=59.914 PRIMER_RIGHT_0_TM=59.916 PRIMER_INTERNAL_0_TM=58.674 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=36.364 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=0.200855 PRIMER_LEFT_1_PENALTY=0.183145 PRIMER_RIGHT_1_PENALTY=0.017711 PRIMER_INTERNAL_1_PENALTY=1.137415 PRIMER_LEFT_1_SEQUENCE=TGGATACCCGAGTTAAACCG PRIMER_RIGHT_1_SEQUENCE=CTTTCCTGCTCCTGACTTGG PRIMER_INTERNAL_1_SEQUENCE=CATTAAACATGGCCTGGTGG PRIMER_LEFT_1=187,20 PRIMER_RIGHT_1=272,20 PRIMER_INTERNAL_1=219,20 PRIMER_LEFT_1_TM=59.817 PRIMER_RIGHT_1_TM=59.982 PRIMER_INTERNAL_1_TM=61.137 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.9000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=86 PRIMER_PAIR_2_PENALTY=0.200855 PRIMER_LEFT_2_PENALTY=0.183145 PRIMER_RIGHT_2_PENALTY=0.017711 PRIMER_INTERNAL_2_PENALTY=1.137415 PRIMER_LEFT_2_SEQUENCE=TGGATACCCGAGTTAAACCG PRIMER_RIGHT_2_SEQUENCE=CCTTTCCTGCTCCTGACTTG PRIMER_INTERNAL_2_SEQUENCE=CATTAAACATGGCCTGGTGG PRIMER_LEFT_2=187,20 PRIMER_RIGHT_2=273,20 PRIMER_INTERNAL_2=219,20 PRIMER_LEFT_2_TM=59.817 PRIMER_RIGHT_2_TM=59.982 PRIMER_INTERNAL_2_TM=61.137 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.9000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=87 PRIMER_PAIR_3_PENALTY=0.238063 PRIMER_LEFT_3_PENALTY=0.220352 PRIMER_RIGHT_3_PENALTY=0.017711 PRIMER_INTERNAL_3_PENALTY=1.137415 PRIMER_LEFT_3_SEQUENCE=GCATCATGGATACCCGAGTT PRIMER_RIGHT_3_SEQUENCE=CTTTCCTGCTCCTGACTTGG PRIMER_INTERNAL_3_SEQUENCE=CATTAAACATGGCCTGGTGG PRIMER_LEFT_3=181,20 PRIMER_RIGHT_3=272,20 PRIMER_INTERNAL_3=219,20 PRIMER_LEFT_3_TM=59.780 PRIMER_RIGHT_3_TM=59.982 PRIMER_INTERNAL_3_TM=61.137 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=92 PRIMER_PAIR_4_PENALTY=0.238063 PRIMER_LEFT_4_PENALTY=0.220352 PRIMER_RIGHT_4_PENALTY=0.017711 PRIMER_INTERNAL_4_PENALTY=1.137415 PRIMER_LEFT_4_SEQUENCE=GCATCATGGATACCCGAGTT PRIMER_RIGHT_4_SEQUENCE=CCTTTCCTGCTCCTGACTTG PRIMER_INTERNAL_4_SEQUENCE=CATTAAACATGGCCTGGTGG PRIMER_LEFT_4=181,20 PRIMER_RIGHT_4=273,20 PRIMER_INTERNAL_4=219,20 PRIMER_LEFT_4_TM=59.780 PRIMER_RIGHT_4_TM=59.982 PRIMER_INTERNAL_4_TM=61.137 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=93 = SEQUENCE_ID=MH1091 SEQUENCE_TEMPLATE=GGGGATGGAGAACTGCCCTCCTGACTCGCCCTGCCCAGGATCTGAAGTTGAAAGAAACAGAAAATCTTTAAACATGATTCTTATTTTGGTTGTGTGTTGAATCTGTGCCTTCCCTCTGAAAAACTCAGGGTTGCCCCAGGTAGGATTTTCCCCAGAACACCAGGGAGAGCACAAGATCAGGTTCCCAGTGCCAGAATGATGGTCAGGGAGGCATGTAACTGGTCAGACAAGGGCATCTGCCAGTGTAAACAAGTTTCTCATGAACT SEQUENCE_INCLUDED_REGION=0,266 P3_COMMENT=CL is CCCAGGATCTGAAGTTGAAA P3_COMMENT=CR is ATGAGAAACTTGTTTACACTGGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.179195 PRIMER_LEFT_0_PENALTY=0.168650 PRIMER_RIGHT_0_PENALTY=0.010545 PRIMER_INTERNAL_0_PENALTY=0.066262 PRIMER_LEFT_0_SEQUENCE=AGGATTTTCCCCAGAACACC PRIMER_RIGHT_0_SEQUENCE=ACCAGTTACATGCCTCCCTG PRIMER_INTERNAL_0_SEQUENCE=GTTCCCAGTGCCAGAATGAT PRIMER_LEFT_0=141,20 PRIMER_RIGHT_0=222,20 PRIMER_INTERNAL_0=180,20 PRIMER_LEFT_0_TM=60.169 PRIMER_RIGHT_0_TM=59.989 PRIMER_INTERNAL_0_TM=59.934 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=0.197494 PRIMER_LEFT_1_PENALTY=0.186949 PRIMER_RIGHT_1_PENALTY=0.010545 PRIMER_INTERNAL_1_PENALTY=0.066262 PRIMER_LEFT_1_SEQUENCE=GTTGCCCCAGGTAGGATTTT PRIMER_RIGHT_1_SEQUENCE=ACCAGTTACATGCCTCCCTG PRIMER_INTERNAL_1_SEQUENCE=GTTCCCAGTGCCAGAATGAT PRIMER_LEFT_1=129,20 PRIMER_RIGHT_1=222,20 PRIMER_INTERNAL_1=180,20 PRIMER_LEFT_1_TM=60.187 PRIMER_RIGHT_1_TM=59.989 PRIMER_INTERNAL_1_TM=59.934 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=94 PRIMER_PAIR_2_PENALTY=0.263165 PRIMER_LEFT_2_PENALTY=0.196903 PRIMER_RIGHT_2_PENALTY=0.066262 PRIMER_INTERNAL_2_PENALTY=0.168650 PRIMER_LEFT_2_SEQUENCE=ATCTGTGCCTTCCCTCTGAA PRIMER_RIGHT_2_SEQUENCE=ATCATTCTGGCACTGGGAAC PRIMER_INTERNAL_2_SEQUENCE=AGGATTTTCCCCAGAACACC PRIMER_LEFT_2=100,20 PRIMER_RIGHT_2=199,20 PRIMER_INTERNAL_2=141,20 PRIMER_LEFT_2_TM=59.803 PRIMER_RIGHT_2_TM=59.934 PRIMER_INTERNAL_2_TM=60.169 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=0.288459 PRIMER_LEFT_3_PENALTY=0.168650 PRIMER_RIGHT_3_PENALTY=0.119809 PRIMER_INTERNAL_3_PENALTY=0.066262 PRIMER_LEFT_3_SEQUENCE=AGGATTTTCCCCAGAACACC PRIMER_RIGHT_3_SEQUENCE=GATGCCCTTGTCTGACCAGT PRIMER_INTERNAL_3_SEQUENCE=GTTCCCAGTGCCAGAATGAT PRIMER_LEFT_3=141,20 PRIMER_RIGHT_3=236,20 PRIMER_INTERNAL_3=180,20 PRIMER_LEFT_3_TM=60.169 PRIMER_RIGHT_3_TM=60.120 PRIMER_INTERNAL_3_TM=59.934 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=96 PRIMER_PAIR_4_PENALTY=0.355066 PRIMER_LEFT_4_PENALTY=0.288804 PRIMER_RIGHT_4_PENALTY=0.066262 PRIMER_INTERNAL_4_PENALTY=0.168650 PRIMER_LEFT_4_SEQUENCE=CTGAAAAACTCAGGGTTGCC PRIMER_RIGHT_4_SEQUENCE=ATCATTCTGGCACTGGGAAC PRIMER_INTERNAL_4_SEQUENCE=AGGATTTTCCCCAGAACACC PRIMER_LEFT_4=115,20 PRIMER_RIGHT_4=199,20 PRIMER_INTERNAL_4=141,20 PRIMER_LEFT_4_TM=59.711 PRIMER_RIGHT_4_TM=59.934 PRIMER_INTERNAL_4_TM=60.169 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=85 = SEQUENCE_ID=MH1093 SEQUENCE_TEMPLATE=CAGGCTTCTCCATTTTCATGCCTACTTCTGCCTCCTGCTAAGTTGCATCAGTTTCAAACACAAAGGTTAAGATGTGTTACCTGGGTTAAGATGTCTTAGCCAGGGGTCAATCCATGACTATTACACTGTGGGGAAGTCTAGGTGATACTAGAGGGNTCAGGGTGCCTGGGTGACAGGTGATCACTTAGAGGGNTCCTGACAGTGCTTATTTACCANCTGGTACAGAAGTTTTTCAGTATCTTTAAACTGGTATGACTGTCCAGTGCATATTGGCTGACTATCAGCCTGA SEQUENCE_INCLUDED_REGION=0,289 P3_COMMENT=CL is TTTTCATGCCTACTTCTGCC P3_COMMENT=CR is CAGGCTGATAGTCAGCCAAT P3_COMMENT=CM is CCTGCTAAGTTGCATCAGTTT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.088326 PRIMER_LEFT_0_PENALTY=0.015375 PRIMER_RIGHT_0_PENALTY=0.072951 PRIMER_INTERNAL_0_PENALTY=4.280778 PRIMER_LEFT_0_SEQUENCE=GCCTCCTGCTAAGTTGCATC PRIMER_RIGHT_0_SEQUENCE=TGACCCCTGGCTAAGACATC PRIMER_INTERNAL_0_SEQUENCE=AAAGGTTAAGATGTGTTACCTGGG PRIMER_LEFT_0=29,20 PRIMER_RIGHT_0=108,20 PRIMER_INTERNAL_0=61,24 PRIMER_LEFT_0_TM=59.985 PRIMER_RIGHT_0_TM=60.073 PRIMER_INTERNAL_0_TM=59.719 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.1000 PRIMER_RIGHT_0_END_STABILITY=6.3000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=0.434718 PRIMER_LEFT_1_PENALTY=0.361767 PRIMER_RIGHT_1_PENALTY=0.072951 PRIMER_INTERNAL_1_PENALTY=2.345718 PRIMER_LEFT_1_SEQUENCE=TCATGCCTACTTCTGCCTCC PRIMER_RIGHT_1_SEQUENCE=TGACCCCTGGCTAAGACATC PRIMER_INTERNAL_1_SEQUENCE=GCATCAGTTTCAAACACAAAGG PRIMER_LEFT_1=15,20 PRIMER_RIGHT_1=108,20 PRIMER_INTERNAL_1=44,22 PRIMER_LEFT_1_TM=60.362 PRIMER_RIGHT_1_TM=60.073 PRIMER_INTERNAL_1_TM=59.654 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=6.3000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=94 PRIMER_PAIR_2_PENALTY=0.465204 PRIMER_LEFT_2_PENALTY=0.015375 PRIMER_RIGHT_2_PENALTY=0.449829 PRIMER_INTERNAL_2_PENALTY=4.280778 PRIMER_LEFT_2_SEQUENCE=GCCTCCTGCTAAGTTGCATC PRIMER_RIGHT_2_SEQUENCE=TTGACCCCTGGCTAAGACAT PRIMER_INTERNAL_2_SEQUENCE=AAAGGTTAAGATGTGTTACCTGGG PRIMER_LEFT_2=29,20 PRIMER_RIGHT_2=109,20 PRIMER_INTERNAL_2=61,24 PRIMER_LEFT_2_TM=59.985 PRIMER_RIGHT_2_TM=59.550 PRIMER_INTERNAL_2_TM=59.719 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=6.3000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=0.465204 PRIMER_LEFT_3_PENALTY=0.015375 PRIMER_RIGHT_3_PENALTY=0.449829 PRIMER_INTERNAL_3_PENALTY=4.280778 PRIMER_LEFT_3_SEQUENCE=GCCTCCTGCTAAGTTGCATC PRIMER_RIGHT_3_SEQUENCE=ATTGACCCCTGGCTAAGACA PRIMER_INTERNAL_3_SEQUENCE=AAAGGTTAAGATGTGTTACCTGGG PRIMER_LEFT_3=29,20 PRIMER_RIGHT_3=110,20 PRIMER_INTERNAL_3=61,24 PRIMER_LEFT_3_TM=59.985 PRIMER_RIGHT_3_TM=59.550 PRIMER_INTERNAL_3_TM=59.719 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=82 PRIMER_PAIR_4_PENALTY=0.619362 PRIMER_LEFT_4_PENALTY=0.546410 PRIMER_RIGHT_4_PENALTY=0.072951 PRIMER_INTERNAL_4_PENALTY=2.345718 PRIMER_LEFT_4_SEQUENCE=ATGCCTACTTCTGCCTCCTG PRIMER_RIGHT_4_SEQUENCE=TGACCCCTGGCTAAGACATC PRIMER_INTERNAL_4_SEQUENCE=GCATCAGTTTCAAACACAAAGG PRIMER_LEFT_4=17,20 PRIMER_RIGHT_4=108,20 PRIMER_INTERNAL_4=44,22 PRIMER_LEFT_4_TM=59.454 PRIMER_RIGHT_4_TM=60.073 PRIMER_INTERNAL_4_TM=59.654 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.3000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=92 = SEQUENCE_ID=MH1095 SEQUENCE_TEMPLATE=TNNTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTAAGAAATGGAGATTAATTTCTTTAGTAGGAAATGAGGAGCCTTTCATTCATTCAACACATATTTATTGAGTGCTTTCAGTGTGCCAACATCATTCTAAATGATTGGGATACATGAGTNGGAGTNAGTNAAGTGGACAATGGCAGCATTTTAGAAAAGGATTAAGGTAATCAACACTACTTTTTNAGAACAATGNGTCTGTGCCTTAAAAGTGTTTTGTGTGTGTGTGTGTGTTTAGGGGAGATGATCAGGACGATGGGGAGGCGCTATCAGTCAATGTATTGTTTGGGTGAGTGGTGATAAGTGCCTAATACACAAAGCGA SEQUENCE_INCLUDED_REGION=36,323 P3_COMMENT=CL is TTTCTTTAGTAGGAAATGAGGAGC P3_COMMENT=CR is TTTGTGTATTAGGCACTTATCACC P3_COMMENT=CM is CATTCAACACATATTTATTGAGTGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.105706 PRIMER_LEFT_0_PENALTY=0.032351 PRIMER_RIGHT_0_PENALTY=0.073355 PRIMER_INTERNAL_0_PENALTY=5.835450 PRIMER_LEFT_0_SEQUENCE=GAGTGCTTTCAGTGTGCCAA PRIMER_RIGHT_0_SEQUENCE=TAAAATGCTGCCATTGTCCA PRIMER_INTERNAL_0_SEQUENCE=CATTCTAAATGATTGGGATACATGA PRIMER_LEFT_0=105,20 PRIMER_RIGHT_0=188,20 PRIMER_INTERNAL_0=128,25 PRIMER_LEFT_0_TM=60.032 PRIMER_RIGHT_0_TM=60.073 PRIMER_INTERNAL_0_TM=59.165 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=84 PRIMER_PAIR_1_PENALTY=0.105706 PRIMER_LEFT_1_PENALTY=0.032351 PRIMER_RIGHT_1_PENALTY=0.073355 PRIMER_INTERNAL_1_PENALTY=5.491396 PRIMER_LEFT_1_SEQUENCE=TTGAGTGCTTTCAGTGTGCC PRIMER_RIGHT_1_SEQUENCE=TAAAATGCTGCCATTGTCCA PRIMER_INTERNAL_1_SEQUENCE=AACATCATTCTAAATGATTGGGA PRIMER_LEFT_1=103,20 PRIMER_RIGHT_1=188,20 PRIMER_INTERNAL_1=123,23 PRIMER_LEFT_1_TM=60.032 PRIMER_RIGHT_1_TM=60.073 PRIMER_INTERNAL_1_TM=57.509 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=30.435 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=86 PRIMER_PAIR_2_PENALTY=0.383297 PRIMER_LEFT_2_PENALTY=0.309943 PRIMER_RIGHT_2_PENALTY=0.073355 PRIMER_INTERNAL_2_PENALTY=5.835450 PRIMER_LEFT_2_SEQUENCE=TGCTTTCAGTGTGCCAACAT PRIMER_RIGHT_2_SEQUENCE=TAAAATGCTGCCATTGTCCA PRIMER_INTERNAL_2_SEQUENCE=CATTCTAAATGATTGGGATACATGA PRIMER_LEFT_2=108,20 PRIMER_RIGHT_2=188,20 PRIMER_INTERNAL_2=128,25 PRIMER_LEFT_2_TM=60.310 PRIMER_RIGHT_2_TM=60.073 PRIMER_INTERNAL_2_TM=59.165 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=32.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=0.558269 PRIMER_LEFT_3_PENALTY=0.032351 PRIMER_RIGHT_3_PENALTY=0.525918 PRIMER_INTERNAL_3_PENALTY=5.835450 PRIMER_LEFT_3_SEQUENCE=GAGTGCTTTCAGTGTGCCAA PRIMER_RIGHT_3_SEQUENCE=AATGCTGCCATTGTCCACTT PRIMER_INTERNAL_3_SEQUENCE=CATTCTAAATGATTGGGATACATGA PRIMER_LEFT_3=105,20 PRIMER_RIGHT_3=185,20 PRIMER_INTERNAL_3=128,25 PRIMER_LEFT_3_TM=60.032 PRIMER_RIGHT_3_TM=60.526 PRIMER_INTERNAL_3_TM=59.165 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=32.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=10.0000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=81 PRIMER_PAIR_4_PENALTY=0.558269 PRIMER_LEFT_4_PENALTY=0.032351 PRIMER_RIGHT_4_PENALTY=0.525918 PRIMER_INTERNAL_4_PENALTY=5.835450 PRIMER_LEFT_4_SEQUENCE=GAGTGCTTTCAGTGTGCCAA PRIMER_RIGHT_4_SEQUENCE=AAATGCTGCCATTGTCCACT PRIMER_INTERNAL_4_SEQUENCE=CATTCTAAATGATTGGGATACATGA PRIMER_LEFT_4=105,20 PRIMER_RIGHT_4=186,20 PRIMER_INTERNAL_4=128,25 PRIMER_LEFT_4_TM=60.032 PRIMER_RIGHT_4_TM=60.526 PRIMER_INTERNAL_4_TM=59.165 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=32.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=5.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH1096 SEQUENCE_TEMPLATE=ACTCCCAAATCCAGAGATTTGTGTGTGCAAATGTGTATGTNTNAACGTTCAAAATTGAAGACTGTCTAACTCAGCGACCCTCAATCTTGACTGAGCATCAGGGTCATCTAGAG SEQUENCE_INCLUDED_REGION=0,113 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.617746 PRIMER_LEFT_0_PENALTY=1.215635 PRIMER_RIGHT_0_PENALTY=0.402111 PRIMER_INTERNAL_0_PENALTY=1.404189 PRIMER_LEFT_0_SEQUENCE=TCCAGAGATTTGTGTGTGCA PRIMER_RIGHT_0_SEQUENCE=TGACCCTGATGCTCAGTCAA PRIMER_INTERNAL_0_SEQUENCE=TCTAACTCAGCGACCCTCAA PRIMER_LEFT_0=9,20 PRIMER_RIGHT_0=105,20 PRIMER_INTERNAL_0=64,20 PRIMER_LEFT_0_TM=58.784 PRIMER_RIGHT_0_TM=60.402 PRIMER_INTERNAL_0_TM=58.596 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=97 PRIMER_PAIR_1_PENALTY=1.653373 PRIMER_LEFT_1_PENALTY=1.251261 PRIMER_RIGHT_1_PENALTY=0.402111 PRIMER_INTERNAL_1_PENALTY=1.404189 PRIMER_LEFT_1_SEQUENCE=CCAGAGATTTGTGTGTGCAAA PRIMER_RIGHT_1_SEQUENCE=TGACCCTGATGCTCAGTCAA PRIMER_INTERNAL_1_SEQUENCE=TCTAACTCAGCGACCCTCAA PRIMER_LEFT_1=10,21 PRIMER_RIGHT_1=105,20 PRIMER_INTERNAL_1=64,20 PRIMER_LEFT_1_TM=59.749 PRIMER_RIGHT_1_TM=60.402 PRIMER_INTERNAL_1_TM=58.596 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=1.696063 PRIMER_LEFT_2_PENALTY=1.293952 PRIMER_RIGHT_2_PENALTY=0.402111 PRIMER_INTERNAL_2_PENALTY=1.404189 PRIMER_LEFT_2_SEQUENCE=TCCAGAGATTTGTGTGTGCAA PRIMER_RIGHT_2_SEQUENCE=TGACCCTGATGCTCAGTCAA PRIMER_INTERNAL_2_SEQUENCE=TCTAACTCAGCGACCCTCAA PRIMER_LEFT_2=9,21 PRIMER_RIGHT_2=105,20 PRIMER_INTERNAL_2=64,20 PRIMER_LEFT_2_TM=60.294 PRIMER_RIGHT_2_TM=60.402 PRIMER_INTERNAL_2_TM=58.596 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.8000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=97 PRIMER_PAIR_3_PENALTY=2.004027 PRIMER_LEFT_3_PENALTY=1.215635 PRIMER_RIGHT_3_PENALTY=0.788392 PRIMER_INTERNAL_3_PENALTY=1.105717 PRIMER_LEFT_3_SEQUENCE=TCCAGAGATTTGTGTGTGCA PRIMER_RIGHT_3_SEQUENCE=ATGACCCTGATGCTCAGTCA PRIMER_INTERNAL_3_SEQUENCE=AACTCAGCGACCCTCAATCT PRIMER_LEFT_3=9,20 PRIMER_RIGHT_3=106,20 PRIMER_INTERNAL_3=67,20 PRIMER_LEFT_3_TM=58.784 PRIMER_RIGHT_3_TM=59.212 PRIMER_INTERNAL_3_TM=58.894 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=2.016826 PRIMER_LEFT_4_PENALTY=1.215635 PRIMER_RIGHT_4_PENALTY=0.801192 PRIMER_INTERNAL_4_PENALTY=5.525458 PRIMER_LEFT_4_SEQUENCE=TCCAGAGATTTGTGTGTGCA PRIMER_RIGHT_4_SEQUENCE=GTCAAGATTGAGGGTCGCTG PRIMER_INTERNAL_4_SEQUENCE=AACGTTCAAAATTGAAGACTGTC PRIMER_LEFT_4=9,20 PRIMER_RIGHT_4=90,20 PRIMER_INTERNAL_4=43,23 PRIMER_LEFT_4_TM=58.784 PRIMER_RIGHT_4_TM=60.801 PRIMER_INTERNAL_4_TM=57.475 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=34.783 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=10.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH1103 SEQUENCE_TEMPLATE=TCTGCATCCATCTATTTTTCAGATAAAACCATGGGAAGCATTTAACGTNCTAGTAAGTNTAAACAGTAAAATTTCCTTCCTCTGCTTTTCTCTTCTAATAACAAACAATAACTTAAACAGTTACACAAATACATATTCTTACAATAAAAATAGTTTATAATAACACTGTCCCAATAAAGACCACTATTAACTCTTACATCAAAAGGGAGTGGAGAAGAAAAATTTATATTAATAACTCCTTATCTCCCCAACTCTCCACCTCCCCTCAAAAAAAACCTCTGTGGAAAATTAATGTAATTAAG SEQUENCE_INCLUDED_REGION=0,302 P3_COMMENT=CL is TCTGCATCCATCTATTTTTCA P3_COMMENT=CR is TAATTACATTAATTTTCCACAGAGG P3_COMMENT=CM is ATAAAACCATGGGAAGCATTT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.671902 PRIMER_LEFT_0_PENALTY=2.105078 PRIMER_RIGHT_0_PENALTY=0.566824 PRIMER_INTERNAL_0_PENALTY=1.440473 PRIMER_LEFT_0_SEQUENCE=TCAAAAGGGAGTGGAGAAGAA PRIMER_RIGHT_0_SEQUENCE=AGGTTTTTTTTGAGGGGAGG PRIMER_INTERNAL_0_SEQUENCE=CCTTATCTCCCCAACTCTCCA PRIMER_LEFT_0=198,21 PRIMER_RIGHT_0=277,20 PRIMER_INTERNAL_0=237,21 PRIMER_LEFT_0_TM=58.895 PRIMER_RIGHT_0_TM=59.433 PRIMER_INTERNAL_0_TM=60.440 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=1.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=2.780399 PRIMER_LEFT_1_PENALTY=2.213575 PRIMER_RIGHT_1_PENALTY=0.566824 PRIMER_INTERNAL_1_PENALTY=1.440473 PRIMER_LEFT_1_SEQUENCE=TCAAAAGGGAGTGGAGAAGAAA PRIMER_RIGHT_1_SEQUENCE=AGGTTTTTTTTGAGGGGAGG PRIMER_INTERNAL_1_SEQUENCE=CCTTATCTCCCCAACTCTCCA PRIMER_LEFT_1=198,22 PRIMER_RIGHT_1=277,20 PRIMER_INTERNAL_1=237,21 PRIMER_LEFT_1_TM=60.214 PRIMER_RIGHT_1_TM=59.433 PRIMER_INTERNAL_1_TM=60.440 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=2.791146 PRIMER_LEFT_2_PENALTY=2.224322 PRIMER_RIGHT_2_PENALTY=0.566824 PRIMER_INTERNAL_2_PENALTY=1.440473 PRIMER_LEFT_2_SEQUENCE=CATCAAAAGGGAGTGGAGAAG PRIMER_RIGHT_2_SEQUENCE=AGGTTTTTTTTGAGGGGAGG PRIMER_INTERNAL_2_SEQUENCE=CCTTATCTCCCCAACTCTCCA PRIMER_LEFT_2=196,21 PRIMER_RIGHT_2=277,20 PRIMER_INTERNAL_2=237,21 PRIMER_LEFT_2_TM=58.776 PRIMER_RIGHT_2_TM=59.433 PRIMER_INTERNAL_2_TM=60.440 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=82 PRIMER_PAIR_3_PENALTY=2.801991 PRIMER_LEFT_3_PENALTY=2.105078 PRIMER_RIGHT_3_PENALTY=0.696913 PRIMER_INTERNAL_3_PENALTY=1.478252 PRIMER_LEFT_3_SEQUENCE=TCAAAAGGGAGTGGAGAAGAA PRIMER_RIGHT_3_SEQUENCE=GGTTTTTTTTGAGGGGAGGT PRIMER_INTERNAL_3_SEQUENCE=TCCTTATCTCCCCAACTCTCC PRIMER_LEFT_3=198,21 PRIMER_RIGHT_3=276,20 PRIMER_INTERNAL_3=236,21 PRIMER_LEFT_3_TM=58.895 PRIMER_RIGHT_3_TM=59.303 PRIMER_INTERNAL_3_TM=59.522 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=1.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=2.831268 PRIMER_LEFT_4_PENALTY=2.264444 PRIMER_RIGHT_4_PENALTY=0.566824 PRIMER_INTERNAL_4_PENALTY=1.440473 PRIMER_LEFT_4_SEQUENCE=CAAAAGGGAGTGGAGAAGAAAA PRIMER_RIGHT_4_SEQUENCE=AGGTTTTTTTTGAGGGGAGG PRIMER_INTERNAL_4_SEQUENCE=CCTTATCTCCCCAACTCTCCA PRIMER_LEFT_4=199,22 PRIMER_RIGHT_4=277,20 PRIMER_INTERNAL_4=237,21 PRIMER_LEFT_4_TM=59.736 PRIMER_RIGHT_4_TM=59.433 PRIMER_INTERNAL_4_TM=60.440 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH1109 SEQUENCE_TEMPLATE=NTTTTNNNATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGACCCTAGAGTGTCTTCTGAATAAACCATACCAAGAAGAAAAAATCTATGATGACACAGTCTTGGTTTCTCTACTGGGACATTGCTAAGATGTGGGTTTCCAATAAAAGGATATTGCTACCTTCAATATGAGGCCTAGTTAACTGACCAACCCCACTGAGTAAGCATCTTTTGACATTTCACTGATTTCAGGACTAGAAAAAATGGAAAGTTCACAGTCCCTAGAAATCTTCTTCTAGTCTCAGTGTAAATCTTATATATTTGATGGGATGTTTCAAACTTCTCCCATAAATGAA SEQUENCE_INCLUDED_REGION=38,300 P3_COMMENT=CL is GAGTGTCTTCTGAATAAACCATACC P3_COMMENT=CR is TTCTAGGGACTGTGAACTTTCC P3_COMMENT=CM is AAAATCTATGATGACACAGTCTTGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.879014 PRIMER_LEFT_0_PENALTY=0.877035 PRIMER_RIGHT_0_PENALTY=0.001978 PRIMER_INTERNAL_0_PENALTY=1.804465 PRIMER_LEFT_0_SEQUENCE=TGCTAAGATGTGGGTTTCCA PRIMER_RIGHT_0_SEQUENCE=CTCAGTGGGGTTGGTCAGTT PRIMER_INTERNAL_0_SEQUENCE=TGCTACCTTCAATATGAGGCC PRIMER_LEFT_0=125,20 PRIMER_RIGHT_0=202,20 PRIMER_INTERNAL_0=157,21 PRIMER_LEFT_0_TM=59.123 PRIMER_RIGHT_0_TM=60.002 PRIMER_INTERNAL_0_TM=59.196 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 PRIMER_PAIR_1_PENALTY=1.426314 PRIMER_LEFT_1_PENALTY=0.877035 PRIMER_RIGHT_1_PENALTY=0.549279 PRIMER_INTERNAL_1_PENALTY=1.804465 PRIMER_LEFT_1_SEQUENCE=TGCTAAGATGTGGGTTTCCA PRIMER_RIGHT_1_SEQUENCE=ACTCAGTGGGGTTGGTCAGT PRIMER_INTERNAL_1_SEQUENCE=TGCTACCTTCAATATGAGGCC PRIMER_LEFT_1=125,20 PRIMER_RIGHT_1=203,20 PRIMER_INTERNAL_1=157,21 PRIMER_LEFT_1_TM=59.123 PRIMER_RIGHT_1_TM=59.451 PRIMER_INTERNAL_1_TM=59.196 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=79 PRIMER_PAIR_2_PENALTY=1.489075 PRIMER_LEFT_2_PENALTY=1.487097 PRIMER_RIGHT_2_PENALTY=0.001978 PRIMER_INTERNAL_2_PENALTY=1.804465 PRIMER_LEFT_2_SEQUENCE=TGCTAAGATGTGGGTTTCCAA PRIMER_RIGHT_2_SEQUENCE=CTCAGTGGGGTTGGTCAGTT PRIMER_INTERNAL_2_SEQUENCE=TGCTACCTTCAATATGAGGCC PRIMER_LEFT_2=125,21 PRIMER_RIGHT_2=202,20 PRIMER_INTERNAL_2=157,21 PRIMER_LEFT_2_TM=60.487 PRIMER_RIGHT_2_TM=60.002 PRIMER_INTERNAL_2_TM=59.196 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=78 PRIMER_PAIR_3_PENALTY=1.489075 PRIMER_LEFT_3_PENALTY=1.487097 PRIMER_RIGHT_3_PENALTY=0.001978 PRIMER_INTERNAL_3_PENALTY=1.804465 PRIMER_LEFT_3_SEQUENCE=TTGCTAAGATGTGGGTTTCCA PRIMER_RIGHT_3_SEQUENCE=CTCAGTGGGGTTGGTCAGTT PRIMER_INTERNAL_3_SEQUENCE=TGCTACCTTCAATATGAGGCC PRIMER_LEFT_3=124,21 PRIMER_RIGHT_3=202,20 PRIMER_INTERNAL_3=157,21 PRIMER_LEFT_3_TM=60.487 PRIMER_RIGHT_3_TM=60.002 PRIMER_INTERNAL_3_TM=59.196 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=1.811911 PRIMER_LEFT_4_PENALTY=0.877035 PRIMER_RIGHT_4_PENALTY=0.934876 PRIMER_INTERNAL_4_PENALTY=1.804465 PRIMER_LEFT_4_SEQUENCE=TGCTAAGATGTGGGTTTCCA PRIMER_RIGHT_4_SEQUENCE=GCTTACTCAGTGGGGTTGGT PRIMER_INTERNAL_4_SEQUENCE=TGCTACCTTCAATATGAGGCC PRIMER_LEFT_4=125,20 PRIMER_RIGHT_4=207,20 PRIMER_INTERNAL_4=157,21 PRIMER_LEFT_4_TM=59.123 PRIMER_RIGHT_4_TM=59.065 PRIMER_INTERNAL_4_TM=59.196 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=83 = SEQUENCE_ID=MH1113 SEQUENCE_TEMPLATE=TCACCTTTTCAGGCCCTGGCAGAAGGGTGAAGGCAAAGGCCCCAGACACAGGNNTAGAAGAGATGGNATCTGGNNGCCAATGCAAGACCCACCTTTTACAGATGGGAG SEQUENCE_INCLUDED_REGION=0,108 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.273937 PRIMER_LEFT_0_PENALTY=2.244684 PRIMER_RIGHT_0_PENALTY=0.029253 PRIMER_INTERNAL_0_PENALTY=1.730588 PRIMER_LEFT_0_SEQUENCE=TCACCTTTTCAGGCCCTG PRIMER_RIGHT_0_SEQUENCE=AAAAGGTGGGTCTTGCATTG PRIMER_INTERNAL_0_SEQUENCE=AGAAGGGTGAAGGCAAAGG PRIMER_LEFT_0=0,18 PRIMER_RIGHT_0=96,20 PRIMER_INTERNAL_0=20,19 PRIMER_LEFT_0_TM=59.755 PRIMER_RIGHT_0_TM=59.971 PRIMER_INTERNAL_0_TM=59.269 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=52.632 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=7.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=97 PRIMER_PAIR_1_PENALTY=2.693946 PRIMER_LEFT_1_PENALTY=2.664693 PRIMER_RIGHT_1_PENALTY=0.029253 PRIMER_INTERNAL_1_PENALTY=2.236832 PRIMER_LEFT_1_SEQUENCE=CTGGCAGAAGGGTGAAGG PRIMER_RIGHT_1_SEQUENCE=AAAAGGTGGGTCTTGCATTG PRIMER_INTERNAL_1_SEQUENCE=CAAAGGCCCCAGACACAG PRIMER_LEFT_1=15,18 PRIMER_RIGHT_1=96,20 PRIMER_INTERNAL_1=33,18 PRIMER_LEFT_1_TM=59.335 PRIMER_RIGHT_1_TM=59.971 PRIMER_INTERNAL_1_TM=60.237 PRIMER_LEFT_1_GC_PERCENT=61.111 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=2.693946 PRIMER_LEFT_2_PENALTY=2.664693 PRIMER_RIGHT_2_PENALTY=0.029253 PRIMER_INTERNAL_2_PENALTY=2.236832 PRIMER_LEFT_2_SEQUENCE=CCTGGCAGAAGGGTGAAG PRIMER_RIGHT_2_SEQUENCE=AAAAGGTGGGTCTTGCATTG PRIMER_INTERNAL_2_SEQUENCE=CAAAGGCCCCAGACACAG PRIMER_LEFT_2=14,18 PRIMER_RIGHT_2=96,20 PRIMER_INTERNAL_2=33,18 PRIMER_LEFT_2_TM=59.335 PRIMER_RIGHT_2_TM=59.971 PRIMER_INTERNAL_2_TM=60.237 PRIMER_LEFT_2_GC_PERCENT=61.111 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=7.2000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=3.074875 PRIMER_LEFT_3_PENALTY=2.244684 PRIMER_RIGHT_3_PENALTY=0.830191 PRIMER_INTERNAL_3_PENALTY=1.730588 PRIMER_LEFT_3_SEQUENCE=TCACCTTTTCAGGCCCTG PRIMER_RIGHT_3_SEQUENCE=TGTAAAAGGTGGGTCTTGCA PRIMER_INTERNAL_3_SEQUENCE=AGAAGGGTGAAGGCAAAGG PRIMER_LEFT_3=0,18 PRIMER_RIGHT_3=99,20 PRIMER_INTERNAL_3=20,19 PRIMER_LEFT_3_TM=59.755 PRIMER_RIGHT_3_TM=59.170 PRIMER_INTERNAL_3_TM=59.269 PRIMER_LEFT_3_GC_PERCENT=55.556 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=52.632 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=7.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=8.8000 PRIMER_PAIR_3_COMPL_ANY=8.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=3.262372 PRIMER_LEFT_4_PENALTY=2.244684 PRIMER_RIGHT_4_PENALTY=1.017688 PRIMER_INTERNAL_4_PENALTY=1.730588 PRIMER_LEFT_4_SEQUENCE=TCACCTTTTCAGGCCCTG PRIMER_RIGHT_4_SEQUENCE=TAAAAGGTGGGTCTTGCATTG PRIMER_INTERNAL_4_SEQUENCE=AGAAGGGTGAAGGCAAAGG PRIMER_LEFT_4=0,18 PRIMER_RIGHT_4=97,21 PRIMER_INTERNAL_4=20,19 PRIMER_LEFT_4_TM=59.755 PRIMER_RIGHT_4_TM=59.982 PRIMER_INTERNAL_4_TM=59.269 PRIMER_LEFT_4_GC_PERCENT=55.556 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=52.632 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=7.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=7.2000 PRIMER_PAIR_4_COMPL_ANY=8.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH1123 SEQUENCE_TEMPLATE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTCAAAACTATTAAGGGNATAAATAAAAAGTNGGGCAAGTNCATTTTTGGTTTAAAATGNGACTNTTAAAGCAGAAGGGAGTNTCCTTCCCCCTCCCCCACAAAGAATATTTTATAGATGTGAAAATACTAAGAGATTAAAGTTTCAGCAGAAATAAATATCTCAAAGGAGGCACCATACTATTTCCTTTCCATATGTTTAGTANNTCATTTTCACACATCGTTTGTAGTCTCCTAAGTTTAGACAAATCATCTTAG SEQUENCE_INCLUDED_REGION=34,256 P3_COMMENT=CL is CCTCCCCCACAAAGAATATT P3_COMMENT=CR is AGGAGACTACAAACGATGTGTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.044631 PRIMER_LEFT_0_PENALTY=0.010529 PRIMER_RIGHT_0_PENALTY=2.034102 PRIMER_INTERNAL_0_PENALTY=8.987340 PRIMER_LEFT_0_SEQUENCE=CCCTCCCCCACAAAGAATAT PRIMER_RIGHT_0_SEQUENCE=TGGTGCCTCCTTTGAGATATTT PRIMER_INTERNAL_0_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA PRIMER_LEFT_0=123,20 PRIMER_RIGHT_0=209,22 PRIMER_INTERNAL_0=161,26 PRIMER_LEFT_0_TM=60.011 PRIMER_RIGHT_0_TM=59.966 PRIMER_INTERNAL_0_TM=57.013 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.909 PRIMER_INTERNAL_0_GC_PERCENT=30.769 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=5.8000 PRIMER_RIGHT_0_END_STABILITY=6.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=87 PRIMER_PAIR_1_PENALTY=2.207274 PRIMER_LEFT_1_PENALTY=0.010529 PRIMER_RIGHT_1_PENALTY=2.196745 PRIMER_INTERNAL_1_PENALTY=8.987340 PRIMER_LEFT_1_SEQUENCE=CCCTCCCCCACAAAGAATAT PRIMER_RIGHT_1_SEQUENCE=AGTATGGTGCCTCCTTTGAGA PRIMER_INTERNAL_1_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA PRIMER_LEFT_1=123,20 PRIMER_RIGHT_1=213,21 PRIMER_INTERNAL_1=161,26 PRIMER_LEFT_1_TM=60.011 PRIMER_RIGHT_1_TM=58.803 PRIMER_INTERNAL_1_TM=57.013 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_INTERNAL_1_GC_PERCENT=30.769 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=8.00 PRIMER_LEFT_1_END_STABILITY=5.8000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=2.273455 PRIMER_LEFT_2_PENALTY=0.010529 PRIMER_RIGHT_2_PENALTY=2.262926 PRIMER_INTERNAL_2_PENALTY=8.987340 PRIMER_LEFT_2_SEQUENCE=CCCTCCCCCACAAAGAATAT PRIMER_RIGHT_2_SEQUENCE=GTATGGTGCCTCCTTTGAGA PRIMER_INTERNAL_2_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA PRIMER_LEFT_2=123,20 PRIMER_RIGHT_2=212,20 PRIMER_INTERNAL_2=161,26 PRIMER_LEFT_2_TM=60.011 PRIMER_RIGHT_2_TM=57.737 PRIMER_INTERNAL_2_TM=57.013 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=30.769 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=8.00 PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=2.351561 PRIMER_LEFT_3_PENALTY=0.010529 PRIMER_RIGHT_3_PENALTY=2.341032 PRIMER_INTERNAL_3_PENALTY=8.987340 PRIMER_LEFT_3_SEQUENCE=CCCTCCCCCACAAAGAATAT PRIMER_RIGHT_3_SEQUENCE=TGGTGCCTCCTTTGAGATATT PRIMER_INTERNAL_3_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA PRIMER_LEFT_3=123,20 PRIMER_RIGHT_3=209,21 PRIMER_INTERNAL_3=161,26 PRIMER_LEFT_3_TM=60.011 PRIMER_RIGHT_3_TM=58.659 PRIMER_INTERNAL_3_TM=57.013 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_INTERNAL_3_GC_PERCENT=30.769 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=8.00 PRIMER_LEFT_3_END_STABILITY=5.8000 PRIMER_RIGHT_3_END_STABILITY=5.8000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=5.00 PRIMER_PAIR_3_PRODUCT_SIZE=87 PRIMER_PAIR_4_PENALTY=2.806077 PRIMER_LEFT_4_PENALTY=0.010529 PRIMER_RIGHT_4_PENALTY=2.795548 PRIMER_INTERNAL_4_PENALTY=8.987340 PRIMER_LEFT_4_SEQUENCE=CCCTCCCCCACAAAGAATAT PRIMER_RIGHT_4_SEQUENCE=TGGTGCCTCCTTTGAGATAT PRIMER_INTERNAL_4_SEQUENCE=CTAAGAGATTAAAGTTTCAGCAGAAA PRIMER_LEFT_4=123,20 PRIMER_RIGHT_4=209,20 PRIMER_INTERNAL_4=161,26 PRIMER_LEFT_4_TM=60.011 PRIMER_RIGHT_4_TM=57.204 PRIMER_INTERNAL_4_TM=57.013 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=30.769 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=8.00 PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=5.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH1139 SEQUENCE_TEMPLATE=TTGCNNNCCTCCAGGTCGACTCTAGAGGATCCCCCTTTGTTGTNAAAGCCTCAGTGTNNCTTCAGAGCACAGGGCTTATTTTTACTTTTATGTCCTGACTTNNNNTGGGCCGCCTGAGTCATTCAGAAAAGGTGGCCCAGGGACATGAACACCATTTCTTTTCCTTAATTCCCATAATTAATTGCTTTTAAATTAGGGGCAATGACTCTTTGGCAAATNACCCTGCCAAATACTTGCTTACTCCCCTTTGTGAG SEQUENCE_INCLUDED_REGION=34,220 P3_COMMENT=CL is TTCAGAGCACAGGGCTTATT P3_COMMENT=CR is TAAGCAAGTATTTGGCAGGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.534736 PRIMER_LEFT_0_PENALTY=0.468413 PRIMER_RIGHT_0_PENALTY=0.066323 PRIMER_INTERNAL_0_PENALTY=1.225160 PRIMER_LEFT_0_SEQUENCE=GTCATTCAGAAAAGGTGGCC PRIMER_RIGHT_0_SEQUENCE=CCAAAGAGTCATTGCCCCTA PRIMER_INTERNAL_0_SEQUENCE=CAGGGACATGAACACCATTTC PRIMER_LEFT_0=117,20 PRIMER_RIGHT_0=212,20 PRIMER_INTERNAL_0=137,21 PRIMER_LEFT_0_TM=59.532 PRIMER_RIGHT_0_TM=60.066 PRIMER_INTERNAL_0_TM=60.225 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=11.2000 PRIMER_RIGHT_0_END_STABILITY=8.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=96 PRIMER_PAIR_1_PENALTY=0.700807 PRIMER_LEFT_1_PENALTY=0.634484 PRIMER_RIGHT_1_PENALTY=0.066323 PRIMER_INTERNAL_1_PENALTY=6.124236 PRIMER_LEFT_1_SEQUENCE=CCAGGGACATGAACACCATT PRIMER_RIGHT_1_SEQUENCE=CCAAAGAGTCATTGCCCCTA PRIMER_INTERNAL_1_SEQUENCE=TTTCCTTAATTCCCATAATTAATTGC PRIMER_LEFT_1=136,20 PRIMER_RIGHT_1=212,20 PRIMER_INTERNAL_1=159,26 PRIMER_LEFT_1_TM=60.634 PRIMER_RIGHT_1_TM=60.066 PRIMER_INTERNAL_1_TM=59.876 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=26.923 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.4000 PRIMER_RIGHT_1_END_STABILITY=8.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=1.291483 PRIMER_LEFT_2_PENALTY=1.225160 PRIMER_RIGHT_2_PENALTY=0.066323 PRIMER_INTERNAL_2_PENALTY=6.124236 PRIMER_LEFT_2_SEQUENCE=CAGGGACATGAACACCATTTC PRIMER_RIGHT_2_SEQUENCE=CCAAAGAGTCATTGCCCCTA PRIMER_INTERNAL_2_SEQUENCE=TTTCCTTAATTCCCATAATTAATTGC PRIMER_LEFT_2=137,21 PRIMER_RIGHT_2=212,20 PRIMER_INTERNAL_2=159,26 PRIMER_LEFT_2_TM=60.225 PRIMER_RIGHT_2_TM=60.066 PRIMER_INTERNAL_2_TM=59.876 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=26.923 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=8.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=76 PRIMER_PAIR_3_PENALTY=1.436820 PRIMER_LEFT_3_PENALTY=1.370497 PRIMER_RIGHT_3_PENALTY=0.066323 PRIMER_INTERNAL_3_PENALTY=3.261219 PRIMER_LEFT_3_SEQUENCE=ATTCAGAAAAGGTGGCCCAG PRIMER_RIGHT_3_SEQUENCE=CCAAAGAGTCATTGCCCCTA PRIMER_INTERNAL_3_SEQUENCE=GACATGAACACCATTTCTTTTCC PRIMER_LEFT_3=120,20 PRIMER_RIGHT_3=212,20 PRIMER_INTERNAL_3=141,23 PRIMER_LEFT_3_TM=61.370 PRIMER_RIGHT_3_TM=60.066 PRIMER_INTERNAL_3_TM=59.739 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=8.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=93 PRIMER_PAIR_4_PENALTY=1.511732 PRIMER_LEFT_4_PENALTY=1.445408 PRIMER_RIGHT_4_PENALTY=0.066323 PRIMER_INTERNAL_4_PENALTY=2.095574 PRIMER_LEFT_4_SEQUENCE=ATTCAGAAAAGGTGGCCCA PRIMER_RIGHT_4_SEQUENCE=CCAAAGAGTCATTGCCCCTA PRIMER_INTERNAL_4_SEQUENCE=GGGACATGAACACCATTTCTTT PRIMER_LEFT_4=120,19 PRIMER_RIGHT_4=212,20 PRIMER_INTERNAL_4=139,22 PRIMER_LEFT_4_TM=60.445 PRIMER_RIGHT_4_TM=60.066 PRIMER_INTERNAL_4_TM=60.096 PRIMER_LEFT_4_GC_PERCENT=47.368 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=11.2000 PRIMER_RIGHT_4_END_STABILITY=8.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=93 = SEQUENCE_ID=MH114 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGATTTTTGTTTCAGAGGTTACCAGGAAAATAATTACACCAGTCTGACAATTTTAAATCAAGATTGAAGTATTGCTTTGGTTTCTTCTGAGATTTTGAGTNCCTAAATGAACATATTTGCAAAATTATTAAATATGAAAGTGGGTGGTAGTTTGGTTATTTTTAAAATCCTGAATAAATTTTTATATTTATATGGATTTTGAGGTGTCCCCATTTTATTTTTTCCTCTTTAACTCTGTCATGTTCATTTGCACCCCTTGTTCCCCTGACCTGTA SEQUENCE_INCLUDED_REGION=35,275 P3_COMMENT=CL is CTGATTTTTGTTTCAGAGGTTACC P3_COMMENT=CR is TACAGGTCAGGGGAACAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.776366 PRIMER_LEFT_0_PENALTY=0.735594 PRIMER_RIGHT_0_PENALTY=0.040772 PRIMER_INTERNAL_0_PENALTY=1.438109 PRIMER_LEFT_0_SEQUENCE=TTTTGAGGTGTCCCCATTTT PRIMER_RIGHT_0_SEQUENCE=TACAGGTCAGGGGAACAAGG PRIMER_INTERNAL_0_SEQUENCE=TCTGTCATGTTCATTTGCACC PRIMER_LEFT_0=232,20 PRIMER_RIGHT_0=309,20 PRIMER_INTERNAL_0=269,21 PRIMER_LEFT_0_TM=59.264 PRIMER_RIGHT_0_TM=59.959 PRIMER_INTERNAL_0_TM=59.562 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 PRIMER_PAIR_1_PENALTY=0.990633 PRIMER_LEFT_1_PENALTY=0.949861 PRIMER_RIGHT_1_PENALTY=0.040772 PRIMER_INTERNAL_1_PENALTY=1.438109 PRIMER_LEFT_1_SEQUENCE=GGATTTTGAGGTGTCCCCAT PRIMER_RIGHT_1_SEQUENCE=TACAGGTCAGGGGAACAAGG PRIMER_INTERNAL_1_SEQUENCE=TCTGTCATGTTCATTTGCACC PRIMER_LEFT_1=229,20 PRIMER_RIGHT_1=309,20 PRIMER_INTERNAL_1=269,21 PRIMER_LEFT_1_TM=60.950 PRIMER_RIGHT_1_TM=59.959 PRIMER_INTERNAL_1_TM=59.562 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=81 PRIMER_PAIR_2_PENALTY=0.990633 PRIMER_LEFT_2_PENALTY=0.949861 PRIMER_RIGHT_2_PENALTY=0.040772 PRIMER_INTERNAL_2_PENALTY=1.438109 PRIMER_LEFT_2_SEQUENCE=ATGGATTTTGAGGTGTCCCC PRIMER_RIGHT_2_SEQUENCE=TACAGGTCAGGGGAACAAGG PRIMER_INTERNAL_2_SEQUENCE=TCTGTCATGTTCATTTGCACC PRIMER_LEFT_2=227,20 PRIMER_RIGHT_2=309,20 PRIMER_INTERNAL_2=269,21 PRIMER_LEFT_2_TM=60.950 PRIMER_RIGHT_2_TM=59.959 PRIMER_INTERNAL_2_TM=59.562 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=10.9000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=1.086832 PRIMER_LEFT_3_PENALTY=1.046061 PRIMER_RIGHT_3_PENALTY=0.040772 PRIMER_INTERNAL_3_PENALTY=1.438109 PRIMER_LEFT_3_SEQUENCE=GATTTTGAGGTGTCCCCATTT PRIMER_RIGHT_3_SEQUENCE=TACAGGTCAGGGGAACAAGG PRIMER_INTERNAL_3_SEQUENCE=TCTGTCATGTTCATTTGCACC PRIMER_LEFT_3=230,21 PRIMER_RIGHT_3=309,20 PRIMER_INTERNAL_3=269,21 PRIMER_LEFT_3_TM=60.046 PRIMER_RIGHT_3_TM=59.959 PRIMER_INTERNAL_3_TM=59.562 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=1.349552 PRIMER_LEFT_4_PENALTY=1.308781 PRIMER_RIGHT_4_PENALTY=0.040772 PRIMER_INTERNAL_4_PENALTY=1.438109 PRIMER_LEFT_4_SEQUENCE=GATTTTGAGGTGTCCCCATT PRIMER_RIGHT_4_SEQUENCE=TACAGGTCAGGGGAACAAGG PRIMER_INTERNAL_4_SEQUENCE=TCTGTCATGTTCATTTGCACC PRIMER_LEFT_4=230,20 PRIMER_RIGHT_4=309,20 PRIMER_INTERNAL_4=269,21 PRIMER_LEFT_4_TM=58.691 PRIMER_RIGHT_4_TM=59.959 PRIMER_INTERNAL_4_TM=59.562 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH1146 SEQUENCE_TEMPLATE=CAGGTCGACTCTAGAGGATCCCCTTTGAGTNNACATGCTTATAAGAGATTACATTTATAATACTTATGTGATATTAGTAAATTAAGCCTATTATATAACCAAGCATTAATTAGATTAGAATGCTTTTCTTTAGAATTAAGGATCAGATTATACAGTTGGGCTACAAACTTAGATGTCTGTAGTTGCTGGGCAAGTCAAATCAGCATGTGAAAGGGAAAGATAAGACAGTNTGGGCTGTGGTGGTGTGATAAAACTGGAGAACATGGACCCCTTAGGCCAGCCGCCACACACCAGATTTTACTAAGACACACTGGTGGGGA SEQUENCE_INCLUDED_REGION=23,297 P3_COMMENT=CL is AGAATGCTTTTCTTTAGAATTAAGG P3_COMMENT=CR is CCCCACCAGTGTGTCTTAGT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.568552 PRIMER_LEFT_0_PENALTY=0.259704 PRIMER_RIGHT_0_PENALTY=0.308849 PRIMER_INTERNAL_0_PENALTY=0.580157 PRIMER_LEFT_0_SEQUENCE=AGTTGCTGGGCAAGTCAAAT PRIMER_RIGHT_0_SEQUENCE=TAAGGGGTCCATGTTCTCCA PRIMER_INTERNAL_0_SEQUENCE=GGCTGTGGTGGTGTGATAAA PRIMER_LEFT_0=180,20 PRIMER_RIGHT_0=273,20 PRIMER_INTERNAL_0=232,20 PRIMER_LEFT_0_TM=59.740 PRIMER_RIGHT_0_TM=60.309 PRIMER_INTERNAL_0_TM=59.420 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=94 PRIMER_PAIR_1_PENALTY=0.587237 PRIMER_LEFT_1_PENALTY=0.259704 PRIMER_RIGHT_1_PENALTY=0.327533 PRIMER_INTERNAL_1_PENALTY=0.580157 PRIMER_LEFT_1_SEQUENCE=AGTTGCTGGGCAAGTCAAAT PRIMER_RIGHT_1_SEQUENCE=GCCTAAGGGGTCCATGTTCT PRIMER_INTERNAL_1_SEQUENCE=GGCTGTGGTGGTGTGATAAA PRIMER_LEFT_1=180,20 PRIMER_RIGHT_1=276,20 PRIMER_INTERNAL_1=232,20 PRIMER_LEFT_1_TM=59.740 PRIMER_RIGHT_1_TM=60.328 PRIMER_INTERNAL_1_TM=59.420 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=97 PRIMER_PAIR_2_PENALTY=0.758290 PRIMER_LEFT_2_PENALTY=0.449441 PRIMER_RIGHT_2_PENALTY=0.308849 PRIMER_INTERNAL_2_PENALTY=0.580157 PRIMER_LEFT_2_SEQUENCE=TGTAGTTGCTGGGCAAGTCA PRIMER_RIGHT_2_SEQUENCE=TAAGGGGTCCATGTTCTCCA PRIMER_INTERNAL_2_SEQUENCE=GGCTGTGGTGGTGTGATAAA PRIMER_LEFT_2=177,20 PRIMER_RIGHT_2=273,20 PRIMER_INTERNAL_2=232,20 PRIMER_LEFT_2_TM=60.449 PRIMER_RIGHT_2_TM=60.309 PRIMER_INTERNAL_2_TM=59.420 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=97 PRIMER_PAIR_3_PENALTY=0.776975 PRIMER_LEFT_3_PENALTY=0.449441 PRIMER_RIGHT_3_PENALTY=0.327533 PRIMER_INTERNAL_3_PENALTY=0.580157 PRIMER_LEFT_3_SEQUENCE=TGTAGTTGCTGGGCAAGTCA PRIMER_RIGHT_3_SEQUENCE=GCCTAAGGGGTCCATGTTCT PRIMER_INTERNAL_3_SEQUENCE=GGCTGTGGTGGTGTGATAAA PRIMER_LEFT_3=177,20 PRIMER_RIGHT_3=276,20 PRIMER_INTERNAL_3=232,20 PRIMER_LEFT_3_TM=60.449 PRIMER_RIGHT_3_TM=60.328 PRIMER_INTERNAL_3_TM=59.420 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=0.845267 PRIMER_LEFT_4_PENALTY=0.536418 PRIMER_RIGHT_4_PENALTY=0.308849 PRIMER_INTERNAL_4_PENALTY=0.580157 PRIMER_LEFT_4_SEQUENCE=TAGTTGCTGGGCAAGTCAAA PRIMER_RIGHT_4_SEQUENCE=TAAGGGGTCCATGTTCTCCA PRIMER_INTERNAL_4_SEQUENCE=GGCTGTGGTGGTGTGATAAA PRIMER_LEFT_4=179,20 PRIMER_RIGHT_4=273,20 PRIMER_INTERNAL_4=232,20 PRIMER_LEFT_4_TM=59.464 PRIMER_RIGHT_4_TM=60.309 PRIMER_INTERNAL_4_TM=59.420 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=95 = SEQUENCE_ID=MH1147 SEQUENCE_TEMPLATE=AGGTCGACTCTAGAGGATCCCCCTACTAGGGAGGCTGAGGTGGGAGAATCACTTAAGCCCAGGAGGCAGAGGTTGCAGTGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT SEQUENCE_INCLUDED_REGION=22,60 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH1151 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAATTTGCCAGTNCTGGGTGGGGGCAAATCCTCGAGGGGTNCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=36,37 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH1156 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTATTTACTTAATGGTAACCGTAGTAATAATGGCTATCTTTTAATGGTNNTGGGAGCGCTTAGTNTTTGACCACTTACTGAGTGCTAAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC SEQUENCE_INCLUDED_REGION=36,88 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH1163 SEQUENCE_TEMPLATE=GCNTNCCTCCAGGTCGACTCTAGAGGATCCCCTGGTGTNNNCTGTCACTCTTCCCACTAGCCGGACTACACACAGACAGACTATACACTTCCAAGCCTCCTAATTTTGCCCATGTTGTTCTCTCTACCCAGAATACCTTTCCCTAAGCTGCCTGTAGGCCCTCTCAAAACATGCTAGTTCACTTTGTCAATGCCAGCAGAAGAATCTCTGTTGCTGCTATTAATGTCTTTCAAAGTCTCATGTGCTTTGGTCAGGCCTACCCAGGATAATCTCCATTTTNATTAACTCAAAGTCAACTGATTAGAGACCTTAATTATATCTGAA SEQUENCE_INCLUDED_REGION=32,292 P3_COMMENT=CL is TGTCACTCTTCCCACTAGCC P3_COMMENT=CR is GAGATTATCCTGGGTAGGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.283031 PRIMER_LEFT_0_PENALTY=0.069526 PRIMER_RIGHT_0_PENALTY=0.213506 PRIMER_INTERNAL_0_PENALTY=0.753123 PRIMER_LEFT_0_SEQUENCE=AGCCTCCTAATTTTGCCCAT PRIMER_RIGHT_0_SEQUENCE=GCATGTTTTGAGAGGGCCTA PRIMER_INTERNAL_0_SEQUENCE=ACCTTTCCCTAAGCTGCCTG PRIMER_LEFT_0=93,20 PRIMER_RIGHT_0=173,20 PRIMER_INTERNAL_0=134,20 PRIMER_LEFT_0_TM=59.930 PRIMER_RIGHT_0_TM=60.214 PRIMER_INTERNAL_0_TM=60.753 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.6000 PRIMER_RIGHT_0_END_STABILITY=8.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=0.283031 PRIMER_LEFT_1_PENALTY=0.069526 PRIMER_RIGHT_1_PENALTY=0.213506 PRIMER_INTERNAL_1_PENALTY=0.753123 PRIMER_LEFT_1_SEQUENCE=AGCCTCCTAATTTTGCCCAT PRIMER_RIGHT_1_SEQUENCE=TAGCATGTTTTGAGAGGGCC PRIMER_INTERNAL_1_SEQUENCE=CCTTTCCCTAAGCTGCCTGT PRIMER_LEFT_1=93,20 PRIMER_RIGHT_1=175,20 PRIMER_INTERNAL_1=135,20 PRIMER_LEFT_1_TM=59.930 PRIMER_RIGHT_1_TM=60.214 PRIMER_INTERNAL_1_TM=60.753 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=12.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=0.322471 PRIMER_LEFT_2_PENALTY=0.152999 PRIMER_RIGHT_2_PENALTY=0.169471 PRIMER_INTERNAL_2_PENALTY=4.996479 PRIMER_LEFT_2_SEQUENCE=TTCACTTTGTCAATGCCAGC PRIMER_RIGHT_2_SEQUENCE=CCTGACCAAAGCACATGAGA PRIMER_INTERNAL_2_SEQUENCE=TGTTGCTGCTATTAATGTCTTTCA PRIMER_LEFT_2=177,20 PRIMER_RIGHT_2=254,20 PRIMER_INTERNAL_2=208,24 PRIMER_LEFT_2_TM=59.847 PRIMER_RIGHT_2_TM=59.831 PRIMER_INTERNAL_2_TM=59.004 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=33.333 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=78 PRIMER_PAIR_3_PENALTY=0.382977 PRIMER_LEFT_3_PENALTY=0.213506 PRIMER_RIGHT_3_PENALTY=0.169471 PRIMER_INTERNAL_3_PENALTY=0.012060 PRIMER_LEFT_3_SEQUENCE=GGCCCTCTCAAAACATGCTA PRIMER_RIGHT_3_SEQUENCE=CCTGACCAAAGCACATGAGA PRIMER_INTERNAL_3_SEQUENCE=TTTGTCAATGCCAGCAGAAG PRIMER_LEFT_3=156,20 PRIMER_RIGHT_3=254,20 PRIMER_INTERNAL_3=182,20 PRIMER_LEFT_3_TM=60.214 PRIMER_RIGHT_3_TM=59.831 PRIMER_INTERNAL_3_TM=59.988 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.5000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=0.618913 PRIMER_LEFT_4_PENALTY=0.449441 PRIMER_RIGHT_4_PENALTY=0.169471 PRIMER_INTERNAL_4_PENALTY=4.996479 PRIMER_LEFT_4_SEQUENCE=CACTTTGTCAATGCCAGCAG PRIMER_RIGHT_4_SEQUENCE=CCTGACCAAAGCACATGAGA PRIMER_INTERNAL_4_SEQUENCE=TGTTGCTGCTATTAATGTCTTTCA PRIMER_LEFT_4=179,20 PRIMER_RIGHT_4=254,20 PRIMER_INTERNAL_4=208,24 PRIMER_LEFT_4_TM=60.449 PRIMER_RIGHT_4_TM=59.831 PRIMER_INTERNAL_4_TM=59.004 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH1166 SEQUENCE_TEMPLATE=TGCATCCCTCCAGGTCGACTCTAGAGGATCCCCTTAAAAGTNNACTGCTGACTGATAGGGTNGAAAAGAAAAAAGAATGCCTGGGAGAAGAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTNCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGC SEQUENCE_INCLUDED_REGION=33,60 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH1177 SEQUENCE_TEMPLATE=GACTCTAGAGGATCCCCCTATATAATGGTNAAAATAATGTACAGTTAATGTGACTTGTTACAGGTTTTTACTCTAAGGTNATCACATAAATGAGTNNAGGATTCAAACAATGTTTGTTGTTACTGATGTTGCGAGACTGGCTTCAAGGTTATTTACAGAAGTGGTTCTAGGAAGAGTCATCTTTNGTCAGATGCTGACACCCACTGGTGAAAGATACAAACCTTTCCTAATAGAGGNTCTCTAGACATA SEQUENCE_INCLUDED_REGION=17,232 P3_COMMENT=CL is AGGATTCAAACAATGTTTGTTG P3_COMMENT=CR is GGAAAGGTTTGTATCTTTCACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.085285 PRIMER_LEFT_0_PENALTY=0.955975 PRIMER_RIGHT_0_PENALTY=0.129310 PRIMER_INTERNAL_0_PENALTY=4.902432 PRIMER_LEFT_0_SEQUENCE=GATGTTGCGAGACTGGCTTC PRIMER_RIGHT_0_SEQUENCE=TCTTTCACCAGTGGGTGTCA PRIMER_INTERNAL_0_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA PRIMER_LEFT_0=124,20 PRIMER_RIGHT_0=213,20 PRIMER_INTERNAL_0=155,24 PRIMER_LEFT_0_TM=60.956 PRIMER_RIGHT_0_TM=60.129 PRIMER_INTERNAL_0_TM=59.098 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.833 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=90 PRIMER_PAIR_1_PENALTY=1.245275 PRIMER_LEFT_1_PENALTY=1.115965 PRIMER_RIGHT_1_PENALTY=0.129310 PRIMER_INTERNAL_1_PENALTY=4.902432 PRIMER_LEFT_1_SEQUENCE=GTTGCGAGACTGGCTTCAAG PRIMER_RIGHT_1_SEQUENCE=TCTTTCACCAGTGGGTGTCA PRIMER_INTERNAL_1_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA PRIMER_LEFT_1=127,20 PRIMER_RIGHT_1=213,20 PRIMER_INTERNAL_1=155,24 PRIMER_LEFT_1_TM=61.116 PRIMER_RIGHT_1_TM=60.129 PRIMER_INTERNAL_1_TM=59.098 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=45.833 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=87 PRIMER_PAIR_2_PENALTY=1.257461 PRIMER_LEFT_2_PENALTY=1.128150 PRIMER_RIGHT_2_PENALTY=0.129310 PRIMER_INTERNAL_2_PENALTY=4.902432 PRIMER_LEFT_2_SEQUENCE=GTTGCGAGACTGGCTTCAA PRIMER_RIGHT_2_SEQUENCE=TCTTTCACCAGTGGGTGTCA PRIMER_INTERNAL_2_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA PRIMER_LEFT_2=127,19 PRIMER_RIGHT_2=213,20 PRIMER_INTERNAL_2=155,24 PRIMER_LEFT_2_TM=60.128 PRIMER_RIGHT_2_TM=60.129 PRIMER_INTERNAL_2_TM=59.098 PRIMER_LEFT_2_GC_PERCENT=52.632 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.833 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=87 PRIMER_PAIR_3_PENALTY=1.400001 PRIMER_LEFT_3_PENALTY=1.270690 PRIMER_RIGHT_3_PENALTY=0.129310 PRIMER_INTERNAL_3_PENALTY=4.902432 PRIMER_LEFT_3_SEQUENCE=TTGCGAGACTGGCTTCAAG PRIMER_RIGHT_3_SEQUENCE=TCTTTCACCAGTGGGTGTCA PRIMER_INTERNAL_3_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA PRIMER_LEFT_3=128,19 PRIMER_RIGHT_3=213,20 PRIMER_INTERNAL_3=155,24 PRIMER_LEFT_3_TM=60.271 PRIMER_RIGHT_3_TM=60.129 PRIMER_INTERNAL_3_TM=59.098 PRIMER_LEFT_3_GC_PERCENT=52.632 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=45.833 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=86 PRIMER_PAIR_4_PENALTY=1.551559 PRIMER_LEFT_4_PENALTY=0.955975 PRIMER_RIGHT_4_PENALTY=0.595585 PRIMER_INTERNAL_4_PENALTY=4.902432 PRIMER_LEFT_4_SEQUENCE=GATGTTGCGAGACTGGCTTC PRIMER_RIGHT_4_SEQUENCE=ACCAGTGGGTGTCAGCATCT PRIMER_INTERNAL_4_SEQUENCE=CAGAAGTGGTTCTAGGAAGAGTCA PRIMER_LEFT_4=124,20 PRIMER_RIGHT_4=207,20 PRIMER_INTERNAL_4=155,24 PRIMER_LEFT_4_TM=60.956 PRIMER_RIGHT_4_TM=60.596 PRIMER_INTERNAL_4_TM=59.098 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=45.833 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=84 = SEQUENCE_ID=MH1184 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCTGTTCACATGAGGNNATGAGGACACTATCTTTTCTAGTNAGAAGTGAAGATGTGACAGGTTTTGTTATTGGAATGAGGGAGAACTTCGTAAGATATTCCCTTTTTTCGCCCCTACAAACCCAGCATCTTTAAAACCCCCTGTCTGAAAGCGTGACTCGTCTGTTGCCTTTCCACTCTGGTCAGTGGGTCCTCGAAAACACCATAACATGTTTCAATAATTCTGACAAACCCATGAAGATTTTTTAAAGTTAATGAATCATTCTTTCTCCTGAAAACACATAAAAACACGTTT SEQUENCE_INCLUDED_REGION=35,292 P3_COMMENT=CL is GGAATGAGGGAGAACTTCGT P3_COMMENT=CR is CGTGTTTTTATGTGTTTTCAGG P3_COMMENT=CM is AGATATTCCCTTTTTTCGCC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.398252 PRIMER_LEFT_0_PENALTY=0.248857 PRIMER_RIGHT_0_PENALTY=0.149395 PRIMER_INTERNAL_0_PENALTY=0.475706 PRIMER_LEFT_0_SEQUENCE=GATATTCCCTTTTTTCGCCC PRIMER_RIGHT_0_SEQUENCE=GGAAAGGCAACAGACGAGTC PRIMER_INTERNAL_0_SEQUENCE=AAAACCCCCTGTCTGAAAGC PRIMER_LEFT_0=126,20 PRIMER_RIGHT_0=206,20 PRIMER_INTERNAL_0=165,20 PRIMER_LEFT_0_TM=59.751 PRIMER_RIGHT_0_TM=59.851 PRIMER_INTERNAL_0_TM=60.476 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=12.9000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=0.401799 PRIMER_LEFT_1_PENALTY=0.248857 PRIMER_RIGHT_1_PENALTY=0.152942 PRIMER_INTERNAL_1_PENALTY=0.230410 PRIMER_LEFT_1_SEQUENCE=GATATTCCCTTTTTTCGCCC PRIMER_RIGHT_1_SEQUENCE=AGGACCCACTGACCAGAGTG PRIMER_INTERNAL_1_SEQUENCE=CTGAAAGCGTGACTCGTCTG PRIMER_LEFT_1=126,20 PRIMER_RIGHT_1=225,20 PRIMER_INTERNAL_1=177,20 PRIMER_LEFT_1_TM=59.751 PRIMER_RIGHT_1_TM=60.153 PRIMER_INTERNAL_1_TM=59.770 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=12.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=0.417734 PRIMER_LEFT_2_PENALTY=0.187324 PRIMER_RIGHT_2_PENALTY=0.230410 PRIMER_INTERNAL_2_PENALTY=0.248857 PRIMER_LEFT_2_SEQUENCE=TGGAATGAGGGAGAACTTCG PRIMER_RIGHT_2_SEQUENCE=CGAGTCACGCTTTCAGACAG PRIMER_INTERNAL_2_SEQUENCE=GATATTCCCTTTTTTCGCCC PRIMER_LEFT_2=103,20 PRIMER_RIGHT_2=192,20 PRIMER_INTERNAL_2=126,20 PRIMER_LEFT_2_TM=60.187 PRIMER_RIGHT_2_TM=59.770 PRIMER_INTERNAL_2_TM=59.751 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.7000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=0.417734 PRIMER_LEFT_3_PENALTY=0.187324 PRIMER_RIGHT_3_PENALTY=0.230410 PRIMER_INTERNAL_3_PENALTY=0.248857 PRIMER_LEFT_3_SEQUENCE=TGGAATGAGGGAGAACTTCG PRIMER_RIGHT_3_SEQUENCE=CAGACGAGTCACGCTTTCAG PRIMER_INTERNAL_3_SEQUENCE=GATATTCCCTTTTTTCGCCC PRIMER_LEFT_3=103,20 PRIMER_RIGHT_3=196,20 PRIMER_INTERNAL_3=126,20 PRIMER_LEFT_3_TM=60.187 PRIMER_RIGHT_3_TM=59.770 PRIMER_INTERNAL_3_TM=59.751 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.7000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=0.545579 PRIMER_LEFT_4_PENALTY=0.248857 PRIMER_RIGHT_4_PENALTY=0.296722 PRIMER_INTERNAL_4_PENALTY=0.230410 PRIMER_LEFT_4_SEQUENCE=GATATTCCCTTTTTTCGCCC PRIMER_RIGHT_4_SEQUENCE=GACCAGAGTGGAAAGGCAAC PRIMER_INTERNAL_4_SEQUENCE=CTGTCTGAAAGCGTGACTCG PRIMER_LEFT_4=126,20 PRIMER_RIGHT_4=215,20 PRIMER_INTERNAL_4=173,20 PRIMER_LEFT_4_TM=59.751 PRIMER_RIGHT_4_TM=59.703 PRIMER_INTERNAL_4_TM=59.770 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=12.9000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=90 = SEQUENCE_ID=MH1185 SEQUENCE_TEMPLATE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTAAGTNNAGAATGTTTTTTTTTCAGGAAACCTCTACAATGCAGATGCTAGAGATGAGGGGTACCGAGCTCGAATTCGTAATNATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG SEQUENCE_INCLUDED_REGION=33,57 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH1186 SEQUENCE_TEMPLATE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTCACCTGGTACNNCNGNANTGANTGATCCGGATAAGGACAAAAAACGCCAGAAGAAAAGAGGCATTTTCCCCAAAGTAGCAACAAATATCATGAGAGCATGGCTCTTCCAGCATCTCACAGTAAGTCAAGATGAAAGAGNNAAAAAAAATAGTTTTTGTTGTTTTGCTAGCAAGTTATACGGTCTCATACTGATTTTTTTTTAANCTAAGGTGCCTATTTTCTTATTCTTGAAATCTGATGACTAACAGACTAGTTAAAGTTTTTTTTTTCATCAACTATTACCATCCAA SEQUENCE_INCLUDED_REGION=35,292 P3_COMMENT=CL is TGATCCGGATAAGGACAAAA P3_COMMENT=CR is TCAAGAATAAGAAAATAGGCACC P3_COMMENT=CM is ACGCCAGAAGAAAAGAGGCA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.141189 PRIMER_LEFT_0_PENALTY=0.227356 PRIMER_RIGHT_0_PENALTY=0.913833 PRIMER_INTERNAL_0_PENALTY=0.059875 PRIMER_LEFT_0_SEQUENCE=GGACAAAAAACGCCAGAAGA PRIMER_RIGHT_0_SEQUENCE=TGGAAGAGCCATGCTCTCAT PRIMER_INTERNAL_0_SEQUENCE=AGAGGCATTTTCCCCAAAGT PRIMER_LEFT_0=72,20 PRIMER_RIGHT_0=146,20 PRIMER_INTERNAL_0=94,20 PRIMER_LEFT_0_TM=60.227 PRIMER_RIGHT_0_TM=60.914 PRIMER_INTERNAL_0_TM=59.940 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=1.141189 PRIMER_LEFT_1_PENALTY=0.227356 PRIMER_RIGHT_1_PENALTY=0.913833 PRIMER_INTERNAL_1_PENALTY=0.059875 PRIMER_LEFT_1_SEQUENCE=GGACAAAAAACGCCAGAAGA PRIMER_RIGHT_1_SEQUENCE=GAGATGCTGGAAGAGCCATG PRIMER_INTERNAL_1_SEQUENCE=AGAGGCATTTTCCCCAAAGT PRIMER_LEFT_1=72,20 PRIMER_RIGHT_1=153,20 PRIMER_INTERNAL_1=94,20 PRIMER_LEFT_1_TM=60.227 PRIMER_RIGHT_1_TM=60.914 PRIMER_INTERNAL_1_TM=59.940 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=1.141189 PRIMER_LEFT_2_PENALTY=0.227356 PRIMER_RIGHT_2_PENALTY=0.913833 PRIMER_INTERNAL_2_PENALTY=0.059875 PRIMER_LEFT_2_SEQUENCE=GGACAAAAAACGCCAGAAGA PRIMER_RIGHT_2_SEQUENCE=TGAGATGCTGGAAGAGCCAT PRIMER_INTERNAL_2_SEQUENCE=AGAGGCATTTTCCCCAAAGT PRIMER_LEFT_2=72,20 PRIMER_RIGHT_2=154,20 PRIMER_INTERNAL_2=94,20 PRIMER_LEFT_2_TM=60.227 PRIMER_RIGHT_2_TM=60.914 PRIMER_INTERNAL_2_TM=59.940 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=9.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=1.189582 PRIMER_LEFT_3_PENALTY=0.275749 PRIMER_RIGHT_3_PENALTY=0.913833 PRIMER_INTERNAL_3_PENALTY=0.059875 PRIMER_LEFT_3_SEQUENCE=AAGGACAAAAAACGCCAGAA PRIMER_RIGHT_3_SEQUENCE=GGAAGAGCCATGCTCTCATG PRIMER_INTERNAL_3_SEQUENCE=AGAGGCATTTTCCCCAAAGT PRIMER_LEFT_3=70,20 PRIMER_RIGHT_3=145,20 PRIMER_INTERNAL_3=94,20 PRIMER_LEFT_3_TM=59.724 PRIMER_RIGHT_3_TM=60.914 PRIMER_INTERNAL_3_TM=59.940 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=1.189582 PRIMER_LEFT_4_PENALTY=0.275749 PRIMER_RIGHT_4_PENALTY=0.913833 PRIMER_INTERNAL_4_PENALTY=0.059875 PRIMER_LEFT_4_SEQUENCE=AAGGACAAAAAACGCCAGAA PRIMER_RIGHT_4_SEQUENCE=TGGAAGAGCCATGCTCTCAT PRIMER_INTERNAL_4_SEQUENCE=AGAGGCATTTTCCCCAAAGT PRIMER_LEFT_4=70,20 PRIMER_RIGHT_4=146,20 PRIMER_INTERNAL_4=94,20 PRIMER_LEFT_4_TM=59.724 PRIMER_RIGHT_4_TM=60.914 PRIMER_INTERNAL_4_TM=59.940 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=77 = SEQUENCE_ID=MH1187 SEQUENCE_TEMPLATE=TGCATCCCTCCAGGTCGACTCTAGAGGATCCCCCTAGAAAAGAAATAAGATACTGGGTCATAGGATTGCCCCTGCTTCCTGAAAGCATCCAATCCAGAGTAAAGTTCCATTTCTTTGAGGCTCCTCCCAAATACTCTATCACAAGTNCAAATCCTATAATAAGGCTTTTCTAATGCACTCTCACCGAGAAGCCTTATGGTCCCCATGGTGTGTGTTCTCCCTTGTTGCAAAGACTCAATAAACTGAAACTTGTTCAGTTCCAGGTATTCCTGGTTGTCTTGATTGGAGGGAATTGATAAGTCTATAGGGGTA SEQUENCE_INCLUDED_REGION=33,279 P3_COMMENT=CL is TACTGGGTCATAGGATTGCC P3_COMMENT=CR is TCAATTCCCTCCAATCAAGA P3_COMMENT=CM is CTGCTTCCTGAAAGCATCCA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.370640 PRIMER_LEFT_0_PENALTY=0.273747 PRIMER_RIGHT_0_PENALTY=0.096893 PRIMER_INTERNAL_0_PENALTY=3.115731 PRIMER_LEFT_0_SEQUENCE=GTGTGTTCTCCCTTGTTGCA PRIMER_RIGHT_0_SEQUENCE=TCCCTCCAATCAAGACAACC PRIMER_INTERNAL_0_SEQUENCE=CTGAAACTTGTTCAGTTCCAGG PRIMER_LEFT_0=209,20 PRIMER_RIGHT_0=290,20 PRIMER_INTERNAL_0=242,22 PRIMER_LEFT_0_TM=59.726 PRIMER_RIGHT_0_TM=59.903 PRIMER_INTERNAL_0_TM=58.884 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=0.370640 PRIMER_LEFT_1_PENALTY=0.273747 PRIMER_RIGHT_1_PENALTY=0.096893 PRIMER_INTERNAL_1_PENALTY=3.115731 PRIMER_LEFT_1_SEQUENCE=TGTGTGTTCTCCCTTGTTGC PRIMER_RIGHT_1_SEQUENCE=TCCCTCCAATCAAGACAACC PRIMER_INTERNAL_1_SEQUENCE=CTGAAACTTGTTCAGTTCCAGG PRIMER_LEFT_1=208,20 PRIMER_RIGHT_1=290,20 PRIMER_INTERNAL_1=242,22 PRIMER_LEFT_1_TM=59.726 PRIMER_RIGHT_1_TM=59.903 PRIMER_INTERNAL_1_TM=58.884 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=0.374878 PRIMER_LEFT_2_PENALTY=0.277985 PRIMER_RIGHT_2_PENALTY=0.096893 PRIMER_INTERNAL_2_PENALTY=3.115731 PRIMER_LEFT_2_SEQUENCE=TGTGTTCTCCCTTGTTGCAA PRIMER_RIGHT_2_SEQUENCE=TCCCTCCAATCAAGACAACC PRIMER_INTERNAL_2_SEQUENCE=CTGAAACTTGTTCAGTTCCAGG PRIMER_LEFT_2=210,20 PRIMER_RIGHT_2=290,20 PRIMER_INTERNAL_2=242,22 PRIMER_LEFT_2_TM=60.278 PRIMER_RIGHT_2_TM=59.903 PRIMER_INTERNAL_2_TM=58.884 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=8.8000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=0.518779 PRIMER_LEFT_3_PENALTY=0.348730 PRIMER_RIGHT_3_PENALTY=0.170049 PRIMER_INTERNAL_3_PENALTY=7.082104 PRIMER_LEFT_3_SEQUENCE=ATTTCTTTGAGGCTCCTCCC PRIMER_RIGHT_3_SEQUENCE=CCATAAGGCTTCTCGGTGAG PRIMER_INTERNAL_3_SEQUENCE=TCCTATAATAAGGCTTTTCTAATGCA PRIMER_LEFT_3=108,20 PRIMER_RIGHT_3=198,20 PRIMER_INTERNAL_3=151,26 PRIMER_LEFT_3_TM=59.651 PRIMER_RIGHT_3_TM=59.830 PRIMER_INTERNAL_3_TM=58.918 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=30.769 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=91 PRIMER_PAIR_4_PENALTY=0.530467 PRIMER_LEFT_4_PENALTY=0.360418 PRIMER_RIGHT_4_PENALTY=0.170049 PRIMER_INTERNAL_4_PENALTY=7.082104 PRIMER_LEFT_4_SEQUENCE=TTTGAGGCTCCTCCCAAATA PRIMER_RIGHT_4_SEQUENCE=CCATAAGGCTTCTCGGTGAG PRIMER_INTERNAL_4_SEQUENCE=TCCTATAATAAGGCTTTTCTAATGCA PRIMER_LEFT_4=113,20 PRIMER_RIGHT_4=198,20 PRIMER_INTERNAL_4=151,26 PRIMER_LEFT_4_TM=59.640 PRIMER_RIGHT_4_TM=59.830 PRIMER_INTERNAL_4_TM=58.918 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=30.769 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH1188 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTAGTNTTAAATGTTCTCATGAAATTATTCATTTTCTAAAACAAAGTTTTCCTTCCATGAACCAAACAGGAAAAATCCTTTTCATTCTTCGGTCTCTATGGCCCAAAATTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTG SEQUENCE_INCLUDED_REGION=36,114 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.773284 PRIMER_LEFT_0_PENALTY=3.933413 PRIMER_RIGHT_0_PENALTY=1.839872 PRIMER_INTERNAL_0_PENALTY=2.986824 PRIMER_LEFT_0_SEQUENCE=AAAACAAAGTTTTCCTTCCATGA PRIMER_RIGHT_0_SEQUENCE=TGAATTTTGGGCCATAGAGA PRIMER_INTERNAL_0_SEQUENCE=CCAAACAGGAAAAATCCTTTTC PRIMER_LEFT_0=75,23 PRIMER_RIGHT_0=149,20 PRIMER_INTERNAL_0=99,22 PRIMER_LEFT_0_TM=59.067 PRIMER_RIGHT_0_TM=58.160 PRIMER_INTERNAL_0_TM=59.013 PRIMER_LEFT_0_GC_PERCENT=30.435 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=36.364 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=5.900543 PRIMER_LEFT_1_PENALTY=3.933413 PRIMER_RIGHT_1_PENALTY=1.967130 PRIMER_INTERNAL_1_PENALTY=2.986824 PRIMER_LEFT_1_SEQUENCE=AAAACAAAGTTTTCCTTCCATGA PRIMER_RIGHT_1_SEQUENCE=TGAATTTTGGGCCATAGAGAC PRIMER_INTERNAL_1_SEQUENCE=CCAAACAGGAAAAATCCTTTTC PRIMER_LEFT_1=75,23 PRIMER_RIGHT_1=149,21 PRIMER_INTERNAL_1=99,22 PRIMER_LEFT_1_TM=59.067 PRIMER_RIGHT_1_TM=59.033 PRIMER_INTERNAL_1_TM=59.013 PRIMER_LEFT_1_GC_PERCENT=30.435 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_INTERNAL_1_GC_PERCENT=36.364 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=6.077816 PRIMER_LEFT_2_PENALTY=4.237945 PRIMER_RIGHT_2_PENALTY=1.839872 PRIMER_INTERNAL_2_PENALTY=2.986824 PRIMER_LEFT_2_SEQUENCE=AAAACAAAGTTTTCCTTCCATGAA PRIMER_RIGHT_2_SEQUENCE=TGAATTTTGGGCCATAGAGA PRIMER_INTERNAL_2_SEQUENCE=CCAAACAGGAAAAATCCTTTTC PRIMER_LEFT_2=75,24 PRIMER_RIGHT_2=149,20 PRIMER_INTERNAL_2=99,22 PRIMER_LEFT_2_TM=60.238 PRIMER_RIGHT_2_TM=58.160 PRIMER_INTERNAL_2_TM=59.013 PRIMER_LEFT_2_GC_PERCENT=29.167 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=36.364 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=6.102398 PRIMER_LEFT_3_PENALTY=4.893307 PRIMER_RIGHT_3_PENALTY=1.209091 PRIMER_INTERNAL_3_PENALTY=2.986824 PRIMER_LEFT_3_SEQUENCE=TAAAACAAAGTTTTCCTTCCATGA PRIMER_RIGHT_3_SEQUENCE=GAATTTTGGGCCATAGAGACC PRIMER_INTERNAL_3_SEQUENCE=CCAAACAGGAAAAATCCTTTTC PRIMER_LEFT_3=74,24 PRIMER_RIGHT_3=148,21 PRIMER_INTERNAL_3=99,22 PRIMER_LEFT_3_TM=59.107 PRIMER_RIGHT_3_TM=59.791 PRIMER_INTERNAL_3_TM=59.013 PRIMER_LEFT_3_GC_PERCENT=29.167 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=36.364 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=6.205074 PRIMER_LEFT_4_PENALTY=4.237945 PRIMER_RIGHT_4_PENALTY=1.967130 PRIMER_INTERNAL_4_PENALTY=2.986824 PRIMER_LEFT_4_SEQUENCE=AAAACAAAGTTTTCCTTCCATGAA PRIMER_RIGHT_4_SEQUENCE=TGAATTTTGGGCCATAGAGAC PRIMER_INTERNAL_4_SEQUENCE=CCAAACAGGAAAAATCCTTTTC PRIMER_LEFT_4=75,24 PRIMER_RIGHT_4=149,21 PRIMER_INTERNAL_4=99,22 PRIMER_LEFT_4_TM=60.238 PRIMER_RIGHT_4_TM=59.033 PRIMER_INTERNAL_4_TM=59.013 PRIMER_LEFT_4_GC_PERCENT=29.167 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=36.364 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=6.1000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH119 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGGACAGATTGAGTNTAGAGAAGTGAAAATACCTTCTTCCTCCAGGCCAGGTTAGAGAAAATCAGGCAAAGGAAACAAATTTCAGTGCTGTGTGAAATGGTCTGACATGGACATGGATACCTGTATAGATAAANCCAAGATCTGAACAAAAGTTACCCATTTTGGAGACAGAATGTCCTCCCCCAGAAAGGTTCATGNTAATCCTACCAG SEQUENCE_INCLUDED_REGION=35,212 P3_COMMENT=CL is GGCAAAGGAAACAAATTTCA P3_COMMENT=CR is CATTCTGTCTCCAAAATGGG P3_COMMENT=CM is GTGTGAAATGGTCTGACATGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.864013 PRIMER_LEFT_0_PENALTY=0.815754 PRIMER_RIGHT_0_PENALTY=0.048259 PRIMER_INTERNAL_0_PENALTY=4.261278 PRIMER_LEFT_0_SEQUENCE=ATGGTCTGACATGGACATGG PRIMER_RIGHT_0_SEQUENCE=GGGAGGACATTCTGTCTCCA PRIMER_INTERNAL_0_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC PRIMER_LEFT_0=133,20 PRIMER_RIGHT_0=218,20 PRIMER_INTERNAL_0=171,24 PRIMER_LEFT_0_TM=59.184 PRIMER_RIGHT_0_TM=60.048 PRIMER_INTERNAL_0_TM=60.261 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=86 PRIMER_PAIR_1_PENALTY=1.008672 PRIMER_LEFT_1_PENALTY=0.192918 PRIMER_RIGHT_1_PENALTY=0.815754 PRIMER_INTERNAL_1_PENALTY=2.271131 PRIMER_LEFT_1_SEQUENCE=TCCAGGCCAGGTTAGAGAAA PRIMER_RIGHT_1_SEQUENCE=CCATGTCCATGTCAGACCAT PRIMER_INTERNAL_1_SEQUENCE=GGCAAAGGAAACAAATTTCAG PRIMER_LEFT_1=77,20 PRIMER_RIGHT_1=152,20 PRIMER_INTERNAL_1=101,21 PRIMER_LEFT_1_TM=59.807 PRIMER_RIGHT_1_TM=59.184 PRIMER_INTERNAL_1_TM=58.729 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=7.8000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=1.030658 PRIMER_LEFT_2_PENALTY=0.982399 PRIMER_RIGHT_2_PENALTY=0.048259 PRIMER_INTERNAL_2_PENALTY=4.261278 PRIMER_LEFT_2_SEQUENCE=TGGTCTGACATGGACATGGA PRIMER_RIGHT_2_SEQUENCE=GGGAGGACATTCTGTCTCCA PRIMER_INTERNAL_2_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC PRIMER_LEFT_2=134,20 PRIMER_RIGHT_2=218,20 PRIMER_INTERNAL_2=171,24 PRIMER_LEFT_2_TM=60.982 PRIMER_RIGHT_2_TM=60.048 PRIMER_INTERNAL_2_TM=60.261 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=85 PRIMER_PAIR_3_PENALTY=1.097237 PRIMER_LEFT_3_PENALTY=1.048978 PRIMER_RIGHT_3_PENALTY=0.048259 PRIMER_INTERNAL_3_PENALTY=4.261278 PRIMER_LEFT_3_SEQUENCE=TGAAATGGTCTGACATGGACA PRIMER_RIGHT_3_SEQUENCE=GGGAGGACATTCTGTCTCCA PRIMER_INTERNAL_3_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC PRIMER_LEFT_3=129,21 PRIMER_RIGHT_3=218,20 PRIMER_INTERNAL_3=171,24 PRIMER_LEFT_3_TM=59.951 PRIMER_RIGHT_3_TM=60.048 PRIMER_INTERNAL_3_TM=60.261 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=1.097237 PRIMER_LEFT_4_PENALTY=1.048978 PRIMER_RIGHT_4_PENALTY=0.048259 PRIMER_INTERNAL_4_PENALTY=4.261278 PRIMER_LEFT_4_SEQUENCE=TGTGAAATGGTCTGACATGGA PRIMER_RIGHT_4_SEQUENCE=GGGAGGACATTCTGTCTCCA PRIMER_INTERNAL_4_SEQUENCE=CCAAGATCTGAACAAAAGTTACCC PRIMER_LEFT_4=127,21 PRIMER_RIGHT_4=218,20 PRIMER_INTERNAL_4=171,24 PRIMER_LEFT_4_TM=59.951 PRIMER_RIGHT_4_TM=60.048 PRIMER_INTERNAL_4_TM=60.261 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=92 = SEQUENCE_ID=MH1190 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTATACATCTGTNTATCTTTCTATTTCTCTATCTATCTATAAACTCCAATATACAAATTGGGAGAAAAAGAGTTGTTCTTGTGTTGGTGTGGATAAAGAAGAAAATAGATAGAGAGAAGCCAGTGATAAAAGCAACATCAAAGTAAATTACATTAAATTTCAATAAAATATAANGACCCAAACACACCAGAAACTGCCAATCATATATGACGTCTGCTCTTATTCTCTGCTTTTNGTCTTGTACATTTTCTAAGCAATGGACAATTTCTTCGTCAACAAAATTCCATCTTCTCTCAAATATGCAAG SEQUENCE_INCLUDED_REGION=36,306 P3_COMMENT=CL is TTGTTCTTGTGTTGGTGTGG P3_COMMENT=CR is CGAAGAAATTGTCCATTGCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.247663 PRIMER_LEFT_0_PENALTY=0.812957 PRIMER_RIGHT_0_PENALTY=1.434707 PRIMER_INTERNAL_0_PENALTY=3.629138 PRIMER_LEFT_0_SEQUENCE=AACACACCAGAAACTGCCAA PRIMER_RIGHT_0_SEQUENCE=GACGAAGAAATTGTCCATTGC PRIMER_INTERNAL_0_SEQUENCE=GACGTCTGCTCTTATTCTCTGC PRIMER_LEFT_0=216,20 PRIMER_RIGHT_0=309,21 PRIMER_INTERNAL_0=244,22 PRIMER_LEFT_0_TM=59.187 PRIMER_RIGHT_0_TM=59.565 PRIMER_INTERNAL_0_TM=58.371 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=8.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=94 PRIMER_PAIR_1_PENALTY=2.247663 PRIMER_LEFT_1_PENALTY=0.812957 PRIMER_RIGHT_1_PENALTY=1.434707 PRIMER_INTERNAL_1_PENALTY=3.629138 PRIMER_LEFT_1_SEQUENCE=AAACACACCAGAAACTGCCA PRIMER_RIGHT_1_SEQUENCE=GACGAAGAAATTGTCCATTGC PRIMER_INTERNAL_1_SEQUENCE=GACGTCTGCTCTTATTCTCTGC PRIMER_LEFT_1=215,20 PRIMER_RIGHT_1=309,21 PRIMER_INTERNAL_1=244,22 PRIMER_LEFT_1_TM=59.187 PRIMER_RIGHT_1_TM=59.565 PRIMER_INTERNAL_1_TM=58.371 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=10.0000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=95 PRIMER_PAIR_2_PENALTY=2.247663 PRIMER_LEFT_2_PENALTY=0.812957 PRIMER_RIGHT_2_PENALTY=1.434707 PRIMER_INTERNAL_2_PENALTY=3.629138 PRIMER_LEFT_2_SEQUENCE=CAAACACACCAGAAACTGCC PRIMER_RIGHT_2_SEQUENCE=GACGAAGAAATTGTCCATTGC PRIMER_INTERNAL_2_SEQUENCE=GACGTCTGCTCTTATTCTCTGC PRIMER_LEFT_2=214,20 PRIMER_RIGHT_2=309,21 PRIMER_INTERNAL_2=244,22 PRIMER_LEFT_2_TM=59.187 PRIMER_RIGHT_2_TM=59.565 PRIMER_INTERNAL_2_TM=58.371 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=8.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=2.247663 PRIMER_LEFT_3_PENALTY=0.812957 PRIMER_RIGHT_3_PENALTY=1.434707 PRIMER_INTERNAL_3_PENALTY=3.629138 PRIMER_LEFT_3_SEQUENCE=CCAAACACACCAGAAACTGC PRIMER_RIGHT_3_SEQUENCE=GACGAAGAAATTGTCCATTGC PRIMER_INTERNAL_3_SEQUENCE=GACGTCTGCTCTTATTCTCTGC PRIMER_LEFT_3=213,20 PRIMER_RIGHT_3=309,21 PRIMER_INTERNAL_3=244,22 PRIMER_LEFT_3_TM=59.187 PRIMER_RIGHT_3_TM=59.565 PRIMER_INTERNAL_3_TM=58.371 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=2.295870 PRIMER_LEFT_4_PENALTY=0.861164 PRIMER_RIGHT_4_PENALTY=1.434707 PRIMER_INTERNAL_4_PENALTY=3.629138 PRIMER_LEFT_4_SEQUENCE=ACACCAGAAACTGCCAATCA PRIMER_RIGHT_4_SEQUENCE=GACGAAGAAATTGTCCATTGC PRIMER_INTERNAL_4_SEQUENCE=GACGTCTGCTCTTATTCTCTGC PRIMER_LEFT_4=219,20 PRIMER_RIGHT_4=309,21 PRIMER_INTERNAL_4=244,22 PRIMER_LEFT_4_TM=59.139 PRIMER_RIGHT_4_TM=59.565 PRIMER_INTERNAL_4_TM=58.371 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=8.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH1192 SEQUENCE_TEMPLATE=GCTTGCATNNCTNCAGGTCGACTCTAGAGGATCCCCTGCAGGACTCTTTGAAGAGTAAATTGAACAATGATGTCAGGCACCAAGAAAGACGCCTGGAAGAGACCAGGGCTCCATGAGAGTNAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGA SEQUENCE_INCLUDED_REGION=36,87 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.745299 PRIMER_LEFT_0_PENALTY=4.674205 PRIMER_RIGHT_0_PENALTY=1.071094 PRIMER_INTERNAL_0_PENALTY=0.032945 PRIMER_LEFT_0_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA PRIMER_RIGHT_0_SEQUENCE=GAGCCCTGGTCTCTTCCAG PRIMER_INTERNAL_0_SEQUENCE=ACAATGATGTCAGGCACCAA PRIMER_LEFT_0=36,23 PRIMER_RIGHT_0=110,19 PRIMER_INTERNAL_0=63,20 PRIMER_LEFT_0_TM=58.326 PRIMER_RIGHT_0_TM=59.929 PRIMER_INTERNAL_0_TM=59.967 PRIMER_LEFT_0_GC_PERCENT=39.130 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=10.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=5.881064 PRIMER_LEFT_1_PENALTY=4.674205 PRIMER_RIGHT_1_PENALTY=1.206859 PRIMER_INTERNAL_1_PENALTY=0.032945 PRIMER_LEFT_1_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA PRIMER_RIGHT_1_SEQUENCE=CATGGAGCCCTGGTCTCTT PRIMER_INTERNAL_1_SEQUENCE=ACAATGATGTCAGGCACCAA PRIMER_LEFT_1=36,23 PRIMER_RIGHT_1=114,19 PRIMER_INTERNAL_1=63,20 PRIMER_LEFT_1_TM=58.326 PRIMER_RIGHT_1_TM=60.207 PRIMER_INTERNAL_1_TM=59.967 PRIMER_LEFT_1_GC_PERCENT=39.130 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=10.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.0000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=79 PRIMER_PAIR_2_PENALTY=6.020542 PRIMER_LEFT_2_PENALTY=4.674205 PRIMER_RIGHT_2_PENALTY=1.346336 PRIMER_INTERNAL_2_PENALTY=0.032945 PRIMER_LEFT_2_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA PRIMER_RIGHT_2_SEQUENCE=ACTCTCATGGAGCCCTGGT PRIMER_INTERNAL_2_SEQUENCE=ACAATGATGTCAGGCACCAA PRIMER_LEFT_2=36,23 PRIMER_RIGHT_2=119,19 PRIMER_INTERNAL_2=63,20 PRIMER_LEFT_2_TM=58.326 PRIMER_RIGHT_2_TM=59.654 PRIMER_INTERNAL_2_TM=59.967 PRIMER_LEFT_2_GC_PERCENT=39.130 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=10.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.0000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=9.00 PRIMER_PAIR_2_COMPL_END=7.00 PRIMER_PAIR_2_PRODUCT_SIZE=84 PRIMER_PAIR_3_PENALTY=6.219599 PRIMER_LEFT_3_PENALTY=5.148505 PRIMER_RIGHT_3_PENALTY=1.071094 PRIMER_INTERNAL_3_PENALTY=0.032945 PRIMER_LEFT_3_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAATT PRIMER_RIGHT_3_SEQUENCE=GAGCCCTGGTCTCTTCCAG PRIMER_INTERNAL_3_SEQUENCE=ACAATGATGTCAGGCACCAA PRIMER_LEFT_3=36,25 PRIMER_RIGHT_3=110,19 PRIMER_INTERNAL_3=63,20 PRIMER_LEFT_3_TM=59.851 PRIMER_RIGHT_3_TM=59.929 PRIMER_INTERNAL_3_TM=59.967 PRIMER_LEFT_3_GC_PERCENT=36.000 PRIMER_RIGHT_3_GC_PERCENT=63.158 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=10.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=6.295713 PRIMER_LEFT_4_PENALTY=4.674205 PRIMER_RIGHT_4_PENALTY=1.621508 PRIMER_INTERNAL_4_PENALTY=0.032945 PRIMER_LEFT_4_SEQUENCE=TGCAGGACTCTTTGAAGAGTAAA PRIMER_RIGHT_4_SEQUENCE=ACTCTCATGGAGCCCTGGTC PRIMER_INTERNAL_4_SEQUENCE=ACAATGATGTCAGGCACCAA PRIMER_LEFT_4=36,23 PRIMER_RIGHT_4=119,20 PRIMER_INTERNAL_4=63,20 PRIMER_LEFT_4_TM=58.326 PRIMER_RIGHT_4_TM=61.622 PRIMER_INTERNAL_4_TM=59.967 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=10.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.0000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=9.00 PRIMER_PAIR_4_COMPL_END=6.00 PRIMER_PAIR_4_PRODUCT_SIZE=84 = SEQUENCE_ID=MH1193 SEQUENCE_TEMPLATE=CCTTTTGTNTCCTCATTTGTNAAGTGGGAAANATGGAATCTATCTAAGGCTTGGNAGGTCACATAAAGAAAGGCACTAGAATGATTTCNTTCTNTCTCCTCACCACCCGCTCGACCCTCCCTCTNACTGCCTTNAGGGTTGGCGAAAGANAGGAGACAGGCGAGGTTANGAGATTTNATAGGGCAGGTTTCACAAGGTCAGNGAAATTACATTAANTTAACACTGNCTACATTAAACATGGAATGCCTTTTTGGNGTGTTGGTCCCCACACTT SEQUENCE_INCLUDED_REGION=0,273 P3_COMMENT=CL is ATGGAATCTATCTAAGGCTTGG P3_COMMENT=CR is CAAAAAGGCATTCCATGTTT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.347509 PRIMER_LEFT_0_PENALTY=4.939568 PRIMER_RIGHT_0_PENALTY=0.407941 PRIMER_INTERNAL_0_PENALTY=4.452854 PRIMER_LEFT_0_SEQUENCE=AGGTCACATAAAGAAAGGCACT PRIMER_RIGHT_0_SEQUENCE=ACCTTGTGAAACCTGCCCTA PRIMER_INTERNAL_0_SEQUENCE=TCTCCTCACCACCCGCTC PRIMER_LEFT_0=55,22 PRIMER_RIGHT_0=197,20 PRIMER_INTERNAL_0=94,18 PRIMER_LEFT_0_TM=57.060 PRIMER_RIGHT_0_TM=59.592 PRIMER_INTERNAL_0_TM=62.453 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=8.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=143 PRIMER_PAIR_1_PENALTY=5.478193 PRIMER_LEFT_1_PENALTY=5.070252 PRIMER_RIGHT_1_PENALTY=0.407941 PRIMER_INTERNAL_1_PENALTY=4.452854 PRIMER_LEFT_1_SEQUENCE=GGTCACATAAAGAAAGGCACTAGAA PRIMER_RIGHT_1_SEQUENCE=ACCTTGTGAAACCTGCCCTA PRIMER_INTERNAL_1_SEQUENCE=TCTCCTCACCACCCGCTC PRIMER_LEFT_1=56,25 PRIMER_RIGHT_1=197,20 PRIMER_INTERNAL_1=94,18 PRIMER_LEFT_1_TM=60.070 PRIMER_RIGHT_1_TM=59.592 PRIMER_INTERNAL_1_TM=62.453 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=66.667 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.0000 PRIMER_RIGHT_1_END_STABILITY=8.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=142 PRIMER_PAIR_2_PENALTY=5.518600 PRIMER_LEFT_2_PENALTY=5.110659 PRIMER_RIGHT_2_PENALTY=0.407941 PRIMER_INTERNAL_2_PENALTY=4.452854 PRIMER_LEFT_2_SEQUENCE=GGTCACATAAAGAAAGGCACTAGA PRIMER_RIGHT_2_SEQUENCE=ACCTTGTGAAACCTGCCCTA PRIMER_INTERNAL_2_SEQUENCE=TCTCCTCACCACCCGCTC PRIMER_LEFT_2=56,24 PRIMER_RIGHT_2=197,20 PRIMER_INTERNAL_2=94,18 PRIMER_LEFT_2_TM=58.889 PRIMER_RIGHT_2_TM=59.592 PRIMER_INTERNAL_2_TM=62.453 PRIMER_LEFT_2_GC_PERCENT=41.667 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=66.667 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=5.7000 PRIMER_RIGHT_2_END_STABILITY=8.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=142 PRIMER_PAIR_3_PENALTY=5.629249 PRIMER_LEFT_3_PENALTY=4.939568 PRIMER_RIGHT_3_PENALTY=0.689681 PRIMER_INTERNAL_3_PENALTY=4.452854 PRIMER_LEFT_3_SEQUENCE=AGGTCACATAAAGAAAGGCACT PRIMER_RIGHT_3_SEQUENCE=CTGACCTTGTGAAACCTGCC PRIMER_INTERNAL_3_SEQUENCE=TCTCCTCACCACCCGCTC PRIMER_LEFT_3=55,22 PRIMER_RIGHT_3=200,20 PRIMER_INTERNAL_3=94,18 PRIMER_LEFT_3_TM=57.060 PRIMER_RIGHT_3_TM=60.690 PRIMER_INTERNAL_3_TM=62.453 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=66.667 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=146 PRIMER_PAIR_4_PENALTY=5.656863 PRIMER_LEFT_4_PENALTY=5.248922 PRIMER_RIGHT_4_PENALTY=0.407941 PRIMER_INTERNAL_4_PENALTY=4.452854 PRIMER_LEFT_4_SEQUENCE=AGGTCACATAAAGAAAGGCACTAGA PRIMER_RIGHT_4_SEQUENCE=ACCTTGTGAAACCTGCCCTA PRIMER_INTERNAL_4_SEQUENCE=TCTCCTCACCACCCGCTC PRIMER_LEFT_4=55,25 PRIMER_RIGHT_4=197,20 PRIMER_INTERNAL_4=94,18 PRIMER_LEFT_4_TM=59.751 PRIMER_RIGHT_4_TM=59.592 PRIMER_INTERNAL_4_TM=62.453 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=66.667 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=5.7000 PRIMER_RIGHT_4_END_STABILITY=8.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=143 = SEQUENCE_ID=MH1195 SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTGGGAGCACATGACAGATAACACACTGTCCCTGTTTGTCCCCATCCCTCAGCAGGCATGTTCAAGTTAGGGGCTGGGGGTGAGGAGCAACTNACACNNNTGATCCCAATGGAAGGACCACTGCACTCAGTGCTACAGGAGAGACATAGCAAGATAGGAAAGTGGGAGTGTGTGACTGCTTCTGGCTGGGGGATCAAAAATGGTTTAATGTAGATCTAATTTTTCCTGAGCACCACTTCTATTATCTCATTTAATCTTTATAACAACCATATGGGATAGATATTATCCCCATTTTACAGATGAGAAAACTGGGGCTCCAGAGTAGGGGT SEQUENCE_INCLUDED_REGION=37,331 P3_COMMENT=CL is TGGGAGCACATGACAGATAA P3_COMMENT=CR is GAGCCCCAGTTTTCTCATCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.549252 PRIMER_LEFT_0_PENALTY=0.420772 PRIMER_RIGHT_0_PENALTY=0.128480 PRIMER_INTERNAL_0_PENALTY=4.173280 PRIMER_LEFT_0_SEQUENCE=AATGGAAGGACCACTGCACT PRIMER_RIGHT_0_SEQUENCE=GAAGCAGTCACACACTCCCA PRIMER_INTERNAL_0_SEQUENCE=TGCTACAGGAGAGACATAGCAAG PRIMER_LEFT_0=146,20 PRIMER_RIGHT_0=220,20 PRIMER_INTERNAL_0=169,23 PRIMER_LEFT_0_TM=59.579 PRIMER_RIGHT_0_TM=59.872 PRIMER_INTERNAL_0_TM=58.827 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=47.826 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=0.674645 PRIMER_LEFT_1_PENALTY=0.554836 PRIMER_RIGHT_1_PENALTY=0.119809 PRIMER_INTERNAL_1_PENALTY=3.604007 PRIMER_LEFT_1_SEQUENCE=TCAGCAGGCATGTTCAAGTT PRIMER_RIGHT_1_SEQUENCE=GAGTGCAGTGGTCCTTCCAT PRIMER_INTERNAL_1_SEQUENCE=CTGGGGGTGAGGAGCAACT PRIMER_LEFT_1=87,20 PRIMER_RIGHT_1=166,20 PRIMER_INTERNAL_1=112,19 PRIMER_LEFT_1_TM=59.445 PRIMER_RIGHT_1_TM=60.120 PRIMER_INTERNAL_1_TM=62.604 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=63.158 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=0.703144 PRIMER_LEFT_2_PENALTY=0.574664 PRIMER_RIGHT_2_PENALTY=0.128480 PRIMER_INTERNAL_2_PENALTY=2.184188 PRIMER_LEFT_2_SEQUENCE=ATCCCAATGGAAGGACCACT PRIMER_RIGHT_2_SEQUENCE=GAAGCAGTCACACACTCCCA PRIMER_INTERNAL_2_SEQUENCE=GCACTCAGTGCTACAGGAGAGA PRIMER_LEFT_2=141,20 PRIMER_RIGHT_2=220,20 PRIMER_INTERNAL_2=161,22 PRIMER_LEFT_2_TM=60.575 PRIMER_RIGHT_2_TM=59.872 PRIMER_INTERNAL_2_TM=59.816 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=54.545 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=9.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=0.715749 PRIMER_LEFT_3_PENALTY=0.141085 PRIMER_RIGHT_3_PENALTY=0.574664 PRIMER_INTERNAL_3_PENALTY=0.554836 PRIMER_LEFT_3_SEQUENCE=ACACTGTCCCTGTTTGTCCC PRIMER_RIGHT_3_SEQUENCE=AGTGGTCCTTCCATTGGGAT PRIMER_INTERNAL_3_SEQUENCE=TCAGCAGGCATGTTCAAGTT PRIMER_LEFT_3=61,20 PRIMER_RIGHT_3=160,20 PRIMER_INTERNAL_3=87,20 PRIMER_LEFT_3_TM=59.859 PRIMER_RIGHT_3_TM=60.575 PRIMER_INTERNAL_3_TM=59.445 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.1000 PRIMER_RIGHT_3_END_STABILITY=9.3000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=0.923023 PRIMER_LEFT_4_PENALTY=0.121394 PRIMER_RIGHT_4_PENALTY=0.801629 PRIMER_INTERNAL_4_PENALTY=0.141085 PRIMER_LEFT_4_SEQUENCE=TGTGGGAGCACATGACAGAT PRIMER_RIGHT_4_SEQUENCE=AGCCCCTAACTTGAACATGC PRIMER_INTERNAL_4_SEQUENCE=ACACTGTCCCTGTTTGTCCC PRIMER_LEFT_4=38,20 PRIMER_RIGHT_4=113,20 PRIMER_INTERNAL_4=61,20 PRIMER_LEFT_4_TM=60.121 PRIMER_RIGHT_4_TM=59.198 PRIMER_INTERNAL_4_TM=59.859 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=8.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH1199 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTCTATTTCCCCAATTTCCTATAATTCAATAAAAAGTCAGCAATAACATGTCCAAGGGCATGTTATCTCAAATGTGGATTTTAATGAAACACAAATATGCCCATGCATGTTTTAGCAAATTGTTCTGGACCATGCAAAAATGCATTAAAAGTGGAGGCATATTTACATTTGTTTTGCATTTGTTATTTAAGAGGTCAGACTATGCAAAGTGCATCCACTTTTGTCAATAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA SEQUENCE_INCLUDED_REGION=36,231 P3_COMMENT=CL is CCTATAATTCAATAAAAAGTCAGCA P3_COMMENT=CR is AAAGTGGATGCACTTTGCAT P3_COMMENT=CM is TAACATGTCCAAGGGCATGT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.427417 PRIMER_LEFT_0_PENALTY=0.339170 PRIMER_RIGHT_0_PENALTY=0.088247 PRIMER_INTERNAL_0_PENALTY=0.184188 PRIMER_LEFT_0_SEQUENCE=GCAATAACATGTCCAAGGGC PRIMER_RIGHT_0_SEQUENCE=TTTTGCATGGTCCAGAACAA PRIMER_INTERNAL_0_SEQUENCE=AACACAAATATGCCCATGCA PRIMER_LEFT_0=76,20 PRIMER_RIGHT_0=175,20 PRIMER_INTERNAL_0=124,20 PRIMER_LEFT_0_TM=60.339 PRIMER_RIGHT_0_TM=60.088 PRIMER_INTERNAL_0_TM=59.816 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=0.693006 PRIMER_LEFT_1_PENALTY=0.604759 PRIMER_RIGHT_1_PENALTY=0.088247 PRIMER_INTERNAL_1_PENALTY=0.184188 PRIMER_LEFT_1_SEQUENCE=CAATAACATGTCCAAGGGCA PRIMER_RIGHT_1_SEQUENCE=TTTTGCATGGTCCAGAACAA PRIMER_INTERNAL_1_SEQUENCE=AACACAAATATGCCCATGCA PRIMER_LEFT_1=77,20 PRIMER_RIGHT_1=175,20 PRIMER_INTERNAL_1=124,20 PRIMER_LEFT_1_TM=59.395 PRIMER_RIGHT_1_TM=60.088 PRIMER_INTERNAL_1_TM=59.816 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=11.2000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=0.693006 PRIMER_LEFT_2_PENALTY=0.604759 PRIMER_RIGHT_2_PENALTY=0.088247 PRIMER_INTERNAL_2_PENALTY=0.184188 PRIMER_LEFT_2_SEQUENCE=CAATAACATGTCCAAGGGCA PRIMER_RIGHT_2_SEQUENCE=TTTTTGCATGGTCCAGAACA PRIMER_INTERNAL_2_SEQUENCE=AACACAAATATGCCCATGCA PRIMER_LEFT_2=77,20 PRIMER_RIGHT_2=176,20 PRIMER_INTERNAL_2=124,20 PRIMER_LEFT_2_TM=59.395 PRIMER_RIGHT_2_TM=60.088 PRIMER_INTERNAL_2_TM=59.816 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=11.2000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=0.777578 PRIMER_LEFT_3_PENALTY=0.339170 PRIMER_RIGHT_3_PENALTY=0.438408 PRIMER_INTERNAL_3_PENALTY=5.031983 PRIMER_LEFT_3_SEQUENCE=GCAATAACATGTCCAAGGGC PRIMER_RIGHT_3_SEQUENCE=TTTGCTAAAACATGCATGGG PRIMER_INTERNAL_3_SEQUENCE=CAAATGTGGATTTTAATGAAACACA PRIMER_LEFT_3=76,20 PRIMER_RIGHT_3=155,20 PRIMER_INTERNAL_3=105,25 PRIMER_LEFT_3_TM=60.339 PRIMER_RIGHT_3_TM=59.562 PRIMER_INTERNAL_3_TM=60.032 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=28.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=9.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=1.013002 PRIMER_LEFT_4_PENALTY=0.924755 PRIMER_RIGHT_4_PENALTY=0.088247 PRIMER_INTERNAL_4_PENALTY=0.184188 PRIMER_LEFT_4_SEQUENCE=TCCAAGGGCATGTTATCTCA PRIMER_RIGHT_4_SEQUENCE=TTTTGCATGGTCCAGAACAA PRIMER_INTERNAL_4_SEQUENCE=AACACAAATATGCCCATGCA PRIMER_LEFT_4=87,20 PRIMER_RIGHT_4=175,20 PRIMER_INTERNAL_4=124,20 PRIMER_LEFT_4_TM=59.075 PRIMER_RIGHT_4_TM=60.088 PRIMER_INTERNAL_4_TM=59.816 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH1200 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAATGTTAACATGCTGTGACCAGTAAACCAAAGATGCAGTGATTGCTAGTAGGCTCCATCGTCGGCCCCTTGCAGGTGGTGGGGACATATTCAGGGGTGGTGGATATCAGTGAGCACAGCCCCTGACTGGTGGCTGCCCATGCAGAGTGTGGGGGTGGGGAGTAGATGGGGGGCTTTTCCTAATGCATCTAGCACCCAGTGTCTCTGGCTTCCTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC SEQUENCE_INCLUDED_REGION=36,218 P3_COMMENT=CL is TGCTGTGACCAGTAAACCAA P3_COMMENT=CR is TAGGAAGCCAGAGACACTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.103650 PRIMER_LEFT_0_PENALTY=0.000037 PRIMER_RIGHT_0_PENALTY=0.103613 PRIMER_INTERNAL_0_PENALTY=0.049859 PRIMER_LEFT_0_SEQUENCE=GCTAGTAGGCTCCATCGTCG PRIMER_RIGHT_0_SEQUENCE=GGGGCTGTGCTCACTGATAT PRIMER_INTERNAL_0_SEQUENCE=GACATATTCAGGGGTGGTGG PRIMER_LEFT_0=82,20 PRIMER_RIGHT_0=160,20 PRIMER_INTERNAL_0=121,20 PRIMER_LEFT_0_TM=60.000 PRIMER_RIGHT_0_TM=60.104 PRIMER_INTERNAL_0_TM=60.050 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=10.1000 PRIMER_RIGHT_0_END_STABILITY=5.5000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=79 PRIMER_PAIR_1_PENALTY=0.146679 PRIMER_LEFT_1_PENALTY=0.049859 PRIMER_RIGHT_1_PENALTY=0.096820 PRIMER_INTERNAL_1_PENALTY=0.822509 PRIMER_LEFT_1_SEQUENCE=ACATATTCAGGGGTGGTGGA PRIMER_RIGHT_1_SEQUENCE=GAAAAGCCCCCCATCTACTC PRIMER_INTERNAL_1_SEQUENCE=TATCAGTGAGCACAGCCCCT PRIMER_LEFT_1=122,20 PRIMER_RIGHT_1=216,20 PRIMER_INTERNAL_1=142,20 PRIMER_LEFT_1_TM=60.050 PRIMER_RIGHT_1_TM=59.903 PRIMER_INTERNAL_1_TM=60.823 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=5.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=95 PRIMER_PAIR_2_PENALTY=0.146679 PRIMER_LEFT_2_PENALTY=0.049859 PRIMER_RIGHT_2_PENALTY=0.096820 PRIMER_INTERNAL_2_PENALTY=0.103613 PRIMER_LEFT_2_SEQUENCE=GACATATTCAGGGGTGGTGG PRIMER_RIGHT_2_SEQUENCE=GAAAAGCCCCCCATCTACTC PRIMER_INTERNAL_2_SEQUENCE=ATATCAGTGAGCACAGCCCC PRIMER_LEFT_2=121,20 PRIMER_RIGHT_2=216,20 PRIMER_INTERNAL_2=141,20 PRIMER_LEFT_2_TM=60.050 PRIMER_RIGHT_2_TM=59.903 PRIMER_INTERNAL_2_TM=60.104 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=5.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=0.146679 PRIMER_LEFT_3_PENALTY=0.049859 PRIMER_RIGHT_3_PENALTY=0.096820 PRIMER_INTERNAL_3_PENALTY=0.103613 PRIMER_LEFT_3_SEQUENCE=GGACATATTCAGGGGTGGTG PRIMER_RIGHT_3_SEQUENCE=GAAAAGCCCCCCATCTACTC PRIMER_INTERNAL_3_SEQUENCE=ATATCAGTGAGCACAGCCCC PRIMER_LEFT_3=120,20 PRIMER_RIGHT_3=216,20 PRIMER_INTERNAL_3=141,20 PRIMER_LEFT_3_TM=60.050 PRIMER_RIGHT_3_TM=59.903 PRIMER_INTERNAL_3_TM=60.104 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=5.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=0.200433 PRIMER_LEFT_4_PENALTY=0.103613 PRIMER_RIGHT_4_PENALTY=0.096820 PRIMER_INTERNAL_4_PENALTY=2.027014 PRIMER_LEFT_4_SEQUENCE=ATATCAGTGAGCACAGCCCC PRIMER_RIGHT_4_SEQUENCE=GAAAAGCCCCCCATCTACTC PRIMER_INTERNAL_4_SEQUENCE=CTGCCCATGCAGAGTGTG PRIMER_LEFT_4=141,20 PRIMER_RIGHT_4=216,20 PRIMER_INTERNAL_4=171,18 PRIMER_LEFT_4_TM=60.104 PRIMER_RIGHT_4_TM=59.903 PRIMER_INTERNAL_4_TM=59.973 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=12.4000 PRIMER_RIGHT_4_END_STABILITY=5.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH1203 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGTCCACATCCACTGCTTACTTACAAAAGAAAGTACAAACTTAGAGAAGGCCTTCCATGGTTTGGTTGCTACTGACTTTGTTAGCCTTACTGCCCACTATGCATTGGAACATTCCCATATTCCAACTAAGCAGGAGTGTTCACAATAAACAACATAGGCTCTTTATTCTCCTTCTTTCATTAATTTTCTTTCACGTTATTCCCTCACCCTGAACGCCCTTCTTCCTTCGTAGTGACATTTTAAAATCCACTTTACACATTCGGACCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTC SEQUENCE_INCLUDED_REGION=36,268 P3_COMMENT=CL is TCCATGGTTTGGTTGCTACT P3_COMMENT=CR is GGTCCGAATGTGTAAAGTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.094669 PRIMER_LEFT_0_PENALTY=0.073866 PRIMER_RIGHT_0_PENALTY=0.020803 PRIMER_INTERNAL_0_PENALTY=1.561873 PRIMER_LEFT_0_SEQUENCE=AGAGAAGGCCTTCCATGGTT PRIMER_RIGHT_0_SEQUENCE=ATGGGAATGTTCCAATGCAT PRIMER_INTERNAL_0_SEQUENCE=TTTGTTAGCCTTACTGCCCA PRIMER_LEFT_0=79,20 PRIMER_RIGHT_0=154,20 PRIMER_INTERNAL_0=113,20 PRIMER_LEFT_0_TM=60.074 PRIMER_RIGHT_0_TM=60.021 PRIMER_INTERNAL_0_TM=58.438 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=10.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=0.328864 PRIMER_LEFT_1_PENALTY=0.073866 PRIMER_RIGHT_1_PENALTY=0.254998 PRIMER_INTERNAL_1_PENALTY=1.561873 PRIMER_LEFT_1_SEQUENCE=AGAGAAGGCCTTCCATGGTT PRIMER_RIGHT_1_SEQUENCE=TGGGAATGTTCCAATGCATA PRIMER_INTERNAL_1_SEQUENCE=TTTGTTAGCCTTACTGCCCA PRIMER_LEFT_1=79,20 PRIMER_RIGHT_1=153,20 PRIMER_INTERNAL_1=113,20 PRIMER_LEFT_1_TM=60.074 PRIMER_RIGHT_1_TM=59.745 PRIMER_INTERNAL_1_TM=58.438 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=10.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=0.328864 PRIMER_LEFT_2_PENALTY=0.073866 PRIMER_RIGHT_2_PENALTY=0.254998 PRIMER_INTERNAL_2_PENALTY=1.561873 PRIMER_LEFT_2_SEQUENCE=AGAGAAGGCCTTCCATGGTT PRIMER_RIGHT_2_SEQUENCE=TATGGGAATGTTCCAATGCA PRIMER_INTERNAL_2_SEQUENCE=TTTGTTAGCCTTACTGCCCA PRIMER_LEFT_2=79,20 PRIMER_RIGHT_2=155,20 PRIMER_INTERNAL_2=113,20 PRIMER_LEFT_2_TM=60.074 PRIMER_RIGHT_2_TM=59.745 PRIMER_INTERNAL_2_TM=58.438 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=10.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=8.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=0.463701 PRIMER_LEFT_3_PENALTY=0.442898 PRIMER_RIGHT_3_PENALTY=0.020803 PRIMER_INTERNAL_3_PENALTY=1.561873 PRIMER_LEFT_3_SEQUENCE=GAGAAGGCCTTCCATGGTTT PRIMER_RIGHT_3_SEQUENCE=ATGGGAATGTTCCAATGCAT PRIMER_INTERNAL_3_SEQUENCE=TTTGTTAGCCTTACTGCCCA PRIMER_LEFT_3=80,20 PRIMER_RIGHT_3=154,20 PRIMER_INTERNAL_3=113,20 PRIMER_LEFT_3_TM=60.443 PRIMER_RIGHT_3_TM=60.021 PRIMER_INTERNAL_3_TM=58.438 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=10.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=8.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=0.697896 PRIMER_LEFT_4_PENALTY=0.442898 PRIMER_RIGHT_4_PENALTY=0.254998 PRIMER_INTERNAL_4_PENALTY=1.561873 PRIMER_LEFT_4_SEQUENCE=GAGAAGGCCTTCCATGGTTT PRIMER_RIGHT_4_SEQUENCE=TATGGGAATGTTCCAATGCA PRIMER_INTERNAL_4_SEQUENCE=TTTGTTAGCCTTACTGCCCA PRIMER_LEFT_4=80,20 PRIMER_RIGHT_4=155,20 PRIMER_INTERNAL_4=113,20 PRIMER_LEFT_4_TM=60.443 PRIMER_RIGHT_4_TM=59.745 PRIMER_INTERNAL_4_TM=58.438 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=10.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH1207 SEQUENCE_TEMPLATE=TNGCATTGCCTNCAGGTCGACTCTAGAGGATCCCGATGTGCTGCAAGGCGATTAAGTTGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTNCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG SEQUENCE_INCLUDED_REGION=34,24 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH1208 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTGCCTTTGAGATTTGTNCAAGTTATTGCTCATTATCTAGTTTGTNTCTTTTTATTGCCCTTGTCTTCTGTTTCATGGGTGTGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTC SEQUENCE_INCLUDED_REGION=35,81 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH1214 SEQUENCE_TEMPLATE=NTGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCTTCTTTGAATGTGCATGTGTGTGTGTGTGTCCTGGATGGGATCCTACTGCTATCTCTTCTTTTTCTCACCCAGTGACATGCCTTTCTATGCAGATCCATCTGGATCTNNTNTAGTTTTGCTCACTGCTGCGTAGCTTTCATGGTATGAATGCCAGAAGCCATTTAGCACCTCCCATAATGAGGGGATTTATGTTTTTNCCAGACCAGTGGTCTACAGATTGAGGGGTACNGAGNCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGA SEQUENCE_INCLUDED_REGION=38,226 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.228568 PRIMER_LEFT_0_PENALTY=0.202638 PRIMER_RIGHT_0_PENALTY=0.025930 PRIMER_INTERNAL_0_PENALTY=0.466916 PRIMER_LEFT_0_SEQUENCE=GTTTTGCTCACTGCTGCGTA PRIMER_RIGHT_0_SEQUENCE=AATCCCCTCATTATGGGAGG PRIMER_INTERNAL_0_SEQUENCE=ATGAATGCCAGAAGCCATTT PRIMER_LEFT_0=154,20 PRIMER_RIGHT_0=228,20 PRIMER_INTERNAL_0=185,20 PRIMER_LEFT_0_TM=60.203 PRIMER_RIGHT_0_TM=59.974 PRIMER_INTERNAL_0_TM=59.533 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=7.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.9000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=0.363749 PRIMER_LEFT_1_PENALTY=0.337818 PRIMER_RIGHT_1_PENALTY=0.025930 PRIMER_INTERNAL_1_PENALTY=0.466916 PRIMER_LEFT_1_SEQUENCE=TAGTTTTGCTCACTGCTGCG PRIMER_RIGHT_1_SEQUENCE=AATCCCCTCATTATGGGAGG PRIMER_INTERNAL_1_SEQUENCE=ATGAATGCCAGAAGCCATTT PRIMER_LEFT_1=152,20 PRIMER_RIGHT_1=228,20 PRIMER_INTERNAL_1=185,20 PRIMER_LEFT_1_TM=60.338 PRIMER_RIGHT_1_TM=59.974 PRIMER_INTERNAL_1_TM=59.533 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=10.2000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=0.702067 PRIMER_LEFT_2_PENALTY=0.624727 PRIMER_RIGHT_2_PENALTY=0.077340 PRIMER_INTERNAL_2_PENALTY=0.202638 PRIMER_LEFT_2_SEQUENCE=TGCCTTTCTATGCAGATCCA PRIMER_RIGHT_2_SEQUENCE=TATGGGAGGTGCTAAATGGC PRIMER_INTERNAL_2_SEQUENCE=GTTTTGCTCACTGCTGCGTA PRIMER_LEFT_2=119,20 PRIMER_RIGHT_2=217,20 PRIMER_INTERNAL_2=154,20 PRIMER_LEFT_2_TM=59.375 PRIMER_RIGHT_2_TM=59.923 PRIMER_INTERNAL_2_TM=60.203 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=9.6000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=5.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=0.712421 PRIMER_LEFT_3_PENALTY=0.087694 PRIMER_RIGHT_3_PENALTY=0.624727 PRIMER_INTERNAL_3_PENALTY=2.248781 PRIMER_LEFT_3_SEQUENCE=TGTGTGTGTGTGTGTCCTGG PRIMER_RIGHT_3_SEQUENCE=TGGATCTGCATAGAAAGGCA PRIMER_INTERNAL_3_SEQUENCE=TTCTTTTTCTCACCCAGTGACA PRIMER_LEFT_3=56,20 PRIMER_RIGHT_3=138,20 PRIMER_INTERNAL_3=97,22 PRIMER_LEFT_3_TM=60.088 PRIMER_RIGHT_3_TM=59.375 PRIMER_INTERNAL_3_TM=59.751 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=0.797136 PRIMER_LEFT_4_PENALTY=0.376364 PRIMER_RIGHT_4_PENALTY=0.420772 PRIMER_INTERNAL_4_PENALTY=0.120594 PRIMER_LEFT_4_SEQUENCE=TGTGCATGTGTGTGTGTGTG PRIMER_RIGHT_4_SEQUENCE=AGAAAGGCATGTCACTGGGT PRIMER_INTERNAL_4_SEQUENCE=TCCTGGATGGGATCCTACTG PRIMER_LEFT_4=50,20 PRIMER_RIGHT_4=127,20 PRIMER_INTERNAL_4=70,20 PRIMER_LEFT_4_TM=59.624 PRIMER_RIGHT_4_TM=59.579 PRIMER_INTERNAL_4_TM=59.879 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=78 = SEQUENCE_ID=MH1215 SEQUENCE_TEMPLATE=TTGCANNGCCTNCAGGTCGACTCTAGAGGATCCCCCTAGGCAGAGNNNTATCTCATTTTCTTTCATGGTTTAGGTNAAGTNACGTTAACTGCAGGCAGANGGCTAGTTTATAGTTTGAACAGACTCAAAATTNNTTTANACAGGACAAAGAAACAACAGAAGTACATATTATAAANCTTTTAAACTNTTCCCTAATATAACACTTCNTTTCCACTTGATACTGAAAATTTTTAAANTTCTCAACCTTAATTCAATTTACATCTATTTAAAAACATTATATAANACATTCTATATTANCAATGAATTGATTTGACTGTTTTC SEQUENCE_INCLUDED_REGION=35,286 P3_COMMENT=CL is CTCATTTTCTTTCATGGTTTAGG P3_COMMENT=CR is AAAACAGTCAAATCAATTCATTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=10.675442 PRIMER_LEFT_0_PENALTY=5.264677 PRIMER_RIGHT_0_PENALTY=5.410764 PRIMER_INTERNAL_0_PENALTY=8.349211 PRIMER_LEFT_0_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGG PRIMER_RIGHT_0_SEQUENCE=CTTCTGTTGTTTCTTTGTCCTGT PRIMER_INTERNAL_0_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA PRIMER_LEFT_0=50,24 PRIMER_RIGHT_0=161,23 PRIMER_INTERNAL_0=100,27 PRIMER_LEFT_0_TM=58.735 PRIMER_RIGHT_0_TM=57.589 PRIMER_INTERNAL_0_TM=58.651 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=39.130 PRIMER_INTERNAL_0_GC_PERCENT=37.037 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=1.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.5000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=112 PRIMER_PAIR_1_PENALTY=10.675442 PRIMER_LEFT_1_PENALTY=5.264677 PRIMER_RIGHT_1_PENALTY=5.410764 PRIMER_INTERNAL_1_PENALTY=8.349211 PRIMER_LEFT_1_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGG PRIMER_RIGHT_1_SEQUENCE=ACTTCTGTTGTTTCTTTGTCCTG PRIMER_INTERNAL_1_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA PRIMER_LEFT_1=50,24 PRIMER_RIGHT_1=162,23 PRIMER_INTERNAL_1=100,27 PRIMER_LEFT_1_TM=58.735 PRIMER_RIGHT_1_TM=57.589 PRIMER_INTERNAL_1_TM=58.651 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=39.130 PRIMER_INTERNAL_1_GC_PERCENT=37.037 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.5000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=113 PRIMER_PAIR_2_PENALTY=10.831436 PRIMER_LEFT_2_PENALTY=5.264677 PRIMER_RIGHT_2_PENALTY=5.566758 PRIMER_INTERNAL_2_PENALTY=8.349211 PRIMER_LEFT_2_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGG PRIMER_RIGHT_2_SEQUENCE=ACTTCTGTTGTTTCTTTGTCCTGT PRIMER_INTERNAL_2_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA PRIMER_LEFT_2=50,24 PRIMER_RIGHT_2=162,24 PRIMER_INTERNAL_2=100,27 PRIMER_LEFT_2_TM=58.735 PRIMER_RIGHT_2_TM=58.433 PRIMER_INTERNAL_2_TM=58.651 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=37.500 PRIMER_INTERNAL_2_GC_PERCENT=37.037 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.5000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=113 PRIMER_PAIR_3_PENALTY=10.939152 PRIMER_LEFT_3_PENALTY=5.528388 PRIMER_RIGHT_3_PENALTY=5.410764 PRIMER_INTERNAL_3_PENALTY=8.349211 PRIMER_LEFT_3_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGGT PRIMER_RIGHT_3_SEQUENCE=CTTCTGTTGTTTCTTTGTCCTGT PRIMER_INTERNAL_3_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA PRIMER_LEFT_3=50,25 PRIMER_RIGHT_3=161,23 PRIMER_INTERNAL_3=100,27 PRIMER_LEFT_3_TM=59.472 PRIMER_RIGHT_3_TM=57.589 PRIMER_INTERNAL_3_TM=58.651 PRIMER_LEFT_3_GC_PERCENT=32.000 PRIMER_RIGHT_3_GC_PERCENT=39.130 PRIMER_INTERNAL_3_GC_PERCENT=37.037 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=1.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=112 PRIMER_PAIR_4_PENALTY=10.939152 PRIMER_LEFT_4_PENALTY=5.528388 PRIMER_RIGHT_4_PENALTY=5.410764 PRIMER_INTERNAL_4_PENALTY=8.349211 PRIMER_LEFT_4_SEQUENCE=TCTCATTTTCTTTCATGGTTTAGGT PRIMER_RIGHT_4_SEQUENCE=ACTTCTGTTGTTTCTTTGTCCTG PRIMER_INTERNAL_4_SEQUENCE=GGCTAGTTTATAGTTTGAACAGACTCA PRIMER_LEFT_4=50,25 PRIMER_RIGHT_4=162,23 PRIMER_INTERNAL_4=100,27 PRIMER_LEFT_4_TM=59.472 PRIMER_RIGHT_4_TM=57.589 PRIMER_INTERNAL_4_TM=58.651 PRIMER_LEFT_4_GC_PERCENT=32.000 PRIMER_RIGHT_4_GC_PERCENT=39.130 PRIMER_INTERNAL_4_GC_PERCENT=37.037 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=113 = SEQUENCE_ID=MH1216 SEQUENCE_TEMPLATE=GCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCAGGGTNNTGACCTGTGGAGTCTCAACCCTAACAGCACATTAGCACCACTTGGGGAGCTTCTGAAAAATACTGGTACCCGGGGCCTTAGCATAGGTATTGTTCAAAACCTCTCAAGGTGATTTAAATGAGTAACAAGGGTTGAAAAACCNTGATTTTGGCAGCACAAACCAAAAGAGCAGGCAGGGCCGNGAGAGGGAAGTCAGTGGTACCAGAAGGTAGATGGGCTCCCTTGCAGGCTCCTTGTTCTCCTGCCATCACCAGTAGAACCTTCTGGCTGACAGACCAGGGACAAGTAGACTGGGTTCAAAGTG SEQUENCE_INCLUDED_REGION=32,314 P3_COMMENT=CL is TGACCTGTGGAGTCTCAACC P3_COMMENT=CR is TTGAACCCAGTCTACTTGTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.160863 PRIMER_LEFT_0_PENALTY=0.027122 PRIMER_RIGHT_0_PENALTY=0.133741 PRIMER_INTERNAL_0_PENALTY=0.979027 PRIMER_LEFT_0_SEQUENCE=CAGCACAAACCAAAAGAGCA PRIMER_RIGHT_0_SEQUENCE=GAGAACAAGGAGCCTGCAAG PRIMER_INTERNAL_0_SEQUENCE=CCAGAAGGTAGATGGGCTCC PRIMER_LEFT_0=193,20 PRIMER_RIGHT_0=282,20 PRIMER_INTERNAL_0=243,20 PRIMER_LEFT_0_TM=60.027 PRIMER_RIGHT_0_TM=60.134 PRIMER_INTERNAL_0_TM=60.979 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=90 PRIMER_PAIR_1_PENALTY=0.224025 PRIMER_LEFT_1_PENALTY=0.027122 PRIMER_RIGHT_1_PENALTY=0.196903 PRIMER_INTERNAL_1_PENALTY=0.455083 PRIMER_LEFT_1_SEQUENCE=CAGCACAAACCAAAAGAGCA PRIMER_RIGHT_1_SEQUENCE=GATGGCAGGAGAACAAGGAG PRIMER_INTERNAL_1_SEQUENCE=AAGGTAGATGGGCTCCCTTG PRIMER_LEFT_1=193,20 PRIMER_RIGHT_1=290,20 PRIMER_INTERNAL_1=247,20 PRIMER_LEFT_1_TM=60.027 PRIMER_RIGHT_1_TM=59.803 PRIMER_INTERNAL_1_TM=60.455 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=98 PRIMER_PAIR_2_PENALTY=0.683515 PRIMER_LEFT_2_PENALTY=0.027122 PRIMER_RIGHT_2_PENALTY=0.656393 PRIMER_INTERNAL_2_PENALTY=0.455083 PRIMER_LEFT_2_SEQUENCE=CAGCACAAACCAAAAGAGCA PRIMER_RIGHT_2_SEQUENCE=GTGATGGCAGGAGAACAAGG PRIMER_INTERNAL_2_SEQUENCE=AAGGTAGATGGGCTCCCTTG PRIMER_LEFT_2=193,20 PRIMER_RIGHT_2=292,20 PRIMER_INTERNAL_2=247,20 PRIMER_LEFT_2_TM=60.027 PRIMER_RIGHT_2_TM=60.656 PRIMER_INTERNAL_2_TM=60.455 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=0.778219 PRIMER_LEFT_3_PENALTY=0.466041 PRIMER_RIGHT_3_PENALTY=0.312177 PRIMER_INTERNAL_3_PENALTY=0.366450 PRIMER_LEFT_3_SEQUENCE=GACCTGTGGAGTCTCAACCC PRIMER_RIGHT_3_SEQUENCE=ATACCTATGCTAAGGCCCCG PRIMER_INTERNAL_3_SEQUENCE=CACTTGGGGAGCTTCTGAAA PRIMER_LEFT_3=43,20 PRIMER_RIGHT_3=131,20 PRIMER_INTERNAL_3=80,20 PRIMER_LEFT_3_TM=59.534 PRIMER_RIGHT_3_TM=60.312 PRIMER_INTERNAL_3_TM=60.366 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=12.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=89 PRIMER_PAIR_4_PENALTY=0.887023 PRIMER_LEFT_4_PENALTY=0.455083 PRIMER_RIGHT_4_PENALTY=0.431941 PRIMER_INTERNAL_4_PENALTY=0.119809 PRIMER_LEFT_4_SEQUENCE=AAGGTAGATGGGCTCCCTTG PRIMER_RIGHT_4_SEQUENCE=CCCAGTCTACTTGTCCCTGG PRIMER_INTERNAL_4_SEQUENCE=GTTCTCCTGCCATCACCAGT PRIMER_LEFT_4=247,20 PRIMER_RIGHT_4=336,20 PRIMER_INTERNAL_4=277,20 PRIMER_LEFT_4_TM=60.455 PRIMER_RIGHT_4_TM=59.568 PRIMER_INTERNAL_4_TM=60.120 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=90 = SEQUENCE_ID=MH1217 SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGGAAGACCAAAAAGACAGACCTCCCTTTCTTACTGAGAAGAGCGTAGTCAGACATGTCAAGTCTGGCACGCTGCTGCAGAAGGCGTGATTCATCTTAAGATCAAAAATTATTTCATCTTGCTTTCTTNNTCATAAGGGTACCGNGCTCGAATTCGTAATNATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATNCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGC SEQUENCE_INCLUDED_REGION=37,137 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.405698 PRIMER_LEFT_0_PENALTY=1.035219 PRIMER_RIGHT_0_PENALTY=0.370479 PRIMER_INTERNAL_0_PENALTY=0.318581 PRIMER_LEFT_0_SEQUENCE=GACCAAAAAGACAGACCTCCC PRIMER_RIGHT_0_SEQUENCE=AGATGAATCACGCCTTCTGC PRIMER_INTERNAL_0_SEQUENCE=GACATGTCAAGTCTGGCACG PRIMER_LEFT_0=43,21 PRIMER_RIGHT_0=133,20 PRIMER_INTERNAL_0=89,20 PRIMER_LEFT_0_TM=59.965 PRIMER_RIGHT_0_TM=60.370 PRIMER_INTERNAL_0_TM=60.319 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=91 PRIMER_PAIR_1_PENALTY=1.673804 PRIMER_LEFT_1_PENALTY=1.303324 PRIMER_RIGHT_1_PENALTY=0.370479 PRIMER_INTERNAL_1_PENALTY=0.318581 PRIMER_LEFT_1_SEQUENCE=TGGGAAGACCAAAAAGACAGA PRIMER_RIGHT_1_SEQUENCE=AGATGAATCACGCCTTCTGC PRIMER_INTERNAL_1_SEQUENCE=GACATGTCAAGTCTGGCACG PRIMER_LEFT_1=37,21 PRIMER_RIGHT_1=133,20 PRIMER_INTERNAL_1=89,20 PRIMER_LEFT_1_TM=59.697 PRIMER_RIGHT_1_TM=60.370 PRIMER_INTERNAL_1_TM=60.319 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=97 PRIMER_PAIR_2_PENALTY=1.768499 PRIMER_LEFT_2_PENALTY=1.398020 PRIMER_RIGHT_2_PENALTY=0.370479 PRIMER_INTERNAL_2_PENALTY=0.318581 PRIMER_LEFT_2_SEQUENCE=CCAAAAAGACAGACCTCCCTT PRIMER_RIGHT_2_SEQUENCE=AGATGAATCACGCCTTCTGC PRIMER_INTERNAL_2_SEQUENCE=GACATGTCAAGTCTGGCACG PRIMER_LEFT_2=45,21 PRIMER_RIGHT_2=133,20 PRIMER_INTERNAL_2=89,20 PRIMER_LEFT_2_TM=59.602 PRIMER_RIGHT_2_TM=60.370 PRIMER_INTERNAL_2_TM=60.319 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=89 PRIMER_PAIR_3_PENALTY=2.142615 PRIMER_LEFT_3_PENALTY=1.772136 PRIMER_RIGHT_3_PENALTY=0.370479 PRIMER_INTERNAL_3_PENALTY=0.318581 PRIMER_LEFT_3_SEQUENCE=CCAAAAAGACAGACCTCCCT PRIMER_RIGHT_3_SEQUENCE=AGATGAATCACGCCTTCTGC PRIMER_INTERNAL_3_SEQUENCE=GACATGTCAAGTCTGGCACG PRIMER_LEFT_3=45,20 PRIMER_RIGHT_3=133,20 PRIMER_INTERNAL_3=89,20 PRIMER_LEFT_3_TM=58.228 PRIMER_RIGHT_3_TM=60.370 PRIMER_INTERNAL_3_TM=60.319 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=89 PRIMER_PAIR_4_PENALTY=2.269568 PRIMER_LEFT_4_PENALTY=1.899089 PRIMER_RIGHT_4_PENALTY=0.370479 PRIMER_INTERNAL_4_PENALTY=0.318581 PRIMER_LEFT_4_SEQUENCE=ACCAAAAAGACAGACCTCCCT PRIMER_RIGHT_4_SEQUENCE=AGATGAATCACGCCTTCTGC PRIMER_INTERNAL_4_SEQUENCE=GACATGTCAAGTCTGGCACG PRIMER_LEFT_4=44,21 PRIMER_RIGHT_4=133,20 PRIMER_INTERNAL_4=89,20 PRIMER_LEFT_4_TM=59.101 PRIMER_RIGHT_4_TM=60.370 PRIMER_INTERNAL_4_TM=60.319 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=90 = SEQUENCE_ID=MH1218 SEQUENCE_TEMPLATE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGGACTACAGGGATCTCTTAAGCNCGGGAGGNGAGGNNAACGTGGGCTGTGATCGCACACTTCCACTCCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTNGGGAAACCTGTCGTGCCAGCTGCATTAATG SEQUENCE_INCLUDED_REGION=33,71 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH1221 SEQUENCE_TEMPLATE=TGCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTAAATGTNNCCTCTTCAGAAAAGCCCTCTCTGACCATCTGCTCAGAAGCAGTCCCATCCAGTGACTCTCCTTTGCTGTTATCACCACTTGAAGTTTGCAATTAGTGCATTTCTATGTGTACCTCCTGCTTTCCCCATTAGAATGTGTCTTAGTTCATGTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGNNNGCTAACTCACATTAATTGCGTTGCGCTCACTGC SEQUENCE_INCLUDED_REGION=37,164 P3_COMMENT=CL is CCTCTTCAGAAAAGCCCTCT P3_COMMENT=CR is ATTCTAATGGGGAAAGCAGG P3_COMMENT=CM is TGACCATCTGCTCAGAAGCA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.230886 PRIMER_LEFT_0_PENALTY=0.119809 PRIMER_RIGHT_0_PENALTY=0.111077 PRIMER_INTERNAL_0_PENALTY=2.132661 PRIMER_LEFT_0_SEQUENCE=AAGCAGTCCCATCCAGTGAC PRIMER_RIGHT_0_SEQUENCE=GGGAAAGCAGGAGGTACACA PRIMER_INTERNAL_0_SEQUENCE=TCACCACTTGAAGTTTGCAA PRIMER_LEFT_0=84,20 PRIMER_RIGHT_0=172,20 PRIMER_INTERNAL_0=119,20 PRIMER_LEFT_0_TM=60.120 PRIMER_RIGHT_0_TM=60.111 PRIMER_INTERNAL_0_TM=57.867 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=6.1000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=89 PRIMER_PAIR_1_PENALTY=0.371834 PRIMER_LEFT_1_PENALTY=0.260757 PRIMER_RIGHT_1_PENALTY=0.111077 PRIMER_INTERNAL_1_PENALTY=2.132661 PRIMER_LEFT_1_SEQUENCE=AGAAGCAGTCCCATCCAGTG PRIMER_RIGHT_1_SEQUENCE=GGGAAAGCAGGAGGTACACA PRIMER_INTERNAL_1_SEQUENCE=TCACCACTTGAAGTTTGCAA PRIMER_LEFT_1=82,20 PRIMER_RIGHT_1=172,20 PRIMER_INTERNAL_1=119,20 PRIMER_LEFT_1_TM=60.261 PRIMER_RIGHT_1_TM=60.111 PRIMER_INTERNAL_1_TM=57.867 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=0.371834 PRIMER_LEFT_2_PENALTY=0.260757 PRIMER_RIGHT_2_PENALTY=0.111077 PRIMER_INTERNAL_2_PENALTY=2.132661 PRIMER_LEFT_2_SEQUENCE=CAGAAGCAGTCCCATCCAGT PRIMER_RIGHT_2_SEQUENCE=GGGAAAGCAGGAGGTACACA PRIMER_INTERNAL_2_SEQUENCE=TCACCACTTGAAGTTTGCAA PRIMER_LEFT_2=81,20 PRIMER_RIGHT_2=172,20 PRIMER_INTERNAL_2=119,20 PRIMER_LEFT_2_TM=60.261 PRIMER_RIGHT_2_TM=60.111 PRIMER_INTERNAL_2_TM=57.867 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=92 PRIMER_PAIR_3_PENALTY=0.397701 PRIMER_LEFT_3_PENALTY=0.286624 PRIMER_RIGHT_3_PENALTY=0.111077 PRIMER_INTERNAL_3_PENALTY=2.132661 PRIMER_LEFT_3_SEQUENCE=AGCAGTCCCATCCAGTGACT PRIMER_RIGHT_3_SEQUENCE=GGGAAAGCAGGAGGTACACA PRIMER_INTERNAL_3_SEQUENCE=TCACCACTTGAAGTTTGCAA PRIMER_LEFT_3=85,20 PRIMER_RIGHT_3=172,20 PRIMER_INTERNAL_3=119,20 PRIMER_LEFT_3_TM=59.713 PRIMER_RIGHT_3_TM=60.111 PRIMER_INTERNAL_3_TM=57.867 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=88 PRIMER_PAIR_4_PENALTY=0.447343 PRIMER_LEFT_4_PENALTY=0.119809 PRIMER_RIGHT_4_PENALTY=0.327533 PRIMER_INTERNAL_4_PENALTY=2.132661 PRIMER_LEFT_4_SEQUENCE=AAGCAGTCCCATCCAGTGAC PRIMER_RIGHT_4_SEQUENCE=ATGGGGAAAGCAGGAGGTAC PRIMER_INTERNAL_4_SEQUENCE=TCACCACTTGAAGTTTGCAA PRIMER_LEFT_4=84,20 PRIMER_RIGHT_4=175,20 PRIMER_INTERNAL_4=119,20 PRIMER_LEFT_4_TM=60.120 PRIMER_RIGHT_4_TM=60.328 PRIMER_INTERNAL_4_TM=57.867 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=6.1000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=92 = SEQUENCE_ID=MH1225 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAATAGAAAGCATTGTCTATCTGTAACCTAACCTAGTNCCTCTAACACTTCCTTGCACTTGTTGTTTACAAGGCTGCTTCCCCATTAGACTACGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATNGTTATCCGCTCACANTTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTNCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC SEQUENCE_INCLUDED_REGION=36,97 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=8.426139 PRIMER_LEFT_0_PENALTY=7.827404 PRIMER_RIGHT_0_PENALTY=0.598735 PRIMER_INTERNAL_0_PENALTY=2.671989 PRIMER_LEFT_0_SEQUENCE=GCATTGTCTATCTGTAACCTAACCT PRIMER_RIGHT_0_SEQUENCE=GTCTAATGGGGAAGCAGCCT PRIMER_INTERNAL_0_SEQUENCE=CACTTCCTTGCACTTGTTGTTT PRIMER_LEFT_0=46,25 PRIMER_RIGHT_0=126,20 PRIMER_INTERNAL_0=82,22 PRIMER_LEFT_0_TM=57.173 PRIMER_RIGHT_0_TM=60.599 PRIMER_INTERNAL_0_TM=59.328 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=8.426139 PRIMER_LEFT_1_PENALTY=7.827404 PRIMER_RIGHT_1_PENALTY=0.598735 PRIMER_INTERNAL_1_PENALTY=2.671989 PRIMER_LEFT_1_SEQUENCE=GCATTGTCTATCTGTAACCTAACCT PRIMER_RIGHT_1_SEQUENCE=AGTCTAATGGGGAAGCAGCC PRIMER_INTERNAL_1_SEQUENCE=CACTTCCTTGCACTTGTTGTTT PRIMER_LEFT_1=46,25 PRIMER_RIGHT_1=127,20 PRIMER_INTERNAL_1=82,22 PRIMER_LEFT_1_TM=57.173 PRIMER_RIGHT_1_TM=60.599 PRIMER_INTERNAL_1_TM=59.328 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=8.426139 PRIMER_LEFT_2_PENALTY=7.827404 PRIMER_RIGHT_2_PENALTY=0.598735 PRIMER_INTERNAL_2_PENALTY=2.671989 PRIMER_LEFT_2_SEQUENCE=AGCATTGTCTATCTGTAACCTAACC PRIMER_RIGHT_2_SEQUENCE=GTCTAATGGGGAAGCAGCCT PRIMER_INTERNAL_2_SEQUENCE=CACTTCCTTGCACTTGTTGTTT PRIMER_LEFT_2=45,25 PRIMER_RIGHT_2=126,20 PRIMER_INTERNAL_2=82,22 PRIMER_LEFT_2_TM=57.173 PRIMER_RIGHT_2_TM=60.599 PRIMER_INTERNAL_2_TM=59.328 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=82 PRIMER_PAIR_3_PENALTY=8.426139 PRIMER_LEFT_3_PENALTY=7.827404 PRIMER_RIGHT_3_PENALTY=0.598735 PRIMER_INTERNAL_3_PENALTY=2.671989 PRIMER_LEFT_3_SEQUENCE=AGCATTGTCTATCTGTAACCTAACC PRIMER_RIGHT_3_SEQUENCE=AGTCTAATGGGGAAGCAGCC PRIMER_INTERNAL_3_SEQUENCE=CACTTCCTTGCACTTGTTGTTT PRIMER_LEFT_3=45,25 PRIMER_RIGHT_3=127,20 PRIMER_INTERNAL_3=82,22 PRIMER_LEFT_3_TM=57.173 PRIMER_RIGHT_3_TM=60.599 PRIMER_INTERNAL_3_TM=59.328 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=8.814430 PRIMER_LEFT_4_PENALTY=7.827404 PRIMER_RIGHT_4_PENALTY=0.987026 PRIMER_INTERNAL_4_PENALTY=3.333513 PRIMER_LEFT_4_SEQUENCE=AGCATTGTCTATCTGTAACCTAACC PRIMER_RIGHT_4_SEQUENCE=GGGGAAGCAGCCTTGTAAAC PRIMER_INTERNAL_4_SEQUENCE=CCTCTAACACTTCCTTGCACTTG PRIMER_LEFT_4=45,25 PRIMER_RIGHT_4=119,20 PRIMER_INTERNAL_4=75,23 PRIMER_LEFT_4_TM=57.173 PRIMER_RIGHT_4_TM=60.987 PRIMER_INTERNAL_4_TM=60.334 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=47.826 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH1227 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTGATAATTCACATACCATAAAATCCAACCATGGAAGGCACACAGTTCAGCAATTTTNAGTCTAGTCACAGAGTNGTGCGATCACCACTACAGTCTAATTTCAGATGTTTTCATTACCCCTAAAAGAAATCTTGTACCCATTAGCAATTATTCCTCATTCCTGCCCTCACCCCCAGGCCCTACTCTTTATCGCTATAGATTTGCCCACTTGACATATCATACACATGGAGCCATACATATGTGTGCCCTTCATGATTGGCTTCTTTCACTGAGAATAATGTTTTCAAGGT SEQUENCE_INCLUDED_REGION=36,291 P3_COMMENT=CL is CCATAAAATCCAACCATGGA P3_COMMENT=CR is TCATGAAGGGCACACATATG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.188598 PRIMER_LEFT_0_PENALTY=0.562302 PRIMER_RIGHT_0_PENALTY=0.626296 PRIMER_INTERNAL_0_PENALTY=1.249969 PRIMER_LEFT_0_SEQUENCE=CCCCAGGCCCTACTCTTTAT PRIMER_RIGHT_0_SEQUENCE=CAATCATGAAGGGCACACAT PRIMER_INTERNAL_0_SEQUENCE=CGCTATAGATTTGCCCACTTG PRIMER_LEFT_0=207,20 PRIMER_RIGHT_0=294,20 PRIMER_INTERNAL_0=227,21 PRIMER_LEFT_0_TM=59.438 PRIMER_RIGHT_0_TM=59.374 PRIMER_INTERNAL_0_TM=59.750 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.2000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 PRIMER_PAIR_1_PENALTY=1.436802 PRIMER_LEFT_1_PENALTY=0.562302 PRIMER_RIGHT_1_PENALTY=0.874500 PRIMER_INTERNAL_1_PENALTY=1.249969 PRIMER_LEFT_1_SEQUENCE=CCCCAGGCCCTACTCTTTAT PRIMER_RIGHT_1_SEQUENCE=AAGAAGCCAATCATGAAGGG PRIMER_INTERNAL_1_SEQUENCE=CGCTATAGATTTGCCCACTTG PRIMER_LEFT_1=207,20 PRIMER_RIGHT_1=301,20 PRIMER_INTERNAL_1=227,21 PRIMER_LEFT_1_TM=59.438 PRIMER_RIGHT_1_TM=59.126 PRIMER_INTERNAL_1_TM=59.750 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.2000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=95 PRIMER_PAIR_2_PENALTY=1.653378 PRIMER_LEFT_2_PENALTY=0.562302 PRIMER_RIGHT_2_PENALTY=1.091076 PRIMER_INTERNAL_2_PENALTY=1.249969 PRIMER_LEFT_2_SEQUENCE=CCCCAGGCCCTACTCTTTAT PRIMER_RIGHT_2_SEQUENCE=CCAATCATGAAGGGCACAC PRIMER_INTERNAL_2_SEQUENCE=CGCTATAGATTTGCCCACTTG PRIMER_LEFT_2=207,20 PRIMER_RIGHT_2=295,19 PRIMER_INTERNAL_2=227,21 PRIMER_LEFT_2_TM=59.438 PRIMER_RIGHT_2_TM=59.909 PRIMER_INTERNAL_2_TM=59.750 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=52.632 PRIMER_INTERNAL_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.2000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=89 PRIMER_PAIR_3_PENALTY=1.674482 PRIMER_LEFT_3_PENALTY=1.048185 PRIMER_RIGHT_3_PENALTY=0.626296 PRIMER_INTERNAL_3_PENALTY=2.170825 PRIMER_LEFT_3_SEQUENCE=CAGGCCCTACTCTTTATCGC PRIMER_RIGHT_3_SEQUENCE=CAATCATGAAGGGCACACAT PRIMER_INTERNAL_3_SEQUENCE=GATTTGCCCACTTGACATATCA PRIMER_LEFT_3=210,20 PRIMER_RIGHT_3=294,20 PRIMER_INTERNAL_3=234,22 PRIMER_LEFT_3_TM=58.952 PRIMER_RIGHT_3_TM=59.374 PRIMER_INTERNAL_3_TM=59.829 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=9.8000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=85 PRIMER_PAIR_4_PENALTY=1.741440 PRIMER_LEFT_4_PENALTY=1.115144 PRIMER_RIGHT_4_PENALTY=0.626296 PRIMER_INTERNAL_4_PENALTY=2.170825 PRIMER_LEFT_4_SEQUENCE=CAGGCCCTACTCTTTATCGCT PRIMER_RIGHT_4_SEQUENCE=CAATCATGAAGGGCACACAT PRIMER_INTERNAL_4_SEQUENCE=GATTTGCCCACTTGACATATCA PRIMER_LEFT_4=210,21 PRIMER_RIGHT_4=294,20 PRIMER_INTERNAL_4=234,22 PRIMER_LEFT_4_TM=59.885 PRIMER_RIGHT_4_TM=59.374 PRIMER_INTERNAL_4_TM=59.829 PRIMER_LEFT_4_GC_PERCENT=52.381 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=9.9000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=85 = SEQUENCE_ID=MH1228 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTTGCATGAACAAAATTGGAAAGGAATGTATTCTGTTTNTCTGAATTTCCTTAATTTGCTTCTTTCCTTAATGTNCAAATTTGAGATGTGTGATATCAACTTGGGAGACATATATCAACGATAATATTTTATAAGAGCATTTTCTAAAATAATACAAAAATGCAGAATAATAGGATGGATTTGTTGGAGAAATAATGGGCTTTGAATCTGGATATTTCTGGGTTCAGATCTCAGCCCTGCCACTTACTCATTACATGACCTTGCATGTGTCACTTCCCCTCTCAAAGTC SEQUENCE_INCLUDED_REGION=36,288 P3_COMMENT=CL is TGAGATGTGTGATATCAACTTGG P3_COMMENT=CR is GAGAGGGGAAGTGACACATG P3_COMMENT=CM is ACAAAAATGCAGAATAATAGGATGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.586514 PRIMER_LEFT_0_PENALTY=1.466705 PRIMER_RIGHT_0_PENALTY=0.119809 PRIMER_INTERNAL_0_PENALTY=0.012470 PRIMER_LEFT_0_SEQUENCE=TGGAGAAATAATGGGCTTTGA PRIMER_RIGHT_0_SEQUENCE=AGAGGGGAAGTGACACATGC PRIMER_INTERNAL_0_SEQUENCE=CTCAGCCCTGCCACTTACTC PRIMER_LEFT_0=219,21 PRIMER_RIGHT_0=316,20 PRIMER_INTERNAL_0=264,20 PRIMER_LEFT_0_TM=59.533 PRIMER_RIGHT_0_TM=60.120 PRIMER_INTERNAL_0_TM=60.012 PRIMER_LEFT_0_GC_PERCENT=38.095 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=8.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=98 PRIMER_PAIR_1_PENALTY=1.666329 PRIMER_LEFT_1_PENALTY=1.546520 PRIMER_RIGHT_1_PENALTY=0.119809 PRIMER_INTERNAL_1_PENALTY=0.012470 PRIMER_LEFT_1_SEQUENCE=AATGGGCTTTGAATCTGGAT PRIMER_RIGHT_1_SEQUENCE=AGAGGGGAAGTGACACATGC PRIMER_INTERNAL_1_SEQUENCE=CTCAGCCCTGCCACTTACTC PRIMER_LEFT_1=228,20 PRIMER_RIGHT_1=316,20 PRIMER_INTERNAL_1=264,20 PRIMER_LEFT_1_TM=58.453 PRIMER_RIGHT_1_TM=60.120 PRIMER_INTERNAL_1_TM=60.012 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.1000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=89 PRIMER_PAIR_2_PENALTY=1.959681 PRIMER_LEFT_2_PENALTY=1.839872 PRIMER_RIGHT_2_PENALTY=0.119809 PRIMER_INTERNAL_2_PENALTY=0.012470 PRIMER_LEFT_2_SEQUENCE=TAATGGGCTTTGAATCTGGA PRIMER_RIGHT_2_SEQUENCE=AGAGGGGAAGTGACACATGC PRIMER_INTERNAL_2_SEQUENCE=CTCAGCCCTGCCACTTACTC PRIMER_LEFT_2=227,20 PRIMER_RIGHT_2=316,20 PRIMER_INTERNAL_2=264,20 PRIMER_LEFT_2_TM=58.160 PRIMER_RIGHT_2_TM=60.120 PRIMER_INTERNAL_2_TM=60.012 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=8.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=2.055020 PRIMER_LEFT_3_PENALTY=1.935211 PRIMER_RIGHT_3_PENALTY=0.119809 PRIMER_INTERNAL_3_PENALTY=0.012470 PRIMER_LEFT_3_SEQUENCE=TTGGAGAAATAATGGGCTTTG PRIMER_RIGHT_3_SEQUENCE=AGAGGGGAAGTGACACATGC PRIMER_INTERNAL_3_SEQUENCE=CTCAGCCCTGCCACTTACTC PRIMER_LEFT_3=218,21 PRIMER_RIGHT_3=316,20 PRIMER_INTERNAL_3=264,20 PRIMER_LEFT_3_TM=59.065 PRIMER_RIGHT_3_TM=60.120 PRIMER_INTERNAL_3_TM=60.012 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=8.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=2.276288 PRIMER_LEFT_4_PENALTY=2.156479 PRIMER_RIGHT_4_PENALTY=0.119809 PRIMER_INTERNAL_4_PENALTY=0.012470 PRIMER_LEFT_4_SEQUENCE=GTTGGAGAAATAATGGGCTTTG PRIMER_RIGHT_4_SEQUENCE=AGAGGGGAAGTGACACATGC PRIMER_INTERNAL_4_SEQUENCE=CTCAGCCCTGCCACTTACTC PRIMER_LEFT_4=217,22 PRIMER_RIGHT_4=316,20 PRIMER_INTERNAL_4=264,20 PRIMER_LEFT_4_TM=59.844 PRIMER_RIGHT_4_TM=60.120 PRIMER_INTERNAL_4_TM=60.012 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=8.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH1230 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCCCTCCACCTCTGGCCCACACACCAAATCACACTCTCTACATTTTCTGATTTCTGTATTGAGACCAAGTTTTGATTTATATTTCTGAGGGGTCAATCTGCAATTGACCNACGTGCCAAATCTGTAGACTATATCCAGTTTTCAAACTTATTTCCACATCCTTCAAAACAACTGCTCACACCATTGCTCATCTCCCTACTTCTGCATTTCAACACTACTCAGGGAGACGTGGGACAGNANGNTCTGTGGCACAGCACAGGCCCACAGTGGGCTCATCTAGTGCACTGTGTCTGGACCCTGCTCTGC SEQUENCE_INCLUDED_REGION=36,307 P3_COMMENT=CL is CCACACACCAAATCACACTC P3_COMMENT=CR is ACACAGTGCACTAGATGAGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.426859 PRIMER_LEFT_0_PENALTY=0.111814 PRIMER_RIGHT_0_PENALTY=0.315045 PRIMER_INTERNAL_0_PENALTY=0.668065 PRIMER_LEFT_0_SEQUENCE=TACTCAGGGAGACGTGGGAC PRIMER_RIGHT_0_SEQUENCE=GAGCAGGGTCCAGACACAGT PRIMER_INTERNAL_0_SEQUENCE=CCCACAGTGGGCTCATCTAG PRIMER_LEFT_0=252,20 PRIMER_RIGHT_0=339,20 PRIMER_INTERNAL_0=297,20 PRIMER_LEFT_0_TM=60.112 PRIMER_RIGHT_0_TM=60.315 PRIMER_INTERNAL_0_TM=60.668 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=9.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=9.1000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 PRIMER_PAIR_1_PENALTY=0.567294 PRIMER_LEFT_1_PENALTY=0.252249 PRIMER_RIGHT_1_PENALTY=0.315045 PRIMER_INTERNAL_1_PENALTY=0.668065 PRIMER_LEFT_1_SEQUENCE=CTACTCAGGGAGACGTGGGA PRIMER_RIGHT_1_SEQUENCE=GAGCAGGGTCCAGACACAGT PRIMER_INTERNAL_1_SEQUENCE=CCCACAGTGGGCTCATCTAG PRIMER_LEFT_1=251,20 PRIMER_RIGHT_1=339,20 PRIMER_INTERNAL_1=297,20 PRIMER_LEFT_1_TM=60.252 PRIMER_RIGHT_1_TM=60.315 PRIMER_INTERNAL_1_TM=60.668 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=9.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=89 PRIMER_PAIR_2_PENALTY=0.569545 PRIMER_LEFT_2_PENALTY=0.111814 PRIMER_RIGHT_2_PENALTY=0.457731 PRIMER_INTERNAL_2_PENALTY=0.008216 PRIMER_LEFT_2_SEQUENCE=TACTCAGGGAGACGTGGGAC PRIMER_RIGHT_2_SEQUENCE=AGAGCAGGGTCCAGACACAG PRIMER_INTERNAL_2_SEQUENCE=CAGTGGGCTCATCTAGTGCA PRIMER_LEFT_2=252,20 PRIMER_RIGHT_2=340,20 PRIMER_INTERNAL_2=301,20 PRIMER_LEFT_2_TM=60.112 PRIMER_RIGHT_2_TM=60.458 PRIMER_INTERNAL_2_TM=60.008 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=9.1000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=89 PRIMER_PAIR_3_PENALTY=0.709980 PRIMER_LEFT_3_PENALTY=0.252249 PRIMER_RIGHT_3_PENALTY=0.457731 PRIMER_INTERNAL_3_PENALTY=0.008216 PRIMER_LEFT_3_SEQUENCE=CTACTCAGGGAGACGTGGGA PRIMER_RIGHT_3_SEQUENCE=AGAGCAGGGTCCAGACACAG PRIMER_INTERNAL_3_SEQUENCE=CAGTGGGCTCATCTAGTGCA PRIMER_LEFT_3=251,20 PRIMER_RIGHT_3=340,20 PRIMER_INTERNAL_3=301,20 PRIMER_LEFT_3_TM=60.252 PRIMER_RIGHT_3_TM=60.458 PRIMER_INTERNAL_3_TM=60.008 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=0.789588 PRIMER_LEFT_4_PENALTY=0.716637 PRIMER_RIGHT_4_PENALTY=0.072951 PRIMER_INTERNAL_4_PENALTY=5.746125 PRIMER_LEFT_4_SEQUENCE=CCCACACACCAAATCACACT PRIMER_RIGHT_4_SEQUENCE=ATTGCAGATTGACCCCTCAG PRIMER_INTERNAL_4_SEQUENCE=TTTCTGTATTGAGACCAAGTTTTGA PRIMER_LEFT_4=52,20 PRIMER_RIGHT_4=140,20 PRIMER_INTERNAL_4=87,25 PRIMER_LEFT_4_TM=59.283 PRIMER_RIGHT_4_TM=60.073 PRIMER_INTERNAL_4_TM=59.254 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=32.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.1000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH1231 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTTTCCCCTCCTACGTNAGAGTNNATACCCAAGGAAGCGGAAGTAGAAGATGACTCTTCAAGAGANNTATCCCCTGCTGTTACATGTATACATACANGATACATACATATGGAATGCATGGGAAAGGAAGGCATTAGAATTCCACCTGAACTTTCTGTGCTTAAAAGATTAGAGGAGACAGTGTACAAAAAGAGAGATCATTGATTCTCCGCCCACTGCCTTGACTCTATGTGTGTTTTCTATTTTGATACTGTTGCTCCTCTTATTCACATGCTGTTTAAAATATACAGGGGTACCGAGNCTCGAATTCGTAATCATGGTCATAGCTGT SEQUENCE_INCLUDED_REGION=36,291 P3_COMMENT=CL is CAAGGAAGCGGAAGTAGAAG P3_COMMENT=CR is CATAGAGTCAAGGCAGTGGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.111146 PRIMER_LEFT_0_PENALTY=0.298125 PRIMER_RIGHT_0_PENALTY=0.813021 PRIMER_INTERNAL_0_PENALTY=5.287628 PRIMER_LEFT_0_SEQUENCE=TACCCAAGGAAGCGGAAGTA PRIMER_RIGHT_0_SEQUENCE=TTTCCCATGCATTCCATATG PRIMER_INTERNAL_0_SEQUENCE=TCCCCTGCTGTTACATGTATACATA PRIMER_LEFT_0=62,20 PRIMER_RIGHT_0=161,20 PRIMER_INTERNAL_0=106,25 PRIMER_LEFT_0_TM=59.702 PRIMER_RIGHT_0_TM=59.187 PRIMER_INTERNAL_0_TM=59.712 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=5.7000 PRIMER_RIGHT_0_END_STABILITY=5.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=1.212472 PRIMER_LEFT_1_PENALTY=0.552282 PRIMER_RIGHT_1_PENALTY=0.660189 PRIMER_INTERNAL_1_PENALTY=5.287628 PRIMER_LEFT_1_SEQUENCE=CCAAGGAAGCGGAAGTAGAA PRIMER_RIGHT_1_SEQUENCE=CCTTTCCCATGCATTCCATA PRIMER_INTERNAL_1_SEQUENCE=TCCCCTGCTGTTACATGTATACATA PRIMER_LEFT_1=65,20 PRIMER_RIGHT_1=163,20 PRIMER_INTERNAL_1=106,25 PRIMER_LEFT_1_TM=59.448 PRIMER_RIGHT_1_TM=60.660 PRIMER_INTERNAL_1_TM=59.712 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=8.00 PRIMER_LEFT_1_END_STABILITY=6.0000 PRIMER_RIGHT_1_END_STABILITY=7.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=1.328827 PRIMER_LEFT_2_PENALTY=0.298125 PRIMER_RIGHT_2_PENALTY=1.030702 PRIMER_INTERNAL_2_PENALTY=5.287628 PRIMER_LEFT_2_SEQUENCE=TACCCAAGGAAGCGGAAGTA PRIMER_RIGHT_2_SEQUENCE=TTTCCCATGCATTCCATATGT PRIMER_INTERNAL_2_SEQUENCE=TCCCCTGCTGTTACATGTATACATA PRIMER_LEFT_2=62,20 PRIMER_RIGHT_2=161,21 PRIMER_INTERNAL_2=106,25 PRIMER_LEFT_2_TM=59.702 PRIMER_RIGHT_2_TM=60.031 PRIMER_INTERNAL_2_TM=59.712 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=38.095 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=10.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=8.00 PRIMER_LEFT_2_END_STABILITY=5.7000 PRIMER_RIGHT_2_END_STABILITY=5.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=1.365304 PRIMER_LEFT_3_PENALTY=0.552282 PRIMER_RIGHT_3_PENALTY=0.813021 PRIMER_INTERNAL_3_PENALTY=5.287628 PRIMER_LEFT_3_SEQUENCE=CCAAGGAAGCGGAAGTAGAA PRIMER_RIGHT_3_SEQUENCE=TTTCCCATGCATTCCATATG PRIMER_INTERNAL_3_SEQUENCE=TCCCCTGCTGTTACATGTATACATA PRIMER_LEFT_3=65,20 PRIMER_RIGHT_3=161,20 PRIMER_INTERNAL_3=106,25 PRIMER_LEFT_3_TM=59.448 PRIMER_RIGHT_3_TM=59.187 PRIMER_INTERNAL_3_TM=59.712 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=10.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=8.00 PRIMER_LEFT_3_END_STABILITY=6.0000 PRIMER_RIGHT_3_END_STABILITY=5.8000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=1.392215 PRIMER_LEFT_4_PENALTY=0.038361 PRIMER_RIGHT_4_PENALTY=1.353854 PRIMER_INTERNAL_4_PENALTY=5.287628 PRIMER_LEFT_4_SEQUENCE=ATACCCAAGGAAGCGGAAGT PRIMER_RIGHT_4_SEQUENCE=TTCCCATGCATTCCATATGT PRIMER_INTERNAL_4_SEQUENCE=TCCCCTGCTGTTACATGTATACATA PRIMER_LEFT_4=61,20 PRIMER_RIGHT_4=160,20 PRIMER_INTERNAL_4=106,25 PRIMER_LEFT_4_TM=59.962 PRIMER_RIGHT_4_TM=58.646 PRIMER_INTERNAL_4_TM=59.712 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=10.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=8.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=5.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH1232 SEQUENCE_TEMPLATE=CTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCTACCCACCCCCTTCACCCCACCTGAATGAGATGATTTCTGAAGTCCTCAGCAGCCCTGATTCTAAGCCTCATAAGGAAGAGTAGGTGTTAATGGCATCCTAGGGCAATGGTAGGTGCCTGATGCAGATCTGCTGTGAGCCATGTGCTGGCATCACAGGGGTGGTTTATTAATTTCATTTATCATCTGGACAGCCCCTTCTTATAACGTACATCCTTGCCTCTTCTGAGGCGCTAAGATCCCCAAGGTGGCTCCTGTATCCAGAAACCCNTNGGCCTTCCCTCCCAGTTA SEQUENCE_INCLUDED_REGION=34,289 P3_COMMENT=CL is GATGATTTCTGAAGTCCTCAGC P3_COMMENT=CR is TTTCTGGATACAGGAGCCAC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.157984 PRIMER_LEFT_0_PENALTY=0.051674 PRIMER_RIGHT_0_PENALTY=0.106310 PRIMER_INTERNAL_0_PENALTY=0.305090 PRIMER_LEFT_0_SEQUENCE=TCCTAGGGCAATGGTAGGTG PRIMER_RIGHT_0_SEQUENCE=GGGGCTGTCCAGATGATAAA PRIMER_INTERNAL_0_SEQUENCE=GATGCAGATCTGCTGTGAGC PRIMER_LEFT_0=130,20 PRIMER_RIGHT_0=229,20 PRIMER_INTERNAL_0=153,20 PRIMER_LEFT_0_TM=59.948 PRIMER_RIGHT_0_TM=59.894 PRIMER_INTERNAL_0_TM=59.695 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=0.179262 PRIMER_LEFT_1_PENALTY=0.106310 PRIMER_RIGHT_1_PENALTY=0.072951 PRIMER_INTERNAL_1_PENALTY=0.056936 PRIMER_LEFT_1_SEQUENCE=TTTATCATCTGGACAGCCCC PRIMER_RIGHT_1_SEQUENCE=TTCTGGATACAGGAGCCACC PRIMER_INTERNAL_1_SEQUENCE=CATCCTTGCCTCTTCTGAGG PRIMER_LEFT_1=210,20 PRIMER_RIGHT_1=297,20 PRIMER_INTERNAL_1=243,20 PRIMER_LEFT_1_TM=59.894 PRIMER_RIGHT_1_TM=60.073 PRIMER_INTERNAL_1_TM=59.943 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=12.4000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=88 PRIMER_PAIR_2_PENALTY=0.209924 PRIMER_LEFT_2_PENALTY=0.103613 PRIMER_RIGHT_2_PENALTY=0.106310 PRIMER_INTERNAL_2_PENALTY=0.323721 PRIMER_LEFT_2_SEQUENCE=GGTAGGTGCCTGATGCAGAT PRIMER_RIGHT_2_SEQUENCE=GGGGCTGTCCAGATGATAAA PRIMER_INTERNAL_2_SEQUENCE=GCATCACAGGGGTGGTTTAT PRIMER_LEFT_2=142,20 PRIMER_RIGHT_2=229,20 PRIMER_INTERNAL_2=182,20 PRIMER_LEFT_2_TM=60.104 PRIMER_RIGHT_2_TM=59.894 PRIMER_INTERNAL_2_TM=59.676 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=6.2000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=88 PRIMER_PAIR_3_PENALTY=0.221430 PRIMER_LEFT_3_PENALTY=0.117817 PRIMER_RIGHT_3_PENALTY=0.103613 PRIMER_INTERNAL_3_PENALTY=0.077340 PRIMER_LEFT_3_SEQUENCE=CAGCAGCCCTGATTCTAAGC PRIMER_RIGHT_3_SEQUENCE=ATCTGCATCAGGCACCTACC PRIMER_INTERNAL_3_SEQUENCE=GTTAATGGCATCCTAGGGCA PRIMER_LEFT_3=81,20 PRIMER_RIGHT_3=161,20 PRIMER_INTERNAL_3=120,20 PRIMER_LEFT_3_TM=60.118 PRIMER_RIGHT_3_TM=60.104 PRIMER_INTERNAL_3_TM=59.923 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.5000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=81 PRIMER_PAIR_4_PENALTY=0.340133 PRIMER_LEFT_4_PENALTY=0.262792 PRIMER_RIGHT_4_PENALTY=0.077340 PRIMER_INTERNAL_4_PENALTY=0.117817 PRIMER_LEFT_4_SEQUENCE=CCCCACCTGAATGAGATGAT PRIMER_RIGHT_4_SEQUENCE=GCCCTAGGATGCCATTAACA PRIMER_INTERNAL_4_SEQUENCE=CAGCAGCCCTGATTCTAAGC PRIMER_LEFT_4=49,20 PRIMER_RIGHT_4=138,20 PRIMER_INTERNAL_4=81,20 PRIMER_LEFT_4_TM=59.737 PRIMER_RIGHT_4_TM=59.923 PRIMER_INTERNAL_4_TM=60.118 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.5000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=90 = SEQUENCE_ID=MH1233 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAATATTATAGAACATTATATTTGCATGTATCCAAGTNGCATTATTGAAAAACTCATTTGGATNAACATTGAGATTTTAGGAGTNNCTTTAAAAGAGTNNTGTACATAACTACATTTAAGAAACTCCCTCATGACAATTCCATGAGATAGTTACTATTATCCACATTTTGCAGATGAAGATACTGAGACTTAACAAAAATAAGTAAAATTGTCTACGGTTATGAGTNTAGCAAGATACCAGTGAAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTC SEQUENCE_INCLUDED_REGION=36,247 P3_COMMENT=CL is GAACATTATATTTGCATGTATCCA P3_COMMENT=CR is CCGTAGACAATTTTACTTATTTTTG P3_COMMENT=CM is GCATTATTGAAAAACTCATTTGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=10.747149 PRIMER_LEFT_0_PENALTY=4.460533 PRIMER_RIGHT_0_PENALTY=6.286617 PRIMER_INTERNAL_0_PENALTY=9.564502 PRIMER_LEFT_0_SEQUENCE=CATTTAAGAAACTCCCTCATGACA PRIMER_RIGHT_0_SEQUENCE=GTCTCAGTATCTTCATCTGCAAAA PRIMER_INTERNAL_0_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA PRIMER_LEFT_0=150,24 PRIMER_RIGHT_0=226,24 PRIMER_INTERNAL_0=176,27 PRIMER_LEFT_0_TM=59.539 PRIMER_RIGHT_0_TM=57.713 PRIMER_INTERNAL_0_TM=57.435 PRIMER_LEFT_0_GC_PERCENT=37.500 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_INTERNAL_0_GC_PERCENT=33.333 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=9.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=10.794372 PRIMER_LEFT_1_PENALTY=4.460533 PRIMER_RIGHT_1_PENALTY=6.333840 PRIMER_INTERNAL_1_PENALTY=9.564502 PRIMER_LEFT_1_SEQUENCE=CATTTAAGAAACTCCCTCATGACA PRIMER_RIGHT_1_SEQUENCE=AGTCTCAGTATCTTCATCTGCAAAA PRIMER_INTERNAL_1_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA PRIMER_LEFT_1=150,24 PRIMER_RIGHT_1=227,25 PRIMER_INTERNAL_1=176,27 PRIMER_LEFT_1_TM=59.539 PRIMER_RIGHT_1_TM=58.666 PRIMER_INTERNAL_1_TM=57.435 PRIMER_LEFT_1_GC_PERCENT=37.500 PRIMER_RIGHT_1_GC_PERCENT=36.000 PRIMER_INTERNAL_1_GC_PERCENT=33.333 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=9.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=10.794372 PRIMER_LEFT_2_PENALTY=4.460533 PRIMER_RIGHT_2_PENALTY=6.333840 PRIMER_INTERNAL_2_PENALTY=9.564502 PRIMER_LEFT_2_SEQUENCE=CATTTAAGAAACTCCCTCATGACA PRIMER_RIGHT_2_SEQUENCE=AAGTCTCAGTATCTTCATCTGCAAA PRIMER_INTERNAL_2_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA PRIMER_LEFT_2=150,24 PRIMER_RIGHT_2=228,25 PRIMER_INTERNAL_2=176,27 PRIMER_LEFT_2_TM=59.539 PRIMER_RIGHT_2_TM=58.666 PRIMER_INTERNAL_2_TM=57.435 PRIMER_LEFT_2_GC_PERCENT=37.500 PRIMER_RIGHT_2_GC_PERCENT=36.000 PRIMER_INTERNAL_2_GC_PERCENT=33.333 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=9.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=8.8000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=79 PRIMER_PAIR_3_PENALTY=11.114895 PRIMER_LEFT_3_PENALTY=4.460533 PRIMER_RIGHT_3_PENALTY=6.654362 PRIMER_INTERNAL_3_PENALTY=9.564502 PRIMER_LEFT_3_SEQUENCE=CATTTAAGAAACTCCCTCATGACA PRIMER_RIGHT_3_SEQUENCE=AGTCTCAGTATCTTCATCTGCAAA PRIMER_INTERNAL_3_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA PRIMER_LEFT_3=150,24 PRIMER_RIGHT_3=227,24 PRIMER_INTERNAL_3=176,27 PRIMER_LEFT_3_TM=59.539 PRIMER_RIGHT_3_TM=57.346 PRIMER_INTERNAL_3_TM=57.435 PRIMER_LEFT_3_GC_PERCENT=37.500 PRIMER_RIGHT_3_GC_PERCENT=37.500 PRIMER_INTERNAL_3_GC_PERCENT=33.333 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=9.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=8.8000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=78 PRIMER_PAIR_4_PENALTY=11.114895 PRIMER_LEFT_4_PENALTY=4.460533 PRIMER_RIGHT_4_PENALTY=6.654362 PRIMER_INTERNAL_4_PENALTY=9.564502 PRIMER_LEFT_4_SEQUENCE=CATTTAAGAAACTCCCTCATGACA PRIMER_RIGHT_4_SEQUENCE=AAGTCTCAGTATCTTCATCTGCAA PRIMER_INTERNAL_4_SEQUENCE=TCCATGAGATAGTTACTATTATCCACA PRIMER_LEFT_4=150,24 PRIMER_RIGHT_4=228,24 PRIMER_INTERNAL_4=176,27 PRIMER_LEFT_4_TM=59.539 PRIMER_RIGHT_4_TM=57.346 PRIMER_INTERNAL_4_TM=57.435 PRIMER_LEFT_4_GC_PERCENT=37.500 PRIMER_RIGHT_4_GC_PERCENT=37.500 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=9.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=8.8000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH1234 SEQUENCE_TEMPLATE=CTTGCANGCCTCCAGGTCGACTCTAGAGGATCCCCCTTTGTNGTNNCTCTTCTTTGTNGATCAGAAATTACAGCAGGAACTACTGCTACTGGGTNAGGAAACCTAAATGCAATGNGGATCACTGGATNCAGGGTNNCACTTTAGTCGCCAAAGACAAGGTGGNATGGNTACTATAATAGACATCAGAATCAAAGCAATAATCAGAATAGCCTAAGAGATGTATGGCATTGGCTAGTTGATCATAATGTCTCCACAAGTGAAGTAGNTNGGCAGTCAACAAAATTCTTACTTGATTTGTNTANGGGNAACAACTCCATATCAAGTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTG SEQUENCE_INCLUDED_REGION=35,291 P3_COMMENT=CL is GAAATTACAGCAGGAACTACTGC P3_COMMENT=CR is AAGTAAGAATTTTGTTGACTGCC P3_COMMENT=CM is CACTTTAGTCGCCAAAGACAA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.353299 PRIMER_LEFT_0_PENALTY=2.133445 PRIMER_RIGHT_0_PENALTY=0.219854 PRIMER_INTERNAL_0_PENALTY=5.342972 PRIMER_LEFT_0_SEQUENCE=AGTCGCCAAAGACAAGGTG PRIMER_RIGHT_0_SEQUENCE=TGATCAACTAGCCAATGCCA PRIMER_INTERNAL_0_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA PRIMER_LEFT_0=142,19 PRIMER_RIGHT_0=241,20 PRIMER_INTERNAL_0=180,25 PRIMER_LEFT_0_TM=58.867 PRIMER_RIGHT_0_TM=60.220 PRIMER_INTERNAL_0_TM=59.657 PRIMER_LEFT_0_GC_PERCENT=52.632 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=10.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=2.765222 PRIMER_LEFT_1_PENALTY=2.545368 PRIMER_RIGHT_1_PENALTY=0.219854 PRIMER_INTERNAL_1_PENALTY=5.342972 PRIMER_LEFT_1_SEQUENCE=AGTCGCCAAAGACAAGGTGG PRIMER_RIGHT_1_SEQUENCE=TGATCAACTAGCCAATGCCA PRIMER_INTERNAL_1_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA PRIMER_LEFT_1=142,20 PRIMER_RIGHT_1=241,20 PRIMER_INTERNAL_1=180,25 PRIMER_LEFT_1_TM=62.545 PRIMER_RIGHT_1_TM=60.220 PRIMER_INTERNAL_1_TM=59.657 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=32.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=2.872243 PRIMER_LEFT_2_PENALTY=2.652389 PRIMER_RIGHT_2_PENALTY=0.219854 PRIMER_INTERNAL_2_PENALTY=5.342972 PRIMER_LEFT_2_SEQUENCE=GTCGCCAAAGACAAGGTGG PRIMER_RIGHT_2_SEQUENCE=TGATCAACTAGCCAATGCCA PRIMER_INTERNAL_2_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA PRIMER_LEFT_2=143,19 PRIMER_RIGHT_2=241,20 PRIMER_INTERNAL_2=180,25 PRIMER_LEFT_2_TM=61.652 PRIMER_RIGHT_2_TM=60.220 PRIMER_INTERNAL_2_TM=59.657 PRIMER_LEFT_2_GC_PERCENT=57.895 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=32.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=2.881005 PRIMER_LEFT_3_PENALTY=1.076949 PRIMER_RIGHT_3_PENALTY=1.804056 PRIMER_INTERNAL_3_PENALTY=5.342972 PRIMER_LEFT_3_SEQUENCE=TAGTCGCCAAAGACAAGGTG PRIMER_RIGHT_3_SEQUENCE=TCAACTAGCCAATGCCATACA PRIMER_INTERNAL_3_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA PRIMER_LEFT_3=141,20 PRIMER_RIGHT_3=238,21 PRIMER_INTERNAL_3=180,25 PRIMER_LEFT_3_TM=58.923 PRIMER_RIGHT_3_TM=59.196 PRIMER_INTERNAL_3_TM=59.657 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_INTERNAL_3_GC_PERCENT=32.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=5.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=3.026281 PRIMER_LEFT_4_PENALTY=2.806427 PRIMER_RIGHT_4_PENALTY=0.219854 PRIMER_INTERNAL_4_PENALTY=5.342972 PRIMER_LEFT_4_SEQUENCE=TCGCCAAAGACAAGGTGG PRIMER_RIGHT_4_SEQUENCE=TGATCAACTAGCCAATGCCA PRIMER_INTERNAL_4_SEQUENCE=CATCAGAATCAAAGCAATAATCAGA PRIMER_LEFT_4=144,18 PRIMER_RIGHT_4=241,20 PRIMER_INTERNAL_4=180,25 PRIMER_LEFT_4_TM=60.806 PRIMER_RIGHT_4_TM=60.220 PRIMER_INTERNAL_4_TM=59.657 PRIMER_LEFT_4_GC_PERCENT=55.556 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=32.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=10.0000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH1236 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTACATGTNNNACAGGTCTTGTTTCAAAAACTTAATCCCCATGAAAGTTTCTGNTTGTACCCCAGATGGTAGTGTGTAACTTGCTTCTCCATCAGGGAAAGTCCTGCTGTCCATGTGAGGCTCTGGCTCTNCCATTTTCCACCCCTGGGCTGTGATCACAGCAGAGCAGGCTTCTAGAATNCATGTCCCCAGCACCCCATCCTTGCAGCAATGGCGAGGAGACAGCAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAC SEQUENCE_INCLUDED_REGION=36,230 P3_COMMENT=CL is TTGTTTCAAAAACTTAATCCCC P3_COMMENT=CR is GTCTCCTCGCCATTGCTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.706826 PRIMER_LEFT_0_PENALTY=0.196903 PRIMER_RIGHT_0_PENALTY=0.509923 PRIMER_INTERNAL_0_PENALTY=0.738752 PRIMER_LEFT_0_SEQUENCE=ACTTGCTTCTCCATCAGGGA PRIMER_RIGHT_0_SEQUENCE=TAGAAGCCTGCTCTGCTGTG PRIMER_INTERNAL_0_SEQUENCE=AAGTCCTGCTGTCCATGTGA PRIMER_LEFT_0=114,20 PRIMER_RIGHT_0=211,20 PRIMER_INTERNAL_0=134,20 PRIMER_LEFT_0_TM=59.803 PRIMER_RIGHT_0_TM=59.490 PRIMER_INTERNAL_0_TM=59.261 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=98 PRIMER_PAIR_1_PENALTY=0.840967 PRIMER_LEFT_1_PENALTY=0.331043 PRIMER_RIGHT_1_PENALTY=0.509923 PRIMER_INTERNAL_1_PENALTY=1.003769 PRIMER_LEFT_1_SEQUENCE=TGCTTCTCCATCAGGGAAAG PRIMER_RIGHT_1_SEQUENCE=TAGAAGCCTGCTCTGCTGTG PRIMER_INTERNAL_1_SEQUENCE=CTGCTGTCCATGTGAGGCT PRIMER_LEFT_1=117,20 PRIMER_RIGHT_1=211,20 PRIMER_INTERNAL_1=139,19 PRIMER_LEFT_1_TM=60.331 PRIMER_RIGHT_1_TM=59.490 PRIMER_INTERNAL_1_TM=59.996 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=7.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=95 PRIMER_PAIR_2_PENALTY=0.840967 PRIMER_LEFT_2_PENALTY=0.331043 PRIMER_RIGHT_2_PENALTY=0.509923 PRIMER_INTERNAL_2_PENALTY=1.003769 PRIMER_LEFT_2_SEQUENCE=CTTGCTTCTCCATCAGGGAA PRIMER_RIGHT_2_SEQUENCE=TAGAAGCCTGCTCTGCTGTG PRIMER_INTERNAL_2_SEQUENCE=CTGCTGTCCATGTGAGGCT PRIMER_LEFT_2=115,20 PRIMER_RIGHT_2=211,20 PRIMER_INTERNAL_2=139,19 PRIMER_LEFT_2_TM=60.331 PRIMER_RIGHT_2_TM=59.490 PRIMER_INTERNAL_2_TM=59.996 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=97 PRIMER_PAIR_3_PENALTY=0.861071 PRIMER_LEFT_3_PENALTY=0.351148 PRIMER_RIGHT_3_PENALTY=0.509923 PRIMER_INTERNAL_3_PENALTY=1.853206 PRIMER_LEFT_3_SEQUENCE=CTCCATCAGGGAAAGTCCTG PRIMER_RIGHT_3_SEQUENCE=TAGAAGCCTGCTCTGCTGTG PRIMER_INTERNAL_3_SEQUENCE=TGTCCATGTGAGGCTCTGG PRIMER_LEFT_3=122,20 PRIMER_RIGHT_3=211,20 PRIMER_INTERNAL_3=143,19 PRIMER_LEFT_3_TM=59.649 PRIMER_RIGHT_3_TM=59.490 PRIMER_INTERNAL_3_TM=60.853 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=57.895 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=0.895679 PRIMER_LEFT_4_PENALTY=0.385756 PRIMER_RIGHT_4_PENALTY=0.509923 PRIMER_INTERNAL_4_PENALTY=1.910613 PRIMER_LEFT_4_SEQUENCE=TCAGGGAAAGTCCTGCTGTC PRIMER_RIGHT_4_SEQUENCE=TAGAAGCCTGCTCTGCTGTG PRIMER_INTERNAL_4_SEQUENCE=CATGTGAGGCTCTGGCTCT PRIMER_LEFT_4=127,20 PRIMER_RIGHT_4=211,20 PRIMER_INTERNAL_4=147,19 PRIMER_LEFT_4_TM=60.386 PRIMER_RIGHT_4_TM=59.490 PRIMER_INTERNAL_4_TM=59.089 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=57.895 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=85 = SEQUENCE_ID=MH1237 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCTTTTCTTTCAGTNCTTCATTTCCANNATCGACTTGTGAGAAAAATGTCTTTGACACAGAAAGAAGTGCTTCTCTGGGCCACTGTNNNTCAAGGAAAAATCTAGAAAATCTGTTGTNTNATTTCAATAACATACAGTTTAATACCAAGAAAAACATATTTCTCGGGAACTCCTCCCTGCCCAGTNCCCTCTTCACCTAGTTAACTCCTACTCATGTCTATGGTGCTTAATGAACTGAAGGGCTCTGTNCATTTGAGGGNTGCCTGGGTNTGCATTTAGTGACCAGTATGTCTCCACAGTATCATGCACACGGA SEQUENCE_INCLUDED_REGION=36,312 P3_COMMENT=CL is GTGAGAAAAATGTCTTTGACACA P3_COMMENT=CR is GCCCTTCAGTTCATTAAGCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.900545 PRIMER_LEFT_0_PENALTY=2.013682 PRIMER_RIGHT_0_PENALTY=0.886863 PRIMER_INTERNAL_0_PENALTY=5.661030 PRIMER_LEFT_0_SEQUENCE=TGCTTAATGAACTGAAGGGCT PRIMER_RIGHT_0_SEQUENCE=TCCGTGTGCATGATACTGTG PRIMER_INTERNAL_0_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC PRIMER_LEFT_0=257,21 PRIMER_RIGHT_0=347,20 PRIMER_INTERNAL_0=304,24 PRIMER_LEFT_0_TM=58.986 PRIMER_RIGHT_0_TM=59.113 PRIMER_INTERNAL_0_TM=58.339 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=91 PRIMER_PAIR_1_PENALTY=2.915660 PRIMER_LEFT_1_PENALTY=2.028797 PRIMER_RIGHT_1_PENALTY=0.886863 PRIMER_INTERNAL_1_PENALTY=5.661030 PRIMER_LEFT_1_SEQUENCE=TGCTTAATGAACTGAAGGGC PRIMER_RIGHT_1_SEQUENCE=TCCGTGTGCATGATACTGTG PRIMER_INTERNAL_1_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC PRIMER_LEFT_1=257,20 PRIMER_RIGHT_1=347,20 PRIMER_INTERNAL_1=304,24 PRIMER_LEFT_1_TM=57.971 PRIMER_RIGHT_1_TM=59.113 PRIMER_INTERNAL_1_TM=58.339 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=10.9000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=3.037245 PRIMER_LEFT_2_PENALTY=2.150382 PRIMER_RIGHT_2_PENALTY=0.886863 PRIMER_INTERNAL_2_PENALTY=5.661030 PRIMER_LEFT_2_SEQUENCE=GTGCTTAATGAACTGAAGGGC PRIMER_RIGHT_2_SEQUENCE=TCCGTGTGCATGATACTGTG PRIMER_INTERNAL_2_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC PRIMER_LEFT_2=256,21 PRIMER_RIGHT_2=347,20 PRIMER_INTERNAL_2=304,24 PRIMER_LEFT_2_TM=58.850 PRIMER_RIGHT_2_TM=59.113 PRIMER_INTERNAL_2_TM=58.339 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=10.9000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=92 PRIMER_PAIR_3_PENALTY=3.097749 PRIMER_LEFT_3_PENALTY=2.210886 PRIMER_RIGHT_3_PENALTY=0.886863 PRIMER_INTERNAL_3_PENALTY=5.661030 PRIMER_LEFT_3_SEQUENCE=GTGCTTAATGAACTGAAGGGCT PRIMER_RIGHT_3_SEQUENCE=TCCGTGTGCATGATACTGTG PRIMER_INTERNAL_3_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC PRIMER_LEFT_3=256,22 PRIMER_RIGHT_3=347,20 PRIMER_INTERNAL_3=304,24 PRIMER_LEFT_3_TM=59.789 PRIMER_RIGHT_3_TM=59.113 PRIMER_INTERNAL_3_TM=58.339 PRIMER_LEFT_3_GC_PERCENT=45.455 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=92 PRIMER_PAIR_4_PENALTY=3.179952 PRIMER_LEFT_4_PENALTY=2.293089 PRIMER_RIGHT_4_PENALTY=0.886863 PRIMER_INTERNAL_4_PENALTY=5.661030 PRIMER_LEFT_4_SEQUENCE=GGTGCTTAATGAACTGAAGGG PRIMER_RIGHT_4_SEQUENCE=TCCGTGTGCATGATACTGTG PRIMER_INTERNAL_4_SEQUENCE=TGCATTTAGTGACCAGTATGTCTC PRIMER_LEFT_4=255,21 PRIMER_RIGHT_4=347,20 PRIMER_INTERNAL_4=304,24 PRIMER_LEFT_4_TM=58.707 PRIMER_RIGHT_4_TM=59.113 PRIMER_INTERNAL_4_TM=58.339 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=93 = SEQUENCE_ID=MH1238 SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGTTTGTNGTNTTGACTGTTTGTTTGTTTTTTTACTGCCTACAAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTT SEQUENCE_INCLUDED_REGION=35,44 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH1239 SEQUENCE_TEMPLATE=TTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTACAACTGTGAGCAGCAATGCCGTCTTTCAGTATAGCATGCTTCATATTTGCAGAGATCCGCATGCACATAATATTCTCACCATCACAACACAGTTAAGTATGCTTGCACACGTGTGTACACACACACACACACACACACACACACTGAGAAGGCAGTCAGTGACTACCTTCCATTTTCCAACCACAAACTGCTGTCAGAATGAATACTACGTTTTAGCTTTTCAAATAACTGGAAATAACATCAGATTAAGTAAAGAAGACAGGGTAC SEQUENCE_INCLUDED_REGION=34,271 P3_COMMENT=CL is GCAATGCCGTCTTTCAGTAT P3_COMMENT=CR is AAACGTAGTATTCATTCTGACAGC P3_COMMENT=CM is GCATGCTTCATATTTGCAGA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.228181 PRIMER_LEFT_0_PENALTY=0.207669 PRIMER_RIGHT_0_PENALTY=1.020512 PRIMER_INTERNAL_0_PENALTY=3.883941 PRIMER_LEFT_0_SEQUENCE=TATTTGCAGAGATCCGCATG PRIMER_RIGHT_0_SEQUENCE=TGTACACACGTGTGCAAGCA PRIMER_INTERNAL_0_SEQUENCE=TTCTCACCATCACAACACAGTT PRIMER_LEFT_0=81,20 PRIMER_RIGHT_0=156,20 PRIMER_INTERNAL_0=110,22 PRIMER_LEFT_0_TM=59.792 PRIMER_RIGHT_0_TM=61.021 PRIMER_INTERNAL_0_TM=58.116 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=10.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=7.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=1.228181 PRIMER_LEFT_1_PENALTY=0.207669 PRIMER_RIGHT_1_PENALTY=1.020512 PRIMER_INTERNAL_1_PENALTY=3.883941 PRIMER_LEFT_1_SEQUENCE=CATATTTGCAGAGATCCGCA PRIMER_RIGHT_1_SEQUENCE=TGTACACACGTGTGCAAGCA PRIMER_INTERNAL_1_SEQUENCE=TTCTCACCATCACAACACAGTT PRIMER_LEFT_1=79,20 PRIMER_RIGHT_1=156,20 PRIMER_INTERNAL_1=110,22 PRIMER_LEFT_1_TM=59.792 PRIMER_RIGHT_1_TM=61.021 PRIMER_INTERNAL_1_TM=58.116 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=10.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=11.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=1.286948 PRIMER_LEFT_2_PENALTY=1.020512 PRIMER_RIGHT_2_PENALTY=0.266437 PRIMER_INTERNAL_2_PENALTY=1.079874 PRIMER_LEFT_2_SEQUENCE=TGCTTGCACACGTGTGTACA PRIMER_RIGHT_2_SEQUENCE=CAGCAGTTTGTGGTTGGAAA PRIMER_INTERNAL_2_SEQUENCE=CACACACTGAGAAGGCAGTCA PRIMER_LEFT_2=137,20 PRIMER_RIGHT_2=230,20 PRIMER_INTERNAL_2=175,21 PRIMER_LEFT_2_TM=61.021 PRIMER_RIGHT_2_TM=59.734 PRIMER_INTERNAL_2_TM=60.080 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=10.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=8.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=5.4000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=94 PRIMER_PAIR_3_PENALTY=1.294259 PRIMER_LEFT_3_PENALTY=1.020512 PRIMER_RIGHT_3_PENALTY=0.273747 PRIMER_INTERNAL_3_PENALTY=1.079874 PRIMER_LEFT_3_SEQUENCE=TGCTTGCACACGTGTGTACA PRIMER_RIGHT_3_SEQUENCE=GACAGCAGTTTGTGGTTGGA PRIMER_INTERNAL_3_SEQUENCE=CACACACTGAGAAGGCAGTCA PRIMER_LEFT_3=137,20 PRIMER_RIGHT_3=232,20 PRIMER_INTERNAL_3=175,21 PRIMER_LEFT_3_TM=61.021 PRIMER_RIGHT_3_TM=59.726 PRIMER_INTERNAL_3_TM=60.080 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=10.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=8.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=5.4000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=96 PRIMER_PAIR_4_PENALTY=1.389613 PRIMER_LEFT_4_PENALTY=0.207669 PRIMER_RIGHT_4_PENALTY=1.181943 PRIMER_INTERNAL_4_PENALTY=3.883941 PRIMER_LEFT_4_SEQUENCE=TATTTGCAGAGATCCGCATG PRIMER_RIGHT_4_SEQUENCE=TGTGTGTGTACACACGTGTGC PRIMER_INTERNAL_4_SEQUENCE=TTCTCACCATCACAACACAGTT PRIMER_LEFT_4=81,20 PRIMER_RIGHT_4=162,21 PRIMER_INTERNAL_4=110,22 PRIMER_LEFT_4_TM=59.792 PRIMER_RIGHT_4_TM=60.182 PRIMER_INTERNAL_4_TM=58.116 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=12.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=8.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH124 SEQUENCE_TEMPLATE=AGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCGAGGCCAGGAGGGTAGGTTGATTTCAAAGCGGGCAGATGAATGTCTTATTTAGGAGTAGGATTGGGTTCACTCAAATATAAGTTTTATTTGAATTGCAAAAGGCGGCAGGTTAGATGCTTGTATACATTCTCATGTATTTNTNATTTTAAACTCTGCTGTGCCTTGGTTACATCTTTGACCTTCTTAAGAATGAGTGAAGACATTTGTTTCATTTAGCATTGGTTGAGCCTCAGATGGAGGTTGGATATTTGATTATGAAAGATGGAACGAAATGTTAG SEQUENCE_INCLUDED_REGION=36,282 P3_COMMENT=CL is CCAGGAGGGTAGGTTGATTT P3_COMMENT=CR is GAGGCTCAACCAATGCTAAA P3_COMMENT=CM is AAAGCGGGCAGATGAATGTC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.158903 PRIMER_LEFT_0_PENALTY=0.085951 PRIMER_RIGHT_0_PENALTY=0.072951 PRIMER_INTERNAL_0_PENALTY=5.711025 PRIMER_LEFT_0_SEQUENCE=AACTCTGCTGTGCCTTGGTT PRIMER_RIGHT_0_SEQUENCE=CCATCTGAGGCTCAACCAAT PRIMER_INTERNAL_0_SEQUENCE=GAATGAGTGAAGACATTTGTTTCA PRIMER_LEFT_0=188,20 PRIMER_RIGHT_0=276,20 PRIMER_INTERNAL_0=227,24 PRIMER_LEFT_0_TM=59.914 PRIMER_RIGHT_0_TM=60.073 PRIMER_INTERNAL_0_TM=58.289 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=33.333 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=89 PRIMER_PAIR_1_PENALTY=0.158903 PRIMER_LEFT_1_PENALTY=0.085951 PRIMER_RIGHT_1_PENALTY=0.072951 PRIMER_INTERNAL_1_PENALTY=5.711025 PRIMER_LEFT_1_SEQUENCE=AAACTCTGCTGTGCCTTGGT PRIMER_RIGHT_1_SEQUENCE=CCATCTGAGGCTCAACCAAT PRIMER_INTERNAL_1_SEQUENCE=GAATGAGTGAAGACATTTGTTTCA PRIMER_LEFT_1=187,20 PRIMER_RIGHT_1=276,20 PRIMER_INTERNAL_1=227,24 PRIMER_LEFT_1_TM=59.914 PRIMER_RIGHT_1_TM=60.073 PRIMER_INTERNAL_1_TM=58.289 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=33.333 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=8.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=90 PRIMER_PAIR_2_PENALTY=0.282855 PRIMER_LEFT_2_PENALTY=0.085951 PRIMER_RIGHT_2_PENALTY=0.196903 PRIMER_INTERNAL_2_PENALTY=4.212142 PRIMER_LEFT_2_SEQUENCE=AACTCTGCTGTGCCTTGGTT PRIMER_RIGHT_2_SEQUENCE=AACCTCCATCTGAGGCTCAA PRIMER_INTERNAL_2_SEQUENCE=GACATTTGTTTCATTTAGCATTGG PRIMER_LEFT_2=188,20 PRIMER_RIGHT_2=281,20 PRIMER_INTERNAL_2=238,24 PRIMER_LEFT_2_TM=59.914 PRIMER_RIGHT_2_TM=59.803 PRIMER_INTERNAL_2_TM=59.788 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=33.333 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=94 PRIMER_PAIR_3_PENALTY=0.282855 PRIMER_LEFT_3_PENALTY=0.085951 PRIMER_RIGHT_3_PENALTY=0.196903 PRIMER_INTERNAL_3_PENALTY=4.212142 PRIMER_LEFT_3_SEQUENCE=AAACTCTGCTGTGCCTTGGT PRIMER_RIGHT_3_SEQUENCE=AACCTCCATCTGAGGCTCAA PRIMER_INTERNAL_3_SEQUENCE=GACATTTGTTTCATTTAGCATTGG PRIMER_LEFT_3=187,20 PRIMER_RIGHT_3=281,20 PRIMER_INTERNAL_3=238,24 PRIMER_LEFT_3_TM=59.914 PRIMER_RIGHT_3_TM=59.803 PRIMER_INTERNAL_3_TM=59.788 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=33.333 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=95 PRIMER_PAIR_4_PENALTY=0.312095 PRIMER_LEFT_4_PENALTY=0.085951 PRIMER_RIGHT_4_PENALTY=0.226143 PRIMER_INTERNAL_4_PENALTY=5.711025 PRIMER_LEFT_4_SEQUENCE=AACTCTGCTGTGCCTTGGTT PRIMER_RIGHT_4_SEQUENCE=ATCTGAGGCTCAACCAATGC PRIMER_INTERNAL_4_SEQUENCE=GAATGAGTGAAGACATTTGTTTCA PRIMER_LEFT_4=188,20 PRIMER_RIGHT_4=274,20 PRIMER_INTERNAL_4=227,24 PRIMER_LEFT_4_TM=59.914 PRIMER_RIGHT_4_TM=60.226 PRIMER_INTERNAL_4_TM=58.289 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=8.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.4000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=7.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH1240 SEQUENCE_TEMPLATE=GNTNGCANNCCTCCAGGTCGACTCTAGAGGATCCCTGGCCTTGCAGACATNACCAGACGACACCCAAGAAAAATGGCAGCGCAGAGCCGTGAGCATCACAGGAGGTCTCAGGGNCACGCCAAGGCCGCCTCCCAGGANAAAGCAGAACACCGGAAAAGACCTTGTGAAGACTTNTTATCTTCCCCTCCAAATGGTGTCTCTTGCTTTGGCTCTTGTCGAGTTGGGGCTTGTTGGAAGACCAGGTGAAGGCCCAGGGTNNCGAGCTCGANTTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAANTTGTTATCCGNTCACAATTCCACACAACATACGNGCCGGAAGCATAAAGTGTAAAG SEQUENCE_INCLUDED_REGION=35,218 P3_COMMENT=CL is ACCAGACGACACCCAAGAAA P3_COMMENT=CR is GGCCTTCACCTGGTCTTCC P3_COMMENT=CM is GCAGAGCCGTGAGCATCA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.176548 PRIMER_LEFT_0_PENALTY=0.029253 PRIMER_RIGHT_0_PENALTY=0.147295 PRIMER_INTERNAL_0_PENALTY=0.667795 PRIMER_LEFT_0_SEQUENCE=ACACCCAAGAAAAATGGCAG PRIMER_RIGHT_0_SEQUENCE=TCTTTTCCGGTGTTCTGCTT PRIMER_INTERNAL_0_SEQUENCE=CATCACAGGAGGTCTCAGGG PRIMER_LEFT_0=59,20 PRIMER_RIGHT_0=158,20 PRIMER_INTERNAL_0=93,20 PRIMER_LEFT_0_TM=59.971 PRIMER_RIGHT_0_TM=59.853 PRIMER_INTERNAL_0_TM=60.668 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=0.384190 PRIMER_LEFT_1_PENALTY=0.005232 PRIMER_RIGHT_1_PENALTY=0.378958 PRIMER_INTERNAL_1_PENALTY=1.011075 PRIMER_LEFT_1_SEQUENCE=ACACCGGAAAAGACCTTGTG PRIMER_RIGHT_1_SEQUENCE=AACTCGACAAGAGCCAAAGC PRIMER_INTERNAL_1_SEQUENCE=CCCTCCAAATGGTGTCTCTT PRIMER_LEFT_1=146,20 PRIMER_RIGHT_1=221,20 PRIMER_INTERNAL_1=182,20 PRIMER_LEFT_1_TM=60.005 PRIMER_RIGHT_1_TM=59.621 PRIMER_INTERNAL_1_TM=58.989 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=0.526253 PRIMER_LEFT_2_PENALTY=0.147295 PRIMER_RIGHT_2_PENALTY=0.378958 PRIMER_INTERNAL_2_PENALTY=1.011075 PRIMER_LEFT_2_SEQUENCE=AAGCAGAACACCGGAAAAGA PRIMER_RIGHT_2_SEQUENCE=AACTCGACAAGAGCCAAAGC PRIMER_INTERNAL_2_SEQUENCE=CCCTCCAAATGGTGTCTCTT PRIMER_LEFT_2=139,20 PRIMER_RIGHT_2=221,20 PRIMER_INTERNAL_2=182,20 PRIMER_LEFT_2_TM=59.853 PRIMER_RIGHT_2_TM=59.621 PRIMER_INTERNAL_2_TM=58.989 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=2.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=0.674472 PRIMER_LEFT_3_PENALTY=0.029253 PRIMER_RIGHT_3_PENALTY=0.645218 PRIMER_INTERNAL_3_PENALTY=0.667795 PRIMER_LEFT_3_SEQUENCE=ACACCCAAGAAAAATGGCAG PRIMER_RIGHT_3_SEQUENCE=CTTTTCCGGTGTTCTGCTTT PRIMER_INTERNAL_3_SEQUENCE=CATCACAGGAGGTCTCAGGG PRIMER_LEFT_3=59,20 PRIMER_RIGHT_3=157,20 PRIMER_INTERNAL_3=93,20 PRIMER_LEFT_3_TM=59.971 PRIMER_RIGHT_3_TM=59.355 PRIMER_INTERNAL_3_TM=60.668 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=0.694913 PRIMER_LEFT_4_PENALTY=0.005232 PRIMER_RIGHT_4_PENALTY=0.689681 PRIMER_INTERNAL_4_PENALTY=0.003322 PRIMER_LEFT_4_SEQUENCE=ACACCGGAAAAGACCTTGTG PRIMER_RIGHT_4_SEQUENCE=CACCTGGTCTTCCAACAAGC PRIMER_INTERNAL_4_SEQUENCE=GGTGTCTCTTGCTTTGGCTC PRIMER_LEFT_4=146,20 PRIMER_RIGHT_4=244,20 PRIMER_INTERNAL_4=192,20 PRIMER_LEFT_4_TM=60.005 PRIMER_RIGHT_4_TM=60.690 PRIMER_INTERNAL_4_TM=59.997 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = SEQUENCE_ID=MH1241 SEQUENCE_TEMPLATE=CTTGCATNCCTCCAGGTCGACTCTAGAGGATCCCCCTGCAGAAAGTGAGAGAAGGAGGGGACAGGACAGGATGTCTGGGCCATCCCGATGGTCCACTTGGTCTGTTCATTTGCTGAGCGGCCTTGTTCCGTGAGTNCCCTGTGGNTNTCCAGGACAACAGATGCACCATCTCTAGTGCCATTTGAAACTAAATGAANGAATGAGGAGGAAAAAAAAAAGGAAACTGGTATCTGCTGCCCAACTGATANNTGCGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGT SEQUENCE_INCLUDED_REGION=35,220 P3_COMMENT=CL is GAAAGTGAGAGAAGGAGGGG P3_COMMENT=CR is ATCAGTTGGGCAGCAGATAC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.578120 PRIMER_LEFT_0_PENALTY=0.129310 PRIMER_RIGHT_0_PENALTY=0.448810 PRIMER_INTERNAL_0_PENALTY=4.255414 PRIMER_LEFT_0_SEQUENCE=TGGTCCACTTGGTCTGTTCA PRIMER_RIGHT_0_SEQUENCE=ATGGTGCATCTGTTGTCCTG PRIMER_INTERNAL_0_SEQUENCE=CGGCCTTGTTCCGTGAGT PRIMER_LEFT_0=88,20 PRIMER_RIGHT_0=168,20 PRIMER_INTERNAL_0=117,18 PRIMER_LEFT_0_TM=60.129 PRIMER_RIGHT_0_TM=59.551 PRIMER_INTERNAL_0_TM=62.255 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=0.590321 PRIMER_LEFT_1_PENALTY=0.448810 PRIMER_RIGHT_1_PENALTY=0.141511 PRIMER_INTERNAL_1_PENALTY=2.756595 PRIMER_LEFT_1_SEQUENCE=CAGGACAACAGATGCACCAT PRIMER_RIGHT_1_SEQUENCE=GTTGGGCAGCAGATACCAGT PRIMER_INTERNAL_1_SEQUENCE=TGAGGAGGAAAAAAAAAAGGAA PRIMER_LEFT_1=149,20 PRIMER_RIGHT_1=241,20 PRIMER_INTERNAL_1=200,22 PRIMER_LEFT_1_TM=59.551 PRIMER_RIGHT_1_TM=60.142 PRIMER_INTERNAL_1_TM=59.243 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=31.818 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.8000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=93 PRIMER_PAIR_2_PENALTY=0.725622 PRIMER_LEFT_2_PENALTY=0.448810 PRIMER_RIGHT_2_PENALTY=0.276812 PRIMER_INTERNAL_2_PENALTY=2.756595 PRIMER_LEFT_2_SEQUENCE=CAGGACAACAGATGCACCAT PRIMER_RIGHT_2_SEQUENCE=AGTTGGGCAGCAGATACCAG PRIMER_INTERNAL_2_SEQUENCE=TGAGGAGGAAAAAAAAAAGGAA PRIMER_LEFT_2=149,20 PRIMER_RIGHT_2=242,20 PRIMER_INTERNAL_2=200,22 PRIMER_LEFT_2_TM=59.551 PRIMER_RIGHT_2_TM=60.277 PRIMER_INTERNAL_2_TM=59.243 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=31.818 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.8000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=94 PRIMER_PAIR_3_PENALTY=0.749053 PRIMER_LEFT_3_PENALTY=0.448810 PRIMER_RIGHT_3_PENALTY=0.300243 PRIMER_INTERNAL_3_PENALTY=5.113576 PRIMER_LEFT_3_SEQUENCE=CAGGACAACAGATGCACCAT PRIMER_RIGHT_3_SEQUENCE=GGCAGCAGATACCAGTTTCC PRIMER_INTERNAL_3_SEQUENCE=AGTGCCATTTGAAACTAAATGAA PRIMER_LEFT_3=149,20 PRIMER_RIGHT_3=237,20 PRIMER_INTERNAL_3=173,23 PRIMER_LEFT_3_TM=59.551 PRIMER_RIGHT_3_TM=59.700 PRIMER_INTERNAL_3_TM=57.886 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=30.435 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.8000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=89 PRIMER_PAIR_4_PENALTY=0.749053 PRIMER_LEFT_4_PENALTY=0.448810 PRIMER_RIGHT_4_PENALTY=0.300243 PRIMER_INTERNAL_4_PENALTY=5.113576 PRIMER_LEFT_4_SEQUENCE=CAGGACAACAGATGCACCAT PRIMER_RIGHT_4_SEQUENCE=GGGCAGCAGATACCAGTTTC PRIMER_INTERNAL_4_SEQUENCE=AGTGCCATTTGAAACTAAATGAA PRIMER_LEFT_4=149,20 PRIMER_RIGHT_4=238,20 PRIMER_INTERNAL_4=173,23 PRIMER_LEFT_4_TM=59.551 PRIMER_RIGHT_4_TM=59.700 PRIMER_INTERNAL_4_TM=57.886 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=30.435 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=7.8000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=90 = SEQUENCE_ID=MH1242 SEQUENCE_TEMPLATE=TCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTTTACTAGCCTGAGTNTTTTGTATATGAAATGAGAACAATGACCAATAGTGAGAAGACTTTTTTGTAGGACTGAGTNNAATAATACATGCAAAGACCCCAGCCCCTCAGTGCTCTCAGGTCAACACATATTAATTTCCTTCGTCTCTCTTTGAAGGTCAGAAAATTTGGACAGTGGCGTAGAATTACAGTGCTGTGGGCCAGTGTGCCATNTGATGGCAGAAAGCACTNNTTGGATCTCTCTGCATGT SEQUENCE_INCLUDED_REGION=36,250 P3_COMMENT=CL is TGTATATGAAATGAGAACAATGACC P3_COMMENT=CR is TACGCCACTGTCCAAATTTT P3_COMMENT=CM is GTGAGAAGACTTTTTTGTAGGACTG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.060277 PRIMER_LEFT_0_PENALTY=0.037936 PRIMER_RIGHT_0_PENALTY=0.022341 PRIMER_INTERNAL_0_PENALTY=0.875462 PRIMER_LEFT_0_SEQUENCE=ATACATGCAAAGACCCCAGC PRIMER_RIGHT_0_SEQUENCE=ACGCCACTGTCCAAATTTTC PRIMER_INTERNAL_0_SEQUENCE=CCTCAGTGCTCTCAGGTCAA PRIMER_LEFT_0=120,20 PRIMER_RIGHT_0=216,20 PRIMER_INTERNAL_0=141,20 PRIMER_LEFT_0_TM=59.962 PRIMER_RIGHT_0_TM=59.978 PRIMER_INTERNAL_0_TM=59.125 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=97 PRIMER_PAIR_1_PENALTY=0.148328 PRIMER_LEFT_1_PENALTY=0.037936 PRIMER_RIGHT_1_PENALTY=0.110392 PRIMER_INTERNAL_1_PENALTY=0.875462 PRIMER_LEFT_1_SEQUENCE=ATACATGCAAAGACCCCAGC PRIMER_RIGHT_1_SEQUENCE=CGCCACTGTCCAAATTTTCT PRIMER_INTERNAL_1_SEQUENCE=CCTCAGTGCTCTCAGGTCAA PRIMER_LEFT_1=120,20 PRIMER_RIGHT_1=215,20 PRIMER_INTERNAL_1=141,20 PRIMER_LEFT_1_TM=59.962 PRIMER_RIGHT_1_TM=60.110 PRIMER_INTERNAL_1_TM=59.125 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=0.897803 PRIMER_LEFT_2_PENALTY=0.875462 PRIMER_RIGHT_2_PENALTY=0.022341 PRIMER_INTERNAL_2_PENALTY=2.008930 PRIMER_LEFT_2_SEQUENCE=CCTCAGTGCTCTCAGGTCAA PRIMER_RIGHT_2_SEQUENCE=ACGCCACTGTCCAAATTTTC PRIMER_INTERNAL_2_SEQUENCE=TTCCTTCGTCTCTCTTTGAAGG PRIMER_LEFT_2=141,20 PRIMER_RIGHT_2=216,20 PRIMER_INTERNAL_2=172,22 PRIMER_LEFT_2_TM=59.125 PRIMER_RIGHT_2_TM=59.978 PRIMER_INTERNAL_2_TM=59.991 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.3000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=76 PRIMER_PAIR_3_PENALTY=0.985854 PRIMER_LEFT_3_PENALTY=0.875462 PRIMER_RIGHT_3_PENALTY=0.110392 PRIMER_INTERNAL_3_PENALTY=2.008930 PRIMER_LEFT_3_SEQUENCE=CCTCAGTGCTCTCAGGTCAA PRIMER_RIGHT_3_SEQUENCE=CGCCACTGTCCAAATTTTCT PRIMER_INTERNAL_3_SEQUENCE=TTCCTTCGTCTCTCTTTGAAGG PRIMER_LEFT_3=141,20 PRIMER_RIGHT_3=215,20 PRIMER_INTERNAL_3=172,22 PRIMER_LEFT_3_TM=59.125 PRIMER_RIGHT_3_TM=60.110 PRIMER_INTERNAL_3_TM=59.991 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.455 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=1.048926 PRIMER_LEFT_4_PENALTY=0.037936 PRIMER_RIGHT_4_PENALTY=1.010990 PRIMER_INTERNAL_4_PENALTY=0.875462 PRIMER_LEFT_4_SEQUENCE=ATACATGCAAAGACCCCAGC PRIMER_RIGHT_4_SEQUENCE=TACGCCACTGTCCAAATTTTC PRIMER_INTERNAL_4_SEQUENCE=CCTCAGTGCTCTCAGGTCAA PRIMER_LEFT_4=120,20 PRIMER_RIGHT_4=217,21 PRIMER_INTERNAL_4=141,20 PRIMER_LEFT_4_TM=59.962 PRIMER_RIGHT_4_TM=59.989 PRIMER_INTERNAL_4_TM=59.125 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=7.3000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH1244 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGGCATTTGGCTGCTCTCCAGTCATTTAATCTGGNATTTAATNACAAATAATAGTTNTATTGAGTNNCACAAAGTCAATNCTTCTTACACTCTTTTATTCAGGCAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC SEQUENCE_INCLUDED_REGION=36,109 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH1245 SEQUENCE_TEMPLATE=TGCNTGCCTCCAGGTCGACTCTAGAGGATCCCCTGGCATAATACATAAGATTTAGCACTTTGAATATAATGTTTTTTCATTGAGAACATTATTTTTCTTANNAANACAACCAATATTTTTGTAATTTATAATTAATATATGACAATCCCTGTAATTCTGGTATCAGAAGATCTGGTAAAATCTAATATGTTCCGTGTGTTGTAAGATTTTNNCTTTCTGAATATAAATAANGTCATAGTATCATTGAATGCTTTTATTCATTCAAAATCAGTTATTGAAGCAATCTCTGGGTAGCTATCCAAATATCCTCTTGCAG SEQUENCE_INCLUDED_REGION=33,283 P3_COMMENT=CL is TGGCATAATACATAAGATTTAGCA P3_COMMENT=CR is GCTACCCAGAGATTGCTTCA P3_COMMENT=CM is TATATGACAATCCCTGTAATTCTGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=8.961298 PRIMER_LEFT_0_PENALTY=5.323258 PRIMER_RIGHT_0_PENALTY=3.638040 PRIMER_INTERNAL_0_PENALTY=3.135712 PRIMER_LEFT_0_SEQUENCE=TCATTGAATGCTTTTATTCATTCA PRIMER_RIGHT_0_SEQUENCE=TGCAAGAGGATATTTGGATAGC PRIMER_INTERNAL_0_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG PRIMER_LEFT_0=240,24 PRIMER_RIGHT_0=314,22 PRIMER_INTERNAL_0=268,23 PRIMER_LEFT_0_TM=58.677 PRIMER_RIGHT_0_TM=58.362 PRIMER_INTERNAL_0_TM=60.136 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=40.909 PRIMER_INTERNAL_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=8.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=7.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=9.038563 PRIMER_LEFT_1_PENALTY=5.323258 PRIMER_RIGHT_1_PENALTY=3.715305 PRIMER_INTERNAL_1_PENALTY=3.135712 PRIMER_LEFT_1_SEQUENCE=TCATTGAATGCTTTTATTCATTCA PRIMER_RIGHT_1_SEQUENCE=TGCAAGAGGATATTTGGATAGCT PRIMER_INTERNAL_1_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG PRIMER_LEFT_1=240,24 PRIMER_RIGHT_1=314,23 PRIMER_INTERNAL_1=268,23 PRIMER_LEFT_1_TM=58.677 PRIMER_RIGHT_1_TM=59.285 PRIMER_INTERNAL_1_TM=60.136 PRIMER_LEFT_1_GC_PERCENT=25.000 PRIMER_RIGHT_1_GC_PERCENT=39.130 PRIMER_INTERNAL_1_GC_PERCENT=43.478 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=8.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=7.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=9.038563 PRIMER_LEFT_2_PENALTY=5.323258 PRIMER_RIGHT_2_PENALTY=3.715305 PRIMER_INTERNAL_2_PENALTY=3.135712 PRIMER_LEFT_2_SEQUENCE=TCATTGAATGCTTTTATTCATTCA PRIMER_RIGHT_2_SEQUENCE=CTGCAAGAGGATATTTGGATAGC PRIMER_INTERNAL_2_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG PRIMER_LEFT_2=240,24 PRIMER_RIGHT_2=315,23 PRIMER_INTERNAL_2=268,23 PRIMER_LEFT_2_TM=58.677 PRIMER_RIGHT_2_TM=59.285 PRIMER_INTERNAL_2_TM=60.136 PRIMER_LEFT_2_GC_PERCENT=25.000 PRIMER_RIGHT_2_GC_PERCENT=43.478 PRIMER_INTERNAL_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=8.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=7.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=76 PRIMER_PAIR_3_PENALTY=9.267901 PRIMER_LEFT_3_PENALTY=5.552596 PRIMER_RIGHT_3_PENALTY=3.715305 PRIMER_INTERNAL_3_PENALTY=3.135712 PRIMER_LEFT_3_SEQUENCE=CATTGAATGCTTTTATTCATTCAAA PRIMER_RIGHT_3_SEQUENCE=CTGCAAGAGGATATTTGGATAGC PRIMER_INTERNAL_3_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG PRIMER_LEFT_3=241,25 PRIMER_RIGHT_3=315,23 PRIMER_INTERNAL_3=268,23 PRIMER_LEFT_3_TM=59.447 PRIMER_RIGHT_3_TM=59.285 PRIMER_INTERNAL_3_TM=60.136 PRIMER_LEFT_3_GC_PERCENT=24.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_INTERNAL_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=10.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=8.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=7.1000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=9.456046 PRIMER_LEFT_4_PENALTY=5.323258 PRIMER_RIGHT_4_PENALTY=4.132788 PRIMER_INTERNAL_4_PENALTY=3.135712 PRIMER_LEFT_4_SEQUENCE=TCATTGAATGCTTTTATTCATTCA PRIMER_RIGHT_4_SEQUENCE=CTGCAAGAGGATATTTGGATAGCT PRIMER_INTERNAL_4_SEQUENCE=CAGTTATTGAAGCAATCTCTGGG PRIMER_LEFT_4=240,24 PRIMER_RIGHT_4=315,24 PRIMER_INTERNAL_4=268,23 PRIMER_LEFT_4_TM=58.677 PRIMER_RIGHT_4_TM=60.133 PRIMER_INTERNAL_4_TM=60.136 PRIMER_LEFT_4_GC_PERCENT=25.000 PRIMER_RIGHT_4_GC_PERCENT=41.667 PRIMER_INTERNAL_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=8.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=7.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH1246 SEQUENCE_TEMPLATE=NNNTGAGNTCGCNTGCCTNCAGGTCGACTCTAGAGGATCCCTCCACTGCTGTNNTTCACACAGTNGGGNATGCTGCAACCTTAGGGCCTTTTCACTCTTCTCTCTTCATAGAACACTCCTCCCTGAGACAGCCATGTAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCG SEQUENCE_INCLUDED_REGION=41,97 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH1247 SEQUENCE_TEMPLATE=NNTCCCTCCAGGTCGACTCTAGAGGNNCCCCTTACATCTCCATAAGCAGCATGTGTNTTAATTGCTGAGCCCCAGTNACCACCAGACAATCTAATCAATGTCTCCATAATTAACCTCACCAAATGGAAAGTCATAAATTTAGAACTAAAATGAAATTCTGCTCAGTCTGCATATAGAAACTGACATAGCCCAAAAGGAGACAACAGGGTNNTTACCAGAATAATTTAAGAAAACCTTGTTTGGAAGGGAAGGAAAACAAAATAATGTGATACCAAAATGACCAGATGGATCATATACATTAATCGTAGTTGCTGGGTGNCTGCTGGAAAAATATGTCTCTCACA SEQUENCE_INCLUDED_REGION=31,313 P3_COMMENT=CL is TCTCCATAAGCAGCATGTGT P3_COMMENT=CR is GTGAGAGACATATTTTTCCAGC P3_COMMENT=CM is ACCACCAGACAATCTAATCAATG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.117963 PRIMER_LEFT_0_PENALTY=1.635755 PRIMER_RIGHT_0_PENALTY=0.482208 PRIMER_INTERNAL_0_PENALTY=8.377222 PRIMER_LEFT_0_SEQUENCE=TAGCCCAAAAGGAGACAACAG PRIMER_RIGHT_0_SEQUENCE=TTTGTTTTCCTTCCCTTCCA PRIMER_INTERNAL_0_SEQUENCE=CCAGAATAATTTAAGAAAACCTTGTT PRIMER_LEFT_0=185,21 PRIMER_RIGHT_0=259,20 PRIMER_INTERNAL_0=214,26 PRIMER_LEFT_0_TM=59.364 PRIMER_RIGHT_0_TM=59.518 PRIMER_INTERNAL_0_TM=57.623 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=26.923 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=2.130360 PRIMER_LEFT_1_PENALTY=1.458762 PRIMER_RIGHT_1_PENALTY=0.671597 PRIMER_INTERNAL_1_PENALTY=1.269110 PRIMER_LEFT_1_SEQUENCE=CCTCACCAAATGGAAAGTCA PRIMER_RIGHT_1_SEQUENCE=CTGTTGTCTCCTTTTGGGCT PRIMER_INTERNAL_1_SEQUENCE=TGAAATTCTGCTCAGTCTGCA PRIMER_LEFT_1=113,20 PRIMER_RIGHT_1=205,20 PRIMER_INTERNAL_1=150,21 PRIMER_LEFT_1_TM=58.541 PRIMER_RIGHT_1_TM=59.328 PRIMER_INTERNAL_1_TM=59.731 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=10.9000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=93 PRIMER_PAIR_2_PENALTY=2.130608 PRIMER_LEFT_2_PENALTY=1.648400 PRIMER_RIGHT_2_PENALTY=0.482208 PRIMER_INTERNAL_2_PENALTY=8.377222 PRIMER_LEFT_2_SEQUENCE=TAGCCCAAAAGGAGACAACA PRIMER_RIGHT_2_SEQUENCE=TTTGTTTTCCTTCCCTTCCA PRIMER_INTERNAL_2_SEQUENCE=CCAGAATAATTTAAGAAAACCTTGTT PRIMER_LEFT_2=185,20 PRIMER_RIGHT_2=259,20 PRIMER_INTERNAL_2=214,26 PRIMER_LEFT_2_TM=58.352 PRIMER_RIGHT_2_TM=59.518 PRIMER_INTERNAL_2_TM=57.623 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=26.923 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=2.160491 PRIMER_LEFT_3_PENALTY=1.678283 PRIMER_RIGHT_3_PENALTY=0.482208 PRIMER_INTERNAL_3_PENALTY=9.300837 PRIMER_LEFT_3_SEQUENCE=CTGACATAGCCCAAAAGGAGA PRIMER_RIGHT_3_SEQUENCE=TGTTTTCCTTCCCTTCCAAA PRIMER_INTERNAL_3_SEQUENCE=TTACCAGAATAATTTAAGAAAACCTTG PRIMER_LEFT_3=179,21 PRIMER_RIGHT_3=257,20 PRIMER_INTERNAL_3=211,27 PRIMER_LEFT_3_TM=59.322 PRIMER_RIGHT_3_TM=59.518 PRIMER_INTERNAL_3_TM=57.699 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=25.926 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.8000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=2.160491 PRIMER_LEFT_4_PENALTY=1.678283 PRIMER_RIGHT_4_PENALTY=0.482208 PRIMER_INTERNAL_4_PENALTY=8.800552 PRIMER_LEFT_4_SEQUENCE=CTGACATAGCCCAAAAGGAGA PRIMER_RIGHT_4_SEQUENCE=TTGTTTTCCTTCCCTTCCAA PRIMER_INTERNAL_4_SEQUENCE=ACCAGAATAATTTAAGAAAACCTTGT PRIMER_LEFT_4=179,21 PRIMER_RIGHT_4=258,20 PRIMER_INTERNAL_4=213,26 PRIMER_LEFT_4_TM=59.322 PRIMER_RIGHT_4_TM=59.518 PRIMER_INTERNAL_4_TM=57.199 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=26.923 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH1248 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCTTCTCTTTCTCAGCCTGTTTTCCTCATCTGTAAAATAAAAGGGTTGTATCAGACAATTTCTATTATCTTTCTCTAACTCATTTTGTCTTATATAGGTATAAATGAAAATACAAGAAATTGTCTTTAATCTGGACAAGTTCTATAGGACACACCGTGCTGAATCTTACAGAGAAGNACTTGGGTTAGAAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGA SEQUENCE_INCLUDED_REGION=36,191 P3_COMMENT=CL is CAGCCTGTTTTCCTCATCTG P3_COMMENT=CR is TCAGCACGGTGTGTCCTATA P3_COMMENT=CM is AAAATAAAAGGGTTGTATCAGACAA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=7.100570 PRIMER_LEFT_0_PENALTY=6.265639 PRIMER_RIGHT_0_PENALTY=0.834931 PRIMER_INTERNAL_0_PENALTY=6.056317 PRIMER_LEFT_0_SEQUENCE=AAAAGGGTTGTATCAGACAATTTC PRIMER_RIGHT_0_SEQUENCE=TTCAGCACGGTGTGTCCTAT PRIMER_INTERNAL_0_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA PRIMER_LEFT_0=75,24 PRIMER_RIGHT_0=199,20 PRIMER_INTERNAL_0=149,26 PRIMER_LEFT_0_TM=57.734 PRIMER_RIGHT_0_TM=59.165 PRIMER_INTERNAL_0_TM=59.944 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=30.769 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=7.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=125 PRIMER_PAIR_1_PENALTY=7.210709 PRIMER_LEFT_1_PENALTY=6.375778 PRIMER_RIGHT_1_PENALTY=0.834931 PRIMER_INTERNAL_1_PENALTY=6.056317 PRIMER_LEFT_1_SEQUENCE=AAAAGGGTTGTATCAGACAATTTCT PRIMER_RIGHT_1_SEQUENCE=TTCAGCACGGTGTGTCCTAT PRIMER_INTERNAL_1_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA PRIMER_LEFT_1=75,25 PRIMER_RIGHT_1=199,20 PRIMER_INTERNAL_1=149,26 PRIMER_LEFT_1_TM=58.624 PRIMER_RIGHT_1_TM=59.165 PRIMER_INTERNAL_1_TM=59.944 PRIMER_LEFT_1_GC_PERCENT=32.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=30.769 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=7.1000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=125 PRIMER_PAIR_2_PENALTY=7.443073 PRIMER_LEFT_2_PENALTY=6.608142 PRIMER_RIGHT_2_PENALTY=0.834931 PRIMER_INTERNAL_2_PENALTY=6.056317 PRIMER_LEFT_2_SEQUENCE=AAAGGGTTGTATCAGACAATTTCT PRIMER_RIGHT_2_SEQUENCE=TTCAGCACGGTGTGTCCTAT PRIMER_INTERNAL_2_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA PRIMER_LEFT_2=76,24 PRIMER_RIGHT_2=199,20 PRIMER_INTERNAL_2=149,26 PRIMER_LEFT_2_TM=57.392 PRIMER_RIGHT_2_TM=59.165 PRIMER_INTERNAL_2_TM=59.944 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=30.769 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=7.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=124 PRIMER_PAIR_3_PENALTY=7.443073 PRIMER_LEFT_3_PENALTY=6.608142 PRIMER_RIGHT_3_PENALTY=0.834931 PRIMER_INTERNAL_3_PENALTY=6.056317 PRIMER_LEFT_3_SEQUENCE=AAAGGGTTGTATCAGACAATTTCT PRIMER_RIGHT_3_SEQUENCE=ATTCAGCACGGTGTGTCCTA PRIMER_INTERNAL_3_SEQUENCE=CAAGAAATTGTCTTTAATCTGGACAA PRIMER_LEFT_3=76,24 PRIMER_RIGHT_3=200,20 PRIMER_INTERNAL_3=149,26 PRIMER_LEFT_3_TM=57.392 PRIMER_RIGHT_3_TM=59.165 PRIMER_INTERNAL_3_TM=59.944 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=30.769 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=125 PRIMER_PAIR_4_PENALTY=7.768938 PRIMER_LEFT_4_PENALTY=1.558232 PRIMER_RIGHT_4_PENALTY=6.210707 PRIMER_INTERNAL_4_PENALTY=6.265639 PRIMER_LEFT_4_SEQUENCE=CAGCCTGTTTTCCTCATCTG PRIMER_RIGHT_4_SEQUENCE=TGTCCAGATTAAAGACAATTTCTTG PRIMER_INTERNAL_4_SEQUENCE=AAAAGGGTTGTATCAGACAATTTC PRIMER_LEFT_4=49,20 PRIMER_RIGHT_4=173,25 PRIMER_INTERNAL_4=75,24 PRIMER_LEFT_4_TM=58.442 PRIMER_RIGHT_4_TM=58.789 PRIMER_INTERNAL_4_TM=57.734 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=32.000 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=125 = SEQUENCE_ID=MH1250 SEQUENCE_TEMPLATE=CTCTTCACTCCAGGTCGACTCTAGAGGATCCCTCTGTTATNTATAATTTNATTTGGAAAACTTAAACCTAGACAGGGATCATAACTTGGAAATCATCAAGTTGAAAAAAGAATCATAGATGGCCTCTGGAATTTNGTTCAAAAGAANACTGGAAACTGAAAATTNNGGGACTCATTTAAGTCATGTATTCAGTTTTTTAAAATATCTTTAAGANCNNTANNNNNNCCAGAGAAAAGTTGTCTATCTGTATGTGTGTGNGNGTGTGTGTTTGTGTGTGCACAC SEQUENCE_INCLUDED_REGION=32,250 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.972563 PRIMER_LEFT_0_PENALTY=4.066464 PRIMER_RIGHT_0_PENALTY=1.906099 PRIMER_INTERNAL_0_PENALTY=4.134716 PRIMER_LEFT_0_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG PRIMER_RIGHT_0_SEQUENCE=TCCAGAGGCCATCTATGATTC PRIMER_INTERNAL_0_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA PRIMER_LEFT_0=53,24 PRIMER_RIGHT_0=129,21 PRIMER_INTERNAL_0=85,24 PRIMER_LEFT_0_TM=59.934 PRIMER_RIGHT_0_TM=59.094 PRIMER_INTERNAL_0_TM=59.865 PRIMER_LEFT_0_GC_PERCENT=41.667 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_INTERNAL_0_GC_PERCENT=25.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=6.126660 PRIMER_LEFT_1_PENALTY=4.066464 PRIMER_RIGHT_1_PENALTY=2.060196 PRIMER_INTERNAL_1_PENALTY=4.359729 PRIMER_LEFT_1_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG PRIMER_RIGHT_1_SEQUENCE=TCCAGAGGCCATCTATGATTCT PRIMER_INTERNAL_1_SEQUENCE=TTGGAAATCATCAAGTTGAAAAA PRIMER_LEFT_1=53,24 PRIMER_RIGHT_1=129,22 PRIMER_INTERNAL_1=85,23 PRIMER_LEFT_1_TM=59.934 PRIMER_RIGHT_1_TM=60.060 PRIMER_INTERNAL_1_TM=58.640 PRIMER_LEFT_1_GC_PERCENT=41.667 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_INTERNAL_1_GC_PERCENT=26.087 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=6.135990 PRIMER_LEFT_2_PENALTY=4.066464 PRIMER_RIGHT_2_PENALTY=2.069526 PRIMER_INTERNAL_2_PENALTY=4.134716 PRIMER_LEFT_2_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG PRIMER_RIGHT_2_SEQUENCE=AAATTCCAGAGGCCATCTATGA PRIMER_INTERNAL_2_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA PRIMER_LEFT_2=53,24 PRIMER_RIGHT_2=133,22 PRIMER_INTERNAL_2=85,24 PRIMER_LEFT_2_TM=59.934 PRIMER_RIGHT_2_TM=59.930 PRIMER_INTERNAL_2_TM=59.865 PRIMER_LEFT_2_GC_PERCENT=41.667 PRIMER_RIGHT_2_GC_PERCENT=40.909 PRIMER_INTERNAL_2_GC_PERCENT=25.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=5.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=6.456280 PRIMER_LEFT_3_PENALTY=4.066464 PRIMER_RIGHT_3_PENALTY=2.389816 PRIMER_INTERNAL_3_PENALTY=4.134716 PRIMER_LEFT_3_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG PRIMER_RIGHT_3_SEQUENCE=TTCCAGAGGCCATCTATGATT PRIMER_INTERNAL_3_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA PRIMER_LEFT_3=53,24 PRIMER_RIGHT_3=130,21 PRIMER_INTERNAL_3=85,24 PRIMER_LEFT_3_TM=59.934 PRIMER_RIGHT_3_TM=58.610 PRIMER_INTERNAL_3_TM=59.865 PRIMER_LEFT_3_GC_PERCENT=41.667 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_INTERNAL_3_GC_PERCENT=25.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=78 PRIMER_PAIR_4_PENALTY=6.456280 PRIMER_LEFT_4_PENALTY=4.066464 PRIMER_RIGHT_4_PENALTY=2.389816 PRIMER_INTERNAL_4_PENALTY=4.134716 PRIMER_LEFT_4_SEQUENCE=TGGAAAACTTAAACCTAGACAGGG PRIMER_RIGHT_4_SEQUENCE=AATTCCAGAGGCCATCTATGA PRIMER_INTERNAL_4_SEQUENCE=TTGGAAATCATCAAGTTGAAAAAA PRIMER_LEFT_4=53,24 PRIMER_RIGHT_4=132,21 PRIMER_INTERNAL_4=85,24 PRIMER_LEFT_4_TM=59.934 PRIMER_RIGHT_4_TM=58.610 PRIMER_INTERNAL_4_TM=59.865 PRIMER_LEFT_4_GC_PERCENT=41.667 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=25.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=5.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH1251 SEQUENCE_TEMPLATE=GCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTAAAGGAGTNNGCCACATGGATGATTGGAGAAAGTGCATCCTAGGCAGAGGANACAGCAAGTGCAAATGCCTTAAGATAGCGTAAGTCTAGGGAGTGTGAGATTTGCAAGGATGCAGGGTAATTGAAGAAAAGTGAGTAAAGGAGCCAGAAAGAGATGGATGTAGAGAGGTATCCAGTGGCCAGACCATACATAGCCCTGTGAGCCTTTTTAAGGAGGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACA SEQUENCE_INCLUDED_REGION=36,218 P3_COMMENT=CL is CCACATGGATGATTGGAGAA P3_COMMENT=CR is TAAAAAGGCTCACAGGGCTA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.078512 PRIMER_LEFT_0_PENALTY=0.026532 PRIMER_RIGHT_0_PENALTY=0.051981 PRIMER_INTERNAL_0_PENALTY=0.014163 PRIMER_LEFT_0_SEQUENCE=AGTGCATCCTAGGCAGAGGA PRIMER_RIGHT_0_SEQUENCE=CTGCATCCTTGCAAATCTCA PRIMER_INTERNAL_0_SEQUENCE=CAGCAAGTGCAAATGCCTTA PRIMER_LEFT_0=69,20 PRIMER_RIGHT_0=153,20 PRIMER_INTERNAL_0=91,20 PRIMER_LEFT_0_TM=59.973 PRIMER_RIGHT_0_TM=59.948 PRIMER_INTERNAL_0_TM=60.014 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=85 PRIMER_PAIR_1_PENALTY=0.221130 PRIMER_LEFT_1_PENALTY=0.026532 PRIMER_RIGHT_1_PENALTY=0.194598 PRIMER_INTERNAL_1_PENALTY=0.014163 PRIMER_LEFT_1_SEQUENCE=AGTGCATCCTAGGCAGAGGA PRIMER_RIGHT_1_SEQUENCE=GCATCCTTGCAAATCTCACA PRIMER_INTERNAL_1_SEQUENCE=CAGCAAGTGCAAATGCCTTA PRIMER_LEFT_1=69,20 PRIMER_RIGHT_1=151,20 PRIMER_INTERNAL_1=91,20 PRIMER_LEFT_1_TM=59.973 PRIMER_RIGHT_1_TM=59.805 PRIMER_INTERNAL_1_TM=60.014 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=0.221130 PRIMER_LEFT_2_PENALTY=0.026532 PRIMER_RIGHT_2_PENALTY=0.194598 PRIMER_INTERNAL_2_PENALTY=0.014163 PRIMER_LEFT_2_SEQUENCE=AGTGCATCCTAGGCAGAGGA PRIMER_RIGHT_2_SEQUENCE=TGCATCCTTGCAAATCTCAC PRIMER_INTERNAL_2_SEQUENCE=CAGCAAGTGCAAATGCCTTA PRIMER_LEFT_2=69,20 PRIMER_RIGHT_2=152,20 PRIMER_INTERNAL_2=91,20 PRIMER_LEFT_2_TM=59.973 PRIMER_RIGHT_2_TM=59.805 PRIMER_INTERNAL_2_TM=60.014 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=84 PRIMER_PAIR_3_PENALTY=0.242322 PRIMER_LEFT_3_PENALTY=0.051981 PRIMER_RIGHT_3_PENALTY=0.190341 PRIMER_INTERNAL_3_PENALTY=1.033644 PRIMER_LEFT_3_SEQUENCE=TGAGATTTGCAAGGATGCAG PRIMER_RIGHT_3_SEQUENCE=ATGGTCTGGCCACTGGATAC PRIMER_INTERNAL_3_SEQUENCE=AGGAGCCAGAAAGAGATGGA PRIMER_LEFT_3=134,20 PRIMER_RIGHT_3=225,20 PRIMER_INTERNAL_3=177,20 PRIMER_LEFT_3_TM=59.948 PRIMER_RIGHT_3_TM=59.810 PRIMER_INTERNAL_3_TM=58.966 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=5.3000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=92 PRIMER_PAIR_4_PENALTY=0.242322 PRIMER_LEFT_4_PENALTY=0.051981 PRIMER_RIGHT_4_PENALTY=0.190341 PRIMER_INTERNAL_4_PENALTY=1.033644 PRIMER_LEFT_4_SEQUENCE=TGAGATTTGCAAGGATGCAG PRIMER_RIGHT_4_SEQUENCE=GTATGGTCTGGCCACTGGAT PRIMER_INTERNAL_4_SEQUENCE=AGGAGCCAGAAAGAGATGGA PRIMER_LEFT_4=134,20 PRIMER_RIGHT_4=227,20 PRIMER_INTERNAL_4=177,20 PRIMER_LEFT_4_TM=59.948 PRIMER_RIGHT_4_TM=59.810 PRIMER_INTERNAL_4_TM=58.966 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=94 = SEQUENCE_ID=MH1254 SEQUENCE_TEMPLATE=CTTTCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTGGCCTACCTCTCACTCCCTCTAGCCAGCNTACCTCTGGCCAGTNTCCTGGCAGGCCATGCATTTCTAGCCCTCTCCACCTGTGCATGTCCTGTCTCCTCCCCGTTCTTCTCTGCCAAACTAATTTCTGTCCTCCTCATGTGGAGCCTGGACCCCCACCTTGAGGCCAGTGCCCATTTTCTGTGGCCCCATTGCACCTTCTAGCAGAACCCTTAAACTGCACACAGCAAGGGGTACNGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTC SEQUENCE_INCLUDED_REGION=35,231 P3_COMMENT=CL is TACCTCTCACTCCCTCTAGCC P3_COMMENT=CR is GGTTCTGCTAGAAGGTGCAA P3_COMMENT=CM is GGCAGGCCATGCATTTCTAG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.388917 PRIMER_LEFT_0_PENALTY=0.216148 PRIMER_RIGHT_0_PENALTY=0.172769 PRIMER_INTERNAL_0_PENALTY=0.707509 PRIMER_LEFT_0_SEQUENCE=GGCCTACCTCTCACTCCCTC PRIMER_RIGHT_0_SEQUENCE=GAGGAGACAGGACATGCACA PRIMER_INTERNAL_0_SEQUENCE=CCATGCATTTCTAGCCCTCT PRIMER_LEFT_0=37,20 PRIMER_RIGHT_0=135,20 PRIMER_INTERNAL_0=91,20 PRIMER_LEFT_0_TM=60.216 PRIMER_RIGHT_0_TM=59.827 PRIMER_INTERNAL_0_TM=59.292 PRIMER_LEFT_0_GC_PERCENT=65.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=99 PRIMER_PAIR_1_PENALTY=0.469491 PRIMER_LEFT_1_PENALTY=0.296722 PRIMER_RIGHT_1_PENALTY=0.172769 PRIMER_INTERNAL_1_PENALTY=0.707509 PRIMER_LEFT_1_SEQUENCE=TCTCACTCCCTCTAGCCAGC PRIMER_RIGHT_1_SEQUENCE=GAGGAGACAGGACATGCACA PRIMER_INTERNAL_1_SEQUENCE=CCATGCATTTCTAGCCCTCT PRIMER_LEFT_1=45,20 PRIMER_RIGHT_1=135,20 PRIMER_INTERNAL_1=91,20 PRIMER_LEFT_1_TM=59.703 PRIMER_RIGHT_1_TM=59.827 PRIMER_INTERNAL_1_TM=59.292 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=0.478476 PRIMER_LEFT_2_PENALTY=0.216148 PRIMER_RIGHT_2_PENALTY=0.262328 PRIMER_INTERNAL_2_PENALTY=0.707509 PRIMER_LEFT_2_SEQUENCE=GGCCTACCTCTCACTCCCTC PRIMER_RIGHT_2_SEQUENCE=AGACAGGACATGCACAGGTG PRIMER_INTERNAL_2_SEQUENCE=CCATGCATTTCTAGCCCTCT PRIMER_LEFT_2=37,20 PRIMER_RIGHT_2=131,20 PRIMER_INTERNAL_2=91,20 PRIMER_LEFT_2_TM=60.216 PRIMER_RIGHT_2_TM=59.738 PRIMER_INTERNAL_2_TM=59.292 PRIMER_LEFT_2_GC_PERCENT=65.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=95 PRIMER_PAIR_3_PENALTY=0.559051 PRIMER_LEFT_3_PENALTY=0.296722 PRIMER_RIGHT_3_PENALTY=0.262328 PRIMER_INTERNAL_3_PENALTY=0.707509 PRIMER_LEFT_3_SEQUENCE=TCTCACTCCCTCTAGCCAGC PRIMER_RIGHT_3_SEQUENCE=AGACAGGACATGCACAGGTG PRIMER_INTERNAL_3_SEQUENCE=CCATGCATTTCTAGCCCTCT PRIMER_LEFT_3=45,20 PRIMER_RIGHT_3=131,20 PRIMER_INTERNAL_3=91,20 PRIMER_LEFT_3_TM=59.703 PRIMER_RIGHT_3_TM=59.738 PRIMER_INTERNAL_3_TM=59.292 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=87 PRIMER_PAIR_4_PENALTY=0.623612 PRIMER_LEFT_4_PENALTY=0.450843 PRIMER_RIGHT_4_PENALTY=0.172769 PRIMER_INTERNAL_4_PENALTY=0.707509 PRIMER_LEFT_4_SEQUENCE=CCTCTCACTCCCTCTAGCCA PRIMER_RIGHT_4_SEQUENCE=GAGGAGACAGGACATGCACA PRIMER_INTERNAL_4_SEQUENCE=CCATGCATTTCTAGCCCTCT PRIMER_LEFT_4=43,20 PRIMER_RIGHT_4=135,20 PRIMER_INTERNAL_4=91,20 PRIMER_LEFT_4_TM=59.549 PRIMER_RIGHT_4_TM=59.827 PRIMER_INTERNAL_4_TM=59.292 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=93 = SEQUENCE_ID=MH1258 SEQUENCE_TEMPLATE=CTTGNATGCCTCCAGGTCGACTCTAGAGGATCCCCTGTAATATTGGTAGGAACAAATTCTTGTGCTAGATCCTATGTNGAGTNTAGACNGGCACTCATGGAAAAAGCCTGTGAGTTCCTCCCAGCAATGACTACTGGGACTTTGTACTAAACCATATCCTTTCAAGGGGCAATTATCAGCTTTCTATTGGNTATTAATTGAAACTACTGCAAAGACTAAAGGACATCAAATAATCTTGAAACTTGAAATACCCATGTTGTCATGGTGATGTAAGAGAAACATTTTAATGGGGATAGCAGTGCCCAGAAAAGTTTCATAATAAAAATGGGAAGTGGTTTATAGGGAATCATGCTGTGTGGG SEQUENCE_INCLUDED_REGION=35,325 P3_COMMENT=CL is GAACAAATTCTTGTGCTAGATCC P3_COMMENT=CR is ACAGCATGATTCCCTATAAACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.555836 PRIMER_LEFT_0_PENALTY=0.077340 PRIMER_RIGHT_0_PENALTY=0.478496 PRIMER_INTERNAL_0_PENALTY=5.566536 PRIMER_LEFT_0_SEQUENCE=TTAATGGGGATAGCAGTGCC PRIMER_RIGHT_0_SEQUENCE=CCACACAGCATGATTCCCTA PRIMER_INTERNAL_0_SEQUENCE=TTTCATAATAAAAATGGGAAGTGG PRIMER_LEFT_0=283,20 PRIMER_RIGHT_0=358,20 PRIMER_INTERNAL_0=311,24 PRIMER_LEFT_0_TM=59.923 PRIMER_RIGHT_0_TM=59.522 PRIMER_INTERNAL_0_TM=58.433 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=29.167 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=8.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=0.666384 PRIMER_LEFT_1_PENALTY=0.628294 PRIMER_RIGHT_1_PENALTY=0.038090 PRIMER_INTERNAL_1_PENALTY=3.604943 PRIMER_LEFT_1_SEQUENCE=AAAGCCTGTGAGTTCCTCCC PRIMER_RIGHT_1_SEQUENCE=GCTGATAATTGCCCCTTGAA PRIMER_INTERNAL_1_SEQUENCE=AGCAATGACTACTGGGACTTTG PRIMER_LEFT_1=102,20 PRIMER_RIGHT_1=179,20 PRIMER_INTERNAL_1=122,22 PRIMER_LEFT_1_TM=60.628 PRIMER_RIGHT_1_TM=60.038 PRIMER_INTERNAL_1_TM=58.395 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.3000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=0.813808 PRIMER_LEFT_2_PENALTY=0.775718 PRIMER_RIGHT_2_PENALTY=0.038090 PRIMER_INTERNAL_2_PENALTY=0.339231 PRIMER_LEFT_2_SEQUENCE=GCACTCATGGAAAAAGCCTG PRIMER_RIGHT_2_SEQUENCE=GCTGATAATTGCCCCTTGAA PRIMER_INTERNAL_2_SEQUENCE=GAGTTCCTCCCAGCAATGAC PRIMER_LEFT_2=90,20 PRIMER_RIGHT_2=179,20 PRIMER_INTERNAL_2=111,20 PRIMER_LEFT_2_TM=60.776 PRIMER_RIGHT_2_TM=60.038 PRIMER_INTERNAL_2_TM=59.661 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=7.3000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=0.881630 PRIMER_LEFT_3_PENALTY=0.775718 PRIMER_RIGHT_3_PENALTY=0.105912 PRIMER_INTERNAL_3_PENALTY=0.339231 PRIMER_LEFT_3_SEQUENCE=GCACTCATGGAAAAAGCCTG PRIMER_RIGHT_3_SEQUENCE=TTGCCCCTTGAAAGGATATG PRIMER_INTERNAL_3_SEQUENCE=GAGTTCCTCCCAGCAATGAC PRIMER_LEFT_3=90,20 PRIMER_RIGHT_3=171,20 PRIMER_INTERNAL_3=111,20 PRIMER_LEFT_3_TM=60.776 PRIMER_RIGHT_3_TM=59.894 PRIMER_INTERNAL_3_TM=59.661 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=5.8000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=82 PRIMER_PAIR_4_PENALTY=0.928040 PRIMER_LEFT_4_PENALTY=0.775718 PRIMER_RIGHT_4_PENALTY=0.152322 PRIMER_INTERNAL_4_PENALTY=0.339231 PRIMER_LEFT_4_SEQUENCE=GCACTCATGGAAAAAGCCTG PRIMER_RIGHT_4_SEQUENCE=GCCCCTTGAAAGGATATGGT PRIMER_INTERNAL_4_SEQUENCE=GAGTTCCTCCCAGCAATGAC PRIMER_LEFT_4=90,20 PRIMER_RIGHT_4=169,20 PRIMER_INTERNAL_4=111,20 PRIMER_LEFT_4_TM=60.776 PRIMER_RIGHT_4_TM=60.152 PRIMER_INTERNAL_4_TM=59.661 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=7.8000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH1259 SEQUENCE_TEMPLATE=CTNGCANCCCTCCAGGTCGACTCTAGAGGATCCCCCTGTAAATATAACATGCTCACTAGAAATATTTTGTTTAAGGGGAAAACAAATATTGCATACCTTTTTGGGTTGTTCATTCAAAAAACCAAAGTAGTGTCTGTTTAGGGAGGGATAAGTNGATTTNCTATTAAAGGGNCTTTTCAACAAAAACAGTAGAAAGGAGACCTTTCATTTGAACAAGTCTTTATAACAAACAACACTTTGTTCTCTTAGTNNGAAAGACTATTTNCTTCTCAGGNACCAATACCTAACANCTAAAANCAGAAGATAAAGATGTATTGTTCGGAGCA SEQUENCE_INCLUDED_REGION=35,291 P3_COMMENT=CL is TGCATACCTTTTTGGGTTGT P3_COMMENT=CR is CGAACAATACATCTTTATCTTCTG P3_COMMENT=CM is CATTCAAAAAACCAAAGTAGTGTC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.912298 PRIMER_LEFT_0_PENALTY=2.515919 PRIMER_RIGHT_0_PENALTY=4.396379 PRIMER_INTERNAL_0_PENALTY=1.175168 PRIMER_LEFT_0_SEQUENCE=AAGGGGAAAACAAATATTGCA PRIMER_RIGHT_0_SEQUENCE=TCCCTCCCTAAACAGACACTACTT PRIMER_INTERNAL_0_SEQUENCE=CCTTTTTGGGTTGTTCATTCA PRIMER_LEFT_0=72,21 PRIMER_RIGHT_0=147,24 PRIMER_INTERNAL_0=95,21 PRIMER_LEFT_0_TM=58.484 PRIMER_RIGHT_0_TM=59.604 PRIMER_INTERNAL_0_TM=59.825 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=45.833 PRIMER_INTERNAL_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=5.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=7.134140 PRIMER_LEFT_1_PENALTY=2.515919 PRIMER_RIGHT_1_PENALTY=4.618221 PRIMER_INTERNAL_1_PENALTY=1.175168 PRIMER_LEFT_1_SEQUENCE=AAGGGGAAAACAAATATTGCA PRIMER_RIGHT_1_SEQUENCE=CTTATCCCTCCCTAAACAGACA PRIMER_INTERNAL_1_SEQUENCE=CCTTTTTGGGTTGTTCATTCA PRIMER_LEFT_1=72,21 PRIMER_RIGHT_1=151,22 PRIMER_INTERNAL_1=95,21 PRIMER_LEFT_1_TM=58.484 PRIMER_RIGHT_1_TM=57.382 PRIMER_INTERNAL_1_TM=59.825 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_INTERNAL_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=7.167059 PRIMER_LEFT_2_PENALTY=2.515919 PRIMER_RIGHT_2_PENALTY=4.651140 PRIMER_INTERNAL_2_PENALTY=1.175168 PRIMER_LEFT_2_SEQUENCE=AAGGGGAAAACAAATATTGCA PRIMER_RIGHT_2_SEQUENCE=TCCCTCCCTAAACAGACACTACT PRIMER_INTERNAL_2_SEQUENCE=CCTTTTTGGGTTGTTCATTCA PRIMER_LEFT_2=72,21 PRIMER_RIGHT_2=147,23 PRIMER_INTERNAL_2=95,21 PRIMER_LEFT_2_TM=58.484 PRIMER_RIGHT_2_TM=58.349 PRIMER_INTERNAL_2_TM=59.825 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=47.826 PRIMER_INTERNAL_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.8000 PRIMER_RIGHT_2_END_STABILITY=5.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=76 PRIMER_PAIR_3_PENALTY=7.170905 PRIMER_LEFT_3_PENALTY=2.515919 PRIMER_RIGHT_3_PENALTY=4.654986 PRIMER_INTERNAL_3_PENALTY=1.175168 PRIMER_LEFT_3_SEQUENCE=AAGGGGAAAACAAATATTGCA PRIMER_RIGHT_3_SEQUENCE=TCCCTCCCTAAACAGACACTAC PRIMER_INTERNAL_3_SEQUENCE=CCTTTTTGGGTTGTTCATTCA PRIMER_LEFT_3=72,21 PRIMER_RIGHT_3=147,22 PRIMER_INTERNAL_3=95,21 PRIMER_LEFT_3_TM=58.484 PRIMER_RIGHT_3_TM=57.345 PRIMER_INTERNAL_3_TM=59.825 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.8000 PRIMER_RIGHT_3_END_STABILITY=5.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=7.279827 PRIMER_LEFT_4_PENALTY=2.515919 PRIMER_RIGHT_4_PENALTY=4.763908 PRIMER_INTERNAL_4_PENALTY=1.175168 PRIMER_LEFT_4_SEQUENCE=AAGGGGAAAACAAATATTGCA PRIMER_RIGHT_4_SEQUENCE=TTATCCCTCCCTAAACAGACAC PRIMER_INTERNAL_4_SEQUENCE=CCTTTTTGGGTTGTTCATTCA PRIMER_LEFT_4=72,21 PRIMER_RIGHT_4=150,22 PRIMER_INTERNAL_4=95,21 PRIMER_LEFT_4_TM=58.484 PRIMER_RIGHT_4_TM=57.236 PRIMER_INTERNAL_4_TM=59.825 PRIMER_LEFT_4_GC_PERCENT=33.333 PRIMER_RIGHT_4_GC_PERCENT=45.455 PRIMER_INTERNAL_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.8000 PRIMER_RIGHT_4_END_STABILITY=6.1000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH1262 SEQUENCE_TEMPLATE=CTTGCATGACTCCAGGTCGACTCTAGAGGATCCCCCTGTNCTGGGCAACCTGAAAAGCATTTTCCCAAGAGAGTNGTAAGACTGACTTCTTTCTTTGTNAGGTCTCTGCTTAAATGACATTTACTCACTGAGGCCTTCCTTGATTTCCTGACATTAAAAACTCACCTACAGCCCCCTCTGCTACCTATCCTAGTTTATCTCCCTTTTTCCAGAGTATATATCACCATCTGATAATACATTTTGACTTTCTTCTTTGTTATGTCTGTTTCTGCCAACTGGAAGGCAGAAGCTCTTTTTGACCAGAGTTGTATTCTCAGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTC SEQUENCE_INCLUDED_REGION=35,281 P3_COMMENT=CL is AACCTGAAAAGCATTTTCCC P3_COMMENT=CR is AAAAAGAGCTTCTGCCTTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.761875 PRIMER_LEFT_0_PENALTY=0.045749 PRIMER_RIGHT_0_PENALTY=0.716126 PRIMER_INTERNAL_0_PENALTY=7.723451 PRIMER_LEFT_0_SEQUENCE=CCTGAAAAGCATTTTCCCAA PRIMER_RIGHT_0_SEQUENCE=AATCAAGGAAGGCCTCAGTG PRIMER_INTERNAL_0_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT PRIMER_LEFT_0=48,20 PRIMER_RIGHT_0=144,20 PRIMER_INTERNAL_0=100,25 PRIMER_LEFT_0_TM=60.046 PRIMER_RIGHT_0_TM=59.284 PRIMER_INTERNAL_0_TM=57.277 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=11.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=7.00 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=97 PRIMER_PAIR_1_PENALTY=1.170173 PRIMER_LEFT_1_PENALTY=0.454047 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_INTERNAL_1_PENALTY=7.723451 PRIMER_LEFT_1_SEQUENCE=ACCTGAAAAGCATTTTCCCA PRIMER_RIGHT_1_SEQUENCE=AATCAAGGAAGGCCTCAGTG PRIMER_INTERNAL_1_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT PRIMER_LEFT_1=47,20 PRIMER_RIGHT_1=144,20 PRIMER_INTERNAL_1=100,25 PRIMER_LEFT_1_TM=59.546 PRIMER_RIGHT_1_TM=59.284 PRIMER_INTERNAL_1_TM=57.277 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=11.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=7.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=98 PRIMER_PAIR_2_PENALTY=1.184523 PRIMER_LEFT_2_PENALTY=0.045749 PRIMER_RIGHT_2_PENALTY=1.138774 PRIMER_INTERNAL_2_PENALTY=7.723451 PRIMER_LEFT_2_SEQUENCE=CCTGAAAAGCATTTTCCCAA PRIMER_RIGHT_2_SEQUENCE=GAAATCAAGGAAGGCCTCAG PRIMER_INTERNAL_2_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT PRIMER_LEFT_2=48,20 PRIMER_RIGHT_2=146,20 PRIMER_INTERNAL_2=100,25 PRIMER_LEFT_2_TM=60.046 PRIMER_RIGHT_2_TM=58.861 PRIMER_INTERNAL_2_TM=57.277 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=36.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=11.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=7.00 PRIMER_LEFT_2_END_STABILITY=10.0000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=1.341210 PRIMER_LEFT_3_PENALTY=0.045749 PRIMER_RIGHT_3_PENALTY=1.295461 PRIMER_INTERNAL_3_PENALTY=7.723451 PRIMER_LEFT_3_SEQUENCE=CCTGAAAAGCATTTTCCCAA PRIMER_RIGHT_3_SEQUENCE=GAAATCAAGGAAGGCCTCAGT PRIMER_INTERNAL_3_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT PRIMER_LEFT_3=48,20 PRIMER_RIGHT_3=146,21 PRIMER_INTERNAL_3=100,25 PRIMER_LEFT_3_TM=60.046 PRIMER_RIGHT_3_TM=59.705 PRIMER_INTERNAL_3_TM=57.277 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=36.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=11.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=7.00 PRIMER_LEFT_3_END_STABILITY=10.0000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=1.505752 PRIMER_LEFT_4_PENALTY=0.045749 PRIMER_RIGHT_4_PENALTY=1.460004 PRIMER_INTERNAL_4_PENALTY=7.723451 PRIMER_LEFT_4_SEQUENCE=CCTGAAAAGCATTTTCCCAA PRIMER_RIGHT_4_SEQUENCE=GGAAATCAAGGAAGGCCTCA PRIMER_INTERNAL_4_SEQUENCE=GGTCTCTGCTTAAATGACATTTACT PRIMER_LEFT_4=48,20 PRIMER_RIGHT_4=147,20 PRIMER_INTERNAL_4=100,25 PRIMER_LEFT_4_TM=60.046 PRIMER_RIGHT_4_TM=61.460 PRIMER_INTERNAL_4_TM=57.277 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=36.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=11.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=7.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH1267 SEQUENCE_TEMPLATE=NTNCATNCTCCAGGTCGACTCTAGAGGATCCCCCTGGGAGCAGGTGTGTNAGGCCCCCACACACTGTAAAGCGATCCCCTTCCAAGGNGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGNTCACAATTCCACACAACATACGNGCCGGAAGCATAAAGTGTAAAGCCTGGGNNNCCTAATGNGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGANTCGGCCAACGCGCGGGGAGAGGCGNTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA SEQUENCE_INCLUDED_REGION=33,52 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH1270 SEQUENCE_TEMPLATE=CTTGCATGCNTNCAGGTCGACTCTAGAGGATCCCTCCTNGACCTNGGGCTCCGGGACTGTGTACTCGGCGCCCCAGTCGAAGCGGCGCACCGGGGCNTGTACTTGTAAAACGCGAACTGCAGCGGCGTGTCGNGCTTCTGCGNGTGCAGGCGCGTGTCGNAGCGCANACCACCCCACCCAGCGCCGNGCCGGGCCTCCCGGNACNCCATCACCCTCAGCACCGGCGCCCGGAACAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGNCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTG SEQUENCE_INCLUDED_REGION=34,201 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH1276 SEQUENCE_TEMPLATE=GTTNATNNCTCCAGGTCGACTCTAGAGGATCCCCCTGTCCAGATGGGGAGTTGGTGCATGAGTNCACAGAGGCTGGAAGNGGGNGTTGGGGAGTNGGTGGACGGTGGAACCCAGGAAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT SEQUENCE_INCLUDED_REGION=34,83 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH128 SEQUENCE_TEMPLATE=GCTTGCNTGCCTGCAGGACGTCNACTGTAGAGGATCCCCTTNAGGGTTCCNATTTAGTGCTTTACGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACANTTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATNAATTGCGTTGCGCNCANTGGCCGCTTTCCAGG SEQUENCE_INCLUDED_REGION=39,26 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH1284 SEQUENCE_TEMPLATE=CTTCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTCTCCAGCAGTNGGGAACTCATACATTACACGGCAGCCCATTTCACAGAAGGATGACTTCGGGCTCACATCTCTTAGCAACTAACTGCCTAGCCCTTCCTGGCAGAGAAGTCTCAGGTGGACTCTGTCTAAGTGGGCAACACAGGAATGCACATCAGTNCACACCCTCCTAAGGAGAAGCATCTTCCCACACCTCCATTGCAGGGCTTCTAACAATTTTNTCCCCCTCCTGGTAGGCGATGCCTGGCTCCTCTCCTAAGAAATCAAAGGGGTACNGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTNCACACAACATACGNGCCGGAAGCATAAAGTGTAAA SEQUENCE_INCLUDED_REGION=34,271 P3_COMMENT=CL is GGGCTCACATCTCTTAGCAA P3_COMMENT=CR is TGATTTCTTAGGAGAGGAGCC P3_COMMENT=CM is TAACTGCCTAGCCCTTCCTG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.211322 PRIMER_LEFT_0_PENALTY=0.014419 PRIMER_RIGHT_0_PENALTY=0.196903 PRIMER_INTERNAL_0_PENALTY=0.128434 PRIMER_LEFT_0_SEQUENCE=GGCAGAGAAGTCTCAGGTGG PRIMER_RIGHT_0_SEQUENCE=TGTGGGAAGATGCTTCTCCT PRIMER_INTERNAL_0_SEQUENCE=GCAACACAGGAATGCACATC PRIMER_LEFT_0=137,20 PRIMER_RIGHT_0=227,20 PRIMER_INTERNAL_0=172,20 PRIMER_LEFT_0_TM=59.986 PRIMER_RIGHT_0_TM=59.803 PRIMER_INTERNAL_0_TM=60.128 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=91 PRIMER_PAIR_1_PENALTY=0.235264 PRIMER_LEFT_1_PENALTY=0.014419 PRIMER_RIGHT_1_PENALTY=0.220845 PRIMER_INTERNAL_1_PENALTY=0.128434 PRIMER_LEFT_1_SEQUENCE=GGCAGAGAAGTCTCAGGTGG PRIMER_RIGHT_1_SEQUENCE=AATGGAGGTGTGGGAAGATG PRIMER_INTERNAL_1_SEQUENCE=GCAACACAGGAATGCACATC PRIMER_LEFT_1=137,20 PRIMER_RIGHT_1=235,20 PRIMER_INTERNAL_1=172,20 PRIMER_LEFT_1_TM=59.986 PRIMER_RIGHT_1_TM=59.779 PRIMER_INTERNAL_1_TM=60.128 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=0.235264 PRIMER_LEFT_2_PENALTY=0.014419 PRIMER_RIGHT_2_PENALTY=0.220845 PRIMER_INTERNAL_2_PENALTY=0.128434 PRIMER_LEFT_2_SEQUENCE=GGCAGAGAAGTCTCAGGTGG PRIMER_RIGHT_2_SEQUENCE=CAATGGAGGTGTGGGAAGAT PRIMER_INTERNAL_2_SEQUENCE=GCAACACAGGAATGCACATC PRIMER_LEFT_2=137,20 PRIMER_RIGHT_2=236,20 PRIMER_INTERNAL_2=172,20 PRIMER_LEFT_2_TM=59.986 PRIMER_RIGHT_2_TM=59.779 PRIMER_INTERNAL_2_TM=60.128 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=0.324229 PRIMER_LEFT_3_PENALTY=0.127326 PRIMER_RIGHT_3_PENALTY=0.196903 PRIMER_INTERNAL_3_PENALTY=0.128434 PRIMER_LEFT_3_SEQUENCE=CCTGGCAGAGAAGTCTCAGG PRIMER_RIGHT_3_SEQUENCE=TGTGGGAAGATGCTTCTCCT PRIMER_INTERNAL_3_SEQUENCE=GCAACACAGGAATGCACATC PRIMER_LEFT_3=134,20 PRIMER_RIGHT_3=227,20 PRIMER_INTERNAL_3=172,20 PRIMER_LEFT_3_TM=60.127 PRIMER_RIGHT_3_TM=59.803 PRIMER_INTERNAL_3_TM=60.128 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=0.341939 PRIMER_LEFT_4_PENALTY=0.128434 PRIMER_RIGHT_4_PENALTY=0.213506 PRIMER_INTERNAL_4_PENALTY=0.196903 PRIMER_LEFT_4_SEQUENCE=GCAACACAGGAATGCACATC PRIMER_RIGHT_4_SEQUENCE=GTTAGAAGCCCTGCAATGGA PRIMER_INTERNAL_4_SEQUENCE=AGGAGAAGCATCTTCCCACA PRIMER_LEFT_4=172,20 PRIMER_RIGHT_4=249,20 PRIMER_INTERNAL_4=208,20 PRIMER_LEFT_4_TM=60.128 PRIMER_RIGHT_4_TM=60.214 PRIMER_INTERNAL_4_TM=59.803 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.3000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=78 = SEQUENCE_ID=MH1290 SEQUENCE_TEMPLATE=CTTCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTTGCGCGTNGGTNGAATTGTTAATAAATTACANNCACTCTNTTCTTTGAATGTTTCTGCTCCTTTTGTATATAAGTTCCANNGGTTTCCCATGATGGATATTAATACCTACTGAAAAGAGTGACAATTGTGCTCATTGCTAAGCTGAAAAAATGTAATCCGTCTATCTGTNTTCTCTGCAGTACATAACACTAGAAAATCTAATCACCTTGAAATTGTTTGAAAGCAAGCACAAGAAAATTCATGGATGTTCACTATAGAATGAGCACAATAGAGCACCTCAGGGTNNCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCC SEQUENCE_INCLUDED_REGION=34,283 P3_COMMENT=CL is TTCTTTGAATGTTTCTGCTCC P3_COMMENT=CR is CATCCATGAATTTTCTTGTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.122709 PRIMER_LEFT_0_PENALTY=2.728489 PRIMER_RIGHT_0_PENALTY=1.394220 PRIMER_INTERNAL_0_PENALTY=2.920989 PRIMER_LEFT_0_SEQUENCE=CCTTGAAATTGTTTGAAAGCAA PRIMER_RIGHT_0_SEQUENCE=TGAGGTGCTCTATTGTGCTCA PRIMER_INTERNAL_0_SEQUENCE=GCACAAGAAAATTCATGGATG PRIMER_LEFT_0=241,22 PRIMER_RIGHT_0=316,21 PRIMER_INTERNAL_0=263,21 PRIMER_LEFT_0_TM=59.272 PRIMER_RIGHT_0_TM=59.606 PRIMER_INTERNAL_0_TM=58.079 PRIMER_LEFT_0_GC_PERCENT=31.818 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_INTERNAL_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=7.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=4.291738 PRIMER_LEFT_1_PENALTY=1.232835 PRIMER_RIGHT_1_PENALTY=3.058902 PRIMER_INTERNAL_1_PENALTY=9.021019 PRIMER_LEFT_1_SEQUENCE=TGTGCTCATTGCTAAGCTGAA PRIMER_RIGHT_1_SEQUENCE=TGCTTTCAAACAATTTCAAGG PRIMER_INTERNAL_1_SEQUENCE=TTCTCTGCAGTACATAACACTAGAAAA PRIMER_LEFT_1=162,21 PRIMER_RIGHT_1=261,21 PRIMER_INTERNAL_1=206,27 PRIMER_LEFT_1_TM=59.767 PRIMER_RIGHT_1_TM=57.941 PRIMER_INTERNAL_1_TM=57.979 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=33.333 PRIMER_INTERNAL_1_GC_PERCENT=33.333 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=4.424336 PRIMER_LEFT_2_PENALTY=3.030116 PRIMER_RIGHT_2_PENALTY=1.394220 PRIMER_INTERNAL_2_PENALTY=2.920989 PRIMER_LEFT_2_SEQUENCE=ACCTTGAAATTGTTTGAAAGCAA PRIMER_RIGHT_2_SEQUENCE=TGAGGTGCTCTATTGTGCTCA PRIMER_INTERNAL_2_SEQUENCE=GCACAAGAAAATTCATGGATG PRIMER_LEFT_2=240,23 PRIMER_RIGHT_2=316,21 PRIMER_INTERNAL_2=263,21 PRIMER_LEFT_2_TM=60.030 PRIMER_RIGHT_2_TM=59.606 PRIMER_INTERNAL_2_TM=58.079 PRIMER_LEFT_2_GC_PERCENT=30.435 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=7.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=4.445603 PRIMER_LEFT_3_PENALTY=1.232835 PRIMER_RIGHT_3_PENALTY=3.212768 PRIMER_INTERNAL_3_PENALTY=9.021019 PRIMER_LEFT_3_SEQUENCE=TGTGCTCATTGCTAAGCTGAA PRIMER_RIGHT_3_SEQUENCE=GCTTTCAAACAATTTCAAGGTG PRIMER_INTERNAL_3_SEQUENCE=TTCTCTGCAGTACATAACACTAGAAAA PRIMER_LEFT_3=162,21 PRIMER_RIGHT_3=260,22 PRIMER_INTERNAL_3=206,27 PRIMER_LEFT_3_TM=59.767 PRIMER_RIGHT_3_TM=58.787 PRIMER_INTERNAL_3_TM=57.979 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=36.364 PRIMER_INTERNAL_3_GC_PERCENT=33.333 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=4.445603 PRIMER_LEFT_4_PENALTY=1.232835 PRIMER_RIGHT_4_PENALTY=3.212768 PRIMER_INTERNAL_4_PENALTY=9.021019 PRIMER_LEFT_4_SEQUENCE=TGTGCTCATTGCTAAGCTGAA PRIMER_RIGHT_4_SEQUENCE=TGCTTTCAAACAATTTCAAGGT PRIMER_INTERNAL_4_SEQUENCE=TTCTCTGCAGTACATAACACTAGAAAA PRIMER_LEFT_4=162,21 PRIMER_RIGHT_4=261,22 PRIMER_INTERNAL_4=206,27 PRIMER_LEFT_4_TM=59.767 PRIMER_RIGHT_4_TM=58.787 PRIMER_INTERNAL_4_TM=57.979 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=31.818 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH1291 SEQUENCE_TEMPLATE=GCTTGCANNCCTCCAGGTCGACTCTAGAGGATCCCCCTAAGAAAGTNAGAATCAGGAAATATGATTTTNCTATCATGAAGGGAATTTAAATTGAGCGTGATATCTATTGCTTATAATTTTAAATTAGGCTTAATATTANGTTTTTAAAAAGTGCTAAACCACCCCTTTTAAAAACGTTTACATCTGATTCACAGAACTAAACTAAAATGTATTTGTTACAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGNGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCNAGTCGGGAAACC SEQUENCE_INCLUDED_REGION=36,185 P3_COMMENT=CL is GAAGGGAATTTAAATTGAGCG P3_COMMENT=CR is CATTTTAGTTTAGTTCTGTGAATCA P3_COMMENT=CM is GCAATAGATATCACGCTCAATTT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=13.351711 PRIMER_LEFT_0_PENALTY=5.547770 PRIMER_RIGHT_0_PENALTY=7.803941 PRIMER_INTERNAL_0_PENALTY=6.402746 PRIMER_LEFT_0_SEQUENCE=AATTGAGCGTGATATCTATTGCTT PRIMER_RIGHT_0_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG PRIMER_INTERNAL_0_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC PRIMER_LEFT_0=88,24 PRIMER_RIGHT_0=187,25 PRIMER_INTERNAL_0=139,24 PRIMER_LEFT_0_TM=58.452 PRIMER_RIGHT_0_TM=57.196 PRIMER_INTERNAL_0_TM=57.597 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=28.000 PRIMER_INTERNAL_0_GC_PERCENT=33.333 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=7.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=13.627595 PRIMER_LEFT_1_PENALTY=5.823654 PRIMER_RIGHT_1_PENALTY=7.803941 PRIMER_INTERNAL_1_PENALTY=6.402746 PRIMER_LEFT_1_SEQUENCE=ATTGAGCGTGATATCTATTGCTT PRIMER_RIGHT_1_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG PRIMER_INTERNAL_1_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC PRIMER_LEFT_1=89,23 PRIMER_RIGHT_1=187,25 PRIMER_INTERNAL_1=139,24 PRIMER_LEFT_1_TM=57.176 PRIMER_RIGHT_1_TM=57.196 PRIMER_INTERNAL_1_TM=57.597 PRIMER_LEFT_1_GC_PERCENT=34.783 PRIMER_RIGHT_1_GC_PERCENT=28.000 PRIMER_INTERNAL_1_GC_PERCENT=33.333 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=13.627595 PRIMER_LEFT_2_PENALTY=5.823654 PRIMER_RIGHT_2_PENALTY=7.803941 PRIMER_INTERNAL_2_PENALTY=6.402746 PRIMER_LEFT_2_SEQUENCE=AATTGAGCGTGATATCTATTGCT PRIMER_RIGHT_2_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG PRIMER_INTERNAL_2_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC PRIMER_LEFT_2=88,23 PRIMER_RIGHT_2=187,25 PRIMER_INTERNAL_2=139,24 PRIMER_LEFT_2_TM=57.176 PRIMER_RIGHT_2_TM=57.196 PRIMER_INTERNAL_2_TM=57.597 PRIMER_LEFT_2_GC_PERCENT=34.783 PRIMER_RIGHT_2_GC_PERCENT=28.000 PRIMER_INTERNAL_2_GC_PERCENT=33.333 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=10.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=7.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=14.293279 PRIMER_LEFT_3_PENALTY=6.489338 PRIMER_RIGHT_3_PENALTY=7.803941 PRIMER_INTERNAL_3_PENALTY=6.402746 PRIMER_LEFT_3_SEQUENCE=AATTGAGCGTGATATCTATTGCTTA PRIMER_RIGHT_3_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG PRIMER_INTERNAL_3_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC PRIMER_LEFT_3=88,25 PRIMER_RIGHT_3=187,25 PRIMER_INTERNAL_3=139,24 PRIMER_LEFT_3_TM=58.511 PRIMER_RIGHT_3_TM=57.196 PRIMER_INTERNAL_3_TM=57.597 PRIMER_LEFT_3_GC_PERCENT=32.000 PRIMER_RIGHT_3_GC_PERCENT=28.000 PRIMER_INTERNAL_3_GC_PERCENT=33.333 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=10.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=7.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.5000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=14.523720 PRIMER_LEFT_4_PENALTY=6.719779 PRIMER_RIGHT_4_PENALTY=7.803941 PRIMER_INTERNAL_4_PENALTY=6.402746 PRIMER_LEFT_4_SEQUENCE=TTGAGCGTGATATCTATTGCTTAT PRIMER_RIGHT_4_SEQUENCE=ATCAGATGTAAACGTTTTTAAAAGG PRIMER_INTERNAL_4_SEQUENCE=GTTTTTAAAAAGTGCTAAACCACC PRIMER_LEFT_4=90,24 PRIMER_RIGHT_4=187,25 PRIMER_INTERNAL_4=139,24 PRIMER_LEFT_4_TM=57.280 PRIMER_RIGHT_4_TM=57.196 PRIMER_INTERNAL_4_TM=57.597 PRIMER_LEFT_4_GC_PERCENT=33.333 PRIMER_RIGHT_4_GC_PERCENT=28.000 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=10.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=7.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=5.9000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH1292 SEQUENCE_TEMPLATE=CTNCANCCCTCCAGGTCGACTCTAGAGGATCCCTAGTNCNGANTNAGGTNGTNCCTTCAGAGCNGAGAAAGGGAGACAGGGTTACTCAGACCGGCAGTGTCACCAGTGGATGATTCCCCCNAAACACACTTCTCAACAGACTCAAGTGAGATGAAGTATACACNACAGGCAACTCGTGAATATTAACTACTTNTCTATGCTCATGCAGAATAACTTAAAATAATAAAGANTTCAACACAAATGATCAGTGACATGAATGGGAAGAGACCAATTATGATTAAACACAGCAATACTGTGAGATGAGTCAGGGGTACCGAGCTCGNATTCGTAATCATGGTCATAGCTGTTTCCNGTGTG SEQUENCE_INCLUDED_REGION=33,275 P3_COMMENT=CL is GGAGACAGGGTTACTCAGACC P3_COMMENT=CR is TTGGTCTCTTCCCATTCATG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.289927 PRIMER_LEFT_0_PENALTY=0.426379 PRIMER_RIGHT_0_PENALTY=4.863548 PRIMER_INTERNAL_0_PENALTY=0.184073 PRIMER_LEFT_0_SEQUENCE=GACAGGGTTACTCAGACCGG PRIMER_RIGHT_0_SEQUENCE=TCATCTCACTTGAGTCTGTTGAGA PRIMER_INTERNAL_0_SEQUENCE=GTCACCAGTGGATGATTCCC PRIMER_LEFT_0=74,20 PRIMER_RIGHT_0=153,24 PRIMER_INTERNAL_0=98,20 PRIMER_LEFT_0_TM=59.574 PRIMER_RIGHT_0_TM=59.136 PRIMER_INTERNAL_0_TM=60.184 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=41.667 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=7.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=11.1000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=5.564178 PRIMER_LEFT_1_PENALTY=0.426379 PRIMER_RIGHT_1_PENALTY=5.137799 PRIMER_INTERNAL_1_PENALTY=0.184073 PRIMER_LEFT_1_SEQUENCE=GACAGGGTTACTCAGACCGG PRIMER_RIGHT_1_SEQUENCE=TCTCACTTGAGTCTGTTGAGAAGTG PRIMER_INTERNAL_1_SEQUENCE=GTCACCAGTGGATGATTCCC PRIMER_LEFT_1=74,20 PRIMER_RIGHT_1=150,25 PRIMER_INTERNAL_1=98,20 PRIMER_LEFT_1_TM=59.574 PRIMER_RIGHT_1_TM=60.138 PRIMER_INTERNAL_1_TM=60.184 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=44.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=11.1000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=5.574732 PRIMER_LEFT_2_PENALTY=0.711184 PRIMER_RIGHT_2_PENALTY=4.863548 PRIMER_INTERNAL_2_PENALTY=1.747386 PRIMER_LEFT_2_SEQUENCE=GGTTACTCAGACCGGCAGTG PRIMER_RIGHT_2_SEQUENCE=TCATCTCACTTGAGTCTGTTGAGA PRIMER_INTERNAL_2_SEQUENCE=TCACCAGTGGATGATTCCC PRIMER_LEFT_2=79,20 PRIMER_RIGHT_2=153,24 PRIMER_INTERNAL_2=99,19 PRIMER_LEFT_2_TM=60.711 PRIMER_RIGHT_2_TM=59.136 PRIMER_INTERNAL_2_TM=59.253 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=41.667 PRIMER_INTERNAL_2_GC_PERCENT=52.632 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=7.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=5.626006 PRIMER_LEFT_3_PENALTY=0.184073 PRIMER_RIGHT_3_PENALTY=5.441933 PRIMER_INTERNAL_3_PENALTY=4.863548 PRIMER_LEFT_3_SEQUENCE=GTCACCAGTGGATGATTCCC PRIMER_RIGHT_3_SEQUENCE=AGTTAATATTCACGAGTTGCCTG PRIMER_INTERNAL_3_SEQUENCE=TCTCAACAGACTCAAGTGAGATGA PRIMER_LEFT_3=98,20 PRIMER_RIGHT_3=187,23 PRIMER_INTERNAL_3=130,24 PRIMER_LEFT_3_TM=60.184 PRIMER_RIGHT_3_TM=57.558 PRIMER_INTERNAL_3_TM=59.136 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=39.130 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=5.764823 PRIMER_LEFT_4_PENALTY=0.184073 PRIMER_RIGHT_4_PENALTY=5.580751 PRIMER_INTERNAL_4_PENALTY=4.863548 PRIMER_LEFT_4_SEQUENCE=GTCACCAGTGGATGATTCCC PRIMER_RIGHT_4_SEQUENCE=GTTAATATTCACGAGTTGCCTGT PRIMER_INTERNAL_4_SEQUENCE=TCTCAACAGACTCAAGTGAGATGA PRIMER_LEFT_4=98,20 PRIMER_RIGHT_4=186,23 PRIMER_INTERNAL_4=130,24 PRIMER_LEFT_4_TM=60.184 PRIMER_RIGHT_4_TM=57.419 PRIMER_INTERNAL_4_TM=59.136 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=39.130 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH1296 SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCTTTGAAAATGTNTTAAGTNCATTATTCAAACCCAAAGTNTTAATTTTTTTAAAAAACCAATTTTCCCATCTTTCAAATAAGGGGGATGGAGCAGATGATGTTCAAGATGCTCAAAATTCTTTTTGCTCCGTGAGATCTAAAACCTGACCTTGGCATCACGTAATGACCATCACAGTCAACACTGCTCACAACATATACAATGGGATGCCTGGTATGTTTTACAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGNTCACANTTCCACACA SEQUENCE_INCLUDED_REGION=34,223 P3_COMMENT=CL is GGATGGAGCAGATGATGTTC P3_COMMENT=CR is ACCAGGCATCCCATTGTATA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.133274 PRIMER_LEFT_0_PENALTY=0.106742 PRIMER_RIGHT_0_PENALTY=0.026532 PRIMER_INTERNAL_0_PENALTY=1.255615 PRIMER_LEFT_0_SEQUENCE=GGGATGGAGCAGATGATGTT PRIMER_RIGHT_0_SEQUENCE=GTGATGCCAAGGTCAGGTTT PRIMER_INTERNAL_0_SEQUENCE=CAAAATTCTTTTTGCTCCGTG PRIMER_LEFT_0=116,20 PRIMER_RIGHT_0=192,20 PRIMER_INTERNAL_0=145,21 PRIMER_LEFT_0_TM=59.893 PRIMER_RIGHT_0_TM=59.973 PRIMER_INTERNAL_0_TM=59.744 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=0.218212 PRIMER_LEFT_1_PENALTY=0.026532 PRIMER_RIGHT_1_PENALTY=0.191680 PRIMER_INTERNAL_1_PENALTY=1.674451 PRIMER_LEFT_1_SEQUENCE=AAACCTGACCTTGGCATCAC PRIMER_RIGHT_1_SEQUENCE=AAAACATACCAGGCATCCCA PRIMER_INTERNAL_1_SEQUENCE=CCATCACAGTCAACACTGCTC PRIMER_LEFT_1=173,20 PRIMER_RIGHT_1=253,20 PRIMER_INTERNAL_1=200,21 PRIMER_LEFT_1_TM=59.973 PRIMER_RIGHT_1_TM=60.192 PRIMER_INTERNAL_1_TM=59.326 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.3000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=81 PRIMER_PAIR_2_PENALTY=0.276511 PRIMER_LEFT_2_PENALTY=0.249979 PRIMER_RIGHT_2_PENALTY=0.026532 PRIMER_INTERNAL_2_PENALTY=1.255615 PRIMER_LEFT_2_SEQUENCE=ATAAGGGGGATGGAGCAGAT PRIMER_RIGHT_2_SEQUENCE=GTGATGCCAAGGTCAGGTTT PRIMER_INTERNAL_2_SEQUENCE=CAAAATTCTTTTTGCTCCGTG PRIMER_LEFT_2=110,20 PRIMER_RIGHT_2=192,20 PRIMER_INTERNAL_2=145,21 PRIMER_LEFT_2_TM=59.750 PRIMER_RIGHT_2_TM=59.973 PRIMER_INTERNAL_2_TM=59.744 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=0.305015 PRIMER_LEFT_3_PENALTY=0.278483 PRIMER_RIGHT_3_PENALTY=0.026532 PRIMER_INTERNAL_3_PENALTY=1.255615 PRIMER_LEFT_3_SEQUENCE=AAATAAGGGGGATGGAGCAG PRIMER_RIGHT_3_SEQUENCE=GTGATGCCAAGGTCAGGTTT PRIMER_INTERNAL_3_SEQUENCE=CAAAATTCTTTTTGCTCCGTG PRIMER_LEFT_3=108,20 PRIMER_RIGHT_3=192,20 PRIMER_INTERNAL_3=145,21 PRIMER_LEFT_3_TM=60.278 PRIMER_RIGHT_3_TM=59.973 PRIMER_INTERNAL_3_TM=59.744 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=85 PRIMER_PAIR_4_PENALTY=0.391116 PRIMER_LEFT_4_PENALTY=0.106742 PRIMER_RIGHT_4_PENALTY=0.284374 PRIMER_INTERNAL_4_PENALTY=1.255615 PRIMER_LEFT_4_SEQUENCE=GGGATGGAGCAGATGATGTT PRIMER_RIGHT_4_SEQUENCE=TTACGTGATGCCAAGGTCAG PRIMER_INTERNAL_4_SEQUENCE=CAAAATTCTTTTTGCTCCGTG PRIMER_LEFT_4=116,20 PRIMER_RIGHT_4=196,20 PRIMER_INTERNAL_4=145,21 PRIMER_LEFT_4_TM=59.893 PRIMER_RIGHT_4_TM=59.716 PRIMER_INTERNAL_4_TM=59.744 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=81 = SEQUENCE_ID=MH1304 SEQUENCE_TEMPLATE=GNGGCATGNNCAGGTCGACTCTAGAGGATCCCCTGTGTGACAGACCCTAAAGTGGGGAACCAGTNTAAGCCATACTCAGATTNNCAAACCACCGAAACAGTGAAATATTCAGTGTATGTNATTTTAAACTGCTGATTTTAGTGGTGACTTGTTATGCAACANTAGATAACTAATACATATATAANTGGAAACGCATACACATATGCCCTTTATGTTTGGCATACTTTTCTGGAGANTTGGAAAGGTTAAATAGGAGTTTACACCATAAACATGTAAAANNNNTGCAANTTGAATGTTAGANNTTNTNCTCAGTCCTTCANCAGGGTA SEQUENCE_INCLUDED_REGION=33,294 P3_COMMENT=CL is ACAGACCCTAAAGTGGGGAA P3_COMMENT=CR is TTTACATGTTTATGGTGTAAACTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.505285 PRIMER_LEFT_0_PENALTY=3.785641 PRIMER_RIGHT_0_PENALTY=2.719644 PRIMER_INTERNAL_0_PENALTY=1.231281 PRIMER_LEFT_0_SEQUENCE=AGTGGTGACTTGTTATGCAACA PRIMER_RIGHT_0_SEQUENCE=TCCAGAAAAGTATGCCAAACA PRIMER_INTERNAL_0_SEQUENCE=GAAACGCATACACATATGCCC PRIMER_LEFT_0=139,22 PRIMER_RIGHT_0=232,21 PRIMER_INTERNAL_0=187,21 PRIMER_LEFT_0_TM=58.214 PRIMER_RIGHT_0_TM=58.280 PRIMER_INTERNAL_0_TM=60.231 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_INTERNAL_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=94 PRIMER_PAIR_1_PENALTY=6.518732 PRIMER_LEFT_1_PENALTY=3.785641 PRIMER_RIGHT_1_PENALTY=2.733091 PRIMER_INTERNAL_1_PENALTY=1.231281 PRIMER_LEFT_1_SEQUENCE=AGTGGTGACTTGTTATGCAACA PRIMER_RIGHT_1_SEQUENCE=CTCCAGAAAAGTATGCCAAACA PRIMER_INTERNAL_1_SEQUENCE=GAAACGCATACACATATGCCC PRIMER_LEFT_1=139,22 PRIMER_RIGHT_1=233,22 PRIMER_INTERNAL_1=187,21 PRIMER_LEFT_1_TM=58.214 PRIMER_RIGHT_1_TM=59.267 PRIMER_INTERNAL_1_TM=60.231 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_INTERNAL_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=95 PRIMER_PAIR_2_PENALTY=6.619916 PRIMER_LEFT_2_PENALTY=3.900272 PRIMER_RIGHT_2_PENALTY=2.719644 PRIMER_INTERNAL_2_PENALTY=1.231281 PRIMER_LEFT_2_SEQUENCE=GTGGTGACTTGTTATGCAACA PRIMER_RIGHT_2_SEQUENCE=TCCAGAAAAGTATGCCAAACA PRIMER_INTERNAL_2_SEQUENCE=GAAACGCATACACATATGCCC PRIMER_LEFT_2=140,21 PRIMER_RIGHT_2=232,21 PRIMER_INTERNAL_2=187,21 PRIMER_LEFT_2_TM=57.100 PRIMER_RIGHT_2_TM=58.280 PRIMER_INTERNAL_2_TM=60.231 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=38.095 PRIMER_INTERNAL_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=93 PRIMER_PAIR_3_PENALTY=6.633363 PRIMER_LEFT_3_PENALTY=3.900272 PRIMER_RIGHT_3_PENALTY=2.733091 PRIMER_INTERNAL_3_PENALTY=1.231281 PRIMER_LEFT_3_SEQUENCE=GTGGTGACTTGTTATGCAACA PRIMER_RIGHT_3_SEQUENCE=CTCCAGAAAAGTATGCCAAACA PRIMER_INTERNAL_3_SEQUENCE=GAAACGCATACACATATGCCC PRIMER_LEFT_3=140,21 PRIMER_RIGHT_3=233,22 PRIMER_INTERNAL_3=187,21 PRIMER_LEFT_3_TM=57.100 PRIMER_RIGHT_3_TM=59.267 PRIMER_INTERNAL_3_TM=60.231 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=40.909 PRIMER_INTERNAL_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=7.101617 PRIMER_LEFT_4_PENALTY=4.381973 PRIMER_RIGHT_4_PENALTY=2.719644 PRIMER_INTERNAL_4_PENALTY=1.231281 PRIMER_LEFT_4_SEQUENCE=TTAGTGGTGACTTGTTATGCAACA PRIMER_RIGHT_4_SEQUENCE=TCCAGAAAAGTATGCCAAACA PRIMER_INTERNAL_4_SEQUENCE=GAAACGCATACACATATGCCC PRIMER_LEFT_4=137,24 PRIMER_RIGHT_4=232,21 PRIMER_INTERNAL_4=187,21 PRIMER_LEFT_4_TM=59.618 PRIMER_RIGHT_4_TM=58.280 PRIMER_INTERNAL_4_TM=60.231 PRIMER_LEFT_4_GC_PERCENT=37.500 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_INTERNAL_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=96 = SEQUENCE_ID=MH1306 SEQUENCE_TEMPLATE=NTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGTTTCTGTACCTCACTTCTGATTTCTGTATGTCACTTTCCTTTTGTTGTNTATAAATTTGTTCTGACCACGTGGCATCCCTGGNGTNNCTCTGNATCTGCTGTGATTCTGGAGACTGCCAGATCTGCTAATCATTTTTTTTTCTTGGTCCGTTAAACTCTGTTAAATTTAATTTGTCCGAAGTTTTCTTTTAACAGGGGAAAGCATCGTATTCATAATGGCAGTAAGAACCCGATATAATTCCCTAGAAAAAAGTTATTCTCCTTTAACCCAGTAACCCTACTTCTATGACTCTATTTATGGGNAAAATCCAAAGTA SEQUENCE_INCLUDED_REGION=35,318 P3_COMMENT=CL is TCTGATTTCTGTATGTCACTTTCC P3_COMMENT=CR is TCGGGTTCTTACTGCCATTA P3_COMMENT=CM is TATAAATTTGTTCTGACCACGTGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.155134 PRIMER_LEFT_0_PENALTY=1.087947 PRIMER_RIGHT_0_PENALTY=0.067187 PRIMER_INTERNAL_0_PENALTY=1.915462 PRIMER_LEFT_0_SEQUENCE=TGGAGACTGCCAGATCTGC PRIMER_RIGHT_0_SEQUENCE=CGATGCTTTCCCCTGTTAAA PRIMER_INTERNAL_0_SEQUENCE=TCATTTTTTTTTCTTGGTCCG PRIMER_LEFT_0=144,19 PRIMER_RIGHT_0=243,20 PRIMER_INTERNAL_0=166,21 PRIMER_LEFT_0_TM=60.088 PRIMER_RIGHT_0_TM=60.067 PRIMER_INTERNAL_0_TM=59.085 PRIMER_LEFT_0_GC_PERCENT=57.895 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=33.333 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=7.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=1.192410 PRIMER_LEFT_1_PENALTY=1.125223 PRIMER_RIGHT_1_PENALTY=0.067187 PRIMER_INTERNAL_1_PENALTY=1.915462 PRIMER_LEFT_1_SEQUENCE=TGGAGACTGCCAGATCTGCT PRIMER_RIGHT_1_SEQUENCE=CGATGCTTTCCCCTGTTAAA PRIMER_INTERNAL_1_SEQUENCE=TCATTTTTTTTTCTTGGTCCG PRIMER_LEFT_1=144,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=166,21 PRIMER_LEFT_1_TM=61.125 PRIMER_RIGHT_1_TM=60.067 PRIMER_INTERNAL_1_TM=59.085 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=33.333 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=1.478710 PRIMER_LEFT_2_PENALTY=1.411523 PRIMER_RIGHT_2_PENALTY=0.067187 PRIMER_INTERNAL_2_PENALTY=1.915462 PRIMER_LEFT_2_SEQUENCE=GGAGACTGCCAGATCTGCTAA PRIMER_RIGHT_2_SEQUENCE=CGATGCTTTCCCCTGTTAAA PRIMER_INTERNAL_2_SEQUENCE=TCATTTTTTTTTCTTGGTCCG PRIMER_LEFT_2=145,21 PRIMER_RIGHT_2=243,20 PRIMER_INTERNAL_2=166,21 PRIMER_LEFT_2_TM=59.588 PRIMER_RIGHT_2_TM=60.067 PRIMER_INTERNAL_2_TM=59.085 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=33.333 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.5000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=1.842580 PRIMER_LEFT_3_PENALTY=1.411523 PRIMER_RIGHT_3_PENALTY=0.431057 PRIMER_INTERNAL_3_PENALTY=1.915462 PRIMER_LEFT_3_SEQUENCE=GGAGACTGCCAGATCTGCTAA PRIMER_RIGHT_3_SEQUENCE=ACGATGCTTTCCCCTGTTAA PRIMER_INTERNAL_3_SEQUENCE=TCATTTTTTTTTCTTGGTCCG PRIMER_LEFT_3=145,21 PRIMER_RIGHT_3=244,20 PRIMER_INTERNAL_3=166,21 PRIMER_LEFT_3_TM=59.588 PRIMER_RIGHT_3_TM=59.569 PRIMER_INTERNAL_3_TM=59.085 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=33.333 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.5000 PRIMER_RIGHT_3_END_STABILITY=6.0000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=1.938080 PRIMER_LEFT_4_PENALTY=1.870893 PRIMER_RIGHT_4_PENALTY=0.067187 PRIMER_INTERNAL_4_PENALTY=1.915462 PRIMER_LEFT_4_SEQUENCE=GGAGACTGCCAGATCTGCTA PRIMER_RIGHT_4_SEQUENCE=CGATGCTTTCCCCTGTTAAA PRIMER_INTERNAL_4_SEQUENCE=TCATTTTTTTTTCTTGGTCCG PRIMER_LEFT_4=145,20 PRIMER_RIGHT_4=243,20 PRIMER_INTERNAL_4=166,21 PRIMER_LEFT_4_TM=58.129 PRIMER_RIGHT_4_TM=60.067 PRIMER_INTERNAL_4_TM=59.085 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.5000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = SEQUENCE_ID=MH1307 SEQUENCE_TEMPLATE=CTTCATTCTCCAGGTCGACTCTAGAGGATCCCCTGTCTAACACAGCCTATCGATATAGTNTNNTAGATCTATTTTCTGCAGGACTATGCATAAAATCCTGGGAGAGTTCATAGAAAGTNCATCTACCTAAGGCAGAAGAACAGGCAGCACCTTCAAGTNCTTCTGTTACATGGGCAATAGGATGNAGAAGTGAGCAGCATGAGCAAAGTTATTGAAGTATGCTAGAAAATAATGCTCAAGGTCTTAAGCAATTTAATAGGTAAAGGTTAGNGTNCATGNGGGTGGTAGAAGGTCAGANTAGAGTNNTAGANGNGGCATGGTAAANGNCTGGAGCAGTGG SEQUENCE_INCLUDED_REGION=33,306 P3_COMMENT=CL is ATCTATTTTCTGCAGGACTATGC P3_COMMENT=CR is CCTTTACCTATTAAATTGCTTAAGA P3_COMMENT=CM is TAAAATCCTGGGAGAGTTCATAGA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.257145 PRIMER_LEFT_0_PENALTY=0.003322 PRIMER_RIGHT_0_PENALTY=0.253823 PRIMER_INTERNAL_0_PENALTY=2.526059 PRIMER_LEFT_0_SEQUENCE=GAAGAACAGGCAGCACCTTC PRIMER_RIGHT_0_SEQUENCE=AACTTTGCTCATGCTGCTCA PRIMER_INTERNAL_0_SEQUENCE=TTCTGTTACATGGGCAATAGGA PRIMER_LEFT_0=134,20 PRIMER_RIGHT_0=209,20 PRIMER_INTERNAL_0=160,22 PRIMER_LEFT_0_TM=59.997 PRIMER_RIGHT_0_TM=59.746 PRIMER_INTERNAL_0_TM=59.474 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=0.808009 PRIMER_LEFT_1_PENALTY=0.003322 PRIMER_RIGHT_1_PENALTY=0.804687 PRIMER_INTERNAL_1_PENALTY=2.526059 PRIMER_LEFT_1_SEQUENCE=GAAGAACAGGCAGCACCTTC PRIMER_RIGHT_1_SEQUENCE=ACTTTGCTCATGCTGCTCAC PRIMER_INTERNAL_1_SEQUENCE=TTCTGTTACATGGGCAATAGGA PRIMER_LEFT_1=134,20 PRIMER_RIGHT_1=208,20 PRIMER_INTERNAL_1=160,22 PRIMER_LEFT_1_TM=59.997 PRIMER_RIGHT_1_TM=59.195 PRIMER_INTERNAL_1_TM=59.474 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=0.842790 PRIMER_LEFT_2_PENALTY=0.588967 PRIMER_RIGHT_2_PENALTY=0.253823 PRIMER_INTERNAL_2_PENALTY=2.526059 PRIMER_LEFT_2_SEQUENCE=CAGAAGAACAGGCAGCACCT PRIMER_RIGHT_2_SEQUENCE=TTTGCTCATGCTGCTCACTT PRIMER_INTERNAL_2_SEQUENCE=TTCTGTTACATGGGCAATAGGA PRIMER_LEFT_2=132,20 PRIMER_RIGHT_2=206,20 PRIMER_INTERNAL_2=160,22 PRIMER_LEFT_2_TM=60.589 PRIMER_RIGHT_2_TM=59.746 PRIMER_INTERNAL_2_TM=59.474 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=0.842790 PRIMER_LEFT_3_PENALTY=0.588967 PRIMER_RIGHT_3_PENALTY=0.253823 PRIMER_INTERNAL_3_PENALTY=2.526059 PRIMER_LEFT_3_SEQUENCE=CAGAAGAACAGGCAGCACCT PRIMER_RIGHT_3_SEQUENCE=AACTTTGCTCATGCTGCTCA PRIMER_INTERNAL_3_SEQUENCE=TTCTGTTACATGGGCAATAGGA PRIMER_LEFT_3=132,20 PRIMER_RIGHT_3=209,20 PRIMER_INTERNAL_3=160,22 PRIMER_LEFT_3_TM=60.589 PRIMER_RIGHT_3_TM=59.746 PRIMER_INTERNAL_3_TM=59.474 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=78 PRIMER_PAIR_4_PENALTY=1.036230 PRIMER_LEFT_4_PENALTY=0.923358 PRIMER_RIGHT_4_PENALTY=0.112872 PRIMER_INTERNAL_4_PENALTY=2.526059 PRIMER_LEFT_4_SEQUENCE=CCTAAGGCAGAAGAACAGGC PRIMER_RIGHT_4_SEQUENCE=TGCTCATGCTGCTCACTTCT PRIMER_INTERNAL_4_SEQUENCE=TTCTGTTACATGGGCAATAGGA PRIMER_LEFT_4=125,20 PRIMER_RIGHT_4=204,20 PRIMER_INTERNAL_4=160,22 PRIMER_LEFT_4_TM=59.077 PRIMER_RIGHT_4_TM=59.887 PRIMER_INTERNAL_4_TM=59.474 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH131 SEQUENCE_TEMPLATE=TCATGCCTGCAGGTCGACTCTAGAGGATCCCCTAATAATAATTTAGTAGGATTTTTTTTTTCTTTGCCTTATGTAGACTCTGTNCTTCCTTGCAGCCCATTGAGAGCAATGATCACCATGACTGTNCCCTCACATGCAGCAGCAATGATTGTCCAAGATATACATGTTCACAGAACAAACTTTTATCATACAAGTTTTCTTTTTCTGAGAAATGTGAAGTTCCAGAATACCCTTATTTTTTTAATTTNCT SEQUENCE_INCLUDED_REGION=31,219 P3_COMMENT=CL is TGAGAGCAATGATCACCATG P3_COMMENT=CR is AGGGTATTCTGGAACTTCACA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.439719 PRIMER_LEFT_0_PENALTY=2.343918 PRIMER_RIGHT_0_PENALTY=1.095801 PRIMER_INTERNAL_0_PENALTY=1.895060 PRIMER_LEFT_0_SEQUENCE=CTTCCTTGCAGCCCATTG PRIMER_RIGHT_0_SEQUENCE=ATCTTGGACAATCATTGCTGC PRIMER_INTERNAL_0_SEQUENCE=GAGCAATGATCACCATGACTG PRIMER_LEFT_0=84,18 PRIMER_RIGHT_0=158,21 PRIMER_INTERNAL_0=103,21 PRIMER_LEFT_0_TM=60.344 PRIMER_RIGHT_0_TM=60.096 PRIMER_INTERNAL_0_TM=59.105 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_INTERNAL_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=4.194322 PRIMER_LEFT_1_PENALTY=0.163550 PRIMER_RIGHT_1_PENALTY=4.030772 PRIMER_INTERNAL_1_PENALTY=6.469560 PRIMER_LEFT_1_SEQUENCE=ATGCAGCAGCAATGATTGTC PRIMER_RIGHT_1_SEQUENCE=TCTGGAACTTCACATTTCTCAGA PRIMER_INTERNAL_1_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA PRIMER_LEFT_1=133,20 PRIMER_RIGHT_1=225,23 PRIMER_INTERNAL_1=162,26 PRIMER_LEFT_1_TM=59.836 PRIMER_RIGHT_1_TM=58.969 PRIMER_INTERNAL_1_TM=59.530 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=39.130 PRIMER_INTERNAL_1_GC_PERCENT=30.769 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=93 PRIMER_PAIR_2_PENALTY=4.225370 PRIMER_LEFT_2_PENALTY=0.194598 PRIMER_RIGHT_2_PENALTY=4.030772 PRIMER_INTERNAL_2_PENALTY=6.469560 PRIMER_LEFT_2_SEQUENCE=GCAGCAATGATTGTCCAAGA PRIMER_RIGHT_2_SEQUENCE=TCTGGAACTTCACATTTCTCAGA PRIMER_INTERNAL_2_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA PRIMER_LEFT_2=138,20 PRIMER_RIGHT_2=225,23 PRIMER_INTERNAL_2=162,26 PRIMER_LEFT_2_TM=59.805 PRIMER_RIGHT_2_TM=58.969 PRIMER_INTERNAL_2_TM=59.530 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=39.130 PRIMER_INTERNAL_2_GC_PERCENT=30.769 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=7.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=88 PRIMER_PAIR_3_PENALTY=4.278266 PRIMER_LEFT_3_PENALTY=0.163550 PRIMER_RIGHT_3_PENALTY=4.114715 PRIMER_INTERNAL_3_PENALTY=6.469560 PRIMER_LEFT_3_SEQUENCE=ATGCAGCAGCAATGATTGTC PRIMER_RIGHT_3_SEQUENCE=GGGTATTCTGGAACTTCACATTTC PRIMER_INTERNAL_3_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA PRIMER_LEFT_3=133,20 PRIMER_RIGHT_3=231,24 PRIMER_INTERNAL_3=162,26 PRIMER_LEFT_3_TM=59.836 PRIMER_RIGHT_3_TM=60.115 PRIMER_INTERNAL_3_TM=59.530 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=41.667 PRIMER_INTERNAL_3_GC_PERCENT=30.769 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=4.305833 PRIMER_LEFT_4_PENALTY=0.163550 PRIMER_RIGHT_4_PENALTY=4.142283 PRIMER_INTERNAL_4_PENALTY=6.469560 PRIMER_LEFT_4_SEQUENCE=ATGCAGCAGCAATGATTGTC PRIMER_RIGHT_4_SEQUENCE=TGGAACTTCACATTTCTCAGAAAA PRIMER_INTERNAL_4_SEQUENCE=CATGTTCACAGAACAAACTTTTATCA PRIMER_LEFT_4=133,20 PRIMER_RIGHT_4=223,24 PRIMER_INTERNAL_4=162,26 PRIMER_LEFT_4_TM=59.836 PRIMER_RIGHT_4_TM=60.142 PRIMER_INTERNAL_4_TM=59.530 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=33.333 PRIMER_INTERNAL_4_GC_PERCENT=30.769 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=9.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=7.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.3000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH1311 SEQUENCE_TEMPLATE=TCTTGCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTCTGGTNNNNGAGNNNNNATATATATTGAATAATAGGTTACACCTATATATTACATCTATANTATGCCAAGAAAACCTTCAGAAGTNATACTAGAGTNNATGTGGTCTGCAGCCTTGAGCAGAAAAATTGCCTGTTAATCACTGATAGTTGAATATAAGNTTAAGATACATGCATACACAATTATACTGCCAGTTTCACATATGAGCCTCTTTGTGTNCAATANAGATTGTGATGAAAATGCACAGGGTACCGAGCTCGAATTNGTAATCATGGTCATAGCTGTTTC SEQUENCE_INCLUDED_REGION=36,246 P3_COMMENT=CL is TGCCAAGAAAACCTTCAGAA P3_COMMENT=CR is GGCTCATATGTGAAACTGGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.229880 PRIMER_LEFT_0_PENALTY=0.402858 PRIMER_RIGHT_0_PENALTY=1.827022 PRIMER_INTERNAL_0_PENALTY=5.069319 PRIMER_LEFT_0_SEQUENCE=GCAGCCTTGAGCAGAAAAAT PRIMER_RIGHT_0_SEQUENCE=GGCTCATATGTGAAACTGGC PRIMER_INTERNAL_0_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA PRIMER_LEFT_0=145,20 PRIMER_RIGHT_0=244,20 PRIMER_INTERNAL_0=165,25 PRIMER_LEFT_0_TM=59.597 PRIMER_RIGHT_0_TM=58.173 PRIMER_INTERNAL_0_TM=60.069 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=2.450146 PRIMER_LEFT_1_PENALTY=0.402858 PRIMER_RIGHT_1_PENALTY=2.047287 PRIMER_INTERNAL_1_PENALTY=5.069319 PRIMER_LEFT_1_SEQUENCE=GCAGCCTTGAGCAGAAAAAT PRIMER_RIGHT_1_SEQUENCE=GGCTCATATGTGAAACTGGCA PRIMER_INTERNAL_1_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA PRIMER_LEFT_1=145,20 PRIMER_RIGHT_1=244,21 PRIMER_INTERNAL_1=165,25 PRIMER_LEFT_1_TM=59.597 PRIMER_RIGHT_1_TM=61.047 PRIMER_INTERNAL_1_TM=60.069 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_INTERNAL_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=3.009711 PRIMER_LEFT_2_PENALTY=0.402858 PRIMER_RIGHT_2_PENALTY=2.606853 PRIMER_INTERNAL_2_PENALTY=5.069319 PRIMER_LEFT_2_SEQUENCE=GCAGCCTTGAGCAGAAAAAT PRIMER_RIGHT_2_SEQUENCE=GCTCATATGTGAAACTGGCAG PRIMER_INTERNAL_2_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA PRIMER_LEFT_2=145,20 PRIMER_RIGHT_2=243,21 PRIMER_INTERNAL_2=165,25 PRIMER_LEFT_2_TM=59.597 PRIMER_RIGHT_2_TM=58.393 PRIMER_INTERNAL_2_TM=60.069 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=36.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=3.125885 PRIMER_LEFT_3_PENALTY=0.402858 PRIMER_RIGHT_3_PENALTY=2.723026 PRIMER_INTERNAL_3_PENALTY=5.069319 PRIMER_LEFT_3_SEQUENCE=GCAGCCTTGAGCAGAAAAAT PRIMER_RIGHT_3_SEQUENCE=GCTCATATGTGAAACTGGCA PRIMER_INTERNAL_3_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA PRIMER_LEFT_3=145,20 PRIMER_RIGHT_3=243,20 PRIMER_INTERNAL_3=165,25 PRIMER_LEFT_3_TM=59.597 PRIMER_RIGHT_3_TM=57.277 PRIMER_INTERNAL_3_TM=60.069 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=36.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=3.132696 PRIMER_LEFT_4_PENALTY=0.402858 PRIMER_RIGHT_4_PENALTY=2.729838 PRIMER_INTERNAL_4_PENALTY=5.069319 PRIMER_LEFT_4_SEQUENCE=GCAGCCTTGAGCAGAAAAAT PRIMER_RIGHT_4_SEQUENCE=GCTCATATGTGAAACTGGCAGT PRIMER_INTERNAL_4_SEQUENCE=TGCCTGTTAATCACTGATAGTTGAA PRIMER_LEFT_4=145,20 PRIMER_RIGHT_4=243,22 PRIMER_INTERNAL_4=165,25 PRIMER_LEFT_4_TM=59.597 PRIMER_RIGHT_4_TM=59.270 PRIMER_INTERNAL_4_TM=60.069 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.455 PRIMER_INTERNAL_4_GC_PERCENT=36.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=7.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = SEQUENCE_ID=MH1313 SEQUENCE_TEMPLATE=GNTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTCCTCAGCCAGANACCTGACTTTCTCTCCCTCACAACCACCCATGACCAAGCCTGTCAATTTCACCTCCTATTAATAATTTCCCCTGAACCAGTCCACTCCCCTCCATCTCTATCACCACCATCCTGGGGGAGGAGACCACTACCATCTCAAACCTATGTTACACACAGTCTTCCTAACTGAAATGCTGAGCCTCTTCTAAAGCACTAGAACTTTCAAAGGCTCAGTCCTACCTTTCTCACCCTCCTGCTCAAAACCTACTCCCACTGCCATTTGTCTAAACAAAACTCTTTTATGTGGCATGAAAGGCATTTCTTGATCTATCTCC SEQUENCE_INCLUDED_REGION=36,328 P3_COMMENT=CL is CTTTCTCTCCCTCACAACCA P3_COMMENT=CR is ATAGATCAAGAAATGCCTTTCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.208764 PRIMER_LEFT_0_PENALTY=0.088170 PRIMER_RIGHT_0_PENALTY=0.120594 PRIMER_INTERNAL_0_PENALTY=0.168650 PRIMER_LEFT_0_SEQUENCE=TTCTCTCCCTCACAACCACC PRIMER_RIGHT_0_SEQUENCE=TGGTGATAGAGATGGAGGGG PRIMER_INTERNAL_0_SEQUENCE=AATTTCCCCTGAACCAGTCC PRIMER_LEFT_0=58,20 PRIMER_RIGHT_0=155,20 PRIMER_INTERNAL_0=113,20 PRIMER_LEFT_0_TM=60.088 PRIMER_RIGHT_0_TM=59.879 PRIMER_INTERNAL_0_TM=60.169 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=10.9000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=7.00 PRIMER_PAIR_0_PRODUCT_SIZE=98 PRIMER_PAIR_1_PENALTY=0.237022 PRIMER_LEFT_1_PENALTY=0.156061 PRIMER_RIGHT_1_PENALTY=0.080962 PRIMER_INTERNAL_1_PENALTY=0.420772 PRIMER_LEFT_1_SEQUENCE=CTCACCCTCCTGCTCAAAAC PRIMER_RIGHT_1_SEQUENCE=AGAAATGCCTTTCATGCCAC PRIMER_INTERNAL_1_SEQUENCE=ACTCCCACTGCCATTTGTCT PRIMER_LEFT_1=273,20 PRIMER_RIGHT_1=351,20 PRIMER_INTERNAL_1=295,20 PRIMER_LEFT_1_TM=59.844 PRIMER_RIGHT_1_TM=60.081 PRIMER_INTERNAL_1_TM=59.579 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=79 PRIMER_PAIR_2_PENALTY=0.256820 PRIMER_LEFT_2_PENALTY=0.088170 PRIMER_RIGHT_2_PENALTY=0.168650 PRIMER_INTERNAL_2_PENALTY=0.495173 PRIMER_LEFT_2_SEQUENCE=TTCTCTCCCTCACAACCACC PRIMER_RIGHT_2_SEQUENCE=GGACTGGTTCAGGGGAAATT PRIMER_INTERNAL_2_SEQUENCE=CCAAGCCTGTCAATTTCACC PRIMER_LEFT_2=58,20 PRIMER_RIGHT_2=132,20 PRIMER_INTERNAL_2=83,20 PRIMER_LEFT_2_TM=60.088 PRIMER_RIGHT_2_TM=60.169 PRIMER_INTERNAL_2_TM=60.495 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=7.2000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=0.325634 PRIMER_LEFT_3_PENALTY=0.252279 PRIMER_RIGHT_3_PENALTY=0.073355 PRIMER_INTERNAL_3_PENALTY=0.407941 PRIMER_LEFT_3_SEQUENCE=ACCTTTCTCACCCTCCTGCT PRIMER_RIGHT_3_SEQUENCE=TGCCTTTCATGCCACATAAA PRIMER_INTERNAL_3_SEQUENCE=AAAACCTACTCCCACTGCCA PRIMER_LEFT_3=267,20 PRIMER_RIGHT_3=346,20 PRIMER_INTERNAL_3=288,20 PRIMER_LEFT_3_TM=60.252 PRIMER_RIGHT_3_TM=60.073 PRIMER_INTERNAL_3_TM=59.592 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=0.333241 PRIMER_LEFT_4_PENALTY=0.252279 PRIMER_RIGHT_4_PENALTY=0.080962 PRIMER_INTERNAL_4_PENALTY=0.407941 PRIMER_LEFT_4_SEQUENCE=ACCTTTCTCACCCTCCTGCT PRIMER_RIGHT_4_SEQUENCE=AGAAATGCCTTTCATGCCAC PRIMER_INTERNAL_4_SEQUENCE=AAAACCTACTCCCACTGCCA PRIMER_LEFT_4=267,20 PRIMER_RIGHT_4=351,20 PRIMER_INTERNAL_4=288,20 PRIMER_LEFT_4_TM=60.252 PRIMER_RIGHT_4_TM=60.081 PRIMER_INTERNAL_4_TM=59.592 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=85 = SEQUENCE_ID=MH1314 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGGACATGCAGACAGTGTGTTCATTCCTTTTCATCATTGCCCTTTTAAGTCTGTTTTATCAAATACTTCTTTCCTGCCCTGGGATAAAATCTTGGGCTTTTCCAACTACTCTCAGGTATTAATGAATAGTGTGAATGAATAATCTAATTTTCCAAGCATTGAAAAGTTTAATATCCGTATCAGTTAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGC SEQUENCE_INCLUDED_REGION=36,187 P3_COMMENT=CL is CATTCCTTTTCATCATTGCC P3_COMMENT=CR is ATACGGATATTAAACTTTTCAATGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.289165 PRIMER_LEFT_0_PENALTY=1.183253 PRIMER_RIGHT_0_PENALTY=0.105912 PRIMER_INTERNAL_0_PENALTY=0.495347 PRIMER_LEFT_0_SEQUENCE=CTGGACATGCAGACAGTGTG PRIMER_RIGHT_0_SEQUENCE=CAAGATTTTATCCCAGGGCA PRIMER_INTERNAL_0_SEQUENCE=TCCTTTTCATCATTGCCCTT PRIMER_LEFT_0=36,20 PRIMER_RIGHT_0=130,20 PRIMER_INTERNAL_0=61,20 PRIMER_LEFT_0_TM=58.817 PRIMER_RIGHT_0_TM=59.894 PRIMER_INTERNAL_0_TM=59.505 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=11.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=95 PRIMER_PAIR_1_PENALTY=1.307131 PRIMER_LEFT_1_PENALTY=1.201218 PRIMER_RIGHT_1_PENALTY=0.105912 PRIMER_INTERNAL_1_PENALTY=0.495347 PRIMER_LEFT_1_SEQUENCE=CTGGACATGCAGACAGTGTGT PRIMER_RIGHT_1_SEQUENCE=CAAGATTTTATCCCAGGGCA PRIMER_INTERNAL_1_SEQUENCE=TCCTTTTCATCATTGCCCTT PRIMER_LEFT_1=36,21 PRIMER_RIGHT_1=130,20 PRIMER_INTERNAL_1=61,20 PRIMER_LEFT_1_TM=59.799 PRIMER_RIGHT_1_TM=59.894 PRIMER_INTERNAL_1_TM=59.505 PRIMER_LEFT_1_GC_PERCENT=52.381 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=11.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=95 PRIMER_PAIR_2_PENALTY=1.325097 PRIMER_LEFT_2_PENALTY=1.219185 PRIMER_RIGHT_2_PENALTY=0.105912 PRIMER_INTERNAL_2_PENALTY=0.495347 PRIMER_LEFT_2_SEQUENCE=TGGACATGCAGACAGTGTGTT PRIMER_RIGHT_2_SEQUENCE=CAAGATTTTATCCCAGGGCA PRIMER_INTERNAL_2_SEQUENCE=TCCTTTTCATCATTGCCCTT PRIMER_LEFT_2=37,21 PRIMER_RIGHT_2=130,20 PRIMER_INTERNAL_2=61,20 PRIMER_LEFT_2_TM=60.219 PRIMER_RIGHT_2_TM=59.894 PRIMER_INTERNAL_2_TM=59.505 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=11.2000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=94 PRIMER_PAIR_3_PENALTY=1.363838 PRIMER_LEFT_3_PENALTY=1.257926 PRIMER_RIGHT_3_PENALTY=0.105912 PRIMER_INTERNAL_3_PENALTY=3.236418 PRIMER_LEFT_3_SEQUENCE=TGCAGACAGTGTGTTCATTCC PRIMER_RIGHT_3_SEQUENCE=CAAGATTTTATCCCAGGGCA PRIMER_INTERNAL_3_SEQUENCE=CATCATTGCCCTTTTAAGTCTG PRIMER_LEFT_3=43,21 PRIMER_RIGHT_3=130,20 PRIMER_INTERNAL_3=68,22 PRIMER_LEFT_3_TM=59.742 PRIMER_RIGHT_3_TM=59.894 PRIMER_INTERNAL_3_TM=58.764 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=11.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=88 PRIMER_PAIR_4_PENALTY=1.458357 PRIMER_LEFT_4_PENALTY=1.352445 PRIMER_RIGHT_4_PENALTY=0.105912 PRIMER_INTERNAL_4_PENALTY=0.495347 PRIMER_LEFT_4_SEQUENCE=TGGACATGCAGACAGTGTGT PRIMER_RIGHT_4_SEQUENCE=CAAGATTTTATCCCAGGGCA PRIMER_INTERNAL_4_SEQUENCE=TCCTTTTCATCATTGCCCTT PRIMER_LEFT_4=37,20 PRIMER_RIGHT_4=130,20 PRIMER_INTERNAL_4=61,20 PRIMER_LEFT_4_TM=58.648 PRIMER_RIGHT_4_TM=59.894 PRIMER_INTERNAL_4_TM=59.505 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=11.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=94 = SEQUENCE_ID=MH1316 SEQUENCE_TEMPLATE=NTTNCATGCCTCCAGGTCGACTCTAGAGGATCCCCCTACGCCAACCAGGTCTCTCACCCAAAGCAAGGGGAAAAGGGCTGGATCCTGGGTGGCAAGCCAGTCCCCAAGGAAAGAATGGAGCGATGCAAATNAGAAAAAGGAACTCAAGCAGAAAGGCTCTTTTGCGGCTTCTAGTGGCAAGCCGNCCTGCATTTGCTTGTTCTGTCCCTTAGAGATGCCTCAGTTTCCTCAGCCATAAAATGGGCTGTTGTGAGGATTAAATATGANGTATAAAAATCTGTTGGATCAAGTAAGCCAAAAACATAGGGGCATTTAAAAAATTT SEQUENCE_INCLUDED_REGION=35,288 P3_COMMENT=CL is ACCAGGTCTCTCACCCAAAG P3_COMMENT=CR is TAATCCTCACAACAGCCCAT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.430845 PRIMER_LEFT_0_PENALTY=0.132570 PRIMER_RIGHT_0_PENALTY=0.298276 PRIMER_INTERNAL_0_PENALTY=2.003309 PRIMER_LEFT_0_SEQUENCE=CTACGCCAACCAGGTCTCTC PRIMER_RIGHT_0_SEQUENCE=ATTCTTTCCTTGGGGACTGG PRIMER_INTERNAL_0_SEQUENCE=AAAAGGGCTGGATCCTGG PRIMER_LEFT_0=35,20 PRIMER_RIGHT_0=115,20 PRIMER_INTERNAL_0=70,18 PRIMER_LEFT_0_TM=59.867 PRIMER_RIGHT_0_TM=60.298 PRIMER_INTERNAL_0_TM=60.003 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.556 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=0.430845 PRIMER_LEFT_1_PENALTY=0.132570 PRIMER_RIGHT_1_PENALTY=0.298276 PRIMER_INTERNAL_1_PENALTY=2.003309 PRIMER_LEFT_1_SEQUENCE=CTACGCCAACCAGGTCTCTC PRIMER_RIGHT_1_SEQUENCE=CCATTCTTTCCTTGGGGACT PRIMER_INTERNAL_1_SEQUENCE=AAAAGGGCTGGATCCTGG PRIMER_LEFT_1=35,20 PRIMER_RIGHT_1=117,20 PRIMER_INTERNAL_1=70,18 PRIMER_LEFT_1_TM=59.867 PRIMER_RIGHT_1_TM=60.298 PRIMER_INTERNAL_1_TM=60.003 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.556 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=0.458945 PRIMER_LEFT_2_PENALTY=0.132570 PRIMER_RIGHT_2_PENALTY=0.326376 PRIMER_INTERNAL_2_PENALTY=2.003309 PRIMER_LEFT_2_SEQUENCE=CTACGCCAACCAGGTCTCTC PRIMER_RIGHT_2_SEQUENCE=ATTTGCATCGCTCCATTCTT PRIMER_INTERNAL_2_SEQUENCE=AAAAGGGCTGGATCCTGG PRIMER_LEFT_2=35,20 PRIMER_RIGHT_2=129,20 PRIMER_INTERNAL_2=70,18 PRIMER_LEFT_2_TM=59.867 PRIMER_RIGHT_2_TM=59.674 PRIMER_INTERNAL_2_TM=60.003 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=55.556 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=95 PRIMER_PAIR_3_PENALTY=0.479375 PRIMER_LEFT_3_PENALTY=0.326376 PRIMER_RIGHT_3_PENALTY=0.152999 PRIMER_INTERNAL_3_PENALTY=0.513718 PRIMER_LEFT_3_SEQUENCE=AAGAATGGAGCGATGCAAAT PRIMER_RIGHT_3_SEQUENCE=GGACAGAACAAGCAAATGCA PRIMER_INTERNAL_3_SEQUENCE=TCTTTTGCGGCTTCTAGTGG PRIMER_LEFT_3=110,20 PRIMER_RIGHT_3=206,20 PRIMER_INTERNAL_3=157,20 PRIMER_LEFT_3_TM=59.674 PRIMER_RIGHT_3_TM=59.847 PRIMER_INTERNAL_3_TM=60.514 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=8.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=0.482436 PRIMER_LEFT_4_PENALTY=0.156061 PRIMER_RIGHT_4_PENALTY=0.326376 PRIMER_INTERNAL_4_PENALTY=2.003309 PRIMER_LEFT_4_SEQUENCE=GTCTCTCACCCAAAGCAAGG PRIMER_RIGHT_4_SEQUENCE=ATTTGCATCGCTCCATTCTT PRIMER_INTERNAL_4_SEQUENCE=AAAAGGGCTGGATCCTGG PRIMER_LEFT_4=48,20 PRIMER_RIGHT_4=129,20 PRIMER_INTERNAL_4=70,18 PRIMER_LEFT_4_TM=59.844 PRIMER_RIGHT_4_TM=59.674 PRIMER_INTERNAL_4_TM=60.003 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=55.556 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH1317 SEQUENCE_TEMPLATE=CCTCTCTCTCTTTCACACTCTGAGCCTGGCTTTTCCCAGGAATCCAATAGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGG SEQUENCE_INCLUDED_REGION=0,49 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH132 SEQUENCE_TEMPLATE=TTTCATGCTGCAGGTCGACTCTAGAGGATCCCTGACCCCTAGCCCTAGGAGCAGTGGGAAAAAAAAATACATTTTGACTGGAAGTAAACTGAGGAAACATGACATGGAGTCAGGGAGGGATTGTAGATCTTTCCCAGAGGTGTGATTTTTTAACAGACTGGCTTTTGGCGTTTGAGGAAAATCTCAACAACTCTCTGGACAGAATTTTCATAACTGTCCACTTGGCAGGGCTGATGTGTGTCTTGAAATAATTTTCAAGATTTGATTTTGAAATCTGAAATTTGATTTTATTGTATGTGCTC SEQUENCE_INCLUDED_REGION=31,271 P3_COMMENT=CL is TAGGAGCAGTGGGAAAAAAA P3_COMMENT=CR is TATTTCAAGACACACATCAGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.132377 PRIMER_LEFT_0_PENALTY=0.079648 PRIMER_RIGHT_0_PENALTY=0.052729 PRIMER_INTERNAL_0_PENALTY=2.023556 PRIMER_LEFT_0_SEQUENCE=ATGACATGGAGTCAGGGAGG PRIMER_RIGHT_0_SEQUENCE=GATTTTCCTCAAACGCCAAA PRIMER_INTERNAL_0_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=182,20 PRIMER_INTERNAL_0=127,22 PRIMER_LEFT_0_TM=59.920 PRIMER_RIGHT_0_TM=60.053 PRIMER_INTERNAL_0_TM=59.976 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=85 PRIMER_PAIR_1_PENALTY=0.271328 PRIMER_LEFT_1_PENALTY=0.079648 PRIMER_RIGHT_1_PENALTY=0.191680 PRIMER_INTERNAL_1_PENALTY=2.023556 PRIMER_LEFT_1_SEQUENCE=ATGACATGGAGTCAGGGAGG PRIMER_RIGHT_1_SEQUENCE=GAGATTTTCCTCAAACGCCA PRIMER_INTERNAL_1_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=184,20 PRIMER_INTERNAL_1=127,22 PRIMER_LEFT_1_TM=59.920 PRIMER_RIGHT_1_TM=60.192 PRIMER_INTERNAL_1_TM=59.976 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=11.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=87 PRIMER_PAIR_2_PENALTY=0.271328 PRIMER_LEFT_2_PENALTY=0.079648 PRIMER_RIGHT_2_PENALTY=0.191680 PRIMER_INTERNAL_2_PENALTY=2.023556 PRIMER_LEFT_2_SEQUENCE=ATGACATGGAGTCAGGGAGG PRIMER_RIGHT_2_SEQUENCE=TGAGATTTTCCTCAAACGCC PRIMER_INTERNAL_2_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT PRIMER_LEFT_2=98,20 PRIMER_RIGHT_2=185,20 PRIMER_INTERNAL_2=127,22 PRIMER_LEFT_2_TM=59.920 PRIMER_RIGHT_2_TM=60.192 PRIMER_INTERNAL_2_TM=59.976 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=11.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=88 PRIMER_PAIR_3_PENALTY=0.294872 PRIMER_LEFT_3_PENALTY=0.079648 PRIMER_RIGHT_3_PENALTY=0.215225 PRIMER_INTERNAL_3_PENALTY=2.023556 PRIMER_LEFT_3_SEQUENCE=ATGACATGGAGTCAGGGAGG PRIMER_RIGHT_3_SEQUENCE=TTTTCCTCAAACGCCAAAAG PRIMER_INTERNAL_3_SEQUENCE=TCTTTCCCAGAGGTGTGATTTT PRIMER_LEFT_3=98,20 PRIMER_RIGHT_3=180,20 PRIMER_INTERNAL_3=127,22 PRIMER_LEFT_3_TM=59.920 PRIMER_RIGHT_3_TM=60.215 PRIMER_INTERNAL_3_TM=59.976 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=0.297432 PRIMER_LEFT_4_PENALTY=0.217785 PRIMER_RIGHT_4_PENALTY=0.079648 PRIMER_INTERNAL_4_PENALTY=4.656911 PRIMER_LEFT_4_SEQUENCE=CTGACCCCTAGCCCTAGGAG PRIMER_RIGHT_4_SEQUENCE=CCTCCCTGACTCCATGTCAT PRIMER_INTERNAL_4_SEQUENCE=TTGACTGGAAGTAAACTGAGGAAA PRIMER_LEFT_4=31,20 PRIMER_RIGHT_4=117,20 PRIMER_INTERNAL_4=73,24 PRIMER_LEFT_4_TM=60.218 PRIMER_RIGHT_4_TM=59.920 PRIMER_INTERNAL_4_TM=59.343 PRIMER_LEFT_4_GC_PERCENT=65.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=37.500 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=8.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=6.3000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH1322 SEQUENCE_TEMPLATE=TGAAAATGTTGTTGAATTTCTAGTCTTTTGAGTNAGGAAGGTAAACTAGAAGGGNGTAGAGTNGAGGGCTAGGGAGCCTGCCCACCATGGGCACCAAACTNTTATTGAAAGAAGGATGAAATCAGTATATTTGTGGCAGGCTGAAAGAGTCAGTGGGCTCAGGCCTCATGTATCAGCAGCAGGTGAAGAACTAGGAGCACCACTGGAAAAGAGTCATCTCCTACAACTGAATCTGGGCTCTCACTCTTTTTTTGCCCTTAACTATGCTGCAGCCCACAAAGGATCTGTGGAATGGGGGTATCTTGTTAGTCAACTTGTAT SEQUENCE_INCLUDED_REGION=0,320 P3_COMMENT=CL is ATGTTGTTGAATTTCTAGTCTTTTG P3_COMMENT=CR is ACAAGATACCCCCATTCCAC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.166033 PRIMER_LEFT_0_PENALTY=0.156061 PRIMER_RIGHT_0_PENALTY=0.009973 PRIMER_INTERNAL_0_PENALTY=1.043279 PRIMER_LEFT_0_SEQUENCE=GGAGCACCACTGGAAAAGAG PRIMER_RIGHT_0_SEQUENCE=GCTGCAGCATAGTTAAGGGC PRIMER_INTERNAL_0_SEQUENCE=CAACTGAATCTGGGCTCTCA PRIMER_LEFT_0=193,20 PRIMER_RIGHT_0=272,20 PRIMER_INTERNAL_0=223,20 PRIMER_LEFT_0_TM=59.844 PRIMER_RIGHT_0_TM=60.010 PRIMER_INTERNAL_0_TM=58.957 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=10.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=0.166033 PRIMER_LEFT_1_PENALTY=0.156061 PRIMER_RIGHT_1_PENALTY=0.009973 PRIMER_INTERNAL_1_PENALTY=1.043279 PRIMER_LEFT_1_SEQUENCE=AGGAGCACCACTGGAAAAGA PRIMER_RIGHT_1_SEQUENCE=GCTGCAGCATAGTTAAGGGC PRIMER_INTERNAL_1_SEQUENCE=CAACTGAATCTGGGCTCTCA PRIMER_LEFT_1=192,20 PRIMER_RIGHT_1=272,20 PRIMER_INTERNAL_1=223,20 PRIMER_LEFT_1_TM=59.844 PRIMER_RIGHT_1_TM=60.010 PRIMER_INTERNAL_1_TM=58.957 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=10.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=81 PRIMER_PAIR_2_PENALTY=0.288917 PRIMER_LEFT_2_PENALTY=0.156061 PRIMER_RIGHT_2_PENALTY=0.132857 PRIMER_INTERNAL_2_PENALTY=1.043279 PRIMER_LEFT_2_SEQUENCE=GGAGCACCACTGGAAAAGAG PRIMER_RIGHT_2_SEQUENCE=GGCTGCAGCATAGTTAAGGG PRIMER_INTERNAL_2_SEQUENCE=CAACTGAATCTGGGCTCTCA PRIMER_LEFT_2=193,20 PRIMER_RIGHT_2=273,20 PRIMER_INTERNAL_2=223,20 PRIMER_LEFT_2_TM=59.844 PRIMER_RIGHT_2_TM=59.867 PRIMER_INTERNAL_2_TM=58.957 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=0.288917 PRIMER_LEFT_3_PENALTY=0.156061 PRIMER_RIGHT_3_PENALTY=0.132857 PRIMER_INTERNAL_3_PENALTY=1.043279 PRIMER_LEFT_3_SEQUENCE=GGAGCACCACTGGAAAAGAG PRIMER_RIGHT_3_SEQUENCE=GGGCTGCAGCATAGTTAAGG PRIMER_INTERNAL_3_SEQUENCE=CAACTGAATCTGGGCTCTCA PRIMER_LEFT_3=193,20 PRIMER_RIGHT_3=274,20 PRIMER_INTERNAL_3=223,20 PRIMER_LEFT_3_TM=59.844 PRIMER_RIGHT_3_TM=59.867 PRIMER_INTERNAL_3_TM=58.957 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.5000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=82 PRIMER_PAIR_4_PENALTY=0.288917 PRIMER_LEFT_4_PENALTY=0.156061 PRIMER_RIGHT_4_PENALTY=0.132857 PRIMER_INTERNAL_4_PENALTY=1.043279 PRIMER_LEFT_4_SEQUENCE=AGGAGCACCACTGGAAAAGA PRIMER_RIGHT_4_SEQUENCE=GGCTGCAGCATAGTTAAGGG PRIMER_INTERNAL_4_SEQUENCE=CAACTGAATCTGGGCTCTCA PRIMER_LEFT_4=192,20 PRIMER_RIGHT_4=273,20 PRIMER_INTERNAL_4=223,20 PRIMER_LEFT_4_TM=59.844 PRIMER_RIGHT_4_TM=59.867 PRIMER_INTERNAL_4_TM=58.957 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH1327 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAGAAATCTGTTCTCACCCCCACTCCTCCCCGCCTCATGGTCACCCTCCACTTTCTTTGGTCTGAAACCAATTGTGTAAATTAATTCACTTCTACATTGCTAATTGCTACGGAAGCTGAGAACTCAGAGGCTGCAAATACATCTTTTTGGGTTTTTTTGCCATTTTGAAAGTACAG SEQUENCE_INCLUDED_REGION=35,178 P3_COMMENT=CL is AGAAATCTGTTCTCACCCCC P3_COMMENT=CR is TTTGCAGCCTCTGAGTTCTC P3_COMMENT=CM is CGCCTCATGGTCACCCTC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.225404 PRIMER_LEFT_0_PENALTY=0.080729 PRIMER_RIGHT_0_PENALTY=0.144675 PRIMER_INTERNAL_0_PENALTY=1.786628 PRIMER_LEFT_0_SEQUENCE=TCACCCTCCACTTTCTTTGG PRIMER_RIGHT_0_SEQUENCE=TTGCAGCCTCTGAGTTCTCA PRIMER_INTERNAL_0_SEQUENCE=TTGCTAATTGCTACGGAAGC PRIMER_LEFT_0=77,20 PRIMER_RIGHT_0=172,20 PRIMER_INTERNAL_0=133,20 PRIMER_LEFT_0_TM=60.081 PRIMER_RIGHT_0_TM=59.855 PRIMER_INTERNAL_0_TM=58.213 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=96 PRIMER_PAIR_1_PENALTY=0.520875 PRIMER_LEFT_1_PENALTY=0.080729 PRIMER_RIGHT_1_PENALTY=0.440146 PRIMER_INTERNAL_1_PENALTY=3.501838 PRIMER_LEFT_1_SEQUENCE=TCACCCTCCACTTTCTTTGG PRIMER_RIGHT_1_SEQUENCE=GCAGCCTCTGAGTTCTCAGC PRIMER_INTERNAL_1_SEQUENCE=ACATTGCTAATTGCTACGGAA PRIMER_LEFT_1=77,20 PRIMER_RIGHT_1=170,20 PRIMER_INTERNAL_1=130,21 PRIMER_LEFT_1_TM=60.081 PRIMER_RIGHT_1_TM=60.440 PRIMER_INTERNAL_1_TM=57.498 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=94 PRIMER_PAIR_2_PENALTY=0.595518 PRIMER_LEFT_2_PENALTY=0.450843 PRIMER_RIGHT_2_PENALTY=0.144675 PRIMER_INTERNAL_2_PENALTY=1.786628 PRIMER_LEFT_2_SEQUENCE=TGGTCACCCTCCACTTTCTT PRIMER_RIGHT_2_SEQUENCE=TTGCAGCCTCTGAGTTCTCA PRIMER_INTERNAL_2_SEQUENCE=TTGCTAATTGCTACGGAAGC PRIMER_LEFT_2=74,20 PRIMER_RIGHT_2=172,20 PRIMER_INTERNAL_2=133,20 PRIMER_LEFT_2_TM=59.549 PRIMER_RIGHT_2_TM=59.855 PRIMER_INTERNAL_2_TM=58.213 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=0.639975 PRIMER_LEFT_3_PENALTY=0.080729 PRIMER_RIGHT_3_PENALTY=0.559246 PRIMER_INTERNAL_3_PENALTY=3.357217 PRIMER_LEFT_3_SEQUENCE=TCACCCTCCACTTTCTTTGG PRIMER_RIGHT_3_SEQUENCE=TGCAGCCTCTGAGTTCTCAG PRIMER_INTERNAL_3_SEQUENCE=CATTGCTAATTGCTACGGAAG PRIMER_LEFT_3=77,20 PRIMER_RIGHT_3=171,20 PRIMER_INTERNAL_3=131,21 PRIMER_LEFT_3_TM=60.081 PRIMER_RIGHT_3_TM=59.441 PRIMER_INTERNAL_3_TM=57.643 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=8.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.8000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=95 PRIMER_PAIR_4_PENALTY=0.803135 PRIMER_LEFT_4_PENALTY=0.362989 PRIMER_RIGHT_4_PENALTY=0.440146 PRIMER_INTERNAL_4_PENALTY=2.486265 PRIMER_LEFT_4_SEQUENCE=CATGGTCACCCTCCACTTTC PRIMER_RIGHT_4_SEQUENCE=GCAGCCTCTGAGTTCTCAGC PRIMER_INTERNAL_4_SEQUENCE=TTTGGTCTGAAACCAATTGTG PRIMER_LEFT_4=72,20 PRIMER_RIGHT_4=170,20 PRIMER_INTERNAL_4=92,21 PRIMER_LEFT_4_TM=60.363 PRIMER_RIGHT_4_TM=60.440 PRIMER_INTERNAL_4_TM=58.514 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_INTERNAL_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=9.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = SEQUENCE_ID=MH1328 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTATGTTCACTATGGTTCTTCTACTCTGTCTTCTGTGTACGCTCATGTAGGGAAATGTTAGAATCAACATGTGACCTTTGAGATTGGTTAAGAAAGGTTGAGTCAGGGAGACATTTAG SEQUENCE_INCLUDED_REGION=35,119 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.010555 PRIMER_LEFT_0_PENALTY=1.790576 PRIMER_RIGHT_0_PENALTY=2.219979 PRIMER_INTERNAL_0_PENALTY=3.022788 PRIMER_LEFT_0_SEQUENCE=TGTGTACGCTCATGTAGGGAA PRIMER_RIGHT_0_SEQUENCE=TGTCTCCCTGACTCAACCTTT PRIMER_INTERNAL_0_SEQUENCE=TCAACATGTGACCTTTGAGATTG PRIMER_LEFT_0=69,21 PRIMER_RIGHT_0=148,21 PRIMER_INTERNAL_0=99,23 PRIMER_LEFT_0_TM=59.209 PRIMER_RIGHT_0_TM=58.780 PRIMER_INTERNAL_0_TM=60.023 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_INTERNAL_0_GC_PERCENT=39.130 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=4.237825 PRIMER_LEFT_1_PENALTY=2.017846 PRIMER_RIGHT_1_PENALTY=2.219979 PRIMER_INTERNAL_1_PENALTY=3.022788 PRIMER_LEFT_1_SEQUENCE=TGTACGCTCATGTAGGGAAATG PRIMER_RIGHT_1_SEQUENCE=TGTCTCCCTGACTCAACCTTT PRIMER_INTERNAL_1_SEQUENCE=TCAACATGTGACCTTTGAGATTG PRIMER_LEFT_1=71,22 PRIMER_RIGHT_1=148,21 PRIMER_INTERNAL_1=99,23 PRIMER_LEFT_1_TM=60.018 PRIMER_RIGHT_1_TM=58.780 PRIMER_INTERNAL_1_TM=60.023 PRIMER_LEFT_1_GC_PERCENT=45.455 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=4.406569 PRIMER_LEFT_2_PENALTY=2.186590 PRIMER_RIGHT_2_PENALTY=2.219979 PRIMER_INTERNAL_2_PENALTY=3.022788 PRIMER_LEFT_2_SEQUENCE=CTGTGTACGCTCATGTAGGGA PRIMER_RIGHT_2_SEQUENCE=TGTCTCCCTGACTCAACCTTT PRIMER_INTERNAL_2_SEQUENCE=TCAACATGTGACCTTTGAGATTG PRIMER_LEFT_2=68,21 PRIMER_RIGHT_2=148,21 PRIMER_INTERNAL_2=99,23 PRIMER_LEFT_2_TM=58.813 PRIMER_RIGHT_2_TM=58.780 PRIMER_INTERNAL_2_TM=60.023 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=4.406569 PRIMER_LEFT_3_PENALTY=2.186590 PRIMER_RIGHT_3_PENALTY=2.219979 PRIMER_INTERNAL_3_PENALTY=3.022788 PRIMER_LEFT_3_SEQUENCE=TCTGTGTACGCTCATGTAGGG PRIMER_RIGHT_3_SEQUENCE=TGTCTCCCTGACTCAACCTTT PRIMER_INTERNAL_3_SEQUENCE=TCAACATGTGACCTTTGAGATTG PRIMER_LEFT_3=67,21 PRIMER_RIGHT_3=148,21 PRIMER_INTERNAL_3=99,23 PRIMER_LEFT_3_TM=58.813 PRIMER_RIGHT_3_TM=58.780 PRIMER_INTERNAL_3_TM=60.023 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.7000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=82 PRIMER_PAIR_4_PENALTY=4.409575 PRIMER_LEFT_4_PENALTY=2.189596 PRIMER_RIGHT_4_PENALTY=2.219979 PRIMER_INTERNAL_4_PENALTY=3.022788 PRIMER_LEFT_4_SEQUENCE=CTGTGTACGCTCATGTAGGGAA PRIMER_RIGHT_4_SEQUENCE=TGTCTCCCTGACTCAACCTTT PRIMER_INTERNAL_4_SEQUENCE=TCAACATGTGACCTTTGAGATTG PRIMER_LEFT_4=68,22 PRIMER_RIGHT_4=148,21 PRIMER_INTERNAL_4=99,23 PRIMER_LEFT_4_TM=60.190 PRIMER_RIGHT_4_TM=58.780 PRIMER_INTERNAL_4_TM=60.023 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_INTERNAL_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=81 = SEQUENCE_ID=MH1329 SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTTTGCTCCTGTCAGTCAGAGTNGCAGGTGCTTTTCTTTGCGTTCATGGAAATNATCCAATGGTATACACACAAGCGTCATCACTACAATTTTGGAAAAACCTGTGACTTGGAGGATTCGGGGATGTNTCATTGACTAGATGATTTGGGAGGCTCATCTGCACCCATTGGGCCTGTGCAGGTCTTGCAATAATCCTGTTGTCACCATCCAAGGACGTGAGGGCACCCTGCAAGG SEQUENCE_INCLUDED_REGION=33,241 P3_COMMENT=CL is TTTTGCTCCTGTCAGTCAGA P3_COMMENT=CR is CAACAGGATTATTGCAAGACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.654688 PRIMER_LEFT_0_PENALTY=0.008354 PRIMER_RIGHT_0_PENALTY=0.646334 PRIMER_INTERNAL_0_PENALTY=2.003166 PRIMER_LEFT_0_SEQUENCE=TGCTTTTCTTTGCGTTCATG PRIMER_RIGHT_0_SEQUENCE=CGAATCCTCCAAGTCACAGG PRIMER_INTERNAL_0_SEQUENCE=GCGTCATCACTACAATTTTGGA PRIMER_LEFT_0=68,20 PRIMER_RIGHT_0=159,20 PRIMER_INTERNAL_0=114,22 PRIMER_LEFT_0_TM=59.992 PRIMER_RIGHT_0_TM=60.646 PRIMER_INTERNAL_0_TM=60.003 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=92 PRIMER_PAIR_1_PENALTY=0.654688 PRIMER_LEFT_1_PENALTY=0.008354 PRIMER_RIGHT_1_PENALTY=0.646334 PRIMER_INTERNAL_1_PENALTY=2.003166 PRIMER_LEFT_1_SEQUENCE=TGCTTTTCTTTGCGTTCATG PRIMER_RIGHT_1_SEQUENCE=CCGAATCCTCCAAGTCACAG PRIMER_INTERNAL_1_SEQUENCE=GCGTCATCACTACAATTTTGGA PRIMER_LEFT_1=68,20 PRIMER_RIGHT_1=160,20 PRIMER_INTERNAL_1=114,22 PRIMER_LEFT_1_TM=59.992 PRIMER_RIGHT_1_TM=60.646 PRIMER_INTERNAL_1_TM=60.003 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=93 PRIMER_PAIR_2_PENALTY=0.782234 PRIMER_LEFT_2_PENALTY=0.646334 PRIMER_RIGHT_2_PENALTY=0.135900 PRIMER_INTERNAL_2_PENALTY=0.555419 PRIMER_LEFT_2_SEQUENCE=CTGTGACTTGGAGGATTCGG PRIMER_RIGHT_2_SEQUENCE=TATTGCAAGACCTGCACAGG PRIMER_INTERNAL_2_SEQUENCE=GATGATTTGGGAGGCTCATC PRIMER_LEFT_2=141,20 PRIMER_RIGHT_2=230,20 PRIMER_INTERNAL_2=178,20 PRIMER_LEFT_2_TM=60.646 PRIMER_RIGHT_2_TM=59.864 PRIMER_INTERNAL_2_TM=59.445 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=10.2000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=0.782234 PRIMER_LEFT_3_PENALTY=0.646334 PRIMER_RIGHT_3_PENALTY=0.135900 PRIMER_INTERNAL_3_PENALTY=0.555419 PRIMER_LEFT_3_SEQUENCE=CCTGTGACTTGGAGGATTCG PRIMER_RIGHT_3_SEQUENCE=TATTGCAAGACCTGCACAGG PRIMER_INTERNAL_3_SEQUENCE=GATGATTTGGGAGGCTCATC PRIMER_LEFT_3=140,20 PRIMER_RIGHT_3=230,20 PRIMER_INTERNAL_3=178,20 PRIMER_LEFT_3_TM=60.646 PRIMER_RIGHT_3_TM=59.864 PRIMER_INTERNAL_3_TM=59.445 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.6000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=91 PRIMER_PAIR_4_PENALTY=1.014609 PRIMER_LEFT_4_PENALTY=0.008354 PRIMER_RIGHT_4_PENALTY=1.006255 PRIMER_INTERNAL_4_PENALTY=2.021762 PRIMER_LEFT_4_SEQUENCE=TGCTTTTCTTTGCGTTCATG PRIMER_RIGHT_4_SEQUENCE=CCAAGTCACAGGTTTTTCCAA PRIMER_INTERNAL_4_SEQUENCE=CCAATGGTATACACACAAGCG PRIMER_LEFT_4=68,20 PRIMER_RIGHT_4=151,21 PRIMER_INTERNAL_4=96,21 PRIMER_LEFT_4_TM=59.992 PRIMER_RIGHT_4_TM=59.994 PRIMER_INTERNAL_4_TM=58.978 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=84 = SEQUENCE_ID=MH1337 SEQUENCE_TEMPLATE=TNNNTNCAGGTCGACTCTAGAGGATCCCCCTTCAGTGCTGAGGAAAGAAGTGGGAGCCGTATGGATACAGCAGGCAGAGAAGCACACATCATGTCCCAAAAGGCCAGGCATGACCAAGATCTGGCTGACGCTTCCAGAGAAAACGTGCAGACCCAGGATTTTNATGAGAAATCACTAGGTTTTTAAAAATGTTGACCAATCCCTCAAAGTAAATTTAAACCAATTGTGGATGAGTATTGTGAGGGCCAAATAAAGCCTGCCTGTGGGCCAGATGGCTGAATACCCAAAAGTCTGTGACCAGCCGGATAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTC SEQUENCE_INCLUDED_REGION=29,279 P3_COMMENT=CL is GAAAGAAGTGGGAGCCGTAT P3_COMMENT=CR is TTGGCCCTCACAATACTCAT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.368740 PRIMER_LEFT_0_PENALTY=0.156061 PRIMER_RIGHT_0_PENALTY=0.212679 PRIMER_INTERNAL_0_PENALTY=0.032945 PRIMER_LEFT_0_SEQUENCE=GTGCTGAGGAAAGAAGTGGG PRIMER_RIGHT_0_SEQUENCE=TCAGCCAGATCTTGGTCATG PRIMER_INTERNAL_0_SEQUENCE=AGCACACATCATGTCCCAAA PRIMER_LEFT_0=34,20 PRIMER_RIGHT_0=127,20 PRIMER_INTERNAL_0=80,20 PRIMER_LEFT_0_TM=59.844 PRIMER_RIGHT_0_TM=59.787 PRIMER_INTERNAL_0_TM=59.967 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=94 PRIMER_PAIR_1_PENALTY=0.549303 PRIMER_LEFT_1_PENALTY=0.117817 PRIMER_RIGHT_1_PENALTY=0.431486 PRIMER_INTERNAL_1_PENALTY=0.032945 PRIMER_LEFT_1_SEQUENCE=AGCCGTATGGATACAGCAGG PRIMER_RIGHT_1_SEQUENCE=CACGTTTTCTCTGGAAGCGT PRIMER_INTERNAL_1_SEQUENCE=AGCACACATCATGTCCCAAA PRIMER_LEFT_1=54,20 PRIMER_RIGHT_1=146,20 PRIMER_INTERNAL_1=80,20 PRIMER_LEFT_1_TM=60.118 PRIMER_RIGHT_1_TM=60.431 PRIMER_INTERNAL_1_TM=59.967 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=9.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=93 PRIMER_PAIR_2_PENALTY=0.551958 PRIMER_LEFT_2_PENALTY=0.117817 PRIMER_RIGHT_2_PENALTY=0.434141 PRIMER_INTERNAL_2_PENALTY=0.032945 PRIMER_LEFT_2_SEQUENCE=AGCCGTATGGATACAGCAGG PRIMER_RIGHT_2_SEQUENCE=TCTGCACGTTTTCTCTGGAA PRIMER_INTERNAL_2_SEQUENCE=AGCACACATCATGTCCCAAA PRIMER_LEFT_2=54,20 PRIMER_RIGHT_2=150,20 PRIMER_INTERNAL_2=80,20 PRIMER_LEFT_2_TM=60.118 PRIMER_RIGHT_2_TM=59.566 PRIMER_INTERNAL_2_TM=59.967 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=97 PRIMER_PAIR_3_PENALTY=0.640260 PRIMER_LEFT_3_PENALTY=0.117817 PRIMER_RIGHT_3_PENALTY=0.522443 PRIMER_INTERNAL_3_PENALTY=0.032945 PRIMER_LEFT_3_SEQUENCE=AGCCGTATGGATACAGCAGG PRIMER_RIGHT_3_SEQUENCE=ACGTTTTCTCTGGAAGCGTC PRIMER_INTERNAL_3_SEQUENCE=AGCACACATCATGTCCCAAA PRIMER_LEFT_3=54,20 PRIMER_RIGHT_3=145,20 PRIMER_INTERNAL_3=80,20 PRIMER_LEFT_3_TM=60.118 PRIMER_RIGHT_3_TM=59.478 PRIMER_INTERNAL_3_TM=59.967 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=9.6000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=92 PRIMER_PAIR_4_PENALTY=0.774911 PRIMER_LEFT_4_PENALTY=0.117817 PRIMER_RIGHT_4_PENALTY=0.657094 PRIMER_INTERNAL_4_PENALTY=0.032945 PRIMER_LEFT_4_SEQUENCE=AGCCGTATGGATACAGCAGG PRIMER_RIGHT_4_SEQUENCE=TTTTCTCTGGAAGCGTCAGC PRIMER_INTERNAL_4_SEQUENCE=AGCACACATCATGTCCCAAA PRIMER_LEFT_4=54,20 PRIMER_RIGHT_4=142,20 PRIMER_INTERNAL_4=80,20 PRIMER_LEFT_4_TM=60.118 PRIMER_RIGHT_4_TM=60.657 PRIMER_INTERNAL_4_TM=59.967 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH139 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAAAAGTNGGAAGGCAGGACTGTGTTTTGGAGGACAAAAAGTAAAATCTTTTTATATCTTTATTTTTTAATTTTATTTTTTTTCAGGCATATAGACATACATATCCATTATACAACAGAAAAGGGGGCTGGAAAAGAAAGGTCAAGTGAGATTTCAGATATTCTTAAATGCAAGGCTGACAAATTTGGGCTTGATTCTGTAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA SEQUENCE_INCLUDED_REGION=35,204 P3_COMMENT=CL is GAAGGCAGGACTGTGTTTTG P3_COMMENT=CR is AATCAAGCCCAAATTTGTCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.090051 PRIMER_LEFT_0_PENALTY=0.103024 PRIMER_RIGHT_0_PENALTY=0.987026 PRIMER_INTERNAL_0_PENALTY=5.175845 PRIMER_LEFT_0_SEQUENCE=TACAACAGAAAAGGGGGCTG PRIMER_RIGHT_0_SEQUENCE=AGCCCAAATTTGTCAGCCTT PRIMER_INTERNAL_0_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA PRIMER_LEFT_0=149,20 PRIMER_RIGHT_0=229,20 PRIMER_INTERNAL_0=172,25 PRIMER_LEFT_0_TM=60.103 PRIMER_RIGHT_0_TM=60.987 PRIMER_INTERNAL_0_TM=59.824 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=1.090051 PRIMER_LEFT_1_PENALTY=0.103024 PRIMER_RIGHT_1_PENALTY=0.987026 PRIMER_INTERNAL_1_PENALTY=5.175845 PRIMER_LEFT_1_SEQUENCE=TACAACAGAAAAGGGGGCTG PRIMER_RIGHT_1_SEQUENCE=AAGCCCAAATTTGTCAGCCT PRIMER_INTERNAL_1_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA PRIMER_LEFT_1=149,20 PRIMER_RIGHT_1=230,20 PRIMER_INTERNAL_1=172,25 PRIMER_LEFT_1_TM=60.103 PRIMER_RIGHT_1_TM=60.987 PRIMER_INTERNAL_1_TM=59.824 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=1.169167 PRIMER_LEFT_2_PENALTY=0.103024 PRIMER_RIGHT_2_PENALTY=1.066143 PRIMER_INTERNAL_2_PENALTY=5.175845 PRIMER_LEFT_2_SEQUENCE=TACAACAGAAAAGGGGGCTG PRIMER_RIGHT_2_SEQUENCE=GCCCAAATTTGTCAGCCTT PRIMER_INTERNAL_2_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA PRIMER_LEFT_2=149,20 PRIMER_RIGHT_2=228,19 PRIMER_INTERNAL_2=172,25 PRIMER_LEFT_2_TM=60.103 PRIMER_RIGHT_2_TM=60.066 PRIMER_INTERNAL_2_TM=59.824 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=47.368 PRIMER_INTERNAL_2_GC_PERCENT=36.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=1.169167 PRIMER_LEFT_3_PENALTY=0.103024 PRIMER_RIGHT_3_PENALTY=1.066143 PRIMER_INTERNAL_3_PENALTY=5.175845 PRIMER_LEFT_3_SEQUENCE=TACAACAGAAAAGGGGGCTG PRIMER_RIGHT_3_SEQUENCE=AAGCCCAAATTTGTCAGCC PRIMER_INTERNAL_3_SEQUENCE=AAGAAAGGTCAAGTGAGATTTCAGA PRIMER_LEFT_3=149,20 PRIMER_RIGHT_3=230,19 PRIMER_INTERNAL_3=172,25 PRIMER_LEFT_3_TM=60.103 PRIMER_RIGHT_3_TM=60.066 PRIMER_INTERNAL_3_TM=59.824 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=47.368 PRIMER_INTERNAL_3_GC_PERCENT=36.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=5.00 PRIMER_PAIR_3_PRODUCT_SIZE=82 PRIMER_PAIR_4_PENALTY=1.172651 PRIMER_LEFT_4_PENALTY=0.103024 PRIMER_RIGHT_4_PENALTY=1.069627 PRIMER_INTERNAL_4_PENALTY=5.011655 PRIMER_LEFT_4_SEQUENCE=TACAACAGAAAAGGGGGCTG PRIMER_RIGHT_4_SEQUENCE=GAATCAAGCCCAAATTTGTCA PRIMER_INTERNAL_4_SEQUENCE=TTTCAGATATTCTTAAATGCAAGGC PRIMER_LEFT_4=149,20 PRIMER_RIGHT_4=235,21 PRIMER_INTERNAL_4=190,25 PRIMER_LEFT_4_TM=60.103 PRIMER_RIGHT_4_TM=59.930 PRIMER_INTERNAL_4_TM=60.012 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_INTERNAL_4_GC_PERCENT=32.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH141 SEQUENCE_TEMPLATE=CCTGCAGGTCGACTCTAGAGGATCCCCCTGAATGCAAACATCACTGAGAAGTTTCTGAGAATGCTTCTGTTTGATTTTATATGAAGAAATTCCCGTTTCCAACGAAATCTTCAGAGGGGTACCGCGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAACTGCATTAATGAATCGGC SEQUENCE_INCLUDED_REGION=27,89 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.132239 PRIMER_LEFT_0_PENALTY=2.678193 PRIMER_RIGHT_0_PENALTY=0.454047 PRIMER_INTERNAL_0_PENALTY=3.855762 PRIMER_LEFT_0_SEQUENCE=TGAATGCAAACATCACTGAGAA PRIMER_RIGHT_0_SEQUENCE=GAAGATTTCGTTGGAAACGG PRIMER_INTERNAL_0_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA PRIMER_LEFT_0=28,22 PRIMER_RIGHT_0=111,20 PRIMER_INTERNAL_0=51,23 PRIMER_LEFT_0_TM=59.322 PRIMER_RIGHT_0_TM=59.546 PRIMER_INTERNAL_0_TM=59.144 PRIMER_LEFT_0_GC_PERCENT=36.364 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=9.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=84 PRIMER_PAIR_1_PENALTY=3.220455 PRIMER_LEFT_1_PENALTY=2.678193 PRIMER_RIGHT_1_PENALTY=0.542262 PRIMER_INTERNAL_1_PENALTY=3.855762 PRIMER_LEFT_1_SEQUENCE=TGAATGCAAACATCACTGAGAA PRIMER_RIGHT_1_SEQUENCE=ATTTCGTTGGAAACGGGAAT PRIMER_INTERNAL_1_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA PRIMER_LEFT_1=28,22 PRIMER_RIGHT_1=107,20 PRIMER_INTERNAL_1=51,23 PRIMER_LEFT_1_TM=59.322 PRIMER_RIGHT_1_TM=60.542 PRIMER_INTERNAL_1_TM=59.144 PRIMER_LEFT_1_GC_PERCENT=36.364 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=34.783 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=3.528058 PRIMER_LEFT_2_PENALTY=3.074011 PRIMER_RIGHT_2_PENALTY=0.454047 PRIMER_INTERNAL_2_PENALTY=3.855762 PRIMER_LEFT_2_SEQUENCE=CTGAATGCAAACATCACTGAGA PRIMER_RIGHT_2_SEQUENCE=GAAGATTTCGTTGGAAACGG PRIMER_INTERNAL_2_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA PRIMER_LEFT_2=27,22 PRIMER_RIGHT_2=111,20 PRIMER_INTERNAL_2=51,23 PRIMER_LEFT_2_TM=58.926 PRIMER_RIGHT_2_TM=59.546 PRIMER_INTERNAL_2_TM=59.144 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=34.783 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=85 PRIMER_PAIR_3_PENALTY=3.616274 PRIMER_LEFT_3_PENALTY=3.074011 PRIMER_RIGHT_3_PENALTY=0.542262 PRIMER_INTERNAL_3_PENALTY=3.855762 PRIMER_LEFT_3_SEQUENCE=CTGAATGCAAACATCACTGAGA PRIMER_RIGHT_3_SEQUENCE=ATTTCGTTGGAAACGGGAAT PRIMER_INTERNAL_3_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA PRIMER_LEFT_3=27,22 PRIMER_RIGHT_3=107,20 PRIMER_INTERNAL_3=51,23 PRIMER_LEFT_3_TM=58.926 PRIMER_RIGHT_3_TM=60.542 PRIMER_INTERNAL_3_TM=59.144 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=34.783 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=81 PRIMER_PAIR_4_PENALTY=3.634131 PRIMER_LEFT_4_PENALTY=3.180084 PRIMER_RIGHT_4_PENALTY=0.454047 PRIMER_INTERNAL_4_PENALTY=3.855762 PRIMER_LEFT_4_SEQUENCE=TGAATGCAAACATCACTGAGA PRIMER_RIGHT_4_SEQUENCE=GAAGATTTCGTTGGAAACGG PRIMER_INTERNAL_4_SEQUENCE=TTTCTGAGAATGCTTCTGTTTGA PRIMER_LEFT_4=28,21 PRIMER_RIGHT_4=111,20 PRIMER_INTERNAL_4=51,23 PRIMER_LEFT_4_TM=57.820 PRIMER_RIGHT_4_TM=59.546 PRIMER_INTERNAL_4_TM=59.144 PRIMER_LEFT_4_GC_PERCENT=38.095 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=34.783 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=9.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=84 = SEQUENCE_ID=MH142 SEQUENCE_TEMPLATE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCTGTAACAGTCTGGGTTGATTTGTTTTCCGATTTTGNTTTTTTTTAAATCACAGAAACATTTAGTTTCTTCATCTATACAATGGTGATAAAAATCTGTTACATGATCCTGACTTCAGGGCCATAGATAATTTGTCCTGAAGGGGATCCCTGGCCCGCTCTGTCCTTCAATATAGGTAGTAACTGCGGAACGTGAAGATCTAGAGCATGCTTTCCTCATGAAAATGCA SEQUENCE_INCLUDED_REGION=34,227 P3_COMMENT=CL is TCTGGGTTGATTTGTTTTCC P3_COMMENT=CR is TTTCATGAGGAAAGCATGCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.335696 PRIMER_LEFT_0_PENALTY=0.209870 PRIMER_RIGHT_0_PENALTY=0.125826 PRIMER_INTERNAL_0_PENALTY=0.132549 PRIMER_LEFT_0_SEQUENCE=CCTGACTTCAGGGCCATAGA PRIMER_RIGHT_0_SEQUENCE=AGATCTTCACGTTCCGCAGT PRIMER_INTERNAL_0_SEQUENCE=ATTTGTCCTGAAGGGGATCC PRIMER_LEFT_0=140,20 PRIMER_RIGHT_0=233,20 PRIMER_INTERNAL_0=162,20 PRIMER_LEFT_0_TM=60.210 PRIMER_RIGHT_0_TM=59.874 PRIMER_INTERNAL_0_TM=60.133 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=5.6000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=94 PRIMER_PAIR_1_PENALTY=0.335696 PRIMER_LEFT_1_PENALTY=0.209870 PRIMER_RIGHT_1_PENALTY=0.125826 PRIMER_INTERNAL_1_PENALTY=0.132549 PRIMER_LEFT_1_SEQUENCE=TCCTGACTTCAGGGCCATAG PRIMER_RIGHT_1_SEQUENCE=AGATCTTCACGTTCCGCAGT PRIMER_INTERNAL_1_SEQUENCE=ATTTGTCCTGAAGGGGATCC PRIMER_LEFT_1=139,20 PRIMER_RIGHT_1=233,20 PRIMER_INTERNAL_1=162,20 PRIMER_LEFT_1_TM=60.210 PRIMER_RIGHT_1_TM=59.874 PRIMER_INTERNAL_1_TM=60.133 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=5.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=95 PRIMER_PAIR_2_PENALTY=0.472724 PRIMER_LEFT_2_PENALTY=0.209870 PRIMER_RIGHT_2_PENALTY=0.262854 PRIMER_INTERNAL_2_PENALTY=0.132549 PRIMER_LEFT_2_SEQUENCE=CCTGACTTCAGGGCCATAGA PRIMER_RIGHT_2_SEQUENCE=GATCTTCACGTTCCGCAGTT PRIMER_INTERNAL_2_SEQUENCE=ATTTGTCCTGAAGGGGATCC PRIMER_LEFT_2=140,20 PRIMER_RIGHT_2=232,20 PRIMER_INTERNAL_2=162,20 PRIMER_LEFT_2_TM=60.210 PRIMER_RIGHT_2_TM=60.263 PRIMER_INTERNAL_2_TM=60.133 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=5.6000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=93 PRIMER_PAIR_3_PENALTY=0.472724 PRIMER_LEFT_3_PENALTY=0.209870 PRIMER_RIGHT_3_PENALTY=0.262854 PRIMER_INTERNAL_3_PENALTY=0.132549 PRIMER_LEFT_3_SEQUENCE=TCCTGACTTCAGGGCCATAG PRIMER_RIGHT_3_SEQUENCE=GATCTTCACGTTCCGCAGTT PRIMER_INTERNAL_3_SEQUENCE=ATTTGTCCTGAAGGGGATCC PRIMER_LEFT_3=139,20 PRIMER_RIGHT_3=232,20 PRIMER_INTERNAL_3=162,20 PRIMER_LEFT_3_TM=60.210 PRIMER_RIGHT_3_TM=60.263 PRIMER_INTERNAL_3_TM=60.133 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=5.9000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=0.618308 PRIMER_LEFT_4_PENALTY=0.492482 PRIMER_RIGHT_4_PENALTY=0.125826 PRIMER_INTERNAL_4_PENALTY=0.132549 PRIMER_LEFT_4_SEQUENCE=ATCCTGACTTCAGGGCCATA PRIMER_RIGHT_4_SEQUENCE=AGATCTTCACGTTCCGCAGT PRIMER_INTERNAL_4_SEQUENCE=ATTTGTCCTGAAGGGGATCC PRIMER_LEFT_4=138,20 PRIMER_RIGHT_4=233,20 PRIMER_INTERNAL_4=162,20 PRIMER_LEFT_4_TM=59.508 PRIMER_RIGHT_4_TM=59.874 PRIMER_INTERNAL_4_TM=60.133 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=7.4000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=96 = SEQUENCE_ID=MH150 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTTTGATGCTTGTTTGTAACTGTCCAAAGGAAACCACTGCAACCTTCAAGCATGTCTGTGTTACTCTATTTTGTTCCAGCCACCTGTGGCATTTCCAAAATATGATAATCTCTGCCACCATACTGCTTTAAACACAAATAGAATCTGGCAGCAAAATATAGCATAAGCTTACTTCTAAATCAAAGGCTACCATCAGTACCTTAGCACATTTAAAAAATAAAAACCAACACTGCCCACTTCAATGAATGCTTTCAGCACACACCACAAAGGCTACTGGGG SEQUENCE_INCLUDED_REGION=35,283 P3_COMMENT=CL is AAACCACTGCAACCTTCAAG P3_COMMENT=CR is TGGGCAGTGTTGGTTTTTAT P3_COMMENT=CM is GTGTTACTCTATTTTGTTCCAGCC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.082828 PRIMER_LEFT_0_PENALTY=0.536418 PRIMER_RIGHT_0_PENALTY=0.546410 PRIMER_INTERNAL_0_PENALTY=0.298898 PRIMER_LEFT_0_SEQUENCE=CCTGCTTTGATGCTTGTTTG PRIMER_RIGHT_0_SEQUENCE=ACAGGTGGCTGGAACAAAAT PRIMER_INTERNAL_0_SEQUENCE=AACCACTGCAACCTTCAAGC PRIMER_LEFT_0=35,20 PRIMER_RIGHT_0=125,20 PRIMER_INTERNAL_0=70,20 PRIMER_LEFT_0_TM=59.464 PRIMER_RIGHT_0_TM=59.454 PRIMER_INTERNAL_0_TM=60.299 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=91 PRIMER_PAIR_1_PENALTY=1.236370 PRIMER_LEFT_1_PENALTY=0.528861 PRIMER_RIGHT_1_PENALTY=0.707509 PRIMER_INTERNAL_1_PENALTY=5.467656 PRIMER_LEFT_1_SEQUENCE=CAAAGGAAACCACTGCAACC PRIMER_RIGHT_1_SEQUENCE=ATTTTGGAAATGCCACAGGT PRIMER_INTERNAL_1_SEQUENCE=TGTTACTCTATTTTGTTCCAGCC PRIMER_LEFT_1=63,20 PRIMER_RIGHT_1=139,20 PRIMER_INTERNAL_1=97,23 PRIMER_LEFT_1_TM=60.529 PRIMER_RIGHT_1_TM=59.292 PRIMER_INTERNAL_1_TM=57.532 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=1.236370 PRIMER_LEFT_2_PENALTY=0.528861 PRIMER_RIGHT_2_PENALTY=0.707509 PRIMER_INTERNAL_2_PENALTY=5.467656 PRIMER_LEFT_2_SEQUENCE=CCAAAGGAAACCACTGCAAC PRIMER_RIGHT_2_SEQUENCE=ATTTTGGAAATGCCACAGGT PRIMER_INTERNAL_2_SEQUENCE=TGTTACTCTATTTTGTTCCAGCC PRIMER_LEFT_2=62,20 PRIMER_RIGHT_2=139,20 PRIMER_INTERNAL_2=97,23 PRIMER_LEFT_2_TM=60.529 PRIMER_RIGHT_2_TM=59.292 PRIMER_INTERNAL_2_TM=57.532 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=78 PRIMER_PAIR_3_PENALTY=1.408997 PRIMER_LEFT_3_PENALTY=0.298898 PRIMER_RIGHT_3_PENALTY=1.110099 PRIMER_INTERNAL_3_PENALTY=0.546410 PRIMER_LEFT_3_SEQUENCE=AACCACTGCAACCTTCAAGC PRIMER_RIGHT_3_SEQUENCE=AAAGCAGTATGGTGGCAGAGA PRIMER_INTERNAL_3_SEQUENCE=ATTTTGTTCCAGCCACCTGT PRIMER_LEFT_3=70,20 PRIMER_RIGHT_3=167,21 PRIMER_INTERNAL_3=106,20 PRIMER_LEFT_3_TM=60.299 PRIMER_RIGHT_3_TM=59.890 PRIMER_INTERNAL_3_TM=59.454 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=1.559540 PRIMER_LEFT_4_PENALTY=0.449441 PRIMER_RIGHT_4_PENALTY=1.110099 PRIMER_INTERNAL_4_PENALTY=0.546410 PRIMER_LEFT_4_SEQUENCE=CACTGCAACCTTCAAGCATG PRIMER_RIGHT_4_SEQUENCE=AAAGCAGTATGGTGGCAGAGA PRIMER_INTERNAL_4_SEQUENCE=ATTTTGTTCCAGCCACCTGT PRIMER_LEFT_4=73,20 PRIMER_RIGHT_4=167,21 PRIMER_INTERNAL_4=106,20 PRIMER_LEFT_4_TM=60.449 PRIMER_RIGHT_4_TM=59.890 PRIMER_INTERNAL_4_TM=59.454 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=95 = SEQUENCE_ID=MH152 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTATAATTATAAGACACCTTTATGCCCCAGGACATCCAGGTTCTTTACAGACTTTAGCCCTCTCAGGAGGCTGGTGGCATTTTGCCATTTTACAGATGAAGGTGATTATAAACAAAGCATTGAAAAGATGTATTGCACAGCAGGTTGCAGGAATTTGTGCCAGTATAAAACACCTTCTTTGTTCGCTACTAGTGTTAACATCTATAATGTGCTTAGATTGTTTTTCTCATCCTTAATACAGGGTACCGAG SEQUENCE_INCLUDED_REGION=35,252 P3_COMMENT=CL is TAAGACACCTTTATGCCCCA P3_COMMENT=CR is AAGGATGAGAAAAACAATCTAAGC P3_COMMENT=CM is GACATCCAGGTTCTTTACAGACTT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.321121 PRIMER_LEFT_0_PENALTY=0.064105 PRIMER_RIGHT_0_PENALTY=0.257016 PRIMER_INTERNAL_0_PENALTY=2.627348 PRIMER_LEFT_0_SEQUENCE=TGGCATTTTGCCATTTTACA PRIMER_RIGHT_0_SEQUENCE=TACTGGCACAAATTCCTGCA PRIMER_INTERNAL_0_SEQUENCE=TGAAAAGATGTATTGCACAGCA PRIMER_LEFT_0=111,20 PRIMER_RIGHT_0=201,20 PRIMER_INTERNAL_0=157,22 PRIMER_LEFT_0_TM=59.936 PRIMER_RIGHT_0_TM=60.257 PRIMER_INTERNAL_0_TM=59.373 PRIMER_LEFT_0_GC_PERCENT=35.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=36.364 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.0000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=91 PRIMER_PAIR_1_PENALTY=0.323809 PRIMER_LEFT_1_PENALTY=0.064105 PRIMER_RIGHT_1_PENALTY=0.259704 PRIMER_INTERNAL_1_PENALTY=4.031619 PRIMER_LEFT_1_SEQUENCE=TGGCATTTTGCCATTTTACA PRIMER_RIGHT_1_SEQUENCE=AAATTCCTGCAACCTGCTGT PRIMER_INTERNAL_1_SEQUENCE=CAAAGCATTGAAAAGATGTATTGC PRIMER_LEFT_1=111,20 PRIMER_RIGHT_1=192,20 PRIMER_INTERNAL_1=149,24 PRIMER_LEFT_1_TM=59.936 PRIMER_RIGHT_1_TM=59.740 PRIMER_INTERNAL_1_TM=60.032 PRIMER_LEFT_1_GC_PERCENT=35.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=33.333 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.0000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=0.323809 PRIMER_LEFT_2_PENALTY=0.064105 PRIMER_RIGHT_2_PENALTY=0.259704 PRIMER_INTERNAL_2_PENALTY=4.031619 PRIMER_LEFT_2_SEQUENCE=TGGCATTTTGCCATTTTACA PRIMER_RIGHT_2_SEQUENCE=ACAAATTCCTGCAACCTGCT PRIMER_INTERNAL_2_SEQUENCE=AAAGCATTGAAAAGATGTATTGCA PRIMER_LEFT_2=111,20 PRIMER_RIGHT_2=194,20 PRIMER_INTERNAL_2=150,24 PRIMER_LEFT_2_TM=59.936 PRIMER_RIGHT_2_TM=59.740 PRIMER_INTERNAL_2_TM=60.032 PRIMER_LEFT_2_GC_PERCENT=35.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=29.167 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.0000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=84 PRIMER_PAIR_3_PENALTY=0.642473 PRIMER_LEFT_3_PENALTY=0.578368 PRIMER_RIGHT_3_PENALTY=0.064105 PRIMER_INTERNAL_3_PENALTY=0.475706 PRIMER_LEFT_3_SEQUENCE=ACCTTTATGCCCCAGGACAT PRIMER_RIGHT_3_SEQUENCE=TGTAAAATGGCAAAATGCCA PRIMER_INTERNAL_3_SEQUENCE=TTTAGCCCTCTCAGGAGGCT PRIMER_LEFT_3=52,20 PRIMER_RIGHT_3=130,20 PRIMER_INTERNAL_3=89,20 PRIMER_LEFT_3_TM=60.578 PRIMER_RIGHT_3_TM=59.936 PRIMER_INTERNAL_3_TM=60.476 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=35.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=7.00 PRIMER_INTERNAL_3_SELF_END=7.00 PRIMER_LEFT_3_END_STABILITY=6.3000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=0.937502 PRIMER_LEFT_4_PENALTY=0.680486 PRIMER_RIGHT_4_PENALTY=0.257016 PRIMER_INTERNAL_4_PENALTY=2.627348 PRIMER_LEFT_4_SEQUENCE=GGTGGCATTTTGCCATTTTA PRIMER_RIGHT_4_SEQUENCE=TACTGGCACAAATTCCTGCA PRIMER_INTERNAL_4_SEQUENCE=TGAAAAGATGTATTGCACAGCA PRIMER_LEFT_4=109,20 PRIMER_RIGHT_4=201,20 PRIMER_INTERNAL_4=157,22 PRIMER_LEFT_4_TM=60.680 PRIMER_RIGHT_4_TM=60.257 PRIMER_INTERNAL_4_TM=59.373 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=36.364 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=8.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=93 = SEQUENCE_ID=MH154 SEQUENCE_TEMPLATE=GTTTTGATGCTGCAGGTCGACTCTAGAGGATCCCCTAAGAAAACAGTGTNGTAATTCCCTGGCTTTGCACATGTTCCTTCATCTCATCATCTGCCCTAAAGTNGATGCTGCTCCAATATTCAATGTGGATTTATACAAACCACTGCATGACACAGCAAACATGGTTGGACCTCATTCATATACCCCTGGCTCACCATGCAAATCACCTGCAGACAGTTCTCACACATGGCCAAGGCTTTTCTGTGTCTGTGGGCAGGCTTGGGCCATGAAGTGTGCATGTGGCCAGATCAGAGAGTTAATTCCCCAGGGAGTGACCTTCTACTACCGTGAAGGGCAGGATGTGGTAGATAAGTAGTTCAGTTTCCTCA SEQUENCE_INCLUDED_REGION=34,334 P3_COMMENT=CL is GTAATTCCCTGGCTTTGCAC P3_COMMENT=CR is TTATCTACCACATCCTGCCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.170673 PRIMER_LEFT_0_PENALTY=0.008216 PRIMER_RIGHT_0_PENALTY=0.162457 PRIMER_INTERNAL_0_PENALTY=0.112124 PRIMER_LEFT_0_SEQUENCE=CACAGCAAACATGGTTGGAC PRIMER_RIGHT_0_SEQUENCE=TGGCCATGTGTGAGAACTGT PRIMER_INTERNAL_0_SEQUENCE=CTCACCATGCAAATCACCTG PRIMER_LEFT_0=150,20 PRIMER_RIGHT_0=231,20 PRIMER_INTERNAL_0=189,20 PRIMER_LEFT_0_TM=60.008 PRIMER_RIGHT_0_TM=60.162 PRIMER_INTERNAL_0_TM=60.112 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=0.170673 PRIMER_LEFT_1_PENALTY=0.008216 PRIMER_RIGHT_1_PENALTY=0.162457 PRIMER_INTERNAL_1_PENALTY=0.112124 PRIMER_LEFT_1_SEQUENCE=ACACAGCAAACATGGTTGGA PRIMER_RIGHT_1_SEQUENCE=TGGCCATGTGTGAGAACTGT PRIMER_INTERNAL_1_SEQUENCE=CTCACCATGCAAATCACCTG PRIMER_LEFT_1=149,20 PRIMER_RIGHT_1=231,20 PRIMER_INTERNAL_1=189,20 PRIMER_LEFT_1_TM=60.008 PRIMER_RIGHT_1_TM=60.162 PRIMER_INTERNAL_1_TM=60.112 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=0.170673 PRIMER_LEFT_2_PENALTY=0.008216 PRIMER_RIGHT_2_PENALTY=0.162457 PRIMER_INTERNAL_2_PENALTY=0.112124 PRIMER_LEFT_2_SEQUENCE=GACACAGCAAACATGGTTGG PRIMER_RIGHT_2_SEQUENCE=TGGCCATGTGTGAGAACTGT PRIMER_INTERNAL_2_SEQUENCE=CTCACCATGCAAATCACCTG PRIMER_LEFT_2=148,20 PRIMER_RIGHT_2=231,20 PRIMER_INTERNAL_2=189,20 PRIMER_LEFT_2_TM=60.008 PRIMER_RIGHT_2_TM=60.162 PRIMER_INTERNAL_2_TM=60.112 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.1000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=84 PRIMER_PAIR_3_PENALTY=0.188989 PRIMER_LEFT_3_PENALTY=0.026532 PRIMER_RIGHT_3_PENALTY=0.162457 PRIMER_INTERNAL_3_PENALTY=0.112124 PRIMER_LEFT_3_SEQUENCE=AGCAAACATGGTTGGACCTC PRIMER_RIGHT_3_SEQUENCE=TGGCCATGTGTGAGAACTGT PRIMER_INTERNAL_3_SEQUENCE=CTCACCATGCAAATCACCTG PRIMER_LEFT_3=153,20 PRIMER_RIGHT_3=231,20 PRIMER_INTERNAL_3=189,20 PRIMER_LEFT_3_TM=59.973 PRIMER_RIGHT_3_TM=60.162 PRIMER_INTERNAL_3_TM=60.112 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=0.353715 PRIMER_LEFT_4_PENALTY=0.226618 PRIMER_RIGHT_4_PENALTY=0.127097 PRIMER_INTERNAL_4_PENALTY=0.162457 PRIMER_LEFT_4_SEQUENCE=TCATATACCCCTGGCTCACC PRIMER_RIGHT_4_SEQUENCE=TGCCCACAGACACAGAAAAG PRIMER_INTERNAL_4_SEQUENCE=ACAGTTCTCACACATGGCCA PRIMER_LEFT_4=175,20 PRIMER_RIGHT_4=254,20 PRIMER_INTERNAL_4=212,20 PRIMER_LEFT_4_TM=59.773 PRIMER_RIGHT_4_TM=59.873 PRIMER_INTERNAL_4_TM=60.162 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=7.3000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH157 SEQUENCE_TEMPLATE=AGATTGNCTGCCTGCNGGTCGACTCTAGAGGATCCCCCTCTATTGATACGTGCCTACTTATTCACTTTGGTTTGTGTTTTATGTTGCTGGTATCATCATCTCTGTTTGACTCTTCTCATCTGTCTTGCCCACTGTTCCTAGTTGTCTCTCTCTTTTCTTTCCAGACTTCTAGTTAAGTATCTTATTCTAAGCATCAGATGGTAAAT SEQUENCE_INCLUDED_REGION=36,170 P3_COMMENT=CL is TACGTGCCTACTTATTCACTTTG P3_COMMENT=CR is AGAGACAACTAGGAACAGTGGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.466107 PRIMER_LEFT_0_PENALTY=3.168089 PRIMER_RIGHT_0_PENALTY=0.298018 PRIMER_INTERNAL_0_PENALTY=3.146910 PRIMER_LEFT_0_SEQUENCE=CGTGCCTACTTATTCACTTTGG PRIMER_RIGHT_0_SEQUENCE=AGGAACAGTGGGCAAGACAG PRIMER_INTERNAL_0_SEQUENCE=TGTTGCTGGTATCATCATCTCTG PRIMER_LEFT_0=48,22 PRIMER_RIGHT_0=138,20 PRIMER_INTERNAL_0=81,23 PRIMER_LEFT_0_TM=58.832 PRIMER_RIGHT_0_TM=60.298 PRIMER_INTERNAL_0_TM=60.147 PRIMER_LEFT_0_GC_PERCENT=45.455 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=91 PRIMER_PAIR_1_PENALTY=3.687734 PRIMER_LEFT_1_PENALTY=3.389716 PRIMER_RIGHT_1_PENALTY=0.298018 PRIMER_INTERNAL_1_PENALTY=3.146910 PRIMER_LEFT_1_SEQUENCE=CGTGCCTACTTATTCACTTTGGT PRIMER_RIGHT_1_SEQUENCE=AGGAACAGTGGGCAAGACAG PRIMER_INTERNAL_1_SEQUENCE=TGTTGCTGGTATCATCATCTCTG PRIMER_LEFT_1=48,23 PRIMER_RIGHT_1=138,20 PRIMER_INTERNAL_1=81,23 PRIMER_LEFT_1_TM=59.610 PRIMER_RIGHT_1_TM=60.298 PRIMER_INTERNAL_1_TM=60.147 PRIMER_LEFT_1_GC_PERCENT=43.478 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=43.478 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=3.687734 PRIMER_LEFT_2_PENALTY=3.389716 PRIMER_RIGHT_2_PENALTY=0.298018 PRIMER_INTERNAL_2_PENALTY=3.146910 PRIMER_LEFT_2_SEQUENCE=ACGTGCCTACTTATTCACTTTGG PRIMER_RIGHT_2_SEQUENCE=AGGAACAGTGGGCAAGACAG PRIMER_INTERNAL_2_SEQUENCE=TGTTGCTGGTATCATCATCTCTG PRIMER_LEFT_2=47,23 PRIMER_RIGHT_2=138,20 PRIMER_INTERNAL_2=81,23 PRIMER_LEFT_2_TM=59.610 PRIMER_RIGHT_2_TM=60.298 PRIMER_INTERNAL_2_TM=60.147 PRIMER_LEFT_2_GC_PERCENT=43.478 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.8000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=92 PRIMER_PAIR_3_PENALTY=3.739324 PRIMER_LEFT_3_PENALTY=2.575721 PRIMER_RIGHT_3_PENALTY=1.163603 PRIMER_INTERNAL_3_PENALTY=5.064245 PRIMER_LEFT_3_SEQUENCE=GGTTTGTGTTTTATGTTGCTGG PRIMER_RIGHT_3_SEQUENCE=GACAACTAGGAACAGTGGGCA PRIMER_INTERNAL_3_SEQUENCE=CATCATCTCTGTTTGACTCTTCTCA PRIMER_LEFT_3=68,22 PRIMER_RIGHT_3=145,21 PRIMER_INTERNAL_3=93,25 PRIMER_LEFT_3_TM=59.424 PRIMER_RIGHT_3_TM=60.164 PRIMER_INTERNAL_3_TM=59.936 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=11.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=78 PRIMER_PAIR_4_PENALTY=3.753478 PRIMER_LEFT_4_PENALTY=3.168089 PRIMER_RIGHT_4_PENALTY=0.585389 PRIMER_INTERNAL_4_PENALTY=3.146910 PRIMER_LEFT_4_SEQUENCE=CGTGCCTACTTATTCACTTTGG PRIMER_RIGHT_4_SEQUENCE=CAGTGGGCAAGACAGATGAG PRIMER_INTERNAL_4_SEQUENCE=TGTTGCTGGTATCATCATCTCTG PRIMER_LEFT_4=48,22 PRIMER_RIGHT_4=133,20 PRIMER_INTERNAL_4=81,23 PRIMER_LEFT_4_TM=58.832 PRIMER_RIGHT_4_TM=59.415 PRIMER_INTERNAL_4_TM=60.147 PRIMER_LEFT_4_GC_PERCENT=45.455 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.8000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH159 SEQUENCE_TEMPLATE=CTCCAGGCCAACAGAGCCCCAGATGTGGAAGTAGAGTNNGTTGGACTGCAGTCCCACTGGCTACAATCAAACATGGCAGGCCAGATCTGTTCAGTATTAGTNCTGGNTCCAAAACCTATACATCTAGATGTNTTTCAGAATTCAGAATTATTTAGATTTTGGAAAGGTAATTTGGCATGTATGTGTGTATATTTAATAATACACTGATGGAGTCAGGGGATAGTATGCCATAATCGAACATTATTATCTTCATAGCAAATGTATGA SEQUENCE_INCLUDED_REGION=0,266 P3_COMMENT=CL is CCCACTGGCTACAATCAAAC P3_COMMENT=CR is TTTGCTATGAAGATAATAATGTTCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.490504 PRIMER_LEFT_0_PENALTY=0.072951 PRIMER_RIGHT_0_PENALTY=0.417552 PRIMER_INTERNAL_0_PENALTY=0.141511 PRIMER_LEFT_0_SEQUENCE=GCCCCAGATGTGGAAGTAGA PRIMER_RIGHT_0_SEQUENCE=ACTGAACAGATCTGGCCTGC PRIMER_INTERNAL_0_SEQUENCE=GCAGTCCCACTGGCTACAAT PRIMER_LEFT_0=15,20 PRIMER_RIGHT_0=94,20 PRIMER_INTERNAL_0=47,20 PRIMER_LEFT_0_TM=60.073 PRIMER_RIGHT_0_TM=60.418 PRIMER_INTERNAL_0_TM=60.142 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=5.4000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=0.490504 PRIMER_LEFT_1_PENALTY=0.072951 PRIMER_RIGHT_1_PENALTY=0.417552 PRIMER_INTERNAL_1_PENALTY=0.141511 PRIMER_LEFT_1_SEQUENCE=GAGCCCCAGATGTGGAAGTA PRIMER_RIGHT_1_SEQUENCE=ACTGAACAGATCTGGCCTGC PRIMER_INTERNAL_1_SEQUENCE=GCAGTCCCACTGGCTACAAT PRIMER_LEFT_1=13,20 PRIMER_RIGHT_1=94,20 PRIMER_INTERNAL_1=47,20 PRIMER_LEFT_1_TM=60.073 PRIMER_RIGHT_1_TM=60.418 PRIMER_INTERNAL_1_TM=60.142 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=5.7000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=1.255488 PRIMER_LEFT_2_PENALTY=0.837936 PRIMER_RIGHT_2_PENALTY=0.417552 PRIMER_INTERNAL_2_PENALTY=0.141511 PRIMER_LEFT_2_SEQUENCE=AGCCCCAGATGTGGAAGTAG PRIMER_RIGHT_2_SEQUENCE=ACTGAACAGATCTGGCCTGC PRIMER_INTERNAL_2_SEQUENCE=GCAGTCCCACTGGCTACAAT PRIMER_LEFT_2=14,20 PRIMER_RIGHT_2=94,20 PRIMER_INTERNAL_2=47,20 PRIMER_LEFT_2_TM=59.162 PRIMER_RIGHT_2_TM=60.418 PRIMER_INTERNAL_2_TM=60.142 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=5.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=1.469898 PRIMER_LEFT_3_PENALTY=1.052345 PRIMER_RIGHT_3_PENALTY=0.417552 PRIMER_INTERNAL_3_PENALTY=0.141511 PRIMER_LEFT_3_SEQUENCE=AGAGCCCCAGATGTGGAAGT PRIMER_RIGHT_3_SEQUENCE=ACTGAACAGATCTGGCCTGC PRIMER_INTERNAL_3_SEQUENCE=GCAGTCCCACTGGCTACAAT PRIMER_LEFT_3=12,20 PRIMER_RIGHT_3=94,20 PRIMER_INTERNAL_3=47,20 PRIMER_LEFT_3_TM=61.052 PRIMER_RIGHT_3_TM=60.418 PRIMER_INTERNAL_3_TM=60.142 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=1.481742 PRIMER_LEFT_4_PENALTY=1.064190 PRIMER_RIGHT_4_PENALTY=0.417552 PRIMER_INTERNAL_4_PENALTY=0.141511 PRIMER_LEFT_4_SEQUENCE=GAGCCCCAGATGTGGAAGT PRIMER_RIGHT_4_SEQUENCE=ACTGAACAGATCTGGCCTGC PRIMER_INTERNAL_4_SEQUENCE=GCAGTCCCACTGGCTACAAT PRIMER_LEFT_4=13,19 PRIMER_RIGHT_4=94,20 PRIMER_INTERNAL_4=47,20 PRIMER_LEFT_4_TM=60.064 PRIMER_RIGHT_4_TM=60.418 PRIMER_INTERNAL_4_TM=60.142 PRIMER_LEFT_4_GC_PERCENT=57.895 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH160 SEQUENCE_TEMPLATE=CTGTGGGAAAAATAAGGCCTTGAAGAATGAAAAAAGTTCTATTAAAACAATTCTATTTAGGATCTGGTTACACACATTCATAAAACTAAAGATGTNNGATTCTGATTNGATTGGTAAAGTCAGTTAGNCACAAAGTAAAGAATCAAACTTAAAGGNTACAGGNCAGAGACTGAAGNTCATTGTTAAGANGGCAATGAACACCTTTAGTCTAATAAAG SEQUENCE_INCLUDED_REGION=0,217 P3_COMMENT=CL is TGGGAAAAATAAGGCCTTGA P3_COMMENT=CR is TATTAGACTAAAGGTGTTCATTGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=10.419566 PRIMER_LEFT_0_PENALTY=4.951910 PRIMER_RIGHT_0_PENALTY=5.467656 PRIMER_INTERNAL_0_PENALTY=8.811170 PRIMER_LEFT_0_SEQUENCE=AGGATCTGGTTACACACATTCA PRIMER_RIGHT_0_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC PRIMER_INTERNAL_0_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG PRIMER_LEFT_0=58,22 PRIMER_RIGHT_0=211,23 PRIMER_INTERNAL_0=128,27 PRIMER_LEFT_0_TM=57.048 PRIMER_RIGHT_0_TM=57.532 PRIMER_INTERNAL_0_TM=58.189 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=39.130 PRIMER_INTERNAL_0_GC_PERCENT=29.630 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=10.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=154 PRIMER_PAIR_1_PENALTY=10.646861 PRIMER_LEFT_1_PENALTY=5.179204 PRIMER_RIGHT_1_PENALTY=5.467656 PRIMER_INTERNAL_1_PENALTY=8.811170 PRIMER_LEFT_1_SEQUENCE=TTTAGGATCTGGTTACACACATTCA PRIMER_RIGHT_1_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC PRIMER_INTERNAL_1_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG PRIMER_LEFT_1=55,25 PRIMER_RIGHT_1=211,23 PRIMER_INTERNAL_1=128,27 PRIMER_LEFT_1_TM=59.821 PRIMER_RIGHT_1_TM=57.532 PRIMER_INTERNAL_1_TM=58.189 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=39.130 PRIMER_INTERNAL_1_GC_PERCENT=29.630 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=157 PRIMER_PAIR_2_PENALTY=10.904149 PRIMER_LEFT_2_PENALTY=5.436493 PRIMER_RIGHT_2_PENALTY=5.467656 PRIMER_INTERNAL_2_PENALTY=8.811170 PRIMER_LEFT_2_SEQUENCE=TTAGGATCTGGTTACACACATTCA PRIMER_RIGHT_2_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC PRIMER_INTERNAL_2_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG PRIMER_LEFT_2=56,24 PRIMER_RIGHT_2=211,23 PRIMER_INTERNAL_2=128,27 PRIMER_LEFT_2_TM=58.564 PRIMER_RIGHT_2_TM=57.532 PRIMER_INTERNAL_2_TM=58.189 PRIMER_LEFT_2_GC_PERCENT=37.500 PRIMER_RIGHT_2_GC_PERCENT=39.130 PRIMER_INTERNAL_2_GC_PERCENT=29.630 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=156 PRIMER_PAIR_3_PENALTY=10.995102 PRIMER_LEFT_3_PENALTY=5.527445 PRIMER_RIGHT_3_PENALTY=5.467656 PRIMER_INTERNAL_3_PENALTY=8.811170 PRIMER_LEFT_3_SEQUENCE=AGGATCTGGTTACACACATTCAT PRIMER_RIGHT_3_SEQUENCE=TTAGACTAAAGGTGTTCATTGCC PRIMER_INTERNAL_3_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG PRIMER_LEFT_3=58,23 PRIMER_RIGHT_3=211,23 PRIMER_INTERNAL_3=128,27 PRIMER_LEFT_3_TM=57.473 PRIMER_RIGHT_3_TM=57.532 PRIMER_INTERNAL_3_TM=58.189 PRIMER_LEFT_3_GC_PERCENT=39.130 PRIMER_RIGHT_3_GC_PERCENT=39.130 PRIMER_INTERNAL_3_GC_PERCENT=29.630 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=154 PRIMER_PAIR_4_PENALTY=11.064529 PRIMER_LEFT_4_PENALTY=4.951910 PRIMER_RIGHT_4_PENALTY=6.112620 PRIMER_INTERNAL_4_PENALTY=8.811170 PRIMER_LEFT_4_SEQUENCE=AGGATCTGGTTACACACATTCA PRIMER_RIGHT_4_SEQUENCE=ATTAGACTAAAGGTGTTCATTGCC PRIMER_INTERNAL_4_SEQUENCE=CACAAAGTAAAGAATCAAACTTAAAGG PRIMER_LEFT_4=58,22 PRIMER_RIGHT_4=212,24 PRIMER_INTERNAL_4=128,27 PRIMER_LEFT_4_TM=57.048 PRIMER_RIGHT_4_TM=57.887 PRIMER_INTERNAL_4_TM=58.189 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=37.500 PRIMER_INTERNAL_4_GC_PERCENT=29.630 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=10.0000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=155 = SEQUENCE_ID=MH161 SEQUENCE_TEMPLATE=CTCTTACTAATAGGATGGGAGAGTNNGAAGGAACATCAGAGATTATTTTACAGATAACAAAACTAAGACTCAGATTAAGATGCTTCTTCATGGTTACTGTAGGATTTTAGGCACCTCACTCTTTCCAAATTGCTCTAATTGACAATTCAAACAAAATTCTACCCTCTTGTGCCTCATCCATTCATACATCCTTTCTGTCCACAACAGTGTACTTTATACATGTATATACACCCACCTACATACATATAG SEQUENCE_INCLUDED_REGION=0,249 P3_COMMENT=CL is CTCTTACTAATAGGATGGGAGAGT P3_COMMENT=CR is TACACTGTTGTGGACAGAAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.357046 PRIMER_LEFT_0_PENALTY=0.156061 PRIMER_RIGHT_0_PENALTY=0.200985 PRIMER_INTERNAL_0_PENALTY=5.051029 PRIMER_LEFT_0_SEQUENCE=AGGCACCTCACTCTTTCCAA PRIMER_RIGHT_0_SEQUENCE=TGAATGGATGAGGCACAAGA PRIMER_INTERNAL_0_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA PRIMER_LEFT_0=108,20 PRIMER_RIGHT_0=183,20 PRIMER_INTERNAL_0=130,25 PRIMER_LEFT_0_TM=59.844 PRIMER_RIGHT_0_TM=60.201 PRIMER_INTERNAL_0_TM=60.051 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=28.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=9.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=0.433284 PRIMER_LEFT_1_PENALTY=0.232299 PRIMER_RIGHT_1_PENALTY=0.200985 PRIMER_INTERNAL_1_PENALTY=5.051029 PRIMER_LEFT_1_SEQUENCE=GGCACCTCACTCTTTCCAAA PRIMER_RIGHT_1_SEQUENCE=TGAATGGATGAGGCACAAGA PRIMER_INTERNAL_1_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA PRIMER_LEFT_1=109,20 PRIMER_RIGHT_1=183,20 PRIMER_INTERNAL_1=130,25 PRIMER_LEFT_1_TM=60.232 PRIMER_RIGHT_1_TM=60.201 PRIMER_INTERNAL_1_TM=60.051 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=28.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=9.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=1.212143 PRIMER_LEFT_2_PENALTY=1.139192 PRIMER_RIGHT_2_PENALTY=0.072951 PRIMER_INTERNAL_2_PENALTY=5.051029 PRIMER_LEFT_2_SEQUENCE=TAGGCACCTCACTCTTTCCAA PRIMER_RIGHT_2_SEQUENCE=AATGGATGAGGCACAAGAGG PRIMER_INTERNAL_2_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA PRIMER_LEFT_2=107,21 PRIMER_RIGHT_2=181,20 PRIMER_INTERNAL_2=130,25 PRIMER_LEFT_2_TM=59.861 PRIMER_RIGHT_2_TM=60.073 PRIMER_INTERNAL_2_TM=60.051 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=28.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=9.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=1.212143 PRIMER_LEFT_3_PENALTY=1.139192 PRIMER_RIGHT_3_PENALTY=0.072951 PRIMER_INTERNAL_3_PENALTY=5.051029 PRIMER_LEFT_3_SEQUENCE=TTAGGCACCTCACTCTTTCCA PRIMER_RIGHT_3_SEQUENCE=AATGGATGAGGCACAAGAGG PRIMER_INTERNAL_3_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA PRIMER_LEFT_3=106,21 PRIMER_RIGHT_3=181,20 PRIMER_INTERNAL_3=130,25 PRIMER_LEFT_3_TM=59.861 PRIMER_RIGHT_3_TM=60.073 PRIMER_INTERNAL_3_TM=60.051 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=28.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=9.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=1.216344 PRIMER_LEFT_4_PENALTY=0.156061 PRIMER_RIGHT_4_PENALTY=1.060283 PRIMER_INTERNAL_4_PENALTY=5.051029 PRIMER_LEFT_4_SEQUENCE=AGGCACCTCACTCTTTCCAA PRIMER_RIGHT_4_SEQUENCE=TGTATGAATGGATGAGGCACA PRIMER_INTERNAL_4_SEQUENCE=TGCTCTAATTGACAATTCAAACAAA PRIMER_LEFT_4=108,20 PRIMER_RIGHT_4=187,21 PRIMER_INTERNAL_4=130,25 PRIMER_LEFT_4_TM=59.844 PRIMER_RIGHT_4_TM=59.940 PRIMER_INTERNAL_4_TM=60.051 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=28.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=9.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH162 SEQUENCE_TEMPLATE=ATGGAGATGTTGTCACCTGGCATTATATGCTATAATNCTTGGTCACATTTATAAGTTTTTTTATTCTAATACAATGGATCTGAGTNTCATTAGGAGCCTTGCAGACAAGTGTTTCTGGGTAACTTCTCAGTAACATCTTTGACATTTGTTTAGTTGCAGACCTAAATGGCCACTGATGGATACTGCAGTTTCAGTAAGCAGTGGAGTTTATTTCCCTGG SEQUENCE_INCLUDED_REGION=0,219 P3_COMMENT=CL is AGATGTTGTCACCTGGCATT P3_COMMENT=CR is ACTCCACTGCTTACTGAAACTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.313697 PRIMER_LEFT_0_PENALTY=0.127097 PRIMER_RIGHT_0_PENALTY=0.186600 PRIMER_INTERNAL_0_PENALTY=5.416361 PRIMER_LEFT_0_SEQUENCE=TGCAGACAAGTGTTTCTGGG PRIMER_RIGHT_0_SEQUENCE=CCATCAGTGGCCATTTAGGT PRIMER_INTERNAL_0_SEQUENCE=TCTTTGACATTTGTTTAGTTGCA PRIMER_LEFT_0=99,20 PRIMER_RIGHT_0=178,20 PRIMER_INTERNAL_0=135,23 PRIMER_LEFT_0_TM=59.873 PRIMER_RIGHT_0_TM=59.813 PRIMER_INTERNAL_0_TM=57.584 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=30.435 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=0.639932 PRIMER_LEFT_1_PENALTY=0.453332 PRIMER_RIGHT_1_PENALTY=0.186600 PRIMER_INTERNAL_1_PENALTY=5.416361 PRIMER_LEFT_1_SEQUENCE=GGAGCCTTGCAGACAAGTGT PRIMER_RIGHT_1_SEQUENCE=CCATCAGTGGCCATTTAGGT PRIMER_INTERNAL_1_SEQUENCE=TCTTTGACATTTGTTTAGTTGCA PRIMER_LEFT_1=92,20 PRIMER_RIGHT_1=178,20 PRIMER_INTERNAL_1=135,23 PRIMER_LEFT_1_TM=60.453 PRIMER_RIGHT_1_TM=59.813 PRIMER_INTERNAL_1_TM=57.584 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=30.435 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.1000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=87 PRIMER_PAIR_2_PENALTY=0.775568 PRIMER_LEFT_2_PENALTY=0.588967 PRIMER_RIGHT_2_PENALTY=0.186600 PRIMER_INTERNAL_2_PENALTY=5.416361 PRIMER_LEFT_2_SEQUENCE=AGGAGCCTTGCAGACAAGTG PRIMER_RIGHT_2_SEQUENCE=CCATCAGTGGCCATTTAGGT PRIMER_INTERNAL_2_SEQUENCE=TCTTTGACATTTGTTTAGTTGCA PRIMER_LEFT_2=91,20 PRIMER_RIGHT_2=178,20 PRIMER_INTERNAL_2=135,23 PRIMER_LEFT_2_TM=60.589 PRIMER_RIGHT_2_TM=59.813 PRIMER_INTERNAL_2_TM=57.584 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=30.435 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=88 PRIMER_PAIR_3_PENALTY=0.784190 PRIMER_LEFT_3_PENALTY=0.127097 PRIMER_RIGHT_3_PENALTY=0.657094 PRIMER_INTERNAL_3_PENALTY=7.414202 PRIMER_LEFT_3_SEQUENCE=TGCAGACAAGTGTTTCTGGG PRIMER_RIGHT_3_SEQUENCE=AGTGGCCATTTAGGTCTGCA PRIMER_INTERNAL_3_SEQUENCE=TTCTCAGTAACATCTTTGACATTTG PRIMER_LEFT_3=99,20 PRIMER_RIGHT_3=173,20 PRIMER_INTERNAL_3=123,25 PRIMER_LEFT_3_TM=59.873 PRIMER_RIGHT_3_TM=60.657 PRIMER_INTERNAL_3_TM=57.586 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=32.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=0.784190 PRIMER_LEFT_4_PENALTY=0.127097 PRIMER_RIGHT_4_PENALTY=0.657094 PRIMER_INTERNAL_4_PENALTY=7.414202 PRIMER_LEFT_4_SEQUENCE=TGCAGACAAGTGTTTCTGGG PRIMER_RIGHT_4_SEQUENCE=CAGTGGCCATTTAGGTCTGC PRIMER_INTERNAL_4_SEQUENCE=TTCTCAGTAACATCTTTGACATTTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=174,20 PRIMER_INTERNAL_4=123,25 PRIMER_LEFT_4_TM=59.873 PRIMER_RIGHT_4_TM=60.657 PRIMER_INTERNAL_4_TM=57.586 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=32.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH164 SEQUENCE_TEMPLATE=CTACACCACCCAAAATGAGCAGTTTCTCTAAAGAGATTGGGTTCAGTAAAGGTCAGTCCTACTTCAATTTCAGTCTGGCCCTTCTCATTCTTATGTTCTACTTAAATATTTATCTTCTATTTTCGTACCAAAATTATTAGATGTTTCTTAGTTTACATTAAGTTTCTTTTAACTGGTAACACGTGAGGATTTTCATAGACAATTTTTTCTTTTGAACAAAATTGTCAATATGTCTT SEQUENCE_INCLUDED_REGION=0,236 P3_COMMENT=CL is CTACACCACCCAAAATGAGC P3_COMMENT=CR is AATTGTCTATGAAAATCCTCACG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.226156 PRIMER_LEFT_0_PENALTY=0.029253 PRIMER_RIGHT_0_PENALTY=0.196903 PRIMER_INTERNAL_0_PENALTY=2.394672 PRIMER_LEFT_0_SEQUENCE=CCACCCAAAATGAGCAGTTT PRIMER_RIGHT_0_SEQUENCE=ATGAGAAGGGCCAGACTGAA PRIMER_INTERNAL_0_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC PRIMER_LEFT_0=5,20 PRIMER_RIGHT_0=87,20 PRIMER_INTERNAL_0=37,22 PRIMER_LEFT_0_TM=59.971 PRIMER_RIGHT_0_TM=59.803 PRIMER_INTERNAL_0_TM=60.395 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=83 PRIMER_PAIR_1_PENALTY=0.226156 PRIMER_LEFT_1_PENALTY=0.029253 PRIMER_RIGHT_1_PENALTY=0.196903 PRIMER_INTERNAL_1_PENALTY=2.394672 PRIMER_LEFT_1_SEQUENCE=CCACCCAAAATGAGCAGTTT PRIMER_RIGHT_1_SEQUENCE=AATGAGAAGGGCCAGACTGA PRIMER_INTERNAL_1_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC PRIMER_LEFT_1=5,20 PRIMER_RIGHT_1=88,20 PRIMER_INTERNAL_1=37,22 PRIMER_LEFT_1_TM=59.971 PRIMER_RIGHT_1_TM=59.803 PRIMER_INTERNAL_1_TM=60.395 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=84 PRIMER_PAIR_2_PENALTY=0.226156 PRIMER_LEFT_2_PENALTY=0.029253 PRIMER_RIGHT_2_PENALTY=0.196903 PRIMER_INTERNAL_2_PENALTY=2.394672 PRIMER_LEFT_2_SEQUENCE=CCACCCAAAATGAGCAGTTT PRIMER_RIGHT_2_SEQUENCE=GAATGAGAAGGGCCAGACTG PRIMER_INTERNAL_2_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC PRIMER_LEFT_2=5,20 PRIMER_RIGHT_2=89,20 PRIMER_INTERNAL_2=37,22 PRIMER_LEFT_2_TM=59.971 PRIMER_RIGHT_2_TM=59.803 PRIMER_INTERNAL_2_TM=60.395 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=85 PRIMER_PAIR_3_PENALTY=0.232446 PRIMER_LEFT_3_PENALTY=0.035543 PRIMER_RIGHT_3_PENALTY=0.196903 PRIMER_INTERNAL_3_PENALTY=2.394672 PRIMER_LEFT_3_SEQUENCE=TACACCACCCAAAATGAGCA PRIMER_RIGHT_3_SEQUENCE=ATGAGAAGGGCCAGACTGAA PRIMER_INTERNAL_3_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC PRIMER_LEFT_3=1,20 PRIMER_RIGHT_3=87,20 PRIMER_INTERNAL_3=37,22 PRIMER_LEFT_3_TM=59.964 PRIMER_RIGHT_3_TM=59.803 PRIMER_INTERNAL_3_TM=60.395 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=87 PRIMER_PAIR_4_PENALTY=0.232446 PRIMER_LEFT_4_PENALTY=0.035543 PRIMER_RIGHT_4_PENALTY=0.196903 PRIMER_INTERNAL_4_PENALTY=2.394672 PRIMER_LEFT_4_SEQUENCE=TACACCACCCAAAATGAGCA PRIMER_RIGHT_4_SEQUENCE=AATGAGAAGGGCCAGACTGA PRIMER_INTERNAL_4_SEQUENCE=TGGGTTCAGTAAAGGTCAGTCC PRIMER_LEFT_4=1,20 PRIMER_RIGHT_4=88,20 PRIMER_INTERNAL_4=37,22 PRIMER_LEFT_4_TM=59.964 PRIMER_RIGHT_4_TM=59.803 PRIMER_INTERNAL_4_TM=60.395 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=88 = SEQUENCE_ID=MH168 SEQUENCE_TEMPLATE=CTTAGAAATAGAAGAGGCTTCCTCCCTCTAATGGTTCCACTCAAGTNCCTATCTGGTTCCAAGAGGTCTTCTTTTTGGATTAACAAAAATTAGTTCCAAAAGTGAGAAATTGTGCATCTTGTGGAAATGAGAGAACCACTGGCTATGCCTAGAAAATGTATCCTTGGAGGCACTAGGAAAATGGCAGTAAGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTAT SEQUENCE_INCLUDED_REGION=0,189 P3_COMMENT=CL is GAAATAGAAGAGGCTTCCTCCC P3_COMMENT=CR is CCATTTTCCTAGTGCCTCCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.170026 PRIMER_LEFT_0_PENALTY=1.103702 PRIMER_RIGHT_0_PENALTY=0.066323 PRIMER_INTERNAL_0_PENALTY=0.687142 PRIMER_LEFT_0_SEQUENCE=TTGTGCATCTTGTGGAAATGA PRIMER_RIGHT_0_SEQUENCE=CCATTTTCCTAGTGCCTCCA PRIMER_INTERNAL_0_SEQUENCE=GAGAACCACTGGCTATGCCT PRIMER_LEFT_0=109,21 PRIMER_RIGHT_0=183,20 PRIMER_INTERNAL_0=130,20 PRIMER_LEFT_0_TM=60.104 PRIMER_RIGHT_0_TM=60.066 PRIMER_INTERNAL_0_TM=59.313 PRIMER_LEFT_0_GC_PERCENT=38.095 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=1.317208 PRIMER_LEFT_1_PENALTY=1.103702 PRIMER_RIGHT_1_PENALTY=0.213506 PRIMER_INTERNAL_1_PENALTY=0.687142 PRIMER_LEFT_1_SEQUENCE=TTGTGCATCTTGTGGAAATGA PRIMER_RIGHT_1_SEQUENCE=TGCCATTTTCCTAGTGCCTC PRIMER_INTERNAL_1_SEQUENCE=GAGAACCACTGGCTATGCCT PRIMER_LEFT_1=109,21 PRIMER_RIGHT_1=185,20 PRIMER_INTERNAL_1=130,20 PRIMER_LEFT_1_TM=60.104 PRIMER_RIGHT_1_TM=60.214 PRIMER_INTERNAL_1_TM=59.313 PRIMER_LEFT_1_GC_PERCENT=38.095 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=1.506940 PRIMER_LEFT_2_PENALTY=1.293434 PRIMER_RIGHT_2_PENALTY=0.213506 PRIMER_INTERNAL_2_PENALTY=1.629732 PRIMER_LEFT_2_SEQUENCE=TGTGCATCTTGTGGAAATGAG PRIMER_RIGHT_2_SEQUENCE=TGCCATTTTCCTAGTGCCTC PRIMER_INTERNAL_2_SEQUENCE=CCACTGGCTATGCCTAGAAAA PRIMER_LEFT_2=110,21 PRIMER_RIGHT_2=185,20 PRIMER_INTERNAL_2=135,21 PRIMER_LEFT_2_TM=59.707 PRIMER_RIGHT_2_TM=60.214 PRIMER_INTERNAL_2_TM=59.370 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=76 PRIMER_PAIR_3_PENALTY=1.593388 PRIMER_LEFT_3_PENALTY=1.379883 PRIMER_RIGHT_3_PENALTY=0.213506 PRIMER_INTERNAL_3_PENALTY=0.687142 PRIMER_LEFT_3_SEQUENCE=TGTGCATCTTGTGGAAATGA PRIMER_RIGHT_3_SEQUENCE=TGCCATTTTCCTAGTGCCTC PRIMER_INTERNAL_3_SEQUENCE=GAGAACCACTGGCTATGCCT PRIMER_LEFT_3=110,20 PRIMER_RIGHT_3=185,20 PRIMER_INTERNAL_3=130,20 PRIMER_LEFT_3_TM=58.620 PRIMER_RIGHT_3_TM=60.214 PRIMER_INTERNAL_3_TM=59.313 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=1.768027 PRIMER_LEFT_4_PENALTY=1.103702 PRIMER_RIGHT_4_PENALTY=0.664324 PRIMER_INTERNAL_4_PENALTY=0.687142 PRIMER_LEFT_4_SEQUENCE=TTGTGCATCTTGTGGAAATGA PRIMER_RIGHT_4_SEQUENCE=CTGCCATTTTCCTAGTGCCT PRIMER_INTERNAL_4_SEQUENCE=GAGAACCACTGGCTATGCCT PRIMER_LEFT_4=109,21 PRIMER_RIGHT_4=186,20 PRIMER_INTERNAL_4=130,20 PRIMER_LEFT_4_TM=60.104 PRIMER_RIGHT_4_TM=59.336 PRIMER_INTERNAL_4_TM=59.313 PRIMER_LEFT_4_GC_PERCENT=38.095 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=78 = SEQUENCE_ID=MH170 SEQUENCE_TEMPLATE=CTCGATCTTCAGAAAGTGACTCTATCAAATCTGAAGGACACCCAAATTTAAAAAAGTATCATGTAATTAAAAAAACCACTTTGCATGAATGTATCTTTTNCACTTTAAAAAATTAAAAAAAATAGTACANNGCAGTACANACCTTTATCCCGTTCTTGTTGCTGCATTGTACTTTTCAACTTATCACTAAGNTCATTTATTATGTTTTCTTTTCTCATTTTCTCTCTTGTTAAAACAGTGTTAAAATTGG SEQUENCE_INCLUDED_REGION=0,250 P3_COMMENT=CL is CTATCAAATCTGAAGGACACCC P3_COMMENT=CR is CACTGTTTTAACAAGAGAGAAAATG P3_COMMENT=CM is CATGTAATTAAAAAAACCACTTTGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=8.866629 PRIMER_LEFT_0_PENALTY=5.899214 PRIMER_RIGHT_0_PENALTY=2.967416 PRIMER_INTERNAL_0_PENALTY=2.917484 PRIMER_LEFT_0_SEQUENCE=CGATCTTCAGAAAGTGACTCTATCA PRIMER_RIGHT_0_SEQUENCE=CATTCATGCAAAGTGGTTTTTT PRIMER_INTERNAL_0_SEQUENCE=TCTGAAGGACACCCAAATTT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=90,22 PRIMER_INTERNAL_0=29,20 PRIMER_LEFT_0_TM=59.101 PRIMER_RIGHT_0_TM=59.033 PRIMER_INTERNAL_0_TM=57.083 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=31.818 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=89 PRIMER_PAIR_1_PENALTY=8.958627 PRIMER_LEFT_1_PENALTY=5.991211 PRIMER_RIGHT_1_PENALTY=2.967416 PRIMER_INTERNAL_1_PENALTY=8.915111 PRIMER_LEFT_1_SEQUENCE=TGACTCTATCAAATCTGAAGGACA PRIMER_RIGHT_1_SEQUENCE=CATTCATGCAAAGTGGTTTTTT PRIMER_INTERNAL_1_SEQUENCE=CCCAAATTTAAAAAAGTATCATGTAA PRIMER_LEFT_1=16,24 PRIMER_RIGHT_1=90,22 PRIMER_INTERNAL_1=40,26 PRIMER_LEFT_1_TM=58.009 PRIMER_RIGHT_1_TM=59.033 PRIMER_INTERNAL_1_TM=57.085 PRIMER_LEFT_1_GC_PERCENT=37.500 PRIMER_RIGHT_1_GC_PERCENT=31.818 PRIMER_INTERNAL_1_GC_PERCENT=23.077 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=9.093109 PRIMER_LEFT_2_PENALTY=5.899214 PRIMER_RIGHT_2_PENALTY=3.193896 PRIMER_INTERNAL_2_PENALTY=2.917484 PRIMER_LEFT_2_SEQUENCE=CGATCTTCAGAAAGTGACTCTATCA PRIMER_RIGHT_2_SEQUENCE=ACATTCATGCAAAGTGGTTTTTT PRIMER_INTERNAL_2_SEQUENCE=TCTGAAGGACACCCAAATTT PRIMER_LEFT_2=2,25 PRIMER_RIGHT_2=91,23 PRIMER_INTERNAL_2=29,20 PRIMER_LEFT_2_TM=59.101 PRIMER_RIGHT_2_TM=59.806 PRIMER_INTERNAL_2_TM=57.083 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=30.435 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=5.9000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=9.127334 PRIMER_LEFT_3_PENALTY=6.159919 PRIMER_RIGHT_3_PENALTY=2.967416 PRIMER_INTERNAL_3_PENALTY=8.915111 PRIMER_LEFT_3_SEQUENCE=GTGACTCTATCAAATCTGAAGGACA PRIMER_RIGHT_3_SEQUENCE=CATTCATGCAAAGTGGTTTTTT PRIMER_INTERNAL_3_SEQUENCE=CCCAAATTTAAAAAAGTATCATGTAA PRIMER_LEFT_3=15,25 PRIMER_RIGHT_3=90,22 PRIMER_INTERNAL_3=40,26 PRIMER_LEFT_3_TM=58.840 PRIMER_RIGHT_3_TM=59.033 PRIMER_INTERNAL_3_TM=57.085 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=31.818 PRIMER_INTERNAL_3_GC_PERCENT=23.077 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=9.156599 PRIMER_LEFT_4_PENALTY=5.899214 PRIMER_RIGHT_4_PENALTY=3.257385 PRIMER_INTERNAL_4_PENALTY=2.917484 PRIMER_LEFT_4_SEQUENCE=CGATCTTCAGAAAGTGACTCTATCA PRIMER_RIGHT_4_SEQUENCE=CATTCATGCAAAGTGGTTTTTTT PRIMER_INTERNAL_4_SEQUENCE=TCTGAAGGACACCCAAATTT PRIMER_LEFT_4=2,25 PRIMER_RIGHT_4=90,23 PRIMER_INTERNAL_4=29,20 PRIMER_LEFT_4_TM=59.101 PRIMER_RIGHT_4_TM=60.257 PRIMER_INTERNAL_4_TM=57.083 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=30.435 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=5.9000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH176 SEQUENCE_TEMPLATE=CTGGTATAAAGGCCCCTTTGGATGANATTNACCCCAACCAGACTAATGGGAGTNCAGAAGGGATGCTGTGGGTCCCCCACATCNTCAGAATGCCAACGGGACCTGGGGGTCCTGTTAATCTTTTTAGCCAGACTGAATTCCATTCTGTTCTTATAAAATCCC SEQUENCE_INCLUDED_REGION=0,162 P3_COMMENT=CL is CTGGTATAAAGGCCCCTTTG P3_COMMENT=CR is AAGATTAACAGGACCCCCAG P3_COMMENT=CM is ACCCCAACCAGACTAATGGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.651395 PRIMER_LEFT_0_PENALTY=0.599485 PRIMER_RIGHT_0_PENALTY=0.051910 PRIMER_INTERNAL_0_PENALTY=1.064190 PRIMER_LEFT_0_SEQUENCE=CCCAACCAGACTAATGGGAG PRIMER_RIGHT_0_SEQUENCE=ATTAACAGGACCCCCAGGTC PRIMER_INTERNAL_0_SEQUENCE=AGAAGGGATGCTGTGGGTC PRIMER_LEFT_0=32,20 PRIMER_RIGHT_0=118,20 PRIMER_INTERNAL_0=55,19 PRIMER_LEFT_0_TM=59.401 PRIMER_RIGHT_0_TM=60.052 PRIMER_INTERNAL_0_TM=60.064 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=87 PRIMER_PAIR_1_PENALTY=0.651395 PRIMER_LEFT_1_PENALTY=0.599485 PRIMER_RIGHT_1_PENALTY=0.051910 PRIMER_INTERNAL_1_PENALTY=1.064190 PRIMER_LEFT_1_SEQUENCE=CCCAACCAGACTAATGGGAG PRIMER_RIGHT_1_SEQUENCE=GATTAACAGGACCCCCAGGT PRIMER_INTERNAL_1_SEQUENCE=AGAAGGGATGCTGTGGGTC PRIMER_LEFT_1=32,20 PRIMER_RIGHT_1=119,20 PRIMER_INTERNAL_1=55,19 PRIMER_LEFT_1_TM=59.401 PRIMER_RIGHT_1_TM=60.052 PRIMER_INTERNAL_1_TM=60.064 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=88 PRIMER_PAIR_2_PENALTY=0.782143 PRIMER_LEFT_2_PENALTY=0.599485 PRIMER_RIGHT_2_PENALTY=0.182658 PRIMER_INTERNAL_2_PENALTY=1.064190 PRIMER_LEFT_2_SEQUENCE=CCCAACCAGACTAATGGGAG PRIMER_RIGHT_2_SEQUENCE=AGATTAACAGGACCCCCAGG PRIMER_INTERNAL_2_SEQUENCE=AGAAGGGATGCTGTGGGTC PRIMER_LEFT_2=32,20 PRIMER_RIGHT_2=120,20 PRIMER_INTERNAL_2=55,19 PRIMER_LEFT_2_TM=59.401 PRIMER_RIGHT_2_TM=60.183 PRIMER_INTERNAL_2_TM=60.064 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=89 PRIMER_PAIR_3_PENALTY=1.039931 PRIMER_LEFT_3_PENALTY=0.988020 PRIMER_RIGHT_3_PENALTY=0.051910 PRIMER_INTERNAL_3_PENALTY=1.064190 PRIMER_LEFT_3_SEQUENCE=ACCCCAACCAGACTAATGGG PRIMER_RIGHT_3_SEQUENCE=ATTAACAGGACCCCCAGGTC PRIMER_INTERNAL_3_SEQUENCE=AGAAGGGATGCTGTGGGTC PRIMER_LEFT_3=30,20 PRIMER_RIGHT_3=118,20 PRIMER_INTERNAL_3=55,19 PRIMER_LEFT_3_TM=60.988 PRIMER_RIGHT_3_TM=60.052 PRIMER_INTERNAL_3_TM=60.064 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=57.895 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.6000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=89 PRIMER_PAIR_4_PENALTY=1.039931 PRIMER_LEFT_4_PENALTY=0.988020 PRIMER_RIGHT_4_PENALTY=0.051910 PRIMER_INTERNAL_4_PENALTY=1.064190 PRIMER_LEFT_4_SEQUENCE=ACCCCAACCAGACTAATGGG PRIMER_RIGHT_4_SEQUENCE=GATTAACAGGACCCCCAGGT PRIMER_INTERNAL_4_SEQUENCE=AGAAGGGATGCTGTGGGTC PRIMER_LEFT_4=30,20 PRIMER_RIGHT_4=119,20 PRIMER_INTERNAL_4=55,19 PRIMER_LEFT_4_TM=60.988 PRIMER_RIGHT_4_TM=60.052 PRIMER_INTERNAL_4_TM=60.064 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=57.895 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.6000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=90 = SEQUENCE_ID=MH177 SEQUENCE_TEMPLATE=CTTTAGATATATTTAAATCATTTGCCATCAGAAGTCTGTACTGTAGGTCTTACTATTATCCTCATTTTACAGATGAAGCACAAAGATCCATAGTCACATATTTTGTAACAAGAGCAGGATCTGCAGCCAGGCAGTCTGGGTCCATGGTCCATGTTCTTACCCATGGTGCAATACTGACTCTTGAAGTCCAGGGTCTACTAAGAATCCAGGTCTACCTGACTCCAAAG SEQUENCE_INCLUDED_REGION=0,227 P3_COMMENT=CL is CATTTGCCATCAGAAGTCTG P3_COMMENT=CR is TGGAGTCAGGTAGACCTGGA P3_COMMENT=CM is ATTATCCTCATTTTACAGATGAAGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.657623 PRIMER_LEFT_0_PENALTY=0.363753 PRIMER_RIGHT_0_PENALTY=1.293870 PRIMER_INTERNAL_0_PENALTY=1.390393 PRIMER_LEFT_0_SEQUENCE=GGTCCATGGTCCATGTTCTT PRIMER_RIGHT_0_SEQUENCE=TTGGAGTCAGGTAGACCTGGA PRIMER_INTERNAL_0_SEQUENCE=CCCATGGTGCAATACTGACTC PRIMER_LEFT_0=138,20 PRIMER_RIGHT_0=224,21 PRIMER_INTERNAL_0=159,21 PRIMER_LEFT_0_TM=59.636 PRIMER_RIGHT_0_TM=59.706 PRIMER_INTERNAL_0_TM=60.390 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_INTERNAL_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=87 PRIMER_PAIR_1_PENALTY=1.842081 PRIMER_LEFT_1_PENALTY=0.035543 PRIMER_RIGHT_1_PENALTY=1.806538 PRIMER_INTERNAL_1_PENALTY=1.710351 PRIMER_LEFT_1_SEQUENCE=TGTTCTTACCCATGGTGCAA PRIMER_RIGHT_1_SEQUENCE=TTTGGAGTCAGGTAGACCTGG PRIMER_INTERNAL_1_SEQUENCE=TGACTCTTGAAGTCCAGGGTC PRIMER_LEFT_1=151,20 PRIMER_RIGHT_1=225,21 PRIMER_INTERNAL_1=174,21 PRIMER_LEFT_1_TM=59.964 PRIMER_RIGHT_1_TM=59.193 PRIMER_INTERNAL_1_TM=59.290 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=52.381 PRIMER_INTERNAL_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=8.00 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=2.147662 PRIMER_LEFT_2_PENALTY=0.363753 PRIMER_RIGHT_2_PENALTY=1.783908 PRIMER_INTERNAL_2_PENALTY=1.390393 PRIMER_LEFT_2_SEQUENCE=GGTCCATGGTCCATGTTCTT PRIMER_RIGHT_2_SEQUENCE=TGGAGTCAGGTAGACCTGGA PRIMER_INTERNAL_2_SEQUENCE=CCCATGGTGCAATACTGACTC PRIMER_LEFT_2=138,20 PRIMER_RIGHT_2=223,20 PRIMER_INTERNAL_2=159,21 PRIMER_LEFT_2_TM=59.636 PRIMER_RIGHT_2_TM=58.216 PRIMER_INTERNAL_2_TM=60.390 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=9.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=86 PRIMER_PAIR_3_PENALTY=2.170291 PRIMER_LEFT_3_PENALTY=0.363753 PRIMER_RIGHT_3_PENALTY=1.806538 PRIMER_INTERNAL_3_PENALTY=1.390393 PRIMER_LEFT_3_SEQUENCE=GGTCCATGGTCCATGTTCTT PRIMER_RIGHT_3_SEQUENCE=TTTGGAGTCAGGTAGACCTGG PRIMER_INTERNAL_3_SEQUENCE=CCCATGGTGCAATACTGACTC PRIMER_LEFT_3=138,20 PRIMER_RIGHT_3=225,21 PRIMER_INTERNAL_3=159,21 PRIMER_LEFT_3_TM=59.636 PRIMER_RIGHT_3_TM=59.193 PRIMER_INTERNAL_3_TM=60.390 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_INTERNAL_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=9.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=88 PRIMER_PAIR_4_PENALTY=2.195208 PRIMER_LEFT_4_PENALTY=0.035543 PRIMER_RIGHT_4_PENALTY=2.159665 PRIMER_INTERNAL_4_PENALTY=1.710351 PRIMER_LEFT_4_SEQUENCE=TGTTCTTACCCATGGTGCAA PRIMER_RIGHT_4_SEQUENCE=CTTTGGAGTCAGGTAGACCTGG PRIMER_INTERNAL_4_SEQUENCE=TGACTCTTGAAGTCCAGGGTC PRIMER_LEFT_4=151,20 PRIMER_RIGHT_4=226,22 PRIMER_INTERNAL_4=174,21 PRIMER_LEFT_4_TM=59.964 PRIMER_RIGHT_4_TM=60.160 PRIMER_INTERNAL_4_TM=59.290 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=54.545 PRIMER_INTERNAL_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=8.00 PRIMER_LEFT_4_END_STABILITY=8.8000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH184 SEQUENCE_TEMPLATE=CTACATTTCAAACCGGAATCATTGGCTCAGTNTGGATTCTCCAGGAAGCAGGCCTGAGGCAAAGGCTGATGTGCTAACACATCAATAGAGTNTATAGTCTTAGGGGCAACAATAGGGAATGAGGTGAGAAAGACAGGTAAGGAGACAGACTCAATAGGAAGATGTGTCATCAAGCAGGACACT SEQUENCE_INCLUDED_REGION=0,183 P3_COMMENT=CL is CAAAGGCTGATGTGCTAACA P3_COMMENT=CR is GTGTCCTGCTTGATGACACA P3_COMMENT=CM is TATAGTCTTAGGGGCAACAATAGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.692117 PRIMER_LEFT_0_PENALTY=1.098301 PRIMER_RIGHT_0_PENALTY=0.593816 PRIMER_INTERNAL_0_PENALTY=0.934426 PRIMER_LEFT_0_SEQUENCE=GATTCTCCAGGAAGCAGGC PRIMER_RIGHT_0_SEQUENCE=TTCCCTATTGTTGCCCCTAA PRIMER_INTERNAL_0_SEQUENCE=AGGCAAAGGCTGATGTGCTA PRIMER_LEFT_0=34,19 PRIMER_RIGHT_0=118,20 PRIMER_INTERNAL_0=56,20 PRIMER_LEFT_0_TM=59.902 PRIMER_RIGHT_0_TM=59.406 PRIMER_INTERNAL_0_TM=60.934 PRIMER_LEFT_0_GC_PERCENT=57.895 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=85 PRIMER_PAIR_1_PENALTY=1.711520 PRIMER_LEFT_1_PENALTY=1.098301 PRIMER_RIGHT_1_PENALTY=0.613219 PRIMER_INTERNAL_1_PENALTY=0.934426 PRIMER_LEFT_1_SEQUENCE=GATTCTCCAGGAAGCAGGC PRIMER_RIGHT_1_SEQUENCE=CTCATTCCCTATTGTTGCCC PRIMER_INTERNAL_1_SEQUENCE=AGGCAAAGGCTGATGTGCTA PRIMER_LEFT_1=34,19 PRIMER_RIGHT_1=122,20 PRIMER_INTERNAL_1=56,20 PRIMER_LEFT_1_TM=59.902 PRIMER_RIGHT_1_TM=59.387 PRIMER_INTERNAL_1_TM=60.934 PRIMER_LEFT_1_GC_PERCENT=57.895 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=11.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=89 PRIMER_PAIR_2_PENALTY=1.711520 PRIMER_LEFT_2_PENALTY=1.098301 PRIMER_RIGHT_2_PENALTY=0.613219 PRIMER_INTERNAL_2_PENALTY=0.934426 PRIMER_LEFT_2_SEQUENCE=GATTCTCCAGGAAGCAGGC PRIMER_RIGHT_2_SEQUENCE=CCTCATTCCCTATTGTTGCC PRIMER_INTERNAL_2_SEQUENCE=AGGCAAAGGCTGATGTGCTA PRIMER_LEFT_2=34,19 PRIMER_RIGHT_2=123,20 PRIMER_INTERNAL_2=56,20 PRIMER_LEFT_2_TM=59.902 PRIMER_RIGHT_2_TM=59.387 PRIMER_INTERNAL_2_TM=60.934 PRIMER_LEFT_2_GC_PERCENT=57.895 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=1.847639 PRIMER_LEFT_3_PENALTY=1.253823 PRIMER_RIGHT_3_PENALTY=0.593816 PRIMER_INTERNAL_3_PENALTY=0.934426 PRIMER_LEFT_3_SEQUENCE=GGATTCTCCAGGAAGCAGG PRIMER_RIGHT_3_SEQUENCE=TTCCCTATTGTTGCCCCTAA PRIMER_INTERNAL_3_SEQUENCE=AGGCAAAGGCTGATGTGCTA PRIMER_LEFT_3=33,19 PRIMER_RIGHT_3=118,20 PRIMER_INTERNAL_3=56,20 PRIMER_LEFT_3_TM=59.746 PRIMER_RIGHT_3_TM=59.406 PRIMER_INTERNAL_3_TM=60.934 PRIMER_LEFT_3_GC_PERCENT=57.895 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=7.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=86 PRIMER_PAIR_4_PENALTY=1.867041 PRIMER_LEFT_4_PENALTY=1.253823 PRIMER_RIGHT_4_PENALTY=0.613219 PRIMER_INTERNAL_4_PENALTY=0.934426 PRIMER_LEFT_4_SEQUENCE=GGATTCTCCAGGAAGCAGG PRIMER_RIGHT_4_SEQUENCE=CTCATTCCCTATTGTTGCCC PRIMER_INTERNAL_4_SEQUENCE=AGGCAAAGGCTGATGTGCTA PRIMER_LEFT_4=33,19 PRIMER_RIGHT_4=122,20 PRIMER_INTERNAL_4=56,20 PRIMER_LEFT_4_TM=59.746 PRIMER_RIGHT_4_TM=59.387 PRIMER_INTERNAL_4_TM=60.934 PRIMER_LEFT_4_GC_PERCENT=57.895 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=11.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=90 = SEQUENCE_ID=MH192 SEQUENCE_TEMPLATE=AGTGGCCTGATGATGGCTCACTGTAACGTAACTCCTGGCCTCAGCCTCCAGAGTNNCTGGAACTATAGGTACACGCCAACACACCAGGGTAATTTTTTTTTTTTTTTTTTGTAGAGACAGGGTCTCGCTATGTTGCCCACTCTGTTCTATTTTGAAGGTACAACCAACAAGCTTTCTTGACAGNTTGAAAGTGAAAATGAGAGTATGAGAGGAGTTATGGATGATATAAAGTTTTTGGCCTGAGTAACTAGAAATCCTCGCCATCAACCTAGAATGGGGAAGACCATACATTACTTACAGGTTCG SEQUENCE_INCLUDED_REGION=0,305 P3_COMMENT=CL is GCTATGTTGCCCACTCTGTT P3_COMMENT=CR is ATGGTCTTCCCCATTCTAGG P3_COMMENT=CM is TATTTTGAAGGTACAACCAACAAG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.795044 PRIMER_LEFT_0_PENALTY=0.509923 PRIMER_RIGHT_0_PENALTY=0.285121 PRIMER_INTERNAL_0_PENALTY=7.399766 PRIMER_LEFT_0_SEQUENCE=TAGGTACACGCCAACACACC PRIMER_RIGHT_0_SEQUENCE=AGTGGGCAACATAGCGAGAC PRIMER_INTERNAL_0_SEQUENCE=TTTTTTTTTTTTTTTTGTAGAGACAGG PRIMER_LEFT_0=65,20 PRIMER_RIGHT_0=140,20 PRIMER_INTERNAL_0=94,27 PRIMER_LEFT_0_TM=59.490 PRIMER_RIGHT_0_TM=60.285 PRIMER_INTERNAL_0_TM=59.600 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=22.222 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=0.928609 PRIMER_LEFT_1_PENALTY=0.509923 PRIMER_RIGHT_1_PENALTY=0.418685 PRIMER_INTERNAL_1_PENALTY=5.782983 PRIMER_LEFT_1_SEQUENCE=TAGGTACACGCCAACACACC PRIMER_RIGHT_1_SEQUENCE=AGAGTGGGCAACATAGCGAG PRIMER_INTERNAL_1_SEQUENCE=TTTTTTTTTTTTTGTAGAGACAGGG PRIMER_LEFT_1=65,20 PRIMER_RIGHT_1=142,20 PRIMER_INTERNAL_1=97,25 PRIMER_LEFT_1_TM=59.490 PRIMER_RIGHT_1_TM=60.419 PRIMER_INTERNAL_1_TM=59.217 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=28.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=9.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=1.490526 PRIMER_LEFT_2_PENALTY=1.205406 PRIMER_RIGHT_2_PENALTY=0.285121 PRIMER_INTERNAL_2_PENALTY=7.399766 PRIMER_LEFT_2_SEQUENCE=ATAGGTACACGCCAACACACC PRIMER_RIGHT_2_SEQUENCE=AGTGGGCAACATAGCGAGAC PRIMER_INTERNAL_2_SEQUENCE=TTTTTTTTTTTTTTTTGTAGAGACAGG PRIMER_LEFT_2=64,21 PRIMER_RIGHT_2=140,20 PRIMER_INTERNAL_2=94,27 PRIMER_LEFT_2_TM=59.795 PRIMER_RIGHT_2_TM=60.285 PRIMER_INTERNAL_2_TM=59.600 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=22.222 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.6000 PRIMER_RIGHT_2_END_STABILITY=6.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=1.546608 PRIMER_LEFT_3_PENALTY=1.263740 PRIMER_RIGHT_3_PENALTY=0.282868 PRIMER_INTERNAL_3_PENALTY=0.647368 PRIMER_LEFT_3_SEQUENCE=GCCTGATGATGGCTCACTGT PRIMER_RIGHT_3_SEQUENCE=AATTACCCTGGTGTGTTGGC PRIMER_INTERNAL_3_SEQUENCE=AACGTAACTCCTGGCCTCAG PRIMER_LEFT_3=4,20 PRIMER_RIGHT_3=93,20 PRIMER_INTERNAL_3=24,20 PRIMER_LEFT_3_TM=61.264 PRIMER_RIGHT_3_TM=59.717 PRIMER_INTERNAL_3_TM=59.353 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.1000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=1.551977 PRIMER_LEFT_4_PENALTY=1.269110 PRIMER_RIGHT_4_PENALTY=0.282868 PRIMER_INTERNAL_4_PENALTY=1.041745 PRIMER_LEFT_4_SEQUENCE=TGATGATGGCTCACTGTAACG PRIMER_RIGHT_4_SEQUENCE=AATTACCCTGGTGTGTTGGC PRIMER_INTERNAL_4_SEQUENCE=TAACTCCTGGCCTCAGCCT PRIMER_LEFT_4=7,21 PRIMER_RIGHT_4=93,20 PRIMER_INTERNAL_4=28,19 PRIMER_LEFT_4_TM=59.731 PRIMER_RIGHT_4_TM=59.717 PRIMER_INTERNAL_4_TM=59.958 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=57.895 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.7000 PRIMER_RIGHT_4_END_STABILITY=10.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH193 SEQUENCE_TEMPLATE=CTACCACGCCCTGCTCTCCATTCACTCTTGAACTTACTCCAAGCAGACATTTGTCCCCGCCATTCCACAAATAACCTTCATGTAGCCAACTCCAGGAGTNAATACTTTTCCTAAGTCTGTTTACTGGATTTCATCCTTTTTTCAACCTCTAAAAATTTCAGTGCCCTGGGATCTCTGGGTNTCTTTCCCTTGACAATTTCCTCTAATGCTGTGGCTTTTTAGACCACCTTTATGATGATGACATCAAAATTCCTATCCCACTTCTCCTCTGAGGTCCAGTCCCCAATCCCCACTGGAATGTTTAATAGATATATTA SEQUENCE_INCLUDED_REGION=0,316 P3_COMMENT=CL is CTACCACGCCCTGCTCTC P3_COMMENT=CR is TAAACATTCCAGTGGGGATT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.889487 PRIMER_LEFT_0_PENALTY=0.536418 PRIMER_RIGHT_0_PENALTY=0.353069 PRIMER_INTERNAL_0_PENALTY=0.648069 PRIMER_LEFT_0_SEQUENCE=TGCTGTGGCTTTTTAGACCA PRIMER_RIGHT_0_SEQUENCE=AAACATTCCAGTGGGGATTG PRIMER_INTERNAL_0_SEQUENCE=TCTCCTCTGAGGTCCAGTCC PRIMER_LEFT_0=206,20 PRIMER_RIGHT_0=302,20 PRIMER_INTERNAL_0=262,20 PRIMER_LEFT_0_TM=59.464 PRIMER_RIGHT_0_TM=59.647 PRIMER_INTERNAL_0_TM=59.352 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=97 PRIMER_PAIR_1_PENALTY=1.184487 PRIMER_LEFT_1_PENALTY=0.536418 PRIMER_RIGHT_1_PENALTY=0.648069 PRIMER_INTERNAL_1_PENALTY=2.726686 PRIMER_LEFT_1_SEQUENCE=TGCTGTGGCTTTTTAGACCA PRIMER_RIGHT_1_SEQUENCE=GGACTGGACCTCAGAGGAGA PRIMER_INTERNAL_1_SEQUENCE=TGACATCAAAATTCCTATCCCA PRIMER_LEFT_1=206,20 PRIMER_RIGHT_1=281,20 PRIMER_INTERNAL_1=238,22 PRIMER_LEFT_1_TM=59.464 PRIMER_RIGHT_1_TM=59.352 PRIMER_INTERNAL_1_TM=59.273 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=36.364 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=1.354362 PRIMER_LEFT_2_PENALTY=0.595208 PRIMER_RIGHT_2_PENALTY=0.759154 PRIMER_INTERNAL_2_PENALTY=0.177008 PRIMER_LEFT_2_SEQUENCE=CCCTGCTCTCCATTCACTCT PRIMER_RIGHT_2_SEQUENCE=TCCTGGAGTTGGCTACATGA PRIMER_INTERNAL_2_SEQUENCE=CGCCATTCCACAAATAACCT PRIMER_LEFT_2=8,20 PRIMER_RIGHT_2=96,20 PRIMER_INTERNAL_2=57,20 PRIMER_LEFT_2_TM=59.405 PRIMER_RIGHT_2_TM=59.241 PRIMER_INTERNAL_2_TM=59.823 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.1000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=89 PRIMER_PAIR_3_PENALTY=1.451852 PRIMER_LEFT_3_PENALTY=0.759154 PRIMER_RIGHT_3_PENALTY=0.692698 PRIMER_INTERNAL_3_PENALTY=3.156444 PRIMER_LEFT_3_SEQUENCE=TCATGTAGCCAACTCCAGGA PRIMER_RIGHT_3_SEQUENCE=ATCCCAGGGCACTGAAATTT PRIMER_INTERNAL_3_SEQUENCE=GGATTTCATCCTTTTTTCAACC PRIMER_LEFT_3=77,20 PRIMER_RIGHT_3=171,20 PRIMER_INTERNAL_3=125,22 PRIMER_LEFT_3_TM=59.241 PRIMER_RIGHT_3_TM=60.693 PRIMER_INTERNAL_3_TM=58.844 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=36.364 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=95 PRIMER_PAIR_4_PENALTY=1.592521 PRIMER_LEFT_4_PENALTY=1.239452 PRIMER_RIGHT_4_PENALTY=0.353069 PRIMER_INTERNAL_4_PENALTY=0.648069 PRIMER_LEFT_4_SEQUENCE=ATGCTGTGGCTTTTTAGACCA PRIMER_RIGHT_4_SEQUENCE=AAACATTCCAGTGGGGATTG PRIMER_INTERNAL_4_SEQUENCE=TCTCCTCTGAGGTCCAGTCC PRIMER_LEFT_4=205,21 PRIMER_RIGHT_4=302,20 PRIMER_INTERNAL_4=262,20 PRIMER_LEFT_4_TM=59.761 PRIMER_RIGHT_4_TM=59.647 PRIMER_INTERNAL_4_TM=59.352 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH203 SEQUENCE_TEMPLATE=TGAATAACCAATCCACTCCGCTTTTAGTNGGAGTTTTAGTTGGTTAGTTTTGTCAAACCCACAATAAAACCCTGGGGTGGACCAAGAGAGTNNGCCACTCTAGTCCTTGGCTTTCTATATAGCAGGAGGTGCAGACCTTGGAATCTGGAGGAGTCTCTGGTGGCCCTCCCTTGGCCTTTAAATTTCACCCCACCCTCTCCCTCTCCTGAGGCTTTCTGGGCCTCATCTCTGTGCTGAATGCAAAAATGACCACACTTGGCATTCAAGACTCTTCATGACTTCGCTCCTGCTTACTTCTCCAGGGTAC SEQUENCE_INCLUDED_REGION=0,307 P3_COMMENT=CL is TGAATAACCAATCCACTCCG P3_COMMENT=CR is ACCCTGGAGAAGTAAGCAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.093581 PRIMER_LEFT_0_PENALTY=0.081111 PRIMER_RIGHT_0_PENALTY=0.012470 PRIMER_INTERNAL_0_PENALTY=1.100593 PRIMER_LEFT_0_SEQUENCE=CACAATAAAACCCTGGGGTG PRIMER_RIGHT_0_SEQUENCE=AAGGTCTGCACCTCCTGCTA PRIMER_INTERNAL_0_SEQUENCE=GCCACTCTAGTCCTTGGCTTT PRIMER_LEFT_0=59,20 PRIMER_RIGHT_0=138,20 PRIMER_INTERNAL_0=93,21 PRIMER_LEFT_0_TM=60.081 PRIMER_RIGHT_0_TM=60.012 PRIMER_INTERNAL_0_TM=59.899 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=0.093963 PRIMER_LEFT_1_PENALTY=0.081493 PRIMER_RIGHT_1_PENALTY=0.012470 PRIMER_INTERNAL_1_PENALTY=1.100593 PRIMER_LEFT_1_SEQUENCE=AATAAAACCCTGGGGTGGAC PRIMER_RIGHT_1_SEQUENCE=AAGGTCTGCACCTCCTGCTA PRIMER_INTERNAL_1_SEQUENCE=GCCACTCTAGTCCTTGGCTTT PRIMER_LEFT_1=62,20 PRIMER_RIGHT_1=138,20 PRIMER_INTERNAL_1=93,21 PRIMER_LEFT_1_TM=59.919 PRIMER_RIGHT_1_TM=60.012 PRIMER_INTERNAL_1_TM=59.899 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=0.100814 PRIMER_LEFT_2_PENALTY=0.012470 PRIMER_RIGHT_2_PENALTY=0.088344 PRIMER_INTERNAL_2_PENALTY=0.387849 PRIMER_LEFT_2_SEQUENCE=TAGCAGGAGGTGCAGACCTT PRIMER_RIGHT_2_SEQUENCE=AAGCCTCAGGAGAGGGAGAG PRIMER_INTERNAL_2_SEQUENCE=TCTGGAGGAGTCTCTGGTGG PRIMER_LEFT_2=119,20 PRIMER_RIGHT_2=213,20 PRIMER_INTERNAL_2=143,20 PRIMER_LEFT_2_TM=60.012 PRIMER_RIGHT_2_TM=60.088 PRIMER_INTERNAL_2_TM=60.388 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=95 PRIMER_PAIR_3_PENALTY=0.181120 PRIMER_LEFT_3_PENALTY=0.012470 PRIMER_RIGHT_3_PENALTY=0.168650 PRIMER_INTERNAL_3_PENALTY=0.387849 PRIMER_LEFT_3_SEQUENCE=TAGCAGGAGGTGCAGACCTT PRIMER_RIGHT_3_SEQUENCE=GAGAGGGTGGGGTGAAATTT PRIMER_INTERNAL_3_SEQUENCE=TCTGGAGGAGTCTCTGGTGG PRIMER_LEFT_3=119,20 PRIMER_RIGHT_3=198,20 PRIMER_INTERNAL_3=143,20 PRIMER_LEFT_3_TM=60.012 PRIMER_RIGHT_3_TM=60.169 PRIMER_INTERNAL_3_TM=60.388 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=0.201595 PRIMER_LEFT_4_PENALTY=0.168650 PRIMER_RIGHT_4_PENALTY=0.032945 PRIMER_INTERNAL_4_PENALTY=0.056936 PRIMER_LEFT_4_SEQUENCE=AAATTTCACCCCACCCTCTC PRIMER_RIGHT_4_SEQUENCE=TGAATGCCAAGTGTGGTCAT PRIMER_INTERNAL_4_SEQUENCE=CTTTCTGGGCCTCATCTCTG PRIMER_LEFT_4=179,20 PRIMER_RIGHT_4=264,20 PRIMER_INTERNAL_4=211,20 PRIMER_LEFT_4_TM=60.169 PRIMER_RIGHT_4_TM=59.967 PRIMER_INTERNAL_4_TM=59.943 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.3000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH209 SEQUENCE_TEMPLATE=CTAGCACCATAGGAAGCCACTAATTATATGCGACCAAAGTGATGAACATAGCAAATAGGTGGTACCTGTGGCACATTTTAGGCATTTTAAAATAAATCTTGTGGCAATGCGTTGAAGGTTTGAATGCAAATGGATCAATTCAATATACTTNTATCTCTACAGGATAGTGGCACTTGACAGTTGATAGAAAGGTAGAAAAATTAAAATGATCATCTCTTCCATGGGCCTTCTTCTATCCCTAAGTNAAATTGATTACTTCCTGGCCACCACTGCTTTATCTTTGCGTTTATTATATTTCAGTGACCACATTATATGAT SEQUENCE_INCLUDED_REGION=0,317 P3_COMMENT=CL is CATAGGAAGCCACTAATTATATGCG P3_COMMENT=CR is GTGGTCACTGAAATATAATAAACGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.094290 PRIMER_LEFT_0_PENALTY=0.024663 PRIMER_RIGHT_0_PENALTY=1.069627 PRIMER_INTERNAL_0_PENALTY=0.510724 PRIMER_LEFT_0_SEQUENCE=TAGGTGGTACCTGTGGCACA PRIMER_RIGHT_0_SEQUENCE=TCCATTTGCATTCAAACCTTC PRIMER_INTERNAL_0_SEQUENCE=AAATCTTGTGGCAATGCGTT PRIMER_LEFT_0=55,20 PRIMER_RIGHT_0=133,21 PRIMER_INTERNAL_0=93,20 PRIMER_LEFT_0_TM=60.025 PRIMER_RIGHT_0_TM=59.930 PRIMER_INTERNAL_0_TM=60.511 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=7.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=79 PRIMER_PAIR_1_PENALTY=1.376295 PRIMER_LEFT_1_PENALTY=0.306669 PRIMER_RIGHT_1_PENALTY=1.069627 PRIMER_INTERNAL_1_PENALTY=0.510724 PRIMER_LEFT_1_SEQUENCE=AGGTGGTACCTGTGGCACAT PRIMER_RIGHT_1_SEQUENCE=TCCATTTGCATTCAAACCTTC PRIMER_INTERNAL_1_SEQUENCE=AAATCTTGTGGCAATGCGTT PRIMER_LEFT_1=56,20 PRIMER_RIGHT_1=133,21 PRIMER_INTERNAL_1=93,20 PRIMER_LEFT_1_TM=60.307 PRIMER_RIGHT_1_TM=59.930 PRIMER_INTERNAL_1_TM=60.511 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=38.095 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=1.571022 PRIMER_LEFT_2_PENALTY=1.276733 PRIMER_RIGHT_2_PENALTY=0.294290 PRIMER_INTERNAL_2_PENALTY=0.000037 PRIMER_LEFT_2_SEQUENCE=TGCGACCAAAGTGATGAACA PRIMER_RIGHT_2_SEQUENCE=TTCAAACCTTCAACGCATTG PRIMER_INTERNAL_2_SEQUENCE=ACCTGTGGCACATTTTAGGC PRIMER_LEFT_2=28,20 PRIMER_RIGHT_2=123,20 PRIMER_INTERNAL_2=63,20 PRIMER_LEFT_2_TM=61.277 PRIMER_RIGHT_2_TM=59.706 PRIMER_INTERNAL_2_TM=60.000 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.2000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=1.600009 PRIMER_LEFT_3_PENALTY=1.305719 PRIMER_RIGHT_3_PENALTY=0.294290 PRIMER_INTERNAL_3_PENALTY=5.545167 PRIMER_LEFT_3_SEQUENCE=ATAGGTGGTACCTGTGGCACA PRIMER_RIGHT_3_SEQUENCE=TTGCATTCAAACCTTCAACG PRIMER_INTERNAL_3_SEQUENCE=GCATTTTAAAATAAATCTTGTGGCA PRIMER_LEFT_3=54,21 PRIMER_RIGHT_3=128,20 PRIMER_INTERNAL_3=81,25 PRIMER_LEFT_3_TM=60.306 PRIMER_RIGHT_3_TM=59.706 PRIMER_INTERNAL_3_TM=60.545 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=28.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=10.00 PRIMER_LEFT_3_SELF_END=7.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=8.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=1.621628 PRIMER_LEFT_4_PENALTY=0.036956 PRIMER_RIGHT_4_PENALTY=1.584672 PRIMER_INTERNAL_4_PENALTY=2.431064 PRIMER_LEFT_4_SEQUENCE=ATCTCTTCCATGGGCCTTCT PRIMER_RIGHT_4_SEQUENCE=AACGCAAAGATAAAGCAGTGG PRIMER_INTERNAL_4_SEQUENCE=AAATTGATTACTTCCTGGCCA PRIMER_LEFT_4=211,20 PRIMER_RIGHT_4=286,21 PRIMER_INTERNAL_4=245,21 PRIMER_LEFT_4_TM=60.037 PRIMER_RIGHT_4_TM=59.415 PRIMER_INTERNAL_4_TM=58.569 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH215 SEQUENCE_TEMPLATE=CTCCTGCCCTCCGACGGCGTCTCCAGGCCCCAAACGGCCTCCGGTCGGTGGGCTTCTCCAGGCCCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTTAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG SEQUENCE_INCLUDED_REGION=0,66 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH223 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTTACTACTCTCATATCTATTCANTCCCATCAGTACATAAGAAACTAGGGAAAGAAACATTNTGGTTTGAATCTGTTCTTAAGTAGGNACAATACACATGACTAATTTAAAATATTTTCCAANGTTCTTACACCCGTNATATCTAAATTTGNTACCATCANCTAAAGATTGAAGGCACTGNATTTAAAAAAAAAA SEQUENCE_INCLUDED_REGION=35,199 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH224 SEQUENCE_TEMPLATE=CTAGTTTTGTNATGTATTATTTATTTATTTATTTCTATATTTATTGCAGAGATGAGGTATTACTCTGTTGCCCAGGCTGGTCTCAGACTCCTCAGTGCAAGTNAATCCTCCTCCCTTGGCCTCCCCAAATGCTGGGATTACAGAGGTGAGACATCATGCCTGGCCATGGTTGCTGGTTTTGAAGATGTGGGAAGGGGACCATGAGCCAAGGAGTGCAGGTGGCCTGTAGAATCTGGGAAAGGAAAGGAAGTGGATTCCCCCAGAG SEQUENCE_INCLUDED_REGION=0,265 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.510386 PRIMER_LEFT_0_PENALTY=0.096855 PRIMER_RIGHT_0_PENALTY=0.413531 PRIMER_INTERNAL_0_PENALTY=0.141511 PRIMER_LEFT_0_SEQUENCE=TTTTGAAGATGTGGGAAGGG PRIMER_RIGHT_0_SEQUENCE=TCCACTTCCTTTCCTTTCCC PRIMER_INTERNAL_0_SEQUENCE=GTGCAGGTGGCCTGTAGAAT PRIMER_LEFT_0=176,20 PRIMER_RIGHT_0=253,20 PRIMER_INTERNAL_0=212,20 PRIMER_LEFT_0_TM=59.903 PRIMER_RIGHT_0_TM=60.414 PRIMER_INTERNAL_0_TM=60.142 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=1.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=6.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 PRIMER_PAIR_1_PENALTY=0.722253 PRIMER_LEFT_1_PENALTY=0.308722 PRIMER_RIGHT_1_PENALTY=0.413531 PRIMER_INTERNAL_1_PENALTY=0.141511 PRIMER_LEFT_1_SEQUENCE=TGCTGGTTTTGAAGATGTGG PRIMER_RIGHT_1_SEQUENCE=TCCACTTCCTTTCCTTTCCC PRIMER_INTERNAL_1_SEQUENCE=GTGCAGGTGGCCTGTAGAAT PRIMER_LEFT_1=170,20 PRIMER_RIGHT_1=253,20 PRIMER_INTERNAL_1=212,20 PRIMER_LEFT_1_TM=59.691 PRIMER_RIGHT_1_TM=60.414 PRIMER_INTERNAL_1_TM=60.142 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=84 PRIMER_PAIR_2_PENALTY=0.723698 PRIMER_LEFT_2_PENALTY=0.096855 PRIMER_RIGHT_2_PENALTY=0.626843 PRIMER_INTERNAL_2_PENALTY=0.072951 PRIMER_LEFT_2_SEQUENCE=TTTTGAAGATGTGGGAAGGG PRIMER_RIGHT_2_SEQUENCE=GGGAATCCACTTCCTTTCCT PRIMER_INTERNAL_2_SEQUENCE=GTGGCCTGTAGAATCTGGGA PRIMER_LEFT_2=176,20 PRIMER_RIGHT_2=258,20 PRIMER_INTERNAL_2=218,20 PRIMER_LEFT_2_TM=59.903 PRIMER_RIGHT_2_TM=59.373 PRIMER_INTERNAL_2_TM=60.073 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=0.861343 PRIMER_LEFT_3_PENALTY=0.788392 PRIMER_RIGHT_3_PENALTY=0.072951 PRIMER_INTERNAL_3_PENALTY=0.096855 PRIMER_LEFT_3_SEQUENCE=GAGGTGAGACATCATGCCTG PRIMER_RIGHT_3_SEQUENCE=TCCCAGATTCTACAGGCCAC PRIMER_INTERNAL_3_SEQUENCE=TTTTGAAGATGTGGGAAGGG PRIMER_LEFT_3=142,20 PRIMER_RIGHT_3=237,20 PRIMER_INTERNAL_3=176,20 PRIMER_LEFT_3_TM=59.212 PRIMER_RIGHT_3_TM=60.073 PRIMER_INTERNAL_3_TM=59.903 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=96 PRIMER_PAIR_4_PENALTY=0.861343 PRIMER_LEFT_4_PENALTY=0.788392 PRIMER_RIGHT_4_PENALTY=0.072951 PRIMER_INTERNAL_4_PENALTY=0.096855 PRIMER_LEFT_4_SEQUENCE=CAGAGGTGAGACATCATGCC PRIMER_RIGHT_4_SEQUENCE=TCCCAGATTCTACAGGCCAC PRIMER_INTERNAL_4_SEQUENCE=TTTTGAAGATGTGGGAAGGG PRIMER_LEFT_4=140,20 PRIMER_RIGHT_4=237,20 PRIMER_INTERNAL_4=176,20 PRIMER_LEFT_4_TM=59.212 PRIMER_RIGHT_4_TM=60.073 PRIMER_INTERNAL_4_TM=59.903 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.6000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH227 SEQUENCE_TEMPLATE=CTTTTGGTNNTTAAGCCCTGGGTNCTTTCACCTCCACAAAGATGCTTCACAGAGGTGTAAAGTGTNTTAGTCTCAATGGTCTTATACGGCGTTCAATCACATGAGACTCAAGAGGAGCAGTCACCTCAGTAATCTTAATTTTCTCTGTGCTTTCCTTCTGCTAGAAATCTTGGCCCTCCATCATAGAATTATCTGGAAAAGCCCAGGGGAGTTGAAATATGACTCAGTGCAATTCTATTTTTAGACACAGACTTAATGGTTGACTCCCATTGAAATAACTGAAATACAG SEQUENCE_INCLUDED_REGION=0,289 P3_COMMENT=CL is TCAATGGTCTTATACGGCGT P3_COMMENT=CR is TTCAATGGGAGTCAACCATT P3_COMMENT=CM is CAATCACATGAGACTCAAGAGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.748631 PRIMER_LEFT_0_PENALTY=0.575240 PRIMER_RIGHT_0_PENALTY=0.173391 PRIMER_INTERNAL_0_PENALTY=0.875462 PRIMER_LEFT_0_SEQUENCE=ATACGGCGTTCAATCACATG PRIMER_RIGHT_0_SEQUENCE=GGAGGGCCAAGATTTCTAGC PRIMER_INTERNAL_0_SEQUENCE=CAAGAGGAGCAGTCACCTCA PRIMER_LEFT_0=83,20 PRIMER_RIGHT_0=178,20 PRIMER_INTERNAL_0=108,20 PRIMER_LEFT_0_TM=59.425 PRIMER_RIGHT_0_TM=60.173 PRIMER_INTERNAL_0_TM=59.125 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=96 PRIMER_PAIR_1_PENALTY=0.904425 PRIMER_LEFT_1_PENALTY=0.875462 PRIMER_RIGHT_1_PENALTY=0.028963 PRIMER_INTERNAL_1_PENALTY=1.251196 PRIMER_LEFT_1_SEQUENCE=CAAGAGGAGCAGTCACCTCA PRIMER_RIGHT_1_SEQUENCE=TTCTATGATGGAGGGCCAAG PRIMER_INTERNAL_1_SEQUENCE=TTCTCTGTGCTTTCCTTCTGC PRIMER_LEFT_1=108,20 PRIMER_RIGHT_1=187,20 PRIMER_INTERNAL_1=140,21 PRIMER_LEFT_1_TM=59.125 PRIMER_RIGHT_1_TM=60.029 PRIMER_INTERNAL_1_TM=59.749 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=0.984751 PRIMER_LEFT_2_PENALTY=0.575240 PRIMER_RIGHT_2_PENALTY=0.409511 PRIMER_INTERNAL_2_PENALTY=0.875462 PRIMER_LEFT_2_SEQUENCE=ATACGGCGTTCAATCACATG PRIMER_RIGHT_2_SEQUENCE=GATGGAGGGCCAAGATTTCT PRIMER_INTERNAL_2_SEQUENCE=CAAGAGGAGCAGTCACCTCA PRIMER_LEFT_2=83,20 PRIMER_RIGHT_2=181,20 PRIMER_INTERNAL_2=108,20 PRIMER_LEFT_2_TM=59.425 PRIMER_RIGHT_2_TM=60.410 PRIMER_INTERNAL_2_TM=59.125 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=1.079907 PRIMER_LEFT_3_PENALTY=0.906516 PRIMER_RIGHT_3_PENALTY=0.173391 PRIMER_INTERNAL_3_PENALTY=0.875462 PRIMER_LEFT_3_SEQUENCE=GGCGTTCAATCACATGAGAC PRIMER_RIGHT_3_SEQUENCE=GGAGGGCCAAGATTTCTAGC PRIMER_INTERNAL_3_SEQUENCE=CAAGAGGAGCAGTCACCTCA PRIMER_LEFT_3=87,20 PRIMER_RIGHT_3=178,20 PRIMER_INTERNAL_3=108,20 PRIMER_LEFT_3_TM=59.093 PRIMER_RIGHT_3_TM=60.173 PRIMER_INTERNAL_3_TM=59.125 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.1000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=92 PRIMER_PAIR_4_PENALTY=1.164835 PRIMER_LEFT_4_PENALTY=0.575240 PRIMER_RIGHT_4_PENALTY=0.589594 PRIMER_INTERNAL_4_PENALTY=0.875462 PRIMER_LEFT_4_SEQUENCE=ATACGGCGTTCAATCACATG PRIMER_RIGHT_4_SEQUENCE=GGGCCAAGATTTCTAGCAGA PRIMER_INTERNAL_4_SEQUENCE=CAAGAGGAGCAGTCACCTCA PRIMER_LEFT_4=83,20 PRIMER_RIGHT_4=175,20 PRIMER_INTERNAL_4=108,20 PRIMER_LEFT_4_TM=59.425 PRIMER_RIGHT_4_TM=59.410 PRIMER_INTERNAL_4_TM=59.125 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=93 = SEQUENCE_ID=MH229 SEQUENCE_TEMPLATE=CTAAATATTCCAGGTCAGTGAAAATGCAAAGACATTTTAGGTCACAGGAAACCACCTGGGATGTTTCAGCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCA SEQUENCE_INCLUDED_REGION=0,72 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH232 SEQUENCE_TEMPLATE=CTACTAAGCAAATTCTGCTCAGCCTTCTGCCTTTCCTGACTGTCTTGTTGGCCCTTCCCACTTTAAGGATGCCTGTTTAAGTAGCCACCTCTAATTAGGAATCTTCCCTTGTTCTTTCTCAGGAGGCTTAGACACTGTCAGTTTCCTGAAGACAGAAAATAAGCCTGCATTATCCTAGTAGTGGATTCAAAACTAATTGTGTCCTGAGTCTTTCAATCATCAAGACATGGGAAACACTCAACAG SEQUENCE_INCLUDED_REGION=0,244 P3_COMMENT=CL is TTCTGCCTTTCCTGACTGTC P3_COMMENT=CR is TGTTTCCCATGTCTTGATGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.521276 PRIMER_LEFT_0_PENALTY=0.296722 PRIMER_RIGHT_0_PENALTY=1.224554 PRIMER_INTERNAL_0_PENALTY=1.761718 PRIMER_LEFT_0_SEQUENCE=GACTGTCTTGTTGGCCCTTC PRIMER_RIGHT_0_SEQUENCE=CCTCCTGAGAAAGAACAAGGG PRIMER_INTERNAL_0_SEQUENCE=GGATGCCTGTTTAAGTAGCCA PRIMER_LEFT_0=37,20 PRIMER_RIGHT_0=125,21 PRIMER_INTERNAL_0=66,21 PRIMER_LEFT_0_TM=59.703 PRIMER_RIGHT_0_TM=60.225 PRIMER_INTERNAL_0_TM=59.238 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_INTERNAL_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=89 PRIMER_PAIR_1_PENALTY=1.522572 PRIMER_LEFT_1_PENALTY=0.298018 PRIMER_RIGHT_1_PENALTY=1.224554 PRIMER_INTERNAL_1_PENALTY=0.066323 PRIMER_LEFT_1_SEQUENCE=CTGACTGTCTTGTTGGCCCT PRIMER_RIGHT_1_SEQUENCE=CCTCCTGAGAAAGAACAAGGG PRIMER_INTERNAL_1_SEQUENCE=TCCCACTTTAAGGATGCCTG PRIMER_LEFT_1=35,20 PRIMER_RIGHT_1=125,21 PRIMER_INTERNAL_1=55,20 PRIMER_LEFT_1_TM=60.298 PRIMER_RIGHT_1_TM=60.225 PRIMER_INTERNAL_1_TM=60.066 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=52.381 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=10.9000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=1.664438 PRIMER_LEFT_2_PENALTY=0.296722 PRIMER_RIGHT_2_PENALTY=1.367716 PRIMER_INTERNAL_2_PENALTY=1.761718 PRIMER_LEFT_2_SEQUENCE=GACTGTCTTGTTGGCCCTTC PRIMER_RIGHT_2_SEQUENCE=GCCTCCTGAGAAAGAACAAGG PRIMER_INTERNAL_2_SEQUENCE=GGATGCCTGTTTAAGTAGCCA PRIMER_LEFT_2=37,20 PRIMER_RIGHT_2=126,21 PRIMER_INTERNAL_2=66,21 PRIMER_LEFT_2_TM=59.703 PRIMER_RIGHT_2_TM=60.368 PRIMER_INTERNAL_2_TM=59.238 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_INTERNAL_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=1.665734 PRIMER_LEFT_3_PENALTY=0.298018 PRIMER_RIGHT_3_PENALTY=1.367716 PRIMER_INTERNAL_3_PENALTY=0.066323 PRIMER_LEFT_3_SEQUENCE=CTGACTGTCTTGTTGGCCCT PRIMER_RIGHT_3_SEQUENCE=GCCTCCTGAGAAAGAACAAGG PRIMER_INTERNAL_3_SEQUENCE=TCCCACTTTAAGGATGCCTG PRIMER_LEFT_3=35,20 PRIMER_RIGHT_3=126,21 PRIMER_INTERNAL_3=55,20 PRIMER_LEFT_3_TM=60.298 PRIMER_RIGHT_3_TM=60.368 PRIMER_INTERNAL_3_TM=60.066 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=92 PRIMER_PAIR_4_PENALTY=1.804742 PRIMER_LEFT_4_PENALTY=0.580189 PRIMER_RIGHT_4_PENALTY=1.224554 PRIMER_INTERNAL_4_PENALTY=2.978554 PRIMER_LEFT_4_SEQUENCE=GCCCTTCCCACTTTAAGGAT PRIMER_RIGHT_4_SEQUENCE=CCTCCTGAGAAAGAACAAGGG PRIMER_INTERNAL_4_SEQUENCE=GCCTGTTTAAGTAGCCACCTC PRIMER_LEFT_4=50,20 PRIMER_RIGHT_4=125,21 PRIMER_INTERNAL_4=70,21 PRIMER_LEFT_4_TM=59.420 PRIMER_RIGHT_4_TM=60.225 PRIMER_INTERNAL_4_TM=58.021 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.8000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH233 SEQUENCE_TEMPLATE=CTTATTTAAATAAATAATGTCTGAAAACTTCCCAAATCTTGGTAGGAATATGGCTATCCATAATCATGAGGCTCAAGCAACATTAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGNGAAATNGTTATCCGCTNACANTTCCACANAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTANTGAGTNAGCTAACTCNCATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCA SEQUENCE_INCLUDED_REGION=0,86 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=7.722379 PRIMER_LEFT_0_PENALTY=5.626841 PRIMER_RIGHT_0_PENALTY=2.095538 PRIMER_INTERNAL_0_PENALTY=3.527447 PRIMER_LEFT_0_SEQUENCE=AAATAATGTCTGAAAACTTCCCAAA PRIMER_RIGHT_0_SEQUENCE=CTAATGTTGCTTGAGCCTCATG PRIMER_INTERNAL_0_SEQUENCE=TTGGTAGGAATATGGCTATCCA PRIMER_LEFT_0=11,25 PRIMER_RIGHT_0=85,22 PRIMER_INTERNAL_0=38,22 PRIMER_LEFT_0_TM=59.373 PRIMER_RIGHT_0_TM=59.904 PRIMER_INTERNAL_0_TM=58.473 PRIMER_LEFT_0_GC_PERCENT=28.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=7.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=7.895837 PRIMER_LEFT_1_PENALTY=5.800299 PRIMER_RIGHT_1_PENALTY=2.095538 PRIMER_INTERNAL_1_PENALTY=3.527447 PRIMER_LEFT_1_SEQUENCE=AAATAATGTCTGAAAACTTCCCAA PRIMER_RIGHT_1_SEQUENCE=CTAATGTTGCTTGAGCCTCATG PRIMER_INTERNAL_1_SEQUENCE=TTGGTAGGAATATGGCTATCCA PRIMER_LEFT_1=11,24 PRIMER_RIGHT_1=85,22 PRIMER_INTERNAL_1=38,22 PRIMER_LEFT_1_TM=58.200 PRIMER_RIGHT_1_TM=59.904 PRIMER_INTERNAL_1_TM=58.473 PRIMER_LEFT_1_GC_PERCENT=29.167 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_INTERNAL_1_SELF_END=8.00 PRIMER_LEFT_1_END_STABILITY=10.0000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=8.735608 PRIMER_LEFT_2_PENALTY=7.612130 PRIMER_RIGHT_2_PENALTY=1.123478 PRIMER_INTERNAL_2_PENALTY=3.527447 PRIMER_LEFT_2_SEQUENCE=ATAAATAATGTCTGAAAACTTCCCA PRIMER_RIGHT_2_SEQUENCE=AATGTTGCTTGAGCCTCATG PRIMER_INTERNAL_2_SEQUENCE=TTGGTAGGAATATGGCTATCCA PRIMER_LEFT_2=9,25 PRIMER_RIGHT_2=83,20 PRIMER_INTERNAL_2=38,22 PRIMER_LEFT_2_TM=57.388 PRIMER_RIGHT_2_TM=58.877 PRIMER_INTERNAL_2_TM=58.473 PRIMER_LEFT_2_GC_PERCENT=28.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=7.00 PRIMER_INTERNAL_2_SELF_END=8.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=8.802618 PRIMER_LEFT_3_PENALTY=6.733780 PRIMER_RIGHT_3_PENALTY=2.068838 PRIMER_INTERNAL_3_PENALTY=3.527447 PRIMER_LEFT_3_SEQUENCE=TAAATAATGTCTGAAAACTTCCCAA PRIMER_RIGHT_3_SEQUENCE=TAATGTTGCTTGAGCCTCATG PRIMER_INTERNAL_3_SEQUENCE=TTGGTAGGAATATGGCTATCCA PRIMER_LEFT_3=10,25 PRIMER_RIGHT_3=84,21 PRIMER_INTERNAL_3=38,22 PRIMER_LEFT_3_TM=58.266 PRIMER_RIGHT_3_TM=58.931 PRIMER_INTERNAL_3_TM=58.473 PRIMER_LEFT_3_GC_PERCENT=28.000 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=7.00 PRIMER_INTERNAL_3_SELF_END=8.00 PRIMER_LEFT_3_END_STABILITY=10.0000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=8.829318 PRIMER_LEFT_4_PENALTY=6.733780 PRIMER_RIGHT_4_PENALTY=2.095538 PRIMER_INTERNAL_4_PENALTY=3.527447 PRIMER_LEFT_4_SEQUENCE=TAAATAATGTCTGAAAACTTCCCAA PRIMER_RIGHT_4_SEQUENCE=CTAATGTTGCTTGAGCCTCATG PRIMER_INTERNAL_4_SEQUENCE=TTGGTAGGAATATGGCTATCCA PRIMER_LEFT_4=10,25 PRIMER_RIGHT_4=85,22 PRIMER_INTERNAL_4=38,22 PRIMER_LEFT_4_TM=58.266 PRIMER_RIGHT_4_TM=59.904 PRIMER_INTERNAL_4_TM=58.473 PRIMER_LEFT_4_GC_PERCENT=28.000 PRIMER_RIGHT_4_GC_PERCENT=45.455 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=7.00 PRIMER_INTERNAL_4_SELF_END=8.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH238 SEQUENCE_TEMPLATE=AAAGACTACCCATAGTATGAGTCAATTTATTTTAAAATTACAGAAGATGCTAAACTAGGAGCAGCAAGGAGATTAGTACTTTCAGAGATCAGGGCAAGGGAAGGGGCTTGACTGCAAATGGACACAAGAAAACTTTTTAAGTAACGAATATGTTCTGTATCATGATTCTGATCATGGTTTAANTACCTGTCAAAATTCATCAATTATACATTTTAAATTGGTAAACTTTATTCCAAGTAATTCATACCT SEQUENCE_INCLUDED_REGION=0,249 P3_COMMENT=CL is AAGATGCTAAACTAGGAGCAGC P3_COMMENT=CR is GAATTACTTGGAATAAAGTTTACCA P3_COMMENT=CM is GATTAGTACTTTCAGAGATCAGGGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.871921 PRIMER_LEFT_0_PENALTY=2.169174 PRIMER_RIGHT_0_PENALTY=0.702747 PRIMER_INTERNAL_0_PENALTY=0.331043 PRIMER_LEFT_0_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA PRIMER_RIGHT_0_SEQUENCE=GTGTCCATTTGCAGTCAAGC PRIMER_INTERNAL_0_SEQUENCE=TTTCAGAGATCAGGGCAAGG PRIMER_LEFT_0=48,22 PRIMER_RIGHT_0=124,20 PRIMER_INTERNAL_0=79,20 PRIMER_LEFT_0_TM=60.169 PRIMER_RIGHT_0_TM=59.297 PRIMER_INTERNAL_0_TM=60.331 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=3.263418 PRIMER_LEFT_1_PENALTY=2.560670 PRIMER_RIGHT_1_PENALTY=0.702747 PRIMER_INTERNAL_1_PENALTY=0.331043 PRIMER_LEFT_1_SEQUENCE=GCTAAACTAGGAGCAGCAAGG PRIMER_RIGHT_1_SEQUENCE=GTGTCCATTTGCAGTCAAGC PRIMER_INTERNAL_1_SEQUENCE=TTTCAGAGATCAGGGCAAGG PRIMER_LEFT_1=48,21 PRIMER_RIGHT_1=124,20 PRIMER_INTERNAL_1=79,20 PRIMER_LEFT_1_TM=58.439 PRIMER_RIGHT_1_TM=59.297 PRIMER_INTERNAL_1_TM=60.331 PRIMER_LEFT_1_GC_PERCENT=52.381 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=3.420435 PRIMER_LEFT_2_PENALTY=2.169174 PRIMER_RIGHT_2_PENALTY=1.251261 PRIMER_INTERNAL_2_PENALTY=0.331043 PRIMER_LEFT_2_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA PRIMER_RIGHT_2_SEQUENCE=TTGTGTCCATTTGCAGTCAAG PRIMER_INTERNAL_2_SEQUENCE=TTTCAGAGATCAGGGCAAGG PRIMER_LEFT_2=48,22 PRIMER_RIGHT_2=126,21 PRIMER_INTERNAL_2=79,20 PRIMER_LEFT_2_TM=60.169 PRIMER_RIGHT_2_TM=59.749 PRIMER_INTERNAL_2_TM=60.331 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=79 PRIMER_PAIR_3_PENALTY=3.420435 PRIMER_LEFT_3_PENALTY=2.169174 PRIMER_RIGHT_3_PENALTY=1.251261 PRIMER_INTERNAL_3_PENALTY=0.331043 PRIMER_LEFT_3_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA PRIMER_RIGHT_3_SEQUENCE=CTTGTGTCCATTTGCAGTCAA PRIMER_INTERNAL_3_SEQUENCE=TTTCAGAGATCAGGGCAAGG PRIMER_LEFT_3=48,22 PRIMER_RIGHT_3=127,21 PRIMER_INTERNAL_3=79,20 PRIMER_LEFT_3_TM=60.169 PRIMER_RIGHT_3_TM=59.749 PRIMER_INTERNAL_3_TM=60.331 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=3.463126 PRIMER_LEFT_4_PENALTY=2.169174 PRIMER_RIGHT_4_PENALTY=1.293952 PRIMER_INTERNAL_4_PENALTY=0.331043 PRIMER_LEFT_4_SEQUENCE=GCTAAACTAGGAGCAGCAAGGA PRIMER_RIGHT_4_SEQUENCE=TCTTGTGTCCATTTGCAGTCA PRIMER_INTERNAL_4_SEQUENCE=TTTCAGAGATCAGGGCAAGG PRIMER_LEFT_4=48,22 PRIMER_RIGHT_4=128,21 PRIMER_INTERNAL_4=79,20 PRIMER_LEFT_4_TM=60.169 PRIMER_RIGHT_4_TM=60.294 PRIMER_INTERNAL_4_TM=60.331 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=81 = SEQUENCE_ID=MH240 SEQUENCE_TEMPLATE=CAGATCTGTAATCTGCTTTCTAGATCCCTTTCTTACCCATTCTTAAGCCATCACAGCAGAGTACCTTTCTAACTTATAAGATTGTGTAGAGGTTGGAAGGAGGACAGGACTGTTCTGTTGGTATAATGACCCTGTGTCCAGTTAATCCATG SEQUENCE_INCLUDED_REGION=0,151 P3_COMMENT=CL is AGCCATCACAGCAGAGTACC P3_COMMENT=CR is TGGATTAACTGGACACAGGG P3_COMMENT=CM is GTGTAGAGGTTGGAAGGAGGA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.067153 PRIMER_LEFT_0_PENALTY=1.278288 PRIMER_RIGHT_0_PENALTY=1.788865 PRIMER_INTERNAL_0_PENALTY=4.295192 PRIMER_LEFT_0_SEQUENCE=CCATTCTTAAGCCATCACAGC PRIMER_RIGHT_0_SEQUENCE=CCAACAGAACAGTCCTGTCCT PRIMER_INTERNAL_0_SEQUENCE=AAGATTGTGTAGAGGTTGGAAGG PRIMER_LEFT_0=36,21 PRIMER_RIGHT_0=120,21 PRIMER_INTERNAL_0=77,23 PRIMER_LEFT_0_TM=59.722 PRIMER_RIGHT_0_TM=59.211 PRIMER_INTERNAL_0_TM=58.705 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_INTERNAL_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=9.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=8.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=85 PRIMER_PAIR_1_PENALTY=3.165558 PRIMER_LEFT_1_PENALTY=1.278288 PRIMER_RIGHT_1_PENALTY=1.887270 PRIMER_INTERNAL_1_PENALTY=3.825817 PRIMER_LEFT_1_SEQUENCE=CCATTCTTAAGCCATCACAGC PRIMER_RIGHT_1_SEQUENCE=CCAACAGAACAGTCCTGTCC PRIMER_INTERNAL_1_SEQUENCE=GATTGTGTAGAGGTTGGAAGGA PRIMER_LEFT_1=36,21 PRIMER_RIGHT_1=120,20 PRIMER_INTERNAL_1=79,22 PRIMER_LEFT_1_TM=59.722 PRIMER_RIGHT_1_TM=58.113 PRIMER_INTERNAL_1_TM=58.174 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=9.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=85 PRIMER_PAIR_2_PENALTY=3.211098 PRIMER_LEFT_2_PENALTY=1.422232 PRIMER_RIGHT_2_PENALTY=1.788865 PRIMER_INTERNAL_2_PENALTY=4.295192 PRIMER_LEFT_2_SEQUENCE=CCCATTCTTAAGCCATCACAG PRIMER_RIGHT_2_SEQUENCE=CCAACAGAACAGTCCTGTCCT PRIMER_INTERNAL_2_SEQUENCE=AAGATTGTGTAGAGGTTGGAAGG PRIMER_LEFT_2=35,21 PRIMER_RIGHT_2=120,21 PRIMER_INTERNAL_2=77,23 PRIMER_LEFT_2_TM=59.578 PRIMER_RIGHT_2_TM=59.211 PRIMER_INTERNAL_2_TM=58.705 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_INTERNAL_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=9.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=8.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=86 PRIMER_PAIR_3_PENALTY=3.214224 PRIMER_LEFT_3_PENALTY=1.278288 PRIMER_RIGHT_3_PENALTY=1.935937 PRIMER_INTERNAL_3_PENALTY=3.825817 PRIMER_LEFT_3_SEQUENCE=CCATTCTTAAGCCATCACAGC PRIMER_RIGHT_3_SEQUENCE=ACCAACAGAACAGTCCTGTCC PRIMER_INTERNAL_3_SEQUENCE=GATTGTGTAGAGGTTGGAAGGA PRIMER_LEFT_3=36,21 PRIMER_RIGHT_3=121,21 PRIMER_INTERNAL_3=79,22 PRIMER_LEFT_3_TM=59.722 PRIMER_RIGHT_3_TM=59.064 PRIMER_INTERNAL_3_TM=58.174 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_INTERNAL_3_GC_PERCENT=45.455 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=9.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=86 PRIMER_PAIR_4_PENALTY=3.216673 PRIMER_LEFT_4_PENALTY=1.427808 PRIMER_RIGHT_4_PENALTY=1.788865 PRIMER_INTERNAL_4_PENALTY=4.295192 PRIMER_LEFT_4_SEQUENCE=CCCATTCTTAAGCCATCACA PRIMER_RIGHT_4_SEQUENCE=CCAACAGAACAGTCCTGTCCT PRIMER_INTERNAL_4_SEQUENCE=AAGATTGTGTAGAGGTTGGAAGG PRIMER_LEFT_4=35,20 PRIMER_RIGHT_4=120,21 PRIMER_INTERNAL_4=77,23 PRIMER_LEFT_4_TM=58.572 PRIMER_RIGHT_4_TM=59.211 PRIMER_INTERNAL_4_TM=58.705 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=9.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=8.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH243 SEQUENCE_TEMPLATE=NGGNNNCCNNCAGNGNCTGAAGGAAAGNACTGGCTTTTGGGGTACAAGGGTGACTAGAACCTTNCTGGGACTNTGGCTATAGTACTTTGAAATTATCACCCATATAAAAGAGGGACATGGACTATAACGTTGATTTCTTATTGCTGTACATTGCGTTTTTAACCTGCAAGTTCTCAGTTTCTTCACCATNACTCCATCAAAGNCCCTGGCTATAAGATCTGGATTTTACCCACTCCATCTTCTCTTTCCTTCTTACTGTGTNAATTCCTATTTTCTTTCAGAATCTTCTAAAAACAGTTGTATCTAACCGCATGTGCTGTTGTG SEQUENCE_INCLUDED_REGION=0,324 P3_COMMENT=CL is TTGGGGTACAAGGGTGACTA P3_COMMENT=CR is TGGAGTGGGTAAAATCCAGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.271416 PRIMER_LEFT_0_PENALTY=1.059316 PRIMER_RIGHT_0_PENALTY=0.212099 PRIMER_INTERNAL_0_PENALTY=8.275230 PRIMER_LEFT_0_SEQUENCE=CCCACTCCATCTTCTCTTTCC PRIMER_RIGHT_0_SEQUENCE=ACAACAGCACATGCGGTTAG PRIMER_INTERNAL_0_SEQUENCE=TCTTTCAGAATCTTCTAAAAACAGTTG PRIMER_LEFT_0=228,21 PRIMER_RIGHT_0=322,20 PRIMER_INTERNAL_0=273,27 PRIMER_LEFT_0_TM=60.059 PRIMER_RIGHT_0_TM=59.788 PRIMER_INTERNAL_0_TM=58.725 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=29.630 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=5.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=95 PRIMER_PAIR_1_PENALTY=1.924735 PRIMER_LEFT_1_PENALTY=1.059316 PRIMER_RIGHT_1_PENALTY=0.865419 PRIMER_INTERNAL_1_PENALTY=8.275230 PRIMER_LEFT_1_SEQUENCE=CCCACTCCATCTTCTCTTTCC PRIMER_RIGHT_1_SEQUENCE=CAACAGCACATGCGGTTAGA PRIMER_INTERNAL_1_SEQUENCE=TCTTTCAGAATCTTCTAAAAACAGTTG PRIMER_LEFT_1=228,21 PRIMER_RIGHT_1=321,20 PRIMER_INTERNAL_1=273,27 PRIMER_LEFT_1_TM=60.059 PRIMER_RIGHT_1_TM=60.865 PRIMER_INTERNAL_1_TM=58.725 PRIMER_LEFT_1_GC_PERCENT=52.381 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=29.630 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=6.0000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=94 PRIMER_PAIR_2_PENALTY=2.056572 PRIMER_LEFT_2_PENALTY=0.997256 PRIMER_RIGHT_2_PENALTY=1.059316 PRIMER_INTERNAL_2_PENALTY=3.152909 PRIMER_LEFT_2_SEQUENCE=TTGCGTTTTTAACCTGCAAG PRIMER_RIGHT_2_SEQUENCE=GGAAAGAGAAGATGGAGTGGG PRIMER_INTERNAL_2_SEQUENCE=CCCTGGCTATAAGATCTGGATTT PRIMER_LEFT_2=150,20 PRIMER_RIGHT_2=248,21 PRIMER_INTERNAL_2=203,23 PRIMER_LEFT_2_TM=59.003 PRIMER_RIGHT_2_TM=60.059 PRIMER_INTERNAL_2_TM=59.847 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_INTERNAL_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=2.250975 PRIMER_LEFT_3_PENALTY=1.191659 PRIMER_RIGHT_3_PENALTY=1.059316 PRIMER_INTERNAL_3_PENALTY=3.152909 PRIMER_LEFT_3_SEQUENCE=TGCGTTTTTAACCTGCAAGTT PRIMER_RIGHT_3_SEQUENCE=GGAAAGAGAAGATGGAGTGGG PRIMER_INTERNAL_3_SEQUENCE=CCCTGGCTATAAGATCTGGATTT PRIMER_LEFT_3=151,21 PRIMER_RIGHT_3=248,21 PRIMER_INTERNAL_3=203,23 PRIMER_LEFT_3_TM=59.808 PRIMER_RIGHT_3_TM=60.059 PRIMER_INTERNAL_3_TM=59.847 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_INTERNAL_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=8.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=2.250975 PRIMER_LEFT_4_PENALTY=1.191659 PRIMER_RIGHT_4_PENALTY=1.059316 PRIMER_INTERNAL_4_PENALTY=3.152909 PRIMER_LEFT_4_SEQUENCE=TTGCGTTTTTAACCTGCAAGT PRIMER_RIGHT_4_SEQUENCE=GGAAAGAGAAGATGGAGTGGG PRIMER_INTERNAL_4_SEQUENCE=CCCTGGCTATAAGATCTGGATTT PRIMER_LEFT_4=150,21 PRIMER_RIGHT_4=248,21 PRIMER_INTERNAL_4=203,23 PRIMER_LEFT_4_TM=59.808 PRIMER_RIGHT_4_TM=60.059 PRIMER_INTERNAL_4_TM=59.847 PRIMER_LEFT_4_GC_PERCENT=38.095 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = SEQUENCE_ID=MH245 SEQUENCE_TEMPLATE=AAGCACAGTGGATATTTTTAGGCCTAGCATTCTTTATATTTATCCACTTAAAAATATATTAGTTTCACCTAGATCTAGGTAAAATAGTTTGCAAAAAGATTTTAGAATTGGGCAATTAATTTTAATTCTGACATCTTGGGCTTTTCACTTTTNCTTAGGCTCTCCTCGCTGACTTCGTTATTTACTTCATCTAGCATCCATCTTAACTTCTGTGTGAGCCCCTTGCCTTTGAAAACATGTCTTACTTTGT SEQUENCE_INCLUDED_REGION=0,250 P3_COMMENT=CL is AGCACAGTGGATATTTTTAGGC P3_COMMENT=CR is GGGGCTCACACAGAAGTTAA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.727342 PRIMER_LEFT_0_PENALTY=0.254998 PRIMER_RIGHT_0_PENALTY=0.472344 PRIMER_INTERNAL_0_PENALTY=4.219443 PRIMER_LEFT_0_SEQUENCE=CTCTCCTCGCTGACTTCGTT PRIMER_RIGHT_0_SEQUENCE=CATGTTTTCAAAGGCAAGGG PRIMER_INTERNAL_0_SEQUENCE=GCATCCATCTTAACTTCTGTGTG PRIMER_LEFT_0=159,20 PRIMER_RIGHT_0=238,20 PRIMER_INTERNAL_0=193,23 PRIMER_LEFT_0_TM=59.745 PRIMER_RIGHT_0_TM=60.472 PRIMER_INTERNAL_0_TM=58.781 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=1.139469 PRIMER_LEFT_1_PENALTY=0.667126 PRIMER_RIGHT_1_PENALTY=0.472344 PRIMER_INTERNAL_1_PENALTY=4.219443 PRIMER_LEFT_1_SEQUENCE=TAGGCTCTCCTCGCTGACTT PRIMER_RIGHT_1_SEQUENCE=CATGTTTTCAAAGGCAAGGG PRIMER_INTERNAL_1_SEQUENCE=GCATCCATCTTAACTTCTGTGTG PRIMER_LEFT_1=155,20 PRIMER_RIGHT_1=238,20 PRIMER_INTERNAL_1=193,23 PRIMER_LEFT_1_TM=59.333 PRIMER_RIGHT_1_TM=60.472 PRIMER_INTERNAL_1_TM=58.781 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=43.478 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=84 PRIMER_PAIR_2_PENALTY=1.139469 PRIMER_LEFT_2_PENALTY=0.667126 PRIMER_RIGHT_2_PENALTY=0.472344 PRIMER_INTERNAL_2_PENALTY=4.219443 PRIMER_LEFT_2_SEQUENCE=TTAGGCTCTCCTCGCTGACT PRIMER_RIGHT_2_SEQUENCE=CATGTTTTCAAAGGCAAGGG PRIMER_INTERNAL_2_SEQUENCE=GCATCCATCTTAACTTCTGTGTG PRIMER_LEFT_2=154,20 PRIMER_RIGHT_2=238,20 PRIMER_INTERNAL_2=193,23 PRIMER_LEFT_2_TM=59.333 PRIMER_RIGHT_2_TM=60.472 PRIMER_INTERNAL_2_TM=58.781 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=85 PRIMER_PAIR_3_PENALTY=1.139469 PRIMER_LEFT_3_PENALTY=0.667126 PRIMER_RIGHT_3_PENALTY=0.472344 PRIMER_INTERNAL_3_PENALTY=4.219443 PRIMER_LEFT_3_SEQUENCE=CTTAGGCTCTCCTCGCTGAC PRIMER_RIGHT_3_SEQUENCE=CATGTTTTCAAAGGCAAGGG PRIMER_INTERNAL_3_SEQUENCE=GCATCCATCTTAACTTCTGTGTG PRIMER_LEFT_3=153,20 PRIMER_RIGHT_3=238,20 PRIMER_INTERNAL_3=193,23 PRIMER_LEFT_3_TM=59.333 PRIMER_RIGHT_3_TM=60.472 PRIMER_INTERNAL_3_TM=58.781 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=86 PRIMER_PAIR_4_PENALTY=1.450582 PRIMER_LEFT_4_PENALTY=1.152564 PRIMER_RIGHT_4_PENALTY=0.298018 PRIMER_INTERNAL_4_PENALTY=0.254998 PRIMER_LEFT_4_SEQUENCE=CTGACATCTTGGGCTTTTCA PRIMER_RIGHT_4_SEQUENCE=CAAGGGGCTCACACAGAAGT PRIMER_INTERNAL_4_SEQUENCE=CTCTCCTCGCTGACTTCGTT PRIMER_LEFT_4=127,20 PRIMER_RIGHT_4=224,20 PRIMER_INTERNAL_4=159,20 PRIMER_LEFT_4_TM=58.847 PRIMER_RIGHT_4_TM=60.298 PRIMER_INTERNAL_4_TM=59.745 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH250 SEQUENCE_TEMPLATE=TGAATCTAGTTCAGATAAAAAATGTGCTCAACCTCAGATAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=0,41 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH268 SEQUENCE_TEMPLATE=GCTCGCATCCCTCCAGGTCGACTCTAGAGGATCCCCCTTCATTTAATCAAAAGTTTAAAATTATAAGCATTAAAATGGCTGATAAATCACCTGCTAAAACCCTAGGAAAAAAATCTGCAACATGGAACAGGGACAGAAATCGTTAACTAAAGAGAAGAACTGGAAATTAAAAATGAGCATGAAATATGGTAGGATTAATAAANCAATAATAAGATAATCAGCAAGTTGGAAGTGAAATGGGATAATGTAGAAATTATGTTAGGACAGAAATTTACATCCTTATAACAG SEQUENCE_INCLUDED_REGION=35,253 P3_COMMENT=CL is TGGCTGATAAATCACCTGCT P3_COMMENT=CR is AGGATGTAAATTTCTGTCCTAACA P3_COMMENT=CM is AAACCCTAGGAAAAAAATCTGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.532615 PRIMER_LEFT_0_PENALTY=0.480482 PRIMER_RIGHT_0_PENALTY=5.052133 PRIMER_INTERNAL_0_PENALTY=6.873727 PRIMER_LEFT_0_SEQUENCE=CAACATGGAACAGGGACAGA PRIMER_RIGHT_0_SEQUENCE=TCCTACCATATTTCATGCTCATTT PRIMER_INTERNAL_0_SEQUENCE=TCGTTAACTAAAGAGAAGAACTGGAA PRIMER_LEFT_0=117,20 PRIMER_RIGHT_0=193,24 PRIMER_INTERNAL_0=139,26 PRIMER_LEFT_0_TM=59.520 PRIMER_RIGHT_0_TM=58.948 PRIMER_INTERNAL_0_TM=59.126 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=33.333 PRIMER_INTERNAL_0_GC_PERCENT=34.615 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=5.587782 PRIMER_LEFT_1_PENALTY=0.480482 PRIMER_RIGHT_1_PENALTY=5.107300 PRIMER_INTERNAL_1_PENALTY=6.873727 PRIMER_LEFT_1_SEQUENCE=CAACATGGAACAGGGACAGA PRIMER_RIGHT_1_SEQUENCE=TCCTACCATATTTCATGCTCATTTT PRIMER_INTERNAL_1_SEQUENCE=TCGTTAACTAAAGAGAAGAACTGGAA PRIMER_LEFT_1=117,20 PRIMER_RIGHT_1=193,25 PRIMER_INTERNAL_1=139,26 PRIMER_LEFT_1_TM=59.520 PRIMER_RIGHT_1_TM=60.107 PRIMER_INTERNAL_1_TM=59.126 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=32.000 PRIMER_INTERNAL_1_GC_PERCENT=34.615 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.2000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=5.699135 PRIMER_LEFT_2_PENALTY=1.557510 PRIMER_RIGHT_2_PENALTY=4.141625 PRIMER_INTERNAL_2_PENALTY=4.708877 PRIMER_LEFT_2_SEQUENCE=GGAAAAAAATCTGCAACATGG PRIMER_RIGHT_2_SEQUENCE=TTTCATGCTCATTTTTAATTTCCA PRIMER_INTERNAL_2_SEQUENCE=AACAGGGACAGAAATCGTTAAC PRIMER_LEFT_2=104,21 PRIMER_RIGHT_2=183,24 PRIMER_INTERNAL_2=125,22 PRIMER_LEFT_2_TM=59.442 PRIMER_RIGHT_2_TM=59.858 PRIMER_INTERNAL_2_TM=57.291 PRIMER_LEFT_2_GC_PERCENT=38.095 PRIMER_RIGHT_2_GC_PERCENT=25.000 PRIMER_INTERNAL_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=5.784504 PRIMER_LEFT_3_PENALTY=4.776618 PRIMER_RIGHT_3_PENALTY=1.007886 PRIMER_INTERNAL_3_PENALTY=2.627407 PRIMER_LEFT_3_SEQUENCE=GCATTAAAATGGCTGATAAATCA PRIMER_RIGHT_3_SEQUENCE=ACGATTTCTGTCCCTGTTCC PRIMER_INTERNAL_3_SEQUENCE=CCCTAGGAAAAAAATCTGCAA PRIMER_LEFT_3=66,23 PRIMER_RIGHT_3=142,20 PRIMER_INTERNAL_3=99,21 PRIMER_LEFT_3_TM=58.223 PRIMER_RIGHT_3_TM=58.992 PRIMER_INTERNAL_3_TM=58.373 PRIMER_LEFT_3_GC_PERCENT=30.435 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=77 PRIMER_PAIR_4_PENALTY=5.793391 PRIMER_LEFT_4_PENALTY=0.480482 PRIMER_RIGHT_4_PENALTY=5.312909 PRIMER_INTERNAL_4_PENALTY=6.873727 PRIMER_LEFT_4_SEQUENCE=CAACATGGAACAGGGACAGA PRIMER_RIGHT_4_SEQUENCE=CCTACCATATTTCATGCTCATTTTT PRIMER_INTERNAL_4_SEQUENCE=TCGTTAACTAAAGAGAAGAACTGGAA PRIMER_LEFT_4=117,20 PRIMER_RIGHT_4=192,25 PRIMER_INTERNAL_4=139,26 PRIMER_LEFT_4_TM=59.520 PRIMER_RIGHT_4_TM=59.687 PRIMER_INTERNAL_4_TM=59.126 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=32.000 PRIMER_INTERNAL_4_GC_PERCENT=34.615 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH271 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTATTTTAAAAAAATTCTTATTTATTTATTTATTTATTTATTTATTTTGTAGAGANNAGGTCTTGTTACCTACCAGTCTGATCTTGAACTCCTGGCCACATGAAACTTATTAAAGGAAGTGACCATAATACTTTGTACAGTAGCATAGCACATTTATAATGCACATTGAACTTCAGGGGAATATATGAAAGTAAGAAGAAAATTTGCTTAGAACTTTCCTGCAGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCC SEQUENCE_INCLUDED_REGION=35,224 P3_COMMENT=CL is AGGTCTTGTTACCTACCAGTCTG P3_COMMENT=CR is TAAGCAAATTTTCTTCTTACTTTCA P3_COMMENT=CM is TCTTGAACTCCTGGCCACAT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.041150 PRIMER_LEFT_0_PENALTY=0.026532 PRIMER_RIGHT_0_PENALTY=1.014618 PRIMER_INTERNAL_0_PENALTY=8.234100 PRIMER_LEFT_0_SEQUENCE=AACTCCTGGCCACATGAAAC PRIMER_RIGHT_0_SEQUENCE=CCCTGAAGTTCAATGTGCATT PRIMER_INTERNAL_0_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT PRIMER_LEFT_0=123,20 PRIMER_RIGHT_0=214,21 PRIMER_INTERNAL_0=166,26 PRIMER_LEFT_0_TM=59.973 PRIMER_RIGHT_0_TM=59.985 PRIMER_INTERNAL_0_TM=57.766 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_INTERNAL_0_GC_PERCENT=34.615 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=8.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=92 PRIMER_PAIR_1_PENALTY=1.072499 PRIMER_LEFT_1_PENALTY=0.026532 PRIMER_RIGHT_1_PENALTY=1.045967 PRIMER_INTERNAL_1_PENALTY=8.234100 PRIMER_LEFT_1_SEQUENCE=AACTCCTGGCCACATGAAAC PRIMER_RIGHT_1_SEQUENCE=TCCCCTGAAGTTCAATGTGC PRIMER_INTERNAL_1_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT PRIMER_LEFT_1=123,20 PRIMER_RIGHT_1=216,20 PRIMER_INTERNAL_1=166,26 PRIMER_LEFT_1_TM=59.973 PRIMER_RIGHT_1_TM=61.046 PRIMER_INTERNAL_1_TM=57.766 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=34.615 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=94 PRIMER_PAIR_2_PENALTY=1.125695 PRIMER_LEFT_2_PENALTY=0.111077 PRIMER_RIGHT_2_PENALTY=1.014618 PRIMER_INTERNAL_2_PENALTY=8.234100 PRIMER_LEFT_2_SEQUENCE=CTCCTGGCCACATGAAACTT PRIMER_RIGHT_2_SEQUENCE=CCCTGAAGTTCAATGTGCATT PRIMER_INTERNAL_2_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT PRIMER_LEFT_2=125,20 PRIMER_RIGHT_2=214,21 PRIMER_INTERNAL_2=166,26 PRIMER_LEFT_2_TM=60.111 PRIMER_RIGHT_2_TM=59.985 PRIMER_INTERNAL_2_TM=57.766 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=34.615 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=8.4000 PRIMER_PAIR_2_COMPL_ANY=8.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=1.157044 PRIMER_LEFT_3_PENALTY=0.111077 PRIMER_RIGHT_3_PENALTY=1.045967 PRIMER_INTERNAL_3_PENALTY=8.234100 PRIMER_LEFT_3_SEQUENCE=CTCCTGGCCACATGAAACTT PRIMER_RIGHT_3_SEQUENCE=TCCCCTGAAGTTCAATGTGC PRIMER_INTERNAL_3_SEQUENCE=ACTTTGTACAGTAGCATAGCACATTT PRIMER_LEFT_3=125,20 PRIMER_RIGHT_3=216,20 PRIMER_INTERNAL_3=166,26 PRIMER_LEFT_3_TM=60.111 PRIMER_RIGHT_3_TM=61.046 PRIMER_INTERNAL_3_TM=57.766 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=34.615 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=8.00 PRIMER_PAIR_3_COMPL_END=7.00 PRIMER_PAIR_3_PRODUCT_SIZE=92 PRIMER_PAIR_4_PENALTY=1.353849 PRIMER_LEFT_4_PENALTY=0.339231 PRIMER_RIGHT_4_PENALTY=1.014618 PRIMER_INTERNAL_4_PENALTY=6.619157 PRIMER_LEFT_4_SEQUENCE=GATCTTGAACTCCTGGCCAC PRIMER_RIGHT_4_SEQUENCE=CCCTGAAGTTCAATGTGCATT PRIMER_INTERNAL_4_SEQUENCE=TGAAACTTATTAAAGGAAGTGACCA PRIMER_LEFT_4=116,20 PRIMER_RIGHT_4=214,21 PRIMER_INTERNAL_4=137,25 PRIMER_LEFT_4_TM=59.661 PRIMER_RIGHT_4_TM=59.985 PRIMER_INTERNAL_4_TM=58.381 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=32.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=8.4000 PRIMER_PAIR_4_COMPL_ANY=8.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = SEQUENCE_ID=MH274 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCAGTCACGTGTCACATGCAGTGTTTGCACTTGCCATTCATGGTTGTGAGGATTAAACAGACAAAAAGTNTACATAAAGAACCTAGCACGGCCACTGGCACGTAGTAGGAACACAAAAGAAAATGGTATTCCTTNTGATCTTTNTTATTTNATTTAGTCCTCATAATGCTTNTGTGNCAGCATTATCACCATTCCTGCACGAGGAAACAAGGCTTCAAAAGGATCAATAATTATGTAAACCCAAGGCTTGTGGGTGGCAGAGATCCGGCCGGAATTCA SEQUENCE_INCLUDED_REGION=35,281 P3_COMMENT=CL is CACATGCAGTGTTTGCACTT P3_COMMENT=CR is TTGATCCTTTTGAAGCCTTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.831026 PRIMER_LEFT_0_PENALTY=0.068789 PRIMER_RIGHT_0_PENALTY=0.762236 PRIMER_INTERNAL_0_PENALTY=0.119180 PRIMER_LEFT_0_SEQUENCE=CAGTCACGTGTCACATGCAG PRIMER_RIGHT_0_SEQUENCE=TGGCCGTGCTAGGTTCTTTA PRIMER_INTERNAL_0_SEQUENCE=GTTTGCACTTGCCATTCATG PRIMER_LEFT_0=39,20 PRIMER_RIGHT_0=131,20 PRIMER_INTERNAL_0=60,20 PRIMER_LEFT_0_TM=59.931 PRIMER_RIGHT_0_TM=60.762 PRIMER_INTERNAL_0_TM=60.119 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=6.3000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=93 PRIMER_PAIR_1_PENALTY=0.831026 PRIMER_LEFT_1_PENALTY=0.068789 PRIMER_RIGHT_1_PENALTY=0.762236 PRIMER_INTERNAL_1_PENALTY=0.119180 PRIMER_LEFT_1_SEQUENCE=CTGCAGTCACGTGTCACATG PRIMER_RIGHT_1_SEQUENCE=TGGCCGTGCTAGGTTCTTTA PRIMER_INTERNAL_1_SEQUENCE=GTTTGCACTTGCCATTCATG PRIMER_LEFT_1=36,20 PRIMER_RIGHT_1=131,20 PRIMER_INTERNAL_1=60,20 PRIMER_LEFT_1_TM=59.931 PRIMER_RIGHT_1_TM=60.762 PRIMER_INTERNAL_1_TM=60.119 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=7.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=6.3000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=0.984730 PRIMER_LEFT_2_PENALTY=0.222493 PRIMER_RIGHT_2_PENALTY=0.762236 PRIMER_INTERNAL_2_PENALTY=0.516718 PRIMER_LEFT_2_SEQUENCE=CGTGTCACATGCAGTGTTTG PRIMER_RIGHT_2_SEQUENCE=TGGCCGTGCTAGGTTCTTTA PRIMER_INTERNAL_2_SEQUENCE=TTGCCATTCATGGTTGTGAG PRIMER_LEFT_2=45,20 PRIMER_RIGHT_2=131,20 PRIMER_INTERNAL_2=68,20 PRIMER_LEFT_2_TM=59.778 PRIMER_RIGHT_2_TM=60.762 PRIMER_INTERNAL_2_TM=60.517 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=6.3000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=87 PRIMER_PAIR_3_PENALTY=0.990657 PRIMER_LEFT_3_PENALTY=0.228420 PRIMER_RIGHT_3_PENALTY=0.762236 PRIMER_INTERNAL_3_PENALTY=0.516718 PRIMER_LEFT_3_SEQUENCE=TCACGTGTCACATGCAGTGT PRIMER_RIGHT_3_SEQUENCE=TGGCCGTGCTAGGTTCTTTA PRIMER_INTERNAL_3_SEQUENCE=TTGCCATTCATGGTTGTGAG PRIMER_LEFT_3=42,20 PRIMER_RIGHT_3=131,20 PRIMER_INTERNAL_3=68,20 PRIMER_LEFT_3_TM=59.772 PRIMER_RIGHT_3_TM=60.762 PRIMER_INTERNAL_3_TM=60.517 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.1000 PRIMER_RIGHT_3_END_STABILITY=6.3000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=0.990657 PRIMER_LEFT_4_PENALTY=0.228420 PRIMER_RIGHT_4_PENALTY=0.762236 PRIMER_INTERNAL_4_PENALTY=0.516718 PRIMER_LEFT_4_SEQUENCE=GTCACGTGTCACATGCAGTG PRIMER_RIGHT_4_SEQUENCE=TGGCCGTGCTAGGTTCTTTA PRIMER_INTERNAL_4_SEQUENCE=TTGCCATTCATGGTTGTGAG PRIMER_LEFT_4=41,20 PRIMER_RIGHT_4=131,20 PRIMER_INTERNAL_4=68,20 PRIMER_LEFT_4_TM=59.772 PRIMER_RIGHT_4_TM=60.762 PRIMER_INTERNAL_4_TM=60.517 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.3000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH286 SEQUENCE_TEMPLATE=CTTCTTTTAAAAATCCTGTCAGCTGCAAAGGAAAGGAAGGACTAATATTTTCCACGAGATCAGTAACATCTTTATGAAGATGCTCCTTTTGAATGTGGTACAGAAAGTTGGAAAACACTTAGGCATGGTATCTGGCATAGTGCCCCTTAGGGAGCACTTCACATGCAGACGGTCTGGGGCTTAGGATGGTTTGACTTAGAATTTTTTGACTTTATGATAGTTGCAAAGGCGATGCACATTCAGTAGAAAGTGAACTTTGAGTCCCCATAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGT SEQUENCE_INCLUDED_REGION=0,271 P3_COMMENT=CL is AAATCCTGTCAGCTGCAAAG P3_COMMENT=CR is TTCTACTGAATGTGCATCGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.413785 PRIMER_LEFT_0_PENALTY=0.246438 PRIMER_RIGHT_0_PENALTY=0.167348 PRIMER_INTERNAL_0_PENALTY=0.327533 PRIMER_LEFT_0_SEQUENCE=CCCTTAGGGAGCACTTCACA PRIMER_RIGHT_0_SEQUENCE=CATCGCCTTTGCAACTATCA PRIMER_INTERNAL_0_SEQUENCE=GTCTGGGGCTTAGGATGGTT PRIMER_LEFT_0=143,20 PRIMER_RIGHT_0=233,20 PRIMER_INTERNAL_0=171,20 PRIMER_LEFT_0_TM=60.246 PRIMER_RIGHT_0_TM=59.833 PRIMER_INTERNAL_0_TM=60.328 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=5.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=91 PRIMER_PAIR_1_PENALTY=0.485929 PRIMER_LEFT_1_PENALTY=0.318581 PRIMER_RIGHT_1_PENALTY=0.167348 PRIMER_INTERNAL_1_PENALTY=0.692698 PRIMER_LEFT_1_SEQUENCE=CACTTCACATGCAGACGGTC PRIMER_RIGHT_1_SEQUENCE=CATCGCCTTTGCAACTATCA PRIMER_INTERNAL_1_SEQUENCE=GGGGCTTAGGATGGTTTGAC PRIMER_LEFT_1=154,20 PRIMER_RIGHT_1=233,20 PRIMER_INTERNAL_1=175,20 PRIMER_LEFT_1_TM=60.319 PRIMER_RIGHT_1_TM=59.833 PRIMER_INTERNAL_1_TM=60.693 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=5.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=0.488912 PRIMER_LEFT_2_PENALTY=0.318581 PRIMER_RIGHT_2_PENALTY=0.170331 PRIMER_INTERNAL_2_PENALTY=0.692698 PRIMER_LEFT_2_SEQUENCE=CACTTCACATGCAGACGGTC PRIMER_RIGHT_2_SEQUENCE=TCTACTGAATGTGCATCGCC PRIMER_INTERNAL_2_SEQUENCE=GGGGCTTAGGATGGTTTGAC PRIMER_LEFT_2=154,20 PRIMER_RIGHT_2=246,20 PRIMER_INTERNAL_2=175,20 PRIMER_LEFT_2_TM=60.319 PRIMER_RIGHT_2_TM=59.830 PRIMER_INTERNAL_2_TM=60.693 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.6000 PRIMER_RIGHT_2_END_STABILITY=11.4000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=93 PRIMER_PAIR_3_PENALTY=0.497864 PRIMER_LEFT_3_PENALTY=0.327533 PRIMER_RIGHT_3_PENALTY=0.170331 PRIMER_INTERNAL_3_PENALTY=7.511946 PRIMER_LEFT_3_SEQUENCE=GTCTGGGGCTTAGGATGGTT PRIMER_RIGHT_3_SEQUENCE=TCTACTGAATGTGCATCGCC PRIMER_INTERNAL_3_SEQUENCE=TTTTTTGACTTTATGATAGTTGCAAA PRIMER_LEFT_3=171,20 PRIMER_RIGHT_3=246,20 PRIMER_INTERNAL_3=201,26 PRIMER_LEFT_3_TM=60.328 PRIMER_RIGHT_3_TM=59.830 PRIMER_INTERNAL_3_TM=58.488 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=23.077 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=11.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=0.580576 PRIMER_LEFT_4_PENALTY=0.413229 PRIMER_RIGHT_4_PENALTY=0.167348 PRIMER_INTERNAL_4_PENALTY=0.327533 PRIMER_LEFT_4_SEQUENCE=TAGGGAGCACTTCACATGCA PRIMER_RIGHT_4_SEQUENCE=CATCGCCTTTGCAACTATCA PRIMER_INTERNAL_4_SEQUENCE=GTCTGGGGCTTAGGATGGTT PRIMER_LEFT_4=147,20 PRIMER_RIGHT_4=233,20 PRIMER_INTERNAL_4=171,20 PRIMER_LEFT_4_TM=60.413 PRIMER_RIGHT_4_TM=59.833 PRIMER_INTERNAL_4_TM=60.328 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=5.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH288 SEQUENCE_TEMPLATE=CTCCACTTCCCGGGTTCNTGCCATTGTCCTGCCTCAGCGTCCTGAATAGGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG SEQUENCE_INCLUDED_REGION=0,49 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH293 SEQUENCE_TEMPLATE=CTACTGGAGAGAGAACTGGGATTCAAATCCAGGAGCCCGCTCTTCACTCCAACACTATATTGCTTACTTAATGGTTACAGATTAAGCCCAGAAAGGAAGCCTGTCTCAATACACTAGATATAGTTACTGTGATTATATATTTTAATAAATGGTCCTTTTATTAAAAAAAAAAAAGNTATCTAAAGAGAAAACCATAATAATCTCTCAGGTAATTATGGCCACAGCCAAAACCAGTCTTTCTAAACCTAAAGACTCTCATAAAGGCCCTATCACATAACTTCTCCACTTCCCAGTAAAGCATTGAGG SEQUENCE_INCLUDED_REGION=0,306 P3_COMMENT=CL is CTCTTCACTCCAACACTATATTGC P3_COMMENT=CR is TGCTTTACTGGGAAGTGGAG P3_COMMENT=CM is TACTTAATGGTTACAGATTAAGCCC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.853930 PRIMER_LEFT_0_PENALTY=0.521793 PRIMER_RIGHT_0_PENALTY=0.332137 PRIMER_INTERNAL_0_PENALTY=0.444121 PRIMER_LEFT_0_SEQUENCE=CTGGGATTCAAATCCAGGAG PRIMER_RIGHT_0_SEQUENCE=AGGCTTCCTTTCTGGGCTTA PRIMER_INTERNAL_0_SEQUENCE=CCGCTCTTCACTCCAACACT PRIMER_LEFT_0=15,20 PRIMER_RIGHT_0=101,20 PRIMER_INTERNAL_0=36,20 PRIMER_LEFT_0_TM=59.478 PRIMER_RIGHT_0_TM=60.332 PRIMER_INTERNAL_0_TM=60.444 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=7.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=87 PRIMER_PAIR_1_PENALTY=0.995685 PRIMER_LEFT_1_PENALTY=0.663548 PRIMER_RIGHT_1_PENALTY=0.332137 PRIMER_INTERNAL_1_PENALTY=0.444121 PRIMER_LEFT_1_SEQUENCE=ACTGGGATTCAAATCCAGGA PRIMER_RIGHT_1_SEQUENCE=AGGCTTCCTTTCTGGGCTTA PRIMER_INTERNAL_1_SEQUENCE=CCGCTCTTCACTCCAACACT PRIMER_LEFT_1=14,20 PRIMER_RIGHT_1=101,20 PRIMER_INTERNAL_1=36,20 PRIMER_LEFT_1_TM=59.336 PRIMER_RIGHT_1_TM=60.332 PRIMER_INTERNAL_1_TM=60.444 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=88 PRIMER_PAIR_2_PENALTY=1.204747 PRIMER_LEFT_2_PENALTY=0.521793 PRIMER_RIGHT_2_PENALTY=0.682954 PRIMER_INTERNAL_2_PENALTY=0.444121 PRIMER_LEFT_2_SEQUENCE=CTGGGATTCAAATCCAGGAG PRIMER_RIGHT_2_SEQUENCE=GGCTTCCTTTCTGGGCTTAA PRIMER_INTERNAL_2_SEQUENCE=CCGCTCTTCACTCCAACACT PRIMER_LEFT_2=15,20 PRIMER_RIGHT_2=100,20 PRIMER_INTERNAL_2=36,20 PRIMER_LEFT_2_TM=59.478 PRIMER_RIGHT_2_TM=60.683 PRIMER_INTERNAL_2_TM=60.444 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.3000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=86 PRIMER_PAIR_3_PENALTY=1.277882 PRIMER_LEFT_3_PENALTY=0.521793 PRIMER_RIGHT_3_PENALTY=0.756089 PRIMER_INTERNAL_3_PENALTY=0.444121 PRIMER_LEFT_3_SEQUENCE=CTGGGATTCAAATCCAGGAG PRIMER_RIGHT_3_SEQUENCE=AGACAGGCTTCCTTTCTGGG PRIMER_INTERNAL_3_SEQUENCE=CCGCTCTTCACTCCAACACT PRIMER_LEFT_3=15,20 PRIMER_RIGHT_3=105,20 PRIMER_INTERNAL_3=36,20 PRIMER_LEFT_3_TM=59.478 PRIMER_RIGHT_3_TM=60.756 PRIMER_INTERNAL_3_TM=60.444 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=91 PRIMER_PAIR_4_PENALTY=1.287701 PRIMER_LEFT_4_PENALTY=0.148509 PRIMER_RIGHT_4_PENALTY=1.139192 PRIMER_INTERNAL_4_PENALTY=3.342564 PRIMER_LEFT_4_SEQUENCE=CCACAGCCAAAACCAGTCTT PRIMER_RIGHT_4_SEQUENCE=TGCTTTACTGGGAAGTGGAGA PRIMER_INTERNAL_4_SEQUENCE=CCTAAAGACTCTCATAAAGGCCC PRIMER_LEFT_4=218,20 PRIMER_RIGHT_4=299,21 PRIMER_INTERNAL_4=244,23 PRIMER_LEFT_4_TM=60.149 PRIMER_RIGHT_4_TM=59.861 PRIMER_INTERNAL_4_TM=59.657 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_INTERNAL_4_GC_PERCENT=47.826 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH295 SEQUENCE_TEMPLATE=CTGTGACCAATTGTTATTTTAGAGGGTTTAACAATGGCCTGACTATCACCTGATGGTCGCCAGAATTTCCTGGGGGAGGGCCTCCCCTGCCCTGATCATGTCTACCTAACTGCCTACTCTAACAATACTACTCCTGTGGTATGGGGATCCTAAGCCAAAAAGCTGAAATGAACATGTTCTAGCACTACAGAAATCCATACTGCCCCTCAGTAAAGGCAAATTTTAAATCTCTTTGGATAACCCAGGGCACATGTGAACAACCCCTTCTGAAGAAGCACTTATTCTTCTTCACACTCACCATGAAAA SEQUENCE_INCLUDED_REGION=0,306 P3_COMMENT=CL is TGTGACCAATTGTTATTTTAGAGG P3_COMMENT=CR is ATGGTGAGTGTGAAGAAGAATAA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.472761 PRIMER_LEFT_0_PENALTY=0.106310 PRIMER_RIGHT_0_PENALTY=0.366450 PRIMER_INTERNAL_0_PENALTY=0.816516 PRIMER_LEFT_0_SEQUENCE=GAAATCCATACTGCCCCTCA PRIMER_RIGHT_0_SEQUENCE=TGCTTCTTCAGAAGGGGTTG PRIMER_INTERNAL_0_SEQUENCE=CTCTTTGGATAACCCAGGGC PRIMER_LEFT_0=189,20 PRIMER_RIGHT_0=276,20 PRIMER_INTERNAL_0=228,20 PRIMER_LEFT_0_TM=59.894 PRIMER_RIGHT_0_TM=60.366 PRIMER_INTERNAL_0_TM=60.817 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 PRIMER_PAIR_1_PENALTY=0.483020 PRIMER_LEFT_1_PENALTY=0.376709 PRIMER_RIGHT_1_PENALTY=0.106310 PRIMER_INTERNAL_1_PENALTY=1.236972 PRIMER_LEFT_1_SEQUENCE=CCTGTGGTATGGGGATCCTA PRIMER_RIGHT_1_SEQUENCE=TGAGGGGCAGTATGGATTTC PRIMER_INTERNAL_1_SEQUENCE=GCCAAAAAGCTGAAATGAACA PRIMER_LEFT_1=132,20 PRIMER_RIGHT_1=208,20 PRIMER_INTERNAL_1=153,21 PRIMER_LEFT_1_TM=59.623 PRIMER_RIGHT_1_TM=59.894 PRIMER_INTERNAL_1_TM=60.237 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=0.491775 PRIMER_LEFT_2_PENALTY=0.040562 PRIMER_RIGHT_2_PENALTY=0.451213 PRIMER_INTERNAL_2_PENALTY=5.263882 PRIMER_LEFT_2_SEQUENCE=GGGATCCTAAGCCAAAAAGC PRIMER_RIGHT_2_SEQUENCE=ATTTGCCTTTACTGAGGGGC PRIMER_INTERNAL_2_SEQUENCE=CATGTTCTAGCACTACAGAAATCCA PRIMER_LEFT_2=143,20 PRIMER_RIGHT_2=220,20 PRIMER_INTERNAL_2=172,25 PRIMER_LEFT_2_TM=60.041 PRIMER_RIGHT_2_TM=60.451 PRIMER_INTERNAL_2_TM=59.736 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=12.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=78 PRIMER_PAIR_3_PENALTY=0.548000 PRIMER_LEFT_3_PENALTY=0.096788 PRIMER_RIGHT_3_PENALTY=0.451213 PRIMER_INTERNAL_3_PENALTY=5.263882 PRIMER_LEFT_3_SEQUENCE=GGGGATCCTAAGCCAAAAAG PRIMER_RIGHT_3_SEQUENCE=ATTTGCCTTTACTGAGGGGC PRIMER_INTERNAL_3_SEQUENCE=CATGTTCTAGCACTACAGAAATCCA PRIMER_LEFT_3=142,20 PRIMER_RIGHT_3=220,20 PRIMER_INTERNAL_3=172,25 PRIMER_LEFT_3_TM=59.903 PRIMER_RIGHT_3_TM=60.451 PRIMER_INTERNAL_3_TM=59.736 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=12.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=0.761112 PRIMER_LEFT_4_PENALTY=0.096788 PRIMER_RIGHT_4_PENALTY=0.664324 PRIMER_INTERNAL_4_PENALTY=5.263882 PRIMER_LEFT_4_SEQUENCE=GGGGATCCTAAGCCAAAAAG PRIMER_RIGHT_4_SEQUENCE=GCCTTTACTGAGGGGCAGTA PRIMER_INTERNAL_4_SEQUENCE=CATGTTCTAGCACTACAGAAATCCA PRIMER_LEFT_4=142,20 PRIMER_RIGHT_4=216,20 PRIMER_INTERNAL_4=172,25 PRIMER_LEFT_4_TM=59.903 PRIMER_RIGHT_4_TM=59.336 PRIMER_INTERNAL_4_TM=59.736 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=5.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH303 SEQUENCE_TEMPLATE=CTCACCATTACTTGCTCCACTGGGNCCCTGCAGGNATGCTGTGGTCCTCCCACTGGCAGGCTTCAGGGTACNGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCANCTGCATTAATGAATCGGCCAACGNGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCA SEQUENCE_INCLUDED_REGION=0,65 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH315 SEQUENCE_TEMPLATE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCCTAGCCATTAGGACTTCCTGAAGACAAGGCAGGCAGAACAAAAGAGAAAGAGCAAAGGTTCACAGGCAAGAAAGGACCTAGCAGCATGTCCAAGAAACAGNAAAAGGCTTGTGTGACTCAAGTGGGAAATCATGGGAAACAGGTCTGATATGAGGCTAAGGATCCAGGCAAAAGCCAGACTGTAAAGGGCAGT SEQUENCE_INCLUDED_REGION=31,193 P3_COMMENT=CL is GACTTCCTGAAGACAAGGCA P3_COMMENT=CR is TTTACAGTCTGGCTTTTGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.433504 PRIMER_LEFT_0_PENALTY=0.308849 PRIMER_RIGHT_0_PENALTY=0.124655 PRIMER_INTERNAL_0_PENALTY=0.566932 PRIMER_LEFT_0_SEQUENCE=CTCAAGTGGGAAATCATGGG PRIMER_RIGHT_0_SEQUENCE=TGCCCTTTACAGTCTGGCTT PRIMER_INTERNAL_0_SEQUENCE=ATGAGGCTAAGGATCCAGGC PRIMER_LEFT_0=147,20 PRIMER_RIGHT_0=221,20 PRIMER_INTERNAL_0=180,20 PRIMER_LEFT_0_TM=60.309 PRIMER_RIGHT_0_TM=59.875 PRIMER_INTERNAL_0_TM=60.567 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.6000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=0.464909 PRIMER_LEFT_1_PENALTY=0.156061 PRIMER_RIGHT_1_PENALTY=0.308849 PRIMER_INTERNAL_1_PENALTY=0.554836 PRIMER_LEFT_1_SEQUENCE=TCACAGGCAAGAAAGGACCT PRIMER_RIGHT_1_SEQUENCE=CCCATGATTTCCCACTTGAG PRIMER_INTERNAL_1_SEQUENCE=GCAGCATGTCCAAGAAACAG PRIMER_LEFT_1=90,20 PRIMER_RIGHT_1=166,20 PRIMER_INTERNAL_1=111,20 PRIMER_LEFT_1_TM=59.844 PRIMER_RIGHT_1_TM=60.309 PRIMER_INTERNAL_1_TM=59.445 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=0.541148 PRIMER_LEFT_2_PENALTY=0.232299 PRIMER_RIGHT_2_PENALTY=0.308849 PRIMER_INTERNAL_2_PENALTY=0.554836 PRIMER_LEFT_2_SEQUENCE=TTCACAGGCAAGAAAGGACC PRIMER_RIGHT_2_SEQUENCE=CCCATGATTTCCCACTTGAG PRIMER_INTERNAL_2_SEQUENCE=GCAGCATGTCCAAGAAACAG PRIMER_LEFT_2=89,20 PRIMER_RIGHT_2=166,20 PRIMER_INTERNAL_2=111,20 PRIMER_LEFT_2_TM=60.232 PRIMER_RIGHT_2_TM=60.309 PRIMER_INTERNAL_2_TM=59.445 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.1000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=78 PRIMER_PAIR_3_PENALTY=0.541148 PRIMER_LEFT_3_PENALTY=0.232299 PRIMER_RIGHT_3_PENALTY=0.308849 PRIMER_INTERNAL_3_PENALTY=0.554836 PRIMER_LEFT_3_SEQUENCE=GGTTCACAGGCAAGAAAGGA PRIMER_RIGHT_3_SEQUENCE=CCCATGATTTCCCACTTGAG PRIMER_INTERNAL_3_SEQUENCE=GCAGCATGTCCAAGAAACAG PRIMER_LEFT_3=87,20 PRIMER_RIGHT_3=166,20 PRIMER_INTERNAL_3=111,20 PRIMER_LEFT_3_TM=60.232 PRIMER_RIGHT_3_TM=60.309 PRIMER_INTERNAL_3_TM=59.445 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=0.558158 PRIMER_LEFT_4_PENALTY=0.003322 PRIMER_RIGHT_4_PENALTY=0.554836 PRIMER_INTERNAL_4_PENALTY=0.156061 PRIMER_LEFT_4_SEQUENCE=GAAGACAAGGCAGGCAGAAC PRIMER_RIGHT_4_SEQUENCE=TGTTTCTTGGACATGCTGCT PRIMER_INTERNAL_4_SEQUENCE=TCACAGGCAAGAAAGGACCT PRIMER_LEFT_4=50,20 PRIMER_RIGHT_4=129,20 PRIMER_INTERNAL_4=90,20 PRIMER_LEFT_4_TM=59.997 PRIMER_RIGHT_4_TM=59.445 PRIMER_INTERNAL_4_TM=59.844 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH318 SEQUENCE_TEMPLATE=TTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGGTTATTTTTCAGATTTGTTCATGTAGTTGCTTCGTAATGTCATTGGTATTTGCACTTAAGCGGAGGTAGTGGTTTTTCCTTTCCATATTTATTCATATTTATTATTTTCCTTTTTTTTTAAGTCACACATGAGTTTTTATTAAAGNCATATACTTACATTTTNAAAGCAAATATATTGACTAGTTCTTCTGAAAAATTGCTTGAAAAATGTAATTTTTTTT SEQUENCE_INCLUDED_REGION=32,224 P3_COMMENT=CL is TATTTGCACTTAAGCGGAGG P3_COMMENT=CR is ACATTTTTCAAGCAATTTTTCA P3_COMMENT=CM is AGTGGTTTTTCCTTTCCATATTT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.512761 PRIMER_LEFT_0_PENALTY=5.657140 PRIMER_RIGHT_0_PENALTY=0.855622 PRIMER_INTERNAL_0_PENALTY=2.773104 PRIMER_LEFT_0_SEQUENCE=TGGTTATTTTTCAGATTTGTTCATG PRIMER_RIGHT_0_SEQUENCE=GGAAAAACCACTACCTCCGC PRIMER_INTERNAL_0_SEQUENCE=TTGCTTCGTAATGTCATTGG PRIMER_LEFT_0=33,25 PRIMER_RIGHT_0=113,20 PRIMER_INTERNAL_0=61,20 PRIMER_LEFT_0_TM=59.343 PRIMER_RIGHT_0_TM=60.856 PRIMER_INTERNAL_0_TM=57.227 PRIMER_LEFT_0_GC_PERCENT=28.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=11.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=6.882352 PRIMER_LEFT_1_PENALTY=5.390387 PRIMER_RIGHT_1_PENALTY=1.491965 PRIMER_INTERNAL_1_PENALTY=3.252332 PRIMER_LEFT_1_SEQUENCE=TTTTCAGATTTGTTCATGTAGTTGC PRIMER_RIGHT_1_SEQUENCE=AAGGAAAAACCACTACCTCCG PRIMER_INTERNAL_1_SEQUENCE=TCGTAATGTCATTGGTATTTGCA PRIMER_LEFT_1=40,25 PRIMER_RIGHT_1=115,21 PRIMER_INTERNAL_1=66,23 PRIMER_LEFT_1_TM=59.610 PRIMER_RIGHT_1_TM=59.508 PRIMER_INTERNAL_1_TM=60.252 PRIMER_LEFT_1_GC_PERCENT=32.000 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_INTERNAL_1_GC_PERCENT=34.783 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=9.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=6.978959 PRIMER_LEFT_2_PENALTY=1.136289 PRIMER_RIGHT_2_PENALTY=5.842670 PRIMER_INTERNAL_2_PENALTY=2.914618 PRIMER_LEFT_2_SEQUENCE=TTGGTATTTGCACTTAAGCGG PRIMER_RIGHT_2_SEQUENCE=TCATGTGTGACTTAAAAAAAAAGGA PRIMER_INTERNAL_2_SEQUENCE=GGTAGTGGTTTTTCCTTTCCA PRIMER_LEFT_2=77,21 PRIMER_RIGHT_2=166,25 PRIMER_INTERNAL_2=99,21 PRIMER_LEFT_2_TM=60.136 PRIMER_RIGHT_2_TM=59.157 PRIMER_INTERNAL_2_TM=58.085 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=28.000 PRIMER_INTERNAL_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=11.4000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=6.982128 PRIMER_LEFT_3_PENALTY=1.139458 PRIMER_RIGHT_3_PENALTY=5.842670 PRIMER_INTERNAL_3_PENALTY=5.718597 PRIMER_LEFT_3_SEQUENCE=ATTTGCACTTAAGCGGAGGT PRIMER_RIGHT_3_SEQUENCE=TCATGTGTGACTTAAAAAAAAAGGA PRIMER_INTERNAL_3_SEQUENCE=AGTGGTTTTTCCTTTCCATATTT PRIMER_LEFT_3=82,20 PRIMER_RIGHT_3=166,25 PRIMER_INTERNAL_3=102,23 PRIMER_LEFT_3_TM=58.861 PRIMER_RIGHT_3_TM=59.157 PRIMER_INTERNAL_3_TM=57.281 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=28.000 PRIMER_INTERNAL_3_GC_PERCENT=30.435 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=85 PRIMER_PAIR_4_PENALTY=7.001609 PRIMER_LEFT_4_PENALTY=1.158939 PRIMER_RIGHT_4_PENALTY=5.842670 PRIMER_INTERNAL_4_PENALTY=2.914618 PRIMER_LEFT_4_SEQUENCE=TGGTATTTGCACTTAAGCGG PRIMER_RIGHT_4_SEQUENCE=TCATGTGTGACTTAAAAAAAAAGGA PRIMER_INTERNAL_4_SEQUENCE=GGTAGTGGTTTTTCCTTTCCA PRIMER_LEFT_4=78,20 PRIMER_RIGHT_4=166,25 PRIMER_INTERNAL_4=99,21 PRIMER_LEFT_4_TM=58.841 PRIMER_RIGHT_4_TM=59.157 PRIMER_INTERNAL_4_TM=58.085 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=28.000 PRIMER_INTERNAL_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=11.4000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH319 SEQUENCE_TEMPLATE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCTGAGAATCAAATCAAGAACTCAACCCCTTTCACAATAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGNGCGGGGAGAGGCGGTTT SEQUENCE_INCLUDED_REGION=31,37 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH323 SEQUENCE_TEMPLATE=TCATGCCTGAGGTCGACTCTAGAGGATCCCCCTTATTTTGAAAAATAGTTCATATATTTATAAACATCTTTTAATTCCATTTTTCCATGAACTTTTTGAACTCCTCATATTCTCTATATCTNTNTTTAAAAATTTACAAAAATAAAAACTTTTAAAAGTTATAAAAGAAATATTTTCATGTCAGATATAGGAAAAAATACAACANGGTCACCTAGAATTTTACCAGAACCACGTAGCAACCTCTAATCATTTTGTGTGCTTAATTTTAGTCATTTTTATTATTCTTAAGATGTTAAATAATTTAAA SEQUENCE_INCLUDED_REGION=31,275 P3_COMMENT=CL is AACATCTTTTAATTCCATTTTTCC P3_COMMENT=CR is CAAAATGATTAGAGGTTGCTACG P3_COMMENT=CM is TGAACTTTTTGAACTCCTCATATTC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=7.269945 PRIMER_LEFT_0_PENALTY=3.376837 PRIMER_RIGHT_0_PENALTY=3.893108 PRIMER_INTERNAL_0_PENALTY=9.512142 PRIMER_LEFT_0_SEQUENCE=TCCATGAACTTTTTGAACTCCTC PRIMER_RIGHT_0_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGG PRIMER_INTERNAL_0_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA PRIMER_LEFT_0=83,23 PRIMER_RIGHT_0=232,23 PRIMER_INTERNAL_0=175,27 PRIMER_LEFT_0_TM=59.623 PRIMER_RIGHT_0_TM=59.107 PRIMER_INTERNAL_0_TM=57.488 PRIMER_LEFT_0_GC_PERCENT=39.130 PRIMER_RIGHT_0_GC_PERCENT=43.478 PRIMER_INTERNAL_0_GC_PERCENT=25.926 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=150 PRIMER_PAIR_1_PENALTY=7.392962 PRIMER_LEFT_1_PENALTY=3.499854 PRIMER_RIGHT_1_PENALTY=3.893108 PRIMER_INTERNAL_1_PENALTY=9.512142 PRIMER_LEFT_1_SEQUENCE=CCATGAACTTTTTGAACTCCTCA PRIMER_RIGHT_1_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGG PRIMER_INTERNAL_1_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA PRIMER_LEFT_1=84,23 PRIMER_RIGHT_1=232,23 PRIMER_INTERNAL_1=175,27 PRIMER_LEFT_1_TM=60.500 PRIMER_RIGHT_1_TM=59.107 PRIMER_INTERNAL_1_TM=57.488 PRIMER_LEFT_1_GC_PERCENT=39.130 PRIMER_RIGHT_1_GC_PERCENT=43.478 PRIMER_INTERNAL_1_GC_PERCENT=25.926 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.2000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=149 PRIMER_PAIR_2_PENALTY=7.537689 PRIMER_LEFT_2_PENALTY=3.376837 PRIMER_RIGHT_2_PENALTY=4.160852 PRIMER_INTERNAL_2_PENALTY=9.512142 PRIMER_LEFT_2_SEQUENCE=TCCATGAACTTTTTGAACTCCTC PRIMER_RIGHT_2_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGGT PRIMER_INTERNAL_2_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA PRIMER_LEFT_2=83,23 PRIMER_RIGHT_2=232,24 PRIMER_INTERNAL_2=175,27 PRIMER_LEFT_2_TM=59.623 PRIMER_RIGHT_2_TM=59.839 PRIMER_INTERNAL_2_TM=57.488 PRIMER_LEFT_2_GC_PERCENT=39.130 PRIMER_RIGHT_2_GC_PERCENT=41.667 PRIMER_INTERNAL_2_GC_PERCENT=25.926 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=150 PRIMER_PAIR_3_PENALTY=7.660707 PRIMER_LEFT_3_PENALTY=3.499854 PRIMER_RIGHT_3_PENALTY=4.160852 PRIMER_INTERNAL_3_PENALTY=9.512142 PRIMER_LEFT_3_SEQUENCE=CCATGAACTTTTTGAACTCCTCA PRIMER_RIGHT_3_SEQUENCE=CGTGGTTCTGGTAAAATTCTAGGT PRIMER_INTERNAL_3_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA PRIMER_LEFT_3=84,23 PRIMER_RIGHT_3=232,24 PRIMER_INTERNAL_3=175,27 PRIMER_LEFT_3_TM=60.500 PRIMER_RIGHT_3_TM=59.839 PRIMER_INTERNAL_3_TM=57.488 PRIMER_LEFT_3_GC_PERCENT=39.130 PRIMER_RIGHT_3_GC_PERCENT=41.667 PRIMER_INTERNAL_3_GC_PERCENT=25.926 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=149 PRIMER_PAIR_4_PENALTY=7.660707 PRIMER_LEFT_4_PENALTY=3.499854 PRIMER_RIGHT_4_PENALTY=4.160852 PRIMER_INTERNAL_4_PENALTY=9.512142 PRIMER_LEFT_4_SEQUENCE=CCATGAACTTTTTGAACTCCTCA PRIMER_RIGHT_4_SEQUENCE=ACGTGGTTCTGGTAAAATTCTAGG PRIMER_INTERNAL_4_SEQUENCE=TCATGTCAGATATAGGAAAAAATACAA PRIMER_LEFT_4=84,23 PRIMER_RIGHT_4=233,24 PRIMER_INTERNAL_4=175,27 PRIMER_LEFT_4_TM=60.500 PRIMER_RIGHT_4_TM=59.839 PRIMER_INTERNAL_4_TM=57.488 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=41.667 PRIMER_INTERNAL_4_GC_PERCENT=25.926 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=7.2000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=150 = SEQUENCE_ID=MH328 SEQUENCE_TEMPLATE=NCTTCATGCTNAGGTCGACTCTAGAGGATCCCCTAATTAACATATGCATTATCTCACTTATCTCTTTTTTGTGGTGAGAACACTTAAAATCTAAGAATGATCAATTTCAAATAAAGATGGTAGTGAGCGAACAGAAGAGGTTTCATTGACTCCTAAACTGAGTACTCAAAAACGAGCAGGTGCTCACAGTCAGGAAGCAGGTGCTGAGTACAGGATGGGAAAAAGCATGGGGGTGTGCACTTGTCAAGAAAATGACAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCG SEQUENCE_INCLUDED_REGION=33,190 P3_COMMENT=CL is TCACTTATCTCTTTTTTGTGGTG P3_COMMENT=CR is ATCCTGTACTCAGCACCTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.415468 PRIMER_LEFT_0_PENALTY=1.393460 PRIMER_RIGHT_0_PENALTY=0.022009 PRIMER_INTERNAL_0_PENALTY=0.440316 PRIMER_LEFT_0_SEQUENCE=GAGCGAACAGAAGAGGTTTCA PRIMER_RIGHT_0_SEQUENCE=CACCTGCTTCCTGACTGTGA PRIMER_INTERNAL_0_SEQUENCE=ACTCAAAAACGAGCAGGTGC PRIMER_LEFT_0=124,21 PRIMER_RIGHT_0=202,20 PRIMER_INTERNAL_0=163,20 PRIMER_LEFT_0_TM=59.607 PRIMER_RIGHT_0_TM=60.022 PRIMER_INTERNAL_0_TM=60.440 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=79 PRIMER_PAIR_1_PENALTY=1.415468 PRIMER_LEFT_1_PENALTY=1.393460 PRIMER_RIGHT_1_PENALTY=0.022009 PRIMER_INTERNAL_1_PENALTY=0.440316 PRIMER_LEFT_1_SEQUENCE=TGAGCGAACAGAAGAGGTTTC PRIMER_RIGHT_1_SEQUENCE=CACCTGCTTCCTGACTGTGA PRIMER_INTERNAL_1_SEQUENCE=ACTCAAAAACGAGCAGGTGC PRIMER_LEFT_1=123,21 PRIMER_RIGHT_1=202,20 PRIMER_INTERNAL_1=163,20 PRIMER_LEFT_1_TM=59.607 PRIMER_RIGHT_1_TM=60.022 PRIMER_INTERNAL_1_TM=60.440 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=1.450346 PRIMER_LEFT_2_PENALTY=1.393460 PRIMER_RIGHT_2_PENALTY=0.056887 PRIMER_INTERNAL_2_PENALTY=0.440316 PRIMER_LEFT_2_SEQUENCE=GAGCGAACAGAAGAGGTTTCA PRIMER_RIGHT_2_SEQUENCE=AGCACCTGCTTCCTGACTGT PRIMER_INTERNAL_2_SEQUENCE=ACTCAAAAACGAGCAGGTGC PRIMER_LEFT_2=124,21 PRIMER_RIGHT_2=204,20 PRIMER_INTERNAL_2=163,20 PRIMER_LEFT_2_TM=59.607 PRIMER_RIGHT_2_TM=60.057 PRIMER_INTERNAL_2_TM=60.440 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=6.1000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=1.450346 PRIMER_LEFT_3_PENALTY=1.393460 PRIMER_RIGHT_3_PENALTY=0.056887 PRIMER_INTERNAL_3_PENALTY=0.440316 PRIMER_LEFT_3_SEQUENCE=TGAGCGAACAGAAGAGGTTTC PRIMER_RIGHT_3_SEQUENCE=AGCACCTGCTTCCTGACTGT PRIMER_INTERNAL_3_SEQUENCE=ACTCAAAAACGAGCAGGTGC PRIMER_LEFT_3=123,21 PRIMER_RIGHT_3=204,20 PRIMER_INTERNAL_3=163,20 PRIMER_LEFT_3_TM=59.607 PRIMER_RIGHT_3_TM=60.057 PRIMER_INTERNAL_3_TM=60.440 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=82 PRIMER_PAIR_4_PENALTY=1.580464 PRIMER_LEFT_4_PENALTY=1.393460 PRIMER_RIGHT_4_PENALTY=0.187005 PRIMER_INTERNAL_4_PENALTY=4.466328 PRIMER_LEFT_4_SEQUENCE=GAGCGAACAGAAGAGGTTTCA PRIMER_RIGHT_4_SEQUENCE=TGCTTCCTGACTGTGAGCAC PRIMER_INTERNAL_4_SEQUENCE=AAACTGAGTACTCAAAAACGAGCA PRIMER_LEFT_4=124,21 PRIMER_RIGHT_4=198,20 PRIMER_INTERNAL_4=154,24 PRIMER_LEFT_4_TM=59.607 PRIMER_RIGHT_4_TM=60.187 PRIMER_INTERNAL_4_TM=59.534 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=37.500 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=10.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH331 SEQUENCE_TEMPLATE=TTCATGCTGAGGTCGACTCTAGAGGATCCCCCTATACATTTNAATATTGACTGCAATTGCACGTCCTATATCAATACTCACCTGTGTTTTTTAAACTGTGAACCAGTAAAGCATGATGATGATTGGCATCCCTCTGCAGTTAAAAAGAAGCCTAATAGTGTTAAAAATTCAATTCATCCAACACAAAGTTCTAAAATTTTNATGCCTTATAGAATTACTTGAGCACTCTCCAAAATCCCATATCCATGTCACAACCAATATTAAGTCCAAATATAACCAGTCCTTTTCTTTTTAATAGTCTTCATTTTTTTANGAGCAGTTTGAAATCCACAGCAAAGGGGTACCGA SEQUENCE_INCLUDED_REGION=31,275 P3_COMMENT=CL is ACTGCAATTGCACGTCCTAT P3_COMMENT=CR is AGACTATTAAAAAGAAAAGGACTGG P3_COMMENT=CM is TGTTTTTTAAACTGTGAACCAGTAA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.169955 PRIMER_LEFT_0_PENALTY=0.129070 PRIMER_RIGHT_0_PENALTY=0.040885 PRIMER_INTERNAL_0_PENALTY=4.945226 PRIMER_LEFT_0_SEQUENCE=GACTGCAATTGCACGTCCTA PRIMER_RIGHT_0_SEQUENCE=TGCCAATCATCATCATGCTT PRIMER_INTERNAL_0_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC PRIMER_LEFT_0=48,20 PRIMER_RIGHT_0=127,20 PRIMER_INTERNAL_0=80,24 PRIMER_LEFT_0_TM=59.871 PRIMER_RIGHT_0_TM=60.041 PRIMER_INTERNAL_0_TM=59.055 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=37.500 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=8.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=0.273745 PRIMER_LEFT_1_PENALTY=0.129070 PRIMER_RIGHT_1_PENALTY=0.144675 PRIMER_INTERNAL_1_PENALTY=4.945226 PRIMER_LEFT_1_SEQUENCE=GACTGCAATTGCACGTCCTA PRIMER_RIGHT_1_SEQUENCE=GATGCCAATCATCATCATGC PRIMER_INTERNAL_1_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC PRIMER_LEFT_1=48,20 PRIMER_RIGHT_1=129,20 PRIMER_INTERNAL_1=80,24 PRIMER_LEFT_1_TM=59.871 PRIMER_RIGHT_1_TM=59.855 PRIMER_INTERNAL_1_TM=59.055 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=0.342575 PRIMER_LEFT_2_PENALTY=0.129070 PRIMER_RIGHT_2_PENALTY=0.213506 PRIMER_INTERNAL_2_PENALTY=3.010529 PRIMER_LEFT_2_SEQUENCE=GACTGCAATTGCACGTCCTA PRIMER_RIGHT_2_SEQUENCE=TTTAACTGCAGAGGGATGCC PRIMER_INTERNAL_2_SEQUENCE=TGAACCAGTAAAGCATGATGATG PRIMER_LEFT_2=48,20 PRIMER_RIGHT_2=143,20 PRIMER_INTERNAL_2=98,23 PRIMER_LEFT_2_TM=59.871 PRIMER_RIGHT_2_TM=60.214 PRIMER_INTERNAL_2_TM=60.011 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=12.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=9.6000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=0.381318 PRIMER_LEFT_3_PENALTY=0.129070 PRIMER_RIGHT_3_PENALTY=0.252249 PRIMER_INTERNAL_3_PENALTY=4.945226 PRIMER_LEFT_3_SEQUENCE=GACTGCAATTGCACGTCCTA PRIMER_RIGHT_3_SEQUENCE=GAGGGATGCCAATCATCATC PRIMER_INTERNAL_3_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC PRIMER_LEFT_3=48,20 PRIMER_RIGHT_3=133,20 PRIMER_INTERNAL_3=80,24 PRIMER_LEFT_3_TM=59.871 PRIMER_RIGHT_3_TM=60.252 PRIMER_INTERNAL_3_TM=59.055 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=37.500 PRIMER_LEFT_3_SELF_ANY=12.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=86 PRIMER_PAIR_4_PENALTY=0.434388 PRIMER_LEFT_4_PENALTY=0.129070 PRIMER_RIGHT_4_PENALTY=0.305318 PRIMER_INTERNAL_4_PENALTY=4.945226 PRIMER_LEFT_4_SEQUENCE=GACTGCAATTGCACGTCCTA PRIMER_RIGHT_4_SEQUENCE=GGATGCCAATCATCATCATG PRIMER_INTERNAL_4_SEQUENCE=CCTGTGTTTTTTAAACTGTGAACC PRIMER_LEFT_4=48,20 PRIMER_RIGHT_4=130,20 PRIMER_INTERNAL_4=80,24 PRIMER_LEFT_4_TM=59.871 PRIMER_RIGHT_4_TM=59.695 PRIMER_INTERNAL_4_TM=59.055 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=37.500 PRIMER_LEFT_4_SELF_ANY=12.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=83 = SEQUENCE_ID=MH333 SEQUENCE_TEMPLATE=NNCCANNNGGTCGACTCTAGAGGATCCCCCTAATCCTGATCTATTCAAATCTTAACAACTTAAGTTGATGGAGTNGACTTCTTCTAAATTAGTNATTCCCTAATTTGCCTGACTGTTGGAATTTCCAGGGCATGTTGAAAATACACATTATCCAGACTCTTACCTCTGCAGATCTATTTAGTGGTTCTAAAATGGCAGCCAGGAGTCTGGATTTTTCCCAGGGGTCCTGTGTAATTCACACTGATGAACAGGCAAGTTTGGGAAATAGTGCCTTAAGGAGATTTTTCATTAAGCAGTCTTCATTTGAAATGAGG SEQUENCE_INCLUDED_REGION=29,285 P3_COMMENT=CL is ATCCTGATCTATTCAAATCTTAACA P3_COMMENT=CR is AAGACTGCTTAATGAAAAATCTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.301918 PRIMER_LEFT_0_PENALTY=0.213506 PRIMER_RIGHT_0_PENALTY=0.088413 PRIMER_INTERNAL_0_PENALTY=0.740815 PRIMER_LEFT_0_SEQUENCE=TAAAATGGCAGCCAGGAGTC PRIMER_RIGHT_0_SEQUENCE=TTTCCCAAACTTGCCTGTTC PRIMER_INTERNAL_0_SEQUENCE=CCAGGGGTCCTGTGTAATTC PRIMER_LEFT_0=187,20 PRIMER_RIGHT_0=264,20 PRIMER_INTERNAL_0=217,20 PRIMER_LEFT_0_TM=60.214 PRIMER_RIGHT_0_TM=60.088 PRIMER_INTERNAL_0_TM=59.259 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.1000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 PRIMER_PAIR_1_PENALTY=0.380827 PRIMER_LEFT_1_PENALTY=0.292414 PRIMER_RIGHT_1_PENALTY=0.088413 PRIMER_INTERNAL_1_PENALTY=0.298276 PRIMER_LEFT_1_SEQUENCE=GTTCTAAAATGGCAGCCAGG PRIMER_RIGHT_1_SEQUENCE=TTTCCCAAACTTGCCTGTTC PRIMER_INTERNAL_1_SEQUENCE=AGTCTGGATTTTTCCCAGGG PRIMER_LEFT_1=183,20 PRIMER_RIGHT_1=264,20 PRIMER_INTERNAL_1=203,20 PRIMER_LEFT_1_TM=59.708 PRIMER_RIGHT_1_TM=60.088 PRIMER_INTERNAL_1_TM=60.298 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=0.380827 PRIMER_LEFT_2_PENALTY=0.292414 PRIMER_RIGHT_2_PENALTY=0.088413 PRIMER_INTERNAL_2_PENALTY=0.298276 PRIMER_LEFT_2_SEQUENCE=GGTTCTAAAATGGCAGCCAG PRIMER_RIGHT_2_SEQUENCE=TTTCCCAAACTTGCCTGTTC PRIMER_INTERNAL_2_SEQUENCE=AGTCTGGATTTTTCCCAGGG PRIMER_LEFT_2=182,20 PRIMER_RIGHT_2=264,20 PRIMER_INTERNAL_2=203,20 PRIMER_LEFT_2_TM=59.708 PRIMER_RIGHT_2_TM=60.088 PRIMER_INTERNAL_2_TM=60.298 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=0.432363 PRIMER_LEFT_3_PENALTY=0.343950 PRIMER_RIGHT_3_PENALTY=0.088413 PRIMER_INTERNAL_3_PENALTY=0.740815 PRIMER_LEFT_3_SEQUENCE=TCTAAAATGGCAGCCAGGAG PRIMER_RIGHT_3_SEQUENCE=TTTCCCAAACTTGCCTGTTC PRIMER_INTERNAL_3_SEQUENCE=CCAGGGGTCCTGTGTAATTC PRIMER_LEFT_3=185,20 PRIMER_RIGHT_3=264,20 PRIMER_INTERNAL_3=217,20 PRIMER_LEFT_3_TM=60.344 PRIMER_RIGHT_3_TM=60.088 PRIMER_INTERNAL_3_TM=59.259 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=0.514222 PRIMER_LEFT_4_PENALTY=0.425809 PRIMER_RIGHT_4_PENALTY=0.088413 PRIMER_INTERNAL_4_PENALTY=0.298276 PRIMER_LEFT_4_SEQUENCE=GTGGTTCTAAAATGGCAGCC PRIMER_RIGHT_4_SEQUENCE=TTTCCCAAACTTGCCTGTTC PRIMER_INTERNAL_4_SEQUENCE=AGTCTGGATTTTTCCCAGGG PRIMER_LEFT_4=180,20 PRIMER_RIGHT_4=264,20 PRIMER_INTERNAL_4=203,20 PRIMER_LEFT_4_TM=59.574 PRIMER_RIGHT_4_TM=60.088 PRIMER_INTERNAL_4_TM=60.298 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=85 = SEQUENCE_ID=MH335 SEQUENCE_TEMPLATE=NATGCTGAGGTCGACTCTAGAGGATCCCCTCAGATTTCTTCTCTACGTTGGTTAATGAGTNCCTTTTCAAACGGTTATATTTTGGGGTTGAGGTAAAGGTTTTTAGAGTNNTTCCAATCGTGTATGTAAGCAGTCAACTATTTNCCCTATGTGTAGGTCTGGCACCACAGACCTGTAGAAGTCTTCTCTTTAATTGTACCATTATTTTGAATGTAAAAAAAAANCAANTAAACATGTTGTTGTCATTGANCTGGCTGTAGATGCATACATAAG SEQUENCE_INCLUDED_REGION=29,244 P3_COMMENT=CL is TCAGATTTCTTCTCTACGTTGG P3_COMMENT=CR is TTCTACAGGTCTGTGGTGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.387185 PRIMER_LEFT_0_PENALTY=1.077784 PRIMER_RIGHT_0_PENALTY=0.309402 PRIMER_INTERNAL_0_PENALTY=3.067516 PRIMER_LEFT_0_SEQUENCE=TTTTGGGGTTGAGGTAAAGG PRIMER_RIGHT_0_SEQUENCE=TCTGTGGTGCCAGACCTACA PRIMER_INTERNAL_0_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA PRIMER_LEFT_0=79,20 PRIMER_RIGHT_0=170,20 PRIMER_INTERNAL_0=113,23 PRIMER_LEFT_0_TM=58.922 PRIMER_RIGHT_0_TM=60.309 PRIMER_INTERNAL_0_TM=60.068 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=5.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=92 PRIMER_PAIR_1_PENALTY=1.387185 PRIMER_LEFT_1_PENALTY=1.077784 PRIMER_RIGHT_1_PENALTY=0.309402 PRIMER_INTERNAL_1_PENALTY=3.067516 PRIMER_LEFT_1_SEQUENCE=TTTTGGGGTTGAGGTAAAGG PRIMER_RIGHT_1_SEQUENCE=TACAGGTCTGTGGTGCCAGA PRIMER_INTERNAL_1_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA PRIMER_LEFT_1=79,20 PRIMER_RIGHT_1=176,20 PRIMER_INTERNAL_1=113,23 PRIMER_LEFT_1_TM=58.922 PRIMER_RIGHT_1_TM=60.309 PRIMER_INTERNAL_1_TM=60.068 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=43.478 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=98 PRIMER_PAIR_2_PENALTY=1.387185 PRIMER_LEFT_2_PENALTY=1.077784 PRIMER_RIGHT_2_PENALTY=0.309402 PRIMER_INTERNAL_2_PENALTY=3.067516 PRIMER_LEFT_2_SEQUENCE=TTTTGGGGTTGAGGTAAAGG PRIMER_RIGHT_2_SEQUENCE=TCTACAGGTCTGTGGTGCCA PRIMER_INTERNAL_2_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA PRIMER_LEFT_2=79,20 PRIMER_RIGHT_2=178,20 PRIMER_INTERNAL_2=113,23 PRIMER_LEFT_2_TM=58.922 PRIMER_RIGHT_2_TM=60.309 PRIMER_INTERNAL_2_TM=60.068 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=1.581709 PRIMER_LEFT_3_PENALTY=1.272307 PRIMER_RIGHT_3_PENALTY=0.309402 PRIMER_INTERNAL_3_PENALTY=3.067516 PRIMER_LEFT_3_SEQUENCE=TGGGGTTGAGGTAAAGGTTTT PRIMER_RIGHT_3_SEQUENCE=TCTGTGGTGCCAGACCTACA PRIMER_INTERNAL_3_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA PRIMER_LEFT_3=82,21 PRIMER_RIGHT_3=170,20 PRIMER_INTERNAL_3=113,23 PRIMER_LEFT_3_TM=59.728 PRIMER_RIGHT_3_TM=60.309 PRIMER_INTERNAL_3_TM=60.068 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=5.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=89 PRIMER_PAIR_4_PENALTY=1.581709 PRIMER_LEFT_4_PENALTY=1.272307 PRIMER_RIGHT_4_PENALTY=0.309402 PRIMER_INTERNAL_4_PENALTY=3.067516 PRIMER_LEFT_4_SEQUENCE=TGGGGTTGAGGTAAAGGTTTT PRIMER_RIGHT_4_SEQUENCE=TACAGGTCTGTGGTGCCAGA PRIMER_INTERNAL_4_SEQUENCE=CCAATCGTGTATGTAAGCAGTCA PRIMER_LEFT_4=82,21 PRIMER_RIGHT_4=176,20 PRIMER_INTERNAL_4=113,23 PRIMER_LEFT_4_TM=59.728 PRIMER_RIGHT_4_TM=60.309 PRIMER_INTERNAL_4_TM=60.068 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=95 = SEQUENCE_ID=MH336 SEQUENCE_TEMPLATE=GCCTGCAGGTCGACTCTAGAGGATCCCACCTATCCCATTACGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=27,14 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH341 SEQUENCE_TEMPLATE=CGTTCATGCTCAGGTCGACTCTAGAGGATCCCCCTCCTTGAGGNCAAGTACAGCATGAAGGAATAATACTGTGGAAGTATAAACAGCAGGCTCCAGGCTCATCAGAAGAAAGCCTTACTGTGTACTTCTCTTTAAAAAAAAATATGTGAAATCAGATATTTATAGGAATTAGATATGTATTTNCATATTGATACATTTTGTGTATTATATGGGATTCCTGATATATCTATGTAGTATGGTTTCTNATACATAANNTATCTGATTTCATATGT SEQUENCE_INCLUDED_REGION=33,239 P3_COMMENT=CL is ACTGTGGAAGTATAAACAGCAGG P3_COMMENT=CR is ATCAGGAATCCCATATAATACACA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=11.127070 PRIMER_LEFT_0_PENALTY=4.081412 PRIMER_RIGHT_0_PENALTY=7.045658 PRIMER_INTERNAL_0_PENALTY=1.664117 PRIMER_LEFT_0_SEQUENCE=CAGCATGAAGGAATAATACTGTGG PRIMER_RIGHT_0_SEQUENCE=AGTACACAGTAAGGCTTTCTTCTGA PRIMER_INTERNAL_0_SEQUENCE=ATAAACAGCAGGCTCCAGGC PRIMER_LEFT_0=50,24 PRIMER_RIGHT_0=125,25 PRIMER_INTERNAL_0=78,20 PRIMER_LEFT_0_TM=59.919 PRIMER_RIGHT_0_TM=57.954 PRIMER_INTERNAL_0_TM=61.664 PRIMER_LEFT_0_GC_PERCENT=41.667 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=11.522908 PRIMER_LEFT_1_PENALTY=4.081412 PRIMER_RIGHT_1_PENALTY=7.441496 PRIMER_INTERNAL_1_PENALTY=1.664117 PRIMER_LEFT_1_SEQUENCE=CAGCATGAAGGAATAATACTGTGG PRIMER_RIGHT_1_SEQUENCE=AAGTACACAGTAAGGCTTTCTTCTG PRIMER_INTERNAL_1_SEQUENCE=ATAAACAGCAGGCTCCAGGC PRIMER_LEFT_1=50,24 PRIMER_RIGHT_1=126,25 PRIMER_INTERNAL_1=78,20 PRIMER_LEFT_1_TM=59.919 PRIMER_RIGHT_1_TM=57.559 PRIMER_INTERNAL_1_TM=61.664 PRIMER_LEFT_1_GC_PERCENT=41.667 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=6.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=11.565640 PRIMER_LEFT_2_PENALTY=5.632647 PRIMER_RIGHT_2_PENALTY=5.932992 PRIMER_INTERNAL_2_PENALTY=1.664117 PRIMER_LEFT_2_SEQUENCE=ACAGCATGAAGGAATAATACTGTGG PRIMER_RIGHT_2_SEQUENCE=TACACAGTAAGGCTTTCTTCTGATG PRIMER_INTERNAL_2_SEQUENCE=ATAAACAGCAGGCTCCAGGC PRIMER_LEFT_2=49,25 PRIMER_RIGHT_2=123,25 PRIMER_INTERNAL_2=78,20 PRIMER_LEFT_2_TM=60.633 PRIMER_RIGHT_2_TM=59.067 PRIMER_INTERNAL_2_TM=61.664 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=8.00 PRIMER_PAIR_2_COMPL_END=7.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=11.706451 PRIMER_LEFT_3_PENALTY=4.081412 PRIMER_RIGHT_3_PENALTY=7.625039 PRIMER_INTERNAL_3_PENALTY=1.664117 PRIMER_LEFT_3_SEQUENCE=CAGCATGAAGGAATAATACTGTGG PRIMER_RIGHT_3_SEQUENCE=GTACACAGTAAGGCTTTCTTCTGAT PRIMER_INTERNAL_3_SEQUENCE=ATAAACAGCAGGCTCCAGGC PRIMER_LEFT_3=50,24 PRIMER_RIGHT_3=124,25 PRIMER_INTERNAL_3=78,20 PRIMER_LEFT_3_TM=59.919 PRIMER_RIGHT_3_TM=57.375 PRIMER_INTERNAL_3_TM=61.664 PRIMER_LEFT_3_GC_PERCENT=41.667 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=6.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=11.963614 PRIMER_LEFT_4_PENALTY=4.917956 PRIMER_RIGHT_4_PENALTY=7.045658 PRIMER_INTERNAL_4_PENALTY=1.664117 PRIMER_LEFT_4_SEQUENCE=AGCATGAAGGAATAATACTGTGGA PRIMER_RIGHT_4_SEQUENCE=AGTACACAGTAAGGCTTTCTTCTGA PRIMER_INTERNAL_4_SEQUENCE=ATAAACAGCAGGCTCCAGGC PRIMER_LEFT_4=51,24 PRIMER_RIGHT_4=125,25 PRIMER_INTERNAL_4=78,20 PRIMER_LEFT_4_TM=59.082 PRIMER_RIGHT_4_TM=57.954 PRIMER_INTERNAL_4_TM=61.664 PRIMER_LEFT_4_GC_PERCENT=37.500 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=6.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH343 SEQUENCE_TEMPLATE=GTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTATCTTGTNTGCTCCTTACTGTACAGGTGGCTGACAAAGAGAAGGGAAGATGGAGCCGCCATCTTGAACATGATTGACGCAGGGTNCCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCANCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGC SEQUENCE_INCLUDED_REGION=33,82 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH345 SEQUENCE_TEMPLATE=TTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTTCATAACTAGTNAGGGCCTTCATTCTATCTGAATAGATTNTAGCAAACCATCTATTCCATGCAGATAAATTCAGTCAATTAGGGTCCGNGCTCGAATTCGTAATCATGGTCATAGCTNTTTCCTGTGTGAAATNGTTATCCGCTCACANTTCCACACAACATACGCGCCGGAAGCATAANGTGTAAAGCCTGGGNTGCCTAATGAGNGTGCTAACTCACA SEQUENCE_INCLUDED_REGION=32,83 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH346 SEQUENCE_TEMPLATE=GTTCATGCTCAGGTCGACTCTAGAGGATCCCCTCATTGTGTTATGGTCAAAAAAAAAGTTTTTCAGGGGCAAATATGTGCTTCATTTATGGTAAATTTGTGTTCTCCAACTTTATTTACTTAATGTATTTCTNGTTCTTCTGCATATACAATGNNNTATCATTTCAAGGCTGCTTTGTAGTGTAATCTCTATGCANACATGTTAAGTAAAGCANATTAAGATGCACCATGTATTGTGCAGTCAGTGCAAATAGCCTAGGTGACAATCTGNTGANTGGNTAGATGTCTTCCACCTTGGGCCAACTTCACATANTTGTAAGGGAAGACA SEQUENCE_INCLUDED_REGION=32,295 P3_COMMENT=CL is AAAGTTTTTCAGGGGCAAAT P3_COMMENT=CR is AGGCTATTTGCACTGACTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.894088 PRIMER_LEFT_0_PENALTY=1.010701 PRIMER_RIGHT_0_PENALTY=0.883387 PRIMER_INTERNAL_0_PENALTY=2.095538 PRIMER_LEFT_0_SEQUENCE=GATGCACCATGTATTGTGCAG PRIMER_RIGHT_0_SEQUENCE=TATGTGAAGTTGGCCCAAGG PRIMER_INTERNAL_0_SEQUENCE=TCAGTGCAAATAGCCTAGGTGA PRIMER_LEFT_0=219,21 PRIMER_RIGHT_0=310,20 PRIMER_INTERNAL_0=240,22 PRIMER_LEFT_0_TM=60.011 PRIMER_RIGHT_0_TM=60.883 PRIMER_INTERNAL_0_TM=59.904 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=10.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=8.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=92 PRIMER_PAIR_1_PENALTY=2.310635 PRIMER_LEFT_1_PENALTY=1.427248 PRIMER_RIGHT_1_PENALTY=0.883387 PRIMER_INTERNAL_1_PENALTY=2.548500 PRIMER_LEFT_1_SEQUENCE=TGCACCATGTATTGTGCAGT PRIMER_RIGHT_1_SEQUENCE=TATGTGAAGTTGGCCCAAGG PRIMER_INTERNAL_1_SEQUENCE=TGCAAATAGCCTAGGTGACAA PRIMER_LEFT_1=221,20 PRIMER_RIGHT_1=310,20 PRIMER_INTERNAL_1=244,21 PRIMER_LEFT_1_TM=58.573 PRIMER_RIGHT_1_TM=60.883 PRIMER_INTERNAL_1_TM=58.451 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=10.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=90 PRIMER_PAIR_2_PENALTY=2.440107 PRIMER_LEFT_2_PENALTY=1.010701 PRIMER_RIGHT_2_PENALTY=1.429406 PRIMER_INTERNAL_2_PENALTY=2.095538 PRIMER_LEFT_2_SEQUENCE=GATGCACCATGTATTGTGCAG PRIMER_RIGHT_2_SEQUENCE=GCCCAAGGTGGAAGACATCT PRIMER_INTERNAL_2_SEQUENCE=TCAGTGCAAATAGCCTAGGTGA PRIMER_LEFT_2=219,21 PRIMER_RIGHT_2=298,20 PRIMER_INTERNAL_2=240,22 PRIMER_LEFT_2_TM=60.011 PRIMER_RIGHT_2_TM=61.429 PRIMER_INTERNAL_2_TM=59.904 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=10.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=8.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=2.478967 PRIMER_LEFT_3_PENALTY=1.010701 PRIMER_RIGHT_3_PENALTY=1.468266 PRIMER_INTERNAL_3_PENALTY=2.095538 PRIMER_LEFT_3_SEQUENCE=GATGCACCATGTATTGTGCAG PRIMER_RIGHT_3_SEQUENCE=GCCCAAGGTGGAAGACATC PRIMER_INTERNAL_3_SEQUENCE=TCAGTGCAAATAGCCTAGGTGA PRIMER_LEFT_3=219,21 PRIMER_RIGHT_3=298,19 PRIMER_INTERNAL_3=240,22 PRIMER_LEFT_3_TM=60.011 PRIMER_RIGHT_3_TM=60.468 PRIMER_INTERNAL_3_TM=59.904 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=57.895 PRIMER_INTERNAL_3_GC_PERCENT=45.455 PRIMER_LEFT_3_SELF_ANY=10.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=8.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=6.3000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=2.483650 PRIMER_LEFT_4_PENALTY=1.600264 PRIMER_RIGHT_4_PENALTY=0.883387 PRIMER_INTERNAL_4_PENALTY=2.548500 PRIMER_LEFT_4_SEQUENCE=GCACCATGTATTGTGCAGTCA PRIMER_RIGHT_4_SEQUENCE=TATGTGAAGTTGGCCCAAGG PRIMER_INTERNAL_4_SEQUENCE=TGCAAATAGCCTAGGTGACAA PRIMER_LEFT_4=222,21 PRIMER_RIGHT_4=310,20 PRIMER_INTERNAL_4=244,21 PRIMER_LEFT_4_TM=60.600 PRIMER_RIGHT_4_TM=60.883 PRIMER_INTERNAL_4_TM=58.451 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH347 SEQUENCE_TEMPLATE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTCTATCTGTNNATCTTGCCTCTGAAGTTTTGACGNCTTATGGAGGTACTTGTCTTAAGTCTAGATTTGGGGTNTTCAGAAAATTGCTGTTTTTCTTTTCTGAAGCAGGCTACATGAATGATTGGAGTAGATAAAAAATCACTNTCTGCACAATNTTTTTTGTAAATTAATTAATGTATGGAGCCATTCTAATGATAGATAGGGAAATTATTTCCACTGTAACTTGAAGAACAAAAGCTGCCGTATTTTAAGTTAAAGAGGTCTAAAATT SEQUENCE_INCLUDED_REGION=31,270 P3_COMMENT=CL is TCTTGCCTCTGAAGTTTTGA P3_COMMENT=CR is GACCTCTTTAACTTAAAATACGGC P3_COMMENT=CM is ACTTGTCTTAAGTCTAGATTTGGGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.200640 PRIMER_LEFT_0_PENALTY=0.953367 PRIMER_RIGHT_0_PENALTY=2.247273 PRIMER_INTERNAL_0_PENALTY=6.343049 PRIMER_LEFT_0_SEQUENCE=TTGCCTCTGAAGTTTTGACG PRIMER_RIGHT_0_SEQUENCE=GCCTGCTTCAGAAAAGAAAAA PRIMER_INTERNAL_0_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG PRIMER_LEFT_0=46,20 PRIMER_RIGHT_0=140,21 PRIMER_INTERNAL_0=79,24 PRIMER_LEFT_0_TM=59.047 PRIMER_RIGHT_0_TM=58.753 PRIMER_INTERNAL_0_TM=57.657 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=95 PRIMER_PAIR_1_PENALTY=3.284990 PRIMER_LEFT_1_PENALTY=1.037717 PRIMER_RIGHT_1_PENALTY=2.247273 PRIMER_INTERNAL_1_PENALTY=6.343049 PRIMER_LEFT_1_SEQUENCE=CTTGCCTCTGAAGTTTTGACG PRIMER_RIGHT_1_SEQUENCE=GCCTGCTTCAGAAAAGAAAAA PRIMER_INTERNAL_1_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG PRIMER_LEFT_1=45,21 PRIMER_RIGHT_1=140,21 PRIMER_INTERNAL_1=79,24 PRIMER_LEFT_1_TM=60.038 PRIMER_RIGHT_1_TM=58.753 PRIMER_INTERNAL_1_TM=57.657 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=38.095 PRIMER_INTERNAL_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.4000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=3.286596 PRIMER_LEFT_2_PENALTY=0.953367 PRIMER_RIGHT_2_PENALTY=2.333229 PRIMER_INTERNAL_2_PENALTY=6.343049 PRIMER_LEFT_2_SEQUENCE=TTGCCTCTGAAGTTTTGACG PRIMER_RIGHT_2_SEQUENCE=AGCCTGCTTCAGAAAAGAAAAA PRIMER_INTERNAL_2_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG PRIMER_LEFT_2=46,20 PRIMER_RIGHT_2=141,22 PRIMER_INTERNAL_2=79,24 PRIMER_LEFT_2_TM=59.047 PRIMER_RIGHT_2_TM=59.667 PRIMER_INTERNAL_2_TM=57.657 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=36.364 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=3.370947 PRIMER_LEFT_3_PENALTY=1.037717 PRIMER_RIGHT_3_PENALTY=2.333229 PRIMER_INTERNAL_3_PENALTY=6.343049 PRIMER_LEFT_3_SEQUENCE=CTTGCCTCTGAAGTTTTGACG PRIMER_RIGHT_3_SEQUENCE=AGCCTGCTTCAGAAAAGAAAAA PRIMER_INTERNAL_3_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG PRIMER_LEFT_3=45,21 PRIMER_RIGHT_3=141,22 PRIMER_INTERNAL_3=79,24 PRIMER_LEFT_3_TM=60.038 PRIMER_RIGHT_3_TM=59.667 PRIMER_INTERNAL_3_TM=57.657 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=36.364 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.4000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=3.409191 PRIMER_LEFT_4_PENALTY=0.953367 PRIMER_RIGHT_4_PENALTY=2.455824 PRIMER_INTERNAL_4_PENALTY=6.343049 PRIMER_LEFT_4_SEQUENCE=TTGCCTCTGAAGTTTTGACG PRIMER_RIGHT_4_SEQUENCE=GCCTGCTTCAGAAAAGAAAAAC PRIMER_INTERNAL_4_SEQUENCE=CTTGTCTTAAGTCTAGATTTGGGG PRIMER_LEFT_4=46,20 PRIMER_RIGHT_4=140,22 PRIMER_INTERNAL_4=79,24 PRIMER_LEFT_4_TM=59.047 PRIMER_RIGHT_4_TM=59.544 PRIMER_INTERNAL_4_TM=57.657 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=40.909 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=95 = SEQUENCE_ID=MH348 SEQUENCE_TEMPLATE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTCACACTGATACATCCAATTCAAATTTAACACCATTGGGTATATTATAATTTTNGCTCTATCCATAGTTCTAACCCTCTTCTCTGACAGTGAGACACCTGCCTTCTATTGTCCTTGACGTATTAACGTATTCGATCAGTCACCCATCTGGAACCAAGGTTTCATTTCTGCTGACCCCTCCCTCCTCACCCTACTTGGGCTCTGACTTCCTTTCCTGGGCTGAACCTTCTCTGTGTGGCTGTCCGCCTTCCTCTGCTTGGGCTCCAATACCCTACATGGGTGTCTGCTCATCCTGCGCAGAGGGGTAC SEQUENCE_INCLUDED_REGION=31,308 P3_COMMENT=CL is TTCAAATTTAACACCATTGGG P3_COMMENT=CR is CACCCATGTAGGGTATTGGA P3_COMMENT=CM is GCTCTATCCATAGTTCTAACCCTCT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.302917 PRIMER_LEFT_0_PENALTY=0.139259 PRIMER_RIGHT_0_PENALTY=0.163658 PRIMER_INTERNAL_0_PENALTY=0.550349 PRIMER_LEFT_0_SEQUENCE=CTCACCCTACTTGGGCTCTG PRIMER_RIGHT_0_SEQUENCE=GTATTGGAGCCCAAGCAGAG PRIMER_INTERNAL_0_SEQUENCE=CTGGGCTGAACCTTCTCTGT PRIMER_LEFT_0=215,20 PRIMER_RIGHT_0=300,20 PRIMER_INTERNAL_0=245,20 PRIMER_LEFT_0_TM=59.861 PRIMER_RIGHT_0_TM=59.836 PRIMER_INTERNAL_0_TM=59.450 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=10.00 PRIMER_PAIR_0_COMPL_END=8.00 PRIMER_PAIR_0_PRODUCT_SIZE=86 PRIMER_PAIR_1_PENALTY=0.442505 PRIMER_LEFT_1_PENALTY=0.278847 PRIMER_RIGHT_1_PENALTY=0.163658 PRIMER_INTERNAL_1_PENALTY=0.550349 PRIMER_LEFT_1_SEQUENCE=CACCCTACTTGGGCTCTGAC PRIMER_RIGHT_1_SEQUENCE=GTATTGGAGCCCAAGCAGAG PRIMER_INTERNAL_1_SEQUENCE=CTGGGCTGAACCTTCTCTGT PRIMER_LEFT_1=217,20 PRIMER_RIGHT_1=300,20 PRIMER_INTERNAL_1=245,20 PRIMER_LEFT_1_TM=59.721 PRIMER_RIGHT_1_TM=59.836 PRIMER_INTERNAL_1_TM=59.450 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=10.00 PRIMER_PAIR_1_COMPL_END=8.00 PRIMER_PAIR_1_PRODUCT_SIZE=84 PRIMER_PAIR_2_PENALTY=0.461882 PRIMER_LEFT_2_PENALTY=0.139259 PRIMER_RIGHT_2_PENALTY=0.322623 PRIMER_INTERNAL_2_PENALTY=0.550349 PRIMER_LEFT_2_SEQUENCE=CTCACCCTACTTGGGCTCTG PRIMER_RIGHT_2_SEQUENCE=TGTAGGGTATTGGAGCCCAA PRIMER_INTERNAL_2_SEQUENCE=CTGGGCTGAACCTTCTCTGT PRIMER_LEFT_2=215,20 PRIMER_RIGHT_2=306,20 PRIMER_INTERNAL_2=245,20 PRIMER_LEFT_2_TM=59.861 PRIMER_RIGHT_2_TM=60.323 PRIMER_INTERNAL_2_TM=59.450 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=8.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=92 PRIMER_PAIR_3_PENALTY=0.481624 PRIMER_LEFT_3_PENALTY=0.182682 PRIMER_RIGHT_3_PENALTY=0.298942 PRIMER_INTERNAL_3_PENALTY=0.163658 PRIMER_LEFT_3_SEQUENCE=GGGCTCTGACTTCCTTTCCT PRIMER_RIGHT_3_SEQUENCE=TGAGCAGACACCCATGTAGG PRIMER_INTERNAL_3_SEQUENCE=CTCTGCTTGGGCTCCAATAC PRIMER_LEFT_3=227,20 PRIMER_RIGHT_3=320,20 PRIMER_INTERNAL_3=281,20 PRIMER_LEFT_3_TM=59.817 PRIMER_RIGHT_3_TM=59.701 PRIMER_INTERNAL_3_TM=59.836 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=0.505306 PRIMER_LEFT_4_PENALTY=0.182682 PRIMER_RIGHT_4_PENALTY=0.322623 PRIMER_INTERNAL_4_PENALTY=0.695412 PRIMER_LEFT_4_SEQUENCE=GGGCTCTGACTTCCTTTCCT PRIMER_RIGHT_4_SEQUENCE=TGTAGGGTATTGGAGCCCAA PRIMER_INTERNAL_4_SEQUENCE=GGGCTGAACCTTCTCTGTGT PRIMER_LEFT_4=227,20 PRIMER_RIGHT_4=306,20 PRIMER_INTERNAL_4=247,20 PRIMER_LEFT_4_TM=59.817 PRIMER_RIGHT_4_TM=60.323 PRIMER_INTERNAL_4_TM=59.305 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=10.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH354 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCCTGAATTTAAAAGTNGCCAGTTATTCAGAACCTTGAAAGCACCGTGCAGACCAAGCCAAGGCGCATCCGGTGTGTGCGTCCTCTCCGGTTGGTGGTCTCACTGAGGATGAGGCTGGGGTTTGGATCTTGTGGAGGCAGAGCTGTGTCCTGAGCACAAGAAGGCATGGGATGGGAAAGGCCATTTTCAGCAGCATTTGATGGCATCGAGGTTAGCCAGGCCAGACGTAGAGGGCACTCCCTTTGGGGTCCCCCTTGTTACTGGGGCCCTCT SEQUENCE_INCLUDED_REGION=36,272 P3_COMMENT=CL is CAGTTATTCAGAACCTTGAAAGC P3_COMMENT=CR is AGGGCCCCAGTAACAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.289133 PRIMER_LEFT_0_PENALTY=0.187005 PRIMER_RIGHT_0_PENALTY=0.102129 PRIMER_INTERNAL_0_PENALTY=0.190632 PRIMER_LEFT_0_SEQUENCE=GCTGTGTCCTGAGCACAAGA PRIMER_RIGHT_0_SEQUENCE=CTACGTCTGGCCTGGCTAAC PRIMER_INTERNAL_0_SEQUENCE=TTTTCAGCAGCATTTGATGG PRIMER_LEFT_0=177,20 PRIMER_RIGHT_0=265,20 PRIMER_INTERNAL_0=219,20 PRIMER_LEFT_0_TM=60.187 PRIMER_RIGHT_0_TM=59.898 PRIMER_INTERNAL_0_TM=59.809 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=5.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=89 PRIMER_PAIR_1_PENALTY=0.289133 PRIMER_LEFT_1_PENALTY=0.187005 PRIMER_RIGHT_1_PENALTY=0.102129 PRIMER_INTERNAL_1_PENALTY=0.190632 PRIMER_LEFT_1_SEQUENCE=GAGCTGTGTCCTGAGCACAA PRIMER_RIGHT_1_SEQUENCE=CTACGTCTGGCCTGGCTAAC PRIMER_INTERNAL_1_SEQUENCE=TTTTCAGCAGCATTTGATGG PRIMER_LEFT_1=175,20 PRIMER_RIGHT_1=265,20 PRIMER_INTERNAL_1=219,20 PRIMER_LEFT_1_TM=60.187 PRIMER_RIGHT_1_TM=59.898 PRIMER_INTERNAL_1_TM=59.809 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=5.7000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=0.338502 PRIMER_LEFT_2_PENALTY=0.236374 PRIMER_RIGHT_2_PENALTY=0.102129 PRIMER_INTERNAL_2_PENALTY=0.190632 PRIMER_LEFT_2_SEQUENCE=AGAGCTGTGTCCTGAGCACA PRIMER_RIGHT_2_SEQUENCE=CTACGTCTGGCCTGGCTAAC PRIMER_INTERNAL_2_SEQUENCE=TTTTCAGCAGCATTTGATGG PRIMER_LEFT_2=174,20 PRIMER_RIGHT_2=265,20 PRIMER_INTERNAL_2=219,20 PRIMER_LEFT_2_TM=59.764 PRIMER_RIGHT_2_TM=59.898 PRIMER_INTERNAL_2_TM=59.809 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=7.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=5.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=92 PRIMER_PAIR_3_PENALTY=0.338502 PRIMER_LEFT_3_PENALTY=0.236374 PRIMER_RIGHT_3_PENALTY=0.102129 PRIMER_INTERNAL_3_PENALTY=0.190632 PRIMER_LEFT_3_SEQUENCE=CAGAGCTGTGTCCTGAGCAC PRIMER_RIGHT_3_SEQUENCE=CTACGTCTGGCCTGGCTAAC PRIMER_INTERNAL_3_SEQUENCE=TTTTCAGCAGCATTTGATGG PRIMER_LEFT_3=173,20 PRIMER_RIGHT_3=265,20 PRIMER_INTERNAL_3=219,20 PRIMER_LEFT_3_TM=59.764 PRIMER_RIGHT_3_TM=59.898 PRIMER_INTERNAL_3_TM=59.809 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=5.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=93 PRIMER_PAIR_4_PENALTY=0.491819 PRIMER_LEFT_4_PENALTY=0.290589 PRIMER_RIGHT_4_PENALTY=0.201230 PRIMER_INTERNAL_4_PENALTY=2.261603 PRIMER_LEFT_4_SEQUENCE=AGAACCTTGAAAGCACCGTG PRIMER_RIGHT_4_SEQUENCE=GCCTCATCCTCAGTGAGACC PRIMER_INTERNAL_4_SEQUENCE=GGTGTGTGCGTCCTCTCC PRIMER_LEFT_4=64,20 PRIMER_RIGHT_4=150,20 PRIMER_INTERNAL_4=106,18 PRIMER_LEFT_4_TM=60.291 PRIMER_RIGHT_4_TM=59.799 PRIMER_INTERNAL_4_TM=60.262 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_INTERNAL_4_GC_PERCENT=66.667 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.9000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH356 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAATAATAAGGAATTACTTAGTCAAGATAGTTCTTGATCGGTAAATTTTGTCTCACGTACATAGCAGTATATAAAATTCAATTTTTATTTTAGTTAAACTAAGTNTCAAATGACCACAGTGACGATTCCAGCACTGGATCCTGTATTCCCTGGTATTCTATTAGGTCATATTTCAACTACTGTAAATATGTAAATTCTGTTACACAGAAGATCTGGAAATAACCAAATATTCTTTTAAAAGTCAAAACTGTTAGCACTTCAAACCCATTTACTAAAAACTAGAGT SEQUENCE_INCLUDED_REGION=36,288 P3_COMMENT=CL is TTAGTCAAGATAGTTCTTGATCGG P3_COMMENT=CR is TTTAGTAAATGGGTTTGAAGTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.508092 PRIMER_LEFT_0_PENALTY=0.759154 PRIMER_RIGHT_0_PENALTY=4.748938 PRIMER_INTERNAL_0_PENALTY=6.732607 PRIMER_LEFT_0_SEQUENCE=TTCCAGCACTGGATCCTGTA PRIMER_RIGHT_0_SEQUENCE=TGGTTATTTCCAGATCTTCTGTG PRIMER_INTERNAL_0_SEQUENCE=TTCCCTGGTATTCTATTAGGTCAT PRIMER_LEFT_0=164,20 PRIMER_RIGHT_0=263,23 PRIMER_INTERNAL_0=184,24 PRIMER_LEFT_0_TM=59.241 PRIMER_RIGHT_0_TM=58.251 PRIMER_INTERNAL_0_TM=57.267 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=39.130 PRIMER_INTERNAL_0_GC_PERCENT=37.500 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=5.7000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=5.690360 PRIMER_LEFT_1_PENALTY=0.759154 PRIMER_RIGHT_1_PENALTY=4.931205 PRIMER_INTERNAL_1_PENALTY=6.732607 PRIMER_LEFT_1_SEQUENCE=TTCCAGCACTGGATCCTGTA PRIMER_RIGHT_1_SEQUENCE=TGGTTATTTCCAGATCTTCTGTGT PRIMER_INTERNAL_1_SEQUENCE=TTCCCTGGTATTCTATTAGGTCAT PRIMER_LEFT_1=164,20 PRIMER_RIGHT_1=263,24 PRIMER_INTERNAL_1=184,24 PRIMER_LEFT_1_TM=59.241 PRIMER_RIGHT_1_TM=59.069 PRIMER_INTERNAL_1_TM=57.267 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=37.500 PRIMER_INTERNAL_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=5.7000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=5.895779 PRIMER_LEFT_2_PENALTY=1.435246 PRIMER_RIGHT_2_PENALTY=4.460533 PRIMER_INTERNAL_2_PENALTY=8.821726 PRIMER_LEFT_2_SEQUENCE=CCAGCACTGGATCCTGTATTC PRIMER_RIGHT_2_SEQUENCE=TTGGTTATTTCCAGATCTTCTGTG PRIMER_INTERNAL_2_SEQUENCE=CCTGGTATTCTATTAGGTCATATTTCA PRIMER_LEFT_2=166,21 PRIMER_RIGHT_2=264,24 PRIMER_INTERNAL_2=187,27 PRIMER_LEFT_2_TM=59.565 PRIMER_RIGHT_2_TM=59.539 PRIMER_INTERNAL_2_TM=58.178 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=37.500 PRIMER_INTERNAL_2_GC_PERCENT=33.333 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=5.9000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=5.895779 PRIMER_LEFT_3_PENALTY=1.435246 PRIMER_RIGHT_3_PENALTY=4.460533 PRIMER_INTERNAL_3_PENALTY=8.188024 PRIMER_LEFT_3_SEQUENCE=TCCAGCACTGGATCCTGTATT PRIMER_RIGHT_3_SEQUENCE=TTGGTTATTTCCAGATCTTCTGTG PRIMER_INTERNAL_3_SEQUENCE=CCCTGGTATTCTATTAGGTCATATTTC PRIMER_LEFT_3=165,21 PRIMER_RIGHT_3=264,24 PRIMER_INTERNAL_3=186,27 PRIMER_LEFT_3_TM=59.565 PRIMER_RIGHT_3_TM=59.539 PRIMER_INTERNAL_3_TM=58.812 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=37.500 PRIMER_INTERNAL_3_GC_PERCENT=37.037 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=5.6000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=6.184184 PRIMER_LEFT_4_PENALTY=1.435246 PRIMER_RIGHT_4_PENALTY=4.748938 PRIMER_INTERNAL_4_PENALTY=8.821726 PRIMER_LEFT_4_SEQUENCE=CCAGCACTGGATCCTGTATTC PRIMER_RIGHT_4_SEQUENCE=TGGTTATTTCCAGATCTTCTGTG PRIMER_INTERNAL_4_SEQUENCE=CCTGGTATTCTATTAGGTCATATTTCA PRIMER_LEFT_4=166,21 PRIMER_RIGHT_4=263,23 PRIMER_INTERNAL_4=187,27 PRIMER_LEFT_4_TM=59.565 PRIMER_RIGHT_4_TM=58.251 PRIMER_INTERNAL_4_TM=58.178 PRIMER_LEFT_4_GC_PERCENT=52.381 PRIMER_RIGHT_4_GC_PERCENT=39.130 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=5.9000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH362 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTGGCTTTGGAACTAAGCCTGAAGGTCAAATAGGGTATCCATAGCAGGGGTCTGGGGCACTAGACAGTCTGTTCATTTTCAGGAATATATGGGTCANTTTTGTATGTGTGACCT SEQUENCE_INCLUDED_REGION=33,113 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.681940 PRIMER_LEFT_0_PENALTY=0.377925 PRIMER_RIGHT_0_PENALTY=3.304014 PRIMER_INTERNAL_0_PENALTY=0.326207 PRIMER_LEFT_0_SEQUENCE=TGGCTTTGGAACTAAGCCTG PRIMER_RIGHT_0_SEQUENCE=AATGAACAGACTGTCTAGTGCCC PRIMER_INTERNAL_0_SEQUENCE=AGGGTATCCATAGCAGGGGT PRIMER_LEFT_0=33,20 PRIMER_RIGHT_0=108,23 PRIMER_INTERNAL_0=63,20 PRIMER_LEFT_0_TM=60.378 PRIMER_RIGHT_0_TM=59.696 PRIMER_INTERNAL_0_TM=59.674 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=47.826 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=11.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=3.851522 PRIMER_LEFT_1_PENALTY=0.547508 PRIMER_RIGHT_1_PENALTY=3.304014 PRIMER_INTERNAL_1_PENALTY=0.326207 PRIMER_LEFT_1_SEQUENCE=GGCTTTGGAACTAAGCCTGA PRIMER_RIGHT_1_SEQUENCE=AATGAACAGACTGTCTAGTGCCC PRIMER_INTERNAL_1_SEQUENCE=AGGGTATCCATAGCAGGGGT PRIMER_LEFT_1=34,20 PRIMER_RIGHT_1=108,23 PRIMER_INTERNAL_1=63,20 PRIMER_LEFT_1_TM=59.452 PRIMER_RIGHT_1_TM=59.696 PRIMER_INTERNAL_1_TM=59.674 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=47.826 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=11.2000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=4.042376 PRIMER_LEFT_2_PENALTY=0.377925 PRIMER_RIGHT_2_PENALTY=3.664451 PRIMER_INTERNAL_2_PENALTY=0.326207 PRIMER_LEFT_2_SEQUENCE=TGGCTTTGGAACTAAGCCTG PRIMER_RIGHT_2_SEQUENCE=ATGAACAGACTGTCTAGTGCCC PRIMER_INTERNAL_2_SEQUENCE=AGGGTATCCATAGCAGGGGT PRIMER_LEFT_2=33,20 PRIMER_RIGHT_2=107,22 PRIMER_INTERNAL_2=63,20 PRIMER_LEFT_2_TM=60.378 PRIMER_RIGHT_2_TM=58.336 PRIMER_INTERNAL_2_TM=59.674 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=11.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=4.790071 PRIMER_LEFT_3_PENALTY=0.377925 PRIMER_RIGHT_3_PENALTY=4.412146 PRIMER_INTERNAL_3_PENALTY=0.090155 PRIMER_LEFT_3_SEQUENCE=TGGCTTTGGAACTAAGCCTG PRIMER_RIGHT_3_SEQUENCE=TGACCCATATATTCCTGAAAATGA PRIMER_INTERNAL_3_SEQUENCE=TATCCATAGCAGGGGTCTGG PRIMER_LEFT_3=33,20 PRIMER_RIGHT_3=127,24 PRIMER_INTERNAL_3=67,20 PRIMER_LEFT_3_TM=60.378 PRIMER_RIGHT_3_TM=59.588 PRIMER_INTERNAL_3_TM=59.910 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=95 PRIMER_PAIR_4_PENALTY=4.959654 PRIMER_LEFT_4_PENALTY=0.547508 PRIMER_RIGHT_4_PENALTY=4.412146 PRIMER_INTERNAL_4_PENALTY=0.090155 PRIMER_LEFT_4_SEQUENCE=GGCTTTGGAACTAAGCCTGA PRIMER_RIGHT_4_SEQUENCE=TGACCCATATATTCCTGAAAATGA PRIMER_INTERNAL_4_SEQUENCE=TATCCATAGCAGGGGTCTGG PRIMER_LEFT_4=34,20 PRIMER_RIGHT_4=127,24 PRIMER_INTERNAL_4=67,20 PRIMER_LEFT_4_TM=59.452 PRIMER_RIGHT_4_TM=59.588 PRIMER_INTERNAL_4_TM=59.910 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=33.333 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=94 = SEQUENCE_ID=MH364 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAGGCTTCCTTTCTCTACAAGGAGGAAATTGAAACCATTTGTGAAAGAGCATTTCCTTTTCAGAGTCTTATCCTAGTACCTCCGAGAACTCTGTGAGTCTGTTCATAGACGGGGAGGGAGCAGGAGAAAAGGCTGAGGGCTTTTTAATACCTGGGNCAAAAGAATCTTTCCCTTAAAATAAAAAATGC SEQUENCE_INCLUDED_REGION=36,190 P3_COMMENT=CL is TAGGCTTCCTTTCTCTACAAGG P3_COMMENT=CR is CCCAGGTATTAAAAAGCCCT P3_COMMENT=CM is GGAAATTGAAACCATTTGTGA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.421963 PRIMER_LEFT_0_PENALTY=2.356567 PRIMER_RIGHT_0_PENALTY=0.065396 PRIMER_INTERNAL_0_PENALTY=3.234797 PRIMER_LEFT_0_SEQUENCE=CAAGGAGGAAATTGAAACCA PRIMER_RIGHT_0_SEQUENCE=CCTCCCCGTCTATGAACAGA PRIMER_INTERNAL_0_SEQUENCE=TTGTGAAAGAGCATTTCCTTTTC PRIMER_LEFT_0=55,20 PRIMER_RIGHT_0=154,20 PRIMER_INTERNAL_0=76,23 PRIMER_LEFT_0_TM=57.643 PRIMER_RIGHT_0_TM=60.065 PRIMER_INTERNAL_0_TM=59.765 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=2.660388 PRIMER_LEFT_1_PENALTY=2.594992 PRIMER_RIGHT_1_PENALTY=0.065396 PRIMER_INTERNAL_1_PENALTY=3.349942 PRIMER_LEFT_1_SEQUENCE=GGAGGAAATTGAAACCATTTG PRIMER_RIGHT_1_SEQUENCE=CCTCCCCGTCTATGAACAGA PRIMER_INTERNAL_1_SEQUENCE=TGAAAGAGCATTTCCTTTTCAGA PRIMER_LEFT_1=58,21 PRIMER_RIGHT_1=154,20 PRIMER_INTERNAL_1=79,23 PRIMER_LEFT_1_TM=58.405 PRIMER_RIGHT_1_TM=60.065 PRIMER_INTERNAL_1_TM=60.350 PRIMER_LEFT_1_GC_PERCENT=38.095 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=34.783 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=97 PRIMER_PAIR_2_PENALTY=2.713818 PRIMER_LEFT_2_PENALTY=2.648422 PRIMER_RIGHT_2_PENALTY=0.065396 PRIMER_INTERNAL_2_PENALTY=3.882472 PRIMER_LEFT_2_SEQUENCE=TGAAACCATTTGTGAAAGAGCA PRIMER_RIGHT_2_SEQUENCE=CCTCCCCGTCTATGAACAGA PRIMER_INTERNAL_2_SEQUENCE=TACCTCCGAGAACTCTGTGAG PRIMER_LEFT_2=67,22 PRIMER_RIGHT_2=154,20 PRIMER_INTERNAL_2=114,21 PRIMER_LEFT_2_TM=60.648 PRIMER_RIGHT_2_TM=60.065 PRIMER_INTERNAL_2_TM=57.118 PRIMER_LEFT_2_GC_PERCENT=36.364 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=88 PRIMER_PAIR_3_PENALTY=2.714943 PRIMER_LEFT_3_PENALTY=2.649547 PRIMER_RIGHT_3_PENALTY=0.065396 PRIMER_INTERNAL_3_PENALTY=3.349942 PRIMER_LEFT_3_SEQUENCE=AGGAGGAAATTGAAACCATTTG PRIMER_RIGHT_3_SEQUENCE=CCTCCCCGTCTATGAACAGA PRIMER_INTERNAL_3_SEQUENCE=TGAAAGAGCATTTCCTTTTCAGA PRIMER_LEFT_3=57,22 PRIMER_RIGHT_3=154,20 PRIMER_INTERNAL_3=79,23 PRIMER_LEFT_3_TM=59.350 PRIMER_RIGHT_3_TM=60.065 PRIMER_INTERNAL_3_TM=60.350 PRIMER_LEFT_3_GC_PERCENT=36.364 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=34.783 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=2.714943 PRIMER_LEFT_4_PENALTY=2.649547 PRIMER_RIGHT_4_PENALTY=0.065396 PRIMER_INTERNAL_4_PENALTY=3.349942 PRIMER_LEFT_4_SEQUENCE=CAAGGAGGAAATTGAAACCATT PRIMER_RIGHT_4_SEQUENCE=CCTCCCCGTCTATGAACAGA PRIMER_INTERNAL_4_SEQUENCE=TGAAAGAGCATTTCCTTTTCAGA PRIMER_LEFT_4=55,22 PRIMER_RIGHT_4=154,20 PRIMER_INTERNAL_4=79,23 PRIMER_LEFT_4_TM=59.350 PRIMER_RIGHT_4_TM=60.065 PRIMER_INTERNAL_4_TM=60.350 PRIMER_LEFT_4_GC_PERCENT=36.364 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=34.783 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH367 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTCGGGAGGCTGAGGCAGGAGAATGGCCAGAACCTAGGAGGCGGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTT SEQUENCE_INCLUDED_REGION=36,49 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH373 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTATTCTCTCTCTGTTTCACTCTCACTTCCCCTTTCTCTGCCTTGAATGCCTATNATATATTGGCTTTACTTGGATTTCCTCTTTAAATTGAAGGTGAATATAATGTCTTTACTTTTTGGGTTATTGGCTCTGTAGATGTTCCTCTTCAGTTGCAAGGGACAGAAACCAATCTATTTTTCCTGACAACCTCTGAAATAAAAGTATGGAAAAGTTAAATGGCTTTAATATAAG SEQUENCE_INCLUDED_REGION=34,235 P3_COMMENT=CL is TGTTTCACTCTCACTTCCCC P3_COMMENT=CR is GTTTCTGTCCCTTGCAACTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.757357 PRIMER_LEFT_0_PENALTY=2.390906 PRIMER_RIGHT_0_PENALTY=0.366450 PRIMER_INTERNAL_0_PENALTY=2.244591 PRIMER_LEFT_0_SEQUENCE=TTGGCTTTACTTGGATTTCCTC PRIMER_RIGHT_0_SEQUENCE=CCCTTGCAACTGAAGAGGAA PRIMER_INTERNAL_0_SEQUENCE=TTTTTGGGTTATTGGCTCTG PRIMER_LEFT_0=97,22 PRIMER_RIGHT_0=195,20 PRIMER_INTERNAL_0=150,20 PRIMER_LEFT_0_TM=59.609 PRIMER_RIGHT_0_TM=60.366 PRIMER_INTERNAL_0_TM=57.755 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=6.00 PRIMER_PAIR_0_PRODUCT_SIZE=99 PRIMER_PAIR_1_PENALTY=2.840700 PRIMER_LEFT_1_PENALTY=2.684639 PRIMER_RIGHT_1_PENALTY=0.156061 PRIMER_INTERNAL_1_PENALTY=2.244591 PRIMER_LEFT_1_SEQUENCE=TGGCTTTACTTGGATTTCCTC PRIMER_RIGHT_1_SEQUENCE=GTCCCTTGCAACTGAAGAGG PRIMER_INTERNAL_1_SEQUENCE=TTTTTGGGTTATTGGCTCTG PRIMER_LEFT_1=98,21 PRIMER_RIGHT_1=197,20 PRIMER_INTERNAL_1=150,20 PRIMER_LEFT_1_TM=58.315 PRIMER_RIGHT_1_TM=59.844 PRIMER_INTERNAL_1_TM=57.755 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=2.892165 PRIMER_LEFT_2_PENALTY=2.736104 PRIMER_RIGHT_2_PENALTY=0.156061 PRIMER_INTERNAL_2_PENALTY=2.244591 PRIMER_LEFT_2_SEQUENCE=TGGCTTTACTTGGATTTCCTCT PRIMER_RIGHT_2_SEQUENCE=GTCCCTTGCAACTGAAGAGG PRIMER_INTERNAL_2_SEQUENCE=TTTTTGGGTTATTGGCTCTG PRIMER_LEFT_2=98,22 PRIMER_RIGHT_2=197,20 PRIMER_INTERNAL_2=150,20 PRIMER_LEFT_2_TM=59.264 PRIMER_RIGHT_2_TM=59.844 PRIMER_INTERNAL_2_TM=57.755 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=5.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=3.051089 PRIMER_LEFT_3_PENALTY=2.684639 PRIMER_RIGHT_3_PENALTY=0.366450 PRIMER_INTERNAL_3_PENALTY=2.244591 PRIMER_LEFT_3_SEQUENCE=TGGCTTTACTTGGATTTCCTC PRIMER_RIGHT_3_SEQUENCE=CCCTTGCAACTGAAGAGGAA PRIMER_INTERNAL_3_SEQUENCE=TTTTTGGGTTATTGGCTCTG PRIMER_LEFT_3=98,21 PRIMER_RIGHT_3=195,20 PRIMER_INTERNAL_3=150,20 PRIMER_LEFT_3_TM=58.315 PRIMER_RIGHT_3_TM=60.366 PRIMER_INTERNAL_3_TM=57.755 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=6.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=3.102554 PRIMER_LEFT_4_PENALTY=2.736104 PRIMER_RIGHT_4_PENALTY=0.366450 PRIMER_INTERNAL_4_PENALTY=2.244591 PRIMER_LEFT_4_SEQUENCE=TGGCTTTACTTGGATTTCCTCT PRIMER_RIGHT_4_SEQUENCE=CCCTTGCAACTGAAGAGGAA PRIMER_INTERNAL_4_SEQUENCE=TTTTTGGGTTATTGGCTCTG PRIMER_LEFT_4=98,22 PRIMER_RIGHT_4=195,20 PRIMER_INTERNAL_4=150,20 PRIMER_LEFT_4_TM=59.264 PRIMER_RIGHT_4_TM=60.366 PRIMER_INTERNAL_4_TM=57.755 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=7.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH375 SEQUENCE_TEMPLATE=CATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCTAGCATTTAGACCTGGAAGCCTTTCTGCTACCCAAAGTGCCACGTGTTGGGTTGAAGCAGGCTGCCTCAGGACAACCAGTCACTCGGAGCACTTGCCTCCATATCCAGGCTAACCAGTGTGTTGGTATAAGTGGTTTTCAAGTAGGG SEQUENCE_INCLUDED_REGION=31,151 P3_COMMENT=CL is CTTCTAGCATTTAGACCTGGA P3_COMMENT=CR is CCTACTTGAAAACCACTTATACC P3_COMMENT=CM is GCCTTTCTGCTACCCAAAGT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.590422 PRIMER_LEFT_0_PENALTY=0.248900 PRIMER_RIGHT_0_PENALTY=0.341522 PRIMER_INTERNAL_0_PENALTY=0.723365 PRIMER_LEFT_0_SEQUENCE=TTTCTGCTACCCAAAGTGCC PRIMER_RIGHT_0_SEQUENCE=GATATGGAGGCAAGTGCTCC PRIMER_INTERNAL_0_SEQUENCE=GCCTCAGGACAACCAGTCAC PRIMER_LEFT_0=56,20 PRIMER_RIGHT_0=139,20 PRIMER_INTERNAL_0=98,20 PRIMER_LEFT_0_TM=60.249 PRIMER_RIGHT_0_TM=59.658 PRIMER_INTERNAL_0_TM=60.723 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=84 PRIMER_PAIR_1_PENALTY=0.590422 PRIMER_LEFT_1_PENALTY=0.248900 PRIMER_RIGHT_1_PENALTY=0.341522 PRIMER_INTERNAL_1_PENALTY=0.135808 PRIMER_LEFT_1_SEQUENCE=TTTCTGCTACCCAAAGTGCC PRIMER_RIGHT_1_SEQUENCE=GGATATGGAGGCAAGTGCTC PRIMER_INTERNAL_1_SEQUENCE=TCAGGACAACCAGTCACTCG PRIMER_LEFT_1=56,20 PRIMER_RIGHT_1=140,20 PRIMER_INTERNAL_1=101,20 PRIMER_LEFT_1_TM=60.249 PRIMER_RIGHT_1_TM=59.658 PRIMER_INTERNAL_1_TM=59.864 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=85 PRIMER_PAIR_2_PENALTY=0.640168 PRIMER_LEFT_2_PENALTY=0.547508 PRIMER_RIGHT_2_PENALTY=0.092660 PRIMER_INTERNAL_2_PENALTY=1.264463 PRIMER_LEFT_2_SEQUENCE=GAAGCCTTTCTGCTACCCAA PRIMER_RIGHT_2_SEQUENCE=AGTGCTCCGAGTGACTGGTT PRIMER_INTERNAL_2_SEQUENCE=ACGTGTTGGGTTGAAGCAG PRIMER_LEFT_2=50,20 PRIMER_RIGHT_2=127,20 PRIMER_INTERNAL_2=76,19 PRIMER_LEFT_2_TM=59.452 PRIMER_RIGHT_2_TM=59.907 PRIMER_INTERNAL_2_TM=59.736 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=52.632 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=10.0000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=78 PRIMER_PAIR_3_PENALTY=0.640168 PRIMER_LEFT_3_PENALTY=0.547508 PRIMER_RIGHT_3_PENALTY=0.092660 PRIMER_INTERNAL_3_PENALTY=1.264463 PRIMER_LEFT_3_SEQUENCE=GAAGCCTTTCTGCTACCCAA PRIMER_RIGHT_3_SEQUENCE=AAGTGCTCCGAGTGACTGGT PRIMER_INTERNAL_3_SEQUENCE=ACGTGTTGGGTTGAAGCAG PRIMER_LEFT_3=50,20 PRIMER_RIGHT_3=128,20 PRIMER_INTERNAL_3=76,19 PRIMER_LEFT_3_TM=59.452 PRIMER_RIGHT_3_TM=59.907 PRIMER_INTERNAL_3_TM=59.736 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=52.632 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=10.0000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=0.722452 PRIMER_LEFT_4_PENALTY=0.248900 PRIMER_RIGHT_4_PENALTY=0.473552 PRIMER_INTERNAL_4_PENALTY=0.135808 PRIMER_LEFT_4_SEQUENCE=TTTCTGCTACCCAAAGTGCC PRIMER_RIGHT_4_SEQUENCE=CCTGGATATGGAGGCAAGTG PRIMER_INTERNAL_4_SEQUENCE=TCAGGACAACCAGTCACTCG PRIMER_LEFT_4=56,20 PRIMER_RIGHT_4=143,20 PRIMER_INTERNAL_4=101,20 PRIMER_LEFT_4_TM=60.249 PRIMER_RIGHT_4_TM=60.474 PRIMER_INTERNAL_4_TM=59.864 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=88 = SEQUENCE_ID=MH378 SEQUENCE_TEMPLATE=CATCTGGGATTTTAGTTTTTACTATTAATATTTGTGGGAACATTTCCGTCATTAAAAACTCCACATCATCATACCATATGGTGTAATAATATCCGCTTGTNTGGGCATACCATGACTTAACTGCTCTCCTATTGTTACATGTTTAGAATGTTTCCATTTTTTCACAATTAAAAATGGTGCCATCTGTGCTCATATCCATGATTATTTCCTTAAGTAAGTTCCAGAAGTGGAATTGTTGTATCAAGGGATTTGACAAATTCTAAAGCTTTCG SEQUENCE_INCLUDED_REGION=0,271 P3_COMMENT=CL is CATCTGGGATTTTAGTTTTTACTAT P3_COMMENT=CR is AATCCCTTGATACAACAATTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.464599 PRIMER_LEFT_0_PENALTY=0.347318 PRIMER_RIGHT_0_PENALTY=2.117281 PRIMER_INTERNAL_0_PENALTY=3.162827 PRIMER_LEFT_0_SEQUENCE=GTGGGAACATTTCCGTCATT PRIMER_RIGHT_0_SEQUENCE=CAGTTAAGTCATGGTATGCCCA PRIMER_INTERNAL_0_SEQUENCE=CCACATCATCATACCATATGGTG PRIMER_LEFT_0=33,20 PRIMER_RIGHT_0=122,22 PRIMER_INTERNAL_0=60,23 PRIMER_LEFT_0_TM=59.653 PRIMER_RIGHT_0_TM=59.883 PRIMER_INTERNAL_0_TM=59.837 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_INTERNAL_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=11.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=90 PRIMER_PAIR_1_PENALTY=2.481949 PRIMER_LEFT_1_PENALTY=2.117281 PRIMER_RIGHT_1_PENALTY=0.364668 PRIMER_INTERNAL_1_PENALTY=5.273133 PRIMER_LEFT_1_SEQUENCE=TGGGCATACCATGACTTAACTG PRIMER_RIGHT_1_SEQUENCE=GGATATGAGCACAGATGGCA PRIMER_INTERNAL_1_SEQUENCE=AGAATGTTTCCATTTTTTCACAA PRIMER_LEFT_1=101,22 PRIMER_RIGHT_1=196,20 PRIMER_INTERNAL_1=144,23 PRIMER_LEFT_1_TM=59.883 PRIMER_RIGHT_1_TM=59.635 PRIMER_INTERNAL_1_TM=57.727 PRIMER_LEFT_1_GC_PERCENT=45.455 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=26.087 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=2.481949 PRIMER_LEFT_2_PENALTY=2.117281 PRIMER_RIGHT_2_PENALTY=0.364668 PRIMER_INTERNAL_2_PENALTY=5.273133 PRIMER_LEFT_2_SEQUENCE=TGGGCATACCATGACTTAACTG PRIMER_RIGHT_2_SEQUENCE=TGGATATGAGCACAGATGGC PRIMER_INTERNAL_2_SEQUENCE=AGAATGTTTCCATTTTTTCACAA PRIMER_LEFT_2=101,22 PRIMER_RIGHT_2=197,20 PRIMER_INTERNAL_2=144,23 PRIMER_LEFT_2_TM=59.883 PRIMER_RIGHT_2_TM=59.635 PRIMER_INTERNAL_2_TM=57.727 PRIMER_LEFT_2_GC_PERCENT=45.455 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=26.087 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=9.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=97 PRIMER_PAIR_3_PENALTY=2.643199 PRIMER_LEFT_3_PENALTY=2.117281 PRIMER_RIGHT_3_PENALTY=0.525918 PRIMER_INTERNAL_3_PENALTY=5.273133 PRIMER_LEFT_3_SEQUENCE=TGGGCATACCATGACTTAACTG PRIMER_RIGHT_3_SEQUENCE=AGCACAGATGGCACCATTTT PRIMER_INTERNAL_3_SEQUENCE=AGAATGTTTCCATTTTTTCACAA PRIMER_LEFT_3=101,22 PRIMER_RIGHT_3=189,20 PRIMER_INTERNAL_3=144,23 PRIMER_LEFT_3_TM=59.883 PRIMER_RIGHT_3_TM=60.526 PRIMER_INTERNAL_3_TM=57.727 PRIMER_LEFT_3_GC_PERCENT=45.455 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=26.087 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=89 PRIMER_PAIR_4_PENALTY=2.851445 PRIMER_LEFT_4_PENALTY=0.734164 PRIMER_RIGHT_4_PENALTY=2.117281 PRIMER_INTERNAL_4_PENALTY=3.162827 PRIMER_LEFT_4_SEQUENCE=TTTGTGGGAACATTTCCGTC PRIMER_RIGHT_4_SEQUENCE=CAGTTAAGTCATGGTATGCCCA PRIMER_INTERNAL_4_SEQUENCE=CCACATCATCATACCATATGGTG PRIMER_LEFT_4=30,20 PRIMER_RIGHT_4=122,22 PRIMER_INTERNAL_4=60,23 PRIMER_LEFT_4_TM=60.734 PRIMER_RIGHT_4_TM=59.883 PRIMER_INTERNAL_4_TM=59.837 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.455 PRIMER_INTERNAL_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=10.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=8.00 PRIMER_LEFT_4_END_STABILITY=9.6000 PRIMER_RIGHT_4_END_STABILITY=11.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=93 = SEQUENCE_ID=MH379 SEQUENCE_TEMPLATE=GTTTTGCGGGTTAGAGAGCGGGGCGTGGGGGCGGGGAGGGAGAGTAACAATTTTGGAAGTCCCTGCGTGGTATCTGGAAAGTTAACGACGCTTCAGGTTCTAGGTTGAGCCCAATGACCGTCTTGCCNNGGCAGGGTACCNAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTNAAATTGTTATCCGCTCACAATTCCNCACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT SEQUENCE_INCLUDED_REGION=0,133 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.565856 PRIMER_LEFT_0_PENALTY=0.319419 PRIMER_RIGHT_0_PENALTY=0.246438 PRIMER_INTERNAL_0_PENALTY=0.133741 PRIMER_LEFT_0_SEQUENCE=CGGGGAGGGAGAGTAACAAT PRIMER_RIGHT_0_SEQUENCE=TGGGCTCAACCTAGAACCTG PRIMER_INTERNAL_0_SEQUENCE=AAGTCCCTGCGTGGTATCTG PRIMER_LEFT_0=31,20 PRIMER_RIGHT_0=112,20 PRIMER_INTERNAL_0=56,20 PRIMER_LEFT_0_TM=60.319 PRIMER_RIGHT_0_TM=60.246 PRIMER_INTERNAL_0_TM=60.134 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=0.769248 PRIMER_LEFT_1_PENALTY=0.319419 PRIMER_RIGHT_1_PENALTY=0.449829 PRIMER_INTERNAL_1_PENALTY=0.133741 PRIMER_LEFT_1_SEQUENCE=CGGGGAGGGAGAGTAACAAT PRIMER_RIGHT_1_SEQUENCE=GGTCATTGGGCTCAACCTAG PRIMER_INTERNAL_1_SEQUENCE=AAGTCCCTGCGTGGTATCTG PRIMER_LEFT_1=31,20 PRIMER_RIGHT_1=118,20 PRIMER_INTERNAL_1=56,20 PRIMER_LEFT_1_TM=60.319 PRIMER_RIGHT_1_TM=59.550 PRIMER_INTERNAL_1_TM=60.134 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=7.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=88 PRIMER_PAIR_2_PENALTY=1.035545 PRIMER_LEFT_2_PENALTY=0.319419 PRIMER_RIGHT_2_PENALTY=0.716126 PRIMER_INTERNAL_2_PENALTY=0.133741 PRIMER_LEFT_2_SEQUENCE=CGGGGAGGGAGAGTAACAAT PRIMER_RIGHT_2_SEQUENCE=GGGCTCAACCTAGAACCTGA PRIMER_INTERNAL_2_SEQUENCE=AAGTCCCTGCGTGGTATCTG PRIMER_LEFT_2=31,20 PRIMER_RIGHT_2=111,20 PRIMER_INTERNAL_2=56,20 PRIMER_LEFT_2_TM=60.319 PRIMER_RIGHT_2_TM=59.284 PRIMER_INTERNAL_2_TM=60.134 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=1.296602 PRIMER_LEFT_3_PENALTY=1.050165 PRIMER_RIGHT_3_PENALTY=0.246438 PRIMER_INTERNAL_3_PENALTY=0.133741 PRIMER_LEFT_3_SEQUENCE=CGGGGAGGGAGAGTAACAA PRIMER_RIGHT_3_SEQUENCE=TGGGCTCAACCTAGAACCTG PRIMER_INTERNAL_3_SEQUENCE=AAGTCCCTGCGTGGTATCTG PRIMER_LEFT_3=31,19 PRIMER_RIGHT_3=112,20 PRIMER_INTERNAL_3=56,20 PRIMER_LEFT_3_TM=60.050 PRIMER_RIGHT_3_TM=60.246 PRIMER_INTERNAL_3_TM=60.134 PRIMER_LEFT_3_GC_PERCENT=57.895 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=82 PRIMER_PAIR_4_PENALTY=1.499994 PRIMER_LEFT_4_PENALTY=1.050165 PRIMER_RIGHT_4_PENALTY=0.449829 PRIMER_INTERNAL_4_PENALTY=0.133741 PRIMER_LEFT_4_SEQUENCE=CGGGGAGGGAGAGTAACAA PRIMER_RIGHT_4_SEQUENCE=GGTCATTGGGCTCAACCTAG PRIMER_INTERNAL_4_SEQUENCE=AAGTCCCTGCGTGGTATCTG PRIMER_LEFT_4=31,19 PRIMER_RIGHT_4=118,20 PRIMER_INTERNAL_4=56,20 PRIMER_LEFT_4_TM=60.050 PRIMER_RIGHT_4_TM=59.550 PRIMER_INTERNAL_4_TM=60.134 PRIMER_LEFT_4_GC_PERCENT=57.895 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=7.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=88 = SEQUENCE_ID=MH385 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTGAACAAATGCAGGGGTGTGGGTTGGAGTGGCGTGTCTCTACTAAAGGCTCCTGGGAAGGAGGGGTACCGAGNTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGNTAACNCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA SEQUENCE_INCLUDED_REGION=35,63 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH396 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTATGAGATTCAGTGTNTTGTCATCTATCCCATCTCATCACCCACCCTTCTCTAACTTGGTTACTCTTTTCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT SEQUENCE_INCLUDED_REGION=35,76 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH399 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATATTACTCCCTATAATTATAACAGACTACCAGACTGGATTTTAGGCTTGTCTGTTCCACTAAAGAACTGGTGACTCTGGGACTAGAATTTGTAAGATCAGTGTAGACTAGGATGGGATTTACAATTCTGGTGCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGA SEQUENCE_INCLUDED_REGION=36,138 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.405024 PRIMER_LEFT_0_PENALTY=2.219979 PRIMER_RIGHT_0_PENALTY=1.185045 PRIMER_INTERNAL_0_PENALTY=9.644191 PRIMER_LEFT_0_SEQUENCE=AAAGAACTGGTGACTCTGGGA PRIMER_RIGHT_0_SEQUENCE=TGGCACCAGAATTGTAAATCC PRIMER_INTERNAL_0_SEQUENCE=TTTGTAAGATCAGTGTAGACTAGGATG PRIMER_LEFT_0=99,21 PRIMER_RIGHT_0=173,21 PRIMER_INTERNAL_0=126,27 PRIMER_LEFT_0_TM=58.780 PRIMER_RIGHT_0_TM=59.815 PRIMER_INTERNAL_0_TM=57.356 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_INTERNAL_0_GC_PERCENT=37.037 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=3.539926 PRIMER_LEFT_1_PENALTY=2.354881 PRIMER_RIGHT_1_PENALTY=1.185045 PRIMER_INTERNAL_1_PENALTY=9.644191 PRIMER_LEFT_1_SEQUENCE=AAAGAACTGGTGACTCTGGGAC PRIMER_RIGHT_1_SEQUENCE=TGGCACCAGAATTGTAAATCC PRIMER_INTERNAL_1_SEQUENCE=TTTGTAAGATCAGTGTAGACTAGGATG PRIMER_LEFT_1=99,22 PRIMER_RIGHT_1=173,21 PRIMER_INTERNAL_1=126,27 PRIMER_LEFT_1_TM=59.645 PRIMER_RIGHT_1_TM=59.815 PRIMER_INTERNAL_1_TM=57.356 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_INTERNAL_1_GC_PERCENT=37.037 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=9.1000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=4.048802 PRIMER_LEFT_2_PENALTY=2.863756 PRIMER_RIGHT_2_PENALTY=1.185045 PRIMER_INTERNAL_2_PENALTY=2.219979 PRIMER_LEFT_2_SEQUENCE=GGATTTTAGGCTTGTCTGTTCC PRIMER_RIGHT_2_SEQUENCE=GCACCAGAATTGTAAATCCCA PRIMER_INTERNAL_2_SEQUENCE=AAAGAACTGGTGACTCTGGGA PRIMER_LEFT_2=74,22 PRIMER_RIGHT_2=171,21 PRIMER_INTERNAL_2=99,21 PRIMER_LEFT_2_TM=59.136 PRIMER_RIGHT_2_TM=59.815 PRIMER_INTERNAL_2_TM=58.780 PRIMER_LEFT_2_GC_PERCENT=45.455 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=98 PRIMER_PAIR_3_PENALTY=4.048802 PRIMER_LEFT_3_PENALTY=2.863756 PRIMER_RIGHT_3_PENALTY=1.185045 PRIMER_INTERNAL_3_PENALTY=2.219979 PRIMER_LEFT_3_SEQUENCE=GGATTTTAGGCTTGTCTGTTCC PRIMER_RIGHT_3_SEQUENCE=TGGCACCAGAATTGTAAATCC PRIMER_INTERNAL_3_SEQUENCE=AAAGAACTGGTGACTCTGGGA PRIMER_LEFT_3=74,22 PRIMER_RIGHT_3=173,21 PRIMER_INTERNAL_3=99,21 PRIMER_LEFT_3_TM=59.136 PRIMER_RIGHT_3_TM=59.815 PRIMER_INTERNAL_3_TM=58.780 PRIMER_LEFT_3_GC_PERCENT=45.455 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_INTERNAL_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=4.347765 PRIMER_LEFT_4_PENALTY=3.162720 PRIMER_RIGHT_4_PENALTY=1.185045 PRIMER_INTERNAL_4_PENALTY=9.644191 PRIMER_LEFT_4_SEQUENCE=TAAAGAACTGGTGACTCTGGGA PRIMER_RIGHT_4_SEQUENCE=TGGCACCAGAATTGTAAATCC PRIMER_INTERNAL_4_SEQUENCE=TTTGTAAGATCAGTGTAGACTAGGATG PRIMER_LEFT_4=98,22 PRIMER_RIGHT_4=173,21 PRIMER_INTERNAL_4=126,27 PRIMER_LEFT_4_TM=58.837 PRIMER_RIGHT_4_TM=59.815 PRIMER_INTERNAL_4_TM=57.356 PRIMER_LEFT_4_GC_PERCENT=45.455 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=37.037 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH426 SEQUENCE_TEMPLATE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCGGACATGGTGGTGGGCACTGTAGTCCCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTNAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGC SEQUENCE_INCLUDED_REGION=35,29 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH429 SEQUENCE_TEMPLATE=CTCTATACATATTTAAAATTTTTNTTGTTACAAAGATAACTGTATCAAGGTAGAGGNTAAAACATATTTNATTTAACTTTTTGTTGTCATTGTTGTTGTTGTTTTGAGATGGAGTNTCGCTCTGTCGCCCAGGTTGGAGTGCAGTGGTGTGATCTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG SEQUENCE_INCLUDED_REGION=0,158 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.245683 PRIMER_LEFT_0_PENALTY=4.147950 PRIMER_RIGHT_0_PENALTY=2.097734 PRIMER_INTERNAL_0_PENALTY=4.867800 PRIMER_LEFT_0_SEQUENCE=TTTTTGTTGTCATTGTTGTTGTTG PRIMER_RIGHT_0_SEQUENCE=CTGAGATCACACCACTGCACT PRIMER_INTERNAL_0_SEQUENCE=CTCTGTCGCCCAGGTTGG PRIMER_LEFT_0=77,24 PRIMER_RIGHT_0=157,21 PRIMER_INTERNAL_0=119,18 PRIMER_LEFT_0_TM=59.852 PRIMER_RIGHT_0_TM=58.902 PRIMER_INTERNAL_0_TM=62.868 PRIMER_LEFT_0_GC_PERCENT=29.167 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_INTERNAL_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=6.428273 PRIMER_LEFT_1_PENALTY=4.330540 PRIMER_RIGHT_1_PENALTY=2.097734 PRIMER_INTERNAL_1_PENALTY=4.867800 PRIMER_LEFT_1_SEQUENCE=TTGTCATTGTTGTTGTTGTTTTGA PRIMER_RIGHT_1_SEQUENCE=CTGAGATCACACCACTGCACT PRIMER_INTERNAL_1_SEQUENCE=CTCTGTCGCCCAGGTTGG PRIMER_LEFT_1=83,24 PRIMER_RIGHT_1=157,21 PRIMER_INTERNAL_1=119,18 PRIMER_LEFT_1_TM=60.331 PRIMER_RIGHT_1_TM=58.902 PRIMER_INTERNAL_1_TM=62.868 PRIMER_LEFT_1_GC_PERCENT=29.167 PRIMER_RIGHT_1_GC_PERCENT=52.381 PRIMER_INTERNAL_1_GC_PERCENT=66.667 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=6.434184 PRIMER_LEFT_2_PENALTY=4.147950 PRIMER_RIGHT_2_PENALTY=2.286234 PRIMER_INTERNAL_2_PENALTY=4.867800 PRIMER_LEFT_2_SEQUENCE=TTTTTGTTGTCATTGTTGTTGTTG PRIMER_RIGHT_2_SEQUENCE=TGAGATCACACCACTGCACT PRIMER_INTERNAL_2_SEQUENCE=CTCTGTCGCCCAGGTTGG PRIMER_LEFT_2=77,24 PRIMER_RIGHT_2=156,20 PRIMER_INTERNAL_2=119,18 PRIMER_LEFT_2_TM=59.852 PRIMER_RIGHT_2_TM=57.714 PRIMER_INTERNAL_2_TM=62.868 PRIMER_LEFT_2_GC_PERCENT=29.167 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=66.667 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=6.434184 PRIMER_LEFT_3_PENALTY=4.147950 PRIMER_RIGHT_3_PENALTY=2.286234 PRIMER_INTERNAL_3_PENALTY=4.867800 PRIMER_LEFT_3_SEQUENCE=TTTTTGTTGTCATTGTTGTTGTTG PRIMER_RIGHT_3_SEQUENCE=CTGAGATCACACCACTGCAC PRIMER_INTERNAL_3_SEQUENCE=CTCTGTCGCCCAGGTTGG PRIMER_LEFT_3=77,24 PRIMER_RIGHT_3=157,20 PRIMER_INTERNAL_3=119,18 PRIMER_LEFT_3_TM=59.852 PRIMER_RIGHT_3_TM=57.714 PRIMER_INTERNAL_3_TM=62.868 PRIMER_LEFT_3_GC_PERCENT=29.167 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=66.667 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=81 PRIMER_PAIR_4_PENALTY=6.546818 PRIMER_LEFT_4_PENALTY=4.449085 PRIMER_RIGHT_4_PENALTY=2.097734 PRIMER_INTERNAL_4_PENALTY=4.867800 PRIMER_LEFT_4_SEQUENCE=TTGTCATTGTTGTTGTTGTTTTG PRIMER_RIGHT_4_SEQUENCE=CTGAGATCACACCACTGCACT PRIMER_INTERNAL_4_SEQUENCE=CTCTGTCGCCCAGGTTGG PRIMER_LEFT_4=83,23 PRIMER_RIGHT_4=157,21 PRIMER_INTERNAL_4=119,18 PRIMER_LEFT_4_TM=58.551 PRIMER_RIGHT_4_TM=58.902 PRIMER_INTERNAL_4_TM=62.868 PRIMER_LEFT_4_GC_PERCENT=30.435 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=66.667 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.6000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH436 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCAGAAAACAAAAATTTCGTACAGTNCAAATAACATTTGGGAATTCCTTAAGTNACCCATAAAGTACAATATTCGATGTTTCGTGTNNACAACCCCAGGAAAAATGTTTAATGCCCGTAGTAATGTACCTGATAGGAAAAAAAGTGCATATGCCAAAATAGAATGTTTGATGTAAAGGAGAGACAGAGAGAGGAAAGAGAGAGAGAAAGAGAGAGAGACGAAAGAGAGAGAGGAAG SEQUENCE_INCLUDED_REGION=34,236 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.982775 PRIMER_LEFT_0_PENALTY=0.699731 PRIMER_RIGHT_0_PENALTY=4.283044 PRIMER_INTERNAL_0_PENALTY=3.932182 PRIMER_LEFT_0_SEQUENCE=GGAAAAATGTTTAATGCCCG PRIMER_RIGHT_0_SEQUENCE=CATCAAACATTCTATTTTGGCA PRIMER_INTERNAL_0_SEQUENCE=CCTGATAGGAAAAAAAGTGCA PRIMER_LEFT_0=131,20 PRIMER_RIGHT_0=205,22 PRIMER_INTERNAL_0=161,21 PRIMER_LEFT_0_TM=59.300 PRIMER_RIGHT_0_TM=57.717 PRIMER_INTERNAL_0_TM=57.068 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=31.818 PRIMER_INTERNAL_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=12.9000 PRIMER_RIGHT_0_END_STABILITY=10.0000 PRIMER_PAIR_0_COMPL_ANY=9.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=5.133665 PRIMER_LEFT_1_PENALTY=1.043085 PRIMER_RIGHT_1_PENALTY=4.090580 PRIMER_INTERNAL_1_PENALTY=4.222735 PRIMER_LEFT_1_SEQUENCE=GGAAAAAAAGTGCATATGCCA PRIMER_RIGHT_1_SEQUENCE=TCCTCTCTCTCTTTCGTCTCTCTC PRIMER_INTERNAL_1_SEQUENCE=GGAGAGACAGAGAGAGGAAAGAGA PRIMER_LEFT_1=168,21 PRIMER_RIGHT_1=267,24 PRIMER_INTERNAL_1=210,24 PRIMER_LEFT_1_TM=59.957 PRIMER_RIGHT_1_TM=59.909 PRIMER_INTERNAL_1_TM=59.777 PRIMER_LEFT_1_GC_PERCENT=38.095 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=10.0000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=5.144079 PRIMER_LEFT_2_PENALTY=0.699731 PRIMER_RIGHT_2_PENALTY=4.444347 PRIMER_INTERNAL_2_PENALTY=3.932182 PRIMER_LEFT_2_SEQUENCE=GGAAAAATGTTTAATGCCCG PRIMER_RIGHT_2_SEQUENCE=ACATCAAACATTCTATTTTGGCA PRIMER_INTERNAL_2_SEQUENCE=CCTGATAGGAAAAAAAGTGCA PRIMER_LEFT_2=131,20 PRIMER_RIGHT_2=206,23 PRIMER_INTERNAL_2=161,21 PRIMER_LEFT_2_TM=59.300 PRIMER_RIGHT_2_TM=58.556 PRIMER_INTERNAL_2_TM=57.068 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=30.435 PRIMER_INTERNAL_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=12.9000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=9.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=76 PRIMER_PAIR_3_PENALTY=5.327400 PRIMER_LEFT_3_PENALTY=4.627669 PRIMER_RIGHT_3_PENALTY=0.699731 PRIMER_INTERNAL_3_PENALTY=6.371570 PRIMER_LEFT_3_SEQUENCE=CAAATAACATTTGGGAATTCCTT PRIMER_RIGHT_3_SEQUENCE=CGGGCATTAAACATTTTTCC PRIMER_INTERNAL_3_SEQUENCE=AAAGTACAATATTCGATGTTTCGTG PRIMER_LEFT_3=60,23 PRIMER_RIGHT_3=150,20 PRIMER_INTERNAL_3=94,25 PRIMER_LEFT_3_TM=58.372 PRIMER_RIGHT_3_TM=59.300 PRIMER_INTERNAL_3_TM=58.628 PRIMER_LEFT_3_GC_PERCENT=30.435 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=32.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=91 PRIMER_PAIR_4_PENALTY=5.351938 PRIMER_LEFT_4_PENALTY=1.068894 PRIMER_RIGHT_4_PENALTY=4.283044 PRIMER_INTERNAL_4_PENALTY=3.932182 PRIMER_LEFT_4_SEQUENCE=GGAAAAATGTTTAATGCCCGT PRIMER_RIGHT_4_SEQUENCE=CATCAAACATTCTATTTTGGCA PRIMER_INTERNAL_4_SEQUENCE=CCTGATAGGAAAAAAAGTGCA PRIMER_LEFT_4=131,21 PRIMER_RIGHT_4=205,22 PRIMER_INTERNAL_4=161,21 PRIMER_LEFT_4_TM=60.069 PRIMER_RIGHT_4_TM=57.717 PRIMER_INTERNAL_4_TM=57.068 PRIMER_LEFT_4_GC_PERCENT=38.095 PRIMER_RIGHT_4_GC_PERCENT=31.818 PRIMER_INTERNAL_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=11.1000 PRIMER_RIGHT_4_END_STABILITY=10.0000 PRIMER_PAIR_4_COMPL_ANY=9.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH437 SEQUENCE_TEMPLATE=TGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAGGACGGGCAAAGTTGAGTGGCTGAAAGTGTAGGGAACAGCATTTCATGCAGAGGGAATTGCATGTCCAAAGCCTGGCATGCAGCAAGC SEQUENCE_INCLUDED_REGION=37,93 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.127660 PRIMER_LEFT_0_PENALTY=1.046699 PRIMER_RIGHT_0_PENALTY=0.080962 PRIMER_INTERNAL_0_PENALTY=0.194598 PRIMER_LEFT_0_SEQUENCE=AAGGACGGGCAAAGTTGAGT PRIMER_RIGHT_0_SEQUENCE=AGGCTTTGGACATGCAATTC PRIMER_INTERNAL_0_SEQUENCE=GGAACAGCATTTCATGCAGA PRIMER_LEFT_0=40,20 PRIMER_RIGHT_0=115,20 PRIMER_INTERNAL_0=74,20 PRIMER_LEFT_0_TM=61.047 PRIMER_RIGHT_0_TM=60.081 PRIMER_INTERNAL_0_TM=59.805 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=1.306828 PRIMER_LEFT_1_PENALTY=1.239392 PRIMER_RIGHT_1_PENALTY=0.067436 PRIMER_INTERNAL_1_PENALTY=1.276629 PRIMER_LEFT_1_SEQUENCE=CTGAAGGACGGGCAAAGTT PRIMER_RIGHT_1_SEQUENCE=CTTTGGACATGCAATTCCCT PRIMER_INTERNAL_1_SEQUENCE=TAGGGAACAGCATTTCATGC PRIMER_LEFT_1=37,19 PRIMER_RIGHT_1=112,20 PRIMER_INTERNAL_1=71,20 PRIMER_LEFT_1_TM=60.239 PRIMER_RIGHT_1_TM=59.933 PRIMER_INTERNAL_1_TM=58.723 PRIMER_LEFT_1_GC_PERCENT=52.632 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=1.320353 PRIMER_LEFT_2_PENALTY=1.239392 PRIMER_RIGHT_2_PENALTY=0.080962 PRIMER_INTERNAL_2_PENALTY=0.194598 PRIMER_LEFT_2_SEQUENCE=AAGGACGGGCAAAGTTGAG PRIMER_RIGHT_2_SEQUENCE=AGGCTTTGGACATGCAATTC PRIMER_INTERNAL_2_SEQUENCE=GGAACAGCATTTCATGCAGA PRIMER_LEFT_2=40,19 PRIMER_RIGHT_2=115,20 PRIMER_INTERNAL_2=74,20 PRIMER_LEFT_2_TM=60.239 PRIMER_RIGHT_2_TM=60.081 PRIMER_INTERNAL_2_TM=59.805 PRIMER_LEFT_2_GC_PERCENT=52.632 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=76 PRIMER_PAIR_3_PENALTY=1.320353 PRIMER_LEFT_3_PENALTY=1.239392 PRIMER_RIGHT_3_PENALTY=0.080962 PRIMER_INTERNAL_3_PENALTY=0.194598 PRIMER_LEFT_3_SEQUENCE=CTGAAGGACGGGCAAAGTT PRIMER_RIGHT_3_SEQUENCE=AGGCTTTGGACATGCAATTC PRIMER_INTERNAL_3_SEQUENCE=GGAACAGCATTTCATGCAGA PRIMER_LEFT_3=37,19 PRIMER_RIGHT_3=115,20 PRIMER_INTERNAL_3=74,20 PRIMER_LEFT_3_TM=60.239 PRIMER_RIGHT_3_TM=60.081 PRIMER_INTERNAL_3_TM=59.805 PRIMER_LEFT_3_GC_PERCENT=52.632 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=1.368717 PRIMER_LEFT_4_PENALTY=1.287755 PRIMER_RIGHT_4_PENALTY=0.080962 PRIMER_INTERNAL_4_PENALTY=0.194598 PRIMER_LEFT_4_SEQUENCE=AGGACGGGCAAAGTTGAGT PRIMER_RIGHT_4_SEQUENCE=AGGCTTTGGACATGCAATTC PRIMER_INTERNAL_4_SEQUENCE=GGAACAGCATTTCATGCAGA PRIMER_LEFT_4=41,19 PRIMER_RIGHT_4=115,20 PRIMER_INTERNAL_4=74,20 PRIMER_LEFT_4_TM=59.712 PRIMER_RIGHT_4_TM=60.081 PRIMER_INTERNAL_4_TM=59.805 PRIMER_LEFT_4_GC_PERCENT=52.632 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH441 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATACTAGGAACTCAGTTCCTTTTTCTTAAAACAACATAAGACAGGAAAACATCACATCCTTTAGGTNNCGAAGACTGAGCCCATTTTAAACGCCGTTATTTTNCCCANCCCACCGNCCCAAGCCCCCCGCCCCCCGCCCCGAGCGAATGCTGTTATAACTGGTTTATCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAG SEQUENCE_INCLUDED_REGION=36,134 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=7.224529 PRIMER_LEFT_0_PENALTY=6.453153 PRIMER_RIGHT_0_PENALTY=0.771376 PRIMER_INTERNAL_0_PENALTY=3.615875 PRIMER_LEFT_0_SEQUENCE=GGAACTCAGTTCCTTTTTCTTAAAA PRIMER_RIGHT_0_SEQUENCE=GCGTTTAAAATGGGCTCAGT PRIMER_INTERNAL_0_SEQUENCE=GACAGGAAAACATCACATCCTTT PRIMER_LEFT_0=44,25 PRIMER_RIGHT_0=130,20 PRIMER_INTERNAL_0=77,23 PRIMER_LEFT_0_TM=58.547 PRIMER_RIGHT_0_TM=59.229 PRIMER_INTERNAL_0_TM=59.384 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=39.130 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=87 PRIMER_PAIR_1_PENALTY=7.246225 PRIMER_LEFT_1_PENALTY=6.474849 PRIMER_RIGHT_1_PENALTY=0.771376 PRIMER_INTERNAL_1_PENALTY=3.615875 PRIMER_LEFT_1_SEQUENCE=TCAGTTCCTTTTTCTTAAAACAACA PRIMER_RIGHT_1_SEQUENCE=GCGTTTAAAATGGGCTCAGT PRIMER_INTERNAL_1_SEQUENCE=GACAGGAAAACATCACATCCTTT PRIMER_LEFT_1=49,25 PRIMER_RIGHT_1=130,20 PRIMER_INTERNAL_1=77,23 PRIMER_LEFT_1_TM=58.525 PRIMER_RIGHT_1_TM=59.229 PRIMER_INTERNAL_1_TM=59.384 PRIMER_LEFT_1_GC_PERCENT=28.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=7.423825 PRIMER_LEFT_2_PENALTY=6.652448 PRIMER_RIGHT_2_PENALTY=0.771376 PRIMER_INTERNAL_2_PENALTY=3.615875 PRIMER_LEFT_2_SEQUENCE=GGAACTCAGTTCCTTTTTCTTAAA PRIMER_RIGHT_2_SEQUENCE=GCGTTTAAAATGGGCTCAGT PRIMER_INTERNAL_2_SEQUENCE=GACAGGAAAACATCACATCCTTT PRIMER_LEFT_2=44,24 PRIMER_RIGHT_2=130,20 PRIMER_INTERNAL_2=77,23 PRIMER_LEFT_2_TM=57.348 PRIMER_RIGHT_2_TM=59.229 PRIMER_INTERNAL_2_TM=59.384 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=11.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=87 PRIMER_PAIR_3_PENALTY=7.535615 PRIMER_LEFT_3_PENALTY=6.453153 PRIMER_RIGHT_3_PENALTY=1.082462 PRIMER_INTERNAL_3_PENALTY=3.615875 PRIMER_LEFT_3_SEQUENCE=GGAACTCAGTTCCTTTTTCTTAAAA PRIMER_RIGHT_3_SEQUENCE=AATAACGGCGTTTAAAATGGG PRIMER_INTERNAL_3_SEQUENCE=GACAGGAAAACATCACATCCTTT PRIMER_LEFT_3=44,25 PRIMER_RIGHT_3=137,21 PRIMER_INTERNAL_3=77,23 PRIMER_LEFT_3_TM=58.547 PRIMER_RIGHT_3_TM=60.082 PRIMER_INTERNAL_3_TM=59.384 PRIMER_LEFT_3_GC_PERCENT=32.000 PRIMER_RIGHT_3_GC_PERCENT=38.095 PRIMER_INTERNAL_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=11.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=9.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=7.557311 PRIMER_LEFT_4_PENALTY=6.474849 PRIMER_RIGHT_4_PENALTY=1.082462 PRIMER_INTERNAL_4_PENALTY=3.615875 PRIMER_LEFT_4_SEQUENCE=TCAGTTCCTTTTTCTTAAAACAACA PRIMER_RIGHT_4_SEQUENCE=AATAACGGCGTTTAAAATGGG PRIMER_INTERNAL_4_SEQUENCE=GACAGGAAAACATCACATCCTTT PRIMER_LEFT_4=49,25 PRIMER_RIGHT_4=137,21 PRIMER_INTERNAL_4=77,23 PRIMER_LEFT_4_TM=58.525 PRIMER_RIGHT_4_TM=60.082 PRIMER_INTERNAL_4_TM=59.384 PRIMER_LEFT_4_GC_PERCENT=28.000 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_INTERNAL_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=9.6000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH459 SEQUENCE_TEMPLATE=AGACAACCCAGACAGTGAGACACTAATCAGGAGTCTAATACAATACAAAAGAGCAATGAGCATATGAACCATCATATGCTGTTATCATTGAGCCACTTATACCCTGCATGATCAAGCACCCAGTGCCCTTGTAGTCTACATCCCAGTACGAATAAAAGCCAGAGTATTGACTCTCTTTAAAAGGTAAATTTTAATTTTTTCCTTACCACTAAGTATAATTCAGATAGCCCAGG SEQUENCE_INCLUDED_REGION=0,233 P3_COMMENT=CL is CAGACAGTGAGACACTAATCAGG P3_COMMENT=CR is AGTCAATACTCTGGCTTTTATTCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.829603 PRIMER_LEFT_0_PENALTY=2.262496 PRIMER_RIGHT_0_PENALTY=0.567107 PRIMER_INTERNAL_0_PENALTY=0.703016 PRIMER_LEFT_0_SEQUENCE=GAGCAATGAGCATATGAACCA PRIMER_RIGHT_0_SEQUENCE=GACTACAAGGGCACTGGGTG PRIMER_INTERNAL_0_SEQUENCE=TGAGCCACTTATACCCTGCA PRIMER_LEFT_0=50,21 PRIMER_RIGHT_0=135,20 PRIMER_INTERNAL_0=88,20 PRIMER_LEFT_0_TM=58.738 PRIMER_RIGHT_0_TM=60.567 PRIMER_INTERNAL_0_TM=59.297 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=86 PRIMER_PAIR_1_PENALTY=2.831250 PRIMER_LEFT_1_PENALTY=2.264143 PRIMER_RIGHT_1_PENALTY=0.567107 PRIMER_INTERNAL_1_PENALTY=0.703016 PRIMER_LEFT_1_SEQUENCE=AGAGCAATGAGCATATGAACCA PRIMER_RIGHT_1_SEQUENCE=GACTACAAGGGCACTGGGTG PRIMER_INTERNAL_1_SEQUENCE=TGAGCCACTTATACCCTGCA PRIMER_LEFT_1=49,22 PRIMER_RIGHT_1=135,20 PRIMER_INTERNAL_1=88,20 PRIMER_LEFT_1_TM=59.736 PRIMER_RIGHT_1_TM=60.567 PRIMER_INTERNAL_1_TM=59.297 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=87 PRIMER_PAIR_2_PENALTY=3.184342 PRIMER_LEFT_2_PENALTY=0.703016 PRIMER_RIGHT_2_PENALTY=2.481326 PRIMER_INTERNAL_2_PENALTY=0.567107 PRIMER_LEFT_2_SEQUENCE=TGAGCCACTTATACCCTGCA PRIMER_RIGHT_2_SEQUENCE=TCTGGCTTTTATTCGTACTGGG PRIMER_INTERNAL_2_SEQUENCE=CACCCAGTGCCCTTGTAGTC PRIMER_LEFT_2=88,20 PRIMER_RIGHT_2=162,22 PRIMER_INTERNAL_2=116,20 PRIMER_LEFT_2_TM=59.297 PRIMER_RIGHT_2_TM=60.481 PRIMER_INTERNAL_2_TM=60.567 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_INTERNAL_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=3.433889 PRIMER_LEFT_3_PENALTY=2.866782 PRIMER_RIGHT_3_PENALTY=0.567107 PRIMER_INTERNAL_3_PENALTY=0.703016 PRIMER_LEFT_3_SEQUENCE=GCAATGAGCATATGAACCATCA PRIMER_RIGHT_3_SEQUENCE=GACTACAAGGGCACTGGGTG PRIMER_INTERNAL_3_SEQUENCE=TGAGCCACTTATACCCTGCA PRIMER_LEFT_3=52,22 PRIMER_RIGHT_3=135,20 PRIMER_INTERNAL_3=88,20 PRIMER_LEFT_3_TM=60.867 PRIMER_RIGHT_3_TM=60.567 PRIMER_INTERNAL_3_TM=59.297 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=84 PRIMER_PAIR_4_PENALTY=3.493466 PRIMER_LEFT_4_PENALTY=2.926360 PRIMER_RIGHT_4_PENALTY=0.567107 PRIMER_INTERNAL_4_PENALTY=0.703016 PRIMER_LEFT_4_SEQUENCE=AGCAATGAGCATATGAACCATC PRIMER_RIGHT_4_SEQUENCE=GACTACAAGGGCACTGGGTG PRIMER_INTERNAL_4_SEQUENCE=TGAGCCACTTATACCCTGCA PRIMER_LEFT_4=51,22 PRIMER_RIGHT_4=135,20 PRIMER_INTERNAL_4=88,20 PRIMER_LEFT_4_TM=59.074 PRIMER_RIGHT_4_TM=60.567 PRIMER_INTERNAL_4_TM=59.297 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=85 = SEQUENCE_ID=MH465 SEQUENCE_TEMPLATE=CGNNNTTGATGCTGCAGGTCGACTCTAGAGGATCCCCCTATGTGCAAATCAGACCACAAGAGAGGANGGAAATTGAATCAATGGAATGAGAATTATTTGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCC SEQUENCE_INCLUDED_REGION=37,64 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH471 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTGCTCTGATTTTACCTCACCTCTAGAGTNNGTTTTATATGTAGACTACTTAAGGCACTTCCTTCGGACAGTGTTGGGAGAGACAGTGATAGATTTTTTTTCAGATGCCTGACTTGCTTTTTTTCAGATGTCATTACANTGTACTGAAATACTTTTTTCAAATACGTAATAATTCAGATAGATAACCATGTGTGTTCAGGGGACCCCCAGATGATCCTGAGGTTCAGTGACTCACCAGAAGTACCCACGGAACTCAGCATATAGAAACATGTTTTAGTTTGCTGAACTTTTATCCTGTTATTTTACTGAAG SEQUENCE_INCLUDED_REGION=36,313 P3_COMMENT=CL is GTGCTCTGATTTTACCTCACC P3_COMMENT=CR is TCTATATGCTGAGTTCCGTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.704234 PRIMER_LEFT_0_PENALTY=0.700912 PRIMER_RIGHT_0_PENALTY=0.003322 PRIMER_INTERNAL_0_PENALTY=0.539612 PRIMER_LEFT_0_SEQUENCE=ACCATGTGTGTTCAGGGGAC PRIMER_RIGHT_0_SEQUENCE=ATGCTGAGTTCCGTGGGTAC PRIMER_INTERNAL_0_SEQUENCE=CCCAGATGATCCTGAGGTTC PRIMER_LEFT_0=222,20 PRIMER_RIGHT_0=297,20 PRIMER_INTERNAL_0=243,20 PRIMER_LEFT_0_TM=60.701 PRIMER_RIGHT_0_TM=59.997 PRIMER_INTERNAL_0_TM=59.460 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=7.00 PRIMER_LEFT_0_END_STABILITY=9.1000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=0.740939 PRIMER_LEFT_1_PENALTY=0.737617 PRIMER_RIGHT_1_PENALTY=0.003322 PRIMER_INTERNAL_1_PENALTY=0.539612 PRIMER_LEFT_1_SEQUENCE=TAACCATGTGTGTTCAGGGG PRIMER_RIGHT_1_SEQUENCE=ATGCTGAGTTCCGTGGGTAC PRIMER_INTERNAL_1_SEQUENCE=CCCAGATGATCCTGAGGTTC PRIMER_LEFT_1=220,20 PRIMER_RIGHT_1=297,20 PRIMER_INTERNAL_1=243,20 PRIMER_LEFT_1_TM=59.262 PRIMER_RIGHT_1_TM=59.997 PRIMER_INTERNAL_1_TM=59.460 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=7.00 PRIMER_LEFT_1_END_STABILITY=10.9000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=1.218783 PRIMER_LEFT_2_PENALTY=1.215461 PRIMER_RIGHT_2_PENALTY=0.003322 PRIMER_INTERNAL_2_PENALTY=0.539612 PRIMER_LEFT_2_SEQUENCE=CCATGTGTGTTCAGGGGAC PRIMER_RIGHT_2_SEQUENCE=ATGCTGAGTTCCGTGGGTAC PRIMER_INTERNAL_2_SEQUENCE=CCCAGATGATCCTGAGGTTC PRIMER_LEFT_2=223,19 PRIMER_RIGHT_2=297,20 PRIMER_INTERNAL_2=243,20 PRIMER_LEFT_2_TM=59.785 PRIMER_RIGHT_2_TM=59.997 PRIMER_INTERNAL_2_TM=59.460 PRIMER_LEFT_2_GC_PERCENT=57.895 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=7.00 PRIMER_LEFT_2_END_STABILITY=9.1000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=1.218783 PRIMER_LEFT_3_PENALTY=1.215461 PRIMER_RIGHT_3_PENALTY=0.003322 PRIMER_INTERNAL_3_PENALTY=0.539612 PRIMER_LEFT_3_SEQUENCE=ACCATGTGTGTTCAGGGGA PRIMER_RIGHT_3_SEQUENCE=ATGCTGAGTTCCGTGGGTAC PRIMER_INTERNAL_3_SEQUENCE=CCCAGATGATCCTGAGGTTC PRIMER_LEFT_3=222,19 PRIMER_RIGHT_3=297,20 PRIMER_INTERNAL_3=243,20 PRIMER_LEFT_3_TM=59.785 PRIMER_RIGHT_3_TM=59.997 PRIMER_INTERNAL_3_TM=59.460 PRIMER_LEFT_3_GC_PERCENT=52.632 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=7.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=1.241404 PRIMER_LEFT_4_PENALTY=1.238082 PRIMER_RIGHT_4_PENALTY=0.003322 PRIMER_INTERNAL_4_PENALTY=0.539612 PRIMER_LEFT_4_SEQUENCE=AACCATGTGTGTTCAGGGGA PRIMER_RIGHT_4_SEQUENCE=ATGCTGAGTTCCGTGGGTAC PRIMER_INTERNAL_4_SEQUENCE=CCCAGATGATCCTGAGGTTC PRIMER_LEFT_4=221,20 PRIMER_RIGHT_4=297,20 PRIMER_INTERNAL_4=243,20 PRIMER_LEFT_4_TM=61.238 PRIMER_RIGHT_4_TM=59.997 PRIMER_INTERNAL_4_TM=59.460 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=7.00 PRIMER_LEFT_4_END_STABILITY=10.9000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=77 = SEQUENCE_ID=MH476 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGGCCCTTCCCGGCATCAACCACCAGGCCCGAGTGTGGGGCCCGAGTTGCTGGCTGCCCTGAGTGCCCTGGTCCCTGGGGCCCCCATGGGAGATCAAGGGGCTCAGGAGAGGGCATGGGACTCAGGAGGTCCCGGGGCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTNAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG SEQUENCE_INCLUDED_REGION=36,141 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.554176 PRIMER_LEFT_0_PENALTY=3.262057 PRIMER_RIGHT_0_PENALTY=2.292120 PRIMER_INTERNAL_0_PENALTY=1.271648 PRIMER_LEFT_0_SEQUENCE=CTGAGTGCCCTGGTCCCT PRIMER_RIGHT_0_SEQUENCE=GGGACCTCCTGAGTCCCAT PRIMER_INTERNAL_0_SEQUENCE=GATCAAGGGGCTCAGGAGAG PRIMER_LEFT_0=96,18 PRIMER_RIGHT_0=170,19 PRIMER_INTERNAL_0=129,20 PRIMER_LEFT_0_TM=61.262 PRIMER_RIGHT_0_TM=61.292 PRIMER_INTERNAL_0_TM=61.272 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=9.6000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=6.317680 PRIMER_LEFT_1_PENALTY=3.262057 PRIMER_RIGHT_1_PENALTY=3.055624 PRIMER_INTERNAL_1_PENALTY=1.271648 PRIMER_LEFT_1_SEQUENCE=CTGAGTGCCCTGGTCCCT PRIMER_RIGHT_1_SEQUENCE=GGGACCTCCTGAGTCCCA PRIMER_INTERNAL_1_SEQUENCE=GATCAAGGGGCTCAGGAGAG PRIMER_LEFT_1=96,18 PRIMER_RIGHT_1=170,18 PRIMER_INTERNAL_1=129,20 PRIMER_LEFT_1_TM=61.262 PRIMER_RIGHT_1_TM=61.056 PRIMER_INTERNAL_1_TM=61.272 PRIMER_LEFT_1_GC_PERCENT=66.667 PRIMER_RIGHT_1_GC_PERCENT=66.667 PRIMER_INTERNAL_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=6.847421 PRIMER_LEFT_2_PENALTY=4.486184 PRIMER_RIGHT_2_PENALTY=2.361236 PRIMER_INTERNAL_2_PENALTY=3.262057 PRIMER_LEFT_2_SEQUENCE=CTTCCCGGCATCAACCAC PRIMER_RIGHT_2_SEQUENCE=GAGCCCCTTGATCTCCCAT PRIMER_INTERNAL_2_SEQUENCE=CTGAGTGCCCTGGTCCCT PRIMER_LEFT_2=43,18 PRIMER_RIGHT_2=141,19 PRIMER_INTERNAL_2=96,18 PRIMER_LEFT_2_TM=62.486 PRIMER_RIGHT_2_TM=61.361 PRIMER_INTERNAL_2_TM=61.262 PRIMER_LEFT_2_GC_PERCENT=61.111 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_INTERNAL_2_GC_PERCENT=66.667 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=7.6000 PRIMER_RIGHT_2_END_STABILITY=9.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=7.071457 PRIMER_LEFT_3_PENALTY=4.486184 PRIMER_RIGHT_3_PENALTY=2.585272 PRIMER_INTERNAL_3_PENALTY=3.262057 PRIMER_LEFT_3_SEQUENCE=CTTCCCGGCATCAACCAC PRIMER_RIGHT_3_SEQUENCE=AGCCCCTTGATCTCCCAT PRIMER_INTERNAL_3_SEQUENCE=CTGAGTGCCCTGGTCCCT PRIMER_LEFT_3=43,18 PRIMER_RIGHT_3=140,18 PRIMER_INTERNAL_3=96,18 PRIMER_LEFT_3_TM=62.486 PRIMER_RIGHT_3_TM=59.415 PRIMER_INTERNAL_3_TM=61.262 PRIMER_LEFT_3_GC_PERCENT=61.111 PRIMER_RIGHT_3_GC_PERCENT=55.556 PRIMER_INTERNAL_3_GC_PERCENT=66.667 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=9.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=7.623076 PRIMER_LEFT_4_PENALTY=4.486184 PRIMER_RIGHT_4_PENALTY=3.136891 PRIMER_INTERNAL_4_PENALTY=3.262057 PRIMER_LEFT_4_SEQUENCE=CTTCCCGGCATCAACCAC PRIMER_RIGHT_4_SEQUENCE=GAGCCCCTTGATCTCCCA PRIMER_INTERNAL_4_SEQUENCE=CTGAGTGCCCTGGTCCCT PRIMER_LEFT_4=43,18 PRIMER_RIGHT_4=141,18 PRIMER_INTERNAL_4=96,18 PRIMER_LEFT_4_TM=62.486 PRIMER_RIGHT_4_TM=61.137 PRIMER_INTERNAL_4_TM=61.262 PRIMER_LEFT_4_GC_PERCENT=61.111 PRIMER_RIGHT_4_GC_PERCENT=61.111 PRIMER_INTERNAL_4_GC_PERCENT=66.667 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=7.6000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = SEQUENCE_ID=MH479 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGATTTCAGAATCACTATAGACAAGTGACCGCTATTGTTTCCTCTTCTCCCCTTTATAAATGGGAGAATTTACTATCCTACGCCTATTTCACCATTGTATATTGGTTCTACATGAGTAGATCACGTGTCTCTTTAGTTCACAAGTCTTTAAGAGGAAATGCACTTGAATATGGGCTGGACTTTGTGATTTGCTCCATCATGAAAATGTAGAGGAAGTAATATTCTAGGACTTCCAAGGCTAACTTATGAAAAGCCTTGCAGTTTTTGTCTGGATGTCTGCATCTCTTGGCATGTTTCTTCCTTAGCAACTCCC SEQUENCE_INCLUDED_REGION=36,315 P3_COMMENT=CL is TTGTTTCCTCTTCTCCCCTT P3_COMMENT=CR is GCTAAGGAAGAAACATGCCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.452342 PRIMER_LEFT_0_PENALTY=0.645726 PRIMER_RIGHT_0_PENALTY=0.806616 PRIMER_INTERNAL_0_PENALTY=4.026278 PRIMER_LEFT_0_SEQUENCE=GGCTGGACTTTGTGATTTGC PRIMER_RIGHT_0_SEQUENCE=CCAGACAAAAACTGCAAGGC PRIMER_INTERNAL_0_SEQUENCE=GGACTTCCAAGGCTAACTTATGAA PRIMER_LEFT_0=210,20 PRIMER_RIGHT_0=309,20 PRIMER_INTERNAL_0=264,24 PRIMER_LEFT_0_TM=60.646 PRIMER_RIGHT_0_TM=60.807 PRIMER_INTERNAL_0_TM=60.026 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=1.871422 PRIMER_LEFT_1_PENALTY=1.064806 PRIMER_RIGHT_1_PENALTY=0.806616 PRIMER_INTERNAL_1_PENALTY=4.026278 PRIMER_LEFT_1_SEQUENCE=TGTGATTTGCTCCATCATGAA PRIMER_RIGHT_1_SEQUENCE=CCAGACAAAAACTGCAAGGC PRIMER_INTERNAL_1_SEQUENCE=GGACTTCCAAGGCTAACTTATGAA PRIMER_LEFT_1=220,21 PRIMER_RIGHT_1=309,20 PRIMER_INTERNAL_1=264,24 PRIMER_LEFT_1_TM=60.065 PRIMER_RIGHT_1_TM=60.807 PRIMER_INTERNAL_1_TM=60.026 PRIMER_LEFT_1_GC_PERCENT=38.095 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=90 PRIMER_PAIR_2_PENALTY=1.871422 PRIMER_LEFT_2_PENALTY=1.064806 PRIMER_RIGHT_2_PENALTY=0.806616 PRIMER_INTERNAL_2_PENALTY=4.026278 PRIMER_LEFT_2_SEQUENCE=TTGTGATTTGCTCCATCATGA PRIMER_RIGHT_2_SEQUENCE=CCAGACAAAAACTGCAAGGC PRIMER_INTERNAL_2_SEQUENCE=GGACTTCCAAGGCTAACTTATGAA PRIMER_LEFT_2=219,21 PRIMER_RIGHT_2=309,20 PRIMER_INTERNAL_2=264,24 PRIMER_LEFT_2_TM=60.065 PRIMER_RIGHT_2_TM=60.807 PRIMER_INTERNAL_2_TM=60.026 PRIMER_LEFT_2_GC_PERCENT=38.095 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=91 PRIMER_PAIR_3_PENALTY=1.889809 PRIMER_LEFT_3_PENALTY=0.495173 PRIMER_RIGHT_3_PENALTY=1.394636 PRIMER_INTERNAL_3_PENALTY=4.431064 PRIMER_LEFT_3_SEQUENCE=GGGCTGGACTTTGTGATTTG PRIMER_RIGHT_3_SEQUENCE=CAAAAACTGCAAGGCTTTTCA PRIMER_INTERNAL_3_SEQUENCE=TCCATCATGAAAATGTAGAGGAA PRIMER_LEFT_3=209,20 PRIMER_RIGHT_3=304,21 PRIMER_INTERNAL_3=230,23 PRIMER_LEFT_3_TM=60.495 PRIMER_RIGHT_3_TM=60.395 PRIMER_INTERNAL_3_TM=58.569 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=38.095 PRIMER_INTERNAL_3_GC_PERCENT=34.783 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=96 PRIMER_PAIR_4_PENALTY=1.889809 PRIMER_LEFT_4_PENALTY=0.495173 PRIMER_RIGHT_4_PENALTY=1.394636 PRIMER_INTERNAL_4_PENALTY=3.020535 PRIMER_LEFT_4_SEQUENCE=TGGGCTGGACTTTGTGATTT PRIMER_RIGHT_4_SEQUENCE=CAAAAACTGCAAGGCTTTTCA PRIMER_INTERNAL_4_SEQUENCE=GCTCCATCATGAAAATGTAGAGG PRIMER_LEFT_4=208,20 PRIMER_RIGHT_4=304,21 PRIMER_INTERNAL_4=228,23 PRIMER_LEFT_4_TM=60.495 PRIMER_RIGHT_4_TM=60.395 PRIMER_INTERNAL_4_TM=59.979 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_INTERNAL_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=7.3000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=97 = SEQUENCE_ID=MH480 SEQUENCE_TEMPLATE=CAGTAACTTTTCATAAAGCAACCTCACCTCTACCATTATGGTGCTCACTGTCTGAATTATCCTCCTCTTCTAGAAACTATATTTACAGTTTGTCGATGATCCTGGAAATGACTACTCTGTTAAATCTGTGAAACTGATCTTGAGGTGTTTTATTTATTTATTTTTTCTATCAAGACTAAAATTTCCCAGAGGGGTACCGAG SEQUENCE_INCLUDED_REGION=0,126 P3_COMMENT=CL is AAGCAACCTCACCTCTACCA P3_COMMENT=CR is GTAGTCATTTCCAGGATCATCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.698290 PRIMER_LEFT_0_PENALTY=2.149011 PRIMER_RIGHT_0_PENALTY=0.549279 PRIMER_INTERNAL_0_PENALTY=3.018344 PRIMER_LEFT_0_SEQUENCE=AAAGCAACCTCACCTCTACCA PRIMER_RIGHT_0_SEQUENCE=CATTTCCAGGATCATCGACA PRIMER_INTERNAL_0_SEQUENCE=GGTGCTCACTGTCTGAATTATCC PRIMER_LEFT_0=14,21 PRIMER_RIGHT_0=109,20 PRIMER_INTERNAL_0=39,23 PRIMER_LEFT_0_TM=58.851 PRIMER_RIGHT_0_TM=59.451 PRIMER_INTERNAL_0_TM=60.018 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=47.826 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=96 PRIMER_PAIR_1_PENALTY=3.063067 PRIMER_LEFT_1_PENALTY=2.149011 PRIMER_RIGHT_1_PENALTY=0.914056 PRIMER_INTERNAL_1_PENALTY=3.018344 PRIMER_LEFT_1_SEQUENCE=AAAGCAACCTCACCTCTACCA PRIMER_RIGHT_1_SEQUENCE=CCAGGATCATCGACAAACTG PRIMER_INTERNAL_1_SEQUENCE=GGTGCTCACTGTCTGAATTATCC PRIMER_LEFT_1=14,21 PRIMER_RIGHT_1=104,20 PRIMER_INTERNAL_1=39,23 PRIMER_LEFT_1_TM=58.851 PRIMER_RIGHT_1_TM=59.086 PRIMER_INTERNAL_1_TM=60.018 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=47.826 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=3.099713 PRIMER_LEFT_2_PENALTY=2.149011 PRIMER_RIGHT_2_PENALTY=0.950702 PRIMER_INTERNAL_2_PENALTY=3.018344 PRIMER_LEFT_2_SEQUENCE=AAAGCAACCTCACCTCTACCA PRIMER_RIGHT_2_SEQUENCE=TTTCCAGGATCATCGACAAA PRIMER_INTERNAL_2_SEQUENCE=GGTGCTCACTGTCTGAATTATCC PRIMER_LEFT_2=14,21 PRIMER_RIGHT_2=107,20 PRIMER_INTERNAL_2=39,23 PRIMER_LEFT_2_TM=58.851 PRIMER_RIGHT_2_TM=59.049 PRIMER_INTERNAL_2_TM=60.018 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=47.826 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=94 PRIMER_PAIR_3_PENALTY=3.162285 PRIMER_LEFT_3_PENALTY=2.613006 PRIMER_RIGHT_3_PENALTY=0.549279 PRIMER_INTERNAL_3_PENALTY=3.018344 PRIMER_LEFT_3_SEQUENCE=AAGCAACCTCACCTCTACCA PRIMER_RIGHT_3_SEQUENCE=CATTTCCAGGATCATCGACA PRIMER_INTERNAL_3_SEQUENCE=GGTGCTCACTGTCTGAATTATCC PRIMER_LEFT_3=15,20 PRIMER_RIGHT_3=109,20 PRIMER_INTERNAL_3=39,23 PRIMER_LEFT_3_TM=57.387 PRIMER_RIGHT_3_TM=59.451 PRIMER_INTERNAL_3_TM=60.018 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=47.826 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=8.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=95 PRIMER_PAIR_4_PENALTY=3.167523 PRIMER_LEFT_4_PENALTY=2.149011 PRIMER_RIGHT_4_PENALTY=1.018512 PRIMER_INTERNAL_4_PENALTY=3.018344 PRIMER_LEFT_4_SEQUENCE=AAAGCAACCTCACCTCTACCA PRIMER_RIGHT_4_SEQUENCE=CCAGGATCATCGACAAACTGT PRIMER_INTERNAL_4_SEQUENCE=GGTGCTCACTGTCTGAATTATCC PRIMER_LEFT_4=14,21 PRIMER_RIGHT_4=104,21 PRIMER_INTERNAL_4=39,23 PRIMER_LEFT_4_TM=58.851 PRIMER_RIGHT_4_TM=59.981 PRIMER_INTERNAL_4_TM=60.018 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_INTERNAL_4_GC_PERCENT=47.826 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=6.1000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH481 SEQUENCE_TEMPLATE=AATGAAAGAAGATCTGAGAGACATGTGATCTTATTATGTTCGTTGTAAATACACAGAATACTGGAAACAACATAAAGCCAAATAAAAAATGATCATTTTTATAATGTATGGAATAACTGTGCAATTTTTCTAGTACAATAAAATAATTTAAATCTATATTGTATGATTGCATGGCAGTTCACGATATATTAATAAAAA SEQUENCE_INCLUDED_REGION=0,198 P3_COMMENT=CL is GAGACATGTGATCTTATTATGTTCG P3_COMMENT=CR is ATATATCGTGAACTGCCATGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=11.414870 PRIMER_LEFT_0_PENALTY=5.064245 PRIMER_RIGHT_0_PENALTY=6.350625 PRIMER_INTERNAL_0_PENALTY=6.911248 PRIMER_LEFT_0_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG PRIMER_RIGHT_0_SEQUENCE=TCATTTTTTATTTGGCTTTATGTTG PRIMER_INTERNAL_0_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=91,25 PRIMER_INTERNAL_0=39,26 PRIMER_LEFT_0_TM=59.936 PRIMER_RIGHT_0_TM=58.649 PRIMER_INTERNAL_0_TM=59.089 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=24.000 PRIMER_INTERNAL_0_GC_PERCENT=34.615 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=90 PRIMER_PAIR_1_PENALTY=11.454780 PRIMER_LEFT_1_PENALTY=5.064245 PRIMER_RIGHT_1_PENALTY=6.390536 PRIMER_INTERNAL_1_PENALTY=6.911248 PRIMER_LEFT_1_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG PRIMER_RIGHT_1_SEQUENCE=TGATCATTTTTTATTTGGCTTTATG PRIMER_INTERNAL_1_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA PRIMER_LEFT_1=2,25 PRIMER_RIGHT_1=94,25 PRIMER_INTERNAL_1=39,26 PRIMER_LEFT_1_TM=59.936 PRIMER_RIGHT_1_TM=58.609 PRIMER_INTERNAL_1_TM=59.089 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=24.000 PRIMER_INTERNAL_1_GC_PERCENT=34.615 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=6.2000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=93 PRIMER_PAIR_2_PENALTY=11.509976 PRIMER_LEFT_2_PENALTY=5.064245 PRIMER_RIGHT_2_PENALTY=6.445731 PRIMER_INTERNAL_2_PENALTY=6.911248 PRIMER_LEFT_2_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG PRIMER_RIGHT_2_SEQUENCE=AAATGATCATTTTTTATTTGGCTTT PRIMER_INTERNAL_2_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA PRIMER_LEFT_2=2,25 PRIMER_RIGHT_2=97,25 PRIMER_INTERNAL_2=39,26 PRIMER_LEFT_2_TM=59.936 PRIMER_RIGHT_2_TM=58.554 PRIMER_INTERNAL_2_TM=59.089 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=20.000 PRIMER_INTERNAL_2_GC_PERCENT=34.615 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=12.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=11.661126 PRIMER_LEFT_3_PENALTY=5.064245 PRIMER_RIGHT_3_PENALTY=6.596881 PRIMER_INTERNAL_3_PENALTY=6.911248 PRIMER_LEFT_3_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG PRIMER_RIGHT_3_SEQUENCE=AATGATCATTTTTTATTTGGCTTT PRIMER_INTERNAL_3_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA PRIMER_LEFT_3=2,25 PRIMER_RIGHT_3=96,24 PRIMER_INTERNAL_3=39,26 PRIMER_LEFT_3_TM=59.936 PRIMER_RIGHT_3_TM=57.403 PRIMER_INTERNAL_3_TM=59.089 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=20.833 PRIMER_INTERNAL_3_GC_PERCENT=34.615 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=10.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=95 PRIMER_PAIR_4_PENALTY=11.661126 PRIMER_LEFT_4_PENALTY=5.064245 PRIMER_RIGHT_4_PENALTY=6.596881 PRIMER_INTERNAL_4_PENALTY=6.911248 PRIMER_LEFT_4_SEQUENCE=TGAAAGAAGATCTGAGAGACATGTG PRIMER_RIGHT_4_SEQUENCE=AAATGATCATTTTTTATTTGGCTT PRIMER_INTERNAL_4_SEQUENCE=TCGTTGTAAATACACAGAATACTGGA PRIMER_LEFT_4=2,25 PRIMER_RIGHT_4=97,24 PRIMER_INTERNAL_4=39,26 PRIMER_LEFT_4_TM=59.936 PRIMER_RIGHT_4_TM=57.403 PRIMER_INTERNAL_4_TM=59.089 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=20.833 PRIMER_INTERNAL_4_GC_PERCENT=34.615 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=12.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=96 = SEQUENCE_ID=MH484 SEQUENCE_TEMPLATE=TCCATTAAAGAGACATAAGATCACATCACTTCTGTGCTCAAAACCCCACAATGACTCTCTATTTCCCAGAGGGAAAGCCAGTCTTTACCAGAGTCTACAAAGCCTACACACTCGATCCCTGTTGTTTCTTGACCTCATTTCTTACATCTTCTCCAGGNCCACTCCACTCCAGCCACACAGCCTCCTTGCTTTTCCTTGAAAACACCAGGCACTCACACACTGCGAGGCTTTGAAGTGGCTGTTCTCTCTGCCTGGAATATTTCAATACATGTATCTGCATAGGGGTACCGAGCTCGAATTCGTAATCATGG SEQUENCE_INCLUDED_REGION=0,162 P3_COMMENT=CL is CCATTAAAGAGACATAAGATCACA P3_COMMENT=CR is TGGAGAAGATGTAAGAAATGAGG P3_COMMENT=CM is CACTTCTGTGCTCAAAACCC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.039952 PRIMER_LEFT_0_PENALTY=0.361767 PRIMER_RIGHT_0_PENALTY=0.678186 PRIMER_INTERNAL_0_PENALTY=0.390581 PRIMER_LEFT_0_SEQUENCE=GCTCAAAACCCCACAATGAC PRIMER_RIGHT_0_SEQUENCE=CAGGGATCGAGTGTGTAGGC PRIMER_INTERNAL_0_SEQUENCE=TATTTCCCAGAGGGAAAGCC PRIMER_LEFT_0=35,20 PRIMER_RIGHT_0=120,20 PRIMER_INTERNAL_0=59,20 PRIMER_LEFT_0_TM=60.362 PRIMER_RIGHT_0_TM=60.678 PRIMER_INTERNAL_0_TM=60.391 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=9.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.3000 PRIMER_RIGHT_0_END_STABILITY=8.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=86 PRIMER_PAIR_1_PENALTY=1.195846 PRIMER_LEFT_1_PENALTY=0.361767 PRIMER_RIGHT_1_PENALTY=0.834079 PRIMER_INTERNAL_1_PENALTY=0.390581 PRIMER_LEFT_1_SEQUENCE=GCTCAAAACCCCACAATGAC PRIMER_RIGHT_1_SEQUENCE=GGGATCGAGTGTGTAGGCTT PRIMER_INTERNAL_1_SEQUENCE=TATTTCCCAGAGGGAAAGCC PRIMER_LEFT_1=35,20 PRIMER_RIGHT_1=118,20 PRIMER_INTERNAL_1=59,20 PRIMER_LEFT_1_TM=60.362 PRIMER_RIGHT_1_TM=59.166 PRIMER_INTERNAL_1_TM=60.391 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=9.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.3000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=84 PRIMER_PAIR_2_PENALTY=1.382055 PRIMER_LEFT_2_PENALTY=0.361767 PRIMER_RIGHT_2_PENALTY=1.020289 PRIMER_INTERNAL_2_PENALTY=0.390581 PRIMER_LEFT_2_SEQUENCE=GCTCAAAACCCCACAATGAC PRIMER_RIGHT_2_SEQUENCE=AAACAACAGGGATCGAGTGTG PRIMER_INTERNAL_2_SEQUENCE=TATTTCCCAGAGGGAAAGCC PRIMER_LEFT_2=35,20 PRIMER_RIGHT_2=126,21 PRIMER_INTERNAL_2=59,20 PRIMER_LEFT_2_TM=60.362 PRIMER_RIGHT_2_TM=60.020 PRIMER_INTERNAL_2_TM=60.391 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=9.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.3000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=92 PRIMER_PAIR_3_PENALTY=1.491207 PRIMER_LEFT_3_PENALTY=0.813021 PRIMER_RIGHT_3_PENALTY=0.678186 PRIMER_INTERNAL_3_PENALTY=0.390581 PRIMER_LEFT_3_SEQUENCE=ACTTCTGTGCTCAAAACCCC PRIMER_RIGHT_3_SEQUENCE=CAGGGATCGAGTGTGTAGGC PRIMER_INTERNAL_3_SEQUENCE=TATTTCCCAGAGGGAAAGCC PRIMER_LEFT_3=27,20 PRIMER_RIGHT_3=120,20 PRIMER_INTERNAL_3=59,20 PRIMER_LEFT_3_TM=59.187 PRIMER_RIGHT_3_TM=60.678 PRIMER_INTERNAL_3_TM=60.391 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=9.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=10.6000 PRIMER_RIGHT_3_END_STABILITY=8.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=1.507911 PRIMER_LEFT_4_PENALTY=0.361767 PRIMER_RIGHT_4_PENALTY=1.146144 PRIMER_INTERNAL_4_PENALTY=0.390581 PRIMER_LEFT_4_SEQUENCE=GCTCAAAACCCCACAATGAC PRIMER_RIGHT_4_SEQUENCE=AGGGATCGAGTGTGTAGGCTT PRIMER_INTERNAL_4_SEQUENCE=TATTTCCCAGAGGGAAAGCC PRIMER_LEFT_4=35,20 PRIMER_RIGHT_4=119,21 PRIMER_INTERNAL_4=59,20 PRIMER_LEFT_4_TM=60.362 PRIMER_RIGHT_4_TM=60.146 PRIMER_INTERNAL_4_TM=60.391 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=9.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.3000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=85 = SEQUENCE_ID=MH486 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCAAAAAACTTGATTTGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=36,15 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH488 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAGACATTGTTCTTACCCTAGATCATTGTGATTTACAGAGAAAGAGCAATATAAGATCAATTGTACAAAACTCAAATGTTAGGAATTTCTCC SEQUENCE_INCLUDED_REGION=36,94 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=12.602333 PRIMER_LEFT_0_PENALTY=5.889990 PRIMER_RIGHT_0_PENALTY=6.712343 PRIMER_INTERNAL_0_PENALTY=8.906203 PRIMER_LEFT_0_SEQUENCE=TTGTTCTTACCCTAGATCATTGTGA PRIMER_RIGHT_0_SEQUENCE=GGAGAAATTCCTAACATTTGAGTTT PRIMER_INTERNAL_0_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA PRIMER_LEFT_0=44,25 PRIMER_RIGHT_0=129,25 PRIMER_INTERNAL_0=72,26 PRIMER_LEFT_0_TM=59.110 PRIMER_RIGHT_0_TM=58.288 PRIMER_INTERNAL_0_TM=57.094 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_INTERNAL_0_GC_PERCENT=30.769 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=86 PRIMER_PAIR_1_PENALTY=12.832894 PRIMER_LEFT_1_PENALTY=5.889990 PRIMER_RIGHT_1_PENALTY=6.942904 PRIMER_INTERNAL_1_PENALTY=8.906203 PRIMER_LEFT_1_SEQUENCE=TTGTTCTTACCCTAGATCATTGTGA PRIMER_RIGHT_1_SEQUENCE=GGAGAAATTCCTAACATTTGAGTT PRIMER_INTERNAL_1_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA PRIMER_LEFT_1=44,25 PRIMER_RIGHT_1=129,24 PRIMER_INTERNAL_1=72,26 PRIMER_LEFT_1_TM=59.110 PRIMER_RIGHT_1_TM=57.057 PRIMER_INTERNAL_1_TM=57.094 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=33.333 PRIMER_INTERNAL_1_GC_PERCENT=30.769 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=86 PRIMER_PAIR_2_PENALTY=12.884753 PRIMER_LEFT_2_PENALTY=6.172410 PRIMER_RIGHT_2_PENALTY=6.712343 PRIMER_INTERNAL_2_PENALTY=8.906203 PRIMER_LEFT_2_SEQUENCE=TGTTCTTACCCTAGATCATTGTGA PRIMER_RIGHT_2_SEQUENCE=GGAGAAATTCCTAACATTTGAGTTT PRIMER_INTERNAL_2_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA PRIMER_LEFT_2=45,24 PRIMER_RIGHT_2=129,25 PRIMER_INTERNAL_2=72,26 PRIMER_LEFT_2_TM=57.828 PRIMER_RIGHT_2_TM=58.288 PRIMER_INTERNAL_2_TM=57.094 PRIMER_LEFT_2_GC_PERCENT=37.500 PRIMER_RIGHT_2_GC_PERCENT=32.000 PRIMER_INTERNAL_2_GC_PERCENT=30.769 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=85 PRIMER_PAIR_3_PENALTY=13.115314 PRIMER_LEFT_3_PENALTY=6.172410 PRIMER_RIGHT_3_PENALTY=6.942904 PRIMER_INTERNAL_3_PENALTY=8.906203 PRIMER_LEFT_3_SEQUENCE=TGTTCTTACCCTAGATCATTGTGA PRIMER_RIGHT_3_SEQUENCE=GGAGAAATTCCTAACATTTGAGTT PRIMER_INTERNAL_3_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA PRIMER_LEFT_3=45,24 PRIMER_RIGHT_3=129,24 PRIMER_INTERNAL_3=72,26 PRIMER_LEFT_3_TM=57.828 PRIMER_RIGHT_3_TM=57.057 PRIMER_INTERNAL_3_TM=57.094 PRIMER_LEFT_3_GC_PERCENT=37.500 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=30.769 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=85 PRIMER_PAIR_4_PENALTY=13.302043 PRIMER_LEFT_4_PENALTY=6.589700 PRIMER_RIGHT_4_PENALTY=6.712343 PRIMER_INTERNAL_4_PENALTY=8.906203 PRIMER_LEFT_4_SEQUENCE=TTGTTCTTACCCTAGATCATTGTG PRIMER_RIGHT_4_SEQUENCE=GGAGAAATTCCTAACATTTGAGTTT PRIMER_INTERNAL_4_SEQUENCE=ACAGAGAAAGAGCAATATAAGATCAA PRIMER_LEFT_4=44,24 PRIMER_RIGHT_4=129,25 PRIMER_INTERNAL_4=72,26 PRIMER_LEFT_4_TM=57.410 PRIMER_RIGHT_4_TM=58.288 PRIMER_INTERNAL_4_TM=57.094 PRIMER_LEFT_4_GC_PERCENT=37.500 PRIMER_RIGHT_4_GC_PERCENT=32.000 PRIMER_INTERNAL_4_GC_PERCENT=30.769 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH490 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAATTTCAAATTATGCATACTATATGCAGACAACTTTGTAAACAAATAAAAAACAGGAAAAGATGGGTAAGTTATACATTAAAATGTTAACAATAATTATCCTTGAGTNNTNGGACCTTGTAATTTTTACGTTTATATATTTNCTTAATATTGAAAATTCATACTCTTGCAGTNNAAACTATGTTTTGAAGCCCTGTCACATAGATGAGAATTAGACTTATTTTCTATGACTCTGGAAGCAGAATAAG SEQUENCE_INCLUDED_REGION=36,250 P3_COMMENT=CL is ATAAAAAACAGGAAAAGATGGG P3_COMMENT=CR is TTCTCATCTATGTGACAGGGC P3_COMMENT=CM is ACCCATCTTTTCCTGTTTTTTAT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=7.436790 PRIMER_LEFT_0_PENALTY=7.158805 PRIMER_RIGHT_0_PENALTY=0.277985 PRIMER_INTERNAL_0_PENALTY=8.721666 PRIMER_LEFT_0_SEQUENCE=CAGGAAAAGATGGGTAAGTTATACA PRIMER_RIGHT_0_SEQUENCE=TGTGACAGGGCTTCAAAACA PRIMER_INTERNAL_0_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG PRIMER_LEFT_0=91,25 PRIMER_RIGHT_0=238,20 PRIMER_INTERNAL_0=184,26 PRIMER_LEFT_0_TM=57.841 PRIMER_RIGHT_0_TM=60.278 PRIMER_INTERNAL_0_TM=57.278 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=26.923 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=5.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=148 PRIMER_PAIR_1_PENALTY=7.980162 PRIMER_LEFT_1_PENALTY=5.619366 PRIMER_RIGHT_1_PENALTY=2.360796 PRIMER_INTERNAL_1_PENALTY=8.721666 PRIMER_LEFT_1_SEQUENCE=AAACAGGAAAAGATGGGTAAGTT PRIMER_RIGHT_1_SEQUENCE=GTGACAGGGCTTCAAAACAT PRIMER_INTERNAL_1_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG PRIMER_LEFT_1=88,23 PRIMER_RIGHT_1=237,20 PRIMER_INTERNAL_1=184,26 PRIMER_LEFT_1_TM=57.381 PRIMER_RIGHT_1_TM=57.639 PRIMER_INTERNAL_1_TM=57.278 PRIMER_LEFT_1_GC_PERCENT=34.783 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=26.923 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=150 PRIMER_PAIR_2_PENALTY=8.435604 PRIMER_LEFT_2_PENALTY=5.387193 PRIMER_RIGHT_2_PENALTY=3.048411 PRIMER_INTERNAL_2_PENALTY=8.721666 PRIMER_LEFT_2_SEQUENCE=AAAACAGGAAAAGATGGGTAAGTT PRIMER_RIGHT_2_SEQUENCE=TGACAGGGCTTCAAAACATAGTT PRIMER_INTERNAL_2_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG PRIMER_LEFT_2=87,24 PRIMER_RIGHT_2=236,23 PRIMER_INTERNAL_2=184,26 PRIMER_LEFT_2_TM=58.613 PRIMER_RIGHT_2_TM=60.048 PRIMER_INTERNAL_2_TM=57.278 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=39.130 PRIMER_INTERNAL_2_GC_PERCENT=26.923 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=5.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=150 PRIMER_PAIR_3_PENALTY=8.494563 PRIMER_LEFT_3_PENALTY=5.387193 PRIMER_RIGHT_3_PENALTY=3.107370 PRIMER_INTERNAL_3_PENALTY=8.721666 PRIMER_LEFT_3_SEQUENCE=AAAACAGGAAAAGATGGGTAAGTT PRIMER_RIGHT_3_SEQUENCE=TGACAGGGCTTCAAAACATAG PRIMER_INTERNAL_3_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG PRIMER_LEFT_3=87,24 PRIMER_RIGHT_3=236,21 PRIMER_INTERNAL_3=184,26 PRIMER_LEFT_3_TM=58.613 PRIMER_RIGHT_3_TM=57.893 PRIMER_INTERNAL_3_TM=57.278 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_INTERNAL_3_GC_PERCENT=26.923 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=5.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=150 PRIMER_PAIR_4_PENALTY=8.619905 PRIMER_LEFT_4_PENALTY=5.387193 PRIMER_RIGHT_4_PENALTY=3.232712 PRIMER_INTERNAL_4_PENALTY=8.721666 PRIMER_LEFT_4_SEQUENCE=AAAACAGGAAAAGATGGGTAAGTT PRIMER_RIGHT_4_SEQUENCE=TGACAGGGCTTCAAAACATAGT PRIMER_INTERNAL_4_SEQUENCE=AATATTGAAAATTCATACTCTTGCAG PRIMER_LEFT_4=87,24 PRIMER_RIGHT_4=236,22 PRIMER_INTERNAL_4=184,26 PRIMER_LEFT_4_TM=58.613 PRIMER_RIGHT_4_TM=58.767 PRIMER_INTERNAL_4_TM=57.278 PRIMER_LEFT_4_GC_PERCENT=33.333 PRIMER_RIGHT_4_GC_PERCENT=40.909 PRIMER_INTERNAL_4_GC_PERCENT=26.923 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=5.3000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=150 = SEQUENCE_ID=MH504 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAACCTGGTTTGAAATTTGGGGGTCTGAGCCTCCACTGTNGTNTTACCTAGCAGGCTTCCCGGACTTAGTCTTACATGCACATGCACACCGTAAAAAAGAGACCAAAGAAAAATCTGCAAGANTTGCTCAAATAACACAAGACGGGGATGAACTGGATCTACAGGTCTAGAGTNNTTCAGAACTAAAGACGCCTGTCACACGGCTTAAGCAAGAGCAGGAAGGGGCTGCCAGGCCTCTGGGAAGACCTCCCTGGGGAGNGAGCAGGCAGACAAATGGCCCGGATAGAGATCCCCCGNTTACGTGATGTTTTCCTTCCTAAAGATAGCGC SEQUENCE_INCLUDED_REGION=36,331 P3_COMMENT=CL is AACCTGGTTTGAAATTTGGG P3_COMMENT=CR is TTAAGCCGTGTGACAGGC P3_COMMENT=CM is TTACCTAGCAGGCTTCCCG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.302471 PRIMER_LEFT_0_PENALTY=0.050165 PRIMER_RIGHT_0_PENALTY=0.252306 PRIMER_INTERNAL_0_PENALTY=1.230410 PRIMER_LEFT_0_SEQUENCE=TGTCACACGGCTTAAGCAAG PRIMER_RIGHT_0_SEQUENCE=GATCTCTATCCGGGCCATTT PRIMER_INTERNAL_0_SEQUENCE=CTCTGGGAAGACCTCCCTG PRIMER_LEFT_0=231,20 PRIMER_RIGHT_0=328,20 PRIMER_INTERNAL_0=272,19 PRIMER_LEFT_0_TM=60.050 PRIMER_RIGHT_0_TM=60.252 PRIMER_INTERNAL_0_TM=59.770 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=63.158 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=7.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=98 PRIMER_PAIR_1_PENALTY=0.302471 PRIMER_LEFT_1_PENALTY=0.050165 PRIMER_RIGHT_1_PENALTY=0.252306 PRIMER_INTERNAL_1_PENALTY=1.230410 PRIMER_LEFT_1_SEQUENCE=CTGTCACACGGCTTAAGCAA PRIMER_RIGHT_1_SEQUENCE=GATCTCTATCCGGGCCATTT PRIMER_INTERNAL_1_SEQUENCE=CTCTGGGAAGACCTCCCTG PRIMER_LEFT_1=230,20 PRIMER_RIGHT_1=328,20 PRIMER_INTERNAL_1=272,19 PRIMER_LEFT_1_TM=60.050 PRIMER_RIGHT_1_TM=60.252 PRIMER_INTERNAL_1_TM=59.770 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=63.158 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=7.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=7.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=0.443054 PRIMER_LEFT_2_PENALTY=0.173087 PRIMER_RIGHT_2_PENALTY=0.269968 PRIMER_INTERNAL_2_PENALTY=2.052187 PRIMER_LEFT_2_SEQUENCE=AACACAAGACGGGGATGAAC PRIMER_RIGHT_2_SEQUENCE=TTCCTGCTCTTGCTTAAGCC PRIMER_INTERNAL_2_SEQUENCE=TCAGAACTAAAGACGCCTGTCA PRIMER_LEFT_2=171,20 PRIMER_RIGHT_2=258,20 PRIMER_INTERNAL_2=214,22 PRIMER_LEFT_2_TM=59.827 PRIMER_RIGHT_2_TM=59.730 PRIMER_INTERNAL_2_TM=60.052 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=88 PRIMER_PAIR_3_PENALTY=0.460268 PRIMER_LEFT_3_PENALTY=0.287182 PRIMER_RIGHT_3_PENALTY=0.173087 PRIMER_INTERNAL_3_PENALTY=0.566194 PRIMER_LEFT_3_SEQUENCE=GGCTTCCCGGACTTAGTCTT PRIMER_RIGHT_3_SEQUENCE=GTTCATCCCCGTCTTGTGTT PRIMER_INTERNAL_3_SEQUENCE=GCACATGCACACCGTAAAAA PRIMER_LEFT_3=91,20 PRIMER_RIGHT_3=190,20 PRIMER_INTERNAL_3=115,20 PRIMER_LEFT_3_TM=59.713 PRIMER_RIGHT_3_TM=59.827 PRIMER_INTERNAL_3_TM=60.566 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=0.522274 PRIMER_LEFT_4_PENALTY=0.269968 PRIMER_RIGHT_4_PENALTY=0.252306 PRIMER_INTERNAL_4_PENALTY=1.230410 PRIMER_LEFT_4_SEQUENCE=GGCTTAAGCAAGAGCAGGAA PRIMER_RIGHT_4_SEQUENCE=GATCTCTATCCGGGCCATTT PRIMER_INTERNAL_4_SEQUENCE=CTCTGGGAAGACCTCCCTG PRIMER_LEFT_4=239,20 PRIMER_RIGHT_4=328,20 PRIMER_INTERNAL_4=272,19 PRIMER_LEFT_4_TM=59.730 PRIMER_RIGHT_4_TM=60.252 PRIMER_INTERNAL_4_TM=59.770 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=63.158 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=7.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=7.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=90 = SEQUENCE_ID=MH509 SEQUENCE_TEMPLATE=GCTTGCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGCTCCTGGGAAGGAGGCTGAGACTCACATTGTNAGCTTTCACTCACTAAAGAGAGTCTTGCTTGAGGGGCTTGGACAAACAGGAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=35,86 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.561777 PRIMER_LEFT_0_PENALTY=0.921348 PRIMER_RIGHT_0_PENALTY=1.640429 PRIMER_INTERNAL_0_PENALTY=6.525910 PRIMER_LEFT_0_SEQUENCE=CTGGGAAGGAGGCTGAGACT PRIMER_RIGHT_0_SEQUENCE=CTGTTTGTCCAAGCCCCTC PRIMER_INTERNAL_0_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC PRIMER_LEFT_0=41,20 PRIMER_RIGHT_0=118,19 PRIMER_INTERNAL_0=73,25 PRIMER_LEFT_0_TM=60.921 PRIMER_RIGHT_0_TM=60.640 PRIMER_INTERNAL_0_TM=58.474 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.1000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 PRIMER_PAIR_1_PENALTY=2.663352 PRIMER_LEFT_1_PENALTY=1.022923 PRIMER_RIGHT_1_PENALTY=1.640429 PRIMER_INTERNAL_1_PENALTY=6.525910 PRIMER_LEFT_1_SEQUENCE=GGAAGGAGGCTGAGACTCAC PRIMER_RIGHT_1_SEQUENCE=CTGTTTGTCCAAGCCCCTC PRIMER_INTERNAL_1_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC PRIMER_LEFT_1=44,20 PRIMER_RIGHT_1=118,19 PRIMER_INTERNAL_1=73,25 PRIMER_LEFT_1_TM=58.977 PRIMER_RIGHT_1_TM=60.640 PRIMER_INTERNAL_1_TM=58.474 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=2.711523 PRIMER_LEFT_2_PENALTY=1.071094 PRIMER_RIGHT_2_PENALTY=1.640429 PRIMER_INTERNAL_2_PENALTY=6.525910 PRIMER_LEFT_2_SEQUENCE=TGGGAAGGAGGCTGAGACT PRIMER_RIGHT_2_SEQUENCE=CTGTTTGTCCAAGCCCCTC PRIMER_INTERNAL_2_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC PRIMER_LEFT_2=42,19 PRIMER_RIGHT_2=118,19 PRIMER_INTERNAL_2=73,25 PRIMER_LEFT_2_TM=59.929 PRIMER_RIGHT_2_TM=60.640 PRIMER_INTERNAL_2_TM=58.474 PRIMER_LEFT_2_GC_PERCENT=57.895 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.1000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=2.711523 PRIMER_LEFT_3_PENALTY=1.071094 PRIMER_RIGHT_3_PENALTY=1.640429 PRIMER_INTERNAL_3_PENALTY=6.525910 PRIMER_LEFT_3_SEQUENCE=CTGGGAAGGAGGCTGAGAC PRIMER_RIGHT_3_SEQUENCE=CTGTTTGTCCAAGCCCCTC PRIMER_INTERNAL_3_SEQUENCE=TTTCACTCACTAAAGAGAGTCTTGC PRIMER_LEFT_3=41,19 PRIMER_RIGHT_3=118,19 PRIMER_INTERNAL_3=73,25 PRIMER_LEFT_3_TM=59.929 PRIMER_RIGHT_3_TM=60.640 PRIMER_INTERNAL_3_TM=58.474 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=57.895 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.1000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=5.00 PRIMER_PAIR_3_PRODUCT_SIZE=78 PRIMER_PAIR_4_PENALTY=2.862491 PRIMER_LEFT_4_PENALTY=0.921348 PRIMER_RIGHT_4_PENALTY=1.941143 PRIMER_INTERNAL_4_PENALTY=6.645031 PRIMER_LEFT_4_SEQUENCE=CTGGGAAGGAGGCTGAGACT PRIMER_RIGHT_4_SEQUENCE=GTTTGTCCAAGCCCCTCAAG PRIMER_INTERNAL_4_SEQUENCE=GCTTTCACTCACTAAAGAGAGTCTTG PRIMER_LEFT_4=41,20 PRIMER_RIGHT_4=116,20 PRIMER_INTERNAL_4=71,26 PRIMER_LEFT_4_TM=60.921 PRIMER_RIGHT_4_TM=61.941 PRIMER_INTERNAL_4_TM=59.355 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=42.308 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.1000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH512 SEQUENCE_TEMPLATE=CCTGCATGTGGCACCCACCTGAGGCCAGAGACATCTGGAACTCTAAGATTGGGCCCCACAGGAGGATGCTGTGTGGGTTCTGTGGATCCCAGCCACTCCAAAGAGGACACTCTCGGCAGAGGTTCT SEQUENCE_INCLUDED_REGION=0,126 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.103757 PRIMER_LEFT_0_PENALTY=0.096820 PRIMER_RIGHT_0_PENALTY=0.006937 PRIMER_INTERNAL_0_PENALTY=0.119809 PRIMER_LEFT_0_SEQUENCE=GAACTCTAAGATTGGGCCCC PRIMER_RIGHT_0_SEQUENCE=GAACCTCTGCCGAGAGTGTC PRIMER_INTERNAL_0_SEQUENCE=GAGGATGCTGTGTGGGTTCT PRIMER_LEFT_0=37,20 PRIMER_RIGHT_0=124,20 PRIMER_INTERNAL_0=61,20 PRIMER_LEFT_0_TM=59.903 PRIMER_RIGHT_0_TM=59.993 PRIMER_INTERNAL_0_TM=60.120 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 PRIMER_PAIR_1_PENALTY=0.103757 PRIMER_LEFT_1_PENALTY=0.096820 PRIMER_RIGHT_1_PENALTY=0.006937 PRIMER_INTERNAL_1_PENALTY=0.119809 PRIMER_LEFT_1_SEQUENCE=GGAACTCTAAGATTGGGCCC PRIMER_RIGHT_1_SEQUENCE=GAACCTCTGCCGAGAGTGTC PRIMER_INTERNAL_1_SEQUENCE=GAGGATGCTGTGTGGGTTCT PRIMER_LEFT_1=36,20 PRIMER_RIGHT_1=124,20 PRIMER_INTERNAL_1=61,20 PRIMER_LEFT_1_TM=59.903 PRIMER_RIGHT_1_TM=59.993 PRIMER_INTERNAL_1_TM=60.120 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=12.4000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=89 PRIMER_PAIR_2_PENALTY=0.232151 PRIMER_LEFT_2_PENALTY=0.096820 PRIMER_RIGHT_2_PENALTY=0.135331 PRIMER_INTERNAL_2_PENALTY=0.119809 PRIMER_LEFT_2_SEQUENCE=GAACTCTAAGATTGGGCCCC PRIMER_RIGHT_2_SEQUENCE=TCTGCCGAGAGTGTCCTCTT PRIMER_INTERNAL_2_SEQUENCE=GAGGATGCTGTGTGGGTTCT PRIMER_LEFT_2=37,20 PRIMER_RIGHT_2=119,20 PRIMER_INTERNAL_2=61,20 PRIMER_LEFT_2_TM=59.903 PRIMER_RIGHT_2_TM=60.135 PRIMER_INTERNAL_2_TM=60.120 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=12.4000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=0.232151 PRIMER_LEFT_3_PENALTY=0.096820 PRIMER_RIGHT_3_PENALTY=0.135331 PRIMER_INTERNAL_3_PENALTY=0.119809 PRIMER_LEFT_3_SEQUENCE=GGAACTCTAAGATTGGGCCC PRIMER_RIGHT_3_SEQUENCE=TCTGCCGAGAGTGTCCTCTT PRIMER_INTERNAL_3_SEQUENCE=GAGGATGCTGTGTGGGTTCT PRIMER_LEFT_3=36,20 PRIMER_RIGHT_3=119,20 PRIMER_INTERNAL_3=61,20 PRIMER_LEFT_3_TM=59.903 PRIMER_RIGHT_3_TM=60.135 PRIMER_INTERNAL_3_TM=60.120 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=12.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=84 PRIMER_PAIR_4_PENALTY=0.334471 PRIMER_LEFT_4_PENALTY=0.327533 PRIMER_RIGHT_4_PENALTY=0.006937 PRIMER_INTERNAL_4_PENALTY=0.119809 PRIMER_LEFT_4_SEQUENCE=ACTCTAAGATTGGGCCCCAC PRIMER_RIGHT_4_SEQUENCE=GAACCTCTGCCGAGAGTGTC PRIMER_INTERNAL_4_SEQUENCE=GAGGATGCTGTGTGGGTTCT PRIMER_LEFT_4=39,20 PRIMER_RIGHT_4=124,20 PRIMER_INTERNAL_4=61,20 PRIMER_LEFT_4_TM=60.328 PRIMER_RIGHT_4_TM=59.993 PRIMER_INTERNAL_4_TM=60.120 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=6.1000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH516 SEQUENCE_TEMPLATE=TAGGGTCCTTCTCCCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTTNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG SEQUENCE_INCLUDED_REGION=0,17 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH519 SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCGTGGCACCCGGCAGCCTCCCAGGAGACAGCCAGGCAGGAGTGATGATCAGGATTGTGTTCAGTTTTCAGCATGTTTCCATGTACCCAAAAGTTAAGAGTCCCATCTACCTCTATAGTAAAACAAAATGATAGTAAATACATTCCATAAAAATGTTTTTCAAGCCATCAGTTTTGTTTCCGGCCTTATCTGTCAGTATTCCTTTCAGGCACATTCTGAGATTCACAACTGTGTTCTTTAAACAGCCAAAATTCCCTCTTCTCCAGCATGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAG SEQUENCE_INCLUDED_REGION=36,237 P3_COMMENT=CL is TTCAGTTTTCAGCATGTTTCC P3_COMMENT=CR is CTCAGAATGTGCCTGAAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.262193 PRIMER_LEFT_0_PENALTY=2.513402 PRIMER_RIGHT_0_PENALTY=0.748791 PRIMER_INTERNAL_0_PENALTY=0.222782 PRIMER_LEFT_0_SEQUENCE=AGCCTCCCAGGAGACAGC PRIMER_RIGHT_0_SEQUENCE=TTGGGTACATGGAAACATGC PRIMER_INTERNAL_0_SEQUENCE=AGGCAGGAGTGATGATCAGG PRIMER_LEFT_0=52,18 PRIMER_RIGHT_0=127,20 PRIMER_INTERNAL_0=71,20 PRIMER_LEFT_0_TM=60.513 PRIMER_RIGHT_0_TM=59.251 PRIMER_INTERNAL_0_TM=60.223 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=8.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=3.279658 PRIMER_LEFT_1_PENALTY=2.530866 PRIMER_RIGHT_1_PENALTY=0.748791 PRIMER_INTERNAL_1_PENALTY=0.222782 PRIMER_LEFT_1_SEQUENCE=CAGCCTCCCAGGAGACAG PRIMER_RIGHT_1_SEQUENCE=TTGGGTACATGGAAACATGC PRIMER_INTERNAL_1_SEQUENCE=AGGCAGGAGTGATGATCAGG PRIMER_LEFT_1=51,18 PRIMER_RIGHT_1=127,20 PRIMER_INTERNAL_1=71,20 PRIMER_LEFT_1_TM=59.469 PRIMER_RIGHT_1_TM=59.251 PRIMER_INTERNAL_1_TM=60.223 PRIMER_LEFT_1_GC_PERCENT=66.667 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=3.334690 PRIMER_LEFT_2_PENALTY=0.222782 PRIMER_RIGHT_2_PENALTY=3.111908 PRIMER_INTERNAL_2_PENALTY=1.692817 PRIMER_LEFT_2_SEQUENCE=AGGCAGGAGTGATGATCAGG PRIMER_RIGHT_2_SEQUENCE=AGATGGGACTCTTAACTTTTGGG PRIMER_INTERNAL_2_SEQUENCE=TCAGTTTTCAGCATGTTTCCA PRIMER_LEFT_2=71,20 PRIMER_RIGHT_2=145,23 PRIMER_INTERNAL_2=98,21 PRIMER_LEFT_2_TM=60.223 PRIMER_RIGHT_2_TM=59.888 PRIMER_INTERNAL_2_TM=59.307 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=43.478 PRIMER_INTERNAL_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=3.632149 PRIMER_LEFT_3_PENALTY=0.633068 PRIMER_RIGHT_3_PENALTY=2.999081 PRIMER_INTERNAL_3_PENALTY=1.692817 PRIMER_LEFT_3_SEQUENCE=CAGGCAGGAGTGATGATCAG PRIMER_RIGHT_3_SEQUENCE=GATGGGACTCTTAACTTTTGGG PRIMER_INTERNAL_3_SEQUENCE=TCAGTTTTCAGCATGTTTCCA PRIMER_LEFT_3=70,20 PRIMER_RIGHT_3=144,22 PRIMER_INTERNAL_3=98,21 PRIMER_LEFT_3_TM=59.367 PRIMER_RIGHT_3_TM=59.001 PRIMER_INTERNAL_3_TM=59.307 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.455 PRIMER_INTERNAL_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=3.663301 PRIMER_LEFT_4_PENALTY=2.513402 PRIMER_RIGHT_4_PENALTY=1.149900 PRIMER_INTERNAL_4_PENALTY=0.222782 PRIMER_LEFT_4_SEQUENCE=AGCCTCCCAGGAGACAGC PRIMER_RIGHT_4_SEQUENCE=TGGGTACATGGAAACATGCT PRIMER_INTERNAL_4_SEQUENCE=AGGCAGGAGTGATGATCAGG PRIMER_LEFT_4=52,18 PRIMER_RIGHT_4=126,20 PRIMER_INTERNAL_4=71,20 PRIMER_LEFT_4_TM=60.513 PRIMER_RIGHT_4_TM=58.850 PRIMER_INTERNAL_4_TM=60.223 PRIMER_LEFT_4_GC_PERCENT=66.667 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH521 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACTAAAAAGATATCCTCAAAGAATNTAATCTAGATCCTAGAATTTAGGATAATACTTAAAAATATGTATTTTAGATATACCTTCTCTACGTTATATTATTAAAATAATGTGTTTTCAATTTCCACATTGACAATAACTTTTCTGTAATAACTTGGTTTTTGAACCTATAACAAATACACATGAATATAGGGAGTTAATCATTTGGTAACCAATAAATGCTCCACAGACTTCCCATATTATAATAAACCTCAGGTAATATCTGATTCTTCAGAAATTCACCCA SEQUENCE_INCLUDED_REGION=33,284 P3_COMMENT=CL is AAATAATGTGTTTTCAATTTCCACA P3_COMMENT=CR is TGGGTGAATTTCTGAAGAATCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.091192 PRIMER_LEFT_0_PENALTY=2.637146 PRIMER_RIGHT_0_PENALTY=2.454047 PRIMER_INTERNAL_0_PENALTY=8.235014 PRIMER_LEFT_0_SEQUENCE=TTGGTAACCAATAAATGCTCCA PRIMER_RIGHT_0_SEQUENCE=TGGGTGAATTTCTGAAGAATCA PRIMER_INTERNAL_0_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG PRIMER_LEFT_0=236,22 PRIMER_RIGHT_0=316,22 PRIMER_INTERNAL_0=262,26 PRIMER_LEFT_0_TM=59.363 PRIMER_RIGHT_0_TM=59.546 PRIMER_INTERNAL_0_TM=57.765 PRIMER_LEFT_0_GC_PERCENT=36.364 PRIMER_RIGHT_0_GC_PERCENT=36.364 PRIMER_INTERNAL_0_GC_PERCENT=34.615 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=7.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=5.422938 PRIMER_LEFT_1_PENALTY=2.968891 PRIMER_RIGHT_1_PENALTY=2.454047 PRIMER_INTERNAL_1_PENALTY=8.235014 PRIMER_LEFT_1_SEQUENCE=TGGTAACCAATAAATGCTCCA PRIMER_RIGHT_1_SEQUENCE=TGGGTGAATTTCTGAAGAATCA PRIMER_INTERNAL_1_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG PRIMER_LEFT_1=237,21 PRIMER_RIGHT_1=316,22 PRIMER_INTERNAL_1=262,26 PRIMER_LEFT_1_TM=58.031 PRIMER_RIGHT_1_TM=59.546 PRIMER_INTERNAL_1_TM=57.765 PRIMER_LEFT_1_GC_PERCENT=38.095 PRIMER_RIGHT_1_GC_PERCENT=36.364 PRIMER_INTERNAL_1_GC_PERCENT=34.615 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=5.575532 PRIMER_LEFT_2_PENALTY=3.121486 PRIMER_RIGHT_2_PENALTY=2.454047 PRIMER_INTERNAL_2_PENALTY=8.235014 PRIMER_LEFT_2_SEQUENCE=TTGGTAACCAATAAATGCTCCAC PRIMER_RIGHT_2_SEQUENCE=TGGGTGAATTTCTGAAGAATCA PRIMER_INTERNAL_2_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG PRIMER_LEFT_2=236,23 PRIMER_RIGHT_2=316,22 PRIMER_INTERNAL_2=262,26 PRIMER_LEFT_2_TM=60.121 PRIMER_RIGHT_2_TM=59.546 PRIMER_INTERNAL_2_TM=57.765 PRIMER_LEFT_2_GC_PERCENT=39.130 PRIMER_RIGHT_2_GC_PERCENT=36.364 PRIMER_INTERNAL_2_GC_PERCENT=34.615 PRIMER_LEFT_2_SELF_ANY=9.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=10.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=7.00 PRIMER_INTERNAL_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=5.576335 PRIMER_LEFT_3_PENALTY=3.122288 PRIMER_RIGHT_3_PENALTY=2.454047 PRIMER_INTERNAL_3_PENALTY=8.235014 PRIMER_LEFT_3_SEQUENCE=GGTAACCAATAAATGCTCCACA PRIMER_RIGHT_3_SEQUENCE=TGGGTGAATTTCTGAAGAATCA PRIMER_INTERNAL_3_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG PRIMER_LEFT_3=238,22 PRIMER_RIGHT_3=316,22 PRIMER_INTERNAL_3=262,26 PRIMER_LEFT_3_TM=58.878 PRIMER_RIGHT_3_TM=59.546 PRIMER_INTERNAL_3_TM=57.765 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=36.364 PRIMER_INTERNAL_3_GC_PERCENT=34.615 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=10.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=7.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=5.576335 PRIMER_LEFT_4_PENALTY=3.122288 PRIMER_RIGHT_4_PENALTY=2.454047 PRIMER_INTERNAL_4_PENALTY=8.235014 PRIMER_LEFT_4_SEQUENCE=TGGTAACCAATAAATGCTCCAC PRIMER_RIGHT_4_SEQUENCE=TGGGTGAATTTCTGAAGAATCA PRIMER_INTERNAL_4_SEQUENCE=CTTCCCATATTATAATAAACCTCAGG PRIMER_LEFT_4=237,22 PRIMER_RIGHT_4=316,22 PRIMER_INTERNAL_4=262,26 PRIMER_LEFT_4_TM=58.878 PRIMER_RIGHT_4_TM=59.546 PRIMER_INTERNAL_4_TM=57.765 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=36.364 PRIMER_INTERNAL_4_GC_PERCENT=34.615 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=10.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=7.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH522 SEQUENCE_TEMPLATE=AGAATTTTAGTATTTTCTTTACTTTCAAATTTTCTGTCTCTCTATATTCATGGAATTACTAATGATAATTATTTAATACTGTCATCAGCGATCTCTATTATTCAGATCATTTATGTACCTATTCCTAAGATGTGTTTTTATGTTTCTTTTTACTGTCCAGTCATATGATCTTTTCTATTGACATGTCTTGTAATTTTNAAATNATTTTCTAGATAATGTTGTATAAATCATTCAAAGGCTTGAGATTATGACATCATCTGTGAGAGAATATTTACA SEQUENCE_INCLUDED_REGION=0,276 P3_COMMENT=CL is ATTTTCTTTACTTTCAAATTTTCTG P3_COMMENT=CR is ACAGATGATGTCATAATCTCAAGC P3_COMMENT=CM is ATTTAATACTGTCATCAGCGATCTC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=12.050740 PRIMER_LEFT_0_PENALTY=5.128157 PRIMER_RIGHT_0_PENALTY=6.922583 PRIMER_INTERNAL_0_PENALTY=9.885775 PRIMER_LEFT_0_SEQUENCE=TGTCATCAGCGATCTCTATTATTCA PRIMER_RIGHT_0_SEQUENCE=TGACTGGACAGTAAAAAGAAACAT PRIMER_INTERNAL_0_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT PRIMER_LEFT_0=79,25 PRIMER_RIGHT_0=162,24 PRIMER_INTERNAL_0=112,27 PRIMER_LEFT_0_TM=60.128 PRIMER_RIGHT_0_TM=57.077 PRIMER_INTERNAL_0_TM=57.114 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=33.333 PRIMER_INTERNAL_0_GC_PERCENT=29.630 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=84 PRIMER_PAIR_1_PENALTY=12.050740 PRIMER_LEFT_1_PENALTY=5.128157 PRIMER_RIGHT_1_PENALTY=6.922583 PRIMER_INTERNAL_1_PENALTY=9.885775 PRIMER_LEFT_1_SEQUENCE=TGTCATCAGCGATCTCTATTATTCA PRIMER_RIGHT_1_SEQUENCE=ATGACTGGACAGTAAAAAGAAACA PRIMER_INTERNAL_1_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT PRIMER_LEFT_1=79,25 PRIMER_RIGHT_1=163,24 PRIMER_INTERNAL_1=112,27 PRIMER_LEFT_1_TM=60.128 PRIMER_RIGHT_1_TM=57.077 PRIMER_INTERNAL_1_TM=57.114 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=33.333 PRIMER_INTERNAL_1_GC_PERCENT=29.630 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=85 PRIMER_PAIR_2_PENALTY=12.511201 PRIMER_LEFT_2_PENALTY=5.588618 PRIMER_RIGHT_2_PENALTY=6.922583 PRIMER_INTERNAL_2_PENALTY=9.885775 PRIMER_LEFT_2_SEQUENCE=TCAGCGATCTCTATTATTCAGATCA PRIMER_RIGHT_2_SEQUENCE=TGACTGGACAGTAAAAAGAAACAT PRIMER_INTERNAL_2_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT PRIMER_LEFT_2=84,25 PRIMER_RIGHT_2=162,24 PRIMER_INTERNAL_2=112,27 PRIMER_LEFT_2_TM=59.411 PRIMER_RIGHT_2_TM=57.077 PRIMER_INTERNAL_2_TM=57.114 PRIMER_LEFT_2_GC_PERCENT=36.000 PRIMER_RIGHT_2_GC_PERCENT=33.333 PRIMER_INTERNAL_2_GC_PERCENT=29.630 PRIMER_LEFT_2_SELF_ANY=9.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=7.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=79 PRIMER_PAIR_3_PENALTY=12.511201 PRIMER_LEFT_3_PENALTY=5.588618 PRIMER_RIGHT_3_PENALTY=6.922583 PRIMER_INTERNAL_3_PENALTY=9.885775 PRIMER_LEFT_3_SEQUENCE=TCAGCGATCTCTATTATTCAGATCA PRIMER_RIGHT_3_SEQUENCE=ATGACTGGACAGTAAAAAGAAACA PRIMER_INTERNAL_3_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT PRIMER_LEFT_3=84,25 PRIMER_RIGHT_3=163,24 PRIMER_INTERNAL_3=112,27 PRIMER_LEFT_3_TM=59.411 PRIMER_RIGHT_3_TM=57.077 PRIMER_INTERNAL_3_TM=57.114 PRIMER_LEFT_3_GC_PERCENT=36.000 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=29.630 PRIMER_LEFT_3_SELF_ANY=9.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=7.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=12.511201 PRIMER_LEFT_4_PENALTY=5.588618 PRIMER_RIGHT_4_PENALTY=6.922583 PRIMER_INTERNAL_4_PENALTY=9.885775 PRIMER_LEFT_4_SEQUENCE=TCATCAGCGATCTCTATTATTCAGA PRIMER_RIGHT_4_SEQUENCE=TGACTGGACAGTAAAAAGAAACAT PRIMER_INTERNAL_4_SEQUENCE=ATGTACCTATTCCTAAGATGTGTTTTT PRIMER_LEFT_4=81,25 PRIMER_RIGHT_4=162,24 PRIMER_INTERNAL_4=112,27 PRIMER_LEFT_4_TM=59.411 PRIMER_RIGHT_4_TM=57.077 PRIMER_INTERNAL_4_TM=57.114 PRIMER_LEFT_4_GC_PERCENT=36.000 PRIMER_RIGHT_4_GC_PERCENT=33.333 PRIMER_INTERNAL_4_GC_PERCENT=29.630 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH523 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGTGTTTCTCGTCAGGGCAAGCCTTATTCACTGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=36,31 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH529 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCCTGATAATTTTGTTTTCTGAAAGTAAATGACAATGTCCTTAATTTTTCGACAAGGGGCTAACTGGATTTGTACCTAGAGGTAGTGAAATNATCTGTGTAGGCATAGTACTGGATTCTAAGGAATNAGTNAACAGGACTTGTGAAAAATCTCCCATTTCTTGCATCTTATATCTTTGATACTTCCATTTATCATGCTGGGGGTAGAGAGTATGAGAAAAGGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCG SEQUENCE_INCLUDED_REGION=36,188 P3_COMMENT=CL is TTCTGAAAGTAAATGACAATGTCC P3_COMMENT=CR is AAGATGCAAGAAATGGGAGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.306229 PRIMER_LEFT_0_PENALTY=1.282673 PRIMER_RIGHT_0_PENALTY=2.023556 PRIMER_INTERNAL_0_PENALTY=6.031815 PRIMER_LEFT_0_SEQUENCE=GGGGCTAACTGGATTTGTACC PRIMER_RIGHT_0_SEQUENCE=GGGAGATTTTTCACAAGTCCTG PRIMER_INTERNAL_0_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA PRIMER_LEFT_0=95,21 PRIMER_RIGHT_0=194,22 PRIMER_INTERNAL_0=141,24 PRIMER_LEFT_0_TM=59.717 PRIMER_RIGHT_0_TM=59.976 PRIMER_INTERNAL_0_TM=57.968 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=4.612671 PRIMER_LEFT_1_PENALTY=2.589115 PRIMER_RIGHT_1_PENALTY=2.023556 PRIMER_INTERNAL_1_PENALTY=6.031815 PRIMER_LEFT_1_SEQUENCE=GGGGCTAACTGGATTTGTACCT PRIMER_RIGHT_1_SEQUENCE=GGGAGATTTTTCACAAGTCCTG PRIMER_INTERNAL_1_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA PRIMER_LEFT_1=95,22 PRIMER_RIGHT_1=194,22 PRIMER_INTERNAL_1=141,24 PRIMER_LEFT_1_TM=60.589 PRIMER_RIGHT_1_TM=59.976 PRIMER_INTERNAL_1_TM=57.968 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_INTERNAL_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=5.019667 PRIMER_LEFT_2_PENALTY=1.282673 PRIMER_RIGHT_2_PENALTY=3.736993 PRIMER_INTERNAL_2_PENALTY=6.031815 PRIMER_LEFT_2_SEQUENCE=GGGGCTAACTGGATTTGTACC PRIMER_RIGHT_2_SEQUENCE=GGGAGATTTTTCACAAGTCCTGT PRIMER_INTERNAL_2_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA PRIMER_LEFT_2=95,21 PRIMER_RIGHT_2=194,23 PRIMER_INTERNAL_2=141,24 PRIMER_LEFT_2_TM=59.717 PRIMER_RIGHT_2_TM=60.737 PRIMER_INTERNAL_2_TM=57.968 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=43.478 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=5.041827 PRIMER_LEFT_3_PENALTY=1.282673 PRIMER_RIGHT_3_PENALTY=3.759153 PRIMER_INTERNAL_3_PENALTY=6.031815 PRIMER_LEFT_3_SEQUENCE=GGGGCTAACTGGATTTGTACC PRIMER_RIGHT_3_SEQUENCE=GGGAGATTTTTCACAAGTCCT PRIMER_INTERNAL_3_SEQUENCE=GGCATAGTACTGGATTCTAAGGAA PRIMER_LEFT_3=95,21 PRIMER_RIGHT_3=194,21 PRIMER_INTERNAL_3=141,24 PRIMER_LEFT_3_TM=59.717 PRIMER_RIGHT_3_TM=57.241 PRIMER_INTERNAL_3_TM=57.968 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=5.288961 PRIMER_LEFT_4_PENALTY=4.006288 PRIMER_RIGHT_4_PENALTY=1.282673 PRIMER_INTERNAL_4_PENALTY=4.336347 PRIMER_LEFT_4_SEQUENCE=TGCCTGATAATTTTGTTTTCTGAA PRIMER_RIGHT_4_SEQUENCE=GGTACAAATCCAGTTAGCCCC PRIMER_INTERNAL_4_SEQUENCE=TGACAATGTCCTTAATTTTTCGA PRIMER_LEFT_4=39,24 PRIMER_RIGHT_4=115,21 PRIMER_INTERNAL_4=69,23 PRIMER_LEFT_4_TM=60.006 PRIMER_RIGHT_4_TM=59.717 PRIMER_INTERNAL_4_TM=58.664 PRIMER_LEFT_4_GC_PERCENT=29.167 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=30.435 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=12.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=77 = SEQUENCE_ID=MH533 SEQUENCE_TEMPLATE=CTTACATTGAAGAGCCAGTAGCGTTTGATAACAAGTTAGAGANNNNAAGTNAATGATGCCTCCAAAAGTTTGATCTGAGAAACACAAAGGATGAAAAGTNAGATGGGAAGAAAGTGGTAGGAACAGACATTTTTNCTTGGTTAGTTAATTGGCTGTTTTGTGTGTGTGTNTNTNTGGTGGGTGGAGAGTNTTGTATGTAGGGGTTGAATAGACCCTCACTTCAGGGCATATTAAGATCAAGATTTCTATGCATCCCATGCTAGATATATACCCAGAAGAAAGGAAGTCTATTGAAAAGATATCTGCACTCCCATGCTTAT SEQUENCE_INCLUDED_REGION=0,320 P3_COMMENT=CL is ACATTGAAGAGCCAGTAGCG P3_COMMENT=CR is GCATGGGATGCATAGAAATC P3_COMMENT=CM is AATGATGCCTCCAAAAGTTTG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.817266 PRIMER_LEFT_0_PENALTY=1.984975 PRIMER_RIGHT_0_PENALTY=1.832292 PRIMER_INTERNAL_0_PENALTY=4.120932 PRIMER_LEFT_0_SEQUENCE=GATTTCTATGCATCCCATGCT PRIMER_RIGHT_0_SEQUENCE=GCATGGGAGTGCAGATATCTT PRIMER_INTERNAL_0_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA PRIMER_LEFT_0=240,21 PRIMER_RIGHT_0=315,21 PRIMER_INTERNAL_0=270,24 PRIMER_LEFT_0_TM=59.015 PRIMER_RIGHT_0_TM=59.168 PRIMER_INTERNAL_0_TM=60.121 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.1000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=10.00 PRIMER_PAIR_0_COMPL_END=8.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=3.874486 PRIMER_LEFT_1_PENALTY=2.042194 PRIMER_RIGHT_1_PENALTY=1.832292 PRIMER_INTERNAL_1_PENALTY=4.120932 PRIMER_LEFT_1_SEQUENCE=AGATTTCTATGCATCCCATGCT PRIMER_RIGHT_1_SEQUENCE=GCATGGGAGTGCAGATATCTT PRIMER_INTERNAL_1_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA PRIMER_LEFT_1=239,22 PRIMER_RIGHT_1=315,21 PRIMER_INTERNAL_1=270,24 PRIMER_LEFT_1_TM=59.958 PRIMER_RIGHT_1_TM=59.168 PRIMER_INTERNAL_1_TM=60.121 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_INTERNAL_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.1000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=10.00 PRIMER_PAIR_1_COMPL_END=8.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=4.193884 PRIMER_LEFT_2_PENALTY=0.072951 PRIMER_RIGHT_2_PENALTY=4.120932 PRIMER_INTERNAL_2_PENALTY=1.984975 PRIMER_LEFT_2_SEQUENCE=GACCCTCACTTCAGGGCATA PRIMER_RIGHT_2_SEQUENCE=TCAATAGACTTCCTTTCTTCTGGG PRIMER_INTERNAL_2_SEQUENCE=GATTTCTATGCATCCCATGCT PRIMER_LEFT_2=210,20 PRIMER_RIGHT_2=293,24 PRIMER_INTERNAL_2=240,21 PRIMER_LEFT_2_TM=60.073 PRIMER_RIGHT_2_TM=60.121 PRIMER_INTERNAL_2_TM=59.015 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=41.667 PRIMER_INTERNAL_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=84 PRIMER_PAIR_3_PENALTY=4.398830 PRIMER_LEFT_3_PENALTY=2.566538 PRIMER_RIGHT_3_PENALTY=1.832292 PRIMER_INTERNAL_3_PENALTY=4.120932 PRIMER_LEFT_3_SEQUENCE=CAAGATTTCTATGCATCCCATG PRIMER_RIGHT_3_SEQUENCE=AAGCATGGGAGTGCAGATATC PRIMER_INTERNAL_3_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA PRIMER_LEFT_3=237,22 PRIMER_RIGHT_3=317,21 PRIMER_INTERNAL_3=270,24 PRIMER_LEFT_3_TM=59.433 PRIMER_RIGHT_3_TM=59.168 PRIMER_INTERNAL_3_TM=60.121 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.4000 PRIMER_RIGHT_3_END_STABILITY=5.5000 PRIMER_PAIR_3_COMPL_ANY=9.00 PRIMER_PAIR_3_COMPL_END=8.00 PRIMER_PAIR_3_PRODUCT_SIZE=81 PRIMER_PAIR_4_PENALTY=4.464586 PRIMER_LEFT_4_PENALTY=1.984975 PRIMER_RIGHT_4_PENALTY=2.479611 PRIMER_INTERNAL_4_PENALTY=4.120932 PRIMER_LEFT_4_SEQUENCE=GATTTCTATGCATCCCATGCT PRIMER_RIGHT_4_SEQUENCE=GCATGGGAGTGCAGATATCTTT PRIMER_INTERNAL_4_SEQUENCE=CCCAGAAGAAAGGAAGTCTATTGA PRIMER_LEFT_4=240,21 PRIMER_RIGHT_4=315,22 PRIMER_INTERNAL_4=270,24 PRIMER_LEFT_4_TM=59.015 PRIMER_RIGHT_4_TM=60.480 PRIMER_INTERNAL_4_TM=60.121 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=45.455 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=10.00 PRIMER_PAIR_4_COMPL_END=6.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH536 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCCTTTTGACAGGGCTTCCAAAAAAAGGCAGNGCGGCCCGGGTGTCTCCCTGGCAGCGGCGAGAAGTCAGCGCCGGGGCTGGGGACTTGTAGGACAGAGTCGNAGNTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTG SEQUENCE_INCLUDED_REGION=36,108 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH539 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTGCATCTCTTTTTCACCTTCTGCATACTGGTGCTGAGCCTAAGGCCTTGAATGTAAGTGCTTTTTAAGTATTTACCAAGTGGAAGTTTCCATTTTATTTTCCACCTTATATTATAGTTGATTGTGTCCTAGTATTCCTCCTAAATTAGTGGCTGAGGAGAGAGAGTGTAGCAGCGATCTACTCCTCTGCTGGGACACACAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC SEQUENCE_INCLUDED_REGION=36,168 P3_COMMENT=CL is TCCTGCATCTCTTTTTCACC P3_COMMENT=CR is CCACTAATTTAGGAGGAATACTAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.082643 PRIMER_LEFT_0_PENALTY=0.168025 PRIMER_RIGHT_0_PENALTY=2.914618 PRIMER_INTERNAL_0_PENALTY=2.328094 PRIMER_LEFT_0_SEQUENCE=TCTGCATACTGGTGCTGAGC PRIMER_RIGHT_0_SEQUENCE=AAAATGGAAACTTCCACTTGG PRIMER_INTERNAL_0_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT PRIMER_LEFT_0=58,20 PRIMER_RIGHT_0=134,21 PRIMER_INTERNAL_0=82,22 PRIMER_LEFT_0_TM=60.168 PRIMER_RIGHT_0_TM=58.085 PRIMER_INTERNAL_0_TM=59.672 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=10.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=3.253950 PRIMER_LEFT_1_PENALTY=0.168025 PRIMER_RIGHT_1_PENALTY=3.085925 PRIMER_INTERNAL_1_PENALTY=2.328094 PRIMER_LEFT_1_SEQUENCE=TCTGCATACTGGTGCTGAGC PRIMER_RIGHT_1_SEQUENCE=AAAATGGAAACTTCCACTTGGT PRIMER_INTERNAL_1_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT PRIMER_LEFT_1=58,20 PRIMER_RIGHT_1=134,22 PRIMER_INTERNAL_1=82,22 PRIMER_LEFT_1_TM=60.168 PRIMER_RIGHT_1_TM=58.914 PRIMER_INTERNAL_1_TM=59.672 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=36.364 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=10.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=3.449505 PRIMER_LEFT_2_PENALTY=0.534888 PRIMER_RIGHT_2_PENALTY=2.914618 PRIMER_INTERNAL_2_PENALTY=2.328094 PRIMER_LEFT_2_SEQUENCE=CCTTCTGCATACTGGTGCTG PRIMER_RIGHT_2_SEQUENCE=AAAATGGAAACTTCCACTTGG PRIMER_INTERNAL_2_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT PRIMER_LEFT_2=55,20 PRIMER_RIGHT_2=134,21 PRIMER_INTERNAL_2=82,22 PRIMER_LEFT_2_TM=59.465 PRIMER_RIGHT_2_TM=58.085 PRIMER_INTERNAL_2_TM=59.672 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=38.095 PRIMER_INTERNAL_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=10.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=3.596239 PRIMER_LEFT_3_PENALTY=0.681621 PRIMER_RIGHT_3_PENALTY=2.914618 PRIMER_INTERNAL_3_PENALTY=0.343950 PRIMER_LEFT_3_SEQUENCE=CACCTTCTGCATACTGGTGC PRIMER_RIGHT_3_SEQUENCE=AAAATGGAAACTTCCACTTGG PRIMER_INTERNAL_3_SEQUENCE=TGAGCCTAAGGCCTTGAATG PRIMER_LEFT_3=53,20 PRIMER_RIGHT_3=134,21 PRIMER_INTERNAL_3=73,20 PRIMER_LEFT_3_TM=59.318 PRIMER_RIGHT_3_TM=58.085 PRIMER_INTERNAL_3_TM=60.344 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=38.095 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=10.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=82 PRIMER_PAIR_4_PENALTY=3.602678 PRIMER_LEFT_4_PENALTY=0.168025 PRIMER_RIGHT_4_PENALTY=3.434653 PRIMER_INTERNAL_4_PENALTY=2.328094 PRIMER_LEFT_4_SEQUENCE=TCTGCATACTGGTGCTGAGC PRIMER_RIGHT_4_SEQUENCE=AAATGGAAACTTCCACTTGGT PRIMER_INTERNAL_4_SEQUENCE=GGCCTTGAATGTAAGTGCTTTT PRIMER_LEFT_4=58,20 PRIMER_RIGHT_4=133,21 PRIMER_INTERNAL_4=82,22 PRIMER_LEFT_4_TM=60.168 PRIMER_RIGHT_4_TM=57.565 PRIMER_INTERNAL_4_TM=59.672 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=10.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH554 SEQUENCE_TEMPLATE=GCTCCACTGGGCCTAGGTATGCCATAGAGGCCACCCCTCTGAAGGGCATAGGTGGTAAATCTTGGTGGCATCCACAGGGTGCCAATGCTGCAGGCATCCAGAGTGTAGGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG SEQUENCE_INCLUDED_REGION=0,110 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.070177 PRIMER_LEFT_0_PENALTY=0.061961 PRIMER_RIGHT_0_PENALTY=0.008216 PRIMER_INTERNAL_0_PENALTY=0.751463 PRIMER_LEFT_0_SEQUENCE=TGGGCCTAGGTATGCCATAG PRIMER_RIGHT_0_SEQUENCE=TACACTCTGGATGCCTGCAG PRIMER_INTERNAL_0_SEQUENCE=GGTAAATCTTGGTGGCATCC PRIMER_LEFT_0=7,20 PRIMER_RIGHT_0=106,20 PRIMER_INTERNAL_0=53,20 PRIMER_LEFT_0_TM=59.938 PRIMER_RIGHT_0_TM=60.008 PRIMER_INTERNAL_0_TM=59.249 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=0.974053 PRIMER_LEFT_1_PENALTY=0.965837 PRIMER_RIGHT_1_PENALTY=0.008216 PRIMER_INTERNAL_1_PENALTY=0.751463 PRIMER_LEFT_1_SEQUENCE=AGGTATGCCATAGAGGCCAC PRIMER_RIGHT_1_SEQUENCE=TACACTCTGGATGCCTGCAG PRIMER_INTERNAL_1_SEQUENCE=GGTAAATCTTGGTGGCATCC PRIMER_LEFT_1=14,20 PRIMER_RIGHT_1=106,20 PRIMER_INTERNAL_1=53,20 PRIMER_LEFT_1_TM=59.034 PRIMER_RIGHT_1_TM=60.008 PRIMER_INTERNAL_1_TM=59.249 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=93 PRIMER_PAIR_2_PENALTY=0.974053 PRIMER_LEFT_2_PENALTY=0.965837 PRIMER_RIGHT_2_PENALTY=0.008216 PRIMER_INTERNAL_2_PENALTY=0.751463 PRIMER_LEFT_2_SEQUENCE=AGGTATGCCATAGAGGCCAC PRIMER_RIGHT_2_SEQUENCE=CTACACTCTGGATGCCTGCA PRIMER_INTERNAL_2_SEQUENCE=GGTAAATCTTGGTGGCATCC PRIMER_LEFT_2=14,20 PRIMER_RIGHT_2=107,20 PRIMER_INTERNAL_2=53,20 PRIMER_LEFT_2_TM=59.034 PRIMER_RIGHT_2_TM=60.008 PRIMER_INTERNAL_2_TM=59.249 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=94 PRIMER_PAIR_3_PENALTY=0.985788 PRIMER_LEFT_3_PENALTY=0.977572 PRIMER_RIGHT_3_PENALTY=0.008216 PRIMER_INTERNAL_3_PENALTY=0.751463 PRIMER_LEFT_3_SEQUENCE=GGGCCTAGGTATGCCATAGA PRIMER_RIGHT_3_SEQUENCE=TACACTCTGGATGCCTGCAG PRIMER_INTERNAL_3_SEQUENCE=GGTAAATCTTGGTGGCATCC PRIMER_LEFT_3=8,20 PRIMER_RIGHT_3=106,20 PRIMER_INTERNAL_3=53,20 PRIMER_LEFT_3_TM=59.022 PRIMER_RIGHT_3_TM=60.008 PRIMER_INTERNAL_3_TM=59.249 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=5.6000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=0.985788 PRIMER_LEFT_4_PENALTY=0.977572 PRIMER_RIGHT_4_PENALTY=0.008216 PRIMER_INTERNAL_4_PENALTY=0.751463 PRIMER_LEFT_4_SEQUENCE=GGGCCTAGGTATGCCATAGA PRIMER_RIGHT_4_SEQUENCE=CTACACTCTGGATGCCTGCA PRIMER_INTERNAL_4_SEQUENCE=GGTAAATCTTGGTGGCATCC PRIMER_LEFT_4=8,20 PRIMER_RIGHT_4=107,20 PRIMER_INTERNAL_4=53,20 PRIMER_LEFT_4_TM=59.022 PRIMER_RIGHT_4_TM=60.008 PRIMER_INTERNAL_4_TM=59.249 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=5.6000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH556 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAATCATTAATAAAATTAAGATGTTAAAACTGAATATCCATCTCACCAATATTTTTTGAGCACCTTCCATATTCCAAGCACTATTCAAGATAGTGGTGGTCAATGAACAGGAAACACAATGTTCTTGTACTTGAGGAGTTTATGTGATGGTTAGAGAGACAGACAATAAACAATAAACAAATAGCACTTCTCATCATGGTGGACCCTAGAGATAAGATATTTCAGGACTACAGCAAGAAGGGGTACCGAGGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGC SEQUENCE_INCLUDED_REGION=36,205 P3_COMMENT=CL is GTTAAAACTGAATATCCATCTCACC P3_COMMENT=CR is CCACCATGATGAGAAGTGCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.420012 PRIMER_LEFT_0_PENALTY=4.355025 PRIMER_RIGHT_0_PENALTY=0.064987 PRIMER_INTERNAL_0_PENALTY=4.129072 PRIMER_LEFT_0_SEQUENCE=CATCTCACCAATATTTTTTGAGCA PRIMER_RIGHT_0_SEQUENCE=TTCCTGTTCATTGACCACCA PRIMER_INTERNAL_0_SEQUENCE=TTCCATATTCCAAGCACTATTCAA PRIMER_LEFT_0=76,24 PRIMER_RIGHT_0=150,20 PRIMER_INTERNAL_0=102,24 PRIMER_LEFT_0_TM=60.355 PRIMER_RIGHT_0_TM=59.935 PRIMER_INTERNAL_0_TM=59.871 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=33.333 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=4.580906 PRIMER_LEFT_1_PENALTY=4.515919 PRIMER_RIGHT_1_PENALTY=0.064987 PRIMER_INTERNAL_1_PENALTY=0.466916 PRIMER_LEFT_1_SEQUENCE=TCCATCTCACCAATATTTTTTGA PRIMER_RIGHT_1_SEQUENCE=TTCCTGTTCATTGACCACCA PRIMER_INTERNAL_1_SEQUENCE=GCACCTTCCATATTCCAAGC PRIMER_LEFT_1=74,23 PRIMER_RIGHT_1=150,20 PRIMER_INTERNAL_1=97,20 PRIMER_LEFT_1_TM=58.484 PRIMER_RIGHT_1_TM=59.935 PRIMER_INTERNAL_1_TM=59.533 PRIMER_LEFT_1_GC_PERCENT=30.435 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=4.670331 PRIMER_LEFT_2_PENALTY=4.605343 PRIMER_RIGHT_2_PENALTY=0.064987 PRIMER_INTERNAL_2_PENALTY=1.427808 PRIMER_LEFT_2_SEQUENCE=TCCATCTCACCAATATTTTTTGAG PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTCATTGACCACCA PRIMER_INTERNAL_2_SEQUENCE=CACCTTCCATATTCCAAGCA PRIMER_LEFT_2=74,24 PRIMER_RIGHT_2=150,20 PRIMER_INTERNAL_2=98,20 PRIMER_LEFT_2_TM=59.395 PRIMER_RIGHT_2_TM=59.935 PRIMER_INTERNAL_2_TM=58.572 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=4.699503 PRIMER_LEFT_3_PENALTY=4.336505 PRIMER_RIGHT_3_PENALTY=0.362999 PRIMER_INTERNAL_3_PENALTY=5.088471 PRIMER_LEFT_3_SEQUENCE=GAACAGGAAACACAATGTTCTTG PRIMER_RIGHT_3_SEQUENCE=TCCACCATGATGAGAAGTGC PRIMER_INTERNAL_3_SEQUENCE=TGTGATGGTTAGAGAGACAGACAA PRIMER_LEFT_3=142,23 PRIMER_RIGHT_3=240,20 PRIMER_INTERNAL_3=180,24 PRIMER_LEFT_3_TM=58.663 PRIMER_RIGHT_3_TM=59.637 PRIMER_INTERNAL_3_TM=58.912 PRIMER_LEFT_3_GC_PERCENT=39.130 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=4.699503 PRIMER_LEFT_4_PENALTY=4.336505 PRIMER_RIGHT_4_PENALTY=0.362999 PRIMER_INTERNAL_4_PENALTY=5.088471 PRIMER_LEFT_4_SEQUENCE=TGAACAGGAAACACAATGTTCTT PRIMER_RIGHT_4_SEQUENCE=TCCACCATGATGAGAAGTGC PRIMER_INTERNAL_4_SEQUENCE=TGTGATGGTTAGAGAGACAGACAA PRIMER_LEFT_4=141,23 PRIMER_RIGHT_4=240,20 PRIMER_INTERNAL_4=180,24 PRIMER_LEFT_4_TM=58.663 PRIMER_RIGHT_4_TM=59.637 PRIMER_INTERNAL_4_TM=58.912 PRIMER_LEFT_4_GC_PERCENT=34.783 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH560 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACGTTCTCCGTCCTATTTTAAGAACTCAGTTCTTCATAAGACTTGTGTGGTTTTCGATTTTTTCCCAAGTCTGGTTGATCCTTGTGTNGTNATTTTTTTAAATGTGTATCGTCTGTTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG SEQUENCE_INCLUDED_REGION=36,121 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.226148 PRIMER_LEFT_0_PENALTY=4.129255 PRIMER_RIGHT_0_PENALTY=0.096893 PRIMER_INTERNAL_0_PENALTY=3.813135 PRIMER_LEFT_0_SEQUENCE=CGTTCTCCGTCCTATTTTAAGAA PRIMER_RIGHT_0_SEQUENCE=GGATCAACCAGACTTGGGAA PRIMER_INTERNAL_0_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA PRIMER_LEFT_0=39,23 PRIMER_RIGHT_0=118,20 PRIMER_INTERNAL_0=72,23 PRIMER_LEFT_0_TM=58.871 PRIMER_RIGHT_0_TM=59.903 PRIMER_INTERNAL_0_TM=59.187 PRIMER_LEFT_0_GC_PERCENT=39.130 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=39.130 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=4.491833 PRIMER_LEFT_1_PENALTY=4.394940 PRIMER_RIGHT_1_PENALTY=0.096893 PRIMER_INTERNAL_1_PENALTY=3.813135 PRIMER_LEFT_1_SEQUENCE=CGTTCTCCGTCCTATTTTAAGA PRIMER_RIGHT_1_SEQUENCE=GGATCAACCAGACTTGGGAA PRIMER_INTERNAL_1_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA PRIMER_LEFT_1=39,22 PRIMER_RIGHT_1=118,20 PRIMER_INTERNAL_1=72,23 PRIMER_LEFT_1_TM=57.605 PRIMER_RIGHT_1_TM=59.903 PRIMER_INTERNAL_1_TM=59.187 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.0000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=4.498797 PRIMER_LEFT_2_PENALTY=4.401904 PRIMER_RIGHT_2_PENALTY=0.096893 PRIMER_INTERNAL_2_PENALTY=3.813135 PRIMER_LEFT_2_SEQUENCE=CGTTCTCCGTCCTATTTTAAGAAC PRIMER_RIGHT_2_SEQUENCE=GGATCAACCAGACTTGGGAA PRIMER_INTERNAL_2_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA PRIMER_LEFT_2=39,24 PRIMER_RIGHT_2=118,20 PRIMER_INTERNAL_2=72,23 PRIMER_LEFT_2_TM=59.598 PRIMER_RIGHT_2_TM=59.903 PRIMER_INTERNAL_2_TM=59.187 PRIMER_LEFT_2_GC_PERCENT=41.667 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=4.498797 PRIMER_LEFT_3_PENALTY=4.401904 PRIMER_RIGHT_3_PENALTY=0.096893 PRIMER_INTERNAL_3_PENALTY=3.813135 PRIMER_LEFT_3_SEQUENCE=ACGTTCTCCGTCCTATTTTAAGAA PRIMER_RIGHT_3_SEQUENCE=GGATCAACCAGACTTGGGAA PRIMER_INTERNAL_3_SEQUENCE=CATAAGACTTGTGTGGTTTTCGA PRIMER_LEFT_3=38,24 PRIMER_RIGHT_3=118,20 PRIMER_INTERNAL_3=72,23 PRIMER_LEFT_3_TM=59.598 PRIMER_RIGHT_3_TM=59.903 PRIMER_INTERNAL_3_TM=59.187 PRIMER_LEFT_3_GC_PERCENT=37.500 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=81 PRIMER_PAIR_4_PENALTY=4.624066 PRIMER_LEFT_4_PENALTY=4.129255 PRIMER_RIGHT_4_PENALTY=0.494811 PRIMER_INTERNAL_4_PENALTY=3.554504 PRIMER_LEFT_4_SEQUENCE=CGTTCTCCGTCCTATTTTAAGAA PRIMER_RIGHT_4_SEQUENCE=AGGATCAACCAGACTTGGGA PRIMER_INTERNAL_4_SEQUENCE=GACTTGTGTGGTTTTCGATTTTT PRIMER_LEFT_4=39,23 PRIMER_RIGHT_4=119,20 PRIMER_INTERNAL_4=77,23 PRIMER_LEFT_4_TM=58.871 PRIMER_RIGHT_4_TM=59.505 PRIMER_INTERNAL_4_TM=59.445 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=34.783 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=81 = SEQUENCE_ID=MH563 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGCGAGAAAGGAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTNAGACGGGCAACAGCTGATTGCCCTTCACCG SEQUENCE_INCLUDED_REGION=36,14 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH568 SEQUENCE_TEMPLATE=TCTGGGGATGCCTAGAACAAAAACAGACACCCTCGGCTTCTTCTCACCAGTCCACATGGGTGCCAAACAATCCCACATTCCTACATCCTCCCCACTGGGCTGCCTCCTTCACAACCTCACCAAACTTGGCTTACCGGGAAGCATAAAGCCAAAGCATTTAGTCTTTTATTGCAACATGGTCTGGCTGCAATACACATTAGATAATGACAGCCGNTGGTTGGAAAATGTCACCTNTNNNTTGNAANTT SEQUENCE_INCLUDED_REGION=0,247 P3_COMMENT=CL is AACAAAAACAGACACCCTCG P3_COMMENT=CR is GTATTGCAGCCAGACCATGT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.085545 PRIMER_LEFT_0_PENALTY=0.005232 PRIMER_RIGHT_0_PENALTY=0.080313 PRIMER_INTERNAL_0_PENALTY=0.902727 PRIMER_LEFT_0_SEQUENCE=ACAAAAACAGACACCCTCGG PRIMER_RIGHT_0_SEQUENCE=CAGTGGGGAGGATGTAGGAA PRIMER_INTERNAL_0_SEQUENCE=CTTCTCACCAGTCCACATGG PRIMER_LEFT_0=16,20 PRIMER_RIGHT_0=96,20 PRIMER_INTERNAL_0=39,20 PRIMER_LEFT_0_TM=60.005 PRIMER_RIGHT_0_TM=59.920 PRIMER_INTERNAL_0_TM=59.097 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=9.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=81 PRIMER_PAIR_1_PENALTY=0.146636 PRIMER_LEFT_1_PENALTY=0.066323 PRIMER_RIGHT_1_PENALTY=0.080313 PRIMER_INTERNAL_1_PENALTY=0.385756 PRIMER_LEFT_1_SEQUENCE=CTGGGGATGCCTAGAACAAA PRIMER_RIGHT_1_SEQUENCE=CAGTGGGGAGGATGTAGGAA PRIMER_INTERNAL_1_SEQUENCE=GGCTTCTTCTCACCAGTCCA PRIMER_LEFT_1=1,20 PRIMER_RIGHT_1=96,20 PRIMER_INTERNAL_1=34,20 PRIMER_LEFT_1_TM=60.066 PRIMER_RIGHT_1_TM=59.920 PRIMER_INTERNAL_1_TM=60.386 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=0.398226 PRIMER_LEFT_2_PENALTY=0.385756 PRIMER_RIGHT_2_PENALTY=0.012470 PRIMER_INTERNAL_2_PENALTY=0.080313 PRIMER_LEFT_2_SEQUENCE=GGCTTCTTCTCACCAGTCCA PRIMER_RIGHT_2_SEQUENCE=GCCAAGTTTGGTGAGGTTGT PRIMER_INTERNAL_2_SEQUENCE=TTCCTACATCCTCCCCACTG PRIMER_LEFT_2=34,20 PRIMER_RIGHT_2=129,20 PRIMER_INTERNAL_2=77,20 PRIMER_LEFT_2_TM=60.386 PRIMER_RIGHT_2_TM=60.012 PRIMER_INTERNAL_2_TM=59.920 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=0.414512 PRIMER_LEFT_3_PENALTY=0.402042 PRIMER_RIGHT_3_PENALTY=0.012470 PRIMER_INTERNAL_3_PENALTY=0.080313 PRIMER_LEFT_3_SEQUENCE=CTCGGCTTCTTCTCACCAGT PRIMER_RIGHT_3_SEQUENCE=GCCAAGTTTGGTGAGGTTGT PRIMER_INTERNAL_3_SEQUENCE=TTCCTACATCCTCCCCACTG PRIMER_LEFT_3=31,20 PRIMER_RIGHT_3=129,20 PRIMER_INTERNAL_3=77,20 PRIMER_LEFT_3_TM=59.598 PRIMER_RIGHT_3_TM=60.012 PRIMER_INTERNAL_3_TM=59.920 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=0.419393 PRIMER_LEFT_4_PENALTY=0.066323 PRIMER_RIGHT_4_PENALTY=0.353069 PRIMER_INTERNAL_4_PENALTY=0.385756 PRIMER_LEFT_4_SEQUENCE=CTGGGGATGCCTAGAACAAA PRIMER_RIGHT_4_SEQUENCE=AGGAATGTGGGATTGTTTGG PRIMER_INTERNAL_4_SEQUENCE=GGCTTCTTCTCACCAGTCCA PRIMER_LEFT_4=1,20 PRIMER_RIGHT_4=81,20 PRIMER_INTERNAL_4=34,20 PRIMER_LEFT_4_TM=60.066 PRIMER_RIGHT_4_TM=59.647 PRIMER_INTERNAL_4_TM=60.386 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=8.8000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=81 = SEQUENCE_ID=MH569 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAGAGATAAGACCTTGAAATAGTAGGATTCTTACAGGTGTATTACATGGTTTGAATTGAGATGATTACATGGGCATGTCTCACCTATAGTCAGGTAAACAAGTCTGGAAATCAAGAAATGGAAATGGTTTCTCTGTTGAACATAATAACCCCTTTGTAGGCTTTTTTAACTTTTTGTCCTGGTAACTATCTTGATTTGGTGCTCTTAGTTTCCAGTAACGGGAATTATTTCATTGGGGTTCCAAGAGGGTTGCATTAAACTTTATGAGACTGCCACTTGACCATTTGAGGATCCTTACGTTACTAAACAAACAGGCAAAAAGGGACTATTCTACTGGCTA SEQUENCE_INCLUDED_REGION=36,342 P3_COMMENT=CL is CATGGGCATGTCTCACCTAT P3_COMMENT=CR is TTTGCCTGTTTGTTTAGTAACG P3_COMMENT=CM is GTCAGGTAAACAAGTCTGGAAATC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.510318 PRIMER_LEFT_0_PENALTY=0.096855 PRIMER_RIGHT_0_PENALTY=0.413463 PRIMER_INTERNAL_0_PENALTY=0.711868 PRIMER_LEFT_0_SEQUENCE=TTTCATTGGGGTTCCAAGAG PRIMER_RIGHT_0_SEQUENCE=TCCCTTTTTGCCTGTTTGTT PRIMER_INTERNAL_0_SEQUENCE=CTGCCACTTGACCATTTGAG PRIMER_LEFT_0=265,20 PRIMER_RIGHT_0=362,20 PRIMER_INTERNAL_0=307,20 PRIMER_LEFT_0_TM=59.903 PRIMER_RIGHT_0_TM=59.587 PRIMER_INTERNAL_0_TM=59.288 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=98 PRIMER_PAIR_1_PENALTY=0.882263 PRIMER_LEFT_1_PENALTY=0.096855 PRIMER_RIGHT_1_PENALTY=0.785407 PRIMER_INTERNAL_1_PENALTY=0.711868 PRIMER_LEFT_1_SEQUENCE=TTTCATTGGGGTTCCAAGAG PRIMER_RIGHT_1_SEQUENCE=AGTCCCTTTTTGCCTGTTTG PRIMER_INTERNAL_1_SEQUENCE=CTGCCACTTGACCATTTGAG PRIMER_LEFT_1=265,20 PRIMER_RIGHT_1=364,20 PRIMER_INTERNAL_1=307,20 PRIMER_LEFT_1_TM=59.903 PRIMER_RIGHT_1_TM=59.215 PRIMER_INTERNAL_1_TM=59.288 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=0.989299 PRIMER_LEFT_2_PENALTY=0.096855 PRIMER_RIGHT_2_PENALTY=0.892444 PRIMER_INTERNAL_2_PENALTY=0.711868 PRIMER_LEFT_2_SEQUENCE=TTTCATTGGGGTTCCAAGAG PRIMER_RIGHT_2_SEQUENCE=CCCTTTTTGCCTGTTTGTTT PRIMER_INTERNAL_2_SEQUENCE=CTGCCACTTGACCATTTGAG PRIMER_LEFT_2=265,20 PRIMER_RIGHT_2=361,20 PRIMER_INTERNAL_2=307,20 PRIMER_LEFT_2_TM=59.903 PRIMER_RIGHT_2_TM=59.108 PRIMER_INTERNAL_2_TM=59.288 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=97 PRIMER_PAIR_3_PENALTY=1.020213 PRIMER_LEFT_3_PENALTY=0.096855 PRIMER_RIGHT_3_PENALTY=0.923358 PRIMER_INTERNAL_3_PENALTY=0.711868 PRIMER_LEFT_3_SEQUENCE=TTTCATTGGGGTTCCAAGAG PRIMER_RIGHT_3_SEQUENCE=GTCCCTTTTTGCCTGTTTGT PRIMER_INTERNAL_3_SEQUENCE=CTGCCACTTGACCATTTGAG PRIMER_LEFT_3=265,20 PRIMER_RIGHT_3=363,20 PRIMER_INTERNAL_3=307,20 PRIMER_LEFT_3_TM=59.903 PRIMER_RIGHT_3_TM=59.077 PRIMER_INTERNAL_3_TM=59.288 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=1.122862 PRIMER_LEFT_4_PENALTY=0.096855 PRIMER_RIGHT_4_PENALTY=1.026007 PRIMER_INTERNAL_4_PENALTY=0.711868 PRIMER_LEFT_4_SEQUENCE=TTTCATTGGGGTTCCAAGAG PRIMER_RIGHT_4_SEQUENCE=AGTCCCTTTTTGCCTGTTTGT PRIMER_INTERNAL_4_SEQUENCE=CTGCCACTTGACCATTTGAG PRIMER_LEFT_4=265,20 PRIMER_RIGHT_4=364,21 PRIMER_INTERNAL_4=307,20 PRIMER_LEFT_4_TM=59.903 PRIMER_RIGHT_4_TM=60.026 PRIMER_INTERNAL_4_TM=59.288 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH570 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGAGATAACGTTGGGAGTNGTAAAAACTCAGTTTATAATCACATTGAATGACATACACTTTAAGGCCCTGAAGTATGTTTCTGTTTTGTCTCTTTTTAAATTCTTATACAGTGGGTATGTGACAGGAATAGAATCAAATGAAGAGGAAATTACAGGGCAGCAACTTTGGACAATAGTGAAAAGGAAAGATGCATTGCTTGCTTTAGTGTTCATGTGACCACTTTATCCTAGAAGCAGAAATTAAGTGGCAGAGAGTCACCTGTGAGTGTGACTAATGCCGTCAATTCCAGGAGAACAAGAAAAAAAAAGCCTTCTGTCCCAACT SEQUENCE_INCLUDED_REGION=36,326 P3_COMMENT=CL is GGCCCTGAAGTATGTTTCTG P3_COMMENT=CR is GTTGGGACAGAAGGCTTTTT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.919073 PRIMER_LEFT_0_PENALTY=0.360621 PRIMER_RIGHT_0_PENALTY=0.558452 PRIMER_INTERNAL_0_PENALTY=4.182061 PRIMER_LEFT_0_SEQUENCE=GGAAAGATGCATTGCTTGCT PRIMER_RIGHT_0_SEQUENCE=CACAGGTGACTCTCTGCCAC PRIMER_INTERNAL_0_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA PRIMER_LEFT_0=220,20 PRIMER_RIGHT_0=301,20 PRIMER_INTERNAL_0=250,24 PRIMER_LEFT_0_TM=60.361 PRIMER_RIGHT_0_TM=59.442 PRIMER_INTERNAL_0_TM=60.182 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=0.919073 PRIMER_LEFT_1_PENALTY=0.360621 PRIMER_RIGHT_1_PENALTY=0.558452 PRIMER_INTERNAL_1_PENALTY=4.182061 PRIMER_LEFT_1_SEQUENCE=AGGAAAGATGCATTGCTTGC PRIMER_RIGHT_1_SEQUENCE=CACAGGTGACTCTCTGCCAC PRIMER_INTERNAL_1_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA PRIMER_LEFT_1=219,20 PRIMER_RIGHT_1=301,20 PRIMER_INTERNAL_1=250,24 PRIMER_LEFT_1_TM=60.361 PRIMER_RIGHT_1_TM=59.442 PRIMER_INTERNAL_1_TM=60.182 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=0.974220 PRIMER_LEFT_2_PENALTY=0.360621 PRIMER_RIGHT_2_PENALTY=0.613599 PRIMER_INTERNAL_2_PENALTY=4.182061 PRIMER_LEFT_2_SEQUENCE=GGAAAGATGCATTGCTTGCT PRIMER_RIGHT_2_SEQUENCE=TCACAGGTGACTCTCTGCCA PRIMER_INTERNAL_2_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA PRIMER_LEFT_2=220,20 PRIMER_RIGHT_2=302,20 PRIMER_INTERNAL_2=250,24 PRIMER_LEFT_2_TM=60.361 PRIMER_RIGHT_2_TM=60.614 PRIMER_INTERNAL_2_TM=60.182 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=0.974220 PRIMER_LEFT_3_PENALTY=0.360621 PRIMER_RIGHT_3_PENALTY=0.613599 PRIMER_INTERNAL_3_PENALTY=4.182061 PRIMER_LEFT_3_SEQUENCE=AGGAAAGATGCATTGCTTGC PRIMER_RIGHT_3_SEQUENCE=TCACAGGTGACTCTCTGCCA PRIMER_INTERNAL_3_SEQUENCE=TGTGACCACTTTATCCTAGAAGCA PRIMER_LEFT_3=219,20 PRIMER_RIGHT_3=302,20 PRIMER_INTERNAL_3=250,24 PRIMER_LEFT_3_TM=60.361 PRIMER_RIGHT_3_TM=60.614 PRIMER_INTERNAL_3_TM=60.182 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=84 PRIMER_PAIR_4_PENALTY=1.653872 PRIMER_LEFT_4_PENALTY=0.558452 PRIMER_RIGHT_4_PENALTY=1.095420 PRIMER_INTERNAL_4_PENALTY=0.460175 PRIMER_LEFT_4_SEQUENCE=GTGGCAGAGAGTCACCTGTG PRIMER_RIGHT_4_SEQUENCE=TGGGACAGAAGGCTTTTTTTT PRIMER_INTERNAL_4_SEQUENCE=CTAATGCCGTCAATTCCAGG PRIMER_LEFT_4=282,20 PRIMER_RIGHT_4=358,21 PRIMER_INTERNAL_4=309,20 PRIMER_LEFT_4_TM=59.442 PRIMER_RIGHT_4_TM=60.095 PRIMER_INTERNAL_4_TM=60.460 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=77 = SEQUENCE_ID=MH572 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAAGGCTGAATCTTAACAGCCTTTTGATGCCAAAGCCACTTTCAGTCTTAATTCTTTTTGGAGCCTAAGATCAGTGCAACCCTCCAAGGCTCCCCAGTATCTGGCACATCTTTCCCTTTTCATCTCCGTTTGTGTGTTTGGCCAAATAATATCTCCCCCAGGGACGTCCTCTTTCTAATCCCTGAAACCTGAGAAAATGTTATCTTATGCAGTGCTATGGTTTGAATGTGTCCCCCACAAAGCACACATTAGAAACTTAATCCCCAGTGCAACAGTNTTGGGAGGTGGGGCCTAATGGAAGATGTTTAGGTCATAAAAGGTACCGAGCTCGAATTCGTAATC SEQUENCE_INCLUDED_REGION=36,283 P3_COMMENT=CL is AATCTTAACAGCCTTTTGATGC P3_COMMENT=CR is TGTTGCACTGGGGATTAAGT P3_COMMENT=CM is AAAGCCACTTTCAGTCTTAATTCT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.298763 PRIMER_LEFT_0_PENALTY=0.248900 PRIMER_RIGHT_0_PENALTY=0.049863 PRIMER_INTERNAL_0_PENALTY=0.300243 PRIMER_LEFT_0_SEQUENCE=CTTTTGATGCCAAAGCCACT PRIMER_RIGHT_0_SEQUENCE=ATGTGCCAGATACTGGGGAG PRIMER_INTERNAL_0_SEQUENCE=GCCTAAGATCAGTGCAACCC PRIMER_LEFT_0=58,20 PRIMER_RIGHT_0=146,20 PRIMER_INTERNAL_0=100,20 PRIMER_LEFT_0_TM=60.249 PRIMER_RIGHT_0_TM=59.950 PRIMER_INTERNAL_0_TM=59.700 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=89 PRIMER_PAIR_1_PENALTY=0.298763 PRIMER_LEFT_1_PENALTY=0.248900 PRIMER_RIGHT_1_PENALTY=0.049863 PRIMER_INTERNAL_1_PENALTY=0.300243 PRIMER_LEFT_1_SEQUENCE=CTTTTGATGCCAAAGCCACT PRIMER_RIGHT_1_SEQUENCE=AGATGTGCCAGATACTGGGG PRIMER_INTERNAL_1_SEQUENCE=GCCTAAGATCAGTGCAACCC PRIMER_LEFT_1=58,20 PRIMER_RIGHT_1=148,20 PRIMER_INTERNAL_1=100,20 PRIMER_LEFT_1_TM=60.249 PRIMER_RIGHT_1_TM=59.950 PRIMER_INTERNAL_1_TM=59.700 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=11.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=0.298763 PRIMER_LEFT_2_PENALTY=0.248900 PRIMER_RIGHT_2_PENALTY=0.049863 PRIMER_INTERNAL_2_PENALTY=0.300243 PRIMER_LEFT_2_SEQUENCE=ACAGCCTTTTGATGCCAAAG PRIMER_RIGHT_2_SEQUENCE=ATGTGCCAGATACTGGGGAG PRIMER_INTERNAL_2_SEQUENCE=GCCTAAGATCAGTGCAACCC PRIMER_LEFT_2=53,20 PRIMER_RIGHT_2=146,20 PRIMER_INTERNAL_2=100,20 PRIMER_LEFT_2_TM=60.249 PRIMER_RIGHT_2_TM=59.950 PRIMER_INTERNAL_2_TM=59.700 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=94 PRIMER_PAIR_3_PENALTY=0.298763 PRIMER_LEFT_3_PENALTY=0.248900 PRIMER_RIGHT_3_PENALTY=0.049863 PRIMER_INTERNAL_3_PENALTY=0.300243 PRIMER_LEFT_3_SEQUENCE=ACAGCCTTTTGATGCCAAAG PRIMER_RIGHT_3_SEQUENCE=AGATGTGCCAGATACTGGGG PRIMER_INTERNAL_3_SEQUENCE=GCCTAAGATCAGTGCAACCC PRIMER_LEFT_3=53,20 PRIMER_RIGHT_3=148,20 PRIMER_INTERNAL_3=100,20 PRIMER_LEFT_3_TM=60.249 PRIMER_RIGHT_3_TM=59.950 PRIMER_INTERNAL_3_TM=59.700 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=11.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=96 PRIMER_PAIR_4_PENALTY=0.309566 PRIMER_LEFT_4_PENALTY=0.259704 PRIMER_RIGHT_4_PENALTY=0.049863 PRIMER_INTERNAL_4_PENALTY=0.300243 PRIMER_LEFT_4_SEQUENCE=ATGCCAAAGCCACTTTCAGT PRIMER_RIGHT_4_SEQUENCE=ATGTGCCAGATACTGGGGAG PRIMER_INTERNAL_4_SEQUENCE=GCCTAAGATCAGTGCAACCC PRIMER_LEFT_4=64,20 PRIMER_RIGHT_4=146,20 PRIMER_INTERNAL_4=100,20 PRIMER_LEFT_4_TM=59.740 PRIMER_RIGHT_4_TM=59.950 PRIMER_INTERNAL_4_TM=59.700 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=83 = SEQUENCE_ID=MH574 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAATTTAACCATTATTAGATTTTCTCCACTCAAGGCATCTCCTCATAAGCAGTNTTTCCTGACCATAAATCTTAGTTGTCCTATCCTCGGACACTTGTTATTTCATCACCCTGCCTTTGCAATTATCTGAAAATATCTTTGGTTATACAATTTATCGTGTCTCCCCACCCAACCCCCATCTCCTAGAACCCACTTCCCTAACCCGAAAATACGGGGCAAGAAACCTGTCTGTACTGGTCACAACCCTATCCCTGGGCCTACAACCGTGTCTGAGTCATAGTAATCCCTCAACAAATTTTGTGTGAACCGAAAGAAAAAAACACTTAAGACAATCTAAG SEQUENCE_INCLUDED_REGION=36,340 P3_COMMENT=CL is TTCTCCACTCAAGGCATCTC P3_COMMENT=CR is ACTCAGACACGGTTGTAGGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.416477 PRIMER_LEFT_0_PENALTY=0.349041 PRIMER_RIGHT_0_PENALTY=0.067436 PRIMER_INTERNAL_0_PENALTY=1.118579 PRIMER_LEFT_0_SEQUENCE=TCCACTCAAGGCATCTCCTC PRIMER_RIGHT_0_SEQUENCE=CAAAGGCAGGGTGATGAAAT PRIMER_INTERNAL_0_SEQUENCE=TGTCCTATCCTCGGACACTTG PRIMER_LEFT_0=62,20 PRIMER_RIGHT_0=156,20 PRIMER_INTERNAL_0=114,21 PRIMER_LEFT_0_TM=60.349 PRIMER_RIGHT_0_TM=59.933 PRIMER_INTERNAL_0_TM=60.119 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=95 PRIMER_PAIR_1_PENALTY=0.416477 PRIMER_LEFT_1_PENALTY=0.349041 PRIMER_RIGHT_1_PENALTY=0.067436 PRIMER_INTERNAL_1_PENALTY=1.118579 PRIMER_LEFT_1_SEQUENCE=TCTCCACTCAAGGCATCTCC PRIMER_RIGHT_1_SEQUENCE=CAAAGGCAGGGTGATGAAAT PRIMER_INTERNAL_1_SEQUENCE=TGTCCTATCCTCGGACACTTG PRIMER_LEFT_1=60,20 PRIMER_RIGHT_1=156,20 PRIMER_INTERNAL_1=114,21 PRIMER_LEFT_1_TM=60.349 PRIMER_RIGHT_1_TM=59.933 PRIMER_INTERNAL_1_TM=60.119 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=97 PRIMER_PAIR_2_PENALTY=0.662644 PRIMER_LEFT_2_PENALTY=0.595208 PRIMER_RIGHT_2_PENALTY=0.067436 PRIMER_INTERNAL_2_PENALTY=1.118579 PRIMER_LEFT_2_SEQUENCE=CTCCACTCAAGGCATCTCCT PRIMER_RIGHT_2_SEQUENCE=CAAAGGCAGGGTGATGAAAT PRIMER_INTERNAL_2_SEQUENCE=TGTCCTATCCTCGGACACTTG PRIMER_LEFT_2=61,20 PRIMER_RIGHT_2=156,20 PRIMER_INTERNAL_2=114,21 PRIMER_LEFT_2_TM=59.405 PRIMER_RIGHT_2_TM=59.933 PRIMER_INTERNAL_2_TM=60.119 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=0.740789 PRIMER_LEFT_3_PENALTY=0.040146 PRIMER_RIGHT_3_PENALTY=0.700643 PRIMER_INTERNAL_3_PENALTY=1.067353 PRIMER_LEFT_3_SEQUENCE=CACCCTGCCTTTGCAATTAT PRIMER_RIGHT_3_SEQUENCE=GGGTTAGGGAAGTGGGTTCT PRIMER_INTERNAL_3_SEQUENCE=AATTTATCGTGTCTCCCCACC PRIMER_LEFT_3=144,20 PRIMER_RIGHT_3=241,20 PRIMER_INTERNAL_3=186,21 PRIMER_LEFT_3_TM=59.960 PRIMER_RIGHT_3_TM=59.299 PRIMER_INTERNAL_3_TM=60.067 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=5.8000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=0.744507 PRIMER_LEFT_4_PENALTY=0.040146 PRIMER_RIGHT_4_PENALTY=0.704360 PRIMER_INTERNAL_4_PENALTY=1.067353 PRIMER_LEFT_4_SEQUENCE=CACCCTGCCTTTGCAATTAT PRIMER_RIGHT_4_SEQUENCE=GTGGGTTCTAGGAGATGGGG PRIMER_INTERNAL_4_SEQUENCE=AATTTATCGTGTCTCCCCACC PRIMER_LEFT_4=144,20 PRIMER_RIGHT_4=230,20 PRIMER_INTERNAL_4=186,21 PRIMER_LEFT_4_TM=59.960 PRIMER_RIGHT_4_TM=60.704 PRIMER_INTERNAL_4_TM=60.067 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_INTERNAL_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=11.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH576 SEQUENCE_TEMPLATE=GCTATATAGACTACAATATGCCTCTTAAATTTATCCCAATTCACTTAAAGTTTATCCTTCCCTGTACTGCAGCCTAAAAACTCCAGGCAGTAATCTGGACAAACGTAGGGCTCAATTCTTTGTTTCTATCTTTTAGGCATCATTGTCCCAGTCTAAATGATGTCTATATTTTCTTTGCATCTTAGTTATTTCAGGTGAGAGATTAAATCATGTCTTTGTTATTCCATCTTGGTTGGAATTGGAAGTCCTAGGTTANGTGTAAATTTAAGAAATGAAAATGATATGCGTGGGCAATTAAGTTGTCAG SEQUENCE_INCLUDED_REGION=0,306 P3_COMMENT=CL is CAATTCACTTAAAGTTTATCCTTCC P3_COMMENT=CR is CTTAATTGCCCACGCATATC P3_COMMENT=CM is TGTACTGCAGCCTAAAAACTCC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.842445 PRIMER_LEFT_0_PENALTY=0.377925 PRIMER_RIGHT_0_PENALTY=0.464520 PRIMER_INTERNAL_0_PENALTY=0.254613 PRIMER_LEFT_0_SEQUENCE=CAGCCTAAAAACTCCAGGCA PRIMER_RIGHT_0_SEQUENCE=AGACTGGGACAATGATGCCT PRIMER_INTERNAL_0_SEQUENCE=TCTGGACAAACGTAGGGCTC PRIMER_LEFT_0=69,20 PRIMER_RIGHT_0=153,20 PRIMER_INTERNAL_0=93,20 PRIMER_LEFT_0_TM=60.378 PRIMER_RIGHT_0_TM=59.535 PRIMER_INTERNAL_0_TM=60.255 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=85 PRIMER_PAIR_1_PENALTY=0.842445 PRIMER_LEFT_1_PENALTY=0.377925 PRIMER_RIGHT_1_PENALTY=0.464520 PRIMER_INTERNAL_1_PENALTY=0.254613 PRIMER_LEFT_1_SEQUENCE=TGCAGCCTAAAAACTCCAGG PRIMER_RIGHT_1_SEQUENCE=AGACTGGGACAATGATGCCT PRIMER_INTERNAL_1_SEQUENCE=TCTGGACAAACGTAGGGCTC PRIMER_LEFT_1=67,20 PRIMER_RIGHT_1=153,20 PRIMER_INTERNAL_1=93,20 PRIMER_LEFT_1_TM=60.378 PRIMER_RIGHT_1_TM=59.535 PRIMER_INTERNAL_1_TM=60.255 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=87 PRIMER_PAIR_2_PENALTY=1.412615 PRIMER_LEFT_2_PENALTY=0.377925 PRIMER_RIGHT_2_PENALTY=1.034689 PRIMER_INTERNAL_2_PENALTY=0.254613 PRIMER_LEFT_2_SEQUENCE=CAGCCTAAAAACTCCAGGCA PRIMER_RIGHT_2_SEQUENCE=TGGGACAATGATGCCTAAAA PRIMER_INTERNAL_2_SEQUENCE=TCTGGACAAACGTAGGGCTC PRIMER_LEFT_2=69,20 PRIMER_RIGHT_2=149,20 PRIMER_INTERNAL_2=93,20 PRIMER_LEFT_2_TM=60.378 PRIMER_RIGHT_2_TM=58.965 PRIMER_INTERNAL_2_TM=60.255 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=1.412615 PRIMER_LEFT_3_PENALTY=0.377925 PRIMER_RIGHT_3_PENALTY=1.034689 PRIMER_INTERNAL_3_PENALTY=0.254613 PRIMER_LEFT_3_SEQUENCE=TGCAGCCTAAAAACTCCAGG PRIMER_RIGHT_3_SEQUENCE=TGGGACAATGATGCCTAAAA PRIMER_INTERNAL_3_SEQUENCE=TCTGGACAAACGTAGGGCTC PRIMER_LEFT_3=67,20 PRIMER_RIGHT_3=149,20 PRIMER_INTERNAL_3=93,20 PRIMER_LEFT_3_TM=60.378 PRIMER_RIGHT_3_TM=58.965 PRIMER_INTERNAL_3_TM=60.255 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=1.430622 PRIMER_LEFT_4_PENALTY=0.377925 PRIMER_RIGHT_4_PENALTY=1.052696 PRIMER_INTERNAL_4_PENALTY=0.254613 PRIMER_LEFT_4_SEQUENCE=CAGCCTAAAAACTCCAGGCA PRIMER_RIGHT_4_SEQUENCE=GACTGGGACAATGATGCCTAA PRIMER_INTERNAL_4_SEQUENCE=TCTGGACAAACGTAGGGCTC PRIMER_LEFT_4=69,20 PRIMER_RIGHT_4=152,21 PRIMER_INTERNAL_4=93,20 PRIMER_LEFT_4_TM=60.378 PRIMER_RIGHT_4_TM=59.947 PRIMER_INTERNAL_4_TM=60.255 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=7.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=84 = SEQUENCE_ID=MH578 SEQUENCE_TEMPLATE=CCNNNNGTTCATGCTCAGGTCGACTCTAGAGGATCCCTGTGGGTTTCATCAGACCTGAGATTCAGCATGAAATCTACCAAAGGTACCACAAATGTAACCTTGTCCAAAACGAATCTCAGTTTCTGCATATGTAAAATGGGAATGATAAGAGCACCCACCTACCTCATGCAACTGTTGAGAGAAATAAATGAGACATTGTAAGTAAAGTTTGTAATGCACTGTTATGGCCTGAATTGTGTACCCTAAAATTCATATGTTGAAGCCCTAACACCCAATATGNCTGTATTTGTACATAAGGCCTTTAGGGNGGTAATTAAGGTTAAATG SEQUENCE_INCLUDED_REGION=37,289 P3_COMMENT=CL is TCATCAGACCTGAGATTCAGC P3_COMMENT=CR is TAAAGGCCTTATGTACAAATACAG P3_COMMENT=CM is TGAAATCTACCAAAGGTACCACA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.334266 PRIMER_LEFT_0_PENALTY=0.010545 PRIMER_RIGHT_0_PENALTY=0.323721 PRIMER_INTERNAL_0_PENALTY=8.866785 PRIMER_LEFT_0_SEQUENCE=CCCACCTACCTCATGCAACT PRIMER_RIGHT_0_SEQUENCE=GGGTACACAATTCAGGCCAT PRIMER_INTERNAL_0_SEQUENCE=GTTGAGAGAAATAAATGAGACATTGT PRIMER_LEFT_0=153,20 PRIMER_RIGHT_0=242,20 PRIMER_INTERNAL_0=173,26 PRIMER_LEFT_0_TM=59.989 PRIMER_RIGHT_0_TM=59.676 PRIMER_INTERNAL_0_TM=57.133 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=30.769 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=9.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=90 PRIMER_PAIR_1_PENALTY=0.385635 PRIMER_LEFT_1_PENALTY=0.010545 PRIMER_RIGHT_1_PENALTY=0.375090 PRIMER_INTERNAL_1_PENALTY=8.866785 PRIMER_LEFT_1_SEQUENCE=CCCACCTACCTCATGCAACT PRIMER_RIGHT_1_SEQUENCE=AGGGTACACAATTCAGGCCA PRIMER_INTERNAL_1_SEQUENCE=GTTGAGAGAAATAAATGAGACATTGT PRIMER_LEFT_1=153,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=173,26 PRIMER_LEFT_1_TM=59.989 PRIMER_RIGHT_1_TM=60.375 PRIMER_INTERNAL_1_TM=57.133 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=30.769 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=11.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=0.473115 PRIMER_LEFT_2_PENALTY=0.149395 PRIMER_RIGHT_2_PENALTY=0.323721 PRIMER_INTERNAL_2_PENALTY=7.198789 PRIMER_LEFT_2_SEQUENCE=ACCCACCTACCTCATGCAAC PRIMER_RIGHT_2_SEQUENCE=GGGTACACAATTCAGGCCAT PRIMER_INTERNAL_2_SEQUENCE=TGTTGAGAGAAATAAATGAGACATTG PRIMER_LEFT_2=152,20 PRIMER_RIGHT_2=242,20 PRIMER_INTERNAL_2=172,26 PRIMER_LEFT_2_TM=59.851 PRIMER_RIGHT_2_TM=59.676 PRIMER_INTERNAL_2_TM=58.801 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=30.769 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=91 PRIMER_PAIR_3_PENALTY=0.524485 PRIMER_LEFT_3_PENALTY=0.149395 PRIMER_RIGHT_3_PENALTY=0.375090 PRIMER_INTERNAL_3_PENALTY=7.198789 PRIMER_LEFT_3_SEQUENCE=ACCCACCTACCTCATGCAAC PRIMER_RIGHT_3_SEQUENCE=AGGGTACACAATTCAGGCCA PRIMER_INTERNAL_3_SEQUENCE=TGTTGAGAGAAATAAATGAGACATTG PRIMER_LEFT_3=152,20 PRIMER_RIGHT_3=243,20 PRIMER_INTERNAL_3=172,26 PRIMER_LEFT_3_TM=59.851 PRIMER_RIGHT_3_TM=60.375 PRIMER_INTERNAL_3_TM=58.801 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=30.769 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=11.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=92 PRIMER_PAIR_4_PENALTY=0.602568 PRIMER_LEFT_4_PENALTY=0.278847 PRIMER_RIGHT_4_PENALTY=0.323721 PRIMER_INTERNAL_4_PENALTY=5.173020 PRIMER_LEFT_4_SEQUENCE=AAGAGCACCCACCTACCTCA PRIMER_RIGHT_4_SEQUENCE=GGGTACACAATTCAGGCCAT PRIMER_INTERNAL_4_SEQUENCE=TGCAACTGTTGAGAGAAATAAATGA PRIMER_LEFT_4=146,20 PRIMER_RIGHT_4=242,20 PRIMER_INTERNAL_4=166,25 PRIMER_LEFT_4_TM=59.721 PRIMER_RIGHT_4_TM=59.676 PRIMER_INTERNAL_4_TM=60.173 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=32.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=9.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=97 = SEQUENCE_ID=MH584 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGAATTGACCCACAGCATCCCCACAGAATGTGATTTCTTGAGTCCTTTGTGAAGGTCACCTCCTCTAACCTCTCTATATATACATACTCAAACTTCTTTGGAGACTGGAAGATTGGAGGTACTATCTTTTAGAGTAGTTTTGCTGAAAAGTGATAAACTCCTTTCACAGTGTGGGAAAAGAGATCCAGTAAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCT SEQUENCE_INCLUDED_REGION=34,161 P3_COMMENT=CL is TCTGAATTGACCCACAGCAT P3_COMMENT=CR is ACTTTTCAGCAAAACTACTCTAAAA P3_COMMENT=CM is CCCACAGAATGTGATTTCTTG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.948659 PRIMER_LEFT_0_PENALTY=1.604487 PRIMER_RIGHT_0_PENALTY=1.344173 PRIMER_INTERNAL_0_PENALTY=0.450843 PRIMER_LEFT_0_SEQUENCE=TCCCCACAGAATGTGATTTCT PRIMER_RIGHT_0_SEQUENCE=TCCAATCTTCCAGTCTCCAAA PRIMER_INTERNAL_0_SEQUENCE=CCTTTGTGAAGGTCACCTCC PRIMER_LEFT_0=54,21 PRIMER_RIGHT_0=153,21 PRIMER_INTERNAL_0=80,20 PRIMER_LEFT_0_TM=59.396 PRIMER_RIGHT_0_TM=59.656 PRIMER_INTERNAL_0_TM=59.549 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=7.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=3.013129 PRIMER_LEFT_1_PENALTY=1.668956 PRIMER_RIGHT_1_PENALTY=1.344173 PRIMER_INTERNAL_1_PENALTY=0.450843 PRIMER_LEFT_1_SEQUENCE=TCCCCACAGAATGTGATTTC PRIMER_RIGHT_1_SEQUENCE=TCCAATCTTCCAGTCTCCAAA PRIMER_INTERNAL_1_SEQUENCE=CCTTTGTGAAGGTCACCTCC PRIMER_LEFT_1=54,20 PRIMER_RIGHT_1=153,21 PRIMER_INTERNAL_1=80,20 PRIMER_LEFT_1_TM=58.331 PRIMER_RIGHT_1_TM=59.656 PRIMER_INTERNAL_1_TM=59.549 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=7.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=8.8000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=3.365079 PRIMER_LEFT_2_PENALTY=2.029253 PRIMER_RIGHT_2_PENALTY=1.335825 PRIMER_INTERNAL_2_PENALTY=0.450843 PRIMER_LEFT_2_SEQUENCE=CCCACAGAATGTGATTTCTTGA PRIMER_RIGHT_2_SEQUENCE=CCTCCAATCTTCCAGTCTCC PRIMER_INTERNAL_2_SEQUENCE=CCTTTGTGAAGGTCACCTCC PRIMER_LEFT_2=56,22 PRIMER_RIGHT_2=155,20 PRIMER_INTERNAL_2=80,20 PRIMER_LEFT_2_TM=59.971 PRIMER_RIGHT_2_TM=58.664 PRIMER_INTERNAL_2_TM=59.549 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=7.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=3.373426 PRIMER_LEFT_3_PENALTY=2.029253 PRIMER_RIGHT_3_PENALTY=1.344173 PRIMER_INTERNAL_3_PENALTY=0.450843 PRIMER_LEFT_3_SEQUENCE=CCCACAGAATGTGATTTCTTGA PRIMER_RIGHT_3_SEQUENCE=TCCAATCTTCCAGTCTCCAAA PRIMER_INTERNAL_3_SEQUENCE=CCTTTGTGAAGGTCACCTCC PRIMER_LEFT_3=56,22 PRIMER_RIGHT_3=153,21 PRIMER_INTERNAL_3=80,20 PRIMER_LEFT_3_TM=59.971 PRIMER_RIGHT_3_TM=59.656 PRIMER_INTERNAL_3_TM=59.549 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=7.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=8.8000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=3.403359 PRIMER_LEFT_4_PENALTY=1.604487 PRIMER_RIGHT_4_PENALTY=1.798872 PRIMER_INTERNAL_4_PENALTY=0.450843 PRIMER_LEFT_4_SEQUENCE=TCCCCACAGAATGTGATTTCT PRIMER_RIGHT_4_SEQUENCE=TCCAATCTTCCAGTCTCCAA PRIMER_INTERNAL_4_SEQUENCE=CCTTTGTGAAGGTCACCTCC PRIMER_LEFT_4=54,21 PRIMER_RIGHT_4=153,20 PRIMER_INTERNAL_4=80,20 PRIMER_LEFT_4_TM=59.396 PRIMER_RIGHT_4_TM=58.201 PRIMER_INTERNAL_4_TM=59.549 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=7.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH585 SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCTGCACCAGATCTTTACCCTCTGGGGGGCTGAATCAACGAGTNCTAGACTGAAGCACCCAGGCACAGCCAAGGGTGAGGGAGCCATGATCCTGAACATAGCATTGACTGATTAATGAGACCCCCTGGGCCTGTCTTCAGTGCACTCCCAGTATGGGGACAGCCAGACTGGCGCACCCCCTCCGTGAGGCAGGAGACCCTAGGAGCGCTCTCTGGGGACAGTGACTGCAACTCTCGGTTCCCTTACAAATGGCTGAGTTGTCACCAGAAAATGACACTCACATATGGCAAACCCTGTCCT SEQUENCE_INCLUDED_REGION=34,298 P3_COMMENT=CL is TGCACCAGATCTTTACCCTC P3_COMMENT=CR is CTCAGCCATTTGTAAGGGAA P3_COMMENT=CM is CTAGACTGAAGCACCCAGGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.268600 PRIMER_LEFT_0_PENALTY=0.012470 PRIMER_RIGHT_0_PENALTY=0.256130 PRIMER_INTERNAL_0_PENALTY=0.106742 PRIMER_LEFT_0_SEQUENCE=CTAGACTGAAGCACCCAGGC PRIMER_RIGHT_0_SEQUENCE=CCAGGGGGTCTCATTAATCA PRIMER_INTERNAL_0_SEQUENCE=GGAGCCATGATCCTGAACAT PRIMER_LEFT_0=76,20 PRIMER_RIGHT_0=159,20 PRIMER_INTERNAL_0=111,20 PRIMER_LEFT_0_TM=60.012 PRIMER_RIGHT_0_TM=59.744 PRIMER_INTERNAL_0_TM=59.893 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=84 PRIMER_PAIR_1_PENALTY=0.276782 PRIMER_LEFT_1_PENALTY=0.022009 PRIMER_RIGHT_1_PENALTY=0.254773 PRIMER_INTERNAL_1_PENALTY=1.359444 PRIMER_LEFT_1_SEQUENCE=TGTCTTCAGTGCACTCCCAG PRIMER_RIGHT_1_SEQUENCE=AGAGAGCGCTCCTAGGGTCT PRIMER_INTERNAL_1_SEQUENCE=TATGGGGACAGCCAGACTG PRIMER_LEFT_1=163,20 PRIMER_RIGHT_1=244,20 PRIMER_INTERNAL_1=183,19 PRIMER_LEFT_1_TM=60.022 PRIMER_RIGHT_1_TM=59.745 PRIMER_INTERNAL_1_TM=59.641 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=0.276782 PRIMER_LEFT_2_PENALTY=0.022009 PRIMER_RIGHT_2_PENALTY=0.254773 PRIMER_INTERNAL_2_PENALTY=0.532607 PRIMER_LEFT_2_SEQUENCE=CTGTCTTCAGTGCACTCCCA PRIMER_RIGHT_2_SEQUENCE=AGAGAGCGCTCCTAGGGTCT PRIMER_INTERNAL_2_SEQUENCE=GTATGGGGACAGCCAGACTG PRIMER_LEFT_2=162,20 PRIMER_RIGHT_2=244,20 PRIMER_INTERNAL_2=182,20 PRIMER_LEFT_2_TM=60.022 PRIMER_RIGHT_2_TM=59.745 PRIMER_INTERNAL_2_TM=60.533 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=0.371384 PRIMER_LEFT_3_PENALTY=0.022009 PRIMER_RIGHT_3_PENALTY=0.349375 PRIMER_INTERNAL_3_PENALTY=0.532607 PRIMER_LEFT_3_SEQUENCE=CTGTCTTCAGTGCACTCCCA PRIMER_RIGHT_3_SEQUENCE=CTCCTAGGGTCTCCTGCCTC PRIMER_INTERNAL_3_SEQUENCE=GTATGGGGACAGCCAGACTG PRIMER_LEFT_3=162,20 PRIMER_RIGHT_3=236,20 PRIMER_INTERNAL_3=182,20 PRIMER_LEFT_3_TM=60.022 PRIMER_RIGHT_3_TM=60.349 PRIMER_INTERNAL_3_TM=60.533 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=0.405684 PRIMER_LEFT_4_PENALTY=0.106742 PRIMER_RIGHT_4_PENALTY=0.298942 PRIMER_INTERNAL_4_PENALTY=0.256130 PRIMER_LEFT_4_SEQUENCE=GGAGCCATGATCCTGAACAT PRIMER_RIGHT_4_SEQUENCE=CCATACTGGGAGTGCACTGA PRIMER_INTERNAL_4_SEQUENCE=TGATTAATGAGACCCCCTGG PRIMER_LEFT_4=111,20 PRIMER_RIGHT_4=187,20 PRIMER_INTERNAL_4=140,20 PRIMER_LEFT_4_TM=59.893 PRIMER_RIGHT_4_TM=59.701 PRIMER_INTERNAL_4_TM=59.744 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=77 = SEQUENCE_ID=MH590 SEQUENCE_TEMPLATE=GTTCATGCTGAGGTCGACTCTAGAGGATCCCCCTACCTTCATATACTATACTGTCTCTGTTGAAGGAGGAAATAGTGCAAAATAAACTACTTTGATATGCTGGATCTTGTTAATGAGTNATTCATCTAGTNAGCAAATCATTACTGTTCACCTTATGTGTGCCAGATATTACATCAACACTAGGGATCCTGTAGGTNNGGAGGGAAGTCCCTGCCTTTGTGAAACTTCAAATCAAATGTGGGAGACAGACACTAATCCAAAGAATTTCACCAGTAAG SEQUENCE_INCLUDED_REGION=32,245 P3_COMMENT=CL is ACTATACTGTCTCTGTTGAAGGAGG P3_COMMENT=CR is TGTCTGTCTCCCACATTTGA P3_COMMENT=CM is AACTACTTTGATATGCTGGATCTTG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.607801 PRIMER_LEFT_0_PENALTY=1.375502 PRIMER_RIGHT_0_PENALTY=0.232299 PRIMER_INTERNAL_0_PENALTY=3.799512 PRIMER_LEFT_0_SEQUENCE=TGTTCACCTTATGTGTGCCAG PRIMER_RIGHT_0_SEQUENCE=TTCACAAAGGCAGGGACTTC PRIMER_INTERNAL_0_SEQUENCE=TCAACACTAGGGATCCTGTAGG PRIMER_LEFT_0=144,21 PRIMER_RIGHT_0=222,20 PRIMER_INTERNAL_0=173,22 PRIMER_LEFT_0_TM=59.624 PRIMER_RIGHT_0_TM=60.232 PRIMER_INTERNAL_0_TM=58.200 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=79 PRIMER_PAIR_1_PENALTY=1.607801 PRIMER_LEFT_1_PENALTY=1.375502 PRIMER_RIGHT_1_PENALTY=0.232299 PRIMER_INTERNAL_1_PENALTY=3.799512 PRIMER_LEFT_1_SEQUENCE=CTGTTCACCTTATGTGTGCCA PRIMER_RIGHT_1_SEQUENCE=TTCACAAAGGCAGGGACTTC PRIMER_INTERNAL_1_SEQUENCE=TCAACACTAGGGATCCTGTAGG PRIMER_LEFT_1=143,21 PRIMER_RIGHT_1=222,20 PRIMER_INTERNAL_1=173,22 PRIMER_LEFT_1_TM=59.624 PRIMER_RIGHT_1_TM=60.232 PRIMER_INTERNAL_1_TM=58.200 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=10.0000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=1.664306 PRIMER_LEFT_2_PENALTY=1.375502 PRIMER_RIGHT_2_PENALTY=0.288804 PRIMER_INTERNAL_2_PENALTY=3.799512 PRIMER_LEFT_2_SEQUENCE=TGTTCACCTTATGTGTGCCAG PRIMER_RIGHT_2_SEQUENCE=TTTCACAAAGGCAGGGACTT PRIMER_INTERNAL_2_SEQUENCE=TCAACACTAGGGATCCTGTAGG PRIMER_LEFT_2=144,21 PRIMER_RIGHT_2=223,20 PRIMER_INTERNAL_2=173,22 PRIMER_LEFT_2_TM=59.624 PRIMER_RIGHT_2_TM=59.711 PRIMER_INTERNAL_2_TM=58.200 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=1.664306 PRIMER_LEFT_3_PENALTY=1.375502 PRIMER_RIGHT_3_PENALTY=0.288804 PRIMER_INTERNAL_3_PENALTY=3.799512 PRIMER_LEFT_3_SEQUENCE=TGTTCACCTTATGTGTGCCAG PRIMER_RIGHT_3_SEQUENCE=AAGTTTCACAAAGGCAGGGA PRIMER_INTERNAL_3_SEQUENCE=TCAACACTAGGGATCCTGTAGG PRIMER_LEFT_3=144,21 PRIMER_RIGHT_3=226,20 PRIMER_INTERNAL_3=173,22 PRIMER_LEFT_3_TM=59.624 PRIMER_RIGHT_3_TM=59.711 PRIMER_INTERNAL_3_TM=58.200 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=1.664306 PRIMER_LEFT_4_PENALTY=1.375502 PRIMER_RIGHT_4_PENALTY=0.288804 PRIMER_INTERNAL_4_PENALTY=3.799512 PRIMER_LEFT_4_SEQUENCE=TGTTCACCTTATGTGTGCCAG PRIMER_RIGHT_4_SEQUENCE=GAAGTTTCACAAAGGCAGGG PRIMER_INTERNAL_4_SEQUENCE=TCAACACTAGGGATCCTGTAGG PRIMER_LEFT_4=144,21 PRIMER_RIGHT_4=227,20 PRIMER_INTERNAL_4=173,22 PRIMER_LEFT_4_TM=59.624 PRIMER_RIGHT_4_TM=59.711 PRIMER_INTERNAL_4_TM=58.200 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=84 = SEQUENCE_ID=MH592 SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGCAGTNNCCCCCACAGCAGGGTNCCACCTTTCAGAGTNNAAACAGGTCCTCACTGAACAGTCAATGTCACTTCCAGTCGGGGCACACTGTGTCCNAAGTTTACAATTNNAAACTCTGCTGTCTTGAGAAGNACTGTGGTTAATTCCTGGTACAAACCTTGCATCAGGAGAGTCCTATGCCATCTTCCACTAAAGCAATCAAGTGTGAACANCAGTNCAGAGGGGCTGGTCTGGCCTTAACAATCCTCAGATGGTGCCAGAGTCGGTAGATGCCAGGGGTAC SEQUENCE_INCLUDED_REGION=33,221 P3_COMMENT=CL is AGTCAATGTCACTTCCAGTCG P3_COMMENT=CR is GTTCACACTTGATTGCTTTAGTG P3_COMMENT=CM is CATTGACTGTTCAGTGAGGACC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.907416 PRIMER_LEFT_0_PENALTY=2.165390 PRIMER_RIGHT_0_PENALTY=0.742026 PRIMER_INTERNAL_0_PENALTY=5.383533 PRIMER_LEFT_0_SEQUENCE=AGTCGGGGCACACTGTGT PRIMER_RIGHT_0_SEQUENCE=GGACTCTCCTGATGCAAGGT PRIMER_INTERNAL_0_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA PRIMER_LEFT_0=111,18 PRIMER_RIGHT_0=210,20 PRIMER_INTERNAL_0=168,23 PRIMER_LEFT_0_TM=60.165 PRIMER_RIGHT_0_TM=59.258 PRIMER_INTERNAL_0_TM=57.616 PRIMER_LEFT_0_GC_PERCENT=61.111 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=39.130 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=7.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=3.825155 PRIMER_LEFT_1_PENALTY=3.229947 PRIMER_RIGHT_1_PENALTY=0.595208 PRIMER_INTERNAL_1_PENALTY=4.885646 PRIMER_LEFT_1_SEQUENCE=GTCGGGGCACACTGTGTC PRIMER_RIGHT_1_SEQUENCE=AGGACTCTCCTGATGCAAGG PRIMER_INTERNAL_1_SEQUENCE=CTGTGGTTAATTCCTGGTACAAA PRIMER_LEFT_1=112,18 PRIMER_RIGHT_1=211,20 PRIMER_INTERNAL_1=169,23 PRIMER_LEFT_1_TM=61.230 PRIMER_RIGHT_1_TM=59.405 PRIMER_INTERNAL_1_TM=58.114 PRIMER_LEFT_1_GC_PERCENT=66.667 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=7.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.1000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=3.971973 PRIMER_LEFT_2_PENALTY=3.229947 PRIMER_RIGHT_2_PENALTY=0.742026 PRIMER_INTERNAL_2_PENALTY=5.383533 PRIMER_LEFT_2_SEQUENCE=GTCGGGGCACACTGTGTC PRIMER_RIGHT_2_SEQUENCE=GGACTCTCCTGATGCAAGGT PRIMER_INTERNAL_2_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA PRIMER_LEFT_2=112,18 PRIMER_RIGHT_2=210,20 PRIMER_INTERNAL_2=168,23 PRIMER_LEFT_2_TM=61.230 PRIMER_RIGHT_2_TM=59.258 PRIMER_INTERNAL_2_TM=57.616 PRIMER_LEFT_2_GC_PERCENT=66.667 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=7.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.1000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=3.991869 PRIMER_LEFT_3_PENALTY=3.249843 PRIMER_RIGHT_3_PENALTY=0.742026 PRIMER_INTERNAL_3_PENALTY=5.383533 PRIMER_LEFT_3_SEQUENCE=AGTCGGGGCACACTGTGTC PRIMER_RIGHT_3_SEQUENCE=GGACTCTCCTGATGCAAGGT PRIMER_INTERNAL_3_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA PRIMER_LEFT_3=111,19 PRIMER_RIGHT_3=210,20 PRIMER_INTERNAL_3=168,23 PRIMER_LEFT_3_TM=62.250 PRIMER_RIGHT_3_TM=59.258 PRIMER_INTERNAL_3_TM=57.616 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=7.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.1000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=4.497249 PRIMER_LEFT_4_PENALTY=3.229947 PRIMER_RIGHT_4_PENALTY=1.267302 PRIMER_INTERNAL_4_PENALTY=5.383533 PRIMER_LEFT_4_SEQUENCE=GTCGGGGCACACTGTGTC PRIMER_RIGHT_4_SEQUENCE=AGGACTCTCCTGATGCAAGGT PRIMER_INTERNAL_4_SEQUENCE=ACTGTGGTTAATTCCTGGTACAA PRIMER_LEFT_4=112,18 PRIMER_RIGHT_4=211,21 PRIMER_INTERNAL_4=168,23 PRIMER_LEFT_4_TM=61.230 PRIMER_RIGHT_4_TM=60.267 PRIMER_INTERNAL_4_TM=57.616 PRIMER_LEFT_4_GC_PERCENT=66.667 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=7.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.1000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH599 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTAGAGGNNNCTTATTTTTNCTTTTGTTAAAAAATCAATTTATTACTTTTAAAGTAGTTTTTGTAGATAAAGTTGCTAGAATATGTTGCTAAGCAGGTATGCTTTAAGANCTAGATAAGAAAAAAAATTTCTCATTATTAGGCAACAATTTGTATTGGTCGATTTGCAGCAACCAGAGAAGGAANCCAAACCACAATCCCTGCAGCAACCAGTCTGGGAAGTCAANCCACAATCTCTGCAATCATCAGCCCAGAATAATCAGGAAATGGCCAATGGCTGCCATATTTGTATTTGTTATGTAATTGTTGG SEQUENCE_INCLUDED_REGION=36,312 P3_COMMENT=CL is AGTTGCTAGAATATGTTGCTAAGC P3_COMMENT=CR is GGCTGATGATTGCAGAGATT P3_COMMENT=CM is AAAAATTTCTCATTATTAGGCAACA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.404000 PRIMER_LEFT_0_PENALTY=0.215254 PRIMER_RIGHT_0_PENALTY=0.188746 PRIMER_INTERNAL_0_PENALTY=0.298018 PRIMER_LEFT_0_SEQUENCE=TATTGGTCGATTTGCAGCAA PRIMER_RIGHT_0_SEQUENCE=GGGCTGATGATTGCAGAGAT PRIMER_INTERNAL_0_SEQUENCE=CAGCAACCAGTCTGGGAAGT PRIMER_LEFT_0=191,20 PRIMER_RIGHT_0=289,20 PRIMER_INTERNAL_0=241,20 PRIMER_LEFT_0_TM=60.215 PRIMER_RIGHT_0_TM=60.189 PRIMER_INTERNAL_0_TM=60.298 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=7.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=6.3000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=99 PRIMER_PAIR_1_PENALTY=0.494699 PRIMER_LEFT_1_PENALTY=0.305953 PRIMER_RIGHT_1_PENALTY=0.188746 PRIMER_INTERNAL_1_PENALTY=0.298018 PRIMER_LEFT_1_SEQUENCE=GTATTGGTCGATTTGCAGCA PRIMER_RIGHT_1_SEQUENCE=GGGCTGATGATTGCAGAGAT PRIMER_INTERNAL_1_SEQUENCE=CAGCAACCAGTCTGGGAAGT PRIMER_LEFT_1=190,20 PRIMER_RIGHT_1=289,20 PRIMER_INTERNAL_1=241,20 PRIMER_LEFT_1_TM=59.694 PRIMER_RIGHT_1_TM=60.189 PRIMER_INTERNAL_1_TM=60.298 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=6.3000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=1.019458 PRIMER_LEFT_2_PENALTY=0.298018 PRIMER_RIGHT_2_PENALTY=0.721440 PRIMER_INTERNAL_2_PENALTY=0.188746 PRIMER_LEFT_2_SEQUENCE=CAGCAACCAGTCTGGGAAGT PRIMER_RIGHT_2_SEQUENCE=ACAAATATGGCAGCCATTGG PRIMER_INTERNAL_2_SEQUENCE=ATCTCTGCAATCATCAGCCC PRIMER_LEFT_2=241,20 PRIMER_RIGHT_2=327,20 PRIMER_INTERNAL_2=270,20 PRIMER_LEFT_2_TM=60.298 PRIMER_RIGHT_2_TM=60.721 PRIMER_INTERNAL_2_TM=60.189 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=9.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=8.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=87 PRIMER_PAIR_3_PENALTY=1.186911 PRIMER_LEFT_3_PENALTY=0.998165 PRIMER_RIGHT_3_PENALTY=0.188746 PRIMER_INTERNAL_3_PENALTY=0.298018 PRIMER_LEFT_3_SEQUENCE=GATTTGCAGCAACCAGAGAA PRIMER_RIGHT_3_SEQUENCE=GGGCTGATGATTGCAGAGAT PRIMER_INTERNAL_3_SEQUENCE=CAGCAACCAGTCTGGGAAGT PRIMER_LEFT_3=199,20 PRIMER_RIGHT_3=289,20 PRIMER_INTERNAL_3=241,20 PRIMER_LEFT_3_TM=59.002 PRIMER_RIGHT_3_TM=60.189 PRIMER_INTERNAL_3_TM=60.298 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.3000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=91 PRIMER_PAIR_4_PENALTY=1.196415 PRIMER_LEFT_4_PENALTY=1.007669 PRIMER_RIGHT_4_PENALTY=0.188746 PRIMER_INTERNAL_4_PENALTY=0.298018 PRIMER_LEFT_4_SEQUENCE=GATTTGCAGCAACCAGAGAAG PRIMER_RIGHT_4_SEQUENCE=GGGCTGATGATTGCAGAGAT PRIMER_INTERNAL_4_SEQUENCE=CAGCAACCAGTCTGGGAAGT PRIMER_LEFT_4=199,21 PRIMER_RIGHT_4=289,20 PRIMER_INTERNAL_4=241,20 PRIMER_LEFT_4_TM=60.008 PRIMER_RIGHT_4_TM=60.189 PRIMER_INTERNAL_4_TM=60.298 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.3000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH604 SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTGTNNATCGCTTCCTCAGAGANNCCGTCCCTGACTCCCAGACAAGGNCGGGCTGGGCCTTTTGTATGTNNTCAGAGCACCCTTGGTGATTCCTTAAGCACTTAGCACAATTTGTAATCATACACCTATTTCTGCAATTATTTGTTGTAGAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGA SEQUENCE_INCLUDED_REGION=36,118 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.611000 PRIMER_LEFT_0_PENALTY=0.317911 PRIMER_RIGHT_0_PENALTY=2.293089 PRIMER_INTERNAL_0_PENALTY=1.052696 PRIMER_LEFT_0_SEQUENCE=GTCCCTGACTCCCAGACAAG PRIMER_RIGHT_0_SEQUENCE=GTGCTTAAGGAATCACCAAGG PRIMER_INTERNAL_0_SEQUENCE=GGCTGGGCCTTTTGTATGT PRIMER_LEFT_0=65,20 PRIMER_RIGHT_0=139,21 PRIMER_INTERNAL_0=89,19 PRIMER_LEFT_0_TM=59.682 PRIMER_RIGHT_0_TM=58.707 PRIMER_INTERNAL_0_TM=59.947 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_INTERNAL_0_GC_PERCENT=52.632 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=2.665040 PRIMER_LEFT_1_PENALTY=0.317911 PRIMER_RIGHT_1_PENALTY=2.347129 PRIMER_INTERNAL_1_PENALTY=1.052696 PRIMER_LEFT_1_SEQUENCE=GTCCCTGACTCCCAGACAAG PRIMER_RIGHT_1_SEQUENCE=AGTGCTTAAGGAATCACCAAGG PRIMER_INTERNAL_1_SEQUENCE=GGCTGGGCCTTTTGTATGT PRIMER_LEFT_1=65,20 PRIMER_RIGHT_1=140,22 PRIMER_INTERNAL_1=89,19 PRIMER_LEFT_1_TM=59.682 PRIMER_RIGHT_1_TM=59.653 PRIMER_INTERNAL_1_TM=59.947 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_INTERNAL_1_GC_PERCENT=52.632 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=3.511807 PRIMER_LEFT_2_PENALTY=0.317911 PRIMER_RIGHT_2_PENALTY=3.193896 PRIMER_INTERNAL_2_PENALTY=1.052696 PRIMER_LEFT_2_SEQUENCE=GTCCCTGACTCCCAGACAAG PRIMER_RIGHT_2_SEQUENCE=GCTAAGTGCTTAAGGAATCACCA PRIMER_INTERNAL_2_SEQUENCE=GGCTGGGCCTTTTGTATGT PRIMER_LEFT_2=65,20 PRIMER_RIGHT_2=144,23 PRIMER_INTERNAL_2=89,19 PRIMER_LEFT_2_TM=59.682 PRIMER_RIGHT_2_TM=59.806 PRIMER_INTERNAL_2_TM=59.947 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=43.478 PRIMER_INTERNAL_2_GC_PERCENT=52.632 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=3.511807 PRIMER_LEFT_3_PENALTY=0.317911 PRIMER_RIGHT_3_PENALTY=3.193896 PRIMER_INTERNAL_3_PENALTY=1.052696 PRIMER_LEFT_3_SEQUENCE=GTCCCTGACTCCCAGACAAG PRIMER_RIGHT_3_SEQUENCE=TGCTAAGTGCTTAAGGAATCACC PRIMER_INTERNAL_3_SEQUENCE=GGCTGGGCCTTTTGTATGT PRIMER_LEFT_3=65,20 PRIMER_RIGHT_3=145,23 PRIMER_INTERNAL_3=89,19 PRIMER_LEFT_3_TM=59.682 PRIMER_RIGHT_3_TM=59.806 PRIMER_INTERNAL_3_TM=59.947 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_INTERNAL_3_GC_PERCENT=52.632 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=81 PRIMER_PAIR_4_PENALTY=4.164937 PRIMER_LEFT_4_PENALTY=0.317911 PRIMER_RIGHT_4_PENALTY=3.847026 PRIMER_INTERNAL_4_PENALTY=1.052696 PRIMER_LEFT_4_SEQUENCE=GTCCCTGACTCCCAGACAAG PRIMER_RIGHT_4_SEQUENCE=AAGTGCTTAAGGAATCACCAAGG PRIMER_INTERNAL_4_SEQUENCE=GGCTGGGCCTTTTGTATGT PRIMER_LEFT_4=65,20 PRIMER_RIGHT_4=141,23 PRIMER_INTERNAL_4=89,19 PRIMER_LEFT_4_TM=59.682 PRIMER_RIGHT_4_TM=60.847 PRIMER_INTERNAL_4_TM=59.947 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_INTERNAL_4_GC_PERCENT=52.632 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=77 = SEQUENCE_ID=MH618 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAAAGCGTAAGAATACGTGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGNTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT SEQUENCE_INCLUDED_REGION=35,21 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH623 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCTTCATAGTNNGCGTNNTACAAAACAACTTAGTNTTCACATTCAATTCAGTAATTAACATGGTGTTTTNTTCCAGTGAACAGAAAAGTTATTATAAAACAATATATTTCCATTCGTCATTGTCTTTTCTCACTTCTGACTAGCCACAAGGTCTTGACAACTTTTTTTCTATGTAAAATGAGACTGTGCTATGAATATCTCAGAGG SEQUENCE_INCLUDED_REGION=34,206 P3_COMMENT=CL is ACATTCAATTCAGTAATTAACATGG P3_COMMENT=CR is TTCATAGCACAGTCTCATTTTACA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=8.948001 PRIMER_LEFT_0_PENALTY=2.461740 PRIMER_RIGHT_0_PENALTY=6.486261 PRIMER_INTERNAL_0_PENALTY=9.320856 PRIMER_LEFT_0_SEQUENCE=TCACTTCTGACTAGCCACAAGG PRIMER_RIGHT_0_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC PRIMER_INTERNAL_0_SEQUENCE=TGACAACTTTTTTTCTATGTAAAATGA PRIMER_LEFT_0=163,22 PRIMER_RIGHT_0=239,25 PRIMER_INTERNAL_0=188,27 PRIMER_LEFT_0_TM=59.538 PRIMER_RIGHT_0_TM=58.514 PRIMER_INTERNAL_0_TM=57.679 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=44.000 PRIMER_INTERNAL_0_GC_PERCENT=22.222 PRIMER_LEFT_0_SELF_ANY=10.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=8.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=9.419196 PRIMER_LEFT_1_PENALTY=2.932936 PRIMER_RIGHT_1_PENALTY=6.486261 PRIMER_INTERNAL_1_PENALTY=1.753359 PRIMER_LEFT_1_SEQUENCE=TTCCATTCGTCATTGTCTTTTC PRIMER_RIGHT_1_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC PRIMER_INTERNAL_1_SEQUENCE=GCCACAAGGTCTTGACAACTT PRIMER_LEFT_1=141,22 PRIMER_RIGHT_1=239,25 PRIMER_INTERNAL_1=176,21 PRIMER_LEFT_1_TM=59.067 PRIMER_RIGHT_1_TM=58.514 PRIMER_INTERNAL_1_TM=59.247 PRIMER_LEFT_1_GC_PERCENT=36.364 PRIMER_RIGHT_1_GC_PERCENT=44.000 PRIMER_INTERNAL_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=9.492569 PRIMER_LEFT_2_PENALTY=3.006308 PRIMER_RIGHT_2_PENALTY=6.486261 PRIMER_INTERNAL_2_PENALTY=1.753359 PRIMER_LEFT_2_SEQUENCE=TTCCATTCGTCATTGTCTTTTCT PRIMER_RIGHT_2_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC PRIMER_INTERNAL_2_SEQUENCE=GCCACAAGGTCTTGACAACTT PRIMER_LEFT_2=141,23 PRIMER_RIGHT_2=239,25 PRIMER_INTERNAL_2=176,21 PRIMER_LEFT_2_TM=59.994 PRIMER_RIGHT_2_TM=58.514 PRIMER_INTERNAL_2_TM=59.247 PRIMER_LEFT_2_GC_PERCENT=34.783 PRIMER_RIGHT_2_GC_PERCENT=44.000 PRIMER_INTERNAL_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=9.790787 PRIMER_LEFT_3_PENALTY=3.304526 PRIMER_RIGHT_3_PENALTY=6.486261 PRIMER_INTERNAL_3_PENALTY=1.753359 PRIMER_LEFT_3_SEQUENCE=CCATTCGTCATTGTCTTTTCTC PRIMER_RIGHT_3_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC PRIMER_INTERNAL_3_SEQUENCE=GCCACAAGGTCTTGACAACTT PRIMER_LEFT_3=143,22 PRIMER_RIGHT_3=239,25 PRIMER_INTERNAL_3=176,21 PRIMER_LEFT_3_TM=58.695 PRIMER_RIGHT_3_TM=58.514 PRIMER_INTERNAL_3_TM=59.247 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=44.000 PRIMER_INTERNAL_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=9.790787 PRIMER_LEFT_4_PENALTY=3.304526 PRIMER_RIGHT_4_PENALTY=6.486261 PRIMER_INTERNAL_4_PENALTY=1.753359 PRIMER_LEFT_4_SEQUENCE=TCCATTCGTCATTGTCTTTTCT PRIMER_RIGHT_4_SEQUENCE=CCTCTGAGATATTCATAGCACAGTC PRIMER_INTERNAL_4_SEQUENCE=GCCACAAGGTCTTGACAACTT PRIMER_LEFT_4=142,22 PRIMER_RIGHT_4=239,25 PRIMER_INTERNAL_4=176,21 PRIMER_LEFT_4_TM=58.695 PRIMER_RIGHT_4_TM=58.514 PRIMER_INTERNAL_4_TM=59.247 PRIMER_LEFT_4_GC_PERCENT=36.364 PRIMER_RIGHT_4_GC_PERCENT=44.000 PRIMER_INTERNAL_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH629 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTGCCCACAGCAGATGGTGACAGGGTCTACCAGGTAGTTCAAACAGATGACGCAGGTGAGTTCCTTCTGGAAGGCATGTGAGAAGTCTGAGTCCATTTTCCTAAGGAAAGAAANCCACAGGAATTTAATCTTCTACCCTGGAGAGACAAAGATCCAAGCAAAGTTTGAATCAGATCGTGATCGAATAATATCCTTTCTTTCTAAAGAAGTATAGGCTTTAATCTGCA SEQUENCE_INCLUDED_REGION=33,228 P3_COMMENT=CL is AACAGATGACGCAGGTGAGT P3_COMMENT=CR is AAGAAAGGATATTATTCGATCACG P3_COMMENT=CM is CCTTCTGGAAGGCATGTGAG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.917835 PRIMER_LEFT_0_PENALTY=0.286624 PRIMER_RIGHT_0_PENALTY=0.631211 PRIMER_INTERNAL_0_PENALTY=0.269335 PRIMER_LEFT_0_SEQUENCE=AGCAGATGGTGACAGGGTCT PRIMER_RIGHT_0_SEQUENCE=TTCTCACATGCCTTCCAGAA PRIMER_INTERNAL_0_SEQUENCE=AGATGACGCAGGTGAGTTCC PRIMER_LEFT_0=42,20 PRIMER_RIGHT_0=117,20 PRIMER_INTERNAL_0=78,20 PRIMER_LEFT_0_TM=59.713 PRIMER_RIGHT_0_TM=59.369 PRIMER_INTERNAL_0_TM=60.269 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=1.226796 PRIMER_LEFT_1_PENALTY=0.595585 PRIMER_RIGHT_1_PENALTY=0.631211 PRIMER_INTERNAL_1_PENALTY=0.269335 PRIMER_LEFT_1_SEQUENCE=ACAGCAGATGGTGACAGGGT PRIMER_RIGHT_1_SEQUENCE=TTCTCACATGCCTTCCAGAA PRIMER_INTERNAL_1_SEQUENCE=AGATGACGCAGGTGAGTTCC PRIMER_LEFT_1=40,20 PRIMER_RIGHT_1=117,20 PRIMER_INTERNAL_1=78,20 PRIMER_LEFT_1_TM=60.596 PRIMER_RIGHT_1_TM=59.369 PRIMER_INTERNAL_1_TM=60.269 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.1000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=1.329902 PRIMER_LEFT_2_PENALTY=0.286624 PRIMER_RIGHT_2_PENALTY=1.043279 PRIMER_INTERNAL_2_PENALTY=0.580148 PRIMER_LEFT_2_SEQUENCE=AGCAGATGGTGACAGGGTCT PRIMER_RIGHT_2_SEQUENCE=TCTCACATGCCTTCCAGAAG PRIMER_INTERNAL_2_SEQUENCE=CAGATGACGCAGGTGAGTTC PRIMER_LEFT_2=42,20 PRIMER_RIGHT_2=116,20 PRIMER_INTERNAL_2=77,20 PRIMER_LEFT_2_TM=59.713 PRIMER_RIGHT_2_TM=58.957 PRIMER_INTERNAL_2_TM=59.420 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.6000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=1.329902 PRIMER_LEFT_3_PENALTY=0.286624 PRIMER_RIGHT_3_PENALTY=1.043279 PRIMER_INTERNAL_3_PENALTY=0.269335 PRIMER_LEFT_3_SEQUENCE=AGCAGATGGTGACAGGGTCT PRIMER_RIGHT_3_SEQUENCE=CTTCTCACATGCCTTCCAGA PRIMER_INTERNAL_3_SEQUENCE=GATGACGCAGGTGAGTTCCT PRIMER_LEFT_3=42,20 PRIMER_RIGHT_3=118,20 PRIMER_INTERNAL_3=79,20 PRIMER_LEFT_3_TM=59.713 PRIMER_RIGHT_3_TM=58.957 PRIMER_INTERNAL_3_TM=60.269 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=77 PRIMER_PAIR_4_PENALTY=1.386172 PRIMER_LEFT_4_PENALTY=0.595585 PRIMER_RIGHT_4_PENALTY=0.790588 PRIMER_INTERNAL_4_PENALTY=0.707326 PRIMER_LEFT_4_SEQUENCE=ACAGCAGATGGTGACAGGGT PRIMER_RIGHT_4_SEQUENCE=CTCACATGCCTTCCAGAAGG PRIMER_INTERNAL_4_SEQUENCE=TTCAAACAGATGACGCAGGT PRIMER_LEFT_4=40,20 PRIMER_RIGHT_4=115,20 PRIMER_INTERNAL_4=71,20 PRIMER_LEFT_4_TM=60.596 PRIMER_RIGHT_4_TM=60.791 PRIMER_INTERNAL_4_TM=59.293 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=8.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.1000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH633 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCCAAAGAAAAGTGACTAAATCAAGAAGGTTAACTCACATCCCTGCCTGGGAACCGNGANGTCCTGGCCTATAGTAACAAAAATTTCTCTTTCATGCAATGTCTACTCTATTGCAGGCACAGGGCTAGGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATG SEQUENCE_INCLUDED_REGION=33,132 P3_COMMENT=CL is CTTCCAAAGAAAAGTGACTAAATC P3_COMMENT=CR is CTAGCCCTGTGCCTGCAATA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.866715 PRIMER_LEFT_0_PENALTY=0.589903 PRIMER_RIGHT_0_PENALTY=0.276812 PRIMER_INTERNAL_0_PENALTY=4.006288 PRIMER_LEFT_0_SEQUENCE=GGTTAACTCACATCCCTGCC PRIMER_RIGHT_0_SEQUENCE=CCCTGTGCCTGCAATAGAGT PRIMER_INTERNAL_0_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG PRIMER_LEFT_0=62,20 PRIMER_RIGHT_0=158,20 PRIMER_INTERNAL_0=112,24 PRIMER_LEFT_0_TM=59.410 PRIMER_RIGHT_0_TM=60.277 PRIMER_INTERNAL_0_TM=60.006 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=29.167 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=97 PRIMER_PAIR_1_PENALTY=1.331768 PRIMER_LEFT_1_PENALTY=1.054956 PRIMER_RIGHT_1_PENALTY=0.276812 PRIMER_INTERNAL_1_PENALTY=4.006288 PRIMER_LEFT_1_SEQUENCE=TAACTCACATCCCTGCCTGG PRIMER_RIGHT_1_SEQUENCE=CCCTGTGCCTGCAATAGAGT PRIMER_INTERNAL_1_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG PRIMER_LEFT_1=65,20 PRIMER_RIGHT_1=158,20 PRIMER_INTERNAL_1=112,24 PRIMER_LEFT_1_TM=61.055 PRIMER_RIGHT_1_TM=60.277 PRIMER_INTERNAL_1_TM=60.006 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=29.167 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=94 PRIMER_PAIR_2_PENALTY=1.554978 PRIMER_LEFT_2_PENALTY=1.278166 PRIMER_RIGHT_2_PENALTY=0.276812 PRIMER_INTERNAL_2_PENALTY=4.006288 PRIMER_LEFT_2_SEQUENCE=TTAACTCACATCCCTGCCTG PRIMER_RIGHT_2_SEQUENCE=CCCTGTGCCTGCAATAGAGT PRIMER_INTERNAL_2_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG PRIMER_LEFT_2=64,20 PRIMER_RIGHT_2=158,20 PRIMER_INTERNAL_2=112,24 PRIMER_LEFT_2_TM=58.722 PRIMER_RIGHT_2_TM=60.277 PRIMER_INTERNAL_2_TM=60.006 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=29.167 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=95 PRIMER_PAIR_3_PENALTY=1.652760 PRIMER_LEFT_3_PENALTY=1.375948 PRIMER_RIGHT_3_PENALTY=0.276812 PRIMER_INTERNAL_3_PENALTY=4.006288 PRIMER_LEFT_3_SEQUENCE=GGTTAACTCACATCCCTGCCT PRIMER_RIGHT_3_SEQUENCE=CCCTGTGCCTGCAATAGAGT PRIMER_INTERNAL_3_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG PRIMER_LEFT_3=62,21 PRIMER_RIGHT_3=158,20 PRIMER_INTERNAL_3=112,24 PRIMER_LEFT_3_TM=60.376 PRIMER_RIGHT_3_TM=60.277 PRIMER_INTERNAL_3_TM=60.006 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=29.167 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=1.652760 PRIMER_LEFT_4_PENALTY=1.375948 PRIMER_RIGHT_4_PENALTY=0.276812 PRIMER_INTERNAL_4_PENALTY=4.006288 PRIMER_LEFT_4_SEQUENCE=AGGTTAACTCACATCCCTGCC PRIMER_RIGHT_4_SEQUENCE=CCCTGTGCCTGCAATAGAGT PRIMER_INTERNAL_4_SEQUENCE=CAAAAATTTCTCTTTCATGCAATG PRIMER_LEFT_4=61,21 PRIMER_RIGHT_4=158,20 PRIMER_INTERNAL_4=112,24 PRIMER_LEFT_4_TM=60.376 PRIMER_RIGHT_4_TM=60.277 PRIMER_INTERNAL_4_TM=60.006 PRIMER_LEFT_4_GC_PERCENT=52.381 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=29.167 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=6.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH636 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTATCCCCCATCCCAGCCCACAACCATCTCACTGATGAGTCTGTAACATGAAACTAAATCAAAAAGATCCCCAACAGAATTAGAAAAAATATTAAACTCACTGCTTGCTTGTTGATTTAATCAACCTAGCCGGCTGTCATGTGGGATTAGAATAAAATAAACACAAAAATGAAAACACACGATTGCTAACAAAGCAGATTCTTTTTTCAAGGCACACGTAAAGATAATAACTTCAAAGTTGATTATATTAACTTGTCTTCCCATGATAACACG SEQUENCE_INCLUDED_REGION=36,276 P3_COMMENT=CL is ACTCACTGCTTGCTTGTTGA P3_COMMENT=CR is TTGAAGTTATTATCTTTACGTGTGC P3_COMMENT=CM is TTAATCAACCTAGCCGGCTG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.296956 PRIMER_LEFT_0_PENALTY=0.222749 PRIMER_RIGHT_0_PENALTY=1.074208 PRIMER_INTERNAL_0_PENALTY=7.771682 PRIMER_LEFT_0_SEQUENCE=TTAATCAACCTAGCCGGCTG PRIMER_RIGHT_0_SEQUENCE=TGCTTTGTTAGCAATCGTGTG PRIMER_INTERNAL_0_SEQUENCE=TGTGGGATTAGAATAAAATAAACACAA PRIMER_LEFT_0=155,20 PRIMER_RIGHT_0=234,21 PRIMER_INTERNAL_0=178,27 PRIMER_LEFT_0_TM=60.223 PRIMER_RIGHT_0_TM=59.926 PRIMER_INTERNAL_0_TM=59.228 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_INTERNAL_0_GC_PERCENT=25.926 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=1.344175 PRIMER_LEFT_1_PENALTY=0.269968 PRIMER_RIGHT_1_PENALTY=1.074208 PRIMER_INTERNAL_1_PENALTY=7.771682 PRIMER_LEFT_1_SEQUENCE=TAATCAACCTAGCCGGCTGT PRIMER_RIGHT_1_SEQUENCE=TGCTTTGTTAGCAATCGTGTG PRIMER_INTERNAL_1_SEQUENCE=TGTGGGATTAGAATAAAATAAACACAA PRIMER_LEFT_1=156,20 PRIMER_RIGHT_1=234,21 PRIMER_INTERNAL_1=178,27 PRIMER_LEFT_1_TM=59.730 PRIMER_RIGHT_1_TM=59.926 PRIMER_INTERNAL_1_TM=59.228 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_INTERNAL_1_GC_PERCENT=25.926 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=79 PRIMER_PAIR_2_PENALTY=1.690886 PRIMER_LEFT_2_PENALTY=1.468137 PRIMER_RIGHT_2_PENALTY=0.222749 PRIMER_INTERNAL_2_PENALTY=2.095684 PRIMER_LEFT_2_SEQUENCE=TCAAAAAGATCCCCAACAGAA PRIMER_RIGHT_2_SEQUENCE=CAGCCGGCTAGGTTGATTAA PRIMER_INTERNAL_2_SEQUENCE=AAACTCACTGCTTGCTTGTTGA PRIMER_LEFT_2=97,21 PRIMER_RIGHT_2=174,20 PRIMER_INTERNAL_2=132,22 PRIMER_LEFT_2_TM=59.532 PRIMER_RIGHT_2_TM=60.223 PRIMER_INTERNAL_2_TM=60.096 PRIMER_LEFT_2_GC_PERCENT=38.095 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=6.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=78 PRIMER_PAIR_3_PENALTY=1.738105 PRIMER_LEFT_3_PENALTY=1.468137 PRIMER_RIGHT_3_PENALTY=0.269968 PRIMER_INTERNAL_3_PENALTY=2.095684 PRIMER_LEFT_3_SEQUENCE=TCAAAAAGATCCCCAACAGAA PRIMER_RIGHT_3_SEQUENCE=ACAGCCGGCTAGGTTGATTA PRIMER_INTERNAL_3_SEQUENCE=AAACTCACTGCTTGCTTGTTGA PRIMER_LEFT_3=97,21 PRIMER_RIGHT_3=175,20 PRIMER_INTERNAL_3=132,22 PRIMER_LEFT_3_TM=59.532 PRIMER_RIGHT_3_TM=59.730 PRIMER_INTERNAL_3_TM=60.096 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=5.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=1.757691 PRIMER_LEFT_4_PENALTY=0.222749 PRIMER_RIGHT_4_PENALTY=1.534943 PRIMER_INTERNAL_4_PENALTY=2.725715 PRIMER_LEFT_4_SEQUENCE=TTAATCAACCTAGCCGGCTG PRIMER_RIGHT_4_SEQUENCE=TGCCTTGAAAAAAGAATCTGC PRIMER_INTERNAL_4_SEQUENCE=TGAAAACACACGATTGCTAACA PRIMER_LEFT_4=155,20 PRIMER_RIGHT_4=252,21 PRIMER_INTERNAL_4=208,22 PRIMER_LEFT_4_TM=60.223 PRIMER_RIGHT_4_TM=59.465 PRIMER_INTERNAL_4_TM=59.274 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_INTERNAL_4_GC_PERCENT=36.364 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH640 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTATAATCCACACACCTTGAATTAAATAGCACGTGAGAGGGNTCCTGGGGTAAATGAGCATTTAATCACTTTTCATACCCTTTTTATTGTCAAGGGAATGAACAGGGTCTGGAAAAGTGGTGAGGTATTTTGTTTTACTGCAGTTGCTATTTAGATTCATGGAAGTTTGAATAATGACTATTACTGAAAAAGATTGCTTTGGCCAGTTTAGATATTAAGAAAAAACCTGCAGAAAGATTATGG SEQUENCE_INCLUDED_REGION=35,242 P3_COMMENT=CL is AATTAAATAGCACGTGAGAGGG P3_COMMENT=CR is TTTTTTCTTAATATCTAAACTGGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.639098 PRIMER_LEFT_0_PENALTY=0.450843 PRIMER_RIGHT_0_PENALTY=0.188254 PRIMER_INTERNAL_0_PENALTY=4.330336 PRIMER_LEFT_0_SEQUENCE=GGTCTGGAAAAGTGGTGAGG PRIMER_RIGHT_0_SEQUENCE=GGCCAAAGCAATCTTTTTCA PRIMER_INTERNAL_0_SEQUENCE=GCAGTTGCTATTTAGATTCATGGA PRIMER_LEFT_0=139,20 PRIMER_RIGHT_0=237,20 PRIMER_INTERNAL_0=173,24 PRIMER_LEFT_0_TM=59.549 PRIMER_RIGHT_0_TM=60.188 PRIMER_INTERNAL_0_TM=59.670 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=37.500 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=99 PRIMER_PAIR_1_PENALTY=0.639098 PRIMER_LEFT_1_PENALTY=0.450843 PRIMER_RIGHT_1_PENALTY=0.188254 PRIMER_INTERNAL_1_PENALTY=4.330336 PRIMER_LEFT_1_SEQUENCE=GGTCTGGAAAAGTGGTGAGG PRIMER_RIGHT_1_SEQUENCE=TGGCCAAAGCAATCTTTTTC PRIMER_INTERNAL_1_SEQUENCE=GCAGTTGCTATTTAGATTCATGGA PRIMER_LEFT_1=139,20 PRIMER_RIGHT_1=238,20 PRIMER_INTERNAL_1=173,24 PRIMER_LEFT_1_TM=59.549 PRIMER_RIGHT_1_TM=60.188 PRIMER_INTERNAL_1_TM=59.670 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.3000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=0.639098 PRIMER_LEFT_2_PENALTY=0.450843 PRIMER_RIGHT_2_PENALTY=0.188254 PRIMER_INTERNAL_2_PENALTY=4.330336 PRIMER_LEFT_2_SEQUENCE=GGGTCTGGAAAAGTGGTGAG PRIMER_RIGHT_2_SEQUENCE=GGCCAAAGCAATCTTTTTCA PRIMER_INTERNAL_2_SEQUENCE=GCAGTTGCTATTTAGATTCATGGA PRIMER_LEFT_2=138,20 PRIMER_RIGHT_2=237,20 PRIMER_INTERNAL_2=173,24 PRIMER_LEFT_2_TM=59.549 PRIMER_RIGHT_2_TM=60.188 PRIMER_INTERNAL_2_TM=59.670 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.3000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=1.064062 PRIMER_LEFT_3_PENALTY=0.613219 PRIMER_RIGHT_3_PENALTY=0.450843 PRIMER_INTERNAL_3_PENALTY=2.899153 PRIMER_LEFT_3_SEQUENCE=TCCTGGGGTAAATGAGCATT PRIMER_RIGHT_3_SEQUENCE=TCACCACTTTTCCAGACCCT PRIMER_INTERNAL_3_SEQUENCE=CCCTTTTTATTGTCAAGGGAA PRIMER_LEFT_3=76,20 PRIMER_RIGHT_3=156,20 PRIMER_INTERNAL_3=111,21 PRIMER_LEFT_3_TM=59.387 PRIMER_RIGHT_3_TM=59.549 PRIMER_INTERNAL_3_TM=58.101 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.4000 PRIMER_RIGHT_3_END_STABILITY=9.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=81 PRIMER_PAIR_4_PENALTY=1.064062 PRIMER_LEFT_4_PENALTY=0.613219 PRIMER_RIGHT_4_PENALTY=0.450843 PRIMER_INTERNAL_4_PENALTY=2.899153 PRIMER_LEFT_4_SEQUENCE=TCCTGGGGTAAATGAGCATT PRIMER_RIGHT_4_SEQUENCE=CTCACCACTTTTCCAGACCC PRIMER_INTERNAL_4_SEQUENCE=CCCTTTTTATTGTCAAGGGAA PRIMER_LEFT_4=76,20 PRIMER_RIGHT_4=157,20 PRIMER_INTERNAL_4=111,21 PRIMER_LEFT_4_TM=59.387 PRIMER_RIGHT_4_TM=59.549 PRIMER_INTERNAL_4_TM=58.101 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=9.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH642 SEQUENCE_TEMPLATE=AACCTAAATTTTGCAAAGCAGTCTGGTCATATCGGAAAATATGCCTTTCTAGCAAAGCCTTGGTAAAATCAGAAGTGTTTCAAATTATGTCCTATTACTAAAGAGAACAAATTNTTACCCAACTTATGCAAATAACTGTATTGCCATGAGTTAAGAATACCCACAAATAGTTTCTCAATTTTGGAGGAATGAGGCAGAGAGAAATAGAAATTCTTCAGATTTGGCTAACAGAAGTATACTTTATCCAATGTTTTGTTAG SEQUENCE_INCLUDED_REGION=0,259 P3_COMMENT=CL is TGCAAAGCAGTCTGGTCATA P3_COMMENT=CR is CTGAAGAATTTCTATTTCTCTCTGC P3_COMMENT=CM is CGGAAAATATGCCTTTCTAGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.854017 PRIMER_LEFT_0_PENALTY=3.366081 PRIMER_RIGHT_0_PENALTY=0.487936 PRIMER_INTERNAL_0_PENALTY=7.324192 PRIMER_LEFT_0_SEQUENCE=TGCAAATAACTGTATTGCCATG PRIMER_RIGHT_0_SEQUENCE=CTCTCTGCCTCATTCCTCCA PRIMER_INTERNAL_0_SEQUENCE=AAGAATACCCACAAATAGTTTCTCAA PRIMER_LEFT_0=126,22 PRIMER_RIGHT_0=200,20 PRIMER_INTERNAL_0=152,26 PRIMER_LEFT_0_TM=58.634 PRIMER_RIGHT_0_TM=60.488 PRIMER_INTERNAL_0_TM=58.676 PRIMER_LEFT_0_GC_PERCENT=36.364 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=30.769 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=3.868756 PRIMER_LEFT_1_PENALTY=3.380820 PRIMER_RIGHT_1_PENALTY=0.487936 PRIMER_INTERNAL_1_PENALTY=7.324192 PRIMER_LEFT_1_SEQUENCE=TGCAAATAACTGTATTGCCATGA PRIMER_RIGHT_1_SEQUENCE=CTCTCTGCCTCATTCCTCCA PRIMER_INTERNAL_1_SEQUENCE=AAGAATACCCACAAATAGTTTCTCAA PRIMER_LEFT_1=126,23 PRIMER_RIGHT_1=200,20 PRIMER_INTERNAL_1=152,26 PRIMER_LEFT_1_TM=60.381 PRIMER_RIGHT_1_TM=60.488 PRIMER_INTERNAL_1_TM=58.676 PRIMER_LEFT_1_GC_PERCENT=34.783 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=30.769 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=3.880529 PRIMER_LEFT_2_PENALTY=3.366081 PRIMER_RIGHT_2_PENALTY=0.514448 PRIMER_INTERNAL_2_PENALTY=6.655237 PRIMER_LEFT_2_SEQUENCE=TGCAAATAACTGTATTGCCATG PRIMER_RIGHT_2_SEQUENCE=TCTCTCTGCCTCATTCCTCC PRIMER_INTERNAL_2_SEQUENCE=ACCCACAAATAGTTTCTCAATTTT PRIMER_LEFT_2=126,22 PRIMER_RIGHT_2=201,20 PRIMER_INTERNAL_2=158,24 PRIMER_LEFT_2_TM=58.634 PRIMER_RIGHT_2_TM=59.486 PRIMER_INTERNAL_2_TM=57.345 PRIMER_LEFT_2_GC_PERCENT=36.364 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=29.167 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=76 PRIMER_PAIR_3_PENALTY=3.895268 PRIMER_LEFT_3_PENALTY=3.380820 PRIMER_RIGHT_3_PENALTY=0.514448 PRIMER_INTERNAL_3_PENALTY=6.655237 PRIMER_LEFT_3_SEQUENCE=TGCAAATAACTGTATTGCCATGA PRIMER_RIGHT_3_SEQUENCE=TCTCTCTGCCTCATTCCTCC PRIMER_INTERNAL_3_SEQUENCE=ACCCACAAATAGTTTCTCAATTTT PRIMER_LEFT_3=126,23 PRIMER_RIGHT_3=201,20 PRIMER_INTERNAL_3=158,24 PRIMER_LEFT_3_TM=60.381 PRIMER_RIGHT_3_TM=59.486 PRIMER_INTERNAL_3_TM=57.345 PRIMER_LEFT_3_GC_PERCENT=34.783 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=29.167 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=4.536196 PRIMER_LEFT_4_PENALTY=4.048260 PRIMER_RIGHT_4_PENALTY=0.487936 PRIMER_INTERNAL_4_PENALTY=7.324192 PRIMER_LEFT_4_SEQUENCE=ATGCAAATAACTGTATTGCCATG PRIMER_RIGHT_4_SEQUENCE=CTCTCTGCCTCATTCCTCCA PRIMER_INTERNAL_4_SEQUENCE=AAGAATACCCACAAATAGTTTCTCAA PRIMER_LEFT_4=125,23 PRIMER_RIGHT_4=200,20 PRIMER_INTERNAL_4=152,26 PRIMER_LEFT_4_TM=58.952 PRIMER_RIGHT_4_TM=60.488 PRIMER_INTERNAL_4_TM=58.676 PRIMER_LEFT_4_GC_PERCENT=34.783 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=30.769 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH644 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTTGGTNAAACACTGTCTAGATATTGTTGTGAAGGCATTTTGTAGATGTAATTAACATGTTCAATTAGTTGACTTGAAGAAAAGGAGATAGTCTTTGTGGGTGAACCTCATCCAATCAGTNTAAGGTCTTCAGAGANAAAACTGCAGTTTCTCTGAGAAGAAGGACTTCTATCTTACGACTATAATATGGAAGAACTGCTTGAATTTCCATCTTGATGACCTGTCCTATGGATTTCAGACTTGTTAACCCCTGCAATCATCTGACCCAATGATACAGGAGTTAAAAAGAAATTATTTAGGCAGAGAGT SEQUENCE_INCLUDED_REGION=35,311 P3_COMMENT=CL is AAACACTGTCTAGATATTGTTGTGA P3_COMMENT=CR is CTCTCTGCCTAAATAATTTCTTTT P3_COMMENT=CM is AACATGTTCAATTAGTTGACTTGAA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.555001 PRIMER_LEFT_0_PENALTY=2.074231 PRIMER_RIGHT_0_PENALTY=0.480770 PRIMER_INTERNAL_0_PENALTY=3.129645 PRIMER_LEFT_0_SEQUENCE=TGCTTGAATTTCCATCTTGATG PRIMER_RIGHT_0_SEQUENCE=CATTGGGTCAGATGATTGCA PRIMER_INTERNAL_0_SEQUENCE=GGATTTCAGACTTGTTAACCCC PRIMER_LEFT_0=234,22 PRIMER_RIGHT_0=308,20 PRIMER_INTERNAL_0=267,22 PRIMER_LEFT_0_TM=60.074 PRIMER_RIGHT_0_TM=60.481 PRIMER_INTERNAL_0_TM=58.870 PRIMER_LEFT_0_GC_PERCENT=36.364 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=2.618211 PRIMER_LEFT_1_PENALTY=2.074231 PRIMER_RIGHT_1_PENALTY=0.543980 PRIMER_INTERNAL_1_PENALTY=3.129645 PRIMER_LEFT_1_SEQUENCE=TGCTTGAATTTCCATCTTGATG PRIMER_RIGHT_1_SEQUENCE=TCATTGGGTCAGATGATTGC PRIMER_INTERNAL_1_SEQUENCE=GGATTTCAGACTTGTTAACCCC PRIMER_LEFT_1=234,22 PRIMER_RIGHT_1=309,20 PRIMER_INTERNAL_1=267,22 PRIMER_LEFT_1_TM=60.074 PRIMER_RIGHT_1_TM=59.456 PRIMER_INTERNAL_1_TM=58.870 PRIMER_LEFT_1_GC_PERCENT=36.364 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=2.658942 PRIMER_LEFT_2_PENALTY=2.592680 PRIMER_RIGHT_2_PENALTY=0.066262 PRIMER_INTERNAL_2_PENALTY=2.449829 PRIMER_LEFT_2_SEQUENCE=GGAAGAACTGCTTGAATTTCCA PRIMER_RIGHT_2_SEQUENCE=GTCAGATGATTGCAGGGGTT PRIMER_INTERNAL_2_SEQUENCE=TCTTGATGACCTGTCCTATGGA PRIMER_LEFT_2=226,22 PRIMER_RIGHT_2=302,20 PRIMER_INTERNAL_2=248,22 PRIMER_LEFT_2_TM=60.593 PRIMER_RIGHT_2_TM=59.934 PRIMER_INTERNAL_2_TM=59.550 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=2.658942 PRIMER_LEFT_3_PENALTY=2.592680 PRIMER_RIGHT_3_PENALTY=0.066262 PRIMER_INTERNAL_3_PENALTY=2.449829 PRIMER_LEFT_3_SEQUENCE=TGGAAGAACTGCTTGAATTTCC PRIMER_RIGHT_3_SEQUENCE=GTCAGATGATTGCAGGGGTT PRIMER_INTERNAL_3_SEQUENCE=TCTTGATGACCTGTCCTATGGA PRIMER_LEFT_3=225,22 PRIMER_RIGHT_3=302,20 PRIMER_INTERNAL_3=248,22 PRIMER_LEFT_3_TM=60.593 PRIMER_RIGHT_3_TM=59.934 PRIMER_INTERNAL_3_TM=59.550 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=45.455 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=78 PRIMER_PAIR_4_PENALTY=3.073450 PRIMER_LEFT_4_PENALTY=2.592680 PRIMER_RIGHT_4_PENALTY=0.480770 PRIMER_INTERNAL_4_PENALTY=2.449829 PRIMER_LEFT_4_SEQUENCE=GGAAGAACTGCTTGAATTTCCA PRIMER_RIGHT_4_SEQUENCE=CATTGGGTCAGATGATTGCA PRIMER_INTERNAL_4_SEQUENCE=TCTTGATGACCTGTCCTATGGA PRIMER_LEFT_4=226,22 PRIMER_RIGHT_4=308,20 PRIMER_INTERNAL_4=248,22 PRIMER_LEFT_4_TM=60.593 PRIMER_RIGHT_4_TM=60.481 PRIMER_INTERNAL_4_TM=59.550 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.455 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=8.8000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=83 = SEQUENCE_ID=MH646 SEQUENCE_TEMPLATE=GCTNGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTGGAAGGAAGGACTGTGTTGTGTTCTCCGTTGCGTCCCCACCACGTAACACAGCAGCACTTAGCAGGTGCTTGGTCTGTATTTATTNAAAAATGNATNNATGNATATGTTGAATTAAATGNAAGTNAATTAAGTGAAAAGGATGAAACAGATGGAAGCTTCATGTTTCTTCCAAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCNGTGTGAAATTNTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT SEQUENCE_INCLUDED_REGION=36,179 P3_COMMENT=CL is CTGTGTTGTGTTCTCCGTTG P3_COMMENT=CR is TGAAGCTTCCATCTGTTTCA P3_COMMENT=CM is GTCCCCACCACGTAACACAG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.423963 PRIMER_LEFT_0_PENALTY=0.229181 PRIMER_RIGHT_0_PENALTY=0.194782 PRIMER_INTERNAL_0_PENALTY=1.277790 PRIMER_LEFT_0_SEQUENCE=TCCTGGAAGGAAGGACTGTG PRIMER_RIGHT_0_SEQUENCE=CAAGCACCTGCTAAGTGCTG PRIMER_INTERNAL_0_SEQUENCE=GTCCCCACCACGTAACACA PRIMER_LEFT_0=37,20 PRIMER_RIGHT_0=112,20 PRIMER_INTERNAL_0=73,19 PRIMER_LEFT_0_TM=60.229 PRIMER_RIGHT_0_TM=59.805 PRIMER_INTERNAL_0_TM=60.278 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=0.691018 PRIMER_LEFT_1_PENALTY=0.229181 PRIMER_RIGHT_1_PENALTY=0.461837 PRIMER_INTERNAL_1_PENALTY=1.277790 PRIMER_LEFT_1_SEQUENCE=TCCTGGAAGGAAGGACTGTG PRIMER_RIGHT_1_SEQUENCE=ACCAAGCACCTGCTAAGTGC PRIMER_INTERNAL_1_SEQUENCE=GTCCCCACCACGTAACACA PRIMER_LEFT_1=37,20 PRIMER_RIGHT_1=114,20 PRIMER_INTERNAL_1=73,19 PRIMER_LEFT_1_TM=60.229 PRIMER_RIGHT_1_TM=60.462 PRIMER_INTERNAL_1_TM=60.278 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=0.821413 PRIMER_LEFT_2_PENALTY=0.229181 PRIMER_RIGHT_2_PENALTY=0.592232 PRIMER_INTERNAL_2_PENALTY=1.277790 PRIMER_LEFT_2_SEQUENCE=TCCTGGAAGGAAGGACTGTG PRIMER_RIGHT_2_SEQUENCE=CCAAGCACCTGCTAAGTGCT PRIMER_INTERNAL_2_SEQUENCE=GTCCCCACCACGTAACACA PRIMER_LEFT_2=37,20 PRIMER_RIGHT_2=113,20 PRIMER_INTERNAL_2=73,19 PRIMER_LEFT_2_TM=60.229 PRIMER_RIGHT_2_TM=60.592 PRIMER_INTERNAL_2_TM=60.278 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=8.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=0.969455 PRIMER_LEFT_3_PENALTY=0.229181 PRIMER_RIGHT_3_PENALTY=0.740274 PRIMER_INTERNAL_3_PENALTY=1.277790 PRIMER_LEFT_3_SEQUENCE=TCCTGGAAGGAAGGACTGTG PRIMER_RIGHT_3_SEQUENCE=AAGCACCTGCTAAGTGCTGC PRIMER_INTERNAL_3_SEQUENCE=GTCCCCACCACGTAACACA PRIMER_LEFT_3=37,20 PRIMER_RIGHT_3=111,20 PRIMER_INTERNAL_3=73,19 PRIMER_LEFT_3_TM=60.229 PRIMER_RIGHT_3_TM=60.740 PRIMER_INTERNAL_3_TM=60.278 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=57.895 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=1.045598 PRIMER_LEFT_4_PENALTY=0.850816 PRIMER_RIGHT_4_PENALTY=0.194782 PRIMER_INTERNAL_4_PENALTY=1.277790 PRIMER_LEFT_4_SEQUENCE=CCTGGAAGGAAGGACTGTGT PRIMER_RIGHT_4_SEQUENCE=CAAGCACCTGCTAAGTGCTG PRIMER_INTERNAL_4_SEQUENCE=GTCCCCACCACGTAACACA PRIMER_LEFT_4=38,20 PRIMER_RIGHT_4=112,20 PRIMER_INTERNAL_4=73,19 PRIMER_LEFT_4_TM=59.149 PRIMER_RIGHT_4_TM=59.805 PRIMER_INTERNAL_4_TM=60.278 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=57.895 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH647 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTTGCCATATTATTAAAAGATATCCATCCTACTACTTTTTTACTATTATTTCACTTAACCAGATTGGAAAGAAATTTTATTTGATATCCGCAGCTCTAACCTAGCAATGGAATTTGAGTAATATGTTCCCTACAGCCATATTTTAATATGTAAAAAAGCTTATATCCCTGAAGTTCAATATTATGACACTATACACTATGTTATGACATATCTATTTTCTTAGAATGAAGTGANGAAAGGGAAGACTATGGTATTCTCAGAACTTGGGACTCAAACTCATATTATTGCAGGG SEQUENCE_INCLUDED_REGION=36,295 P3_COMMENT=CL is TGATATCCGCAGCTCTAACC P3_COMMENT=CR is CTGCAATAATATGAGTTTGAGTCC P3_COMMENT=CM is AGCAATGGAATTTGAGTAATATGTT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.808663 PRIMER_LEFT_0_PENALTY=0.668546 PRIMER_RIGHT_0_PENALTY=6.140118 PRIMER_INTERNAL_0_PENALTY=4.081412 PRIMER_LEFT_0_SEQUENCE=CCGCAGCTCTAACCTAGCAA PRIMER_RIGHT_0_SEQUENCE=TGAACTTCAGGGATATAAGCTTTTT PRIMER_INTERNAL_0_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA PRIMER_LEFT_0=126,20 PRIMER_RIGHT_0=215,25 PRIMER_INTERNAL_0=153,24 PRIMER_LEFT_0_TM=60.669 PRIMER_RIGHT_0_TM=58.860 PRIMER_INTERNAL_0_TM=59.919 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=90 PRIMER_PAIR_1_PENALTY=6.808663 PRIMER_LEFT_1_PENALTY=0.668546 PRIMER_RIGHT_1_PENALTY=6.140118 PRIMER_INTERNAL_1_PENALTY=4.081412 PRIMER_LEFT_1_SEQUENCE=CCGCAGCTCTAACCTAGCAA PRIMER_RIGHT_1_SEQUENCE=TTGAACTTCAGGGATATAAGCTTTT PRIMER_INTERNAL_1_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA PRIMER_LEFT_1=126,20 PRIMER_RIGHT_1=216,25 PRIMER_INTERNAL_1=153,24 PRIMER_LEFT_1_TM=60.669 PRIMER_RIGHT_1_TM=58.860 PRIMER_INTERNAL_1_TM=59.919 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=32.000 PRIMER_INTERNAL_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=7.3000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=6.990026 PRIMER_LEFT_2_PENALTY=0.668546 PRIMER_RIGHT_2_PENALTY=6.321480 PRIMER_INTERNAL_2_PENALTY=4.081412 PRIMER_LEFT_2_SEQUENCE=CCGCAGCTCTAACCTAGCAA PRIMER_RIGHT_2_SEQUENCE=TGAACTTCAGGGATATAAGCTTTT PRIMER_INTERNAL_2_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA PRIMER_LEFT_2=126,20 PRIMER_RIGHT_2=215,24 PRIMER_INTERNAL_2=153,24 PRIMER_LEFT_2_TM=60.669 PRIMER_RIGHT_2_TM=57.679 PRIMER_INTERNAL_2_TM=59.919 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=33.333 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=7.3000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=6.990026 PRIMER_LEFT_3_PENALTY=0.668546 PRIMER_RIGHT_3_PENALTY=6.321480 PRIMER_INTERNAL_3_PENALTY=4.081412 PRIMER_LEFT_3_SEQUENCE=CCGCAGCTCTAACCTAGCAA PRIMER_RIGHT_3_SEQUENCE=TTGAACTTCAGGGATATAAGCTTT PRIMER_INTERNAL_3_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA PRIMER_LEFT_3=126,20 PRIMER_RIGHT_3=216,24 PRIMER_INTERNAL_3=153,24 PRIMER_LEFT_3_TM=60.669 PRIMER_RIGHT_3_TM=57.679 PRIMER_INTERNAL_3_TM=59.919 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=91 PRIMER_PAIR_4_PENALTY=7.319712 PRIMER_LEFT_4_PENALTY=1.179594 PRIMER_RIGHT_4_PENALTY=6.140118 PRIMER_INTERNAL_4_PENALTY=4.081412 PRIMER_LEFT_4_SEQUENCE=CGCAGCTCTAACCTAGCAATG PRIMER_RIGHT_4_SEQUENCE=TGAACTTCAGGGATATAAGCTTTTT PRIMER_INTERNAL_4_SEQUENCE=TGAGTAATATGTTCCCTACAGCCA PRIMER_LEFT_4=127,21 PRIMER_RIGHT_4=215,25 PRIMER_INTERNAL_4=153,24 PRIMER_LEFT_4_TM=60.180 PRIMER_RIGHT_4_TM=58.860 PRIMER_INTERNAL_4_TM=59.919 PRIMER_LEFT_4_GC_PERCENT=52.381 PRIMER_RIGHT_4_GC_PERCENT=32.000 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH648 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCCAGTNATTTCAAGTGATTGCTGTGGGCATTCTATTTTAGGTNCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGAGAAACATTAGAGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAGGTAGGAAACATTATTCCCCTTTTTTAATTGCTTTGTTATTTGCTCGTTTAGTTGGATTTTGTGTACTTATTTGCAAATACATATTAGTTAGTGGCTTTTTGTCTTTTAAGAGAAGTTCTGCTCTACTTATGTAGGCAGTTTTCAACTCAGTTATCTCTTGAATTGAAAATAGGAGTAAAGGAAATTAAAATTTTCT SEQUENCE_INCLUDED_REGION=36,340 P3_COMMENT=CL is TTGCTGTGGGCATTCTATTT P3_COMMENT=CR is TCCTTTACTCCTATTTTCAATTCA P3_COMMENT=CM is CTGGTACTCAGGAATCATGGTG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.586707 PRIMER_LEFT_0_PENALTY=0.308722 PRIMER_RIGHT_0_PENALTY=0.277985 PRIMER_INTERNAL_0_PENALTY=2.527462 PRIMER_LEFT_0_SEQUENCE=TTTCAAGTGATTGCTGTGGG PRIMER_RIGHT_0_SEQUENCE=TGTTTCTCCAAGTGCAACCA PRIMER_INTERNAL_0_SEQUENCE=CTGGTACTCAGGAATCATGGTG PRIMER_LEFT_0=48,20 PRIMER_RIGHT_0=125,20 PRIMER_INTERNAL_0=84,22 PRIMER_LEFT_0_TM=59.691 PRIMER_RIGHT_0_TM=60.278 PRIMER_INTERNAL_0_TM=59.473 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 PRIMER_PAIR_1_PENALTY=1.292603 PRIMER_LEFT_1_PENALTY=1.014618 PRIMER_RIGHT_1_PENALTY=0.277985 PRIMER_INTERNAL_1_PENALTY=2.527462 PRIMER_LEFT_1_SEQUENCE=ATTTCAAGTGATTGCTGTGGG PRIMER_RIGHT_1_SEQUENCE=TGTTTCTCCAAGTGCAACCA PRIMER_INTERNAL_1_SEQUENCE=CTGGTACTCAGGAATCATGGTG PRIMER_LEFT_1=47,21 PRIMER_RIGHT_1=125,20 PRIMER_INTERNAL_1=84,22 PRIMER_LEFT_1_TM=59.985 PRIMER_RIGHT_1_TM=60.278 PRIMER_INTERNAL_1_TM=59.473 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=79 PRIMER_PAIR_2_PENALTY=1.852829 PRIMER_LEFT_2_PENALTY=0.308722 PRIMER_RIGHT_2_PENALTY=1.544107 PRIMER_INTERNAL_2_PENALTY=2.527462 PRIMER_LEFT_2_SEQUENCE=TTTCAAGTGATTGCTGTGGG PRIMER_RIGHT_2_SEQUENCE=ATGTTTCTCCAAGTGCAACCA PRIMER_INTERNAL_2_SEQUENCE=CTGGTACTCAGGAATCATGGTG PRIMER_LEFT_2=48,20 PRIMER_RIGHT_2=126,21 PRIMER_INTERNAL_2=84,22 PRIMER_LEFT_2_TM=59.691 PRIMER_RIGHT_2_TM=60.544 PRIMER_INTERNAL_2_TM=59.473 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=5.00 PRIMER_PAIR_2_PRODUCT_SIZE=79 PRIMER_PAIR_3_PENALTY=1.882221 PRIMER_LEFT_3_PENALTY=0.180577 PRIMER_RIGHT_3_PENALTY=1.701644 PRIMER_INTERNAL_3_PENALTY=2.274705 PRIMER_LEFT_3_SEQUENCE=GAATCATGGTGTGGTTGCAC PRIMER_RIGHT_3_SEQUENCE=TCCTACCTGAATCTCACAGGC PRIMER_INTERNAL_3_SEQUENCE=TTGGAGAAACATTAGAGGAGGC PRIMER_LEFT_3=95,20 PRIMER_RIGHT_3=185,21 PRIMER_INTERNAL_3=115,22 PRIMER_LEFT_3_TM=59.819 PRIMER_RIGHT_3_TM=59.298 PRIMER_INTERNAL_3_TM=59.725 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_INTERNAL_3_GC_PERCENT=45.455 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=91 PRIMER_PAIR_4_PENALTY=1.896048 PRIMER_LEFT_4_PENALTY=0.194404 PRIMER_RIGHT_4_PENALTY=1.701644 PRIMER_INTERNAL_4_PENALTY=2.274705 PRIMER_LEFT_4_SEQUENCE=CAGGAATCATGGTGTGGTTG PRIMER_RIGHT_4_SEQUENCE=TCCTACCTGAATCTCACAGGC PRIMER_INTERNAL_4_SEQUENCE=TTGGAGAAACATTAGAGGAGGC PRIMER_LEFT_4=92,20 PRIMER_RIGHT_4=185,21 PRIMER_INTERNAL_4=115,22 PRIMER_LEFT_4_TM=59.806 PRIMER_RIGHT_4_TM=59.298 PRIMER_INTERNAL_4_TM=59.725 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=45.455 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=94 = SEQUENCE_ID=MH650 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAACAGACCTGTGCCAAGGTTCACCTAACCTAGTACCTAACCTCATGTTTGTTTTACATGCATAAGACAATTCTGGGAAGATTAAAGTGTAAATTCCTAGTGGAGAATTTGAGTGGCCATTTTAATTATTTAATTAATTAATTTTGGTCCAGCACTCTTGTCTGATATTTTCTTCAGGTGACAGACTACACTTTTGAGAAACAATGGTGTTTTTCTTGTGCANCTCTGACTTGAATATACCAATCTGAACCGCTGCCAGTGGAGAATCTAACCACCTTCAAAATGATATCACTTTCATTCAATAATAAACAT SEQUENCE_INCLUDED_REGION=36,314 P3_COMMENT=CL is CTCAACAGACCTGTGCCAAG P3_COMMENT=CR is ATGAAAGTGATATCATTTTGAAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.404345 PRIMER_LEFT_0_PENALTY=0.022009 PRIMER_RIGHT_0_PENALTY=1.382337 PRIMER_INTERNAL_0_PENALTY=5.042469 PRIMER_LEFT_0_SEQUENCE=TGGTCCAGCACTCTTGTCTG PRIMER_RIGHT_0_SEQUENCE=TGCACAAGAAAAACACCATTG PRIMER_INTERNAL_0_SEQUENCE=TCAGGTGACAGACTACACTTTTGA PRIMER_LEFT_0=182,20 PRIMER_RIGHT_0=259,21 PRIMER_INTERNAL_0=211,24 PRIMER_LEFT_0_TM=60.022 PRIMER_RIGHT_0_TM=59.618 PRIMER_INTERNAL_0_TM=58.958 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 PRIMER_PAIR_1_PENALTY=2.198869 PRIMER_LEFT_1_PENALTY=0.158723 PRIMER_RIGHT_1_PENALTY=2.040146 PRIMER_INTERNAL_1_PENALTY=7.587579 PRIMER_LEFT_1_SEQUENCE=ACAGACCTGTGCCAAGGTTC PRIMER_RIGHT_1_SEQUENCE=TCCCAGAATTGTCTTATGCATG PRIMER_INTERNAL_1_SEQUENCE=CCTAGTACCTAACCTCATGTTTGTTTT PRIMER_LEFT_1=40,20 PRIMER_RIGHT_1=115,22 PRIMER_INTERNAL_1=66,27 PRIMER_LEFT_1_TM=60.159 PRIMER_RIGHT_1_TM=59.960 PRIMER_INTERNAL_1_TM=59.412 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_INTERNAL_1_GC_PERCENT=37.037 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=2.414734 PRIMER_LEFT_2_PENALTY=1.032397 PRIMER_RIGHT_2_PENALTY=1.382337 PRIMER_INTERNAL_2_PENALTY=5.042469 PRIMER_LEFT_2_SEQUENCE=GGTCCAGCACTCTTGTCTGA PRIMER_RIGHT_2_SEQUENCE=TGCACAAGAAAAACACCATTG PRIMER_INTERNAL_2_SEQUENCE=TCAGGTGACAGACTACACTTTTGA PRIMER_LEFT_2=183,20 PRIMER_RIGHT_2=259,21 PRIMER_INTERNAL_2=211,24 PRIMER_LEFT_2_TM=58.968 PRIMER_RIGHT_2_TM=59.618 PRIMER_INTERNAL_2_TM=58.958 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=38.095 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.2000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=2.417978 PRIMER_LEFT_3_PENALTY=0.377832 PRIMER_RIGHT_3_PENALTY=2.040146 PRIMER_INTERNAL_3_PENALTY=7.284315 PRIMER_LEFT_3_SEQUENCE=AACAGACCTGTGCCAAGGTT PRIMER_RIGHT_3_SEQUENCE=TCCCAGAATTGTCTTATGCATG PRIMER_INTERNAL_3_SEQUENCE=CACCTAACCTAGTACCTAACCTCATG PRIMER_LEFT_3=39,20 PRIMER_RIGHT_3=115,22 PRIMER_INTERNAL_3=59,26 PRIMER_LEFT_3_TM=59.622 PRIMER_RIGHT_3_TM=59.960 PRIMER_INTERNAL_3_TM=58.716 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=40.909 PRIMER_INTERNAL_3_GC_PERCENT=46.154 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=77 PRIMER_PAIR_4_PENALTY=2.462481 PRIMER_LEFT_4_PENALTY=0.022009 PRIMER_RIGHT_4_PENALTY=2.440473 PRIMER_INTERNAL_4_PENALTY=5.042469 PRIMER_LEFT_4_SEQUENCE=TGGTCCAGCACTCTTGTCTG PRIMER_RIGHT_4_SEQUENCE=TGCACAAGAAAAACACCATTGT PRIMER_INTERNAL_4_SEQUENCE=TCAGGTGACAGACTACACTTTTGA PRIMER_LEFT_4=182,20 PRIMER_RIGHT_4=259,22 PRIMER_INTERNAL_4=211,24 PRIMER_LEFT_4_TM=60.022 PRIMER_RIGHT_4_TM=60.440 PRIMER_INTERNAL_4_TM=58.958 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=36.364 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=78 = SEQUENCE_ID=MH652 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGGTNACAAGTNGAGGAAAGCAGGAAGGGAGTGAGAAGCCTGAAGCCTCTAGCCACCAGTTTCCCCAGGCAGTCCCCCATCCAAGCAACAGTCAGGCCTGACCTTGCTTAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG SEQUENCE_INCLUDED_REGION=36,111 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.480700 PRIMER_LEFT_0_PENALTY=0.182682 PRIMER_RIGHT_0_PENALTY=0.298018 PRIMER_INTERNAL_0_PENALTY=0.012470 PRIMER_LEFT_0_SEQUENCE=GAGGAAAGCAGGAAGGGAGT PRIMER_RIGHT_0_SEQUENCE=AAGGTCAGGCCTGACTGTTG PRIMER_INTERNAL_0_SEQUENCE=TGAAGCCTCTAGCCACCAGT PRIMER_LEFT_0=49,20 PRIMER_RIGHT_0=141,20 PRIMER_INTERNAL_0=77,20 PRIMER_LEFT_0_TM=59.817 PRIMER_RIGHT_0_TM=60.298 PRIMER_INTERNAL_0_TM=60.012 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=12.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=93 PRIMER_PAIR_1_PENALTY=0.636014 PRIMER_LEFT_1_PENALTY=0.182682 PRIMER_RIGHT_1_PENALTY=0.453332 PRIMER_INTERNAL_1_PENALTY=0.012470 PRIMER_LEFT_1_SEQUENCE=GAGGAAAGCAGGAAGGGAGT PRIMER_RIGHT_1_SEQUENCE=GTCAGGCCTGACTGTTGCTT PRIMER_INTERNAL_1_SEQUENCE=TGAAGCCTCTAGCCACCAGT PRIMER_LEFT_1=49,20 PRIMER_RIGHT_1=138,20 PRIMER_INTERNAL_1=77,20 PRIMER_LEFT_1_TM=59.817 PRIMER_RIGHT_1_TM=60.453 PRIMER_INTERNAL_1_TM=60.012 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=12.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=90 PRIMER_PAIR_2_PENALTY=0.768018 PRIMER_LEFT_2_PENALTY=0.470001 PRIMER_RIGHT_2_PENALTY=0.298018 PRIMER_INTERNAL_2_PENALTY=1.007677 PRIMER_LEFT_2_SEQUENCE=GAAGGGAGTGAGAAGCCTGA PRIMER_RIGHT_2_SEQUENCE=AAGGTCAGGCCTGACTGTTG PRIMER_INTERNAL_2_SEQUENCE=CTCTAGCCACCAGTTTCCCC PRIMER_LEFT_2=60,20 PRIMER_RIGHT_2=141,20 PRIMER_INTERNAL_2=83,20 PRIMER_LEFT_2_TM=59.530 PRIMER_RIGHT_2_TM=60.298 PRIMER_INTERNAL_2_TM=61.008 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=12.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=82 PRIMER_PAIR_3_PENALTY=0.859125 PRIMER_LEFT_3_PENALTY=0.561107 PRIMER_RIGHT_3_PENALTY=0.298018 PRIMER_INTERNAL_3_PENALTY=1.007677 PRIMER_LEFT_3_SEQUENCE=AGGAAGGGAGTGAGAAGCCT PRIMER_RIGHT_3_SEQUENCE=AAGGTCAGGCCTGACTGTTG PRIMER_INTERNAL_3_SEQUENCE=CTCTAGCCACCAGTTTCCCC PRIMER_LEFT_3=58,20 PRIMER_RIGHT_3=141,20 PRIMER_INTERNAL_3=83,20 PRIMER_LEFT_3_TM=59.439 PRIMER_RIGHT_3_TM=60.298 PRIMER_INTERNAL_3_TM=61.008 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=12.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=84 PRIMER_PAIR_4_PENALTY=0.923333 PRIMER_LEFT_4_PENALTY=0.470001 PRIMER_RIGHT_4_PENALTY=0.453332 PRIMER_INTERNAL_4_PENALTY=1.007677 PRIMER_LEFT_4_SEQUENCE=GAAGGGAGTGAGAAGCCTGA PRIMER_RIGHT_4_SEQUENCE=GTCAGGCCTGACTGTTGCTT PRIMER_INTERNAL_4_SEQUENCE=CTCTAGCCACCAGTTTCCCC PRIMER_LEFT_4=60,20 PRIMER_RIGHT_4=138,20 PRIMER_INTERNAL_4=83,20 PRIMER_LEFT_4_TM=59.530 PRIMER_RIGHT_4_TM=60.453 PRIMER_INTERNAL_4_TM=61.008 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=12.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=5.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH657 SEQUENCE_TEMPLATE=ANGNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGAACATGACCACCCTCCCCACCATTGCTGCTGGGATGAGCACCCAGACCAGGGCACCAGAAAGCCACGGAAGCCACAGCCACTAGCCCTGAACCTTGCACACCCCTGGAGTTTCTCTCCCCTCCCTATCCCCTCACCAACACCTTCCAGTGCTTATTCTGCTGTGTCAAAATGATCCTTCTGTTGCTGCACTGTCATTACTGTTGTATGGATTTATAATTATTGTCCAAAAAAGCCCCGAGCCTGGTACAGAAAAGGCATGGGGAAAGATGTGTCAGAATGCACAGTG SEQUENCE_INCLUDED_REGION=38,290 P3_COMMENT=CL is GACCAGGGCACCAGAAAG P3_COMMENT=CR is TCTGACACATCTTTCCCCAT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.075898 PRIMER_LEFT_0_PENALTY=0.008461 PRIMER_RIGHT_0_PENALTY=0.067436 PRIMER_INTERNAL_0_PENALTY=1.080628 PRIMER_LEFT_0_SEQUENCE=TGATCCTTCTGTTGCTGCAC PRIMER_RIGHT_0_SEQUENCE=TCCCCATGCCTTTTCTGTAC PRIMER_INTERNAL_0_SEQUENCE=TTATTGTCCAAAAAAGCCCC PRIMER_LEFT_0=211,20 PRIMER_RIGHT_0=304,20 PRIMER_INTERNAL_0=258,20 PRIMER_LEFT_0_TM=59.992 PRIMER_RIGHT_0_TM=59.933 PRIMER_INTERNAL_0_TM=58.919 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=5.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=94 PRIMER_PAIR_1_PENALTY=0.133117 PRIMER_LEFT_1_PENALTY=0.008461 PRIMER_RIGHT_1_PENALTY=0.124655 PRIMER_INTERNAL_1_PENALTY=1.080628 PRIMER_LEFT_1_SEQUENCE=TGATCCTTCTGTTGCTGCAC PRIMER_RIGHT_1_SEQUENCE=TGCCTTTTCTGTACCAGGCT PRIMER_INTERNAL_1_SEQUENCE=TTATTGTCCAAAAAAGCCCC PRIMER_LEFT_1=211,20 PRIMER_RIGHT_1=298,20 PRIMER_INTERNAL_1=258,20 PRIMER_LEFT_1_TM=59.992 PRIMER_RIGHT_1_TM=59.875 PRIMER_INTERNAL_1_TM=58.919 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=88 PRIMER_PAIR_2_PENALTY=0.139601 PRIMER_LEFT_2_PENALTY=0.008461 PRIMER_RIGHT_2_PENALTY=0.131140 PRIMER_INTERNAL_2_PENALTY=7.859678 PRIMER_LEFT_2_SEQUENCE=TGATCCTTCTGTTGCTGCAC PRIMER_RIGHT_2_SEQUENCE=CTTTTCTGTACCAGGCTCGG PRIMER_INTERNAL_2_SEQUENCE=TTTATAATTATTGTCCAAAAAAGCC PRIMER_LEFT_2=211,20 PRIMER_RIGHT_2=295,20 PRIMER_INTERNAL_2=251,25 PRIMER_LEFT_2_TM=59.992 PRIMER_RIGHT_2_TM=59.869 PRIMER_INTERNAL_2_TM=57.140 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=24.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=85 PRIMER_PAIR_3_PENALTY=0.139601 PRIMER_LEFT_3_PENALTY=0.008461 PRIMER_RIGHT_3_PENALTY=0.131140 PRIMER_INTERNAL_3_PENALTY=3.789337 PRIMER_LEFT_3_SEQUENCE=TGATCCTTCTGTTGCTGCAC PRIMER_RIGHT_3_SEQUENCE=CCTTTTCTGTACCAGGCTCG PRIMER_INTERNAL_3_SEQUENCE=AATTATTGTCCAAAAAAGCCC PRIMER_LEFT_3=211,20 PRIMER_RIGHT_3=296,20 PRIMER_INTERNAL_3=256,21 PRIMER_LEFT_3_TM=59.992 PRIMER_RIGHT_3_TM=59.869 PRIMER_INTERNAL_3_TM=57.211 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=33.333 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=9.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=86 PRIMER_PAIR_4_PENALTY=0.194404 PRIMER_LEFT_4_PENALTY=0.185942 PRIMER_RIGHT_4_PENALTY=0.008461 PRIMER_INTERNAL_4_PENALTY=0.001978 PRIMER_LEFT_4_SEQUENCE=CTGGAGTTTCTCTCCCCTCC PRIMER_RIGHT_4_SEQUENCE=GTGCAGCAACAGAAGGATCA PRIMER_INTERNAL_4_SEQUENCE=CCTCACCAACACCTTCCAGT PRIMER_LEFT_4=144,20 PRIMER_RIGHT_4=230,20 PRIMER_INTERNAL_4=170,20 PRIMER_LEFT_4_TM=60.186 PRIMER_RIGHT_4_TM=59.992 PRIMER_INTERNAL_4_TM=60.002 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH660 SEQUENCE_TEMPLATE=GCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCCTGGCCGCCCACCCCGCCCCGCTCCCTGCGAGGAGCGNAGNATCTCAGAAACCAGAAACGGCGCCCACCGCACCCCACACGGCCCCACCTGGGGCCCNTCCACGTGNATGTCCTCGCGGAACTGGGCCAGCGGGTAGACAAGTCGCAGAAGAGCGAGGAGTCCGCGGCGGGGAAGGCGGCGTCCCGGAACAG SEQUENCE_INCLUDED_REGION=32,197 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.198301 PRIMER_LEFT_0_PENALTY=2.381087 PRIMER_RIGHT_0_PENALTY=1.817214 PRIMER_INTERNAL_0_PENALTY=4.236162 PRIMER_LEFT_0_SEQUENCE=CAGAAACCAGAAACGGCG PRIMER_RIGHT_0_SEQUENCE=GACTTGTCTACCCGCTGGC PRIMER_INTERNAL_0_SEQUENCE=ATGTCCTCGCGGAACTGG PRIMER_LEFT_0=82,18 PRIMER_RIGHT_0=180,19 PRIMER_INTERNAL_0=144,18 PRIMER_LEFT_0_TM=60.381 PRIMER_RIGHT_0_TM=60.817 PRIMER_INTERNAL_0_TM=62.236 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_INTERNAL_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=13.4000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=99 PRIMER_PAIR_1_PENALTY=5.112432 PRIMER_LEFT_1_PENALTY=3.295218 PRIMER_RIGHT_1_PENALTY=1.817214 PRIMER_INTERNAL_1_PENALTY=4.236162 PRIMER_LEFT_1_SEQUENCE=TCAGAAACCAGAAACGGCG PRIMER_RIGHT_1_SEQUENCE=GACTTGTCTACCCGCTGGC PRIMER_INTERNAL_1_SEQUENCE=ATGTCCTCGCGGAACTGG PRIMER_LEFT_1=81,19 PRIMER_RIGHT_1=180,19 PRIMER_INTERNAL_1=144,18 PRIMER_LEFT_1_TM=62.295 PRIMER_RIGHT_1_TM=60.817 PRIMER_INTERNAL_1_TM=62.236 PRIMER_LEFT_1_GC_PERCENT=52.632 PRIMER_RIGHT_1_GC_PERCENT=63.158 PRIMER_INTERNAL_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=13.4000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=5.146012 PRIMER_LEFT_2_PENALTY=3.328798 PRIMER_RIGHT_2_PENALTY=1.817214 PRIMER_INTERNAL_2_PENALTY=4.236162 PRIMER_LEFT_2_SEQUENCE=AGAAACCAGAAACGGCGC PRIMER_RIGHT_2_SEQUENCE=GACTTGTCTACCCGCTGGC PRIMER_INTERNAL_2_SEQUENCE=ATGTCCTCGCGGAACTGG PRIMER_LEFT_2=83,18 PRIMER_RIGHT_2=180,19 PRIMER_INTERNAL_2=144,18 PRIMER_LEFT_2_TM=61.329 PRIMER_RIGHT_2_TM=60.817 PRIMER_INTERNAL_2_TM=62.236 PRIMER_LEFT_2_GC_PERCENT=55.556 PRIMER_RIGHT_2_GC_PERCENT=63.158 PRIMER_INTERNAL_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=12.9000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=98 PRIMER_PAIR_3_PENALTY=5.178689 PRIMER_LEFT_3_PENALTY=2.381087 PRIMER_RIGHT_3_PENALTY=2.797602 PRIMER_INTERNAL_3_PENALTY=4.236162 PRIMER_LEFT_3_SEQUENCE=CAGAAACCAGAAACGGCG PRIMER_RIGHT_3_SEQUENCE=CGACTTGTCTACCCGCTGG PRIMER_INTERNAL_3_SEQUENCE=ATGTCCTCGCGGAACTGG PRIMER_LEFT_3=82,18 PRIMER_RIGHT_3=181,19 PRIMER_INTERNAL_3=144,18 PRIMER_LEFT_3_TM=60.381 PRIMER_RIGHT_3_TM=61.798 PRIMER_INTERNAL_3_TM=62.236 PRIMER_LEFT_3_GC_PERCENT=55.556 PRIMER_RIGHT_3_GC_PERCENT=63.158 PRIMER_INTERNAL_3_GC_PERCENT=61.111 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=13.4000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=5.551363 PRIMER_LEFT_4_PENALTY=2.381087 PRIMER_RIGHT_4_PENALTY=3.170275 PRIMER_INTERNAL_4_PENALTY=4.236162 PRIMER_LEFT_4_SEQUENCE=CAGAAACCAGAAACGGCG PRIMER_RIGHT_4_SEQUENCE=ACTTGTCTACCCGCTGGC PRIMER_INTERNAL_4_SEQUENCE=ATGTCCTCGCGGAACTGG PRIMER_LEFT_4=82,18 PRIMER_RIGHT_4=179,18 PRIMER_INTERNAL_4=144,18 PRIMER_LEFT_4_TM=60.381 PRIMER_RIGHT_4_TM=58.830 PRIMER_INTERNAL_4_TM=62.236 PRIMER_LEFT_4_GC_PERCENT=55.556 PRIMER_RIGHT_4_GC_PERCENT=61.111 PRIMER_INTERNAL_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=13.4000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH661 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCCTCCCGGTGCCGCCCGCGCCTGCTCCTAGGTTCTTTTTAAGAGGTGTTTTAAAGGTGTTTCACTTTATACTAGAATAACACAAAGAAAATATTAAGAAGATTTAACAAGAATAAAATCATAATTAATTACAGGCATATCAGTGTCTGAGAAGATGATGTGTAGAAAACTGATTTGAGCAGGAATAGTCTGTATTACAGTTTCCTGATATGATTTTCTCGGTAACTGGGAGAACTAGACAGAAGATAAAGGTAATAGGGTTTA SEQUENCE_INCLUDED_REGION=36,265 P3_COMMENT=CL is GCCTGCTCCTAGGTTCTTTT P3_COMMENT=CR is CCCAGTTACCGAGAAAATCA P3_COMMENT=CM is AAGAGGTGTTTTAAAGGTGTTTCA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.426974 PRIMER_LEFT_0_PENALTY=3.981846 PRIMER_RIGHT_0_PENALTY=1.445128 PRIMER_INTERNAL_0_PENALTY=3.876179 PRIMER_LEFT_0_SEQUENCE=CAGGCATATCAGTGTCTGAGAAG PRIMER_RIGHT_0_SEQUENCE=TCCCAGTTACCGAGAAAATCA PRIMER_INTERNAL_0_SEQUENCE=AGAAAACTGATTTGAGCAGGAA PRIMER_LEFT_0=168,23 PRIMER_RIGHT_0=267,21 PRIMER_INTERNAL_0=200,22 PRIMER_LEFT_0_TM=59.018 PRIMER_RIGHT_0_TM=59.555 PRIMER_INTERNAL_0_TM=58.124 PRIMER_LEFT_0_GC_PERCENT=47.826 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_INTERNAL_0_GC_PERCENT=36.364 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=5.465596 PRIMER_LEFT_1_PENALTY=4.020468 PRIMER_RIGHT_1_PENALTY=1.445128 PRIMER_INTERNAL_1_PENALTY=3.876179 PRIMER_LEFT_1_SEQUENCE=CAGGCATATCAGTGTCTGAGAA PRIMER_RIGHT_1_SEQUENCE=TCCCAGTTACCGAGAAAATCA PRIMER_INTERNAL_1_SEQUENCE=AGAAAACTGATTTGAGCAGGAA PRIMER_LEFT_1=168,22 PRIMER_RIGHT_1=267,21 PRIMER_INTERNAL_1=200,22 PRIMER_LEFT_1_TM=57.980 PRIMER_RIGHT_1_TM=59.555 PRIMER_INTERNAL_1_TM=58.124 PRIMER_LEFT_1_GC_PERCENT=45.455 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_INTERNAL_1_GC_PERCENT=36.364 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=5.603425 PRIMER_LEFT_2_PENALTY=4.158297 PRIMER_RIGHT_2_PENALTY=1.445128 PRIMER_INTERNAL_2_PENALTY=3.876179 PRIMER_LEFT_2_SEQUENCE=GGCATATCAGTGTCTGAGAAGATG PRIMER_RIGHT_2_SEQUENCE=TCCCAGTTACCGAGAAAATCA PRIMER_INTERNAL_2_SEQUENCE=AGAAAACTGATTTGAGCAGGAA PRIMER_LEFT_2=170,24 PRIMER_RIGHT_2=267,21 PRIMER_INTERNAL_2=200,22 PRIMER_LEFT_2_TM=60.158 PRIMER_RIGHT_2_TM=59.555 PRIMER_INTERNAL_2_TM=58.124 PRIMER_LEFT_2_GC_PERCENT=45.833 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=36.364 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=98 PRIMER_PAIR_3_PENALTY=6.146485 PRIMER_LEFT_3_PENALTY=3.981846 PRIMER_RIGHT_3_PENALTY=2.164639 PRIMER_INTERNAL_3_PENALTY=3.876179 PRIMER_LEFT_3_SEQUENCE=CAGGCATATCAGTGTCTGAGAAG PRIMER_RIGHT_3_SEQUENCE=TCCCAGTTACCGAGAAAATCAT PRIMER_INTERNAL_3_SEQUENCE=AGAAAACTGATTTGAGCAGGAA PRIMER_LEFT_3=168,23 PRIMER_RIGHT_3=267,22 PRIMER_INTERNAL_3=200,22 PRIMER_LEFT_3_TM=59.018 PRIMER_RIGHT_3_TM=59.835 PRIMER_INTERNAL_3_TM=58.124 PRIMER_LEFT_3_GC_PERCENT=47.826 PRIMER_RIGHT_3_GC_PERCENT=40.909 PRIMER_INTERNAL_3_GC_PERCENT=36.364 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=6.185107 PRIMER_LEFT_4_PENALTY=4.020468 PRIMER_RIGHT_4_PENALTY=2.164639 PRIMER_INTERNAL_4_PENALTY=3.876179 PRIMER_LEFT_4_SEQUENCE=CAGGCATATCAGTGTCTGAGAA PRIMER_RIGHT_4_SEQUENCE=TCCCAGTTACCGAGAAAATCAT PRIMER_INTERNAL_4_SEQUENCE=AGAAAACTGATTTGAGCAGGAA PRIMER_LEFT_4=168,22 PRIMER_RIGHT_4=267,22 PRIMER_INTERNAL_4=200,22 PRIMER_LEFT_4_TM=57.980 PRIMER_RIGHT_4_TM=59.835 PRIMER_INTERNAL_4_TM=58.124 PRIMER_LEFT_4_GC_PERCENT=45.455 PRIMER_RIGHT_4_GC_PERCENT=40.909 PRIMER_INTERNAL_4_GC_PERCENT=36.364 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH665 SEQUENCE_TEMPLATE=GGAACACTATGTAGAGANACACAGGTGCAATTCCTNCTNCCCTTTGACCTTATAGTGAGTGNCCCTGCATGAGGGTCATGATAATCACACCAAAAATACCTAATTTCAACTTAAGTGAGAACTGTGAAAGCCTGACCCTAACTAGGAGGCCTGAAGTGGAATTTCTGAGAAG SEQUENCE_INCLUDED_REGION=0,172 P3_COMMENT=CL is CCCTTTGACCTTATAGTGAGTG P3_COMMENT=CR is TCTCAGAAATTCCACTTCAGG P3_COMMENT=CM is CCCTGCATGAGGGTCATG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.597570 PRIMER_LEFT_0_PENALTY=0.523704 PRIMER_RIGHT_0_PENALTY=0.073866 PRIMER_INTERNAL_0_PENALTY=0.296722 PRIMER_LEFT_0_SEQUENCE=CCTGCATGAGGGTCATGATA PRIMER_RIGHT_0_SEQUENCE=AATTCCACTTCAGGCCTCCT PRIMER_INTERNAL_0_SEQUENCE=GAACTGTGAAAGCCTGACCC PRIMER_LEFT_0=63,20 PRIMER_RIGHT_0=162,20 PRIMER_INTERNAL_0=118,20 PRIMER_LEFT_0_TM=59.476 PRIMER_RIGHT_0_TM=60.074 PRIMER_INTERNAL_0_TM=59.703 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=0.820426 PRIMER_LEFT_1_PENALTY=0.523704 PRIMER_RIGHT_1_PENALTY=0.296722 PRIMER_INTERNAL_1_PENALTY=6.072226 PRIMER_LEFT_1_SEQUENCE=CCTGCATGAGGGTCATGATA PRIMER_RIGHT_1_SEQUENCE=GGGTCAGGCTTTCACAGTTC PRIMER_INTERNAL_1_SEQUENCE=TCACACCAAAAATACCTAATTTCAA PRIMER_LEFT_1=63,20 PRIMER_RIGHT_1=137,20 PRIMER_INTERNAL_1=84,25 PRIMER_LEFT_1_TM=59.476 PRIMER_RIGHT_1_TM=59.703 PRIMER_INTERNAL_1_TM=58.928 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=28.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=5.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=1.099059 PRIMER_LEFT_2_PENALTY=0.523704 PRIMER_RIGHT_2_PENALTY=0.575355 PRIMER_INTERNAL_2_PENALTY=0.296722 PRIMER_LEFT_2_SEQUENCE=CCTGCATGAGGGTCATGATA PRIMER_RIGHT_2_SEQUENCE=TTCCACTTCAGGCCTCCTAG PRIMER_INTERNAL_2_SEQUENCE=GAACTGTGAAAGCCTGACCC PRIMER_LEFT_2=63,20 PRIMER_RIGHT_2=160,20 PRIMER_INTERNAL_2=118,20 PRIMER_LEFT_2_TM=59.476 PRIMER_RIGHT_2_TM=59.425 PRIMER_INTERNAL_2_TM=59.703 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=5.9000 PRIMER_RIGHT_2_END_STABILITY=7.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=98 PRIMER_PAIR_3_PENALTY=1.605240 PRIMER_LEFT_3_PENALTY=0.523704 PRIMER_RIGHT_3_PENALTY=1.081536 PRIMER_INTERNAL_3_PENALTY=0.296722 PRIMER_LEFT_3_SEQUENCE=CCTGCATGAGGGTCATGATA PRIMER_RIGHT_3_SEQUENCE=AATTCCACTTCAGGCCTCCTA PRIMER_INTERNAL_3_SEQUENCE=GAACTGTGAAAGCCTGACCC PRIMER_LEFT_3=63,20 PRIMER_RIGHT_3=162,21 PRIMER_INTERNAL_3=118,20 PRIMER_LEFT_3_TM=59.476 PRIMER_RIGHT_3_TM=60.082 PRIMER_INTERNAL_3_TM=59.703 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=5.9000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=1.632216 PRIMER_LEFT_4_PENALTY=0.523704 PRIMER_RIGHT_4_PENALTY=1.108512 PRIMER_INTERNAL_4_PENALTY=0.296722 PRIMER_LEFT_4_SEQUENCE=CCTGCATGAGGGTCATGATA PRIMER_RIGHT_4_SEQUENCE=TCCACTTCAGGCCTCCTAGT PRIMER_INTERNAL_4_SEQUENCE=GAACTGTGAAAGCCTGACCC PRIMER_LEFT_4=63,20 PRIMER_RIGHT_4=159,20 PRIMER_INTERNAL_4=118,20 PRIMER_LEFT_4_TM=59.476 PRIMER_RIGHT_4_TM=58.891 PRIMER_INTERNAL_4_TM=59.703 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=5.9000 PRIMER_RIGHT_4_END_STABILITY=5.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=97 = SEQUENCE_ID=MH670 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGTTACGATTCTTTGCACTAAAACATTTTTATGTATCTCATACTTAGGTATTGGCATGTTTATGTTTATAAAACTTCTGATAAATGTTATACATGTCATGAAATTTAAGGGTGAAATATACATACATCTGAAAGTAGTGCCAAAAAACTGAAAATATATTTGCACACTTAAATGTCTTTGTTTCTAGCGATATTCATCTTCAACCATAATAGCCTTTTCTGAAGTGGTTTGTTTGGTTTTTATATTAGGTTTTCGTTGTCAGTGTTCCTTTCTAATTG SEQUENCE_INCLUDED_REGION=36,281 P3_COMMENT=CL is ATCTCATACTTAGGTATTGGCATG P3_COMMENT=CR is ATTAGAAAGGAACACTGACAACG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.194254 PRIMER_LEFT_0_PENALTY=3.681214 PRIMER_RIGHT_0_PENALTY=1.513039 PRIMER_INTERNAL_0_PENALTY=2.325733 PRIMER_LEFT_0_SEQUENCE=AGCGATATTCATCTTCAACCA PRIMER_RIGHT_0_SEQUENCE=GGAACACTGACAACGAAAACC PRIMER_INTERNAL_0_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT PRIMER_LEFT_0=224,21 PRIMER_RIGHT_0=306,21 PRIMER_INTERNAL_0=250,22 PRIMER_LEFT_0_TM=57.319 PRIMER_RIGHT_0_TM=59.487 PRIMER_INTERNAL_0_TM=59.674 PRIMER_LEFT_0_GC_PERCENT=38.095 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=83 PRIMER_PAIR_1_PENALTY=5.628046 PRIMER_LEFT_1_PENALTY=4.115007 PRIMER_RIGHT_1_PENALTY=1.513039 PRIMER_INTERNAL_1_PENALTY=2.325733 PRIMER_LEFT_1_SEQUENCE=TCTAGCGATATTCATCTTCAACCA PRIMER_RIGHT_1_SEQUENCE=GGAACACTGACAACGAAAACC PRIMER_INTERNAL_1_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT PRIMER_LEFT_1=221,24 PRIMER_RIGHT_1=306,21 PRIMER_INTERNAL_1=250,22 PRIMER_LEFT_1_TM=60.115 PRIMER_RIGHT_1_TM=59.487 PRIMER_INTERNAL_1_TM=59.674 PRIMER_LEFT_1_GC_PERCENT=37.500 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=86 PRIMER_PAIR_2_PENALTY=5.794336 PRIMER_LEFT_2_PENALTY=4.281296 PRIMER_RIGHT_2_PENALTY=1.513039 PRIMER_INTERNAL_2_PENALTY=2.325733 PRIMER_LEFT_2_SEQUENCE=AGCGATATTCATCTTCAACCAT PRIMER_RIGHT_2_SEQUENCE=GGAACACTGACAACGAAAACC PRIMER_INTERNAL_2_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT PRIMER_LEFT_2=224,22 PRIMER_RIGHT_2=306,21 PRIMER_INTERNAL_2=250,22 PRIMER_LEFT_2_TM=57.719 PRIMER_RIGHT_2_TM=59.487 PRIMER_INTERNAL_2_TM=59.674 PRIMER_LEFT_2_GC_PERCENT=36.364 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.8000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=6.084693 PRIMER_LEFT_3_PENALTY=4.571654 PRIMER_RIGHT_3_PENALTY=1.513039 PRIMER_INTERNAL_3_PENALTY=2.325733 PRIMER_LEFT_3_SEQUENCE=TAGCGATATTCATCTTCAACCA PRIMER_RIGHT_3_SEQUENCE=GGAACACTGACAACGAAAACC PRIMER_INTERNAL_3_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT PRIMER_LEFT_3=223,22 PRIMER_RIGHT_3=306,21 PRIMER_INTERNAL_3=250,22 PRIMER_LEFT_3_TM=57.428 PRIMER_RIGHT_3_TM=59.487 PRIMER_INTERNAL_3_TM=59.674 PRIMER_LEFT_3_GC_PERCENT=36.364 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=84 PRIMER_PAIR_4_PENALTY=6.091405 PRIMER_LEFT_4_PENALTY=4.578365 PRIMER_RIGHT_4_PENALTY=1.513039 PRIMER_INTERNAL_4_PENALTY=2.325733 PRIMER_LEFT_4_SEQUENCE=CTAGCGATATTCATCTTCAACCA PRIMER_RIGHT_4_SEQUENCE=GGAACACTGACAACGAAAACC PRIMER_INTERNAL_4_SEQUENCE=GCCTTTTCTGAAGTGGTTTGTT PRIMER_LEFT_4=222,23 PRIMER_RIGHT_4=306,21 PRIMER_INTERNAL_4=250,22 PRIMER_LEFT_4_TM=58.422 PRIMER_RIGHT_4_TM=59.487 PRIMER_INTERNAL_4_TM=59.674 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=85 = SEQUENCE_ID=MH672 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTACCTTGCCAAATGTTCCATTATAAAAGTTATAGTATCAATGATAGAAACTTTAGGAAATTTAAGATGGCGGACATCCTTACAATTTTCTTTGTAAGTACATTTAAAAAAGATGTCTGTGTACTTCAATTGGAAGGGAATGAGGGTTGACTCAAAGCATATAATTAATTATCCTTGGCTATTTTACACTGTGGCTAATTCAGNTGTTACTCTCTCAGGAAGACAGGGAGAAACTTGATTTATTTTGTCTGGAATCCTATTCTTATTAAAAA SEQUENCE_INCLUDED_REGION=36,273 P3_COMMENT=CL is TACCTTGCCAAATGTTCCAT P3_COMMENT=CR is TTCTCCCTGTCTTCCTGAGA P3_COMMENT=CM is GAAACTTTAGGAAATTTAAGATGGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.427758 PRIMER_LEFT_0_PENALTY=0.330865 PRIMER_RIGHT_0_PENALTY=0.096893 PRIMER_INTERNAL_0_PENALTY=5.825088 PRIMER_LEFT_0_SEQUENCE=TGGCGGACATCCTTACAATT PRIMER_RIGHT_0_SEQUENCE=TTGAGTCAACCCTCATTCCC PRIMER_INTERNAL_0_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA PRIMER_LEFT_0=104,20 PRIMER_RIGHT_0=191,20 PRIMER_INTERNAL_0=148,24 PRIMER_LEFT_0_TM=60.331 PRIMER_RIGHT_0_TM=59.903 PRIMER_INTERNAL_0_TM=58.175 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=37.500 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 PRIMER_PAIR_1_PENALTY=0.568945 PRIMER_LEFT_1_PENALTY=0.472052 PRIMER_RIGHT_1_PENALTY=0.096893 PRIMER_INTERNAL_1_PENALTY=5.825088 PRIMER_LEFT_1_SEQUENCE=TTAAGATGGCGGACATCCTT PRIMER_RIGHT_1_SEQUENCE=TTGAGTCAACCCTCATTCCC PRIMER_INTERNAL_1_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=191,20 PRIMER_INTERNAL_1=148,24 PRIMER_LEFT_1_TM=59.528 PRIMER_RIGHT_1_TM=59.903 PRIMER_INTERNAL_1_TM=58.175 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=7.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=94 PRIMER_PAIR_2_PENALTY=0.568945 PRIMER_LEFT_2_PENALTY=0.472052 PRIMER_RIGHT_2_PENALTY=0.096893 PRIMER_INTERNAL_2_PENALTY=5.825088 PRIMER_LEFT_2_SEQUENCE=TTTAAGATGGCGGACATCCT PRIMER_RIGHT_2_SEQUENCE=TTGAGTCAACCCTCATTCCC PRIMER_INTERNAL_2_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA PRIMER_LEFT_2=97,20 PRIMER_RIGHT_2=191,20 PRIMER_INTERNAL_2=148,24 PRIMER_LEFT_2_TM=59.528 PRIMER_RIGHT_2_TM=59.903 PRIMER_INTERNAL_2_TM=58.175 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=7.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=7.8000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=95 PRIMER_PAIR_3_PENALTY=0.579555 PRIMER_LEFT_3_PENALTY=0.482662 PRIMER_RIGHT_3_PENALTY=0.096893 PRIMER_INTERNAL_3_PENALTY=5.825088 PRIMER_LEFT_3_SEQUENCE=AGATGGCGGACATCCTTACA PRIMER_RIGHT_3_SEQUENCE=TTGAGTCAACCCTCATTCCC PRIMER_INTERNAL_3_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA PRIMER_LEFT_3=101,20 PRIMER_RIGHT_3=191,20 PRIMER_INTERNAL_3=148,24 PRIMER_LEFT_3_TM=60.483 PRIMER_RIGHT_3_TM=59.903 PRIMER_INTERNAL_3_TM=58.175 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=37.500 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=6.0000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=91 PRIMER_PAIR_4_PENALTY=0.824197 PRIMER_LEFT_4_PENALTY=0.727304 PRIMER_RIGHT_4_PENALTY=0.096893 PRIMER_INTERNAL_4_PENALTY=5.825088 PRIMER_LEFT_4_SEQUENCE=ATGGCGGACATCCTTACAAT PRIMER_RIGHT_4_SEQUENCE=TTGAGTCAACCCTCATTCCC PRIMER_INTERNAL_4_SEQUENCE=GATGTCTGTGTACTTCAATTGGAA PRIMER_LEFT_4=103,20 PRIMER_RIGHT_4=191,20 PRIMER_INTERNAL_4=148,24 PRIMER_LEFT_4_TM=59.273 PRIMER_RIGHT_4_TM=59.903 PRIMER_INTERNAL_4_TM=58.175 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=37.500 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH679 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCACAGTCTCCTTTCCTTCTGCTCAGTACATTGAAGACGTAGTTTCTTTCATTTTGAGGACTTCTCTTAAGCAAAATATTTGAGGACTAACTAATAAATATTTAGGAAAAACTTGTCTCAGTACAAGTGGACATTTTTGTNATTTCTTTGGTCCTCACTCCAACCATCATTTCCCCCTTCTATGCCATTCATCTTTAACAAAATAAGTTTTCCTGTTGCCAGGGAGGTAAAACTTTCACTCTACCCTCTTAGGTTCAGTATTCGGGGGATTGCAAATTAAACCAACAAAACACAGCTCCACCCCATCAACATCTGGGG SEQUENCE_INCLUDED_REGION=36,319 P3_COMMENT=CL is TGCACAGTCTCCTTTCCTTC P3_COMMENT=CR is GGAGCTGTGTTTTGTTGGTT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.041723 PRIMER_LEFT_0_PENALTY=0.029253 PRIMER_RIGHT_0_PENALTY=0.012470 PRIMER_INTERNAL_0_PENALTY=0.017342 PRIMER_LEFT_0_SEQUENCE=TGTTGCCAGGGAGGTAAAAC PRIMER_RIGHT_0_SEQUENCE=GGGTGGAGCTGTGTTTTGTT PRIMER_INTERNAL_0_SEQUENCE=GGTTCAGTATTCGGGGGATT PRIMER_LEFT_0=250,20 PRIMER_RIGHT_0=339,20 PRIMER_INTERNAL_0=288,20 PRIMER_LEFT_0_TM=59.971 PRIMER_RIGHT_0_TM=60.012 PRIMER_INTERNAL_0_TM=60.017 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=90 PRIMER_PAIR_1_PENALTY=0.115494 PRIMER_LEFT_1_PENALTY=0.103024 PRIMER_RIGHT_1_PENALTY=0.012470 PRIMER_INTERNAL_1_PENALTY=0.017342 PRIMER_LEFT_1_SEQUENCE=CTGTTGCCAGGGAGGTAAAA PRIMER_RIGHT_1_SEQUENCE=GGGTGGAGCTGTGTTTTGTT PRIMER_INTERNAL_1_SEQUENCE=GGTTCAGTATTCGGGGGATT PRIMER_LEFT_1=249,20 PRIMER_RIGHT_1=339,20 PRIMER_INTERNAL_1=288,20 PRIMER_LEFT_1_TM=60.103 PRIMER_RIGHT_1_TM=60.012 PRIMER_INTERNAL_1_TM=60.017 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=0.201877 PRIMER_LEFT_2_PENALTY=0.172624 PRIMER_RIGHT_2_PENALTY=0.029253 PRIMER_INTERNAL_2_PENALTY=5.490789 PRIMER_LEFT_2_SEQUENCE=CACTCCAACCATCATTTCCC PRIMER_RIGHT_2_SEQUENCE=GTTTTACCTCCCTGGCAACA PRIMER_INTERNAL_2_SEQUENCE=CCTTCTATGCCATTCATCTTTAA PRIMER_LEFT_2=192,20 PRIMER_RIGHT_2=269,20 PRIMER_INTERNAL_2=212,23 PRIMER_LEFT_2_TM=60.173 PRIMER_RIGHT_2_TM=59.971 PRIMER_INTERNAL_2_TM=57.509 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=34.783 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=78 PRIMER_PAIR_3_PENALTY=0.275648 PRIMER_LEFT_3_PENALTY=0.172624 PRIMER_RIGHT_3_PENALTY=0.103024 PRIMER_INTERNAL_3_PENALTY=5.490789 PRIMER_LEFT_3_SEQUENCE=CACTCCAACCATCATTTCCC PRIMER_RIGHT_3_SEQUENCE=TTTTACCTCCCTGGCAACAG PRIMER_INTERNAL_3_SEQUENCE=CCTTCTATGCCATTCATCTTTAA PRIMER_LEFT_3=192,20 PRIMER_RIGHT_3=268,20 PRIMER_INTERNAL_3=212,23 PRIMER_LEFT_3_TM=60.173 PRIMER_RIGHT_3_TM=60.103 PRIMER_INTERNAL_3_TM=57.509 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=34.783 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=77 PRIMER_PAIR_4_PENALTY=0.392242 PRIMER_LEFT_4_PENALTY=0.362989 PRIMER_RIGHT_4_PENALTY=0.029253 PRIMER_INTERNAL_4_PENALTY=0.372603 PRIMER_LEFT_4_SEQUENCE=TTGGTCCTCACTCCAACCAT PRIMER_RIGHT_4_SEQUENCE=GTTTTACCTCCCTGGCAACA PRIMER_INTERNAL_4_SEQUENCE=ATTTCCCCCTTCTATGCCAT PRIMER_LEFT_4=184,20 PRIMER_RIGHT_4=269,20 PRIMER_INTERNAL_4=205,20 PRIMER_LEFT_4_TM=60.363 PRIMER_RIGHT_4_TM=59.971 PRIMER_INTERNAL_4_TM=59.627 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=7.8000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH683 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTTCAATGAAACAAGACATTAGGAAAGAGAATGCAGAAAAAGTGTTACTTTGTCTACTTTAAATTTTCAAGTTGTACATTATCTATTCTCGTTTATCTCTGGGTNAATGACGATACCTTTGTCAGGGTGCGGACAGTAGGATAATAAGTAAGTTGTAAAGCCCAGTACACTTCTCTTGGCACTGAAGCAAATCAATCCCCTGAAATACACTGAAGACAATGGATGATCATTAAGATTTTCAGACAGGGTTTAAACGCATTAAAATGTGAAAA SEQUENCE_INCLUDED_REGION=35,275 P3_COMMENT=CL is CAAGACATTAGGAAAGAGAATGC P3_COMMENT=CR is GCGTTTAAACCCTGTCTGAA P3_COMMENT=CM is TTATCTATTCTCGTTTATCTCTGGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.058439 PRIMER_LEFT_0_PENALTY=0.044832 PRIMER_RIGHT_0_PENALTY=0.013607 PRIMER_INTERNAL_0_PENALTY=1.297410 PRIMER_LEFT_0_SEQUENCE=GGGTGCGGACAGTAGGATAA PRIMER_RIGHT_0_SEQUENCE=TCAGGGGATTGATTTGCTTC PRIMER_INTERNAL_0_SEQUENCE=CCCAGTACACTTCTCTTGGCA PRIMER_LEFT_0=162,20 PRIMER_RIGHT_0=240,20 PRIMER_INTERNAL_0=198,21 PRIMER_LEFT_0_TM=59.955 PRIMER_RIGHT_0_TM=60.014 PRIMER_INTERNAL_0_TM=60.297 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=2.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=79 PRIMER_PAIR_1_PENALTY=0.439693 PRIMER_LEFT_1_PENALTY=0.426086 PRIMER_RIGHT_1_PENALTY=0.013607 PRIMER_INTERNAL_1_PENALTY=1.297410 PRIMER_LEFT_1_SEQUENCE=AGGGTGCGGACAGTAGGATA PRIMER_RIGHT_1_SEQUENCE=TCAGGGGATTGATTTGCTTC PRIMER_INTERNAL_1_SEQUENCE=CCCAGTACACTTCTCTTGGCA PRIMER_LEFT_1=161,20 PRIMER_RIGHT_1=240,20 PRIMER_INTERNAL_1=198,21 PRIMER_LEFT_1_TM=59.574 PRIMER_RIGHT_1_TM=60.014 PRIMER_INTERNAL_1_TM=60.297 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.1000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=2.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=80 PRIMER_PAIR_2_PENALTY=0.528743 PRIMER_LEFT_2_PENALTY=0.516683 PRIMER_RIGHT_2_PENALTY=0.012060 PRIMER_INTERNAL_2_PENALTY=5.952353 PRIMER_LEFT_2_SEQUENCE=GATACCTTTGTCAGGGTGCG PRIMER_RIGHT_2_SEQUENCE=TGATTTGCTTCAGTGCCAAG PRIMER_INTERNAL_2_SEQUENCE=AAGTTGTAAAGCCCAGTACACTTC PRIMER_LEFT_2=149,20 PRIMER_RIGHT_2=231,20 PRIMER_INTERNAL_2=187,24 PRIMER_LEFT_2_TM=60.517 PRIMER_RIGHT_2_TM=59.988 PRIMER_INTERNAL_2_TM=58.048 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.9000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=0.528743 PRIMER_LEFT_3_PENALTY=0.516683 PRIMER_RIGHT_3_PENALTY=0.012060 PRIMER_INTERNAL_3_PENALTY=5.952353 PRIMER_LEFT_3_SEQUENCE=CGATACCTTTGTCAGGGTGC PRIMER_RIGHT_3_SEQUENCE=TGATTTGCTTCAGTGCCAAG PRIMER_INTERNAL_3_SEQUENCE=AAGTTGTAAAGCCCAGTACACTTC PRIMER_LEFT_3=148,20 PRIMER_RIGHT_3=231,20 PRIMER_INTERNAL_3=187,24 PRIMER_LEFT_3_TM=60.517 PRIMER_RIGHT_3_TM=59.988 PRIMER_INTERNAL_3_TM=58.048 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=84 PRIMER_PAIR_4_PENALTY=0.530290 PRIMER_LEFT_4_PENALTY=0.516683 PRIMER_RIGHT_4_PENALTY=0.013607 PRIMER_INTERNAL_4_PENALTY=1.297410 PRIMER_LEFT_4_SEQUENCE=GATACCTTTGTCAGGGTGCG PRIMER_RIGHT_4_SEQUENCE=TCAGGGGATTGATTTGCTTC PRIMER_INTERNAL_4_SEQUENCE=CCCAGTACACTTCTCTTGGCA PRIMER_LEFT_4=149,20 PRIMER_RIGHT_4=240,20 PRIMER_INTERNAL_4=198,21 PRIMER_LEFT_4_TM=60.517 PRIMER_RIGHT_4_TM=60.014 PRIMER_INTERNAL_4_TM=60.297 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=9.9000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=92 = SEQUENCE_ID=MH684 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCAGGTGGACCAGTAGCGTTCTGTGGCCTGGGGGCGGAGGCCTCTAAGACACGGGTTGTGGCTATAAGATGAATGACAGCCATAAGGCCCACTCTGACATCTCTCGGGGCCGATGCACTATGTATCACTTAGCAAGATGCTGTAACCTGGTCTGGGGATTTCCAACCCTGGTCTGGGGAATTACTGGGTCTCCATGACAACAGGACCTAAAAAACCTGGTTGCCCTAGAGACAAGGCCTCCTCAGCACATATGCAACCTTCTTGAACCTTAAAACCAACCTTACTCTTACA SEQUENCE_INCLUDED_REGION=35,292 P3_COMMENT=CL is GTGGACCAGTAGCGTTCTGT P3_COMMENT=CR is AGGTCCTGTTGTCATGGAGA P3_COMMENT=CM is GGGCGGAGGCCTCTAAGA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.218298 PRIMER_LEFT_0_PENALTY=0.051910 PRIMER_RIGHT_0_PENALTY=0.166387 PRIMER_INTERNAL_0_PENALTY=0.934117 PRIMER_LEFT_0_SEQUENCE=ACCCTGGTCTGGGGAATTAC PRIMER_RIGHT_0_SEQUENCE=AGGAGGCCTTGTCTCTAGGG PRIMER_INTERNAL_0_SEQUENCE=GGGTCTCCATGACAACAGGA PRIMER_LEFT_0=200,20 PRIMER_RIGHT_0=277,20 PRIMER_INTERNAL_0=221,20 PRIMER_LEFT_0_TM=60.052 PRIMER_RIGHT_0_TM=59.834 PRIMER_INTERNAL_0_TM=60.934 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=5.6000 PRIMER_RIGHT_0_END_STABILITY=8.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 PRIMER_PAIR_1_PENALTY=0.228966 PRIMER_LEFT_1_PENALTY=0.051910 PRIMER_RIGHT_1_PENALTY=0.177056 PRIMER_INTERNAL_1_PENALTY=0.246438 PRIMER_LEFT_1_SEQUENCE=GGTCTGGGGAATTACTGGGT PRIMER_RIGHT_1_SEQUENCE=TTGCATATGTGCTGAGGAGG PRIMER_INTERNAL_1_SEQUENCE=TGGTTGCCCTAGAGACAAGG PRIMER_LEFT_1=205,20 PRIMER_RIGHT_1=291,20 PRIMER_INTERNAL_1=252,20 PRIMER_LEFT_1_TM=60.052 PRIMER_RIGHT_1_TM=59.823 PRIMER_INTERNAL_1_TM=60.246 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=2.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=87 PRIMER_PAIR_2_PENALTY=0.228966 PRIMER_LEFT_2_PENALTY=0.051910 PRIMER_RIGHT_2_PENALTY=0.177056 PRIMER_INTERNAL_2_PENALTY=0.246438 PRIMER_LEFT_2_SEQUENCE=ACCCTGGTCTGGGGAATTAC PRIMER_RIGHT_2_SEQUENCE=TTGCATATGTGCTGAGGAGG PRIMER_INTERNAL_2_SEQUENCE=TGGTTGCCCTAGAGACAAGG PRIMER_LEFT_2=200,20 PRIMER_RIGHT_2=291,20 PRIMER_INTERNAL_2=252,20 PRIMER_LEFT_2_TM=60.052 PRIMER_RIGHT_2_TM=59.823 PRIMER_INTERNAL_2_TM=60.246 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=5.6000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=92 PRIMER_PAIR_3_PENALTY=0.253140 PRIMER_LEFT_3_PENALTY=0.201230 PRIMER_RIGHT_3_PENALTY=0.051910 PRIMER_INTERNAL_3_PENALTY=0.010545 PRIMER_LEFT_3_SEQUENCE=GGCCCACTCTGACATCTCTC PRIMER_RIGHT_3_SEQUENCE=GTAATTCCCCAGACCAGGGT PRIMER_INTERNAL_3_SEQUENCE=ATGCTGTAACCTGGTCTGGG PRIMER_LEFT_3=121,20 PRIMER_RIGHT_3=219,20 PRIMER_INTERNAL_3=172,20 PRIMER_LEFT_3_TM=59.799 PRIMER_RIGHT_3_TM=60.052 PRIMER_INTERNAL_3_TM=59.989 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=9.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=0.256983 PRIMER_LEFT_4_PENALTY=0.010545 PRIMER_RIGHT_4_PENALTY=0.246438 PRIMER_INTERNAL_4_PENALTY=0.051910 PRIMER_LEFT_4_SEQUENCE=ATGCTGTAACCTGGTCTGGG PRIMER_RIGHT_4_SEQUENCE=TTGTCTCTAGGGCAACCAGG PRIMER_INTERNAL_4_SEQUENCE=GGTCTGGGGAATTACTGGGT PRIMER_LEFT_4=172,20 PRIMER_RIGHT_4=269,20 PRIMER_INTERNAL_4=205,20 PRIMER_LEFT_4_TM=59.989 PRIMER_RIGHT_4_TM=60.246 PRIMER_INTERNAL_4_TM=60.052 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH688 SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAAAGATTTTTAAAGTGTTTGTTTGGAATGTGTCAAGAGTNNTTTTGATGTGATATATCTAAGGTNACATGCAGAATCACTGAAATATTAAATGCCAAATGGTTTGCAAATAAACATGCAGATAATTATTTTNCTTCCCTAAATTTCAAGTTGGCACTAGCCAGCACTCTTGCCAAATGTTCCATCTATTTTCTTCCATTCTCAGCATCTTGCATGCTACCGTCCACGCTTCTCATAGTGATGCCTCATTACTGATAAGTTGTAATATCACTTGGTTGCCTATGACATTGAATCCAAG SEQUENCE_INCLUDED_REGION=35,300 P3_COMMENT=CL is TTAAATGCCAAATGGTTTGC P3_COMMENT=CR is TGTCATAGGCAACCAAGTGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.357620 PRIMER_LEFT_0_PENALTY=0.080808 PRIMER_RIGHT_0_PENALTY=0.276812 PRIMER_INTERNAL_0_PENALTY=0.503684 PRIMER_LEFT_0_SEQUENCE=AGTTGGCACTAGCCAGCACT PRIMER_RIGHT_0_SEQUENCE=ATGAGAAGCGTGGACGGTAG PRIMER_INTERNAL_0_SEQUENCE=TCCATTCTCAGCATCTTGCA PRIMER_LEFT_0=185,20 PRIMER_RIGHT_0=272,20 PRIMER_INTERNAL_0=231,20 PRIMER_LEFT_0_TM=60.081 PRIMER_RIGHT_0_TM=60.277 PRIMER_INTERNAL_0_TM=60.504 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 PRIMER_PAIR_1_PENALTY=0.357620 PRIMER_LEFT_1_PENALTY=0.080808 PRIMER_RIGHT_1_PENALTY=0.276812 PRIMER_INTERNAL_1_PENALTY=0.296338 PRIMER_LEFT_1_SEQUENCE=AGTTGGCACTAGCCAGCACT PRIMER_RIGHT_1_SEQUENCE=CTATGAGAAGCGTGGACGGT PRIMER_INTERNAL_1_SEQUENCE=TTCTCAGCATCTTGCATGCT PRIMER_LEFT_1=185,20 PRIMER_RIGHT_1=274,20 PRIMER_INTERNAL_1=235,20 PRIMER_LEFT_1_TM=60.081 PRIMER_RIGHT_1_TM=60.277 PRIMER_INTERNAL_1_TM=59.704 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=9.3000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=90 PRIMER_PAIR_2_PENALTY=0.357620 PRIMER_LEFT_2_PENALTY=0.080808 PRIMER_RIGHT_2_PENALTY=0.276812 PRIMER_INTERNAL_2_PENALTY=0.296338 PRIMER_LEFT_2_SEQUENCE=AGTTGGCACTAGCCAGCACT PRIMER_RIGHT_2_SEQUENCE=ACTATGAGAAGCGTGGACGG PRIMER_INTERNAL_2_SEQUENCE=TTCTCAGCATCTTGCATGCT PRIMER_LEFT_2=185,20 PRIMER_RIGHT_2=275,20 PRIMER_INTERNAL_2=235,20 PRIMER_LEFT_2_TM=60.081 PRIMER_RIGHT_2_TM=60.277 PRIMER_INTERNAL_2_TM=59.704 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=9.6000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=91 PRIMER_PAIR_3_PENALTY=0.372827 PRIMER_LEFT_3_PENALTY=0.064105 PRIMER_RIGHT_3_PENALTY=0.308722 PRIMER_INTERNAL_3_PENALTY=0.536418 PRIMER_LEFT_3_SEQUENCE=TGCCAAATGGTTTGCAAATA PRIMER_RIGHT_3_SEQUENCE=TGGAACATTTGGCAAGAGTG PRIMER_INTERNAL_3_SEQUENCE=TTTCAAGTTGGCACTAGCCA PRIMER_LEFT_3=129,20 PRIMER_RIGHT_3=220,20 PRIMER_INTERNAL_3=180,20 PRIMER_LEFT_3_TM=59.936 PRIMER_RIGHT_3_TM=59.691 PRIMER_INTERNAL_3_TM=59.464 PRIMER_LEFT_3_GC_PERCENT=35.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=6.2000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=9.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=92 PRIMER_PAIR_4_PENALTY=0.372827 PRIMER_LEFT_4_PENALTY=0.064105 PRIMER_RIGHT_4_PENALTY=0.308722 PRIMER_INTERNAL_4_PENALTY=0.536418 PRIMER_LEFT_4_SEQUENCE=TAAATGCCAAATGGTTTGCA PRIMER_RIGHT_4_SEQUENCE=TGGAACATTTGGCAAGAGTG PRIMER_INTERNAL_4_SEQUENCE=TTTCAAGTTGGCACTAGCCA PRIMER_LEFT_4=125,20 PRIMER_RIGHT_4=220,20 PRIMER_INTERNAL_4=180,20 PRIMER_LEFT_4_TM=59.936 PRIMER_RIGHT_4_TM=59.691 PRIMER_INTERNAL_4_TM=59.464 PRIMER_LEFT_4_GC_PERCENT=35.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=8.8000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=9.00 PRIMER_PAIR_4_COMPL_END=5.00 PRIMER_PAIR_4_PRODUCT_SIZE=96 = SEQUENCE_ID=MH691 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCCATGCCCCACAAAAACATACTTTTTGTGCTCCTGTTTATTAACTGGTACTTACTAAAAGCCAAATACTCCTAGAACTTTGTAGTATTATCCCTTTTAATTCCCACATCAACTAAGGTAGATGCTGTCTGTTATTGACTAAATTGTGTCTTCCCACAAAATTCAAATGTTGTAGCCCTAACTCTCAATGTGATGGTATTTAGAGACGGGGCTTTTGGGAGGTCATTAGGATTACATGAGGTCGTGGG SEQUENCE_INCLUDED_REGION=34,118 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.127760 PRIMER_LEFT_0_PENALTY=4.387445 PRIMER_RIGHT_0_PENALTY=1.740315 PRIMER_INTERNAL_0_PENALTY=6.319798 PRIMER_LEFT_0_SEQUENCE=TTTGTGCTCCTGTTTATTAACTGG PRIMER_RIGHT_0_SEQUENCE=TGATGTGGGAATTAAAAGGGA PRIMER_INTERNAL_0_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG PRIMER_LEFT_0=60,24 PRIMER_RIGHT_0=146,21 PRIMER_INTERNAL_0=92,26 PRIMER_LEFT_0_TM=59.613 PRIMER_RIGHT_0_TM=59.260 PRIMER_INTERNAL_0_TM=59.680 PRIMER_LEFT_0_GC_PERCENT=37.500 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_INTERNAL_0_GC_PERCENT=34.615 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=8.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=87 PRIMER_PAIR_1_PENALTY=6.357965 PRIMER_LEFT_1_PENALTY=4.617650 PRIMER_RIGHT_1_PENALTY=1.740315 PRIMER_INTERNAL_1_PENALTY=6.319798 PRIMER_LEFT_1_SEQUENCE=TTGTGCTCCTGTTTATTAACTGG PRIMER_RIGHT_1_SEQUENCE=TGATGTGGGAATTAAAAGGGA PRIMER_INTERNAL_1_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG PRIMER_LEFT_1=61,23 PRIMER_RIGHT_1=146,21 PRIMER_INTERNAL_1=92,26 PRIMER_LEFT_1_TM=58.382 PRIMER_RIGHT_1_TM=59.260 PRIMER_INTERNAL_1_TM=59.680 PRIMER_LEFT_1_GC_PERCENT=39.130 PRIMER_RIGHT_1_GC_PERCENT=38.095 PRIMER_INTERNAL_1_GC_PERCENT=34.615 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=8.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=86 PRIMER_PAIR_2_PENALTY=6.578207 PRIMER_LEFT_2_PENALTY=4.837892 PRIMER_RIGHT_2_PENALTY=1.740315 PRIMER_INTERNAL_2_PENALTY=6.319798 PRIMER_LEFT_2_SEQUENCE=TTGTGCTCCTGTTTATTAACTGGT PRIMER_RIGHT_2_SEQUENCE=TGATGTGGGAATTAAAAGGGA PRIMER_INTERNAL_2_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG PRIMER_LEFT_2=61,24 PRIMER_RIGHT_2=146,21 PRIMER_INTERNAL_2=92,26 PRIMER_LEFT_2_TM=59.162 PRIMER_RIGHT_2_TM=59.260 PRIMER_INTERNAL_2_TM=59.680 PRIMER_LEFT_2_GC_PERCENT=37.500 PRIMER_RIGHT_2_GC_PERCENT=38.095 PRIMER_INTERNAL_2_GC_PERCENT=34.615 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=86 PRIMER_PAIR_3_PENALTY=6.593719 PRIMER_LEFT_3_PENALTY=4.387445 PRIMER_RIGHT_3_PENALTY=2.206274 PRIMER_INTERNAL_3_PENALTY=6.319798 PRIMER_LEFT_3_SEQUENCE=TTTGTGCTCCTGTTTATTAACTGG PRIMER_RIGHT_3_SEQUENCE=TTGATGTGGGAATTAAAAGGG PRIMER_INTERNAL_3_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG PRIMER_LEFT_3=60,24 PRIMER_RIGHT_3=147,21 PRIMER_INTERNAL_3=92,26 PRIMER_LEFT_3_TM=59.613 PRIMER_RIGHT_3_TM=58.794 PRIMER_INTERNAL_3_TM=59.680 PRIMER_LEFT_3_GC_PERCENT=37.500 PRIMER_RIGHT_3_GC_PERCENT=38.095 PRIMER_INTERNAL_3_GC_PERCENT=34.615 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=8.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=88 PRIMER_PAIR_4_PENALTY=6.716551 PRIMER_LEFT_4_PENALTY=4.976237 PRIMER_RIGHT_4_PENALTY=1.740315 PRIMER_INTERNAL_4_PENALTY=6.319798 PRIMER_LEFT_4_SEQUENCE=TGTGCTCCTGTTTATTAACTGG PRIMER_RIGHT_4_SEQUENCE=TGATGTGGGAATTAAAAGGGA PRIMER_INTERNAL_4_SEQUENCE=AAAAGCCAAATACTCCTAGAACTTTG PRIMER_LEFT_4=62,22 PRIMER_RIGHT_4=146,21 PRIMER_INTERNAL_4=92,26 PRIMER_LEFT_4_TM=57.024 PRIMER_RIGHT_4_TM=59.260 PRIMER_INTERNAL_4_TM=59.680 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_INTERNAL_4_GC_PERCENT=34.615 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=8.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=85 = SEQUENCE_ID=MH692 SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCAAAAGAGGNNTAAAGAAACTAAAAGCAACAAAACCAAACTTAAAAAAAAAAAGGCAAAGGTTCTGAACTGCAAAGGAAATTGTCATACCATTAACTCTGCTGAATGAAAGAGGAATACAGCCTCCTCCGGTTCTTACAGTTGTGTATAAAATAAGGTAGCAGATTAGAAATAGAGAAGATATGCTAAAGATGAGATGTGACTGATGACAGCAGACAGCAAAGGAAGGAAGTATAATTGCTAACATGAAATAAAAGTGCTACAG SEQUENCE_INCLUDED_REGION=36,267 P3_COMMENT=CL is GGTTCTGAACTGCAAAGGAA P3_COMMENT=CR is TGTAGCACTTTTATTTCATGTTAGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.618156 PRIMER_LEFT_0_PENALTY=0.522443 PRIMER_RIGHT_0_PENALTY=0.095713 PRIMER_INTERNAL_0_PENALTY=3.799488 PRIMER_LEFT_0_SEQUENCE=AGGCAAAGGTTCTGAACTGC PRIMER_RIGHT_0_SEQUENCE=AGAACCGGAGGAGGCTGTAT PRIMER_INTERNAL_0_SEQUENCE=AACTCTGCTGAATGAAAGAGGA PRIMER_LEFT_0=91,20 PRIMER_RIGHT_0=173,20 PRIMER_INTERNAL_0=132,22 PRIMER_LEFT_0_TM=59.478 PRIMER_RIGHT_0_TM=60.096 PRIMER_INTERNAL_0_TM=58.201 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=5.6000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=83 PRIMER_PAIR_1_PENALTY=0.986123 PRIMER_LEFT_1_PENALTY=0.522443 PRIMER_RIGHT_1_PENALTY=0.463680 PRIMER_INTERNAL_1_PENALTY=4.206859 PRIMER_LEFT_1_SEQUENCE=AGGCAAAGGTTCTGAACTGC PRIMER_RIGHT_1_SEQUENCE=AACCGGAGGAGGCTGTATTC PRIMER_INTERNAL_1_SEQUENCE=CCATTAACTCTGCTGAATGAAAGA PRIMER_LEFT_1=91,20 PRIMER_RIGHT_1=171,20 PRIMER_INTERNAL_1=127,24 PRIMER_LEFT_1_TM=59.478 PRIMER_RIGHT_1_TM=60.464 PRIMER_INTERNAL_1_TM=59.793 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=5.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=81 PRIMER_PAIR_2_PENALTY=0.986123 PRIMER_LEFT_2_PENALTY=0.522443 PRIMER_RIGHT_2_PENALTY=0.463680 PRIMER_INTERNAL_2_PENALTY=4.206859 PRIMER_LEFT_2_SEQUENCE=AGGCAAAGGTTCTGAACTGC PRIMER_RIGHT_2_SEQUENCE=GAACCGGAGGAGGCTGTATT PRIMER_INTERNAL_2_SEQUENCE=CCATTAACTCTGCTGAATGAAAGA PRIMER_LEFT_2=91,20 PRIMER_RIGHT_2=172,20 PRIMER_INTERNAL_2=127,24 PRIMER_LEFT_2_TM=59.478 PRIMER_RIGHT_2_TM=60.464 PRIMER_INTERNAL_2_TM=59.793 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=5.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=82 PRIMER_PAIR_3_PENALTY=1.045650 PRIMER_LEFT_3_PENALTY=0.949937 PRIMER_RIGHT_3_PENALTY=0.095713 PRIMER_INTERNAL_3_PENALTY=3.799488 PRIMER_LEFT_3_SEQUENCE=GCAAAGGTTCTGAACTGCAA PRIMER_RIGHT_3_SEQUENCE=AGAACCGGAGGAGGCTGTAT PRIMER_INTERNAL_3_SEQUENCE=AACTCTGCTGAATGAAAGAGGA PRIMER_LEFT_3=93,20 PRIMER_RIGHT_3=173,20 PRIMER_INTERNAL_3=132,22 PRIMER_LEFT_3_TM=59.050 PRIMER_RIGHT_3_TM=60.096 PRIMER_INTERNAL_3_TM=58.201 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.8000 PRIMER_RIGHT_3_END_STABILITY=5.6000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=81 PRIMER_PAIR_4_PENALTY=1.413617 PRIMER_LEFT_4_PENALTY=0.949937 PRIMER_RIGHT_4_PENALTY=0.463680 PRIMER_INTERNAL_4_PENALTY=4.206859 PRIMER_LEFT_4_SEQUENCE=GCAAAGGTTCTGAACTGCAA PRIMER_RIGHT_4_SEQUENCE=AACCGGAGGAGGCTGTATTC PRIMER_INTERNAL_4_SEQUENCE=CCATTAACTCTGCTGAATGAAAGA PRIMER_LEFT_4=93,20 PRIMER_RIGHT_4=171,20 PRIMER_INTERNAL_4=127,24 PRIMER_LEFT_4_TM=59.050 PRIMER_RIGHT_4_TM=60.464 PRIMER_INTERNAL_4_TM=59.793 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=37.500 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.8000 PRIMER_RIGHT_4_END_STABILITY=5.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH693 SEQUENCE_TEMPLATE=GTGTGGGGGCTGGGATGAGAGGTCTACTTGTGGTACTGGAGGTTTCACTGGCTTGTGCTAGAACTAGNAAAGNAGAAAGAGACAGNGATTGGCTAACGCATGGCAGTAGTGGGCCCCAAGGCCTGAGTAATAAGAAAAAATCATTAGATAAATGTCTCATGACCAAAACAAAGTTCAAACANTAGGTGCAGCACANNNGGGTTTTCTCTGGTCATAGAATCTCTTAAAAGGGAATCATGACAGATTTTCTTGGCTTTAAGTCCTTTATTTAGTTCCCTCTACCAAGTGTATCTTTTAAAGCCTTGTTAAGA SEQUENCE_INCLUDED_REGION=0,311 P3_COMMENT=CL is GGCTGGGATGAGAGGTCTAC P3_COMMENT=CR is TAACAAGGCTTTAAAAGATACACTT P3_COMMENT=CM is TGTGGTACTGGAGGTTTCACT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.045873 PRIMER_LEFT_0_PENALTY=0.085951 PRIMER_RIGHT_0_PENALTY=0.959922 PRIMER_INTERNAL_0_PENALTY=0.299723 PRIMER_LEFT_0_SEQUENCE=AGGTTTCACTGGCTTGTGCT PRIMER_RIGHT_0_SEQUENCE=TTATTACTCAGGCCTTGGGG PRIMER_INTERNAL_0_SEQUENCE=GGCTAACGCATGGCAGTAGT PRIMER_LEFT_0=39,20 PRIMER_RIGHT_0=132,20 PRIMER_INTERNAL_0=90,20 PRIMER_LEFT_0_TM=59.914 PRIMER_RIGHT_0_TM=59.040 PRIMER_INTERNAL_0_TM=60.300 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=11.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=94 PRIMER_PAIR_1_PENALTY=1.119235 PRIMER_LEFT_1_PENALTY=0.085951 PRIMER_RIGHT_1_PENALTY=1.033284 PRIMER_INTERNAL_1_PENALTY=0.299723 PRIMER_LEFT_1_SEQUENCE=AGGTTTCACTGGCTTGTGCT PRIMER_RIGHT_1_SEQUENCE=CTTATTACTCAGGCCTTGGGG PRIMER_INTERNAL_1_SEQUENCE=GGCTAACGCATGGCAGTAGT PRIMER_LEFT_1=39,20 PRIMER_RIGHT_1=133,21 PRIMER_INTERNAL_1=90,20 PRIMER_LEFT_1_TM=59.914 PRIMER_RIGHT_1_TM=59.967 PRIMER_INTERNAL_1_TM=60.300 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=52.381 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=11.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=95 PRIMER_PAIR_2_PENALTY=1.406905 PRIMER_LEFT_2_PENALTY=0.085951 PRIMER_RIGHT_2_PENALTY=1.320954 PRIMER_INTERNAL_2_PENALTY=0.299723 PRIMER_LEFT_2_SEQUENCE=AGGTTTCACTGGCTTGTGCT PRIMER_RIGHT_2_SEQUENCE=TATTACTCAGGCCTTGGGGC PRIMER_INTERNAL_2_SEQUENCE=GGCTAACGCATGGCAGTAGT PRIMER_LEFT_2=39,20 PRIMER_RIGHT_2=131,20 PRIMER_INTERNAL_2=90,20 PRIMER_LEFT_2_TM=59.914 PRIMER_RIGHT_2_TM=61.321 PRIMER_INTERNAL_2_TM=60.300 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=12.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=93 PRIMER_PAIR_3_PENALTY=1.649603 PRIMER_LEFT_3_PENALTY=0.689681 PRIMER_RIGHT_3_PENALTY=0.959922 PRIMER_INTERNAL_3_PENALTY=0.299723 PRIMER_LEFT_3_SEQUENCE=GGAGGTTTCACTGGCTTGTG PRIMER_RIGHT_3_SEQUENCE=TTATTACTCAGGCCTTGGGG PRIMER_INTERNAL_3_SEQUENCE=GGCTAACGCATGGCAGTAGT PRIMER_LEFT_3=37,20 PRIMER_RIGHT_3=132,20 PRIMER_INTERNAL_3=90,20 PRIMER_LEFT_3_TM=60.690 PRIMER_RIGHT_3_TM=59.040 PRIMER_INTERNAL_3_TM=60.300 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=11.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=96 PRIMER_PAIR_4_PENALTY=1.649603 PRIMER_LEFT_4_PENALTY=0.689681 PRIMER_RIGHT_4_PENALTY=0.959922 PRIMER_INTERNAL_4_PENALTY=0.299723 PRIMER_LEFT_4_SEQUENCE=TGGAGGTTTCACTGGCTTGT PRIMER_RIGHT_4_SEQUENCE=TTATTACTCAGGCCTTGGGG PRIMER_INTERNAL_4_SEQUENCE=GGCTAACGCATGGCAGTAGT PRIMER_LEFT_4=36,20 PRIMER_RIGHT_4=132,20 PRIMER_INTERNAL_4=90,20 PRIMER_LEFT_4_TM=60.690 PRIMER_RIGHT_4_TM=59.040 PRIMER_INTERNAL_4_TM=60.300 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=11.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=97 = SEQUENCE_ID=MH694 SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTAAAAAATGTTTATGTTGATATAACTCAAGCCCAGGCACCATTTCTCCTCATTTTCCTTTCAATGTGCTTTTCAAAAATTATGGCATATGTCTGCCTGAAAGTCGGAAACATTTTTGACAATAACTTGGAACTTTCTCTAACCTAATACACTAGATTTAAAGTGAATTATACCGCCATAGGTTGACATGGAGCCATTTTAATAGCAGGTAGTTGACCTGAGAACTCCAAAGCAATTGCTTACATATTGAATTATCTTATA SEQUENCE_INCLUDED_REGION=36,215 P3_COMMENT=CL is TGTTTATGTTGATATAACTCAAGCC P3_COMMENT=CR is ACCTGCTATTAAAATGGCTCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.448041 PRIMER_LEFT_0_PENALTY=0.072951 PRIMER_RIGHT_0_PENALTY=0.375090 PRIMER_INTERNAL_0_PENALTY=2.263560 PRIMER_LEFT_0_SEQUENCE=CAGGCACCATTTCTCCTCAT PRIMER_RIGHT_0_SEQUENCE=TTTCCGACTTTCAGGCAGAC PRIMER_INTERNAL_0_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA PRIMER_LEFT_0=69,20 PRIMER_RIGHT_0=145,20 PRIMER_INTERNAL_0=91,22 PRIMER_LEFT_0_TM=60.073 PRIMER_RIGHT_0_TM=60.375 PRIMER_INTERNAL_0_TM=59.736 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=31.818 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=0.448041 PRIMER_LEFT_1_PENALTY=0.072951 PRIMER_RIGHT_1_PENALTY=0.375090 PRIMER_INTERNAL_1_PENALTY=2.263560 PRIMER_LEFT_1_SEQUENCE=CAGGCACCATTTCTCCTCAT PRIMER_RIGHT_1_SEQUENCE=GTTTCCGACTTTCAGGCAGA PRIMER_INTERNAL_1_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA PRIMER_LEFT_1=69,20 PRIMER_RIGHT_1=146,20 PRIMER_INTERNAL_1=91,22 PRIMER_LEFT_1_TM=60.073 PRIMER_RIGHT_1_TM=60.375 PRIMER_INTERNAL_1_TM=59.736 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=31.818 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=0.708984 PRIMER_LEFT_2_PENALTY=0.072951 PRIMER_RIGHT_2_PENALTY=0.636033 PRIMER_INTERNAL_2_PENALTY=2.263560 PRIMER_LEFT_2_SEQUENCE=CAGGCACCATTTCTCCTCAT PRIMER_RIGHT_2_SEQUENCE=ATGTTTCCGACTTTCAGGCA PRIMER_INTERNAL_2_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA PRIMER_LEFT_2=69,20 PRIMER_RIGHT_2=148,20 PRIMER_INTERNAL_2=91,22 PRIMER_LEFT_2_TM=60.073 PRIMER_RIGHT_2_TM=60.636 PRIMER_INTERNAL_2_TM=59.736 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=31.818 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=0.874143 PRIMER_LEFT_3_PENALTY=0.072951 PRIMER_RIGHT_3_PENALTY=0.801192 PRIMER_INTERNAL_3_PENALTY=2.263560 PRIMER_LEFT_3_SEQUENCE=CAGGCACCATTTCTCCTCAT PRIMER_RIGHT_3_SEQUENCE=TCCGACTTTCAGGCAGACAT PRIMER_INTERNAL_3_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA PRIMER_LEFT_3=69,20 PRIMER_RIGHT_3=143,20 PRIMER_INTERNAL_3=91,22 PRIMER_LEFT_3_TM=60.073 PRIMER_RIGHT_3_TM=60.801 PRIMER_INTERNAL_3_TM=59.736 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=31.818 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=6.3000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=0.898749 PRIMER_LEFT_4_PENALTY=0.072951 PRIMER_RIGHT_4_PENALTY=0.825798 PRIMER_INTERNAL_4_PENALTY=2.263560 PRIMER_LEFT_4_SEQUENCE=CAGGCACCATTTCTCCTCAT PRIMER_RIGHT_4_SEQUENCE=AATGTTTCCGACTTTCAGGC PRIMER_INTERNAL_4_SEQUENCE=TCCTTTCAATGTGCTTTTCAAA PRIMER_LEFT_4=69,20 PRIMER_RIGHT_4=149,20 PRIMER_INTERNAL_4=91,22 PRIMER_LEFT_4_TM=60.073 PRIMER_RIGHT_4_TM=59.174 PRIMER_INTERNAL_4_TM=59.736 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=31.818 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=81 = SEQUENCE_ID=MH698 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCTGGATGGCAGACACCTGAGGAGCCAGGTGCCTGAAATTTCCTCCACCTGCTTGAGGAGCGCCAAAGCCCAGGTGCTGGCTAGACAACCCCTTCTGGCTGCCTAAGCAGGTGGCAGAGGAAGGAAAAAAGGTCAGAGGCAGAGTNTTGAACCCTGCCTCCCAGGTGGGTGGAAGATGCCTGTCGCCAAACTAGGGCTCAGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT SEQUENCE_INCLUDED_REGION=36,198 P3_COMMENT=CL is GGCAGACACCTGAGGAGC P3_COMMENT=CR is CAGGCATCTTCCACCCAC P3_COMMENT=CM is AGGTGCCTGAAATTTCCTCC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.509144 PRIMER_LEFT_0_PENALTY=0.329550 PRIMER_RIGHT_0_PENALTY=0.179594 PRIMER_INTERNAL_0_PENALTY=0.246438 PRIMER_LEFT_0_SEQUENCE=AATTTCCTCCACCTGCTTGA PRIMER_RIGHT_0_SEQUENCE=CCTTTTTTCCTTCCTCTGCC PRIMER_INTERNAL_0_SEQUENCE=TAGACAACCCCTTCTGGCTG PRIMER_LEFT_0=70,20 PRIMER_RIGHT_0=165,20 PRIMER_INTERNAL_0=115,20 PRIMER_LEFT_0_TM=59.670 PRIMER_RIGHT_0_TM=60.180 PRIMER_INTERNAL_0_TM=60.246 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=2.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=96 PRIMER_PAIR_1_PENALTY=0.509144 PRIMER_LEFT_1_PENALTY=0.329550 PRIMER_RIGHT_1_PENALTY=0.179594 PRIMER_INTERNAL_1_PENALTY=0.246438 PRIMER_LEFT_1_SEQUENCE=TGAAATTTCCTCCACCTGCT PRIMER_RIGHT_1_SEQUENCE=CCTTTTTTCCTTCCTCTGCC PRIMER_INTERNAL_1_SEQUENCE=TAGACAACCCCTTCTGGCTG PRIMER_LEFT_1=67,20 PRIMER_RIGHT_1=165,20 PRIMER_INTERNAL_1=115,20 PRIMER_LEFT_1_TM=59.670 PRIMER_RIGHT_1_TM=60.180 PRIMER_INTERNAL_1_TM=60.246 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=0.509144 PRIMER_LEFT_2_PENALTY=0.329550 PRIMER_RIGHT_2_PENALTY=0.179594 PRIMER_INTERNAL_2_PENALTY=0.246438 PRIMER_LEFT_2_SEQUENCE=CTGAAATTTCCTCCACCTGC PRIMER_RIGHT_2_SEQUENCE=CCTTTTTTCCTTCCTCTGCC PRIMER_INTERNAL_2_SEQUENCE=TAGACAACCCCTTCTGGCTG PRIMER_LEFT_2=66,20 PRIMER_RIGHT_2=165,20 PRIMER_INTERNAL_2=115,20 PRIMER_LEFT_2_TM=59.670 PRIMER_RIGHT_2_TM=60.180 PRIMER_INTERNAL_2_TM=60.246 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=0.575987 PRIMER_LEFT_3_PENALTY=0.329550 PRIMER_RIGHT_3_PENALTY=0.246438 PRIMER_INTERNAL_3_PENALTY=4.173858 PRIMER_LEFT_3_SEQUENCE=CAGGTGCCTGAAATTTCCTC PRIMER_RIGHT_3_SEQUENCE=CAGCCAGAAGGGGTTGTCTA PRIMER_INTERNAL_3_SEQUENCE=CTTGAGGAGCGCCAAAGC PRIMER_LEFT_3=59,20 PRIMER_RIGHT_3=134,20 PRIMER_INTERNAL_3=85,18 PRIMER_LEFT_3_TM=59.670 PRIMER_RIGHT_3_TM=60.246 PRIMER_INTERNAL_3_TM=62.174 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=61.111 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=5.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=0.689335 PRIMER_LEFT_4_PENALTY=0.442898 PRIMER_RIGHT_4_PENALTY=0.246438 PRIMER_INTERNAL_4_PENALTY=4.173858 PRIMER_LEFT_4_SEQUENCE=AGGTGCCTGAAATTTCCTCC PRIMER_RIGHT_4_SEQUENCE=CAGCCAGAAGGGGTTGTCTA PRIMER_INTERNAL_4_SEQUENCE=CTTGAGGAGCGCCAAAGC PRIMER_LEFT_4=60,20 PRIMER_RIGHT_4=134,20 PRIMER_INTERNAL_4=85,18 PRIMER_LEFT_4_TM=60.443 PRIMER_RIGHT_4_TM=60.246 PRIMER_INTERNAL_4_TM=62.174 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=5.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH701 SEQUENCE_TEMPLATE=NTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGTTTCATCATATGCGAAACATGGATAAAAACAACTACCTTGCCTTACCTACTTTACAGAGTNTCTCCCCACAATTCCAAGAATTAAATGAAGAAAATGTCTGTAAAAGTGTTTTGCATGCTATAATGTAAAGCATCATTGTGAGATCTGTGATGGTTATCCCAGGTCTTTATTCCAACATCCATTTTCTTATTAATTTTTATAACTTCCATTTTTATCAGTGGGCCTCTTATTCACTAGATTTATCTGATGAAAGCCATCTGGAATGCATATTTACCAGGTTA SEQUENCE_INCLUDED_REGION=35,156 P3_COMMENT=CL is TCATCATATGCGAAACATGG P3_COMMENT=CR is TCACAGATCTCACAATGATGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.524112 PRIMER_LEFT_0_PENALTY=1.094139 PRIMER_RIGHT_0_PENALTY=0.429973 PRIMER_INTERNAL_0_PENALTY=5.075685 PRIMER_LEFT_0_SEQUENCE=TCATCATATGCGAAACATGGA PRIMER_RIGHT_0_SEQUENCE=TTCTTGGAATTGTGGGGAGA PRIMER_INTERNAL_0_SEQUENCE=AAAAACAACTACCTTGCCTTACC PRIMER_LEFT_0=41,21 PRIMER_RIGHT_0=119,20 PRIMER_INTERNAL_0=63,23 PRIMER_LEFT_0_TM=59.906 PRIMER_RIGHT_0_TM=60.430 PRIMER_INTERNAL_0_TM=57.924 PRIMER_LEFT_0_GC_PERCENT=38.095 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=39.130 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.1000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=79 PRIMER_PAIR_1_PENALTY=1.873877 PRIMER_LEFT_1_PENALTY=1.094139 PRIMER_RIGHT_1_PENALTY=0.779738 PRIMER_INTERNAL_1_PENALTY=5.075685 PRIMER_LEFT_1_SEQUENCE=TCATCATATGCGAAACATGGA PRIMER_RIGHT_1_SEQUENCE=AATTCTTGGAATTGTGGGGA PRIMER_INTERNAL_1_SEQUENCE=AAAAACAACTACCTTGCCTTACC PRIMER_LEFT_1=41,21 PRIMER_RIGHT_1=121,20 PRIMER_INTERNAL_1=63,23 PRIMER_LEFT_1_TM=59.906 PRIMER_RIGHT_1_TM=59.220 PRIMER_INTERNAL_1_TM=57.924 PRIMER_LEFT_1_GC_PERCENT=38.095 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.1000 PRIMER_RIGHT_1_END_STABILITY=10.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=81 PRIMER_PAIR_2_PENALTY=2.038891 PRIMER_LEFT_2_PENALTY=1.608918 PRIMER_RIGHT_2_PENALTY=0.429973 PRIMER_INTERNAL_2_PENALTY=5.075685 PRIMER_LEFT_2_SEQUENCE=TTCATCATATGCGAAACATGG PRIMER_RIGHT_2_SEQUENCE=TTCTTGGAATTGTGGGGAGA PRIMER_INTERNAL_2_SEQUENCE=AAAAACAACTACCTTGCCTTACC PRIMER_LEFT_2=40,21 PRIMER_RIGHT_2=119,20 PRIMER_INTERNAL_2=63,23 PRIMER_LEFT_2_TM=59.391 PRIMER_RIGHT_2_TM=60.430 PRIMER_INTERNAL_2_TM=57.924 PRIMER_LEFT_2_GC_PERCENT=38.095 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=2.258256 PRIMER_LEFT_3_PENALTY=1.094139 PRIMER_RIGHT_3_PENALTY=1.164117 PRIMER_INTERNAL_3_PENALTY=5.075685 PRIMER_LEFT_3_SEQUENCE=TCATCATATGCGAAACATGGA PRIMER_RIGHT_3_SEQUENCE=ATTCTTGGAATTGTGGGGAG PRIMER_INTERNAL_3_SEQUENCE=AAAAACAACTACCTTGCCTTACC PRIMER_LEFT_3=41,21 PRIMER_RIGHT_3=120,20 PRIMER_INTERNAL_3=63,23 PRIMER_LEFT_3_TM=59.906 PRIMER_RIGHT_3_TM=58.836 PRIMER_INTERNAL_3_TM=57.924 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=2.266978 PRIMER_LEFT_4_PENALTY=1.094139 PRIMER_RIGHT_4_PENALTY=1.172839 PRIMER_INTERNAL_4_PENALTY=5.075685 PRIMER_LEFT_4_SEQUENCE=TCATCATATGCGAAACATGGA PRIMER_RIGHT_4_SEQUENCE=AATTCTTGGAATTGTGGGGAG PRIMER_INTERNAL_4_SEQUENCE=AAAAACAACTACCTTGCCTTACC PRIMER_LEFT_4=41,21 PRIMER_RIGHT_4=121,21 PRIMER_INTERNAL_4=63,23 PRIMER_LEFT_4_TM=59.906 PRIMER_RIGHT_4_TM=60.173 PRIMER_INTERNAL_4_TM=57.924 PRIMER_LEFT_4_GC_PERCENT=38.095 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=81 = SEQUENCE_ID=MH704 SEQUENCE_TEMPLATE=TCGACTCTAGAGGATCCCCCTAACTCTTGTNTTTCTAAGCACTTAACAATGGATAATGNAAACATTCTAGGGAAGGGTACCNNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTTGCATTAATGAATCGGCCAACGCGCGGGGAG SEQUENCE_INCLUDED_REGION=19,55 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH707 SEQUENCE_TEMPLATE=TATATGGATTTTTATCTTGGGTNTAATTATTAGGTTATTACAAAAGTAATTATGTTTTTTGGCATTATTTTCAAATGGCAAAAACCGCAGTTACTTTTGCAACAGCCTAATATTTAATAGCCTTGCTTCTTTCTTGGAAACTGTTCTTGTTACTTTGAGTNTCTACCAGTNCCATCTTTTCTTGTTCACGATGTAATGTCTGTTTGGACAGATGTATCAAAGTACCTTAATCTAAG SEQUENCE_INCLUDED_REGION=0,236 P3_COMMENT=CL is TTTGGCATTATTTTCAAATGG P3_COMMENT=CR is TGTCCAAACAGACATTACATCG P3_COMMENT=CM is AAAAACCGCAGTTACTTTTGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.634264 PRIMER_LEFT_0_PENALTY=1.203207 PRIMER_RIGHT_0_PENALTY=0.431057 PRIMER_INTERNAL_0_PENALTY=0.088865 PRIMER_LEFT_0_SEQUENCE=GGCATTATTTTCAAATGGCAA PRIMER_RIGHT_0_SEQUENCE=TTCCAAGAAAGAAGCAAGGC PRIMER_INTERNAL_0_SEQUENCE=CCGCAGTTACTTTTGCAACA PRIMER_LEFT_0=60,21 PRIMER_RIGHT_0=138,20 PRIMER_INTERNAL_0=84,20 PRIMER_LEFT_0_TM=59.797 PRIMER_RIGHT_0_TM=59.569 PRIMER_INTERNAL_0_TM=59.911 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=79 PRIMER_PAIR_1_PENALTY=1.634264 PRIMER_LEFT_1_PENALTY=1.203207 PRIMER_RIGHT_1_PENALTY=0.431057 PRIMER_INTERNAL_1_PENALTY=0.088865 PRIMER_LEFT_1_SEQUENCE=TTGGCATTATTTTCAAATGGC PRIMER_RIGHT_1_SEQUENCE=TTCCAAGAAAGAAGCAAGGC PRIMER_INTERNAL_1_SEQUENCE=CCGCAGTTACTTTTGCAACA PRIMER_LEFT_1=58,21 PRIMER_RIGHT_1=138,20 PRIMER_INTERNAL_1=84,20 PRIMER_LEFT_1_TM=59.797 PRIMER_RIGHT_1_TM=59.569 PRIMER_INTERNAL_1_TM=59.911 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=81 PRIMER_PAIR_2_PENALTY=1.943817 PRIMER_LEFT_2_PENALTY=1.512759 PRIMER_RIGHT_2_PENALTY=0.431057 PRIMER_INTERNAL_2_PENALTY=0.088865 PRIMER_LEFT_2_SEQUENCE=GGCATTATTTTCAAATGGCA PRIMER_RIGHT_2_SEQUENCE=TTCCAAGAAAGAAGCAAGGC PRIMER_INTERNAL_2_SEQUENCE=CCGCAGTTACTTTTGCAACA PRIMER_LEFT_2=60,20 PRIMER_RIGHT_2=138,20 PRIMER_INTERNAL_2=84,20 PRIMER_LEFT_2_TM=58.487 PRIMER_RIGHT_2_TM=59.569 PRIMER_INTERNAL_2_TM=59.911 PRIMER_LEFT_2_GC_PERCENT=35.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=10.0000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=79 PRIMER_PAIR_3_PENALTY=1.943817 PRIMER_LEFT_3_PENALTY=1.512759 PRIMER_RIGHT_3_PENALTY=0.431057 PRIMER_INTERNAL_3_PENALTY=0.088865 PRIMER_LEFT_3_SEQUENCE=TGGCATTATTTTCAAATGGC PRIMER_RIGHT_3_SEQUENCE=TTCCAAGAAAGAAGCAAGGC PRIMER_INTERNAL_3_SEQUENCE=CCGCAGTTACTTTTGCAACA PRIMER_LEFT_3=59,20 PRIMER_RIGHT_3=138,20 PRIMER_INTERNAL_3=84,20 PRIMER_LEFT_3_TM=58.487 PRIMER_RIGHT_3_TM=59.569 PRIMER_INTERNAL_3_TM=59.911 PRIMER_LEFT_3_GC_PERCENT=35.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=9.6000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=2.008682 PRIMER_LEFT_4_PENALTY=1.203207 PRIMER_RIGHT_4_PENALTY=0.805475 PRIMER_INTERNAL_4_PENALTY=0.088865 PRIMER_LEFT_4_SEQUENCE=GGCATTATTTTCAAATGGCAA PRIMER_RIGHT_4_SEQUENCE=TCCAAGAAAGAAGCAAGGCT PRIMER_INTERNAL_4_SEQUENCE=CCGCAGTTACTTTTGCAACA PRIMER_LEFT_4=60,21 PRIMER_RIGHT_4=137,20 PRIMER_INTERNAL_4=84,20 PRIMER_LEFT_4_TM=59.797 PRIMER_RIGHT_4_TM=59.195 PRIMER_INTERNAL_4_TM=59.911 PRIMER_LEFT_4_GC_PERCENT=33.333 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=78 = SEQUENCE_ID=MH714 SEQUENCE_TEMPLATE=CTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTGTAACCTGGACAAATGACTATTGATAAAGAATTTATTTTCTGTCACCTTTAAGGAGAGCAGAGGGTGGTACAGCCTTTGTATTATTTTTNCTTTTTTAAAAAAAGTTTGTTTGAAGCCTCCTTCATGAAAACTTCAGCTGTCAAATCTTGGCAAAAAATGTGTGTTCATCTATATGATAGTTCTTAGGAGAATCAATAG SEQUENCE_INCLUDED_REGION=35,201 P3_COMMENT=CL is TTCTGTCACCTTTAAGGAGAGC P3_COMMENT=CR is TGAACACACATTTTTTGCCA P3_COMMENT=CM is GAGGGTGGTACAGCCTTTGT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.532509 PRIMER_LEFT_0_PENALTY=0.520448 PRIMER_RIGHT_0_PENALTY=0.012060 PRIMER_INTERNAL_0_PENALTY=1.152564 PRIMER_LEFT_0_SEQUENCE=AGAGCAGAGGGTGGTACAGC PRIMER_RIGHT_0_SEQUENCE=TTGCCAAGATTTGACAGCTG PRIMER_INTERNAL_0_SEQUENCE=TGTTTGAAGCCTCCTTCATG PRIMER_LEFT_0=91,20 PRIMER_RIGHT_0=190,20 PRIMER_INTERNAL_0=144,20 PRIMER_LEFT_0_TM=59.480 PRIMER_RIGHT_0_TM=59.988 PRIMER_INTERNAL_0_TM=58.847 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=0.804296 PRIMER_LEFT_1_PENALTY=0.792236 PRIMER_RIGHT_1_PENALTY=0.012060 PRIMER_INTERNAL_1_PENALTY=1.152564 PRIMER_LEFT_1_SEQUENCE=AGAGGGTGGTACAGCCTTTG PRIMER_RIGHT_1_SEQUENCE=TTGCCAAGATTTGACAGCTG PRIMER_INTERNAL_1_SEQUENCE=TGTTTGAAGCCTCCTTCATG PRIMER_LEFT_1=96,20 PRIMER_RIGHT_1=190,20 PRIMER_INTERNAL_1=144,20 PRIMER_LEFT_1_TM=59.208 PRIMER_RIGHT_1_TM=59.988 PRIMER_INTERNAL_1_TM=58.847 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=95 PRIMER_PAIR_2_PENALTY=0.804296 PRIMER_LEFT_2_PENALTY=0.792236 PRIMER_RIGHT_2_PENALTY=0.012060 PRIMER_INTERNAL_2_PENALTY=1.152564 PRIMER_LEFT_2_SEQUENCE=CAGAGGGTGGTACAGCCTTT PRIMER_RIGHT_2_SEQUENCE=TTGCCAAGATTTGACAGCTG PRIMER_INTERNAL_2_SEQUENCE=TGTTTGAAGCCTCCTTCATG PRIMER_LEFT_2=95,20 PRIMER_RIGHT_2=190,20 PRIMER_INTERNAL_2=144,20 PRIMER_LEFT_2_TM=59.208 PRIMER_RIGHT_2_TM=59.988 PRIMER_INTERNAL_2_TM=58.847 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=0.946936 PRIMER_LEFT_3_PENALTY=0.934876 PRIMER_RIGHT_3_PENALTY=0.012060 PRIMER_INTERNAL_3_PENALTY=1.152564 PRIMER_LEFT_3_SEQUENCE=GAGGGTGGTACAGCCTTTGT PRIMER_RIGHT_3_SEQUENCE=TTGCCAAGATTTGACAGCTG PRIMER_INTERNAL_3_SEQUENCE=TGTTTGAAGCCTCCTTCATG PRIMER_LEFT_3=97,20 PRIMER_RIGHT_3=190,20 PRIMER_INTERNAL_3=144,20 PRIMER_LEFT_3_TM=59.065 PRIMER_RIGHT_3_TM=59.988 PRIMER_INTERNAL_3_TM=58.847 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=1.057493 PRIMER_LEFT_4_PENALTY=1.045432 PRIMER_RIGHT_4_PENALTY=0.012060 PRIMER_INTERNAL_4_PENALTY=1.152564 PRIMER_LEFT_4_SEQUENCE=AGAGGGTGGTACAGCCTTTGT PRIMER_RIGHT_4_SEQUENCE=TTGCCAAGATTTGACAGCTG PRIMER_INTERNAL_4_SEQUENCE=TGTTTGAAGCCTCCTTCATG PRIMER_LEFT_4=96,21 PRIMER_RIGHT_4=190,20 PRIMER_INTERNAL_4=144,20 PRIMER_LEFT_4_TM=60.045 PRIMER_RIGHT_4_TM=59.988 PRIMER_INTERNAL_4_TM=58.847 PRIMER_LEFT_4_GC_PERCENT=52.381 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=95 = SEQUENCE_ID=MH715 SEQUENCE_TEMPLATE=AGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTCTTCCTGAGAACAAAGGGCTCAGGCTATGATGTGGGGAGGAGGCCTGGACCTGTGTCCTATGCCTGGGNCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT SEQUENCE_INCLUDED_REGION=37,76 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH718 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTCTTTGGCTATCTCATTCTCACACCTCCCTCATCACCTTTTTCTTGGGCACGTGCATTTCTTTTTCTCCTAGGGTTGAGCACAGATCTAACTTGAATAATTAAAGGAAGCTTTGTGTAATGAAAGCCAAAGCAGAAAGGAACAAAAGACTTTTAGCATGTAATAGAAGGGAATAGTGAACATGGAGGCAGAGGGAGTCATTTTTGGGGTGTAAGAGTCGATTATCTTTCTTGTCCCCTGGGCCGTTCGTTAGGGTGGCTTTACTTTGTTAATCCTGCACTT SEQUENCE_INCLUDED_REGION=36,209 P3_COMMENT=CL is TGGCTATCTCATTCTCACACC P3_COMMENT=CR is AAAAATGACTCCCTCTGCCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.336494 PRIMER_LEFT_0_PENALTY=0.006945 PRIMER_RIGHT_0_PENALTY=0.329550 PRIMER_INTERNAL_0_PENALTY=4.331778 PRIMER_LEFT_0_SEQUENCE=AGCCAAAGCAGAAAGGAACA PRIMER_RIGHT_0_SEQUENCE=CAAAAATGACTCCCTCTGCC PRIMER_INTERNAL_0_SEQUENCE=AGAAGGGAATAGTGAACATGGA PRIMER_LEFT_0=162,20 PRIMER_RIGHT_0=243,20 PRIMER_INTERNAL_0=202,22 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=59.670 PRIMER_INTERNAL_0_TM=57.668 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=0.336494 PRIMER_LEFT_1_PENALTY=0.006945 PRIMER_RIGHT_1_PENALTY=0.329550 PRIMER_INTERNAL_1_PENALTY=4.331778 PRIMER_LEFT_1_SEQUENCE=AGCCAAAGCAGAAAGGAACA PRIMER_RIGHT_1_SEQUENCE=CCAAAAATGACTCCCTCTGC PRIMER_INTERNAL_1_SEQUENCE=GAAGGGAATAGTGAACATGGAG PRIMER_LEFT_1=162,20 PRIMER_RIGHT_1=244,20 PRIMER_INTERNAL_1=203,22 PRIMER_LEFT_1_TM=59.993 PRIMER_RIGHT_1_TM=59.670 PRIMER_INTERNAL_1_TM=57.668 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=0.602767 PRIMER_LEFT_2_PENALTY=0.006945 PRIMER_RIGHT_2_PENALTY=0.595822 PRIMER_INTERNAL_2_PENALTY=7.082413 PRIMER_LEFT_2_SEQUENCE=AGCCAAAGCAGAAAGGAACA PRIMER_RIGHT_2_SEQUENCE=AAATGACTCCCTCTGCCTCC PRIMER_INTERNAL_2_SEQUENCE=GCATGTAATAGAAGGGAATAGTGAA PRIMER_LEFT_2=162,20 PRIMER_RIGHT_2=240,20 PRIMER_INTERNAL_2=193,25 PRIMER_LEFT_2_TM=59.993 PRIMER_RIGHT_2_TM=60.596 PRIMER_INTERNAL_2_TM=57.918 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=36.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=79 PRIMER_PAIR_3_PENALTY=0.633316 PRIMER_LEFT_3_PENALTY=0.626371 PRIMER_RIGHT_3_PENALTY=0.006945 PRIMER_INTERNAL_3_PENALTY=1.701644 PRIMER_LEFT_3_SEQUENCE=GGCACGTGCATTTCTTTTTC PRIMER_RIGHT_3_SEQUENCE=TGTTCCTTTCTGCTTTGGCT PRIMER_INTERNAL_3_SEQUENCE=TCCTAGGGTTGAGCACAGATC PRIMER_LEFT_3=85,20 PRIMER_RIGHT_3=181,20 PRIMER_INTERNAL_3=105,21 PRIMER_LEFT_3_TM=60.626 PRIMER_RIGHT_3_TM=59.993 PRIMER_INTERNAL_3_TM=59.298 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=0.694720 PRIMER_LEFT_4_PENALTY=0.365170 PRIMER_RIGHT_4_PENALTY=0.329550 PRIMER_INTERNAL_4_PENALTY=4.331778 PRIMER_LEFT_4_SEQUENCE=GCCAAAGCAGAAAGGAACAA PRIMER_RIGHT_4_SEQUENCE=CAAAAATGACTCCCTCTGCC PRIMER_INTERNAL_4_SEQUENCE=AGAAGGGAATAGTGAACATGGA PRIMER_LEFT_4=163,20 PRIMER_RIGHT_4=243,20 PRIMER_INTERNAL_4=202,22 PRIMER_LEFT_4_TM=60.365 PRIMER_RIGHT_4_TM=59.670 PRIMER_INTERNAL_4_TM=57.668 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=81 = SEQUENCE_ID=MH719 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTAAGTNTCTTAAATCTTTGCTTCAAAGAAGTTCTTGCTCAGGAAGTTATTCATTCAGCAACCTAAAATTGTTTTTTGAGTACATATCAAGCACAGGGTTCTGAGCAATGTCTTAGGAAGACCATAAAGGTGAATAAATGAGTGTTTCTACCCTGAGGAATTTATCAAAGATGTTAAGTTATCTCCTTAGAGGTATAAGTCATATAGGCATATTCTATGTATACTAAAGGTGGTATGGCATAAGAGTACATATAGTCACCAACTTANGAATTGTTTGACTTGGNCGGGCGCAGTGGCTCACG SEQUENCE_INCLUDED_REGION=36,303 P3_COMMENT=CL is TTGCTTCAAAGAAGTTCTTGC P3_COMMENT=CR is TATGTACTCTTATGCCATACCACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.887466 PRIMER_LEFT_0_PENALTY=2.761640 PRIMER_RIGHT_0_PENALTY=0.125826 PRIMER_INTERNAL_0_PENALTY=4.392765 PRIMER_LEFT_0_SEQUENCE=TCAAAGAAGTTCTTGCTCAGGA PRIMER_RIGHT_0_SEQUENCE=ATTGCTCAGAACCCTGTGCT PRIMER_INTERNAL_0_SEQUENCE=CAGCAACCTAAAATTGTTTTTTGA PRIMER_LEFT_0=59,22 PRIMER_RIGHT_0=145,20 PRIMER_INTERNAL_0=92,24 PRIMER_LEFT_0_TM=59.238 PRIMER_RIGHT_0_TM=59.874 PRIMER_INTERNAL_0_TM=59.607 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=29.167 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=87 PRIMER_PAIR_1_PENALTY=3.219621 PRIMER_LEFT_1_PENALTY=3.093794 PRIMER_RIGHT_1_PENALTY=0.125826 PRIMER_INTERNAL_1_PENALTY=4.253252 PRIMER_LEFT_1_SEQUENCE=GCTTCAAAGAAGTTCTTGCTCA PRIMER_RIGHT_1_SEQUENCE=ATTGCTCAGAACCCTGTGCT PRIMER_INTERNAL_1_SEQUENCE=GGAAGTTATTCATTCAGCAACC PRIMER_LEFT_1=56,22 PRIMER_RIGHT_1=145,20 PRIMER_INTERNAL_1=78,22 PRIMER_LEFT_1_TM=58.906 PRIMER_RIGHT_1_TM=59.874 PRIMER_INTERNAL_1_TM=57.747 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=9.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=90 PRIMER_PAIR_2_PENALTY=3.219621 PRIMER_LEFT_2_PENALTY=3.093794 PRIMER_RIGHT_2_PENALTY=0.125826 PRIMER_INTERNAL_2_PENALTY=4.253252 PRIMER_LEFT_2_SEQUENCE=TGCTTCAAAGAAGTTCTTGCTC PRIMER_RIGHT_2_SEQUENCE=ATTGCTCAGAACCCTGTGCT PRIMER_INTERNAL_2_SEQUENCE=GGAAGTTATTCATTCAGCAACC PRIMER_LEFT_2=55,22 PRIMER_RIGHT_2=145,20 PRIMER_INTERNAL_2=78,22 PRIMER_LEFT_2_TM=58.906 PRIMER_RIGHT_2_TM=59.874 PRIMER_INTERNAL_2_TM=57.747 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=9.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=91 PRIMER_PAIR_3_PENALTY=3.292973 PRIMER_LEFT_3_PENALTY=3.167146 PRIMER_RIGHT_3_PENALTY=0.125826 PRIMER_INTERNAL_3_PENALTY=4.392765 PRIMER_LEFT_3_SEQUENCE=GCTTCAAAGAAGTTCTTGCTCAG PRIMER_RIGHT_3_SEQUENCE=ATTGCTCAGAACCCTGTGCT PRIMER_INTERNAL_3_SEQUENCE=CAGCAACCTAAAATTGTTTTTTGA PRIMER_LEFT_3=56,23 PRIMER_RIGHT_3=145,20 PRIMER_INTERNAL_3=92,24 PRIMER_LEFT_3_TM=59.833 PRIMER_RIGHT_3_TM=59.874 PRIMER_INTERNAL_3_TM=59.607 PRIMER_LEFT_3_GC_PERCENT=43.478 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=29.167 PRIMER_LEFT_3_SELF_ANY=9.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=3.301463 PRIMER_LEFT_4_PENALTY=3.175636 PRIMER_RIGHT_4_PENALTY=0.125826 PRIMER_INTERNAL_4_PENALTY=4.253252 PRIMER_LEFT_4_SEQUENCE=TTGCTTCAAAGAAGTTCTTGCTC PRIMER_RIGHT_4_SEQUENCE=ATTGCTCAGAACCCTGTGCT PRIMER_INTERNAL_4_SEQUENCE=GGAAGTTATTCATTCAGCAACC PRIMER_LEFT_4=54,23 PRIMER_RIGHT_4=145,20 PRIMER_INTERNAL_4=78,22 PRIMER_LEFT_4_TM=60.176 PRIMER_RIGHT_4_TM=59.874 PRIMER_INTERNAL_4_TM=57.747 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=9.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=92 = SEQUENCE_ID=MH720 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTTCCAAAGAGCNTGCTTTTTAATTAGTNCAACATGCAGCAGCAAATNAGGGAAATGGGGAAGAAATTATTTGTCATCAAATTGAAATACATGTAAAGAAAACACAAGCAAAAATGTATTAAAGCCTTTTATGAGGAGGCTTTTAGTCAACATTTGGCTTGGTTAAAACCTTGAGCCAAATGTTTATTAAGGCACAGCAGTAAAGGAAGCAAAANCCATTTTTTGTACCTTT SEQUENCE_INCLUDED_REGION=36,166 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.346210 PRIMER_LEFT_0_PENALTY=1.245238 PRIMER_RIGHT_0_PENALTY=2.100972 PRIMER_INTERNAL_0_PENALTY=5.346517 PRIMER_LEFT_0_SEQUENCE=AGGGAAATGGGGAAGAAATT PRIMER_RIGHT_0_SEQUENCE=AAAAGCCTCCTCATAAAAGGC PRIMER_INTERNAL_0_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG PRIMER_LEFT_0=83,20 PRIMER_RIGHT_0=178,21 PRIMER_INTERNAL_0=126,25 PRIMER_LEFT_0_TM=58.755 PRIMER_RIGHT_0_TM=58.899 PRIMER_INTERNAL_0_TM=59.653 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_INTERNAL_0_GC_PERCENT=28.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=96 PRIMER_PAIR_1_PENALTY=3.473354 PRIMER_LEFT_1_PENALTY=1.245238 PRIMER_RIGHT_1_PENALTY=2.228115 PRIMER_INTERNAL_1_PENALTY=5.346517 PRIMER_LEFT_1_SEQUENCE=AGGGAAATGGGGAAGAAATT PRIMER_RIGHT_1_SEQUENCE=AAGCCTCCTCATAAAAGGCTTT PRIMER_INTERNAL_1_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG PRIMER_LEFT_1=83,20 PRIMER_RIGHT_1=176,22 PRIMER_INTERNAL_1=126,25 PRIMER_LEFT_1_TM=58.755 PRIMER_RIGHT_1_TM=59.772 PRIMER_INTERNAL_1_TM=59.653 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_INTERNAL_1_GC_PERCENT=28.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=94 PRIMER_PAIR_2_PENALTY=3.473354 PRIMER_LEFT_2_PENALTY=1.245238 PRIMER_RIGHT_2_PENALTY=2.228115 PRIMER_INTERNAL_2_PENALTY=5.346517 PRIMER_LEFT_2_SEQUENCE=AGGGAAATGGGGAAGAAATT PRIMER_RIGHT_2_SEQUENCE=AAAGCCTCCTCATAAAAGGCTT PRIMER_INTERNAL_2_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG PRIMER_LEFT_2=83,20 PRIMER_RIGHT_2=177,22 PRIMER_INTERNAL_2=126,25 PRIMER_LEFT_2_TM=58.755 PRIMER_RIGHT_2_TM=59.772 PRIMER_INTERNAL_2_TM=59.653 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=40.909 PRIMER_INTERNAL_2_GC_PERCENT=28.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=8.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=95 PRIMER_PAIR_3_PENALTY=3.473354 PRIMER_LEFT_3_PENALTY=1.245238 PRIMER_RIGHT_3_PENALTY=2.228115 PRIMER_INTERNAL_3_PENALTY=5.346517 PRIMER_LEFT_3_SEQUENCE=AGGGAAATGGGGAAGAAATT PRIMER_RIGHT_3_SEQUENCE=AAAAGCCTCCTCATAAAAGGCT PRIMER_INTERNAL_3_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG PRIMER_LEFT_3=83,20 PRIMER_RIGHT_3=178,22 PRIMER_INTERNAL_3=126,25 PRIMER_LEFT_3_TM=58.755 PRIMER_RIGHT_3_TM=59.772 PRIMER_INTERNAL_3_TM=59.653 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=40.909 PRIMER_INTERNAL_3_GC_PERCENT=28.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=96 PRIMER_PAIR_4_PENALTY=3.666072 PRIMER_LEFT_4_PENALTY=1.245238 PRIMER_RIGHT_4_PENALTY=2.420834 PRIMER_INTERNAL_4_PENALTY=5.346517 PRIMER_LEFT_4_SEQUENCE=AGGGAAATGGGGAAGAAATT PRIMER_RIGHT_4_SEQUENCE=GCCTCCTCATAAAAGGCTTT PRIMER_INTERNAL_4_SEQUENCE=TGTAAAGAAAACACAAGCAAAAATG PRIMER_LEFT_4=83,20 PRIMER_RIGHT_4=174,20 PRIMER_INTERNAL_4=126,25 PRIMER_LEFT_4_TM=58.755 PRIMER_RIGHT_4_TM=57.579 PRIMER_INTERNAL_4_TM=59.653 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=28.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=7.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=92 = SEQUENCE_ID=MH721 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATTGGGTNTCTCTCTCCCTACTGTGTAGAAGACCCTTACTTAATTGCTACTTCCTTAATGATGCCTTTCCCAGGACAAAACTCCATGGTGACTTTGTGTTGCTCTGATAGCCCTTCTCATGGGTGGTAACTCTGTCATTTGTTTTCCATCTGTCCATCCATTCCACAGACAGTGATTACAACCAGGATTGCTGCTGGGCTGTGAAAATACTAAAAACAATGAGGCAATGTGTCTACCTTCAAGAGGTTTACAGTCTATAATCTGTGCTATACTGTAATGATTTCTGTGACTGCGTTTGATATTTCTAG SEQUENCE_INCLUDED_REGION=36,311 P3_COMMENT=CL is AGGACAAAACTCCATGGTGA P3_COMMENT=CR is AAACGCAGTCACAGAAATCA P3_COMMENT=CM is GTGTTGCTCTGATAGCCCTT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.488969 PRIMER_LEFT_0_PENALTY=0.353069 PRIMER_RIGHT_0_PENALTY=0.135900 PRIMER_INTERNAL_0_PENALTY=0.527169 PRIMER_LEFT_0_SEQUENCE=CTGATAGCCCTTCTCATGGG PRIMER_RIGHT_0_SEQUENCE=CAGCAGCAATCCTGGTTGTA PRIMER_INTERNAL_0_SEQUENCE=TCCATCCATTCCACAGACAG PRIMER_LEFT_0=142,20 PRIMER_RIGHT_0=234,20 PRIMER_INTERNAL_0=191,20 PRIMER_LEFT_0_TM=59.647 PRIMER_RIGHT_0_TM=59.864 PRIMER_INTERNAL_0_TM=59.473 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.6000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=93 PRIMER_PAIR_1_PENALTY=0.535385 PRIMER_LEFT_1_PENALTY=0.008216 PRIMER_RIGHT_1_PENALTY=0.527169 PRIMER_INTERNAL_1_PENALTY=0.353069 PRIMER_LEFT_1_SEQUENCE=CCATGGTGACTTTGTGTTGC PRIMER_RIGHT_1_SEQUENCE=CTGTGGAATGGATGGACAGA PRIMER_INTERNAL_1_SEQUENCE=CTGATAGCCCTTCTCATGGG PRIMER_LEFT_1=121,20 PRIMER_RIGHT_1=206,20 PRIMER_INTERNAL_1=142,20 PRIMER_LEFT_1_TM=60.008 PRIMER_RIGHT_1_TM=59.473 PRIMER_INTERNAL_1_TM=59.647 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=86 PRIMER_PAIR_2_PENALTY=0.535385 PRIMER_LEFT_2_PENALTY=0.008216 PRIMER_RIGHT_2_PENALTY=0.527169 PRIMER_INTERNAL_2_PENALTY=0.353069 PRIMER_LEFT_2_SEQUENCE=CCATGGTGACTTTGTGTTGC PRIMER_RIGHT_2_SEQUENCE=TCTGTGGAATGGATGGACAG PRIMER_INTERNAL_2_SEQUENCE=CTGATAGCCCTTCTCATGGG PRIMER_LEFT_2=121,20 PRIMER_RIGHT_2=207,20 PRIMER_INTERNAL_2=142,20 PRIMER_LEFT_2_TM=60.008 PRIMER_RIGHT_2_TM=59.473 PRIMER_INTERNAL_2_TM=59.647 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=87 PRIMER_PAIR_3_PENALTY=0.535385 PRIMER_LEFT_3_PENALTY=0.008216 PRIMER_RIGHT_3_PENALTY=0.527169 PRIMER_INTERNAL_3_PENALTY=0.353069 PRIMER_LEFT_3_SEQUENCE=CCATGGTGACTTTGTGTTGC PRIMER_RIGHT_3_SEQUENCE=CTGTCTGTGGAATGGATGGA PRIMER_INTERNAL_3_SEQUENCE=CTGATAGCCCTTCTCATGGG PRIMER_LEFT_3=121,20 PRIMER_RIGHT_3=210,20 PRIMER_INTERNAL_3=142,20 PRIMER_LEFT_3_TM=60.008 PRIMER_RIGHT_3_TM=59.473 PRIMER_INTERNAL_3_TM=59.647 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=0.628382 PRIMER_LEFT_4_PENALTY=0.492482 PRIMER_RIGHT_4_PENALTY=0.135900 PRIMER_INTERNAL_4_PENALTY=0.527169 PRIMER_LEFT_4_SEQUENCE=GATAGCCCTTCTCATGGGTG PRIMER_RIGHT_4_SEQUENCE=CAGCAGCAATCCTGGTTGTA PRIMER_INTERNAL_4_SEQUENCE=TCCATCCATTCCACAGACAG PRIMER_LEFT_4=144,20 PRIMER_RIGHT_4=234,20 PRIMER_INTERNAL_4=191,20 PRIMER_LEFT_4_TM=59.508 PRIMER_RIGHT_4_TM=59.864 PRIMER_INTERNAL_4_TM=59.473 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH724 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCTGCAGANCTGGGGTTCTGATGGTTAGGATGAGCCACTTGGNNATAGATGATAGGTGAGCCATTCTAAAATCATAAAAACAAAAATCAATTCAATGAACTTAGTGAATTCATCTTACTCAGTCTGTCTTATTAAGTCTCAATGCCCCCAGGTCCTTTGCAAAAACAATGCTCATTTTGACTGCACCCTCCAGAAAAAAAGTTGCAGGAGAACTTTTATTTTGTTTCCAAGCCCACTGAAGGTAAGTTTTCTGTTTTGTATCTCAATACCTTTAACCCAAAAAGGAGTTCCCATTTCATTCCTCCTACATG SEQUENCE_INCLUDED_REGION=36,311 P3_COMMENT=CL is CTGGGGTTCTGATGGTTAGG P3_COMMENT=CR is TGTAGGAGGAATGAAATGGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.534534 PRIMER_LEFT_0_PENALTY=0.262854 PRIMER_RIGHT_0_PENALTY=0.271680 PRIMER_INTERNAL_0_PENALTY=1.995898 PRIMER_LEFT_0_SEQUENCE=GCTCATTTTGACTGCACCCT PRIMER_RIGHT_0_SEQUENCE=CTTACCTTCAGTGGGCTTGG PRIMER_INTERNAL_0_SEQUENCE=CCAGAAAAAAAGTTGCAGGAG PRIMER_LEFT_0=205,20 PRIMER_RIGHT_0=281,20 PRIMER_INTERNAL_0=225,21 PRIMER_LEFT_0_TM=60.263 PRIMER_RIGHT_0_TM=59.728 PRIMER_INTERNAL_0_TM=59.004 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.1000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=0.889270 PRIMER_LEFT_1_PENALTY=0.262854 PRIMER_RIGHT_1_PENALTY=0.626415 PRIMER_INTERNAL_1_PENALTY=1.995898 PRIMER_LEFT_1_SEQUENCE=GCTCATTTTGACTGCACCCT PRIMER_RIGHT_1_SEQUENCE=TACCTTCAGTGGGCTTGGAA PRIMER_INTERNAL_1_SEQUENCE=CCAGAAAAAAAGTTGCAGGAG PRIMER_LEFT_1=205,20 PRIMER_RIGHT_1=279,20 PRIMER_INTERNAL_1=225,21 PRIMER_LEFT_1_TM=60.263 PRIMER_RIGHT_1_TM=60.626 PRIMER_INTERNAL_1_TM=59.004 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.1000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=0.889270 PRIMER_LEFT_2_PENALTY=0.262854 PRIMER_RIGHT_2_PENALTY=0.626415 PRIMER_INTERNAL_2_PENALTY=1.995898 PRIMER_LEFT_2_SEQUENCE=GCTCATTTTGACTGCACCCT PRIMER_RIGHT_2_SEQUENCE=TTACCTTCAGTGGGCTTGGA PRIMER_INTERNAL_2_SEQUENCE=CCAGAAAAAAAGTTGCAGGAG PRIMER_LEFT_2=205,20 PRIMER_RIGHT_2=280,20 PRIMER_INTERNAL_2=225,21 PRIMER_LEFT_2_TM=60.263 PRIMER_RIGHT_2_TM=60.626 PRIMER_INTERNAL_2_TM=59.004 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.1000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=76 PRIMER_PAIR_3_PENALTY=1.188227 PRIMER_LEFT_3_PENALTY=1.029480 PRIMER_RIGHT_3_PENALTY=0.158747 PRIMER_INTERNAL_3_PENALTY=8.725741 PRIMER_LEFT_3_SEQUENCE=TTTTGTTTCCAAGCCCACTG PRIMER_RIGHT_3_SEQUENCE=AATGGGAACTCCTTTTTGGG PRIMER_INTERNAL_3_SEQUENCE=GGTAAGTTTTCTGTTTTGTATCTCAA PRIMER_LEFT_3=254,20 PRIMER_RIGHT_3=330,20 PRIMER_INTERNAL_3=276,26 PRIMER_LEFT_3_TM=61.029 PRIMER_RIGHT_3_TM=60.159 PRIMER_INTERNAL_3_TM=57.274 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=30.769 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=77 PRIMER_PAIR_4_PENALTY=1.219518 PRIMER_LEFT_4_PENALTY=0.066323 PRIMER_RIGHT_4_PENALTY=1.153194 PRIMER_INTERNAL_4_PENALTY=1.132549 PRIMER_LEFT_4_SEQUENCE=TTAAGTCTCAATGCCCCCAG PRIMER_RIGHT_4_SEQUENCE=TCCTGCAACTTTTTTTCTGGA PRIMER_INTERNAL_4_SEQUENCE=AATGCTCATTTTGACTGCACC PRIMER_LEFT_4=167,20 PRIMER_RIGHT_4=244,21 PRIMER_INTERNAL_4=202,21 PRIMER_LEFT_4_TM=60.066 PRIMER_RIGHT_4_TM=59.847 PRIMER_INTERNAL_4_TM=60.133 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_INTERNAL_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=78 = SEQUENCE_ID=MH730 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGGGAACCCTATAACTGATGTGTGCTGTTTTGAGAACAAAATACCTTCTCTTTGGAGTTCTGGCTTTAGAGGANATTGATAGAACTAAAAGTACATTTGACATGTTCATTACTCAGGGAAATTAATCCATTATGGCAATTAGTGCACTCTTTTTGCACACTGAGGTCTAAATACCAACTGAAAGAAGTTATAAAGATGTGATGTTAATGAGTNTTGTGAGGTTGTCTTTTCTCAAAGAAAACTGAGTACATGAATTTAATAAAAATATTCAG SEQUENCE_INCLUDED_REGION=35,175 P3_COMMENT=CL is ATGTGTGCTGTTTTGAGAACA P3_COMMENT=CR is TCAGTGTGCAAAAAGAGTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.480898 PRIMER_LEFT_0_PENALTY=1.367716 PRIMER_RIGHT_0_PENALTY=2.113182 PRIMER_INTERNAL_0_PENALTY=8.777675 PRIMER_LEFT_0_SEQUENCE=CCTTCTCTTTGGAGTTCTGGC PRIMER_RIGHT_0_SEQUENCE=TGCCATAATGGATTAATTTCCC PRIMER_INTERNAL_0_SEQUENCE=AAGTACATTTGACATGTTCATTACTCA PRIMER_LEFT_0=82,21 PRIMER_RIGHT_0=175,22 PRIMER_INTERNAL_0=127,27 PRIMER_LEFT_0_TM=60.368 PRIMER_RIGHT_0_TM=59.887 PRIMER_INTERNAL_0_TM=58.222 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=36.364 PRIMER_INTERNAL_0_GC_PERCENT=29.630 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=94 PRIMER_PAIR_1_PENALTY=3.894783 PRIMER_LEFT_1_PENALTY=1.937220 PRIMER_RIGHT_1_PENALTY=1.957563 PRIMER_INTERNAL_1_PENALTY=3.515201 PRIMER_LEFT_1_SEQUENCE=TGGAGTTCTGGCTTTAGAGGA PRIMER_RIGHT_1_SEQUENCE=TGCACTAATTGCCATAATGGA PRIMER_INTERNAL_1_SEQUENCE=TGACATGTTCATTACTCAGGGAA PRIMER_LEFT_1=91,21 PRIMER_RIGHT_1=184,21 PRIMER_INTERNAL_1=136,23 PRIMER_LEFT_1_TM=59.063 PRIMER_RIGHT_1_TM=59.042 PRIMER_INTERNAL_1_TM=59.485 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=38.095 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=94 PRIMER_PAIR_2_PENALTY=4.050402 PRIMER_LEFT_2_PENALTY=1.937220 PRIMER_RIGHT_2_PENALTY=2.113182 PRIMER_INTERNAL_2_PENALTY=8.777675 PRIMER_LEFT_2_SEQUENCE=TGGAGTTCTGGCTTTAGAGGA PRIMER_RIGHT_2_SEQUENCE=TGCCATAATGGATTAATTTCCC PRIMER_INTERNAL_2_SEQUENCE=AAGTACATTTGACATGTTCATTACTCA PRIMER_LEFT_2=91,21 PRIMER_RIGHT_2=175,22 PRIMER_INTERNAL_2=127,27 PRIMER_LEFT_2_TM=59.063 PRIMER_RIGHT_2_TM=59.887 PRIMER_INTERNAL_2_TM=58.222 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=36.364 PRIMER_INTERNAL_2_GC_PERCENT=29.630 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=85 PRIMER_PAIR_3_PENALTY=4.071366 PRIMER_LEFT_3_PENALTY=2.113803 PRIMER_RIGHT_3_PENALTY=1.957563 PRIMER_INTERNAL_3_PENALTY=3.515201 PRIMER_LEFT_3_SEQUENCE=TTTGGAGTTCTGGCTTTAGAGG PRIMER_RIGHT_3_SEQUENCE=TGCACTAATTGCCATAATGGA PRIMER_INTERNAL_3_SEQUENCE=TGACATGTTCATTACTCAGGGAA PRIMER_LEFT_3=89,22 PRIMER_RIGHT_3=184,21 PRIMER_INTERNAL_3=136,23 PRIMER_LEFT_3_TM=59.886 PRIMER_RIGHT_3_TM=59.042 PRIMER_INTERNAL_3_TM=59.485 PRIMER_LEFT_3_GC_PERCENT=45.455 PRIMER_RIGHT_3_GC_PERCENT=38.095 PRIMER_INTERNAL_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=96 PRIMER_PAIR_4_PENALTY=4.077829 PRIMER_LEFT_4_PENALTY=1.937220 PRIMER_RIGHT_4_PENALTY=2.140609 PRIMER_INTERNAL_4_PENALTY=3.515201 PRIMER_LEFT_4_SEQUENCE=TGGAGTTCTGGCTTTAGAGGA PRIMER_RIGHT_4_SEQUENCE=GTGCACTAATTGCCATAATGGA PRIMER_INTERNAL_4_SEQUENCE=TGACATGTTCATTACTCAGGGAA PRIMER_LEFT_4=91,21 PRIMER_RIGHT_4=185,22 PRIMER_INTERNAL_4=136,23 PRIMER_LEFT_4_TM=59.063 PRIMER_RIGHT_4_TM=59.859 PRIMER_INTERNAL_4_TM=59.485 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=40.909 PRIMER_INTERNAL_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=95 = SEQUENCE_ID=MH733 SEQUENCE_TEMPLATE=TTTTTACTTGTTTTCAGTATTCTTTAAGCCAGGAAAGTATGCCTAGTCTCAGAATTGTTTAACATAAAGACACAGAATTACGTTGGTTTCAATCCTCATGACATCGAGGGAAAAAAACCCAAATTAATATCTCAGAAAGTTATCCTGCCACTGAAGTATTAGCAAAAGTCCTTCCCATTCCATCTAGCCTTTCCATAAATGAAACTTCTACCACTTTCAGGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACG SEQUENCE_INCLUDED_REGION=0,220 P3_COMMENT=CL is TTTTCAGTATTCTTTAAGCCAGG P3_COMMENT=CR is AAAGTGGTAGAAGTTTCATTTATGG P3_COMMENT=CM is ATGCCTAGTCTCAGAATTGTTTAAC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.210551 PRIMER_LEFT_0_PENALTY=0.113696 PRIMER_RIGHT_0_PENALTY=0.096855 PRIMER_INTERNAL_0_PENALTY=2.621889 PRIMER_LEFT_0_SEQUENCE=AATCCTCATGACATCGAGGG PRIMER_RIGHT_0_SEQUENCE=TGGAATGGGAAGGACTTTTG PRIMER_INTERNAL_0_SEQUENCE=CAGAAAGTTATCCTGCCACTGA PRIMER_LEFT_0=90,20 PRIMER_RIGHT_0=181,20 PRIMER_INTERNAL_0=132,22 PRIMER_LEFT_0_TM=59.886 PRIMER_RIGHT_0_TM=59.903 PRIMER_INTERNAL_0_TM=59.378 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=7.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=92 PRIMER_PAIR_1_PENALTY=0.253412 PRIMER_LEFT_1_PENALTY=0.156557 PRIMER_RIGHT_1_PENALTY=0.096855 PRIMER_INTERNAL_1_PENALTY=2.621889 PRIMER_LEFT_1_SEQUENCE=CGAGGGAAAAAAACCCAAAT PRIMER_RIGHT_1_SEQUENCE=TGGAATGGGAAGGACTTTTG PRIMER_INTERNAL_1_SEQUENCE=CAGAAAGTTATCCTGCCACTGA PRIMER_LEFT_1=104,20 PRIMER_RIGHT_1=181,20 PRIMER_INTERNAL_1=132,22 PRIMER_LEFT_1_TM=60.157 PRIMER_RIGHT_1_TM=59.903 PRIMER_INTERNAL_1_TM=59.378 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=0.262897 PRIMER_LEFT_2_PENALTY=0.166042 PRIMER_RIGHT_2_PENALTY=0.096855 PRIMER_INTERNAL_2_PENALTY=2.621889 PRIMER_LEFT_2_SEQUENCE=ACATCGAGGGAAAAAAACCC PRIMER_RIGHT_2_SEQUENCE=TGGAATGGGAAGGACTTTTG PRIMER_INTERNAL_2_SEQUENCE=CAGAAAGTTATCCTGCCACTGA PRIMER_LEFT_2=100,20 PRIMER_RIGHT_2=181,20 PRIMER_INTERNAL_2=132,22 PRIMER_LEFT_2_TM=60.166 PRIMER_RIGHT_2_TM=59.903 PRIMER_INTERNAL_2_TM=59.378 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=82 PRIMER_PAIR_3_PENALTY=0.535576 PRIMER_LEFT_3_PENALTY=0.438721 PRIMER_RIGHT_3_PENALTY=0.096855 PRIMER_INTERNAL_3_PENALTY=2.621889 PRIMER_LEFT_3_SEQUENCE=ATCCTCATGACATCGAGGGA PRIMER_RIGHT_3_SEQUENCE=TGGAATGGGAAGGACTTTTG PRIMER_INTERNAL_3_SEQUENCE=CAGAAAGTTATCCTGCCACTGA PRIMER_LEFT_3=91,20 PRIMER_RIGHT_3=181,20 PRIMER_INTERNAL_3=132,22 PRIMER_LEFT_3_TM=60.439 PRIMER_RIGHT_3_TM=59.903 PRIMER_INTERNAL_3_TM=59.378 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.455 PRIMER_LEFT_3_SELF_ANY=9.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=7.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=91 PRIMER_PAIR_4_PENALTY=0.740946 PRIMER_LEFT_4_PENALTY=0.644091 PRIMER_RIGHT_4_PENALTY=0.096855 PRIMER_INTERNAL_4_PENALTY=2.621889 PRIMER_LEFT_4_SEQUENCE=ATCGAGGGAAAAAAACCCAA PRIMER_RIGHT_4_SEQUENCE=TGGAATGGGAAGGACTTTTG PRIMER_INTERNAL_4_SEQUENCE=CAGAAAGTTATCCTGCCACTGA PRIMER_LEFT_4=102,20 PRIMER_RIGHT_4=181,20 PRIMER_INTERNAL_4=132,22 PRIMER_LEFT_4_TM=60.644 PRIMER_RIGHT_4_TM=59.903 PRIMER_INTERNAL_4_TM=59.378 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.455 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH736 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTCCTTGACATCAACATCAGAAGTNAGGGACTTACCCTAACATCCTTGGCTTTAATAATCCATGACGGAGGTTTCATTCTTTAATCTAAACTTTTGTGGAAGNTATTCATAGTTTNTTCTCTACCGCNCTTGTAGNGATAATTAAATTGTACACATTGATTACCACTTTGCATTCAGTCATACTTTCTTTTAAAATAATAGTAACTAACATTTACATAGTGATTACCATATGCTATGCATTGTGCTAAGCACTTTACATTTCTTAAATCATTTAATATTCAGGAC SEQUENCE_INCLUDED_REGION=36,286 P3_COMMENT=CL is TGGCTTTAATAATCCATGACG P3_COMMENT=CR is GCTTAGCACAATGCATAGCA P3_COMMENT=CM is ATTGTACACATTGATTACCACTTTG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.178522 PRIMER_LEFT_0_PENALTY=2.861164 PRIMER_RIGHT_0_PENALTY=0.317359 PRIMER_INTERNAL_0_PENALTY=2.331778 PRIMER_LEFT_0_SEQUENCE=TGTCCTTGACATCAACATCAGA PRIMER_RIGHT_0_SEQUENCE=TGAAACCTCCGTCATGGATT PRIMER_INTERNAL_0_SEQUENCE=ACCCTAACATCCTTGGCTTT PRIMER_LEFT_0=36,22 PRIMER_RIGHT_0=112,20 PRIMER_INTERNAL_0=70,20 PRIMER_LEFT_0_TM=59.139 PRIMER_RIGHT_0_TM=60.317 PRIMER_INTERNAL_0_TM=57.668 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=3.178522 PRIMER_LEFT_1_PENALTY=2.861164 PRIMER_RIGHT_1_PENALTY=0.317359 PRIMER_INTERNAL_1_PENALTY=2.331778 PRIMER_LEFT_1_SEQUENCE=TGTCCTTGACATCAACATCAGA PRIMER_RIGHT_1_SEQUENCE=AATGAAACCTCCGTCATGGA PRIMER_INTERNAL_1_SEQUENCE=ACCCTAACATCCTTGGCTTT PRIMER_LEFT_1=36,22 PRIMER_RIGHT_1=114,20 PRIMER_INTERNAL_1=70,20 PRIMER_LEFT_1_TM=59.139 PRIMER_RIGHT_1_TM=60.317 PRIMER_INTERNAL_1_TM=57.668 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=79 PRIMER_PAIR_2_PENALTY=3.178522 PRIMER_LEFT_2_PENALTY=2.861164 PRIMER_RIGHT_2_PENALTY=0.317359 PRIMER_INTERNAL_2_PENALTY=2.331778 PRIMER_LEFT_2_SEQUENCE=TGTCCTTGACATCAACATCAGA PRIMER_RIGHT_2_SEQUENCE=GAATGAAACCTCCGTCATGG PRIMER_INTERNAL_2_SEQUENCE=ACCCTAACATCCTTGGCTTT PRIMER_LEFT_2=36,22 PRIMER_RIGHT_2=115,20 PRIMER_INTERNAL_2=70,20 PRIMER_LEFT_2_TM=59.139 PRIMER_RIGHT_2_TM=60.317 PRIMER_INTERNAL_2_TM=57.668 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=8.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=3.643566 PRIMER_LEFT_3_PENALTY=2.861164 PRIMER_RIGHT_3_PENALTY=0.782403 PRIMER_INTERNAL_3_PENALTY=2.331778 PRIMER_LEFT_3_SEQUENCE=TGTCCTTGACATCAACATCAGA PRIMER_RIGHT_3_SEQUENCE=ATGAAACCTCCGTCATGGAT PRIMER_INTERNAL_3_SEQUENCE=ACCCTAACATCCTTGGCTTT PRIMER_LEFT_3=36,22 PRIMER_RIGHT_3=113,20 PRIMER_INTERNAL_3=70,20 PRIMER_LEFT_3_TM=59.139 PRIMER_RIGHT_3_TM=59.218 PRIMER_INTERNAL_3_TM=57.668 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=78 PRIMER_PAIR_4_PENALTY=3.861466 PRIMER_LEFT_4_PENALTY=3.544107 PRIMER_RIGHT_4_PENALTY=0.317359 PRIMER_INTERNAL_4_PENALTY=2.331778 PRIMER_LEFT_4_SEQUENCE=TGTCCTTGACATCAACATCAGAA PRIMER_RIGHT_4_SEQUENCE=TGAAACCTCCGTCATGGATT PRIMER_INTERNAL_4_SEQUENCE=ACCCTAACATCCTTGGCTTT PRIMER_LEFT_4=36,23 PRIMER_RIGHT_4=112,20 PRIMER_INTERNAL_4=70,20 PRIMER_LEFT_4_TM=60.544 PRIMER_RIGHT_4_TM=60.317 PRIMER_INTERNAL_4_TM=57.668 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=77 = SEQUENCE_ID=MH739 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTTAGAGGCTGTATGGCCTCCAGGAAAAGTAGCTTTAATGCACTGCAGATGGCACCAAAAGTCAGGGACACCAAAAACCAAAAGAAACAGAAAGGCAGACAGAGAGGCAAAGAGGGGAGCAATGATTGCATCACATTTTAAAGAGGAAGCCTTAGGGTACCGANGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCT SEQUENCE_INCLUDED_REGION=36,156 P3_COMMENT=CL is GTATGGCCTCCAGGAAAAGT P3_COMMENT=CR is TTAAAATGTGATGCAATCATTG P3_COMMENT=CM is GCTTTAATGCACTGCAGATG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.285274 PRIMER_LEFT_0_PENALTY=0.151533 PRIMER_RIGHT_0_PENALTY=0.133741 PRIMER_INTERNAL_0_PENALTY=0.111077 PRIMER_LEFT_0_SEQUENCE=GGCCTCCAGGAAAAGTAGCT PRIMER_RIGHT_0_SEQUENCE=TTGCCTCTCTGTCTGCCTTT PRIMER_INTERNAL_0_SEQUENCE=AGATGGCACCAAAAGTCAGG PRIMER_LEFT_0=52,20 PRIMER_RIGHT_0=147,20 PRIMER_INTERNAL_0=84,20 PRIMER_LEFT_0_TM=59.848 PRIMER_RIGHT_0_TM=60.134 PRIMER_INTERNAL_0_TM=60.111 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=96 PRIMER_PAIR_1_PENALTY=0.285274 PRIMER_LEFT_1_PENALTY=0.151533 PRIMER_RIGHT_1_PENALTY=0.133741 PRIMER_INTERNAL_1_PENALTY=0.111077 PRIMER_LEFT_1_SEQUENCE=GGCCTCCAGGAAAAGTAGCT PRIMER_RIGHT_1_SEQUENCE=TTTGCCTCTCTGTCTGCCTT PRIMER_INTERNAL_1_SEQUENCE=AGATGGCACCAAAAGTCAGG PRIMER_LEFT_1=52,20 PRIMER_RIGHT_1=148,20 PRIMER_INTERNAL_1=84,20 PRIMER_LEFT_1_TM=59.848 PRIMER_RIGHT_1_TM=60.134 PRIMER_INTERNAL_1_TM=60.111 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=97 PRIMER_PAIR_2_PENALTY=0.294569 PRIMER_LEFT_2_PENALTY=0.160828 PRIMER_RIGHT_2_PENALTY=0.133741 PRIMER_INTERNAL_2_PENALTY=0.399325 PRIMER_LEFT_2_SEQUENCE=TTTAATGCACTGCAGATGGC PRIMER_RIGHT_2_SEQUENCE=TTGCCTCTCTGTCTGCCTTT PRIMER_INTERNAL_2_SEQUENCE=ACCAAAAGTCAGGGACACCA PRIMER_LEFT_2=71,20 PRIMER_RIGHT_2=147,20 PRIMER_INTERNAL_2=91,20 PRIMER_LEFT_2_TM=59.839 PRIMER_RIGHT_2_TM=60.134 PRIMER_INTERNAL_2_TM=60.399 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.6000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=0.294569 PRIMER_LEFT_3_PENALTY=0.160828 PRIMER_RIGHT_3_PENALTY=0.133741 PRIMER_INTERNAL_3_PENALTY=0.399325 PRIMER_LEFT_3_SEQUENCE=TTTAATGCACTGCAGATGGC PRIMER_RIGHT_3_SEQUENCE=TTTGCCTCTCTGTCTGCCTT PRIMER_INTERNAL_3_SEQUENCE=ACCAAAAGTCAGGGACACCA PRIMER_LEFT_3=71,20 PRIMER_RIGHT_3=148,20 PRIMER_INTERNAL_3=91,20 PRIMER_LEFT_3_TM=59.839 PRIMER_RIGHT_3_TM=60.134 PRIMER_INTERNAL_3_TM=60.399 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.6000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=78 PRIMER_PAIR_4_PENALTY=0.406531 PRIMER_LEFT_4_PENALTY=0.151533 PRIMER_RIGHT_4_PENALTY=0.254998 PRIMER_INTERNAL_4_PENALTY=0.111077 PRIMER_LEFT_4_SEQUENCE=GGCCTCCAGGAAAAGTAGCT PRIMER_RIGHT_4_SEQUENCE=CTTTGCCTCTCTGTCTGCCT PRIMER_INTERNAL_4_SEQUENCE=AGATGGCACCAAAAGTCAGG PRIMER_LEFT_4=52,20 PRIMER_RIGHT_4=149,20 PRIMER_INTERNAL_4=84,20 PRIMER_LEFT_4_TM=59.848 PRIMER_RIGHT_4_TM=59.745 PRIMER_INTERNAL_4_TM=60.111 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH745 SEQUENCE_TEMPLATE=GCATGCCCTGCTCAGACTGTGCACTACAGACCAATCAACTTGAGCAGTTCAGAGAACAAAATAGTCAATGTTAGCCTGGCAGATCTTAGAGGTNGAAATCGCCCCAATACAGGGCCCTTATACACAGAGGCCGATCGAGTCATACAGAGAACAAATTCAATGCAGCAGTTGGAACAGTGGATTAAAATCCAGAAGGGGAGGGGTCATGAAGANGAAACCAGGGGGTAAGTGTCTGTCTTGTCATTATGAACCCAGGTTTCTTTGATGCTGTGTTGGTGGAGATTGGTTTCTGAG SEQUENCE_INCLUDED_REGION=0,294 P3_COMMENT=CL is AGTCAATGTTAGCCTGGCAG P3_COMMENT=CR is AATCTCCACCAACACAGCAT P3_COMMENT=CM is GAAATCGCCCCAATACAGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.338549 PRIMER_LEFT_0_PENALTY=0.088413 PRIMER_RIGHT_0_PENALTY=0.250137 PRIMER_INTERNAL_0_PENALTY=0.095488 PRIMER_LEFT_0_SEQUENCE=CCCTTATACACAGAGGCCGA PRIMER_RIGHT_0_SEQUENCE=CCCTCCCCTTCTGGATTTTA PRIMER_INTERNAL_0_SEQUENCE=ATGCAGCAGTTGGAACAGTG PRIMER_LEFT_0=114,20 PRIMER_RIGHT_0=201,20 PRIMER_INTERNAL_0=159,20 PRIMER_LEFT_0_TM=60.088 PRIMER_RIGHT_0_TM=60.250 PRIMER_INTERNAL_0_TM=59.905 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=11.4000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 PRIMER_PAIR_1_PENALTY=0.401678 PRIMER_LEFT_1_PENALTY=0.250137 PRIMER_RIGHT_1_PENALTY=0.151542 PRIMER_INTERNAL_1_PENALTY=0.575181 PRIMER_LEFT_1_SEQUENCE=TAAAATCCAGAAGGGGAGGG PRIMER_RIGHT_1_SEQUENCE=CCACCAACACAGCATCAAAG PRIMER_INTERNAL_1_SEQUENCE=CCAGGGGGTAAGTGTCTGTC PRIMER_LEFT_1=182,20 PRIMER_RIGHT_1=278,20 PRIMER_INTERNAL_1=217,20 PRIMER_LEFT_1_TM=60.250 PRIMER_RIGHT_1_TM=60.152 PRIMER_INTERNAL_1_TM=59.425 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.3000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=97 PRIMER_PAIR_2_PENALTY=0.490609 PRIMER_LEFT_2_PENALTY=0.240472 PRIMER_RIGHT_2_PENALTY=0.250137 PRIMER_INTERNAL_2_PENALTY=0.095488 PRIMER_LEFT_2_SEQUENCE=AGGCCGATCGAGTCATACAG PRIMER_RIGHT_2_SEQUENCE=CCCTCCCCTTCTGGATTTTA PRIMER_INTERNAL_2_SEQUENCE=ATGCAGCAGTTGGAACAGTG PRIMER_LEFT_2=127,20 PRIMER_RIGHT_2=201,20 PRIMER_INTERNAL_2=159,20 PRIMER_LEFT_2_TM=60.240 PRIMER_RIGHT_2_TM=60.250 PRIMER_INTERNAL_2_TM=59.905 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=5.7000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=0.490609 PRIMER_LEFT_3_PENALTY=0.240472 PRIMER_RIGHT_3_PENALTY=0.250137 PRIMER_INTERNAL_3_PENALTY=0.095488 PRIMER_LEFT_3_SEQUENCE=ATACACAGAGGCCGATCGAG PRIMER_RIGHT_3_SEQUENCE=CCCTCCCCTTCTGGATTTTA PRIMER_INTERNAL_3_SEQUENCE=ATGCAGCAGTTGGAACAGTG PRIMER_LEFT_3=119,20 PRIMER_RIGHT_3=201,20 PRIMER_INTERNAL_3=159,20 PRIMER_LEFT_3_TM=60.240 PRIMER_RIGHT_3_TM=60.250 PRIMER_INTERNAL_3_TM=59.905 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.4000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=0.670668 PRIMER_LEFT_4_PENALTY=0.095488 PRIMER_RIGHT_4_PENALTY=0.575181 PRIMER_INTERNAL_4_PENALTY=0.250137 PRIMER_LEFT_4_SEQUENCE=ATGCAGCAGTTGGAACAGTG PRIMER_RIGHT_4_SEQUENCE=GACAGACACTTACCCCCTGG PRIMER_INTERNAL_4_SEQUENCE=TAAAATCCAGAAGGGGAGGG PRIMER_LEFT_4=159,20 PRIMER_RIGHT_4=236,20 PRIMER_INTERNAL_4=182,20 PRIMER_LEFT_4_TM=59.905 PRIMER_RIGHT_4_TM=59.425 PRIMER_INTERNAL_4_TM=60.250 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=78 = SEQUENCE_ID=MH750 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACAGGCAAGGGCCACACCCAGAAGAGGAGGGAGGTGGACTTCAAGTGGCCTCGTAAAACAAAGACACATTCTCCTATAATGTTCAGTCATTCGGGATGTGTTTTAGACTAAGGAAGATTTCCACTCCAGGTCCTGCCAGAGNTGCTGTGAGAAACTATCACGCTGGTCCTCTCATCTGGATCACCAGACAACAG SEQUENCE_INCLUDED_REGION=33,196 P3_COMMENT=CL is CTTCAAGTGGCCTCGTAAAA P3_COMMENT=CR is CTGGTGATCCAGATGAGAGG P3_COMMENT=CM is CACATTCTCCTATAATGTTCAGTCA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.749424 PRIMER_LEFT_0_PENALTY=0.254613 PRIMER_RIGHT_0_PENALTY=0.494811 PRIMER_INTERNAL_0_PENALTY=1.067229 PRIMER_LEFT_0_SEQUENCE=GGACTTCAAGTGGCCTCGTA PRIMER_RIGHT_0_SEQUENCE=CAGGACCTGGAGTGGAAATC PRIMER_INTERNAL_0_SEQUENCE=TCAGTCATTCGGGATGTGTT PRIMER_LEFT_0=70,20 PRIMER_RIGHT_0=169,20 PRIMER_INTERNAL_0=117,20 PRIMER_LEFT_0_TM=60.255 PRIMER_RIGHT_0_TM=59.505 PRIMER_INTERNAL_0_TM=58.933 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.4000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=1.177496 PRIMER_LEFT_1_PENALTY=0.682685 PRIMER_RIGHT_1_PENALTY=0.494811 PRIMER_INTERNAL_1_PENALTY=1.067229 PRIMER_LEFT_1_SEQUENCE=TCAAGTGGCCTCGTAAAACA PRIMER_RIGHT_1_SEQUENCE=CAGGACCTGGAGTGGAAATC PRIMER_INTERNAL_1_SEQUENCE=TCAGTCATTCGGGATGTGTT PRIMER_LEFT_1=75,20 PRIMER_RIGHT_1=169,20 PRIMER_INTERNAL_1=117,20 PRIMER_LEFT_1_TM=59.317 PRIMER_RIGHT_1_TM=59.505 PRIMER_INTERNAL_1_TM=58.933 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=95 PRIMER_PAIR_2_PENALTY=1.298512 PRIMER_LEFT_2_PENALTY=1.067229 PRIMER_RIGHT_2_PENALTY=0.231283 PRIMER_INTERNAL_2_PENALTY=0.494811 PRIMER_LEFT_2_SEQUENCE=TCAGTCATTCGGGATGTGTT PRIMER_RIGHT_2_SEQUENCE=GATGAGAGGACCAGCGTGAT PRIMER_INTERNAL_2_SEQUENCE=GATTTCCACTCCAGGTCCTG PRIMER_LEFT_2=117,20 PRIMER_RIGHT_2=210,20 PRIMER_INTERNAL_2=150,20 PRIMER_LEFT_2_TM=58.933 PRIMER_RIGHT_2_TM=60.231 PRIMER_INTERNAL_2_TM=59.505 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=7.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.3000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=94 PRIMER_PAIR_3_PENALTY=1.298512 PRIMER_LEFT_3_PENALTY=1.067229 PRIMER_RIGHT_3_PENALTY=0.231283 PRIMER_INTERNAL_3_PENALTY=0.494811 PRIMER_LEFT_3_SEQUENCE=TTCAGTCATTCGGGATGTGT PRIMER_RIGHT_3_SEQUENCE=GATGAGAGGACCAGCGTGAT PRIMER_INTERNAL_3_SEQUENCE=GATTTCCACTCCAGGTCCTG PRIMER_LEFT_3=116,20 PRIMER_RIGHT_3=210,20 PRIMER_INTERNAL_3=150,20 PRIMER_LEFT_3_TM=58.933 PRIMER_RIGHT_3_TM=60.231 PRIMER_INTERNAL_3_TM=59.505 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=7.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.3000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=95 PRIMER_PAIR_4_PENALTY=1.298512 PRIMER_LEFT_4_PENALTY=1.067229 PRIMER_RIGHT_4_PENALTY=0.231283 PRIMER_INTERNAL_4_PENALTY=0.494811 PRIMER_LEFT_4_SEQUENCE=GTTCAGTCATTCGGGATGTG PRIMER_RIGHT_4_SEQUENCE=GATGAGAGGACCAGCGTGAT PRIMER_INTERNAL_4_SEQUENCE=GATTTCCACTCCAGGTCCTG PRIMER_LEFT_4=115,20 PRIMER_RIGHT_4=210,20 PRIMER_INTERNAL_4=150,20 PRIMER_LEFT_4_TM=58.933 PRIMER_RIGHT_4_TM=60.231 PRIMER_INTERNAL_4_TM=59.505 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=7.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=6.3000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=96 = SEQUENCE_ID=MH754 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATGATATGGAGAAAAACATTATCTTTCAAAGCTGAAACTTAAGCCTGTGTATTAAAATTNCTTTTCACAACTATTGATAAGACTACCTGGAAAGGAAAGTTGCCTCTATAAGAAATACTAACTGTATCATTGTAGTCAACAGTGAAAGTGAAAAAGAAAGANTTTTTGGAGAAAGCATTAAAAAGTGAAGAGAACAAAGTGTNGCAGACATTTCAACCCAAAATAACATAATTTCCAACGGNGGGTGAA SEQUENCE_INCLUDED_REGION=35,253 P3_COMMENT=CL is TCTTTCAAAGCTGAAACTTAAGC P3_COMMENT=CR is TTTTGGGTTGAAATGTCTGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.139276 PRIMER_LEFT_0_PENALTY=1.091240 PRIMER_RIGHT_0_PENALTY=5.048037 PRIMER_INTERNAL_0_PENALTY=5.848686 PRIMER_LEFT_0_SEQUENCE=TGGAAAGGAAAGTTGCCTCT PRIMER_RIGHT_0_SEQUENCE=CACTTTTTAATGCTTTCTCCAAAAA PRIMER_INTERNAL_0_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA PRIMER_LEFT_0=126,20 PRIMER_RIGHT_0=225,25 PRIMER_INTERNAL_0=174,24 PRIMER_LEFT_0_TM=58.909 PRIMER_RIGHT_0_TM=60.048 PRIMER_INTERNAL_0_TM=58.151 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=28.000 PRIMER_INTERNAL_0_GC_PERCENT=29.167 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=7.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=6.154243 PRIMER_LEFT_1_PENALTY=1.091240 PRIMER_RIGHT_1_PENALTY=5.063004 PRIMER_INTERNAL_1_PENALTY=5.848686 PRIMER_LEFT_1_SEQUENCE=TGGAAAGGAAAGTTGCCTCT PRIMER_RIGHT_1_SEQUENCE=CACTTTTTAATGCTTTCTCCAAAA PRIMER_INTERNAL_1_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA PRIMER_LEFT_1=126,20 PRIMER_RIGHT_1=225,24 PRIMER_INTERNAL_1=174,24 PRIMER_LEFT_1_TM=58.909 PRIMER_RIGHT_1_TM=58.937 PRIMER_INTERNAL_1_TM=58.151 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=29.167 PRIMER_INTERNAL_1_GC_PERCENT=29.167 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=7.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=6.371422 PRIMER_LEFT_2_PENALTY=1.091240 PRIMER_RIGHT_2_PENALTY=5.280182 PRIMER_INTERNAL_2_PENALTY=5.848686 PRIMER_LEFT_2_SEQUENCE=TGGAAAGGAAAGTTGCCTCT PRIMER_RIGHT_2_SEQUENCE=CACTTTTTAATGCTTTCTCCAAA PRIMER_INTERNAL_2_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA PRIMER_LEFT_2=126,20 PRIMER_RIGHT_2=225,23 PRIMER_INTERNAL_2=174,24 PRIMER_LEFT_2_TM=58.909 PRIMER_RIGHT_2_TM=57.720 PRIMER_INTERNAL_2_TM=58.151 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=30.435 PRIMER_INTERNAL_2_GC_PERCENT=29.167 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=7.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=8.8000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=6.599702 PRIMER_LEFT_3_PENALTY=0.600817 PRIMER_RIGHT_3_PENALTY=5.998885 PRIMER_INTERNAL_3_PENALTY=5.848686 PRIMER_LEFT_3_SEQUENCE=CCTGGAAAGGAAAGTTGCCT PRIMER_RIGHT_3_SEQUENCE=CTTTTTAATGCTTTCTCCAAAAA PRIMER_INTERNAL_3_SEQUENCE=TCAACAGTGAAAGTGAAAAAGAAA PRIMER_LEFT_3=124,20 PRIMER_RIGHT_3=223,23 PRIMER_INTERNAL_3=174,24 PRIMER_LEFT_3_TM=60.601 PRIMER_RIGHT_3_TM=57.001 PRIMER_INTERNAL_3_TM=58.151 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=26.087 PRIMER_INTERNAL_3_GC_PERCENT=29.167 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=7.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=6.634142 PRIMER_LEFT_4_PENALTY=6.158436 PRIMER_RIGHT_4_PENALTY=0.475706 PRIMER_INTERNAL_4_PENALTY=8.892047 PRIMER_LEFT_4_SEQUENCE=GGAGAAAAACATTATCTTTCAAAGC PRIMER_RIGHT_4_SEQUENCE=GGCAACTTTCCTTTCCAGGT PRIMER_INTERNAL_4_SEQUENCE=TGAAACTTAAGCCTGTGTATTAAAATT PRIMER_LEFT_4=46,25 PRIMER_RIGHT_4=142,20 PRIMER_INTERNAL_4=71,27 PRIMER_LEFT_4_TM=58.842 PRIMER_RIGHT_4_TM=60.476 PRIMER_INTERNAL_4_TM=58.108 PRIMER_LEFT_4_GC_PERCENT=32.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=25.926 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=97 = SEQUENCE_ID=MH757 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTGATAGGTNTTTAAATTAGAAAGTTACCTGTATTAGCCATATTTACATTTCTGTAGGCTGTACACTCTAAGAATTCTAGTTTAAAAGTCACTGGTTCATTTCAGTATGCATAGCCAGTCTCAAGTTGTCACATTTTGTTCATCAGTTTTCCTTATCTCTTGCATTTTTCAAAATTCTGTGACTTTCAAGCACATTGAACCTTCTTACTTTTACANCTTCACTATCATTAATTATTTTACTTATTATGTTAACACTGCATTATCTGC SEQUENCE_INCLUDED_REGION=35,113 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH760 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATGGGATATTATCATTCAGAGTNNTCCTGTGCCAACGNAGTCTGGGCGCTGGGAACATCTGGAGTCAACATTCTGGCCCATCACAACTCCTTAGGGACCTCCATTCCTCAGGGAATGCACTCAGATCTCAGGCACCAAAACCAACATTGTCTGCTCTTCCTTCCTTTTCTGTCTGGACTTTGTTGGCTGTTGAAAAAAGGAATGAGATTTTCCAAAAGTTTCTTCACAAACTGCTAATGCCAGTACAGGCCAGTTCCATGATTTTACCAGTTCAATCCAGTGATATAG SEQUENCE_INCLUDED_REGION=36,267 P3_COMMENT=CL is CATCTGGAGTCAACATTCTGG P3_COMMENT=CR is AATCATGGAACTGGCCTGTA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.086626 PRIMER_LEFT_0_PENALTY=0.066262 PRIMER_RIGHT_0_PENALTY=0.020364 PRIMER_INTERNAL_0_PENALTY=0.245123 PRIMER_LEFT_0_SEQUENCE=AGTCAACATTCTGGCCCATC PRIMER_RIGHT_0_SEQUENCE=TGGTGCCTGAGATCTGAGTG PRIMER_INTERNAL_0_SEQUENCE=ACCTCCATTCCTCAGGGAAT PRIMER_LEFT_0=101,20 PRIMER_RIGHT_0=175,20 PRIMER_INTERNAL_0=135,20 PRIMER_LEFT_0_TM=59.934 PRIMER_RIGHT_0_TM=59.980 PRIMER_INTERNAL_0_TM=59.755 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=7.00 PRIMER_LEFT_0_END_STABILITY=8.1000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=0.086626 PRIMER_LEFT_1_PENALTY=0.066262 PRIMER_RIGHT_1_PENALTY=0.020364 PRIMER_INTERNAL_1_PENALTY=0.245123 PRIMER_LEFT_1_SEQUENCE=GAGTCAACATTCTGGCCCAT PRIMER_RIGHT_1_SEQUENCE=TGGTGCCTGAGATCTGAGTG PRIMER_INTERNAL_1_SEQUENCE=ACCTCCATTCCTCAGGGAAT PRIMER_LEFT_1=100,20 PRIMER_RIGHT_1=175,20 PRIMER_INTERNAL_1=135,20 PRIMER_LEFT_1_TM=59.934 PRIMER_RIGHT_1_TM=59.980 PRIMER_INTERNAL_1_TM=59.755 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=7.00 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=0.137642 PRIMER_LEFT_2_PENALTY=0.127097 PRIMER_RIGHT_2_PENALTY=0.010545 PRIMER_INTERNAL_2_PENALTY=3.188449 PRIMER_LEFT_2_SEQUENCE=TGTCTGGACTTTGTTGGCTG PRIMER_RIGHT_2_SEQUENCE=ATGGAACTGGCCTGTACTGG PRIMER_INTERNAL_2_SEQUENCE=CCAAAAGTTTCTTCACAAACTGC PRIMER_LEFT_2=208,20 PRIMER_RIGHT_2=297,20 PRIMER_INTERNAL_2=250,23 PRIMER_LEFT_2_TM=59.873 PRIMER_RIGHT_2_TM=59.989 PRIMER_INTERNAL_2_TM=60.188 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=0.200048 PRIMER_LEFT_3_PENALTY=0.072951 PRIMER_RIGHT_3_PENALTY=0.127097 PRIMER_INTERNAL_3_PENALTY=0.495173 PRIMER_LEFT_3_SEQUENCE=CATTCCTCAGGGAATGCACT PRIMER_RIGHT_3_SEQUENCE=CAGCCAACAAAGTCCAGACA PRIMER_INTERNAL_3_SEQUENCE=ATCTCAGGCACCAAAACCAA PRIMER_LEFT_3=140,20 PRIMER_RIGHT_3=227,20 PRIMER_INTERNAL_3=163,20 PRIMER_LEFT_3_TM=60.073 PRIMER_RIGHT_3_TM=59.873 PRIMER_INTERNAL_3_TM=60.495 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=9.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=88 PRIMER_PAIR_4_PENALTY=0.268608 PRIMER_LEFT_4_PENALTY=0.127097 PRIMER_RIGHT_4_PENALTY=0.141511 PRIMER_INTERNAL_4_PENALTY=3.188449 PRIMER_LEFT_4_SEQUENCE=TGTCTGGACTTTGTTGGCTG PRIMER_RIGHT_4_SEQUENCE=GAACTGGCCTGTACTGGCAT PRIMER_INTERNAL_4_SEQUENCE=CCAAAAGTTTCTTCACAAACTGC PRIMER_LEFT_4=208,20 PRIMER_RIGHT_4=294,20 PRIMER_INTERNAL_4=250,23 PRIMER_LEFT_4_TM=59.873 PRIMER_RIGHT_4_TM=60.142 PRIMER_INTERNAL_4_TM=60.188 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=9.6000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH761 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGCTTTAGAGANTCTCACAAGCAAAGTNTAAAATGTTTCCTATCTGGATGCTTGTGATAAATGCAGATTCCTGGGTCTCATCCCAGACTTTCAGAATCTGAATGCCTGAGATATGGCCCAGGAGAGTGCATTAAAACAAAAACAATAACANTACTCCTCCAGGTGCATCTTAAGCCTATCAGAGATTTAATGTGAGACATAGAATGTGAGAATCTTTGTTCTTGATTCCTGAGGTTATATTTTATTCAAACATAAACCCCGNTACACAACGTCCAGCATGCAGTGGG SEQUENCE_INCLUDED_REGION=36,288 P3_COMMENT=CL is TAAAATGTTTCCTATCTGGATGC P3_COMMENT=CR is ATATAACCTCAGGAATCAAGAACA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.835978 PRIMER_LEFT_0_PENALTY=0.622472 PRIMER_RIGHT_0_PENALTY=0.213506 PRIMER_INTERNAL_0_PENALTY=2.044832 PRIMER_LEFT_0_SEQUENCE=ATGCAGATTCCTGGGTCTCA PRIMER_RIGHT_0_SEQUENCE=TTTAATGCACTCTCCTGGGC PRIMER_INTERNAL_0_SEQUENCE=TTCAGAATCTGAATGCCTGAGA PRIMER_LEFT_0=97,20 PRIMER_RIGHT_0=171,20 PRIMER_INTERNAL_0=126,22 PRIMER_LEFT_0_TM=60.622 PRIMER_RIGHT_0_TM=60.214 PRIMER_INTERNAL_0_TM=59.955 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=11.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=11.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=1.119764 PRIMER_LEFT_1_PENALTY=0.906258 PRIMER_RIGHT_1_PENALTY=0.213506 PRIMER_INTERNAL_1_PENALTY=2.044832 PRIMER_LEFT_1_SEQUENCE=AATGCAGATTCCTGGGTCTC PRIMER_RIGHT_1_SEQUENCE=TTTAATGCACTCTCCTGGGC PRIMER_INTERNAL_1_SEQUENCE=TTCAGAATCTGAATGCCTGAGA PRIMER_LEFT_1=96,20 PRIMER_RIGHT_1=171,20 PRIMER_INTERNAL_1=126,22 PRIMER_LEFT_1_TM=59.094 PRIMER_RIGHT_1_TM=60.214 PRIMER_INTERNAL_1_TM=59.955 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=11.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.1000 PRIMER_RIGHT_1_END_STABILITY=11.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=1.136983 PRIMER_LEFT_2_PENALTY=0.973324 PRIMER_RIGHT_2_PENALTY=0.163658 PRIMER_INTERNAL_2_PENALTY=4.285317 PRIMER_LEFT_2_SEQUENCE=TGAATGCCTGAGATATGGCC PRIMER_RIGHT_2_SEQUENCE=GCTTAAGATGCACCTGGAGG PRIMER_INTERNAL_2_SEQUENCE=CAGGAGAGTGCATTAAAACAAAAA PRIMER_LEFT_2=135,20 PRIMER_RIGHT_2=211,20 PRIMER_INTERNAL_2=155,24 PRIMER_LEFT_2_TM=60.973 PRIMER_RIGHT_2_TM=59.836 PRIMER_INTERNAL_2_TM=59.715 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=33.333 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=11.2000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=77 PRIMER_PAIR_3_PENALTY=1.136983 PRIMER_LEFT_3_PENALTY=0.973324 PRIMER_RIGHT_3_PENALTY=0.163658 PRIMER_INTERNAL_3_PENALTY=4.285317 PRIMER_LEFT_3_SEQUENCE=TGAATGCCTGAGATATGGCC PRIMER_RIGHT_3_SEQUENCE=GGCTTAAGATGCACCTGGAG PRIMER_INTERNAL_3_SEQUENCE=CAGGAGAGTGCATTAAAACAAAAA PRIMER_LEFT_3=135,20 PRIMER_RIGHT_3=212,20 PRIMER_INTERNAL_3=155,24 PRIMER_LEFT_3_TM=60.973 PRIMER_RIGHT_3_TM=59.836 PRIMER_INTERNAL_3_TM=59.715 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=33.333 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=11.2000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=78 PRIMER_PAIR_4_PENALTY=1.136983 PRIMER_LEFT_4_PENALTY=0.973324 PRIMER_RIGHT_4_PENALTY=0.163658 PRIMER_INTERNAL_4_PENALTY=4.285317 PRIMER_LEFT_4_SEQUENCE=TGAATGCCTGAGATATGGCC PRIMER_RIGHT_4_SEQUENCE=AGGCTTAAGATGCACCTGGA PRIMER_INTERNAL_4_SEQUENCE=CAGGAGAGTGCATTAAAACAAAAA PRIMER_LEFT_4=135,20 PRIMER_RIGHT_4=213,20 PRIMER_INTERNAL_4=155,24 PRIMER_LEFT_4_TM=60.973 PRIMER_RIGHT_4_TM=59.836 PRIMER_INTERNAL_4_TM=59.715 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=11.2000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH764 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAAAAAAGAGTNNAGTTTCAATACAAAAGGTTAAAAGAAAGAAAGGTCAGAGAAGCAAGTACGATACAGCCAAAGGGCCAGGCAAAATGAAGGTAAAACATAGAGACAAAATCGAGAATCAAAGTACGTTTGAGCATAGGAATGTCCTGATGAAAGTGGCACTTACTGAATATGAATATTGAGGCTAGTTTCAAGGGGACACACATAAGGCCAAGAAGTCAGATGGGAGGATGTCATAATAATCTACATATAATATGGTA SEQUENCE_INCLUDED_REGION=35,261 P3_COMMENT=CL is AAAGAAAGAAAGGTCAGAGAAGC P3_COMMENT=CR is TGACATCCTCCCATCTGACT P3_COMMENT=CM is AGTACGATACAGCCAAAGGGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.392166 PRIMER_LEFT_0_PENALTY=0.074231 PRIMER_RIGHT_0_PENALTY=0.317935 PRIMER_INTERNAL_0_PENALTY=3.301915 PRIMER_LEFT_0_SEQUENCE=GCCAGGCAAAATGAAGGTAA PRIMER_RIGHT_0_SEQUENCE=GTGCCACTTTCATCAGGACA PRIMER_INTERNAL_0_SEQUENCE=CGAGAATCAAAGTACGTTTGAGC PRIMER_LEFT_0=112,20 PRIMER_RIGHT_0=197,20 PRIMER_INTERNAL_0=148,23 PRIMER_LEFT_0_TM=60.074 PRIMER_RIGHT_0_TM=59.682 PRIMER_INTERNAL_0_TM=60.302 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=86 PRIMER_PAIR_1_PENALTY=0.514838 PRIMER_LEFT_1_PENALTY=0.317935 PRIMER_RIGHT_1_PENALTY=0.196903 PRIMER_INTERNAL_1_PENALTY=0.192918 PRIMER_LEFT_1_SEQUENCE=TGTCCTGATGAAAGTGGCAC PRIMER_RIGHT_1_SEQUENCE=CTCCCATCTGACTTCTTGGC PRIMER_INTERNAL_1_SEQUENCE=TGAGGCTAGTTTCAAGGGGA PRIMER_LEFT_1=178,20 PRIMER_RIGHT_1=264,20 PRIMER_INTERNAL_1=215,20 PRIMER_LEFT_1_TM=59.682 PRIMER_RIGHT_1_TM=59.803 PRIMER_INTERNAL_1_TM=59.807 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=87 PRIMER_PAIR_2_PENALTY=0.669082 PRIMER_LEFT_2_PENALTY=0.317935 PRIMER_RIGHT_2_PENALTY=0.351148 PRIMER_INTERNAL_2_PENALTY=0.192918 PRIMER_LEFT_2_SEQUENCE=TGTCCTGATGAAAGTGGCAC PRIMER_RIGHT_2_SEQUENCE=CCTCCCATCTGACTTCTTGG PRIMER_INTERNAL_2_SEQUENCE=TGAGGCTAGTTTCAAGGGGA PRIMER_LEFT_2=178,20 PRIMER_RIGHT_2=265,20 PRIMER_INTERNAL_2=215,20 PRIMER_LEFT_2_TM=59.682 PRIMER_RIGHT_2_TM=59.649 PRIMER_INTERNAL_2_TM=59.807 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=88 PRIMER_PAIR_3_PENALTY=0.984533 PRIMER_LEFT_3_PENALTY=0.074231 PRIMER_RIGHT_3_PENALTY=0.910302 PRIMER_INTERNAL_3_PENALTY=3.301915 PRIMER_LEFT_3_SEQUENCE=GCCAGGCAAAATGAAGGTAA PRIMER_RIGHT_3_SEQUENCE=TGCCACTTTCATCAGGACAT PRIMER_INTERNAL_3_SEQUENCE=CGAGAATCAAAGTACGTTTGAGC PRIMER_LEFT_3=112,20 PRIMER_RIGHT_3=196,20 PRIMER_INTERNAL_3=148,23 PRIMER_LEFT_3_TM=60.074 PRIMER_RIGHT_3_TM=59.090 PRIMER_INTERNAL_3_TM=60.302 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=10.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=6.3000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=85 PRIMER_PAIR_4_PENALTY=1.034061 PRIMER_LEFT_4_PENALTY=0.317935 PRIMER_RIGHT_4_PENALTY=0.716126 PRIMER_INTERNAL_4_PENALTY=0.192918 PRIMER_LEFT_4_SEQUENCE=TGTCCTGATGAAAGTGGCAC PRIMER_RIGHT_4_SEQUENCE=CCATCTGACTTCTTGGCCTT PRIMER_INTERNAL_4_SEQUENCE=TGAGGCTAGTTTCAAGGGGA PRIMER_LEFT_4=178,20 PRIMER_RIGHT_4=261,20 PRIMER_INTERNAL_4=215,20 PRIMER_LEFT_4_TM=59.682 PRIMER_RIGHT_4_TM=59.284 PRIMER_INTERNAL_4_TM=59.807 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=84 = SEQUENCE_ID=MH765 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAATATTTGATCAAGGAGAGAGTCTATGTCCTTATACAGGTTAATTAGACAATCTGATAACAGTCCTATTTGTGACTAGTTACTCTGAGTNCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNNGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT SEQUENCE_INCLUDED_REGION=36,94 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH766 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGTGTNTTTCTCCCTGCCTGGAGCCTGGAGCCTGGGGTCTGGGCCAGGAATTCCCAGGCTAGGGTTGGACTCCAGGTGCAGGAAGCAGCCTGAGGCCCTGTGCAGGTCCAGATCTTCTCAGCCAGCAGGGTATGCCCCCACACAGAGGAGAGAGTNGACTGTGGGTGCCAGGCTGGGGGTGCCTGCAGAGCCTCCAAATCAGTGAACCTCTTCTCTGAGGGTCTCTGTAGGGGGGTGTGAACTTAAACTTGAACTTAAATGAGAGCATCTTAAGCCAGAGGACCAAGTTTTAG SEQUENCE_INCLUDED_REGION=35,164 P3_COMMENT=CL is TTTCTCCCTGCCTGGAGC P3_COMMENT=CR is CTCTCCTCTGTGTGGGGG P3_COMMENT=CM is GGGTCTGGGCCAGGAATT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.978351 PRIMER_LEFT_0_PENALTY=0.431941 PRIMER_RIGHT_0_PENALTY=0.546410 PRIMER_INTERNAL_0_PENALTY=2.388026 PRIMER_LEFT_0_SEQUENCE=CTAGGGTTGGACTCCAGGTG PRIMER_RIGHT_0_SEQUENCE=ATACCCTGCTGGCTGAGAAG PRIMER_INTERNAL_0_SEQUENCE=CCCTGTGCAGGTCCAGAT PRIMER_LEFT_0=95,20 PRIMER_RIGHT_0=169,20 PRIMER_INTERNAL_0=132,18 PRIMER_LEFT_0_TM=59.568 PRIMER_RIGHT_0_TM=59.454 PRIMER_INTERNAL_0_TM=59.612 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=1.057041 PRIMER_LEFT_1_PENALTY=0.510631 PRIMER_RIGHT_1_PENALTY=0.546410 PRIMER_INTERNAL_1_PENALTY=2.388026 PRIMER_LEFT_1_SEQUENCE=GCTAGGGTTGGACTCCAGGT PRIMER_RIGHT_1_SEQUENCE=ATACCCTGCTGGCTGAGAAG PRIMER_INTERNAL_1_SEQUENCE=CCCTGTGCAGGTCCAGAT PRIMER_LEFT_1=94,20 PRIMER_RIGHT_1=169,20 PRIMER_INTERNAL_1=132,18 PRIMER_LEFT_1_TM=60.511 PRIMER_RIGHT_1_TM=59.454 PRIMER_INTERNAL_1_TM=59.612 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=1.113238 PRIMER_LEFT_2_PENALTY=0.816516 PRIMER_RIGHT_2_PENALTY=0.296722 PRIMER_INTERNAL_2_PENALTY=1.972643 PRIMER_LEFT_2_SEQUENCE=GAATTCCCAGGCTAGGGTTG PRIMER_RIGHT_2_SEQUENCE=CCTGCTGGCTGAGAAGATCT PRIMER_INTERNAL_2_SEQUENCE=GACTCCAGGTGCAGGAAGC PRIMER_LEFT_2=84,20 PRIMER_RIGHT_2=165,20 PRIMER_INTERNAL_2=104,19 PRIMER_LEFT_2_TM=60.817 PRIMER_RIGHT_2_TM=59.703 PRIMER_INTERNAL_2_TM=60.973 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=63.158 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.3000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=82 PRIMER_PAIR_3_PENALTY=1.162398 PRIMER_LEFT_3_PENALTY=0.431941 PRIMER_RIGHT_3_PENALTY=0.730458 PRIMER_INTERNAL_3_PENALTY=0.260757 PRIMER_LEFT_3_SEQUENCE=CTAGGGTTGGACTCCAGGTG PRIMER_RIGHT_3_SEQUENCE=ACTCTCTCCTCTGTGTGGGG PRIMER_INTERNAL_3_SEQUENCE=CCTGTGCAGGTCCAGATCTT PRIMER_LEFT_3=95,20 PRIMER_RIGHT_3=191,20 PRIMER_INTERNAL_3=133,20 PRIMER_LEFT_3_TM=59.568 PRIMER_RIGHT_3_TM=59.270 PRIMER_INTERNAL_3_TM=60.261 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=11.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=97 PRIMER_PAIR_4_PENALTY=1.165557 PRIMER_LEFT_4_PENALTY=0.816516 PRIMER_RIGHT_4_PENALTY=0.349041 PRIMER_INTERNAL_4_PENALTY=1.972643 PRIMER_LEFT_4_SEQUENCE=GAATTCCCAGGCTAGGGTTG PRIMER_RIGHT_4_SEQUENCE=TGGCTGAGAAGATCTGGACC PRIMER_INTERNAL_4_SEQUENCE=GACTCCAGGTGCAGGAAGC PRIMER_LEFT_4=84,20 PRIMER_RIGHT_4=160,20 PRIMER_INTERNAL_4=104,19 PRIMER_LEFT_4_TM=60.817 PRIMER_RIGHT_4_TM=60.349 PRIMER_INTERNAL_4_TM=60.973 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=63.158 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=9.1000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=77 = SEQUENCE_ID=MH767 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGGGTGCTGCAGTATCAATCCTCTGAGTNNACTAATATTCCGAGGATTAATACTCAGCCCTCTTGGGNTCNTAGATTTCTGGACCTACTTTGAAATGAGTCTTATACCCATCAAAATATAATGAAGAGGGCATGTCTGTGAAAATATGAAAACCATTCTTATTTTCCATATTAATGAGAAAAGTTAATTTATTTCTCACACTGGGACATTTTAAGTGAAAGGCATACCTTCAATGATACCATATAAATCTTGGATAG SEQUENCE_INCLUDED_REGION=36,258 P3_COMMENT=CL is GGTGCTGCAGTATCAATCCT P3_COMMENT=CR is TGGTATCATTGAAGGTATGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.062077 PRIMER_LEFT_0_PENALTY=2.659966 PRIMER_RIGHT_0_PENALTY=0.402111 PRIMER_INTERNAL_0_PENALTY=5.050278 PRIMER_LEFT_0_SEQUENCE=TTAATACTCAGCCCTCTTGGG PRIMER_RIGHT_0_SEQUENCE=TCACAGACATGCCCTCTTCA PRIMER_INTERNAL_0_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC PRIMER_LEFT_0=83,21 PRIMER_RIGHT_0=177,20 PRIMER_INTERNAL_0=113,25 PRIMER_LEFT_0_TM=58.340 PRIMER_RIGHT_0_TM=60.402 PRIMER_INTERNAL_0_TM=60.050 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=95 PRIMER_PAIR_1_PENALTY=3.618241 PRIMER_LEFT_1_PENALTY=1.274264 PRIMER_RIGHT_1_PENALTY=2.343977 PRIMER_INTERNAL_1_PENALTY=6.803413 PRIMER_LEFT_1_SEQUENCE=AAGAGGGCATGTCTGTGAAAA PRIMER_RIGHT_1_SEQUENCE=GCCTTTCACTTAAAATGTCCCA PRIMER_INTERNAL_1_SEQUENCE=ATGAAAACCATTCTTATTTTCCAT PRIMER_LEFT_1=160,21 PRIMER_RIGHT_1=259,22 PRIMER_INTERNAL_1=182,24 PRIMER_LEFT_1_TM=59.726 PRIMER_RIGHT_1_TM=60.344 PRIMER_INTERNAL_1_TM=57.197 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_INTERNAL_1_GC_PERCENT=25.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=3.729406 PRIMER_LEFT_2_PENALTY=3.327294 PRIMER_RIGHT_2_PENALTY=0.402111 PRIMER_INTERNAL_2_PENALTY=5.050278 PRIMER_LEFT_2_SEQUENCE=GATTAATACTCAGCCCTCTTGGG PRIMER_RIGHT_2_SEQUENCE=TCACAGACATGCCCTCTTCA PRIMER_INTERNAL_2_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC PRIMER_LEFT_2=81,23 PRIMER_RIGHT_2=177,20 PRIMER_INTERNAL_2=113,25 PRIMER_LEFT_2_TM=60.327 PRIMER_RIGHT_2_TM=60.402 PRIMER_INTERNAL_2_TM=60.050 PRIMER_LEFT_2_GC_PERCENT=47.826 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=10.0000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=97 PRIMER_PAIR_3_PENALTY=3.729406 PRIMER_LEFT_3_PENALTY=3.327294 PRIMER_RIGHT_3_PENALTY=0.402111 PRIMER_INTERNAL_3_PENALTY=5.050278 PRIMER_LEFT_3_SEQUENCE=GGATTAATACTCAGCCCTCTTGG PRIMER_RIGHT_3_SEQUENCE=TCACAGACATGCCCTCTTCA PRIMER_INTERNAL_3_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC PRIMER_LEFT_3=80,23 PRIMER_RIGHT_3=177,20 PRIMER_INTERNAL_3=113,25 PRIMER_LEFT_3_TM=60.327 PRIMER_RIGHT_3_TM=60.402 PRIMER_INTERNAL_3_TM=60.050 PRIMER_LEFT_3_GC_PERCENT=47.826 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=3.731007 PRIMER_LEFT_4_PENALTY=3.328895 PRIMER_RIGHT_4_PENALTY=0.402111 PRIMER_INTERNAL_4_PENALTY=5.050278 PRIMER_LEFT_4_SEQUENCE=ATTAATACTCAGCCCTCTTGGG PRIMER_RIGHT_4_SEQUENCE=TCACAGACATGCCCTCTTCA PRIMER_INTERNAL_4_SEQUENCE=TTCTGGACCTACTTTGAAATGAGTC PRIMER_LEFT_4=82,22 PRIMER_RIGHT_4=177,20 PRIMER_INTERNAL_4=113,25 PRIMER_LEFT_4_TM=58.671 PRIMER_RIGHT_4_TM=60.402 PRIMER_INTERNAL_4_TM=60.050 PRIMER_LEFT_4_GC_PERCENT=45.455 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=96 = SEQUENCE_ID=MH771 SEQUENCE_TEMPLATE=GCTTGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTAAGTNGGGAGAAAAAAATTACCTCAAACCCTACCAAGTGTCAAAGCGGCCTTAAAATATTGGTATTTAAAGGAGGTATAATACCAATAAGTCTGAAGTGATTAAGAGGTCTGAGTNAGGACATACAGGGCTGTGTTATGTAGTTTGTGACAGAAATCTTAAATTAAGACGGCAACATAGATGGTGTAACTTACTCGCAGACCTGAAAAAAAAGTGCTGTCAACCCCTCAACTCATGGTGATGGCACTTAGTGAGCATTGGTCTAATAAAGGGGCAAAGAGTGTATGAGTTTCTCCAATTCTGATTTTTTTTTTCTGG SEQUENCE_INCLUDED_REGION=36,192 P3_COMMENT=CL is AGCGGCCTTAAAATATTGGT P3_COMMENT=CR is GTTACACCATCTATGTTGCCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.148265 PRIMER_LEFT_0_PENALTY=0.243575 PRIMER_RIGHT_0_PENALTY=2.904690 PRIMER_INTERNAL_0_PENALTY=6.929653 PRIMER_LEFT_0_SEQUENCE=GTGTCAAAGCGGCCTTAAAA PRIMER_RIGHT_0_SEQUENCE=AACACAGCCCTGTATGTCCT PRIMER_INTERNAL_0_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA PRIMER_LEFT_0=74,20 PRIMER_RIGHT_0=173,20 PRIMER_INTERNAL_0=125,26 PRIMER_LEFT_0_TM=60.244 PRIMER_RIGHT_0_TM=57.095 PRIMER_INTERNAL_0_TM=59.070 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=38.462 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=3.184286 PRIMER_LEFT_1_PENALTY=0.279595 PRIMER_RIGHT_1_PENALTY=2.904690 PRIMER_INTERNAL_1_PENALTY=6.929653 PRIMER_LEFT_1_SEQUENCE=TGTCAAAGCGGCCTTAAAAT PRIMER_RIGHT_1_SEQUENCE=AACACAGCCCTGTATGTCCT PRIMER_INTERNAL_1_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA PRIMER_LEFT_1=75,20 PRIMER_RIGHT_1=173,20 PRIMER_INTERNAL_1=125,26 PRIMER_LEFT_1_TM=59.720 PRIMER_RIGHT_1_TM=57.095 PRIMER_INTERNAL_1_TM=59.070 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=38.462 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=4.059561 PRIMER_LEFT_2_PENALTY=0.279595 PRIMER_RIGHT_2_PENALTY=3.779966 PRIMER_INTERNAL_2_PENALTY=6.929653 PRIMER_LEFT_2_SEQUENCE=TGTCAAAGCGGCCTTAAAAT PRIMER_RIGHT_2_SEQUENCE=TAACACAGCCCTGTATGTCCT PRIMER_INTERNAL_2_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA PRIMER_LEFT_2=75,20 PRIMER_RIGHT_2=174,21 PRIMER_INTERNAL_2=125,26 PRIMER_LEFT_2_TM=59.720 PRIMER_RIGHT_2_TM=57.220 PRIMER_INTERNAL_2_TM=59.070 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=38.462 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=4.108099 PRIMER_LEFT_3_PENALTY=1.490704 PRIMER_RIGHT_3_PENALTY=2.617395 PRIMER_INTERNAL_3_PENALTY=6.929653 PRIMER_LEFT_3_SEQUENCE=AAAGCGGCCTTAAAATATTGG PRIMER_RIGHT_3_SEQUENCE=CATAACACAGCCCTGTATGTCC PRIMER_INTERNAL_3_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA PRIMER_LEFT_3=79,21 PRIMER_RIGHT_3=176,22 PRIMER_INTERNAL_3=125,26 PRIMER_LEFT_3_TM=59.509 PRIMER_RIGHT_3_TM=59.383 PRIMER_INTERNAL_3_TM=59.070 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=38.462 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.4000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=4.164547 PRIMER_LEFT_4_PENALTY=1.259857 PRIMER_RIGHT_4_PENALTY=2.904690 PRIMER_INTERNAL_4_PENALTY=6.929653 PRIMER_LEFT_4_SEQUENCE=TGTCAAAGCGGCCTTAAAATA PRIMER_RIGHT_4_SEQUENCE=AACACAGCCCTGTATGTCCT PRIMER_INTERNAL_4_SEQUENCE=AAGTCTGAAGTGATTAAGAGGTCTGA PRIMER_LEFT_4=75,21 PRIMER_RIGHT_4=173,20 PRIMER_INTERNAL_4=125,26 PRIMER_LEFT_4_TM=59.740 PRIMER_RIGHT_4_TM=57.095 PRIMER_INTERNAL_4_TM=59.070 PRIMER_LEFT_4_GC_PERCENT=38.095 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=38.462 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.2000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = SEQUENCE_ID=MH777 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCCTTCCAGGGAGATGAAGTTATTGAACATCCACTTTAGAAATAGAGCACCATGGCAAGTAGTGCTCTCTGAGGTTCAGGATCCTCTGCAGAGTNNATAATACATCTAGGAAGACATCAGGCTTTAAAAACTCTATGGAGCAACATGTCAACAAATTTGATTTAATATAGGAGAAAAAAAAATCCCCGCATATCTTAGTGCTGAGTGGACACTCTAG SEQUENCE_INCLUDED_REGION=33,217 P3_COMMENT=CL is CCTTCCAGGGAGATGAAGTT P3_COMMENT=CR is ACTCAGCACTAAGATATGCGG P3_COMMENT=CM is TGAACATCCACTTTAGAAATAGAGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.408357 PRIMER_LEFT_0_PENALTY=1.059316 PRIMER_RIGHT_0_PENALTY=0.349041 PRIMER_INTERNAL_0_PENALTY=0.681621 PRIMER_LEFT_0_SEQUENCE=TCCTTCCAGGGAGATGAAGTT PRIMER_RIGHT_0_SEQUENCE=GCAGAGGATCCTGAACCTCA PRIMER_INTERNAL_0_SEQUENCE=CACCATGGCAAGTAGTGCTC PRIMER_LEFT_0=33,21 PRIMER_RIGHT_0=121,20 PRIMER_INTERNAL_0=80,20 PRIMER_LEFT_0_TM=60.059 PRIMER_RIGHT_0_TM=60.349 PRIMER_INTERNAL_0_TM=59.318 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=89 PRIMER_PAIR_1_PENALTY=1.408357 PRIMER_LEFT_1_PENALTY=1.059316 PRIMER_RIGHT_1_PENALTY=0.349041 PRIMER_INTERNAL_1_PENALTY=0.681621 PRIMER_LEFT_1_SEQUENCE=TCCTTCCAGGGAGATGAAGTT PRIMER_RIGHT_1_SEQUENCE=TGCAGAGGATCCTGAACCTC PRIMER_INTERNAL_1_SEQUENCE=CACCATGGCAAGTAGTGCTC PRIMER_LEFT_1=33,21 PRIMER_RIGHT_1=122,20 PRIMER_INTERNAL_1=80,20 PRIMER_LEFT_1_TM=60.059 PRIMER_RIGHT_1_TM=60.349 PRIMER_INTERNAL_1_TM=59.318 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=8.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=90 PRIMER_PAIR_2_PENALTY=1.408357 PRIMER_LEFT_2_PENALTY=1.059316 PRIMER_RIGHT_2_PENALTY=0.349041 PRIMER_INTERNAL_2_PENALTY=0.681621 PRIMER_LEFT_2_SEQUENCE=TCCTTCCAGGGAGATGAAGTT PRIMER_RIGHT_2_SEQUENCE=TCTGCAGAGGATCCTGAACC PRIMER_INTERNAL_2_SEQUENCE=CACCATGGCAAGTAGTGCTC PRIMER_LEFT_2=33,21 PRIMER_RIGHT_2=124,20 PRIMER_INTERNAL_2=80,20 PRIMER_LEFT_2_TM=60.059 PRIMER_RIGHT_2_TM=60.349 PRIMER_INTERNAL_2_TM=59.318 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=92 PRIMER_PAIR_3_PENALTY=1.654525 PRIMER_LEFT_3_PENALTY=1.059316 PRIMER_RIGHT_3_PENALTY=0.595208 PRIMER_INTERNAL_3_PENALTY=0.681621 PRIMER_LEFT_3_SEQUENCE=TCCTTCCAGGGAGATGAAGTT PRIMER_RIGHT_3_SEQUENCE=CTGCAGAGGATCCTGAACCT PRIMER_INTERNAL_3_SEQUENCE=CACCATGGCAAGTAGTGCTC PRIMER_LEFT_3=33,21 PRIMER_RIGHT_3=123,20 PRIMER_INTERNAL_3=80,20 PRIMER_LEFT_3_TM=60.059 PRIMER_RIGHT_3_TM=59.405 PRIMER_INTERNAL_3_TM=59.318 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=91 PRIMER_PAIR_4_PENALTY=1.673877 PRIMER_LEFT_4_PENALTY=0.349041 PRIMER_RIGHT_4_PENALTY=1.324836 PRIMER_INTERNAL_4_PENALTY=4.073155 PRIMER_LEFT_4_SEQUENCE=GGTTCAGGATCCTCTGCAGA PRIMER_RIGHT_4_SEQUENCE=TTTGTTGACATGTTGCTCCA PRIMER_INTERNAL_4_SEQUENCE=CATCTAGGAAGACATCAGGCTTT PRIMER_LEFT_4=105,20 PRIMER_RIGHT_4=187,20 PRIMER_INTERNAL_4=135,23 PRIMER_LEFT_4_TM=60.349 PRIMER_RIGHT_4_TM=58.675 PRIMER_INTERNAL_4_TM=58.927 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=8.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=83 = SEQUENCE_ID=MH779 SEQUENCE_TEMPLATE=CTGAAGGTAAAACTTCTGATTATGTTTGAGAATTTAAATATTAAACGGACACAAATGACATTCTAAATACTTTCTCTTTCAGTTTTGACTTTATTTCTGACCTAATTTTCTATTTGACAAAACTTAGAGTGATTTATGGTGCTAAATCTCATTTAAAAAGAATAAATTCACTAAAGCAAGTTCCATACAAAAATCAGTATCACACATCTTGAGGGCTGTTACATCACTAG SEQUENCE_INCLUDED_REGION=0,230 P3_COMMENT=CL is TGAGAATTTAAATATTAAACGGACA P3_COMMENT=CR is TGATGTAACAGCCCTCAAGA P3_COMMENT=CM is TTCAGTTTTGACTTTATTTCTGACC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.967474 PRIMER_LEFT_0_PENALTY=6.228722 PRIMER_RIGHT_0_PENALTY=0.738752 PRIMER_INTERNAL_0_PENALTY=4.869248 PRIMER_LEFT_0_SEQUENCE=GAGTGATTTATGGTGCTAAATCTCA PRIMER_RIGHT_0_SEQUENCE=ACAGCCCTCAAGATGTGTGA PRIMER_INTERNAL_0_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA PRIMER_LEFT_0=126,25 PRIMER_RIGHT_0=218,20 PRIMER_INTERNAL_0=172,24 PRIMER_LEFT_0_TM=58.771 PRIMER_RIGHT_0_TM=59.261 PRIMER_INTERNAL_0_TM=59.131 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=29.167 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=93 PRIMER_PAIR_1_PENALTY=7.230111 PRIMER_LEFT_1_PENALTY=6.491359 PRIMER_RIGHT_1_PENALTY=0.738752 PRIMER_INTERNAL_1_PENALTY=4.869248 PRIMER_LEFT_1_SEQUENCE=TGGTGCTAAATCTCATTTAAAAAGA PRIMER_RIGHT_1_SEQUENCE=ACAGCCCTCAAGATGTGTGA PRIMER_INTERNAL_1_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA PRIMER_LEFT_1=136,25 PRIMER_RIGHT_1=218,20 PRIMER_INTERNAL_1=172,24 PRIMER_LEFT_1_TM=58.509 PRIMER_RIGHT_1_TM=59.261 PRIMER_INTERNAL_1_TM=59.131 PRIMER_LEFT_1_GC_PERCENT=28.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=29.167 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=7.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=7.494337 PRIMER_LEFT_2_PENALTY=6.755585 PRIMER_RIGHT_2_PENALTY=0.738752 PRIMER_INTERNAL_2_PENALTY=4.869248 PRIMER_LEFT_2_SEQUENCE=TGATTTATGGTGCTAAATCTCATTT PRIMER_RIGHT_2_SEQUENCE=ACAGCCCTCAAGATGTGTGA PRIMER_INTERNAL_2_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA PRIMER_LEFT_2=129,25 PRIMER_RIGHT_2=218,20 PRIMER_INTERNAL_2=172,24 PRIMER_LEFT_2_TM=58.244 PRIMER_RIGHT_2_TM=59.261 PRIMER_INTERNAL_2_TM=59.131 PRIMER_LEFT_2_GC_PERCENT=28.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=29.167 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=7.504078 PRIMER_LEFT_3_PENALTY=6.228722 PRIMER_RIGHT_3_PENALTY=1.275356 PRIMER_INTERNAL_3_PENALTY=4.869248 PRIMER_LEFT_3_SEQUENCE=GAGTGATTTATGGTGCTAAATCTCA PRIMER_RIGHT_3_SEQUENCE=AACAGCCCTCAAGATGTGTG PRIMER_INTERNAL_3_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA PRIMER_LEFT_3=126,25 PRIMER_RIGHT_3=219,20 PRIMER_INTERNAL_3=172,24 PRIMER_LEFT_3_TM=58.771 PRIMER_RIGHT_3_TM=58.725 PRIMER_INTERNAL_3_TM=59.131 PRIMER_LEFT_3_GC_PERCENT=36.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=29.167 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=7.557640 PRIMER_LEFT_4_PENALTY=6.228722 PRIMER_RIGHT_4_PENALTY=1.328918 PRIMER_INTERNAL_4_PENALTY=4.869248 PRIMER_LEFT_4_SEQUENCE=GAGTGATTTATGGTGCTAAATCTCA PRIMER_RIGHT_4_SEQUENCE=CAGCCCTCAAGATGTGTGAT PRIMER_INTERNAL_4_SEQUENCE=AAAGCAAGTTCCATACAAAAATCA PRIMER_LEFT_4=126,25 PRIMER_RIGHT_4=217,20 PRIMER_INTERNAL_4=172,24 PRIMER_LEFT_4_TM=58.771 PRIMER_RIGHT_4_TM=58.671 PRIMER_INTERNAL_4_TM=59.131 PRIMER_LEFT_4_GC_PERCENT=36.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=29.167 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.3000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=92 = SEQUENCE_ID=MH780 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTATAATAAATCTTGAAATCGGGCAGTATAAGCCCTCCCATTTTGTNCTCTTTTTCAAAGTTCTTTGGCTGGTTTATTTCCTCGGTATCTTCATACAAATTCTAGAATTAGATTAGTTCAGCTTTTGGTTGGGGTTGCACTGAATTGATAGATCACTTGTGGAGGTTGGTATCTTGTTAAAGGAAGTGCTTGGAGGGTACGGTCTAACTTTGTTCCTGAATTTAGGGGTAAACATTCAGTTTTCACCCTTGTGTATACTGCTAG SEQUENCE_INCLUDED_REGION=36,196 P3_COMMENT=CL is TGAAATCGGGCAGTATAAGC P3_COMMENT=CR is TCTATCAATTCAGTGCAACCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.376191 PRIMER_LEFT_0_PENALTY=0.067187 PRIMER_RIGHT_0_PENALTY=1.309004 PRIMER_INTERNAL_0_PENALTY=8.521764 PRIMER_LEFT_0_SEQUENCE=TTGGCTGGTTTATTTCCTCG PRIMER_RIGHT_0_SEQUENCE=ATCAATTCAGTGCAACCCCA PRIMER_INTERNAL_0_SEQUENCE=AGAATTAGATTAGTTCAGCTTTTGGT PRIMER_LEFT_0=102,20 PRIMER_RIGHT_0=186,20 PRIMER_INTERNAL_0=141,26 PRIMER_LEFT_0_TM=60.067 PRIMER_RIGHT_0_TM=61.309 PRIMER_INTERNAL_0_TM=57.478 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=30.769 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.9000 PRIMER_RIGHT_0_END_STABILITY=11.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=85 PRIMER_PAIR_1_PENALTY=1.567730 PRIMER_LEFT_1_PENALTY=0.067187 PRIMER_RIGHT_1_PENALTY=1.500543 PRIMER_INTERNAL_1_PENALTY=9.322509 PRIMER_LEFT_1_SEQUENCE=TTGGCTGGTTTATTTCCTCG PRIMER_RIGHT_1_SEQUENCE=CAATTCAGTGCAACCCCAA PRIMER_INTERNAL_1_SEQUENCE=CTAGAATTAGATTAGTTCAGCTTTTGG PRIMER_LEFT_1=102,20 PRIMER_RIGHT_1=184,19 PRIMER_INTERNAL_1=139,27 PRIMER_LEFT_1_TM=60.067 PRIMER_RIGHT_1_TM=60.501 PRIMER_INTERNAL_1_TM=57.677 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=47.368 PRIMER_INTERNAL_1_GC_PERCENT=33.333 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.9000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=1.623955 PRIMER_LEFT_2_PENALTY=0.067187 PRIMER_RIGHT_2_PENALTY=1.556768 PRIMER_INTERNAL_2_PENALTY=8.211922 PRIMER_LEFT_2_SEQUENCE=TTGGCTGGTTTATTTCCTCG PRIMER_RIGHT_2_SEQUENCE=CTATCAATTCAGTGCAACCCC PRIMER_INTERNAL_2_SEQUENCE=GAATTAGATTAGTTCAGCTTTTGGTT PRIMER_LEFT_2=102,20 PRIMER_RIGHT_2=188,21 PRIMER_INTERNAL_2=142,26 PRIMER_LEFT_2_TM=60.067 PRIMER_RIGHT_2_TM=59.443 PRIMER_INTERNAL_2_TM=57.788 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=30.769 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.9000 PRIMER_RIGHT_2_END_STABILITY=10.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=87 PRIMER_PAIR_3_PENALTY=1.628601 PRIMER_LEFT_3_PENALTY=0.319597 PRIMER_RIGHT_3_PENALTY=1.309004 PRIMER_INTERNAL_3_PENALTY=8.521764 PRIMER_LEFT_3_SEQUENCE=GGCTGGTTTATTTCCTCGGT PRIMER_RIGHT_3_SEQUENCE=ATCAATTCAGTGCAACCCCA PRIMER_INTERNAL_3_SEQUENCE=AGAATTAGATTAGTTCAGCTTTTGGT PRIMER_LEFT_3=104,20 PRIMER_RIGHT_3=186,20 PRIMER_INTERNAL_3=141,26 PRIMER_LEFT_3_TM=60.320 PRIMER_RIGHT_3_TM=61.309 PRIMER_INTERNAL_3_TM=57.478 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=30.769 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.6000 PRIMER_RIGHT_3_END_STABILITY=11.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=1.644426 PRIMER_LEFT_4_PENALTY=0.067187 PRIMER_RIGHT_4_PENALTY=1.577239 PRIMER_INTERNAL_4_PENALTY=8.211922 PRIMER_LEFT_4_SEQUENCE=TTGGCTGGTTTATTTCCTCG PRIMER_RIGHT_4_SEQUENCE=TATCAATTCAGTGCAACCCC PRIMER_INTERNAL_4_SEQUENCE=GAATTAGATTAGTTCAGCTTTTGGTT PRIMER_LEFT_4=102,20 PRIMER_RIGHT_4=187,20 PRIMER_INTERNAL_4=142,26 PRIMER_LEFT_4_TM=60.067 PRIMER_RIGHT_4_TM=58.423 PRIMER_INTERNAL_4_TM=57.788 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=30.769 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.9000 PRIMER_RIGHT_4_END_STABILITY=10.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH781 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCTGCTATTTATTAGTAATATAAATGGTCAATGTATGCATTCATTATGCACCAAATAATAACTTCTGTACATACATTATTGTATTTCATTATCACAAAATTATGAGTGAGGGATGATTGTTATCCCTATTTTACAGATGAGAACACTGAGACTTAGAAGAAGTATCTTTCCCAAAGTCACAAAGTTAGTGACAGAGCCGGGATTCGAATCCATCAACTTGAATCCAGAGAAAATGTTCTGCATCACTGTACAACACTGACTCCTTTTTCTCCTTTGAAAACAAGGCAAGATGCA SEQUENCE_INCLUDED_REGION=34,292 P3_COMMENT=CL is TGCATTCATTATGCACCAAA P3_COMMENT=CR is TTGCCTTGTTTTCAAAGGAG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.524661 PRIMER_LEFT_0_PENALTY=1.145399 PRIMER_RIGHT_0_PENALTY=0.379262 PRIMER_INTERNAL_0_PENALTY=2.248900 PRIMER_LEFT_0_SEQUENCE=GGGATTCGAATCCATCAACTT PRIMER_RIGHT_0_SEQUENCE=TGCATCTTGCCTTGTTTTCA PRIMER_INTERNAL_0_SEQUENCE=CCAGAGAAAATGTTCTGCATCA PRIMER_LEFT_0=230,21 PRIMER_RIGHT_0=325,20 PRIMER_INTERNAL_0=255,22 PRIMER_LEFT_0_TM=60.145 PRIMER_RIGHT_0_TM=60.379 PRIMER_INTERNAL_0_TM=60.249 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=96 PRIMER_PAIR_1_PENALTY=1.589777 PRIMER_LEFT_1_PENALTY=1.210515 PRIMER_RIGHT_1_PENALTY=0.379262 PRIMER_INTERNAL_1_PENALTY=2.248900 PRIMER_LEFT_1_SEQUENCE=GGGATTCGAATCCATCAACT PRIMER_RIGHT_1_SEQUENCE=TGCATCTTGCCTTGTTTTCA PRIMER_INTERNAL_1_SEQUENCE=CCAGAGAAAATGTTCTGCATCA PRIMER_LEFT_1=230,20 PRIMER_RIGHT_1=325,20 PRIMER_INTERNAL_1=255,22 PRIMER_LEFT_1_TM=58.789 PRIMER_RIGHT_1_TM=60.379 PRIMER_INTERNAL_1_TM=60.249 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=7.3000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=2.002056 PRIMER_LEFT_2_PENALTY=1.622794 PRIMER_RIGHT_2_PENALTY=0.379262 PRIMER_INTERNAL_2_PENALTY=3.575181 PRIMER_LEFT_2_SEQUENCE=CGAATCCATCAACTTGAATCC PRIMER_RIGHT_2_SEQUENCE=TGCATCTTGCCTTGTTTTCA PRIMER_INTERNAL_2_SEQUENCE=TCTGCATCACTGTACAACACTGA PRIMER_LEFT_2=236,21 PRIMER_RIGHT_2=325,20 PRIMER_INTERNAL_2=268,23 PRIMER_LEFT_2_TM=59.377 PRIMER_RIGHT_2_TM=60.379 PRIMER_INTERNAL_2_TM=59.425 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=7.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=7.3000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=2.002056 PRIMER_LEFT_3_PENALTY=1.622794 PRIMER_RIGHT_3_PENALTY=0.379262 PRIMER_INTERNAL_3_PENALTY=2.248900 PRIMER_LEFT_3_SEQUENCE=GGATTCGAATCCATCAACTTG PRIMER_RIGHT_3_SEQUENCE=TGCATCTTGCCTTGTTTTCA PRIMER_INTERNAL_3_SEQUENCE=CCAGAGAAAATGTTCTGCATCA PRIMER_LEFT_3=231,21 PRIMER_RIGHT_3=325,20 PRIMER_INTERNAL_3=255,22 PRIMER_LEFT_3_TM=59.377 PRIMER_RIGHT_3_TM=60.379 PRIMER_INTERNAL_3_TM=60.249 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=12.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=95 PRIMER_PAIR_4_PENALTY=2.250790 PRIMER_LEFT_4_PENALTY=1.145399 PRIMER_RIGHT_4_PENALTY=1.105391 PRIMER_INTERNAL_4_PENALTY=2.248900 PRIMER_LEFT_4_SEQUENCE=GGGATTCGAATCCATCAACTT PRIMER_RIGHT_4_SEQUENCE=GCATCTTGCCTTGTTTTCAA PRIMER_INTERNAL_4_SEQUENCE=CCAGAGAAAATGTTCTGCATCA PRIMER_LEFT_4=230,21 PRIMER_RIGHT_4=324,20 PRIMER_INTERNAL_4=255,22 PRIMER_LEFT_4_TM=60.145 PRIMER_RIGHT_4_TM=58.895 PRIMER_INTERNAL_4_TM=60.249 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=12.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.3000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=95 = SEQUENCE_ID=MH787 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGGAACCCACTGACTATAAGGCAGCCTCACTCCTAGGAGGAACATCACAGCAACTCGCTAGCAATTGTAAAGTGTAATGATTTTCCCAGTCCTTCCCACCAAAATCTATGCCACTGACTCAGGGGGCTACACACTGAGAAGATGGAAATACCCAGACATTTCAAGGACTACTGGACACAGGCAGGAGTTGAATTTGACACTTGGGGAACCAATGCATCCTCATGGCTCCCCTGCTAAAATGGTAG SEQUENCE_INCLUDED_REGION=36,248 P3_COMMENT=CL is GGAACCCACTGACTATAAGGC P3_COMMENT=CR is CCCCAAGTGTCAAATTCAAC P3_COMMENT=CM is GCCTCACTCCTAGGAGGAAC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.383705 PRIMER_LEFT_0_PENALTY=0.173836 PRIMER_RIGHT_0_PENALTY=0.209870 PRIMER_INTERNAL_0_PENALTY=0.243525 PRIMER_LEFT_0_SEQUENCE=ACTCAGGGGGCTACACACTG PRIMER_RIGHT_0_SEQUENCE=TGGTTCCCCAAGTGTCAAAT PRIMER_INTERNAL_0_SEQUENCE=ACTGGACACAGGCAGGAGTT PRIMER_LEFT_0=155,20 PRIMER_RIGHT_0=249,20 PRIMER_INTERNAL_0=208,20 PRIMER_LEFT_0_TM=60.174 PRIMER_RIGHT_0_TM=60.210 PRIMER_INTERNAL_0_TM=59.756 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=95 PRIMER_PAIR_1_PENALTY=0.383705 PRIMER_LEFT_1_PENALTY=0.173836 PRIMER_RIGHT_1_PENALTY=0.209870 PRIMER_INTERNAL_1_PENALTY=0.243525 PRIMER_LEFT_1_SEQUENCE=ACTCAGGGGGCTACACACTG PRIMER_RIGHT_1_SEQUENCE=ATTGGTTCCCCAAGTGTCAA PRIMER_INTERNAL_1_SEQUENCE=ACTGGACACAGGCAGGAGTT PRIMER_LEFT_1=155,20 PRIMER_RIGHT_1=251,20 PRIMER_INTERNAL_1=208,20 PRIMER_LEFT_1_TM=60.174 PRIMER_RIGHT_1_TM=60.210 PRIMER_INTERNAL_1_TM=59.756 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=97 PRIMER_PAIR_2_PENALTY=0.398177 PRIMER_LEFT_2_PENALTY=0.224341 PRIMER_RIGHT_2_PENALTY=0.173836 PRIMER_INTERNAL_2_PENALTY=0.168650 PRIMER_LEFT_2_SEQUENCE=CAGCAACTCGCTAGCAATTG PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTAGCCCCCTGAGT PRIMER_INTERNAL_2_SEQUENCE=ATTTTCCCAGTCCTTCCCAC PRIMER_LEFT_2=86,20 PRIMER_RIGHT_2=174,20 PRIMER_INTERNAL_2=118,20 PRIMER_LEFT_2_TM=59.776 PRIMER_RIGHT_2_TM=60.174 PRIMER_INTERNAL_2_TM=60.169 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=8.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=89 PRIMER_PAIR_3_PENALTY=0.403485 PRIMER_LEFT_3_PENALTY=0.229650 PRIMER_RIGHT_3_PENALTY=0.173836 PRIMER_INTERNAL_3_PENALTY=0.168650 PRIMER_LEFT_3_SEQUENCE=ATCACAGCAACTCGCTAGCA PRIMER_RIGHT_3_SEQUENCE=CAGTGTGTAGCCCCCTGAGT PRIMER_INTERNAL_3_SEQUENCE=ATTTTCCCAGTCCTTCCCAC PRIMER_LEFT_3=82,20 PRIMER_RIGHT_3=174,20 PRIMER_INTERNAL_3=118,20 PRIMER_LEFT_3_TM=59.770 PRIMER_RIGHT_3_TM=60.174 PRIMER_INTERNAL_3_TM=60.169 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.5000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=93 PRIMER_PAIR_4_PENALTY=0.403485 PRIMER_LEFT_4_PENALTY=0.229650 PRIMER_RIGHT_4_PENALTY=0.173836 PRIMER_INTERNAL_4_PENALTY=0.168650 PRIMER_LEFT_4_SEQUENCE=CATCACAGCAACTCGCTAGC PRIMER_RIGHT_4_SEQUENCE=CAGTGTGTAGCCCCCTGAGT PRIMER_INTERNAL_4_SEQUENCE=ATTTTCCCAGTCCTTCCCAC PRIMER_LEFT_4=81,20 PRIMER_RIGHT_4=174,20 PRIMER_INTERNAL_4=118,20 PRIMER_LEFT_4_TM=59.770 PRIMER_RIGHT_4_TM=60.174 PRIMER_INTERNAL_4_TM=60.169 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=94 = SEQUENCE_ID=MH790 SEQUENCE_TEMPLATE=GGGAAGGTGTGGGGGGCTGCTGGGGCACAGTCATGGGGAACNCCAGTCCCCCTCTCCATGTGCTTCCCTCAATGCTCCCTGATGCCAGCAGTNCTGCCCCCGAACAGAAAGGGGCATTCCTGTGAGTTCCTTGTGTGGGGGACTGGTCACAGAGACTCCCCAAGTNCAGGGCAGGGTGGAGGGAGGCTGAGGGGTGGTTGAATGGACAGGAGAAGAG SEQUENCE_INCLUDED_REGION=0,217 P3_COMMENT=CL is CTGCTGGGGCACAGTCAT P3_COMMENT=CR is TCTTCTCCTGTCCATTCAAC P3_COMMENT=CM is CCAGTCCCCCTCTCCATGT P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.874297 PRIMER_LEFT_0_PENALTY=0.072951 PRIMER_RIGHT_0_PENALTY=0.801345 PRIMER_INTERNAL_0_PENALTY=0.442898 PRIMER_LEFT_0_SEQUENCE=CTCCATGTGCTTCCCTCAAT PRIMER_RIGHT_0_SEQUENCE=AGTCCCCCACACAAGGAACT PRIMER_INTERNAL_0_SEQUENCE=GAACAGAAAGGGGCATTCCT PRIMER_LEFT_0=53,20 PRIMER_RIGHT_0=143,20 PRIMER_INTERNAL_0=101,20 PRIMER_LEFT_0_TM=60.073 PRIMER_RIGHT_0_TM=60.801 PRIMER_INTERNAL_0_TM=60.443 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=91 PRIMER_PAIR_1_PENALTY=0.925391 PRIMER_LEFT_1_PENALTY=0.072951 PRIMER_RIGHT_1_PENALTY=0.852440 PRIMER_INTERNAL_1_PENALTY=2.063598 PRIMER_LEFT_1_SEQUENCE=CTCCATGTGCTTCCCTCAAT PRIMER_RIGHT_1_SEQUENCE=CCACACAAGGAACTCACAGG PRIMER_INTERNAL_1_SEQUENCE=CTCCCTGATGCCAGCAGT PRIMER_LEFT_1=53,20 PRIMER_RIGHT_1=137,20 PRIMER_INTERNAL_1=74,18 PRIMER_LEFT_1_TM=60.073 PRIMER_RIGHT_1_TM=59.148 PRIMER_INTERNAL_1_TM=59.936 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=85 PRIMER_PAIR_2_PENALTY=0.925391 PRIMER_LEFT_2_PENALTY=0.072951 PRIMER_RIGHT_2_PENALTY=0.852440 PRIMER_INTERNAL_2_PENALTY=1.587668 PRIMER_LEFT_2_SEQUENCE=CTCCATGTGCTTCCCTCAAT PRIMER_RIGHT_2_SEQUENCE=CCCACACAAGGAACTCACAG PRIMER_INTERNAL_2_SEQUENCE=CGAACAGAAAGGGGCATTC PRIMER_LEFT_2=53,20 PRIMER_RIGHT_2=138,20 PRIMER_INTERNAL_2=100,19 PRIMER_LEFT_2_TM=60.073 PRIMER_RIGHT_2_TM=59.148 PRIMER_INTERNAL_2_TM=60.588 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=52.632 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=86 PRIMER_PAIR_3_PENALTY=0.932164 PRIMER_LEFT_3_PENALTY=0.072951 PRIMER_RIGHT_3_PENALTY=0.859212 PRIMER_INTERNAL_3_PENALTY=2.063598 PRIMER_LEFT_3_SEQUENCE=CTCCATGTGCTTCCCTCAAT PRIMER_RIGHT_3_SEQUENCE=AAGGAACTCACAGGAATGCC PRIMER_INTERNAL_3_SEQUENCE=CTCCCTGATGCCAGCAGT PRIMER_LEFT_3=53,20 PRIMER_RIGHT_3=131,20 PRIMER_INTERNAL_3=74,18 PRIMER_LEFT_3_TM=60.073 PRIMER_RIGHT_3_TM=59.141 PRIMER_INTERNAL_3_TM=59.936 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=61.111 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=9.6000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=0.955569 PRIMER_LEFT_4_PENALTY=0.072951 PRIMER_RIGHT_4_PENALTY=0.882618 PRIMER_INTERNAL_4_PENALTY=2.063598 PRIMER_LEFT_4_SEQUENCE=CTCCATGTGCTTCCCTCAAT PRIMER_RIGHT_4_SEQUENCE=AACTCACAGGAATGCCCCTT PRIMER_INTERNAL_4_SEQUENCE=CTCCCTGATGCCAGCAGT PRIMER_LEFT_4=53,20 PRIMER_RIGHT_4=127,20 PRIMER_INTERNAL_4=74,18 PRIMER_LEFT_4_TM=60.073 PRIMER_RIGHT_4_TM=60.883 PRIMER_INTERNAL_4_TM=59.936 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH795 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTCAGGGNCAACCAGAAATTCATAGCACAGGAATGGCCGGCAGCAAGGGGTCTGAGAATGTCAAAACAAGCATCCTGGAGGGAAGGCAGATGCCATCAGACTTCTCCAAAGACTCTTTGTACAGACAGTATTTCAGACCATCCCAGGCATCAACAGGAAGTGAGTAGTTACCTTCTGATTGCACGTATAAGGTGACTGGAATACAAAATGCTGGGTTTTTGGCCCGAGGNCCCATGAAGAGATCATAGTTCAAATTTCAACATGTCTAAATTCAGTTCTAGCCACTATGGCTTGCTAGTGTTACCTCAAAATAGAGACATTTATTTTCCCTAA SEQUENCE_INCLUDED_REGION=36,334 P3_COMMENT=CL is ATTCATAGCACAGGAATGGC P3_COMMENT=CR is AGGGAAAATAAATGTCTCTATTTTG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.267394 PRIMER_LEFT_0_PENALTY=0.106310 PRIMER_RIGHT_0_PENALTY=0.161083 PRIMER_INTERNAL_0_PENALTY=1.790576 PRIMER_LEFT_0_SEQUENCE=TATTTCAGACCATCCCAGGC PRIMER_RIGHT_0_SEQUENCE=CCAAAAACCCAGCATTTTGT PRIMER_INTERNAL_0_SEQUENCE=TGCACGTATAAGGTGACTGGA PRIMER_LEFT_0=165,20 PRIMER_RIGHT_0=258,20 PRIMER_INTERNAL_0=216,21 PRIMER_LEFT_0_TM=59.894 PRIMER_RIGHT_0_TM=59.839 PRIMER_INTERNAL_0_TM=59.209 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=94 PRIMER_PAIR_1_PENALTY=0.330555 PRIMER_LEFT_1_PENALTY=0.169471 PRIMER_RIGHT_1_PENALTY=0.161083 PRIMER_INTERNAL_1_PENALTY=1.790576 PRIMER_LEFT_1_SEQUENCE=CAGGCATCAACAGGAAGTGA PRIMER_RIGHT_1_SEQUENCE=CCAAAAACCCAGCATTTTGT PRIMER_INTERNAL_1_SEQUENCE=TGCACGTATAAGGTGACTGGA PRIMER_LEFT_1=180,20 PRIMER_RIGHT_1=258,20 PRIMER_INTERNAL_1=216,21 PRIMER_LEFT_1_TM=59.831 PRIMER_RIGHT_1_TM=59.839 PRIMER_INTERNAL_1_TM=59.209 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=79 PRIMER_PAIR_2_PENALTY=0.335917 PRIMER_LEFT_2_PENALTY=0.166445 PRIMER_RIGHT_2_PENALTY=0.169471 PRIMER_INTERNAL_2_PENALTY=5.792857 PRIMER_LEFT_2_SEQUENCE=AGGCAGATGCCATCAGACTT PRIMER_RIGHT_2_SEQUENCE=TCACTTCCTGTTGATGCCTG PRIMER_INTERNAL_2_SEQUENCE=ACAGACAGTATTTCAGACCATCC PRIMER_LEFT_2=120,20 PRIMER_RIGHT_2=199,20 PRIMER_INTERNAL_2=157,23 PRIMER_LEFT_2_TM=59.834 PRIMER_RIGHT_2_TM=59.831 PRIMER_INTERNAL_2_TM=57.207 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=9.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=7.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=0.415032 PRIMER_LEFT_3_PENALTY=0.308722 PRIMER_RIGHT_3_PENALTY=0.106310 PRIMER_INTERNAL_3_PENALTY=0.166445 PRIMER_LEFT_3_SEQUENCE=TGTCAAAACAAGCATCCTGG PRIMER_RIGHT_3_SEQUENCE=GCCTGGGATGGTCTGAAATA PRIMER_INTERNAL_3_SEQUENCE=AGGCAGATGCCATCAGACTT PRIMER_LEFT_3=95,20 PRIMER_RIGHT_3=184,20 PRIMER_INTERNAL_3=120,20 PRIMER_LEFT_3_TM=59.691 PRIMER_RIGHT_3_TM=59.894 PRIMER_INTERNAL_3_TM=59.834 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=9.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=7.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=0.732726 PRIMER_LEFT_4_PENALTY=0.626415 PRIMER_RIGHT_4_PENALTY=0.106310 PRIMER_INTERNAL_4_PENALTY=0.166445 PRIMER_LEFT_4_SEQUENCE=CAAAACAAGCATCCTGGAGG PRIMER_RIGHT_4_SEQUENCE=GCCTGGGATGGTCTGAAATA PRIMER_INTERNAL_4_SEQUENCE=AGGCAGATGCCATCAGACTT PRIMER_LEFT_4=98,20 PRIMER_RIGHT_4=184,20 PRIMER_INTERNAL_4=120,20 PRIMER_LEFT_4_TM=60.626 PRIMER_RIGHT_4_TM=59.894 PRIMER_INTERNAL_4_TM=59.834 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=9.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=7.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=6.2000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH799 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTGCAGCATTCCATAATGTGAATATATAACACTTTATTCAAAATTTGGGGAAATAGTACCTTGTACATACATAATTTCACATGTTTGCCAGTGTGTGTTTTAGATACATGCCTGGAAGTGAAATTGCAGGTAGCCTGCATATGTTAATTTTGCTAGGTATTACCAAACCAG SEQUENCE_INCLUDED_REGION=36,173 P3_COMMENT=CL is CTGTGCAGCATTCCATAATG P3_COMMENT=CR is AACATATGCAGGCTACCTGC P3_COMMENT=CM is ATAACACTTTATTCAAAATTTGGGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.472484 PRIMER_LEFT_0_PENALTY=1.088344 PRIMER_RIGHT_0_PENALTY=0.384141 PRIMER_INTERNAL_0_PENALTY=3.718759 PRIMER_LEFT_0_SEQUENCE=TGCAGCATTCCATAATGTGAA PRIMER_RIGHT_0_SEQUENCE=CACACACTGGCAAACATGTG PRIMER_INTERNAL_0_SEQUENCE=CACTTTATTCAAAATTTGGGGAA PRIMER_LEFT_0=39,21 PRIMER_RIGHT_0=134,20 PRIMER_INTERNAL_0=67,23 PRIMER_LEFT_0_TM=60.088 PRIMER_RIGHT_0_TM=59.616 PRIMER_INTERNAL_0_TM=59.281 PRIMER_LEFT_0_GC_PERCENT=38.095 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=30.435 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=7.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=96 PRIMER_PAIR_1_PENALTY=1.733889 PRIMER_LEFT_1_PENALTY=1.349748 PRIMER_RIGHT_1_PENALTY=0.384141 PRIMER_INTERNAL_1_PENALTY=3.718759 PRIMER_LEFT_1_SEQUENCE=TGCAGCATTCCATAATGTGA PRIMER_RIGHT_1_SEQUENCE=CACACACTGGCAAACATGTG PRIMER_INTERNAL_1_SEQUENCE=CACTTTATTCAAAATTTGGGGAA PRIMER_LEFT_1=39,20 PRIMER_RIGHT_1=134,20 PRIMER_INTERNAL_1=67,23 PRIMER_LEFT_1_TM=58.650 PRIMER_RIGHT_1_TM=59.616 PRIMER_INTERNAL_1_TM=59.281 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=30.435 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=1.821598 PRIMER_LEFT_2_PENALTY=1.437458 PRIMER_RIGHT_2_PENALTY=0.384141 PRIMER_INTERNAL_2_PENALTY=3.718759 PRIMER_LEFT_2_SEQUENCE=GTGCAGCATTCCATAATGTGA PRIMER_RIGHT_2_SEQUENCE=CACACACTGGCAAACATGTG PRIMER_INTERNAL_2_SEQUENCE=CACTTTATTCAAAATTTGGGGAA PRIMER_LEFT_2=38,21 PRIMER_RIGHT_2=134,20 PRIMER_INTERNAL_2=67,23 PRIMER_LEFT_2_TM=59.563 PRIMER_RIGHT_2_TM=59.616 PRIMER_INTERNAL_2_TM=59.281 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=30.435 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=97 PRIMER_PAIR_3_PENALTY=1.922620 PRIMER_LEFT_3_PENALTY=1.538479 PRIMER_RIGHT_3_PENALTY=0.384141 PRIMER_INTERNAL_3_PENALTY=3.718759 PRIMER_LEFT_3_SEQUENCE=TGTGCAGCATTCCATAATGTG PRIMER_RIGHT_3_SEQUENCE=CACACACTGGCAAACATGTG PRIMER_INTERNAL_3_SEQUENCE=CACTTTATTCAAAATTTGGGGAA PRIMER_LEFT_3=37,21 PRIMER_RIGHT_3=134,20 PRIMER_INTERNAL_3=67,23 PRIMER_LEFT_3_TM=60.538 PRIMER_RIGHT_3_TM=59.616 PRIMER_INTERNAL_3_TM=59.281 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=30.435 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=1.951637 PRIMER_LEFT_4_PENALTY=1.088344 PRIMER_RIGHT_4_PENALTY=0.863293 PRIMER_INTERNAL_4_PENALTY=3.718759 PRIMER_LEFT_4_SEQUENCE=TGCAGCATTCCATAATGTGAA PRIMER_RIGHT_4_SEQUENCE=CACACTGGCAAACATGTGAA PRIMER_INTERNAL_4_SEQUENCE=CACTTTATTCAAAATTTGGGGAA PRIMER_LEFT_4=39,21 PRIMER_RIGHT_4=132,20 PRIMER_INTERNAL_4=67,23 PRIMER_LEFT_4_TM=60.088 PRIMER_RIGHT_4_TM=59.137 PRIMER_INTERNAL_4_TM=59.281 PRIMER_LEFT_4_GC_PERCENT=38.095 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=30.435 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=7.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=94 = SEQUENCE_ID=MH800 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGCTTTTTGTTGTTATTGTCAGTTTGTAACAAACACCATCCTGCTTAGTNTGCACCCTACACTGCTCTAAGCACCTTAATAGGAATGCATTTAATCATTGTAAAAATCCTGGGATTCTATTGCAGGCTTAGGTGACTCAAAAGCCACTCTTTCCACGACAACCCTATTAAAACAAGAAATGTGAATAAGCCATGTTGTTGCTATGCGCTTAGTGAGTCCTCAGTAAATGTTAG SEQUENCE_INCLUDED_REGION=34,234 P3_COMMENT=CL is TTATTGTCAGTTTGTAACAAACACC P3_COMMENT=CR is CTAAGCGCATAGCAACAACA P3_COMMENT=CM is TGCACCCTACACTGCTCTAA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.507626 PRIMER_LEFT_0_PENALTY=0.409420 PRIMER_RIGHT_0_PENALTY=0.098207 PRIMER_INTERNAL_0_PENALTY=0.545130 PRIMER_LEFT_0_SEQUENCE=TGCAGGCTTAGGTGACTCAA PRIMER_RIGHT_0_SEQUENCE=AAGCGCATAGCAACAACATG PRIMER_INTERNAL_0_SEQUENCE=CACTCTTTCCACGACAACCC PRIMER_LEFT_0=155,20 PRIMER_RIGHT_0=244,20 PRIMER_INTERNAL_0=179,20 PRIMER_LEFT_0_TM=59.591 PRIMER_RIGHT_0_TM=59.902 PRIMER_INTERNAL_0_TM=60.545 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=90 PRIMER_PAIR_1_PENALTY=0.507626 PRIMER_LEFT_1_PENALTY=0.409420 PRIMER_RIGHT_1_PENALTY=0.098207 PRIMER_INTERNAL_1_PENALTY=0.545130 PRIMER_LEFT_1_SEQUENCE=TTGCAGGCTTAGGTGACTCA PRIMER_RIGHT_1_SEQUENCE=AAGCGCATAGCAACAACATG PRIMER_INTERNAL_1_SEQUENCE=CACTCTTTCCACGACAACCC PRIMER_LEFT_1=154,20 PRIMER_RIGHT_1=244,20 PRIMER_INTERNAL_1=179,20 PRIMER_LEFT_1_TM=59.591 PRIMER_RIGHT_1_TM=59.902 PRIMER_INTERNAL_1_TM=60.545 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=0.808075 PRIMER_LEFT_2_PENALTY=0.147295 PRIMER_RIGHT_2_PENALTY=0.660780 PRIMER_INTERNAL_2_PENALTY=2.692901 PRIMER_LEFT_2_SEQUENCE=AAAAGCCACTCTTTCCACGA PRIMER_RIGHT_2_SEQUENCE=CTGAGGACTCACTAAGCGCA PRIMER_INTERNAL_2_SEQUENCE=TGAATAAGCCATGTTGTTGCT PRIMER_LEFT_2=173,20 PRIMER_RIGHT_2=257,20 PRIMER_INTERNAL_2=216,21 PRIMER_LEFT_2_TM=59.853 PRIMER_RIGHT_2_TM=59.339 PRIMER_INTERNAL_2_TM=58.307 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=11.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=85 PRIMER_PAIR_3_PENALTY=1.205911 PRIMER_LEFT_3_PENALTY=0.545130 PRIMER_RIGHT_3_PENALTY=0.660780 PRIMER_INTERNAL_3_PENALTY=2.692901 PRIMER_LEFT_3_SEQUENCE=CACTCTTTCCACGACAACCC PRIMER_RIGHT_3_SEQUENCE=CTGAGGACTCACTAAGCGCA PRIMER_INTERNAL_3_SEQUENCE=TGAATAAGCCATGTTGTTGCT PRIMER_LEFT_3=179,20 PRIMER_RIGHT_3=257,20 PRIMER_INTERNAL_3=216,21 PRIMER_LEFT_3_TM=60.545 PRIMER_RIGHT_3_TM=59.339 PRIMER_INTERNAL_3_TM=58.307 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=11.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=1.205911 PRIMER_LEFT_4_PENALTY=0.545130 PRIMER_RIGHT_4_PENALTY=0.660780 PRIMER_INTERNAL_4_PENALTY=2.692901 PRIMER_LEFT_4_SEQUENCE=CCACTCTTTCCACGACAACC PRIMER_RIGHT_4_SEQUENCE=CTGAGGACTCACTAAGCGCA PRIMER_INTERNAL_4_SEQUENCE=TGAATAAGCCATGTTGTTGCT PRIMER_LEFT_4=178,20 PRIMER_RIGHT_4=257,20 PRIMER_INTERNAL_4=216,21 PRIMER_LEFT_4_TM=60.545 PRIMER_RIGHT_4_TM=59.339 PRIMER_INTERNAL_4_TM=58.307 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=11.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH803 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATGCTCCTCAGAGAGNATTTGCATTTGNCTTTTTCAAATCACTGGAGGAATTATTTATATCAGACTACTACAGTTTAATGCCACGCTTTAAACCACACTGGTCATGTGAATATTCTATTTAATTTTACATGATGCTTAAACTGTGCTTTTGAATATTCAAGGTATGTGTGTGTGCGCATGTGTATGTGTATATGTGTGTGTTTACATTAGCCACAAATTAAAGACAGATAAATTTTCTTGCTGACCACTTCTGTGCAATAGG SEQUENCE_INCLUDED_REGION=36,264 P3_COMMENT=CL is ATCAGACTACTACAGTTTAATGCCA P3_COMMENT=CR is ATTGCACAGAAGTGGTCAGC P3_COMMENT=CM is ATTTTACATGATGCTTAAACTGTGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.239865 PRIMER_LEFT_0_PENALTY=0.175676 PRIMER_RIGHT_0_PENALTY=0.064190 PRIMER_INTERNAL_0_PENALTY=3.061184 PRIMER_LEFT_0_SEQUENCE=GCCACGCTTTAAACCACACT PRIMER_RIGHT_0_SEQUENCE=ATGCGCACACACACATACCT PRIMER_INTERNAL_0_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA PRIMER_LEFT_0=117,20 PRIMER_RIGHT_0=216,20 PRIMER_INTERNAL_0=167,23 PRIMER_LEFT_0_TM=60.176 PRIMER_RIGHT_0_TM=60.064 PRIMER_INTERNAL_0_TM=59.939 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.1000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=0.442004 PRIMER_LEFT_1_PENALTY=0.064190 PRIMER_RIGHT_1_PENALTY=0.377815 PRIMER_INTERNAL_1_PENALTY=3.926731 PRIMER_LEFT_1_SEQUENCE=AGGTATGTGTGTGTGCGCAT PRIMER_RIGHT_1_SEQUENCE=GCACAGAAGTGGTCAGCAAG PRIMER_INTERNAL_1_SEQUENCE=TGTGTGTGTTTACATTAGCCACA PRIMER_LEFT_1=197,20 PRIMER_RIGHT_1=293,20 PRIMER_INTERNAL_1=230,23 PRIMER_LEFT_1_TM=60.064 PRIMER_RIGHT_1_TM=59.622 PRIMER_INTERNAL_1_TM=59.073 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=10.1000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=97 PRIMER_PAIR_2_PENALTY=0.823640 PRIMER_LEFT_2_PENALTY=0.759451 PRIMER_RIGHT_2_PENALTY=0.064190 PRIMER_INTERNAL_2_PENALTY=3.061184 PRIMER_LEFT_2_SEQUENCE=CACGCTTTAAACCACACTGG PRIMER_RIGHT_2_SEQUENCE=ATGCGCACACACACATACCT PRIMER_INTERNAL_2_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA PRIMER_LEFT_2=119,20 PRIMER_RIGHT_2=216,20 PRIMER_INTERNAL_2=167,23 PRIMER_LEFT_2_TM=59.241 PRIMER_RIGHT_2_TM=60.064 PRIMER_INTERNAL_2_TM=59.939 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=34.783 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=98 PRIMER_PAIR_3_PENALTY=0.823640 PRIMER_LEFT_3_PENALTY=0.759451 PRIMER_RIGHT_3_PENALTY=0.064190 PRIMER_INTERNAL_3_PENALTY=3.061184 PRIMER_LEFT_3_SEQUENCE=CCACGCTTTAAACCACACTG PRIMER_RIGHT_3_SEQUENCE=ATGCGCACACACACATACCT PRIMER_INTERNAL_3_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA PRIMER_LEFT_3=118,20 PRIMER_RIGHT_3=216,20 PRIMER_INTERNAL_3=167,23 PRIMER_LEFT_3_TM=59.241 PRIMER_RIGHT_3_TM=60.064 PRIMER_INTERNAL_3_TM=59.939 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=34.783 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=0.839027 PRIMER_LEFT_4_PENALTY=0.003166 PRIMER_RIGHT_4_PENALTY=0.835860 PRIMER_INTERNAL_4_PENALTY=3.061184 PRIMER_LEFT_4_SEQUENCE=AATGCCACGCTTTAAACCAC PRIMER_RIGHT_4_SEQUENCE=CGCACACACACATACCTTGA PRIMER_INTERNAL_4_SEQUENCE=TGATGCTTAAACTGTGCTTTTGA PRIMER_LEFT_4=114,20 PRIMER_RIGHT_4=213,20 PRIMER_INTERNAL_4=167,23 PRIMER_LEFT_4_TM=60.003 PRIMER_RIGHT_4_TM=59.164 PRIMER_INTERNAL_4_TM=59.939 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=34.783 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=7.6000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH805 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCACCCAGAGCAAGGAAAGATCATGAAAACCCAAGCAGAAGCAAGACACTGGGTTCCAGCATCAATGCTAAGGGCTAGAACATTTTCTGATGTTTTGCTTGGAAAGGCAGAATTTGGTTGTCTAGTGCGTCCACTAATTGGCCTCCATCTGACACCCTGGAGGGATGGGCTGATTAGAAGCCACAGCTGCTTTGCTCATTATTTCTGATGAATGGACATCACCTGGCGTCTCCGGTGATGGCGCCAG SEQUENCE_INCLUDED_REGION=34,248 P3_COMMENT=CL is AGATCATGAAAACCCAAGCA P3_COMMENT=CR is CAGGTGATGTCCATTCATCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.183953 PRIMER_LEFT_0_PENALTY=0.006945 PRIMER_RIGHT_0_PENALTY=0.177008 PRIMER_INTERNAL_0_PENALTY=0.373535 PRIMER_LEFT_0_SEQUENCE=AAACCCAAGCAGAAGCAAGA PRIMER_RIGHT_0_SEQUENCE=ATTCTGCCTTTCCAAGCAAA PRIMER_INTERNAL_0_SEQUENCE=CAGCATCAATGCTAAGGGCT PRIMER_LEFT_0=61,20 PRIMER_RIGHT_0=147,20 PRIMER_INTERNAL_0=91,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=59.823 PRIMER_INTERNAL_0_TM=60.374 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=87 PRIMER_PAIR_1_PENALTY=0.183953 PRIMER_LEFT_1_PENALTY=0.006945 PRIMER_RIGHT_1_PENALTY=0.177008 PRIMER_INTERNAL_1_PENALTY=0.373535 PRIMER_LEFT_1_SEQUENCE=AAACCCAAGCAGAAGCAAGA PRIMER_RIGHT_1_SEQUENCE=AATTCTGCCTTTCCAAGCAA PRIMER_INTERNAL_1_SEQUENCE=CAGCATCAATGCTAAGGGCT PRIMER_LEFT_1=61,20 PRIMER_RIGHT_1=148,20 PRIMER_INTERNAL_1=91,20 PRIMER_LEFT_1_TM=59.993 PRIMER_RIGHT_1_TM=59.823 PRIMER_INTERNAL_1_TM=60.374 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=88 PRIMER_PAIR_2_PENALTY=0.183953 PRIMER_LEFT_2_PENALTY=0.006945 PRIMER_RIGHT_2_PENALTY=0.177008 PRIMER_INTERNAL_2_PENALTY=0.373535 PRIMER_LEFT_2_SEQUENCE=AAACCCAAGCAGAAGCAAGA PRIMER_RIGHT_2_SEQUENCE=AAATTCTGCCTTTCCAAGCA PRIMER_INTERNAL_2_SEQUENCE=CAGCATCAATGCTAAGGGCT PRIMER_LEFT_2=61,20 PRIMER_RIGHT_2=149,20 PRIMER_INTERNAL_2=91,20 PRIMER_LEFT_2_TM=59.993 PRIMER_RIGHT_2_TM=59.823 PRIMER_INTERNAL_2_TM=60.374 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=89 PRIMER_PAIR_3_PENALTY=0.183953 PRIMER_LEFT_3_PENALTY=0.006945 PRIMER_RIGHT_3_PENALTY=0.177008 PRIMER_INTERNAL_3_PENALTY=0.373535 PRIMER_LEFT_3_SEQUENCE=AAACCCAAGCAGAAGCAAGA PRIMER_RIGHT_3_SEQUENCE=CAAATTCTGCCTTTCCAAGC PRIMER_INTERNAL_3_SEQUENCE=CAGCATCAATGCTAAGGGCT PRIMER_LEFT_3=61,20 PRIMER_RIGHT_3=150,20 PRIMER_INTERNAL_3=91,20 PRIMER_LEFT_3_TM=59.993 PRIMER_RIGHT_3_TM=59.823 PRIMER_INTERNAL_3_TM=60.374 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=0.192383 PRIMER_LEFT_4_PENALTY=0.177008 PRIMER_RIGHT_4_PENALTY=0.015375 PRIMER_INTERNAL_4_PENALTY=0.066262 PRIMER_LEFT_4_SEQUENCE=GCTTGGAAAGGCAGAATTTG PRIMER_RIGHT_4_SEQUENCE=GCTGTGGCTTCTAATCAGCC PRIMER_INTERNAL_4_SEQUENCE=AATTGGCCTCCATCTGACAC PRIMER_LEFT_4=131,20 PRIMER_RIGHT_4=221,20 PRIMER_INTERNAL_4=170,20 PRIMER_LEFT_4_TM=59.823 PRIMER_RIGHT_4_TM=59.985 PRIMER_INTERNAL_4_TM=59.934 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH806 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTAACCTAATATGTACTCTGGGCTGACTTCCATGTTATAACTGTGTGCTTCAATGCTGGCCAAATTTAATCATGCTATAGTTTCCCTCACTTTTCAATTACATTTTTTATTTCCTTACTCTGGACCACCATGACTGCTAGAATTGTCTGCGTGCATTGTCCAAGCCAG SEQUENCE_INCLUDED_REGION=36,171 P3_COMMENT=CL is TGGGCTGACTTCCATGTTAT P3_COMMENT=CR is CAGACAATTCTAGCAGTCATGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.373173 PRIMER_LEFT_0_PENALTY=0.074231 PRIMER_RIGHT_0_PENALTY=0.298942 PRIMER_INTERNAL_0_PENALTY=4.650821 PRIMER_LEFT_0_SEQUENCE=CTTCAATGCTGGCCAAATTT PRIMER_RIGHT_0_SEQUENCE=TAGCAGTCATGGTGGTCCAG PRIMER_INTERNAL_0_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA PRIMER_LEFT_0=86,20 PRIMER_RIGHT_0=177,20 PRIMER_INTERNAL_0=111,24 PRIMER_LEFT_0_TM=60.074 PRIMER_RIGHT_0_TM=59.701 PRIMER_INTERNAL_0_TM=59.349 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=37.500 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=92 PRIMER_PAIR_1_PENALTY=0.373173 PRIMER_LEFT_1_PENALTY=0.074231 PRIMER_RIGHT_1_PENALTY=0.298942 PRIMER_INTERNAL_1_PENALTY=4.650821 PRIMER_LEFT_1_SEQUENCE=CTTCAATGCTGGCCAAATTT PRIMER_RIGHT_1_SEQUENCE=CTAGCAGTCATGGTGGTCCA PRIMER_INTERNAL_1_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA PRIMER_LEFT_1=86,20 PRIMER_RIGHT_1=178,20 PRIMER_INTERNAL_1=111,24 PRIMER_LEFT_1_TM=60.074 PRIMER_RIGHT_1_TM=59.701 PRIMER_INTERNAL_1_TM=59.349 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=93 PRIMER_PAIR_2_PENALTY=0.394429 PRIMER_LEFT_2_PENALTY=0.095488 PRIMER_RIGHT_2_PENALTY=0.298942 PRIMER_INTERNAL_2_PENALTY=4.650821 PRIMER_LEFT_2_SEQUENCE=ACTGTGTGCTTCAATGCTGG PRIMER_RIGHT_2_SEQUENCE=TAGCAGTCATGGTGGTCCAG PRIMER_INTERNAL_2_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA PRIMER_LEFT_2=78,20 PRIMER_RIGHT_2=177,20 PRIMER_INTERNAL_2=111,24 PRIMER_LEFT_2_TM=59.905 PRIMER_RIGHT_2_TM=59.701 PRIMER_INTERNAL_2_TM=59.349 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=1.099900 PRIMER_LEFT_3_PENALTY=0.074231 PRIMER_RIGHT_3_PENALTY=1.025669 PRIMER_INTERNAL_3_PENALTY=4.650821 PRIMER_LEFT_3_SEQUENCE=CTTCAATGCTGGCCAAATTT PRIMER_RIGHT_3_SEQUENCE=CATGGTGGTCCAGAGTAAGGA PRIMER_INTERNAL_3_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA PRIMER_LEFT_3=86,20 PRIMER_RIGHT_3=170,21 PRIMER_INTERNAL_3=111,24 PRIMER_LEFT_3_TM=60.074 PRIMER_RIGHT_3_TM=59.974 PRIMER_INTERNAL_3_TM=59.349 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_INTERNAL_3_GC_PERCENT=37.500 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=85 PRIMER_PAIR_4_PENALTY=1.099900 PRIMER_LEFT_4_PENALTY=0.074231 PRIMER_RIGHT_4_PENALTY=1.025669 PRIMER_INTERNAL_4_PENALTY=4.650821 PRIMER_LEFT_4_SEQUENCE=CTTCAATGCTGGCCAAATTT PRIMER_RIGHT_4_SEQUENCE=TCATGGTGGTCCAGAGTAAGG PRIMER_INTERNAL_4_SEQUENCE=TGCTATAGTTTCCCTCACTTTTCA PRIMER_LEFT_4=86,20 PRIMER_RIGHT_4=171,21 PRIMER_INTERNAL_4=111,24 PRIMER_LEFT_4_TM=60.074 PRIMER_RIGHT_4_TM=59.974 PRIMER_INTERNAL_4_TM=59.349 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=37.500 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=7.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH813 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGTAGCACTTGTTGGGCTAGAGGGTCATGGTGGTNGTNAGGGTAGTTAAATTTTTGTCATGTTTTGAAGTGTGAAGATTTTCCAACCTTCAAAGAATAATAAAGAAGGGAGTGCCCCTCCATAGGTCAAGCAAATGTACAATCACCCAGAGTNATAAAATATGTAGCCTTAGGTGATTGTCAAGTAATTCCCTATATAATTTTTATAAAGATGAGTTTCCCATTGGAACATTTATAAATGACTTATCTATAAAAATTCATTCACCGCACCAATCTTAGG SEQUENCE_INCLUDED_REGION=35,279 P3_COMMENT=CL is ACTTGTTGGGCTAGAGGGTC P3_COMMENT=CR is TGTTCCAATGGGAAACTCAT P3_COMMENT=CM is TAAATTTTTGTCATGTTTTGAAGTG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.659900 PRIMER_LEFT_0_PENALTY=0.246438 PRIMER_RIGHT_0_PENALTY=2.413463 PRIMER_INTERNAL_0_PENALTY=7.279304 PRIMER_LEFT_0_SEQUENCE=CTTGTTGGGCTAGAGGGTCA PRIMER_RIGHT_0_SEQUENCE=TGAAGGTTGGAAAATCTTCACA PRIMER_INTERNAL_0_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG PRIMER_LEFT_0=42,20 PRIMER_RIGHT_0=125,22 PRIMER_INTERNAL_0=74,27 PRIMER_LEFT_0_TM=60.246 PRIMER_RIGHT_0_TM=59.587 PRIMER_INTERNAL_0_TM=60.279 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=36.364 PRIMER_INTERNAL_0_GC_PERCENT=29.630 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=84 PRIMER_PAIR_1_PENALTY=2.863292 PRIMER_LEFT_1_PENALTY=0.449829 PRIMER_RIGHT_1_PENALTY=2.413463 PRIMER_INTERNAL_1_PENALTY=7.279304 PRIMER_LEFT_1_SEQUENCE=TTGTTGGGCTAGAGGGTCAT PRIMER_RIGHT_1_SEQUENCE=TGAAGGTTGGAAAATCTTCACA PRIMER_INTERNAL_1_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG PRIMER_LEFT_1=43,20 PRIMER_RIGHT_1=125,22 PRIMER_INTERNAL_1=74,27 PRIMER_LEFT_1_TM=59.550 PRIMER_RIGHT_1_TM=59.587 PRIMER_INTERNAL_1_TM=60.279 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=36.364 PRIMER_INTERNAL_1_GC_PERCENT=29.630 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.3000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=2.917159 PRIMER_LEFT_2_PENALTY=0.503696 PRIMER_RIGHT_2_PENALTY=2.413463 PRIMER_INTERNAL_2_PENALTY=7.279304 PRIMER_LEFT_2_SEQUENCE=AGCACTTGTTGGGCTAGAGG PRIMER_RIGHT_2_SEQUENCE=TGAAGGTTGGAAAATCTTCACA PRIMER_INTERNAL_2_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG PRIMER_LEFT_2=38,20 PRIMER_RIGHT_2=125,22 PRIMER_INTERNAL_2=74,27 PRIMER_LEFT_2_TM=59.496 PRIMER_RIGHT_2_TM=59.587 PRIMER_INTERNAL_2_TM=60.279 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=36.364 PRIMER_INTERNAL_2_GC_PERCENT=29.630 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=88 PRIMER_PAIR_3_PENALTY=3.205698 PRIMER_LEFT_3_PENALTY=0.792236 PRIMER_RIGHT_3_PENALTY=2.413463 PRIMER_INTERNAL_3_PENALTY=7.279304 PRIMER_LEFT_3_SEQUENCE=CACTTGTTGGGCTAGAGGGT PRIMER_RIGHT_3_SEQUENCE=TGAAGGTTGGAAAATCTTCACA PRIMER_INTERNAL_3_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG PRIMER_LEFT_3=40,20 PRIMER_RIGHT_3=125,22 PRIMER_INTERNAL_3=74,27 PRIMER_LEFT_3_TM=59.208 PRIMER_RIGHT_3_TM=59.587 PRIMER_INTERNAL_3_TM=60.279 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=36.364 PRIMER_INTERNAL_3_GC_PERCENT=29.630 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.1000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=86 PRIMER_PAIR_4_PENALTY=3.468418 PRIMER_LEFT_4_PENALTY=1.054956 PRIMER_RIGHT_4_PENALTY=2.413463 PRIMER_INTERNAL_4_PENALTY=7.279304 PRIMER_LEFT_4_SEQUENCE=TGTTGGGCTAGAGGGTCATG PRIMER_RIGHT_4_SEQUENCE=TGAAGGTTGGAAAATCTTCACA PRIMER_INTERNAL_4_SEQUENCE=GGGTAGTTAAATTTTTGTCATGTTTTG PRIMER_LEFT_4=44,20 PRIMER_RIGHT_4=125,22 PRIMER_INTERNAL_4=74,27 PRIMER_LEFT_4_TM=61.055 PRIMER_RIGHT_4_TM=59.587 PRIMER_INTERNAL_4_TM=60.279 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=36.364 PRIMER_INTERNAL_4_GC_PERCENT=29.630 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH820 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGTTGCTACCCTCGTTCCGATGCTGTCTTTCGCTGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGT SEQUENCE_INCLUDED_REGION=36,33 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH822 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTCTGTATATTCCTGAACTCTGTAGACCTTCCTGACCTGTTTGCAGTGGATACTGTTTTTGAAGGCTCTGTCTCAGTATCTGAAGTTTTTGTCTCCAGAGAAGTCATTTTGTAGGTGTTCCTGGGCGTTTTTGCTACGTTTCCATTTTCTCTAATACACTGCCGTCTTAAGGGAGGGCTTGCAGAGCATTTATCAGATGGCTGTTTTGCTGCATTCTGTGCACTGAAGAACACATTTCCTCCTGAAATAAAAACATACACAATAAAAACATTCTATTAGTGTCTCATGTCAATCA SEQUENCE_INCLUDED_REGION=36,296 P3_COMMENT=CL is CTTCCTGACCTGTTTGCAGT P3_COMMENT=CR is CTTCAGTGCACAGAATGCAG P3_COMMENT=CM is GATACTGTTTTTGAAGGCTCTGTC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.156350 PRIMER_LEFT_0_PENALTY=0.127097 PRIMER_RIGHT_0_PENALTY=0.029253 PRIMER_INTERNAL_0_PENALTY=2.030276 PRIMER_LEFT_0_SEQUENCE=TTCCTGACCTGTTTGCAGTG PRIMER_RIGHT_0_SEQUENCE=GCCCAGGAACACCTACAAAA PRIMER_INTERNAL_0_SEQUENCE=TGTTTTTGAAGGCTCTGTCTCA PRIMER_LEFT_0=65,20 PRIMER_RIGHT_0=162,20 PRIMER_INTERNAL_0=90,22 PRIMER_LEFT_0_TM=59.873 PRIMER_RIGHT_0_TM=59.971 PRIMER_INTERNAL_0_TM=60.030 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=98 PRIMER_PAIR_1_PENALTY=0.216225 PRIMER_LEFT_1_PENALTY=0.029253 PRIMER_RIGHT_1_PENALTY=0.186972 PRIMER_INTERNAL_1_PENALTY=1.621453 PRIMER_LEFT_1_SEQUENCE=TTTTGTAGGTGTTCCTGGGC PRIMER_RIGHT_1_SEQUENCE=GATAAATGCTCTGCAAGCCC PRIMER_INTERNAL_1_SEQUENCE=TACACTGCCGTCTTAAGGGA PRIMER_LEFT_1=143,20 PRIMER_RIGHT_1=230,20 PRIMER_INTERNAL_1=191,20 PRIMER_LEFT_1_TM=59.971 PRIMER_RIGHT_1_TM=59.813 PRIMER_INTERNAL_1_TM=58.379 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=11.2000 PRIMER_RIGHT_1_END_STABILITY=10.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=88 PRIMER_PAIR_2_PENALTY=0.519124 PRIMER_LEFT_2_PENALTY=0.029253 PRIMER_RIGHT_2_PENALTY=0.489871 PRIMER_INTERNAL_2_PENALTY=3.030116 PRIMER_LEFT_2_SEQUENCE=TTTTGTAGGTGTTCCTGGGC PRIMER_RIGHT_2_SEQUENCE=TAAATGCTCTGCAAGCCCTC PRIMER_INTERNAL_2_SEQUENCE=CCATTTTCTCTAATACACTGCCG PRIMER_LEFT_2=143,20 PRIMER_RIGHT_2=228,20 PRIMER_INTERNAL_2=178,23 PRIMER_LEFT_2_TM=59.971 PRIMER_RIGHT_2_TM=60.490 PRIMER_INTERNAL_2_TM=60.030 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=11.2000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=86 PRIMER_PAIR_3_PENALTY=0.851612 PRIMER_LEFT_3_PENALTY=0.822359 PRIMER_RIGHT_3_PENALTY=0.029253 PRIMER_INTERNAL_3_PENALTY=2.030276 PRIMER_LEFT_3_SEQUENCE=CCTTCCTGACCTGTTTGCAG PRIMER_RIGHT_3_SEQUENCE=GCCCAGGAACACCTACAAAA PRIMER_INTERNAL_3_SEQUENCE=TGTTTTTGAAGGCTCTGTCTCA PRIMER_LEFT_3=63,20 PRIMER_RIGHT_3=162,20 PRIMER_INTERNAL_3=90,22 PRIMER_LEFT_3_TM=60.822 PRIMER_RIGHT_3_TM=59.971 PRIMER_INTERNAL_3_TM=60.030 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=1.010289 PRIMER_LEFT_4_PENALTY=0.029253 PRIMER_RIGHT_4_PENALTY=0.981036 PRIMER_INTERNAL_4_PENALTY=0.647368 PRIMER_LEFT_4_SEQUENCE=TTTTGTAGGTGTTCCTGGGC PRIMER_RIGHT_4_SEQUENCE=TGATAAATGCTCTGCAAGCC PRIMER_INTERNAL_4_SEQUENCE=ACACTGCCGTCTTAAGGGAG PRIMER_LEFT_4=143,20 PRIMER_RIGHT_4=231,20 PRIMER_INTERNAL_4=192,20 PRIMER_LEFT_4_TM=59.971 PRIMER_RIGHT_4_TM=59.019 PRIMER_INTERNAL_4_TM=59.353 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=11.2000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH830 SEQUENCE_TEMPLATE=CATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGTTGGGAGAAACAAAAGAGTNNAAAGAGAGACCGAACTCAGAACCGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGTAATAGTCATAAATCAAAATACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCAAAGAAGTCCAAAGATAAAGAAAAATCTAAGTATAGATGAAAGATGAAGAGGCAGAATTGAGAGGCTAACATATTTACTCTTGTCTAACTTAAGAGTGCCAGGAAAGCAGATGCTTAGATTTTGTGTCAAAGCTTGTTATTTTTTTCATACTAGGATTATGGTCTTTAG SEQUENCE_INCLUDED_REGION=31,334 P3_COMMENT=CL is AGAGAGACCGAACTCAGAACC P3_COMMENT=CR is GACCATAATCCTAGTATGAAAAAAA P3_COMMENT=CM is AAGTCGTAGCCGATCTCGAG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.388932 PRIMER_LEFT_0_PENALTY=0.010545 PRIMER_RIGHT_0_PENALTY=1.378387 PRIMER_INTERNAL_0_PENALTY=9.873117 PRIMER_LEFT_0_SEQUENCE=AGACCGAAGCAGAAGTCCAA PRIMER_RIGHT_0_SEQUENCE=GCCTCTCAATTCTGCCTCTT PRIMER_INTERNAL_0_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA PRIMER_LEFT_0=178,20 PRIMER_RIGHT_0=261,20 PRIMER_INTERNAL_0=199,27 PRIMER_LEFT_0_TM=59.989 PRIMER_RIGHT_0_TM=58.622 PRIMER_INTERNAL_0_TM=57.127 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=25.926 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=84 PRIMER_PAIR_1_PENALTY=1.398023 PRIMER_LEFT_1_PENALTY=0.010545 PRIMER_RIGHT_1_PENALTY=1.387477 PRIMER_INTERNAL_1_PENALTY=9.873117 PRIMER_LEFT_1_SEQUENCE=AGACCGAAGCAGAAGTCCAA PRIMER_RIGHT_1_SEQUENCE=AGCCTCTCAATTCTGCCTCTT PRIMER_INTERNAL_1_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA PRIMER_LEFT_1=178,20 PRIMER_RIGHT_1=262,21 PRIMER_INTERNAL_1=199,27 PRIMER_LEFT_1_TM=59.989 PRIMER_RIGHT_1_TM=59.613 PRIMER_INTERNAL_1_TM=57.127 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_INTERNAL_1_GC_PERCENT=25.926 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=85 PRIMER_PAIR_2_PENALTY=1.451173 PRIMER_LEFT_2_PENALTY=0.010545 PRIMER_RIGHT_2_PENALTY=1.440628 PRIMER_INTERNAL_2_PENALTY=9.873117 PRIMER_LEFT_2_SEQUENCE=AGACCGAAGCAGAAGTCCAA PRIMER_RIGHT_2_SEQUENCE=CCTCTCAATTCTGCCTCTTCA PRIMER_INTERNAL_2_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA PRIMER_LEFT_2=178,20 PRIMER_RIGHT_2=260,21 PRIMER_INTERNAL_2=199,27 PRIMER_LEFT_2_TM=59.989 PRIMER_RIGHT_2_TM=59.559 PRIMER_INTERNAL_2_TM=57.127 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=25.926 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=1.488916 PRIMER_LEFT_3_PENALTY=0.010545 PRIMER_RIGHT_3_PENALTY=1.478371 PRIMER_INTERNAL_3_PENALTY=9.873117 PRIMER_LEFT_3_SEQUENCE=AGACCGAAGCAGAAGTCCAA PRIMER_RIGHT_3_SEQUENCE=GCCTCTCAATTCTGCCTCTTC PRIMER_INTERNAL_3_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA PRIMER_LEFT_3=178,20 PRIMER_RIGHT_3=261,21 PRIMER_INTERNAL_3=199,27 PRIMER_LEFT_3_TM=59.989 PRIMER_RIGHT_3_TM=60.478 PRIMER_INTERNAL_3_TM=57.127 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_INTERNAL_3_GC_PERCENT=25.926 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=84 PRIMER_PAIR_4_PENALTY=1.544019 PRIMER_LEFT_4_PENALTY=0.010545 PRIMER_RIGHT_4_PENALTY=1.533474 PRIMER_INTERNAL_4_PENALTY=9.873117 PRIMER_LEFT_4_SEQUENCE=AGACCGAAGCAGAAGTCCAA PRIMER_RIGHT_4_SEQUENCE=TGTTAGCCTCTCAATTCTGCC PRIMER_INTERNAL_4_SEQUENCE=GAAGTCCAAAGATAAAGAAAAATCTAA PRIMER_LEFT_4=178,20 PRIMER_RIGHT_4=266,21 PRIMER_INTERNAL_4=199,27 PRIMER_LEFT_4_TM=59.989 PRIMER_RIGHT_4_TM=59.467 PRIMER_INTERNAL_4_TM=57.127 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_INTERNAL_4_GC_PERCENT=25.926 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH832 SEQUENCE_TEMPLATE=NTTNCNNCAGNTCGACTCTAGAGGATCCCCCTCGTATAAAAGCACCAAGACAGGAATCAAATTGCTCTATTCGGACCCTCATATTAAATAAGAGCAATGAGAGCGAGGGAAAATTGAACTCTCTCAGGTACTGACTGTGGGACCAGACAAGGGATGTAGATTGTCACATTCAATCCTGAAACAAACCTGCCAGGCAAGTCTTCTTCCCATTTTACAAATAAGGAGACAAAAATTAGGAGATTAAATAACTCATCACTGTTTTCAAAATAAGGAGTGTGTGAGGTTTTGTCCCAGGCTCCTGAAGTTTCTGCCTGG SEQUENCE_INCLUDED_REGION=30,285 P3_COMMENT=CL is AAATTGCTCTATTCGGACCC P3_COMMENT=CR is GGGACAAAACCTCACACACT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.428832 PRIMER_LEFT_0_PENALTY=0.295091 PRIMER_RIGHT_0_PENALTY=0.133741 PRIMER_INTERNAL_0_PENALTY=2.975617 PRIMER_LEFT_0_SEQUENCE=TGACTGTGGGACCAGACAAG PRIMER_RIGHT_0_SEQUENCE=GAAGAAGACTTGCCTGGCAG PRIMER_INTERNAL_0_SEQUENCE=TGTCACATTCAATCCTGAAACA PRIMER_LEFT_0=131,20 PRIMER_RIGHT_0=205,20 PRIMER_INTERNAL_0=161,22 PRIMER_LEFT_0_TM=59.705 PRIMER_RIGHT_0_TM=60.134 PRIMER_INTERNAL_0_TM=59.024 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=36.364 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=0.428832 PRIMER_LEFT_1_PENALTY=0.295091 PRIMER_RIGHT_1_PENALTY=0.133741 PRIMER_INTERNAL_1_PENALTY=2.975617 PRIMER_LEFT_1_SEQUENCE=CTGACTGTGGGACCAGACAA PRIMER_RIGHT_1_SEQUENCE=GAAGAAGACTTGCCTGGCAG PRIMER_INTERNAL_1_SEQUENCE=TGTCACATTCAATCCTGAAACA PRIMER_LEFT_1=130,20 PRIMER_RIGHT_1=205,20 PRIMER_INTERNAL_1=161,22 PRIMER_LEFT_1_TM=59.705 PRIMER_RIGHT_1_TM=60.134 PRIMER_INTERNAL_1_TM=59.024 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=36.364 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=0.568163 PRIMER_LEFT_2_PENALTY=0.347318 PRIMER_RIGHT_2_PENALTY=0.220845 PRIMER_INTERNAL_2_PENALTY=0.613219 PRIMER_LEFT_2_SEQUENCE=TGCTCTATTCGGACCCTCAT PRIMER_RIGHT_2_SEQUENCE=TACATCCCTTGTCTGGTCCC PRIMER_INTERNAL_2_SEQUENCE=GAGAGCGAGGGAAAATTGAA PRIMER_LEFT_2=62,20 PRIMER_RIGHT_2=157,20 PRIMER_INTERNAL_2=98,20 PRIMER_LEFT_2_TM=59.653 PRIMER_RIGHT_2_TM=59.779 PRIMER_INTERNAL_2_TM=59.387 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=9.1000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=0.642409 PRIMER_LEFT_3_PENALTY=0.347318 PRIMER_RIGHT_3_PENALTY=0.295091 PRIMER_INTERNAL_3_PENALTY=0.613219 PRIMER_LEFT_3_SEQUENCE=TGCTCTATTCGGACCCTCAT PRIMER_RIGHT_3_SEQUENCE=TTGTCTGGTCCCACAGTCAG PRIMER_INTERNAL_3_SEQUENCE=GAGAGCGAGGGAAAATTGAA PRIMER_LEFT_3=62,20 PRIMER_RIGHT_3=149,20 PRIMER_INTERNAL_3=98,20 PRIMER_LEFT_3_TM=59.653 PRIMER_RIGHT_3_TM=59.705 PRIMER_INTERNAL_3_TM=59.387 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=88 PRIMER_PAIR_4_PENALTY=0.642409 PRIMER_LEFT_4_PENALTY=0.347318 PRIMER_RIGHT_4_PENALTY=0.295091 PRIMER_INTERNAL_4_PENALTY=0.613219 PRIMER_LEFT_4_SEQUENCE=TGCTCTATTCGGACCCTCAT PRIMER_RIGHT_4_SEQUENCE=CTTGTCTGGTCCCACAGTCA PRIMER_INTERNAL_4_SEQUENCE=GAGAGCGAGGGAAAATTGAA PRIMER_LEFT_4=62,20 PRIMER_RIGHT_4=150,20 PRIMER_INTERNAL_4=98,20 PRIMER_LEFT_4_TM=59.653 PRIMER_RIGHT_4_TM=59.705 PRIMER_INTERNAL_4_TM=59.387 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH835 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAATATGCACTGAGCACTAGACAGTGTGCTAAGTACTGAAGATATACACTAAGATAATCCTCACAACCACCGTATGAATTGATGAGGAAACTAAGCTTTAAAGATGTTGATATTTATAAGGTATAGTGTTTCCTGTTCATGTGTTTGAG SEQUENCE_INCLUDED_REGION=34,150 P3_COMMENT=CL is CTAATATGCACTGAGCACTAGACA P3_COMMENT=CR is CACATGAACAGGAAACACTATACC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.968366 PRIMER_LEFT_0_PENALTY=0.040453 PRIMER_RIGHT_0_PENALTY=2.927913 PRIMER_INTERNAL_0_PENALTY=6.882676 PRIMER_LEFT_0_SEQUENCE=TCCTCACAACCACCGTATGA PRIMER_RIGHT_0_SEQUENCE=TCAAACACATGAACAGGAAACA PRIMER_INTERNAL_0_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA PRIMER_LEFT_0=92,20 PRIMER_RIGHT_0=182,22 PRIMER_INTERNAL_0=113,26 PRIMER_LEFT_0_TM=59.960 PRIMER_RIGHT_0_TM=59.072 PRIMER_INTERNAL_0_TM=59.117 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=36.364 PRIMER_INTERNAL_0_GC_PERCENT=30.769 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=91 PRIMER_PAIR_1_PENALTY=3.099994 PRIMER_LEFT_1_PENALTY=0.040453 PRIMER_RIGHT_1_PENALTY=3.059541 PRIMER_INTERNAL_1_PENALTY=6.882676 PRIMER_LEFT_1_SEQUENCE=TCCTCACAACCACCGTATGA PRIMER_RIGHT_1_SEQUENCE=CTCAAACACATGAACAGGAAACA PRIMER_INTERNAL_1_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA PRIMER_LEFT_1=92,20 PRIMER_RIGHT_1=183,23 PRIMER_INTERNAL_1=113,26 PRIMER_LEFT_1_TM=59.960 PRIMER_RIGHT_1_TM=60.060 PRIMER_INTERNAL_1_TM=59.117 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=39.130 PRIMER_INTERNAL_1_GC_PERCENT=30.769 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=5.9000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=92 PRIMER_PAIR_2_PENALTY=3.112338 PRIMER_LEFT_2_PENALTY=0.040453 PRIMER_RIGHT_2_PENALTY=3.071885 PRIMER_INTERNAL_2_PENALTY=6.882676 PRIMER_LEFT_2_SEQUENCE=TCCTCACAACCACCGTATGA PRIMER_RIGHT_2_SEQUENCE=TCAAACACATGAACAGGAAACAC PRIMER_INTERNAL_2_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA PRIMER_LEFT_2=92,20 PRIMER_RIGHT_2=182,23 PRIMER_INTERNAL_2=113,26 PRIMER_LEFT_2_TM=59.960 PRIMER_RIGHT_2_TM=59.928 PRIMER_INTERNAL_2_TM=59.117 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=39.130 PRIMER_INTERNAL_2_GC_PERCENT=30.769 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=5.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=91 PRIMER_PAIR_3_PENALTY=3.513129 PRIMER_LEFT_3_PENALTY=0.585216 PRIMER_RIGHT_3_PENALTY=2.927913 PRIMER_INTERNAL_3_PENALTY=6.882676 PRIMER_LEFT_3_SEQUENCE=CCTCACAACCACCGTATGAA PRIMER_RIGHT_3_SEQUENCE=TCAAACACATGAACAGGAAACA PRIMER_INTERNAL_3_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA PRIMER_LEFT_3=93,20 PRIMER_RIGHT_3=182,22 PRIMER_INTERNAL_3=113,26 PRIMER_LEFT_3_TM=59.415 PRIMER_RIGHT_3_TM=59.072 PRIMER_INTERNAL_3_TM=59.117 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=36.364 PRIMER_INTERNAL_3_GC_PERCENT=30.769 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=3.644757 PRIMER_LEFT_4_PENALTY=0.585216 PRIMER_RIGHT_4_PENALTY=3.059541 PRIMER_INTERNAL_4_PENALTY=6.882676 PRIMER_LEFT_4_SEQUENCE=CCTCACAACCACCGTATGAA PRIMER_RIGHT_4_SEQUENCE=CTCAAACACATGAACAGGAAACA PRIMER_INTERNAL_4_SEQUENCE=TTGATGAGGAAACTAAGCTTTAAAGA PRIMER_LEFT_4=93,20 PRIMER_RIGHT_4=183,23 PRIMER_INTERNAL_4=113,26 PRIMER_LEFT_4_TM=59.415 PRIMER_RIGHT_4_TM=60.060 PRIMER_INTERNAL_4_TM=59.117 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=39.130 PRIMER_INTERNAL_4_GC_PERCENT=30.769 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH839 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGGGATNTNNTGATGATTGTTGAGATTGAAAAAAAATGGCAGTATCATGAAGAGGTNATAATAAATTCTTATTACAATAGTATTAAAAGTGGTTTTTCACTCAGTCAGGGAGAAAAATTAGATTGTACTTTTTGTCCTCTATGATTTCCTTCAGTTATCTTCTAAATGTTGTTTTTTCCCCACAGCCCCCTTAACATTGTTCTCTACGCACTTCTCAATATACACTTTCATTTGTTTCTCAAGCTTCTTTGTGGATGACTCCTAAATATAG SEQUENCE_INCLUDED_REGION=34,274 P3_COMMENT=CL is TTGAGATTGAAAAAAAATGGC P3_COMMENT=CR is TGAAAGTGTATATTGAGAAGTGCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.037056 PRIMER_LEFT_0_PENALTY=1.133436 PRIMER_RIGHT_0_PENALTY=0.903619 PRIMER_INTERNAL_0_PENALTY=4.837064 PRIMER_LEFT_0_SEQUENCE=TGGTTTTTCACTCAGTCAGGG PRIMER_RIGHT_0_SEQUENCE=GCTGTGGGGAAAAAACAACA PRIMER_INTERNAL_0_SEQUENCE=TTTTGTCCTCTATGATTTCCTTCA PRIMER_LEFT_0=126,21 PRIMER_RIGHT_0=222,20 PRIMER_INTERNAL_0=166,24 PRIMER_LEFT_0_TM=60.133 PRIMER_RIGHT_0_TM=60.904 PRIMER_INTERNAL_0_TM=59.163 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=33.333 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=97 PRIMER_PAIR_1_PENALTY=2.154240 PRIMER_LEFT_1_PENALTY=0.147295 PRIMER_RIGHT_1_PENALTY=2.006945 PRIMER_INTERNAL_1_PENALTY=5.472827 PRIMER_LEFT_1_SEQUENCE=CACAGCCCCCTTAACATTGT PRIMER_RIGHT_1_SEQUENCE=TCATCCACAAAGAAGCTTGAGA PRIMER_INTERNAL_1_SEQUENCE=CGCACTTCTCAATATACACTTTCA PRIMER_LEFT_1=217,20 PRIMER_RIGHT_1=294,22 PRIMER_INTERNAL_1=243,24 PRIMER_LEFT_1_TM=59.853 PRIMER_RIGHT_1_TM=59.993 PRIMER_INTERNAL_1_TM=58.527 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_INTERNAL_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=2.390326 PRIMER_LEFT_2_PENALTY=0.147295 PRIMER_RIGHT_2_PENALTY=2.243031 PRIMER_INTERNAL_2_PENALTY=5.472827 PRIMER_LEFT_2_SEQUENCE=CACAGCCCCCTTAACATTGT PRIMER_RIGHT_2_SEQUENCE=GGAGTCATCCACAAAGAAGCTT PRIMER_INTERNAL_2_SEQUENCE=CGCACTTCTCAATATACACTTTCA PRIMER_LEFT_2=217,20 PRIMER_RIGHT_2=298,22 PRIMER_INTERNAL_2=243,24 PRIMER_LEFT_2_TM=59.853 PRIMER_RIGHT_2_TM=59.757 PRIMER_INTERNAL_2_TM=58.527 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_INTERNAL_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=82 PRIMER_PAIR_3_PENALTY=2.577766 PRIMER_LEFT_3_PENALTY=0.570821 PRIMER_RIGHT_3_PENALTY=2.006945 PRIMER_INTERNAL_3_PENALTY=5.472827 PRIMER_LEFT_3_SEQUENCE=CAGCCCCCTTAACATTGTTC PRIMER_RIGHT_3_SEQUENCE=TCATCCACAAAGAAGCTTGAGA PRIMER_INTERNAL_3_SEQUENCE=CGCACTTCTCAATATACACTTTCA PRIMER_LEFT_3=219,20 PRIMER_RIGHT_3=294,22 PRIMER_INTERNAL_3=243,24 PRIMER_LEFT_3_TM=59.429 PRIMER_RIGHT_3_TM=59.993 PRIMER_INTERNAL_3_TM=58.527 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=40.909 PRIMER_INTERNAL_3_GC_PERCENT=37.500 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=2.653445 PRIMER_LEFT_4_PENALTY=0.147295 PRIMER_RIGHT_4_PENALTY=2.506150 PRIMER_INTERNAL_4_PENALTY=5.472827 PRIMER_LEFT_4_SEQUENCE=CACAGCCCCCTTAACATTGT PRIMER_RIGHT_4_SEQUENCE=CATCCACAAAGAAGCTTGAGAA PRIMER_INTERNAL_4_SEQUENCE=CGCACTTCTCAATATACACTTTCA PRIMER_LEFT_4=217,20 PRIMER_RIGHT_4=293,22 PRIMER_INTERNAL_4=243,24 PRIMER_LEFT_4_TM=59.853 PRIMER_RIGHT_4_TM=59.494 PRIMER_INTERNAL_4_TM=58.527 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=40.909 PRIMER_INTERNAL_4_GC_PERCENT=37.500 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=77 = SEQUENCE_ID=MH842 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTATGCCAAAGAATTAAAAACACACAGAATCATCAAAGCACAATCTGTGTTTGAGATAAATGATAGTCTGAGTCACCTATGTAAGAAGTAACTCTGAAATAGTAGGATAGTATTATCATTTCCTGTAATAGATTCACCTCTCAGCAATTGGTCTGTTTTCATTCTATGGAAACTCTCCGTACTGTAATTTTCATTCTATGGAAACTCCCCATACTGTAATTGGACAGTTTTGGTTTCCACACCAG SEQUENCE_INCLUDED_REGION=36,246 P3_COMMENT=CL is AAACACACAGAATCATCAAAGC P3_COMMENT=CR is GTGGAAACCAAAACTGTCCA P3_COMMENT=CM is TCATTTCCTGTAATAGATTCACCTC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.697489 PRIMER_LEFT_0_PENALTY=1.692817 PRIMER_RIGHT_0_PENALTY=1.004672 PRIMER_INTERNAL_0_PENALTY=1.627348 PRIMER_LEFT_0_SEQUENCE=TCAGCAATTGGTCTGTTTTCA PRIMER_RIGHT_0_SEQUENCE=TGGAAACCAAAACTGTCCAA PRIMER_INTERNAL_0_SEQUENCE=TCATTCTATGGAAACTCCCCA PRIMER_LEFT_0=177,21 PRIMER_RIGHT_0=275,20 PRIMER_INTERNAL_0=227,21 PRIMER_LEFT_0_TM=59.307 PRIMER_RIGHT_0_TM=58.995 PRIMER_INTERNAL_0_TM=59.373 PRIMER_LEFT_0_GC_PERCENT=38.095 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=6.00 PRIMER_PAIR_0_PRODUCT_SIZE=99 PRIMER_PAIR_1_PENALTY=2.768302 PRIMER_LEFT_1_PENALTY=1.140954 PRIMER_RIGHT_1_PENALTY=1.627348 PRIMER_INTERNAL_1_PENALTY=3.382873 PRIMER_LEFT_1_SEQUENCE=TCACCTCTCAGCAATTGGTCT PRIMER_RIGHT_1_SEQUENCE=TGGGGAGTTTCCATAGAATGA PRIMER_INTERNAL_1_SEQUENCE=TTTCATTCTATGGAAACTCTCCG PRIMER_LEFT_1=170,21 PRIMER_RIGHT_1=247,21 PRIMER_INTERNAL_1=193,23 PRIMER_LEFT_1_TM=59.859 PRIMER_RIGHT_1_TM=59.373 PRIMER_INTERNAL_1_TM=59.617 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=2.825193 PRIMER_LEFT_2_PENALTY=1.197845 PRIMER_RIGHT_2_PENALTY=1.627348 PRIMER_INTERNAL_2_PENALTY=3.382873 PRIMER_LEFT_2_SEQUENCE=TCACCTCTCAGCAATTGGTC PRIMER_RIGHT_2_SEQUENCE=TGGGGAGTTTCCATAGAATGA PRIMER_INTERNAL_2_SEQUENCE=TTTCATTCTATGGAAACTCTCCG PRIMER_LEFT_2=170,20 PRIMER_RIGHT_2=247,21 PRIMER_INTERNAL_2=193,23 PRIMER_LEFT_2_TM=58.802 PRIMER_RIGHT_2_TM=59.373 PRIMER_INTERNAL_2_TM=59.617 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=78 PRIMER_PAIR_3_PENALTY=2.832009 PRIMER_LEFT_3_PENALTY=1.692817 PRIMER_RIGHT_3_PENALTY=1.139192 PRIMER_INTERNAL_3_PENALTY=1.627348 PRIMER_LEFT_3_SEQUENCE=TCAGCAATTGGTCTGTTTTCA PRIMER_RIGHT_3_SEQUENCE=GTGGAAACCAAAACTGTCCAA PRIMER_INTERNAL_3_SEQUENCE=TCATTCTATGGAAACTCCCCA PRIMER_LEFT_3=177,21 PRIMER_RIGHT_3=276,21 PRIMER_INTERNAL_3=227,21 PRIMER_LEFT_3_TM=59.307 PRIMER_RIGHT_3_TM=59.861 PRIMER_INTERNAL_3_TM=59.373 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_INTERNAL_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=8.00 PRIMER_PAIR_3_COMPL_END=6.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=2.874914 PRIMER_LEFT_4_PENALTY=1.247566 PRIMER_RIGHT_4_PENALTY=1.627348 PRIMER_INTERNAL_4_PENALTY=3.382873 PRIMER_LEFT_4_SEQUENCE=TTCACCTCTCAGCAATTGGTC PRIMER_RIGHT_4_SEQUENCE=TGGGGAGTTTCCATAGAATGA PRIMER_INTERNAL_4_SEQUENCE=TTTCATTCTATGGAAACTCTCCG PRIMER_LEFT_4=169,21 PRIMER_RIGHT_4=247,21 PRIMER_INTERNAL_4=193,23 PRIMER_LEFT_4_TM=60.248 PRIMER_RIGHT_4_TM=59.373 PRIMER_INTERNAL_4_TM=59.617 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH851 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTCTGAGAAGGGGAAAATCTGTCTGCTTAAAGATCTCACTAAACAAGTGCTGTACAGTGACAAGTTACTTATTTTCTAATACCTTTCAGAGGCAGATGACGTATTTTATTTTCCTCTGAGGTCAACAAAAGAAATGGGGTATTATCTGATGATCTACTTTAACATTCTCTATATGAGAAACAAGAGTGTCTAATGTAAAACACAGGATAATTGTGTGTGTGTATGTGTATACAAGCATAAAATGTTTCAGGAAATAATTTCTATAAGTTGATTCTGAAAATAAATCAGTAATAAAACAGTTA SEQUENCE_INCLUDED_REGION=33,304 P3_COMMENT=CL is AAGGGGAAAATCTGTCTGCT P3_COMMENT=CR is AATTATTTCCTGAAACATTTTATGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.216468 PRIMER_LEFT_0_PENALTY=1.282865 PRIMER_RIGHT_0_PENALTY=0.933604 PRIMER_INTERNAL_0_PENALTY=5.432436 PRIMER_LEFT_0_SEQUENCE=GAAGGGGAAAATCTGTCTGC PRIMER_RIGHT_0_SEQUENCE=CGTCATCTGCCTCTGAAAGG PRIMER_INTERNAL_0_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG PRIMER_LEFT_0=41,20 PRIMER_RIGHT_0=135,20 PRIMER_INTERNAL_0=75,24 PRIMER_LEFT_0_TM=58.717 PRIMER_RIGHT_0_TM=60.934 PRIMER_INTERNAL_0_TM=58.568 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=95 PRIMER_PAIR_1_PENALTY=2.229065 PRIMER_LEFT_1_PENALTY=1.295461 PRIMER_RIGHT_1_PENALTY=0.933604 PRIMER_INTERNAL_1_PENALTY=5.432436 PRIMER_LEFT_1_SEQUENCE=GAAGGGGAAAATCTGTCTGCT PRIMER_RIGHT_1_SEQUENCE=CGTCATCTGCCTCTGAAAGG PRIMER_INTERNAL_1_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG PRIMER_LEFT_1=41,21 PRIMER_RIGHT_1=135,20 PRIMER_INTERNAL_1=75,24 PRIMER_LEFT_1_TM=59.705 PRIMER_RIGHT_1_TM=60.934 PRIMER_INTERNAL_1_TM=58.568 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=95 PRIMER_PAIR_2_PENALTY=2.229065 PRIMER_LEFT_2_PENALTY=1.295461 PRIMER_RIGHT_2_PENALTY=0.933604 PRIMER_INTERNAL_2_PENALTY=5.432436 PRIMER_LEFT_2_SEQUENCE=AGAAGGGGAAAATCTGTCTGC PRIMER_RIGHT_2_SEQUENCE=CGTCATCTGCCTCTGAAAGG PRIMER_INTERNAL_2_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG PRIMER_LEFT_2=40,21 PRIMER_RIGHT_2=135,20 PRIMER_INTERNAL_2=75,24 PRIMER_LEFT_2_TM=59.705 PRIMER_RIGHT_2_TM=60.934 PRIMER_INTERNAL_2_TM=58.568 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=2.706794 PRIMER_LEFT_3_PENALTY=1.773191 PRIMER_RIGHT_3_PENALTY=0.933604 PRIMER_INTERNAL_3_PENALTY=5.432436 PRIMER_LEFT_3_SEQUENCE=AAGGGGAAAATCTGTCTGCTT PRIMER_RIGHT_3_SEQUENCE=CGTCATCTGCCTCTGAAAGG PRIMER_INTERNAL_3_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG PRIMER_LEFT_3=42,21 PRIMER_RIGHT_3=135,20 PRIMER_INTERNAL_3=75,24 PRIMER_LEFT_3_TM=59.227 PRIMER_RIGHT_3_TM=60.934 PRIMER_INTERNAL_3_TM=58.568 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=3.080450 PRIMER_LEFT_4_PENALTY=2.146847 PRIMER_RIGHT_4_PENALTY=0.933604 PRIMER_INTERNAL_4_PENALTY=5.432436 PRIMER_LEFT_4_SEQUENCE=AGGGGAAAATCTGTCTGCTT PRIMER_RIGHT_4_SEQUENCE=CGTCATCTGCCTCTGAAAGG PRIMER_INTERNAL_4_SEQUENCE=AAACAAGTGCTGTACAGTGACAAG PRIMER_LEFT_4=43,20 PRIMER_RIGHT_4=135,20 PRIMER_INTERNAL_4=75,24 PRIMER_LEFT_4_TM=57.853 PRIMER_RIGHT_4_TM=60.934 PRIMER_INTERNAL_4_TM=58.568 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=93 = SEQUENCE_ID=MH861 SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGTGAATTTNCCACAGCCCCAGGGAGAACAATTATAGTGACCAACATTTACCTTTGCTGGAGACTTCCTGAATGGGAGCAGGGGCAGATACAGGGACCTACAGGACTGAGACTGCATAGAAGATGCTGAAGAAGACAGATGATTGCCAAGAGTTAGATCCCCCTCCCTACCATCCCTGGAACTTGGATGCAACCCTGAAGAAGATAGGATAAAAAAATGCTGAAGTCAATGAACTCACCTACATTTAGTAAGATTAGTTTCCTTCTGTCGTTTTTGCCTGAGGAG SEQUENCE_INCLUDED_REGION=34,289 P3_COMMENT=CL is CAGGGAGAACAATTATAGTGACC P3_COMMENT=CR is TTGGCAATCATCTGTCTTCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.258372 PRIMER_LEFT_0_PENALTY=0.111077 PRIMER_RIGHT_0_PENALTY=0.147295 PRIMER_INTERNAL_0_PENALTY=2.298898 PRIMER_LEFT_0_SEQUENCE=ACTTGGATGCAACCCTGAAG PRIMER_RIGHT_0_SEQUENCE=AGGCAAAAACGACAGAAGGA PRIMER_INTERNAL_0_SEQUENCE=TGCTGAAGTCAATGAACTCACC PRIMER_LEFT_0=218,20 PRIMER_RIGHT_0=316,20 PRIMER_INTERNAL_0=255,22 PRIMER_LEFT_0_TM=60.111 PRIMER_RIGHT_0_TM=59.853 PRIMER_INTERNAL_0_TM=60.299 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=7.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=99 PRIMER_PAIR_1_PENALTY=0.338433 PRIMER_LEFT_1_PENALTY=0.111077 PRIMER_RIGHT_1_PENALTY=0.227356 PRIMER_INTERNAL_1_PENALTY=2.298898 PRIMER_LEFT_1_SEQUENCE=ACTTGGATGCAACCCTGAAG PRIMER_RIGHT_1_SEQUENCE=GGCAAAAACGACAGAAGGAA PRIMER_INTERNAL_1_SEQUENCE=TGCTGAAGTCAATGAACTCACC PRIMER_LEFT_1=218,20 PRIMER_RIGHT_1=315,20 PRIMER_INTERNAL_1=255,22 PRIMER_LEFT_1_TM=60.111 PRIMER_RIGHT_1_TM=60.227 PRIMER_INTERNAL_1_TM=60.299 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=7.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=98 PRIMER_PAIR_2_PENALTY=0.342653 PRIMER_LEFT_2_PENALTY=0.195358 PRIMER_RIGHT_2_PENALTY=0.147295 PRIMER_INTERNAL_2_PENALTY=2.298898 PRIMER_LEFT_2_SEQUENCE=GGATGCAACCCTGAAGAAGA PRIMER_RIGHT_2_SEQUENCE=AGGCAAAAACGACAGAAGGA PRIMER_INTERNAL_2_SEQUENCE=TGCTGAAGTCAATGAACTCACC PRIMER_LEFT_2=222,20 PRIMER_RIGHT_2=316,20 PRIMER_INTERNAL_2=255,22 PRIMER_LEFT_2_TM=60.195 PRIMER_RIGHT_2_TM=59.853 PRIMER_INTERNAL_2_TM=60.299 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=7.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=95 PRIMER_PAIR_3_PENALTY=0.422714 PRIMER_LEFT_3_PENALTY=0.195358 PRIMER_RIGHT_3_PENALTY=0.227356 PRIMER_INTERNAL_3_PENALTY=2.298898 PRIMER_LEFT_3_SEQUENCE=GGATGCAACCCTGAAGAAGA PRIMER_RIGHT_3_SEQUENCE=GGCAAAAACGACAGAAGGAA PRIMER_INTERNAL_3_SEQUENCE=TGCTGAAGTCAATGAACTCACC PRIMER_LEFT_3=222,20 PRIMER_RIGHT_3=315,20 PRIMER_INTERNAL_3=255,22 PRIMER_LEFT_3_TM=60.195 PRIMER_RIGHT_3_TM=60.227 PRIMER_INTERNAL_3_TM=60.299 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.455 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=7.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=0.642468 PRIMER_LEFT_4_PENALTY=0.495173 PRIMER_RIGHT_4_PENALTY=0.147295 PRIMER_INTERNAL_4_PENALTY=2.298898 PRIMER_LEFT_4_SEQUENCE=AACTTGGATGCAACCCTGAA PRIMER_RIGHT_4_SEQUENCE=AGGCAAAAACGACAGAAGGA PRIMER_INTERNAL_4_SEQUENCE=TGCTGAAGTCAATGAACTCACC PRIMER_LEFT_4=217,20 PRIMER_RIGHT_4=316,20 PRIMER_INTERNAL_4=255,22 PRIMER_LEFT_4_TM=60.495 PRIMER_RIGHT_4_TM=59.853 PRIMER_INTERNAL_4_TM=60.299 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.455 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=7.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH862 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTATTTGTATTGCTGGAAACAATAGGATAATTTGCTGAGGTTGGGGAGTTTCTCCCNTCCAGAGAGTNCTTAATCTTCCAAAATTTGGTTGAGATCCAAGGTTGATTTTGCTCTACGACTTTTTTTCTGAAGTTTTACTCATTTCCAATAAGAAGGCATGTTTTCCTGCTTCCATGATGATGGAGAACAGGCACATCCTTTCTTGAGTTTCAGCGTGCTTCTGACAGGGAGGGTGAGTGTGAGATTTTTCCTTCTTGTAAGATGGTAGAGAGCCATCATCAGCCTGAGCCATATTTCCAAGTA SEQUENCE_INCLUDED_REGION=34,302 P3_COMMENT=CL is TATTTGTATTGCTGGAAACAAT P3_COMMENT=CR is ACTTGGAAATATGGCTCAGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.233904 PRIMER_LEFT_0_PENALTY=0.080962 PRIMER_RIGHT_0_PENALTY=0.152942 PRIMER_INTERNAL_0_PENALTY=0.362999 PRIMER_LEFT_0_SEQUENCE=GCATGTTTTCCTGCTTCCAT PRIMER_RIGHT_0_SEQUENCE=ACACTCACCCTCCCTGTCAG PRIMER_INTERNAL_0_SEQUENCE=TGATGGAGAACAGGCACATC PRIMER_LEFT_0=188,20 PRIMER_RIGHT_0=272,20 PRIMER_INTERNAL_0=210,20 PRIMER_LEFT_0_TM=60.081 PRIMER_RIGHT_0_TM=60.153 PRIMER_INTERNAL_0_TM=59.637 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.1000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=85 PRIMER_PAIR_1_PENALTY=0.233904 PRIMER_LEFT_1_PENALTY=0.080962 PRIMER_RIGHT_1_PENALTY=0.152942 PRIMER_INTERNAL_1_PENALTY=0.324185 PRIMER_LEFT_1_SEQUENCE=GCATGTTTTCCTGCTTCCAT PRIMER_RIGHT_1_SEQUENCE=CTCACACTCACCCTCCCTGT PRIMER_INTERNAL_1_SEQUENCE=TGAGTTTCAGCGTGCTTCTG PRIMER_LEFT_1=188,20 PRIMER_RIGHT_1=275,20 PRIMER_INTERNAL_1=236,20 PRIMER_LEFT_1_TM=60.081 PRIMER_RIGHT_1_TM=60.153 PRIMER_INTERNAL_1_TM=60.324 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.1000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=88 PRIMER_PAIR_2_PENALTY=0.253280 PRIMER_LEFT_2_PENALTY=0.080962 PRIMER_RIGHT_2_PENALTY=0.172318 PRIMER_INTERNAL_2_PENALTY=0.362999 PRIMER_LEFT_2_SEQUENCE=GCATGTTTTCCTGCTTCCAT PRIMER_RIGHT_2_SEQUENCE=CTCACCCTCCCTGTCAGAAG PRIMER_INTERNAL_2_SEQUENCE=TGATGGAGAACAGGCACATC PRIMER_LEFT_2=188,20 PRIMER_RIGHT_2=269,20 PRIMER_INTERNAL_2=210,20 PRIMER_LEFT_2_TM=60.081 PRIMER_RIGHT_2_TM=59.828 PRIMER_INTERNAL_2_TM=59.637 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=82 PRIMER_PAIR_3_PENALTY=0.314291 PRIMER_LEFT_3_PENALTY=0.161348 PRIMER_RIGHT_3_PENALTY=0.152942 PRIMER_INTERNAL_3_PENALTY=0.324185 PRIMER_LEFT_3_SEQUENCE=TGCTTCCATGATGATGGAGA PRIMER_RIGHT_3_SEQUENCE=CTCACACTCACCCTCCCTGT PRIMER_INTERNAL_3_SEQUENCE=TGAGTTTCAGCGTGCTTCTG PRIMER_LEFT_3=199,20 PRIMER_RIGHT_3=275,20 PRIMER_INTERNAL_3=236,20 PRIMER_LEFT_3_TM=60.161 PRIMER_RIGHT_3_TM=60.153 PRIMER_INTERNAL_3_TM=60.324 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=10.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=8.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=77 PRIMER_PAIR_4_PENALTY=0.330418 PRIMER_LEFT_4_PENALTY=0.152942 PRIMER_RIGHT_4_PENALTY=0.177475 PRIMER_INTERNAL_4_PENALTY=4.128601 PRIMER_LEFT_4_SEQUENCE=ACAGGGAGGGTGAGTGTGAG PRIMER_RIGHT_4_SEQUENCE=GGAAATATGGCTCAGGCTGA PRIMER_INTERNAL_4_SEQUENCE=TGTAAGATGGTAGAGAGCCATCA PRIMER_LEFT_4=256,20 PRIMER_RIGHT_4=330,20 PRIMER_INTERNAL_4=288,23 PRIMER_LEFT_4_TM=60.153 PRIMER_RIGHT_4_TM=60.177 PRIMER_INTERNAL_4_TM=58.871 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=7.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=75 = SEQUENCE_ID=MH864 SEQUENCE_TEMPLATE=TGCCTGCAGGTCGACTCTAGAGGATCCCCTTCAGTGATAAGGACAGGTCTAGAACAAGCGTTCCCAACCCTGGCACCAATGACAGTTTGGACCAAATAACTCTTTGTTTCAGGGGACTGTCCTACACATTGTGGGATGTTTAGCAGCCTCCGTGGCTTCTACCCACTAGATGCCAGCAGCACAACACCCCTCCCCAACAATCATGACAATGAAAATGTCTTTAGACATTGCCAAATATACCTTGTGGGACAAAATGGCCCCTGATTGAGAACCACTGGTTTGGAGTATGGTTATGAGAAGTACAGTCTTGGCATGTGAAGTTCTGGAAATC SEQUENCE_INCLUDED_REGION=29,302 P3_COMMENT=CL is TTCAGTGATAAGGACAGGTCTA P3_COMMENT=CR is TTTCCAGAACTTCACATGCC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.400322 PRIMER_LEFT_0_PENALTY=0.151542 PRIMER_RIGHT_0_PENALTY=0.248781 PRIMER_INTERNAL_0_PENALTY=0.056936 PRIMER_LEFT_0_SEQUENCE=CTGGCACCAATGACAGTTTG PRIMER_RIGHT_0_SEQUENCE=CTAGTGGGTAGAAGCCACGG PRIMER_INTERNAL_0_SEQUENCE=TTTGTTTCAGGGGACTGTCC PRIMER_LEFT_0=69,20 PRIMER_RIGHT_0=168,20 PRIMER_INTERNAL_0=102,20 PRIMER_LEFT_0_TM=60.152 PRIMER_RIGHT_0_TM=59.751 PRIMER_INTERNAL_0_TM=59.943 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=7.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=9.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=0.435933 PRIMER_LEFT_1_PENALTY=0.056936 PRIMER_RIGHT_1_PENALTY=0.378997 PRIMER_INTERNAL_1_PENALTY=0.300243 PRIMER_LEFT_1_SEQUENCE=TTTGTTTCAGGGGACTGTCC PRIMER_RIGHT_1_SEQUENCE=TTGTGCTGCTGGCATCTAGT PRIMER_INTERNAL_1_SEQUENCE=GTGGGATGTTTAGCAGCCTC PRIMER_LEFT_1=102,20 PRIMER_RIGHT_1=183,20 PRIMER_INTERNAL_1=130,20 PRIMER_LEFT_1_TM=59.943 PRIMER_RIGHT_1_TM=59.621 PRIMER_INTERNAL_1_TM=59.700 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=7.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=5.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=0.435933 PRIMER_LEFT_2_PENALTY=0.056936 PRIMER_RIGHT_2_PENALTY=0.378997 PRIMER_INTERNAL_2_PENALTY=0.300243 PRIMER_LEFT_2_SEQUENCE=TTTGTTTCAGGGGACTGTCC PRIMER_RIGHT_2_SEQUENCE=GTTGTGCTGCTGGCATCTAG PRIMER_INTERNAL_2_SEQUENCE=GTGGGATGTTTAGCAGCCTC PRIMER_LEFT_2=102,20 PRIMER_RIGHT_2=184,20 PRIMER_INTERNAL_2=130,20 PRIMER_LEFT_2_TM=59.943 PRIMER_RIGHT_2_TM=59.621 PRIMER_INTERNAL_2_TM=59.700 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=7.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=5.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=83 PRIMER_PAIR_3_PENALTY=0.451785 PRIMER_LEFT_3_PENALTY=0.151542 PRIMER_RIGHT_3_PENALTY=0.300243 PRIMER_INTERNAL_3_PENALTY=0.056936 PRIMER_LEFT_3_SEQUENCE=CTGGCACCAATGACAGTTTG PRIMER_RIGHT_3_SEQUENCE=GAGGCTGCTAAACATCCCAC PRIMER_INTERNAL_3_SEQUENCE=TTTGTTTCAGGGGACTGTCC PRIMER_LEFT_3=69,20 PRIMER_RIGHT_3=149,20 PRIMER_INTERNAL_3=102,20 PRIMER_LEFT_3_TM=60.152 PRIMER_RIGHT_3_TM=59.700 PRIMER_INTERNAL_3_TM=59.943 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=7.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=81 PRIMER_PAIR_4_PENALTY=0.676879 PRIMER_LEFT_4_PENALTY=0.300243 PRIMER_RIGHT_4_PENALTY=0.376636 PRIMER_INTERNAL_4_PENALTY=0.378997 PRIMER_LEFT_4_SEQUENCE=GTGGGATGTTTAGCAGCCTC PRIMER_RIGHT_4_SEQUENCE=CATTGTCATGATTGTTGGGG PRIMER_INTERNAL_4_SEQUENCE=CTAGATGCCAGCAGCACAAC PRIMER_LEFT_4=130,20 PRIMER_RIGHT_4=210,20 PRIMER_INTERNAL_4=165,20 PRIMER_LEFT_4_TM=59.700 PRIMER_RIGHT_4_TM=59.623 PRIMER_INTERNAL_4_TM=59.621 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=11.2000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=81 = SEQUENCE_ID=MH866 SEQUENCE_TEMPLATE=TTCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCGATGGCTCCGACGGCTTGTATTCCAGCACAGCAGGGCTTGGCCGTTGTTGCAGACATTTGCCTTCTCTGCNTGGTGGCATGGACTCCATCCAGGACAGGTGTTTGGTTTCGTTTGCTGGGCCGTGATGGTGGAAG SEQUENCE_INCLUDED_REGION=33,137 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.023931 PRIMER_LEFT_0_PENALTY=0.018700 PRIMER_RIGHT_0_PENALTY=0.005232 PRIMER_INTERNAL_0_PENALTY=0.152999 PRIMER_LEFT_0_SEQUENCE=GCTTGTATTCCAGCACAGCA PRIMER_RIGHT_0_SEQUENCE=CGAAACCAAACACCTGTCCT PRIMER_INTERNAL_0_SEQUENCE=TGTTGCAGACATTTGCCTTC PRIMER_LEFT_0=48,20 PRIMER_RIGHT_0=145,20 PRIMER_INTERNAL_0=80,20 PRIMER_LEFT_0_TM=60.019 PRIMER_RIGHT_0_TM=60.005 PRIMER_INTERNAL_0_TM=59.847 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=98 PRIMER_PAIR_1_PENALTY=0.057144 PRIMER_LEFT_1_PENALTY=0.018700 PRIMER_RIGHT_1_PENALTY=0.038444 PRIMER_INTERNAL_1_PENALTY=0.152999 PRIMER_LEFT_1_SEQUENCE=GCTTGTATTCCAGCACAGCA PRIMER_RIGHT_1_SEQUENCE=AACACCTGTCCTGGATGGAG PRIMER_INTERNAL_1_SEQUENCE=TGTTGCAGACATTTGCCTTC PRIMER_LEFT_1=48,20 PRIMER_RIGHT_1=137,20 PRIMER_INTERNAL_1=80,20 PRIMER_LEFT_1_TM=60.019 PRIMER_RIGHT_1_TM=59.962 PRIMER_INTERNAL_1_TM=59.847 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=8.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=90 PRIMER_PAIR_2_PENALTY=0.141131 PRIMER_LEFT_2_PENALTY=0.135900 PRIMER_RIGHT_2_PENALTY=0.005232 PRIMER_INTERNAL_2_PENALTY=0.152999 PRIMER_LEFT_2_SEQUENCE=TTGTATTCCAGCACAGCAGG PRIMER_RIGHT_2_SEQUENCE=CGAAACCAAACACCTGTCCT PRIMER_INTERNAL_2_SEQUENCE=TGTTGCAGACATTTGCCTTC PRIMER_LEFT_2=50,20 PRIMER_RIGHT_2=145,20 PRIMER_INTERNAL_2=80,20 PRIMER_LEFT_2_TM=59.864 PRIMER_RIGHT_2_TM=60.005 PRIMER_INTERNAL_2_TM=59.847 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=96 PRIMER_PAIR_3_PENALTY=0.151269 PRIMER_LEFT_3_PENALTY=0.018700 PRIMER_RIGHT_3_PENALTY=0.132570 PRIMER_INTERNAL_3_PENALTY=0.152999 PRIMER_LEFT_3_SEQUENCE=GCTTGTATTCCAGCACAGCA PRIMER_RIGHT_3_SEQUENCE=ACGAAACCAAACACCTGTCC PRIMER_INTERNAL_3_SEQUENCE=TGTTGCAGACATTTGCCTTC PRIMER_LEFT_3=48,20 PRIMER_RIGHT_3=146,20 PRIMER_INTERNAL_3=80,20 PRIMER_LEFT_3_TM=60.019 PRIMER_RIGHT_3_TM=59.867 PRIMER_INTERNAL_3_TM=59.847 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=99 PRIMER_PAIR_4_PENALTY=0.157959 PRIMER_LEFT_4_PENALTY=0.018700 PRIMER_RIGHT_4_PENALTY=0.139259 PRIMER_INTERNAL_4_PENALTY=0.152999 PRIMER_LEFT_4_SEQUENCE=GCTTGTATTCCAGCACAGCA PRIMER_RIGHT_4_SEQUENCE=AAACCAAACACCTGTCCTGG PRIMER_INTERNAL_4_SEQUENCE=TGTTGCAGACATTTGCCTTC PRIMER_LEFT_4=48,20 PRIMER_RIGHT_4=143,20 PRIMER_INTERNAL_4=80,20 PRIMER_LEFT_4_TM=60.019 PRIMER_RIGHT_4_TM=59.861 PRIMER_INTERNAL_4_TM=59.847 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=5.00 PRIMER_PAIR_4_PRODUCT_SIZE=96 = SEQUENCE_ID=MH870 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGAAAGATGCAGGCGGAAGGTTTTTGTGCTGGAGACAATACAGGGGATGACTTCTTAAATCCGTCGTCGTCATCCTCCATTAGTGATAAATAGTCACCTTGGCTCAGATTCGTCAGACAAATACTATATTGCTACTTGTTATCTTCTAGTAACCTTCAGAAAAACTTTAGTGTTTACTTAAAAAATGAGGAAAAAAAAGAATCATTATGTAGAAAAAAATGAGAACATTTTGCTTCGTTTCAAAAATTATTTATTCAAATTAGTGAACAAAACATGGTCTATCGGAATAGGCTCATTAAACAG SEQUENCE_INCLUDED_REGION=34,303 P3_COMMENT=CL is GAAAGATGCAGGCGGAAG P3_COMMENT=CR is TGTTTAATGAGCCTATTCCG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.629967 PRIMER_LEFT_0_PENALTY=0.072951 PRIMER_RIGHT_0_PENALTY=0.557015 PRIMER_INTERNAL_0_PENALTY=0.727969 PRIMER_LEFT_0_SEQUENCE=GCTGGAGACAATACAGGGGA PRIMER_RIGHT_0_SEQUENCE=TGTCTGACGAATCTGAGCCA PRIMER_INTERNAL_0_SEQUENCE=TCTTAAATCCGTCGTCGTCA PRIMER_LEFT_0=61,20 PRIMER_RIGHT_0=152,20 PRIMER_INTERNAL_0=86,20 PRIMER_LEFT_0_TM=60.073 PRIMER_RIGHT_0_TM=60.557 PRIMER_INTERNAL_0_TM=59.272 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=92 PRIMER_PAIR_1_PENALTY=0.874143 PRIMER_LEFT_1_PENALTY=0.072951 PRIMER_RIGHT_1_PENALTY=0.801192 PRIMER_INTERNAL_1_PENALTY=0.727969 PRIMER_LEFT_1_SEQUENCE=GCTGGAGACAATACAGGGGA PRIMER_RIGHT_1_SEQUENCE=CGAATCTGAGCCAAGGTGAC PRIMER_INTERNAL_1_SEQUENCE=TCTTAAATCCGTCGTCGTCA PRIMER_LEFT_1=61,20 PRIMER_RIGHT_1=145,20 PRIMER_INTERNAL_1=86,20 PRIMER_LEFT_1_TM=60.073 PRIMER_RIGHT_1_TM=60.801 PRIMER_INTERNAL_1_TM=59.272 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=10.9000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=85 PRIMER_PAIR_2_PENALTY=0.874143 PRIMER_LEFT_2_PENALTY=0.072951 PRIMER_RIGHT_2_PENALTY=0.801192 PRIMER_INTERNAL_2_PENALTY=0.727969 PRIMER_LEFT_2_SEQUENCE=GCTGGAGACAATACAGGGGA PRIMER_RIGHT_2_SEQUENCE=ACGAATCTGAGCCAAGGTGA PRIMER_INTERNAL_2_SEQUENCE=TCTTAAATCCGTCGTCGTCA PRIMER_LEFT_2=61,20 PRIMER_RIGHT_2=146,20 PRIMER_INTERNAL_2=86,20 PRIMER_LEFT_2_TM=60.073 PRIMER_RIGHT_2_TM=60.801 PRIMER_INTERNAL_2_TM=59.272 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=10.9000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=86 PRIMER_PAIR_3_PENALTY=0.874143 PRIMER_LEFT_3_PENALTY=0.072951 PRIMER_RIGHT_3_PENALTY=0.801192 PRIMER_INTERNAL_3_PENALTY=0.727969 PRIMER_LEFT_3_SEQUENCE=GCTGGAGACAATACAGGGGA PRIMER_RIGHT_3_SEQUENCE=GACGAATCTGAGCCAAGGTG PRIMER_INTERNAL_3_SEQUENCE=TCTTAAATCCGTCGTCGTCA PRIMER_LEFT_3=61,20 PRIMER_RIGHT_3=147,20 PRIMER_INTERNAL_3=86,20 PRIMER_LEFT_3_TM=60.073 PRIMER_RIGHT_3_TM=60.801 PRIMER_INTERNAL_3_TM=59.272 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=87 PRIMER_PAIR_4_PENALTY=0.874143 PRIMER_LEFT_4_PENALTY=0.072951 PRIMER_RIGHT_4_PENALTY=0.801192 PRIMER_INTERNAL_4_PENALTY=0.727969 PRIMER_LEFT_4_SEQUENCE=GCTGGAGACAATACAGGGGA PRIMER_RIGHT_4_SEQUENCE=TGACGAATCTGAGCCAAGGT PRIMER_INTERNAL_4_SEQUENCE=TCTTAAATCCGTCGTCGTCA PRIMER_LEFT_4=61,20 PRIMER_RIGHT_4=148,20 PRIMER_INTERNAL_4=86,20 PRIMER_LEFT_4_TM=60.073 PRIMER_RIGHT_4_TM=60.801 PRIMER_INTERNAL_4_TM=59.272 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=10.9000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=88 = SEQUENCE_ID=MH877 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCTTGAGATCAGTCCTCATCTTATTTTATATCCTCAGCTTCTAACATGTTGTCTGGTGCATAGTTGGCACTAAATAAATATTTTTGAATGAATTAAGTAATGTCAGCAGATGACTAGATATTATATGCTTCTTGAATTCTTTCCTAATCTCTTCAACCACCACACCTATCTCCCACCCTACTGAGAGTTGAGTATTTCCTCTTCTGTATGTCAATGAAAATTTGCAAGTAACTTAGGTGACCAATCATATACCCAGGTTTACCTGAGACATCCCTAGTTTATGCCTATTGTTCTTGCATAACTATT SEQUENCE_INCLUDED_REGION=33,308 P3_COMMENT=CL is CTCCTTGAGATCAGTCCTCA P3_COMMENT=CR is AGTTATGCAAGAACAATAGGCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.557213 PRIMER_LEFT_0_PENALTY=0.237616 PRIMER_RIGHT_0_PENALTY=2.319597 PRIMER_INTERNAL_0_PENALTY=4.462902 PRIMER_LEFT_0_SEQUENCE=CACCACACCTATCTCCCACC PRIMER_RIGHT_0_SEQUENCE=CCTGGGTATATGATTGGTCACC PRIMER_INTERNAL_0_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA PRIMER_LEFT_0=192,20 PRIMER_RIGHT_0=291,22 PRIMER_INTERNAL_0=237,24 PRIMER_LEFT_0_TM=60.238 PRIMER_RIGHT_0_TM=60.320 PRIMER_INTERNAL_0_TM=59.537 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=29.167 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=3.351604 PRIMER_LEFT_1_PENALTY=1.032007 PRIMER_RIGHT_1_PENALTY=2.319597 PRIMER_INTERNAL_1_PENALTY=4.462902 PRIMER_LEFT_1_SEQUENCE=ACCACACCTATCTCCCACCC PRIMER_RIGHT_1_SEQUENCE=CCTGGGTATATGATTGGTCACC PRIMER_INTERNAL_1_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA PRIMER_LEFT_1=193,20 PRIMER_RIGHT_1=291,22 PRIMER_INTERNAL_1=237,24 PRIMER_LEFT_1_TM=61.032 PRIMER_RIGHT_1_TM=60.320 PRIMER_INTERNAL_1_TM=59.537 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=29.167 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=8.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=3.479771 PRIMER_LEFT_2_PENALTY=1.160174 PRIMER_RIGHT_2_PENALTY=2.319597 PRIMER_INTERNAL_2_PENALTY=4.462902 PRIMER_LEFT_2_SEQUENCE=CCACACCTATCTCCCACCCT PRIMER_RIGHT_2_SEQUENCE=CCTGGGTATATGATTGGTCACC PRIMER_INTERNAL_2_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA PRIMER_LEFT_2=194,20 PRIMER_RIGHT_2=291,22 PRIMER_INTERNAL_2=237,24 PRIMER_LEFT_2_TM=61.160 PRIMER_RIGHT_2_TM=60.320 PRIMER_INTERNAL_2_TM=59.537 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=29.167 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=8.00 PRIMER_LEFT_2_END_STABILITY=9.1000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=98 PRIMER_PAIR_3_PENALTY=3.502965 PRIMER_LEFT_3_PENALTY=1.183368 PRIMER_RIGHT_3_PENALTY=2.319597 PRIMER_INTERNAL_3_PENALTY=4.462902 PRIMER_LEFT_3_SEQUENCE=CCACACCTATCTCCCACCC PRIMER_RIGHT_3_SEQUENCE=CCTGGGTATATGATTGGTCACC PRIMER_INTERNAL_3_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA PRIMER_LEFT_3=194,19 PRIMER_RIGHT_3=291,22 PRIMER_INTERNAL_3=237,24 PRIMER_LEFT_3_TM=60.183 PRIMER_RIGHT_3_TM=60.320 PRIMER_INTERNAL_3_TM=59.537 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=29.167 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=8.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=3.613467 PRIMER_LEFT_4_PENALTY=1.293870 PRIMER_RIGHT_4_PENALTY=2.319597 PRIMER_INTERNAL_4_PENALTY=4.462902 PRIMER_LEFT_4_SEQUENCE=TCCCACCCTACTGAGAGTTGA PRIMER_RIGHT_4_SEQUENCE=CCTGGGTATATGATTGGTCACC PRIMER_INTERNAL_4_SEQUENCE=TCTGTATGTCAATGAAAATTTGCA PRIMER_LEFT_4=205,21 PRIMER_RIGHT_4=291,22 PRIMER_INTERNAL_4=237,24 PRIMER_LEFT_4_TM=59.706 PRIMER_RIGHT_4_TM=60.320 PRIMER_INTERNAL_4_TM=59.537 PRIMER_LEFT_4_GC_PERCENT=52.381 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=29.167 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=8.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH880 SEQUENCE_TEMPLATE=TGCCTGCAGGTCGACTCTAGAGGATCCCCTTTTCGTTTTGGGGTGGAAAACTTGTATCTGAAATGTTCCTGTGAATGTTAAGAAAGTTAAAAATGAATTATTTTTCTCTTCTTTAATGCCCATCCATTTTTTGTCTTCATCTGAGAATGACTTTCCTAAAGACAACATTCGTATACAGAAGCCAGACCTATGCAAGAGAAAGTATAATAATTTTCAATGAGGCAAAGGTCATTAATATGACCTACATCTTCATAAAATGCGAATTTAATCACGAGTCATGACGATTGTTCTTTGAGAGGCTTTTTATTCCCCATGGTGAAAAAAGATTTGTAGT SEQUENCE_INCLUDED_REGION=29,305 P3_COMMENT=CL is TCGTTTTGGGGTGGAAAACT P3_COMMENT=CR is TACAAATCTTTTTTCACCATGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.064566 PRIMER_LEFT_0_PENALTY=0.692222 PRIMER_RIGHT_0_PENALTY=0.372343 PRIMER_INTERNAL_0_PENALTY=4.384367 PRIMER_LEFT_0_SEQUENCE=TTTTGGGGTGGAAAACTTGT PRIMER_RIGHT_0_SEQUENCE=GACAAAAAATGGATGGGCAT PRIMER_INTERNAL_0_SEQUENCE=TCTGAAATGTTCCTGTGAATGTT PRIMER_LEFT_0=35,20 PRIMER_RIGHT_0=134,20 PRIMER_INTERNAL_0=56,23 PRIMER_LEFT_0_TM=59.308 PRIMER_RIGHT_0_TM=59.628 PRIMER_INTERNAL_0_TM=58.616 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=9.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=1.284986 PRIMER_LEFT_1_PENALTY=0.309369 PRIMER_RIGHT_1_PENALTY=0.975617 PRIMER_INTERNAL_1_PENALTY=4.297164 PRIMER_LEFT_1_SEQUENCE=TGCCCATCCATTTTTTGTCT PRIMER_RIGHT_1_SEQUENCE=TTGCATAGGTCTGGCTTCTG PRIMER_INTERNAL_1_SEQUENCE=TGACTTTCCTAAAGACAACATTCG PRIMER_LEFT_1=116,20 PRIMER_RIGHT_1=194,20 PRIMER_INTERNAL_1=147,24 PRIMER_LEFT_1_TM=60.309 PRIMER_RIGHT_1_TM=59.024 PRIMER_INTERNAL_1_TM=59.703 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=79 PRIMER_PAIR_2_PENALTY=1.332157 PRIMER_LEFT_2_PENALTY=0.309369 PRIMER_RIGHT_2_PENALTY=1.022788 PRIMER_INTERNAL_2_PENALTY=4.297164 PRIMER_LEFT_2_SEQUENCE=TGCCCATCCATTTTTTGTCT PRIMER_RIGHT_2_SEQUENCE=CTTGCATAGGTCTGGCTTCTG PRIMER_INTERNAL_2_SEQUENCE=TGACTTTCCTAAAGACAACATTCG PRIMER_LEFT_2=116,20 PRIMER_RIGHT_2=195,21 PRIMER_INTERNAL_2=147,24 PRIMER_LEFT_2_TM=60.309 PRIMER_RIGHT_2_TM=60.023 PRIMER_INTERNAL_2_TM=59.703 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_INTERNAL_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=1.347960 PRIMER_LEFT_3_PENALTY=0.372343 PRIMER_RIGHT_3_PENALTY=0.975617 PRIMER_INTERNAL_3_PENALTY=4.297164 PRIMER_LEFT_3_SEQUENCE=ATGCCCATCCATTTTTTGTC PRIMER_RIGHT_3_SEQUENCE=TTGCATAGGTCTGGCTTCTG PRIMER_INTERNAL_3_SEQUENCE=TGACTTTCCTAAAGACAACATTCG PRIMER_LEFT_3=115,20 PRIMER_RIGHT_3=194,20 PRIMER_INTERNAL_3=147,24 PRIMER_LEFT_3_TM=59.628 PRIMER_RIGHT_3_TM=59.024 PRIMER_INTERNAL_3_TM=59.703 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=37.500 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=1.395132 PRIMER_LEFT_4_PENALTY=0.372343 PRIMER_RIGHT_4_PENALTY=1.022788 PRIMER_INTERNAL_4_PENALTY=4.297164 PRIMER_LEFT_4_SEQUENCE=ATGCCCATCCATTTTTTGTC PRIMER_RIGHT_4_SEQUENCE=CTTGCATAGGTCTGGCTTCTG PRIMER_INTERNAL_4_SEQUENCE=TGACTTTCCTAAAGACAACATTCG PRIMER_LEFT_4=115,20 PRIMER_RIGHT_4=195,21 PRIMER_INTERNAL_4=147,24 PRIMER_LEFT_4_TM=59.628 PRIMER_RIGHT_4_TM=60.023 PRIMER_INTERNAL_4_TM=59.703 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_INTERNAL_4_GC_PERCENT=37.500 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=81 = SEQUENCE_ID=MH881 SEQUENCE_TEMPLATE=ATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCAGTTTCAACTGGAGAGATCTAGATAGCATTAAGTGTTTATTTAGGTGTGGCAGACTGAATCCTCTTGACCTGTGAACATATACTATTTGACTAGAGTNATGTTTTGCCTTTTTAAATTAAATTTGAGTAGTTTTGGTTGGAGCATTACTACTATTTGCAACAATCCTCAAAACTCCCTATTTATTGCTTTTTGCTTGGCTCACTTCACATCCATTGTAGGCATTTGTCTGTGTTCTGGTTTAATGCTGTGAGTTTTAATAAGAGTTTCTGTACGGAGCCTGAGATATCTCTATAAGAAGAAAAA SEQUENCE_INCLUDED_REGION=30,272 P3_COMMENT=CL is TTTCAACTGGAGAGATCTAGATAGC P3_COMMENT=CR is GAAGTGAGCCAAGCAAAAAG P3_COMMENT=CM is TTAAGTGTTTATTTAGGTGTGGCA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.964268 PRIMER_LEFT_0_PENALTY=1.103105 PRIMER_RIGHT_0_PENALTY=0.861164 PRIMER_INTERNAL_0_PENALTY=0.008461 PRIMER_LEFT_0_SEQUENCE=TGCAACAATCCTCAAAACTCC PRIMER_RIGHT_0_SEQUENCE=CCAGAACACAGACAAATGCC PRIMER_INTERNAL_0_SEQUENCE=TGCTTGGCTCACTTCACATC PRIMER_LEFT_0=188,21 PRIMER_RIGHT_0=270,20 PRIMER_INTERNAL_0=224,20 PRIMER_LEFT_0_TM=60.103 PRIMER_RIGHT_0_TM=59.139 PRIMER_INTERNAL_0_TM=59.992 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=9.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=83 PRIMER_PAIR_1_PENALTY=2.130697 PRIMER_LEFT_1_PENALTY=1.103105 PRIMER_RIGHT_1_PENALTY=1.027592 PRIMER_INTERNAL_1_PENALTY=0.008461 PRIMER_LEFT_1_SEQUENCE=TGCAACAATCCTCAAAACTCC PRIMER_RIGHT_1_SEQUENCE=ACCAGAACACAGACAAATGCC PRIMER_INTERNAL_1_SEQUENCE=TGCTTGGCTCACTTCACATC PRIMER_LEFT_1=188,21 PRIMER_RIGHT_1=271,21 PRIMER_INTERNAL_1=224,20 PRIMER_LEFT_1_TM=60.103 PRIMER_RIGHT_1_TM=60.028 PRIMER_INTERNAL_1_TM=59.992 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=9.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=84 PRIMER_PAIR_2_PENALTY=2.223677 PRIMER_LEFT_2_PENALTY=2.215215 PRIMER_RIGHT_2_PENALTY=0.008461 PRIMER_INTERNAL_2_PENALTY=1.103105 PRIMER_LEFT_2_SEQUENCE=TTGAGTAGTTTTGGTTGGAGCA PRIMER_RIGHT_2_SEQUENCE=GATGTGAAGTGAGCCAAGCA PRIMER_INTERNAL_2_SEQUENCE=TGCAACAATCCTCAAAACTCC PRIMER_LEFT_2=155,22 PRIMER_RIGHT_2=243,20 PRIMER_INTERNAL_2=188,21 PRIMER_LEFT_2_TM=59.785 PRIMER_RIGHT_2_TM=59.992 PRIMER_INTERNAL_2_TM=60.103 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=5.00 PRIMER_PAIR_2_PRODUCT_SIZE=89 PRIMER_PAIR_3_PENALTY=2.266708 PRIMER_LEFT_3_PENALTY=1.103105 PRIMER_RIGHT_3_PENALTY=1.163603 PRIMER_INTERNAL_3_PENALTY=0.008461 PRIMER_LEFT_3_SEQUENCE=TGCAACAATCCTCAAAACTCC PRIMER_RIGHT_3_SEQUENCE=CCAGAACACAGACAAATGCCT PRIMER_INTERNAL_3_SEQUENCE=TGCTTGGCTCACTTCACATC PRIMER_LEFT_3=188,21 PRIMER_RIGHT_3=270,21 PRIMER_INTERNAL_3=224,20 PRIMER_LEFT_3_TM=60.103 PRIMER_RIGHT_3_TM=60.164 PRIMER_INTERNAL_3_TM=59.992 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=2.384687 PRIMER_LEFT_4_PENALTY=2.215215 PRIMER_RIGHT_4_PENALTY=0.169471 PRIMER_INTERNAL_4_PENALTY=1.103105 PRIMER_LEFT_4_SEQUENCE=TTGAGTAGTTTTGGTTGGAGCA PRIMER_RIGHT_4_SEQUENCE=TGGATGTGAAGTGAGCCAAG PRIMER_INTERNAL_4_SEQUENCE=TGCAACAATCCTCAAAACTCC PRIMER_LEFT_4=155,22 PRIMER_RIGHT_4=245,20 PRIMER_INTERNAL_4=188,21 PRIMER_LEFT_4_TM=59.785 PRIMER_RIGHT_4_TM=59.831 PRIMER_INTERNAL_4_TM=60.103 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH889 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACCTCCTGTTTGCTCATTTTGAAGAGACTCATTACGGACAATGGTCCTCAATGTTAATGAAGTGACACAATCCAATTAGAAGATTGCAGTTACACATGGTAGTGGTAGAATGAGAGGANTTGCTACCTAACAGGTAATGTCACTGTTTGTGCCTTGTTTTTGCCCTCTGTGGGGTTGTTTCTGCTTTGGTCCAGATCTCACTCTGTCCTTGGGCCTACTTTGGGATTCTGCCATCTTCTGAATGCTGGTAAATGCCTAAG SEQUENCE_INCLUDED_REGION=36,262 P3_COMMENT=CL is CTCATTACGGACAATGGTCC P3_COMMENT=CR is AGGCATTTACCAGCATTCAG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.261146 PRIMER_LEFT_0_PENALTY=0.247539 PRIMER_RIGHT_0_PENALTY=0.013607 PRIMER_INTERNAL_0_PENALTY=0.148509 PRIMER_LEFT_0_SEQUENCE=TGTTTGTGCCTTGTTTTTGC PRIMER_RIGHT_0_SEQUENCE=GAAGATGGCAGAATCCCAAA PRIMER_INTERNAL_0_SEQUENCE=CTGTGGGGTTGTTTCTGCTT PRIMER_LEFT_0=181,20 PRIMER_RIGHT_0=275,20 PRIMER_INTERNAL_0=204,20 PRIMER_LEFT_0_TM=59.752 PRIMER_RIGHT_0_TM=60.014 PRIMER_INTERNAL_0_TM=60.149 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=95 PRIMER_PAIR_1_PENALTY=0.306236 PRIMER_LEFT_1_PENALTY=0.148509 PRIMER_RIGHT_1_PENALTY=0.157727 PRIMER_INTERNAL_1_PENALTY=0.278847 PRIMER_LEFT_1_SEQUENCE=CTGTGGGGTTGTTTCTGCTT PRIMER_RIGHT_1_SEQUENCE=TCAGAAGATGGCAGAATCCC PRIMER_INTERNAL_1_SEQUENCE=CACTCTGTCCTTGGGCCTAC PRIMER_LEFT_1=204,20 PRIMER_RIGHT_1=278,20 PRIMER_INTERNAL_1=236,20 PRIMER_LEFT_1_TM=60.149 PRIMER_RIGHT_1_TM=60.158 PRIMER_INTERNAL_1_TM=59.721 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=9.3000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=0.313862 PRIMER_LEFT_2_PENALTY=0.247539 PRIMER_RIGHT_2_PENALTY=0.066323 PRIMER_INTERNAL_2_PENALTY=0.148509 PRIMER_LEFT_2_SEQUENCE=TGTTTGTGCCTTGTTTTTGC PRIMER_RIGHT_2_SEQUENCE=TGGCAGAATCCCAAAGTAGG PRIMER_INTERNAL_2_SEQUENCE=CTGTGGGGTTGTTTCTGCTT PRIMER_LEFT_2=181,20 PRIMER_RIGHT_2=270,20 PRIMER_INTERNAL_2=204,20 PRIMER_LEFT_2_TM=59.752 PRIMER_RIGHT_2_TM=60.066 PRIMER_INTERNAL_2_TM=60.149 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.8000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=90 PRIMER_PAIR_3_PENALTY=0.374652 PRIMER_LEFT_3_PENALTY=0.148509 PRIMER_RIGHT_3_PENALTY=0.226143 PRIMER_INTERNAL_3_PENALTY=0.271680 PRIMER_LEFT_3_SEQUENCE=CTGTGGGGTTGTTTCTGCTT PRIMER_RIGHT_3_SEQUENCE=ACCAGCATTCAGAAGATGGC PRIMER_INTERNAL_3_SEQUENCE=CTGTCCTTGGGCCTACTTTG PRIMER_LEFT_3=204,20 PRIMER_RIGHT_3=286,20 PRIMER_INTERNAL_3=240,20 PRIMER_LEFT_3_TM=60.149 PRIMER_RIGHT_3_TM=60.226 PRIMER_INTERNAL_3_TM=59.728 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=9.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=83 PRIMER_PAIR_4_PENALTY=0.405266 PRIMER_LEFT_4_PENALTY=0.247539 PRIMER_RIGHT_4_PENALTY=0.157727 PRIMER_INTERNAL_4_PENALTY=0.148509 PRIMER_LEFT_4_SEQUENCE=TGTTTGTGCCTTGTTTTTGC PRIMER_RIGHT_4_SEQUENCE=TCAGAAGATGGCAGAATCCC PRIMER_INTERNAL_4_SEQUENCE=CTGTGGGGTTGTTTCTGCTT PRIMER_LEFT_4=181,20 PRIMER_RIGHT_4=278,20 PRIMER_INTERNAL_4=204,20 PRIMER_LEFT_4_TM=59.752 PRIMER_RIGHT_4_TM=60.158 PRIMER_INTERNAL_4_TM=60.149 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.8000 PRIMER_RIGHT_4_END_STABILITY=9.3000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH901 SEQUENCE_TEMPLATE=AGGGAAACTTCTCTGTGAGANNAGGATCAGGGCATGGCTGGGTGGGAAAGCCAGGAGGGTTTCCTGAAGGAGACATCACCCTAGTCTAATAATTAGCTTTAATTATCCTCACCACAGCCCTAGAAGGAAGGCACTGNTTATCTCTCATTTTACTTTTCTCTGAAGTTAAGAGAAATTAAGTGACTTGCCCAAGGTGATGGAG SEQUENCE_INCLUDED_REGION=0,202 P3_COMMENT=CL is GGGTTTCCTGAAGGAGACAT P3_COMMENT=CR is ACCTTGGGCAAGTCACTTAA P3_COMMENT=CM is ATTAGCTTTAATTATCCTCACCACA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.468578 PRIMER_LEFT_0_PENALTY=0.329319 PRIMER_RIGHT_0_PENALTY=0.139259 PRIMER_INTERNAL_0_PENALTY=9.921269 PRIMER_LEFT_0_SEQUENCE=AGGAGGGTTTCCTGAAGGAG PRIMER_RIGHT_0_SEQUENCE=CCTTCTAGGGCTGTGGTGAG PRIMER_INTERNAL_0_SEQUENCE=CATCACCCTAGTCTAATAATTAGCTTT PRIMER_LEFT_0=52,20 PRIMER_RIGHT_0=126,20 PRIMER_INTERNAL_0=73,27 PRIMER_LEFT_0_TM=59.671 PRIMER_RIGHT_0_TM=59.861 PRIMER_INTERNAL_0_TM=57.079 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=33.333 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=9.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=0.560031 PRIMER_LEFT_1_PENALTY=0.139259 PRIMER_RIGHT_1_PENALTY=0.420772 PRIMER_INTERNAL_1_PENALTY=9.339364 PRIMER_LEFT_1_SEQUENCE=CTCACCACAGCCCTAGAAGG PRIMER_RIGHT_1_SEQUENCE=ATCACCTTGGGCAAGTCACT PRIMER_INTERNAL_1_SEQUENCE=TCTCTCATTTTACTTTTCTCTGAAGTT PRIMER_LEFT_1=107,20 PRIMER_RIGHT_1=197,20 PRIMER_INTERNAL_1=140,27 PRIMER_LEFT_1_TM=59.861 PRIMER_RIGHT_1_TM=59.579 PRIMER_INTERNAL_1_TM=57.661 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=29.630 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=91 PRIMER_PAIR_2_PENALTY=0.560031 PRIMER_LEFT_2_PENALTY=0.139259 PRIMER_RIGHT_2_PENALTY=0.420772 PRIMER_INTERNAL_2_PENALTY=9.339364 PRIMER_LEFT_2_SEQUENCE=CCTCACCACAGCCCTAGAAG PRIMER_RIGHT_2_SEQUENCE=ATCACCTTGGGCAAGTCACT PRIMER_INTERNAL_2_SEQUENCE=TCTCTCATTTTACTTTTCTCTGAAGTT PRIMER_LEFT_2=106,20 PRIMER_RIGHT_2=197,20 PRIMER_INTERNAL_2=140,27 PRIMER_LEFT_2_TM=59.861 PRIMER_RIGHT_2_TM=59.579 PRIMER_INTERNAL_2_TM=57.661 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=29.630 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=92 PRIMER_PAIR_3_PENALTY=0.575757 PRIMER_LEFT_3_PENALTY=0.329319 PRIMER_RIGHT_3_PENALTY=0.246438 PRIMER_INTERNAL_3_PENALTY=9.921269 PRIMER_LEFT_3_SEQUENCE=AGGAGGGTTTCCTGAAGGAG PRIMER_RIGHT_3_SEQUENCE=TTCCTTCTAGGGCTGTGGTG PRIMER_INTERNAL_3_SEQUENCE=CATCACCCTAGTCTAATAATTAGCTTT PRIMER_LEFT_3=52,20 PRIMER_RIGHT_3=128,20 PRIMER_INTERNAL_3=73,27 PRIMER_LEFT_3_TM=59.671 PRIMER_RIGHT_3_TM=60.246 PRIMER_INTERNAL_3_TM=57.079 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=33.333 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=9.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=77 PRIMER_PAIR_4_PENALTY=0.667209 PRIMER_LEFT_4_PENALTY=0.246438 PRIMER_RIGHT_4_PENALTY=0.420772 PRIMER_INTERNAL_4_PENALTY=9.339364 PRIMER_LEFT_4_SEQUENCE=CACCACAGCCCTAGAAGGAA PRIMER_RIGHT_4_SEQUENCE=ATCACCTTGGGCAAGTCACT PRIMER_INTERNAL_4_SEQUENCE=TCTCTCATTTTACTTTTCTCTGAAGTT PRIMER_LEFT_4=109,20 PRIMER_RIGHT_4=197,20 PRIMER_INTERNAL_4=140,27 PRIMER_LEFT_4_TM=60.246 PRIMER_RIGHT_4_TM=59.579 PRIMER_INTERNAL_4_TM=57.661 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=29.630 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH903 SEQUENCE_TEMPLATE=CATGGTGTACTACCTGGGTNTTGATAATGGTTATTCAGGGACCAAGGGCACTTTAGTCAGAAGGTGATTGGTCCTGCCAGANCTGAGTCCATCGCTTCATGGCAGTGGGTTCCCTTGTGGCCCAGGGTGTGTCTAGAAATGTTGTCCAGGAG SEQUENCE_INCLUDED_REGION=0,152 P3_COMMENT=CL is TTGATAATGGTTATTCAGGGA P3_COMMENT=CR is CCTGGACAACATTTCTAGACA P3_COMMENT=CM is CAAGGGCACTTTAGTCAGAAGG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.767470 PRIMER_LEFT_0_PENALTY=0.656393 PRIMER_RIGHT_0_PENALTY=0.111077 PRIMER_INTERNAL_0_PENALTY=1.772556 PRIMER_LEFT_0_SEQUENCE=CAGAAGGTGATTGGTCCTGC PRIMER_RIGHT_0_SEQUENCE=TTTCTAGACACACCCTGGGC PRIMER_INTERNAL_0_SEQUENCE=CTGAGTCCATCGCTTCATGG PRIMER_LEFT_0=57,20 PRIMER_RIGHT_0=138,20 PRIMER_INTERNAL_0=82,20 PRIMER_LEFT_0_TM=60.656 PRIMER_RIGHT_0_TM=60.111 PRIMER_INTERNAL_0_TM=61.773 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=11.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=1.470028 PRIMER_LEFT_1_PENALTY=1.358951 PRIMER_RIGHT_1_PENALTY=0.111077 PRIMER_INTERNAL_1_PENALTY=1.772556 PRIMER_LEFT_1_SEQUENCE=TCAGAAGGTGATTGGTCCTG PRIMER_RIGHT_1_SEQUENCE=TTTCTAGACACACCCTGGGC PRIMER_INTERNAL_1_SEQUENCE=CTGAGTCCATCGCTTCATGG PRIMER_LEFT_1=56,20 PRIMER_RIGHT_1=138,20 PRIMER_INTERNAL_1=82,20 PRIMER_LEFT_1_TM=58.641 PRIMER_RIGHT_1_TM=60.111 PRIMER_INTERNAL_1_TM=61.773 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=11.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=1.540483 PRIMER_LEFT_2_PENALTY=1.429406 PRIMER_RIGHT_2_PENALTY=0.111077 PRIMER_INTERNAL_2_PENALTY=1.772556 PRIMER_LEFT_2_SEQUENCE=AGAAGGTGATTGGTCCTGCC PRIMER_RIGHT_2_SEQUENCE=TTTCTAGACACACCCTGGGC PRIMER_INTERNAL_2_SEQUENCE=CTGAGTCCATCGCTTCATGG PRIMER_LEFT_2=58,20 PRIMER_RIGHT_2=138,20 PRIMER_INTERNAL_2=82,20 PRIMER_LEFT_2_TM=61.429 PRIMER_RIGHT_2_TM=60.111 PRIMER_INTERNAL_2_TM=61.773 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=11.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=1.546895 PRIMER_LEFT_3_PENALTY=1.435818 PRIMER_RIGHT_3_PENALTY=0.111077 PRIMER_INTERNAL_3_PENALTY=1.772556 PRIMER_LEFT_3_SEQUENCE=GTCAGAAGGTGATTGGTCCTG PRIMER_RIGHT_3_SEQUENCE=TTTCTAGACACACCCTGGGC PRIMER_INTERNAL_3_SEQUENCE=CTGAGTCCATCGCTTCATGG PRIMER_LEFT_3=55,21 PRIMER_RIGHT_3=138,20 PRIMER_INTERNAL_3=82,20 PRIMER_LEFT_3_TM=59.564 PRIMER_RIGHT_3_TM=60.111 PRIMER_INTERNAL_3_TM=61.773 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=11.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=84 PRIMER_PAIR_4_PENALTY=1.579343 PRIMER_LEFT_4_PENALTY=1.468266 PRIMER_RIGHT_4_PENALTY=0.111077 PRIMER_INTERNAL_4_PENALTY=1.772556 PRIMER_LEFT_4_SEQUENCE=GAAGGTGATTGGTCCTGCC PRIMER_RIGHT_4_SEQUENCE=TTTCTAGACACACCCTGGGC PRIMER_INTERNAL_4_SEQUENCE=CTGAGTCCATCGCTTCATGG PRIMER_LEFT_4=59,19 PRIMER_RIGHT_4=138,20 PRIMER_INTERNAL_4=82,20 PRIMER_LEFT_4_TM=60.468 PRIMER_RIGHT_4_TM=60.111 PRIMER_INTERNAL_4_TM=61.773 PRIMER_LEFT_4_GC_PERCENT=57.895 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=11.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=80 = SEQUENCE_ID=MH904 SEQUENCE_TEMPLATE=GCTTCATGCTGCAGGTCGACTCTAGAGGATCCCCCTGATTTTACTGCTTTTNCTTTCTGTTTAACTTTCCTTTCCTTTTCTCCCTTGGTTAGAGTGTGGGACACTAGATCAGCAAAATTAACAAGATCATGAGTTTGAGAGGTAGCCCAAAGGGGGCATTCATTGTCTCTTTATTATAATTGCCAATTCTTCTTCCAATCTGTTTACAAAGTTGAATAAAGGGGATTCAAGTTGATTTTGGTATGATTTTGGTGGTTAGTATAACCTTCTTCTGATAGGCCTAAATGTTATTTAAAGACTTTTGTAAAGATCTTTTATAGTATGACACTACAGGCTC SEQUENCE_INCLUDED_REGION=34,303 P3_COMMENT=CL is TTTCCTTTTCTCCCTTGGTT P3_COMMENT=CR is TCAGAAGAAGGTTATACTAACCACC P3_COMMENT=CM is GAGTGTGGGACACTAGATCAGC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.731753 PRIMER_LEFT_0_PENALTY=0.039531 PRIMER_RIGHT_0_PENALTY=0.692222 PRIMER_INTERNAL_0_PENALTY=1.696755 PRIMER_LEFT_0_SEQUENCE=CCTTTCCTTTTCTCCCTTGG PRIMER_RIGHT_0_SEQUENCE=CCTTTGGGCTACCTCTCAAA PRIMER_INTERNAL_0_SEQUENCE=TGTGGGACACTAGATCAGCAA PRIMER_LEFT_0=68,20 PRIMER_RIGHT_0=152,20 PRIMER_INTERNAL_0=94,21 PRIMER_LEFT_0_TM=60.040 PRIMER_RIGHT_0_TM=59.308 PRIMER_INTERNAL_0_TM=59.303 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=85 PRIMER_PAIR_1_PENALTY=0.732229 PRIMER_LEFT_1_PENALTY=0.039531 PRIMER_RIGHT_1_PENALTY=0.692698 PRIMER_INTERNAL_1_PENALTY=1.696755 PRIMER_LEFT_1_SEQUENCE=CCTTTCCTTTTCTCCCTTGG PRIMER_RIGHT_1_SEQUENCE=AGACAATGAATGCCCCCTTT PRIMER_INTERNAL_1_SEQUENCE=TGTGGGACACTAGATCAGCAA PRIMER_LEFT_1=68,20 PRIMER_RIGHT_1=167,20 PRIMER_INTERNAL_1=94,21 PRIMER_LEFT_1_TM=60.040 PRIMER_RIGHT_1_TM=60.693 PRIMER_INTERNAL_1_TM=59.303 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=100 PRIMER_PAIR_2_PENALTY=0.733470 PRIMER_LEFT_2_PENALTY=0.040772 PRIMER_RIGHT_2_PENALTY=0.692698 PRIMER_INTERNAL_2_PENALTY=2.196745 PRIMER_LEFT_2_SEQUENCE=CCCTTGGTTAGAGTGTGGGA PRIMER_RIGHT_2_SEQUENCE=AGACAATGAATGCCCCCTTT PRIMER_INTERNAL_2_SEQUENCE=CATGAGTTTGAGAGGTAGCCC PRIMER_LEFT_2=81,20 PRIMER_RIGHT_2=167,20 PRIMER_INTERNAL_2=127,21 PRIMER_LEFT_2_TM=59.959 PRIMER_RIGHT_2_TM=60.693 PRIMER_INTERNAL_2_TM=58.803 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=87 PRIMER_PAIR_3_PENALTY=0.733470 PRIMER_LEFT_3_PENALTY=0.040772 PRIMER_RIGHT_3_PENALTY=0.692698 PRIMER_INTERNAL_3_PENALTY=2.196745 PRIMER_LEFT_3_SEQUENCE=TCCCTTGGTTAGAGTGTGGG PRIMER_RIGHT_3_SEQUENCE=AGACAATGAATGCCCCCTTT PRIMER_INTERNAL_3_SEQUENCE=CATGAGTTTGAGAGGTAGCCC PRIMER_LEFT_3=80,20 PRIMER_RIGHT_3=167,20 PRIMER_INTERNAL_3=127,21 PRIMER_LEFT_3_TM=59.959 PRIMER_RIGHT_3_TM=60.693 PRIMER_INTERNAL_3_TM=58.803 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=88 PRIMER_PAIR_4_PENALTY=0.893386 PRIMER_LEFT_4_PENALTY=0.040772 PRIMER_RIGHT_4_PENALTY=0.852615 PRIMER_INTERNAL_4_PENALTY=1.139192 PRIMER_LEFT_4_SEQUENCE=CCCTTGGTTAGAGTGTGGGA PRIMER_RIGHT_4_SEQUENCE=AGAGACAATGAATGCCCCCT PRIMER_INTERNAL_4_SEQUENCE=TGAGTTTGAGAGGTAGCCCAA PRIMER_LEFT_4=81,20 PRIMER_RIGHT_4=169,20 PRIMER_INTERNAL_4=129,21 PRIMER_LEFT_4_TM=59.959 PRIMER_RIGHT_4_TM=60.853 PRIMER_INTERNAL_4_TM=59.861 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=10.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=89 = SEQUENCE_ID=MH909 SEQUENCE_TEMPLATE=TCGACTCTAGAGGATCCCCCTAACTGCTCTAGTATNCTAGCCCTGAAAAACCACAAAGATGAGGATTAGCAGATGCTCAAGAAATATTTTTTGAATATGCTGGCTCTTATCTGCTCAGCAGTGTACAGATATGCTCTCACACTCGAGAAGCTACCAGAACAAACTGGTAGGCTCCCATTCTAGATGACATCTTTTCCCCATACTCTCAAAATGCAAGTTAACCCCCAACCCTGAGCAGAAGAAAGGAGAGGCCCCAGGTACCTGTCAATGAGGGAATCCCGCATGCTGGTGGCAATGGTGCTAGGCTGGGCTTCATTCAG SEQUENCE_INCLUDED_REGION=19,301 P3_COMMENT=CL is CTAGCCCTGAAAAACCACAA P3_COMMENT=CR is CTCATTGACAGGTACCTGGG P3_COMMENT=CM is GATGAGGATTAGCAGATGCTCA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.124966 PRIMER_LEFT_0_PENALTY=0.095713 PRIMER_RIGHT_0_PENALTY=0.029253 PRIMER_INTERNAL_0_PENALTY=1.025669 PRIMER_LEFT_0_SEQUENCE=AGCAGAAGAAAGGAGAGGCC PRIMER_RIGHT_0_SEQUENCE=CTGAATGAAGCCCAGCCTAG PRIMER_INTERNAL_0_SEQUENCE=CAGGTACCTGTCAATGAGGGA PRIMER_LEFT_0=233,20 PRIMER_RIGHT_0=319,20 PRIMER_INTERNAL_0=254,21 PRIMER_LEFT_0_TM=60.096 PRIMER_RIGHT_0_TM=59.971 PRIMER_INTERNAL_0_TM=59.974 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=87 PRIMER_PAIR_1_PENALTY=0.241412 PRIMER_LEFT_1_PENALTY=0.088413 PRIMER_RIGHT_1_PENALTY=0.152999 PRIMER_INTERNAL_1_PENALTY=2.385416 PRIMER_LEFT_1_SEQUENCE=GCCCTGAAAAACCACAAAGA PRIMER_RIGHT_1_SEQUENCE=TGAGCAGATAAGAGCCAGCA PRIMER_INTERNAL_1_SEQUENCE=TGAGGATTAGCAGATGCTCAAG PRIMER_LEFT_1=39,20 PRIMER_RIGHT_1=116,20 PRIMER_INTERNAL_1=59,22 PRIMER_LEFT_1_TM=60.088 PRIMER_RIGHT_1_TM=59.847 PRIMER_INTERNAL_1_TM=59.615 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=78 PRIMER_PAIR_2_PENALTY=0.241412 PRIMER_LEFT_2_PENALTY=0.088413 PRIMER_RIGHT_2_PENALTY=0.152999 PRIMER_INTERNAL_2_PENALTY=2.385416 PRIMER_LEFT_2_SEQUENCE=GCCCTGAAAAACCACAAAGA PRIMER_RIGHT_2_SEQUENCE=TGCTGAGCAGATAAGAGCCA PRIMER_INTERNAL_2_SEQUENCE=TGAGGATTAGCAGATGCTCAAG PRIMER_LEFT_2=39,20 PRIMER_RIGHT_2=119,20 PRIMER_INTERNAL_2=59,22 PRIMER_LEFT_2_TM=60.088 PRIMER_RIGHT_2_TM=59.847 PRIMER_INTERNAL_2_TM=59.615 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=0.395704 PRIMER_LEFT_3_PENALTY=0.366450 PRIMER_RIGHT_3_PENALTY=0.029253 PRIMER_INTERNAL_3_PENALTY=0.769217 PRIMER_LEFT_3_SEQUENCE=CCAACCCTGAGCAGAAGAAA PRIMER_RIGHT_3_SEQUENCE=CTGAATGAAGCCCAGCCTAG PRIMER_INTERNAL_3_SEQUENCE=CCCCAGGTACCTGTCAATGA PRIMER_LEFT_3=224,20 PRIMER_RIGHT_3=319,20 PRIMER_INTERNAL_3=251,20 PRIMER_LEFT_3_TM=60.366 PRIMER_RIGHT_3_TM=59.971 PRIMER_INTERNAL_3_TM=60.769 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=10.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=96 PRIMER_PAIR_4_PENALTY=0.503654 PRIMER_LEFT_4_PENALTY=0.407941 PRIMER_RIGHT_4_PENALTY=0.095713 PRIMER_INTERNAL_4_PENALTY=0.684590 PRIMER_LEFT_4_SEQUENCE=AACAAACTGGTAGGCTCCCA PRIMER_RIGHT_4_SEQUENCE=GGCCTCTCCTTTCTTCTGCT PRIMER_INTERNAL_4_SEQUENCE=AAATGCAAGTTAACCCCCAA PRIMER_LEFT_4=157,20 PRIMER_RIGHT_4=252,20 PRIMER_INTERNAL_4=208,20 PRIMER_LEFT_4_TM=59.592 PRIMER_RIGHT_4_TM=60.096 PRIMER_INTERNAL_4_TM=59.315 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=7.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=96 = SEQUENCE_ID=MH915 SEQUENCE_TEMPLATE=TTCATGCTNAGGTCGACTCTAGAGGATCCCCCTAAGATAAGAAGATACCACTGTAAAAAGGTTATTATTCCTAAAGAGGAACTAAACGTAGGCAAGGCCCCACTTTTCTGGAAGTGGCTTATCAGTGTGGATCCTAATAAGAACGAATGTNTAGCAATCACTTATATCCCAGACATGATTCTAAGTTCTCCATGTGCATTTACTCCTTTAATTCTCACAACACTCCTTGCAAGTGAGAAGTCTTATTATTTCCAAATTTCAGATACAGAAACTCAG SEQUENCE_INCLUDED_REGION=31,245 P3_COMMENT=CL is ACCACTGTAAAAAGGTTATTATTCC P3_COMMENT=CR is TGAGTTTCTGTATCTGAAATTTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.911049 PRIMER_LEFT_0_PENALTY=0.671597 PRIMER_RIGHT_0_PENALTY=3.239452 PRIMER_INTERNAL_0_PENALTY=4.439481 PRIMER_LEFT_0_SEQUENCE=CCACTTTTCTGGAAGTGGCT PRIMER_RIGHT_0_SEQUENCE=TGCACATGGAGAACTTAGAATCA PRIMER_INTERNAL_0_SEQUENCE=CAGTGTGGATCCTAATAAGAACGA PRIMER_LEFT_0=99,20 PRIMER_RIGHT_0=197,23 PRIMER_INTERNAL_0=122,24 PRIMER_LEFT_0_TM=59.328 PRIMER_RIGHT_0_TM=59.761 PRIMER_INTERNAL_0_TM=59.561 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=39.130 PRIMER_INTERNAL_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=99 PRIMER_PAIR_1_PENALTY=4.407680 PRIMER_LEFT_1_PENALTY=1.393460 PRIMER_RIGHT_1_PENALTY=3.014220 PRIMER_INTERNAL_1_PENALTY=4.236882 PRIMER_LEFT_1_SEQUENCE=GGAAGTGGCTTATCAGTGTGG PRIMER_RIGHT_1_SEQUENCE=AAGGAGTAAATGCACATGGAGAA PRIMER_INTERNAL_1_SEQUENCE=GCAATCACTTATATCCCAGACATG PRIMER_LEFT_1=109,21 PRIMER_RIGHT_1=207,23 PRIMER_INTERNAL_1=153,24 PRIMER_LEFT_1_TM=59.607 PRIMER_RIGHT_1_TM=60.014 PRIMER_INTERNAL_1_TM=59.763 PRIMER_LEFT_1_GC_PERCENT=52.381 PRIMER_RIGHT_1_GC_PERCENT=39.130 PRIMER_INTERNAL_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=4.407680 PRIMER_LEFT_2_PENALTY=1.393460 PRIMER_RIGHT_2_PENALTY=3.014220 PRIMER_INTERNAL_2_PENALTY=4.236882 PRIMER_LEFT_2_SEQUENCE=GGAAGTGGCTTATCAGTGTGG PRIMER_RIGHT_2_SEQUENCE=AAAGGAGTAAATGCACATGGAGA PRIMER_INTERNAL_2_SEQUENCE=GCAATCACTTATATCCCAGACATG PRIMER_LEFT_2=109,21 PRIMER_RIGHT_2=208,23 PRIMER_INTERNAL_2=153,24 PRIMER_LEFT_2_TM=59.607 PRIMER_RIGHT_2_TM=60.014 PRIMER_INTERNAL_2_TM=59.763 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=39.130 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=4.449466 PRIMER_LEFT_3_PENALTY=1.435246 PRIMER_RIGHT_3_PENALTY=3.014220 PRIMER_INTERNAL_3_PENALTY=4.236882 PRIMER_LEFT_3_SEQUENCE=TGGCTTATCAGTGTGGATCCT PRIMER_RIGHT_3_SEQUENCE=AAGGAGTAAATGCACATGGAGAA PRIMER_INTERNAL_3_SEQUENCE=GCAATCACTTATATCCCAGACATG PRIMER_LEFT_3=114,21 PRIMER_RIGHT_3=207,23 PRIMER_INTERNAL_3=153,24 PRIMER_LEFT_3_TM=59.565 PRIMER_RIGHT_3_TM=60.014 PRIMER_INTERNAL_3_TM=59.763 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=39.130 PRIMER_INTERNAL_3_GC_PERCENT=41.667 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.8000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=4.449466 PRIMER_LEFT_4_PENALTY=1.435246 PRIMER_RIGHT_4_PENALTY=3.014220 PRIMER_INTERNAL_4_PENALTY=4.236882 PRIMER_LEFT_4_SEQUENCE=TGGCTTATCAGTGTGGATCCT PRIMER_RIGHT_4_SEQUENCE=AAAGGAGTAAATGCACATGGAGA PRIMER_INTERNAL_4_SEQUENCE=GCAATCACTTATATCCCAGACATG PRIMER_LEFT_4=114,21 PRIMER_RIGHT_4=208,23 PRIMER_INTERNAL_4=153,24 PRIMER_LEFT_4_TM=59.565 PRIMER_RIGHT_4_TM=60.014 PRIMER_INTERNAL_4_TM=59.763 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=39.130 PRIMER_INTERNAL_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.8000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=95 = SEQUENCE_ID=MH917 SEQUENCE_TEMPLATE=TCATGCTCAGGTCGACTCTAGAGGATCCCCCTGGGGGTAAAGGTGGGAGGCAGTAGATGGATATAAAAGACCAGTGCTGCTCTCCCCCAAGTCCCCTCCTTTACCATTGCTTTCCTATGAATATTTTAAAGCAAAAGTAAAATAAGAATACCTAGGGCTTAATTTTTAAAGAGGGCACCTCATTTTATCCAAACCTCAGTAGGTACAGACCTGCTGCTTGGACAATATCACCTCAGCAGAGGCAACACTTCTGATGGCCACACTCCTAAATTTTAGTAAAAATACCTAAGAGGAAGCAATGAA SEQUENCE_INCLUDED_REGION=30,273 P3_COMMENT=CL is TGGGAGGCAGTAGATGGATA P3_COMMENT=CR is ATTTAGGAGTGTGGCCATCA P3_COMMENT=CM is AAAAGACCAGTGCTGCTCTC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.294966 PRIMER_LEFT_0_PENALTY=0.238079 PRIMER_RIGHT_0_PENALTY=0.056887 PRIMER_INTERNAL_0_PENALTY=0.125826 PRIMER_LEFT_0_SEQUENCE=GGGCACCTCATTTTATCCAA PRIMER_RIGHT_0_SEQUENCE=AGTGTTGCCTCTGCTGAGGT PRIMER_INTERNAL_0_SEQUENCE=AGACCTGCTGCTTGGACAAT PRIMER_LEFT_0=172,20 PRIMER_RIGHT_0=248,20 PRIMER_INTERNAL_0=206,20 PRIMER_LEFT_0_TM=59.762 PRIMER_RIGHT_0_TM=60.057 PRIMER_INTERNAL_0_TM=59.874 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 PRIMER_PAIR_1_PENALTY=0.357888 PRIMER_LEFT_1_PENALTY=0.238079 PRIMER_RIGHT_1_PENALTY=0.119809 PRIMER_INTERNAL_1_PENALTY=0.125826 PRIMER_LEFT_1_SEQUENCE=GGGCACCTCATTTTATCCAA PRIMER_RIGHT_1_SEQUENCE=GGAGTGTGGCCATCAGAAGT PRIMER_INTERNAL_1_SEQUENCE=AGACCTGCTGCTTGGACAAT PRIMER_LEFT_1=172,20 PRIMER_RIGHT_1=265,20 PRIMER_INTERNAL_1=206,20 PRIMER_LEFT_1_TM=59.762 PRIMER_RIGHT_1_TM=60.120 PRIMER_INTERNAL_1_TM=59.874 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=94 PRIMER_PAIR_2_PENALTY=0.468489 PRIMER_LEFT_2_PENALTY=0.238079 PRIMER_RIGHT_2_PENALTY=0.230410 PRIMER_INTERNAL_2_PENALTY=0.125826 PRIMER_LEFT_2_SEQUENCE=GGGCACCTCATTTTATCCAA PRIMER_RIGHT_2_SEQUENCE=CAGAAGTGTTGCCTCTGCTG PRIMER_INTERNAL_2_SEQUENCE=AGACCTGCTGCTTGGACAAT PRIMER_LEFT_2=172,20 PRIMER_RIGHT_2=252,20 PRIMER_INTERNAL_2=206,20 PRIMER_LEFT_2_TM=59.762 PRIMER_RIGHT_2_TM=59.770 PRIMER_INTERNAL_2_TM=59.874 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=81 PRIMER_PAIR_3_PENALTY=0.498835 PRIMER_LEFT_3_PENALTY=0.238079 PRIMER_RIGHT_3_PENALTY=0.260757 PRIMER_INTERNAL_3_PENALTY=0.125826 PRIMER_LEFT_3_SEQUENCE=GGGCACCTCATTTTATCCAA PRIMER_RIGHT_3_SEQUENCE=AGGAGTGTGGCCATCAGAAG PRIMER_INTERNAL_3_SEQUENCE=AGACCTGCTGCTTGGACAAT PRIMER_LEFT_3=172,20 PRIMER_RIGHT_3=266,20 PRIMER_INTERNAL_3=206,20 PRIMER_LEFT_3_TM=59.762 PRIMER_RIGHT_3_TM=60.261 PRIMER_INTERNAL_3_TM=59.874 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=95 PRIMER_PAIR_4_PENALTY=0.549862 PRIMER_LEFT_4_PENALTY=0.311784 PRIMER_RIGHT_4_PENALTY=0.238079 PRIMER_INTERNAL_4_PENALTY=8.558113 PRIMER_LEFT_4_SEQUENCE=CCCCTCCTTTACCATTGCTT PRIMER_RIGHT_4_SEQUENCE=TTGGATAAAATGAGGTGCCC PRIMER_INTERNAL_4_SEQUENCE=CAAAAGTAAAATAAGAATACCTAGGGC PRIMER_LEFT_4=92,20 PRIMER_RIGHT_4=191,20 PRIMER_INTERNAL_4=131,27 PRIMER_LEFT_4_TM=60.312 PRIMER_RIGHT_4_TM=59.762 PRIMER_INTERNAL_4_TM=58.442 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=11.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=100 = SEQUENCE_ID=MH918 SEQUENCE_TEMPLATE=ATGCTCAACCACTCTCCTCTAACAGCCCTGTGAACTTCCTCTGGCTTAGAATACATTGACCTGACTCTGTTGTAATTGCCGGGTTTTTNATTTGTCTTCTCCATTAGACCTCGGTTTCCAAATCTCGGCACAATTGACATCTTGGGAAAACTCACTCTTGGCTTGGGTGGGTGATAAGGGACTGTCCTGTGCATTT SEQUENCE_INCLUDED_REGION=0,196 P3_COMMENT=CL is TCTCCTCTAACAGCCCTGTG P3_COMMENT=CR is ACAGGACAGTCCCTTATCACC P3_COMMENT=CM is ACTTCCTCTGGCTTAGAATACATTG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.239244 PRIMER_LEFT_0_PENALTY=0.006945 PRIMER_RIGHT_0_PENALTY=0.232299 PRIMER_INTERNAL_0_PENALTY=0.074208 PRIMER_LEFT_0_SEQUENCE=CTCTGTTGTAATTGCCGGGT PRIMER_RIGHT_0_SEQUENCE=GCCAAGAGTGAGTTTTCCCA PRIMER_INTERNAL_0_SEQUENCE=CCATTAGACCTCGGTTTCCA PRIMER_LEFT_0=64,20 PRIMER_RIGHT_0=161,20 PRIMER_INTERNAL_0=100,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.232 PRIMER_INTERNAL_0_TM=59.926 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=11.1000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=98 PRIMER_PAIR_1_PENALTY=0.239244 PRIMER_LEFT_1_PENALTY=0.006945 PRIMER_RIGHT_1_PENALTY=0.232299 PRIMER_INTERNAL_1_PENALTY=0.074208 PRIMER_LEFT_1_SEQUENCE=ACTCTGTTGTAATTGCCGGG PRIMER_RIGHT_1_SEQUENCE=GCCAAGAGTGAGTTTTCCCA PRIMER_INTERNAL_1_SEQUENCE=CCATTAGACCTCGGTTTCCA PRIMER_LEFT_1=63,20 PRIMER_RIGHT_1=161,20 PRIMER_INTERNAL_1=100,20 PRIMER_LEFT_1_TM=59.993 PRIMER_RIGHT_1_TM=60.232 PRIMER_INTERNAL_1_TM=59.926 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=12.9000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=0.295053 PRIMER_LEFT_2_PENALTY=0.074208 PRIMER_RIGHT_2_PENALTY=0.220845 PRIMER_INTERNAL_2_PENALTY=0.232299 PRIMER_LEFT_2_SEQUENCE=CCATTAGACCTCGGTTTCCA PRIMER_RIGHT_2_SEQUENCE=GGACAGTCCCTTATCACCCA PRIMER_INTERNAL_2_SEQUENCE=TGGGAAAACTCACTCTTGGC PRIMER_LEFT_2=100,20 PRIMER_RIGHT_2=186,20 PRIMER_INTERNAL_2=142,20 PRIMER_LEFT_2_TM=59.926 PRIMER_RIGHT_2_TM=59.779 PRIMER_INTERNAL_2_TM=60.232 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=87 PRIMER_PAIR_3_PENALTY=0.371428 PRIMER_LEFT_3_PENALTY=0.139129 PRIMER_RIGHT_3_PENALTY=0.232299 PRIMER_INTERNAL_3_PENALTY=0.074208 PRIMER_LEFT_3_SEQUENCE=CTGTTGTAATTGCCGGGTTT PRIMER_RIGHT_3_SEQUENCE=GCCAAGAGTGAGTTTTCCCA PRIMER_INTERNAL_3_SEQUENCE=CCATTAGACCTCGGTTTCCA PRIMER_LEFT_3=66,20 PRIMER_RIGHT_3=161,20 PRIMER_INTERNAL_3=100,20 PRIMER_LEFT_3_TM=59.861 PRIMER_RIGHT_3_TM=60.232 PRIMER_INTERNAL_3_TM=59.926 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=96 PRIMER_PAIR_4_PENALTY=0.455048 PRIMER_LEFT_4_PENALTY=0.222749 PRIMER_RIGHT_4_PENALTY=0.232299 PRIMER_INTERNAL_4_PENALTY=0.074208 PRIMER_LEFT_4_SEQUENCE=TGTTGTAATTGCCGGGTTTT PRIMER_RIGHT_4_SEQUENCE=GCCAAGAGTGAGTTTTCCCA PRIMER_INTERNAL_4_SEQUENCE=CCATTAGACCTCGGTTTCCA PRIMER_LEFT_4=67,20 PRIMER_RIGHT_4=161,20 PRIMER_INTERNAL_4=100,20 PRIMER_LEFT_4_TM=60.223 PRIMER_RIGHT_4_TM=60.232 PRIMER_INTERNAL_4_TM=59.926 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=95 = SEQUENCE_ID=MH919 SEQUENCE_TEMPLATE=NNTTCANTNCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGGTAGCCTTTAAACTAGTGTAGCACTGAAGTATGTTTGCTGTGTTTAGTTTANTATAATTGAATTTTTAACTTAAAATCTTACTGTCTTATTTGGAAGAAGCACCAGTTCAAAGCACATAGGTCTACGTTGATAAATGAATAACGCTGATGTTAAGCACATTTATTATGAATGTNATTTCTCAAGATGCACCTGCTGCAGCGGAGAAGAATCAGTCCTGGTCAAAGTGAGCAGTAGGTAGAG SEQUENCE_INCLUDED_REGION=35,247 P3_COMMENT=CL is AGTGTAGCACTGAAGTATGTTTGC P3_COMMENT=CR is AGGACTGATTCTTCTCCGCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.645925 PRIMER_LEFT_0_PENALTY=0.509923 PRIMER_RIGHT_0_PENALTY=0.136001 PRIMER_INTERNAL_0_PENALTY=3.163489 PRIMER_LEFT_0_SEQUENCE=AAGCACCAGTTCAAAGCACA PRIMER_RIGHT_0_SEQUENCE=CAGCAGGTGCATCTTGAGAA PRIMER_INTERNAL_0_SEQUENCE=AACGCTGATGTTAAGCACATTT PRIMER_LEFT_0=138,20 PRIMER_RIGHT_0=236,20 PRIMER_INTERNAL_0=181,22 PRIMER_LEFT_0_TM=59.490 PRIMER_RIGHT_0_TM=60.136 PRIMER_INTERNAL_0_TM=58.837 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=36.364 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=99 PRIMER_PAIR_1_PENALTY=1.341407 PRIMER_LEFT_1_PENALTY=1.205406 PRIMER_RIGHT_1_PENALTY=0.136001 PRIMER_INTERNAL_1_PENALTY=3.163489 PRIMER_LEFT_1_SEQUENCE=AAGCACCAGTTCAAAGCACAT PRIMER_RIGHT_1_SEQUENCE=CAGCAGGTGCATCTTGAGAA PRIMER_INTERNAL_1_SEQUENCE=AACGCTGATGTTAAGCACATTT PRIMER_LEFT_1=138,21 PRIMER_RIGHT_1=236,20 PRIMER_INTERNAL_1=181,22 PRIMER_LEFT_1_TM=59.795 PRIMER_RIGHT_1_TM=60.136 PRIMER_INTERNAL_1_TM=58.837 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=36.364 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=99 PRIMER_PAIR_2_PENALTY=1.637950 PRIMER_LEFT_2_PENALTY=1.501949 PRIMER_RIGHT_2_PENALTY=0.136001 PRIMER_INTERNAL_2_PENALTY=3.163489 PRIMER_LEFT_2_SEQUENCE=GAAGCACCAGTTCAAAGCAC PRIMER_RIGHT_2_SEQUENCE=CAGCAGGTGCATCTTGAGAA PRIMER_INTERNAL_2_SEQUENCE=AACGCTGATGTTAAGCACATTT PRIMER_LEFT_2=137,20 PRIMER_RIGHT_2=236,20 PRIMER_INTERNAL_2=181,22 PRIMER_LEFT_2_TM=58.498 PRIMER_RIGHT_2_TM=60.136 PRIMER_INTERNAL_2_TM=58.837 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=36.364 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=100 PRIMER_PAIR_3_PENALTY=1.764958 PRIMER_LEFT_3_PENALTY=1.628957 PRIMER_RIGHT_3_PENALTY=0.136001 PRIMER_INTERNAL_3_PENALTY=3.163489 PRIMER_LEFT_3_SEQUENCE=AGCACCAGTTCAAAGCACAT PRIMER_RIGHT_3_SEQUENCE=CAGCAGGTGCATCTTGAGAA PRIMER_INTERNAL_3_SEQUENCE=AACGCTGATGTTAAGCACATTT PRIMER_LEFT_3=139,20 PRIMER_RIGHT_3=236,20 PRIMER_INTERNAL_3=181,22 PRIMER_LEFT_3_TM=58.371 PRIMER_RIGHT_3_TM=60.136 PRIMER_INTERNAL_3_TM=58.837 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=36.364 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=98 PRIMER_PAIR_4_PENALTY=1.912469 PRIMER_LEFT_4_PENALTY=0.509923 PRIMER_RIGHT_4_PENALTY=1.402545 PRIMER_INTERNAL_4_PENALTY=3.163489 PRIMER_LEFT_4_SEQUENCE=AAGCACCAGTTCAAAGCACA PRIMER_RIGHT_4_SEQUENCE=AGCAGGTGCATCTTGAGAAA PRIMER_INTERNAL_4_SEQUENCE=AACGCTGATGTTAAGCACATTT PRIMER_LEFT_4=138,20 PRIMER_RIGHT_4=235,20 PRIMER_INTERNAL_4=181,22 PRIMER_LEFT_4_TM=59.490 PRIMER_RIGHT_4_TM=58.597 PRIMER_INTERNAL_4_TM=58.837 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=36.364 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=98 = SEQUENCE_ID=MH936 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTCAGCCTTTAGCCCCTCTCCCTTCACTGGAGGTTGGGGCTGGCGGGAGGGGTGGGCCTGAAACTTCCAACCCTCTAATTAGAGGGTACCGNGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTNAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGT SEQUENCE_INCLUDED_REGION=36,82 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.255193 PRIMER_LEFT_0_PENALTY=1.483058 PRIMER_RIGHT_0_PENALTY=1.772136 PRIMER_INTERNAL_0_PENALTY=2.985442 PRIMER_LEFT_0_SEQUENCE=TCAGCCTTTAGCCCCTCTC PRIMER_RIGHT_0_SEQUENCE=AGAGGGTTGGAAGTTTCAGG PRIMER_INTERNAL_0_SEQUENCE=CTTCACTGGAGGTTGGGG PRIMER_LEFT_0=36,19 PRIMER_RIGHT_0=110,20 PRIMER_INTERNAL_0=56,18 PRIMER_LEFT_0_TM=59.517 PRIMER_RIGHT_0_TM=58.228 PRIMER_INTERNAL_0_TM=59.015 PRIMER_LEFT_0_GC_PERCENT=57.895 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=3.856833 PRIMER_LEFT_1_PENALTY=1.483058 PRIMER_RIGHT_1_PENALTY=2.373775 PRIMER_INTERNAL_1_PENALTY=2.985442 PRIMER_LEFT_1_SEQUENCE=TCAGCCTTTAGCCCCTCTC PRIMER_RIGHT_1_SEQUENCE=TTAGAGGGTTGGAAGTTTCAGG PRIMER_INTERNAL_1_SEQUENCE=CTTCACTGGAGGTTGGGG PRIMER_LEFT_1=36,19 PRIMER_RIGHT_1=112,22 PRIMER_INTERNAL_1=56,18 PRIMER_LEFT_1_TM=59.517 PRIMER_RIGHT_1_TM=59.626 PRIMER_INTERNAL_1_TM=59.015 PRIMER_LEFT_1_GC_PERCENT=57.895 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_INTERNAL_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=4.178092 PRIMER_LEFT_2_PENALTY=1.483058 PRIMER_RIGHT_2_PENALTY=2.695034 PRIMER_INTERNAL_2_PENALTY=2.985442 PRIMER_LEFT_2_SEQUENCE=TCAGCCTTTAGCCCCTCTC PRIMER_RIGHT_2_SEQUENCE=TAGAGGGTTGGAAGTTTCAGG PRIMER_INTERNAL_2_SEQUENCE=CTTCACTGGAGGTTGGGG PRIMER_LEFT_2=36,19 PRIMER_RIGHT_2=111,21 PRIMER_INTERNAL_2=56,18 PRIMER_LEFT_2_TM=59.517 PRIMER_RIGHT_2_TM=58.305 PRIMER_INTERNAL_2_TM=59.015 PRIMER_LEFT_2_GC_PERCENT=57.895 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=76 PRIMER_PAIR_3_PENALTY=4.352208 PRIMER_LEFT_3_PENALTY=1.483058 PRIMER_RIGHT_3_PENALTY=2.869150 PRIMER_INTERNAL_3_PENALTY=2.985442 PRIMER_LEFT_3_SEQUENCE=TCAGCCTTTAGCCCCTCTC PRIMER_RIGHT_3_SEQUENCE=AGAGGGTTGGAAGTTTCAGGC PRIMER_INTERNAL_3_SEQUENCE=CTTCACTGGAGGTTGGGG PRIMER_LEFT_3=36,19 PRIMER_RIGHT_3=110,21 PRIMER_INTERNAL_3=56,18 PRIMER_LEFT_3_TM=59.517 PRIMER_RIGHT_3_TM=61.869 PRIMER_INTERNAL_3_TM=59.015 PRIMER_LEFT_3_GC_PERCENT=57.895 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_INTERNAL_3_GC_PERCENT=61.111 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=4.594966 PRIMER_LEFT_4_PENALTY=1.483058 PRIMER_RIGHT_4_PENALTY=3.111908 PRIMER_INTERNAL_4_PENALTY=2.985442 PRIMER_LEFT_4_SEQUENCE=TCAGCCTTTAGCCCCTCTC PRIMER_RIGHT_4_SEQUENCE=ATTAGAGGGTTGGAAGTTTCAGG PRIMER_INTERNAL_4_SEQUENCE=CTTCACTGGAGGTTGGGG PRIMER_LEFT_4=36,19 PRIMER_RIGHT_4=113,23 PRIMER_INTERNAL_4=56,18 PRIMER_LEFT_4_TM=59.517 PRIMER_RIGHT_4_TM=59.888 PRIMER_INTERNAL_4_TM=59.015 PRIMER_LEFT_4_GC_PERCENT=57.895 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_INTERNAL_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=78 = SEQUENCE_ID=MH952 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGCTGTTCAGGTACCCCTTCCAGAGAAGCCAGGAGCACCCGGCGTCCCANACAAGTAAGTAAGCGCTGGGCGGGGGCCTGGCGCTCGCNGGGGCCGCCTCATTCCGGGGTCGCCTCATTCCAGGGCCGCCTCGCGAG SEQUENCE_INCLUDED_REGION=36,137 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=MH958 SEQUENCE_TEMPLATE=TTGCTCTGCCTGGAGCATCCTCTCTGACGTGGCTCTGTGATTCACTTTGCGCCCCTCCTCAGGTCTCTTCTTCTCCGTTACCTCCCTAGACAGGTTTTCCCTCTGCCCTGCTCTCAATCACTCACTAGTGTTATTTTTCTTCATGCCATTTGTATTCTCTCCTGTCTTCAATTGCTTATTGCTTATGTGTTTATTGTTACCCACCCCAACTGGAATGAAAGATCCATGCAGACAAGGACTTTGTTTTTGCTCACTGCAGTGACTCCACCACCTAGAACAGTGCTTGGTG SEQUENCE_INCLUDED_REGION=0,289 P3_COMMENT=CL is CTTCTTCTCCGTTACCTCCC P3_COMMENT=CR is AGCACTGTTCTAGGTGGTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.683573 PRIMER_LEFT_0_PENALTY=0.055279 PRIMER_RIGHT_0_PENALTY=0.628294 PRIMER_INTERNAL_0_PENALTY=0.199123 PRIMER_LEFT_0_SEQUENCE=CCTGGAGCATCCTCTCTGAC PRIMER_RIGHT_0_SEQUENCE=GGCAGAGGGAAAACCTGTCT PRIMER_INTERNAL_0_SEQUENCE=CCCCTCCTCAGGTCTCTTCT PRIMER_LEFT_0=8,20 PRIMER_RIGHT_0=106,20 PRIMER_INTERNAL_0=51,20 PRIMER_LEFT_0_TM=59.945 PRIMER_RIGHT_0_TM=60.628 PRIMER_INTERNAL_0_TM=59.801 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=99 PRIMER_PAIR_1_PENALTY=0.740888 PRIMER_LEFT_1_PENALTY=0.431486 PRIMER_RIGHT_1_PENALTY=0.309402 PRIMER_INTERNAL_1_PENALTY=0.711868 PRIMER_LEFT_1_SEQUENCE=ATTGTTACCCACCCCAACTG PRIMER_RIGHT_1_SEQUENCE=TAGGTGGTGGAGTCACTGCA PRIMER_INTERNAL_1_SEQUENCE=CCATGCAGACAAGGACTTTG PRIMER_LEFT_1=192,20 PRIMER_RIGHT_1=273,20 PRIMER_INTERNAL_1=223,20 PRIMER_LEFT_1_TM=59.569 PRIMER_RIGHT_1_TM=60.309 PRIMER_INTERNAL_1_TM=59.288 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=0.871517 PRIMER_LEFT_2_PENALTY=0.243223 PRIMER_RIGHT_2_PENALTY=0.628294 PRIMER_INTERNAL_2_PENALTY=0.199123 PRIMER_LEFT_2_SEQUENCE=CTCTCTGACGTGGCTCTGTG PRIMER_RIGHT_2_SEQUENCE=GGCAGAGGGAAAACCTGTCT PRIMER_INTERNAL_2_SEQUENCE=CCCCTCCTCAGGTCTCTTCT PRIMER_LEFT_2=19,20 PRIMER_RIGHT_2=106,20 PRIMER_INTERNAL_2=51,20 PRIMER_LEFT_2_TM=59.757 PRIMER_RIGHT_2_TM=60.628 PRIMER_INTERNAL_2_TM=59.801 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=88 PRIMER_PAIR_3_PENALTY=0.958438 PRIMER_LEFT_3_PENALTY=0.403673 PRIMER_RIGHT_3_PENALTY=0.554765 PRIMER_INTERNAL_3_PENALTY=1.301100 PRIMER_LEFT_3_SEQUENCE=CTCTGTGATTCACTTTGCGC PRIMER_RIGHT_3_SEQUENCE=AGTGATTGAGAGCAGGGCAG PRIMER_INTERNAL_3_SEQUENCE=TCTTCTTCTCCGTTACCTCCC PRIMER_LEFT_3=32,20 PRIMER_RIGHT_3=121,20 PRIMER_INTERNAL_3=65,21 PRIMER_LEFT_3_TM=59.596 PRIMER_RIGHT_3_TM=60.555 PRIMER_INTERNAL_3_TM=59.699 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=11.7000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=0.958438 PRIMER_LEFT_4_PENALTY=0.403673 PRIMER_RIGHT_4_PENALTY=0.554765 PRIMER_INTERNAL_4_PENALTY=0.199123 PRIMER_LEFT_4_SEQUENCE=GCTCTGTGATTCACTTTGCG PRIMER_RIGHT_4_SEQUENCE=AGTGATTGAGAGCAGGGCAG PRIMER_INTERNAL_4_SEQUENCE=CCCCTCCTCAGGTCTCTTCT PRIMER_LEFT_4=31,20 PRIMER_RIGHT_4=121,20 PRIMER_INTERNAL_4=51,20 PRIMER_LEFT_4_TM=59.596 PRIMER_RIGHT_4_TM=60.555 PRIMER_INTERNAL_4_TM=59.801 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=10.5000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=91 = SEQUENCE_ID=MH959 SEQUENCE_TEMPLATE=TGCATGCCTNCAGGTCGACTCTAGAGGATCCCCCTTTTAAACAAACCTGCAGTAGAAGCAAGCCCTCTCCAGGAAATCCCAATCTCTTTCCAAACAGGGACAGCAAAGGTTCTCTCTGTTGTGGCTGTTTTTCCCTCTCTTCTGGGAATTGGTTTGCCTTCTGAGAGCATCCCAGGAAGGGGAGGTGCGCCCCAAATTGGCGAGGCTCTGCAAATACCAAAGGCTCTTCTTTGGAAGAAGACTCCTCCTTTGGACGTGAGGCGCCCCTACCAGAGCGCCCGCNTACAGAAACGGAAACCACTAGCTACCTGGGAGG SEQUENCE_INCLUDED_REGION=33,283 P3_COMMENT=CL is AGTAGAAGCAAGCCCTCTCC P3_COMMENT=CR is ACGTCCAAAGGAGGAGTCTT P3_COMMENT=CM is GGAAATCCCAATCTCTTTCC P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.199972 PRIMER_LEFT_0_PENALTY=0.051345 PRIMER_RIGHT_0_PENALTY=0.148627 PRIMER_INTERNAL_0_PENALTY=2.672380 PRIMER_LEFT_0_SEQUENCE=GGAATTGGTTTGCCTTCTGA PRIMER_RIGHT_0_SEQUENCE=GCCTTTGGTATTTGCAGAGC PRIMER_INTERNAL_0_SEQUENCE=ATCCCAGGAAGGGGAGGT PRIMER_LEFT_0=144,20 PRIMER_RIGHT_0=223,20 PRIMER_INTERNAL_0=168,18 PRIMER_LEFT_0_TM=60.051 PRIMER_RIGHT_0_TM=59.851 PRIMER_INTERNAL_0_TM=60.672 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=0.199972 PRIMER_LEFT_1_PENALTY=0.051345 PRIMER_RIGHT_1_PENALTY=0.148627 PRIMER_INTERNAL_1_PENALTY=0.487936 PRIMER_LEFT_1_SEQUENCE=TCTTCTGGGAATTGGTTTGC PRIMER_RIGHT_1_SEQUENCE=GCCTTTGGTATTTGCAGAGC PRIMER_INTERNAL_1_SEQUENCE=TCTGAGAGCATCCCAGGAAG PRIMER_LEFT_1=137,20 PRIMER_RIGHT_1=223,20 PRIMER_INTERNAL_1=159,20 PRIMER_LEFT_1_TM=60.051 PRIMER_RIGHT_1_TM=59.851 PRIMER_INTERNAL_1_TM=60.488 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=87 PRIMER_PAIR_2_PENALTY=0.283644 PRIMER_LEFT_2_PENALTY=0.232299 PRIMER_RIGHT_2_PENALTY=0.051345 PRIMER_INTERNAL_2_PENALTY=0.232299 PRIMER_LEFT_2_SEQUENCE=TCTTTCCAAACAGGGACAGC PRIMER_RIGHT_2_SEQUENCE=TCAGAAGGCAAACCAATTCC PRIMER_INTERNAL_2_SEQUENCE=TGTGGCTGTTTTTCCCTCTC PRIMER_LEFT_2=84,20 PRIMER_RIGHT_2=163,20 PRIMER_INTERNAL_2=119,20 PRIMER_LEFT_2_TM=60.232 PRIMER_RIGHT_2_TM=60.051 PRIMER_INTERNAL_2_TM=60.232 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=8.1000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=80 PRIMER_PAIR_3_PENALTY=0.314950 PRIMER_LEFT_3_PENALTY=0.263605 PRIMER_RIGHT_3_PENALTY=0.051345 PRIMER_INTERNAL_3_PENALTY=0.148509 PRIMER_LEFT_3_SEQUENCE=CCTCTCCAGGAAATCCCAAT PRIMER_RIGHT_3_SEQUENCE=GCAAACCAATTCCCAGAAGA PRIMER_INTERNAL_3_SEQUENCE=CAAACAGGGACAGCAAAGGT PRIMER_LEFT_3=63,20 PRIMER_RIGHT_3=156,20 PRIMER_INTERNAL_3=90,20 PRIMER_LEFT_3_TM=60.264 PRIMER_RIGHT_3_TM=60.051 PRIMER_INTERNAL_3_TM=60.149 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=94 PRIMER_PAIR_4_PENALTY=0.343759 PRIMER_LEFT_4_PENALTY=0.051345 PRIMER_RIGHT_4_PENALTY=0.292414 PRIMER_INTERNAL_4_PENALTY=2.672380 PRIMER_LEFT_4_SEQUENCE=GGAATTGGTTTGCCTTCTGA PRIMER_RIGHT_4_SEQUENCE=CCTTTGGTATTTGCAGAGCC PRIMER_INTERNAL_4_SEQUENCE=ATCCCAGGAAGGGGAGGT PRIMER_LEFT_4=144,20 PRIMER_RIGHT_4=222,20 PRIMER_INTERNAL_4=168,18 PRIMER_LEFT_4_TM=60.051 PRIMER_RIGHT_4_TM=59.708 PRIMER_INTERNAL_4_TM=60.672 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH963 SEQUENCE_TEMPLATE=TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTATTCTTTTCATGCTGTATCATTTTTAATAACTCAGTTAAAAGTTGTCTTTTTAGAATGCCTTCTTATGGTNTTATGATCTGGCCTCACTTCGCGAATACTAATTGAAAGAGCCAAGATATGGTTACAGAAGGTCCAGATGAATGGAGATGTGTCACTAGCAGAACAGAGTTGACTTTCTCCATGGGAATCTATCCCTGAAACAAAGGGACTGTCACTTCAATGACCCATGAGCCTCAGTTAGTCACTAAGCAGACATATTTGAGAAACATGCCTTATCAATTACTTCAGGAAATAAGTATTTTTAAAAAATGAAAATTTATTAACT SEQUENCE_INCLUDED_REGION=34,328 P3_COMMENT=CL is GCCTCACTTCGCGAATACTA P3_COMMENT=CR is TATTTCCTGAAGTAATTGATAAGGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.138382 PRIMER_LEFT_0_PENALTY=1.057653 PRIMER_RIGHT_0_PENALTY=0.080729 PRIMER_INTERNAL_0_PENALTY=1.873692 PRIMER_LEFT_0_SEQUENCE=CAGAAGGTCCAGATGAATGGA PRIMER_RIGHT_0_SEQUENCE=CAGTCCCTTTGTTTCAGGGA PRIMER_INTERNAL_0_SEQUENCE=GAGTTGACTTTCTCCATGGGA PRIMER_LEFT_0=161,21 PRIMER_RIGHT_0=247,20 PRIMER_INTERNAL_0=202,21 PRIMER_LEFT_0_TM=60.058 PRIMER_RIGHT_0_TM=60.081 PRIMER_INTERNAL_0_TM=59.126 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=8.1000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=87 PRIMER_PAIR_1_PENALTY=2.001567 PRIMER_LEFT_1_PENALTY=1.920838 PRIMER_RIGHT_1_PENALTY=0.080729 PRIMER_INTERNAL_1_PENALTY=1.873692 PRIMER_LEFT_1_SEQUENCE=GAAGGTCCAGATGAATGGAGA PRIMER_RIGHT_1_SEQUENCE=CAGTCCCTTTGTTTCAGGGA PRIMER_INTERNAL_1_SEQUENCE=GAGTTGACTTTCTCCATGGGA PRIMER_LEFT_1=163,21 PRIMER_RIGHT_1=247,20 PRIMER_INTERNAL_1=202,21 PRIMER_LEFT_1_TM=59.079 PRIMER_RIGHT_1_TM=60.081 PRIMER_INTERNAL_1_TM=59.126 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=85 PRIMER_PAIR_2_PENALTY=2.009392 PRIMER_LEFT_2_PENALTY=1.928663 PRIMER_RIGHT_2_PENALTY=0.080729 PRIMER_INTERNAL_2_PENALTY=1.873692 PRIMER_LEFT_2_SEQUENCE=CAGAAGGTCCAGATGAATGG PRIMER_RIGHT_2_SEQUENCE=CAGTCCCTTTGTTTCAGGGA PRIMER_INTERNAL_2_SEQUENCE=GAGTTGACTTTCTCCATGGGA PRIMER_LEFT_2=161,20 PRIMER_RIGHT_2=247,20 PRIMER_INTERNAL_2=202,21 PRIMER_LEFT_2_TM=58.071 PRIMER_RIGHT_2_TM=60.081 PRIMER_INTERNAL_2_TM=59.126 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=87 PRIMER_PAIR_3_PENALTY=2.021147 PRIMER_LEFT_3_PENALTY=1.057653 PRIMER_RIGHT_3_PENALTY=0.963493 PRIMER_INTERNAL_3_PENALTY=1.873692 PRIMER_LEFT_3_SEQUENCE=CAGAAGGTCCAGATGAATGGA PRIMER_RIGHT_3_SEQUENCE=ACAGTCCCTTTGTTTCAGGG PRIMER_INTERNAL_3_SEQUENCE=GAGTTGACTTTCTCCATGGGA PRIMER_LEFT_3=161,21 PRIMER_RIGHT_3=248,20 PRIMER_INTERNAL_3=202,21 PRIMER_LEFT_3_TM=60.058 PRIMER_RIGHT_3_TM=59.037 PRIMER_INTERNAL_3_TM=59.126 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=88 PRIMER_PAIR_4_PENALTY=2.077407 PRIMER_LEFT_4_PENALTY=1.996678 PRIMER_RIGHT_4_PENALTY=0.080729 PRIMER_INTERNAL_4_PENALTY=1.873692 PRIMER_LEFT_4_SEQUENCE=ACAGAAGGTCCAGATGAATGG PRIMER_RIGHT_4_SEQUENCE=CAGTCCCTTTGTTTCAGGGA PRIMER_INTERNAL_4_SEQUENCE=GAGTTGACTTTCTCCATGGGA PRIMER_LEFT_4=160,21 PRIMER_RIGHT_4=247,20 PRIMER_INTERNAL_4=202,21 PRIMER_LEFT_4_TM=59.003 PRIMER_RIGHT_4_TM=60.081 PRIMER_INTERNAL_4_TM=59.126 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=88 = SEQUENCE_ID=MH968 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCACATATATGAAGTNAAGATGGCAGGTGCCAACCTGTCCACTTCTTGGCAGAAAATGATCTCAGTCACCAAGGGAAGGGGAGGCTCAGAGCAACTCTTCCTGGGAGTGTCCTTAGGACAATGACTGTTGGCTCACCTTGAAGTACACAGTGCCCCGCTCTTTCACTATGGTGCTCTGTTCCAG SEQUENCE_INCLUDED_REGION=36,185 P3_COMMENT=CL is GTCCACTTCTTGGCAGAAAA P3_COMMENT=CR is AGCACCATAGTGAAAGAGCG P3_COMMENT=CM is GATCTCAGTCACCAAGGGAA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.509870 PRIMER_LEFT_0_PENALTY=0.158723 PRIMER_RIGHT_0_PENALTY=0.351148 PRIMER_INTERNAL_0_PENALTY=0.229181 PRIMER_LEFT_0_SEQUENCE=GTGCCAACCTGTCCACTTCT PRIMER_RIGHT_0_SEQUENCE=TCCTAAGGACACTCCCAGGA PRIMER_INTERNAL_0_SEQUENCE=TCTCAGTCACCAAGGGAAGG PRIMER_LEFT_0=63,20 PRIMER_RIGHT_0=154,20 PRIMER_INTERNAL_0=96,20 PRIMER_LEFT_0_TM=60.159 PRIMER_RIGHT_0_TM=59.649 PRIMER_INTERNAL_0_TM=60.229 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=92 PRIMER_PAIR_1_PENALTY=0.547116 PRIMER_LEFT_1_PENALTY=0.229181 PRIMER_RIGHT_1_PENALTY=0.317935 PRIMER_INTERNAL_1_PENALTY=0.523038 PRIMER_LEFT_1_SEQUENCE=TCTCAGTCACCAAGGGAAGG PRIMER_RIGHT_1_SEQUENCE=TGAGCCAACAGTCATTGTCC PRIMER_INTERNAL_1_SEQUENCE=CAGAGCAACTCTTCCTGGGA PRIMER_LEFT_1=96,20 PRIMER_RIGHT_1=171,20 PRIMER_INTERNAL_1=123,20 PRIMER_LEFT_1_TM=60.229 PRIMER_RIGHT_1_TM=59.682 PRIMER_INTERNAL_1_TM=60.523 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=76 PRIMER_PAIR_2_PENALTY=0.649166 PRIMER_LEFT_2_PENALTY=0.298018 PRIMER_RIGHT_2_PENALTY=0.351148 PRIMER_INTERNAL_2_PENALTY=0.229181 PRIMER_LEFT_2_SEQUENCE=ACCTGTCCACTTCTTGGCAG PRIMER_RIGHT_2_SEQUENCE=TCCTAAGGACACTCCCAGGA PRIMER_INTERNAL_2_SEQUENCE=TCTCAGTCACCAAGGGAAGG PRIMER_LEFT_2=69,20 PRIMER_RIGHT_2=154,20 PRIMER_INTERNAL_2=96,20 PRIMER_LEFT_2_TM=60.298 PRIMER_RIGHT_2_TM=59.649 PRIMER_INTERNAL_2_TM=60.229 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=5.00 PRIMER_PAIR_2_PRODUCT_SIZE=86 PRIMER_PAIR_3_PENALTY=0.669140 PRIMER_LEFT_3_PENALTY=0.229181 PRIMER_RIGHT_3_PENALTY=0.439959 PRIMER_INTERNAL_3_PENALTY=0.351148 PRIMER_LEFT_3_SEQUENCE=TCTCAGTCACCAAGGGAAGG PRIMER_RIGHT_3_SEQUENCE=TCAAGGTGAGCCAACAGTCA PRIMER_INTERNAL_3_SEQUENCE=TCCTGGGAGTGTCCTTAGGA PRIMER_LEFT_3=96,20 PRIMER_RIGHT_3=177,20 PRIMER_INTERNAL_3=135,20 PRIMER_LEFT_3_TM=60.229 PRIMER_RIGHT_3_TM=60.440 PRIMER_INTERNAL_3_TM=59.649 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=7.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=82 PRIMER_PAIR_4_PENALTY=0.681761 PRIMER_LEFT_4_PENALTY=0.158723 PRIMER_RIGHT_4_PENALTY=0.523038 PRIMER_INTERNAL_4_PENALTY=0.229181 PRIMER_LEFT_4_SEQUENCE=GTGCCAACCTGTCCACTTCT PRIMER_RIGHT_4_SEQUENCE=CCCAGGAAGAGTTGCTCTGA PRIMER_INTERNAL_4_SEQUENCE=TCTCAGTCACCAAGGGAAGG PRIMER_LEFT_4=63,20 PRIMER_RIGHT_4=141,20 PRIMER_INTERNAL_4=96,20 PRIMER_LEFT_4_TM=60.159 PRIMER_RIGHT_4_TM=60.523 PRIMER_INTERNAL_4_TM=60.229 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH974 SEQUENCE_TEMPLATE=CTGAAGGAGAGGAACCTCCAAATACAAACCTTGTTGGTTTTCGTGATTCTTGTGGCATTCAATGTTAAATGCTTCTGCCCCGAGGCTTGTGGCAATTGGTTAATATTGATGTCAGTGCCCTTCCCTCCTGTGTATTTTCGCTGGGAAAGTTTGAGAGATAAGGGGAGTTGATGTGTACAACTTAGTTTGAAAAGAACTTGTTCTCTCCTAGCCACTGAAAACCGCGTTGCTTTCTGCTTCCCATTCTTCCTTTACTGATAATCTGGCCATCACTCTGCTGTTTAAAAGTCTTGCTAGAGTATGGAGACAAG SEQUENCE_INCLUDED_REGION=0,311 P3_COMMENT=CL is GAAGGAGAGGAACCTCCAAA P3_COMMENT=CR is GTCTCCATACTCTAGCAAGACTTT P3_COMMENT=CM is ACAAACCTTGTTGGTTTTCG P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.297585 PRIMER_LEFT_0_PENALTY=0.131140 PRIMER_RIGHT_0_PENALTY=0.166445 PRIMER_INTERNAL_0_PENALTY=0.044832 PRIMER_LEFT_0_SEQUENCE=CTCCTAGCCACTGAAAACCG PRIMER_RIGHT_0_SEQUENCE=AGCAGAGTGATGGCCAGATT PRIMER_INTERNAL_0_SEQUENCE=TGCTTTCTGCTTCCCATTCT PRIMER_LEFT_0=204,20 PRIMER_RIGHT_0=278,20 PRIMER_INTERNAL_0=227,20 PRIMER_LEFT_0_TM=59.869 PRIMER_RIGHT_0_TM=59.834 PRIMER_INTERNAL_0_TM=59.955 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=0.300641 PRIMER_LEFT_1_PENALTY=0.234379 PRIMER_RIGHT_1_PENALTY=0.066262 PRIMER_INTERNAL_1_PENALTY=0.501869 PRIMER_LEFT_1_SEQUENCE=TTTTCGTGATTCTTGTGGCA PRIMER_RIGHT_1_SEQUENCE=GGAAGGGCACTGACATCAAT PRIMER_INTERNAL_1_SEQUENCE=AGGCTTGTGGCAATTGGTTA PRIMER_LEFT_1=37,20 PRIMER_RIGHT_1=123,20 PRIMER_INTERNAL_1=82,20 PRIMER_LEFT_1_TM=60.234 PRIMER_RIGHT_1_TM=59.934 PRIMER_INTERNAL_1_TM=60.502 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=10.0000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=87 PRIMER_PAIR_2_PENALTY=0.412026 PRIMER_LEFT_2_PENALTY=0.345764 PRIMER_RIGHT_2_PENALTY=0.066262 PRIMER_INTERNAL_2_PENALTY=0.501869 PRIMER_LEFT_2_SEQUENCE=TCGTGATTCTTGTGGCATTC PRIMER_RIGHT_2_SEQUENCE=GGAAGGGCACTGACATCAAT PRIMER_INTERNAL_2_SEQUENCE=AGGCTTGTGGCAATTGGTTA PRIMER_LEFT_2=40,20 PRIMER_RIGHT_2=123,20 PRIMER_INTERNAL_2=82,20 PRIMER_LEFT_2_TM=59.654 PRIMER_RIGHT_2_TM=59.934 PRIMER_INTERNAL_2_TM=60.502 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=84 PRIMER_PAIR_3_PENALTY=0.488010 PRIMER_LEFT_3_PENALTY=0.356870 PRIMER_RIGHT_3_PENALTY=0.131140 PRIMER_INTERNAL_3_PENALTY=3.964821 PRIMER_LEFT_3_SEQUENCE=TTTCGCTGGGAAAGTTTGAG PRIMER_RIGHT_3_SEQUENCE=CGGTTTTCAGTGGCTAGGAG PRIMER_INTERNAL_3_SEQUENCE=AAGGGGAGTTGATGTGTACAA PRIMER_LEFT_3=135,20 PRIMER_RIGHT_3=223,20 PRIMER_INTERNAL_3=159,21 PRIMER_LEFT_3_TM=60.357 PRIMER_RIGHT_3_TM=59.869 PRIMER_INTERNAL_3_TM=57.035 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=89 PRIMER_PAIR_4_PENALTY=0.561912 PRIMER_LEFT_4_PENALTY=0.234379 PRIMER_RIGHT_4_PENALTY=0.327533 PRIMER_INTERNAL_4_PENALTY=0.501869 PRIMER_LEFT_4_SEQUENCE=TTTTCGTGATTCTTGTGGCA PRIMER_RIGHT_4_SEQUENCE=AATACACAGGAGGGAAGGGC PRIMER_INTERNAL_4_SEQUENCE=AGGCTTGTGGCAATTGGTTA PRIMER_LEFT_4=37,20 PRIMER_RIGHT_4=135,20 PRIMER_INTERNAL_4=82,20 PRIMER_LEFT_4_TM=60.234 PRIMER_RIGHT_4_TM=60.328 PRIMER_INTERNAL_4_TM=60.502 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=10.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = SEQUENCE_ID=MH976 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCCAGCCCCTCTGTGTTGCTTTAGCCAAAGGTTAAGTTTACCTCCTCCAGGAGCCTTCTCTGATAACCTCAGCTGACACTGATATCCCCCATCAACTGACTGAAGGCAAGGATCTTGTCTTATACCACTTTGTAGTTTCCTAGAGTCTTACATTTTGAATTTAGTGATAACTGAAAAACTACCCCTTATTTCTTAAACTATTACACAGGTAATTACATAGAGTTTAAAAGTTTCTTAAATACATGTCATTTAAAAAGTAGTTGTTCATGAAGTGTTAAG SEQUENCE_INCLUDED_REGION=36,280 P3_COMMENT=CL is CAGCTGACACTGATATCCCC P3_COMMENT=CR is TCATGAACAACTACTTTTTAAATGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.450612 PRIMER_LEFT_0_PENALTY=0.124655 PRIMER_RIGHT_0_PENALTY=0.325957 PRIMER_INTERNAL_0_PENALTY=0.073866 PRIMER_LEFT_0_SEQUENCE=CCCTCTGTGTTGCTTTAGCC PRIMER_RIGHT_0_SEQUENCE=TCAGTCAGTTGATGGGGGAT PRIMER_INTERNAL_0_SEQUENCE=GTTTACCTCCTCCAGGAGCC PRIMER_LEFT_0=43,20 PRIMER_RIGHT_0=139,20 PRIMER_INTERNAL_0=72,20 PRIMER_LEFT_0_TM=59.875 PRIMER_RIGHT_0_TM=60.326 PRIMER_INTERNAL_0_TM=60.074 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=9.3000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=97 PRIMER_PAIR_1_PENALTY=0.450612 PRIMER_LEFT_1_PENALTY=0.124655 PRIMER_RIGHT_1_PENALTY=0.325957 PRIMER_INTERNAL_1_PENALTY=0.073866 PRIMER_LEFT_1_SEQUENCE=CCCCTCTGTGTTGCTTTAGC PRIMER_RIGHT_1_SEQUENCE=TCAGTCAGTTGATGGGGGAT PRIMER_INTERNAL_1_SEQUENCE=GTTTACCTCCTCCAGGAGCC PRIMER_LEFT_1=42,20 PRIMER_RIGHT_1=139,20 PRIMER_INTERNAL_1=72,20 PRIMER_LEFT_1_TM=59.875 PRIMER_RIGHT_1_TM=60.326 PRIMER_INTERNAL_1_TM=60.074 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.5000 PRIMER_RIGHT_1_END_STABILITY=9.3000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=98 PRIMER_PAIR_2_PENALTY=0.450612 PRIMER_LEFT_2_PENALTY=0.124655 PRIMER_RIGHT_2_PENALTY=0.325957 PRIMER_INTERNAL_2_PENALTY=0.073866 PRIMER_LEFT_2_SEQUENCE=GCCCCTCTGTGTTGCTTTAG PRIMER_RIGHT_2_SEQUENCE=TCAGTCAGTTGATGGGGGAT PRIMER_INTERNAL_2_SEQUENCE=GTTTACCTCCTCCAGGAGCC PRIMER_LEFT_2=41,20 PRIMER_RIGHT_2=139,20 PRIMER_INTERNAL_2=72,20 PRIMER_LEFT_2_TM=59.875 PRIMER_RIGHT_2_TM=60.326 PRIMER_INTERNAL_2_TM=60.074 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.3000 PRIMER_RIGHT_2_END_STABILITY=9.3000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=99 PRIMER_PAIR_3_PENALTY=0.450612 PRIMER_LEFT_3_PENALTY=0.124655 PRIMER_RIGHT_3_PENALTY=0.325957 PRIMER_INTERNAL_3_PENALTY=0.073866 PRIMER_LEFT_3_SEQUENCE=AGCCCCTCTGTGTTGCTTTA PRIMER_RIGHT_3_SEQUENCE=TCAGTCAGTTGATGGGGGAT PRIMER_INTERNAL_3_SEQUENCE=GTTTACCTCCTCCAGGAGCC PRIMER_LEFT_3=40,20 PRIMER_RIGHT_3=139,20 PRIMER_INTERNAL_3=72,20 PRIMER_LEFT_3_TM=59.875 PRIMER_RIGHT_3_TM=60.326 PRIMER_INTERNAL_3_TM=60.074 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.3000 PRIMER_RIGHT_3_END_STABILITY=9.3000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=100 PRIMER_PAIR_4_PENALTY=0.625483 PRIMER_LEFT_4_PENALTY=0.124655 PRIMER_RIGHT_4_PENALTY=0.500828 PRIMER_INTERNAL_4_PENALTY=0.073866 PRIMER_LEFT_4_SEQUENCE=CCCTCTGTGTTGCTTTAGCC PRIMER_RIGHT_4_SEQUENCE=CTTCAGTCAGTTGATGGGGG PRIMER_INTERNAL_4_SEQUENCE=GTTTACCTCCTCCAGGAGCC PRIMER_LEFT_4=43,20 PRIMER_RIGHT_4=141,20 PRIMER_INTERNAL_4=72,20 PRIMER_LEFT_4_TM=59.875 PRIMER_RIGHT_4_TM=60.501 PRIMER_INTERNAL_4_TM=60.074 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.7000 PRIMER_RIGHT_4_END_STABILITY=12.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = SEQUENCE_ID=MH977 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTGATAAGTACAGTTCAGCCTTTTCTCCTCAAATATATAATGACTTTAACATTCCTAAGAATATAGGTATTTCTGAATGATTTAAATTTGAGGAATTTTAATACATAAAATACAATGTACAAACTTTCTGCCCACTCAGATCTCTTCTCCATCATGTACTTAGTATTTCCCATTAACCTACACACTGATTTTTATGCTACTCCTTGTAGAAACAAAATTCTGGTTTGACTCAGTTTTTGTGTTTATAAACTTTTGGAATGTGTACCCCGTTTATGTGAAGAATTATGACCTATCAGTCATAG SEQUENCE_INCLUDED_REGION=36,302 P3_COMMENT=CL is CTGATAAGTACAGTTCAGCCTTT P3_COMMENT=CR is ATGACTGATAGGTCATAATTCTTCA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.481595 PRIMER_LEFT_0_PENALTY=2.032347 PRIMER_RIGHT_0_PENALTY=4.449248 PRIMER_INTERNAL_0_PENALTY=5.526775 PRIMER_LEFT_0_SEQUENCE=CAATGTACAAACTTTCTGCCCA PRIMER_RIGHT_0_SEQUENCE=TCAGTGTGTAGGTTAATGGGAAA PRIMER_INTERNAL_0_SEQUENCE=CTCAGATCTCTTCTCCATCATGT PRIMER_LEFT_0=148,22 PRIMER_RIGHT_0=223,23 PRIMER_INTERNAL_0=170,23 PRIMER_LEFT_0_TM=60.032 PRIMER_RIGHT_0_TM=58.551 PRIMER_INTERNAL_0_TM=57.473 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=39.130 PRIMER_INTERNAL_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=11.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 PRIMER_PAIR_1_PENALTY=6.809408 PRIMER_LEFT_1_PENALTY=6.720996 PRIMER_RIGHT_1_PENALTY=0.088413 PRIMER_INTERNAL_1_PENALTY=3.685690 PRIMER_LEFT_1_SEQUENCE=CACTGATTTTTATGCTACTCCTTG PRIMER_RIGHT_1_SEQUENCE=ACGGGGTACACATTCCAAAA PRIMER_INTERNAL_1_SEQUENCE=TCTGGTTTGACTCAGTTTTTGTG PRIMER_LEFT_1=218,24 PRIMER_RIGHT_1=305,20 PRIMER_INTERNAL_1=254,23 PRIMER_LEFT_1_TM=57.279 PRIMER_RIGHT_1_TM=60.088 PRIMER_INTERNAL_1_TM=59.314 PRIMER_LEFT_1_GC_PERCENT=37.500 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=88 PRIMER_PAIR_2_PENALTY=6.809408 PRIMER_LEFT_2_PENALTY=6.720996 PRIMER_RIGHT_2_PENALTY=0.088413 PRIMER_INTERNAL_2_PENALTY=3.685690 PRIMER_LEFT_2_SEQUENCE=CACTGATTTTTATGCTACTCCTTG PRIMER_RIGHT_2_SEQUENCE=AACGGGGTACACATTCCAAA PRIMER_INTERNAL_2_SEQUENCE=TCTGGTTTGACTCAGTTTTTGTG PRIMER_LEFT_2=218,24 PRIMER_RIGHT_2=306,20 PRIMER_INTERNAL_2=254,23 PRIMER_LEFT_2_TM=57.279 PRIMER_RIGHT_2_TM=60.088 PRIMER_INTERNAL_2_TM=59.314 PRIMER_LEFT_2_GC_PERCENT=37.500 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=8.8000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=89 PRIMER_PAIR_3_PENALTY=6.809408 PRIMER_LEFT_3_PENALTY=6.720996 PRIMER_RIGHT_3_PENALTY=0.088413 PRIMER_INTERNAL_3_PENALTY=3.685690 PRIMER_LEFT_3_SEQUENCE=CACTGATTTTTATGCTACTCCTTG PRIMER_RIGHT_3_SEQUENCE=AAACGGGGTACACATTCCAA PRIMER_INTERNAL_3_SEQUENCE=TCTGGTTTGACTCAGTTTTTGTG PRIMER_LEFT_3=218,24 PRIMER_RIGHT_3=307,20 PRIMER_INTERNAL_3=254,23 PRIMER_LEFT_3_TM=57.279 PRIMER_RIGHT_3_TM=60.088 PRIMER_INTERNAL_3_TM=59.314 PRIMER_LEFT_3_GC_PERCENT=37.500 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=90 PRIMER_PAIR_4_PENALTY=6.842323 PRIMER_LEFT_4_PENALTY=1.351236 PRIMER_RIGHT_4_PENALTY=5.491087 PRIMER_INTERNAL_4_PENALTY=4.449248 PRIMER_LEFT_4_SEQUENCE=GCCCACTCAGATCTCTTCTCC PRIMER_RIGHT_4_SEQUENCE=CCAGAATTTTGTTTCTACAAGGA PRIMER_INTERNAL_4_SEQUENCE=TTTCCCATTAACCTACACACTGA PRIMER_LEFT_4=165,21 PRIMER_RIGHT_4=258,23 PRIMER_INTERNAL_4=201,23 PRIMER_LEFT_4_TM=60.351 PRIMER_RIGHT_4_TM=57.509 PRIMER_INTERNAL_4_TM=58.551 PRIMER_LEFT_4_GC_PERCENT=57.143 PRIMER_RIGHT_4_GC_PERCENT=34.783 PRIMER_INTERNAL_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=94 = SEQUENCE_ID=MH978 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTAGAGAGAAGTCAAAGCCTGGCTTCAAAGCTTCAAAGGACAGGCTCATTCCCTTGTTAGGGGCTAATGCAGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTG SEQUENCE_INCLUDED_REGION=36,71 P3_COMMENT=CL is P3_COMMENT=CR is P3_COMMENT=CM is _ P3_COMMENT=CC is 0,_ PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=MH979 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTGGTACTTGTGATGAATGTATTTGTTCTCATTGGTTCCAGAGCATTTAAAAGTGTAAGTTGATATCAGGATTTCCTTCACCTTTTGGTCACATACTTTGCCAACTGTTAGACATTTGCGTATTTTAAATCCTACAGTTTTAGCGTGTCTTGGGCCTGTCATCTTTGCTAGTACACGTTGCTGTACTTGTCTGTTAATCCCTAGATCTTCAAAGTACAAAGAATGGTTCTAATAGGTATTGTTATCACATATAAGAAAGAACATCACATAGAAGTTTAGAGTTGTAAGGACTCTAAGGATGATCTAGCCCAAACCC SEQUENCE_INCLUDED_REGION=36,315 P3_COMMENT=CL is GATGAATGTATTTGTTCTCATTGG P3_COMMENT=CR is GGTTTGGGCTAGATCATCCT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.011171 PRIMER_LEFT_0_PENALTY=0.891362 PRIMER_RIGHT_0_PENALTY=0.119809 PRIMER_INTERNAL_0_PENALTY=1.683262 PRIMER_LEFT_0_SEQUENCE=TCCTTCACCTTTTGGTCACA PRIMER_RIGHT_0_SEQUENCE=AGATGACAGGCCCAAGACAC PRIMER_INTERNAL_0_SEQUENCE=GCCAACTGTTAGACATTTGCG PRIMER_LEFT_0=108,20 PRIMER_RIGHT_0=198,20 PRIMER_INTERNAL_0=134,21 PRIMER_LEFT_0_TM=59.109 PRIMER_RIGHT_0_TM=60.120 PRIMER_INTERNAL_0_TM=60.683 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=91 PRIMER_PAIR_1_PENALTY=1.055020 PRIMER_LEFT_1_PENALTY=0.891362 PRIMER_RIGHT_1_PENALTY=0.163658 PRIMER_INTERNAL_1_PENALTY=1.683262 PRIMER_LEFT_1_SEQUENCE=TCCTTCACCTTTTGGTCACA PRIMER_RIGHT_1_SEQUENCE=CTAGCAAAGATGACAGGCCC PRIMER_INTERNAL_1_SEQUENCE=GCCAACTGTTAGACATTTGCG PRIMER_LEFT_1=108,20 PRIMER_RIGHT_1=205,20 PRIMER_INTERNAL_1=134,21 PRIMER_LEFT_1_TM=59.109 PRIMER_RIGHT_1_TM=59.836 PRIMER_INTERNAL_1_TM=60.683 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=12.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=98 PRIMER_PAIR_2_PENALTY=1.508756 PRIMER_LEFT_2_PENALTY=0.891362 PRIMER_RIGHT_2_PENALTY=0.617395 PRIMER_INTERNAL_2_PENALTY=1.683262 PRIMER_LEFT_2_SEQUENCE=TCCTTCACCTTTTGGTCACA PRIMER_RIGHT_2_SEQUENCE=GCCCAAGACACGCTAAAACT PRIMER_INTERNAL_2_SEQUENCE=GCCAACTGTTAGACATTTGCG PRIMER_LEFT_2=108,20 PRIMER_RIGHT_2=189,20 PRIMER_INTERNAL_2=134,21 PRIMER_LEFT_2_TM=59.109 PRIMER_RIGHT_2_TM=59.383 PRIMER_INTERNAL_2_TM=60.683 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=82 PRIMER_PAIR_3_PENALTY=1.547754 PRIMER_LEFT_3_PENALTY=0.891362 PRIMER_RIGHT_3_PENALTY=0.656393 PRIMER_INTERNAL_3_PENALTY=1.683262 PRIMER_LEFT_3_SEQUENCE=TCCTTCACCTTTTGGTCACA PRIMER_RIGHT_3_SEQUENCE=AAGATGACAGGCCCAAGACA PRIMER_INTERNAL_3_SEQUENCE=GCCAACTGTTAGACATTTGCG PRIMER_LEFT_3=108,20 PRIMER_RIGHT_3=199,20 PRIMER_INTERNAL_3=134,21 PRIMER_LEFT_3_TM=59.109 PRIMER_RIGHT_3_TM=60.656 PRIMER_INTERNAL_3_TM=60.683 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=92 PRIMER_PAIR_4_PENALTY=1.633444 PRIMER_LEFT_4_PENALTY=1.513635 PRIMER_RIGHT_4_PENALTY=0.119809 PRIMER_INTERNAL_4_PENALTY=1.683262 PRIMER_LEFT_4_SEQUENCE=TTCCTTCACCTTTTGGTCACA PRIMER_RIGHT_4_SEQUENCE=AGATGACAGGCCCAAGACAC PRIMER_INTERNAL_4_SEQUENCE=GCCAACTGTTAGACATTTGCG PRIMER_LEFT_4=107,21 PRIMER_RIGHT_4=198,20 PRIMER_INTERNAL_4=134,21 PRIMER_LEFT_4_TM=60.514 PRIMER_RIGHT_4_TM=60.120 PRIMER_INTERNAL_4_TM=60.683 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.1000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=92 = SEQUENCE_ID=MH982 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTGACCTTTTAAGTGAACTATCTGGCACTGTTTGAAATTGCATCAGCCAGTTGGAATAAGAATTCGCTCTCCTGTGAGACTATTCTTATACAGCATCATGTTATGTGTGTAATAAAGTCATATTTGGCCTTTGCCCCCATGTCACATCACAGAGCCCCATTCCTGATAAGAGTGTCCTTTGTTATTCATAGCGAGCCCCTGTTAACCATACCTAAGTTTGTTCTAATGTGGTAACTCACTGTAGGCACCTGGGTAG SEQUENCE_INCLUDED_REGION=36,257 P3_COMMENT=CL is ACTGTTTGAAATTGCATCAGC P3_COMMENT=CR is CCAGGTGCCTACAGTGAGTT P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.233598 PRIMER_LEFT_0_PENALTY=0.059762 PRIMER_RIGHT_0_PENALTY=0.173836 PRIMER_INTERNAL_0_PENALTY=6.660950 PRIMER_LEFT_0_SEQUENCE=TGTTATTCATAGCGAGCCCC PRIMER_RIGHT_0_SEQUENCE=ACCCAGGTGCCTACAGTGAG PRIMER_INTERNAL_0_SEQUENCE=CCATACCTAAGTTTGTTCTAATGTGG PRIMER_LEFT_0=216,20 PRIMER_RIGHT_0=290,20 PRIMER_INTERNAL_0=242,26 PRIMER_LEFT_0_TM=60.060 PRIMER_RIGHT_0_TM=60.174 PRIMER_INTERNAL_0_TM=59.339 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_GC_PERCENT=38.462 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=0.593264 PRIMER_LEFT_1_PENALTY=0.045756 PRIMER_RIGHT_1_PENALTY=0.547508 PRIMER_INTERNAL_1_PENALTY=2.173354 PRIMER_LEFT_1_SEQUENCE=CCCCATGTCACATCACAGAG PRIMER_RIGHT_1_SEQUENCE=GTATGGTTAACAGGGGCTCG PRIMER_INTERNAL_1_SEQUENCE=CCCATTCCTGATAAGAGTGTCC PRIMER_LEFT_1=171,20 PRIMER_RIGHT_1=247,20 PRIMER_INTERNAL_1=192,22 PRIMER_LEFT_1_TM=59.954 PRIMER_RIGHT_1_TM=59.452 PRIMER_INTERNAL_1_TM=59.827 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=9.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=0.668902 PRIMER_LEFT_2_PENALTY=0.121394 PRIMER_RIGHT_2_PENALTY=0.547508 PRIMER_INTERNAL_2_PENALTY=4.099075 PRIMER_LEFT_2_SEQUENCE=CCATGTCACATCACAGAGCC PRIMER_RIGHT_2_SEQUENCE=GTATGGTTAACAGGGGCTCG PRIMER_INTERNAL_2_SEQUENCE=CCATTCCTGATAAGAGTGTCCTTT PRIMER_LEFT_2=173,20 PRIMER_RIGHT_2=247,20 PRIMER_INTERNAL_2=193,24 PRIMER_LEFT_2_TM=60.121 PRIMER_RIGHT_2_TM=59.452 PRIMER_INTERNAL_2_TM=59.901 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=0.668902 PRIMER_LEFT_3_PENALTY=0.121394 PRIMER_RIGHT_3_PENALTY=0.547508 PRIMER_INTERNAL_3_PENALTY=2.173354 PRIMER_LEFT_3_SEQUENCE=CCCATGTCACATCACAGAGC PRIMER_RIGHT_3_SEQUENCE=GTATGGTTAACAGGGGCTCG PRIMER_INTERNAL_3_SEQUENCE=CCCATTCCTGATAAGAGTGTCC PRIMER_LEFT_3=172,20 PRIMER_RIGHT_3=247,20 PRIMER_INTERNAL_3=192,22 PRIMER_LEFT_3_TM=60.121 PRIMER_RIGHT_3_TM=59.452 PRIMER_INTERNAL_3_TM=59.827 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=9.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=76 PRIMER_PAIR_4_PENALTY=0.898714 PRIMER_LEFT_4_PENALTY=0.059762 PRIMER_RIGHT_4_PENALTY=0.838952 PRIMER_INTERNAL_4_PENALTY=6.660950 PRIMER_LEFT_4_SEQUENCE=TGTTATTCATAGCGAGCCCC PRIMER_RIGHT_4_SEQUENCE=TACCCAGGTGCCTACAGTGA PRIMER_INTERNAL_4_SEQUENCE=CCATACCTAAGTTTGTTCTAATGTGG PRIMER_LEFT_4=216,20 PRIMER_RIGHT_4=291,20 PRIMER_INTERNAL_4=242,26 PRIMER_LEFT_4_TM=60.060 PRIMER_RIGHT_4_TM=59.161 PRIMER_INTERNAL_4_TM=59.339 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=38.462 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=12.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=MH986 SEQUENCE_TEMPLATE=CTTCATGCTCCAGGTCGACTCTAGAGGATCCCTGCTGTCTGGGCCCGGCAAATTGGTTTAAGTGGAATGAAATTGGTTCCTTCCCTCCCCGTGCTCATGGCTGAGGTCCAGGCCCGATTGTAATGGGAACCTCTCCTGGGCAAAGGACATAGAAAAGCTTTCCGGGGAGTCTGACCCTCTTTTTTCATATGTGAATTCTACTTNCCCCTGGGGGTCAAGGCAATAGGCTGATAAAACATANAAATGAACCTGAAGTGGGTGGGGTGAGTGTGTGTGTGGGAAGTTGGATCAGAGGAGAATGAACAATGAACAGTGGCCGAGTGTTTTTCAAGGGCCAGTTAACCATTCCCCTGTTG SEQUENCE_INCLUDED_REGION=32,324 P3_COMMENT=CL is GGCAAATTGGTTTAAGTGGA P3_COMMENT=CR is ACAGGGGAATGGTTAACTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.289767 PRIMER_LEFT_0_PENALTY=0.255861 PRIMER_RIGHT_0_PENALTY=0.033905 PRIMER_INTERNAL_0_PENALTY=0.755541 PRIMER_LEFT_0_SEQUENCE=GAAATTGGTTCCTTCCCTCC PRIMER_RIGHT_0_SEQUENCE=TCCCCGGAAAGCTTTTCTAT PRIMER_INTERNAL_0_SEQUENCE=CCGATTGTAATGGGAACCTC PRIMER_LEFT_0=68,20 PRIMER_RIGHT_0=167,20 PRIMER_INTERNAL_0=113,20 PRIMER_LEFT_0_TM=59.744 PRIMER_RIGHT_0_TM=60.034 PRIMER_INTERNAL_0_TM=59.244 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=5.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=0.297318 PRIMER_LEFT_1_PENALTY=0.110369 PRIMER_RIGHT_1_PENALTY=0.186949 PRIMER_INTERNAL_1_PENALTY=0.156287 PRIMER_LEFT_1_SEQUENCE=GTGTGTGTGTGGGAAGTTGG PRIMER_RIGHT_1_SEQUENCE=GGAATGGTTAACTGGCCCTT PRIMER_INTERNAL_1_SEQUENCE=ACAGTGGCCGAGTGTTTTTC PRIMER_LEFT_1=267,20 PRIMER_RIGHT_1=348,20 PRIMER_INTERNAL_1=309,20 PRIMER_LEFT_1_TM=59.890 PRIMER_RIGHT_1_TM=60.187 PRIMER_INTERNAL_1_TM=60.156 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 PRIMER_PAIR_2_PENALTY=0.322184 PRIMER_LEFT_2_PENALTY=0.255861 PRIMER_RIGHT_2_PENALTY=0.066323 PRIMER_INTERNAL_2_PENALTY=0.755541 PRIMER_LEFT_2_SEQUENCE=GAAATTGGTTCCTTCCCTCC PRIMER_RIGHT_2_SEQUENCE=TTCTATGTCCTTTGCCCAGG PRIMER_INTERNAL_2_SEQUENCE=CCGATTGTAATGGGAACCTC PRIMER_LEFT_2=68,20 PRIMER_RIGHT_2=153,20 PRIMER_INTERNAL_2=113,20 PRIMER_LEFT_2_TM=59.744 PRIMER_RIGHT_2_TM=60.066 PRIMER_INTERNAL_2_TM=59.244 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=86 PRIMER_PAIR_3_PENALTY=0.335962 PRIMER_LEFT_3_PENALTY=0.179674 PRIMER_RIGHT_3_PENALTY=0.156287 PRIMER_INTERNAL_3_PENALTY=0.110369 PRIMER_LEFT_3_SEQUENCE=AATGAACCTGAAGTGGGTGG PRIMER_RIGHT_3_SEQUENCE=AAAACACTCGGCCACTGTTC PRIMER_INTERNAL_3_SEQUENCE=GTGTGTGTGTGGGAAGTTGG PRIMER_LEFT_3=242,20 PRIMER_RIGHT_3=326,20 PRIMER_INTERNAL_3=267,20 PRIMER_LEFT_3_TM=59.820 PRIMER_RIGHT_3_TM=60.156 PRIMER_INTERNAL_3_TM=59.890 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=85 PRIMER_PAIR_4_PENALTY=0.335962 PRIMER_LEFT_4_PENALTY=0.179674 PRIMER_RIGHT_4_PENALTY=0.156287 PRIMER_INTERNAL_4_PENALTY=0.110369 PRIMER_LEFT_4_SEQUENCE=AATGAACCTGAAGTGGGTGG PRIMER_RIGHT_4_SEQUENCE=GAAAAACACTCGGCCACTGT PRIMER_INTERNAL_4_SEQUENCE=GTGTGTGTGTGGGAAGTTGG PRIMER_LEFT_4=242,20 PRIMER_RIGHT_4=328,20 PRIMER_INTERNAL_4=267,20 PRIMER_LEFT_4_TM=59.820 PRIMER_RIGHT_4_TM=60.156 PRIMER_INTERNAL_4_TM=59.890 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=6.1000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH989 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTTATAATTTTCCCACTGAGTATGGCTTTCAGTAGTTTTATTATGATGTGCCTAGGTACATTTGTTTTTATTTGTTCTGCGAATTGTTGTATTACTTTGGGAGAAATGCTCAACTATAAATATTGCTTCTGACCCTTTTCTGTGTTCCTTCTTAAAGATACAAAATAAATGTAACATTAGACCTCTCACTATGCTGTTTTTACTCTCCTCTGATTTTTTTTTCCATTATTTTTATTGCTCTGGCTTCATTTTGTAAATNTGCTTCTTGAAATTTTTCTTTTAATTCTAGAATATCTATTTGGTTCTTTTCATGGTTTCTAGTTCTCTGAAAATG SEQUENCE_INCLUDED_REGION=33,336 P3_COMMENT=CL is TCCCACTGAGTATGGCTTTC P3_COMMENT=CR is CTAGAAACCATGAAAAGAACCA P3_COMMENT=CM is TTTTATTATGATGTGCCTAGGTACA P3_COMMENT=CC is 1,1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.462135 PRIMER_LEFT_0_PENALTY=1.257221 PRIMER_RIGHT_0_PENALTY=3.204914 PRIMER_INTERNAL_0_PENALTY=4.166289 PRIMER_LEFT_0_SEQUENCE=CCCACTGAGTATGGCTTTCAG PRIMER_RIGHT_0_SEQUENCE=CCCAAAGTAATACAACAATTCGC PRIMER_INTERNAL_0_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT PRIMER_LEFT_0=46,21 PRIMER_RIGHT_0=135,23 PRIMER_INTERNAL_0=78,24 PRIMER_LEFT_0_TM=59.743 PRIMER_RIGHT_0_TM=59.795 PRIMER_INTERNAL_0_TM=59.834 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=39.130 PRIMER_INTERNAL_0_GC_PERCENT=37.500 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=10.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=90 PRIMER_PAIR_1_PENALTY=4.483080 PRIMER_LEFT_1_PENALTY=1.278166 PRIMER_RIGHT_1_PENALTY=3.204914 PRIMER_INTERNAL_1_PENALTY=4.166289 PRIMER_LEFT_1_SEQUENCE=CCCACTGAGTATGGCTTTCA PRIMER_RIGHT_1_SEQUENCE=CCCAAAGTAATACAACAATTCGC PRIMER_INTERNAL_1_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT PRIMER_LEFT_1=46,20 PRIMER_RIGHT_1=135,23 PRIMER_INTERNAL_1=78,24 PRIMER_LEFT_1_TM=58.722 PRIMER_RIGHT_1_TM=59.795 PRIMER_INTERNAL_1_TM=59.834 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=39.130 PRIMER_INTERNAL_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=12.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=10.2000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=90 PRIMER_PAIR_2_PENALTY=4.846658 PRIMER_LEFT_2_PENALTY=1.641744 PRIMER_RIGHT_2_PENALTY=3.204914 PRIMER_INTERNAL_2_PENALTY=4.166289 PRIMER_LEFT_2_SEQUENCE=TCCCACTGAGTATGGCTTTCA PRIMER_RIGHT_2_SEQUENCE=CCCAAAGTAATACAACAATTCGC PRIMER_INTERNAL_2_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT PRIMER_LEFT_2=45,21 PRIMER_RIGHT_2=135,23 PRIMER_INTERNAL_2=78,24 PRIMER_LEFT_2_TM=60.642 PRIMER_RIGHT_2_TM=59.795 PRIMER_INTERNAL_2_TM=59.834 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=39.130 PRIMER_INTERNAL_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=12.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=10.2000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=91 PRIMER_PAIR_3_PENALTY=4.969665 PRIMER_LEFT_3_PENALTY=1.257221 PRIMER_RIGHT_3_PENALTY=3.712444 PRIMER_INTERNAL_3_PENALTY=4.166289 PRIMER_LEFT_3_SEQUENCE=CCCACTGAGTATGGCTTTCAG PRIMER_RIGHT_3_SEQUENCE=ACAACAATTCGCAGAACAAAT PRIMER_INTERNAL_3_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT PRIMER_LEFT_3=46,21 PRIMER_RIGHT_3=124,21 PRIMER_INTERNAL_3=78,24 PRIMER_LEFT_3_TM=59.743 PRIMER_RIGHT_3_TM=57.288 PRIMER_INTERNAL_3_TM=59.834 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_INTERNAL_3_GC_PERCENT=37.500 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=12.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=4.990610 PRIMER_LEFT_4_PENALTY=1.278166 PRIMER_RIGHT_4_PENALTY=3.712444 PRIMER_INTERNAL_4_PENALTY=4.166289 PRIMER_LEFT_4_SEQUENCE=CCCACTGAGTATGGCTTTCA PRIMER_RIGHT_4_SEQUENCE=ACAACAATTCGCAGAACAAAT PRIMER_INTERNAL_4_SEQUENCE=TGATGTGCCTAGGTACATTTGTTT PRIMER_LEFT_4=46,20 PRIMER_RIGHT_4=124,21 PRIMER_INTERNAL_4=78,24 PRIMER_LEFT_4_TM=58.722 PRIMER_RIGHT_4_TM=57.288 PRIMER_INTERNAL_4_TM=59.834 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=33.333 PRIMER_INTERNAL_4_GC_PERCENT=37.500 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=12.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=7.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=79 = SEQUENCE_ID=MH991 SEQUENCE_TEMPLATE=CTTCTGCAGGACATTTGTACCATCGAGGTCAAAGGCAACAAGAAGTGACATGAGTTTCAGTCCATCTTCATGAGGTTCCAGTTAAGGCCTGTGAATTCGCAATTGTTCTTCCCGACTGCCTGTCCTGTGAACCTCACATACTCACATCAGATGCAAAAACAACCACCTTGTAGAAG SEQUENCE_INCLUDED_REGION=0,176 P3_COMMENT=CL is GAGGTCAAAGGCAACAAGAA P3_COMMENT=CR is AAGGTGGTTGTTTTTGCATC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.731126 PRIMER_LEFT_0_PENALTY=0.444466 PRIMER_RIGHT_0_PENALTY=0.286660 PRIMER_INTERNAL_0_PENALTY=0.991035 PRIMER_LEFT_0_SEQUENCE=GTACCATCGAGGTCAAAGGC PRIMER_RIGHT_0_SEQUENCE=GCGAATTCACAGGCCTTAAC PRIMER_INTERNAL_0_SEQUENCE=TCCATCTTCATGAGGTTCCA PRIMER_LEFT_0=16,20 PRIMER_RIGHT_0=99,20 PRIMER_INTERNAL_0=60,20 PRIMER_LEFT_0_TM=59.556 PRIMER_RIGHT_0_TM=59.713 PRIMER_INTERNAL_0_TM=59.009 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=84 PRIMER_PAIR_1_PENALTY=0.852407 PRIMER_LEFT_1_PENALTY=0.444466 PRIMER_RIGHT_1_PENALTY=0.407941 PRIMER_INTERNAL_1_PENALTY=3.274264 PRIMER_LEFT_1_SEQUENCE=GTACCATCGAGGTCAAAGGC PRIMER_RIGHT_1_SEQUENCE=CACAGGCCTTAACTGGAACC PRIMER_INTERNAL_1_SEQUENCE=TGAGTTTCAGTCCATCTTCATGA PRIMER_LEFT_1=16,20 PRIMER_RIGHT_1=92,20 PRIMER_INTERNAL_1=50,23 PRIMER_LEFT_1_TM=59.556 PRIMER_RIGHT_1_TM=59.592 PRIMER_INTERNAL_1_TM=59.726 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=77 PRIMER_PAIR_2_PENALTY=0.922693 PRIMER_LEFT_2_PENALTY=0.636033 PRIMER_RIGHT_2_PENALTY=0.286660 PRIMER_INTERNAL_2_PENALTY=0.991035 PRIMER_LEFT_2_SEQUENCE=ATCGAGGTCAAAGGCAACAA PRIMER_RIGHT_2_SEQUENCE=GCGAATTCACAGGCCTTAAC PRIMER_INTERNAL_2_SEQUENCE=TCCATCTTCATGAGGTTCCA PRIMER_LEFT_2=21,20 PRIMER_RIGHT_2=99,20 PRIMER_INTERNAL_2=60,20 PRIMER_LEFT_2_TM=60.636 PRIMER_RIGHT_2_TM=59.713 PRIMER_INTERNAL_2_TM=59.009 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=6.0000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=79 PRIMER_PAIR_3_PENALTY=1.214573 PRIMER_LEFT_3_PENALTY=0.286660 PRIMER_RIGHT_3_PENALTY=0.927913 PRIMER_INTERNAL_3_PENALTY=0.243525 PRIMER_LEFT_3_SEQUENCE=GTTAAGGCCTGTGAATTCGC PRIMER_RIGHT_3_SEQUENCE=ACAAGGTGGTTGTTTTTGCA PRIMER_INTERNAL_3_SEQUENCE=ACTGCCTGTCCTGTGAACCT PRIMER_LEFT_3=80,20 PRIMER_RIGHT_3=170,20 PRIMER_INTERNAL_3=114,20 PRIMER_LEFT_3_TM=59.713 PRIMER_RIGHT_3_TM=59.072 PRIMER_INTERNAL_3_TM=59.756 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=10.2000 PRIMER_RIGHT_3_END_STABILITY=8.8000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=91 PRIMER_PAIR_4_PENALTY=1.262277 PRIMER_LEFT_4_PENALTY=0.286660 PRIMER_RIGHT_4_PENALTY=0.975617 PRIMER_INTERNAL_4_PENALTY=0.243525 PRIMER_LEFT_4_SEQUENCE=GTTAAGGCCTGTGAATTCGC PRIMER_RIGHT_4_SEQUENCE=GGTGGTTGTTTTTGCATCTG PRIMER_INTERNAL_4_SEQUENCE=ACTGCCTGTCCTGTGAACCT PRIMER_LEFT_4=80,20 PRIMER_RIGHT_4=166,20 PRIMER_INTERNAL_4=114,20 PRIMER_LEFT_4_TM=59.713 PRIMER_RIGHT_4_TM=59.024 PRIMER_INTERNAL_4_TM=59.756 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=10.2000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=2.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=87 = SEQUENCE_ID=MH993 SEQUENCE_TEMPLATE=TGCATGCCTGCAGGTCGACTCTAGAGGATCCCCTAACCTTTTGTTTCTTTCTTTTTCAGTACACTGCTTTAATAAATGGTAACAGTGCTTTAATAAGTCCCCTGGCCATCATCTTACTTATGATCTCTAGATGAAGACCACTGTGTGAAACTGGTATCCTAGGAGGTTGGAGTGAGCATGTATGAGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGCTTGATTTATAAACCACTGAAGCCCGATGAAGTACTAGGGTGAAAATCACCTACGTTTCCAGAGGAAAAAAATGTCACCATCAATTCCAAGAGGGACTTGTAAACCTGAAACTAACAGCCAATGGTTTAATTTTTTTAAGGTAGAT SEQUENCE_INCLUDED_REGION=33,342 P3_COMMENT=CL is TTTCTTTCTTTTTCAGTACACTGC P3_COMMENT=CR is ACCTTAAAAAAATTAAACCATTGG P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.666116 PRIMER_LEFT_0_PENALTY=0.546307 PRIMER_RIGHT_0_PENALTY=0.119809 PRIMER_INTERNAL_0_PENALTY=2.335679 PRIMER_LEFT_0_SEQUENCE=ATAAGTCCCCTGGCCATCAT PRIMER_RIGHT_0_SEQUENCE=ACATGCTCACTCCAACCTCC PRIMER_INTERNAL_0_SEQUENCE=GAAGACCACTGTGTGAAACTGG PRIMER_LEFT_0=92,20 PRIMER_RIGHT_0=180,20 PRIMER_INTERNAL_0=132,22 PRIMER_LEFT_0_TM=60.546 PRIMER_RIGHT_0_TM=60.120 PRIMER_INTERNAL_0_TM=59.664 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.5000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=89 PRIMER_PAIR_1_PENALTY=0.713625 PRIMER_LEFT_1_PENALTY=0.593816 PRIMER_RIGHT_1_PENALTY=0.119809 PRIMER_INTERNAL_1_PENALTY=2.335679 PRIMER_LEFT_1_SEQUENCE=TTTAATAAGTCCCCTGGCCA PRIMER_RIGHT_1_SEQUENCE=ACATGCTCACTCCAACCTCC PRIMER_INTERNAL_1_SEQUENCE=GAAGACCACTGTGTGAAACTGG PRIMER_LEFT_1=88,20 PRIMER_RIGHT_1=180,20 PRIMER_INTERNAL_1=132,22 PRIMER_LEFT_1_TM=59.406 PRIMER_RIGHT_1_TM=60.120 PRIMER_INTERNAL_1_TM=59.664 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=11.2000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=93 PRIMER_PAIR_2_PENALTY=0.807063 PRIMER_LEFT_2_PENALTY=0.546307 PRIMER_RIGHT_2_PENALTY=0.260757 PRIMER_INTERNAL_2_PENALTY=2.335679 PRIMER_LEFT_2_SEQUENCE=ATAAGTCCCCTGGCCATCAT PRIMER_RIGHT_2_SEQUENCE=CATGCTCACTCCAACCTCCT PRIMER_INTERNAL_2_SEQUENCE=GAAGACCACTGTGTGAAACTGG PRIMER_LEFT_2=92,20 PRIMER_RIGHT_2=179,20 PRIMER_INTERNAL_2=132,22 PRIMER_LEFT_2_TM=60.546 PRIMER_RIGHT_2_TM=60.261 PRIMER_INTERNAL_2_TM=59.664 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.5000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=88 PRIMER_PAIR_3_PENALTY=0.854573 PRIMER_LEFT_3_PENALTY=0.593816 PRIMER_RIGHT_3_PENALTY=0.260757 PRIMER_INTERNAL_3_PENALTY=2.335679 PRIMER_LEFT_3_SEQUENCE=TTTAATAAGTCCCCTGGCCA PRIMER_RIGHT_3_SEQUENCE=CATGCTCACTCCAACCTCCT PRIMER_INTERNAL_3_SEQUENCE=GAAGACCACTGTGTGAAACTGG PRIMER_LEFT_3=88,20 PRIMER_RIGHT_3=179,20 PRIMER_INTERNAL_3=132,22 PRIMER_LEFT_3_TM=59.406 PRIMER_RIGHT_3_TM=60.261 PRIMER_INTERNAL_3_TM=59.664 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=11.2000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=92 PRIMER_PAIR_4_PENALTY=1.113647 PRIMER_LEFT_4_PENALTY=0.993838 PRIMER_RIGHT_4_PENALTY=0.119809 PRIMER_INTERNAL_4_PENALTY=2.335679 PRIMER_LEFT_4_SEQUENCE=CCCTGGCCATCATCTTACTT PRIMER_RIGHT_4_SEQUENCE=ACATGCTCACTCCAACCTCC PRIMER_INTERNAL_4_SEQUENCE=GAAGACCACTGTGTGAAACTGG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=180,20 PRIMER_INTERNAL_4=132,22 PRIMER_LEFT_4_TM=59.006 PRIMER_RIGHT_4_TM=60.120 PRIMER_INTERNAL_4_TM=59.664 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=5.7000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=82 = SEQUENCE_ID=MH996 SEQUENCE_TEMPLATE=GNTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTTTATAAGTAAGACATCTAAATTAGCAGATTTCATTCTTCCAAAATTAGAAAGTCATTAAGTTACAGATTTAGTTATGGAAGGATGGATGATGGCAGGTAAGTACTTAGGACTAAGTAGGAGCCACTCCTTAAGGAATCAATGTGCTTTTCAAATGTGTCATCTCATTTTATCCTGAACAACAGAAAATATTTTCTAGGTAAAGAAATTGAGGCTTAAAGAAGTAAAGTTATCTATAANCTAATAGTTTATAGACTCATTCCTGGTCTCTCACTAGATAATTTGCTGCGTGCTCAATTTTAGTCATGCAGGGAGTACTTACTAAACTTTACTTGGTATGCATATAATCAT SEQUENCE_INCLUDED_REGION=36,351 P3_COMMENT=CL is AAGACATCTAAATTAGCAGATTTCA P3_COMMENT=CR is CCTGCATGACTAAAATTGAGC P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.335435 PRIMER_LEFT_0_PENALTY=0.303491 PRIMER_RIGHT_0_PENALTY=3.031944 PRIMER_INTERNAL_0_PENALTY=1.998311 PRIMER_LEFT_0_SEQUENCE=AGGATGGATGATGGCAGGTA PRIMER_RIGHT_0_SEQUENCE=GACACATTTGAAAAGCACATTGA PRIMER_INTERNAL_0_SEQUENCE=AGGAGCCACTCCTTAAGGAA PRIMER_LEFT_0=117,20 PRIMER_RIGHT_0=196,23 PRIMER_INTERNAL_0=154,20 PRIMER_LEFT_0_TM=60.303 PRIMER_RIGHT_0_TM=60.032 PRIMER_INTERNAL_0_TM=58.002 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=34.783 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_INTERNAL_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=3.626232 PRIMER_LEFT_1_PENALTY=0.303491 PRIMER_RIGHT_1_PENALTY=3.322740 PRIMER_INTERNAL_1_PENALTY=1.815562 PRIMER_LEFT_1_SEQUENCE=AGGATGGATGATGGCAGGTA PRIMER_RIGHT_1_SEQUENCE=GATGACACATTTGAAAAGCACA PRIMER_INTERNAL_1_SEQUENCE=GGAGCCACTCCTTAAGGAATC PRIMER_LEFT_1=117,20 PRIMER_RIGHT_1=199,22 PRIMER_INTERNAL_1=155,21 PRIMER_LEFT_1_TM=60.303 PRIMER_RIGHT_1_TM=58.677 PRIMER_INTERNAL_1_TM=59.184 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=36.364 PRIMER_INTERNAL_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_INTERNAL_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=8.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=83 PRIMER_PAIR_2_PENALTY=3.635253 PRIMER_LEFT_2_PENALTY=0.303491 PRIMER_RIGHT_2_PENALTY=3.331761 PRIMER_INTERNAL_2_PENALTY=1.815562 PRIMER_LEFT_2_SEQUENCE=AGGATGGATGATGGCAGGTA PRIMER_RIGHT_2_SEQUENCE=AGATGACACATTTGAAAAGCACA PRIMER_INTERNAL_2_SEQUENCE=GGAGCCACTCCTTAAGGAATC PRIMER_LEFT_2=117,20 PRIMER_RIGHT_2=200,23 PRIMER_INTERNAL_2=155,21 PRIMER_LEFT_2_TM=60.303 PRIMER_RIGHT_2_TM=59.668 PRIMER_INTERNAL_2_TM=59.184 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=34.783 PRIMER_INTERNAL_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=10.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=8.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=84 PRIMER_PAIR_3_PENALTY=3.722889 PRIMER_LEFT_3_PENALTY=0.690945 PRIMER_RIGHT_3_PENALTY=3.031944 PRIMER_INTERNAL_3_PENALTY=1.998311 PRIMER_LEFT_3_SEQUENCE=GGATGGATGATGGCAGGTAA PRIMER_RIGHT_3_SEQUENCE=GACACATTTGAAAAGCACATTGA PRIMER_INTERNAL_3_SEQUENCE=AGGAGCCACTCCTTAAGGAA PRIMER_LEFT_3=118,20 PRIMER_RIGHT_3=196,23 PRIMER_INTERNAL_3=154,20 PRIMER_LEFT_3_TM=60.691 PRIMER_RIGHT_3_TM=60.032 PRIMER_INTERNAL_3_TM=58.002 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=34.783 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=10.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_INTERNAL_3_SELF_END=8.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=79 PRIMER_PAIR_4_PENALTY=3.966652 PRIMER_LEFT_4_PENALTY=0.303491 PRIMER_RIGHT_4_PENALTY=3.663161 PRIMER_INTERNAL_4_PENALTY=1.815562 PRIMER_LEFT_4_SEQUENCE=AGGATGGATGATGGCAGGTA PRIMER_RIGHT_4_SEQUENCE=TGAGATGACACATTTGAAAAGCA PRIMER_INTERNAL_4_SEQUENCE=GGAGCCACTCCTTAAGGAATC PRIMER_LEFT_4=117,20 PRIMER_RIGHT_4=202,23 PRIMER_INTERNAL_4=155,21 PRIMER_LEFT_4_TM=60.303 PRIMER_RIGHT_4_TM=60.663 PRIMER_INTERNAL_4_TM=59.184 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=34.783 PRIMER_INTERNAL_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_INTERNAL_4_SELF_ANY=10.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_INTERNAL_4_SELF_END=8.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=86 = SEQUENCE_ID=MH997 SEQUENCE_TEMPLATE=CTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTTATTTCTCTGGGCACTGCTTTCTTTGGGGGCAAACTTCCAGTATCACTGATACTAATATAAAAACCCTGTAAGTCTGCTTGCATTTTCAAGATTCAATATATATCCAGATTGTTTTCCCAGCAAAGAAAATTTTATTTCTCAAGATATAAAAAATAAATATTTAATTTCAGTTTCCTCAAAAGGAATATGAAATTTGTTAAAATGCAAATCCAGCTGTAACTTTTTTGGACTTGTCTTTTATTTCTTGCTTAGTAAAAGCACTATTAGTTTGGCATCATCAAATGAGTAGGGATGAG SEQUENCE_INCLUDED_REGION=35,301 P3_COMMENT=CL is CTCTTATTTCTCTGGGCACTG P3_COMMENT=CR is TCATCCCTACTCATTTGATGA P3_COMMENT=CM is _ P3_COMMENT=CC is 1,_ PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.068237 PRIMER_LEFT_0_PENALTY=0.067436 PRIMER_RIGHT_0_PENALTY=1.000801 PRIMER_INTERNAL_0_PENALTY=1.071885 PRIMER_LEFT_0_SEQUENCE=GGGGCAAACTTCCAGTATCA PRIMER_RIGHT_0_SEQUENCE=TTGCTGGGAAAACAATCTGG PRIMER_INTERNAL_0_SEQUENCE=AACCCTGTAAGTCTGCTTGCA PRIMER_LEFT_0=65,20 PRIMER_RIGHT_0=162,20 PRIMER_INTERNAL_0=101,21 PRIMER_LEFT_0_TM=59.933 PRIMER_RIGHT_0_TM=61.001 PRIMER_INTERNAL_0_TM=59.928 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=98 PRIMER_PAIR_1_PENALTY=1.083074 PRIMER_LEFT_1_PENALTY=0.067436 PRIMER_RIGHT_1_PENALTY=1.015638 PRIMER_INTERNAL_1_PENALTY=1.071885 PRIMER_LEFT_1_SEQUENCE=GGGGCAAACTTCCAGTATCA PRIMER_RIGHT_1_SEQUENCE=GCTGGGAAAACAATCTGGAT PRIMER_INTERNAL_1_SEQUENCE=AACCCTGTAAGTCTGCTTGCA PRIMER_LEFT_1=65,20 PRIMER_RIGHT_1=160,20 PRIMER_INTERNAL_1=101,21 PRIMER_LEFT_1_TM=59.933 PRIMER_RIGHT_1_TM=58.984 PRIMER_INTERNAL_1_TM=59.928 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=5.9000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=96 PRIMER_PAIR_2_PENALTY=1.205626 PRIMER_LEFT_2_PENALTY=0.133741 PRIMER_RIGHT_2_PENALTY=1.071885 PRIMER_INTERNAL_2_PENALTY=0.067436 PRIMER_LEFT_2_SEQUENCE=TCTCTGGGCACTGCTTTCTT PRIMER_RIGHT_2_SEQUENCE=TGCAAGCAGACTTACAGGGTT PRIMER_INTERNAL_2_SEQUENCE=GGGGCAAACTTCCAGTATCA PRIMER_LEFT_2=43,20 PRIMER_RIGHT_2=121,21 PRIMER_INTERNAL_2=65,20 PRIMER_LEFT_2_TM=60.134 PRIMER_RIGHT_2_TM=59.928 PRIMER_INTERNAL_2_TM=59.933 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=79 PRIMER_PAIR_3_PENALTY=1.205626 PRIMER_LEFT_3_PENALTY=0.133741 PRIMER_RIGHT_3_PENALTY=1.071885 PRIMER_INTERNAL_3_PENALTY=0.067436 PRIMER_LEFT_3_SEQUENCE=TTCTCTGGGCACTGCTTTCT PRIMER_RIGHT_3_SEQUENCE=TGCAAGCAGACTTACAGGGTT PRIMER_INTERNAL_3_SEQUENCE=GGGGCAAACTTCCAGTATCA PRIMER_LEFT_3=42,20 PRIMER_RIGHT_3=121,21 PRIMER_INTERNAL_3=65,20 PRIMER_LEFT_3_TM=60.134 PRIMER_RIGHT_3_TM=59.928 PRIMER_INTERNAL_3_TM=59.933 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=80 PRIMER_PAIR_4_PENALTY=1.322358 PRIMER_LEFT_4_PENALTY=0.067436 PRIMER_RIGHT_4_PENALTY=1.254922 PRIMER_INTERNAL_4_PENALTY=1.071885 PRIMER_LEFT_4_SEQUENCE=GGGGCAAACTTCCAGTATCA PRIMER_RIGHT_4_SEQUENCE=TTTGCTGGGAAAACAATCTG PRIMER_INTERNAL_4_SEQUENCE=AACCCTGTAAGTCTGCTTGCA PRIMER_LEFT_4=65,20 PRIMER_RIGHT_4=163,20 PRIMER_INTERNAL_4=101,21 PRIMER_LEFT_4_TM=59.933 PRIMER_RIGHT_4_TM=58.745 PRIMER_INTERNAL_4_TM=59.928 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=5.9000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=99 = primer3-2.3.6/test/primer_three_prime_distance_output0000664000077300007730000010201312231156245022130 0ustar ioanaioanaSEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1 - 11 possible pairs PRIMER_PRODUCT_SIZE_RANGE=100-500 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_NUM_RETURN=40 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT SEQUENCE_EXCLUDED_REGION=21,89 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1 SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC PRIMER_LEFT_NUM_RETURNED=11 PRIMER_RIGHT_NUM_RETURNED=11 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=11 PRIMER_PAIR_0_PENALTY=15.194088 PRIMER_LEFT_0_PENALTY=13.434466 PRIMER_RIGHT_0_PENALTY=1.759622 PRIMER_LEFT_0_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_0_SEQUENCE=GGGGATTTGCAAGAGACTTT PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=139,20 PRIMER_LEFT_0_TM=46.566 PRIMER_RIGHT_0_TM=58.240 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.8000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=140 PRIMER_PAIR_1_PENALTY=15.890689 PRIMER_LEFT_1_PENALTY=13.434466 PRIMER_RIGHT_1_PENALTY=2.456224 PRIMER_LEFT_1_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_1_SEQUENCE=GGATTTGCAAGAGACTTTGC PRIMER_LEFT_1=0,20 PRIMER_RIGHT_1=137,20 PRIMER_LEFT_1_TM=46.566 PRIMER_RIGHT_1_TM=57.544 PRIMER_LEFT_1_GC_PERCENT=25.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_LEFT_1_END_STABILITY=5.8000 PRIMER_RIGHT_1_END_STABILITY=8.8000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=138 PRIMER_PAIR_2_PENALTY=16.028766 PRIMER_LEFT_2_PENALTY=13.434466 PRIMER_RIGHT_2_PENALTY=2.594300 PRIMER_LEFT_2_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_2_SEQUENCE=TGCAAGAGACTTTGCTCAGA PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=132,20 PRIMER_LEFT_2_TM=46.566 PRIMER_RIGHT_2_TM=57.406 PRIMER_LEFT_2_GC_PERCENT=25.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=133 PRIMER_PAIR_3_PENALTY=16.041439 PRIMER_LEFT_3_PENALTY=13.434466 PRIMER_RIGHT_3_PENALTY=2.606973 PRIMER_LEFT_3_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_3_SEQUENCE=GGGATTTGCAAGAGACTTTG PRIMER_LEFT_3=0,20 PRIMER_RIGHT_3=138,20 PRIMER_LEFT_3_TM=46.566 PRIMER_RIGHT_3_TM=57.393 PRIMER_LEFT_3_GC_PERCENT=25.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=5.8000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=139 PRIMER_PAIR_4_PENALTY=16.088078 PRIMER_LEFT_4_PENALTY=13.434466 PRIMER_RIGHT_4_PENALTY=2.653612 PRIMER_LEFT_4_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_4_SEQUENCE=TTTGCAAGAGACTTTGCTCA PRIMER_LEFT_4=0,20 PRIMER_RIGHT_4=134,20 PRIMER_LEFT_4_TM=46.566 PRIMER_RIGHT_4_TM=57.346 PRIMER_LEFT_4_GC_PERCENT=25.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=135 PRIMER_PAIR_5_PENALTY=16.511012 PRIMER_LEFT_5_PENALTY=13.434466 PRIMER_RIGHT_5_PENALTY=3.076546 PRIMER_LEFT_5_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_5_SEQUENCE=TTGCAAGAGACTTTGCTCAG PRIMER_LEFT_5=0,20 PRIMER_RIGHT_5=133,20 PRIMER_LEFT_5_TM=46.566 PRIMER_RIGHT_5_TM=56.923 PRIMER_LEFT_5_GC_PERCENT=25.000 PRIMER_RIGHT_5_GC_PERCENT=45.000 PRIMER_LEFT_5_SELF_ANY=8.00 PRIMER_RIGHT_5_SELF_ANY=7.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_END_STABILITY=5.8000 PRIMER_RIGHT_5_END_STABILITY=6.7000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PAIR_5_PRODUCT_SIZE=134 PRIMER_PAIR_6_PENALTY=18.133199 PRIMER_LEFT_6_PENALTY=13.434466 PRIMER_RIGHT_6_PENALTY=4.698733 PRIMER_LEFT_6_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_6_SEQUENCE=GCAAGAGACTTTGCTCAGAC PRIMER_LEFT_6=0,20 PRIMER_RIGHT_6=131,20 PRIMER_LEFT_6_TM=46.566 PRIMER_RIGHT_6_TM=55.301 PRIMER_LEFT_6_GC_PERCENT=25.000 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_LEFT_6_SELF_ANY=8.00 PRIMER_RIGHT_6_SELF_ANY=7.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_END_STABILITY=5.8000 PRIMER_RIGHT_6_END_STABILITY=6.4000 PRIMER_PAIR_6_COMPL_ANY=6.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=132 PRIMER_PAIR_7_PENALTY=18.619053 PRIMER_LEFT_7_PENALTY=13.434466 PRIMER_RIGHT_7_PENALTY=5.184587 PRIMER_LEFT_7_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_7_SEQUENCE=ATTTGCAAGAGACTTTGCTC PRIMER_LEFT_7=0,20 PRIMER_RIGHT_7=135,20 PRIMER_LEFT_7_TM=46.566 PRIMER_RIGHT_7_TM=54.815 PRIMER_LEFT_7_GC_PERCENT=25.000 PRIMER_RIGHT_7_GC_PERCENT=40.000 PRIMER_LEFT_7_SELF_ANY=8.00 PRIMER_RIGHT_7_SELF_ANY=7.00 PRIMER_LEFT_7_SELF_END=3.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_END_STABILITY=5.8000 PRIMER_RIGHT_7_END_STABILITY=8.2000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PAIR_7_PRODUCT_SIZE=136 PRIMER_PAIR_8_PENALTY=18.619053 PRIMER_LEFT_8_PENALTY=13.434466 PRIMER_RIGHT_8_PENALTY=5.184587 PRIMER_LEFT_8_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_8_SEQUENCE=GATTTGCAAGAGACTTTGCT PRIMER_LEFT_8=0,20 PRIMER_RIGHT_8=136,20 PRIMER_LEFT_8_TM=46.566 PRIMER_RIGHT_8_TM=54.815 PRIMER_LEFT_8_GC_PERCENT=25.000 PRIMER_RIGHT_8_GC_PERCENT=40.000 PRIMER_LEFT_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_ANY=7.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=5.00 PRIMER_LEFT_8_END_STABILITY=5.8000 PRIMER_RIGHT_8_END_STABILITY=8.5000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=4.00 PRIMER_PAIR_8_PRODUCT_SIZE=137 PRIMER_PAIR_9_PENALTY=19.337053 PRIMER_LEFT_9_PENALTY=13.434466 PRIMER_RIGHT_9_PENALTY=5.902587 PRIMER_LEFT_9_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_9_SEQUENCE=CAAGAGACTTTGCTCAGACA PRIMER_LEFT_9=0,20 PRIMER_RIGHT_9=130,20 PRIMER_LEFT_9_TM=46.566 PRIMER_RIGHT_9_TM=54.097 PRIMER_LEFT_9_GC_PERCENT=25.000 PRIMER_RIGHT_9_GC_PERCENT=45.000 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=5.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_END_STABILITY=5.8000 PRIMER_RIGHT_9_END_STABILITY=6.4000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=4.00 PRIMER_PAIR_9_PRODUCT_SIZE=131 PRIMER_PAIR_10_PENALTY=21.454739 PRIMER_LEFT_10_PENALTY=13.434466 PRIMER_RIGHT_10_PENALTY=8.020273 PRIMER_LEFT_10_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_10_SEQUENCE=AAGAGACTTTGCTCAGACAC PRIMER_LEFT_10=0,20 PRIMER_RIGHT_10=129,20 PRIMER_LEFT_10_TM=46.566 PRIMER_RIGHT_10_TM=51.980 PRIMER_LEFT_10_GC_PERCENT=25.000 PRIMER_RIGHT_10_GC_PERCENT=45.000 PRIMER_LEFT_10_SELF_ANY=8.00 PRIMER_RIGHT_10_SELF_ANY=3.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=2.00 PRIMER_LEFT_10_END_STABILITY=5.8000 PRIMER_RIGHT_10_END_STABILITY=6.1000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=4.00 PRIMER_PAIR_10_PRODUCT_SIZE=130 = SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=0 - same results as for -1 SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT SEQUENCE_EXCLUDED_REGION=21,89 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=0 SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC PRIMER_LEFT_NUM_RETURNED=11 PRIMER_RIGHT_NUM_RETURNED=11 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=11 PRIMER_PAIR_0_PENALTY=15.194088 PRIMER_LEFT_0_PENALTY=13.434466 PRIMER_RIGHT_0_PENALTY=1.759622 PRIMER_LEFT_0_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_0_SEQUENCE=GGGGATTTGCAAGAGACTTT PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=139,20 PRIMER_LEFT_0_TM=46.566 PRIMER_RIGHT_0_TM=58.240 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.8000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=140 PRIMER_PAIR_1_PENALTY=15.890689 PRIMER_LEFT_1_PENALTY=13.434466 PRIMER_RIGHT_1_PENALTY=2.456224 PRIMER_LEFT_1_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_1_SEQUENCE=GGATTTGCAAGAGACTTTGC PRIMER_LEFT_1=0,20 PRIMER_RIGHT_1=137,20 PRIMER_LEFT_1_TM=46.566 PRIMER_RIGHT_1_TM=57.544 PRIMER_LEFT_1_GC_PERCENT=25.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_LEFT_1_END_STABILITY=5.8000 PRIMER_RIGHT_1_END_STABILITY=8.8000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=138 PRIMER_PAIR_2_PENALTY=16.028766 PRIMER_LEFT_2_PENALTY=13.434466 PRIMER_RIGHT_2_PENALTY=2.594300 PRIMER_LEFT_2_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_2_SEQUENCE=TGCAAGAGACTTTGCTCAGA PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=132,20 PRIMER_LEFT_2_TM=46.566 PRIMER_RIGHT_2_TM=57.406 PRIMER_LEFT_2_GC_PERCENT=25.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=133 PRIMER_PAIR_3_PENALTY=16.041439 PRIMER_LEFT_3_PENALTY=13.434466 PRIMER_RIGHT_3_PENALTY=2.606973 PRIMER_LEFT_3_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_3_SEQUENCE=GGGATTTGCAAGAGACTTTG PRIMER_LEFT_3=0,20 PRIMER_RIGHT_3=138,20 PRIMER_LEFT_3_TM=46.566 PRIMER_RIGHT_3_TM=57.393 PRIMER_LEFT_3_GC_PERCENT=25.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=5.8000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=139 PRIMER_PAIR_4_PENALTY=16.088078 PRIMER_LEFT_4_PENALTY=13.434466 PRIMER_RIGHT_4_PENALTY=2.653612 PRIMER_LEFT_4_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_4_SEQUENCE=TTTGCAAGAGACTTTGCTCA PRIMER_LEFT_4=0,20 PRIMER_RIGHT_4=134,20 PRIMER_LEFT_4_TM=46.566 PRIMER_RIGHT_4_TM=57.346 PRIMER_LEFT_4_GC_PERCENT=25.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=135 PRIMER_PAIR_5_PENALTY=16.511012 PRIMER_LEFT_5_PENALTY=13.434466 PRIMER_RIGHT_5_PENALTY=3.076546 PRIMER_LEFT_5_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_5_SEQUENCE=TTGCAAGAGACTTTGCTCAG PRIMER_LEFT_5=0,20 PRIMER_RIGHT_5=133,20 PRIMER_LEFT_5_TM=46.566 PRIMER_RIGHT_5_TM=56.923 PRIMER_LEFT_5_GC_PERCENT=25.000 PRIMER_RIGHT_5_GC_PERCENT=45.000 PRIMER_LEFT_5_SELF_ANY=8.00 PRIMER_RIGHT_5_SELF_ANY=7.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_END_STABILITY=5.8000 PRIMER_RIGHT_5_END_STABILITY=6.7000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PAIR_5_PRODUCT_SIZE=134 PRIMER_PAIR_6_PENALTY=18.133199 PRIMER_LEFT_6_PENALTY=13.434466 PRIMER_RIGHT_6_PENALTY=4.698733 PRIMER_LEFT_6_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_6_SEQUENCE=GCAAGAGACTTTGCTCAGAC PRIMER_LEFT_6=0,20 PRIMER_RIGHT_6=131,20 PRIMER_LEFT_6_TM=46.566 PRIMER_RIGHT_6_TM=55.301 PRIMER_LEFT_6_GC_PERCENT=25.000 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_LEFT_6_SELF_ANY=8.00 PRIMER_RIGHT_6_SELF_ANY=7.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_END_STABILITY=5.8000 PRIMER_RIGHT_6_END_STABILITY=6.4000 PRIMER_PAIR_6_COMPL_ANY=6.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=132 PRIMER_PAIR_7_PENALTY=18.619053 PRIMER_LEFT_7_PENALTY=13.434466 PRIMER_RIGHT_7_PENALTY=5.184587 PRIMER_LEFT_7_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_7_SEQUENCE=ATTTGCAAGAGACTTTGCTC PRIMER_LEFT_7=0,20 PRIMER_RIGHT_7=135,20 PRIMER_LEFT_7_TM=46.566 PRIMER_RIGHT_7_TM=54.815 PRIMER_LEFT_7_GC_PERCENT=25.000 PRIMER_RIGHT_7_GC_PERCENT=40.000 PRIMER_LEFT_7_SELF_ANY=8.00 PRIMER_RIGHT_7_SELF_ANY=7.00 PRIMER_LEFT_7_SELF_END=3.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_END_STABILITY=5.8000 PRIMER_RIGHT_7_END_STABILITY=8.2000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PAIR_7_PRODUCT_SIZE=136 PRIMER_PAIR_8_PENALTY=18.619053 PRIMER_LEFT_8_PENALTY=13.434466 PRIMER_RIGHT_8_PENALTY=5.184587 PRIMER_LEFT_8_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_8_SEQUENCE=GATTTGCAAGAGACTTTGCT PRIMER_LEFT_8=0,20 PRIMER_RIGHT_8=136,20 PRIMER_LEFT_8_TM=46.566 PRIMER_RIGHT_8_TM=54.815 PRIMER_LEFT_8_GC_PERCENT=25.000 PRIMER_RIGHT_8_GC_PERCENT=40.000 PRIMER_LEFT_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_ANY=7.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=5.00 PRIMER_LEFT_8_END_STABILITY=5.8000 PRIMER_RIGHT_8_END_STABILITY=8.5000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=4.00 PRIMER_PAIR_8_PRODUCT_SIZE=137 PRIMER_PAIR_9_PENALTY=19.337053 PRIMER_LEFT_9_PENALTY=13.434466 PRIMER_RIGHT_9_PENALTY=5.902587 PRIMER_LEFT_9_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_9_SEQUENCE=CAAGAGACTTTGCTCAGACA PRIMER_LEFT_9=0,20 PRIMER_RIGHT_9=130,20 PRIMER_LEFT_9_TM=46.566 PRIMER_RIGHT_9_TM=54.097 PRIMER_LEFT_9_GC_PERCENT=25.000 PRIMER_RIGHT_9_GC_PERCENT=45.000 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=5.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_END_STABILITY=5.8000 PRIMER_RIGHT_9_END_STABILITY=6.4000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=4.00 PRIMER_PAIR_9_PRODUCT_SIZE=131 PRIMER_PAIR_10_PENALTY=21.454739 PRIMER_LEFT_10_PENALTY=13.434466 PRIMER_RIGHT_10_PENALTY=8.020273 PRIMER_LEFT_10_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_10_SEQUENCE=AAGAGACTTTGCTCAGACAC PRIMER_LEFT_10=0,20 PRIMER_RIGHT_10=129,20 PRIMER_LEFT_10_TM=46.566 PRIMER_RIGHT_10_TM=51.980 PRIMER_LEFT_10_GC_PERCENT=25.000 PRIMER_RIGHT_10_GC_PERCENT=45.000 PRIMER_LEFT_10_SELF_ANY=8.00 PRIMER_RIGHT_10_SELF_ANY=3.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=2.00 PRIMER_LEFT_10_END_STABILITY=5.8000 PRIMER_RIGHT_10_END_STABILITY=6.1000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=4.00 PRIMER_PAIR_10_PRODUCT_SIZE=130 = SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 - same results as for 0 and -1 SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT SEQUENCE_EXCLUDED_REGION=21,89 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC PRIMER_LEFT_NUM_RETURNED=11 PRIMER_RIGHT_NUM_RETURNED=11 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=11 PRIMER_PAIR_0_PENALTY=15.194088 PRIMER_LEFT_0_PENALTY=13.434466 PRIMER_RIGHT_0_PENALTY=1.759622 PRIMER_LEFT_0_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_0_SEQUENCE=GGGGATTTGCAAGAGACTTT PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=139,20 PRIMER_LEFT_0_TM=46.566 PRIMER_RIGHT_0_TM=58.240 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.8000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=140 PRIMER_PAIR_1_PENALTY=15.890689 PRIMER_LEFT_1_PENALTY=13.434466 PRIMER_RIGHT_1_PENALTY=2.456224 PRIMER_LEFT_1_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_1_SEQUENCE=GGATTTGCAAGAGACTTTGC PRIMER_LEFT_1=0,20 PRIMER_RIGHT_1=137,20 PRIMER_LEFT_1_TM=46.566 PRIMER_RIGHT_1_TM=57.544 PRIMER_LEFT_1_GC_PERCENT=25.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_LEFT_1_END_STABILITY=5.8000 PRIMER_RIGHT_1_END_STABILITY=8.8000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=138 PRIMER_PAIR_2_PENALTY=16.028766 PRIMER_LEFT_2_PENALTY=13.434466 PRIMER_RIGHT_2_PENALTY=2.594300 PRIMER_LEFT_2_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_2_SEQUENCE=TGCAAGAGACTTTGCTCAGA PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=132,20 PRIMER_LEFT_2_TM=46.566 PRIMER_RIGHT_2_TM=57.406 PRIMER_LEFT_2_GC_PERCENT=25.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=133 PRIMER_PAIR_3_PENALTY=16.041439 PRIMER_LEFT_3_PENALTY=13.434466 PRIMER_RIGHT_3_PENALTY=2.606973 PRIMER_LEFT_3_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_3_SEQUENCE=GGGATTTGCAAGAGACTTTG PRIMER_LEFT_3=0,20 PRIMER_RIGHT_3=138,20 PRIMER_LEFT_3_TM=46.566 PRIMER_RIGHT_3_TM=57.393 PRIMER_LEFT_3_GC_PERCENT=25.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=5.8000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=139 PRIMER_PAIR_4_PENALTY=16.088078 PRIMER_LEFT_4_PENALTY=13.434466 PRIMER_RIGHT_4_PENALTY=2.653612 PRIMER_LEFT_4_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_4_SEQUENCE=TTTGCAAGAGACTTTGCTCA PRIMER_LEFT_4=0,20 PRIMER_RIGHT_4=134,20 PRIMER_LEFT_4_TM=46.566 PRIMER_RIGHT_4_TM=57.346 PRIMER_LEFT_4_GC_PERCENT=25.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=135 PRIMER_PAIR_5_PENALTY=16.511012 PRIMER_LEFT_5_PENALTY=13.434466 PRIMER_RIGHT_5_PENALTY=3.076546 PRIMER_LEFT_5_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_5_SEQUENCE=TTGCAAGAGACTTTGCTCAG PRIMER_LEFT_5=0,20 PRIMER_RIGHT_5=133,20 PRIMER_LEFT_5_TM=46.566 PRIMER_RIGHT_5_TM=56.923 PRIMER_LEFT_5_GC_PERCENT=25.000 PRIMER_RIGHT_5_GC_PERCENT=45.000 PRIMER_LEFT_5_SELF_ANY=8.00 PRIMER_RIGHT_5_SELF_ANY=7.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_END_STABILITY=5.8000 PRIMER_RIGHT_5_END_STABILITY=6.7000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PAIR_5_PRODUCT_SIZE=134 PRIMER_PAIR_6_PENALTY=18.133199 PRIMER_LEFT_6_PENALTY=13.434466 PRIMER_RIGHT_6_PENALTY=4.698733 PRIMER_LEFT_6_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_6_SEQUENCE=GCAAGAGACTTTGCTCAGAC PRIMER_LEFT_6=0,20 PRIMER_RIGHT_6=131,20 PRIMER_LEFT_6_TM=46.566 PRIMER_RIGHT_6_TM=55.301 PRIMER_LEFT_6_GC_PERCENT=25.000 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_LEFT_6_SELF_ANY=8.00 PRIMER_RIGHT_6_SELF_ANY=7.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_END_STABILITY=5.8000 PRIMER_RIGHT_6_END_STABILITY=6.4000 PRIMER_PAIR_6_COMPL_ANY=6.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=132 PRIMER_PAIR_7_PENALTY=18.619053 PRIMER_LEFT_7_PENALTY=13.434466 PRIMER_RIGHT_7_PENALTY=5.184587 PRIMER_LEFT_7_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_7_SEQUENCE=ATTTGCAAGAGACTTTGCTC PRIMER_LEFT_7=0,20 PRIMER_RIGHT_7=135,20 PRIMER_LEFT_7_TM=46.566 PRIMER_RIGHT_7_TM=54.815 PRIMER_LEFT_7_GC_PERCENT=25.000 PRIMER_RIGHT_7_GC_PERCENT=40.000 PRIMER_LEFT_7_SELF_ANY=8.00 PRIMER_RIGHT_7_SELF_ANY=7.00 PRIMER_LEFT_7_SELF_END=3.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_END_STABILITY=5.8000 PRIMER_RIGHT_7_END_STABILITY=8.2000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PAIR_7_PRODUCT_SIZE=136 PRIMER_PAIR_8_PENALTY=18.619053 PRIMER_LEFT_8_PENALTY=13.434466 PRIMER_RIGHT_8_PENALTY=5.184587 PRIMER_LEFT_8_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_8_SEQUENCE=GATTTGCAAGAGACTTTGCT PRIMER_LEFT_8=0,20 PRIMER_RIGHT_8=136,20 PRIMER_LEFT_8_TM=46.566 PRIMER_RIGHT_8_TM=54.815 PRIMER_LEFT_8_GC_PERCENT=25.000 PRIMER_RIGHT_8_GC_PERCENT=40.000 PRIMER_LEFT_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_ANY=7.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=5.00 PRIMER_LEFT_8_END_STABILITY=5.8000 PRIMER_RIGHT_8_END_STABILITY=8.5000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=4.00 PRIMER_PAIR_8_PRODUCT_SIZE=137 PRIMER_PAIR_9_PENALTY=19.337053 PRIMER_LEFT_9_PENALTY=13.434466 PRIMER_RIGHT_9_PENALTY=5.902587 PRIMER_LEFT_9_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_9_SEQUENCE=CAAGAGACTTTGCTCAGACA PRIMER_LEFT_9=0,20 PRIMER_RIGHT_9=130,20 PRIMER_LEFT_9_TM=46.566 PRIMER_RIGHT_9_TM=54.097 PRIMER_LEFT_9_GC_PERCENT=25.000 PRIMER_RIGHT_9_GC_PERCENT=45.000 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=5.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_END_STABILITY=5.8000 PRIMER_RIGHT_9_END_STABILITY=6.4000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=4.00 PRIMER_PAIR_9_PRODUCT_SIZE=131 PRIMER_PAIR_10_PENALTY=21.454739 PRIMER_LEFT_10_PENALTY=13.434466 PRIMER_RIGHT_10_PENALTY=8.020273 PRIMER_LEFT_10_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_10_SEQUENCE=AAGAGACTTTGCTCAGACAC PRIMER_LEFT_10=0,20 PRIMER_RIGHT_10=129,20 PRIMER_LEFT_10_TM=46.566 PRIMER_RIGHT_10_TM=51.980 PRIMER_LEFT_10_GC_PERCENT=25.000 PRIMER_RIGHT_10_GC_PERCENT=45.000 PRIMER_LEFT_10_SELF_ANY=8.00 PRIMER_RIGHT_10_SELF_ANY=3.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=2.00 PRIMER_LEFT_10_END_STABILITY=5.8000 PRIMER_RIGHT_10_END_STABILITY=6.1000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=4.00 PRIMER_PAIR_10_PRODUCT_SIZE=130 = SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=2 - 5 pairs SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT SEQUENCE_EXCLUDED_REGION=21,89 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=2 SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=15.194088 PRIMER_LEFT_0_PENALTY=13.434466 PRIMER_RIGHT_0_PENALTY=1.759622 PRIMER_LEFT_0_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_0_SEQUENCE=GGGGATTTGCAAGAGACTTT PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=139,20 PRIMER_LEFT_0_TM=46.566 PRIMER_RIGHT_0_TM=58.240 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.8000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=140 PRIMER_PAIR_1_PENALTY=15.890689 PRIMER_LEFT_1_PENALTY=13.434466 PRIMER_RIGHT_1_PENALTY=2.456224 PRIMER_LEFT_1_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_1_SEQUENCE=GGATTTGCAAGAGACTTTGC PRIMER_LEFT_1=0,20 PRIMER_RIGHT_1=137,20 PRIMER_LEFT_1_TM=46.566 PRIMER_RIGHT_1_TM=57.544 PRIMER_LEFT_1_GC_PERCENT=25.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=7.00 PRIMER_LEFT_1_END_STABILITY=5.8000 PRIMER_RIGHT_1_END_STABILITY=8.8000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=138 PRIMER_PAIR_2_PENALTY=16.028766 PRIMER_LEFT_2_PENALTY=13.434466 PRIMER_RIGHT_2_PENALTY=2.594300 PRIMER_LEFT_2_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_2_SEQUENCE=TGCAAGAGACTTTGCTCAGA PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=132,20 PRIMER_LEFT_2_TM=46.566 PRIMER_RIGHT_2_TM=57.406 PRIMER_LEFT_2_GC_PERCENT=25.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=133 PRIMER_PAIR_3_PENALTY=16.088078 PRIMER_LEFT_3_PENALTY=13.434466 PRIMER_RIGHT_3_PENALTY=2.653612 PRIMER_LEFT_3_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_3_SEQUENCE=TTTGCAAGAGACTTTGCTCA PRIMER_LEFT_3=0,20 PRIMER_RIGHT_3=134,20 PRIMER_LEFT_3_TM=46.566 PRIMER_RIGHT_3_TM=57.346 PRIMER_LEFT_3_GC_PERCENT=25.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=5.8000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=135 PRIMER_PAIR_4_PENALTY=19.337053 PRIMER_LEFT_4_PENALTY=13.434466 PRIMER_RIGHT_4_PENALTY=5.902587 PRIMER_LEFT_4_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_4_SEQUENCE=CAAGAGACTTTGCTCAGACA PRIMER_LEFT_4=0,20 PRIMER_RIGHT_4=130,20 PRIMER_LEFT_4_TM=46.566 PRIMER_RIGHT_4_TM=54.097 PRIMER_LEFT_4_GC_PERCENT=25.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=131 = SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=3 - 4 pairs SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT SEQUENCE_EXCLUDED_REGION=21,89 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=3 SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC PRIMER_LEFT_NUM_RETURNED=4 PRIMER_RIGHT_NUM_RETURNED=4 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=4 PRIMER_PAIR_0_PENALTY=15.194088 PRIMER_LEFT_0_PENALTY=13.434466 PRIMER_RIGHT_0_PENALTY=1.759622 PRIMER_LEFT_0_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_0_SEQUENCE=GGGGATTTGCAAGAGACTTT PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=139,20 PRIMER_LEFT_0_TM=46.566 PRIMER_RIGHT_0_TM=58.240 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.8000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=140 PRIMER_PAIR_1_PENALTY=16.028766 PRIMER_LEFT_1_PENALTY=13.434466 PRIMER_RIGHT_1_PENALTY=2.594300 PRIMER_LEFT_1_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_1_SEQUENCE=TGCAAGAGACTTTGCTCAGA PRIMER_LEFT_1=0,20 PRIMER_RIGHT_1=132,20 PRIMER_LEFT_1_TM=46.566 PRIMER_RIGHT_1_TM=57.406 PRIMER_LEFT_1_GC_PERCENT=25.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=5.8000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=133 PRIMER_PAIR_2_PENALTY=18.619053 PRIMER_LEFT_2_PENALTY=13.434466 PRIMER_RIGHT_2_PENALTY=5.184587 PRIMER_LEFT_2_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_2_SEQUENCE=ATTTGCAAGAGACTTTGCTC PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=135,20 PRIMER_LEFT_2_TM=46.566 PRIMER_RIGHT_2_TM=54.815 PRIMER_LEFT_2_GC_PERCENT=25.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=136 PRIMER_PAIR_3_PENALTY=21.454739 PRIMER_LEFT_3_PENALTY=13.434466 PRIMER_RIGHT_3_PENALTY=8.020273 PRIMER_LEFT_3_SEQUENCE=ATGAATTAGCAAGTCATTAT PRIMER_RIGHT_3_SEQUENCE=AAGAGACTTTGCTCAGACAC PRIMER_LEFT_3=0,20 PRIMER_RIGHT_3=129,20 PRIMER_LEFT_3_TM=46.566 PRIMER_RIGHT_3_TM=51.980 PRIMER_LEFT_3_GC_PERCENT=25.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=5.8000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=130 = SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1 - 11 possible pairs SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC SEQUENCE_EXCLUDED_REGION=31,89 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1 SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=16.938641 PRIMER_LEFT_0_PENALTY=9.244200 PRIMER_RIGHT_0_PENALTY=7.694441 PRIMER_LEFT_0_SEQUENCE=TGATGAATTAGCAAGTCATT PRIMER_RIGHT_0_SEQUENCE=TTTCACTTTCTAATGAAGGC PRIMER_LEFT_0=8,20 PRIMER_RIGHT_0=139,20 PRIMER_LEFT_0_TM=50.756 PRIMER_RIGHT_0_TM=52.306 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=35.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=132 = SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=0 - same results as for -1 SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC SEQUENCE_EXCLUDED_REGION=31,89 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=0 SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=16.938641 PRIMER_LEFT_0_PENALTY=9.244200 PRIMER_RIGHT_0_PENALTY=7.694441 PRIMER_LEFT_0_SEQUENCE=TGATGAATTAGCAAGTCATT PRIMER_RIGHT_0_SEQUENCE=TTTCACTTTCTAATGAAGGC PRIMER_LEFT_0=8,20 PRIMER_RIGHT_0=139,20 PRIMER_LEFT_0_TM=50.756 PRIMER_RIGHT_0_TM=52.306 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=35.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=132 = SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 - same results as for 0 and -1 SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC SEQUENCE_EXCLUDED_REGION=31,89 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=16.938641 PRIMER_LEFT_0_PENALTY=9.244200 PRIMER_RIGHT_0_PENALTY=7.694441 PRIMER_LEFT_0_SEQUENCE=TGATGAATTAGCAAGTCATT PRIMER_RIGHT_0_SEQUENCE=TTTCACTTTCTAATGAAGGC PRIMER_LEFT_0=8,20 PRIMER_RIGHT_0=139,20 PRIMER_LEFT_0_TM=50.756 PRIMER_RIGHT_0_TM=52.306 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=35.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=132 = SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=2 - 5 pairs SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC SEQUENCE_EXCLUDED_REGION=31,89 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=2 SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=16.938641 PRIMER_LEFT_0_PENALTY=9.244200 PRIMER_RIGHT_0_PENALTY=7.694441 PRIMER_LEFT_0_SEQUENCE=TGATGAATTAGCAAGTCATT PRIMER_RIGHT_0_SEQUENCE=TTTCACTTTCTAATGAAGGC PRIMER_LEFT_0=8,20 PRIMER_RIGHT_0=139,20 PRIMER_LEFT_0_TM=50.756 PRIMER_RIGHT_0_TM=52.306 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=35.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=132 = SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=3 - 3 pairs SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC SEQUENCE_EXCLUDED_REGION=31,89 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=3 SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=16.938641 PRIMER_LEFT_0_PENALTY=9.244200 PRIMER_RIGHT_0_PENALTY=7.694441 PRIMER_LEFT_0_SEQUENCE=TGATGAATTAGCAAGTCATT PRIMER_RIGHT_0_SEQUENCE=TTTCACTTTCTAATGAAGGC PRIMER_LEFT_0=8,20 PRIMER_RIGHT_0=139,20 PRIMER_LEFT_0_TM=50.756 PRIMER_RIGHT_0_TM=52.306 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=35.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=8.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=132 = primer3-2.3.6/test/primer_tm_lc_masking_input0000664000077300007730000000562612231156245020375 0ustar ioanaioanaPRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=0 PRIMER_LOWERCASE_MASKING=0 PRIMER_NUM_RETURN=1 PRIMER_TASK=pick_left_only PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_SIZE=10 PRIMER_MAX_SIZE=10 PRIMER_OPT_SIZE=10 PRIMER_MIN_TM=0 PRIMER_MAX_TM=70 P3_COMMENT=nomasking_Breslauer_Schildkraut SEQUENCE_ID=000 SEQUENCE_TEMPLATE=TCCTTGTGAT = PRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=1 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_Breslauer_SantaLucia SEQUENCE_ID=001 SEQUENCE_TEMPLATE=TCCTTGTGAT = PRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=2 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_Breslauer_Owczarzy SEQUENCE_ID=002 SEQUENCE_TEMPLATE=TCCTTGTGAT = PRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=0 PRIMER_LOWERCASE_MASKING=1 P3_COMMENT=masking_Breslauer_Schildkraut P3_COMMENT=Should fail SEQUENCE_ID=100 SEQUENCE_TEMPLATE=TCCTTGTGAt = PRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=1 PRIMER_LOWERCASE_MASKING=1 SEQUENCE_ID=101 P3_COMMENT=masking_Breslauer_SantaLucia P3_COMMENT=Should succeed SEQUENCE_TEMPLATE=TCCTTGTGaT = PRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=2 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=masking_Breslauer_Owczarzy P3_COMMENT=Should succeed SEQUENCE_ID=102 SEQUENCE_TEMPLATE=tccttgtgat = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=0 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_SantaLucia_Schildkraut SEQUENCE_ID=010 SEQUENCE_TEMPLATE=TCCTTGTGAT = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_SantaLucia_SantaLucia SEQUENCE_ID=011 SEQUENCE_TEMPLATE=TCCTTGTGAG = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=2 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_SantaLucia_Owczarzy SEQUENCE_ID=012 SEQUENCE_TEMPLATE=GCCTTGTGAG = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=0 PRIMER_LOWERCASE_MASKING=1 P3_COMMENT=masking_SantaLucia_Schildkraut P3_COMMENT=Should fail SEQUENCE_ID=110 SEQUENCE_TEMPLATE=TCCTTGTGAt = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_LOWERCASE_MASKING=1 P3_COMMENT=masking_SantaLucia_SantaLucia P3_COMMENT=Should succeed SEQUENCE_ID=111 SEQUENCE_TEMPLATE=TCCTTGTGaT = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=2 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_SantaLucia_Owczarzy P3_COMMENT=Should succeed SEQUENCE_ID=112 SEQUENCE_TEMPLATE=tccttgtgat = SEQUENCE_ID=masking_001 PRIMER_LOWERCASE_MASKING=1 PRIMER_PRODUCT_SIZE_RANGE=40-300 PRIMER_TASK=pick_pcr_primers PRIMER_MAX_SIZE=20 PRIMER_MIN_SIZE=20 PRIMER_OPT_SIZE=20 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAT = SEQUENCE_ID=masking_002 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNaATCACACCTGGGTACTAAT = SEQUENCE_ID=masking_003 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAt = SEQUENCE_ID=masking_004 SEQUENCE_TEMPLATE=gCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAT = SEQUENCE_ID=masking_005 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACaNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAT = primer3-2.3.6/test/primer1_th_list_last/0000775000077300007730000000000012231156372017166 5ustar ioanaioanaprimer3-2.3.6/test/primer1_th_list_last/SP01-15.txt.rev0000664000077300007730000000573712231156243021441 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TTTTTTAAACGACGGCCAGT 283 20 0 40.00 57.391 0.12 0.12 0.00 2.609 1 ATTTTTTAAACGACGGCCAGT 284 21 0 38.10 57.921 0.12 0.12 0.00 3.079 2 TTTTAAACGACGGCCAGTG 281 19 0 47.37 57.368 1.94 0.00 0.00 3.632 3 TTTTTAAACGACGGCCAGTG 282 20 0 45.00 58.136 1.94 0.00 0.00 1.864 4 TTTTTTAAACGACGGCCAGTG 283 21 0 42.86 58.833 1.94 0.00 0.00 2.167 5 ATTTTTTAAACGACGGCCAGTG 284 22 0 40.91 59.275 1.94 0.00 0.00 2.725 6 TTAAACGACGGCCAGTGA 279 18 0 50.00 57.405 5.47 0.00 0.00 4.595 7 TTTAAACGACGGCCAGTGA 280 19 0 47.37 58.222 5.47 0.00 0.00 2.778 8 TTTTAAACGACGGCCAGTGA 281 20 0 45.00 58.960 5.47 0.00 0.00 1.040 9 TTTTTAAACGACGGCCAGTGA 282 21 0 42.86 59.629 5.47 0.00 0.00 1.371 10 TTTTTTAAACGACGGCCAGTGA 283 22 0 40.91 60.239 5.47 0.00 0.00 2.239 11 ATTTTTTAAACGACGGCCAGTGA 284 23 0 39.13 60.612 5.47 0.00 0.00 3.612 12 TAAACGACGGCCAGTGAG 278 18 0 55.56 57.909 5.47 3.36 0.00 4.091 13 TTAAACGACGGCCAGTGAG 279 19 0 52.63 58.690 5.47 3.36 0.00 2.310 14 TTTAAACGACGGCCAGTGAG 280 20 0 50.00 59.397 5.47 3.36 0.00 0.603 15 TTTTAAACGACGGCCAGTGAG 281 21 0 47.62 60.038 5.47 3.36 0.00 1.038 16 TTTTTAAACGACGGCCAGTGAG 282 22 0 45.45 60.623 5.47 3.36 0.00 2.623 17 TTTTTTAAACGACGGCCAGTGAG 283 23 0 43.48 61.159 5.47 3.36 0.00 4.159 18 ATTTTTTAAACGACGGCCAGTGAG 284 24 0 41.67 61.479 5.47 3.36 0.00 5.479 19 AAACGACGGCCAGTGAGC 277 18 0 61.11 61.557 5.47 0.00 0.00 3.557 20 TAAACGACGGCCAGTGAGC 278 19 0 57.89 61.271 5.47 0.00 0.00 2.271 21 TTAAACGACGGCCAGTGAGC 279 20 0 55.00 61.851 5.47 0.00 0.00 1.851 22 TTTAAACGACGGCCAGTGAGC 280 21 0 52.38 62.377 5.47 0.00 0.00 3.377 23 TTTTAAACGACGGCCAGTGAGC 281 22 0 50.00 62.858 5.47 0.00 0.00 4.858 24 CCAGTGAGCGCGCGTAAT 268 18 0 61.11 61.764 6.06 0.00 0.00 3.764 25 CAGTGAGCGCGCGTAATA 267 18 0 55.56 58.877 6.06 0.00 0.00 3.123 26 CCAGTGAGCGCGCGTAATA 268 19 0 57.89 61.473 6.06 0.00 0.00 2.473 27 CCAGAGATCCACCGTCGG 228 18 0 66.67 60.088 10.98 10.98 0.00 2.088 28 CAGAGATCCACCGTCGGT 227 18 0 61.11 59.298 17.25 15.25 36.10 2.702 29 CCAGAGATCCACCGTCGGT 228 19 0 63.16 61.972 17.25 15.25 36.10 2.972 30 AGAGATCCACCGTCGGTA 226 18 0 55.56 57.512 18.13 2.72 32.96 4.488 31 CAGAGATCCACCGTCGGTA 227 19 0 57.89 59.119 18.13 2.72 32.96 1.881 32 CCAGAGATCCACCGTCGGTA 228 20 0 60.00 61.674 18.13 2.72 32.96 1.674 primer3-2.3.6/test/primer1_th_list_last/targets_test.for0000664000077300007730000000031012231156243022375 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity primer3-2.3.6/test/primer1_th_list_last/SP01-20.txt.for0000664000077300007730000000104012231156243021406 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 AATCTGGGGCCGTTGGGG 101 18 0 66.67 62.999 0.00 0.00 39.58 4.999 1 AAATCTGGGGCCGTTGGG 100 18 0 61.11 60.890 0.00 0.00 0.00 2.890 2 GAAATCTGGGGCCGTTGGG 99 19 0 63.16 61.971 0.00 0.00 0.00 2.971 3 GAAATCTGGGGCCGTTGG 99 18 0 61.11 59.299 0.00 0.00 0.00 2.701 primer3-2.3.6/test/primer1_th_list_last/included_test.rev0000664000077300007730000011263312231156243022535 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 ACACACACACACAACACACA 179 20 0 45.00 59.314 0.00 0.00 0.00 0.686 1 ACACACACACAACACACAGT 177 20 0 45.00 59.025 0.00 0.00 0.00 0.975 2 ACACAACAGACACACACACAC 188 21 0 47.62 60.105 0.00 0.00 0.00 1.105 3 ACAGACACACACACACAACAC 183 21 0 47.62 60.105 0.00 0.00 0.00 1.105 4 AGACACACACACACAACACAC 181 21 0 47.62 60.105 0.00 0.00 0.00 1.105 5 ACACACACACAACACACAGTC 177 21 0 47.62 60.105 0.00 0.00 0.00 1.105 6 CACAACAGACACACACACACA 187 21 0 47.62 60.105 0.00 0.00 0.00 1.105 7 CAGACACACACACACAACACA 182 21 0 47.62 60.105 0.00 0.00 0.00 1.105 8 CACACACACAACACACAGTCA 176 21 0 47.62 60.105 0.00 0.00 0.00 1.105 9 ACACAACAGACACACACACA 188 20 0 45.00 58.738 0.00 0.00 0.00 1.262 10 ACAACAGACACACACACACA 186 20 0 45.00 58.738 0.00 0.00 0.00 1.262 11 ACAGACACACACACACAACA 183 20 0 45.00 58.738 0.00 0.00 0.00 1.262 12 AGACACACACACACAACACA 181 20 0 45.00 58.738 0.00 0.00 0.00 1.262 13 ACACACACAACACACAGTCA 175 20 0 45.00 58.738 0.00 0.00 0.00 1.262 14 GACACACACACACAACACAC 180 20 0 50.00 58.645 0.00 0.00 0.00 1.355 15 CACACACACACAACACACAG 178 20 0 50.00 58.642 0.00 0.00 0.00 1.358 16 GACACACACACACAACACACA 180 21 0 47.62 60.374 0.00 0.00 0.00 1.374 17 ACACACACACACAACACACAG 179 21 0 47.62 60.374 0.00 0.00 0.00 1.374 18 CACACACACACAACACACAGT 178 21 0 47.62 60.374 0.00 0.00 0.00 1.374 19 ACAACAGACACACACACACAA 186 21 0 42.86 59.421 0.00 0.00 0.00 1.579 20 AACAGACACACACACACAACA 184 21 0 42.86 59.421 0.00 0.00 0.00 1.579 21 ACACACACAACACACAGTCAA 175 21 0 42.86 59.421 0.00 0.00 0.00 1.579 22 CACAACAGACACACACACAC 187 20 0 50.00 58.362 0.00 0.00 0.00 1.638 23 CAGACACACACACACAACAC 182 20 0 50.00 58.362 0.00 0.00 0.00 1.638 24 CACACACACAACACACAGTC 176 20 0 50.00 58.362 0.00 0.00 0.00 1.638 25 CAACAGACACACACACACAAC 185 21 0 47.62 59.044 0.00 0.00 0.00 1.956 26 ACAACACACAGTCAAGAAGCT 169 21 0 42.86 58.935 0.00 0.00 0.00 2.065 27 TACACAACAGACACACACACAC 189 22 0 45.45 59.916 0.00 0.00 0.00 2.084 28 ACACACAACACACAGTCAAGA 173 21 0 42.86 58.870 0.00 0.00 0.00 2.130 29 CACAACACACAGTCAAGAAGC 170 21 0 47.62 58.840 0.00 0.00 0.00 2.160 30 CACACACAACACACAGTCAAGA 174 22 0 45.45 60.174 0.00 0.00 0.00 2.174 31 CACACACAACACACAGTCAAG 174 21 0 47.62 58.774 0.00 0.00 0.00 2.226 32 CACAACACACAGTCAAGAAGCT 170 22 0 45.45 60.239 0.00 0.00 0.00 2.239 33 CAACAGACACACACACACAA 185 20 0 45.00 57.629 0.00 0.00 0.00 2.371 34 CACACACAACACACAGTCAA 174 20 0 45.00 57.629 0.00 0.00 0.00 2.371 35 AACAGACACACACACACAAC 184 20 0 45.00 57.627 0.00 0.00 0.00 2.373 36 TACACAACAGACACACACACA 189 21 0 42.86 58.599 0.00 0.00 0.00 2.401 37 ACACACACAACACACAGTCAAG 175 22 0 45.45 60.432 0.00 0.00 0.00 2.432 38 ACACACAACACACAGTCAAGAA 173 22 0 40.91 59.519 0.00 0.00 0.00 2.481 39 ACACAACACACAGTCAAGAAGC 171 22 0 45.45 60.497 0.00 0.00 0.00 2.497 40 ACAACACACAGTCAAGAAGC 169 20 0 45.00 57.405 0.00 0.00 0.00 2.595 41 ACACACAACACACAGTCAAG 173 20 0 45.00 57.337 0.00 0.00 0.00 2.663 42 ACACACACACACAACATACACT 243 22 0 40.91 59.322 0.00 0.00 0.00 2.678 43 CACAACAGACACACACACACAA 187 22 0 45.45 60.688 0.00 0.00 0.00 2.688 44 CAACAGACACACACACACAACA 185 22 0 45.45 60.688 0.00 0.00 0.00 2.688 45 CACACACACAACACACAGTCAA 176 22 0 45.45 60.688 0.00 0.00 0.00 2.688 46 ACAACAGACACACACACACAAC 186 22 0 45.45 60.689 0.00 0.00 0.00 2.689 47 AACAGACACACACACACAACAC 184 22 0 45.45 60.689 0.00 0.00 0.00 2.689 48 CAACACACAGTCAAGAAGCT 168 20 0 45.00 57.112 0.00 0.00 0.00 2.888 49 ACACACAGTCAAGAAGCTACA 166 21 0 42.86 58.106 0.00 0.00 0.00 2.894 50 ATACACAACAGACACACACACA 190 22 0 40.91 59.059 0.00 0.00 0.00 2.941 51 CACACAACACACAGTCAAGA 172 20 0 45.00 57.052 0.00 0.00 0.00 2.948 52 CACAGTCAAGAAGCTACATCCA 163 22 0 45.45 58.995 0.00 0.00 0.00 3.005 53 ACACAGTCAAGAAGCTACATCC 164 22 0 45.45 58.995 0.00 0.00 0.00 3.005 54 ACAACACACAGTCAAGAAGCTAC 169 23 0 43.48 60.052 0.00 0.00 0.00 3.052 55 CACACACACACAACATACACTCT 242 23 0 43.48 60.052 0.00 0.00 0.00 3.052 56 CACAACACACAGTCAAGAAGCTA 170 23 0 43.48 60.052 0.00 0.00 0.00 3.052 57 CAACACACAGTCAAGAAGCTACA 168 23 0 43.48 60.052 0.00 0.00 0.00 3.052 58 GCTACATCCATACACAACACACA 151 23 0 43.48 59.926 0.00 0.00 0.00 3.074 59 CACACAACACACAGTCAAGAAG 172 22 0 45.45 58.896 0.00 0.00 0.00 3.104 60 ACACACACACACAACATACAC 243 21 0 42.86 57.878 0.00 0.00 0.00 3.122 61 CACACAACACACAGTCAAGAA 172 21 0 42.86 57.806 0.00 0.00 0.00 3.194 62 ACACACACACAACATACACTCT 241 22 0 40.91 58.793 0.00 0.00 0.00 3.207 63 ACAACACACAGTCAAGAAGCTA 169 22 0 40.91 58.793 0.00 0.00 0.00 3.207 64 AACACACAGTCAAGAAGCTACA 167 22 0 40.91 58.793 0.00 0.00 0.00 3.207 65 CACACAGTCAAGAAGCTACATCC 165 23 0 47.83 60.238 0.00 0.00 0.00 3.238 66 CACACACACACAACATACACTC 242 22 0 45.45 58.706 0.00 0.00 0.00 3.294 67 ACACACACACACAACATACACTC 243 23 0 43.48 60.300 0.00 0.00 0.00 3.300 68 ATACACAACAGACACACACACAC 190 23 0 43.48 60.300 0.00 0.00 0.00 3.300 69 CAGACACACACACACAACACAC 182 22 0 50.00 61.316 0.00 0.00 0.00 3.316 70 GACACACACACACAACACACAG 180 22 0 50.00 61.316 0.00 0.00 0.00 3.316 71 CACACACACACAACACACAGTC 178 22 0 50.00 61.316 0.00 0.00 0.00 3.316 72 AGCTACATCCATACACAACACAC 152 23 0 43.48 59.675 0.00 0.00 0.00 3.325 73 ACACACAGTCAAGAAGCTACATC 166 23 0 43.48 59.615 0.00 0.00 0.00 3.385 74 CACACACACACAACATACACT 242 21 0 42.86 57.600 0.00 0.00 0.00 3.400 75 ACAGTCAAGAAGCTACATCCA 162 21 0 42.86 57.597 0.00 0.00 0.00 3.403 76 ACACACAGTCAAGAAGCTACAT 166 22 0 40.91 58.590 0.00 0.00 0.00 3.410 77 TGCACAATACCACACATGTTT 122 21 0 38.10 57.554 0.00 0.00 0.00 3.446 78 ACATCCATACACAACACACATGT 148 23 0 39.13 59.542 0.00 0.00 42.88 3.458 79 ACACAACACACAGTCAAGAAG 171 21 0 42.86 57.527 0.00 0.00 0.00 3.473 80 ACACACAACACACAGTCAAGAAG 173 23 0 43.48 60.486 0.00 0.00 0.00 3.486 81 CAACACACAGTCAAGAAGCTAC 168 22 0 45.45 58.445 0.00 0.00 0.00 3.555 82 CACACACACACAACACACA 178 19 0 47.37 57.435 0.00 0.00 0.00 3.565 83 ACACACACACACAACACAC 179 19 0 47.37 57.434 0.00 0.00 0.00 3.566 84 CACAGTCAAGAAGCTACATCCAT 163 23 0 43.48 59.421 0.00 0.00 0.00 3.579 85 AGCTACATCCATACACAACACA 152 22 0 40.91 58.384 0.00 0.00 0.00 3.616 86 ACACAGTCAAGAAGCTACATCCA 164 23 0 43.48 60.620 0.00 0.00 0.00 3.620 87 ATGCACAATACCACACATGTT 123 21 0 38.10 57.337 0.00 0.00 0.00 3.663 88 ACACACACACAACATACACTC 241 21 0 42.86 57.327 0.00 0.00 0.00 3.673 89 ATACACAACAGACACACACAC 190 21 0 42.86 57.327 0.00 0.00 0.00 3.673 90 GCTACATCCATACACAACACAC 151 22 0 45.45 58.308 0.00 0.00 0.00 3.692 91 CACACACAACACACAGTCAAGAA 174 23 0 43.48 60.732 0.00 0.00 0.00 3.732 92 CACAGTCAAGAAGCTACATCC 163 21 0 47.62 57.263 0.00 0.00 0.00 3.737 93 ACACAACAGACACACACACACA 188 22 0 45.45 61.737 0.00 0.00 0.00 3.737 94 ACAGACACACACACACAACACA 183 22 0 45.45 61.737 0.00 0.00 0.00 3.737 95 AGACACACACACACAACACACA 181 22 0 45.45 61.737 0.00 0.00 0.00 3.737 96 ACACACACACAACACACAGTCA 177 22 0 45.45 61.737 0.00 0.00 0.00 3.737 97 AACACACAGTCAAGAAGCTACAT 167 23 0 39.13 59.228 0.00 0.00 0.00 3.772 98 TACACACACACACAACATACACT 244 23 0 39.13 59.170 0.00 0.00 0.00 3.830 99 GACACACACACACAACACA 180 19 0 47.37 57.137 0.00 0.00 0.00 3.863 100 ACACACACACAACACACAG 177 19 0 47.37 57.128 0.00 0.00 0.00 3.872 101 CACACACACAACACACAGT 176 19 0 47.37 57.128 0.00 0.00 0.00 3.872 102 ACAGTCAAGAAGCTACATCCAT 162 22 0 40.91 58.107 0.00 0.00 0.00 3.893 103 ATGCACAATACCACACATGTTT 123 22 0 36.36 58.061 0.00 0.00 0.00 3.939 104 GATCTACACACACACACAACA 248 21 0 42.86 57.055 0.00 0.00 0.00 3.945 105 CACACACACAACATACACTCT 240 21 0 42.86 57.047 0.00 0.00 0.00 3.953 106 CAACACACAGTCAAGAAGCTA 168 21 0 42.86 57.047 0.00 0.00 0.00 3.953 107 AACACACAGTCAAGAAGCTAC 167 21 0 42.86 57.045 0.00 0.00 0.00 3.955 108 AAGCTACATCCATACACAACACA 153 23 0 39.13 59.034 0.00 0.00 0.00 3.966 109 ACACACACACACAACACACAGT 179 22 0 45.45 61.997 0.00 0.00 0.00 3.997 110 GAAGCTACATCCATACACAACACA 154 24 0 41.67 59.997 0.00 0.00 0.00 4.003 111 CACACAGTCAAGAAGCTACATC 165 22 0 45.45 57.988 0.00 0.00 0.00 4.012 112 TCTACACACACACACAACATACA 246 23 0 39.13 58.919 0.00 0.00 0.00 4.081 113 TATACACAACAGACACACACACA 191 23 0 39.13 58.919 0.00 0.00 0.00 4.081 114 ACATCCATACACAACACACATG 148 22 0 40.91 57.887 0.00 0.00 0.00 4.113 115 CATCCATACACAACACACATGT 147 22 0 40.91 57.887 0.00 0.00 42.88 4.113 116 CTACACACACACACAACATACACT 245 24 0 41.67 60.118 0.00 0.00 0.00 4.118 117 TCTACACACACACACAACATACAC 246 24 0 41.67 60.118 0.00 0.00 0.00 4.118 118 TACACACACACACAACATACACTC 244 24 0 41.67 60.118 0.00 0.00 0.00 4.118 119 TATACACAACAGACACACACACAC 191 24 0 41.67 60.118 0.00 0.00 0.00 4.118 120 CACACACACACAACATACACTCTA 242 24 0 41.67 59.879 0.00 0.00 0.00 4.121 121 CTACACACACACACAACATACAC 245 23 0 43.48 58.829 0.00 0.00 0.00 4.171 122 ACACAACACACATGTATATGCACA 140 24 0 37.50 60.177 0.00 0.00 39.26 4.177 123 AACACACAGTCAAGAAGCTACATC 167 24 0 41.67 60.177 0.00 0.00 0.00 4.177 124 TACACACACACACAACATACAC 244 22 0 40.91 57.783 0.00 0.00 0.00 4.217 125 AAGCTACATCCATACACAACACAC 153 24 0 41.67 60.237 0.00 0.00 0.00 4.237 126 AGAAGCTACATCCATACACAACAC 155 24 0 41.67 59.756 0.00 0.00 0.00 4.244 127 GCTACATCCATACACAACACACAT 151 24 0 41.67 60.297 0.00 0.00 0.00 4.297 128 ACACACACACAACATACACTCTA 241 23 0 39.13 58.662 0.00 0.00 0.00 4.338 129 ACACACACACAACATACACTCTAG 241 24 0 41.67 59.639 0.00 0.00 0.00 4.361 130 CACAACACACATGTATATGCACA 139 23 0 39.13 58.624 0.00 0.00 39.26 4.376 131 ACACAACACACATGTATATGCAC 140 23 0 39.13 58.623 0.00 0.00 39.26 4.377 132 CAACACACAGTCAAGAAGCTACAT 168 24 0 41.67 60.416 0.00 0.00 0.00 4.416 133 GATCTACACACACACACAACAT 248 22 0 40.91 57.574 0.00 0.00 0.00 4.426 134 AGAAGCTACATCCATACACAACA 155 23 0 39.13 58.521 0.00 0.00 0.00 4.479 135 CTACACACACACACAACATACA 245 22 0 40.91 57.517 0.00 0.00 0.00 4.483 136 GTCAAGAAGCTACATCCATACACA 159 24 0 41.67 59.515 0.00 0.00 0.00 4.485 137 TACACAACAGACACACACACACA 189 23 0 43.48 61.487 0.00 0.00 0.00 4.487 138 TGCACAATACCACACATGTTTA 122 22 0 36.36 57.471 0.00 0.00 0.00 4.529 139 GAAGCTACATCCATACACAACAC 154 23 0 43.48 58.444 0.00 0.00 0.00 4.556 140 CACACACACAACACACAGTCAAG 176 23 0 47.83 61.577 0.00 0.00 0.00 4.577 141 TGCACAATACCACACATGTTTAAC 122 24 0 37.50 59.401 0.00 0.00 0.00 4.599 142 GCACAATACCACACATGTTTAACA 121 24 0 37.50 59.401 0.00 0.00 0.00 4.599 143 CACACACACAACATACACTCTAGA 240 24 0 41.67 59.399 0.00 0.00 0.00 4.601 144 CACACACAACATACACTCTAGACA 238 24 0 41.67 59.399 0.00 0.00 0.00 4.601 145 CACACAACATACACTCTAGACACA 236 24 0 41.67 59.399 0.00 0.00 0.00 4.601 146 ACACACACAACATACACTCTAGAC 239 24 0 41.67 59.399 0.00 0.00 0.00 4.601 147 ACACACAACATACACTCTAGACAC 237 24 0 41.67 59.399 0.00 0.00 0.00 4.601 148 TACATCCATACACAACACACATGT 149 24 0 37.50 59.387 0.00 0.00 42.88 4.613 149 ACATCCATACACAACACACATGTA 148 24 0 37.50 59.387 0.00 0.00 39.26 4.613 150 CACACAACACACAGTCAAGAAGC 172 23 0 47.83 61.639 0.00 0.00 0.00 4.639 151 AAGCTACATCCATACACAACAC 153 22 0 40.91 57.359 0.00 0.00 0.00 4.641 152 ATCTACACACACACACAACATACA 247 24 0 37.50 59.331 0.00 0.00 0.00 4.669 153 ATATACACAACAGACACACACACA 192 24 0 37.50 59.331 0.00 0.00 0.00 4.669 154 ACACAACACACATGTATATGCA 140 22 0 36.36 57.281 0.00 0.00 39.26 4.719 155 ACAACACACATGTATATGCACA 138 22 0 36.36 57.281 0.00 0.00 39.26 4.719 156 CACAGTCAAGAAGCTACATCCATA 163 24 0 41.67 59.271 0.00 0.00 0.00 4.729 157 CAGTCAAGAAGCTACATCCATACA 161 24 0 41.67 59.271 0.00 0.00 0.00 4.729 158 ACAGTCAAGAAGCTACATCCATAC 162 24 0 41.67 59.271 0.00 0.00 0.00 4.729 159 AGTCAAGAAGCTACATCCATACAC 160 24 0 41.67 59.271 0.00 0.00 0.00 4.729 160 TCAAGAAGCTACATCCATACACA 158 23 0 39.13 58.265 0.00 0.00 0.00 4.735 161 TATGCACAATACCACACATGTT 124 22 0 36.36 57.263 0.00 0.00 0.00 4.737 162 ACACACACAACACACAGTCAAGA 175 23 0 43.48 61.740 0.00 0.00 0.00 4.740 163 TCTACACACACACACAACATAC 246 22 0 40.91 57.255 0.00 0.00 0.00 4.745 164 TATACACAACAGACACACACAC 191 22 0 40.91 57.255 0.00 0.00 0.00 4.745 165 CACAACACACATGTATATGCACAA 139 24 0 37.50 59.225 0.00 0.00 39.26 4.775 166 ACACAACACACAGTCAAGAAGCT 171 23 0 43.48 61.808 0.00 0.00 0.00 4.808 167 CACAACAGACACACACACACAAC 187 23 0 47.83 61.817 0.00 0.00 0.00 4.817 168 CAACAGACACACACACACAACAC 185 23 0 47.83 61.817 0.00 0.00 0.00 4.817 169 TGCACAATACCACACATGTTTAA 122 23 0 34.78 58.157 0.00 0.00 0.00 4.843 170 ACACAACATACACTCTAGACACAG 235 24 0 41.67 59.157 0.00 0.00 0.00 4.843 171 ACACACACAACATACACTCTAGA 239 23 0 39.13 58.151 0.00 0.00 0.00 4.849 172 ACACACAACATACACTCTAGACA 237 23 0 39.13 58.151 0.00 0.00 0.00 4.849 173 ACACAACATACACTCTAGACACA 235 23 0 39.13 58.151 0.00 0.00 0.00 4.849 174 AAGAAGCTACATCCATACACAACA 156 24 0 37.50 59.140 0.00 0.00 0.00 4.860 175 GAAGCTACATCCATACACAACA 154 22 0 40.91 57.096 0.00 0.00 0.00 4.904 176 CACACACACAACATACACTCTAG 240 23 0 43.48 58.081 0.00 0.00 0.00 4.919 177 ATATGCACAATACCACACATGT 125 22 0 36.36 57.054 0.00 0.00 0.00 4.946 178 CTACATCCATACACAACACACA 150 22 0 40.91 57.033 0.00 0.00 0.00 4.967 179 ACACAGTCAAGAAGCTACATCCAT 164 24 0 41.67 60.971 0.00 0.00 0.00 4.971 180 ACAGTCAAGAAGCTACATCCATA 162 23 0 39.13 58.004 0.00 0.00 0.00 4.996 181 AGTCAAGAAGCTACATCCATACA 160 23 0 39.13 58.004 0.00 0.00 0.00 4.996 182 TACACAACACACATGTATATGCACA 141 25 0 36.00 60.005 0.00 0.00 39.26 5.005 183 ACAACACACATGTATATGCACAA 138 23 0 34.78 57.973 0.00 0.00 39.26 5.027 184 TATGCACAATACCACACATGTTT 124 23 0 34.78 57.961 0.00 0.00 0.00 5.039 185 ATGCACAATACCACACATGTTTA 123 23 0 34.78 57.961 0.00 0.00 0.00 5.039 186 GTCAAGAAGCTACATCCATACAC 159 23 0 43.48 57.941 0.00 0.00 0.00 5.059 187 GTCAAGAAGCTACATCCATACACAA 159 25 0 40.00 60.063 0.00 0.00 0.00 5.063 188 TCAAGAAGCTACATCCATACACAAC 158 25 0 40.00 60.063 0.00 0.00 0.00 5.063 189 TCAAGAAGCTACATCCATACACAA 158 24 0 37.50 58.896 0.00 0.00 0.00 5.104 190 GCACAATACCACACATGTTTAAC 121 23 0 39.13 57.839 0.00 0.00 0.00 5.161 191 CACACACAACATACACTCTAGAC 238 23 0 43.48 57.832 0.00 0.00 0.00 5.168 192 CACACAACATACACTCTAGACAC 236 23 0 43.48 57.832 0.00 0.00 0.00 5.168 193 ACACACATGTATATGCACAATACCA 135 25 0 36.00 59.828 15.76 7.60 38.02 5.172 194 ACACATGTATATGCACAATACCACA 133 25 0 36.00 59.828 15.76 4.59 38.43 5.172 195 ACATGTATATGCACAATACCACACA 131 25 0 36.00 59.828 15.76 4.01 38.43 5.172 196 TGTATATGCACAATACCACACATGT 128 25 0 36.00 59.828 15.76 0.00 0.00 5.172 197 CACAACACACAGTCAAGAAGCTAC 170 24 0 45.83 61.177 0.00 0.00 0.00 5.177 198 CTACATCCATACACAACACACATG 150 24 0 41.67 58.810 0.00 0.00 0.00 5.190 199 CAAGAAGCTACATCCATACACAAC 157 24 0 41.67 58.809 0.00 0.00 0.00 5.191 200 TACATCCATACACAACACACATG 149 23 0 39.13 57.795 0.00 0.00 0.00 5.205 201 CATCCATACACAACACACATGTA 147 23 0 39.13 57.795 0.00 0.00 39.26 5.205 202 AGCTACATCCATACACAACACACA 152 24 0 41.67 61.209 0.00 0.00 0.00 5.209 203 ATGCACAATACCACACATGTTTAAC 123 25 0 36.00 59.776 0.00 0.00 0.00 5.224 204 ACATCCATACACAACACACATGTAT 148 25 0 36.00 59.769 0.00 0.00 39.26 5.231 205 ACACAACAGACACACACACACAA 188 23 0 43.48 62.233 0.00 0.00 0.00 5.233 206 ACAACAGACACACACACACAACA 186 23 0 43.48 62.233 0.00 0.00 0.00 5.233 207 AACAGACACACACACACAACACA 184 23 0 43.48 62.233 0.00 0.00 0.00 5.233 208 ACACACACACAACACACAGTCAA 177 23 0 43.48 62.233 0.00 0.00 0.00 5.233 209 GATCTACACACACACACAACATACA 248 25 0 40.00 60.236 0.00 0.00 0.00 5.236 210 ATATGCACAATACCACACATGTT 125 23 0 34.78 57.763 0.00 0.00 0.00 5.237 211 GATCTACACACACACACAACATAC 248 24 0 41.67 58.761 0.00 0.00 0.00 5.239 212 ATCTACACACACACACAACATAC 247 23 0 39.13 57.745 0.00 0.00 0.00 5.255 213 ATATACACAACAGACACACACAC 192 23 0 39.13 57.745 0.00 0.00 0.00 5.255 214 GCACAATACCACACATGTTTAACAG 121 25 0 40.00 60.292 0.00 0.00 0.00 5.292 215 CACACAACATACACTCTAGACACAG 236 25 0 44.00 60.293 0.00 0.00 0.00 5.293 216 CAAGAAGCTACATCCATACACAACA 157 25 0 40.00 60.294 0.00 0.00 0.00 5.294 217 CTACATCCATACACAACACACATGT 150 25 0 40.00 60.294 0.00 0.00 42.88 5.294 218 AAGAAGCTACATCCATACACAACAC 156 25 0 40.00 60.294 0.00 0.00 0.00 5.294 219 CAGTCAAGAAGCTACATCCATAC 161 23 0 43.48 57.686 0.00 0.00 0.00 5.314 220 CACAACACACATGTATATGCACAAT 139 25 0 36.00 59.605 0.00 0.00 39.26 5.395 221 ATGCACAATACCACACATGTTTAA 123 24 0 33.33 58.600 0.00 0.00 0.00 5.400 222 CACAGTCAAGAAGCTACATCCATAC 163 25 0 44.00 60.408 0.00 0.00 0.00 5.408 223 CAGTCAAGAAGCTACATCCATACAC 161 25 0 44.00 60.408 0.00 0.00 0.00 5.408 224 CACAACATACACTCTAGACACAG 234 23 0 43.48 57.579 0.00 0.00 0.00 5.421 225 CAAGAAGCTACATCCATACACAA 157 23 0 39.13 57.536 0.00 0.00 0.00 5.464 226 CTACATCCATACACAACACACAT 150 23 0 39.13 57.536 0.00 0.00 0.00 5.464 227 AAGAAGCTACATCCATACACAAC 156 23 0 39.13 57.535 0.00 0.00 0.00 5.465 228 ATCTACACACACACACAACATACAC 247 25 0 40.00 60.466 0.00 0.00 0.00 5.466 229 ATATACACAACAGACACACACACAC 192 25 0 40.00 60.466 0.00 0.00 0.00 5.466 230 TACACAACACACATGTATATGCAC 141 24 0 37.50 58.507 0.00 0.00 39.26 5.493 231 GATCTACACACACACACAACATA 248 23 0 39.13 57.495 0.00 0.00 0.00 5.505 232 CCATACACAACACACATGTATATGC 144 25 0 40.00 59.494 2.84 0.00 39.26 5.506 233 CACACATGTATATGCACAATACCAC 134 25 0 40.00 59.494 15.76 0.00 38.43 5.506 234 CACATGTATATGCACAATACCACAC 132 25 0 40.00 59.494 15.76 0.00 38.43 5.506 235 CACACACACAACATACACTCTAGAC 240 25 0 44.00 60.520 0.00 0.00 0.00 5.520 236 CACACACAACATACACTCTAGACAC 238 25 0 44.00 60.520 0.00 0.00 0.00 5.520 237 ACACACACACACAACATACACTCT 243 24 0 41.67 61.564 0.00 0.00 0.00 5.564 238 ACACAACACACAGTCAAGAAGCTA 171 24 0 41.67 61.564 0.00 0.00 0.00 5.564 239 ACAACACACAGTCAAGAAGCTACA 169 24 0 41.67 61.564 0.00 0.00 0.00 5.564 240 ACAACACACATGTATATGCACAAT 138 24 0 33.33 58.422 0.00 0.00 39.26 5.578 241 AGACACACACACACAACACACAG 181 23 0 47.83 62.582 0.00 0.00 0.00 5.582 242 CACACACAACACACAGTCAAGAAG 174 24 0 45.83 61.586 0.00 0.00 0.00 5.586 243 ATATGCACAATACCACACATGTTT 125 24 0 33.33 58.412 0.00 0.00 0.00 5.588 244 CACACATGTATATGCACAATACCA 134 24 0 37.50 58.313 15.76 7.60 38.43 5.687 245 CACATGTATATGCACAATACCACA 132 24 0 37.50 58.313 15.76 4.59 38.43 5.687 246 CATGTATATGCACAATACCACACA 130 24 0 37.50 58.313 15.76 4.01 31.46 5.687 247 TGTATATGCACAATACCACACATG 128 24 0 37.50 58.313 15.76 0.00 0.00 5.687 248 ACACACATGTATATGCACAATACC 135 24 0 37.50 58.312 15.76 3.34 38.02 5.688 249 ACACATGTATATGCACAATACCAC 133 24 0 37.50 58.312 15.76 0.00 38.43 5.688 250 ACATGTATATGCACAATACCACAC 131 24 0 37.50 58.312 15.76 0.00 38.43 5.688 251 GTATATGCACAATACCACACATGT 127 24 0 37.50 58.312 9.75 0.00 0.00 5.688 252 ACACAACACACATGTATATGCACAA 140 25 0 36.00 60.700 0.00 0.00 39.26 5.700 253 CACACAGTCAAGAAGCTACATCCA 165 24 0 45.83 61.738 0.00 0.00 0.00 5.738 254 ACACACAGTCAAGAAGCTACATCC 166 24 0 45.83 61.739 0.00 0.00 0.00 5.739 255 CACACACACACAACATACACTCTAG 242 25 0 44.00 60.746 0.00 0.00 0.00 5.746 256 CATCCATACACAACACACATGTAT 147 24 0 37.50 58.249 0.00 0.00 39.26 5.751 257 TACATCCATACACAACACACATGTA 149 25 0 36.00 59.243 0.00 0.00 39.26 5.757 258 ACACAGTCAAGAAGCTACATCCATA 164 25 0 40.00 60.765 0.00 0.00 0.00 5.765 259 ACAGTCAAGAAGCTACATCCATACA 162 25 0 40.00 60.765 0.00 0.00 0.00 5.765 260 AGTCAAGAAGCTACATCCATACACA 160 25 0 40.00 60.765 0.00 0.00 0.00 5.765 261 TACACAACACACATGTATATGCA 141 23 0 34.78 57.213 0.00 0.00 39.26 5.787 262 ATACACAACAGACACACACACACA 190 24 0 41.67 61.800 0.00 0.00 0.00 5.800 263 TGTATATACACAACAGACACACACA 195 25 0 36.00 59.190 16.53 0.00 0.00 5.810 264 TATATACACAACAGACACACACACA 193 25 0 36.00 59.190 0.00 0.00 0.00 5.810 265 CAGACACACACACACAACACACA 182 23 0 47.83 62.820 0.00 0.00 0.00 5.820 266 CACACACACACAACACACAGTCA 178 23 0 47.83 62.820 0.00 0.00 0.00 5.820 267 ACAGACACACACACACAACACAC 183 23 0 47.83 62.822 0.00 0.00 0.00 5.822 268 GACACACACACACAACACACAGT 180 23 0 47.83 62.822 0.00 0.00 0.00 5.822 269 ACACACACACACAACACACAGTC 179 23 0 47.83 62.822 0.00 0.00 0.00 5.822 270 TGCACAATACCACACATGTTTAACA 122 25 0 36.00 60.875 0.00 0.00 0.00 5.875 271 ACACACACACAACATACACTCTAGA 241 25 0 40.00 60.877 0.00 0.00 0.00 5.877 272 ACACACACAACATACACTCTAGACA 239 25 0 40.00 60.877 0.00 0.00 0.00 5.877 273 ACACACAACATACACTCTAGACACA 237 25 0 40.00 60.877 0.00 0.00 0.00 5.877 274 CTACACACACACACAACATACACTC 245 25 0 44.00 60.972 0.00 0.00 0.00 5.972 275 TACACAACAGACACACACACACAA 189 24 0 41.67 61.974 0.00 0.00 0.00 5.974 276 CAACACACATGTATATGCACAATACC 137 26 0 38.46 60.015 15.76 3.34 39.26 6.015 277 CATACACAACACACATGTATATGCAC 143 26 0 38.46 60.016 0.00 0.00 39.26 6.016 278 CACAATACCACACATGTTTAACAGA 120 25 0 36.00 58.967 3.60 0.00 0.00 6.033 279 CACAATACCACACATGTTTAACAGAC 120 26 0 38.46 60.070 3.60 0.00 0.00 6.070 280 CATACACAACACACATGTATATGCA 143 25 0 36.00 58.914 0.00 0.00 39.26 6.086 281 ATACACAACACACATGTATATGCAC 142 25 0 36.00 58.914 0.00 0.00 39.26 6.086 282 GAAGCTACATCCATACACAACACAC 154 25 0 44.00 61.090 0.00 0.00 0.00 6.090 283 AACACACATGTATATGCACAATACC 136 25 0 36.00 58.907 15.76 3.34 39.26 6.093 284 GTATATGCACAATACCACACATGTT 127 25 0 36.00 58.907 9.75 0.00 0.00 6.093 285 CTACATCCATACACAACACACATGTA 150 26 0 38.46 60.124 0.00 0.00 39.26 6.124 286 GTATATACACAACAGACACACACAC 194 25 0 40.00 58.872 8.63 0.00 0.00 6.128 287 TATGCACAATACCACACATGTTTA 124 24 0 33.33 57.868 0.00 0.00 0.00 6.132 288 CATGTATATGCACAATACCACACATG 130 26 0 38.46 59.850 12.26 9.17 31.83 6.150 289 ACATGTATATGCACAATACCACACAT 131 26 0 34.62 60.180 15.76 5.82 38.43 6.180 290 ATGTATATGCACAATACCACACATGT 129 26 0 34.62 60.180 15.76 0.00 31.46 6.180 291 ACACACACAACACACAGTCAAGAA 175 24 0 41.67 62.217 0.00 0.00 0.00 6.217 292 AGAAGCTACATCCATACACAACACA 155 25 0 40.00 61.231 0.00 0.00 0.00 6.231 293 CAACACACAGTCAAGAAGCTACATC 168 25 0 44.00 61.257 0.00 0.00 0.00 6.257 294 CATGTATATGCACAATACCACACAT 130 25 0 36.00 58.730 15.76 5.82 31.46 6.270 295 ATGTATATGCACAATACCACACATG 129 25 0 36.00 58.730 15.76 0.00 31.46 6.270 296 TGTATATACACAACAGACACACACAC 195 26 0 38.46 60.291 16.53 0.00 0.00 6.291 297 GTATATACACAACAGACACACACACA 194 26 0 38.46 60.291 8.63 0.00 0.00 6.291 298 TATATACACAACAGACACACACACAC 193 26 0 38.46 60.291 0.00 0.00 0.00 6.291 299 ATACACAACACACATGTATATGCA 142 24 0 33.33 57.692 0.00 0.00 39.26 6.308 300 CACAATACCACACATGTTTAACAG 120 24 0 37.50 57.686 0.00 0.00 0.00 6.314 301 TATATGCACAATACCACACATGTT 126 24 0 33.33 57.678 0.00 0.00 0.00 6.322 302 TGTATATACACAACAGACACACAC 195 24 0 37.50 57.663 16.53 0.00 0.00 6.337 303 GTATATACACAACAGACACACACA 194 24 0 37.50 57.663 8.63 0.00 0.00 6.337 304 TATATACACAACAGACACACACAC 193 24 0 37.50 57.663 0.00 0.00 0.00 6.337 305 TCTACACACACACACAACATACACT 246 25 0 40.00 61.338 0.00 0.00 0.00 6.338 306 TACACACACACACAACATACACTCT 244 25 0 40.00 61.338 0.00 0.00 0.00 6.338 307 ACACACACACACAACATACACTCTA 243 25 0 40.00 61.338 0.00 0.00 0.00 6.338 308 ATACACAACACACATGTATATGCACA 142 26 0 34.62 60.348 0.00 0.00 39.26 6.348 309 AACACACATGTATATGCACAATACCA 136 26 0 34.62 60.349 15.76 7.60 39.26 6.349 310 TGTATATGCACAATACCACACATGTT 128 26 0 34.62 60.349 15.76 0.00 0.00 6.349 311 GCTACATCCATACACAACACACATG 151 25 0 44.00 61.374 0.00 0.00 0.00 6.374 312 TATGCACAATACCACACATGTTTAAC 124 26 0 34.62 59.626 0.00 0.00 0.00 6.374 313 TACATCCATACACAACACACATGTAT 149 26 0 34.62 59.616 0.00 0.00 39.26 6.384 314 ACATCCATACACAACACACATGTATA 148 26 0 34.62 59.616 0.00 0.00 39.26 6.384 315 TATGCACAATACCACACATGTTTAA 124 25 0 32.00 58.487 0.00 0.00 0.00 6.513 316 ATGTATATGCACAATACCACACAT 129 24 0 33.33 57.486 15.76 5.82 31.46 6.514 317 TACACAACACACATGTATATGCACAA 141 26 0 34.62 60.515 0.00 0.00 39.26 6.515 318 AGCTACATCCATACACAACACACAT 152 25 0 40.00 61.523 0.00 0.00 0.00 6.523 319 CACAACACACATGTATATGCACAATA 139 26 0 34.62 59.461 0.00 0.00 39.26 6.539 320 ACAACACACATGTATATGCACAATAC 138 26 0 34.62 59.461 12.84 12.84 39.26 6.539 321 GTATATGCACAATACCACACATGTTT 127 26 0 34.62 59.457 9.75 0.00 0.00 6.543 322 TATACACAACAGACACACACACACA 191 25 0 40.00 61.566 0.00 0.00 0.00 6.566 323 CATACACAACACACATGTATATGC 143 24 0 37.50 57.398 0.00 0.00 39.26 6.602 324 TCCATACACAACACACATGTATATGC 145 26 0 38.46 60.680 2.84 0.00 39.26 6.680 325 ACAACACACATGTATATGCACAATA 138 25 0 32.00 58.316 0.00 0.00 39.26 6.684 326 TATATGCACAATACCACACATGTTT 126 25 0 32.00 58.306 0.00 0.00 0.00 6.694 327 ATATGCACAATACCACACATGTTTA 125 25 0 32.00 58.306 0.00 0.00 0.00 6.694 328 AAGCTACATCCATACACAACACACA 153 25 0 40.00 61.694 0.00 0.00 0.00 6.694 329 CACACACACAACACACAGTCAAGA 176 24 0 45.83 62.783 0.00 0.00 0.00 6.783 330 CACACAACACACAGTCAAGAAGCT 172 24 0 45.83 62.850 0.00 0.00 0.00 6.850 331 CATCCATACACAACACACATGTATA 147 25 0 36.00 58.150 0.00 0.00 39.26 6.850 332 TCCATACACAACACACATGTATATG 145 25 0 36.00 58.150 1.80 1.80 39.26 6.850 333 ACAACACACAGTCAAGAAGCTACAT 169 25 0 40.00 61.863 0.00 0.00 0.00 6.863 334 CCATACACAACACACATGTATATGCA 144 26 0 38.46 60.902 2.84 0.00 39.26 6.902 335 CACACATGTATATGCACAATACCACA 134 26 0 38.46 60.902 15.76 4.59 38.43 6.902 336 CACATGTATATGCACAATACCACACA 132 26 0 38.46 60.902 15.76 4.01 38.43 6.902 337 ACACACATGTATATGCACAATACCAC 135 26 0 38.46 60.902 15.76 0.00 38.02 6.902 338 ACACATGTATATGCACAATACCACAC 133 26 0 38.46 60.902 15.76 0.00 38.43 6.902 339 CAACACACATGTATATGCACAATAC 137 25 0 36.00 58.019 12.84 12.84 39.26 6.981 340 ACACAACACACATGTATATGCACAAT 140 26 0 34.62 61.018 0.00 0.00 39.26 7.018 341 CACACAGTCAAGAAGCTACATCCAT 165 25 0 44.00 62.029 0.00 0.00 0.00 7.029 342 ATATGCACAATACCACACATGTTTAAC 125 27 0 33.33 59.967 0.00 0.00 0.00 7.033 343 ACATCCATACACAACACACATGTATAT 148 27 0 33.33 59.963 0.00 0.00 39.26 7.037 344 ATATGCACAATACCACACATGTTTAA 125 26 0 30.77 58.886 0.00 0.00 0.00 7.114 345 GTCAAGAAGCTACATCCATACACAAC 159 26 0 42.31 61.115 0.00 0.00 0.00 7.115 346 TACACACACACACAACATACACTCTA 244 26 0 38.46 61.130 0.00 0.00 0.00 7.130 347 ATGCACAATACCACACATGTTTAACA 123 26 0 34.62 61.187 0.00 0.00 0.00 7.187 348 AACACACAGTCAAGAAGCTACATCC 167 25 0 44.00 62.198 0.00 0.00 0.00 7.198 349 AGTCAAGAAGCTACATCCATACACAA 160 26 0 38.46 61.252 0.00 0.00 0.00 7.252 350 ATACACAACAGACACACACACACAA 190 25 0 40.00 62.257 0.00 0.00 0.00 7.257 351 GATCTACACACACACACAACATACAC 248 26 0 42.31 61.271 0.00 0.00 0.00 7.271 352 CAAGAAGCTACATCCATACACAACAC 157 26 0 42.31 61.334 0.00 0.00 0.00 7.334 353 CATCCATACACAACACACATGTATATG 147 27 0 37.04 59.649 1.80 1.80 39.26 7.351 354 CATCCATACACAACACACATGTATAT 147 26 0 34.62 58.558 0.00 0.00 39.26 7.442 355 ATCCATACACAACACACATGTATATG 146 26 0 34.62 58.558 1.80 1.80 39.26 7.442 356 CTACATCCATACACAACACACATGTAT 150 27 0 37.04 60.450 0.00 0.00 39.26 7.450 357 GCACAATACCACACATGTTTAACAGA 121 26 0 38.46 61.452 3.60 0.00 0.00 7.452 358 TCAAGAAGCTACATCCATACACAACA 158 26 0 38.46 61.474 0.00 0.00 0.00 7.474 359 CACAACACACATGTATATGCACAATAC 139 27 0 37.04 60.497 12.84 12.84 39.26 7.497 360 GACATAGAACACACGTGTATATACA 210 25 0 36.00 57.481 16.53 14.12 44.80 7.519 361 TACATCCATACACAACACACATGTATA 149 27 0 33.33 59.474 0.00 0.00 39.26 7.526 362 CACACAACACACAGTCAAGAAGCTA 172 25 0 44.00 62.579 0.00 0.00 0.00 7.579 363 CACAACACACAGTCAAGAAGCTACA 170 25 0 44.00 62.579 0.00 0.00 0.00 7.579 364 ACACAACACACAGTCAAGAAGCTAC 171 25 0 44.00 62.581 0.00 0.00 0.00 7.581 365 ATCTACACACACACACAACATACACT 247 26 0 38.46 61.634 0.00 0.00 0.00 7.634 366 ATCCATACACAACACACATGTATAT 146 25 0 32.00 57.351 0.00 0.00 39.26 7.649 367 ATGTATATGCACAATACCACACATGTT 129 27 0 33.33 60.670 15.76 0.00 31.46 7.670 368 TGCACAATACCACACATGTTTAACAG 122 26 0 38.46 61.672 0.00 0.00 0.00 7.672 369 ACACACAACATACACTCTAGACACAG 237 26 0 42.31 61.676 0.00 0.00 0.00 7.676 370 GTATATGCACAATACCACACATGTTTA 127 27 0 33.33 59.323 9.75 0.00 0.00 7.677 371 AAGAAGCTACATCCATACACAACACA 156 26 0 38.46 61.698 0.00 0.00 0.00 7.698 372 TATATGCACAATACCACACATGTTTA 126 26 0 30.77 58.208 0.00 0.00 0.00 7.792 373 CACACAGTCAAGAAGCTACATCCATA 165 26 0 42.31 61.796 0.00 0.00 0.00 7.796 374 CACAGTCAAGAAGCTACATCCATACA 163 26 0 42.31 61.796 0.00 0.00 0.00 7.796 375 CAGTCAAGAAGCTACATCCATACACA 161 26 0 42.31 61.796 0.00 0.00 0.00 7.796 376 ACACAGTCAAGAAGCTACATCCATAC 164 26 0 42.31 61.797 0.00 0.00 0.00 7.797 377 ACAGTCAAGAAGCTACATCCATACAC 162 26 0 42.31 61.797 0.00 0.00 0.00 7.797 378 ATACACAACACACATGTATATGCACAA 142 27 0 33.33 60.828 0.00 0.00 39.26 7.828 379 TACACAACACACATGTATATGCACAAT 141 27 0 33.33 60.828 0.00 0.00 39.26 7.828 380 ACACAACACACATGTATATGCACAATA 140 27 0 33.33 60.828 0.00 0.00 39.26 7.828 381 TGTATATGCACAATACCACACATGTTT 128 27 0 33.33 60.831 15.76 0.00 0.00 7.831 382 ATATACACAACAGACACACACACACA 192 26 0 38.46 61.853 0.00 0.00 0.00 7.853 383 CACACACACACAACATACACTCTAGA 242 26 0 42.31 61.893 0.00 0.00 0.00 7.893 384 CACACACACAACATACACTCTAGACA 240 26 0 42.31 61.893 0.00 0.00 0.00 7.893 385 CACACACAACATACACTCTAGACACA 238 26 0 42.31 61.893 0.00 0.00 0.00 7.893 386 ACACACACACAACATACACTCTAGAC 241 26 0 42.31 61.894 0.00 0.00 0.00 7.894 387 ACACACACAACATACACTCTAGACAC 239 26 0 42.31 61.894 0.00 0.00 0.00 7.894 388 TACACAACAGACACACACACACAAC 189 25 0 44.00 62.964 0.00 0.00 0.00 7.964 389 ACACACACAACACACAGTCAAGAAG 175 25 0 44.00 62.976 0.00 0.00 0.00 7.976 390 AAGCTACATCCATACACAACACACAT 153 26 0 38.46 61.979 0.00 0.00 0.00 7.979 391 ATCCATACACAACACACATGTATATGC 146 27 0 37.04 60.986 2.84 0.00 39.26 7.986 392 TATGCACAATACCACACATGTTTAACA 124 27 0 33.33 60.991 0.00 0.00 0.00 7.991 393 TATACACAACAGACACACACACACAA 191 26 0 38.46 62.015 0.00 0.00 0.00 8.015 394 TCTACACACACACACAACATACACTC 246 26 0 42.31 62.110 0.00 0.00 0.00 8.110 395 CTACACACACACACAACATACACTCT 245 26 0 42.31 62.114 0.00 0.00 0.00 8.114 396 ACACACACACACAACATACACTCTAG 243 26 0 42.31 62.114 0.00 0.00 0.00 8.114 397 CACATGTATATGCACAATACCACACAT 132 27 0 37.04 61.200 15.76 5.82 38.43 8.200 398 ACATGTATATGCACAATACCACACATG 131 27 0 37.04 61.200 16.36 16.36 38.43 8.200 399 CATGTATATGCACAATACCACACATGT 130 27 0 37.04 61.200 14.06 0.00 33.01 8.200 400 TATATGCACAATACCACACATGTTTAA 126 27 0 29.63 58.771 0.00 0.00 0.00 8.229 401 AGAAGCTACATCCATACACAACACAC 155 26 0 42.31 62.236 0.00 0.00 0.00 8.236 402 GTATATACACAACAGACACACACACAC 194 27 0 40.74 61.287 8.63 0.00 0.00 8.287 403 CATACACAACACACATGTATATGCACA 143 27 0 37.04 61.352 0.00 0.00 39.26 8.352 404 CAACACACATGTATATGCACAATACCA 137 27 0 37.04 61.358 15.76 7.60 39.26 8.358 405 ACAACACACATGTATATGCACAATACC 138 27 0 37.04 61.358 15.76 3.34 39.26 8.358 406 AACACACATGTATATGCACAATACCAC 136 27 0 37.04 61.358 15.76 0.00 39.26 8.358 407 CACAATACCACACATGTTTAACAGACA 120 27 0 37.04 61.410 3.60 0.00 0.00 8.410 408 GAAGCTACATCCATACACAACACACA 154 26 0 42.31 62.452 0.00 0.00 0.00 8.452 409 AGCTACATCCATACACAACACACATG 152 26 0 42.31 62.512 0.00 0.00 0.00 8.512 410 ACAACACACAGTCAAGAAGCTACATC 169 26 0 42.31 62.610 0.00 0.00 0.00 8.610 411 TGTATATACACAACAGACACACACACA 195 27 0 37.04 61.634 16.53 0.00 0.00 8.634 412 TATATACACAACAGACACACACACACA 193 27 0 37.04 61.634 0.00 0.00 0.00 8.634 413 GCTACATCCATACACAACACACATGT 151 26 0 42.31 62.728 0.00 0.00 42.88 8.728 414 CACAACACACAGTCAAGAAGCTACAT 170 26 0 42.31 62.828 0.00 0.00 0.00 8.828 415 CCATACACAACACACATGTATATGCAC 144 27 0 40.74 61.873 2.84 0.00 39.26 8.873 416 CACACATGTATATGCACAATACCACAC 134 27 0 40.74 61.873 15.76 0.00 38.43 8.873 417 CTACACACACACACAACATACACTCTA 245 27 0 40.74 61.887 0.00 0.00 0.00 8.887 418 TACACACACACACAACATACACTCTAG 244 27 0 40.74 61.887 0.00 0.00 0.00 8.887 419 ATGCACAATACCACACATGTTTAACAG 123 27 0 37.04 61.943 0.00 0.00 0.00 8.943 420 TCCATACACAACACACATGTATATGCA 145 27 0 37.04 62.020 2.84 0.00 39.26 9.020 421 CAGTCAAGAAGCTACATCCATACACAA 161 27 0 40.74 62.221 0.00 0.00 0.00 9.221 422 AGTCAAGAAGCTACATCCATACACAAC 160 27 0 40.74 62.222 0.00 0.00 0.00 9.222 423 ACACACATGTATATGCACAATACCACA 135 27 0 37.04 62.236 15.76 4.59 38.02 9.236 424 ACACATGTATATGCACAATACCACACA 133 27 0 37.04 62.236 15.76 4.01 38.43 9.236 425 ATATACACAACAGACACACACACACAA 192 27 0 37.04 62.277 0.00 0.00 0.00 9.277 426 GATCTACACACACACACAACATACACT 248 27 0 40.74 62.366 0.00 0.00 0.00 9.366 427 ATCTACACACACACACAACATACACTC 247 27 0 40.74 62.366 0.00 0.00 0.00 9.366 428 GCACAATACCACACATGTTTAACAGAC 121 27 0 40.74 62.394 3.60 0.00 0.00 9.394 429 GTCAAGAAGCTACATCCATACACAACA 159 27 0 40.74 62.430 0.00 0.00 0.00 9.430 430 TCAAGAAGCTACATCCATACACAACAC 158 27 0 40.74 62.430 0.00 0.00 0.00 9.430 431 GCTACATCCATACACAACACACATGTA 151 27 0 40.74 62.480 0.00 0.00 39.26 9.480 432 CACACACAACATACACTCTAGACACAG 238 27 0 44.44 62.609 0.00 0.00 0.00 9.609 433 CAAGAAGCTACATCCATACACAACACA 157 27 0 40.74 62.643 0.00 0.00 0.00 9.643 434 AAGAAGCTACATCCATACACAACACAC 156 27 0 40.74 62.644 0.00 0.00 0.00 9.644 435 GAAGCTACATCCATACACAACACACAT 154 27 0 40.74 62.698 0.00 0.00 0.00 9.698 436 CACACAGTCAAGAAGCTACATCCATAC 165 27 0 44.44 62.728 0.00 0.00 0.00 9.728 437 CACAGTCAAGAAGCTACATCCATACAC 163 27 0 44.44 62.728 0.00 0.00 0.00 9.728 438 TGCACAATACCACACATGTTTAACAGA 122 27 0 37.04 62.765 3.60 0.00 0.00 9.765 439 CACACACACACAACATACACTCTAGAC 242 27 0 44.44 62.814 0.00 0.00 0.00 9.814 440 CACACACACAACATACACTCTAGACAC 240 27 0 44.44 62.814 0.00 0.00 0.00 9.814 441 AAGCTACATCCATACACAACACACATG 153 27 0 40.74 62.910 0.00 0.00 0.00 9.910 442 TATACACAACAGACACACACACACAAC 191 27 0 40.74 62.936 0.00 0.00 0.00 9.936 primer3-2.3.6/test/primer1_th_list_last/SP01-21.txt.rev0000664000077300007730000011235712231156243021433 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 ACACACACACACAACACACA 159 20 0 45.00 59.314 0.00 0.00 0.00 0.686 1 ACACACACACAACACACAGT 157 20 0 45.00 59.025 0.00 0.00 0.00 0.975 2 ACACAACAGACACACACACAC 168 21 0 47.62 60.105 0.00 0.00 0.00 1.105 3 ACAGACACACACACACAACAC 163 21 0 47.62 60.105 0.00 0.00 0.00 1.105 4 AGACACACACACACAACACAC 161 21 0 47.62 60.105 0.00 0.00 0.00 1.105 5 ACACACACACAACACACAGTC 157 21 0 47.62 60.105 0.00 0.00 0.00 1.105 6 CACAACAGACACACACACACA 167 21 0 47.62 60.105 0.00 0.00 0.00 1.105 7 CAGACACACACACACAACACA 162 21 0 47.62 60.105 0.00 0.00 0.00 1.105 8 CACACACACAACACACAGTCA 156 21 0 47.62 60.105 0.00 0.00 0.00 1.105 9 ACACAACAGACACACACACA 168 20 0 45.00 58.738 0.00 0.00 0.00 1.262 10 ACAACAGACACACACACACA 166 20 0 45.00 58.738 0.00 0.00 0.00 1.262 11 ACAGACACACACACACAACA 163 20 0 45.00 58.738 0.00 0.00 0.00 1.262 12 AGACACACACACACAACACA 161 20 0 45.00 58.738 0.00 0.00 0.00 1.262 13 ACACACACAACACACAGTCA 155 20 0 45.00 58.738 0.00 0.00 0.00 1.262 14 GACACACACACACAACACAC 160 20 0 50.00 58.645 0.00 0.00 0.00 1.355 15 CACACACACACAACACACAG 158 20 0 50.00 58.642 0.00 0.00 0.00 1.358 16 GACACACACACACAACACACA 160 21 0 47.62 60.374 0.00 0.00 0.00 1.374 17 ACACACACACACAACACACAG 159 21 0 47.62 60.374 0.00 0.00 0.00 1.374 18 CACACACACACAACACACAGT 158 21 0 47.62 60.374 0.00 0.00 0.00 1.374 19 ACAACAGACACACACACACAA 166 21 0 42.86 59.421 0.00 0.00 0.00 1.579 20 AACAGACACACACACACAACA 164 21 0 42.86 59.421 0.00 0.00 0.00 1.579 21 ACACACACAACACACAGTCAA 155 21 0 42.86 59.421 0.00 0.00 0.00 1.579 22 CACAACAGACACACACACAC 167 20 0 50.00 58.362 0.00 0.00 0.00 1.638 23 CAGACACACACACACAACAC 162 20 0 50.00 58.362 0.00 0.00 0.00 1.638 24 CACACACACAACACACAGTC 156 20 0 50.00 58.362 0.00 0.00 0.00 1.638 25 CAACAGACACACACACACAAC 165 21 0 47.62 59.044 0.00 0.00 0.00 1.956 26 ACAACACACAGTCAAGAAGCT 149 21 0 42.86 58.935 0.00 0.00 0.00 2.065 27 TACACAACAGACACACACACAC 169 22 0 45.45 59.916 0.00 0.00 0.00 2.084 28 ACACACAACACACAGTCAAGA 153 21 0 42.86 58.870 0.00 0.00 0.00 2.130 29 CACAACACACAGTCAAGAAGC 150 21 0 47.62 58.840 0.00 0.00 0.00 2.160 30 CACACACAACACACAGTCAAGA 154 22 0 45.45 60.174 0.00 0.00 0.00 2.174 31 CACACACAACACACAGTCAAG 154 21 0 47.62 58.774 0.00 0.00 0.00 2.226 32 CACAACACACAGTCAAGAAGCT 150 22 0 45.45 60.239 0.00 0.00 0.00 2.239 33 CAACAGACACACACACACAA 165 20 0 45.00 57.629 0.00 0.00 0.00 2.371 34 CACACACAACACACAGTCAA 154 20 0 45.00 57.629 0.00 0.00 0.00 2.371 35 AACAGACACACACACACAAC 164 20 0 45.00 57.627 0.00 0.00 0.00 2.373 36 TACACAACAGACACACACACA 169 21 0 42.86 58.599 0.00 0.00 0.00 2.401 37 ACACACACAACACACAGTCAAG 155 22 0 45.45 60.432 0.00 0.00 0.00 2.432 38 ACACACAACACACAGTCAAGAA 153 22 0 40.91 59.519 0.00 0.00 0.00 2.481 39 ACACAACACACAGTCAAGAAGC 151 22 0 45.45 60.497 0.00 0.00 0.00 2.497 40 ACAACACACAGTCAAGAAGC 149 20 0 45.00 57.405 0.00 0.00 0.00 2.595 41 ACACACAACACACAGTCAAG 153 20 0 45.00 57.337 0.00 0.00 0.00 2.663 42 ACACACACACACAACATACACT 223 22 0 40.91 59.322 0.00 0.00 0.00 2.678 43 CACAACAGACACACACACACAA 167 22 0 45.45 60.688 0.00 0.00 0.00 2.688 44 CAACAGACACACACACACAACA 165 22 0 45.45 60.688 0.00 0.00 0.00 2.688 45 CACACACACAACACACAGTCAA 156 22 0 45.45 60.688 0.00 0.00 0.00 2.688 46 ACAACAGACACACACACACAAC 166 22 0 45.45 60.689 0.00 0.00 0.00 2.689 47 AACAGACACACACACACAACAC 164 22 0 45.45 60.689 0.00 0.00 0.00 2.689 48 CAACACACAGTCAAGAAGCT 148 20 0 45.00 57.112 0.00 0.00 0.00 2.888 49 ACACACAGTCAAGAAGCTACA 146 21 0 42.86 58.106 0.00 0.00 0.00 2.894 50 ATACACAACAGACACACACACA 170 22 0 40.91 59.059 0.00 0.00 0.00 2.941 51 CACACAACACACAGTCAAGA 152 20 0 45.00 57.052 0.00 0.00 0.00 2.948 52 CACAGTCAAGAAGCTACATCCA 143 22 0 45.45 58.995 0.00 0.00 0.00 3.005 53 ACACAGTCAAGAAGCTACATCC 144 22 0 45.45 58.995 0.00 0.00 0.00 3.005 54 ACAACACACAGTCAAGAAGCTAC 149 23 0 43.48 60.052 0.00 0.00 0.00 3.052 55 CACACACACACAACATACACTCT 222 23 0 43.48 60.052 0.00 0.00 0.00 3.052 56 CACAACACACAGTCAAGAAGCTA 150 23 0 43.48 60.052 0.00 0.00 0.00 3.052 57 CAACACACAGTCAAGAAGCTACA 148 23 0 43.48 60.052 0.00 0.00 0.00 3.052 58 GCTACATCCATACACAACACACA 131 23 0 43.48 59.926 0.00 0.00 0.00 3.074 59 CACACAACACACAGTCAAGAAG 152 22 0 45.45 58.896 0.00 0.00 0.00 3.104 60 ACACACACACACAACATACAC 223 21 0 42.86 57.878 0.00 0.00 0.00 3.122 61 CACACAACACACAGTCAAGAA 152 21 0 42.86 57.806 0.00 0.00 0.00 3.194 62 ACACACACACAACATACACTCT 221 22 0 40.91 58.793 0.00 0.00 0.00 3.207 63 ACAACACACAGTCAAGAAGCTA 149 22 0 40.91 58.793 0.00 0.00 0.00 3.207 64 AACACACAGTCAAGAAGCTACA 147 22 0 40.91 58.793 0.00 0.00 0.00 3.207 65 CACACAGTCAAGAAGCTACATCC 145 23 0 47.83 60.238 0.00 0.00 0.00 3.238 66 CACACACACACAACATACACTC 222 22 0 45.45 58.706 0.00 0.00 0.00 3.294 67 ACACACACACACAACATACACTC 223 23 0 43.48 60.300 0.00 0.00 0.00 3.300 68 ATACACAACAGACACACACACAC 170 23 0 43.48 60.300 0.00 0.00 0.00 3.300 69 CAGACACACACACACAACACAC 162 22 0 50.00 61.316 0.00 0.00 0.00 3.316 70 GACACACACACACAACACACAG 160 22 0 50.00 61.316 0.00 0.00 0.00 3.316 71 CACACACACACAACACACAGTC 158 22 0 50.00 61.316 0.00 0.00 0.00 3.316 72 AGCTACATCCATACACAACACAC 132 23 0 43.48 59.675 0.00 0.00 0.00 3.325 73 ACACACAGTCAAGAAGCTACATC 146 23 0 43.48 59.615 0.00 0.00 0.00 3.385 74 CACACACACACAACATACACT 222 21 0 42.86 57.600 0.00 0.00 0.00 3.400 75 ACAGTCAAGAAGCTACATCCA 142 21 0 42.86 57.597 0.00 0.00 0.00 3.403 76 ACACACAGTCAAGAAGCTACAT 146 22 0 40.91 58.590 0.00 0.00 0.00 3.410 77 TGCACAATACCACACATGTTT 102 21 0 38.10 57.554 0.00 0.00 0.00 3.446 78 ACATCCATACACAACACACATGT 128 23 0 39.13 59.542 0.00 0.00 42.88 3.458 79 ACACAACACACAGTCAAGAAG 151 21 0 42.86 57.527 0.00 0.00 0.00 3.473 80 ACACACAACACACAGTCAAGAAG 153 23 0 43.48 60.486 0.00 0.00 0.00 3.486 81 CAACACACAGTCAAGAAGCTAC 148 22 0 45.45 58.445 0.00 0.00 0.00 3.555 82 CACACACACACAACACACA 158 19 0 47.37 57.435 0.00 0.00 0.00 3.565 83 ACACACACACACAACACAC 159 19 0 47.37 57.434 0.00 0.00 0.00 3.566 84 CACAGTCAAGAAGCTACATCCAT 143 23 0 43.48 59.421 0.00 0.00 0.00 3.579 85 AGCTACATCCATACACAACACA 132 22 0 40.91 58.384 0.00 0.00 0.00 3.616 86 ACACAGTCAAGAAGCTACATCCA 144 23 0 43.48 60.620 0.00 0.00 0.00 3.620 87 ATGCACAATACCACACATGTT 103 21 0 38.10 57.337 0.00 0.00 0.00 3.663 88 ACACACACACAACATACACTC 221 21 0 42.86 57.327 0.00 0.00 0.00 3.673 89 ATACACAACAGACACACACAC 170 21 0 42.86 57.327 0.00 0.00 0.00 3.673 90 GCTACATCCATACACAACACAC 131 22 0 45.45 58.308 0.00 0.00 0.00 3.692 91 CACACACAACACACAGTCAAGAA 154 23 0 43.48 60.732 0.00 0.00 0.00 3.732 92 CACAGTCAAGAAGCTACATCC 143 21 0 47.62 57.263 0.00 0.00 0.00 3.737 93 ACACAACAGACACACACACACA 168 22 0 45.45 61.737 0.00 0.00 0.00 3.737 94 ACAGACACACACACACAACACA 163 22 0 45.45 61.737 0.00 0.00 0.00 3.737 95 AGACACACACACACAACACACA 161 22 0 45.45 61.737 0.00 0.00 0.00 3.737 96 ACACACACACAACACACAGTCA 157 22 0 45.45 61.737 0.00 0.00 0.00 3.737 97 AACACACAGTCAAGAAGCTACAT 147 23 0 39.13 59.228 0.00 0.00 0.00 3.772 98 TACACACACACACAACATACACT 224 23 0 39.13 59.170 0.00 0.00 0.00 3.830 99 GACACACACACACAACACA 160 19 0 47.37 57.137 0.00 0.00 0.00 3.863 100 ACACACACACAACACACAG 157 19 0 47.37 57.128 0.00 0.00 0.00 3.872 101 CACACACACAACACACAGT 156 19 0 47.37 57.128 0.00 0.00 0.00 3.872 102 ACAGTCAAGAAGCTACATCCAT 142 22 0 40.91 58.107 0.00 0.00 0.00 3.893 103 ATGCACAATACCACACATGTTT 103 22 0 36.36 58.061 0.00 0.00 0.00 3.939 104 GATCTACACACACACACAACA 228 21 0 42.86 57.055 0.00 0.00 0.00 3.945 105 CACACACACAACATACACTCT 220 21 0 42.86 57.047 0.00 0.00 0.00 3.953 106 CAACACACAGTCAAGAAGCTA 148 21 0 42.86 57.047 0.00 0.00 0.00 3.953 107 AACACACAGTCAAGAAGCTAC 147 21 0 42.86 57.045 0.00 0.00 0.00 3.955 108 AAGCTACATCCATACACAACACA 133 23 0 39.13 59.034 0.00 0.00 0.00 3.966 109 ACACACACACACAACACACAGT 159 22 0 45.45 61.997 0.00 0.00 0.00 3.997 110 GAAGCTACATCCATACACAACACA 134 24 0 41.67 59.997 0.00 0.00 0.00 4.003 111 CACACAGTCAAGAAGCTACATC 145 22 0 45.45 57.988 0.00 0.00 0.00 4.012 112 TCTACACACACACACAACATACA 226 23 0 39.13 58.919 0.00 0.00 0.00 4.081 113 TATACACAACAGACACACACACA 171 23 0 39.13 58.919 0.00 0.00 0.00 4.081 114 ACATCCATACACAACACACATG 128 22 0 40.91 57.887 0.00 0.00 0.00 4.113 115 CATCCATACACAACACACATGT 127 22 0 40.91 57.887 0.00 0.00 42.88 4.113 116 CTACACACACACACAACATACACT 225 24 0 41.67 60.118 0.00 0.00 0.00 4.118 117 TCTACACACACACACAACATACAC 226 24 0 41.67 60.118 0.00 0.00 0.00 4.118 118 TACACACACACACAACATACACTC 224 24 0 41.67 60.118 0.00 0.00 0.00 4.118 119 TATACACAACAGACACACACACAC 171 24 0 41.67 60.118 0.00 0.00 0.00 4.118 120 CACACACACACAACATACACTCTA 222 24 0 41.67 59.879 0.00 0.00 0.00 4.121 121 CTACACACACACACAACATACAC 225 23 0 43.48 58.829 0.00 0.00 0.00 4.171 122 ACACAACACACATGTATATGCACA 120 24 0 37.50 60.177 0.00 0.00 39.26 4.177 123 AACACACAGTCAAGAAGCTACATC 147 24 0 41.67 60.177 0.00 0.00 0.00 4.177 124 TACACACACACACAACATACAC 224 22 0 40.91 57.783 0.00 0.00 0.00 4.217 125 AAGCTACATCCATACACAACACAC 133 24 0 41.67 60.237 0.00 0.00 0.00 4.237 126 AGAAGCTACATCCATACACAACAC 135 24 0 41.67 59.756 0.00 0.00 0.00 4.244 127 GCTACATCCATACACAACACACAT 131 24 0 41.67 60.297 0.00 0.00 0.00 4.297 128 ACACACACACAACATACACTCTA 221 23 0 39.13 58.662 0.00 0.00 0.00 4.338 129 ACACACACACAACATACACTCTAG 221 24 0 41.67 59.639 0.00 0.00 0.00 4.361 130 CACAACACACATGTATATGCACA 119 23 0 39.13 58.624 0.00 0.00 39.26 4.376 131 ACACAACACACATGTATATGCAC 120 23 0 39.13 58.623 0.00 0.00 39.26 4.377 132 CAACACACAGTCAAGAAGCTACAT 148 24 0 41.67 60.416 0.00 0.00 0.00 4.416 133 GATCTACACACACACACAACAT 228 22 0 40.91 57.574 0.00 0.00 0.00 4.426 134 AGAAGCTACATCCATACACAACA 135 23 0 39.13 58.521 0.00 0.00 0.00 4.479 135 CTACACACACACACAACATACA 225 22 0 40.91 57.517 0.00 0.00 0.00 4.483 136 GTCAAGAAGCTACATCCATACACA 139 24 0 41.67 59.515 0.00 0.00 0.00 4.485 137 TACACAACAGACACACACACACA 169 23 0 43.48 61.487 0.00 0.00 0.00 4.487 138 TGCACAATACCACACATGTTTA 102 22 0 36.36 57.471 0.00 0.00 0.00 4.529 139 GAAGCTACATCCATACACAACAC 134 23 0 43.48 58.444 0.00 0.00 0.00 4.556 140 CACACACACAACACACAGTCAAG 156 23 0 47.83 61.577 0.00 0.00 0.00 4.577 141 TGCACAATACCACACATGTTTAAC 102 24 0 37.50 59.401 0.00 0.00 0.00 4.599 142 GCACAATACCACACATGTTTAACA 101 24 0 37.50 59.401 0.00 0.00 0.00 4.599 143 CACACACACAACATACACTCTAGA 220 24 0 41.67 59.399 0.00 0.00 0.00 4.601 144 CACACACAACATACACTCTAGACA 218 24 0 41.67 59.399 0.00 0.00 0.00 4.601 145 CACACAACATACACTCTAGACACA 216 24 0 41.67 59.399 0.00 0.00 0.00 4.601 146 ACACACACAACATACACTCTAGAC 219 24 0 41.67 59.399 0.00 0.00 0.00 4.601 147 ACACACAACATACACTCTAGACAC 217 24 0 41.67 59.399 0.00 0.00 0.00 4.601 148 TACATCCATACACAACACACATGT 129 24 0 37.50 59.387 0.00 0.00 42.88 4.613 149 ACATCCATACACAACACACATGTA 128 24 0 37.50 59.387 0.00 0.00 39.26 4.613 150 CACACAACACACAGTCAAGAAGC 152 23 0 47.83 61.639 0.00 0.00 0.00 4.639 151 AAGCTACATCCATACACAACAC 133 22 0 40.91 57.359 0.00 0.00 0.00 4.641 152 ATCTACACACACACACAACATACA 227 24 0 37.50 59.331 0.00 0.00 0.00 4.669 153 ATATACACAACAGACACACACACA 172 24 0 37.50 59.331 0.00 0.00 0.00 4.669 154 ACACAACACACATGTATATGCA 120 22 0 36.36 57.281 0.00 0.00 39.26 4.719 155 ACAACACACATGTATATGCACA 118 22 0 36.36 57.281 0.00 0.00 39.26 4.719 156 CACAGTCAAGAAGCTACATCCATA 143 24 0 41.67 59.271 0.00 0.00 0.00 4.729 157 CAGTCAAGAAGCTACATCCATACA 141 24 0 41.67 59.271 0.00 0.00 0.00 4.729 158 ACAGTCAAGAAGCTACATCCATAC 142 24 0 41.67 59.271 0.00 0.00 0.00 4.729 159 AGTCAAGAAGCTACATCCATACAC 140 24 0 41.67 59.271 0.00 0.00 0.00 4.729 160 TCAAGAAGCTACATCCATACACA 138 23 0 39.13 58.265 0.00 0.00 0.00 4.735 161 TATGCACAATACCACACATGTT 104 22 0 36.36 57.263 0.00 0.00 0.00 4.737 162 ACACACACAACACACAGTCAAGA 155 23 0 43.48 61.740 0.00 0.00 0.00 4.740 163 TCTACACACACACACAACATAC 226 22 0 40.91 57.255 0.00 0.00 0.00 4.745 164 TATACACAACAGACACACACAC 171 22 0 40.91 57.255 0.00 0.00 0.00 4.745 165 CACAACACACATGTATATGCACAA 119 24 0 37.50 59.225 0.00 0.00 39.26 4.775 166 ACACAACACACAGTCAAGAAGCT 151 23 0 43.48 61.808 0.00 0.00 0.00 4.808 167 CACAACAGACACACACACACAAC 167 23 0 47.83 61.817 0.00 0.00 0.00 4.817 168 CAACAGACACACACACACAACAC 165 23 0 47.83 61.817 0.00 0.00 0.00 4.817 169 TGCACAATACCACACATGTTTAA 102 23 0 34.78 58.157 0.00 0.00 0.00 4.843 170 ACACACACAACATACACTCTAGA 219 23 0 39.13 58.151 0.00 0.00 0.00 4.849 171 ACACACAACATACACTCTAGACA 217 23 0 39.13 58.151 0.00 0.00 0.00 4.849 172 ACACAACATACACTCTAGACACA 215 23 0 39.13 58.151 0.00 0.00 0.00 4.849 173 AAGAAGCTACATCCATACACAACA 136 24 0 37.50 59.140 0.00 0.00 0.00 4.860 174 GAAGCTACATCCATACACAACA 134 22 0 40.91 57.096 0.00 0.00 0.00 4.904 175 CACACACACAACATACACTCTAG 220 23 0 43.48 58.081 0.00 0.00 0.00 4.919 176 ATATGCACAATACCACACATGT 105 22 0 36.36 57.054 0.00 0.00 0.00 4.946 177 CTACATCCATACACAACACACA 130 22 0 40.91 57.033 0.00 0.00 0.00 4.967 178 ACACAGTCAAGAAGCTACATCCAT 144 24 0 41.67 60.971 0.00 0.00 0.00 4.971 179 ACAGTCAAGAAGCTACATCCATA 142 23 0 39.13 58.004 0.00 0.00 0.00 4.996 180 AGTCAAGAAGCTACATCCATACA 140 23 0 39.13 58.004 0.00 0.00 0.00 4.996 181 TACACAACACACATGTATATGCACA 121 25 0 36.00 60.005 0.00 0.00 39.26 5.005 182 ACAACACACATGTATATGCACAA 118 23 0 34.78 57.973 0.00 0.00 39.26 5.027 183 TATGCACAATACCACACATGTTT 104 23 0 34.78 57.961 0.00 0.00 0.00 5.039 184 ATGCACAATACCACACATGTTTA 103 23 0 34.78 57.961 0.00 0.00 0.00 5.039 185 GTCAAGAAGCTACATCCATACAC 139 23 0 43.48 57.941 0.00 0.00 0.00 5.059 186 GTCAAGAAGCTACATCCATACACAA 139 25 0 40.00 60.063 0.00 0.00 0.00 5.063 187 TCAAGAAGCTACATCCATACACAAC 138 25 0 40.00 60.063 0.00 0.00 0.00 5.063 188 TCAAGAAGCTACATCCATACACAA 138 24 0 37.50 58.896 0.00 0.00 0.00 5.104 189 GCACAATACCACACATGTTTAAC 101 23 0 39.13 57.839 0.00 0.00 0.00 5.161 190 CACACACAACATACACTCTAGAC 218 23 0 43.48 57.832 0.00 0.00 0.00 5.168 191 CACACAACATACACTCTAGACAC 216 23 0 43.48 57.832 0.00 0.00 0.00 5.168 192 ACACACATGTATATGCACAATACCA 115 25 0 36.00 59.828 15.76 7.60 38.02 5.172 193 ACACATGTATATGCACAATACCACA 113 25 0 36.00 59.828 15.76 4.59 38.43 5.172 194 ACATGTATATGCACAATACCACACA 111 25 0 36.00 59.828 15.76 4.01 38.43 5.172 195 TGTATATGCACAATACCACACATGT 108 25 0 36.00 59.828 15.76 0.00 0.00 5.172 196 CACAACACACAGTCAAGAAGCTAC 150 24 0 45.83 61.177 0.00 0.00 0.00 5.177 197 CTACATCCATACACAACACACATG 130 24 0 41.67 58.810 0.00 0.00 0.00 5.190 198 CAAGAAGCTACATCCATACACAAC 137 24 0 41.67 58.809 0.00 0.00 0.00 5.191 199 TACATCCATACACAACACACATG 129 23 0 39.13 57.795 0.00 0.00 0.00 5.205 200 CATCCATACACAACACACATGTA 127 23 0 39.13 57.795 0.00 0.00 39.26 5.205 201 AGCTACATCCATACACAACACACA 132 24 0 41.67 61.209 0.00 0.00 0.00 5.209 202 ATGCACAATACCACACATGTTTAAC 103 25 0 36.00 59.776 0.00 0.00 0.00 5.224 203 CACACAACATACACTCTAGACACAT 216 25 0 40.00 59.775 0.00 0.00 0.00 5.225 204 ACATCCATACACAACACACATGTAT 128 25 0 36.00 59.769 0.00 0.00 39.26 5.231 205 ACACAACAGACACACACACACAA 168 23 0 43.48 62.233 0.00 0.00 0.00 5.233 206 ACAACAGACACACACACACAACA 166 23 0 43.48 62.233 0.00 0.00 0.00 5.233 207 AACAGACACACACACACAACACA 164 23 0 43.48 62.233 0.00 0.00 0.00 5.233 208 ACACACACACAACACACAGTCAA 157 23 0 43.48 62.233 0.00 0.00 0.00 5.233 209 GATCTACACACACACACAACATACA 228 25 0 40.00 60.236 0.00 0.00 0.00 5.236 210 ATATGCACAATACCACACATGTT 105 23 0 34.78 57.763 0.00 0.00 0.00 5.237 211 GATCTACACACACACACAACATAC 228 24 0 41.67 58.761 0.00 0.00 0.00 5.239 212 ATCTACACACACACACAACATAC 227 23 0 39.13 57.745 0.00 0.00 0.00 5.255 213 ATATACACAACAGACACACACAC 172 23 0 39.13 57.745 0.00 0.00 0.00 5.255 214 GCACAATACCACACATGTTTAACAG 101 25 0 40.00 60.292 0.00 0.00 0.00 5.292 215 CAAGAAGCTACATCCATACACAACA 137 25 0 40.00 60.294 0.00 0.00 0.00 5.294 216 CTACATCCATACACAACACACATGT 130 25 0 40.00 60.294 0.00 0.00 42.88 5.294 217 AAGAAGCTACATCCATACACAACAC 136 25 0 40.00 60.294 0.00 0.00 0.00 5.294 218 CAGTCAAGAAGCTACATCCATAC 141 23 0 43.48 57.686 0.00 0.00 0.00 5.314 219 CACAACACACATGTATATGCACAAT 119 25 0 36.00 59.605 0.00 0.00 39.26 5.395 220 ATGCACAATACCACACATGTTTAA 103 24 0 33.33 58.600 0.00 0.00 0.00 5.400 221 ACACAACATACACTCTAGACACAT 215 24 0 37.50 58.595 0.00 0.00 0.00 5.405 222 CACAGTCAAGAAGCTACATCCATAC 143 25 0 44.00 60.408 0.00 0.00 0.00 5.408 223 CAGTCAAGAAGCTACATCCATACAC 141 25 0 44.00 60.408 0.00 0.00 0.00 5.408 224 CAAGAAGCTACATCCATACACAA 137 23 0 39.13 57.536 0.00 0.00 0.00 5.464 225 CTACATCCATACACAACACACAT 130 23 0 39.13 57.536 0.00 0.00 0.00 5.464 226 AAGAAGCTACATCCATACACAAC 136 23 0 39.13 57.535 0.00 0.00 0.00 5.465 227 ATCTACACACACACACAACATACAC 227 25 0 40.00 60.466 0.00 0.00 0.00 5.466 228 ATATACACAACAGACACACACACAC 172 25 0 40.00 60.466 0.00 0.00 0.00 5.466 229 TACACAACACACATGTATATGCAC 121 24 0 37.50 58.507 0.00 0.00 39.26 5.493 230 GATCTACACACACACACAACATA 228 23 0 39.13 57.495 0.00 0.00 0.00 5.505 231 CCATACACAACACACATGTATATGC 124 25 0 40.00 59.494 2.84 0.00 39.26 5.506 232 CACACATGTATATGCACAATACCAC 114 25 0 40.00 59.494 15.76 0.00 38.43 5.506 233 CACATGTATATGCACAATACCACAC 112 25 0 40.00 59.494 15.76 0.00 38.43 5.506 234 CACACACACAACATACACTCTAGAC 220 25 0 44.00 60.520 0.00 0.00 0.00 5.520 235 CACACACAACATACACTCTAGACAC 218 25 0 44.00 60.520 0.00 0.00 0.00 5.520 236 ACACACACACACAACATACACTCT 223 24 0 41.67 61.564 0.00 0.00 0.00 5.564 237 ACACAACACACAGTCAAGAAGCTA 151 24 0 41.67 61.564 0.00 0.00 0.00 5.564 238 ACAACACACAGTCAAGAAGCTACA 149 24 0 41.67 61.564 0.00 0.00 0.00 5.564 239 ACAACACACATGTATATGCACAAT 118 24 0 33.33 58.422 0.00 0.00 39.26 5.578 240 AGACACACACACACAACACACAG 161 23 0 47.83 62.582 0.00 0.00 0.00 5.582 241 CACACACAACACACAGTCAAGAAG 154 24 0 45.83 61.586 0.00 0.00 0.00 5.586 242 ATATGCACAATACCACACATGTTT 105 24 0 33.33 58.412 0.00 0.00 0.00 5.588 243 CACACATGTATATGCACAATACCA 114 24 0 37.50 58.313 15.76 7.60 38.43 5.687 244 CACATGTATATGCACAATACCACA 112 24 0 37.50 58.313 15.76 4.59 38.43 5.687 245 CATGTATATGCACAATACCACACA 110 24 0 37.50 58.313 15.76 4.01 31.46 5.687 246 TGTATATGCACAATACCACACATG 108 24 0 37.50 58.313 15.76 0.00 0.00 5.687 247 ACACACATGTATATGCACAATACC 115 24 0 37.50 58.312 15.76 3.34 38.02 5.688 248 ACACATGTATATGCACAATACCAC 113 24 0 37.50 58.312 15.76 0.00 38.43 5.688 249 ACATGTATATGCACAATACCACAC 111 24 0 37.50 58.312 15.76 0.00 38.43 5.688 250 GTATATGCACAATACCACACATGT 107 24 0 37.50 58.312 9.75 0.00 0.00 5.688 251 ACACAACACACATGTATATGCACAA 120 25 0 36.00 60.700 0.00 0.00 39.26 5.700 252 CACACAGTCAAGAAGCTACATCCA 145 24 0 45.83 61.738 0.00 0.00 0.00 5.738 253 ACACACAGTCAAGAAGCTACATCC 146 24 0 45.83 61.739 0.00 0.00 0.00 5.739 254 CACACACACACAACATACACTCTAG 222 25 0 44.00 60.746 0.00 0.00 0.00 5.746 255 CATCCATACACAACACACATGTAT 127 24 0 37.50 58.249 0.00 0.00 39.26 5.751 256 TACATCCATACACAACACACATGTA 129 25 0 36.00 59.243 0.00 0.00 39.26 5.757 257 ACACAGTCAAGAAGCTACATCCATA 144 25 0 40.00 60.765 0.00 0.00 0.00 5.765 258 ACAGTCAAGAAGCTACATCCATACA 142 25 0 40.00 60.765 0.00 0.00 0.00 5.765 259 AGTCAAGAAGCTACATCCATACACA 140 25 0 40.00 60.765 0.00 0.00 0.00 5.765 260 TACACAACACACATGTATATGCA 121 23 0 34.78 57.213 0.00 0.00 39.26 5.787 261 ATACACAACAGACACACACACACA 170 24 0 41.67 61.800 0.00 0.00 0.00 5.800 262 TGTATATACACAACAGACACACACA 175 25 0 36.00 59.190 16.53 0.00 0.00 5.810 263 TATATACACAACAGACACACACACA 173 25 0 36.00 59.190 0.00 0.00 0.00 5.810 264 CAGACACACACACACAACACACA 162 23 0 47.83 62.820 0.00 0.00 0.00 5.820 265 CACACACACACAACACACAGTCA 158 23 0 47.83 62.820 0.00 0.00 0.00 5.820 266 ACAGACACACACACACAACACAC 163 23 0 47.83 62.822 0.00 0.00 0.00 5.822 267 GACACACACACACAACACACAGT 160 23 0 47.83 62.822 0.00 0.00 0.00 5.822 268 ACACACACACACAACACACAGTC 159 23 0 47.83 62.822 0.00 0.00 0.00 5.822 269 TGCACAATACCACACATGTTTAACA 102 25 0 36.00 60.875 0.00 0.00 0.00 5.875 270 ACACACACACAACATACACTCTAGA 221 25 0 40.00 60.877 0.00 0.00 0.00 5.877 271 ACACACACAACATACACTCTAGACA 219 25 0 40.00 60.877 0.00 0.00 0.00 5.877 272 ACACACAACATACACTCTAGACACA 217 25 0 40.00 60.877 0.00 0.00 0.00 5.877 273 CTACACACACACACAACATACACTC 225 25 0 44.00 60.972 0.00 0.00 0.00 5.972 274 TACACAACAGACACACACACACAA 169 24 0 41.67 61.974 0.00 0.00 0.00 5.974 275 CAACACACATGTATATGCACAATACC 117 26 0 38.46 60.015 15.76 3.34 39.26 6.015 276 CATACACAACACACATGTATATGCAC 123 26 0 38.46 60.016 0.00 0.00 39.26 6.016 277 CACAATACCACACATGTTTAACAGA 100 25 0 36.00 58.967 3.60 0.00 0.00 6.033 278 CACAATACCACACATGTTTAACAGAC 100 26 0 38.46 60.070 3.60 0.00 0.00 6.070 279 CATACACAACACACATGTATATGCA 123 25 0 36.00 58.914 0.00 0.00 39.26 6.086 280 ATACACAACACACATGTATATGCAC 122 25 0 36.00 58.914 0.00 0.00 39.26 6.086 281 GAAGCTACATCCATACACAACACAC 134 25 0 44.00 61.090 0.00 0.00 0.00 6.090 282 AACACACATGTATATGCACAATACC 116 25 0 36.00 58.907 15.76 3.34 39.26 6.093 283 GTATATGCACAATACCACACATGTT 107 25 0 36.00 58.907 9.75 0.00 0.00 6.093 284 CTACATCCATACACAACACACATGTA 130 26 0 38.46 60.124 0.00 0.00 39.26 6.124 285 GTATATACACAACAGACACACACAC 174 25 0 40.00 58.872 8.63 0.00 0.00 6.128 286 TATGCACAATACCACACATGTTTA 104 24 0 33.33 57.868 0.00 0.00 0.00 6.132 287 CATGTATATGCACAATACCACACATG 110 26 0 38.46 59.850 12.26 9.17 31.83 6.150 288 ACATGTATATGCACAATACCACACAT 111 26 0 34.62 60.180 15.76 5.82 38.43 6.180 289 ATGTATATGCACAATACCACACATGT 109 26 0 34.62 60.180 15.76 0.00 31.46 6.180 290 ACACACACAACACACAGTCAAGAA 155 24 0 41.67 62.217 0.00 0.00 0.00 6.217 291 AGAAGCTACATCCATACACAACACA 135 25 0 40.00 61.231 0.00 0.00 0.00 6.231 292 CAACACACAGTCAAGAAGCTACATC 148 25 0 44.00 61.257 0.00 0.00 0.00 6.257 293 CATGTATATGCACAATACCACACAT 110 25 0 36.00 58.730 15.76 5.82 31.46 6.270 294 ATGTATATGCACAATACCACACATG 109 25 0 36.00 58.730 15.76 0.00 31.46 6.270 295 TGTATATACACAACAGACACACACAC 175 26 0 38.46 60.291 16.53 0.00 0.00 6.291 296 GTATATACACAACAGACACACACACA 174 26 0 38.46 60.291 8.63 0.00 0.00 6.291 297 TATATACACAACAGACACACACACAC 173 26 0 38.46 60.291 0.00 0.00 0.00 6.291 298 ATACACAACACACATGTATATGCA 122 24 0 33.33 57.692 0.00 0.00 39.26 6.308 299 CACAATACCACACATGTTTAACAG 100 24 0 37.50 57.686 0.00 0.00 0.00 6.314 300 TATATGCACAATACCACACATGTT 106 24 0 33.33 57.678 0.00 0.00 0.00 6.322 301 TGTATATACACAACAGACACACAC 175 24 0 37.50 57.663 16.53 0.00 0.00 6.337 302 GTATATACACAACAGACACACACA 174 24 0 37.50 57.663 8.63 0.00 0.00 6.337 303 TATATACACAACAGACACACACAC 173 24 0 37.50 57.663 0.00 0.00 0.00 6.337 304 TCTACACACACACACAACATACACT 226 25 0 40.00 61.338 0.00 0.00 0.00 6.338 305 TACACACACACACAACATACACTCT 224 25 0 40.00 61.338 0.00 0.00 0.00 6.338 306 ACACACACACACAACATACACTCTA 223 25 0 40.00 61.338 0.00 0.00 0.00 6.338 307 ATACACAACACACATGTATATGCACA 122 26 0 34.62 60.348 0.00 0.00 39.26 6.348 308 AACACACATGTATATGCACAATACCA 116 26 0 34.62 60.349 15.76 7.60 39.26 6.349 309 TGTATATGCACAATACCACACATGTT 108 26 0 34.62 60.349 15.76 0.00 0.00 6.349 310 GCTACATCCATACACAACACACATG 131 25 0 44.00 61.374 0.00 0.00 0.00 6.374 311 TATGCACAATACCACACATGTTTAAC 104 26 0 34.62 59.626 0.00 0.00 0.00 6.374 312 TACATCCATACACAACACACATGTAT 129 26 0 34.62 59.616 0.00 0.00 39.26 6.384 313 ACATCCATACACAACACACATGTATA 128 26 0 34.62 59.616 0.00 0.00 39.26 6.384 314 TATGCACAATACCACACATGTTTAA 104 25 0 32.00 58.487 0.00 0.00 0.00 6.513 315 ATGTATATGCACAATACCACACAT 109 24 0 33.33 57.486 15.76 5.82 31.46 6.514 316 TACACAACACACATGTATATGCACAA 121 26 0 34.62 60.515 0.00 0.00 39.26 6.515 317 AGCTACATCCATACACAACACACAT 132 25 0 40.00 61.523 0.00 0.00 0.00 6.523 318 CACAACACACATGTATATGCACAATA 119 26 0 34.62 59.461 0.00 0.00 39.26 6.539 319 ACAACACACATGTATATGCACAATAC 118 26 0 34.62 59.461 12.84 12.84 39.26 6.539 320 GTATATGCACAATACCACACATGTTT 107 26 0 34.62 59.457 9.75 0.00 0.00 6.543 321 TATACACAACAGACACACACACACA 171 25 0 40.00 61.566 0.00 0.00 0.00 6.566 322 CATACACAACACACATGTATATGC 123 24 0 37.50 57.398 0.00 0.00 39.26 6.602 323 TCCATACACAACACACATGTATATGC 125 26 0 38.46 60.680 2.84 0.00 39.26 6.680 324 ACAACACACATGTATATGCACAATA 118 25 0 32.00 58.316 0.00 0.00 39.26 6.684 325 TATATGCACAATACCACACATGTTT 106 25 0 32.00 58.306 0.00 0.00 0.00 6.694 326 ATATGCACAATACCACACATGTTTA 105 25 0 32.00 58.306 0.00 0.00 0.00 6.694 327 AAGCTACATCCATACACAACACACA 133 25 0 40.00 61.694 0.00 0.00 0.00 6.694 328 CACACACACAACACACAGTCAAGA 156 24 0 45.83 62.783 0.00 0.00 0.00 6.783 329 CACACAACACACAGTCAAGAAGCT 152 24 0 45.83 62.850 0.00 0.00 0.00 6.850 330 CATCCATACACAACACACATGTATA 127 25 0 36.00 58.150 0.00 0.00 39.26 6.850 331 TCCATACACAACACACATGTATATG 125 25 0 36.00 58.150 1.80 1.80 39.26 6.850 332 ACAACACACAGTCAAGAAGCTACAT 149 25 0 40.00 61.863 0.00 0.00 0.00 6.863 333 CCATACACAACACACATGTATATGCA 124 26 0 38.46 60.902 2.84 0.00 39.26 6.902 334 CACACATGTATATGCACAATACCACA 114 26 0 38.46 60.902 15.76 4.59 38.43 6.902 335 CACATGTATATGCACAATACCACACA 112 26 0 38.46 60.902 15.76 4.01 38.43 6.902 336 ACACACATGTATATGCACAATACCAC 115 26 0 38.46 60.902 15.76 0.00 38.02 6.902 337 ACACATGTATATGCACAATACCACAC 113 26 0 38.46 60.902 15.76 0.00 38.43 6.902 338 CAACACACATGTATATGCACAATAC 117 25 0 36.00 58.019 12.84 12.84 39.26 6.981 339 ACACAACACACATGTATATGCACAAT 120 26 0 34.62 61.018 0.00 0.00 39.26 7.018 340 CACACAGTCAAGAAGCTACATCCAT 145 25 0 44.00 62.029 0.00 0.00 0.00 7.029 341 ATATGCACAATACCACACATGTTTAAC 105 27 0 33.33 59.967 0.00 0.00 0.00 7.033 342 ACATCCATACACAACACACATGTATAT 128 27 0 33.33 59.963 0.00 0.00 39.26 7.037 343 ATATGCACAATACCACACATGTTTAA 105 26 0 30.77 58.886 0.00 0.00 0.00 7.114 344 GTCAAGAAGCTACATCCATACACAAC 139 26 0 42.31 61.115 0.00 0.00 0.00 7.115 345 TACACACACACACAACATACACTCTA 224 26 0 38.46 61.130 0.00 0.00 0.00 7.130 346 ATGCACAATACCACACATGTTTAACA 103 26 0 34.62 61.187 0.00 0.00 0.00 7.187 347 ACACACAACATACACTCTAGACACAT 217 26 0 38.46 61.190 0.00 0.00 0.00 7.190 348 AACACACAGTCAAGAAGCTACATCC 147 25 0 44.00 62.198 0.00 0.00 0.00 7.198 349 AGTCAAGAAGCTACATCCATACACAA 140 26 0 38.46 61.252 0.00 0.00 0.00 7.252 350 ATACACAACAGACACACACACACAA 170 25 0 40.00 62.257 0.00 0.00 0.00 7.257 351 GATCTACACACACACACAACATACAC 228 26 0 42.31 61.271 0.00 0.00 0.00 7.271 352 CAAGAAGCTACATCCATACACAACAC 137 26 0 42.31 61.334 0.00 0.00 0.00 7.334 353 CATCCATACACAACACACATGTATATG 127 27 0 37.04 59.649 1.80 1.80 39.26 7.351 354 CATCCATACACAACACACATGTATAT 127 26 0 34.62 58.558 0.00 0.00 39.26 7.442 355 ATCCATACACAACACACATGTATATG 126 26 0 34.62 58.558 1.80 1.80 39.26 7.442 356 CTACATCCATACACAACACACATGTAT 130 27 0 37.04 60.450 0.00 0.00 39.26 7.450 357 GCACAATACCACACATGTTTAACAGA 101 26 0 38.46 61.452 3.60 0.00 0.00 7.452 358 TCAAGAAGCTACATCCATACACAACA 138 26 0 38.46 61.474 0.00 0.00 0.00 7.474 359 CACAACACACATGTATATGCACAATAC 119 27 0 37.04 60.497 12.84 12.84 39.26 7.497 360 TACATCCATACACAACACACATGTATA 129 27 0 33.33 59.474 0.00 0.00 39.26 7.526 361 CACACAACACACAGTCAAGAAGCTA 152 25 0 44.00 62.579 0.00 0.00 0.00 7.579 362 CACAACACACAGTCAAGAAGCTACA 150 25 0 44.00 62.579 0.00 0.00 0.00 7.579 363 ACACAACACACAGTCAAGAAGCTAC 151 25 0 44.00 62.581 0.00 0.00 0.00 7.581 364 ATCTACACACACACACAACATACACT 227 26 0 38.46 61.634 0.00 0.00 0.00 7.634 365 ATCCATACACAACACACATGTATAT 126 25 0 32.00 57.351 0.00 0.00 39.26 7.649 366 ATGTATATGCACAATACCACACATGTT 109 27 0 33.33 60.670 15.76 0.00 31.46 7.670 367 TGCACAATACCACACATGTTTAACAG 102 26 0 38.46 61.672 0.00 0.00 0.00 7.672 368 GTATATGCACAATACCACACATGTTTA 107 27 0 33.33 59.323 9.75 0.00 0.00 7.677 369 AAGAAGCTACATCCATACACAACACA 136 26 0 38.46 61.698 0.00 0.00 0.00 7.698 370 TATATGCACAATACCACACATGTTTA 106 26 0 30.77 58.208 0.00 0.00 0.00 7.792 371 CACACAGTCAAGAAGCTACATCCATA 145 26 0 42.31 61.796 0.00 0.00 0.00 7.796 372 CACAGTCAAGAAGCTACATCCATACA 143 26 0 42.31 61.796 0.00 0.00 0.00 7.796 373 CAGTCAAGAAGCTACATCCATACACA 141 26 0 42.31 61.796 0.00 0.00 0.00 7.796 374 ACACAGTCAAGAAGCTACATCCATAC 144 26 0 42.31 61.797 0.00 0.00 0.00 7.797 375 ACAGTCAAGAAGCTACATCCATACAC 142 26 0 42.31 61.797 0.00 0.00 0.00 7.797 376 ATACACAACACACATGTATATGCACAA 122 27 0 33.33 60.828 0.00 0.00 39.26 7.828 377 TACACAACACACATGTATATGCACAAT 121 27 0 33.33 60.828 0.00 0.00 39.26 7.828 378 ACACAACACACATGTATATGCACAATA 120 27 0 33.33 60.828 0.00 0.00 39.26 7.828 379 TGTATATGCACAATACCACACATGTTT 108 27 0 33.33 60.831 15.76 0.00 0.00 7.831 380 ATATACACAACAGACACACACACACA 172 26 0 38.46 61.853 0.00 0.00 0.00 7.853 381 CACACACACACAACATACACTCTAGA 222 26 0 42.31 61.893 0.00 0.00 0.00 7.893 382 CACACACACAACATACACTCTAGACA 220 26 0 42.31 61.893 0.00 0.00 0.00 7.893 383 CACACACAACATACACTCTAGACACA 218 26 0 42.31 61.893 0.00 0.00 0.00 7.893 384 ACACACACACAACATACACTCTAGAC 221 26 0 42.31 61.894 0.00 0.00 0.00 7.894 385 ACACACACAACATACACTCTAGACAC 219 26 0 42.31 61.894 0.00 0.00 0.00 7.894 386 TACACAACAGACACACACACACAAC 169 25 0 44.00 62.964 0.00 0.00 0.00 7.964 387 ACACACACAACACACAGTCAAGAAG 155 25 0 44.00 62.976 0.00 0.00 0.00 7.976 388 AAGCTACATCCATACACAACACACAT 133 26 0 38.46 61.979 0.00 0.00 0.00 7.979 389 ATCCATACACAACACACATGTATATGC 126 27 0 37.04 60.986 2.84 0.00 39.26 7.986 390 TATGCACAATACCACACATGTTTAACA 104 27 0 33.33 60.991 0.00 0.00 0.00 7.991 391 TATACACAACAGACACACACACACAA 171 26 0 38.46 62.015 0.00 0.00 0.00 8.015 392 TCTACACACACACACAACATACACTC 226 26 0 42.31 62.110 0.00 0.00 0.00 8.110 393 CTACACACACACACAACATACACTCT 225 26 0 42.31 62.114 0.00 0.00 0.00 8.114 394 ACACACACACACAACATACACTCTAG 223 26 0 42.31 62.114 0.00 0.00 0.00 8.114 395 CACATGTATATGCACAATACCACACAT 112 27 0 37.04 61.200 15.76 5.82 38.43 8.200 396 ACATGTATATGCACAATACCACACATG 111 27 0 37.04 61.200 16.36 16.36 38.43 8.200 397 CATGTATATGCACAATACCACACATGT 110 27 0 37.04 61.200 14.06 0.00 33.01 8.200 398 TATATGCACAATACCACACATGTTTAA 106 27 0 29.63 58.771 0.00 0.00 0.00 8.229 399 AGAAGCTACATCCATACACAACACAC 135 26 0 42.31 62.236 0.00 0.00 0.00 8.236 400 GTATATACACAACAGACACACACACAC 174 27 0 40.74 61.287 8.63 0.00 0.00 8.287 401 CATACACAACACACATGTATATGCACA 123 27 0 37.04 61.352 0.00 0.00 39.26 8.352 402 CAACACACATGTATATGCACAATACCA 117 27 0 37.04 61.358 15.76 7.60 39.26 8.358 403 ACAACACACATGTATATGCACAATACC 118 27 0 37.04 61.358 15.76 3.34 39.26 8.358 404 AACACACATGTATATGCACAATACCAC 116 27 0 37.04 61.358 15.76 0.00 39.26 8.358 405 CACAATACCACACATGTTTAACAGACA 100 27 0 37.04 61.410 3.60 0.00 0.00 8.410 406 GAAGCTACATCCATACACAACACACA 134 26 0 42.31 62.452 0.00 0.00 0.00 8.452 407 AGCTACATCCATACACAACACACATG 132 26 0 42.31 62.512 0.00 0.00 0.00 8.512 408 ACAACACACAGTCAAGAAGCTACATC 149 26 0 42.31 62.610 0.00 0.00 0.00 8.610 409 TGTATATACACAACAGACACACACACA 175 27 0 37.04 61.634 16.53 0.00 0.00 8.634 410 TATATACACAACAGACACACACACACA 173 27 0 37.04 61.634 0.00 0.00 0.00 8.634 411 GCTACATCCATACACAACACACATGT 131 26 0 42.31 62.728 0.00 0.00 42.88 8.728 412 CACAACACACAGTCAAGAAGCTACAT 150 26 0 42.31 62.828 0.00 0.00 0.00 8.828 413 CCATACACAACACACATGTATATGCAC 124 27 0 40.74 61.873 2.84 0.00 39.26 8.873 414 CACACATGTATATGCACAATACCACAC 114 27 0 40.74 61.873 15.76 0.00 38.43 8.873 415 CTACACACACACACAACATACACTCTA 225 27 0 40.74 61.887 0.00 0.00 0.00 8.887 416 TACACACACACACAACATACACTCTAG 224 27 0 40.74 61.887 0.00 0.00 0.00 8.887 417 ATGCACAATACCACACATGTTTAACAG 103 27 0 37.04 61.943 0.00 0.00 0.00 8.943 418 TCCATACACAACACACATGTATATGCA 125 27 0 37.04 62.020 2.84 0.00 39.26 9.020 419 CACACACAACATACACTCTAGACACAT 218 27 0 40.74 62.157 0.00 0.00 0.00 9.157 420 CAGTCAAGAAGCTACATCCATACACAA 141 27 0 40.74 62.221 0.00 0.00 0.00 9.221 421 AGTCAAGAAGCTACATCCATACACAAC 140 27 0 40.74 62.222 0.00 0.00 0.00 9.222 422 ACACACATGTATATGCACAATACCACA 115 27 0 37.04 62.236 15.76 4.59 38.02 9.236 423 ACACATGTATATGCACAATACCACACA 113 27 0 37.04 62.236 15.76 4.01 38.43 9.236 424 ATATACACAACAGACACACACACACAA 172 27 0 37.04 62.277 0.00 0.00 0.00 9.277 425 GATCTACACACACACACAACATACACT 228 27 0 40.74 62.366 0.00 0.00 0.00 9.366 426 ATCTACACACACACACAACATACACTC 227 27 0 40.74 62.366 0.00 0.00 0.00 9.366 427 GCACAATACCACACATGTTTAACAGAC 101 27 0 40.74 62.394 3.60 0.00 0.00 9.394 428 GTCAAGAAGCTACATCCATACACAACA 139 27 0 40.74 62.430 0.00 0.00 0.00 9.430 429 TCAAGAAGCTACATCCATACACAACAC 138 27 0 40.74 62.430 0.00 0.00 0.00 9.430 430 GCTACATCCATACACAACACACATGTA 131 27 0 40.74 62.480 0.00 0.00 39.26 9.480 431 CAAGAAGCTACATCCATACACAACACA 137 27 0 40.74 62.643 0.00 0.00 0.00 9.643 432 AAGAAGCTACATCCATACACAACACAC 136 27 0 40.74 62.644 0.00 0.00 0.00 9.644 433 GAAGCTACATCCATACACAACACACAT 134 27 0 40.74 62.698 0.00 0.00 0.00 9.698 434 CACACAGTCAAGAAGCTACATCCATAC 145 27 0 44.44 62.728 0.00 0.00 0.00 9.728 435 CACAGTCAAGAAGCTACATCCATACAC 143 27 0 44.44 62.728 0.00 0.00 0.00 9.728 436 TGCACAATACCACACATGTTTAACAGA 102 27 0 37.04 62.765 3.60 0.00 0.00 9.765 437 CACACACACACAACATACACTCTAGAC 222 27 0 44.44 62.814 0.00 0.00 0.00 9.814 438 CACACACACAACATACACTCTAGACAC 220 27 0 44.44 62.814 0.00 0.00 0.00 9.814 439 AAGCTACATCCATACACAACACACATG 133 27 0 40.74 62.910 0.00 0.00 0.00 9.910 440 TATACACAACAGACACACACACACAAC 171 27 0 40.74 62.936 0.00 0.00 0.00 9.936 primer3-2.3.6/test/primer1_th_list_last/SP01-19.txt.rev0000664000077300007730000001411512231156243021433 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 CAGTGCTCTTTCTGCCAGTG 176 20 0 55.00 60.030 0.00 0.00 0.00 0.030 1 GTGCTCTTTCTGCCAGTGTC 174 20 0 55.00 60.030 0.00 0.00 0.00 0.030 2 GCTCTTTCTGCCAGTGTCAC 172 20 0 55.00 60.030 0.00 0.00 0.00 0.030 3 CATCCAGTGCTCTTTCTGCC 180 20 0 55.00 59.816 0.00 0.00 0.00 0.184 4 ATCCAGTGCTCTTTCTGCCA 179 20 0 50.00 60.243 0.00 0.00 0.00 0.243 5 TGCTCTTTCTGCCAGTGTCA 173 20 0 50.00 60.459 0.00 0.00 0.00 0.459 6 AGTGCTCTTTCTGCCAGTGT 175 20 0 50.00 60.460 0.00 0.00 0.00 0.460 7 GATCCCTCATCCAGTGCTCT 187 20 0 55.00 59.513 0.00 0.00 0.00 0.487 8 TCCCTCATCCAGTGCTCTTT 185 20 0 50.00 59.284 0.00 0.00 0.00 0.716 9 TCCAGTGCTCTTTCTGCCAG 178 20 0 55.00 60.888 0.00 0.00 0.00 0.888 10 ATCCCTCATCCAGTGCTCTT 186 20 0 50.00 59.058 0.00 0.00 0.00 0.942 11 CTCATCCAGTGCTCTTTCTGC 182 21 0 52.38 59.903 0.00 0.00 0.00 1.097 12 CCCTCATCCAGTGCTCTTTC 184 20 0 55.00 58.881 0.00 0.00 0.00 1.119 13 GATCCCTCATCCAGTGCTCTT 187 21 0 52.38 60.172 0.00 0.00 0.00 1.172 14 CCAGTGCTCTTTCTGCCAGT 177 20 0 55.00 61.175 0.00 0.00 0.00 1.175 15 TCATCCAGTGCTCTTTCTGC 181 20 0 50.00 58.810 0.00 0.00 0.00 1.190 16 TCCAGTGCTCTTTCTGCCA 178 19 0 52.63 59.785 0.00 0.00 0.00 1.215 17 ATCCCTCATCCAGTGCTCTTT 186 21 0 47.62 59.749 0.00 0.00 0.00 1.251 18 TCCCTCATCCAGTGCTCTTTC 185 21 0 52.38 60.380 0.00 0.00 0.00 1.380 19 CCCTCATCCAGTGCTCTTTCT 184 21 0 52.38 60.380 0.00 0.00 0.00 1.380 20 TTGGCCCTCTGGTTAACCG 151 19 0 57.89 60.547 14.57 9.97 39.80 1.547 21 CCAGTGCTCTTTCTGCCAG 177 19 0 57.89 59.350 0.00 0.00 0.00 1.650 22 CCTCATCCAGTGCTCTTTCTG 183 21 0 52.38 59.287 0.00 0.00 0.00 1.713 23 GTGCTCTTTCTGCCAGTGT 174 19 0 52.63 58.895 0.00 0.00 0.00 2.105 24 CCTCATCCAGTGCTCTTTCT 183 20 0 50.00 57.849 0.00 0.00 0.00 2.151 25 TGGCCCTCTGGTTAACCG 150 18 0 61.11 59.845 14.57 9.97 39.80 2.155 26 GGCCCTCTGGTTAACCGT 149 18 0 61.11 59.845 16.79 0.00 39.80 2.155 27 TCATCCAGTGCTCTTTCTGCC 181 21 0 52.38 61.269 0.00 0.00 0.00 2.269 28 ATCCAGTGCTCTTTCTGCCAG 179 21 0 52.38 61.272 0.00 0.00 0.00 2.272 29 GGCCCTCTGGTTAACCGTG 149 19 0 63.16 61.289 9.36 6.35 39.80 2.289 30 TTGGCCCTCTGGTTAACCGT 151 20 0 55.00 62.367 16.79 0.00 39.80 2.367 31 TGCTCTTTCTGCCAGTGTC 173 19 0 52.63 58.595 0.00 0.00 0.00 2.405 32 GCTCTTTCTGCCAGTGTCA 172 19 0 52.63 58.595 0.00 0.00 0.00 2.405 33 CAGTGCTCTTTCTGCCAGT 176 19 0 52.63 58.590 0.00 0.00 0.00 2.410 34 AGTGCTCTTTCTGCCAGTG 175 19 0 52.63 58.590 0.00 0.00 0.00 2.410 35 TCCCTCATCCAGTGCTCTT 185 19 0 52.63 58.535 0.00 0.00 0.00 2.465 36 AGTGCTCTTTCTGCCAGTGTC 175 21 0 52.38 61.466 0.00 0.00 0.00 2.466 37 CATCCAGTGCTCTTTCTGCCA 180 21 0 52.38 61.542 0.00 0.00 0.00 2.542 38 ATCCAGTGCTCTTTCTGCC 179 19 0 52.63 58.350 0.00 0.00 0.00 2.650 39 ATCCCTCATCCAGTGCTCT 186 19 0 52.63 58.293 0.00 0.00 0.00 2.707 40 GTGCTCTTTCTGCCAGTGTCA 174 21 0 52.38 61.733 0.00 0.00 0.00 2.733 41 TGCTCTTTCTGCCAGTGTCAC 173 21 0 52.38 61.733 0.00 0.00 0.00 2.733 42 CAGTGCTCTTTCTGCCAGTGT 176 21 0 52.38 61.737 0.00 0.00 0.00 2.737 43 GATCCCTCATCCAGTGCTCTTT 187 22 0 50.00 60.771 0.00 0.00 0.00 2.771 44 ATCCCTCATCCAGTGCTCTTTC 186 22 0 50.00 60.771 0.00 0.00 0.00 2.771 45 TGGCCCTCTGGTTAACCGT 150 19 0 57.89 61.787 16.79 0.00 39.80 2.787 46 GATCCCTCATCCAGTGCTC 187 19 0 57.89 57.905 0.00 0.00 0.00 3.095 47 CCCTCATCCAGTGCTCTTT 184 19 0 52.63 57.642 0.00 0.00 0.00 3.358 48 CCAGTGCTCTTTCTGCCAGTG 177 21 0 57.14 62.397 0.00 0.00 0.00 3.397 49 GCCCTCTGGTTAACCGTG 148 18 0 61.11 58.599 9.36 6.35 39.80 3.401 50 TCCAGTGCTCTTTCTGCCAGT 178 21 0 52.38 62.602 0.00 0.00 0.00 3.602 51 CCCTCATCCAGTGCTCTTTCTG 184 22 0 54.55 61.611 0.00 0.00 0.00 3.611 52 TCCCTCATCCAGTGCTCTTTCT 185 22 0 50.00 61.787 0.00 0.00 0.00 3.787 53 CATCCAGTGCTCTTTCTGC 180 19 0 52.63 57.204 0.00 0.00 0.00 3.796 54 CCAGTGCTCTTTCTGCCA 177 18 0 55.56 58.100 0.00 0.00 0.00 3.900 55 CCTCATCCAGTGCTCTTTCTGC 183 22 0 54.55 62.182 0.00 0.00 0.00 4.182 56 CTCATCCAGTGCTCTTTCTGCC 182 22 0 54.55 62.182 0.00 0.00 0.00 4.182 57 TCCAGTGCTCTTTCTGCC 178 18 0 55.56 57.779 0.00 0.00 0.00 4.221 58 TCCCTCATCCAGTGCTCT 185 18 0 55.56 57.701 0.00 0.00 0.00 4.299 59 CATCCAGTGCTCTTTCTGCCAG 180 22 0 54.55 62.439 0.00 0.00 0.00 4.439 60 CAGTGCTCTTTCTGCCAGTGTC 176 22 0 54.55 62.611 0.00 0.00 0.00 4.611 61 TTGGCCCTCTGGTTAACC 151 18 0 55.56 57.328 18.88 18.88 0.00 4.672 62 GATCCCTCATCCAGTGCTCTTTC 187 23 0 52.17 61.680 0.00 0.00 0.00 4.680 63 TGCTCTTTCTGCCAGTGT 173 18 0 50.00 57.275 0.00 0.00 0.00 4.725 64 GTGCTCTTTCTGCCAGTGTCAC 174 22 0 54.55 62.861 0.00 0.00 0.00 4.861 65 TCATCCAGTGCTCTTTCTGCCA 181 22 0 50.00 62.893 0.00 0.00 0.00 4.893 66 ATCCAGTGCTCTTTCTGCCAGT 179 22 0 50.00 62.901 0.00 0.00 0.00 4.901 67 ATCCCTCATCCAGTGCTCTTTCT 186 23 0 47.83 62.110 0.00 0.00 0.00 5.110 68 TCCCTCATCCAGTGCTCTTTCTG 185 23 0 52.17 62.900 0.00 0.00 0.00 5.900 69 GATCCCTCATCCAGTGCTCTTTCT 187 24 0 50.00 62.921 0.00 0.00 0.00 6.921 primer3-2.3.6/test/primer1_th_list_last/included_test_w_middle.int0000664000077300007730000001364312231156243024400 0ustar ioanaioanaACCEPTABLE INTERNAL OLIGOS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TGTTGTGTGTGTGTGTCTGTTGTGTAT 164 27 0 40.74 57.503 0.00 0.00 0.00 9.497 1 TGTTGTGTGTGTGTGTCTGTTGTGTA 164 26 0 42.31 57.356 0.00 0.00 0.00 8.644 2 GTGTTGTGTGTGTGTGTCTGTTGTGTA 163 27 0 44.44 58.211 0.00 0.00 0.00 8.789 3 TGTTGTGTGTGTGTGTCTGTTGTGT 164 25 0 44.00 57.701 0.00 0.00 0.00 7.299 4 GTGTTGTGTGTGTGTGTCTGTTGTGT 163 26 0 46.15 58.569 0.00 0.00 0.00 7.431 5 TGTGTTGTGTGTGTGTGTCTGTTGTGT 162 27 0 44.44 59.651 0.00 0.00 0.00 7.349 6 GTGTTGTGTGTGTGTGTCTGTTGTG 163 25 0 48.00 57.428 0.00 0.00 0.00 7.572 7 TGTGTTGTGTGTGTGTGTCTGTTGTG 162 26 0 46.15 58.570 0.00 0.00 0.00 7.430 8 GTGTGTTGTGTGTGTGTGTCTGTTGTG 161 27 0 48.15 59.357 0.00 0.00 0.00 7.643 9 TGTGTTGTGTGTGTGTGTCTGTTGT 162 25 0 44.00 57.701 0.00 0.00 0.00 7.299 10 GTGTGTTGTGTGTGTGTGTCTGTTGT 161 26 0 46.15 58.569 0.00 0.00 0.00 7.431 11 TGTGTGTTGTGTGTGTGTGTCTGTTGT 160 27 0 44.44 59.651 0.00 0.00 0.00 7.349 12 GTGTGTTGTGTGTGTGTGTCTGTTG 161 25 0 48.00 57.428 0.00 0.00 0.00 7.572 13 TGTGTGTTGTGTGTGTGTGTCTGTTG 160 26 0 46.15 58.570 0.00 0.00 0.00 7.430 14 CTGTGTGTTGTGTGTGTGTGTCTGTTG 159 27 0 48.15 59.151 0.00 0.00 0.00 7.849 15 TGTGTGTTGTGTGTGTGTGTCTGTT 160 25 0 44.00 57.701 0.00 0.00 0.00 7.299 16 CTGTGTGTTGTGTGTGTGTGTCTGTT 159 26 0 46.15 58.351 0.00 0.00 0.00 7.649 17 ACTGTGTGTTGTGTGTGTGTGTCTGTT 158 27 0 44.44 59.441 0.00 0.00 0.00 7.559 18 TGTGTGTTGTGTGTGTGTGTCTGT 160 24 0 45.83 57.366 0.00 0.00 0.00 6.634 19 CTGTGTGTTGTGTGTGTGTGTCTGT 159 25 0 48.00 58.058 0.00 0.00 0.00 6.942 20 ACTGTGTGTTGTGTGTGTGTGTCTGT 158 26 0 46.15 59.198 0.00 0.00 0.00 6.802 21 GACTGTGTGTTGTGTGTGTGTGTCTGT 157 27 0 48.15 59.759 0.00 0.00 0.00 7.241 22 ACTGTGTGTTGTGTGTGTGTGTCTG 158 25 0 48.00 58.058 0.00 0.00 0.00 6.942 23 GACTGTGTGTTGTGTGTGTGTGTCTG 157 26 0 50.00 58.689 0.00 0.00 0.00 7.311 24 TGACTGTGTGTTGTGTGTGTGTGTCTG 156 27 0 48.15 59.759 0.00 0.00 0.00 7.241 25 ACTGTGTGTTGTGTGTGTGTGTCT 158 24 0 45.83 57.123 0.00 0.00 0.00 6.877 26 GACTGTGTGTTGTGTGTGTGTGTCT 157 25 0 48.00 57.834 0.00 0.00 0.00 7.166 27 TGACTGTGTGTTGTGTGTGTGTGTCT 156 26 0 46.15 58.981 0.00 0.00 0.00 7.019 28 TTGACTGTGTGTTGTGTGTGTGTGTCT 155 27 0 44.44 59.232 0.00 0.00 0.00 7.768 29 TGACTGTGTGTTGTGTGTGTGTGTC 156 25 0 48.00 58.063 0.00 0.00 0.00 6.937 30 TTGACTGTGTGTTGTGTGTGTGTGTC 155 26 0 46.15 58.355 0.00 0.00 0.00 7.645 31 CTTGACTGTGTGTTGTGTGTGTGTGTC 154 27 0 48.15 58.946 0.00 0.00 0.00 8.054 32 TGACTGTGTGTTGTGTGTGTGTGT 156 24 0 45.83 57.366 0.00 0.00 0.00 6.634 33 TTGACTGTGTGTTGTGTGTGTGTGT 155 25 0 44.00 57.701 0.00 0.00 0.00 7.299 34 CTTGACTGTGTGTTGTGTGTGTGTGT 154 26 0 46.15 58.351 0.00 0.00 0.00 7.649 35 TCTTGACTGTGTGTTGTGTGTGTGTGT 153 27 0 44.44 59.232 0.00 0.00 0.00 7.768 36 CTTGACTGTGTGTTGTGTGTGTGTG 154 25 0 48.00 57.201 0.00 0.00 0.00 7.799 37 TCTTGACTGTGTGTTGTGTGTGTGTG 153 26 0 46.15 58.136 0.00 0.00 0.00 7.864 38 TTCTTGACTGTGTGTTGTGTGTGTGTG 152 27 0 44.44 58.415 0.00 0.00 0.00 8.585 39 TCTTGACTGTGTGTTGTGTGTGTGT 153 25 0 44.00 57.242 0.00 0.00 0.00 7.758 40 TTCTTGACTGTGTGTTGTGTGTGTGT 152 26 0 42.31 57.569 0.00 0.00 0.00 8.431 41 CTTCTTGACTGTGTGTTGTGTGTGTGT 151 27 0 44.44 58.203 0.00 0.00 0.00 8.797 42 CTTCTTGACTGTGTGTTGTGTGTGTG 151 26 0 46.15 57.089 0.00 0.00 0.00 8.911 43 GCTTCTTGACTGTGTGTTGTGTGTGTG 150 27 0 48.15 59.201 0.00 0.00 0.00 7.799 44 GCTTCTTGACTGTGTGTTGTGTGTGT 150 26 0 46.15 58.403 0.00 0.00 0.00 7.597 45 AGCTTCTTGACTGTGTGTTGTGTGTGT 149 27 0 44.44 59.282 0.00 0.00 0.00 7.718 46 GCTTCTTGACTGTGTGTTGTGTGTG 150 25 0 48.00 57.254 0.00 0.00 0.00 7.746 47 AGCTTCTTGACTGTGTGTTGTGTGTG 149 26 0 46.15 58.184 0.00 0.00 0.00 7.816 48 TAGCTTCTTGACTGTGTGTTGTGTGTG 148 27 0 44.44 57.838 0.00 0.00 0.00 9.162 49 AGCTTCTTGACTGTGTGTTGTGTGT 149 25 0 44.00 57.290 0.00 0.00 0.00 7.710 50 GTAGCTTCTTGACTGTGTGTTGTGTGT 147 27 0 44.44 57.837 0.00 0.00 0.00 9.163 51 TGTAGCTTCTTGACTGTGTGTTGTGTG 146 27 0 44.44 57.838 0.00 0.00 0.00 9.162 52 ATGTAGCTTCTTGACTGTGTGTTGTGT 145 27 0 40.74 57.120 0.00 0.00 0.00 9.880 53 GGATGTAGCTTCTTGACTGTGTGTTGT 143 27 0 44.44 57.452 0.00 0.00 0.00 9.548 54 TGGATGTAGCTTCTTGACTGTGTGTTG 142 27 0 44.44 57.454 0.00 0.00 0.00 9.546 55 GGGTGTGTCTGTTAAACATGTGTGGTA 89 27 0 44.44 57.386 0.00 0.00 0.00 9.614 56 GGGTGTGTCTGTTAAACATGTGTGGT 89 26 0 46.15 57.721 0.00 0.00 0.00 8.279 57 TGGGTGTGTCTGTTAAACATGTGTGGT 88 27 0 44.44 58.846 0.00 0.00 0.00 8.154 58 TGGGTGTGTCTGTTAAACATGTGTGG 88 26 0 46.15 57.722 0.00 0.00 0.00 8.278 59 GTGGGTGTGTCTGTTAAACATGTGTGG 87 27 0 48.15 58.564 0.00 0.00 0.00 8.436 60 TGTGGGTGTGTCTGTTAAACATGTGTG 86 27 0 44.44 58.031 0.00 0.00 0.00 8.969 61 TGTGGGTGTGTCTGTTAAACATGTGT 86 26 0 42.31 57.156 0.00 0.00 0.00 8.844 62 TTGTGGGTGTGTCTGTTAAACATGTGT 85 27 0 40.74 57.477 0.00 0.00 0.00 9.523 63 CTTGTGGGTGTGTCTGTTAAACATGTG 84 27 0 44.44 57.011 0.00 0.00 0.00 9.989 64 TCTTGTGGGTGTGTCTGTTAAACATGT 83 27 0 40.74 57.045 0.00 0.00 0.00 9.955 65 TGTCTTGTGGGTGTGTCTGTTAAACAT 81 27 0 40.74 57.045 0.00 0.00 0.00 9.955 66 GTGTCTTGTGGGTGTGTCTGTTAAACA 80 27 0 44.44 57.768 0.00 0.00 0.00 9.232 67 TGTGTCTTGTGGGTGTGTCTGTTAAAC 79 27 0 44.44 57.768 0.00 0.00 0.00 9.232 primer3-2.3.6/test/primer1_th_list_last/SP01-02.txt.for0000664000077300007730000000232012231156243021410 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 GCTGTTTCACACTTACGGAA 8 20 0 45.00 57.204 0.00 0.00 0.00 2.796 1 GCTGTTTCACACTTACGGAAA 8 21 0 42.86 57.949 0.00 0.00 0.00 3.051 2 GCTGTTTCACACTTACGGAAATG 8 23 0 43.48 59.687 0.00 0.00 0.00 3.313 3 TGTTTCACACTTACGGAAATGGA 10 23 0 39.13 59.482 0.00 0.00 0.00 3.518 4 GCTGTTTCACACTTACGGAAAT 8 22 0 40.91 58.429 0.00 0.00 0.00 3.571 5 CTTAGCGTGCGTGGGGTC 142 18 0 66.67 61.698 0.00 0.00 0.00 3.698 6 CTGTTTCACACTTACGGAAATGG 9 23 0 43.48 59.129 0.00 0.00 0.00 3.871 7 TGTTTCACACTTACGGAAATGG 10 22 0 40.91 58.094 0.00 0.00 0.00 3.906 8 GTTTCACACTTACGGAAATGGA 11 22 0 40.91 57.832 0.00 0.00 0.00 4.168 9 CTGTTTCACACTTACGGAAATGGA 9 24 0 41.67 60.417 0.00 0.00 0.00 4.417 10 GCTGTTTCACACTTACGGAAATGG 8 24 0 45.83 61.766 0.00 0.00 0.00 5.766 11 GCTGTTTCACACTTACGGAAATGGA 8 25 0 44.00 62.926 0.00 0.00 0.00 7.926 primer3-2.3.6/test/primer1_th_list_last/SP04-07.txt.rev0000664000077300007730000015215512231156243021442 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TGTGGCTAGTCATGGAGGTG 254 20 0 55.00 60.025 0.00 0.00 0.00 0.025 1 TCCCACAGCTGTAGGAAGTG 216 20 0 55.00 59.953 18.98 7.67 38.58 0.047 2 TCTCCTAGCTGGGTTTGTGG 269 20 0 55.00 59.951 11.17 0.00 36.53 0.049 3 GAGCCTCATTTTCCCACAGC 227 20 0 55.00 60.099 0.00 0.00 0.00 0.099 4 CTTCAGTGTGTACATGGCGG 115 20 0 55.00 59.820 0.00 0.00 0.00 0.180 5 CCCACAGCTGTAGGAAGTGT 215 20 0 55.00 60.242 27.09 1.39 0.00 0.242 6 TGCCCTGAAGAGCCTCATTT 236 20 0 50.00 60.243 0.00 0.00 0.00 0.243 7 GCAGTTACACCACCCCATTC 299 20 0 55.00 59.742 0.00 0.00 0.00 0.258 8 CCTAGCTGGGTTTGTGGCTA 266 20 0 55.00 60.315 1.38 0.00 36.85 0.315 9 ATTCTTTCCGTGGCTCTCCT 283 20 0 50.00 59.658 0.00 0.00 0.00 0.342 10 GCCTCATTTTCCCACAGCTG 225 20 0 55.00 60.383 13.98 13.98 0.00 0.383 11 CTTCAGTGCATTCCCTTGGC 153 20 0 55.00 60.383 0.00 0.00 0.00 0.383 12 TAGCTGGGTTTGTGGCTAGT 264 20 0 50.00 59.583 0.00 0.00 36.85 0.417 13 AGGGAAGGTCTTCAGTGCAT 162 20 0 50.00 59.579 12.62 0.00 41.71 0.421 14 AGGTCTTCAGTGCATTCCCT 157 20 0 50.00 59.579 0.00 0.00 0.00 0.421 15 CTAGTCATGGAGGTGCCCTG 249 20 0 60.00 60.458 0.00 0.00 32.62 0.458 16 TGCAGTTACACCACCCCATT 300 20 0 50.00 60.464 0.00 0.00 0.00 0.464 17 CCATTCTTTCCGTGGCTCTC 285 20 0 55.00 59.533 0.00 0.00 0.00 0.467 18 CATTCTTTCCGTGGCTCTCC 284 20 0 55.00 59.533 0.00 0.00 0.00 0.467 19 AGCTGTAGGAAGTGTGTGGT 210 20 0 50.00 59.512 0.00 0.00 0.00 0.488 20 AGCCTCATTTTCCCACAGCT 226 20 0 50.00 60.537 0.00 0.00 0.00 0.537 21 GGGTTTGTGGCTAGTCATGG 259 20 0 55.00 59.454 0.00 0.00 0.00 0.546 22 TCTTTCCGTGGCTCTCCTAG 281 20 0 55.00 59.449 0.00 0.00 0.00 0.551 23 TTGTGGCTAGTCATGGAGGT 255 20 0 50.00 59.286 0.00 0.00 0.00 0.714 24 GCATTCCCTTGGCCACATTC 146 20 0 55.00 60.741 0.00 0.00 0.00 0.741 25 TTCCCACAGCTGTAGGAAGT 217 20 0 50.00 59.213 23.38 0.00 46.50 0.787 26 CCCTTGGCCACATTCTTAGG 141 20 0 55.00 59.158 10.84 10.84 0.00 0.842 27 CCTTGGCCACATTCTTAGGG 140 20 0 55.00 59.158 10.05 0.00 0.00 0.842 28 AGCTGGGTTTGTGGCTAGTC 263 20 0 55.00 60.892 0.00 0.00 33.50 0.892 29 CCACAGCTGTAGGAAGTGTG 214 20 0 55.00 59.107 23.93 9.86 33.62 0.893 30 CAGCTGTAGGAAGTGTGTGG 211 20 0 55.00 59.107 5.76 0.00 0.00 0.893 31 TAGTCATGGAGGTGCCCTGA 248 20 0 55.00 60.910 0.00 0.00 39.44 0.910 32 GCCCTGAAGAGCCTCATTTT 235 20 0 50.00 59.083 0.00 0.00 0.00 0.917 33 CATTCCCTTGGCCACATTCT 145 20 0 50.00 59.076 0.00 0.00 0.00 0.924 34 CCCATTCTTTCCGTGGCTCT 286 20 0 55.00 60.964 0.00 0.00 0.00 0.964 35 GTGCCCTGAAGAGCCTCATT 237 20 0 55.00 60.966 0.00 0.00 0.00 0.966 36 CCTCATTTTCCCACAGCTGT 224 20 0 50.00 59.011 19.87 18.06 0.00 0.989 37 TTCTTTCCGTGGCTCTCCTA 282 20 0 50.00 59.001 0.00 0.00 0.00 0.999 38 TGGCTAGTCATGGAGGTGC 252 19 0 57.89 60.016 0.00 0.00 0.00 1.016 39 CATGGAGGTGCCCTGAAGAG 244 20 0 60.00 61.033 0.00 0.00 35.27 1.033 40 TTTGTGGCTAGTCATGGAGGT 256 21 0 47.62 59.962 0.00 0.00 0.00 1.038 41 TCATGGAGGTGCCCTGAAG 245 19 0 57.89 59.938 0.00 0.00 39.11 1.062 42 CATGGAGGTGCCCTGAAGA 244 19 0 57.89 59.938 0.00 0.00 35.27 1.062 43 TAGGGAAGGTCTTCAGTGCA 163 20 0 50.00 58.918 12.62 0.00 41.71 1.082 44 TTCTTTCCGTGGCTCTCCTAG 282 21 0 52.38 60.103 0.00 0.00 0.00 1.103 45 GGTCTTCAGTGCATTCCCTTG 156 21 0 52.38 60.104 0.00 0.00 0.00 1.104 46 GTCTTCAGTGCATTCCCTTGG 155 21 0 52.38 60.104 0.00 0.00 0.00 1.104 47 CTAGCTGGGTTTGTGGCTAG 265 20 0 55.00 58.884 7.07 7.07 45.49 1.116 48 CTGGGTTTGTGGCTAGTCATG 261 21 0 52.38 59.832 0.00 0.00 0.00 1.168 49 GGGAAGGTCTTCAGTGCATTC 161 21 0 52.38 59.832 12.62 0.00 41.71 1.168 50 GGAAGGTCTTCAGTGCATTCC 160 21 0 52.38 59.832 12.62 4.02 41.71 1.168 51 GAAGGTCTTCAGTGCATTCCC 159 21 0 52.38 59.832 6.42 0.00 39.78 1.168 52 GCCCTGAAGAGCCTCATTTTC 235 21 0 52.38 60.172 0.00 0.00 0.00 1.172 53 TCTTCAGTGTGTACATGGCG 116 20 0 50.00 58.826 0.00 0.00 0.00 1.174 54 GCTGGGTTTGTGGCTAGTCA 262 20 0 55.00 61.177 0.00 0.00 0.00 1.177 55 ACACCACCCCATTCTTTCCG 293 20 0 55.00 61.183 0.00 0.00 0.00 1.183 56 CACCACCCCATTCTTTCCGT 292 20 0 55.00 61.183 0.00 0.00 0.00 1.183 57 ACCACCCCATTCTTTCCGTG 291 20 0 55.00 61.183 0.00 0.00 0.00 1.183 58 AGCTGGGTTTGTGGCTAGT 263 19 0 52.63 59.782 0.00 0.00 33.50 1.218 59 TGCAGTTACACCACCCCAT 300 19 0 52.63 59.780 0.00 0.00 0.00 1.220 60 ACCACCCCATTCTTTCCGT 291 19 0 52.63 59.780 0.00 0.00 0.00 1.220 61 CACAGCTGTAGGAAGTGTGTG 213 21 0 52.38 59.769 13.96 0.00 32.54 1.231 62 AGGGAAGGTCTTCAGTGCATT 162 21 0 47.62 60.242 12.62 0.00 41.71 1.242 63 AAGGTCTTCAGTGCATTCCCT 158 21 0 47.62 60.242 0.00 0.00 0.00 1.242 64 AGGTCTTCAGTGCATTCCCTT 157 21 0 47.62 60.242 0.00 0.00 0.00 1.242 65 TGGCCACATTCTTAGGGAACA 137 21 0 47.62 60.242 7.04 0.00 37.28 1.242 66 GTCATGGAGGTGCCCTGAA 246 19 0 57.89 60.244 0.00 0.00 42.92 1.244 67 CATTCCCTTGGCCACATTCTT 145 21 0 47.62 59.755 0.00 0.00 0.00 1.245 68 TGGGTTTGTGGCTAGTCATG 260 20 0 50.00 58.725 0.00 0.00 0.00 1.275 69 TCTTCAGTGCATTCCCTTGG 154 20 0 50.00 58.721 0.00 0.00 0.00 1.279 70 GGGAAGGTCTTCAGTGCATT 161 20 0 50.00 58.720 12.62 0.00 41.71 1.280 71 AAGGTCTTCAGTGCATTCCC 158 20 0 50.00 58.720 0.00 0.00 0.00 1.280 72 GGTCTTCAGTGCATTCCCTT 156 20 0 50.00 58.720 0.00 0.00 0.00 1.280 73 GTCATGGAGGTGCCCTGAAG 246 20 0 60.00 61.317 0.00 0.00 42.92 1.317 74 GTGCCCTGAAGAGCCTCAT 237 19 0 57.89 60.320 0.00 0.00 0.00 1.320 75 GCATTCCCTTGGCCACATT 146 19 0 52.63 59.635 0.00 0.00 0.00 1.365 76 ATGGAGGTGCCCTGAAGAG 243 19 0 57.89 59.629 0.00 0.00 32.62 1.371 77 GTGGCTAGTCATGGAGGTGC 253 20 0 60.00 61.379 0.00 0.00 0.00 1.379 78 TTAGGGAAGGTCTTCAGTGCA 164 21 0 47.62 59.611 12.62 0.00 41.71 1.389 79 GGTTTGTGGCTAGTCATGGAG 258 21 0 52.38 59.559 0.00 0.00 0.00 1.441 80 GTTTGTGGCTAGTCATGGAGG 257 21 0 52.38 59.559 0.00 0.00 0.00 1.441 81 CCCTGAAGAGCCTCATTTTCC 234 21 0 52.38 59.554 0.00 0.00 0.00 1.446 82 CCTGAAGAGCCTCATTTTCCC 233 21 0 52.38 59.554 0.00 0.00 0.00 1.446 83 TGCCCTGAAGAGCCTCATT 236 19 0 52.63 59.548 0.00 0.00 0.00 1.452 84 CTCTCCTAGCTGGGTTTGTG 270 20 0 55.00 58.522 11.17 2.62 36.53 1.478 85 TGCATTCCCTTGGCCACATT 147 20 0 50.00 61.491 0.00 0.00 0.00 1.491 86 TCATGGAGGTGCCCTGAAGA 245 20 0 55.00 61.500 0.00 0.00 39.11 1.500 87 ATTCTTTCCGTGGCTCTCCTA 283 21 0 47.62 59.474 0.00 0.00 0.00 1.526 88 TCCTAGCTGGGTTTGTGGC 267 19 0 57.89 60.548 9.65 0.00 36.53 1.548 89 CCTAGCTGGGTTTGTGGCT 266 19 0 57.89 60.549 1.38 0.00 38.85 1.549 90 GGTTTGTGGCTAGTCATGGA 258 20 0 50.00 58.434 0.00 0.00 0.00 1.566 91 CTGGGTTTGTGGCTAGTCAT 261 20 0 50.00 58.429 0.00 0.00 0.00 1.571 92 AGAGCCTCATTTTCCCACAG 228 20 0 50.00 58.425 0.00 0.00 0.00 1.575 93 CCCATTCTTTCCGTGGCTC 286 19 0 57.89 59.422 0.00 0.00 0.00 1.578 94 TGGCCACATTCTTAGGGAAC 137 20 0 50.00 58.422 7.04 0.00 37.28 1.578 95 GGCCACATTCTTAGGGAACA 136 20 0 50.00 58.422 0.00 0.00 37.28 1.578 96 TTCCCACAGCTGTAGGAAGTG 217 21 0 52.38 60.586 23.38 10.07 46.50 1.586 97 CTCCTAGCTGGGTTTGTGGC 268 20 0 60.00 61.600 7.71 0.00 36.53 1.600 98 TAGGGAAGGTCTTCAGTGCAT 163 21 0 47.62 59.398 12.62 0.00 41.71 1.602 99 CACAGCTGTAGGAAGTGTGT 213 20 0 50.00 58.381 13.96 0.00 31.50 1.619 100 ACAGCTGTAGGAAGTGTGTG 212 20 0 50.00 58.381 20.35 0.00 0.00 1.619 101 GCTGGGTTTGTGGCTAGTC 262 19 0 57.89 59.348 0.00 0.00 0.00 1.652 102 TAGCTGGGTTTGTGGCTAGTC 264 21 0 52.38 60.654 0.00 0.00 36.85 1.654 103 TTGTGGCTAGTCATGGAGGTG 255 21 0 52.38 60.655 0.00 0.00 0.00 1.655 104 CTAGCTGGGTTTGTGGCTAGT 265 21 0 52.38 60.656 11.85 0.00 45.75 1.656 105 CACCACCCCATTCTTTCCG 292 19 0 57.89 59.343 0.00 0.00 0.00 1.657 106 CCACCCCATTCTTTCCGTG 290 19 0 57.89 59.343 0.00 0.00 0.00 1.657 107 CACCCCATTCTTTCCGTGG 289 19 0 57.89 59.343 0.00 0.00 0.00 1.657 108 TCCCTTGGCCACATTCTTAGG 142 21 0 52.38 60.661 0.00 0.00 0.00 1.661 109 CCTTGGCCACATTCTTAGGGA 140 21 0 52.38 60.661 10.05 0.00 0.00 1.661 110 AGCTGTAGGAAGTGTGTGGTA 210 21 0 47.62 59.335 0.00 0.00 0.00 1.665 111 TAGTCATGGAGGTGCCCTG 248 19 0 57.89 59.323 0.00 0.00 32.62 1.677 112 ATTCCCTTGGCCACATTCTT 144 20 0 45.00 58.317 0.00 0.00 0.00 1.683 113 ATCTTCAGTGTGTACATGGCG 117 21 0 47.62 59.292 0.00 0.00 0.00 1.708 114 TTCAGTGCATTCCCTTGGC 152 19 0 52.63 59.260 0.00 0.00 0.00 1.740 115 GGCTAGTCATGGAGGTGCC 251 19 0 63.16 60.770 3.48 3.48 37.10 1.770 116 GCTAGTCATGGAGGTGCCC 250 19 0 63.16 60.770 0.00 0.00 35.38 1.770 117 GCTGTAGGAAGTGTGTGGTAC 209 21 0 52.38 59.226 0.00 0.00 0.00 1.774 118 AGTCATGGAGGTGCCCTGAA 247 20 0 55.00 61.795 0.00 0.00 42.92 1.795 119 TTCTTTCCGTGGCTCTCCT 282 19 0 52.63 59.170 0.00 0.00 0.00 1.830 120 TTCAGTGCATTCCCTTGGCC 152 20 0 55.00 61.832 0.00 0.00 0.00 1.832 121 CCACAGCTGTAGGAAGTGTGT 214 21 0 52.38 60.858 23.93 0.00 32.90 1.858 122 ACAGCTGTAGGAAGTGTGTGG 212 21 0 52.38 60.858 20.35 0.00 0.00 1.858 123 CAGCTGTAGGAAGTGTGTGGT 211 21 0 52.38 60.858 5.76 0.00 0.00 1.858 124 TTTGTGGCTAGTCATGGAGG 256 20 0 50.00 58.138 0.00 0.00 0.00 1.862 125 TGCATTCCCTTGGCCACAT 147 19 0 52.63 60.863 0.00 0.00 0.00 1.863 126 TGAAGAGCCTCATTTTCCCAC 231 21 0 47.62 59.136 0.00 0.00 0.00 1.864 127 GAAGAGCCTCATTTTCCCACA 230 21 0 47.62 59.136 0.00 0.00 0.00 1.864 128 TTCAGTGTGTACATGGCGGC 114 20 0 55.00 61.864 0.27 0.27 0.00 1.864 129 AAGAGCCTCATTTTCCCACAG 229 21 0 47.62 59.133 0.00 0.00 0.00 1.867 130 TTGGCCACATTCTTAGGGAAC 138 21 0 47.62 59.132 0.00 0.00 37.28 1.868 131 TGCCCTGAAGAGCCTCATTTT 236 21 0 47.62 60.872 0.00 0.00 0.00 1.872 132 TCCCTTGGCCACATTCTTAG 142 20 0 50.00 58.125 0.00 0.00 0.00 1.875 133 CTTGGCCACATTCTTAGGGA 139 20 0 50.00 58.125 0.00 0.00 0.00 1.875 134 CCACCCCATTCTTTCCGTGG 290 20 0 60.00 61.890 0.00 0.00 0.00 1.890 135 AGGGAAGGTCTTCAGTGCA 162 19 0 52.63 59.084 12.62 0.00 41.71 1.916 136 CCCCATTCTTTCCGTGGCT 287 19 0 57.89 60.929 0.00 0.00 0.00 1.929 137 TACACCACCCCATTCTTTCCG 294 21 0 52.38 60.932 0.00 0.00 0.00 1.932 138 GGGTTTGTGGCTAGTCATGGA 259 21 0 52.38 60.934 0.00 0.00 0.00 1.934 139 CAGTTACACCACCCCATTCT 298 20 0 50.00 58.052 0.00 0.00 0.00 1.948 140 CCCCATTCTTTCCGTGGCTC 287 20 0 60.00 61.952 0.00 0.00 0.00 1.952 141 TACACCACCCCATTCTTTCC 294 20 0 50.00 58.044 0.00 0.00 0.00 1.956 142 CTAGTCATGGAGGTGCCCT 249 19 0 57.89 59.012 0.00 0.00 36.47 1.988 143 CATTCTTTCCGTGGCTCTCCT 284 21 0 52.38 60.996 0.00 0.00 0.00 1.996 144 CTCTCCTAGCTGGGTTTGTGG 270 21 0 57.14 60.996 3.50 0.00 36.53 1.996 145 GGCCACATTCTTAGGGAACATC 136 22 0 50.00 59.977 0.00 0.00 37.28 2.023 146 AAGAGCCTCATTTTCCCACA 229 20 0 45.00 57.961 0.00 0.00 0.00 2.039 147 ATTAGGGAAGGTCTTCAGTGCA 165 22 0 45.45 60.039 12.62 0.00 41.71 2.039 148 TTAGGGAAGGTCTTCAGTGCAT 164 22 0 45.45 60.039 12.62 0.00 41.71 2.039 149 TAGGGAAGGTCTTCAGTGCATT 163 22 0 45.45 60.039 12.62 0.00 41.71 2.039 150 AGCCTCATTTTCCCACAGC 226 19 0 52.63 58.953 0.00 0.00 0.00 2.047 151 GCCTCATTTTCCCACAGCT 225 19 0 52.63 58.953 0.00 0.00 0.00 2.047 152 GTGCATTCCCTTGGCCAC 148 18 0 61.11 59.930 5.88 0.00 0.00 2.070 153 GGCCACATTCTTAGGGAACAT 136 21 0 47.62 58.919 0.00 0.00 37.28 2.081 154 AGTGCATTCCCTTGGCCA 149 18 0 55.56 60.083 7.04 3.53 0.00 2.083 155 TCCTAGCTGGGTTTGTGGCT 267 20 0 55.00 62.085 9.65 0.00 38.85 2.085 156 GGTACACCCACAATGTCTTAGC 193 22 0 50.00 59.914 0.00 0.00 0.00 2.086 157 CCTCATTTTCCCACAGCTGTAG 224 22 0 50.00 59.913 14.35 9.61 0.00 2.087 158 CTCATTTTCCCACAGCTGTAGG 223 22 0 50.00 59.913 23.28 23.28 0.00 2.087 159 GTTACACCACCCCATTCTTTCC 296 22 0 50.00 60.108 0.00 0.00 0.00 2.108 160 CCTCATTTTCCCACAGCTGTA 224 21 0 47.62 58.859 20.84 2.94 0.00 2.141 161 TCATTTTCCCACAGCTGTAGG 222 21 0 47.62 58.859 23.28 23.28 0.00 2.141 162 CATTTTCCCACAGCTGTAGGA 221 21 0 47.62 58.859 29.48 28.23 39.52 2.141 163 CTGAAGAGCCTCATTTTCCCA 232 21 0 47.62 58.855 0.00 0.00 0.00 2.145 164 TTCCCTTGGCCACATTCTTAG 143 21 0 47.62 58.850 0.00 0.00 0.00 2.150 165 CTTGGCCACATTCTTAGGGAA 139 21 0 47.62 58.850 0.00 0.00 38.69 2.150 166 GTGCCCTGAAGAGCCTCA 237 18 0 61.11 59.845 0.00 0.00 0.00 2.155 167 CTTGGCCACATTCTTAGGGAAC 139 22 0 50.00 60.174 0.00 0.00 37.28 2.174 168 CTGAAGAGCCTCATTTTCCCAC 232 22 0 50.00 60.174 0.00 0.00 0.00 2.174 169 GAAGAGCCTCATTTTCCCACAG 230 22 0 50.00 60.174 0.00 0.00 0.00 2.174 170 AGTCATGGAGGTGCCCTGA 247 19 0 57.89 61.179 0.00 0.00 39.44 2.179 171 GTGCATTCCCTTGGCCACAT 148 20 0 55.00 62.193 0.00 0.00 0.00 2.193 172 GCTGTAGGAAGTGTGTGGTA 209 20 0 50.00 57.795 0.00 0.00 0.00 2.205 173 TGGGTTTGTGGCTAGTCATGG 260 21 0 52.38 61.210 0.00 0.00 0.00 2.210 174 GCAGTTACACCACCCCATTCT 299 21 0 52.38 61.210 0.00 0.00 0.00 2.210 175 CAGTTACACCACCCCATTCTT 298 21 0 47.62 58.781 0.00 0.00 0.00 2.219 176 TTACACCACCCCATTCTTTCC 295 21 0 47.62 58.776 0.00 0.00 0.00 2.224 177 TTAGGGAAGGTCTTCAGTGC 164 20 0 50.00 57.774 12.62 0.00 41.71 2.226 178 ACCCCATTCTTTCCGTGGC 288 19 0 57.89 61.230 0.00 0.00 0.00 2.230 179 TCAGTGCATTCCCTTGGCC 151 19 0 57.89 61.232 0.00 0.00 0.00 2.232 180 TCTTCAGTGTGTACATGGCGG 116 21 0 52.38 61.257 0.00 0.00 0.00 2.257 181 TCAGTGTGTACATGGCGGC 113 19 0 57.89 61.281 0.27 0.27 0.00 2.281 182 GCTAGTCATGGAGGTGCCCT 250 20 0 60.00 62.282 0.00 0.00 36.47 2.282 183 CAGTGTGTACATGGCGGC 112 18 0 61.11 59.705 0.27 0.27 0.00 2.295 184 TCATTTTCCCACAGCTGTAGGA 222 22 0 45.45 60.308 29.48 28.23 39.52 2.308 185 ACACCCACAATGTCTTAGCT 190 20 0 45.00 57.680 0.00 0.00 0.00 2.320 186 TTCAGTGTGTACATGGCGG 114 19 0 52.63 58.677 0.00 0.00 0.00 2.323 187 TGAAGAGCCTCATTTTCCCA 231 20 0 45.00 57.665 0.00 0.00 0.00 2.335 188 TTCCCTTGGCCACATTCTTA 143 20 0 45.00 57.655 0.00 0.00 0.00 2.345 189 TTGGCCACATTCTTAGGGAA 138 20 0 45.00 57.655 0.00 0.00 38.69 2.345 190 GTCTTCAGTGCATTCCCTTG 155 20 0 50.00 57.614 0.00 0.00 0.00 2.386 191 CAGTGCATTCCCTTGGCC 150 18 0 61.11 59.613 0.00 0.00 0.00 2.387 192 TGGTACACCCACAATGTCTT 194 20 0 45.00 57.599 0.00 0.00 39.49 2.401 193 TGCATTCCCTTGGCCACA 147 18 0 55.56 60.409 0.00 0.00 0.00 2.409 194 ACATCTTCAGTGTGTACATGGC 119 22 0 45.45 59.587 0.00 0.00 0.00 2.413 195 CATTCCCTTGGCCACATTCTTA 145 22 0 45.45 59.576 0.00 0.00 0.00 2.424 196 GCAGTTACACCACCCCATT 299 19 0 52.63 58.566 0.00 0.00 0.00 2.434 197 TGTGGCTAGTCATGGAGGT 254 19 0 52.63 58.539 0.00 0.00 0.00 2.461 198 GTCATGGAGGTGCCCTGA 246 18 0 61.11 59.520 0.00 0.00 39.44 2.480 199 AGTCATGGAGGTGCCCTG 247 18 0 61.11 59.518 0.00 0.00 32.62 2.482 200 TGCAGTTACACCACCCCATTC 300 21 0 52.38 61.483 0.00 0.00 0.00 2.483 201 ATTCTTTCCGTGGCTCTCCTAG 283 22 0 50.00 60.503 0.00 0.00 0.00 2.503 202 TAGTCATGGAGGTGCCCTGAA 248 21 0 52.38 61.511 0.00 0.00 42.92 2.511 203 CACCCCATTCTTTCCGTGGC 289 20 0 60.00 62.513 0.00 0.00 0.00 2.513 204 CCCACAGCTGTAGGAAGTGTG 215 21 0 57.14 61.531 27.09 12.86 33.62 2.531 205 CAGTGCATTCCCTTGGCCA 150 19 0 57.89 61.535 7.04 3.53 0.00 2.535 206 AGTGCATTCCCTTGGCCAC 149 19 0 57.89 61.536 5.88 0.00 0.00 2.536 207 TCCCACAGCTGTAGGAAGT 216 19 0 52.63 58.462 18.98 0.00 38.58 2.538 208 GCTGGGTTTGTGGCTAGTCAT 262 21 0 52.38 61.548 0.00 0.00 0.00 2.548 209 GTGCCCTGAAGAGCCTCATTT 237 21 0 52.38 61.551 0.00 0.00 0.00 2.551 210 AGAGCCTCATTTTCCCACAGC 228 21 0 52.38 61.551 0.00 0.00 0.00 2.551 211 GAGCCTCATTTTCCCACAGCT 227 21 0 52.38 61.551 0.00 0.00 0.00 2.551 212 CAGTTACACCACCCCATTCTTT 298 22 0 45.45 59.444 0.00 0.00 0.00 2.556 213 CATCTTCAGTGTGTACATGGCG 118 22 0 50.00 60.557 0.00 0.00 0.00 2.557 214 TGGCTAGTCATGGAGGTGCC 252 20 0 60.00 62.565 10.08 10.08 37.10 2.565 215 ACACCCACAATGTCTTAGCTT 190 21 0 42.86 58.429 0.00 0.00 0.00 2.571 216 CCCTTGGCCACATTCTTAGGG 141 21 0 57.14 61.626 20.63 20.63 0.00 2.626 217 ATTTTCCCACAGCTGTAGGA 220 20 0 45.00 57.367 29.48 28.23 39.52 2.633 218 CCCACAGCTGTAGGAAGTG 215 19 0 57.89 58.367 27.09 13.67 0.00 2.633 219 CAGCTGTAGGAAGTGTGTGGTA 211 22 0 50.00 60.633 5.76 0.00 0.00 2.633 220 AGCTGTAGGAAGTGTGTGGTAC 210 22 0 50.00 60.633 0.00 0.00 0.00 2.633 221 GGAGGTGCCCTGAAGAGC 241 18 0 66.67 60.641 0.00 0.00 32.62 2.641 222 GAGGTGCCCTGAAGAGCC 240 18 0 66.67 60.641 0.00 0.00 32.62 2.641 223 GGTGCCCTGAAGAGCCTC 238 18 0 66.67 60.641 0.00 0.00 0.00 2.641 224 TGGCCACATTCTTAGGGAACAT 137 22 0 45.45 60.645 7.04 0.00 37.28 2.645 225 CCATTCTTTCCGTGGCTCT 285 19 0 52.63 58.355 0.00 0.00 0.00 2.645 226 CTCCTAGCTGGGTTTGTGG 268 19 0 57.89 58.355 7.71 0.00 36.53 2.645 227 GCCCTGAAGAGCCTCATTT 235 19 0 52.63 58.337 0.00 0.00 0.00 2.663 228 GGAAGGTCTTCAGTGCATTC 160 20 0 50.00 57.327 12.62 0.00 41.71 2.673 229 GAAGGTCTTCAGTGCATTCC 159 20 0 50.00 57.327 6.42 0.00 39.78 2.673 230 CATTTTCCCACAGCTGTAGG 221 20 0 50.00 57.311 23.28 23.28 0.00 2.689 231 GAAGAGCCTCATTTTCCCAC 230 20 0 50.00 57.309 0.00 0.00 0.00 2.691 232 ATTCCCTTGGCCACATTCTTAG 144 22 0 45.45 59.308 0.00 0.00 0.00 2.692 233 AGGGAACATCTTCAGTGTGT 124 20 0 45.00 57.306 0.00 0.00 0.00 2.694 234 CCCCATTCTTTCCGTGGC 287 18 0 61.11 59.299 0.00 0.00 0.00 2.701 235 ATTAGGGAAGGTCTTCAGTGC 165 21 0 47.62 58.297 12.62 0.00 41.71 2.703 236 TCCCACAGCTGTAGGAAGTGT 216 21 0 52.38 61.711 18.98 0.00 38.58 2.711 237 AGTTACACCACCCCATTCTT 297 20 0 45.00 57.275 0.00 0.00 0.00 2.725 238 TGCAGTTACACCACCCCA 300 18 0 55.56 59.270 0.00 0.00 0.00 2.730 239 ACCCCATTCTTTCCGTGGCT 288 20 0 55.00 62.739 0.00 0.00 0.00 2.739 240 TCTTTCCGTGGCTCTCCTA 281 19 0 52.63 58.246 0.00 0.00 0.00 2.754 241 CATTCTTTCCGTGGCTCTCCTA 284 22 0 50.00 60.765 0.00 0.00 0.00 2.765 242 TCCTAGCTGGGTTTGTGGCTA 267 21 0 52.38 61.788 9.65 0.00 36.85 2.788 243 CAGTGCATTCCCTTGGCCAC 150 20 0 60.00 62.801 5.88 0.00 0.00 2.801 244 ATTCCCTTGGCCACATTCTTA 144 21 0 42.86 58.193 0.00 0.00 0.00 2.807 245 TCTTCAGTGCATTCCCTTGGC 154 21 0 52.38 61.821 0.00 0.00 0.00 2.821 246 AGTTACACCACCCCATTCTTTC 297 22 0 45.45 59.177 0.00 0.00 0.00 2.823 247 AGCCTCATTTTCCCACAGCTG 226 21 0 52.38 61.825 13.98 13.98 0.00 2.825 248 GTGCATTCCCTTGGCCACA 148 19 0 57.89 61.833 0.00 0.00 0.00 2.833 249 ACACCACCCCATTCTTTCC 293 19 0 52.63 58.165 0.00 0.00 0.00 2.835 250 TGAAGAGCCTCATTTTCCCACA 231 22 0 45.45 60.842 0.00 0.00 0.00 2.842 251 AAGGTCTTCAGTGCATTCCCTT 158 22 0 45.45 60.844 0.00 0.00 0.00 2.844 252 TTGGCCACATTCTTAGGGAACA 138 22 0 45.45 60.844 0.00 0.00 37.28 2.844 253 GTGGCTAGTCATGGAGGTG 253 19 0 57.89 58.140 0.00 0.00 0.00 2.860 254 ATGGAGGTGCCCTGAAGAGC 243 20 0 60.00 62.861 0.00 0.00 32.62 2.861 255 GGTGCCCTGAAGAGCCTCAT 238 20 0 60.00 62.861 0.00 0.00 0.00 2.861 256 TGGAGGTGCCCTGAAGAG 242 18 0 61.11 59.114 0.00 0.00 32.62 2.886 257 ACACCCACAATGTCTTAGCTTT 190 22 0 40.91 59.109 0.00 0.00 0.00 2.891 258 GCTGTAGGAAGTGTGTGGTACA 209 22 0 50.00 60.894 0.00 0.00 37.17 2.894 259 CTAGTCATGGAGGTGCCCTGA 249 21 0 57.14 61.913 0.00 0.00 39.44 2.913 260 CCCATTCTTTCCGTGGCTCTC 286 21 0 57.14 61.935 0.00 0.00 0.00 2.935 261 CCATTCTTTCCGTGGCTCTCC 285 21 0 57.14 61.935 0.00 0.00 0.00 2.935 262 AGTTACACCACCCCATTCTTT 297 21 0 42.86 58.053 0.00 0.00 0.00 2.947 263 ATTCTTTCCGTGGCTCTCC 283 19 0 52.63 58.051 0.00 0.00 0.00 2.949 264 GCCACATTCTTAGGGAACATCT 135 22 0 45.45 59.047 0.00 0.00 37.28 2.953 265 GTTTGTGGCTAGTCATGGAG 257 20 0 50.00 57.042 0.00 0.00 0.00 2.958 266 CCCTGAAGAGCCTCATTTTC 234 20 0 50.00 57.013 0.00 0.00 0.00 2.987 267 CCTGAAGAGCCTCATTTTCC 233 20 0 50.00 57.013 0.00 0.00 0.00 2.987 268 CTGAAGAGCCTCATTTTCCC 232 20 0 50.00 57.013 0.00 0.00 0.00 2.987 269 GTACACCCACAATGTCTTAGCT 192 22 0 45.45 58.991 0.00 0.00 0.00 3.009 270 GGGAAGGTCTTCAGTGCAT 161 19 0 52.63 57.956 12.62 0.00 41.71 3.044 271 AGGTCTTCAGTGCATTCCC 157 19 0 52.63 57.956 0.00 0.00 0.00 3.044 272 GGTCTTCAGTGCATTCCCT 156 19 0 52.63 57.956 0.00 0.00 0.00 3.044 273 GGGAACATCTTCAGTGTGTACA 123 22 0 45.45 58.926 0.00 0.00 0.00 3.074 274 GCCTCATTTTCCCACAGCTGT 225 21 0 52.38 62.095 19.87 18.06 0.00 3.095 275 GCTGTAGGAAGTGTGTGGT 209 19 0 52.63 57.902 0.00 0.00 0.00 3.098 276 CTGTAGGAAGTGTGTGGTACA 208 21 0 47.62 57.902 0.00 0.00 37.17 3.098 277 CCTAGCTGGGTTTGTGGC 266 18 0 61.11 58.901 1.38 0.00 0.00 3.099 278 CTGGGTTTGTGGCTAGTCA 261 19 0 52.63 57.888 0.00 0.00 0.00 3.112 279 CATCTTCAGTGTGTACATGGC 118 21 0 47.62 57.881 0.00 0.00 0.00 3.119 280 GCATTCCCTTGGCCACAT 146 18 0 55.56 58.880 0.00 0.00 0.00 3.120 281 TATTAGGGAAGGTCTTCAGTGCA 166 23 0 43.48 59.854 0.32 0.00 41.71 3.146 282 CTTTCCGTGGCTCTCCTAG 280 19 0 57.89 57.852 0.00 0.00 0.00 3.148 283 AGGTGCCCTGAAGAGCCT 239 18 0 61.11 61.150 0.00 0.00 30.39 3.150 284 AACATCTTCAGTGTGTACATGGC 120 23 0 43.48 60.176 0.00 0.00 0.00 3.176 285 GCATTCCCTTGGCCACATTCT 146 21 0 52.38 62.177 0.00 0.00 0.00 3.177 286 TGCCCTGAAGAGCCTCAT 236 18 0 55.56 58.776 0.00 0.00 0.00 3.224 287 TTTGTGGCTAGTCATGGAGGTG 256 22 0 50.00 61.229 0.00 0.00 0.00 3.229 288 GTTTGTGGCTAGTCATGGAGGT 257 22 0 50.00 61.229 0.00 0.00 0.00 3.229 289 AGGGAAGGTCTTCAGTGCATTC 162 22 0 50.00 61.232 12.62 0.00 41.71 3.232 290 GAAGGTCTTCAGTGCATTCCCT 159 22 0 50.00 61.232 6.42 0.00 39.78 3.232 291 GAGGTGCCCTGAAGAGCCT 240 19 0 63.16 62.234 0.00 0.00 32.62 3.234 292 AGGTGCCCTGAAGAGCCTC 239 19 0 63.16 62.234 0.00 0.00 30.39 3.234 293 CCTGAAGAGCCTCATTTTCCCA 233 22 0 50.00 61.237 0.00 0.00 0.00 3.237 294 TTCCCTTGGCCACATTCTTAGG 143 22 0 50.00 61.241 0.00 0.00 0.00 3.241 295 CCTTGGCCACATTCTTAGGGAA 140 22 0 50.00 61.241 10.05 0.00 38.69 3.241 296 TTCCCTTGGCCACATTCTT 143 19 0 47.37 57.758 0.00 0.00 0.00 3.242 297 ACACCCACAATGTCTTAGCTTTT 190 23 0 39.13 59.730 0.00 0.00 0.00 3.270 298 ACCCACAATGTCTTAGCTTTTGT 188 23 0 39.13 59.730 0.00 0.00 0.00 3.270 299 GTTACACCACCCCATTCTTTC 296 21 0 47.62 57.708 0.00 0.00 0.00 3.292 300 AGGGAACATCTTCAGTGTGTACA 124 23 0 43.48 60.303 0.00 0.00 0.00 3.303 301 TCATGGAGGTGCCCTGAA 245 18 0 55.56 58.689 0.00 0.00 39.11 3.311 302 AGTGTGTACATGGCGGCG 111 18 0 61.11 61.326 4.18 0.86 0.00 3.326 303 GCCACATTCTTAGGGAACATCTT 135 23 0 43.48 59.668 0.00 0.00 37.28 3.332 304 AGGGAACATCTTCAGTGTGTAC 124 22 0 45.45 58.658 0.00 0.00 0.00 3.342 305 CTAGCTGGGTTTGTGGCTA 265 19 0 52.63 57.656 0.00 0.00 36.85 3.344 306 TAGCTGGGTTTGTGGCTAG 264 19 0 52.63 57.656 0.00 0.00 36.85 3.344 307 TGGTACACCCACAATGTCTTAG 194 22 0 45.45 58.653 0.00 0.00 39.49 3.347 308 GTACACCCACAATGTCTTAGCTT 192 23 0 43.48 59.609 0.00 0.00 0.00 3.391 309 CCACAGCTGTAGGAAGTGT 214 19 0 52.63 57.592 23.93 0.00 0.00 3.408 310 AGCTGTAGGAAGTGTGTGG 210 19 0 52.63 57.592 0.00 0.00 0.00 3.408 311 TACACCCACAATGTCTTAGCT 191 21 0 42.86 57.587 0.00 0.00 0.00 3.413 312 CACCACCCCATTCTTTCCGTG 292 21 0 57.14 62.413 0.00 0.00 0.00 3.413 313 GCCACATTCTTAGGGAACATC 135 21 0 47.62 57.577 0.00 0.00 37.28 3.423 314 CAGTTACACCACCCCATTCTTTC 298 23 0 47.83 60.426 0.00 0.00 0.00 3.426 315 ATTAGGGAAGGTCTTCAGTGCAT 165 23 0 43.48 60.433 0.00 0.00 41.71 3.433 316 TGCATTCCCTTGGCCACATTC 147 21 0 52.38 62.445 0.00 0.00 0.00 3.445 317 TCATGGAGGTGCCCTGAAGAG 245 21 0 57.14 62.466 0.00 0.00 39.11 3.466 318 GTACACCCACAATGTCTTAGC 192 21 0 47.62 57.532 0.00 0.00 0.00 3.468 319 CTCATTTTCCCACAGCTGTAG 223 21 0 47.62 57.520 14.35 9.61 0.00 3.480 320 ATTCCCTTGGCCACATTCT 144 19 0 47.37 57.513 0.00 0.00 0.00 3.487 321 TGGTACACCCACAATGTCTTA 194 21 0 42.86 57.509 0.00 0.00 39.49 3.491 322 TTACACCACCCCATTCTTTCCG 295 22 0 50.00 61.493 0.00 0.00 0.00 3.493 323 AGGTCTTCAGTGCATTCCCTTG 157 22 0 50.00 61.495 0.00 0.00 0.00 3.495 324 TCAGTGCATTCCCTTGGC 151 18 0 55.56 58.490 0.00 0.00 0.00 3.510 325 GTATTAGGGAAGGTCTTCAGTGC 167 23 0 47.83 59.486 0.32 0.00 41.71 3.514 326 TGGAGGTGCCCTGAAGAGC 242 19 0 63.16 62.531 0.00 0.00 32.62 3.531 327 GGTGCCCTGAAGAGCCTCA 238 19 0 63.16 62.531 0.00 0.00 0.00 3.531 328 CATTCCCTTGGCCACATTC 145 19 0 52.63 57.456 0.00 0.00 0.00 3.544 329 CATTCCCTTGGCCACATTCTTAG 145 23 0 47.83 60.553 0.00 0.00 0.00 3.553 330 ATCTTCAGTGTGTACATGGCGG 117 22 0 50.00 61.605 0.00 0.00 0.00 3.605 331 CTAGCTGGGTTTGTGGCTAGTC 265 22 0 54.55 61.605 11.85 0.00 45.75 3.605 332 AGCTGGGTTTGTGGCTAGTCA 263 21 0 52.38 62.611 0.00 0.00 33.50 3.611 333 TCTTTCCGTGGCTCTCCT 281 18 0 55.56 58.384 0.00 0.00 0.00 3.616 334 TTAGGGAAGGTCTTCAGTGCATT 164 23 0 43.48 60.625 12.62 0.00 41.71 3.625 335 CAGTGTGTACATGGCGGCG 112 19 0 63.16 62.625 4.18 0.86 0.00 3.625 336 CACCCACAATGTCTTAGCTTTTG 189 23 0 43.48 59.370 0.00 0.00 0.00 3.630 337 GGGAACATCTTCAGTGTGTACAT 123 23 0 43.48 59.356 0.00 0.00 0.00 3.644 338 ATGGAGGTGCCCTGAAGA 243 18 0 55.56 58.353 0.00 0.00 32.62 3.647 339 CACCCACAATGTCTTAGCTTT 189 21 0 42.86 57.353 0.00 0.00 0.00 3.647 340 TTGTGGCTAGTCATGGAGG 255 19 0 52.63 57.343 0.00 0.00 0.00 3.657 341 TACACCCACAATGTCTTAGCTT 191 22 0 40.91 58.307 0.00 0.00 0.00 3.693 342 GTGCATTCCCTTGGCCACATT 148 21 0 52.38 62.720 0.00 0.00 0.00 3.720 343 TCTCCTAGCTGGGTTTGTG 269 19 0 52.63 57.267 11.17 0.68 36.53 3.733 344 TTCCCACAGCTGTAGGAAG 217 19 0 52.63 57.267 23.38 10.75 46.50 3.733 345 GTCATGGAGGTGCCCTGAAGA 246 21 0 57.14 62.737 0.00 0.00 42.92 3.737 346 CATGGAGGTGCCCTGAAG 244 18 0 61.11 58.257 0.00 0.00 35.27 3.743 347 AGTCATGGAGGTGCCCTGAAG 247 21 0 57.14 62.743 0.00 0.00 42.92 3.743 348 CTTCAGTGCATTCCCTTGGCC 153 21 0 57.14 62.759 0.00 0.00 0.00 3.759 349 GCAGTTACACCACCCCATTCTT 299 22 0 50.00 61.760 0.00 0.00 0.00 3.760 350 CTTCAGTGTGTACATGGCG 115 19 0 52.63 57.239 0.00 0.00 0.00 3.761 351 CTTCAGTGTGTACATGGCGGC 115 21 0 57.14 62.769 0.27 0.27 0.00 3.769 352 TAGGGAACATCTTCAGTGTGT 125 21 0 42.86 57.230 0.00 0.00 0.00 3.770 353 AGGGAACATCTTCAGTGTGTA 124 21 0 42.86 57.230 0.00 0.00 0.00 3.770 354 GGGAACATCTTCAGTGTGTAC 123 21 0 47.62 57.185 0.00 0.00 0.00 3.815 355 GCCTCATTTTCCCACAGCTGTA 225 22 0 50.00 61.817 20.84 2.94 0.00 3.817 356 TATTAGGGAAGGTCTTCAGTGC 166 22 0 45.45 58.182 0.32 0.00 41.71 3.818 357 TGCCCTGAAGAGCCTCATTTTC 236 22 0 50.00 61.821 0.00 0.00 0.00 3.821 358 CCCATTCTTTCCGTGGCT 286 18 0 55.56 58.169 0.00 0.00 0.00 3.831 359 TGGGTTTGTGGCTAGTCAT 260 19 0 47.37 57.152 0.00 0.00 0.00 3.848 360 AGAGCCTCATTTTCCCACA 228 19 0 47.37 57.144 0.00 0.00 0.00 3.856 361 GGGTTTGTGGCTAGTCATGGAG 259 22 0 54.55 61.874 0.00 0.00 0.00 3.874 362 GGTTTGTGGCTAGTCATGGAGG 258 22 0 54.55 61.874 0.00 0.00 0.00 3.874 363 CCCTGAAGAGCCTCATTTTCCC 234 22 0 54.55 61.888 0.00 0.00 0.00 3.888 364 ACACCACCCCATTCTTTCCGT 293 21 0 52.38 62.899 0.00 0.00 0.00 3.899 365 CCTCATTTTCCCACAGCTG 224 19 0 52.63 57.091 13.98 13.98 0.00 3.909 366 CTTCAGTGCATTCCCTTGG 153 19 0 52.63 57.091 0.00 0.00 0.00 3.909 367 GCTGGGTTTGTGGCTAGT 262 18 0 55.56 58.089 0.00 0.00 0.00 3.911 368 CACCCACAATGTCTTAGCTTTT 189 22 0 40.91 58.072 0.00 0.00 0.00 3.928 369 ACCCACAATGTCTTAGCTTTTG 188 22 0 40.91 58.072 0.00 0.00 0.00 3.928 370 CCCACAATGTCTTAGCTTTTGT 187 22 0 40.91 58.072 0.00 0.00 0.00 3.928 371 CTTAGGGAACATCTTCAGTGTGT 127 23 0 43.48 59.037 0.00 0.00 0.00 3.963 372 GGCTAGTCATGGAGGTGC 251 18 0 61.11 58.035 0.00 0.00 0.00 3.965 373 GCTAGTCATGGAGGTGCC 250 18 0 61.11 58.035 0.00 0.00 37.10 3.965 374 TAGGGAAGGTCTTCAGTGCATTC 163 23 0 47.83 60.999 12.62 0.00 41.71 3.999 375 TTAGGGAACATCTTCAGTGTGT 126 22 0 40.91 57.968 0.00 0.00 0.00 4.032 376 TACACCCACAATGTCTTAGCTTT 191 23 0 39.13 58.964 0.00 0.00 0.00 4.036 377 CCACAGCTGTAGGAAGTGTGTG 214 22 0 54.55 62.046 23.93 8.04 36.14 4.046 378 CACAGCTGTAGGAAGTGTGTGG 213 22 0 54.55 62.046 13.96 0.00 34.48 4.046 379 GTGTACATGGCGGCGGTC 108 18 0 66.67 62.075 4.86 0.00 0.00 4.075 380 GTGCCCTGAAGAGCCTCATTTT 237 22 0 50.00 62.084 0.00 0.00 0.00 4.084 381 AAGAGCCTCATTTTCCCACAGC 229 22 0 50.00 62.084 0.00 0.00 0.00 4.084 382 TCAGTGTGTACATGGCGG 113 18 0 55.56 57.888 0.00 0.00 0.00 4.112 383 TAGGGAACATCTTCAGTGTGTACA 125 24 0 41.67 60.116 0.00 0.00 0.00 4.116 384 CTGGGTTTGTGGCTAGTCATGG 261 22 0 54.55 62.134 0.00 0.00 0.00 4.134 385 GGGAAGGTCTTCAGTGCATTCC 161 22 0 54.55 62.135 12.62 8.87 41.71 4.135 386 GGAAGGTCTTCAGTGCATTCCC 160 22 0 54.55 62.135 12.62 0.00 41.71 4.135 387 GTACACCCACAATGTCTTAGCTTT 192 24 0 41.67 60.177 0.00 0.00 0.00 4.177 388 TTGGCCACATTCTTAGGGAACAT 138 23 0 43.48 61.205 0.00 0.00 37.28 4.205 389 TCTTAGGGAACATCTTCAGTGTG 128 23 0 43.48 58.784 0.00 0.00 0.00 4.216 390 CTAGCTGGGTTTGTGGCT 265 18 0 55.56 57.762 0.00 0.00 38.85 4.238 391 AGCTGGGTTTGTGGCTAG 263 18 0 55.56 57.762 0.00 0.00 33.50 4.238 392 TATTAGGGAAGGTCTTCAGTGCAT 166 24 0 41.67 60.239 1.14 0.00 41.71 4.239 393 TTCCCACAGCTGTAGGAAGTGT 217 22 0 50.00 62.244 23.38 0.00 46.50 4.244 394 GCAGTTACACCACCCCAT 299 18 0 55.56 57.752 0.00 0.00 0.00 4.248 395 ACCACCCCATTCTTTCCG 291 18 0 55.56 57.752 0.00 0.00 0.00 4.248 396 CCACCCCATTCTTTCCGT 290 18 0 55.56 57.752 0.00 0.00 0.00 4.248 397 ACCCCATTCTTTCCGTGG 288 18 0 55.56 57.752 0.00 0.00 0.00 4.248 398 GGTACACCCACAATGTCTTAGCT 193 23 0 47.83 61.250 0.00 0.00 0.00 4.250 399 CTCATTTTCCCACAGCTGTAGGA 223 23 0 47.83 61.252 29.48 28.23 39.52 4.252 400 TAGCTGGGTTTGTGGCTAGTCA 264 22 0 50.00 62.306 0.00 0.00 36.85 4.306 401 TAGTCATGGAGGTGCCCT 248 18 0 55.56 57.690 0.00 0.00 36.47 4.310 402 GGCCACATTCTTAGGGAACATCT 136 23 0 47.83 61.322 0.00 0.00 37.28 4.322 403 ATGGCGGCGGTCCCTTAA 102 18 0 61.11 62.333 6.72 0.00 0.00 4.333 404 TCTTAGGGAACATCTTCAGTGTGT 128 24 0 41.67 60.361 0.00 0.00 0.00 4.361 405 GTGTGTACATGGCGGCGG 110 18 0 66.67 62.378 10.10 0.00 0.00 4.378 406 GGTCTTCAGTGCATTCCCTTGG 156 22 0 54.55 62.394 0.00 0.00 0.00 4.394 407 CTAGTCATGGAGGTGCCCTGAA 249 22 0 54.55 62.434 0.00 0.00 42.92 4.434 408 TAGTCATGGAGGTGCCCTGAAG 248 22 0 54.55 62.434 0.00 0.00 42.92 4.434 409 TACACCCACAATGTCTTAGCTTTT 191 24 0 37.50 59.566 0.00 0.00 0.00 4.434 410 ACCCACAATGTCTTAGCTTTTGTA 188 24 0 37.50 59.566 0.00 0.00 0.00 4.434 411 AGTTACACCACCCCATTCTTTCC 297 23 0 47.83 61.452 0.00 0.00 0.00 4.452 412 GCCCTGAAGAGCCTCATTTTCC 235 22 0 54.55 62.461 0.00 0.00 0.00 4.461 413 TAGGGAACATCTTCAGTGTGTAC 125 23 0 43.48 58.533 0.00 0.00 0.00 4.467 414 GCCCTGAAGAGCCTCATT 235 18 0 55.56 57.510 0.00 0.00 0.00 4.490 415 ACAGCTGTAGGAAGTGTGTGGT 212 22 0 50.00 62.496 20.35 0.00 0.00 4.496 416 TGGTACACCCACAATGTCTTAGC 194 23 0 47.83 61.498 0.00 0.00 39.49 4.498 417 TTCTTTCCGTGGCTCTCC 282 18 0 55.56 57.466 0.00 0.00 0.00 4.534 418 GGGAACATCTTCAGTGTGTACATG 123 24 0 45.83 60.535 0.00 0.00 0.00 4.535 419 GGAACATCTTCAGTGTGTACATGG 122 24 0 45.83 60.535 0.00 0.00 0.00 4.535 420 CCACATTCTTAGGGAACATCTTCA 134 24 0 41.67 59.445 0.00 0.00 37.28 4.555 421 TGGCCACATTCTTAGGGAACATC 137 23 0 47.83 61.573 7.04 0.00 37.28 4.573 422 TCTTAGGGAACATCTTCAGTGT 128 22 0 40.91 57.422 0.00 0.00 0.00 4.578 423 TACACCACCCCATTCTTTCCGT 294 22 0 50.00 62.580 0.00 0.00 0.00 4.580 424 ATTCCCTTGGCCACATTCTTAGG 144 23 0 47.83 61.584 0.00 0.00 0.00 4.584 425 TGGGTTTGTGGCTAGTCATGGA 260 22 0 50.00 62.585 0.00 0.00 0.00 4.585 426 GCCACATTCTTAGGGAACATCTTC 135 24 0 45.83 60.598 0.00 0.00 37.28 4.598 427 TTCTTAGGGAACATCTTCAGTGTG 129 24 0 41.67 59.389 0.00 0.00 0.00 4.611 428 GGAACATCTTCAGTGTGTACATG 122 23 0 43.48 58.379 0.00 0.00 0.00 4.621 429 GAACATCTTCAGTGTGTACATGG 121 23 0 43.48 58.379 0.00 0.00 0.00 4.621 430 CTTAGGGAACATCTTCAGTGTG 127 22 0 45.45 57.369 0.00 0.00 0.00 4.631 431 GGGAAGGTCTTCAGTGCA 161 18 0 55.56 57.361 12.62 0.00 41.71 4.639 432 CATTCTTTCCGTGGCTCTCCTAG 284 23 0 52.17 61.668 0.00 0.00 0.00 4.668 433 AGGGAACATCTTCAGTGTGTACAT 124 24 0 41.67 60.668 0.00 0.00 0.00 4.668 434 GCATTCCCTTGGCCACATTCTT 146 22 0 50.00 62.682 0.00 0.00 0.00 4.682 435 CTCCTAGCTGGGTTTGTGGCTA 268 22 0 54.55 62.693 7.71 0.00 36.85 4.693 436 GCTGGGTTTGTGGCTAGTCATG 262 22 0 54.55 62.699 0.00 0.00 0.00 4.699 437 GAGCCTCATTTTCCCACAGCTG 227 22 0 54.55 62.705 13.98 13.98 0.00 4.705 438 CTAGTCATGGAGGTGCCC 249 18 0 61.11 57.294 0.00 0.00 35.38 4.706 439 AACATCTTCAGTGTGTACATGG 120 22 0 40.91 57.289 0.00 0.00 0.00 4.711 440 TGTATTAGGGAAGGTCTTCAGTG 168 23 0 43.48 58.264 0.32 0.00 41.71 4.736 441 GCCTCATTTTCCCACAGC 225 18 0 55.56 57.259 0.00 0.00 0.00 4.741 442 CTGAAGAGCCTCATTTTCCCACA 232 23 0 47.83 61.756 0.00 0.00 0.00 4.756 443 TGAAGAGCCTCATTTTCCCACAG 231 23 0 47.83 61.756 0.00 0.00 0.00 4.756 444 GAAGGTCTTCAGTGCATTCCCTT 159 23 0 47.83 61.757 6.42 2.57 39.78 4.757 445 CTTGGCCACATTCTTAGGGAACA 139 23 0 47.83 61.761 0.00 0.00 37.28 4.761 446 CAGCTGTAGGAAGTGTGTGGTAC 211 23 0 52.17 61.787 5.76 0.00 0.00 4.787 447 AGCTTTTGTATTAGGGAAGGTCT 174 23 0 39.13 58.173 0.00 0.00 0.00 4.827 448 TAGGGAACATCTTCAGTGTGTA 125 22 0 40.91 57.160 0.00 0.00 0.00 4.840 449 TGCAGTTACACCACCCCATTCT 300 22 0 50.00 62.852 0.00 0.00 0.00 4.852 450 TTAGGGAACATCTTCAGTGTGTAC 126 24 0 41.67 59.148 0.00 0.00 0.00 4.852 451 TCCCACAGCTGTAGGAAGTGTG 216 22 0 54.55 62.872 18.98 6.93 38.58 4.872 452 TTCTTAGGGAACATCTTCAGTGT 129 23 0 39.13 58.122 0.00 0.00 0.00 4.878 453 ACACCCACAATGTCTTAGCTTTTG 190 24 0 41.67 60.900 0.00 0.00 0.00 4.900 454 CACCCACAATGTCTTAGCTTTTGT 189 24 0 41.67 60.900 0.00 0.00 0.00 4.900 455 AGCTGGGTTTGTGGCTAGTCAT 263 22 0 50.00 62.911 0.00 0.00 33.50 4.911 456 AGAGCCTCATTTTCCCACAGCT 228 22 0 50.00 62.917 0.00 0.00 0.00 4.917 457 TGTGTACATGGCGGCGGT 109 18 0 61.11 62.923 11.28 11.28 0.00 4.923 458 GTCTTCAGTGCATTCCCTTGGC 155 22 0 54.55 62.957 0.00 0.00 0.00 4.957 459 AGGGAAGGTCTTCAGTGC 162 18 0 55.56 57.027 12.62 0.00 41.71 4.973 460 GGAACATCTTCAGTGTGTACAT 122 22 0 40.91 57.024 0.00 0.00 0.00 4.976 461 ATTAGGGAAGGTCTTCAGTGCATT 165 24 0 41.67 60.979 0.00 0.00 41.71 4.979 462 TCCTAGCTGGGTTTGTGG 267 18 0 55.56 57.016 9.65 0.00 36.53 4.984 463 TCCCTTGGCCACATTCTTAGGG 142 22 0 54.55 62.988 25.97 25.97 0.00 4.988 464 CCCTTGGCCACATTCTTAGGGA 141 22 0 54.55 62.988 27.33 0.00 32.24 4.988 465 GCTTTTGTATTAGGGAAGGTCTTCA 173 25 0 40.00 60.003 12.62 0.00 41.71 5.003 466 AAGGTCTTCAGTGCATTCCCTTG 158 23 0 47.83 62.008 2.24 0.00 0.00 5.008 467 CCCACAATGTCTTAGCTTTTGTA 187 23 0 39.13 57.972 0.00 0.00 0.00 5.028 468 TGTATTAGGGAAGGTCTTCAGTGC 168 24 0 45.83 61.028 0.00 0.00 41.71 5.028 469 GTATTAGGGAAGGTCTTCAGTGCA 167 24 0 45.83 61.028 0.00 0.00 41.71 5.028 470 ACCCACAATGTCTTAGCTTTTGTAT 188 25 0 36.00 59.943 0.00 0.00 0.00 5.057 471 CTTAGGGAACATCTTCAGTGTGTAC 127 25 0 44.00 60.064 0.00 0.00 0.00 5.064 472 GAACATCTTCAGTGTGTACATGGC 121 24 0 45.83 61.065 0.00 0.00 0.00 5.065 473 ACATCTTCAGTGTGTACATGGCG 119 23 0 47.83 62.095 0.00 0.00 0.00 5.095 474 CTTAGGGAACATCTTCAGTGTGTA 127 24 0 41.67 58.902 0.00 0.00 0.00 5.098 475 TTGTATTAGGGAAGGTCTTCAGTG 169 24 0 41.67 58.895 0.32 0.00 41.71 5.105 476 CCTCATTTTCCCACAGCTGTAGG 224 23 0 52.17 62.121 23.28 23.28 0.00 5.121 477 TTAGGGAACATCTTCAGTGTGTA 126 23 0 39.13 57.870 0.00 0.00 0.00 5.130 478 CCACATTCTTAGGGAACATCTTC 134 23 0 43.48 57.850 0.00 0.00 37.28 5.150 479 TCTTAGGGAACATCTTCAGTGTGTA 128 25 0 40.00 60.179 0.00 0.00 0.00 5.179 480 AGCTTTTGTATTAGGGAAGGTCTT 174 24 0 37.50 58.816 0.00 0.00 0.00 5.184 481 ACAGCTGTAGGAAGTGTGTGGTA 212 23 0 47.83 62.210 20.35 0.00 0.00 5.210 482 AGCTGTAGGAAGTGTGTGGTACA 210 23 0 47.83 62.210 0.00 0.00 37.17 5.210 483 CACATTCTTAGGGAACATCTTCAGT 133 25 0 40.00 59.770 0.00 0.00 37.28 5.230 484 ACATTCTTAGGGAACATCTTCAGTG 132 25 0 40.00 59.770 0.00 0.00 37.28 5.230 485 CATTCTTAGGGAACATCTTCAGTGT 131 25 0 40.00 59.770 0.00 0.00 37.28 5.230 486 ATTCTTAGGGAACATCTTCAGTGTG 130 25 0 40.00 59.770 0.00 0.00 37.28 5.230 487 AGCTTTTGTATTAGGGAAGGTCTTC 174 25 0 40.00 59.768 2.83 2.83 39.06 5.232 488 GCAGTTACACCACCCCATTCTTT 299 23 0 47.83 62.263 0.00 0.00 0.00 5.263 489 CCACATTCTTAGGGAACATCTTCAG 134 25 0 44.00 60.353 0.00 0.00 37.28 5.353 490 GTTTGTGGCTAGTCATGGAGGTG 257 23 0 52.17 62.354 0.00 0.00 0.00 5.354 491 CCTGAAGAGCCTCATTTTCCCAC 233 23 0 52.17 62.371 0.00 0.00 0.00 5.371 492 CCTTGGCCACATTCTTAGGGAAC 140 23 0 52.17 62.379 10.05 0.00 37.28 5.379 493 GCATTCCCTTGGCCACATTCTTA 146 23 0 47.83 62.390 0.00 0.00 0.00 5.390 494 TTGTATTAGGGAAGGTCTTCAGT 169 23 0 39.13 57.591 12.62 2.63 41.71 5.409 495 ACATTCTTAGGGAACATCTTCAGT 132 24 0 37.50 58.574 0.00 0.00 37.28 5.426 496 ATTCTTAGGGAACATCTTCAGTGT 130 24 0 37.50 58.574 0.00 0.00 37.28 5.426 497 TAGGGAACATCTTCAGTGTGTACAT 125 25 0 40.00 60.474 0.00 0.00 0.00 5.474 498 GCTTTTGTATTAGGGAAGGTCTTC 173 24 0 41.67 58.492 2.83 2.83 39.06 5.508 499 TTAGGGAAGGTCTTCAGTGCATTC 164 24 0 45.83 61.513 12.62 0.00 41.71 5.513 500 TTTGTATTAGGGAAGGTCTTCAGTG 170 25 0 40.00 59.475 0.32 0.00 41.71 5.525 501 GCTTTTGTATTAGGGAAGGTCTT 173 23 0 39.13 57.441 0.00 0.00 0.00 5.559 502 CCCACAATGTCTTAGCTTTTGTAT 187 24 0 37.50 58.421 0.00 0.00 0.00 5.579 503 GTTACACCACCCCATTCTTTCCG 296 23 0 52.17 62.605 0.00 0.00 0.00 5.605 504 TAGCTGGGTTTGTGGCTAGTCAT 264 23 0 47.83 62.606 0.00 0.00 36.85 5.606 505 TTAGGGAACATCTTCAGTGTGTACA 126 25 0 40.00 60.649 0.00 0.00 0.00 5.649 506 GCCTCATTTTCCCACAGCTGTAG 225 23 0 52.17 62.665 14.35 9.61 0.00 5.665 507 CATCTTCAGTGTGTACATGGCGG 118 23 0 52.17 62.694 0.00 0.00 0.00 5.694 508 TACACCCACAATGTCTTAGCTTTTG 191 25 0 40.00 60.700 0.00 0.00 0.00 5.700 509 CACCCACAATGTCTTAGCTTTTGTA 189 25 0 40.00 60.700 0.00 0.00 0.00 5.700 510 GTACACCCACAATGTCTTAGCTTTT 192 25 0 40.00 60.700 0.00 0.00 0.00 5.700 511 TTTGTATTAGGGAAGGTCTTCAGT 170 24 0 37.50 58.259 12.62 2.63 41.71 5.741 512 CACATTCTTAGGGAACATCTTCAG 133 24 0 41.67 58.254 0.00 0.00 37.28 5.746 513 CATTCTTAGGGAACATCTTCAGTG 131 24 0 41.67 58.254 0.00 0.00 37.28 5.746 514 GGTACACCCACAATGTCTTAGCTT 193 24 0 45.83 61.752 0.00 0.00 0.00 5.752 515 TATTAGGGAAGGTCTTCAGTGCATT 166 25 0 40.00 60.772 0.99 0.00 41.71 5.772 516 CACATTCTTAGGGAACATCTTCA 133 23 0 39.13 57.198 0.00 0.00 37.28 5.802 517 GGCCACATTCTTAGGGAACATCTT 136 24 0 45.83 61.826 0.00 0.00 37.28 5.826 518 TTCTTAGGGAACATCTTCAGTGTGT 129 25 0 40.00 60.886 0.00 0.00 0.00 5.886 519 GTGCCCTGAAGAGCCTCATTTTC 237 23 0 52.17 62.913 0.00 0.00 0.00 5.913 520 GAAGAGCCTCATTTTCCCACAGC 230 23 0 52.17 62.913 0.00 0.00 0.00 5.913 521 TAGCTTTTGTATTAGGGAAGGTCT 175 24 0 37.50 58.071 0.00 0.00 0.00 5.929 522 CCATTCTTTCCGTGGCTCTCCTA 285 23 0 52.17 62.953 0.00 0.00 0.00 5.953 523 CCCACAATGTCTTAGCTTTTGTATT 187 25 0 36.00 59.016 0.00 0.00 0.00 5.984 524 TTTTGTATTAGGGAAGGTCTTCAGTG 171 26 0 38.46 60.011 0.32 0.00 41.71 6.011 525 TTGGCCACATTCTTAGGGAACATC 138 24 0 45.83 62.064 0.00 0.00 37.28 6.064 526 CTTGGCCACATTCTTAGGGAACAT 139 24 0 45.83 62.068 0.00 0.00 37.28 6.068 527 TTTTGTATTAGGGAAGGTCTTCAGT 171 25 0 36.00 58.872 12.62 2.63 41.71 6.128 528 TGTCTTAGCTTTTGTATTAGGGAAGG 180 26 0 38.46 59.842 0.00 0.00 0.00 6.158 529 GTCTTAGCTTTTGTATTAGGGAAGGT 179 26 0 38.46 59.841 0.00 0.00 0.00 6.159 530 CTTTTGTATTAGGGAAGGTCTTCAGT 172 26 0 38.46 59.785 12.62 2.63 41.71 6.215 531 TCTTAGCTTTTGTATTAGGGAAGGT 178 25 0 36.00 58.693 0.00 0.00 0.00 6.307 532 TTAGCTTTTGTATTAGGGAAGGTCT 176 25 0 36.00 58.693 0.00 0.00 0.00 6.307 533 TAGCTTTTGTATTAGGGAAGGTCTT 175 25 0 36.00 58.693 0.00 0.00 0.00 6.307 534 GTATTAGGGAAGGTCTTCAGTGCAT 167 25 0 44.00 61.350 1.14 0.00 41.71 6.350 535 TCTTAGCTTTTGTATTAGGGAAGGTC 178 26 0 38.46 59.616 0.00 0.00 0.00 6.384 536 TAGCTTTTGTATTAGGGAAGGTCTTC 175 26 0 38.46 59.616 2.83 2.83 39.06 6.384 537 CTTAGCTTTTGTATTAGGGAAGGTCT 177 26 0 38.46 59.614 0.00 0.00 0.00 6.386 538 ACCCACAATGTCTTAGCTTTTGTATT 188 26 0 34.62 60.463 0.00 0.00 0.00 6.463 539 TTGTATTAGGGAAGGTCTTCAGTGC 169 25 0 44.00 61.521 0.00 0.00 41.71 6.521 540 CAGTTACACCACCCCATTCTTTCC 298 24 0 50.00 62.531 0.00 0.00 0.00 6.531 541 AACATCTTCAGTGTGTACATGGCG 120 24 0 45.83 62.553 0.00 0.00 0.00 6.553 542 CTGAAGAGCCTCATTTTCCCACAG 232 24 0 50.00 62.576 0.00 0.00 0.00 6.576 543 GTCTTAGCTTTTGTATTAGGGAAGG 179 25 0 40.00 58.380 0.00 0.00 0.00 6.620 544 CTTAGCTTTTGTATTAGGGAAGGTC 177 25 0 40.00 58.380 0.00 0.00 0.00 6.620 545 TTAGCTTTTGTATTAGGGAAGGTC 176 24 0 37.50 57.369 0.00 0.00 0.00 6.631 546 CTTAGCTTTTGTATTAGGGAAGGT 177 24 0 37.50 57.363 0.00 0.00 0.00 6.637 547 CATTCCCTTGGCCACATTCTTAGG 145 24 0 50.00 62.658 0.00 0.00 0.00 6.658 548 CTTTTGTATTAGGGAAGGTCTTCAG 172 25 0 40.00 58.320 12.62 0.00 41.71 6.680 549 TTCTTAGGGAACATCTTCAGTGTGTA 129 26 0 38.46 60.690 0.00 0.00 0.00 6.690 550 CTTTTGTATTAGGGAAGGTCTTCA 172 24 0 37.50 57.304 12.62 0.00 41.71 6.696 551 TTTTGTATTAGGGAAGGTCTTCAG 171 24 0 37.50 57.304 12.62 0.00 41.71 6.696 552 TTAGCTTTTGTATTAGGGAAGGTCTT 176 26 0 34.62 59.266 0.00 0.00 0.00 6.734 553 AGGGAACATCTTCAGTGTGTACATG 124 25 0 44.00 61.749 0.00 0.00 0.00 6.749 554 TGGTACACCCACAATGTCTTAGCT 194 24 0 45.83 62.751 0.00 0.00 39.49 6.751 555 GAAGGTCTTCAGTGCATTCCCTTG 159 24 0 50.00 62.809 6.43 0.00 39.78 6.809 556 ATTAGGGAAGGTCTTCAGTGCATTC 165 25 0 44.00 61.816 0.00 0.00 41.71 6.816 557 TGGCCACATTCTTAGGGAACATCT 137 24 0 45.83 62.833 7.04 0.00 37.28 6.833 558 CACATTCTTAGGGAACATCTTCAGTG 133 26 0 42.31 60.846 0.00 0.00 37.28 6.846 559 CATTCTTAGGGAACATCTTCAGTGTG 131 26 0 42.31 60.846 0.00 0.00 37.28 6.846 560 GCTTTTGTATTAGGGAAGGTCTTCAG 173 26 0 42.31 60.848 12.62 0.00 41.71 6.848 561 TCTTAGCTTTTGTATTAGGGAAGG 178 24 0 37.50 57.117 0.00 0.00 0.00 6.883 562 TTAGGGAACATCTTCAGTGTGTACAT 126 26 0 38.46 60.974 0.00 0.00 0.00 6.974 563 CACCCACAATGTCTTAGCTTTTGTAT 189 26 0 38.46 61.018 0.00 0.00 0.00 7.018 564 GCCACATTCTTAGGGAACATCTTCA 135 25 0 44.00 62.045 0.00 0.00 37.28 7.045 565 CCCACAATGTCTTAGCTTTTGTATTA 187 26 0 34.62 58.892 0.00 0.00 0.00 7.108 566 CTTAGCTTTTGTATTAGGGAAGGTCTT 177 27 0 37.04 60.127 0.00 0.00 0.00 7.127 567 TTAGCTTTTGTATTAGGGAAGGTCTTC 176 27 0 37.04 60.127 2.83 2.83 39.06 7.127 568 ACAATGTCTTAGCTTTTGTATTAGGGA 184 27 0 33.33 59.853 0.00 0.00 0.00 7.147 569 ATGTCTTAGCTTTTGTATTAGGGAAGG 181 27 0 37.04 60.181 0.00 0.00 0.00 7.181 570 ACATTCTTAGGGAACATCTTCAGTGT 132 26 0 38.46 61.203 0.00 0.00 37.28 7.203 571 ATTCTTAGGGAACATCTTCAGTGTGT 130 26 0 38.46 61.203 0.00 0.00 37.28 7.203 572 AGCTTTTGTATTAGGGAAGGTCTTCA 174 26 0 38.46 61.206 12.62 0.00 41.71 7.206 573 GGTACACCCACAATGTCTTAGCTTT 193 25 0 44.00 62.214 0.00 0.00 0.00 7.214 574 TGTCTTAGCTTTTGTATTAGGGAAG 180 25 0 36.00 57.783 0.00 0.00 0.00 7.217 575 TCTTAGGGAACATCTTCAGTGTGTAC 128 26 0 42.31 61.245 0.00 0.00 0.00 7.245 576 CCCACAATGTCTTAGCTTTTGTATTAG 187 27 0 37.04 59.754 0.00 0.00 0.00 7.246 577 CCACAATGTCTTAGCTTTTGTATTAGG 186 27 0 37.04 59.754 0.00 0.00 0.00 7.246 578 CACAATGTCTTAGCTTTTGTATTAGGG 185 27 0 37.04 59.754 0.00 0.00 0.00 7.246 579 ACCCACAATGTCTTAGCTTTTGTATTA 188 27 0 33.33 60.290 0.00 0.00 0.00 7.290 580 ACAATGTCTTAGCTTTTGTATTAGGG 184 26 0 34.62 58.663 0.00 0.00 0.00 7.337 581 ACACCCACAATGTCTTAGCTTTTGT 190 25 0 40.00 62.343 0.00 0.00 0.00 7.343 582 CTTAGGGAACATCTTCAGTGTGTACA 127 26 0 42.31 61.468 0.00 0.00 0.00 7.468 583 TGTATTAGGGAAGGTCTTCAGTGCA 168 25 0 44.00 62.481 0.60 0.00 41.71 7.481 584 TAGGGAACATCTTCAGTGTGTACATG 125 26 0 42.31 61.524 0.00 0.00 0.00 7.524 585 GGGAACATCTTCAGTGTGTACATGG 123 25 0 48.00 62.539 0.00 0.00 0.00 7.539 586 CAATGTCTTAGCTTTTGTATTAGGGA 183 26 0 34.62 58.438 0.00 0.00 0.00 7.562 587 TATTAGGGAAGGTCTTCAGTGCATTC 166 26 0 42.31 61.588 0.99 0.00 41.71 7.588 588 GGCCACATTCTTAGGGAACATCTTC 136 25 0 48.00 62.611 0.00 0.00 37.28 7.611 589 GTCTTAGCTTTTGTATTAGGGAAGGTC 179 27 0 40.74 60.665 0.00 0.00 0.00 7.665 590 GTACACCCACAATGTCTTAGCTTTTG 192 26 0 42.31 61.721 0.00 0.00 0.00 7.721 591 CCACATTCTTAGGGAACATCTTCAGT 134 26 0 42.31 61.759 0.00 0.00 37.28 7.759 592 AATGTCTTAGCTTTTGTATTAGGGA 182 25 0 32.00 57.218 0.00 0.00 0.00 7.782 593 ATGTCTTAGCTTTTGTATTAGGGAA 181 25 0 32.00 57.218 0.00 0.00 0.00 7.782 594 TCTTAGCTTTTGTATTAGGGAAGGTCT 178 27 0 37.04 60.787 0.00 0.00 0.00 7.787 595 ATGTCTTAGCTTTTGTATTAGGGAAG 181 26 0 34.62 58.208 0.00 0.00 0.00 7.792 596 GTATTAGGGAAGGTCTTCAGTGCATT 167 26 0 42.31 61.813 0.00 0.00 41.71 7.813 597 CAATGTCTTAGCTTTTGTATTAGGG 183 25 0 36.00 57.176 0.00 0.00 0.00 7.824 598 CTTTTGTATTAGGGAAGGTCTTCAGTG 172 27 0 40.74 60.826 0.32 0.00 41.71 7.826 599 CTTGGCCACATTCTTAGGGAACATC 139 25 0 48.00 62.840 0.00 0.00 37.28 7.840 600 TTTGTATTAGGGAAGGTCTTCAGTGC 170 26 0 42.31 61.976 0.00 0.00 41.71 7.976 601 ATTCTTAGGGAACATCTTCAGTGTGTA 130 27 0 37.04 61.003 0.00 0.00 37.28 8.003 602 TAGCTTTTGTATTAGGGAAGGTCTTCA 175 27 0 37.04 61.005 12.62 0.00 41.71 8.005 603 CAATGTCTTAGCTTTTGTATTAGGGAA 183 27 0 33.33 58.991 0.00 0.00 0.00 8.009 604 TACACCCACAATGTCTTAGCTTTTGT 191 26 0 38.46 62.095 0.00 0.00 0.00 8.095 605 ACACCCACAATGTCTTAGCTTTTGTA 190 26 0 38.46 62.095 0.00 0.00 0.00 8.095 606 AATGTCTTAGCTTTTGTATTAGGGAA 182 26 0 30.77 57.847 0.00 0.00 0.00 8.153 607 CCACAATGTCTTAGCTTTTGTATTAG 186 26 0 34.62 57.804 0.00 0.00 0.00 8.196 608 CACAATGTCTTAGCTTTTGTATTAGG 185 26 0 34.62 57.804 0.00 0.00 0.00 8.196 609 TGTCTTAGCTTTTGTATTAGGGAAGGT 180 27 0 37.04 61.225 0.00 0.00 0.00 8.225 610 AATGTCTTAGCTTTTGTATTAGGGAAG 182 27 0 33.33 58.771 0.00 0.00 0.00 8.229 611 CACCCACAATGTCTTAGCTTTTGTATT 189 27 0 37.04 61.473 0.00 0.00 0.00 8.473 612 GGTACACCCACAATGTCTTAGCTTTT 193 26 0 42.31 62.641 0.00 0.00 0.00 8.641 613 TTCTTAGGGAACATCTTCAGTGTGTAC 129 27 0 40.74 61.693 0.00 0.00 0.00 8.693 614 TGTATTAGGGAAGGTCTTCAGTGCAT 168 26 0 42.31 62.741 0.60 0.00 41.71 8.741 615 CTTAGGGAACATCTTCAGTGTGTACAT 127 27 0 40.74 61.750 0.00 0.00 0.00 8.750 616 GCCACATTCTTAGGGAACATCTTCAG 135 26 0 46.15 62.791 0.00 0.00 37.28 8.791 617 TACACCCACAATGTCTTAGCTTTTGTA 191 27 0 37.04 61.865 0.00 0.00 0.00 8.865 618 TTGTATTAGGGAAGGTCTTCAGTGCA 169 26 0 42.31 62.904 0.60 0.00 41.71 8.904 619 TTAGGGAACATCTTCAGTGTGTACATG 126 27 0 40.74 61.961 0.00 0.00 0.00 8.961 620 AGCTTTTGTATTAGGGAAGGTCTTCAG 174 27 0 40.74 61.974 12.62 0.00 41.71 8.974 621 CACATTCTTAGGGAACATCTTCAGTGT 133 27 0 40.74 62.180 0.00 0.00 37.28 9.180 622 ACATTCTTAGGGAACATCTTCAGTGTG 132 27 0 40.74 62.180 0.00 0.00 37.28 9.180 623 CATTCTTAGGGAACATCTTCAGTGTGT 131 27 0 40.74 62.180 0.00 0.00 37.28 9.180 624 GCTTTTGTATTAGGGAAGGTCTTCAGT 173 27 0 40.74 62.187 2.51 0.00 41.71 9.187 625 ACACCCACAATGTCTTAGCTTTTGTAT 190 27 0 37.04 62.357 0.00 0.00 0.00 9.357 626 TTTTGTATTAGGGAAGGTCTTCAGTGC 171 27 0 40.74 62.398 0.00 0.00 41.71 9.398 627 GTATTAGGGAAGGTCTTCAGTGCATTC 167 27 0 44.44 62.540 0.00 0.00 41.71 9.540 628 TCTTAGGGAACATCTTCAGTGTGTACA 128 27 0 40.74 62.581 0.00 0.00 0.00 9.581 629 CCACATTCTTAGGGAACATCTTCAGTG 134 27 0 44.44 62.703 0.00 0.00 37.28 9.703 primer3-2.3.6/test/primer1_th_list_last/SP01-21.txt.for0000664000077300007730000010144012231156243021414 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TGTCTTGTGGGTGTGTCTGT 61 20 0 50.00 60.024 0.00 0.00 0.00 0.024 1 TGTGTCTTGTGGGTGTGTCT 59 20 0 50.00 60.024 0.00 0.00 0.00 0.024 2 GTGTCTTGTGGGTGTGTCTG 60 20 0 55.00 59.605 0.00 0.00 0.00 0.395 3 GTCTTGTGGGTGTGTCTGTT 62 20 0 50.00 58.879 0.00 0.00 0.00 1.121 4 TGTCTTGTGGGTGTGTCTGTT 61 21 0 47.62 60.657 0.00 0.00 0.00 1.657 5 GAACATGTGTGGCATTGTGT 1 20 0 45.00 58.108 0.00 0.00 35.88 1.892 6 AGCTTCTTGACTGTGTGTTGT 129 21 0 42.86 58.935 0.00 0.00 0.00 2.065 7 AGCTTCTTGACTGTGTGTTGTG 129 22 0 45.45 60.239 0.00 0.00 0.00 2.239 8 GTCTTGTGGGTGTGTCTGTTA 62 21 0 47.62 58.734 0.00 0.00 0.00 2.266 9 TGTGTCTTGTGGGTGTGTCTG 59 21 0 52.38 61.332 0.00 0.00 0.00 2.332 10 GTGTCTTGTGGGTGTGTCTGT 60 21 0 52.38 61.333 0.00 0.00 0.00 2.333 11 TGTGGGTGTGTCTGTTAAACA 66 21 0 42.86 58.579 0.00 0.00 0.00 2.421 12 TGTCTTGTGGGTGTGTCTGTTA 61 22 0 45.45 60.439 0.00 0.00 0.00 2.439 13 TGTGTCTTGTGGGTGTGTC 59 19 0 52.63 58.440 0.00 0.00 0.00 2.560 14 GTCTTGTGGGTGTGTCTGTTAA 62 22 0 45.45 59.389 0.00 0.00 0.00 2.611 15 TCTTGTGGGTGTGTCTGTTA 63 20 0 45.00 57.250 0.00 0.00 0.00 2.750 16 TTGTGGGTGTGTCTGTTAAACA 65 22 0 40.91 59.248 0.00 0.00 0.00 2.752 17 TGTCTTGTGGGTGTGTCTG 61 19 0 52.63 58.133 0.00 0.00 0.00 2.867 18 GTCTTGTGGGTGTGTCTGT 62 19 0 52.63 58.131 0.00 0.00 0.00 2.869 19 GTGTCTTGTGGGTGTGTCT 60 19 0 52.63 58.131 0.00 0.00 0.00 2.869 20 AGCTTCTTGACTGTGTGTTG 129 20 0 45.00 57.112 0.00 0.00 0.00 2.888 21 TGTAGCTTCTTGACTGTGTGT 126 21 0 42.86 58.106 0.00 0.00 0.00 2.894 22 TGGGTGTGTCTGTTAAACATGT 68 22 0 40.91 59.046 0.00 0.00 0.00 2.954 23 TGTGGGTGTGTCTGTTAAACAT 66 22 0 40.91 59.046 0.00 0.00 0.00 2.954 24 TCTTGTGGGTGTGTCTGTTAA 63 21 0 42.86 58.017 0.00 0.00 0.00 2.983 25 GAACATGTGTGGCATTGTGTA 1 21 0 42.86 57.999 0.00 0.00 35.88 3.001 26 TGGATGTAGCTTCTTGACTGTG 122 22 0 45.45 58.995 0.00 0.00 0.00 3.005 27 GGATGTAGCTTCTTGACTGTGT 123 22 0 45.45 58.995 0.00 0.00 0.00 3.005 28 TCTTGTGGGTGTGTCTGTTAAAC 63 23 0 43.48 59.988 0.00 0.00 0.00 3.012 29 GTCTTGTGGGTGTGTCTGTTAAA 62 23 0 43.48 59.988 0.00 0.00 0.00 3.012 30 GTAGCTTCTTGACTGTGTGTTGT 127 23 0 43.48 60.052 0.00 0.00 0.00 3.052 31 TAGCTTCTTGACTGTGTGTTGTG 128 23 0 43.48 60.052 0.00 0.00 0.00 3.052 32 TGTAGCTTCTTGACTGTGTGTTG 126 23 0 43.48 60.052 0.00 0.00 0.00 3.052 33 TGTGTGTTGTGTATGGATGTAGC 109 23 0 43.48 59.926 0.00 0.00 0.00 3.074 34 TAGCTTCTTGACTGTGTGTTGT 128 22 0 40.91 58.793 0.00 0.00 0.00 3.207 35 TGTAGCTTCTTGACTGTGTGTT 126 22 0 40.91 58.793 0.00 0.00 0.00 3.207 36 GGATGTAGCTTCTTGACTGTGTG 123 23 0 47.83 60.238 0.00 0.00 0.00 3.238 37 CTTGTGGGTGTGTCTGTTAAACA 64 23 0 43.48 60.238 0.00 0.00 0.00 3.238 38 TCTTGTGGGTGTGTCTGTTAAA 63 22 0 40.91 58.714 0.00 0.00 0.00 3.286 39 TGGGTGTGTCTGTTAAACATGTG 68 23 0 43.48 60.301 0.00 0.00 0.00 3.301 40 TGTGGGTGTGTCTGTTAAACATG 66 23 0 43.48 60.301 0.00 0.00 0.00 3.301 41 GGGTGTGTCTGTTAAACATGTGT 69 23 0 43.48 60.301 0.00 0.00 0.00 3.301 42 GTGGGTGTGTCTGTTAAACATGT 67 23 0 43.48 60.301 0.00 0.00 0.00 3.301 43 GGGTGTGTCTGTTAAACATGTG 69 22 0 45.45 58.689 0.00 0.00 0.00 3.311 44 GTGGGTGTGTCTGTTAAACATG 67 22 0 45.45 58.689 0.00 0.00 0.00 3.311 45 GTGTGTTGTGTATGGATGTAGCT 110 23 0 43.48 59.675 0.00 0.00 0.00 3.325 46 TGTGTCTGTTAAACATGTGTGGT 72 23 0 39.13 59.671 0.00 0.00 0.00 3.329 47 TTGTGGGTGTGTCTGTTAAACAT 65 23 0 39.13 59.668 0.00 0.00 0.00 3.332 48 CTTGTGGGTGTGTCTGTTAAAC 64 22 0 45.45 58.625 0.00 0.00 0.00 3.375 49 GATGTAGCTTCTTGACTGTGTGT 124 23 0 43.48 59.615 0.00 0.00 0.00 3.385 50 ACATGTGTGGCATTGTGTATACA 3 23 0 39.13 59.607 7.27 4.43 35.88 3.393 51 TGGATGTAGCTTCTTGACTGT 122 21 0 42.86 57.597 0.00 0.00 0.00 3.403 52 ATGTAGCTTCTTGACTGTGTGT 125 22 0 40.91 58.590 0.00 0.00 0.00 3.410 53 AAACATGTGTGGTATTGTGCA 82 21 0 38.10 57.554 0.00 0.00 0.00 3.446 54 ACATGTGTGTTGTGTATGGATGT 106 23 0 39.13 59.542 0.00 0.00 34.85 3.458 55 TTGTGGGTGTGTCTGTTAAAC 65 21 0 42.86 57.507 0.00 0.00 0.00 3.493 56 GAACATGTGTGGCATTGTGTAT 1 22 0 40.91 58.480 0.00 0.00 35.88 3.520 57 TGTGTGGCATTGTGTATACAC 6 21 0 42.86 57.451 26.66 26.66 46.84 3.549 58 GTAGCTTCTTGACTGTGTGTTG 127 22 0 45.45 58.445 0.00 0.00 0.00 3.555 59 ATGGATGTAGCTTCTTGACTGTG 121 23 0 43.48 59.421 0.00 0.00 0.00 3.579 60 TGTGTTGTGTATGGATGTAGCT 111 22 0 40.91 58.384 0.00 0.00 0.00 3.616 61 TGGATGTAGCTTCTTGACTGTGT 122 23 0 43.48 60.620 0.00 0.00 0.00 3.620 62 AACATGTGTGGTATTGTGCAT 83 21 0 38.10 57.337 0.00 0.00 0.00 3.663 63 AACATGTGTGGCATTGTGTAT 2 21 0 38.10 57.337 0.00 0.00 35.88 3.663 64 TCTTGTGGGTGTGTCTGTT 63 19 0 47.37 57.331 0.00 0.00 0.00 3.669 65 GTGTGTCTGTTAAACATGTGTGG 71 23 0 43.48 59.315 0.00 0.00 0.00 3.685 66 GGTGTGTCTGTTAAACATGTGTG 70 23 0 43.48 59.315 0.00 0.00 0.00 3.685 67 GTGTGTTGTGTATGGATGTAGC 110 22 0 45.45 58.308 0.00 0.00 0.00 3.692 68 TGGGTGTGTCTGTTAAACATG 68 21 0 42.86 57.293 0.00 0.00 0.00 3.707 69 GGGTGTGTCTGTTAAACATGT 69 21 0 42.86 57.292 0.00 0.00 0.00 3.708 70 GTGGGTGTGTCTGTTAAACAT 67 21 0 42.86 57.292 0.00 0.00 0.00 3.708 71 GGATGTAGCTTCTTGACTGTG 123 21 0 47.62 57.263 0.00 0.00 0.00 3.737 72 CATGTGTGGCATTGTGTATACAC 4 23 0 43.48 59.252 26.66 26.66 41.05 3.748 73 ATGTAGCTTCTTGACTGTGTGTT 125 23 0 39.13 59.228 0.00 0.00 0.00 3.772 74 CTTGTGGGTGTGTCTGTTAAA 64 21 0 42.86 57.225 0.00 0.00 0.00 3.775 75 GTGTCTTGTGGGTGTGTCTGTT 60 22 0 50.00 61.870 0.00 0.00 0.00 3.870 76 ATGGATGTAGCTTCTTGACTGT 121 22 0 40.91 58.107 0.00 0.00 0.00 3.893 77 AAACATGTGTGGTATTGTGCAT 82 22 0 36.36 58.061 0.00 0.00 0.00 3.939 78 TAGCTTCTTGACTGTGTGTTG 128 21 0 42.86 57.047 0.00 0.00 0.00 3.953 79 GTAGCTTCTTGACTGTGTGTT 127 21 0 42.86 57.045 0.00 0.00 0.00 3.955 80 TGTGTTGTGTATGGATGTAGCTT 111 23 0 39.13 59.034 0.00 0.00 0.00 3.966 81 TGTGTCTGTTAAACATGTGTGG 72 22 0 40.91 58.026 0.00 0.00 0.00 3.974 82 GTGTCTGTTAAACATGTGTGGT 73 22 0 40.91 58.025 0.00 0.00 0.00 3.975 83 GGTGTGTCTGTTAAACATGTGT 70 22 0 40.91 58.025 0.00 0.00 0.00 3.975 84 TGTCTTGTGGGTGTGTCTGTTAA 61 23 0 43.48 61.000 0.00 0.00 0.00 4.000 85 TGTGTTGTGTATGGATGTAGCTTC 111 24 0 41.67 59.997 0.00 0.00 0.00 4.003 86 GATGTAGCTTCTTGACTGTGTG 124 22 0 45.45 57.988 0.00 0.00 0.00 4.012 87 CATGTGTGGCATTGTGTATACA 4 22 0 40.91 57.958 7.27 4.43 35.88 4.042 88 ATGTGTGGCATTGTGTATACAC 5 22 0 40.91 57.956 26.66 26.66 46.84 4.044 89 ACATGTGTGGCATTGTGTATAC 3 22 0 40.91 57.956 0.00 0.00 35.88 4.044 90 CATGTGTGTTGTGTATGGATGT 107 22 0 40.91 57.887 0.00 0.00 0.00 4.113 91 ACATGTGTGTTGTGTATGGATG 106 22 0 40.91 57.887 0.00 0.00 34.85 4.113 92 AACATGTGTGGCATTGTGTATACA 2 24 0 37.50 60.177 7.27 4.43 35.88 4.177 93 TGTGCATATACATGTGTGTTGTGT 97 24 0 37.50 60.177 3.23 0.00 40.30 4.177 94 GATGTAGCTTCTTGACTGTGTGTT 124 24 0 41.67 60.177 0.00 0.00 0.00 4.177 95 GTGTGTTGTGTATGGATGTAGCTT 110 24 0 41.67 60.237 0.00 0.00 0.00 4.237 96 GTGTTGTGTATGGATGTAGCTTCT 112 24 0 41.67 59.756 0.00 0.00 0.00 4.244 97 ATGTGTGTTGTGTATGGATGTAGC 108 24 0 41.67 60.297 0.00 0.00 0.00 4.297 98 TGTGCATATACATGTGTGTTGTG 97 23 0 39.13 58.624 3.23 0.00 40.30 4.376 99 GTGCATATACATGTGTGTTGTGT 98 23 0 39.13 58.623 3.23 0.00 40.30 4.377 100 AACATGTGTGGCATTGTGTATAC 2 23 0 39.13 58.615 0.00 0.00 35.88 4.385 101 GAACATGTGTGGCATTGTGTATAC 1 24 0 41.67 59.586 0.00 0.00 35.88 4.414 102 ATGTAGCTTCTTGACTGTGTGTTG 125 24 0 41.67 60.416 0.00 0.00 0.00 4.416 103 TGTTGTGTATGGATGTAGCTTCT 113 23 0 39.13 58.521 0.00 0.00 0.00 4.479 104 TGTGTATGGATGTAGCTTCTTGAC 116 24 0 41.67 59.515 0.00 0.00 0.00 4.485 105 TGTGTCTGTTAAACATGTGTGGTA 72 24 0 37.50 59.511 0.00 0.00 0.00 4.489 106 TAAACATGTGTGGTATTGTGCA 81 22 0 36.36 57.471 0.00 0.00 0.00 4.529 107 GTGTTGTGTATGGATGTAGCTTC 112 23 0 43.48 58.444 0.00 0.00 0.00 4.556 108 GTTAAACATGTGTGGTATTGTGCA 79 24 0 37.50 59.401 0.00 0.00 0.00 4.599 109 TGTTAAACATGTGTGGTATTGTGC 78 24 0 37.50 59.401 0.00 0.00 0.00 4.599 110 CTTGTGGGTGTGTCTGTTAAACAT 64 24 0 41.67 60.599 0.00 0.00 0.00 4.599 111 ACATGTGTGTTGTGTATGGATGTA 106 24 0 37.50 59.387 0.00 0.00 34.85 4.613 112 TACATGTGTGTTGTGTATGGATGT 105 24 0 37.50 59.387 9.61 0.00 40.30 4.613 113 GTGTCTTGTGGGTGTGTCTGTTA 60 23 0 47.83 61.614 0.00 0.00 0.00 4.614 114 GAACATGTGTGGCATTGTGTATA 1 23 0 39.13 58.364 0.00 0.00 35.88 4.636 115 GTGTTGTGTATGGATGTAGCTT 112 22 0 40.91 57.359 0.00 0.00 0.00 4.641 116 TGCATATACATGTGTGTTGTGT 99 22 0 36.36 57.281 3.23 0.00 40.30 4.719 117 TGTGCATATACATGTGTGTTGT 97 22 0 36.36 57.281 3.23 0.00 40.30 4.719 118 TATGGATGTAGCTTCTTGACTGTG 120 24 0 41.67 59.271 0.00 0.00 0.00 4.729 119 TGTATGGATGTAGCTTCTTGACTG 118 24 0 41.67 59.271 0.00 0.00 0.00 4.729 120 GTATGGATGTAGCTTCTTGACTGT 119 24 0 41.67 59.271 0.00 0.00 0.00 4.729 121 GTGTATGGATGTAGCTTCTTGACT 117 24 0 41.67 59.271 0.00 0.00 0.00 4.729 122 TGTGTATGGATGTAGCTTCTTGA 116 23 0 39.13 58.265 0.00 0.00 0.00 4.735 123 AACATGTGTGGTATTGTGCATA 83 22 0 36.36 57.263 0.00 0.00 0.00 4.737 124 AACATGTGTGGCATTGTGTATA 2 22 0 36.36 57.263 0.00 0.00 35.88 4.737 125 TTGTGCATATACATGTGTGTTGTG 96 24 0 37.50 59.225 3.23 0.00 40.30 4.775 126 ACATGTGTGGCATTGTGTATACAC 3 24 0 41.67 60.775 26.66 26.66 41.05 4.775 127 AGCTTCTTGACTGTGTGTTGTGT 129 23 0 43.48 61.808 0.00 0.00 0.00 4.808 128 GTGTGTCTGTTAAACATGTGTGGT 71 24 0 41.67 60.834 0.00 0.00 0.00 4.834 129 TTGTGGGTGTGTCTGTTAAACATG 65 24 0 41.67 60.837 0.00 0.00 0.00 4.837 130 TTAAACATGTGTGGTATTGTGCA 80 23 0 34.78 58.157 0.00 0.00 0.00 4.843 131 TGTTGTGTATGGATGTAGCTTCTT 113 24 0 37.50 59.140 0.00 0.00 0.00 4.860 132 TGTGTCTTGTGGGTGTGT 59 18 0 50.00 57.105 0.00 0.00 0.00 4.895 133 TGTTGTGTATGGATGTAGCTTC 113 22 0 40.91 57.096 0.00 0.00 0.00 4.904 134 ACATGTGTGGTATTGTGCATAT 84 22 0 36.36 57.054 0.00 0.00 0.00 4.946 135 TGTGTCTTGTGGGTGTGTCTGT 59 22 0 50.00 62.948 0.00 0.00 0.00 4.948 136 GTGTGTCTGTTAAACATGTGTG 71 22 0 40.91 57.042 0.00 0.00 0.00 4.958 137 TGTGTGTTGTGTATGGATGTAG 109 22 0 40.91 57.033 0.00 0.00 0.00 4.967 138 ATGGATGTAGCTTCTTGACTGTGT 121 24 0 41.67 60.971 0.00 0.00 0.00 4.971 139 TATGGATGTAGCTTCTTGACTGT 120 23 0 39.13 58.004 0.00 0.00 0.00 4.996 140 TGTATGGATGTAGCTTCTTGACT 118 23 0 39.13 58.004 0.00 0.00 0.00 4.996 141 TGTGCATATACATGTGTGTTGTGTA 97 25 0 36.00 60.005 3.23 0.00 40.30 5.005 142 TTGTGCATATACATGTGTGTTGT 96 23 0 34.78 57.973 3.23 0.00 40.30 5.027 143 AAACATGTGTGGTATTGTGCATA 82 23 0 34.78 57.961 0.00 0.00 0.00 5.039 144 TAAACATGTGTGGTATTGTGCAT 81 23 0 34.78 57.961 0.00 0.00 0.00 5.039 145 GTGTATGGATGTAGCTTCTTGAC 117 23 0 43.48 57.941 0.00 0.00 0.00 5.059 146 TTGTGTATGGATGTAGCTTCTTGAC 115 25 0 40.00 60.063 0.00 0.00 0.00 5.063 147 GTTGTGTATGGATGTAGCTTCTTGA 114 25 0 40.00 60.063 0.00 0.00 0.00 5.063 148 GTGTCTGTTAAACATGTGTGGTA 73 23 0 39.13 57.927 0.00 0.00 0.00 5.073 149 TTGTGTATGGATGTAGCTTCTTGA 115 24 0 37.50 58.896 0.00 0.00 0.00 5.104 150 TGTGTCTGTTAAACATGTGTGGTAT 72 25 0 36.00 59.887 0.00 0.00 0.00 5.113 151 GTCTTGTGGGTGTGTCTGTTAAAC 62 24 0 45.83 61.126 0.00 0.00 0.00 5.126 152 GTTAAACATGTGTGGTATTGTGC 79 23 0 39.13 57.839 0.00 0.00 0.00 5.161 153 TGGTATTGTGCATATACATGTGTGT 91 25 0 36.00 59.828 18.75 0.00 42.66 5.172 154 TGTGGTATTGTGCATATACATGTGT 89 25 0 36.00 59.828 8.22 0.00 34.94 5.172 155 TGTGTGGTATTGTGCATATACATGT 87 25 0 36.00 59.828 8.22 3.20 34.94 5.172 156 ACATGTGTGGTATTGTGCATATACA 84 25 0 36.00 59.828 18.75 7.54 0.00 5.172 157 GTAGCTTCTTGACTGTGTGTTGTG 127 24 0 45.83 61.177 0.00 0.00 0.00 5.177 158 CATGTGTGTTGTGTATGGATGTAG 107 24 0 41.67 58.810 0.00 0.00 0.00 5.190 159 GTTGTGTATGGATGTAGCTTCTTG 114 24 0 41.67 58.809 0.00 0.00 0.00 5.191 160 CATGTGTGTTGTGTATGGATGTA 107 23 0 39.13 57.795 0.00 0.00 0.00 5.205 161 TACATGTGTGTTGTGTATGGATG 105 23 0 39.13 57.795 9.61 0.00 40.30 5.205 162 TGTGTGTTGTGTATGGATGTAGCT 109 24 0 41.67 61.209 0.00 0.00 0.00 5.209 163 GTTAAACATGTGTGGTATTGTGCAT 79 25 0 36.00 59.776 0.00 0.00 0.00 5.224 164 ATACATGTGTGTTGTGTATGGATGT 104 25 0 36.00 59.769 9.61 0.00 38.35 5.231 165 AACATGTGTGGTATTGTGCATAT 83 23 0 34.78 57.763 0.00 0.00 0.00 5.237 166 CTGTTAAACATGTGTGGTATTGTGC 77 25 0 40.00 60.292 0.00 0.00 0.00 5.292 167 TGTTGTGTATGGATGTAGCTTCTTG 113 25 0 40.00 60.294 0.00 0.00 0.00 5.294 168 ACATGTGTGTTGTGTATGGATGTAG 106 25 0 40.00 60.294 0.00 0.00 34.85 5.294 169 GTGTTGTGTATGGATGTAGCTTCTT 112 25 0 40.00 60.294 0.00 0.00 0.00 5.294 170 GTATGGATGTAGCTTCTTGACTG 119 23 0 43.48 57.686 0.00 0.00 0.00 5.314 171 ATTGTGCATATACATGTGTGTTGTG 95 25 0 36.00 59.605 3.23 0.00 40.30 5.395 172 TTAAACATGTGTGGTATTGTGCAT 80 24 0 33.33 58.600 0.00 0.00 0.00 5.400 173 GTATGGATGTAGCTTCTTGACTGTG 119 25 0 44.00 60.408 0.00 0.00 0.00 5.408 174 GTGTATGGATGTAGCTTCTTGACTG 117 25 0 44.00 60.408 0.00 0.00 0.00 5.408 175 GGTGTGTCTGTTAAACATGTGTGG 70 24 0 45.83 61.422 0.00 0.00 0.00 5.422 176 GGGTGTGTCTGTTAAACATGTGTG 69 24 0 45.83 61.422 0.00 0.00 0.00 5.422 177 GTGGGTGTGTCTGTTAAACATGTG 67 24 0 45.83 61.422 0.00 0.00 0.00 5.422 178 TTGTGTATGGATGTAGCTTCTTG 115 23 0 39.13 57.536 0.00 0.00 0.00 5.464 179 ATGTGTGTTGTGTATGGATGTAG 108 23 0 39.13 57.536 0.00 0.00 0.00 5.464 180 GTTGTGTATGGATGTAGCTTCTT 114 23 0 39.13 57.535 0.00 0.00 0.00 5.465 181 GTGCATATACATGTGTGTTGTGTA 98 24 0 37.50 58.507 3.23 0.00 40.30 5.493 182 GCATATACATGTGTGTTGTGTATGG 100 25 0 40.00 59.494 17.56 6.84 44.43 5.506 183 GTGGTATTGTGCATATACATGTGTG 90 25 0 40.00 59.494 8.22 4.60 34.94 5.506 184 GTGTGGTATTGTGCATATACATGTG 88 25 0 40.00 59.494 9.61 0.00 34.94 5.506 185 TCTTGTGGGTGTGTCTGTTAAACA 63 24 0 41.67 61.514 0.00 0.00 0.00 5.514 186 TGTCTTGTGGGTGTGTCTGTTAAA 61 24 0 41.67 61.514 0.00 0.00 0.00 5.514 187 TAGCTTCTTGACTGTGTGTTGTGT 128 24 0 41.67 61.564 0.00 0.00 0.00 5.564 188 TGTAGCTTCTTGACTGTGTGTTGT 126 24 0 41.67 61.564 0.00 0.00 0.00 5.564 189 ATTGTGCATATACATGTGTGTTGT 95 24 0 33.33 58.422 3.23 0.00 40.30 5.578 190 AAACATGTGTGGTATTGTGCATAT 82 24 0 33.33 58.412 0.00 0.00 0.00 5.588 191 GTGTCTGTTAAACATGTGTGGTAT 73 24 0 37.50 58.375 0.00 0.00 0.00 5.625 192 GTGTGTCTGTTAAACATGTGTGGTA 71 25 0 40.00 60.639 0.00 0.00 0.00 5.639 193 TGGTATTGTGCATATACATGTGTG 91 24 0 37.50 58.313 18.75 4.60 34.94 5.687 194 TGTGGTATTGTGCATATACATGTG 89 24 0 37.50 58.313 9.61 0.00 34.94 5.687 195 TGTGTGGTATTGTGCATATACATG 87 24 0 37.50 58.313 8.22 0.00 35.35 5.687 196 CATGTGTGGTATTGTGCATATACA 85 24 0 37.50 58.313 18.75 7.54 0.00 5.687 197 GGTATTGTGCATATACATGTGTGT 92 24 0 37.50 58.312 18.75 0.00 42.66 5.688 198 GTGGTATTGTGCATATACATGTGT 90 24 0 37.50 58.312 8.22 0.00 34.94 5.688 199 GTGTGGTATTGTGCATATACATGT 88 24 0 37.50 58.312 8.22 3.20 34.94 5.688 200 ACATGTGTGGTATTGTGCATATAC 84 24 0 37.50 58.312 12.13 12.13 0.00 5.688 201 TTGTGCATATACATGTGTGTTGTGT 96 25 0 36.00 60.700 3.23 0.00 40.30 5.700 202 TGGATGTAGCTTCTTGACTGTGTG 122 24 0 45.83 61.738 0.00 0.00 0.00 5.738 203 GGATGTAGCTTCTTGACTGTGTGT 123 24 0 45.83 61.739 0.00 0.00 0.00 5.739 204 ATACATGTGTGTTGTGTATGGATG 104 24 0 37.50 58.249 9.61 0.00 38.35 5.751 205 TACATGTGTGTTGTGTATGGATGTA 105 25 0 36.00 59.243 9.61 0.00 40.30 5.757 206 TATGGATGTAGCTTCTTGACTGTGT 120 25 0 40.00 60.765 0.00 0.00 0.00 5.765 207 TGTATGGATGTAGCTTCTTGACTGT 118 25 0 40.00 60.765 0.00 0.00 0.00 5.765 208 TGTGTATGGATGTAGCTTCTTGACT 116 25 0 40.00 60.765 0.00 0.00 0.00 5.765 209 TGCATATACATGTGTGTTGTGTA 99 23 0 34.78 57.213 3.23 0.00 40.30 5.787 210 TGGGTGTGTCTGTTAAACATGTGT 68 24 0 41.67 61.817 0.00 0.00 0.00 5.817 211 TGTGGGTGTGTCTGTTAAACATGT 66 24 0 41.67 61.817 0.00 0.00 0.00 5.817 212 TGTTAAACATGTGTGGTATTGTGCA 78 25 0 36.00 60.875 0.00 0.00 0.00 5.875 213 TGTCTGTTAAACATGTGTGGTAT 74 23 0 34.78 57.063 0.00 0.00 0.00 5.937 214 GGTATTGTGCATATACATGTGTGTTG 92 26 0 38.46 60.015 18.75 0.00 40.30 6.015 215 GTGCATATACATGTGTGTTGTGTATG 98 26 0 38.46 60.016 13.84 13.84 45.64 6.016 216 TCTGTTAAACATGTGTGGTATTGTG 76 25 0 36.00 58.967 0.00 0.00 0.00 6.033 217 TGTCTGTTAAACATGTGTGGTATTG 74 25 0 36.00 58.967 0.00 0.00 0.00 6.033 218 GTCTGTTAAACATGTGTGGTATTGT 75 25 0 36.00 58.967 0.00 0.00 0.00 6.033 219 GTGTCTGTTAAACATGTGTGGTATT 73 25 0 36.00 58.967 0.00 0.00 0.00 6.033 220 GAACATGTGTGGCATTGTGTATACA 1 25 0 40.00 61.041 7.27 4.43 35.88 6.041 221 GTCTGTTAAACATGTGTGGTATTGTG 75 26 0 38.46 60.070 0.00 0.00 0.00 6.070 222 GTGTCTGTTAAACATGTGTGGTATTG 73 26 0 38.46 60.070 0.00 0.00 0.00 6.070 223 TGCATATACATGTGTGTTGTGTATG 99 25 0 36.00 58.914 13.84 13.84 45.64 6.086 224 GTGCATATACATGTGTGTTGTGTAT 98 25 0 36.00 58.914 4.13 3.79 41.36 6.086 225 GTGTGTTGTGTATGGATGTAGCTTC 110 25 0 44.00 61.090 0.00 0.00 0.00 6.090 226 GGTATTGTGCATATACATGTGTGTT 92 25 0 36.00 58.907 18.75 0.00 40.30 6.093 227 AACATGTGTGGTATTGTGCATATAC 83 25 0 36.00 58.907 12.13 12.13 0.00 6.093 228 GTGTCTTGTGGGTGTGTCTGTTAA 60 24 0 45.83 62.097 0.00 0.00 0.00 6.097 229 TACATGTGTGTTGTGTATGGATGTAG 105 26 0 38.46 60.124 9.61 0.00 40.30 6.124 230 TAAACATGTGTGGTATTGTGCATA 81 24 0 33.33 57.868 0.00 0.00 0.00 6.132 231 CATGTGTGGTATTGTGCATATACATG 85 26 0 38.46 59.850 13.99 9.60 35.35 6.150 232 ATGTGTGGTATTGTGCATATACATGT 86 26 0 34.62 60.180 8.22 3.20 34.94 6.180 233 ACATGTGTGGTATTGTGCATATACAT 84 26 0 34.62 60.180 8.22 0.00 0.00 6.180 234 TGTGTTGTGTATGGATGTAGCTTCT 111 25 0 40.00 61.231 0.00 0.00 0.00 6.231 235 GATGTAGCTTCTTGACTGTGTGTTG 124 25 0 44.00 61.257 0.00 0.00 0.00 6.257 236 TCTGTTAAACATGTGTGGTATTGT 76 24 0 33.33 57.742 0.00 0.00 0.00 6.258 237 TGTCTGTTAAACATGTGTGGTATT 74 24 0 33.33 57.742 0.00 0.00 0.00 6.258 238 ATGTGTGGTATTGTGCATATACATG 86 25 0 36.00 58.730 8.22 0.00 35.35 6.270 239 CATGTGTGGTATTGTGCATATACAT 85 25 0 36.00 58.730 8.22 0.00 0.00 6.270 240 AACATGTGTGGCATTGTGTATACAC 2 25 0 40.00 61.271 26.66 26.66 41.05 6.271 241 TGCATATACATGTGTGTTGTGTAT 99 24 0 33.33 57.692 3.79 3.79 41.36 6.308 242 CTGTTAAACATGTGTGGTATTGTG 77 24 0 37.50 57.686 0.00 0.00 0.00 6.314 243 AACATGTGTGGTATTGTGCATATA 83 24 0 33.33 57.678 0.00 0.00 0.00 6.322 244 TGTGCATATACATGTGTGTTGTGTAT 97 26 0 34.62 60.348 4.13 3.79 41.36 6.348 245 TGGTATTGTGCATATACATGTGTGTT 91 26 0 34.62 60.349 18.75 0.00 40.30 6.349 246 AACATGTGTGGTATTGTGCATATACA 83 26 0 34.62 60.349 18.75 7.54 0.00 6.349 247 CATGTGTGTTGTGTATGGATGTAGC 107 25 0 44.00 61.374 0.00 0.00 0.00 6.374 248 GTTAAACATGTGTGGTATTGTGCATA 79 26 0 34.62 59.626 0.00 0.00 0.00 6.374 249 ATACATGTGTGTTGTGTATGGATGTA 104 26 0 34.62 59.616 9.61 0.00 38.35 6.384 250 TATACATGTGTGTTGTGTATGGATGT 103 26 0 34.62 59.616 7.92 0.00 39.64 6.384 251 TGTCTGTTAAACATGTGTGGTATTGT 74 26 0 34.62 60.405 0.00 0.00 0.00 6.405 252 TGTGTCTGTTAAACATGTGTGGTATT 72 26 0 34.62 60.405 0.00 0.00 0.00 6.405 253 TTAAACATGTGTGGTATTGTGCATA 80 25 0 32.00 58.487 0.00 0.00 0.00 6.513 254 ATGTGTGGTATTGTGCATATACAT 86 24 0 33.33 57.486 8.22 0.00 0.00 6.514 255 TTGTGCATATACATGTGTGTTGTGTA 96 26 0 34.62 60.515 3.23 0.00 40.30 6.515 256 ATGTGTGTTGTGTATGGATGTAGCT 108 25 0 40.00 61.523 0.00 0.00 0.00 6.523 257 TATTGTGCATATACATGTGTGTTGTG 94 26 0 34.62 59.461 3.23 0.00 40.30 6.539 258 GTATTGTGCATATACATGTGTGTTGT 93 26 0 34.62 59.461 14.51 0.00 40.30 6.539 259 AAACATGTGTGGTATTGTGCATATAC 82 26 0 34.62 59.457 12.13 12.13 0.00 6.543 260 GTCTGTTAAACATGTGTGGTATTG 75 24 0 37.50 57.446 0.00 0.00 0.00 6.554 261 GCATATACATGTGTGTTGTGTATG 100 24 0 37.50 57.398 13.84 13.84 45.64 6.602 262 TGTGTTGTGTATAAATGTTGTGTCT 30 25 0 32.00 58.377 0.00 0.00 0.00 6.623 263 CTTGTGGGTGTGTCTGTTAAACATG 64 25 0 44.00 61.663 0.00 0.00 0.00 6.663 264 GCATATACATGTGTGTTGTGTATGGA 100 26 0 38.46 60.680 17.56 0.00 44.43 6.680 265 TATTGTGCATATACATGTGTGTTGT 94 25 0 32.00 58.316 3.23 0.00 40.30 6.684 266 AAACATGTGTGGTATTGTGCATATA 82 25 0 32.00 58.306 0.00 0.00 0.00 6.694 267 TAAACATGTGTGGTATTGTGCATAT 81 25 0 32.00 58.306 0.00 0.00 0.00 6.694 268 TGTGTGTTGTGTATGGATGTAGCTT 109 25 0 40.00 61.694 0.00 0.00 0.00 6.694 269 TCTTGTGGGTGTGTCTGTTAAACAT 63 25 0 40.00 61.817 0.00 0.00 0.00 6.817 270 AGCTTCTTGACTGTGTGTTGTGTG 129 24 0 45.83 62.850 0.00 0.00 0.00 6.850 271 TATACATGTGTGTTGTGTATGGATG 103 25 0 36.00 58.150 7.92 0.00 39.64 6.850 272 CATATACATGTGTGTTGTGTATGGA 101 25 0 36.00 58.150 11.52 0.00 42.41 6.850 273 ATGTAGCTTCTTGACTGTGTGTTGT 125 25 0 40.00 61.863 0.00 0.00 0.00 6.863 274 TGCATATACATGTGTGTTGTGTATGG 99 26 0 38.46 60.902 17.56 6.84 44.43 6.902 275 TGTGGTATTGTGCATATACATGTGTG 89 26 0 38.46 60.902 8.22 4.60 34.94 6.902 276 TGTGTGGTATTGTGCATATACATGTG 87 26 0 38.46 60.902 9.61 0.00 34.94 6.902 277 GTGGTATTGTGCATATACATGTGTGT 90 26 0 38.46 60.902 9.41 0.00 42.66 6.902 278 GTGTGGTATTGTGCATATACATGTGT 88 26 0 38.46 60.902 8.22 0.00 34.94 6.902 279 TGTGTTGTGTATAAATGTTGTGTC 30 24 0 33.33 57.075 0.00 0.00 0.00 6.925 280 GTGTGTCTGTTAAACATGTGTGGTAT 71 26 0 38.46 60.957 0.00 0.00 0.00 6.957 281 GTATTGTGCATATACATGTGTGTTG 93 25 0 36.00 58.019 14.51 0.00 40.30 6.981 282 ATTGTGCATATACATGTGTGTTGTGT 95 26 0 34.62 61.018 3.23 0.00 40.30 7.018 283 ATGGATGTAGCTTCTTGACTGTGTG 121 25 0 44.00 62.029 0.00 0.00 0.00 7.029 284 GTTAAACATGTGTGGTATTGTGCATAT 79 27 0 33.33 59.967 0.00 0.00 0.00 7.033 285 ATATACATGTGTGTTGTGTATGGATGT 102 27 0 33.33 59.963 7.92 0.00 39.64 7.037 286 TGTGTTGTGTATAAATGTTGTGTCTT 30 26 0 30.77 58.950 0.00 0.00 0.00 7.050 287 TTAAACATGTGTGGTATTGTGCATAT 80 26 0 30.77 58.886 0.00 0.00 0.00 7.114 288 GTTGTGTATGGATGTAGCTTCTTGAC 114 26 0 42.31 61.115 0.00 0.00 0.00 7.115 289 TGTTAAACATGTGTGGTATTGTGCAT 78 26 0 34.62 61.187 0.00 0.00 0.00 7.187 290 GTGTTGTGTATAAATGTTGTGTCTTCA 31 27 0 33.33 59.809 0.00 0.00 0.00 7.191 291 TGTGTTGTGTATAAATGTTGTGTCTTC 30 27 0 33.33 59.809 0.00 0.00 0.00 7.191 292 GGATGTAGCTTCTTGACTGTGTGTT 123 25 0 44.00 62.198 0.00 0.00 0.00 7.198 293 ATGTGTTGTGTATAAATGTTGTGTCT 29 26 0 30.77 58.778 0.00 0.00 0.00 7.222 294 TTGTGTATGGATGTAGCTTCTTGACT 115 26 0 38.46 61.252 0.00 0.00 0.00 7.252 295 TGTTGTGTATAAATGTTGTGTCTTCA 32 26 0 30.77 58.726 0.00 0.00 0.00 7.274 296 TTGTGGGTGTGTCTGTTAAACATGT 65 25 0 40.00 62.278 0.00 0.00 0.00 7.278 297 GTGTTGTGTATGGATGTAGCTTCTTG 112 26 0 42.31 61.334 0.00 0.00 0.00 7.334 298 CATATACATGTGTGTTGTGTATGGATG 101 27 0 37.04 59.649 11.52 3.24 42.41 7.351 299 ATATACATGTGTGTTGTGTATGGATG 102 26 0 34.62 58.558 7.92 0.00 39.64 7.442 300 CATATACATGTGTGTTGTGTATGGAT 101 26 0 34.62 58.558 11.52 0.00 42.41 7.442 301 ATACATGTGTGTTGTGTATGGATGTAG 104 27 0 37.04 60.450 9.61 0.00 38.35 7.450 302 TCTGTTAAACATGTGTGGTATTGTGC 76 26 0 38.46 61.452 0.00 0.00 0.00 7.452 303 ATGTGTTGTGTATAAATGTTGTGTC 29 25 0 32.00 57.534 0.00 0.00 0.00 7.466 304 TGTTGTGTATGGATGTAGCTTCTTGA 113 26 0 38.46 61.474 0.00 0.00 0.00 7.474 305 GTATTGTGCATATACATGTGTGTTGTG 93 27 0 37.04 60.497 14.51 0.00 40.30 7.497 306 GTGTTGTGTATAAATGTTGTGTCTT 31 25 0 32.00 57.477 0.00 0.00 0.00 7.523 307 TATACATGTGTGTTGTGTATGGATGTA 103 27 0 33.33 59.474 7.92 0.00 39.64 7.526 308 GTCTTGTGGGTGTGTCTGTTAAACA 62 25 0 44.00 62.541 0.00 0.00 0.00 7.541 309 TGTCTTGTGGGTGTGTCTGTTAAAC 61 25 0 44.00 62.541 0.00 0.00 0.00 7.541 310 GTGTCTTGTGGGTGTGTCTGTTAAA 60 25 0 44.00 62.541 0.00 0.00 0.00 7.541 311 GTGTTGTGTATAAATGTTGTGTCTTC 31 26 0 34.62 58.430 0.00 0.00 0.00 7.570 312 TAGCTTCTTGACTGTGTGTTGTGTG 128 25 0 44.00 62.579 0.00 0.00 0.00 7.579 313 TGTAGCTTCTTGACTGTGTGTTGTG 126 25 0 44.00 62.579 0.00 0.00 0.00 7.579 314 GTAGCTTCTTGACTGTGTGTTGTGT 127 25 0 44.00 62.581 0.00 0.00 0.00 7.581 315 ATATACATGTGTGTTGTGTATGGAT 102 25 0 32.00 57.351 7.92 0.00 39.64 7.649 316 AACATGTGTGGTATTGTGCATATACAT 83 27 0 33.33 60.670 8.22 0.00 0.00 7.670 317 CTGTTAAACATGTGTGGTATTGTGCA 77 26 0 38.46 61.672 0.00 0.00 0.00 7.672 318 TAAACATGTGTGGTATTGTGCATATAC 81 27 0 33.33 59.323 12.13 12.13 0.00 7.677 319 ATGTGTTGTGTATAAATGTTGTGTCTT 29 27 0 29.63 59.317 0.00 0.00 0.00 7.683 320 TGTGTTGTGTATGGATGTAGCTTCTT 111 26 0 38.46 61.698 0.00 0.00 0.00 7.698 321 GTTGTGTATAAATGTTGTGTCTTCA 33 25 0 32.00 57.246 0.00 0.00 0.00 7.754 322 TGTTGTGTATAAATGTTGTGTCTTC 32 25 0 32.00 57.246 0.00 0.00 0.00 7.754 323 TAAACATGTGTGGTATTGTGCATATA 81 26 0 30.77 58.208 0.00 0.00 0.00 7.792 324 TATGGATGTAGCTTCTTGACTGTGTG 120 26 0 42.31 61.796 0.00 0.00 0.00 7.796 325 TGTATGGATGTAGCTTCTTGACTGTG 118 26 0 42.31 61.796 0.00 0.00 0.00 7.796 326 TGTGTATGGATGTAGCTTCTTGACTG 116 26 0 42.31 61.796 0.00 0.00 0.00 7.796 327 GTATGGATGTAGCTTCTTGACTGTGT 119 26 0 42.31 61.797 0.00 0.00 0.00 7.797 328 GTGTATGGATGTAGCTTCTTGACTGT 117 26 0 42.31 61.797 0.00 0.00 0.00 7.797 329 TGGGTGTGTCTGTTAAACATGTGTG 68 25 0 44.00 62.827 0.00 0.00 0.00 7.827 330 TGTGGGTGTGTCTGTTAAACATGTG 66 25 0 44.00 62.827 0.00 0.00 0.00 7.827 331 TTGTGCATATACATGTGTGTTGTGTAT 96 27 0 33.33 60.828 4.13 3.79 41.36 7.828 332 ATTGTGCATATACATGTGTGTTGTGTA 95 27 0 33.33 60.828 3.23 0.00 40.30 7.828 333 TATTGTGCATATACATGTGTGTTGTGT 94 27 0 33.33 60.828 4.13 0.00 40.30 7.828 334 GGTGTGTCTGTTAAACATGTGTGGT 70 25 0 44.00 62.829 0.00 0.00 0.00 7.829 335 GTGGGTGTGTCTGTTAAACATGTGT 67 25 0 44.00 62.829 0.00 0.00 0.00 7.829 336 AAACATGTGTGGTATTGTGCATATACA 82 27 0 33.33 60.831 18.75 7.54 0.00 7.831 337 ATGTGTGTTGTGTATGGATGTAGCTT 108 26 0 38.46 61.979 0.00 0.00 0.00 7.979 338 GCATATACATGTGTGTTGTGTATGGAT 100 27 0 37.04 60.986 17.56 0.00 44.43 7.986 339 TGTTAAACATGTGTGGTATTGTGCATA 78 27 0 33.33 60.991 0.00 0.00 0.00 7.991 340 GAACATGTGTGGCATTGTGTATACAC 1 26 0 42.31 62.039 26.66 26.66 41.05 8.039 341 ATGTGTGGTATTGTGCATATACATGTG 86 27 0 37.04 61.200 9.61 0.00 34.94 8.200 342 CATGTGTGGTATTGTGCATATACATGT 85 27 0 37.04 61.200 15.63 3.20 34.94 8.200 343 ACATGTGTGGTATTGTGCATATACATG 84 27 0 37.04 61.200 17.52 16.48 36.76 8.200 344 TTAAACATGTGTGGTATTGTGCATATA 80 27 0 29.63 58.771 0.00 0.00 0.00 8.229 345 GTGTGTTGTGTATGGATGTAGCTTCT 110 26 0 42.31 62.236 0.00 0.00 0.00 8.236 346 TATGTGTTGTGTATAAATGTTGTGTCT 28 27 0 29.63 58.667 0.00 0.00 0.00 8.333 347 TGTGCATATACATGTGTGTTGTGTATG 97 27 0 37.04 61.352 13.84 13.84 45.64 8.352 348 TGGTATTGTGCATATACATGTGTGTTG 91 27 0 37.04 61.358 18.75 0.00 40.30 8.358 349 GGTATTGTGCATATACATGTGTGTTGT 92 27 0 37.04 61.358 18.75 0.00 40.30 8.358 350 GTGGTATTGTGCATATACATGTGTGTT 90 27 0 37.04 61.358 9.41 0.00 40.30 8.358 351 TGTCTGTTAAACATGTGTGGTATTGTG 74 27 0 37.04 61.410 0.00 0.00 0.00 8.410 352 TGTGTCTGTTAAACATGTGTGGTATTG 72 27 0 37.04 61.410 0.00 0.00 0.00 8.410 353 GTGTCTGTTAAACATGTGTGGTATTGT 73 27 0 37.04 61.410 0.00 0.00 0.00 8.410 354 GTGTGTCTGTTAAACATGTGTGGTATT 71 27 0 37.04 61.410 0.00 0.00 0.00 8.410 355 TGTGTGTTGTGTATGGATGTAGCTTC 109 26 0 42.31 62.452 0.00 0.00 0.00 8.452 356 CATGTGTGTTGTGTATGGATGTAGCT 107 26 0 42.31 62.512 0.00 0.00 0.00 8.512 357 TATGTGTTGTGTATAAATGTTGTGTC 28 26 0 30.77 57.465 0.00 0.00 0.00 8.535 358 GGTGTGTCTGTTAAACATGTGTGGTA 70 26 0 42.31 62.567 0.00 0.00 0.00 8.567 359 GATGTAGCTTCTTGACTGTGTGTTGT 124 26 0 42.31 62.610 0.00 0.00 0.00 8.610 360 ACATGTGTGTTGTGTATGGATGTAGC 106 26 0 42.31 62.728 0.00 0.00 34.85 8.728 361 TCTTGTGGGTGTGTCTGTTAAACATG 63 26 0 42.31 62.792 0.00 0.00 0.00 8.792 362 GTCTTGTGGGTGTGTCTGTTAAACAT 62 26 0 42.31 62.793 0.00 0.00 0.00 8.793 363 ATGTAGCTTCTTGACTGTGTGTTGTG 125 26 0 42.31 62.828 0.00 0.00 0.00 8.828 364 GTGCATATACATGTGTGTTGTGTATGG 98 27 0 40.74 61.873 17.56 6.84 44.43 8.873 365 GTGTGGTATTGTGCATATACATGTGTG 88 27 0 40.74 61.873 8.22 4.60 34.94 8.873 366 CATATGTGTTGTGTATAAATGTTGTGT 26 27 0 29.63 58.095 0.00 0.00 0.00 8.905 367 ACATATGTGTTGTGTATAAATGTTGTG 25 27 0 29.63 58.095 8.19 0.00 34.85 8.905 368 CACATATGTGTTGTGTATAAATGTTGT 24 27 0 29.63 58.095 25.59 0.00 41.45 8.905 369 CTGTTAAACATGTGTGGTATTGTGCAT 77 27 0 37.04 61.943 0.00 0.00 0.00 8.943 370 TGCATATACATGTGTGTTGTGTATGGA 99 27 0 37.04 62.020 17.56 14.16 44.43 9.020 371 ATATGTGTTGTGTATAAATGTTGTGTC 27 27 0 29.63 57.879 0.00 0.00 0.00 9.121 372 TTGTGTATGGATGTAGCTTCTTGACTG 115 27 0 40.74 62.221 0.00 0.00 0.00 9.221 373 GTTGTGTATGGATGTAGCTTCTTGACT 114 27 0 40.74 62.222 0.00 0.00 0.00 9.222 374 TGTGGTATTGTGCATATACATGTGTGT 89 27 0 37.04 62.236 9.41 0.00 42.66 9.236 375 TGTGTGGTATTGTGCATATACATGTGT 87 27 0 37.04 62.236 8.22 0.00 34.94 9.236 376 GTCTGTTAAACATGTGTGGTATTGTGC 75 27 0 40.74 62.394 0.00 0.00 0.00 9.394 377 TGTTGTGTATGGATGTAGCTTCTTGAC 113 27 0 40.74 62.430 0.00 0.00 0.00 9.430 378 GTGTTGTGTATGGATGTAGCTTCTTGA 112 27 0 40.74 62.430 0.00 0.00 0.00 9.430 379 TACATGTGTGTTGTGTATGGATGTAGC 105 27 0 40.74 62.480 9.61 0.00 40.30 9.480 380 TGTGTTGTGTATGGATGTAGCTTCTTG 111 27 0 40.74 62.643 0.00 0.00 0.00 9.643 381 GTGTGTTGTGTATGGATGTAGCTTCTT 110 27 0 40.74 62.644 0.00 0.00 0.00 9.644 382 ATGTGTGTTGTGTATGGATGTAGCTTC 108 27 0 40.74 62.698 0.00 0.00 0.00 9.698 383 GTATGGATGTAGCTTCTTGACTGTGTG 119 27 0 44.44 62.728 0.00 0.00 0.00 9.728 384 GTGTATGGATGTAGCTTCTTGACTGTG 117 27 0 44.44 62.728 0.00 0.00 0.00 9.728 385 TCTGTTAAACATGTGTGGTATTGTGCA 76 27 0 37.04 62.765 0.00 0.00 0.00 9.765 386 GGTGTGTCTGTTAAACATGTGTGGTAT 70 27 0 40.74 62.809 0.00 0.00 0.00 9.809 387 CATGTGTGTTGTGTATGGATGTAGCTT 107 27 0 40.74 62.910 0.00 0.00 0.00 9.910 primer3-2.3.6/test/primer1_th_list_last/AGAG.for0000664000077300007730000007065012231156243020402 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 AGAGAGAGAGAGAGAGAGAGAGA 55 23 0 47.83 58.820 0.00 0.00 0.00 4.180 1 AGAGAGAGAGAGAGAGAGAGAGA 53 23 0 47.83 58.820 0.00 0.00 0.00 4.180 2 AGAGAGAGAGAGAGAGAGAGAGA 51 23 0 47.83 58.820 0.00 0.00 0.00 4.180 3 AGAGAGAGAGAGAGAGAGAGAGA 49 23 0 47.83 58.820 0.00 0.00 0.00 4.180 4 AGAGAGAGAGAGAGAGAGAGAGA 47 23 0 47.83 58.820 0.00 0.00 0.00 4.180 5 AGAGAGAGAGAGAGAGAGAGAGA 45 23 0 47.83 58.820 0.00 0.00 0.00 4.180 6 AGAGAGAGAGAGAGAGAGAGAGA 43 23 0 47.83 58.820 0.00 0.00 0.00 4.180 7 AGAGAGAGAGAGAGAGAGAGAGA 41 23 0 47.83 58.820 0.00 0.00 0.00 4.180 8 AGAGAGAGAGAGAGAGAGAGAGA 39 23 0 47.83 58.820 0.00 0.00 0.00 4.180 9 AGAGAGAGAGAGAGAGAGAGAGA 37 23 0 47.83 58.820 0.00 0.00 0.00 4.180 10 AGAGAGAGAGAGAGAGAGAGAGA 35 23 0 47.83 58.820 0.00 0.00 0.00 4.180 11 AGAGAGAGAGAGAGAGAGAGAGA 33 23 0 47.83 58.820 0.00 0.00 0.00 4.180 12 AGAGAGAGAGAGAGAGAGAGAGA 31 23 0 47.83 58.820 0.00 0.00 0.00 4.180 13 AGAGAGAGAGAGAGAGAGAGAGA 29 23 0 47.83 58.820 0.00 0.00 0.00 4.180 14 AGAGAGAGAGAGAGAGAGAGAGA 27 23 0 47.83 58.820 0.00 0.00 0.00 4.180 15 AGAGAGAGAGAGAGAGAGAGAGA 25 23 0 47.83 58.820 0.00 0.00 0.00 4.180 16 AGAGAGAGAGAGAGAGAGAGAGA 23 23 0 47.83 58.820 0.00 0.00 0.00 4.180 17 AGAGAGAGAGAGAGAGAGAGAGA 21 23 0 47.83 58.820 0.00 0.00 0.00 4.180 18 AGAGAGAGAGAGAGAGAGAGAGA 19 23 0 47.83 58.820 0.00 0.00 0.00 4.180 19 AGAGAGAGAGAGAGAGAGAGAGA 17 23 0 47.83 58.820 0.00 0.00 0.00 4.180 20 AGAGAGAGAGAGAGAGAGAGAGA 15 23 0 47.83 58.820 0.00 0.00 0.00 4.180 21 AGAGAGAGAGAGAGAGAGAGAGA 13 23 0 47.83 58.820 0.00 0.00 0.00 4.180 22 AGAGAGAGAGAGAGAGAGAGAGA 11 23 0 47.83 58.820 0.00 0.00 0.00 4.180 23 AGAGAGAGAGAGAGAGAGAGAGA 9 23 0 47.83 58.820 0.00 0.00 0.00 4.180 24 AGAGAGAGAGAGAGAGAGAGAGA 7 23 0 47.83 58.820 0.00 0.00 0.00 4.180 25 AGAGAGAGAGAGAGAGAGAGAGA 5 23 0 47.83 58.820 0.00 0.00 0.00 4.180 26 AGAGAGAGAGAGAGAGAGAGAGA 3 23 0 47.83 58.820 0.00 0.00 0.00 4.180 27 AGAGAGAGAGAGAGAGAGAGAGA 1 23 0 47.83 58.820 0.00 0.00 0.00 4.180 28 GAGAGAGAGAGAGAGAGAGAGAGA 56 24 0 50.00 59.811 0.00 0.00 0.00 4.189 29 GAGAGAGAGAGAGAGAGAGAGAGA 54 24 0 50.00 59.811 0.00 0.00 0.00 4.189 30 GAGAGAGAGAGAGAGAGAGAGAGA 52 24 0 50.00 59.811 0.00 0.00 0.00 4.189 31 GAGAGAGAGAGAGAGAGAGAGAGA 50 24 0 50.00 59.811 0.00 0.00 0.00 4.189 32 GAGAGAGAGAGAGAGAGAGAGAGA 48 24 0 50.00 59.811 0.00 0.00 0.00 4.189 33 GAGAGAGAGAGAGAGAGAGAGAGA 46 24 0 50.00 59.811 0.00 0.00 0.00 4.189 34 GAGAGAGAGAGAGAGAGAGAGAGA 44 24 0 50.00 59.811 0.00 0.00 0.00 4.189 35 GAGAGAGAGAGAGAGAGAGAGAGA 42 24 0 50.00 59.811 0.00 0.00 0.00 4.189 36 GAGAGAGAGAGAGAGAGAGAGAGA 40 24 0 50.00 59.811 0.00 0.00 0.00 4.189 37 GAGAGAGAGAGAGAGAGAGAGAGA 38 24 0 50.00 59.811 0.00 0.00 0.00 4.189 38 GAGAGAGAGAGAGAGAGAGAGAGA 36 24 0 50.00 59.811 0.00 0.00 0.00 4.189 39 GAGAGAGAGAGAGAGAGAGAGAGA 34 24 0 50.00 59.811 0.00 0.00 0.00 4.189 40 GAGAGAGAGAGAGAGAGAGAGAGA 32 24 0 50.00 59.811 0.00 0.00 0.00 4.189 41 GAGAGAGAGAGAGAGAGAGAGAGA 30 24 0 50.00 59.811 0.00 0.00 0.00 4.189 42 GAGAGAGAGAGAGAGAGAGAGAGA 28 24 0 50.00 59.811 0.00 0.00 0.00 4.189 43 GAGAGAGAGAGAGAGAGAGAGAGA 26 24 0 50.00 59.811 0.00 0.00 0.00 4.189 44 GAGAGAGAGAGAGAGAGAGAGAGA 24 24 0 50.00 59.811 0.00 0.00 0.00 4.189 45 GAGAGAGAGAGAGAGAGAGAGAGA 22 24 0 50.00 59.811 0.00 0.00 0.00 4.189 46 GAGAGAGAGAGAGAGAGAGAGAGA 20 24 0 50.00 59.811 0.00 0.00 0.00 4.189 47 GAGAGAGAGAGAGAGAGAGAGAGA 18 24 0 50.00 59.811 0.00 0.00 0.00 4.189 48 GAGAGAGAGAGAGAGAGAGAGAGA 16 24 0 50.00 59.811 0.00 0.00 0.00 4.189 49 GAGAGAGAGAGAGAGAGAGAGAGA 14 24 0 50.00 59.811 0.00 0.00 0.00 4.189 50 GAGAGAGAGAGAGAGAGAGAGAGA 12 24 0 50.00 59.811 0.00 0.00 0.00 4.189 51 GAGAGAGAGAGAGAGAGAGAGAGA 10 24 0 50.00 59.811 0.00 0.00 0.00 4.189 52 GAGAGAGAGAGAGAGAGAGAGAGA 8 24 0 50.00 59.811 0.00 0.00 0.00 4.189 53 GAGAGAGAGAGAGAGAGAGAGAGA 6 24 0 50.00 59.811 0.00 0.00 0.00 4.189 54 GAGAGAGAGAGAGAGAGAGAGAGA 4 24 0 50.00 59.811 0.00 0.00 0.00 4.189 55 GAGAGAGAGAGAGAGAGAGAGAGA 2 24 0 50.00 59.811 0.00 0.00 0.00 4.189 56 AGAGAGAGAGAGAGAGAGAGAGAG 55 24 0 50.00 59.810 0.00 0.00 0.00 4.190 57 AGAGAGAGAGAGAGAGAGAGAGAG 53 24 0 50.00 59.810 0.00 0.00 0.00 4.190 58 AGAGAGAGAGAGAGAGAGAGAGAG 51 24 0 50.00 59.810 0.00 0.00 0.00 4.190 59 AGAGAGAGAGAGAGAGAGAGAGAG 49 24 0 50.00 59.810 0.00 0.00 0.00 4.190 60 AGAGAGAGAGAGAGAGAGAGAGAG 47 24 0 50.00 59.810 0.00 0.00 0.00 4.190 61 AGAGAGAGAGAGAGAGAGAGAGAG 45 24 0 50.00 59.810 0.00 0.00 0.00 4.190 62 AGAGAGAGAGAGAGAGAGAGAGAG 43 24 0 50.00 59.810 0.00 0.00 0.00 4.190 63 AGAGAGAGAGAGAGAGAGAGAGAG 41 24 0 50.00 59.810 0.00 0.00 0.00 4.190 64 AGAGAGAGAGAGAGAGAGAGAGAG 39 24 0 50.00 59.810 0.00 0.00 0.00 4.190 65 AGAGAGAGAGAGAGAGAGAGAGAG 37 24 0 50.00 59.810 0.00 0.00 0.00 4.190 66 AGAGAGAGAGAGAGAGAGAGAGAG 35 24 0 50.00 59.810 0.00 0.00 0.00 4.190 67 AGAGAGAGAGAGAGAGAGAGAGAG 33 24 0 50.00 59.810 0.00 0.00 0.00 4.190 68 AGAGAGAGAGAGAGAGAGAGAGAG 31 24 0 50.00 59.810 0.00 0.00 0.00 4.190 69 AGAGAGAGAGAGAGAGAGAGAGAG 29 24 0 50.00 59.810 0.00 0.00 0.00 4.190 70 AGAGAGAGAGAGAGAGAGAGAGAG 27 24 0 50.00 59.810 0.00 0.00 0.00 4.190 71 AGAGAGAGAGAGAGAGAGAGAGAG 25 24 0 50.00 59.810 0.00 0.00 0.00 4.190 72 AGAGAGAGAGAGAGAGAGAGAGAG 23 24 0 50.00 59.810 0.00 0.00 0.00 4.190 73 AGAGAGAGAGAGAGAGAGAGAGAG 21 24 0 50.00 59.810 0.00 0.00 0.00 4.190 74 AGAGAGAGAGAGAGAGAGAGAGAG 19 24 0 50.00 59.810 0.00 0.00 0.00 4.190 75 AGAGAGAGAGAGAGAGAGAGAGAG 17 24 0 50.00 59.810 0.00 0.00 0.00 4.190 76 AGAGAGAGAGAGAGAGAGAGAGAG 15 24 0 50.00 59.810 0.00 0.00 0.00 4.190 77 AGAGAGAGAGAGAGAGAGAGAGAG 13 24 0 50.00 59.810 0.00 0.00 0.00 4.190 78 AGAGAGAGAGAGAGAGAGAGAGAG 11 24 0 50.00 59.810 0.00 0.00 0.00 4.190 79 AGAGAGAGAGAGAGAGAGAGAGAG 9 24 0 50.00 59.810 0.00 0.00 0.00 4.190 80 AGAGAGAGAGAGAGAGAGAGAGAG 7 24 0 50.00 59.810 0.00 0.00 0.00 4.190 81 AGAGAGAGAGAGAGAGAGAGAGAG 5 24 0 50.00 59.810 0.00 0.00 0.00 4.190 82 AGAGAGAGAGAGAGAGAGAGAGAG 3 24 0 50.00 59.810 0.00 0.00 0.00 4.190 83 AGAGAGAGAGAGAGAGAGAGAGAG 1 24 0 50.00 59.810 0.00 0.00 0.00 4.190 84 GAGAGAGAGAGAGAGAGAGAGAG 56 23 0 52.17 58.478 0.00 0.00 0.00 4.522 85 GAGAGAGAGAGAGAGAGAGAGAG 54 23 0 52.17 58.478 0.00 0.00 0.00 4.522 86 GAGAGAGAGAGAGAGAGAGAGAG 52 23 0 52.17 58.478 0.00 0.00 0.00 4.522 87 GAGAGAGAGAGAGAGAGAGAGAG 50 23 0 52.17 58.478 0.00 0.00 0.00 4.522 88 GAGAGAGAGAGAGAGAGAGAGAG 48 23 0 52.17 58.478 0.00 0.00 0.00 4.522 89 GAGAGAGAGAGAGAGAGAGAGAG 46 23 0 52.17 58.478 0.00 0.00 0.00 4.522 90 GAGAGAGAGAGAGAGAGAGAGAG 44 23 0 52.17 58.478 0.00 0.00 0.00 4.522 91 GAGAGAGAGAGAGAGAGAGAGAG 42 23 0 52.17 58.478 0.00 0.00 0.00 4.522 92 GAGAGAGAGAGAGAGAGAGAGAG 40 23 0 52.17 58.478 0.00 0.00 0.00 4.522 93 GAGAGAGAGAGAGAGAGAGAGAG 38 23 0 52.17 58.478 0.00 0.00 0.00 4.522 94 GAGAGAGAGAGAGAGAGAGAGAG 36 23 0 52.17 58.478 0.00 0.00 0.00 4.522 95 GAGAGAGAGAGAGAGAGAGAGAG 34 23 0 52.17 58.478 0.00 0.00 0.00 4.522 96 GAGAGAGAGAGAGAGAGAGAGAG 32 23 0 52.17 58.478 0.00 0.00 0.00 4.522 97 GAGAGAGAGAGAGAGAGAGAGAG 30 23 0 52.17 58.478 0.00 0.00 0.00 4.522 98 GAGAGAGAGAGAGAGAGAGAGAG 28 23 0 52.17 58.478 0.00 0.00 0.00 4.522 99 GAGAGAGAGAGAGAGAGAGAGAG 26 23 0 52.17 58.478 0.00 0.00 0.00 4.522 100 GAGAGAGAGAGAGAGAGAGAGAG 24 23 0 52.17 58.478 0.00 0.00 0.00 4.522 101 GAGAGAGAGAGAGAGAGAGAGAG 22 23 0 52.17 58.478 0.00 0.00 0.00 4.522 102 GAGAGAGAGAGAGAGAGAGAGAG 20 23 0 52.17 58.478 0.00 0.00 0.00 4.522 103 GAGAGAGAGAGAGAGAGAGAGAG 18 23 0 52.17 58.478 0.00 0.00 0.00 4.522 104 GAGAGAGAGAGAGAGAGAGAGAG 16 23 0 52.17 58.478 0.00 0.00 0.00 4.522 105 GAGAGAGAGAGAGAGAGAGAGAG 14 23 0 52.17 58.478 0.00 0.00 0.00 4.522 106 GAGAGAGAGAGAGAGAGAGAGAG 12 23 0 52.17 58.478 0.00 0.00 0.00 4.522 107 GAGAGAGAGAGAGAGAGAGAGAG 10 23 0 52.17 58.478 0.00 0.00 0.00 4.522 108 GAGAGAGAGAGAGAGAGAGAGAG 8 23 0 52.17 58.478 0.00 0.00 0.00 4.522 109 GAGAGAGAGAGAGAGAGAGAGAG 6 23 0 52.17 58.478 0.00 0.00 0.00 4.522 110 GAGAGAGAGAGAGAGAGAGAGAG 4 23 0 52.17 58.478 0.00 0.00 0.00 4.522 111 GAGAGAGAGAGAGAGAGAGAGAG 2 23 0 52.17 58.478 0.00 0.00 0.00 4.522 112 GAGAGAGAGAGAGAGAGAGAGA 56 22 0 50.00 57.383 0.00 0.00 0.00 4.617 113 GAGAGAGAGAGAGAGAGAGAGA 54 22 0 50.00 57.383 0.00 0.00 0.00 4.617 114 GAGAGAGAGAGAGAGAGAGAGA 52 22 0 50.00 57.383 0.00 0.00 0.00 4.617 115 GAGAGAGAGAGAGAGAGAGAGA 50 22 0 50.00 57.383 0.00 0.00 0.00 4.617 116 GAGAGAGAGAGAGAGAGAGAGA 48 22 0 50.00 57.383 0.00 0.00 0.00 4.617 117 GAGAGAGAGAGAGAGAGAGAGA 46 22 0 50.00 57.383 0.00 0.00 0.00 4.617 118 GAGAGAGAGAGAGAGAGAGAGA 44 22 0 50.00 57.383 0.00 0.00 0.00 4.617 119 GAGAGAGAGAGAGAGAGAGAGA 42 22 0 50.00 57.383 0.00 0.00 0.00 4.617 120 GAGAGAGAGAGAGAGAGAGAGA 40 22 0 50.00 57.383 0.00 0.00 0.00 4.617 121 GAGAGAGAGAGAGAGAGAGAGA 38 22 0 50.00 57.383 0.00 0.00 0.00 4.617 122 GAGAGAGAGAGAGAGAGAGAGA 36 22 0 50.00 57.383 0.00 0.00 0.00 4.617 123 GAGAGAGAGAGAGAGAGAGAGA 34 22 0 50.00 57.383 0.00 0.00 0.00 4.617 124 GAGAGAGAGAGAGAGAGAGAGA 32 22 0 50.00 57.383 0.00 0.00 0.00 4.617 125 GAGAGAGAGAGAGAGAGAGAGA 30 22 0 50.00 57.383 0.00 0.00 0.00 4.617 126 GAGAGAGAGAGAGAGAGAGAGA 28 22 0 50.00 57.383 0.00 0.00 0.00 4.617 127 GAGAGAGAGAGAGAGAGAGAGA 26 22 0 50.00 57.383 0.00 0.00 0.00 4.617 128 GAGAGAGAGAGAGAGAGAGAGA 24 22 0 50.00 57.383 0.00 0.00 0.00 4.617 129 GAGAGAGAGAGAGAGAGAGAGA 22 22 0 50.00 57.383 0.00 0.00 0.00 4.617 130 GAGAGAGAGAGAGAGAGAGAGA 20 22 0 50.00 57.383 0.00 0.00 0.00 4.617 131 GAGAGAGAGAGAGAGAGAGAGA 18 22 0 50.00 57.383 0.00 0.00 0.00 4.617 132 GAGAGAGAGAGAGAGAGAGAGA 16 22 0 50.00 57.383 0.00 0.00 0.00 4.617 133 GAGAGAGAGAGAGAGAGAGAGA 14 22 0 50.00 57.383 0.00 0.00 0.00 4.617 134 GAGAGAGAGAGAGAGAGAGAGA 12 22 0 50.00 57.383 0.00 0.00 0.00 4.617 135 GAGAGAGAGAGAGAGAGAGAGA 10 22 0 50.00 57.383 0.00 0.00 0.00 4.617 136 GAGAGAGAGAGAGAGAGAGAGA 8 22 0 50.00 57.383 0.00 0.00 0.00 4.617 137 GAGAGAGAGAGAGAGAGAGAGA 6 22 0 50.00 57.383 0.00 0.00 0.00 4.617 138 GAGAGAGAGAGAGAGAGAGAGA 4 22 0 50.00 57.383 0.00 0.00 0.00 4.617 139 GAGAGAGAGAGAGAGAGAGAGA 2 22 0 50.00 57.383 0.00 0.00 0.00 4.617 140 AGAGAGAGAGAGAGAGAGAGAG 55 22 0 50.00 57.376 0.00 0.00 0.00 4.624 141 AGAGAGAGAGAGAGAGAGAGAG 53 22 0 50.00 57.376 0.00 0.00 0.00 4.624 142 AGAGAGAGAGAGAGAGAGAGAG 51 22 0 50.00 57.376 0.00 0.00 0.00 4.624 143 AGAGAGAGAGAGAGAGAGAGAG 49 22 0 50.00 57.376 0.00 0.00 0.00 4.624 144 AGAGAGAGAGAGAGAGAGAGAG 47 22 0 50.00 57.376 0.00 0.00 0.00 4.624 145 AGAGAGAGAGAGAGAGAGAGAG 45 22 0 50.00 57.376 0.00 0.00 0.00 4.624 146 AGAGAGAGAGAGAGAGAGAGAG 43 22 0 50.00 57.376 0.00 0.00 0.00 4.624 147 AGAGAGAGAGAGAGAGAGAGAG 41 22 0 50.00 57.376 0.00 0.00 0.00 4.624 148 AGAGAGAGAGAGAGAGAGAGAG 39 22 0 50.00 57.376 0.00 0.00 0.00 4.624 149 AGAGAGAGAGAGAGAGAGAGAG 37 22 0 50.00 57.376 0.00 0.00 0.00 4.624 150 AGAGAGAGAGAGAGAGAGAGAG 35 22 0 50.00 57.376 0.00 0.00 0.00 4.624 151 AGAGAGAGAGAGAGAGAGAGAG 33 22 0 50.00 57.376 0.00 0.00 0.00 4.624 152 AGAGAGAGAGAGAGAGAGAGAG 31 22 0 50.00 57.376 0.00 0.00 0.00 4.624 153 AGAGAGAGAGAGAGAGAGAGAG 29 22 0 50.00 57.376 0.00 0.00 0.00 4.624 154 AGAGAGAGAGAGAGAGAGAGAG 27 22 0 50.00 57.376 0.00 0.00 0.00 4.624 155 AGAGAGAGAGAGAGAGAGAGAG 25 22 0 50.00 57.376 0.00 0.00 0.00 4.624 156 AGAGAGAGAGAGAGAGAGAGAG 23 22 0 50.00 57.376 0.00 0.00 0.00 4.624 157 AGAGAGAGAGAGAGAGAGAGAG 21 22 0 50.00 57.376 0.00 0.00 0.00 4.624 158 AGAGAGAGAGAGAGAGAGAGAG 19 22 0 50.00 57.376 0.00 0.00 0.00 4.624 159 AGAGAGAGAGAGAGAGAGAGAG 17 22 0 50.00 57.376 0.00 0.00 0.00 4.624 160 AGAGAGAGAGAGAGAGAGAGAG 15 22 0 50.00 57.376 0.00 0.00 0.00 4.624 161 AGAGAGAGAGAGAGAGAGAGAG 13 22 0 50.00 57.376 0.00 0.00 0.00 4.624 162 AGAGAGAGAGAGAGAGAGAGAG 11 22 0 50.00 57.376 0.00 0.00 0.00 4.624 163 AGAGAGAGAGAGAGAGAGAGAG 9 22 0 50.00 57.376 0.00 0.00 0.00 4.624 164 AGAGAGAGAGAGAGAGAGAGAG 7 22 0 50.00 57.376 0.00 0.00 0.00 4.624 165 AGAGAGAGAGAGAGAGAGAGAG 5 22 0 50.00 57.376 0.00 0.00 0.00 4.624 166 AGAGAGAGAGAGAGAGAGAGAG 3 22 0 50.00 57.376 0.00 0.00 0.00 4.624 167 AGAGAGAGAGAGAGAGAGAGAG 1 22 0 50.00 57.376 0.00 0.00 0.00 4.624 168 GAGAGAGAGAGAGAGAGAGAGAGAG 56 25 0 52.00 60.701 0.00 0.00 0.00 5.701 169 GAGAGAGAGAGAGAGAGAGAGAGAG 54 25 0 52.00 60.701 0.00 0.00 0.00 5.701 170 GAGAGAGAGAGAGAGAGAGAGAGAG 52 25 0 52.00 60.701 0.00 0.00 0.00 5.701 171 GAGAGAGAGAGAGAGAGAGAGAGAG 50 25 0 52.00 60.701 0.00 0.00 0.00 5.701 172 GAGAGAGAGAGAGAGAGAGAGAGAG 48 25 0 52.00 60.701 0.00 0.00 0.00 5.701 173 GAGAGAGAGAGAGAGAGAGAGAGAG 46 25 0 52.00 60.701 0.00 0.00 0.00 5.701 174 GAGAGAGAGAGAGAGAGAGAGAGAG 44 25 0 52.00 60.701 0.00 0.00 0.00 5.701 175 GAGAGAGAGAGAGAGAGAGAGAGAG 42 25 0 52.00 60.701 0.00 0.00 0.00 5.701 176 GAGAGAGAGAGAGAGAGAGAGAGAG 40 25 0 52.00 60.701 0.00 0.00 0.00 5.701 177 GAGAGAGAGAGAGAGAGAGAGAGAG 38 25 0 52.00 60.701 0.00 0.00 0.00 5.701 178 GAGAGAGAGAGAGAGAGAGAGAGAG 36 25 0 52.00 60.701 0.00 0.00 0.00 5.701 179 GAGAGAGAGAGAGAGAGAGAGAGAG 34 25 0 52.00 60.701 0.00 0.00 0.00 5.701 180 GAGAGAGAGAGAGAGAGAGAGAGAG 32 25 0 52.00 60.701 0.00 0.00 0.00 5.701 181 GAGAGAGAGAGAGAGAGAGAGAGAG 30 25 0 52.00 60.701 0.00 0.00 0.00 5.701 182 GAGAGAGAGAGAGAGAGAGAGAGAG 28 25 0 52.00 60.701 0.00 0.00 0.00 5.701 183 GAGAGAGAGAGAGAGAGAGAGAGAG 26 25 0 52.00 60.701 0.00 0.00 0.00 5.701 184 GAGAGAGAGAGAGAGAGAGAGAGAG 24 25 0 52.00 60.701 0.00 0.00 0.00 5.701 185 GAGAGAGAGAGAGAGAGAGAGAGAG 22 25 0 52.00 60.701 0.00 0.00 0.00 5.701 186 GAGAGAGAGAGAGAGAGAGAGAGAG 20 25 0 52.00 60.701 0.00 0.00 0.00 5.701 187 GAGAGAGAGAGAGAGAGAGAGAGAG 18 25 0 52.00 60.701 0.00 0.00 0.00 5.701 188 GAGAGAGAGAGAGAGAGAGAGAGAG 16 25 0 52.00 60.701 0.00 0.00 0.00 5.701 189 GAGAGAGAGAGAGAGAGAGAGAGAG 14 25 0 52.00 60.701 0.00 0.00 0.00 5.701 190 GAGAGAGAGAGAGAGAGAGAGAGAG 12 25 0 52.00 60.701 0.00 0.00 0.00 5.701 191 GAGAGAGAGAGAGAGAGAGAGAGAG 10 25 0 52.00 60.701 0.00 0.00 0.00 5.701 192 GAGAGAGAGAGAGAGAGAGAGAGAG 8 25 0 52.00 60.701 0.00 0.00 0.00 5.701 193 GAGAGAGAGAGAGAGAGAGAGAGAG 6 25 0 52.00 60.701 0.00 0.00 0.00 5.701 194 GAGAGAGAGAGAGAGAGAGAGAGAG 4 25 0 52.00 60.701 0.00 0.00 0.00 5.701 195 GAGAGAGAGAGAGAGAGAGAGAGAG 2 25 0 52.00 60.701 0.00 0.00 0.00 5.701 196 AGAGAGAGAGAGAGAGAGAGAGAGA 55 25 0 48.00 61.070 0.00 0.00 0.00 6.070 197 AGAGAGAGAGAGAGAGAGAGAGAGA 53 25 0 48.00 61.070 0.00 0.00 0.00 6.070 198 AGAGAGAGAGAGAGAGAGAGAGAGA 51 25 0 48.00 61.070 0.00 0.00 0.00 6.070 199 AGAGAGAGAGAGAGAGAGAGAGAGA 49 25 0 48.00 61.070 0.00 0.00 0.00 6.070 200 AGAGAGAGAGAGAGAGAGAGAGAGA 47 25 0 48.00 61.070 0.00 0.00 0.00 6.070 201 AGAGAGAGAGAGAGAGAGAGAGAGA 45 25 0 48.00 61.070 0.00 0.00 0.00 6.070 202 AGAGAGAGAGAGAGAGAGAGAGAGA 43 25 0 48.00 61.070 0.00 0.00 0.00 6.070 203 AGAGAGAGAGAGAGAGAGAGAGAGA 41 25 0 48.00 61.070 0.00 0.00 0.00 6.070 204 AGAGAGAGAGAGAGAGAGAGAGAGA 39 25 0 48.00 61.070 0.00 0.00 0.00 6.070 205 AGAGAGAGAGAGAGAGAGAGAGAGA 37 25 0 48.00 61.070 0.00 0.00 0.00 6.070 206 AGAGAGAGAGAGAGAGAGAGAGAGA 35 25 0 48.00 61.070 0.00 0.00 0.00 6.070 207 AGAGAGAGAGAGAGAGAGAGAGAGA 33 25 0 48.00 61.070 0.00 0.00 0.00 6.070 208 AGAGAGAGAGAGAGAGAGAGAGAGA 31 25 0 48.00 61.070 0.00 0.00 0.00 6.070 209 AGAGAGAGAGAGAGAGAGAGAGAGA 29 25 0 48.00 61.070 0.00 0.00 0.00 6.070 210 AGAGAGAGAGAGAGAGAGAGAGAGA 27 25 0 48.00 61.070 0.00 0.00 0.00 6.070 211 AGAGAGAGAGAGAGAGAGAGAGAGA 25 25 0 48.00 61.070 0.00 0.00 0.00 6.070 212 AGAGAGAGAGAGAGAGAGAGAGAGA 23 25 0 48.00 61.070 0.00 0.00 0.00 6.070 213 AGAGAGAGAGAGAGAGAGAGAGAGA 21 25 0 48.00 61.070 0.00 0.00 0.00 6.070 214 AGAGAGAGAGAGAGAGAGAGAGAGA 19 25 0 48.00 61.070 0.00 0.00 0.00 6.070 215 AGAGAGAGAGAGAGAGAGAGAGAGA 17 25 0 48.00 61.070 0.00 0.00 0.00 6.070 216 AGAGAGAGAGAGAGAGAGAGAGAGA 15 25 0 48.00 61.070 0.00 0.00 0.00 6.070 217 AGAGAGAGAGAGAGAGAGAGAGAGA 13 25 0 48.00 61.070 0.00 0.00 0.00 6.070 218 AGAGAGAGAGAGAGAGAGAGAGAGA 11 25 0 48.00 61.070 0.00 0.00 0.00 6.070 219 AGAGAGAGAGAGAGAGAGAGAGAGA 9 25 0 48.00 61.070 0.00 0.00 0.00 6.070 220 AGAGAGAGAGAGAGAGAGAGAGAGA 7 25 0 48.00 61.070 0.00 0.00 0.00 6.070 221 AGAGAGAGAGAGAGAGAGAGAGAGA 5 25 0 48.00 61.070 0.00 0.00 0.00 6.070 222 AGAGAGAGAGAGAGAGAGAGAGAGA 3 25 0 48.00 61.070 0.00 0.00 0.00 6.070 223 AGAGAGAGAGAGAGAGAGAGAGAGA 1 25 0 48.00 61.070 0.00 0.00 0.00 6.070 224 GAGAGAGAGAGAGAGAGAGAGAGAGA 56 26 0 50.00 61.874 0.00 0.00 0.00 7.874 225 GAGAGAGAGAGAGAGAGAGAGAGAGA 54 26 0 50.00 61.874 0.00 0.00 0.00 7.874 226 GAGAGAGAGAGAGAGAGAGAGAGAGA 52 26 0 50.00 61.874 0.00 0.00 0.00 7.874 227 GAGAGAGAGAGAGAGAGAGAGAGAGA 50 26 0 50.00 61.874 0.00 0.00 0.00 7.874 228 GAGAGAGAGAGAGAGAGAGAGAGAGA 48 26 0 50.00 61.874 0.00 0.00 0.00 7.874 229 GAGAGAGAGAGAGAGAGAGAGAGAGA 46 26 0 50.00 61.874 0.00 0.00 0.00 7.874 230 GAGAGAGAGAGAGAGAGAGAGAGAGA 44 26 0 50.00 61.874 0.00 0.00 0.00 7.874 231 GAGAGAGAGAGAGAGAGAGAGAGAGA 42 26 0 50.00 61.874 0.00 0.00 0.00 7.874 232 GAGAGAGAGAGAGAGAGAGAGAGAGA 40 26 0 50.00 61.874 0.00 0.00 0.00 7.874 233 GAGAGAGAGAGAGAGAGAGAGAGAGA 38 26 0 50.00 61.874 0.00 0.00 0.00 7.874 234 GAGAGAGAGAGAGAGAGAGAGAGAGA 36 26 0 50.00 61.874 0.00 0.00 0.00 7.874 235 GAGAGAGAGAGAGAGAGAGAGAGAGA 34 26 0 50.00 61.874 0.00 0.00 0.00 7.874 236 GAGAGAGAGAGAGAGAGAGAGAGAGA 32 26 0 50.00 61.874 0.00 0.00 0.00 7.874 237 GAGAGAGAGAGAGAGAGAGAGAGAGA 30 26 0 50.00 61.874 0.00 0.00 0.00 7.874 238 GAGAGAGAGAGAGAGAGAGAGAGAGA 28 26 0 50.00 61.874 0.00 0.00 0.00 7.874 239 GAGAGAGAGAGAGAGAGAGAGAGAGA 26 26 0 50.00 61.874 0.00 0.00 0.00 7.874 240 GAGAGAGAGAGAGAGAGAGAGAGAGA 24 26 0 50.00 61.874 0.00 0.00 0.00 7.874 241 GAGAGAGAGAGAGAGAGAGAGAGAGA 22 26 0 50.00 61.874 0.00 0.00 0.00 7.874 242 GAGAGAGAGAGAGAGAGAGAGAGAGA 20 26 0 50.00 61.874 0.00 0.00 0.00 7.874 243 GAGAGAGAGAGAGAGAGAGAGAGAGA 18 26 0 50.00 61.874 0.00 0.00 0.00 7.874 244 GAGAGAGAGAGAGAGAGAGAGAGAGA 16 26 0 50.00 61.874 0.00 0.00 0.00 7.874 245 GAGAGAGAGAGAGAGAGAGAGAGAGA 14 26 0 50.00 61.874 0.00 0.00 0.00 7.874 246 GAGAGAGAGAGAGAGAGAGAGAGAGA 12 26 0 50.00 61.874 0.00 0.00 0.00 7.874 247 GAGAGAGAGAGAGAGAGAGAGAGAGA 10 26 0 50.00 61.874 0.00 0.00 0.00 7.874 248 GAGAGAGAGAGAGAGAGAGAGAGAGA 8 26 0 50.00 61.874 0.00 0.00 0.00 7.874 249 GAGAGAGAGAGAGAGAGAGAGAGAGA 6 26 0 50.00 61.874 0.00 0.00 0.00 7.874 250 GAGAGAGAGAGAGAGAGAGAGAGAGA 4 26 0 50.00 61.874 0.00 0.00 0.00 7.874 251 GAGAGAGAGAGAGAGAGAGAGAGAGA 2 26 0 50.00 61.874 0.00 0.00 0.00 7.874 252 AGAGAGAGAGAGAGAGAGAGAGAGAG 55 26 0 50.00 61.877 0.00 0.00 0.00 7.877 253 AGAGAGAGAGAGAGAGAGAGAGAGAG 53 26 0 50.00 61.877 0.00 0.00 0.00 7.877 254 AGAGAGAGAGAGAGAGAGAGAGAGAG 51 26 0 50.00 61.877 0.00 0.00 0.00 7.877 255 AGAGAGAGAGAGAGAGAGAGAGAGAG 49 26 0 50.00 61.877 0.00 0.00 0.00 7.877 256 AGAGAGAGAGAGAGAGAGAGAGAGAG 47 26 0 50.00 61.877 0.00 0.00 0.00 7.877 257 AGAGAGAGAGAGAGAGAGAGAGAGAG 45 26 0 50.00 61.877 0.00 0.00 0.00 7.877 258 AGAGAGAGAGAGAGAGAGAGAGAGAG 43 26 0 50.00 61.877 0.00 0.00 0.00 7.877 259 AGAGAGAGAGAGAGAGAGAGAGAGAG 41 26 0 50.00 61.877 0.00 0.00 0.00 7.877 260 AGAGAGAGAGAGAGAGAGAGAGAGAG 39 26 0 50.00 61.877 0.00 0.00 0.00 7.877 261 AGAGAGAGAGAGAGAGAGAGAGAGAG 37 26 0 50.00 61.877 0.00 0.00 0.00 7.877 262 AGAGAGAGAGAGAGAGAGAGAGAGAG 35 26 0 50.00 61.877 0.00 0.00 0.00 7.877 263 AGAGAGAGAGAGAGAGAGAGAGAGAG 33 26 0 50.00 61.877 0.00 0.00 0.00 7.877 264 AGAGAGAGAGAGAGAGAGAGAGAGAG 31 26 0 50.00 61.877 0.00 0.00 0.00 7.877 265 AGAGAGAGAGAGAGAGAGAGAGAGAG 29 26 0 50.00 61.877 0.00 0.00 0.00 7.877 266 AGAGAGAGAGAGAGAGAGAGAGAGAG 27 26 0 50.00 61.877 0.00 0.00 0.00 7.877 267 AGAGAGAGAGAGAGAGAGAGAGAGAG 25 26 0 50.00 61.877 0.00 0.00 0.00 7.877 268 AGAGAGAGAGAGAGAGAGAGAGAGAG 23 26 0 50.00 61.877 0.00 0.00 0.00 7.877 269 AGAGAGAGAGAGAGAGAGAGAGAGAG 21 26 0 50.00 61.877 0.00 0.00 0.00 7.877 270 AGAGAGAGAGAGAGAGAGAGAGAGAG 19 26 0 50.00 61.877 0.00 0.00 0.00 7.877 271 AGAGAGAGAGAGAGAGAGAGAGAGAG 17 26 0 50.00 61.877 0.00 0.00 0.00 7.877 272 AGAGAGAGAGAGAGAGAGAGAGAGAG 15 26 0 50.00 61.877 0.00 0.00 0.00 7.877 273 AGAGAGAGAGAGAGAGAGAGAGAGAG 13 26 0 50.00 61.877 0.00 0.00 0.00 7.877 274 AGAGAGAGAGAGAGAGAGAGAGAGAG 11 26 0 50.00 61.877 0.00 0.00 0.00 7.877 275 AGAGAGAGAGAGAGAGAGAGAGAGAG 9 26 0 50.00 61.877 0.00 0.00 0.00 7.877 276 AGAGAGAGAGAGAGAGAGAGAGAGAG 7 26 0 50.00 61.877 0.00 0.00 0.00 7.877 277 AGAGAGAGAGAGAGAGAGAGAGAGAG 5 26 0 50.00 61.877 0.00 0.00 0.00 7.877 278 AGAGAGAGAGAGAGAGAGAGAGAGAG 3 26 0 50.00 61.877 0.00 0.00 0.00 7.877 279 AGAGAGAGAGAGAGAGAGAGAGAGAG 1 26 0 50.00 61.877 0.00 0.00 0.00 7.877 280 GAGAGAGAGAGAGAGAGAGAGAGAGAG 56 27 0 51.85 62.605 0.00 0.00 0.00 9.605 281 GAGAGAGAGAGAGAGAGAGAGAGAGAG 54 27 0 51.85 62.605 0.00 0.00 0.00 9.605 282 GAGAGAGAGAGAGAGAGAGAGAGAGAG 52 27 0 51.85 62.605 0.00 0.00 0.00 9.605 283 GAGAGAGAGAGAGAGAGAGAGAGAGAG 50 27 0 51.85 62.605 0.00 0.00 0.00 9.605 284 GAGAGAGAGAGAGAGAGAGAGAGAGAG 48 27 0 51.85 62.605 0.00 0.00 0.00 9.605 285 GAGAGAGAGAGAGAGAGAGAGAGAGAG 46 27 0 51.85 62.605 0.00 0.00 0.00 9.605 286 GAGAGAGAGAGAGAGAGAGAGAGAGAG 44 27 0 51.85 62.605 0.00 0.00 0.00 9.605 287 GAGAGAGAGAGAGAGAGAGAGAGAGAG 42 27 0 51.85 62.605 0.00 0.00 0.00 9.605 288 GAGAGAGAGAGAGAGAGAGAGAGAGAG 40 27 0 51.85 62.605 0.00 0.00 0.00 9.605 289 GAGAGAGAGAGAGAGAGAGAGAGAGAG 38 27 0 51.85 62.605 0.00 0.00 0.00 9.605 290 GAGAGAGAGAGAGAGAGAGAGAGAGAG 36 27 0 51.85 62.605 0.00 0.00 0.00 9.605 291 GAGAGAGAGAGAGAGAGAGAGAGAGAG 34 27 0 51.85 62.605 0.00 0.00 0.00 9.605 292 GAGAGAGAGAGAGAGAGAGAGAGAGAG 32 27 0 51.85 62.605 0.00 0.00 0.00 9.605 293 GAGAGAGAGAGAGAGAGAGAGAGAGAG 30 27 0 51.85 62.605 0.00 0.00 0.00 9.605 294 GAGAGAGAGAGAGAGAGAGAGAGAGAG 28 27 0 51.85 62.605 0.00 0.00 0.00 9.605 295 GAGAGAGAGAGAGAGAGAGAGAGAGAG 26 27 0 51.85 62.605 0.00 0.00 0.00 9.605 296 GAGAGAGAGAGAGAGAGAGAGAGAGAG 24 27 0 51.85 62.605 0.00 0.00 0.00 9.605 297 GAGAGAGAGAGAGAGAGAGAGAGAGAG 22 27 0 51.85 62.605 0.00 0.00 0.00 9.605 298 GAGAGAGAGAGAGAGAGAGAGAGAGAG 20 27 0 51.85 62.605 0.00 0.00 0.00 9.605 299 GAGAGAGAGAGAGAGAGAGAGAGAGAG 18 27 0 51.85 62.605 0.00 0.00 0.00 9.605 300 GAGAGAGAGAGAGAGAGAGAGAGAGAG 16 27 0 51.85 62.605 0.00 0.00 0.00 9.605 301 GAGAGAGAGAGAGAGAGAGAGAGAGAG 14 27 0 51.85 62.605 0.00 0.00 0.00 9.605 302 GAGAGAGAGAGAGAGAGAGAGAGAGAG 12 27 0 51.85 62.605 0.00 0.00 0.00 9.605 303 GAGAGAGAGAGAGAGAGAGAGAGAGAG 10 27 0 51.85 62.605 0.00 0.00 0.00 9.605 304 GAGAGAGAGAGAGAGAGAGAGAGAGAG 8 27 0 51.85 62.605 0.00 0.00 0.00 9.605 305 GAGAGAGAGAGAGAGAGAGAGAGAGAG 6 27 0 51.85 62.605 0.00 0.00 0.00 9.605 306 GAGAGAGAGAGAGAGAGAGAGAGAGAG 4 27 0 51.85 62.605 0.00 0.00 0.00 9.605 307 GAGAGAGAGAGAGAGAGAGAGAGAGAG 2 27 0 51.85 62.605 0.00 0.00 0.00 9.605 308 AGAGAGAGAGAGAGAGAGAGAGAGAGA 55 27 0 48.15 62.991 0.00 0.00 0.00 9.991 309 AGAGAGAGAGAGAGAGAGAGAGAGAGA 53 27 0 48.15 62.991 0.00 0.00 0.00 9.991 310 AGAGAGAGAGAGAGAGAGAGAGAGAGA 51 27 0 48.15 62.991 0.00 0.00 0.00 9.991 311 AGAGAGAGAGAGAGAGAGAGAGAGAGA 49 27 0 48.15 62.991 0.00 0.00 0.00 9.991 312 AGAGAGAGAGAGAGAGAGAGAGAGAGA 47 27 0 48.15 62.991 0.00 0.00 0.00 9.991 313 AGAGAGAGAGAGAGAGAGAGAGAGAGA 45 27 0 48.15 62.991 0.00 0.00 0.00 9.991 314 AGAGAGAGAGAGAGAGAGAGAGAGAGA 43 27 0 48.15 62.991 0.00 0.00 0.00 9.991 315 AGAGAGAGAGAGAGAGAGAGAGAGAGA 41 27 0 48.15 62.991 0.00 0.00 0.00 9.991 316 AGAGAGAGAGAGAGAGAGAGAGAGAGA 39 27 0 48.15 62.991 0.00 0.00 0.00 9.991 317 AGAGAGAGAGAGAGAGAGAGAGAGAGA 37 27 0 48.15 62.991 0.00 0.00 0.00 9.991 318 AGAGAGAGAGAGAGAGAGAGAGAGAGA 35 27 0 48.15 62.991 0.00 0.00 0.00 9.991 319 AGAGAGAGAGAGAGAGAGAGAGAGAGA 33 27 0 48.15 62.991 0.00 0.00 0.00 9.991 320 AGAGAGAGAGAGAGAGAGAGAGAGAGA 31 27 0 48.15 62.991 0.00 0.00 0.00 9.991 321 AGAGAGAGAGAGAGAGAGAGAGAGAGA 29 27 0 48.15 62.991 0.00 0.00 0.00 9.991 322 AGAGAGAGAGAGAGAGAGAGAGAGAGA 27 27 0 48.15 62.991 0.00 0.00 0.00 9.991 323 AGAGAGAGAGAGAGAGAGAGAGAGAGA 25 27 0 48.15 62.991 0.00 0.00 0.00 9.991 324 AGAGAGAGAGAGAGAGAGAGAGAGAGA 23 27 0 48.15 62.991 0.00 0.00 0.00 9.991 325 AGAGAGAGAGAGAGAGAGAGAGAGAGA 21 27 0 48.15 62.991 0.00 0.00 0.00 9.991 326 AGAGAGAGAGAGAGAGAGAGAGAGAGA 19 27 0 48.15 62.991 0.00 0.00 0.00 9.991 327 AGAGAGAGAGAGAGAGAGAGAGAGAGA 17 27 0 48.15 62.991 0.00 0.00 0.00 9.991 328 AGAGAGAGAGAGAGAGAGAGAGAGAGA 15 27 0 48.15 62.991 0.00 0.00 0.00 9.991 329 AGAGAGAGAGAGAGAGAGAGAGAGAGA 13 27 0 48.15 62.991 0.00 0.00 0.00 9.991 330 AGAGAGAGAGAGAGAGAGAGAGAGAGA 11 27 0 48.15 62.991 0.00 0.00 0.00 9.991 331 AGAGAGAGAGAGAGAGAGAGAGAGAGA 9 27 0 48.15 62.991 0.00 0.00 0.00 9.991 332 AGAGAGAGAGAGAGAGAGAGAGAGAGA 7 27 0 48.15 62.991 0.00 0.00 0.00 9.991 333 AGAGAGAGAGAGAGAGAGAGAGAGAGA 5 27 0 48.15 62.991 0.00 0.00 0.00 9.991 334 AGAGAGAGAGAGAGAGAGAGAGAGAGA 3 27 0 48.15 62.991 0.00 0.00 0.00 9.991 335 AGAGAGAGAGAGAGAGAGAGAGAGAGA 1 27 0 48.15 62.991 0.00 0.00 0.00 9.991 primer3-2.3.6/test/primer1_th_list_last/SP01-03.txt.for0000664000077300007730000001625212231156243021422 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TGCAGGTTTAGAAGGGGTGT 66 20 0 50.00 59.801 0.00 0.00 0.00 0.199 1 CCTGCAGGTTTAGAAGGGGT 64 20 0 55.00 60.243 26.16 0.00 0.00 0.243 2 TTAGAAGGGGTGTCTGGGTG 73 20 0 55.00 59.583 0.00 0.00 0.00 0.417 3 ATCTGCACCCTGTAAACCCT 3 20 0 50.00 59.577 0.00 0.00 0.00 0.423 4 TCTGCACCCTGTAAACCCTG 4 20 0 55.00 60.533 0.00 0.00 0.00 0.533 5 GCAGGTTTAGAAGGGGTGTC 67 20 0 55.00 59.095 0.00 0.00 0.00 0.905 6 CTGCAGGTTTAGAAGGGGTG 65 20 0 55.00 59.093 6.10 0.00 0.00 0.907 7 CCTGTAAACCCTGGCTCCTG 11 20 0 60.00 60.964 0.00 0.00 0.00 0.964 8 CCCTGCAGGTTTAGAAGGGG 63 20 0 60.00 60.969 31.21 9.70 0.00 0.969 9 AGGTTTAGAAGGGGTGTCTGG 69 21 0 52.38 59.961 0.00 0.00 0.00 1.039 10 ACCCTGTAAACCCTGGCTC 9 19 0 57.89 59.860 0.00 0.00 0.00 1.140 11 CACCCTGTAAACCCTGGCT 8 19 0 57.89 60.168 0.00 0.00 0.00 1.168 12 TTTAGAAGGGGTGTCTGGGT 72 20 0 50.00 58.826 0.00 0.00 0.00 1.174 13 TTTAGAAGGGGTGTCTGGGTG 72 21 0 52.38 60.242 0.00 0.00 0.00 1.242 14 GTTTAGAAGGGGTGTCTGGGT 71 21 0 52.38 60.242 0.00 0.00 0.00 1.242 15 CACCCTGTAAACCCTGGCTC 8 20 0 60.00 61.249 0.00 0.00 0.00 1.249 16 CCTGTAAACCCTGGCTCCT 11 19 0 57.89 59.550 0.00 0.00 0.00 1.450 17 TGCACCCTGTAAACCCTGG 6 19 0 57.89 60.474 0.00 0.00 0.00 1.474 18 CTGCACCCTGTAAACCCTGG 5 20 0 60.00 61.536 0.00 0.00 0.00 1.536 19 TGTAAACCCTGGCTCCTGC 13 19 0 57.89 60.548 0.00 0.00 38.76 1.548 20 GTTTAGAAGGGGTGTCTGGG 71 20 0 55.00 58.433 0.00 0.00 0.00 1.567 21 GGTTTAGAAGGGGTGTCTGG 70 20 0 55.00 58.433 0.00 0.00 0.00 1.567 22 GCAGGTTTAGAAGGGGTGTCT 67 21 0 52.38 60.589 0.00 0.00 0.00 1.589 23 CTGTAAACCCTGGCTCCTGC 12 20 0 60.00 61.600 0.00 0.00 38.76 1.600 24 CCCTGTAAACCCTGGCTCC 10 19 0 63.16 60.625 0.00 0.00 0.00 1.625 25 TGCAGGTTTAGAAGGGGTGTC 66 21 0 52.38 60.864 0.00 0.00 0.00 1.864 26 CTGCAGGTTTAGAAGGGGTGT 65 21 0 52.38 60.866 6.10 0.00 0.00 1.866 27 CTGAGGTCAGCACACCCTG 49 19 0 63.16 60.916 0.00 0.00 37.75 1.916 28 TCTGCACCCTGTAAACCCT 4 19 0 52.63 59.079 0.00 0.00 0.00 1.921 29 GGTTTAGAAGGGGTGTCTGGG 70 21 0 57.14 60.934 0.00 0.00 0.00 1.934 30 ATCTGCACCCTGTAAACCCTG 3 21 0 52.38 60.936 0.00 0.00 0.00 1.936 31 CTGCACCCTGTAAACCCTG 5 19 0 57.89 58.965 0.00 0.00 0.00 2.035 32 CAGGTTTAGAAGGGGTGTCTG 68 21 0 52.38 58.866 0.00 0.00 0.00 2.134 33 TAGAAGGGGTGTCTGGGTGC 74 20 0 60.00 62.142 0.00 0.00 0.00 2.142 34 TAGAAGGGGTGTCTGGGTG 74 19 0 57.89 58.854 0.00 0.00 0.00 2.146 35 GGACACCCCACTGAGGTC 39 18 0 66.67 59.846 0.00 0.00 35.08 2.154 36 CCCTGTAAACCCTGGCTCCT 10 20 0 60.00 62.160 0.00 0.00 0.00 2.160 37 TGAGGTCAGCACACCCTG 50 18 0 61.11 59.766 0.00 0.00 37.75 2.234 38 ACCCTGTAAACCCTGGCTCC 9 20 0 60.00 62.448 0.00 0.00 0.00 2.448 39 CCTGCAGGTTTAGAAGGGGTG 64 21 0 57.14 61.548 26.16 0.00 0.00 2.548 40 CTGAGGTCAGCACACCCT 49 18 0 61.11 59.443 0.00 0.00 37.75 2.557 41 AGGTTTAGAAGGGGTGTCTG 69 20 0 50.00 57.384 0.00 0.00 0.00 2.616 42 CAGGTTTAGAAGGGGTGTCT 68 20 0 50.00 57.384 0.00 0.00 0.00 2.616 43 CTGTAAACCCTGGCTCCTG 12 19 0 57.89 58.355 0.00 0.00 0.00 2.645 44 CCTGCAGGTTTAGAAGGGG 64 19 0 57.89 58.343 26.16 0.00 0.00 2.657 45 CCCTGCAGGTTTAGAAGGG 63 19 0 57.89 58.343 31.21 10.50 0.00 2.657 46 GGACACCCCACTGAGGTCA 39 19 0 63.16 61.780 0.00 0.00 35.08 2.780 47 GAAGGGGTGTCTGGGTGC 76 18 0 66.67 60.880 0.00 0.00 0.00 2.880 48 GCACCCTGTAAACCCTGGC 7 19 0 63.16 61.895 0.00 0.00 0.00 2.895 49 TTAGAAGGGGTGTCTGGGT 73 19 0 52.63 58.044 0.00 0.00 0.00 2.956 50 GACACCCCACTGAGGTCA 40 18 0 61.11 59.037 0.00 0.00 35.08 2.963 51 AGAAGGGGTGTCTGGGTG 75 18 0 61.11 59.025 0.00 0.00 0.00 2.975 52 ATCTGCACCCTGTAAACCC 3 19 0 52.63 57.946 0.00 0.00 0.00 3.054 53 TGCAGGTTTAGAAGGGGTG 66 19 0 52.63 57.872 0.00 0.00 0.00 3.128 54 GCAGGTTTAGAAGGGGTGT 67 19 0 52.63 57.870 0.00 0.00 0.00 3.130 55 CAGGTTTAGAAGGGGTGTCTGG 68 22 0 54.55 61.229 0.00 0.00 0.00 3.229 56 ACCCTGTAAACCCTGGCT 9 18 0 55.56 58.594 0.00 0.00 0.00 3.406 57 GTAAACCCTGGCTCCTGC 14 18 0 61.11 58.583 0.00 0.00 38.76 3.417 58 CTGCAGGTTTAGAAGGGGT 65 19 0 52.63 57.556 6.10 0.00 0.00 3.444 59 AGAAGGGGTGTCTGGGTGC 75 19 0 63.16 62.469 0.00 0.00 0.00 3.469 60 GTTTAGAAGGGGTGTCTGGGTG 71 22 0 54.55 61.489 0.00 0.00 0.00 3.489 61 CACCCTGTAAACCCTGGC 8 18 0 61.11 58.494 0.00 0.00 0.00 3.506 62 GCACCCTGTAAACCCTGG 7 18 0 61.11 58.494 0.00 0.00 0.00 3.506 63 TTAGAAGGGGTGTCTGGGTGC 73 21 0 57.14 62.677 0.00 0.00 0.00 3.677 64 CCCTGCAGGTTTAGAAGGGGT 63 21 0 57.14 62.699 31.21 0.00 0.00 3.699 65 GAAGGGGTGTCTGGGTGCA 76 19 0 63.16 62.766 0.00 0.00 0.00 3.766 66 GCAGGTTTAGAAGGGGTGTCTG 67 22 0 54.55 61.808 0.00 0.00 0.00 3.808 67 CTGCAGGTTTAGAAGGGGTGTC 65 22 0 54.55 61.808 6.10 0.00 0.00 3.808 68 TCTGCACCCTGTAAACCCTGG 4 21 0 57.14 62.952 0.00 0.00 0.00 3.952 69 CTCCTGCCTCTCCGGGAC 25 18 0 72.22 62.066 0.00 0.00 36.14 4.066 70 CCTGTAAACCCTGGCTCC 11 18 0 61.11 57.847 0.00 0.00 0.00 4.153 71 CCCTGTAAACCCTGGCTC 10 18 0 61.11 57.847 0.00 0.00 0.00 4.153 72 TGCAGGTTTAGAAGGGGTGTCT 66 22 0 50.00 62.257 0.00 0.00 0.00 4.257 73 AGGTTTAGAAGGGGTGTCTGGG 69 22 0 54.55 62.326 0.00 0.00 0.00 4.326 74 TGCACCCTGTAAACCCTG 6 18 0 55.56 57.673 0.00 0.00 0.00 4.327 75 GGTTTAGAAGGGGTGTCTGGGT 70 22 0 54.55 62.588 0.00 0.00 0.00 4.588 76 TCTGCACCCTGTAAACCC 4 18 0 55.56 57.349 0.00 0.00 0.00 4.651 77 CTGCACCCTGTAAACCCT 5 18 0 55.56 57.340 0.00 0.00 0.00 4.660 78 CTGCCTCTCCGGGACACC 28 18 0 72.22 62.688 0.00 0.00 0.00 4.688 79 CCTGCCTCTCCGGGACAC 27 18 0 72.22 62.688 0.00 0.00 33.37 4.688 80 TAGAAGGGGTGTCTGGGT 74 18 0 55.56 57.173 0.00 0.00 0.00 4.827 81 TCCTGCCTCTCCGGGACA 26 18 0 66.67 62.937 0.00 0.00 36.14 4.937 82 TGTAAACCCTGGCTCCTG 13 18 0 55.56 57.016 0.00 0.00 0.00 4.984 primer3-2.3.6/test/primer1_th_list_last/included_test.for0000664000077300007730000010144012231156243022521 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TGTCTTGTGGGTGTGTCTGT 81 20 0 50.00 60.024 0.00 0.00 0.00 0.024 1 TGTGTCTTGTGGGTGTGTCT 79 20 0 50.00 60.024 0.00 0.00 0.00 0.024 2 GTGTCTTGTGGGTGTGTCTG 80 20 0 55.00 59.605 0.00 0.00 0.00 0.395 3 GTCTTGTGGGTGTGTCTGTT 82 20 0 50.00 58.879 0.00 0.00 0.00 1.121 4 TGTCTTGTGGGTGTGTCTGTT 81 21 0 47.62 60.657 0.00 0.00 0.00 1.657 5 GAACATGTGTGGCATTGTGT 21 20 0 45.00 58.108 0.00 0.00 35.88 1.892 6 AGCTTCTTGACTGTGTGTTGT 149 21 0 42.86 58.935 0.00 0.00 0.00 2.065 7 AGCTTCTTGACTGTGTGTTGTG 149 22 0 45.45 60.239 0.00 0.00 0.00 2.239 8 GTCTTGTGGGTGTGTCTGTTA 82 21 0 47.62 58.734 0.00 0.00 0.00 2.266 9 TGTGTCTTGTGGGTGTGTCTG 79 21 0 52.38 61.332 0.00 0.00 0.00 2.332 10 GTGTCTTGTGGGTGTGTCTGT 80 21 0 52.38 61.333 0.00 0.00 0.00 2.333 11 TGTGGGTGTGTCTGTTAAACA 86 21 0 42.86 58.579 0.00 0.00 0.00 2.421 12 TGTCTTGTGGGTGTGTCTGTTA 81 22 0 45.45 60.439 0.00 0.00 0.00 2.439 13 TGTGTCTTGTGGGTGTGTC 79 19 0 52.63 58.440 0.00 0.00 0.00 2.560 14 GTCTTGTGGGTGTGTCTGTTAA 82 22 0 45.45 59.389 0.00 0.00 0.00 2.611 15 TCTTGTGGGTGTGTCTGTTA 83 20 0 45.00 57.250 0.00 0.00 0.00 2.750 16 TTGTGGGTGTGTCTGTTAAACA 85 22 0 40.91 59.248 0.00 0.00 0.00 2.752 17 TGTCTTGTGGGTGTGTCTG 81 19 0 52.63 58.133 0.00 0.00 0.00 2.867 18 GTCTTGTGGGTGTGTCTGT 82 19 0 52.63 58.131 0.00 0.00 0.00 2.869 19 GTGTCTTGTGGGTGTGTCT 80 19 0 52.63 58.131 0.00 0.00 0.00 2.869 20 AGCTTCTTGACTGTGTGTTG 149 20 0 45.00 57.112 0.00 0.00 0.00 2.888 21 TGTAGCTTCTTGACTGTGTGT 146 21 0 42.86 58.106 0.00 0.00 0.00 2.894 22 TGGGTGTGTCTGTTAAACATGT 88 22 0 40.91 59.046 0.00 0.00 0.00 2.954 23 TGTGGGTGTGTCTGTTAAACAT 86 22 0 40.91 59.046 0.00 0.00 0.00 2.954 24 TCTTGTGGGTGTGTCTGTTAA 83 21 0 42.86 58.017 0.00 0.00 0.00 2.983 25 GAACATGTGTGGCATTGTGTA 21 21 0 42.86 57.999 0.00 0.00 35.88 3.001 26 TGGATGTAGCTTCTTGACTGTG 142 22 0 45.45 58.995 0.00 0.00 0.00 3.005 27 GGATGTAGCTTCTTGACTGTGT 143 22 0 45.45 58.995 0.00 0.00 0.00 3.005 28 TCTTGTGGGTGTGTCTGTTAAAC 83 23 0 43.48 59.988 0.00 0.00 0.00 3.012 29 GTCTTGTGGGTGTGTCTGTTAAA 82 23 0 43.48 59.988 0.00 0.00 0.00 3.012 30 GTAGCTTCTTGACTGTGTGTTGT 147 23 0 43.48 60.052 0.00 0.00 0.00 3.052 31 TAGCTTCTTGACTGTGTGTTGTG 148 23 0 43.48 60.052 0.00 0.00 0.00 3.052 32 TGTAGCTTCTTGACTGTGTGTTG 146 23 0 43.48 60.052 0.00 0.00 0.00 3.052 33 TGTGTGTTGTGTATGGATGTAGC 129 23 0 43.48 59.926 0.00 0.00 0.00 3.074 34 TAGCTTCTTGACTGTGTGTTGT 148 22 0 40.91 58.793 0.00 0.00 0.00 3.207 35 TGTAGCTTCTTGACTGTGTGTT 146 22 0 40.91 58.793 0.00 0.00 0.00 3.207 36 GGATGTAGCTTCTTGACTGTGTG 143 23 0 47.83 60.238 0.00 0.00 0.00 3.238 37 CTTGTGGGTGTGTCTGTTAAACA 84 23 0 43.48 60.238 0.00 0.00 0.00 3.238 38 TCTTGTGGGTGTGTCTGTTAAA 83 22 0 40.91 58.714 0.00 0.00 0.00 3.286 39 TGGGTGTGTCTGTTAAACATGTG 88 23 0 43.48 60.301 0.00 0.00 0.00 3.301 40 TGTGGGTGTGTCTGTTAAACATG 86 23 0 43.48 60.301 0.00 0.00 0.00 3.301 41 GGGTGTGTCTGTTAAACATGTGT 89 23 0 43.48 60.301 0.00 0.00 0.00 3.301 42 GTGGGTGTGTCTGTTAAACATGT 87 23 0 43.48 60.301 0.00 0.00 0.00 3.301 43 GGGTGTGTCTGTTAAACATGTG 89 22 0 45.45 58.689 0.00 0.00 0.00 3.311 44 GTGGGTGTGTCTGTTAAACATG 87 22 0 45.45 58.689 0.00 0.00 0.00 3.311 45 GTGTGTTGTGTATGGATGTAGCT 130 23 0 43.48 59.675 0.00 0.00 0.00 3.325 46 TGTGTCTGTTAAACATGTGTGGT 92 23 0 39.13 59.671 0.00 0.00 0.00 3.329 47 TTGTGGGTGTGTCTGTTAAACAT 85 23 0 39.13 59.668 0.00 0.00 0.00 3.332 48 CTTGTGGGTGTGTCTGTTAAAC 84 22 0 45.45 58.625 0.00 0.00 0.00 3.375 49 GATGTAGCTTCTTGACTGTGTGT 144 23 0 43.48 59.615 0.00 0.00 0.00 3.385 50 ACATGTGTGGCATTGTGTATACA 23 23 0 39.13 59.607 7.27 4.43 35.88 3.393 51 TGGATGTAGCTTCTTGACTGT 142 21 0 42.86 57.597 0.00 0.00 0.00 3.403 52 ATGTAGCTTCTTGACTGTGTGT 145 22 0 40.91 58.590 0.00 0.00 0.00 3.410 53 AAACATGTGTGGTATTGTGCA 102 21 0 38.10 57.554 0.00 0.00 0.00 3.446 54 ACATGTGTGTTGTGTATGGATGT 126 23 0 39.13 59.542 0.00 0.00 34.85 3.458 55 TTGTGGGTGTGTCTGTTAAAC 85 21 0 42.86 57.507 0.00 0.00 0.00 3.493 56 GAACATGTGTGGCATTGTGTAT 21 22 0 40.91 58.480 0.00 0.00 35.88 3.520 57 TGTGTGGCATTGTGTATACAC 26 21 0 42.86 57.451 26.66 26.66 46.84 3.549 58 GTAGCTTCTTGACTGTGTGTTG 147 22 0 45.45 58.445 0.00 0.00 0.00 3.555 59 ATGGATGTAGCTTCTTGACTGTG 141 23 0 43.48 59.421 0.00 0.00 0.00 3.579 60 TGTGTTGTGTATGGATGTAGCT 131 22 0 40.91 58.384 0.00 0.00 0.00 3.616 61 TGGATGTAGCTTCTTGACTGTGT 142 23 0 43.48 60.620 0.00 0.00 0.00 3.620 62 AACATGTGTGGTATTGTGCAT 103 21 0 38.10 57.337 0.00 0.00 0.00 3.663 63 AACATGTGTGGCATTGTGTAT 22 21 0 38.10 57.337 0.00 0.00 35.88 3.663 64 TCTTGTGGGTGTGTCTGTT 83 19 0 47.37 57.331 0.00 0.00 0.00 3.669 65 GTGTGTCTGTTAAACATGTGTGG 91 23 0 43.48 59.315 0.00 0.00 0.00 3.685 66 GGTGTGTCTGTTAAACATGTGTG 90 23 0 43.48 59.315 0.00 0.00 0.00 3.685 67 GTGTGTTGTGTATGGATGTAGC 130 22 0 45.45 58.308 0.00 0.00 0.00 3.692 68 TGGGTGTGTCTGTTAAACATG 88 21 0 42.86 57.293 0.00 0.00 0.00 3.707 69 GGGTGTGTCTGTTAAACATGT 89 21 0 42.86 57.292 0.00 0.00 0.00 3.708 70 GTGGGTGTGTCTGTTAAACAT 87 21 0 42.86 57.292 0.00 0.00 0.00 3.708 71 GGATGTAGCTTCTTGACTGTG 143 21 0 47.62 57.263 0.00 0.00 0.00 3.737 72 CATGTGTGGCATTGTGTATACAC 24 23 0 43.48 59.252 26.66 26.66 41.05 3.748 73 ATGTAGCTTCTTGACTGTGTGTT 145 23 0 39.13 59.228 0.00 0.00 0.00 3.772 74 CTTGTGGGTGTGTCTGTTAAA 84 21 0 42.86 57.225 0.00 0.00 0.00 3.775 75 GTGTCTTGTGGGTGTGTCTGTT 80 22 0 50.00 61.870 0.00 0.00 0.00 3.870 76 ATGGATGTAGCTTCTTGACTGT 141 22 0 40.91 58.107 0.00 0.00 0.00 3.893 77 AAACATGTGTGGTATTGTGCAT 102 22 0 36.36 58.061 0.00 0.00 0.00 3.939 78 TAGCTTCTTGACTGTGTGTTG 148 21 0 42.86 57.047 0.00 0.00 0.00 3.953 79 GTAGCTTCTTGACTGTGTGTT 147 21 0 42.86 57.045 0.00 0.00 0.00 3.955 80 TGTGTTGTGTATGGATGTAGCTT 131 23 0 39.13 59.034 0.00 0.00 0.00 3.966 81 TGTGTCTGTTAAACATGTGTGG 92 22 0 40.91 58.026 0.00 0.00 0.00 3.974 82 GTGTCTGTTAAACATGTGTGGT 93 22 0 40.91 58.025 0.00 0.00 0.00 3.975 83 GGTGTGTCTGTTAAACATGTGT 90 22 0 40.91 58.025 0.00 0.00 0.00 3.975 84 TGTCTTGTGGGTGTGTCTGTTAA 81 23 0 43.48 61.000 0.00 0.00 0.00 4.000 85 TGTGTTGTGTATGGATGTAGCTTC 131 24 0 41.67 59.997 0.00 0.00 0.00 4.003 86 GATGTAGCTTCTTGACTGTGTG 144 22 0 45.45 57.988 0.00 0.00 0.00 4.012 87 CATGTGTGGCATTGTGTATACA 24 22 0 40.91 57.958 7.27 4.43 35.88 4.042 88 ATGTGTGGCATTGTGTATACAC 25 22 0 40.91 57.956 26.66 26.66 46.84 4.044 89 ACATGTGTGGCATTGTGTATAC 23 22 0 40.91 57.956 0.00 0.00 35.88 4.044 90 CATGTGTGTTGTGTATGGATGT 127 22 0 40.91 57.887 0.00 0.00 0.00 4.113 91 ACATGTGTGTTGTGTATGGATG 126 22 0 40.91 57.887 0.00 0.00 34.85 4.113 92 AACATGTGTGGCATTGTGTATACA 22 24 0 37.50 60.177 7.27 4.43 35.88 4.177 93 TGTGCATATACATGTGTGTTGTGT 117 24 0 37.50 60.177 3.23 0.00 40.30 4.177 94 GATGTAGCTTCTTGACTGTGTGTT 144 24 0 41.67 60.177 0.00 0.00 0.00 4.177 95 GTGTGTTGTGTATGGATGTAGCTT 130 24 0 41.67 60.237 0.00 0.00 0.00 4.237 96 GTGTTGTGTATGGATGTAGCTTCT 132 24 0 41.67 59.756 0.00 0.00 0.00 4.244 97 ATGTGTGTTGTGTATGGATGTAGC 128 24 0 41.67 60.297 0.00 0.00 0.00 4.297 98 TGTGCATATACATGTGTGTTGTG 117 23 0 39.13 58.624 3.23 0.00 40.30 4.376 99 GTGCATATACATGTGTGTTGTGT 118 23 0 39.13 58.623 3.23 0.00 40.30 4.377 100 AACATGTGTGGCATTGTGTATAC 22 23 0 39.13 58.615 0.00 0.00 35.88 4.385 101 GAACATGTGTGGCATTGTGTATAC 21 24 0 41.67 59.586 0.00 0.00 35.88 4.414 102 ATGTAGCTTCTTGACTGTGTGTTG 145 24 0 41.67 60.416 0.00 0.00 0.00 4.416 103 TGTTGTGTATGGATGTAGCTTCT 133 23 0 39.13 58.521 0.00 0.00 0.00 4.479 104 TGTGTATGGATGTAGCTTCTTGAC 136 24 0 41.67 59.515 0.00 0.00 0.00 4.485 105 TGTGTCTGTTAAACATGTGTGGTA 92 24 0 37.50 59.511 0.00 0.00 0.00 4.489 106 TAAACATGTGTGGTATTGTGCA 101 22 0 36.36 57.471 0.00 0.00 0.00 4.529 107 GTGTTGTGTATGGATGTAGCTTC 132 23 0 43.48 58.444 0.00 0.00 0.00 4.556 108 GTTAAACATGTGTGGTATTGTGCA 99 24 0 37.50 59.401 0.00 0.00 0.00 4.599 109 TGTTAAACATGTGTGGTATTGTGC 98 24 0 37.50 59.401 0.00 0.00 0.00 4.599 110 CTTGTGGGTGTGTCTGTTAAACAT 84 24 0 41.67 60.599 0.00 0.00 0.00 4.599 111 ACATGTGTGTTGTGTATGGATGTA 126 24 0 37.50 59.387 0.00 0.00 34.85 4.613 112 TACATGTGTGTTGTGTATGGATGT 125 24 0 37.50 59.387 9.61 0.00 40.30 4.613 113 GTGTCTTGTGGGTGTGTCTGTTA 80 23 0 47.83 61.614 0.00 0.00 0.00 4.614 114 GAACATGTGTGGCATTGTGTATA 21 23 0 39.13 58.364 0.00 0.00 35.88 4.636 115 GTGTTGTGTATGGATGTAGCTT 132 22 0 40.91 57.359 0.00 0.00 0.00 4.641 116 TGCATATACATGTGTGTTGTGT 119 22 0 36.36 57.281 3.23 0.00 40.30 4.719 117 TGTGCATATACATGTGTGTTGT 117 22 0 36.36 57.281 3.23 0.00 40.30 4.719 118 TATGGATGTAGCTTCTTGACTGTG 140 24 0 41.67 59.271 0.00 0.00 0.00 4.729 119 TGTATGGATGTAGCTTCTTGACTG 138 24 0 41.67 59.271 0.00 0.00 0.00 4.729 120 GTATGGATGTAGCTTCTTGACTGT 139 24 0 41.67 59.271 0.00 0.00 0.00 4.729 121 GTGTATGGATGTAGCTTCTTGACT 137 24 0 41.67 59.271 0.00 0.00 0.00 4.729 122 TGTGTATGGATGTAGCTTCTTGA 136 23 0 39.13 58.265 0.00 0.00 0.00 4.735 123 AACATGTGTGGTATTGTGCATA 103 22 0 36.36 57.263 0.00 0.00 0.00 4.737 124 AACATGTGTGGCATTGTGTATA 22 22 0 36.36 57.263 0.00 0.00 35.88 4.737 125 TTGTGCATATACATGTGTGTTGTG 116 24 0 37.50 59.225 3.23 0.00 40.30 4.775 126 ACATGTGTGGCATTGTGTATACAC 23 24 0 41.67 60.775 26.66 26.66 41.05 4.775 127 AGCTTCTTGACTGTGTGTTGTGT 149 23 0 43.48 61.808 0.00 0.00 0.00 4.808 128 GTGTGTCTGTTAAACATGTGTGGT 91 24 0 41.67 60.834 0.00 0.00 0.00 4.834 129 TTGTGGGTGTGTCTGTTAAACATG 85 24 0 41.67 60.837 0.00 0.00 0.00 4.837 130 TTAAACATGTGTGGTATTGTGCA 100 23 0 34.78 58.157 0.00 0.00 0.00 4.843 131 TGTTGTGTATGGATGTAGCTTCTT 133 24 0 37.50 59.140 0.00 0.00 0.00 4.860 132 TGTGTCTTGTGGGTGTGT 79 18 0 50.00 57.105 0.00 0.00 0.00 4.895 133 TGTTGTGTATGGATGTAGCTTC 133 22 0 40.91 57.096 0.00 0.00 0.00 4.904 134 ACATGTGTGGTATTGTGCATAT 104 22 0 36.36 57.054 0.00 0.00 0.00 4.946 135 TGTGTCTTGTGGGTGTGTCTGT 79 22 0 50.00 62.948 0.00 0.00 0.00 4.948 136 GTGTGTCTGTTAAACATGTGTG 91 22 0 40.91 57.042 0.00 0.00 0.00 4.958 137 TGTGTGTTGTGTATGGATGTAG 129 22 0 40.91 57.033 0.00 0.00 0.00 4.967 138 ATGGATGTAGCTTCTTGACTGTGT 141 24 0 41.67 60.971 0.00 0.00 0.00 4.971 139 TATGGATGTAGCTTCTTGACTGT 140 23 0 39.13 58.004 0.00 0.00 0.00 4.996 140 TGTATGGATGTAGCTTCTTGACT 138 23 0 39.13 58.004 0.00 0.00 0.00 4.996 141 TGTGCATATACATGTGTGTTGTGTA 117 25 0 36.00 60.005 3.23 0.00 40.30 5.005 142 TTGTGCATATACATGTGTGTTGT 116 23 0 34.78 57.973 3.23 0.00 40.30 5.027 143 AAACATGTGTGGTATTGTGCATA 102 23 0 34.78 57.961 0.00 0.00 0.00 5.039 144 TAAACATGTGTGGTATTGTGCAT 101 23 0 34.78 57.961 0.00 0.00 0.00 5.039 145 GTGTATGGATGTAGCTTCTTGAC 137 23 0 43.48 57.941 0.00 0.00 0.00 5.059 146 TTGTGTATGGATGTAGCTTCTTGAC 135 25 0 40.00 60.063 0.00 0.00 0.00 5.063 147 GTTGTGTATGGATGTAGCTTCTTGA 134 25 0 40.00 60.063 0.00 0.00 0.00 5.063 148 GTGTCTGTTAAACATGTGTGGTA 93 23 0 39.13 57.927 0.00 0.00 0.00 5.073 149 TTGTGTATGGATGTAGCTTCTTGA 135 24 0 37.50 58.896 0.00 0.00 0.00 5.104 150 TGTGTCTGTTAAACATGTGTGGTAT 92 25 0 36.00 59.887 0.00 0.00 0.00 5.113 151 GTCTTGTGGGTGTGTCTGTTAAAC 82 24 0 45.83 61.126 0.00 0.00 0.00 5.126 152 GTTAAACATGTGTGGTATTGTGC 99 23 0 39.13 57.839 0.00 0.00 0.00 5.161 153 TGGTATTGTGCATATACATGTGTGT 111 25 0 36.00 59.828 18.75 0.00 42.66 5.172 154 TGTGGTATTGTGCATATACATGTGT 109 25 0 36.00 59.828 8.22 0.00 34.94 5.172 155 TGTGTGGTATTGTGCATATACATGT 107 25 0 36.00 59.828 8.22 3.20 34.94 5.172 156 ACATGTGTGGTATTGTGCATATACA 104 25 0 36.00 59.828 18.75 7.54 0.00 5.172 157 GTAGCTTCTTGACTGTGTGTTGTG 147 24 0 45.83 61.177 0.00 0.00 0.00 5.177 158 CATGTGTGTTGTGTATGGATGTAG 127 24 0 41.67 58.810 0.00 0.00 0.00 5.190 159 GTTGTGTATGGATGTAGCTTCTTG 134 24 0 41.67 58.809 0.00 0.00 0.00 5.191 160 CATGTGTGTTGTGTATGGATGTA 127 23 0 39.13 57.795 0.00 0.00 0.00 5.205 161 TACATGTGTGTTGTGTATGGATG 125 23 0 39.13 57.795 9.61 0.00 40.30 5.205 162 TGTGTGTTGTGTATGGATGTAGCT 129 24 0 41.67 61.209 0.00 0.00 0.00 5.209 163 GTTAAACATGTGTGGTATTGTGCAT 99 25 0 36.00 59.776 0.00 0.00 0.00 5.224 164 ATACATGTGTGTTGTGTATGGATGT 124 25 0 36.00 59.769 9.61 0.00 38.35 5.231 165 AACATGTGTGGTATTGTGCATAT 103 23 0 34.78 57.763 0.00 0.00 0.00 5.237 166 CTGTTAAACATGTGTGGTATTGTGC 97 25 0 40.00 60.292 0.00 0.00 0.00 5.292 167 TGTTGTGTATGGATGTAGCTTCTTG 133 25 0 40.00 60.294 0.00 0.00 0.00 5.294 168 ACATGTGTGTTGTGTATGGATGTAG 126 25 0 40.00 60.294 0.00 0.00 34.85 5.294 169 GTGTTGTGTATGGATGTAGCTTCTT 132 25 0 40.00 60.294 0.00 0.00 0.00 5.294 170 GTATGGATGTAGCTTCTTGACTG 139 23 0 43.48 57.686 0.00 0.00 0.00 5.314 171 ATTGTGCATATACATGTGTGTTGTG 115 25 0 36.00 59.605 3.23 0.00 40.30 5.395 172 TTAAACATGTGTGGTATTGTGCAT 100 24 0 33.33 58.600 0.00 0.00 0.00 5.400 173 GTATGGATGTAGCTTCTTGACTGTG 139 25 0 44.00 60.408 0.00 0.00 0.00 5.408 174 GTGTATGGATGTAGCTTCTTGACTG 137 25 0 44.00 60.408 0.00 0.00 0.00 5.408 175 GGTGTGTCTGTTAAACATGTGTGG 90 24 0 45.83 61.422 0.00 0.00 0.00 5.422 176 GGGTGTGTCTGTTAAACATGTGTG 89 24 0 45.83 61.422 0.00 0.00 0.00 5.422 177 GTGGGTGTGTCTGTTAAACATGTG 87 24 0 45.83 61.422 0.00 0.00 0.00 5.422 178 TTGTGTATGGATGTAGCTTCTTG 135 23 0 39.13 57.536 0.00 0.00 0.00 5.464 179 ATGTGTGTTGTGTATGGATGTAG 128 23 0 39.13 57.536 0.00 0.00 0.00 5.464 180 GTTGTGTATGGATGTAGCTTCTT 134 23 0 39.13 57.535 0.00 0.00 0.00 5.465 181 GTGCATATACATGTGTGTTGTGTA 118 24 0 37.50 58.507 3.23 0.00 40.30 5.493 182 GCATATACATGTGTGTTGTGTATGG 120 25 0 40.00 59.494 17.56 6.84 44.43 5.506 183 GTGGTATTGTGCATATACATGTGTG 110 25 0 40.00 59.494 8.22 4.60 34.94 5.506 184 GTGTGGTATTGTGCATATACATGTG 108 25 0 40.00 59.494 9.61 0.00 34.94 5.506 185 TCTTGTGGGTGTGTCTGTTAAACA 83 24 0 41.67 61.514 0.00 0.00 0.00 5.514 186 TGTCTTGTGGGTGTGTCTGTTAAA 81 24 0 41.67 61.514 0.00 0.00 0.00 5.514 187 TAGCTTCTTGACTGTGTGTTGTGT 148 24 0 41.67 61.564 0.00 0.00 0.00 5.564 188 TGTAGCTTCTTGACTGTGTGTTGT 146 24 0 41.67 61.564 0.00 0.00 0.00 5.564 189 ATTGTGCATATACATGTGTGTTGT 115 24 0 33.33 58.422 3.23 0.00 40.30 5.578 190 AAACATGTGTGGTATTGTGCATAT 102 24 0 33.33 58.412 0.00 0.00 0.00 5.588 191 GTGTCTGTTAAACATGTGTGGTAT 93 24 0 37.50 58.375 0.00 0.00 0.00 5.625 192 GTGTGTCTGTTAAACATGTGTGGTA 91 25 0 40.00 60.639 0.00 0.00 0.00 5.639 193 TGGTATTGTGCATATACATGTGTG 111 24 0 37.50 58.313 18.75 4.60 34.94 5.687 194 TGTGGTATTGTGCATATACATGTG 109 24 0 37.50 58.313 9.61 0.00 34.94 5.687 195 TGTGTGGTATTGTGCATATACATG 107 24 0 37.50 58.313 8.22 0.00 35.35 5.687 196 CATGTGTGGTATTGTGCATATACA 105 24 0 37.50 58.313 18.75 7.54 0.00 5.687 197 GGTATTGTGCATATACATGTGTGT 112 24 0 37.50 58.312 18.75 0.00 42.66 5.688 198 GTGGTATTGTGCATATACATGTGT 110 24 0 37.50 58.312 8.22 0.00 34.94 5.688 199 GTGTGGTATTGTGCATATACATGT 108 24 0 37.50 58.312 8.22 3.20 34.94 5.688 200 ACATGTGTGGTATTGTGCATATAC 104 24 0 37.50 58.312 12.13 12.13 0.00 5.688 201 TTGTGCATATACATGTGTGTTGTGT 116 25 0 36.00 60.700 3.23 0.00 40.30 5.700 202 TGGATGTAGCTTCTTGACTGTGTG 142 24 0 45.83 61.738 0.00 0.00 0.00 5.738 203 GGATGTAGCTTCTTGACTGTGTGT 143 24 0 45.83 61.739 0.00 0.00 0.00 5.739 204 ATACATGTGTGTTGTGTATGGATG 124 24 0 37.50 58.249 9.61 0.00 38.35 5.751 205 TACATGTGTGTTGTGTATGGATGTA 125 25 0 36.00 59.243 9.61 0.00 40.30 5.757 206 TATGGATGTAGCTTCTTGACTGTGT 140 25 0 40.00 60.765 0.00 0.00 0.00 5.765 207 TGTATGGATGTAGCTTCTTGACTGT 138 25 0 40.00 60.765 0.00 0.00 0.00 5.765 208 TGTGTATGGATGTAGCTTCTTGACT 136 25 0 40.00 60.765 0.00 0.00 0.00 5.765 209 TGCATATACATGTGTGTTGTGTA 119 23 0 34.78 57.213 3.23 0.00 40.30 5.787 210 TGGGTGTGTCTGTTAAACATGTGT 88 24 0 41.67 61.817 0.00 0.00 0.00 5.817 211 TGTGGGTGTGTCTGTTAAACATGT 86 24 0 41.67 61.817 0.00 0.00 0.00 5.817 212 TGTTAAACATGTGTGGTATTGTGCA 98 25 0 36.00 60.875 0.00 0.00 0.00 5.875 213 TGTCTGTTAAACATGTGTGGTAT 94 23 0 34.78 57.063 0.00 0.00 0.00 5.937 214 GGTATTGTGCATATACATGTGTGTTG 112 26 0 38.46 60.015 18.75 0.00 40.30 6.015 215 GTGCATATACATGTGTGTTGTGTATG 118 26 0 38.46 60.016 13.84 13.84 45.64 6.016 216 TCTGTTAAACATGTGTGGTATTGTG 96 25 0 36.00 58.967 0.00 0.00 0.00 6.033 217 TGTCTGTTAAACATGTGTGGTATTG 94 25 0 36.00 58.967 0.00 0.00 0.00 6.033 218 GTCTGTTAAACATGTGTGGTATTGT 95 25 0 36.00 58.967 0.00 0.00 0.00 6.033 219 GTGTCTGTTAAACATGTGTGGTATT 93 25 0 36.00 58.967 0.00 0.00 0.00 6.033 220 GAACATGTGTGGCATTGTGTATACA 21 25 0 40.00 61.041 7.27 4.43 35.88 6.041 221 GTCTGTTAAACATGTGTGGTATTGTG 95 26 0 38.46 60.070 0.00 0.00 0.00 6.070 222 GTGTCTGTTAAACATGTGTGGTATTG 93 26 0 38.46 60.070 0.00 0.00 0.00 6.070 223 TGCATATACATGTGTGTTGTGTATG 119 25 0 36.00 58.914 13.84 13.84 45.64 6.086 224 GTGCATATACATGTGTGTTGTGTAT 118 25 0 36.00 58.914 4.13 3.79 41.36 6.086 225 GTGTGTTGTGTATGGATGTAGCTTC 130 25 0 44.00 61.090 0.00 0.00 0.00 6.090 226 GGTATTGTGCATATACATGTGTGTT 112 25 0 36.00 58.907 18.75 0.00 40.30 6.093 227 AACATGTGTGGTATTGTGCATATAC 103 25 0 36.00 58.907 12.13 12.13 0.00 6.093 228 GTGTCTTGTGGGTGTGTCTGTTAA 80 24 0 45.83 62.097 0.00 0.00 0.00 6.097 229 TACATGTGTGTTGTGTATGGATGTAG 125 26 0 38.46 60.124 9.61 0.00 40.30 6.124 230 TAAACATGTGTGGTATTGTGCATA 101 24 0 33.33 57.868 0.00 0.00 0.00 6.132 231 CATGTGTGGTATTGTGCATATACATG 105 26 0 38.46 59.850 13.99 9.60 35.35 6.150 232 ATGTGTGGTATTGTGCATATACATGT 106 26 0 34.62 60.180 8.22 3.20 34.94 6.180 233 ACATGTGTGGTATTGTGCATATACAT 104 26 0 34.62 60.180 8.22 0.00 0.00 6.180 234 TGTGTTGTGTATGGATGTAGCTTCT 131 25 0 40.00 61.231 0.00 0.00 0.00 6.231 235 GATGTAGCTTCTTGACTGTGTGTTG 144 25 0 44.00 61.257 0.00 0.00 0.00 6.257 236 TCTGTTAAACATGTGTGGTATTGT 96 24 0 33.33 57.742 0.00 0.00 0.00 6.258 237 TGTCTGTTAAACATGTGTGGTATT 94 24 0 33.33 57.742 0.00 0.00 0.00 6.258 238 ATGTGTGGTATTGTGCATATACATG 106 25 0 36.00 58.730 8.22 0.00 35.35 6.270 239 CATGTGTGGTATTGTGCATATACAT 105 25 0 36.00 58.730 8.22 0.00 0.00 6.270 240 AACATGTGTGGCATTGTGTATACAC 22 25 0 40.00 61.271 26.66 26.66 41.05 6.271 241 TGCATATACATGTGTGTTGTGTAT 119 24 0 33.33 57.692 3.79 3.79 41.36 6.308 242 CTGTTAAACATGTGTGGTATTGTG 97 24 0 37.50 57.686 0.00 0.00 0.00 6.314 243 AACATGTGTGGTATTGTGCATATA 103 24 0 33.33 57.678 0.00 0.00 0.00 6.322 244 TGTGCATATACATGTGTGTTGTGTAT 117 26 0 34.62 60.348 4.13 3.79 41.36 6.348 245 TGGTATTGTGCATATACATGTGTGTT 111 26 0 34.62 60.349 18.75 0.00 40.30 6.349 246 AACATGTGTGGTATTGTGCATATACA 103 26 0 34.62 60.349 18.75 7.54 0.00 6.349 247 CATGTGTGTTGTGTATGGATGTAGC 127 25 0 44.00 61.374 0.00 0.00 0.00 6.374 248 GTTAAACATGTGTGGTATTGTGCATA 99 26 0 34.62 59.626 0.00 0.00 0.00 6.374 249 ATACATGTGTGTTGTGTATGGATGTA 124 26 0 34.62 59.616 9.61 0.00 38.35 6.384 250 TATACATGTGTGTTGTGTATGGATGT 123 26 0 34.62 59.616 7.92 0.00 39.64 6.384 251 TGTCTGTTAAACATGTGTGGTATTGT 94 26 0 34.62 60.405 0.00 0.00 0.00 6.405 252 TGTGTCTGTTAAACATGTGTGGTATT 92 26 0 34.62 60.405 0.00 0.00 0.00 6.405 253 TTAAACATGTGTGGTATTGTGCATA 100 25 0 32.00 58.487 0.00 0.00 0.00 6.513 254 ATGTGTGGTATTGTGCATATACAT 106 24 0 33.33 57.486 8.22 0.00 0.00 6.514 255 TTGTGCATATACATGTGTGTTGTGTA 116 26 0 34.62 60.515 3.23 0.00 40.30 6.515 256 ATGTGTGTTGTGTATGGATGTAGCT 128 25 0 40.00 61.523 0.00 0.00 0.00 6.523 257 TATTGTGCATATACATGTGTGTTGTG 114 26 0 34.62 59.461 3.23 0.00 40.30 6.539 258 GTATTGTGCATATACATGTGTGTTGT 113 26 0 34.62 59.461 14.51 0.00 40.30 6.539 259 AAACATGTGTGGTATTGTGCATATAC 102 26 0 34.62 59.457 12.13 12.13 0.00 6.543 260 GTCTGTTAAACATGTGTGGTATTG 95 24 0 37.50 57.446 0.00 0.00 0.00 6.554 261 GCATATACATGTGTGTTGTGTATG 120 24 0 37.50 57.398 13.84 13.84 45.64 6.602 262 TGTGTTGTGTATAAATGTTGTGTCT 50 25 0 32.00 58.377 0.00 0.00 0.00 6.623 263 CTTGTGGGTGTGTCTGTTAAACATG 84 25 0 44.00 61.663 0.00 0.00 0.00 6.663 264 GCATATACATGTGTGTTGTGTATGGA 120 26 0 38.46 60.680 17.56 0.00 44.43 6.680 265 TATTGTGCATATACATGTGTGTTGT 114 25 0 32.00 58.316 3.23 0.00 40.30 6.684 266 AAACATGTGTGGTATTGTGCATATA 102 25 0 32.00 58.306 0.00 0.00 0.00 6.694 267 TAAACATGTGTGGTATTGTGCATAT 101 25 0 32.00 58.306 0.00 0.00 0.00 6.694 268 TGTGTGTTGTGTATGGATGTAGCTT 129 25 0 40.00 61.694 0.00 0.00 0.00 6.694 269 TCTTGTGGGTGTGTCTGTTAAACAT 83 25 0 40.00 61.817 0.00 0.00 0.00 6.817 270 AGCTTCTTGACTGTGTGTTGTGTG 149 24 0 45.83 62.850 0.00 0.00 0.00 6.850 271 TATACATGTGTGTTGTGTATGGATG 123 25 0 36.00 58.150 7.92 0.00 39.64 6.850 272 CATATACATGTGTGTTGTGTATGGA 121 25 0 36.00 58.150 11.52 0.00 42.41 6.850 273 ATGTAGCTTCTTGACTGTGTGTTGT 145 25 0 40.00 61.863 0.00 0.00 0.00 6.863 274 TGCATATACATGTGTGTTGTGTATGG 119 26 0 38.46 60.902 17.56 6.84 44.43 6.902 275 TGTGGTATTGTGCATATACATGTGTG 109 26 0 38.46 60.902 8.22 4.60 34.94 6.902 276 TGTGTGGTATTGTGCATATACATGTG 107 26 0 38.46 60.902 9.61 0.00 34.94 6.902 277 GTGGTATTGTGCATATACATGTGTGT 110 26 0 38.46 60.902 9.41 0.00 42.66 6.902 278 GTGTGGTATTGTGCATATACATGTGT 108 26 0 38.46 60.902 8.22 0.00 34.94 6.902 279 TGTGTTGTGTATAAATGTTGTGTC 50 24 0 33.33 57.075 0.00 0.00 0.00 6.925 280 GTGTGTCTGTTAAACATGTGTGGTAT 91 26 0 38.46 60.957 0.00 0.00 0.00 6.957 281 GTATTGTGCATATACATGTGTGTTG 113 25 0 36.00 58.019 14.51 0.00 40.30 6.981 282 ATTGTGCATATACATGTGTGTTGTGT 115 26 0 34.62 61.018 3.23 0.00 40.30 7.018 283 ATGGATGTAGCTTCTTGACTGTGTG 141 25 0 44.00 62.029 0.00 0.00 0.00 7.029 284 GTTAAACATGTGTGGTATTGTGCATAT 99 27 0 33.33 59.967 0.00 0.00 0.00 7.033 285 ATATACATGTGTGTTGTGTATGGATGT 122 27 0 33.33 59.963 7.92 0.00 39.64 7.037 286 TGTGTTGTGTATAAATGTTGTGTCTT 50 26 0 30.77 58.950 0.00 0.00 0.00 7.050 287 TTAAACATGTGTGGTATTGTGCATAT 100 26 0 30.77 58.886 0.00 0.00 0.00 7.114 288 GTTGTGTATGGATGTAGCTTCTTGAC 134 26 0 42.31 61.115 0.00 0.00 0.00 7.115 289 TGTTAAACATGTGTGGTATTGTGCAT 98 26 0 34.62 61.187 0.00 0.00 0.00 7.187 290 GTGTTGTGTATAAATGTTGTGTCTTCA 51 27 0 33.33 59.809 0.00 0.00 0.00 7.191 291 TGTGTTGTGTATAAATGTTGTGTCTTC 50 27 0 33.33 59.809 0.00 0.00 0.00 7.191 292 GGATGTAGCTTCTTGACTGTGTGTT 143 25 0 44.00 62.198 0.00 0.00 0.00 7.198 293 ATGTGTTGTGTATAAATGTTGTGTCT 49 26 0 30.77 58.778 0.00 0.00 0.00 7.222 294 TTGTGTATGGATGTAGCTTCTTGACT 135 26 0 38.46 61.252 0.00 0.00 0.00 7.252 295 TGTTGTGTATAAATGTTGTGTCTTCA 52 26 0 30.77 58.726 0.00 0.00 0.00 7.274 296 TTGTGGGTGTGTCTGTTAAACATGT 85 25 0 40.00 62.278 0.00 0.00 0.00 7.278 297 GTGTTGTGTATGGATGTAGCTTCTTG 132 26 0 42.31 61.334 0.00 0.00 0.00 7.334 298 CATATACATGTGTGTTGTGTATGGATG 121 27 0 37.04 59.649 11.52 3.24 42.41 7.351 299 ATATACATGTGTGTTGTGTATGGATG 122 26 0 34.62 58.558 7.92 0.00 39.64 7.442 300 CATATACATGTGTGTTGTGTATGGAT 121 26 0 34.62 58.558 11.52 0.00 42.41 7.442 301 ATACATGTGTGTTGTGTATGGATGTAG 124 27 0 37.04 60.450 9.61 0.00 38.35 7.450 302 TCTGTTAAACATGTGTGGTATTGTGC 96 26 0 38.46 61.452 0.00 0.00 0.00 7.452 303 ATGTGTTGTGTATAAATGTTGTGTC 49 25 0 32.00 57.534 0.00 0.00 0.00 7.466 304 TGTTGTGTATGGATGTAGCTTCTTGA 133 26 0 38.46 61.474 0.00 0.00 0.00 7.474 305 GTATTGTGCATATACATGTGTGTTGTG 113 27 0 37.04 60.497 14.51 0.00 40.30 7.497 306 GTGTTGTGTATAAATGTTGTGTCTT 51 25 0 32.00 57.477 0.00 0.00 0.00 7.523 307 TATACATGTGTGTTGTGTATGGATGTA 123 27 0 33.33 59.474 7.92 0.00 39.64 7.526 308 GTCTTGTGGGTGTGTCTGTTAAACA 82 25 0 44.00 62.541 0.00 0.00 0.00 7.541 309 TGTCTTGTGGGTGTGTCTGTTAAAC 81 25 0 44.00 62.541 0.00 0.00 0.00 7.541 310 GTGTCTTGTGGGTGTGTCTGTTAAA 80 25 0 44.00 62.541 0.00 0.00 0.00 7.541 311 GTGTTGTGTATAAATGTTGTGTCTTC 51 26 0 34.62 58.430 0.00 0.00 0.00 7.570 312 TAGCTTCTTGACTGTGTGTTGTGTG 148 25 0 44.00 62.579 0.00 0.00 0.00 7.579 313 TGTAGCTTCTTGACTGTGTGTTGTG 146 25 0 44.00 62.579 0.00 0.00 0.00 7.579 314 GTAGCTTCTTGACTGTGTGTTGTGT 147 25 0 44.00 62.581 0.00 0.00 0.00 7.581 315 ATATACATGTGTGTTGTGTATGGAT 122 25 0 32.00 57.351 7.92 0.00 39.64 7.649 316 AACATGTGTGGTATTGTGCATATACAT 103 27 0 33.33 60.670 8.22 0.00 0.00 7.670 317 CTGTTAAACATGTGTGGTATTGTGCA 97 26 0 38.46 61.672 0.00 0.00 0.00 7.672 318 TAAACATGTGTGGTATTGTGCATATAC 101 27 0 33.33 59.323 12.13 12.13 0.00 7.677 319 ATGTGTTGTGTATAAATGTTGTGTCTT 49 27 0 29.63 59.317 0.00 0.00 0.00 7.683 320 TGTGTTGTGTATGGATGTAGCTTCTT 131 26 0 38.46 61.698 0.00 0.00 0.00 7.698 321 GTTGTGTATAAATGTTGTGTCTTCA 53 25 0 32.00 57.246 0.00 0.00 0.00 7.754 322 TGTTGTGTATAAATGTTGTGTCTTC 52 25 0 32.00 57.246 0.00 0.00 0.00 7.754 323 TAAACATGTGTGGTATTGTGCATATA 101 26 0 30.77 58.208 0.00 0.00 0.00 7.792 324 TATGGATGTAGCTTCTTGACTGTGTG 140 26 0 42.31 61.796 0.00 0.00 0.00 7.796 325 TGTATGGATGTAGCTTCTTGACTGTG 138 26 0 42.31 61.796 0.00 0.00 0.00 7.796 326 TGTGTATGGATGTAGCTTCTTGACTG 136 26 0 42.31 61.796 0.00 0.00 0.00 7.796 327 GTATGGATGTAGCTTCTTGACTGTGT 139 26 0 42.31 61.797 0.00 0.00 0.00 7.797 328 GTGTATGGATGTAGCTTCTTGACTGT 137 26 0 42.31 61.797 0.00 0.00 0.00 7.797 329 TGGGTGTGTCTGTTAAACATGTGTG 88 25 0 44.00 62.827 0.00 0.00 0.00 7.827 330 TGTGGGTGTGTCTGTTAAACATGTG 86 25 0 44.00 62.827 0.00 0.00 0.00 7.827 331 TTGTGCATATACATGTGTGTTGTGTAT 116 27 0 33.33 60.828 4.13 3.79 41.36 7.828 332 ATTGTGCATATACATGTGTGTTGTGTA 115 27 0 33.33 60.828 3.23 0.00 40.30 7.828 333 TATTGTGCATATACATGTGTGTTGTGT 114 27 0 33.33 60.828 4.13 0.00 40.30 7.828 334 GGTGTGTCTGTTAAACATGTGTGGT 90 25 0 44.00 62.829 0.00 0.00 0.00 7.829 335 GTGGGTGTGTCTGTTAAACATGTGT 87 25 0 44.00 62.829 0.00 0.00 0.00 7.829 336 AAACATGTGTGGTATTGTGCATATACA 102 27 0 33.33 60.831 18.75 7.54 0.00 7.831 337 ATGTGTGTTGTGTATGGATGTAGCTT 128 26 0 38.46 61.979 0.00 0.00 0.00 7.979 338 GCATATACATGTGTGTTGTGTATGGAT 120 27 0 37.04 60.986 17.56 0.00 44.43 7.986 339 TGTTAAACATGTGTGGTATTGTGCATA 98 27 0 33.33 60.991 0.00 0.00 0.00 7.991 340 GAACATGTGTGGCATTGTGTATACAC 21 26 0 42.31 62.039 26.66 26.66 41.05 8.039 341 ATGTGTGGTATTGTGCATATACATGTG 106 27 0 37.04 61.200 9.61 0.00 34.94 8.200 342 CATGTGTGGTATTGTGCATATACATGT 105 27 0 37.04 61.200 15.63 3.20 34.94 8.200 343 ACATGTGTGGTATTGTGCATATACATG 104 27 0 37.04 61.200 17.52 16.48 36.76 8.200 344 TTAAACATGTGTGGTATTGTGCATATA 100 27 0 29.63 58.771 0.00 0.00 0.00 8.229 345 GTGTGTTGTGTATGGATGTAGCTTCT 130 26 0 42.31 62.236 0.00 0.00 0.00 8.236 346 TATGTGTTGTGTATAAATGTTGTGTCT 48 27 0 29.63 58.667 0.00 0.00 0.00 8.333 347 TGTGCATATACATGTGTGTTGTGTATG 117 27 0 37.04 61.352 13.84 13.84 45.64 8.352 348 TGGTATTGTGCATATACATGTGTGTTG 111 27 0 37.04 61.358 18.75 0.00 40.30 8.358 349 GGTATTGTGCATATACATGTGTGTTGT 112 27 0 37.04 61.358 18.75 0.00 40.30 8.358 350 GTGGTATTGTGCATATACATGTGTGTT 110 27 0 37.04 61.358 9.41 0.00 40.30 8.358 351 TGTCTGTTAAACATGTGTGGTATTGTG 94 27 0 37.04 61.410 0.00 0.00 0.00 8.410 352 TGTGTCTGTTAAACATGTGTGGTATTG 92 27 0 37.04 61.410 0.00 0.00 0.00 8.410 353 GTGTCTGTTAAACATGTGTGGTATTGT 93 27 0 37.04 61.410 0.00 0.00 0.00 8.410 354 GTGTGTCTGTTAAACATGTGTGGTATT 91 27 0 37.04 61.410 0.00 0.00 0.00 8.410 355 TGTGTGTTGTGTATGGATGTAGCTTC 129 26 0 42.31 62.452 0.00 0.00 0.00 8.452 356 CATGTGTGTTGTGTATGGATGTAGCT 127 26 0 42.31 62.512 0.00 0.00 0.00 8.512 357 TATGTGTTGTGTATAAATGTTGTGTC 48 26 0 30.77 57.465 0.00 0.00 0.00 8.535 358 GGTGTGTCTGTTAAACATGTGTGGTA 90 26 0 42.31 62.567 0.00 0.00 0.00 8.567 359 GATGTAGCTTCTTGACTGTGTGTTGT 144 26 0 42.31 62.610 0.00 0.00 0.00 8.610 360 ACATGTGTGTTGTGTATGGATGTAGC 126 26 0 42.31 62.728 0.00 0.00 34.85 8.728 361 TCTTGTGGGTGTGTCTGTTAAACATG 83 26 0 42.31 62.792 0.00 0.00 0.00 8.792 362 GTCTTGTGGGTGTGTCTGTTAAACAT 82 26 0 42.31 62.793 0.00 0.00 0.00 8.793 363 ATGTAGCTTCTTGACTGTGTGTTGTG 145 26 0 42.31 62.828 0.00 0.00 0.00 8.828 364 GTGCATATACATGTGTGTTGTGTATGG 118 27 0 40.74 61.873 17.56 6.84 44.43 8.873 365 GTGTGGTATTGTGCATATACATGTGTG 108 27 0 40.74 61.873 8.22 4.60 34.94 8.873 366 CATATGTGTTGTGTATAAATGTTGTGT 46 27 0 29.63 58.095 0.00 0.00 0.00 8.905 367 ACATATGTGTTGTGTATAAATGTTGTG 45 27 0 29.63 58.095 8.19 0.00 34.85 8.905 368 CACATATGTGTTGTGTATAAATGTTGT 44 27 0 29.63 58.095 25.59 0.00 41.45 8.905 369 CTGTTAAACATGTGTGGTATTGTGCAT 97 27 0 37.04 61.943 0.00 0.00 0.00 8.943 370 TGCATATACATGTGTGTTGTGTATGGA 119 27 0 37.04 62.020 17.56 14.16 44.43 9.020 371 ATATGTGTTGTGTATAAATGTTGTGTC 47 27 0 29.63 57.879 0.00 0.00 0.00 9.121 372 TTGTGTATGGATGTAGCTTCTTGACTG 135 27 0 40.74 62.221 0.00 0.00 0.00 9.221 373 GTTGTGTATGGATGTAGCTTCTTGACT 134 27 0 40.74 62.222 0.00 0.00 0.00 9.222 374 TGTGGTATTGTGCATATACATGTGTGT 109 27 0 37.04 62.236 9.41 0.00 42.66 9.236 375 TGTGTGGTATTGTGCATATACATGTGT 107 27 0 37.04 62.236 8.22 0.00 34.94 9.236 376 GTCTGTTAAACATGTGTGGTATTGTGC 95 27 0 40.74 62.394 0.00 0.00 0.00 9.394 377 TGTTGTGTATGGATGTAGCTTCTTGAC 133 27 0 40.74 62.430 0.00 0.00 0.00 9.430 378 GTGTTGTGTATGGATGTAGCTTCTTGA 132 27 0 40.74 62.430 0.00 0.00 0.00 9.430 379 TACATGTGTGTTGTGTATGGATGTAGC 125 27 0 40.74 62.480 9.61 0.00 40.30 9.480 380 TGTGTTGTGTATGGATGTAGCTTCTTG 131 27 0 40.74 62.643 0.00 0.00 0.00 9.643 381 GTGTGTTGTGTATGGATGTAGCTTCTT 130 27 0 40.74 62.644 0.00 0.00 0.00 9.644 382 ATGTGTGTTGTGTATGGATGTAGCTTC 128 27 0 40.74 62.698 0.00 0.00 0.00 9.698 383 GTATGGATGTAGCTTCTTGACTGTGTG 139 27 0 44.44 62.728 0.00 0.00 0.00 9.728 384 GTGTATGGATGTAGCTTCTTGACTGTG 137 27 0 44.44 62.728 0.00 0.00 0.00 9.728 385 TCTGTTAAACATGTGTGGTATTGTGCA 96 27 0 37.04 62.765 0.00 0.00 0.00 9.765 386 GGTGTGTCTGTTAAACATGTGTGGTAT 90 27 0 40.74 62.809 0.00 0.00 0.00 9.809 387 CATGTGTGTTGTGTATGGATGTAGCTT 127 27 0 40.74 62.910 0.00 0.00 0.00 9.910 primer3-2.3.6/test/primer1_th_list_last/SP01-20.txt.rev0000664000077300007730000000104112231156243021415 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 CCCCAACGGCCCCAGATT 118 18 0 66.67 62.999 0.00 0.00 0.00 4.999 1 CCCAACGGCCCCAGATTT 117 18 0 61.11 60.890 0.00 0.00 0.00 2.890 2 CCAACGGCCCCAGATTTC 116 18 0 61.11 59.299 0.00 0.00 0.00 2.701 3 CCCAACGGCCCCAGATTTC 117 19 0 63.16 61.971 0.00 0.00 0.00 2.971 primer3-2.3.6/test/primer1_th_list_last/long_seq.for0000664000077300007730000023220012231156243021501 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 tgctagctaggcgatgctag 411 20 0 55.00 60.028 23.16 23.16 38.59 0.028 1 actgatacgcgatgctagct 476 20 0 50.00 59.957 17.69 1.35 0.00 0.043 2 gatcgatgctagctaggcga 405 20 0 55.00 60.100 16.30 16.30 0.00 0.100 3 tcgatcgatgctagctaggc 403 20 0 55.00 60.100 18.63 8.45 0.00 0.100 4 tagctgatcgatcgtagcgg 565 20 0 55.00 60.101 25.02 17.36 0.00 0.101 5 gctgactgatcgatcgatgc 113 20 0 55.00 59.826 24.08 17.09 35.21 0.174 6 tatcatctctgcgcgatcga 361 20 0 50.00 59.747 22.07 1.72 38.48 0.253 7 agctaggcgatgctagctag 415 20 0 55.00 59.742 17.46 17.46 41.54 0.258 8 ctagctaggcgatgctagct 413 20 0 55.00 59.742 18.68 17.35 43.53 0.258 9 ggcgatctagctagctgact 583 20 0 55.00 59.671 17.44 7.44 37.58 0.329 10 tcgatgctagctaggcgatg 407 20 0 55.00 60.382 14.03 0.00 0.00 0.382 11 gctgatcgatcgatgctagc 398 20 0 55.00 59.618 25.97 24.79 35.21 0.382 12 gctagctgatcgatcgatgc 394 20 0 55.00 59.618 24.08 21.09 35.21 0.382 13 atcatctctgcgcgatcgat 362 20 0 50.00 60.382 22.07 5.02 38.48 0.382 14 gactgatacgcgatgctagc 475 20 0 55.00 59.551 8.61 8.61 0.00 0.449 15 atcgatgctagctaggcgat 406 20 0 50.00 59.452 18.43 18.43 0.00 0.548 16 gctagctgactgatacgcga 468 20 0 55.00 60.589 16.29 0.00 0.00 0.589 17 agctagctgactgatacgcg 467 20 0 55.00 60.590 17.99 3.89 0.00 0.590 18 atgctagctaggcgatgcta 410 20 0 50.00 59.375 10.59 8.91 0.00 0.625 19 ctatcatctctgcgcgatcg 360 20 0 55.00 59.347 12.19 12.19 39.07 0.653 20 gatgctagctaggcgatgct 409 20 0 55.00 60.668 7.01 7.53 0.00 0.668 21 gctactatcatctctgcgcg 356 20 0 55.00 59.273 0.00 0.00 0.00 0.727 22 cgtagcggcgatctagctag 577 20 0 60.00 60.791 15.64 15.64 37.58 0.791 23 cggcgatctagctagctgac 582 20 0 60.00 61.003 14.84 7.25 38.70 1.003 24 gctagctgatcgatcgtagc 563 20 0 55.00 58.995 23.70 23.70 0.00 1.005 25 gatcgatcgatgtgcggcta 81 20 0 55.00 61.006 19.16 0.00 41.65 1.006 26 atcgatcgatgtgcggctag 82 20 0 55.00 61.008 29.65 0.00 41.65 1.008 27 gctgactgatacgcgatgc 472 19 0 57.89 60.025 0.00 0.00 0.00 1.025 28 agctagctgatcatcgatgct 190 21 0 47.62 60.035 17.99 11.09 0.00 1.035 29 gctagctagctgactgatcga 105 21 0 52.38 60.037 34.38 0.00 46.11 1.037 30 tcatctctgcgcgatcgat 363 19 0 52.63 59.946 22.07 0.12 38.48 1.054 31 atcatctctgcgcgatcga 362 19 0 52.63 59.946 22.07 1.72 38.48 1.054 32 atcgatcgatgtgcggcta 82 19 0 52.63 59.945 29.65 0.00 41.65 1.055 33 gtagcggcgatctagctagc 578 20 0 60.00 61.071 16.97 7.15 39.86 1.071 34 gctagctgactgatcgatcg 109 20 0 55.00 58.924 16.84 13.89 0.00 1.076 35 gctgatcgatcgatgtgcg 78 19 0 57.89 60.097 29.87 18.15 42.69 1.097 36 tatcatctctgcgcgatcgat 361 21 0 47.62 60.172 22.07 11.47 38.48 1.172 37 gctagctagctgatcgatcga 390 21 0 52.38 60.172 34.38 22.52 46.11 1.172 38 gctagctagctgatcgatcga 70 21 0 52.38 60.172 34.38 22.52 46.11 1.172 39 catctctgcgcgatcgatg 364 19 0 57.89 59.810 13.74 13.74 38.48 1.190 40 tcgtagcggcgatctagcta 576 20 0 55.00 61.231 11.55 9.27 36.40 1.231 41 actgatacgcgatgctagcta 476 21 0 47.62 59.765 17.69 3.08 0.00 1.235 42 actgatcgatcgatgctagct 117 21 0 47.62 59.763 23.29 11.70 35.21 1.237 43 agctagctgatcgatcgatgt 73 21 0 47.62 59.763 17.99 2.62 35.21 1.237 44 tagcggcgatctagctagct 579 20 0 55.00 61.243 23.74 23.74 46.60 1.243 45 cgtagcggcgatctagcta 577 19 0 57.89 59.729 11.55 9.27 37.58 1.271 46 ctagctgatcgatcgtagcg 564 20 0 55.00 58.727 25.02 15.05 0.00 1.273 47 tagcggcgatctagctagc 579 19 0 57.89 59.725 16.97 9.14 39.86 1.275 48 catcgatcgatgcatgcatg 442 20 0 50.00 58.722 37.80 23.31 44.93 1.278 49 tcatctctgcgcgatcgatg 363 20 0 55.00 61.279 18.01 18.01 38.48 1.279 50 gctagctagctgatcgatcg 559 20 0 55.00 58.714 34.38 11.90 46.11 1.286 51 gctagctagctgatcgatcg 390 20 0 55.00 58.714 34.38 11.90 46.11 1.286 52 gctagctagctgatcgatcg 70 20 0 55.00 58.714 34.38 11.90 46.11 1.286 53 agcatcggattagctagctga 3 21 0 47.62 59.689 28.29 20.88 0.00 1.311 54 agctgatcgatcgtagcgg 566 19 0 57.89 60.315 25.02 17.36 0.00 1.315 55 cggcgatctagctagctga 582 19 0 57.89 59.650 21.57 16.66 38.70 1.350 56 ctagctgatcgatcgatgtgc 75 21 0 52.38 59.643 31.83 30.04 35.21 1.357 57 gctagctgatcgatcgatgtg 74 21 0 52.38 59.643 12.06 6.93 35.21 1.357 58 gctagctaggcgatgctagc 412 20 0 60.00 61.357 30.41 30.41 46.19 1.357 59 tagctagctgactgatacgcg 466 21 0 52.38 60.373 28.29 3.89 0.00 1.373 60 gctagctgactgatcgatcga 109 21 0 52.38 60.374 22.52 22.52 0.00 1.374 61 agctagctgactgatcgatcg 108 21 0 52.38 60.374 17.99 13.89 0.00 1.374 62 cgatcgatgctagctaggcg 404 20 0 60.00 61.409 15.59 9.14 0.00 1.409 63 gctagctagctgactgatcg 105 20 0 55.00 58.563 34.38 1.84 46.11 1.437 64 atgctagctaggcgatgct 410 19 0 52.63 59.561 10.59 7.53 0.00 1.439 65 agctagctgatcgatcgtagc 562 21 0 52.38 60.441 26.87 26.87 0.00 1.441 66 gctagctagctgatcgatcgt 559 21 0 52.38 60.441 34.38 2.65 46.11 1.441 67 tagctaggcgatgctagctag 414 21 0 52.38 59.559 18.42 17.46 42.44 1.441 68 ctagctaggcgatgctagcta 413 21 0 52.38 59.559 18.69 17.64 42.44 1.441 69 tagctgatcgatcgatgtgc 76 20 0 50.00 58.558 31.83 30.04 35.21 1.442 70 gatgctagctaggcgatgcta 409 21 0 52.38 60.444 9.82 8.91 0.00 1.444 71 atgctagctaggcgatgctag 410 21 0 52.38 60.444 23.16 23.16 38.59 1.444 72 gctagctgatcatcgatgct 191 20 0 50.00 58.539 16.29 12.14 0.00 1.461 73 agctagctgatcatcgatgc 190 20 0 50.00 58.539 21.42 9.22 0.00 1.461 74 gctgactgatacgcgatgct 472 20 0 55.00 61.494 2.33 0.00 0.00 1.494 75 agctgactgatacgcgatgc 471 20 0 55.00 61.494 3.47 0.00 0.00 1.494 76 ggcgatctagctagctgacta 583 21 0 52.38 59.491 17.44 5.40 37.58 1.509 77 gatcgatgctagctaggcgat 405 21 0 52.38 60.510 21.61 21.61 0.00 1.510 78 atcgatcgatgctagctaggc 402 21 0 52.38 60.510 29.65 8.45 33.56 1.510 79 ctgatcgatcgatgtgcgg 79 19 0 57.89 59.447 15.54 5.83 41.65 1.553 80 agctgatcgatcgatgtgcg 77 20 0 55.00 61.556 31.92 20.26 42.69 1.556 81 cgatcatcgatgctagctagc 548 21 0 52.38 59.444 34.89 34.89 46.99 1.556 82 tagctaggcgatgctagcta 414 20 0 50.00 58.433 19.37 17.81 42.44 1.567 83 agctagctactgatcgatgct 303 21 0 47.62 59.415 17.56 11.51 0.00 1.585 84 tcgatcgatgtgcggctag 83 19 0 57.89 60.606 18.63 0.00 41.65 1.606 85 gactgatcgatcgatgctagc 116 21 0 52.38 59.378 15.86 8.45 35.21 1.622 86 agctagctgactgatcgatca 260 21 0 47.62 59.347 26.99 26.99 35.44 1.653 87 ctgactgatacgcgatgctag 473 21 0 52.38 59.312 1.70 0.00 0.00 1.688 88 ctagctgactgatacgcgatg 469 21 0 52.38 59.312 0.00 0.00 0.00 1.688 89 gctactatcatctctgcgcga 356 21 0 52.38 60.707 2.71 2.71 0.00 1.707 90 agctactatcatctctgcgcg 355 21 0 52.38 60.709 0.00 0.00 0.00 1.709 91 actatcatctctgcgcgatc 359 20 0 50.00 58.270 4.99 0.00 0.00 1.730 92 actgatcgatcgatgctagc 117 20 0 50.00 58.270 23.29 13.61 35.21 1.730 93 gctagctgatcgatcgatgt 74 20 0 50.00 58.270 14.29 2.62 35.21 1.730 94 ctatcatctctgcgcgatcga 360 21 0 52.38 60.771 22.07 1.72 38.48 1.771 95 atcgatgctagctaggcgatg 406 21 0 52.38 60.779 21.16 4.37 0.00 1.779 96 tgactgatacgcgatgctag 474 20 0 50.00 58.207 1.70 0.00 0.00 1.793 97 ctgactgatacgcgatgcta 473 20 0 50.00 58.207 2.33 0.00 0.00 1.793 98 tagctgactgatacgcgatg 470 20 0 50.00 58.207 0.00 0.00 0.00 1.793 99 ctgactgatcgatcgatgct 114 20 0 50.00 58.197 26.44 12.40 35.21 1.803 100 agctgactgatcgatcgatg 112 20 0 50.00 58.197 23.05 13.21 35.21 1.803 101 tcggattagctagctgatgc 7 20 0 50.00 58.176 17.46 17.46 40.05 1.824 102 gcatcggattagctagctga 4 20 0 50.00 58.176 28.29 20.88 0.00 1.824 103 agcatcggattagctagctg 3 20 0 50.00 58.171 28.29 10.80 0.00 1.829 104 gatgctagctaggcgatgc 409 19 0 57.89 59.141 4.18 0.00 0.00 1.859 105 ctgatacgcgatgctagctag 477 21 0 52.38 59.113 17.46 17.46 0.00 1.887 106 gctagctgactgatacgcg 468 19 0 57.89 59.086 8.21 3.89 0.00 1.914 107 ctctgcgcgatcgatgctag 367 20 0 60.00 61.946 21.94 18.16 38.48 1.946 108 tctgcgcgatcgatgctag 368 19 0 57.89 60.966 21.94 18.16 38.48 1.966 109 ctctgcgcgatcgatgcta 367 19 0 57.89 60.966 26.61 17.10 38.48 1.966 110 cgatgctagctaggcgatgc 408 20 0 60.00 61.968 11.09 0.00 0.00 1.968 111 gactgatacgcgatgctagct 475 21 0 52.38 60.975 17.69 1.35 0.00 1.975 112 gctagctgactgatacgcgat 468 21 0 52.38 60.975 8.21 0.00 0.00 1.975 113 tgatacgcgatgctagctag 478 20 0 50.00 57.994 17.46 17.46 0.00 2.006 114 ctgatacgcgatgctagcta 477 20 0 50.00 57.994 17.69 3.08 0.00 2.006 115 cgcgatcgatgctagctagc 372 20 0 60.00 62.011 34.89 34.89 43.67 2.011 116 gcgcgatcgatgctagctag 371 20 0 60.00 62.011 21.66 17.46 38.48 2.011 117 ctgatcgatcgatgctagct 399 20 0 50.00 57.983 19.70 2.01 35.21 2.017 118 agctgatcgatcgatgctag 397 20 0 50.00 57.983 27.33 18.05 34.69 2.017 119 ctagctgatcgatcgatgct 395 20 0 50.00 57.983 33.87 33.38 38.16 2.017 120 agctagctgatcgatcgatg 393 20 0 50.00 57.983 21.99 11.03 35.21 2.017 121 ctgatcgatcgatgctagct 118 20 0 50.00 57.983 19.70 2.01 35.21 2.017 122 agctagctgatcgatcgatg 73 20 0 50.00 57.983 21.99 11.03 35.21 2.017 123 catcggattagctagctgatgc 5 22 0 50.00 59.982 24.41 24.41 40.05 2.018 124 gcatcggattagctagctgatg 4 22 0 50.00 59.982 27.81 27.81 33.28 2.018 125 tcgatgctagctaggcgat 407 19 0 52.63 58.964 14.03 3.01 0.00 2.036 126 atcgatgctagctaggcga 406 19 0 52.63 58.964 16.30 16.30 0.00 2.036 127 actatcatctctgcgcgatcg 359 21 0 52.38 61.037 12.19 12.19 39.07 2.037 128 gcgcgatcgatgctagcta 371 19 0 57.89 61.037 21.66 3.08 38.48 2.037 129 gctgatcgatcgatgctagct 398 21 0 52.38 61.044 27.88 12.70 35.21 2.044 130 agctgatcgatcgatgctagc 397 21 0 52.38 61.044 27.33 27.90 34.69 2.044 131 gctagctgatcgatcgatgct 394 21 0 52.38 61.044 33.87 33.38 38.16 2.044 132 agctagctgatcgatcgatgc 393 21 0 52.38 61.044 24.08 21.09 35.21 2.044 133 cgcgatcgatgctagctag 372 19 0 57.89 58.947 22.07 17.46 38.48 2.053 134 tcgtagcggcgatctagc 576 18 0 61.11 59.936 4.70 0.00 36.40 2.064 135 cgtagcggcgatctagct 577 18 0 61.11 59.935 11.03 11.03 37.58 2.065 136 gcggcgatctagctagct 581 18 0 61.11 59.933 23.74 23.74 38.05 2.067 137 agcggcgatctagctagc 580 18 0 61.11 59.933 16.97 7.15 39.86 2.067 138 ctagctgactgatacgcgat 469 20 0 50.00 57.918 1.43 0.00 0.00 2.082 139 ctagctgatcgatcgtagcgg 564 21 0 57.14 61.096 20.31 16.15 0.00 2.096 140 agctactatcatctctgcgc 355 20 0 50.00 57.898 0.00 0.00 0.00 2.102 141 gctagctactgatcgatgct 304 20 0 50.00 57.898 11.51 11.51 0.00 2.102 142 agctagctactgatcgatgc 303 20 0 50.00 57.898 17.56 1.76 0.00 2.102 143 agcatcggattagctagctgat 3 22 0 45.45 60.108 17.84 15.13 0.00 2.108 144 tgctagctaggcgatgcta 411 19 0 52.63 58.881 17.69 8.91 0.00 2.119 145 aagcatcggattagctagctg 2 21 0 47.62 58.879 28.29 10.80 0.00 2.121 146 tctgcgcgatcgatgcta 368 18 0 55.56 59.857 26.61 16.13 38.48 2.143 147 cgatgctagctaggcgatg 408 19 0 57.89 58.856 11.09 0.00 0.00 2.144 148 agctagctgatcatcgatgcta 190 22 0 45.45 59.845 17.99 13.09 0.00 2.155 149 tagctagctgatcatcgatgct 189 22 0 45.45 59.845 16.29 15.30 0.00 2.155 150 atcgatcgatgtgcggct 82 18 0 55.56 60.166 29.65 4.36 41.65 2.166 151 gctagctgactgatcgatca 261 20 0 50.00 57.829 26.99 26.99 35.44 2.171 152 tagctagctgactgatcgatcg 107 22 0 50.00 60.174 28.29 13.89 0.00 2.174 153 agctgatcatcatcgatgct 515 20 0 45.00 57.788 11.25 0.72 40.32 2.212 154 agctagctgactgatcgatcat 260 22 0 45.45 59.778 26.67 18.02 36.62 2.222 155 tgactgatacgcgatgctagc 474 21 0 52.38 61.238 8.61 8.61 0.00 2.238 156 gctgactgatacgcgatgcta 472 21 0 52.38 61.238 2.33 0.00 0.00 2.238 157 tagctgactgatacgcgatgc 470 21 0 52.38 61.238 3.47 0.00 0.00 2.238 158 tagctagctgatcgatcgtagc 561 22 0 50.00 60.238 26.87 26.87 0.00 2.238 159 gctagctagctgatcgatcgta 559 22 0 50.00 60.238 34.38 3.07 46.11 2.238 160 tgatcgatcgatgctagctagg 400 22 0 50.00 60.239 26.44 6.29 35.21 2.239 161 gctgactgatcgatcgatgct 113 21 0 52.38 61.244 26.44 12.40 35.21 2.244 162 agctgactgatcgatcgatgc 112 21 0 52.38 61.244 24.08 19.43 35.21 2.244 163 gatcgatcgatgtgcggct 81 19 0 57.89 61.263 19.16 0.00 41.65 2.263 164 gctgatcatcgatgctactagc 195 22 0 50.00 59.727 18.08 16.44 45.61 2.273 165 gctagctgatcatcgatgctac 191 22 0 50.00 59.727 9.57 5.47 0.00 2.273 166 gatcgatcgatgtgcggc 81 18 0 61.11 59.714 18.10 0.95 41.65 2.286 167 ctagctagctgactgatacgc 465 21 0 52.38 58.703 14.90 0.00 0.00 2.297 168 tagctgatcgatcgatgtgcg 76 21 0 52.38 61.299 31.92 20.26 42.69 2.299 169 agctgatcgatcgatgtgc 77 19 0 52.63 58.698 31.83 30.04 35.21 2.302 170 gctgatcatcatcgatgctagc 516 22 0 50.00 60.302 10.80 10.34 40.32 2.302 171 gctagctgatcatcatcgatgc 512 22 0 50.00 60.302 21.42 7.40 40.32 2.302 172 aagcatcggattagctagctga 2 22 0 45.45 60.306 28.29 20.88 0.00 2.306 173 gatcgatcgtagcggcga 570 18 0 61.11 60.318 13.01 8.51 45.59 2.318 174 atcggattagctagctgatgc 6 21 0 47.62 58.673 17.46 17.46 40.05 2.327 175 gcatcggattagctagctgat 4 21 0 47.62 58.673 17.84 15.13 0.00 2.327 176 gcggcgatctagctagctg 581 19 0 63.16 61.329 17.07 8.17 38.05 2.329 177 tgctagtgatgcatgctagt 24 20 0 45.00 57.636 24.96 11.17 35.89 2.364 178 ctactatcatctctgcgcga 357 20 0 50.00 57.636 2.71 2.71 0.00 2.364 179 actagctagctgactgatacgc 464 22 0 50.00 60.368 21.52 0.00 0.00 2.368 180 gctagctagctgatcatcga 187 20 0 50.00 57.613 34.38 0.24 46.11 2.387 181 tctctgcgcgatcgatgcta 366 20 0 55.00 62.413 26.61 17.10 38.48 2.413 182 tgatcgatcgatgctagctagt 119 22 0 45.45 59.586 26.44 5.69 35.21 2.414 183 actgatcgatcgatgctagcta 117 22 0 45.45 59.586 23.29 7.65 35.21 2.414 184 tagctagctgatcgatcgatgt 72 22 0 45.45 59.586 14.29 2.62 35.21 2.414 185 agctaggcgatgctagcta 415 19 0 52.63 58.572 17.69 3.08 41.54 2.428 186 tagctaggcgatgctagct 414 19 0 52.63 58.572 17.35 17.35 43.53 2.428 187 gatcgatcgatgctagctagg 401 21 0 52.38 58.567 23.89 6.29 35.21 2.433 188 gctagctagctgactgatcgat 105 22 0 50.00 60.434 34.38 0.00 46.11 2.434 189 gatcgatgctagctaggcg 405 19 0 57.89 58.563 15.59 9.02 0.00 2.437 190 cgatcgatgctagctaggc 404 19 0 57.89 58.563 14.87 8.45 0.00 2.437 191 tagctagctgactgatacgc 466 20 0 50.00 57.549 28.29 0.00 0.00 2.451 192 agctagctgactgatcgatc 260 20 0 50.00 57.536 19.32 19.32 0.00 2.464 193 agctagctgactgatcgatc 108 20 0 50.00 57.536 19.32 19.32 0.00 2.464 194 aaagcatcggattagctagctg 1 22 0 45.45 59.524 28.29 10.80 0.00 2.476 195 gctgactgatcgatcatcatgc 265 22 0 50.00 60.493 25.66 25.12 41.77 2.493 196 tagctactatcatctctgcgcg 354 22 0 50.00 60.495 0.00 0.00 0.00 2.495 197 ctgatcgatcgatgtgcggc 79 20 0 60.00 62.497 15.54 4.15 41.65 2.497 198 gctgatcgatcgatgtgcgg 78 20 0 60.00 62.497 29.87 22.23 41.65 2.497 199 tcgtagcggcgatctagct 576 19 0 57.89 61.503 11.03 11.03 36.40 2.503 200 agcggcgatctagctagct 580 19 0 57.89 61.519 23.74 23.74 42.96 2.519 201 ctagctagctgatcatcgatgc 188 22 0 50.00 59.470 21.42 9.22 0.00 2.530 202 gctagctagctgatcatcgatg 187 22 0 50.00 59.470 34.38 20.23 46.11 2.530 203 tcatctctgcgcgatcga 363 18 0 55.56 59.468 22.07 0.00 38.48 2.532 204 tcgatcgatgtgcggcta 83 18 0 55.56 59.465 18.63 0.00 41.65 2.535 205 tgatcgatcgatgtgcggc 80 19 0 57.89 61.549 21.27 8.44 41.65 2.549 206 ctagctaggcgatgctagctag 413 22 0 54.55 60.561 22.99 22.99 46.84 2.561 207 gctagctagctgatcgatcgat 390 22 0 50.00 60.561 34.38 30.53 46.11 2.561 208 gctagctagctgatcgatcgat 70 22 0 50.00 60.561 34.38 30.53 46.11 2.561 209 ctgcgcgatcgatgctag 369 18 0 61.11 59.415 19.79 13.97 38.48 2.585 210 aagcatcggattagctagct 2 20 0 45.00 57.413 23.74 23.74 0.00 2.587 211 gctagctgatcatcgatgcta 191 21 0 47.62 58.410 16.61 13.09 0.00 2.590 212 tagctagctgatcatcgatgc 189 21 0 47.62 58.410 21.42 9.22 0.00 2.590 213 actgatacgcgatgctagc 476 19 0 52.63 58.407 8.61 8.61 0.00 2.593 214 gctagctgactgatcgatcatc 261 22 0 50.00 59.406 23.93 21.92 36.62 2.594 215 atcgatcgatgctagctagg 402 20 0 50.00 57.396 29.65 6.29 33.56 2.604 216 atctctgcgcgatcgatgc 365 19 0 57.89 61.618 22.01 22.01 38.48 2.618 217 tgatcgatcgtagcggcg 569 18 0 61.11 60.621 20.58 0.86 0.00 2.621 218 atcatctctgcgcgatcgatg 362 21 0 52.38 61.633 18.01 18.01 38.48 2.633 219 agctagctgatcgatcgtag 562 20 0 50.00 57.344 17.99 16.86 0.00 2.656 220 ctagctagctgatcgatcgt 560 20 0 50.00 57.344 16.40 2.65 0.00 2.656 221 gctagctgactgatcgatcat 261 21 0 47.62 58.339 26.67 18.02 36.62 2.661 222 ctgatcgatcgtagcggcg 568 19 0 63.16 61.664 14.21 0.86 0.00 2.664 223 ctgactgatacgcgatgct 473 19 0 52.63 58.330 2.33 0.00 0.00 2.670 224 agctgactgatacgcgatg 471 19 0 52.63 58.330 0.00 0.00 0.00 2.670 225 gctagctgatcgatcgtagcg 563 21 0 57.14 61.676 24.18 16.49 0.00 2.676 226 ctagctgactgatcgatcga 110 20 0 50.00 57.276 22.52 22.52 0.00 2.724 227 agctagctactgatcgatgcta 303 22 0 45.45 59.252 17.56 12.23 0.00 2.748 228 tagctagctactgatcgatgct 302 22 0 45.45 59.252 27.80 11.51 0.00 2.748 229 gactgatacgcgatgctagcta 475 22 0 50.00 60.751 17.69 3.08 0.00 2.751 230 actgatacgcgatgctagctag 476 22 0 50.00 60.752 17.46 17.46 0.00 2.752 231 gactgatcgatcgatgctagct 116 22 0 50.00 60.753 18.04 9.56 35.21 2.753 232 gctagctgactgatcgatcgat 109 22 0 50.00 60.753 30.53 30.53 37.90 2.753 233 tgcgcgatcgatgctagcta 370 20 0 55.00 62.756 26.61 9.85 38.48 2.756 234 gcggcgatctagctagctga 581 20 0 60.00 62.765 21.57 16.66 38.70 2.765 235 agcggcgatctagctagctg 580 20 0 60.00 62.771 17.07 8.17 44.87 2.771 236 ctactatcatctctgcgcgatc 357 22 0 50.00 59.220 4.99 0.00 0.00 2.780 237 tatcatctctgcgcgatcg 361 19 0 52.63 58.199 12.19 12.19 39.07 2.801 238 tactatcatctctgcgcgatcg 358 22 0 50.00 60.811 12.19 12.19 39.07 2.811 239 tagctagctgactgatcgatca 259 22 0 45.45 59.187 28.29 26.99 35.44 2.813 240 gctgatcgatcgatgctagcta 398 22 0 50.00 60.816 27.88 12.57 35.21 2.816 241 tagctgatcgatcgatgctagc 396 22 0 50.00 60.816 30.84 27.90 36.80 2.816 242 gctagctgatcgatcgatgcta 394 22 0 50.00 60.816 34.11 32.56 36.80 2.816 243 tagctagctgatcgatcgatgc 392 22 0 50.00 60.816 24.08 21.09 35.21 2.816 244 gctaggcgatgctagctag 416 19 0 57.89 58.179 17.46 17.46 35.42 2.821 245 ctagctaggcgatgctagc 413 19 0 57.89 58.179 18.68 9.44 36.95 2.821 246 gctagctaggcgatgctag 412 19 0 57.89 58.179 23.16 23.16 38.59 2.821 247 catctctgcgcgatcgatgc 364 20 0 60.00 62.823 22.01 22.01 38.48 2.823 248 aaagcatcggattagctagct 1 21 0 42.86 58.168 23.74 23.74 0.00 2.832 249 ctgactgatcgatcgatgctag 114 22 0 50.00 59.155 22.81 19.44 35.21 2.845 250 ctagctgactgatcgatcgatg 110 22 0 50.00 59.155 23.05 13.21 35.21 2.845 251 tactatcatctctgcgcgatc 358 21 0 47.62 58.155 4.99 0.00 0.00 2.845 252 gctagctactgatcgatgctac 304 22 0 50.00 59.151 8.57 4.26 0.00 2.849 253 ctactatcatctctgcgcgat 357 21 0 47.62 58.150 4.99 2.52 0.00 2.850 254 agctgatcatcgatgctact 194 20 0 45.00 57.134 17.14 8.95 0.00 2.866 255 gctagctagctgatcatcgat 187 21 0 47.62 58.132 34.38 0.00 46.11 2.868 256 ctgatacgcgatgctagct 477 19 0 52.63 58.107 17.69 1.35 0.00 2.893 257 ggcgatctagctagctgac 583 19 0 57.89 58.106 14.84 7.25 37.58 2.894 258 tgactgatcgatcgatgctag 115 21 0 47.62 58.084 22.81 19.44 35.21 2.916 259 ctgactgatcgatcgatgcta 114 21 0 47.62 58.084 26.44 14.64 35.21 2.916 260 tagctgactgatcgatcgatg 111 21 0 47.62 58.084 23.05 13.21 35.21 2.916 261 actgatcgatcatcatgctagc 269 22 0 45.45 59.071 26.67 8.61 41.77 2.929 262 agctgactgatcgatcatca 264 20 0 45.00 57.064 26.49 26.49 39.98 2.936 263 ctagctagctgatcgatcga 391 20 0 50.00 57.060 22.52 22.52 0.00 2.940 264 ctagctagctgatcgatcga 71 20 0 50.00 57.060 22.52 22.52 0.00 2.940 265 gatcgatcgatgtgcggctag 81 21 0 57.14 61.947 19.16 0.00 41.65 2.947 266 tgctagctaggcgatgct 411 18 0 55.56 59.050 17.69 7.53 0.00 2.950 267 gctgatcgatcgtagcggc 567 19 0 63.16 61.960 20.49 19.38 0.00 2.960 268 cgatcgatgtgcggctag 84 18 0 61.11 59.032 10.82 0.00 41.65 2.968 269 tgatcgatcgatgtgcggct 80 20 0 55.00 62.987 22.21 0.00 41.65 2.987 270 ctgactgatcgatcatcatgct 266 22 0 45.45 59.004 20.99 6.82 41.77 2.996 271 agctgactgatcgatcatcatg 264 22 0 45.45 59.004 22.70 19.51 41.77 2.996 272 agctagctgactgatacgcga 467 21 0 52.38 62.000 17.99 0.00 0.00 3.000 273 tgactgatcgatcgatgctagc 115 22 0 50.00 61.008 18.17 12.52 35.21 3.008 274 gctgactgatcgatcgatgcta 113 22 0 50.00 61.008 26.44 14.64 35.21 3.008 275 tagctgactgatcgatcgatgc 111 22 0 50.00 61.008 24.08 19.43 35.21 3.008 276 agctagctgatcgatcgatgtg 73 22 0 50.00 61.010 17.99 6.93 35.21 3.010 277 ctgatcgatcgatgctagctag 399 22 0 50.00 58.963 19.70 17.46 35.21 3.037 278 ctagctgatcgatcgatgctag 395 22 0 50.00 58.963 37.86 37.86 43.17 3.037 279 ctagctagctgatcgatcgatg 391 22 0 50.00 58.963 21.99 11.03 35.21 3.037 280 ctgatcgatcgatgctagctag 118 22 0 50.00 58.963 19.70 17.46 35.21 3.037 281 ctagctagctgatcgatcgatg 71 22 0 50.00 58.963 21.99 11.03 35.21 3.037 282 gactgatcgatcatcatgctagc 268 23 0 47.83 60.053 24.51 9.03 41.77 3.053 283 gctagtgatgcatgctagtagtg 25 23 0 47.83 59.929 24.96 10.54 0.00 3.071 284 gctactatcatctctgcgcgat 356 22 0 50.00 61.073 4.99 2.52 0.00 3.073 285 gtagcggcgatctagctag 578 19 0 57.89 57.903 15.64 15.64 37.58 3.097 286 tgactgatcgatcatcatgct 267 21 0 42.86 57.900 26.79 12.28 41.77 3.100 287 ctagctactatcatctctgcgc 353 22 0 50.00 58.892 0.00 0.00 0.00 3.108 288 gctagctactatcatctctgcg 352 22 0 50.00 58.892 8.21 0.00 0.00 3.108 289 ctagctagctactgatcgatgc 301 22 0 50.00 58.892 14.00 1.76 0.00 3.108 290 gctagctagctactgatcgatg 300 22 0 50.00 58.892 34.38 7.78 46.11 3.108 291 catcgatcgatgctagtatgct 325 22 0 45.45 58.885 37.80 10.02 44.93 3.115 292 tgatcgatcgatgctagctag 400 21 0 47.62 57.879 26.44 17.46 35.21 3.121 293 ctgatcgatcgatgctagcta 399 21 0 47.62 57.879 19.70 3.77 35.21 3.121 294 tagctgatcgatcgatgctag 396 21 0 47.62 57.879 34.03 27.12 36.80 3.121 295 ctagctgatcgatcgatgcta 395 21 0 47.62 57.879 34.11 32.56 36.80 3.121 296 tagctagctgatcgatcgatg 392 21 0 47.62 57.879 21.99 11.03 35.21 3.121 297 tgatcgatcgatgctagctag 119 21 0 47.62 57.879 26.44 17.46 35.21 3.121 298 ctgatcgatcgatgctagcta 118 21 0 47.62 57.879 19.70 3.77 35.21 3.121 299 tagctagctgatcgatcgatg 72 21 0 47.62 57.879 21.99 11.03 35.21 3.121 300 gatcgatcgatgctagctagt 120 21 0 47.62 57.878 23.89 3.56 35.21 3.122 301 ctatcatctctgcgcgatcgat 360 22 0 50.00 61.132 22.07 11.47 38.48 3.132 302 tgatcgatcgtagcggcga 569 19 0 57.89 62.144 20.58 8.51 45.59 3.144 303 tcgtagcggcgatctagctag 576 21 0 57.14 62.173 15.64 15.64 36.40 3.173 304 tactagctagctgactgatacgc 463 23 0 47.83 60.176 13.17 0.00 0.00 3.176 305 ctgatcatcatcgatgctagct 517 22 0 45.45 58.807 17.69 1.76 40.32 3.193 306 agctgatcatcatcgatgctag 515 22 0 45.45 58.807 5.93 2.76 40.32 3.193 307 ctagctgatcatcatcgatgct 513 22 0 45.45 58.807 14.14 8.34 40.32 3.193 308 agctagctgatcatcatcgatg 511 22 0 45.45 58.807 20.23 20.23 41.48 3.193 309 ctgatcgatcatcatgctagct 270 22 0 45.45 58.807 26.67 0.00 41.77 3.193 310 ctagctgactgatcgatcgat 110 21 0 47.62 57.806 30.53 30.53 37.90 3.194 311 ttagctagctgactgatcgatca 258 23 0 43.48 59.798 28.29 26.99 35.44 3.202 312 tagctactatcatctctgcgc 354 21 0 47.62 57.798 0.00 0.00 0.00 3.202 313 gctagctactgatcgatgcta 304 21 0 47.62 57.798 12.99 12.23 0.00 3.202 314 tagctagctactgatcgatgc 302 21 0 47.62 57.798 27.80 1.76 0.00 3.202 315 tctctgcgcgatcgatgc 366 18 0 61.11 61.229 22.01 22.01 38.48 3.229 316 ctctgcgcgatcgatgct 367 18 0 61.11 61.232 26.61 0.00 38.48 3.232 317 agctagctactatcatctctgcg 351 23 0 47.83 60.238 17.56 0.00 0.00 3.238 318 ctagctagctactgatcgatgct 301 23 0 47.83 60.238 14.00 11.51 0.00 3.238 319 ctgatcgatcgtagcggc 568 18 0 61.11 58.727 14.21 0.00 0.00 3.273 320 gctgatcgatcgtagcgg 567 18 0 61.11 58.727 20.49 15.03 0.00 3.273 321 agctaggcgatgctagct 415 18 0 55.56 58.725 17.69 13.00 39.84 3.275 322 tgctagtgatgcatgctagtagt 24 23 0 43.48 60.302 24.96 12.98 35.89 3.302 323 gcgcgatcgatgctagct 371 18 0 61.11 61.306 21.66 1.35 38.48 3.306 324 tgatcatcatcgatgctagct 518 21 0 42.86 57.691 17.69 1.76 40.32 3.309 325 agctgatcatcatcgatgcta 515 21 0 42.86 57.691 0.24 0.00 40.32 3.309 326 tagctgatcatcatcgatgct 514 21 0 42.86 57.691 14.14 8.34 40.32 3.309 327 tgatcgatcatcatgctagct 271 21 0 42.86 57.691 27.48 0.00 37.38 3.309 328 ctagctagctgactgatacgcg 465 22 0 54.55 61.313 14.90 3.89 0.00 3.313 329 tgctagtgatgcatgctagtag 24 22 0 45.45 58.673 24.96 19.76 35.89 3.327 330 gctagtgatgcatgctagtagt 25 22 0 45.45 58.672 24.96 12.98 0.00 3.328 331 tagctagctgatcatcgatgcta 189 23 0 43.48 59.671 17.82 15.89 0.00 3.329 332 agctagctgactgatacgc 467 19 0 52.63 57.642 17.99 0.00 0.00 3.358 333 tatcatctctgcgcgatcgatg 361 22 0 50.00 61.383 18.01 18.01 38.48 3.383 334 agctgactgatcgatcatcat 264 21 0 42.86 57.616 26.31 18.90 41.77 3.384 335 actagctagctgatcatcatcga 508 23 0 43.48 59.607 22.43 0.00 0.00 3.393 336 tagctagctgactgatcgatcat 259 23 0 43.48 59.607 28.29 18.02 36.62 3.393 337 gatgctagctaggcgatgctag 409 22 0 54.55 61.393 23.16 23.16 38.59 3.393 338 cggcgatctagctagctgact 582 21 0 57.14 62.394 17.44 7.44 38.70 3.394 339 ctagctagctgatcgatcgat 391 21 0 47.62 57.600 30.53 30.53 37.90 3.400 340 ctagctagctgatcgatcgat 71 21 0 47.62 57.600 30.53 30.53 37.90 3.400 341 actgatcgatcatcatgctagct 269 23 0 43.48 60.428 26.67 4.70 41.77 3.428 342 ctagctagctgactgatcgatc 106 22 0 50.00 58.567 19.32 19.32 0.00 3.433 343 ctgactgatcgatcatcatgc 266 21 0 47.62 57.562 20.99 12.19 41.77 3.438 344 gctgactgatcgatcatcatg 265 21 0 47.62 57.562 22.70 19.51 41.77 3.438 345 gatcgatcgatgctagctaggc 401 22 0 54.55 61.448 23.89 8.45 35.21 3.448 346 gtgatgcatgctagtagtgatgt 29 23 0 43.48 59.551 11.60 0.00 0.00 3.449 347 tgctagtgatgcatgctagta 24 21 0 42.86 57.546 24.96 21.25 35.89 3.454 348 tagcggcgatctagctagctg 579 21 0 57.14 62.457 18.98 9.30 45.57 3.457 349 gtagcggcgatctagctagct 578 21 0 57.14 62.458 23.74 23.74 46.60 3.458 350 tagctgatcgatcgtagcg 565 19 0 52.63 57.539 25.02 11.96 0.00 3.461 351 gctagctagctactgatcgat 300 21 0 47.62 57.517 34.38 0.00 46.11 3.483 352 agctagctactgatcgatgctac 303 23 0 47.83 60.487 17.56 7.81 0.00 3.487 353 atcgatcgatgctagtatgct 326 21 0 42.86 57.502 29.65 2.17 33.56 3.498 354 agctactgatcgatgctacatc 307 22 0 45.45 58.484 7.41 0.00 37.97 3.516 355 agtgatgcatgctagtagtga 28 21 0 42.86 57.471 0.00 0.00 0.00 3.529 356 gactgatcgatcgatgctagcta 116 23 0 47.83 60.546 18.04 4.19 35.21 3.546 357 ctgatcgatcgatgctagctagt 118 23 0 47.83 60.547 22.18 3.56 35.21 3.547 358 actgatcgatcgatgctagctag 117 23 0 47.83 60.547 23.29 17.46 35.21 3.547 359 ctagctagctgatcgatcgatgt 71 23 0 47.83 60.547 14.29 2.62 35.21 3.547 360 catcgatcgatgctagtatgc 325 21 0 47.62 57.452 37.80 0.00 44.93 3.548 361 tagctagctgactgatcgatc 259 21 0 47.62 57.451 28.29 19.32 0.00 3.549 362 tagctagctgactgatcgatc 107 21 0 47.62 57.451 28.29 19.32 0.00 3.549 363 ctagctagctgactgatcgat 106 21 0 47.62 57.445 14.90 0.00 0.00 3.555 364 tcgatgctagctaggcga 407 18 0 55.56 58.427 15.59 13.52 0.00 3.573 365 tgatcgatcgatgctagctagta 119 23 0 43.48 59.424 26.44 18.77 35.21 3.576 366 ctgcgcgatcgatgctagc 369 19 0 63.16 62.586 20.26 12.12 38.48 3.586 367 agctagctgatcatcatcgat 511 21 0 42.86 57.405 17.99 0.00 0.00 3.595 368 tgcgcgatcgatgctagc 370 18 0 61.11 61.605 26.61 17.77 38.48 3.605 369 ctagctagctgatcgatcgtag 560 22 0 50.00 58.387 16.86 16.86 0.00 3.613 370 actagctagctgactgatacg 464 21 0 47.62 57.384 21.52 2.77 0.00 3.616 371 ctagctgactgatacgcga 469 19 0 52.63 57.370 16.29 0.00 0.00 3.630 372 gctactagctagctgactgat 461 21 0 47.62 57.360 15.96 3.00 44.92 3.640 373 ctgatcatcatcgatgctagc 517 21 0 47.62 57.358 8.61 8.61 40.32 3.642 374 gctgatcatcatcgatgctag 516 21 0 47.62 57.358 5.93 2.76 40.32 3.642 375 ctagctgatcatcatcgatgc 513 21 0 47.62 57.358 21.42 7.40 40.32 3.642 376 gctagctgatcatcatcgatg 512 21 0 47.62 57.358 20.23 20.23 41.48 3.642 377 ctgatcgatcatcatgctagc 270 21 0 47.62 57.358 26.67 8.61 41.77 3.642 378 agctactgatcgatgctacat 307 21 0 42.86 57.344 7.41 0.00 0.00 3.656 379 tgatcgatcgatgtgcggcta 80 21 0 52.38 62.663 22.21 0.24 41.65 3.663 380 atctctgcgcgatcgatg 365 18 0 55.56 58.327 12.03 4.19 38.48 3.673 381 catctctgcgcgatcgat 364 18 0 55.56 58.327 22.07 1.26 38.48 3.673 382 atcatctctgcgcgatcg 362 18 0 55.56 58.327 12.19 12.19 39.07 3.673 383 aagcatcggattagctagctgat 2 23 0 43.48 60.682 17.84 15.13 0.00 3.682 384 agtgatgcatgctagtagtgatg 28 23 0 43.48 59.299 7.49 7.49 0.00 3.701 385 gatcgatgctagctaggcgatg 405 22 0 54.55 61.702 24.26 6.24 0.00 3.702 386 atctctgcgcgatcgatgcta 365 21 0 52.38 62.723 26.61 17.10 38.48 3.723 387 tagctagctgactgatacgcga 466 22 0 50.00 61.730 28.29 0.00 0.00 3.730 388 tagctagctgatcgatcgtag 561 21 0 47.62 57.269 16.86 16.86 0.00 3.731 389 ctagctagctgatcgatcgta 560 21 0 47.62 57.269 11.68 3.07 0.00 3.731 390 tcgatcgatgctagctagtag 122 21 0 47.62 57.269 18.63 2.63 0.00 3.731 391 gctagctactgatcgatgctaca 304 23 0 47.83 60.734 11.21 0.06 0.00 3.734 392 agctagctgactgatcgatcatc 260 23 0 47.83 60.736 23.93 21.92 36.62 3.736 393 agctagctgactgatcgatcga 108 22 0 50.00 61.737 22.52 22.52 0.00 3.737 394 tctctgcgcgatcgatgct 366 19 0 57.89 62.740 26.61 0.00 38.48 3.740 395 tgatgcatgctagtagtgatgt 30 22 0 40.91 58.256 11.60 0.00 0.00 3.744 396 tgatcgatcgatgtgcgg 80 18 0 55.56 58.244 21.27 0.00 41.65 3.756 397 cgatcgatgctagctaggcga 404 21 0 57.14 62.770 16.30 16.30 0.00 3.770 398 tcgatcgatgctagctaggcg 403 21 0 57.14 62.770 18.63 11.67 0.00 3.770 399 atcgatcgatgcatgcatg 443 19 0 47.37 57.226 29.65 23.31 37.30 3.774 400 catcgatcgatgcatgcat 442 19 0 47.37 57.226 37.80 33.45 44.93 3.774 401 actagctagctgatcatcatcg 508 22 0 45.45 58.219 22.43 3.40 0.00 3.781 402 ctgatcatcgatgctactagct 196 22 0 45.45 58.219 10.86 0.00 45.92 3.781 403 agctgatcatcgatgctactag 194 22 0 45.45 58.219 13.67 9.68 0.00 3.781 404 ctagctgatcatcgatgctact 192 22 0 45.45 58.219 14.85 7.80 0.00 3.781 405 tttagctagctgactgatcga 257 21 0 42.86 57.216 0.38 0.00 0.00 3.784 406 tagctagctgatcgatcgatgtg 72 23 0 47.83 60.793 10.14 4.94 35.21 3.793 407 gctagctagctgatcatcatct 146 22 0 45.45 58.203 34.38 0.00 46.11 3.797 408 ctagctagctgatcatcgatgct 188 23 0 47.83 60.799 16.29 15.30 0.00 3.799 409 agctagctactatcatcgatcga 429 23 0 43.48 59.170 22.52 22.52 0.00 3.830 410 ttagctagctgactgatcgatc 258 22 0 45.45 58.159 28.29 19.32 0.00 3.841 411 ctagctgactgatcgatcatca 262 22 0 45.45 58.155 26.49 26.49 39.98 3.845 412 aaagcatcggattagctagctga 1 23 0 43.48 60.870 28.29 20.88 0.00 3.870 413 agctgactgatacgcgatgct 471 21 0 52.38 62.876 7.57 2.13 0.00 3.876 414 tagctagctactgatcgatgcta 302 23 0 43.48 59.102 27.80 12.23 0.00 3.898 415 tcatcatcgatgctagctagt 521 21 0 42.86 57.067 22.18 3.56 40.32 3.933 416 tcgatcatcatgctagctact 274 21 0 42.86 57.067 0.00 0.00 0.00 3.933 417 tgatcatcgatgctactagct 197 21 0 42.86 57.067 17.14 0.00 45.92 3.933 418 agctgatcatcgatgctacta 194 21 0 42.86 57.067 17.14 4.89 0.00 3.933 419 tagctgatcatcgatgctact 193 21 0 42.86 57.067 14.85 7.80 0.00 3.933 420 tcgatcgatgctagtatgctag 327 22 0 45.45 58.044 18.63 13.77 46.09 3.956 421 gcgatctagctagctgact 584 19 0 52.63 57.034 17.44 7.44 0.00 3.966 422 gctactgatcgatgctacatc 308 21 0 47.62 57.028 2.44 0.00 37.97 3.972 423 cggcgatctagctagctg 582 18 0 61.11 58.018 17.07 8.17 37.58 3.982 424 gctagctgactgatcgatcatca 261 23 0 47.83 60.983 26.49 26.49 39.98 3.983 425 actatcatctctgcgcgat 359 19 0 47.37 57.015 4.99 2.52 0.00 3.985 426 catcggattagctagctgatg 5 21 0 47.62 57.003 23.69 23.29 0.00 3.997 427 tagctgactgatcgatcatca 263 21 0 42.86 57.000 26.49 26.49 39.98 4.000 428 actagctagctgatcatcatcgat 508 24 0 41.67 59.995 22.43 0.00 0.00 4.005 429 agtgatgcatgctagtagtgat 28 22 0 40.91 57.984 0.00 0.00 0.00 4.016 430 ctagctagctgatcatcatcga 509 22 0 45.45 57.958 11.68 0.00 0.00 4.042 431 gctgatcatcgatgctactagct 195 23 0 47.83 61.046 20.91 5.24 45.92 4.046 432 agctgatcatcgatgctactagc 194 23 0 47.83 61.046 20.78 20.78 45.61 4.046 433 gctagctgatcatcgatgctact 191 23 0 47.83 61.046 12.59 8.92 0.00 4.046 434 agctagctgatcatcgatgctac 190 23 0 47.83 61.046 17.99 7.99 0.00 4.046 435 tagctagctactatcatctctgcg 350 24 0 45.83 60.058 27.80 0.00 0.00 4.058 436 ctagctagctactgatcgatgcta 301 24 0 45.83 60.058 14.00 12.23 0.00 4.058 437 agctactatcatctctgcgcga 355 22 0 50.00 62.058 2.71 2.71 0.00 4.058 438 gctatttagctagctgactgatcg 253 24 0 45.83 60.060 7.27 0.00 46.11 4.060 439 tgatcgatcatcatgctagctac 271 23 0 43.48 58.931 27.48 0.00 37.38 4.069 440 gtgatgcatgctagtagtgatg 29 22 0 45.45 57.921 7.49 7.49 0.00 4.079 441 ctagctagctgactgatcgatcg 106 23 0 52.17 61.091 16.84 13.89 0.00 4.091 442 ctagctactgatcgatgctaca 305 22 0 45.45 57.898 13.79 1.61 0.00 4.102 443 cggcgatctagctagctgacta 582 22 0 54.55 62.109 17.44 5.40 38.70 4.109 444 atgctagctaggcgatgc 410 18 0 55.56 57.890 10.59 0.00 0.00 4.110 445 ctgatcgatcatcatgctagctac 270 24 0 45.83 59.882 26.67 0.00 41.77 4.118 446 gctactagctagctgatcatca 505 22 0 45.45 57.879 14.37 0.00 44.92 4.121 447 gctactagctagctgatcatca 208 22 0 45.45 57.879 14.37 0.00 44.92 4.121 448 ctgactgatacgcgatgctagc 473 22 0 54.55 62.128 8.61 8.61 0.00 4.128 449 gctgactgatacgcgatgctag 472 22 0 54.55 62.128 1.70 0.00 0.00 4.128 450 ctagctgactgatacgcgatgc 469 22 0 54.55 62.128 3.47 0.00 0.00 4.128 451 gctagctgactgatacgcgatg 468 22 0 54.55 62.128 8.21 0.00 0.00 4.128 452 tgactgatcgatcatcatgctag 267 23 0 43.48 58.866 26.79 18.40 41.77 4.134 453 ctgactgatcgatcatcatgcta 266 23 0 43.48 58.866 20.99 8.89 41.77 4.134 454 tagctgactgatcgatcatcatg 263 23 0 43.48 58.866 22.70 19.51 41.77 4.134 455 ctagctagctgatcgatcgtagc 560 23 0 52.17 61.151 26.87 26.87 0.00 4.151 456 gctagctagctgatcgatcgtag 559 23 0 52.17 61.151 34.38 16.86 46.11 4.151 457 ctgatcgatcgatgctagctagg 399 23 0 52.17 61.158 22.67 6.29 35.21 4.158 458 gatcgatcgatgctagctagtag 120 23 0 47.83 58.829 23.89 2.63 35.21 4.171 459 ttagctagctgactgatcgatcat 258 24 0 41.67 60.177 28.29 18.02 36.62 4.177 460 ctgactgatcgatcatcatgctag 266 24 0 45.83 59.822 20.99 12.80 41.77 4.178 461 ctagctgactgatcgatcatcatg 262 24 0 45.83 59.822 22.70 19.51 41.77 4.178 462 ctagctgatcgatcgatgtgcg 75 22 0 54.55 62.180 31.92 20.85 42.69 4.180 463 tttagctagctgactgatcgatc 257 23 0 43.48 58.807 19.32 19.32 0.00 4.193 464 tgactgatcgatcatcatgcta 267 22 0 40.91 57.803 26.79 13.11 41.77 4.197 465 gctagctactatcatcgatcga 430 22 0 45.45 57.783 22.52 22.52 0.00 4.217 466 gatcgatcgatgctagctagta 120 22 0 45.45 57.783 23.89 18.77 35.21 4.217 467 agctagctactatcatcgatcg 429 22 0 45.45 57.777 17.56 9.87 0.00 4.223 468 atcgatcgatgctagctagtag 121 22 0 45.45 57.777 29.65 2.63 33.56 4.223 469 tgatcatcatcgatgctagctagt 518 24 0 41.67 60.238 22.18 3.56 40.32 4.238 470 tgatcgatcatcatgctagctact 271 24 0 41.67 60.238 27.48 0.00 37.38 4.238 471 actgatcgatcatcatgctagcta 269 24 0 41.67 60.238 26.67 2.76 41.77 4.238 472 catcgatcgatgctagtatgcta 325 23 0 43.48 58.753 37.80 6.23 44.93 4.247 473 tttagctagctgactgatcgat 257 22 0 40.91 57.736 0.38 0.00 0.00 4.264 474 atttagctagctgactgatcga 256 22 0 40.91 57.736 4.16 0.00 0.00 4.264 475 cgcgatcgatgctagcta 372 18 0 55.56 57.717 22.07 3.08 38.48 4.283 476 catcgatcgatgctagtatgctag 325 24 0 45.83 59.708 37.80 16.01 44.93 4.292 477 tagctagctactgatcgatgctac 302 24 0 45.83 60.297 27.80 7.81 0.00 4.297 478 agcatcggattagctagctgatg 3 23 0 47.83 61.299 27.81 27.81 33.28 4.299 479 agctagctgactgatacgcgat 467 22 0 50.00 62.317 17.99 0.00 0.00 4.317 480 tgatcatcatcgatgctagctag 518 23 0 43.48 58.678 17.46 17.46 40.32 4.322 481 ctgatcatcatcgatgctagcta 517 23 0 43.48 58.678 17.69 2.41 40.32 4.322 482 tagctgatcatcatcgatgctag 514 23 0 43.48 58.678 9.90 2.76 40.32 4.322 483 ctagctgatcatcatcgatgcta 513 23 0 43.48 58.678 14.58 9.63 40.32 4.322 484 tagctagctgatcatcatcgatg 510 23 0 43.48 58.678 20.23 20.23 41.48 4.322 485 ctgatcgatcatcatgctagcta 270 23 0 43.48 58.678 26.67 2.76 41.77 4.322 486 gatcatcatcgatgctagctagt 519 23 0 43.48 58.677 22.18 3.56 40.32 4.323 487 gatcgatcatcatgctagctact 272 23 0 43.48 58.677 21.11 0.00 0.00 4.323 488 gctagctagctgactgatcgatc 105 23 0 52.17 61.341 34.38 19.32 46.11 4.341 489 tgatcgatcgatgctagctagtag 119 24 0 45.83 60.356 26.44 2.63 35.21 4.356 490 ctgatcgatcgatgctagctagta 118 24 0 45.83 60.356 22.18 18.77 35.21 4.356 491 ctgatcatcatcgatgctagctag 517 24 0 45.83 59.644 17.46 17.46 40.32 4.356 492 ctagctgatcatcatcgatgctag 513 24 0 45.83 59.644 19.54 18.30 42.07 4.356 493 ctagctagctgatcatcatcgatg 509 24 0 45.83 59.644 20.23 20.23 41.48 4.356 494 tttagctagctgactgatcgatca 257 24 0 41.67 60.358 26.99 26.99 35.44 4.358 495 actatcatctctgcgcgatcga 359 22 0 50.00 62.365 22.07 1.72 38.48 4.365 496 agctgatcgatcgtagcg 566 18 0 55.56 57.634 25.02 11.96 0.00 4.366 497 gctactagctagctgactgatac 461 23 0 47.83 58.626 15.96 1.42 44.92 4.374 498 agctgatcgatcgatgctagct 397 22 0 50.00 62.388 30.32 30.32 38.42 4.388 499 agctagctgatcgatcgatgct 393 22 0 50.00 62.388 33.87 33.38 38.16 4.388 500 ctagctgactgatcgatcatcat 262 23 0 43.48 58.612 26.31 20.43 41.77 4.388 501 agctagctactatcatctctgc 351 22 0 45.45 57.608 17.56 0.00 0.00 4.392 502 ctagctactatcatctctgcgcg 353 23 0 52.17 61.393 0.00 0.00 0.00 4.393 503 tgatcatcatcgatgctagcta 518 22 0 40.91 57.602 17.69 3.08 40.32 4.398 504 tagctgatcatcatcgatgcta 514 22 0 40.91 57.602 15.95 9.16 40.32 4.398 505 tgatcgatcatcatgctagcta 271 22 0 40.91 57.602 27.48 3.08 37.38 4.398 506 atcatcatcgatgctagctagt 520 22 0 40.91 57.595 22.18 3.56 40.32 4.405 507 atcgatcatcatgctagctact 273 22 0 40.91 57.595 0.00 0.00 0.00 4.405 508 agctagctactatcatcgatcgat 429 24 0 41.67 59.573 25.37 25.37 35.13 4.427 509 gctagctgatcgatcgatgtgc 74 22 0 54.55 62.443 31.83 30.04 35.21 4.443 510 tagctgactgatcgatcatcat 263 22 0 40.91 57.531 26.31 20.43 41.77 4.469 511 tagctagctgactgatcgatcga 107 23 0 47.83 61.487 28.29 22.52 0.00 4.487 512 atcgatcgatgctagtatgctag 326 23 0 43.48 58.501 29.65 13.77 46.09 4.499 513 ctagtgatgcatgctagtagtga 26 23 0 43.48 58.483 20.00 1.46 0.00 4.517 514 tagctagctgactgatcgatcatc 259 24 0 45.83 60.536 28.29 21.92 36.62 4.536 515 tactagctagctgatcatcatcga 507 24 0 41.67 59.449 0.00 0.00 0.00 4.551 516 gctagctagctactatcatcga 426 22 0 45.45 57.437 34.38 0.00 46.11 4.563 517 tgactgatacgcgatgctagct 474 22 0 50.00 62.569 17.69 1.35 0.00 4.569 518 agctgactgatacgcgatgcta 471 22 0 50.00 62.569 7.57 0.00 0.00 4.569 519 tagctgactgatacgcgatgct 470 22 0 50.00 62.569 10.87 8.44 0.00 4.569 520 atcgatcgatgctagtatgcta 326 22 0 40.91 57.423 29.65 0.00 33.56 4.577 521 ctagctagctgatcatcatcgat 509 23 0 43.48 58.422 11.68 0.00 0.00 4.578 522 agctgactgatcgatcgatgct 112 22 0 50.00 62.580 26.44 23.13 35.21 4.580 523 ctagctagctgatcatcgatgcta 188 24 0 45.83 60.596 16.78 15.81 0.00 4.596 524 gtgatgcatgctagtagtgatgta 29 24 0 41.67 59.398 11.60 8.84 0.00 4.602 525 gctgatcatcatcgatgctagct 516 23 0 47.83 61.603 20.15 8.14 40.32 4.603 526 agctgatcatcatcgatgctagc 515 23 0 47.83 61.603 20.04 19.26 40.32 4.603 527 gctagctgatcatcatcgatgct 512 23 0 47.83 61.603 14.14 8.34 40.32 4.603 528 agctagctgatcatcatcgatgc 511 23 0 47.83 61.603 21.42 7.40 40.32 4.603 529 tagtgatgcatgctagtagtga 27 22 0 40.91 57.391 0.01 0.00 0.00 4.609 530 ctactagctagctgactgatacg 462 23 0 47.83 58.387 3.12 0.00 0.00 4.613 531 tagctactgatcgatgctacatc 306 23 0 43.48 58.368 13.79 0.00 37.97 4.632 532 gactgatacgcgatgctagctag 475 23 0 52.17 61.633 17.46 17.46 0.00 4.633 533 ctagctactgatcgatgctacat 305 23 0 43.48 58.364 13.79 0.09 0.00 4.636 534 gctagctactatcatctctgcgc 352 23 0 52.17 61.644 8.21 0.00 0.00 4.644 535 gctagctagctactgatcgatgc 300 23 0 52.17 61.644 34.38 12.71 46.11 4.644 536 gctactagctagctgatcatcat 505 23 0 43.48 58.350 14.37 0.00 44.92 4.650 537 gctactagctagctgatcatcat 208 23 0 43.48 58.350 14.37 0.00 44.92 4.650 538 ctagctactgatcgatgctacatc 305 24 0 45.83 59.349 13.79 0.00 37.97 4.651 539 gctactagctagctgatcatcatc 505 24 0 45.83 59.343 14.37 0.00 44.92 4.657 540 gctactagctagctgatcatcatc 208 24 0 45.83 59.343 14.37 0.00 44.92 4.657 541 gctgatcatcatctagctagtagc 154 24 0 45.83 59.343 15.25 15.25 45.79 4.657 542 tagctagctgatcatcatcgat 510 22 0 40.91 57.329 10.14 0.00 0.00 4.671 543 ctactatcatctctgcgcgatcg 357 23 0 52.17 61.686 12.19 12.19 39.07 4.686 544 tactagctagctgactgatacg 463 22 0 45.45 57.310 13.17 0.00 0.00 4.690 545 gctgatcgatcgatgctagctag 398 23 0 52.17 61.697 27.88 18.22 35.21 4.697 546 ctagctgatcgatcgatgctagc 395 23 0 52.17 61.697 38.64 35.38 43.05 4.697 547 gctagctgatcgatcgatgctag 394 23 0 52.17 61.697 41.07 41.07 46.89 4.697 548 ctagctagctgatcgatcgatgc 391 23 0 52.17 61.697 24.08 21.09 35.21 4.697 549 gctagctagctgatcgatcgatg 390 23 0 52.17 61.697 34.38 11.03 46.11 4.697 550 gctagctagctgatcgatcgatg 70 23 0 52.17 61.697 34.38 11.03 46.11 4.697 551 gctactagctagctgactgata 461 22 0 45.45 57.286 15.96 3.69 44.92 4.714 552 gatcgatcatcatgctagctac 272 22 0 45.45 57.284 21.11 0.00 0.00 4.716 553 cgatgctagctaggcgat 408 18 0 55.56 57.277 10.81 3.01 0.00 4.723 554 atcgatgctagctaggcg 406 18 0 55.56 57.277 15.59 9.02 0.00 4.723 555 tagctactgatcgatgctacat 306 22 0 40.91 57.270 13.79 0.09 0.00 4.730 556 gctagctactatcatcgatcgat 430 23 0 43.48 58.251 25.37 25.37 35.13 4.749 557 tgcatgctagtagtgatgtatacg 33 24 0 41.67 59.224 20.79 0.00 0.00 4.776 558 gcatgctagtagtgatgtatacgt 34 24 0 41.67 59.223 11.60 0.00 0.00 4.777 559 atttagctagctgactgatcgatc 256 24 0 41.67 59.219 19.32 19.32 0.00 4.781 560 gactgatcgatcatcatgctag 268 22 0 45.45 57.215 24.51 15.87 41.77 4.785 561 atttagctagctgactgatcgat 256 23 0 39.13 58.215 4.16 0.00 0.00 4.785 562 gctgactgatcgatcatcatgct 265 23 0 47.83 61.788 27.72 15.10 41.77 4.788 563 agctgactgatcgatcatcatgc 264 23 0 47.83 61.788 27.74 27.74 41.77 4.788 564 tagctactatcatctctgcgcga 354 23 0 47.83 61.796 2.71 2.71 0.00 4.796 565 gctgatcatcgatgctactagcta 195 24 0 45.83 60.834 20.91 7.37 45.92 4.834 566 tagctgatcatcgatgctactagc 193 24 0 45.83 60.834 20.78 20.78 45.61 4.834 567 gctagctgatcatcgatgctacta 191 24 0 45.83 60.834 11.65 8.83 0.00 4.834 568 tagctagctgatcatcgatgctac 189 24 0 45.83 60.834 17.82 12.06 0.00 4.834 569 tagtgatgcatgctagtagtgatg 27 24 0 41.67 59.155 11.56 7.49 0.00 4.845 570 agtgatgcatgctagtagtgatgt 28 24 0 41.67 60.846 11.60 0.00 0.00 4.846 571 tgatgcatgctagtagtgatgta 30 23 0 39.13 58.147 11.60 8.84 0.00 4.853 572 ctgactgatcgatcgatgctagc 114 23 0 52.17 61.878 18.17 12.52 35.21 4.878 573 gctgactgatcgatcgatgctag 113 23 0 52.17 61.878 22.81 19.44 35.21 4.878 574 ctagctgactgatcgatcgatgc 110 23 0 52.17 61.878 24.08 19.43 35.21 4.878 575 gctagctgactgatcgatcgatg 109 23 0 52.17 61.878 23.05 13.21 35.21 4.878 576 tcatcatcgatgctagctagtag 521 23 0 43.48 58.114 2.81 0.00 40.32 4.886 577 tactagctagctgatcatcatcg 507 23 0 43.48 58.114 0.00 0.00 0.00 4.886 578 tcgatcatcatgctagctactag 274 23 0 43.48 58.114 0.00 0.00 37.62 4.886 579 tgatcatcgatgctactagctag 197 23 0 43.48 58.114 20.04 20.04 45.92 4.886 580 ctgatcatcgatgctactagcta 196 23 0 43.48 58.114 10.86 0.00 45.92 4.886 581 tagctgatcatcgatgctactag 193 23 0 43.48 58.114 13.67 9.68 0.00 4.886 582 ctagctgatcatcgatgctacta 192 23 0 43.48 58.114 11.65 8.83 0.00 4.886 583 ctactagctagctgatcatcatcg 506 24 0 45.83 59.110 1.08 0.00 0.00 4.890 584 ctgatcatcgatgctactagctag 196 24 0 45.83 59.110 20.04 20.04 45.92 4.890 585 ctagctgatcatcgatgctactag 192 24 0 45.83 59.110 15.68 15.68 0.00 4.890 586 gctagctagctgatcatcatctag 146 24 0 45.83 59.102 34.38 13.13 44.71 4.898 587 gctagctagctgatcatcatcta 146 23 0 43.48 58.097 34.38 0.00 46.11 4.903 588 ctagtgatgcatgctagtagtg 26 22 0 45.45 57.072 20.00 3.96 0.00 4.928 589 gctactatcatctctgcgcgatc 356 23 0 52.17 61.936 4.99 0.00 0.00 4.936 590 gctgatcgatcgatgtgc 78 18 0 55.56 57.052 29.71 26.38 35.21 4.948 591 tagctagctactatcatcgatcga 428 24 0 41.67 59.031 27.80 22.52 0.00 4.969 592 agctagctgatcgatcgtagcg 562 22 0 54.55 62.971 27.20 18.31 0.00 4.971 593 tgactgatacgcgatgct 474 18 0 50.00 57.028 2.33 0.00 0.00 4.972 594 gatcatcatcgatgctagctag 519 22 0 45.45 57.019 17.46 17.46 40.32 4.981 595 tcatcatcgatgctagctagta 521 22 0 40.91 57.005 22.18 18.77 40.32 4.995 596 tcgatcatcatgctagctacta 274 22 0 40.91 57.005 0.00 0.00 0.00 4.995 597 tgatcatcgatgctactagcta 197 22 0 40.91 57.005 17.14 1.01 45.92 4.995 598 tagctgatcatcgatgctacta 193 22 0 40.91 57.005 12.62 8.42 0.00 4.995 599 tagctagctgactgatacgcgat 466 23 0 47.83 62.044 28.29 0.00 0.00 5.044 600 ctagctagctactatcatcgatcga 427 25 0 44.00 59.949 27.80 22.52 0.00 5.051 601 agctagctgactgatcgatcgat 108 23 0 47.83 62.053 30.53 30.53 37.90 5.053 602 ctactagctagctgactgatacgc 462 24 0 50.00 61.062 3.61 0.00 0.00 5.062 603 gctactagctagctgactgatacg 461 24 0 50.00 61.062 14.37 0.00 44.92 5.062 604 tgatcatcatcgatgctagctagta 518 25 0 40.00 60.062 22.18 18.77 40.32 5.062 605 tgatcgatcatcatgctagctacta 271 25 0 40.00 60.062 27.48 0.00 37.38 5.062 606 ctagtgatgcatgctagtagtgatg 26 25 0 44.00 60.064 20.00 7.49 0.00 5.064 607 gctagctagctactatcatcgatc 426 24 0 45.83 58.932 34.38 4.20 46.11 5.068 608 gctagctactgatcgatgctacat 304 24 0 45.83 61.072 11.21 0.00 0.00 5.072 609 gctagctagctactatcatcgat 426 23 0 43.48 57.922 34.38 0.00 46.11 5.078 610 ctagtgatgcatgctagtagtgat 26 24 0 41.67 58.911 20.00 4.64 0.00 5.089 611 tactatcatctctgcgcgatcga 358 23 0 47.83 62.092 22.07 1.72 38.48 5.092 612 agctgatcgatcgatgctagcta 397 23 0 47.83 62.111 31.26 14.51 40.03 5.111 613 tagctgatcgatcgatgctagct 396 23 0 47.83 62.111 33.22 33.22 42.08 5.111 614 agctagctgatcgatcgatgcta 393 23 0 47.83 62.111 34.11 32.56 36.80 5.111 615 tagctagctgatcgatcgatgct 392 23 0 47.83 62.111 33.87 33.38 38.16 5.111 616 tagtgatgcatgctagtagtgat 27 23 0 39.13 57.886 11.56 0.00 0.00 5.114 617 tactagctagctgatcatcatcgat 507 25 0 40.00 59.828 0.00 0.00 0.00 5.172 618 gctagctagctgatcatcgatgc 187 23 0 52.17 62.193 34.38 9.22 46.11 5.193 619 gctagtgatgcatgctagtagtga 25 24 0 45.83 61.195 24.96 4.57 0.00 5.195 620 aaagcatcggattagctagctgat 1 24 0 41.67 61.209 17.84 15.13 0.00 5.209 621 gtgatgcatgctagtagtgatgtat 29 25 0 40.00 59.774 1.07 1.07 0.00 5.226 622 ctatcatctctgcgcgatcgatg 360 23 0 52.17 62.227 18.01 18.01 38.48 5.227 623 tgactgatacgcgatgctagcta 474 23 0 47.83 62.287 17.69 3.08 0.00 5.287 624 tagctgactgatacgcgatgcta 470 23 0 47.83 62.287 12.64 9.09 0.00 5.287 625 tgctagtagtgatgtatacgtagct 37 25 0 40.00 59.713 9.37 8.73 0.00 5.287 626 tgactgatcgatcgatgctagct 115 23 0 47.83 62.296 20.19 11.59 35.21 5.296 627 agctgactgatcgatcgatgcta 112 23 0 47.83 62.296 26.44 14.64 35.21 5.296 628 tagctgactgatcgatcgatgct 111 23 0 47.83 62.296 31.98 30.27 35.21 5.296 629 ctagctagctactatcatcgatcg 427 24 0 45.83 58.703 12.49 9.87 0.00 5.297 630 tagctagctactatcatcgatcg 428 23 0 43.48 57.691 27.80 9.87 0.00 5.309 631 gactgatcgatcatcatgctagct 268 24 0 45.83 61.313 24.51 7.81 41.77 5.313 632 gctagctgactgatcgatcatcat 261 24 0 45.83 61.313 26.31 20.43 41.77 5.313 633 ctatttagctagctgactgatcga 254 24 0 41.67 58.679 0.00 0.00 0.00 5.321 634 gcatgctagtagtgatgtatacg 34 23 0 43.48 57.659 11.60 0.00 0.00 5.341 635 tatttagctagctgactgatcga 255 23 0 39.13 57.650 0.00 0.00 0.00 5.350 636 ctactagctagctgatcatcatcga 506 25 0 44.00 60.352 1.08 0.00 0.00 5.352 637 agctactatcatctctgcgcgat 355 23 0 47.83 62.360 4.99 2.52 0.00 5.360 638 gctgatcatcatcgatgctagcta 516 24 0 45.83 61.370 20.15 8.49 40.32 5.370 639 tagctgatcatcatcgatgctagc 514 24 0 45.83 61.370 20.04 19.26 40.32 5.370 640 gctagctgatcatcatcgatgcta 512 24 0 45.83 61.370 14.58 9.63 40.32 5.370 641 tagctagctgatcatcatcgatgc 510 24 0 45.83 61.370 21.42 7.40 40.32 5.370 642 atgcatgctagtagtgatgtatacg 32 25 0 40.00 59.604 11.70 0.00 0.00 5.396 643 tgatgcatgctagtagtgatgtat 30 24 0 37.50 58.592 12.66 12.66 0.00 5.408 644 gactgatcgatcgatgctagctag 116 24 0 50.00 61.409 18.04 17.46 35.21 5.409 645 gctatttagctagctgactgatc 253 23 0 43.48 57.572 7.27 0.00 46.11 5.428 646 tgctagtgatgcatgctagtagtg 24 24 0 45.83 61.434 24.96 10.54 35.89 5.434 647 ctagctagctactatcatctctgc 349 24 0 45.83 58.562 27.80 0.00 0.00 5.438 648 gctagctagctactatcatctctg 348 24 0 45.83 58.562 34.38 6.79 46.11 5.438 649 gatcatcatcgatgctagctagta 519 24 0 41.67 58.557 22.18 18.77 40.32 5.443 650 gatcgatcatcatgctagctacta 272 24 0 41.67 58.557 21.11 0.00 0.00 5.443 651 atcatcatcgatgctagctagtag 520 24 0 41.67 58.554 2.13 0.00 40.32 5.446 652 atcgatcatcatgctagctactag 273 24 0 41.67 58.554 0.00 0.00 37.62 5.446 653 tagctagctactatcatctctgc 350 23 0 43.48 57.527 27.80 0.00 0.00 5.473 654 atcatcatcgatgctagctagta 520 23 0 39.13 57.514 22.18 18.77 40.32 5.486 655 atcgatcatcatgctagctacta 273 23 0 39.13 57.514 0.00 0.00 0.00 5.486 656 gatcatcatcgatgctagctagtag 519 25 0 44.00 59.494 0.21 0.00 40.32 5.506 657 gatcgatcatcatgctagctactag 272 25 0 44.00 59.494 21.11 0.00 37.62 5.506 658 actagctagctgatcatcatctact 211 25 0 40.00 59.471 22.43 6.99 0.00 5.529 659 tgactgatcgatcatcatgctagc 267 24 0 45.83 61.547 26.79 14.13 41.77 5.547 660 gctgactgatcgatcatcatgcta 265 24 0 45.83 61.547 27.72 16.42 41.77 5.547 661 tagctgactgatcgatcatcatgc 263 24 0 45.83 61.547 27.74 27.74 41.77 5.547 662 tgctagtagtgatgtatacgtagc 37 24 0 41.67 58.446 4.93 4.93 0.00 5.554 663 ctagctagctgactgatacgcga 465 23 0 52.17 62.571 14.90 0.00 0.00 5.571 664 tagctagctactatcatcgatcgat 428 25 0 40.00 59.423 27.80 25.37 35.13 5.577 665 gctactagctagctgatcatcatct 208 25 0 44.00 60.585 14.37 0.00 44.92 5.585 666 agctgatcatcatctagctagtagc 153 25 0 44.00 60.585 15.25 15.25 40.45 5.585 667 ctagctagctgatcgatcgatgtg 71 24 0 50.00 61.642 11.68 4.94 35.21 5.642 668 agtgatgcatgctagtagtgatgta 28 25 0 40.00 60.646 11.60 8.84 0.00 5.646 669 tagtgatgcatgctagtagtgatgt 27 25 0 40.00 60.646 11.60 0.00 0.00 5.646 670 actatcatctctgcgcgatcgat 359 23 0 47.83 62.652 22.07 11.47 38.48 5.652 671 ctatttagctagctgactgatcg 254 23 0 43.48 57.339 0.00 0.00 0.00 5.661 672 tagctagctgatcgatcgtagcg 561 23 0 52.17 62.677 27.20 18.31 0.00 5.677 673 gcatcggattagctagctgatgc 4 23 0 52.17 62.687 34.10 34.10 41.15 5.687 674 tgcatgctagtagtgatgtatacgt 33 25 0 40.00 60.700 20.79 0.00 0.00 5.700 675 tttagctagctgactgatcgatcat 257 25 0 40.00 60.702 26.67 18.02 36.62 5.702 676 atttagctagctgactgatcgatca 256 25 0 40.00 60.702 26.99 26.99 35.44 5.702 677 gctagctagctactatcatctct 348 23 0 43.48 57.266 34.38 0.00 46.11 5.734 678 aagcatcggattagctagctgatg 2 24 0 45.83 61.794 27.81 27.81 33.28 5.794 679 agtgatgtatacgtagctagtagc 44 24 0 41.67 58.201 15.25 15.25 45.79 5.799 680 gctagtagtgatgtatacgtagct 38 24 0 41.67 58.201 5.92 5.92 0.00 5.799 681 tagctagctgactgatcgatcgat 107 24 0 45.83 61.800 30.53 30.53 37.90 5.800 682 actagctagctgactgatacgcg 464 23 0 52.17 62.816 21.52 3.89 0.00 5.816 683 actagctagctgatcatcatctac 211 24 0 41.67 58.178 22.43 0.00 0.00 5.822 684 tagctgatcgatcgatgctagcta 396 24 0 45.83 61.857 34.98 33.55 41.05 5.857 685 tagctagctgatcgatcgatgcta 392 24 0 45.83 61.857 34.11 32.56 36.80 5.857 686 tatttagctagctgactgatcgat 255 24 0 37.50 58.112 1.68 0.00 0.00 5.888 687 gcatgctagtagtgatgtatacgta 34 25 0 40.00 59.089 11.60 0.00 0.00 5.911 688 tatttagctagctgactgatcgatc 255 25 0 40.00 59.084 19.32 19.32 0.00 5.916 689 ctatttagctagctgactgatcgat 254 25 0 40.00 59.082 0.00 0.00 0.00 5.918 690 ctagctagctactatcatctctgcg 349 25 0 48.00 60.920 27.80 5.75 0.00 5.920 691 tgatcgatcgatgctagctaggc 400 23 0 52.17 62.955 26.44 12.49 35.21 5.955 692 agctagctactgatcgatgctaca 303 24 0 45.83 61.988 17.56 3.03 0.00 5.988 693 tgactgatcgatcgatgctagcta 115 24 0 45.83 62.034 20.19 11.16 35.21 6.034 694 tagctgactgatcgatcgatgcta 111 24 0 45.83 62.034 33.43 30.74 35.21 6.034 695 catgctagtagtgatgtatacgtagc 35 26 0 42.31 59.961 4.93 4.93 0.00 6.039 696 gcatgctagtagtgatgtatacgtag 34 26 0 42.31 59.961 11.60 0.00 0.00 6.039 697 actgatcgatcgatgctagctagt 117 24 0 45.83 62.039 23.29 15.07 35.21 6.039 698 ctatttagctagctgactgatcgatc 254 26 0 42.31 59.960 19.32 19.32 0.00 6.040 699 ttagctagctgactgatcgatcatc 258 25 0 44.00 61.041 28.29 21.92 36.62 6.041 700 atgctagtagtgatgtatacgtagct 36 26 0 38.46 60.068 9.37 8.73 0.00 6.068 701 ctagctagctgatcatcatctact 212 24 0 41.67 57.930 11.68 6.99 0.00 6.070 702 ctactagctagctgatcatcatct 209 24 0 41.67 57.930 1.08 0.00 0.00 6.070 703 agctgatcatcatctagctagtag 153 24 0 41.67 57.930 7.49 3.73 40.45 6.070 704 ctagctgatcatcatctagctagt 151 24 0 41.67 57.930 21.70 5.56 46.15 6.070 705 tagctactatcatctctgcgcgat 354 24 0 45.83 62.096 4.99 2.52 0.00 6.096 706 gactgatcgatcatcatgctagcta 268 25 0 44.00 61.099 24.51 4.57 41.77 6.099 707 ctgatcatcatcgatgctagctagt 517 25 0 44.00 61.101 22.18 3.56 40.32 6.101 708 actagctagctgatcatcatcgatg 508 25 0 44.00 61.101 22.43 20.23 41.48 6.101 709 ctgatcgatcatcatgctagctact 270 25 0 44.00 61.101 26.67 0.00 41.77 6.101 710 atgctagtagtgatgtatacgtagc 36 25 0 40.00 58.855 4.93 4.93 0.00 6.145 711 ctagctagctactgatcgatgctac 301 25 0 48.00 61.145 14.00 7.81 0.00 6.145 712 gctagctagctactatcatctctgc 348 25 0 48.00 61.152 34.38 11.74 46.11 6.152 713 agctagctgatcatcatctactatca 214 26 0 38.46 59.840 17.99 0.00 0.00 6.160 714 ctgatcgatcgatgctagctagtag 118 25 0 48.00 61.197 19.70 5.49 35.21 6.197 715 agctagctgactgatcgatcatca 260 24 0 45.83 62.232 26.49 26.49 39.98 6.232 716 gctagctagctactatcatcgatcg 426 25 0 48.00 61.252 34.38 9.87 46.11 6.252 717 gctatttagctagctgactgatcga 253 25 0 44.00 61.268 7.27 0.00 46.11 6.268 718 ctagctagctactatcatcgatcgat 427 26 0 42.31 60.291 27.80 25.37 35.13 6.291 719 agctgatcatcgatgctactagct 194 24 0 45.83 62.294 24.11 23.60 45.92 6.294 720 agctagctgatcatcgatgctact 190 24 0 45.83 62.294 17.99 10.88 0.00 6.294 721 ctagctagctgactgatcgatcga 106 24 0 50.00 62.312 22.52 22.52 0.00 6.312 722 actgatcgatcatcatgctagctac 269 25 0 44.00 61.328 26.67 0.00 41.77 6.328 723 tgatgcatgctagtagtgatgtatac 30 26 0 38.46 59.623 9.19 6.43 0.00 6.377 724 gtgatgcatgctagtagtgatgtata 29 26 0 38.46 59.623 4.23 0.00 0.00 6.377 725 tactatcatctctgcgcgatcgat 358 24 0 45.83 62.379 22.07 11.47 38.48 6.379 726 gctagctgatcatcatctactatca 215 25 0 40.00 58.607 8.21 0.00 0.00 6.393 727 gctactagctagctgatcatcatcta 208 26 0 42.31 60.404 14.37 0.00 44.92 6.404 728 tagctgatcatcatctagctagtagc 152 26 0 42.31 60.404 15.25 15.25 41.48 6.404 729 tgctagtagtgatgtatacgtagcta 37 26 0 38.46 59.564 10.00 8.05 0.00 6.436 730 gatgcatgctagtagtgatgtatacg 31 26 0 42.31 60.453 7.40 0.00 0.00 6.453 731 tagtgatgcatgctagtagtgatgta 27 26 0 38.46 60.461 11.60 8.84 0.00 6.461 732 gctagtgatgcatgctagtagtgat 25 25 0 44.00 61.506 24.96 8.51 0.00 6.506 733 tgcatgctagtagtgatgtatacgta 33 26 0 38.46 60.515 20.79 0.00 0.00 6.515 734 tatttagctagctgactgatcgatca 255 26 0 38.46 60.516 26.99 26.99 35.44 6.516 735 tgatgcatgctagtagtgatgtata 30 25 0 36.00 58.479 4.13 0.00 0.00 6.521 736 tactagctagctgactgatacgcg 463 24 0 50.00 62.538 13.17 3.89 0.00 6.538 737 ctagctactatcatctctgcgcga 353 24 0 50.00 62.603 2.71 2.71 0.00 6.603 738 agctagctgatcatcatctactatc 214 25 0 40.00 58.369 17.99 0.00 0.00 6.631 739 agctagctgatcatcatctactat 214 24 0 37.50 57.345 17.99 0.00 0.00 6.655 740 gctactagctagctgatcatcatcg 505 25 0 48.00 61.656 14.37 0.00 44.92 6.656 741 gctgatcatcgatgctactagctag 195 25 0 48.00 61.656 20.91 20.04 45.92 6.656 742 ctagctgatcatcgatgctactagc 192 25 0 48.00 61.656 20.78 20.78 45.61 6.656 743 gctagctgatcatcgatgctactag 191 25 0 48.00 61.656 20.13 20.13 0.00 6.656 744 ctagctagctgatcatcgatgctac 188 25 0 48.00 61.656 17.82 12.06 0.00 6.656 745 actagctagctgatcatcatctacta 211 26 0 38.46 59.328 22.43 8.11 0.00 6.672 746 tactagctagctgatcatcatctact 210 26 0 38.46 59.328 6.99 6.99 0.00 6.672 747 ctactagctagctgatcatcatcgat 506 26 0 42.31 60.681 1.08 0.00 0.00 6.681 748 tagctagctactgatcgatgctaca 302 25 0 44.00 61.746 27.80 5.02 0.00 6.746 749 gtagtgatgtatacgtagctagtagc 42 26 0 42.31 59.249 15.25 15.25 45.79 6.751 750 actgatcgatcgatgctagctagta 117 25 0 44.00 61.797 23.29 18.77 35.21 6.797 751 gatgcatgctagtagtgatgtatac 31 25 0 40.00 58.175 20.79 1.70 0.00 6.825 752 agctgatcatcatcgatgctagct 515 24 0 45.83 62.835 23.00 22.41 41.74 6.835 753 agctagctgatcatcatcgatgct 511 24 0 45.83 62.835 17.99 8.34 40.32 6.835 754 ctagctagctgactgatacgcgat 465 24 0 50.00 62.838 14.90 0.00 0.00 6.838 755 atgcatgctagtagtgatgtatac 32 24 0 37.50 57.161 11.70 0.53 0.00 6.839 756 agctagctactatcatctctgcgc 351 24 0 50.00 62.858 17.56 0.00 0.00 6.858 757 gctagctagctactgatcgatgct 300 24 0 50.00 62.858 34.38 11.51 46.11 6.858 758 ctactatcatctctgcgcgatcga 357 24 0 50.00 62.878 22.07 1.72 38.48 6.878 759 gctagctactgatcgatgctacatc 304 25 0 48.00 61.879 11.21 4.40 37.97 6.879 760 tagtgatgtatacgtagctagtagc 43 25 0 40.00 58.104 15.25 15.25 45.79 6.896 761 gctagtagtgatgtatacgtagcta 38 25 0 40.00 58.104 7.24 5.34 0.00 6.896 762 tgatcatcatcgatgctagctagtag 518 26 0 42.31 60.902 14.93 0.00 40.32 6.902 763 ctgatcatcatcgatgctagctagta 517 26 0 42.31 60.902 22.18 18.77 40.32 6.902 764 tactagctagctgatcatcatcgatg 507 26 0 42.31 60.902 20.23 20.23 41.48 6.902 765 tgatcgatcatcatgctagctactag 271 26 0 42.31 60.902 27.48 0.00 37.38 6.902 766 ctgatcgatcatcatgctagctacta 270 26 0 42.31 60.902 26.67 1.37 41.77 6.902 767 agctgatcgatcgatgctagctag 397 24 0 50.00 62.904 31.26 19.90 40.03 6.904 768 ctagctgatcgatcgatgctagct 395 24 0 50.00 62.904 35.51 33.22 43.05 6.904 769 agctagctgatcgatcgatgctag 393 24 0 50.00 62.904 41.07 41.07 46.89 6.904 770 ctagctagctgatcgatcgatgct 391 24 0 50.00 62.904 33.87 33.38 38.16 6.904 771 tactagctagctgatcatcatctac 210 25 0 40.00 58.081 0.00 0.00 0.00 6.919 772 gctagctgatcatcatctactatc 215 24 0 41.67 57.062 8.21 0.00 0.00 6.938 773 agtgatgcatgctagtagtgatgtat 28 26 0 38.46 60.969 1.07 1.07 0.00 6.969 774 gctagtagtgatgtatacgtagctag 38 26 0 42.31 59.027 8.99 8.99 0.00 6.973 775 tagctagctgactgatcgatcatca 259 25 0 44.00 61.981 28.29 26.49 39.98 6.981 776 ctactagctagctgatcatcatctac 209 26 0 42.31 59.015 1.08 0.00 0.00 6.985 777 agtagtgatgtatacgtagctagt 41 24 0 37.50 57.012 8.68 8.68 0.00 6.988 778 gctagctgatcatcatctactatcat 215 26 0 38.46 59.001 8.21 0.00 0.00 6.999 779 agctgatcatcatctactatcatca 218 25 0 36.00 57.993 0.00 0.00 0.00 7.007 780 atgcatgctagtagtgatgtatacgt 32 26 0 38.46 61.018 11.70 0.00 0.00 7.018 781 atttagctagctgactgatcgatcat 256 26 0 38.46 61.022 26.67 18.02 36.62 7.022 782 agctgatcatcgatgctactagcta 194 25 0 44.00 62.040 24.59 9.79 45.92 7.040 783 tagctgatcatcgatgctactagct 193 25 0 44.00 62.040 27.00 27.00 45.92 7.040 784 agctagctgatcatcgatgctacta 190 25 0 44.00 62.040 17.99 11.59 0.00 7.040 785 tagctagctgatcatcgatgctact 189 25 0 44.00 62.040 17.55 15.53 0.00 7.040 786 atgctagtagtgatgtatacgtagcta 36 27 0 37.04 59.911 10.00 8.05 0.00 7.089 787 gctagctgatcatcatctactatcatc 215 27 0 40.74 59.861 8.21 0.00 0.00 7.139 788 ctagctagctgatcatcatctacta 212 25 0 40.00 57.842 11.68 8.11 0.00 7.158 789 ctactagctagctgatcatcatcta 209 25 0 40.00 57.842 1.08 0.00 0.00 7.158 790 tagctgatcatcatctagctagtag 152 25 0 40.00 57.842 13.40 3.73 41.48 7.158 791 ctagctgatcatcatctagctagta 151 25 0 40.00 57.842 21.70 7.13 46.15 7.158 792 gctgatcatcatcgatgctagctag 516 25 0 48.00 62.165 20.15 17.46 40.32 7.165 793 ctagctgatcatcatcgatgctagc 513 25 0 48.00 62.165 19.27 19.26 41.96 7.165 794 gctagctgatcatcatcgatgctag 512 25 0 48.00 62.165 23.70 23.70 45.51 7.165 795 ctagctagctgatcatcatcgatgc 509 25 0 48.00 62.165 21.42 7.40 40.32 7.165 796 agctagctgatcatcatctactatcat 214 27 0 37.04 60.181 17.99 0.00 0.00 7.181 797 ctactagctagctgatcatcatctact 209 27 0 40.74 60.181 6.99 6.99 0.00 7.181 798 ctagctgatcatcatctagctagtag 151 26 0 42.31 58.789 21.70 10.73 46.15 7.211 799 aaagcatcggattagctagctgatg 1 25 0 44.00 62.249 27.81 27.81 33.28 7.249 800 agctagctactgatcgatgctacat 303 25 0 44.00 62.272 17.56 6.16 0.00 7.272 801 tagctagctgatcatcatctactatca 213 27 0 37.04 59.689 8.31 0.00 0.00 7.311 802 actagctagctgatcatcatctactat 211 27 0 37.04 59.688 22.43 2.36 0.00 7.312 803 ctgactgatcgatcatcatgctagc 266 25 0 48.00 62.333 20.99 8.86 41.77 7.333 804 gctgactgatcgatcatcatgctag 265 25 0 48.00 62.333 27.72 21.73 41.77 7.333 805 ctagctgactgatcgatcatcatgc 262 25 0 48.00 62.333 27.74 27.74 41.77 7.333 806 gctagctgactgatcgatcatcatg 261 25 0 48.00 62.333 22.70 19.51 41.77 7.333 807 tgctagtagtgatgtatacgtagctag 37 27 0 40.74 60.395 8.99 8.99 0.00 7.395 808 agtagtgatgtatacgtagctagtagc 41 27 0 40.74 60.395 15.25 15.25 45.79 7.395 809 gctagtagtgatgtatacgtagctagt 38 27 0 40.74 60.395 15.55 15.55 0.00 7.395 810 ctagtgatgcatgctagtagtgatgt 26 26 0 42.31 61.460 20.00 0.00 0.00 7.460 811 gctgatcatcatctactatcatcatca 219 27 0 37.04 59.533 0.00 0.00 0.00 7.467 812 agctagctgactgatcgatcatcat 260 25 0 44.00 62.507 26.31 20.43 41.77 7.507 813 tttagctagctgactgatcgatcatc 257 26 0 42.31 61.507 23.93 21.92 36.62 7.507 814 catgctagtagtgatgtatacgtag 35 25 0 40.00 57.441 4.74 0.00 0.00 7.559 815 agctgatcatcatcgatgctagcta 515 25 0 44.00 62.561 23.50 10.90 43.19 7.561 816 tagctgatcatcatcgatgctagct 514 25 0 44.00 62.561 26.21 26.21 44.79 7.561 817 agctagctgatcatcatcgatgcta 511 25 0 44.00 62.561 17.99 9.63 40.32 7.561 818 tagctagctgatcatcatcgatgct 510 25 0 44.00 62.561 14.14 10.38 40.32 7.561 819 gctatttagctagctgactgatcgat 253 26 0 42.31 61.564 7.27 0.00 46.11 7.564 820 ctagctagctgactgatcgatcgat 106 25 0 48.00 62.579 30.53 30.53 37.90 7.579 821 agctgatcatcatctactatcatcat 218 26 0 34.62 58.415 0.00 0.00 0.00 7.585 822 tagctagctactatcatctctgcgc 350 25 0 48.00 62.587 27.80 0.00 0.00 7.587 823 gctagctagctactgatcgatgcta 300 25 0 48.00 62.587 34.38 12.23 46.11 7.587 824 tgctagtgatgcatgctagtagtga 24 25 0 44.00 62.622 24.96 13.57 35.89 7.622 825 tagctgatcgatcgatgctagctag 396 25 0 48.00 62.632 33.71 21.46 41.05 7.632 826 ctagctgatcgatcgatgctagcta 395 25 0 48.00 62.632 35.51 32.61 43.05 7.632 827 tagctagctgatcgatcgatgctag 392 25 0 48.00 62.632 41.07 41.07 46.89 7.632 828 ctagctagctgatcgatcgatgcta 391 25 0 48.00 62.632 34.11 32.56 36.80 7.632 829 gtgatgcatgctagtagtgatgtatac 29 27 0 40.74 60.659 9.56 7.17 0.00 7.659 830 agctgatcatcatctactatcatcatc 218 27 0 37.04 59.314 0.00 0.00 0.00 7.686 831 tagctagctgatcatcatctactat 213 25 0 36.00 57.279 8.31 0.00 0.00 7.721 832 tagctagctgatcatcatctactatc 213 26 0 38.46 58.269 8.31 0.00 0.00 7.731 833 tgactgatcgatcatcatgctagct 267 25 0 44.00 62.733 26.79 10.55 41.77 7.733 834 agctgactgatcgatcatcatgcta 264 25 0 44.00 62.733 30.60 18.10 41.77 7.733 835 tagctgactgatcgatcatcatgct 263 25 0 44.00 62.733 32.31 32.31 41.77 7.733 836 ctagctagctgatcatcatctactat 212 26 0 38.46 58.265 9.41 0.00 0.00 7.735 837 agtgatgcatgctagtagtgatgtata 28 27 0 37.04 60.779 4.23 0.00 0.00 7.779 838 tagtgatgcatgctagtagtgatgtat 27 27 0 37.04 60.779 11.56 0.00 0.00 7.779 839 tgactgatcgatcgatgctagctag 115 25 0 48.00 62.800 20.19 17.46 35.21 7.800 840 ctgactgatcgatcgatgctagcta 114 25 0 48.00 62.800 20.19 11.97 35.21 7.800 841 tagctgactgatcgatcgatgctag 111 25 0 48.00 62.800 31.45 26.34 35.21 7.800 842 ctagctgactgatcgatcgatgcta 110 25 0 48.00 62.800 31.54 29.68 35.21 7.800 843 tagctagctgactgatcgatcgatg 107 25 0 48.00 62.800 28.29 13.21 35.21 7.800 844 gactgatcgatcgatgctagctagt 116 25 0 48.00 62.802 22.18 12.64 35.21 7.802 845 tactagctagctgatcatcatctacta 210 27 0 37.04 59.196 8.79 8.11 0.00 7.804 846 tagctgatcatcgatgctactagcta 193 26 0 42.31 61.805 28.86 27.39 45.92 7.805 847 tagctagctgatcatcgatgctacta 189 26 0 42.31 61.805 18.93 16.03 0.00 7.805 848 atgcatgctagtagtgatgtatacgta 32 27 0 37.04 60.828 11.70 0.00 0.00 7.828 849 tatttagctagctgactgatcgatcat 255 27 0 37.04 60.831 26.67 18.02 36.62 7.831 850 ctagctagctgatcatcatctactatc 212 27 0 40.74 59.162 9.41 0.06 0.00 7.838 851 tagctactatcatctctgcgcgatc 354 25 0 48.00 62.854 0.00 0.00 0.00 7.854 852 gctgatcatcatctactatcatcat 219 25 0 36.00 57.142 0.00 0.00 0.00 7.858 853 ctagctactatcatctctgcgcgat 353 25 0 48.00 62.859 4.99 2.52 0.00 7.859 854 gctgatcatcatctactatcatcatc 219 26 0 38.46 58.126 0.00 0.00 0.00 7.874 855 tagctagctactgatcgatgctacat 302 26 0 42.31 62.028 27.80 3.08 0.00 8.028 856 agtagtgatgtatacgtagctagtag 41 26 0 38.46 57.950 9.92 1.30 0.00 8.050 857 ctagtagtgatgtatacgtagctagt 39 26 0 38.46 57.950 15.52 15.52 0.00 8.050 858 catgctagtagtgatgtatacgtagct 35 27 0 40.74 61.089 9.37 8.73 0.00 8.089 859 gactgatcgatcatcatgctagctac 268 26 0 46.15 62.094 24.51 8.36 41.77 8.094 860 tagctgatcatcatctactatcatca 217 26 0 34.62 57.906 0.00 0.00 0.00 8.094 861 ctagctgatcatcatctactatcatca 216 27 0 37.04 58.826 0.00 0.00 0.00 8.174 862 ctagctgatcatcatctagctagtagc 151 27 0 44.44 61.196 21.70 15.25 46.15 8.196 863 tagctagctgactgatcgatcatcat 259 26 0 42.31 62.255 28.29 20.43 41.77 8.255 864 ctagtgatgcatgctagtagtgatgta 26 27 0 40.74 61.255 20.00 8.84 0.00 8.255 865 tgcatgctagtagtgatgtatacgtag 33 27 0 40.74 61.300 20.79 0.00 0.00 8.300 866 ctatttagctagctgactgatcgatca 254 27 0 40.74 61.304 26.99 26.99 35.44 8.304 867 tagctgatcatcatcgatgctagcta 514 26 0 42.31 62.307 28.23 26.63 43.19 8.307 868 tagctagctgatcatcatcgatgcta 510 26 0 42.31 62.307 14.58 11.03 40.32 8.307 869 gctagctagctactatcatcgatcga 426 26 0 46.15 62.381 34.38 22.52 46.11 8.381 870 gctactagctagctgatcatcatctac 208 27 0 44.44 61.407 14.37 0.00 44.92 8.407 871 ttagctagctgactgatcgatcatca 258 26 0 42.31 62.416 28.29 26.49 39.98 8.416 872 tgactgatcgatcatcatgctagcta 267 26 0 42.31 62.472 26.79 11.24 41.77 8.472 873 tagctgactgatcgatcatcatgcta 263 26 0 42.31 62.472 33.83 32.61 41.77 8.472 874 actgatcgatcatcatgctagctact 269 26 0 42.31 62.478 26.67 0.00 41.77 8.478 875 gctagtgatgcatgctagtagtgatg 25 26 0 46.15 62.484 24.96 9.07 0.00 8.484 876 ctagctagctactgatcgatgctaca 301 26 0 46.15 62.503 14.00 5.87 0.00 8.503 877 gactgatcgatcgatgctagctagta 116 26 0 46.15 62.543 22.18 18.77 35.21 8.543 878 actgatcgatcgatgctagctagtag 117 26 0 46.15 62.548 23.29 12.42 35.21 8.548 879 ctagctgatcatcatctactatcatc 216 26 0 38.46 57.395 0.00 0.00 0.00 8.605 880 ctgatcatcatcgatgctagctagtag 517 27 0 44.44 61.665 10.30 1.36 40.32 8.665 881 ctactagctagctgatcatcatcgatg 506 27 0 44.44 61.665 20.23 20.23 41.48 8.665 882 ctgatcgatcatcatgctagctactag 270 27 0 44.44 61.665 26.67 8.14 41.77 8.665 883 tagctgatcatcatctactatcatcat 217 27 0 33.33 58.315 0.00 0.00 0.00 8.685 884 tgatgcatgctagtagtgatgtatacg 30 27 0 40.74 61.778 9.81 0.00 0.00 8.778 885 gatgcatgctagtagtgatgtatacgt 31 27 0 40.74 61.779 7.40 0.00 0.00 8.779 886 gctactagctagctgatcatcatcga 505 26 0 46.15 62.779 14.37 0.00 44.92 8.779 887 agctgatcatcgatgctactagctag 194 26 0 46.15 62.784 24.59 20.04 45.92 8.784 888 ctagctgatcatcgatgctactagct 192 26 0 46.15 62.784 27.00 27.00 45.92 8.784 889 agctagctgatcatcgatgctactag 190 26 0 46.15 62.784 20.13 20.13 0.00 8.784 890 ctagctagctgatcatcgatgctact 188 26 0 46.15 62.784 17.55 15.53 0.00 8.784 891 atttagctagctgactgatcgatcatc 256 27 0 40.74 61.785 23.93 21.92 36.62 8.785 892 tctactatcatcatcatctactagct 230 26 0 34.62 57.155 0.00 0.00 0.00 8.845 893 tgctagtgatgcatgctagtagtgat 24 26 0 42.31 62.874 24.96 8.51 35.89 8.874 894 ctgatcatcatctactatcatcatca 220 26 0 34.62 57.026 0.00 0.00 0.00 8.974 895 agctagctactgatcgatgctacatc 303 26 0 46.15 62.999 17.56 7.89 37.97 8.999 896 tagtagtgatgtatacgtagctagtag 40 27 0 37.04 57.869 16.78 1.30 0.00 9.131 897 ctagtagtgatgtatacgtagctagta 39 27 0 37.04 57.869 16.85 15.97 0.00 9.131 898 actgatcgatcatcatgctagctacta 269 27 0 40.74 62.236 26.67 1.37 41.77 9.236 899 gcatgctagtagtgatgtatacgtagc 34 27 0 44.44 62.283 11.60 4.93 0.00 9.283 900 gctatttagctagctgactgatcgatc 253 27 0 44.44 62.291 19.32 19.32 46.11 9.291 901 atctactatcatcatcatctactagct 229 27 0 33.33 57.592 0.00 0.00 0.00 9.408 902 catctactatcatcatcatctactagc 228 27 0 37.04 57.549 0.00 0.00 0.00 9.451 903 tgatcatcatctactatcatcatcatc 221 27 0 33.33 57.467 0.00 0.00 0.00 9.533 904 tagctgatcatcgatgctactagctag 193 27 0 44.44 62.534 27.70 20.04 45.92 9.534 905 ctagctgatcatcgatgctactagcta 192 27 0 44.44 62.534 27.76 26.73 45.92 9.534 906 tagctagctgatcatcgatgctactag 189 27 0 44.44 62.534 20.13 20.13 0.00 9.534 907 ctagctagctgatcatcgatgctacta 188 27 0 44.44 62.534 17.74 15.95 0.00 9.534 908 ctgatcatcatctactatcatcatcat 220 27 0 33.33 57.462 0.00 0.00 0.00 9.538 909 gctagctagctactatcatcgatcgat 426 27 0 44.44 62.627 34.38 25.37 46.11 9.627 910 ttagctagctgactgatcgatcatcat 258 27 0 40.74 62.665 28.29 20.43 41.77 9.665 911 tagctagctactgatcgatgctacatc 302 27 0 44.44 62.742 27.80 7.89 37.97 9.742 912 ctagctagctactgatcgatgctacat 301 27 0 44.44 62.747 14.00 2.51 0.00 9.747 913 gatcatcatctactatcatcatcatct 222 27 0 33.33 57.242 0.00 0.00 0.00 9.758 914 tttagctagctgactgatcgatcatca 257 27 0 40.74 62.820 26.49 26.49 39.98 9.820 915 tctactatcatcatcatctactagcta 230 27 0 33.33 57.100 0.00 0.00 0.00 9.900 primer3-2.3.6/test/primer1_th_list_last/included_test_w_middle.for0000664000077300007730000010144012231156243024365 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TGTCTTGTGGGTGTGTCTGT 81 20 0 50.00 60.024 0.00 0.00 0.00 0.024 1 TGTGTCTTGTGGGTGTGTCT 79 20 0 50.00 60.024 0.00 0.00 0.00 0.024 2 GTGTCTTGTGGGTGTGTCTG 80 20 0 55.00 59.605 0.00 0.00 0.00 0.395 3 GTCTTGTGGGTGTGTCTGTT 82 20 0 50.00 58.879 0.00 0.00 0.00 1.121 4 TGTCTTGTGGGTGTGTCTGTT 81 21 0 47.62 60.657 0.00 0.00 0.00 1.657 5 GAACATGTGTGGCATTGTGT 21 20 0 45.00 58.108 0.00 0.00 35.88 1.892 6 AGCTTCTTGACTGTGTGTTGT 149 21 0 42.86 58.935 0.00 0.00 0.00 2.065 7 AGCTTCTTGACTGTGTGTTGTG 149 22 0 45.45 60.239 0.00 0.00 0.00 2.239 8 GTCTTGTGGGTGTGTCTGTTA 82 21 0 47.62 58.734 0.00 0.00 0.00 2.266 9 TGTGTCTTGTGGGTGTGTCTG 79 21 0 52.38 61.332 0.00 0.00 0.00 2.332 10 GTGTCTTGTGGGTGTGTCTGT 80 21 0 52.38 61.333 0.00 0.00 0.00 2.333 11 TGTGGGTGTGTCTGTTAAACA 86 21 0 42.86 58.579 0.00 0.00 0.00 2.421 12 TGTCTTGTGGGTGTGTCTGTTA 81 22 0 45.45 60.439 0.00 0.00 0.00 2.439 13 TGTGTCTTGTGGGTGTGTC 79 19 0 52.63 58.440 0.00 0.00 0.00 2.560 14 GTCTTGTGGGTGTGTCTGTTAA 82 22 0 45.45 59.389 0.00 0.00 0.00 2.611 15 TCTTGTGGGTGTGTCTGTTA 83 20 0 45.00 57.250 0.00 0.00 0.00 2.750 16 TTGTGGGTGTGTCTGTTAAACA 85 22 0 40.91 59.248 0.00 0.00 0.00 2.752 17 TGTCTTGTGGGTGTGTCTG 81 19 0 52.63 58.133 0.00 0.00 0.00 2.867 18 GTCTTGTGGGTGTGTCTGT 82 19 0 52.63 58.131 0.00 0.00 0.00 2.869 19 GTGTCTTGTGGGTGTGTCT 80 19 0 52.63 58.131 0.00 0.00 0.00 2.869 20 AGCTTCTTGACTGTGTGTTG 149 20 0 45.00 57.112 0.00 0.00 0.00 2.888 21 TGTAGCTTCTTGACTGTGTGT 146 21 0 42.86 58.106 0.00 0.00 0.00 2.894 22 TGGGTGTGTCTGTTAAACATGT 88 22 0 40.91 59.046 0.00 0.00 0.00 2.954 23 TGTGGGTGTGTCTGTTAAACAT 86 22 0 40.91 59.046 0.00 0.00 0.00 2.954 24 TCTTGTGGGTGTGTCTGTTAA 83 21 0 42.86 58.017 0.00 0.00 0.00 2.983 25 GAACATGTGTGGCATTGTGTA 21 21 0 42.86 57.999 0.00 0.00 35.88 3.001 26 TGGATGTAGCTTCTTGACTGTG 142 22 0 45.45 58.995 0.00 0.00 0.00 3.005 27 GGATGTAGCTTCTTGACTGTGT 143 22 0 45.45 58.995 0.00 0.00 0.00 3.005 28 TCTTGTGGGTGTGTCTGTTAAAC 83 23 0 43.48 59.988 0.00 0.00 0.00 3.012 29 GTCTTGTGGGTGTGTCTGTTAAA 82 23 0 43.48 59.988 0.00 0.00 0.00 3.012 30 GTAGCTTCTTGACTGTGTGTTGT 147 23 0 43.48 60.052 0.00 0.00 0.00 3.052 31 TAGCTTCTTGACTGTGTGTTGTG 148 23 0 43.48 60.052 0.00 0.00 0.00 3.052 32 TGTAGCTTCTTGACTGTGTGTTG 146 23 0 43.48 60.052 0.00 0.00 0.00 3.052 33 TGTGTGTTGTGTATGGATGTAGC 129 23 0 43.48 59.926 0.00 0.00 0.00 3.074 34 TAGCTTCTTGACTGTGTGTTGT 148 22 0 40.91 58.793 0.00 0.00 0.00 3.207 35 TGTAGCTTCTTGACTGTGTGTT 146 22 0 40.91 58.793 0.00 0.00 0.00 3.207 36 GGATGTAGCTTCTTGACTGTGTG 143 23 0 47.83 60.238 0.00 0.00 0.00 3.238 37 CTTGTGGGTGTGTCTGTTAAACA 84 23 0 43.48 60.238 0.00 0.00 0.00 3.238 38 TCTTGTGGGTGTGTCTGTTAAA 83 22 0 40.91 58.714 0.00 0.00 0.00 3.286 39 TGGGTGTGTCTGTTAAACATGTG 88 23 0 43.48 60.301 0.00 0.00 0.00 3.301 40 TGTGGGTGTGTCTGTTAAACATG 86 23 0 43.48 60.301 0.00 0.00 0.00 3.301 41 GGGTGTGTCTGTTAAACATGTGT 89 23 0 43.48 60.301 0.00 0.00 0.00 3.301 42 GTGGGTGTGTCTGTTAAACATGT 87 23 0 43.48 60.301 0.00 0.00 0.00 3.301 43 GGGTGTGTCTGTTAAACATGTG 89 22 0 45.45 58.689 0.00 0.00 0.00 3.311 44 GTGGGTGTGTCTGTTAAACATG 87 22 0 45.45 58.689 0.00 0.00 0.00 3.311 45 GTGTGTTGTGTATGGATGTAGCT 130 23 0 43.48 59.675 0.00 0.00 0.00 3.325 46 TGTGTCTGTTAAACATGTGTGGT 92 23 0 39.13 59.671 0.00 0.00 0.00 3.329 47 TTGTGGGTGTGTCTGTTAAACAT 85 23 0 39.13 59.668 0.00 0.00 0.00 3.332 48 CTTGTGGGTGTGTCTGTTAAAC 84 22 0 45.45 58.625 0.00 0.00 0.00 3.375 49 GATGTAGCTTCTTGACTGTGTGT 144 23 0 43.48 59.615 0.00 0.00 0.00 3.385 50 ACATGTGTGGCATTGTGTATACA 23 23 0 39.13 59.607 7.27 4.43 35.88 3.393 51 TGGATGTAGCTTCTTGACTGT 142 21 0 42.86 57.597 0.00 0.00 0.00 3.403 52 ATGTAGCTTCTTGACTGTGTGT 145 22 0 40.91 58.590 0.00 0.00 0.00 3.410 53 AAACATGTGTGGTATTGTGCA 102 21 0 38.10 57.554 0.00 0.00 0.00 3.446 54 ACATGTGTGTTGTGTATGGATGT 126 23 0 39.13 59.542 0.00 0.00 34.85 3.458 55 TTGTGGGTGTGTCTGTTAAAC 85 21 0 42.86 57.507 0.00 0.00 0.00 3.493 56 GAACATGTGTGGCATTGTGTAT 21 22 0 40.91 58.480 0.00 0.00 35.88 3.520 57 TGTGTGGCATTGTGTATACAC 26 21 0 42.86 57.451 26.66 26.66 46.84 3.549 58 GTAGCTTCTTGACTGTGTGTTG 147 22 0 45.45 58.445 0.00 0.00 0.00 3.555 59 ATGGATGTAGCTTCTTGACTGTG 141 23 0 43.48 59.421 0.00 0.00 0.00 3.579 60 TGTGTTGTGTATGGATGTAGCT 131 22 0 40.91 58.384 0.00 0.00 0.00 3.616 61 TGGATGTAGCTTCTTGACTGTGT 142 23 0 43.48 60.620 0.00 0.00 0.00 3.620 62 AACATGTGTGGTATTGTGCAT 103 21 0 38.10 57.337 0.00 0.00 0.00 3.663 63 AACATGTGTGGCATTGTGTAT 22 21 0 38.10 57.337 0.00 0.00 35.88 3.663 64 TCTTGTGGGTGTGTCTGTT 83 19 0 47.37 57.331 0.00 0.00 0.00 3.669 65 GTGTGTCTGTTAAACATGTGTGG 91 23 0 43.48 59.315 0.00 0.00 0.00 3.685 66 GGTGTGTCTGTTAAACATGTGTG 90 23 0 43.48 59.315 0.00 0.00 0.00 3.685 67 GTGTGTTGTGTATGGATGTAGC 130 22 0 45.45 58.308 0.00 0.00 0.00 3.692 68 TGGGTGTGTCTGTTAAACATG 88 21 0 42.86 57.293 0.00 0.00 0.00 3.707 69 GGGTGTGTCTGTTAAACATGT 89 21 0 42.86 57.292 0.00 0.00 0.00 3.708 70 GTGGGTGTGTCTGTTAAACAT 87 21 0 42.86 57.292 0.00 0.00 0.00 3.708 71 GGATGTAGCTTCTTGACTGTG 143 21 0 47.62 57.263 0.00 0.00 0.00 3.737 72 CATGTGTGGCATTGTGTATACAC 24 23 0 43.48 59.252 26.66 26.66 41.05 3.748 73 ATGTAGCTTCTTGACTGTGTGTT 145 23 0 39.13 59.228 0.00 0.00 0.00 3.772 74 CTTGTGGGTGTGTCTGTTAAA 84 21 0 42.86 57.225 0.00 0.00 0.00 3.775 75 GTGTCTTGTGGGTGTGTCTGTT 80 22 0 50.00 61.870 0.00 0.00 0.00 3.870 76 ATGGATGTAGCTTCTTGACTGT 141 22 0 40.91 58.107 0.00 0.00 0.00 3.893 77 AAACATGTGTGGTATTGTGCAT 102 22 0 36.36 58.061 0.00 0.00 0.00 3.939 78 TAGCTTCTTGACTGTGTGTTG 148 21 0 42.86 57.047 0.00 0.00 0.00 3.953 79 GTAGCTTCTTGACTGTGTGTT 147 21 0 42.86 57.045 0.00 0.00 0.00 3.955 80 TGTGTTGTGTATGGATGTAGCTT 131 23 0 39.13 59.034 0.00 0.00 0.00 3.966 81 TGTGTCTGTTAAACATGTGTGG 92 22 0 40.91 58.026 0.00 0.00 0.00 3.974 82 GTGTCTGTTAAACATGTGTGGT 93 22 0 40.91 58.025 0.00 0.00 0.00 3.975 83 GGTGTGTCTGTTAAACATGTGT 90 22 0 40.91 58.025 0.00 0.00 0.00 3.975 84 TGTCTTGTGGGTGTGTCTGTTAA 81 23 0 43.48 61.000 0.00 0.00 0.00 4.000 85 TGTGTTGTGTATGGATGTAGCTTC 131 24 0 41.67 59.997 0.00 0.00 0.00 4.003 86 GATGTAGCTTCTTGACTGTGTG 144 22 0 45.45 57.988 0.00 0.00 0.00 4.012 87 CATGTGTGGCATTGTGTATACA 24 22 0 40.91 57.958 7.27 4.43 35.88 4.042 88 ATGTGTGGCATTGTGTATACAC 25 22 0 40.91 57.956 26.66 26.66 46.84 4.044 89 ACATGTGTGGCATTGTGTATAC 23 22 0 40.91 57.956 0.00 0.00 35.88 4.044 90 CATGTGTGTTGTGTATGGATGT 127 22 0 40.91 57.887 0.00 0.00 0.00 4.113 91 ACATGTGTGTTGTGTATGGATG 126 22 0 40.91 57.887 0.00 0.00 34.85 4.113 92 AACATGTGTGGCATTGTGTATACA 22 24 0 37.50 60.177 7.27 4.43 35.88 4.177 93 TGTGCATATACATGTGTGTTGTGT 117 24 0 37.50 60.177 3.23 0.00 40.30 4.177 94 GATGTAGCTTCTTGACTGTGTGTT 144 24 0 41.67 60.177 0.00 0.00 0.00 4.177 95 GTGTGTTGTGTATGGATGTAGCTT 130 24 0 41.67 60.237 0.00 0.00 0.00 4.237 96 GTGTTGTGTATGGATGTAGCTTCT 132 24 0 41.67 59.756 0.00 0.00 0.00 4.244 97 ATGTGTGTTGTGTATGGATGTAGC 128 24 0 41.67 60.297 0.00 0.00 0.00 4.297 98 TGTGCATATACATGTGTGTTGTG 117 23 0 39.13 58.624 3.23 0.00 40.30 4.376 99 GTGCATATACATGTGTGTTGTGT 118 23 0 39.13 58.623 3.23 0.00 40.30 4.377 100 AACATGTGTGGCATTGTGTATAC 22 23 0 39.13 58.615 0.00 0.00 35.88 4.385 101 GAACATGTGTGGCATTGTGTATAC 21 24 0 41.67 59.586 0.00 0.00 35.88 4.414 102 ATGTAGCTTCTTGACTGTGTGTTG 145 24 0 41.67 60.416 0.00 0.00 0.00 4.416 103 TGTTGTGTATGGATGTAGCTTCT 133 23 0 39.13 58.521 0.00 0.00 0.00 4.479 104 TGTGTATGGATGTAGCTTCTTGAC 136 24 0 41.67 59.515 0.00 0.00 0.00 4.485 105 TGTGTCTGTTAAACATGTGTGGTA 92 24 0 37.50 59.511 0.00 0.00 0.00 4.489 106 TAAACATGTGTGGTATTGTGCA 101 22 0 36.36 57.471 0.00 0.00 0.00 4.529 107 GTGTTGTGTATGGATGTAGCTTC 132 23 0 43.48 58.444 0.00 0.00 0.00 4.556 108 GTTAAACATGTGTGGTATTGTGCA 99 24 0 37.50 59.401 0.00 0.00 0.00 4.599 109 TGTTAAACATGTGTGGTATTGTGC 98 24 0 37.50 59.401 0.00 0.00 0.00 4.599 110 CTTGTGGGTGTGTCTGTTAAACAT 84 24 0 41.67 60.599 0.00 0.00 0.00 4.599 111 ACATGTGTGTTGTGTATGGATGTA 126 24 0 37.50 59.387 0.00 0.00 34.85 4.613 112 TACATGTGTGTTGTGTATGGATGT 125 24 0 37.50 59.387 9.61 0.00 40.30 4.613 113 GTGTCTTGTGGGTGTGTCTGTTA 80 23 0 47.83 61.614 0.00 0.00 0.00 4.614 114 GAACATGTGTGGCATTGTGTATA 21 23 0 39.13 58.364 0.00 0.00 35.88 4.636 115 GTGTTGTGTATGGATGTAGCTT 132 22 0 40.91 57.359 0.00 0.00 0.00 4.641 116 TGCATATACATGTGTGTTGTGT 119 22 0 36.36 57.281 3.23 0.00 40.30 4.719 117 TGTGCATATACATGTGTGTTGT 117 22 0 36.36 57.281 3.23 0.00 40.30 4.719 118 TATGGATGTAGCTTCTTGACTGTG 140 24 0 41.67 59.271 0.00 0.00 0.00 4.729 119 TGTATGGATGTAGCTTCTTGACTG 138 24 0 41.67 59.271 0.00 0.00 0.00 4.729 120 GTATGGATGTAGCTTCTTGACTGT 139 24 0 41.67 59.271 0.00 0.00 0.00 4.729 121 GTGTATGGATGTAGCTTCTTGACT 137 24 0 41.67 59.271 0.00 0.00 0.00 4.729 122 TGTGTATGGATGTAGCTTCTTGA 136 23 0 39.13 58.265 0.00 0.00 0.00 4.735 123 AACATGTGTGGTATTGTGCATA 103 22 0 36.36 57.263 0.00 0.00 0.00 4.737 124 AACATGTGTGGCATTGTGTATA 22 22 0 36.36 57.263 0.00 0.00 35.88 4.737 125 TTGTGCATATACATGTGTGTTGTG 116 24 0 37.50 59.225 3.23 0.00 40.30 4.775 126 ACATGTGTGGCATTGTGTATACAC 23 24 0 41.67 60.775 26.66 26.66 41.05 4.775 127 AGCTTCTTGACTGTGTGTTGTGT 149 23 0 43.48 61.808 0.00 0.00 0.00 4.808 128 GTGTGTCTGTTAAACATGTGTGGT 91 24 0 41.67 60.834 0.00 0.00 0.00 4.834 129 TTGTGGGTGTGTCTGTTAAACATG 85 24 0 41.67 60.837 0.00 0.00 0.00 4.837 130 TTAAACATGTGTGGTATTGTGCA 100 23 0 34.78 58.157 0.00 0.00 0.00 4.843 131 TGTTGTGTATGGATGTAGCTTCTT 133 24 0 37.50 59.140 0.00 0.00 0.00 4.860 132 TGTGTCTTGTGGGTGTGT 79 18 0 50.00 57.105 0.00 0.00 0.00 4.895 133 TGTTGTGTATGGATGTAGCTTC 133 22 0 40.91 57.096 0.00 0.00 0.00 4.904 134 ACATGTGTGGTATTGTGCATAT 104 22 0 36.36 57.054 0.00 0.00 0.00 4.946 135 TGTGTCTTGTGGGTGTGTCTGT 79 22 0 50.00 62.948 0.00 0.00 0.00 4.948 136 GTGTGTCTGTTAAACATGTGTG 91 22 0 40.91 57.042 0.00 0.00 0.00 4.958 137 TGTGTGTTGTGTATGGATGTAG 129 22 0 40.91 57.033 0.00 0.00 0.00 4.967 138 ATGGATGTAGCTTCTTGACTGTGT 141 24 0 41.67 60.971 0.00 0.00 0.00 4.971 139 TATGGATGTAGCTTCTTGACTGT 140 23 0 39.13 58.004 0.00 0.00 0.00 4.996 140 TGTATGGATGTAGCTTCTTGACT 138 23 0 39.13 58.004 0.00 0.00 0.00 4.996 141 TGTGCATATACATGTGTGTTGTGTA 117 25 0 36.00 60.005 3.23 0.00 40.30 5.005 142 TTGTGCATATACATGTGTGTTGT 116 23 0 34.78 57.973 3.23 0.00 40.30 5.027 143 AAACATGTGTGGTATTGTGCATA 102 23 0 34.78 57.961 0.00 0.00 0.00 5.039 144 TAAACATGTGTGGTATTGTGCAT 101 23 0 34.78 57.961 0.00 0.00 0.00 5.039 145 GTGTATGGATGTAGCTTCTTGAC 137 23 0 43.48 57.941 0.00 0.00 0.00 5.059 146 TTGTGTATGGATGTAGCTTCTTGAC 135 25 0 40.00 60.063 0.00 0.00 0.00 5.063 147 GTTGTGTATGGATGTAGCTTCTTGA 134 25 0 40.00 60.063 0.00 0.00 0.00 5.063 148 GTGTCTGTTAAACATGTGTGGTA 93 23 0 39.13 57.927 0.00 0.00 0.00 5.073 149 TTGTGTATGGATGTAGCTTCTTGA 135 24 0 37.50 58.896 0.00 0.00 0.00 5.104 150 TGTGTCTGTTAAACATGTGTGGTAT 92 25 0 36.00 59.887 0.00 0.00 0.00 5.113 151 GTCTTGTGGGTGTGTCTGTTAAAC 82 24 0 45.83 61.126 0.00 0.00 0.00 5.126 152 GTTAAACATGTGTGGTATTGTGC 99 23 0 39.13 57.839 0.00 0.00 0.00 5.161 153 TGGTATTGTGCATATACATGTGTGT 111 25 0 36.00 59.828 18.75 0.00 42.66 5.172 154 TGTGGTATTGTGCATATACATGTGT 109 25 0 36.00 59.828 8.22 0.00 34.94 5.172 155 TGTGTGGTATTGTGCATATACATGT 107 25 0 36.00 59.828 8.22 3.20 34.94 5.172 156 ACATGTGTGGTATTGTGCATATACA 104 25 0 36.00 59.828 18.75 7.54 0.00 5.172 157 GTAGCTTCTTGACTGTGTGTTGTG 147 24 0 45.83 61.177 0.00 0.00 0.00 5.177 158 CATGTGTGTTGTGTATGGATGTAG 127 24 0 41.67 58.810 0.00 0.00 0.00 5.190 159 GTTGTGTATGGATGTAGCTTCTTG 134 24 0 41.67 58.809 0.00 0.00 0.00 5.191 160 CATGTGTGTTGTGTATGGATGTA 127 23 0 39.13 57.795 0.00 0.00 0.00 5.205 161 TACATGTGTGTTGTGTATGGATG 125 23 0 39.13 57.795 9.61 0.00 40.30 5.205 162 TGTGTGTTGTGTATGGATGTAGCT 129 24 0 41.67 61.209 0.00 0.00 0.00 5.209 163 GTTAAACATGTGTGGTATTGTGCAT 99 25 0 36.00 59.776 0.00 0.00 0.00 5.224 164 ATACATGTGTGTTGTGTATGGATGT 124 25 0 36.00 59.769 9.61 0.00 38.35 5.231 165 AACATGTGTGGTATTGTGCATAT 103 23 0 34.78 57.763 0.00 0.00 0.00 5.237 166 CTGTTAAACATGTGTGGTATTGTGC 97 25 0 40.00 60.292 0.00 0.00 0.00 5.292 167 TGTTGTGTATGGATGTAGCTTCTTG 133 25 0 40.00 60.294 0.00 0.00 0.00 5.294 168 ACATGTGTGTTGTGTATGGATGTAG 126 25 0 40.00 60.294 0.00 0.00 34.85 5.294 169 GTGTTGTGTATGGATGTAGCTTCTT 132 25 0 40.00 60.294 0.00 0.00 0.00 5.294 170 GTATGGATGTAGCTTCTTGACTG 139 23 0 43.48 57.686 0.00 0.00 0.00 5.314 171 ATTGTGCATATACATGTGTGTTGTG 115 25 0 36.00 59.605 3.23 0.00 40.30 5.395 172 TTAAACATGTGTGGTATTGTGCAT 100 24 0 33.33 58.600 0.00 0.00 0.00 5.400 173 GTATGGATGTAGCTTCTTGACTGTG 139 25 0 44.00 60.408 0.00 0.00 0.00 5.408 174 GTGTATGGATGTAGCTTCTTGACTG 137 25 0 44.00 60.408 0.00 0.00 0.00 5.408 175 GGTGTGTCTGTTAAACATGTGTGG 90 24 0 45.83 61.422 0.00 0.00 0.00 5.422 176 GGGTGTGTCTGTTAAACATGTGTG 89 24 0 45.83 61.422 0.00 0.00 0.00 5.422 177 GTGGGTGTGTCTGTTAAACATGTG 87 24 0 45.83 61.422 0.00 0.00 0.00 5.422 178 TTGTGTATGGATGTAGCTTCTTG 135 23 0 39.13 57.536 0.00 0.00 0.00 5.464 179 ATGTGTGTTGTGTATGGATGTAG 128 23 0 39.13 57.536 0.00 0.00 0.00 5.464 180 GTTGTGTATGGATGTAGCTTCTT 134 23 0 39.13 57.535 0.00 0.00 0.00 5.465 181 GTGCATATACATGTGTGTTGTGTA 118 24 0 37.50 58.507 3.23 0.00 40.30 5.493 182 GCATATACATGTGTGTTGTGTATGG 120 25 0 40.00 59.494 17.56 6.84 44.43 5.506 183 GTGGTATTGTGCATATACATGTGTG 110 25 0 40.00 59.494 8.22 4.60 34.94 5.506 184 GTGTGGTATTGTGCATATACATGTG 108 25 0 40.00 59.494 9.61 0.00 34.94 5.506 185 TCTTGTGGGTGTGTCTGTTAAACA 83 24 0 41.67 61.514 0.00 0.00 0.00 5.514 186 TGTCTTGTGGGTGTGTCTGTTAAA 81 24 0 41.67 61.514 0.00 0.00 0.00 5.514 187 TAGCTTCTTGACTGTGTGTTGTGT 148 24 0 41.67 61.564 0.00 0.00 0.00 5.564 188 TGTAGCTTCTTGACTGTGTGTTGT 146 24 0 41.67 61.564 0.00 0.00 0.00 5.564 189 ATTGTGCATATACATGTGTGTTGT 115 24 0 33.33 58.422 3.23 0.00 40.30 5.578 190 AAACATGTGTGGTATTGTGCATAT 102 24 0 33.33 58.412 0.00 0.00 0.00 5.588 191 GTGTCTGTTAAACATGTGTGGTAT 93 24 0 37.50 58.375 0.00 0.00 0.00 5.625 192 GTGTGTCTGTTAAACATGTGTGGTA 91 25 0 40.00 60.639 0.00 0.00 0.00 5.639 193 TGGTATTGTGCATATACATGTGTG 111 24 0 37.50 58.313 18.75 4.60 34.94 5.687 194 TGTGGTATTGTGCATATACATGTG 109 24 0 37.50 58.313 9.61 0.00 34.94 5.687 195 TGTGTGGTATTGTGCATATACATG 107 24 0 37.50 58.313 8.22 0.00 35.35 5.687 196 CATGTGTGGTATTGTGCATATACA 105 24 0 37.50 58.313 18.75 7.54 0.00 5.687 197 GGTATTGTGCATATACATGTGTGT 112 24 0 37.50 58.312 18.75 0.00 42.66 5.688 198 GTGGTATTGTGCATATACATGTGT 110 24 0 37.50 58.312 8.22 0.00 34.94 5.688 199 GTGTGGTATTGTGCATATACATGT 108 24 0 37.50 58.312 8.22 3.20 34.94 5.688 200 ACATGTGTGGTATTGTGCATATAC 104 24 0 37.50 58.312 12.13 12.13 0.00 5.688 201 TTGTGCATATACATGTGTGTTGTGT 116 25 0 36.00 60.700 3.23 0.00 40.30 5.700 202 TGGATGTAGCTTCTTGACTGTGTG 142 24 0 45.83 61.738 0.00 0.00 0.00 5.738 203 GGATGTAGCTTCTTGACTGTGTGT 143 24 0 45.83 61.739 0.00 0.00 0.00 5.739 204 ATACATGTGTGTTGTGTATGGATG 124 24 0 37.50 58.249 9.61 0.00 38.35 5.751 205 TACATGTGTGTTGTGTATGGATGTA 125 25 0 36.00 59.243 9.61 0.00 40.30 5.757 206 TATGGATGTAGCTTCTTGACTGTGT 140 25 0 40.00 60.765 0.00 0.00 0.00 5.765 207 TGTATGGATGTAGCTTCTTGACTGT 138 25 0 40.00 60.765 0.00 0.00 0.00 5.765 208 TGTGTATGGATGTAGCTTCTTGACT 136 25 0 40.00 60.765 0.00 0.00 0.00 5.765 209 TGCATATACATGTGTGTTGTGTA 119 23 0 34.78 57.213 3.23 0.00 40.30 5.787 210 TGGGTGTGTCTGTTAAACATGTGT 88 24 0 41.67 61.817 0.00 0.00 0.00 5.817 211 TGTGGGTGTGTCTGTTAAACATGT 86 24 0 41.67 61.817 0.00 0.00 0.00 5.817 212 TGTTAAACATGTGTGGTATTGTGCA 98 25 0 36.00 60.875 0.00 0.00 0.00 5.875 213 TGTCTGTTAAACATGTGTGGTAT 94 23 0 34.78 57.063 0.00 0.00 0.00 5.937 214 GGTATTGTGCATATACATGTGTGTTG 112 26 0 38.46 60.015 18.75 0.00 40.30 6.015 215 GTGCATATACATGTGTGTTGTGTATG 118 26 0 38.46 60.016 13.84 13.84 45.64 6.016 216 TCTGTTAAACATGTGTGGTATTGTG 96 25 0 36.00 58.967 0.00 0.00 0.00 6.033 217 TGTCTGTTAAACATGTGTGGTATTG 94 25 0 36.00 58.967 0.00 0.00 0.00 6.033 218 GTCTGTTAAACATGTGTGGTATTGT 95 25 0 36.00 58.967 0.00 0.00 0.00 6.033 219 GTGTCTGTTAAACATGTGTGGTATT 93 25 0 36.00 58.967 0.00 0.00 0.00 6.033 220 GAACATGTGTGGCATTGTGTATACA 21 25 0 40.00 61.041 7.27 4.43 35.88 6.041 221 GTCTGTTAAACATGTGTGGTATTGTG 95 26 0 38.46 60.070 0.00 0.00 0.00 6.070 222 GTGTCTGTTAAACATGTGTGGTATTG 93 26 0 38.46 60.070 0.00 0.00 0.00 6.070 223 TGCATATACATGTGTGTTGTGTATG 119 25 0 36.00 58.914 13.84 13.84 45.64 6.086 224 GTGCATATACATGTGTGTTGTGTAT 118 25 0 36.00 58.914 4.13 3.79 41.36 6.086 225 GTGTGTTGTGTATGGATGTAGCTTC 130 25 0 44.00 61.090 0.00 0.00 0.00 6.090 226 GGTATTGTGCATATACATGTGTGTT 112 25 0 36.00 58.907 18.75 0.00 40.30 6.093 227 AACATGTGTGGTATTGTGCATATAC 103 25 0 36.00 58.907 12.13 12.13 0.00 6.093 228 GTGTCTTGTGGGTGTGTCTGTTAA 80 24 0 45.83 62.097 0.00 0.00 0.00 6.097 229 TACATGTGTGTTGTGTATGGATGTAG 125 26 0 38.46 60.124 9.61 0.00 40.30 6.124 230 TAAACATGTGTGGTATTGTGCATA 101 24 0 33.33 57.868 0.00 0.00 0.00 6.132 231 CATGTGTGGTATTGTGCATATACATG 105 26 0 38.46 59.850 13.99 9.60 35.35 6.150 232 ATGTGTGGTATTGTGCATATACATGT 106 26 0 34.62 60.180 8.22 3.20 34.94 6.180 233 ACATGTGTGGTATTGTGCATATACAT 104 26 0 34.62 60.180 8.22 0.00 0.00 6.180 234 TGTGTTGTGTATGGATGTAGCTTCT 131 25 0 40.00 61.231 0.00 0.00 0.00 6.231 235 GATGTAGCTTCTTGACTGTGTGTTG 144 25 0 44.00 61.257 0.00 0.00 0.00 6.257 236 TCTGTTAAACATGTGTGGTATTGT 96 24 0 33.33 57.742 0.00 0.00 0.00 6.258 237 TGTCTGTTAAACATGTGTGGTATT 94 24 0 33.33 57.742 0.00 0.00 0.00 6.258 238 ATGTGTGGTATTGTGCATATACATG 106 25 0 36.00 58.730 8.22 0.00 35.35 6.270 239 CATGTGTGGTATTGTGCATATACAT 105 25 0 36.00 58.730 8.22 0.00 0.00 6.270 240 AACATGTGTGGCATTGTGTATACAC 22 25 0 40.00 61.271 26.66 26.66 41.05 6.271 241 TGCATATACATGTGTGTTGTGTAT 119 24 0 33.33 57.692 3.79 3.79 41.36 6.308 242 CTGTTAAACATGTGTGGTATTGTG 97 24 0 37.50 57.686 0.00 0.00 0.00 6.314 243 AACATGTGTGGTATTGTGCATATA 103 24 0 33.33 57.678 0.00 0.00 0.00 6.322 244 TGTGCATATACATGTGTGTTGTGTAT 117 26 0 34.62 60.348 4.13 3.79 41.36 6.348 245 TGGTATTGTGCATATACATGTGTGTT 111 26 0 34.62 60.349 18.75 0.00 40.30 6.349 246 AACATGTGTGGTATTGTGCATATACA 103 26 0 34.62 60.349 18.75 7.54 0.00 6.349 247 CATGTGTGTTGTGTATGGATGTAGC 127 25 0 44.00 61.374 0.00 0.00 0.00 6.374 248 GTTAAACATGTGTGGTATTGTGCATA 99 26 0 34.62 59.626 0.00 0.00 0.00 6.374 249 ATACATGTGTGTTGTGTATGGATGTA 124 26 0 34.62 59.616 9.61 0.00 38.35 6.384 250 TATACATGTGTGTTGTGTATGGATGT 123 26 0 34.62 59.616 7.92 0.00 39.64 6.384 251 TGTCTGTTAAACATGTGTGGTATTGT 94 26 0 34.62 60.405 0.00 0.00 0.00 6.405 252 TGTGTCTGTTAAACATGTGTGGTATT 92 26 0 34.62 60.405 0.00 0.00 0.00 6.405 253 TTAAACATGTGTGGTATTGTGCATA 100 25 0 32.00 58.487 0.00 0.00 0.00 6.513 254 ATGTGTGGTATTGTGCATATACAT 106 24 0 33.33 57.486 8.22 0.00 0.00 6.514 255 TTGTGCATATACATGTGTGTTGTGTA 116 26 0 34.62 60.515 3.23 0.00 40.30 6.515 256 ATGTGTGTTGTGTATGGATGTAGCT 128 25 0 40.00 61.523 0.00 0.00 0.00 6.523 257 TATTGTGCATATACATGTGTGTTGTG 114 26 0 34.62 59.461 3.23 0.00 40.30 6.539 258 GTATTGTGCATATACATGTGTGTTGT 113 26 0 34.62 59.461 14.51 0.00 40.30 6.539 259 AAACATGTGTGGTATTGTGCATATAC 102 26 0 34.62 59.457 12.13 12.13 0.00 6.543 260 GTCTGTTAAACATGTGTGGTATTG 95 24 0 37.50 57.446 0.00 0.00 0.00 6.554 261 GCATATACATGTGTGTTGTGTATG 120 24 0 37.50 57.398 13.84 13.84 45.64 6.602 262 TGTGTTGTGTATAAATGTTGTGTCT 50 25 0 32.00 58.377 0.00 0.00 0.00 6.623 263 CTTGTGGGTGTGTCTGTTAAACATG 84 25 0 44.00 61.663 0.00 0.00 0.00 6.663 264 GCATATACATGTGTGTTGTGTATGGA 120 26 0 38.46 60.680 17.56 0.00 44.43 6.680 265 TATTGTGCATATACATGTGTGTTGT 114 25 0 32.00 58.316 3.23 0.00 40.30 6.684 266 AAACATGTGTGGTATTGTGCATATA 102 25 0 32.00 58.306 0.00 0.00 0.00 6.694 267 TAAACATGTGTGGTATTGTGCATAT 101 25 0 32.00 58.306 0.00 0.00 0.00 6.694 268 TGTGTGTTGTGTATGGATGTAGCTT 129 25 0 40.00 61.694 0.00 0.00 0.00 6.694 269 TCTTGTGGGTGTGTCTGTTAAACAT 83 25 0 40.00 61.817 0.00 0.00 0.00 6.817 270 AGCTTCTTGACTGTGTGTTGTGTG 149 24 0 45.83 62.850 0.00 0.00 0.00 6.850 271 TATACATGTGTGTTGTGTATGGATG 123 25 0 36.00 58.150 7.92 0.00 39.64 6.850 272 CATATACATGTGTGTTGTGTATGGA 121 25 0 36.00 58.150 11.52 0.00 42.41 6.850 273 ATGTAGCTTCTTGACTGTGTGTTGT 145 25 0 40.00 61.863 0.00 0.00 0.00 6.863 274 TGCATATACATGTGTGTTGTGTATGG 119 26 0 38.46 60.902 17.56 6.84 44.43 6.902 275 TGTGGTATTGTGCATATACATGTGTG 109 26 0 38.46 60.902 8.22 4.60 34.94 6.902 276 TGTGTGGTATTGTGCATATACATGTG 107 26 0 38.46 60.902 9.61 0.00 34.94 6.902 277 GTGGTATTGTGCATATACATGTGTGT 110 26 0 38.46 60.902 9.41 0.00 42.66 6.902 278 GTGTGGTATTGTGCATATACATGTGT 108 26 0 38.46 60.902 8.22 0.00 34.94 6.902 279 TGTGTTGTGTATAAATGTTGTGTC 50 24 0 33.33 57.075 0.00 0.00 0.00 6.925 280 GTGTGTCTGTTAAACATGTGTGGTAT 91 26 0 38.46 60.957 0.00 0.00 0.00 6.957 281 GTATTGTGCATATACATGTGTGTTG 113 25 0 36.00 58.019 14.51 0.00 40.30 6.981 282 ATTGTGCATATACATGTGTGTTGTGT 115 26 0 34.62 61.018 3.23 0.00 40.30 7.018 283 ATGGATGTAGCTTCTTGACTGTGTG 141 25 0 44.00 62.029 0.00 0.00 0.00 7.029 284 GTTAAACATGTGTGGTATTGTGCATAT 99 27 0 33.33 59.967 0.00 0.00 0.00 7.033 285 ATATACATGTGTGTTGTGTATGGATGT 122 27 0 33.33 59.963 7.92 0.00 39.64 7.037 286 TGTGTTGTGTATAAATGTTGTGTCTT 50 26 0 30.77 58.950 0.00 0.00 0.00 7.050 287 TTAAACATGTGTGGTATTGTGCATAT 100 26 0 30.77 58.886 0.00 0.00 0.00 7.114 288 GTTGTGTATGGATGTAGCTTCTTGAC 134 26 0 42.31 61.115 0.00 0.00 0.00 7.115 289 TGTTAAACATGTGTGGTATTGTGCAT 98 26 0 34.62 61.187 0.00 0.00 0.00 7.187 290 GTGTTGTGTATAAATGTTGTGTCTTCA 51 27 0 33.33 59.809 0.00 0.00 0.00 7.191 291 TGTGTTGTGTATAAATGTTGTGTCTTC 50 27 0 33.33 59.809 0.00 0.00 0.00 7.191 292 GGATGTAGCTTCTTGACTGTGTGTT 143 25 0 44.00 62.198 0.00 0.00 0.00 7.198 293 ATGTGTTGTGTATAAATGTTGTGTCT 49 26 0 30.77 58.778 0.00 0.00 0.00 7.222 294 TTGTGTATGGATGTAGCTTCTTGACT 135 26 0 38.46 61.252 0.00 0.00 0.00 7.252 295 TGTTGTGTATAAATGTTGTGTCTTCA 52 26 0 30.77 58.726 0.00 0.00 0.00 7.274 296 TTGTGGGTGTGTCTGTTAAACATGT 85 25 0 40.00 62.278 0.00 0.00 0.00 7.278 297 GTGTTGTGTATGGATGTAGCTTCTTG 132 26 0 42.31 61.334 0.00 0.00 0.00 7.334 298 CATATACATGTGTGTTGTGTATGGATG 121 27 0 37.04 59.649 11.52 3.24 42.41 7.351 299 ATATACATGTGTGTTGTGTATGGATG 122 26 0 34.62 58.558 7.92 0.00 39.64 7.442 300 CATATACATGTGTGTTGTGTATGGAT 121 26 0 34.62 58.558 11.52 0.00 42.41 7.442 301 ATACATGTGTGTTGTGTATGGATGTAG 124 27 0 37.04 60.450 9.61 0.00 38.35 7.450 302 TCTGTTAAACATGTGTGGTATTGTGC 96 26 0 38.46 61.452 0.00 0.00 0.00 7.452 303 ATGTGTTGTGTATAAATGTTGTGTC 49 25 0 32.00 57.534 0.00 0.00 0.00 7.466 304 TGTTGTGTATGGATGTAGCTTCTTGA 133 26 0 38.46 61.474 0.00 0.00 0.00 7.474 305 GTATTGTGCATATACATGTGTGTTGTG 113 27 0 37.04 60.497 14.51 0.00 40.30 7.497 306 GTGTTGTGTATAAATGTTGTGTCTT 51 25 0 32.00 57.477 0.00 0.00 0.00 7.523 307 TATACATGTGTGTTGTGTATGGATGTA 123 27 0 33.33 59.474 7.92 0.00 39.64 7.526 308 GTCTTGTGGGTGTGTCTGTTAAACA 82 25 0 44.00 62.541 0.00 0.00 0.00 7.541 309 TGTCTTGTGGGTGTGTCTGTTAAAC 81 25 0 44.00 62.541 0.00 0.00 0.00 7.541 310 GTGTCTTGTGGGTGTGTCTGTTAAA 80 25 0 44.00 62.541 0.00 0.00 0.00 7.541 311 GTGTTGTGTATAAATGTTGTGTCTTC 51 26 0 34.62 58.430 0.00 0.00 0.00 7.570 312 TAGCTTCTTGACTGTGTGTTGTGTG 148 25 0 44.00 62.579 0.00 0.00 0.00 7.579 313 TGTAGCTTCTTGACTGTGTGTTGTG 146 25 0 44.00 62.579 0.00 0.00 0.00 7.579 314 GTAGCTTCTTGACTGTGTGTTGTGT 147 25 0 44.00 62.581 0.00 0.00 0.00 7.581 315 ATATACATGTGTGTTGTGTATGGAT 122 25 0 32.00 57.351 7.92 0.00 39.64 7.649 316 AACATGTGTGGTATTGTGCATATACAT 103 27 0 33.33 60.670 8.22 0.00 0.00 7.670 317 CTGTTAAACATGTGTGGTATTGTGCA 97 26 0 38.46 61.672 0.00 0.00 0.00 7.672 318 TAAACATGTGTGGTATTGTGCATATAC 101 27 0 33.33 59.323 12.13 12.13 0.00 7.677 319 ATGTGTTGTGTATAAATGTTGTGTCTT 49 27 0 29.63 59.317 0.00 0.00 0.00 7.683 320 TGTGTTGTGTATGGATGTAGCTTCTT 131 26 0 38.46 61.698 0.00 0.00 0.00 7.698 321 GTTGTGTATAAATGTTGTGTCTTCA 53 25 0 32.00 57.246 0.00 0.00 0.00 7.754 322 TGTTGTGTATAAATGTTGTGTCTTC 52 25 0 32.00 57.246 0.00 0.00 0.00 7.754 323 TAAACATGTGTGGTATTGTGCATATA 101 26 0 30.77 58.208 0.00 0.00 0.00 7.792 324 TATGGATGTAGCTTCTTGACTGTGTG 140 26 0 42.31 61.796 0.00 0.00 0.00 7.796 325 TGTATGGATGTAGCTTCTTGACTGTG 138 26 0 42.31 61.796 0.00 0.00 0.00 7.796 326 TGTGTATGGATGTAGCTTCTTGACTG 136 26 0 42.31 61.796 0.00 0.00 0.00 7.796 327 GTATGGATGTAGCTTCTTGACTGTGT 139 26 0 42.31 61.797 0.00 0.00 0.00 7.797 328 GTGTATGGATGTAGCTTCTTGACTGT 137 26 0 42.31 61.797 0.00 0.00 0.00 7.797 329 TGGGTGTGTCTGTTAAACATGTGTG 88 25 0 44.00 62.827 0.00 0.00 0.00 7.827 330 TGTGGGTGTGTCTGTTAAACATGTG 86 25 0 44.00 62.827 0.00 0.00 0.00 7.827 331 TTGTGCATATACATGTGTGTTGTGTAT 116 27 0 33.33 60.828 4.13 3.79 41.36 7.828 332 ATTGTGCATATACATGTGTGTTGTGTA 115 27 0 33.33 60.828 3.23 0.00 40.30 7.828 333 TATTGTGCATATACATGTGTGTTGTGT 114 27 0 33.33 60.828 4.13 0.00 40.30 7.828 334 GGTGTGTCTGTTAAACATGTGTGGT 90 25 0 44.00 62.829 0.00 0.00 0.00 7.829 335 GTGGGTGTGTCTGTTAAACATGTGT 87 25 0 44.00 62.829 0.00 0.00 0.00 7.829 336 AAACATGTGTGGTATTGTGCATATACA 102 27 0 33.33 60.831 18.75 7.54 0.00 7.831 337 ATGTGTGTTGTGTATGGATGTAGCTT 128 26 0 38.46 61.979 0.00 0.00 0.00 7.979 338 GCATATACATGTGTGTTGTGTATGGAT 120 27 0 37.04 60.986 17.56 0.00 44.43 7.986 339 TGTTAAACATGTGTGGTATTGTGCATA 98 27 0 33.33 60.991 0.00 0.00 0.00 7.991 340 GAACATGTGTGGCATTGTGTATACAC 21 26 0 42.31 62.039 26.66 26.66 41.05 8.039 341 ATGTGTGGTATTGTGCATATACATGTG 106 27 0 37.04 61.200 9.61 0.00 34.94 8.200 342 CATGTGTGGTATTGTGCATATACATGT 105 27 0 37.04 61.200 15.63 3.20 34.94 8.200 343 ACATGTGTGGTATTGTGCATATACATG 104 27 0 37.04 61.200 17.52 16.48 36.76 8.200 344 TTAAACATGTGTGGTATTGTGCATATA 100 27 0 29.63 58.771 0.00 0.00 0.00 8.229 345 GTGTGTTGTGTATGGATGTAGCTTCT 130 26 0 42.31 62.236 0.00 0.00 0.00 8.236 346 TATGTGTTGTGTATAAATGTTGTGTCT 48 27 0 29.63 58.667 0.00 0.00 0.00 8.333 347 TGTGCATATACATGTGTGTTGTGTATG 117 27 0 37.04 61.352 13.84 13.84 45.64 8.352 348 TGGTATTGTGCATATACATGTGTGTTG 111 27 0 37.04 61.358 18.75 0.00 40.30 8.358 349 GGTATTGTGCATATACATGTGTGTTGT 112 27 0 37.04 61.358 18.75 0.00 40.30 8.358 350 GTGGTATTGTGCATATACATGTGTGTT 110 27 0 37.04 61.358 9.41 0.00 40.30 8.358 351 TGTCTGTTAAACATGTGTGGTATTGTG 94 27 0 37.04 61.410 0.00 0.00 0.00 8.410 352 TGTGTCTGTTAAACATGTGTGGTATTG 92 27 0 37.04 61.410 0.00 0.00 0.00 8.410 353 GTGTCTGTTAAACATGTGTGGTATTGT 93 27 0 37.04 61.410 0.00 0.00 0.00 8.410 354 GTGTGTCTGTTAAACATGTGTGGTATT 91 27 0 37.04 61.410 0.00 0.00 0.00 8.410 355 TGTGTGTTGTGTATGGATGTAGCTTC 129 26 0 42.31 62.452 0.00 0.00 0.00 8.452 356 CATGTGTGTTGTGTATGGATGTAGCT 127 26 0 42.31 62.512 0.00 0.00 0.00 8.512 357 TATGTGTTGTGTATAAATGTTGTGTC 48 26 0 30.77 57.465 0.00 0.00 0.00 8.535 358 GGTGTGTCTGTTAAACATGTGTGGTA 90 26 0 42.31 62.567 0.00 0.00 0.00 8.567 359 GATGTAGCTTCTTGACTGTGTGTTGT 144 26 0 42.31 62.610 0.00 0.00 0.00 8.610 360 ACATGTGTGTTGTGTATGGATGTAGC 126 26 0 42.31 62.728 0.00 0.00 34.85 8.728 361 TCTTGTGGGTGTGTCTGTTAAACATG 83 26 0 42.31 62.792 0.00 0.00 0.00 8.792 362 GTCTTGTGGGTGTGTCTGTTAAACAT 82 26 0 42.31 62.793 0.00 0.00 0.00 8.793 363 ATGTAGCTTCTTGACTGTGTGTTGTG 145 26 0 42.31 62.828 0.00 0.00 0.00 8.828 364 GTGCATATACATGTGTGTTGTGTATGG 118 27 0 40.74 61.873 17.56 6.84 44.43 8.873 365 GTGTGGTATTGTGCATATACATGTGTG 108 27 0 40.74 61.873 8.22 4.60 34.94 8.873 366 CATATGTGTTGTGTATAAATGTTGTGT 46 27 0 29.63 58.095 0.00 0.00 0.00 8.905 367 ACATATGTGTTGTGTATAAATGTTGTG 45 27 0 29.63 58.095 8.19 0.00 34.85 8.905 368 CACATATGTGTTGTGTATAAATGTTGT 44 27 0 29.63 58.095 25.59 0.00 41.45 8.905 369 CTGTTAAACATGTGTGGTATTGTGCAT 97 27 0 37.04 61.943 0.00 0.00 0.00 8.943 370 TGCATATACATGTGTGTTGTGTATGGA 119 27 0 37.04 62.020 17.56 14.16 44.43 9.020 371 ATATGTGTTGTGTATAAATGTTGTGTC 47 27 0 29.63 57.879 0.00 0.00 0.00 9.121 372 TTGTGTATGGATGTAGCTTCTTGACTG 135 27 0 40.74 62.221 0.00 0.00 0.00 9.221 373 GTTGTGTATGGATGTAGCTTCTTGACT 134 27 0 40.74 62.222 0.00 0.00 0.00 9.222 374 TGTGGTATTGTGCATATACATGTGTGT 109 27 0 37.04 62.236 9.41 0.00 42.66 9.236 375 TGTGTGGTATTGTGCATATACATGTGT 107 27 0 37.04 62.236 8.22 0.00 34.94 9.236 376 GTCTGTTAAACATGTGTGGTATTGTGC 95 27 0 40.74 62.394 0.00 0.00 0.00 9.394 377 TGTTGTGTATGGATGTAGCTTCTTGAC 133 27 0 40.74 62.430 0.00 0.00 0.00 9.430 378 GTGTTGTGTATGGATGTAGCTTCTTGA 132 27 0 40.74 62.430 0.00 0.00 0.00 9.430 379 TACATGTGTGTTGTGTATGGATGTAGC 125 27 0 40.74 62.480 9.61 0.00 40.30 9.480 380 TGTGTTGTGTATGGATGTAGCTTCTTG 131 27 0 40.74 62.643 0.00 0.00 0.00 9.643 381 GTGTGTTGTGTATGGATGTAGCTTCTT 130 27 0 40.74 62.644 0.00 0.00 0.00 9.644 382 ATGTGTGTTGTGTATGGATGTAGCTTC 128 27 0 40.74 62.698 0.00 0.00 0.00 9.698 383 GTATGGATGTAGCTTCTTGACTGTGTG 139 27 0 44.44 62.728 0.00 0.00 0.00 9.728 384 GTGTATGGATGTAGCTTCTTGACTGTG 137 27 0 44.44 62.728 0.00 0.00 0.00 9.728 385 TCTGTTAAACATGTGTGGTATTGTGCA 96 27 0 37.04 62.765 0.00 0.00 0.00 9.765 386 GGTGTGTCTGTTAAACATGTGTGGTAT 90 27 0 40.74 62.809 0.00 0.00 0.00 9.809 387 CATGTGTGTTGTGTATGGATGTAGCTT 127 27 0 40.74 62.910 0.00 0.00 0.00 9.910 primer3-2.3.6/test/primer1_th_list_last/SP01-15.txt.for0000664000077300007730000000031012231156243021411 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity primer3-2.3.6/test/primer1_th_list_last/SP04-07.txt.for0000664000077300007730000015617412231156243021441 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 CCGTGTACACACTGAACACG 48 20 0 55.00 60.033 25.52 20.62 44.85 0.033 1 CCGCCATGTACACACTGAAG 96 20 0 55.00 59.820 0.00 0.00 0.00 0.180 2 CCACCGTGTACACACTGAAC 45 20 0 55.00 59.681 21.51 0.00 44.85 0.319 3 GCCAAGGGAATGCACTGAAG 134 20 0 55.00 60.383 0.00 0.00 0.00 0.383 4 ACACACTGAACACGTTCCCT 54 20 0 50.00 60.386 7.97 0.00 39.29 0.386 5 ATGCACTGAAGACCTTCCCT 143 20 0 50.00 59.579 0.24 0.00 39.29 0.421 6 AGGGAATGCACTGAAGACCT 138 20 0 50.00 59.579 0.00 0.00 0.00 0.421 7 ACCACACACTTCCTACAGCT 191 20 0 50.00 59.512 0.00 0.00 0.00 0.488 8 CCCTACATACAGCCAAGGGA 22 20 0 55.00 59.435 0.00 0.00 38.03 0.565 9 TCCCTACATACAGCCAAGGG 21 20 0 55.00 59.435 0.00 0.00 37.28 0.565 10 CGTGTACACACTGAACACGT 49 20 0 50.00 59.337 25.52 0.16 44.85 0.663 11 GAATGTGGCCAAGGGAATGC 127 20 0 55.00 60.741 0.00 0.00 0.00 0.741 12 CCTAAGAATGTGGCCAAGGG 122 20 0 55.00 59.158 0.00 0.00 0.00 0.842 13 CCCTAAGAATGTGGCCAAGG 121 20 0 55.00 59.158 10.84 10.84 0.00 0.842 14 CACACTTCCTACAGCTGTGG 195 20 0 55.00 59.107 25.87 22.96 0.00 0.893 15 CCACACACTTCCTACAGCTG 192 20 0 55.00 59.107 13.98 13.98 0.00 0.893 16 AGAATGTGGCCAAGGGAATG 126 20 0 50.00 59.076 0.00 0.00 0.00 0.924 17 ACATACAGCCAAGGGACTGC 26 20 0 55.00 60.964 7.15 0.00 41.53 0.964 18 CGTGTACACACTGAACACGTT 49 21 0 47.62 59.974 25.52 0.00 44.85 1.026 19 CACCGTGTACACACTGAACA 46 20 0 50.00 58.972 18.87 0.00 44.85 1.028 20 ACCGTGTACACACTGAACAC 47 20 0 50.00 58.972 18.87 5.43 44.85 1.028 21 GTACACACTGAACACGTTCCC 52 21 0 52.38 60.039 7.97 0.00 39.29 1.039 22 TGCACTGAAGACCTTCCCTA 144 20 0 50.00 58.918 0.24 0.00 39.29 1.082 23 TTCCCTACATACAGCCAAGGG 20 21 0 52.38 60.102 0.00 0.00 37.28 1.102 24 CAAGGGAATGCACTGAAGACC 136 21 0 52.38 60.104 0.00 0.00 0.00 1.104 25 CCAAGGGAATGCACTGAAGAC 135 21 0 52.38 60.104 0.00 0.00 0.00 1.104 26 GAATGCACTGAAGACCTTCCC 141 21 0 52.38 59.832 0.00 0.00 39.29 1.168 27 GGAATGCACTGAAGACCTTCC 140 21 0 52.38 59.832 7.76 0.00 39.29 1.168 28 GGGAATGCACTGAAGACCTTC 139 21 0 52.38 59.832 3.48 3.48 40.16 1.168 29 ACACACTGAACACGTTCCCTA 54 21 0 47.62 60.172 7.97 0.00 39.29 1.172 30 TACACACTGAACACGTTCCCT 53 21 0 47.62 60.172 7.97 0.00 39.29 1.172 31 CGCCATGTACACACTGAAGA 97 20 0 50.00 58.826 0.00 0.00 0.00 1.174 32 CACACACTTCCTACAGCTGTG 193 21 0 52.38 59.769 18.49 9.31 35.09 1.231 33 AATGCACTGAAGACCTTCCCT 142 21 0 47.62 60.242 0.24 0.00 39.29 1.242 34 AGGGAATGCACTGAAGACCTT 138 21 0 47.62 60.242 0.00 0.00 0.00 1.242 35 AAGGGAATGCACTGAAGACCT 137 21 0 47.62 60.242 0.00 0.00 0.00 1.242 36 TGTTCCCTAAGAATGTGGCCA 117 21 0 47.62 60.242 7.04 3.46 37.34 1.242 37 AAGAATGTGGCCAAGGGAATG 125 21 0 47.62 59.755 0.00 0.00 0.00 1.245 38 CCAAGGGAATGCACTGAAGA 135 20 0 50.00 58.721 0.00 0.00 0.00 1.279 39 AATGCACTGAAGACCTTCCC 142 20 0 50.00 58.720 0.00 0.00 39.29 1.280 40 GGGAATGCACTGAAGACCTT 139 20 0 50.00 58.720 0.00 0.00 0.00 1.280 41 AAGGGAATGCACTGAAGACC 137 20 0 50.00 58.720 0.00 0.00 0.00 1.280 42 CACCGTGTACACACTGAACAC 46 21 0 52.38 60.305 18.87 5.43 44.85 1.305 43 ATACAGCCAAGGGACTGCC 28 19 0 57.89 60.321 7.15 0.00 41.53 1.321 44 TACACACTGAACACGTTCCC 53 20 0 50.00 58.679 7.97 0.00 39.29 1.321 45 AATGTGGCCAAGGGAATGC 128 19 0 52.63 59.635 0.00 0.00 0.00 1.365 46 TGCACTGAAGACCTTCCCTAA 144 21 0 47.62 59.611 0.24 0.00 39.29 1.389 47 GCCACCGTGTACACACTGAA 44 20 0 55.00 61.441 21.51 0.00 44.85 1.441 48 AATGTGGCCAAGGGAATGCA 128 20 0 50.00 61.491 0.00 0.00 0.00 1.491 49 GTTCCCTAAGAATGTGGCCA 118 20 0 50.00 58.422 7.04 3.46 37.34 1.578 50 TGTTCCCTAAGAATGTGGCC 117 20 0 50.00 58.422 0.00 0.00 37.34 1.578 51 ATGCACTGAAGACCTTCCCTA 143 21 0 47.62 59.398 0.24 0.00 39.29 1.602 52 CACACTGAACACGTTCCCTA 55 20 0 50.00 58.390 7.97 0.00 39.29 1.610 53 ACACACTTCCTACAGCTGTG 194 20 0 50.00 58.381 18.49 9.31 35.09 1.619 54 CACACACTTCCTACAGCTGT 193 20 0 50.00 58.381 25.74 25.74 0.00 1.619 55 TGTACACACTGAACACGTTCC 51 21 0 47.62 59.365 7.97 0.00 39.29 1.635 56 CCTAAGAATGTGGCCAAGGGA 122 21 0 52.38 60.661 0.00 0.00 0.00 1.661 57 TCCCTAAGAATGTGGCCAAGG 120 21 0 52.38 60.661 10.84 10.84 0.00 1.661 58 TACCACACACTTCCTACAGCT 190 21 0 47.62 59.335 0.00 0.00 0.00 1.665 59 CATACAGCCAAGGGACTGCC 27 20 0 60.00 61.672 7.15 0.00 41.53 1.672 60 AAGAATGTGGCCAAGGGAAT 125 20 0 45.00 58.317 0.00 0.00 0.00 1.683 61 CCTACATACAGCCAAGGGAC 23 20 0 55.00 58.297 0.00 0.00 0.00 1.703 62 CGCCATGTACACACTGAAGAT 97 21 0 47.62 59.292 0.00 0.00 0.00 1.708 63 TACATACAGCCAAGGGACTGC 25 21 0 52.38 60.723 7.15 0.00 41.53 1.723 64 ACACTCACTGAAGACTTCCCT 5 21 0 47.62 59.261 13.23 0.00 0.00 1.739 65 GCCAAGGGAATGCACTGAA 134 19 0 52.63 59.260 0.00 0.00 0.00 1.740 66 GTACCACACACTTCCTACAGC 189 21 0 52.38 59.226 0.00 0.00 0.00 1.774 67 CCCTACATACAGCCAAGGGAC 22 21 0 57.14 60.793 0.00 0.00 38.03 1.793 68 GGCCAAGGGAATGCACTGAA 133 20 0 55.00 61.832 0.00 0.00 0.00 1.832 69 GCCACCGTGTACACACTGA 44 19 0 57.89 60.836 21.51 0.00 44.85 1.836 70 GACTGCCACCGTGTACACA 40 19 0 57.89 60.836 25.52 4.81 0.00 1.836 71 CTGCCACCGTGTACACACT 42 19 0 57.89 60.837 13.26 0.00 44.85 1.837 72 CACACTCACTGAAGACTTCCC 4 21 0 52.38 59.154 13.23 0.00 0.00 1.846 73 ACACACTTCCTACAGCTGTGG 194 21 0 52.38 60.858 25.87 22.96 34.25 1.858 74 CCACACACTTCCTACAGCTGT 192 21 0 52.38 60.858 25.74 25.74 0.00 1.858 75 ACCACACACTTCCTACAGCTG 191 21 0 52.38 60.858 13.98 13.98 0.00 1.858 76 ATGTGGCCAAGGGAATGCA 129 19 0 52.63 60.863 0.00 0.00 0.00 1.863 77 GCCGCCATGTACACACTGAA 95 20 0 55.00 61.864 0.00 0.00 0.00 1.864 78 GTTCCCTAAGAATGTGGCCAA 118 21 0 47.62 59.132 0.00 0.00 37.34 1.868 79 CTAAGAATGTGGCCAAGGGA 123 20 0 50.00 58.125 0.00 0.00 0.00 1.875 80 TCCCTAAGAATGTGGCCAAG 120 20 0 50.00 58.125 0.00 0.00 0.00 1.875 81 CATACAGCCAAGGGACTGC 27 19 0 57.89 59.121 7.15 0.00 41.53 1.879 82 CACACTGAACACGTTCCCTAA 55 21 0 47.62 59.086 7.97 0.00 39.29 1.914 83 TGCACTGAAGACCTTCCCT 144 19 0 52.63 59.084 0.24 0.00 39.29 1.916 84 TCCCTACATACAGCCAAGGGA 21 21 0 52.38 60.953 3.84 2.61 40.81 1.953 85 GATGTTCCCTAAGAATGTGGCC 115 22 0 50.00 59.977 0.00 0.00 37.34 2.023 86 TACACACTGAACACGTTCCCTA 53 22 0 45.45 59.976 7.97 0.00 39.29 2.024 87 TGCACTGAAGACCTTCCCTAAT 144 22 0 45.45 60.039 0.24 0.00 39.29 2.039 88 ATGCACTGAAGACCTTCCCTAA 143 22 0 45.45 60.039 0.24 0.00 39.29 2.039 89 AATGCACTGAAGACCTTCCCTA 142 22 0 45.45 60.039 0.24 0.00 39.29 2.039 90 GTGGCCAAGGGAATGCAC 131 18 0 61.11 59.930 7.04 0.00 0.00 2.070 91 ATGTTCCCTAAGAATGTGGCC 116 21 0 47.62 58.919 0.00 0.00 37.34 2.081 92 TGGCCAAGGGAATGCACT 132 18 0 55.56 60.083 7.04 0.00 0.00 2.083 93 GCTAAGACATTGTGGGTGTACC 172 22 0 50.00 59.914 0.00 0.00 40.81 2.086 94 CACACTGAACACGTTCCCTAAG 55 22 0 50.00 60.110 0.00 0.00 39.29 2.110 95 GACTGCCACCGTGTACACAC 40 20 0 60.00 62.118 25.52 9.98 43.40 2.118 96 CTGCCACCGTGTACACACTG 42 20 0 60.00 62.121 18.18 12.07 44.85 2.121 97 TGCCACCGTGTACACACTG 43 19 0 57.89 61.131 18.18 12.07 44.85 2.131 98 ACTGCCACCGTGTACACAC 41 19 0 57.89 61.132 25.52 9.98 43.40 2.132 99 AGAAATGTGGTTAAGGGACCG 75 21 0 47.62 58.861 0.00 0.00 43.40 2.139 100 CTAAGAATGTGGCCAAGGGAA 123 21 0 47.62 58.850 0.00 0.00 0.00 2.150 101 TTCCCTAAGAATGTGGCCAAG 119 21 0 47.62 58.850 0.00 0.00 0.00 2.150 102 TACAGCCAAGGGACTGCC 29 18 0 61.11 59.844 7.15 0.00 41.53 2.156 103 ATACAGCCAAGGGACTGCCA 28 20 0 55.00 62.159 7.15 0.00 41.53 2.159 104 GGACTGCCACCGTGTACA 39 18 0 61.11 60.165 0.00 0.00 0.00 2.165 105 AGACTTCCCTACATACAGCCA 16 21 0 47.62 58.833 0.00 0.00 0.00 2.167 106 ACACACTGAACACGTTCCC 54 19 0 52.63 58.828 7.97 0.00 39.29 2.172 107 GTTCCCTAAGAATGTGGCCAAG 118 22 0 50.00 60.174 0.00 0.00 37.34 2.174 108 ACACTGAACACGTTCCCTAAG 56 21 0 47.62 58.809 0.00 0.00 39.29 2.191 109 ATGTGGCCAAGGGAATGCAC 129 20 0 55.00 62.193 0.00 0.00 0.00 2.193 110 TACCACACACTTCCTACAGC 190 20 0 50.00 57.795 0.00 0.00 0.00 2.205 111 GCACTGAAGACCTTCCCTAA 145 20 0 50.00 57.774 0.00 0.00 39.29 2.226 112 GGCCAAGGGAATGCACTGA 133 19 0 57.89 61.232 0.00 0.00 0.00 2.232 113 ACACTGAACACGTTCCCTAAGA 56 22 0 45.45 60.240 1.37 0.00 39.29 2.240 114 CCGCCATGTACACACTGAAGA 96 21 0 52.38 61.257 0.00 0.00 0.00 2.257 115 CTACATACAGCCAAGGGACTG 24 21 0 52.38 58.735 6.06 6.06 41.53 2.265 116 CTTCCCTACATACAGCCAAGG 19 21 0 52.38 58.726 0.00 0.00 0.00 2.274 117 GCCGCCATGTACACACTGA 95 19 0 57.89 61.281 0.00 0.00 0.00 2.281 118 ACACTCACTGAAGACTTCCC 5 20 0 50.00 57.711 13.23 0.00 0.00 2.289 119 GCCGCCATGTACACACTG 95 18 0 61.11 59.705 0.00 0.00 0.00 2.295 120 TGCCACCGTGTACACACT 43 18 0 55.56 59.689 13.26 0.00 44.85 2.311 121 ACTGCCACCGTGTACACA 41 18 0 55.56 59.689 25.52 4.81 0.00 2.311 122 AGCTAAGACATTGTGGGTGT 171 20 0 45.00 57.680 0.00 0.00 0.00 2.320 123 CCGCCATGTACACACTGAA 96 19 0 52.63 58.677 0.00 0.00 0.00 2.323 124 TAAGAATGTGGCCAAGGGAA 124 20 0 45.00 57.655 0.00 0.00 0.00 2.345 125 TTCCCTAAGAATGTGGCCAA 119 20 0 45.00 57.655 0.00 0.00 0.00 2.345 126 ACACTGAACACGTTCCCTAA 56 20 0 45.00 57.638 7.97 0.00 39.29 2.362 127 CAAGGGAATGCACTGAAGAC 136 20 0 50.00 57.614 0.00 0.00 0.00 2.386 128 GGCCAAGGGAATGCACTG 133 18 0 61.11 59.613 0.00 0.00 0.00 2.387 129 CCACCGTGTACACACTGAACA 45 21 0 52.38 61.388 21.51 0.00 44.85 2.388 130 GTACACACTGAACACGTTCC 52 20 0 50.00 57.595 7.97 0.00 39.29 2.405 131 TGTGGCCAAGGGAATGCA 130 18 0 55.56 60.409 0.00 0.00 0.00 2.409 132 GCCATGTACACACTGAAGATGT 98 22 0 45.45 59.587 0.00 0.00 0.00 2.413 133 TAAGAATGTGGCCAAGGGAATG 124 22 0 45.45 59.576 0.00 0.00 0.00 2.424 134 ACTTCCCTACATACAGCCAAGG 18 22 0 50.00 60.440 0.00 0.00 0.00 2.440 135 TACATACAGCCAAGGGACTG 25 20 0 50.00 57.554 6.06 6.06 41.53 2.446 136 TTCCCTACATACAGCCAAGG 20 20 0 50.00 57.537 0.00 0.00 0.00 2.463 137 CACTGAACACGTTCCCTAAGA 57 21 0 47.62 58.536 5.60 0.00 39.29 2.464 138 CACACTGAACACGTTCCCT 55 19 0 52.63 58.525 7.97 0.00 39.29 2.475 139 CACGTTCCCTAAGAAATGTGGT 64 22 0 45.45 59.519 8.20 0.00 39.25 2.481 140 ACACGTTCCCTAAGAAATGTGG 63 22 0 45.45 59.519 16.45 4.35 43.64 2.481 141 AAGAAATGTGGTTAAGGGACCG 74 22 0 45.45 59.515 0.00 0.00 43.40 2.485 142 AGACTTCCCTACATACAGCCAA 16 22 0 45.45 59.501 0.00 0.00 0.00 2.499 143 AAGACTTCCCTACATACAGCCA 15 22 0 45.45 59.501 0.00 0.00 0.00 2.499 144 TGGCCAAGGGAATGCACTG 132 19 0 57.89 61.535 7.04 0.00 0.00 2.535 145 GTGGCCAAGGGAATGCACT 131 19 0 57.89 61.536 7.04 0.00 0.00 2.536 146 CGCCATGTACACACTGAAGATG 97 22 0 50.00 60.557 0.00 0.00 0.00 2.557 147 GGACTGCCACCGTGTACAC 39 19 0 63.16 61.565 16.93 16.93 0.00 2.565 148 TCACACTCACTGAAGACTTCCC 3 22 0 50.00 60.568 13.23 0.00 0.00 2.568 149 CACACTCACTGAAGACTTCCCT 4 22 0 50.00 60.569 13.23 0.00 0.00 2.569 150 AAGCTAAGACATTGTGGGTGT 170 21 0 42.86 58.429 0.00 0.00 0.00 2.571 151 CACTCACTGAAGACTTCCCT 6 20 0 50.00 57.413 13.23 0.00 0.00 2.587 152 TGCCACCGTGTACACACTGA 43 20 0 55.00 62.603 21.51 1.63 44.85 2.603 153 ACTGCCACCGTGTACACACT 41 20 0 55.00 62.611 13.26 0.00 44.85 2.611 154 GTGTACACACTGAACACGTTCC 50 22 0 50.00 60.619 21.73 0.00 43.73 2.619 155 CCCTAAGAATGTGGCCAAGGG 121 21 0 57.14 61.626 20.63 20.63 0.00 2.626 156 TACCACACACTTCCTACAGCTG 190 22 0 50.00 60.633 13.98 13.98 0.00 2.633 157 GTACCACACACTTCCTACAGCT 189 22 0 50.00 60.633 0.00 0.00 0.00 2.633 158 ATGTTCCCTAAGAATGTGGCCA 116 22 0 45.45 60.645 7.04 3.46 37.34 2.645 159 GAATGCACTGAAGACCTTCC 141 20 0 50.00 57.327 7.76 0.00 39.29 2.673 160 GGAATGCACTGAAGACCTTC 140 20 0 50.00 57.327 3.48 3.48 40.16 2.673 161 GAAATGTGGTTAAGGGACCG 76 20 0 50.00 57.324 0.00 0.00 43.40 2.676 162 GTGTACACACTGAACACGTTC 50 21 0 47.62 58.323 21.73 3.46 43.73 2.677 163 CTAAGAATGTGGCCAAGGGAAT 123 22 0 45.45 59.308 0.00 0.00 0.00 2.692 164 ACACACTGAAGATGTTCCCT 105 20 0 45.00 57.306 0.00 0.00 0.00 2.694 165 GCACTGAAGACCTTCCCTAAT 145 21 0 47.62 58.297 0.00 0.00 39.29 2.703 166 CCGTGTACACACTGAACACGT 48 21 0 52.38 61.704 25.52 2.92 44.85 2.704 167 ACCGTGTACACACTGAACACG 47 21 0 52.38 61.704 20.62 20.62 44.85 2.704 168 GACTTCCCTACATACAGCCA 17 20 0 50.00 57.260 0.00 0.00 0.00 2.740 169 GCCACCGTGTACACACTG 44 18 0 61.11 59.243 18.18 12.07 44.85 2.757 170 CTGCCACCGTGTACACAC 42 18 0 61.11 59.243 25.52 9.98 43.40 2.757 171 ACACACTGAACACGTTCCCTAA 54 22 0 45.45 60.766 7.97 0.00 39.29 2.766 172 CCACCGTGTACACACTGAA 45 19 0 52.63 58.230 21.51 0.00 44.85 2.770 173 AAGGGACTGCCACCGTGTA 36 19 0 57.89 61.776 0.00 0.00 41.53 2.776 174 TACAGCCAAGGGACTGCCA 29 19 0 57.89 61.790 7.15 0.00 41.53 2.790 175 GTGGCCAAGGGAATGCACTG 131 20 0 60.00 62.801 7.04 0.00 0.00 2.801 176 CACTGAACACGTTCCCTAAGAA 57 22 0 45.45 59.197 5.60 0.00 39.29 2.803 177 TAAGAATGTGGCCAAGGGAAT 124 21 0 42.86 58.193 0.00 0.00 0.00 2.807 178 TCACACTCACTGAAGACTTCC 3 21 0 47.62 58.185 13.23 0.00 0.00 2.815 179 GCCAAGGGAATGCACTGAAGA 134 21 0 52.38 61.821 0.00 0.00 0.00 2.821 180 TGTGGCCAAGGGAATGCAC 130 19 0 57.89 61.833 0.00 0.00 0.00 2.833 181 AAGGGAATGCACTGAAGACCTT 137 22 0 45.45 60.844 0.00 0.00 0.00 2.844 182 TGTTCCCTAAGAATGTGGCCAA 117 22 0 45.45 60.844 0.00 0.00 37.34 2.844 183 GTGTACACACTGAACACGTT 50 20 0 45.00 57.153 21.73 0.00 43.73 2.847 184 ACGTTCCCTAAGAAATGTGGT 65 21 0 42.86 58.153 0.00 0.00 36.55 2.847 185 GACTTCCCTACATACAGCCAAG 17 22 0 50.00 59.127 0.00 0.00 0.00 2.873 186 AAAGCTAAGACATTGTGGGTGT 169 22 0 40.91 59.109 0.00 0.00 0.00 2.891 187 TGTACCACACACTTCCTACAGC 188 22 0 50.00 60.894 0.00 0.00 30.77 2.894 188 ACTCACTGAAGACTTCCCTACA 7 22 0 45.45 59.102 13.23 0.00 0.00 2.898 189 ACACTCACTGAAGACTTCCCTA 5 22 0 45.45 59.102 0.00 0.00 0.00 2.898 190 CGTGTACACACTGAACACGTTC 49 22 0 50.00 60.926 25.52 3.46 44.85 2.926 191 GGGACTGCCACCGTGTAC 38 18 0 66.67 60.945 0.00 0.00 35.55 2.945 192 AGATGTTCCCTAAGAATGTGGC 114 22 0 45.45 59.047 0.00 0.00 37.34 2.953 193 ACTGAACACGTTCCCTAAGA 58 20 0 45.00 57.046 5.60 0.00 39.29 2.954 194 GACTTCCCTACATACAGCCAA 17 21 0 47.62 58.024 0.00 0.00 0.00 2.976 195 ACTTCCCTACATACAGCCAAG 18 21 0 47.62 58.018 0.00 0.00 0.00 2.982 196 AGCTAAGACATTGTGGGTGTAC 171 22 0 45.45 58.991 0.00 0.00 0.00 3.009 197 ATGCACTGAAGACCTTCCC 143 19 0 52.63 57.956 0.00 0.00 39.29 3.044 198 GGGAATGCACTGAAGACCT 139 19 0 52.63 57.956 0.00 0.00 0.00 3.044 199 AGGGAATGCACTGAAGACC 138 19 0 52.63 57.956 0.00 0.00 0.00 3.044 200 GACTGCCACCGTGTACAC 40 18 0 61.11 58.934 16.93 16.93 0.00 3.066 201 TGTACACACTGAAGATGTTCCC 102 22 0 45.45 58.926 0.00 0.00 0.00 3.074 202 CCTACATACAGCCAAGGGACTG 23 22 0 54.55 61.093 6.06 6.06 41.53 3.093 203 ACCACACACTTCCTACAGC 191 19 0 52.63 57.902 0.00 0.00 0.00 3.098 204 TGTACCACACACTTCCTACAG 188 21 0 47.62 57.902 0.00 0.00 30.77 3.098 205 CTTCCCTACATACAGCCAAGGG 19 22 0 54.55 61.099 0.00 0.00 37.28 3.099 206 AAGACTTCCCTACATACAGCCAA 15 23 0 43.48 60.111 0.00 0.00 0.00 3.111 207 CACGTTCCCTAAGAAATGTGGTT 64 23 0 43.48 60.113 8.20 0.00 39.25 3.113 208 AACACGTTCCCTAAGAAATGTGG 62 23 0 43.48 60.113 16.45 4.35 43.64 3.113 209 GCCATGTACACACTGAAGATG 98 21 0 47.62 57.881 0.00 0.00 0.00 3.119 210 ATGTGGCCAAGGGAATGC 129 18 0 55.56 58.880 0.00 0.00 0.00 3.120 211 AGGGACTGCCACCGTGTA 37 18 0 61.11 61.135 0.00 0.00 37.89 3.135 212 GAAGACTTCCCTACATACAGCC 14 22 0 50.00 58.864 4.25 0.00 0.00 3.136 213 TGCACTGAAGACCTTCCCTAATA 144 23 0 43.48 59.854 0.24 0.00 39.29 3.146 214 ACGTTCCCTAAGAAATGTGGTT 65 22 0 40.91 58.845 0.00 0.00 36.55 3.155 215 GCCATGTACACACTGAAGATGTT 98 23 0 43.48 60.176 0.00 0.00 0.00 3.176 216 AGAATGTGGCCAAGGGAATGC 126 21 0 52.38 62.177 0.00 0.00 0.00 3.177 217 ACTGAACACGTTCCCTAAGAA 58 21 0 42.86 57.819 5.60 0.00 39.29 3.181 218 CCCTACATACAGCCAAGGG 22 19 0 57.89 57.806 0.00 0.00 0.00 3.194 219 CACGTTCCCTAAGAAATGTGG 64 21 0 47.62 57.805 8.20 0.00 39.25 3.195 220 CACTGAACACGTTCCCTAAGAAA 57 23 0 43.48 59.802 5.60 0.00 39.29 3.198 221 GAATGCACTGAAGACCTTCCCT 141 22 0 50.00 61.232 0.24 0.00 39.29 3.232 222 AGGGAATGCACTGAAGACCTTC 138 22 0 50.00 61.232 3.48 3.48 40.16 3.232 223 CCTAAGAATGTGGCCAAGGGAA 122 22 0 50.00 61.241 0.00 0.00 0.00 3.241 224 TTCCCTAAGAATGTGGCCAAGG 119 22 0 50.00 61.241 10.84 10.84 0.00 3.241 225 AAGAATGTGGCCAAGGGAA 125 19 0 47.37 57.758 0.00 0.00 0.00 3.242 226 CACTCACTGAAGACTTCCCTAC 6 22 0 50.00 58.740 13.23 0.00 0.00 3.260 227 AAGACTTCCCTACATACAGCC 15 21 0 47.62 57.738 0.00 0.00 0.00 3.262 228 AAAAGCTAAGACATTGTGGGTGT 168 23 0 39.13 59.730 0.00 0.00 0.00 3.270 229 ACAAAAGCTAAGACATTGTGGGT 166 23 0 39.13 59.730 0.17 0.00 0.00 3.270 230 TGTACACACTGAAGATGTTCCCT 102 23 0 43.48 60.303 0.00 0.00 0.00 3.303 231 GATCACACTCACTGAAGACTTCC 1 23 0 47.83 59.679 13.23 0.00 0.00 3.321 232 CGCCGCCATGTACACACT 94 18 0 61.11 61.326 0.00 0.00 0.00 3.326 233 GTGGTTAAGGGACCGCCG 81 18 0 66.67 61.331 0.00 0.00 43.60 3.331 234 AAGATGTTCCCTAAGAATGTGGC 113 23 0 43.48 59.668 0.00 0.00 37.34 3.332 235 ATCACACTCACTGAAGACTTCC 2 22 0 45.45 58.663 13.23 0.00 0.00 3.337 236 GTACACACTGAAGATGTTCCCT 103 22 0 45.45 58.658 0.00 0.00 0.00 3.342 237 ACATACAGCCAAGGGACTG 26 19 0 52.63 57.649 6.06 6.06 41.53 3.351 238 CACTCACTGAAGACTTCCCTACA 6 23 0 47.83 60.365 13.23 0.00 0.00 3.365 239 CACACTCACTGAAGACTTCCCTA 4 23 0 47.83 60.365 0.00 0.00 0.00 3.365 240 ACACTCACTGAAGACTTCCCTAC 5 23 0 47.83 60.365 13.23 0.00 0.00 3.365 241 AAGCTAAGACATTGTGGGTGTAC 170 23 0 43.48 59.609 0.00 0.00 0.00 3.391 242 GTACACACTGAACACGTTCCCT 52 22 0 50.00 61.406 0.00 0.00 39.29 3.406 243 ACACTTCCTACAGCTGTGG 196 19 0 52.63 57.592 25.87 22.96 0.00 3.408 244 CCACACACTTCCTACAGCT 192 19 0 52.63 57.592 0.00 0.00 0.00 3.408 245 AGCTAAGACATTGTGGGTGTA 171 21 0 42.86 57.587 0.00 0.00 0.00 3.413 246 GATGTTCCCTAAGAATGTGGC 115 21 0 47.62 57.577 0.00 0.00 37.34 3.423 247 ATGCACTGAAGACCTTCCCTAAT 143 23 0 43.48 60.433 0.24 0.00 39.29 3.433 248 GAATGTGGCCAAGGGAATGCA 127 21 0 52.38 62.445 0.00 0.00 0.00 3.445 249 ACTCACTGAAGACTTCCCTACAT 7 23 0 43.48 59.533 0.00 0.00 0.00 3.467 250 GCTAAGACATTGTGGGTGTAC 172 21 0 47.62 57.532 0.00 0.00 0.00 3.468 251 ACTGAACACGTTCCCTAAGAAA 58 22 0 40.91 58.522 5.60 0.00 39.29 3.478 252 AGAATGTGGCCAAGGGAAT 126 19 0 47.37 57.513 0.00 0.00 0.00 3.487 253 CGTGTACACACTGAACACG 49 19 0 52.63 57.510 25.52 16.92 44.85 3.490 254 GAAGACTTCCCTACATACAGCCA 14 23 0 47.83 60.493 4.25 0.00 0.00 3.493 255 TGAAGACTTCCCTACATACAGCC 13 23 0 47.83 60.493 13.23 0.00 0.00 3.493 256 AGACTTCCCTACATACAGCCAAG 16 23 0 47.83 60.494 0.00 0.00 0.00 3.494 257 CAAGGGAATGCACTGAAGACCT 136 22 0 50.00 61.495 0.00 0.00 0.00 3.495 258 AGGGACTGCCACCGTGTAC 37 19 0 63.16 62.503 0.00 0.00 37.89 3.503 259 GCCAAGGGAATGCACTGA 134 18 0 55.56 58.490 0.00 0.00 0.00 3.510 260 GCACTGAAGACCTTCCCTAATAC 145 23 0 47.83 59.486 0.00 0.00 39.29 3.514 261 TTCCCTACATACAGCCAAGGGA 20 22 0 50.00 61.528 3.84 2.80 41.15 3.528 262 ACCGTGTACACACTGAACA 47 19 0 47.37 57.458 18.87 0.00 44.85 3.542 263 GAATGTGGCCAAGGGAATG 127 19 0 52.63 57.456 0.00 0.00 0.00 3.544 264 CTAAGAATGTGGCCAAGGGAATG 123 23 0 47.83 60.553 0.00 0.00 0.00 3.553 265 TACACACTGAACACGTTCCCTAA 53 23 0 43.48 60.554 7.97 0.00 39.29 3.554 266 CAAGGGACTGCCACCGTG 35 18 0 66.67 61.569 0.00 0.00 41.53 3.569 267 CAGCCAAGGGACTGCCAC 31 18 0 66.67 61.583 0.00 0.00 41.53 3.583 268 CCGCCATGTACACACTGAAGAT 96 22 0 50.00 61.605 0.00 0.00 0.00 3.605 269 AATGCACTGAAGACCTTCCCTAA 142 23 0 43.48 60.625 0.24 0.00 39.29 3.625 270 CGCCGCCATGTACACACTG 94 19 0 63.16 62.625 0.00 0.00 0.00 3.625 271 GCCACCGTGTACACACTGAAC 44 21 0 57.14 62.629 11.38 0.96 44.85 3.629 272 CAAAAGCTAAGACATTGTGGGTG 167 23 0 43.48 59.370 0.00 0.00 0.00 3.630 273 ATGTACACACTGAAGATGTTCCC 101 23 0 43.48 59.356 0.00 0.00 0.00 3.644 274 TAAGAAATGTGGTTAAGGGACCG 73 23 0 43.48 59.355 0.00 0.00 43.40 3.645 275 AAAGCTAAGACATTGTGGGTG 169 21 0 42.86 57.353 0.00 0.00 0.00 3.647 276 TGTACACACTGAACACGTTCCC 51 22 0 50.00 61.660 0.00 0.00 39.29 3.660 277 CTCACTGAAGACTTCCCTACA 8 21 0 47.62 57.333 13.23 0.00 0.00 3.667 278 CACTCACTGAAGACTTCCCTA 6 21 0 47.62 57.333 0.00 0.00 0.00 3.667 279 ACTCACTGAAGACTTCCCTAC 7 21 0 47.62 57.331 13.23 0.00 0.00 3.669 280 CTACATACAGCCAAGGGACTGC 24 22 0 54.55 61.671 7.15 0.00 41.53 3.671 281 AAGCTAAGACATTGTGGGTGTA 170 22 0 40.91 58.307 0.00 0.00 0.00 3.693 282 AATGTGGCCAAGGGAATGCAC 128 21 0 52.38 62.720 0.00 0.00 0.00 3.720 283 GGCCAAGGGAATGCACTGAAG 133 21 0 57.14 62.759 0.00 0.00 0.00 3.759 284 CGCCATGTACACACTGAAG 97 19 0 52.63 57.239 0.00 0.00 0.00 3.761 285 CTGAAGACTTCCCTACATACAGC 12 23 0 47.83 59.238 13.23 0.00 0.00 3.762 286 GCCGCCATGTACACACTGAAG 95 21 0 57.14 62.769 0.00 0.00 0.00 3.769 287 ACACACTGAAGATGTTCCCTA 105 21 0 42.86 57.230 0.00 0.00 0.00 3.770 288 TACACACTGAAGATGTTCCCT 104 21 0 42.86 57.230 0.00 0.00 0.00 3.770 289 TGAACACGTTCCCTAAGAAATGT 60 23 0 39.13 59.227 7.97 0.00 38.27 3.773 290 GGGACTGCCACCGTGTACA 38 19 0 63.16 62.795 0.00 0.00 35.55 3.795 291 TGAAGACTTCCCTACATACAGC 13 22 0 45.45 58.193 13.23 0.00 0.00 3.807 292 ACACTGAACACGTTCCCTAAGAA 56 23 0 43.48 60.807 1.37 0.00 39.29 3.807 293 GTACACACTGAAGATGTTCCC 103 21 0 47.62 57.185 0.00 0.00 0.00 3.815 294 GCACTGAAGACCTTCCCTAATA 145 22 0 45.45 58.182 0.00 0.00 39.29 3.818 295 CCGTGTACACACTGAACAC 48 19 0 52.63 57.102 25.52 5.43 44.85 3.898 296 CACCGTGTACACACTGAAC 46 19 0 52.63 57.102 18.87 0.00 44.85 3.898 297 ACACGTTCCCTAAGAAATGTG 63 21 0 42.86 57.101 12.57 12.57 44.20 3.899 298 CCAAGGGAATGCACTGAAG 135 19 0 52.63 57.091 0.00 0.00 0.00 3.909 299 CGTTCCCTAAGAAATGTGGTT 66 21 0 42.86 57.082 0.00 0.00 36.55 3.918 300 AAAAGCTAAGACATTGTGGGTG 168 22 0 40.91 58.072 0.00 0.00 0.00 3.928 301 CAAAAGCTAAGACATTGTGGGT 167 22 0 40.91 58.072 0.00 0.00 0.00 3.928 302 ACAAAAGCTAAGACATTGTGGG 166 22 0 40.91 58.072 0.17 0.00 0.00 3.928 303 ATCACACTCACTGAAGACTTCCC 2 23 0 47.83 60.933 13.23 0.00 0.00 3.933 304 ACACACTGAAGATGTTCCCTAAG 105 23 0 43.48 59.037 0.00 0.00 0.00 3.963 305 GAATGCACTGAAGACCTTCCCTA 141 23 0 47.83 60.999 0.24 0.00 39.29 3.999 306 CCTAAGAAATGTGGTTAAGGGACC 71 24 0 45.83 59.995 0.00 0.00 40.50 4.005 307 CCCTAAGAAATGTGGTTAAGGGAC 70 24 0 45.83 59.995 0.00 0.00 32.66 4.005 308 ACTGAACACGTTCCCTAAGAAAT 58 23 0 39.13 58.970 5.60 0.00 39.29 4.030 309 ACACACTGAAGATGTTCCCTAA 105 22 0 40.91 57.968 0.00 0.00 0.00 4.032 310 AAAGCTAAGACATTGTGGGTGTA 169 23 0 39.13 58.964 0.00 0.00 0.00 4.036 311 CACACACTTCCTACAGCTGTGG 193 22 0 54.55 62.046 25.87 22.96 34.25 4.046 312 CCACACACTTCCTACAGCTGTG 192 22 0 54.55 62.046 18.49 9.31 35.09 4.046 313 CACGTTCCCTAAGAAATGTGGTTA 64 24 0 41.67 59.936 8.20 0.00 39.25 4.064 314 GACCGCCGCCATGTACAC 91 18 0 66.67 62.075 0.00 0.00 0.00 4.075 315 AAGGGACTGCCACCGTGT 36 18 0 61.11 62.100 0.00 0.00 41.53 4.100 316 CCGCCATGTACACACTGA 96 18 0 55.56 57.888 0.00 0.00 0.00 4.112 317 TCCCTAAGAAATGTGGTTAAGGGA 69 24 0 41.67 60.115 0.00 0.00 35.42 4.115 318 TGTACACACTGAAGATGTTCCCTA 102 24 0 41.67 60.116 0.00 0.00 0.00 4.116 319 GAACACGTTCCCTAAGAAATGTG 61 23 0 43.48 58.882 12.57 12.57 44.20 4.118 320 ACAGCCAAGGGACTGCCA 30 18 0 61.11 62.118 7.15 0.00 41.53 4.118 321 ACACGTTCCCTAAGAAATGTGGT 63 23 0 43.48 61.128 16.45 4.84 43.64 4.128 322 GGAATGCACTGAAGACCTTCCC 140 22 0 54.55 62.135 1.05 0.00 39.29 4.135 323 GGGAATGCACTGAAGACCTTCC 139 22 0 54.55 62.135 7.76 5.36 39.29 4.135 324 CTCACTGAAGACTTCCCTACAT 8 22 0 45.45 57.852 0.00 0.00 0.00 4.148 325 GTACACACTGAACACGTTCCCTA 52 23 0 47.83 61.171 0.00 0.00 39.29 4.171 326 AACACGTTCCCTAAGAAATGTG 62 22 0 40.91 57.827 12.57 12.57 44.20 4.173 327 TCCCTACATACAGCCAAGGGAC 21 22 0 54.55 62.177 3.84 0.00 39.34 4.177 328 AAAGCTAAGACATTGTGGGTGTAC 169 24 0 41.67 60.177 0.00 0.00 0.00 4.177 329 CTGAACACGTTCCCTAAGAAATGT 59 24 0 41.67 60.177 0.00 0.00 38.27 4.177 330 ACTGAACACGTTCCCTAAGAAATG 58 24 0 41.67 60.177 5.60 0.00 39.29 4.177 331 CACTGAACACGTTCCCTAAGAAAT 57 24 0 41.67 60.177 5.60 0.00 39.29 4.177 332 ATGTTCCCTAAGAATGTGGCCAA 116 23 0 43.48 61.205 0.00 0.00 37.34 4.205 333 CCGTGTACACACTGAACACGTT 48 22 0 50.00 62.213 25.52 0.00 44.85 4.213 334 CACACTGAAGATGTTCCCTAAGA 106 23 0 43.48 58.784 0.00 0.00 0.00 4.216 335 CCCTAAGAAATGTGGTTAAGGGA 70 23 0 43.48 58.763 0.00 0.00 32.66 4.237 336 TCCCTAAGAAATGTGGTTAAGGG 69 23 0 43.48 58.763 0.00 0.00 32.26 4.237 337 ATGCACTGAAGACCTTCCCTAATA 143 24 0 41.67 60.239 0.24 0.00 39.29 4.239 338 AGCTAAGACATTGTGGGTGTACC 171 23 0 47.83 61.250 0.00 0.00 40.81 4.250 339 ACGTTCCCTAAGAAATGTGGTTA 65 23 0 39.13 58.711 0.00 0.00 36.55 4.289 340 CTAAGAAATGTGGTTAAGGGACCG 72 24 0 45.83 60.297 0.00 0.00 43.40 4.297 341 AGATGTTCCCTAAGAATGTGGCC 114 23 0 47.83 61.322 0.00 0.00 37.34 4.322 342 TTAAGGGACCGCCGCCAT 85 18 0 61.11 62.333 0.00 0.00 34.23 4.333 343 ACACACTGAAGATGTTCCCTAAGA 105 24 0 41.67 60.361 0.00 0.00 0.00 4.361 344 CTGAACACGTTCCCTAAGAAATG 59 23 0 43.48 58.631 7.97 0.00 39.29 4.369 345 GACTTCCCTACATACAGCCAAGG 17 23 0 52.17 61.371 0.00 0.00 0.00 4.371 346 TCACTGAAGACTTCCCTACATACA 9 24 0 41.67 59.624 13.23 0.00 0.00 4.376 347 CCGCCGCCATGTACACAC 93 18 0 66.67 62.378 0.00 0.00 0.00 4.378 348 CCAAGGGAATGCACTGAAGACC 135 22 0 54.55 62.394 0.00 0.00 0.00 4.394 349 TGAACACGTTCCCTAAGAAATGTG 60 24 0 41.67 60.417 12.57 12.57 44.20 4.417 350 CACACTGAACACGTTCCCTAAGA 55 23 0 47.83 61.421 1.37 0.00 39.29 4.421 351 AAAAGCTAAGACATTGTGGGTGTA 168 24 0 37.50 59.566 0.00 0.00 0.00 4.434 352 TACAAAAGCTAAGACATTGTGGGT 165 24 0 37.50 59.566 8.27 0.00 0.00 4.434 353 TGAACACGTTCCCTAAGAAATG 60 22 0 40.91 57.565 7.97 0.00 39.29 4.435 354 GAACACGTTCCCTAAGAAATGT 61 22 0 40.91 57.563 0.00 0.00 38.27 4.437 355 GTACACACTGAAGATGTTCCCTA 103 23 0 43.48 58.533 0.00 0.00 0.00 4.467 356 ATACAGCCAAGGGACTGC 28 18 0 55.56 57.518 7.15 0.00 41.53 4.482 357 ACCACACACTTCCTACAGCTGT 191 22 0 50.00 62.496 25.74 25.74 0.00 4.496 358 CCACCGTGTACACACTGAACAC 45 22 0 54.55 62.531 21.51 5.43 44.85 4.531 359 CATGTACACACTGAAGATGTTCCC 100 24 0 45.83 60.535 0.00 0.00 0.00 4.535 360 CCATGTACACACTGAAGATGTTCC 99 24 0 45.83 60.535 0.00 0.00 0.00 4.535 361 TGAAGATGTTCCCTAAGAATGTGG 111 24 0 41.67 59.445 0.00 0.00 37.34 4.555 362 GATGTTCCCTAAGAATGTGGCCA 115 23 0 47.83 61.573 7.04 3.46 37.34 4.573 363 ACACTGAAGATGTTCCCTAAGA 107 22 0 40.91 57.422 0.00 0.00 0.00 4.578 364 CCACCGTGTACACACTGA 45 18 0 55.56 57.417 21.51 0.00 44.85 4.583 365 CCTAAGAATGTGGCCAAGGGAAT 122 23 0 47.83 61.584 0.00 0.00 0.00 4.584 366 GAAGATGTTCCCTAAGAATGTGGC 112 24 0 45.83 60.598 0.00 0.00 37.34 4.598 367 GATCACACTCACTGAAGACTTC 1 22 0 45.45 57.400 8.93 8.93 0.00 4.600 368 CACACTGAAGATGTTCCCTAAGAA 106 24 0 41.67 59.389 0.00 0.00 0.00 4.611 369 CATGTACACACTGAAGATGTTCC 100 23 0 43.48 58.379 0.00 0.00 0.00 4.621 370 CCATGTACACACTGAAGATGTTC 99 23 0 43.48 58.379 0.00 0.00 0.00 4.621 371 TTCCCTAAGAAATGTGGTTAAGGG 68 24 0 41.67 59.378 0.00 0.00 32.26 4.622 372 ACTCACTGAAGACTTCCCTACATA 7 24 0 41.67 59.375 0.00 0.00 0.00 4.625 373 CACACTGAAGATGTTCCCTAAG 106 22 0 45.45 57.369 0.00 0.00 0.00 4.631 374 TGCACTGAAGACCTTCCC 144 18 0 55.56 57.361 0.00 0.00 39.29 4.639 375 ATGTACACACTGAAGATGTTCCCT 101 24 0 41.67 60.668 0.00 0.00 0.00 4.668 376 ACACACTGAACACGTTCCCTAAG 54 23 0 47.83 61.670 0.00 0.00 39.29 4.670 377 CCCTAAGAAATGTGGTTAAGGG 70 22 0 45.45 57.326 0.00 0.00 0.00 4.674 378 ACGTTCCCTAAGAAATGTGGTTAA 65 24 0 37.50 59.323 0.00 0.00 36.55 4.677 379 AAGAATGTGGCCAAGGGAATGC 125 22 0 50.00 62.682 0.00 0.00 0.00 4.682 380 CTGAACACGTTCCCTAAGAAAT 59 22 0 40.91 57.294 7.97 0.00 39.29 4.706 381 CCATGTACACACTGAAGATGTT 99 22 0 40.91 57.289 0.00 0.00 0.00 4.711 382 CACTCACTGAAGACTTCCCTACAT 6 24 0 45.83 60.724 0.00 0.00 0.00 4.724 383 CACTGAAGACTTCCCTACATACAG 10 24 0 45.83 59.276 4.00 0.00 0.00 4.724 384 CACTGAAGACTTCCCTACATACA 10 23 0 43.48 58.275 13.23 0.00 0.00 4.725 385 CACTGAAGACCTTCCCTAATACA 146 23 0 43.48 58.264 7.76 0.00 39.29 4.736 386 AAGGGAATGCACTGAAGACCTTC 137 23 0 47.83 61.757 3.48 3.48 40.16 4.757 387 TGTTCCCTAAGAATGTGGCCAAG 117 23 0 47.83 61.761 0.00 0.00 37.34 4.761 388 ACTGAAGACTTCCCTACATACAGC 11 24 0 45.83 60.784 4.66 0.00 0.00 4.784 389 GTACCACACACTTCCTACAGCTG 189 23 0 52.17 61.787 13.98 13.98 0.00 4.787 390 CACCGTGTACACACTGAACACG 46 22 0 54.55 62.812 20.62 20.62 44.85 4.812 391 AGACCTTCCCTAATACAAAAGCT 152 23 0 39.13 58.173 0.00 0.00 0.00 4.827 392 TACACACTGAAGATGTTCCCTA 104 22 0 40.91 57.160 0.00 0.00 0.00 4.840 393 GTACACACTGAAGATGTTCCCTAA 103 24 0 41.67 59.148 0.00 0.00 0.00 4.852 394 ACACTGAAGATGTTCCCTAAGAA 107 23 0 39.13 58.122 0.00 0.00 0.00 4.878 395 CCAAGGGACTGCCACCGT 34 18 0 66.67 62.878 0.00 0.00 41.53 4.878 396 CAAAAGCTAAGACATTGTGGGTGT 167 24 0 41.67 60.900 0.00 0.00 0.00 4.900 397 ACAAAAGCTAAGACATTGTGGGTG 166 24 0 41.67 60.900 0.17 0.00 0.00 4.900 398 TCACACTCACTGAAGACTTCCCT 3 23 0 47.83 61.901 13.23 0.00 0.00 4.901 399 AGCCAAGGGACTGCCACC 32 18 0 66.67 62.905 0.00 0.00 41.53 4.905 400 ACCGCCGCCATGTACACA 92 18 0 61.11 62.923 0.00 0.00 0.00 4.923 401 GCCAAGGGAATGCACTGAAGAC 134 22 0 54.55 62.957 0.00 0.00 0.00 4.957 402 CTCACTGAAGACTTCCCTACATAC 8 24 0 45.83 59.035 0.00 0.00 0.00 4.965 403 GCACTGAAGACCTTCCCT 145 18 0 55.56 57.027 0.00 0.00 39.29 4.973 404 ATGTACACACTGAAGATGTTCC 101 22 0 40.91 57.024 0.00 0.00 0.00 4.976 405 AATGCACTGAAGACCTTCCCTAAT 142 24 0 41.67 60.979 0.24 0.00 39.29 4.979 406 CGTTCCCTAAGAAATGTGGTTA 66 22 0 40.91 57.020 0.00 0.00 36.55 4.980 407 TCACTGAAGACTTCCCTACATAC 9 23 0 43.48 58.020 13.23 0.00 0.00 4.980 408 ACTGAAGACTTCCCTACATACAG 11 23 0 43.48 58.015 4.00 0.00 0.00 4.985 409 CCCTAAGAATGTGGCCAAGGGA 121 22 0 54.55 62.988 27.33 4.89 33.28 4.988 410 TCCCTAAGAATGTGGCCAAGGG 120 22 0 54.55 62.988 25.97 25.97 32.80 4.988 411 TGAAGACCTTCCCTAATACAAAAGC 149 25 0 40.00 60.003 7.76 0.00 39.29 5.003 412 CAAGGGAATGCACTGAAGACCTT 136 23 0 47.83 62.008 0.00 0.00 0.00 5.008 413 GAACACGTTCCCTAAGAAATGTGG 61 24 0 45.83 61.009 16.45 4.35 43.64 5.009 414 TACAAAAGCTAAGACATTGTGGG 165 23 0 39.13 57.972 8.27 0.00 0.00 5.028 415 GCACTGAAGACCTTCCCTAATACA 145 24 0 45.83 61.028 0.00 0.00 39.29 5.028 416 TGCACTGAAGACCTTCCCTAATAC 144 24 0 45.83 61.028 0.24 0.00 39.29 5.028 417 GAAGACTTCCCTACATACAGCCAA 14 24 0 45.83 61.028 4.25 0.00 0.00 5.028 418 AAGACTTCCCTACATACAGCCAAG 15 24 0 45.83 61.030 0.00 0.00 0.00 5.030 419 ATACAAAAGCTAAGACATTGTGGGT 164 25 0 36.00 59.943 8.27 0.00 0.00 5.057 420 GTACACACTGAAGATGTTCCCTAAG 103 25 0 44.00 60.064 0.00 0.00 0.00 5.064 421 GCCATGTACACACTGAAGATGTTC 98 24 0 45.83 61.065 0.00 0.00 0.00 5.065 422 CGCCATGTACACACTGAAGATGT 97 23 0 47.83 62.095 0.00 0.00 0.00 5.095 423 TACACACTGAAGATGTTCCCTAAG 104 24 0 41.67 58.902 0.00 0.00 0.00 5.098 424 CACTGAAGACCTTCCCTAATACAA 146 24 0 41.67 58.895 7.76 0.00 39.29 5.105 425 TACACACTGAAGATGTTCCCTAA 104 23 0 39.13 57.870 0.00 0.00 0.00 5.130 426 GAAGATGTTCCCTAAGAATGTGG 112 23 0 43.48 57.850 0.00 0.00 37.34 5.150 427 TACACACTGAAGATGTTCCCTAAGA 104 25 0 40.00 60.179 0.00 0.00 0.00 5.179 428 AAGACCTTCCCTAATACAAAAGCT 151 24 0 37.50 58.816 0.00 0.00 0.00 5.184 429 TACCACACACTTCCTACAGCTGT 190 23 0 47.83 62.210 25.74 25.74 0.00 5.210 430 TGTACCACACACTTCCTACAGCT 188 23 0 47.83 62.210 0.00 0.00 30.77 5.210 431 CTAAGAAATGTGGTTAAGGGACC 72 23 0 43.48 57.779 0.00 0.00 40.50 5.221 432 CCTAAGAAATGTGGTTAAGGGAC 71 23 0 43.48 57.779 0.00 0.00 0.00 5.221 433 ACTGAAGATGTTCCCTAAGAATGTG 109 25 0 40.00 59.770 0.00 0.00 37.34 5.230 434 CACTGAAGATGTTCCCTAAGAATGT 108 25 0 40.00 59.770 0.00 0.00 37.34 5.230 435 ACACTGAAGATGTTCCCTAAGAATG 107 25 0 40.00 59.770 0.00 0.00 37.34 5.230 436 CACACTGAAGATGTTCCCTAAGAAT 106 25 0 40.00 59.770 0.00 0.00 37.34 5.230 437 GAAGACCTTCCCTAATACAAAAGCT 150 25 0 40.00 59.768 0.00 0.00 34.16 5.232 438 ACGTTCCCTAAGAAATGTGGTTAAG 65 25 0 40.00 60.237 0.00 0.00 36.55 5.237 439 CTCACTGAAGACTTCCCTACATA 8 23 0 43.48 57.760 0.00 0.00 0.00 5.240 440 CGTTCCCTAAGAAATGTGGTTAAG 66 24 0 41.67 58.745 0.00 0.00 36.55 5.255 441 CGTTCCCTAAGAAATGTGGTTAA 66 23 0 39.13 57.723 0.00 0.00 36.55 5.277 442 ACACTGAACACGTTCCCTAAGAAA 56 24 0 41.67 61.327 1.37 0.00 39.29 5.327 443 CTGAAGATGTTCCCTAAGAATGTGG 110 25 0 44.00 60.353 0.00 0.00 37.34 5.353 444 GTTCCCTAAGAATGTGGCCAAGG 118 23 0 52.17 62.379 10.84 10.84 37.34 5.379 445 CTGAAGACTTCCCTACATACAGCC 12 24 0 50.00 61.381 13.23 0.00 0.00 5.381 446 TAAGAATGTGGCCAAGGGAATGC 124 23 0 47.83 62.390 0.00 0.00 0.00 5.390 447 ACTGAAGACCTTCCCTAATACAA 147 23 0 39.13 57.591 7.76 0.00 39.29 5.409 448 CTTCCCTACATACAGCCAAGGGA 19 23 0 52.17 62.417 0.00 0.00 41.15 5.417 449 ACTGAAGATGTTCCCTAAGAATGT 109 24 0 37.50 58.574 0.00 0.00 37.34 5.426 450 ACACTGAAGATGTTCCCTAAGAAT 107 24 0 37.50 58.574 0.00 0.00 37.34 5.426 451 TACACACTGAACACGTTCCCTAAG 53 24 0 45.83 61.433 0.00 0.00 39.29 5.433 452 CACGTTCCCTAAGAAATGTGGTTAA 64 25 0 40.00 60.468 8.20 0.00 39.25 5.468 453 ATGTACACACTGAAGATGTTCCCTA 101 25 0 40.00 60.474 0.00 0.00 0.00 5.474 454 CACACTCACTGAAGACTTCCCTAC 4 24 0 50.00 61.492 13.23 0.00 0.00 5.492 455 GAAGACCTTCCCTAATACAAAAGC 150 24 0 41.67 58.492 0.00 0.00 34.16 5.508 456 GAATGCACTGAAGACCTTCCCTAA 141 24 0 45.83 61.513 0.24 0.00 39.29 5.513 457 CACTGAAGACCTTCCCTAATACAAA 146 25 0 40.00 59.475 7.76 0.00 39.29 5.525 458 GTTCCCTAAGAAATGTGGTTAAGGG 67 25 0 44.00 60.528 0.00 0.00 36.55 5.528 459 TCACTGAAGACTTCCCTACATACAG 9 25 0 44.00 60.529 4.00 0.00 0.00 5.529 460 CTCACTGAAGACTTCCCTACATACA 8 25 0 44.00 60.529 0.00 0.00 0.00 5.529 461 CACTCACTGAAGACTTCCCTACATA 6 25 0 44.00 60.529 0.00 0.00 0.00 5.529 462 ACTCACTGAAGACTTCCCTACATAC 7 25 0 44.00 60.529 0.00 0.00 0.00 5.529 463 AAGACCTTCCCTAATACAAAAGC 151 23 0 39.13 57.441 0.00 0.00 0.00 5.559 464 GTTCCCTAAGAAATGTGGTTAAGG 67 24 0 41.67 58.424 0.00 0.00 36.55 5.576 465 ATACAAAAGCTAAGACATTGTGGG 164 24 0 37.50 58.421 8.27 0.00 0.00 5.579 466 ACACGTTCCCTAAGAAATGTGGTT 63 24 0 41.67 61.638 16.45 0.38 43.64 5.638 467 AACACGTTCCCTAAGAAATGTGGT 62 24 0 41.67 61.638 16.45 10.25 43.64 5.638 468 TCACACTCACTGAAGACTTCCCTA 3 24 0 45.83 61.649 0.00 0.00 0.00 5.649 469 TGTACACACTGAAGATGTTCCCTAA 102 25 0 40.00 60.649 0.00 0.00 0.00 5.649 470 TTCCCTAAGAAATGTGGTTAAGGGA 68 25 0 40.00 60.657 0.00 0.00 35.73 5.657 471 TTCCCTACATACAGCCAAGGGAC 20 23 0 52.17 62.666 3.92 0.00 42.20 5.666 472 GTACACACTGAACACGTTCCCTAA 52 24 0 45.83 61.669 0.00 0.00 39.29 5.669 473 ACTTCCCTACATACAGCCAAGGG 18 23 0 52.17 62.672 0.00 0.00 37.28 5.672 474 CCGCCATGTACACACTGAAGATG 96 23 0 52.17 62.694 0.00 0.00 0.00 5.694 475 CAAAAGCTAAGACATTGTGGGTGTA 167 25 0 40.00 60.700 0.00 0.00 0.00 5.700 476 TACAAAAGCTAAGACATTGTGGGTG 165 25 0 40.00 60.700 8.27 0.00 0.00 5.700 477 AAAAGCTAAGACATTGTGGGTGTAC 168 25 0 40.00 60.700 0.00 0.00 0.00 5.700 478 GTGTACACACTGAACACGTTCCC 50 23 0 52.17 62.741 21.73 0.00 43.73 5.741 479 ACTGAAGACCTTCCCTAATACAAA 147 24 0 37.50 58.259 7.76 0.00 39.29 5.741 480 CTGAAGATGTTCCCTAAGAATGTG 110 24 0 41.67 58.254 0.00 0.00 37.34 5.746 481 CACTGAAGATGTTCCCTAAGAATG 108 24 0 41.67 58.254 0.00 0.00 37.34 5.746 482 AAGCTAAGACATTGTGGGTGTACC 170 24 0 45.83 61.752 0.00 0.00 40.81 5.752 483 AATGCACTGAAGACCTTCCCTAATA 142 25 0 40.00 60.772 0.00 0.00 39.29 5.772 484 GATCACACTCACTGAAGACTTCCC 1 24 0 50.00 61.791 13.23 0.00 0.00 5.791 485 TGAAGATGTTCCCTAAGAATGTG 111 23 0 39.13 57.198 0.00 0.00 37.34 5.802 486 CGTTCCCTAAGAAATGTGGTTAAGG 66 25 0 44.00 60.812 0.00 0.00 36.55 5.812 487 AAGATGTTCCCTAAGAATGTGGCC 113 24 0 45.83 61.826 0.00 0.00 37.34 5.826 488 ACACACTGAAGATGTTCCCTAAGAA 105 25 0 40.00 60.886 0.00 0.00 0.00 5.886 489 TTCCCTAAGAAATGTGGTTAAGG 68 23 0 39.13 57.107 0.00 0.00 0.00 5.893 490 ACACTCACTGAAGACTTCCCTACA 5 24 0 45.83 61.893 13.23 0.00 0.00 5.893 491 CACACTGAACACGTTCCCTAAGAA 55 24 0 45.83 61.909 1.37 0.00 39.29 5.909 492 AGACCTTCCCTAATACAAAAGCTA 152 24 0 37.50 58.071 0.00 0.00 0.00 5.929 493 TGTACACACTGAACACGTTCCCT 51 23 0 47.83 62.940 0.00 0.00 39.29 5.940 494 AATACAAAAGCTAAGACATTGTGGG 163 25 0 36.00 59.016 8.27 0.00 0.00 5.984 495 CACTGAAGACCTTCCCTAATACAAAA 146 26 0 38.46 60.011 7.76 0.00 39.29 6.011 496 TGAAGACTTCCCTACATACAGCCA 13 24 0 45.83 62.023 13.23 0.00 0.00 6.023 497 GATGTTCCCTAAGAATGTGGCCAA 115 24 0 45.83 62.064 0.00 0.00 37.34 6.064 498 ATGTTCCCTAAGAATGTGGCCAAG 116 24 0 45.83 62.068 0.00 0.00 37.34 6.068 499 ACTGAAGACCTTCCCTAATACAAAA 147 25 0 36.00 58.872 7.76 0.00 39.29 6.128 500 CCTTCCCTAATACAAAAGCTAAGACA 155 26 0 38.46 59.842 0.00 0.00 0.00 6.158 501 ACCTTCCCTAATACAAAAGCTAAGAC 154 26 0 38.46 59.841 0.00 0.00 0.00 6.159 502 ATCACACTCACTGAAGACTTCCCT 2 24 0 45.83 62.204 13.23 0.00 0.00 6.204 503 ACTGAAGACCTTCCCTAATACAAAAG 147 26 0 38.46 59.785 0.00 0.00 39.29 6.215 504 TCCCTAAGAAATGTGGTTAAGGGAC 69 25 0 44.00 61.241 0.00 0.00 34.20 6.241 505 CTGAACACGTTCCCTAAGAAATGTG 59 25 0 44.00 61.260 12.57 12.57 44.20 6.260 506 CACTGAACACGTTCCCTAAGAAATG 57 25 0 44.00 61.260 5.60 0.00 39.29 6.260 507 ACCTTCCCTAATACAAAAGCTAAGA 154 25 0 36.00 58.693 0.00 0.00 0.00 6.307 508 AGACCTTCCCTAATACAAAAGCTAA 152 25 0 36.00 58.693 0.00 0.00 0.00 6.307 509 AAGACCTTCCCTAATACAAAAGCTA 151 25 0 36.00 58.693 0.00 0.00 0.00 6.307 510 ATGCACTGAAGACCTTCCCTAATAC 143 25 0 44.00 61.350 0.24 0.00 39.29 6.350 511 GACCTTCCCTAATACAAAAGCTAAGA 153 26 0 38.46 59.616 0.00 0.00 0.00 6.384 512 GAAGACCTTCCCTAATACAAAAGCTA 150 26 0 38.46 59.616 0.00 0.00 34.16 6.384 513 AGACCTTCCCTAATACAAAAGCTAAG 152 26 0 38.46 59.614 0.00 0.00 0.00 6.386 514 ACACGTTCCCTAAGAAATGTGGTTA 63 25 0 40.00 61.407 16.45 0.00 43.64 6.407 515 AATACAAAAGCTAAGACATTGTGGGT 163 26 0 34.62 60.463 8.27 0.00 0.00 6.463 516 GCACTGAAGACCTTCCCTAATACAA 145 25 0 44.00 61.521 0.00 0.00 39.29 6.521 517 CGCCATGTACACACTGAAGATGTT 97 24 0 45.83 62.553 0.00 0.00 0.00 6.553 518 CCTTCCCTAATACAAAAGCTAAGAC 155 25 0 40.00 58.380 0.00 0.00 0.00 6.620 519 GACCTTCCCTAATACAAAAGCTAAG 153 25 0 40.00 58.380 0.00 0.00 0.00 6.620 520 AGACTTCCCTACATACAGCCAAGG 16 24 0 50.00 62.625 0.00 0.00 0.00 6.625 521 GACCTTCCCTAATACAAAAGCTAA 153 24 0 37.50 57.369 0.00 0.00 0.00 6.631 522 ACTGAACACGTTCCCTAAGAAATGT 58 25 0 40.00 61.636 0.00 0.00 38.27 6.636 523 ACACTGAACACGTTCCCTAAGAAAT 56 25 0 40.00 61.636 1.37 0.00 39.29 6.636 524 ACCTTCCCTAATACAAAAGCTAAG 154 24 0 37.50 57.363 0.00 0.00 0.00 6.637 525 TGTACACACTGAACACGTTCCCTA 51 24 0 45.83 62.651 0.00 0.00 39.29 6.651 526 CCTAAGAATGTGGCCAAGGGAATG 122 24 0 50.00 62.658 0.00 0.00 0.00 6.658 527 CTGAAGACCTTCCCTAATACAAAAG 148 25 0 40.00 58.320 7.76 0.00 39.29 6.680 528 TACACACTGAAGATGTTCCCTAAGAA 104 26 0 38.46 60.690 0.00 0.00 0.00 6.690 529 TGAAGACCTTCCCTAATACAAAAG 149 24 0 37.50 57.304 7.76 0.00 39.29 6.696 530 CTGAAGACCTTCCCTAATACAAAA 148 24 0 37.50 57.304 7.76 0.00 39.29 6.696 531 AAGACCTTCCCTAATACAAAAGCTAA 151 26 0 34.62 59.266 0.00 0.00 0.00 6.734 532 CATGTACACACTGAAGATGTTCCCT 100 25 0 44.00 61.749 0.00 0.00 0.00 6.749 533 CAAGGGAATGCACTGAAGACCTTC 136 24 0 50.00 62.809 3.48 3.48 40.16 6.809 534 GAATGCACTGAAGACCTTCCCTAAT 141 25 0 44.00 61.816 0.24 0.00 39.29 6.816 535 AGATGTTCCCTAAGAATGTGGCCA 114 24 0 45.83 62.833 7.04 3.46 37.34 6.833 536 CACTGAAGATGTTCCCTAAGAATGTG 108 26 0 42.31 60.846 0.00 0.00 37.34 6.846 537 CACACTGAAGATGTTCCCTAAGAATG 106 26 0 42.31 60.846 0.00 0.00 37.34 6.846 538 CACTGAAGACTTCCCTACATACAGC 10 25 0 48.00 61.846 4.66 0.00 0.00 6.846 539 CTGAAGACCTTCCCTAATACAAAAGC 148 26 0 42.31 60.848 7.76 0.00 39.29 6.848 540 GAAGACTTCCCTACATACAGCCAAG 14 25 0 48.00 61.855 4.25 0.00 0.00 6.855 541 CCTTCCCTAATACAAAAGCTAAGA 155 24 0 37.50 57.117 0.00 0.00 0.00 6.883 542 ACACACTGAACACGTTCCCTAAGA 54 24 0 45.83 62.898 0.00 0.00 39.29 6.898 543 ATCACACTCACTGAAGACTTCCCTA 2 25 0 44.00 61.950 0.00 0.00 0.00 6.950 544 ATGTACACACTGAAGATGTTCCCTAA 101 26 0 38.46 60.974 0.00 0.00 0.00 6.974 545 ATACAAAAGCTAAGACATTGTGGGTG 164 26 0 38.46 61.018 8.27 0.00 0.00 7.018 546 TGAAGATGTTCCCTAAGAATGTGGC 111 25 0 44.00 62.045 0.00 0.00 37.34 7.045 547 CCCTAAGAAATGTGGTTAAGGGACC 70 25 0 48.00 62.052 0.00 0.00 40.50 7.052 548 AACACGTTCCCTAAGAAATGTGGTT 62 25 0 40.00 62.106 8.91 2.23 43.64 7.106 549 TAATACAAAAGCTAAGACATTGTGGG 162 26 0 34.62 58.892 8.27 0.00 0.00 7.108 550 AAGACCTTCCCTAATACAAAAGCTAAG 151 27 0 37.04 60.127 0.00 0.00 0.00 7.127 551 GAAGACCTTCCCTAATACAAAAGCTAA 150 27 0 37.04 60.127 0.00 0.00 34.16 7.127 552 TCCCTAATACAAAAGCTAAGACATTGT 158 27 0 33.33 59.853 2.37 2.37 0.00 7.147 553 CCTTCCCTAATACAAAAGCTAAGACAT 155 27 0 37.04 60.181 0.00 0.00 0.00 7.181 554 ACACTCACTGAAGACTTCCCTACAT 5 25 0 44.00 62.185 0.00 0.00 0.00 7.185 555 ACACTGAAGATGTTCCCTAAGAATGT 107 26 0 38.46 61.203 0.00 0.00 37.34 7.203 556 ACACACTGAAGATGTTCCCTAAGAAT 105 26 0 38.46 61.203 0.00 0.00 37.34 7.203 557 TGAAGACCTTCCCTAATACAAAAGCT 149 26 0 38.46 61.206 7.76 0.00 39.29 7.206 558 AAAGCTAAGACATTGTGGGTGTACC 169 25 0 44.00 62.214 0.00 0.00 40.81 7.214 559 CTTCCCTAATACAAAAGCTAAGACA 156 25 0 36.00 57.783 0.00 0.00 0.00 7.217 560 GTACACACTGAAGATGTTCCCTAAGA 103 26 0 42.31 61.245 0.00 0.00 0.00 7.245 561 CTAATACAAAAGCTAAGACATTGTGGG 161 27 0 37.04 59.754 0.00 0.00 0.00 7.246 562 CCTAATACAAAAGCTAAGACATTGTGG 160 27 0 37.04 59.754 3.29 0.00 0.00 7.246 563 CCCTAATACAAAAGCTAAGACATTGTG 159 27 0 37.04 59.754 8.27 0.00 0.00 7.246 564 CACGTTCCCTAAGAAATGTGGTTAAG 64 26 0 42.31 61.282 8.20 0.00 39.25 7.282 565 TAATACAAAAGCTAAGACATTGTGGGT 162 27 0 33.33 60.290 3.86 0.00 0.00 7.290 566 CCTAAGAAATGTGGTTAAGGGACCG 71 25 0 48.00 62.317 0.00 0.00 43.40 7.317 567 CCCTAATACAAAAGCTAAGACATTGT 159 26 0 34.62 58.663 2.37 2.37 0.00 7.337 568 ACAAAAGCTAAGACATTGTGGGTGT 166 25 0 40.00 62.343 0.17 0.00 0.00 7.343 569 CTCACTGAAGACTTCCCTACATACAG 8 26 0 46.15 61.349 4.00 0.00 0.00 7.349 570 CACACTGAACACGTTCCCTAAGAAA 55 25 0 44.00 62.359 1.37 0.00 39.29 7.359 571 TGAACACGTTCCCTAAGAAATGTGG 60 25 0 44.00 62.429 16.45 4.35 43.64 7.429 572 GAACACGTTCCCTAAGAAATGTGGT 61 25 0 44.00 62.430 16.45 12.23 43.45 7.430 573 GTACACACTGAACACGTTCCCTAAG 52 25 0 48.00 62.449 0.00 0.00 39.29 7.449 574 TGTACACACTGAAGATGTTCCCTAAG 102 26 0 42.31 61.468 0.00 0.00 0.00 7.468 575 TGCACTGAAGACCTTCCCTAATACA 144 25 0 44.00 62.481 0.24 0.00 39.29 7.481 576 TGAAGACTTCCCTACATACAGCCAA 13 25 0 44.00 62.481 13.23 0.00 0.00 7.481 577 CATGTACACACTGAAGATGTTCCCTA 100 26 0 42.31 61.524 0.00 0.00 0.00 7.524 578 CCATGTACACACTGAAGATGTTCCC 99 25 0 48.00 62.539 0.00 0.00 0.00 7.539 579 TCCCTAATACAAAAGCTAAGACATTG 158 26 0 34.62 58.438 0.00 0.00 0.00 7.562 580 CACTCACTGAAGACTTCCCTACATAC 6 26 0 46.15 61.568 0.00 0.00 0.00 7.568 581 GAATGCACTGAAGACCTTCCCTAATA 141 26 0 42.31 61.588 0.00 0.00 39.29 7.588 582 GAAGATGTTCCCTAAGAATGTGGCC 112 25 0 48.00 62.611 0.00 0.00 37.34 7.611 583 TACACACTGAACACGTTCCCTAAGA 53 25 0 44.00 62.621 0.00 0.00 39.29 7.621 584 GACCTTCCCTAATACAAAAGCTAAGAC 153 27 0 40.74 60.665 0.00 0.00 0.00 7.665 585 TCACACTCACTGAAGACTTCCCTAC 3 25 0 48.00 62.678 13.23 0.00 0.00 7.678 586 TTCCCTAAGAAATGTGGTTAAGGGAC 68 26 0 42.31 61.711 0.00 0.00 36.68 7.711 587 GTTCCCTAAGAAATGTGGTTAAGGGA 67 26 0 42.31 61.711 0.00 0.00 36.55 7.711 588 CAAAAGCTAAGACATTGTGGGTGTAC 167 26 0 42.31 61.721 0.00 0.00 0.00 7.721 589 ACTGAAGATGTTCCCTAAGAATGTGG 109 26 0 42.31 61.759 0.00 0.00 37.34 7.759 590 TCCCTAATACAAAAGCTAAGACATT 158 25 0 32.00 57.218 0.00 0.00 0.00 7.782 591 TTCCCTAATACAAAAGCTAAGACAT 157 25 0 32.00 57.218 0.00 0.00 0.00 7.782 592 AGACCTTCCCTAATACAAAAGCTAAGA 152 27 0 37.04 60.787 0.00 0.00 0.00 7.787 593 CTTCCCTAATACAAAAGCTAAGACAT 156 26 0 34.62 58.208 0.00 0.00 0.00 7.792 594 CTGAAGACTTCCCTACATACAGCCA 12 25 0 48.00 62.809 13.23 0.00 0.00 7.809 595 ACTGAAGACTTCCCTACATACAGCC 11 25 0 48.00 62.810 4.66 0.00 0.00 7.810 596 AATGCACTGAAGACCTTCCCTAATAC 142 26 0 42.31 61.813 0.00 0.00 39.29 7.813 597 CCCTAATACAAAAGCTAAGACATTG 159 25 0 36.00 57.176 0.00 0.00 0.00 7.824 598 CACTGAAGACCTTCCCTAATACAAAAG 146 27 0 40.74 60.826 0.00 0.00 39.29 7.826 599 GATGTTCCCTAAGAATGTGGCCAAG 115 25 0 48.00 62.840 0.00 0.00 37.34 7.840 600 ACACGTTCCCTAAGAAATGTGGTTAA 63 26 0 38.46 61.868 16.45 0.00 43.64 7.868 601 AACACGTTCCCTAAGAAATGTGGTTA 62 26 0 38.46 61.868 10.14 0.00 43.64 7.868 602 CACACTCACTGAAGACTTCCCTACA 4 25 0 48.00 62.908 13.23 0.00 0.00 7.908 603 ACTCACTGAAGACTTCCCTACATACA 7 26 0 42.31 61.941 0.00 0.00 0.00 7.941 604 ACACTCACTGAAGACTTCCCTACATA 5 26 0 42.31 61.941 0.00 0.00 0.00 7.941 605 GATCACACTCACTGAAGACTTCCCT 1 25 0 48.00 62.974 0.00 0.00 0.00 7.974 606 GCACTGAAGACCTTCCCTAATACAAA 145 26 0 42.31 61.976 0.00 0.00 39.29 7.976 607 TACACACTGAAGATGTTCCCTAAGAAT 104 27 0 37.04 61.003 0.00 0.00 37.34 8.003 608 TGAAGACCTTCCCTAATACAAAAGCTA 149 27 0 37.04 61.005 7.76 0.00 39.29 8.005 609 TTCCCTAATACAAAAGCTAAGACATTG 157 27 0 33.33 58.991 0.00 0.00 0.00 8.009 610 ACAAAAGCTAAGACATTGTGGGTGTA 166 26 0 38.46 62.095 0.17 0.00 0.00 8.095 611 TACAAAAGCTAAGACATTGTGGGTGT 165 26 0 38.46 62.095 8.27 0.00 0.00 8.095 612 TTCCCTAATACAAAAGCTAAGACATT 157 26 0 30.77 57.847 0.00 0.00 0.00 8.153 613 ACGTTCCCTAAGAAATGTGGTTAAGG 65 26 0 42.31 62.196 0.00 0.00 36.55 8.196 614 CTAATACAAAAGCTAAGACATTGTGG 161 26 0 34.62 57.804 0.00 0.00 0.00 8.196 615 CCTAATACAAAAGCTAAGACATTGTG 160 26 0 34.62 57.804 8.27 0.00 0.00 8.196 616 ACCTTCCCTAATACAAAAGCTAAGACA 154 27 0 37.04 61.225 0.00 0.00 0.00 8.225 617 CTTCCCTAATACAAAAGCTAAGACATT 156 27 0 33.33 58.771 0.00 0.00 0.00 8.229 618 AATACAAAAGCTAAGACATTGTGGGTG 163 27 0 37.04 61.473 8.27 0.00 0.00 8.473 619 ACTGAACACGTTCCCTAAGAAATGTG 58 26 0 42.31 62.617 12.57 12.57 44.20 8.617 620 CACTGAACACGTTCCCTAAGAAATGT 57 26 0 42.31 62.617 0.00 0.00 38.27 8.617 621 ACACTGAACACGTTCCCTAAGAAATG 56 26 0 42.31 62.617 0.00 0.00 39.29 8.617 622 CACACTGAACACGTTCCCTAAGAAAT 55 26 0 42.31 62.617 1.37 0.00 39.29 8.617 623 AAAAGCTAAGACATTGTGGGTGTACC 168 26 0 42.31 62.641 0.00 0.00 40.81 8.641 624 GTACACACTGAAGATGTTCCCTAAGAA 103 27 0 40.74 61.693 0.00 0.00 0.00 8.693 625 GATCACACTCACTGAAGACTTCCCTA 1 26 0 46.15 62.704 0.00 0.00 0.00 8.704 626 CGTTCCCTAAGAAATGTGGTTAAGGG 66 26 0 46.15 62.737 0.00 0.00 36.55 8.737 627 ATGCACTGAAGACCTTCCCTAATACA 143 26 0 42.31 62.741 0.24 0.00 39.29 8.741 628 ATGTACACACTGAAGATGTTCCCTAAG 101 27 0 40.74 61.750 0.00 0.00 0.00 8.750 629 CTGAAGATGTTCCCTAAGAATGTGGC 110 26 0 46.15 62.791 0.00 0.00 37.34 8.791 630 GAACACGTTCCCTAAGAAATGTGGTT 61 26 0 42.31 62.845 10.59 5.44 41.97 8.845 631 TACAAAAGCTAAGACATTGTGGGTGTA 165 27 0 37.04 61.865 8.27 0.00 0.00 8.865 632 TGCACTGAAGACCTTCCCTAATACAA 144 26 0 42.31 62.904 0.24 0.00 39.29 8.904 633 ATCACACTCACTGAAGACTTCCCTAC 2 26 0 46.15 62.927 13.23 0.00 0.00 8.927 634 CATGTACACACTGAAGATGTTCCCTAA 100 27 0 40.74 61.961 0.00 0.00 0.00 8.961 635 CTGAAGACCTTCCCTAATACAAAAGCT 148 27 0 40.74 61.974 7.76 0.00 39.29 8.974 636 TCACTGAAGACTTCCCTACATACAGC 9 26 0 46.15 62.978 4.66 0.00 0.00 8.978 637 ACACTGAAGATGTTCCCTAAGAATGTG 107 27 0 40.74 62.180 0.40 0.40 37.34 9.180 638 CACACTGAAGATGTTCCCTAAGAATGT 106 27 0 40.74 62.180 0.00 0.00 37.34 9.180 639 ACACACTGAAGATGTTCCCTAAGAATG 105 27 0 40.74 62.180 0.00 0.00 37.34 9.180 640 ACTGAAGACCTTCCCTAATACAAAAGC 147 27 0 40.74 62.187 0.00 0.00 39.29 9.187 641 AACACGTTCCCTAAGAAATGTGGTTAA 62 27 0 37.04 62.294 10.14 0.00 43.64 9.294 642 ATACAAAAGCTAAGACATTGTGGGTGT 164 27 0 37.04 62.357 8.27 0.00 0.00 9.357 643 GCACTGAAGACCTTCCCTAATACAAAA 145 27 0 40.74 62.398 0.00 0.00 39.29 9.398 644 GAATGCACTGAAGACCTTCCCTAATAC 141 27 0 44.44 62.540 0.00 0.00 39.29 9.540 645 TGTACACACTGAAGATGTTCCCTAAGA 102 27 0 40.74 62.581 0.00 0.00 0.00 9.581 646 GAACACGTTCCCTAAGAAATGTGGTTA 61 27 0 40.74 62.592 16.45 0.00 41.97 9.592 647 ACACGTTCCCTAAGAAATGTGGTTAAG 63 27 0 40.74 62.597 16.45 0.00 43.64 9.597 648 GTTCCCTAAGAAATGTGGTTAAGGGAC 67 27 0 44.44 62.664 0.00 0.00 36.68 9.664 649 ACTCACTGAAGACTTCCCTACATACAG 7 27 0 44.44 62.674 0.00 0.00 0.00 9.674 650 CACTGAAGATGTTCCCTAAGAATGTGG 108 27 0 44.44 62.703 0.00 0.00 37.34 9.703 651 CACTCACTGAAGACTTCCCTACATACA 6 27 0 44.44 62.883 0.00 0.00 0.00 9.883 652 CACACTCACTGAAGACTTCCCTACATA 4 27 0 44.44 62.883 0.00 0.00 0.00 9.883 653 ACACTCACTGAAGACTTCCCTACATAC 5 27 0 44.44 62.884 0.00 0.00 0.00 9.884 primer3-2.3.6/test/primer1_th_list_last/SP01-03.txt.rev0000664000077300007730000001424312231156243021426 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 GTCACCAAATGCACCCAGAC 103 20 0 55.00 60.312 0.00 0.00 0.00 0.312 1 GAAGCAAATGGACGTGGCAT 166 20 0 50.00 60.383 0.00 0.00 0.00 0.383 2 AAGCAAATGGACGTGGCATC 165 20 0 50.00 60.383 0.00 0.00 0.00 0.383 3 GTGTCACCAAATGCACCCAG 105 20 0 55.00 60.595 0.00 0.00 33.06 0.595 4 CACCAAATGCACCCAGACAC 101 20 0 55.00 60.595 0.00 0.00 0.00 0.595 5 TGTCACCAAATGCACCCAGA 104 20 0 50.00 60.753 0.00 0.00 0.00 0.753 6 TCACCAAATGCACCCAGACA 102 20 0 50.00 60.753 0.00 0.00 0.00 0.753 7 GCTGAAGCAAATGGACGTGG 169 20 0 55.00 61.007 0.00 0.00 41.90 1.007 8 CTGAAGCAAATGGACGTGGC 168 20 0 55.00 61.007 0.00 0.00 0.00 1.007 9 TGAAGCAAATGGACGTGGC 167 19 0 52.63 59.946 0.00 0.00 0.00 1.054 10 GAAGCAAATGGACGTGGCA 166 19 0 52.63 59.946 0.00 0.00 0.00 1.054 11 GGTGTCACCAAATGCACCC 106 19 0 57.89 60.243 18.20 0.00 44.57 1.243 12 AGCAAATGGACGTGGCATC 164 19 0 52.63 59.721 0.00 0.00 0.00 1.279 13 CCTGCGGTGTCACCAAATG 111 19 0 57.89 60.316 4.37 0.00 39.24 1.316 14 CAAATGGACGTGGCATCCG 162 19 0 57.89 60.461 3.53 0.00 39.91 1.461 15 GTGTCACCAAATGCACCCA 105 19 0 52.63 59.490 0.00 0.00 33.06 1.510 16 CGGTGTCACCAAATGCACC 107 19 0 57.89 60.609 22.47 9.83 45.61 1.609 17 AAGCAAATGGACGTGGCAT 165 19 0 47.37 59.260 0.00 0.00 0.00 1.740 18 TGAAGCAAATGGACGTGGCA 167 20 0 50.00 61.744 0.00 0.00 0.00 1.744 19 GCAAATGGACGTGGCATCC 163 19 0 57.89 60.756 0.69 0.69 36.99 1.756 20 ACCAAATGCACCCAGACACC 100 20 0 55.00 61.760 0.00 0.00 0.00 1.760 21 TGTCACCAAATGCACCCAG 104 19 0 52.63 59.185 0.00 0.00 0.00 1.815 22 CACCAAATGCACCCAGACA 101 19 0 52.63 59.185 0.00 0.00 0.00 1.815 23 ACCAAATGCACCCAGACAC 100 19 0 52.63 59.185 0.00 0.00 0.00 1.815 24 CTGCGGTGTCACCAAATGC 110 19 0 57.89 60.971 16.94 12.21 39.24 1.971 25 GGTGTCACCAAATGCACCCA 106 20 0 55.00 62.042 18.20 0.00 44.57 2.042 26 GTCACCAAATGCACCCAGA 103 19 0 52.63 58.882 0.00 0.00 0.00 2.118 27 TCACCAAATGCACCCAGAC 102 19 0 52.63 58.882 0.00 0.00 0.00 2.118 28 TGCGGTGTCACCAAATGC 109 18 0 55.56 59.855 16.94 12.21 39.24 2.145 29 GCGGTGTCACCAAATGCA 108 18 0 55.56 59.855 17.73 0.00 39.24 2.145 30 AGCAAATGGACGTGGCATCC 164 20 0 55.00 62.235 0.69 0.69 36.99 2.235 31 GCGGTGTCACCAAATGCAC 108 19 0 57.89 61.259 17.73 0.00 39.24 2.259 32 GAAGCAAATGGACGTGGCATC 166 21 0 52.38 61.372 0.00 0.00 0.00 2.372 33 GCTGAAGCAAATGGACGTG 169 19 0 52.63 58.492 0.00 0.00 41.90 2.508 34 CTGCGGTGTCACCAAATGCA 110 20 0 55.00 62.705 20.81 19.12 39.24 2.705 35 TGCGGTGTCACCAAATGCA 109 19 0 52.63 61.741 20.81 18.16 39.24 2.741 36 TGCGGTGTCACCAAATGCAC 109 20 0 55.00 62.978 20.81 4.05 39.24 2.978 37 AAATGGACGTGGCATCCG 161 18 0 55.56 58.992 3.53 0.00 39.91 3.008 38 GTGTCACCAAATGCACCCAGA 105 21 0 52.38 62.021 0.00 0.00 33.06 3.021 39 TGTCACCAAATGCACCCAGAC 104 21 0 52.38 62.021 1.08 1.08 0.00 3.021 40 GTCACCAAATGCACCCAGACA 103 21 0 52.38 62.021 2.79 0.00 0.00 3.021 41 TCACCAAATGCACCCAGACAC 102 21 0 52.38 62.021 0.00 0.00 0.00 3.021 42 TGAAGCAAATGGACGTGGCAT 167 21 0 47.62 62.089 0.00 0.00 0.00 3.089 43 CCTGCGGTGTCACCAAAT 111 18 0 55.56 58.824 22.47 0.00 39.24 3.176 44 GCCTGCGGTGTCACCAAAT 112 19 0 57.89 62.180 22.47 0.00 39.24 3.180 45 CTGAAGCAAATGGACGTGG 168 19 0 52.63 57.813 0.00 0.00 0.00 3.187 46 AAGCAAATGGACGTGGCA 165 18 0 50.00 58.736 0.00 0.00 0.00 3.264 47 AAATGGACGTGGCATCCGC 161 19 0 57.89 62.311 0.00 0.00 39.91 3.311 48 AGCAAATGGACGTGGCAT 164 18 0 50.00 58.490 0.00 0.00 0.00 3.510 49 CTCTGCCTGCGGTGTCAC 116 18 0 66.67 61.628 0.00 0.00 0.00 3.628 50 CTGAAGCAAATGGACGTGGCA 168 21 0 52.38 62.667 0.00 0.00 0.00 3.667 51 AATGGACGTGGCATCCGC 160 18 0 61.11 61.723 0.00 0.00 39.91 3.723 52 AAGCAAATGGACGTGGCATCC 165 21 0 52.38 62.752 0.69 0.69 36.99 3.752 53 GCCTGCGGTGTCACCAAA 112 18 0 61.11 61.808 22.47 8.65 39.24 3.808 54 GCAAATGGACGTGGCATC 163 18 0 55.56 58.087 0.00 0.00 0.00 3.913 55 GGTGTCACCAAATGCACCCAG 106 21 0 57.14 62.953 18.20 0.00 44.57 3.953 56 CACCAAATGCACCCAGACACC 101 21 0 57.14 62.953 0.00 0.00 0.00 3.953 57 GAAGCAAATGGACGTGGC 166 18 0 55.56 58.010 0.00 0.00 0.00 3.990 58 CGGTGTCACCAAATGCAC 107 18 0 55.56 57.936 22.47 0.00 39.24 4.064 59 TGTCACCAAATGCACCCA 104 18 0 50.00 57.903 0.00 0.00 0.00 4.097 60 CTGCGGTGTCACCAAATG 110 18 0 55.56 57.620 22.47 7.16 39.24 4.380 61 ACCAAATGCACCCAGACA 100 18 0 50.00 57.569 0.00 0.00 0.00 4.431 62 GGTGTCACCAAATGCACC 106 18 0 55.56 57.506 18.20 5.90 44.15 4.494 63 GTGTCACCAAATGCACCC 105 18 0 55.56 57.506 0.00 0.00 33.06 4.494 64 CAAATGGACGTGGCATCC 162 18 0 55.56 57.365 0.69 0.69 36.99 4.635 65 TCACCAAATGCACCCAGA 102 18 0 50.00 57.243 0.00 0.00 0.00 4.757 66 GTCACCAAATGCACCCAG 103 18 0 55.56 57.183 0.00 0.00 0.00 4.817 67 CACCAAATGCACCCAGAC 101 18 0 55.56 57.183 0.00 0.00 0.00 4.817 68 CCTCTGCCTGCGGTGTCA 117 18 0 66.67 62.919 0.00 0.00 0.00 4.919 69 TGAAGCAAATGGACGTGGCATC 167 22 0 50.00 62.949 0.00 0.00 0.00 4.949 70 CTGAAGCAAATGGACGTGGCAT 168 22 0 50.00 62.956 0.00 0.00 0.00 4.956 primer3-2.3.6/test/primer1_th_list_last/included_test_w_middle.rev0000664000077300007730000011263312231156243024401 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 ACACACACACACAACACACA 179 20 0 45.00 59.314 0.00 0.00 0.00 0.686 1 ACACACACACAACACACAGT 177 20 0 45.00 59.025 0.00 0.00 0.00 0.975 2 ACACAACAGACACACACACAC 188 21 0 47.62 60.105 0.00 0.00 0.00 1.105 3 ACAGACACACACACACAACAC 183 21 0 47.62 60.105 0.00 0.00 0.00 1.105 4 AGACACACACACACAACACAC 181 21 0 47.62 60.105 0.00 0.00 0.00 1.105 5 ACACACACACAACACACAGTC 177 21 0 47.62 60.105 0.00 0.00 0.00 1.105 6 CACAACAGACACACACACACA 187 21 0 47.62 60.105 0.00 0.00 0.00 1.105 7 CAGACACACACACACAACACA 182 21 0 47.62 60.105 0.00 0.00 0.00 1.105 8 CACACACACAACACACAGTCA 176 21 0 47.62 60.105 0.00 0.00 0.00 1.105 9 ACACAACAGACACACACACA 188 20 0 45.00 58.738 0.00 0.00 0.00 1.262 10 ACAACAGACACACACACACA 186 20 0 45.00 58.738 0.00 0.00 0.00 1.262 11 ACAGACACACACACACAACA 183 20 0 45.00 58.738 0.00 0.00 0.00 1.262 12 AGACACACACACACAACACA 181 20 0 45.00 58.738 0.00 0.00 0.00 1.262 13 ACACACACAACACACAGTCA 175 20 0 45.00 58.738 0.00 0.00 0.00 1.262 14 GACACACACACACAACACAC 180 20 0 50.00 58.645 0.00 0.00 0.00 1.355 15 CACACACACACAACACACAG 178 20 0 50.00 58.642 0.00 0.00 0.00 1.358 16 GACACACACACACAACACACA 180 21 0 47.62 60.374 0.00 0.00 0.00 1.374 17 ACACACACACACAACACACAG 179 21 0 47.62 60.374 0.00 0.00 0.00 1.374 18 CACACACACACAACACACAGT 178 21 0 47.62 60.374 0.00 0.00 0.00 1.374 19 ACAACAGACACACACACACAA 186 21 0 42.86 59.421 0.00 0.00 0.00 1.579 20 AACAGACACACACACACAACA 184 21 0 42.86 59.421 0.00 0.00 0.00 1.579 21 ACACACACAACACACAGTCAA 175 21 0 42.86 59.421 0.00 0.00 0.00 1.579 22 CACAACAGACACACACACAC 187 20 0 50.00 58.362 0.00 0.00 0.00 1.638 23 CAGACACACACACACAACAC 182 20 0 50.00 58.362 0.00 0.00 0.00 1.638 24 CACACACACAACACACAGTC 176 20 0 50.00 58.362 0.00 0.00 0.00 1.638 25 CAACAGACACACACACACAAC 185 21 0 47.62 59.044 0.00 0.00 0.00 1.956 26 ACAACACACAGTCAAGAAGCT 169 21 0 42.86 58.935 0.00 0.00 0.00 2.065 27 TACACAACAGACACACACACAC 189 22 0 45.45 59.916 0.00 0.00 0.00 2.084 28 ACACACAACACACAGTCAAGA 173 21 0 42.86 58.870 0.00 0.00 0.00 2.130 29 CACAACACACAGTCAAGAAGC 170 21 0 47.62 58.840 0.00 0.00 0.00 2.160 30 CACACACAACACACAGTCAAGA 174 22 0 45.45 60.174 0.00 0.00 0.00 2.174 31 CACACACAACACACAGTCAAG 174 21 0 47.62 58.774 0.00 0.00 0.00 2.226 32 CACAACACACAGTCAAGAAGCT 170 22 0 45.45 60.239 0.00 0.00 0.00 2.239 33 CAACAGACACACACACACAA 185 20 0 45.00 57.629 0.00 0.00 0.00 2.371 34 CACACACAACACACAGTCAA 174 20 0 45.00 57.629 0.00 0.00 0.00 2.371 35 AACAGACACACACACACAAC 184 20 0 45.00 57.627 0.00 0.00 0.00 2.373 36 TACACAACAGACACACACACA 189 21 0 42.86 58.599 0.00 0.00 0.00 2.401 37 ACACACACAACACACAGTCAAG 175 22 0 45.45 60.432 0.00 0.00 0.00 2.432 38 ACACACAACACACAGTCAAGAA 173 22 0 40.91 59.519 0.00 0.00 0.00 2.481 39 ACACAACACACAGTCAAGAAGC 171 22 0 45.45 60.497 0.00 0.00 0.00 2.497 40 ACAACACACAGTCAAGAAGC 169 20 0 45.00 57.405 0.00 0.00 0.00 2.595 41 ACACACAACACACAGTCAAG 173 20 0 45.00 57.337 0.00 0.00 0.00 2.663 42 ACACACACACACAACATACACT 243 22 0 40.91 59.322 0.00 0.00 0.00 2.678 43 CACAACAGACACACACACACAA 187 22 0 45.45 60.688 0.00 0.00 0.00 2.688 44 CAACAGACACACACACACAACA 185 22 0 45.45 60.688 0.00 0.00 0.00 2.688 45 CACACACACAACACACAGTCAA 176 22 0 45.45 60.688 0.00 0.00 0.00 2.688 46 ACAACAGACACACACACACAAC 186 22 0 45.45 60.689 0.00 0.00 0.00 2.689 47 AACAGACACACACACACAACAC 184 22 0 45.45 60.689 0.00 0.00 0.00 2.689 48 CAACACACAGTCAAGAAGCT 168 20 0 45.00 57.112 0.00 0.00 0.00 2.888 49 ACACACAGTCAAGAAGCTACA 166 21 0 42.86 58.106 0.00 0.00 0.00 2.894 50 ATACACAACAGACACACACACA 190 22 0 40.91 59.059 0.00 0.00 0.00 2.941 51 CACACAACACACAGTCAAGA 172 20 0 45.00 57.052 0.00 0.00 0.00 2.948 52 CACAGTCAAGAAGCTACATCCA 163 22 0 45.45 58.995 0.00 0.00 0.00 3.005 53 ACACAGTCAAGAAGCTACATCC 164 22 0 45.45 58.995 0.00 0.00 0.00 3.005 54 ACAACACACAGTCAAGAAGCTAC 169 23 0 43.48 60.052 0.00 0.00 0.00 3.052 55 CACACACACACAACATACACTCT 242 23 0 43.48 60.052 0.00 0.00 0.00 3.052 56 CACAACACACAGTCAAGAAGCTA 170 23 0 43.48 60.052 0.00 0.00 0.00 3.052 57 CAACACACAGTCAAGAAGCTACA 168 23 0 43.48 60.052 0.00 0.00 0.00 3.052 58 GCTACATCCATACACAACACACA 151 23 0 43.48 59.926 0.00 0.00 0.00 3.074 59 CACACAACACACAGTCAAGAAG 172 22 0 45.45 58.896 0.00 0.00 0.00 3.104 60 ACACACACACACAACATACAC 243 21 0 42.86 57.878 0.00 0.00 0.00 3.122 61 CACACAACACACAGTCAAGAA 172 21 0 42.86 57.806 0.00 0.00 0.00 3.194 62 ACACACACACAACATACACTCT 241 22 0 40.91 58.793 0.00 0.00 0.00 3.207 63 ACAACACACAGTCAAGAAGCTA 169 22 0 40.91 58.793 0.00 0.00 0.00 3.207 64 AACACACAGTCAAGAAGCTACA 167 22 0 40.91 58.793 0.00 0.00 0.00 3.207 65 CACACAGTCAAGAAGCTACATCC 165 23 0 47.83 60.238 0.00 0.00 0.00 3.238 66 CACACACACACAACATACACTC 242 22 0 45.45 58.706 0.00 0.00 0.00 3.294 67 ACACACACACACAACATACACTC 243 23 0 43.48 60.300 0.00 0.00 0.00 3.300 68 ATACACAACAGACACACACACAC 190 23 0 43.48 60.300 0.00 0.00 0.00 3.300 69 CAGACACACACACACAACACAC 182 22 0 50.00 61.316 0.00 0.00 0.00 3.316 70 GACACACACACACAACACACAG 180 22 0 50.00 61.316 0.00 0.00 0.00 3.316 71 CACACACACACAACACACAGTC 178 22 0 50.00 61.316 0.00 0.00 0.00 3.316 72 AGCTACATCCATACACAACACAC 152 23 0 43.48 59.675 0.00 0.00 0.00 3.325 73 ACACACAGTCAAGAAGCTACATC 166 23 0 43.48 59.615 0.00 0.00 0.00 3.385 74 CACACACACACAACATACACT 242 21 0 42.86 57.600 0.00 0.00 0.00 3.400 75 ACAGTCAAGAAGCTACATCCA 162 21 0 42.86 57.597 0.00 0.00 0.00 3.403 76 ACACACAGTCAAGAAGCTACAT 166 22 0 40.91 58.590 0.00 0.00 0.00 3.410 77 TGCACAATACCACACATGTTT 122 21 0 38.10 57.554 0.00 0.00 0.00 3.446 78 ACATCCATACACAACACACATGT 148 23 0 39.13 59.542 0.00 0.00 42.88 3.458 79 ACACAACACACAGTCAAGAAG 171 21 0 42.86 57.527 0.00 0.00 0.00 3.473 80 ACACACAACACACAGTCAAGAAG 173 23 0 43.48 60.486 0.00 0.00 0.00 3.486 81 CAACACACAGTCAAGAAGCTAC 168 22 0 45.45 58.445 0.00 0.00 0.00 3.555 82 CACACACACACAACACACA 178 19 0 47.37 57.435 0.00 0.00 0.00 3.565 83 ACACACACACACAACACAC 179 19 0 47.37 57.434 0.00 0.00 0.00 3.566 84 CACAGTCAAGAAGCTACATCCAT 163 23 0 43.48 59.421 0.00 0.00 0.00 3.579 85 AGCTACATCCATACACAACACA 152 22 0 40.91 58.384 0.00 0.00 0.00 3.616 86 ACACAGTCAAGAAGCTACATCCA 164 23 0 43.48 60.620 0.00 0.00 0.00 3.620 87 ATGCACAATACCACACATGTT 123 21 0 38.10 57.337 0.00 0.00 0.00 3.663 88 ACACACACACAACATACACTC 241 21 0 42.86 57.327 0.00 0.00 0.00 3.673 89 ATACACAACAGACACACACAC 190 21 0 42.86 57.327 0.00 0.00 0.00 3.673 90 GCTACATCCATACACAACACAC 151 22 0 45.45 58.308 0.00 0.00 0.00 3.692 91 CACACACAACACACAGTCAAGAA 174 23 0 43.48 60.732 0.00 0.00 0.00 3.732 92 CACAGTCAAGAAGCTACATCC 163 21 0 47.62 57.263 0.00 0.00 0.00 3.737 93 ACACAACAGACACACACACACA 188 22 0 45.45 61.737 0.00 0.00 0.00 3.737 94 ACAGACACACACACACAACACA 183 22 0 45.45 61.737 0.00 0.00 0.00 3.737 95 AGACACACACACACAACACACA 181 22 0 45.45 61.737 0.00 0.00 0.00 3.737 96 ACACACACACAACACACAGTCA 177 22 0 45.45 61.737 0.00 0.00 0.00 3.737 97 AACACACAGTCAAGAAGCTACAT 167 23 0 39.13 59.228 0.00 0.00 0.00 3.772 98 TACACACACACACAACATACACT 244 23 0 39.13 59.170 0.00 0.00 0.00 3.830 99 GACACACACACACAACACA 180 19 0 47.37 57.137 0.00 0.00 0.00 3.863 100 ACACACACACAACACACAG 177 19 0 47.37 57.128 0.00 0.00 0.00 3.872 101 CACACACACAACACACAGT 176 19 0 47.37 57.128 0.00 0.00 0.00 3.872 102 ACAGTCAAGAAGCTACATCCAT 162 22 0 40.91 58.107 0.00 0.00 0.00 3.893 103 ATGCACAATACCACACATGTTT 123 22 0 36.36 58.061 0.00 0.00 0.00 3.939 104 GATCTACACACACACACAACA 248 21 0 42.86 57.055 0.00 0.00 0.00 3.945 105 CACACACACAACATACACTCT 240 21 0 42.86 57.047 0.00 0.00 0.00 3.953 106 CAACACACAGTCAAGAAGCTA 168 21 0 42.86 57.047 0.00 0.00 0.00 3.953 107 AACACACAGTCAAGAAGCTAC 167 21 0 42.86 57.045 0.00 0.00 0.00 3.955 108 AAGCTACATCCATACACAACACA 153 23 0 39.13 59.034 0.00 0.00 0.00 3.966 109 ACACACACACACAACACACAGT 179 22 0 45.45 61.997 0.00 0.00 0.00 3.997 110 GAAGCTACATCCATACACAACACA 154 24 0 41.67 59.997 0.00 0.00 0.00 4.003 111 CACACAGTCAAGAAGCTACATC 165 22 0 45.45 57.988 0.00 0.00 0.00 4.012 112 TCTACACACACACACAACATACA 246 23 0 39.13 58.919 0.00 0.00 0.00 4.081 113 TATACACAACAGACACACACACA 191 23 0 39.13 58.919 0.00 0.00 0.00 4.081 114 ACATCCATACACAACACACATG 148 22 0 40.91 57.887 0.00 0.00 0.00 4.113 115 CATCCATACACAACACACATGT 147 22 0 40.91 57.887 0.00 0.00 42.88 4.113 116 CTACACACACACACAACATACACT 245 24 0 41.67 60.118 0.00 0.00 0.00 4.118 117 TCTACACACACACACAACATACAC 246 24 0 41.67 60.118 0.00 0.00 0.00 4.118 118 TACACACACACACAACATACACTC 244 24 0 41.67 60.118 0.00 0.00 0.00 4.118 119 TATACACAACAGACACACACACAC 191 24 0 41.67 60.118 0.00 0.00 0.00 4.118 120 CACACACACACAACATACACTCTA 242 24 0 41.67 59.879 0.00 0.00 0.00 4.121 121 CTACACACACACACAACATACAC 245 23 0 43.48 58.829 0.00 0.00 0.00 4.171 122 ACACAACACACATGTATATGCACA 140 24 0 37.50 60.177 0.00 0.00 39.26 4.177 123 AACACACAGTCAAGAAGCTACATC 167 24 0 41.67 60.177 0.00 0.00 0.00 4.177 124 TACACACACACACAACATACAC 244 22 0 40.91 57.783 0.00 0.00 0.00 4.217 125 AAGCTACATCCATACACAACACAC 153 24 0 41.67 60.237 0.00 0.00 0.00 4.237 126 AGAAGCTACATCCATACACAACAC 155 24 0 41.67 59.756 0.00 0.00 0.00 4.244 127 GCTACATCCATACACAACACACAT 151 24 0 41.67 60.297 0.00 0.00 0.00 4.297 128 ACACACACACAACATACACTCTA 241 23 0 39.13 58.662 0.00 0.00 0.00 4.338 129 ACACACACACAACATACACTCTAG 241 24 0 41.67 59.639 0.00 0.00 0.00 4.361 130 CACAACACACATGTATATGCACA 139 23 0 39.13 58.624 0.00 0.00 39.26 4.376 131 ACACAACACACATGTATATGCAC 140 23 0 39.13 58.623 0.00 0.00 39.26 4.377 132 CAACACACAGTCAAGAAGCTACAT 168 24 0 41.67 60.416 0.00 0.00 0.00 4.416 133 GATCTACACACACACACAACAT 248 22 0 40.91 57.574 0.00 0.00 0.00 4.426 134 AGAAGCTACATCCATACACAACA 155 23 0 39.13 58.521 0.00 0.00 0.00 4.479 135 CTACACACACACACAACATACA 245 22 0 40.91 57.517 0.00 0.00 0.00 4.483 136 GTCAAGAAGCTACATCCATACACA 159 24 0 41.67 59.515 0.00 0.00 0.00 4.485 137 TACACAACAGACACACACACACA 189 23 0 43.48 61.487 0.00 0.00 0.00 4.487 138 TGCACAATACCACACATGTTTA 122 22 0 36.36 57.471 0.00 0.00 0.00 4.529 139 GAAGCTACATCCATACACAACAC 154 23 0 43.48 58.444 0.00 0.00 0.00 4.556 140 CACACACACAACACACAGTCAAG 176 23 0 47.83 61.577 0.00 0.00 0.00 4.577 141 TGCACAATACCACACATGTTTAAC 122 24 0 37.50 59.401 0.00 0.00 0.00 4.599 142 GCACAATACCACACATGTTTAACA 121 24 0 37.50 59.401 0.00 0.00 0.00 4.599 143 CACACACACAACATACACTCTAGA 240 24 0 41.67 59.399 0.00 0.00 0.00 4.601 144 CACACACAACATACACTCTAGACA 238 24 0 41.67 59.399 0.00 0.00 0.00 4.601 145 CACACAACATACACTCTAGACACA 236 24 0 41.67 59.399 0.00 0.00 0.00 4.601 146 ACACACACAACATACACTCTAGAC 239 24 0 41.67 59.399 0.00 0.00 0.00 4.601 147 ACACACAACATACACTCTAGACAC 237 24 0 41.67 59.399 0.00 0.00 0.00 4.601 148 TACATCCATACACAACACACATGT 149 24 0 37.50 59.387 0.00 0.00 42.88 4.613 149 ACATCCATACACAACACACATGTA 148 24 0 37.50 59.387 0.00 0.00 39.26 4.613 150 CACACAACACACAGTCAAGAAGC 172 23 0 47.83 61.639 0.00 0.00 0.00 4.639 151 AAGCTACATCCATACACAACAC 153 22 0 40.91 57.359 0.00 0.00 0.00 4.641 152 ATCTACACACACACACAACATACA 247 24 0 37.50 59.331 0.00 0.00 0.00 4.669 153 ATATACACAACAGACACACACACA 192 24 0 37.50 59.331 0.00 0.00 0.00 4.669 154 ACACAACACACATGTATATGCA 140 22 0 36.36 57.281 0.00 0.00 39.26 4.719 155 ACAACACACATGTATATGCACA 138 22 0 36.36 57.281 0.00 0.00 39.26 4.719 156 CACAGTCAAGAAGCTACATCCATA 163 24 0 41.67 59.271 0.00 0.00 0.00 4.729 157 CAGTCAAGAAGCTACATCCATACA 161 24 0 41.67 59.271 0.00 0.00 0.00 4.729 158 ACAGTCAAGAAGCTACATCCATAC 162 24 0 41.67 59.271 0.00 0.00 0.00 4.729 159 AGTCAAGAAGCTACATCCATACAC 160 24 0 41.67 59.271 0.00 0.00 0.00 4.729 160 TCAAGAAGCTACATCCATACACA 158 23 0 39.13 58.265 0.00 0.00 0.00 4.735 161 TATGCACAATACCACACATGTT 124 22 0 36.36 57.263 0.00 0.00 0.00 4.737 162 ACACACACAACACACAGTCAAGA 175 23 0 43.48 61.740 0.00 0.00 0.00 4.740 163 TCTACACACACACACAACATAC 246 22 0 40.91 57.255 0.00 0.00 0.00 4.745 164 TATACACAACAGACACACACAC 191 22 0 40.91 57.255 0.00 0.00 0.00 4.745 165 CACAACACACATGTATATGCACAA 139 24 0 37.50 59.225 0.00 0.00 39.26 4.775 166 ACACAACACACAGTCAAGAAGCT 171 23 0 43.48 61.808 0.00 0.00 0.00 4.808 167 CACAACAGACACACACACACAAC 187 23 0 47.83 61.817 0.00 0.00 0.00 4.817 168 CAACAGACACACACACACAACAC 185 23 0 47.83 61.817 0.00 0.00 0.00 4.817 169 TGCACAATACCACACATGTTTAA 122 23 0 34.78 58.157 0.00 0.00 0.00 4.843 170 ACACAACATACACTCTAGACACAG 235 24 0 41.67 59.157 0.00 0.00 0.00 4.843 171 ACACACACAACATACACTCTAGA 239 23 0 39.13 58.151 0.00 0.00 0.00 4.849 172 ACACACAACATACACTCTAGACA 237 23 0 39.13 58.151 0.00 0.00 0.00 4.849 173 ACACAACATACACTCTAGACACA 235 23 0 39.13 58.151 0.00 0.00 0.00 4.849 174 AAGAAGCTACATCCATACACAACA 156 24 0 37.50 59.140 0.00 0.00 0.00 4.860 175 GAAGCTACATCCATACACAACA 154 22 0 40.91 57.096 0.00 0.00 0.00 4.904 176 CACACACACAACATACACTCTAG 240 23 0 43.48 58.081 0.00 0.00 0.00 4.919 177 ATATGCACAATACCACACATGT 125 22 0 36.36 57.054 0.00 0.00 0.00 4.946 178 CTACATCCATACACAACACACA 150 22 0 40.91 57.033 0.00 0.00 0.00 4.967 179 ACACAGTCAAGAAGCTACATCCAT 164 24 0 41.67 60.971 0.00 0.00 0.00 4.971 180 ACAGTCAAGAAGCTACATCCATA 162 23 0 39.13 58.004 0.00 0.00 0.00 4.996 181 AGTCAAGAAGCTACATCCATACA 160 23 0 39.13 58.004 0.00 0.00 0.00 4.996 182 TACACAACACACATGTATATGCACA 141 25 0 36.00 60.005 0.00 0.00 39.26 5.005 183 ACAACACACATGTATATGCACAA 138 23 0 34.78 57.973 0.00 0.00 39.26 5.027 184 TATGCACAATACCACACATGTTT 124 23 0 34.78 57.961 0.00 0.00 0.00 5.039 185 ATGCACAATACCACACATGTTTA 123 23 0 34.78 57.961 0.00 0.00 0.00 5.039 186 GTCAAGAAGCTACATCCATACAC 159 23 0 43.48 57.941 0.00 0.00 0.00 5.059 187 GTCAAGAAGCTACATCCATACACAA 159 25 0 40.00 60.063 0.00 0.00 0.00 5.063 188 TCAAGAAGCTACATCCATACACAAC 158 25 0 40.00 60.063 0.00 0.00 0.00 5.063 189 TCAAGAAGCTACATCCATACACAA 158 24 0 37.50 58.896 0.00 0.00 0.00 5.104 190 GCACAATACCACACATGTTTAAC 121 23 0 39.13 57.839 0.00 0.00 0.00 5.161 191 CACACACAACATACACTCTAGAC 238 23 0 43.48 57.832 0.00 0.00 0.00 5.168 192 CACACAACATACACTCTAGACAC 236 23 0 43.48 57.832 0.00 0.00 0.00 5.168 193 ACACACATGTATATGCACAATACCA 135 25 0 36.00 59.828 15.76 7.60 38.02 5.172 194 ACACATGTATATGCACAATACCACA 133 25 0 36.00 59.828 15.76 4.59 38.43 5.172 195 ACATGTATATGCACAATACCACACA 131 25 0 36.00 59.828 15.76 4.01 38.43 5.172 196 TGTATATGCACAATACCACACATGT 128 25 0 36.00 59.828 15.76 0.00 0.00 5.172 197 CACAACACACAGTCAAGAAGCTAC 170 24 0 45.83 61.177 0.00 0.00 0.00 5.177 198 CTACATCCATACACAACACACATG 150 24 0 41.67 58.810 0.00 0.00 0.00 5.190 199 CAAGAAGCTACATCCATACACAAC 157 24 0 41.67 58.809 0.00 0.00 0.00 5.191 200 TACATCCATACACAACACACATG 149 23 0 39.13 57.795 0.00 0.00 0.00 5.205 201 CATCCATACACAACACACATGTA 147 23 0 39.13 57.795 0.00 0.00 39.26 5.205 202 AGCTACATCCATACACAACACACA 152 24 0 41.67 61.209 0.00 0.00 0.00 5.209 203 ATGCACAATACCACACATGTTTAAC 123 25 0 36.00 59.776 0.00 0.00 0.00 5.224 204 ACATCCATACACAACACACATGTAT 148 25 0 36.00 59.769 0.00 0.00 39.26 5.231 205 ACACAACAGACACACACACACAA 188 23 0 43.48 62.233 0.00 0.00 0.00 5.233 206 ACAACAGACACACACACACAACA 186 23 0 43.48 62.233 0.00 0.00 0.00 5.233 207 AACAGACACACACACACAACACA 184 23 0 43.48 62.233 0.00 0.00 0.00 5.233 208 ACACACACACAACACACAGTCAA 177 23 0 43.48 62.233 0.00 0.00 0.00 5.233 209 GATCTACACACACACACAACATACA 248 25 0 40.00 60.236 0.00 0.00 0.00 5.236 210 ATATGCACAATACCACACATGTT 125 23 0 34.78 57.763 0.00 0.00 0.00 5.237 211 GATCTACACACACACACAACATAC 248 24 0 41.67 58.761 0.00 0.00 0.00 5.239 212 ATCTACACACACACACAACATAC 247 23 0 39.13 57.745 0.00 0.00 0.00 5.255 213 ATATACACAACAGACACACACAC 192 23 0 39.13 57.745 0.00 0.00 0.00 5.255 214 GCACAATACCACACATGTTTAACAG 121 25 0 40.00 60.292 0.00 0.00 0.00 5.292 215 CACACAACATACACTCTAGACACAG 236 25 0 44.00 60.293 0.00 0.00 0.00 5.293 216 CAAGAAGCTACATCCATACACAACA 157 25 0 40.00 60.294 0.00 0.00 0.00 5.294 217 CTACATCCATACACAACACACATGT 150 25 0 40.00 60.294 0.00 0.00 42.88 5.294 218 AAGAAGCTACATCCATACACAACAC 156 25 0 40.00 60.294 0.00 0.00 0.00 5.294 219 CAGTCAAGAAGCTACATCCATAC 161 23 0 43.48 57.686 0.00 0.00 0.00 5.314 220 CACAACACACATGTATATGCACAAT 139 25 0 36.00 59.605 0.00 0.00 39.26 5.395 221 ATGCACAATACCACACATGTTTAA 123 24 0 33.33 58.600 0.00 0.00 0.00 5.400 222 CACAGTCAAGAAGCTACATCCATAC 163 25 0 44.00 60.408 0.00 0.00 0.00 5.408 223 CAGTCAAGAAGCTACATCCATACAC 161 25 0 44.00 60.408 0.00 0.00 0.00 5.408 224 CACAACATACACTCTAGACACAG 234 23 0 43.48 57.579 0.00 0.00 0.00 5.421 225 CAAGAAGCTACATCCATACACAA 157 23 0 39.13 57.536 0.00 0.00 0.00 5.464 226 CTACATCCATACACAACACACAT 150 23 0 39.13 57.536 0.00 0.00 0.00 5.464 227 AAGAAGCTACATCCATACACAAC 156 23 0 39.13 57.535 0.00 0.00 0.00 5.465 228 ATCTACACACACACACAACATACAC 247 25 0 40.00 60.466 0.00 0.00 0.00 5.466 229 ATATACACAACAGACACACACACAC 192 25 0 40.00 60.466 0.00 0.00 0.00 5.466 230 TACACAACACACATGTATATGCAC 141 24 0 37.50 58.507 0.00 0.00 39.26 5.493 231 GATCTACACACACACACAACATA 248 23 0 39.13 57.495 0.00 0.00 0.00 5.505 232 CCATACACAACACACATGTATATGC 144 25 0 40.00 59.494 2.84 0.00 39.26 5.506 233 CACACATGTATATGCACAATACCAC 134 25 0 40.00 59.494 15.76 0.00 38.43 5.506 234 CACATGTATATGCACAATACCACAC 132 25 0 40.00 59.494 15.76 0.00 38.43 5.506 235 CACACACACAACATACACTCTAGAC 240 25 0 44.00 60.520 0.00 0.00 0.00 5.520 236 CACACACAACATACACTCTAGACAC 238 25 0 44.00 60.520 0.00 0.00 0.00 5.520 237 ACACACACACACAACATACACTCT 243 24 0 41.67 61.564 0.00 0.00 0.00 5.564 238 ACACAACACACAGTCAAGAAGCTA 171 24 0 41.67 61.564 0.00 0.00 0.00 5.564 239 ACAACACACAGTCAAGAAGCTACA 169 24 0 41.67 61.564 0.00 0.00 0.00 5.564 240 ACAACACACATGTATATGCACAAT 138 24 0 33.33 58.422 0.00 0.00 39.26 5.578 241 AGACACACACACACAACACACAG 181 23 0 47.83 62.582 0.00 0.00 0.00 5.582 242 CACACACAACACACAGTCAAGAAG 174 24 0 45.83 61.586 0.00 0.00 0.00 5.586 243 ATATGCACAATACCACACATGTTT 125 24 0 33.33 58.412 0.00 0.00 0.00 5.588 244 CACACATGTATATGCACAATACCA 134 24 0 37.50 58.313 15.76 7.60 38.43 5.687 245 CACATGTATATGCACAATACCACA 132 24 0 37.50 58.313 15.76 4.59 38.43 5.687 246 CATGTATATGCACAATACCACACA 130 24 0 37.50 58.313 15.76 4.01 31.46 5.687 247 TGTATATGCACAATACCACACATG 128 24 0 37.50 58.313 15.76 0.00 0.00 5.687 248 ACACACATGTATATGCACAATACC 135 24 0 37.50 58.312 15.76 3.34 38.02 5.688 249 ACACATGTATATGCACAATACCAC 133 24 0 37.50 58.312 15.76 0.00 38.43 5.688 250 ACATGTATATGCACAATACCACAC 131 24 0 37.50 58.312 15.76 0.00 38.43 5.688 251 GTATATGCACAATACCACACATGT 127 24 0 37.50 58.312 9.75 0.00 0.00 5.688 252 ACACAACACACATGTATATGCACAA 140 25 0 36.00 60.700 0.00 0.00 39.26 5.700 253 CACACAGTCAAGAAGCTACATCCA 165 24 0 45.83 61.738 0.00 0.00 0.00 5.738 254 ACACACAGTCAAGAAGCTACATCC 166 24 0 45.83 61.739 0.00 0.00 0.00 5.739 255 CACACACACACAACATACACTCTAG 242 25 0 44.00 60.746 0.00 0.00 0.00 5.746 256 CATCCATACACAACACACATGTAT 147 24 0 37.50 58.249 0.00 0.00 39.26 5.751 257 TACATCCATACACAACACACATGTA 149 25 0 36.00 59.243 0.00 0.00 39.26 5.757 258 ACACAGTCAAGAAGCTACATCCATA 164 25 0 40.00 60.765 0.00 0.00 0.00 5.765 259 ACAGTCAAGAAGCTACATCCATACA 162 25 0 40.00 60.765 0.00 0.00 0.00 5.765 260 AGTCAAGAAGCTACATCCATACACA 160 25 0 40.00 60.765 0.00 0.00 0.00 5.765 261 TACACAACACACATGTATATGCA 141 23 0 34.78 57.213 0.00 0.00 39.26 5.787 262 ATACACAACAGACACACACACACA 190 24 0 41.67 61.800 0.00 0.00 0.00 5.800 263 TGTATATACACAACAGACACACACA 195 25 0 36.00 59.190 16.53 0.00 0.00 5.810 264 TATATACACAACAGACACACACACA 193 25 0 36.00 59.190 0.00 0.00 0.00 5.810 265 CAGACACACACACACAACACACA 182 23 0 47.83 62.820 0.00 0.00 0.00 5.820 266 CACACACACACAACACACAGTCA 178 23 0 47.83 62.820 0.00 0.00 0.00 5.820 267 ACAGACACACACACACAACACAC 183 23 0 47.83 62.822 0.00 0.00 0.00 5.822 268 GACACACACACACAACACACAGT 180 23 0 47.83 62.822 0.00 0.00 0.00 5.822 269 ACACACACACACAACACACAGTC 179 23 0 47.83 62.822 0.00 0.00 0.00 5.822 270 TGCACAATACCACACATGTTTAACA 122 25 0 36.00 60.875 0.00 0.00 0.00 5.875 271 ACACACACACAACATACACTCTAGA 241 25 0 40.00 60.877 0.00 0.00 0.00 5.877 272 ACACACACAACATACACTCTAGACA 239 25 0 40.00 60.877 0.00 0.00 0.00 5.877 273 ACACACAACATACACTCTAGACACA 237 25 0 40.00 60.877 0.00 0.00 0.00 5.877 274 CTACACACACACACAACATACACTC 245 25 0 44.00 60.972 0.00 0.00 0.00 5.972 275 TACACAACAGACACACACACACAA 189 24 0 41.67 61.974 0.00 0.00 0.00 5.974 276 CAACACACATGTATATGCACAATACC 137 26 0 38.46 60.015 15.76 3.34 39.26 6.015 277 CATACACAACACACATGTATATGCAC 143 26 0 38.46 60.016 0.00 0.00 39.26 6.016 278 CACAATACCACACATGTTTAACAGA 120 25 0 36.00 58.967 3.60 0.00 0.00 6.033 279 CACAATACCACACATGTTTAACAGAC 120 26 0 38.46 60.070 3.60 0.00 0.00 6.070 280 CATACACAACACACATGTATATGCA 143 25 0 36.00 58.914 0.00 0.00 39.26 6.086 281 ATACACAACACACATGTATATGCAC 142 25 0 36.00 58.914 0.00 0.00 39.26 6.086 282 GAAGCTACATCCATACACAACACAC 154 25 0 44.00 61.090 0.00 0.00 0.00 6.090 283 AACACACATGTATATGCACAATACC 136 25 0 36.00 58.907 15.76 3.34 39.26 6.093 284 GTATATGCACAATACCACACATGTT 127 25 0 36.00 58.907 9.75 0.00 0.00 6.093 285 CTACATCCATACACAACACACATGTA 150 26 0 38.46 60.124 0.00 0.00 39.26 6.124 286 GTATATACACAACAGACACACACAC 194 25 0 40.00 58.872 8.63 0.00 0.00 6.128 287 TATGCACAATACCACACATGTTTA 124 24 0 33.33 57.868 0.00 0.00 0.00 6.132 288 CATGTATATGCACAATACCACACATG 130 26 0 38.46 59.850 12.26 9.17 31.83 6.150 289 ACATGTATATGCACAATACCACACAT 131 26 0 34.62 60.180 15.76 5.82 38.43 6.180 290 ATGTATATGCACAATACCACACATGT 129 26 0 34.62 60.180 15.76 0.00 31.46 6.180 291 ACACACACAACACACAGTCAAGAA 175 24 0 41.67 62.217 0.00 0.00 0.00 6.217 292 AGAAGCTACATCCATACACAACACA 155 25 0 40.00 61.231 0.00 0.00 0.00 6.231 293 CAACACACAGTCAAGAAGCTACATC 168 25 0 44.00 61.257 0.00 0.00 0.00 6.257 294 CATGTATATGCACAATACCACACAT 130 25 0 36.00 58.730 15.76 5.82 31.46 6.270 295 ATGTATATGCACAATACCACACATG 129 25 0 36.00 58.730 15.76 0.00 31.46 6.270 296 TGTATATACACAACAGACACACACAC 195 26 0 38.46 60.291 16.53 0.00 0.00 6.291 297 GTATATACACAACAGACACACACACA 194 26 0 38.46 60.291 8.63 0.00 0.00 6.291 298 TATATACACAACAGACACACACACAC 193 26 0 38.46 60.291 0.00 0.00 0.00 6.291 299 ATACACAACACACATGTATATGCA 142 24 0 33.33 57.692 0.00 0.00 39.26 6.308 300 CACAATACCACACATGTTTAACAG 120 24 0 37.50 57.686 0.00 0.00 0.00 6.314 301 TATATGCACAATACCACACATGTT 126 24 0 33.33 57.678 0.00 0.00 0.00 6.322 302 TGTATATACACAACAGACACACAC 195 24 0 37.50 57.663 16.53 0.00 0.00 6.337 303 GTATATACACAACAGACACACACA 194 24 0 37.50 57.663 8.63 0.00 0.00 6.337 304 TATATACACAACAGACACACACAC 193 24 0 37.50 57.663 0.00 0.00 0.00 6.337 305 TCTACACACACACACAACATACACT 246 25 0 40.00 61.338 0.00 0.00 0.00 6.338 306 TACACACACACACAACATACACTCT 244 25 0 40.00 61.338 0.00 0.00 0.00 6.338 307 ACACACACACACAACATACACTCTA 243 25 0 40.00 61.338 0.00 0.00 0.00 6.338 308 ATACACAACACACATGTATATGCACA 142 26 0 34.62 60.348 0.00 0.00 39.26 6.348 309 AACACACATGTATATGCACAATACCA 136 26 0 34.62 60.349 15.76 7.60 39.26 6.349 310 TGTATATGCACAATACCACACATGTT 128 26 0 34.62 60.349 15.76 0.00 0.00 6.349 311 GCTACATCCATACACAACACACATG 151 25 0 44.00 61.374 0.00 0.00 0.00 6.374 312 TATGCACAATACCACACATGTTTAAC 124 26 0 34.62 59.626 0.00 0.00 0.00 6.374 313 TACATCCATACACAACACACATGTAT 149 26 0 34.62 59.616 0.00 0.00 39.26 6.384 314 ACATCCATACACAACACACATGTATA 148 26 0 34.62 59.616 0.00 0.00 39.26 6.384 315 TATGCACAATACCACACATGTTTAA 124 25 0 32.00 58.487 0.00 0.00 0.00 6.513 316 ATGTATATGCACAATACCACACAT 129 24 0 33.33 57.486 15.76 5.82 31.46 6.514 317 TACACAACACACATGTATATGCACAA 141 26 0 34.62 60.515 0.00 0.00 39.26 6.515 318 AGCTACATCCATACACAACACACAT 152 25 0 40.00 61.523 0.00 0.00 0.00 6.523 319 CACAACACACATGTATATGCACAATA 139 26 0 34.62 59.461 0.00 0.00 39.26 6.539 320 ACAACACACATGTATATGCACAATAC 138 26 0 34.62 59.461 12.84 12.84 39.26 6.539 321 GTATATGCACAATACCACACATGTTT 127 26 0 34.62 59.457 9.75 0.00 0.00 6.543 322 TATACACAACAGACACACACACACA 191 25 0 40.00 61.566 0.00 0.00 0.00 6.566 323 CATACACAACACACATGTATATGC 143 24 0 37.50 57.398 0.00 0.00 39.26 6.602 324 TCCATACACAACACACATGTATATGC 145 26 0 38.46 60.680 2.84 0.00 39.26 6.680 325 ACAACACACATGTATATGCACAATA 138 25 0 32.00 58.316 0.00 0.00 39.26 6.684 326 TATATGCACAATACCACACATGTTT 126 25 0 32.00 58.306 0.00 0.00 0.00 6.694 327 ATATGCACAATACCACACATGTTTA 125 25 0 32.00 58.306 0.00 0.00 0.00 6.694 328 AAGCTACATCCATACACAACACACA 153 25 0 40.00 61.694 0.00 0.00 0.00 6.694 329 CACACACACAACACACAGTCAAGA 176 24 0 45.83 62.783 0.00 0.00 0.00 6.783 330 CACACAACACACAGTCAAGAAGCT 172 24 0 45.83 62.850 0.00 0.00 0.00 6.850 331 CATCCATACACAACACACATGTATA 147 25 0 36.00 58.150 0.00 0.00 39.26 6.850 332 TCCATACACAACACACATGTATATG 145 25 0 36.00 58.150 1.80 1.80 39.26 6.850 333 ACAACACACAGTCAAGAAGCTACAT 169 25 0 40.00 61.863 0.00 0.00 0.00 6.863 334 CCATACACAACACACATGTATATGCA 144 26 0 38.46 60.902 2.84 0.00 39.26 6.902 335 CACACATGTATATGCACAATACCACA 134 26 0 38.46 60.902 15.76 4.59 38.43 6.902 336 CACATGTATATGCACAATACCACACA 132 26 0 38.46 60.902 15.76 4.01 38.43 6.902 337 ACACACATGTATATGCACAATACCAC 135 26 0 38.46 60.902 15.76 0.00 38.02 6.902 338 ACACATGTATATGCACAATACCACAC 133 26 0 38.46 60.902 15.76 0.00 38.43 6.902 339 CAACACACATGTATATGCACAATAC 137 25 0 36.00 58.019 12.84 12.84 39.26 6.981 340 ACACAACACACATGTATATGCACAAT 140 26 0 34.62 61.018 0.00 0.00 39.26 7.018 341 CACACAGTCAAGAAGCTACATCCAT 165 25 0 44.00 62.029 0.00 0.00 0.00 7.029 342 ATATGCACAATACCACACATGTTTAAC 125 27 0 33.33 59.967 0.00 0.00 0.00 7.033 343 ACATCCATACACAACACACATGTATAT 148 27 0 33.33 59.963 0.00 0.00 39.26 7.037 344 ATATGCACAATACCACACATGTTTAA 125 26 0 30.77 58.886 0.00 0.00 0.00 7.114 345 GTCAAGAAGCTACATCCATACACAAC 159 26 0 42.31 61.115 0.00 0.00 0.00 7.115 346 TACACACACACACAACATACACTCTA 244 26 0 38.46 61.130 0.00 0.00 0.00 7.130 347 ATGCACAATACCACACATGTTTAACA 123 26 0 34.62 61.187 0.00 0.00 0.00 7.187 348 AACACACAGTCAAGAAGCTACATCC 167 25 0 44.00 62.198 0.00 0.00 0.00 7.198 349 AGTCAAGAAGCTACATCCATACACAA 160 26 0 38.46 61.252 0.00 0.00 0.00 7.252 350 ATACACAACAGACACACACACACAA 190 25 0 40.00 62.257 0.00 0.00 0.00 7.257 351 GATCTACACACACACACAACATACAC 248 26 0 42.31 61.271 0.00 0.00 0.00 7.271 352 CAAGAAGCTACATCCATACACAACAC 157 26 0 42.31 61.334 0.00 0.00 0.00 7.334 353 CATCCATACACAACACACATGTATATG 147 27 0 37.04 59.649 1.80 1.80 39.26 7.351 354 CATCCATACACAACACACATGTATAT 147 26 0 34.62 58.558 0.00 0.00 39.26 7.442 355 ATCCATACACAACACACATGTATATG 146 26 0 34.62 58.558 1.80 1.80 39.26 7.442 356 CTACATCCATACACAACACACATGTAT 150 27 0 37.04 60.450 0.00 0.00 39.26 7.450 357 GCACAATACCACACATGTTTAACAGA 121 26 0 38.46 61.452 3.60 0.00 0.00 7.452 358 TCAAGAAGCTACATCCATACACAACA 158 26 0 38.46 61.474 0.00 0.00 0.00 7.474 359 CACAACACACATGTATATGCACAATAC 139 27 0 37.04 60.497 12.84 12.84 39.26 7.497 360 GACATAGAACACACGTGTATATACA 210 25 0 36.00 57.481 16.53 14.12 44.80 7.519 361 TACATCCATACACAACACACATGTATA 149 27 0 33.33 59.474 0.00 0.00 39.26 7.526 362 CACACAACACACAGTCAAGAAGCTA 172 25 0 44.00 62.579 0.00 0.00 0.00 7.579 363 CACAACACACAGTCAAGAAGCTACA 170 25 0 44.00 62.579 0.00 0.00 0.00 7.579 364 ACACAACACACAGTCAAGAAGCTAC 171 25 0 44.00 62.581 0.00 0.00 0.00 7.581 365 ATCTACACACACACACAACATACACT 247 26 0 38.46 61.634 0.00 0.00 0.00 7.634 366 ATCCATACACAACACACATGTATAT 146 25 0 32.00 57.351 0.00 0.00 39.26 7.649 367 ATGTATATGCACAATACCACACATGTT 129 27 0 33.33 60.670 15.76 0.00 31.46 7.670 368 TGCACAATACCACACATGTTTAACAG 122 26 0 38.46 61.672 0.00 0.00 0.00 7.672 369 ACACACAACATACACTCTAGACACAG 237 26 0 42.31 61.676 0.00 0.00 0.00 7.676 370 GTATATGCACAATACCACACATGTTTA 127 27 0 33.33 59.323 9.75 0.00 0.00 7.677 371 AAGAAGCTACATCCATACACAACACA 156 26 0 38.46 61.698 0.00 0.00 0.00 7.698 372 TATATGCACAATACCACACATGTTTA 126 26 0 30.77 58.208 0.00 0.00 0.00 7.792 373 CACACAGTCAAGAAGCTACATCCATA 165 26 0 42.31 61.796 0.00 0.00 0.00 7.796 374 CACAGTCAAGAAGCTACATCCATACA 163 26 0 42.31 61.796 0.00 0.00 0.00 7.796 375 CAGTCAAGAAGCTACATCCATACACA 161 26 0 42.31 61.796 0.00 0.00 0.00 7.796 376 ACACAGTCAAGAAGCTACATCCATAC 164 26 0 42.31 61.797 0.00 0.00 0.00 7.797 377 ACAGTCAAGAAGCTACATCCATACAC 162 26 0 42.31 61.797 0.00 0.00 0.00 7.797 378 ATACACAACACACATGTATATGCACAA 142 27 0 33.33 60.828 0.00 0.00 39.26 7.828 379 TACACAACACACATGTATATGCACAAT 141 27 0 33.33 60.828 0.00 0.00 39.26 7.828 380 ACACAACACACATGTATATGCACAATA 140 27 0 33.33 60.828 0.00 0.00 39.26 7.828 381 TGTATATGCACAATACCACACATGTTT 128 27 0 33.33 60.831 15.76 0.00 0.00 7.831 382 ATATACACAACAGACACACACACACA 192 26 0 38.46 61.853 0.00 0.00 0.00 7.853 383 CACACACACACAACATACACTCTAGA 242 26 0 42.31 61.893 0.00 0.00 0.00 7.893 384 CACACACACAACATACACTCTAGACA 240 26 0 42.31 61.893 0.00 0.00 0.00 7.893 385 CACACACAACATACACTCTAGACACA 238 26 0 42.31 61.893 0.00 0.00 0.00 7.893 386 ACACACACACAACATACACTCTAGAC 241 26 0 42.31 61.894 0.00 0.00 0.00 7.894 387 ACACACACAACATACACTCTAGACAC 239 26 0 42.31 61.894 0.00 0.00 0.00 7.894 388 TACACAACAGACACACACACACAAC 189 25 0 44.00 62.964 0.00 0.00 0.00 7.964 389 ACACACACAACACACAGTCAAGAAG 175 25 0 44.00 62.976 0.00 0.00 0.00 7.976 390 AAGCTACATCCATACACAACACACAT 153 26 0 38.46 61.979 0.00 0.00 0.00 7.979 391 ATCCATACACAACACACATGTATATGC 146 27 0 37.04 60.986 2.84 0.00 39.26 7.986 392 TATGCACAATACCACACATGTTTAACA 124 27 0 33.33 60.991 0.00 0.00 0.00 7.991 393 TATACACAACAGACACACACACACAA 191 26 0 38.46 62.015 0.00 0.00 0.00 8.015 394 TCTACACACACACACAACATACACTC 246 26 0 42.31 62.110 0.00 0.00 0.00 8.110 395 CTACACACACACACAACATACACTCT 245 26 0 42.31 62.114 0.00 0.00 0.00 8.114 396 ACACACACACACAACATACACTCTAG 243 26 0 42.31 62.114 0.00 0.00 0.00 8.114 397 CACATGTATATGCACAATACCACACAT 132 27 0 37.04 61.200 15.76 5.82 38.43 8.200 398 ACATGTATATGCACAATACCACACATG 131 27 0 37.04 61.200 16.36 16.36 38.43 8.200 399 CATGTATATGCACAATACCACACATGT 130 27 0 37.04 61.200 14.06 0.00 33.01 8.200 400 TATATGCACAATACCACACATGTTTAA 126 27 0 29.63 58.771 0.00 0.00 0.00 8.229 401 AGAAGCTACATCCATACACAACACAC 155 26 0 42.31 62.236 0.00 0.00 0.00 8.236 402 GTATATACACAACAGACACACACACAC 194 27 0 40.74 61.287 8.63 0.00 0.00 8.287 403 CATACACAACACACATGTATATGCACA 143 27 0 37.04 61.352 0.00 0.00 39.26 8.352 404 CAACACACATGTATATGCACAATACCA 137 27 0 37.04 61.358 15.76 7.60 39.26 8.358 405 ACAACACACATGTATATGCACAATACC 138 27 0 37.04 61.358 15.76 3.34 39.26 8.358 406 AACACACATGTATATGCACAATACCAC 136 27 0 37.04 61.358 15.76 0.00 39.26 8.358 407 CACAATACCACACATGTTTAACAGACA 120 27 0 37.04 61.410 3.60 0.00 0.00 8.410 408 GAAGCTACATCCATACACAACACACA 154 26 0 42.31 62.452 0.00 0.00 0.00 8.452 409 AGCTACATCCATACACAACACACATG 152 26 0 42.31 62.512 0.00 0.00 0.00 8.512 410 ACAACACACAGTCAAGAAGCTACATC 169 26 0 42.31 62.610 0.00 0.00 0.00 8.610 411 TGTATATACACAACAGACACACACACA 195 27 0 37.04 61.634 16.53 0.00 0.00 8.634 412 TATATACACAACAGACACACACACACA 193 27 0 37.04 61.634 0.00 0.00 0.00 8.634 413 GCTACATCCATACACAACACACATGT 151 26 0 42.31 62.728 0.00 0.00 42.88 8.728 414 CACAACACACAGTCAAGAAGCTACAT 170 26 0 42.31 62.828 0.00 0.00 0.00 8.828 415 CCATACACAACACACATGTATATGCAC 144 27 0 40.74 61.873 2.84 0.00 39.26 8.873 416 CACACATGTATATGCACAATACCACAC 134 27 0 40.74 61.873 15.76 0.00 38.43 8.873 417 CTACACACACACACAACATACACTCTA 245 27 0 40.74 61.887 0.00 0.00 0.00 8.887 418 TACACACACACACAACATACACTCTAG 244 27 0 40.74 61.887 0.00 0.00 0.00 8.887 419 ATGCACAATACCACACATGTTTAACAG 123 27 0 37.04 61.943 0.00 0.00 0.00 8.943 420 TCCATACACAACACACATGTATATGCA 145 27 0 37.04 62.020 2.84 0.00 39.26 9.020 421 CAGTCAAGAAGCTACATCCATACACAA 161 27 0 40.74 62.221 0.00 0.00 0.00 9.221 422 AGTCAAGAAGCTACATCCATACACAAC 160 27 0 40.74 62.222 0.00 0.00 0.00 9.222 423 ACACACATGTATATGCACAATACCACA 135 27 0 37.04 62.236 15.76 4.59 38.02 9.236 424 ACACATGTATATGCACAATACCACACA 133 27 0 37.04 62.236 15.76 4.01 38.43 9.236 425 ATATACACAACAGACACACACACACAA 192 27 0 37.04 62.277 0.00 0.00 0.00 9.277 426 GATCTACACACACACACAACATACACT 248 27 0 40.74 62.366 0.00 0.00 0.00 9.366 427 ATCTACACACACACACAACATACACTC 247 27 0 40.74 62.366 0.00 0.00 0.00 9.366 428 GCACAATACCACACATGTTTAACAGAC 121 27 0 40.74 62.394 3.60 0.00 0.00 9.394 429 GTCAAGAAGCTACATCCATACACAACA 159 27 0 40.74 62.430 0.00 0.00 0.00 9.430 430 TCAAGAAGCTACATCCATACACAACAC 158 27 0 40.74 62.430 0.00 0.00 0.00 9.430 431 GCTACATCCATACACAACACACATGTA 151 27 0 40.74 62.480 0.00 0.00 39.26 9.480 432 CACACACAACATACACTCTAGACACAG 238 27 0 44.44 62.609 0.00 0.00 0.00 9.609 433 CAAGAAGCTACATCCATACACAACACA 157 27 0 40.74 62.643 0.00 0.00 0.00 9.643 434 AAGAAGCTACATCCATACACAACACAC 156 27 0 40.74 62.644 0.00 0.00 0.00 9.644 435 GAAGCTACATCCATACACAACACACAT 154 27 0 40.74 62.698 0.00 0.00 0.00 9.698 436 CACACAGTCAAGAAGCTACATCCATAC 165 27 0 44.44 62.728 0.00 0.00 0.00 9.728 437 CACAGTCAAGAAGCTACATCCATACAC 163 27 0 44.44 62.728 0.00 0.00 0.00 9.728 438 TGCACAATACCACACATGTTTAACAGA 122 27 0 37.04 62.765 3.60 0.00 0.00 9.765 439 CACACACACACAACATACACTCTAGAC 242 27 0 44.44 62.814 0.00 0.00 0.00 9.814 440 CACACACACAACATACACTCTAGACAC 240 27 0 44.44 62.814 0.00 0.00 0.00 9.814 441 AAGCTACATCCATACACAACACACATG 153 27 0 40.74 62.910 0.00 0.00 0.00 9.910 442 TATACACAACAGACACACACACACAAC 191 27 0 40.74 62.936 0.00 0.00 0.00 9.936 primer3-2.3.6/test/primer1_th_list_last/targets_test.rev0000664000077300007730000000043712231156243022415 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 CAGTGTGTACATGGCGGC 161 18 0 61.11 59.705 0.27 0.27 0.00 2.295 primer3-2.3.6/test/primer1_th_list_last/long_seq.rev0000664000077300007730000020573312231156243021522 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 ctagcatcgcctagctagca 430 20 0 55.00 60.028 19.30 4.57 31.07 0.028 1 agctagcatcgcgtatcagt 495 20 0 50.00 59.957 19.30 0.00 0.00 0.043 2 tcgcctagctagcatcgatc 424 20 0 55.00 60.100 21.96 5.00 0.00 0.100 3 gcctagctagcatcgatcga 422 20 0 55.00 60.100 22.52 22.52 0.00 0.100 4 ccgctacgatcgatcagcta 584 20 0 55.00 60.101 26.08 14.12 0.00 0.101 5 gcatcgatcgatcagtcagc 132 20 0 55.00 59.826 21.92 17.74 32.26 0.174 6 ctagctagcatcgcctagct 434 20 0 55.00 59.742 25.56 25.56 43.53 0.258 7 agctagcatcgcctagctag 432 20 0 55.00 59.742 22.37 19.25 41.54 0.258 8 agtcagctagctagatcgcc 602 20 0 55.00 59.671 12.77 4.90 0.00 0.329 9 catcgcctagctagcatcga 426 20 0 55.00 60.382 24.11 24.11 0.00 0.382 10 gctagcatcgatcgatcagc 417 20 0 55.00 59.618 24.31 24.31 32.26 0.382 11 gcatcgatcgatcagctagc 413 20 0 55.00 59.618 26.76 21.92 32.26 0.382 12 gctagcatcgcgtatcagtc 494 20 0 55.00 59.551 11.13 0.00 0.00 0.449 13 atcgcctagctagcatcgat 425 20 0 50.00 59.452 25.68 25.68 0.00 0.548 14 tcgcgtatcagtcagctagc 487 20 0 55.00 60.589 7.15 7.15 0.00 0.589 15 cgcgtatcagtcagctagct 486 20 0 55.00 60.590 16.77 15.18 0.00 0.590 16 agctagcatcgtcagctagc 670 20 0 55.00 60.594 32.27 32.27 45.11 0.594 17 gctagcatcgtcagctagct 669 20 0 55.00 60.594 32.14 15.77 43.52 0.594 18 tagcatcgcctagctagcat 429 20 0 50.00 59.375 6.22 0.00 0.00 0.625 19 tagctagccgcacatcgatc 104 20 0 55.00 60.662 12.64 0.00 42.98 0.662 20 ctagctagccgcacatcgat 105 20 0 55.00 60.663 8.73 0.00 42.98 0.663 21 agcatcgcctagctagcatc 428 20 0 55.00 60.668 4.75 0.00 0.00 0.668 22 cgcgcagagatgatagtagc 375 20 0 55.00 59.273 0.00 0.00 0.00 0.727 23 agctagtagcccagctagct 686 20 0 55.00 60.758 19.91 19.14 42.96 0.758 24 ctagctagatcgccgctacg 596 20 0 60.00 60.791 16.82 0.91 0.00 0.791 25 tagctagtagcccagctagc 687 20 0 55.00 59.013 19.24 15.24 42.96 0.987 26 gctagtagcccagctagcta 685 20 0 55.00 59.013 18.14 12.57 42.96 0.987 27 gtcagctagctagatcgccg 601 20 0 60.00 61.003 10.01 0.00 0.00 1.003 28 gctagctacgatcgatcagc 623 20 0 55.00 58.995 24.56 24.39 0.00 1.005 29 gctacgatcgatcagctagc 582 20 0 55.00 58.995 24.59 21.38 0.00 1.005 30 tagccgcacatcgatcgatc 100 20 0 55.00 61.006 16.19 16.29 42.05 1.006 31 ctagccgcacatcgatcgat 101 20 0 55.00 61.008 25.37 25.37 42.05 1.008 32 gcatcgcgtatcagtcagc 490 19 0 57.89 60.025 0.00 0.00 0.00 1.025 33 agcatcgatgatcagctagct 210 21 0 47.62 60.035 17.33 15.38 0.00 1.035 34 tagccgcacatcgatcgat 100 19 0 52.63 59.945 25.37 25.37 42.05 1.055 35 gctagctagatcgccgctac 597 20 0 60.00 61.071 25.76 2.00 0.00 1.071 36 cgatcgatcagtcagctagc 128 20 0 55.00 58.924 16.29 7.15 0.00 1.076 37 ctagctagtagcccagctagc 688 21 0 57.14 60.104 14.66 14.92 42.96 1.104 38 gctagtagcccagctagctag 685 21 0 57.14 60.104 19.33 19.33 44.03 1.104 39 tagctagcatcgcgtatcagt 496 21 0 47.62 59.765 19.30 0.00 0.00 1.235 40 agctagcatcgatcgatcagt 137 21 0 47.62 59.763 21.16 14.32 32.26 1.237 41 ctagctagccgcacatcga 105 19 0 57.89 60.242 8.73 0.00 42.98 1.242 42 agctagctagatcgccgcta 598 20 0 55.00 61.243 25.76 12.01 36.75 1.243 43 tagctagatcgccgctacg 595 19 0 57.89 59.729 14.08 0.00 0.00 1.271 44 cgctacgatcgatcagctag 583 20 0 55.00 58.727 26.77 15.91 0.00 1.273 45 gctagctagatcgccgcta 597 19 0 57.89 59.725 25.76 12.01 0.00 1.275 46 ccgctacgatcgatcagct 584 19 0 57.89 60.315 26.08 13.79 0.00 1.315 47 tcagctagctagatcgccg 600 19 0 57.89 59.650 15.67 3.09 0.00 1.350 48 gctagcatcgcctagctagc 431 20 0 60.00 61.357 24.49 24.49 44.24 1.357 49 cgcgtatcagtcagctagcta 486 21 0 52.38 60.373 4.08 0.00 0.00 1.373 50 tcgatcgatcagtcagctagc 129 21 0 52.38 60.374 19.14 7.15 0.00 1.374 51 cgatcgatcagtcagctagct 128 21 0 52.38 60.374 16.29 15.18 0.00 1.374 52 tagctagcatcgtcagctagc 671 21 0 52.38 60.375 32.27 32.27 45.11 1.375 53 gctagcatcgtcagctagcta 669 21 0 52.38 60.375 32.14 16.02 43.52 1.375 54 cgcctagctagcatcgatcg 423 20 0 60.00 61.409 18.32 9.87 0.00 1.409 55 tcgactagctagctacgatcg 630 21 0 52.38 59.573 25.10 25.04 0.00 1.427 56 cgactagctagctacgatcga 629 21 0 52.38 59.573 25.89 10.02 0.00 1.427 57 tagctagccgcacatcgat 104 19 0 52.63 59.568 12.64 0.00 42.98 1.432 58 agcatcgcctagctagcat 428 19 0 52.63 59.561 6.22 0.00 0.00 1.439 59 agctagctacgatcgatcagc 624 21 0 52.38 60.441 24.24 24.24 0.00 1.441 60 gctagctacgatcgatcagct 623 21 0 52.38 60.441 32.07 32.07 35.03 1.441 61 gctacgatcgatcagctagct 582 21 0 52.38 60.441 24.35 15.18 0.00 1.441 62 ctagctagcatcgcctagcta 434 21 0 52.38 59.559 26.56 25.32 42.44 1.441 63 tagctagcatcgcctagctag 433 21 0 52.38 59.559 26.48 21.23 42.44 1.441 64 tagcatcgcctagctagcatc 429 21 0 52.38 60.444 4.75 0.00 0.00 1.444 65 ctagcatcgcctagctagcat 430 21 0 52.38 60.444 8.05 0.00 31.07 1.444 66 agcatcgatgatcagctagc 210 20 0 50.00 58.539 17.33 10.73 0.00 1.461 67 gcatcgatgatcagctagct 209 20 0 50.00 58.539 29.80 15.18 0.00 1.461 68 agcatcgcgtatcagtcagc 491 20 0 55.00 61.494 0.00 0.00 0.00 1.494 69 gcatcgcgtatcagtcagct 490 20 0 55.00 61.494 0.00 0.00 0.00 1.494 70 tagtcagctagctagatcgcc 603 21 0 52.38 59.491 12.77 4.90 0.00 1.509 71 atcgcctagctagcatcgatc 425 21 0 52.38 60.510 25.68 7.98 30.32 1.510 72 gcctagctagcatcgatcgat 422 21 0 52.38 60.510 25.37 25.37 35.13 1.510 73 tagctagtagcccagctagct 687 21 0 52.38 60.522 23.76 23.76 42.96 1.522 74 agctagtagcccagctagcta 686 21 0 52.38 60.522 20.42 12.57 42.96 1.522 75 tagctagcatcgcctagcta 433 20 0 50.00 58.433 27.88 26.05 42.44 1.567 76 agctagcatgatcgactagct 641 21 0 47.62 59.415 27.53 27.53 41.39 1.585 77 agcatgatcgactagctagct 637 21 0 47.62 59.415 23.74 23.74 0.00 1.585 78 agcatcgatcagtagctagct 323 21 0 47.62 59.415 23.74 23.74 32.09 1.585 79 ctagccgcacatcgatcga 101 19 0 57.89 60.606 22.52 22.52 42.05 1.606 80 gctagcatcgatcgatcagtc 136 21 0 52.38 59.378 21.16 13.80 32.26 1.622 81 tgatcgatcagtcagctagct 280 21 0 47.62 59.347 26.87 15.18 32.65 1.653 82 ctagcatcgcgtatcagtcag 493 21 0 52.38 59.312 0.00 0.00 0.00 1.688 83 catcgcgtatcagtcagctag 489 21 0 52.38 59.312 0.00 0.00 0.00 1.688 84 tcgcgcagagatgatagtagc 376 21 0 52.38 60.707 5.16 0.00 0.00 1.707 85 cgcgcagagatgatagtagct 375 21 0 52.38 60.709 0.00 0.00 0.00 1.709 86 gctagcatcgatcgatcagt 136 20 0 50.00 58.270 21.16 14.32 32.26 1.730 87 catcgcctagctagcatcgat 426 21 0 52.38 60.779 25.68 25.68 33.48 1.779 88 ctagcatcgcgtatcagtca 493 20 0 50.00 58.207 0.00 0.00 0.00 1.793 89 tagcatcgcgtatcagtcag 492 20 0 50.00 58.207 0.00 0.00 0.00 1.793 90 catcgcgtatcagtcagcta 489 20 0 50.00 58.207 0.00 0.00 0.00 1.793 91 agcatcgatcgatcagtcag 133 20 0 50.00 58.197 24.35 15.60 32.26 1.803 92 catcgatcgatcagtcagct 131 20 0 50.00 58.197 17.10 0.00 32.26 1.803 93 agctagtagcccagctagc 686 19 0 57.89 59.181 19.24 15.24 42.96 1.819 94 gctagtagcccagctagct 685 19 0 57.89 59.181 15.57 14.94 42.96 1.819 95 agctacgatcgatcagctagt 620 21 0 47.62 59.147 28.83 14.96 31.57 1.853 96 gcatcgcctagctagcatc 427 19 0 57.89 59.141 2.42 0.00 0.00 1.859 97 ctagctagcatcgcgtatcag 497 21 0 52.38 59.113 8.73 0.00 0.00 1.887 98 cgactagctagctacgatcg 629 20 0 55.00 58.105 21.59 21.59 0.00 1.895 99 agctagccgcacatcgatc 103 19 0 57.89 60.905 12.64 0.00 42.98 1.905 100 cgcgtatcagtcagctagc 486 19 0 57.89 59.086 7.15 7.15 0.00 1.914 101 gctagcatcgtcagctagc 669 19 0 57.89 59.076 28.98 28.98 41.90 1.924 102 ctagcatcgatcgcgcagag 386 20 0 60.00 61.946 20.72 15.36 37.79 1.946 103 ctagcatcgatcgcgcaga 386 19 0 57.89 60.966 20.72 11.52 37.79 1.966 104 tagcatcgatcgcgcagag 385 19 0 57.89 60.966 25.40 8.20 37.79 1.966 105 gcatcgcctagctagcatcg 427 20 0 60.00 61.968 9.94 7.60 0.00 1.968 106 agctagcatcgcgtatcagtc 495 21 0 52.38 60.975 19.30 0.00 0.00 1.975 107 atcgcgtatcagtcagctagc 488 21 0 52.38 60.975 7.15 7.15 0.00 1.975 108 ctagctagcatcgcgtatca 497 20 0 50.00 57.994 8.73 0.00 0.00 2.006 109 tagctagcatcgcgtatcag 496 20 0 50.00 57.994 19.30 0.00 0.00 2.006 110 ctagctagcatcgatcgcgc 390 20 0 60.00 62.011 18.83 18.83 37.79 2.011 111 agctagcatcgatcgatcag 418 20 0 50.00 57.983 19.62 17.44 32.26 2.017 112 ctagcatcgatcgatcagct 416 20 0 50.00 57.983 33.38 33.38 38.16 2.017 113 agcatcgatcgatcagctag 414 20 0 50.00 57.983 30.67 19.74 34.69 2.017 114 catcgatcgatcagctagct 412 20 0 50.00 57.983 25.58 15.18 32.26 2.017 115 agctagcatcgatcgatcag 137 20 0 50.00 57.983 19.62 17.44 32.26 2.017 116 atcgactagctagctacgatcg 631 22 0 50.00 59.982 29.34 25.04 35.52 2.018 117 cgactagctagctacgatcgat 629 22 0 50.00 59.982 25.89 10.20 0.00 2.018 118 atcgcctagctagcatcga 425 19 0 52.63 58.964 24.11 24.11 0.00 2.036 119 tcgcctagctagcatcgat 424 19 0 52.63 58.964 21.96 0.00 0.00 2.036 120 tagctagcatcgatcgcgc 389 19 0 57.89 61.037 18.83 18.83 37.79 2.037 121 agctagcatcgatcgatcagc 418 21 0 52.38 61.044 27.48 27.48 32.26 2.044 122 gctagcatcgatcgatcagct 417 21 0 52.38 61.044 28.28 28.28 38.16 2.044 123 agcatcgatcgatcagctagc 414 21 0 52.38 61.044 30.67 26.27 34.69 2.044 124 gcatcgatcgatcagctagct 413 21 0 52.38 61.044 26.76 15.18 32.26 2.044 125 ctagctagcatcgatcgcg 390 19 0 57.89 58.947 12.72 4.04 39.35 2.053 126 agctagatcgccgctacg 594 18 0 61.11 59.935 5.36 0.00 0.00 2.065 127 agctagctagatcgccgc 598 18 0 61.11 59.933 25.76 8.52 0.00 2.067 128 gctagctagatcgccgct 597 18 0 61.11 59.933 25.76 11.03 0.00 2.067 129 atcgcgtatcagtcagctag 488 20 0 50.00 57.918 0.00 0.00 0.00 2.082 130 ccgctacgatcgatcagctag 584 21 0 57.14 61.096 26.77 19.04 0.00 2.096 131 agctagcatgatcgactagc 641 20 0 50.00 57.898 24.35 24.35 38.69 2.102 132 gctagcatgatcgactagct 640 20 0 50.00 57.898 24.41 14.30 37.17 2.102 133 agcatgatcgactagctagc 637 20 0 50.00 57.898 12.80 7.15 0.00 2.102 134 gcatgatcgactagctagct 636 20 0 50.00 57.898 23.74 23.74 0.00 2.102 135 gcgcagagatgatagtagct 374 20 0 50.00 57.898 0.67 0.00 0.00 2.102 136 agcatcgatcagtagctagc 323 20 0 50.00 57.898 7.15 7.15 32.09 2.102 137 gcatcgatcagtagctagct 322 20 0 50.00 57.898 23.74 23.74 0.00 2.102 138 tagcatcgcctagctagca 429 19 0 52.63 58.881 19.30 4.57 0.00 2.119 139 tagcatcgatcgcgcaga 385 18 0 55.56 59.857 25.40 14.05 37.79 2.143 140 catcgcctagctagcatcg 426 19 0 57.89 58.856 9.94 7.51 0.00 2.144 141 tagcatcgatgatcagctagct 211 22 0 45.45 59.845 20.84 20.84 0.00 2.155 142 agcatcgatgatcagctagcta 210 22 0 45.45 59.845 16.83 0.00 0.00 2.155 143 tgatcgatcagtcagctagc 280 20 0 50.00 57.829 26.87 7.15 32.65 2.171 144 agctacgatcgatcagctagtc 620 22 0 50.00 60.174 28.83 14.40 31.57 2.174 145 cgatcgatcagtcagctagcta 128 22 0 50.00 60.174 16.29 0.00 0.00 2.174 146 agcatcgatgatgatcagct 534 20 0 45.00 57.788 15.88 3.53 42.87 2.212 147 gctacgatcgatcagctagtc 619 21 0 52.38 58.780 24.45 12.10 0.00 2.220 148 atgatcgatcagtcagctagct 281 22 0 45.45 59.778 30.77 15.18 41.24 2.222 149 agctagccgcacatcgat 103 18 0 55.56 59.771 12.64 0.00 42.98 2.229 150 gctagcatcgcgtatcagtca 494 21 0 52.38 61.238 11.13 0.00 0.00 2.238 151 tagcatcgcgtatcagtcagc 492 21 0 52.38 61.238 0.00 0.00 0.00 2.238 152 gcatcgcgtatcagtcagcta 490 21 0 52.38 61.238 0.00 0.00 0.00 2.238 153 tagctagctacgatcgatcagc 625 22 0 50.00 60.238 27.80 24.24 0.00 2.238 154 gctagctacgatcgatcagcta 623 22 0 50.00 60.238 32.87 31.34 33.83 2.238 155 gctacgatcgatcagctagcta 582 22 0 50.00 60.238 24.35 11.52 0.00 2.238 156 cctagctagcatcgatcgatca 421 22 0 50.00 60.239 24.35 14.31 32.26 2.239 157 agcatcgatcgatcagtcagc 133 21 0 52.38 61.244 24.35 22.21 32.26 2.244 158 gcatcgatcgatcagtcagct 132 21 0 52.38 61.244 21.92 1.18 32.26 2.244 159 gctagtagcatcgatgatcagc 216 22 0 50.00 59.727 20.63 18.65 44.86 2.273 160 gtagcatcgatgatcagctagc 212 22 0 50.00 59.727 13.01 5.79 29.76 2.273 161 gcgtatcagtcagctagctag 485 21 0 52.38 58.703 17.46 17.46 0.00 2.297 162 gctagcatcgatgatgatcagc 537 22 0 50.00 60.302 15.09 11.65 42.87 2.302 163 gcatcgatgatgatcagctagc 533 22 0 50.00 60.302 29.80 4.67 42.87 2.302 164 tagctagtagcccagctagcta 687 22 0 50.00 60.308 26.09 24.26 42.96 2.308 165 tcgccgctacgatcgatc 587 18 0 61.11 60.318 16.29 16.29 37.75 2.318 166 cagctagctagatcgccgc 599 19 0 63.16 61.329 13.18 0.00 0.00 2.329 167 tcgcgcagagatgatagtag 376 20 0 50.00 57.636 5.16 0.00 0.00 2.364 168 gctacgatcgatcagctagt 619 20 0 50.00 57.634 24.45 14.25 0.00 2.366 169 gcgtatcagtcagctagctagt 485 22 0 50.00 60.368 22.18 6.94 0.00 2.368 170 tagcatcgatcgcgcagaga 385 20 0 55.00 62.413 25.40 10.09 43.32 2.413 171 actagctagcatcgatcgatca 140 22 0 45.45 59.586 24.35 14.31 32.26 2.414 172 tagctagcatcgatcgatcagt 138 22 0 45.45 59.586 19.30 8.52 32.26 2.414 173 tagctagcatcgcctagct 433 19 0 52.63 58.572 25.56 25.56 43.53 2.428 174 agctagcatcgcctagcta 432 19 0 52.63 58.572 22.37 12.23 41.54 2.428 175 gctacgatcgatcagctagtca 619 22 0 50.00 60.431 24.45 2.69 0.00 2.431 176 cctagctagcatcgatcgatc 421 21 0 52.38 58.567 20.96 16.29 32.26 2.433 177 cgcctagctagcatcgatc 423 19 0 57.89 58.563 18.32 2.36 0.00 2.437 178 gcctagctagcatcgatcg 422 19 0 57.89 58.563 12.69 9.87 0.00 2.437 179 acgatcgatcagctagtcag 616 20 0 50.00 57.559 24.83 4.59 0.00 2.441 180 gcgtatcagtcagctagcta 485 20 0 50.00 57.549 4.08 0.00 0.00 2.451 181 gatcgatcagtcagctagct 279 20 0 50.00 57.536 21.11 15.18 0.00 2.464 182 gatcgatcagtcagctagct 127 20 0 50.00 57.536 21.11 15.18 0.00 2.464 183 tgatcgactagctagctacga 633 21 0 47.62 58.535 29.72 29.72 34.25 2.465 184 gcatgatgatcgatcagtcagc 286 22 0 50.00 60.493 27.06 25.12 45.35 2.493 185 cgcgcagagatgatagtagcta 375 22 0 50.00 60.495 0.00 0.00 0.00 2.495 186 ctagtcagctagctagatcgcc 604 22 0 54.55 60.496 12.77 4.90 39.03 2.496 187 agctagctagatcgccgct 598 19 0 57.89 61.519 25.76 10.93 34.45 2.519 188 gcatcgatgatcagctagctag 209 22 0 50.00 59.470 29.80 17.46 0.00 2.530 189 tagccgcacatcgatcga 100 18 0 55.56 59.465 22.52 22.52 42.05 2.535 190 ctagctagcatcgcctagctag 434 22 0 54.55 60.561 30.91 30.91 46.84 2.561 191 ctagctagcatcgtcagctag 672 21 0 52.38 58.431 17.15 17.15 33.70 2.569 192 ctagcatcgtcagctagctag 668 21 0 52.38 58.431 17.46 17.46 35.38 2.569 193 ctagcatcgatcgcgcag 386 18 0 61.11 59.415 18.08 11.93 37.79 2.585 194 tagcatcgatgatcagctagc 211 21 0 47.62 58.410 20.20 11.56 0.00 2.590 195 gcatcgatgatcagctagcta 209 21 0 47.62 58.410 29.80 0.00 0.00 2.590 196 gctagcatcgcgtatcagt 494 19 0 52.63 58.407 11.13 0.00 0.00 2.593 197 gatgatcgatcagtcagctagc 282 22 0 50.00 59.406 26.17 9.18 41.24 2.594 198 cctagctagcatcgatcgat 421 20 0 50.00 57.396 25.37 25.37 35.13 2.604 199 cgccgctacgatcgatca 586 18 0 61.11 60.621 24.95 6.39 34.47 2.621 200 ctagctagccgcacatcgatc 105 21 0 57.14 61.630 8.73 0.00 42.98 2.630 201 agctagctacgatcgatcag 624 20 0 50.00 57.344 18.86 13.89 0.00 2.656 202 ctagctacgatcgatcagct 622 20 0 50.00 57.344 32.07 32.07 35.03 2.656 203 agctacgatcgatcagctag 620 20 0 50.00 57.344 28.83 15.18 31.57 2.656 204 ctacgatcgatcagctagct 581 20 0 50.00 57.344 16.25 15.18 0.00 2.656 205 acgatcgatcagctagctag 579 20 0 50.00 57.344 24.83 17.46 0.00 2.656 206 atgatcgatcagtcagctagc 281 21 0 47.62 58.339 30.77 7.15 41.24 2.661 207 cgccgctacgatcgatcag 586 19 0 63.16 61.664 18.86 13.89 34.47 2.664 208 agcatcgcgtatcagtcag 491 19 0 52.63 58.330 0.00 0.00 0.00 2.670 209 catcgcgtatcagtcagct 489 19 0 52.63 58.330 0.00 0.00 0.00 2.670 210 gctagccgcacatcgatc 102 18 0 61.11 59.329 2.83 0.00 42.05 2.671 211 cgctacgatcgatcagctagc 583 21 0 57.14 61.676 24.77 23.30 0.00 2.676 212 tcgatcgatcagtcagctag 129 20 0 50.00 57.276 19.14 2.65 0.00 2.724 213 ctagctagcatcgtcagcta 672 20 0 50.00 57.257 8.73 6.89 0.00 2.743 214 tagctagcatcgtcagctag 671 20 0 50.00 57.257 21.54 21.54 33.70 2.743 215 ctagcatcgtcagctagcta 668 20 0 50.00 57.257 16.85 6.25 0.00 2.743 216 tagcatcgtcagctagctag 667 20 0 50.00 57.257 17.46 17.46 0.00 2.743 217 tagctagcatgatcgactagct 642 22 0 45.45 59.252 31.28 31.28 44.76 2.748 218 agctagcatgatcgactagcta 641 22 0 45.45 59.252 28.71 17.80 42.99 2.748 219 tagcatgatcgactagctagct 638 22 0 45.45 59.252 23.74 23.74 33.71 2.748 220 agcatgatcgactagctagcta 637 22 0 45.45 59.252 25.55 23.56 0.00 2.748 221 tagcatcgatcagtagctagct 324 22 0 45.45 59.252 23.74 23.74 34.70 2.748 222 agcatcgatcagtagctagcta 323 22 0 45.45 59.252 23.52 19.77 32.09 2.748 223 tagctagcatcgcgtatcagtc 496 22 0 50.00 60.751 19.30 0.00 0.00 2.751 224 ctagctagcatcgcgtatcagt 497 22 0 50.00 60.752 8.73 0.00 0.00 2.752 225 agctagcatcgatcgatcagtc 137 22 0 50.00 60.753 19.30 11.46 32.26 2.753 226 atcgatcgatcagtcagctagc 130 22 0 50.00 60.753 25.37 7.15 0.00 2.753 227 tagctagcatcgatcgcgca 389 20 0 55.00 62.756 25.40 14.07 37.79 2.756 228 tcagctagctagatcgccgc 600 20 0 60.00 62.765 15.67 0.00 0.00 2.765 229 cagctagctagatcgccgct 599 20 0 60.00 62.771 13.18 8.42 38.13 2.771 230 tgatcgatcagtcagctagcta 280 22 0 45.45 59.187 26.87 0.00 32.65 2.813 231 tagctagcatcgatcgatcagc 419 22 0 50.00 60.816 27.48 27.48 32.26 2.816 232 gctagcatcgatcgatcagcta 417 22 0 50.00 60.816 29.31 28.09 36.80 2.816 233 tagcatcgatcgatcagctagc 415 22 0 50.00 60.816 34.03 27.91 36.80 2.816 234 gcatcgatcgatcagctagcta 413 22 0 50.00 60.816 26.76 2.25 32.26 2.816 235 ctagctagcatcgcctagc 434 19 0 57.89 58.179 17.34 17.34 36.95 2.821 236 gctagcatcgcctagctag 431 19 0 57.89 58.179 17.61 17.08 35.42 2.821 237 ctagcatcgcctagctagc 430 19 0 57.89 58.179 10.43 7.15 31.07 2.821 238 ctagtcagctagctagatcgc 604 21 0 52.38 58.161 12.77 5.13 39.03 2.839 239 ctagcatcgatcgatcagtcag 135 22 0 50.00 59.155 18.38 11.05 32.26 2.845 240 catcgatcgatcagtcagctag 131 22 0 50.00 59.155 17.10 0.68 32.26 2.845 241 gcatgatcgactagctagctac 636 22 0 50.00 59.151 13.83 3.08 0.00 2.849 242 gtagcatcgatcagtagctagc 325 22 0 50.00 59.151 7.27 7.15 35.96 2.849 243 agtagcatcgatgatcagct 213 20 0 45.00 57.134 20.75 13.47 0.00 2.866 244 agctagcatcgcgtatcag 495 19 0 52.63 58.107 19.30 0.00 0.00 2.893 245 gtcagctagctagatcgcc 601 19 0 57.89 58.106 10.01 0.00 0.00 2.894 246 gctagctagccgcacatcg 106 19 0 63.16 61.902 32.38 0.17 43.93 2.902 247 ctagcatcgatcgatcagtca 135 21 0 47.62 58.084 18.38 7.20 32.26 2.916 248 tagcatcgatcgatcagtcag 134 21 0 47.62 58.084 24.35 15.60 32.26 2.916 249 catcgatcgatcagtcagcta 131 21 0 47.62 58.084 17.10 0.00 32.26 2.916 250 gctagcatgatgatcgatcagt 290 22 0 45.45 59.071 12.39 3.56 45.35 2.929 251 tagctagccgcacatcga 104 18 0 55.56 59.064 12.64 0.00 42.98 2.936 252 tgatgatcgatcagtcagct 283 20 0 45.00 57.064 28.37 16.33 41.24 2.936 253 tcgatcgatcagctagctag 410 20 0 50.00 57.060 18.63 17.46 0.00 2.940 254 ctagccgcacatcgatcgatc 101 21 0 57.14 61.947 16.19 16.29 42.05 2.947 255 tcgactagctagctacgatcga 630 22 0 50.00 60.949 29.09 28.16 0.00 2.949 256 agcatcgcctagctagca 428 18 0 55.56 59.050 19.30 4.57 0.00 2.950 257 gccgctacgatcgatcagc 585 19 0 63.16 61.960 23.65 22.94 0.00 2.960 258 ctagccgcacatcgatcg 101 18 0 61.11 59.032 9.87 9.87 42.05 2.968 259 agcatgatgatcgatcagtcag 287 22 0 45.45 59.004 23.86 19.51 45.35 2.996 260 catgatgatcgatcagtcagct 285 22 0 45.45 59.004 22.18 12.60 45.35 2.996 261 tcgcgtatcagtcagctagct 487 21 0 52.38 62.000 16.77 15.18 0.00 3.000 262 atgatcgactagctagctacga 634 22 0 45.45 58.997 29.72 29.72 34.25 3.003 263 tgatcgactagctagctacgat 633 22 0 45.45 58.997 34.20 34.20 39.30 3.003 264 actagctagctacgatcgatca 627 22 0 45.45 58.997 24.95 6.39 0.00 3.003 265 tagctacgatcgatcagctagt 621 22 0 45.45 58.997 32.79 21.54 33.83 3.003 266 gctagcatcgatcgatcagtca 136 22 0 50.00 61.008 16.51 6.56 32.26 3.008 267 tagcatcgatcgatcagtcagc 134 22 0 50.00 61.008 24.35 22.21 32.26 3.008 268 gcatcgatcgatcagtcagcta 132 22 0 50.00 61.008 21.92 3.26 32.26 3.008 269 tgatcgactagctagctacgatc 633 23 0 47.83 59.990 39.87 39.87 44.89 3.010 270 gactagctagctacgatcgatca 628 23 0 47.83 59.990 24.95 6.39 0.00 3.010 271 tagctacgatcgatcagctagtc 621 23 0 47.83 59.990 32.79 19.29 33.83 3.010 272 actagctagctacgatcgatcag 627 23 0 47.83 59.990 24.00 13.70 0.00 3.010 273 ctagctacgatcgatcagctagt 622 23 0 47.83 59.990 38.29 26.57 40.64 3.010 274 ctagctagcatcgatcgatcag 420 22 0 50.00 58.963 19.62 17.44 32.26 3.037 275 ctagcatcgatcgatcagctag 416 22 0 50.00 58.963 37.86 37.86 43.17 3.037 276 catcgatcgatcagctagctag 412 22 0 50.00 58.963 25.58 17.46 32.26 3.037 277 ctagctagcatcgatcgatcag 139 22 0 50.00 58.963 19.62 17.44 32.26 3.037 278 gctagcatgatgatcgatcagtc 290 23 0 47.83 60.053 25.60 21.92 45.35 3.053 279 gctagctagccgcacatc 106 18 0 61.11 58.936 32.38 5.48 43.93 3.064 280 ctagctagatcgccgctac 596 19 0 57.89 57.903 16.82 0.00 0.00 3.097 281 catgatcgactagctagctacg 635 22 0 50.00 58.902 21.95 21.95 0.00 3.098 282 agcatgatgatcgatcagtca 287 21 0 42.86 57.900 28.37 27.49 45.35 3.100 283 ctagctagcatgatcgactagc 643 22 0 50.00 58.892 24.35 24.35 38.69 3.108 284 gctagcatgatcgactagctag 640 22 0 50.00 58.892 24.41 21.28 44.82 3.108 285 ctagcatgatcgactagctagc 639 22 0 50.00 58.892 17.67 6.80 40.71 3.108 286 gcgcagagatgatagtagctag 374 22 0 50.00 58.892 0.67 0.00 0.00 3.108 287 cgcagagatgatagtagctagc 373 22 0 50.00 58.892 7.15 7.15 0.00 3.108 288 gcatcgatcagtagctagctag 322 22 0 50.00 58.892 24.35 17.46 0.00 3.108 289 ctagctagcatcgatcgatca 420 21 0 47.62 57.879 24.35 14.31 32.26 3.121 290 tagctagcatcgatcgatcag 419 21 0 47.62 57.879 19.62 17.44 32.26 3.121 291 ctagcatcgatcgatcagcta 416 21 0 47.62 57.879 34.11 32.56 36.80 3.121 292 tagcatcgatcgatcagctag 415 21 0 47.62 57.879 34.03 21.94 36.80 3.121 293 catcgatcgatcagctagcta 412 21 0 47.62 57.879 25.58 0.00 32.26 3.121 294 ctagctagcatcgatcgatca 139 21 0 47.62 57.879 24.35 14.31 32.26 3.121 295 tagctagcatcgatcgatcag 138 21 0 47.62 57.879 19.62 17.44 32.26 3.121 296 actagctagcatcgatcgatc 140 21 0 47.62 57.878 20.96 16.29 32.26 3.122 297 tcgccgctacgatcgatca 587 19 0 57.89 62.144 24.95 6.39 37.75 3.144 298 gcgtatcagtcagctagctagta 485 23 0 47.83 60.176 2.33 0.00 0.00 3.176 299 agctagcatcgatgatgatcag 538 22 0 45.45 58.807 19.30 2.54 42.87 3.193 300 ctagcatcgatgatgatcagct 536 22 0 45.45 58.807 11.21 11.21 42.87 3.193 301 agcatcgatgatgatcagctag 534 22 0 45.45 58.807 15.88 0.00 42.87 3.193 302 catcgatgatgatcagctagct 532 22 0 45.45 58.807 21.68 15.18 42.87 3.193 303 agctagcatgatgatcgatcag 291 22 0 45.45 58.807 30.77 19.47 45.35 3.193 304 atcgatcgatcagtcagctag 130 21 0 47.62 57.806 25.37 2.65 0.00 3.194 305 tgatcgatcagtcagctagctaa 280 23 0 43.48 59.798 26.87 0.00 32.65 3.202 306 tagctagcatgatcgactagc 642 21 0 47.62 57.798 24.35 24.35 38.69 3.202 307 gctagcatgatcgactagcta 640 21 0 47.62 57.798 24.41 15.59 37.17 3.202 308 tagcatgatcgactagctagc 638 21 0 47.62 57.798 12.80 7.15 33.71 3.202 309 gcatgatcgactagctagcta 636 21 0 47.62 57.798 25.55 23.56 0.00 3.202 310 gcgcagagatgatagtagcta 374 21 0 47.62 57.798 0.67 0.00 0.00 3.202 311 tagcatcgatcagtagctagc 324 21 0 47.62 57.798 11.69 7.15 34.70 3.202 312 gcatcgatcagtagctagcta 322 21 0 47.62 57.798 23.52 19.77 0.00 3.202 313 gcatcgatcgcgcagaga 383 18 0 61.11 61.229 20.78 0.00 43.32 3.229 314 agcatcgatcgcgcagag 384 18 0 61.11 61.232 25.40 8.20 37.79 3.232 315 catgatcgactagctagctacga 635 23 0 47.83 60.238 29.72 29.72 34.25 3.238 316 ctagctagcatgatcgactagct 643 23 0 47.83 60.238 31.28 31.28 44.76 3.238 317 agctagcatgatcgactagctag 641 23 0 47.83 60.238 28.71 21.28 42.99 3.238 318 ctagcatgatcgactagctagct 639 23 0 47.83 60.238 23.74 23.74 40.71 3.238 319 cgcagagatgatagtagctagct 373 23 0 47.83 60.238 23.74 23.74 0.00 3.238 320 agcatcgatcagtagctagctag 323 23 0 47.83 60.238 24.35 17.46 32.09 3.238 321 gctagccgcacatcgatcg 102 19 0 63.16 62.244 9.87 9.87 42.05 3.244 322 gccgctacgatcgatcag 585 18 0 61.11 58.727 18.86 13.89 0.00 3.273 323 ccgctacgatcgatcagc 584 18 0 61.11 58.727 23.65 22.94 0.00 3.273 324 agctagcatcgcctagct 432 18 0 55.56 58.725 21.82 21.11 39.84 3.275 325 agctagcatcgatcgcgc 388 18 0 61.11 61.306 18.83 18.83 37.79 3.306 326 agctagcatcgatgatgatca 538 21 0 42.86 57.691 19.69 0.97 42.87 3.309 327 tagcatcgatgatgatcagct 535 21 0 42.86 57.691 18.06 11.21 42.87 3.309 328 agcatcgatgatgatcagcta 534 21 0 42.86 57.691 15.88 0.00 42.87 3.309 329 agctagcatgatgatcgatca 291 21 0 42.86 57.691 28.62 28.62 45.96 3.309 330 cgcgtatcagtcagctagctag 486 22 0 54.55 61.313 17.46 17.46 0.00 3.313 331 ctagctagcatcgtcagctagc 672 22 0 54.55 61.322 32.27 32.27 45.11 3.322 332 gctagcatcgtcagctagctag 669 22 0 54.55 61.322 32.14 22.15 43.52 3.322 333 tagcatcgatgatcagctagcta 211 23 0 43.48 59.671 23.46 21.42 0.00 3.329 334 gcgtatcagtcagctagct 485 19 0 52.63 57.642 16.77 15.18 0.00 3.358 335 ctagctagccgcacatcg 105 18 0 61.11 58.638 8.73 0.00 42.98 3.362 336 atgatgatcgatcagtcagct 284 21 0 42.86 57.616 29.63 19.24 45.35 3.384 337 tcgatgatgatcagctagctagt 530 23 0 43.48 59.607 22.18 7.88 32.11 3.393 338 atgatcgatcagtcagctagcta 281 23 0 43.48 59.607 30.77 5.74 41.24 3.393 339 ctagcatcgcctagctagcatc 430 22 0 54.55 61.393 8.05 0.00 31.07 3.393 340 agtcagctagctagatcgccg 602 21 0 57.14 62.394 12.77 2.27 0.00 3.394 341 atcgatcgatcagctagctag 411 21 0 47.62 57.600 25.37 17.46 0.00 3.400 342 ctacgatcgatcagctagtcag 618 22 0 50.00 58.580 17.45 6.71 0.00 3.420 343 agctagcatgatgatcgatcagt 291 23 0 43.48 60.428 19.30 3.56 45.35 3.428 344 gatcgatcagtcagctagctag 127 22 0 50.00 58.567 21.11 17.46 0.00 3.433 345 gcatgatgatcgatcagtcag 286 21 0 47.62 57.562 23.86 19.51 45.35 3.438 346 catgatgatcgatcagtcagc 285 21 0 47.62 57.562 22.18 12.73 45.35 3.438 347 gcctagctagcatcgatcgatc 422 22 0 54.55 61.448 20.96 16.29 32.26 3.448 348 cagctagctagatcgccgcta 599 21 0 57.14 62.457 13.18 12.01 36.75 3.457 349 agctagctagatcgccgctac 598 21 0 57.14 62.458 25.76 6.53 36.75 3.458 350 atgatcgactagctagctacg 634 21 0 47.62 57.540 21.95 21.95 0.00 3.460 351 cgctacgatcgatcagcta 583 19 0 52.63 57.539 26.08 14.12 0.00 3.461 352 agcatgatcgactagctagctac 637 23 0 47.83 60.487 13.83 3.08 0.00 3.487 353 gtagcatcgatcagtagctagct 325 23 0 47.83 60.487 23.74 23.74 35.96 3.487 354 ctagctagtagcccagctagct 688 22 0 54.55 61.496 23.76 23.76 42.96 3.496 355 agctagtagcccagctagctag 686 22 0 54.55 61.496 20.42 19.33 44.03 3.496 356 agcatactagcatcgatcgat 346 21 0 42.86 57.502 25.37 25.37 37.20 3.498 357 gatgtagcatcgatcagtagct 328 22 0 45.45 58.484 10.18 10.18 36.12 3.516 358 ctacgatcgatcagctagtca 618 21 0 47.62 57.474 17.45 2.91 0.00 3.526 359 tacgatcgatcagctagtcag 617 21 0 47.62 57.474 11.44 0.00 0.00 3.526 360 tagctagcatcgatcgatcagtc 138 23 0 47.83 60.546 19.30 11.46 32.26 3.546 361 actagctagcatcgatcgatcag 140 23 0 47.83 60.547 21.52 17.44 32.26 3.547 362 ctagctagcatcgatcgatcagt 139 23 0 47.83 60.547 14.05 8.52 32.26 3.547 363 gatcgatcagtcagctagcta 279 21 0 47.62 57.451 21.11 0.00 0.00 3.549 364 gatcgatcagtcagctagcta 127 21 0 47.62 57.451 21.11 0.00 0.00 3.549 365 atcgatcagtcagctagctag 126 21 0 47.62 57.445 17.46 17.46 0.00 3.555 366 tcgcctagctagcatcga 424 18 0 55.56 58.427 22.91 21.73 0.00 3.573 367 tactagctagcatcgatcgatca 141 23 0 43.48 59.424 24.35 14.31 32.26 3.576 368 atgatcgactagctagctacgat 634 23 0 43.48 59.422 34.20 34.20 39.30 3.578 369 gctagcatcgatcgcgcag 387 19 0 63.16 62.586 20.81 17.21 37.79 3.586 370 atcgatgatgatcagctagct 531 21 0 42.86 57.405 16.77 15.18 32.11 3.595 371 gctagcatcgatcgcgca 387 18 0 61.11 61.605 25.40 14.07 37.79 3.605 372 gatcgactagctagctacgatc 632 22 0 50.00 58.390 37.23 37.23 42.12 3.610 373 gactagctagctacgatcgatc 628 22 0 50.00 58.390 16.29 16.29 0.00 3.610 374 ctagctagctacgatcgatcag 626 22 0 50.00 58.387 19.56 13.89 0.00 3.613 375 ctagctacgatcgatcagctag 622 22 0 50.00 58.387 36.80 36.80 40.09 3.613 376 ctacgatcgatcagctagctag 581 22 0 50.00 58.387 17.46 17.46 0.00 3.613 377 cgtatcagtcagctagctagt 484 21 0 47.62 57.384 22.18 6.94 0.00 3.616 378 tcgcgtatcagtcagctag 487 19 0 52.63 57.370 6.10 0.00 0.00 3.630 379 gctagcatcgatgatgatcag 537 21 0 47.62 57.358 10.39 2.54 42.87 3.642 380 ctagcatcgatgatgatcagc 536 21 0 47.62 57.358 7.32 0.00 42.87 3.642 381 gcatcgatgatgatcagctag 533 21 0 47.62 57.358 29.80 0.00 42.87 3.642 382 catcgatgatgatcagctagc 532 21 0 47.62 57.358 21.68 7.15 42.87 3.642 383 gctagcatgatgatcgatcag 290 21 0 47.62 57.358 30.77 19.47 45.35 3.642 384 atgtagcatcgatcagtagct 327 21 0 42.86 57.344 10.18 10.18 36.12 3.656 385 tagccgcacatcgatcgatca 100 21 0 52.38 62.663 24.95 12.90 42.05 3.663 386 ctagctagcatcgtcagct 672 19 0 52.63 57.336 8.73 5.75 0.00 3.664 387 agctagcatcgtcagctag 670 19 0 52.63 57.336 21.54 21.54 33.70 3.664 388 ctagcatcgtcagctagct 668 19 0 52.63 57.336 16.85 15.18 0.00 3.664 389 agcatcgtcagctagctag 666 19 0 52.63 57.336 17.46 17.46 0.00 3.664 390 catcgcctagctagcatcgatc 426 22 0 54.55 61.702 25.81 7.21 32.23 3.702 391 tcgcgtatcagtcagctagcta 487 22 0 50.00 61.730 4.08 0.00 0.00 3.730 392 ctagctagctacgatcgatca 626 21 0 47.62 57.269 24.95 6.39 0.00 3.731 393 tagctagctacgatcgatcag 625 21 0 47.62 57.269 27.80 13.89 0.00 3.731 394 ctagctacgatcgatcagcta 622 21 0 47.62 57.269 32.87 31.34 33.83 3.731 395 tagctacgatcgatcagctag 621 21 0 47.62 57.269 32.79 23.26 33.83 3.731 396 ctacgatcgatcagctagcta 581 21 0 47.62 57.269 16.25 2.83 0.00 3.731 397 tacgatcgatcagctagctag 580 21 0 47.62 57.269 17.46 17.46 0.00 3.731 398 ctactagctagcatcgatcga 142 21 0 47.62 57.269 22.52 22.52 0.00 3.731 399 gatcgactagctagctacgat 632 21 0 47.62 57.267 34.20 34.20 39.30 3.733 400 atcgactagctagctacgatc 631 21 0 47.62 57.267 30.99 22.62 35.52 3.733 401 gactagctagctacgatcgat 628 21 0 47.62 57.267 15.49 1.14 0.00 3.733 402 actagctagctacgatcgatc 627 21 0 47.62 57.267 24.00 16.29 0.00 3.733 403 tgtagcatcgatcagtagctagc 326 23 0 47.83 60.734 9.53 7.18 35.96 3.734 404 gatgatcgatcagtcagctagct 282 23 0 47.83 60.736 26.17 15.18 41.24 3.736 405 tcgatcgatcagtcagctagct 129 22 0 50.00 61.737 19.14 15.18 0.00 3.737 406 agcatcgatcgcgcagaga 384 19 0 57.89 62.740 25.40 3.37 43.32 3.740 407 tcgcctagctagcatcgatcg 424 21 0 57.14 62.770 21.96 13.37 0.00 3.770 408 cgcctagctagcatcgatcga 423 21 0 57.14 62.770 22.52 22.52 0.00 3.770 409 catgcatgcatcgatcgat 461 19 0 47.37 57.226 25.37 25.37 34.54 3.774 410 cgatgatgatcagctagctagt 529 22 0 45.45 58.219 22.18 7.88 32.11 3.781 411 ctagtagcatcgatgatcagct 215 22 0 45.45 58.219 13.24 9.66 0.00 3.781 412 agtagcatcgatgatcagctag 213 22 0 45.45 58.219 20.75 3.33 29.76 3.781 413 tcgatcagtcagctagctaaa 277 21 0 42.86 57.216 0.00 0.00 0.00 3.784 414 agatgatgatcagctagctagc 167 22 0 45.45 58.203 34.67 34.67 45.14 3.797 415 agcatcgatgatcagctagctag 210 23 0 47.83 60.799 16.83 17.46 0.00 3.799 416 agctagctacgatcgatcagct 624 22 0 50.00 61.804 32.07 32.07 35.03 3.804 417 tcgatcgatgatagtagctagct 451 23 0 43.48 59.170 23.74 23.74 0.00 3.830 418 gatcgatcagtcagctagctaa 279 22 0 45.45 58.159 21.11 0.00 0.00 3.841 419 tgatgatcgatcagtcagctag 283 22 0 45.45 58.155 28.37 0.00 41.24 3.845 420 agcatcgcgtatcagtcagct 491 21 0 52.38 62.876 2.31 0.00 0.00 3.876 421 tagctagcatgatcgactagcta 642 23 0 43.48 59.102 33.45 31.70 42.99 3.898 422 tagcatgatcgactagctagcta 638 23 0 43.48 59.102 25.55 23.56 33.71 3.898 423 tagcatcgatcagtagctagcta 324 23 0 43.48 59.102 23.52 19.77 34.70 3.898 424 gatcgactagctagctacgatcg 632 23 0 52.17 60.902 37.10 32.84 41.10 3.902 425 cgactagctagctacgatcgatc 629 23 0 52.17 60.902 25.89 16.29 0.00 3.902 426 actagctagcatcgatgatga 541 21 0 42.86 57.067 21.52 0.00 42.87 3.933 427 agtagctagcatgatgatcga 294 21 0 42.86 57.067 0.00 0.00 0.00 3.933 428 tagtagcatcgatgatcagct 214 21 0 42.86 57.067 13.24 9.66 0.00 3.933 429 agtagcatcgatgatcagcta 213 21 0 42.86 57.067 20.75 12.45 0.00 3.933 430 ctagcatactagcatcgatcga 348 22 0 45.45 58.044 22.52 22.52 40.35 3.956 431 agtcagctagctagatcgc 602 19 0 52.63 57.034 12.77 5.13 0.00 3.966 432 gatgtagcatcgatcagtagc 328 21 0 47.62 57.028 0.00 0.00 31.80 3.972 433 cagctagctagatcgccg 599 18 0 61.11 58.018 13.18 0.00 0.00 3.982 434 tgatgatcgatcagtcagctagc 283 23 0 47.83 60.983 28.37 14.31 41.24 3.983 435 tgatgatcgatcagtcagcta 283 21 0 42.86 57.000 28.37 13.35 41.24 4.000 436 atcgatgatgatcagctagctagt 531 24 0 41.67 59.995 22.18 7.88 32.11 4.005 437 tcgatgatgatcagctagctag 530 22 0 45.45 57.958 17.46 17.46 32.11 4.042 438 gctagtagcatcgatgatcagct 216 23 0 47.83 61.046 22.39 18.22 44.86 4.046 439 agtagcatcgatgatcagctagc 213 23 0 47.83 61.046 15.66 8.41 29.76 4.046 440 gtagcatcgatgatcagctagct 212 23 0 47.83 61.046 20.84 20.84 29.76 4.046 441 ctagctagcatgatcgactagcta 643 24 0 45.83 60.058 32.02 30.68 42.99 4.058 442 tagctagcatgatcgactagctag 642 24 0 45.83 60.058 31.95 25.48 42.99 4.058 443 ctagcatgatcgactagctagcta 639 24 0 45.83 60.058 25.55 23.56 40.71 4.058 444 cgcagagatgatagtagctagcta 373 24 0 45.83 60.058 23.52 19.77 0.00 4.058 445 tagcatcgatcagtagctagctag 324 24 0 45.83 60.058 24.35 17.46 34.70 4.058 446 tcgcgcagagatgatagtagct 376 22 0 50.00 62.058 5.16 0.00 0.00 4.058 447 gtagctagcatgatgatcgatca 293 23 0 43.48 58.931 28.62 28.62 45.96 4.069 448 cgatcgatcagtcagctagctag 128 23 0 52.17 61.091 17.46 17.46 0.00 4.091 449 tgtagcatcgatcagtagctag 326 22 0 45.45 57.898 11.52 0.00 35.96 4.102 450 tagtcagctagctagatcgccg 603 22 0 54.55 62.109 12.77 2.27 0.00 4.109 451 gcatcgcctagctagcat 427 18 0 55.56 57.890 6.22 0.00 0.00 4.110 452 gtagctagcatgatgatcgatcag 293 24 0 45.83 59.882 30.77 19.47 45.35 4.118 453 gctagcatcgcgtatcagtcag 494 22 0 54.55 62.128 11.13 0.00 0.00 4.128 454 ctagcatcgcgtatcagtcagc 493 22 0 54.55 62.128 0.00 0.00 0.00 4.128 455 gcatcgcgtatcagtcagctag 490 22 0 54.55 62.128 0.00 0.00 0.00 4.128 456 catcgcgtatcagtcagctagc 489 22 0 54.55 62.128 7.15 7.15 0.00 4.128 457 ctagcatgatgatcgatcagtca 289 23 0 43.48 58.866 28.37 27.49 45.35 4.134 458 tagcatgatgatcgatcagtcag 288 23 0 43.48 58.866 23.86 19.51 45.35 4.134 459 catgatgatcgatcagtcagcta 285 23 0 43.48 58.866 22.18 9.87 45.35 4.134 460 ctagctagctacgatcgatcagc 626 23 0 52.17 61.151 24.24 24.24 0.00 4.151 461 gctagctacgatcgatcagctag 623 23 0 52.17 61.151 40.11 40.11 43.70 4.151 462 gctacgatcgatcagctagctag 582 23 0 52.17 61.151 24.35 17.11 0.00 4.151 463 cctagctagcatcgatcgatcag 421 23 0 52.17 61.158 19.62 17.44 32.26 4.158 464 ctactagctagcatcgatcgatc 142 23 0 47.83 58.829 20.96 16.29 32.26 4.171 465 atgatcgatcagtcagctagctaa 281 24 0 41.67 60.177 30.77 5.30 41.24 4.177 466 ctagcatgatgatcgatcagtcag 289 24 0 45.83 59.822 23.86 19.51 45.35 4.178 467 catgatgatcgatcagtcagctag 285 24 0 45.83 59.822 22.18 14.49 45.35 4.178 468 gatcgatcagtcagctagctaaa 279 23 0 43.48 58.807 21.11 0.00 0.00 4.193 469 tagcatgatgatcgatcagtca 288 22 0 40.91 57.803 28.37 27.49 45.35 4.197 470 tcgatcgatgatagtagctagc 451 22 0 45.45 57.783 18.63 7.15 0.00 4.217 471 tactagctagcatcgatcgatc 141 22 0 45.45 57.783 20.96 16.29 32.26 4.217 472 cgatcgatgatagtagctagct 450 22 0 45.45 57.777 23.74 23.74 0.00 4.223 473 ctactagctagcatcgatcgat 142 22 0 45.45 57.777 25.37 25.37 35.13 4.223 474 actagctagcatcgatgatgatca 541 24 0 41.67 60.238 21.52 3.85 42.87 4.238 475 agtagctagcatgatgatcgatca 294 24 0 41.67 60.238 28.62 28.62 45.96 4.238 476 tagctagcatgatgatcgatcagt 292 24 0 41.67 60.238 12.39 3.56 45.35 4.238 477 ctagctagtagcccagctagcta 688 23 0 52.17 61.253 24.83 23.65 42.96 4.253 478 tagctagtagcccagctagctag 687 23 0 52.17 61.253 24.76 19.33 44.03 4.253 479 atcgatcagtcagctagctaaa 278 22 0 40.91 57.736 0.00 0.00 0.00 4.264 480 tcgatcagtcagctagctaaat 277 22 0 40.91 57.736 2.32 0.00 0.00 4.264 481 tagctagcatcgatcgcg 389 18 0 55.56 57.717 19.30 4.04 39.35 4.283 482 atcgactagctagctacgatcga 631 23 0 47.83 61.293 29.80 29.80 35.52 4.293 483 tcgactagctagctacgatcgat 630 23 0 47.83 61.293 28.17 11.90 0.00 4.293 484 tagcatgatcgactagctagctac 638 24 0 45.83 60.297 13.83 3.08 33.71 4.297 485 gtagcatcgatcagtagctagcta 325 24 0 45.83 60.297 23.52 19.77 35.96 4.297 486 atcgcgtatcagtcagctagct 488 22 0 50.00 62.317 16.77 15.18 0.00 4.317 487 ctagctagcatcgatgatgatca 540 23 0 43.48 58.678 19.69 3.85 42.87 4.322 488 tagctagcatcgatgatgatcag 539 23 0 43.48 58.678 19.30 2.54 42.87 4.322 489 ctagcatcgatgatgatcagcta 536 23 0 43.48 58.678 18.28 12.21 42.87 4.322 490 tagcatcgatgatgatcagctag 535 23 0 43.48 58.678 18.18 3.22 42.87 4.322 491 catcgatgatgatcagctagcta 532 23 0 43.48 58.678 21.68 0.00 42.87 4.322 492 tagctagcatgatgatcgatcag 292 23 0 43.48 58.678 30.77 19.47 45.35 4.322 493 actagctagcatcgatgatgatc 541 23 0 43.48 58.677 21.52 1.03 42.87 4.323 494 agtagctagcatgatgatcgatc 294 23 0 43.48 58.677 19.32 19.32 0.00 4.323 495 gctacgatcgatcagctagtcag 619 23 0 52.17 61.332 24.45 8.63 0.00 4.332 496 ctactagctagcatcgatcgatca 142 24 0 45.83 60.356 24.35 14.31 32.26 4.356 497 tactagctagcatcgatcgatcag 141 24 0 45.83 60.356 21.52 17.44 32.26 4.356 498 atgatcgactagctagctacgatc 634 24 0 45.83 60.356 39.87 39.87 44.89 4.356 499 ctagctagcatcgatgatgatcag 540 24 0 45.83 59.644 12.04 4.02 42.87 4.356 500 ctagcatcgatgatgatcagctag 536 24 0 45.83 59.644 22.97 20.45 42.87 4.356 501 catcgatgatgatcagctagctag 532 24 0 45.83 59.644 21.68 17.46 42.87 4.356 502 tgatcgatcagtcagctagctaaa 280 24 0 41.67 60.358 26.87 0.00 32.65 4.358 503 cgctacgatcgatcagct 583 18 0 55.56 57.634 26.08 13.79 0.00 4.366 504 agctagcatcgatcgatcagct 418 22 0 50.00 62.388 29.94 29.94 38.16 4.388 505 agcatcgatcgatcagctagct 414 22 0 50.00 62.388 30.67 27.68 34.69 4.388 506 atgatgatcgatcagtcagctag 284 23 0 43.48 58.612 29.63 0.00 45.35 4.388 507 gcagagatgatagtagctagct 372 22 0 45.45 57.608 23.74 23.74 0.00 4.392 508 cgcgcagagatgatagtagctag 375 23 0 52.17 61.393 0.00 0.00 0.00 4.393 509 tagctagcatcgatgatgatca 539 22 0 40.91 57.602 19.69 2.20 42.87 4.398 510 tagcatcgatgatgatcagcta 535 22 0 40.91 57.602 19.82 11.98 42.87 4.398 511 tagctagcatgatgatcgatca 292 22 0 40.91 57.602 28.62 28.62 45.96 4.398 512 actagctagcatcgatgatgat 541 22 0 40.91 57.595 21.52 3.02 42.87 4.405 513 agtagctagcatgatgatcgat 294 22 0 40.91 57.595 0.00 0.00 0.00 4.405 514 atcgatcgatgatagtagctagct 452 24 0 41.67 59.573 29.65 23.74 33.56 4.427 515 atgatgatcgatcagtcagcta 284 22 0 40.91 57.531 29.63 16.50 45.35 4.469 516 tcgatcgatcagtcagctagcta 129 23 0 47.83 61.487 19.14 0.00 0.00 4.487 517 ctagcatactagcatcgatcgat 348 23 0 43.48 58.501 25.37 25.37 40.35 4.499 518 gctagtagcccagctagc 685 18 0 61.11 57.495 11.96 10.22 42.96 4.505 519 gatgatcgatcagtcagctagcta 282 24 0 45.83 60.536 26.17 5.54 41.24 4.536 520 tagctagctacgatcgatcagct 625 23 0 47.83 61.551 32.07 32.07 35.03 4.551 521 agctagctacgatcgatcagcta 624 23 0 47.83 61.551 32.87 31.34 33.83 4.551 522 tcgatgatgatcagctagctagta 530 24 0 41.67 59.449 0.94 0.00 32.11 4.551 523 agctagcatcgcgtatcagtca 495 22 0 50.00 62.569 19.30 0.00 0.00 4.569 524 tagcatcgcgtatcagtcagct 492 22 0 50.00 62.569 5.62 5.62 0.00 4.569 525 agcatcgcgtatcagtcagcta 491 22 0 50.00 62.569 2.31 0.00 0.00 4.569 526 tagcatactagcatcgatcgat 347 22 0 40.91 57.423 25.37 25.37 37.20 4.577 527 atcgatgatgatcagctagctag 531 23 0 43.48 58.422 17.46 17.46 32.11 4.578 528 agcatcgatcgatcagtcagct 133 22 0 50.00 62.580 27.16 26.03 32.26 4.580 529 catgatcgactagctagctacgat 635 24 0 45.83 60.593 34.20 34.20 39.30 4.593 530 tagcatcgatgatcagctagctag 211 24 0 45.83 60.596 22.08 17.46 0.00 4.596 531 agctagcatcgatgatgatcagc 538 23 0 47.83 61.603 19.30 16.06 42.87 4.603 532 gctagcatcgatgatgatcagct 537 23 0 47.83 61.603 17.59 10.14 42.87 4.603 533 agcatcgatgatgatcagctagc 534 23 0 47.83 61.603 15.88 10.46 42.87 4.603 534 gcatcgatgatgatcagctagct 533 23 0 47.83 61.603 29.80 15.18 42.87 4.603 535 cgtatcagtcagctagctagtag 484 23 0 47.83 58.387 5.61 0.55 0.00 4.613 536 gatgtagcatcgatcagtagcta 328 23 0 43.48 58.368 10.63 8.84 34.70 4.632 537 gcatgatcgactagctagctacg 636 23 0 52.17 61.633 21.95 21.95 0.00 4.633 538 ctagctagcatcgcgtatcagtc 497 23 0 52.17 61.633 8.73 0.00 0.00 4.633 539 atgtagcatcgatcagtagctag 327 23 0 43.48 58.364 11.52 0.00 35.96 4.636 540 gcgcagagatgatagtagctagc 374 23 0 52.17 61.644 7.15 7.15 0.00 4.644 541 gatgtagcatcgatcagtagctag 328 24 0 45.83 59.349 11.52 0.00 35.96 4.651 542 gctactagctagatgatgatcagc 177 24 0 45.83 59.343 15.34 8.75 41.51 4.657 543 atcgatgatgatcagctagcta 531 22 0 40.91 57.329 7.29 0.00 32.11 4.671 544 cgtatcagtcagctagctagta 484 22 0 45.45 57.310 2.33 0.00 0.00 4.690 545 ctagctagcatcgatcgatcagc 420 23 0 52.17 61.697 27.48 27.48 32.26 4.697 546 gctagcatcgatcgatcagctag 417 23 0 52.17 61.697 41.07 41.07 46.89 4.697 547 ctagcatcgatcgatcagctagc 416 23 0 52.17 61.697 38.64 31.64 43.05 4.697 548 gcatcgatcgatcagctagctag 413 23 0 52.17 61.697 26.76 17.46 32.26 4.697 549 catcgatcgatcagctagctagc 412 23 0 52.17 61.697 34.67 34.67 40.69 4.697 550 gtagctagcatgatgatcgatc 293 22 0 45.45 57.284 19.32 19.32 0.00 4.716 551 atcgcctagctagcatcg 425 18 0 55.56 57.277 17.52 17.52 0.00 4.723 552 cgcctagctagcatcgat 423 18 0 55.56 57.277 18.32 0.00 0.00 4.723 553 atgtagcatcgatcagtagcta 327 22 0 40.91 57.270 10.63 8.84 34.70 4.730 554 agctacgatcgatcagctagtca 620 23 0 47.83 61.737 28.83 4.73 31.57 4.737 555 atcgatcgatgatagtagctagc 452 23 0 43.48 58.251 29.65 7.15 33.56 4.749 556 gatcgatcagtcagctagctaaat 279 24 0 41.67 59.219 21.11 0.00 0.00 4.781 557 ctagcatgatgatcgatcagtc 289 22 0 45.45 57.215 25.60 21.92 45.35 4.785 558 atcgatcagtcagctagctaaat 278 23 0 39.13 58.215 2.32 0.00 0.00 4.785 559 agcatgatgatcgatcagtcagc 287 23 0 47.83 61.788 28.75 27.74 45.35 4.788 560 gcatgatgatcgatcagtcagct 286 23 0 47.83 61.788 28.73 19.78 45.35 4.788 561 tcgcgcagagatgatagtagcta 376 23 0 47.83 61.796 5.16 0.00 0.00 4.796 562 gctagtagcatcgatgatcagcta 216 24 0 45.83 60.834 22.39 18.36 44.86 4.834 563 tagtagcatcgatgatcagctagc 214 24 0 45.83 60.834 13.54 8.41 29.76 4.834 564 gtagcatcgatgatcagctagcta 212 24 0 45.83 60.834 20.84 18.43 29.76 4.834 565 gctagcatcgatcgatcagtcag 136 23 0 52.17 61.878 16.51 12.53 32.26 4.878 566 ctagcatcgatcgatcagtcagc 135 23 0 52.17 61.878 18.38 17.16 32.26 4.878 567 gcatcgatcgatcagtcagctag 132 23 0 52.17 61.878 21.92 10.82 32.26 4.878 568 catcgatcgatcagtcagctagc 131 23 0 52.17 61.878 17.10 7.15 32.26 4.878 569 gactagctagctacgatcgatcag 628 24 0 50.00 60.882 18.86 13.89 0.00 4.882 570 ctagctacgatcgatcagctagtc 622 24 0 50.00 60.882 38.29 23.73 40.64 4.882 571 ctactagctagcatcgatgatga 543 23 0 43.48 58.114 10.08 0.00 42.87 4.886 572 cgatgatgatcagctagctagta 529 23 0 43.48 58.114 5.39 0.00 32.11 4.886 573 ctagtagctagcatgatgatcga 296 23 0 43.48 58.114 0.00 0.00 0.00 4.886 574 ctagtagcatcgatgatcagcta 215 23 0 43.48 58.114 13.62 10.55 0.00 4.886 575 tagtagcatcgatgatcagctag 214 23 0 43.48 58.114 13.23 3.33 29.76 4.886 576 cgatgatgatcagctagctagtag 529 24 0 45.83 59.110 5.39 0.55 32.11 4.890 577 ctagtagcatcgatgatcagctag 215 24 0 45.83 59.110 25.99 23.79 29.76 4.890 578 tagatgatgatcagctagctagc 168 23 0 43.48 58.097 34.67 34.67 45.14 4.903 579 tcgatcgatgatagtagctagcta 451 24 0 41.67 59.031 23.52 19.77 0.00 4.969 580 cgctacgatcgatcagctagct 583 22 0 54.55 62.971 26.39 15.18 0.00 4.971 581 agcatcgcgtatcagtca 491 18 0 50.00 57.028 0.00 0.00 0.00 4.972 582 ctagctagcatcgatgatgatc 540 22 0 45.45 57.019 21.07 9.90 42.87 4.981 583 tactagctagcatcgatgatga 542 22 0 40.91 57.005 21.52 0.00 42.87 4.995 584 tagtagctagcatgatgatcga 295 22 0 40.91 57.005 0.00 0.00 0.00 4.995 585 tagtagcatcgatgatcagcta 214 22 0 40.91 57.005 14.66 10.27 0.00 4.995 586 atcgcgtatcagtcagctagcta 488 23 0 47.83 62.044 4.08 0.00 0.00 5.044 587 tcgatcgatgatagtagctagctag 451 25 0 44.00 59.949 24.35 17.46 0.00 5.051 588 atcgatcgatcagtcagctagct 130 23 0 47.83 62.053 25.37 15.18 0.00 5.053 589 gcgtatcagtcagctagctagtag 485 24 0 50.00 61.062 5.57 0.55 0.00 5.062 590 tactagctagcatcgatgatgatca 542 25 0 40.00 60.062 21.52 3.85 42.87 5.062 591 tagtagctagcatgatgatcgatca 295 25 0 40.00 60.062 28.62 28.62 45.96 5.062 592 atgtagcatcgatcagtagctagc 327 24 0 45.83 61.072 9.53 7.18 35.96 5.072 593 tagctagcatcgatcgatcagct 419 23 0 47.83 62.111 32.90 32.90 38.16 5.111 594 agctagcatcgatcgatcagcta 418 23 0 47.83 62.111 30.90 29.54 36.80 5.111 595 tagcatcgatcgatcagctagct 415 23 0 47.83 62.111 34.03 31.37 36.80 5.111 596 agcatcgatcgatcagctagcta 414 23 0 47.83 62.111 30.67 5.03 34.69 5.111 597 atcgatgatgatcagctagctagta 531 25 0 40.00 59.828 0.94 0.00 32.11 5.172 598 tagctagcatcgcgtatcagtca 496 23 0 47.83 62.287 19.30 0.00 0.00 5.287 599 tagcatcgcgtatcagtcagcta 492 23 0 47.83 62.287 8.22 6.29 0.00 5.287 600 agctagcatcgatcgatcagtca 137 23 0 47.83 62.296 18.50 8.61 32.26 5.296 601 tagcatcgatcgatcagtcagct 134 23 0 47.83 62.296 30.27 30.27 32.26 5.296 602 agcatcgatcgatcagtcagcta 133 23 0 47.83 62.296 27.34 5.68 32.26 5.296 603 cgatcgatgatagtagctagctag 450 24 0 45.83 58.703 24.35 17.46 0.00 5.297 604 cgatcgatgatagtagctagcta 450 23 0 43.48 57.691 23.52 19.77 0.00 5.309 605 agctagcatgatgatcgatcagtc 291 24 0 45.83 61.313 25.60 21.92 45.35 5.313 606 atgatgatcgatcagtcagctagc 284 24 0 45.83 61.313 29.63 15.68 45.35 5.313 607 tagctagctacgatcgatcagcta 625 24 0 45.83 61.318 32.87 31.34 33.83 5.318 608 tcgatcagtcagctagctaaatag 277 24 0 41.67 58.679 3.40 0.00 0.00 5.321 609 tcgatcagtcagctagctaaata 277 23 0 39.13 57.650 0.00 0.00 0.00 5.350 610 tcgatgatgatcagctagctagtag 530 25 0 44.00 60.352 5.57 0.55 32.11 5.352 611 tagctagcatcgatgatgatcagc 539 24 0 45.83 61.370 19.30 16.06 42.87 5.370 612 gctagcatcgatgatgatcagcta 537 24 0 45.83 61.370 17.59 10.60 42.87 5.370 613 tagcatcgatgatgatcagctagc 535 24 0 45.83 61.370 18.18 11.26 42.87 5.370 614 gcatcgatgatgatcagctagcta 533 24 0 45.83 61.370 29.80 0.00 42.87 5.370 615 ctagctagcatcgatcgatcagtc 139 24 0 50.00 61.409 15.70 11.46 32.26 5.409 616 gcagagatgatagtagctagctag 372 24 0 45.83 58.562 24.35 17.46 0.00 5.438 617 tactagctagcatcgatgatgatc 542 24 0 41.67 58.557 21.52 1.03 42.87 5.443 618 tagtagctagcatgatgatcgatc 295 24 0 41.67 58.557 19.32 19.32 0.00 5.443 619 ctactagctagcatcgatgatgat 543 24 0 41.67 58.554 0.00 0.00 42.87 5.446 620 ctagtagctagcatgatgatcgat 296 24 0 41.67 58.554 0.00 0.00 0.00 5.446 621 gcagagatgatagtagctagcta 372 23 0 43.48 57.527 23.52 19.77 0.00 5.473 622 tactagctagcatcgatgatgat 542 23 0 39.13 57.514 21.52 0.00 42.87 5.486 623 tagtagctagcatgatgatcgat 295 23 0 39.13 57.514 0.00 0.00 0.00 5.486 624 tagctacgatcgatcagctagtca 621 24 0 45.83 61.497 32.11 20.31 33.83 5.497 625 ctactagctagcatcgatgatgatc 543 25 0 44.00 59.494 0.00 0.00 42.87 5.506 626 ctagtagctagcatgatgatcgatc 296 25 0 44.00 59.494 19.32 19.32 0.00 5.506 627 gctagcatgatgatcgatcagtca 290 24 0 45.83 61.547 28.37 27.49 45.35 5.547 628 tagcatgatgatcgatcagtcagc 288 24 0 45.83 61.547 28.75 27.74 45.35 5.547 629 gcatgatgatcgatcagtcagcta 286 24 0 45.83 61.547 28.73 17.41 45.35 5.547 630 tcgcgtatcagtcagctagctag 487 23 0 52.17 62.571 17.46 17.46 0.00 5.571 631 atcgatcgatgatagtagctagcta 452 25 0 40.00 59.423 29.65 19.77 33.56 5.577 632 atcgactagctagctacgatcgat 631 24 0 45.83 61.611 30.75 30.75 35.52 5.611 633 cgatcagtcagctagctaaatag 276 23 0 43.48 57.339 0.00 0.00 0.00 5.661 634 cgctacgatcgatcagctagcta 583 23 0 52.17 62.677 26.39 12.89 0.00 5.677 635 atgatcgatcagtcagctagctaaa 281 25 0 40.00 60.702 30.77 4.66 41.24 5.702 636 tgatcgatcagtcagctagctaaat 280 25 0 40.00 60.702 26.87 0.00 32.65 5.702 637 atcgatcgatcagtcagctagcta 130 24 0 45.83 61.800 25.37 0.00 0.00 5.800 638 cgcgtatcagtcagctagctagt 486 23 0 52.17 62.816 22.18 6.94 0.00 5.816 639 tagctagcatcgatcgatcagcta 419 24 0 45.83 61.857 34.69 33.24 36.80 5.857 640 tagcatcgatcgatcagctagcta 415 24 0 45.83 61.857 34.03 30.67 36.80 5.857 641 atcgatcagtcagctagctaaata 278 24 0 37.50 58.112 0.00 0.00 0.00 5.888 642 gatcgatcagtcagctagctaaata 279 25 0 40.00 59.084 21.11 0.00 0.00 5.916 643 atcgatcagtcagctagctaaatag 278 25 0 40.00 59.082 3.40 0.00 0.00 5.918 644 cgcagagatgatagtagctagctag 373 25 0 48.00 60.920 24.35 17.46 0.00 5.920 645 ctagtcagctagctagatcgccg 604 23 0 56.52 62.926 11.71 0.00 39.03 5.926 646 gcctagctagcatcgatcgatca 422 23 0 52.17 62.955 24.35 14.31 32.26 5.955 647 tgtagcatcgatcagtagctagct 326 24 0 45.83 61.988 23.74 23.74 35.96 5.988 648 tagctagcatcgatcgatcagtca 138 24 0 45.83 62.034 18.50 10.55 32.26 6.034 649 tagcatcgatcgatcagtcagcta 134 24 0 45.83 62.034 32.66 30.74 32.26 6.034 650 actagctagcatcgatcgatcagt 140 24 0 45.83 62.039 21.52 9.07 32.26 6.039 651 gatcgatcagtcagctagctaaatag 279 26 0 42.31 59.960 21.11 0.00 0.00 6.040 652 gatgatcgatcagtcagctagctaa 282 25 0 44.00 61.041 26.17 5.19 41.24 6.041 653 agatgatgatcagctagctagtag 232 24 0 41.67 57.930 6.28 0.55 45.14 6.070 654 tagctagcatgatgatcgatcagtc 292 25 0 44.00 61.099 25.60 21.92 45.35 6.099 655 actagctagcatcgatgatgatcag 541 25 0 44.00 61.101 21.52 10.83 42.87 6.101 656 catcgatgatgatcagctagctagt 532 25 0 44.00 61.101 21.68 7.88 42.87 6.101 657 agtagctagcatgatgatcgatcag 294 25 0 44.00 61.101 30.77 19.47 45.35 6.101 658 ctagctagtagcccagctagctag 688 24 0 54.17 62.101 29.36 29.36 46.15 6.101 659 gatcgactagctagctacgatcga 632 24 0 50.00 62.118 37.10 35.19 41.10 6.118 660 tcgactagctagctacgatcgatc 630 24 0 50.00 62.118 28.17 15.96 0.00 6.118 661 ctagcatgatcgactagctagctac 639 25 0 48.00 61.145 17.67 3.08 40.71 6.145 662 gtagcatcgatcagtagctagctag 325 25 0 48.00 61.145 24.35 17.46 35.96 6.145 663 ctactagctagcatcgatcgatcag 142 25 0 48.00 61.197 19.62 17.44 32.26 6.197 664 tgatgatcgatcagtcagctagct 283 24 0 45.83 62.232 28.37 15.18 41.24 6.232 665 atcgatcgatgatagtagctagctag 452 26 0 42.31 60.291 29.65 17.46 33.56 6.291 666 agtagcatcgatgatcagctagct 213 24 0 45.83 62.294 18.27 15.68 29.76 6.294 667 tcgatcgatcagtcagctagctag 129 24 0 50.00 62.312 19.14 17.46 0.00 6.312 668 gtagctagcatgatgatcgatcagt 293 25 0 44.00 61.328 12.39 3.56 45.35 6.328 669 tgatcgactagctagctacgatcg 633 24 0 50.00 62.352 39.64 34.36 46.11 6.352 670 cgactagctagctacgatcgatca 629 24 0 50.00 62.352 25.89 8.46 0.00 6.352 671 ctagctagctacgatcgatcagct 626 24 0 50.00 62.373 32.07 32.07 35.03 6.373 672 agctagctacgatcgatcagctag 624 24 0 50.00 62.373 40.11 40.11 43.70 6.373 673 gctactagctagatgatgatcagcta 177 26 0 42.31 60.404 19.96 18.89 46.14 6.404 674 catgatcgactagctagctacgatc 635 25 0 48.00 61.421 39.87 39.87 44.89 6.421 675 tgatcgatcagtcagctagctaaata 280 26 0 38.46 60.516 26.87 0.00 32.65 6.516 676 cgcgtatcagtcagctagctagta 486 24 0 50.00 62.538 2.33 0.00 0.00 6.538 677 agctacgatcgatcagctagtcag 620 24 0 50.00 62.548 28.83 10.43 31.57 6.548 678 tcgcgcagagatgatagtagctag 376 24 0 50.00 62.603 5.16 0.00 0.00 6.603 679 actagctagctacgatcgatcagc 627 24 0 50.00 62.604 24.24 24.24 0.00 6.604 680 gctagctacgatcgatcagctagt 623 24 0 50.00 62.604 41.33 28.87 43.42 6.604 681 gctagtagcatcgatgatcagctag 216 25 0 48.00 61.656 28.66 28.05 44.86 6.656 682 ctagtagcatcgatgatcagctagc 215 25 0 48.00 61.656 26.51 16.06 29.76 6.656 683 gtagcatcgatgatcagctagctag 212 25 0 48.00 61.656 21.48 17.46 29.76 6.656 684 atcgatgatgatcagctagctagtag 531 26 0 42.31 60.681 5.57 0.55 32.11 6.681 685 tgtagcatcgatcagtagctagcta 326 25 0 44.00 61.746 23.52 19.77 35.96 6.746 686 tactagctagcatcgatcgatcagt 141 25 0 44.00 61.797 21.52 11.62 32.26 6.797 687 gcatgatcgactagctagctacga 636 24 0 50.00 62.833 29.72 29.72 34.25 6.833 688 agctagcatcgatgatgatcagct 538 24 0 45.83 62.835 20.20 19.52 42.87 6.835 689 agcatcgatgatgatcagctagct 534 24 0 45.83 62.835 15.88 14.93 42.87 6.835 690 agcatgatcgactagctagctacg 637 24 0 50.00 62.838 21.95 21.95 0.00 6.838 691 atcgcgtatcagtcagctagctag 488 24 0 50.00 62.838 17.46 17.46 0.00 6.838 692 gcgcagagatgatagtagctagct 374 24 0 50.00 62.858 23.74 23.74 0.00 6.858 693 gatgtagcatcgatcagtagctagc 328 25 0 48.00 61.879 9.53 7.18 35.96 6.879 694 ctactagctagcatcgatgatgatca 543 26 0 42.31 60.902 19.69 3.85 42.87 6.902 695 tactagctagcatcgatgatgatcag 542 26 0 42.31 60.902 21.52 10.83 42.87 6.902 696 catcgatgatgatcagctagctagta 532 26 0 42.31 60.902 21.68 0.00 42.87 6.902 697 ctagtagctagcatgatgatcgatca 296 26 0 42.31 60.902 28.62 28.62 45.96 6.902 698 tagtagctagcatgatgatcgatcag 295 26 0 42.31 60.902 30.77 19.47 45.35 6.902 699 ctagctagcatcgatcgatcagct 420 24 0 50.00 62.904 32.90 32.90 38.16 6.904 700 agctagcatcgatcgatcagctag 418 24 0 50.00 62.904 41.07 41.07 46.89 6.904 701 ctagcatcgatcgatcagctagct 416 24 0 50.00 62.904 38.64 34.74 43.05 6.904 702 agcatcgatcgatcagctagctag 414 24 0 50.00 62.904 30.67 17.43 34.69 6.904 703 catcgatcgatcagctagctagct 412 24 0 50.00 62.904 38.28 38.28 46.53 6.904 704 tgatgatcgatcagtcagctagcta 283 25 0 44.00 61.981 28.37 11.70 41.24 6.981 705 atgatcgatcagtcagctagctaaat 281 26 0 38.46 61.022 30.77 8.35 41.24 7.022 706 tagtagcatcgatgatcagctagct 214 25 0 44.00 62.040 20.53 17.69 29.76 7.040 707 agtagcatcgatgatcagctagcta 213 25 0 44.00 62.040 20.84 18.43 29.76 7.040 708 ctagctagctacgatcgatcagcta 626 25 0 48.00 62.120 32.87 31.34 33.83 7.120 709 tagctagctacgatcgatcagctag 625 25 0 48.00 62.120 40.11 40.11 43.70 7.120 710 tagatgatgatcagctagctagtag 233 25 0 40.00 57.842 20.63 0.55 45.14 7.158 711 ctactagctagatgatgatcagcta 176 25 0 40.00 57.842 19.96 18.89 45.14 7.158 712 ctagctagcatcgatgatgatcagc 540 25 0 48.00 62.165 17.45 16.06 42.87 7.165 713 gctagcatcgatgatgatcagctag 537 25 0 48.00 62.165 25.58 25.58 45.08 7.165 714 ctagcatcgatgatgatcagctagc 536 25 0 48.00 62.165 23.55 16.25 42.87 7.165 715 gcatcgatgatgatcagctagctag 533 25 0 48.00 62.165 29.80 17.46 42.87 7.165 716 atgtagcatcgatcagtagctagct 327 25 0 44.00 62.272 23.74 23.74 35.96 7.272 717 ctagctacgatcgatcagctagtca 622 25 0 48.00 62.290 38.29 25.27 40.64 7.290 718 tagctacgatcgatcagctagtcag 621 25 0 48.00 62.290 32.11 12.71 33.83 7.290 719 gctagcatgatgatcgatcagtcag 290 25 0 48.00 62.333 23.86 19.51 45.35 7.333 720 ctagcatgatgatcgatcagtcagc 289 25 0 48.00 62.333 28.75 27.74 45.35 7.333 721 gcatgatgatcgatcagtcagctag 286 25 0 48.00 62.333 28.73 22.77 45.35 7.333 722 catgatgatcgatcagtcagctagc 285 25 0 48.00 62.333 22.18 8.99 45.35 7.333 723 gatcgactagctagctacgatcgat 632 25 0 48.00 62.391 35.29 33.15 41.10 7.391 724 atcgactagctagctacgatcgatc 631 25 0 48.00 62.391 30.75 18.00 35.52 7.391 725 tgatgatgatagtagatgatgatcagc 245 27 0 37.04 59.533 0.00 0.00 37.84 7.467 726 atgatgatcgatcagtcagctagct 284 25 0 44.00 62.507 29.63 17.88 45.35 7.507 727 gatgatcgatcagtcagctagctaaa 282 26 0 42.31 61.507 26.17 4.66 41.24 7.507 728 tagctagcatcgatgatgatcagct 539 25 0 44.00 62.561 23.67 23.67 42.87 7.561 729 agctagcatcgatgatgatcagcta 538 25 0 44.00 62.561 20.66 12.75 42.87 7.561 730 tagcatcgatgatgatcagctagct 535 25 0 44.00 62.561 20.21 20.21 42.87 7.561 731 agcatcgatgatgatcagctagcta 534 25 0 44.00 62.561 16.31 0.00 42.87 7.561 732 tagcatgatcgactagctagctacg 638 25 0 48.00 62.570 21.95 21.95 33.71 7.570 733 atcgatcgatcagtcagctagctag 130 25 0 48.00 62.579 25.37 17.46 0.00 7.579 734 gcgcagagatgatagtagctagcta 374 25 0 48.00 62.587 23.52 19.77 0.00 7.587 735 atgatcgactagctagctacgatcg 634 25 0 48.00 62.616 39.64 34.36 46.11 7.616 736 ctagctagcatcgatcgatcagcta 420 25 0 48.00 62.632 33.46 32.30 36.80 7.632 737 tagctagcatcgatcgatcagctag 419 25 0 48.00 62.632 37.54 37.54 46.89 7.632 738 ctagcatcgatcgatcagctagcta 416 25 0 48.00 62.632 38.64 34.30 43.05 7.632 739 tagcatcgatcgatcagctagctag 415 25 0 48.00 62.632 34.03 19.78 36.80 7.632 740 catcgatcgatcagctagctagcta 412 25 0 48.00 62.632 29.87 23.56 45.24 7.632 741 agctagcatgatgatcgatcagtca 291 25 0 44.00 62.733 28.37 27.49 45.35 7.733 742 tagcatgatgatcgatcagtcagct 288 25 0 44.00 62.733 32.31 32.31 45.35 7.733 743 agcatgatgatcgatcagtcagcta 287 25 0 44.00 62.733 30.60 19.08 45.35 7.733 744 ctagctagcatcgatcgatcagtca 139 25 0 48.00 62.800 18.50 11.39 32.26 7.800 745 tagctagcatcgatcgatcagtcag 138 25 0 48.00 62.800 18.50 14.32 32.26 7.800 746 ctagcatcgatcgatcagtcagcta 135 25 0 48.00 62.800 31.12 29.68 32.26 7.800 747 tagcatcgatcgatcagtcagctag 134 25 0 48.00 62.800 31.04 18.17 32.26 7.800 748 catcgatcgatcagtcagctagcta 131 25 0 48.00 62.800 17.10 0.00 32.26 7.800 749 actagctagcatcgatcgatcagtc 140 25 0 48.00 62.802 21.52 11.46 32.26 7.802 750 tagtagcatcgatgatcagctagcta 214 26 0 42.31 61.805 21.89 17.81 29.76 7.805 751 atgatcgatcagtcagctagctaaata 281 27 0 37.04 60.831 30.77 3.94 41.24 7.831 752 atgatgatagtagatgatgatcagc 243 25 0 36.00 57.142 0.00 0.00 34.72 7.858 753 gatgatgatagtagatgatgatcagc 244 26 0 38.46 58.126 0.00 0.00 34.72 7.874 754 atgtagcatcgatcagtagctagcta 327 26 0 42.31 62.028 23.52 19.77 35.96 8.028 755 gtagctagcatgatgatcgatcagtc 293 26 0 46.15 62.094 25.60 21.92 45.35 8.094 756 tgatgatagtagatgatgatcagcta 242 26 0 34.62 57.906 11.65 9.10 45.14 8.094 757 atgatgatcgatcagtcagctagcta 284 26 0 42.31 62.255 29.63 14.90 45.35 8.255 758 tgatcgatcagtcagctagctaaatag 280 27 0 40.74 61.304 26.87 0.00 32.65 8.304 759 tagctagcatcgatgatgatcagcta 539 26 0 42.31 62.307 25.79 24.12 42.87 8.307 760 tagcatcgatgatgatcagctagcta 535 26 0 42.31 62.307 22.73 20.77 42.87 8.307 761 tgatgatcgatcagtcagctagctaa 283 26 0 42.31 62.416 28.37 11.24 41.24 8.416 762 tagctagcatgatgatcgatcagtca 292 26 0 42.31 62.472 28.37 27.49 45.35 8.472 763 tagcatgatgatcgatcagtcagcta 288 26 0 42.31 62.472 33.83 32.61 45.35 8.472 764 agtagctagcatgatgatcgatcagt 294 26 0 42.31 62.478 12.39 3.56 45.35 8.478 765 tgtagcatcgatcagtagctagctag 326 26 0 46.15 62.503 24.35 17.46 35.96 8.503 766 tactagctagcatcgatcgatcagtc 141 26 0 46.15 62.543 21.52 11.46 32.26 8.543 767 ctactagctagcatcgatcgatcagt 142 26 0 46.15 62.548 14.05 11.62 32.26 8.548 768 ctactagctagcatcgatgatgatcag 543 27 0 44.44 61.665 16.24 10.83 42.87 8.665 769 catcgatgatgatcagctagctagtag 532 27 0 44.44 61.665 21.68 0.55 42.87 8.665 770 ctagtagctagcatgatgatcgatcag 296 27 0 44.44 61.665 30.77 19.47 45.35 8.665 771 atgatgatagtagatgatgatcagcta 243 27 0 33.33 58.315 11.65 9.10 45.14 8.685 772 gctaaatagctagtagatgatgatga 262 26 0 34.62 57.263 15.95 0.00 46.11 8.737 773 ctagtagcatcgatgatcagctagct 215 26 0 46.15 62.784 26.51 19.92 29.76 8.784 774 agtagcatcgatgatcagctagctag 213 26 0 46.15 62.784 21.48 17.46 29.76 8.784 775 gatgatcgatcagtcagctagctaaat 282 27 0 40.74 61.785 26.17 9.71 41.24 8.785 776 agctagtagatgatgatgatagtaga 255 26 0 34.62 57.155 2.87 0.00 0.00 8.845 777 ctagctagctacgatcgatcagctag 626 26 0 50.00 62.848 40.11 40.11 43.70 8.848 778 tgatgatgatagtagatgatgatcag 245 26 0 34.62 57.026 0.90 0.00 37.84 8.974 779 gatgtagcatcgatcagtagctagct 328 26 0 46.15 62.999 23.74 23.74 35.96 8.999 780 tagtagctagcatgatgatcgatcagt 295 27 0 40.74 62.236 12.39 3.56 45.35 9.236 781 gctaaatagctagtagatgatgatgat 262 27 0 33.33 57.689 15.95 0.00 46.11 9.311 782 agctagtagatgatgatgatagtagat 255 27 0 33.33 57.592 2.87 0.00 0.00 9.408 783 gctagtagatgatgatgatagtagatg 254 27 0 37.04 57.549 2.87 0.00 0.00 9.451 784 gatgatgatgatagtagatgatgatca 247 27 0 33.33 57.467 0.00 0.00 44.47 9.533 785 ctagtagcatcgatgatcagctagcta 215 27 0 44.44 62.534 26.51 20.00 29.76 9.534 786 tagtagcatcgatgatcagctagctag 214 27 0 44.44 62.534 20.52 17.46 29.76 9.534 787 atgatgatgatagtagatgatgatcag 246 27 0 33.33 57.462 0.90 0.00 44.01 9.538 788 atcgatcgatgatagtagctagctagc 452 27 0 44.44 62.627 34.67 34.67 40.92 9.627 789 atgatgatcgatcagtcagctagctaa 284 27 0 40.74 62.665 29.63 14.44 45.35 9.665 790 gatgtagcatcgatcagtagctagcta 328 27 0 44.44 62.742 23.52 19.77 35.96 9.742 791 atgtagcatcgatcagtagctagctag 327 27 0 44.44 62.747 24.35 17.46 35.96 9.747 792 agatgatgatgatagtagatgatgatc 248 27 0 33.33 57.242 0.00 0.00 0.00 9.758 793 tgatgatcgatcagtcagctagctaaa 283 27 0 40.74 62.820 28.37 10.77 41.24 9.820 794 tagctagtagatgatgatgatagtaga 256 27 0 33.33 57.100 2.87 0.00 0.00 9.900 primer3-2.3.6/test/primer1_th_list_last/SP01-02.txt.rev0000664000077300007730000000317712231156243021431 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TGTTGGAGAAAAGCCACTGG 245 20 0 50.00 59.232 0.00 0.00 35.22 0.768 1 TCCTGTTGGAGAAAAGCCAC 248 20 0 50.00 58.942 0.00 0.00 38.11 1.058 2 CCTGTTGGAGAAAAGCCACT 247 20 0 50.00 58.939 0.00 0.00 35.22 1.061 3 CCTGTTGGAGAAAAGCCACTG 247 21 0 52.38 60.308 0.00 0.00 35.22 1.308 4 CTGTTGGAGAAAAGCCACTGG 246 21 0 52.38 60.308 0.00 0.00 35.22 1.308 5 TTCCTGTTGGAGAAAAGCCAC 249 21 0 47.62 59.622 0.00 0.00 44.83 1.378 6 TCCTGTTGGAGAAAAGCCACT 248 21 0 47.62 60.452 0.00 0.00 38.11 1.452 7 TTCCTGTTGGAGAAAAGCCA 249 20 0 45.00 58.190 0.00 0.00 44.83 1.810 8 CTGTTGGAGAAAAGCCACTG 246 20 0 50.00 57.830 0.00 0.00 35.22 2.170 9 TTCCTGTTGGAGAAAAGCCACT 249 22 0 45.45 61.042 0.00 0.00 44.83 3.042 10 TCCTGTTGGAGAAAAGCCA 248 19 0 47.37 57.388 0.00 0.00 38.11 3.612 11 CCTGTTGGAGAAAAGCCAC 247 19 0 52.63 57.326 0.00 0.00 35.22 3.674 12 GTTGGAGAAAAGCCACTGG 244 19 0 52.63 57.326 0.00 0.00 35.22 3.674 13 TCCTGTTGGAGAAAAGCCACTG 248 22 0 50.00 61.688 0.00 0.00 38.11 3.688 14 GACCCCACGCACGCTAAG 159 18 0 66.67 61.698 0.00 0.00 0.00 3.698 15 CCTGTTGGAGAAAAGCCACTGG 247 22 0 54.55 62.587 0.00 0.00 35.22 4.587 16 TTCCTGTTGGAGAAAAGCCACTG 249 23 0 47.83 62.191 0.00 0.00 44.83 5.191 primer3-2.3.6/test/primer1_th_list_last/SP01-19.txt.for0000664000077300007730000002543412231156243021433 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 AACCTGGCTACAGTTCCCTC 72 20 0 55.00 59.951 0.00 0.00 43.93 0.049 1 TATTCCTGTCCCTGTGCCTC 49 20 0 55.00 59.732 0.00 0.00 0.00 0.268 2 AAACCTGGCTACAGTTCCCT 71 20 0 50.00 59.503 0.00 0.00 43.93 0.497 3 CTGTCCCTGTGCCTCAGAAA 54 20 0 55.00 60.532 0.00 0.00 46.81 0.532 4 GCCTCAGAAACCTGGCTACA 64 20 0 55.00 60.603 0.00 0.00 33.11 0.603 5 TGCCTCAGAAACCTGGCTAC 63 20 0 55.00 60.603 1.36 0.00 33.11 0.603 6 GTGCCTCAGAAACCTGGCTA 62 20 0 55.00 60.603 1.36 0.00 33.11 0.603 7 TGTCCCTGTGCCTCAGAAAC 55 20 0 55.00 60.819 6.46 5.40 46.81 0.819 8 GAAACCTGGCTACAGTTCCC 70 20 0 55.00 59.095 0.00 0.00 43.93 0.905 9 ATATTCCTGTCCCTGTGCCT 48 20 0 50.00 59.053 0.00 0.00 0.00 0.947 10 ATTCCTGTCCCTGTGCCTC 50 19 0 57.89 59.938 0.00 0.00 0.00 1.062 11 CTGTCCCTGTGCCTCAGAA 54 19 0 57.89 59.863 0.00 0.00 46.81 1.137 12 CCCTGTGCCTCAGAAACCT 58 19 0 57.89 60.168 7.94 0.00 46.81 1.168 13 TCCCTGTGCCTCAGAAACC 57 19 0 57.89 60.168 7.94 0.00 46.81 1.168 14 ATATTCCTGTCCCTGTGCCTC 48 21 0 52.38 60.172 0.00 0.00 0.00 1.172 15 AATATTCCTGTCCCTGTGCCT 47 21 0 47.62 59.747 0.00 0.00 0.00 1.253 16 CCTCAGAAACCTGGCTACAGT 65 21 0 52.38 60.311 0.00 0.00 43.93 1.311 17 CCTCAGAAACCTGGCTACAG 65 20 0 55.00 58.522 0.00 0.00 44.78 1.478 18 CAGAAACCTGGCTACAGTTCC 68 21 0 52.38 59.491 0.00 0.00 43.93 1.509 19 GTCCCTGTGCCTCAGAAACC 56 20 0 60.00 61.528 4.86 0.00 46.81 1.528 20 CCTGTGCCTCAGAAACCTGG 59 20 0 60.00 61.531 7.94 0.00 46.81 1.531 21 CCCTGTGCCTCAGAAACCTG 58 20 0 60.00 61.531 7.94 0.00 46.81 1.531 22 AAACCTGGCTACAGTTCCCTC 71 21 0 52.38 60.589 0.00 0.00 43.93 1.589 23 GAAACCTGGCTACAGTTCCCT 70 21 0 52.38 60.589 0.00 0.00 43.93 1.589 24 AGAAACCTGGCTACAGTTCCC 69 21 0 52.38 60.589 0.00 0.00 43.93 1.589 25 TGTCCCTGTGCCTCAGAAA 55 19 0 52.63 59.397 0.00 0.00 46.81 1.603 26 TCCCTGTGCCTCAGAAACCT 57 20 0 55.00 61.721 7.94 0.00 46.81 1.721 27 ACCTGGCTACAGTTCCCTC 73 19 0 57.89 59.246 0.00 0.00 43.93 1.754 28 ATTCCTGTCCCTGTGCCTCA 50 20 0 55.00 61.795 0.00 0.00 0.00 1.795 29 AATATTCCTGTCCCTGTGCC 47 20 0 50.00 58.196 0.00 0.00 0.00 1.804 30 TGCCTCAGAAACCTGGCTA 63 19 0 52.63 59.164 1.36 0.00 33.11 1.836 31 GTGCCTCAGAAACCTGGCT 62 19 0 57.89 60.850 1.36 0.00 33.11 1.850 32 AGAAACCTGGCTACAGTTCC 69 20 0 50.00 58.065 0.00 0.00 43.93 1.935 33 GTCCCTGTGCCTCAGAAAC 56 19 0 57.89 58.972 3.11 0.00 46.81 2.028 34 CTGTGCCTCAGAAACCTGG 60 19 0 57.89 58.969 0.98 0.00 46.81 2.031 35 CCTGTGCCTCAGAAACCTG 59 19 0 57.89 58.969 7.94 0.00 46.81 2.031 36 TGTGCCTCAGAAACCTGGC 61 19 0 57.89 61.149 0.00 0.00 33.11 2.149 37 CTGTGCCTCAGAAACCTGGC 60 20 0 60.00 62.156 0.98 0.00 46.81 2.156 38 CTCAGAAACCTGGCTACAGT 66 20 0 50.00 57.783 0.00 0.00 43.93 2.217 39 AACCTGGCTACAGTTCCCT 72 19 0 52.63 58.763 0.00 0.00 43.93 2.237 40 CCTGTCCCTGTGCCTCAG 53 18 0 66.67 60.245 0.00 0.00 43.51 2.245 41 GCCTCAGAAACCTGGCTAC 64 19 0 57.89 58.746 0.00 0.00 33.11 2.254 42 TTCCTGTCCCTGTGCCTCA 51 19 0 57.89 61.407 0.00 0.00 0.00 2.407 43 CTCAGAAACCTGGCTACAGTTC 66 22 0 50.00 59.590 0.00 0.00 43.93 2.410 44 CCTGTCCCTGTGCCTCAGAA 53 20 0 60.00 62.423 0.00 0.00 46.81 2.423 45 TTCCTGTCCCTGTGCCTCAG 51 20 0 60.00 62.423 0.00 0.00 43.51 2.423 46 TAATATTCCTGTCCCTGTGCCT 46 22 0 45.45 59.565 0.00 0.00 0.00 2.435 47 TATTCCTGTCCCTGTGCCT 49 19 0 52.63 58.528 0.00 0.00 0.00 2.472 48 TCAGAAACCTGGCTACAGTTC 67 21 0 47.62 58.522 0.00 0.00 43.93 2.478 49 CTCAGAAACCTGGCTACAGTT 66 21 0 47.62 58.518 0.00 0.00 43.93 2.482 50 TATTCCTGTCCCTGTGCCTCA 49 21 0 52.38 61.511 0.00 0.00 0.00 2.511 51 TTCCTGTCCCTGTGCCTC 51 18 0 61.11 59.440 0.00 0.00 0.00 2.560 52 GCCTCAGAAACCTGGCTACAG 64 21 0 57.14 61.600 0.00 0.00 44.78 2.600 53 TGCCTCAGAAACCTGGCT 63 18 0 55.56 59.352 1.36 0.00 33.11 2.648 54 TGTGCCTCAGAAACCTGGCT 61 20 0 55.00 62.654 1.36 0.00 33.11 2.654 55 TCAGAAACCTGGCTACAGTT 67 20 0 45.00 57.313 0.00 0.00 43.93 2.687 56 TCCTGTCCCTGTGCCTCA 52 18 0 61.11 60.738 0.00 0.00 0.00 2.738 57 AATATTCCTGTCCCTGTGCCTC 47 22 0 50.00 60.773 0.00 0.00 0.00 2.773 58 GTGCCTCAGAAACCTGGC 62 18 0 61.11 59.225 0.00 0.00 33.11 2.775 59 CTGTCCCTGTGCCTCAGAAAC 54 21 0 57.14 61.802 6.46 5.40 46.81 2.802 60 CCTGTCCCTGTGCCTCAGA 53 19 0 63.16 61.850 7.94 0.00 46.81 2.850 61 TCCTGTCCCTGTGCCTCAG 52 19 0 63.16 61.850 0.00 0.00 43.51 2.850 62 GTGCCTCAGAAACCTGGCTAC 62 21 0 57.14 61.867 0.00 0.00 33.11 2.867 63 CTGTCCCTGTGCCTCAGA 54 18 0 61.11 59.122 7.94 0.00 46.81 2.878 64 TCAGAAACCTGGCTACAGTTCC 67 22 0 50.00 60.900 0.00 0.00 43.93 2.900 65 CCTCAGAAACCTGGCTACAGTT 65 22 0 50.00 60.901 0.00 0.00 43.93 2.901 66 TAATATTCCTGTCCCTGTGCC 46 21 0 47.62 58.080 0.00 0.00 0.00 2.920 67 AAACCTGGCTACAGTTCCC 71 19 0 52.63 57.870 0.00 0.00 43.93 3.130 68 TTAATATTCCTGTCCCTGTGCCT 45 23 0 43.48 60.175 0.00 0.00 0.00 3.175 69 TTAATATTCCTGTCCCTGTGCC 45 22 0 45.45 58.776 0.00 0.00 0.00 3.224 70 ATTCCTGTCCCTGTGCCT 50 18 0 55.56 58.683 0.00 0.00 0.00 3.317 71 TGCCTCAGAAACCTGGCTACA 63 21 0 52.38 62.333 1.36 0.00 33.11 3.333 72 TGTGCCTCAGAAACCTGGCTA 61 21 0 52.38 62.333 1.36 0.00 33.11 3.333 73 TGTCCCTGTGCCTCAGAA 55 18 0 55.56 58.619 0.00 0.00 46.81 3.381 74 CCCTGTGCCTCAGAAACC 58 18 0 61.11 58.501 7.94 0.00 46.81 3.499 75 GAAACCTGGCTACAGTTCCCTC 70 22 0 54.55 61.548 0.00 0.00 43.93 3.548 76 TAATATTCCTGTCCCTGTGCCTC 46 23 0 47.83 60.559 0.00 0.00 0.00 3.559 77 ATATTCCTGTCCCTGTGCC 48 19 0 52.63 57.398 0.00 0.00 0.00 3.602 78 CCTCAGAAACCTGGCTACA 65 19 0 52.63 57.267 0.00 0.00 33.11 3.733 79 ATTCCTGTCCCTGTGCCTCAG 50 21 0 57.14 62.743 0.00 0.00 43.51 3.743 80 ATTAATATTCCTGTCCCTGTGCC 44 23 0 43.48 59.217 0.00 0.00 0.00 3.783 81 CAGAAACCTGGCTACAGTTCCC 68 22 0 54.55 61.808 0.00 0.00 43.93 3.808 82 ATATTCCTGTCCCTGTGCCTCA 48 22 0 50.00 61.861 0.00 0.00 0.00 3.861 83 TCCCTGTGCCTCAGAAACCTG 57 21 0 57.14 62.942 0.00 0.00 46.81 3.942 84 CCTGTCCCTGTGCCTCAGAAA 53 21 0 57.14 62.942 0.00 0.00 46.81 3.942 85 GTCCCTGTGCCTCAGAAACCT 56 21 0 57.14 62.943 4.86 0.00 46.81 3.943 86 AGAAACCTGGCTACAGTTCCCT 69 22 0 50.00 61.994 0.00 0.00 43.93 3.994 87 ACCTGGCTACAGTTCCCT 73 18 0 55.56 57.939 0.00 0.00 43.93 4.061 88 TGTGCCTCAGAAACCTGG 61 18 0 55.56 57.684 0.00 0.00 33.11 4.316 89 TATTCCTGTCCCTGTGCCTCAG 49 22 0 54.55 62.434 0.00 0.00 43.51 4.434 90 ATTAATATTCCTGTCCCTGTGCCT 44 24 0 41.67 60.550 0.00 0.00 0.00 4.550 91 TCCCTGTGCCTCAGAAAC 57 18 0 55.56 57.361 7.94 0.00 46.81 4.639 92 GTCCCTGTGCCTCAGAAA 56 18 0 55.56 57.361 0.00 0.00 46.81 4.639 93 CCTGTGCCTCAGAAACCT 59 18 0 55.56 57.353 7.94 0.00 46.81 4.647 94 CCTGGCTACAGTTCCCTC 74 18 0 61.11 57.215 0.00 0.00 43.93 4.785 95 CTCAGAAACCTGGCTACAGTTCC 66 23 0 52.17 61.799 0.00 0.00 43.93 4.799 96 CCTCAGAAACCTGGCTACAGTTC 65 23 0 52.17 61.799 0.00 0.00 43.93 4.799 97 GCCTCAGAAACCTGGCTA 64 18 0 55.56 57.116 0.00 0.00 33.11 4.884 98 TATTAATATTCCTGTCCCTGTGCC 43 24 0 41.67 59.073 0.00 0.00 0.00 4.927 99 AACCTGGCTACAGTTCCC 72 18 0 55.56 57.014 0.00 0.00 43.93 4.986 100 TTAATATTCCTGTCCCTGTGCCTC 45 24 0 45.83 61.094 0.00 0.00 0.00 5.094 101 TATTAATATTCCTGTCCCTGTGCCT 43 25 0 40.00 60.358 0.00 0.00 0.00 5.358 102 AATATTCCTGTCCCTGTGCCTCA 47 23 0 47.83 62.368 0.00 0.00 0.00 5.368 103 ATATTAATATTCCTGTCCCTGTGCC 42 25 0 40.00 59.470 2.66 0.00 0.00 5.530 104 ATATTCCTGTCCCTGTGCCTCAG 48 23 0 52.17 62.727 0.00 0.00 43.51 5.727 105 AGAAACCTGGCTACAGTTCCCTC 69 23 0 52.17 62.847 0.00 0.00 43.93 5.847 106 AATATTAATATTCCTGTCCCTGTGCC 41 26 0 38.46 60.010 14.65 0.00 0.00 6.010 107 TAATATTCCTGTCCCTGTGCCTCA 46 24 0 45.83 62.095 0.00 0.00 0.00 6.095 108 ATTAATATTCCTGTCCCTGTGCCTC 44 25 0 44.00 61.415 0.00 0.00 0.00 6.415 109 ATATTAATATTCCTGTCCCTGTGCCT 42 26 0 38.46 60.698 2.66 0.00 0.00 6.698 110 ATATTAATATTCCTGTCCCTGTGC 42 24 0 37.50 57.298 2.66 0.00 0.00 6.702 111 AATATTAATATTCCTGTCCCTGTGC 41 25 0 36.00 57.944 14.65 0.00 0.00 7.056 112 TATTAATATTCCTGTCCCTGTGCCTC 43 26 0 42.31 61.200 0.00 0.00 0.00 7.200 113 CAAATATTAATATTCCTGTCCCTGTGC 39 27 0 37.04 59.644 14.75 0.00 32.85 7.356 114 AAATATTAATATTCCTGTCCCTGTGC 40 26 0 34.62 58.541 19.49 0.00 32.85 7.459 115 AAATATTAATATTCCTGTCCCTGTGCC 40 27 0 37.04 60.509 19.49 0.00 32.85 7.509 116 TTAATATTCCTGTCCCTGTGCCTCA 45 25 0 44.00 62.553 0.00 0.00 0.00 7.553 117 TAATATTCCTGTCCCTGTGCCTCAG 46 25 0 48.00 62.882 0.00 0.00 43.51 7.882 118 AATATTAATATTCCTGTCCCTGTGCCT 41 27 0 37.04 61.178 14.65 0.00 0.00 8.178 119 ATATTAATATTCCTGTCCCTGTGCCTC 42 27 0 40.74 61.495 2.66 0.00 0.00 8.495 120 TCAAATATTAATATTCCTGTCCCTGTG 38 27 0 33.33 58.380 17.39 10.47 32.85 8.620 121 TCAAATATTAATATTCCTGTCCCTGT 38 26 0 30.77 57.195 17.39 0.96 32.85 8.805 122 ATTAATATTCCTGTCCCTGTGCCTCA 44 26 0 42.31 62.812 0.00 0.00 0.00 8.812 123 CTCAAATATTAATATTCCTGTCCCTGT 37 27 0 33.33 58.156 7.13 0.00 32.85 8.844 124 CAAATATTAATATTCCTGTCCCTGTG 39 26 0 34.62 57.154 14.75 5.12 32.85 8.846 125 TATTAATATTCCTGTCCCTGTGCCTCA 43 27 0 40.74 62.553 0.00 0.00 0.00 9.553 primer3-2.3.6/test/primer1_th_list_last/AGAG.rev0000664000077300007730000007065112231156243020411 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TCTCTCTCTCTCTCTCTCTCTCT 155 23 0 47.83 58.820 0.00 0.00 0.00 4.180 1 TCTCTCTCTCTCTCTCTCTCTCT 153 23 0 47.83 58.820 0.00 0.00 0.00 4.180 2 TCTCTCTCTCTCTCTCTCTCTCT 151 23 0 47.83 58.820 0.00 0.00 0.00 4.180 3 TCTCTCTCTCTCTCTCTCTCTCT 149 23 0 47.83 58.820 0.00 0.00 0.00 4.180 4 TCTCTCTCTCTCTCTCTCTCTCT 147 23 0 47.83 58.820 0.00 0.00 0.00 4.180 5 TCTCTCTCTCTCTCTCTCTCTCT 145 23 0 47.83 58.820 0.00 0.00 0.00 4.180 6 TCTCTCTCTCTCTCTCTCTCTCT 143 23 0 47.83 58.820 0.00 0.00 0.00 4.180 7 TCTCTCTCTCTCTCTCTCTCTCT 141 23 0 47.83 58.820 0.00 0.00 0.00 4.180 8 TCTCTCTCTCTCTCTCTCTCTCT 139 23 0 47.83 58.820 0.00 0.00 0.00 4.180 9 TCTCTCTCTCTCTCTCTCTCTCT 137 23 0 47.83 58.820 0.00 0.00 0.00 4.180 10 TCTCTCTCTCTCTCTCTCTCTCT 135 23 0 47.83 58.820 0.00 0.00 0.00 4.180 11 TCTCTCTCTCTCTCTCTCTCTCT 133 23 0 47.83 58.820 0.00 0.00 0.00 4.180 12 TCTCTCTCTCTCTCTCTCTCTCT 131 23 0 47.83 58.820 0.00 0.00 0.00 4.180 13 TCTCTCTCTCTCTCTCTCTCTCT 129 23 0 47.83 58.820 0.00 0.00 0.00 4.180 14 TCTCTCTCTCTCTCTCTCTCTCT 127 23 0 47.83 58.820 0.00 0.00 0.00 4.180 15 TCTCTCTCTCTCTCTCTCTCTCT 125 23 0 47.83 58.820 0.00 0.00 0.00 4.180 16 TCTCTCTCTCTCTCTCTCTCTCT 123 23 0 47.83 58.820 0.00 0.00 0.00 4.180 17 TCTCTCTCTCTCTCTCTCTCTCT 121 23 0 47.83 58.820 0.00 0.00 0.00 4.180 18 TCTCTCTCTCTCTCTCTCTCTCT 119 23 0 47.83 58.820 0.00 0.00 0.00 4.180 19 TCTCTCTCTCTCTCTCTCTCTCT 117 23 0 47.83 58.820 0.00 0.00 0.00 4.180 20 TCTCTCTCTCTCTCTCTCTCTCT 115 23 0 47.83 58.820 0.00 0.00 0.00 4.180 21 TCTCTCTCTCTCTCTCTCTCTCT 113 23 0 47.83 58.820 0.00 0.00 0.00 4.180 22 TCTCTCTCTCTCTCTCTCTCTCT 111 23 0 47.83 58.820 0.00 0.00 0.00 4.180 23 TCTCTCTCTCTCTCTCTCTCTCT 109 23 0 47.83 58.820 0.00 0.00 0.00 4.180 24 TCTCTCTCTCTCTCTCTCTCTCT 107 23 0 47.83 58.820 0.00 0.00 0.00 4.180 25 TCTCTCTCTCTCTCTCTCTCTCT 105 23 0 47.83 58.820 0.00 0.00 0.00 4.180 26 TCTCTCTCTCTCTCTCTCTCTCT 103 23 0 47.83 58.820 0.00 0.00 0.00 4.180 27 TCTCTCTCTCTCTCTCTCTCTCT 101 23 0 47.83 58.820 0.00 0.00 0.00 4.180 28 TCTCTCTCTCTCTCTCTCTCTCTC 155 24 0 50.00 59.811 0.00 0.00 0.00 4.189 29 TCTCTCTCTCTCTCTCTCTCTCTC 153 24 0 50.00 59.811 0.00 0.00 0.00 4.189 30 TCTCTCTCTCTCTCTCTCTCTCTC 151 24 0 50.00 59.811 0.00 0.00 0.00 4.189 31 TCTCTCTCTCTCTCTCTCTCTCTC 149 24 0 50.00 59.811 0.00 0.00 0.00 4.189 32 TCTCTCTCTCTCTCTCTCTCTCTC 147 24 0 50.00 59.811 0.00 0.00 0.00 4.189 33 TCTCTCTCTCTCTCTCTCTCTCTC 145 24 0 50.00 59.811 0.00 0.00 0.00 4.189 34 TCTCTCTCTCTCTCTCTCTCTCTC 143 24 0 50.00 59.811 0.00 0.00 0.00 4.189 35 TCTCTCTCTCTCTCTCTCTCTCTC 141 24 0 50.00 59.811 0.00 0.00 0.00 4.189 36 TCTCTCTCTCTCTCTCTCTCTCTC 139 24 0 50.00 59.811 0.00 0.00 0.00 4.189 37 TCTCTCTCTCTCTCTCTCTCTCTC 137 24 0 50.00 59.811 0.00 0.00 0.00 4.189 38 TCTCTCTCTCTCTCTCTCTCTCTC 135 24 0 50.00 59.811 0.00 0.00 0.00 4.189 39 TCTCTCTCTCTCTCTCTCTCTCTC 133 24 0 50.00 59.811 0.00 0.00 0.00 4.189 40 TCTCTCTCTCTCTCTCTCTCTCTC 131 24 0 50.00 59.811 0.00 0.00 0.00 4.189 41 TCTCTCTCTCTCTCTCTCTCTCTC 129 24 0 50.00 59.811 0.00 0.00 0.00 4.189 42 TCTCTCTCTCTCTCTCTCTCTCTC 127 24 0 50.00 59.811 0.00 0.00 0.00 4.189 43 TCTCTCTCTCTCTCTCTCTCTCTC 125 24 0 50.00 59.811 0.00 0.00 0.00 4.189 44 TCTCTCTCTCTCTCTCTCTCTCTC 123 24 0 50.00 59.811 0.00 0.00 0.00 4.189 45 TCTCTCTCTCTCTCTCTCTCTCTC 121 24 0 50.00 59.811 0.00 0.00 0.00 4.189 46 TCTCTCTCTCTCTCTCTCTCTCTC 119 24 0 50.00 59.811 0.00 0.00 0.00 4.189 47 TCTCTCTCTCTCTCTCTCTCTCTC 117 24 0 50.00 59.811 0.00 0.00 0.00 4.189 48 TCTCTCTCTCTCTCTCTCTCTCTC 115 24 0 50.00 59.811 0.00 0.00 0.00 4.189 49 TCTCTCTCTCTCTCTCTCTCTCTC 113 24 0 50.00 59.811 0.00 0.00 0.00 4.189 50 TCTCTCTCTCTCTCTCTCTCTCTC 111 24 0 50.00 59.811 0.00 0.00 0.00 4.189 51 TCTCTCTCTCTCTCTCTCTCTCTC 109 24 0 50.00 59.811 0.00 0.00 0.00 4.189 52 TCTCTCTCTCTCTCTCTCTCTCTC 107 24 0 50.00 59.811 0.00 0.00 0.00 4.189 53 TCTCTCTCTCTCTCTCTCTCTCTC 105 24 0 50.00 59.811 0.00 0.00 0.00 4.189 54 TCTCTCTCTCTCTCTCTCTCTCTC 103 24 0 50.00 59.811 0.00 0.00 0.00 4.189 55 TCTCTCTCTCTCTCTCTCTCTCTC 101 24 0 50.00 59.811 0.00 0.00 0.00 4.189 56 CTCTCTCTCTCTCTCTCTCTCTCT 154 24 0 50.00 59.810 0.00 0.00 0.00 4.190 57 CTCTCTCTCTCTCTCTCTCTCTCT 152 24 0 50.00 59.810 0.00 0.00 0.00 4.190 58 CTCTCTCTCTCTCTCTCTCTCTCT 150 24 0 50.00 59.810 0.00 0.00 0.00 4.190 59 CTCTCTCTCTCTCTCTCTCTCTCT 148 24 0 50.00 59.810 0.00 0.00 0.00 4.190 60 CTCTCTCTCTCTCTCTCTCTCTCT 146 24 0 50.00 59.810 0.00 0.00 0.00 4.190 61 CTCTCTCTCTCTCTCTCTCTCTCT 144 24 0 50.00 59.810 0.00 0.00 0.00 4.190 62 CTCTCTCTCTCTCTCTCTCTCTCT 142 24 0 50.00 59.810 0.00 0.00 0.00 4.190 63 CTCTCTCTCTCTCTCTCTCTCTCT 140 24 0 50.00 59.810 0.00 0.00 0.00 4.190 64 CTCTCTCTCTCTCTCTCTCTCTCT 138 24 0 50.00 59.810 0.00 0.00 0.00 4.190 65 CTCTCTCTCTCTCTCTCTCTCTCT 136 24 0 50.00 59.810 0.00 0.00 0.00 4.190 66 CTCTCTCTCTCTCTCTCTCTCTCT 134 24 0 50.00 59.810 0.00 0.00 0.00 4.190 67 CTCTCTCTCTCTCTCTCTCTCTCT 132 24 0 50.00 59.810 0.00 0.00 0.00 4.190 68 CTCTCTCTCTCTCTCTCTCTCTCT 130 24 0 50.00 59.810 0.00 0.00 0.00 4.190 69 CTCTCTCTCTCTCTCTCTCTCTCT 128 24 0 50.00 59.810 0.00 0.00 0.00 4.190 70 CTCTCTCTCTCTCTCTCTCTCTCT 126 24 0 50.00 59.810 0.00 0.00 0.00 4.190 71 CTCTCTCTCTCTCTCTCTCTCTCT 124 24 0 50.00 59.810 0.00 0.00 0.00 4.190 72 CTCTCTCTCTCTCTCTCTCTCTCT 122 24 0 50.00 59.810 0.00 0.00 0.00 4.190 73 CTCTCTCTCTCTCTCTCTCTCTCT 120 24 0 50.00 59.810 0.00 0.00 0.00 4.190 74 CTCTCTCTCTCTCTCTCTCTCTCT 118 24 0 50.00 59.810 0.00 0.00 0.00 4.190 75 CTCTCTCTCTCTCTCTCTCTCTCT 116 24 0 50.00 59.810 0.00 0.00 0.00 4.190 76 CTCTCTCTCTCTCTCTCTCTCTCT 114 24 0 50.00 59.810 0.00 0.00 0.00 4.190 77 CTCTCTCTCTCTCTCTCTCTCTCT 112 24 0 50.00 59.810 0.00 0.00 0.00 4.190 78 CTCTCTCTCTCTCTCTCTCTCTCT 110 24 0 50.00 59.810 0.00 0.00 0.00 4.190 79 CTCTCTCTCTCTCTCTCTCTCTCT 108 24 0 50.00 59.810 0.00 0.00 0.00 4.190 80 CTCTCTCTCTCTCTCTCTCTCTCT 106 24 0 50.00 59.810 0.00 0.00 0.00 4.190 81 CTCTCTCTCTCTCTCTCTCTCTCT 104 24 0 50.00 59.810 0.00 0.00 0.00 4.190 82 CTCTCTCTCTCTCTCTCTCTCTCT 102 24 0 50.00 59.810 0.00 0.00 0.00 4.190 83 CTCTCTCTCTCTCTCTCTCTCTCT 100 24 0 50.00 59.810 0.00 0.00 0.00 4.190 84 CTCTCTCTCTCTCTCTCTCTCTC 154 23 0 52.17 58.478 0.00 0.00 0.00 4.522 85 CTCTCTCTCTCTCTCTCTCTCTC 152 23 0 52.17 58.478 0.00 0.00 0.00 4.522 86 CTCTCTCTCTCTCTCTCTCTCTC 150 23 0 52.17 58.478 0.00 0.00 0.00 4.522 87 CTCTCTCTCTCTCTCTCTCTCTC 148 23 0 52.17 58.478 0.00 0.00 0.00 4.522 88 CTCTCTCTCTCTCTCTCTCTCTC 146 23 0 52.17 58.478 0.00 0.00 0.00 4.522 89 CTCTCTCTCTCTCTCTCTCTCTC 144 23 0 52.17 58.478 0.00 0.00 0.00 4.522 90 CTCTCTCTCTCTCTCTCTCTCTC 142 23 0 52.17 58.478 0.00 0.00 0.00 4.522 91 CTCTCTCTCTCTCTCTCTCTCTC 140 23 0 52.17 58.478 0.00 0.00 0.00 4.522 92 CTCTCTCTCTCTCTCTCTCTCTC 138 23 0 52.17 58.478 0.00 0.00 0.00 4.522 93 CTCTCTCTCTCTCTCTCTCTCTC 136 23 0 52.17 58.478 0.00 0.00 0.00 4.522 94 CTCTCTCTCTCTCTCTCTCTCTC 134 23 0 52.17 58.478 0.00 0.00 0.00 4.522 95 CTCTCTCTCTCTCTCTCTCTCTC 132 23 0 52.17 58.478 0.00 0.00 0.00 4.522 96 CTCTCTCTCTCTCTCTCTCTCTC 130 23 0 52.17 58.478 0.00 0.00 0.00 4.522 97 CTCTCTCTCTCTCTCTCTCTCTC 128 23 0 52.17 58.478 0.00 0.00 0.00 4.522 98 CTCTCTCTCTCTCTCTCTCTCTC 126 23 0 52.17 58.478 0.00 0.00 0.00 4.522 99 CTCTCTCTCTCTCTCTCTCTCTC 124 23 0 52.17 58.478 0.00 0.00 0.00 4.522 100 CTCTCTCTCTCTCTCTCTCTCTC 122 23 0 52.17 58.478 0.00 0.00 0.00 4.522 101 CTCTCTCTCTCTCTCTCTCTCTC 120 23 0 52.17 58.478 0.00 0.00 0.00 4.522 102 CTCTCTCTCTCTCTCTCTCTCTC 118 23 0 52.17 58.478 0.00 0.00 0.00 4.522 103 CTCTCTCTCTCTCTCTCTCTCTC 116 23 0 52.17 58.478 0.00 0.00 0.00 4.522 104 CTCTCTCTCTCTCTCTCTCTCTC 114 23 0 52.17 58.478 0.00 0.00 0.00 4.522 105 CTCTCTCTCTCTCTCTCTCTCTC 112 23 0 52.17 58.478 0.00 0.00 0.00 4.522 106 CTCTCTCTCTCTCTCTCTCTCTC 110 23 0 52.17 58.478 0.00 0.00 0.00 4.522 107 CTCTCTCTCTCTCTCTCTCTCTC 108 23 0 52.17 58.478 0.00 0.00 0.00 4.522 108 CTCTCTCTCTCTCTCTCTCTCTC 106 23 0 52.17 58.478 0.00 0.00 0.00 4.522 109 CTCTCTCTCTCTCTCTCTCTCTC 104 23 0 52.17 58.478 0.00 0.00 0.00 4.522 110 CTCTCTCTCTCTCTCTCTCTCTC 102 23 0 52.17 58.478 0.00 0.00 0.00 4.522 111 CTCTCTCTCTCTCTCTCTCTCTC 100 23 0 52.17 58.478 0.00 0.00 0.00 4.522 112 TCTCTCTCTCTCTCTCTCTCTC 155 22 0 50.00 57.383 0.00 0.00 0.00 4.617 113 TCTCTCTCTCTCTCTCTCTCTC 153 22 0 50.00 57.383 0.00 0.00 0.00 4.617 114 TCTCTCTCTCTCTCTCTCTCTC 151 22 0 50.00 57.383 0.00 0.00 0.00 4.617 115 TCTCTCTCTCTCTCTCTCTCTC 149 22 0 50.00 57.383 0.00 0.00 0.00 4.617 116 TCTCTCTCTCTCTCTCTCTCTC 147 22 0 50.00 57.383 0.00 0.00 0.00 4.617 117 TCTCTCTCTCTCTCTCTCTCTC 145 22 0 50.00 57.383 0.00 0.00 0.00 4.617 118 TCTCTCTCTCTCTCTCTCTCTC 143 22 0 50.00 57.383 0.00 0.00 0.00 4.617 119 TCTCTCTCTCTCTCTCTCTCTC 141 22 0 50.00 57.383 0.00 0.00 0.00 4.617 120 TCTCTCTCTCTCTCTCTCTCTC 139 22 0 50.00 57.383 0.00 0.00 0.00 4.617 121 TCTCTCTCTCTCTCTCTCTCTC 137 22 0 50.00 57.383 0.00 0.00 0.00 4.617 122 TCTCTCTCTCTCTCTCTCTCTC 135 22 0 50.00 57.383 0.00 0.00 0.00 4.617 123 TCTCTCTCTCTCTCTCTCTCTC 133 22 0 50.00 57.383 0.00 0.00 0.00 4.617 124 TCTCTCTCTCTCTCTCTCTCTC 131 22 0 50.00 57.383 0.00 0.00 0.00 4.617 125 TCTCTCTCTCTCTCTCTCTCTC 129 22 0 50.00 57.383 0.00 0.00 0.00 4.617 126 TCTCTCTCTCTCTCTCTCTCTC 127 22 0 50.00 57.383 0.00 0.00 0.00 4.617 127 TCTCTCTCTCTCTCTCTCTCTC 125 22 0 50.00 57.383 0.00 0.00 0.00 4.617 128 TCTCTCTCTCTCTCTCTCTCTC 123 22 0 50.00 57.383 0.00 0.00 0.00 4.617 129 TCTCTCTCTCTCTCTCTCTCTC 121 22 0 50.00 57.383 0.00 0.00 0.00 4.617 130 TCTCTCTCTCTCTCTCTCTCTC 119 22 0 50.00 57.383 0.00 0.00 0.00 4.617 131 TCTCTCTCTCTCTCTCTCTCTC 117 22 0 50.00 57.383 0.00 0.00 0.00 4.617 132 TCTCTCTCTCTCTCTCTCTCTC 115 22 0 50.00 57.383 0.00 0.00 0.00 4.617 133 TCTCTCTCTCTCTCTCTCTCTC 113 22 0 50.00 57.383 0.00 0.00 0.00 4.617 134 TCTCTCTCTCTCTCTCTCTCTC 111 22 0 50.00 57.383 0.00 0.00 0.00 4.617 135 TCTCTCTCTCTCTCTCTCTCTC 109 22 0 50.00 57.383 0.00 0.00 0.00 4.617 136 TCTCTCTCTCTCTCTCTCTCTC 107 22 0 50.00 57.383 0.00 0.00 0.00 4.617 137 TCTCTCTCTCTCTCTCTCTCTC 105 22 0 50.00 57.383 0.00 0.00 0.00 4.617 138 TCTCTCTCTCTCTCTCTCTCTC 103 22 0 50.00 57.383 0.00 0.00 0.00 4.617 139 TCTCTCTCTCTCTCTCTCTCTC 101 22 0 50.00 57.383 0.00 0.00 0.00 4.617 140 CTCTCTCTCTCTCTCTCTCTCT 154 22 0 50.00 57.376 0.00 0.00 0.00 4.624 141 CTCTCTCTCTCTCTCTCTCTCT 152 22 0 50.00 57.376 0.00 0.00 0.00 4.624 142 CTCTCTCTCTCTCTCTCTCTCT 150 22 0 50.00 57.376 0.00 0.00 0.00 4.624 143 CTCTCTCTCTCTCTCTCTCTCT 148 22 0 50.00 57.376 0.00 0.00 0.00 4.624 144 CTCTCTCTCTCTCTCTCTCTCT 146 22 0 50.00 57.376 0.00 0.00 0.00 4.624 145 CTCTCTCTCTCTCTCTCTCTCT 144 22 0 50.00 57.376 0.00 0.00 0.00 4.624 146 CTCTCTCTCTCTCTCTCTCTCT 142 22 0 50.00 57.376 0.00 0.00 0.00 4.624 147 CTCTCTCTCTCTCTCTCTCTCT 140 22 0 50.00 57.376 0.00 0.00 0.00 4.624 148 CTCTCTCTCTCTCTCTCTCTCT 138 22 0 50.00 57.376 0.00 0.00 0.00 4.624 149 CTCTCTCTCTCTCTCTCTCTCT 136 22 0 50.00 57.376 0.00 0.00 0.00 4.624 150 CTCTCTCTCTCTCTCTCTCTCT 134 22 0 50.00 57.376 0.00 0.00 0.00 4.624 151 CTCTCTCTCTCTCTCTCTCTCT 132 22 0 50.00 57.376 0.00 0.00 0.00 4.624 152 CTCTCTCTCTCTCTCTCTCTCT 130 22 0 50.00 57.376 0.00 0.00 0.00 4.624 153 CTCTCTCTCTCTCTCTCTCTCT 128 22 0 50.00 57.376 0.00 0.00 0.00 4.624 154 CTCTCTCTCTCTCTCTCTCTCT 126 22 0 50.00 57.376 0.00 0.00 0.00 4.624 155 CTCTCTCTCTCTCTCTCTCTCT 124 22 0 50.00 57.376 0.00 0.00 0.00 4.624 156 CTCTCTCTCTCTCTCTCTCTCT 122 22 0 50.00 57.376 0.00 0.00 0.00 4.624 157 CTCTCTCTCTCTCTCTCTCTCT 120 22 0 50.00 57.376 0.00 0.00 0.00 4.624 158 CTCTCTCTCTCTCTCTCTCTCT 118 22 0 50.00 57.376 0.00 0.00 0.00 4.624 159 CTCTCTCTCTCTCTCTCTCTCT 116 22 0 50.00 57.376 0.00 0.00 0.00 4.624 160 CTCTCTCTCTCTCTCTCTCTCT 114 22 0 50.00 57.376 0.00 0.00 0.00 4.624 161 CTCTCTCTCTCTCTCTCTCTCT 112 22 0 50.00 57.376 0.00 0.00 0.00 4.624 162 CTCTCTCTCTCTCTCTCTCTCT 110 22 0 50.00 57.376 0.00 0.00 0.00 4.624 163 CTCTCTCTCTCTCTCTCTCTCT 108 22 0 50.00 57.376 0.00 0.00 0.00 4.624 164 CTCTCTCTCTCTCTCTCTCTCT 106 22 0 50.00 57.376 0.00 0.00 0.00 4.624 165 CTCTCTCTCTCTCTCTCTCTCT 104 22 0 50.00 57.376 0.00 0.00 0.00 4.624 166 CTCTCTCTCTCTCTCTCTCTCT 102 22 0 50.00 57.376 0.00 0.00 0.00 4.624 167 CTCTCTCTCTCTCTCTCTCTCT 100 22 0 50.00 57.376 0.00 0.00 0.00 4.624 168 CTCTCTCTCTCTCTCTCTCTCTCTC 154 25 0 52.00 60.701 0.00 0.00 0.00 5.701 169 CTCTCTCTCTCTCTCTCTCTCTCTC 152 25 0 52.00 60.701 0.00 0.00 0.00 5.701 170 CTCTCTCTCTCTCTCTCTCTCTCTC 150 25 0 52.00 60.701 0.00 0.00 0.00 5.701 171 CTCTCTCTCTCTCTCTCTCTCTCTC 148 25 0 52.00 60.701 0.00 0.00 0.00 5.701 172 CTCTCTCTCTCTCTCTCTCTCTCTC 146 25 0 52.00 60.701 0.00 0.00 0.00 5.701 173 CTCTCTCTCTCTCTCTCTCTCTCTC 144 25 0 52.00 60.701 0.00 0.00 0.00 5.701 174 CTCTCTCTCTCTCTCTCTCTCTCTC 142 25 0 52.00 60.701 0.00 0.00 0.00 5.701 175 CTCTCTCTCTCTCTCTCTCTCTCTC 140 25 0 52.00 60.701 0.00 0.00 0.00 5.701 176 CTCTCTCTCTCTCTCTCTCTCTCTC 138 25 0 52.00 60.701 0.00 0.00 0.00 5.701 177 CTCTCTCTCTCTCTCTCTCTCTCTC 136 25 0 52.00 60.701 0.00 0.00 0.00 5.701 178 CTCTCTCTCTCTCTCTCTCTCTCTC 134 25 0 52.00 60.701 0.00 0.00 0.00 5.701 179 CTCTCTCTCTCTCTCTCTCTCTCTC 132 25 0 52.00 60.701 0.00 0.00 0.00 5.701 180 CTCTCTCTCTCTCTCTCTCTCTCTC 130 25 0 52.00 60.701 0.00 0.00 0.00 5.701 181 CTCTCTCTCTCTCTCTCTCTCTCTC 128 25 0 52.00 60.701 0.00 0.00 0.00 5.701 182 CTCTCTCTCTCTCTCTCTCTCTCTC 126 25 0 52.00 60.701 0.00 0.00 0.00 5.701 183 CTCTCTCTCTCTCTCTCTCTCTCTC 124 25 0 52.00 60.701 0.00 0.00 0.00 5.701 184 CTCTCTCTCTCTCTCTCTCTCTCTC 122 25 0 52.00 60.701 0.00 0.00 0.00 5.701 185 CTCTCTCTCTCTCTCTCTCTCTCTC 120 25 0 52.00 60.701 0.00 0.00 0.00 5.701 186 CTCTCTCTCTCTCTCTCTCTCTCTC 118 25 0 52.00 60.701 0.00 0.00 0.00 5.701 187 CTCTCTCTCTCTCTCTCTCTCTCTC 116 25 0 52.00 60.701 0.00 0.00 0.00 5.701 188 CTCTCTCTCTCTCTCTCTCTCTCTC 114 25 0 52.00 60.701 0.00 0.00 0.00 5.701 189 CTCTCTCTCTCTCTCTCTCTCTCTC 112 25 0 52.00 60.701 0.00 0.00 0.00 5.701 190 CTCTCTCTCTCTCTCTCTCTCTCTC 110 25 0 52.00 60.701 0.00 0.00 0.00 5.701 191 CTCTCTCTCTCTCTCTCTCTCTCTC 108 25 0 52.00 60.701 0.00 0.00 0.00 5.701 192 CTCTCTCTCTCTCTCTCTCTCTCTC 106 25 0 52.00 60.701 0.00 0.00 0.00 5.701 193 CTCTCTCTCTCTCTCTCTCTCTCTC 104 25 0 52.00 60.701 0.00 0.00 0.00 5.701 194 CTCTCTCTCTCTCTCTCTCTCTCTC 102 25 0 52.00 60.701 0.00 0.00 0.00 5.701 195 CTCTCTCTCTCTCTCTCTCTCTCTC 100 25 0 52.00 60.701 0.00 0.00 0.00 5.701 196 TCTCTCTCTCTCTCTCTCTCTCTCT 155 25 0 48.00 61.070 0.00 0.00 0.00 6.070 197 TCTCTCTCTCTCTCTCTCTCTCTCT 153 25 0 48.00 61.070 0.00 0.00 0.00 6.070 198 TCTCTCTCTCTCTCTCTCTCTCTCT 151 25 0 48.00 61.070 0.00 0.00 0.00 6.070 199 TCTCTCTCTCTCTCTCTCTCTCTCT 149 25 0 48.00 61.070 0.00 0.00 0.00 6.070 200 TCTCTCTCTCTCTCTCTCTCTCTCT 147 25 0 48.00 61.070 0.00 0.00 0.00 6.070 201 TCTCTCTCTCTCTCTCTCTCTCTCT 145 25 0 48.00 61.070 0.00 0.00 0.00 6.070 202 TCTCTCTCTCTCTCTCTCTCTCTCT 143 25 0 48.00 61.070 0.00 0.00 0.00 6.070 203 TCTCTCTCTCTCTCTCTCTCTCTCT 141 25 0 48.00 61.070 0.00 0.00 0.00 6.070 204 TCTCTCTCTCTCTCTCTCTCTCTCT 139 25 0 48.00 61.070 0.00 0.00 0.00 6.070 205 TCTCTCTCTCTCTCTCTCTCTCTCT 137 25 0 48.00 61.070 0.00 0.00 0.00 6.070 206 TCTCTCTCTCTCTCTCTCTCTCTCT 135 25 0 48.00 61.070 0.00 0.00 0.00 6.070 207 TCTCTCTCTCTCTCTCTCTCTCTCT 133 25 0 48.00 61.070 0.00 0.00 0.00 6.070 208 TCTCTCTCTCTCTCTCTCTCTCTCT 131 25 0 48.00 61.070 0.00 0.00 0.00 6.070 209 TCTCTCTCTCTCTCTCTCTCTCTCT 129 25 0 48.00 61.070 0.00 0.00 0.00 6.070 210 TCTCTCTCTCTCTCTCTCTCTCTCT 127 25 0 48.00 61.070 0.00 0.00 0.00 6.070 211 TCTCTCTCTCTCTCTCTCTCTCTCT 125 25 0 48.00 61.070 0.00 0.00 0.00 6.070 212 TCTCTCTCTCTCTCTCTCTCTCTCT 123 25 0 48.00 61.070 0.00 0.00 0.00 6.070 213 TCTCTCTCTCTCTCTCTCTCTCTCT 121 25 0 48.00 61.070 0.00 0.00 0.00 6.070 214 TCTCTCTCTCTCTCTCTCTCTCTCT 119 25 0 48.00 61.070 0.00 0.00 0.00 6.070 215 TCTCTCTCTCTCTCTCTCTCTCTCT 117 25 0 48.00 61.070 0.00 0.00 0.00 6.070 216 TCTCTCTCTCTCTCTCTCTCTCTCT 115 25 0 48.00 61.070 0.00 0.00 0.00 6.070 217 TCTCTCTCTCTCTCTCTCTCTCTCT 113 25 0 48.00 61.070 0.00 0.00 0.00 6.070 218 TCTCTCTCTCTCTCTCTCTCTCTCT 111 25 0 48.00 61.070 0.00 0.00 0.00 6.070 219 TCTCTCTCTCTCTCTCTCTCTCTCT 109 25 0 48.00 61.070 0.00 0.00 0.00 6.070 220 TCTCTCTCTCTCTCTCTCTCTCTCT 107 25 0 48.00 61.070 0.00 0.00 0.00 6.070 221 TCTCTCTCTCTCTCTCTCTCTCTCT 105 25 0 48.00 61.070 0.00 0.00 0.00 6.070 222 TCTCTCTCTCTCTCTCTCTCTCTCT 103 25 0 48.00 61.070 0.00 0.00 0.00 6.070 223 TCTCTCTCTCTCTCTCTCTCTCTCT 101 25 0 48.00 61.070 0.00 0.00 0.00 6.070 224 TCTCTCTCTCTCTCTCTCTCTCTCTC 155 26 0 50.00 61.874 0.00 0.00 0.00 7.874 225 TCTCTCTCTCTCTCTCTCTCTCTCTC 153 26 0 50.00 61.874 0.00 0.00 0.00 7.874 226 TCTCTCTCTCTCTCTCTCTCTCTCTC 151 26 0 50.00 61.874 0.00 0.00 0.00 7.874 227 TCTCTCTCTCTCTCTCTCTCTCTCTC 149 26 0 50.00 61.874 0.00 0.00 0.00 7.874 228 TCTCTCTCTCTCTCTCTCTCTCTCTC 147 26 0 50.00 61.874 0.00 0.00 0.00 7.874 229 TCTCTCTCTCTCTCTCTCTCTCTCTC 145 26 0 50.00 61.874 0.00 0.00 0.00 7.874 230 TCTCTCTCTCTCTCTCTCTCTCTCTC 143 26 0 50.00 61.874 0.00 0.00 0.00 7.874 231 TCTCTCTCTCTCTCTCTCTCTCTCTC 141 26 0 50.00 61.874 0.00 0.00 0.00 7.874 232 TCTCTCTCTCTCTCTCTCTCTCTCTC 139 26 0 50.00 61.874 0.00 0.00 0.00 7.874 233 TCTCTCTCTCTCTCTCTCTCTCTCTC 137 26 0 50.00 61.874 0.00 0.00 0.00 7.874 234 TCTCTCTCTCTCTCTCTCTCTCTCTC 135 26 0 50.00 61.874 0.00 0.00 0.00 7.874 235 TCTCTCTCTCTCTCTCTCTCTCTCTC 133 26 0 50.00 61.874 0.00 0.00 0.00 7.874 236 TCTCTCTCTCTCTCTCTCTCTCTCTC 131 26 0 50.00 61.874 0.00 0.00 0.00 7.874 237 TCTCTCTCTCTCTCTCTCTCTCTCTC 129 26 0 50.00 61.874 0.00 0.00 0.00 7.874 238 TCTCTCTCTCTCTCTCTCTCTCTCTC 127 26 0 50.00 61.874 0.00 0.00 0.00 7.874 239 TCTCTCTCTCTCTCTCTCTCTCTCTC 125 26 0 50.00 61.874 0.00 0.00 0.00 7.874 240 TCTCTCTCTCTCTCTCTCTCTCTCTC 123 26 0 50.00 61.874 0.00 0.00 0.00 7.874 241 TCTCTCTCTCTCTCTCTCTCTCTCTC 121 26 0 50.00 61.874 0.00 0.00 0.00 7.874 242 TCTCTCTCTCTCTCTCTCTCTCTCTC 119 26 0 50.00 61.874 0.00 0.00 0.00 7.874 243 TCTCTCTCTCTCTCTCTCTCTCTCTC 117 26 0 50.00 61.874 0.00 0.00 0.00 7.874 244 TCTCTCTCTCTCTCTCTCTCTCTCTC 115 26 0 50.00 61.874 0.00 0.00 0.00 7.874 245 TCTCTCTCTCTCTCTCTCTCTCTCTC 113 26 0 50.00 61.874 0.00 0.00 0.00 7.874 246 TCTCTCTCTCTCTCTCTCTCTCTCTC 111 26 0 50.00 61.874 0.00 0.00 0.00 7.874 247 TCTCTCTCTCTCTCTCTCTCTCTCTC 109 26 0 50.00 61.874 0.00 0.00 0.00 7.874 248 TCTCTCTCTCTCTCTCTCTCTCTCTC 107 26 0 50.00 61.874 0.00 0.00 0.00 7.874 249 TCTCTCTCTCTCTCTCTCTCTCTCTC 105 26 0 50.00 61.874 0.00 0.00 0.00 7.874 250 TCTCTCTCTCTCTCTCTCTCTCTCTC 103 26 0 50.00 61.874 0.00 0.00 0.00 7.874 251 TCTCTCTCTCTCTCTCTCTCTCTCTC 101 26 0 50.00 61.874 0.00 0.00 0.00 7.874 252 CTCTCTCTCTCTCTCTCTCTCTCTCT 154 26 0 50.00 61.877 0.00 0.00 0.00 7.877 253 CTCTCTCTCTCTCTCTCTCTCTCTCT 152 26 0 50.00 61.877 0.00 0.00 0.00 7.877 254 CTCTCTCTCTCTCTCTCTCTCTCTCT 150 26 0 50.00 61.877 0.00 0.00 0.00 7.877 255 CTCTCTCTCTCTCTCTCTCTCTCTCT 148 26 0 50.00 61.877 0.00 0.00 0.00 7.877 256 CTCTCTCTCTCTCTCTCTCTCTCTCT 146 26 0 50.00 61.877 0.00 0.00 0.00 7.877 257 CTCTCTCTCTCTCTCTCTCTCTCTCT 144 26 0 50.00 61.877 0.00 0.00 0.00 7.877 258 CTCTCTCTCTCTCTCTCTCTCTCTCT 142 26 0 50.00 61.877 0.00 0.00 0.00 7.877 259 CTCTCTCTCTCTCTCTCTCTCTCTCT 140 26 0 50.00 61.877 0.00 0.00 0.00 7.877 260 CTCTCTCTCTCTCTCTCTCTCTCTCT 138 26 0 50.00 61.877 0.00 0.00 0.00 7.877 261 CTCTCTCTCTCTCTCTCTCTCTCTCT 136 26 0 50.00 61.877 0.00 0.00 0.00 7.877 262 CTCTCTCTCTCTCTCTCTCTCTCTCT 134 26 0 50.00 61.877 0.00 0.00 0.00 7.877 263 CTCTCTCTCTCTCTCTCTCTCTCTCT 132 26 0 50.00 61.877 0.00 0.00 0.00 7.877 264 CTCTCTCTCTCTCTCTCTCTCTCTCT 130 26 0 50.00 61.877 0.00 0.00 0.00 7.877 265 CTCTCTCTCTCTCTCTCTCTCTCTCT 128 26 0 50.00 61.877 0.00 0.00 0.00 7.877 266 CTCTCTCTCTCTCTCTCTCTCTCTCT 126 26 0 50.00 61.877 0.00 0.00 0.00 7.877 267 CTCTCTCTCTCTCTCTCTCTCTCTCT 124 26 0 50.00 61.877 0.00 0.00 0.00 7.877 268 CTCTCTCTCTCTCTCTCTCTCTCTCT 122 26 0 50.00 61.877 0.00 0.00 0.00 7.877 269 CTCTCTCTCTCTCTCTCTCTCTCTCT 120 26 0 50.00 61.877 0.00 0.00 0.00 7.877 270 CTCTCTCTCTCTCTCTCTCTCTCTCT 118 26 0 50.00 61.877 0.00 0.00 0.00 7.877 271 CTCTCTCTCTCTCTCTCTCTCTCTCT 116 26 0 50.00 61.877 0.00 0.00 0.00 7.877 272 CTCTCTCTCTCTCTCTCTCTCTCTCT 114 26 0 50.00 61.877 0.00 0.00 0.00 7.877 273 CTCTCTCTCTCTCTCTCTCTCTCTCT 112 26 0 50.00 61.877 0.00 0.00 0.00 7.877 274 CTCTCTCTCTCTCTCTCTCTCTCTCT 110 26 0 50.00 61.877 0.00 0.00 0.00 7.877 275 CTCTCTCTCTCTCTCTCTCTCTCTCT 108 26 0 50.00 61.877 0.00 0.00 0.00 7.877 276 CTCTCTCTCTCTCTCTCTCTCTCTCT 106 26 0 50.00 61.877 0.00 0.00 0.00 7.877 277 CTCTCTCTCTCTCTCTCTCTCTCTCT 104 26 0 50.00 61.877 0.00 0.00 0.00 7.877 278 CTCTCTCTCTCTCTCTCTCTCTCTCT 102 26 0 50.00 61.877 0.00 0.00 0.00 7.877 279 CTCTCTCTCTCTCTCTCTCTCTCTCT 100 26 0 50.00 61.877 0.00 0.00 0.00 7.877 280 CTCTCTCTCTCTCTCTCTCTCTCTCTC 154 27 0 51.85 62.605 0.00 0.00 0.00 9.605 281 CTCTCTCTCTCTCTCTCTCTCTCTCTC 152 27 0 51.85 62.605 0.00 0.00 0.00 9.605 282 CTCTCTCTCTCTCTCTCTCTCTCTCTC 150 27 0 51.85 62.605 0.00 0.00 0.00 9.605 283 CTCTCTCTCTCTCTCTCTCTCTCTCTC 148 27 0 51.85 62.605 0.00 0.00 0.00 9.605 284 CTCTCTCTCTCTCTCTCTCTCTCTCTC 146 27 0 51.85 62.605 0.00 0.00 0.00 9.605 285 CTCTCTCTCTCTCTCTCTCTCTCTCTC 144 27 0 51.85 62.605 0.00 0.00 0.00 9.605 286 CTCTCTCTCTCTCTCTCTCTCTCTCTC 142 27 0 51.85 62.605 0.00 0.00 0.00 9.605 287 CTCTCTCTCTCTCTCTCTCTCTCTCTC 140 27 0 51.85 62.605 0.00 0.00 0.00 9.605 288 CTCTCTCTCTCTCTCTCTCTCTCTCTC 138 27 0 51.85 62.605 0.00 0.00 0.00 9.605 289 CTCTCTCTCTCTCTCTCTCTCTCTCTC 136 27 0 51.85 62.605 0.00 0.00 0.00 9.605 290 CTCTCTCTCTCTCTCTCTCTCTCTCTC 134 27 0 51.85 62.605 0.00 0.00 0.00 9.605 291 CTCTCTCTCTCTCTCTCTCTCTCTCTC 132 27 0 51.85 62.605 0.00 0.00 0.00 9.605 292 CTCTCTCTCTCTCTCTCTCTCTCTCTC 130 27 0 51.85 62.605 0.00 0.00 0.00 9.605 293 CTCTCTCTCTCTCTCTCTCTCTCTCTC 128 27 0 51.85 62.605 0.00 0.00 0.00 9.605 294 CTCTCTCTCTCTCTCTCTCTCTCTCTC 126 27 0 51.85 62.605 0.00 0.00 0.00 9.605 295 CTCTCTCTCTCTCTCTCTCTCTCTCTC 124 27 0 51.85 62.605 0.00 0.00 0.00 9.605 296 CTCTCTCTCTCTCTCTCTCTCTCTCTC 122 27 0 51.85 62.605 0.00 0.00 0.00 9.605 297 CTCTCTCTCTCTCTCTCTCTCTCTCTC 120 27 0 51.85 62.605 0.00 0.00 0.00 9.605 298 CTCTCTCTCTCTCTCTCTCTCTCTCTC 118 27 0 51.85 62.605 0.00 0.00 0.00 9.605 299 CTCTCTCTCTCTCTCTCTCTCTCTCTC 116 27 0 51.85 62.605 0.00 0.00 0.00 9.605 300 CTCTCTCTCTCTCTCTCTCTCTCTCTC 114 27 0 51.85 62.605 0.00 0.00 0.00 9.605 301 CTCTCTCTCTCTCTCTCTCTCTCTCTC 112 27 0 51.85 62.605 0.00 0.00 0.00 9.605 302 CTCTCTCTCTCTCTCTCTCTCTCTCTC 110 27 0 51.85 62.605 0.00 0.00 0.00 9.605 303 CTCTCTCTCTCTCTCTCTCTCTCTCTC 108 27 0 51.85 62.605 0.00 0.00 0.00 9.605 304 CTCTCTCTCTCTCTCTCTCTCTCTCTC 106 27 0 51.85 62.605 0.00 0.00 0.00 9.605 305 CTCTCTCTCTCTCTCTCTCTCTCTCTC 104 27 0 51.85 62.605 0.00 0.00 0.00 9.605 306 CTCTCTCTCTCTCTCTCTCTCTCTCTC 102 27 0 51.85 62.605 0.00 0.00 0.00 9.605 307 CTCTCTCTCTCTCTCTCTCTCTCTCTC 100 27 0 51.85 62.605 0.00 0.00 0.00 9.605 308 TCTCTCTCTCTCTCTCTCTCTCTCTCT 155 27 0 48.15 62.991 0.00 0.00 0.00 9.991 309 TCTCTCTCTCTCTCTCTCTCTCTCTCT 153 27 0 48.15 62.991 0.00 0.00 0.00 9.991 310 TCTCTCTCTCTCTCTCTCTCTCTCTCT 151 27 0 48.15 62.991 0.00 0.00 0.00 9.991 311 TCTCTCTCTCTCTCTCTCTCTCTCTCT 149 27 0 48.15 62.991 0.00 0.00 0.00 9.991 312 TCTCTCTCTCTCTCTCTCTCTCTCTCT 147 27 0 48.15 62.991 0.00 0.00 0.00 9.991 313 TCTCTCTCTCTCTCTCTCTCTCTCTCT 145 27 0 48.15 62.991 0.00 0.00 0.00 9.991 314 TCTCTCTCTCTCTCTCTCTCTCTCTCT 143 27 0 48.15 62.991 0.00 0.00 0.00 9.991 315 TCTCTCTCTCTCTCTCTCTCTCTCTCT 141 27 0 48.15 62.991 0.00 0.00 0.00 9.991 316 TCTCTCTCTCTCTCTCTCTCTCTCTCT 139 27 0 48.15 62.991 0.00 0.00 0.00 9.991 317 TCTCTCTCTCTCTCTCTCTCTCTCTCT 137 27 0 48.15 62.991 0.00 0.00 0.00 9.991 318 TCTCTCTCTCTCTCTCTCTCTCTCTCT 135 27 0 48.15 62.991 0.00 0.00 0.00 9.991 319 TCTCTCTCTCTCTCTCTCTCTCTCTCT 133 27 0 48.15 62.991 0.00 0.00 0.00 9.991 320 TCTCTCTCTCTCTCTCTCTCTCTCTCT 131 27 0 48.15 62.991 0.00 0.00 0.00 9.991 321 TCTCTCTCTCTCTCTCTCTCTCTCTCT 129 27 0 48.15 62.991 0.00 0.00 0.00 9.991 322 TCTCTCTCTCTCTCTCTCTCTCTCTCT 127 27 0 48.15 62.991 0.00 0.00 0.00 9.991 323 TCTCTCTCTCTCTCTCTCTCTCTCTCT 125 27 0 48.15 62.991 0.00 0.00 0.00 9.991 324 TCTCTCTCTCTCTCTCTCTCTCTCTCT 123 27 0 48.15 62.991 0.00 0.00 0.00 9.991 325 TCTCTCTCTCTCTCTCTCTCTCTCTCT 121 27 0 48.15 62.991 0.00 0.00 0.00 9.991 326 TCTCTCTCTCTCTCTCTCTCTCTCTCT 119 27 0 48.15 62.991 0.00 0.00 0.00 9.991 327 TCTCTCTCTCTCTCTCTCTCTCTCTCT 117 27 0 48.15 62.991 0.00 0.00 0.00 9.991 328 TCTCTCTCTCTCTCTCTCTCTCTCTCT 115 27 0 48.15 62.991 0.00 0.00 0.00 9.991 329 TCTCTCTCTCTCTCTCTCTCTCTCTCT 113 27 0 48.15 62.991 0.00 0.00 0.00 9.991 330 TCTCTCTCTCTCTCTCTCTCTCTCTCT 111 27 0 48.15 62.991 0.00 0.00 0.00 9.991 331 TCTCTCTCTCTCTCTCTCTCTCTCTCT 109 27 0 48.15 62.991 0.00 0.00 0.00 9.991 332 TCTCTCTCTCTCTCTCTCTCTCTCTCT 107 27 0 48.15 62.991 0.00 0.00 0.00 9.991 333 TCTCTCTCTCTCTCTCTCTCTCTCTCT 105 27 0 48.15 62.991 0.00 0.00 0.00 9.991 334 TCTCTCTCTCTCTCTCTCTCTCTCTCT 103 27 0 48.15 62.991 0.00 0.00 0.00 9.991 335 TCTCTCTCTCTCTCTCTCTCTCTCTCT 101 27 0 48.15 62.991 0.00 0.00 0.00 9.991 primer3-2.3.6/test/primer1_th_list_last/SP01-06.txt.rev0000664000077300007730000003105312231156243021427 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 CGTGCTTTGCGTTTTGGAAG 127 20 0 50.00 60.034 0.00 0.00 0.00 0.034 1 GTGCTTTGCGTTTTGGAAGT 126 20 0 45.00 58.977 0.00 0.00 0.00 1.023 2 GAGCGTGCTTTGCGTTTTG 130 19 0 52.63 60.026 0.00 0.00 37.77 1.026 3 GTGCTTTGCGTTTTGGAAGTT 126 21 0 42.86 59.636 0.00 0.00 0.00 1.364 4 TGCTTTGCGTTTTGGAAGTT 125 20 0 40.00 58.246 0.00 0.00 0.00 1.754 5 GCGTGCTTTGCGTTTTGGAA 128 20 0 50.00 62.034 0.00 0.00 0.00 2.034 6 GCGTGCTTTGCGTTTTGG 128 18 0 55.56 59.941 0.00 0.00 0.00 2.059 7 CGTGCTTTGCGTTTTGGAA 127 19 0 47.37 58.931 0.00 0.00 0.00 2.069 8 GGAGCGTGCTTTGCGTTTT 131 19 0 52.63 61.186 0.00 0.00 37.77 2.186 9 GATCGGGAGCGTGCTTTG 136 18 0 61.11 59.784 0.00 0.00 0.00 2.216 10 GGAGCGTGCTTTGCGTTTTG 131 20 0 55.00 62.422 0.00 0.00 37.77 2.422 11 GAGCGTGCTTTGCGTTTTGG 130 20 0 55.00 62.422 0.00 0.00 37.77 2.422 12 GCGTGCTTTGCGTTTTGGA 128 19 0 52.63 61.472 0.00 0.00 0.00 2.472 13 AGCGTGCTTTGCGTTTTGG 129 19 0 52.63 61.476 0.00 0.00 37.77 2.476 14 GTGCTTTGCGTTTTGGAAGTTA 126 22 0 40.91 59.468 0.00 0.00 0.00 2.532 15 GGAGCGTGCTTTGCGTTT 131 18 0 55.56 60.548 0.00 0.00 37.77 2.548 16 CGTGCTTTGCGTTTTGGAAGT 127 21 0 47.62 61.698 0.00 0.00 0.00 2.698 17 TGCTTTGCGTTTTGGAAGTTAG 125 22 0 40.91 59.209 0.00 0.00 0.00 2.791 18 TGCTTTGCGTTTTGGAAGTTA 125 21 0 38.10 58.130 0.00 0.00 0.00 2.870 19 AGCGTGCTTTGCGTTTTGGA 129 20 0 50.00 62.908 0.00 0.00 37.77 2.908 20 GCTTTGCGTTTTGGAAGTTAGA 124 22 0 40.91 58.951 0.00 0.00 0.00 3.049 21 AGCGTGCTTTGCGTTTTG 129 18 0 50.00 58.874 0.00 0.00 37.77 3.126 22 GCTTTGCGTTTTGGAAGTTAGAC 124 23 0 43.48 60.176 0.00 0.00 0.00 3.176 23 GTGCTTTGCGTTTTGGAAGTTAG 126 23 0 43.48 60.419 0.00 0.00 0.00 3.419 24 GAGCGTGCTTTGCGTTTT 130 18 0 50.00 58.568 0.00 0.00 37.77 3.432 25 GCTTTGCGTTTTGGAAGTTAG 124 21 0 42.86 57.515 0.00 0.00 0.00 3.485 26 TGCTTTGCGTTTTGGAAGT 125 19 0 42.11 57.471 0.00 0.00 0.00 3.529 27 TGCTTTGCGTTTTGGAAGTTAGA 125 23 0 39.13 60.550 0.00 0.00 0.00 3.550 28 CGTGCTTTGCGTTTTGGA 127 18 0 50.00 58.175 0.00 0.00 0.00 3.825 29 GTGCTTTGCGTTTTGGAAG 126 19 0 47.37 57.116 0.00 0.00 0.00 3.884 30 GCGTGCTTTGCGTTTTGGAAG 128 21 0 52.38 62.910 0.00 0.00 0.00 3.910 31 GATCGGGAGCGTGCTTTGC 136 19 0 63.16 62.988 0.00 0.00 0.00 3.988 32 TGCGTTTTGGAAGTTAGACTTTG 120 23 0 39.13 59.001 0.00 0.00 39.75 3.999 33 ATCGGGAGCGTGCTTTGC 135 18 0 61.11 62.020 0.00 0.00 0.00 4.020 34 GCGTTTTGGAAGTTAGACTTTGAC 119 24 0 41.67 59.943 0.00 0.00 39.75 4.057 35 CGTGCTTTGCGTTTTGGAAGTT 127 22 0 45.45 62.204 0.00 0.00 0.00 4.204 36 GCGTTTTGGAAGTTAGACTTTGA 119 23 0 39.13 58.752 0.00 0.00 39.75 4.248 37 CTTTGCGTTTTGGAAGTTAGACT 123 23 0 39.13 58.749 0.00 0.00 0.00 4.251 38 TGCGTTTTGGAAGTTAGACTTTGA 120 24 0 37.50 60.297 0.00 0.00 39.75 4.297 39 TTTGCGTTTTGGAAGTTAGACT 122 22 0 36.36 57.684 0.00 0.00 0.00 4.316 40 TTGCGTTTTGGAAGTTAGACTT 121 22 0 36.36 57.684 0.00 0.00 42.62 4.316 41 TGCGTTTTGGAAGTTAGACTTT 120 22 0 36.36 57.684 0.00 0.00 39.75 4.316 42 TTGCGTTTTGGAAGTTAGACTTTG 121 24 0 37.50 59.584 0.00 0.00 39.75 4.416 43 GGGAGCGTGCTTTGCGTT 132 18 0 61.11 62.550 0.00 0.00 37.77 4.550 44 TTGACAACAGACTCATACAGTGA 100 23 0 39.13 58.405 0.00 0.00 35.23 4.595 45 CTTTGCGTTTTGGAAGTTAGAC 123 22 0 40.91 57.367 0.00 0.00 0.00 4.633 46 GCGTTTTGGAAGTTAGACTTTG 119 22 0 40.91 57.367 0.00 0.00 39.75 4.633 47 TTTGCGTTTTGGAAGTTAGACTT 122 23 0 34.78 58.357 0.00 0.00 42.62 4.643 48 TTGCGTTTTGGAAGTTAGACTTT 121 23 0 34.78 58.357 0.00 0.00 39.75 4.643 49 CTTTGCGTTTTGGAAGTTAGACTT 123 24 0 37.50 59.344 0.00 0.00 42.62 4.656 50 CGTGCTTTGCGTTTTGGAAGTTA 127 23 0 43.48 61.942 0.00 0.00 0.00 4.942 51 TTTGACAACAGACTCATACAGTGA 101 24 0 37.50 59.026 0.00 0.00 35.23 4.974 52 ACTTTGACAACAGACTCATACAGT 103 24 0 37.50 59.023 5.47 0.00 38.87 4.977 53 TTTGCGTTTTGGAAGTTAGACTTT 122 24 0 33.33 58.975 0.00 0.00 39.75 5.025 54 TGGAAGTTAGACTTTGACAACAGA 113 24 0 37.50 58.959 5.47 0.00 39.75 5.041 55 CTTTGACAACAGACTCATACAGTGA 102 25 0 40.00 59.948 0.00 0.00 35.23 5.052 56 GACTTTGACAACAGACTCATACAGT 104 25 0 40.00 59.948 0.00 0.00 38.87 5.052 57 CTTTGCGTTTTGGAAGTTAGACTTT 123 25 0 36.00 59.893 0.00 0.00 39.75 5.107 58 GGAAGTTAGACTTTGACAACAGACT 112 25 0 40.00 59.889 7.87 7.87 39.75 5.111 59 TGGAAGTTAGACTTTGACAACAGAC 113 25 0 40.00 60.121 0.00 0.00 39.75 5.121 60 TTTGCGTTTTGGAAGTTAGACTTTG 122 25 0 36.00 60.122 0.00 0.00 39.75 5.122 61 ACTTTGACAACAGACTCATACAGTG 103 25 0 40.00 60.179 5.47 0.00 35.23 5.179 62 CGTTTTGGAAGTTAGACTTTGACA 118 24 0 37.50 58.808 0.00 0.00 39.75 5.192 63 AGACTTTGACAACAGACTCATACA 105 24 0 37.50 58.779 0.00 0.00 0.00 5.221 64 AGTTAGACTTTGACAACAGACTCA 109 24 0 37.50 58.718 1.42 0.00 0.00 5.282 65 AGACTTTGACAACAGACTCATACAG 105 25 0 40.00 59.716 0.00 0.00 0.00 5.284 66 CTTTGACAACAGACTCATACAGTG 102 24 0 41.67 58.696 0.00 0.00 35.23 5.304 67 TTTGACAACAGACTCATACAGTG 101 23 0 39.13 57.679 0.00 0.00 35.23 5.321 68 GGAAGTTAGACTTTGACAACAGAC 112 24 0 41.67 58.631 0.00 0.00 39.75 5.369 69 TGGAAGTTAGACTTTGACAACAG 113 23 0 39.13 57.603 0.00 0.00 39.75 5.397 70 GCTTTGCGTTTTGGAAGTTAGACT 124 24 0 41.67 61.422 0.00 0.00 0.00 5.422 71 TTGGAAGTTAGACTTTGACAACAGA 114 25 0 36.00 59.535 5.47 0.00 39.75 5.465 72 GACTTTGACAACAGACTCATACAG 104 24 0 41.67 58.458 0.00 0.00 0.00 5.542 73 GACTTTGACAACAGACTCATACA 104 23 0 39.13 57.427 0.00 0.00 0.00 5.573 74 ACTTTGACAACAGACTCATACAG 103 23 0 39.13 57.421 5.47 0.00 0.00 5.579 75 CTTTGACAACAGACTCATACAGT 102 23 0 39.13 57.421 0.00 0.00 38.87 5.579 76 CGTTTTGGAAGTTAGACTTTGACAA 118 25 0 36.00 59.378 1.10 1.10 39.75 5.622 77 GTTAGACTTTGACAACAGACTCA 108 23 0 39.13 57.365 0.00 0.00 0.00 5.635 78 GGAAGTTAGACTTTGACAACAGA 112 23 0 39.13 57.349 5.47 0.00 39.75 5.651 79 GTGCTTTGCGTTTTGGAAGTTAGA 126 24 0 41.67 61.655 0.00 0.00 0.00 5.655 80 TGCTTTGCGTTTTGGAAGTTAGAC 125 24 0 41.67 61.655 0.00 0.00 0.00 5.655 81 AAGTTAGACTTTGACAACAGACTCA 110 25 0 36.00 59.303 1.42 0.00 35.20 5.697 82 TTGGAAGTTAGACTTTGACAACAG 114 24 0 37.50 58.252 3.24 0.00 39.75 5.748 83 CGTTTTGGAAGTTAGACTTTGAC 118 23 0 39.13 57.231 0.00 0.00 39.75 5.769 84 TTGGAAGTTAGACTTTGACAACA 114 23 0 34.78 57.194 3.24 0.00 39.75 5.806 85 TTGCGTTTTGGAAGTTAGACTTTGA 121 25 0 36.00 60.814 0.00 0.00 39.75 5.814 86 AGACTTTGACAACAGACTCATAC 105 23 0 39.13 57.168 4.15 0.00 0.00 5.832 87 AGTTAGACTTTGACAACAGACTCAT 109 25 0 36.00 59.125 1.42 0.00 0.00 5.875 88 AGTTAGACTTTGACAACAGACTC 109 23 0 39.13 57.106 5.50 0.00 0.00 5.894 89 TTTGGAAGTTAGACTTTGACAACAGA 115 26 0 34.62 60.068 5.47 0.00 39.75 6.068 90 TTTGGAAGTTAGACTTTGACAACA 115 24 0 33.33 57.867 0.00 0.00 39.75 6.133 91 TTTGGAAGTTAGACTTTGACAACAG 115 25 0 36.00 58.850 0.99 0.00 39.75 6.150 92 GTTAGACTTTGACAACAGACTCAT 108 24 0 37.50 57.834 0.00 0.00 0.00 6.166 93 GAAGTTAGACTTTGACAACAGACTCA 111 26 0 38.46 60.180 0.58 0.00 39.75 6.180 94 GAAGTTAGACTTTGACAACAGACT 111 24 0 37.50 57.774 7.87 7.87 39.75 6.226 95 AAGTTAGACTTTGACAACAGACTC 110 24 0 37.50 57.774 5.50 0.00 35.20 6.226 96 GAAGTTAGACTTTGACAACAGACTC 111 25 0 40.00 58.753 0.00 0.00 39.75 6.247 97 AAGTTAGACTTTGACAACAGACTCAT 110 26 0 34.62 59.673 1.42 0.00 35.20 6.327 98 TAGACTTTGACAACAGACTCATACA 106 25 0 36.00 58.659 0.00 0.00 0.00 6.341 99 GTTTTGGAAGTTAGACTTTGACAACA 117 26 0 34.62 59.625 2.05 0.00 39.75 6.375 100 TGCGTTTTGGAAGTTAGACTTTGAC 120 25 0 40.00 61.376 0.00 0.00 39.75 6.376 101 GCGTTTTGGAAGTTAGACTTTGACA 119 25 0 40.00 61.376 0.00 0.00 39.75 6.376 102 TAGACTTTGACAACAGACTCATACAG 106 26 0 38.46 59.568 0.00 0.00 0.00 6.432 103 CGTTTTGGAAGTTAGACTTTGACAAC 118 26 0 38.46 60.452 0.72 0.00 39.75 6.452 104 TTTTGGAAGTTAGACTTTGACAACA 116 25 0 32.00 58.486 0.01 0.00 39.75 6.514 105 TTTTGGAAGTTAGACTTTGACAACAG 116 26 0 34.62 59.402 1.12 0.00 39.75 6.598 106 TTGGAAGTTAGACTTTGACAACAGAC 114 26 0 38.46 60.625 3.24 0.00 39.75 6.625 107 CGTGCTTTGCGTTTTGGAAGTTAG 127 24 0 45.83 62.725 0.00 0.00 0.00 6.725 108 GGAAGTTAGACTTTGACAACAGACTC 112 26 0 42.31 60.731 0.00 0.00 39.75 6.731 109 TTAGACTTTGACAACAGACTCATACA 107 26 0 34.62 59.224 0.00 0.00 0.00 6.776 110 GTTTTGGAAGTTAGACTTTGACAAC 117 25 0 36.00 58.183 0.00 0.00 39.75 6.817 111 GCTTTGCGTTTTGGAAGTTAGACTT 124 25 0 40.00 61.888 0.00 0.00 42.62 6.888 112 TAGACTTTGACAACAGACTCATAC 106 24 0 37.50 57.108 0.00 0.00 0.00 6.892 113 CTTTGCGTTTTGGAAGTTAGACTTTG 123 26 0 38.46 60.941 0.00 0.00 39.75 6.941 114 GACTTTGACAACAGACTCATACAGTG 104 26 0 42.31 61.002 0.00 0.00 35.23 7.002 115 AGTTAGACTTTGACAACAGACTCATA 109 26 0 34.62 58.996 1.42 0.00 0.00 7.004 116 AGTTAGACTTTGACAACAGACTCATAC 109 27 0 37.04 60.074 1.70 0.87 0.00 7.074 117 TTAGACTTTGACAACAGACTCATACAG 107 27 0 37.04 60.074 0.00 0.00 0.00 7.074 118 GTTAGACTTTGACAACAGACTCATAC 108 26 0 38.46 58.916 0.00 0.00 0.00 7.084 119 AGACTTTGACAACAGACTCATACAGT 105 26 0 38.46 61.136 0.00 0.00 38.87 7.136 120 GTTAGACTTTGACAACAGACTCATA 108 25 0 36.00 57.751 0.00 0.00 0.00 7.249 121 TTAGACTTTGACAACAGACTCATAC 107 25 0 36.00 57.751 0.00 0.00 0.00 7.249 122 GTTAGACTTTGACAACAGACTCATACA 108 27 0 37.04 60.288 2.57 0.00 0.00 7.288 123 TTTGCGTTTTGGAAGTTAGACTTTGA 122 26 0 34.62 61.290 0.00 0.00 39.75 7.290 124 TGGAAGTTAGACTTTGACAACAGACT 113 26 0 38.46 61.307 7.87 7.87 39.75 7.307 125 ACTTTGACAACAGACTCATACAGTGA 103 26 0 38.46 61.357 5.47 0.00 35.23 7.357 126 GTTTTGGAAGTTAGACTTTGACAACAG 117 27 0 37.04 60.446 2.05 0.00 39.75 7.446 127 AAGTTAGACTTTGACAACAGACTCATA 110 27 0 33.33 59.530 1.42 0.00 35.20 7.470 128 GAAGTTAGACTTTGACAACAGACTCAT 111 27 0 37.04 60.503 0.58 0.00 39.75 7.503 129 TTTTGGAAGTTAGACTTTGACAACAGA 116 27 0 33.33 60.560 5.47 0.00 39.75 7.560 130 GTGCTTTGCGTTTTGGAAGTTAGAC 126 25 0 44.00 62.649 0.00 0.00 0.00 7.649 131 TTGCGTTTTGGAAGTTAGACTTTGAC 121 26 0 38.46 61.826 0.00 0.00 39.75 7.826 132 GCGTTTTGGAAGTTAGACTTTGACAA 119 26 0 38.46 61.826 1.10 1.10 39.75 7.826 133 TGCTTTGCGTTTTGGAAGTTAGACT 125 25 0 40.00 62.829 0.00 0.00 0.00 7.829 134 TAGACTTTGACAACAGACTCATACAGT 106 27 0 37.04 60.940 0.00 0.00 38.87 7.940 135 TTTGGAAGTTAGACTTTGACAACAGAC 115 27 0 37.04 61.092 0.99 0.00 39.75 8.092 136 GCTTTGCGTTTTGGAAGTTAGACTTT 124 26 0 38.46 62.319 0.00 0.00 39.75 8.319 137 TGCGTTTTGGAAGTTAGACTTTGACA 120 26 0 38.46 62.731 0.00 0.00 39.75 8.731 138 TTGGAAGTTAGACTTTGACAACAGACT 114 27 0 37.04 61.754 7.87 7.87 39.75 8.754 139 CGTTTTGGAAGTTAGACTTTGACAACA 118 27 0 37.04 61.772 4.06 0.00 39.75 8.772 140 CTTTGCGTTTTGGAAGTTAGACTTTGA 123 27 0 37.04 62.036 0.00 0.00 39.75 9.036 141 TGGAAGTTAGACTTTGACAACAGACTC 113 27 0 40.74 62.059 0.00 0.00 39.75 9.059 142 GGAAGTTAGACTTTGACAACAGACTCA 112 27 0 40.74 62.059 2.68 0.00 39.75 9.059 143 GACTTTGACAACAGACTCATACAGTGA 104 27 0 40.74 62.103 0.00 0.00 35.23 9.103 144 AGACTTTGACAACAGACTCATACAGTG 105 27 0 40.74 62.107 0.00 0.00 35.23 9.107 145 TTTGCGTTTTGGAAGTTAGACTTTGAC 122 27 0 37.04 62.244 0.00 0.00 39.75 9.244 146 GCGTTTTGGAAGTTAGACTTTGACAAC 119 27 0 40.74 62.744 0.72 0.00 39.75 9.744 primer3-2.3.6/test/primer1_th_list_last/SP01-06.txt.for0000664000077300007730000000217212231156243021421 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TCGCAATCAAATTTTTTCTATGACCT 37 26 0 30.77 59.341 0.00 0.00 0.00 6.659 1 TCGCAATCAAATTTTTTCTATGACC 37 25 0 32.00 58.114 0.00 0.00 0.00 6.886 2 ATCGCAATCAAATTTTTTCTATGACCT 36 27 0 29.63 59.695 0.00 0.00 0.00 7.305 3 TCGCAATCAAATTTTTTCTATGACCTG 37 27 0 33.33 60.394 0.00 0.00 0.00 7.394 4 ATCGCAATCAAATTTTTTCTATGACC 36 26 0 30.77 58.519 0.00 0.00 0.00 7.481 5 AATCGCAATCAAATTTTTTCTATGACC 35 27 0 29.63 59.061 0.00 0.00 0.00 7.939 6 TGAATCGCAATCAAATTTTTTCTATGA 33 27 0 25.93 58.301 0.00 0.00 0.00 8.699 7 TGAATCGCAATCAAATTTTTTCTATG 33 26 0 26.92 57.093 0.00 0.00 0.00 8.907 8 AATCGCAATCAAATTTTTTCTATGAC 35 26 0 26.92 57.092 0.00 0.00 0.00 8.908 9 GAATCGCAATCAAATTTTTTCTATGAC 34 27 0 29.63 58.026 0.00 0.00 0.00 8.974 10 ATGAATCGCAATCAAATTTTTTCTATG 32 27 0 25.93 57.518 0.00 0.00 0.00 9.482 primer3-2.3.6/test/primer_high_tm_load_set_input0000664000077300007730000000326412231156245021053 0ustar ioanaioanaSEQUENCE_ID=Load qPCR settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_TASK=pick_detection_primers = primer3-2.3.6/test/primer_input0000664000077300007730000003236612231156245015507 0ustar ioanaioanaPRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 P3_FILE_FLAG=1 SEQUENCE_ID=targets_test SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 = SEQUENCE_ID=SP01-02.txt SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG = SEQUENCE_ID=SP01-03.txt SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC = SEQUENCE_ID=SP01-06.txt SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC = SEQUENCE_ID=SP01-15.txt SEQUENCE_TEMPLATE=GATCNCCCCTCNNNTGNTCTTTCTGCCANTGACACTCTGGNNATCATGGTTCATNTNTCTTNGAAAATTACTCTCANGACCGGGNCACTGTGTATACGNNGGANNTGTACCCTAGTNTCTGNNGNTAANGGCTTGGANNNTNGGGATNTGTTATTTCCNGAGGNGCNGANGTCGNGGTGGATCATCCANTCGTNCTANAGNGGAGTCNTACCGACGGTGGATCTCTGGNACGTCNTTATAAAAGAGTCNTATTACGCGCGCTCACTGGCCGTCGTTTAAAAAATNNTNNNNNCCCCNCTC = SEQUENCE_ID=SP01-19.txt SEQUENCE_TEMPLATE=GATCANANTTGTTTACNTCTGTTCCTTGGAATATTNCTCAAATATTAATATTCCTGTCCCTGTGCCTCAGAAACCTGGCTACAGTTCCCTCNGAAGNACNGTGGNCNTGTCTTGAGAAGAATCTTCCTANCACGGTTAACCAGAGGGCCAANGTGACACTGGCAGAAAGAGCACTGGATGAGGGATC = SEQUENCE_ID=SP01-20.txt SEQUENCE_TEMPLATE=GATCTCCNCGGTAAGANCTTNNCTCAAAACANTGAACCGANCTGAAATTCNACGTAGNATGGGANAGTNACGGGCCTNCTTTCTACNAAGAAANATNNGAAATCTGGGGCCGTTGGGGTNTCTCTCTCNGGAAAGGGGAAAANTCANGGATTCTTTCTCCNCCNCCCCNACGGATCNAACTCCACCGNGGATNTTTCTGAATGATNTGCCCCGGGCCCCNGGNTCTGCTNTNAAAAAATC = SEQUENCE_ID=SP01-21.txt SEQUENCE_TEMPLATE=GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC = SEQUENCE_ID=SP03-40.txt SEQUENCE_TEMPLATE=GANCACCTCTGAAATAACGCCATTCACCAGCTCTCTGGGGCCTGCGAGTCTCTGCCTTCATCCTCACGGGTCTACGAGGCAACATGATC = SEQUENCE_ID=SP04-07.txt SEQUENCE_TEMPLATE=GATCACACTCACTGAAGACTTCCCTACATACAGCCAAGGGACTGCCACCGTGTACACACTGAACACGTTCCCTAAGAAATGTGGTTAAGGGACCGCCGCCATGTACACACTGAAGATGTTCCCTAAGAATGTGGCCAAGGGAATGCACTGAAGACCTTCCCTAATACAAAAGCTAAGACATTGTGGGTGTACCACACACTTCCTACAGCTGTGGGAAAATGAGGCTCTTCAGGGCACCTCCATGACTAGCCACAAACCCAGCTAGGAGAGCCACGGAAAGAATGGGGTGGTGTAACTGCA = SEQUENCE_ID=AGAG SEQUENCE_TEMPLATE=AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA = SEQUENCE_ID=long_seq SEQUENCE_TEMPLATE=aaagcatcggattagctagctgatgctagtgatgcatgctagtagtgatgtatacgtagctagtagctagctagctagctgatcgatcgatgtgcggctagctagctagctagctgactgatcgatcgatgctagctagtagctagctagctagctgatcatcatctagctagtagctttatgctagctagctagctgatcatcgatgctactagctagctgatcatcatctactatcatcatcatctactagctatttagctagctgactgatcgatcatcatgctagctactagctagctagctagctactgatcgatgctacatcgatcgatgctagtatgctagctagctagctactatcatctctgcgcgatcgatgctagctagctagctagctgatcgatcgatgctagctaggcgatgctagctagctactatcatcgatcgatgcatgcatgctactagctagctgactgatacgcgatgctagctagctagctagctactagctagctgatcatcatcgatgctagctagtagctatcgatcatcgatgctagctagctgatcgatcgtagcggcgatctagctagctgactagctgatcgatcgtagctagctagtcgatcatgctagctagctagctagctagctgacgatgctagctagctgggctactagctagcgcatcg = SEQUENCE_ID=bad_letter SEQUENCE_TEMPLATE=AAACNACACAGCTAGCTAGCTATCGTAGCTACTAGCACACAGCTACGTACGACTAGCTACACACNACTAGCGATCATCTACTAGCANATTAGCAGCTACGACTAGCATCGACTAGCATCGACTACTAGCTAGCTAGCGGCGTACTAGCTAGCTAGCTTACGTAGCTACTATCATCGATGCTAGCKATCGATCGTAGCTAGCTAGTCANACTACGATCGATCGTAGC = SEQUENCE_ID=bad_target SEQUENCE_TEMPLATE=AAGCGATCGGGCGCTACGGACGATCATCTGACTGATCGGACTGACTGAGGGCGTAGCTAGCTAGCTGACATGCTAGCTAGCTAGCGATCGTAGCTACCGGCTATCAGCTAGCTGACGCTAGCTAGCGATCGATGCAGCTTATGCAGCTAGCTAGCGACGGCGCGCTACGTAGCTAGCTAGCTAGCTAGCTGACTGATGCTGACTTATCGTACGTAGCTAGCTGACTGATGCTAGCTAGCTACTAGCTACTAC SEQUENCE_TARGET=50,10 SEQUENCE_TARGET=500,50 = SEQUENCE_ID=other_bad_target SEQUENCE_TEMPLATE=AGAGCTATCATGCTAGCTAGCTGATCTAGCTAGCTGATCGATCTACTAGCTAGTCGATCGTAGCTAGCTAGCTGATGCTACTACTAGCTGATCTAGCTAGCTATCATGCTACTATCATCAGCTGATCATCTACTAGTCATCTAGCTATCATCGATGCTAGCTACTACTAGTCGATCTAGCTAGGCGGCTACTAGCTAGCTATCTAGCTAGCTGATCACATCGTAGCTAGCTAGCTGATCGATGCTACTAGTCATCTAGCTAGCTAGC SEQUENCE_TARGET=100,1000 = SEQUENCE_ID=included_test SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=20,228 = SEQUENCE_ID=included_test1 SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=20-200 = PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MAX_SELF_END=8 SEQUENCE_ID=included_test_w_middle SEQUENCE_TEMPLATE=AACGTAGCATGTGCGATGCTGAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTCNCTGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATCAAAGCTAGCTAGCTGATGTCGTAGCTGTAGCTGATCGTCTACGAGG SEQUENCE_INCLUDED_REGION=20,228 = SEQUENCE_ID=long_primer_lists PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 P3_FILE_FLAG=0 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=20 PRIMER_PAIR_MAX_COMPL_ANY=20 PRIMER_PAIR_MAX_COMPL_END=20 SEQUENCE_TEMPLATE=TTTTTTTTATTATTATACTTCAAGTTTTAGGGTACATGTGCACAACGGCAGGTTTGTTATATATGTATACATGTGCCATGTTGGTGTGCTGCACCCATTAACTCGTCATTTAGCATTAGGTATATCTCCTAGTGCTATCCCTGCCCCTGCCCCCCACCCCATAACAGTCCCCAGAGTGTGATGTTCCTCTTCATGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGTGAGAACATGCGGTGTTTGGTTTATTGTCCTTGTGATAGTTTGCTGAGAATGATAGTTTCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAATCTATCGTTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATTGTGAATAGTGCTGCAATAAACATACGTGTGCATGTGTCTTTATAGCAGCATGATTTATAATCCTTTGGGTATCTACCCAGTAATGGGATGGCTGGGTCAAATGGTATTTCTAGTTCTAGATTCCTGAGGAATTGCCACACTGACTTCCGCAATGGTTGAACTAGTTAATTAGATCCCGTTTGTCC = SEQUENCE_ID=end_complementarity_failure PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=2 PRIMER_PAIR_MAX_COMPL_ANY=20 PRIMER_PAIR_MAX_COMPL_END=2 PRIMER_INTERNAL_MAX_SELF_END=2 SEQUENCE_TEMPLATE=TTTTTTTTATTATTATACTTCAAGTTTTAGGGTACATGTGCACAACGGCAGGTTTGTTATATATGTATACATGTGCCATGTTGGTGTGCTGCACCCATTAACTCGTCATTTAGCATTAGGTATATCTCCTAGTGCTATCCCTGCCCCTGCCCCCCACCCCATAACAGTCCCCAGAGTGTGATGTTCCTCTTCATGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGTGAGAACATGCGGTGTTTGGTTTATTGTCCTTGTGATAGTTTGCTGAGAATGATAGTTTCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCC = SEQUENCE_TEMPLATE=GATCGATCGATGCTAGCTGACTGATCGTAGCTAGTCGATCGTAGCTAGCTATCGTACTACTATCATCTAGCTACTGATCGATGGGGCTGTGCTTCGATGCGGTGACGGCTAGCTAGCTGATCGGCTAGCTGACGCTGATCGATGCTATACTACATCTACTACTACGATCACTGGGCATCGTAGCTAGCTACACATCTAGCTGACAGC P3_FILE_FLAG=1 = SEQUENCE_ID=bad_included_region SEQUENCE_TEMPLATE=ATCTGCTAGCTGATCGAGCTATCATGCTAGCTGATCATGCTAGCTATCGTACTACTATCATCTACTGATCATGCTAGCTACTATCATCTACTATCATCATCTAGCTACTAGCTACTACGATCTACTACTATCATCTACTAGCTACTATCACTACTACTATCATCATCTACTACTACATCACACTGATCGTGGGCTAGCTGGCAGCGCG SEQUENCE_INCLUDED_REGION=-1,100 P3_FILE_FLAG=0 = SEQUENCE_ID=bad_included_region1 SEQUENCE_TEMPLATE=GTAGCAGCTAGCTAGCTAGTCGATCGTAGCTAGCTAGTCATCTACGTAGCTAGCTAGTCATCGTAGCTGATCGATGCTAGCTAGCTGATCGGGCGACTGTTCATATGATGGGCTACTGACTGATCGTAGCTGGCGATCGTAGCGAGGCGATCTAGCTAGCTAGCTATCGGCTACTGATCGTACTGACTGATGCTAGCTAGGGCGTAGCTAG SEQUENCE_INCLUDED_REGION=150,-90 = SEQUENCE_ID=SP01-06-with-repeat1 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MISPRIMING_LIBRARY=../not_for_production.lib PRIMER_MAX_LIBRARY_MISPRIMING=14 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC = SEQUENCE_ID=SP01-06-with-repeat2 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MISPRIMING_LIBRARY=../not_for_production.lib PRIMER_MAX_LIBRARY_MISPRIMING=14 PRIMER_INTERNAL_MISHYB_LIBRARY=../not_for_production.lib PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=14 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC = SEQUENCE_ID=SP01-06-with-repeat3 P3_FILE_FLAG=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MISPRIMING_LIBRARY= PRIMER_INTERNAL_MISHYB_LIBRARY=../not_for_production.lib PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=14 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC = SEQUENCE_ID=case_problem_test PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MISPRIMING_LIBRARY= PRIMER_INTERNAL_MISHYB_LIBRARY= SEQUENCE_TEMPLATE=atggccaaagccgcggcagtcggcatcgacctgggcaccacctactcctgcgtgggggtgttccaacacggcaaggtggagatcatcgccaacgaccagggcaaccgcaccacccccagctacgtggccttcacggacaccgagcggctcatcggggatgcggccaagaaccaggtggcgctgaacccgcagaacaccgtgtttgacgcgaagcgcctgatcggccgcaagttcggcgacccggtggtgcagtcggacatgaagcactggcctttccaggtgatcaacgacggagacaagcccaaggtgcaggtgagctacaagggggagaccaaggcattctaccccgaggagatctcgtccatggtgctgaccaagatgaaggagatcgccgaggcgtacctgggctacccggtgaccaacgcggtgatcaccgtgccggcctacttcaacgactcgcagcgccaggccaccaaggatgcgggtgtgatcgcggggctcaacgtgctgcggatcatcaacgagcccacggccgccgccatcgcctacggcctggacagaacgggcaagggggagcgcaacgtcctgatctttgacctgggcgggggcaccttcgacgtgtccatcctgacgatcgacgacggcatcttcgaggtgaaggccacggccggggacacccacctgggtggggaggactttgacaacaggctggtgaaccacttcgtggaggagttcaagagaaaacacaagaaggacatcagccagaacaagcgagccgtgaggcggctgcgcaccgcctgcgagagggccaagaggaccctgtcgtccagcacccaggccagcctggagatcgactccctgtttgagggcatcgacttctacacgtccatcaccagggcgaggttcgaggagctgtgctccgacctgttccgaagcaccctggagcccgtggagaaggctctgcgcgacgccaagctggacaaggcccagattcacgacctggtcctggtcgggggctccacccgcatccccaaggtgcagaagctgctgcaggacttcttcaacgggcgcgacctgaacaagagcatcaaccccgacgaggctgtgggctacggggcggcggtgcaggcggccatcctgatgggggacaagtccgagaacgtgcaggacctgctgctgctggacgtggctcccctgtcgctggggctggagacggccggaggcgtgatgactgccctgatcaagcgcaactccaccatccccaccaagcagacgcagatcttcaccacctactccgacaaccaacccggggtgctgatccaggtgtacgagggcgagagggccatgacgaaagacaacaatctgttggggcgcttcgagctgagcggcatccctccggccccaggcgtgccccagatcgaggtgaccttcgacatcgatgccaacggcatcctgaacgtcacggccacggacaagagcaccggcaaggccaacaagatcaccatcaccaacgacaagggccgcctgagcaaggaggagatcgagcgcatggtgcaggaggcggagaagtacaaagcggaggacgaggtgcagcgcgagagggtgtcagccaagaacgccctggagtcctacgccttcaacatgaagagcgccgtggaggatgaggggctcaagggcaagatcagcgaggccgacaagaagaaggtgctggacaagtgtcaagaggtcatctcgtggctggacgccaacaccttggccgagaaggacgagtttgagcacaagaggaaggagctggagcaggtgtgtaaccccatcatcagcggactgtaccagggtgccggtggtcccgggcctgggggcttcggggctcagggtcccaagggagggtctgggtcaggccccaccattgaggaggtagat SEQUENCE_PRIMER=acctgggcaccacctactc PRIMER_PRODUCT_SIZE_RANGE=1890-1920 = SEQUENCE_ID=tm_out_of_range_on_input PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MAX_TM=59.97 PRIMER_OPT_TM=59.97 SEQUENCE_TEMPLATE=atggccaaagccgcggcagtcggcatcgacctgggcaccacctactcctgcgtgggggtgttccaacacggcaaggtggagatcatcgccaacgaccagggcaaccgcaccacccccagctacgtggccttcacggacaccgagcggctcatcggggatgcggccaagaaccaggtggcgctgaacccgcagaacaccgtgtttgacgcgaagcgcctgatcggccgcaagttcggcgacccggtggtgcagtcggacatgaagcactggcctttccaggtgatcaacgacggagacaagcccaaggtgcaggtgagctacaagggggagaccaaggcattctaccccgaggagatctcgtccatggtgctgaccaagatgaaggagatcgccgaggcgtacctgggctacccggtgaccaacgcggtgatcaccgtgccggcctacttcaacgactcgcagcgccaggccaccaaggatgcgggtgtgatcgcggggctcaacgtgctgcggatcatcaacgagcccacggccgccgccatcgcctacggcctggacagaacgggcaagggggagcgcaacgtcctgatctttgacctgggcgggggcaccttcgacgtgtccatcctgacgatcgacgacggcatcttcgaggtgaaggccacggccggggacacccacctgggtggggaggactttgacaacaggctggtgaaccacttcgtggaggagttcaagagaaaacacaagaaggacatcagccagaacaagcgagccgtgaggcggctgcgcaccgcctgcgagagggccaagaggaccctgtcgtccagcacccaggccagcctggagatcgactccctgtttgagggcatcgacttctacacgtccatcaccagggcgaggttcgaggagctgtgctccgacctgttccgaagcaccctggagcccgtggagaaggctctgcgcgacgccaagctggacaaggcccagattcacgacctggtcctggtcgggggctccacccgcatccccaaggtgcagaagctgctgcaggacttcttcaacgggcgcgacctgaacaagagcatcaaccccgacgaggctgtgggctacggggcggcggtgcaggcggccatcctgatgggggacaagtccgagaacgtgcaggacctgctgctgctggacgtggctcccctgtcgctggggctggagacggccggaggcgtgatgactgccctgatcaagcgcaactccaccatccccaccaagcagacgcagatcttcaccacctactccgacaaccaacccggggtgctgatccaggtgtacgagggcgagagggccatgacgaaagacaacaatctgttggggcgcttcgagctgagcggcatccctccggccccaggcgtgccccagatcgaggtgaccttcgacatcgatgccaacggcatcctgaacgtcacggccacggacaagagcaccggcaaggccaacaagatcaccatcaccaacgacaagggccgcctgagcaaggaggagatcgagcgcatggtgcaggaggcggagaagtacaaagcggaggacgaggtgcagcgcgagagggtgtcagccaagaacgccctggagtcctacgccttcaacatgaagagcgccgtggaggatgaggggctcaagggcaagatcagcgaggccgacaagaagaaggtgctggacaagtgtcaagaggtcatctcgtggctggacgccaacaccttggccgagaaggacgagtttgagcacaagaggaaggagctggagcaggtgtgtaaccccatcatcagcggactgtaccagggtgccggtggtcccgggcctgggggcttcggggctcagggtcccaagggagggtctgggtcaggccccaccattgaggaggtagat SEQUENCE_PRIMER=acctgggcaccacctactc PRIMER_PRODUCT_SIZE_RANGE=1890-1920 = primer3-2.3.6/test/primer_start_codon_output0000664000077300007730000100410412231156245020275 0ustar ioanaioanaPRIMER_PRODUCT_SIZE_RANGE=75-20000 PRIMER_MAX_END_STABILITY=10 PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_SIZE=19 PRIMER_MAX_TM=75 PRIMER_NUM_RETURN=1 SEQUENCE_ID=test0 SEQUENCE_TEMPLATE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA SEQUENCE_START_CODON_POSITION=-3 PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 14, ok 4 PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=75 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=69.797698 PRIMER_LEFT_0_PENALTY=68.113745 PRIMER_RIGHT_0_PENALTY=1.683953 PRIMER_LEFT_0_SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAA PRIMER_RIGHT_0_SEQUENCE=ACTGAGCAAGGAAGCTCCG PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=75,19 PRIMER_LEFT_0_TM=58.886 PRIMER_RIGHT_0_TM=60.684 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_POSITION_PENALTY=60.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=9.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 = SEQUENCE_ID=test1 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA SEQUENCE_START_CODON_POSITION=0 PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4 PRIMER_RIGHT_EXPLAIN=considered 33, low tm 2, high 3' stability 1, ok 30 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=75 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=8.205547 PRIMER_LEFT_0_PENALTY=7.037233 PRIMER_RIGHT_0_PENALTY=1.168315 PRIMER_LEFT_0_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG PRIMER_RIGHT_0_SEQUENCE=TACTGAGCAAGGAAGCTCCG PRIMER_LEFT_0=0,25 PRIMER_RIGHT_0=76,20 PRIMER_LEFT_0_TM=57.963 PRIMER_RIGHT_0_TM=60.668 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.500000 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=9.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=77 = SEQUENCE_ID=test2 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA SEQUENCE_START_CODON_POSITION=0 PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4 PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=75 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=8.721185 PRIMER_LEFT_0_PENALTY=7.037233 PRIMER_RIGHT_0_PENALTY=1.683953 PRIMER_LEFT_0_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG PRIMER_RIGHT_0_SEQUENCE=ACTGAGCAAGGAAGCTCCG PRIMER_LEFT_0=0,25 PRIMER_RIGHT_0=75,19 PRIMER_LEFT_0_TM=57.963 PRIMER_RIGHT_0_TM=60.684 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=9.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 = SEQUENCE_ID=test3 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA SEQUENCE_START_CODON_POSITION=0 PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4 PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=75 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=8.721185 PRIMER_LEFT_0_PENALTY=7.037233 PRIMER_RIGHT_0_PENALTY=1.683953 PRIMER_LEFT_0_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG PRIMER_RIGHT_0_SEQUENCE=ACTGAGCAAGGAAGCTCCG PRIMER_LEFT_0=0,25 PRIMER_RIGHT_0=75,19 PRIMER_LEFT_0_TM=57.963 PRIMER_RIGHT_0_TM=60.684 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=9.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=76 = SEQUENCE_ID=test4 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA SEQUENCE_START_CODON_POSITION=0 PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4 PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=-1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=9.930660 PRIMER_LEFT_0_PENALTY=7.037233 PRIMER_RIGHT_0_PENALTY=2.893427 PRIMER_LEFT_0_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG PRIMER_RIGHT_0_SEQUENCE=TATCTGAGCAAGGAAGCTCCG PRIMER_LEFT_0=0,25 PRIMER_RIGHT_0=77,21 PRIMER_LEFT_0_TM=57.963 PRIMER_RIGHT_0_TM=61.893 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=9.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=78 = SEQUENCE_ID=test5 SEQUENCE_TEMPLATE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA SEQUENCE_START_CODON_POSITION=0 PRIMER_ERROR=No start codon at SEQUENCE_START_CODON_POSITION = SEQUENCE_ID=test6 SEQUENCE_INCLUDED_REGION=0,299 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAATGATACTTCATTCCAGA SEQUENCE_START_CODON_POSITION=298 P3_COMMENT=Should fail with "Start codon not contained in INCLUDED_REGION". PRIMER_ERROR=Start codon position not contained in SEQUENCE_INCLUDED_REGION = SEQUENCE_ID=test7 SEQUENCE_INCLUDED_REGION=0,300 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATAATGATGATACTTCATTCCATG SEQUENCE_START_CODON_POSITION=297 P3_COMMENT=Should succeed. PRIMER_LEFT_EXPLAIN=considered 1864, would not amplify any of the ORF 1229, low tm 87, high tm 53, high 3' stability 25, ok 470 PRIMER_RIGHT_EXPLAIN=considered 1840, low tm 186, high tm 161, high 3' stability 25, ok 1468 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=-1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=2163.830281 PRIMER_LEFT_0_PENALTY=2160.043439 PRIMER_RIGHT_0_PENALTY=3.786842 PRIMER_LEFT_0_SEQUENCE=AAAGATGGCATTCATCTGGC PRIMER_RIGHT_0_SEQUENCE=CATTATCAGCAGAGACCCATTT PRIMER_LEFT_0=225,20 PRIMER_RIGHT_0=299,22 PRIMER_LEFT_0_TM=60.043 PRIMER_RIGHT_0_TM=58.213 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_POSITION_PENALTY=2160.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 = SEQUENCE_ID=test8 PRIMER_INSIDE_PENALTY=100.0 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA SEQUENCE_START_CODON_POSITION=0 PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0 PRIMER_ERROR=Cannot accept both SEQUENCE_START_CODON_POSITION and non-default arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY = SEQUENCE_ID=test9 PRIMER_INSIDE_PENALTY=-1.0 PRIMER_OUTSIDE_PENALTY=100.0 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA SEQUENCE_START_CODON_POSITION=0 PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0 PRIMER_ERROR=Cannot accept both SEQUENCE_START_CODON_POSITION and non-default arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY = PRIMER_PRODUCT_SIZE_RANGE=75-20000 PRIMER_OUTSIDE_PENALTY=0.0 PRIMER_MAX_END_STABILITY=10 PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_SIZE=19 PRIMER_MAX_TM=65 PRIMER_NUM_RETURN=20 SEQUENCE_ID=DCC/X76132 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGCGCGCATCTTCAAGTAACCGGTTTTCAAATTAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAAACATACTTCATTCCAGACACCACAAGCCAGATGAGGGACTTTACCAATGTGAGGCATCTTTAGGAGATTCTGGCTCAATTATTAGTCGGACAGCAAAAGTTGCAGTAGCAGGACCACTGAGGTTCCTTTCACAGACAGAATCTGTCACAGCCTTCATGGGAGACACAGTGCTACTCAAGTGTGAAGTCATTGGGGAGCCCATGCCAACAATCCACTGGCAGAAGAACCAACAAGACCTGACTCCAATCCCAGGTGACTCCCGAGTGGTGGTCTTGCCCTCTGGAGCATTGCAGATCAGCCGACTCCAACCGGGGGACATTGGAATTTACCGATGCTCAGCTCGAAATCCAGCCAGCTCAAGAACAGGAAATGAAGCAGAAGTCAGAATTTTATCAGATCCAGGACTGCATAGACAGCTGTATTTTCTGCAAAGACCATCCAATGTAGTAGCCATTGAAGGAAAAGATGCTGTCCTGGAATGTTGTGTTTCTGGCTATCCTCCACCAAGTTTTACCTGGTTACGAGGCGAGGAAGTCATCCAACTCAGGTCTAAAAAGTATTCTTTATTGGGTGGAAGCAACTTGCTTATCTCCAATGTGACAGATGATGACAGTGGAATGTATACCTGTGTTGTCACATATAAAAATGAGAATATTAGTGCCTCTGCAGAGCTCACAGTCTTGGTTCCGCCATGGTTTTTAAATCATCCTTCCAACCTGTATGCCTATGAAAGCATGGATATTGAGTTTGAATGTACAGTCTCTGGAAAGCCTGTGCCCACTGTGAATTGGATGAAGAATGGAGATGTGGTCATTCCTAGTGATTATTTTCAGATAGTGGGAGGAAGCAACTTACGGATACTTGGGGTGGTGAAGTCAGATGAAGGCTTTTATCAATGTGTGGCTGAAAATGAGGCTGGAAATGCCCAGACCAGTGCACAGCTCATTGTCCCTAAGCCTGCAATCCCAAGCTCCAGTGTCCTCCCTTCGGCTCCCAGAGATGTGGTCCCTGTCTTGGTTTCCAGCCGATTTGTCCGTCTCAGCTGGCGCCCACCTGCAGAAGCGAAAGGGAACATTCAAACTTTCACGGTCTTTTTCTCCAGAGAAGGTGACAACAGGGAACGAGCATTGAATACAACACAGCCTGGGTCCCTTCAGCTCACTGTGGGAAACCTGAAGCCAGAAGCCATGTACACCTTTCGAGTTGTGGCTTACAATGAATGGGGACCGGGAGAGAGTTCTCAACCCATCAAGGTGGCCACACAGCCTGAGTTGCAAGTTCCAGGGCCAGTAGAAAACCTGCAAGCTGTATCTACCTCACCTACCTCAATTCTTATTACCTGGGAACCCCCTGCCTATGCAAACGGTCCAGTCCAAGGTTACAGATTGTTCTGCACTGAGGTGTCCACAGGAAAAGAACAGAATATAGAGGTTGATGGACTATCTTATAAACTGGAAGGCCTGAAAAAATTCACCGAATATAGTCTTCGATTCTTAGCTTATAATCGCTATGGTCCGGGCGTCTCTACTGATGATATAACAGTGGTTACACTTTCTGACGTGCCAAGTGCCCCGCCTCAGAACGTCTCCCTGGAAGTGGTCAATTCAAGAAGTATCAAAGTTAGCTGGCTGCCTCCTCCATCAGGAACACAAAATGGATTTATTACCGGCTATAAAATTCGACACAGAAAGACGACCCGCAGGGGTGAGATGGAAACACTGGAGCCAAACAACCTCTGGTACCTATTCACAGGACTGGAGAAAGGAAGTCAGTACAGTTTCCAGGTGTCAGCCATGACAGTCAATGGTACTGGACCACCTTCCAACTGGTATACTGCAGAGACTCCAGAGAATGATCTAGATGAATCTCAAGTTCCTGATCAACCAAGCTCTCTTCATGTGAGGCCCCAGACTAACTGCATCATCATGAGTTGGACTCCTCCCTTGAACCCAAACATCGTGGTGCGAGGTTATATTATCGGTTATGGCGTTGGGAGCCCTTACGCTGAGACAGTGCGTGTGGACAGCAAGCAGCGATATTATTCCATTGAGAGGTTAGAGTCAAGTTCCCATTATGTAATCTCCCTAAAAGCTTTTAACAATGCCGGAGAAGGAGTTCCTCTTTATGAAAGTGCCACCACCAGGTCTATAACCGATCCCACTGACCCAGTTGATTATTATCCTTTGCTTGATGATTTCCCCACCTCGGTCCCAGATCTCTCCACCCCCATGCTCCCACCAGTAGGTGTACAGGCTGTGGCTCTTACCCATGATGCTGTGAGGGTCAGCTGGGCAGACAACTCTGTCCCTAAGAACCAAAAGACGTCTGAGGTGCGACTTTACACCGTCCGGTGGAGAACCAGCTTTTCTGCAAGTGCAAAATACAAGTCAGAAGACACAACATCTCTAAGTTACACAGCAACAGGCCTCAAACCAAACACAATGTATGAATTCTCGGTCATGGTAACAAAAAACAGAAGGTCCAGTACTTGGAGCATGACTGCACATGCCACCACGTATGAAGCAGCCCCCACCTCTGCTCCCAAGGACTTTACAGTCATTACTAGGGAAGGGAAGCCTCGTGCCGTCATTGTGAGTTGGCAGCCTCCCTTGGAAGCCAATGGGAAAATTACTGCTTACATCTTATTTTATACCTTGGACAAGAACATCCCAATTGATGACTGGATTATGGAAACAATCAGTGGTGATAGGCTTACTCATCAAATCATGGATCTCAACCTTGATACTATGTATTACTTTCGAATTCAAGCACGAAATTCAAAAGGAGTGGGGCCACTCTCTGATCCCATCCTCTTCAGGACTCTGAAAGTGGAACACCCTGACAAAATGGCTAATGACCAAGGTCGTCATGGAGATGGAGGTTATTGGCCAGTTGATACTAATTTGATTGATAGAAGCACCCTAAATGAGCCGCCAATTGGACAAATGCACCCCCCGCATGGCAGTGTCACTCCTCAGAAGAACAGCAACCTGCTTGTGATCATTGTGGTCACCGTTGGTGTCATCACAGTGCTGGTAGTGGTCATCGTGGCTGTGATTTGCACCCGACGCTCTTCAGCCCAGCAGAGAAAGAAACGGGCCACCCACAGTGCTGGCAAAAGGAAGGGCAGCCAGAAGGACCTCCGACCCCCTGATCTTTGGATCCATCATGAAGAAATGGAGATGAAAAATATTGAAAAGCCATCTGGCACTGACCCTGCAGGAAGGGACTCTCCCATCCAAAGTTGCCAAGACCTCACACCAGTCAGCCACAGCCAGTCAGAAACCCAACTGGGAAGCAAAAGCACCTCTCATTCAGGTCAAGACACTGAGGAAGCAGGGAGCTCTATGTCCACTCTGGAGAGGTCGCTGGCTGCACGCCGAGCCCCCCGGGCCAAGCTCATGATTCCCATGGATGCCCAGTCCAACAATCCTGCTGTCGTGAGCGCCATCCCGGTGCCAACGCTAGAAAGTGCCCAGTACCCAGGAATCCTCCCGTCTCCCACCTGTGGATATCCCCACCCGCAGTTCACTCTCCGGCCTGTGCCATTCCCAACACTCTCAGTGGACCGAGGTTTCGGAGCAGGAAGAAGTCAGTCAGTGAGTGAAGGACCAACTACCCAACAACCACCTATGCTGCCCCCATCTCAGCCTGAGCATTCTAGCAGCGAGGAGGCACCAAGCAGAACCATCCCCACAGCTTGTGTTCGACCAACTCACCCACTCCGCAGCTTTGCTAATCCTTTGCTACCTCCACCAATGAGTGCAATAGAACCGAAAGTCCCTTACACACCACTTTTGTCTCAGCCAGGGCCCACTCTTCCTAAGACCCATGTGAAAACAGCCTCCCTTGGGTTGGCTGGAAAAGCAAGATCCCCTTTGCTTCCTGTGTCTGTGCCAACAGCCCCTGAAGTGTCTGAGGAGAGCCACAAACCAACAGAGGATTCAGCCAATGTGTATGAACAGGATGATCTGAGTGAACAAATGGCAAGTTTGGAAGGACTCATGAAGCAGCTTAATGCCATCACAGGCTCAGCCTTTTAACATGTATTTCTGAATGGATGAGGTGAATTTTCCGGGAACTTTGCAGCATACCAATTACCCATAAACAGCACACCTGTGTCCAAGAACTCTAACCAGTGTACAGGTCACCCATCAGGACCACTCAGTTAAGGAAGATCCTGAAGCAGTTCAGAAGGAATAAGCATTCCTTCTTTCACAGGCATCAGGAATTGTCAAATGATGATTATGAGTTCCCTAAACAAAAGCAAAGATGCATTTTCACTGCAATGTCAAAGTTTAGCTGCT SEQUENCE_START_CODON_POSITION=0 PRIMER_LEFT_EXPLAIN=considered 39774, would not amplify any of the ORF 27031, GC content failed 49, low tm 2568, high tm 5293, long poly-x seq 73, high 3' stability 94, ok 4666 PRIMER_RIGHT_EXPLAIN=considered 39257, GC content failed 148, low tm 8235, high tm 15926, high any compl 53, high end compl 11, long poly-x seq 76, high 3' stability 140, ok 14668 PRIMER_PAIR_EXPLAIN=considered 22, ok 22 PRIMER_STOP_CODON_POSITION=4341 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=20.422177 PRIMER_LEFT_0_PENALTY=7.184184 PRIMER_RIGHT_0_PENALTY=13.237993 PRIMER_LEFT_0_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_0_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=4356,25 PRIMER_LEFT_0_TM=60.184 PRIMER_RIGHT_0_TM=59.262 PRIMER_LEFT_0_GC_PERCENT=37.037 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=7.500000 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=4357 PRIMER_PAIR_1_PENALTY=20.559156 PRIMER_LEFT_1_PENALTY=7.184184 PRIMER_RIGHT_1_PENALTY=13.374972 PRIMER_LEFT_1_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_1_SEQUENCE=CAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_1=0,27 PRIMER_RIGHT_1=4355,24 PRIMER_LEFT_1_TM=60.184 PRIMER_RIGHT_1_TM=57.625 PRIMER_LEFT_1_GC_PERCENT=37.037 PRIMER_RIGHT_1_GC_PERCENT=33.333 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=7.000000 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=4356 PRIMER_PAIR_2_PENALTY=20.657955 PRIMER_LEFT_2_PENALTY=7.184184 PRIMER_RIGHT_2_PENALTY=13.473771 PRIMER_LEFT_2_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_2_SEQUENCE=CAGAAATACATGTTAAAAGGCTGAG PRIMER_LEFT_2=0,27 PRIMER_RIGHT_2=4355,25 PRIMER_LEFT_2_TM=60.184 PRIMER_RIGHT_2_TM=58.526 PRIMER_LEFT_2_GC_PERCENT=37.037 PRIMER_RIGHT_2_GC_PERCENT=36.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=7.000000 PRIMER_LEFT_2_END_STABILITY=8.8000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=4356 PRIMER_PAIR_3_PENALTY=20.761130 PRIMER_LEFT_3_PENALTY=7.184184 PRIMER_RIGHT_3_PENALTY=13.576946 PRIMER_LEFT_3_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_3_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGAG PRIMER_LEFT_3=0,27 PRIMER_RIGHT_3=4356,26 PRIMER_LEFT_3_TM=60.184 PRIMER_RIGHT_3_TM=60.077 PRIMER_LEFT_3_GC_PERCENT=37.037 PRIMER_RIGHT_3_GC_PERCENT=34.615 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=7.500000 PRIMER_LEFT_3_END_STABILITY=8.8000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=4357 PRIMER_PAIR_4_PENALTY=21.059156 PRIMER_LEFT_4_PENALTY=7.184184 PRIMER_RIGHT_4_PENALTY=13.874972 PRIMER_LEFT_4_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_4_SEQUENCE=TCAGAAATACATGTTAAAAGGCTG PRIMER_LEFT_4=0,27 PRIMER_RIGHT_4=4356,24 PRIMER_LEFT_4_TM=60.184 PRIMER_RIGHT_4_TM=57.625 PRIMER_LEFT_4_GC_PERCENT=37.037 PRIMER_RIGHT_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=7.500000 PRIMER_LEFT_4_END_STABILITY=8.8000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=4357 PRIMER_PAIR_5_PENALTY=21.335332 PRIMER_LEFT_5_PENALTY=7.184184 PRIMER_RIGHT_5_PENALTY=14.151147 PRIMER_LEFT_5_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_5_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTG PRIMER_LEFT_5=0,27 PRIMER_RIGHT_5=4357,25 PRIMER_LEFT_5_TM=60.184 PRIMER_RIGHT_5_TM=58.849 PRIMER_LEFT_5_GC_PERCENT=37.037 PRIMER_RIGHT_5_GC_PERCENT=32.000 PRIMER_LEFT_5_SELF_ANY=6.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=8.000000 PRIMER_LEFT_5_END_STABILITY=8.8000 PRIMER_RIGHT_5_END_STABILITY=9.7000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=4358 PRIMER_PAIR_6_PENALTY=21.566466 PRIMER_LEFT_6_PENALTY=7.184184 PRIMER_RIGHT_6_PENALTY=14.382282 PRIMER_LEFT_6_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_6_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_6=0,27 PRIMER_RIGHT_6=4357,26 PRIMER_LEFT_6_TM=60.184 PRIMER_RIGHT_6_TM=60.382 PRIMER_LEFT_6_GC_PERCENT=37.037 PRIMER_RIGHT_6_GC_PERCENT=30.769 PRIMER_LEFT_6_SELF_ANY=6.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=8.000000 PRIMER_LEFT_6_END_STABILITY=8.8000 PRIMER_RIGHT_6_END_STABILITY=8.2000 PRIMER_PAIR_6_COMPL_ANY=6.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PAIR_6_PRODUCT_SIZE=4358 PRIMER_PAIR_7_PENALTY=22.076208 PRIMER_LEFT_7_PENALTY=8.838215 PRIMER_RIGHT_7_PENALTY=13.237993 PRIMER_LEFT_7_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_7_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_7=0,26 PRIMER_RIGHT_7=4356,25 PRIMER_LEFT_7_TM=57.162 PRIMER_RIGHT_7_TM=59.262 PRIMER_LEFT_7_GC_PERCENT=34.615 PRIMER_RIGHT_7_GC_PERCENT=32.000 PRIMER_LEFT_7_SELF_ANY=6.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=7.500000 PRIMER_LEFT_7_END_STABILITY=7.0000 PRIMER_RIGHT_7_END_STABILITY=8.2000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PAIR_7_PRODUCT_SIZE=4357 PRIMER_PAIR_8_PENALTY=22.213187 PRIMER_LEFT_8_PENALTY=8.838215 PRIMER_RIGHT_8_PENALTY=13.374972 PRIMER_LEFT_8_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_8_SEQUENCE=CAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_8=0,26 PRIMER_RIGHT_8=4355,24 PRIMER_LEFT_8_TM=57.162 PRIMER_RIGHT_8_TM=57.625 PRIMER_LEFT_8_GC_PERCENT=34.615 PRIMER_RIGHT_8_GC_PERCENT=33.333 PRIMER_LEFT_8_SELF_ANY=6.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=1.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=7.000000 PRIMER_LEFT_8_END_STABILITY=7.0000 PRIMER_RIGHT_8_END_STABILITY=8.2000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PAIR_8_PRODUCT_SIZE=4356 PRIMER_PAIR_9_PENALTY=22.311986 PRIMER_LEFT_9_PENALTY=8.838215 PRIMER_RIGHT_9_PENALTY=13.473771 PRIMER_LEFT_9_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_9_SEQUENCE=CAGAAATACATGTTAAAAGGCTGAG PRIMER_LEFT_9=0,26 PRIMER_RIGHT_9=4355,25 PRIMER_LEFT_9_TM=57.162 PRIMER_RIGHT_9_TM=58.526 PRIMER_LEFT_9_GC_PERCENT=34.615 PRIMER_RIGHT_9_GC_PERCENT=36.000 PRIMER_LEFT_9_SELF_ANY=6.00 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=7.000000 PRIMER_LEFT_9_END_STABILITY=7.0000 PRIMER_RIGHT_9_END_STABILITY=6.7000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=4356 PRIMER_PAIR_10_PENALTY=22.415161 PRIMER_LEFT_10_PENALTY=8.838215 PRIMER_RIGHT_10_PENALTY=13.576946 PRIMER_LEFT_10_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_10_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGAG PRIMER_LEFT_10=0,26 PRIMER_RIGHT_10=4356,26 PRIMER_LEFT_10_TM=57.162 PRIMER_RIGHT_10_TM=60.077 PRIMER_LEFT_10_GC_PERCENT=34.615 PRIMER_RIGHT_10_GC_PERCENT=34.615 PRIMER_LEFT_10_SELF_ANY=6.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=3.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=7.500000 PRIMER_LEFT_10_END_STABILITY=7.0000 PRIMER_RIGHT_10_END_STABILITY=6.7000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=4357 PRIMER_PAIR_11_PENALTY=22.564003 PRIMER_LEFT_11_PENALTY=7.184184 PRIMER_RIGHT_11_PENALTY=15.379819 PRIMER_LEFT_11_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_11_SEQUENCE=ATTCAGAAATACATGTTAAAAGGCTG PRIMER_LEFT_11=0,27 PRIMER_RIGHT_11=4358,26 PRIMER_LEFT_11_TM=60.184 PRIMER_RIGHT_11_TM=59.120 PRIMER_LEFT_11_GC_PERCENT=37.037 PRIMER_RIGHT_11_GC_PERCENT=30.769 PRIMER_LEFT_11_SELF_ANY=6.00 PRIMER_RIGHT_11_SELF_ANY=6.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=1.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=8.500000 PRIMER_LEFT_11_END_STABILITY=8.8000 PRIMER_RIGHT_11_END_STABILITY=9.7000 PRIMER_PAIR_11_COMPL_ANY=6.00 PRIMER_PAIR_11_COMPL_END=1.00 PRIMER_PAIR_11_PRODUCT_SIZE=4359 PRIMER_PAIR_12_PENALTY=22.713187 PRIMER_LEFT_12_PENALTY=8.838215 PRIMER_RIGHT_12_PENALTY=13.874972 PRIMER_LEFT_12_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_12_SEQUENCE=TCAGAAATACATGTTAAAAGGCTG PRIMER_LEFT_12=0,26 PRIMER_RIGHT_12=4356,24 PRIMER_LEFT_12_TM=57.162 PRIMER_RIGHT_12_TM=57.625 PRIMER_LEFT_12_GC_PERCENT=34.615 PRIMER_RIGHT_12_GC_PERCENT=33.333 PRIMER_LEFT_12_SELF_ANY=6.00 PRIMER_RIGHT_12_SELF_ANY=6.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=1.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=7.500000 PRIMER_LEFT_12_END_STABILITY=7.0000 PRIMER_RIGHT_12_END_STABILITY=9.7000 PRIMER_PAIR_12_COMPL_ANY=6.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PAIR_12_PRODUCT_SIZE=4357 PRIMER_PAIR_13_PENALTY=22.895624 PRIMER_LEFT_13_PENALTY=7.184184 PRIMER_RIGHT_13_PENALTY=15.711440 PRIMER_LEFT_13_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_13_SEQUENCE=CATTCAGAAATACATGTTAAAAGGC PRIMER_LEFT_13=0,27 PRIMER_RIGHT_13=4359,25 PRIMER_LEFT_13_TM=60.184 PRIMER_RIGHT_13_TM=58.289 PRIMER_LEFT_13_GC_PERCENT=37.037 PRIMER_RIGHT_13_GC_PERCENT=32.000 PRIMER_LEFT_13_SELF_ANY=6.00 PRIMER_RIGHT_13_SELF_ANY=6.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=9.000000 PRIMER_LEFT_13_END_STABILITY=8.8000 PRIMER_RIGHT_13_END_STABILITY=9.7000 PRIMER_PAIR_13_COMPL_ANY=6.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PAIR_13_PRODUCT_SIZE=4360 PRIMER_PAIR_14_PENALTY=22.989362 PRIMER_LEFT_14_PENALTY=8.838215 PRIMER_RIGHT_14_PENALTY=14.151147 PRIMER_LEFT_14_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_14_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTG PRIMER_LEFT_14=0,26 PRIMER_RIGHT_14=4357,25 PRIMER_LEFT_14_TM=57.162 PRIMER_RIGHT_14_TM=58.849 PRIMER_LEFT_14_GC_PERCENT=34.615 PRIMER_RIGHT_14_GC_PERCENT=32.000 PRIMER_LEFT_14_SELF_ANY=6.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=8.000000 PRIMER_LEFT_14_END_STABILITY=7.0000 PRIMER_RIGHT_14_END_STABILITY=9.7000 PRIMER_PAIR_14_COMPL_ANY=6.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PAIR_14_PRODUCT_SIZE=4358 PRIMER_PAIR_15_PENALTY=23.064003 PRIMER_LEFT_15_PENALTY=7.184184 PRIMER_RIGHT_15_PENALTY=15.879819 PRIMER_LEFT_15_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_15_SEQUENCE=CATTCAGAAATACATGTTAAAAGGCT PRIMER_LEFT_15=0,27 PRIMER_RIGHT_15=4359,26 PRIMER_LEFT_15_TM=60.184 PRIMER_RIGHT_15_TM=59.120 PRIMER_LEFT_15_GC_PERCENT=37.037 PRIMER_RIGHT_15_GC_PERCENT=30.769 PRIMER_LEFT_15_SELF_ANY=6.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=3.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=9.000000 PRIMER_LEFT_15_END_STABILITY=8.8000 PRIMER_RIGHT_15_END_STABILITY=9.4000 PRIMER_PAIR_15_COMPL_ANY=6.00 PRIMER_PAIR_15_COMPL_END=2.00 PRIMER_PAIR_15_PRODUCT_SIZE=4360 PRIMER_PAIR_16_PENALTY=23.220496 PRIMER_LEFT_16_PENALTY=8.838215 PRIMER_RIGHT_16_PENALTY=14.382282 PRIMER_LEFT_16_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_16_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_16=0,26 PRIMER_RIGHT_16=4357,26 PRIMER_LEFT_16_TM=57.162 PRIMER_RIGHT_16_TM=60.382 PRIMER_LEFT_16_GC_PERCENT=34.615 PRIMER_RIGHT_16_GC_PERCENT=30.769 PRIMER_LEFT_16_SELF_ANY=6.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=1.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=8.000000 PRIMER_LEFT_16_END_STABILITY=7.0000 PRIMER_RIGHT_16_END_STABILITY=8.2000 PRIMER_PAIR_16_COMPL_ANY=6.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PAIR_16_PRODUCT_SIZE=4358 PRIMER_PAIR_17_PENALTY=23.266175 PRIMER_LEFT_17_PENALTY=7.184184 PRIMER_RIGHT_17_PENALTY=16.081991 PRIMER_LEFT_17_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_17_SEQUENCE=ATTCAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_17=0,27 PRIMER_RIGHT_17=4358,27 PRIMER_LEFT_17_TM=60.184 PRIMER_RIGHT_17_TM=60.582 PRIMER_LEFT_17_GC_PERCENT=37.037 PRIMER_RIGHT_17_GC_PERCENT=29.630 PRIMER_LEFT_17_SELF_ANY=6.00 PRIMER_RIGHT_17_SELF_ANY=6.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=1.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=8.500000 PRIMER_LEFT_17_END_STABILITY=8.8000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=6.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PAIR_17_PRODUCT_SIZE=4359 PRIMER_PAIR_18_PENALTY=23.304836 PRIMER_LEFT_18_PENALTY=7.184184 PRIMER_RIGHT_18_PENALTY=16.120652 PRIMER_LEFT_18_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_18_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGAG PRIMER_LEFT_18=0,27 PRIMER_RIGHT_18=4357,27 PRIMER_LEFT_18_TM=60.184 PRIMER_RIGHT_18_TM=61.121 PRIMER_LEFT_18_GC_PERCENT=37.037 PRIMER_RIGHT_18_GC_PERCENT=33.333 PRIMER_LEFT_18_SELF_ANY=6.00 PRIMER_RIGHT_18_SELF_ANY=6.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=3.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=8.000000 PRIMER_LEFT_18_END_STABILITY=8.8000 PRIMER_RIGHT_18_END_STABILITY=6.7000 PRIMER_PAIR_18_COMPL_ANY=6.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PAIR_18_PRODUCT_SIZE=4358 PRIMER_PAIR_19_PENALTY=23.871468 PRIMER_LEFT_19_PENALTY=7.184184 PRIMER_RIGHT_19_PENALTY=16.687284 PRIMER_LEFT_19_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_19_SEQUENCE=CCATTCAGAAATACATGTTAAAAGGC PRIMER_LEFT_19=0,27 PRIMER_RIGHT_19=4360,26 PRIMER_LEFT_19_TM=60.184 PRIMER_RIGHT_19_TM=61.187 PRIMER_LEFT_19_GC_PERCENT=37.037 PRIMER_RIGHT_19_GC_PERCENT=34.615 PRIMER_LEFT_19_SELF_ANY=6.00 PRIMER_RIGHT_19_SELF_ANY=6.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=9.500000 PRIMER_LEFT_19_END_STABILITY=8.8000 PRIMER_RIGHT_19_END_STABILITY=9.7000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=1.00 PRIMER_PAIR_19_PRODUCT_SIZE=4361 = SEQUENCE_ID=LEU8/L-SELECTING/X17519 SEQUENCE_TEMPLATE=CTCCCTTTGGGCAAGGACCTGAGACCCTTGTGCTAAGTCAAGAGGCTCAATGGGCTGCAGAAGAACTAGAGAAGGACCAAGCAAAGCCATGATATTTCCATGGAAATGTCAGAGCACCCAGAGGGACTTATGGAACATCTTCAAGTTGTGGGGGTGGACAATGCTCTGTTGTGATTTCCTGGCACATCATGGAACCGACTGCTGGACTTACCATTATTCTGAAAAACCCATGAACTGGCAAAGGGCTAGAAGATTCTGCCGAGACAATTACACAGATTTAGTTGCCATACAAAACAAGGCGGAAATTGAGTATCTGGAGAAGACTCTGCCTTTCAGTCGTTCTTACTACTGGATAGGAATCCGGAAGATAGGAGGAATATGGACGTGGGTGGGAACCAACAAATCTCTCACTGAAGAAGCAGAGAACTGGGGAGATGGTGAGCCCAACAACAAGAAGAACAAGGAGGACTGCGTGGAGATCTATATCAAGAGAAACAAAGATGCAGGCAAATGGAACGATGACGCCTGCCACAAACTAAAGGCAGCCCTCTGTTACACAGCTTCTTGCCAGCCCTGGTCATGCAGTGGCCATGGAGAATGTGTAGAAATCATCAATAATTACACCTGCAACTGTGATGTGGGGTACTATGGGCCCCAGTGTCAGTTTGTGATTCAGTGTGAGCCTTTGGAGGCCCCAGAGCTGGGTACCATGGACTGTACTCACTCTTTGGGAAACTTCAGCTTCAGCTCACAGTGTGCCTTCAGCTGCTCTGAAGGAACAAACTTAACTGGGATTGAAGAAACCACCTGTGGACCATTTGGAAACTGGTCATCTCCAGAACCAACCTGTCAAGTGATTCAGTGTGAGCCTCTATCAGCACCAGATTTGGGGATCATGAACTGTAGCCATCCCCTGGCCAGCTTCAGCTTTACCTCTGCATGTACCTTCATCTGCTCAGAAGGAACTGAGTTAATTGGGAAGAAGAAAACCATTTGTGAATCATCTGGAATCTGGTCAAATCCTAGTCCAATATGTCAAAAATTGGACAAAAGTTTCTCAATGATTAAGGAGGGTGATTATAACCCCCTCTTCATTCCAGTGGCAGTCATGGTTACTGCATTCTCTGGGTTGGCATTTATCATTTGGCTGGCAAGGAGATTAAAAAAAGGCAAGAAATCCAAGAGAAGTATGAATGACCCATATTAAATCGCCCTTGGTGAAAGAAAATTCTTGGAATACTAAAAATCATGAGATCCTTTAAATCCTTCCATGAAACGTTTTGTGTGGTGGCACCTCCTACGTCAAACATGAAGTGTGTTTCCTTCAGTGCATCTGGGAAGATTTCTACCTGACCAACAGTTCCTTCAGCTTCCATTTCGCCCCTCATTTATCCCTCAACCCCCAGCCCACAGGTGTTTATACAGCTCAGCTTTTTGTCTTTTCTGAGGAGAAACAAATAAGACCATAAAGGGAAAGGATTCATGTGGAATATAAAGATGGCTGACTTTGCTCTTTCTTGACTCTTGTTTTCAGTTTCAATTCAGTGCTGTACTTGATGACAGACACTTCTAAATGAAGTGCAAATTTGATACATATGTGAATATGGACTCAGTTTTCTTGCAGATCAAATTTCACGTCGTCTTCTGTATACTGTGGAGGTACACTCTTATAGAAAGTTCAAAAAGTCTACGCTCTCCTTTCTTTCTAACTCCAGTGAAGTAATGGGGTCCTGCTCAAGTTGAAAGAGTCCTATTTGCACTGTAGCCTCGCCGTCTGTGAATTGGACCATCCTATTTAACTGGCTTCAGCCTCCCCACCTTCTTCAGCCACCTCTCTTTTTCAGTTGGCTGACTTCCACACCTAGCATCTCATGAGTGCCAAGCAAAAGGAGAGAAGAGAGAAATAGCCTGCGCTGTTTTTTAGTTTGGGGGTTTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTTTCTTTTATCACGATATTATTAGTAAGAAAACATCACTGAAATGCTAGCTGCAAGTGACATCTCTTTGATGTCATATGGAAGAGTTAAAACAGGTGGAGAAATTCCTTGATTCACAATGAAATGCTCTCCTTTCCCCTGCCCCCAGACCTTTTATCCGACTTACCTAGATTCTACATATTCTTTAAATTTCATCTCAGGCCTCCCTCAACCCCACCACTTCTTTTATAACTAGTCCTTTACTAATCCAACCCATGATGAGCTCCTCTTCCTGGCTTCTTACTGAAAGGTTACCCTGTAACATGCAATTTTGCATTTGAATAAAGCCTGCTTTTTAAGTGTT SEQUENCE_START_CODON_POSITION=49 PRIMER_LEFT_EXPLAIN=considered 19864, would not amplify any of the ORF 16423, low tm 794, high tm 1127, high end compl 1, long poly-x seq 23, high 3' stability 37, ok 1459 PRIMER_RIGHT_EXPLAIN=considered 19472, GC content failed 127, low tm 6190, high tm 5041, high end compl 1, long poly-x seq 45, high 3' stability 67, ok 8001 PRIMER_PAIR_EXPLAIN=considered 24, high end compl 2, ok 22 PRIMER_STOP_CODON_POSITION=1204 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=6.788400 PRIMER_LEFT_0_PENALTY=1.909478 PRIMER_RIGHT_0_PENALTY=4.878922 PRIMER_LEFT_0_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_0_SEQUENCE=GGGCGATTTAATATGGGTCA PRIMER_LEFT_0=49,21 PRIMER_RIGHT_0=1213,20 PRIMER_LEFT_0_TM=59.091 PRIMER_RIGHT_0_TM=59.621 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=4.500000 PRIMER_LEFT_0_END_STABILITY=5.7000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=2.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=1165 PRIMER_PAIR_1_PENALTY=6.918802 PRIMER_LEFT_1_PENALTY=2.039879 PRIMER_RIGHT_1_PENALTY=4.878922 PRIMER_LEFT_1_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_1_SEQUENCE=GGGCGATTTAATATGGGTCA PRIMER_LEFT_1=49,22 PRIMER_RIGHT_1=1213,20 PRIMER_LEFT_1_TM=60.040 PRIMER_RIGHT_1_TM=59.621 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=4.500000 PRIMER_LEFT_1_END_STABILITY=5.7000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=2.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=1165 PRIMER_PAIR_2_PENALTY=7.515951 PRIMER_LEFT_2_PENALTY=1.909478 PRIMER_RIGHT_2_PENALTY=5.606474 PRIMER_LEFT_2_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_2_SEQUENCE=GGGCGATTTAATATGGGTCAT PRIMER_LEFT_2=49,21 PRIMER_RIGHT_2=1213,21 PRIMER_LEFT_2_TM=59.091 PRIMER_RIGHT_2_TM=59.894 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=4.500000 PRIMER_LEFT_2_END_STABILITY=5.7000 PRIMER_RIGHT_2_END_STABILITY=6.3000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=1165 PRIMER_PAIR_3_PENALTY=7.646353 PRIMER_LEFT_3_PENALTY=2.039879 PRIMER_RIGHT_3_PENALTY=5.606474 PRIMER_LEFT_3_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_3_SEQUENCE=GGGCGATTTAATATGGGTCAT PRIMER_LEFT_3=49,22 PRIMER_RIGHT_3=1213,21 PRIMER_LEFT_3_TM=60.040 PRIMER_RIGHT_3_TM=59.894 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=4.500000 PRIMER_LEFT_3_END_STABILITY=5.7000 PRIMER_RIGHT_3_END_STABILITY=6.3000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=1165 PRIMER_PAIR_4_PENALTY=8.353697 PRIMER_LEFT_4_PENALTY=1.909478 PRIMER_RIGHT_4_PENALTY=6.444219 PRIMER_LEFT_4_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_4_SEQUENCE=GGCGATTTAATATGGGTCATTC PRIMER_LEFT_4=49,21 PRIMER_RIGHT_4=1212,22 PRIMER_LEFT_4_TM=59.091 PRIMER_RIGHT_4_TM=59.556 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=4.000000 PRIMER_LEFT_4_END_STABILITY=5.7000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=1164 PRIMER_PAIR_5_PENALTY=8.431625 PRIMER_LEFT_5_PENALTY=1.909478 PRIMER_RIGHT_5_PENALTY=6.522147 PRIMER_LEFT_5_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_5_SEQUENCE=AGGGCGATTTAATATGGGTCA PRIMER_LEFT_5=49,21 PRIMER_RIGHT_5=1214,21 PRIMER_LEFT_5_TM=59.091 PRIMER_RIGHT_5_TM=60.522 PRIMER_LEFT_5_GC_PERCENT=47.619 PRIMER_RIGHT_5_GC_PERCENT=42.857 PRIMER_LEFT_5_SELF_ANY=6.00 PRIMER_RIGHT_5_SELF_ANY=5.00 PRIMER_LEFT_5_SELF_END=2.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=5.000000 PRIMER_LEFT_5_END_STABILITY=5.7000 PRIMER_RIGHT_5_END_STABILITY=7.9000 PRIMER_PAIR_5_COMPL_ANY=2.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=1166 PRIMER_PAIR_6_PENALTY=8.484099 PRIMER_LEFT_6_PENALTY=2.039879 PRIMER_RIGHT_6_PENALTY=6.444219 PRIMER_LEFT_6_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_6_SEQUENCE=GGCGATTTAATATGGGTCATTC PRIMER_LEFT_6=49,22 PRIMER_RIGHT_6=1212,22 PRIMER_LEFT_6_TM=60.040 PRIMER_RIGHT_6_TM=59.556 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=40.909 PRIMER_LEFT_6_SELF_ANY=6.00 PRIMER_RIGHT_6_SELF_ANY=5.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=4.000000 PRIMER_LEFT_6_END_STABILITY=5.7000 PRIMER_RIGHT_6_END_STABILITY=6.9000 PRIMER_PAIR_6_COMPL_ANY=3.00 PRIMER_PAIR_6_COMPL_END=2.00 PRIMER_PAIR_6_PRODUCT_SIZE=1164 PRIMER_PAIR_7_PENALTY=8.562026 PRIMER_LEFT_7_PENALTY=2.039879 PRIMER_RIGHT_7_PENALTY=6.522147 PRIMER_LEFT_7_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_7_SEQUENCE=AGGGCGATTTAATATGGGTCA PRIMER_LEFT_7=49,22 PRIMER_RIGHT_7=1214,21 PRIMER_LEFT_7_TM=60.040 PRIMER_RIGHT_7_TM=60.522 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=42.857 PRIMER_LEFT_7_SELF_ANY=6.00 PRIMER_RIGHT_7_SELF_ANY=5.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=5.000000 PRIMER_LEFT_7_END_STABILITY=5.7000 PRIMER_RIGHT_7_END_STABILITY=7.9000 PRIMER_PAIR_7_COMPL_ANY=2.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PAIR_7_PRODUCT_SIZE=1166 PRIMER_PAIR_8_PENALTY=8.579842 PRIMER_LEFT_8_PENALTY=1.909478 PRIMER_RIGHT_8_PENALTY=6.670364 PRIMER_LEFT_8_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_8_SEQUENCE=GCGATTTAATATGGGTCATTCA PRIMER_LEFT_8=49,21 PRIMER_RIGHT_8=1211,22 PRIMER_LEFT_8_TM=59.091 PRIMER_RIGHT_8_TM=58.830 PRIMER_LEFT_8_GC_PERCENT=47.619 PRIMER_RIGHT_8_GC_PERCENT=36.364 PRIMER_LEFT_8_SELF_ANY=6.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_LEFT_8_SELF_END=2.00 PRIMER_RIGHT_8_SELF_END=1.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=3.500000 PRIMER_LEFT_8_END_STABILITY=5.7000 PRIMER_RIGHT_8_END_STABILITY=6.9000 PRIMER_PAIR_8_COMPL_ANY=3.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PAIR_8_PRODUCT_SIZE=1163 PRIMER_PAIR_9_PENALTY=8.631677 PRIMER_LEFT_9_PENALTY=1.909478 PRIMER_RIGHT_9_PENALTY=6.722200 PRIMER_LEFT_9_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_9_SEQUENCE=CAAGGGCGATTTAATATGGG PRIMER_LEFT_9=49,21 PRIMER_RIGHT_9=1216,20 PRIMER_LEFT_9_TM=59.091 PRIMER_RIGHT_9_TM=59.278 PRIMER_LEFT_9_GC_PERCENT=47.619 PRIMER_RIGHT_9_GC_PERCENT=45.000 PRIMER_LEFT_9_SELF_ANY=6.00 PRIMER_RIGHT_9_SELF_ANY=5.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=6.000000 PRIMER_LEFT_9_END_STABILITY=5.7000 PRIMER_RIGHT_9_END_STABILITY=9.6000 PRIMER_PAIR_9_COMPL_ANY=2.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PAIR_9_PRODUCT_SIZE=1168 PRIMER_PAIR_10_PENALTY=8.710244 PRIMER_LEFT_10_PENALTY=2.039879 PRIMER_RIGHT_10_PENALTY=6.670364 PRIMER_LEFT_10_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_10_SEQUENCE=GCGATTTAATATGGGTCATTCA PRIMER_LEFT_10=49,22 PRIMER_RIGHT_10=1211,22 PRIMER_LEFT_10_TM=60.040 PRIMER_RIGHT_10_TM=58.830 PRIMER_LEFT_10_GC_PERCENT=50.000 PRIMER_RIGHT_10_GC_PERCENT=36.364 PRIMER_LEFT_10_SELF_ANY=6.00 PRIMER_RIGHT_10_SELF_ANY=5.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=3.500000 PRIMER_LEFT_10_END_STABILITY=5.7000 PRIMER_RIGHT_10_END_STABILITY=6.9000 PRIMER_PAIR_10_COMPL_ANY=3.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=1163 PRIMER_PAIR_11_PENALTY=8.762079 PRIMER_LEFT_11_PENALTY=2.039879 PRIMER_RIGHT_11_PENALTY=6.722200 PRIMER_LEFT_11_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_11_SEQUENCE=CAAGGGCGATTTAATATGGG PRIMER_LEFT_11=49,22 PRIMER_RIGHT_11=1216,20 PRIMER_LEFT_11_TM=60.040 PRIMER_RIGHT_11_TM=59.278 PRIMER_LEFT_11_GC_PERCENT=50.000 PRIMER_RIGHT_11_GC_PERCENT=45.000 PRIMER_LEFT_11_SELF_ANY=6.00 PRIMER_RIGHT_11_SELF_ANY=5.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=6.000000 PRIMER_LEFT_11_END_STABILITY=5.7000 PRIMER_RIGHT_11_END_STABILITY=9.6000 PRIMER_PAIR_11_COMPL_ANY=2.00 PRIMER_PAIR_11_COMPL_END=0.00 PRIMER_PAIR_11_PRODUCT_SIZE=1168 PRIMER_PAIR_12_PENALTY=8.958439 PRIMER_LEFT_12_PENALTY=1.909478 PRIMER_RIGHT_12_PENALTY=7.048962 PRIMER_LEFT_12_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_12_SEQUENCE=CAAGGGCGATTTAATATGGGT PRIMER_LEFT_12=49,21 PRIMER_RIGHT_12=1216,21 PRIMER_LEFT_12_TM=59.091 PRIMER_RIGHT_12_TM=60.049 PRIMER_LEFT_12_GC_PERCENT=47.619 PRIMER_RIGHT_12_GC_PERCENT=42.857 PRIMER_LEFT_12_SELF_ANY=6.00 PRIMER_RIGHT_12_SELF_ANY=5.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=6.000000 PRIMER_LEFT_12_END_STABILITY=5.7000 PRIMER_RIGHT_12_END_STABILITY=9.4000 PRIMER_PAIR_12_COMPL_ANY=2.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PAIR_12_PRODUCT_SIZE=1168 PRIMER_PAIR_13_PENALTY=9.013864 PRIMER_LEFT_13_PENALTY=1.909478 PRIMER_RIGHT_13_PENALTY=7.104387 PRIMER_LEFT_13_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_13_SEQUENCE=AGGGCGATTTAATATGGGTC PRIMER_LEFT_13=49,21 PRIMER_RIGHT_13=1214,20 PRIMER_LEFT_13_TM=59.091 PRIMER_RIGHT_13_TM=57.896 PRIMER_LEFT_13_GC_PERCENT=47.619 PRIMER_RIGHT_13_GC_PERCENT=45.000 PRIMER_LEFT_13_SELF_ANY=6.00 PRIMER_RIGHT_13_SELF_ANY=5.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=5.000000 PRIMER_LEFT_13_END_STABILITY=5.7000 PRIMER_RIGHT_13_END_STABILITY=9.1000 PRIMER_PAIR_13_COMPL_ANY=2.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PAIR_13_PRODUCT_SIZE=1166 PRIMER_PAIR_14_PENALTY=9.067167 PRIMER_LEFT_14_PENALTY=1.909478 PRIMER_RIGHT_14_PENALTY=7.157689 PRIMER_LEFT_14_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_14_SEQUENCE=GGCGATTTAATATGGGTCATT PRIMER_LEFT_14=49,21 PRIMER_RIGHT_14=1212,21 PRIMER_LEFT_14_TM=59.091 PRIMER_RIGHT_14_TM=57.842 PRIMER_LEFT_14_GC_PERCENT=47.619 PRIMER_RIGHT_14_GC_PERCENT=38.095 PRIMER_LEFT_14_SELF_ANY=6.00 PRIMER_RIGHT_14_SELF_ANY=5.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=4.000000 PRIMER_LEFT_14_END_STABILITY=5.7000 PRIMER_RIGHT_14_END_STABILITY=6.9000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PAIR_14_PRODUCT_SIZE=1164 PRIMER_PAIR_15_PENALTY=9.088841 PRIMER_LEFT_15_PENALTY=2.039879 PRIMER_RIGHT_15_PENALTY=7.048962 PRIMER_LEFT_15_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_15_SEQUENCE=CAAGGGCGATTTAATATGGGT PRIMER_LEFT_15=49,22 PRIMER_RIGHT_15=1216,21 PRIMER_LEFT_15_TM=60.040 PRIMER_RIGHT_15_TM=60.049 PRIMER_LEFT_15_GC_PERCENT=50.000 PRIMER_RIGHT_15_GC_PERCENT=42.857 PRIMER_LEFT_15_SELF_ANY=6.00 PRIMER_RIGHT_15_SELF_ANY=5.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=6.000000 PRIMER_LEFT_15_END_STABILITY=5.7000 PRIMER_RIGHT_15_END_STABILITY=9.4000 PRIMER_PAIR_15_COMPL_ANY=2.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=1168 PRIMER_PAIR_16_PENALTY=9.144266 PRIMER_LEFT_16_PENALTY=2.039879 PRIMER_RIGHT_16_PENALTY=7.104387 PRIMER_LEFT_16_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_16_SEQUENCE=AGGGCGATTTAATATGGGTC PRIMER_LEFT_16=49,22 PRIMER_RIGHT_16=1214,20 PRIMER_LEFT_16_TM=60.040 PRIMER_RIGHT_16_TM=57.896 PRIMER_LEFT_16_GC_PERCENT=50.000 PRIMER_RIGHT_16_GC_PERCENT=45.000 PRIMER_LEFT_16_SELF_ANY=6.00 PRIMER_RIGHT_16_SELF_ANY=5.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=2.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=5.000000 PRIMER_LEFT_16_END_STABILITY=5.7000 PRIMER_RIGHT_16_END_STABILITY=9.1000 PRIMER_PAIR_16_COMPL_ANY=2.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PAIR_16_PRODUCT_SIZE=1166 PRIMER_PAIR_17_PENALTY=9.197568 PRIMER_LEFT_17_PENALTY=2.039879 PRIMER_RIGHT_17_PENALTY=7.157689 PRIMER_LEFT_17_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_17_SEQUENCE=GGCGATTTAATATGGGTCATT PRIMER_LEFT_17=49,22 PRIMER_RIGHT_17=1212,21 PRIMER_LEFT_17_TM=60.040 PRIMER_RIGHT_17_TM=57.842 PRIMER_LEFT_17_GC_PERCENT=50.000 PRIMER_RIGHT_17_GC_PERCENT=38.095 PRIMER_LEFT_17_SELF_ANY=6.00 PRIMER_RIGHT_17_SELF_ANY=5.00 PRIMER_LEFT_17_SELF_END=2.00 PRIMER_RIGHT_17_SELF_END=1.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=4.000000 PRIMER_LEFT_17_END_STABILITY=5.7000 PRIMER_RIGHT_17_END_STABILITY=6.9000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PAIR_17_PRODUCT_SIZE=1164 PRIMER_PAIR_18_PENALTY=9.207691 PRIMER_LEFT_18_PENALTY=1.909478 PRIMER_RIGHT_18_PENALTY=7.298213 PRIMER_LEFT_18_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_18_SEQUENCE=AAGGGCGATTTAATATGGGTC PRIMER_LEFT_18=49,21 PRIMER_RIGHT_18=1215,21 PRIMER_LEFT_18_TM=59.091 PRIMER_RIGHT_18_TM=59.202 PRIMER_LEFT_18_GC_PERCENT=47.619 PRIMER_RIGHT_18_GC_PERCENT=42.857 PRIMER_LEFT_18_SELF_ANY=6.00 PRIMER_RIGHT_18_SELF_ANY=5.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=5.500000 PRIMER_LEFT_18_END_STABILITY=5.7000 PRIMER_RIGHT_18_END_STABILITY=9.1000 PRIMER_PAIR_18_COMPL_ANY=2.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PAIR_18_PRODUCT_SIZE=1167 PRIMER_PAIR_19_PENALTY=9.281084 PRIMER_LEFT_19_PENALTY=1.909478 PRIMER_RIGHT_19_PENALTY=7.371606 PRIMER_LEFT_19_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_19_SEQUENCE=GCGATTTAATATGGGTCATTCAT PRIMER_LEFT_19=49,21 PRIMER_RIGHT_19=1211,23 PRIMER_LEFT_19_TM=59.091 PRIMER_RIGHT_19_TM=59.128 PRIMER_LEFT_19_GC_PERCENT=47.619 PRIMER_RIGHT_19_GC_PERCENT=34.783 PRIMER_LEFT_19_SELF_ANY=6.00 PRIMER_RIGHT_19_SELF_ANY=5.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=3.500000 PRIMER_LEFT_19_END_STABILITY=5.7000 PRIMER_RIGHT_19_END_STABILITY=6.9000 PRIMER_PAIR_19_COMPL_ANY=3.00 PRIMER_PAIR_19_COMPL_END=2.00 PRIMER_PAIR_19_PRODUCT_SIZE=1163 = SEQUENCE_ID=L40146/UBC4 SEQUENCE_START_CODON_POSITION=0 SEQUENCE_TEMPLATE=ATGGCGCTGAAAAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACCAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAATGGACTCAGAAGTATGCGATGTAA PRIMER_LEFT_EXPLAIN=considered 3285, would not amplify any of the ORF 2182, GC content failed 11, low tm 330, high tm 255, high end compl 1, high 3' stability 7, ok 499 PRIMER_RIGHT_EXPLAIN=considered 3267, GC content failed 36, low tm 1201, high tm 523, high 3' stability 8, ok 1499 PRIMER_PAIR_EXPLAIN=considered 56, high end compl 34, ok 22 PRIMER_STOP_CODON_POSITION=441 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=6.820809 PRIMER_LEFT_0_PENALTY=4.584153 PRIMER_RIGHT_0_PENALTY=2.236656 PRIMER_LEFT_0_SEQUENCE=ATGGCGCTGAAAAGAATCCAC PRIMER_RIGHT_0_SEQUENCE=ACATCGCATACTTCTGAGTCCA PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=441,22 PRIMER_LEFT_0_TM=63.584 PRIMER_RIGHT_0_TM=59.763 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=442 PRIMER_PAIR_1_PENALTY=8.301465 PRIMER_LEFT_1_PENALTY=4.584153 PRIMER_RIGHT_1_PENALTY=3.717312 PRIMER_LEFT_1_SEQUENCE=ATGGCGCTGAAAAGAATCCAC PRIMER_RIGHT_1_SEQUENCE=TACATCGCATACTTCTGAGTCCA PRIMER_LEFT_1=0,21 PRIMER_RIGHT_1=442,23 PRIMER_LEFT_1_TM=63.584 PRIMER_RIGHT_1_TM=59.783 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=43.478 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.500000 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=443 PRIMER_PAIR_2_PENALTY=8.494006 PRIMER_LEFT_2_PENALTY=2.909495 PRIMER_RIGHT_2_PENALTY=5.584511 PRIMER_LEFT_2_SEQUENCE=ATGGCGCTGAAAAGAATCCA PRIMER_RIGHT_2_SEQUENCE=TTACATCGCATACTTCTGAGTCC PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=443,23 PRIMER_LEFT_2_TM=62.909 PRIMER_RIGHT_2_TM=58.415 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=1.000000 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=444 PRIMER_PAIR_3_PENALTY=10.168664 PRIMER_LEFT_3_PENALTY=4.584153 PRIMER_RIGHT_3_PENALTY=5.584511 PRIMER_LEFT_3_SEQUENCE=ATGGCGCTGAAAAGAATCCAC PRIMER_RIGHT_3_SEQUENCE=TTACATCGCATACTTCTGAGTCC PRIMER_LEFT_3=0,21 PRIMER_RIGHT_3=443,23 PRIMER_LEFT_3_TM=63.584 PRIMER_RIGHT_3_TM=58.415 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=1.000000 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=444 PRIMER_PAIR_4_PENALTY=10.604982 PRIMER_LEFT_4_PENALTY=4.584153 PRIMER_RIGHT_4_PENALTY=6.020829 PRIMER_LEFT_4_SEQUENCE=ATGGCGCTGAAAAGAATCCAC PRIMER_RIGHT_4_SEQUENCE=TTACATCGCATACTTCTGAGTCCA PRIMER_LEFT_4=0,21 PRIMER_RIGHT_4=443,24 PRIMER_LEFT_4_TM=63.584 PRIMER_RIGHT_4_TM=61.021 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=1.000000 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=444 PRIMER_PAIR_5_PENALTY=63.296939 PRIMER_LEFT_5_PENALTY=61.060283 PRIMER_RIGHT_5_PENALTY=2.236656 PRIMER_LEFT_5_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_5_SEQUENCE=ACATCGCATACTTCTGAGTCCA PRIMER_LEFT_5=3,19 PRIMER_RIGHT_5=441,22 PRIMER_LEFT_5_TM=59.940 PRIMER_RIGHT_5_TM=59.763 PRIMER_LEFT_5_GC_PERCENT=47.368 PRIMER_RIGHT_5_GC_PERCENT=45.455 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_POSITION_PENALTY=60.000000 PRIMER_RIGHT_5_POSITION_PENALTY=0.000000 PRIMER_LEFT_5_END_STABILITY=8.2000 PRIMER_RIGHT_5_END_STABILITY=7.9000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PAIR_5_PRODUCT_SIZE=439 PRIMER_PAIR_6_PENALTY=63.521548 PRIMER_LEFT_6_PENALTY=61.284892 PRIMER_RIGHT_6_PENALTY=2.236656 PRIMER_LEFT_6_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_6_SEQUENCE=ACATCGCATACTTCTGAGTCCA PRIMER_LEFT_6=3,20 PRIMER_RIGHT_6=441,22 PRIMER_LEFT_6_TM=61.285 PRIMER_RIGHT_6_TM=59.763 PRIMER_LEFT_6_GC_PERCENT=45.000 PRIMER_RIGHT_6_GC_PERCENT=45.455 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_POSITION_PENALTY=60.000000 PRIMER_RIGHT_6_POSITION_PENALTY=0.000000 PRIMER_LEFT_6_END_STABILITY=7.0000 PRIMER_RIGHT_6_END_STABILITY=7.9000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PAIR_6_PRODUCT_SIZE=439 PRIMER_PAIR_7_PENALTY=64.099814 PRIMER_LEFT_7_PENALTY=61.060283 PRIMER_RIGHT_7_PENALTY=3.039531 PRIMER_LEFT_7_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_7_SEQUENCE=ACATCGCATACTTCTGAGTCCAT PRIMER_LEFT_7=3,19 PRIMER_RIGHT_7=441,23 PRIMER_LEFT_7_TM=59.940 PRIMER_RIGHT_7_TM=60.040 PRIMER_LEFT_7_GC_PERCENT=47.368 PRIMER_RIGHT_7_GC_PERCENT=43.478 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=3.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_POSITION_PENALTY=60.000000 PRIMER_RIGHT_7_POSITION_PENALTY=0.000000 PRIMER_LEFT_7_END_STABILITY=8.2000 PRIMER_RIGHT_7_END_STABILITY=8.1000 PRIMER_PAIR_7_COMPL_ANY=5.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PAIR_7_PRODUCT_SIZE=439 PRIMER_PAIR_8_PENALTY=64.324423 PRIMER_LEFT_8_PENALTY=61.284892 PRIMER_RIGHT_8_PENALTY=3.039531 PRIMER_LEFT_8_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_8_SEQUENCE=ACATCGCATACTTCTGAGTCCAT PRIMER_LEFT_8=3,20 PRIMER_RIGHT_8=441,23 PRIMER_LEFT_8_TM=61.285 PRIMER_RIGHT_8_TM=60.040 PRIMER_LEFT_8_GC_PERCENT=45.000 PRIMER_RIGHT_8_GC_PERCENT=43.478 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=3.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_POSITION_PENALTY=60.000000 PRIMER_RIGHT_8_POSITION_PENALTY=0.000000 PRIMER_LEFT_8_END_STABILITY=7.0000 PRIMER_RIGHT_8_END_STABILITY=8.1000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PAIR_8_PRODUCT_SIZE=439 PRIMER_PAIR_9_PENALTY=64.777595 PRIMER_LEFT_9_PENALTY=61.060283 PRIMER_RIGHT_9_PENALTY=3.717312 PRIMER_LEFT_9_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_9_SEQUENCE=TACATCGCATACTTCTGAGTCCA PRIMER_LEFT_9=3,19 PRIMER_RIGHT_9=442,23 PRIMER_LEFT_9_TM=59.940 PRIMER_RIGHT_9_TM=59.783 PRIMER_LEFT_9_GC_PERCENT=47.368 PRIMER_RIGHT_9_GC_PERCENT=43.478 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=3.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=1.00 PRIMER_LEFT_9_POSITION_PENALTY=60.000000 PRIMER_RIGHT_9_POSITION_PENALTY=0.500000 PRIMER_LEFT_9_END_STABILITY=8.2000 PRIMER_RIGHT_9_END_STABILITY=7.9000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=2.00 PRIMER_PAIR_9_PRODUCT_SIZE=440 PRIMER_PAIR_10_PENALTY=65.002204 PRIMER_LEFT_10_PENALTY=61.284892 PRIMER_RIGHT_10_PENALTY=3.717312 PRIMER_LEFT_10_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_10_SEQUENCE=TACATCGCATACTTCTGAGTCCA PRIMER_LEFT_10=3,20 PRIMER_RIGHT_10=442,23 PRIMER_LEFT_10_TM=61.285 PRIMER_RIGHT_10_TM=59.783 PRIMER_LEFT_10_GC_PERCENT=45.000 PRIMER_RIGHT_10_GC_PERCENT=43.478 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=3.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=60.000000 PRIMER_RIGHT_10_POSITION_PENALTY=0.500000 PRIMER_LEFT_10_END_STABILITY=7.0000 PRIMER_RIGHT_10_END_STABILITY=7.9000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=440 PRIMER_PAIR_11_PENALTY=65.383808 PRIMER_LEFT_11_PENALTY=63.147152 PRIMER_RIGHT_11_PENALTY=2.236656 PRIMER_LEFT_11_SEQUENCE=GCGCTGAAAAGAATCCACAAG PRIMER_RIGHT_11_SEQUENCE=ACATCGCATACTTCTGAGTCCA PRIMER_LEFT_11=3,21 PRIMER_RIGHT_11=441,22 PRIMER_LEFT_11_TM=62.147 PRIMER_RIGHT_11_TM=59.763 PRIMER_LEFT_11_GC_PERCENT=47.619 PRIMER_RIGHT_11_GC_PERCENT=45.455 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=3.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=1.00 PRIMER_LEFT_11_POSITION_PENALTY=60.000000 PRIMER_RIGHT_11_POSITION_PENALTY=0.000000 PRIMER_LEFT_11_END_STABILITY=6.7000 PRIMER_RIGHT_11_END_STABILITY=7.9000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PAIR_11_PRODUCT_SIZE=439 PRIMER_PAIR_12_PENALTY=65.608414 PRIMER_LEFT_12_PENALTY=61.060283 PRIMER_RIGHT_12_PENALTY=4.548131 PRIMER_LEFT_12_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_12_SEQUENCE=TACATCGCATACTTCTGAGTCCAT PRIMER_LEFT_12=3,19 PRIMER_RIGHT_12=442,24 PRIMER_LEFT_12_TM=59.940 PRIMER_RIGHT_12_TM=60.048 PRIMER_LEFT_12_GC_PERCENT=47.368 PRIMER_RIGHT_12_GC_PERCENT=41.667 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=3.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=60.000000 PRIMER_RIGHT_12_POSITION_PENALTY=0.500000 PRIMER_LEFT_12_END_STABILITY=8.2000 PRIMER_RIGHT_12_END_STABILITY=8.1000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PAIR_12_PRODUCT_SIZE=440 PRIMER_PAIR_13_PENALTY=65.833022 PRIMER_LEFT_13_PENALTY=61.284892 PRIMER_RIGHT_13_PENALTY=4.548131 PRIMER_LEFT_13_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_13_SEQUENCE=TACATCGCATACTTCTGAGTCCAT PRIMER_LEFT_13=3,20 PRIMER_RIGHT_13=442,24 PRIMER_LEFT_13_TM=61.285 PRIMER_RIGHT_13_TM=60.048 PRIMER_LEFT_13_GC_PERCENT=45.000 PRIMER_RIGHT_13_GC_PERCENT=41.667 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=3.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=60.000000 PRIMER_RIGHT_13_POSITION_PENALTY=0.500000 PRIMER_LEFT_13_END_STABILITY=7.0000 PRIMER_RIGHT_13_END_STABILITY=8.1000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PAIR_13_PRODUCT_SIZE=440 PRIMER_PAIR_14_PENALTY=66.186683 PRIMER_LEFT_14_PENALTY=63.147152 PRIMER_RIGHT_14_PENALTY=3.039531 PRIMER_LEFT_14_SEQUENCE=GCGCTGAAAAGAATCCACAAG PRIMER_RIGHT_14_SEQUENCE=ACATCGCATACTTCTGAGTCCAT PRIMER_LEFT_14=3,21 PRIMER_RIGHT_14=441,23 PRIMER_LEFT_14_TM=62.147 PRIMER_RIGHT_14_TM=60.040 PRIMER_LEFT_14_GC_PERCENT=47.619 PRIMER_RIGHT_14_GC_PERCENT=43.478 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=3.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=60.000000 PRIMER_RIGHT_14_POSITION_PENALTY=0.000000 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=8.1000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=3.00 PRIMER_PAIR_14_PRODUCT_SIZE=439 PRIMER_PAIR_15_PENALTY=66.307348 PRIMER_LEFT_15_PENALTY=61.060283 PRIMER_RIGHT_15_PENALTY=5.247065 PRIMER_LEFT_15_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_15_SEQUENCE=ACATCGCATACTTCTGAGTCCATT PRIMER_LEFT_15=3,19 PRIMER_RIGHT_15=441,24 PRIMER_LEFT_15_TM=59.940 PRIMER_RIGHT_15_TM=61.247 PRIMER_LEFT_15_GC_PERCENT=47.368 PRIMER_RIGHT_15_GC_PERCENT=41.667 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=3.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=1.00 PRIMER_LEFT_15_POSITION_PENALTY=60.000000 PRIMER_RIGHT_15_POSITION_PENALTY=0.000000 PRIMER_LEFT_15_END_STABILITY=8.2000 PRIMER_RIGHT_15_END_STABILITY=8.4000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=2.00 PRIMER_PAIR_15_PRODUCT_SIZE=439 PRIMER_PAIR_16_PENALTY=66.531957 PRIMER_LEFT_16_PENALTY=61.284892 PRIMER_RIGHT_16_PENALTY=5.247065 PRIMER_LEFT_16_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_16_SEQUENCE=ACATCGCATACTTCTGAGTCCATT PRIMER_LEFT_16=3,20 PRIMER_RIGHT_16=441,24 PRIMER_LEFT_16_TM=61.285 PRIMER_RIGHT_16_TM=61.247 PRIMER_LEFT_16_GC_PERCENT=45.000 PRIMER_RIGHT_16_GC_PERCENT=41.667 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=3.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=1.00 PRIMER_LEFT_16_POSITION_PENALTY=60.000000 PRIMER_RIGHT_16_POSITION_PENALTY=0.000000 PRIMER_LEFT_16_END_STABILITY=7.0000 PRIMER_RIGHT_16_END_STABILITY=8.4000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=3.00 PRIMER_PAIR_16_PRODUCT_SIZE=439 PRIMER_PAIR_17_PENALTY=66.644794 PRIMER_LEFT_17_PENALTY=61.060283 PRIMER_RIGHT_17_PENALTY=5.584511 PRIMER_LEFT_17_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_17_SEQUENCE=TTACATCGCATACTTCTGAGTCC PRIMER_LEFT_17=3,19 PRIMER_RIGHT_17=443,23 PRIMER_LEFT_17_TM=59.940 PRIMER_RIGHT_17_TM=58.415 PRIMER_LEFT_17_GC_PERCENT=47.368 PRIMER_RIGHT_17_GC_PERCENT=43.478 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=3.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=1.00 PRIMER_LEFT_17_POSITION_PENALTY=60.000000 PRIMER_RIGHT_17_POSITION_PENALTY=1.000000 PRIMER_LEFT_17_END_STABILITY=8.2000 PRIMER_RIGHT_17_END_STABILITY=7.6000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PAIR_17_PRODUCT_SIZE=441 PRIMER_PAIR_18_PENALTY=66.864465 PRIMER_LEFT_18_PENALTY=63.147152 PRIMER_RIGHT_18_PENALTY=3.717312 PRIMER_LEFT_18_SEQUENCE=GCGCTGAAAAGAATCCACAAG PRIMER_RIGHT_18_SEQUENCE=TACATCGCATACTTCTGAGTCCA PRIMER_LEFT_18=3,21 PRIMER_RIGHT_18=442,23 PRIMER_LEFT_18_TM=62.147 PRIMER_RIGHT_18_TM=59.783 PRIMER_LEFT_18_GC_PERCENT=47.619 PRIMER_RIGHT_18_GC_PERCENT=43.478 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=3.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=1.00 PRIMER_LEFT_18_POSITION_PENALTY=60.000000 PRIMER_RIGHT_18_POSITION_PENALTY=0.500000 PRIMER_LEFT_18_END_STABILITY=6.7000 PRIMER_RIGHT_18_END_STABILITY=7.9000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PAIR_18_PRODUCT_SIZE=440 PRIMER_PAIR_19_PENALTY=66.869403 PRIMER_LEFT_19_PENALTY=61.284892 PRIMER_RIGHT_19_PENALTY=5.584511 PRIMER_LEFT_19_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_19_SEQUENCE=TTACATCGCATACTTCTGAGTCC PRIMER_LEFT_19=3,20 PRIMER_RIGHT_19=443,23 PRIMER_LEFT_19_TM=61.285 PRIMER_RIGHT_19_TM=58.415 PRIMER_LEFT_19_GC_PERCENT=45.000 PRIMER_RIGHT_19_GC_PERCENT=43.478 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=3.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=1.00 PRIMER_LEFT_19_POSITION_PENALTY=60.000000 PRIMER_RIGHT_19_POSITION_PENALTY=1.000000 PRIMER_LEFT_19_END_STABILITY=7.0000 PRIMER_RIGHT_19_END_STABILITY=7.6000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=2.00 PRIMER_PAIR_19_PRODUCT_SIZE=441 = SEQUENCE_ID=U51903/IQGAP2 SEQUENCE_START_CODON_POSITION=222 SEQUENCE_PRIMER=ATGCCACACGAAGAGCTGC SEQUENCE_TEMPLATE=GAGGGAGGAGAGTTCACTTTTACTTCAGTGTCAGCGCGCGGCGGCCGTGGCTGGCTCTGGCGAGAGAGCACCGAGGGAGTGGGTCGCAGATCTTCGGGCGGCTAGGGGAAATCGGCGAGAGGCGGGATCCGAGCGCGCCGGCGGGGCGCAGAGCCCGCGAGCCTGGCCAGCGAGGGTAGCCGCGGGGGGCGCGCCCCGGGCGGGCCCCCGGAGACGCGCAGGATGCCACACGAAGAGCTGCCGTCGCTGCAGAGACCCCGCTATGGCTCTATTGTGGACGATGAAAGGCTCTCTGCAGAGGAGATGGATGAGAGGAGGCGGCAGAACATTGCTTATGAATATCTGTGCCACTTAGAGGAAGCCAAAAGGTGGATGGAAGTTTGCTTAGTTGAAGAATTGCCACCAACCACTGAATTGGAAGAAGGGCTCCGGAATGGAGTTTACCTTGCAAAGTTAGCCAAGTTCTTTGCCCCGAAAATGGTATCAGAGAAAAAGATCTATGATGTGGAACAAACACGTTATAAGAAGTCTGGCCTTCATTTTCGACACACAGATAATACCGTCCAGTGGTTAAGAGCGATGGAGTCTATTGGTCTACCCAAGATATTTTATCCAGAAACAACAGATGTCTATGATCGGAAAAACATACCAAGAATGATATATTGCATTCACGCACTGAGTTTGTATCTGTTCAAACTAGGAATAGCACCCCAGATCCAGGATTTGTTGGGCAAAGTAGACTTCACAGAGGAGGAAATCAGTAATATGAGAAAAGAACTTGAGAAATATGGAATACAGATGCCATCTTTCAGCAAAATAGGTGGTATTCTGGCCAATGAACTGTCCGTGGATGAAGCTGCATTACATGCTGCAGTTATAGCCATTAATGAAGCAGTTGAAAAAGGAATAGCAGAGCAAACCGTTGTAACACTAAGAAACCCAAATGCGGTTTTAACTTTAGTGGATGACAACCTTGCACCAGAATATCAGAAAGAACTCTGGGATGCCAAAAAGAAAAAAGAGGAAAATGCAAGACTGAAGAATAGCTGTATTTCAGAAGAAGAAAGAGATGCTTATGAAGAACTGCTGACACAAGCAGAAATCCAAGGCAATATTAATAAAGTCAACAGGCAGGCTGCAGTGGACCATATCAATGCTGTCATTCCGGAAGGTGACCCCGAGAATACGCTGCTTGCACTGAAGAAACCAGAGGCCCAGCTGCCTGCTGTTTATCCCTTTGCTGCTGCCATGTATCAGAACGAACTTTTCAACCTCCAGAAACAGAACACCATGAACTACTTGGCCCACGAGGAGCTTTTGATTGCTGTGGAAATGTTGTCTGCTGTTGCTTTACTAAACCAGGCCTTGGAAAGCAACGATCTTGTGTCTGTGCAGAATCAACTCAGAAGCCCCGCAATAGGCTTAAACAATCTGGACAAGGCATATGTGGAACGTTATGCAAACACACTACTCTCTGTTAAACTAGAAGTTTTATCCCAAGGGCAAGATAACTTAAGCTGGAATGAAATTCAGAATTGTATTGATATGGTTAATGCTCAAATTCAAGAAGAAAATGACCGAGTTGTAGCTGTAGGGTACATCAATGAAGCTATTGATGAAGGGAATCCTTTGAGGACTTTAGAAACTTTGCTCCTACCTACTGCGAATATTAGTGATGTGGACCCAGCCCATGCCCAGCACTACCAGGATGTTTTATACCATGCTAAATCACAGAAACTCGGAGACTCTGAGAGTGTTTCCAAAGTGCTTTGGCTGGATGAGATACAGCAAGCCGTCGATGAGGCCAACGTGGACGAGGACAGAGCAAAACAATGGGTTACTCTGGTGGTTGATGTTAATCAGTGTTTGGAAGGAAAAAAATCAAGTGATATTTTGTCTGTATTGAAGTCTTCCACTTCTAATGCAAATGACATAATCCCGGAGTGTGCTGACAAATACTATGATGCCCTTGTGAAGGCAAAAGAGCTCAAATCTGAAAGAGTGTCTAGTGACGGTTCATGGCTCAAACTCAACCTGCACAAAAAATATGACTACTATTACAACACTGATTCAAAAGAGAGTTCCTGGGTCACACCTGAATCATGCTTCTATAAAGAATCATGGCTCACAGGAAAAGAAATCGAGGACATTATTGAGGAAGTCACAGTAGGTTACATTCGTGAGAATATATGGTCTGCTTCAGAAGAGTTGCTTCTTCGCTTTCAAGCCACAAGCTCAGGACCCATCCTTAGGGAAGAGTTTGAAGCTAGAAAATCATTTTTGCATGAACAAGAAGAGAATGTGGTCAAAATACAGGCTTTTTGGAAAGGATATAAACAACGGAAGGAGTATATGCACAGGCGGCAAACGTTCATTGATAATACTGATTCTGTTGTGAAGATTCAGTCCTGGTTCCGAATGGCAACTGCAAGAAAGAGCTATCTTTCAAGACTACAGTATTTCAGAGATCATAATAATGAAATTGTGAAAATACAGTCACTGTTGAGAGCGAACAAAGCTAGAGATGACTACAAAACATTGGTTGGCTCTGAAAACCCACCATTAACAGTAATTCGCAAATTTGTATACCTGCTGGACCAAAGTGATTTGGATTTCCAGGAGGAACTAGAGGTTGCACGATTAAGGGAAGAAGTAGTGACCAAGATCAGGGCCAATCAACAGCTGGAAAAAGACCTGAACCTGATGGACATCAAGATTGGACTGCTGGTGAAGAACAGGATCACACTAGAGGATGTAATTTCACACAGTAAAAAGCTGAACAAGAAAAAAGGAGGAGAAATGGAAATACTGAATAACACCGACAACCAAGGAATAAAAAGTTTGAGTAAGGAGAGGAGAAAAACACTAGAAACATATCAGCAGCTGTTTTACCTTTTACAGACCAACCCTTTATACTTGGCTAAGCTGATTTTCCAGATGCCACAGAACAAGTCCACTAAATTTATGGATACTGTTATTTTCACACTATATAATTATGCCTCTAATCAGCGAGAAGAATATCTACTTCTCAAGCTTTTTAAAACTGCTCTGGAGGAAGAAATAAAATCAAAAGTGGACCAGGTACAGGACATAGTTACTGGTAACCCTACAGTCATCAAGATGGTCGTCAGCTTCAATAGAGGTGCCCGGGGACAGAACACCCTGCGCCAACTCCTGGCTCCAGTGGTAAAAGAGATCATCGACGACAAGTCGCTGATTATCAACACAAACCCTGTAGAGGTGTACAAGGCTTGGGTGAACCAACTAGAAACACAGACTGGAGAGGCCAGCAAGTTGCCTTATGATGTGACCACAGAACAAGCTCTAACATACCCAGAAGTGAAAAATAAACTGGAGGCTTCCATTGAGAACCTGAGAAGGGTCACCGACAAAGTCCTGAATTCTATCATTTCTTCCCTTGATCTACTGCCTTATGGATTGAGGTATATAGCCAAAGTACTGAAGAATTCGATCCATGAGAAATTCCCCGATGCAACAGAAGATGAGCTATTAAAGATTGTTGGAAACCTCCTGTACTATCGGTACATGAATCCAGCCATTGTAGCTCCAGATGGCTTTGATATCATCGACATGACAGCTGGAGGTCAGATAAATTCTGACCAAAGGAGAAACTTAGGATCAGTGGCCAAGGTTCTTCAGCACGCAGCCTCCAACAAGCTGTTTGAAGGAGAAAATGAGCATCTCTCATCTATGAACAATTATTTATCAGAGACGTATCAGGAATTCAGGAAATATTTCAAAGAAGCATGTAATGTCCCTGAGCCAGAAGAGAAGTTTAATATGGACAAATACACAGACCTGGTGACAGTCAGCAAACCAGTCATTTATATTTCAATTGAAGAAATCATCAGCACACACTCACTCCTGTTGGAACACCAGGATGCAATTGCCCCTGAGAAAAATGACTTACTGAGTGAATTGCTGGGGTCGCTGGGAGAGGTGCCAACCGTGGAATCTTTTCTTGGGGAAGGAGCAGTTGACCCCAATGACCCTAACAAGGCAAATACACTAAGTCAGCTTTCAAAGACCGAGATTTCTCTTGTCTTGACAAGCAAATATGACATAGAGGACGGTGAAGCTATAGATAGCCGAAGCCTCATGATAAAGACCAAGAAGCTGATAATTGATGTGATCCGGAACCAGCCAGGGAACACATTGACAGAAATCTTAGAGACACCAGCAACTGCGCAACAGGAGGTAGACCATGCCACGGACATGGTGAGCCGTGCAATGATAGATTCCAGGACTCCAGAAGAAATGAAGCATAGCCAATCTATGATTGAAGATGCACAGCTGCCTCTTGAGCAGAAGAAGAGGAAAATCCAGAGGAATCTTCGGACGTTGGAACAGACTGGACACGTGTCATCCGAAAATAAATACCAAGACATTCTCAATGAGATTGCCAAGGATATTCGAAATCAAAGAATCTATCGTAAGCTTCGAAAAGCTGAATTGGCAAAACTTCAGCAGACCCTGAATGCACTTAACAAGAAGGCAGCATTTTATGAAGAGCAAATCAATTATTATGACACCTACATAAAGACTTGTTTAGACAACTTAAAAAGAAAAAATACTCGGAGATCAATTAAACTAGATGGAAAAGGAGAACCCAAAGGGGCGAAGAGAGCGAAGCCAGTGAAGTACACTGCAGCAAAGCTGCATGAGAAAGGTGTCCTGCTAGATATAGATGATCTTCAAACAAACCAGTTTAAGAATGTTACATTTGATATCATAGCTACTGAAGATGTAGGCATTTTCGATGTAAGATCAAAATTCCTTGGTGTTGAGATGGAAAAGGTGCAACTCAATATTCAGGATTTACTTCAGATGCAATATGAAGGAGTAGCTGTAATGAAAATGTTTGATAAGGTTAAAGTGAATGTAAACCTTCTCATATACCTGCTGAACAAGAAGTTCTATGGAAAGTGAAGTGCCTACAGAAATTTCTTGGATTCTGTATCATCTGGATTAGGAAATGAATTTGTTTAATATTTTTGTTTTTAAACATGATTGAAATCACTGCTTATAAATGTGTGATTTTTTTTAAATGACCAAAACTGTTCTGAAGAATGTACCCAGGTGCCTTTTTGCTAATTTGATACTATAATAGAATGAGACATAAAATGAATTAATGGAAACATATCCACACTGTACTGTGATATAGGTACTCTGATTTAAAACTTTGGACATCCTGTGATCTGTTTTAAAGTTGGGGGGTGGGAAATTTAGCTGACTAGGGACAAACATGTAAACCTATTTTCCTATGAAAAAAGTTTTAAATGTCCCACTTGAATAACGTAATTCTTCATAGTTTTTTTAATCTATGGATAAATGGAAACCTAATTATTTGTAATGAATTATTTAGACAGTTCTAAGCCCTGTCTTCTGGGAGTTATCAATTTTAAAGAGAACTTTTGTGCAATTCAAATGAAGTTTTTATAAGTAATTGAAAATGACAACACAATAACACTTTCTGTATAAAAGTATATATTTTATGTGATTTATTCCTACTAAATGAAAGTGCACTACTGCCTCATGTAAAGACTCTTGCACGCAGAGCCTTTAAGTGACTAAGGAACAACATAGATAGTGAGCATAGTCCCCACCTCCACCCCTCACAATTTATTTGAATACTTCAATTGTGCCTCTCAATTTTTTGTAATGCTAAAAAATCAGTATCTAGATGGTTTTTAAATGTATTCTCTGGAAATTGTTTTATGTAAAATAAATGTTACTTAATTCCATT PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 49099, GC content failed 2042, low tm 18100, high tm 10315, high end compl 16, long poly-x seq 226, high 3' stability 109, ok 18291 PRIMER_PAIR_EXPLAIN=considered 20, ok 20 PRIMER_STOP_CODON_POSITION=4947 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=10.109747 PRIMER_LEFT_0_PENALTY=3.573461 PRIMER_RIGHT_0_PENALTY=6.536286 PRIMER_LEFT_0_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_0_SEQUENCE=ACTTTCCATAGAACTTCTTGTTCAGC PRIMER_LEFT_0=222,19 PRIMER_RIGHT_0=4947,26 PRIMER_LEFT_0_TM=62.573 PRIMER_RIGHT_0_TM=60.536 PRIMER_LEFT_0_GC_PERCENT=57.895 PRIMER_RIGHT_0_GC_PERCENT=38.462 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=4726 PRIMER_PAIR_1_PENALTY=10.154611 PRIMER_LEFT_1_PENALTY=3.573461 PRIMER_RIGHT_1_PENALTY=6.581149 PRIMER_LEFT_1_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_1_SEQUENCE=CACTTTCCATAGAACTTCTTGTTCA PRIMER_LEFT_1=222,19 PRIMER_RIGHT_1=4948,25 PRIMER_LEFT_1_TM=62.573 PRIMER_RIGHT_1_TM=58.919 PRIMER_LEFT_1_GC_PERCENT=57.895 PRIMER_RIGHT_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.500000 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=4727 PRIMER_PAIR_2_PENALTY=11.102879 PRIMER_LEFT_2_PENALTY=3.573461 PRIMER_RIGHT_2_PENALTY=7.529417 PRIMER_LEFT_2_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_2_SEQUENCE=TCACTTTCCATAGAACTTCTTGTTCA PRIMER_LEFT_2=222,19 PRIMER_RIGHT_2=4949,26 PRIMER_LEFT_2_TM=62.573 PRIMER_RIGHT_2_TM=60.529 PRIMER_LEFT_2_GC_PERCENT=57.895 PRIMER_RIGHT_2_GC_PERCENT=34.615 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=1.000000 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=4728 PRIMER_PAIR_3_PENALTY=11.479573 PRIMER_LEFT_3_PENALTY=3.573461 PRIMER_RIGHT_3_PENALTY=7.906112 PRIMER_LEFT_3_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_3_SEQUENCE=TCACTTTCCATAGAACTTCTTGTTC PRIMER_LEFT_3=222,19 PRIMER_RIGHT_3=4949,25 PRIMER_LEFT_3_TM=62.573 PRIMER_RIGHT_3_TM=58.094 PRIMER_LEFT_3_GC_PERCENT=57.895 PRIMER_RIGHT_3_GC_PERCENT=36.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=1.000000 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=4728 PRIMER_PAIR_4_PENALTY=11.733380 PRIMER_LEFT_4_PENALTY=3.573461 PRIMER_RIGHT_4_PENALTY=8.159919 PRIMER_LEFT_4_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_4_SEQUENCE=GGCACTTCACTTTCCATAGAACT PRIMER_LEFT_4=222,19 PRIMER_RIGHT_4=4955,23 PRIMER_LEFT_4_TM=62.573 PRIMER_RIGHT_4_TM=58.840 PRIMER_LEFT_4_GC_PERCENT=57.895 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=4.000000 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=4734 PRIMER_PAIR_5_PENALTY=11.773031 PRIMER_LEFT_5_PENALTY=3.573461 PRIMER_RIGHT_5_PENALTY=8.199569 PRIMER_LEFT_5_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_5_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTTC PRIMER_LEFT_5=222,19 PRIMER_RIGHT_5=4950,26 PRIMER_LEFT_5_TM=62.573 PRIMER_RIGHT_5_TM=59.300 PRIMER_LEFT_5_GC_PERCENT=57.895 PRIMER_RIGHT_5_GC_PERCENT=34.615 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=7.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=1.500000 PRIMER_LEFT_5_END_STABILITY=9.7000 PRIMER_RIGHT_5_END_STABILITY=6.7000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PAIR_5_PRODUCT_SIZE=4729 PRIMER_PAIR_6_PENALTY=12.340390 PRIMER_LEFT_6_PENALTY=3.573461 PRIMER_RIGHT_6_PENALTY=8.766928 PRIMER_LEFT_6_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_6_SEQUENCE=AGGCACTTCACTTTCCATAGAACT PRIMER_LEFT_6=222,19 PRIMER_RIGHT_6=4956,24 PRIMER_LEFT_6_TM=62.573 PRIMER_RIGHT_6_TM=59.733 PRIMER_LEFT_6_GC_PERCENT=57.895 PRIMER_RIGHT_6_GC_PERCENT=41.667 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=4.500000 PRIMER_LEFT_6_END_STABILITY=9.7000 PRIMER_RIGHT_6_END_STABILITY=6.4000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PAIR_6_PRODUCT_SIZE=4735 PRIMER_PAIR_7_PENALTY=12.381077 PRIMER_LEFT_7_PENALTY=3.573461 PRIMER_RIGHT_7_PENALTY=8.807615 PRIMER_LEFT_7_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_7_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTT PRIMER_LEFT_7=222,19 PRIMER_RIGHT_7=4950,25 PRIMER_LEFT_7_TM=62.573 PRIMER_RIGHT_7_TM=57.692 PRIMER_LEFT_7_GC_PERCENT=57.895 PRIMER_RIGHT_7_GC_PERCENT=32.000 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=7.00 PRIMER_LEFT_7_SELF_END=3.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=1.500000 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=7.0000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PAIR_7_PRODUCT_SIZE=4729 PRIMER_PAIR_8_PENALTY=12.659800 PRIMER_LEFT_8_PENALTY=3.573461 PRIMER_RIGHT_8_PENALTY=9.086339 PRIMER_LEFT_8_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_8_SEQUENCE=TCTGTAGGCACTTCACTTTCCA PRIMER_LEFT_8=222,19 PRIMER_RIGHT_8=4961,22 PRIMER_LEFT_8_TM=62.573 PRIMER_RIGHT_8_TM=59.914 PRIMER_LEFT_8_GC_PERCENT=57.895 PRIMER_RIGHT_8_GC_PERCENT=45.455 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=4.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=7.000000 PRIMER_LEFT_8_END_STABILITY=9.7000 PRIMER_RIGHT_8_END_STABILITY=8.5000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PAIR_8_PRODUCT_SIZE=4740 PRIMER_PAIR_9_PENALTY=12.674341 PRIMER_LEFT_9_PENALTY=3.573461 PRIMER_RIGHT_9_PENALTY=9.100880 PRIMER_LEFT_9_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_9_SEQUENCE=CTTCACTTTCCATAGAACTTCTTGTTC PRIMER_LEFT_9=222,19 PRIMER_RIGHT_9=4951,27 PRIMER_LEFT_9_TM=62.573 PRIMER_RIGHT_9_TM=60.101 PRIMER_LEFT_9_GC_PERCENT=57.895 PRIMER_RIGHT_9_GC_PERCENT=37.037 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=7.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=2.000000 PRIMER_LEFT_9_END_STABILITY=9.7000 PRIMER_RIGHT_9_END_STABILITY=6.7000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=2.00 PRIMER_PAIR_9_PRODUCT_SIZE=4730 PRIMER_PAIR_10_PENALTY=12.997281 PRIMER_LEFT_10_PENALTY=3.573461 PRIMER_RIGHT_10_PENALTY=9.423820 PRIMER_LEFT_10_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_10_SEQUENCE=CTTCACTTTCCATAGAACTTCTTGTT PRIMER_LEFT_10=222,19 PRIMER_RIGHT_10=4951,26 PRIMER_LEFT_10_TM=62.573 PRIMER_RIGHT_10_TM=58.576 PRIMER_LEFT_10_GC_PERCENT=57.895 PRIMER_RIGHT_10_GC_PERCENT=34.615 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=7.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=3.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=2.000000 PRIMER_LEFT_10_END_STABILITY=9.7000 PRIMER_RIGHT_10_END_STABILITY=7.0000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=3.00 PRIMER_PAIR_10_PRODUCT_SIZE=4730 PRIMER_PAIR_11_PENALTY=13.050739 PRIMER_LEFT_11_PENALTY=3.573461 PRIMER_RIGHT_11_PENALTY=9.477278 PRIMER_LEFT_11_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_11_SEQUENCE=CTGTAGGCACTTCACTTTCCA PRIMER_LEFT_11=222,19 PRIMER_RIGHT_11=4960,21 PRIMER_LEFT_11_TM=62.573 PRIMER_RIGHT_11_TM=58.023 PRIMER_LEFT_11_GC_PERCENT=57.895 PRIMER_RIGHT_11_GC_PERCENT=47.619 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=3.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=6.500000 PRIMER_LEFT_11_END_STABILITY=9.7000 PRIMER_RIGHT_11_END_STABILITY=8.5000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PAIR_11_PRODUCT_SIZE=4739 PRIMER_PAIR_12_PENALTY=13.229460 PRIMER_LEFT_12_PENALTY=3.573461 PRIMER_RIGHT_12_PENALTY=9.655999 PRIMER_LEFT_12_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_12_SEQUENCE=TGTAGGCACTTCACTTTCCAT PRIMER_LEFT_12=222,19 PRIMER_RIGHT_12=4959,21 PRIMER_LEFT_12_TM=62.573 PRIMER_RIGHT_12_TM=57.344 PRIMER_LEFT_12_GC_PERCENT=57.895 PRIMER_RIGHT_12_GC_PERCENT=42.857 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=3.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=6.000000 PRIMER_LEFT_12_END_STABILITY=9.7000 PRIMER_RIGHT_12_END_STABILITY=8.1000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PAIR_12_PRODUCT_SIZE=4738 PRIMER_PAIR_13_PENALTY=13.336123 PRIMER_LEFT_13_PENALTY=3.573461 PRIMER_RIGHT_13_PENALTY=9.762661 PRIMER_LEFT_13_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_13_SEQUENCE=ACTTTCCATAGAACTTCTTGTTCAGCA PRIMER_LEFT_13=222,19 PRIMER_RIGHT_13=4947,27 PRIMER_LEFT_13_TM=62.573 PRIMER_RIGHT_13_TM=62.763 PRIMER_LEFT_13_GC_PERCENT=57.895 PRIMER_RIGHT_13_GC_PERCENT=37.037 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_LEFT_13_SELF_END=3.00 PRIMER_RIGHT_13_SELF_END=3.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=0.000000 PRIMER_LEFT_13_END_STABILITY=9.7000 PRIMER_RIGHT_13_END_STABILITY=8.5000 PRIMER_PAIR_13_COMPL_ANY=6.00 PRIMER_PAIR_13_COMPL_END=3.00 PRIMER_PAIR_13_PRODUCT_SIZE=4726 PRIMER_PAIR_14_PENALTY=13.671473 PRIMER_LEFT_14_PENALTY=3.573461 PRIMER_RIGHT_14_PENALTY=10.098012 PRIMER_LEFT_14_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_14_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTTCA PRIMER_LEFT_14=222,19 PRIMER_RIGHT_14=4950,27 PRIMER_LEFT_14_TM=62.573 PRIMER_RIGHT_14_TM=61.598 PRIMER_LEFT_14_GC_PERCENT=57.895 PRIMER_RIGHT_14_GC_PERCENT=33.333 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=7.00 PRIMER_LEFT_14_SELF_END=3.00 PRIMER_RIGHT_14_SELF_END=3.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=1.500000 PRIMER_LEFT_14_END_STABILITY=9.7000 PRIMER_RIGHT_14_END_STABILITY=6.7000 PRIMER_PAIR_14_COMPL_ANY=6.00 PRIMER_PAIR_14_COMPL_END=3.00 PRIMER_PAIR_14_PRODUCT_SIZE=4729 PRIMER_PAIR_15_PENALTY=13.678404 PRIMER_LEFT_15_PENALTY=3.573461 PRIMER_RIGHT_15_PENALTY=10.104943 PRIMER_LEFT_15_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_15_SEQUENCE=CTGTAGGCACTTCACTTTCCAT PRIMER_LEFT_15=222,19 PRIMER_RIGHT_15=4960,22 PRIMER_LEFT_15_TM=62.573 PRIMER_RIGHT_15_TM=58.395 PRIMER_LEFT_15_GC_PERCENT=57.895 PRIMER_RIGHT_15_GC_PERCENT=45.455 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=4.00 PRIMER_LEFT_15_SELF_END=3.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=6.500000 PRIMER_LEFT_15_END_STABILITY=9.7000 PRIMER_RIGHT_15_END_STABILITY=8.1000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=3.00 PRIMER_PAIR_15_PRODUCT_SIZE=4739 PRIMER_PAIR_16_PENALTY=13.751887 PRIMER_LEFT_16_PENALTY=3.573461 PRIMER_RIGHT_16_PENALTY=10.178426 PRIMER_LEFT_16_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_16_SEQUENCE=TCTGTAGGCACTTCACTTTCCAT PRIMER_LEFT_16=222,19 PRIMER_RIGHT_16=4961,23 PRIMER_LEFT_16_TM=62.573 PRIMER_RIGHT_16_TM=60.178 PRIMER_LEFT_16_GC_PERCENT=57.895 PRIMER_RIGHT_16_GC_PERCENT=43.478 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=2.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=7.000000 PRIMER_LEFT_16_END_STABILITY=9.7000 PRIMER_RIGHT_16_END_STABILITY=8.1000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=3.00 PRIMER_PAIR_16_PRODUCT_SIZE=4740 PRIMER_PAIR_17_PENALTY=13.790485 PRIMER_LEFT_17_PENALTY=3.573461 PRIMER_RIGHT_17_PENALTY=10.217023 PRIMER_LEFT_17_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_17_SEQUENCE=ACTTCACTTTCCATAGAACTTCTTGTT PRIMER_LEFT_17=222,19 PRIMER_RIGHT_17=4952,27 PRIMER_LEFT_17_TM=62.573 PRIMER_RIGHT_17_TM=59.283 PRIMER_LEFT_17_GC_PERCENT=57.895 PRIMER_RIGHT_17_GC_PERCENT=33.333 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=7.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=3.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=2.500000 PRIMER_LEFT_17_END_STABILITY=9.7000 PRIMER_RIGHT_17_END_STABILITY=7.0000 PRIMER_PAIR_17_COMPL_ANY=6.00 PRIMER_PAIR_17_COMPL_END=3.00 PRIMER_PAIR_17_PRODUCT_SIZE=4731 PRIMER_PAIR_18_PENALTY=13.822383 PRIMER_LEFT_18_PENALTY=3.573461 PRIMER_RIGHT_18_PENALTY=10.248922 PRIMER_LEFT_18_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_18_SEQUENCE=TAGGCACTTCACTTTCCATAGAACT PRIMER_LEFT_18=222,19 PRIMER_RIGHT_18=4957,25 PRIMER_LEFT_18_TM=62.573 PRIMER_RIGHT_18_TM=59.751 PRIMER_LEFT_18_GC_PERCENT=57.895 PRIMER_RIGHT_18_GC_PERCENT=40.000 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=6.00 PRIMER_LEFT_18_SELF_END=3.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=5.000000 PRIMER_LEFT_18_END_STABILITY=9.7000 PRIMER_RIGHT_18_END_STABILITY=6.4000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PAIR_18_PRODUCT_SIZE=4736 PRIMER_PAIR_19_PENALTY=13.836123 PRIMER_LEFT_19_PENALTY=3.573461 PRIMER_RIGHT_19_PENALTY=10.262661 PRIMER_LEFT_19_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_19_SEQUENCE=CACTTTCCATAGAACTTCTTGTTCAGC PRIMER_LEFT_19=222,19 PRIMER_RIGHT_19=4948,27 PRIMER_LEFT_19_TM=62.573 PRIMER_RIGHT_19_TM=62.763 PRIMER_LEFT_19_GC_PERCENT=57.895 PRIMER_RIGHT_19_GC_PERCENT=40.741 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=7.00 PRIMER_LEFT_19_SELF_END=3.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=0.500000 PRIMER_LEFT_19_END_STABILITY=9.7000 PRIMER_RIGHT_19_END_STABILITY=8.2000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PAIR_19_PRODUCT_SIZE=4727 = SEQUENCE_ID=M59040/CD44 P3_COMMENT=Human cell adhesion molecule (CD44) mRNA, complete cds. CDS_29..1114 SEQUENCE_START_CODON_POSITION=28 SEQUENCE_TEMPLATE=CCCGCGCCCTCCGTTCGCTCCGGACACCATGGACAAGTTTTGGTGGCACGCAGCCTGGGGACTCTGCCTCGTGCCGCTGAGCCTGGCGCAGATCGATTTGAATATAACCTGCCGCTTTGCAGGTGTATTCCACGTGGAGAAAAATGGTCGCTACAGCATCTCTCGGACGGAGGCCGCTGACCTCTGCAAGGCTTTCAATAGCACCTTGCCCACAATGGCCCAGATGGAGAAAGCTCTGAGCATCGGATTTGAGACCTGCAGGTATGGGTTCATAGAAGGGCATGTGGTGATTCCCCGGATCCACCCCAACTCCATCTGTGCAGCAAACAACACAGGGGTGTACATCCTCACATACAACACCTCCCAGTATGACACATATTGCTTCAATGCTTCAGCTCCACCTGAAGAAGATTGTACATCAGTCACAGACCTGCCCAATGCCTTTGATGGACCAATTACCATAACTATTGTTAACCGTGATGGCACCCGCTATGTCCAGAAAGGAGAATACAGAACGAATCCTGAAGACATCTACCCCAGCAACCCTACTGATGATGACGTGAGCAGCGGCTCCTCCAGTGAAAGGAGCAGCACTTCAGGAGGTTACATCTTTTACACCTTTTCTACTGTACACCCCATCCCAGACGAAGACAGTCCCTGGATCACCGACAGCACAGACAGAATCCCTGCTACCAGAGACCAAGACACATTCCACCCCAGTGGGGGGTCCCATACCACTCATGGATCTGAATCAGATGGACACTCACATGGGAGTCAAGAAGGTGGAGCAAACACAACCTCTGGTCCTATAAGGACACCCCAAATTCCAGAATGGCTGATCATCTTGGCATCCCTCTTGGCCTTGGCTTTGATTCTTGCAGTTTGCATTGCAGTCAACAGTCGAAGAAGGTGTGGGCAGAAGAAAAAGCTAGTGATCAACAGTGGCAATGGAGCTGTGGAGGACAGAAAGCCAAGTGGACTCAACGGAGAGGCCAGCAAGTCTCAGGAAATGGTGCATTTGGTGAACAAGGAGTCGTCAGAAACTCCAGACCAGTTTATGACAGCTGATGAGACAAGGAACCTGCAGAATGTGGACATGAAGATTGGGGTGTAACACCTACACCATTATCTTGGAAAGAAACAACCGTTGGAAACATAACCATTACAGGGAGCTGGGACACTTAACAGATGCAATGTGCTACTGATTGTTTCATTGCGAATCTTTTTTAGCATAAAATTTTCTACTCTTTTTGTTTTTTGTGTTTTGTTCTTTAAAGTCAGGTCCAATTTGTAAAAACAGCATTGCTTTGTAAATTAGGGCCCAATTAATAATCAGCAAGAATTTGATCGTTCAGTTCCACTTGGAGGCCTTCATCCTCGGGTGTGCTATGGATGGCTTCTAACAAAAACTACACATATGTATTCCTGATCGCCAACCTTTCCCCCACCAGCTAAGGACATTTCCCAGGGTTAATAGGGCCTGGTCCCTGGGAGGAAATTTGAATGGGTCCATTTTGCCCTTCCATAGCCTAATCCCTGGGCATTGCTTTCCACTGAGGTTGGGGTGTACTAGTTACACATCTTCAACAGACCCCCTCTAGAAATTTTTCAGATGCTTCTGGGAGACACCAAAGGGTGAAGCTATTTATCTGTAGTAAACTATTTATCTGTGTTTTTGAAATATTAAACCCTGGATCAGTCCTTTGATCAGTATAATTTTTTAAAGTTACTTTGTCAGAGGCACAAAAGGGTTTAAACTGATTCATAATAAATATCTGTACTTCTTCGATCTTC PRIMER_LEFT_EXPLAIN=considered 15178, would not amplify any of the ORF 11930, GC content failed 2, low tm 495, high tm 1516, high end compl 1, long poly-x seq 22, high 3' stability 27, ok 1185 PRIMER_RIGHT_EXPLAIN=considered 14630, GC content failed 362, low tm 3262, high tm 5355, high any compl 3, high end compl 2, long poly-x seq 73, high 3' stability 60, ok 5513 PRIMER_PAIR_EXPLAIN=considered 22, high end compl 1, ok 21 PRIMER_STOP_CODON_POSITION=1111 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=1.699596 PRIMER_LEFT_0_PENALTY=1.335843 PRIMER_RIGHT_0_PENALTY=0.363753 PRIMER_LEFT_0_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_0_SEQUENCE=ACACCCCAATCTTCATGTCC PRIMER_LEFT_0=28,20 PRIMER_RIGHT_0=1111,20 PRIMER_LEFT_0_TM=61.336 PRIMER_RIGHT_0_TM=59.636 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=1084 PRIMER_PAIR_1_PENALTY=2.962375 PRIMER_LEFT_1_PENALTY=2.598622 PRIMER_RIGHT_1_PENALTY=0.363753 PRIMER_LEFT_1_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_1_SEQUENCE=ACACCCCAATCTTCATGTCC PRIMER_LEFT_1=28,19 PRIMER_RIGHT_1=1111,20 PRIMER_LEFT_1_TM=58.401 PRIMER_RIGHT_1_TM=59.636 PRIMER_LEFT_1_GC_PERCENT=47.368 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=1084 PRIMER_PAIR_2_PENALTY=3.174248 PRIMER_LEFT_2_PENALTY=1.335843 PRIMER_RIGHT_2_PENALTY=1.838405 PRIMER_LEFT_2_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_2_SEQUENCE=TACACCCCAATCTTCATGTCC PRIMER_LEFT_2=28,20 PRIMER_RIGHT_2=1112,21 PRIMER_LEFT_2_TM=61.336 PRIMER_RIGHT_2_TM=59.662 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=0.500000 PRIMER_LEFT_2_END_STABILITY=10.0000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=1085 PRIMER_PAIR_3_PENALTY=4.437027 PRIMER_LEFT_3_PENALTY=2.598622 PRIMER_RIGHT_3_PENALTY=1.838405 PRIMER_LEFT_3_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_3_SEQUENCE=TACACCCCAATCTTCATGTCC PRIMER_LEFT_3=28,19 PRIMER_RIGHT_3=1112,21 PRIMER_LEFT_3_TM=58.401 PRIMER_RIGHT_3_TM=59.662 PRIMER_LEFT_3_GC_PERCENT=47.368 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.500000 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=1085 PRIMER_PAIR_4_PENALTY=4.849987 PRIMER_LEFT_4_PENALTY=1.335843 PRIMER_RIGHT_4_PENALTY=3.514144 PRIMER_LEFT_4_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_4_SEQUENCE=ACACCCCAATCTTCATGTCCA PRIMER_LEFT_4=28,20 PRIMER_RIGHT_4=1111,21 PRIMER_LEFT_4_TM=61.336 PRIMER_RIGHT_4_TM=62.514 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=0.000000 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=1084 PRIMER_PAIR_5_PENALTY=5.303759 PRIMER_LEFT_5_PENALTY=1.335843 PRIMER_RIGHT_5_PENALTY=3.967916 PRIMER_LEFT_5_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_5_SEQUENCE=TTACACCCCAATCTTCATGTCC PRIMER_LEFT_5=28,20 PRIMER_RIGHT_5=1113,22 PRIMER_LEFT_5_TM=61.336 PRIMER_RIGHT_5_TM=60.968 PRIMER_LEFT_5_GC_PERCENT=45.000 PRIMER_RIGHT_5_GC_PERCENT=45.455 PRIMER_LEFT_5_SELF_ANY=3.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=1.000000 PRIMER_LEFT_5_END_STABILITY=10.0000 PRIMER_RIGHT_5_END_STABILITY=7.9000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PAIR_5_PRODUCT_SIZE=1086 PRIMER_PAIR_6_PENALTY=5.959170 PRIMER_LEFT_6_PENALTY=1.335843 PRIMER_RIGHT_6_PENALTY=4.623327 PRIMER_LEFT_6_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_6_SEQUENCE=TTACACCCCAATCTTCATGTC PRIMER_LEFT_6=28,20 PRIMER_RIGHT_6=1113,21 PRIMER_LEFT_6_TM=61.336 PRIMER_RIGHT_6_TM=57.377 PRIMER_LEFT_6_GC_PERCENT=45.000 PRIMER_RIGHT_6_GC_PERCENT=42.857 PRIMER_LEFT_6_SELF_ANY=3.00 PRIMER_RIGHT_6_SELF_ANY=4.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=1.000000 PRIMER_LEFT_6_END_STABILITY=10.0000 PRIMER_RIGHT_6_END_STABILITY=6.3000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=2.00 PRIMER_PAIR_6_PRODUCT_SIZE=1086 PRIMER_PAIR_7_PENALTY=6.112765 PRIMER_LEFT_7_PENALTY=2.598622 PRIMER_RIGHT_7_PENALTY=3.514144 PRIMER_LEFT_7_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_7_SEQUENCE=ACACCCCAATCTTCATGTCCA PRIMER_LEFT_7=28,19 PRIMER_RIGHT_7=1111,21 PRIMER_LEFT_7_TM=58.401 PRIMER_RIGHT_7_TM=62.514 PRIMER_LEFT_7_GC_PERCENT=47.368 PRIMER_RIGHT_7_GC_PERCENT=47.619 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=0.000000 PRIMER_LEFT_7_END_STABILITY=9.4000 PRIMER_RIGHT_7_END_STABILITY=7.9000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PAIR_7_PRODUCT_SIZE=1084 PRIMER_PAIR_8_PENALTY=6.117351 PRIMER_LEFT_8_PENALTY=1.335843 PRIMER_RIGHT_8_PENALTY=4.781508 PRIMER_LEFT_8_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_8_SEQUENCE=TGTTACACCCCAATCTTCATGT PRIMER_LEFT_8=28,20 PRIMER_RIGHT_8=1115,22 PRIMER_LEFT_8_TM=61.336 PRIMER_RIGHT_8_TM=59.218 PRIMER_LEFT_8_GC_PERCENT=45.000 PRIMER_RIGHT_8_GC_PERCENT=40.909 PRIMER_LEFT_8_SELF_ANY=3.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=2.000000 PRIMER_LEFT_8_END_STABILITY=10.0000 PRIMER_RIGHT_8_END_STABILITY=6.6000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PAIR_8_PRODUCT_SIZE=1088 PRIMER_PAIR_9_PENALTY=6.264997 PRIMER_LEFT_9_PENALTY=1.335843 PRIMER_RIGHT_9_PENALTY=4.929154 PRIMER_LEFT_9_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_9_SEQUENCE=TACACCCCAATCTTCATGTCCA PRIMER_LEFT_9=28,20 PRIMER_RIGHT_9=1112,22 PRIMER_LEFT_9_TM=61.336 PRIMER_RIGHT_9_TM=62.429 PRIMER_LEFT_9_GC_PERCENT=45.000 PRIMER_RIGHT_9_GC_PERCENT=45.455 PRIMER_LEFT_9_SELF_ANY=3.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=0.500000 PRIMER_LEFT_9_END_STABILITY=10.0000 PRIMER_RIGHT_9_END_STABILITY=7.9000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=3.00 PRIMER_PAIR_9_PRODUCT_SIZE=1085 PRIMER_PAIR_10_PENALTY=6.542885 PRIMER_LEFT_10_PENALTY=1.335843 PRIMER_RIGHT_10_PENALTY=5.207042 PRIMER_LEFT_10_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_10_SEQUENCE=GTTACACCCCAATCTTCATGTC PRIMER_LEFT_10=28,20 PRIMER_RIGHT_10=1114,22 PRIMER_LEFT_10_TM=61.336 PRIMER_RIGHT_10_TM=58.293 PRIMER_LEFT_10_GC_PERCENT=45.000 PRIMER_RIGHT_10_GC_PERCENT=45.455 PRIMER_LEFT_10_SELF_ANY=3.00 PRIMER_RIGHT_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=1.500000 PRIMER_LEFT_10_END_STABILITY=10.0000 PRIMER_RIGHT_10_END_STABILITY=6.3000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PAIR_10_PRODUCT_SIZE=1087 PRIMER_PAIR_11_PENALTY=6.566538 PRIMER_LEFT_11_PENALTY=2.598622 PRIMER_RIGHT_11_PENALTY=3.967916 PRIMER_LEFT_11_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_11_SEQUENCE=TTACACCCCAATCTTCATGTCC PRIMER_LEFT_11=28,19 PRIMER_RIGHT_11=1113,22 PRIMER_LEFT_11_TM=58.401 PRIMER_RIGHT_11_TM=60.968 PRIMER_LEFT_11_GC_PERCENT=47.368 PRIMER_RIGHT_11_GC_PERCENT=45.455 PRIMER_LEFT_11_SELF_ANY=3.00 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=1.000000 PRIMER_LEFT_11_END_STABILITY=9.4000 PRIMER_RIGHT_11_END_STABILITY=7.9000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PAIR_11_PRODUCT_SIZE=1086 PRIMER_PAIR_12_PENALTY=6.868587 PRIMER_LEFT_12_PENALTY=1.335843 PRIMER_RIGHT_12_PENALTY=5.532744 PRIMER_LEFT_12_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_12_SEQUENCE=GTGTTACACCCCAATCTTCATGT PRIMER_LEFT_12=28,20 PRIMER_RIGHT_12=1116,23 PRIMER_LEFT_12_TM=61.336 PRIMER_RIGHT_12_TM=60.033 PRIMER_LEFT_12_GC_PERCENT=45.000 PRIMER_RIGHT_12_GC_PERCENT=43.478 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=7.00 PRIMER_LEFT_12_SELF_END=3.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=2.500000 PRIMER_LEFT_12_END_STABILITY=10.0000 PRIMER_RIGHT_12_END_STABILITY=6.6000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PAIR_12_PRODUCT_SIZE=1089 PRIMER_PAIR_13_PENALTY=7.221949 PRIMER_LEFT_13_PENALTY=2.598622 PRIMER_RIGHT_13_PENALTY=4.623327 PRIMER_LEFT_13_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_13_SEQUENCE=TTACACCCCAATCTTCATGTC PRIMER_LEFT_13=28,19 PRIMER_RIGHT_13=1113,21 PRIMER_LEFT_13_TM=58.401 PRIMER_RIGHT_13_TM=57.377 PRIMER_LEFT_13_GC_PERCENT=47.368 PRIMER_RIGHT_13_GC_PERCENT=42.857 PRIMER_LEFT_13_SELF_ANY=3.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=1.000000 PRIMER_LEFT_13_END_STABILITY=9.4000 PRIMER_RIGHT_13_END_STABILITY=6.3000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PAIR_13_PRODUCT_SIZE=1086 PRIMER_PAIR_14_PENALTY=7.346131 PRIMER_LEFT_14_PENALTY=1.335843 PRIMER_RIGHT_14_PENALTY=6.010288 PRIMER_LEFT_14_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_14_SEQUENCE=TGTTACACCCCAATCTTCATGTC PRIMER_LEFT_14=28,20 PRIMER_RIGHT_14=1115,23 PRIMER_LEFT_14_TM=61.336 PRIMER_RIGHT_14_TM=61.010 PRIMER_LEFT_14_GC_PERCENT=45.000 PRIMER_RIGHT_14_GC_PERCENT=43.478 PRIMER_LEFT_14_SELF_ANY=3.00 PRIMER_RIGHT_14_SELF_ANY=5.00 PRIMER_LEFT_14_SELF_END=3.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=2.000000 PRIMER_LEFT_14_END_STABILITY=10.0000 PRIMER_RIGHT_14_END_STABILITY=6.3000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PAIR_14_PRODUCT_SIZE=1088 PRIMER_PAIR_15_PENALTY=7.380130 PRIMER_LEFT_15_PENALTY=2.598622 PRIMER_RIGHT_15_PENALTY=4.781508 PRIMER_LEFT_15_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_15_SEQUENCE=TGTTACACCCCAATCTTCATGT PRIMER_LEFT_15=28,19 PRIMER_RIGHT_15=1115,22 PRIMER_LEFT_15_TM=58.401 PRIMER_RIGHT_15_TM=59.218 PRIMER_LEFT_15_GC_PERCENT=47.368 PRIMER_RIGHT_15_GC_PERCENT=40.909 PRIMER_LEFT_15_SELF_ANY=3.00 PRIMER_RIGHT_15_SELF_ANY=5.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=2.000000 PRIMER_LEFT_15_END_STABILITY=9.4000 PRIMER_RIGHT_15_END_STABILITY=6.6000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PAIR_15_PRODUCT_SIZE=1088 PRIMER_PAIR_16_PENALTY=7.527776 PRIMER_LEFT_16_PENALTY=2.598622 PRIMER_RIGHT_16_PENALTY=4.929154 PRIMER_LEFT_16_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_16_SEQUENCE=TACACCCCAATCTTCATGTCCA PRIMER_LEFT_16=28,19 PRIMER_RIGHT_16=1112,22 PRIMER_LEFT_16_TM=58.401 PRIMER_RIGHT_16_TM=62.429 PRIMER_LEFT_16_GC_PERCENT=47.368 PRIMER_RIGHT_16_GC_PERCENT=45.455 PRIMER_LEFT_16_SELF_ANY=3.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=2.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=0.500000 PRIMER_LEFT_16_END_STABILITY=9.4000 PRIMER_RIGHT_16_END_STABILITY=7.9000 PRIMER_PAIR_16_COMPL_ANY=6.00 PRIMER_PAIR_16_COMPL_END=3.00 PRIMER_PAIR_16_PRODUCT_SIZE=1085 PRIMER_PAIR_17_PENALTY=7.536840 PRIMER_LEFT_17_PENALTY=1.335843 PRIMER_RIGHT_17_PENALTY=6.200997 PRIMER_LEFT_17_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_17_SEQUENCE=GTTACACCCCAATCTTCATGTCC PRIMER_LEFT_17=28,20 PRIMER_RIGHT_17=1114,23 PRIMER_LEFT_17_TM=61.336 PRIMER_RIGHT_17_TM=61.701 PRIMER_LEFT_17_GC_PERCENT=45.000 PRIMER_RIGHT_17_GC_PERCENT=47.826 PRIMER_LEFT_17_SELF_ANY=3.00 PRIMER_RIGHT_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=1.500000 PRIMER_LEFT_17_END_STABILITY=10.0000 PRIMER_RIGHT_17_END_STABILITY=7.9000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PAIR_17_PRODUCT_SIZE=1087 PRIMER_PAIR_18_PENALTY=7.805664 PRIMER_LEFT_18_PENALTY=2.598622 PRIMER_RIGHT_18_PENALTY=5.207042 PRIMER_LEFT_18_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_18_SEQUENCE=GTTACACCCCAATCTTCATGTC PRIMER_LEFT_18=28,19 PRIMER_RIGHT_18=1114,22 PRIMER_LEFT_18_TM=58.401 PRIMER_RIGHT_18_TM=58.293 PRIMER_LEFT_18_GC_PERCENT=47.368 PRIMER_RIGHT_18_GC_PERCENT=45.455 PRIMER_LEFT_18_SELF_ANY=3.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=1.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=1.500000 PRIMER_LEFT_18_END_STABILITY=9.4000 PRIMER_RIGHT_18_END_STABILITY=6.3000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PAIR_18_PRODUCT_SIZE=1087 PRIMER_PAIR_19_PENALTY=7.831834 PRIMER_LEFT_19_PENALTY=2.598622 PRIMER_RIGHT_19_PENALTY=5.233212 PRIMER_LEFT_19_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_19_SEQUENCE=ACACCCCAATCTTCATGTCCAC PRIMER_LEFT_19=28,19 PRIMER_RIGHT_19=1111,22 PRIMER_LEFT_19_TM=58.401 PRIMER_RIGHT_19_TM=63.233 PRIMER_LEFT_19_GC_PERCENT=47.368 PRIMER_RIGHT_19_GC_PERCENT=50.000 PRIMER_LEFT_19_SELF_ANY=3.00 PRIMER_RIGHT_19_SELF_ANY=4.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=0.000000 PRIMER_LEFT_19_END_STABILITY=9.4000 PRIMER_RIGHT_19_END_STABILITY=7.9000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PAIR_19_PRODUCT_SIZE=1084 = SEQUENCE_ID=M67454/FAS P3_COMMENT=Human Fas antigen (fas) mRNA, complete cds. CDS_195..1202 SEQUENCE_START_CODON_POSITION=194 SEQUENCE_TEMPLATE=GACGCTTCTGGGGAGTGAGGGAAGCGGTTTACGAGTGACTTGGCTGGAGCCTCAGGGGCGGGCACTGGCACGGAACACACCCTGAGGCCAGCCCTGGCTGCCCAGGCGGAGCTGCCTCTTCTCCCGCGGGTTGGTGGACCCGCTCAGTACGGAGTTGGGGAAGCTCTTTCACTTCGGAGGATTGCTCAACAACCATGCTGGGCATCTGGACCCTCCTACCTCTGGTTCTTACGTCTGTTGCTAGATTATCGTCCAAAAGTGTTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCCTGCATCATGATGGCCAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGGGATGAACCAGACTGCGTGCCCTGCCAAGAAGGGAAGGAGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGACATGGCTTAGAAGTGGAAATAAACTGCACCCGGACCCAGAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGACCCTTGCACCAAATGTGAACATGGAATCATCAAGGAATGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGTGAAGAGAAAGGAAGTACAGAAAACATGCAGAAAGCACAGAAAGGAAAACCAAGGTTCTCATGAATCTCCAACCTTAAATCCTGAAACAGTGGCAATAAATTTATCTGATGTTGACTTGAGTAAATATATCACCACTATTGCTGGAGTCATGACACTAAGTCAAGTTAAAGGCTTTGTTCGAAAGAATGGTGTCAATGAAGCCAAAATAGATGAGATCAAGAATGACAATGTCCAAGACACAGCAGAACAGAAAGTTCAACTGCTTCGTAATTGGCATCAACTTCATGGAAAGAAAGAAGCGTATGACACATTGATTAAAGATCTCAAAAAAGCCAATCTTTGTACTCTTGCAGAGAAAATTCAGACTATCATCCTCAAGGACATTACTAGTGACTCAGAAAATTCAAACTTCAGAAATGAAATCCAAAGCTTGGTCTAGAGTGAAAAACAACAAATTCAGTTCTGAGTATATGCAATTAGTGTTTGAAAAGATTCTTAATAGCTGGCTGTAAATACTGCTTGGTTTTTTACTGGGTACATTTTATCATTTATTAGCGCTGAAGAGCCAACATATTTGTAGATTTTTAATATCTCATGATTCTGCCTCCAAGGATGTTTAAAATCTAGTTGGGAAAACAAACTTCATCAAGAGTAAATGCAGTGGCATGCTAAGTACCCAAATAGGAGTGTATGCAGAGGATGAAAGATTAAGATTATGCTCTGGCATCTAACATATGATTCTGTAGTATGAATGTAATCAGTGTATGTTAGTACAAATGTCTATCCACAGGCTAACCCCACTCTATGAATCAATAGAAGAAGCTATGACCTTTTGCTGAAATATCAGTTACTGAACAGGCAGGCCACTTTGCCTCTAAATTACCTCTGATAATTCTAGAGATTTTACCATATTTCTAAACTTTGTTTATAACTCTGAGAAGATCATATTTATGTAAAGTATATGTATTTGAGTGCAGAATTTAAATAAGGCTCTACCTCAAAGACCTTTGCACAGTTTATTGGTGTCATATTATACAATATTTCAATTGTGAATTCACATAGAAAACATTAAATTATAATGTTTGACTATTATATATGTGTATGCATTTTACTGGCTCAAAACTACCTACTTCTTTCTCAGGCATCAAAAGCATTTTGAGCAGGAGAGTATTACTAGAGCTTTGCCACCTCTCCATTTTTGCCTTGGTGCTCATCTTAATGGCCTAATGCACCCCCAAACATGGAAATATCACCAAAAAATACTTAATAGTCCACCAAAAGGCAAGACTGCCCTTAGAAATTCTAGCCTGGTTTGGAGATACTAACTGCTCTCAGAGAAAGTAGCTTTGTGACATGTCATGAACCCATGTTTGCAATCAAAGATGATAAAATAGATTCTTATTTTTCCCCCACCCCCGAAAATGTTCAATAATGTCCCATGTAAAACCTGCTACAAATGGCAGCTTATACATAGCAATGGTAAAATCATCATCTGGATTTAGGAATTGCTCTTGTCATACCCTCAAGTTTCTAAGATTTAAGATTCTCCTTACTACTATCCTACGTTTAAATATCTTTGAAAGTTTGTATTAAATGTGAATTTTAAGAAATAATATTTATATTTCTGTAAATGTAAACTGTGAAGATAGTTATAAACTGAAGCAGATACCTGGAACCACCTAAAGAACTTCCATTTATGGAGGATTTTTTTGCCCCTTGTGTTTGGAATTATAAAATATAGGTAAAAGTACGTAATTAAATAATGTTTTTG PRIMER_LEFT_EXPLAIN=considered 21749, would not amplify any of the ORF 18372, GC content failed 10, low tm 984, high tm 1002, long poly-x seq 44, high 3' stability 21, ok 1316 PRIMER_RIGHT_EXPLAIN=considered 21076, GC content failed 1260, low tm 8619, high tm 3776, long poly-x seq 112, high 3' stability 48, ok 7261 PRIMER_PAIR_EXPLAIN=considered 21, ok 21 PRIMER_STOP_CODON_POSITION=1199 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=124.131340 PRIMER_LEFT_0_PENALTY=121.524367 PRIMER_RIGHT_0_PENALTY=2.606973 PRIMER_LEFT_0_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_0_SEQUENCE=AGACCAAGCTTTGGATTTCA PRIMER_LEFT_0=200,19 PRIMER_RIGHT_0=1199,20 PRIMER_LEFT_0_TM=59.476 PRIMER_RIGHT_0_TM=57.393 PRIMER_LEFT_0_GC_PERCENT=63.158 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_POSITION_PENALTY=120.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=1000 PRIMER_PAIR_1_PENALTY=124.388123 PRIMER_LEFT_1_PENALTY=121.524367 PRIMER_RIGHT_1_PENALTY=2.863756 PRIMER_LEFT_1_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_1_SEQUENCE=AGACCAAGCTTTGGATTTCATT PRIMER_LEFT_1=200,19 PRIMER_RIGHT_1=1199,22 PRIMER_LEFT_1_TM=59.476 PRIMER_RIGHT_1_TM=59.136 PRIMER_LEFT_1_GC_PERCENT=63.158 PRIMER_RIGHT_1_GC_PERCENT=36.364 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_POSITION_PENALTY=120.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=1000 PRIMER_PAIR_2_PENALTY=124.725988 PRIMER_LEFT_2_PENALTY=121.524367 PRIMER_RIGHT_2_PENALTY=3.201621 PRIMER_LEFT_2_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_2_SEQUENCE=AGACCAAGCTTTGGATTTCAT PRIMER_LEFT_2=200,19 PRIMER_RIGHT_2=1199,21 PRIMER_LEFT_2_TM=59.476 PRIMER_RIGHT_2_TM=57.798 PRIMER_LEFT_2_GC_PERCENT=63.158 PRIMER_RIGHT_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_POSITION_PENALTY=120.000000 PRIMER_RIGHT_2_POSITION_PENALTY=0.000000 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=1000 PRIMER_PAIR_3_PENALTY=124.870439 PRIMER_LEFT_3_PENALTY=121.524367 PRIMER_RIGHT_3_PENALTY=3.346072 PRIMER_LEFT_3_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_3_SEQUENCE=AGACCAAGCTTTGGATTTCATTT PRIMER_LEFT_3=200,19 PRIMER_RIGHT_3=1199,23 PRIMER_LEFT_3_TM=59.476 PRIMER_RIGHT_3_TM=60.346 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=34.783 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_POSITION_PENALTY=120.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.000000 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=1000 PRIMER_PAIR_4_PENALTY=125.521988 PRIMER_LEFT_4_PENALTY=121.524367 PRIMER_RIGHT_4_PENALTY=3.997622 PRIMER_LEFT_4_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_4_SEQUENCE=TAGACCAAGCTTTGGATTTCA PRIMER_LEFT_4=200,19 PRIMER_RIGHT_4=1200,21 PRIMER_LEFT_4_TM=59.476 PRIMER_RIGHT_4_TM=57.502 PRIMER_LEFT_4_GC_PERCENT=63.158 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_POSITION_PENALTY=120.000000 PRIMER_RIGHT_4_POSITION_PENALTY=0.500000 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=7.3000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=1001 PRIMER_PAIR_5_PENALTY=125.689888 PRIMER_LEFT_5_PENALTY=123.082915 PRIMER_RIGHT_5_PENALTY=2.606973 PRIMER_LEFT_5_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_5_SEQUENCE=AGACCAAGCTTTGGATTTCA PRIMER_LEFT_5=200,20 PRIMER_RIGHT_5=1199,20 PRIMER_LEFT_5_TM=63.083 PRIMER_RIGHT_5_TM=57.393 PRIMER_LEFT_5_GC_PERCENT=65.000 PRIMER_RIGHT_5_GC_PERCENT=40.000 PRIMER_LEFT_5_SELF_ANY=3.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_POSITION_PENALTY=120.000000 PRIMER_RIGHT_5_POSITION_PENALTY=0.000000 PRIMER_LEFT_5_END_STABILITY=6.9000 PRIMER_RIGHT_5_END_STABILITY=7.3000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PAIR_5_PRODUCT_SIZE=1000 PRIMER_PAIR_6_PENALTY=125.848946 PRIMER_LEFT_6_PENALTY=121.524367 PRIMER_RIGHT_6_PENALTY=4.324579 PRIMER_LEFT_6_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_6_SEQUENCE=TAGACCAAGCTTTGGATTTCATT PRIMER_LEFT_6=200,19 PRIMER_RIGHT_6=1200,23 PRIMER_LEFT_6_TM=59.476 PRIMER_RIGHT_6_TM=59.175 PRIMER_LEFT_6_GC_PERCENT=63.158 PRIMER_RIGHT_6_GC_PERCENT=34.783 PRIMER_LEFT_6_SELF_ANY=3.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_POSITION_PENALTY=120.000000 PRIMER_RIGHT_6_POSITION_PENALTY=0.500000 PRIMER_LEFT_6_END_STABILITY=6.9000 PRIMER_RIGHT_6_END_STABILITY=6.9000 PRIMER_PAIR_6_COMPL_ANY=3.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PAIR_6_PRODUCT_SIZE=1001 PRIMER_PAIR_7_PENALTY=125.946671 PRIMER_LEFT_7_PENALTY=123.082915 PRIMER_RIGHT_7_PENALTY=2.863756 PRIMER_LEFT_7_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_7_SEQUENCE=AGACCAAGCTTTGGATTTCATT PRIMER_LEFT_7=200,20 PRIMER_RIGHT_7=1199,22 PRIMER_LEFT_7_TM=63.083 PRIMER_RIGHT_7_TM=59.136 PRIMER_LEFT_7_GC_PERCENT=65.000 PRIMER_RIGHT_7_GC_PERCENT=36.364 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_POSITION_PENALTY=120.000000 PRIMER_RIGHT_7_POSITION_PENALTY=0.000000 PRIMER_LEFT_7_END_STABILITY=6.9000 PRIMER_RIGHT_7_END_STABILITY=6.9000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PAIR_7_PRODUCT_SIZE=1000 PRIMER_PAIR_8_PENALTY=126.009466 PRIMER_LEFT_8_PENALTY=121.524367 PRIMER_RIGHT_8_PENALTY=4.485099 PRIMER_LEFT_8_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_8_SEQUENCE=CTAGACCAAGCTTTGGATTTCA PRIMER_LEFT_8=200,19 PRIMER_RIGHT_8=1201,22 PRIMER_LEFT_8_TM=59.476 PRIMER_RIGHT_8_TM=58.515 PRIMER_LEFT_8_GC_PERCENT=63.158 PRIMER_RIGHT_8_GC_PERCENT=40.909 PRIMER_LEFT_8_SELF_ANY=3.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_POSITION_PENALTY=120.000000 PRIMER_RIGHT_8_POSITION_PENALTY=1.000000 PRIMER_LEFT_8_END_STABILITY=6.9000 PRIMER_RIGHT_8_END_STABILITY=7.3000 PRIMER_PAIR_8_COMPL_ANY=3.00 PRIMER_PAIR_8_COMPL_END=0.00 PRIMER_PAIR_8_PRODUCT_SIZE=1002 PRIMER_PAIR_9_PENALTY=126.136752 PRIMER_LEFT_9_PENALTY=121.524367 PRIMER_RIGHT_9_PENALTY=4.612386 PRIMER_LEFT_9_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_9_SEQUENCE=TAGACCAAGCTTTGGATTTCAT PRIMER_LEFT_9=200,19 PRIMER_RIGHT_9=1200,22 PRIMER_LEFT_9_TM=59.476 PRIMER_RIGHT_9_TM=57.888 PRIMER_LEFT_9_GC_PERCENT=63.158 PRIMER_RIGHT_9_GC_PERCENT=36.364 PRIMER_LEFT_9_SELF_ANY=3.00 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_POSITION_PENALTY=120.000000 PRIMER_RIGHT_9_POSITION_PENALTY=0.500000 PRIMER_LEFT_9_END_STABILITY=6.9000 PRIMER_RIGHT_9_END_STABILITY=6.9000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PAIR_9_PRODUCT_SIZE=1001 PRIMER_PAIR_10_PENALTY=126.267350 PRIMER_LEFT_10_PENALTY=121.524367 PRIMER_RIGHT_10_PENALTY=4.742983 PRIMER_LEFT_10_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_10_SEQUENCE=TCTAGACCAAGCTTTGGATTTCA PRIMER_LEFT_10=200,19 PRIMER_RIGHT_10=1202,23 PRIMER_LEFT_10_TM=59.476 PRIMER_RIGHT_10_TM=60.243 PRIMER_LEFT_10_GC_PERCENT=63.158 PRIMER_RIGHT_10_GC_PERCENT=39.130 PRIMER_LEFT_10_SELF_ANY=3.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=3.00 PRIMER_LEFT_10_POSITION_PENALTY=120.000000 PRIMER_RIGHT_10_POSITION_PENALTY=1.500000 PRIMER_LEFT_10_END_STABILITY=6.9000 PRIMER_RIGHT_10_END_STABILITY=7.3000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=0.00 PRIMER_PAIR_10_PRODUCT_SIZE=1003 PRIMER_PAIR_11_PENALTY=126.284536 PRIMER_LEFT_11_PENALTY=123.082915 PRIMER_RIGHT_11_PENALTY=3.201621 PRIMER_LEFT_11_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_11_SEQUENCE=AGACCAAGCTTTGGATTTCAT PRIMER_LEFT_11=200,20 PRIMER_RIGHT_11=1199,21 PRIMER_LEFT_11_TM=63.083 PRIMER_RIGHT_11_TM=57.798 PRIMER_LEFT_11_GC_PERCENT=65.000 PRIMER_RIGHT_11_GC_PERCENT=38.095 PRIMER_LEFT_11_SELF_ANY=3.00 PRIMER_RIGHT_11_SELF_ANY=6.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=120.000000 PRIMER_RIGHT_11_POSITION_PENALTY=0.000000 PRIMER_LEFT_11_END_STABILITY=6.9000 PRIMER_RIGHT_11_END_STABILITY=6.9000 PRIMER_PAIR_11_COMPL_ANY=3.00 PRIMER_PAIR_11_COMPL_END=0.00 PRIMER_PAIR_11_PRODUCT_SIZE=1000 PRIMER_PAIR_12_PENALTY=126.368366 PRIMER_LEFT_12_PENALTY=121.524367 PRIMER_RIGHT_12_PENALTY=4.843999 PRIMER_LEFT_12_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_12_SEQUENCE=TAGACCAAGCTTTGGATTTCATTT PRIMER_LEFT_12=200,19 PRIMER_RIGHT_12=1200,24 PRIMER_LEFT_12_TM=59.476 PRIMER_RIGHT_12_TM=60.344 PRIMER_LEFT_12_GC_PERCENT=63.158 PRIMER_RIGHT_12_GC_PERCENT=33.333 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=6.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_POSITION_PENALTY=120.000000 PRIMER_RIGHT_12_POSITION_PENALTY=0.500000 PRIMER_LEFT_12_END_STABILITY=6.9000 PRIMER_RIGHT_12_END_STABILITY=7.2000 PRIMER_PAIR_12_COMPL_ANY=3.00 PRIMER_PAIR_12_COMPL_END=0.00 PRIMER_PAIR_12_PRODUCT_SIZE=1001 PRIMER_PAIR_13_PENALTY=126.428987 PRIMER_LEFT_13_PENALTY=123.082915 PRIMER_RIGHT_13_PENALTY=3.346072 PRIMER_LEFT_13_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_13_SEQUENCE=AGACCAAGCTTTGGATTTCATTT PRIMER_LEFT_13=200,20 PRIMER_RIGHT_13=1199,23 PRIMER_LEFT_13_TM=63.083 PRIMER_RIGHT_13_TM=60.346 PRIMER_LEFT_13_GC_PERCENT=65.000 PRIMER_RIGHT_13_GC_PERCENT=34.783 PRIMER_LEFT_13_SELF_ANY=3.00 PRIMER_RIGHT_13_SELF_ANY=6.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=0.00 PRIMER_LEFT_13_POSITION_PENALTY=120.000000 PRIMER_RIGHT_13_POSITION_PENALTY=0.000000 PRIMER_LEFT_13_END_STABILITY=6.9000 PRIMER_RIGHT_13_END_STABILITY=7.2000 PRIMER_PAIR_13_COMPL_ANY=3.00 PRIMER_PAIR_13_COMPL_END=0.00 PRIMER_PAIR_13_PRODUCT_SIZE=1000 PRIMER_PAIR_14_PENALTY=126.550645 PRIMER_LEFT_14_PENALTY=121.524367 PRIMER_RIGHT_14_PENALTY=5.026278 PRIMER_LEFT_14_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_14_SEQUENCE=CTAGACCAAGCTTTGGATTTCATT PRIMER_LEFT_14=200,19 PRIMER_RIGHT_14=1201,24 PRIMER_LEFT_14_TM=59.476 PRIMER_RIGHT_14_TM=60.026 PRIMER_LEFT_14_GC_PERCENT=63.158 PRIMER_RIGHT_14_GC_PERCENT=37.500 PRIMER_LEFT_14_SELF_ANY=3.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_POSITION_PENALTY=120.000000 PRIMER_RIGHT_14_POSITION_PENALTY=1.000000 PRIMER_LEFT_14_END_STABILITY=6.9000 PRIMER_RIGHT_14_END_STABILITY=6.9000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=0.00 PRIMER_PAIR_14_PRODUCT_SIZE=1002 PRIMER_PAIR_15_PENALTY=126.689801 PRIMER_LEFT_15_PENALTY=121.524367 PRIMER_RIGHT_15_PENALTY=5.165434 PRIMER_LEFT_15_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_15_SEQUENCE=CTAGACCAAGCTTTGGATTTCAT PRIMER_LEFT_15=200,19 PRIMER_RIGHT_15=1201,23 PRIMER_LEFT_15_TM=59.476 PRIMER_RIGHT_15_TM=58.835 PRIMER_LEFT_15_GC_PERCENT=63.158 PRIMER_RIGHT_15_GC_PERCENT=39.130 PRIMER_LEFT_15_SELF_ANY=3.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=120.000000 PRIMER_RIGHT_15_POSITION_PENALTY=1.000000 PRIMER_LEFT_15_END_STABILITY=6.9000 PRIMER_RIGHT_15_END_STABILITY=6.9000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=1002 PRIMER_PAIR_16_PENALTY=127.080537 PRIMER_LEFT_16_PENALTY=123.082915 PRIMER_RIGHT_16_PENALTY=3.997622 PRIMER_LEFT_16_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_16_SEQUENCE=TAGACCAAGCTTTGGATTTCA PRIMER_LEFT_16=200,20 PRIMER_RIGHT_16=1200,21 PRIMER_LEFT_16_TM=63.083 PRIMER_RIGHT_16_TM=57.502 PRIMER_LEFT_16_GC_PERCENT=65.000 PRIMER_RIGHT_16_GC_PERCENT=38.095 PRIMER_LEFT_16_SELF_ANY=3.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=3.00 PRIMER_LEFT_16_POSITION_PENALTY=120.000000 PRIMER_RIGHT_16_POSITION_PENALTY=0.500000 PRIMER_LEFT_16_END_STABILITY=6.9000 PRIMER_RIGHT_16_END_STABILITY=7.3000 PRIMER_PAIR_16_COMPL_ANY=3.00 PRIMER_PAIR_16_COMPL_END=0.00 PRIMER_PAIR_16_PRODUCT_SIZE=1001 PRIMER_PAIR_17_PENALTY=127.389795 PRIMER_LEFT_17_PENALTY=121.524367 PRIMER_RIGHT_17_PENALTY=5.865428 PRIMER_LEFT_17_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_17_SEQUENCE=TCTAGACCAAGCTTTGGATTTC PRIMER_LEFT_17=200,19 PRIMER_RIGHT_17=1202,22 PRIMER_LEFT_17_TM=59.476 PRIMER_RIGHT_17_TM=57.635 PRIMER_LEFT_17_GC_PERCENT=63.158 PRIMER_RIGHT_17_GC_PERCENT=40.909 PRIMER_LEFT_17_SELF_ANY=3.00 PRIMER_RIGHT_17_SELF_ANY=6.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=120.000000 PRIMER_RIGHT_17_POSITION_PENALTY=1.500000 PRIMER_LEFT_17_END_STABILITY=6.9000 PRIMER_RIGHT_17_END_STABILITY=6.9000 PRIMER_PAIR_17_COMPL_ANY=4.00 PRIMER_PAIR_17_COMPL_END=0.00 PRIMER_PAIR_17_PRODUCT_SIZE=1003 PRIMER_PAIR_18_PENALTY=127.407494 PRIMER_LEFT_18_PENALTY=123.082915 PRIMER_RIGHT_18_PENALTY=4.324579 PRIMER_LEFT_18_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_18_SEQUENCE=TAGACCAAGCTTTGGATTTCATT PRIMER_LEFT_18=200,20 PRIMER_RIGHT_18=1200,23 PRIMER_LEFT_18_TM=63.083 PRIMER_RIGHT_18_TM=59.175 PRIMER_LEFT_18_GC_PERCENT=65.000 PRIMER_RIGHT_18_GC_PERCENT=34.783 PRIMER_LEFT_18_SELF_ANY=3.00 PRIMER_RIGHT_18_SELF_ANY=6.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=1.00 PRIMER_LEFT_18_POSITION_PENALTY=120.000000 PRIMER_RIGHT_18_POSITION_PENALTY=0.500000 PRIMER_LEFT_18_END_STABILITY=6.9000 PRIMER_RIGHT_18_END_STABILITY=6.9000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PAIR_18_PRODUCT_SIZE=1001 PRIMER_PAIR_19_PENALTY=127.435780 PRIMER_LEFT_19_PENALTY=121.524367 PRIMER_RIGHT_19_PENALTY=5.911413 PRIMER_LEFT_19_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_19_SEQUENCE=AGACCAAGCTTTGGATTTCATTTC PRIMER_LEFT_19=200,19 PRIMER_RIGHT_19=1199,24 PRIMER_LEFT_19_TM=59.476 PRIMER_RIGHT_19_TM=61.911 PRIMER_LEFT_19_GC_PERCENT=63.158 PRIMER_RIGHT_19_GC_PERCENT=37.500 PRIMER_LEFT_19_SELF_ANY=3.00 PRIMER_RIGHT_19_SELF_ANY=6.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=1.00 PRIMER_LEFT_19_POSITION_PENALTY=120.000000 PRIMER_RIGHT_19_POSITION_PENALTY=0.000000 PRIMER_LEFT_19_END_STABILITY=6.9000 PRIMER_RIGHT_19_END_STABILITY=6.9000 PRIMER_PAIR_19_COMPL_ANY=3.00 PRIMER_PAIR_19_COMPL_END=0.00 PRIMER_PAIR_19_PRODUCT_SIZE=1000 = SEQUENCE_ID=U33760/SKP1 P3_COMMENT=Human cyclin A/CDK2-associated p19 (Skp1) mRNA, complete cds. CDS_94..585 SEQUENCE_START_CODON_POSITION=93 SEQUENCE_TEMPLATE=AGGATCCTCGAGGCCACGAAGGCCCTCTCCCTTCGCAAACGCCTCCCGGCTCTCGTAAGCCTCCCGCCGGCCGTCTCCTTAACACCGAACACCATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAGAGATATTTGAAGTTGATGTGGAAATTGCCAAACAATCTGTAACTATTAAGACCATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACCCAGTTCCTCTACCAAATGTGAATGCAGCAATATTAAAAAAGGTCATTCAGTGGTGCACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAGAACAAAGAAAAGCGGACAGATGATATCCCTGTTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTTTGCTTGATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTGAGGAGATTCGCAAGACCTTCAATATCAAAAATGACTTTACTGAAGAGGAGGAAGCCCAGGTACGCAAAGAGAACCAGTGGTGTGAAGAGAAGTGAAATGTTGTGCCTGACACTGTAACACTGTAAGGAT PRIMER_LEFT_EXPLAIN=considered 4706, would not amplify any of the ORF 3123, GC content failed 5, low tm 396, high tm 523, high end compl 7, long poly-x seq 20, high 3' stability 13, ok 619 PRIMER_RIGHT_EXPLAIN=considered 4645, GC content failed 11, low tm 1320, high tm 1167, high end compl 10, long poly-x seq 22, high 3' stability 16, ok 2099 PRIMER_PAIR_EXPLAIN=considered 21, ok 21 PRIMER_STOP_CODON_POSITION=582 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=12.352199 PRIMER_LEFT_0_PENALTY=9.051374 PRIMER_RIGHT_0_PENALTY=3.300825 PRIMER_LEFT_0_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_0_SEQUENCE=ACTTCTCTTCACACCACTGGTTC PRIMER_LEFT_0=93,26 PRIMER_RIGHT_0=582,23 PRIMER_LEFT_0_TM=63.051 PRIMER_RIGHT_0_TM=59.699 PRIMER_LEFT_0_GC_PERCENT=38.462 PRIMER_RIGHT_0_GC_PERCENT=47.826 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=490 PRIMER_PAIR_1_PENALTY=13.663544 PRIMER_LEFT_1_PENALTY=9.051374 PRIMER_RIGHT_1_PENALTY=4.612170 PRIMER_LEFT_1_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_1_SEQUENCE=ACTTCTCTTCACACCACTGGTTCT PRIMER_LEFT_1=93,26 PRIMER_RIGHT_1=582,24 PRIMER_LEFT_1_TM=63.051 PRIMER_RIGHT_1_TM=60.612 PRIMER_LEFT_1_GC_PERCENT=38.462 PRIMER_RIGHT_1_GC_PERCENT=45.833 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=490 PRIMER_PAIR_2_PENALTY=14.562250 PRIMER_LEFT_2_PENALTY=9.051374 PRIMER_RIGHT_2_PENALTY=5.510876 PRIMER_LEFT_2_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_2_SEQUENCE=TTCACTTCTCTTCACACCACTG PRIMER_LEFT_2=93,26 PRIMER_RIGHT_2=585,22 PRIMER_LEFT_2_TM=63.051 PRIMER_RIGHT_2_TM=57.989 PRIMER_LEFT_2_GC_PERCENT=38.462 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=1.500000 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=493 PRIMER_PAIR_3_PENALTY=14.622091 PRIMER_LEFT_3_PENALTY=9.051374 PRIMER_RIGHT_3_PENALTY=5.570716 PRIMER_LEFT_3_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_3_SEQUENCE=TTTCACTTCTCTTCACACCACTG PRIMER_LEFT_3=93,26 PRIMER_RIGHT_3=586,23 PRIMER_LEFT_3_TM=63.051 PRIMER_RIGHT_3_TM=59.429 PRIMER_LEFT_3_GC_PERCENT=38.462 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=2.000000 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=494 PRIMER_PAIR_4_PENALTY=15.830970 PRIMER_LEFT_4_PENALTY=9.051374 PRIMER_RIGHT_4_PENALTY=6.779595 PRIMER_LEFT_4_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_4_SEQUENCE=ATTTCACTTCTCTTCACACCACTG PRIMER_LEFT_4=93,26 PRIMER_RIGHT_4=587,24 PRIMER_LEFT_4_TM=63.051 PRIMER_RIGHT_4_TM=59.720 PRIMER_LEFT_4_GC_PERCENT=38.462 PRIMER_RIGHT_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=2.500000 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=495 PRIMER_PAIR_5_PENALTY=15.917260 PRIMER_LEFT_5_PENALTY=9.051374 PRIMER_RIGHT_5_PENALTY=6.865886 PRIMER_LEFT_5_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_5_SEQUENCE=CACTTCTCTTCACACCACTGGTTC PRIMER_LEFT_5=93,26 PRIMER_RIGHT_5=583,24 PRIMER_LEFT_5_TM=63.051 PRIMER_RIGHT_5_TM=62.366 PRIMER_LEFT_5_GC_PERCENT=38.462 PRIMER_RIGHT_5_GC_PERCENT=50.000 PRIMER_LEFT_5_SELF_ANY=8.00 PRIMER_RIGHT_5_SELF_ANY=7.00 PRIMER_LEFT_5_SELF_END=2.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=0.500000 PRIMER_LEFT_5_END_STABILITY=6.4000 PRIMER_RIGHT_5_END_STABILITY=7.9000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PAIR_5_PRODUCT_SIZE=491 PRIMER_PAIR_6_PENALTY=16.195538 PRIMER_LEFT_6_PENALTY=9.051374 PRIMER_RIGHT_6_PENALTY=7.144164 PRIMER_LEFT_6_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_6_SEQUENCE=CATTTCACTTCTCTTCACACCA PRIMER_LEFT_6=93,26 PRIMER_RIGHT_6=588,22 PRIMER_LEFT_6_TM=63.051 PRIMER_RIGHT_6_TM=57.856 PRIMER_LEFT_6_GC_PERCENT=38.462 PRIMER_RIGHT_6_GC_PERCENT=40.909 PRIMER_LEFT_6_SELF_ANY=8.00 PRIMER_RIGHT_6_SELF_ANY=2.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=3.000000 PRIMER_LEFT_6_END_STABILITY=6.4000 PRIMER_RIGHT_6_END_STABILITY=8.2000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PAIR_6_PRODUCT_SIZE=496 PRIMER_PAIR_7_PENALTY=16.293810 PRIMER_LEFT_7_PENALTY=9.051374 PRIMER_RIGHT_7_PENALTY=7.242436 PRIMER_LEFT_7_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_7_SEQUENCE=CATTTCACTTCTCTTCACACCAC PRIMER_LEFT_7=93,26 PRIMER_RIGHT_7=588,23 PRIMER_LEFT_7_TM=63.051 PRIMER_RIGHT_7_TM=58.758 PRIMER_LEFT_7_GC_PERCENT=38.462 PRIMER_RIGHT_7_GC_PERCENT=43.478 PRIMER_LEFT_7_SELF_ANY=8.00 PRIMER_RIGHT_7_SELF_ANY=2.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=3.000000 PRIMER_LEFT_7_END_STABILITY=6.4000 PRIMER_RIGHT_7_END_STABILITY=7.6000 PRIMER_PAIR_7_COMPL_ANY=5.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PAIR_7_PRODUCT_SIZE=496 PRIMER_PAIR_8_PENALTY=16.329628 PRIMER_LEFT_8_PENALTY=9.051374 PRIMER_RIGHT_8_PENALTY=7.278253 PRIMER_LEFT_8_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_8_SEQUENCE=ACTTCTCTTCACACCACTGGTTCTC PRIMER_LEFT_8=93,26 PRIMER_RIGHT_8=582,25 PRIMER_LEFT_8_TM=63.051 PRIMER_RIGHT_8_TM=62.278 PRIMER_LEFT_8_GC_PERCENT=38.462 PRIMER_RIGHT_8_GC_PERCENT=48.000 PRIMER_LEFT_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_ANY=7.00 PRIMER_LEFT_8_SELF_END=2.00 PRIMER_RIGHT_8_SELF_END=1.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=0.000000 PRIMER_LEFT_8_END_STABILITY=6.4000 PRIMER_RIGHT_8_END_STABILITY=6.7000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PAIR_8_PRODUCT_SIZE=490 PRIMER_PAIR_9_PENALTY=16.330970 PRIMER_LEFT_9_PENALTY=9.051374 PRIMER_RIGHT_9_PENALTY=7.279595 PRIMER_LEFT_9_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_9_SEQUENCE=CATTTCACTTCTCTTCACACCACT PRIMER_LEFT_9=93,26 PRIMER_RIGHT_9=588,24 PRIMER_LEFT_9_TM=63.051 PRIMER_RIGHT_9_TM=59.720 PRIMER_LEFT_9_GC_PERCENT=38.462 PRIMER_RIGHT_9_GC_PERCENT=41.667 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=2.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=1.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=3.000000 PRIMER_LEFT_9_END_STABILITY=6.4000 PRIMER_RIGHT_9_END_STABILITY=7.9000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=496 PRIMER_PAIR_10_PENALTY=16.793810 PRIMER_LEFT_10_PENALTY=9.051374 PRIMER_RIGHT_10_PENALTY=7.742436 PRIMER_LEFT_10_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_10_SEQUENCE=ACATTTCACTTCTCTTCACACCA PRIMER_LEFT_10=93,26 PRIMER_RIGHT_10=589,23 PRIMER_LEFT_10_TM=63.051 PRIMER_RIGHT_10_TM=58.758 PRIMER_LEFT_10_GC_PERCENT=38.462 PRIMER_RIGHT_10_GC_PERCENT=39.130 PRIMER_LEFT_10_SELF_ANY=8.00 PRIMER_RIGHT_10_SELF_ANY=2.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=3.500000 PRIMER_LEFT_10_END_STABILITY=6.4000 PRIMER_RIGHT_10_END_STABILITY=8.2000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=0.00 PRIMER_PAIR_10_PRODUCT_SIZE=497 PRIMER_PAIR_11_PENALTY=16.959781 PRIMER_LEFT_11_PENALTY=9.051374 PRIMER_RIGHT_11_PENALTY=7.908407 PRIMER_LEFT_11_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_11_SEQUENCE=ACATTTCACTTCTCTTCACACCAC PRIMER_LEFT_11=93,26 PRIMER_RIGHT_11=589,24 PRIMER_LEFT_11_TM=63.051 PRIMER_RIGHT_11_TM=59.592 PRIMER_LEFT_11_GC_PERCENT=38.462 PRIMER_RIGHT_11_GC_PERCENT=41.667 PRIMER_LEFT_11_SELF_ANY=8.00 PRIMER_RIGHT_11_SELF_ANY=2.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=3.500000 PRIMER_LEFT_11_END_STABILITY=6.4000 PRIMER_RIGHT_11_END_STABILITY=7.6000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=0.00 PRIMER_PAIR_11_PRODUCT_SIZE=497 PRIMER_PAIR_12_PENALTY=17.126181 PRIMER_LEFT_12_PENALTY=9.051374 PRIMER_RIGHT_12_PENALTY=8.074807 PRIMER_LEFT_12_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_12_SEQUENCE=AACATTTCACTTCTCTTCACACCA PRIMER_LEFT_12=93,26 PRIMER_RIGHT_12=590,24 PRIMER_LEFT_12_TM=63.051 PRIMER_RIGHT_12_TM=60.075 PRIMER_LEFT_12_GC_PERCENT=38.462 PRIMER_RIGHT_12_GC_PERCENT=37.500 PRIMER_LEFT_12_SELF_ANY=8.00 PRIMER_RIGHT_12_SELF_ANY=2.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=4.000000 PRIMER_LEFT_12_END_STABILITY=6.4000 PRIMER_RIGHT_12_END_STABILITY=8.2000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PAIR_12_PRODUCT_SIZE=498 PRIMER_PAIR_13_PENALTY=17.549645 PRIMER_LEFT_13_PENALTY=9.051374 PRIMER_RIGHT_13_PENALTY=8.498271 PRIMER_LEFT_13_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_13_SEQUENCE=ATTTCACTTCTCTTCACACCACT PRIMER_LEFT_13=93,26 PRIMER_RIGHT_13=587,23 PRIMER_LEFT_13_TM=63.051 PRIMER_RIGHT_13_TM=57.002 PRIMER_LEFT_13_GC_PERCENT=38.462 PRIMER_RIGHT_13_GC_PERCENT=39.130 PRIMER_LEFT_13_SELF_ANY=8.00 PRIMER_RIGHT_13_SELF_ANY=2.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=2.500000 PRIMER_LEFT_13_END_STABILITY=6.4000 PRIMER_RIGHT_13_END_STABILITY=7.9000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PAIR_13_PRODUCT_SIZE=495 PRIMER_PAIR_14_PENALTY=17.626181 PRIMER_LEFT_14_PENALTY=9.051374 PRIMER_RIGHT_14_PENALTY=8.574807 PRIMER_LEFT_14_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_14_SEQUENCE=CAACATTTCACTTCTCTTCACACC PRIMER_LEFT_14=93,26 PRIMER_RIGHT_14=591,24 PRIMER_LEFT_14_TM=63.051 PRIMER_RIGHT_14_TM=60.075 PRIMER_LEFT_14_GC_PERCENT=38.462 PRIMER_RIGHT_14_GC_PERCENT=41.667 PRIMER_LEFT_14_SELF_ANY=8.00 PRIMER_RIGHT_14_SELF_ANY=2.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=4.500000 PRIMER_LEFT_14_END_STABILITY=6.4000 PRIMER_RIGHT_14_END_STABILITY=7.6000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=1.00 PRIMER_PAIR_14_PRODUCT_SIZE=499 PRIMER_PAIR_15_PENALTY=17.690498 PRIMER_LEFT_15_PENALTY=9.051374 PRIMER_RIGHT_15_PENALTY=8.639124 PRIMER_LEFT_15_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_15_SEQUENCE=CACTTCTCTTCACACCACTGGTTCT PRIMER_LEFT_15=93,26 PRIMER_RIGHT_15=583,25 PRIMER_LEFT_15_TM=63.051 PRIMER_RIGHT_15_TM=63.139 PRIMER_LEFT_15_GC_PERCENT=38.462 PRIMER_RIGHT_15_GC_PERCENT=48.000 PRIMER_LEFT_15_SELF_ANY=8.00 PRIMER_RIGHT_15_SELF_ANY=7.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=3.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=0.500000 PRIMER_LEFT_15_END_STABILITY=6.4000 PRIMER_RIGHT_15_END_STABILITY=6.4000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PAIR_15_PRODUCT_SIZE=491 PRIMER_PAIR_16_PENALTY=18.035208 PRIMER_LEFT_16_PENALTY=9.051374 PRIMER_RIGHT_16_PENALTY=8.983834 PRIMER_LEFT_16_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_16_SEQUENCE=ACATTTCACTTCTCTTCACACCACT PRIMER_LEFT_16=93,26 PRIMER_RIGHT_16=589,25 PRIMER_LEFT_16_TM=63.051 PRIMER_RIGHT_16_TM=60.484 PRIMER_LEFT_16_GC_PERCENT=38.462 PRIMER_RIGHT_16_GC_PERCENT=40.000 PRIMER_LEFT_16_SELF_ANY=8.00 PRIMER_RIGHT_16_SELF_ANY=2.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=1.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=3.500000 PRIMER_LEFT_16_END_STABILITY=6.4000 PRIMER_RIGHT_16_END_STABILITY=7.9000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PAIR_16_PRODUCT_SIZE=497 PRIMER_PAIR_17_PENALTY=18.073566 PRIMER_LEFT_17_PENALTY=9.051374 PRIMER_RIGHT_17_PENALTY=9.022192 PRIMER_LEFT_17_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_17_SEQUENCE=ACTTCTCTTCACACCACTGGTTCTCT PRIMER_LEFT_17=93,26 PRIMER_RIGHT_17=582,26 PRIMER_LEFT_17_TM=63.051 PRIMER_RIGHT_17_TM=63.022 PRIMER_LEFT_17_GC_PERCENT=38.462 PRIMER_RIGHT_17_GC_PERCENT=46.154 PRIMER_LEFT_17_SELF_ANY=8.00 PRIMER_RIGHT_17_SELF_ANY=7.00 PRIMER_LEFT_17_SELF_END=2.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=0.000000 PRIMER_LEFT_17_END_STABILITY=6.4000 PRIMER_RIGHT_17_END_STABILITY=6.4000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=3.00 PRIMER_PAIR_17_PRODUCT_SIZE=490 PRIMER_PAIR_18_PENALTY=18.636182 PRIMER_LEFT_18_PENALTY=9.051374 PRIMER_RIGHT_18_PENALTY=9.584808 PRIMER_LEFT_18_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_18_SEQUENCE=GCACAACATTTCACTTCTCTTCA PRIMER_LEFT_18=93,26 PRIMER_RIGHT_18=594,23 PRIMER_LEFT_18_TM=63.051 PRIMER_RIGHT_18_TM=59.415 PRIMER_LEFT_18_GC_PERCENT=38.462 PRIMER_RIGHT_18_GC_PERCENT=39.130 PRIMER_LEFT_18_SELF_ANY=8.00 PRIMER_RIGHT_18_SELF_ANY=2.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=1.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=6.000000 PRIMER_LEFT_18_END_STABILITY=6.4000 PRIMER_RIGHT_18_END_STABILITY=7.0000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PAIR_18_PRODUCT_SIZE=502 PRIMER_PAIR_19_PENALTY=18.666052 PRIMER_LEFT_19_PENALTY=9.051374 PRIMER_RIGHT_19_PENALTY=9.614678 PRIMER_LEFT_19_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_19_SEQUENCE=AACATTTCACTTCTCTTCACACC PRIMER_LEFT_19=93,26 PRIMER_RIGHT_19=590,23 PRIMER_LEFT_19_TM=63.051 PRIMER_RIGHT_19_TM=57.385 PRIMER_LEFT_19_GC_PERCENT=38.462 PRIMER_RIGHT_19_GC_PERCENT=39.130 PRIMER_LEFT_19_SELF_ANY=8.00 PRIMER_RIGHT_19_SELF_ANY=2.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=4.000000 PRIMER_LEFT_19_END_STABILITY=6.4000 PRIMER_RIGHT_19_END_STABILITY=7.6000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=1.00 PRIMER_PAIR_19_PRODUCT_SIZE=498 = SEQUENCE_ID=X80343/P35 P3_COMMENT=H.sapiens p35 mRNA for regulatory subunit of cdk5 kinase. CDS_98..1021 SEQUENCE_START_CODON_POSITION=97 SEQUENCE_TEMPLATE=AAACTCAGAATTTTCGCGGGCTCGGTGAGCGGTTTTATCCCTCCGGCCGGCAGGCTGGGCGCAGGGGGCGAGCCCCCGCCCGGCGCGCAGCAGCACCATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGCTGTTTGAGGATGGCGCGGCCACCGTGGGCCACTATACGGCCGTACAGAACAGCAAGAACGCCAAGGACAAGAACCTGAAGCGCCACTCCATCATCTCCGTGCTGCCTTGGAAGAGAATCGTGGCCGTGTCGGCCAAGAAGAAGAACTCCAAGAAGGTGCAGCCTAACAGCAGCTACCAGAACAACATCACGCACCTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCAACCTGTCCACATTCGCCCAGCCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGGGTTCCCAGACCGGGGGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAACGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGTTGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACACAGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTAGGCCTGGATCGGTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATTTTTAAGAATTTATTATTAAATCAGTTTTGTGTACAG PRIMER_LEFT_EXPLAIN=considered 8968, would not amplify any of the ORF 6063, GC content failed 212, low tm 125, high tm 1996, high 3' stability 33, ok 539 PRIMER_RIGHT_EXPLAIN=considered 8912, GC content failed 682, low tm 430, high tm 6084, high end compl 14, high 3' stability 37, ok 1665 PRIMER_PAIR_EXPLAIN=considered 26, high end compl 4, ok 22 PRIMER_STOP_CODON_POSITION=1018 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=127.466882 PRIMER_LEFT_0_PENALTY=122.322207 PRIMER_RIGHT_0_PENALTY=5.144675 PRIMER_LEFT_0_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_0_SEQUENCE=TACAGTGCTCACCGATCCAG PRIMER_LEFT_0=103,19 PRIMER_RIGHT_0=1028,20 PRIMER_LEFT_0_TM=61.322 PRIMER_RIGHT_0_TM=59.855 PRIMER_LEFT_0_GC_PERCENT=63.158 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_POSITION_PENALTY=120.000000 PRIMER_RIGHT_0_POSITION_PENALTY=5.000000 PRIMER_LEFT_0_END_STABILITY=6.1000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=926 PRIMER_PAIR_1_PENALTY=127.966882 PRIMER_LEFT_1_PENALTY=122.322207 PRIMER_RIGHT_1_PENALTY=5.644675 PRIMER_LEFT_1_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_1_SEQUENCE=CTACAGTGCTCACCGATCCA PRIMER_LEFT_1=103,19 PRIMER_RIGHT_1=1029,20 PRIMER_LEFT_1_TM=61.322 PRIMER_RIGHT_1_TM=59.855 PRIMER_LEFT_1_GC_PERCENT=63.158 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_POSITION_PENALTY=120.000000 PRIMER_RIGHT_1_POSITION_PENALTY=5.500000 PRIMER_LEFT_1_END_STABILITY=6.1000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=927 PRIMER_PAIR_2_PENALTY=127.985635 PRIMER_LEFT_2_PENALTY=122.322207 PRIMER_RIGHT_2_PENALTY=5.663428 PRIMER_LEFT_2_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_2_SEQUENCE=ACAGTGCTCACCGATCCAG PRIMER_LEFT_2=103,19 PRIMER_RIGHT_2=1027,19 PRIMER_LEFT_2_TM=61.322 PRIMER_RIGHT_2_TM=59.837 PRIMER_LEFT_2_GC_PERCENT=63.158 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_POSITION_PENALTY=120.000000 PRIMER_RIGHT_2_POSITION_PENALTY=4.500000 PRIMER_LEFT_2_END_STABILITY=6.1000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=925 PRIMER_PAIR_3_PENALTY=129.155466 PRIMER_LEFT_3_PENALTY=122.322207 PRIMER_RIGHT_3_PENALTY=6.833259 PRIMER_LEFT_3_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_3_SEQUENCE=GCTACAGTGCTCACCGATCC PRIMER_LEFT_3=103,19 PRIMER_RIGHT_3=1030,20 PRIMER_LEFT_3_TM=61.322 PRIMER_RIGHT_3_TM=60.833 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_POSITION_PENALTY=120.000000 PRIMER_RIGHT_3_POSITION_PENALTY=6.000000 PRIMER_LEFT_3_END_STABILITY=6.1000 PRIMER_RIGHT_3_END_STABILITY=7.8000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=928 PRIMER_PAIR_4_PENALTY=129.424588 PRIMER_LEFT_4_PENALTY=122.322207 PRIMER_RIGHT_4_PENALTY=7.102380 PRIMER_LEFT_4_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_4_SEQUENCE=AGGCTACAGTGCTCACCGAT PRIMER_LEFT_4=103,19 PRIMER_RIGHT_4=1032,20 PRIMER_LEFT_4_TM=61.322 PRIMER_RIGHT_4_TM=59.898 PRIMER_LEFT_4_GC_PERCENT=63.158 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_POSITION_PENALTY=120.000000 PRIMER_RIGHT_4_POSITION_PENALTY=7.000000 PRIMER_LEFT_4_END_STABILITY=6.1000 PRIMER_RIGHT_4_END_STABILITY=9.8000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=930 PRIMER_PAIR_5_PENALTY=129.580280 PRIMER_LEFT_5_PENALTY=122.322207 PRIMER_RIGHT_5_PENALTY=7.258073 PRIMER_LEFT_5_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_5_SEQUENCE=TACAGTGCTCACCGATCCA PRIMER_LEFT_5=103,19 PRIMER_RIGHT_5=1028,19 PRIMER_LEFT_5_TM=61.322 PRIMER_RIGHT_5_TM=58.742 PRIMER_LEFT_5_GC_PERCENT=63.158 PRIMER_RIGHT_5_GC_PERCENT=52.632 PRIMER_LEFT_5_SELF_ANY=3.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_POSITION_PENALTY=120.000000 PRIMER_RIGHT_5_POSITION_PENALTY=5.000000 PRIMER_LEFT_5_END_STABILITY=6.1000 PRIMER_RIGHT_5_END_STABILITY=8.1000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PAIR_5_PRODUCT_SIZE=926 PRIMER_PAIR_6_PENALTY=129.681344 PRIMER_LEFT_6_PENALTY=122.322207 PRIMER_RIGHT_6_PENALTY=7.359137 PRIMER_LEFT_6_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_6_SEQUENCE=CTACAGTGCTCACCGATCCAG PRIMER_LEFT_6=103,19 PRIMER_RIGHT_6=1029,21 PRIMER_LEFT_6_TM=61.322 PRIMER_RIGHT_6_TM=60.859 PRIMER_LEFT_6_GC_PERCENT=63.158 PRIMER_RIGHT_6_GC_PERCENT=57.143 PRIMER_LEFT_6_SELF_ANY=3.00 PRIMER_RIGHT_6_SELF_ANY=4.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_POSITION_PENALTY=120.000000 PRIMER_RIGHT_6_POSITION_PENALTY=5.500000 PRIMER_LEFT_6_END_STABILITY=6.1000 PRIMER_RIGHT_6_END_STABILITY=8.2000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=927 PRIMER_PAIR_7_PENALTY=130.117964 PRIMER_LEFT_7_PENALTY=124.973289 PRIMER_RIGHT_7_PENALTY=5.144675 PRIMER_LEFT_7_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_7_SEQUENCE=TACAGTGCTCACCGATCCAG PRIMER_LEFT_7=103,20 PRIMER_RIGHT_7=1028,20 PRIMER_LEFT_7_TM=64.973 PRIMER_RIGHT_7_TM=59.855 PRIMER_LEFT_7_GC_PERCENT=65.000 PRIMER_RIGHT_7_GC_PERCENT=55.000 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_POSITION_PENALTY=120.000000 PRIMER_RIGHT_7_POSITION_PENALTY=5.000000 PRIMER_LEFT_7_END_STABILITY=7.9000 PRIMER_RIGHT_7_END_STABILITY=8.2000 PRIMER_PAIR_7_COMPL_ANY=5.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PAIR_7_PRODUCT_SIZE=926 PRIMER_PAIR_8_PENALTY=130.602129 PRIMER_LEFT_8_PENALTY=122.322207 PRIMER_RIGHT_8_PENALTY=8.279921 PRIMER_LEFT_8_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_8_SEQUENCE=AGTGCTCACCGATCCAGGC PRIMER_LEFT_8=103,19 PRIMER_RIGHT_8=1025,19 PRIMER_LEFT_8_TM=61.322 PRIMER_RIGHT_8_TM=63.780 PRIMER_LEFT_8_GC_PERCENT=63.158 PRIMER_RIGHT_8_GC_PERCENT=63.158 PRIMER_LEFT_8_SELF_ANY=3.00 PRIMER_RIGHT_8_SELF_ANY=4.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_POSITION_PENALTY=120.000000 PRIMER_RIGHT_8_POSITION_PENALTY=3.500000 PRIMER_LEFT_8_END_STABILITY=6.1000 PRIMER_RIGHT_8_END_STABILITY=9.7000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PAIR_8_PRODUCT_SIZE=923 PRIMER_PAIR_9_PENALTY=130.617964 PRIMER_LEFT_9_PENALTY=124.973289 PRIMER_RIGHT_9_PENALTY=5.644675 PRIMER_LEFT_9_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_9_SEQUENCE=CTACAGTGCTCACCGATCCA PRIMER_LEFT_9=103,20 PRIMER_RIGHT_9=1029,20 PRIMER_LEFT_9_TM=64.973 PRIMER_RIGHT_9_TM=59.855 PRIMER_LEFT_9_GC_PERCENT=65.000 PRIMER_RIGHT_9_GC_PERCENT=55.000 PRIMER_LEFT_9_SELF_ANY=3.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=0.00 PRIMER_LEFT_9_POSITION_PENALTY=120.000000 PRIMER_RIGHT_9_POSITION_PENALTY=5.500000 PRIMER_LEFT_9_END_STABILITY=7.9000 PRIMER_RIGHT_9_END_STABILITY=8.1000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=927 PRIMER_PAIR_10_PENALTY=130.636717 PRIMER_LEFT_10_PENALTY=124.973289 PRIMER_RIGHT_10_PENALTY=5.663428 PRIMER_LEFT_10_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_10_SEQUENCE=ACAGTGCTCACCGATCCAG PRIMER_LEFT_10=103,20 PRIMER_RIGHT_10=1027,19 PRIMER_LEFT_10_TM=64.973 PRIMER_RIGHT_10_TM=59.837 PRIMER_LEFT_10_GC_PERCENT=65.000 PRIMER_RIGHT_10_GC_PERCENT=57.895 PRIMER_LEFT_10_SELF_ANY=3.00 PRIMER_RIGHT_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=120.000000 PRIMER_RIGHT_10_POSITION_PENALTY=4.500000 PRIMER_LEFT_10_END_STABILITY=7.9000 PRIMER_RIGHT_10_END_STABILITY=8.2000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PAIR_10_PRODUCT_SIZE=925 PRIMER_PAIR_11_PENALTY=130.735297 PRIMER_LEFT_11_PENALTY=122.322207 PRIMER_RIGHT_11_PENALTY=8.413090 PRIMER_LEFT_11_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_11_SEQUENCE=AGGCTACAGTGCTCACCGA PRIMER_LEFT_11=103,19 PRIMER_RIGHT_11=1032,19 PRIMER_LEFT_11_TM=61.322 PRIMER_RIGHT_11_TM=59.587 PRIMER_LEFT_11_GC_PERCENT=63.158 PRIMER_RIGHT_11_GC_PERCENT=57.895 PRIMER_LEFT_11_SELF_ANY=3.00 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=120.000000 PRIMER_RIGHT_11_POSITION_PENALTY=7.000000 PRIMER_LEFT_11_END_STABILITY=6.1000 PRIMER_RIGHT_11_END_STABILITY=9.6000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=3.00 PRIMER_PAIR_11_PRODUCT_SIZE=930 PRIMER_PAIR_12_PENALTY=130.977381 PRIMER_LEFT_12_PENALTY=122.322207 PRIMER_RIGHT_12_PENALTY=8.655174 PRIMER_LEFT_12_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_12_SEQUENCE=GGCTACAGTGCTCACCGAT PRIMER_LEFT_12=103,19 PRIMER_RIGHT_12=1031,19 PRIMER_LEFT_12_TM=61.322 PRIMER_RIGHT_12_TM=58.845 PRIMER_LEFT_12_GC_PERCENT=63.158 PRIMER_RIGHT_12_GC_PERCENT=57.895 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=120.000000 PRIMER_RIGHT_12_POSITION_PENALTY=6.500000 PRIMER_LEFT_12_END_STABILITY=6.1000 PRIMER_RIGHT_12_END_STABILITY=9.8000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PAIR_12_PRODUCT_SIZE=929 PRIMER_PAIR_13_PENALTY=131.729338 PRIMER_LEFT_13_PENALTY=122.322207 PRIMER_RIGHT_13_PENALTY=9.407131 PRIMER_LEFT_13_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_13_SEQUENCE=GCAGGCTACAGTGCTCACC PRIMER_LEFT_13=103,19 PRIMER_RIGHT_13=1034,19 PRIMER_LEFT_13_TM=61.322 PRIMER_RIGHT_13_TM=59.593 PRIMER_LEFT_13_GC_PERCENT=63.158 PRIMER_RIGHT_13_GC_PERCENT=63.158 PRIMER_LEFT_13_SELF_ANY=3.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=120.000000 PRIMER_RIGHT_13_POSITION_PENALTY=8.000000 PRIMER_LEFT_13_END_STABILITY=6.1000 PRIMER_RIGHT_13_END_STABILITY=7.9000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=3.00 PRIMER_PAIR_13_PRODUCT_SIZE=932 PRIMER_PAIR_14_PENALTY=131.806548 PRIMER_LEFT_14_PENALTY=124.973289 PRIMER_RIGHT_14_PENALTY=6.833259 PRIMER_LEFT_14_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_14_SEQUENCE=GCTACAGTGCTCACCGATCC PRIMER_LEFT_14=103,20 PRIMER_RIGHT_14=1030,20 PRIMER_LEFT_14_TM=64.973 PRIMER_RIGHT_14_TM=60.833 PRIMER_LEFT_14_GC_PERCENT=65.000 PRIMER_RIGHT_14_GC_PERCENT=60.000 PRIMER_LEFT_14_SELF_ANY=3.00 PRIMER_RIGHT_14_SELF_ANY=4.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=120.000000 PRIMER_RIGHT_14_POSITION_PENALTY=6.000000 PRIMER_LEFT_14_END_STABILITY=7.9000 PRIMER_RIGHT_14_END_STABILITY=7.8000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=1.00 PRIMER_PAIR_14_PRODUCT_SIZE=928 PRIMER_PAIR_15_PENALTY=132.231362 PRIMER_LEFT_15_PENALTY=124.973289 PRIMER_RIGHT_15_PENALTY=7.258073 PRIMER_LEFT_15_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_15_SEQUENCE=TACAGTGCTCACCGATCCA PRIMER_LEFT_15=103,20 PRIMER_RIGHT_15=1028,19 PRIMER_LEFT_15_TM=64.973 PRIMER_RIGHT_15_TM=58.742 PRIMER_LEFT_15_GC_PERCENT=65.000 PRIMER_RIGHT_15_GC_PERCENT=52.632 PRIMER_LEFT_15_SELF_ANY=3.00 PRIMER_RIGHT_15_SELF_ANY=4.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_POSITION_PENALTY=120.000000 PRIMER_RIGHT_15_POSITION_PENALTY=5.000000 PRIMER_LEFT_15_END_STABILITY=7.9000 PRIMER_RIGHT_15_END_STABILITY=8.1000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PAIR_15_PRODUCT_SIZE=926 PRIMER_PAIR_16_PENALTY=132.332426 PRIMER_LEFT_16_PENALTY=124.973289 PRIMER_RIGHT_16_PENALTY=7.359137 PRIMER_LEFT_16_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_16_SEQUENCE=CTACAGTGCTCACCGATCCAG PRIMER_LEFT_16=103,20 PRIMER_RIGHT_16=1029,21 PRIMER_LEFT_16_TM=64.973 PRIMER_RIGHT_16_TM=60.859 PRIMER_LEFT_16_GC_PERCENT=65.000 PRIMER_RIGHT_16_GC_PERCENT=57.143 PRIMER_LEFT_16_SELF_ANY=3.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=1.00 PRIMER_LEFT_16_POSITION_PENALTY=120.000000 PRIMER_RIGHT_16_POSITION_PENALTY=5.500000 PRIMER_LEFT_16_END_STABILITY=7.9000 PRIMER_RIGHT_16_END_STABILITY=8.2000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PAIR_16_PRODUCT_SIZE=927 PRIMER_PAIR_17_PENALTY=132.392649 PRIMER_LEFT_17_PENALTY=122.322207 PRIMER_RIGHT_17_PENALTY=10.070442 PRIMER_LEFT_17_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_17_SEQUENCE=CAGGCTACAGTGCTCACCGA PRIMER_LEFT_17=103,19 PRIMER_RIGHT_17=1033,20 PRIMER_LEFT_17_TM=61.322 PRIMER_RIGHT_17_TM=62.570 PRIMER_LEFT_17_GC_PERCENT=63.158 PRIMER_RIGHT_17_GC_PERCENT=60.000 PRIMER_LEFT_17_SELF_ANY=3.00 PRIMER_RIGHT_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=120.000000 PRIMER_RIGHT_17_POSITION_PENALTY=7.500000 PRIMER_LEFT_17_END_STABILITY=6.1000 PRIMER_RIGHT_17_END_STABILITY=9.6000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=3.00 PRIMER_PAIR_17_PRODUCT_SIZE=931 PRIMER_PAIR_18_PENALTY=132.859566 PRIMER_LEFT_18_PENALTY=124.973289 PRIMER_RIGHT_18_PENALTY=7.886276 PRIMER_LEFT_18_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_18_SEQUENCE=CAGTGCTCACCGATCCAGG PRIMER_LEFT_18=103,20 PRIMER_RIGHT_18=1026,19 PRIMER_LEFT_18_TM=64.973 PRIMER_RIGHT_18_TM=62.886 PRIMER_LEFT_18_GC_PERCENT=65.000 PRIMER_RIGHT_18_GC_PERCENT=63.158 PRIMER_LEFT_18_SELF_ANY=3.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=3.00 PRIMER_LEFT_18_POSITION_PENALTY=120.000000 PRIMER_RIGHT_18_POSITION_PENALTY=4.000000 PRIMER_LEFT_18_END_STABILITY=7.9000 PRIMER_RIGHT_18_END_STABILITY=9.7000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=3.00 PRIMER_PAIR_18_PRODUCT_SIZE=924 PRIMER_PAIR_19_PENALTY=132.985916 PRIMER_LEFT_19_PENALTY=122.322207 PRIMER_RIGHT_19_PENALTY=10.663709 PRIMER_LEFT_19_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_19_SEQUENCE=GCTACAGTGCTCACCGATCCA PRIMER_LEFT_19=103,19 PRIMER_RIGHT_19=1030,21 PRIMER_LEFT_19_TM=61.322 PRIMER_RIGHT_19_TM=63.664 PRIMER_LEFT_19_GC_PERCENT=63.158 PRIMER_RIGHT_19_GC_PERCENT=57.143 PRIMER_LEFT_19_SELF_ANY=3.00 PRIMER_RIGHT_19_SELF_ANY=4.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_POSITION_PENALTY=120.000000 PRIMER_RIGHT_19_POSITION_PENALTY=6.000000 PRIMER_LEFT_19_END_STABILITY=6.1000 PRIMER_RIGHT_19_END_STABILITY=8.1000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PAIR_19_PRODUCT_SIZE=928 = SEQUENCE_ID=L23959/DP1 P3_COMMENT=Homo sapiens E2F-related transcription factor (DP-1) mRNA, complete cds. CDS_38..1270 SEQUENCE_START_CODON_POSITION=37 SEQUENCE_TEMPLATE=GGAATTCCGTAGCTATTGATTTCCCGGATCTGGTAACATGGCAAAAGATGCCGGTCTAATTGAAGCCAACGGAGAACTCAAGGTCTTCATAGACCAGAACCTTAGTCCCGGGAAAGGCGTGGTGTCCCTCGTGGCCGTTCACCCCTCCACCGTCAACCCGCTCGGGAAGCAGCTCTTGCCAAAAACCTTTGGACAGTCCAATGTCAACATTGCCCAGCAAGTGGTAATTGGTACGCCTCAGAGACCGGCAGCGTCAAACACCCTGGTGGTAGGAAGCCCACACACCCCCAGCACTCACTTTGCCTCTCAGAACCAGCCTTCCGACTCCTCACCTTGGTCTGCCGGGAAGCGCAACAGGAAAGGAGAGAAGAATGGCAAGGGCCTACGGCATTTCTCCATGAAGGTCTGCGAGAAGGTGCAGAGGAAAGGGACCACTTCCTACAACGAAGTGGCAGACGAGCTGGTTGCGGAGTTCAGTGCTGCCGACAACCACATCTTACCAAACGAGTCAGCTTATGACCAGAAAAACATAAGACGGCGCGTCTACGATGCCTTAAACGTGCTAATGGCCATGAACATCATCTCCAAGGAGAAGAAGGAGATCAAGTGGATTGGTCTGCCCACCAACTCGGCTCAGGAATGTCAGAACTTAGAGGTGGAAAGACAGAGGAGACTTGAAAGAATAAAACAGAAACAGTCTCAACTTCAAGAACTTATTCTACAGCAAATTGCCTTCAAGAACCTGGTGCAGAGAAACCGGCATGCGGAGCAGCAGGCCAGCCGGCCACCGCCACCCAACTCAGTCATCCACCTGCCCTTCATCATCGTCAACACCAGCAAGAAGACGGTCATCGACTGCAGCATCTCCAATGACAAATTTGAGTATCTGTTTAATTTTGACAACACATTTGAAATCCACGATGACATAGAAGTGCTGAAGCGGATGGGCATGGCTTGCGGGCTGGAGTCGGGGAGCTGCTCTGCCGAAGACCTTAAAATGGCCAGAAGTCTGGTCCCCAAGGCTCTGGAGCCATACGTGACAGAAATGGCTCAGGGAACTGTTGGAGGCGTGTTCATCACGACGGCAGGTTCCACGTCTAACGGCACAAGGTTCTCTGCCAGTGACCTGACCAACGGTGCAGATGGGATGCTGGCCACAAGCTCCAATGGGTCTCAGTACAGCGGCTCCAGGGTGGAGACTCCGGTGTCCTACGTCGGGGAGGACGACGAGGAGGACGATGACTTCAACGAGAATGACGAGGACGACTGACGTCCTCCCCACTTCAGATTCGGCTTCAGGAAAACGTTTAGCGAAAAGAAACTTTTTTTTTAATGTGGGTTTTCTGTTTCCTTTTGGCCTAGTCCCAAGAAGATATTGGTAAGCTATTGAATTTAGATATGCACCTCTGATAAGCAAGGATTGTTTCCCGTAGATTAGGA PRIMER_LEFT_EXPLAIN=considered 11963, would not amplify any of the ORF 8309, GC content failed 29, low tm 476, high tm 2059, high 3' stability 47, ok 1043 PRIMER_RIGHT_EXPLAIN=considered 11769, GC content failed 99, low tm 1773, high tm 6322, long poly-x seq 25, high 3' stability 49, ok 3501 PRIMER_PAIR_EXPLAIN=considered 23, ok 23 PRIMER_STOP_CODON_POSITION=1267 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=4.447526 PRIMER_LEFT_0_PENALTY=4.039125 PRIMER_RIGHT_0_PENALTY=0.408401 PRIMER_LEFT_0_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_0_SEQUENCE=AGTCGTCCTCGTCATTCTCG PRIMER_LEFT_0=37,20 PRIMER_RIGHT_0=1267,20 PRIMER_LEFT_0_TM=64.039 PRIMER_RIGHT_0_TM=60.408 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=8.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=1231 PRIMER_PAIR_1_PENALTY=4.666064 PRIMER_LEFT_1_PENALTY=4.257663 PRIMER_RIGHT_1_PENALTY=0.408401 PRIMER_LEFT_1_SEQUENCE=ATGGCAAAAGATGCCGGTC PRIMER_RIGHT_1_SEQUENCE=AGTCGTCCTCGTCATTCTCG PRIMER_LEFT_1=37,19 PRIMER_RIGHT_1=1267,20 PRIMER_LEFT_1_TM=63.258 PRIMER_RIGHT_1_TM=60.408 PRIMER_LEFT_1_GC_PERCENT=52.632 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=1231 PRIMER_PAIR_2_PENALTY=5.312234 PRIMER_LEFT_2_PENALTY=4.903833 PRIMER_RIGHT_2_PENALTY=0.408401 PRIMER_LEFT_2_SEQUENCE=ATGGCAAAAGATGCCGGTCTA PRIMER_RIGHT_2_SEQUENCE=AGTCGTCCTCGTCATTCTCG PRIMER_LEFT_2=37,21 PRIMER_RIGHT_2=1267,20 PRIMER_LEFT_2_TM=63.904 PRIMER_RIGHT_2_TM=60.408 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=0.000000 PRIMER_LEFT_2_END_STABILITY=5.4000 PRIMER_RIGHT_2_END_STABILITY=8.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=1231 PRIMER_PAIR_3_PENALTY=6.160526 PRIMER_LEFT_3_PENALTY=4.039125 PRIMER_RIGHT_3_PENALTY=2.121401 PRIMER_LEFT_3_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_3_SEQUENCE=CAGTCGTCCTCGTCATTCTC PRIMER_LEFT_3=37,20 PRIMER_RIGHT_3=1268,20 PRIMER_LEFT_3_TM=64.039 PRIMER_RIGHT_3_TM=58.379 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.500000 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=1232 PRIMER_PAIR_4_PENALTY=6.274302 PRIMER_LEFT_4_PENALTY=4.039125 PRIMER_RIGHT_4_PENALTY=2.235177 PRIMER_LEFT_4_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_4_SEQUENCE=AGTCGTCCTCGTCATTCTCGT PRIMER_LEFT_4=37,20 PRIMER_RIGHT_4=1267,21 PRIMER_LEFT_4_TM=64.039 PRIMER_RIGHT_4_TM=61.235 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=0.000000 PRIMER_LEFT_4_END_STABILITY=7.6000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=1231 PRIMER_PAIR_5_PENALTY=6.379064 PRIMER_LEFT_5_PENALTY=4.257663 PRIMER_RIGHT_5_PENALTY=2.121401 PRIMER_LEFT_5_SEQUENCE=ATGGCAAAAGATGCCGGTC PRIMER_RIGHT_5_SEQUENCE=CAGTCGTCCTCGTCATTCTC PRIMER_LEFT_5=37,19 PRIMER_RIGHT_5=1268,20 PRIMER_LEFT_5_TM=63.258 PRIMER_RIGHT_5_TM=58.379 PRIMER_LEFT_5_GC_PERCENT=52.632 PRIMER_RIGHT_5_GC_PERCENT=55.000 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=2.00 PRIMER_LEFT_5_SELF_END=2.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=0.500000 PRIMER_LEFT_5_END_STABILITY=9.6000 PRIMER_RIGHT_5_END_STABILITY=6.7000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=1232 PRIMER_PAIR_6_PENALTY=6.440768 PRIMER_LEFT_6_PENALTY=4.039125 PRIMER_RIGHT_6_PENALTY=2.401642 PRIMER_LEFT_6_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_6_SEQUENCE=TCAGTCGTCCTCGTCATTCTC PRIMER_LEFT_6=37,20 PRIMER_RIGHT_6=1269,21 PRIMER_LEFT_6_TM=64.039 PRIMER_RIGHT_6_TM=60.402 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=52.381 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=2.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=1.000000 PRIMER_LEFT_6_END_STABILITY=7.6000 PRIMER_RIGHT_6_END_STABILITY=6.7000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PAIR_6_PRODUCT_SIZE=1233 PRIMER_PAIR_7_PENALTY=6.492840 PRIMER_LEFT_7_PENALTY=4.257663 PRIMER_RIGHT_7_PENALTY=2.235177 PRIMER_LEFT_7_SEQUENCE=ATGGCAAAAGATGCCGGTC PRIMER_RIGHT_7_SEQUENCE=AGTCGTCCTCGTCATTCTCGT PRIMER_LEFT_7=37,19 PRIMER_RIGHT_7=1267,21 PRIMER_LEFT_7_TM=63.258 PRIMER_RIGHT_7_TM=61.235 PRIMER_LEFT_7_GC_PERCENT=52.632 PRIMER_RIGHT_7_GC_PERCENT=52.381 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=2.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=0.000000 PRIMER_LEFT_7_END_STABILITY=9.6000 PRIMER_RIGHT_7_END_STABILITY=8.1000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PAIR_7_PRODUCT_SIZE=1231 PRIMER_PAIR_8_PENALTY=6.659306 PRIMER_LEFT_8_PENALTY=4.257663 PRIMER_RIGHT_8_PENALTY=2.401642 PRIMER_LEFT_8_SEQUENCE=ATGGCAAAAGATGCCGGTC PRIMER_RIGHT_8_SEQUENCE=TCAGTCGTCCTCGTCATTCTC PRIMER_LEFT_8=37,19 PRIMER_RIGHT_8=1269,21 PRIMER_LEFT_8_TM=63.258 PRIMER_RIGHT_8_TM=60.402 PRIMER_LEFT_8_GC_PERCENT=52.632 PRIMER_RIGHT_8_GC_PERCENT=52.381 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=2.00 PRIMER_LEFT_8_SELF_END=2.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=1.000000 PRIMER_LEFT_8_END_STABILITY=9.6000 PRIMER_RIGHT_8_END_STABILITY=6.7000 PRIMER_PAIR_8_COMPL_ANY=4.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PAIR_8_PRODUCT_SIZE=1233 PRIMER_PAIR_9_PENALTY=6.660526 PRIMER_LEFT_9_PENALTY=4.039125 PRIMER_RIGHT_9_PENALTY=2.621401 PRIMER_LEFT_9_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_9_SEQUENCE=TCAGTCGTCCTCGTCATTCT PRIMER_LEFT_9=37,20 PRIMER_RIGHT_9=1269,20 PRIMER_LEFT_9_TM=64.039 PRIMER_RIGHT_9_TM=58.379 PRIMER_LEFT_9_GC_PERCENT=50.000 PRIMER_RIGHT_9_GC_PERCENT=50.000 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=2.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=0.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=1.000000 PRIMER_LEFT_9_END_STABILITY=7.6000 PRIMER_RIGHT_9_END_STABILITY=6.6000 PRIMER_PAIR_9_COMPL_ANY=4.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PAIR_9_PRODUCT_SIZE=1233 PRIMER_PAIR_10_PENALTY=6.879064 PRIMER_LEFT_10_PENALTY=4.257663 PRIMER_RIGHT_10_PENALTY=2.621401 PRIMER_LEFT_10_SEQUENCE=ATGGCAAAAGATGCCGGTC PRIMER_RIGHT_10_SEQUENCE=TCAGTCGTCCTCGTCATTCT PRIMER_LEFT_10=37,19 PRIMER_RIGHT_10=1269,20 PRIMER_LEFT_10_TM=63.258 PRIMER_RIGHT_10_TM=58.379 PRIMER_LEFT_10_GC_PERCENT=52.632 PRIMER_RIGHT_10_GC_PERCENT=50.000 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=2.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=1.000000 PRIMER_LEFT_10_END_STABILITY=9.6000 PRIMER_RIGHT_10_END_STABILITY=6.6000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=1233 PRIMER_PAIR_11_PENALTY=7.025234 PRIMER_LEFT_11_PENALTY=4.903833 PRIMER_RIGHT_11_PENALTY=2.121401 PRIMER_LEFT_11_SEQUENCE=ATGGCAAAAGATGCCGGTCTA PRIMER_RIGHT_11_SEQUENCE=CAGTCGTCCTCGTCATTCTC PRIMER_LEFT_11=37,21 PRIMER_RIGHT_11=1268,20 PRIMER_LEFT_11_TM=63.904 PRIMER_RIGHT_11_TM=58.379 PRIMER_LEFT_11_GC_PERCENT=47.619 PRIMER_RIGHT_11_GC_PERCENT=55.000 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=2.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=0.500000 PRIMER_LEFT_11_END_STABILITY=5.4000 PRIMER_RIGHT_11_END_STABILITY=6.7000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=1.00 PRIMER_PAIR_11_PRODUCT_SIZE=1232 PRIMER_PAIR_12_PENALTY=7.139009 PRIMER_LEFT_12_PENALTY=4.903833 PRIMER_RIGHT_12_PENALTY=2.235177 PRIMER_LEFT_12_SEQUENCE=ATGGCAAAAGATGCCGGTCTA PRIMER_RIGHT_12_SEQUENCE=AGTCGTCCTCGTCATTCTCGT PRIMER_LEFT_12=37,21 PRIMER_RIGHT_12=1267,21 PRIMER_LEFT_12_TM=63.904 PRIMER_RIGHT_12_TM=61.235 PRIMER_LEFT_12_GC_PERCENT=47.619 PRIMER_RIGHT_12_GC_PERCENT=52.381 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=2.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=0.000000 PRIMER_LEFT_12_END_STABILITY=5.4000 PRIMER_RIGHT_12_END_STABILITY=8.1000 PRIMER_PAIR_12_COMPL_ANY=4.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PAIR_12_PRODUCT_SIZE=1231 PRIMER_PAIR_13_PENALTY=7.202553 PRIMER_LEFT_13_PENALTY=4.039125 PRIMER_RIGHT_13_PENALTY=3.163428 PRIMER_LEFT_13_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_13_SEQUENCE=CGTCAGTCGTCCTCGTCAT PRIMER_LEFT_13=37,20 PRIMER_RIGHT_13=1271,19 PRIMER_LEFT_13_TM=64.039 PRIMER_RIGHT_13_TM=59.837 PRIMER_LEFT_13_GC_PERCENT=50.000 PRIMER_RIGHT_13_GC_PERCENT=57.895 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=3.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=2.000000 PRIMER_LEFT_13_END_STABILITY=7.6000 PRIMER_RIGHT_13_END_STABILITY=6.3000 PRIMER_PAIR_13_COMPL_ANY=4.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PAIR_13_PRODUCT_SIZE=1235 PRIMER_PAIR_14_PENALTY=7.219577 PRIMER_LEFT_14_PENALTY=4.039125 PRIMER_RIGHT_14_PENALTY=3.180452 PRIMER_LEFT_14_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_14_SEQUENCE=GTCAGTCGTCCTCGTCATTCT PRIMER_LEFT_14=37,20 PRIMER_RIGHT_14=1270,21 PRIMER_LEFT_14_TM=64.039 PRIMER_RIGHT_14_TM=59.320 PRIMER_LEFT_14_GC_PERCENT=50.000 PRIMER_RIGHT_14_GC_PERCENT=52.381 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=2.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=1.500000 PRIMER_LEFT_14_END_STABILITY=7.6000 PRIMER_RIGHT_14_END_STABILITY=6.6000 PRIMER_PAIR_14_COMPL_ANY=4.00 PRIMER_PAIR_14_COMPL_END=0.00 PRIMER_PAIR_14_PRODUCT_SIZE=1234 PRIMER_PAIR_15_PENALTY=7.284820 PRIMER_LEFT_15_PENALTY=4.039125 PRIMER_RIGHT_15_PENALTY=3.245695 PRIMER_LEFT_15_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_15_SEQUENCE=ACGTCAGTCGTCCTCGTCAT PRIMER_LEFT_15=37,20 PRIMER_RIGHT_15=1272,20 PRIMER_LEFT_15_TM=64.039 PRIMER_RIGHT_15_TM=60.746 PRIMER_LEFT_15_GC_PERCENT=50.000 PRIMER_RIGHT_15_GC_PERCENT=55.000 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=5.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=2.500000 PRIMER_LEFT_15_END_STABILITY=7.6000 PRIMER_RIGHT_15_END_STABILITY=6.3000 PRIMER_PAIR_15_COMPL_ANY=4.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PAIR_15_PRODUCT_SIZE=1236 PRIMER_PAIR_16_PENALTY=7.305475 PRIMER_LEFT_16_PENALTY=4.903833 PRIMER_RIGHT_16_PENALTY=2.401642 PRIMER_LEFT_16_SEQUENCE=ATGGCAAAAGATGCCGGTCTA PRIMER_RIGHT_16_SEQUENCE=TCAGTCGTCCTCGTCATTCTC PRIMER_LEFT_16=37,21 PRIMER_RIGHT_16=1269,21 PRIMER_LEFT_16_TM=63.904 PRIMER_RIGHT_16_TM=60.402 PRIMER_LEFT_16_GC_PERCENT=47.619 PRIMER_RIGHT_16_GC_PERCENT=52.381 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=2.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=1.000000 PRIMER_LEFT_16_END_STABILITY=5.4000 PRIMER_RIGHT_16_END_STABILITY=6.7000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PAIR_16_PRODUCT_SIZE=1233 PRIMER_PAIR_17_PENALTY=7.310448 PRIMER_LEFT_17_PENALTY=6.902047 PRIMER_RIGHT_17_PENALTY=0.408401 PRIMER_LEFT_17_SEQUENCE=ATGGCAAAAGATGCCGGTCTAA PRIMER_RIGHT_17_SEQUENCE=AGTCGTCCTCGTCATTCTCG PRIMER_LEFT_17=37,22 PRIMER_RIGHT_17=1267,20 PRIMER_LEFT_17_TM=64.902 PRIMER_RIGHT_17_TM=60.408 PRIMER_LEFT_17_GC_PERCENT=45.455 PRIMER_RIGHT_17_GC_PERCENT=55.000 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=2.00 PRIMER_LEFT_17_SELF_END=1.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=0.000000 PRIMER_LEFT_17_END_STABILITY=6.0000 PRIMER_RIGHT_17_END_STABILITY=8.4000 PRIMER_PAIR_17_COMPL_ANY=4.00 PRIMER_PAIR_17_COMPL_END=0.00 PRIMER_PAIR_17_PRODUCT_SIZE=1231 PRIMER_PAIR_18_PENALTY=7.318367 PRIMER_LEFT_18_PENALTY=4.039125 PRIMER_RIGHT_18_PENALTY=3.279241 PRIMER_LEFT_18_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_18_SEQUENCE=CGTCAGTCGTCCTCGTCATT PRIMER_LEFT_18=37,20 PRIMER_RIGHT_18=1271,20 PRIMER_LEFT_18_TM=64.039 PRIMER_RIGHT_18_TM=61.279 PRIMER_LEFT_18_GC_PERCENT=50.000 PRIMER_RIGHT_18_GC_PERCENT=55.000 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=3.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=1.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=2.000000 PRIMER_LEFT_18_END_STABILITY=7.6000 PRIMER_RIGHT_18_END_STABILITY=6.9000 PRIMER_PAIR_18_COMPL_ANY=4.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PAIR_18_PRODUCT_SIZE=1235 PRIMER_PAIR_19_PENALTY=7.322844 PRIMER_LEFT_19_PENALTY=4.039125 PRIMER_RIGHT_19_PENALTY=3.283719 PRIMER_LEFT_19_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_19_SEQUENCE=GTCAGTCGTCCTCGTCATTC PRIMER_LEFT_19=37,20 PRIMER_RIGHT_19=1270,20 PRIMER_LEFT_19_TM=64.039 PRIMER_RIGHT_19_TM=58.216 PRIMER_LEFT_19_GC_PERCENT=50.000 PRIMER_RIGHT_19_GC_PERCENT=55.000 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=2.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=1.500000 PRIMER_LEFT_19_END_STABILITY=7.6000 PRIMER_RIGHT_19_END_STABILITY=6.9000 PRIMER_PAIR_19_COMPL_ANY=4.00 PRIMER_PAIR_19_COMPL_END=0.00 PRIMER_PAIR_19_PRODUCT_SIZE=1234 = SEQUENCE_ID=X98032/E6AP P3_COMMENT=H.sapiens mRNA for E6-AP isoform-I. CDS_1..2559 SEQUENCE_START_CODON_POSITION=0 SEQUENCE_TEMPLATE=ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAGAACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAGAAAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAAATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTGGTACAGAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTCAAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGGTGATAGCTCACAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATGTGTCTGTGGATATTGATGCCATTAGAAGGGTCTACACCAGATTGCTCTCTAATGAAAAAATTGAAACTGCCTTTCTCAATGCACTTGTATATTTGTCACCTAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGATCCTAATTATCTGAATTTGTTCATTATCGGAATGGAGAATAGAAATCTCCACAGTCCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAAGCTACCCCTTGCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACAATGCAGACCAGATTCGGAGAATGATGGAGACATTTCAGCAACTTATTACTTATAAAGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTTTACTATGCAAATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAAGAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGAAGAAAGAAGAAACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAACTTGGTGTTAAAACCCTGGATTGTCGAAAACCACTTATCCCTTTTGAAGAGTTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGATTATACTTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGAATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCAAGGACAGCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATATCATAGATGATGCACTTGTCCGGCTAGAGATGATCGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAATCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTGTTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGATTGGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTTCGTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAAGATTTATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAAAATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTTTATTCTGACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGGCTTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAAAGTACTTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCAGGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACAGATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTGTGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTGCTTTAATGTGCTTTTACTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATCACGTATGCCAAAGGATTTGGCATGCTGTAA PRIMER_LEFT_EXPLAIN=considered 21507, would not amplify any of the ORF 14282, GC content failed 55, low tm 3071, high tm 1071, high end compl 6, long poly-x seq 102, high 3' stability 49, ok 2871 PRIMER_RIGHT_EXPLAIN=considered 21345, GC content failed 229, low tm 9108, high tm 3197, high end compl 7, long poly-x seq 106, high 3' stability 53, ok 8645 PRIMER_PAIR_EXPLAIN=considered 27, high end compl 6, ok 21 PRIMER_STOP_CODON_POSITION=2556 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=6.753748 PRIMER_LEFT_0_PENALTY=4.494664 PRIMER_RIGHT_0_PENALTY=2.259083 PRIMER_LEFT_0_SEQUENCE=ATGAAGCGAGCAGCTGCAAA PRIMER_RIGHT_0_SEQUENCE=TACAGCATGCCAAATCCTTT PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=2557,20 PRIMER_LEFT_0_TM=64.495 PRIMER_RIGHT_0_TM=58.241 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.500000 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=2558 PRIMER_PAIR_1_PENALTY=6.894578 PRIMER_LEFT_1_PENALTY=4.494664 PRIMER_RIGHT_1_PENALTY=2.399914 PRIMER_LEFT_1_SEQUENCE=ATGAAGCGAGCAGCTGCAAA PRIMER_RIGHT_1_SEQUENCE=TTACAGCATGCCAAATCCTTT PRIMER_LEFT_1=0,20 PRIMER_RIGHT_1=2558,21 PRIMER_LEFT_1_TM=64.495 PRIMER_RIGHT_1_TM=59.600 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=1.000000 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=2559 PRIMER_PAIR_2_PENALTY=7.253748 PRIMER_LEFT_2_PENALTY=4.494664 PRIMER_RIGHT_2_PENALTY=2.759083 PRIMER_LEFT_2_SEQUENCE=ATGAAGCGAGCAGCTGCAAA PRIMER_RIGHT_2_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=2558,20 PRIMER_LEFT_2_TM=64.495 PRIMER_RIGHT_2_TM=58.241 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=1.000000 PRIMER_LEFT_2_END_STABILITY=8.8000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=2559 PRIMER_PAIR_3_PENALTY=7.335632 PRIMER_LEFT_3_PENALTY=4.494664 PRIMER_RIGHT_3_PENALTY=2.840968 PRIMER_LEFT_3_SEQUENCE=ATGAAGCGAGCAGCTGCAAA PRIMER_RIGHT_3_SEQUENCE=ACAGCATGCCAAATCCTTT PRIMER_LEFT_3=0,20 PRIMER_RIGHT_3=2556,19 PRIMER_LEFT_3_TM=64.495 PRIMER_RIGHT_3_TM=58.159 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=42.105 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.000000 PRIMER_LEFT_3_END_STABILITY=8.8000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=2557 PRIMER_PAIR_4_PENALTY=127.102182 PRIMER_LEFT_4_PENALTY=124.343098 PRIMER_RIGHT_4_PENALTY=2.759083 PRIMER_LEFT_4_SEQUENCE=CGAGCAGCTGCAAAGCATC PRIMER_RIGHT_4_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_4=6,19 PRIMER_RIGHT_4=2558,20 PRIMER_LEFT_4_TM=63.343 PRIMER_RIGHT_4_TM=58.241 PRIMER_LEFT_4_GC_PERCENT=57.895 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_POSITION_PENALTY=120.000000 PRIMER_RIGHT_4_POSITION_PENALTY=1.000000 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=2553 PRIMER_PAIR_5_PENALTY=127.764493 PRIMER_LEFT_5_PENALTY=125.005410 PRIMER_RIGHT_5_PENALTY=2.759083 PRIMER_LEFT_5_SEQUENCE=CGAGCAGCTGCAAAGCATCTA PRIMER_RIGHT_5_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_5=6,21 PRIMER_RIGHT_5=2558,20 PRIMER_LEFT_5_TM=64.005 PRIMER_RIGHT_5_TM=58.241 PRIMER_LEFT_5_GC_PERCENT=52.381 PRIMER_RIGHT_5_GC_PERCENT=40.000 PRIMER_LEFT_5_SELF_ANY=8.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_POSITION_PENALTY=120.000000 PRIMER_RIGHT_5_POSITION_PENALTY=1.000000 PRIMER_LEFT_5_END_STABILITY=5.6000 PRIMER_RIGHT_5_END_STABILITY=8.2000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PAIR_5_PRODUCT_SIZE=2553 PRIMER_PAIR_6_PENALTY=131.139105 PRIMER_LEFT_6_PENALTY=128.880021 PRIMER_RIGHT_6_PENALTY=2.259083 PRIMER_LEFT_6_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_6_SEQUENCE=TACAGCATGCCAAATCCTTT PRIMER_LEFT_6=6,24 PRIMER_RIGHT_6=2557,20 PRIMER_LEFT_6_TM=64.880 PRIMER_RIGHT_6_TM=58.241 PRIMER_LEFT_6_GC_PERCENT=45.833 PRIMER_RIGHT_6_GC_PERCENT=40.000 PRIMER_LEFT_6_SELF_ANY=8.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=3.00 PRIMER_LEFT_6_POSITION_PENALTY=120.000000 PRIMER_RIGHT_6_POSITION_PENALTY=0.500000 PRIMER_LEFT_6_END_STABILITY=5.2000 PRIMER_RIGHT_6_END_STABILITY=8.5000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=2552 PRIMER_PAIR_7_PENALTY=131.279935 PRIMER_LEFT_7_PENALTY=128.880021 PRIMER_RIGHT_7_PENALTY=2.399914 PRIMER_LEFT_7_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_7_SEQUENCE=TTACAGCATGCCAAATCCTTT PRIMER_LEFT_7=6,24 PRIMER_RIGHT_7=2558,21 PRIMER_LEFT_7_TM=64.880 PRIMER_RIGHT_7_TM=59.600 PRIMER_LEFT_7_GC_PERCENT=45.833 PRIMER_RIGHT_7_GC_PERCENT=38.095 PRIMER_LEFT_7_SELF_ANY=8.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=3.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_POSITION_PENALTY=120.000000 PRIMER_RIGHT_7_POSITION_PENALTY=1.000000 PRIMER_LEFT_7_END_STABILITY=5.2000 PRIMER_RIGHT_7_END_STABILITY=8.5000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PAIR_7_PRODUCT_SIZE=2553 PRIMER_PAIR_8_PENALTY=131.639105 PRIMER_LEFT_8_PENALTY=128.880021 PRIMER_RIGHT_8_PENALTY=2.759083 PRIMER_LEFT_8_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_8_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_8=6,24 PRIMER_RIGHT_8=2558,20 PRIMER_LEFT_8_TM=64.880 PRIMER_RIGHT_8_TM=58.241 PRIMER_LEFT_8_GC_PERCENT=45.833 PRIMER_RIGHT_8_GC_PERCENT=40.000 PRIMER_LEFT_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=1.00 PRIMER_LEFT_8_POSITION_PENALTY=120.000000 PRIMER_RIGHT_8_POSITION_PENALTY=1.000000 PRIMER_LEFT_8_END_STABILITY=5.2000 PRIMER_RIGHT_8_END_STABILITY=8.2000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PAIR_8_PRODUCT_SIZE=2553 PRIMER_PAIR_9_PENALTY=131.720989 PRIMER_LEFT_9_PENALTY=128.880021 PRIMER_RIGHT_9_PENALTY=2.840968 PRIMER_LEFT_9_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_9_SEQUENCE=ACAGCATGCCAAATCCTTT PRIMER_LEFT_9=6,24 PRIMER_RIGHT_9=2556,19 PRIMER_LEFT_9_TM=64.880 PRIMER_RIGHT_9_TM=58.159 PRIMER_LEFT_9_GC_PERCENT=45.833 PRIMER_RIGHT_9_GC_PERCENT=42.105 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_POSITION_PENALTY=120.000000 PRIMER_RIGHT_9_POSITION_PENALTY=0.000000 PRIMER_LEFT_9_END_STABILITY=5.2000 PRIMER_RIGHT_9_END_STABILITY=8.5000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=3.00 PRIMER_PAIR_9_PRODUCT_SIZE=2551 PRIMER_PAIR_10_PENALTY=183.964593 PRIMER_LEFT_10_PENALTY=181.705510 PRIMER_RIGHT_10_PENALTY=2.259083 PRIMER_LEFT_10_SEQUENCE=GCAGCTGCAAAGCATCTAAT PRIMER_RIGHT_10_SEQUENCE=TACAGCATGCCAAATCCTTT PRIMER_LEFT_10=9,20 PRIMER_RIGHT_10=2557,20 PRIMER_LEFT_10_TM=58.294 PRIMER_RIGHT_10_TM=58.241 PRIMER_LEFT_10_GC_PERCENT=45.000 PRIMER_RIGHT_10_GC_PERCENT=40.000 PRIMER_LEFT_10_SELF_ANY=8.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=3.00 PRIMER_LEFT_10_POSITION_PENALTY=180.000000 PRIMER_RIGHT_10_POSITION_PENALTY=0.500000 PRIMER_LEFT_10_END_STABILITY=5.9000 PRIMER_RIGHT_10_END_STABILITY=8.5000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PAIR_10_PRODUCT_SIZE=2549 PRIMER_PAIR_11_PENALTY=184.105424 PRIMER_LEFT_11_PENALTY=181.705510 PRIMER_RIGHT_11_PENALTY=2.399914 PRIMER_LEFT_11_SEQUENCE=GCAGCTGCAAAGCATCTAAT PRIMER_RIGHT_11_SEQUENCE=TTACAGCATGCCAAATCCTTT PRIMER_LEFT_11=9,20 PRIMER_RIGHT_11=2558,21 PRIMER_LEFT_11_TM=58.294 PRIMER_RIGHT_11_TM=59.600 PRIMER_LEFT_11_GC_PERCENT=45.000 PRIMER_RIGHT_11_GC_PERCENT=38.095 PRIMER_LEFT_11_SELF_ANY=8.00 PRIMER_RIGHT_11_SELF_ANY=6.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=3.00 PRIMER_LEFT_11_POSITION_PENALTY=180.000000 PRIMER_RIGHT_11_POSITION_PENALTY=1.000000 PRIMER_LEFT_11_END_STABILITY=5.9000 PRIMER_RIGHT_11_END_STABILITY=8.5000 PRIMER_PAIR_11_COMPL_ANY=6.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PAIR_11_PRODUCT_SIZE=2550 PRIMER_PAIR_12_PENALTY=184.464593 PRIMER_LEFT_12_PENALTY=181.705510 PRIMER_RIGHT_12_PENALTY=2.759083 PRIMER_LEFT_12_SEQUENCE=GCAGCTGCAAAGCATCTAAT PRIMER_RIGHT_12_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_12=9,20 PRIMER_RIGHT_12=2558,20 PRIMER_LEFT_12_TM=58.294 PRIMER_RIGHT_12_TM=58.241 PRIMER_LEFT_12_GC_PERCENT=45.000 PRIMER_RIGHT_12_GC_PERCENT=40.000 PRIMER_LEFT_12_SELF_ANY=8.00 PRIMER_RIGHT_12_SELF_ANY=6.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=1.00 PRIMER_LEFT_12_POSITION_PENALTY=180.000000 PRIMER_RIGHT_12_POSITION_PENALTY=1.000000 PRIMER_LEFT_12_END_STABILITY=5.9000 PRIMER_RIGHT_12_END_STABILITY=8.2000 PRIMER_PAIR_12_COMPL_ANY=6.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PAIR_12_PRODUCT_SIZE=2550 PRIMER_PAIR_13_PENALTY=184.546477 PRIMER_LEFT_13_PENALTY=181.705510 PRIMER_RIGHT_13_PENALTY=2.840968 PRIMER_LEFT_13_SEQUENCE=GCAGCTGCAAAGCATCTAAT PRIMER_RIGHT_13_SEQUENCE=ACAGCATGCCAAATCCTTT PRIMER_LEFT_13=9,20 PRIMER_RIGHT_13=2556,19 PRIMER_LEFT_13_TM=58.294 PRIMER_RIGHT_13_TM=58.159 PRIMER_LEFT_13_GC_PERCENT=45.000 PRIMER_RIGHT_13_GC_PERCENT=42.105 PRIMER_LEFT_13_SELF_ANY=8.00 PRIMER_RIGHT_13_SELF_ANY=6.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=3.00 PRIMER_LEFT_13_POSITION_PENALTY=180.000000 PRIMER_RIGHT_13_POSITION_PENALTY=0.000000 PRIMER_LEFT_13_END_STABILITY=5.9000 PRIMER_RIGHT_13_END_STABILITY=8.5000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PAIR_13_PRODUCT_SIZE=2548 PRIMER_PAIR_14_PENALTY=184.890860 PRIMER_LEFT_14_PENALTY=182.631776 PRIMER_RIGHT_14_PENALTY=2.259083 PRIMER_LEFT_14_SEQUENCE=GCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_14_SEQUENCE=TACAGCATGCCAAATCCTTT PRIMER_LEFT_14=9,21 PRIMER_RIGHT_14=2557,20 PRIMER_LEFT_14_TM=58.368 PRIMER_RIGHT_14_TM=58.241 PRIMER_LEFT_14_GC_PERCENT=42.857 PRIMER_RIGHT_14_GC_PERCENT=40.000 PRIMER_LEFT_14_SELF_ANY=8.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=3.00 PRIMER_RIGHT_14_SELF_END=3.00 PRIMER_LEFT_14_POSITION_PENALTY=180.000000 PRIMER_RIGHT_14_POSITION_PENALTY=0.500000 PRIMER_LEFT_14_END_STABILITY=5.2000 PRIMER_RIGHT_14_END_STABILITY=8.5000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=3.00 PRIMER_PAIR_14_PRODUCT_SIZE=2549 PRIMER_PAIR_15_PENALTY=185.031690 PRIMER_LEFT_15_PENALTY=182.631776 PRIMER_RIGHT_15_PENALTY=2.399914 PRIMER_LEFT_15_SEQUENCE=GCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_15_SEQUENCE=TTACAGCATGCCAAATCCTTT PRIMER_LEFT_15=9,21 PRIMER_RIGHT_15=2558,21 PRIMER_LEFT_15_TM=58.368 PRIMER_RIGHT_15_TM=59.600 PRIMER_LEFT_15_GC_PERCENT=42.857 PRIMER_RIGHT_15_GC_PERCENT=38.095 PRIMER_LEFT_15_SELF_ANY=8.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=3.00 PRIMER_RIGHT_15_SELF_END=3.00 PRIMER_LEFT_15_POSITION_PENALTY=180.000000 PRIMER_RIGHT_15_POSITION_PENALTY=1.000000 PRIMER_LEFT_15_END_STABILITY=5.2000 PRIMER_RIGHT_15_END_STABILITY=8.5000 PRIMER_PAIR_15_COMPL_ANY=6.00 PRIMER_PAIR_15_COMPL_END=3.00 PRIMER_PAIR_15_PRODUCT_SIZE=2550 PRIMER_PAIR_16_PENALTY=185.355825 PRIMER_LEFT_16_PENALTY=183.096742 PRIMER_RIGHT_16_PENALTY=2.259083 PRIMER_LEFT_16_SEQUENCE=GCAGCTGCAAAGCATCTAA PRIMER_RIGHT_16_SEQUENCE=TACAGCATGCCAAATCCTTT PRIMER_LEFT_16=9,19 PRIMER_RIGHT_16=2557,20 PRIMER_LEFT_16_TM=57.903 PRIMER_RIGHT_16_TM=58.241 PRIMER_LEFT_16_GC_PERCENT=47.368 PRIMER_RIGHT_16_GC_PERCENT=40.000 PRIMER_LEFT_16_SELF_ANY=8.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=1.00 PRIMER_RIGHT_16_SELF_END=3.00 PRIMER_LEFT_16_POSITION_PENALTY=180.000000 PRIMER_RIGHT_16_POSITION_PENALTY=0.500000 PRIMER_LEFT_16_END_STABILITY=6.0000 PRIMER_RIGHT_16_END_STABILITY=8.5000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=3.00 PRIMER_PAIR_16_PRODUCT_SIZE=2549 PRIMER_PAIR_17_PENALTY=185.390860 PRIMER_LEFT_17_PENALTY=182.631776 PRIMER_RIGHT_17_PENALTY=2.759083 PRIMER_LEFT_17_SEQUENCE=GCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_17_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_17=9,21 PRIMER_RIGHT_17=2558,20 PRIMER_LEFT_17_TM=58.368 PRIMER_RIGHT_17_TM=58.241 PRIMER_LEFT_17_GC_PERCENT=42.857 PRIMER_RIGHT_17_GC_PERCENT=40.000 PRIMER_LEFT_17_SELF_ANY=8.00 PRIMER_RIGHT_17_SELF_ANY=6.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=1.00 PRIMER_LEFT_17_POSITION_PENALTY=180.000000 PRIMER_RIGHT_17_POSITION_PENALTY=1.000000 PRIMER_LEFT_17_END_STABILITY=5.2000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=6.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PAIR_17_PRODUCT_SIZE=2550 PRIMER_PAIR_18_PENALTY=185.472744 PRIMER_LEFT_18_PENALTY=182.631776 PRIMER_RIGHT_18_PENALTY=2.840968 PRIMER_LEFT_18_SEQUENCE=GCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_18_SEQUENCE=ACAGCATGCCAAATCCTTT PRIMER_LEFT_18=9,21 PRIMER_RIGHT_18=2556,19 PRIMER_LEFT_18_TM=58.368 PRIMER_RIGHT_18_TM=58.159 PRIMER_LEFT_18_GC_PERCENT=42.857 PRIMER_RIGHT_18_GC_PERCENT=42.105 PRIMER_LEFT_18_SELF_ANY=8.00 PRIMER_RIGHT_18_SELF_ANY=6.00 PRIMER_LEFT_18_SELF_END=3.00 PRIMER_RIGHT_18_SELF_END=3.00 PRIMER_LEFT_18_POSITION_PENALTY=180.000000 PRIMER_RIGHT_18_POSITION_PENALTY=0.000000 PRIMER_LEFT_18_END_STABILITY=5.2000 PRIMER_RIGHT_18_END_STABILITY=8.5000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=3.00 PRIMER_PAIR_18_PRODUCT_SIZE=2548 PRIMER_PAIR_19_PENALTY=185.496656 PRIMER_LEFT_19_PENALTY=183.096742 PRIMER_RIGHT_19_PENALTY=2.399914 PRIMER_LEFT_19_SEQUENCE=GCAGCTGCAAAGCATCTAA PRIMER_RIGHT_19_SEQUENCE=TTACAGCATGCCAAATCCTTT PRIMER_LEFT_19=9,19 PRIMER_RIGHT_19=2558,21 PRIMER_LEFT_19_TM=57.903 PRIMER_RIGHT_19_TM=59.600 PRIMER_LEFT_19_GC_PERCENT=47.368 PRIMER_RIGHT_19_GC_PERCENT=38.095 PRIMER_LEFT_19_SELF_ANY=8.00 PRIMER_RIGHT_19_SELF_ANY=6.00 PRIMER_LEFT_19_SELF_END=1.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_POSITION_PENALTY=180.000000 PRIMER_RIGHT_19_POSITION_PENALTY=1.000000 PRIMER_LEFT_19_END_STABILITY=6.0000 PRIMER_RIGHT_19_END_STABILITY=8.5000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PAIR_19_PRODUCT_SIZE=2550 = SEQUENCE_ID=L22005/HCDC34 P3_COMMENT=Human ubiquitin conjugating enzyme mRNA, partial cds. CDS_<1..897 SEQUENCE_START_CODON_POSITION=-3 SEQUENCE_TEMPLATE=ATTGCGGCGGCGCCAGAGCTGCTGGAGCGCTCGGGGTCCCCGGGCGGCGGCGGCGGCGCAGAGGAGGAGGCAGGCGGCGGCCCCGGTGGCTCCCCCCCGGACGGTGCGCGGCCCGGCCCGTCTCGCGAACTCGCGGTGGTCGCGCGGCCCCGCGCTGCTCCGACCCCGGGCCCCTCCGCCGCCGCCATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATTTTCGGGCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCCCACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACGAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCCGACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGAGGAGTCCTGACACCACCAGAATAAACTTGCCGAGTTTACCTCACTAGGGCCGGACCCGTGGCTCCTTAGACGACAGACTACCTCACGGAGGTTTTGTGCTGGTCCCCGTCTCCTCTGGTTGTTTCGTTTTGGCTTTTTCTCCCTCCCCATGTCTGTTCTGGGTTTTCACGTGCTTCAGAGAAGAGGGGCTGCCCCACCGCCACTCACGTCACTCGGGGCTCGGTGGACGGGCCCAGGGTGGGAGCGGCCGGCCCACCTGTCCCCTCGGGAGGGGAGCTGAGCCCGACTTCTACCGGGGTCCCCCAGCTTCCGGACTGGCCGCACCCCGGAGGAGCCACGGGGGCGCTGCTGGGAACGTGGGCGGGGGGCCGTTTCCTGACACTACCAGCCTGGGAGGCCCAGGTGTAGCGGTCCGAGGGGCCCGGTCCTGCCTGTCAGCTCCAGGTCCTGGAGCCACGTCCAGCACTGAGTGGACGGATTCACCAAT PRIMER_LEFT_EXPLAIN=considered 11417, would not amplify any of the ORF 8815, GC content failed 428, low tm 104, high tm 1651, high end compl 2, long poly-x seq 18, high 3' stability 24, ok 375 PRIMER_RIGHT_EXPLAIN=considered 11034, GC content failed 1402, low tm 461, high tm 7364, high end compl 5, long poly-x seq 44, high 3' stability 50, ok 1708 PRIMER_PAIR_EXPLAIN=considered 23, high end compl 2, ok 21 PRIMER_STOP_CODON_POSITION=894 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=4027.917651 PRIMER_LEFT_0_PENALTY=4023.813185 PRIMER_RIGHT_0_PENALTY=4.104466 PRIMER_LEFT_0_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_0_SEQUENCE=GGTGTCAGGACTCCTCCGT PRIMER_LEFT_0=198,19 PRIMER_RIGHT_0=900,19 PRIMER_LEFT_0_TM=62.813 PRIMER_RIGHT_0_TM=60.104 PRIMER_LEFT_0_GC_PERCENT=63.158 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_0_POSITION_PENALTY=3.000000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=9.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=703 PRIMER_PAIR_1_PENALTY=4028.102281 PRIMER_LEFT_1_PENALTY=4023.813185 PRIMER_RIGHT_1_PENALTY=4.289096 PRIMER_LEFT_1_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_1_SEQUENCE=GTGTCAGGACTCCTCCGTG PRIMER_LEFT_1=198,19 PRIMER_RIGHT_1=899,19 PRIMER_LEFT_1_TM=62.813 PRIMER_RIGHT_1_TM=59.211 PRIMER_LEFT_1_GC_PERCENT=63.158 PRIMER_RIGHT_1_GC_PERCENT=63.158 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_1_POSITION_PENALTY=2.500000 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=9.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=702 PRIMER_PAIR_2_PENALTY=4028.107106 PRIMER_LEFT_2_PENALTY=4024.002640 PRIMER_RIGHT_2_PENALTY=4.104466 PRIMER_LEFT_2_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_2_SEQUENCE=GGTGTCAGGACTCCTCCGT PRIMER_LEFT_2=198,20 PRIMER_RIGHT_2=900,19 PRIMER_LEFT_2_TM=64.003 PRIMER_RIGHT_2_TM=60.104 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=63.158 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_2_POSITION_PENALTY=3.000000 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=9.6000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=703 PRIMER_PAIR_3_PENALTY=4028.179180 PRIMER_LEFT_3_PENALTY=4023.813185 PRIMER_RIGHT_3_PENALTY=4.365996 PRIMER_LEFT_3_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_3_SEQUENCE=AGGACTCCTCCGTGCCAGA PRIMER_LEFT_3=198,19 PRIMER_RIGHT_3=894,19 PRIMER_LEFT_3_TM=62.813 PRIMER_RIGHT_3_TM=63.366 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=63.158 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.000000 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=697 PRIMER_PAIR_4_PENALTY=4028.291736 PRIMER_LEFT_4_PENALTY=4024.002640 PRIMER_RIGHT_4_PENALTY=4.289096 PRIMER_LEFT_4_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_4_SEQUENCE=GTGTCAGGACTCCTCCGTG PRIMER_LEFT_4=198,20 PRIMER_RIGHT_4=899,19 PRIMER_LEFT_4_TM=64.003 PRIMER_RIGHT_4_TM=59.211 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=63.158 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_4_POSITION_PENALTY=2.500000 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=9.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=702 PRIMER_PAIR_5_PENALTY=4028.368636 PRIMER_LEFT_5_PENALTY=4024.002640 PRIMER_RIGHT_5_PENALTY=4.365996 PRIMER_LEFT_5_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_5_SEQUENCE=AGGACTCCTCCGTGCCAGA PRIMER_LEFT_5=198,20 PRIMER_RIGHT_5=894,19 PRIMER_LEFT_5_TM=64.003 PRIMER_RIGHT_5_TM=63.366 PRIMER_LEFT_5_GC_PERCENT=60.000 PRIMER_RIGHT_5_GC_PERCENT=63.158 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=7.00 PRIMER_LEFT_5_SELF_END=2.00 PRIMER_RIGHT_5_SELF_END=2.00 PRIMER_LEFT_5_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_5_POSITION_PENALTY=0.000000 PRIMER_LEFT_5_END_STABILITY=7.0000 PRIMER_RIGHT_5_END_STABILITY=8.2000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PAIR_5_PRODUCT_SIZE=697 PRIMER_PAIR_6_PENALTY=4029.302304 PRIMER_LEFT_6_PENALTY=4023.813185 PRIMER_RIGHT_6_PENALTY=5.489119 PRIMER_LEFT_6_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_6_SEQUENCE=TGTCAGGACTCCTCCGTGC PRIMER_LEFT_6=198,19 PRIMER_RIGHT_6=898,19 PRIMER_LEFT_6_TM=62.813 PRIMER_RIGHT_6_TM=62.489 PRIMER_LEFT_6_GC_PERCENT=63.158 PRIMER_RIGHT_6_GC_PERCENT=63.158 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=7.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_6_POSITION_PENALTY=2.000000 PRIMER_LEFT_6_END_STABILITY=8.2000 PRIMER_RIGHT_6_END_STABILITY=9.9000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=701 PRIMER_PAIR_7_PENALTY=4029.563238 PRIMER_LEFT_7_PENALTY=4023.813185 PRIMER_RIGHT_7_PENALTY=5.750053 PRIMER_LEFT_7_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_7_SEQUENCE=GTCAGGACTCCTCCGTGCC PRIMER_LEFT_7=198,19 PRIMER_RIGHT_7=897,19 PRIMER_LEFT_7_TM=62.813 PRIMER_RIGHT_7_TM=63.250 PRIMER_LEFT_7_GC_PERCENT=63.158 PRIMER_RIGHT_7_GC_PERCENT=68.421 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=7.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_7_POSITION_PENALTY=1.500000 PRIMER_LEFT_7_END_STABILITY=8.2000 PRIMER_RIGHT_7_END_STABILITY=9.4000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PAIR_7_PRODUCT_SIZE=700 PRIMER_PAIR_8_PENALTY=4029.590879 PRIMER_LEFT_8_PENALTY=4023.813185 PRIMER_RIGHT_8_PENALTY=5.777694 PRIMER_LEFT_8_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_8_SEQUENCE=GTGTCAGGACTCCTCCGTGC PRIMER_LEFT_8=198,19 PRIMER_RIGHT_8=899,20 PRIMER_LEFT_8_TM=62.813 PRIMER_RIGHT_8_TM=63.278 PRIMER_LEFT_8_GC_PERCENT=63.158 PRIMER_RIGHT_8_GC_PERCENT=65.000 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=7.00 PRIMER_LEFT_8_SELF_END=2.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_8_POSITION_PENALTY=2.500000 PRIMER_LEFT_8_END_STABILITY=8.2000 PRIMER_RIGHT_8_END_STABILITY=9.9000 PRIMER_PAIR_8_COMPL_ANY=4.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PAIR_8_PRODUCT_SIZE=702 PRIMER_PAIR_9_PENALTY=4029.657883 PRIMER_LEFT_9_PENALTY=4023.813185 PRIMER_RIGHT_9_PENALTY=5.844698 PRIMER_LEFT_9_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_9_SEQUENCE=AGGACTCCTCCGTGCCAGAGT PRIMER_LEFT_9=198,19 PRIMER_RIGHT_9=894,21 PRIMER_LEFT_9_TM=62.813 PRIMER_RIGHT_9_TM=64.845 PRIMER_LEFT_9_GC_PERCENT=63.158 PRIMER_RIGHT_9_GC_PERCENT=61.905 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=7.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_9_POSITION_PENALTY=0.000000 PRIMER_LEFT_9_END_STABILITY=8.2000 PRIMER_RIGHT_9_END_STABILITY=6.1000 PRIMER_PAIR_9_COMPL_ANY=4.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=697 PRIMER_PAIR_10_PENALTY=4029.703903 PRIMER_LEFT_10_PENALTY=4023.813185 PRIMER_RIGHT_10_PENALTY=5.890719 PRIMER_LEFT_10_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_10_SEQUENCE=CAGGACTCCTCCGTGCCAG PRIMER_LEFT_10=198,19 PRIMER_RIGHT_10=895,19 PRIMER_LEFT_10_TM=62.813 PRIMER_RIGHT_10_TM=64.391 PRIMER_LEFT_10_GC_PERCENT=63.158 PRIMER_RIGHT_10_GC_PERCENT=68.421 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=7.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_10_POSITION_PENALTY=0.500000 PRIMER_LEFT_10_END_STABILITY=8.2000 PRIMER_RIGHT_10_END_STABILITY=9.7000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PAIR_10_PRODUCT_SIZE=698 PRIMER_PAIR_11_PENALTY=4029.752693 PRIMER_LEFT_11_PENALTY=4024.002640 PRIMER_RIGHT_11_PENALTY=5.750053 PRIMER_LEFT_11_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_11_SEQUENCE=GTCAGGACTCCTCCGTGCC PRIMER_LEFT_11=198,20 PRIMER_RIGHT_11=897,19 PRIMER_LEFT_11_TM=64.003 PRIMER_RIGHT_11_TM=63.250 PRIMER_LEFT_11_GC_PERCENT=60.000 PRIMER_RIGHT_11_GC_PERCENT=68.421 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=7.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_11_POSITION_PENALTY=1.500000 PRIMER_LEFT_11_END_STABILITY=7.0000 PRIMER_RIGHT_11_END_STABILITY=9.4000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=3.00 PRIMER_PAIR_11_PRODUCT_SIZE=700 PRIMER_PAIR_12_PENALTY=4029.847338 PRIMER_LEFT_12_PENALTY=4024.002640 PRIMER_RIGHT_12_PENALTY=5.844698 PRIMER_LEFT_12_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_12_SEQUENCE=AGGACTCCTCCGTGCCAGAGT PRIMER_LEFT_12=198,20 PRIMER_RIGHT_12=894,21 PRIMER_LEFT_12_TM=64.003 PRIMER_RIGHT_12_TM=64.845 PRIMER_LEFT_12_GC_PERCENT=60.000 PRIMER_RIGHT_12_GC_PERCENT=61.905 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=7.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_12_POSITION_PENALTY=0.000000 PRIMER_LEFT_12_END_STABILITY=7.0000 PRIMER_RIGHT_12_END_STABILITY=6.1000 PRIMER_PAIR_12_COMPL_ANY=4.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PAIR_12_PRODUCT_SIZE=697 PRIMER_PAIR_13_PENALTY=4029.893359 PRIMER_LEFT_13_PENALTY=4024.002640 PRIMER_RIGHT_13_PENALTY=5.890719 PRIMER_LEFT_13_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_13_SEQUENCE=CAGGACTCCTCCGTGCCAG PRIMER_LEFT_13=198,20 PRIMER_RIGHT_13=895,19 PRIMER_LEFT_13_TM=64.003 PRIMER_RIGHT_13_TM=64.391 PRIMER_LEFT_13_GC_PERCENT=60.000 PRIMER_RIGHT_13_GC_PERCENT=68.421 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_LEFT_13_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_13_POSITION_PENALTY=0.500000 PRIMER_LEFT_13_END_STABILITY=7.0000 PRIMER_RIGHT_13_END_STABILITY=9.7000 PRIMER_PAIR_13_COMPL_ANY=4.00 PRIMER_PAIR_13_COMPL_END=3.00 PRIMER_PAIR_13_PRODUCT_SIZE=698 PRIMER_PAIR_14_PENALTY=4029.932546 PRIMER_LEFT_14_PENALTY=4023.813185 PRIMER_RIGHT_14_PENALTY=6.119361 PRIMER_LEFT_14_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_14_SEQUENCE=GGTGTCAGGACTCCTCCGTG PRIMER_LEFT_14=198,19 PRIMER_RIGHT_14=900,20 PRIMER_LEFT_14_TM=62.813 PRIMER_RIGHT_14_TM=63.119 PRIMER_LEFT_14_GC_PERCENT=63.158 PRIMER_RIGHT_14_GC_PERCENT=65.000 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=7.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_14_POSITION_PENALTY=3.000000 PRIMER_LEFT_14_END_STABILITY=8.2000 PRIMER_RIGHT_14_END_STABILITY=9.9000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PAIR_14_PRODUCT_SIZE=703 PRIMER_PAIR_15_PENALTY=4030.122001 PRIMER_LEFT_15_PENALTY=4024.002640 PRIMER_RIGHT_15_PENALTY=6.119361 PRIMER_LEFT_15_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_15_SEQUENCE=GGTGTCAGGACTCCTCCGTG PRIMER_LEFT_15=198,20 PRIMER_RIGHT_15=900,20 PRIMER_LEFT_15_TM=64.003 PRIMER_RIGHT_15_TM=63.119 PRIMER_LEFT_15_GC_PERCENT=60.000 PRIMER_RIGHT_15_GC_PERCENT=65.000 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=7.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_15_POSITION_PENALTY=3.000000 PRIMER_LEFT_15_END_STABILITY=7.0000 PRIMER_RIGHT_15_END_STABILITY=9.9000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=3.00 PRIMER_PAIR_15_PRODUCT_SIZE=703 PRIMER_PAIR_16_PENALTY=4030.432546 PRIMER_LEFT_16_PENALTY=4023.813185 PRIMER_RIGHT_16_PENALTY=6.619361 PRIMER_LEFT_16_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_16_SEQUENCE=TGGTGTCAGGACTCCTCCGT PRIMER_LEFT_16=198,19 PRIMER_RIGHT_16=901,20 PRIMER_LEFT_16_TM=62.813 PRIMER_RIGHT_16_TM=63.119 PRIMER_LEFT_16_GC_PERCENT=63.158 PRIMER_RIGHT_16_GC_PERCENT=60.000 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=7.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_16_POSITION_PENALTY=3.500000 PRIMER_LEFT_16_END_STABILITY=8.2000 PRIMER_RIGHT_16_END_STABILITY=9.6000 PRIMER_PAIR_16_COMPL_ANY=3.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PAIR_16_PRODUCT_SIZE=704 PRIMER_PAIR_17_PENALTY=4030.622001 PRIMER_LEFT_17_PENALTY=4024.002640 PRIMER_RIGHT_17_PENALTY=6.619361 PRIMER_LEFT_17_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_17_SEQUENCE=TGGTGTCAGGACTCCTCCGT PRIMER_LEFT_17=198,20 PRIMER_RIGHT_17=901,20 PRIMER_LEFT_17_TM=64.003 PRIMER_RIGHT_17_TM=63.119 PRIMER_LEFT_17_GC_PERCENT=60.000 PRIMER_RIGHT_17_GC_PERCENT=60.000 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=7.00 PRIMER_LEFT_17_SELF_END=2.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_17_POSITION_PENALTY=3.500000 PRIMER_LEFT_17_END_STABILITY=7.0000 PRIMER_RIGHT_17_END_STABILITY=9.6000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PAIR_17_PRODUCT_SIZE=704 PRIMER_PAIR_18_PENALTY=4030.636711 PRIMER_LEFT_18_PENALTY=4023.813185 PRIMER_RIGHT_18_PENALTY=6.823526 PRIMER_LEFT_18_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_18_SEQUENCE=TGGTGTCAGGACTCCTCCG PRIMER_LEFT_18=198,19 PRIMER_RIGHT_18=901,19 PRIMER_LEFT_18_TM=62.813 PRIMER_RIGHT_18_TM=62.324 PRIMER_LEFT_18_GC_PERCENT=63.158 PRIMER_RIGHT_18_GC_PERCENT=63.158 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=7.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_18_POSITION_PENALTY=3.500000 PRIMER_LEFT_18_END_STABILITY=8.2000 PRIMER_RIGHT_18_END_STABILITY=9.9000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PAIR_18_PRODUCT_SIZE=704 PRIMER_PAIR_19_PENALTY=4030.826166 PRIMER_LEFT_19_PENALTY=4024.002640 PRIMER_RIGHT_19_PENALTY=6.823526 PRIMER_LEFT_19_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_19_SEQUENCE=TGGTGTCAGGACTCCTCCG PRIMER_LEFT_19=198,20 PRIMER_RIGHT_19=901,19 PRIMER_LEFT_19_TM=64.003 PRIMER_RIGHT_19_TM=62.324 PRIMER_LEFT_19_GC_PERCENT=60.000 PRIMER_RIGHT_19_GC_PERCENT=63.158 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=7.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_19_POSITION_PENALTY=3.500000 PRIMER_LEFT_19_END_STABILITY=7.0000 PRIMER_RIGHT_19_END_STABILITY=9.9000 PRIMER_PAIR_19_COMPL_ANY=3.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PAIR_19_PRODUCT_SIZE=704 = SEQUENCE_ID=M24194/MHC/RACK1 P3_COMMENT=Human MHC protein homologous to chicken B complex protein mRNA, complete cds. CDS_96..1049 SEQUENCE_START_CODON_POSITION=95 SEQUENCE_TEMPLATE=CTGCAAGGCGGCGGCAGGAGAGGTTGTGGTGCTAGTTTCTCTAAGCCATCCAGTGCCATCCTCGTCGCTGCAGCGACACCGCTCTCGCCGCCGCCATGACTGAGCAGATGACCCTTCGTGGCACCCTCAAGGGCCACAACGGCTGGGTAACCCAGATCGCTACTACCCCGCAGTTCCCGGACATGATCCTCTCCGCCTCTCGAGATAAGACCATCATCATGTGGAAACTGACCAGGGATGAGACCAACTATGGAATTCCACAGCGTGCTCTGCGGGGTCACTCCCACTTTGTTAGTGATGTGGTTATCTCCTCAGATGGCCAGTTTGCCCTCTCAGGCTCCTGGGATGGAACCCTGCGCCTCTGGGATCTCACAACGGGCACCACCACGAGGCGATTTGTGGGCCATACCAAGGATGTGCTGAGTGTGGCCTTCTCCTCTGACAACCGGCAGATTGTCTCTGGATCTCGAGATAAAACCATCAAGCTATGGAATACCCTGGGTGTGTGCAAATACACTGTCCAGGATGAGAGCCACTCAGAGTGGGTGTCTTGTGTCCGCTTCTCGCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTGGCTAACTGCAAGCTGAAGACCAACCACATTGGCCACACAGGCTATCTGAACACGGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGAGGCAAGGATGGCCAGGCCATGTTATGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACATCATCAACGCCCTGTGCTTCAGCCCTAACCGCTACTGGCTGTGTGCTGCCACAGGCCCCAGCATCAAGATCTGGGATTTAGAGGGAAAGATCATTGTAGATGAACTGAAGCAAGAAGTTATCAGTACCAGCAGCAAGGCAGAACCACCCCAGTGCACTTCCCTGGCCTGGTCTGCTGATGGCCAGACTCTGTTTGCTGGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAAAAAATGGCTTTTC PRIMER_LEFT_EXPLAIN=considered 8997, would not amplify any of the ORF 6119, GC content failed 1, low tm 301, high tm 1735, high 3' stability 24, ok 817 PRIMER_RIGHT_EXPLAIN=considered 8758, GC content failed 64, low tm 957, high tm 5153, high end compl 7, long poly-x seq 16, high 3' stability 45, ok 2516 PRIMER_PAIR_EXPLAIN=considered 38, high end compl 14, ok 24 PRIMER_STOP_CODON_POSITION=1046 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=5.438070 PRIMER_LEFT_0_PENALTY=2.658657 PRIMER_RIGHT_0_PENALTY=2.779412 PRIMER_LEFT_0_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_0_SEQUENCE=TCTAGCGTGTGCCAATGGT PRIMER_LEFT_0=95,22 PRIMER_RIGHT_0=1049,19 PRIMER_LEFT_0_TM=60.659 PRIMER_RIGHT_0_TM=60.279 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=52.632 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=1.500000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=7.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=955 PRIMER_PAIR_1_PENALTY=5.568198 PRIMER_LEFT_1_PENALTY=2.788786 PRIMER_RIGHT_1_PENALTY=2.779412 PRIMER_LEFT_1_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_1_SEQUENCE=TCTAGCGTGTGCCAATGGT PRIMER_LEFT_1=95,20 PRIMER_RIGHT_1=1049,19 PRIMER_LEFT_1_TM=57.211 PRIMER_RIGHT_1_TM=60.279 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=52.632 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=1.500000 PRIMER_LEFT_1_END_STABILITY=9.1000 PRIMER_RIGHT_1_END_STABILITY=7.8000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=955 PRIMER_PAIR_2_PENALTY=6.289329 PRIMER_LEFT_2_PENALTY=2.658657 PRIMER_RIGHT_2_PENALTY=3.630671 PRIMER_LEFT_2_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_2_SEQUENCE=TTCTAGCGTGTGCCAATGGT PRIMER_LEFT_2=95,22 PRIMER_RIGHT_2=1050,20 PRIMER_LEFT_2_TM=60.659 PRIMER_RIGHT_2_TM=61.631 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=2.000000 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=7.8000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=956 PRIMER_PAIR_3_PENALTY=6.289706 PRIMER_LEFT_3_PENALTY=2.241606 PRIMER_RIGHT_3_PENALTY=4.048100 PRIMER_LEFT_3_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_3_SEQUENCE=ACTTCTAGCGTGTGCCAATG PRIMER_LEFT_3=95,21 PRIMER_RIGHT_3=1052,20 PRIMER_LEFT_3_TM=58.758 PRIMER_RIGHT_3_TM=58.952 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=3.000000 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=958 PRIMER_PAIR_4_PENALTY=6.419457 PRIMER_LEFT_4_PENALTY=2.788786 PRIMER_RIGHT_4_PENALTY=3.630671 PRIMER_LEFT_4_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_4_SEQUENCE=TTCTAGCGTGTGCCAATGGT PRIMER_LEFT_4=95,20 PRIMER_RIGHT_4=1050,20 PRIMER_LEFT_4_TM=57.211 PRIMER_RIGHT_4_TM=61.631 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=2.000000 PRIMER_LEFT_4_END_STABILITY=9.1000 PRIMER_RIGHT_4_END_STABILITY=7.8000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=956 PRIMER_PAIR_5_PENALTY=6.706757 PRIMER_LEFT_5_PENALTY=2.658657 PRIMER_RIGHT_5_PENALTY=4.048100 PRIMER_LEFT_5_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_5_SEQUENCE=ACTTCTAGCGTGTGCCAATG PRIMER_LEFT_5=95,22 PRIMER_RIGHT_5=1052,20 PRIMER_LEFT_5_TM=60.659 PRIMER_RIGHT_5_TM=58.952 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=50.000 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=3.000000 PRIMER_LEFT_5_END_STABILITY=8.2000 PRIMER_RIGHT_5_END_STABILITY=7.2000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=958 PRIMER_PAIR_6_PENALTY=6.836886 PRIMER_LEFT_6_PENALTY=2.788786 PRIMER_RIGHT_6_PENALTY=4.048100 PRIMER_LEFT_6_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_6_SEQUENCE=ACTTCTAGCGTGTGCCAATG PRIMER_LEFT_6=95,20 PRIMER_RIGHT_6=1052,20 PRIMER_LEFT_6_TM=57.211 PRIMER_RIGHT_6_TM=58.952 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=4.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=3.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=3.000000 PRIMER_LEFT_6_END_STABILITY=9.1000 PRIMER_RIGHT_6_END_STABILITY=7.2000 PRIMER_PAIR_6_COMPL_ANY=3.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PAIR_6_PRODUCT_SIZE=958 PRIMER_PAIR_7_PENALTY=7.061025 PRIMER_LEFT_7_PENALTY=2.241606 PRIMER_RIGHT_7_PENALTY=4.819419 PRIMER_LEFT_7_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_7_SEQUENCE=AACTTCTAGCGTGTGCCAATG PRIMER_LEFT_7=95,21 PRIMER_RIGHT_7=1053,21 PRIMER_LEFT_7_TM=58.758 PRIMER_RIGHT_7_TM=60.319 PRIMER_LEFT_7_GC_PERCENT=47.619 PRIMER_RIGHT_7_GC_PERCENT=47.619 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=3.500000 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=7.2000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PAIR_7_PRODUCT_SIZE=959 PRIMER_PAIR_8_PENALTY=7.478076 PRIMER_LEFT_8_PENALTY=2.658657 PRIMER_RIGHT_8_PENALTY=4.819419 PRIMER_LEFT_8_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_8_SEQUENCE=AACTTCTAGCGTGTGCCAATG PRIMER_LEFT_8=95,22 PRIMER_RIGHT_8=1053,21 PRIMER_LEFT_8_TM=60.659 PRIMER_RIGHT_8_TM=60.319 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=47.619 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=4.00 PRIMER_LEFT_8_SELF_END=1.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=3.500000 PRIMER_LEFT_8_END_STABILITY=8.2000 PRIMER_RIGHT_8_END_STABILITY=7.2000 PRIMER_PAIR_8_COMPL_ANY=3.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PAIR_8_PRODUCT_SIZE=959 PRIMER_PAIR_9_PENALTY=7.608205 PRIMER_LEFT_9_PENALTY=2.788786 PRIMER_RIGHT_9_PENALTY=4.819419 PRIMER_LEFT_9_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_9_SEQUENCE=AACTTCTAGCGTGTGCCAATG PRIMER_LEFT_9=95,20 PRIMER_RIGHT_9=1053,21 PRIMER_LEFT_9_TM=57.211 PRIMER_RIGHT_9_TM=60.319 PRIMER_LEFT_9_GC_PERCENT=50.000 PRIMER_RIGHT_9_GC_PERCENT=47.619 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=3.500000 PRIMER_LEFT_9_END_STABILITY=9.1000 PRIMER_RIGHT_9_END_STABILITY=7.2000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=959 PRIMER_PAIR_10_PENALTY=7.661525 PRIMER_LEFT_10_PENALTY=2.241606 PRIMER_RIGHT_10_PENALTY=5.419920 PRIMER_LEFT_10_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_10_SEQUENCE=AAACTTCTAGCGTGTGCCAA PRIMER_LEFT_10=95,21 PRIMER_RIGHT_10=1054,20 PRIMER_LEFT_10_TM=58.758 PRIMER_RIGHT_10_TM=58.580 PRIMER_LEFT_10_GC_PERCENT=47.619 PRIMER_RIGHT_10_GC_PERCENT=45.000 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=4.000000 PRIMER_LEFT_10_END_STABILITY=9.7000 PRIMER_RIGHT_10_END_STABILITY=10.0000 PRIMER_PAIR_10_COMPL_ANY=3.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PAIR_10_PRODUCT_SIZE=960 PRIMER_PAIR_11_PENALTY=7.728937 PRIMER_LEFT_11_PENALTY=2.241606 PRIMER_RIGHT_11_PENALTY=5.487331 PRIMER_LEFT_11_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_11_SEQUENCE=CTTCTAGCGTGTGCCAATG PRIMER_LEFT_11=95,21 PRIMER_RIGHT_11=1051,19 PRIMER_LEFT_11_TM=58.758 PRIMER_RIGHT_11_TM=58.013 PRIMER_LEFT_11_GC_PERCENT=47.619 PRIMER_RIGHT_11_GC_PERCENT=52.632 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=3.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=2.500000 PRIMER_LEFT_11_END_STABILITY=9.7000 PRIMER_RIGHT_11_END_STABILITY=7.2000 PRIMER_PAIR_11_COMPL_ANY=3.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PAIR_11_PRODUCT_SIZE=957 PRIMER_PAIR_12_PENALTY=8.078577 PRIMER_LEFT_12_PENALTY=2.658657 PRIMER_RIGHT_12_PENALTY=5.419920 PRIMER_LEFT_12_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_12_SEQUENCE=AAACTTCTAGCGTGTGCCAA PRIMER_LEFT_12=95,22 PRIMER_RIGHT_12=1054,20 PRIMER_LEFT_12_TM=60.659 PRIMER_RIGHT_12_TM=58.580 PRIMER_LEFT_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_GC_PERCENT=45.000 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=1.00 PRIMER_RIGHT_12_SELF_END=1.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=4.000000 PRIMER_LEFT_12_END_STABILITY=8.2000 PRIMER_RIGHT_12_END_STABILITY=10.0000 PRIMER_PAIR_12_COMPL_ANY=3.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PAIR_12_PRODUCT_SIZE=960 PRIMER_PAIR_13_PENALTY=8.145989 PRIMER_LEFT_13_PENALTY=2.658657 PRIMER_RIGHT_13_PENALTY=5.487331 PRIMER_LEFT_13_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_13_SEQUENCE=CTTCTAGCGTGTGCCAATG PRIMER_LEFT_13=95,22 PRIMER_RIGHT_13=1051,19 PRIMER_LEFT_13_TM=60.659 PRIMER_RIGHT_13_TM=58.013 PRIMER_LEFT_13_GC_PERCENT=50.000 PRIMER_RIGHT_13_GC_PERCENT=52.632 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=1.00 PRIMER_RIGHT_13_SELF_END=3.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=2.500000 PRIMER_LEFT_13_END_STABILITY=8.2000 PRIMER_RIGHT_13_END_STABILITY=7.2000 PRIMER_PAIR_13_COMPL_ANY=3.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PAIR_13_PRODUCT_SIZE=957 PRIMER_PAIR_14_PENALTY=8.208706 PRIMER_LEFT_14_PENALTY=2.788786 PRIMER_RIGHT_14_PENALTY=5.419920 PRIMER_LEFT_14_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_14_SEQUENCE=AAACTTCTAGCGTGTGCCAA PRIMER_LEFT_14=95,20 PRIMER_RIGHT_14=1054,20 PRIMER_LEFT_14_TM=57.211 PRIMER_RIGHT_14_TM=58.580 PRIMER_LEFT_14_GC_PERCENT=50.000 PRIMER_RIGHT_14_GC_PERCENT=45.000 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=4.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=4.000000 PRIMER_LEFT_14_END_STABILITY=9.1000 PRIMER_RIGHT_14_END_STABILITY=10.0000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=1.00 PRIMER_PAIR_14_PRODUCT_SIZE=960 PRIMER_PAIR_15_PENALTY=8.232043 PRIMER_LEFT_15_PENALTY=2.241606 PRIMER_RIGHT_15_PENALTY=5.990437 PRIMER_LEFT_15_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_15_SEQUENCE=AACTTCTAGCGTGTGCCAAT PRIMER_LEFT_15=95,21 PRIMER_RIGHT_15=1053,20 PRIMER_LEFT_15_TM=58.758 PRIMER_RIGHT_15_TM=57.510 PRIMER_LEFT_15_GC_PERCENT=47.619 PRIMER_RIGHT_15_GC_PERCENT=45.000 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=4.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=3.500000 PRIMER_LEFT_15_END_STABILITY=9.7000 PRIMER_RIGHT_15_END_STABILITY=8.4000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PAIR_15_PRODUCT_SIZE=959 PRIMER_PAIR_16_PENALTY=8.276117 PRIMER_LEFT_16_PENALTY=2.788786 PRIMER_RIGHT_16_PENALTY=5.487331 PRIMER_LEFT_16_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_16_SEQUENCE=CTTCTAGCGTGTGCCAATG PRIMER_LEFT_16=95,20 PRIMER_RIGHT_16=1051,19 PRIMER_LEFT_16_TM=57.211 PRIMER_RIGHT_16_TM=58.013 PRIMER_LEFT_16_GC_PERCENT=50.000 PRIMER_RIGHT_16_GC_PERCENT=52.632 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=3.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=2.500000 PRIMER_LEFT_16_END_STABILITY=9.1000 PRIMER_RIGHT_16_END_STABILITY=7.2000 PRIMER_PAIR_16_COMPL_ANY=3.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PAIR_16_PRODUCT_SIZE=957 PRIMER_PAIR_17_PENALTY=8.322092 PRIMER_LEFT_17_PENALTY=2.241606 PRIMER_RIGHT_17_PENALTY=6.080486 PRIMER_LEFT_17_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_17_SEQUENCE=AAACTTCTAGCGTGTGCCAAT PRIMER_LEFT_17=95,21 PRIMER_RIGHT_17=1054,21 PRIMER_LEFT_17_TM=58.758 PRIMER_RIGHT_17_TM=58.920 PRIMER_LEFT_17_GC_PERCENT=47.619 PRIMER_RIGHT_17_GC_PERCENT=42.857 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=4.000000 PRIMER_LEFT_17_END_STABILITY=9.7000 PRIMER_RIGHT_17_END_STABILITY=8.4000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PAIR_17_PRODUCT_SIZE=960 PRIMER_PAIR_18_PENALTY=8.649095 PRIMER_LEFT_18_PENALTY=2.658657 PRIMER_RIGHT_18_PENALTY=5.990437 PRIMER_LEFT_18_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_18_SEQUENCE=AACTTCTAGCGTGTGCCAAT PRIMER_LEFT_18=95,22 PRIMER_RIGHT_18=1053,20 PRIMER_LEFT_18_TM=60.659 PRIMER_RIGHT_18_TM=57.510 PRIMER_LEFT_18_GC_PERCENT=50.000 PRIMER_RIGHT_18_GC_PERCENT=45.000 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_LEFT_18_SELF_END=1.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=3.500000 PRIMER_LEFT_18_END_STABILITY=8.2000 PRIMER_RIGHT_18_END_STABILITY=8.4000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PAIR_18_PRODUCT_SIZE=959 PRIMER_PAIR_19_PENALTY=8.650935 PRIMER_LEFT_19_PENALTY=2.658657 PRIMER_RIGHT_19_PENALTY=5.992277 PRIMER_LEFT_19_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_19_SEQUENCE=CTTCTAGCGTGTGCCAATGGT PRIMER_LEFT_19=95,22 PRIMER_RIGHT_19=1051,21 PRIMER_LEFT_19_TM=60.659 PRIMER_RIGHT_19_TM=62.492 PRIMER_LEFT_19_GC_PERCENT=50.000 PRIMER_RIGHT_19_GC_PERCENT=52.381 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=5.00 PRIMER_LEFT_19_SELF_END=1.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=2.500000 PRIMER_LEFT_19_END_STABILITY=8.2000 PRIMER_RIGHT_19_END_STABILITY=7.8000 PRIMER_PAIR_19_COMPL_ANY=4.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PAIR_19_PRODUCT_SIZE=957 = SEQUENCE_ID=X66357/CDK3 P3_COMMENT=H.sapiens mRNA cdk3 for serine/threonine protein kinase. CDS_89..1006 SEQUENCE_START_CODON_POSITION=88 SEQUENCE_TEMPLATE=CCACATGGAAGCTGGAGGAGCAACCGGGAGCGCTGGGCTGGGGTGCAAATTGCCCAGTGCCTTCTGTTTCCCAGGCAGCTCTGTGGCCATGGATATGTTCCAGAAGGTAGAGAAGATCGGAGAGGGCACCTATGGGGTGGTGTACAAGGCCAAGAACAGGGAGACAGGGCAGCTGGTGGCCCTGAAGAAGATCAGACTGGATTTGGAGATGGAGGGGGTCCCAAGCACTGCCATCAGGGAGATCTCGCTGCTCAAGGAACTGAAGCACCCCAACATCGTCCGACTGCTGGACGTGGTGCACAACGAGAGGAAGCTCTATCTGGTGTTTGAGTTCCTCAGCCAGGACCTGAAGAAGTACATGGACTCCACCCCAGGCTCAGAGCTCCCCCTGCACCTCATCAAGAGCTACCTCTTCCAGCTGCTGCAGGGGGTGAGTTTCTGCCACTCACATCGGGTCATCCACCGAGACCTGAAGCCCCAGAACCTGCTCATCAATGAGTTGGGTGCCATCAAGCTGGCTGACTTCGGCCTGGCTCGCGCCTTCGGGGTGCCCCTGCGCACCTACACCCATGAGGTGGTGACACTGTGGTATCGCGCCCCCGAGATTCTCTTGGGCAGCAAGTTCTATACCACAGCTGTGGATATCTGGAGCATTGGTTGCATCTTTGCAGAGATGGTGACTCGAAAAGCCCTGTTTCCTGGTGACTCTGAGATTGACCAGCTCTTTCGTATCTTTCGTATGCTGGGGACACCCAGCGAAGACACATGGCCCGGGGTCACCCAGCTGCCTGACTATAAGGGCAGCTTCCCTAAGTGGACCAGGAAGGGACTGGAAGAGATTGTGCCCAATCTGGAGCCAGAGGGCAGGGACCTGCTCATGCAACTCCTGCAGTATGACCCCAGCCAGCGGATCACAGCCAAGACTGCCCTGGCCCACCCGTACTTCTCATCCCCTGAGCCCTCCCCAGCTGCCCGCCAGTATGTGCTGCAGCGATTCCGCCATTGAGAATGTCAAGGCCACACTCAGATCCTTTCTCGAGCAGCAGCTGCTGCCCCAGCTGCCTCCTACCCATTGCCAAGAGAGGATGCATCTGGGGAGAGCAAAGCACTAAGGAATTCAGCATCAGCCTGCAGAGGGCTGAGTCTGGGTTAGTCCTGCCC PRIMER_LEFT_EXPLAIN=considered 9505, would not amplify any of the ORF 6576, GC content failed 22, low tm 213, high tm 1911, high 3' stability 39, ok 744 PRIMER_RIGHT_EXPLAIN=considered 9326, GC content failed 54, low tm 682, high tm 6041, high any compl 1, high end compl 3, high 3' stability 58, ok 2487 PRIMER_PAIR_EXPLAIN=considered 22, ok 22 PRIMER_STOP_CODON_POSITION=1003 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=9.663260 PRIMER_LEFT_0_PENALTY=5.563683 PRIMER_RIGHT_0_PENALTY=4.099578 PRIMER_LEFT_0_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_0_SEQUENCE=ACATTCTCAATGGCGGAATC PRIMER_LEFT_0=88,25 PRIMER_RIGHT_0=1011,20 PRIMER_LEFT_0_TM=59.436 PRIMER_RIGHT_0_TM=59.900 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=4.000000 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=924 PRIMER_PAIR_1_PENALTY=10.331136 PRIMER_LEFT_1_PENALTY=6.231558 PRIMER_RIGHT_1_PENALTY=4.099578 PRIMER_LEFT_1_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_1_SEQUENCE=ACATTCTCAATGGCGGAATC PRIMER_LEFT_1=88,24 PRIMER_RIGHT_1=1011,20 PRIMER_LEFT_1_TM=57.768 PRIMER_RIGHT_1_TM=59.900 PRIMER_LEFT_1_GC_PERCENT=41.667 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=4.000000 PRIMER_LEFT_1_END_STABILITY=5.7000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=924 PRIMER_PAIR_2_PENALTY=10.521269 PRIMER_LEFT_2_PENALTY=5.563683 PRIMER_RIGHT_2_PENALTY=4.957587 PRIMER_LEFT_2_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_2_SEQUENCE=ATTCTCAATGGCGGAATCG PRIMER_LEFT_2=88,25 PRIMER_RIGHT_2=1009,19 PRIMER_LEFT_2_TM=59.436 PRIMER_RIGHT_2_TM=60.958 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=47.368 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=3.000000 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=8.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=922 PRIMER_PAIR_3_PENALTY=10.670942 PRIMER_LEFT_3_PENALTY=6.571364 PRIMER_RIGHT_3_PENALTY=4.099578 PRIMER_LEFT_3_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA PRIMER_RIGHT_3_SEQUENCE=ACATTCTCAATGGCGGAATC PRIMER_LEFT_3=88,26 PRIMER_RIGHT_3=1011,20 PRIMER_LEFT_3_TM=60.571 PRIMER_RIGHT_3_TM=59.900 PRIMER_LEFT_3_GC_PERCENT=38.462 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=4.000000 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=924 PRIMER_PAIR_4_PENALTY=11.048511 PRIMER_LEFT_4_PENALTY=5.563683 PRIMER_RIGHT_4_PENALTY=5.484828 PRIMER_LEFT_4_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_4_SEQUENCE=CATTCTCAATGGCGGAATC PRIMER_LEFT_4=88,25 PRIMER_RIGHT_4=1010,19 PRIMER_LEFT_4_TM=59.436 PRIMER_RIGHT_4_TM=59.015 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=47.368 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=3.500000 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=923 PRIMER_PAIR_5_PENALTY=11.189145 PRIMER_LEFT_5_PENALTY=6.231558 PRIMER_RIGHT_5_PENALTY=4.957587 PRIMER_LEFT_5_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_5_SEQUENCE=ATTCTCAATGGCGGAATCG PRIMER_LEFT_5=88,24 PRIMER_RIGHT_5=1009,19 PRIMER_LEFT_5_TM=57.768 PRIMER_RIGHT_5_TM=60.958 PRIMER_LEFT_5_GC_PERCENT=41.667 PRIMER_RIGHT_5_GC_PERCENT=47.368 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=2.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=3.000000 PRIMER_LEFT_5_END_STABILITY=5.7000 PRIMER_RIGHT_5_END_STABILITY=8.6000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PAIR_5_PRODUCT_SIZE=922 PRIMER_PAIR_6_PENALTY=11.528951 PRIMER_LEFT_6_PENALTY=6.571364 PRIMER_RIGHT_6_PENALTY=4.957587 PRIMER_LEFT_6_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA PRIMER_RIGHT_6_SEQUENCE=ATTCTCAATGGCGGAATCG PRIMER_LEFT_6=88,26 PRIMER_RIGHT_6=1009,19 PRIMER_LEFT_6_TM=60.571 PRIMER_RIGHT_6_TM=60.958 PRIMER_LEFT_6_GC_PERCENT=38.462 PRIMER_RIGHT_6_GC_PERCENT=47.368 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=4.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=3.000000 PRIMER_LEFT_6_END_STABILITY=6.7000 PRIMER_RIGHT_6_END_STABILITY=8.6000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PAIR_6_PRODUCT_SIZE=922 PRIMER_PAIR_7_PENALTY=11.716386 PRIMER_LEFT_7_PENALTY=6.231558 PRIMER_RIGHT_7_PENALTY=5.484828 PRIMER_LEFT_7_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_7_SEQUENCE=CATTCTCAATGGCGGAATC PRIMER_LEFT_7=88,24 PRIMER_RIGHT_7=1010,19 PRIMER_LEFT_7_TM=57.768 PRIMER_RIGHT_7_TM=59.015 PRIMER_LEFT_7_GC_PERCENT=41.667 PRIMER_RIGHT_7_GC_PERCENT=47.368 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=5.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=3.500000 PRIMER_LEFT_7_END_STABILITY=5.7000 PRIMER_RIGHT_7_END_STABILITY=6.6000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PAIR_7_PRODUCT_SIZE=923 PRIMER_PAIR_8_PENALTY=12.056192 PRIMER_LEFT_8_PENALTY=6.571364 PRIMER_RIGHT_8_PENALTY=5.484828 PRIMER_LEFT_8_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA PRIMER_RIGHT_8_SEQUENCE=CATTCTCAATGGCGGAATC PRIMER_LEFT_8=88,26 PRIMER_RIGHT_8=1010,19 PRIMER_LEFT_8_TM=60.571 PRIMER_RIGHT_8_TM=59.015 PRIMER_LEFT_8_GC_PERCENT=38.462 PRIMER_RIGHT_8_GC_PERCENT=47.368 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=3.500000 PRIMER_LEFT_8_END_STABILITY=6.7000 PRIMER_RIGHT_8_END_STABILITY=6.6000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PAIR_8_PRODUCT_SIZE=923 PRIMER_PAIR_9_PENALTY=12.416538 PRIMER_LEFT_9_PENALTY=8.316960 PRIMER_RIGHT_9_PENALTY=4.099578 PRIMER_LEFT_9_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAAG PRIMER_RIGHT_9_SEQUENCE=ACATTCTCAATGGCGGAATC PRIMER_LEFT_9=88,27 PRIMER_RIGHT_9=1011,20 PRIMER_LEFT_9_TM=61.317 PRIMER_RIGHT_9_TM=59.900 PRIMER_LEFT_9_GC_PERCENT=40.741 PRIMER_RIGHT_9_GC_PERCENT=45.000 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=5.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=4.000000 PRIMER_LEFT_9_END_STABILITY=6.7000 PRIMER_RIGHT_9_END_STABILITY=6.6000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=924 PRIMER_PAIR_10_PENALTY=12.669516 PRIMER_LEFT_10_PENALTY=5.563683 PRIMER_RIGHT_10_PENALTY=7.105834 PRIMER_LEFT_10_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_10_SEQUENCE=TCTCAATGGCGGAATCGCT PRIMER_LEFT_10=88,25 PRIMER_RIGHT_10=1007,19 PRIMER_LEFT_10_TM=59.436 PRIMER_RIGHT_10_TM=64.106 PRIMER_LEFT_10_GC_PERCENT=40.000 PRIMER_RIGHT_10_GC_PERCENT=52.632 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=3.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=2.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=2.000000 PRIMER_LEFT_10_END_STABILITY=6.4000 PRIMER_RIGHT_10_END_STABILITY=9.9000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=3.00 PRIMER_PAIR_10_PRODUCT_SIZE=920 PRIMER_PAIR_11_PENALTY=12.776612 PRIMER_LEFT_11_PENALTY=5.563683 PRIMER_RIGHT_11_PENALTY=7.212929 PRIMER_LEFT_11_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_11_SEQUENCE=CATTCTCAATGGCGGAATCG PRIMER_LEFT_11=88,25 PRIMER_RIGHT_11=1010,20 PRIMER_LEFT_11_TM=59.436 PRIMER_RIGHT_11_TM=63.713 PRIMER_LEFT_11_GC_PERCENT=40.000 PRIMER_RIGHT_11_GC_PERCENT=50.000 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=5.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=3.500000 PRIMER_LEFT_11_END_STABILITY=6.4000 PRIMER_RIGHT_11_END_STABILITY=8.6000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=1.00 PRIMER_PAIR_11_PRODUCT_SIZE=923 PRIMER_PAIR_12_PENALTY=12.799060 PRIMER_LEFT_12_PENALTY=5.563683 PRIMER_RIGHT_12_PENALTY=7.235377 PRIMER_LEFT_12_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_12_SEQUENCE=GACATTCTCAATGGCGGAATC PRIMER_LEFT_12=88,25 PRIMER_RIGHT_12=1012,21 PRIMER_LEFT_12_TM=59.436 PRIMER_RIGHT_12_TM=61.735 PRIMER_LEFT_12_GC_PERCENT=40.000 PRIMER_RIGHT_12_GC_PERCENT=47.619 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=5.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=3.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=4.500000 PRIMER_LEFT_12_END_STABILITY=6.4000 PRIMER_RIGHT_12_END_STABILITY=6.6000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PAIR_12_PRODUCT_SIZE=925 PRIMER_PAIR_13_PENALTY=13.055628 PRIMER_LEFT_13_PENALTY=5.563683 PRIMER_RIGHT_13_PENALTY=7.491945 PRIMER_LEFT_13_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_13_SEQUENCE=ATTCTCAATGGCGGAATCGC PRIMER_LEFT_13=88,25 PRIMER_RIGHT_13=1009,20 PRIMER_LEFT_13_TM=59.436 PRIMER_RIGHT_13_TM=64.492 PRIMER_LEFT_13_GC_PERCENT=40.000 PRIMER_RIGHT_13_GC_PERCENT=50.000 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=3.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=3.000000 PRIMER_LEFT_13_END_STABILITY=6.4000 PRIMER_RIGHT_13_END_STABILITY=9.8000 PRIMER_PAIR_13_COMPL_ANY=4.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PAIR_13_PRODUCT_SIZE=922 PRIMER_PAIR_14_PENALTY=13.274547 PRIMER_LEFT_14_PENALTY=8.316960 PRIMER_RIGHT_14_PENALTY=4.957587 PRIMER_LEFT_14_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAAG PRIMER_RIGHT_14_SEQUENCE=ATTCTCAATGGCGGAATCG PRIMER_LEFT_14=88,27 PRIMER_RIGHT_14=1009,19 PRIMER_LEFT_14_TM=61.317 PRIMER_RIGHT_14_TM=60.958 PRIMER_LEFT_14_GC_PERCENT=40.741 PRIMER_RIGHT_14_GC_PERCENT=47.368 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=4.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=3.000000 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=8.6000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=0.00 PRIMER_PAIR_14_PRODUCT_SIZE=922 PRIMER_PAIR_15_PENALTY=13.337392 PRIMER_LEFT_15_PENALTY=6.231558 PRIMER_RIGHT_15_PENALTY=7.105834 PRIMER_LEFT_15_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_15_SEQUENCE=TCTCAATGGCGGAATCGCT PRIMER_LEFT_15=88,24 PRIMER_RIGHT_15=1007,19 PRIMER_LEFT_15_TM=57.768 PRIMER_RIGHT_15_TM=64.106 PRIMER_LEFT_15_GC_PERCENT=41.667 PRIMER_RIGHT_15_GC_PERCENT=52.632 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=3.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=2.000000 PRIMER_LEFT_15_END_STABILITY=5.7000 PRIMER_RIGHT_15_END_STABILITY=9.9000 PRIMER_PAIR_15_COMPL_ANY=4.00 PRIMER_PAIR_15_COMPL_END=2.00 PRIMER_PAIR_15_PRODUCT_SIZE=920 PRIMER_PAIR_16_PENALTY=13.444487 PRIMER_LEFT_16_PENALTY=6.231558 PRIMER_RIGHT_16_PENALTY=7.212929 PRIMER_LEFT_16_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_16_SEQUENCE=CATTCTCAATGGCGGAATCG PRIMER_LEFT_16=88,24 PRIMER_RIGHT_16=1010,20 PRIMER_LEFT_16_TM=57.768 PRIMER_RIGHT_16_TM=63.713 PRIMER_LEFT_16_GC_PERCENT=41.667 PRIMER_RIGHT_16_GC_PERCENT=50.000 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=5.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=2.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=3.500000 PRIMER_LEFT_16_END_STABILITY=5.7000 PRIMER_RIGHT_16_END_STABILITY=8.6000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=0.00 PRIMER_PAIR_16_PRODUCT_SIZE=923 PRIMER_PAIR_17_PENALTY=13.466935 PRIMER_LEFT_17_PENALTY=6.231558 PRIMER_RIGHT_17_PENALTY=7.235377 PRIMER_LEFT_17_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_17_SEQUENCE=GACATTCTCAATGGCGGAATC PRIMER_LEFT_17=88,24 PRIMER_RIGHT_17=1012,21 PRIMER_LEFT_17_TM=57.768 PRIMER_RIGHT_17_TM=61.735 PRIMER_LEFT_17_GC_PERCENT=41.667 PRIMER_RIGHT_17_GC_PERCENT=47.619 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=5.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=3.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=4.500000 PRIMER_LEFT_17_END_STABILITY=5.7000 PRIMER_RIGHT_17_END_STABILITY=6.6000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PAIR_17_PRODUCT_SIZE=925 PRIMER_PAIR_18_PENALTY=13.522601 PRIMER_LEFT_18_PENALTY=5.563683 PRIMER_RIGHT_18_PENALTY=7.958919 PRIMER_LEFT_18_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_18_SEQUENCE=TTCTCAATGGCGGAATCGC PRIMER_LEFT_18=88,25 PRIMER_RIGHT_18=1008,19 PRIMER_LEFT_18_TM=59.436 PRIMER_RIGHT_18_TM=64.459 PRIMER_LEFT_18_GC_PERCENT=40.000 PRIMER_RIGHT_18_GC_PERCENT=52.632 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=3.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=3.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=2.500000 PRIMER_LEFT_18_END_STABILITY=6.4000 PRIMER_RIGHT_18_END_STABILITY=9.8000 PRIMER_PAIR_18_COMPL_ANY=4.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PAIR_18_PRODUCT_SIZE=921 PRIMER_PAIR_19_PENALTY=13.677198 PRIMER_LEFT_19_PENALTY=6.571364 PRIMER_RIGHT_19_PENALTY=7.105834 PRIMER_LEFT_19_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA PRIMER_RIGHT_19_SEQUENCE=TCTCAATGGCGGAATCGCT PRIMER_LEFT_19=88,26 PRIMER_RIGHT_19=1007,19 PRIMER_LEFT_19_TM=60.571 PRIMER_RIGHT_19_TM=64.106 PRIMER_LEFT_19_GC_PERCENT=38.462 PRIMER_RIGHT_19_GC_PERCENT=52.632 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=3.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=2.000000 PRIMER_LEFT_19_END_STABILITY=6.7000 PRIMER_RIGHT_19_END_STABILITY=9.9000 PRIMER_PAIR_19_COMPL_ANY=4.00 PRIMER_PAIR_19_COMPL_END=2.00 PRIMER_PAIR_19_PRODUCT_SIZE=920 = SEQUENCE_ID=X66364/CDK5 P3_COMMENT=H.sapiens mRNA PSSALRE for serine/threonine protein kinase. CDS_25..903 SEQUENCE_START_CODON_POSITION=24 SEQUENCE_TEMPLATE=CGCAGGGGTCCCCCGGCCGCCGCGATGCAGAAATACGAGAAACTGGAAAAGATTGGGGAAGGCACCTACGGAACTGTGTTCAAGGCCAAAAACCGGGAGACTCATGAGATCGTGGCTCTGAAACGGGTGAGGCTGGATGACGATGATGAGGGTGTGCCGAGTTCCGCCCTCCGGGAGATCTGCCTACTCAAGGAGCTGAAGCACAAGAACATCGTCAGGCTTCATGACGTCCTGCACAGCGACAAGAAGCTGACTTTGGTTTTTGAATTCTGTGACCAGGACCTGAAGAAGTATTTTGACAGTTGCAATGGTGACCTCGATCCTGAGATTGTAAAGTCATTCCTCTTCCAGCTACTAAAAGGGCTGGGATTCTGTCATAGCCGCAATGTGCTACACAGGGACCTGAAGCCCCAGAACCTGCTAATAAACAGGAATGGGGAGCTGAAATTGGCTGATTTTGGCCTGGCTCGAGCCTTTGGGATTCCCGTCCGCTGTTACTCAGCTGAGGTGGTCACACTGTGGTACCGCCCACCGGATGTCCTCTTTGGGGCCAAGCTGTACTCCACGTCCATCGACATGTGGTCAGCCGGCTGCATCTTTGCAGAGCTGGCCAATGCTGGGCGGCCTCTTTTTCCCGGCAATGATGTCGATGACCAGTTGAAGAGGATCTTCCGACTGCTGGGGACGCCCACCGAGGAGCAGTGGCCCTCTATGACCAAGCTGCCAGACTATAAGCCCTATCCGATGTACCCGGCCACAACATCCCTGGTGAACGTCGTGCCCAAACTCAATGCCACAGGGAGGGATCTGCTGCAGAACCTTCTGAAGTGTAACCCTGTCCAGCGTATCTCAGCAGAAGAGGCCCTGCAGCACCCCTACTTCTCCGACTTCTGTCCGCCCTAGGCCCCGGGACCCCCGCCTCCAGGCTGGGCCTGGCCTATTTAAGCCCCCTCTTGAGAGGGTGAGACAGTGGGGGTGCCTGG PRIMER_LEFT_EXPLAIN=considered 7904, would not amplify any of the ORF 5278, GC content failed 48, low tm 249, high tm 1503, high 3' stability 40, ok 786 PRIMER_RIGHT_EXPLAIN=considered 7709, GC content failed 194, low tm 667, high tm 4515, high 3' stability 60, ok 2273 PRIMER_PAIR_EXPLAIN=considered 21, ok 21 PRIMER_STOP_CODON_POSITION=900 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=5.830871 PRIMER_LEFT_0_PENALTY=3.142611 PRIMER_RIGHT_0_PENALTY=2.688260 PRIMER_LEFT_0_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_0_SEQUENCE=CCTAGGGCGGACAGAAGTC PRIMER_LEFT_0=24,23 PRIMER_RIGHT_0=903,19 PRIMER_LEFT_0_TM=60.143 PRIMER_RIGHT_0_TM=59.812 PRIMER_LEFT_0_GC_PERCENT=39.130 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=1.500000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=880 PRIMER_PAIR_1_PENALTY=6.081075 PRIMER_LEFT_1_PENALTY=3.142611 PRIMER_RIGHT_1_PENALTY=2.938464 PRIMER_LEFT_1_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_1_SEQUENCE=CTAGGGCGGACAGAAGTCG PRIMER_LEFT_1=24,23 PRIMER_RIGHT_1=902,19 PRIMER_LEFT_1_TM=60.143 PRIMER_RIGHT_1_TM=60.938 PRIMER_LEFT_1_GC_PERCENT=39.130 PRIMER_RIGHT_1_GC_PERCENT=63.158 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=1.000000 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=879 PRIMER_PAIR_2_PENALTY=6.285566 PRIMER_LEFT_2_PENALTY=3.597306 PRIMER_RIGHT_2_PENALTY=2.688260 PRIMER_LEFT_2_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_2_SEQUENCE=CCTAGGGCGGACAGAAGTC PRIMER_LEFT_2=24,22 PRIMER_RIGHT_2=903,19 PRIMER_LEFT_2_TM=58.403 PRIMER_RIGHT_2_TM=59.812 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=63.158 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=1.500000 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=880 PRIMER_PAIR_3_PENALTY=6.535770 PRIMER_LEFT_3_PENALTY=3.597306 PRIMER_RIGHT_3_PENALTY=2.938464 PRIMER_LEFT_3_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_3_SEQUENCE=CTAGGGCGGACAGAAGTCG PRIMER_LEFT_3=24,22 PRIMER_RIGHT_3=902,19 PRIMER_LEFT_3_TM=58.403 PRIMER_RIGHT_3_TM=60.938 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=63.158 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=1.000000 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=879 PRIMER_PAIR_4_PENALTY=7.847014 PRIMER_LEFT_4_PENALTY=3.142611 PRIMER_RIGHT_4_PENALTY=4.704404 PRIMER_LEFT_4_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_4_SEQUENCE=GCCTAGGGCGGACAGAAGT PRIMER_LEFT_4=24,23 PRIMER_RIGHT_4=904,19 PRIMER_LEFT_4_TM=60.143 PRIMER_RIGHT_4_TM=61.704 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=63.158 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=2.000000 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=881 PRIMER_PAIR_5_PENALTY=7.990463 PRIMER_LEFT_5_PENALTY=5.302203 PRIMER_RIGHT_5_PENALTY=2.688260 PRIMER_LEFT_5_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA PRIMER_RIGHT_5_SEQUENCE=CCTAGGGCGGACAGAAGTC PRIMER_LEFT_5=24,24 PRIMER_RIGHT_5=903,19 PRIMER_LEFT_5_TM=61.302 PRIMER_RIGHT_5_TM=59.812 PRIMER_LEFT_5_GC_PERCENT=37.500 PRIMER_RIGHT_5_GC_PERCENT=63.158 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=2.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=1.500000 PRIMER_LEFT_5_END_STABILITY=8.5000 PRIMER_RIGHT_5_END_STABILITY=6.4000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=880 PRIMER_PAIR_6_PENALTY=8.240667 PRIMER_LEFT_6_PENALTY=5.302203 PRIMER_RIGHT_6_PENALTY=2.938464 PRIMER_LEFT_6_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA PRIMER_RIGHT_6_SEQUENCE=CTAGGGCGGACAGAAGTCG PRIMER_LEFT_6=24,24 PRIMER_RIGHT_6=902,19 PRIMER_LEFT_6_TM=61.302 PRIMER_RIGHT_6_TM=60.938 PRIMER_LEFT_6_GC_PERCENT=37.500 PRIMER_RIGHT_6_GC_PERCENT=63.158 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=4.00 PRIMER_LEFT_6_SELF_END=1.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=1.000000 PRIMER_LEFT_6_END_STABILITY=8.5000 PRIMER_RIGHT_6_END_STABILITY=8.1000 PRIMER_PAIR_6_COMPL_ANY=3.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PAIR_6_PRODUCT_SIZE=879 PRIMER_PAIR_7_PENALTY=8.244153 PRIMER_LEFT_7_PENALTY=3.142611 PRIMER_RIGHT_7_PENALTY=5.101542 PRIMER_LEFT_7_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_7_SEQUENCE=TAGGGCGGACAGAAGTCGG PRIMER_LEFT_7=24,23 PRIMER_RIGHT_7=901,19 PRIMER_LEFT_7_TM=60.143 PRIMER_RIGHT_7_TM=63.602 PRIMER_LEFT_7_GC_PERCENT=39.130 PRIMER_RIGHT_7_GC_PERCENT=63.158 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=3.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=0.500000 PRIMER_LEFT_7_END_STABILITY=8.2000 PRIMER_RIGHT_7_END_STABILITY=9.6000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PAIR_7_PRODUCT_SIZE=878 PRIMER_PAIR_8_PENALTY=8.301709 PRIMER_LEFT_8_PENALTY=3.597306 PRIMER_RIGHT_8_PENALTY=4.704404 PRIMER_LEFT_8_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_8_SEQUENCE=GCCTAGGGCGGACAGAAGT PRIMER_LEFT_8=24,22 PRIMER_RIGHT_8=904,19 PRIMER_LEFT_8_TM=58.403 PRIMER_RIGHT_8_TM=61.704 PRIMER_LEFT_8_GC_PERCENT=40.909 PRIMER_RIGHT_8_GC_PERCENT=63.158 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=1.00 PRIMER_RIGHT_8_SELF_END=1.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=2.000000 PRIMER_LEFT_8_END_STABILITY=7.9000 PRIMER_RIGHT_8_END_STABILITY=6.4000 PRIMER_PAIR_8_COMPL_ANY=3.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PAIR_8_PRODUCT_SIZE=881 PRIMER_PAIR_9_PENALTY=8.517669 PRIMER_LEFT_9_PENALTY=3.142611 PRIMER_RIGHT_9_PENALTY=5.375058 PRIMER_LEFT_9_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_9_SEQUENCE=CTAGGGCGGACAGAAGTCGG PRIMER_LEFT_9=24,23 PRIMER_RIGHT_9=902,20 PRIMER_LEFT_9_TM=60.143 PRIMER_RIGHT_9_TM=64.375 PRIMER_LEFT_9_GC_PERCENT=39.130 PRIMER_RIGHT_9_GC_PERCENT=65.000 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=1.000000 PRIMER_LEFT_9_END_STABILITY=8.2000 PRIMER_RIGHT_9_END_STABILITY=9.6000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PAIR_9_PRODUCT_SIZE=879 PRIMER_PAIR_10_PENALTY=8.611012 PRIMER_LEFT_10_PENALTY=3.142611 PRIMER_RIGHT_10_PENALTY=5.468402 PRIMER_LEFT_10_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_10_SEQUENCE=GCCTAGGGCGGACAGAAGTC PRIMER_LEFT_10=24,23 PRIMER_RIGHT_10=904,20 PRIMER_LEFT_10_TM=60.143 PRIMER_RIGHT_10_TM=63.468 PRIMER_LEFT_10_GC_PERCENT=39.130 PRIMER_RIGHT_10_GC_PERCENT=65.000 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=2.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=2.000000 PRIMER_LEFT_10_END_STABILITY=8.2000 PRIMER_RIGHT_10_END_STABILITY=6.4000 PRIMER_PAIR_10_COMPL_ANY=3.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PAIR_10_PRODUCT_SIZE=881 PRIMER_PAIR_11_PENALTY=8.698847 PRIMER_LEFT_11_PENALTY=3.597306 PRIMER_RIGHT_11_PENALTY=5.101542 PRIMER_LEFT_11_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_11_SEQUENCE=TAGGGCGGACAGAAGTCGG PRIMER_LEFT_11=24,22 PRIMER_RIGHT_11=901,19 PRIMER_LEFT_11_TM=58.403 PRIMER_RIGHT_11_TM=63.602 PRIMER_LEFT_11_GC_PERCENT=40.909 PRIMER_RIGHT_11_GC_PERCENT=63.158 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=3.00 PRIMER_LEFT_11_SELF_END=1.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=0.500000 PRIMER_LEFT_11_END_STABILITY=7.9000 PRIMER_RIGHT_11_END_STABILITY=9.6000 PRIMER_PAIR_11_COMPL_ANY=3.00 PRIMER_PAIR_11_COMPL_END=0.00 PRIMER_PAIR_11_PRODUCT_SIZE=878 PRIMER_PAIR_12_PENALTY=8.972363 PRIMER_LEFT_12_PENALTY=3.597306 PRIMER_RIGHT_12_PENALTY=5.375058 PRIMER_LEFT_12_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_12_SEQUENCE=CTAGGGCGGACAGAAGTCGG PRIMER_LEFT_12=24,22 PRIMER_RIGHT_12=902,20 PRIMER_LEFT_12_TM=58.403 PRIMER_RIGHT_12_TM=64.375 PRIMER_LEFT_12_GC_PERCENT=40.909 PRIMER_RIGHT_12_GC_PERCENT=65.000 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=1.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=1.000000 PRIMER_LEFT_12_END_STABILITY=7.9000 PRIMER_RIGHT_12_END_STABILITY=9.6000 PRIMER_PAIR_12_COMPL_ANY=3.00 PRIMER_PAIR_12_COMPL_END=0.00 PRIMER_PAIR_12_PRODUCT_SIZE=879 PRIMER_PAIR_13_PENALTY=9.017669 PRIMER_LEFT_13_PENALTY=3.142611 PRIMER_RIGHT_13_PENALTY=5.875058 PRIMER_LEFT_13_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_13_SEQUENCE=CCTAGGGCGGACAGAAGTCG PRIMER_LEFT_13=24,23 PRIMER_RIGHT_13=903,20 PRIMER_LEFT_13_TM=60.143 PRIMER_RIGHT_13_TM=64.375 PRIMER_LEFT_13_GC_PERCENT=39.130 PRIMER_RIGHT_13_GC_PERCENT=65.000 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=6.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=1.500000 PRIMER_LEFT_13_END_STABILITY=8.2000 PRIMER_RIGHT_13_END_STABILITY=8.1000 PRIMER_PAIR_13_COMPL_ANY=3.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PAIR_13_PRODUCT_SIZE=880 PRIMER_PAIR_14_PENALTY=9.065707 PRIMER_LEFT_14_PENALTY=3.597306 PRIMER_RIGHT_14_PENALTY=5.468402 PRIMER_LEFT_14_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_14_SEQUENCE=GCCTAGGGCGGACAGAAGTC PRIMER_LEFT_14=24,22 PRIMER_RIGHT_14=904,20 PRIMER_LEFT_14_TM=58.403 PRIMER_RIGHT_14_TM=63.468 PRIMER_LEFT_14_GC_PERCENT=40.909 PRIMER_RIGHT_14_GC_PERCENT=65.000 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=1.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=2.000000 PRIMER_LEFT_14_END_STABILITY=7.9000 PRIMER_RIGHT_14_END_STABILITY=6.4000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=1.00 PRIMER_PAIR_14_PRODUCT_SIZE=881 PRIMER_PAIR_15_PENALTY=9.472363 PRIMER_LEFT_15_PENALTY=3.597306 PRIMER_RIGHT_15_PENALTY=5.875058 PRIMER_LEFT_15_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_15_SEQUENCE=CCTAGGGCGGACAGAAGTCG PRIMER_LEFT_15=24,22 PRIMER_RIGHT_15=903,20 PRIMER_LEFT_15_TM=58.403 PRIMER_RIGHT_15_TM=64.375 PRIMER_LEFT_15_GC_PERCENT=40.909 PRIMER_RIGHT_15_GC_PERCENT=65.000 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=1.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=1.500000 PRIMER_LEFT_15_END_STABILITY=7.9000 PRIMER_RIGHT_15_END_STABILITY=8.1000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=880 PRIMER_PAIR_16_PENALTY=10.006607 PRIMER_LEFT_16_PENALTY=5.302203 PRIMER_RIGHT_16_PENALTY=4.704404 PRIMER_LEFT_16_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA PRIMER_RIGHT_16_SEQUENCE=GCCTAGGGCGGACAGAAGT PRIMER_LEFT_16=24,24 PRIMER_RIGHT_16=904,19 PRIMER_LEFT_16_TM=61.302 PRIMER_RIGHT_16_TM=61.704 PRIMER_LEFT_16_GC_PERCENT=37.500 PRIMER_RIGHT_16_GC_PERCENT=63.158 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=1.00 PRIMER_RIGHT_16_SELF_END=1.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=2.000000 PRIMER_LEFT_16_END_STABILITY=8.5000 PRIMER_RIGHT_16_END_STABILITY=6.4000 PRIMER_PAIR_16_COMPL_ANY=3.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PAIR_16_PRODUCT_SIZE=881 PRIMER_PAIR_17_PENALTY=10.044329 PRIMER_LEFT_17_PENALTY=7.356069 PRIMER_RIGHT_17_PENALTY=2.688260 PRIMER_LEFT_17_SEQUENCE=ATGCAGAAATACGAGAAACTGGAAA PRIMER_RIGHT_17_SEQUENCE=CCTAGGGCGGACAGAAGTC PRIMER_LEFT_17=24,25 PRIMER_RIGHT_17=903,19 PRIMER_LEFT_17_TM=62.356 PRIMER_RIGHT_17_TM=59.812 PRIMER_LEFT_17_GC_PERCENT=36.000 PRIMER_RIGHT_17_GC_PERCENT=63.158 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=6.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=1.500000 PRIMER_LEFT_17_END_STABILITY=8.5000 PRIMER_RIGHT_17_END_STABILITY=6.4000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=0.00 PRIMER_PAIR_17_PRODUCT_SIZE=880 PRIMER_PAIR_18_PENALTY=10.294533 PRIMER_LEFT_18_PENALTY=7.356069 PRIMER_RIGHT_18_PENALTY=2.938464 PRIMER_LEFT_18_SEQUENCE=ATGCAGAAATACGAGAAACTGGAAA PRIMER_RIGHT_18_SEQUENCE=CTAGGGCGGACAGAAGTCG PRIMER_LEFT_18=24,25 PRIMER_RIGHT_18=902,19 PRIMER_LEFT_18_TM=62.356 PRIMER_RIGHT_18_TM=60.938 PRIMER_LEFT_18_GC_PERCENT=36.000 PRIMER_RIGHT_18_GC_PERCENT=63.158 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=1.000000 PRIMER_LEFT_18_END_STABILITY=8.5000 PRIMER_RIGHT_18_END_STABILITY=8.1000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PAIR_18_PRODUCT_SIZE=879 PRIMER_PAIR_19_PENALTY=10.403745 PRIMER_LEFT_19_PENALTY=5.302203 PRIMER_RIGHT_19_PENALTY=5.101542 PRIMER_LEFT_19_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA PRIMER_RIGHT_19_SEQUENCE=TAGGGCGGACAGAAGTCGG PRIMER_LEFT_19=24,24 PRIMER_RIGHT_19=901,19 PRIMER_LEFT_19_TM=61.302 PRIMER_RIGHT_19_TM=63.602 PRIMER_LEFT_19_GC_PERCENT=37.500 PRIMER_RIGHT_19_GC_PERCENT=63.158 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=3.00 PRIMER_LEFT_19_SELF_END=1.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=0.500000 PRIMER_LEFT_19_END_STABILITY=8.5000 PRIMER_RIGHT_19_END_STABILITY=9.6000 PRIMER_PAIR_19_COMPL_ANY=3.00 PRIMER_PAIR_19_COMPL_END=0.00 PRIMER_PAIR_19_PRODUCT_SIZE=878 = SEQUENCE_ID=L34673/HIP116 P3_COMMENT=Human ATPase, DNA-binding protein (HIP116) mRNA, 3' end. CDS_178..3207 SEQUENCE_START_CODON_POSITION=177 SEQUENCE_TEMPLATE=ATTCCCGGGGTCTGACTGGACTCGCGGCGACTTACCTTTCAGTCGTGCGCTCCTGATCCGGCGCTCGGAATTTGTCCCCGGCTTCAGGGCTGCGGGGCCTGGAAGGAGGCGTATCGAGGCGGCTCGAAAACGATCCAGGGGAGCCGAGGCGCTCCTCTTGTCATCCCACTCAGCGCCATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAGTTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCATATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTTGAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAAGTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATTTGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTGGGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAGAAGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGGAATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGTCTACGTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGCAGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGCAGGATCTTCTAAGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTTCCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATAAAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCCAAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCATAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGCGTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGAAAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCTCAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATGAAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCTTTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAGAATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTCTTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGGTGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTCTGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAGCAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAATGAAATCAGAACATTAATTGACTTATAATTTGTGGGATTTTAGTAAGAAGACTACTATATGTGAGAGGCGTGATATCTGGATGGAAGTTGGGCTGGATGATCTCCAAAGTCGTTTCAACTCTTAAAGACATCTTAATCCTGAATGTAAACAATTGTTATGTGTTTAGAATCAGAATTTGATTTTGAACTTGAGTAATTCATCCTTACAGCTATCTGTAGAATTAGTCATCTTTTTTCTT PRIMER_LEFT_EXPLAIN=considered 29448, would not amplify any of the ORF 20059, GC content failed 202, low tm 3593, high tm 1850, long poly-x seq 41, high 3' stability 65, ok 3638 PRIMER_RIGHT_EXPLAIN=considered 29262, GC content failed 608, low tm 11948, high tm 5429, high end compl 9, long poly-x seq 56, high 3' stability 79, ok 11133 PRIMER_PAIR_EXPLAIN=considered 21, ok 21 PRIMER_STOP_CODON_POSITION=3204 PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=20 PRIMER_PAIR_0_PENALTY=15.950720 PRIMER_LEFT_0_PENALTY=1.996678 PRIMER_RIGHT_0_PENALTY=13.954041 PRIMER_LEFT_0_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_0_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT PRIMER_LEFT_0=177,21 PRIMER_RIGHT_0=3214,27 PRIMER_LEFT_0_TM=59.003 PRIMER_RIGHT_0_TM=58.046 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=25.926 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=5.000000 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=3038 PRIMER_PAIR_1_PENALTY=18.477471 PRIMER_LEFT_1_PENALTY=4.523430 PRIMER_RIGHT_1_PENALTY=13.954041 PRIMER_LEFT_1_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG PRIMER_RIGHT_1_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT PRIMER_LEFT_1=177,22 PRIMER_RIGHT_1=3214,27 PRIMER_LEFT_1_TM=62.523 PRIMER_RIGHT_1_TM=58.046 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=25.926 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=5.000000 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=3038 PRIMER_PAIR_2_PENALTY=19.375672 PRIMER_LEFT_2_PENALTY=1.996678 PRIMER_RIGHT_2_PENALTY=17.378993 PRIMER_LEFT_2_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_2_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_2=177,21 PRIMER_RIGHT_2=3221,26 PRIMER_LEFT_2_TM=59.003 PRIMER_RIGHT_2_TM=57.121 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=26.923 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=8.500000 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=3045 PRIMER_PAIR_3_PENALTY=20.047681 PRIMER_LEFT_3_PENALTY=1.996678 PRIMER_RIGHT_3_PENALTY=18.051002 PRIMER_LEFT_3_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_3_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT PRIMER_LEFT_3=177,21 PRIMER_RIGHT_3=3221,27 PRIMER_LEFT_3_TM=59.003 PRIMER_RIGHT_3_TM=57.449 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=25.926 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=8.500000 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=3045 PRIMER_PAIR_4_PENALTY=20.156411 PRIMER_LEFT_4_PENALTY=1.996678 PRIMER_RIGHT_4_PENALTY=18.159733 PRIMER_LEFT_4_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_4_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_4=177,21 PRIMER_RIGHT_4=3222,27 PRIMER_LEFT_4_TM=59.003 PRIMER_RIGHT_4_TM=57.840 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=25.926 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=9.000000 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=3046 PRIMER_PAIR_5_PENALTY=21.147183 PRIMER_LEFT_5_PENALTY=7.193142 PRIMER_RIGHT_5_PENALTY=13.954041 PRIMER_LEFT_5_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA PRIMER_RIGHT_5_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT PRIMER_LEFT_5=177,23 PRIMER_RIGHT_5=3214,27 PRIMER_LEFT_5_TM=64.193 PRIMER_RIGHT_5_TM=58.046 PRIMER_LEFT_5_GC_PERCENT=47.826 PRIMER_RIGHT_5_GC_PERCENT=25.926 PRIMER_LEFT_5_SELF_ANY=5.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=5.000000 PRIMER_LEFT_5_END_STABILITY=9.4000 PRIMER_RIGHT_5_END_STABILITY=6.6000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=3038 PRIMER_PAIR_6_PENALTY=21.902423 PRIMER_LEFT_6_PENALTY=4.523430 PRIMER_RIGHT_6_PENALTY=17.378993 PRIMER_LEFT_6_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG PRIMER_RIGHT_6_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_6=177,22 PRIMER_RIGHT_6=3221,26 PRIMER_LEFT_6_TM=62.523 PRIMER_RIGHT_6_TM=57.121 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=26.923 PRIMER_LEFT_6_SELF_ANY=5.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=8.500000 PRIMER_LEFT_6_END_STABILITY=9.4000 PRIMER_RIGHT_6_END_STABILITY=6.7000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PAIR_6_PRODUCT_SIZE=3045 PRIMER_PAIR_7_PENALTY=22.190785 PRIMER_LEFT_7_PENALTY=8.236744 PRIMER_RIGHT_7_PENALTY=13.954041 PRIMER_LEFT_7_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT PRIMER_RIGHT_7_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT PRIMER_LEFT_7=177,24 PRIMER_RIGHT_7=3214,27 PRIMER_LEFT_7_TM=64.237 PRIMER_RIGHT_7_TM=58.046 PRIMER_LEFT_7_GC_PERCENT=45.833 PRIMER_RIGHT_7_GC_PERCENT=25.926 PRIMER_LEFT_7_SELF_ANY=5.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=5.000000 PRIMER_LEFT_7_END_STABILITY=9.3000 PRIMER_RIGHT_7_END_STABILITY=6.6000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PAIR_7_PRODUCT_SIZE=3038 PRIMER_PAIR_8_PENALTY=22.574432 PRIMER_LEFT_8_PENALTY=4.523430 PRIMER_RIGHT_8_PENALTY=18.051002 PRIMER_LEFT_8_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG PRIMER_RIGHT_8_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT PRIMER_LEFT_8=177,22 PRIMER_RIGHT_8=3221,27 PRIMER_LEFT_8_TM=62.523 PRIMER_RIGHT_8_TM=57.449 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=25.926 PRIMER_LEFT_8_SELF_ANY=5.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=8.500000 PRIMER_LEFT_8_END_STABILITY=9.4000 PRIMER_RIGHT_8_END_STABILITY=6.9000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=0.00 PRIMER_PAIR_8_PRODUCT_SIZE=3045 PRIMER_PAIR_9_PENALTY=22.683163 PRIMER_LEFT_9_PENALTY=4.523430 PRIMER_RIGHT_9_PENALTY=18.159733 PRIMER_LEFT_9_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG PRIMER_RIGHT_9_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_9=177,22 PRIMER_RIGHT_9=3222,27 PRIMER_LEFT_9_TM=62.523 PRIMER_RIGHT_9_TM=57.840 PRIMER_LEFT_9_GC_PERCENT=50.000 PRIMER_RIGHT_9_GC_PERCENT=25.926 PRIMER_LEFT_9_SELF_ANY=5.00 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=0.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=9.000000 PRIMER_LEFT_9_END_STABILITY=9.4000 PRIMER_RIGHT_9_END_STABILITY=6.7000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PAIR_9_PRODUCT_SIZE=3046 PRIMER_PAIR_10_PENALTY=24.254496 PRIMER_LEFT_10_PENALTY=1.996678 PRIMER_RIGHT_10_PENALTY=22.257817 PRIMER_LEFT_10_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_10_SEQUENCE=TCTTCTTACTAAAATCCCACAAATTAT PRIMER_LEFT_10=177,21 PRIMER_RIGHT_10=3229,27 PRIMER_LEFT_10_TM=59.003 PRIMER_RIGHT_10_TM=57.242 PRIMER_LEFT_10_GC_PERCENT=47.619 PRIMER_RIGHT_10_GC_PERCENT=25.926 PRIMER_LEFT_10_SELF_ANY=5.00 PRIMER_RIGHT_10_SELF_ANY=5.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=3.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=12.500000 PRIMER_LEFT_10_END_STABILITY=7.9000 PRIMER_RIGHT_10_END_STABILITY=5.8000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=3.00 PRIMER_PAIR_10_PRODUCT_SIZE=3053 PRIMER_PAIR_11_PENALTY=24.257918 PRIMER_LEFT_11_PENALTY=1.996678 PRIMER_RIGHT_11_PENALTY=22.261240 PRIMER_LEFT_11_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_11_SEQUENCE=AGTCTTCTTACTAAAATCCCACAAAT PRIMER_LEFT_11=177,21 PRIMER_RIGHT_11=3231,26 PRIMER_LEFT_11_TM=59.003 PRIMER_RIGHT_11_TM=57.239 PRIMER_LEFT_11_GC_PERCENT=47.619 PRIMER_RIGHT_11_GC_PERCENT=30.769 PRIMER_LEFT_11_SELF_ANY=5.00 PRIMER_RIGHT_11_SELF_ANY=5.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=13.500000 PRIMER_LEFT_11_END_STABILITY=7.9000 PRIMER_RIGHT_11_END_STABILITY=7.2000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=3.00 PRIMER_PAIR_11_PRODUCT_SIZE=3055 PRIMER_PAIR_12_PENALTY=24.363903 PRIMER_LEFT_12_PENALTY=1.996678 PRIMER_RIGHT_12_PENALTY=22.367225 PRIMER_LEFT_12_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_12_SEQUENCE=GTCTTCTTACTAAAATCCCACAAATTA PRIMER_LEFT_12=177,21 PRIMER_RIGHT_12=3230,27 PRIMER_LEFT_12_TM=59.003 PRIMER_RIGHT_12_TM=57.633 PRIMER_LEFT_12_GC_PERCENT=47.619 PRIMER_RIGHT_12_GC_PERCENT=29.630 PRIMER_LEFT_12_SELF_ANY=5.00 PRIMER_RIGHT_12_SELF_ANY=5.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=13.000000 PRIMER_LEFT_12_END_STABILITY=7.9000 PRIMER_RIGHT_12_END_STABILITY=6.2000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PAIR_12_PRODUCT_SIZE=3054 PRIMER_PAIR_13_PENALTY=24.572135 PRIMER_LEFT_13_PENALTY=7.193142 PRIMER_RIGHT_13_PENALTY=17.378993 PRIMER_LEFT_13_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA PRIMER_RIGHT_13_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_13=177,23 PRIMER_RIGHT_13=3221,26 PRIMER_LEFT_13_TM=64.193 PRIMER_RIGHT_13_TM=57.121 PRIMER_LEFT_13_GC_PERCENT=47.826 PRIMER_RIGHT_13_GC_PERCENT=26.923 PRIMER_LEFT_13_SELF_ANY=5.00 PRIMER_RIGHT_13_SELF_ANY=6.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=0.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=8.500000 PRIMER_LEFT_13_END_STABILITY=9.4000 PRIMER_RIGHT_13_END_STABILITY=6.7000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=0.00 PRIMER_PAIR_13_PRODUCT_SIZE=3045 PRIMER_PAIR_14_PENALTY=25.244145 PRIMER_LEFT_14_PENALTY=7.193142 PRIMER_RIGHT_14_PENALTY=18.051002 PRIMER_LEFT_14_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA PRIMER_RIGHT_14_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT PRIMER_LEFT_14=177,23 PRIMER_RIGHT_14=3221,27 PRIMER_LEFT_14_TM=64.193 PRIMER_RIGHT_14_TM=57.449 PRIMER_LEFT_14_GC_PERCENT=47.826 PRIMER_RIGHT_14_GC_PERCENT=25.926 PRIMER_LEFT_14_SELF_ANY=5.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=8.500000 PRIMER_LEFT_14_END_STABILITY=9.4000 PRIMER_RIGHT_14_END_STABILITY=6.9000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=1.00 PRIMER_PAIR_14_PRODUCT_SIZE=3045 PRIMER_PAIR_15_PENALTY=25.352875 PRIMER_LEFT_15_PENALTY=7.193142 PRIMER_RIGHT_15_PENALTY=18.159733 PRIMER_LEFT_15_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA PRIMER_RIGHT_15_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_15=177,23 PRIMER_RIGHT_15=3222,27 PRIMER_LEFT_15_TM=64.193 PRIMER_RIGHT_15_TM=57.840 PRIMER_LEFT_15_GC_PERCENT=47.826 PRIMER_RIGHT_15_GC_PERCENT=25.926 PRIMER_LEFT_15_SELF_ANY=5.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=9.000000 PRIMER_LEFT_15_END_STABILITY=9.4000 PRIMER_RIGHT_15_END_STABILITY=6.7000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=3046 PRIMER_PAIR_16_PENALTY=25.615737 PRIMER_LEFT_16_PENALTY=8.236744 PRIMER_RIGHT_16_PENALTY=17.378993 PRIMER_LEFT_16_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT PRIMER_RIGHT_16_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_16=177,24 PRIMER_RIGHT_16=3221,26 PRIMER_LEFT_16_TM=64.237 PRIMER_RIGHT_16_TM=57.121 PRIMER_LEFT_16_GC_PERCENT=45.833 PRIMER_RIGHT_16_GC_PERCENT=26.923 PRIMER_LEFT_16_SELF_ANY=5.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=8.500000 PRIMER_LEFT_16_END_STABILITY=9.3000 PRIMER_RIGHT_16_END_STABILITY=6.7000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PAIR_16_PRODUCT_SIZE=3045 PRIMER_PAIR_17_PENALTY=25.667366 PRIMER_LEFT_17_PENALTY=1.996678 PRIMER_RIGHT_17_PENALTY=23.670687 PRIMER_LEFT_17_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_17_SEQUENCE=TAGTCTTCTTACTAAAATCCCACAAAT PRIMER_LEFT_17=177,21 PRIMER_RIGHT_17=3232,27 PRIMER_LEFT_17_TM=59.003 PRIMER_RIGHT_17_TM=57.329 PRIMER_LEFT_17_GC_PERCENT=47.619 PRIMER_RIGHT_17_GC_PERCENT=29.630 PRIMER_LEFT_17_SELF_ANY=5.00 PRIMER_RIGHT_17_SELF_ANY=5.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=14.000000 PRIMER_LEFT_17_END_STABILITY=7.9000 PRIMER_RIGHT_17_END_STABILITY=7.2000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=3.00 PRIMER_PAIR_17_PRODUCT_SIZE=3056 PRIMER_PAIR_18_PENALTY=25.768931 PRIMER_LEFT_18_PENALTY=1.996678 PRIMER_RIGHT_18_PENALTY=23.772253 PRIMER_LEFT_18_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_18_SEQUENCE=GTAGTCTTCTTACTAAAATCCCACAAA PRIMER_LEFT_18=177,21 PRIMER_RIGHT_18=3233,27 PRIMER_LEFT_18_TM=59.003 PRIMER_RIGHT_18_TM=57.728 PRIMER_LEFT_18_GC_PERCENT=47.619 PRIMER_RIGHT_18_GC_PERCENT=33.333 PRIMER_LEFT_18_SELF_ANY=5.00 PRIMER_RIGHT_18_SELF_ANY=5.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=0.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=14.500000 PRIMER_LEFT_18_END_STABILITY=7.9000 PRIMER_RIGHT_18_END_STABILITY=7.0000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PAIR_18_PRODUCT_SIZE=3057 PRIMER_PAIR_19_PENALTY=26.287746 PRIMER_LEFT_19_PENALTY=8.236744 PRIMER_RIGHT_19_PENALTY=18.051002 PRIMER_LEFT_19_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT PRIMER_RIGHT_19_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT PRIMER_LEFT_19=177,24 PRIMER_RIGHT_19=3221,27 PRIMER_LEFT_19_TM=64.237 PRIMER_RIGHT_19_TM=57.449 PRIMER_LEFT_19_GC_PERCENT=45.833 PRIMER_RIGHT_19_GC_PERCENT=25.926 PRIMER_LEFT_19_SELF_ANY=5.00 PRIMER_RIGHT_19_SELF_ANY=6.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=8.500000 PRIMER_LEFT_19_END_STABILITY=9.3000 PRIMER_RIGHT_19_END_STABILITY=6.9000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=2.00 PRIMER_PAIR_19_PRODUCT_SIZE=3045 = primer3-2.3.6/test/primer_task_th_output0000664000077300007730000003477512231156245017433 0ustar ioanaioanaPRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 P3_FILE_FLAG=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_TASK=pick_pcr_primers PRIMER_DNTP_CONC=0.0 SEQUENCE_ID=pcr_primers SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, high hairpin stability 3, ok 0 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=pcr_primers_and_io PRIMER_TASK=pick_pcr_primers_and_hyb_PROBE SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 281, too many Ns 210, low tm 32, high tm 27, ok 12 PRIMER_RIGHT_EXPLAIN=considered 284, too many Ns 210, low tm 11, high tm 38, high hairpin stability 8, ok 17 PRIMER_INTERNAL_EXPLAIN=considered 393, too many Ns 280, low tm 76, high tm 6, long poly-x seq 3, ok 28 PRIMER_PAIR_EXPLAIN=considered 5, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.564009 PRIMER_LEFT_0_PENALTY=2.796318 PRIMER_RIGHT_0_PENALTY=0.767691 PRIMER_INTERNAL_0_PENALTY=0.180037 PRIMER_LEFT_0_SEQUENCE=GCTGTTTCACACTTACGGAA PRIMER_RIGHT_0_SEQUENCE=TGTTGGAGAAAAGCCACTGG PRIMER_INTERNAL_0_SEQUENCE=TTAGCGTGCGTGGGGTCCCT PRIMER_LEFT_0=7,20 PRIMER_RIGHT_0=244,20 PRIMER_INTERNAL_0=142,20 PRIMER_LEFT_0_TM=57.204 PRIMER_RIGHT_0_TM=59.232 PRIMER_INTERNAL_0_TM=60.180 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=65.000 PRIMER_INTERNAL_0_SELF_ANY_TH=5.48 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=35.22 PRIMER_INTERNAL_0_HAIRPIN_TH=34.96 PRIMER_LEFT_0_END_STABILITY=4.3000 PRIMER_RIGHT_0_END_STABILITY=4.0000 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.32 PRIMER_PAIR_0_PRODUCT_SIZE=238 PRIMER_PAIR_1_PENALTY=3.818870 PRIMER_LEFT_1_PENALTY=3.051179 PRIMER_RIGHT_1_PENALTY=0.767691 PRIMER_INTERNAL_1_PENALTY=0.180037 PRIMER_LEFT_1_SEQUENCE=GCTGTTTCACACTTACGGAAA PRIMER_RIGHT_1_SEQUENCE=TGTTGGAGAAAAGCCACTGG PRIMER_INTERNAL_1_SEQUENCE=TTAGCGTGCGTGGGGTCCCT PRIMER_LEFT_1=7,21 PRIMER_RIGHT_1=244,20 PRIMER_INTERNAL_1=142,20 PRIMER_LEFT_1_TM=57.949 PRIMER_RIGHT_1_TM=59.232 PRIMER_INTERNAL_1_TM=60.180 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=65.000 PRIMER_INTERNAL_1_SELF_ANY_TH=5.48 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=35.22 PRIMER_INTERNAL_1_HAIRPIN_TH=34.96 PRIMER_LEFT_1_END_STABILITY=3.1300 PRIMER_RIGHT_1_END_STABILITY=4.0000 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.32 PRIMER_PAIR_1_PRODUCT_SIZE=238 PRIMER_PAIR_2_PENALTY=3.853878 PRIMER_LEFT_2_PENALTY=2.796318 PRIMER_RIGHT_2_PENALTY=1.057560 PRIMER_INTERNAL_2_PENALTY=0.180037 PRIMER_LEFT_2_SEQUENCE=GCTGTTTCACACTTACGGAA PRIMER_RIGHT_2_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_INTERNAL_2_SEQUENCE=TTAGCGTGCGTGGGGTCCCT PRIMER_LEFT_2=7,20 PRIMER_RIGHT_2=247,20 PRIMER_INTERNAL_2=142,20 PRIMER_LEFT_2_TM=57.204 PRIMER_RIGHT_2_TM=58.942 PRIMER_INTERNAL_2_TM=60.180 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=65.000 PRIMER_INTERNAL_2_SELF_ANY_TH=5.48 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=38.11 PRIMER_INTERNAL_2_HAIRPIN_TH=34.96 PRIMER_LEFT_2_END_STABILITY=4.3000 PRIMER_RIGHT_2_END_STABILITY=5.0100 PRIMER_PAIR_2_COMPL_ANY_TH=4.27 PRIMER_PAIR_2_COMPL_END_TH=4.03 PRIMER_PAIR_2_PRODUCT_SIZE=241 PRIMER_PAIR_3_PENALTY=3.857112 PRIMER_LEFT_3_PENALTY=2.796318 PRIMER_RIGHT_3_PENALTY=1.060794 PRIMER_INTERNAL_3_PENALTY=0.180037 PRIMER_LEFT_3_SEQUENCE=GCTGTTTCACACTTACGGAA PRIMER_RIGHT_3_SEQUENCE=CCTGTTGGAGAAAAGCCACT PRIMER_INTERNAL_3_SEQUENCE=TTAGCGTGCGTGGGGTCCCT PRIMER_LEFT_3=7,20 PRIMER_RIGHT_3=246,20 PRIMER_INTERNAL_3=142,20 PRIMER_LEFT_3_TM=57.204 PRIMER_RIGHT_3_TM=58.939 PRIMER_INTERNAL_3_TM=60.180 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_SELF_ANY_TH=5.48 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=35.22 PRIMER_INTERNAL_3_HAIRPIN_TH=34.96 PRIMER_LEFT_3_END_STABILITY=4.3000 PRIMER_RIGHT_3_END_STABILITY=4.0000 PRIMER_PAIR_3_COMPL_ANY_TH=3.04 PRIMER_PAIR_3_COMPL_END_TH=2.28 PRIMER_PAIR_3_PRODUCT_SIZE=240 PRIMER_PAIR_4_PENALTY=4.080246 PRIMER_LEFT_4_PENALTY=3.312555 PRIMER_RIGHT_4_PENALTY=0.767691 PRIMER_INTERNAL_4_PENALTY=0.180037 PRIMER_LEFT_4_SEQUENCE=GCTGTTTCACACTTACGGAAATG PRIMER_RIGHT_4_SEQUENCE=TGTTGGAGAAAAGCCACTGG PRIMER_INTERNAL_4_SEQUENCE=TTAGCGTGCGTGGGGTCCCT PRIMER_LEFT_4=7,23 PRIMER_RIGHT_4=244,20 PRIMER_INTERNAL_4=142,20 PRIMER_LEFT_4_TM=59.687 PRIMER_RIGHT_4_TM=59.232 PRIMER_INTERNAL_4_TM=60.180 PRIMER_LEFT_4_GC_PERCENT=43.478 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=65.000 PRIMER_INTERNAL_4_SELF_ANY_TH=5.48 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=35.22 PRIMER_INTERNAL_4_HAIRPIN_TH=34.96 PRIMER_LEFT_4_END_STABILITY=2.3200 PRIMER_RIGHT_4_END_STABILITY=4.0000 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.32 PRIMER_PAIR_4_PRODUCT_SIZE=238 = SEQUENCE_ID=left_only PRIMER_TASK=pick_left_only SEQUENCE_TARGET=100,20 SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 1121, too many Ns 38, overlap target 23, low tm 34, high tm 809, high hairpin stability 88, ok 129 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.199248 PRIMER_LEFT_0_SEQUENCE=TGCAGGTTTAGAAGGGGTGT PRIMER_LEFT_0=65,20 PRIMER_LEFT_0_TM=59.801 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.1600 PRIMER_LEFT_1_PENALTY=0.243148 PRIMER_LEFT_1_SEQUENCE=CCTGCAGGTTTAGAAGGGGT PRIMER_LEFT_1=63,20 PRIMER_LEFT_1_TM=60.243 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY_TH=26.16 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=4.9500 PRIMER_LEFT_2_PENALTY=0.382831 PRIMER_LEFT_2_SEQUENCE=ATGCCACGTCCATTTGCTTC PRIMER_LEFT_2=146,20 PRIMER_LEFT_2_TM=60.383 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=3.8600 PRIMER_LEFT_3_PENALTY=0.382831 PRIMER_LEFT_3_SEQUENCE=GATGCCACGTCCATTTGCTT PRIMER_LEFT_3=145,20 PRIMER_LEFT_3_TM=60.383 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=3.9100 PRIMER_LEFT_4_PENALTY=0.417433 PRIMER_LEFT_4_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_4=72,20 PRIMER_LEFT_4_TM=59.583 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=4.6100 = SEQUENCE_ID=right_only PRIMER_TASK=PICK_right_only SEQUENCE_TARGET=90,10 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_RIGHT_EXPLAIN=considered 623, too many Ns 27, overlap target 36, GC content failed 5, low tm 279, high tm 102, high hairpin stability 25, ok 149 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.033659 PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAG PRIMER_RIGHT_0=126,20 PRIMER_RIGHT_0_TM=60.034 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_END_STABILITY=3.4600 PRIMER_RIGHT_1_PENALTY=1.023063 PRIMER_RIGHT_1_SEQUENCE=GTGCTTTGCGTTTTGGAAGT PRIMER_RIGHT_1=125,20 PRIMER_RIGHT_1_TM=58.977 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_END_STABILITY=3.0100 PRIMER_RIGHT_2_PENALTY=1.026309 PRIMER_RIGHT_2_SEQUENCE=GAGCGTGCTTTGCGTTTTG PRIMER_RIGHT_2=129,19 PRIMER_RIGHT_2_TM=60.026 PRIMER_RIGHT_2_GC_PERCENT=52.632 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=37.77 PRIMER_RIGHT_2_END_STABILITY=2.4400 PRIMER_RIGHT_3_PENALTY=1.363507 PRIMER_RIGHT_3_SEQUENCE=GTGCTTTGCGTTTTGGAAGTT PRIMER_RIGHT_3=125,21 PRIMER_RIGHT_3_TM=59.636 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_END_STABILITY=2.6600 PRIMER_RIGHT_4_PENALTY=1.754055 PRIMER_RIGHT_4_SEQUENCE=TGCTTTGCGTTTTGGAAGTT PRIMER_RIGHT_4=124,20 PRIMER_RIGHT_4_TM=58.246 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_END_STABILITY=2.6600 = SEQUENCE_ID=hyb_probe_only PRIMER_TASK=pick_HYB_probe_ONly SEQUENCE_INTERNAL_EXCLUDED_REGION=90,20 SEQUENCE_TEMPLATE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_INTERNAL_EXPLAIN=considered 1040, too many Ns 27, overlap excluded region 33, low tm 271, high tm 297, high hairpin stability 141, ok 271 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_INTERNAL_0_PENALTY=0.031870 PRIMER_INTERNAL_0_SEQUENCE=GCTCCTGCCTCTCCGGGACA PRIMER_INTERNAL_0=23,20 PRIMER_INTERNAL_0_TM=59.968 PRIMER_INTERNAL_0_GC_PERCENT=70.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=30.41 PRIMER_INTERNAL_1_PENALTY=0.111561 PRIMER_INTERNAL_1_SEQUENCE=CCGCGGATGCCACGTCCATT PRIMER_INTERNAL_1=140,20 PRIMER_INTERNAL_1_TM=60.112 PRIMER_INTERNAL_1_GC_PERCENT=65.000 PRIMER_INTERNAL_1_SELF_ANY_TH=20.33 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=34.40 PRIMER_INTERNAL_2_PENALTY=0.388807 PRIMER_INTERNAL_2_SEQUENCE=TCCTGCCTCTCCGGGACACC PRIMER_INTERNAL_2=25,20 PRIMER_INTERNAL_2_TM=59.611 PRIMER_INTERNAL_2_GC_PERCENT=70.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=30.41 PRIMER_INTERNAL_3_PENALTY=0.602519 PRIMER_INTERNAL_3_SEQUENCE=CCTGCCTCTCCGGGACACCC PRIMER_INTERNAL_3=26,20 PRIMER_INTERNAL_3_TM=60.603 PRIMER_INTERNAL_3_GC_PERCENT=75.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=38.95 PRIMER_INTERNAL_4_PENALTY=1.037103 PRIMER_INTERNAL_4_SEQUENCE=CCACAGCCAGCTCTGCCCG PRIMER_INTERNAL_4=124,19 PRIMER_INTERNAL_4_TM=60.037 PRIMER_INTERNAL_4_GC_PERCENT=73.684 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=34.17 = SEQUENCE_ID=left_only PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.417433 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_0=0,20 PRIMER_LEFT_0_TM=59.583 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.6100 = SEQUENCE_ID=right_only PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.417433 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_0=0,20 PRIMER_LEFT_0_TM=59.583 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.6100 = SEQUENCE_ID=hyb_probe_only PRIMER_INTERNAL_MIN_SIZE=20 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.417433 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_0=0,20 PRIMER_LEFT_0_TM=59.583 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.6100 = primer3-2.3.6/test/primer_new_tasks_input0000664000077300007730000011203212231156245017552 0ustar ioanaioanaSEQUENCE_ID=Check left only SEQUENCE_PRIMER=gtcctcgaacagctcagtcc PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Check internal only SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Check right only SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick the same primers as before SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=1 = SEQUENCE_ID=Check primers with sequence SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Check primers without sequence PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Check primers without sequence and internal PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick three times the same primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=5 = SEQUENCE_ID=Make a list of a small range SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatga PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=20 = SEQUENCE_ID=Make a list of a small range SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatga PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=2 = SEQUENCE_ID=Check left only too small SEQUENCE_PRIMER=gtcctcgaaca PRIMER_TASK=check_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Check left only too big SEQUENCE_PRIMER=tgacctccacttctctggacacagctccctcaccaa PRIMER_TASK=check_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick sequencing primers no target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=50 = SEQUENCE_ID=Pick sequencing primers small target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=450,37 PRIMER_NUM_RETURN=50 = SEQUENCE_ID=Pick sequencing primers left only SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=150,800 PRIMER_NUM_RETURN=50 = SEQUENCE_ID=Pick sequencing primers right only SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 SEQUENCE_TARGET=150,800 PRIMER_NUM_RETURN=50 = SEQUENCE_ID=Pick sequencing primers three target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=350,37 450,100 850,500 1200,100 PRIMER_NUM_RETURN=50 = SEQUENCE_ID=Pick fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 SEQUENCE_FORCE_LEFT_END=34 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_START=697 SEQUENCE_FORCE_RIGHT_END=678 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick start fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick start fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_START=697 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick end fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_END=34 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick end fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_END=678 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick cloning primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_cloning_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_INCLUDED_REGION=15,203 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick cloning primers with only right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_cloning_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 SEQUENCE_INCLUDED_REGION=15,203 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick discriminative primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_discriminative_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=35,163 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick start fixed left primer unique SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 PRIMER_NUM_RETURN=3 PRIMER_MIN_THREE_PRIME_DISTANCE=0 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick a left primer and an internal oligo SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=1 = SEQUENCE_ID=Pick a right primer and an internal oligo SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=generic PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=1 = SEQUENCE_ID=Test behavior of SEQUENCE_PRIMER_PAIR_OK_REGION_LIST with PRIMER_TASK=pick_primer_list SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg PRIMER_TASK=pick_primer_list PRIMER_NUM_RETURN=15 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=40-100 PRIMER_MIN_GC=0 PRIMER_MIN_TM=0 PRIMER_PICK_ANYWAY=0 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_SIZE=16 SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=1,20,49,17 = primer3-2.3.6/test/dpal_score_output0000664000077300007730000000333212231156245016514 0ustar ioanaioana14.00 12.00 12.00 12.00 1.00 1.00 1.00 1.00 5.00 5.00 5.00 5.00 2.00 -1.00 1.00 0.00 1.00 -1.00 -1.00 0.00 6.00 4.00 6.00 4.00 10.00 6.00 6.00 6.00 1.00 -1.00 -1.00 1.00 1.00 1.00 1.00 1.00 0.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 1.00 1.00 -1.00 -1.00 0.00 14.00 12.00 12.00 12.00 1.00 1.00 1.00 1.00 5.00 5.00 5.00 5.00 2.00 -1.00 1.00 0.00 1.00 -1.00 -1.00 0.00 6.00 4.00 6.00 4.00 10.00 6.00 6.00 6.00 1.00 -1.00 -1.00 1.00 1.00 1.00 1.00 1.00 0.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 1.00 1.00 -1.00 -1.00 0.00 14.00 12.00 12.00 12.00 1.00 1.00 1.00 1.00 5.00 5.00 5.00 5.00 2.00 -1.00 1.00 0.00 1.00 -1.00 -1.00 0.00 6.00 4.00 6.00 4.00 10.00 6.00 6.00 6.00 1.00 -1.00 -1.00 1.00 1.00 1.00 1.00 1.00 0.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 1.00 1.00 -1.00 -1.00 0.00 9.00 5.00 5.00 6.00 1.00 1.00 1.00 1.00 5.00 5.00 5.00 5.00 2.00 -1.00 1.00 0.00 1.00 -1.00 -1.00 0.00 6.00 4.00 6.00 4.00 10.00 6.00 6.00 6.00 1.00 -1.00 -1.00 1.00 1.00 1.00 1.00 1.00 0.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 0.00 1.00 -1.00 -1.00 1.00 1.00 -1.00 -1.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 21.00 21.00 21.00 21.00 16.00 16.00 16.00 16.00 24.00 22.00 22.00 24.00 16.00 16.00 16.00 16.00 11.00 11.00 11.00 11.00 10.00 10.00 10.00 10.00 12.00 12.00 12.00 12.00 10.00 8.00 8.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 primer3-2.3.6/test/primer_num_best_input0000664000077300007730000001666212231156245017404 0ustar ioanaioanaPRIMER_NUM_RETURN=2 PRIMER_OPT_SIZE=20 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000 PRIMER_MAX_GC=80.0 PRIMER_MAX_SIZE=25 PRIMER_MAX_TM=63.0 PRIMER_MIN_SIZE=18 PRIMER_MIN_GC=20 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=6 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=6 SEQUENCE_ID=01.RHAP33FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,315 SEQUENCE_TARGET=81,30 = SEQUENCE_ID=01.RHAP34FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT SEQUENCE_INCLUDED_REGION=19,296 SEQUENCE_TARGET=134,15 SEQUENCE_TARGET=213,16 SEQUENCE_TARGET=235,16 = PRIMER_NUM_RETURN=1 SEQUENCE_ID=03.RHAP34FA3.seq SEQUENCE_TEMPLATE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT SEQUENCE_INCLUDED_REGION=0,278 SEQUENCE_TARGET=1,28 = SEQUENCE_ID=03.RHAP34FE3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA SEQUENCE_INCLUDED_REGION=17,284 SEQUENCE_TARGET=27,60 = SEQUENCE_ID=04.RHAP33FE4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC SEQUENCE_INCLUDED_REGION=18,367 SEQUENCE_TARGET=146,46 SEQUENCE_TARGET=199,22 SEQUENCE_TARGET=286,13 SEQUENCE_TARGET=300,15 = SEQUENCE_ID=05.RHAP33FA5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA SEQUENCE_INCLUDED_REGION=10,369 SEQUENCE_TARGET=30,44 = SEQUENCE_ID=05.RHAP33FE5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,306 SEQUENCE_TARGET=176,34 = PRIMER_NUM_RETURN=40 SEQUENCE_ID=05.RHAP34FA5.seq SEQUENCE_TEMPLATE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA SEQUENCE_INCLUDED_REGION=9,297 SEQUENCE_TARGET=125,36 SEQUENCE_TARGET=162,24 SEQUENCE_TARGET=191,16 SEQUENCE_TARGET=214,14 = SEQUENCE_ID=06.RHAP34FE6.seq SEQUENCE_TEMPLATE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA SEQUENCE_INCLUDED_REGION=8,287 SEQUENCE_TARGET=61,68 = SEQUENCE_ID=07.RHAP34FE7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT SEQUENCE_INCLUDED_REGION=18,283 SEQUENCE_TARGET=115,29 SEQUENCE_TARGET=145,20 SEQUENCE_TARGET=166,24 = SEQUENCE_ID=08.RHAP34FE8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG SEQUENCE_INCLUDED_REGION=20,281 SEQUENCE_TARGET=119,62 = PRIMER_NUM_RETURN=8 SEQUENCE_ID=09.RHAP33FA9.seq SEQUENCE_TEMPLATE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG SEQUENCE_INCLUDED_REGION=0,371 SEQUENCE_TARGET=151,18 SEQUENCE_TARGET=192,53 SEQUENCE_TARGET=248,16 = SEQUENCE_ID=09.RHAP34FA9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT SEQUENCE_INCLUDED_REGION=20,280 SEQUENCE_TARGET=44,42 = SEQUENCE_ID=10.RHAP34FA10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=12,236 SEQUENCE_TARGET=155,32 = SEQUENCE_ID=13.RHAP34FB1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,244 SEQUENCE_TARGET=22,16 SEQUENCE_TARGET=57,34 = SEQUENCE_ID=14.RHAP34FB2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC SEQUENCE_INCLUDED_REGION=13,303 SEQUENCE_TARGET=177,33 SEQUENCE_TARGET=254,14 = primer3-2.3.6/test/primer_new_tasks_formatted_output0000664000077300007730000027331712231156245022036 0ustar ioanaioanaPRIMER PICKING RESULTS FOR Check left only No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 20 59.99 60.00 4.00 2.00 gtcctcgaacagctcagtcc SEQUENCE SIZE: 200 INCLUDED REGION SIZE: 200 0 gtcctcgaacagctcagtccNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN >>>>>>>>>>>>>>>>>>>> 60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 180 NNNNNNNNNNNNNNNNNNNN KEYS (in order of precedence): >>>>>> left primer PRIMER PICKING RESULTS FOR Check internal only No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq INTERNAL_OLIGO 90 20 60.19 50.00 2.00 0.00 ccacggatgaggaagaagaa SEQUENCE SIZE: 200 INCLUDED REGION SIZE: 200 0 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNccacggatgaggaagaagaaNNNNNNNNNN ^^^^^^^^^^^^^^^^^^^^ 120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 180 NNNNNNNNNNNNNNNNNNNN KEYS (in order of precedence): ^^^^^^ internal oligo PRIMER PICKING RESULTS FOR Check right only No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq RIGHT_PRIMER 199 20 60.01 40.00 2.00 0.00 gcgtttttattgccgttgtt SEQUENCE SIZE: 200 INCLUDED REGION SIZE: 200 0 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 180 aacaacggcaataaaaacgc <<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): <<<<<< right primer PRIMER PICKING RESULTS FOR Pick the same primers as before No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 198 20 59.99 60.00 4.00 2.00 gtcctcgaacagctcagtcc RIGHT PRIMER 315 20 60.01 40.00 2.00 0.00 gcgtttttattgccgttgtt INTERNAL OLIGO 250 20 60.19 50.00 2.00 0.00 ccacggatgaggaagaagaa SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 PRODUCT SIZE: 118, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc >>>>>>>>>>>>>>>>>>>> 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca ^^^^^^^^^^^^^^^^^^^^ <<<< 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta <<<<<<<<<<<<<<<< 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo PRIMER PICKING RESULTS FOR Check primers with sequence No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 198 20 59.99 60.00 4.00 2.00 gtcctcgaacagctcagtcc RIGHT PRIMER 315 20 60.01 40.00 2.00 0.00 gcgtttttattgccgttgtt INTERNAL OLIGO 250 20 60.19 50.00 2.00 0.00 ccacggatgaggaagaagaa SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 PRODUCT SIZE: 118, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc >>>>>>>>>>>>>>>>>>>> 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca ^^^^^^^^^^^^^^^^^^^^ <<<< 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta <<<<<<<<<<<<<<<< 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo PRIMER PICKING RESULTS FOR Check primers without sequence No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 20 59.99 60.00 4.00 2.00 gtcctcgaacagctcagtcc RIGHT PRIMER 199 20 60.01 40.00 2.00 0.00 gcgtttttattgccgttgtt INTERNAL OLIGO 90 20 60.19 50.00 2.00 0.00 ccacggatgaggaagaagaa SEQUENCE SIZE: 200 INCLUDED REGION SIZE: 200 PRODUCT SIZE: 200, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 0 gtcctcgaacagctcagtccNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN >>>>>>>>>>>>>>>>>>>> 60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNccacggatgaggaagaagaaNNNNNNNNNN ^^^^^^^^^^^^^^^^^^^^ 120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 180 aacaacggcaataaaaacgc <<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo PRIMER PICKING RESULTS FOR Check primers without sequence and internal No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 20 59.99 60.00 4.00 2.00 gtcctcgaacagctcagtcc RIGHT PRIMER 199 20 60.01 40.00 2.00 0.00 gcgtttttattgccgttgtt SEQUENCE SIZE: 200 INCLUDED REGION SIZE: 200 PRODUCT SIZE: 200, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 0 gtcctcgaacagctcagtccNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN >>>>>>>>>>>>>>>>>>>> 60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 180 aacaacggcaataaaaacgc <<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer PRIMER PICKING RESULTS FOR Pick three times the same primers No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 start len tm gc% any 3' seq 1 LEFT_PRIMER 1471 20 60.00 50.00 4.00 0.00 gacgtttgctttctgcttcc 2 LEFT_PRIMER 849 20 60.00 50.00 4.00 0.00 atcgccaccgtacaagaaac 3 LEFT_PRIMER 296 20 60.01 40.00 2.00 2.00 aacaacggcaataaaaacgc 4 LEFT_PRIMER 198 20 59.99 60.00 4.00 2.00 gtcctcgaacagctcagtcc 5 LEFT_PRIMER 39 20 59.99 55.00 4.00 0.00 ttctctggacacagctccct start len tm gc% any 3' seq 1 INTERNAL_OLIGO 1471 20 60.00 50.00 4.00 0.00 gacgtttgctttctgcttcc 2 INTERNAL_OLIGO 849 20 60.00 50.00 4.00 0.00 atcgccaccgtacaagaaac 3 INTERNAL_OLIGO 296 20 60.01 40.00 2.00 2.00 aacaacggcaataaaaacgc 4 INTERNAL_OLIGO 198 20 59.99 60.00 4.00 2.00 gtcctcgaacagctcagtcc 5 INTERNAL_OLIGO 39 20 59.99 55.00 4.00 0.00 ttctctggacacagctccct start len tm gc% any 3' seq 1 RIGHT_PRIMER 1490 20 60.00 50.00 4.00 2.00 ggaagcagaaagcaaacgtc 2 RIGHT_PRIMER 868 20 60.00 50.00 4.00 2.00 gtttcttgtacggtggcgat 3 RIGHT_PRIMER 315 20 60.01 40.00 2.00 0.00 gcgtttttattgccgttgtt 4 RIGHT_PRIMER 217 20 59.99 60.00 4.00 2.00 ggactgagctgttcgaggac 5 RIGHT_PRIMER 58 20 59.99 55.00 4.00 0.00 agggagctgtgtccagagaa PRIMER PICKING RESULTS FOR Make a list of a small range No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions SEQUENCE SIZE: 32 INCLUDED REGION SIZE: 32 start len tm gc% any 3' seq 1 LEFT_PRIMER 3 20 58.72 45.00 4.00 2.00 gatttgatggacatggatgc 2 LEFT_PRIMER 1 20 58.56 40.00 4.00 2.00 tggatttgatggacatggat 3 LEFT_PRIMER 0 20 58.56 40.00 4.00 2.00 atggatttgatggacatgga 4 LEFT_PRIMER 0 21 58.93 38.10 4.00 2.00 atggatttgatggacatggat 5 LEFT_PRIMER 1 19 58.16 42.11 4.00 2.00 tggatttgatggacatgga 6 LEFT_PRIMER 2 21 62.41 47.62 4.00 2.00 ggatttgatggacatggatgc start len tm gc% any 3' seq 1 INTERNAL_OLIGO 12 20 60.30 45.00 11.00 9.00 gacatggatgccatcaatga 2 INTERNAL_OLIGO 4 20 60.56 45.00 4.00 4.00 atttgatggacatggatgcc 3 INTERNAL_OLIGO 3 20 58.72 45.00 4.00 2.00 gatttgatggacatggatgc 4 INTERNAL_OLIGO 5 19 60.28 47.37 4.00 4.00 tttgatggacatggatgcc 5 INTERNAL_OLIGO 8 19 59.67 52.63 10.00 10.00 gatggacatggatgccatc 6 INTERNAL_OLIGO 2 20 58.56 45.00 4.00 4.00 ggatttgatggacatggatg 7 INTERNAL_OLIGO 1 20 58.56 40.00 4.00 2.00 tggatttgatggacatggat 8 INTERNAL_OLIGO 0 20 58.56 40.00 4.00 2.00 atggatttgatggacatgga 9 INTERNAL_OLIGO 9 19 60.74 47.37 8.00 5.00 atggacatggatgccatca 10 INTERNAL_OLIGO 7 19 60.74 47.37 8.00 8.00 tgatggacatggatgccat 11 INTERNAL_OLIGO 11 19 59.11 47.37 9.00 9.00 ggacatggatgccatcaat 12 INTERNAL_OLIGO 0 21 58.93 38.10 4.00 2.00 atggatttgatggacatggat 13 INTERNAL_OLIGO 11 20 62.13 50.00 11.00 11.00 ggacatggatgccatcaatg 14 INTERNAL_OLIGO 10 20 62.13 45.00 9.00 9.00 tggacatggatgccatcaat 15 INTERNAL_OLIGO 9 20 62.13 45.00 8.00 7.00 atggacatggatgccatcaa 16 INTERNAL_OLIGO 6 20 62.13 45.00 8.00 8.00 ttgatggacatggatgccat 17 INTERNAL_OLIGO 10 18 60.45 50.00 7.00 5.00 tggacatggatgccatca 18 INTERNAL_OLIGO 7 18 60.45 50.00 6.00 6.00 tgatggacatggatgcca 19 INTERNAL_OLIGO 1 21 61.53 42.86 4.00 4.00 tggatttgatggacatggatg 20 INTERNAL_OLIGO 8 20 62.76 50.00 10.00 6.00 gatggacatggatgccatca start len tm gc% any 3' seq 1 RIGHT_PRIMER 23 20 60.56 45.00 4.00 2.00 ggcatccatgtccatcaaat 2 RIGHT_PRIMER 22 20 58.72 45.00 4.00 0.00 gcatccatgtccatcaaatc 3 RIGHT_PRIMER 23 19 60.28 47.37 4.00 0.00 ggcatccatgtccatcaaa 4 RIGHT_PRIMER 21 20 58.56 45.00 4.00 0.00 catccatgtccatcaaatcc 5 RIGHT_PRIMER 20 20 58.56 40.00 4.00 0.00 atccatgtccatcaaatcca 6 RIGHT_PRIMER 19 20 58.56 40.00 4.00 2.00 tccatgtccatcaaatccat 7 RIGHT_PRIMER 25 19 60.74 47.37 8.00 1.00 atggcatccatgtccatca 8 RIGHT_PRIMER 20 21 58.93 38.10 4.00 2.00 atccatgtccatcaaatccat 9 RIGHT_PRIMER 25 20 62.13 45.00 8.00 2.00 atggcatccatgtccatcaa 10 RIGHT_PRIMER 24 18 60.45 50.00 6.00 1.00 tggcatccatgtccatca 11 RIGHT_PRIMER 21 21 61.53 42.86 4.00 0.00 catccatgtccatcaaatcca 12 RIGHT_PRIMER 19 19 58.16 42.11 4.00 0.00 tccatgtccatcaaatcca 13 RIGHT_PRIMER 24 19 61.95 47.37 6.00 2.00 tggcatccatgtccatcaa 14 RIGHT_PRIMER 23 18 58.72 50.00 4.00 2.00 ggcatccatgtccatcaa 15 RIGHT_PRIMER 23 21 62.41 47.62 4.00 0.00 ggcatccatgtccatcaaatc 16 RIGHT_PRIMER 22 21 62.41 47.62 4.00 0.00 gcatccatgtccatcaaatcc 17 RIGHT_PRIMER 21 22 61.72 40.91 4.00 2.00 catccatgtccatcaaatccat PRIMER PICKING RESULTS FOR Make a list of a small range No mispriming library specified Using 0-based sequence positions SEQUENCE SIZE: 32 INCLUDED REGION SIZE: 32 start len tm gc% any 3' seq 1 RIGHT_PRIMER 23 20 60.56 45.00 4.00 2.00 ggcatccatgtccatcaaat 2 RIGHT_PRIMER 22 20 58.72 45.00 4.00 0.00 gcatccatgtccatcaaatc PRIMER PICKING RESULTS FOR Check left only too small No mispriming library specified Using 0-based sequence positions WARNING: Specified left primer < PRIMER_MIN_SIZE; Left primer is unacceptable: Too short/Tm too low OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 11 27.26 54.55 4.00 2.00 gtcctcgaaca SEQUENCE SIZE: 200 INCLUDED REGION SIZE: 200 0 gtcctcgaacaNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN >>>>>>>>>>> 60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 180 NNNNNNNNNNNNNNNNNNNN KEYS (in order of precedence): >>>>>> left primer PRIMER PICKING RESULTS FOR Check left only too big No mispriming library specified Using 0-based sequence positions WARNING: Specified left primer > PRIMER_MAX_SIZE; Left primer is unacceptable: Too long/Tm too high OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 36 80.81 55.56 4.00 0.00 tgacctccacttctctggacacagctccctcaccaa SEQUENCE SIZE: 200 INCLUDED REGION SIZE: 200 0 tgacctccacttctctggacacagctccctcaccaaNNNNNNNNNNNNNNNNNNNNNNNN >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> 60 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 180 NNNNNNNNNNNNNNNNNNNN KEYS (in order of precedence): >>>>>> left primer PRIMER PICKING RESULTS FOR Pick sequencing primers no target No mispriming library specified Using 0-based sequence positions SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 TARGETS (start, len)*: 0,1527 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc *******************>>>>>>>>>>>>>>>>>>>********************** 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc ************************************************************ 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc ************************************************************ 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc ******************<<<<<<<<<<<<<<<<<<<<********************** 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca ************************************************************ 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta ************>>>>>>>>>>>>>>>>>>>>**************************** 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg ************************************************************ 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga ************************************************************ 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat ************************************************************ 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac ************************************************************ 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa ************************************************************ 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc ******************<<<<<<<<<<<<<<<<<<<<********************** 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt ************************************************************ 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc *********************>>>>>>>>>>>>>>>>>>>>******************* 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc ************************************************************ 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct ************************************************************ 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct ************************************************************ 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg ************************************************************ 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg ************************************************************ 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt ********************************************<<<<<<<<<<<<<<<< 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt <<<<******************************************************** 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg *********************************************************>>> 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa >>>>>>>>>>>>>>>>>******************************************* 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt ************************************************************ 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact *************************************************<<<<<<<<<<< 1500 tgttcttcttcagattctgatttatag <<<<<<<<<<<**************** KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ^^^^^^ left primer / right primer overlap start len tm gc% any 3' seq 1 LEFT_PRIMER 19 19 59.87 47.37 5.00 0.00 atgccatcaatgacctcca 2 LEFT_PRIMER 312 20 60.12 45.00 4.00 2.00 acgcatgaagttggtgatga 3 LEFT_PRIMER 801 20 59.92 45.00 6.00 2.00 catcttcggataaccgcatt 4 LEFT_PRIMER 1317 20 60.23 60.00 2.00 0.00 ggggagtggttaggtgaggt start len tm gc% any 3' seq 1 RIGHT_PRIMER 217 20 59.99 60.00 4.00 2.00 ggactgagctgttcgaggac 2 RIGHT_PRIMER 697 20 60.19 45.00 4.00 1.00 gcttcgatgatggcttgatt 3 RIGHT_PRIMER 1203 20 60.05 45.00 3.00 3.00 gaaaaccagcctccaatgaa 4 RIGHT_PRIMER 1510 22 59.26 45.45 2.00 0.00 gaagaagaacaagtccaaaggg PRIMER PICKING RESULTS FOR Pick sequencing primers small target No mispriming library specified Using 0-based sequence positions SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 TARGETS (start, len)*: 450,37 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca >>>>>>>>>>>>>>>> 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta >>>> 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga ****************************** 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat ******* 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa <<<<<<<<< 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc <<<<<<<<<<< 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ^^^^^^ left primer / right primer overlap start len tm gc% any 3' seq 1 LEFT_PRIMER 284 20 59.36 45.00 2.00 1.00 aaccacaacaacaacaacgg start len tm gc% any 3' seq 1 RIGHT_PRIMER 670 20 60.50 45.00 4.00 2.00 aagtggccgaatttgacgta PRIMER PICKING RESULTS FOR Pick sequencing primers left only No mispriming library specified Using 0-based sequence positions SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 TARGETS (start, len)*: 150,800 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc >>>>>>>>>>>>>>>>>>> 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc ****************************** 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc ************************************************************ 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca ************************************************************ 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta ************************************************************ 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg ************************************************************ 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga *********************************************************>>> 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat >>>>>>>>>>>>>>>>>******************************************* 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac ************************************************************ 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa ************************************************************ 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc ************************************************************ 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt ************************************************************ 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc ************************************************************ 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc ************************************************************ 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct ************************************************** 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ^^^^^^ left primer / right primer overlap start len tm gc% any 3' seq 1 LEFT_PRIMER 19 19 59.87 47.37 5.00 0.00 atgccatcaatgacctcca 2 LEFT_PRIMER 477 20 60.41 50.00 3.00 0.00 agacttgccgctcatttcac PRIMER PICKING RESULTS FOR Pick sequencing primers right only No mispriming library specified Using 0-based sequence positions SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 TARGETS (start, len)*: 150,800 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc ****************************** 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc ************************************************************ 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca ************************************************************ 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta ************************************************************ 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg ************************************************************ 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga ************************************************************ 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat ************************************************************ 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac ************************************************************ 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa *********<<<<<<<<<<<<<<<<<<<<******************************* 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc ************************************************************ 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt ************************************************************ 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc ************************************************************ 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc ************************************************************ 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct ************************************************** 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg <<<<<<<<<<<<<<<<<<<< 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ^^^^^^ left primer / right primer overlap start len tm gc% any 3' seq 1 RIGHT_PRIMER 628 20 60.03 50.00 3.00 1.00 tggaaagcagcaagtgtgtc 2 RIGHT_PRIMER 1107 20 59.34 50.00 4.00 2.00 cttcaaccaacgtcacaagc PRIMER PICKING RESULTS FOR Pick sequencing primers three target No mispriming library specified Using 0-based sequence positions SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 TARGETS (start, len)*: 350,37 450,100 850,500 1200,100 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc >>>>> 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc >>>>>>>>>>>>>>> 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca >>>> 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta >>>>>>>>>>>>>>>> ********** 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg *************************** 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga ****************************** 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat ************************************************************ 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac ***<<<<<<<<<<<<<<<<<<<< 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc >>>>>>>>>>>^^^^^^^^^<<<<<<<<<<< 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc ************************************************** 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct ************************************************************ 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct ************************************************************ 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg *<<<<<<<<<<<<<<<<<<^^>>>>>>>>>>>>>>>>>>********************* 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg ************************************************************ 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt ********************************>>>>>>>>>>>>>>>>>>>>******** 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt ************************************************************ 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg ************************************************************ 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa ****************************** 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt <<<<<<<<<<<<<<<<<<<< 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact <<<<<<<<<<< 1500 tgttcttcttcagattctgatttatag <<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ^^^^^^ left primer / right primer overlap start len tm gc% any 3' seq 1 LEFT_PRIMER 175 20 59.63 50.00 6.00 0.00 ccatcatcgaggacagaaca 2 LEFT_PRIMER 296 20 60.01 40.00 2.00 2.00 aacaacggcaataaaaacgc 3 LEFT_PRIMER 667 20 60.26 50.00 3.00 1.00 acttcacagccaatcaagcc 4 LEFT_PRIMER 1172 20 59.94 40.00 5.00 1.00 agcggtttttgattcattgg 5 LEFT_PRIMER 1039 20 60.25 45.00 6.00 0.00 cggaatcagttgcttcgttt start len tm gc% any 3' seq 1 RIGHT_PRIMER 562 20 59.70 45.00 2.00 0.00 ttgttgtggtgatggtggtt 2 RIGHT_PRIMER 697 20 60.19 45.00 4.00 1.00 gcttcgatgatggcttgatt 3 RIGHT_PRIMER 1040 20 60.05 55.00 6.00 3.00 cggtgcacggtaactaaggt 4 RIGHT_PRIMER 1510 22 59.26 45.45 2.00 0.00 gaagaagaacaagtccaaaggg 5 RIGHT_PRIMER 1434 20 59.96 55.00 3.00 2.00 cacccacctcttcaacccta PRIMER PICKING RESULTS FOR Pick fixed left primer No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 15 20 60.17 45.00 9.00 4.00 atggatgccatcaatgacct RIGHT PRIMER 217 20 59.99 60.00 4.00 2.00 ggactgagctgttcgaggac SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc >>>>>>>>>>>>>>>>>>>> 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc <<<<<<<<<<<<<<<<<<<< 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 15 20 60.17 45.00 9.00 4.00 atggatgccatcaatgacct RIGHT PRIMER 125 20 59.97 55.00 3.00 2.00 gttgacgatgggagaccagt PRODUCT SIZE: 111, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 15 20 60.17 45.00 9.00 4.00 atggatgccatcaatgacct RIGHT PRIMER 126 20 60.11 55.00 3.00 1.00 agttgacgatgggagaccag PRODUCT SIZE: 112, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 PRIMER PICKING RESULTS FOR Pick fixed right primer No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 596 20 60.05 50.00 2.00 0.00 cgacaaccaaaacgacacac RIGHT PRIMER 697 20 60.19 45.00 4.00 1.00 gcttcgatgatggcttgatt SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 PRODUCT SIZE: 102, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac >>>> 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa >>>>>>>>>>>>>>>> 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc <<<<<<<<<<<<<<<<<<<< 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 595 20 60.05 45.00 2.00 0.00 acgacaaccaaaacgacaca RIGHT PRIMER 697 20 60.19 45.00 4.00 1.00 gcttcgatgatggcttgatt PRODUCT SIZE: 103, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 594 20 60.05 50.00 2.00 0.00 cacgacaaccaaaacgacac RIGHT PRIMER 697 20 60.19 45.00 4.00 1.00 gcttcgatgatggcttgatt PRODUCT SIZE: 104, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 PRIMER PICKING RESULTS FOR Pick start fixed left primer No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 15 20 60.17 45.00 9.00 4.00 atggatgccatcaatgacct RIGHT PRIMER 217 20 59.99 60.00 4.00 2.00 ggactgagctgttcgaggac SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc >>>>>>>>>>>>>>>>>>>> 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc <<<<<<<<<<<<<<<<<<<< 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 15 20 60.17 45.00 9.00 4.00 atggatgccatcaatgacct RIGHT PRIMER 125 20 59.97 55.00 3.00 2.00 gttgacgatgggagaccagt PRODUCT SIZE: 111, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 15 20 60.17 45.00 9.00 4.00 atggatgccatcaatgacct RIGHT PRIMER 126 20 60.11 55.00 3.00 1.00 agttgacgatgggagaccag PRODUCT SIZE: 112, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 PRIMER PICKING RESULTS FOR Pick start fixed right primer No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 596 20 60.05 50.00 2.00 0.00 cgacaaccaaaacgacacac RIGHT PRIMER 697 20 60.19 45.00 4.00 1.00 gcttcgatgatggcttgatt SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 PRODUCT SIZE: 102, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac >>>> 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa >>>>>>>>>>>>>>>> 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc <<<<<<<<<<<<<<<<<<<< 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 595 20 60.05 45.00 2.00 0.00 acgacaaccaaaacgacaca RIGHT PRIMER 697 20 60.19 45.00 4.00 1.00 gcttcgatgatggcttgatt PRODUCT SIZE: 103, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 594 20 60.05 50.00 2.00 0.00 cacgacaaccaaaacgacac RIGHT PRIMER 697 20 60.19 45.00 4.00 1.00 gcttcgatgatggcttgatt PRODUCT SIZE: 104, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 PRIMER PICKING RESULTS FOR Pick end fixed left primer No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 15 20 60.17 45.00 9.00 4.00 atggatgccatcaatgacct RIGHT PRIMER 217 20 59.99 60.00 4.00 2.00 ggactgagctgttcgaggac SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc >>>>>>>>>>>>>>>>>>>> 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc <<<<<<<<<<<<<<<<<<<< 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 15 20 60.17 45.00 9.00 4.00 atggatgccatcaatgacct RIGHT PRIMER 125 20 59.97 55.00 3.00 2.00 gttgacgatgggagaccagt PRODUCT SIZE: 111, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 PRIMER PICKING RESULTS FOR Pick end fixed right primer No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 596 20 60.05 50.00 2.00 0.00 cgacaaccaaaacgacacac RIGHT PRIMER 697 20 60.19 45.00 4.00 1.00 gcttcgatgatggcttgatt SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 PRODUCT SIZE: 102, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac >>>> 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa >>>>>>>>>>>>>>>> 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc <<<<<<<<<<<<<<<<<<<< 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 595 20 60.05 45.00 2.00 0.00 acgacaaccaaaacgacaca RIGHT PRIMER 697 20 60.19 45.00 4.00 1.00 gcttcgatgatggcttgatt PRODUCT SIZE: 103, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 PRIMER PICKING RESULTS FOR Pick cloning primers No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 15 20 60.17 45.00 9.00 4.00 atggatgccatcaatgacct RIGHT PRIMER 217 20 59.99 60.00 4.00 2.00 ggactgagctgttcgaggac SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 203 PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc ...............>>>>>>>>>>>>>>>>>>>> 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc <<<<<<<<<<<<<<<<<<<<...................... 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca ............................................................ 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta ............................................................ 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg ............................................................ 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga ............................................................ 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat ............................................................ 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac ............................................................ 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa ............................................................ 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc ............................................................ 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt ............................................................ 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc ............................................................ 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc ............................................................ 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct ............................................................ 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct ............................................................ 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg ............................................................ 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg ............................................................ 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt ............................................................ 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt ............................................................ 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg ............................................................ 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa ............................................................ 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt ............................................................ 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact ............................................................ 1500 tgttcttcttcagattctgatttatag ........................... KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 15 19 59.11 47.37 9.00 2.00 atggatgccatcaatgacc RIGHT PRIMER 217 20 59.99 60.00 4.00 2.00 ggactgagctgttcgaggac PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00 PRIMER PICKING RESULTS FOR Pick cloning primers with only right primer No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq RIGHT_PRIMER 217 20 59.99 60.00 4.00 2.00 ggactgagctgttcgaggac SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 203 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc ............... 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc <<<<<<<<<<<<<<<<<<<<...................... 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca ............................................................ 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta ............................................................ 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg ............................................................ 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga ............................................................ 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat ............................................................ 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac ............................................................ 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa ............................................................ 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc ............................................................ 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt ............................................................ 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc ............................................................ 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc ............................................................ 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct ............................................................ 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct ............................................................ 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg ............................................................ 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg ............................................................ 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt ............................................................ 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt ............................................................ 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg ............................................................ 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa ............................................................ 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt ............................................................ 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact ............................................................ 1500 tgttcttcttcagattctgatttatag ........................... KEYS (in order of precedence): ...... vector sequence <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 RIGHT_PRIMER 217 19 59.06 57.89 4.00 2.00 ggactgagctgttcgagga PRIMER PICKING RESULTS FOR Pick discriminative primers No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 16 20 61.87 50.00 7.00 6.00 tggatgccatcaatgacctc RIGHT PRIMER 216 20 59.13 55.00 4.00 4.00 gactgagctgttcgaggaca SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 PRODUCT SIZE: 201, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 5.00 TARGETS (start, len)*: 35,163 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc >>>>>>>>>>>>>>>>>>>)************************ 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc ************************************************************ 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc ************************************************************ 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc *****************(<<<<<<<<<<<<<<<<<<< 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 17 19 58.82 52.63 7.00 6.00 ggatgccatcaatgacctc RIGHT PRIMER 216 20 59.13 55.00 4.00 4.00 gactgagctgttcgaggaca PRODUCT SIZE: 200, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 5.00 PRIMER PICKING RESULTS FOR Pick start fixed left primer unique No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 15 20 60.17 45.00 9.00 4.00 atggatgccatcaatgacct RIGHT PRIMER 217 20 59.99 60.00 4.00 2.00 ggactgagctgttcgaggac SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 PRODUCT SIZE: 203, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc >>>>>>>>>>>>>>>>>>>> 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc <<<<<<<<<<<<<<<<<<<< 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 15 19 59.11 47.37 9.00 2.00 atggatgccatcaatgacc RIGHT PRIMER 125 20 59.97 55.00 3.00 2.00 gttgacgatgggagaccagt PRODUCT SIZE: 111, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 15 21 62.05 47.62 9.00 6.00 atggatgccatcaatgacctc RIGHT PRIMER 126 20 60.11 55.00 3.00 1.00 agttgacgatgggagaccag PRODUCT SIZE: 112, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 PRIMER PICKING RESULTS FOR Pick a left primer and an internal oligo No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 1471 20 60.00 50.00 4.00 0.00 gacgtttgctttctgcttcc INTERNAL_OLIGO 1471 20 60.00 50.00 4.00 0.00 gacgtttgctttctgcttcc SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact >>>>>>>>>>>>>>>>>>>> 1500 tgttcttcttcagattctgatttatag KEYS (in order of precedence): >>>>>> left primer ADDITIONAL OLIGOS start len tm gc% any 3' seq ADDITIONAL OLIGOS start len tm gc% any 3' seq PRIMER PICKING RESULTS FOR Pick a right primer and an internal oligo No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq INTERNAL_OLIGO 1471 20 60.00 50.00 4.00 0.00 gacgtttgctttctgcttcc RIGHT_PRIMER 1490 20 60.00 50.00 4.00 2.00 ggaagcagaaagcaaacgtc SEQUENCE SIZE: 1527 INCLUDED REGION SIZE: 1527 0 atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctc 60 accaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatc 120 gtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatc 180 atcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccacc 240 accaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaaca 300 acggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcaccta 360 ttgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtg 420 atattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagaga 480 cttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcat 540 aataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgac 600 aaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaa 660 ttcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtc 720 catgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcactt 780 gcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggc 840 cggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttcc 900 ctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtcct 960 tctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacct 1020 caccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttg 1080 aatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtg 1140 gaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggt 1200 tttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaatt 1260 gcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggg 1320 gagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaa 1380 gcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggt 1440 agtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggact <<<<<<<<<<<<<<<<<<<<^^^^^^^^^ 1500 tgttcttcttcagattctgatttatag ^^^^^^^^^^ KEYS (in order of precedence): <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq ADDITIONAL OLIGOS start len tm gc% any 3' seq PRIMER PICKING RESULTS FOR Test behavior of SEQUENCE_PRIMER_PAIR_OK_REGION_LIST with PRIMER_TASK=pick_primer_list No mispriming library specified Using 1-based sequence positions SEQUENCE SIZE: 100 INCLUDED REGION SIZE: 100 PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 1,20,49,17 start len tm gc% any 3' seq 1 LEFT_PRIMER 1 20 51.06 30.00 11.00 5.00 cttgattcaatgaatctcaa 2 LEFT_PRIMER 2 19 49.45 26.32 11.00 5.00 ttgattcaatgaatctcaa 3 LEFT_PRIMER 1 19 48.92 31.58 11.00 7.00 cttgattcaatgaatctca 4 LEFT_PRIMER 3 18 47.09 27.78 11.00 3.00 tgattcaatgaatctcaa 5 LEFT_PRIMER 2 18 47.09 27.78 11.00 7.00 ttgattcaatgaatctca 6 LEFT_PRIMER 1 18 45.15 33.33 11.00 7.00 cttgattcaatgaatctc 7 LEFT_PRIMER 3 17 44.38 29.41 11.00 5.00 tgattcaatgaatctca 8 LEFT_PRIMER 4 17 43.02 29.41 11.00 2.00 gattcaatgaatctcaa 9 LEFT_PRIMER 2 17 43.02 29.41 11.00 7.00 ttgattcaatgaatctc 10 LEFT_PRIMER 1 17 42.26 29.41 11.00 9.00 cttgattcaatgaatct 11 LEFT_PRIMER 4 16 39.89 31.25 11.00 4.00 gattcaatgaatctca 12 LEFT_PRIMER 3 16 39.89 31.25 11.00 7.00 tgattcaatgaatctc 13 LEFT_PRIMER 5 16 39.85 25.00 9.00 2.00 attcaatgaatctcaa 14 LEFT_PRIMER 2 16 39.85 25.00 11.00 9.00 ttgattcaatgaatct 15 LEFT_PRIMER 1 16 39.85 31.25 11.00 11.00 cttgattcaatgaatc start len tm gc% any 3' seq 1 RIGHT_PRIMER 65 17 45.95 29.41 2.00 1.00 aaaaaaacacaatccAg 2 RIGHT_PRIMER 65 16 43.92 25.00 2.00 0.00 aaaaaaacacaatccA 3 RIGHT_PRIMER 64 16 43.31 31.25 2.00 1.00 aaaaaacacaatccAg Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 954 0 0 0 939 0 0 0 0 0 0 0 0 15 Right 954 0 0 0 951 0 0 0 0 0 0 0 0 3 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.3 primer3-2.3.6/test/test_left_to_right_of_right_output0000664000077300007730000000461412231156245022156 0ustar ioanaioanaP3_COMMENT=The SEQUENCE_PRIMER and SEQUENCE_PRIMER_REVCOMP are reverse compliments of each other, and each is found at two positions in the template. The causes there to be 4 pairs to be considered, including one in which the left primer it to the right of the right primer. SEQUENCE_TEMPLATE=AGGCTAGGCGAGCTGAAAAATCCTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGCTAGGCGAGCTGAAAAATCCTAC PRIMER_MAX_TEMPLATE_MISPRIMING=12 PRIMER_PICK_ANYWAY=1 PRIMER_PRODUCT_SIZE_RANGE=30-100 PRIMER_GC_CLAMP=0 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_SIZE=19 PRIMER_TASK=pick_pcr_primers PRIMER_MIN_GC=18 PRIMER_MAX_NS_ACCEPTED=1 PRIMER_MIN_TM=50 PRIMER_EXPLAIN_FLAG=1 PRIMER_OPT_SIZE=20 PRIMER_NUM_RETURN=1 PRIMER_MAX_SIZE=27 PRIMER_TM_FORMULA=1 SEQUENCE_PRIMER=AGGCTAGGCGAGCTGAAAAATCCTAC SEQUENCE_PRIMER_REVCOMP=GTAGGATTTTTCAGCTCGCCTAGCCT PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_THERMODYNAMIC_PARAMETERS_PATH=./primer3_config/ PRIMER_SALT_CORRECTIONS=1 PRIMER_MAX_HAIRPIN_TH=47 PRIMER_MAX_SELF_ANY_TH=47 PRIMER_MAX_SELF_END_TH=47 PRIMER_PAIR_MAX_COMPL_ANY_TH=30 PRIMER_WARNING=More than one position in template for input oligo AGGCTAGGCGAGCTGAAAAATCCTAC; More than one position in template for input oligo GTAGGATTTTTCAGCTCGCCTAGCCT PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 4, high any compl 3, high end compl 3, left primer to right of right primer 1, ok 3 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=14.595379 PRIMER_LEFT_0_PENALTY=7.297689 PRIMER_RIGHT_0_PENALTY=7.297689 PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high; PRIMER_RIGHT_0_PROBLEMS= Hairpin stability too high; PRIMER_LEFT_0_SEQUENCE=AGGCTAGGCGAGCTGAAAAATCCTAC PRIMER_RIGHT_0_SEQUENCE=GTAGGATTTTTCAGCTCGCCTAGCCT PRIMER_LEFT_0=175,26 PRIMER_RIGHT_0=200,26 PRIMER_LEFT_0_TM=58.702 PRIMER_RIGHT_0_TM=58.702 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY_TH=15.46 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=47.75 PRIMER_RIGHT_0_HAIRPIN_TH=50.05 PRIMER_LEFT_0_END_STABILITY=3.1800 PRIMER_RIGHT_0_END_STABILITY=4.5800 PRIMER_PAIR_0_COMPL_ANY_TH=59.44 PRIMER_PAIR_0_COMPL_END_TH=58.73 PRIMER_PAIR_0_PRODUCT_SIZE=26 = primer3-2.3.6/test/primer3web_v4_0_0_default_settings_input0000664000077300007730000000304612231156245022754 0ustar ioanaioanaSEQUENCE_ID=test_settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag = primer3-2.3.6/test/primer_thermod_align_output0000775000077300007730000032203012231156245020575 0ustar ioanaioanaPRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=0 PRIMER_SALT_DIVALENT=0.0 PRIMER_DNTP_CONC=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 SEQUENCE_ID=thermod_default PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=47.0 PRIMER_MAX_SELF_END_TH=47.0 PRIMER_MAX_HAIRPIN_TH=47.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0 PRIMER_PAIR_MAX_COMPL_END_TH=47.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0 PRIMER_INTERNAL_MAX_SELF_END_TH=47.0 PRIMER_INTERNAL_MAX_HAIRPIN_TH=47.0 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.0 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=47.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_WT_SELF_END_TH=0.0 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0 PRIMER_INTERNAL_WT_SELF_END_TH=0.0 PRIMER_INTERNAL_WT_HAIRPIN_TH=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_END_TH=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=0.0 P3_COMMENT=default values of parameters using thermodynamic approach SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG PRIMER_LEFT_EXPLAIN=considered 1290, GC content failed 10, low tm 60, high tm 1039, ok 181 PRIMER_RIGHT_EXPLAIN=considered 1290, GC content failed 32, low tm 38, high tm 1096, ok 124 PRIMER_INTERNAL_EXPLAIN=considered 1565, GC content failed 32, low tm 60, high tm 1290, ok 183 PRIMER_PAIR_EXPLAIN=considered 25, unacceptable product size 20, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.340791 PRIMER_LEFT_0_PENALTY=0.302821 PRIMER_RIGHT_0_PENALTY=0.037969 PRIMER_INTERNAL_0_PENALTY=0.712692 PRIMER_LEFT_0_SEQUENCE=GACTTCACTTTCCGACCCTG PRIMER_RIGHT_0_SEQUENCE=TGACGGTCATCATGGTGAGT PRIMER_INTERNAL_0_SEQUENCE=CACCCACGAGTGAAGACTCC PRIMER_LEFT_0=11,20 PRIMER_RIGHT_0=113,20 PRIMER_INTERNAL_0=44,20 PRIMER_LEFT_0_TM=59.697 PRIMER_RIGHT_0_TM=59.962 PRIMER_INTERNAL_0_TM=60.713 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=60.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=9.59 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=40.53 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=5.1715 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=3.4242 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=103 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=5.17 PRIMER_PAIR_1_PENALTY=0.340791 PRIMER_LEFT_1_PENALTY=0.302821 PRIMER_RIGHT_1_PENALTY=0.037969 PRIMER_INTERNAL_1_PENALTY=0.712692 PRIMER_LEFT_1_SEQUENCE=TGACTTCACTTTCCGACCCT PRIMER_RIGHT_1_SEQUENCE=TGACGGTCATCATGGTGAGT PRIMER_INTERNAL_1_SEQUENCE=CACCCACGAGTGAAGACTCC PRIMER_LEFT_1=10,20 PRIMER_RIGHT_1=113,20 PRIMER_INTERNAL_1=44,20 PRIMER_LEFT_1_TM=59.697 PRIMER_RIGHT_1_TM=59.962 PRIMER_INTERNAL_1_TM=60.713 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=60.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=9.59 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=40.53 PRIMER_LEFT_1_END_STABILITY=9.1000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=1.7312 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=3.4242 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=104 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=3.42 PRIMER_PAIR_2_PENALTY=0.415766 PRIMER_LEFT_2_PENALTY=0.302821 PRIMER_RIGHT_2_PENALTY=0.112944 PRIMER_INTERNAL_2_PENALTY=0.712692 PRIMER_LEFT_2_SEQUENCE=GACTTCACTTTCCGACCCTG PRIMER_RIGHT_2_SEQUENCE=CTGACGGTCATCATGGTGAG PRIMER_INTERNAL_2_SEQUENCE=CACCCACGAGTGAAGACTCC PRIMER_LEFT_2=11,20 PRIMER_RIGHT_2=114,20 PRIMER_INTERNAL_2=44,20 PRIMER_LEFT_2_TM=59.697 PRIMER_RIGHT_2_TM=60.113 PRIMER_INTERNAL_2_TM=60.713 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=9.19 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=40.53 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=5.1715 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=19.1668 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=104 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=19.17 PRIMER_PAIR_3_PENALTY=0.415766 PRIMER_LEFT_3_PENALTY=0.302821 PRIMER_RIGHT_3_PENALTY=0.112944 PRIMER_INTERNAL_3_PENALTY=0.712692 PRIMER_LEFT_3_SEQUENCE=TGACTTCACTTTCCGACCCT PRIMER_RIGHT_3_SEQUENCE=CTGACGGTCATCATGGTGAG PRIMER_INTERNAL_3_SEQUENCE=CACCCACGAGTGAAGACTCC PRIMER_LEFT_3=10,20 PRIMER_RIGHT_3=114,20 PRIMER_INTERNAL_3=44,20 PRIMER_LEFT_3_TM=59.697 PRIMER_RIGHT_3_TM=60.113 PRIMER_INTERNAL_3_TM=60.713 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=9.19 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=40.53 PRIMER_LEFT_3_END_STABILITY=9.1000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=1.7312 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=19.1668 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=105 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=19.17 PRIMER_PAIR_4_PENALTY=0.749760 PRIMER_LEFT_4_PENALTY=0.711791 PRIMER_RIGHT_4_PENALTY=0.037969 PRIMER_INTERNAL_4_PENALTY=0.712692 PRIMER_LEFT_4_SEQUENCE=GCCGAGGATGACTTCACTTT PRIMER_RIGHT_4_SEQUENCE=TGACGGTCATCATGGTGAGT PRIMER_INTERNAL_4_SEQUENCE=CACCCACGAGTGAAGACTCC PRIMER_LEFT_4=2,20 PRIMER_RIGHT_4=113,20 PRIMER_INTERNAL_4=44,20 PRIMER_LEFT_4_TM=59.288 PRIMER_RIGHT_4_TM=59.962 PRIMER_INTERNAL_4_TM=60.713 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=60.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=9.59 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=40.53 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=6.6985 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=3.4242 PRIMER_PAIR_4_COMPL_ANY_TH=8.76 PRIMER_PAIR_4_COMPL_END_TH=2.04 PRIMER_PAIR_4_PRODUCT_SIZE=112 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=10.12 = SEQUENCE_ID=any_1 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=10.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_LEFT_EXPLAIN=considered 3397, low tm 897, high tm 1543, long poly-x seq 22, ok 935 PRIMER_RIGHT_EXPLAIN=considered 3397, low tm 839, high tm 1673, high hairpin stability 1, long poly-x seq 22, ok 862 PRIMER_INTERNAL_EXPLAIN=considered 3672, low tm 953, high tm 1734, high hairpin stability 14, long poly-x seq 22, ok 949 PRIMER_PAIR_EXPLAIN=considered 58, unacceptable product size 51, ok 7 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.030625 PRIMER_LEFT_0_PENALTY=0.011300 PRIMER_RIGHT_0_PENALTY=0.019325 PRIMER_INTERNAL_0_PENALTY=1.584087 PRIMER_LEFT_0_SEQUENCE=CAAGCAATACGACCTGCTGA PRIMER_RIGHT_0_SEQUENCE=ATCGCAAGTGAGGATGAGCT PRIMER_INTERNAL_0_SEQUENCE=CGTGTGCAAAACATTTTCACA PRIMER_LEFT_0=17,20 PRIMER_RIGHT_0=168,20 PRIMER_INTERNAL_0=116,21 PRIMER_LEFT_0_TM=60.011 PRIMER_RIGHT_0_TM=59.981 PRIMER_INTERNAL_0_TM=60.584 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=38.095 PRIMER_INTERNAL_0_SELF_ANY_TH=12.25 PRIMER_LEFT_0_SELF_ANY_TH=2.42 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=10.49 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=42.83 PRIMER_RIGHT_0_HAIRPIN_TH=39.48 PRIMER_INTERNAL_0_HAIRPIN_TH=37.82 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.4845 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=21.4669 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=152 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=21.47 PRIMER_PAIR_1_PENALTY=0.074511 PRIMER_LEFT_1_PENALTY=0.011300 PRIMER_RIGHT_1_PENALTY=0.063211 PRIMER_INTERNAL_1_PENALTY=0.019325 PRIMER_LEFT_1_SEQUENCE=CAAGCAATACGACCTGCTGA PRIMER_RIGHT_1_SEQUENCE=CAAAAGATTTTGGCGGGTAA PRIMER_INTERNAL_1_SEQUENCE=AGCTCATCCTCACTTGCGAT PRIMER_LEFT_1=17,20 PRIMER_RIGHT_1=191,20 PRIMER_INTERNAL_1=149,20 PRIMER_LEFT_1_TM=60.011 PRIMER_RIGHT_1_TM=59.937 PRIMER_INTERNAL_1_TM=59.981 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=2.42 PRIMER_RIGHT_1_SELF_ANY_TH=5.65 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=42.83 PRIMER_RIGHT_1_HAIRPIN_TH=39.80 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.2000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.4845 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=0.9269 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=175 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=10.41 PRIMER_PAIR_2_PENALTY=0.258931 PRIMER_LEFT_2_PENALTY=0.011300 PRIMER_RIGHT_2_PENALTY=0.247631 PRIMER_INTERNAL_2_PENALTY=0.019325 PRIMER_LEFT_2_SEQUENCE=CAAGCAATACGACCTGCTGA PRIMER_RIGHT_2_SEQUENCE=CCGTACGCACTATAGGAGCC PRIMER_INTERNAL_2_SEQUENCE=AGCTCATCCTCACTTGCGAT PRIMER_LEFT_2=17,20 PRIMER_RIGHT_2=368,20 PRIMER_INTERNAL_2=149,20 PRIMER_LEFT_2_TM=60.011 PRIMER_RIGHT_2_TM=59.752 PRIMER_INTERNAL_2_TM=59.981 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=2.42 PRIMER_RIGHT_2_SELF_ANY_TH=8.58 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=42.83 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=9.4845 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=17.2899 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=352 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=25.59 PRIMER_PAIR_3_PENALTY=0.266957 PRIMER_LEFT_3_PENALTY=0.019325 PRIMER_RIGHT_3_PENALTY=0.247631 PRIMER_INTERNAL_3_PENALTY=0.063211 PRIMER_LEFT_3_SEQUENCE=AGCTCATCCTCACTTGCGAT PRIMER_RIGHT_3_SEQUENCE=CCGTACGCACTATAGGAGCC PRIMER_INTERNAL_3_SEQUENCE=TTACCCGCCAAAATCTTTTG PRIMER_LEFT_3=149,20 PRIMER_RIGHT_3=368,20 PRIMER_INTERNAL_3=172,20 PRIMER_LEFT_3_TM=59.981 PRIMER_RIGHT_3_TM=59.752 PRIMER_INTERNAL_3_TM=59.937 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_SELF_ANY_TH=4.21 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=8.58 PRIMER_INTERNAL_3_SELF_END_TH=4.21 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=43.62 PRIMER_LEFT_3_END_STABILITY=9.8000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=9.8864 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=17.2899 PRIMER_PAIR_3_COMPL_ANY_TH=7.44 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=220 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=27.18 PRIMER_PAIR_4_PENALTY=0.309424 PRIMER_LEFT_4_PENALTY=0.011300 PRIMER_RIGHT_4_PENALTY=0.298125 PRIMER_INTERNAL_4_PENALTY=0.019325 PRIMER_LEFT_4_SEQUENCE=CAAGCAATACGACCTGCTGA PRIMER_RIGHT_4_SEQUENCE=CACTAAAGCGGGATTTGAGG PRIMER_INTERNAL_4_SEQUENCE=AGCTCATCCTCACTTGCGAT PRIMER_LEFT_4=17,20 PRIMER_RIGHT_4=232,20 PRIMER_INTERNAL_4=149,20 PRIMER_LEFT_4_TM=60.011 PRIMER_RIGHT_4_TM=59.702 PRIMER_INTERNAL_4_TM=59.981 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=2.42 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=42.83 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=9.4845 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=2.8328 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=216 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=12.32 = SEQUENCE_ID=any_salt PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=2.5 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=60.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3397, low tm 52, high tm 3139, high hairpin stability 1, long poly-x seq 22, ok 183 PRIMER_RIGHT_EXPLAIN=considered 3397, low tm 59, high tm 3137, long poly-x seq 22, ok 179 PRIMER_INTERNAL_EXPLAIN=considered 3672, low tm 432, high tm 2545, high hairpin stability 10, long poly-x seq 22, ok 663 PRIMER_PAIR_EXPLAIN=considered 15, unacceptable product size 10, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.177993 PRIMER_LEFT_0_PENALTY=0.842396 PRIMER_RIGHT_0_PENALTY=0.335596 PRIMER_INTERNAL_0_PENALTY=0.132562 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.227701 PRIMER_LEFT_1_PENALTY=0.842396 PRIMER_RIGHT_1_PENALTY=0.385305 PRIMER_INTERNAL_1_PENALTY=0.132562 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.418732 PRIMER_LEFT_2_PENALTY=0.385305 PRIMER_RIGHT_2_PENALTY=1.033427 PRIMER_INTERNAL_2_PENALTY=0.172991 PRIMER_LEFT_2_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_2_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_2_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_2=224,20 PRIMER_RIGHT_2=401,20 PRIMER_INTERNAL_2=340,20 PRIMER_LEFT_2_TM=60.385 PRIMER_RIGHT_2_TM=61.033 PRIMER_INTERNAL_2_TM=59.827 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=178 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_3_PENALTY=1.422920 PRIMER_LEFT_3_PENALTY=1.087323 PRIMER_RIGHT_3_PENALTY=0.335596 PRIMER_INTERNAL_3_PENALTY=0.132562 PRIMER_LEFT_3_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_3_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_3_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_3=99,20 PRIMER_RIGHT_3=254,20 PRIMER_INTERNAL_3=145,20 PRIMER_LEFT_3_TM=61.087 PRIMER_RIGHT_3_TM=59.664 PRIMER_INTERNAL_3_TM=59.867 PRIMER_LEFT_3_GC_PERCENT=30.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=5.1000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_4_PENALTY=1.437628 PRIMER_LEFT_4_PENALTY=1.102032 PRIMER_RIGHT_4_PENALTY=0.335596 PRIMER_INTERNAL_4_PENALTY=0.132562 PRIMER_LEFT_4_SEQUENCE=TCTATCTGTACAACGTGTG PRIMER_RIGHT_4_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=103,19 PRIMER_RIGHT_4=254,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=59.898 PRIMER_RIGHT_4_TM=59.664 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=42.105 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=2.59 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.51 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=5.1000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=1.1384 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=152 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=6.60 = SEQUENCE_ID=any_weight PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=2.5 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=47.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0 PRIMER_WT_SELF_ANY_TH=1.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=0.1 PRIMER_PAIR_WT_COMPL_ANY_TH=0.7 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3397, low tm 52, high tm 3139, high hairpin stability 1, long poly-x seq 22, ok 183 PRIMER_RIGHT_EXPLAIN=considered 3397, low tm 59, high tm 3137, long poly-x seq 22, ok 179 PRIMER_INTERNAL_EXPLAIN=considered 3672, low tm 432, high tm 2545, high hairpin stability 10, long poly-x seq 22, ok 663 PRIMER_PAIR_EXPLAIN=considered 15, unacceptable product size 10, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.226125 PRIMER_LEFT_0_PENALTY=0.859989 PRIMER_RIGHT_0_PENALTY=0.353561 PRIMER_INTERNAL_0_PENALTY=0.134205 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.275443 PRIMER_LEFT_1_PENALTY=0.859989 PRIMER_RIGHT_1_PENALTY=0.403040 PRIMER_INTERNAL_1_PENALTY=0.134205 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.466415 PRIMER_LEFT_2_PENALTY=0.403040 PRIMER_RIGHT_2_PENALTY=1.050960 PRIMER_INTERNAL_2_PENALTY=0.174635 PRIMER_LEFT_2_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_2_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_2_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_2=224,20 PRIMER_RIGHT_2=401,20 PRIMER_INTERNAL_2=340,20 PRIMER_LEFT_2_TM=60.385 PRIMER_RIGHT_2_TM=61.033 PRIMER_INTERNAL_2_TM=59.827 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=178 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_3_PENALTY=1.470977 PRIMER_LEFT_3_PENALTY=1.104840 PRIMER_RIGHT_3_PENALTY=0.353561 PRIMER_INTERNAL_3_PENALTY=0.134205 PRIMER_LEFT_3_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_3_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_3_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_3=99,20 PRIMER_RIGHT_3=254,20 PRIMER_INTERNAL_3=145,20 PRIMER_LEFT_3_TM=61.087 PRIMER_RIGHT_3_TM=59.664 PRIMER_INTERNAL_3_TM=59.867 PRIMER_LEFT_3_GC_PERCENT=30.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=5.1000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_4_PENALTY=1.486926 PRIMER_LEFT_4_PENALTY=1.120790 PRIMER_RIGHT_4_PENALTY=0.353561 PRIMER_INTERNAL_4_PENALTY=0.134205 PRIMER_LEFT_4_SEQUENCE=TCTATCTGTACAACGTGTG PRIMER_RIGHT_4_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=103,19 PRIMER_RIGHT_4=254,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=59.898 PRIMER_RIGHT_4_TM=59.664 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=42.105 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=2.59 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.51 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=5.1000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=1.1384 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=152 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=6.60 = SEQUENCE_ID=any_2 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=2.5 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=5.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=5.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=5.0 PRIMER_WT_SELF_ANY_TH=1.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=1.0 PRIMER_PAIR_WT_COMPL_ANY_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3155, low tm 52, high tm 2924, high any compl 32, high hairpin stability 1, long poly-x seq 22, ok 124 PRIMER_RIGHT_EXPLAIN=considered 3213, low tm 59, high tm 2968, high any compl 23, long poly-x seq 19, ok 144 PRIMER_INTERNAL_EXPLAIN=considered 3185, low tm 432, high tm 2188, high any compl 82, high hairpin stability 10, long poly-x seq 22, ok 451 PRIMER_PAIR_EXPLAIN=considered 15, unacceptable product size 10, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.231515 PRIMER_LEFT_0_PENALTY=0.859989 PRIMER_RIGHT_0_PENALTY=0.353561 PRIMER_INTERNAL_0_PENALTY=0.148991 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.280764 PRIMER_LEFT_1_PENALTY=0.859989 PRIMER_RIGHT_1_PENALTY=0.403040 PRIMER_INTERNAL_1_PENALTY=0.148991 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.471735 PRIMER_LEFT_2_PENALTY=0.403040 PRIMER_RIGHT_2_PENALTY=1.050960 PRIMER_INTERNAL_2_PENALTY=0.189431 PRIMER_LEFT_2_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_2_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_2_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_2=224,20 PRIMER_RIGHT_2=401,20 PRIMER_INTERNAL_2=340,20 PRIMER_LEFT_2_TM=60.385 PRIMER_RIGHT_2_TM=61.033 PRIMER_INTERNAL_2_TM=59.827 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=178 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_3_PENALTY=1.476366 PRIMER_LEFT_3_PENALTY=1.104840 PRIMER_RIGHT_3_PENALTY=0.353561 PRIMER_INTERNAL_3_PENALTY=0.148991 PRIMER_LEFT_3_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_3_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_3_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_3=99,20 PRIMER_RIGHT_3=254,20 PRIMER_INTERNAL_3=145,20 PRIMER_LEFT_3_TM=61.087 PRIMER_RIGHT_3_TM=59.664 PRIMER_INTERNAL_3_TM=59.867 PRIMER_LEFT_3_GC_PERCENT=30.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=5.1000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_4_PENALTY=1.492316 PRIMER_LEFT_4_PENALTY=1.120790 PRIMER_RIGHT_4_PENALTY=0.353561 PRIMER_INTERNAL_4_PENALTY=0.148991 PRIMER_LEFT_4_SEQUENCE=TCTATCTGTACAACGTGTG PRIMER_RIGHT_4_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=103,19 PRIMER_RIGHT_4=254,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=59.898 PRIMER_RIGHT_4_TM=59.664 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=42.105 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=2.59 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.51 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=5.1000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=1.1384 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=152 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=6.60 = SEQUENCE_ID=any_no_effect PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=2.5 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=0.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=0.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=0.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3397, low tm 52, high tm 3139, high any compl 1, high hairpin stability 1, long poly-x seq 22, ok 182 PRIMER_RIGHT_EXPLAIN=considered 3397, low tm 59, high tm 3137, long poly-x seq 22, ok 179 PRIMER_INTERNAL_EXPLAIN=considered 3672, low tm 432, high tm 2545, high any compl 11, high hairpin stability 4, long poly-x seq 22, ok 658 PRIMER_PAIR_EXPLAIN=considered 17, unacceptable product size 11, ok 6 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.177993 PRIMER_LEFT_0_PENALTY=0.842396 PRIMER_RIGHT_0_PENALTY=0.335596 PRIMER_INTERNAL_0_PENALTY=0.132562 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.227701 PRIMER_LEFT_1_PENALTY=0.842396 PRIMER_RIGHT_1_PENALTY=0.385305 PRIMER_INTERNAL_1_PENALTY=0.132562 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.418732 PRIMER_LEFT_2_PENALTY=0.385305 PRIMER_RIGHT_2_PENALTY=1.033427 PRIMER_INTERNAL_2_PENALTY=0.172991 PRIMER_LEFT_2_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_2_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_2_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_2=224,20 PRIMER_RIGHT_2=401,20 PRIMER_INTERNAL_2=340,20 PRIMER_LEFT_2_TM=60.385 PRIMER_RIGHT_2_TM=61.033 PRIMER_INTERNAL_2_TM=59.827 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=178 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_3_PENALTY=1.422920 PRIMER_LEFT_3_PENALTY=1.087323 PRIMER_RIGHT_3_PENALTY=0.335596 PRIMER_INTERNAL_3_PENALTY=0.132562 PRIMER_LEFT_3_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_3_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_3_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_3=99,20 PRIMER_RIGHT_3=254,20 PRIMER_INTERNAL_3=145,20 PRIMER_LEFT_3_TM=61.087 PRIMER_RIGHT_3_TM=59.664 PRIMER_INTERNAL_3_TM=59.867 PRIMER_LEFT_3_GC_PERCENT=30.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=5.1000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_4_PENALTY=1.472628 PRIMER_LEFT_4_PENALTY=1.087323 PRIMER_RIGHT_4_PENALTY=0.385305 PRIMER_INTERNAL_4_PENALTY=0.132562 PRIMER_LEFT_4_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_4_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=243,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=61.087 PRIMER_RIGHT_4_TM=60.385 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=30.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=145 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=14.82 = SEQUENCE_ID=end_th1 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_END_TH=10.0 PRIMER_PAIR_MAX_COMPL_END_TH=10.0 PRIMER_INTERNAL_MAX_SELF_END_TH=10.0 PRIMER_WT_SELF_END_TH=0.0 PRIMER_INTERNAL_WT_SELF_END_TH=0.0 PRIMER_PAIR_WT_COMPL_END_TH=0.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3397, low tm 52, high tm 3139, high any compl 1, high hairpin stability 1, long poly-x seq 22, ok 182 PRIMER_RIGHT_EXPLAIN=considered 3397, low tm 59, high tm 3137, long poly-x seq 22, ok 179 PRIMER_INTERNAL_EXPLAIN=considered 3672, low tm 432, high tm 2545, high any compl 11, high hairpin stability 4, long poly-x seq 22, ok 658 PRIMER_PAIR_EXPLAIN=considered 17, unacceptable product size 11, ok 6 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.177993 PRIMER_LEFT_0_PENALTY=0.842396 PRIMER_RIGHT_0_PENALTY=0.335596 PRIMER_INTERNAL_0_PENALTY=0.132562 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.227701 PRIMER_LEFT_1_PENALTY=0.842396 PRIMER_RIGHT_1_PENALTY=0.385305 PRIMER_INTERNAL_1_PENALTY=0.132562 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.418732 PRIMER_LEFT_2_PENALTY=0.385305 PRIMER_RIGHT_2_PENALTY=1.033427 PRIMER_INTERNAL_2_PENALTY=0.172991 PRIMER_LEFT_2_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_2_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_2_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_2=224,20 PRIMER_RIGHT_2=401,20 PRIMER_INTERNAL_2=340,20 PRIMER_LEFT_2_TM=60.385 PRIMER_RIGHT_2_TM=61.033 PRIMER_INTERNAL_2_TM=59.827 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=178 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_3_PENALTY=1.422920 PRIMER_LEFT_3_PENALTY=1.087323 PRIMER_RIGHT_3_PENALTY=0.335596 PRIMER_INTERNAL_3_PENALTY=0.132562 PRIMER_LEFT_3_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_3_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_3_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_3=99,20 PRIMER_RIGHT_3=254,20 PRIMER_INTERNAL_3=145,20 PRIMER_LEFT_3_TM=61.087 PRIMER_RIGHT_3_TM=59.664 PRIMER_INTERNAL_3_TM=59.867 PRIMER_LEFT_3_GC_PERCENT=30.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=5.1000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_4_PENALTY=1.472628 PRIMER_LEFT_4_PENALTY=1.087323 PRIMER_RIGHT_4_PENALTY=0.385305 PRIMER_INTERNAL_4_PENALTY=0.132562 PRIMER_LEFT_4_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_4_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=243,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=61.087 PRIMER_RIGHT_4_TM=60.385 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=30.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=145 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=14.82 = SEQUENCE_ID=end_th2 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_END_TH=47.0 PRIMER_PAIR_MAX_COMPL_END_TH=47.0 PRIMER_INTERNAL_MAX_SELF_END_TH=47.0 PRIMER_WT_SELF_END_TH=0.1 PRIMER_INTERNAL_WT_SELF_END_TH=0.01 PRIMER_PAIR_WT_COMPL_END_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3075, low tm 52, high tm 2860, high any compl 42, long poly-x seq 22, ok 99 PRIMER_RIGHT_EXPLAIN=considered 3118, low tm 59, high tm 2890, high any compl 38, long poly-x seq 17, ok 114 PRIMER_INTERNAL_EXPLAIN=considered 2916, low tm 432, high tm 1999, high any compl 116, high hairpin stability 4, long poly-x seq 22, ok 343 PRIMER_PAIR_EXPLAIN=considered 14, unacceptable product size 5, ok 9 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.199513 PRIMER_LEFT_0_PENALTY=0.844155 PRIMER_RIGHT_0_PENALTY=0.337393 PRIMER_INTERNAL_0_PENALTY=0.132726 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.248969 PRIMER_LEFT_1_PENALTY=0.844155 PRIMER_RIGHT_1_PENALTY=0.387078 PRIMER_INTERNAL_1_PENALTY=0.132726 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.439994 PRIMER_LEFT_2_PENALTY=0.387078 PRIMER_RIGHT_2_PENALTY=1.035180 PRIMER_INTERNAL_2_PENALTY=0.173155 PRIMER_LEFT_2_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_2_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_2_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_2=224,20 PRIMER_RIGHT_2=401,20 PRIMER_INTERNAL_2=340,20 PRIMER_LEFT_2_TM=60.385 PRIMER_RIGHT_2_TM=61.033 PRIMER_INTERNAL_2_TM=59.827 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=178 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_3_PENALTY=1.444433 PRIMER_LEFT_3_PENALTY=1.089075 PRIMER_RIGHT_3_PENALTY=0.337393 PRIMER_INTERNAL_3_PENALTY=0.132726 PRIMER_LEFT_3_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_3_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_3_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_3=99,20 PRIMER_RIGHT_3=254,20 PRIMER_INTERNAL_3=145,20 PRIMER_LEFT_3_TM=61.087 PRIMER_RIGHT_3_TM=59.664 PRIMER_INTERNAL_3_TM=59.867 PRIMER_LEFT_3_GC_PERCENT=30.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=5.1000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_4_PENALTY=1.493888 PRIMER_LEFT_4_PENALTY=1.089075 PRIMER_RIGHT_4_PENALTY=0.387078 PRIMER_INTERNAL_4_PENALTY=0.132726 PRIMER_LEFT_4_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_4_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=243,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=61.087 PRIMER_RIGHT_4_TM=60.385 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=30.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=145 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=14.82 = SEQUENCE_ID=hairpin1 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_HAIRPIN_TH=50.0 PRIMER_INTERNAL_MAX_HAIRPIN_TH=50.0 PRIMER_WT_HAIRPIN_TH=1.0 PRIMER_INTERNAL_WT_HAIRPIN_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3075, low tm 52, high tm 2860, high any compl 42, long poly-x seq 22, ok 99 PRIMER_RIGHT_EXPLAIN=considered 3118, low tm 59, high tm 2890, high any compl 38, long poly-x seq 17, ok 114 PRIMER_INTERNAL_EXPLAIN=considered 2916, low tm 432, high tm 1999, high any compl 116, high hairpin stability 4, long poly-x seq 22, ok 343 PRIMER_PAIR_EXPLAIN=considered 14, unacceptable product size 5, ok 9 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.235070 PRIMER_LEFT_0_PENALTY=0.861748 PRIMER_RIGHT_0_PENALTY=0.355358 PRIMER_INTERNAL_0_PENALTY=0.149156 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.284296 PRIMER_LEFT_1_PENALTY=0.861748 PRIMER_RIGHT_1_PENALTY=0.404813 PRIMER_INTERNAL_1_PENALTY=0.149156 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.475262 PRIMER_LEFT_2_PENALTY=0.404813 PRIMER_RIGHT_2_PENALTY=1.052714 PRIMER_INTERNAL_2_PENALTY=0.189595 PRIMER_LEFT_2_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_2_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_2_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_2=224,20 PRIMER_RIGHT_2=401,20 PRIMER_INTERNAL_2=340,20 PRIMER_LEFT_2_TM=60.385 PRIMER_RIGHT_2_TM=61.033 PRIMER_INTERNAL_2_TM=59.827 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=178 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_3_PENALTY=1.479914 PRIMER_LEFT_3_PENALTY=1.106592 PRIMER_RIGHT_3_PENALTY=0.355358 PRIMER_INTERNAL_3_PENALTY=0.149156 PRIMER_LEFT_3_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_3_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_3_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_3=99,20 PRIMER_RIGHT_3=254,20 PRIMER_INTERNAL_3=145,20 PRIMER_LEFT_3_TM=61.087 PRIMER_RIGHT_3_TM=59.664 PRIMER_INTERNAL_3_TM=59.867 PRIMER_LEFT_3_GC_PERCENT=30.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=5.1000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_4_PENALTY=1.529140 PRIMER_LEFT_4_PENALTY=1.106592 PRIMER_RIGHT_4_PENALTY=0.404813 PRIMER_INTERNAL_4_PENALTY=0.149156 PRIMER_LEFT_4_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_4_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=243,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=61.087 PRIMER_RIGHT_4_TM=60.385 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=30.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=145 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=14.82 = SEQUENCE_ID=hairpin2 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_HAIRPIN_TH=40.0 PRIMER_INTERNAL_MAX_HAIRPIN_TH=40.0 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_INTERNAL_WT_HAIRPIN_TH=0.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3075, low tm 52, high tm 2860, high any compl 42, long poly-x seq 22, ok 99 PRIMER_RIGHT_EXPLAIN=considered 3118, low tm 59, high tm 2890, high any compl 38, long poly-x seq 17, ok 114 PRIMER_INTERNAL_EXPLAIN=considered 2916, low tm 432, high tm 1999, high any compl 116, high hairpin stability 4, long poly-x seq 22, ok 343 PRIMER_PAIR_EXPLAIN=considered 14, unacceptable product size 5, ok 9 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.199513 PRIMER_LEFT_0_PENALTY=0.844155 PRIMER_RIGHT_0_PENALTY=0.337393 PRIMER_INTERNAL_0_PENALTY=0.132726 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.248969 PRIMER_LEFT_1_PENALTY=0.844155 PRIMER_RIGHT_1_PENALTY=0.387078 PRIMER_INTERNAL_1_PENALTY=0.132726 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.439994 PRIMER_LEFT_2_PENALTY=0.387078 PRIMER_RIGHT_2_PENALTY=1.035180 PRIMER_INTERNAL_2_PENALTY=0.173155 PRIMER_LEFT_2_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_2_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_2_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_2=224,20 PRIMER_RIGHT_2=401,20 PRIMER_INTERNAL_2=340,20 PRIMER_LEFT_2_TM=60.385 PRIMER_RIGHT_2_TM=61.033 PRIMER_INTERNAL_2_TM=59.827 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=178 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_3_PENALTY=1.444433 PRIMER_LEFT_3_PENALTY=1.089075 PRIMER_RIGHT_3_PENALTY=0.337393 PRIMER_INTERNAL_3_PENALTY=0.132726 PRIMER_LEFT_3_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_3_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_3_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_3=99,20 PRIMER_RIGHT_3=254,20 PRIMER_INTERNAL_3=145,20 PRIMER_LEFT_3_TM=61.087 PRIMER_RIGHT_3_TM=59.664 PRIMER_INTERNAL_3_TM=59.867 PRIMER_LEFT_3_GC_PERCENT=30.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=5.1000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_4_PENALTY=1.493888 PRIMER_LEFT_4_PENALTY=1.089075 PRIMER_RIGHT_4_PENALTY=0.387078 PRIMER_INTERNAL_4_PENALTY=0.132726 PRIMER_LEFT_4_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_4_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=243,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=61.087 PRIMER_RIGHT_4_TM=60.385 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=30.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=145 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=14.82 = SEQUENCE_ID=hairpin3 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_HAIRPIN_TH=0.0 PRIMER_INTERNAL_MAX_HAIRPIN_TH=47.0 PRIMER_PICK_ANYWAY=1 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_INTERNAL_WT_HAIRPIN_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3075, low tm 52, high tm 2860, high any compl 42, long poly-x seq 22, ok 99 PRIMER_RIGHT_EXPLAIN=considered 3118, low tm 59, high tm 2890, high any compl 38, long poly-x seq 17, ok 114 PRIMER_INTERNAL_EXPLAIN=considered 2916, low tm 432, high tm 1999, high any compl 116, high hairpin stability 22, long poly-x seq 22, ok 325 PRIMER_PAIR_EXPLAIN=considered 14, unacceptable product size 5, ok 9 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.199513 PRIMER_LEFT_0_PENALTY=0.844155 PRIMER_RIGHT_0_PENALTY=0.337393 PRIMER_INTERNAL_0_PENALTY=0.149156 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.248969 PRIMER_LEFT_1_PENALTY=0.844155 PRIMER_RIGHT_1_PENALTY=0.387078 PRIMER_INTERNAL_1_PENALTY=0.149156 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.439994 PRIMER_LEFT_2_PENALTY=0.387078 PRIMER_RIGHT_2_PENALTY=1.035180 PRIMER_INTERNAL_2_PENALTY=0.189595 PRIMER_LEFT_2_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_2_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_2_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_2=224,20 PRIMER_RIGHT_2=401,20 PRIMER_INTERNAL_2=340,20 PRIMER_LEFT_2_TM=60.385 PRIMER_RIGHT_2_TM=61.033 PRIMER_INTERNAL_2_TM=59.827 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.9000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=178 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_3_PENALTY=1.444433 PRIMER_LEFT_3_PENALTY=1.089075 PRIMER_RIGHT_3_PENALTY=0.337393 PRIMER_INTERNAL_3_PENALTY=0.149156 PRIMER_LEFT_3_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_3_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_3_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_3=99,20 PRIMER_RIGHT_3=254,20 PRIMER_INTERNAL_3=145,20 PRIMER_LEFT_3_TM=61.087 PRIMER_RIGHT_3_TM=59.664 PRIMER_INTERNAL_3_TM=59.867 PRIMER_LEFT_3_GC_PERCENT=30.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=5.1000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_4_PENALTY=1.493888 PRIMER_LEFT_4_PENALTY=1.089075 PRIMER_RIGHT_4_PENALTY=0.387078 PRIMER_INTERNAL_4_PENALTY=0.149156 PRIMER_LEFT_4_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_4_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=243,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=61.087 PRIMER_RIGHT_4_TM=60.385 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=30.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=145 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=14.82 = SEQUENCE_ID=template1 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=45.0 PRIMER_WT_TEMPLATE_MISPRIMING_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3075, low tm 52, high tm 2860, high any compl 42, long poly-x seq 22, ok 99 PRIMER_RIGHT_EXPLAIN=considered 3118, low tm 59, high tm 2890, high any compl 38, long poly-x seq 17, ok 114 PRIMER_INTERNAL_EXPLAIN=considered 2916, low tm 432, high tm 1999, high any compl 116, high hairpin stability 22, long poly-x seq 22, ok 325 PRIMER_PAIR_EXPLAIN=considered 14, unacceptable product size 5, ok 9 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.240942 PRIMER_LEFT_0_PENALTY=0.865203 PRIMER_RIGHT_0_PENALTY=0.357774 PRIMER_INTERNAL_0_PENALTY=0.149156 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.294074 PRIMER_LEFT_1_PENALTY=0.865203 PRIMER_RIGHT_1_PENALTY=0.411136 PRIMER_INTERNAL_1_PENALTY=0.149156 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.485215 PRIMER_LEFT_2_PENALTY=1.109476 PRIMER_RIGHT_2_PENALTY=0.357774 PRIMER_INTERNAL_2_PENALTY=0.149156 PRIMER_LEFT_2_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_2_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_2_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_2=99,20 PRIMER_RIGHT_2=254,20 PRIMER_INTERNAL_2=145,20 PRIMER_LEFT_2_TM=61.087 PRIMER_RIGHT_2_TM=59.664 PRIMER_INTERNAL_2_TM=59.867 PRIMER_LEFT_2_GC_PERCENT=30.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=5.1000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=156 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_3_PENALTY=1.492011 PRIMER_LEFT_3_PENALTY=0.412934 PRIMER_RIGHT_3_PENALTY=1.061341 PRIMER_INTERNAL_3_PENALTY=0.189595 PRIMER_LEFT_3_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_3_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_3_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_3=224,20 PRIMER_RIGHT_3=401,20 PRIMER_INTERNAL_3=340,20 PRIMER_LEFT_3_TM=60.385 PRIMER_RIGHT_3_TM=61.033 PRIMER_INTERNAL_3_TM=59.827 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=9.9000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=178 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_4_PENALTY=1.538347 PRIMER_LEFT_4_PENALTY=1.109476 PRIMER_RIGHT_4_PENALTY=0.411136 PRIMER_INTERNAL_4_PENALTY=0.149156 PRIMER_LEFT_4_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_4_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=243,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=61.087 PRIMER_RIGHT_4_TM=60.385 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=30.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=145 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=14.82 = SEQUENCE_ID=template2 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=50.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3075, low tm 52, high tm 2860, high any compl 42, long poly-x seq 22, ok 99 PRIMER_RIGHT_EXPLAIN=considered 3118, low tm 59, high tm 2890, high any compl 38, long poly-x seq 17, ok 114 PRIMER_INTERNAL_EXPLAIN=considered 2916, low tm 432, high tm 1999, high any compl 116, high hairpin stability 22, long poly-x seq 22, ok 325 PRIMER_PAIR_EXPLAIN=considered 14, unacceptable product size 5, ok 9 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.265397 PRIMER_LEFT_0_PENALTY=0.865203 PRIMER_RIGHT_0_PENALTY=0.357774 PRIMER_INTERNAL_0_PENALTY=0.149156 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.318672 PRIMER_LEFT_1_PENALTY=0.865203 PRIMER_RIGHT_1_PENALTY=0.411136 PRIMER_INTERNAL_1_PENALTY=0.149156 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.508939 PRIMER_LEFT_2_PENALTY=1.109476 PRIMER_RIGHT_2_PENALTY=0.357774 PRIMER_INTERNAL_2_PENALTY=0.149156 PRIMER_LEFT_2_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_2_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_2_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_2=99,20 PRIMER_RIGHT_2=254,20 PRIMER_INTERNAL_2=145,20 PRIMER_LEFT_2_TM=61.087 PRIMER_RIGHT_2_TM=59.664 PRIMER_INTERNAL_2_TM=59.867 PRIMER_LEFT_2_GC_PERCENT=30.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=5.1000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=156 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_3_PENALTY=1.542344 PRIMER_LEFT_3_PENALTY=0.412934 PRIMER_RIGHT_3_PENALTY=1.061341 PRIMER_INTERNAL_3_PENALTY=0.189595 PRIMER_LEFT_3_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_3_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_3_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_3=224,20 PRIMER_RIGHT_3=401,20 PRIMER_INTERNAL_3=340,20 PRIMER_LEFT_3_TM=60.385 PRIMER_RIGHT_3_TM=61.033 PRIMER_INTERNAL_3_TM=59.827 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=9.9000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=178 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_4_PENALTY=1.562405 PRIMER_LEFT_4_PENALTY=1.109476 PRIMER_RIGHT_4_PENALTY=0.411136 PRIMER_INTERNAL_4_PENALTY=0.149156 PRIMER_LEFT_4_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_4_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=243,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=61.087 PRIMER_RIGHT_4_TM=60.385 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=30.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=145 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=14.82 = SEQUENCE_ID=template2_and_repeat_library PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_MISPRIMING_LIBRARY=../src/humrep_and_simple.txt PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=50.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3075, low tm 52, high tm 2860, high any compl 42, long poly-x seq 22, ok 99 PRIMER_RIGHT_EXPLAIN=considered 3118, low tm 59, high tm 2890, high any compl 38, long poly-x seq 17, ok 114 PRIMER_INTERNAL_EXPLAIN=considered 2916, low tm 432, high tm 1999, high any compl 116, high hairpin stability 22, long poly-x seq 22, ok 325 PRIMER_PAIR_EXPLAIN=considered 14, unacceptable product size 5, ok 9 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.265397 PRIMER_LEFT_0_PENALTY=0.865203 PRIMER_RIGHT_0_PENALTY=0.357774 PRIMER_INTERNAL_0_PENALTY=0.149156 PRIMER_LEFT_0_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_0_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_0_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=254,20 PRIMER_INTERNAL_0=145,20 PRIMER_LEFT_0_TM=60.842 PRIMER_RIGHT_0_TM=59.664 PRIMER_INTERNAL_0_TM=59.867 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=12.00, MER33 Nonautonomous DNA transposon PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse Tigger2 Autonomous DNA transposon PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, reverse Tigger2 Autonomous DNA transposon PRIMER_LEFT_0_END_STABILITY=8.7000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=14.77 PRIMER_PAIR_1_PENALTY=1.318672 PRIMER_LEFT_1_PENALTY=0.865203 PRIMER_RIGHT_1_PENALTY=0.411136 PRIMER_INTERNAL_1_PENALTY=0.149156 PRIMER_LEFT_1_SEQUENCE=ATTTTTCTATCTGTACAACG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=145,20 PRIMER_LEFT_1_TM=60.842 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.867 PRIMER_LEFT_1_GC_PERCENT=30.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_LIBRARY_MISPRIMING=12.00, MER33 Nonautonomous DNA transposon PRIMER_RIGHT_1_LIBRARY_MISPRIMING=12.00, MER41 Medium reiteration sequence MER41 PRIMER_PAIR_1_LIBRARY_MISPRIMING=19.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_LEFT_1_END_STABILITY=8.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=9.3311 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=15.73 PRIMER_PAIR_2_PENALTY=1.508939 PRIMER_LEFT_2_PENALTY=1.109476 PRIMER_RIGHT_2_PENALTY=0.357774 PRIMER_INTERNAL_2_PENALTY=0.149156 PRIMER_LEFT_2_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_2_SEQUENCE=GACCTGTAATAGCTCACTAC PRIMER_INTERNAL_2_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_2=99,20 PRIMER_RIGHT_2=254,20 PRIMER_INTERNAL_2=145,20 PRIMER_LEFT_2_TM=61.087 PRIMER_RIGHT_2_TM=59.664 PRIMER_INTERNAL_2_TM=59.867 PRIMER_LEFT_2_GC_PERCENT=30.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_LIBRARY_MISPRIMING=11.00, MER33 Nonautonomous DNA transposon PRIMER_RIGHT_2_LIBRARY_MISPRIMING=11.00, reverse Tigger2 Autonomous DNA transposon PRIMER_PAIR_2_LIBRARY_MISPRIMING=19.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=5.1000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=6.5994 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=156 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=13.51 PRIMER_PAIR_3_PENALTY=1.542344 PRIMER_LEFT_3_PENALTY=0.412934 PRIMER_RIGHT_3_PENALTY=1.061341 PRIMER_INTERNAL_3_PENALTY=0.189595 PRIMER_LEFT_3_SEQUENCE=GCTTTAGTGTAGTAGTGAGC PRIMER_RIGHT_3_SEQUENCE=ATACTGATGTCTATAGTGCG PRIMER_INTERNAL_3_SEQUENCE=GCAGAAAAAGGCTCCTATAG PRIMER_LEFT_3=224,20 PRIMER_RIGHT_3=401,20 PRIMER_INTERNAL_3=340,20 PRIMER_LEFT_3_TM=60.385 PRIMER_RIGHT_3_TM=61.033 PRIMER_INTERNAL_3_TM=59.827 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_LIBRARY_MISPRIMING=11.00, MER41 Medium reiteration sequence MER41 PRIMER_RIGHT_3_LIBRARY_MISPRIMING=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence PRIMER_PAIR_3_LIBRARY_MISPRIMING=18.00, MER41 Medium reiteration sequence MER41 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=9.9000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=17.7095 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=18.8085 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=178 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=36.52 PRIMER_PAIR_4_PENALTY=1.562405 PRIMER_LEFT_4_PENALTY=1.109476 PRIMER_RIGHT_4_PENALTY=0.411136 PRIMER_INTERNAL_4_PENALTY=0.149156 PRIMER_LEFT_4_SEQUENCE=TTTTTCTATCTGTACAACGT PRIMER_RIGHT_4_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_4_SEQUENCE=AAACAGCTCATCCTCACTTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=243,20 PRIMER_INTERNAL_4=145,20 PRIMER_LEFT_4_TM=61.087 PRIMER_RIGHT_4_TM=60.385 PRIMER_INTERNAL_4_TM=59.867 PRIMER_LEFT_4_GC_PERCENT=30.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_LIBRARY_MISPRIMING=11.00, MER33 Nonautonomous DNA transposon PRIMER_RIGHT_4_LIBRARY_MISPRIMING=12.00, MER41 Medium reiteration sequence MER41 PRIMER_PAIR_4_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=8.0704 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=14.8192 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=145 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=14.82 = SEQUENCE_ID=template3 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=20 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 3075, low tm 52, high tm 2860, high any compl 42, high hairpin stability 18, high repeat similarity 3, long poly-x seq 22, ok 78 PRIMER_RIGHT_EXPLAIN=considered 3118, low tm 59, high tm 2890, high any compl 38, high hairpin stability 25, long poly-x seq 17, ok 89 PRIMER_INTERNAL_EXPLAIN=considered 2916, low tm 432, high tm 1999, high any compl 116, high hairpin stability 22, long poly-x seq 22, ok 325 PRIMER_PAIR_EXPLAIN=considered 7017, unacceptable product size 2861, high any compl 150, no internal oligo 210, high template mispriming score 97, ok 3699 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1001.568197 PRIMER_LEFT_0_PENALTY=1.163210 PRIMER_RIGHT_0_PENALTY=0.387078 PRIMER_INTERNAL_0_PENALTY=0.057269 PRIMER_LEFT_0_SEQUENCE=CATTCAATACAAGCAATAC PRIMER_RIGHT_0_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_0_SEQUENCE=GCTCATTAAGACCGTACGAT PRIMER_LEFT_0=8,19 PRIMER_RIGHT_0=243,20 PRIMER_INTERNAL_0=72,20 PRIMER_LEFT_0_TM=59.839 PRIMER_RIGHT_0_TM=60.385 PRIMER_INTERNAL_0_TM=59.959 PRIMER_LEFT_0_GC_PERCENT=31.579 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=12.00, TACAA Primate simple repeat pattern PRIMER_RIGHT_0_LIBRARY_MISPRIMING=12.00, MER41 Medium reiteration sequence MER41 PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=5.6000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=5.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=5.0000 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=236 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=10.00 PRIMER_PAIR_1_PENALTY=1002.231568 PRIMER_LEFT_1_PENALTY=1.826755 PRIMER_RIGHT_1_PENALTY=0.387078 PRIMER_INTERNAL_1_PENALTY=0.057269 PRIMER_LEFT_1_SEQUENCE=ATATTCAGTTCATCAAAGG PRIMER_RIGHT_1_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_1_SEQUENCE=GCTCATTAAGACCGTACGAT PRIMER_LEFT_1=46,19 PRIMER_RIGHT_1=243,20 PRIMER_INTERNAL_1=72,20 PRIMER_LEFT_1_TM=60.825 PRIMER_RIGHT_1_TM=60.385 PRIMER_INTERNAL_1_TM=59.959 PRIMER_LEFT_1_GC_PERCENT=31.579 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_GC_PERCENT=45.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_LIBRARY_MISPRIMING=10.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus PRIMER_RIGHT_1_LIBRARY_MISPRIMING=12.00, MER41 Medium reiteration sequence MER41 PRIMER_PAIR_1_LIBRARY_MISPRIMING=19.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING=5.0000 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING=5.0000 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=198 PRIMER_PAIR_1_TEMPLATE_MISPRIMING=10.00 PRIMER_PAIR_2_PENALTY=1002.430683 PRIMER_LEFT_2_PENALTY=1.163210 PRIMER_RIGHT_2_PENALTY=1.249537 PRIMER_INTERNAL_2_PENALTY=0.057269 PRIMER_LEFT_2_SEQUENCE=CATTCAATACAAGCAATAC PRIMER_RIGHT_2_SEQUENCE=TCACTACTACACTAAAGCG PRIMER_INTERNAL_2_SEQUENCE=GCTCATTAAGACCGTACGAT PRIMER_LEFT_2=8,19 PRIMER_RIGHT_2=241,19 PRIMER_INTERNAL_2=72,20 PRIMER_LEFT_2_TM=59.839 PRIMER_RIGHT_2_TM=59.752 PRIMER_INTERNAL_2_TM=59.959 PRIMER_LEFT_2_GC_PERCENT=31.579 PRIMER_RIGHT_2_GC_PERCENT=42.105 PRIMER_INTERNAL_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_LIBRARY_MISPRIMING=12.00, TACAA Primate simple repeat pattern PRIMER_RIGHT_2_LIBRARY_MISPRIMING=11.00, MER41 Medium reiteration sequence MER41 PRIMER_PAIR_2_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=5.6000 PRIMER_RIGHT_2_END_STABILITY=10.2000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING=5.0000 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING=5.0000 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=234 PRIMER_PAIR_2_TEMPLATE_MISPRIMING=10.00 PRIMER_PAIR_3_PENALTY=1002.463065 PRIMER_LEFT_3_PENALTY=2.058252 PRIMER_RIGHT_3_PENALTY=0.387078 PRIMER_INTERNAL_3_PENALTY=0.057269 PRIMER_LEFT_3_SEQUENCE=TCATTCAATACAAGCAATAC PRIMER_RIGHT_3_SEQUENCE=GCTCACTACTACACTAAAGC PRIMER_INTERNAL_3_SEQUENCE=GCTCATTAAGACCGTACGAT PRIMER_LEFT_3=7,20 PRIMER_RIGHT_3=243,20 PRIMER_INTERNAL_3=72,20 PRIMER_LEFT_3_TM=62.057 PRIMER_RIGHT_3_TM=60.385 PRIMER_INTERNAL_3_TM=59.959 PRIMER_LEFT_3_GC_PERCENT=30.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_LIBRARY_MISPRIMING=12.00, TACAA Primate simple repeat pattern PRIMER_RIGHT_3_LIBRARY_MISPRIMING=12.00, MER41 Medium reiteration sequence MER41 PRIMER_PAIR_3_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=5.6000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING=5.0000 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING=5.0000 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=237 PRIMER_PAIR_3_TEMPLATE_MISPRIMING=10.00 PRIMER_PAIR_4_PENALTY=1002.592930 PRIMER_LEFT_4_PENALTY=1.163210 PRIMER_RIGHT_4_PENALTY=1.411811 PRIMER_INTERNAL_4_PENALTY=0.057269 PRIMER_LEFT_4_SEQUENCE=CATTCAATACAAGCAATAC PRIMER_RIGHT_4_SEQUENCE=CTCACTACTACACTAAAGCG PRIMER_INTERNAL_4_SEQUENCE=GCTCATTAAGACCGTACGAT PRIMER_LEFT_4=8,19 PRIMER_RIGHT_4=242,20 PRIMER_INTERNAL_4=72,20 PRIMER_LEFT_4_TM=59.839 PRIMER_RIGHT_4_TM=61.410 PRIMER_INTERNAL_4_TM=59.959 PRIMER_LEFT_4_GC_PERCENT=31.579 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_GC_PERCENT=45.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_LIBRARY_MISPRIMING=12.00, TACAA Primate simple repeat pattern PRIMER_RIGHT_4_LIBRARY_MISPRIMING=12.00, MER41 Medium reiteration sequence MER41 PRIMER_PAIR_4_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=5.6000 PRIMER_RIGHT_4_END_STABILITY=10.2000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING=5.0000 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING=5.0000 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=235 PRIMER_PAIR_4_TEMPLATE_MISPRIMING=10.00 = primer3-2.3.6/test/p3test.pl0000664000077300007730000005622212231156245014623 0ustar ioanaioana # Regression test driver for the primer3_core executable. # # For usage, see the usage statement in the code, below. # # ====================================================================== # (c) Copyright 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2010, # 2011,2012 # Whitehead Institute for Biomedical Research, Steve Rozen, # Andreas Untergasser and Helen Skaletsky # All rights reserved. # # This file is part of the primer3 suite. # # The primer3 suite is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License as # published by the Free Software Foundation; either version 2 of the # License, or (at your option) any later version. # # This software is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # # You should have received a copy of the GNU General Public License # along with this file (file gpl-2.0.txt in the source distribution); if # not, write to the Free Software Foundation, Inc., 51 Franklin St, # Fifth Floor, Boston, MA 02110-1301 USA # # THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS # "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT # LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR # A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT # OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, # SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT # LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, # DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY # THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT # (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE # OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. # ====================================================================== use warnings 'all'; use strict; use Cwd; use Getopt::Long; use POSIX; # For testing wait / system return value, e.g. WIFSIGNALED, WTERMSIG..... use Config; # To get the number for SIGINT sub perldiff($$); sub test_fatal_errors(); sub main(); # Call system() with warnings turned off; needed for ActiveState / MS Windows. sub _nowarn_system($); our $def_executable = "../src/primer3_core"; our $exe = '../src/primer3_core'; our $set_files = '../test/'; our ($verbose, $do_valgrind, $winFlag, $fastFlag, $onetest); our %signo; our $valgrind_format; # Global variable for Errors my $all_ok; main(); sub main() { my %args; # select STDERR; $| = 1; print "$0 for primer3_core version 2.3.6\n"; $all_ok = 1; my $overall_start_time = time(); if (defined $Config{sig_name}) { my $i = 0; for my $name (split(' ', $Config{sig_name})) { $signo{$name} = $i; $i++; } } # GetOptions handles various flag abbreviations and formats, # such as -e ../src/primer3_core, --exe ../src/primer3_core, # --exe=.../src/primer3_core if (!GetOptions(\%args, 'valgrind', 'windows', 'fast', 'verbose', 'onetest=s', 'executable=s', )) { print "Usage: perl p3test.pl \\\n", " [--executable ] [ --onetest ] ", "[ --valgrind ] [ --verbose ] [--windows] [--fast]\n", "\n", " where defaults to ../src/primer3_core\n"; exit -1; } $exe = $args{'executable'} if defined$ args{'executable'}; $winFlag = defined $args{'windows'}; $verbose = defined $args{'verbose'}; $fastFlag = defined $args{'fast'}; $do_valgrind = $args{'valgrind'}; if ($winFlag && $do_valgrind) { print "$0: Cannot specify both --valgrind and --windows\n"; exit -1; } my $valgrind_exe = "/usr/local/bin/valgrind"; my $log_file_arg_for_valgrind = "--log-file-exactly"; if ($do_valgrind) { if (!-x $valgrind_exe) { print "Cannot find $valgrind_exe; will try `which valgrind`\n"; $valgrind_exe= `which valgrind`; chomp($valgrind_exe); if (!$valgrind_exe || ! -x $valgrind_exe) { die "Cannot execute $valgrind_exe"; } } # Need to deal with different arguments in # different version of valgrind my $valgrind_version = `$valgrind_exe --version`; if ($valgrind_version =~ /([0-9]+)\.([0-9]+)\./) { if (($1 > 3) || (($1 == 3) && ($2 > 2))) { $log_file_arg_for_valgrind = "--log-file"; } } } $valgrind_format = $valgrind_exe . " --leak-check=yes --show-reachable=yes " . "$log_file_arg_for_valgrind=%s.vg "; if ($winFlag) { $exe = '..\\src\\primer3_core.exe'; $set_files = '..\\test\\'; } my $exit_stat = 0; die "Cannot execute $exe" unless -x $exe; print "\n\n$0: testing $exe\n\n", "START, ", scalar(localtime), "\n"; print "verbose mode\n" if $verbose; print "valgrind mode\n" if $do_valgrind; # Tests in %default_version2 use --default_version=2. # Others use -default_version=1. my %default_version2 = ('th-w-other-tasks' => 1, 'primer_thermod_align' => 1, 'primer_thermod_align_formatted' => 1, 'primer1_th' => 1, 'primer_mispriming_th' => 1, 'primer_must_use_th' => 1, 'primer_new_tasks_th' => 1, 'primer_task_th' => 1, 'primer_thal_args' => 1, # 'test_compl_error' => 1, # 'test_left_to_right_of_right' => 1, 'primer_boundary' => 1, # Put the quickest tests first. # 'primer_boundary1' => 1, # 'primer_boundary_formatted' => 1, # 'primer_boundary1_formatted' => 1, 'primer3_v1_1_4_default_settings', 'primer3web_v0_4_0_default_settings', 'primer3web_v3_0_0_default_settings', 'primer3web_v4_0_0_default_settings', # 'primer_internal' => 1, # 'primer_internal1' => 1, # 'primer_internal_formatted' => 1, # 'primer_internal1_formatted' => 1, # 'primer_ok_regions' => 1, # 'primer_ok_regions_formatted' => 1, # 'primer_ok_regions2' => 1, # 'primer_tm_lc_masking' => 1, # 'primer_tm_lc_masking_formatted' => 1, # 'primer_start_codon' => 1, # 'primer_task' => 1, # 'primer_task_formatted' => 1, # 'primer_renewed_tasks' => 1, # 'primer_new_tasks' => 1, # 'primer_new_tasks_formatted' => 1, # 'primer_must_overlap_point' => 1, # 'primer_overlap_junction' => 1, # 'primer_all_settingsfiles' => 1, # 'primer_high_tm_load_set' => 1, # 'primer_high_gc_load_set' => 1, # 'primer_gc_end' => 1, # 'primer_check' => 1, # 'primer_must_use' => 1, # 'primer_must_use_formatted' => 1, # 'primer_syntax' => 1, # 'primer_end_pathology' => 1, # 'primer_num_best' => 1, # 'primer_quality_boundary' => 1, # 'primer' => 1, # 'primer1' => 1, # 'primer_mispriming' => 1, # 'primer_mispriming_formatted' => 1, # 'primer_mispriming_boundary1' => 1, # 'primer_mispriming_boundary1_formatted' => 1, # 'primer_mispriming_boundary2' => 1, # 'primer_mispriming_boundary2_formatted' => 1, # 'primer_mispriming_long_lib' => 1, # 'primer_rat' => 1, # 'primer_human' => 1, # 'long_seq' => 1, # 'primer_position_penalty' => 1, # 'primer_position_penalty_formatted' => 1, # 'p3-tmpl-mispriming' => 1, # Put slow tests last # 'p3_3_prime_n' => 1, # 'primer_three_prime_distance' => 1, # 'primer_obj_fn' => 1, # 'primer_lib_amb_codes' => 1, ); my @TESTS = ( # New tests that use new melting temperature # and thermodynamic alignments # Put the quickest tests first. 'primer_must_use_th', 'primer_task_th', 'primer_thal_args', 'primer_thal_max_seq_error', 'primer_first_base_index', 'primer_must_match', 'test_compl_error', 'test_left_to_right_of_right', 'dv_conc_vs_dntp_conc', 'primer_boundary', 'primer_boundary1', 'primer_boundary_formatted', 'primer_boundary1_formatted', 'primer3_v1_1_4_default_settings', 'primer3web_v0_4_0_default_settings', 'primer3web_v3_0_0_default_settings', 'primer3web_v4_0_0_default_settings', 'primer_internal', 'primer_internal1', 'primer_internal_formatted', 'primer_internal1_formatted', 'primer_ok_regions', 'primer_ok_regions_formatted', 'primer_ok_regions2', 'primer_tm_lc_masking', 'primer_tm_lc_masking_formatted', 'primer_start_codon', 'primer_task', 'primer_task_formatted', 'primer_renewed_tasks', 'primer_new_tasks', 'primer_new_tasks_formatted', 'primer_must_overlap_point', 'primer_overlap_junction', 'primer_all_settingsfiles', 'primer_high_tm_load_set', 'primer_high_gc_load_set', 'primer_gc_end', 'primer_check', 'primer_must_use', 'primer_must_use_formatted', 'primer_syntax', 'primer_end_pathology', 'primer_num_best', 'primer_quality_boundary', 'primer', 'primer1', 'primer_mispriming', 'primer_mispriming_formatted', 'primer_mispriming_boundary1', 'primer_mispriming_boundary1_formatted', 'primer_mispriming_boundary2', 'primer_mispriming_boundary2_formatted', 'primer_mispriming_long_lib', 'primer_rat', 'primer_human', 'long_seq', 'primer_position_penalty', 'primer_position_penalty_formatted', 'p3-tmpl-mispriming', 'primer_three_prime_distance', 'primer_obj_fn', 'p3_3_prime_n', # Put slow tests last 'primer_mispriming_th', 'th-w-other-tasks', 'primer_new_tasks_th', 'primer_thermod_align', 'primer_thermod_align_formatted', 'primer1_th', 'primer_lib_amb_codes', ); if (!$args{'onetest'}) { test_fatal_errors(); } else { @TESTS = $args{'onetest'}; } # The range of this for loop is a set of test names # that get translated into file names inside the loop. for my $test (@TESTS) { # We are inside the for loop here.... print "$test..."; my $test_start_time = time(); if ($fastFlag && (($test eq 'th-w-other-tasks') || ($test eq 'primer_obj_fn') || ($test eq 'primer1_th') || ($test eq 'primer_mispriming_th') || ($test eq 'primer_new_tasks_th') || ($test eq 'primer_thermod_align') || ($test eq 'primer_thermod_align_formatted'))) { print "[skiped in fast mode]\n"; next; } my $default_version; if ($default_version2{$test}) { $default_version = '-default_version=2'; } else { $default_version = '-default_version=1'; } if ($test eq 'primer_lib_amb_codes') { if ($fastFlag) { print "[skiped in fast mode]\n"; next; } print "\nNOTE: this test takes _much_ longer than the others ", "(10 to 20 minutes or more).\n", "starting $test at ", scalar(localtime), "..."; } my $valgrind_prefix = $do_valgrind ? sprintf $valgrind_format, $test : ''; # Figure out what the files are called for a particular test my $testx = $test; $testx =~ s/_formatted$//; my $input = $testx . '_input'; my $output = $test . '_output'; my $tmp = $test . '.tmp'; # Make sure that needed files are present and readable die "Cannot read $input" unless -r $input; die "Cannot read $output" unless -r $output; my $r; # Return value for tests my %list_test = ('primer' => 1, 'primer1' => 1, 'primer1_th' => 1, 'th-w-other-tasks' => 1); if (exists($list_test{$test})) { # These tests generate primer lists, which # need to be checked separately. my $list_tmp = $test.'_list_tmp'; # We need to chdir below because primer3 puts the 'list' files # in the current working directory. # get a list of the files to remove (if any) in this directory my @tempList = glob('./' . $test.'_list_tmp/*'); # delete them for my $t (@tempList) { unlink $t; } # go down into primer|primer1 list_tmp directory if (!chdir $list_tmp) { die "chdir $list_tmp: $!\n" } my $tmpCmd; # generate the necc. files; If $winFlag is # set, run command with Windows backslashes # in path. my $settings = ''; if ($winFlag) { if ($test eq 'th-w-other-tasks') { $settings = '-p3_settings ..\\th-w-other-tasks-settings.txt -echo_settings'; } $tmpCmd = "..\\$exe $default_version -strict_tags $settings ../$input >../$tmp"; } else { if ($test eq 'th-w-other-tasks') { $settings = '-p3_settings ../th-w-other-tasks-settings.txt -echo_settings'; } $tmpCmd = "$valgrind_prefix ../$exe $default_version -strict_tags $settings ../$input >../$tmp"; } $r = _nowarn_system($tmpCmd); # back to main directory if (!chdir "../") { die "chdir \"..\": $!\n" } } elsif ($test =~ /settings$/) { my $cmd = "$valgrind_prefix$exe $default_version -strict_tags -p3_settings_file=../$test.txt -echo <$input >$tmp"; $r = _nowarn_system($cmd); } elsif ($test =~ /formatted$/) { my $cmd = "$valgrind_prefix$exe $default_version -strict_tags -format_output <$input >$tmp"; $r = _nowarn_system($cmd); } elsif ($test =~ /_load_set/) { my $cmd = "$valgrind_prefix$exe $default_version -strict_tags -p3_settings_file=$set_files$test.set -echo <$input >$tmp"; $r = _nowarn_system($cmd); } else { my $cmd = "$valgrind_prefix$exe $default_version -strict_tags <$input >$tmp"; $r = _nowarn_system($cmd); } unless ($r == 0) { $all_ok = 0; print "NON-0 EXIT: $r [FAILED]\n"; $exit_stat = -1; next; } $r = perldiff $output, $tmp; print ((time() - $test_start_time), "s "); if ($r == 0) { print "[OK]\n"; } else { $all_ok = 0; print "[FAILED]\n"; $exit_stat = -1; } if (exists($list_test{$test})) { # Special processing for tests that generate files containing # primer lists. my $list_tmp = $test.'_list_tmp'; my $list_last = $test.'_list_last'; if (!-r $list_last) { print "Configuration error, $list_last does not exist or is not readable "; $r = 1; } my @tempList = glob($list_last.'/*'); # do _file by file_ comparison within primer_list_last to primer_list_tmp # since we are not using diff - diff used to do directory comparison for my $t (@tempList) { # sneakily get correct paths since glob of primer_list_last returns # primer_list_last/filename_within_primer_list_last my $regex = "[^/]+/"; $t=~ s/$regex//g; if (perldiff $list_tmp."/".$t, $list_last."/".$t) { $r = 1; } } print $test. "_list_files..."; if ($r == 0) { print "[OK]\n"; } else { $all_ok = 0; print "[FAILED]\n"; $exit_stat = -1; } } } # End of long for loop, for my $test in (.....) # ================================================== # If we were running under valgrind to look for memory-related # errors (reading uninitialized memory, writing off the end of # an array, etc) or leaks, then we look through the valgrind # logs to summarize errors and leaks. if ($do_valgrind) { # Assume this is Unix/Linux envrionment, so # we have grep. my $r = system "grep ERROR *.vg */*.vg | grep -v 'ERROR SUMMARY: 0 errors'"; if (!$r) { # !$r because grep returns 0 if something is found, # and if something is found, we have a problem. $all_ok = 0; print "\nValgrind ERRORs found [FAILED]"; $exit_stat = -1; } $r = system "grep 'definitely lost' *.vg */*.vg | grep -v ' 0 bytes'"; if (!$r) { print "\nValgrind LEAKSs found [WARNING]"; $exit_stat = -1; } $r = system "grep 'possibly lost' *.vg */*.vg | grep -v ' 0 bytes'"; if (!$r) { print "\nValgrind LEAKSs found [WARNING]"; $exit_stat = -1; } } print "\nTests in $0 ran for ", (time() - $overall_start_time), " s \(", ((time() - $overall_start_time)/60), " min\)\n"; print "\nDONE ", scalar(localtime), " "; print $all_ok ? "Passed all tests - [OK]\n\n\n" : "At least one test failed - [FAILED]\n\n\n"; exit 0 # $exit_stat; Change here, generally we want the testing to continue. } # Usage: perldiff("filename1", "filename2") # Return 0 if no differences are found, # othewise return 1; sub perldiff($$) { my ($f1, $f2) = @_; open F1, $f1 or die "open $f1: $!"; open F2, $f2 or die "open $f2: $!"; my @f1 = ; my @f2 = ; # If different number of lines, return FAIL. if (@f1 != @f2) { print "\n Different number of lines;\n"; print " on command line in test directory run:\n"; print " diff $f1 $f2\n\n"; return 1; } # check for differences on the lines, themselves my $linenumber = 0; my $line_end_diff = 0; # iterate using lines in file1 while (@f1) { $linenumber++; # get the lines from each respective file my $l1 = shift @f1; my $l2 = shift @f2; my $l1_orig = $l1; my $l2_orig = $l2; # Handle the diff in empty_1.{out2,tmp2} due to # different executable names. if ($exe ne $def_executable && $l1 =~/^USAGE:\s+\S+/) { $l1 =~ s/^USAGE:\s+\S+/USAGE: ... /i; $l2 =~ s/^USAGE:\s+\S+/USAGE: ... /i; if ($verbose) { print "removing executable name from\n", "$l1_orig\n$l2_orig\n"; } } # Remove everything up to and including the first # colon, which removes the executable name. # This substitution must follow the USAGE # substitution, above my $regex = "[^:]+:"; my $quoteexe = quotemeta($def_executable); # Edit executable name if ($exe ne $def_executable && ($l1 =~ /$quoteexe/ || $l2 =~ /$quoteexe/)) { $l1 =~ s/$regex//g; $l2 =~ s/$regex//g; if ($verbose) { print "removing : from\n", "$l1_orig\n$l2_orig\n"; } } # Edit release number if ($l1 ne $l2) { if ($l1 =~ /(libprimer3|primer3) release \d+\.\d+\.\d+/ && $l2 =~ /(libprimer3|primer3) release \d+\.\d+\.\d+/) { $l1 =~ s/(libprimer3|primer3) release \d+\.\d+\.\d+//; $l2 =~ s/(libprimer3|primer3) release \d+\.\d+\.\d+//; } } # If this is the tag with the settings file path, replace \ by / to make it # the same on both Linux and Windows if ($l1 =~ /^P3_SETTINGS_FILE_USED/ && $l2 =~ /^P3_SETTINGS_FILE_USED/) { $l1 =~ s/\\/\//g; $l2 =~ s/\\/\//g; } $linenumber++; # Check for difference between two edited lines (line by line) if ($l1 ne $l2) { print "\n Difference found at line $linenumber:\n", " < $l1_orig\n", " > $l2_orig\n"; print " There may be additional differences.\n"; print " On command line in test directory run\n\n"; print " diff $f1 $f2\n\n"; return 1; } } return 0; } sub test_fatal_errors() { # Get all the files that match primer_global_err/*.in: my @inputs = glob("./primer_global_err/*.in"); my $r; my $fatal_error_problem = 0; print "\ntesting fatal errors...\n"; my $output_and_err_tested = 0; for (@inputs) { my ($root) = /(.*)\.in$/; # Hint, the parens around $root give # the result of the match in # an array context. print " $root\n"; my $valgrind_prefix = $do_valgrind ? sprintf $valgrind_format, $root : ''; my $cmd; if ($_ =~ /bad_settings\d\.in/) { # For testing the settings files we need the # names of tests and the settings to be parallel $cmd = "$valgrind_prefix$exe -strict_tags -p3_settings_file $_ primer_global_err/input_for_settings_tests.txt > $root.tmp 2> $root.tmp2"; # print STDERR $cmd, "\n"; } else { if ($output_and_err_tested) { $cmd = "$valgrind_prefix$exe -strict_tags <$_ > $root.tmp 2> $root.tmp2"; } else { # We need to test the -output and -error command line arguments plus # simply taking the file name as an argument (no "<") $cmd = "$valgrind_prefix$exe -strict_tags $_ -output $root.tmp -error $root.tmp2"; $output_and_err_tested = 1; print "Testing -output and -error flags on\n$cmd\n"; } } $r = _nowarn_system($cmd); if ($? == 0) { my $r = $? >> 8; print "\nErroneous 0 exit status ($?) from command $cmd\n"; $fatal_error_problem = 1; } if (perldiff "$root.tmp", "$root.out") { print "Difference found between $root.out and $root.tmp\nfrom $cmd\n\n"; $fatal_error_problem = 1; } if (perldiff "$root.tmp2", "$root.out2") { print "\nDifference found between $root.out2 and $root.tmp2\nfrom $cmd\n\n"; $fatal_error_problem = 1; } } if ($fatal_error_problem == 1){ $all_ok = 0; } print(($fatal_error_problem ? "[FAILED]" : "[OK]") ,"\n"); } sub _nowarn_system($) { my $cmd = shift; if ($verbose) { print "\n$cmd\n" } no warnings 'all'; my $r = system $cmd; my $r2 = $?; if (!$winFlag && WIFEXITED($r2)) { $r = WEXITSTATUS($r2); if (defined $signo{'INT'}) { if ($r == $signo{'INT'}) { print "\nCommand: $cmd\n"; print "generated exit value $r\n"; print "Presumably caused by catching SIGINT\n"; print "Tests halted\n\n"; print "\nWARNING: not all tests executed ... [FAILED]\n"; if (!$all_ok) { print "\n\nIn addition, at least 1 test FAILED ... [FAILED]\n\n"; } exit; } } } if (!$winFlag && WIFSIGNALED($r2)) { my $r2 = WTERMSIG($r2); print "Exited with signal $r2\n"; } $r; } primer3-2.3.6/test/test_compl_error_output0000664000077300007730000000140212231156245017757 0ustar ioanaioanaP3_COMMENT=Test error in specifying PRIMER_MAX_SELF_END SEQUENCE_TEMPLATE=CTCTGCCCACATCATGGGTGAGGCCATGGAGCGCATCTATGGTGGACACCTGTGCTACGGTCCGCCCATTGAAAACGGCTTCTACTACGACATGCATCTGGAGGGCGAGGGTATCTCCACCAACGACTACGGCGCCATGGAGGGTCTG SEQUENCE_PRIMER=CTACGACATGCATCTGGAGG PRIMER_MAX_SELF_END=3 PRIMER_EXPLAIN_FLAG=1 PRIMER_TASK=pick_left_only PRIMER_PICK_ANYWAY=1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.733171 PRIMER_LEFT_0_PROBLEMS= Similary to 3' end of self too high; PRIMER_LEFT_0_SEQUENCE=CTACGACATGCATCTGGAGG PRIMER_LEFT_0=84,20 PRIMER_LEFT_0_TM=59.267 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=9.4000 = primer3-2.3.6/test/primer_repeat_boundary1_lib0000664000077300007730000000045512231156245020434 0ustar ioanaioana>test_1 TATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATAT primer3-2.3.6/test/primer1_list_last/0000775000077300007730000000000012231156372016473 5ustar ioanaioanaprimer3-2.3.6/test/primer1_list_last/targets_test.for0000664000077300007730000000065012231156243021711 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TCCTACAGCTGTGGGAAAATG 1 21 0 47.62 60.118 12.00 0.00 1.118 1 CCTACAGCTGTGGGAAAATG 2 20 0 50.00 58.228 10.00 0.00 1.772 2 TCCTACAGCTGTGGGAAAAT 1 20 0 45.00 57.268 12.00 2.00 2.732 primer3-2.3.6/test/primer1_list_last/included_test.rev0000664000077300007730000006241112231156243022040 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 CACAACAGACACACACACACAA 187 22 0 45.45 59.121 1.00 0.00 2.879 1 CAACAGACACACACACACAACA 185 22 0 45.45 59.121 1.00 0.00 2.879 2 CACACACACAACACACAGTCAA 176 22 0 45.45 59.121 3.00 0.00 2.879 3 ACACAACAGACACACACACACAA 188 23 0 43.48 60.064 1.00 0.00 3.064 4 CACAACAGACACACACACACAAC 187 23 0 47.83 60.064 1.00 0.00 3.064 5 ACAACAGACACACACACACAACA 186 23 0 43.48 60.064 1.00 0.00 3.064 6 CAACAGACACACACACACAACAC 185 23 0 47.83 60.064 1.00 0.00 3.064 7 AACAGACACACACACACAACACA 184 23 0 43.48 60.064 1.00 0.00 3.064 8 ACACACACACAACACACAGTCAA 177 23 0 43.48 60.064 3.00 0.00 3.064 9 CACACACACAACACACAGTCAAG 176 23 0 47.83 60.208 3.00 0.00 3.208 10 CACACACAACACACAGTCAAGAA 174 23 0 43.48 59.744 3.00 0.00 3.256 11 AGACACACACACACAACACACAG 181 23 0 47.83 59.650 1.00 1.00 3.350 12 ACACAACAGACACACACACACA 188 22 0 45.45 58.514 1.00 0.00 3.486 13 ACAGACACACACACACAACACA 183 22 0 45.45 58.514 1.00 0.00 3.486 14 CAGACACACACACACAACACAC 182 22 0 50.00 58.514 1.00 0.00 3.486 15 AGACACACACACACAACACACA 181 22 0 45.45 58.514 1.00 0.00 3.486 16 GACACACACACACAACACACAG 180 22 0 50.00 58.514 1.00 1.00 3.486 17 CACACACACACAACACACAGTC 178 22 0 50.00 58.514 3.00 1.00 3.486 18 ACACACACACAACACACAGTCA 177 22 0 45.45 58.514 3.00 1.00 3.486 19 ACAGACACACACACACAACACAC 183 23 0 47.83 59.499 1.00 0.00 3.501 20 GACACACACACACAACACACAGT 180 23 0 47.83 59.499 3.00 3.00 3.501 21 ACACACACACACAACACACAGTC 179 23 0 47.83 59.499 3.00 1.00 3.501 22 CACAACAGACACACACACACA 187 21 0 47.62 57.434 1.00 0.00 3.566 23 CAGACACACACACACAACACA 182 21 0 47.62 57.434 1.00 0.00 3.566 24 CACACACACAACACACAGTCA 176 21 0 47.62 57.434 3.00 1.00 3.566 25 TGCACAATACCACACATGTTT 122 21 0 38.10 57.391 6.00 6.00 3.609 26 GACACACACACACAACACACA 180 21 0 47.62 57.244 1.00 0.00 3.756 27 CACACACAACACACAGTCAAGA 174 22 0 45.45 58.217 3.00 2.00 3.783 28 ACACACACAACACACAGTCAAGA 175 23 0 43.48 59.188 3.00 2.00 3.812 29 CACACAACACACAGTCAAGAAGC 172 23 0 47.83 60.832 3.00 2.00 3.832 30 CACAACACACAGTCAAGAAGCT 170 22 0 45.45 58.075 4.00 4.00 3.925 31 CACAGTCAAGAAGCTACATCCA 163 22 0 45.45 58.036 4.00 0.00 3.964 32 ACACAACACACAGTCAAGAAGCT 171 23 0 43.48 58.981 4.00 4.00 4.019 33 TACACAACAGACACACACACACAA 189 24 0 41.67 60.073 2.00 0.00 4.073 34 CACACAGTCAAGAAGCTACATCC 165 23 0 47.83 58.925 4.00 0.00 4.075 35 ACACAGTCAAGAAGCTACATCCA 164 23 0 43.48 58.925 4.00 0.00 4.075 36 TGCACAATACCACACATGTTTAA 122 23 0 34.78 58.925 6.00 4.00 4.075 37 ACACAACACACAGTCAAGAAGC 171 22 0 45.45 57.918 3.00 2.00 4.082 38 ATGCACAATACCACACATGTTT 123 22 0 36.36 57.811 6.00 6.00 4.189 39 ACACACAGTCAAGAAGCTACATCC 166 24 0 45.83 59.748 4.00 0.00 4.252 40 TGCACAATACCACACATGTTTAAC 122 24 0 37.50 59.733 6.00 5.00 4.267 41 GCACAATACCACACATGTTTAACA 121 24 0 37.50 59.733 7.00 7.00 4.267 42 TACACAACAGACACACACACACA 189 23 0 43.48 58.590 2.00 0.00 4.410 43 TGCACAATACCACACATGTTTA 122 22 0 36.36 57.504 6.00 4.00 4.496 44 GCTACATCCATACACAACACACA 151 23 0 43.48 58.499 2.00 0.00 4.501 45 AGCTACATCCATACACAACACACA 152 24 0 41.67 59.493 4.00 0.00 4.507 46 AGACACACACACACAACACACAGT 181 24 0 45.83 60.539 3.00 3.00 4.539 47 CACAGTCAAGAAGCTACATCCAT 163 23 0 43.48 58.408 4.00 2.00 4.592 48 ACACACACAACACACAGTCAAGAA 175 24 0 41.67 60.599 3.00 0.00 4.599 49 ACACACACACACAACACACAGT 179 22 0 45.45 57.352 3.00 3.00 4.648 50 CACAACACACATGTATATGCACAA 139 24 0 37.50 59.345 6.00 2.00 4.655 51 CAGACACACACACACAACACACA 182 23 0 47.83 61.701 1.00 0.00 4.701 52 CACACACACACAACACACAGTCA 178 23 0 47.83 61.701 3.00 1.00 4.701 53 CACACACAACACACAGTCAAGAAG 174 24 0 45.83 60.730 3.00 0.00 4.730 54 ACACAGTCAAGAAGCTACATCCAT 164 24 0 41.67 59.238 4.00 2.00 4.762 55 ATGCACAATACCACACATGTTTAA 123 24 0 33.33 59.233 6.00 4.00 4.767 56 CACAACACACAGTCAAGAAGCTA 170 23 0 43.48 58.160 4.00 2.00 4.840 57 CAACACACAGTCAAGAAGCTACA 168 23 0 43.48 58.160 4.00 2.00 4.840 58 GAAGCTACATCCATACACAACACA 154 24 0 41.67 59.091 4.00 0.00 4.909 59 ACACACACAACACACAGTCAAG 175 22 0 45.45 57.090 3.00 0.00 4.910 60 ACACAACAGACACACACACACAAC 188 24 0 45.83 60.929 1.00 0.00 4.929 61 ACAACAGACACACACACACAACAC 186 24 0 45.83 60.929 1.00 0.00 4.929 62 AACAGACACACACACACAACACAC 184 24 0 45.83 60.929 1.00 0.00 4.929 63 ACACAACACACAGTCAAGAAGCTA 171 24 0 41.67 59.030 4.00 2.00 4.970 64 CACAACACACAGTCAAGAAGCTAC 170 24 0 45.83 59.030 4.00 4.00 4.970 65 ACAACACACAGTCAAGAAGCTACA 169 24 0 41.67 59.030 4.00 2.00 4.970 66 AGAAGCTACATCCATACACAACACA 155 25 0 40.00 60.002 4.00 0.00 5.002 67 ATGCACAATACCACACATGTTTAAC 123 25 0 36.00 59.991 6.00 5.00 5.009 68 ATACACAACAGACACACACACACA 190 24 0 41.67 58.961 2.00 0.00 5.039 69 ACACACACACAACACACAGTCAAG 177 24 0 45.83 61.059 3.00 0.00 5.059 70 CACAACACACATGTATATGCACA 139 23 0 39.13 57.923 6.00 4.00 5.077 71 TATGCACAATACCACACATGTTT 124 23 0 34.78 57.903 6.00 6.00 5.097 72 ATGCACAATACCACACATGTTTA 123 23 0 34.78 57.903 6.00 4.00 5.097 73 GAAGCTACATCCATACACAACACAC 154 25 0 44.00 59.880 4.00 0.00 5.120 74 ACACAACACACATGTATATGCACAA 140 25 0 36.00 60.151 6.00 2.00 5.151 75 GCTACATCCATACACAACACACAT 151 24 0 41.67 58.842 2.00 2.00 5.158 76 ACACACAACACACAGTCAAGAAG 173 23 0 43.48 57.837 3.00 0.00 5.163 77 ACACAACACACAGTCAAGAAGCTAC 171 25 0 44.00 59.835 4.00 4.00 5.165 78 TCAAGAAGCTACATCCATACACAA 158 24 0 37.50 58.834 4.00 0.00 5.166 79 ACACAACACACATGTATATGCACA 140 24 0 37.50 58.828 6.00 4.00 5.172 80 AGCTACATCCATACACAACACACAT 152 25 0 40.00 59.772 4.00 2.00 5.228 81 CAACACACAGTCAAGAAGCTACATC 168 25 0 44.00 60.272 4.00 2.00 5.272 82 ATACACAACAGACACACACACACAA 190 25 0 40.00 60.344 2.00 0.00 5.344 83 ACATCCATACACAACACACATGT 148 23 0 39.13 57.646 6.00 6.00 5.354 84 CACAACACACATGTATATGCACAAT 139 25 0 36.00 59.637 6.00 2.00 5.363 85 GTCAAGAAGCTACATCCATACACAA 159 25 0 40.00 59.615 4.00 0.00 5.385 86 TCAAGAAGCTACATCCATACACAAC 158 25 0 40.00 59.615 4.00 0.00 5.385 87 CAAGAAGCTACATCCATACACAACA 157 25 0 40.00 60.458 4.00 0.00 5.458 88 CAACACACAGTCAAGAAGCTACAT 168 24 0 41.67 58.520 4.00 4.00 5.480 89 GACACACACACACAACACACAGTC 180 24 0 50.00 61.504 3.00 1.00 5.504 90 CACAGTCAAGAAGCTACATCCATA 163 24 0 41.67 58.476 4.00 2.00 5.524 91 CAGTCAAGAAGCTACATCCATACA 161 24 0 41.67 58.476 4.00 0.00 5.524 92 TCAAGAAGCTACATCCATACACA 158 23 0 39.13 57.467 4.00 0.00 5.533 93 GCACAATACCACACATGTTTAACAG 121 25 0 40.00 60.599 7.00 5.00 5.599 94 ACACACAACACACAGTCAAGAAGC 173 24 0 45.83 61.613 3.00 2.00 5.613 95 CACACAGTCAAGAAGCTACATCCA 165 24 0 45.83 61.619 4.00 0.00 5.619 96 GTCAAGAAGCTACATCCATACACA 159 24 0 41.67 58.339 4.00 0.00 5.661 97 ACAACACACAGTCAAGAAGCTACAT 169 25 0 40.00 59.333 4.00 4.00 5.667 98 CACACATGTATATGCACAATACCA 134 24 0 37.50 58.315 6.00 4.00 5.685 99 CACATGTATATGCACAATACCACA 132 24 0 37.50 58.315 6.00 2.00 5.685 100 CATGTATATGCACAATACCACACA 130 24 0 37.50 58.315 6.00 0.00 5.685 101 TGTATATGCACAATACCACACATG 128 24 0 37.50 58.315 6.00 4.00 5.685 102 ACACAGTCAAGAAGCTACATCCATA 164 25 0 40.00 59.274 4.00 2.00 5.726 103 CACAGTCAAGAAGCTACATCCATAC 163 25 0 44.00 59.274 4.00 0.00 5.726 104 ACAGTCAAGAAGCTACATCCATACA 162 25 0 40.00 59.274 4.00 0.00 5.726 105 CAGTCAAGAAGCTACATCCATACAC 161 25 0 44.00 59.274 4.00 0.00 5.726 106 AGTCAAGAAGCTACATCCATACACA 160 25 0 40.00 59.274 4.00 0.00 5.726 107 CACACAACACACAGTCAAGAAGCT 172 24 0 45.83 61.730 4.00 4.00 5.730 108 TATGCACAATACCACACATGTTTAA 124 25 0 32.00 59.268 6.00 4.00 5.732 109 ATATGCACAATACCACACATGTTT 125 24 0 33.33 58.262 6.00 6.00 5.738 110 AAGCTACATCCATACACAACACACA 153 25 0 40.00 60.742 4.00 0.00 5.742 111 AAGCTACATCCATACACAACACA 153 23 0 39.13 57.257 4.00 0.00 5.743 112 AAGCTACATCCATACACAACACAC 153 24 0 41.67 58.137 4.00 0.00 5.863 113 CCATACACAACACACATGTATATGC 144 25 0 40.00 59.136 6.00 4.00 5.864 114 ACACACATGTATATGCACAATACCA 135 25 0 36.00 59.136 6.00 4.00 5.864 115 CACACATGTATATGCACAATACCAC 134 25 0 40.00 59.136 6.00 2.00 5.864 116 ACACATGTATATGCACAATACCACA 133 25 0 36.00 59.136 6.00 2.00 5.864 117 CACATGTATATGCACAATACCACAC 132 25 0 40.00 59.136 6.00 0.00 5.864 118 ACATGTATATGCACAATACCACACA 131 25 0 36.00 59.136 6.00 0.00 5.864 119 TGTATATGCACAATACCACACATGT 128 25 0 36.00 59.136 6.00 6.00 5.864 120 TACACAACAGACACACACACACAAC 189 25 0 44.00 60.904 2.00 0.00 5.904 121 GCACAATACCACACATGTTTAAC 121 23 0 39.13 57.060 6.00 5.00 5.940 122 AACACACAGTCAAGAAGCTACATCC 167 25 0 44.00 60.962 4.00 0.00 5.962 123 CAAGAAGCTACATCCATACACAA 157 23 0 39.13 57.036 4.00 0.00 5.964 124 TATACACAACAGACACACACACACA 191 25 0 40.00 59.013 4.00 0.00 5.987 125 TATGCACAATACCACACATGTTTAAC 124 26 0 34.62 60.001 6.00 5.00 6.001 126 TATGCACAATACCACACATGTTTA 124 24 0 33.33 57.988 6.00 4.00 6.012 127 ACACAGTCAAGAAGCTACATCCATAC 164 26 0 42.31 60.018 4.00 0.00 6.018 128 ACAGTCAAGAAGCTACATCCATACAC 162 26 0 42.31 60.018 4.00 0.00 6.018 129 CACAATACCACACATGTTTAACAGA 120 25 0 36.00 58.912 7.00 3.00 6.088 130 CAAGAAGCTACATCCATACACAAC 157 24 0 41.67 57.904 4.00 0.00 6.096 131 AAGAAGCTACATCCATACACAACA 156 24 0 37.50 57.904 4.00 0.00 6.096 132 ACACACATGTATATGCACAATACCAC 135 26 0 38.46 59.900 6.00 2.00 6.100 133 ACACATGTATATGCACAATACCACAC 133 26 0 38.46 59.900 6.00 0.00 6.100 134 CTACATCCATACACAACACACATG 150 24 0 41.67 57.896 4.00 4.00 6.104 135 TACACAACACACATGTATATGCACA 141 25 0 36.00 58.882 6.00 4.00 6.118 136 CACACACACAACACACAGTCAAGA 176 24 0 45.83 62.141 3.00 2.00 6.141 137 TACACAACACACATGTATATGCACAA 141 26 0 34.62 60.156 6.00 2.00 6.156 138 CTACATCCATACACAACACACATGT 150 25 0 40.00 58.761 6.00 6.00 6.239 139 TACATCCATACACAACACACATGT 149 24 0 37.50 57.747 6.00 6.00 6.253 140 ACATCCATACACAACACACATGTA 148 24 0 37.50 57.747 6.00 4.00 6.253 141 AAGAAGCTACATCCATACACAACAC 156 25 0 40.00 58.712 4.00 0.00 6.288 142 CACAATACCACACATGTTTAACAGAC 120 26 0 38.46 59.680 7.00 1.00 6.320 143 CACAACACACATGTATATGCACAATA 139 26 0 34.62 59.659 6.00 4.00 6.341 144 TATACACAACAGACACACACACACAA 191 26 0 38.46 60.342 4.00 0.00 6.342 145 GTCAAGAAGCTACATCCATACACAAC 159 26 0 42.31 60.343 4.00 0.00 6.343 146 CAACACACATGTATATGCACAATACC 137 26 0 38.46 60.346 6.00 4.00 6.346 147 AACACACATGTATATGCACAATACCA 136 26 0 34.62 60.346 6.00 4.00 6.346 148 TGTATATGCACAATACCACACATGTT 128 26 0 34.62 60.346 6.00 4.00 6.346 149 CATGTATATGCACAATACCACACAT 130 25 0 36.00 58.650 6.00 2.00 6.350 150 ATGTATATGCACAATACCACACATG 129 25 0 36.00 58.650 6.00 4.00 6.350 151 AACACACAGTCAAGAAGCTACATC 167 24 0 41.67 57.626 4.00 2.00 6.374 152 ACACAACACACATGTATATGCACAAT 140 26 0 34.62 60.393 6.00 2.00 6.393 153 AGTCAAGAAGCTACATCCATACACAA 160 26 0 38.46 60.455 4.00 0.00 6.455 154 ATATGCACAATACCACACATGTTTAA 125 26 0 30.77 59.536 6.00 4.00 6.464 155 GCTACATCCATACACAACACACATG 151 25 0 44.00 61.472 4.00 4.00 6.472 156 ACACACACAACACACAGTCAAGAAG 175 25 0 44.00 61.508 3.00 0.00 6.508 157 ACAGACACACACACACAACACACA 183 24 0 45.83 62.520 1.00 0.00 6.520 158 ACACACACACACAACACACAGTCA 179 24 0 45.83 62.520 3.00 1.00 6.520 159 ACATGTATATGCACAATACCACACAT 131 26 0 34.62 59.420 6.00 2.00 6.580 160 ATGTATATGCACAATACCACACATGT 129 26 0 34.62 59.420 6.00 6.00 6.580 161 AGAAGCTACATCCATACACAACAC 155 24 0 41.67 57.400 4.00 0.00 6.600 162 CATACACAACACACATGTATATGCA 143 25 0 36.00 58.378 6.00 6.00 6.622 163 CAGACACACACACACAACACACAG 182 24 0 50.00 62.641 1.00 1.00 6.641 164 TATATGCACAATACCACACATGTTT 126 25 0 32.00 58.331 6.00 6.00 6.669 165 ATATGCACAATACCACACATGTTTA 125 25 0 32.00 58.331 6.00 4.00 6.669 166 ATATACACAACAGACACACACACACA 192 26 0 38.46 59.329 4.00 0.00 6.671 167 CACACAACACACAGTCAAGAAGCTA 172 25 0 44.00 61.678 4.00 2.00 6.678 168 CACAACACACAGTCAAGAAGCTACA 170 25 0 44.00 61.678 4.00 2.00 6.678 169 AGAAGCTACATCCATACACAACACAC 155 26 0 42.31 60.727 4.00 0.00 6.727 170 CATCCATACACAACACACATGTAT 147 24 0 37.50 57.241 6.00 6.00 6.759 171 CACACACACACAACATACACTCTAGA 242 26 0 42.31 59.211 6.00 6.00 6.789 172 CACACACACAACATACACTCTAGACA 240 26 0 42.31 59.211 6.00 2.00 6.789 173 CACACACAACATACACTCTAGACACA 238 26 0 42.31 59.211 6.00 0.00 6.789 174 CACACAGTCAAGAAGCTACATCCAT 165 25 0 44.00 61.791 4.00 2.00 6.791 175 CATACACAACACACATGTATATGCAC 143 26 0 38.46 59.190 6.00 4.00 6.810 176 ATACACAACACACATGTATATGCACA 142 26 0 34.62 59.190 6.00 4.00 6.810 177 TCCATACACAACACACATGTATATGC 145 26 0 38.46 60.811 6.00 4.00 6.811 178 CACAATACCACACATGTTTAACAG 120 24 0 37.50 57.140 7.00 5.00 6.860 179 ACATCCATACACAACACACATGTAT 148 25 0 36.00 58.124 6.00 6.00 6.876 180 ACACACACACACAACATACACTCT 243 24 0 41.67 57.096 2.00 1.00 6.904 181 GTATATGCACAATACCACACATGTTT 127 26 0 34.62 59.095 6.00 6.00 6.905 182 TCTACACACACACACAACATACACTC 246 26 0 42.31 59.077 2.00 0.00 6.923 183 AAGCTACATCCATACACAACACACAT 153 26 0 38.46 60.948 4.00 2.00 6.948 184 TATATGCACAATACCACACATGTT 126 24 0 33.33 57.002 6.00 4.00 6.998 185 TCTACACACACACACAACATACACTCT 246 27 0 40.74 60.001 2.00 1.00 7.001 186 ACACACACACACAACATACACTCTAGA 243 27 0 40.74 60.001 6.00 6.00 7.001 187 CACACACACACAACATACACTCTAGAC 242 27 0 44.44 60.001 6.00 4.00 7.001 188 ACACACACACAACATACACTCTAGACA 241 27 0 40.74 60.001 6.00 2.00 7.001 189 CACACACACAACATACACTCTAGACAC 240 27 0 44.44 60.001 6.00 0.00 7.001 190 ACACACACAACATACACTCTAGACACA 239 27 0 40.74 60.001 6.00 0.00 7.001 191 ACAACACACAGTCAAGAAGCTACATC 169 26 0 42.31 61.001 4.00 2.00 7.001 192 ATACACAACAGACACACACACACAAC 190 26 0 42.31 61.121 2.00 0.00 7.121 193 CACACACAACATACACTCTAGACACAG 238 27 0 44.44 60.122 6.00 1.00 7.122 194 CATGTATATGCACAATACCACACATG 130 26 0 38.46 61.147 6.00 4.00 7.147 195 AACACACATGTATATGCACAATACC 136 25 0 36.00 57.851 6.00 4.00 7.149 196 GTATATGCACAATACCACACATGTT 127 25 0 36.00 57.851 6.00 4.00 7.149 197 CAAGAAGCTACATCCATACACAACAC 157 26 0 42.31 61.157 4.00 0.00 7.157 198 AAGAAGCTACATCCATACACAACACA 156 26 0 38.46 61.157 4.00 0.00 7.157 199 TACATCCATACACAACACACATGTA 149 25 0 36.00 57.841 6.00 4.00 7.159 200 CTACATCCATACACAACACACATGTA 150 26 0 38.46 58.815 6.00 4.00 7.185 201 ATATGCACAATACCACACATGTTTAAC 125 27 0 33.33 60.227 6.00 5.00 7.227 202 CATCCATACACAACACACATGTATATG 147 27 0 37.04 60.230 6.00 6.00 7.230 203 AACACACGTGTATATACACAACAGACA 203 27 0 37.04 59.748 12.00 1.00 7.252 204 TGCACAATACCACACATGTTTAACA 122 25 0 36.00 62.264 7.00 7.00 7.264 205 ACACACAGTCAAGAAGCTACATCCA 166 25 0 44.00 62.322 4.00 0.00 7.322 206 CACACGTGTATATACACAACAGACA 201 25 0 40.00 57.664 12.00 1.00 7.336 207 CACGTGTATATACACAACAGACACA 199 25 0 40.00 57.664 12.00 1.00 7.336 208 CGTGTATATACACAACAGACACACA 197 25 0 40.00 57.664 12.00 0.00 7.336 209 ATACACAACACACATGTATATGCACAA 142 27 0 33.33 60.389 6.00 2.00 7.389 210 TACACAACACACATGTATATGCACAAT 141 27 0 33.33 60.389 6.00 2.00 7.389 211 ACACAACACACATGTATATGCACAATA 140 27 0 33.33 60.389 6.00 4.00 7.389 212 CACAACACACATGTATATGCACAATAC 139 27 0 37.04 60.389 6.00 6.00 7.389 213 AGACACACACACACAACACACAGTC 181 25 0 48.00 62.414 3.00 1.00 7.414 214 TATATGCACAATACCACACATGTTTAA 126 27 0 29.63 59.560 6.00 4.00 7.440 215 ACACACAACACACAGTCAAGAAGCT 173 25 0 44.00 62.443 4.00 4.00 7.443 216 GATCTACACACACACACAACATACA 248 25 0 40.00 57.548 4.00 0.00 7.452 217 ACACACGTGTATATACACAACAGACA 202 26 0 38.46 58.508 12.00 1.00 7.492 218 CACACGTGTATATACACAACAGACAC 201 26 0 42.31 58.508 12.00 3.00 7.492 219 ACACGTGTATATACACAACAGACACA 200 26 0 38.46 58.508 12.00 1.00 7.492 220 CACGTGTATATACACAACAGACACAC 199 26 0 42.31 58.508 12.00 1.00 7.492 221 ACGTGTATATACACAACAGACACACA 198 26 0 38.46 58.508 12.00 0.00 7.492 222 CGTGTATATACACAACAGACACACAC 197 26 0 42.31 58.508 12.00 0.00 7.492 223 ATGTATATGCACAATACCACACATGTT 129 27 0 33.33 60.562 6.00 4.00 7.562 224 GATCTACACACACACACAACATACAC 248 26 0 42.31 58.431 4.00 0.00 7.569 225 ATATACACAACAGACACACACACACAA 192 27 0 37.04 60.577 4.00 0.00 7.577 226 TATATGCACAATACCACACATGTTTA 126 26 0 30.77 58.395 6.00 4.00 7.605 227 GATCTACACACACACACAACATACACT 248 27 0 40.74 59.376 4.00 1.00 7.624 228 ATCTACACACACACACAACATACACTC 247 27 0 40.74 59.376 2.00 0.00 7.624 229 CACACACACACAACATACACTCTAG 242 25 0 44.00 57.370 4.00 4.00 7.630 230 TGTATATACACAACAGACACACACACA 195 27 0 37.04 59.363 10.00 0.00 7.637 231 TATATACACAACAGACACACACACACA 193 27 0 37.04 59.363 6.00 0.00 7.637 232 CATCCATACACAACACACATGTATA 147 25 0 36.00 57.352 6.00 4.00 7.648 233 TCCATACACAACACACATGTATATG 145 25 0 36.00 57.352 6.00 6.00 7.648 234 CCATACACAACACACATGTATATGCA 144 26 0 38.46 61.654 6.00 6.00 7.654 235 CACACATGTATATGCACAATACCACA 134 26 0 38.46 61.654 6.00 2.00 7.654 236 CACATGTATATGCACAATACCACACA 132 26 0 38.46 61.654 6.00 0.00 7.654 237 ACACACGTGTATATACACAACAGACAC 202 27 0 40.74 59.293 12.00 3.00 7.707 238 ACACGTGTATATACACAACAGACACAC 200 27 0 40.74 59.293 12.00 1.00 7.707 239 ACGTGTATATACACAACAGACACACAC 198 27 0 40.74 59.293 12.00 0.00 7.707 240 CACACAGTCAAGAAGCTACATCCATA 165 26 0 42.31 61.740 4.00 2.00 7.740 241 CACAGTCAAGAAGCTACATCCATACA 163 26 0 42.31 61.740 4.00 0.00 7.740 242 CAGTCAAGAAGCTACATCCATACACA 161 26 0 42.31 61.740 4.00 0.00 7.740 243 CTACACACACACACAACATACACTCT 245 26 0 42.31 58.251 2.00 1.00 7.749 244 ACACACACACACAACATACACTCTAG 243 26 0 42.31 58.251 4.00 4.00 7.749 245 TCTACACACACACACAACATACACT 246 25 0 40.00 57.217 2.00 1.00 7.783 246 CTACACACACACACAACATACACTC 245 25 0 44.00 57.217 2.00 0.00 7.783 247 TACACACACACACAACATACACTCT 244 25 0 40.00 57.217 2.00 1.00 7.783 248 ACACACACACACAACATACACTCTA 243 25 0 40.00 57.217 3.00 3.00 7.783 249 TACATCCATACACAACACACATGTAT 149 26 0 34.62 58.200 6.00 6.00 7.800 250 ACATCCATACACAACACACATGTATA 148 26 0 34.62 58.200 6.00 4.00 7.800 251 CACAACACACAGTCAAGAAGCTACAT 170 26 0 42.31 61.844 4.00 4.00 7.844 252 GAACACACGTGTATATACACAACAGA 204 26 0 38.46 58.139 12.00 4.00 7.861 253 GTATATGCACAATACCACACATGTTTA 127 27 0 33.33 59.133 6.00 4.00 7.867 254 CTACATCCATACACAACACACATGTAT 150 27 0 37.04 59.113 6.00 6.00 7.887 255 ACACACACACAACACACAGTCAAGA 177 25 0 44.00 62.889 3.00 2.00 7.889 256 ACAACACACATGTATATGCACAATA 138 25 0 32.00 57.073 6.00 4.00 7.927 257 CAACACACATGTATATGCACAATAC 137 25 0 36.00 57.073 6.00 6.00 7.927 258 AGAACACACGTGTATATACACAACAGA 205 27 0 37.04 59.049 12.00 4.00 7.951 259 ATCCATACACAACACACATGTATATGC 146 27 0 37.04 61.010 6.00 4.00 8.010 260 ACAACACACATGTATATGCACAATACC 138 27 0 37.04 61.034 6.00 4.00 8.034 261 AACACACATGTATATGCACAATACCAC 136 27 0 37.04 61.034 6.00 2.00 8.034 262 CACACGTGTATATACACAACAGACACA 201 27 0 40.74 61.042 12.00 1.00 8.042 263 CACGTGTATATACACAACAGACACACA 199 27 0 40.74 61.042 12.00 0.00 8.042 264 CGTGTATATACACAACAGACACACACA 197 27 0 40.74 61.042 12.00 0.00 8.042 265 TCAAGAAGCTACATCCATACACAACA 158 26 0 38.46 62.056 4.00 0.00 8.056 266 GAACACACGTGTATATACACAACAGAC 204 27 0 40.74 58.926 12.00 2.00 8.074 267 ACAACACACATGTATATGCACAATAC 138 26 0 34.62 57.913 6.00 6.00 8.087 268 TATACACAACAGACACACACACACAAC 191 27 0 40.74 61.092 4.00 0.00 8.092 269 AGTCAAGAAGCTACATCCATACACAAC 160 27 0 40.74 61.126 4.00 0.00 8.126 270 GCTACATCCATACACAACACACATGT 151 26 0 42.31 62.163 6.00 6.00 8.163 271 GCACAATACCACACATGTTTAACAGA 121 26 0 38.46 62.185 7.00 3.00 8.185 272 AGCTACATCCATACACAACACACATG 152 26 0 42.31 62.266 4.00 4.00 8.266 273 CATCCATACACAACACACATGTATAT 147 26 0 34.62 57.730 6.00 6.00 8.270 274 ATCCATACACAACACACATGTATATG 146 26 0 34.62 57.730 6.00 6.00 8.270 275 ACACACAACACACAGTCAAGAAGCTA 173 26 0 42.31 62.367 4.00 2.00 8.367 276 CACACAACACACAGTCAAGAAGCTAC 172 26 0 46.15 62.367 4.00 4.00 8.367 277 ACACAACACACAGTCAAGAAGCTACA 171 26 0 42.31 62.367 4.00 2.00 8.367 278 GAAGCTACATCCATACACAACACACA 154 26 0 42.31 62.368 4.00 0.00 8.368 279 ATCTACACACACACACAACATACACT 247 26 0 38.46 57.620 2.00 1.00 8.380 280 ATGCACAATACCACACATGTTTAACA 123 26 0 34.62 62.397 7.00 7.00 8.397 281 TGTATATGCACAATACCACACATGTTT 128 27 0 33.33 61.445 6.00 6.00 8.445 282 ACACACAGTCAAGAAGCTACATCCAT 166 26 0 42.31 62.454 4.00 2.00 8.454 283 ACATCCATACACAACACACATGTATAT 148 27 0 33.33 58.526 6.00 6.00 8.474 284 ACACACAACATACACTCTAGACACAG 237 26 0 42.31 57.508 6.00 1.00 8.492 285 AGAACACACGTGTATATACACAACAG 205 26 0 38.46 57.375 12.00 6.00 8.625 286 ACACACACACAACATACACTCTAGAC 241 26 0 42.31 57.364 6.00 4.00 8.636 287 ACACACACAACATACACTCTAGACAC 239 26 0 42.31 57.364 6.00 0.00 8.636 288 TACACACACACACAACATACACTCTA 244 26 0 38.46 57.330 3.00 3.00 8.670 289 CTACACACACACACAACATACACTCTA 245 27 0 40.74 58.323 3.00 3.00 8.677 290 TACACACACACACAACATACACTCTAG 244 27 0 40.74 58.323 4.00 4.00 8.677 291 CATACACAACACACATGTATATGCACA 143 27 0 37.04 61.688 6.00 4.00 8.688 292 TACATCCATACACAACACACATGTATA 149 27 0 33.33 58.271 6.00 4.00 8.729 293 AACACACGTGTATATACACAACAGAC 203 26 0 38.46 57.237 12.00 2.00 8.763 294 AAGAAGCTACATCCATACACAACACAC 156 27 0 40.74 61.811 4.00 0.00 8.811 295 CACATGTATATGCACAATACCACACAT 132 27 0 37.04 61.814 6.00 2.00 8.814 296 ACATGTATATGCACAATACCACACATG 131 27 0 37.04 61.814 6.00 4.00 8.814 297 CATGTATATGCACAATACCACACATGT 130 27 0 37.04 61.814 6.00 6.00 8.814 298 CACAATACCACACATGTTTAACAGACA 120 27 0 37.04 62.089 7.00 3.00 9.089 299 GCTACATCCATACACAACACACATGTA 151 27 0 40.74 62.100 6.00 4.00 9.100 300 CCATACACAACACACATGTATATGCAC 144 27 0 40.74 62.312 6.00 4.00 9.312 301 ACACACATGTATATGCACAATACCACA 135 27 0 37.04 62.312 6.00 2.00 9.312 302 CACACATGTATATGCACAATACCACAC 134 27 0 40.74 62.312 6.00 0.00 9.312 303 ACACATGTATATGCACAATACCACACA 133 27 0 37.04 62.312 6.00 0.00 9.312 304 TATGCACAATACCACACATGTTTAACA 124 27 0 33.33 62.329 7.00 7.00 9.329 305 ACACACAGTCAAGAAGCTACATCCATA 166 27 0 40.74 62.383 4.00 2.00 9.383 306 CACACAGTCAAGAAGCTACATCCATAC 165 27 0 44.44 62.383 4.00 0.00 9.383 307 ACACAGTCAAGAAGCTACATCCATACA 164 27 0 40.74 62.383 4.00 0.00 9.383 308 CACAGTCAAGAAGCTACATCCATACAC 163 27 0 44.44 62.383 4.00 0.00 9.383 309 ACAGTCAAGAAGCTACATCCATACACA 162 27 0 40.74 62.383 4.00 0.00 9.383 310 GTGTATATACACAACAGACACACACAC 196 27 0 40.74 57.575 12.00 0.00 9.425 311 GTATATACACAACAGACACACACACAC 194 27 0 40.74 57.575 8.00 0.00 9.425 312 GAAGCTACATCCATACACAACACACAT 154 27 0 40.74 62.494 4.00 2.00 9.494 313 ACACAACACACAGTCAAGAAGCTACAT 171 27 0 40.74 62.495 4.00 4.00 9.495 314 TAGAACACACGTGTATATACACAACAG 206 27 0 37.04 57.472 12.00 6.00 9.528 315 GTCAAGAAGCTACATCCATACACAACA 159 27 0 40.74 62.685 4.00 0.00 9.685 316 TCAAGAAGCTACATCCATACACAACAC 158 27 0 40.74 62.685 4.00 0.00 9.685 317 CAACACACATGTATATGCACAATACCA 137 27 0 37.04 62.716 6.00 4.00 9.716 318 CAGTCAAGAAGCTACATCCATACACAA 161 27 0 40.74 62.778 4.00 0.00 9.778 319 GCACAATACCACACATGTTTAACAGAC 121 27 0 40.74 62.806 7.00 1.00 9.806 320 AGCTACATCCATACACAACACACATGT 152 27 0 40.74 62.902 6.00 6.00 9.902 primer3-2.3.6/test/primer1_list_last/SP01-21.txt.rev0000664000077300007730000006227112231156243020737 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 CACAACAGACACACACACACAA 167 22 0 45.45 59.121 1.00 0.00 2.879 1 CAACAGACACACACACACAACA 165 22 0 45.45 59.121 1.00 0.00 2.879 2 CACACACACAACACACAGTCAA 156 22 0 45.45 59.121 3.00 0.00 2.879 3 ACACAACAGACACACACACACAA 168 23 0 43.48 60.064 1.00 0.00 3.064 4 CACAACAGACACACACACACAAC 167 23 0 47.83 60.064 1.00 0.00 3.064 5 ACAACAGACACACACACACAACA 166 23 0 43.48 60.064 1.00 0.00 3.064 6 CAACAGACACACACACACAACAC 165 23 0 47.83 60.064 1.00 0.00 3.064 7 AACAGACACACACACACAACACA 164 23 0 43.48 60.064 1.00 0.00 3.064 8 ACACACACACAACACACAGTCAA 157 23 0 43.48 60.064 3.00 0.00 3.064 9 CACACACACAACACACAGTCAAG 156 23 0 47.83 60.208 3.00 0.00 3.208 10 CACACACAACACACAGTCAAGAA 154 23 0 43.48 59.744 3.00 0.00 3.256 11 AGACACACACACACAACACACAG 161 23 0 47.83 59.650 1.00 1.00 3.350 12 ACACAACAGACACACACACACA 168 22 0 45.45 58.514 1.00 0.00 3.486 13 ACAGACACACACACACAACACA 163 22 0 45.45 58.514 1.00 0.00 3.486 14 CAGACACACACACACAACACAC 162 22 0 50.00 58.514 1.00 0.00 3.486 15 AGACACACACACACAACACACA 161 22 0 45.45 58.514 1.00 0.00 3.486 16 GACACACACACACAACACACAG 160 22 0 50.00 58.514 1.00 1.00 3.486 17 CACACACACACAACACACAGTC 158 22 0 50.00 58.514 3.00 1.00 3.486 18 ACACACACACAACACACAGTCA 157 22 0 45.45 58.514 3.00 1.00 3.486 19 ACAGACACACACACACAACACAC 163 23 0 47.83 59.499 1.00 0.00 3.501 20 GACACACACACACAACACACAGT 160 23 0 47.83 59.499 3.00 3.00 3.501 21 ACACACACACACAACACACAGTC 159 23 0 47.83 59.499 3.00 1.00 3.501 22 CACAACAGACACACACACACA 167 21 0 47.62 57.434 1.00 0.00 3.566 23 CAGACACACACACACAACACA 162 21 0 47.62 57.434 1.00 0.00 3.566 24 CACACACACAACACACAGTCA 156 21 0 47.62 57.434 3.00 1.00 3.566 25 TGCACAATACCACACATGTTT 102 21 0 38.10 57.391 6.00 6.00 3.609 26 GACACACACACACAACACACA 160 21 0 47.62 57.244 1.00 0.00 3.756 27 CACACACAACACACAGTCAAGA 154 22 0 45.45 58.217 3.00 2.00 3.783 28 ACACACACAACACACAGTCAAGA 155 23 0 43.48 59.188 3.00 2.00 3.812 29 CACACAACACACAGTCAAGAAGC 152 23 0 47.83 60.832 3.00 2.00 3.832 30 CACAACACACAGTCAAGAAGCT 150 22 0 45.45 58.075 4.00 4.00 3.925 31 CACAGTCAAGAAGCTACATCCA 143 22 0 45.45 58.036 4.00 0.00 3.964 32 ACACAACACACAGTCAAGAAGCT 151 23 0 43.48 58.981 4.00 4.00 4.019 33 TACACAACAGACACACACACACAA 169 24 0 41.67 60.073 2.00 0.00 4.073 34 CACACAGTCAAGAAGCTACATCC 145 23 0 47.83 58.925 4.00 0.00 4.075 35 ACACAGTCAAGAAGCTACATCCA 144 23 0 43.48 58.925 4.00 0.00 4.075 36 TGCACAATACCACACATGTTTAA 102 23 0 34.78 58.925 6.00 4.00 4.075 37 ACACAACACACAGTCAAGAAGC 151 22 0 45.45 57.918 3.00 2.00 4.082 38 ATGCACAATACCACACATGTTT 103 22 0 36.36 57.811 6.00 6.00 4.189 39 ACACACAGTCAAGAAGCTACATCC 146 24 0 45.83 59.748 4.00 0.00 4.252 40 TGCACAATACCACACATGTTTAAC 102 24 0 37.50 59.733 6.00 5.00 4.267 41 GCACAATACCACACATGTTTAACA 101 24 0 37.50 59.733 7.00 7.00 4.267 42 TACACAACAGACACACACACACA 169 23 0 43.48 58.590 2.00 0.00 4.410 43 TGCACAATACCACACATGTTTA 102 22 0 36.36 57.504 6.00 4.00 4.496 44 GCTACATCCATACACAACACACA 131 23 0 43.48 58.499 2.00 0.00 4.501 45 AGCTACATCCATACACAACACACA 132 24 0 41.67 59.493 4.00 0.00 4.507 46 AGACACACACACACAACACACAGT 161 24 0 45.83 60.539 3.00 3.00 4.539 47 CACAGTCAAGAAGCTACATCCAT 143 23 0 43.48 58.408 4.00 2.00 4.592 48 ACACACACAACACACAGTCAAGAA 155 24 0 41.67 60.599 3.00 0.00 4.599 49 ACACACACACACAACACACAGT 159 22 0 45.45 57.352 3.00 3.00 4.648 50 CACAACACACATGTATATGCACAA 119 24 0 37.50 59.345 6.00 2.00 4.655 51 CAGACACACACACACAACACACA 162 23 0 47.83 61.701 1.00 0.00 4.701 52 CACACACACACAACACACAGTCA 158 23 0 47.83 61.701 3.00 1.00 4.701 53 CACACACAACACACAGTCAAGAAG 154 24 0 45.83 60.730 3.00 0.00 4.730 54 ACACAGTCAAGAAGCTACATCCAT 144 24 0 41.67 59.238 4.00 2.00 4.762 55 ATGCACAATACCACACATGTTTAA 103 24 0 33.33 59.233 6.00 4.00 4.767 56 CACAACACACAGTCAAGAAGCTA 150 23 0 43.48 58.160 4.00 2.00 4.840 57 CAACACACAGTCAAGAAGCTACA 148 23 0 43.48 58.160 4.00 2.00 4.840 58 GAAGCTACATCCATACACAACACA 134 24 0 41.67 59.091 4.00 0.00 4.909 59 ACACACACAACACACAGTCAAG 155 22 0 45.45 57.090 3.00 0.00 4.910 60 ACACAACAGACACACACACACAAC 168 24 0 45.83 60.929 1.00 0.00 4.929 61 ACAACAGACACACACACACAACAC 166 24 0 45.83 60.929 1.00 0.00 4.929 62 AACAGACACACACACACAACACAC 164 24 0 45.83 60.929 1.00 0.00 4.929 63 ACACAACACACAGTCAAGAAGCTA 151 24 0 41.67 59.030 4.00 2.00 4.970 64 CACAACACACAGTCAAGAAGCTAC 150 24 0 45.83 59.030 4.00 4.00 4.970 65 ACAACACACAGTCAAGAAGCTACA 149 24 0 41.67 59.030 4.00 2.00 4.970 66 AGAAGCTACATCCATACACAACACA 135 25 0 40.00 60.002 4.00 0.00 5.002 67 ATGCACAATACCACACATGTTTAAC 103 25 0 36.00 59.991 6.00 5.00 5.009 68 ATACACAACAGACACACACACACA 170 24 0 41.67 58.961 2.00 0.00 5.039 69 ACACACACACAACACACAGTCAAG 157 24 0 45.83 61.059 3.00 0.00 5.059 70 CACAACACACATGTATATGCACA 119 23 0 39.13 57.923 6.00 4.00 5.077 71 TATGCACAATACCACACATGTTT 104 23 0 34.78 57.903 6.00 6.00 5.097 72 ATGCACAATACCACACATGTTTA 103 23 0 34.78 57.903 6.00 4.00 5.097 73 GAAGCTACATCCATACACAACACAC 134 25 0 44.00 59.880 4.00 0.00 5.120 74 ACACAACACACATGTATATGCACAA 120 25 0 36.00 60.151 6.00 2.00 5.151 75 GCTACATCCATACACAACACACAT 131 24 0 41.67 58.842 2.00 2.00 5.158 76 ACACACAACACACAGTCAAGAAG 153 23 0 43.48 57.837 3.00 0.00 5.163 77 ACACAACACACAGTCAAGAAGCTAC 151 25 0 44.00 59.835 4.00 4.00 5.165 78 TCAAGAAGCTACATCCATACACAA 138 24 0 37.50 58.834 4.00 0.00 5.166 79 ACACAACACACATGTATATGCACA 120 24 0 37.50 58.828 6.00 4.00 5.172 80 AGCTACATCCATACACAACACACAT 132 25 0 40.00 59.772 4.00 2.00 5.228 81 CAACACACAGTCAAGAAGCTACATC 148 25 0 44.00 60.272 4.00 2.00 5.272 82 ATACACAACAGACACACACACACAA 170 25 0 40.00 60.344 2.00 0.00 5.344 83 ACATCCATACACAACACACATGT 128 23 0 39.13 57.646 6.00 6.00 5.354 84 CACAACACACATGTATATGCACAAT 119 25 0 36.00 59.637 6.00 2.00 5.363 85 GTCAAGAAGCTACATCCATACACAA 139 25 0 40.00 59.615 4.00 0.00 5.385 86 TCAAGAAGCTACATCCATACACAAC 138 25 0 40.00 59.615 4.00 0.00 5.385 87 CAAGAAGCTACATCCATACACAACA 137 25 0 40.00 60.458 4.00 0.00 5.458 88 CAACACACAGTCAAGAAGCTACAT 148 24 0 41.67 58.520 4.00 4.00 5.480 89 GACACACACACACAACACACAGTC 160 24 0 50.00 61.504 3.00 1.00 5.504 90 CACAGTCAAGAAGCTACATCCATA 143 24 0 41.67 58.476 4.00 2.00 5.524 91 CAGTCAAGAAGCTACATCCATACA 141 24 0 41.67 58.476 4.00 0.00 5.524 92 TCAAGAAGCTACATCCATACACA 138 23 0 39.13 57.467 4.00 0.00 5.533 93 GCACAATACCACACATGTTTAACAG 101 25 0 40.00 60.599 7.00 5.00 5.599 94 ACACACAACACACAGTCAAGAAGC 153 24 0 45.83 61.613 3.00 2.00 5.613 95 CACACAGTCAAGAAGCTACATCCA 145 24 0 45.83 61.619 4.00 0.00 5.619 96 GTCAAGAAGCTACATCCATACACA 139 24 0 41.67 58.339 4.00 0.00 5.661 97 ACAACACACAGTCAAGAAGCTACAT 149 25 0 40.00 59.333 4.00 4.00 5.667 98 CACACATGTATATGCACAATACCA 114 24 0 37.50 58.315 6.00 4.00 5.685 99 CACATGTATATGCACAATACCACA 112 24 0 37.50 58.315 6.00 2.00 5.685 100 CATGTATATGCACAATACCACACA 110 24 0 37.50 58.315 6.00 0.00 5.685 101 TGTATATGCACAATACCACACATG 108 24 0 37.50 58.315 6.00 4.00 5.685 102 ACACAGTCAAGAAGCTACATCCATA 144 25 0 40.00 59.274 4.00 2.00 5.726 103 CACAGTCAAGAAGCTACATCCATAC 143 25 0 44.00 59.274 4.00 0.00 5.726 104 ACAGTCAAGAAGCTACATCCATACA 142 25 0 40.00 59.274 4.00 0.00 5.726 105 CAGTCAAGAAGCTACATCCATACAC 141 25 0 44.00 59.274 4.00 0.00 5.726 106 AGTCAAGAAGCTACATCCATACACA 140 25 0 40.00 59.274 4.00 0.00 5.726 107 CACACAACACACAGTCAAGAAGCT 152 24 0 45.83 61.730 4.00 4.00 5.730 108 TATGCACAATACCACACATGTTTAA 104 25 0 32.00 59.268 6.00 4.00 5.732 109 ATATGCACAATACCACACATGTTT 105 24 0 33.33 58.262 6.00 6.00 5.738 110 AAGCTACATCCATACACAACACACA 133 25 0 40.00 60.742 4.00 0.00 5.742 111 AAGCTACATCCATACACAACACA 133 23 0 39.13 57.257 4.00 0.00 5.743 112 AAGCTACATCCATACACAACACAC 133 24 0 41.67 58.137 4.00 0.00 5.863 113 CCATACACAACACACATGTATATGC 124 25 0 40.00 59.136 6.00 4.00 5.864 114 ACACACATGTATATGCACAATACCA 115 25 0 36.00 59.136 6.00 4.00 5.864 115 CACACATGTATATGCACAATACCAC 114 25 0 40.00 59.136 6.00 2.00 5.864 116 ACACATGTATATGCACAATACCACA 113 25 0 36.00 59.136 6.00 2.00 5.864 117 CACATGTATATGCACAATACCACAC 112 25 0 40.00 59.136 6.00 0.00 5.864 118 ACATGTATATGCACAATACCACACA 111 25 0 36.00 59.136 6.00 0.00 5.864 119 TGTATATGCACAATACCACACATGT 108 25 0 36.00 59.136 6.00 6.00 5.864 120 TACACAACAGACACACACACACAAC 169 25 0 44.00 60.904 2.00 0.00 5.904 121 GCACAATACCACACATGTTTAAC 101 23 0 39.13 57.060 6.00 5.00 5.940 122 AACACACAGTCAAGAAGCTACATCC 147 25 0 44.00 60.962 4.00 0.00 5.962 123 CAAGAAGCTACATCCATACACAA 137 23 0 39.13 57.036 4.00 0.00 5.964 124 TATACACAACAGACACACACACACA 171 25 0 40.00 59.013 4.00 0.00 5.987 125 TATGCACAATACCACACATGTTTAAC 104 26 0 34.62 60.001 6.00 5.00 6.001 126 TATGCACAATACCACACATGTTTA 104 24 0 33.33 57.988 6.00 4.00 6.012 127 ACACAGTCAAGAAGCTACATCCATAC 144 26 0 42.31 60.018 4.00 0.00 6.018 128 ACAGTCAAGAAGCTACATCCATACAC 142 26 0 42.31 60.018 4.00 0.00 6.018 129 CACAATACCACACATGTTTAACAGA 100 25 0 36.00 58.912 7.00 3.00 6.088 130 CAAGAAGCTACATCCATACACAAC 137 24 0 41.67 57.904 4.00 0.00 6.096 131 AAGAAGCTACATCCATACACAACA 136 24 0 37.50 57.904 4.00 0.00 6.096 132 ACACACATGTATATGCACAATACCAC 115 26 0 38.46 59.900 6.00 2.00 6.100 133 ACACATGTATATGCACAATACCACAC 113 26 0 38.46 59.900 6.00 0.00 6.100 134 CTACATCCATACACAACACACATG 130 24 0 41.67 57.896 4.00 4.00 6.104 135 TACACAACACACATGTATATGCACA 121 25 0 36.00 58.882 6.00 4.00 6.118 136 CACACACACAACACACAGTCAAGA 156 24 0 45.83 62.141 3.00 2.00 6.141 137 TACACAACACACATGTATATGCACAA 121 26 0 34.62 60.156 6.00 2.00 6.156 138 CTACATCCATACACAACACACATGT 130 25 0 40.00 58.761 6.00 6.00 6.239 139 TACATCCATACACAACACACATGT 129 24 0 37.50 57.747 6.00 6.00 6.253 140 ACATCCATACACAACACACATGTA 128 24 0 37.50 57.747 6.00 4.00 6.253 141 AAGAAGCTACATCCATACACAACAC 136 25 0 40.00 58.712 4.00 0.00 6.288 142 CACAATACCACACATGTTTAACAGAC 100 26 0 38.46 59.680 7.00 1.00 6.320 143 CACAACACACATGTATATGCACAATA 119 26 0 34.62 59.659 6.00 4.00 6.341 144 TATACACAACAGACACACACACACAA 171 26 0 38.46 60.342 4.00 0.00 6.342 145 GTCAAGAAGCTACATCCATACACAAC 139 26 0 42.31 60.343 4.00 0.00 6.343 146 CAACACACATGTATATGCACAATACC 117 26 0 38.46 60.346 6.00 4.00 6.346 147 AACACACATGTATATGCACAATACCA 116 26 0 34.62 60.346 6.00 4.00 6.346 148 TGTATATGCACAATACCACACATGTT 108 26 0 34.62 60.346 6.00 4.00 6.346 149 CATGTATATGCACAATACCACACAT 110 25 0 36.00 58.650 6.00 2.00 6.350 150 ATGTATATGCACAATACCACACATG 109 25 0 36.00 58.650 6.00 4.00 6.350 151 AACACACAGTCAAGAAGCTACATC 147 24 0 41.67 57.626 4.00 2.00 6.374 152 ACACAACACACATGTATATGCACAAT 120 26 0 34.62 60.393 6.00 2.00 6.393 153 AGTCAAGAAGCTACATCCATACACAA 140 26 0 38.46 60.455 4.00 0.00 6.455 154 ATATGCACAATACCACACATGTTTAA 105 26 0 30.77 59.536 6.00 4.00 6.464 155 GCTACATCCATACACAACACACATG 131 25 0 44.00 61.472 4.00 4.00 6.472 156 ACACACACAACACACAGTCAAGAAG 155 25 0 44.00 61.508 3.00 0.00 6.508 157 ACAGACACACACACACAACACACA 163 24 0 45.83 62.520 1.00 0.00 6.520 158 ACACACACACACAACACACAGTCA 159 24 0 45.83 62.520 3.00 1.00 6.520 159 ACATGTATATGCACAATACCACACAT 111 26 0 34.62 59.420 6.00 2.00 6.580 160 ATGTATATGCACAATACCACACATGT 109 26 0 34.62 59.420 6.00 6.00 6.580 161 AGAAGCTACATCCATACACAACAC 135 24 0 41.67 57.400 4.00 0.00 6.600 162 CATACACAACACACATGTATATGCA 123 25 0 36.00 58.378 6.00 6.00 6.622 163 CAGACACACACACACAACACACAG 162 24 0 50.00 62.641 1.00 1.00 6.641 164 TATATGCACAATACCACACATGTTT 106 25 0 32.00 58.331 6.00 6.00 6.669 165 ATATGCACAATACCACACATGTTTA 105 25 0 32.00 58.331 6.00 4.00 6.669 166 ATATACACAACAGACACACACACACA 172 26 0 38.46 59.329 4.00 0.00 6.671 167 CACACAACACACAGTCAAGAAGCTA 152 25 0 44.00 61.678 4.00 2.00 6.678 168 CACAACACACAGTCAAGAAGCTACA 150 25 0 44.00 61.678 4.00 2.00 6.678 169 AGAAGCTACATCCATACACAACACAC 135 26 0 42.31 60.727 4.00 0.00 6.727 170 CATCCATACACAACACACATGTAT 127 24 0 37.50 57.241 6.00 6.00 6.759 171 CACACACACACAACATACACTCTAGA 222 26 0 42.31 59.211 6.00 6.00 6.789 172 CACACACACAACATACACTCTAGACA 220 26 0 42.31 59.211 6.00 2.00 6.789 173 CACACACAACATACACTCTAGACACA 218 26 0 42.31 59.211 6.00 0.00 6.789 174 CACACAGTCAAGAAGCTACATCCAT 145 25 0 44.00 61.791 4.00 2.00 6.791 175 CATACACAACACACATGTATATGCAC 123 26 0 38.46 59.190 6.00 4.00 6.810 176 ATACACAACACACATGTATATGCACA 122 26 0 34.62 59.190 6.00 4.00 6.810 177 TCCATACACAACACACATGTATATGC 125 26 0 38.46 60.811 6.00 4.00 6.811 178 CACAATACCACACATGTTTAACAG 100 24 0 37.50 57.140 7.00 5.00 6.860 179 ACATCCATACACAACACACATGTAT 128 25 0 36.00 58.124 6.00 6.00 6.876 180 ACACACACACACAACATACACTCT 223 24 0 41.67 57.096 2.00 1.00 6.904 181 GTATATGCACAATACCACACATGTTT 107 26 0 34.62 59.095 6.00 6.00 6.905 182 TCTACACACACACACAACATACACTC 226 26 0 42.31 59.077 2.00 0.00 6.923 183 AAGCTACATCCATACACAACACACAT 133 26 0 38.46 60.948 4.00 2.00 6.948 184 TATATGCACAATACCACACATGTT 106 24 0 33.33 57.002 6.00 4.00 6.998 185 TCTACACACACACACAACATACACTCT 226 27 0 40.74 60.001 2.00 1.00 7.001 186 ACACACACACACAACATACACTCTAGA 223 27 0 40.74 60.001 6.00 6.00 7.001 187 CACACACACACAACATACACTCTAGAC 222 27 0 44.44 60.001 6.00 4.00 7.001 188 ACACACACACAACATACACTCTAGACA 221 27 0 40.74 60.001 6.00 2.00 7.001 189 CACACACACAACATACACTCTAGACAC 220 27 0 44.44 60.001 6.00 0.00 7.001 190 ACACACACAACATACACTCTAGACACA 219 27 0 40.74 60.001 6.00 0.00 7.001 191 ACAACACACAGTCAAGAAGCTACATC 149 26 0 42.31 61.001 4.00 2.00 7.001 192 ATACACAACAGACACACACACACAAC 170 26 0 42.31 61.121 2.00 0.00 7.121 193 CATGTATATGCACAATACCACACATG 110 26 0 38.46 61.147 6.00 4.00 7.147 194 AACACACATGTATATGCACAATACC 116 25 0 36.00 57.851 6.00 4.00 7.149 195 GTATATGCACAATACCACACATGTT 107 25 0 36.00 57.851 6.00 4.00 7.149 196 CAAGAAGCTACATCCATACACAACAC 137 26 0 42.31 61.157 4.00 0.00 7.157 197 AAGAAGCTACATCCATACACAACACA 136 26 0 38.46 61.157 4.00 0.00 7.157 198 TACATCCATACACAACACACATGTA 129 25 0 36.00 57.841 6.00 4.00 7.159 199 CTACATCCATACACAACACACATGTA 130 26 0 38.46 58.815 6.00 4.00 7.185 200 ATATGCACAATACCACACATGTTTAAC 105 27 0 33.33 60.227 6.00 5.00 7.227 201 CATCCATACACAACACACATGTATATG 127 27 0 37.04 60.230 6.00 6.00 7.230 202 AACACACGTGTATATACACAACAGACA 183 27 0 37.04 59.748 12.00 1.00 7.252 203 TGCACAATACCACACATGTTTAACA 102 25 0 36.00 62.264 7.00 7.00 7.264 204 ACACACAGTCAAGAAGCTACATCCA 146 25 0 44.00 62.322 4.00 0.00 7.322 205 CACACGTGTATATACACAACAGACA 181 25 0 40.00 57.664 12.00 1.00 7.336 206 CACGTGTATATACACAACAGACACA 179 25 0 40.00 57.664 12.00 1.00 7.336 207 CGTGTATATACACAACAGACACACA 177 25 0 40.00 57.664 12.00 0.00 7.336 208 ATACACAACACACATGTATATGCACAA 122 27 0 33.33 60.389 6.00 2.00 7.389 209 TACACAACACACATGTATATGCACAAT 121 27 0 33.33 60.389 6.00 2.00 7.389 210 ACACAACACACATGTATATGCACAATA 120 27 0 33.33 60.389 6.00 4.00 7.389 211 CACAACACACATGTATATGCACAATAC 119 27 0 37.04 60.389 6.00 6.00 7.389 212 AGACACACACACACAACACACAGTC 161 25 0 48.00 62.414 3.00 1.00 7.414 213 TATATGCACAATACCACACATGTTTAA 106 27 0 29.63 59.560 6.00 4.00 7.440 214 ACACACAACACACAGTCAAGAAGCT 153 25 0 44.00 62.443 4.00 4.00 7.443 215 GATCTACACACACACACAACATACA 228 25 0 40.00 57.548 4.00 0.00 7.452 216 ACACACGTGTATATACACAACAGACA 182 26 0 38.46 58.508 12.00 1.00 7.492 217 CACACGTGTATATACACAACAGACAC 181 26 0 42.31 58.508 12.00 3.00 7.492 218 ACACGTGTATATACACAACAGACACA 180 26 0 38.46 58.508 12.00 1.00 7.492 219 CACGTGTATATACACAACAGACACAC 179 26 0 42.31 58.508 12.00 1.00 7.492 220 ACGTGTATATACACAACAGACACACA 178 26 0 38.46 58.508 12.00 0.00 7.492 221 CGTGTATATACACAACAGACACACAC 177 26 0 42.31 58.508 12.00 0.00 7.492 222 CACACACAACATACACTCTAGACACAT 218 27 0 40.74 59.502 6.00 2.00 7.498 223 ATGTATATGCACAATACCACACATGTT 109 27 0 33.33 60.562 6.00 4.00 7.562 224 GATCTACACACACACACAACATACAC 228 26 0 42.31 58.431 4.00 0.00 7.569 225 ATATACACAACAGACACACACACACAA 172 27 0 37.04 60.577 4.00 0.00 7.577 226 TATATGCACAATACCACACATGTTTA 106 26 0 30.77 58.395 6.00 4.00 7.605 227 GATCTACACACACACACAACATACACT 228 27 0 40.74 59.376 4.00 1.00 7.624 228 ATCTACACACACACACAACATACACTC 227 27 0 40.74 59.376 2.00 0.00 7.624 229 CACACACACACAACATACACTCTAG 222 25 0 44.00 57.370 4.00 4.00 7.630 230 TGTATATACACAACAGACACACACACA 175 27 0 37.04 59.363 10.00 0.00 7.637 231 TATATACACAACAGACACACACACACA 173 27 0 37.04 59.363 6.00 0.00 7.637 232 CATCCATACACAACACACATGTATA 127 25 0 36.00 57.352 6.00 4.00 7.648 233 TCCATACACAACACACATGTATATG 125 25 0 36.00 57.352 6.00 6.00 7.648 234 CCATACACAACACACATGTATATGCA 124 26 0 38.46 61.654 6.00 6.00 7.654 235 CACACATGTATATGCACAATACCACA 114 26 0 38.46 61.654 6.00 2.00 7.654 236 CACATGTATATGCACAATACCACACA 112 26 0 38.46 61.654 6.00 0.00 7.654 237 ACACACGTGTATATACACAACAGACAC 182 27 0 40.74 59.293 12.00 3.00 7.707 238 ACACGTGTATATACACAACAGACACAC 180 27 0 40.74 59.293 12.00 1.00 7.707 239 ACGTGTATATACACAACAGACACACAC 178 27 0 40.74 59.293 12.00 0.00 7.707 240 CACACAGTCAAGAAGCTACATCCATA 145 26 0 42.31 61.740 4.00 2.00 7.740 241 CACAGTCAAGAAGCTACATCCATACA 143 26 0 42.31 61.740 4.00 0.00 7.740 242 CAGTCAAGAAGCTACATCCATACACA 141 26 0 42.31 61.740 4.00 0.00 7.740 243 CTACACACACACACAACATACACTCT 225 26 0 42.31 58.251 2.00 1.00 7.749 244 ACACACACACACAACATACACTCTAG 223 26 0 42.31 58.251 4.00 4.00 7.749 245 TCTACACACACACACAACATACACT 226 25 0 40.00 57.217 2.00 1.00 7.783 246 CTACACACACACACAACATACACTC 225 25 0 44.00 57.217 2.00 0.00 7.783 247 TACACACACACACAACATACACTCT 224 25 0 40.00 57.217 2.00 1.00 7.783 248 ACACACACACACAACATACACTCTA 223 25 0 40.00 57.217 3.00 3.00 7.783 249 TACATCCATACACAACACACATGTAT 129 26 0 34.62 58.200 6.00 6.00 7.800 250 ACATCCATACACAACACACATGTATA 128 26 0 34.62 58.200 6.00 4.00 7.800 251 CACAACACACAGTCAAGAAGCTACAT 150 26 0 42.31 61.844 4.00 4.00 7.844 252 GAACACACGTGTATATACACAACAGA 184 26 0 38.46 58.139 12.00 4.00 7.861 253 GTATATGCACAATACCACACATGTTTA 107 27 0 33.33 59.133 6.00 4.00 7.867 254 CTACATCCATACACAACACACATGTAT 130 27 0 37.04 59.113 6.00 6.00 7.887 255 ACACACACACAACACACAGTCAAGA 157 25 0 44.00 62.889 3.00 2.00 7.889 256 ACAACACACATGTATATGCACAATA 118 25 0 32.00 57.073 6.00 4.00 7.927 257 CAACACACATGTATATGCACAATAC 117 25 0 36.00 57.073 6.00 6.00 7.927 258 AGAACACACGTGTATATACACAACAGA 185 27 0 37.04 59.049 12.00 4.00 7.951 259 ATCCATACACAACACACATGTATATGC 126 27 0 37.04 61.010 6.00 4.00 8.010 260 ACAACACACATGTATATGCACAATACC 118 27 0 37.04 61.034 6.00 4.00 8.034 261 AACACACATGTATATGCACAATACCAC 116 27 0 37.04 61.034 6.00 2.00 8.034 262 CACACGTGTATATACACAACAGACACA 181 27 0 40.74 61.042 12.00 1.00 8.042 263 CACGTGTATATACACAACAGACACACA 179 27 0 40.74 61.042 12.00 0.00 8.042 264 CGTGTATATACACAACAGACACACACA 177 27 0 40.74 61.042 12.00 0.00 8.042 265 TCAAGAAGCTACATCCATACACAACA 138 26 0 38.46 62.056 4.00 0.00 8.056 266 GAACACACGTGTATATACACAACAGAC 184 27 0 40.74 58.926 12.00 2.00 8.074 267 ACAACACACATGTATATGCACAATAC 118 26 0 34.62 57.913 6.00 6.00 8.087 268 TATACACAACAGACACACACACACAAC 171 27 0 40.74 61.092 4.00 0.00 8.092 269 AGTCAAGAAGCTACATCCATACACAAC 140 27 0 40.74 61.126 4.00 0.00 8.126 270 GCTACATCCATACACAACACACATGT 131 26 0 42.31 62.163 6.00 6.00 8.163 271 GCACAATACCACACATGTTTAACAGA 101 26 0 38.46 62.185 7.00 3.00 8.185 272 AGCTACATCCATACACAACACACATG 132 26 0 42.31 62.266 4.00 4.00 8.266 273 CATCCATACACAACACACATGTATAT 127 26 0 34.62 57.730 6.00 6.00 8.270 274 ATCCATACACAACACACATGTATATG 126 26 0 34.62 57.730 6.00 6.00 8.270 275 ACACACAACACACAGTCAAGAAGCTA 153 26 0 42.31 62.367 4.00 2.00 8.367 276 CACACAACACACAGTCAAGAAGCTAC 152 26 0 46.15 62.367 4.00 4.00 8.367 277 ACACAACACACAGTCAAGAAGCTACA 151 26 0 42.31 62.367 4.00 2.00 8.367 278 GAAGCTACATCCATACACAACACACA 134 26 0 42.31 62.368 4.00 0.00 8.368 279 ATCTACACACACACACAACATACACT 227 26 0 38.46 57.620 2.00 1.00 8.380 280 ATGCACAATACCACACATGTTTAACA 103 26 0 34.62 62.397 7.00 7.00 8.397 281 TGTATATGCACAATACCACACATGTTT 108 27 0 33.33 61.445 6.00 6.00 8.445 282 ACACACAGTCAAGAAGCTACATCCAT 146 26 0 42.31 62.454 4.00 2.00 8.454 283 ACATCCATACACAACACACATGTATAT 128 27 0 33.33 58.526 6.00 6.00 8.474 284 AGAACACACGTGTATATACACAACAG 185 26 0 38.46 57.375 12.00 6.00 8.625 285 ACACACACACAACATACACTCTAGAC 221 26 0 42.31 57.364 6.00 4.00 8.636 286 ACACACACAACATACACTCTAGACAC 219 26 0 42.31 57.364 6.00 0.00 8.636 287 TACACACACACACAACATACACTCTA 224 26 0 38.46 57.330 3.00 3.00 8.670 288 CTACACACACACACAACATACACTCTA 225 27 0 40.74 58.323 3.00 3.00 8.677 289 TACACACACACACAACATACACTCTAG 224 27 0 40.74 58.323 4.00 4.00 8.677 290 CATACACAACACACATGTATATGCACA 123 27 0 37.04 61.688 6.00 4.00 8.688 291 TACATCCATACACAACACACATGTATA 129 27 0 33.33 58.271 6.00 4.00 8.729 292 AACACACGTGTATATACACAACAGAC 183 26 0 38.46 57.237 12.00 2.00 8.763 293 AAGAAGCTACATCCATACACAACACAC 136 27 0 40.74 61.811 4.00 0.00 8.811 294 CACATGTATATGCACAATACCACACAT 112 27 0 37.04 61.814 6.00 2.00 8.814 295 ACATGTATATGCACAATACCACACATG 111 27 0 37.04 61.814 6.00 4.00 8.814 296 CATGTATATGCACAATACCACACATGT 110 27 0 37.04 61.814 6.00 6.00 8.814 297 CACAATACCACACATGTTTAACAGACA 100 27 0 37.04 62.089 7.00 3.00 9.089 298 GCTACATCCATACACAACACACATGTA 131 27 0 40.74 62.100 6.00 4.00 9.100 299 CCATACACAACACACATGTATATGCAC 124 27 0 40.74 62.312 6.00 4.00 9.312 300 ACACACATGTATATGCACAATACCACA 115 27 0 37.04 62.312 6.00 2.00 9.312 301 CACACATGTATATGCACAATACCACAC 114 27 0 40.74 62.312 6.00 0.00 9.312 302 ACACATGTATATGCACAATACCACACA 113 27 0 37.04 62.312 6.00 0.00 9.312 303 TATGCACAATACCACACATGTTTAACA 104 27 0 33.33 62.329 7.00 7.00 9.329 304 ACACACAGTCAAGAAGCTACATCCATA 146 27 0 40.74 62.383 4.00 2.00 9.383 305 CACACAGTCAAGAAGCTACATCCATAC 145 27 0 44.44 62.383 4.00 0.00 9.383 306 ACACAGTCAAGAAGCTACATCCATACA 144 27 0 40.74 62.383 4.00 0.00 9.383 307 CACAGTCAAGAAGCTACATCCATACAC 143 27 0 44.44 62.383 4.00 0.00 9.383 308 ACAGTCAAGAAGCTACATCCATACACA 142 27 0 40.74 62.383 4.00 0.00 9.383 309 GTGTATATACACAACAGACACACACAC 176 27 0 40.74 57.575 12.00 0.00 9.425 310 GTATATACACAACAGACACACACACAC 174 27 0 40.74 57.575 8.00 0.00 9.425 311 GAAGCTACATCCATACACAACACACAT 134 27 0 40.74 62.494 4.00 2.00 9.494 312 ACACAACACACAGTCAAGAAGCTACAT 151 27 0 40.74 62.495 4.00 4.00 9.495 313 TAGAACACACGTGTATATACACAACAG 186 27 0 37.04 57.472 12.00 6.00 9.528 314 GTCAAGAAGCTACATCCATACACAACA 139 27 0 40.74 62.685 4.00 0.00 9.685 315 TCAAGAAGCTACATCCATACACAACAC 138 27 0 40.74 62.685 4.00 0.00 9.685 316 CAACACACATGTATATGCACAATACCA 117 27 0 37.04 62.716 6.00 4.00 9.716 317 CAGTCAAGAAGCTACATCCATACACAA 141 27 0 40.74 62.778 4.00 0.00 9.778 318 GCACAATACCACACATGTTTAACAGAC 101 27 0 40.74 62.806 7.00 1.00 9.806 319 AGCTACATCCATACACAACACACATGT 132 27 0 40.74 62.902 6.00 6.00 9.902 primer3-2.3.6/test/primer1_list_last/SP01-19.txt.rev0000664000077300007730000001063112231156243020737 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TCCCTCATCCAGTGCTCTTT 185 20 0 50.00 59.803 4.00 0.00 0.197 1 CCCTCATCCAGTGCTCTTTC 184 20 0 55.00 59.803 4.00 0.00 0.197 2 CAGTGCTCTTTCTGCCAGTG 176 20 0 55.00 59.770 5.00 3.00 0.230 3 CCAGTGCTCTTTCTGCCAGT 177 20 0 55.00 60.589 5.00 3.00 0.589 4 TGCTCTTTCTGCCAGTGTCA 173 20 0 50.00 60.745 5.00 5.00 0.745 5 GATCCCTCATCCAGTGCTCT 187 20 0 55.00 59.210 4.00 4.00 0.790 6 TCATCCAGTGCTCTTTCTGC 181 20 0 50.00 59.116 4.00 2.00 0.884 7 CATCCAGTGCTCTTTCTGCC 180 20 0 55.00 60.945 4.00 1.00 0.945 8 ATCCAGTGCTCTTTCTGCCA 179 20 0 50.00 60.945 4.00 3.00 0.945 9 CCTCATCCAGTGCTCTTTCTG 183 21 0 52.38 59.997 4.00 2.00 1.003 10 ATCCCTCATCCAGTGCTCTTT 186 21 0 47.62 60.088 4.00 0.00 1.088 11 CTCATCCAGTGCTCTTTCTGC 182 21 0 52.38 60.149 4.00 2.00 1.149 12 CCAGTGCTCTTTCTGCCAG 177 19 0 57.89 59.710 5.00 5.00 1.290 13 ATCCCTCATCCAGTGCTCTT 186 20 0 50.00 58.693 4.00 2.00 1.307 14 AGTGCTCTTTCTGCCAGTGTC 175 21 0 52.38 59.661 5.00 3.00 1.339 15 GTGCTCTTTCTGCCAGTGTC 174 20 0 55.00 58.596 5.00 3.00 1.404 16 GCTCTTTCTGCCAGTGTCAC 172 20 0 55.00 58.596 5.00 5.00 1.404 17 GATCCCTCATCCAGTGCTCTT 187 21 0 52.38 60.611 4.00 2.00 1.611 18 CAGTGCTCTTTCTGCCAGTGT 176 21 0 52.38 60.638 5.00 1.00 1.638 19 TCCAGTGCTCTTTCTGCCAG 178 20 0 55.00 61.674 5.00 5.00 1.674 20 TCCAGTGCTCTTTCTGCCA 178 19 0 52.63 60.694 4.00 3.00 1.694 21 CCCTCATCCAGTGCTCTTTCT 184 21 0 52.38 60.771 4.00 0.00 1.771 22 GGCCCTCTGGTTAACCGT 149 18 0 61.11 59.927 8.00 4.00 2.073 23 AGTGCTCTTTCTGCCAGTGT 175 20 0 50.00 57.670 5.00 1.00 2.330 24 GTGCTCTTTCTGCCAGTGTCA 174 21 0 52.38 61.588 5.00 5.00 2.588 25 TGCTCTTTCTGCCAGTGTCAC 173 21 0 52.38 61.588 5.00 5.00 2.588 26 TCCCTCATCCAGTGCTCTTTC 185 21 0 52.38 61.686 4.00 0.00 2.686 27 TCCCTCATCCAGTGCTCTT 185 19 0 52.63 58.306 4.00 2.00 2.694 28 ATCCAGTGCTCTTTCTGCCAG 179 21 0 52.38 61.860 5.00 5.00 2.860 29 GCCCTCTGGTTAACCGTG 148 18 0 61.11 59.086 8.00 6.00 2.914 30 GATCCCTCATCCAGTGCTC 187 19 0 57.89 58.080 4.00 2.00 2.920 31 CCTCATCCAGTGCTCTTTCT 183 20 0 50.00 57.032 4.00 0.00 2.968 32 ATCCAGTGCTCTTTCTGCC 179 19 0 52.63 57.954 4.00 1.00 3.046 33 CCCTCATCCAGTGCTCTTT 184 19 0 52.63 57.795 4.00 0.00 3.205 34 TGCTCTTTCTGCCAGTGTC 173 19 0 52.63 57.590 5.00 3.00 3.410 35 GCTCTTTCTGCCAGTGTCA 172 19 0 52.63 57.590 5.00 5.00 3.410 36 CCAGTGCTCTTTCTGCCA 177 18 0 55.56 58.588 4.00 3.00 3.412 37 TCCAGTGCTCTTTCTGCCAGT 178 21 0 52.38 62.447 5.00 3.00 3.447 38 TTGGCCCTCTGGTTAACC 151 18 0 55.56 58.487 8.00 8.00 3.513 39 TGGCCCTCTGGTTAACCGT 150 19 0 57.89 62.761 8.00 4.00 3.761 40 GGCCCTCTGGTTAACCGTG 149 19 0 63.16 62.761 8.00 6.00 3.761 41 TCATCCAGTGCTCTTTCTGCC 181 21 0 52.38 62.794 4.00 1.00 3.794 42 GATCCCTCATCCAGTGCTCTTT 187 22 0 50.00 61.864 4.00 0.00 3.864 43 ATCCCTCATCCAGTGCTCTTTC 186 22 0 50.00 61.864 4.00 0.00 3.864 44 ATCCCTCATCCAGTGCTCT 186 19 0 52.63 57.128 4.00 4.00 3.872 45 CATCCAGTGCTCTTTCTGC 180 19 0 52.63 57.014 4.00 2.00 3.986 46 TGGCCCTCTGGTTAACCG 150 18 0 61.11 62.001 8.00 6.00 4.001 47 GTGCTCTTTCTGCCAGTGTCAC 174 22 0 54.55 62.364 5.00 5.00 4.364 48 CAGTGCTCTTTCTGCCAGTGTC 176 22 0 54.55 62.479 5.00 3.00 4.479 49 AGTGCTCTTTCTGCCAGTGTCA 175 22 0 50.00 62.479 5.00 5.00 4.479 50 TCCAGTGCTCTTTCTGCC 178 18 0 55.56 57.507 4.00 1.00 4.493 51 TCCCTCATCCAGTGCTCTTTCT 185 22 0 50.00 62.539 4.00 0.00 4.539 52 ATCCAGTGCTCTTTCTGCCAGT 179 22 0 50.00 62.585 5.00 3.00 4.585 53 ATCCCTCATCCAGTGCTCTTTCT 186 23 0 47.83 62.667 4.00 0.00 5.667 primer3-2.3.6/test/primer1_list_last/included_test_w_middle.int0000664000077300007730000007763312231156243023716 0ustar ioanaioanaACCEPTABLE INTERNAL OLIGOS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGTATGTTGTGTGTGTGTGTAGATC 224 25 0 40.00 57.548 4.00 4.00 7.452 1 GTGTATGTTGTGTGTGTGTGTAGATC 223 26 0 42.31 58.431 4.00 4.00 7.569 2 AGTGTATGTTGTGTGTGTGTGTAGATC 222 27 0 40.74 59.376 4.00 4.00 7.624 3 AGTGTATGTTGTGTGTGTGTGTAGAT 222 26 0 38.46 57.620 2.00 2.00 8.380 4 GAGTGTATGTTGTGTGTGTGTGTAGAT 221 27 0 40.74 59.376 2.00 2.00 7.624 5 AGTGTATGTTGTGTGTGTGTGTAGA 222 25 0 40.00 57.217 2.00 2.00 7.783 6 GAGTGTATGTTGTGTGTGTGTGTAGA 221 26 0 42.31 59.077 2.00 2.00 6.923 7 AGAGTGTATGTTGTGTGTGTGTGTAGA 220 27 0 40.74 60.001 2.00 2.00 7.001 8 GAGTGTATGTTGTGTGTGTGTGTAG 221 25 0 44.00 57.217 2.00 0.00 7.783 9 AGAGTGTATGTTGTGTGTGTGTGTAG 220 26 0 42.31 58.251 2.00 0.00 7.749 10 TAGAGTGTATGTTGTGTGTGTGTGTAG 219 27 0 40.74 58.323 3.00 0.00 8.677 11 AGAGTGTATGTTGTGTGTGTGTGTA 220 25 0 40.00 57.217 2.00 2.00 7.783 12 TAGAGTGTATGTTGTGTGTGTGTGTA 219 26 0 38.46 57.330 3.00 2.00 8.670 13 CTAGAGTGTATGTTGTGTGTGTGTGTA 218 27 0 40.74 58.323 4.00 2.00 8.677 14 AGAGTGTATGTTGTGTGTGTGTGT 220 24 0 41.67 57.096 2.00 0.00 6.904 15 TAGAGTGTATGTTGTGTGTGTGTGT 219 25 0 40.00 57.217 3.00 0.00 7.783 16 CTAGAGTGTATGTTGTGTGTGTGTGT 218 26 0 42.31 58.251 4.00 0.00 7.749 17 TCTAGAGTGTATGTTGTGTGTGTGTGT 217 27 0 40.74 60.001 6.00 0.00 7.001 18 CTAGAGTGTATGTTGTGTGTGTGTG 218 25 0 44.00 57.370 4.00 0.00 7.630 19 TCTAGAGTGTATGTTGTGTGTGTGTG 217 26 0 42.31 59.211 6.00 0.00 6.789 20 GTCTAGAGTGTATGTTGTGTGTGTGTG 216 27 0 44.44 60.001 6.00 0.00 7.001 21 GTCTAGAGTGTATGTTGTGTGTGTGT 216 26 0 42.31 57.364 6.00 0.00 8.636 22 TGTCTAGAGTGTATGTTGTGTGTGTGT 215 27 0 40.74 60.001 6.00 0.00 7.001 23 TGTCTAGAGTGTATGTTGTGTGTGTG 215 26 0 42.31 59.211 6.00 0.00 6.789 24 GTGTCTAGAGTGTATGTTGTGTGTGTG 214 27 0 44.44 60.001 6.00 0.00 7.001 25 GTGTCTAGAGTGTATGTTGTGTGTGT 214 26 0 42.31 57.364 6.00 0.00 8.636 26 TGTGTCTAGAGTGTATGTTGTGTGTGT 213 27 0 40.74 60.001 6.00 0.00 7.001 27 TGTGTCTAGAGTGTATGTTGTGTGTG 213 26 0 42.31 59.211 6.00 0.00 6.789 28 CTGTGTCTAGAGTGTATGTTGTGTGTG 212 27 0 44.44 60.122 6.00 0.00 7.122 29 CTGTGTCTAGAGTGTATGTTGTGTGT 212 26 0 42.31 57.508 6.00 0.00 8.492 30 TGTGTATATACACGTGTGTTCTATGTC 184 27 0 37.04 57.285 12.00 2.00 9.715 31 CTGTTGTGTATATACACGTGTGTTCTA 180 27 0 37.04 57.472 12.00 6.00 9.528 32 CTGTTGTGTATATACACGTGTGTTCT 180 26 0 38.46 57.375 12.00 4.00 8.625 33 TCTGTTGTGTATATACACGTGTGTTCT 179 27 0 37.04 59.049 12.00 4.00 7.951 34 TCTGTTGTGTATATACACGTGTGTTC 179 26 0 38.46 58.139 12.00 4.00 7.861 35 GTCTGTTGTGTATATACACGTGTGTTC 178 27 0 40.74 58.926 12.00 4.00 8.074 36 GTCTGTTGTGTATATACACGTGTGTT 178 26 0 38.46 57.237 12.00 6.00 8.763 37 TGTCTGTTGTGTATATACACGTGTGTT 177 27 0 37.04 59.748 12.00 6.00 7.252 38 TGTCTGTTGTGTATATACACGTGTGT 177 26 0 38.46 58.508 12.00 8.00 7.492 39 GTGTCTGTTGTGTATATACACGTGTGT 176 27 0 40.74 59.293 12.00 8.00 7.707 40 TGTCTGTTGTGTATATACACGTGTG 177 25 0 40.00 57.664 12.00 6.00 7.336 41 GTGTCTGTTGTGTATATACACGTGTG 176 26 0 42.31 58.508 12.00 6.00 7.492 42 TGTGTCTGTTGTGTATATACACGTGTG 175 27 0 40.74 61.042 12.00 6.00 8.042 43 TGTGTCTGTTGTGTATATACACGTGT 175 26 0 38.46 58.508 12.00 8.00 7.492 44 GTGTGTCTGTTGTGTATATACACGTGT 174 27 0 40.74 59.293 12.00 8.00 7.707 45 TGTGTCTGTTGTGTATATACACGTG 175 25 0 40.00 57.664 12.00 6.00 7.336 46 GTGTGTCTGTTGTGTATATACACGTG 174 26 0 42.31 58.508 12.00 6.00 7.492 47 TGTGTGTCTGTTGTGTATATACACGTG 173 27 0 40.74 61.042 12.00 6.00 8.042 48 TGTGTGTCTGTTGTGTATATACACGT 173 26 0 38.46 58.508 12.00 8.00 7.492 49 GTGTGTGTCTGTTGTGTATATACACGT 172 27 0 40.74 59.293 12.00 8.00 7.707 50 GTGTGTGTGTGTCTGTTGTGTATATAC 168 27 0 40.74 57.575 8.00 8.00 9.425 51 TGTGTGTGTGTGTCTGTTGTGTATATA 167 27 0 37.04 59.363 6.00 6.00 7.637 52 TGTGTGTGTGTGTCTGTTGTGTATAT 167 26 0 38.46 59.329 4.00 4.00 6.671 53 TTGTGTGTGTGTGTCTGTTGTGTATAT 166 27 0 37.04 60.577 4.00 4.00 7.577 54 TGTGTGTGTGTGTCTGTTGTGTATA 167 25 0 40.00 59.013 4.00 4.00 5.987 55 TTGTGTGTGTGTGTCTGTTGTGTATA 166 26 0 38.46 60.342 4.00 4.00 6.342 56 GTTGTGTGTGTGTGTCTGTTGTGTATA 165 27 0 40.74 61.092 4.00 4.00 8.092 57 TGTGTGTGTGTGTCTGTTGTGTAT 167 24 0 41.67 58.961 2.00 2.00 5.039 58 TTGTGTGTGTGTGTCTGTTGTGTAT 166 25 0 40.00 60.344 2.00 2.00 5.344 59 GTTGTGTGTGTGTGTCTGTTGTGTAT 165 26 0 42.31 61.121 2.00 2.00 7.121 60 TGTGTGTGTGTGTCTGTTGTGTA 167 23 0 43.48 58.590 2.00 2.00 4.410 61 TTGTGTGTGTGTGTCTGTTGTGTA 166 24 0 41.67 60.073 2.00 2.00 4.073 62 GTTGTGTGTGTGTGTCTGTTGTGTA 165 25 0 44.00 60.904 2.00 2.00 5.904 63 TGTGTGTGTGTGTCTGTTGTGT 167 22 0 45.45 58.514 1.00 0.00 3.486 64 TTGTGTGTGTGTGTCTGTTGTGT 166 23 0 43.48 60.064 1.00 0.00 3.064 65 GTTGTGTGTGTGTGTCTGTTGTGT 165 24 0 45.83 60.929 1.00 0.00 4.929 66 TGTGTGTGTGTGTCTGTTGTG 167 21 0 47.62 57.434 1.00 0.00 3.566 67 TTGTGTGTGTGTGTCTGTTGTG 166 22 0 45.45 59.121 1.00 0.00 2.879 68 GTTGTGTGTGTGTGTCTGTTGTG 165 23 0 47.83 60.064 1.00 0.00 3.064 69 TGTTGTGTGTGTGTGTCTGTTGT 164 23 0 43.48 60.064 1.00 0.00 3.064 70 GTGTTGTGTGTGTGTGTCTGTTGT 163 24 0 45.83 60.929 1.00 0.00 4.929 71 TGTTGTGTGTGTGTGTCTGTTG 164 22 0 45.45 59.121 1.00 0.00 2.879 72 GTGTTGTGTGTGTGTGTCTGTTG 163 23 0 47.83 60.064 1.00 0.00 3.064 73 TGTGTTGTGTGTGTGTGTCTGTT 162 23 0 43.48 60.064 1.00 0.00 3.064 74 GTGTGTTGTGTGTGTGTGTCTGTT 161 24 0 45.83 60.929 1.00 0.00 4.929 75 TGTGTTGTGTGTGTGTGTCTGT 162 22 0 45.45 58.514 1.00 0.00 3.486 76 GTGTGTTGTGTGTGTGTGTCTGT 161 23 0 47.83 59.499 1.00 0.00 3.501 77 TGTGTGTTGTGTGTGTGTGTCTGT 160 24 0 45.83 62.520 1.00 0.00 6.520 78 TGTGTTGTGTGTGTGTGTCTG 162 21 0 47.62 57.434 1.00 1.00 3.566 79 GTGTGTTGTGTGTGTGTGTCTG 161 22 0 50.00 58.514 1.00 1.00 3.486 80 TGTGTGTTGTGTGTGTGTGTCTG 160 23 0 47.83 61.701 1.00 1.00 4.701 81 CTGTGTGTTGTGTGTGTGTGTCTG 159 24 0 50.00 62.641 1.00 1.00 6.641 82 TGTGTGTTGTGTGTGTGTGTCT 160 22 0 45.45 58.514 1.00 0.00 3.486 83 CTGTGTGTTGTGTGTGTGTGTCT 159 23 0 47.83 59.650 1.00 0.00 3.350 84 ACTGTGTGTTGTGTGTGTGTGTCT 158 24 0 45.83 60.539 3.00 0.00 4.539 85 GACTGTGTGTTGTGTGTGTGTGTCT 157 25 0 48.00 62.414 3.00 0.00 7.414 86 TGTGTGTTGTGTGTGTGTGTC 160 21 0 47.62 57.244 1.00 1.00 3.756 87 CTGTGTGTTGTGTGTGTGTGTC 159 22 0 50.00 58.514 1.00 1.00 3.486 88 ACTGTGTGTTGTGTGTGTGTGTC 158 23 0 47.83 59.499 3.00 1.00 3.501 89 GACTGTGTGTTGTGTGTGTGTGTC 157 24 0 50.00 61.504 3.00 1.00 5.504 90 ACTGTGTGTTGTGTGTGTGTGT 158 22 0 45.45 57.352 3.00 0.00 4.648 91 GACTGTGTGTTGTGTGTGTGTGT 157 23 0 47.83 59.499 3.00 0.00 3.501 92 TGACTGTGTGTTGTGTGTGTGTGT 156 24 0 45.83 62.520 3.00 0.00 6.520 93 GACTGTGTGTTGTGTGTGTGTG 157 22 0 50.00 58.514 3.00 0.00 3.486 94 TGACTGTGTGTTGTGTGTGTGTG 156 23 0 47.83 61.701 3.00 0.00 4.701 95 TGACTGTGTGTTGTGTGTGTGT 156 22 0 45.45 58.514 3.00 0.00 3.486 96 TTGACTGTGTGTTGTGTGTGTGT 155 23 0 43.48 60.064 3.00 0.00 3.064 97 CTTGACTGTGTGTTGTGTGTGTGT 154 24 0 45.83 61.059 3.00 0.00 5.059 98 TCTTGACTGTGTGTTGTGTGTGTGT 153 25 0 44.00 62.889 3.00 0.00 7.889 99 TGACTGTGTGTTGTGTGTGTG 156 21 0 47.62 57.434 3.00 0.00 3.566 100 TTGACTGTGTGTTGTGTGTGTG 155 22 0 45.45 59.121 3.00 0.00 2.879 101 CTTGACTGTGTGTTGTGTGTGTG 154 23 0 47.83 60.208 3.00 0.00 3.208 102 TCTTGACTGTGTGTTGTGTGTGTG 153 24 0 45.83 62.141 3.00 0.00 6.141 103 CTTGACTGTGTGTTGTGTGTGT 154 22 0 45.45 57.090 3.00 0.00 4.910 104 TCTTGACTGTGTGTTGTGTGTGT 153 23 0 43.48 59.188 3.00 0.00 3.812 105 TTCTTGACTGTGTGTTGTGTGTGT 152 24 0 41.67 60.599 3.00 0.00 4.599 106 CTTCTTGACTGTGTGTTGTGTGTGT 151 25 0 44.00 61.508 3.00 0.00 6.508 107 TCTTGACTGTGTGTTGTGTGTG 153 22 0 45.45 58.217 3.00 0.00 3.783 108 TTCTTGACTGTGTGTTGTGTGTG 152 23 0 43.48 59.744 3.00 0.00 3.256 109 CTTCTTGACTGTGTGTTGTGTGTG 151 24 0 45.83 60.730 3.00 0.00 4.730 110 CTTCTTGACTGTGTGTTGTGTGT 151 23 0 43.48 57.837 3.00 0.00 5.163 111 GCTTCTTGACTGTGTGTTGTGTGT 150 24 0 45.83 61.613 3.00 0.00 5.613 112 AGCTTCTTGACTGTGTGTTGTGTGT 149 25 0 44.00 62.443 4.00 0.00 7.443 113 TAGCTTCTTGACTGTGTGTTGTGTGT 148 26 0 42.31 62.367 4.00 0.00 8.367 114 GCTTCTTGACTGTGTGTTGTGTG 150 23 0 47.83 60.832 3.00 0.00 3.832 115 AGCTTCTTGACTGTGTGTTGTGTG 149 24 0 45.83 61.730 4.00 0.00 5.730 116 TAGCTTCTTGACTGTGTGTTGTGTG 148 25 0 44.00 61.678 4.00 0.00 6.678 117 GTAGCTTCTTGACTGTGTGTTGTGTG 147 26 0 46.15 62.367 4.00 0.00 8.367 118 GCTTCTTGACTGTGTGTTGTGT 150 22 0 45.45 57.918 3.00 0.00 4.082 119 AGCTTCTTGACTGTGTGTTGTGT 149 23 0 43.48 58.981 4.00 0.00 4.019 120 TAGCTTCTTGACTGTGTGTTGTGT 148 24 0 41.67 59.030 4.00 0.00 4.970 121 GTAGCTTCTTGACTGTGTGTTGTGT 147 25 0 44.00 59.835 4.00 0.00 5.165 122 TGTAGCTTCTTGACTGTGTGTTGTGT 146 26 0 42.31 62.367 4.00 0.00 8.367 123 ATGTAGCTTCTTGACTGTGTGTTGTGT 145 27 0 40.74 62.495 4.00 0.00 9.495 124 AGCTTCTTGACTGTGTGTTGTG 149 22 0 45.45 58.075 4.00 0.00 3.925 125 TAGCTTCTTGACTGTGTGTTGTG 148 23 0 43.48 58.160 4.00 0.00 4.840 126 GTAGCTTCTTGACTGTGTGTTGTG 147 24 0 45.83 59.030 4.00 0.00 4.970 127 TGTAGCTTCTTGACTGTGTGTTGTG 146 25 0 44.00 61.678 4.00 0.00 6.678 128 ATGTAGCTTCTTGACTGTGTGTTGTG 145 26 0 42.31 61.844 4.00 0.00 7.844 129 TGTAGCTTCTTGACTGTGTGTTGT 146 24 0 41.67 59.030 4.00 0.00 4.970 130 ATGTAGCTTCTTGACTGTGTGTTGT 145 25 0 40.00 59.333 4.00 0.00 5.667 131 GATGTAGCTTCTTGACTGTGTGTTGT 144 26 0 42.31 61.001 4.00 0.00 7.001 132 TGTAGCTTCTTGACTGTGTGTTG 146 23 0 43.48 58.160 4.00 0.00 4.840 133 ATGTAGCTTCTTGACTGTGTGTTG 145 24 0 41.67 58.520 4.00 0.00 5.480 134 GATGTAGCTTCTTGACTGTGTGTTG 144 25 0 44.00 60.272 4.00 0.00 5.272 135 GATGTAGCTTCTTGACTGTGTGTT 144 24 0 41.67 57.626 4.00 0.00 6.374 136 GGATGTAGCTTCTTGACTGTGTGTT 143 25 0 44.00 60.962 4.00 0.00 5.962 137 GGATGTAGCTTCTTGACTGTGTGT 143 24 0 45.83 59.748 4.00 0.00 4.252 138 TGGATGTAGCTTCTTGACTGTGTGT 142 25 0 44.00 62.322 4.00 0.00 7.322 139 ATGGATGTAGCTTCTTGACTGTGTGT 141 26 0 42.31 62.454 4.00 0.00 8.454 140 TATGGATGTAGCTTCTTGACTGTGTGT 140 27 0 40.74 62.383 4.00 0.00 9.383 141 GGATGTAGCTTCTTGACTGTGTG 143 23 0 47.83 58.925 4.00 0.00 4.075 142 TGGATGTAGCTTCTTGACTGTGTG 142 24 0 45.83 61.619 4.00 0.00 5.619 143 ATGGATGTAGCTTCTTGACTGTGTG 141 25 0 44.00 61.791 4.00 0.00 6.791 144 TATGGATGTAGCTTCTTGACTGTGTG 140 26 0 42.31 61.740 4.00 0.00 7.740 145 GTATGGATGTAGCTTCTTGACTGTGTG 139 27 0 44.44 62.383 4.00 0.00 9.383 146 TGGATGTAGCTTCTTGACTGTGT 142 23 0 43.48 58.925 4.00 1.00 4.075 147 ATGGATGTAGCTTCTTGACTGTGT 141 24 0 41.67 59.238 4.00 1.00 4.762 148 TATGGATGTAGCTTCTTGACTGTGT 140 25 0 40.00 59.274 4.00 1.00 5.726 149 GTATGGATGTAGCTTCTTGACTGTGT 139 26 0 42.31 60.018 4.00 1.00 6.018 150 TGTATGGATGTAGCTTCTTGACTGTGT 138 27 0 40.74 62.383 4.00 1.00 9.383 151 TGGATGTAGCTTCTTGACTGTG 142 22 0 45.45 58.036 4.00 1.00 3.964 152 ATGGATGTAGCTTCTTGACTGTG 141 23 0 43.48 58.408 4.00 1.00 4.592 153 TATGGATGTAGCTTCTTGACTGTG 140 24 0 41.67 58.476 4.00 1.00 5.524 154 GTATGGATGTAGCTTCTTGACTGTG 139 25 0 44.00 59.274 4.00 1.00 5.726 155 TGTATGGATGTAGCTTCTTGACTGTG 138 26 0 42.31 61.740 4.00 1.00 7.740 156 GTGTATGGATGTAGCTTCTTGACTGTG 137 27 0 44.44 62.383 4.00 1.00 9.383 157 TGTATGGATGTAGCTTCTTGACTGT 138 25 0 40.00 59.274 4.00 3.00 5.726 158 GTGTATGGATGTAGCTTCTTGACTGT 137 26 0 42.31 60.018 4.00 3.00 6.018 159 TGTGTATGGATGTAGCTTCTTGACTGT 136 27 0 40.74 62.383 4.00 3.00 9.383 160 TGTATGGATGTAGCTTCTTGACTG 138 24 0 41.67 58.476 4.00 1.00 5.524 161 GTGTATGGATGTAGCTTCTTGACTG 137 25 0 44.00 59.274 4.00 1.00 5.726 162 TGTGTATGGATGTAGCTTCTTGACTG 136 26 0 42.31 61.740 4.00 1.00 7.740 163 TTGTGTATGGATGTAGCTTCTTGACTG 135 27 0 40.74 62.778 4.00 1.00 9.778 164 TGTGTATGGATGTAGCTTCTTGACT 136 25 0 40.00 59.274 4.00 1.00 5.726 165 TTGTGTATGGATGTAGCTTCTTGACT 135 26 0 38.46 60.455 4.00 1.00 6.455 166 GTTGTGTATGGATGTAGCTTCTTGACT 134 27 0 40.74 61.126 4.00 1.00 8.126 167 TGTGTATGGATGTAGCTTCTTGAC 136 24 0 41.67 58.339 4.00 1.00 5.661 168 TTGTGTATGGATGTAGCTTCTTGAC 135 25 0 40.00 59.615 4.00 1.00 5.385 169 GTTGTGTATGGATGTAGCTTCTTGAC 134 26 0 42.31 60.343 4.00 1.00 6.343 170 TGTTGTGTATGGATGTAGCTTCTTGAC 133 27 0 40.74 62.685 4.00 1.00 9.685 171 TGTGTATGGATGTAGCTTCTTGA 136 23 0 39.13 57.467 4.00 2.00 5.533 172 TTGTGTATGGATGTAGCTTCTTGA 135 24 0 37.50 58.834 4.00 2.00 5.166 173 GTTGTGTATGGATGTAGCTTCTTGA 134 25 0 40.00 59.615 4.00 2.00 5.385 174 TGTTGTGTATGGATGTAGCTTCTTGA 133 26 0 38.46 62.056 4.00 2.00 8.056 175 GTGTTGTGTATGGATGTAGCTTCTTGA 132 27 0 40.74 62.685 4.00 2.00 9.685 176 TTGTGTATGGATGTAGCTTCTTG 135 23 0 39.13 57.036 4.00 2.00 5.964 177 GTTGTGTATGGATGTAGCTTCTTG 134 24 0 41.67 57.904 4.00 2.00 6.096 178 TGTTGTGTATGGATGTAGCTTCTTG 133 25 0 40.00 60.458 4.00 2.00 5.458 179 GTGTTGTGTATGGATGTAGCTTCTTG 132 26 0 42.31 61.157 4.00 2.00 7.157 180 TGTTGTGTATGGATGTAGCTTCTT 133 24 0 37.50 57.904 4.00 4.00 6.096 181 GTGTTGTGTATGGATGTAGCTTCTT 132 25 0 40.00 58.712 4.00 4.00 6.288 182 TGTGTTGTGTATGGATGTAGCTTCTT 131 26 0 38.46 61.157 4.00 4.00 7.157 183 GTGTGTTGTGTATGGATGTAGCTTCTT 130 27 0 40.74 61.811 4.00 4.00 8.811 184 GTGTTGTGTATGGATGTAGCTTCT 132 24 0 41.67 57.400 4.00 2.00 6.600 185 TGTGTTGTGTATGGATGTAGCTTCT 131 25 0 40.00 60.002 4.00 2.00 5.002 186 GTGTGTTGTGTATGGATGTAGCTTCT 130 26 0 42.31 60.727 4.00 2.00 6.727 187 TGTGTTGTGTATGGATGTAGCTTC 131 24 0 41.67 59.091 4.00 4.00 4.909 188 GTGTGTTGTGTATGGATGTAGCTTC 130 25 0 44.00 59.880 4.00 4.00 5.120 189 TGTGTGTTGTGTATGGATGTAGCTTC 129 26 0 42.31 62.368 4.00 4.00 8.368 190 ATGTGTGTTGTGTATGGATGTAGCTTC 128 27 0 40.74 62.494 4.00 4.00 9.494 191 TGTGTTGTGTATGGATGTAGCTT 131 23 0 39.13 57.257 4.00 2.00 5.743 192 GTGTGTTGTGTATGGATGTAGCTT 130 24 0 41.67 58.137 4.00 2.00 5.863 193 TGTGTGTTGTGTATGGATGTAGCTT 129 25 0 40.00 60.742 4.00 2.00 5.742 194 ATGTGTGTTGTGTATGGATGTAGCTT 128 26 0 38.46 60.948 4.00 2.00 6.948 195 TGTGTGTTGTGTATGGATGTAGCT 129 24 0 41.67 59.493 4.00 4.00 4.507 196 ATGTGTGTTGTGTATGGATGTAGCT 128 25 0 40.00 59.772 4.00 4.00 5.228 197 CATGTGTGTTGTGTATGGATGTAGCT 127 26 0 42.31 62.266 4.00 4.00 8.266 198 ACATGTGTGTTGTGTATGGATGTAGCT 126 27 0 40.74 62.902 6.00 4.00 9.902 199 TGTGTGTTGTGTATGGATGTAGC 129 23 0 43.48 58.499 2.00 2.00 4.501 200 ATGTGTGTTGTGTATGGATGTAGC 128 24 0 41.67 58.842 2.00 2.00 5.158 201 CATGTGTGTTGTGTATGGATGTAGC 127 25 0 44.00 61.472 4.00 2.00 6.472 202 ACATGTGTGTTGTGTATGGATGTAGC 126 26 0 42.31 62.163 6.00 2.00 8.163 203 TACATGTGTGTTGTGTATGGATGTAGC 125 27 0 40.74 62.100 6.00 2.00 9.100 204 CATGTGTGTTGTGTATGGATGTAG 127 24 0 41.67 57.896 4.00 0.00 6.104 205 ACATGTGTGTTGTGTATGGATGTAG 126 25 0 40.00 58.761 6.00 0.00 6.239 206 TACATGTGTGTTGTGTATGGATGTAG 125 26 0 38.46 58.815 6.00 0.00 7.185 207 ATACATGTGTGTTGTGTATGGATGTAG 124 27 0 37.04 59.113 6.00 0.00 7.887 208 ACATGTGTGTTGTGTATGGATGTA 126 24 0 37.50 57.747 6.00 2.00 6.253 209 TACATGTGTGTTGTGTATGGATGTA 125 25 0 36.00 57.841 6.00 2.00 7.159 210 ATACATGTGTGTTGTGTATGGATGTA 124 26 0 34.62 58.200 6.00 2.00 7.800 211 TATACATGTGTGTTGTGTATGGATGTA 123 27 0 33.33 58.271 6.00 2.00 8.729 212 ACATGTGTGTTGTGTATGGATGT 126 23 0 39.13 57.646 6.00 0.00 5.354 213 TACATGTGTGTTGTGTATGGATGT 125 24 0 37.50 57.747 6.00 0.00 6.253 214 ATACATGTGTGTTGTGTATGGATGT 124 25 0 36.00 58.124 6.00 0.00 6.876 215 TATACATGTGTGTTGTGTATGGATGT 123 26 0 34.62 58.200 6.00 0.00 7.800 216 ATATACATGTGTGTTGTGTATGGATGT 122 27 0 33.33 58.526 6.00 0.00 8.474 217 ATACATGTGTGTTGTGTATGGATG 124 24 0 37.50 57.241 6.00 0.00 6.759 218 TATACATGTGTGTTGTGTATGGATG 123 25 0 36.00 57.352 6.00 0.00 7.648 219 ATATACATGTGTGTTGTGTATGGATG 122 26 0 34.62 57.730 6.00 0.00 8.270 220 CATATACATGTGTGTTGTGTATGGATG 121 27 0 37.04 60.230 6.00 0.00 7.230 221 CATATACATGTGTGTTGTGTATGGAT 121 26 0 34.62 57.730 6.00 2.00 8.270 222 GCATATACATGTGTGTTGTGTATGGAT 120 27 0 37.04 61.010 6.00 2.00 8.010 223 CATATACATGTGTGTTGTGTATGGA 121 25 0 36.00 57.352 6.00 0.00 7.648 224 GCATATACATGTGTGTTGTGTATGGA 120 26 0 38.46 60.811 6.00 1.00 6.811 225 GCATATACATGTGTGTTGTGTATGG 120 25 0 40.00 59.136 6.00 3.00 5.864 226 TGCATATACATGTGTGTTGTGTATGG 119 26 0 38.46 61.654 6.00 3.00 7.654 227 GTGCATATACATGTGTGTTGTGTATGG 118 27 0 40.74 62.312 6.00 3.00 9.312 228 TGCATATACATGTGTGTTGTGTATG 119 25 0 36.00 58.378 6.00 5.00 6.622 229 GTGCATATACATGTGTGTTGTGTATG 118 26 0 38.46 59.190 6.00 5.00 6.810 230 TGTGCATATACATGTGTGTTGTGTATG 117 27 0 37.04 61.688 6.00 5.00 8.688 231 TGTGCATATACATGTGTGTTGTGTAT 117 26 0 34.62 59.190 6.00 3.00 6.810 232 TTGTGCATATACATGTGTGTTGTGTAT 116 27 0 33.33 60.389 6.00 3.00 7.389 233 TGTGCATATACATGTGTGTTGTGTA 117 25 0 36.00 58.882 6.00 2.00 6.118 234 TTGTGCATATACATGTGTGTTGTGTA 116 26 0 34.62 60.156 6.00 2.00 6.156 235 ATTGTGCATATACATGTGTGTTGTGTA 115 27 0 33.33 60.389 6.00 2.00 7.389 236 TGTGCATATACATGTGTGTTGTGT 117 24 0 37.50 58.828 6.00 0.00 5.172 237 TTGTGCATATACATGTGTGTTGTGT 116 25 0 36.00 60.151 6.00 0.00 5.151 238 ATTGTGCATATACATGTGTGTTGTGT 115 26 0 34.62 60.393 6.00 0.00 6.393 239 TATTGTGCATATACATGTGTGTTGTGT 114 27 0 33.33 60.389 6.00 0.00 7.389 240 TGTGCATATACATGTGTGTTGTG 117 23 0 39.13 57.923 6.00 2.00 5.077 241 TTGTGCATATACATGTGTGTTGTG 116 24 0 37.50 59.345 6.00 2.00 4.655 242 ATTGTGCATATACATGTGTGTTGTG 115 25 0 36.00 59.637 6.00 2.00 5.363 243 TATTGTGCATATACATGTGTGTTGTG 114 26 0 34.62 59.659 6.00 2.00 6.341 244 GTATTGTGCATATACATGTGTGTTGTG 113 27 0 37.04 60.389 6.00 2.00 7.389 245 TATTGTGCATATACATGTGTGTTGT 114 25 0 32.00 57.073 6.00 2.00 7.927 246 GTATTGTGCATATACATGTGTGTTGT 113 26 0 34.62 57.913 6.00 2.00 8.087 247 GGTATTGTGCATATACATGTGTGTTGT 112 27 0 37.04 61.034 6.00 2.00 8.034 248 GTATTGTGCATATACATGTGTGTTG 113 25 0 36.00 57.073 6.00 4.00 7.927 249 GGTATTGTGCATATACATGTGTGTTG 112 26 0 38.46 60.346 6.00 4.00 6.346 250 TGGTATTGTGCATATACATGTGTGTTG 111 27 0 37.04 62.716 6.00 4.00 9.716 251 GGTATTGTGCATATACATGTGTGTT 112 25 0 36.00 57.851 6.00 4.00 7.149 252 TGGTATTGTGCATATACATGTGTGTT 111 26 0 34.62 60.346 6.00 4.00 6.346 253 GTGGTATTGTGCATATACATGTGTGTT 110 27 0 37.04 61.034 6.00 4.00 8.034 254 TGGTATTGTGCATATACATGTGTGT 111 25 0 36.00 59.136 6.00 6.00 5.864 255 GTGGTATTGTGCATATACATGTGTGT 110 26 0 38.46 59.900 6.00 6.00 6.100 256 TGTGGTATTGTGCATATACATGTGTGT 109 27 0 37.04 62.312 6.00 6.00 9.312 257 TGGTATTGTGCATATACATGTGTG 111 24 0 37.50 58.315 6.00 4.00 5.685 258 GTGGTATTGTGCATATACATGTGTG 110 25 0 40.00 59.136 6.00 4.00 5.864 259 TGTGGTATTGTGCATATACATGTGTG 109 26 0 38.46 61.654 6.00 4.00 7.654 260 GTGTGGTATTGTGCATATACATGTGTG 108 27 0 40.74 62.312 6.00 4.00 9.312 261 TGTGGTATTGTGCATATACATGTGT 109 25 0 36.00 59.136 6.00 6.00 5.864 262 GTGTGGTATTGTGCATATACATGTGT 108 26 0 38.46 59.900 6.00 6.00 6.100 263 TGTGTGGTATTGTGCATATACATGTGT 107 27 0 37.04 62.312 6.00 6.00 9.312 264 TGTGGTATTGTGCATATACATGTG 109 24 0 37.50 58.315 6.00 4.00 5.685 265 GTGTGGTATTGTGCATATACATGTG 108 25 0 40.00 59.136 6.00 4.00 5.864 266 TGTGTGGTATTGTGCATATACATGTG 107 26 0 38.46 61.654 6.00 4.00 7.654 267 ATGTGTGGTATTGTGCATATACATGTG 106 27 0 37.04 61.814 6.00 4.00 8.814 268 TGTGTGGTATTGTGCATATACATGT 107 25 0 36.00 59.136 6.00 6.00 5.864 269 ATGTGTGGTATTGTGCATATACATGT 106 26 0 34.62 59.420 6.00 6.00 6.580 270 CATGTGTGGTATTGTGCATATACATGT 105 27 0 37.04 61.814 6.00 6.00 8.814 271 TGTGTGGTATTGTGCATATACATG 107 24 0 37.50 58.315 6.00 6.00 5.685 272 ATGTGTGGTATTGTGCATATACATG 106 25 0 36.00 58.650 6.00 6.00 6.350 273 CATGTGTGGTATTGTGCATATACATG 105 26 0 38.46 61.147 6.00 6.00 7.147 274 ACATGTGTGGTATTGTGCATATACATG 104 27 0 37.04 61.814 6.00 6.00 8.814 275 CATGTGTGGTATTGTGCATATACAT 105 25 0 36.00 58.650 6.00 4.00 6.350 276 ACATGTGTGGTATTGTGCATATACAT 104 26 0 34.62 59.420 6.00 4.00 6.580 277 AACATGTGTGGTATTGTGCATATACAT 103 27 0 33.33 60.562 6.00 4.00 7.562 278 CATGTGTGGTATTGTGCATATACA 105 24 0 37.50 58.315 6.00 6.00 5.685 279 ACATGTGTGGTATTGTGCATATACA 104 25 0 36.00 59.136 6.00 6.00 5.864 280 AACATGTGTGGTATTGTGCATATACA 103 26 0 34.62 60.346 6.00 6.00 6.346 281 AAACATGTGTGGTATTGTGCATATACA 102 27 0 33.33 61.445 6.00 6.00 8.445 282 AACATGTGTGGTATTGTGCATATAC 103 25 0 36.00 57.851 6.00 6.00 7.149 283 AAACATGTGTGGTATTGTGCATATAC 102 26 0 34.62 59.095 6.00 6.00 6.905 284 TAAACATGTGTGGTATTGTGCATATAC 101 27 0 33.33 59.133 6.00 6.00 7.867 285 AACATGTGTGGTATTGTGCATATA 103 24 0 33.33 57.002 6.00 4.00 6.998 286 AAACATGTGTGGTATTGTGCATATA 102 25 0 32.00 58.331 6.00 4.00 6.669 287 TAAACATGTGTGGTATTGTGCATATA 101 26 0 30.77 58.395 6.00 4.00 7.605 288 TTAAACATGTGTGGTATTGTGCATATA 100 27 0 29.63 59.560 6.00 4.00 7.440 289 AAACATGTGTGGTATTGTGCATAT 102 24 0 33.33 58.262 6.00 4.00 5.738 290 TAAACATGTGTGGTATTGTGCATAT 101 25 0 32.00 58.331 6.00 4.00 6.669 291 TTAAACATGTGTGGTATTGTGCATAT 100 26 0 30.77 59.536 6.00 4.00 6.464 292 GTTAAACATGTGTGGTATTGTGCATAT 99 27 0 33.33 60.227 6.00 4.00 7.227 293 AAACATGTGTGGTATTGTGCATA 102 23 0 34.78 57.903 6.00 4.00 5.097 294 TAAACATGTGTGGTATTGTGCATA 101 24 0 33.33 57.988 6.00 4.00 6.012 295 TTAAACATGTGTGGTATTGTGCATA 100 25 0 32.00 59.268 6.00 4.00 5.732 296 GTTAAACATGTGTGGTATTGTGCATA 99 26 0 34.62 60.001 6.00 4.00 6.001 297 TGTTAAACATGTGTGGTATTGTGCATA 98 27 0 33.33 62.329 7.00 4.00 9.329 298 AAACATGTGTGGTATTGTGCAT 102 22 0 36.36 57.811 6.00 2.00 4.189 299 TAAACATGTGTGGTATTGTGCAT 101 23 0 34.78 57.903 6.00 2.00 5.097 300 TTAAACATGTGTGGTATTGTGCAT 100 24 0 33.33 59.233 6.00 2.00 4.767 301 GTTAAACATGTGTGGTATTGTGCAT 99 25 0 36.00 59.991 6.00 2.00 5.009 302 TGTTAAACATGTGTGGTATTGTGCAT 98 26 0 34.62 62.397 7.00 2.00 8.397 303 AAACATGTGTGGTATTGTGCA 102 21 0 38.10 57.391 6.00 4.00 3.609 304 TAAACATGTGTGGTATTGTGCA 101 22 0 36.36 57.504 6.00 4.00 4.496 305 TTAAACATGTGTGGTATTGTGCA 100 23 0 34.78 58.925 6.00 4.00 4.075 306 GTTAAACATGTGTGGTATTGTGCA 99 24 0 37.50 59.733 6.00 4.00 4.267 307 TGTTAAACATGTGTGGTATTGTGCA 98 25 0 36.00 62.264 7.00 4.00 7.264 308 GTTAAACATGTGTGGTATTGTGC 99 23 0 39.13 57.060 6.00 2.00 5.940 309 TGTTAAACATGTGTGGTATTGTGC 98 24 0 37.50 59.733 7.00 2.00 4.267 310 CTGTTAAACATGTGTGGTATTGTGC 97 25 0 40.00 60.599 7.00 2.00 5.599 311 TCTGTTAAACATGTGTGGTATTGTGC 96 26 0 38.46 62.185 7.00 2.00 8.185 312 GTCTGTTAAACATGTGTGGTATTGTGC 95 27 0 40.74 62.806 7.00 2.00 9.806 313 CTGTTAAACATGTGTGGTATTGTG 97 24 0 37.50 57.140 7.00 0.00 6.860 314 TCTGTTAAACATGTGTGGTATTGTG 96 25 0 36.00 58.912 7.00 0.00 6.088 315 GTCTGTTAAACATGTGTGGTATTGTG 95 26 0 38.46 59.680 7.00 0.00 6.320 316 TGTCTGTTAAACATGTGTGGTATTGTG 94 27 0 37.04 62.089 7.00 0.00 9.089 317 GTCTGTTAAACATGTGTGGTATTGT 95 25 0 36.00 57.143 7.00 0.00 7.857 318 TGTCTGTTAAACATGTGTGGTATTGT 94 26 0 34.62 59.680 7.00 0.00 6.320 319 GTGTCTGTTAAACATGTGTGGTATTGT 93 27 0 37.04 60.396 7.00 0.00 7.396 320 TGTCTGTTAAACATGTGTGGTATTG 94 25 0 36.00 58.912 7.00 0.00 6.088 321 GTGTCTGTTAAACATGTGTGGTATTG 93 26 0 38.46 59.680 7.00 0.00 6.320 322 TGTGTCTGTTAAACATGTGTGGTATTG 92 27 0 37.04 62.089 7.00 0.00 9.089 323 GTGTCTGTTAAACATGTGTGGTATT 93 25 0 36.00 57.143 7.00 1.00 7.857 324 TGTGTCTGTTAAACATGTGTGGTATT 92 26 0 34.62 59.680 7.00 1.00 6.320 325 GTGTGTCTGTTAAACATGTGTGGTATT 91 27 0 37.04 60.396 7.00 1.00 7.396 326 TGTGTCTGTTAAACATGTGTGGTAT 92 25 0 36.00 58.424 7.00 2.00 6.576 327 GTGTGTCTGTTAAACATGTGTGGTAT 91 26 0 38.46 59.220 7.00 2.00 6.780 328 GGTGTGTCTGTTAAACATGTGTGGTAT 90 27 0 40.74 62.286 7.00 2.00 9.286 329 TGTGTCTGTTAAACATGTGTGGTA 92 24 0 37.50 58.069 7.00 2.00 5.931 330 GTGTGTCTGTTAAACATGTGTGGTA 91 25 0 40.00 58.919 7.00 2.00 6.081 331 GGTGTGTCTGTTAAACATGTGTGGTA 90 26 0 42.31 62.150 7.00 2.00 8.150 332 TGTGTCTGTTAAACATGTGTGGT 92 23 0 39.13 57.983 7.00 0.00 5.017 333 GTGTGTCTGTTAAACATGTGTGGT 91 24 0 41.67 58.868 7.00 0.00 5.132 334 GGTGTGTCTGTTAAACATGTGTGGT 90 25 0 44.00 62.217 7.00 0.00 7.217 335 TGTGTCTGTTAAACATGTGTGG 92 22 0 40.91 57.024 7.00 2.00 4.976 336 GTGTGTCTGTTAAACATGTGTGG 91 23 0 43.48 57.983 7.00 2.00 5.017 337 GGTGTGTCTGTTAAACATGTGTGG 90 24 0 45.83 61.509 7.00 2.00 5.509 338 GGTGTGTCTGTTAAACATGTGTG 90 23 0 43.48 57.983 7.00 4.00 5.017 339 GGGTGTGTCTGTTAAACATGTGTG 89 24 0 45.83 61.509 7.00 4.00 5.509 340 GGGTGTGTCTGTTAAACATGTGT 89 23 0 43.48 58.808 7.00 6.00 4.192 341 TGGGTGTGTCTGTTAAACATGTGT 88 24 0 41.67 61.509 7.00 6.00 5.509 342 GTGGGTGTGTCTGTTAAACATGTGT 87 25 0 44.00 62.217 7.00 6.00 7.217 343 GGGTGTGTCTGTTAAACATGTG 89 22 0 45.45 57.912 7.00 4.00 4.088 344 TGGGTGTGTCTGTTAAACATGTG 88 23 0 43.48 60.746 7.00 4.00 3.746 345 GTGGGTGTGTCTGTTAAACATGTG 87 24 0 45.83 61.509 7.00 4.00 5.509 346 TGGGTGTGTCTGTTAAACATGT 88 22 0 40.91 57.912 7.00 6.00 4.088 347 GTGGGTGTGTCTGTTAAACATGT 87 23 0 43.48 58.808 7.00 6.00 4.192 348 TGTGGGTGTGTCTGTTAAACATGT 86 24 0 41.67 61.509 7.00 6.00 5.509 349 TTGTGGGTGTGTCTGTTAAACATGT 85 25 0 40.00 62.648 7.00 6.00 7.648 350 GTGGGTGTGTCTGTTAAACATG 87 22 0 45.45 57.912 7.00 5.00 4.088 351 TGTGGGTGTGTCTGTTAAACATG 86 23 0 43.48 60.746 7.00 5.00 3.746 352 TTGTGGGTGTGTCTGTTAAACATG 85 24 0 41.67 61.967 7.00 5.00 5.967 353 CTTGTGGGTGTGTCTGTTAAACATG 84 25 0 44.00 62.749 7.00 5.00 7.749 354 TGTGGGTGTGTCTGTTAAACAT 86 22 0 40.91 57.912 7.00 5.00 4.088 355 TTGTGGGTGTGTCTGTTAAACAT 85 23 0 39.13 59.319 7.00 5.00 3.681 356 CTTGTGGGTGTGTCTGTTAAACAT 84 24 0 41.67 60.239 7.00 5.00 4.239 357 TCTTGTGGGTGTGTCTGTTAAACAT 83 25 0 40.00 61.898 7.00 5.00 6.898 358 GTCTTGTGGGTGTGTCTGTTAAACAT 82 26 0 42.31 62.553 7.00 5.00 8.553 359 TGTGGGTGTGTCTGTTAAACA 86 21 0 42.86 57.489 7.00 7.00 3.511 360 TTGTGGGTGTGTCTGTTAAACA 85 22 0 40.91 58.997 7.00 7.00 3.003 361 CTTGTGGGTGTGTCTGTTAAACA 84 23 0 43.48 59.981 7.00 7.00 3.019 362 TCTTGTGGGTGTGTCTGTTAAACA 83 24 0 41.67 61.733 7.00 7.00 5.733 363 GTCTTGTGGGTGTGTCTGTTAAACA 82 25 0 44.00 62.427 7.00 7.00 7.427 364 CTTGTGGGTGTGTCTGTTAAAC 84 22 0 45.45 57.176 5.00 5.00 4.824 365 TCTTGTGGGTGTGTCTGTTAAAC 83 23 0 43.48 59.063 5.00 5.00 3.937 366 GTCTTGTGGGTGTGTCTGTTAAAC 82 24 0 45.83 59.875 5.00 5.00 4.125 367 TGTCTTGTGGGTGTGTCTGTTAAAC 81 25 0 44.00 62.427 5.00 5.00 7.427 368 TCTTGTGGGTGTGTCTGTTAAA 83 22 0 40.91 58.187 4.00 3.00 3.813 369 GTCTTGTGGGTGTGTCTGTTAAA 82 23 0 43.48 59.063 4.00 3.00 3.937 370 TGTCTTGTGGGTGTGTCTGTTAAA 81 24 0 41.67 61.733 4.00 3.00 5.733 371 GTGTCTTGTGGGTGTGTCTGTTAAA 80 25 0 44.00 62.427 4.00 3.00 7.427 372 GTCTTGTGGGTGTGTCTGTTAA 82 22 0 45.45 57.635 4.00 4.00 4.365 373 TGTCTTGTGGGTGTGTCTGTTAA 81 23 0 43.48 60.493 4.00 4.00 3.493 374 GTGTCTTGTGGGTGTGTCTGTTAA 80 24 0 45.83 61.270 4.00 4.00 5.270 375 TGTCTTGTGGGTGTGTCTGTTA 81 22 0 45.45 59.108 2.00 2.00 2.892 376 GTGTCTTGTGGGTGTGTCTGTTA 80 23 0 47.83 59.984 2.00 2.00 3.016 377 TGTGTCTTGTGGGTGTGTCTGTTA 79 24 0 45.83 62.734 2.00 2.00 6.734 378 TGTCTTGTGGGTGTGTCTGTT 81 21 0 47.62 59.059 1.00 0.00 1.941 379 GTGTCTTGTGGGTGTGTCTGTT 80 22 0 50.00 59.972 1.00 0.00 2.028 380 TGTGTCTTGTGGGTGTGTCTGTT 79 23 0 47.83 62.831 1.00 0.00 5.831 381 TGTCTTGTGGGTGTGTCTGT 81 20 0 50.00 57.431 1.00 0.00 2.569 382 GTGTCTTGTGGGTGTGTCTGT 80 21 0 52.38 58.472 1.00 0.00 2.528 383 TGTGTCTTGTGGGTGTGTCTGT 79 22 0 50.00 61.549 1.00 0.00 3.549 384 GTGTCTTGTGGGTGTGTCTG 80 20 0 55.00 57.431 1.00 1.00 2.569 385 TGTGTCTTGTGGGTGTGTCTG 79 21 0 52.38 60.680 1.00 1.00 1.680 386 TGTGTCTTGTGGGTGTGTCT 79 20 0 50.00 57.431 1.00 0.00 2.569 387 TGTTGTGTATAAATGTTGTGTCTTCA 52 26 0 30.77 58.596 4.00 1.00 7.404 388 GTGTTGTGTATAAATGTTGTGTCTTCA 51 27 0 33.33 59.358 4.00 1.00 7.642 389 TGTGTTGTGTATAAATGTTGTGTCTTC 50 27 0 33.33 59.358 4.00 0.00 7.642 390 TGTGTTGTGTATAAATGTTGTGTCTT 50 26 0 30.77 57.700 4.00 0.00 8.300 391 ATGTGTTGTGTATAAATGTTGTGTCTT 49 27 0 29.63 58.041 4.00 0.00 8.959 392 CATATGTGTTGTGTATAAATGTTGTGT 46 27 0 29.63 57.112 6.00 0.00 9.888 393 ACATATGTGTTGTGTATAAATGTTGTG 45 27 0 29.63 57.112 8.00 0.00 9.888 394 CACATATGTGTTGTGTATAAATGTTGT 44 27 0 29.63 57.112 10.00 2.00 9.888 395 ACACATATGTGTTGTGTATAAATGTTG 43 27 0 29.63 57.112 12.00 0.00 9.888 396 CATGTGTGGCATTGTGTATACA 24 22 0 40.91 57.850 8.00 8.00 4.150 397 ACATGTGTGGCATTGTGTATACA 23 23 0 39.13 58.766 8.00 8.00 4.234 398 AACATGTGTGGCATTGTGTATACA 22 24 0 37.50 60.103 8.00 8.00 4.103 399 GAACATGTGTGGCATTGTGTATACA 21 25 0 40.00 61.810 8.00 8.00 6.810 400 AACATGTGTGGCATTGTGTATAC 22 23 0 39.13 57.373 6.00 6.00 5.627 401 GAACATGTGTGGCATTGTGTATAC 21 24 0 41.67 59.208 6.00 6.00 4.792 402 GAACATGTGTGGCATTGTGTATA 21 23 0 39.13 58.360 6.00 4.00 4.640 403 GAACATGTGTGGCATTGTGTAT 21 22 0 40.91 58.283 6.00 2.00 3.717 404 GAACATGTGTGGCATTGTGTA 21 21 0 42.86 57.880 6.00 2.00 3.120 405 GAACATGTGTGGCATTGTGT 21 20 0 45.00 57.775 6.00 0.00 2.225 primer3-2.3.6/test/primer1_list_last/SP01-02.txt.for0000664000077300007730000000217012231156243020720 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGTTTCACACTTACGGAAATGG 10 22 0 40.91 59.898 6.00 4.00 2.102 1 TTTCACACTTACGGAAATGGA 12 21 0 38.10 58.134 6.00 6.00 2.866 2 GTTTCACACTTACGGAAATGGA 11 22 0 40.91 58.997 6.00 6.00 3.003 3 GCTGTTTCACACTTACGGAAA 8 21 0 42.86 57.947 4.00 3.00 3.053 4 GCTGTTTCACACTTACGGAAAT 8 22 0 40.91 58.314 4.00 4.00 3.686 5 CTGTTTCACACTTACGGAAATGG 9 23 0 43.48 60.784 6.00 4.00 3.784 6 GTTTCACACTTACGGAAATGG 11 21 0 42.86 57.142 6.00 4.00 3.858 7 GCTGTTTCACACTTACGGAAATG 8 23 0 43.48 60.918 6.00 6.00 3.918 8 TGTTTCACACTTACGGAAATGGA 10 23 0 39.13 61.623 6.00 6.00 4.623 9 CTGTTTCACACTTACGGAAATG 9 22 0 40.91 57.367 6.00 6.00 4.633 10 CTTAGCGTGCGTGGGGTC 142 18 0 66.67 62.798 3.00 1.00 4.798 11 CTGTTTCACACTTACGGAAATGGA 9 24 0 41.67 62.411 6.00 6.00 6.411 primer3-2.3.6/test/primer1_list_last/SP04-07.txt.rev0000664000077300007730000012433112231156243020742 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TTCTTTCCGTGGCTCTCCTA 282 20 0 50.00 59.948 2.00 2.00 0.052 1 TCCCTTGGCCACATTCTTAG 142 20 0 50.00 60.066 6.00 3.00 0.066 2 CTTGGCCACATTCTTAGGGA 139 20 0 50.00 60.066 6.00 2.00 0.066 3 TGGCCACATTCTTAGGGAAC 137 20 0 50.00 59.933 6.00 0.00 0.067 4 GGCCACATTCTTAGGGAACA 136 20 0 50.00 59.933 4.00 0.00 0.067 5 CCTCATTTTCCCACAGCTGT 224 20 0 50.00 60.111 8.00 8.00 0.111 6 CTTCAGTGTGTACATGGCGG 115 20 0 55.00 60.175 6.00 1.00 0.175 7 ATTCTTTCCGTGGCTCTCCT 283 20 0 50.00 60.212 2.00 0.00 0.212 8 GCAGTTACACCACCCCATTC 299 20 0 55.00 60.240 3.00 0.00 0.240 9 TCTCCTAGCTGGGTTTGTGG 269 20 0 55.00 60.246 6.00 0.00 0.246 10 CCTAGCTGGGTTTGTGGCTA 266 20 0 55.00 60.262 6.00 2.00 0.262 11 TGTGGCTAGTCATGGAGGTG 254 20 0 55.00 59.701 6.00 0.00 0.299 12 AAGAGCCTCATTTTCCCACA 229 20 0 45.00 59.670 5.00 0.00 0.330 13 TACACCACCCCATTCTTTCC 294 20 0 50.00 59.647 2.00 0.00 0.353 14 GGGTTTGTGGCTAGTCATGG 259 20 0 55.00 60.375 6.00 2.00 0.375 15 TCTTTCCGTGGCTCTCCTAG 281 20 0 55.00 59.569 4.00 4.00 0.431 16 TGGGTTTGTGGCTAGTCATG 260 20 0 50.00 59.566 6.00 4.00 0.434 17 TTCCCTTGGCCACATTCTTA 143 20 0 45.00 60.439 6.00 2.00 0.439 18 TTGGCCACATTCTTAGGGAA 138 20 0 45.00 60.439 6.00 2.00 0.439 19 AGCTGGGTTTGTGGCTAGTC 263 20 0 55.00 59.357 4.00 4.00 0.643 20 CTAGTCATGGAGGTGCCCTG 249 20 0 60.00 60.668 4.00 2.00 0.668 21 GTGGCTAGTCATGGAGGTGC 253 20 0 60.00 60.688 6.00 2.00 0.688 22 ATTCCCTTGGCCACATTCTT 144 20 0 45.00 60.693 6.00 0.00 0.693 23 GCCCTGAAGAGCCTCATTTT 235 20 0 50.00 60.713 5.00 1.00 0.713 24 TGAAGAGCCTCATTTTCCCA 231 20 0 45.00 60.713 5.00 0.00 0.713 25 AGAGCCTCATTTTCCCACAG 228 20 0 50.00 59.284 5.00 1.00 0.716 26 GGAAGTGTGTGGTACACCCA 203 20 0 55.00 59.283 6.00 6.00 0.717 27 GTGGCTCTCCTAGCTGGGTT 274 20 0 60.00 60.786 6.00 2.00 0.786 28 GGGAAGGTCTTCAGTGCATT 161 20 0 50.00 59.141 6.00 4.00 0.859 29 AAGGTCTTCAGTGCATTCCC 158 20 0 50.00 59.141 4.00 2.00 0.859 30 GGTCTTCAGTGCATTCCCTT 156 20 0 50.00 59.141 4.00 2.00 0.859 31 TGGCTCTCCTAGCTGGGTT 273 19 0 57.89 59.958 6.00 2.00 1.042 32 TTCTTTCCGTGGCTCTCCT 282 19 0 52.63 59.935 2.00 0.00 1.065 33 TTCCGTGGCTCTCCTAGCT 278 19 0 57.89 60.104 4.00 4.00 1.104 34 CCTCATTTTCCCACAGCTGTA 224 21 0 47.62 60.118 8.00 6.00 1.118 35 TCCCACAGCTGTAGGAAGTG 216 20 0 55.00 58.879 12.00 2.00 1.121 36 AGGGAAGGTCTTCAGTGCATT 162 21 0 47.62 60.125 6.00 4.00 1.125 37 AAGGTCTTCAGTGCATTCCCT 158 21 0 47.62 60.125 4.00 4.00 1.125 38 AGGTCTTCAGTGCATTCCCTT 157 21 0 47.62 60.125 4.00 2.00 1.125 39 CCCTGAAGAGCCTCATTTTC 234 20 0 50.00 58.861 5.00 1.00 1.139 40 CCTGAAGAGCCTCATTTTCC 233 20 0 50.00 58.861 5.00 0.00 1.139 41 CTGAAGAGCCTCATTTTCCC 232 20 0 50.00 58.861 5.00 0.00 1.139 42 TTAGGGAAGGTCTTCAGTGCA 164 21 0 47.62 59.861 6.00 4.00 1.139 43 AGCCTCATTTTCCCACAGCT 226 20 0 50.00 61.153 4.00 4.00 1.153 44 TCTTCAGTGCATTCCCTTGG 154 20 0 50.00 61.176 4.00 2.00 1.176 45 TGCAGTTACACCACCCCAT 300 19 0 52.63 59.822 4.00 2.00 1.178 46 GGAAGTGTGTGGTACACCCAC 203 21 0 57.14 60.189 8.00 8.00 1.189 47 TGGCTAGTCATGGAGGTGC 252 19 0 57.89 59.803 6.00 2.00 1.197 48 GGCCACATTCTTAGGGAACAT 136 21 0 47.62 60.201 4.00 2.00 1.201 49 CCATTCTTTCCGTGGCTCT 285 19 0 52.63 60.203 3.00 0.00 1.203 50 ATGGAGGTGCCCTGAAGAG 243 19 0 57.89 60.207 4.00 2.00 1.207 51 AGCCTCATTTTCCCACAGC 226 19 0 52.63 60.211 3.00 2.00 1.211 52 GCCTCATTTTCCCACAGCT 225 19 0 52.63 60.211 4.00 4.00 1.211 53 ATTCTTTCCGTGGCTCTCCTA 283 21 0 47.62 60.214 2.00 2.00 1.214 54 TGCAGTTACACCACCCCATT 300 20 0 50.00 61.216 4.00 1.00 1.216 55 TCCCACAGCTGTAGGAAGTGT 216 21 0 52.38 59.777 12.00 1.00 1.223 56 GCTGGGTTTGTGGCTAGTCA 262 20 0 55.00 61.236 6.00 6.00 1.236 57 AGGGAAGGTCTTCAGTGCAT 162 20 0 50.00 58.744 6.00 2.00 1.256 58 AGGTCTTCAGTGCATTCCCT 157 20 0 50.00 58.744 4.00 4.00 1.256 59 CCTAGCTGGGTTTGTGGCT 266 19 0 57.89 60.262 6.00 0.00 1.262 60 TGGCTCTCCTAGCTGGGTTT 273 20 0 55.00 61.275 6.00 0.00 1.275 61 GGCTCTCCTAGCTGGGTTTG 272 20 0 60.00 61.275 6.00 2.00 1.275 62 TTTGTGGCTAGTCATGGAGG 256 20 0 50.00 58.722 6.00 0.00 1.278 63 GAAGAGCCTCATTTTCCCAC 230 20 0 50.00 58.717 5.00 0.00 1.283 64 TGTGTGGTACACCCACAATG 198 20 0 50.00 58.674 8.00 3.00 1.326 65 AGGAAGTGTGTGGTACACCCA 204 21 0 52.38 60.328 6.00 6.00 1.328 66 TAGTCATGGAGGTGCCCTG 248 19 0 57.89 59.641 4.00 2.00 1.359 67 GTGCCCTGAAGAGCCTCAT 237 19 0 57.89 60.365 5.00 5.00 1.365 68 GTGTGTGGTACACCCACAATG 199 21 0 52.38 59.632 8.00 3.00 1.368 69 TGTGTGGTACACCCACAATGT 198 21 0 47.62 59.632 8.00 5.00 1.368 70 ACACCACCCCATTCTTTCC 293 19 0 52.63 59.621 2.00 0.00 1.379 71 GGTTTGTGGCTAGTCATGGAG 258 21 0 52.38 59.607 6.00 0.00 1.393 72 GTTTGTGGCTAGTCATGGAGG 257 21 0 52.38 59.607 6.00 0.00 1.393 73 TTTGTGGCTAGTCATGGAGGT 256 21 0 47.62 59.607 6.00 0.00 1.393 74 CTTTCCGTGGCTCTCCTAGC 280 20 0 60.00 61.407 4.00 2.00 1.407 75 TTTCCGTGGCTCTCCTAGCT 279 20 0 55.00 61.407 4.00 4.00 1.407 76 GGTTTGTGGCTAGTCATGGA 258 20 0 50.00 58.570 6.00 2.00 1.430 77 TTCCCTTGGCCACATTCTT 143 19 0 47.37 60.445 6.00 0.00 1.445 78 GCTCTCCTAGCTGGGTTTGT 271 20 0 55.00 58.551 6.00 0.00 1.449 79 TTTCCGTGGCTCTCCTAGC 279 19 0 57.89 60.489 4.00 2.00 1.489 80 CTGGGTTTGTGGCTAGTCATG 261 21 0 52.38 60.556 6.00 4.00 1.556 81 TAGGGAAGGTCTTCAGTGCA 163 20 0 50.00 58.442 6.00 4.00 1.558 82 GTGGCTCTCCTAGCTGGGT 274 19 0 63.16 59.420 6.00 4.00 1.580 83 GCTAGTCATGGAGGTGCCCT 250 20 0 60.00 61.585 4.00 4.00 1.585 84 TAGCTGGGTTTGTGGCTAGTC 264 21 0 52.38 59.392 4.00 4.00 1.608 85 AAGAGCCTCATTTTCCCACAG 229 21 0 47.62 60.615 5.00 1.00 1.615 86 GCCCTGAAGAGCCTCATTT 235 19 0 52.63 59.376 5.00 3.00 1.624 87 TAGTCATGGAGGTGCCCTGA 248 20 0 55.00 61.630 4.00 4.00 1.630 88 GGCTAGTCATGGAGGTGCC 251 19 0 63.16 60.639 4.00 2.00 1.639 89 GCTAGTCATGGAGGTGCCC 250 19 0 63.16 60.639 4.00 2.00 1.639 90 ATTCCCTTGGCCACATTCT 144 19 0 47.37 59.351 6.00 1.00 1.649 91 GAAGTGTGTGGTACACCCACA 202 21 0 52.38 59.347 8.00 8.00 1.653 92 ATTCCCTTGGCCACATTCTTA 144 21 0 42.86 60.678 6.00 2.00 1.678 93 GTGCCCTGAAGAGCCTCATT 237 20 0 55.00 61.702 5.00 5.00 1.702 94 GTCTTCAGTGCATTCCCTTG 155 20 0 50.00 58.287 4.00 2.00 1.713 95 TCTTCAGTGTGTACATGGCG 116 20 0 50.00 58.276 6.00 2.00 1.724 96 CCCTTGGCCACATTCTTAGG 141 20 0 55.00 61.732 6.00 2.00 1.732 97 CCTTGGCCACATTCTTAGGG 140 20 0 55.00 61.732 6.00 0.00 1.732 98 CCCACAGCTGTAGGAAGTGTG 215 21 0 57.14 60.749 10.00 3.00 1.749 99 ATTCTTTCCGTGGCTCTCC 283 19 0 52.63 59.228 2.00 0.00 1.772 100 CCTCATTTTCCCACAGCTG 224 19 0 52.63 59.226 6.00 6.00 1.774 101 CTTCAGTGCATTCCCTTGG 153 19 0 52.63 59.226 4.00 2.00 1.774 102 TTGTGGCTAGTCATGGAGGT 255 20 0 50.00 58.158 6.00 0.00 1.842 103 TTCTTTCCGTGGCTCTCCTAG 282 21 0 52.38 60.870 4.00 4.00 1.870 104 TGCCCTGAAGAGCCTCATT 236 19 0 52.63 60.898 5.00 5.00 1.898 105 TTCAGTGTGTACATGGCGG 114 19 0 52.63 59.097 6.00 1.00 1.903 106 TCCCTTGGCCACATTCTTA 142 19 0 47.37 59.061 6.00 2.00 1.939 107 TTGGCCACATTCTTAGGGA 138 19 0 47.37 59.061 6.00 2.00 1.939 108 TGGCCACATTCTTAGGGAA 137 19 0 47.37 59.061 6.00 2.00 1.939 109 TTACACCACCCCATTCTTTCC 295 21 0 47.62 60.953 2.00 0.00 1.953 110 GGGAAGGTCTTCAGTGCATTC 161 21 0 52.38 61.014 6.00 2.00 2.014 111 GGAAGGTCTTCAGTGCATTCC 160 21 0 52.38 61.014 6.00 4.00 2.014 112 GAAGGTCTTCAGTGCATTCCC 159 21 0 52.38 61.014 6.00 2.00 2.014 113 CCATTCTTTCCGTGGCTCTC 285 20 0 55.00 62.053 3.00 0.00 2.053 114 CATTCTTTCCGTGGCTCTCC 284 20 0 55.00 62.053 2.00 0.00 2.053 115 AGGTGCCCTGAAGAGCCT 239 18 0 61.11 59.945 4.00 3.00 2.055 116 GAGCCTCATTTTCCCACAGC 227 20 0 55.00 62.069 5.00 2.00 2.069 117 GTGCCCTGAAGAGCCTCA 237 18 0 61.11 60.071 5.00 4.00 2.071 118 CTCCTAGCTGGGTTTGTGGC 268 20 0 60.00 62.090 6.00 2.00 2.090 119 TCCTAGCTGGGTTTGTGGCT 267 20 0 55.00 62.090 6.00 0.00 2.090 120 TGGAGGTGCCCTGAAGAG 242 18 0 61.11 59.903 4.00 2.00 2.097 121 TTGTGGCTAGTCATGGAGGTG 255 21 0 52.38 61.100 6.00 0.00 2.100 122 CACCCCATTCTTTCCGTG 289 18 0 55.56 59.886 3.00 0.00 2.114 123 ATTAGGGAAGGTCTTCAGTGCA 165 22 0 45.45 60.130 6.00 4.00 2.130 124 TTAGGGAAGGTCTTCAGTGCAT 164 22 0 45.45 60.130 6.00 2.00 2.130 125 TAGGGAAGGTCTTCAGTGCATT 163 22 0 45.45 60.130 6.00 4.00 2.130 126 CAGTTACACCACCCCATTCTTT 298 22 0 45.45 60.144 3.00 0.00 2.144 127 CCTAGCTGGGTTTGTGGCTAG 266 21 0 57.14 61.155 6.00 4.00 2.155 128 CATTCCCTTGGCCACATTCT 145 20 0 50.00 62.168 6.00 1.00 2.168 129 CTCTCCTAGCTGGGTTTGTGG 270 21 0 57.14 61.173 6.00 0.00 2.173 130 CAGTTACACCACCCCATTCTT 298 21 0 47.62 58.826 3.00 0.00 2.174 131 GCAGTTACACCACCCCATTCT 299 21 0 52.38 61.176 3.00 0.00 2.176 132 AAGTGTGTGGTACACCCACAA 201 21 0 47.62 58.817 8.00 6.00 2.183 133 TGCCCTGAAGAGCCTCATTT 236 20 0 50.00 62.185 5.00 3.00 2.185 134 TCCTAGCTGGGTTTGTGGC 267 19 0 57.89 61.192 6.00 2.00 2.192 135 TGTGGTACACCCACAATGTCTT 196 22 0 45.45 60.193 6.00 1.00 2.193 136 CATGGAGGTGCCCTGAAG 244 18 0 61.11 60.197 4.00 2.00 2.197 137 TAGGGAAGGTCTTCAGTGCAT 163 21 0 47.62 58.803 6.00 2.00 2.197 138 CCCACAGCTGTAGGAAGTGT 215 20 0 55.00 57.801 10.00 1.00 2.199 139 TTGGCCACATTCTTAGGGAAC 138 21 0 47.62 61.214 6.00 0.00 2.214 140 TGTGGTACACCCACAATGTCT 196 21 0 47.62 58.765 6.00 1.00 2.235 141 CATTCCCTTGGCCACATTC 145 19 0 52.63 61.265 6.00 0.00 2.265 142 CAGTGTGTACATGGCGGC 112 18 0 61.11 59.685 6.00 3.00 2.315 143 CTAGCTGGGTTTGTGGCTAGTC 265 22 0 54.55 60.315 5.00 4.00 2.315 144 GCTCTCCTAGCTGGGTTTGTG 271 21 0 57.14 61.316 6.00 0.00 2.316 145 CCGTGGCTCTCCTAGCTG 276 18 0 66.67 59.676 4.00 2.00 2.324 146 CGTGGCTCTCCTAGCTGG 275 18 0 66.67 59.676 4.00 4.00 2.324 147 TAGGAAGTGTGTGGTACACCCA 205 22 0 50.00 60.326 6.00 6.00 2.326 148 CTGGGTTTGTGGCTAGTCAT 261 20 0 50.00 57.671 6.00 4.00 2.329 149 GTGTGGTACACCCACAATGTCT 197 22 0 50.00 59.670 6.00 1.00 2.330 150 TTCCCTTGGCCACATTCTTAG 143 21 0 47.62 61.331 6.00 3.00 2.331 151 CTTGGCCACATTCTTAGGGAA 139 21 0 47.62 61.331 6.00 2.00 2.331 152 CTAGCTGGGTTTGTGGCTAG 265 20 0 55.00 57.665 5.00 4.00 2.335 153 ATCTTCAGTGTGTACATGGCG 117 21 0 47.62 58.664 6.00 2.00 2.336 154 AGTCATGGAGGTGCCCTG 247 18 0 61.11 59.612 4.00 2.00 2.388 155 GTGTGGTACACCCACAATGTC 197 21 0 52.38 58.609 6.00 3.00 2.391 156 TGTGGTACACCCACAATGTC 196 20 0 50.00 57.608 6.00 3.00 2.392 157 GTTTGTGGCTAGTCATGGAGGT 257 22 0 50.00 60.424 6.00 0.00 2.424 158 GCTGGGTTTGTGGCTAGTCAT 262 21 0 52.38 61.441 6.00 4.00 2.441 159 CTAGCTGGGTTTGTGGCTAGT 265 21 0 52.38 58.548 5.00 2.00 2.452 160 CTCTCCTAGCTGGGTTTGTG 270 20 0 55.00 57.547 6.00 0.00 2.453 161 TCTTTCCGTGGCTCTCCTA 281 19 0 52.63 58.528 2.00 2.00 2.472 162 GCCACATTCTTAGGGAACATC 135 21 0 47.62 58.517 3.00 1.00 2.483 163 TAGCTGGGTTTGTGGCTAGT 264 20 0 50.00 57.512 4.00 2.00 2.488 164 TGAAGAGCCTCATTTTCCCAC 231 21 0 47.62 61.500 5.00 0.00 2.500 165 GAAGAGCCTCATTTTCCCACA 230 21 0 47.62 61.500 5.00 0.00 2.500 166 GTGTGTGGTACACCCACAATGT 199 22 0 50.00 60.510 8.00 5.00 2.510 167 GCCACATTCTTAGGGAACATCT 135 22 0 45.45 59.487 3.00 2.00 2.513 168 TGCAGTTACACCACCCCA 300 18 0 55.56 59.486 4.00 0.00 2.514 169 GGCTCTCCTAGCTGGGTTT 272 19 0 57.89 58.486 6.00 0.00 2.514 170 ACCACCCCATTCTTTCCGT 291 19 0 52.63 61.532 2.00 0.00 2.532 171 TGCCCTGAAGAGCCTCAT 236 18 0 55.56 59.452 5.00 5.00 2.548 172 CTGAAGAGCCTCATTTTCCCA 232 21 0 47.62 61.618 5.00 0.00 2.618 173 CATTCTTTCCGTGGCTCTC 284 19 0 52.63 58.373 2.00 0.00 2.627 174 AGGGAAGGTCTTCAGTGCA 162 19 0 52.63 58.364 6.00 4.00 2.636 175 TTACACCACCCCATTCTTTC 295 20 0 45.00 57.364 2.00 0.00 2.636 176 CAGTTACACCACCCCATTCT 298 20 0 50.00 57.357 3.00 0.00 2.643 177 AGTGTGTGGTACACCCACAATG 200 22 0 50.00 60.644 8.00 4.00 2.644 178 GTCATGGAGGTGCCCTGA 246 18 0 61.11 60.651 4.00 4.00 2.651 179 TCCGTGGCTCTCCTAGCTG 277 19 0 63.16 61.652 4.00 2.00 2.652 180 AGGAAGTGTGTGGTACACCC 204 20 0 55.00 57.315 5.00 5.00 2.685 181 AGTCATGGAGGTGCCCTGA 247 19 0 57.89 61.688 4.00 4.00 2.688 182 GCTGGGTTTGTGGCTAGTC 262 19 0 57.89 58.301 4.00 4.00 2.699 183 CTCCTAGCTGGGTTTGTGG 268 19 0 57.89 58.300 6.00 0.00 2.700 184 CATTCCCTTGGCCACATT 145 18 0 50.00 59.296 6.00 1.00 2.704 185 GGAAGGTCTTCAGTGCATTC 160 20 0 50.00 57.288 6.00 2.00 2.712 186 GAAGGTCTTCAGTGCATTCC 159 20 0 50.00 57.288 6.00 3.00 2.712 187 ACCACCCCATTCTTTCCG 291 18 0 55.56 60.715 2.00 2.00 2.715 188 CCACCCCATTCTTTCCGT 290 18 0 55.56 60.715 2.00 0.00 2.715 189 ACCCCATTCTTTCCGTGG 288 18 0 55.56 60.715 3.00 2.00 2.715 190 GTTACACCACCCCATTCTTTC 296 21 0 47.62 58.280 3.00 0.00 2.720 191 GCAGTTACACCACCCCATT 299 19 0 52.63 58.273 3.00 1.00 2.727 192 AGTTACACCACCCCATTCTTTC 297 22 0 45.45 59.259 3.00 0.00 2.741 193 CCTAGCTGGGTTTGTGGC 266 18 0 61.11 59.241 6.00 2.00 2.759 194 GAAGTGTGTGGTACACCCACAA 202 22 0 50.00 60.765 8.00 6.00 2.765 195 AAGTGTGTGGTACACCCACA 201 20 0 50.00 57.229 8.00 8.00 2.771 196 AGTGTGTGGTACACCCACAA 200 20 0 50.00 57.229 8.00 6.00 2.771 197 TCAGTGTGTACATGGCGGC 113 19 0 57.89 61.775 6.00 3.00 2.775 198 TCAGTGCATTCCCTTGGC 151 18 0 55.56 60.784 4.00 2.00 2.784 199 AGAGCCTCATTTTCCCACA 228 19 0 47.37 58.216 5.00 0.00 2.784 200 GAGCCTCATTTTCCCACAG 227 19 0 52.63 58.216 5.00 1.00 2.784 201 CCACAGCTGTAGGAAGTGTGTG 214 22 0 54.55 60.796 8.00 1.00 2.796 202 CACAGCTGTAGGAAGTGTGTGG 213 22 0 54.55 60.796 8.00 0.00 2.796 203 GCCTCATTTTCCCACAGC 225 18 0 55.56 59.170 2.00 2.00 2.830 204 CCATTCTTTCCGTGGCTC 285 18 0 55.56 59.166 3.00 1.00 2.834 205 AAGTGTGTGGTACACCCACAAT 201 22 0 45.45 59.161 8.00 8.00 2.839 206 CCCTTGGCCACATTCTTAG 141 19 0 52.63 58.159 6.00 3.00 2.841 207 CCTTGGCCACATTCTTAGG 140 19 0 52.63 58.159 6.00 2.00 2.841 208 CTTGGCCACATTCTTAGGG 139 19 0 52.63 58.159 6.00 0.00 2.841 209 ATGGAGGTGCCCTGAAGA 243 18 0 55.56 59.129 4.00 0.00 2.871 210 GAGGTGCCCTGAAGAGCCT 240 19 0 63.16 61.885 4.00 3.00 2.885 211 AGGTGCCCTGAAGAGCCTC 239 19 0 63.16 61.885 5.00 5.00 2.885 212 TTCCGTGGCTCTCCTAGCTG 278 20 0 60.00 62.904 4.00 2.00 2.904 213 GGAGGTGCCCTGAAGAGC 241 18 0 66.67 60.924 4.00 4.00 2.924 214 GAGGTGCCCTGAAGAGCC 240 18 0 66.67 60.924 4.00 2.00 2.924 215 GGTGCCCTGAAGAGCCTC 238 18 0 66.67 60.924 5.00 5.00 2.924 216 TTCCGTGGCTCTCCTAGC 278 18 0 61.11 59.063 4.00 2.00 2.937 217 GGTCTTCAGTGCATTCCCTTG 156 21 0 52.38 61.957 4.00 2.00 2.957 218 GTCTTCAGTGCATTCCCTTGG 155 21 0 52.38 61.957 4.00 2.00 2.957 219 TTCCCTTGGCCACATTCT 143 18 0 50.00 59.009 6.00 1.00 2.991 220 TCCCTTGGCCACATTCTT 142 18 0 50.00 59.009 6.00 0.00 2.991 221 AGTCATGGAGGTGCCCTGAA 247 20 0 55.00 62.996 4.00 2.00 2.996 222 GTCATGGAGGTGCCCTGAAG 246 20 0 60.00 62.996 4.00 2.00 2.996 223 CCCATTCTTTCCGTGGCT 286 18 0 55.56 61.004 3.00 0.00 3.004 224 GTGGCTCTCCTAGCTGGGTTT 274 21 0 57.14 62.011 6.00 0.00 3.011 225 GGCTCTCCTAGCTGGGTTTGT 272 21 0 57.14 62.011 6.00 0.00 3.011 226 CCTCATTTTCCCACAGCTGTAG 224 22 0 50.00 61.023 8.00 8.00 3.023 227 TCCTAGCTGGGTTTGTGGCTA 267 21 0 52.38 62.023 6.00 2.00 3.023 228 ATTCTTTCCGTGGCTCTCCTAG 283 22 0 50.00 61.079 4.00 4.00 3.079 229 GTCATGGAGGTGCCCTGAA 246 19 0 57.89 62.091 4.00 2.00 3.091 230 TCTTCAGTGTGTACATGGCGG 116 21 0 52.38 62.092 6.00 1.00 3.092 231 TTCTTTCCGTGGCTCTCC 282 18 0 55.56 58.878 2.00 0.00 3.122 232 AGCTGGGTTTGTGGCTAGTCA 263 21 0 52.38 62.124 6.00 6.00 3.124 233 CCACAGCTGTAGGAAGTGTGT 214 21 0 52.38 57.875 8.00 1.00 3.125 234 ACAGCTGTAGGAAGTGTGTGG 212 21 0 52.38 57.875 8.00 0.00 3.125 235 CAGCTGTAGGAAGTGTGTGGT 211 21 0 52.38 57.875 6.00 0.00 3.125 236 TATTAGGGAAGGTCTTCAGTGCA 166 23 0 43.48 60.136 6.00 4.00 3.136 237 AGGAAGTGTGTGGTACACCCAC 204 22 0 54.55 61.149 8.00 8.00 3.149 238 TTCAGTGCATTCCCTTGGC 152 19 0 52.63 62.155 4.00 2.00 3.155 239 TGTGGTACACCCACAATGTCTTA 196 23 0 43.48 60.196 6.00 2.00 3.196 240 ACAGCTGTAGGAAGTGTGTGGT 212 22 0 50.00 58.799 8.00 0.00 3.201 241 GGGTTTGTGGCTAGTCATGGA 259 21 0 52.38 62.203 6.00 2.00 3.203 242 TGAAGAGCCTCATTTTCCC 231 19 0 47.37 57.791 5.00 0.00 3.209 243 GAAGAGCCTCATTTTCCCA 230 19 0 47.37 57.791 5.00 0.00 3.209 244 TCATGGAGGTGCCCTGAAG 245 19 0 57.89 62.218 4.00 2.00 3.218 245 CATGGAGGTGCCCTGAAGA 244 19 0 57.89 62.218 4.00 0.00 3.218 246 TCATGGAGGTGCCCTGAA 245 18 0 55.56 61.229 4.00 2.00 3.229 247 CTTTCCGTGGCTCTCCTAGCT 280 21 0 57.14 62.244 4.00 4.00 3.244 248 CCCTGAAGAGCCTCATTTTCC 234 21 0 52.38 62.304 5.00 0.00 3.304 249 CCTGAAGAGCCTCATTTTCCC 233 21 0 52.38 62.304 5.00 0.00 3.304 250 CACCACCCCATTCTTTCC 292 18 0 55.56 58.690 2.00 0.00 3.310 251 AGTGTGTGGTACACCCACAAT 200 21 0 47.62 57.686 8.00 8.00 3.314 252 CTAGTCATGGAGGTGCCCT 249 19 0 57.89 57.663 4.00 4.00 3.337 253 TCCGTGGCTCTCCTAGCT 277 18 0 61.11 58.643 4.00 4.00 3.357 254 AAGGTCTTCAGTGCATTCCCTT 158 22 0 45.45 61.364 4.00 3.00 3.364 255 GGGAAGGTCTTCAGTGCAT 161 19 0 52.63 57.630 6.00 2.00 3.370 256 AGGTCTTCAGTGCATTCCC 157 19 0 52.63 57.630 4.00 2.00 3.370 257 GGTCTTCAGTGCATTCCCT 156 19 0 52.63 57.630 4.00 4.00 3.370 258 CTTTCCGTGGCTCTCCTAG 280 19 0 57.89 57.628 4.00 4.00 3.372 259 ATTAGGGAAGGTCTTCAGTGCAT 165 23 0 43.48 60.373 6.00 2.00 3.373 260 GCTCTCCTAGCTGGGTTTG 271 19 0 57.89 57.626 6.00 2.00 3.374 261 GCCCTGAAGAGCCTCATTTTC 235 21 0 52.38 62.443 5.00 1.00 3.443 262 GGTACACCCACAATGTCTTAGC 193 22 0 50.00 58.513 5.00 2.00 3.487 263 CTCATTTTCCCACAGCTGTAG 223 21 0 47.62 57.499 8.00 8.00 3.501 264 ATTCCCTTGGCCACATTCTTAG 144 22 0 45.45 61.515 6.00 3.00 3.515 265 GTGGCTCTCCTAGCTGGG 274 18 0 66.67 58.482 6.00 6.00 3.518 266 TGGCTCTCCTAGCTGGGT 273 18 0 61.11 58.482 6.00 4.00 3.518 267 CTAGTCATGGAGGTGCCCTGA 249 21 0 57.14 62.530 4.00 4.00 3.530 268 GGTACACCCACAATGTCTTAGCT 193 23 0 47.83 59.468 5.00 4.00 3.532 269 CATCTTCAGTGTGTACATGGCG 118 22 0 50.00 61.537 6.00 2.00 3.537 270 CCCACAGCTGTAGGAAGTGTGT 215 22 0 54.55 61.541 10.00 1.00 3.541 271 TCTTTCCGTGGCTCTCCT 281 18 0 55.56 58.453 2.00 0.00 3.547 272 AGTTACACCACCCCATTCTTT 297 21 0 42.86 57.448 3.00 0.00 3.552 273 CCCACAATGTCTTAGCTTTTG 187 21 0 42.86 57.442 5.00 3.00 3.558 274 TAGGAAGTGTGTGGTACACCC 205 21 0 52.38 57.434 5.00 5.00 3.566 275 ATTAGGGAAGGTCTTCAGTGC 165 21 0 47.62 57.417 6.00 2.00 3.583 276 TGTGTGGTACACCCACAATGTC 198 22 0 50.00 61.591 8.00 3.00 3.591 277 AGCTGGGTTTGTGGCTAGT 263 19 0 52.63 57.400 4.00 2.00 3.600 278 GTAGGAAGTGTGTGGTACACCC 206 22 0 54.55 58.361 5.00 5.00 3.639 279 TGGCCACATTCTTAGGGAACA 137 21 0 47.62 62.668 6.00 0.00 3.668 280 GTTACACCACCCCATTCTTTCC 296 22 0 50.00 61.686 3.00 0.00 3.686 281 CACCCACAATGTCTTAGCTTTT 189 22 0 40.91 58.314 5.00 1.00 3.686 282 ACCCACAATGTCTTAGCTTTTG 188 22 0 40.91 58.314 5.00 3.00 3.686 283 CCCACAATGTCTTAGCTTTTGT 187 22 0 40.91 58.314 5.00 5.00 3.686 284 GCCACATTCTTAGGGAACATCTT 135 23 0 43.48 60.707 4.00 4.00 3.707 285 TCTTCAGTGCATTCCCTTG 154 19 0 47.37 57.292 4.00 2.00 3.708 286 ATTCCCTTGGCCACATTC 144 18 0 50.00 58.274 6.00 0.00 3.726 287 GTGGTACACCCACAATGTCTT 195 21 0 47.62 57.273 5.00 1.00 3.727 288 CTGGGTTTGTGGCTAGTCA 261 19 0 52.63 57.225 6.00 6.00 3.775 289 AGTGTGTACATGGCGGCG 111 18 0 61.11 61.777 6.00 3.00 3.777 290 TTGTGGCTAGTCATGGAGG 255 19 0 52.63 57.156 6.00 0.00 3.844 291 CCCATTCTTTCCGTGGCTC 286 19 0 57.89 62.863 3.00 1.00 3.863 292 CCTAGCTGGGTTTGTGGCTAGT 266 22 0 54.55 61.865 6.00 2.00 3.865 293 GCATTCCCTTGGCCACAT 146 18 0 55.56 61.869 6.00 2.00 3.869 294 ACACCCACAATGTCTTAGCTTTT 190 23 0 39.13 59.123 5.00 1.00 3.877 295 ACCCACAATGTCTTAGCTTTTGT 188 23 0 39.13 59.123 5.00 5.00 3.877 296 CATTCTTTCCGTGGCTCTCCT 284 21 0 52.38 62.881 2.00 0.00 3.881 297 AGGGAAGGTCTTCAGTGCATTC 162 22 0 50.00 61.884 6.00 2.00 3.884 298 GAAGGTCTTCAGTGCATTCCCT 159 22 0 50.00 61.884 6.00 4.00 3.884 299 TAGTCATGGAGGTGCCCTGAA 248 21 0 52.38 62.891 4.00 2.00 3.891 300 GTGCCCTGAAGAGCCTCATTT 237 21 0 52.38 62.898 5.00 3.00 3.898 301 AGAGCCTCATTTTCCCACAGC 228 21 0 52.38 62.898 5.00 2.00 3.898 302 GAGCCTCATTTTCCCACAGCT 227 21 0 52.38 62.898 5.00 4.00 3.898 303 TTCAGTGCATTCCCTTGG 152 18 0 50.00 58.095 4.00 2.00 3.905 304 CTCCTAGCTGGGTTTGTGGCT 268 21 0 57.14 62.907 6.00 0.00 3.907 305 CACCCACAATGTCTTAGCTTTTG 189 23 0 43.48 60.909 5.00 3.00 3.909 306 GGCCACATTCTTAGGGAACATC 136 22 0 50.00 61.913 4.00 1.00 3.913 307 AACATCTTCAGTGTGTACATGGC 120 23 0 43.48 59.046 6.00 2.00 3.954 308 GGCCACATTCTTAGGGAAC 136 19 0 52.63 57.030 4.00 0.00 3.970 309 CCACATTCTTAGGGAACATCTTC 134 23 0 43.48 59.014 6.00 6.00 3.986 310 GAAGTGTGTGGTACACCCACAAT 202 23 0 47.83 60.995 8.00 8.00 3.995 311 GTGTGGTACACCCACAATGTCTT 197 23 0 47.83 60.995 6.00 1.00 3.995 312 CAGCTGTAGGAAGTGTGTGGTA 211 22 0 50.00 57.969 6.00 2.00 4.031 313 CTTGGCCACATTCTTAGGGAAC 139 22 0 50.00 62.039 6.00 0.00 4.039 314 TAGCTGGGTTTGTGGCTAGTCA 264 22 0 50.00 62.057 6.00 6.00 4.057 315 TGTAGGAAGTGTGTGGTACACCC 207 23 0 52.17 61.117 5.00 5.00 4.117 316 GTAGGAAGTGTGTGGTACACCCA 206 23 0 52.17 61.117 6.00 6.00 4.117 317 TAGGAAGTGTGTGGTACACCCAC 205 23 0 52.17 61.117 8.00 8.00 4.117 318 GCCCTGAAGAGCCTCATT 235 18 0 55.56 57.879 5.00 5.00 4.121 319 ACAGCTGTAGGAAGTGTGTGGTA 212 23 0 47.83 58.855 8.00 2.00 4.145 320 CAGCTGTAGGAAGTGTGTGGTAC 211 23 0 52.17 58.855 6.00 4.00 4.145 321 AGCTGTAGGAAGTGTGTGGTACA 210 23 0 47.83 58.855 4.00 3.00 4.145 322 GCTGTAGGAAGTGTGTGGTACA 209 22 0 50.00 57.817 4.00 3.00 4.183 323 TGGTACACCCACAATGTCTTAGC 194 23 0 47.83 61.184 5.00 2.00 4.184 324 ACACCCACAATGTCTTAGCTTT 190 22 0 40.91 57.804 5.00 3.00 4.196 325 ATCTTCAGTGTGTACATGGCGG 117 22 0 50.00 62.253 6.00 1.00 4.253 326 AGCTGGGTTTGTGGCTAGTCAT 263 22 0 50.00 62.275 6.00 4.00 4.275 327 GCTGTAGGAAGTGTGTGGTACAC 209 23 0 52.17 58.715 5.00 5.00 4.285 328 TTCTTAGGGAACATCTTCAGTGTG 129 24 0 41.67 59.690 6.00 2.00 4.310 329 ACAGCTGTAGGAAGTGTGTGGTAC 212 24 0 50.00 59.674 8.00 4.00 4.326 330 AGCTGTAGGAAGTGTGTGGTACAC 210 24 0 50.00 59.674 5.00 5.00 4.326 331 TTAGGGAAGGTCTTCAGTGCATT 164 23 0 43.48 61.327 6.00 4.00 4.327 332 CTGAAGAGCCTCATTTTCCCAC 232 22 0 50.00 62.336 5.00 0.00 4.336 333 GAAGAGCCTCATTTTCCCACAG 230 22 0 50.00 62.336 5.00 1.00 4.336 334 TTTGTGGCTAGTCATGGAGGTG 256 22 0 50.00 62.349 6.00 0.00 4.349 335 AGGGAACATCTTCAGTGTGTACA 124 23 0 43.48 58.639 6.00 6.00 4.361 336 GCAGTTACACCACCCCATTCTT 299 22 0 50.00 62.366 3.00 0.00 4.366 337 TATTAGGGAAGGTCTTCAGTGCAT 166 24 0 41.67 60.370 6.00 2.00 4.370 338 ACATCTTCAGTGTGTACATGGC 119 22 0 45.45 57.601 6.00 2.00 4.399 339 GGGAACATCTTCAGTGTGTACA 123 22 0 45.45 57.574 6.00 6.00 4.426 340 CTGTAGGAAGTGTGTGGTACACC 208 23 0 52.17 58.570 5.00 4.00 4.430 341 AGTGTGTGGTACACCCACAATGT 200 23 0 47.83 61.441 8.00 5.00 4.441 342 TGGTACACCCACAATGTCTTAG 194 22 0 45.45 57.543 5.00 3.00 4.457 343 TATTAGGGAAGGTCTTCAGTGC 166 22 0 45.45 57.520 6.00 2.00 4.480 344 TGGCCACATTCTTAGGGA 137 18 0 50.00 57.514 6.00 2.00 4.486 345 TGTAGGAAGTGTGTGGTACACC 207 22 0 50.00 57.509 5.00 4.00 4.491 346 GTGCATTCCCTTGGCCAC 148 18 0 61.11 62.504 6.00 4.00 4.504 347 GTGGTACACCCACAATGTCTTAG 195 23 0 47.83 58.433 5.00 3.00 4.567 348 TCAGTGTGTACATGGCGG 113 18 0 55.56 57.425 6.00 1.00 4.575 349 TCCCACAGCTGTAGGAAGTGTG 216 22 0 54.55 62.576 12.00 3.00 4.576 350 CACAGCTGTAGGAAGTGTGTGGT 213 23 0 52.17 61.580 8.00 0.00 4.580 351 GTGGTACACCCACAATGTCTTA 195 22 0 45.45 57.389 5.00 2.00 4.611 352 CCCACAATGTCTTAGCTTTTGTA 187 23 0 39.13 58.382 5.00 3.00 4.618 353 TCTTAGGGAACATCTTCAGTGTG 128 23 0 43.48 58.382 6.00 2.00 4.618 354 CAGTGCATTCCCTTGGCC 150 18 0 61.11 62.628 4.00 4.00 4.628 355 AGTGCATTCCCTTGGCCA 149 18 0 55.56 62.628 6.00 6.00 4.628 356 GTATTAGGGAAGGTCTTCAGTGC 167 23 0 47.83 58.358 6.00 2.00 4.642 357 GGTACACCCACAATGTCTTAGCTT 193 24 0 45.83 60.673 5.00 2.00 4.673 358 GGGAACATCTTCAGTGTGTACATG 123 24 0 45.83 60.700 6.00 5.00 4.700 359 GGAACATCTTCAGTGTGTACATGG 122 24 0 45.83 60.700 6.00 3.00 4.700 360 CACATTCTTAGGGAACATCTTCA 133 23 0 39.13 58.251 6.00 4.00 4.749 361 CCACATTCTTAGGGAACATCTT 134 22 0 40.91 57.236 4.00 4.00 4.764 362 AGGTCTTCAGTGCATTCCCTTG 157 22 0 50.00 62.786 4.00 2.00 4.786 363 TCTTAGGGAACATCTTCAGTGTGT 128 24 0 41.67 59.212 6.00 2.00 4.788 364 CATTCTTTCCGTGGCTCTCCTA 284 22 0 50.00 62.788 2.00 2.00 4.788 365 TGGCCACATTCTTAGGGAACAT 137 22 0 45.45 62.791 6.00 2.00 4.791 366 CACATTCTTAGGGAACATCTTCAG 133 24 0 41.67 59.196 6.00 2.00 4.804 367 CATTCTTAGGGAACATCTTCAGTG 131 24 0 41.67 59.196 6.00 3.00 4.804 368 CTCCTAGCTGGGTTTGTGGCTA 268 22 0 54.55 62.814 6.00 2.00 4.814 369 TCCTAGCTGGGTTTGTGGCTAG 267 22 0 54.55 62.814 6.00 4.00 4.814 370 TAGGGAAGGTCTTCAGTGCATTC 163 23 0 47.83 61.828 6.00 2.00 4.828 371 CAGTTACACCACCCCATTCTTTC 298 23 0 47.83 61.831 3.00 0.00 4.831 372 TACACCCACAATGTCTTAGCTTTT 191 24 0 37.50 59.162 5.00 1.00 4.838 373 ACCCACAATGTCTTAGCTTTTGTA 188 24 0 37.50 59.162 5.00 3.00 4.838 374 GAACATCTTCAGTGTGTACATGGC 121 24 0 45.83 60.841 6.00 2.00 4.841 375 GGGAAGGTCTTCAGTGCA 161 18 0 55.56 57.158 6.00 4.00 4.842 376 TCCTAGCTGGGTTTGTGG 267 18 0 55.56 57.142 6.00 0.00 4.858 377 TGTATTAGGGAAGGTCTTCAGTGC 168 24 0 45.83 60.860 6.00 2.00 4.860 378 GTATTAGGGAAGGTCTTCAGTGCA 167 24 0 45.83 60.860 6.00 4.00 4.860 379 AAGTGTGTGGTACACCCACAATG 201 23 0 47.83 61.918 8.00 6.00 4.918 380 GAGCCTCATTTTCCCACA 227 18 0 50.00 57.033 5.00 0.00 4.967 381 GTGTGGTACACCCACAATGTCTTA 197 24 0 45.83 60.969 6.00 2.00 4.969 382 CCCTTGGCCACATTCTTA 141 18 0 50.00 57.022 6.00 2.00 4.978 383 TTGGCCACATTCTTAGGG 138 18 0 50.00 57.022 6.00 0.00 4.978 384 GGGAACATCTTCAGTGTGTACAT 123 23 0 43.48 57.976 6.00 4.00 5.024 385 TTAGGGAACATCTTCAGTGTGTACA 126 25 0 40.00 59.975 6.00 6.00 5.025 386 AGGGAACATCTTCAGTGTGTACAT 124 24 0 41.67 58.969 6.00 4.00 5.031 387 CACATTCTTAGGGAACATCTTCAGT 133 25 0 40.00 59.947 6.00 4.00 5.053 388 ACATTCTTAGGGAACATCTTCAGTG 132 25 0 40.00 59.947 6.00 3.00 5.053 389 CATTCTTAGGGAACATCTTCAGTGT 131 25 0 40.00 59.947 6.00 4.00 5.053 390 ATTCTTAGGGAACATCTTCAGTGTG 130 25 0 40.00 59.947 6.00 2.00 5.053 391 TTTGTATTAGGGAAGGTCTTCAGTG 170 25 0 40.00 59.946 6.00 3.00 5.054 392 TGTGGTACACCCACAATGTCTTAG 196 24 0 45.83 61.086 6.00 3.00 5.086 393 TACACCCACAATGTCTTAGCTTT 191 23 0 39.13 57.892 5.00 3.00 5.108 394 GTACACCCACAATGTCTTAGCTTTT 192 25 0 40.00 59.890 5.00 1.00 5.110 395 CCCACAATGTCTTAGCTTTTGTATT 187 25 0 36.00 59.858 5.00 1.00 5.142 396 GCTTTTGTATTAGGGAAGGTCTTC 173 24 0 41.67 58.814 6.00 6.00 5.186 397 TAGCTGGGTTTGTGGCTAGTCAT 264 23 0 47.83 62.206 6.00 4.00 5.206 398 TTGTATTAGGGAAGGTCTTCAGTG 169 24 0 41.67 58.759 6.00 3.00 5.241 399 ACATCTTCAGTGTGTACATGGCG 119 23 0 47.83 62.286 6.00 2.00 5.286 400 GTACACCCACAATGTCTTAGCTTT 192 24 0 41.67 58.699 5.00 3.00 5.301 401 TAGGGAACATCTTCAGTGTGTACA 125 24 0 41.67 58.699 6.00 6.00 5.301 402 CCCACAATGTCTTAGCTTTTGTAT 187 24 0 37.50 58.698 5.00 2.00 5.302 403 GTGTGTGGTACACCCACAATGTC 199 23 0 52.17 62.364 8.00 3.00 5.364 404 AGCTTTTGTATTAGGGAAGGTCTTC 174 25 0 40.00 59.629 6.00 6.00 5.371 405 TTCTTAGGGAACATCTTCAGTGTGT 129 25 0 40.00 60.434 6.00 2.00 5.434 406 AGTTACACCACCCCATTCTTTCC 297 23 0 47.83 62.469 3.00 0.00 5.469 407 TGTGTGGTACACCCACAATGTCT 198 23 0 47.83 62.479 8.00 1.00 5.479 408 ATTAGGGAAGGTCTTCAGTGCATT 165 24 0 41.67 61.502 6.00 4.00 5.502 409 CACAGCTGTAGGAAGTGTGTGGTA 213 24 0 50.00 61.532 8.00 2.00 5.532 410 CAGCTGTAGGAAGTGTGTGGTACA 211 24 0 50.00 61.532 6.00 3.00 5.532 411 CCACATTCTTAGGGAACATCTTCA 134 24 0 41.67 61.537 6.00 4.00 5.537 412 TGTATTAGGGAAGGTCTTCAGTG 168 23 0 43.48 57.450 6.00 3.00 5.550 413 ACCCACAATGTCTTAGCTTTTGTAT 188 25 0 36.00 59.432 5.00 2.00 5.568 414 CTTAGGGAACATCTTCAGTGTGT 127 23 0 43.48 57.416 6.00 2.00 5.584 415 ACACCCACAATGTCTTAGCTTTTG 190 24 0 41.67 61.607 5.00 3.00 5.607 416 CACCCACAATGTCTTAGCTTTTGT 189 24 0 41.67 61.607 5.00 5.00 5.607 417 GTACACCCACAATGTCTTAGCTT 192 23 0 43.48 57.388 5.00 2.00 5.612 418 GGCCACATTCTTAGGGAACATCT 136 23 0 47.83 62.687 4.00 2.00 5.687 419 TCTTAGGGAACATCTTCAGTGTGTA 128 25 0 40.00 59.249 6.00 2.00 5.751 420 GCTTTTGTATTAGGGAAGGTCTT 173 23 0 39.13 57.202 4.00 4.00 5.798 421 CTAGCTGGGTTTGTGGCTAGTCA 265 23 0 52.17 62.841 6.00 6.00 5.841 422 GTAGGAAGTGTGTGGTACACCCAC 206 24 0 54.17 61.849 8.00 8.00 5.849 423 GGAACATCTTCAGTGTGTACATG 122 23 0 43.48 57.126 6.00 5.00 5.874 424 GAACATCTTCAGTGTGTACATGG 121 23 0 43.48 57.126 6.00 3.00 5.874 425 GTGGTACACCCACAATGTCTTAGC 195 24 0 50.00 61.894 5.00 2.00 5.894 426 AGCTTTTGTATTAGGGAAGGTCTT 174 24 0 37.50 58.100 4.00 4.00 5.900 427 TTTTGTATTAGGGAAGGTCTTCA 171 23 0 34.78 57.052 6.00 4.00 5.948 428 TTCTTAGGGAACATCTTCAGTGT 129 23 0 39.13 57.049 6.00 4.00 5.951 429 CTGTAGGAAGTGTGTGGTACACCC 208 24 0 54.17 61.959 5.00 5.00 5.959 430 TAGGGAACATCTTCAGTGTGTACAT 125 25 0 40.00 59.015 6.00 4.00 5.985 431 TGGTACACCCACAATGTCTTAGCT 194 24 0 45.83 62.000 5.00 4.00 6.000 432 CTTTTGTATTAGGGAAGGTCTTCA 172 24 0 37.50 57.993 6.00 4.00 6.007 433 TTTTGTATTAGGGAAGGTCTTCAG 171 24 0 37.50 57.993 6.00 2.00 6.007 434 TCTTAGGGAACATCTTCAGTGTGTAC 128 26 0 42.31 59.993 6.00 4.00 6.007 435 GTATTAGGGAAGGTCTTCAGTGCAT 167 25 0 44.00 61.054 6.00 2.00 6.054 436 GCTGTAGGAAGTGTGTGGTACACC 209 24 0 54.17 62.095 5.00 4.00 6.095 437 GCTTTTGTATTAGGGAAGGTCTTCA 173 25 0 40.00 61.112 6.00 4.00 6.112 438 CCCACAATGTCTTAGCTTTTGTATTA 187 26 0 34.62 59.872 5.00 3.00 6.128 439 CTTTTGTATTAGGGAAGGTCTTCAG 172 25 0 40.00 58.861 6.00 2.00 6.139 440 TTAGGGAACATCTTCAGTGTGTACAT 126 26 0 38.46 60.216 6.00 4.00 6.216 441 TTTTGTATTAGGGAAGGTCTTCAGT 171 25 0 36.00 58.743 6.00 4.00 6.257 442 GCCACATTCTTAGGGAACATCTTC 135 24 0 45.83 62.298 6.00 6.00 6.298 443 TCTTAGCTTTTGTATTAGGGAAGGTC 178 26 0 38.46 59.649 4.00 1.00 6.351 444 TAGCTTTTGTATTAGGGAAGGTCTTC 175 26 0 38.46 59.649 6.00 6.00 6.351 445 TGTCTTAGCTTTTGTATTAGGGAAGG 180 26 0 38.46 60.381 4.00 1.00 6.381 446 TTCTTAGGGAACATCTTCAGTGTGTA 129 26 0 38.46 60.429 6.00 2.00 6.429 447 CTTTTGTATTAGGGAAGGTCTTCAGT 172 26 0 38.46 59.556 6.00 4.00 6.444 448 TATTAGGGAAGGTCTTCAGTGCATT 166 25 0 40.00 61.463 6.00 4.00 6.463 449 TTTGTATTAGGGAAGGTCTTCAGT 170 24 0 37.50 57.525 6.00 4.00 6.475 450 CTTAGGGAACATCTTCAGTGTGTA 127 24 0 41.67 57.518 6.00 2.00 6.482 451 ACCCACAATGTCTTAGCTTTTGTATT 188 26 0 34.62 60.524 5.00 1.00 6.524 452 AGGGAACATCTTCAGTGTGTACATG 124 25 0 44.00 61.548 6.00 5.00 6.548 453 ACATTCTTAGGGAACATCTTCAGT 132 24 0 37.50 57.442 6.00 4.00 6.558 454 ATTCTTAGGGAACATCTTCAGTGT 130 24 0 37.50 57.442 6.00 4.00 6.558 455 TACACCCACAATGTCTTAGCTTTTG 191 25 0 40.00 61.563 5.00 3.00 6.563 456 CACCCACAATGTCTTAGCTTTTGTA 189 25 0 40.00 61.563 5.00 3.00 6.563 457 TCTTAGCTTTTGTATTAGGGAAGG 178 24 0 37.50 57.427 4.00 1.00 6.573 458 TTAGGGAACATCTTCAGTGTGTAC 126 24 0 41.67 57.376 6.00 4.00 6.624 459 AAGTGTGTGGTACACCCACAATGT 201 24 0 45.83 62.627 8.00 6.00 6.627 460 ACATTCTTAGGGAACATCTTCAGTGT 132 26 0 38.46 60.648 6.00 4.00 6.648 461 ATTCTTAGGGAACATCTTCAGTGTGT 130 26 0 38.46 60.648 6.00 2.00 6.648 462 CTTAGGGAACATCTTCAGTGTGTAC 127 25 0 44.00 58.344 6.00 4.00 6.656 463 TTAGCTTTTGTATTAGGGAAGGTC 176 24 0 37.50 57.300 4.00 1.00 6.700 464 TTAGCTTTTGTATTAGGGAAGGTCTT 176 26 0 34.62 59.264 4.00 4.00 6.736 465 GGTACACCCACAATGTCTTAGCTTT 193 25 0 44.00 61.766 5.00 3.00 6.766 466 CAATGTCTTAGCTTTTGTATTAGGGA 183 26 0 34.62 59.231 4.00 0.00 6.769 467 GTGTGGTACACCCACAATGTCTTAG 197 25 0 48.00 61.794 6.00 3.00 6.794 468 CTTAGGGAACATCTTCAGTGTGTACA 127 26 0 42.31 60.811 6.00 6.00 6.811 469 GTCTTAGCTTTTGTATTAGGGAAGG 179 25 0 40.00 58.167 4.00 1.00 6.833 470 TCTTAGCTTTTGTATTAGGGAAGGT 178 25 0 36.00 58.167 4.00 3.00 6.833 471 CTTAGCTTTTGTATTAGGGAAGGTC 177 25 0 40.00 58.167 4.00 1.00 6.833 472 TTAGCTTTTGTATTAGGGAAGGTCT 176 25 0 36.00 58.167 4.00 2.00 6.833 473 TAGCTTTTGTATTAGGGAAGGTCTT 175 25 0 36.00 58.167 4.00 4.00 6.833 474 TTAGGGAAGGTCTTCAGTGCATTC 164 24 0 45.83 62.910 6.00 2.00 6.910 475 TTGTATTAGGGAAGGTCTTCAGTGC 169 25 0 44.00 61.945 6.00 2.00 6.945 476 CTAGCTGGGTTTGTGGCTAGTCAT 265 24 0 50.00 62.946 6.00 4.00 6.946 477 CTTAGCTTTTGTATTAGGGAAGGTCTT 177 27 0 37.04 59.997 4.00 4.00 7.003 478 TTTTGTATTAGGGAAGGTCTTCAGTG 171 26 0 38.46 61.028 6.00 3.00 7.028 479 CTTAGCTTTTGTATTAGGGAAGGTCT 177 26 0 38.46 58.971 4.00 2.00 7.029 480 ACAATGTCTTAGCTTTTGTATTAGGGA 184 27 0 33.33 59.891 5.00 0.00 7.109 481 GTCTTAGCTTTTGTATTAGGGAAGGT 179 26 0 38.46 58.862 4.00 3.00 7.138 482 CACAGCTGTAGGAAGTGTGTGGTAC 213 25 0 52.00 62.233 8.00 4.00 7.233 483 ACAGCTGTAGGAAGTGTGTGGTACA 212 25 0 48.00 62.233 8.00 3.00 7.233 484 CAGCTGTAGGAAGTGTGTGGTACAC 211 25 0 52.00 62.233 6.00 5.00 7.233 485 ACACCCACAATGTCTTAGCTTTTGT 190 25 0 40.00 62.258 5.00 5.00 7.258 486 CAATGTCTTAGCTTTTGTATTAGGGAA 183 27 0 33.33 60.279 4.00 1.00 7.279 487 GTCTTAGCTTTTGTATTAGGGAAGGTC 179 27 0 40.74 60.283 4.00 1.00 7.283 488 CAATGTCTTAGCTTTTGTATTAGGG 183 25 0 36.00 57.709 4.00 0.00 7.291 489 CCACATTCTTAGGGAACATCTTCAG 134 25 0 44.00 62.299 6.00 2.00 7.299 490 TCTTAGCTTTTGTATTAGGGAAGGTCT 178 27 0 37.04 60.382 4.00 2.00 7.382 491 TAGGGAACATCTTCAGTGTGTACATG 125 26 0 42.31 61.505 6.00 5.00 7.505 492 TGTCTTAGCTTTTGTATTAGGGAAG 180 25 0 36.00 57.487 4.00 3.00 7.513 493 ACCCACAATGTCTTAGCTTTTGTATTA 188 27 0 33.33 60.517 5.00 3.00 7.517 494 ACAATGTCTTAGCTTTTGTATTAGGG 184 26 0 34.62 58.432 5.00 0.00 7.568 495 ATGTCTTAGCTTTTGTATTAGGGAAGG 181 27 0 37.04 60.573 4.00 1.00 7.573 496 CCCACAATGTCTTAGCTTTTGTATTAG 187 27 0 37.04 60.616 5.00 1.00 7.616 497 CCACAATGTCTTAGCTTTTGTATTAGG 186 27 0 37.04 60.616 5.00 0.00 7.616 498 CACAATGTCTTAGCTTTTGTATTAGGG 185 27 0 37.04 60.616 5.00 0.00 7.616 499 ATTCTTAGGGAACATCTTCAGTGTGTA 130 27 0 37.04 60.637 6.00 2.00 7.637 500 GTGGTACACCCACAATGTCTTAGCT 195 25 0 48.00 62.652 5.00 4.00 7.652 501 TTAGCTTTTGTATTAGGGAAGGTCTTC 176 27 0 37.04 60.660 6.00 6.00 7.660 502 CACCCACAATGTCTTAGCTTTTGTAT 189 26 0 38.46 61.720 5.00 2.00 7.720 503 AGCTTTTGTATTAGGGAAGGTCTTCA 174 26 0 38.46 61.823 6.00 4.00 7.823 504 GCTTTTGTATTAGGGAAGGTCTTCAG 173 26 0 42.31 61.823 6.00 2.00 7.823 505 AGCTGTAGGAAGTGTGTGGTACACC 210 25 0 52.00 62.860 5.00 4.00 7.860 506 AATGTCTTAGCTTTTGTATTAGGGAA 182 26 0 30.77 58.116 4.00 1.00 7.884 507 TGTCTTAGCTTTTGTATTAGGGAAGGT 180 27 0 37.04 60.995 4.00 3.00 7.995 508 CTTAGGGAACATCTTCAGTGTGTACAT 127 27 0 40.74 61.005 6.00 4.00 8.005 509 GTATTAGGGAAGGTCTTCAGTGCATT 167 26 0 42.31 62.082 6.00 4.00 8.082 510 AATGTCTTAGCTTTTGTATTAGGGAAG 182 27 0 33.33 58.903 4.00 3.00 8.097 511 TTCTTAGGGAACATCTTCAGTGTGTAC 129 27 0 40.74 61.101 6.00 4.00 8.101 512 ATGTCTTAGCTTTTGTATTAGGGAAG 181 26 0 34.62 57.811 4.00 3.00 8.189 513 GTACACCCACAATGTCTTAGCTTTTG 192 26 0 42.31 62.195 5.00 3.00 8.195 514 TACACCCACAATGTCTTAGCTTTTGT 191 26 0 38.46 62.195 5.00 5.00 8.195 515 ACACCCACAATGTCTTAGCTTTTGTA 190 26 0 38.46 62.195 5.00 3.00 8.195 516 CCACAATGTCTTAGCTTTTGTATTAG 186 26 0 34.62 57.766 5.00 1.00 8.234 517 CACAATGTCTTAGCTTTTGTATTAGG 185 26 0 34.62 57.766 5.00 0.00 8.234 518 CACATTCTTAGGGAACATCTTCAGTG 133 26 0 42.31 62.327 6.00 3.00 8.327 519 CATTCTTAGGGAACATCTTCAGTGTG 131 26 0 42.31 62.327 6.00 2.00 8.327 520 CTTTTGTATTAGGGAAGGTCTTCAGTG 172 27 0 40.74 61.738 6.00 3.00 8.738 521 GGTACACCCACAATGTCTTAGCTTTT 193 26 0 42.31 62.764 5.00 1.00 8.764 522 TAGCTTTTGTATTAGGGAAGGTCTTCA 175 27 0 37.04 61.778 6.00 4.00 8.778 523 ACAGCTGTAGGAAGTGTGTGGTACAC 212 26 0 50.00 62.885 8.00 5.00 8.885 524 CCACATTCTTAGGGAACATCTTCAGT 134 26 0 42.31 62.911 6.00 4.00 8.911 525 TATTAGGGAAGGTCTTCAGTGCATTC 166 26 0 42.31 62.924 6.00 2.00 8.924 526 TTTGTATTAGGGAAGGTCTTCAGTGC 170 26 0 42.31 62.934 6.00 2.00 8.934 527 TACACCCACAATGTCTTAGCTTTTGTA 191 27 0 37.04 62.136 5.00 3.00 9.136 528 ACACCCACAATGTCTTAGCTTTTGTAT 190 27 0 37.04 62.320 5.00 2.00 9.320 529 TCTTAGGGAACATCTTCAGTGTGTACA 128 27 0 40.74 62.355 6.00 6.00 9.355 530 GCTTTTGTATTAGGGAAGGTCTTCAGT 173 27 0 40.74 62.397 6.00 4.00 9.397 531 AGCTTTTGTATTAGGGAAGGTCTTCAG 174 27 0 40.74 62.483 6.00 2.00 9.483 532 TTAGGGAACATCTTCAGTGTGTACATG 126 27 0 40.74 62.561 6.00 5.00 9.561 533 CACCCACAATGTCTTAGCTTTTGTATT 189 27 0 37.04 62.689 5.00 1.00 9.689 534 GTACACCCACAATGTCTTAGCTTTTGT 192 27 0 40.74 62.787 5.00 5.00 9.787 535 CACATTCTTAGGGAACATCTTCAGTGT 133 27 0 40.74 62.934 6.00 4.00 9.934 536 ACATTCTTAGGGAACATCTTCAGTGTG 132 27 0 40.74 62.934 6.00 2.00 9.934 537 CATTCTTAGGGAACATCTTCAGTGTGT 131 27 0 40.74 62.934 6.00 2.00 9.934 primer3-2.3.6/test/primer1_list_last/SP01-21.txt.for0000664000077300007730000005577012231156243020737 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGTGTCTTGTGGGTGTGTCTG 59 21 0 52.38 60.680 1.00 1.00 1.680 1 TGTCTTGTGGGTGTGTCTGTT 61 21 0 47.62 59.059 1.00 0.00 1.941 2 GTGTCTTGTGGGTGTGTCTGTT 60 22 0 50.00 59.972 1.00 0.00 2.028 3 GAACATGTGTGGCATTGTGT 1 20 0 45.00 57.775 6.00 0.00 2.225 4 GTGTCTTGTGGGTGTGTCTGT 60 21 0 52.38 58.472 1.00 0.00 2.528 5 TGTCTTGTGGGTGTGTCTGT 61 20 0 50.00 57.431 1.00 0.00 2.569 6 GTGTCTTGTGGGTGTGTCTG 60 20 0 55.00 57.431 1.00 1.00 2.569 7 TGTGTCTTGTGGGTGTGTCT 59 20 0 50.00 57.431 1.00 0.00 2.569 8 TGTCTTGTGGGTGTGTCTGTTA 61 22 0 45.45 59.108 2.00 2.00 2.892 9 TTGTGGGTGTGTCTGTTAAACA 65 22 0 40.91 58.997 7.00 7.00 3.003 10 GTGTCTTGTGGGTGTGTCTGTTA 60 23 0 47.83 59.984 2.00 2.00 3.016 11 CTTGTGGGTGTGTCTGTTAAACA 64 23 0 43.48 59.981 7.00 7.00 3.019 12 GAACATGTGTGGCATTGTGTA 1 21 0 42.86 57.880 6.00 2.00 3.120 13 TGTCTTGTGGGTGTGTCTGTTAA 61 23 0 43.48 60.493 4.00 4.00 3.493 14 TGTGGGTGTGTCTGTTAAACA 66 21 0 42.86 57.489 7.00 7.00 3.511 15 TGTGTCTTGTGGGTGTGTCTGT 59 22 0 50.00 61.549 1.00 0.00 3.549 16 AAACATGTGTGGTATTGTGCA 82 21 0 38.10 57.391 6.00 4.00 3.609 17 TTGTGGGTGTGTCTGTTAAACAT 65 23 0 39.13 59.319 7.00 5.00 3.681 18 GAACATGTGTGGCATTGTGTAT 1 22 0 40.91 58.283 6.00 2.00 3.717 19 TGGGTGTGTCTGTTAAACATGTG 68 23 0 43.48 60.746 7.00 4.00 3.746 20 TGTGGGTGTGTCTGTTAAACATG 66 23 0 43.48 60.746 7.00 5.00 3.746 21 TCTTGTGGGTGTGTCTGTTAAA 63 22 0 40.91 58.187 4.00 3.00 3.813 22 AGCTTCTTGACTGTGTGTTGTG 129 22 0 45.45 58.075 4.00 0.00 3.925 23 TCTTGTGGGTGTGTCTGTTAAAC 63 23 0 43.48 59.063 5.00 5.00 3.937 24 GTCTTGTGGGTGTGTCTGTTAAA 62 23 0 43.48 59.063 4.00 3.00 3.937 25 TGGATGTAGCTTCTTGACTGTG 122 22 0 45.45 58.036 4.00 1.00 3.964 26 AGCTTCTTGACTGTGTGTTGTGT 129 23 0 43.48 58.981 4.00 0.00 4.019 27 GGATGTAGCTTCTTGACTGTGTG 123 23 0 47.83 58.925 4.00 0.00 4.075 28 TGGATGTAGCTTCTTGACTGTGT 122 23 0 43.48 58.925 4.00 1.00 4.075 29 TTAAACATGTGTGGTATTGTGCA 80 23 0 34.78 58.925 6.00 4.00 4.075 30 GGGTGTGTCTGTTAAACATGTG 69 22 0 45.45 57.912 7.00 4.00 4.088 31 TGGGTGTGTCTGTTAAACATGT 68 22 0 40.91 57.912 7.00 6.00 4.088 32 GTGGGTGTGTCTGTTAAACATG 67 22 0 45.45 57.912 7.00 5.00 4.088 33 TGTGGGTGTGTCTGTTAAACAT 66 22 0 40.91 57.912 7.00 5.00 4.088 34 AACATGTGTGGCATTGTGTATACA 2 24 0 37.50 60.103 8.00 8.00 4.103 35 GTCTTGTGGGTGTGTCTGTTAAAC 62 24 0 45.83 59.875 5.00 5.00 4.125 36 CATGTGTGGCATTGTGTATACA 4 22 0 40.91 57.850 8.00 8.00 4.150 37 AAACATGTGTGGTATTGTGCAT 82 22 0 36.36 57.811 6.00 2.00 4.189 38 GGGTGTGTCTGTTAAACATGTGT 69 23 0 43.48 58.808 7.00 6.00 4.192 39 GTGGGTGTGTCTGTTAAACATGT 67 23 0 43.48 58.808 7.00 6.00 4.192 40 ACATGTGTGGCATTGTGTATACA 3 23 0 39.13 58.766 8.00 8.00 4.234 41 CTTGTGGGTGTGTCTGTTAAACAT 64 24 0 41.67 60.239 7.00 5.00 4.239 42 GGATGTAGCTTCTTGACTGTGTGT 123 24 0 45.83 59.748 4.00 0.00 4.252 43 GTTAAACATGTGTGGTATTGTGCA 79 24 0 37.50 59.733 6.00 4.00 4.267 44 TGTTAAACATGTGTGGTATTGTGC 78 24 0 37.50 59.733 7.00 2.00 4.267 45 GTCTTGTGGGTGTGTCTGTTAA 62 22 0 45.45 57.635 4.00 4.00 4.365 46 TAAACATGTGTGGTATTGTGCA 81 22 0 36.36 57.504 6.00 4.00 4.496 47 TGTGTGTTGTGTATGGATGTAGC 109 23 0 43.48 58.499 2.00 2.00 4.501 48 TGTGTGTTGTGTATGGATGTAGCT 109 24 0 41.67 59.493 4.00 4.00 4.507 49 ATGGATGTAGCTTCTTGACTGTG 121 23 0 43.48 58.408 4.00 1.00 4.592 50 GAACATGTGTGGCATTGTGTATA 1 23 0 39.13 58.360 6.00 4.00 4.640 51 TTGTGCATATACATGTGTGTTGTG 96 24 0 37.50 59.345 6.00 2.00 4.655 52 ATGGATGTAGCTTCTTGACTGTGT 121 24 0 41.67 59.238 4.00 1.00 4.762 53 TTAAACATGTGTGGTATTGTGCAT 80 24 0 33.33 59.233 6.00 2.00 4.767 54 GAACATGTGTGGCATTGTGTATAC 1 24 0 41.67 59.208 6.00 6.00 4.792 55 CTTGTGGGTGTGTCTGTTAAAC 64 22 0 45.45 57.176 5.00 5.00 4.824 56 TAGCTTCTTGACTGTGTGTTGTG 128 23 0 43.48 58.160 4.00 0.00 4.840 57 TGTAGCTTCTTGACTGTGTGTTG 126 23 0 43.48 58.160 4.00 0.00 4.840 58 TGTGTTGTGTATGGATGTAGCTTC 111 24 0 41.67 59.091 4.00 4.00 4.909 59 TAGCTTCTTGACTGTGTGTTGTGT 128 24 0 41.67 59.030 4.00 0.00 4.970 60 GTAGCTTCTTGACTGTGTGTTGTG 127 24 0 45.83 59.030 4.00 0.00 4.970 61 TGTAGCTTCTTGACTGTGTGTTGT 126 24 0 41.67 59.030 4.00 0.00 4.970 62 TGTGTCTGTTAAACATGTGTGG 72 22 0 40.91 57.024 7.00 2.00 4.976 63 TGTGTTGTGTATGGATGTAGCTTCT 111 25 0 40.00 60.002 4.00 2.00 5.002 64 GTTAAACATGTGTGGTATTGTGCAT 79 25 0 36.00 59.991 6.00 2.00 5.009 65 TGTGTCTGTTAAACATGTGTGGT 72 23 0 39.13 57.983 7.00 0.00 5.017 66 GTGTGTCTGTTAAACATGTGTGG 71 23 0 43.48 57.983 7.00 2.00 5.017 67 GGTGTGTCTGTTAAACATGTGTG 70 23 0 43.48 57.983 7.00 4.00 5.017 68 TGTGCATATACATGTGTGTTGTG 97 23 0 39.13 57.923 6.00 2.00 5.077 69 AAACATGTGTGGTATTGTGCATA 82 23 0 34.78 57.903 6.00 4.00 5.097 70 TAAACATGTGTGGTATTGTGCAT 81 23 0 34.78 57.903 6.00 2.00 5.097 71 GTGTGTTGTGTATGGATGTAGCTTC 110 25 0 44.00 59.880 4.00 4.00 5.120 72 GTGTGTCTGTTAAACATGTGTGGT 71 24 0 41.67 58.868 7.00 0.00 5.132 73 TTGTGCATATACATGTGTGTTGTGT 96 25 0 36.00 60.151 6.00 0.00 5.151 74 ATGTGTGTTGTGTATGGATGTAGC 108 24 0 41.67 58.842 2.00 2.00 5.158 75 GTAGCTTCTTGACTGTGTGTTGTGT 127 25 0 44.00 59.835 4.00 0.00 5.165 76 TTGTGTATGGATGTAGCTTCTTGA 115 24 0 37.50 58.834 4.00 2.00 5.166 77 TGTGCATATACATGTGTGTTGTGT 97 24 0 37.50 58.828 6.00 0.00 5.172 78 ATGTGTGTTGTGTATGGATGTAGCT 108 25 0 40.00 59.772 4.00 4.00 5.228 79 GTGTCTTGTGGGTGTGTCTGTTAA 60 24 0 45.83 61.270 4.00 4.00 5.270 80 GATGTAGCTTCTTGACTGTGTGTTG 124 25 0 44.00 60.272 4.00 0.00 5.272 81 ACATGTGTGTTGTGTATGGATGT 106 23 0 39.13 57.646 6.00 0.00 5.354 82 ATTGTGCATATACATGTGTGTTGTG 95 25 0 36.00 59.637 6.00 2.00 5.363 83 TTGTGTATGGATGTAGCTTCTTGAC 115 25 0 40.00 59.615 4.00 1.00 5.385 84 GTTGTGTATGGATGTAGCTTCTTGA 114 25 0 40.00 59.615 4.00 2.00 5.385 85 TGTTGTGTATGGATGTAGCTTCTTG 113 25 0 40.00 60.458 4.00 2.00 5.458 86 ATGTAGCTTCTTGACTGTGTGTTG 125 24 0 41.67 58.520 4.00 0.00 5.480 87 GGTGTGTCTGTTAAACATGTGTGG 70 24 0 45.83 61.509 7.00 2.00 5.509 88 GGGTGTGTCTGTTAAACATGTGTG 69 24 0 45.83 61.509 7.00 4.00 5.509 89 TGGGTGTGTCTGTTAAACATGTGT 68 24 0 41.67 61.509 7.00 6.00 5.509 90 GTGGGTGTGTCTGTTAAACATGTG 67 24 0 45.83 61.509 7.00 4.00 5.509 91 TGTGGGTGTGTCTGTTAAACATGT 66 24 0 41.67 61.509 7.00 6.00 5.509 92 TATGGATGTAGCTTCTTGACTGTG 120 24 0 41.67 58.476 4.00 1.00 5.524 93 TGTATGGATGTAGCTTCTTGACTG 118 24 0 41.67 58.476 4.00 1.00 5.524 94 TGTGTATGGATGTAGCTTCTTGA 116 23 0 39.13 57.467 4.00 2.00 5.533 95 CTGTTAAACATGTGTGGTATTGTGC 77 25 0 40.00 60.599 7.00 2.00 5.599 96 TGGATGTAGCTTCTTGACTGTGTG 122 24 0 45.83 61.619 4.00 0.00 5.619 97 AACATGTGTGGCATTGTGTATAC 2 23 0 39.13 57.373 6.00 6.00 5.627 98 TGTGTATGGATGTAGCTTCTTGAC 116 24 0 41.67 58.339 4.00 1.00 5.661 99 ATGTAGCTTCTTGACTGTGTGTTGT 125 25 0 40.00 59.333 4.00 0.00 5.667 100 TGGTATTGTGCATATACATGTGTG 91 24 0 37.50 58.315 6.00 4.00 5.685 101 TGTGGTATTGTGCATATACATGTG 89 24 0 37.50 58.315 6.00 4.00 5.685 102 TGTGTGGTATTGTGCATATACATG 87 24 0 37.50 58.315 6.00 6.00 5.685 103 CATGTGTGGTATTGTGCATATACA 85 24 0 37.50 58.315 6.00 6.00 5.685 104 TATGGATGTAGCTTCTTGACTGTGT 120 25 0 40.00 59.274 4.00 1.00 5.726 105 GTATGGATGTAGCTTCTTGACTGTG 119 25 0 44.00 59.274 4.00 1.00 5.726 106 TGTATGGATGTAGCTTCTTGACTGT 118 25 0 40.00 59.274 4.00 3.00 5.726 107 GTGTATGGATGTAGCTTCTTGACTG 117 25 0 44.00 59.274 4.00 1.00 5.726 108 TGTGTATGGATGTAGCTTCTTGACT 116 25 0 40.00 59.274 4.00 1.00 5.726 109 AGCTTCTTGACTGTGTGTTGTGTG 129 24 0 45.83 61.730 4.00 0.00 5.730 110 TTAAACATGTGTGGTATTGTGCATA 80 25 0 32.00 59.268 6.00 4.00 5.732 111 TCTTGTGGGTGTGTCTGTTAAACA 63 24 0 41.67 61.733 7.00 7.00 5.733 112 TGTCTTGTGGGTGTGTCTGTTAAA 61 24 0 41.67 61.733 4.00 3.00 5.733 113 AAACATGTGTGGTATTGTGCATAT 82 24 0 33.33 58.262 6.00 4.00 5.738 114 TGTGTGTTGTGTATGGATGTAGCTT 109 25 0 40.00 60.742 4.00 2.00 5.742 115 TGTGTTGTGTATGGATGTAGCTT 111 23 0 39.13 57.257 4.00 2.00 5.743 116 TGTGTCTTGTGGGTGTGTCTGTT 59 23 0 47.83 62.831 1.00 0.00 5.831 117 GTGTGTTGTGTATGGATGTAGCTT 110 24 0 41.67 58.137 4.00 2.00 5.863 118 GCATATACATGTGTGTTGTGTATGG 100 25 0 40.00 59.136 6.00 3.00 5.864 119 TGGTATTGTGCATATACATGTGTGT 91 25 0 36.00 59.136 6.00 6.00 5.864 120 GTGGTATTGTGCATATACATGTGTG 90 25 0 40.00 59.136 6.00 4.00 5.864 121 TGTGGTATTGTGCATATACATGTGT 89 25 0 36.00 59.136 6.00 6.00 5.864 122 GTGTGGTATTGTGCATATACATGTG 88 25 0 40.00 59.136 6.00 4.00 5.864 123 TGTGTGGTATTGTGCATATACATGT 87 25 0 36.00 59.136 6.00 6.00 5.864 124 ACATGTGTGGTATTGTGCATATACA 84 25 0 36.00 59.136 6.00 6.00 5.864 125 TGTGTCTGTTAAACATGTGTGGTA 72 24 0 37.50 58.069 7.00 2.00 5.931 126 GTTAAACATGTGTGGTATTGTGC 79 23 0 39.13 57.060 6.00 2.00 5.940 127 GGATGTAGCTTCTTGACTGTGTGTT 123 25 0 44.00 60.962 4.00 0.00 5.962 128 TTGTGTATGGATGTAGCTTCTTG 115 23 0 39.13 57.036 4.00 2.00 5.964 129 TTGTGGGTGTGTCTGTTAAACATG 65 24 0 41.67 61.967 7.00 5.00 5.967 130 GTTAAACATGTGTGGTATTGTGCATA 79 26 0 34.62 60.001 6.00 4.00 6.001 131 TAAACATGTGTGGTATTGTGCATA 81 24 0 33.33 57.988 6.00 4.00 6.012 132 GTATGGATGTAGCTTCTTGACTGTGT 119 26 0 42.31 60.018 4.00 1.00 6.018 133 GTGTATGGATGTAGCTTCTTGACTGT 117 26 0 42.31 60.018 4.00 3.00 6.018 134 GTGTGTCTGTTAAACATGTGTGGTA 71 25 0 40.00 58.919 7.00 2.00 6.081 135 TCTGTTAAACATGTGTGGTATTGTG 76 25 0 36.00 58.912 7.00 0.00 6.088 136 TGTCTGTTAAACATGTGTGGTATTG 74 25 0 36.00 58.912 7.00 0.00 6.088 137 GTTGTGTATGGATGTAGCTTCTTG 114 24 0 41.67 57.904 4.00 2.00 6.096 138 TGTTGTGTATGGATGTAGCTTCTT 113 24 0 37.50 57.904 4.00 4.00 6.096 139 GTGGTATTGTGCATATACATGTGTGT 90 26 0 38.46 59.900 6.00 6.00 6.100 140 GTGTGGTATTGTGCATATACATGTGT 88 26 0 38.46 59.900 6.00 6.00 6.100 141 CATGTGTGTTGTGTATGGATGTAG 107 24 0 41.67 57.896 4.00 0.00 6.104 142 TGTGCATATACATGTGTGTTGTGTA 97 25 0 36.00 58.882 6.00 2.00 6.118 143 TTGTGCATATACATGTGTGTTGTGTA 96 26 0 34.62 60.156 6.00 2.00 6.156 144 ACATGTGTGTTGTGTATGGATGTAG 106 25 0 40.00 58.761 6.00 0.00 6.239 145 ACATGTGTGTTGTGTATGGATGTA 106 24 0 37.50 57.747 6.00 2.00 6.253 146 TACATGTGTGTTGTGTATGGATGT 105 24 0 37.50 57.747 6.00 0.00 6.253 147 GTGTTGTGTATGGATGTAGCTTCTT 112 25 0 40.00 58.712 4.00 4.00 6.288 148 GTCTGTTAAACATGTGTGGTATTGTG 75 26 0 38.46 59.680 7.00 0.00 6.320 149 TGTCTGTTAAACATGTGTGGTATTGT 74 26 0 34.62 59.680 7.00 0.00 6.320 150 GTGTCTGTTAAACATGTGTGGTATTG 73 26 0 38.46 59.680 7.00 0.00 6.320 151 TGTGTCTGTTAAACATGTGTGGTATT 72 26 0 34.62 59.680 7.00 1.00 6.320 152 TATTGTGCATATACATGTGTGTTGTG 94 26 0 34.62 59.659 6.00 2.00 6.341 153 GTTGTGTATGGATGTAGCTTCTTGAC 114 26 0 42.31 60.343 4.00 1.00 6.343 154 GGTATTGTGCATATACATGTGTGTTG 92 26 0 38.46 60.346 6.00 4.00 6.346 155 TGGTATTGTGCATATACATGTGTGTT 91 26 0 34.62 60.346 6.00 4.00 6.346 156 AACATGTGTGGTATTGTGCATATACA 83 26 0 34.62 60.346 6.00 6.00 6.346 157 ATGTGTGGTATTGTGCATATACATG 86 25 0 36.00 58.650 6.00 6.00 6.350 158 CATGTGTGGTATTGTGCATATACAT 85 25 0 36.00 58.650 6.00 4.00 6.350 159 GATGTAGCTTCTTGACTGTGTGTT 124 24 0 41.67 57.626 4.00 0.00 6.374 160 ATTGTGCATATACATGTGTGTTGTGT 95 26 0 34.62 60.393 6.00 0.00 6.393 161 TTGTGTATGGATGTAGCTTCTTGACT 115 26 0 38.46 60.455 4.00 1.00 6.455 162 TTAAACATGTGTGGTATTGTGCATAT 80 26 0 30.77 59.536 6.00 4.00 6.464 163 CATGTGTGTTGTGTATGGATGTAGC 107 25 0 44.00 61.472 4.00 2.00 6.472 164 TGTGTCTGTTAAACATGTGTGGTAT 72 25 0 36.00 58.424 7.00 2.00 6.576 165 ATGTGTGGTATTGTGCATATACATGT 86 26 0 34.62 59.420 6.00 6.00 6.580 166 ACATGTGTGGTATTGTGCATATACAT 84 26 0 34.62 59.420 6.00 4.00 6.580 167 GTGTTGTGTATGGATGTAGCTTCT 112 24 0 41.67 57.400 4.00 2.00 6.600 168 TGCATATACATGTGTGTTGTGTATG 99 25 0 36.00 58.378 6.00 5.00 6.622 169 AAACATGTGTGGTATTGTGCATATA 82 25 0 32.00 58.331 6.00 4.00 6.669 170 TAAACATGTGTGGTATTGTGCATAT 81 25 0 32.00 58.331 6.00 4.00 6.669 171 TAGCTTCTTGACTGTGTGTTGTGTG 128 25 0 44.00 61.678 4.00 0.00 6.678 172 TGTAGCTTCTTGACTGTGTGTTGTG 126 25 0 44.00 61.678 4.00 0.00 6.678 173 GTGTGTTGTGTATGGATGTAGCTTCT 110 26 0 42.31 60.727 4.00 2.00 6.727 174 TGTGTCTTGTGGGTGTGTCTGTTA 59 24 0 45.83 62.734 2.00 2.00 6.734 175 ATACATGTGTGTTGTGTATGGATG 104 24 0 37.50 57.241 6.00 0.00 6.759 176 GTGTGTCTGTTAAACATGTGTGGTAT 71 26 0 38.46 59.220 7.00 2.00 6.780 177 ATGGATGTAGCTTCTTGACTGTGTG 121 25 0 44.00 61.791 4.00 0.00 6.791 178 GAACATGTGTGGCATTGTGTATACA 1 25 0 40.00 61.810 8.00 8.00 6.810 179 GTGCATATACATGTGTGTTGTGTATG 98 26 0 38.46 59.190 6.00 5.00 6.810 180 TGTGCATATACATGTGTGTTGTGTAT 97 26 0 34.62 59.190 6.00 3.00 6.810 181 GCATATACATGTGTGTTGTGTATGGA 100 26 0 38.46 60.811 6.00 1.00 6.811 182 CTGTTAAACATGTGTGGTATTGTG 77 24 0 37.50 57.140 7.00 0.00 6.860 183 ATACATGTGTGTTGTGTATGGATGT 104 25 0 36.00 58.124 6.00 0.00 6.876 184 TCTTGTGGGTGTGTCTGTTAAACAT 63 25 0 40.00 61.898 7.00 5.00 6.898 185 AAACATGTGTGGTATTGTGCATATAC 82 26 0 34.62 59.095 6.00 6.00 6.905 186 ATGTGTGTTGTGTATGGATGTAGCTT 108 26 0 38.46 60.948 4.00 2.00 6.948 187 AACATGTGTGGTATTGTGCATATA 83 24 0 33.33 57.002 6.00 4.00 6.998 188 GATGTAGCTTCTTGACTGTGTGTTGT 124 26 0 42.31 61.001 4.00 0.00 7.001 189 CATGTGTGGTATTGTGCATATACATG 85 26 0 38.46 61.147 6.00 6.00 7.147 190 GGTATTGTGCATATACATGTGTGTT 92 25 0 36.00 57.851 6.00 4.00 7.149 191 AACATGTGTGGTATTGTGCATATAC 83 25 0 36.00 57.851 6.00 6.00 7.149 192 GTGTTGTGTATGGATGTAGCTTCTTG 112 26 0 42.31 61.157 4.00 2.00 7.157 193 TGTGTTGTGTATGGATGTAGCTTCTT 111 26 0 38.46 61.157 4.00 4.00 7.157 194 TACATGTGTGTTGTGTATGGATGTA 105 25 0 36.00 57.841 6.00 2.00 7.159 195 TACATGTGTGTTGTGTATGGATGTAG 105 26 0 38.46 58.815 6.00 0.00 7.185 196 GGTGTGTCTGTTAAACATGTGTGGT 70 25 0 44.00 62.217 7.00 0.00 7.217 197 GTGGGTGTGTCTGTTAAACATGTGT 67 25 0 44.00 62.217 7.00 6.00 7.217 198 GTTAAACATGTGTGGTATTGTGCATAT 79 27 0 33.33 60.227 6.00 4.00 7.227 199 CATATACATGTGTGTTGTGTATGGATG 101 27 0 37.04 60.230 6.00 0.00 7.230 200 TGTTAAACATGTGTGGTATTGTGCA 78 25 0 36.00 62.264 7.00 4.00 7.264 201 TGGATGTAGCTTCTTGACTGTGTGT 122 25 0 44.00 62.322 4.00 0.00 7.322 202 TTGTGCATATACATGTGTGTTGTGTAT 96 27 0 33.33 60.389 6.00 3.00 7.389 203 ATTGTGCATATACATGTGTGTTGTGTA 95 27 0 33.33 60.389 6.00 2.00 7.389 204 TATTGTGCATATACATGTGTGTTGTGT 94 27 0 33.33 60.389 6.00 0.00 7.389 205 GTATTGTGCATATACATGTGTGTTGTG 93 27 0 37.04 60.389 6.00 2.00 7.389 206 GTGTCTGTTAAACATGTGTGGTATTGT 73 27 0 37.04 60.396 7.00 0.00 7.396 207 GTGTGTCTGTTAAACATGTGTGGTATT 71 27 0 37.04 60.396 7.00 1.00 7.396 208 TGTTGTGTATAAATGTTGTGTCTTCA 32 26 0 30.77 58.596 4.00 1.00 7.404 209 GTCTTGTGGGTGTGTCTGTTAAACA 62 25 0 44.00 62.427 7.00 7.00 7.427 210 TGTCTTGTGGGTGTGTCTGTTAAAC 61 25 0 44.00 62.427 5.00 5.00 7.427 211 GTGTCTTGTGGGTGTGTCTGTTAAA 60 25 0 44.00 62.427 4.00 3.00 7.427 212 TTAAACATGTGTGGTATTGTGCATATA 80 27 0 29.63 59.560 6.00 4.00 7.440 213 AGCTTCTTGACTGTGTGTTGTGTGT 129 25 0 44.00 62.443 4.00 0.00 7.443 214 AACATGTGTGGTATTGTGCATATACAT 83 27 0 33.33 60.562 6.00 4.00 7.562 215 TAAACATGTGTGGTATTGTGCATATA 81 26 0 30.77 58.395 6.00 4.00 7.605 216 GTGTTGTGTATAAATGTTGTGTCTTCA 31 27 0 33.33 59.358 4.00 1.00 7.642 217 TGTGTTGTGTATAAATGTTGTGTCTTC 30 27 0 33.33 59.358 4.00 0.00 7.642 218 TTGTGGGTGTGTCTGTTAAACATGT 65 25 0 40.00 62.648 7.00 6.00 7.648 219 TATACATGTGTGTTGTGTATGGATG 103 25 0 36.00 57.352 6.00 0.00 7.648 220 CATATACATGTGTGTTGTGTATGGA 101 25 0 36.00 57.352 6.00 0.00 7.648 221 TGCATATACATGTGTGTTGTGTATGG 99 26 0 38.46 61.654 6.00 3.00 7.654 222 TGTGGTATTGTGCATATACATGTGTG 89 26 0 38.46 61.654 6.00 4.00 7.654 223 TGTGTGGTATTGTGCATATACATGTG 87 26 0 38.46 61.654 6.00 4.00 7.654 224 TATGGATGTAGCTTCTTGACTGTGTG 120 26 0 42.31 61.740 4.00 0.00 7.740 225 TGTATGGATGTAGCTTCTTGACTGTG 118 26 0 42.31 61.740 4.00 1.00 7.740 226 TGTGTATGGATGTAGCTTCTTGACTG 116 26 0 42.31 61.740 4.00 1.00 7.740 227 CTTGTGGGTGTGTCTGTTAAACATG 64 25 0 44.00 62.749 7.00 5.00 7.749 228 ATACATGTGTGTTGTGTATGGATGTA 104 26 0 34.62 58.200 6.00 2.00 7.800 229 TATACATGTGTGTTGTGTATGGATGT 103 26 0 34.62 58.200 6.00 0.00 7.800 230 ATGTAGCTTCTTGACTGTGTGTTGTG 125 26 0 42.31 61.844 4.00 0.00 7.844 231 GTCTGTTAAACATGTGTGGTATTGT 75 25 0 36.00 57.143 7.00 0.00 7.857 232 GTGTCTGTTAAACATGTGTGGTATT 73 25 0 36.00 57.143 7.00 1.00 7.857 233 TAAACATGTGTGGTATTGTGCATATAC 81 27 0 33.33 59.133 6.00 6.00 7.867 234 ATACATGTGTGTTGTGTATGGATGTAG 104 27 0 37.04 59.113 6.00 0.00 7.887 235 TATTGTGCATATACATGTGTGTTGT 94 25 0 32.00 57.073 6.00 2.00 7.927 236 GTATTGTGCATATACATGTGTGTTG 93 25 0 36.00 57.073 6.00 4.00 7.927 237 GCATATACATGTGTGTTGTGTATGGAT 100 27 0 37.04 61.010 6.00 2.00 8.010 238 GGTATTGTGCATATACATGTGTGTTGT 92 27 0 37.04 61.034 6.00 2.00 8.034 239 GTGGTATTGTGCATATACATGTGTGTT 90 27 0 37.04 61.034 6.00 4.00 8.034 240 TGTTGTGTATGGATGTAGCTTCTTGA 113 26 0 38.46 62.056 4.00 2.00 8.056 241 GTATTGTGCATATACATGTGTGTTGT 93 26 0 34.62 57.913 6.00 2.00 8.087 242 GTTGTGTATGGATGTAGCTTCTTGACT 114 27 0 40.74 61.126 4.00 1.00 8.126 243 GGTGTGTCTGTTAAACATGTGTGGTA 70 26 0 42.31 62.150 7.00 2.00 8.150 244 ACATGTGTGTTGTGTATGGATGTAGC 106 26 0 42.31 62.163 6.00 2.00 8.163 245 TCTGTTAAACATGTGTGGTATTGTGC 76 26 0 38.46 62.185 7.00 2.00 8.185 246 CATGTGTGTTGTGTATGGATGTAGCT 107 26 0 42.31 62.266 4.00 4.00 8.266 247 ATATACATGTGTGTTGTGTATGGATG 102 26 0 34.62 57.730 6.00 0.00 8.270 248 CATATACATGTGTGTTGTGTATGGAT 101 26 0 34.62 57.730 6.00 2.00 8.270 249 TGTGTTGTGTATAAATGTTGTGTCTT 30 26 0 30.77 57.700 4.00 0.00 8.300 250 TAGCTTCTTGACTGTGTGTTGTGTGT 128 26 0 42.31 62.367 4.00 0.00 8.367 251 GTAGCTTCTTGACTGTGTGTTGTGTG 127 26 0 46.15 62.367 4.00 0.00 8.367 252 TGTAGCTTCTTGACTGTGTGTTGTGT 126 26 0 42.31 62.367 4.00 0.00 8.367 253 TGTGTGTTGTGTATGGATGTAGCTTC 109 26 0 42.31 62.368 4.00 4.00 8.368 254 TGTTAAACATGTGTGGTATTGTGCAT 78 26 0 34.62 62.397 7.00 2.00 8.397 255 AAACATGTGTGGTATTGTGCATATACA 82 27 0 33.33 61.445 6.00 6.00 8.445 256 ATGGATGTAGCTTCTTGACTGTGTGT 121 26 0 42.31 62.454 4.00 0.00 8.454 257 ATATACATGTGTGTTGTGTATGGATGT 102 27 0 33.33 58.526 6.00 0.00 8.474 258 GTCTTGTGGGTGTGTCTGTTAAACAT 62 26 0 42.31 62.553 7.00 5.00 8.553 259 TGTGCATATACATGTGTGTTGTGTATG 97 27 0 37.04 61.688 6.00 5.00 8.688 260 TATACATGTGTGTTGTGTATGGATGTA 103 27 0 33.33 58.271 6.00 2.00 8.729 261 GTGTGTTGTGTATGGATGTAGCTTCTT 110 27 0 40.74 61.811 4.00 4.00 8.811 262 ATGTGTGGTATTGTGCATATACATGTG 86 27 0 37.04 61.814 6.00 4.00 8.814 263 CATGTGTGGTATTGTGCATATACATGT 85 27 0 37.04 61.814 6.00 6.00 8.814 264 ACATGTGTGGTATTGTGCATATACATG 84 27 0 37.04 61.814 6.00 6.00 8.814 265 ATGTGTTGTGTATAAATGTTGTGTCTT 29 27 0 29.63 58.041 4.00 0.00 8.959 266 TGTCTGTTAAACATGTGTGGTATTGTG 74 27 0 37.04 62.089 7.00 0.00 9.089 267 TGTGTCTGTTAAACATGTGTGGTATTG 72 27 0 37.04 62.089 7.00 0.00 9.089 268 TACATGTGTGTTGTGTATGGATGTAGC 105 27 0 40.74 62.100 6.00 2.00 9.100 269 GGTGTGTCTGTTAAACATGTGTGGTAT 70 27 0 40.74 62.286 7.00 2.00 9.286 270 GTGCATATACATGTGTGTTGTGTATGG 98 27 0 40.74 62.312 6.00 3.00 9.312 271 TGTGGTATTGTGCATATACATGTGTGT 89 27 0 37.04 62.312 6.00 6.00 9.312 272 GTGTGGTATTGTGCATATACATGTGTG 88 27 0 40.74 62.312 6.00 4.00 9.312 273 TGTGTGGTATTGTGCATATACATGTGT 87 27 0 37.04 62.312 6.00 6.00 9.312 274 TGTTAAACATGTGTGGTATTGTGCATA 78 27 0 33.33 62.329 7.00 4.00 9.329 275 TATGGATGTAGCTTCTTGACTGTGTGT 120 27 0 40.74 62.383 4.00 0.00 9.383 276 GTATGGATGTAGCTTCTTGACTGTGTG 119 27 0 44.44 62.383 4.00 0.00 9.383 277 TGTATGGATGTAGCTTCTTGACTGTGT 118 27 0 40.74 62.383 4.00 1.00 9.383 278 GTGTATGGATGTAGCTTCTTGACTGTG 117 27 0 44.44 62.383 4.00 1.00 9.383 279 TGTGTATGGATGTAGCTTCTTGACTGT 116 27 0 40.74 62.383 4.00 3.00 9.383 280 ATGTGTGTTGTGTATGGATGTAGCTTC 108 27 0 40.74 62.494 4.00 4.00 9.494 281 ATGTAGCTTCTTGACTGTGTGTTGTGT 125 27 0 40.74 62.495 4.00 0.00 9.495 282 TGTTGTGTATGGATGTAGCTTCTTGAC 113 27 0 40.74 62.685 4.00 1.00 9.685 283 GTGTTGTGTATGGATGTAGCTTCTTGA 112 27 0 40.74 62.685 4.00 2.00 9.685 284 TGGTATTGTGCATATACATGTGTGTTG 91 27 0 37.04 62.716 6.00 4.00 9.716 285 TTGTGTATGGATGTAGCTTCTTGACTG 115 27 0 40.74 62.778 4.00 1.00 9.778 286 GTCTGTTAAACATGTGTGGTATTGTGC 75 27 0 40.74 62.806 7.00 2.00 9.806 287 CATATGTGTTGTGTATAAATGTTGTGT 26 27 0 29.63 57.112 6.00 0.00 9.888 288 ACATATGTGTTGTGTATAAATGTTGTG 25 27 0 29.63 57.112 8.00 0.00 9.888 289 CACATATGTGTTGTGTATAAATGTTGT 24 27 0 29.63 57.112 10.00 2.00 9.888 290 ACACATATGTGTTGTGTATAAATGTTG 23 27 0 29.63 57.112 12.00 0.00 9.888 291 ACATGTGTGTTGTGTATGGATGTAGCT 106 27 0 40.74 62.902 6.00 4.00 9.902 primer3-2.3.6/test/primer1_list_last/AGAG.for0000664000077300007730000001537012231156243017705 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 GAGAGAGAGAGAGAGAGAGAGAGAGA 56 26 0 50.00 57.752 0.00 0.00 8.248 1 GAGAGAGAGAGAGAGAGAGAGAGAGA 54 26 0 50.00 57.752 0.00 0.00 8.248 2 GAGAGAGAGAGAGAGAGAGAGAGAGA 52 26 0 50.00 57.752 0.00 0.00 8.248 3 GAGAGAGAGAGAGAGAGAGAGAGAGA 50 26 0 50.00 57.752 0.00 0.00 8.248 4 GAGAGAGAGAGAGAGAGAGAGAGAGA 48 26 0 50.00 57.752 0.00 0.00 8.248 5 GAGAGAGAGAGAGAGAGAGAGAGAGA 46 26 0 50.00 57.752 0.00 0.00 8.248 6 GAGAGAGAGAGAGAGAGAGAGAGAGA 44 26 0 50.00 57.752 0.00 0.00 8.248 7 GAGAGAGAGAGAGAGAGAGAGAGAGA 42 26 0 50.00 57.752 0.00 0.00 8.248 8 GAGAGAGAGAGAGAGAGAGAGAGAGA 40 26 0 50.00 57.752 0.00 0.00 8.248 9 GAGAGAGAGAGAGAGAGAGAGAGAGA 38 26 0 50.00 57.752 0.00 0.00 8.248 10 GAGAGAGAGAGAGAGAGAGAGAGAGA 36 26 0 50.00 57.752 0.00 0.00 8.248 11 GAGAGAGAGAGAGAGAGAGAGAGAGA 34 26 0 50.00 57.752 0.00 0.00 8.248 12 GAGAGAGAGAGAGAGAGAGAGAGAGA 32 26 0 50.00 57.752 0.00 0.00 8.248 13 GAGAGAGAGAGAGAGAGAGAGAGAGA 30 26 0 50.00 57.752 0.00 0.00 8.248 14 GAGAGAGAGAGAGAGAGAGAGAGAGA 28 26 0 50.00 57.752 0.00 0.00 8.248 15 GAGAGAGAGAGAGAGAGAGAGAGAGA 26 26 0 50.00 57.752 0.00 0.00 8.248 16 GAGAGAGAGAGAGAGAGAGAGAGAGA 24 26 0 50.00 57.752 0.00 0.00 8.248 17 GAGAGAGAGAGAGAGAGAGAGAGAGA 22 26 0 50.00 57.752 0.00 0.00 8.248 18 GAGAGAGAGAGAGAGAGAGAGAGAGA 20 26 0 50.00 57.752 0.00 0.00 8.248 19 GAGAGAGAGAGAGAGAGAGAGAGAGA 18 26 0 50.00 57.752 0.00 0.00 8.248 20 GAGAGAGAGAGAGAGAGAGAGAGAGA 16 26 0 50.00 57.752 0.00 0.00 8.248 21 GAGAGAGAGAGAGAGAGAGAGAGAGA 14 26 0 50.00 57.752 0.00 0.00 8.248 22 GAGAGAGAGAGAGAGAGAGAGAGAGA 12 26 0 50.00 57.752 0.00 0.00 8.248 23 GAGAGAGAGAGAGAGAGAGAGAGAGA 10 26 0 50.00 57.752 0.00 0.00 8.248 24 GAGAGAGAGAGAGAGAGAGAGAGAGA 8 26 0 50.00 57.752 0.00 0.00 8.248 25 GAGAGAGAGAGAGAGAGAGAGAGAGA 6 26 0 50.00 57.752 0.00 0.00 8.248 26 GAGAGAGAGAGAGAGAGAGAGAGAGA 4 26 0 50.00 57.752 0.00 0.00 8.248 27 GAGAGAGAGAGAGAGAGAGAGAGAGA 2 26 0 50.00 57.752 0.00 0.00 8.248 28 GAGAGAGAGAGAGAGAGAGAGAGAGAG 56 27 0 51.85 58.701 0.00 0.00 8.299 29 AGAGAGAGAGAGAGAGAGAGAGAGAGA 55 27 0 48.15 58.701 0.00 0.00 8.299 30 GAGAGAGAGAGAGAGAGAGAGAGAGAG 54 27 0 51.85 58.701 0.00 0.00 8.299 31 AGAGAGAGAGAGAGAGAGAGAGAGAGA 53 27 0 48.15 58.701 0.00 0.00 8.299 32 GAGAGAGAGAGAGAGAGAGAGAGAGAG 52 27 0 51.85 58.701 0.00 0.00 8.299 33 AGAGAGAGAGAGAGAGAGAGAGAGAGA 51 27 0 48.15 58.701 0.00 0.00 8.299 34 GAGAGAGAGAGAGAGAGAGAGAGAGAG 50 27 0 51.85 58.701 0.00 0.00 8.299 35 AGAGAGAGAGAGAGAGAGAGAGAGAGA 49 27 0 48.15 58.701 0.00 0.00 8.299 36 GAGAGAGAGAGAGAGAGAGAGAGAGAG 48 27 0 51.85 58.701 0.00 0.00 8.299 37 AGAGAGAGAGAGAGAGAGAGAGAGAGA 47 27 0 48.15 58.701 0.00 0.00 8.299 38 GAGAGAGAGAGAGAGAGAGAGAGAGAG 46 27 0 51.85 58.701 0.00 0.00 8.299 39 AGAGAGAGAGAGAGAGAGAGAGAGAGA 45 27 0 48.15 58.701 0.00 0.00 8.299 40 GAGAGAGAGAGAGAGAGAGAGAGAGAG 44 27 0 51.85 58.701 0.00 0.00 8.299 41 AGAGAGAGAGAGAGAGAGAGAGAGAGA 43 27 0 48.15 58.701 0.00 0.00 8.299 42 GAGAGAGAGAGAGAGAGAGAGAGAGAG 42 27 0 51.85 58.701 0.00 0.00 8.299 43 AGAGAGAGAGAGAGAGAGAGAGAGAGA 41 27 0 48.15 58.701 0.00 0.00 8.299 44 GAGAGAGAGAGAGAGAGAGAGAGAGAG 40 27 0 51.85 58.701 0.00 0.00 8.299 45 AGAGAGAGAGAGAGAGAGAGAGAGAGA 39 27 0 48.15 58.701 0.00 0.00 8.299 46 GAGAGAGAGAGAGAGAGAGAGAGAGAG 38 27 0 51.85 58.701 0.00 0.00 8.299 47 AGAGAGAGAGAGAGAGAGAGAGAGAGA 37 27 0 48.15 58.701 0.00 0.00 8.299 48 GAGAGAGAGAGAGAGAGAGAGAGAGAG 36 27 0 51.85 58.701 0.00 0.00 8.299 49 AGAGAGAGAGAGAGAGAGAGAGAGAGA 35 27 0 48.15 58.701 0.00 0.00 8.299 50 GAGAGAGAGAGAGAGAGAGAGAGAGAG 34 27 0 51.85 58.701 0.00 0.00 8.299 51 AGAGAGAGAGAGAGAGAGAGAGAGAGA 33 27 0 48.15 58.701 0.00 0.00 8.299 52 GAGAGAGAGAGAGAGAGAGAGAGAGAG 32 27 0 51.85 58.701 0.00 0.00 8.299 53 AGAGAGAGAGAGAGAGAGAGAGAGAGA 31 27 0 48.15 58.701 0.00 0.00 8.299 54 GAGAGAGAGAGAGAGAGAGAGAGAGAG 30 27 0 51.85 58.701 0.00 0.00 8.299 55 AGAGAGAGAGAGAGAGAGAGAGAGAGA 29 27 0 48.15 58.701 0.00 0.00 8.299 56 GAGAGAGAGAGAGAGAGAGAGAGAGAG 28 27 0 51.85 58.701 0.00 0.00 8.299 57 AGAGAGAGAGAGAGAGAGAGAGAGAGA 27 27 0 48.15 58.701 0.00 0.00 8.299 58 GAGAGAGAGAGAGAGAGAGAGAGAGAG 26 27 0 51.85 58.701 0.00 0.00 8.299 59 AGAGAGAGAGAGAGAGAGAGAGAGAGA 25 27 0 48.15 58.701 0.00 0.00 8.299 60 GAGAGAGAGAGAGAGAGAGAGAGAGAG 24 27 0 51.85 58.701 0.00 0.00 8.299 61 AGAGAGAGAGAGAGAGAGAGAGAGAGA 23 27 0 48.15 58.701 0.00 0.00 8.299 62 GAGAGAGAGAGAGAGAGAGAGAGAGAG 22 27 0 51.85 58.701 0.00 0.00 8.299 63 AGAGAGAGAGAGAGAGAGAGAGAGAGA 21 27 0 48.15 58.701 0.00 0.00 8.299 64 GAGAGAGAGAGAGAGAGAGAGAGAGAG 20 27 0 51.85 58.701 0.00 0.00 8.299 65 AGAGAGAGAGAGAGAGAGAGAGAGAGA 19 27 0 48.15 58.701 0.00 0.00 8.299 66 GAGAGAGAGAGAGAGAGAGAGAGAGAG 18 27 0 51.85 58.701 0.00 0.00 8.299 67 AGAGAGAGAGAGAGAGAGAGAGAGAGA 17 27 0 48.15 58.701 0.00 0.00 8.299 68 GAGAGAGAGAGAGAGAGAGAGAGAGAG 16 27 0 51.85 58.701 0.00 0.00 8.299 69 AGAGAGAGAGAGAGAGAGAGAGAGAGA 15 27 0 48.15 58.701 0.00 0.00 8.299 70 GAGAGAGAGAGAGAGAGAGAGAGAGAG 14 27 0 51.85 58.701 0.00 0.00 8.299 71 AGAGAGAGAGAGAGAGAGAGAGAGAGA 13 27 0 48.15 58.701 0.00 0.00 8.299 72 GAGAGAGAGAGAGAGAGAGAGAGAGAG 12 27 0 51.85 58.701 0.00 0.00 8.299 73 AGAGAGAGAGAGAGAGAGAGAGAGAGA 11 27 0 48.15 58.701 0.00 0.00 8.299 74 GAGAGAGAGAGAGAGAGAGAGAGAGAG 10 27 0 51.85 58.701 0.00 0.00 8.299 75 AGAGAGAGAGAGAGAGAGAGAGAGAGA 9 27 0 48.15 58.701 0.00 0.00 8.299 76 GAGAGAGAGAGAGAGAGAGAGAGAGAG 8 27 0 51.85 58.701 0.00 0.00 8.299 77 AGAGAGAGAGAGAGAGAGAGAGAGAGA 7 27 0 48.15 58.701 0.00 0.00 8.299 78 GAGAGAGAGAGAGAGAGAGAGAGAGAG 6 27 0 51.85 58.701 0.00 0.00 8.299 79 AGAGAGAGAGAGAGAGAGAGAGAGAGA 5 27 0 48.15 58.701 0.00 0.00 8.299 80 GAGAGAGAGAGAGAGAGAGAGAGAGAG 4 27 0 51.85 58.701 0.00 0.00 8.299 81 AGAGAGAGAGAGAGAGAGAGAGAGAGA 3 27 0 48.15 58.701 0.00 0.00 8.299 82 GAGAGAGAGAGAGAGAGAGAGAGAGAG 2 27 0 51.85 58.701 0.00 0.00 8.299 83 AGAGAGAGAGAGAGAGAGAGAGAGAGA 1 27 0 48.15 58.701 0.00 0.00 8.299 primer3-2.3.6/test/primer1_list_last/SP01-03.txt.for0000664000077300007730000002213012231156243020717 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TTAGAAGGGGTGTCTGGGTG 73 20 0 55.00 59.959 3.00 0.00 0.041 1 CTGCAGGTTTAGAAGGGGTG 65 20 0 55.00 59.728 6.00 0.00 0.272 2 CACCCTGCAGGTTTAGAAGG 61 20 0 55.00 59.728 8.00 0.00 0.272 3 AGCACACCCTGCAGGTTTAG 57 20 0 55.00 60.312 8.00 6.00 0.312 4 TGCAGGTTTAGAAGGGGTGT 66 20 0 50.00 59.592 4.00 0.00 0.408 5 CCTGCAGGTTTAGAAGGGGT 64 20 0 55.00 60.488 8.00 0.00 0.488 6 ACCCTGCAGGTTTAGAAGGG 62 20 0 55.00 60.488 8.00 2.00 0.488 7 CACCCTGTAAACCCTGGCT 8 19 0 57.89 59.974 3.00 3.00 1.026 8 TCTGCACCCTGTAAACCCTG 4 20 0 55.00 61.084 4.00 1.00 1.084 9 CACACCCTGCAGGTTTAGAAG 59 21 0 52.38 59.782 8.00 2.00 1.218 10 CACACCCTGCAGGTTTAGAA 59 20 0 50.00 58.773 8.00 2.00 1.227 11 GCACACCCTGCAGGTTTAGA 58 20 0 55.00 61.236 8.00 4.00 1.236 12 CACTGAGGTCAGCACACCC 47 19 0 63.16 60.311 6.00 2.00 1.311 13 CCACTGAGGTCAGCACACC 46 19 0 63.16 60.311 6.00 4.00 1.311 14 CCCACTGAGGTCAGCACAC 45 19 0 63.16 60.311 6.00 2.00 1.311 15 CCTGCAGGTTTAGAAGGGG 64 19 0 57.89 59.673 8.00 0.00 1.327 16 CCCTGCAGGTTTAGAAGGG 63 19 0 57.89 59.673 8.00 2.00 1.327 17 GCAGGTTTAGAAGGGGTGTC 67 20 0 55.00 58.646 2.00 1.00 1.354 18 ACTGAGGTCAGCACACCCTG 48 20 0 60.00 61.355 6.00 2.00 1.355 19 CACTGAGGTCAGCACACCCT 47 20 0 60.00 61.355 6.00 2.00 1.355 20 GCAGGTTTAGAAGGGGTGTCT 67 21 0 52.38 59.630 3.00 0.00 1.370 21 CTGAGGTCAGCACACCCTG 49 19 0 63.16 60.461 6.00 2.00 1.461 22 ATCTGCACCCTGTAAACCCT 3 20 0 50.00 58.525 4.00 0.00 1.475 23 TTTAGAAGGGGTGTCTGGGT 72 20 0 50.00 58.498 3.00 0.00 1.502 24 GTTTAGAAGGGGTGTCTGGG 71 20 0 55.00 58.498 3.00 0.00 1.502 25 GGTTTAGAAGGGGTGTCTGG 70 20 0 55.00 58.498 3.00 0.00 1.502 26 AGGTTTAGAAGGGGTGTCTGG 69 21 0 52.38 59.488 3.00 0.00 1.512 27 CTGCAGGTTTAGAAGGGGTGT 65 21 0 52.38 60.534 6.00 0.00 1.534 28 ACACCCTGCAGGTTTAGAAGG 60 21 0 52.38 60.534 8.00 0.00 1.534 29 GTTTAGAAGGGGTGTCTGGGT 71 21 0 52.38 59.355 3.00 0.00 1.645 30 GCACACCCTGCAGGTTTAG 58 19 0 57.89 59.303 8.00 6.00 1.697 31 AGCACACCCTGCAGGTTTA 57 19 0 52.63 59.303 8.00 4.00 1.697 32 CACCCTGTAAACCCTGGCTC 8 20 0 60.00 61.826 3.00 1.00 1.826 33 CCTGTAAACCCTGGCTCCT 11 19 0 57.89 59.148 3.00 0.00 1.852 34 CTGCACCCTGTAAACCCTG 5 19 0 57.89 59.146 4.00 1.00 1.854 35 CCTGTAAACCCTGGCTCCTG 11 20 0 60.00 61.944 3.00 2.00 1.944 36 GACACCCCACTGAGGTCAG 40 19 0 63.16 59.044 7.00 6.00 1.956 37 ACCCTGTAAACCCTGGCTC 9 19 0 57.89 59.003 3.00 1.00 1.997 38 CTGTAAACCCTGGCTCCTGC 12 20 0 60.00 62.090 3.00 2.00 2.090 39 ACCCCACTGAGGTCAGCAC 43 19 0 63.16 61.171 7.00 1.00 2.171 40 ACACCCCACTGAGGTCAGC 41 19 0 63.16 61.171 7.00 4.00 2.171 41 TGTAAACCCTGGCTCCTGC 13 19 0 57.89 61.192 3.00 2.00 2.192 42 CAGCACACCCTGCAGGTTTA 56 20 0 55.00 62.212 8.00 4.00 2.212 43 CCCCACTGAGGTCAGCAC 44 18 0 66.67 60.252 6.00 1.00 2.252 44 ACCCCACTGAGGTCAGCA 43 18 0 61.11 60.252 7.00 2.00 2.252 45 CACCCCACTGAGGTCAGC 42 18 0 66.67 60.252 7.00 4.00 2.252 46 TTTAGAAGGGGTGTCTGGGTG 72 21 0 52.38 61.267 3.00 0.00 2.267 47 TGCAGGTTTAGAAGGGGTG 66 19 0 52.63 58.707 4.00 0.00 2.293 48 CAGGTTTAGAAGGGGTGTCTG 68 21 0 52.38 58.705 3.00 1.00 2.295 49 ATCTGCACCCTGTAAACCCTG 3 21 0 52.38 61.297 4.00 1.00 2.297 50 TAGAAGGGGTGTCTGGGTGC 74 20 0 60.00 62.397 3.00 2.00 2.397 51 TGCAGGTTTAGAAGGGGTGTC 66 21 0 52.38 61.411 4.00 1.00 2.411 52 GAGGTCAGCACACCCTGC 51 18 0 66.67 60.425 4.00 2.00 2.425 53 TAGAAGGGGTGTCTGGGTG 74 19 0 57.89 58.495 3.00 0.00 2.505 54 ACCCTGTAAACCCTGGCTCC 9 20 0 60.00 62.523 3.00 2.00 2.523 55 CCCTGTAAACCCTGGCTCCT 10 20 0 60.00 62.632 3.00 0.00 2.632 56 TGAGGTCAGCACACCCTG 50 18 0 61.11 59.315 4.00 1.00 2.685 57 CCCACTGAGGTCAGCACA 45 18 0 61.11 59.315 6.00 3.00 2.685 58 CTGTAAACCCTGGCTCCTG 12 19 0 57.89 58.300 3.00 2.00 2.700 59 AGCACACCCTGCAGGTTT 57 18 0 55.56 59.261 8.00 6.00 2.739 60 CTGCACCCTGTAAACCCTGG 5 20 0 60.00 62.773 4.00 3.00 2.773 61 GTAAACCCTGGCTCCTGCCT 14 20 0 60.00 62.773 3.00 0.00 2.773 62 ACTGAGGTCAGCACACCCT 48 19 0 57.89 58.217 6.00 2.00 2.783 63 CCCTGTAAACCCTGGCTCC 10 19 0 63.16 61.789 3.00 2.00 2.789 64 GGGACACCCCACTGAGGT 38 18 0 66.67 60.812 7.00 7.00 2.812 65 TCTGCACCCTGTAAACCCT 4 19 0 52.63 58.144 4.00 0.00 2.856 66 TGCACCCTGTAAACCCTGG 6 19 0 57.89 61.900 4.00 3.00 2.900 67 CAGCACACCCTGCAGGTT 56 18 0 61.11 60.905 8.00 8.00 2.905 68 GTAAACCCTGGCTCCTGCC 14 19 0 63.16 61.937 3.00 2.00 2.937 69 GGTTTAGAAGGGGTGTCTGGG 70 21 0 57.14 61.965 3.00 0.00 2.965 70 GGACACCCCACTGAGGTCAG 39 20 0 65.00 62.981 7.00 6.00 2.981 71 GGACACCCCACTGAGGTCA 39 19 0 63.16 62.043 7.00 4.00 3.043 72 TAAACCCTGGCTCCTGCCT 15 19 0 57.89 62.055 3.00 0.00 3.055 73 CACCCTGTAAACCCTGGC 8 18 0 61.11 58.930 3.00 2.00 3.070 74 GCACCCTGTAAACCCTGG 7 18 0 61.11 58.930 3.00 3.00 3.070 75 AGCACACCCTGCAGGTTTAGA 57 21 0 52.38 62.124 8.00 4.00 3.124 76 TAAACCCTGGCTCCTGCC 15 18 0 61.11 61.147 3.00 2.00 3.147 77 GGACACCCCACTGAGGTC 39 18 0 66.67 58.806 7.00 5.00 3.194 78 CAGCACACCCTGCAGGTTT 56 19 0 57.89 62.292 8.00 6.00 3.292 79 GTCAGCACACCCTGCAGGT 54 19 0 63.16 62.397 8.00 6.00 3.397 80 TTTAGAAGGGGTGTCTGGG 72 19 0 52.63 57.569 3.00 0.00 3.431 81 GCACACCCTGCAGGTTTAGAA 58 21 0 52.38 62.477 8.00 2.00 3.477 82 AGAAGGGGTGTCTGGGTGC 75 19 0 63.16 62.484 3.00 2.00 3.484 83 AGGTCAGCACACCCTGCAG 52 19 0 63.16 62.524 6.00 6.00 3.524 84 TCAGCACACCCTGCAGGT 55 18 0 61.11 61.534 8.00 6.00 3.534 85 GTCAGCACACCCTGCAGG 54 18 0 66.67 61.534 8.00 8.00 3.534 86 GGTCAGCACACCCTGCAG 53 18 0 66.67 61.534 6.00 6.00 3.534 87 AGGTCAGCACACCCTGCA 52 18 0 61.11 61.534 4.00 4.00 3.534 88 GAAGGGGTGTCTGGGTGC 76 18 0 66.67 61.536 2.00 2.00 3.536 89 ATCTGCACCCTGTAAACCC 3 19 0 52.63 57.435 4.00 0.00 3.565 90 AGAAGGGGTGTCTGGGTG 75 18 0 61.11 58.416 3.00 0.00 3.584 91 GTAAACCCTGGCTCCTGC 14 18 0 61.11 58.230 3.00 2.00 3.770 92 CACACCCTGCAGGTTTAGA 59 19 0 52.63 57.225 8.00 3.00 3.775 93 GCACCCTGTAAACCCTGGC 7 19 0 63.16 62.779 3.00 2.00 3.779 94 GCACACCCTGCAGGTTTA 58 18 0 55.56 58.186 8.00 4.00 3.814 95 GGGACACCCCACTGAGGTC 38 19 0 68.42 62.829 7.00 5.00 3.829 96 TCAGCACACCCTGCAGGTT 55 19 0 57.89 62.920 8.00 8.00 3.920 97 CCTGTAAACCCTGGCTCC 11 18 0 61.11 58.071 3.00 2.00 3.929 98 CCCTGTAAACCCTGGCTC 10 18 0 61.11 58.071 3.00 1.00 3.929 99 TGCACCCTGTAAACCCTG 6 18 0 55.56 58.020 4.00 1.00 3.980 100 GTTTAGAAGGGGTGTCTGGGTG 71 22 0 54.55 61.999 3.00 0.00 3.999 101 CAGGTTTAGAAGGGGTGTCTGG 68 22 0 54.55 62.108 3.00 0.00 4.108 102 AAACCCTGGCTCCTGCCT 16 18 0 61.11 62.126 3.00 0.00 4.126 103 GACACCCCACTGAGGTCA 40 18 0 61.11 57.806 7.00 4.00 4.194 104 GCAGGTTTAGAAGGGGTGTCTG 67 22 0 54.55 62.245 3.00 1.00 4.245 105 TGCAGGTTTAGAAGGGGTGTCT 66 22 0 50.00 62.245 4.00 0.00 4.245 106 CTGCAGGTTTAGAAGGGGTGTC 65 22 0 54.55 62.245 6.00 1.00 4.245 107 ACCCTGGCTCCTGCCTCT 18 18 0 66.67 62.344 3.00 0.00 4.344 108 GGTTTAGAAGGGGTGTCTGGGT 70 22 0 54.55 62.650 3.00 0.00 4.650 109 AACCCTGGCTCCTGCCTC 17 18 0 66.67 62.728 3.00 0.00 4.728 110 AGGTTTAGAAGGGGTGTCTGGG 69 22 0 54.55 62.752 3.00 0.00 4.752 111 CTGAGGTCAGCACACCCT 49 18 0 61.11 57.145 6.00 2.00 4.855 112 TGTAAACCCTGGCTCCTG 13 18 0 55.56 57.142 3.00 2.00 4.858 113 ACCCTGTAAACCCTGGCT 9 18 0 55.56 57.001 3.00 3.00 4.999 primer3-2.3.6/test/primer1_list_last/included_test.for0000664000077300007730000005577012231156243022044 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGTGTCTTGTGGGTGTGTCTG 79 21 0 52.38 60.680 1.00 1.00 1.680 1 TGTCTTGTGGGTGTGTCTGTT 81 21 0 47.62 59.059 1.00 0.00 1.941 2 GTGTCTTGTGGGTGTGTCTGTT 80 22 0 50.00 59.972 1.00 0.00 2.028 3 GAACATGTGTGGCATTGTGT 21 20 0 45.00 57.775 6.00 0.00 2.225 4 GTGTCTTGTGGGTGTGTCTGT 80 21 0 52.38 58.472 1.00 0.00 2.528 5 TGTCTTGTGGGTGTGTCTGT 81 20 0 50.00 57.431 1.00 0.00 2.569 6 GTGTCTTGTGGGTGTGTCTG 80 20 0 55.00 57.431 1.00 1.00 2.569 7 TGTGTCTTGTGGGTGTGTCT 79 20 0 50.00 57.431 1.00 0.00 2.569 8 TGTCTTGTGGGTGTGTCTGTTA 81 22 0 45.45 59.108 2.00 2.00 2.892 9 TTGTGGGTGTGTCTGTTAAACA 85 22 0 40.91 58.997 7.00 7.00 3.003 10 GTGTCTTGTGGGTGTGTCTGTTA 80 23 0 47.83 59.984 2.00 2.00 3.016 11 CTTGTGGGTGTGTCTGTTAAACA 84 23 0 43.48 59.981 7.00 7.00 3.019 12 GAACATGTGTGGCATTGTGTA 21 21 0 42.86 57.880 6.00 2.00 3.120 13 TGTCTTGTGGGTGTGTCTGTTAA 81 23 0 43.48 60.493 4.00 4.00 3.493 14 TGTGGGTGTGTCTGTTAAACA 86 21 0 42.86 57.489 7.00 7.00 3.511 15 TGTGTCTTGTGGGTGTGTCTGT 79 22 0 50.00 61.549 1.00 0.00 3.549 16 AAACATGTGTGGTATTGTGCA 102 21 0 38.10 57.391 6.00 4.00 3.609 17 TTGTGGGTGTGTCTGTTAAACAT 85 23 0 39.13 59.319 7.00 5.00 3.681 18 GAACATGTGTGGCATTGTGTAT 21 22 0 40.91 58.283 6.00 2.00 3.717 19 TGGGTGTGTCTGTTAAACATGTG 88 23 0 43.48 60.746 7.00 4.00 3.746 20 TGTGGGTGTGTCTGTTAAACATG 86 23 0 43.48 60.746 7.00 5.00 3.746 21 TCTTGTGGGTGTGTCTGTTAAA 83 22 0 40.91 58.187 4.00 3.00 3.813 22 AGCTTCTTGACTGTGTGTTGTG 149 22 0 45.45 58.075 4.00 0.00 3.925 23 TCTTGTGGGTGTGTCTGTTAAAC 83 23 0 43.48 59.063 5.00 5.00 3.937 24 GTCTTGTGGGTGTGTCTGTTAAA 82 23 0 43.48 59.063 4.00 3.00 3.937 25 TGGATGTAGCTTCTTGACTGTG 142 22 0 45.45 58.036 4.00 1.00 3.964 26 AGCTTCTTGACTGTGTGTTGTGT 149 23 0 43.48 58.981 4.00 0.00 4.019 27 GGATGTAGCTTCTTGACTGTGTG 143 23 0 47.83 58.925 4.00 0.00 4.075 28 TGGATGTAGCTTCTTGACTGTGT 142 23 0 43.48 58.925 4.00 1.00 4.075 29 TTAAACATGTGTGGTATTGTGCA 100 23 0 34.78 58.925 6.00 4.00 4.075 30 GGGTGTGTCTGTTAAACATGTG 89 22 0 45.45 57.912 7.00 4.00 4.088 31 TGGGTGTGTCTGTTAAACATGT 88 22 0 40.91 57.912 7.00 6.00 4.088 32 GTGGGTGTGTCTGTTAAACATG 87 22 0 45.45 57.912 7.00 5.00 4.088 33 TGTGGGTGTGTCTGTTAAACAT 86 22 0 40.91 57.912 7.00 5.00 4.088 34 AACATGTGTGGCATTGTGTATACA 22 24 0 37.50 60.103 8.00 8.00 4.103 35 GTCTTGTGGGTGTGTCTGTTAAAC 82 24 0 45.83 59.875 5.00 5.00 4.125 36 CATGTGTGGCATTGTGTATACA 24 22 0 40.91 57.850 8.00 8.00 4.150 37 AAACATGTGTGGTATTGTGCAT 102 22 0 36.36 57.811 6.00 2.00 4.189 38 GGGTGTGTCTGTTAAACATGTGT 89 23 0 43.48 58.808 7.00 6.00 4.192 39 GTGGGTGTGTCTGTTAAACATGT 87 23 0 43.48 58.808 7.00 6.00 4.192 40 ACATGTGTGGCATTGTGTATACA 23 23 0 39.13 58.766 8.00 8.00 4.234 41 CTTGTGGGTGTGTCTGTTAAACAT 84 24 0 41.67 60.239 7.00 5.00 4.239 42 GGATGTAGCTTCTTGACTGTGTGT 143 24 0 45.83 59.748 4.00 0.00 4.252 43 GTTAAACATGTGTGGTATTGTGCA 99 24 0 37.50 59.733 6.00 4.00 4.267 44 TGTTAAACATGTGTGGTATTGTGC 98 24 0 37.50 59.733 7.00 2.00 4.267 45 GTCTTGTGGGTGTGTCTGTTAA 82 22 0 45.45 57.635 4.00 4.00 4.365 46 TAAACATGTGTGGTATTGTGCA 101 22 0 36.36 57.504 6.00 4.00 4.496 47 TGTGTGTTGTGTATGGATGTAGC 129 23 0 43.48 58.499 2.00 2.00 4.501 48 TGTGTGTTGTGTATGGATGTAGCT 129 24 0 41.67 59.493 4.00 4.00 4.507 49 ATGGATGTAGCTTCTTGACTGTG 141 23 0 43.48 58.408 4.00 1.00 4.592 50 GAACATGTGTGGCATTGTGTATA 21 23 0 39.13 58.360 6.00 4.00 4.640 51 TTGTGCATATACATGTGTGTTGTG 116 24 0 37.50 59.345 6.00 2.00 4.655 52 ATGGATGTAGCTTCTTGACTGTGT 141 24 0 41.67 59.238 4.00 1.00 4.762 53 TTAAACATGTGTGGTATTGTGCAT 100 24 0 33.33 59.233 6.00 2.00 4.767 54 GAACATGTGTGGCATTGTGTATAC 21 24 0 41.67 59.208 6.00 6.00 4.792 55 CTTGTGGGTGTGTCTGTTAAAC 84 22 0 45.45 57.176 5.00 5.00 4.824 56 TAGCTTCTTGACTGTGTGTTGTG 148 23 0 43.48 58.160 4.00 0.00 4.840 57 TGTAGCTTCTTGACTGTGTGTTG 146 23 0 43.48 58.160 4.00 0.00 4.840 58 TGTGTTGTGTATGGATGTAGCTTC 131 24 0 41.67 59.091 4.00 4.00 4.909 59 TAGCTTCTTGACTGTGTGTTGTGT 148 24 0 41.67 59.030 4.00 0.00 4.970 60 GTAGCTTCTTGACTGTGTGTTGTG 147 24 0 45.83 59.030 4.00 0.00 4.970 61 TGTAGCTTCTTGACTGTGTGTTGT 146 24 0 41.67 59.030 4.00 0.00 4.970 62 TGTGTCTGTTAAACATGTGTGG 92 22 0 40.91 57.024 7.00 2.00 4.976 63 TGTGTTGTGTATGGATGTAGCTTCT 131 25 0 40.00 60.002 4.00 2.00 5.002 64 GTTAAACATGTGTGGTATTGTGCAT 99 25 0 36.00 59.991 6.00 2.00 5.009 65 TGTGTCTGTTAAACATGTGTGGT 92 23 0 39.13 57.983 7.00 0.00 5.017 66 GTGTGTCTGTTAAACATGTGTGG 91 23 0 43.48 57.983 7.00 2.00 5.017 67 GGTGTGTCTGTTAAACATGTGTG 90 23 0 43.48 57.983 7.00 4.00 5.017 68 TGTGCATATACATGTGTGTTGTG 117 23 0 39.13 57.923 6.00 2.00 5.077 69 AAACATGTGTGGTATTGTGCATA 102 23 0 34.78 57.903 6.00 4.00 5.097 70 TAAACATGTGTGGTATTGTGCAT 101 23 0 34.78 57.903 6.00 2.00 5.097 71 GTGTGTTGTGTATGGATGTAGCTTC 130 25 0 44.00 59.880 4.00 4.00 5.120 72 GTGTGTCTGTTAAACATGTGTGGT 91 24 0 41.67 58.868 7.00 0.00 5.132 73 TTGTGCATATACATGTGTGTTGTGT 116 25 0 36.00 60.151 6.00 0.00 5.151 74 ATGTGTGTTGTGTATGGATGTAGC 128 24 0 41.67 58.842 2.00 2.00 5.158 75 GTAGCTTCTTGACTGTGTGTTGTGT 147 25 0 44.00 59.835 4.00 0.00 5.165 76 TTGTGTATGGATGTAGCTTCTTGA 135 24 0 37.50 58.834 4.00 2.00 5.166 77 TGTGCATATACATGTGTGTTGTGT 117 24 0 37.50 58.828 6.00 0.00 5.172 78 ATGTGTGTTGTGTATGGATGTAGCT 128 25 0 40.00 59.772 4.00 4.00 5.228 79 GTGTCTTGTGGGTGTGTCTGTTAA 80 24 0 45.83 61.270 4.00 4.00 5.270 80 GATGTAGCTTCTTGACTGTGTGTTG 144 25 0 44.00 60.272 4.00 0.00 5.272 81 ACATGTGTGTTGTGTATGGATGT 126 23 0 39.13 57.646 6.00 0.00 5.354 82 ATTGTGCATATACATGTGTGTTGTG 115 25 0 36.00 59.637 6.00 2.00 5.363 83 TTGTGTATGGATGTAGCTTCTTGAC 135 25 0 40.00 59.615 4.00 1.00 5.385 84 GTTGTGTATGGATGTAGCTTCTTGA 134 25 0 40.00 59.615 4.00 2.00 5.385 85 TGTTGTGTATGGATGTAGCTTCTTG 133 25 0 40.00 60.458 4.00 2.00 5.458 86 ATGTAGCTTCTTGACTGTGTGTTG 145 24 0 41.67 58.520 4.00 0.00 5.480 87 GGTGTGTCTGTTAAACATGTGTGG 90 24 0 45.83 61.509 7.00 2.00 5.509 88 GGGTGTGTCTGTTAAACATGTGTG 89 24 0 45.83 61.509 7.00 4.00 5.509 89 TGGGTGTGTCTGTTAAACATGTGT 88 24 0 41.67 61.509 7.00 6.00 5.509 90 GTGGGTGTGTCTGTTAAACATGTG 87 24 0 45.83 61.509 7.00 4.00 5.509 91 TGTGGGTGTGTCTGTTAAACATGT 86 24 0 41.67 61.509 7.00 6.00 5.509 92 TATGGATGTAGCTTCTTGACTGTG 140 24 0 41.67 58.476 4.00 1.00 5.524 93 TGTATGGATGTAGCTTCTTGACTG 138 24 0 41.67 58.476 4.00 1.00 5.524 94 TGTGTATGGATGTAGCTTCTTGA 136 23 0 39.13 57.467 4.00 2.00 5.533 95 CTGTTAAACATGTGTGGTATTGTGC 97 25 0 40.00 60.599 7.00 2.00 5.599 96 TGGATGTAGCTTCTTGACTGTGTG 142 24 0 45.83 61.619 4.00 0.00 5.619 97 AACATGTGTGGCATTGTGTATAC 22 23 0 39.13 57.373 6.00 6.00 5.627 98 TGTGTATGGATGTAGCTTCTTGAC 136 24 0 41.67 58.339 4.00 1.00 5.661 99 ATGTAGCTTCTTGACTGTGTGTTGT 145 25 0 40.00 59.333 4.00 0.00 5.667 100 TGGTATTGTGCATATACATGTGTG 111 24 0 37.50 58.315 6.00 4.00 5.685 101 TGTGGTATTGTGCATATACATGTG 109 24 0 37.50 58.315 6.00 4.00 5.685 102 TGTGTGGTATTGTGCATATACATG 107 24 0 37.50 58.315 6.00 6.00 5.685 103 CATGTGTGGTATTGTGCATATACA 105 24 0 37.50 58.315 6.00 6.00 5.685 104 TATGGATGTAGCTTCTTGACTGTGT 140 25 0 40.00 59.274 4.00 1.00 5.726 105 GTATGGATGTAGCTTCTTGACTGTG 139 25 0 44.00 59.274 4.00 1.00 5.726 106 TGTATGGATGTAGCTTCTTGACTGT 138 25 0 40.00 59.274 4.00 3.00 5.726 107 GTGTATGGATGTAGCTTCTTGACTG 137 25 0 44.00 59.274 4.00 1.00 5.726 108 TGTGTATGGATGTAGCTTCTTGACT 136 25 0 40.00 59.274 4.00 1.00 5.726 109 AGCTTCTTGACTGTGTGTTGTGTG 149 24 0 45.83 61.730 4.00 0.00 5.730 110 TTAAACATGTGTGGTATTGTGCATA 100 25 0 32.00 59.268 6.00 4.00 5.732 111 TCTTGTGGGTGTGTCTGTTAAACA 83 24 0 41.67 61.733 7.00 7.00 5.733 112 TGTCTTGTGGGTGTGTCTGTTAAA 81 24 0 41.67 61.733 4.00 3.00 5.733 113 AAACATGTGTGGTATTGTGCATAT 102 24 0 33.33 58.262 6.00 4.00 5.738 114 TGTGTGTTGTGTATGGATGTAGCTT 129 25 0 40.00 60.742 4.00 2.00 5.742 115 TGTGTTGTGTATGGATGTAGCTT 131 23 0 39.13 57.257 4.00 2.00 5.743 116 TGTGTCTTGTGGGTGTGTCTGTT 79 23 0 47.83 62.831 1.00 0.00 5.831 117 GTGTGTTGTGTATGGATGTAGCTT 130 24 0 41.67 58.137 4.00 2.00 5.863 118 GCATATACATGTGTGTTGTGTATGG 120 25 0 40.00 59.136 6.00 3.00 5.864 119 TGGTATTGTGCATATACATGTGTGT 111 25 0 36.00 59.136 6.00 6.00 5.864 120 GTGGTATTGTGCATATACATGTGTG 110 25 0 40.00 59.136 6.00 4.00 5.864 121 TGTGGTATTGTGCATATACATGTGT 109 25 0 36.00 59.136 6.00 6.00 5.864 122 GTGTGGTATTGTGCATATACATGTG 108 25 0 40.00 59.136 6.00 4.00 5.864 123 TGTGTGGTATTGTGCATATACATGT 107 25 0 36.00 59.136 6.00 6.00 5.864 124 ACATGTGTGGTATTGTGCATATACA 104 25 0 36.00 59.136 6.00 6.00 5.864 125 TGTGTCTGTTAAACATGTGTGGTA 92 24 0 37.50 58.069 7.00 2.00 5.931 126 GTTAAACATGTGTGGTATTGTGC 99 23 0 39.13 57.060 6.00 2.00 5.940 127 GGATGTAGCTTCTTGACTGTGTGTT 143 25 0 44.00 60.962 4.00 0.00 5.962 128 TTGTGTATGGATGTAGCTTCTTG 135 23 0 39.13 57.036 4.00 2.00 5.964 129 TTGTGGGTGTGTCTGTTAAACATG 85 24 0 41.67 61.967 7.00 5.00 5.967 130 GTTAAACATGTGTGGTATTGTGCATA 99 26 0 34.62 60.001 6.00 4.00 6.001 131 TAAACATGTGTGGTATTGTGCATA 101 24 0 33.33 57.988 6.00 4.00 6.012 132 GTATGGATGTAGCTTCTTGACTGTGT 139 26 0 42.31 60.018 4.00 1.00 6.018 133 GTGTATGGATGTAGCTTCTTGACTGT 137 26 0 42.31 60.018 4.00 3.00 6.018 134 GTGTGTCTGTTAAACATGTGTGGTA 91 25 0 40.00 58.919 7.00 2.00 6.081 135 TCTGTTAAACATGTGTGGTATTGTG 96 25 0 36.00 58.912 7.00 0.00 6.088 136 TGTCTGTTAAACATGTGTGGTATTG 94 25 0 36.00 58.912 7.00 0.00 6.088 137 GTTGTGTATGGATGTAGCTTCTTG 134 24 0 41.67 57.904 4.00 2.00 6.096 138 TGTTGTGTATGGATGTAGCTTCTT 133 24 0 37.50 57.904 4.00 4.00 6.096 139 GTGGTATTGTGCATATACATGTGTGT 110 26 0 38.46 59.900 6.00 6.00 6.100 140 GTGTGGTATTGTGCATATACATGTGT 108 26 0 38.46 59.900 6.00 6.00 6.100 141 CATGTGTGTTGTGTATGGATGTAG 127 24 0 41.67 57.896 4.00 0.00 6.104 142 TGTGCATATACATGTGTGTTGTGTA 117 25 0 36.00 58.882 6.00 2.00 6.118 143 TTGTGCATATACATGTGTGTTGTGTA 116 26 0 34.62 60.156 6.00 2.00 6.156 144 ACATGTGTGTTGTGTATGGATGTAG 126 25 0 40.00 58.761 6.00 0.00 6.239 145 ACATGTGTGTTGTGTATGGATGTA 126 24 0 37.50 57.747 6.00 2.00 6.253 146 TACATGTGTGTTGTGTATGGATGT 125 24 0 37.50 57.747 6.00 0.00 6.253 147 GTGTTGTGTATGGATGTAGCTTCTT 132 25 0 40.00 58.712 4.00 4.00 6.288 148 GTCTGTTAAACATGTGTGGTATTGTG 95 26 0 38.46 59.680 7.00 0.00 6.320 149 TGTCTGTTAAACATGTGTGGTATTGT 94 26 0 34.62 59.680 7.00 0.00 6.320 150 GTGTCTGTTAAACATGTGTGGTATTG 93 26 0 38.46 59.680 7.00 0.00 6.320 151 TGTGTCTGTTAAACATGTGTGGTATT 92 26 0 34.62 59.680 7.00 1.00 6.320 152 TATTGTGCATATACATGTGTGTTGTG 114 26 0 34.62 59.659 6.00 2.00 6.341 153 GTTGTGTATGGATGTAGCTTCTTGAC 134 26 0 42.31 60.343 4.00 1.00 6.343 154 GGTATTGTGCATATACATGTGTGTTG 112 26 0 38.46 60.346 6.00 4.00 6.346 155 TGGTATTGTGCATATACATGTGTGTT 111 26 0 34.62 60.346 6.00 4.00 6.346 156 AACATGTGTGGTATTGTGCATATACA 103 26 0 34.62 60.346 6.00 6.00 6.346 157 ATGTGTGGTATTGTGCATATACATG 106 25 0 36.00 58.650 6.00 6.00 6.350 158 CATGTGTGGTATTGTGCATATACAT 105 25 0 36.00 58.650 6.00 4.00 6.350 159 GATGTAGCTTCTTGACTGTGTGTT 144 24 0 41.67 57.626 4.00 0.00 6.374 160 ATTGTGCATATACATGTGTGTTGTGT 115 26 0 34.62 60.393 6.00 0.00 6.393 161 TTGTGTATGGATGTAGCTTCTTGACT 135 26 0 38.46 60.455 4.00 1.00 6.455 162 TTAAACATGTGTGGTATTGTGCATAT 100 26 0 30.77 59.536 6.00 4.00 6.464 163 CATGTGTGTTGTGTATGGATGTAGC 127 25 0 44.00 61.472 4.00 2.00 6.472 164 TGTGTCTGTTAAACATGTGTGGTAT 92 25 0 36.00 58.424 7.00 2.00 6.576 165 ATGTGTGGTATTGTGCATATACATGT 106 26 0 34.62 59.420 6.00 6.00 6.580 166 ACATGTGTGGTATTGTGCATATACAT 104 26 0 34.62 59.420 6.00 4.00 6.580 167 GTGTTGTGTATGGATGTAGCTTCT 132 24 0 41.67 57.400 4.00 2.00 6.600 168 TGCATATACATGTGTGTTGTGTATG 119 25 0 36.00 58.378 6.00 5.00 6.622 169 AAACATGTGTGGTATTGTGCATATA 102 25 0 32.00 58.331 6.00 4.00 6.669 170 TAAACATGTGTGGTATTGTGCATAT 101 25 0 32.00 58.331 6.00 4.00 6.669 171 TAGCTTCTTGACTGTGTGTTGTGTG 148 25 0 44.00 61.678 4.00 0.00 6.678 172 TGTAGCTTCTTGACTGTGTGTTGTG 146 25 0 44.00 61.678 4.00 0.00 6.678 173 GTGTGTTGTGTATGGATGTAGCTTCT 130 26 0 42.31 60.727 4.00 2.00 6.727 174 TGTGTCTTGTGGGTGTGTCTGTTA 79 24 0 45.83 62.734 2.00 2.00 6.734 175 ATACATGTGTGTTGTGTATGGATG 124 24 0 37.50 57.241 6.00 0.00 6.759 176 GTGTGTCTGTTAAACATGTGTGGTAT 91 26 0 38.46 59.220 7.00 2.00 6.780 177 ATGGATGTAGCTTCTTGACTGTGTG 141 25 0 44.00 61.791 4.00 0.00 6.791 178 GAACATGTGTGGCATTGTGTATACA 21 25 0 40.00 61.810 8.00 8.00 6.810 179 GTGCATATACATGTGTGTTGTGTATG 118 26 0 38.46 59.190 6.00 5.00 6.810 180 TGTGCATATACATGTGTGTTGTGTAT 117 26 0 34.62 59.190 6.00 3.00 6.810 181 GCATATACATGTGTGTTGTGTATGGA 120 26 0 38.46 60.811 6.00 1.00 6.811 182 CTGTTAAACATGTGTGGTATTGTG 97 24 0 37.50 57.140 7.00 0.00 6.860 183 ATACATGTGTGTTGTGTATGGATGT 124 25 0 36.00 58.124 6.00 0.00 6.876 184 TCTTGTGGGTGTGTCTGTTAAACAT 83 25 0 40.00 61.898 7.00 5.00 6.898 185 AAACATGTGTGGTATTGTGCATATAC 102 26 0 34.62 59.095 6.00 6.00 6.905 186 ATGTGTGTTGTGTATGGATGTAGCTT 128 26 0 38.46 60.948 4.00 2.00 6.948 187 AACATGTGTGGTATTGTGCATATA 103 24 0 33.33 57.002 6.00 4.00 6.998 188 GATGTAGCTTCTTGACTGTGTGTTGT 144 26 0 42.31 61.001 4.00 0.00 7.001 189 CATGTGTGGTATTGTGCATATACATG 105 26 0 38.46 61.147 6.00 6.00 7.147 190 GGTATTGTGCATATACATGTGTGTT 112 25 0 36.00 57.851 6.00 4.00 7.149 191 AACATGTGTGGTATTGTGCATATAC 103 25 0 36.00 57.851 6.00 6.00 7.149 192 GTGTTGTGTATGGATGTAGCTTCTTG 132 26 0 42.31 61.157 4.00 2.00 7.157 193 TGTGTTGTGTATGGATGTAGCTTCTT 131 26 0 38.46 61.157 4.00 4.00 7.157 194 TACATGTGTGTTGTGTATGGATGTA 125 25 0 36.00 57.841 6.00 2.00 7.159 195 TACATGTGTGTTGTGTATGGATGTAG 125 26 0 38.46 58.815 6.00 0.00 7.185 196 GGTGTGTCTGTTAAACATGTGTGGT 90 25 0 44.00 62.217 7.00 0.00 7.217 197 GTGGGTGTGTCTGTTAAACATGTGT 87 25 0 44.00 62.217 7.00 6.00 7.217 198 GTTAAACATGTGTGGTATTGTGCATAT 99 27 0 33.33 60.227 6.00 4.00 7.227 199 CATATACATGTGTGTTGTGTATGGATG 121 27 0 37.04 60.230 6.00 0.00 7.230 200 TGTTAAACATGTGTGGTATTGTGCA 98 25 0 36.00 62.264 7.00 4.00 7.264 201 TGGATGTAGCTTCTTGACTGTGTGT 142 25 0 44.00 62.322 4.00 0.00 7.322 202 TTGTGCATATACATGTGTGTTGTGTAT 116 27 0 33.33 60.389 6.00 3.00 7.389 203 ATTGTGCATATACATGTGTGTTGTGTA 115 27 0 33.33 60.389 6.00 2.00 7.389 204 TATTGTGCATATACATGTGTGTTGTGT 114 27 0 33.33 60.389 6.00 0.00 7.389 205 GTATTGTGCATATACATGTGTGTTGTG 113 27 0 37.04 60.389 6.00 2.00 7.389 206 GTGTCTGTTAAACATGTGTGGTATTGT 93 27 0 37.04 60.396 7.00 0.00 7.396 207 GTGTGTCTGTTAAACATGTGTGGTATT 91 27 0 37.04 60.396 7.00 1.00 7.396 208 TGTTGTGTATAAATGTTGTGTCTTCA 52 26 0 30.77 58.596 4.00 1.00 7.404 209 GTCTTGTGGGTGTGTCTGTTAAACA 82 25 0 44.00 62.427 7.00 7.00 7.427 210 TGTCTTGTGGGTGTGTCTGTTAAAC 81 25 0 44.00 62.427 5.00 5.00 7.427 211 GTGTCTTGTGGGTGTGTCTGTTAAA 80 25 0 44.00 62.427 4.00 3.00 7.427 212 TTAAACATGTGTGGTATTGTGCATATA 100 27 0 29.63 59.560 6.00 4.00 7.440 213 AGCTTCTTGACTGTGTGTTGTGTGT 149 25 0 44.00 62.443 4.00 0.00 7.443 214 AACATGTGTGGTATTGTGCATATACAT 103 27 0 33.33 60.562 6.00 4.00 7.562 215 TAAACATGTGTGGTATTGTGCATATA 101 26 0 30.77 58.395 6.00 4.00 7.605 216 GTGTTGTGTATAAATGTTGTGTCTTCA 51 27 0 33.33 59.358 4.00 1.00 7.642 217 TGTGTTGTGTATAAATGTTGTGTCTTC 50 27 0 33.33 59.358 4.00 0.00 7.642 218 TTGTGGGTGTGTCTGTTAAACATGT 85 25 0 40.00 62.648 7.00 6.00 7.648 219 TATACATGTGTGTTGTGTATGGATG 123 25 0 36.00 57.352 6.00 0.00 7.648 220 CATATACATGTGTGTTGTGTATGGA 121 25 0 36.00 57.352 6.00 0.00 7.648 221 TGCATATACATGTGTGTTGTGTATGG 119 26 0 38.46 61.654 6.00 3.00 7.654 222 TGTGGTATTGTGCATATACATGTGTG 109 26 0 38.46 61.654 6.00 4.00 7.654 223 TGTGTGGTATTGTGCATATACATGTG 107 26 0 38.46 61.654 6.00 4.00 7.654 224 TATGGATGTAGCTTCTTGACTGTGTG 140 26 0 42.31 61.740 4.00 0.00 7.740 225 TGTATGGATGTAGCTTCTTGACTGTG 138 26 0 42.31 61.740 4.00 1.00 7.740 226 TGTGTATGGATGTAGCTTCTTGACTG 136 26 0 42.31 61.740 4.00 1.00 7.740 227 CTTGTGGGTGTGTCTGTTAAACATG 84 25 0 44.00 62.749 7.00 5.00 7.749 228 ATACATGTGTGTTGTGTATGGATGTA 124 26 0 34.62 58.200 6.00 2.00 7.800 229 TATACATGTGTGTTGTGTATGGATGT 123 26 0 34.62 58.200 6.00 0.00 7.800 230 ATGTAGCTTCTTGACTGTGTGTTGTG 145 26 0 42.31 61.844 4.00 0.00 7.844 231 GTCTGTTAAACATGTGTGGTATTGT 95 25 0 36.00 57.143 7.00 0.00 7.857 232 GTGTCTGTTAAACATGTGTGGTATT 93 25 0 36.00 57.143 7.00 1.00 7.857 233 TAAACATGTGTGGTATTGTGCATATAC 101 27 0 33.33 59.133 6.00 6.00 7.867 234 ATACATGTGTGTTGTGTATGGATGTAG 124 27 0 37.04 59.113 6.00 0.00 7.887 235 TATTGTGCATATACATGTGTGTTGT 114 25 0 32.00 57.073 6.00 2.00 7.927 236 GTATTGTGCATATACATGTGTGTTG 113 25 0 36.00 57.073 6.00 4.00 7.927 237 GCATATACATGTGTGTTGTGTATGGAT 120 27 0 37.04 61.010 6.00 2.00 8.010 238 GGTATTGTGCATATACATGTGTGTTGT 112 27 0 37.04 61.034 6.00 2.00 8.034 239 GTGGTATTGTGCATATACATGTGTGTT 110 27 0 37.04 61.034 6.00 4.00 8.034 240 TGTTGTGTATGGATGTAGCTTCTTGA 133 26 0 38.46 62.056 4.00 2.00 8.056 241 GTATTGTGCATATACATGTGTGTTGT 113 26 0 34.62 57.913 6.00 2.00 8.087 242 GTTGTGTATGGATGTAGCTTCTTGACT 134 27 0 40.74 61.126 4.00 1.00 8.126 243 GGTGTGTCTGTTAAACATGTGTGGTA 90 26 0 42.31 62.150 7.00 2.00 8.150 244 ACATGTGTGTTGTGTATGGATGTAGC 126 26 0 42.31 62.163 6.00 2.00 8.163 245 TCTGTTAAACATGTGTGGTATTGTGC 96 26 0 38.46 62.185 7.00 2.00 8.185 246 CATGTGTGTTGTGTATGGATGTAGCT 127 26 0 42.31 62.266 4.00 4.00 8.266 247 ATATACATGTGTGTTGTGTATGGATG 122 26 0 34.62 57.730 6.00 0.00 8.270 248 CATATACATGTGTGTTGTGTATGGAT 121 26 0 34.62 57.730 6.00 2.00 8.270 249 TGTGTTGTGTATAAATGTTGTGTCTT 50 26 0 30.77 57.700 4.00 0.00 8.300 250 TAGCTTCTTGACTGTGTGTTGTGTGT 148 26 0 42.31 62.367 4.00 0.00 8.367 251 GTAGCTTCTTGACTGTGTGTTGTGTG 147 26 0 46.15 62.367 4.00 0.00 8.367 252 TGTAGCTTCTTGACTGTGTGTTGTGT 146 26 0 42.31 62.367 4.00 0.00 8.367 253 TGTGTGTTGTGTATGGATGTAGCTTC 129 26 0 42.31 62.368 4.00 4.00 8.368 254 TGTTAAACATGTGTGGTATTGTGCAT 98 26 0 34.62 62.397 7.00 2.00 8.397 255 AAACATGTGTGGTATTGTGCATATACA 102 27 0 33.33 61.445 6.00 6.00 8.445 256 ATGGATGTAGCTTCTTGACTGTGTGT 141 26 0 42.31 62.454 4.00 0.00 8.454 257 ATATACATGTGTGTTGTGTATGGATGT 122 27 0 33.33 58.526 6.00 0.00 8.474 258 GTCTTGTGGGTGTGTCTGTTAAACAT 82 26 0 42.31 62.553 7.00 5.00 8.553 259 TGTGCATATACATGTGTGTTGTGTATG 117 27 0 37.04 61.688 6.00 5.00 8.688 260 TATACATGTGTGTTGTGTATGGATGTA 123 27 0 33.33 58.271 6.00 2.00 8.729 261 GTGTGTTGTGTATGGATGTAGCTTCTT 130 27 0 40.74 61.811 4.00 4.00 8.811 262 ATGTGTGGTATTGTGCATATACATGTG 106 27 0 37.04 61.814 6.00 4.00 8.814 263 CATGTGTGGTATTGTGCATATACATGT 105 27 0 37.04 61.814 6.00 6.00 8.814 264 ACATGTGTGGTATTGTGCATATACATG 104 27 0 37.04 61.814 6.00 6.00 8.814 265 ATGTGTTGTGTATAAATGTTGTGTCTT 49 27 0 29.63 58.041 4.00 0.00 8.959 266 TGTCTGTTAAACATGTGTGGTATTGTG 94 27 0 37.04 62.089 7.00 0.00 9.089 267 TGTGTCTGTTAAACATGTGTGGTATTG 92 27 0 37.04 62.089 7.00 0.00 9.089 268 TACATGTGTGTTGTGTATGGATGTAGC 125 27 0 40.74 62.100 6.00 2.00 9.100 269 GGTGTGTCTGTTAAACATGTGTGGTAT 90 27 0 40.74 62.286 7.00 2.00 9.286 270 GTGCATATACATGTGTGTTGTGTATGG 118 27 0 40.74 62.312 6.00 3.00 9.312 271 TGTGGTATTGTGCATATACATGTGTGT 109 27 0 37.04 62.312 6.00 6.00 9.312 272 GTGTGGTATTGTGCATATACATGTGTG 108 27 0 40.74 62.312 6.00 4.00 9.312 273 TGTGTGGTATTGTGCATATACATGTGT 107 27 0 37.04 62.312 6.00 6.00 9.312 274 TGTTAAACATGTGTGGTATTGTGCATA 98 27 0 33.33 62.329 7.00 4.00 9.329 275 TATGGATGTAGCTTCTTGACTGTGTGT 140 27 0 40.74 62.383 4.00 0.00 9.383 276 GTATGGATGTAGCTTCTTGACTGTGTG 139 27 0 44.44 62.383 4.00 0.00 9.383 277 TGTATGGATGTAGCTTCTTGACTGTGT 138 27 0 40.74 62.383 4.00 1.00 9.383 278 GTGTATGGATGTAGCTTCTTGACTGTG 137 27 0 44.44 62.383 4.00 1.00 9.383 279 TGTGTATGGATGTAGCTTCTTGACTGT 136 27 0 40.74 62.383 4.00 3.00 9.383 280 ATGTGTGTTGTGTATGGATGTAGCTTC 128 27 0 40.74 62.494 4.00 4.00 9.494 281 ATGTAGCTTCTTGACTGTGTGTTGTGT 145 27 0 40.74 62.495 4.00 0.00 9.495 282 TGTTGTGTATGGATGTAGCTTCTTGAC 133 27 0 40.74 62.685 4.00 1.00 9.685 283 GTGTTGTGTATGGATGTAGCTTCTTGA 132 27 0 40.74 62.685 4.00 2.00 9.685 284 TGGTATTGTGCATATACATGTGTGTTG 111 27 0 37.04 62.716 6.00 4.00 9.716 285 TTGTGTATGGATGTAGCTTCTTGACTG 135 27 0 40.74 62.778 4.00 1.00 9.778 286 GTCTGTTAAACATGTGTGGTATTGTGC 95 27 0 40.74 62.806 7.00 2.00 9.806 287 CATATGTGTTGTGTATAAATGTTGTGT 46 27 0 29.63 57.112 6.00 0.00 9.888 288 ACATATGTGTTGTGTATAAATGTTGTG 45 27 0 29.63 57.112 8.00 0.00 9.888 289 CACATATGTGTTGTGTATAAATGTTGT 44 27 0 29.63 57.112 10.00 2.00 9.888 290 ACACATATGTGTTGTGTATAAATGTTG 43 27 0 29.63 57.112 12.00 0.00 9.888 291 ACATGTGTGTTGTGTATGGATGTAGCT 126 27 0 40.74 62.902 6.00 4.00 9.902 primer3-2.3.6/test/primer1_list_last/long_seq.for0000664000077300007730000022057012231156243021015 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 gcgatcgatgctagctagct 373 20 0 55.00 59.880 10.00 8.00 0.120 1 gatcgatgcatgcatgctac 446 20 0 50.00 60.222 10.00 8.00 0.222 2 gatgctagctaggcgatgct 409 20 0 55.00 59.742 8.00 3.00 0.258 3 cggcgatctagctagctgac 582 20 0 60.00 60.278 8.00 6.00 0.278 4 cgtagcggcgatctagctag 577 20 0 60.00 60.285 8.00 8.00 0.285 5 tagcggcgatctagctagct 579 20 0 55.00 59.523 8.00 8.00 0.477 6 atcgatgtgcggctagctag 86 20 0 55.00 60.531 8.00 8.00 0.531 7 tatcatcgatcgatgcatgc 439 20 0 45.00 60.574 12.00 8.00 0.574 8 gtagcggcgatctagctagc 578 20 0 60.00 59.393 8.00 6.00 0.607 9 gatcgatgctagctaggcga 405 20 0 55.00 60.615 8.00 4.00 0.615 10 tcgatcgatgctagctaggc 403 20 0 55.00 60.615 8.00 6.00 0.615 11 gctagctgactgatacgcga 468 20 0 55.00 59.351 6.00 2.00 0.649 12 atcgatgcatgcatgctact 447 20 0 45.00 59.285 10.00 7.00 0.715 13 atcgatgctacatcgatcga 315 20 0 45.00 59.209 12.00 8.00 0.791 14 atcgatgctagctaggcgat 406 20 0 50.00 59.074 8.00 6.00 0.926 15 tcgatgcatgcatgctacta 448 20 0 45.00 58.989 10.00 8.00 1.011 16 tcgatgcatgcatgctactag 448 21 0 47.62 60.014 10.00 7.00 1.014 17 gctagctgatcgatcgatgc 394 20 0 55.00 61.025 10.00 8.00 1.025 18 gactgatcgatcgatgctagc 116 21 0 52.38 59.961 10.00 6.00 1.039 19 ctgactgatcgatcgatgct 114 20 0 50.00 58.936 10.00 6.00 1.064 20 agctgactgatcgatcgatg 112 20 0 50.00 58.936 10.00 8.00 1.064 21 gctagctagctgatcgatcga 390 21 0 52.38 59.866 10.00 8.00 1.134 22 gctagctagctgatcgatcga 70 21 0 52.38 59.866 10.00 8.00 1.134 23 tcgtagcggcgatctagcta 576 20 0 55.00 61.150 6.00 6.00 1.150 24 cgcgatcgatgctagctag 372 19 0 57.89 59.848 8.00 8.00 1.152 25 gctactatcatctctgcgcg 356 20 0 55.00 58.801 4.00 4.00 1.199 26 ctagctgatcgatcgtagcg 564 20 0 55.00 58.799 8.00 4.00 1.201 27 agctactatcatctctgcgcg 355 21 0 52.38 59.789 4.00 4.00 1.211 28 gatgctagctaggcgatgcta 409 21 0 52.38 59.761 8.00 5.00 1.239 29 agctgactagctgatcgatcg 595 21 0 52.38 59.755 8.00 6.00 1.245 30 agctagctgactgatcgatcg 108 21 0 52.38 59.755 8.00 6.00 1.245 31 tcgatgtgcggctagctag 87 19 0 57.89 60.272 8.00 8.00 1.272 32 gctgactagctgatcgatcg 596 20 0 55.00 58.726 8.00 6.00 1.274 33 gctagctgactgatcgatcg 109 20 0 55.00 58.726 8.00 6.00 1.274 34 cgatgctagctaggcgatg 408 19 0 57.89 59.703 8.00 4.00 1.297 35 gtagcggcgatctagctagct 578 21 0 57.14 60.297 8.00 8.00 1.297 36 agctagctgactgatacgcga 467 21 0 52.38 60.328 8.00 2.00 1.328 37 gactgatacgcgatgctagct 475 21 0 52.38 59.657 6.00 4.00 1.343 38 gctagctgactgatacgcgat 468 21 0 52.38 59.657 6.00 4.00 1.343 39 gactgatacgcgatgctagc 475 20 0 55.00 58.655 6.00 6.00 1.345 40 gctgactagctgatcgatcgt 596 21 0 52.38 59.618 8.00 4.00 1.382 41 atcgtagcggcgatctagct 575 20 0 55.00 61.387 6.00 4.00 1.387 42 tgctagctaggcgatgctag 411 20 0 55.00 58.609 8.00 7.00 1.391 43 tagctgatcgatcgtagcgg 565 20 0 55.00 61.420 8.00 4.00 1.420 44 gctaggcgatgctagctagct 416 21 0 57.14 60.424 10.00 8.00 1.424 45 gctagctaggcgatgctagct 412 21 0 57.14 60.424 9.00 8.00 1.424 46 gatcgatgtgcggctagcta 85 20 0 55.00 61.432 6.00 6.00 1.432 47 atcgatgtgcggctagcta 86 19 0 52.63 59.563 6.00 6.00 1.437 48 atcgtagcggcgatctagc 575 19 0 57.89 60.493 6.00 2.00 1.493 49 agcggcgatctagctagct 580 19 0 57.89 59.495 8.00 8.00 1.505 50 gctgactgatacgcgatgct 472 20 0 55.00 61.520 4.00 0.00 1.520 51 agctgactgatacgcgatgc 471 20 0 55.00 61.520 4.00 2.00 1.520 52 agctagctgactgatacgcg 467 20 0 55.00 58.460 8.00 4.00 1.540 53 gctgactgatacgcgatgc 472 19 0 57.89 60.553 4.00 2.00 1.553 54 tcgatgctagctaggcgatg 407 20 0 55.00 61.554 8.00 4.00 1.554 55 cggcgatctagctagctga 582 19 0 57.89 59.435 8.00 8.00 1.565 56 atcatcgatcgatgcatgc 440 19 0 47.37 60.587 12.00 6.00 1.587 57 agctgatcatcatcgatgct 515 20 0 45.00 58.361 8.00 8.00 1.639 58 cgtagcggcgatctagcta 577 19 0 57.89 59.339 6.00 6.00 1.661 59 gctagctgactgatcgatcga 109 21 0 52.38 60.661 8.00 8.00 1.661 60 gctactatcatctctgcgcga 356 21 0 52.38 60.662 4.00 4.00 1.662 61 atcgatgcatgcatgctacta 447 21 0 42.86 59.324 10.00 8.00 1.676 62 gatcgatgcatgcatgcta 446 19 0 47.37 59.309 10.00 8.00 1.691 63 tcggattagctagctgatgct 7 21 0 47.62 59.242 10.00 8.00 1.758 64 agcatcggattagctagctga 3 21 0 47.62 59.242 8.00 8.00 1.758 65 gcatcggattagctagctga 4 20 0 50.00 58.241 8.00 8.00 1.759 66 agctagctgatcatcgatgct 190 21 0 47.62 59.216 8.00 8.00 1.784 67 tgctagctagctgatcgatcg 558 21 0 52.38 60.787 10.00 6.00 1.787 68 cgatgctagctagctgatcga 555 21 0 52.38 60.787 10.00 7.00 1.787 69 tgactgatcgatcatcatgct 267 21 0 42.86 59.212 12.00 5.00 1.788 70 ctgactgatcgatcatcatgc 266 21 0 47.62 59.212 12.00 6.00 1.788 71 ctgatcgatcgatgctagct 399 20 0 50.00 58.173 10.00 4.00 1.827 72 agctgatcgatcgatgctag 397 20 0 50.00 58.173 10.00 8.00 1.827 73 ctagctgatcgatcgatgct 395 20 0 50.00 58.173 10.00 8.00 1.827 74 agctagctgatcgatcgatg 393 20 0 50.00 58.173 10.00 8.00 1.827 75 ctgatcgatcgatgctagct 118 20 0 50.00 58.173 10.00 4.00 1.827 76 agctagctgatcgatcgatg 73 20 0 50.00 58.173 10.00 8.00 1.827 77 gctagctgatcatcgatgct 191 20 0 50.00 58.173 8.00 8.00 1.827 78 agctagctgatcatcgatgc 190 20 0 50.00 58.173 8.00 6.00 1.827 79 gatcgatgctagctaggcgat 405 21 0 52.38 60.845 8.00 6.00 1.845 80 atcgatcgatgctagctaggc 402 21 0 52.38 60.845 10.00 6.00 1.845 81 gctagctgatcgatcgatgtg 74 21 0 52.38 60.920 10.00 8.00 1.920 82 gctgactgatcgatcgatgc 113 20 0 55.00 61.922 10.00 8.00 1.922 83 cgatgcatgcatgctactag 449 20 0 50.00 58.074 10.00 6.00 1.926 84 actgatcgatcgatgctagct 117 21 0 47.62 59.073 10.00 6.00 1.927 85 agctagctgatcgatcgatgt 73 21 0 47.62 59.073 10.00 6.00 1.927 86 ctgatgctagtgatgcatgct 20 21 0 47.62 59.068 6.00 4.00 1.932 87 agctgatgctagtgatgcatg 18 21 0 47.62 59.068 6.00 4.00 1.932 88 tgactgatcgatcatcatgc 267 20 0 45.00 58.063 12.00 6.00 1.937 89 gatcgatcgatgctagctagg 401 21 0 52.38 59.060 10.00 6.00 1.940 90 ctatcatctctgcgcgatcg 360 20 0 55.00 61.980 6.00 6.00 1.980 91 actatcatctctgcgcgatc 359 20 0 50.00 58.018 4.00 4.00 1.982 92 actgatcgatcgatgctagc 117 20 0 50.00 58.018 10.00 6.00 1.982 93 gctagctgatcgatcgatgt 74 20 0 50.00 58.018 10.00 6.00 1.982 94 agctagctagctgatcgatcg 389 21 0 52.38 59.013 12.00 6.00 1.987 95 agctagctagctgatcgatcg 69 21 0 52.38 59.013 12.00 6.00 1.987 96 ctgactgatcgatcgatgctag 114 22 0 50.00 60.000 10.00 8.00 2.000 97 ctagctgactgatcgatcgatg 110 22 0 50.00 60.000 10.00 8.00 2.000 98 gctagctagctgatcgatcg 559 20 0 55.00 57.998 10.00 6.00 2.002 99 gctagctagctgatcgatcg 390 20 0 55.00 57.998 10.00 6.00 2.002 100 gctagctagctgatcgatcg 70 20 0 55.00 57.998 10.00 6.00 2.002 101 tgactgatcgatcgatgctag 115 21 0 47.62 58.990 10.00 8.00 2.010 102 ctgactgatcgatcgatgcta 114 21 0 47.62 58.990 10.00 6.00 2.010 103 tagctgactgatcgatcgatg 111 21 0 47.62 58.990 10.00 8.00 2.010 104 aaagcatcggattagctagctg 1 22 0 45.45 60.030 8.00 6.00 2.030 105 tgatgctagtgatgcatgct 21 20 0 45.00 57.966 6.00 4.00 2.034 106 ctgatgctagtgatgcatgc 20 20 0 50.00 57.966 6.00 6.00 2.034 107 gctgatgctagtgatgcatg 19 20 0 50.00 57.966 6.00 4.00 2.034 108 tatcatctctgcgcgatcg 361 19 0 52.63 61.047 6.00 6.00 2.047 109 atgctagctaggcgatgcta 410 20 0 50.00 57.952 8.00 5.00 2.048 110 gatacgcgatgctagctagct 479 21 0 52.38 58.940 10.00 8.00 2.060 111 atgctagctaggcgatgctag 410 21 0 52.38 58.937 8.00 7.00 2.063 112 tcatcgatgctagctagctga 551 21 0 47.62 58.934 10.00 8.00 2.066 113 tcgatgcatgcatgctact 448 19 0 47.37 58.933 10.00 6.00 2.067 114 gctactgatcgatgctacatcg 308 22 0 50.00 59.895 8.00 8.00 2.105 115 ctgatcgatcgatgtgcg 79 18 0 55.56 59.889 10.00 4.00 2.111 116 gctagctagctgatcgatcgt 559 21 0 52.38 58.876 10.00 4.00 2.124 117 tgactgatcgatcgatgcta 115 20 0 45.00 57.875 10.00 6.00 2.125 118 tcgatgctacatcgatcga 316 19 0 47.37 58.849 10.00 8.00 2.151 119 cggcgatctagctagctgact 582 21 0 57.14 61.177 8.00 6.00 2.177 120 agcggcgatctagctagctg 580 20 0 60.00 62.179 8.00 6.00 2.179 121 tcgtagcggcgatctagct 576 19 0 57.89 61.184 4.00 4.00 2.184 122 gatcgatgcatgcatgctact 446 21 0 47.62 61.188 10.00 6.00 2.188 123 tagctactatcatctctgcgcg 354 22 0 50.00 59.806 6.00 4.00 2.194 124 actgatacgcgatgctagct 476 20 0 50.00 57.781 6.00 4.00 2.219 125 aagcatcggattagctagctg 2 21 0 47.62 58.779 8.00 6.00 2.221 126 tagctgactagctgatcgatcg 594 22 0 50.00 59.775 8.00 6.00 2.225 127 tagctagctgactgatcgatcg 107 22 0 50.00 59.775 8.00 6.00 2.225 128 tcgtagcggcgatctagc 576 18 0 61.11 60.228 4.00 2.00 2.228 129 ctgactgatcgatcatcatgct 266 22 0 45.45 60.249 12.00 5.00 2.249 130 gatgctagctaggcgatgc 409 19 0 57.89 58.740 8.00 4.00 2.260 131 tcgatgctagctaggcgat 407 19 0 52.63 58.738 8.00 6.00 2.262 132 atcgatgctagctaggcga 406 19 0 52.63 58.738 8.00 4.00 2.262 133 gatcgatgctagctaggcg 405 19 0 57.89 58.738 8.00 4.00 2.262 134 cgatcgatgctagctaggc 404 19 0 57.89 58.738 8.00 6.00 2.262 135 gatcgtagcggcgatctagc 574 20 0 60.00 62.271 8.00 2.00 2.271 136 atcgatgcatgcatgctactag 447 22 0 45.45 60.280 10.00 6.00 2.280 137 ctgactgatacgcgatgctag 473 21 0 52.38 58.715 4.00 4.00 2.285 138 ctagctgactgatacgcgatg 469 21 0 52.38 58.715 4.00 2.00 2.285 139 tcatcgatcgatgcatgc 441 18 0 50.00 60.297 12.00 6.00 2.297 140 gcggcgatctagctagctg 581 19 0 63.16 61.320 8.00 6.00 2.320 141 gactgatacgcgatgctagcta 475 22 0 50.00 59.680 6.00 6.00 2.320 142 tagctagctgactgatacgcga 466 22 0 50.00 60.326 8.00 3.00 2.326 143 atcgtagcggcgatctagcta 575 21 0 52.38 61.348 6.00 6.00 2.348 144 gctgactagctgatcgatcgta 596 22 0 50.00 59.643 8.00 6.00 2.357 145 tgactgatacgcgatgctag 474 20 0 50.00 57.632 4.00 4.00 2.368 146 ctgactgatacgcgatgcta 473 20 0 50.00 57.632 4.00 2.00 2.368 147 tagctgactgatacgcgatg 470 20 0 50.00 57.632 4.00 2.00 2.368 148 gatcgtagcggcgatctag 574 19 0 57.89 58.588 8.00 4.00 2.412 149 tagcggcgatctagctagc 579 19 0 57.89 58.547 8.00 6.00 2.453 150 agctgatcgatcgtagcgg 566 19 0 57.89 61.465 8.00 4.00 2.465 151 atcgatgtgcggctagct 86 18 0 55.56 59.533 6.00 4.00 2.467 152 atcggattagctagctgatgct 6 22 0 45.45 59.532 10.00 8.00 2.468 153 tagctagctgactgatacgcg 466 21 0 52.38 58.528 8.00 4.00 2.472 154 tgactgatacgcgatgctagc 474 21 0 52.38 61.473 6.00 6.00 2.473 155 gctgactgatacgcgatgcta 472 21 0 52.38 61.473 4.00 2.00 2.473 156 tagctgactgatacgcgatgc 470 21 0 52.38 61.473 4.00 2.00 2.473 157 gatcgatgtgcggctagct 85 19 0 57.89 61.479 6.00 4.00 2.479 158 ctgatcgatcgtagcggc 568 18 0 61.11 60.490 8.00 3.00 2.490 159 gctgatcgatcgtagcgg 567 18 0 61.11 60.490 8.00 5.00 2.490 160 aagcatcggattagctagctga 2 22 0 45.45 60.497 8.00 8.00 2.497 161 gatcgatgtgcggctagc 85 18 0 61.11 60.500 6.00 6.00 2.500 162 ctagctagctgactgatacgcg 465 22 0 54.55 59.491 8.00 4.00 2.509 163 atcgatcatcgatgctagct 546 20 0 45.00 57.480 11.00 8.00 2.520 164 ctgatcatcatcgatgctagct 517 22 0 45.45 59.462 8.00 6.00 2.538 165 agctgatcatcatcgatgctag 515 22 0 45.45 59.462 8.00 6.00 2.538 166 ctagctgatcatcatcgatgct 513 22 0 45.45 59.462 8.00 8.00 2.538 167 agctagctgatcatcatcgatg 511 22 0 45.45 59.462 8.00 8.00 2.538 168 ctgatcgatcatcatgctagct 270 22 0 45.45 59.462 10.00 5.00 2.538 169 tgatcatcatcgatgctagct 518 21 0 42.86 58.438 8.00 6.00 2.562 170 ctgatcatcatcgatgctagc 517 21 0 47.62 58.438 8.00 6.00 2.562 171 gctgatcatcatcgatgctag 516 21 0 47.62 58.438 8.00 6.00 2.562 172 agctgatcatcatcgatgcta 515 21 0 42.86 58.438 8.00 6.00 2.562 173 tagctgatcatcatcgatgct 514 21 0 42.86 58.438 8.00 8.00 2.562 174 ctagctgatcatcatcgatgc 513 21 0 47.62 58.438 8.00 6.00 2.562 175 gctagctgatcatcatcgatg 512 21 0 47.62 58.438 8.00 8.00 2.562 176 tgatcgatcatcatgctagct 271 21 0 42.86 58.438 10.00 5.00 2.562 177 ctgatcgatcatcatgctagc 270 21 0 47.62 58.438 10.00 6.00 2.562 178 agctgactagctgatcgatcgt 595 22 0 50.00 60.566 8.00 4.00 2.566 179 agctagctgactgatacgcgat 467 22 0 50.00 60.570 8.00 4.00 2.570 180 gatgtgcggctagctagct 89 19 0 57.89 58.394 10.00 8.00 2.606 181 cggattagctagctgatgct 8 20 0 50.00 57.392 10.00 8.00 2.608 182 agcatcggattagctagctg 3 20 0 50.00 57.392 8.00 6.00 2.608 183 catcgatcgatgctagtatgc 325 21 0 47.62 58.376 12.00 4.00 2.624 184 catcgatcgatgctagtatgct 325 22 0 45.45 59.370 12.00 4.00 2.630 185 atcgatcgatgcatgcatg 443 19 0 47.37 61.636 10.00 8.00 2.636 186 gatgctagctaggcgatgctag 409 22 0 54.55 60.642 8.00 7.00 2.642 187 actgatcgatcatcatgctagc 269 22 0 45.45 59.325 10.00 6.00 2.675 188 tgatcatcatcgatgctagc 518 20 0 45.00 57.307 8.00 6.00 2.693 189 gctgatcatcatcgatgcta 516 20 0 45.00 57.307 8.00 6.00 2.693 190 tagctgatcatcatcgatgc 514 20 0 45.00 57.307 8.00 6.00 2.693 191 tgatcgatcatcatgctagc 271 20 0 45.00 57.307 10.00 6.00 2.693 192 cgtagcggcgatctagct 577 18 0 61.11 59.301 4.00 4.00 2.699 193 tgcatgcatgctactagctagc 452 22 0 50.00 60.706 10.00 8.00 2.706 194 gctagctgatgctagtgatgca 15 22 0 50.00 60.707 7.00 4.00 2.707 195 gatcgatgcatgcatgct 446 18 0 50.00 59.265 10.00 8.00 2.735 196 atcgatgctagctaggcgatg 406 21 0 52.38 61.735 8.00 5.00 2.735 197 catcgatgctagctagctgatc 552 22 0 50.00 59.256 10.00 8.00 2.744 198 atcatcgatgctagctagctga 550 22 0 45.45 59.256 10.00 8.00 2.744 199 gatcatcgatgctagctagctg 549 22 0 50.00 59.256 10.00 6.00 2.744 200 agctagctgatcatcgatgcta 190 22 0 45.45 59.256 8.00 7.00 2.744 201 tagctagctgatcatcgatgct 189 22 0 45.45 59.256 8.00 8.00 2.744 202 ctagctagctgatcatcgatgc 188 22 0 50.00 59.256 8.00 6.00 2.744 203 gctagctagctgatcatcgatg 187 22 0 50.00 59.256 10.00 8.00 2.744 204 tgactgatcgatcatcatgcta 267 22 0 40.91 59.256 12.00 5.00 2.744 205 gctagctgatcatcgatgcta 191 21 0 47.62 58.255 8.00 7.00 2.745 206 tagctagctgatcatcgatgc 189 21 0 47.62 58.255 8.00 6.00 2.745 207 tcgatcatcgatgctagctag 547 21 0 47.62 58.254 9.00 8.00 2.746 208 tgatcgatcgatgctagctag 400 21 0 47.62 58.254 10.00 8.00 2.746 209 ctgatcgatcgatgctagcta 399 21 0 47.62 58.254 10.00 6.00 2.746 210 tagctgatcgatcgatgctag 396 21 0 47.62 58.254 10.00 8.00 2.746 211 tagctagctgatcgatcgatg 392 21 0 47.62 58.254 10.00 8.00 2.746 212 tgatcgatcgatgctagctag 119 21 0 47.62 58.254 10.00 8.00 2.746 213 ctgatcgatcgatgctagcta 118 21 0 47.62 58.254 10.00 6.00 2.746 214 tagctagctgatcgatcgatg 72 21 0 47.62 58.254 10.00 8.00 2.746 215 ctgatcgatcgatgctagctag 399 22 0 50.00 59.252 10.00 8.00 2.748 216 ctagctagctgatcgatcgatg 391 22 0 50.00 59.252 10.00 8.00 2.748 217 ctgatcgatcgatgctagctag 118 22 0 50.00 59.252 10.00 8.00 2.748 218 ctagctagctgatcgatcgatg 71 22 0 50.00 59.252 10.00 8.00 2.748 219 acatcgatcgatgctagtatgc 324 22 0 45.45 59.236 12.00 4.00 2.764 220 tcgatgtgcggctagcta 87 18 0 55.56 59.232 6.00 6.00 2.768 221 agctagctagctgatcgatcga 389 22 0 50.00 60.769 12.00 8.00 2.769 222 agctagctagctgatcgatcga 69 22 0 50.00 60.769 12.00 8.00 2.769 223 gatgcatgcatgctactagct 450 21 0 47.62 58.187 10.00 5.00 2.813 224 atcgatgcatgcatgctac 447 19 0 47.37 58.187 10.00 8.00 2.813 225 atcgatcgatgctagctagg 402 20 0 50.00 57.180 10.00 6.00 2.820 226 tcgatcatcgatgctagcta 547 20 0 45.00 57.158 9.00 6.00 2.842 227 tgatcgatcgatgctagcta 400 20 0 45.00 57.158 10.00 6.00 2.842 228 tgatcgatcgatgctagcta 119 20 0 45.00 57.158 10.00 6.00 2.842 229 cgatgcatgcatgctactagc 449 21 0 52.38 61.851 10.00 7.00 2.851 230 atacgcgatgctagctagct 480 20 0 50.00 57.129 10.00 8.00 2.871 231 gctgatcatcgatgctactagc 195 22 0 50.00 59.121 8.00 8.00 2.879 232 gctagctgatcatcgatgctac 191 22 0 50.00 59.121 8.00 6.00 2.879 233 ctactatcatctctgcgcgatc 357 22 0 50.00 59.118 4.00 4.00 2.882 234 tgatcgatcgatgctagctagt 119 22 0 45.45 59.118 10.00 6.00 2.882 235 actgatcgatcgatgctagcta 117 22 0 45.45 59.118 10.00 6.00 2.882 236 tagctagctgatcgatcgatgt 72 22 0 45.45 59.118 10.00 6.00 2.882 237 tgatgctagtgatgcatgctag 21 22 0 45.45 59.115 8.00 8.00 2.885 238 ctgatgctagtgatgcatgcta 20 22 0 45.45 59.115 6.00 6.00 2.885 239 tagctgatgctagtgatgcatg 17 22 0 45.45 59.115 6.00 4.00 2.885 240 gctactatcatctctgcgcgat 356 22 0 50.00 60.887 4.00 4.00 2.887 241 tactatcatctctgcgcgatc 358 21 0 47.62 58.106 4.00 4.00 2.894 242 gactgatcgatcgatgctagct 116 22 0 50.00 60.895 10.00 6.00 2.895 243 gctgatgctagtgatgcatgc 19 21 0 52.38 61.911 6.00 6.00 2.911 244 gatgcatgcatgctactagc 450 20 0 50.00 57.086 10.00 7.00 2.914 245 gctgatcgatcgatgctagct 398 21 0 52.38 61.918 10.00 6.00 2.918 246 gctagctgatcgatcgatgct 394 21 0 52.38 61.918 10.00 8.00 2.918 247 agctagctgatcgatcgatgc 393 21 0 52.38 61.918 10.00 8.00 2.918 248 tgatgctagtgatgcatgcta 21 21 0 42.86 58.060 6.00 6.00 2.940 249 tatcatctctgcgcgatcga 361 20 0 50.00 62.940 6.00 4.00 2.940 250 tagctagctagctgatcgatcg 388 22 0 50.00 59.059 12.00 6.00 2.941 251 tagctagctagctgatcgatcg 68 22 0 50.00 59.059 12.00 6.00 2.941 252 cgatgtgcggctagctagct 88 20 0 60.00 62.979 10.00 8.00 2.979 253 ctgatacgcgatgctagctag 477 21 0 52.38 58.020 8.00 8.00 2.980 254 gcatgcatgctactagctagc 453 21 0 52.38 58.018 10.00 8.00 2.982 255 tcgtagcggcgatctagctag 576 21 0 57.14 61.990 8.00 8.00 2.990 256 gcatgcatgctactagctagct 453 22 0 50.00 58.997 10.00 8.00 3.003 257 atgctagctagctgatcgatcg 557 22 0 50.00 61.005 10.00 6.00 3.005 258 cgatgctagctagctgatcgat 555 22 0 50.00 61.005 10.00 8.00 3.005 259 cgatcatcgatgctagctagct 548 22 0 50.00 61.005 10.00 8.00 3.005 260 ctgatcgatcgatgctagctagt 118 23 0 47.83 60.041 10.00 6.00 3.041 261 actgatcgatcgatgctagctag 117 23 0 47.83 60.041 10.00 8.00 3.041 262 ctagctagctgatcgatcgatgt 71 23 0 47.83 60.041 10.00 6.00 3.041 263 gctgatcatcgatgctactagct 195 23 0 47.83 60.048 8.00 5.00 3.048 264 agctgatcatcgatgctactagc 194 23 0 47.83 60.048 8.00 8.00 3.048 265 gctagctgatcatcgatgctact 191 23 0 47.83 60.048 8.00 6.00 3.048 266 agctagctgatcatcgatgctac 190 23 0 47.83 60.048 8.00 6.00 3.048 267 ctagctagctagctgatcgatcg 387 23 0 52.17 59.952 12.00 6.00 3.048 268 ctagctagctagctgatcgatcg 67 23 0 52.17 59.952 12.00 6.00 3.048 269 tgatgctagtgatgcatgctagt 21 23 0 43.48 59.947 8.00 6.00 3.053 270 cgatcgatgctagtatgctagct 328 23 0 47.83 60.063 10.00 8.00 3.063 271 agctagctgatgctagtgatgc 14 22 0 50.00 58.936 8.00 2.00 3.064 272 gctagctagctgatcgatcgta 559 22 0 50.00 58.927 10.00 6.00 3.073 273 tcgatcgatgctagctagtagc 122 22 0 50.00 58.927 8.00 8.00 3.073 274 ctgatgctagtgatgcatgctag 20 23 0 47.83 60.074 8.00 8.00 3.074 275 ctagctgatgctagtgatgcatg 16 23 0 47.83 60.074 7.00 4.00 3.074 276 atctctgcgcgatcgatg 365 18 0 55.56 61.080 6.00 4.00 3.080 277 catctctgcgcgatcgat 364 18 0 55.56 61.080 6.00 6.00 3.080 278 atcatctctgcgcgatcg 362 18 0 55.56 61.080 6.00 6.00 3.080 279 gctagctgatgctagtgatgc 15 21 0 52.38 57.917 7.00 2.00 3.083 280 tagcggcgatctagctagctg 579 21 0 57.14 62.111 8.00 6.00 3.111 281 actgatacgcgatgctagcta 476 21 0 47.62 57.875 6.00 6.00 3.125 282 atgctagctaggcgatgct 410 19 0 52.63 57.861 8.00 3.00 3.139 283 actgatacgcgatgctagctag 476 22 0 50.00 58.860 8.00 8.00 3.140 284 catgcatgctactagctagctga 454 23 0 47.83 59.856 8.00 6.00 3.144 285 cggcgatctagctagctgacta 582 22 0 54.55 61.145 8.00 6.00 3.145 286 gatcgatgcatgcatgctacta 446 22 0 45.45 61.154 10.00 8.00 3.154 287 acatcgatcgatgctagtatgct 324 23 0 43.48 60.157 12.00 4.00 3.157 288 tcggattagctagctgatgctag 7 23 0 47.83 60.160 10.00 7.00 3.160 289 gctagctactatcatctctgcgc 352 23 0 52.17 59.838 6.00 4.00 3.162 290 gctagctagctactgatcgatgc 300 23 0 52.17 59.838 10.00 4.00 3.162 291 gctagctagctgatcgatcgtag 559 23 0 52.17 59.832 10.00 8.00 3.168 292 ctagctagctgatcatcgatgct 188 23 0 47.83 60.170 8.00 8.00 3.170 293 agctagctagctgatcatcgatg 186 23 0 47.83 60.170 12.00 8.00 3.170 294 gctagctgactagctgatcgatc 592 23 0 52.17 59.808 8.00 8.00 3.192 295 gctagctagctgactgatcgatc 105 23 0 52.17 59.808 10.00 8.00 3.192 296 cgcgatcgatgctagcta 372 18 0 55.56 58.807 6.00 6.00 3.193 297 gctatcgatcatcgatgctagc 543 22 0 50.00 61.206 11.00 8.00 3.206 298 gcatcggattagctagctgatg 4 22 0 50.00 61.220 10.00 8.00 3.220 299 tgactgatcgatcgatgct 115 19 0 47.37 57.768 10.00 6.00 3.232 300 ctgactgatcgatcgatgc 114 19 0 52.63 57.768 10.00 8.00 3.232 301 gctgactgatcgatcgatg 113 19 0 52.63 57.768 10.00 8.00 3.232 302 agctaggcgatgctagctagct 415 22 0 54.55 61.245 10.00 8.00 3.245 303 aggcgatgctagctagctact 419 21 0 52.38 57.752 10.00 6.00 3.248 304 tgactgatcgatcatcatgctag 267 23 0 43.48 60.250 12.00 5.00 3.250 305 ctgactgatcgatcatcatgcta 266 23 0 43.48 60.250 12.00 4.00 3.250 306 actagctagctgactgatacgcg 464 23 0 52.17 60.254 8.00 4.00 3.254 307 actgatcgatcatcatgctagct 269 23 0 43.48 60.271 10.00 5.00 3.271 308 ctagctgatcgatcgtagcgg 564 21 0 57.14 62.273 8.00 4.00 3.273 309 tagctgatcgatcgtagcg 565 19 0 52.63 57.716 8.00 4.00 3.284 310 gatcgatgtgcggctagctag 85 21 0 57.14 62.288 8.00 8.00 3.288 311 agctagctgactagctgatcga 591 22 0 50.00 58.678 9.00 4.00 3.322 312 agctagctagctgactgatcga 104 22 0 50.00 58.678 12.00 4.00 3.322 313 cgatcgtagcggcgatcta 573 19 0 57.89 62.347 8.00 4.00 3.347 314 catcggattagctagctgatg 5 21 0 47.62 57.647 10.00 8.00 3.353 315 gctagctgactagctgatcga 592 21 0 52.38 57.646 7.00 4.00 3.354 316 gctagctagctgactgatcga 105 21 0 52.38 57.646 10.00 4.00 3.354 317 atcgatcatcgatgctagctag 546 22 0 45.45 58.608 11.00 8.00 3.392 318 ctatcgatcatcgatgctagct 544 22 0 45.45 58.608 11.00 8.00 3.392 319 agctgactgatacgcgatgct 471 21 0 52.38 62.397 4.00 0.00 3.397 320 gctagctgatcgatcgtagcg 563 21 0 57.14 62.412 10.00 6.00 3.412 321 tcgatcgatgcatgcatg 444 18 0 50.00 61.413 10.00 8.00 3.413 322 catcgatcgatgcatgca 442 18 0 50.00 61.413 12.00 8.00 3.413 323 atcgatcatcgatgctagcta 546 21 0 42.86 57.587 11.00 6.00 3.413 324 tatcgatcatcgatgctagct 545 21 0 42.86 57.587 11.00 8.00 3.413 325 ctatcgatcatcgatgctagc 544 21 0 47.62 57.587 11.00 6.00 3.413 326 gctatcgatcatcgatgctag 543 21 0 47.62 57.587 11.00 8.00 3.413 327 tgctagctaggcgatgcta 411 19 0 52.63 57.552 8.00 5.00 3.448 328 ctgactgatacgcgatgct 473 19 0 52.63 57.520 4.00 0.00 3.480 329 agctgactgatacgcgatg 471 19 0 52.63 57.520 4.00 2.00 3.480 330 tgatcatcatcgatgctagcta 518 22 0 40.91 58.509 8.00 6.00 3.491 331 tagctgatcatcatcgatgcta 514 22 0 40.91 58.509 8.00 8.00 3.491 332 tgatcgatcatcatgctagcta 271 22 0 40.91 58.509 10.00 6.00 3.491 333 cggattagctagctgatgcta 8 21 0 47.62 57.498 10.00 6.00 3.502 334 tgatacgcgatgctagctagct 478 22 0 50.00 61.508 10.00 8.00 3.508 335 tgatcatcatcgatgctagctag 518 23 0 43.48 59.492 8.00 8.00 3.508 336 ctgatcatcatcgatgctagcta 517 23 0 43.48 59.492 8.00 6.00 3.508 337 tagctgatcatcatcgatgctag 514 23 0 43.48 59.492 8.00 6.00 3.508 338 ctagctgatcatcatcgatgcta 513 23 0 43.48 59.492 8.00 8.00 3.508 339 tagctagctgatcatcatcgatg 510 23 0 43.48 59.492 8.00 8.00 3.508 340 ctgatcgatcatcatgctagcta 270 23 0 43.48 59.492 10.00 6.00 3.508 341 cggattagctagctgatgctag 8 22 0 50.00 58.481 10.00 7.00 3.519 342 gcggcgatctagctagct 581 18 0 61.11 58.478 8.00 8.00 3.522 343 agcggcgatctagctagc 580 18 0 61.11 58.478 8.00 6.00 3.522 344 cgatcgatgtgcggctag 84 18 0 61.11 61.523 6.00 4.00 3.523 345 aaagcatcggattagctagct 1 21 0 42.86 57.475 8.00 8.00 3.525 346 tgctagctagctgatcgatcgt 558 22 0 50.00 61.537 10.00 4.00 3.537 347 agctactatcatctctgcgcga 355 22 0 50.00 61.537 4.00 4.00 3.537 348 gctgactagctgatcgatcgtag 596 23 0 52.17 60.556 8.00 8.00 3.556 349 agctgactagctgatcgatcgta 595 23 0 47.83 60.556 8.00 6.00 3.556 350 tagctgactagctgatcgatcgt 594 23 0 47.83 60.556 8.00 4.00 3.556 351 gactgatacgcgatgctagctag 475 23 0 52.17 60.560 8.00 8.00 3.560 352 tagctagctgactgatacgcgat 466 23 0 47.83 60.560 8.00 4.00 3.560 353 agctagctgactgatcgatcga 108 22 0 50.00 61.569 8.00 8.00 3.569 354 atcatcgatgctagctagctg 550 21 0 47.62 57.405 10.00 6.00 3.595 355 catcgatcgatgctagtatgcta 325 23 0 43.48 59.402 12.00 6.00 3.598 356 gcgcgatcgatgctagcta 371 19 0 57.89 62.627 6.00 6.00 3.627 357 tcgatgctagctaggcga 407 18 0 55.56 58.362 8.00 4.00 3.638 358 tgatcgatcatcatgctagctac 271 23 0 43.48 59.360 10.00 4.00 3.640 359 gctagctgactgatacgcg 468 19 0 57.89 57.357 6.00 4.00 3.643 360 gatgctagctagctgatcgatc 556 22 0 50.00 58.355 10.00 8.00 3.645 361 ctagctactatcatctctgcgcg 353 23 0 52.17 60.675 6.00 4.00 3.675 362 ctagctgactagctgatcgatcg 593 23 0 52.17 60.675 8.00 6.00 3.675 363 ctagctagctgactgatcgatcg 106 23 0 52.17 60.675 8.00 6.00 3.675 364 atcgatcgatgtgcggcta 82 19 0 52.63 62.688 10.00 2.00 3.688 365 atcgatcgtagcggcgat 571 18 0 55.56 61.698 6.00 6.00 3.698 366 cgatgtgcggctagctag 88 18 0 61.11 58.288 8.00 8.00 3.712 367 actatcatctctgcgcgatcg 359 21 0 52.38 62.718 6.00 6.00 3.718 368 tgatcgatcgatgctagctagg 400 22 0 50.00 61.720 10.00 6.00 3.720 369 tacatcgatcgatgctagtatgc 323 23 0 43.48 59.274 12.00 4.00 3.726 370 gatgcatgcatgctactagcta 450 22 0 45.45 58.265 10.00 6.00 3.735 371 cggattagctagctgatgctagt 8 23 0 47.83 59.261 10.00 6.00 3.739 372 ctactatcatctctgcgcgat 357 21 0 47.62 57.255 4.00 4.00 3.745 373 tagctagctagctgatcgatcga 388 23 0 47.83 60.752 12.00 8.00 3.752 374 tagctagctagctgatcgatcga 68 23 0 47.83 60.752 12.00 8.00 3.752 375 gatgcatgcatgctactagctag 450 23 0 47.83 59.227 10.00 8.00 3.773 376 atcgatcgatgctagctagtagc 121 23 0 47.83 59.226 10.00 8.00 3.774 377 agctactgatcgatgctacatcg 307 23 0 47.83 60.793 8.00 8.00 3.793 378 agctagctgactgatcgatcat 260 22 0 45.45 58.201 10.00 8.00 3.799 379 agctagctgatcgatcgatgtg 73 22 0 50.00 61.811 10.00 8.00 3.811 380 ctgcgcgatcgatgctag 369 18 0 61.11 61.813 6.00 4.00 3.813 381 gctgactgatcgatcgatgct 113 21 0 52.38 62.815 10.00 6.00 3.815 382 agctgactgatcgatcgatgc 112 21 0 52.38 62.815 10.00 8.00 3.815 383 gctgatcatcatcgatgct 516 19 0 47.37 57.176 8.00 8.00 3.824 384 agctgatcatcatcgatgc 515 19 0 47.37 57.176 8.00 6.00 3.824 385 atcgatgctacatcgatcg 315 19 0 47.37 57.160 12.00 6.00 3.840 386 tgatcgatcgatgctagctagta 119 23 0 43.48 59.159 10.00 8.00 3.841 387 gctgatcgatcgatgctagcta 398 22 0 50.00 61.859 10.00 6.00 3.859 388 tagctagctgatcgatcgatgc 392 22 0 50.00 61.859 10.00 8.00 3.859 389 agctagctagctgatcatcga 186 21 0 47.62 57.134 12.00 6.00 3.866 390 gactgatcgatcgatgctagcta 116 23 0 47.83 60.873 10.00 6.00 3.873 391 gctagctgactgatcgatcat 261 21 0 47.62 57.117 10.00 8.00 3.883 392 cgatcgatgctagctagtagc 123 21 0 52.38 57.116 8.00 8.00 3.884 393 catgcatgctactagctagctg 454 22 0 50.00 58.085 8.00 8.00 3.915 394 atgcatgcatgctactagctagc 451 23 0 47.83 60.921 10.00 8.00 3.921 395 gctagctgatgctagtgatgcat 15 23 0 47.83 60.929 7.00 6.00 3.929 396 gctagctgactagctgatcgat 592 22 0 50.00 58.034 7.00 6.00 3.966 397 gctagctagctgactgatcgat 105 22 0 50.00 58.034 10.00 6.00 3.966 398 tgcatgcatgctactagctag 452 21 0 47.62 57.032 10.00 8.00 3.968 399 tcgatcatcgatgctagct 547 19 0 47.37 57.026 9.00 4.00 3.974 400 tgatcgatcgatgctagct 400 19 0 47.37 57.026 10.00 4.00 3.974 401 ctgatcgatcgatgctagc 399 19 0 52.63 57.026 10.00 6.00 3.974 402 gctgatcgatcgatgctag 398 19 0 52.63 57.026 10.00 8.00 3.974 403 agctgatcgatcgatgcta 397 19 0 47.37 57.026 10.00 6.00 3.974 404 tagctgatcgatcgatgct 396 19 0 47.37 57.026 10.00 8.00 3.974 405 ctagctgatcgatcgatgc 395 19 0 52.63 57.026 10.00 8.00 3.974 406 gctagctgatcgatcgatg 394 19 0 52.63 57.026 10.00 8.00 3.974 407 ctatcatctctgcgcgatc 360 19 0 52.63 57.026 4.00 4.00 3.974 408 tgatcgatcgatgctagct 119 19 0 47.37 57.026 10.00 4.00 3.974 409 ctgatcgatcgatgctagc 118 19 0 52.63 57.026 10.00 6.00 3.974 410 gctagctgatcgatcgatg 74 19 0 52.63 57.026 10.00 8.00 3.974 411 gctagctgatcatcgatgc 191 19 0 52.63 57.021 8.00 6.00 3.979 412 tcatcgatgctagctagctgatc 551 23 0 47.83 61.000 10.00 8.00 4.000 413 gatcatcgatgctagctagctga 549 23 0 47.83 61.000 10.00 8.00 4.000 414 agctagctgactagctgatcgat 591 23 0 47.83 58.998 9.00 6.00 4.002 415 agctagctagctgactgatcgat 104 23 0 47.83 58.998 12.00 6.00 4.002 416 tagctagctgatgctagtgatgc 13 23 0 47.83 58.984 8.00 2.00 4.016 417 cgatgctactagctagctgatca 204 23 0 47.83 58.981 8.00 6.00 4.019 418 catcgatgctactagctagctga 201 23 0 47.83 58.981 8.00 6.00 4.019 419 tcatcgatgctactagctagctg 200 23 0 47.83 58.981 8.00 7.00 4.019 420 tgatgctagtgatgcatgctagta 21 24 0 41.67 59.959 8.00 8.00 4.041 421 tgatcgatcgatgctagctagtag 119 24 0 45.83 60.049 10.00 6.00 4.049 422 ctgatcgatcgatgctagctagta 118 24 0 45.83 60.049 10.00 8.00 4.049 423 tcatcatcgatgctagctagtagc 521 24 0 45.83 60.056 8.00 8.00 4.056 424 tcgatcatcatgctagctactagc 274 24 0 45.83 60.056 8.00 8.00 4.056 425 tgatcatcgatgctactagctagc 197 24 0 45.83 60.056 8.00 8.00 4.056 426 gctgatcatcgatgctactagcta 195 24 0 45.83 60.056 8.00 6.00 4.056 427 tagctgatcatcgatgctactagc 193 24 0 45.83 60.056 8.00 8.00 4.056 428 gctagctgatcatcgatgctacta 191 24 0 45.83 60.056 8.00 8.00 4.056 429 tagctagctgatcatcgatgctac 189 24 0 45.83 60.056 10.00 6.00 4.056 430 cgatcgatgctagtatgctagcta 328 24 0 45.83 60.070 10.00 8.00 4.070 431 gctaggcgatgctagctagctac 416 23 0 56.52 61.109 10.00 8.00 4.109 432 ggcgatctagctagctgactagc 583 23 0 56.52 61.123 8.00 8.00 4.123 433 gcgatctagctagctgactagc 584 22 0 54.55 57.876 8.00 8.00 4.124 434 gactgatcgatcatcatgctagc 268 23 0 47.83 61.135 10.00 6.00 4.135 435 atcgtagcggcgatctagctag 575 22 0 54.55 62.138 8.00 8.00 4.138 436 gctagctactgatcgatgctaca 304 23 0 47.83 58.849 6.00 3.00 4.151 437 gctgatcatcatcgatgctagc 516 22 0 50.00 62.158 8.00 8.00 4.158 438 gctagctgatcatcatcgatgc 512 22 0 50.00 62.158 8.00 7.00 4.158 439 acatcgatcgatgctagtatgcta 324 24 0 41.67 60.161 12.00 6.00 4.161 440 tacatcgatcgatgctagtatgct 323 24 0 41.67 60.161 12.00 4.00 4.161 441 aggcgatgctagctagctacta 419 22 0 50.00 57.839 10.00 8.00 4.161 442 taggcgatgctagctagctact 418 22 0 50.00 57.839 10.00 6.00 4.161 443 ctaggcgatgctagctagctac 417 22 0 54.55 57.839 10.00 8.00 4.161 444 tagctagctagctgatcatcgatg 185 24 0 45.83 60.174 12.00 8.00 4.174 445 ctagctagctgactgatacgcga 465 23 0 52.17 61.184 8.00 3.00 4.184 446 ttagctagctgatgctagtgatgc 12 24 0 45.83 60.212 8.00 2.00 4.212 447 tagctaggcgatgctagctagct 414 23 0 52.17 61.214 10.00 8.00 4.214 448 agctagctactgatcgatgctaca 303 24 0 45.83 59.754 8.00 2.00 4.246 449 ctaggcgatgctagctagctact 417 23 0 52.17 58.754 10.00 6.00 4.246 450 tactagctagctgactgatacgcg 463 24 0 50.00 60.255 8.00 4.00 4.255 451 tcgatgcatgcatgctac 448 18 0 50.00 57.745 10.00 8.00 4.255 452 ggcgatctagctagctgactag 583 22 0 54.55 57.739 8.00 8.00 4.261 453 tagctagctgactagctgatcga 590 23 0 47.83 58.736 9.00 4.00 4.264 454 tagctagctagctgactgatcga 103 23 0 47.83 58.736 12.00 4.00 4.264 455 actatcatcgatcgatgcatgc 437 22 0 45.45 62.270 12.00 8.00 4.270 456 tgatcatcatcgatgctagctagt 518 24 0 41.67 60.271 8.00 6.00 4.271 457 tgatcgatcatcatgctagctact 271 24 0 41.67 60.271 10.00 2.00 4.271 458 ctgatcgatcatcatgctagctac 270 24 0 45.83 60.271 10.00 4.00 4.271 459 actgatcgatcatcatgctagcta 269 24 0 41.67 60.271 10.00 6.00 4.271 460 catcgatcgatgctagtatgctag 325 24 0 45.83 60.278 12.00 8.00 4.278 461 tgcatgctactagctagctgac 456 22 0 50.00 57.681 8.00 6.00 4.319 462 tgactgatacgcgatgctagct 474 22 0 50.00 62.319 6.00 4.00 4.319 463 ctgactgatacgcgatgctagc 473 22 0 54.55 62.319 6.00 6.00 4.319 464 gctgactgatacgcgatgctag 472 22 0 54.55 62.319 4.00 4.00 4.319 465 agctgactgatacgcgatgcta 471 22 0 50.00 62.319 4.00 2.00 4.319 466 tagctgactgatacgcgatgct 470 22 0 50.00 62.319 4.00 0.00 4.319 467 ctagctgactgatacgcgatgc 469 22 0 54.55 62.319 4.00 2.00 4.319 468 gctagctgactgatacgcgatg 468 22 0 54.55 62.319 6.00 2.00 4.319 469 gcatgctactagctagctgactg 457 23 0 52.17 58.672 8.00 6.00 4.328 470 tgcatgctactagctagctgact 456 23 0 47.83 58.672 8.00 6.00 4.328 471 tatcgatcatcgatgctagctag 545 23 0 43.48 58.667 11.00 8.00 4.333 472 tgctagtgatgcatgctagtagtg 24 24 0 45.83 59.645 8.00 5.00 4.355 473 ctagctagctgactagctgatcga 589 24 0 50.00 59.644 9.00 4.00 4.356 474 tctagctagctgactagctgatcg 588 24 0 50.00 59.644 9.00 4.00 4.356 475 ctagctagctagctgactgatcga 102 24 0 50.00 59.644 12.00 4.00 4.356 476 gcatgctactagctagctgactga 457 24 0 50.00 60.373 8.00 4.00 4.373 477 atcggattagctagctgatgctag 6 24 0 45.83 60.387 10.00 8.00 4.387 478 ctgatcatcatcgatgctagctag 517 24 0 45.83 60.391 8.00 8.00 4.391 479 ctagctagctgatcatcatcgatg 509 24 0 45.83 60.391 8.00 8.00 4.391 480 agctgatcgatcgtagcg 566 18 0 55.56 57.606 8.00 4.00 4.394 481 ttagctagctgactgatcgatcat 258 24 0 41.67 59.570 10.00 8.00 4.430 482 cgatcgtagcggcgatct 573 18 0 61.11 62.431 8.00 6.00 4.431 483 agctagctagctgatcatcgat 186 22 0 45.45 57.542 12.00 6.00 4.458 484 tgctagctagctgatcgatcgta 558 23 0 47.83 61.493 10.00 6.00 4.493 485 tagctactatcatctctgcgcga 354 23 0 47.83 61.493 6.00 4.00 4.493 486 gatcgatcgatgctagctaggc 401 22 0 54.55 62.516 10.00 6.00 4.516 487 tagctagctgactgatcgatcga 107 23 0 47.83 61.523 8.00 8.00 4.523 488 gatcgtagcggcgatcta 574 18 0 55.56 57.475 8.00 2.00 4.525 489 tcgatcgatgtgcggcta 83 18 0 55.56 62.543 8.00 2.00 4.543 490 gcatgcatgctactagctagctg 453 23 0 52.17 61.544 10.00 8.00 4.544 491 tgcatgcatgctactagctagct 452 23 0 47.83 61.544 10.00 8.00 4.544 492 tagctgactagctgatcgatcgta 594 24 0 45.83 60.546 8.00 6.00 4.546 493 atgcatgcatgctactagctag 451 22 0 45.45 57.449 10.00 8.00 4.551 494 cggcgatctagctagctg 582 18 0 61.11 57.442 8.00 6.00 4.558 495 tgctagctaggcgatgct 411 18 0 55.56 57.439 8.00 3.00 4.561 496 agctagctgatgctagtgatgca 14 23 0 47.83 61.574 8.00 4.00 4.574 497 catgcatgctactagctagctgac 454 24 0 50.00 60.603 8.00 6.00 4.603 498 ctagctagctgatcatcatcga 509 22 0 45.45 57.392 8.00 7.00 4.608 499 gactgatcgatcatcatgctag 268 22 0 45.45 57.387 10.00 5.00 4.613 500 tactatcatctctgcgcgatcg 358 22 0 50.00 62.629 6.00 6.00 4.629 501 tcgatcgatgctagtatgctag 327 22 0 45.45 57.367 8.00 8.00 4.633 502 aaagcatcggattagctagctga 1 23 0 43.48 61.634 8.00 8.00 4.634 503 catcatcgatgctagctagtagc 522 23 0 47.83 58.360 8.00 8.00 4.640 504 cgatcatcatgctagctactagc 275 23 0 47.83 58.360 8.00 8.00 4.640 505 tcgatgctactagctagctgatca 203 24 0 45.83 60.670 8.00 6.00 4.670 506 ctagctgatgctagtgatgcat 16 22 0 45.45 57.329 7.00 6.00 4.671 507 agctagctgactagctgatcgatc 591 24 0 50.00 60.672 9.00 8.00 4.672 508 agctagctagctgactgatcgatc 104 24 0 50.00 60.672 12.00 8.00 4.672 509 agctagctactatcatctctgcgc 351 24 0 50.00 60.672 8.00 4.00 4.672 510 gctagctagctactgatcgatgct 300 24 0 50.00 60.672 10.00 4.00 4.672 511 agctagctagctactgatcgatgc 299 24 0 50.00 60.672 12.00 4.00 4.672 512 cgatgcatgcatgctactagct 449 22 0 50.00 62.673 10.00 5.00 4.673 513 atgctagctagctgatcgatcgt 557 23 0 47.83 61.711 10.00 4.00 4.711 514 agctactatcatctctgcgcgat 355 23 0 47.83 61.711 4.00 4.00 4.711 515 tgactgatcgatcgatgctagc 115 22 0 50.00 62.719 10.00 6.00 4.719 516 gctgactgatcgatcgatgcta 113 22 0 50.00 62.719 10.00 6.00 4.719 517 tagctgactgatcgatcgatgc 111 22 0 50.00 62.719 10.00 8.00 4.719 518 gcgcgatcgatgctagct 371 18 0 61.11 62.722 6.00 4.00 4.722 519 actagctagctgatcatcatcga 508 23 0 43.48 58.277 8.00 7.00 4.723 520 tagctagctgactgatcgatcat 259 23 0 43.48 58.277 10.00 8.00 4.723 521 cgatgctactagctagctgatcat 204 24 0 45.83 59.274 8.00 8.00 4.726 522 catcgatgctactagctagctgat 201 24 0 45.83 59.274 8.00 8.00 4.726 523 atcatcgatgctactagctagctg 199 24 0 45.83 59.274 8.00 7.00 4.726 524 agctagctgatcgatcgatgct 393 22 0 50.00 62.732 10.00 8.00 4.732 525 ggattagctagctgatgctagtga 9 24 0 45.83 59.252 10.00 5.00 4.748 526 tagctagctagctgatcatcga 185 22 0 45.45 57.250 12.00 6.00 4.750 527 ctagctagctagctgatcatcga 184 23 0 47.83 58.249 12.00 6.00 4.751 528 tagctagctgatcgatcgatgtg 72 23 0 47.83 61.756 10.00 8.00 4.756 529 gctgatgctagtgatgcatgct 19 22 0 50.00 62.764 6.00 4.00 4.764 530 agctgatgctagtgatgcatgc 18 22 0 50.00 62.764 6.00 6.00 4.764 531 gtagcggcgatctagctagctg 578 22 0 59.09 62.774 8.00 6.00 4.774 532 tagctactgatcgatgctacatcg 306 24 0 45.83 60.775 8.00 8.00 4.775 533 actgatcgatcgatgctagctagt 117 24 0 45.83 60.775 10.00 6.00 4.775 534 atcgatcgatgtgcggct 82 18 0 55.56 62.790 10.00 1.00 4.790 535 catcgatgctactagctagctg 201 22 0 50.00 57.193 8.00 7.00 4.807 536 atgcatgctactagctagctga 455 22 0 45.45 57.189 8.00 6.00 4.811 537 gatgctagtgatgcatgctagt 22 22 0 45.45 57.176 8.00 6.00 4.824 538 tctgcgcgatcgatgcta 368 18 0 55.56 62.829 6.00 2.00 4.829 539 ctgatgctagtgatgcatgctagt 20 24 0 45.83 60.835 8.00 6.00 4.835 540 gatcgatcgatgctagctagtagc 120 24 0 50.00 60.846 10.00 8.00 4.846 541 gctagctactgatcgatgctacat 304 24 0 45.83 59.150 6.00 4.00 4.850 542 atcgatgcatgcatgcta 447 18 0 44.44 57.148 10.00 8.00 4.852 543 tcggattagctagctgatgctagt 7 24 0 45.83 60.863 10.00 6.00 4.863 544 tcgatgctactagctagctgatc 203 23 0 47.83 58.113 8.00 8.00 4.887 545 tgctagctaggcgatgctagct 411 22 0 54.55 62.888 9.00 8.00 4.888 546 agctagctgatcatcgatgctact 190 24 0 45.83 60.897 8.00 7.00 4.897 547 tcgatgctagtatgctagctagct 331 24 0 45.83 59.095 10.00 8.00 4.905 548 tgatcgatcgatgtgcgg 80 18 0 55.56 62.906 10.00 1.00 4.906 549 tcatctctgcgcgatcga 363 18 0 55.56 62.924 6.00 4.00 4.924 550 cggcgatctagctagctgactag 582 23 0 56.52 61.940 8.00 8.00 4.940 551 atgcatgctactagctagctgac 455 23 0 47.83 58.052 8.00 6.00 4.948 552 tagctagctgactagctgatcgat 590 24 0 45.83 59.042 9.00 6.00 4.958 553 tagctagctagctgactgatcgat 103 24 0 45.83 59.042 12.00 6.00 4.958 554 gatcgtagcggcgatctagcta 574 22 0 54.55 62.965 8.00 6.00 4.965 555 gatcgatgcatgcatgctactag 446 23 0 47.83 62.001 10.00 6.00 5.001 556 agctagctactgatcgatgctacat 303 25 0 44.00 60.001 8.00 4.00 5.001 557 gctatcgatcatcgatgctagct 543 23 0 47.83 62.017 11.00 8.00 5.017 558 atgcatgctactagctagctgact 455 24 0 45.83 58.980 8.00 6.00 5.020 559 catcggattagctagctgatgct 5 23 0 47.83 62.021 10.00 8.00 5.021 560 agcatcggattagctagctgatg 3 23 0 47.83 62.021 10.00 8.00 5.021 561 tttagctagctgactgatcgatc 257 23 0 43.48 57.951 8.00 8.00 5.049 562 gctatttagctagctgactgatcg 253 24 0 45.83 58.950 8.00 3.00 5.050 563 ctagctagctgactagctgatcg 589 23 0 52.17 57.942 9.00 3.00 5.058 564 cgatctagctagctgactagctg 585 23 0 52.17 57.942 9.00 7.00 5.058 565 ctagctagctagctgactgatcg 102 23 0 52.17 57.942 12.00 2.00 5.058 566 taggcgatgctagctagctacta 418 23 0 47.83 57.921 10.00 8.00 5.079 567 gctagctagtagctatcgatcatcg 532 25 0 48.00 60.083 11.00 7.00 5.083 568 gctagctagctactatcatcgatcg 426 25 0 48.00 60.083 10.00 6.00 5.083 569 atgctagtgatgcatgctagtagtg 23 25 0 44.00 59.907 8.00 4.00 5.093 570 ctagctagctgactagctgatcgat 589 25 0 48.00 59.895 9.00 6.00 5.105 571 atctagctagctgactagctgatcg 587 25 0 48.00 59.895 11.00 6.00 5.105 572 ctagctagctagctgactgatcgat 102 25 0 48.00 59.895 12.00 6.00 5.105 573 ctgactgatcgatcatcatgctag 266 24 0 45.83 61.155 12.00 6.00 5.155 574 tacatcgatcgatgctagtatgcta 323 25 0 40.00 60.165 12.00 6.00 5.165 575 ctaggcgatgctagctagctacta 417 24 0 50.00 58.803 10.00 8.00 5.197 576 tactatcatcgatcgatgcatgc 436 23 0 43.48 62.198 12.00 8.00 5.198 577 atcatcgatgctagctagctgatc 550 24 0 45.83 61.198 10.00 8.00 5.198 578 ctagctagctgatcatcatcgat 509 23 0 43.48 57.785 8.00 6.00 5.215 579 agctatttagctagctgactgatcg 252 25 0 44.00 59.785 8.00 5.00 5.215 580 tgcatgctactagctagctgactg 456 24 0 50.00 61.222 8.00 6.00 5.222 581 gctagtgatgcatgctagtagtga 25 24 0 45.83 58.772 8.00 2.00 5.228 582 tagctagctactgatcgatgctaca 302 25 0 44.00 59.772 10.00 2.00 5.228 583 tgactgatacgcgatgctagcta 474 23 0 47.83 62.247 6.00 6.00 5.247 584 tagctgactgatacgcgatgcta 470 23 0 47.83 62.247 4.00 2.00 5.247 585 atcgatcgatgctagtatgctag 326 23 0 43.48 57.749 10.00 8.00 5.251 586 gctactagctagctgactgatacgc 461 25 0 52.00 60.268 8.00 4.00 5.268 587 gcgatctagctagctgactagctg 584 24 0 54.17 61.268 9.00 7.00 5.268 588 tgatcatcatcgatgctagctagta 518 25 0 40.00 60.272 8.00 8.00 5.272 589 tgatcgatcatcatgctagctacta 271 25 0 40.00 60.272 10.00 4.00 5.272 590 ctgatacgcgatgctagctagct 477 23 0 52.17 62.283 10.00 8.00 5.283 591 atcatcatcgatgctagctagtagc 520 25 0 44.00 60.289 8.00 8.00 5.289 592 atcgatcatcatgctagctactagc 273 25 0 44.00 60.289 8.00 8.00 5.289 593 gatcatcatcgatgctagctagt 519 23 0 43.48 57.643 8.00 6.00 5.357 594 gatcgatcatcatgctagctact 272 23 0 43.48 57.643 8.00 2.00 5.357 595 tagctagctagctgatcatcgat 185 23 0 43.48 57.639 12.00 6.00 5.361 596 ctagctagctgactgatacgcgat 465 24 0 50.00 61.368 8.00 4.00 5.368 597 actagctagctgatcatcatcgat 508 24 0 41.67 58.612 8.00 6.00 5.388 598 agctgactagctgatcgatcgtag 595 24 0 50.00 61.392 8.00 8.00 5.392 599 ctagctgactagctgatcgatcgt 593 24 0 50.00 61.392 8.00 4.00 5.392 600 ctagctagctagctgatcatcgat 184 24 0 45.83 58.574 12.00 6.00 5.426 601 attagctagctgatgctagtgatgc 11 25 0 44.00 60.436 10.00 2.00 5.436 602 ggattagctagctgatgctagtg 9 23 0 47.83 57.556 10.00 4.00 5.444 603 ggattagctagctgatgctagtgat 9 25 0 44.00 59.520 10.00 7.00 5.480 604 agctagctactatcatcgatcga 429 23 0 43.48 57.502 8.00 8.00 5.498 605 gatcgatcgatgctagctagtag 120 23 0 47.83 57.502 10.00 6.00 5.498 606 gatcatcgatgctactagctagc 198 23 0 47.83 57.499 8.00 8.00 5.501 607 ctgatcgatcgatgctagctagg 399 23 0 52.17 62.515 10.00 6.00 5.515 608 tgctactagctagctgactgatacg 460 25 0 48.00 59.481 8.00 2.00 5.519 609 gctatttagctagctgactgatcga 253 25 0 44.00 60.527 8.00 4.00 5.527 610 tagctagctgatgctagtgatgca 13 24 0 45.83 61.529 8.00 4.00 5.529 611 gctactatcatctctgcgcgatc 356 23 0 52.17 62.545 4.00 4.00 5.545 612 ctagctagctagctgatcgatcga 387 24 0 50.00 61.552 12.00 8.00 5.552 613 ctagctagctagctgatcgatcga 67 24 0 50.00 61.552 12.00 8.00 5.552 614 atcgatgctactagctagctgatc 202 24 0 45.83 58.447 8.00 8.00 5.553 615 gatcatcgatgctactagctagct 198 24 0 45.83 58.447 8.00 8.00 5.553 616 cgatgctagtatgctagctagct 332 23 0 47.83 57.446 10.00 8.00 5.554 617 cgatgcatgcatgctactagcta 449 23 0 47.83 62.589 10.00 6.00 5.589 618 gcatgctactagctagctgactgat 457 25 0 48.00 60.594 8.00 4.00 5.594 619 atcgatgctagtatgctagctagct 330 25 0 44.00 59.362 10.00 8.00 5.638 620 gatgctagctagctgatcgatcg 556 23 0 52.17 62.647 10.00 6.00 5.647 621 tcgatcatcgatgctagctagct 547 23 0 47.83 62.647 10.00 8.00 5.647 622 gctgatcgatcgatgctagctag 398 23 0 52.17 62.647 10.00 8.00 5.647 623 agctgatcgatcgatgctagcta 397 23 0 47.83 62.647 12.00 8.00 5.647 624 tagctagctgatcgatcgatgct 392 23 0 47.83 62.647 10.00 8.00 5.647 625 ctagctagctgatcgatcgatgc 391 23 0 52.17 62.647 10.00 8.00 5.647 626 gctagctagctgatcgatcgatg 390 23 0 52.17 62.647 10.00 8.00 5.647 627 gctagctagctgatcgatcgatg 70 23 0 52.17 62.647 10.00 8.00 5.647 628 tactagctagctgatcatcatcga 507 24 0 41.67 58.348 8.00 7.00 5.652 629 tagctagctgactagctgatcgatc 590 25 0 48.00 60.659 9.00 8.00 5.659 630 tagctagctagctgactgatcgatc 103 25 0 48.00 60.659 12.00 8.00 5.659 631 tagctagctactatcatctctgcgc 350 25 0 48.00 60.659 10.00 4.00 5.659 632 gctagctagctactgatcgatgcta 300 25 0 48.00 60.659 10.00 6.00 5.659 633 gctagctagctgatcatcgatgc 187 23 0 52.17 62.663 10.00 6.00 5.663 634 atgctagctagctgatcgatcgta 557 24 0 45.83 61.663 10.00 6.00 5.663 635 tagctactatcatctctgcgcgat 354 24 0 45.83 61.663 6.00 4.00 5.663 636 tcgatcgatgctagtatgctagct 327 24 0 45.83 61.663 10.00 8.00 5.663 637 agctagctactatcatctctgcg 351 23 0 47.83 57.335 8.00 2.00 5.665 638 ctagctagctactgatcgatgct 301 23 0 47.83 57.335 10.00 4.00 5.665 639 agctagctagctactgatcgatg 299 23 0 47.83 57.335 12.00 4.00 5.665 640 cggattagctagctgatgctagtg 8 24 0 50.00 61.671 10.00 4.00 5.671 641 gctgatgctagtgatgcatgcta 19 23 0 47.83 62.674 6.00 6.00 5.674 642 tagctgatgctagtgatgcatgc 17 23 0 47.83 62.674 6.00 6.00 5.674 643 gactgatcgatcgatgctagctag 116 24 0 50.00 61.697 10.00 8.00 5.697 644 atttagctagctgactgatcgatc 256 24 0 41.67 58.295 8.00 8.00 5.705 645 atgcatgcatgctactagctagct 451 24 0 45.83 61.710 10.00 8.00 5.710 646 gatgctagtgatgcatgctagta 22 23 0 43.48 57.290 8.00 8.00 5.710 647 gatgctagtgatgcatgctagtag 22 24 0 45.83 58.286 8.00 6.00 5.714 648 tgctactagctagctgatcatcatc 207 25 0 44.00 59.265 8.00 7.00 5.735 649 gatgctactagctagctgatcatca 205 25 0 44.00 59.265 10.00 8.00 5.735 650 ctactagctagctgatcatcatcga 506 25 0 44.00 59.261 8.00 7.00 5.739 651 tttagctagctgactgatcgatcat 257 25 0 40.00 60.741 10.00 8.00 5.741 652 agctagctgatgctagtgatgcat 14 24 0 45.83 61.745 8.00 6.00 5.745 653 actgatcgatcgatgctagctagta 117 25 0 44.00 60.759 10.00 8.00 5.759 654 gctagctactgatcgatgctacatc 304 25 0 48.00 60.768 6.00 6.00 5.768 655 agctagctactgatcgatgctac 303 23 0 47.83 57.193 8.00 4.00 5.807 656 tgatgctagtgatgcatgctagtag 21 25 0 44.00 60.816 8.00 6.00 5.816 657 ctgatgctagtgatgcatgctagta 20 25 0 44.00 60.816 8.00 8.00 5.816 658 tgctactagctagctgatcatca 207 23 0 43.48 57.177 8.00 7.00 5.823 659 gctaggcgatgctagctagctact 416 24 0 54.17 61.851 10.00 6.00 5.851 660 agctaggcgatgctagctagctac 415 24 0 54.17 61.851 10.00 8.00 5.851 661 cgatcgatgctagtatgctagctag 328 25 0 48.00 60.861 10.00 8.00 5.861 662 ctgatcgatcgatgctagctagtag 118 25 0 48.00 60.867 10.00 6.00 5.867 663 actagctagctgactgatacgcga 464 24 0 50.00 61.869 8.00 3.00 5.869 664 gctactagctagctgatcatcatcg 505 25 0 48.00 60.876 8.00 5.00 5.876 665 tcgatgctactagctagctgatcat 203 25 0 44.00 60.876 8.00 8.00 5.876 666 atcgatgctactagctagctgatca 202 25 0 44.00 60.876 8.00 6.00 5.876 667 catcgatgctactagctagctgatc 201 25 0 48.00 60.876 8.00 8.00 5.876 668 tcatcgatgctactagctagctgat 200 25 0 44.00 60.876 9.00 8.00 5.876 669 gatcatcgatgctactagctagctg 198 25 0 48.00 60.876 8.00 7.00 5.876 670 tgatcatcgatgctactagctagct 197 25 0 44.00 60.876 8.00 8.00 5.876 671 ctgatcatcgatgctactagctagc 196 25 0 48.00 60.876 8.00 8.00 5.876 672 gctgatcatcgatgctactagctag 195 25 0 48.00 60.876 8.00 8.00 5.876 673 agctgatcatcgatgctactagcta 194 25 0 44.00 60.876 10.00 6.00 5.876 674 agctagctgatcatcgatgctacta 190 25 0 44.00 60.876 8.00 8.00 5.876 675 tagctagctgatcatcgatgctact 189 25 0 44.00 60.876 10.00 7.00 5.876 676 ctagctagctgatcatcgatgctac 188 25 0 48.00 60.876 10.00 8.00 5.876 677 agtgatgcatgctagtagtgatg 28 23 0 43.48 57.104 6.00 5.00 5.896 678 gctagtgatgcatgctagtagtgat 25 25 0 44.00 59.076 8.00 4.00 5.924 679 gatgctagtgatgcatgctagtagt 22 25 0 44.00 59.076 8.00 6.00 5.924 680 ctagctaggcgatgctagctagct 413 24 0 54.17 61.947 12.00 8.00 5.947 681 gctgatcatcatcgatgctagct 516 23 0 47.83 62.960 8.00 6.00 5.960 682 agctgatcatcatcgatgctagc 515 23 0 47.83 62.960 8.00 8.00 5.960 683 gctagctgatcatcatcgatgct 512 23 0 47.83 62.960 8.00 8.00 5.960 684 agctagctgatcatcatcgatgc 511 23 0 47.83 62.960 8.00 7.00 5.960 685 gactgatcgatcatcatgctagct 268 24 0 45.83 61.975 10.00 5.00 5.975 686 acatcgatcgatgctagtatgctag 324 25 0 44.00 60.976 12.00 8.00 5.976 687 ctagctagctagctgatcatcgatg 184 25 0 48.00 60.984 12.00 8.00 5.984 688 atgctagctaggcgatgctagct 410 23 0 52.17 62.989 9.00 8.00 5.989 689 actgatcgatcatcatgctagctac 269 25 0 44.00 60.996 10.00 4.00 5.996 690 agtgatgcatgctagtagtgatgt 28 24 0 41.67 57.997 6.00 3.00 6.003 691 tagctagctactgatcgatgctacat 302 26 0 42.31 60.010 10.00 4.00 6.010 692 ctactagctagctgactgatacgcg 462 25 0 52.00 61.048 8.00 4.00 6.048 693 atcggattagctagctgatgctagt 6 25 0 44.00 61.051 10.00 8.00 6.051 694 ctgatcatcatcgatgctagctagt 517 25 0 44.00 61.106 8.00 6.00 6.106 695 actagctagctgatcatcatcgatg 508 25 0 44.00 61.106 8.00 8.00 6.106 696 ctgatcgatcatcatgctagctact 270 25 0 44.00 61.106 10.00 2.00 6.106 697 gattagctagctgatgctagtgatg 10 25 0 44.00 58.837 10.00 5.00 6.163 698 tagctatttagctagctgactgatcg 251 26 0 42.31 59.800 10.00 4.00 6.200 699 tctagctagctgactagctgatcga 588 25 0 48.00 61.254 9.00 5.00 6.254 700 gatcatcatcgatgctagctagta 519 24 0 41.67 57.735 8.00 8.00 6.265 701 gatcgatcatcatgctagctacta 272 24 0 41.67 57.735 8.00 4.00 6.265 702 atgctactagctagctgactgatacg 459 26 0 46.15 59.732 8.00 2.00 6.268 703 tgctagctagctgatcgatcgtag 558 24 0 50.00 62.269 10.00 8.00 6.269 704 ctagctactatcatctctgcgcga 353 24 0 50.00 62.269 6.00 4.00 6.269 705 ctagctagctgactgatcgatcga 106 24 0 50.00 62.323 8.00 8.00 6.323 706 tgctagtgatgcatgctagtagtga 24 25 0 44.00 61.324 8.00 5.00 6.324 707 gatcatcatcgatgctagctagtag 519 25 0 44.00 58.669 8.00 6.00 6.331 708 tactagctagctgatcatcatcgat 507 25 0 40.00 58.669 8.00 6.00 6.331 709 gatcgatcatcatgctagctactag 272 25 0 44.00 58.669 8.00 6.00 6.331 710 tagctgactagctgatcgatcgtag 594 25 0 48.00 61.355 8.00 8.00 6.355 711 ctagctgactagctgatcgatcgta 593 25 0 48.00 61.355 8.00 6.00 6.355 712 atgcatgctactagctagctgactg 455 25 0 48.00 61.403 8.00 6.00 6.403 713 catgcatgctactagctagctgact 454 25 0 48.00 61.403 8.00 6.00 6.403 714 tagctagctactatcatcgatcga 428 24 0 41.67 57.596 10.00 8.00 6.404 715 ctactagctagctgatcatcatcg 506 24 0 45.83 57.570 8.00 5.00 6.430 716 ctgatcatcgatgctactagctag 196 24 0 45.83 57.570 8.00 8.00 6.430 717 tgctactagctagctgatcatcat 207 24 0 41.67 57.568 8.00 5.00 6.432 718 atgctactagctagctgatcatca 206 24 0 41.67 57.568 8.00 7.00 6.432 719 agctagctagctactgatcgatgct 299 25 0 48.00 61.440 12.00 4.00 6.440 720 atgctactagctagctgatcatcatc 206 26 0 42.31 59.532 8.00 7.00 6.468 721 ctactagctagctgatcatcatcgat 506 26 0 42.31 59.527 8.00 6.00 6.473 722 tgctagctagtagctatcgatcatc 531 25 0 44.00 58.507 11.00 5.00 6.493 723 tgctagctagctactatcatcgatc 425 25 0 44.00 58.507 10.00 4.00 6.493 724 ctagctagctactatcatcgatcga 427 25 0 44.00 58.506 10.00 8.00 6.494 725 gatgcatgcatgctactagctagc 450 24 0 50.00 62.511 10.00 8.00 6.511 726 ctagctagctgatcgatcgatgtg 71 24 0 50.00 62.543 10.00 8.00 6.543 727 tagctagctactatcatctctgcg 350 24 0 45.83 57.434 10.00 2.00 6.566 728 ctagctagctactgatcgatgcta 301 24 0 45.83 57.434 10.00 6.00 6.566 729 ctagctactgatcgatgctacatc 305 24 0 45.83 57.431 6.00 6.00 6.569 730 ctagctactgatcgatgctacatcg 305 25 0 48.00 61.570 8.00 8.00 6.570 731 ctagctagctactgatcgatgctaca 301 26 0 46.15 60.576 10.00 2.00 6.576 732 gcatgctactagctagctgactgata 457 26 0 46.15 60.584 8.00 4.00 6.584 733 tcgatcgatgctagtatgctagcta 327 25 0 44.00 61.618 10.00 8.00 6.618 734 atgctagtgatgcatgctagtagt 23 24 0 41.67 57.372 8.00 6.00 6.628 735 tatttagctagctgactgatcgatc 255 25 0 40.00 58.360 8.00 8.00 6.640 736 ctagctagctactatcatctctgcg 349 25 0 48.00 58.347 10.00 2.00 6.653 737 tagctagctgatgctagtgatgcat 13 25 0 44.00 61.696 8.00 6.00 6.696 738 tagctagctactgatcgatgctac 302 24 0 45.83 57.298 10.00 4.00 6.702 739 ctatttagctagctgactgatcga 254 24 0 41.67 57.276 8.00 4.00 6.724 740 gctatttagctagctgactgatcgat 253 26 0 42.31 60.726 8.00 6.00 6.726 741 catgctactagctagctgactgat 458 24 0 45.83 57.265 8.00 4.00 6.735 742 atcgatcatcgatgctagctagct 546 24 0 45.83 62.761 11.00 8.00 6.761 743 ctagctagctactgatcgatgctac 301 25 0 48.00 58.222 10.00 4.00 6.778 744 tagtgatgcatgctagtagtgatg 27 24 0 41.67 57.220 6.00 5.00 6.780 745 gatgcatgctagtagtgatgtatacg 31 26 0 42.31 59.217 7.00 4.00 6.783 746 ctatttagctagctgactgatcgatc 254 26 0 42.31 59.213 8.00 8.00 6.787 747 ctagtgatgcatgctagtagtgatg 26 25 0 44.00 58.212 8.00 5.00 6.788 748 tgcatgctagtagtgatgtatacg 33 24 0 41.67 57.201 6.00 4.00 6.799 749 gctaggcgatgctagctagctacta 416 25 0 52.00 61.803 10.00 8.00 6.803 750 tagctaggcgatgctagctagctac 414 25 0 52.00 61.803 10.00 8.00 6.803 751 tactagctagctgactgatacgcga 463 25 0 48.00 61.817 8.00 3.00 6.817 752 atcgatcgatgctagtatgctagct 326 25 0 44.00 61.817 10.00 8.00 6.817 753 attagctagctgatgctagtgatg 11 24 0 41.67 57.164 10.00 4.00 6.836 754 gatcgatgctagtatgctagctagct 329 26 0 46.15 60.851 10.00 8.00 6.851 755 gctgatcatcatcgatgctagcta 516 24 0 45.83 62.867 8.00 6.00 6.867 756 tagctgatcatcatcgatgctagc 514 24 0 45.83 62.867 8.00 8.00 6.867 757 gctagctgatcatcatcgatgcta 512 24 0 45.83 62.867 8.00 8.00 6.867 758 tagctagctgatcatcatcgatgc 510 24 0 45.83 62.867 8.00 7.00 6.867 759 actgatacgcgatgctagctagct 476 24 0 50.00 62.905 10.00 8.00 6.905 760 gactgatcgatcatcatgctagcta 268 25 0 44.00 61.916 10.00 6.00 6.916 761 agtgatgcatgctagtagtgatgta 28 25 0 40.00 58.077 6.00 5.00 6.923 762 tagtgatgcatgctagtagtgatgt 27 25 0 40.00 58.077 6.00 3.00 6.923 763 gtgatgcatgctagtagtgatgta 29 24 0 41.67 57.072 6.00 5.00 6.928 764 atttagctagctgactgatcgatcat 256 26 0 38.46 60.936 10.00 8.00 6.936 765 tacatcgatcgatgctagtatgctag 323 26 0 42.31 60.954 12.00 8.00 6.954 766 tgcatgctagtagtgatgtatacgt 33 25 0 40.00 58.033 6.00 6.00 6.967 767 ctactatcatcgatcgatgcatgc 435 24 0 45.83 62.969 12.00 8.00 6.969 768 ctactagctagctgactgatacgc 462 24 0 50.00 57.004 8.00 3.00 6.996 769 gctactagctagctgactgatacg 461 24 0 50.00 57.004 8.00 2.00 6.996 770 tgactgatacgcgatgctagctag 474 24 0 50.00 62.998 8.00 8.00 6.998 771 ctgactgatacgcgatgctagcta 473 24 0 50.00 62.998 6.00 6.00 6.998 772 tagctgactgatacgcgatgctag 470 24 0 50.00 62.998 4.00 4.00 6.998 773 ctagctgactgatacgcgatgcta 469 24 0 50.00 62.998 4.00 2.00 6.998 774 tagctagctgactgatacgcgatg 466 24 0 50.00 62.998 8.00 2.00 6.998 775 ctagtgatgcatgctagtagtgatgt 26 26 0 42.31 58.999 8.00 3.00 7.001 776 gctgatcatcatctagctagtagcttt 154 27 0 40.74 60.007 9.00 5.00 7.007 777 actagctagctgactgatacgcgat 464 25 0 48.00 62.015 8.00 4.00 7.015 778 atcgatgctactagctagctgatcat 202 26 0 42.31 61.064 8.00 8.00 7.064 779 gatgcatgctagtagtgatgtatacgt 31 27 0 40.74 59.925 7.00 6.00 7.075 780 atgctactagctagctgatcatcat 206 25 0 40.00 57.922 8.00 8.00 7.078 781 tgatcatcatcgatgctagctagtag 518 26 0 42.31 61.079 8.00 6.00 7.079 782 ctgatcatcatcgatgctagctagta 517 26 0 42.31 61.079 8.00 8.00 7.079 783 tactagctagctgatcatcatcgatg 507 26 0 42.31 61.079 8.00 8.00 7.079 784 tgatcgatcatcatgctagctactag 271 26 0 42.31 61.079 10.00 6.00 7.079 785 ctgatcgatcatcatgctagctacta 270 26 0 42.31 61.079 10.00 4.00 7.079 786 gctgatcatcatctagctagtagctt 154 26 0 42.31 58.900 9.00 7.00 7.100 787 atgctagctagtagctatcgatcatc 530 26 0 42.31 58.799 11.00 5.00 7.201 788 atgctagctagctactatcatcgatc 424 26 0 42.31 58.799 12.00 4.00 7.201 789 gcatgctagtagtgatgtatacgtagc 34 27 0 44.44 59.740 6.00 6.00 7.260 790 agctatttagctagctgactgatcga 252 26 0 42.31 61.281 8.00 4.00 7.281 791 agctgatcatcatctagctagtagctt 153 27 0 40.74 59.708 10.00 7.00 7.292 792 gctagctagctactgatcgatgctac 300 26 0 50.00 61.306 10.00 4.00 7.306 793 agctactagctagctgatcatcatc 504 25 0 44.00 57.687 10.00 7.00 7.313 794 agctgatcatcatctagctagtagc 153 25 0 44.00 57.687 10.00 7.00 7.313 795 gactgatcgatcgatgctagctagt 116 25 0 48.00 62.355 10.00 6.00 7.355 796 ctatttagctagctgactgatcgat 254 25 0 40.00 57.634 8.00 6.00 7.366 797 atgctagctagctgatcgatcgtag 557 25 0 48.00 62.397 10.00 8.00 7.397 798 ctagctactatcatctctgcgcgat 353 25 0 48.00 62.397 6.00 4.00 7.397 799 ctagctagctactatcatctctgcgc 349 26 0 50.00 61.404 10.00 4.00 7.404 800 gctagctagctactatcatctctgcg 348 26 0 50.00 61.404 10.00 2.00 7.404 801 agctagctagctactgatcgatgcta 299 26 0 46.15 61.404 12.00 6.00 7.404 802 atgcatgctagtagtgatgtatacg 32 25 0 40.00 57.577 7.00 4.00 7.423 803 ctagctagctgactagctgatcgatc 589 26 0 50.00 61.427 9.00 8.00 7.427 804 tctagctagctgactagctgatcgat 588 26 0 46.15 61.427 9.00 6.00 7.427 805 atctagctagctgactagctgatcga 587 26 0 46.15 61.427 11.00 5.00 7.427 806 ctagctagctagctgactgatcgatc 102 26 0 50.00 61.427 12.00 8.00 7.427 807 gctagctagctactatcatctctgc 348 25 0 48.00 57.554 10.00 2.00 7.446 808 gctagtgatgcatgctagtagtgatg 25 26 0 46.15 61.500 8.00 5.00 7.500 809 tgctagtgatgcatgctagtagtgat 24 26 0 42.31 61.500 8.00 4.00 7.500 810 atgctagtgatgcatgctagtagtga 23 26 0 42.31 61.500 8.00 4.00 7.500 811 gatgctagtgatgcatgctagtagtg 22 26 0 46.15 61.500 8.00 4.00 7.500 812 tgatgctagtgatgcatgctagtagt 21 26 0 42.31 61.500 8.00 6.00 7.500 813 actgatcgatcgatgctagctagtag 117 26 0 46.15 61.522 10.00 6.00 7.522 814 gtgatgcatgctagtagtgatgtat 29 25 0 40.00 57.469 7.00 7.00 7.531 815 agctagctactgatcgatgctacatc 303 26 0 46.15 61.533 8.00 6.00 7.533 816 agctaggcgatgctagctagctact 415 25 0 52.00 62.538 10.00 6.00 7.538 817 ctagctatttagctagctgactgatcg 250 27 0 44.44 60.549 12.00 6.00 7.549 818 gctagctagctactatcatcgatcga 426 26 0 46.15 61.570 10.00 8.00 7.570 819 agctagctagctactatcatctctgc 347 26 0 46.15 58.428 12.00 2.00 7.572 820 agtgatgcatgctagtagtgatgtat 28 26 0 38.46 58.410 7.00 7.00 7.590 821 ctgatgctagtgatgcatgctagtag 20 26 0 46.15 61.604 8.00 6.00 7.604 822 ttagctagctgatgctagtgatgca 12 25 0 44.00 62.607 8.00 4.00 7.607 823 agctactagctagctgatcatcatcg 504 26 0 46.15 61.633 10.00 5.00 7.633 824 ctgatcatcgatgctactagctagct 196 26 0 46.15 61.633 8.00 8.00 7.633 825 agctgatcatcgatgctactagctag 194 26 0 46.15 61.633 10.00 8.00 7.633 826 ctagctagctgatcatcgatgctact 188 26 0 46.15 61.633 10.00 7.00 7.633 827 catgctactagctagctgactgata 458 25 0 44.00 57.367 8.00 4.00 7.633 828 atgcatgctagtagtgatgtatacgt 32 26 0 38.46 58.359 7.00 6.00 7.641 829 catcggattagctagctgatgctag 5 25 0 48.00 62.679 10.00 8.00 7.679 830 tatcgatcatcgatgctagctagct 545 25 0 44.00 62.682 11.00 8.00 7.682 831 gctgatcatcatctactatcatcatca 219 27 0 37.04 60.705 8.00 8.00 7.705 832 atcgatcgatgctagtatgctagcta 326 26 0 42.31 61.770 10.00 8.00 7.770 833 ctagctagctactgatcgatgctacat 301 27 0 44.44 60.771 10.00 4.00 7.771 834 atgctagctagtagctatcgatcat 530 25 0 40.00 57.227 11.00 8.00 7.773 835 atgctagctagctactatcatcgat 424 25 0 40.00 57.227 12.00 6.00 7.773 836 actgatcgatcatcatgctagctact 269 26 0 42.31 61.773 10.00 3.00 7.773 837 gctgatcatcatctactatcatcatc 219 26 0 38.46 58.224 7.00 6.00 7.776 838 tgcatgctactagctagctgactga 456 25 0 48.00 62.811 8.00 4.00 7.811 839 gatcatcatcgatgctagctagtagc 519 26 0 46.15 61.816 8.00 8.00 7.816 840 gatcgatcatcatgctagctactagc 272 26 0 46.15 61.816 8.00 8.00 7.816 841 ggattagctagctgatgctagtgatg 9 26 0 46.15 61.822 10.00 5.00 7.822 842 catgctactagctagctgactgatac 458 26 0 46.15 58.154 8.00 2.00 7.846 843 tagtgatgcatgctagtagtgatgta 27 26 0 38.46 58.152 6.00 5.00 7.848 844 agctgatcatcatctactatcatcatc 218 27 0 37.04 59.122 9.00 6.00 7.878 845 tgcatgctagtagtgatgtatacgta 33 26 0 38.46 58.108 6.00 6.00 7.892 846 tatttagctagctgactgatcgatcat 255 27 0 37.04 60.916 10.00 8.00 7.916 847 agctgatcatcatctactatcatca 218 25 0 36.00 57.081 9.00 6.00 7.919 848 gattagctagctgatgctagtgatgc 10 26 0 46.15 61.942 10.00 5.00 7.942 849 ctagtgatgcatgctagtagtgatgta 26 27 0 40.74 59.041 8.00 5.00 7.959 850 tactagctagctgactgatacgcgat 463 26 0 46.15 61.961 8.00 4.00 7.961 851 tgcatgctagtagtgatgtatacgtag 33 27 0 40.74 58.973 6.00 4.00 8.027 852 gctagctgatcatcatctactatcatc 215 27 0 40.74 58.951 11.00 4.00 8.049 853 agctagctgatcatcatctactatca 214 26 0 38.46 57.910 11.00 6.00 8.090 854 ctagctgactagctgatcgatcgtag 593 26 0 50.00 62.099 8.00 8.00 8.099 855 tagctactagctagctgatcatcatc 503 26 0 42.31 57.771 11.00 7.00 8.229 856 tagctgatcatcatctagctagtagc 152 26 0 42.31 57.771 12.00 7.00 8.229 857 gcatgctactagctagctgactgatac 457 27 0 48.15 61.231 8.00 4.00 8.231 858 tagctatttagctagctgactgatcga 251 27 0 40.74 61.251 10.00 6.00 8.251 859 gactgatcgatcgatgctagctagta 116 26 0 46.15 62.288 10.00 8.00 8.288 860 tgctagctagtagctatcgatcatcg 531 26 0 46.15 62.331 11.00 7.00 8.331 861 tgctagctagctactatcatcgatcg 425 26 0 46.15 62.331 10.00 6.00 8.331 862 tcgatcgatgctagtatgctagctag 327 26 0 46.15 62.331 10.00 8.00 8.331 863 gctactagctagctgatcatcatcga 505 26 0 46.15 62.397 8.00 7.00 8.397 864 tcatcgatgctactagctagctgatc 200 26 0 46.15 62.397 9.00 8.00 8.397 865 tgatgcatgctagtagtgatgtatac 30 26 0 38.46 57.564 7.00 6.00 8.436 866 gtgatgcatgctagtagtgatgtata 29 26 0 38.46 57.564 7.00 5.00 8.436 867 agctatttagctagctgactgatcgat 252 27 0 40.74 61.441 8.00 6.00 8.441 868 agctaggcgatgctagctagctacta 415 26 0 50.00 62.471 10.00 8.00 8.471 869 tagctaggcgatgctagctagctact 414 26 0 50.00 62.471 10.00 6.00 8.471 870 ctagctaggcgatgctagctagctac 413 26 0 53.85 62.471 12.00 8.00 8.471 871 tagctagctactgatcgatgctacatc 302 27 0 44.44 61.494 10.00 6.00 8.494 872 agtgatgcatgctagtagtgatgtata 28 27 0 37.04 58.471 7.00 5.00 8.529 873 tagtgatgcatgctagtagtgatgtat 27 27 0 37.04 58.471 7.00 7.00 8.529 874 ctactagctagctgactgatacgcga 462 26 0 50.00 62.530 8.00 3.00 8.530 875 agctgatcatcatctactatcatcat 218 26 0 34.62 57.466 9.00 4.00 8.534 876 tgctactagctagctgactgatacgc 460 26 0 50.00 62.543 8.00 4.00 8.543 877 tgatcatcatctactatcatcatcatc 221 27 0 33.33 58.451 8.00 7.00 8.549 878 tgatgcatgctagtagtgatgtatacg 30 27 0 40.74 61.557 7.00 4.00 8.557 879 gactgatcgatcatcatgctagctac 268 26 0 46.15 62.566 10.00 4.00 8.566 880 atgcatgctagtagtgatgtatacgta 32 27 0 37.04 58.419 7.00 6.00 8.581 881 tcatctagctagtagctttatgctagc 162 27 0 40.74 58.416 11.00 6.00 8.584 882 atctagctagctgactagctgatcgat 587 27 0 44.44 61.585 11.00 7.00 8.585 883 tagctactagctagctgatcatcatcg 503 27 0 44.44 61.591 11.00 5.00 8.591 884 tagctgatcatcgatgctactagctag 193 27 0 44.44 61.591 12.00 8.00 8.591 885 gtgatgcatgctagtagtgatgtatac 29 27 0 40.74 58.348 7.00 7.00 8.652 886 atgctagtgatgcatgctagtagtgat 23 27 0 40.74 61.661 8.00 5.00 8.661 887 gctagctgatcatcatctactatcat 215 26 0 38.46 57.336 11.00 3.00 8.664 888 ctgatcatcatctactatcatcatca 220 26 0 34.62 57.322 8.00 8.00 8.678 889 ttagctagctgatgctagtgatgcat 12 26 0 42.31 62.718 8.00 6.00 8.718 890 attagctagctgatgctagtgatgca 11 26 0 42.31 62.718 10.00 4.00 8.718 891 actgatcgatcatcatgctagctacta 269 27 0 40.74 61.726 10.00 4.00 8.726 892 agctagctgatcatcatctactatcat 214 27 0 37.04 58.233 11.00 6.00 8.767 893 tagctgatcatcatctactatcatca 217 26 0 34.62 57.191 11.00 6.00 8.809 894 ctgatcatcatcgatgctagctagtag 517 27 0 44.44 61.823 8.00 6.00 8.823 895 ctactagctagctgatcatcatcgatg 506 27 0 44.44 61.823 8.00 8.00 8.823 896 ctgatcgatcatcatgctagctactag 270 27 0 44.44 61.823 10.00 6.00 8.823 897 ctagctgatcatcatctactatcatca 216 27 0 37.04 58.131 11.00 6.00 8.869 898 tgcatgctactagctagctgactgat 456 26 0 46.15 62.915 8.00 4.00 8.915 899 atgcatgctactagctagctgactga 455 26 0 46.15 62.915 8.00 4.00 8.915 900 catgctactagctagctgactgatacg 458 27 0 48.15 61.978 8.00 2.00 8.978 901 agctagctagctactgatcgatgctac 299 27 0 48.15 62.004 12.00 4.00 9.004 902 agctagctagctactatcatctctgcg 347 27 0 48.15 62.095 12.00 2.00 9.095 903 gctagtgatgcatgctagtagtgatgt 25 27 0 44.44 62.139 8.00 3.00 9.139 904 gctatttagctagctgactgatcgatc 253 27 0 44.44 62.155 8.00 8.00 9.155 905 ctgatgctagtgatgcatgctagtagt 20 27 0 44.44 62.235 8.00 6.00 9.235 906 catctagctagtagctttatgctagct 163 27 0 40.74 57.761 11.00 8.00 9.239 907 ctgatcatcatctactatcatcatcat 220 27 0 33.33 57.694 8.00 6.00 9.306 908 tagctaggcgatgctagctagctacta 414 27 0 48.15 62.409 10.00 8.00 9.409 909 tagctgatcatcatctactatcatcat 217 27 0 33.33 57.559 11.00 4.00 9.441 910 actagctatttagctagctgactgatc 249 27 0 40.74 57.556 12.00 6.00 9.444 911 atgctagctagtagctatcgatcatcg 530 27 0 44.44 62.447 11.00 7.00 9.447 912 atgctagctagctactatcatcgatcg 424 27 0 44.44 62.447 12.00 6.00 9.447 913 atcgatcgatgctagtatgctagctag 326 27 0 44.44 62.447 10.00 8.00 9.447 914 catgctagtagtgatgtatacgtagct 35 27 0 40.74 57.502 6.00 6.00 9.498 915 gctactagctagctgatcatcatcgat 505 27 0 44.44 62.514 8.00 6.00 9.514 916 atcatcgatgctactagctagctgatc 199 27 0 44.44 62.514 11.00 8.00 9.514 917 ctactagctagctgactgatacgcgat 462 27 0 48.15 62.638 8.00 4.00 9.638 918 atgctactagctagctgactgatacgc 459 27 0 48.15 62.652 8.00 4.00 9.652 919 attagctagctgatgctagtgatgcat 11 27 0 40.74 62.819 10.00 6.00 9.819 920 tgcatgctactagctagctgactgata 456 27 0 44.44 62.833 8.00 4.00 9.833 921 tctagctagctgactagctgatcgatc 588 27 0 48.15 62.883 9.00 8.00 9.883 922 gactgatcgatcgatgctagctagtag 116 27 0 48.15 62.974 10.00 6.00 9.974 923 ctgatcatcatctagctagtagcttta 155 27 0 37.04 57.019 9.00 7.00 9.981 primer3-2.3.6/test/primer1_list_last/included_test_w_middle.for0000664000077300007730000005577012231156243023710 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGTGTCTTGTGGGTGTGTCTG 79 21 0 52.38 60.680 1.00 1.00 1.680 1 TGTCTTGTGGGTGTGTCTGTT 81 21 0 47.62 59.059 1.00 0.00 1.941 2 GTGTCTTGTGGGTGTGTCTGTT 80 22 0 50.00 59.972 1.00 0.00 2.028 3 GAACATGTGTGGCATTGTGT 21 20 0 45.00 57.775 6.00 0.00 2.225 4 GTGTCTTGTGGGTGTGTCTGT 80 21 0 52.38 58.472 1.00 0.00 2.528 5 TGTCTTGTGGGTGTGTCTGT 81 20 0 50.00 57.431 1.00 0.00 2.569 6 GTGTCTTGTGGGTGTGTCTG 80 20 0 55.00 57.431 1.00 1.00 2.569 7 TGTGTCTTGTGGGTGTGTCT 79 20 0 50.00 57.431 1.00 0.00 2.569 8 TGTCTTGTGGGTGTGTCTGTTA 81 22 0 45.45 59.108 2.00 2.00 2.892 9 TTGTGGGTGTGTCTGTTAAACA 85 22 0 40.91 58.997 7.00 7.00 3.003 10 GTGTCTTGTGGGTGTGTCTGTTA 80 23 0 47.83 59.984 2.00 2.00 3.016 11 CTTGTGGGTGTGTCTGTTAAACA 84 23 0 43.48 59.981 7.00 7.00 3.019 12 GAACATGTGTGGCATTGTGTA 21 21 0 42.86 57.880 6.00 2.00 3.120 13 TGTCTTGTGGGTGTGTCTGTTAA 81 23 0 43.48 60.493 4.00 4.00 3.493 14 TGTGGGTGTGTCTGTTAAACA 86 21 0 42.86 57.489 7.00 7.00 3.511 15 TGTGTCTTGTGGGTGTGTCTGT 79 22 0 50.00 61.549 1.00 0.00 3.549 16 AAACATGTGTGGTATTGTGCA 102 21 0 38.10 57.391 6.00 4.00 3.609 17 TTGTGGGTGTGTCTGTTAAACAT 85 23 0 39.13 59.319 7.00 5.00 3.681 18 GAACATGTGTGGCATTGTGTAT 21 22 0 40.91 58.283 6.00 2.00 3.717 19 TGGGTGTGTCTGTTAAACATGTG 88 23 0 43.48 60.746 7.00 4.00 3.746 20 TGTGGGTGTGTCTGTTAAACATG 86 23 0 43.48 60.746 7.00 5.00 3.746 21 TCTTGTGGGTGTGTCTGTTAAA 83 22 0 40.91 58.187 4.00 3.00 3.813 22 AGCTTCTTGACTGTGTGTTGTG 149 22 0 45.45 58.075 4.00 0.00 3.925 23 TCTTGTGGGTGTGTCTGTTAAAC 83 23 0 43.48 59.063 5.00 5.00 3.937 24 GTCTTGTGGGTGTGTCTGTTAAA 82 23 0 43.48 59.063 4.00 3.00 3.937 25 TGGATGTAGCTTCTTGACTGTG 142 22 0 45.45 58.036 4.00 1.00 3.964 26 AGCTTCTTGACTGTGTGTTGTGT 149 23 0 43.48 58.981 4.00 0.00 4.019 27 GGATGTAGCTTCTTGACTGTGTG 143 23 0 47.83 58.925 4.00 0.00 4.075 28 TGGATGTAGCTTCTTGACTGTGT 142 23 0 43.48 58.925 4.00 1.00 4.075 29 TTAAACATGTGTGGTATTGTGCA 100 23 0 34.78 58.925 6.00 4.00 4.075 30 GGGTGTGTCTGTTAAACATGTG 89 22 0 45.45 57.912 7.00 4.00 4.088 31 TGGGTGTGTCTGTTAAACATGT 88 22 0 40.91 57.912 7.00 6.00 4.088 32 GTGGGTGTGTCTGTTAAACATG 87 22 0 45.45 57.912 7.00 5.00 4.088 33 TGTGGGTGTGTCTGTTAAACAT 86 22 0 40.91 57.912 7.00 5.00 4.088 34 AACATGTGTGGCATTGTGTATACA 22 24 0 37.50 60.103 8.00 8.00 4.103 35 GTCTTGTGGGTGTGTCTGTTAAAC 82 24 0 45.83 59.875 5.00 5.00 4.125 36 CATGTGTGGCATTGTGTATACA 24 22 0 40.91 57.850 8.00 8.00 4.150 37 AAACATGTGTGGTATTGTGCAT 102 22 0 36.36 57.811 6.00 2.00 4.189 38 GGGTGTGTCTGTTAAACATGTGT 89 23 0 43.48 58.808 7.00 6.00 4.192 39 GTGGGTGTGTCTGTTAAACATGT 87 23 0 43.48 58.808 7.00 6.00 4.192 40 ACATGTGTGGCATTGTGTATACA 23 23 0 39.13 58.766 8.00 8.00 4.234 41 CTTGTGGGTGTGTCTGTTAAACAT 84 24 0 41.67 60.239 7.00 5.00 4.239 42 GGATGTAGCTTCTTGACTGTGTGT 143 24 0 45.83 59.748 4.00 0.00 4.252 43 GTTAAACATGTGTGGTATTGTGCA 99 24 0 37.50 59.733 6.00 4.00 4.267 44 TGTTAAACATGTGTGGTATTGTGC 98 24 0 37.50 59.733 7.00 2.00 4.267 45 GTCTTGTGGGTGTGTCTGTTAA 82 22 0 45.45 57.635 4.00 4.00 4.365 46 TAAACATGTGTGGTATTGTGCA 101 22 0 36.36 57.504 6.00 4.00 4.496 47 TGTGTGTTGTGTATGGATGTAGC 129 23 0 43.48 58.499 2.00 2.00 4.501 48 TGTGTGTTGTGTATGGATGTAGCT 129 24 0 41.67 59.493 4.00 4.00 4.507 49 ATGGATGTAGCTTCTTGACTGTG 141 23 0 43.48 58.408 4.00 1.00 4.592 50 GAACATGTGTGGCATTGTGTATA 21 23 0 39.13 58.360 6.00 4.00 4.640 51 TTGTGCATATACATGTGTGTTGTG 116 24 0 37.50 59.345 6.00 2.00 4.655 52 ATGGATGTAGCTTCTTGACTGTGT 141 24 0 41.67 59.238 4.00 1.00 4.762 53 TTAAACATGTGTGGTATTGTGCAT 100 24 0 33.33 59.233 6.00 2.00 4.767 54 GAACATGTGTGGCATTGTGTATAC 21 24 0 41.67 59.208 6.00 6.00 4.792 55 CTTGTGGGTGTGTCTGTTAAAC 84 22 0 45.45 57.176 5.00 5.00 4.824 56 TAGCTTCTTGACTGTGTGTTGTG 148 23 0 43.48 58.160 4.00 0.00 4.840 57 TGTAGCTTCTTGACTGTGTGTTG 146 23 0 43.48 58.160 4.00 0.00 4.840 58 TGTGTTGTGTATGGATGTAGCTTC 131 24 0 41.67 59.091 4.00 4.00 4.909 59 TAGCTTCTTGACTGTGTGTTGTGT 148 24 0 41.67 59.030 4.00 0.00 4.970 60 GTAGCTTCTTGACTGTGTGTTGTG 147 24 0 45.83 59.030 4.00 0.00 4.970 61 TGTAGCTTCTTGACTGTGTGTTGT 146 24 0 41.67 59.030 4.00 0.00 4.970 62 TGTGTCTGTTAAACATGTGTGG 92 22 0 40.91 57.024 7.00 2.00 4.976 63 TGTGTTGTGTATGGATGTAGCTTCT 131 25 0 40.00 60.002 4.00 2.00 5.002 64 GTTAAACATGTGTGGTATTGTGCAT 99 25 0 36.00 59.991 6.00 2.00 5.009 65 TGTGTCTGTTAAACATGTGTGGT 92 23 0 39.13 57.983 7.00 0.00 5.017 66 GTGTGTCTGTTAAACATGTGTGG 91 23 0 43.48 57.983 7.00 2.00 5.017 67 GGTGTGTCTGTTAAACATGTGTG 90 23 0 43.48 57.983 7.00 4.00 5.017 68 TGTGCATATACATGTGTGTTGTG 117 23 0 39.13 57.923 6.00 2.00 5.077 69 AAACATGTGTGGTATTGTGCATA 102 23 0 34.78 57.903 6.00 4.00 5.097 70 TAAACATGTGTGGTATTGTGCAT 101 23 0 34.78 57.903 6.00 2.00 5.097 71 GTGTGTTGTGTATGGATGTAGCTTC 130 25 0 44.00 59.880 4.00 4.00 5.120 72 GTGTGTCTGTTAAACATGTGTGGT 91 24 0 41.67 58.868 7.00 0.00 5.132 73 TTGTGCATATACATGTGTGTTGTGT 116 25 0 36.00 60.151 6.00 0.00 5.151 74 ATGTGTGTTGTGTATGGATGTAGC 128 24 0 41.67 58.842 2.00 2.00 5.158 75 GTAGCTTCTTGACTGTGTGTTGTGT 147 25 0 44.00 59.835 4.00 0.00 5.165 76 TTGTGTATGGATGTAGCTTCTTGA 135 24 0 37.50 58.834 4.00 2.00 5.166 77 TGTGCATATACATGTGTGTTGTGT 117 24 0 37.50 58.828 6.00 0.00 5.172 78 ATGTGTGTTGTGTATGGATGTAGCT 128 25 0 40.00 59.772 4.00 4.00 5.228 79 GTGTCTTGTGGGTGTGTCTGTTAA 80 24 0 45.83 61.270 4.00 4.00 5.270 80 GATGTAGCTTCTTGACTGTGTGTTG 144 25 0 44.00 60.272 4.00 0.00 5.272 81 ACATGTGTGTTGTGTATGGATGT 126 23 0 39.13 57.646 6.00 0.00 5.354 82 ATTGTGCATATACATGTGTGTTGTG 115 25 0 36.00 59.637 6.00 2.00 5.363 83 TTGTGTATGGATGTAGCTTCTTGAC 135 25 0 40.00 59.615 4.00 1.00 5.385 84 GTTGTGTATGGATGTAGCTTCTTGA 134 25 0 40.00 59.615 4.00 2.00 5.385 85 TGTTGTGTATGGATGTAGCTTCTTG 133 25 0 40.00 60.458 4.00 2.00 5.458 86 ATGTAGCTTCTTGACTGTGTGTTG 145 24 0 41.67 58.520 4.00 0.00 5.480 87 GGTGTGTCTGTTAAACATGTGTGG 90 24 0 45.83 61.509 7.00 2.00 5.509 88 GGGTGTGTCTGTTAAACATGTGTG 89 24 0 45.83 61.509 7.00 4.00 5.509 89 TGGGTGTGTCTGTTAAACATGTGT 88 24 0 41.67 61.509 7.00 6.00 5.509 90 GTGGGTGTGTCTGTTAAACATGTG 87 24 0 45.83 61.509 7.00 4.00 5.509 91 TGTGGGTGTGTCTGTTAAACATGT 86 24 0 41.67 61.509 7.00 6.00 5.509 92 TATGGATGTAGCTTCTTGACTGTG 140 24 0 41.67 58.476 4.00 1.00 5.524 93 TGTATGGATGTAGCTTCTTGACTG 138 24 0 41.67 58.476 4.00 1.00 5.524 94 TGTGTATGGATGTAGCTTCTTGA 136 23 0 39.13 57.467 4.00 2.00 5.533 95 CTGTTAAACATGTGTGGTATTGTGC 97 25 0 40.00 60.599 7.00 2.00 5.599 96 TGGATGTAGCTTCTTGACTGTGTG 142 24 0 45.83 61.619 4.00 0.00 5.619 97 AACATGTGTGGCATTGTGTATAC 22 23 0 39.13 57.373 6.00 6.00 5.627 98 TGTGTATGGATGTAGCTTCTTGAC 136 24 0 41.67 58.339 4.00 1.00 5.661 99 ATGTAGCTTCTTGACTGTGTGTTGT 145 25 0 40.00 59.333 4.00 0.00 5.667 100 TGGTATTGTGCATATACATGTGTG 111 24 0 37.50 58.315 6.00 4.00 5.685 101 TGTGGTATTGTGCATATACATGTG 109 24 0 37.50 58.315 6.00 4.00 5.685 102 TGTGTGGTATTGTGCATATACATG 107 24 0 37.50 58.315 6.00 6.00 5.685 103 CATGTGTGGTATTGTGCATATACA 105 24 0 37.50 58.315 6.00 6.00 5.685 104 TATGGATGTAGCTTCTTGACTGTGT 140 25 0 40.00 59.274 4.00 1.00 5.726 105 GTATGGATGTAGCTTCTTGACTGTG 139 25 0 44.00 59.274 4.00 1.00 5.726 106 TGTATGGATGTAGCTTCTTGACTGT 138 25 0 40.00 59.274 4.00 3.00 5.726 107 GTGTATGGATGTAGCTTCTTGACTG 137 25 0 44.00 59.274 4.00 1.00 5.726 108 TGTGTATGGATGTAGCTTCTTGACT 136 25 0 40.00 59.274 4.00 1.00 5.726 109 AGCTTCTTGACTGTGTGTTGTGTG 149 24 0 45.83 61.730 4.00 0.00 5.730 110 TTAAACATGTGTGGTATTGTGCATA 100 25 0 32.00 59.268 6.00 4.00 5.732 111 TCTTGTGGGTGTGTCTGTTAAACA 83 24 0 41.67 61.733 7.00 7.00 5.733 112 TGTCTTGTGGGTGTGTCTGTTAAA 81 24 0 41.67 61.733 4.00 3.00 5.733 113 AAACATGTGTGGTATTGTGCATAT 102 24 0 33.33 58.262 6.00 4.00 5.738 114 TGTGTGTTGTGTATGGATGTAGCTT 129 25 0 40.00 60.742 4.00 2.00 5.742 115 TGTGTTGTGTATGGATGTAGCTT 131 23 0 39.13 57.257 4.00 2.00 5.743 116 TGTGTCTTGTGGGTGTGTCTGTT 79 23 0 47.83 62.831 1.00 0.00 5.831 117 GTGTGTTGTGTATGGATGTAGCTT 130 24 0 41.67 58.137 4.00 2.00 5.863 118 GCATATACATGTGTGTTGTGTATGG 120 25 0 40.00 59.136 6.00 3.00 5.864 119 TGGTATTGTGCATATACATGTGTGT 111 25 0 36.00 59.136 6.00 6.00 5.864 120 GTGGTATTGTGCATATACATGTGTG 110 25 0 40.00 59.136 6.00 4.00 5.864 121 TGTGGTATTGTGCATATACATGTGT 109 25 0 36.00 59.136 6.00 6.00 5.864 122 GTGTGGTATTGTGCATATACATGTG 108 25 0 40.00 59.136 6.00 4.00 5.864 123 TGTGTGGTATTGTGCATATACATGT 107 25 0 36.00 59.136 6.00 6.00 5.864 124 ACATGTGTGGTATTGTGCATATACA 104 25 0 36.00 59.136 6.00 6.00 5.864 125 TGTGTCTGTTAAACATGTGTGGTA 92 24 0 37.50 58.069 7.00 2.00 5.931 126 GTTAAACATGTGTGGTATTGTGC 99 23 0 39.13 57.060 6.00 2.00 5.940 127 GGATGTAGCTTCTTGACTGTGTGTT 143 25 0 44.00 60.962 4.00 0.00 5.962 128 TTGTGTATGGATGTAGCTTCTTG 135 23 0 39.13 57.036 4.00 2.00 5.964 129 TTGTGGGTGTGTCTGTTAAACATG 85 24 0 41.67 61.967 7.00 5.00 5.967 130 GTTAAACATGTGTGGTATTGTGCATA 99 26 0 34.62 60.001 6.00 4.00 6.001 131 TAAACATGTGTGGTATTGTGCATA 101 24 0 33.33 57.988 6.00 4.00 6.012 132 GTATGGATGTAGCTTCTTGACTGTGT 139 26 0 42.31 60.018 4.00 1.00 6.018 133 GTGTATGGATGTAGCTTCTTGACTGT 137 26 0 42.31 60.018 4.00 3.00 6.018 134 GTGTGTCTGTTAAACATGTGTGGTA 91 25 0 40.00 58.919 7.00 2.00 6.081 135 TCTGTTAAACATGTGTGGTATTGTG 96 25 0 36.00 58.912 7.00 0.00 6.088 136 TGTCTGTTAAACATGTGTGGTATTG 94 25 0 36.00 58.912 7.00 0.00 6.088 137 GTTGTGTATGGATGTAGCTTCTTG 134 24 0 41.67 57.904 4.00 2.00 6.096 138 TGTTGTGTATGGATGTAGCTTCTT 133 24 0 37.50 57.904 4.00 4.00 6.096 139 GTGGTATTGTGCATATACATGTGTGT 110 26 0 38.46 59.900 6.00 6.00 6.100 140 GTGTGGTATTGTGCATATACATGTGT 108 26 0 38.46 59.900 6.00 6.00 6.100 141 CATGTGTGTTGTGTATGGATGTAG 127 24 0 41.67 57.896 4.00 0.00 6.104 142 TGTGCATATACATGTGTGTTGTGTA 117 25 0 36.00 58.882 6.00 2.00 6.118 143 TTGTGCATATACATGTGTGTTGTGTA 116 26 0 34.62 60.156 6.00 2.00 6.156 144 ACATGTGTGTTGTGTATGGATGTAG 126 25 0 40.00 58.761 6.00 0.00 6.239 145 ACATGTGTGTTGTGTATGGATGTA 126 24 0 37.50 57.747 6.00 2.00 6.253 146 TACATGTGTGTTGTGTATGGATGT 125 24 0 37.50 57.747 6.00 0.00 6.253 147 GTGTTGTGTATGGATGTAGCTTCTT 132 25 0 40.00 58.712 4.00 4.00 6.288 148 GTCTGTTAAACATGTGTGGTATTGTG 95 26 0 38.46 59.680 7.00 0.00 6.320 149 TGTCTGTTAAACATGTGTGGTATTGT 94 26 0 34.62 59.680 7.00 0.00 6.320 150 GTGTCTGTTAAACATGTGTGGTATTG 93 26 0 38.46 59.680 7.00 0.00 6.320 151 TGTGTCTGTTAAACATGTGTGGTATT 92 26 0 34.62 59.680 7.00 1.00 6.320 152 TATTGTGCATATACATGTGTGTTGTG 114 26 0 34.62 59.659 6.00 2.00 6.341 153 GTTGTGTATGGATGTAGCTTCTTGAC 134 26 0 42.31 60.343 4.00 1.00 6.343 154 GGTATTGTGCATATACATGTGTGTTG 112 26 0 38.46 60.346 6.00 4.00 6.346 155 TGGTATTGTGCATATACATGTGTGTT 111 26 0 34.62 60.346 6.00 4.00 6.346 156 AACATGTGTGGTATTGTGCATATACA 103 26 0 34.62 60.346 6.00 6.00 6.346 157 ATGTGTGGTATTGTGCATATACATG 106 25 0 36.00 58.650 6.00 6.00 6.350 158 CATGTGTGGTATTGTGCATATACAT 105 25 0 36.00 58.650 6.00 4.00 6.350 159 GATGTAGCTTCTTGACTGTGTGTT 144 24 0 41.67 57.626 4.00 0.00 6.374 160 ATTGTGCATATACATGTGTGTTGTGT 115 26 0 34.62 60.393 6.00 0.00 6.393 161 TTGTGTATGGATGTAGCTTCTTGACT 135 26 0 38.46 60.455 4.00 1.00 6.455 162 TTAAACATGTGTGGTATTGTGCATAT 100 26 0 30.77 59.536 6.00 4.00 6.464 163 CATGTGTGTTGTGTATGGATGTAGC 127 25 0 44.00 61.472 4.00 2.00 6.472 164 TGTGTCTGTTAAACATGTGTGGTAT 92 25 0 36.00 58.424 7.00 2.00 6.576 165 ATGTGTGGTATTGTGCATATACATGT 106 26 0 34.62 59.420 6.00 6.00 6.580 166 ACATGTGTGGTATTGTGCATATACAT 104 26 0 34.62 59.420 6.00 4.00 6.580 167 GTGTTGTGTATGGATGTAGCTTCT 132 24 0 41.67 57.400 4.00 2.00 6.600 168 TGCATATACATGTGTGTTGTGTATG 119 25 0 36.00 58.378 6.00 5.00 6.622 169 AAACATGTGTGGTATTGTGCATATA 102 25 0 32.00 58.331 6.00 4.00 6.669 170 TAAACATGTGTGGTATTGTGCATAT 101 25 0 32.00 58.331 6.00 4.00 6.669 171 TAGCTTCTTGACTGTGTGTTGTGTG 148 25 0 44.00 61.678 4.00 0.00 6.678 172 TGTAGCTTCTTGACTGTGTGTTGTG 146 25 0 44.00 61.678 4.00 0.00 6.678 173 GTGTGTTGTGTATGGATGTAGCTTCT 130 26 0 42.31 60.727 4.00 2.00 6.727 174 TGTGTCTTGTGGGTGTGTCTGTTA 79 24 0 45.83 62.734 2.00 2.00 6.734 175 ATACATGTGTGTTGTGTATGGATG 124 24 0 37.50 57.241 6.00 0.00 6.759 176 GTGTGTCTGTTAAACATGTGTGGTAT 91 26 0 38.46 59.220 7.00 2.00 6.780 177 ATGGATGTAGCTTCTTGACTGTGTG 141 25 0 44.00 61.791 4.00 0.00 6.791 178 GAACATGTGTGGCATTGTGTATACA 21 25 0 40.00 61.810 8.00 8.00 6.810 179 GTGCATATACATGTGTGTTGTGTATG 118 26 0 38.46 59.190 6.00 5.00 6.810 180 TGTGCATATACATGTGTGTTGTGTAT 117 26 0 34.62 59.190 6.00 3.00 6.810 181 GCATATACATGTGTGTTGTGTATGGA 120 26 0 38.46 60.811 6.00 1.00 6.811 182 CTGTTAAACATGTGTGGTATTGTG 97 24 0 37.50 57.140 7.00 0.00 6.860 183 ATACATGTGTGTTGTGTATGGATGT 124 25 0 36.00 58.124 6.00 0.00 6.876 184 TCTTGTGGGTGTGTCTGTTAAACAT 83 25 0 40.00 61.898 7.00 5.00 6.898 185 AAACATGTGTGGTATTGTGCATATAC 102 26 0 34.62 59.095 6.00 6.00 6.905 186 ATGTGTGTTGTGTATGGATGTAGCTT 128 26 0 38.46 60.948 4.00 2.00 6.948 187 AACATGTGTGGTATTGTGCATATA 103 24 0 33.33 57.002 6.00 4.00 6.998 188 GATGTAGCTTCTTGACTGTGTGTTGT 144 26 0 42.31 61.001 4.00 0.00 7.001 189 CATGTGTGGTATTGTGCATATACATG 105 26 0 38.46 61.147 6.00 6.00 7.147 190 GGTATTGTGCATATACATGTGTGTT 112 25 0 36.00 57.851 6.00 4.00 7.149 191 AACATGTGTGGTATTGTGCATATAC 103 25 0 36.00 57.851 6.00 6.00 7.149 192 GTGTTGTGTATGGATGTAGCTTCTTG 132 26 0 42.31 61.157 4.00 2.00 7.157 193 TGTGTTGTGTATGGATGTAGCTTCTT 131 26 0 38.46 61.157 4.00 4.00 7.157 194 TACATGTGTGTTGTGTATGGATGTA 125 25 0 36.00 57.841 6.00 2.00 7.159 195 TACATGTGTGTTGTGTATGGATGTAG 125 26 0 38.46 58.815 6.00 0.00 7.185 196 GGTGTGTCTGTTAAACATGTGTGGT 90 25 0 44.00 62.217 7.00 0.00 7.217 197 GTGGGTGTGTCTGTTAAACATGTGT 87 25 0 44.00 62.217 7.00 6.00 7.217 198 GTTAAACATGTGTGGTATTGTGCATAT 99 27 0 33.33 60.227 6.00 4.00 7.227 199 CATATACATGTGTGTTGTGTATGGATG 121 27 0 37.04 60.230 6.00 0.00 7.230 200 TGTTAAACATGTGTGGTATTGTGCA 98 25 0 36.00 62.264 7.00 4.00 7.264 201 TGGATGTAGCTTCTTGACTGTGTGT 142 25 0 44.00 62.322 4.00 0.00 7.322 202 TTGTGCATATACATGTGTGTTGTGTAT 116 27 0 33.33 60.389 6.00 3.00 7.389 203 ATTGTGCATATACATGTGTGTTGTGTA 115 27 0 33.33 60.389 6.00 2.00 7.389 204 TATTGTGCATATACATGTGTGTTGTGT 114 27 0 33.33 60.389 6.00 0.00 7.389 205 GTATTGTGCATATACATGTGTGTTGTG 113 27 0 37.04 60.389 6.00 2.00 7.389 206 GTGTCTGTTAAACATGTGTGGTATTGT 93 27 0 37.04 60.396 7.00 0.00 7.396 207 GTGTGTCTGTTAAACATGTGTGGTATT 91 27 0 37.04 60.396 7.00 1.00 7.396 208 TGTTGTGTATAAATGTTGTGTCTTCA 52 26 0 30.77 58.596 4.00 1.00 7.404 209 GTCTTGTGGGTGTGTCTGTTAAACA 82 25 0 44.00 62.427 7.00 7.00 7.427 210 TGTCTTGTGGGTGTGTCTGTTAAAC 81 25 0 44.00 62.427 5.00 5.00 7.427 211 GTGTCTTGTGGGTGTGTCTGTTAAA 80 25 0 44.00 62.427 4.00 3.00 7.427 212 TTAAACATGTGTGGTATTGTGCATATA 100 27 0 29.63 59.560 6.00 4.00 7.440 213 AGCTTCTTGACTGTGTGTTGTGTGT 149 25 0 44.00 62.443 4.00 0.00 7.443 214 AACATGTGTGGTATTGTGCATATACAT 103 27 0 33.33 60.562 6.00 4.00 7.562 215 TAAACATGTGTGGTATTGTGCATATA 101 26 0 30.77 58.395 6.00 4.00 7.605 216 GTGTTGTGTATAAATGTTGTGTCTTCA 51 27 0 33.33 59.358 4.00 1.00 7.642 217 TGTGTTGTGTATAAATGTTGTGTCTTC 50 27 0 33.33 59.358 4.00 0.00 7.642 218 TTGTGGGTGTGTCTGTTAAACATGT 85 25 0 40.00 62.648 7.00 6.00 7.648 219 TATACATGTGTGTTGTGTATGGATG 123 25 0 36.00 57.352 6.00 0.00 7.648 220 CATATACATGTGTGTTGTGTATGGA 121 25 0 36.00 57.352 6.00 0.00 7.648 221 TGCATATACATGTGTGTTGTGTATGG 119 26 0 38.46 61.654 6.00 3.00 7.654 222 TGTGGTATTGTGCATATACATGTGTG 109 26 0 38.46 61.654 6.00 4.00 7.654 223 TGTGTGGTATTGTGCATATACATGTG 107 26 0 38.46 61.654 6.00 4.00 7.654 224 TATGGATGTAGCTTCTTGACTGTGTG 140 26 0 42.31 61.740 4.00 0.00 7.740 225 TGTATGGATGTAGCTTCTTGACTGTG 138 26 0 42.31 61.740 4.00 1.00 7.740 226 TGTGTATGGATGTAGCTTCTTGACTG 136 26 0 42.31 61.740 4.00 1.00 7.740 227 CTTGTGGGTGTGTCTGTTAAACATG 84 25 0 44.00 62.749 7.00 5.00 7.749 228 ATACATGTGTGTTGTGTATGGATGTA 124 26 0 34.62 58.200 6.00 2.00 7.800 229 TATACATGTGTGTTGTGTATGGATGT 123 26 0 34.62 58.200 6.00 0.00 7.800 230 ATGTAGCTTCTTGACTGTGTGTTGTG 145 26 0 42.31 61.844 4.00 0.00 7.844 231 GTCTGTTAAACATGTGTGGTATTGT 95 25 0 36.00 57.143 7.00 0.00 7.857 232 GTGTCTGTTAAACATGTGTGGTATT 93 25 0 36.00 57.143 7.00 1.00 7.857 233 TAAACATGTGTGGTATTGTGCATATAC 101 27 0 33.33 59.133 6.00 6.00 7.867 234 ATACATGTGTGTTGTGTATGGATGTAG 124 27 0 37.04 59.113 6.00 0.00 7.887 235 TATTGTGCATATACATGTGTGTTGT 114 25 0 32.00 57.073 6.00 2.00 7.927 236 GTATTGTGCATATACATGTGTGTTG 113 25 0 36.00 57.073 6.00 4.00 7.927 237 GCATATACATGTGTGTTGTGTATGGAT 120 27 0 37.04 61.010 6.00 2.00 8.010 238 GGTATTGTGCATATACATGTGTGTTGT 112 27 0 37.04 61.034 6.00 2.00 8.034 239 GTGGTATTGTGCATATACATGTGTGTT 110 27 0 37.04 61.034 6.00 4.00 8.034 240 TGTTGTGTATGGATGTAGCTTCTTGA 133 26 0 38.46 62.056 4.00 2.00 8.056 241 GTATTGTGCATATACATGTGTGTTGT 113 26 0 34.62 57.913 6.00 2.00 8.087 242 GTTGTGTATGGATGTAGCTTCTTGACT 134 27 0 40.74 61.126 4.00 1.00 8.126 243 GGTGTGTCTGTTAAACATGTGTGGTA 90 26 0 42.31 62.150 7.00 2.00 8.150 244 ACATGTGTGTTGTGTATGGATGTAGC 126 26 0 42.31 62.163 6.00 2.00 8.163 245 TCTGTTAAACATGTGTGGTATTGTGC 96 26 0 38.46 62.185 7.00 2.00 8.185 246 CATGTGTGTTGTGTATGGATGTAGCT 127 26 0 42.31 62.266 4.00 4.00 8.266 247 ATATACATGTGTGTTGTGTATGGATG 122 26 0 34.62 57.730 6.00 0.00 8.270 248 CATATACATGTGTGTTGTGTATGGAT 121 26 0 34.62 57.730 6.00 2.00 8.270 249 TGTGTTGTGTATAAATGTTGTGTCTT 50 26 0 30.77 57.700 4.00 0.00 8.300 250 TAGCTTCTTGACTGTGTGTTGTGTGT 148 26 0 42.31 62.367 4.00 0.00 8.367 251 GTAGCTTCTTGACTGTGTGTTGTGTG 147 26 0 46.15 62.367 4.00 0.00 8.367 252 TGTAGCTTCTTGACTGTGTGTTGTGT 146 26 0 42.31 62.367 4.00 0.00 8.367 253 TGTGTGTTGTGTATGGATGTAGCTTC 129 26 0 42.31 62.368 4.00 4.00 8.368 254 TGTTAAACATGTGTGGTATTGTGCAT 98 26 0 34.62 62.397 7.00 2.00 8.397 255 AAACATGTGTGGTATTGTGCATATACA 102 27 0 33.33 61.445 6.00 6.00 8.445 256 ATGGATGTAGCTTCTTGACTGTGTGT 141 26 0 42.31 62.454 4.00 0.00 8.454 257 ATATACATGTGTGTTGTGTATGGATGT 122 27 0 33.33 58.526 6.00 0.00 8.474 258 GTCTTGTGGGTGTGTCTGTTAAACAT 82 26 0 42.31 62.553 7.00 5.00 8.553 259 TGTGCATATACATGTGTGTTGTGTATG 117 27 0 37.04 61.688 6.00 5.00 8.688 260 TATACATGTGTGTTGTGTATGGATGTA 123 27 0 33.33 58.271 6.00 2.00 8.729 261 GTGTGTTGTGTATGGATGTAGCTTCTT 130 27 0 40.74 61.811 4.00 4.00 8.811 262 ATGTGTGGTATTGTGCATATACATGTG 106 27 0 37.04 61.814 6.00 4.00 8.814 263 CATGTGTGGTATTGTGCATATACATGT 105 27 0 37.04 61.814 6.00 6.00 8.814 264 ACATGTGTGGTATTGTGCATATACATG 104 27 0 37.04 61.814 6.00 6.00 8.814 265 ATGTGTTGTGTATAAATGTTGTGTCTT 49 27 0 29.63 58.041 4.00 0.00 8.959 266 TGTCTGTTAAACATGTGTGGTATTGTG 94 27 0 37.04 62.089 7.00 0.00 9.089 267 TGTGTCTGTTAAACATGTGTGGTATTG 92 27 0 37.04 62.089 7.00 0.00 9.089 268 TACATGTGTGTTGTGTATGGATGTAGC 125 27 0 40.74 62.100 6.00 2.00 9.100 269 GGTGTGTCTGTTAAACATGTGTGGTAT 90 27 0 40.74 62.286 7.00 2.00 9.286 270 GTGCATATACATGTGTGTTGTGTATGG 118 27 0 40.74 62.312 6.00 3.00 9.312 271 TGTGGTATTGTGCATATACATGTGTGT 109 27 0 37.04 62.312 6.00 6.00 9.312 272 GTGTGGTATTGTGCATATACATGTGTG 108 27 0 40.74 62.312 6.00 4.00 9.312 273 TGTGTGGTATTGTGCATATACATGTGT 107 27 0 37.04 62.312 6.00 6.00 9.312 274 TGTTAAACATGTGTGGTATTGTGCATA 98 27 0 33.33 62.329 7.00 4.00 9.329 275 TATGGATGTAGCTTCTTGACTGTGTGT 140 27 0 40.74 62.383 4.00 0.00 9.383 276 GTATGGATGTAGCTTCTTGACTGTGTG 139 27 0 44.44 62.383 4.00 0.00 9.383 277 TGTATGGATGTAGCTTCTTGACTGTGT 138 27 0 40.74 62.383 4.00 1.00 9.383 278 GTGTATGGATGTAGCTTCTTGACTGTG 137 27 0 44.44 62.383 4.00 1.00 9.383 279 TGTGTATGGATGTAGCTTCTTGACTGT 136 27 0 40.74 62.383 4.00 3.00 9.383 280 ATGTGTGTTGTGTATGGATGTAGCTTC 128 27 0 40.74 62.494 4.00 4.00 9.494 281 ATGTAGCTTCTTGACTGTGTGTTGTGT 145 27 0 40.74 62.495 4.00 0.00 9.495 282 TGTTGTGTATGGATGTAGCTTCTTGAC 133 27 0 40.74 62.685 4.00 1.00 9.685 283 GTGTTGTGTATGGATGTAGCTTCTTGA 132 27 0 40.74 62.685 4.00 2.00 9.685 284 TGGTATTGTGCATATACATGTGTGTTG 111 27 0 37.04 62.716 6.00 4.00 9.716 285 TTGTGTATGGATGTAGCTTCTTGACTG 135 27 0 40.74 62.778 4.00 1.00 9.778 286 GTCTGTTAAACATGTGTGGTATTGTGC 95 27 0 40.74 62.806 7.00 2.00 9.806 287 CATATGTGTTGTGTATAAATGTTGTGT 46 27 0 29.63 57.112 6.00 0.00 9.888 288 ACATATGTGTTGTGTATAAATGTTGTG 45 27 0 29.63 57.112 8.00 0.00 9.888 289 CACATATGTGTTGTGTATAAATGTTGT 44 27 0 29.63 57.112 10.00 2.00 9.888 290 ACACATATGTGTTGTGTATAAATGTTG 43 27 0 29.63 57.112 12.00 0.00 9.888 291 ACATGTGTGTTGTGTATGGATGTAGCT 126 27 0 40.74 62.902 6.00 4.00 9.902 primer3-2.3.6/test/primer1_list_last/SP04-07.txt.for0000664000077300007730000013025012231156243020731 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 CCCTACATACAGCCAAGGGA 22 20 0 55.00 59.948 4.00 0.00 0.052 1 TCCCTACATACAGCCAAGGG 21 20 0 55.00 59.948 4.00 1.00 0.052 2 CTAAGAATGTGGCCAAGGGA 123 20 0 50.00 60.066 6.00 0.00 0.066 3 TCCCTAAGAATGTGGCCAAG 120 20 0 50.00 60.066 6.00 2.00 0.066 4 GTTCCCTAAGAATGTGGCCA 118 20 0 50.00 59.933 6.00 6.00 0.067 5 TGTTCCCTAAGAATGTGGCC 117 20 0 50.00 59.933 4.00 4.00 0.067 6 GACTGCCACCGTGTACACAC 40 20 0 60.00 60.080 8.00 6.00 0.080 7 ACATACAGCCAAGGGACTGC 26 20 0 55.00 60.142 3.00 2.00 0.142 8 CCGCCATGTACACACTGAAG 96 20 0 55.00 60.175 6.00 2.00 0.175 9 TAAGAATGTGGCCAAGGGAA 124 20 0 45.00 60.439 6.00 0.00 0.439 10 TTCCCTAAGAATGTGGCCAA 119 20 0 45.00 60.439 6.00 4.00 0.439 11 GCCACCGTGTACACACTGAA 44 20 0 55.00 60.639 8.00 4.00 0.639 12 AAGAATGTGGCCAAGGGAAT 125 20 0 45.00 60.693 6.00 2.00 0.693 13 GAAATGTGGTTAAGGGACCG 76 20 0 50.00 59.288 4.00 2.00 0.712 14 TGGGTGTACCACACACTTCC 184 20 0 55.00 59.283 6.00 0.00 0.717 15 AATGCACTGAAGACCTTCCC 142 20 0 50.00 59.141 6.00 2.00 0.859 16 GGGAATGCACTGAAGACCTT 139 20 0 50.00 59.141 4.00 4.00 0.859 17 AAGGGAATGCACTGAAGACC 137 20 0 50.00 59.141 4.00 3.00 0.859 18 ACTGCCACCGTGTACACACT 41 20 0 55.00 59.101 8.00 6.00 0.899 19 GGACTGCCACCGTGTACAC 39 19 0 63.16 60.021 8.00 8.00 1.021 20 ATACAGCCAAGGGACTGCC 28 19 0 57.89 60.088 3.00 3.00 1.088 21 AAATGTGGTTAAGGGACCGC 77 20 0 50.00 61.099 4.00 2.00 1.099 22 TCCTACAGCTGTGGGAAAATG 201 21 0 47.62 60.118 12.00 0.00 1.118 23 AATGCACTGAAGACCTTCCCT 142 21 0 47.62 60.125 6.00 4.00 1.125 24 AGGGAATGCACTGAAGACCTT 138 21 0 47.62 60.125 4.00 4.00 1.125 25 AAGGGAATGCACTGAAGACCT 137 21 0 47.62 60.125 4.00 2.00 1.125 26 TGCACTGAAGACCTTCCCTAA 144 21 0 47.62 59.861 6.00 1.00 1.139 27 TACATACAGCCAAGGGACTGC 25 21 0 52.38 60.147 3.00 2.00 1.147 28 CCAAGGGAATGCACTGAAGA 135 20 0 50.00 61.176 4.00 0.00 1.176 29 GTGGGTGTACCACACACTTCC 183 21 0 57.14 60.189 8.00 0.00 1.189 30 AATGTGGTTAAGGGACCGC 78 19 0 52.63 59.805 4.00 2.00 1.195 31 ATGTTCCCTAAGAATGTGGCC 116 21 0 47.62 60.201 4.00 4.00 1.201 32 TGCCACCGTGTACACACTG 43 19 0 57.89 60.217 8.00 8.00 1.217 33 AGAAATGTGGTTAAGGGACCG 75 21 0 47.62 60.228 4.00 2.00 1.228 34 ATGCACTGAAGACCTTCCCT 143 20 0 50.00 58.744 6.00 4.00 1.256 35 AGGGAATGCACTGAAGACCT 138 20 0 50.00 58.744 4.00 2.00 1.256 36 CTGCCACCGTGTACACACTG 42 20 0 60.00 61.274 8.00 8.00 1.274 37 CATTGTGGGTGTACCACACA 179 20 0 50.00 58.674 8.00 8.00 1.326 38 TGGGTGTACCACACACTTCCT 184 21 0 52.38 60.328 6.00 0.00 1.328 39 CATTGTGGGTGTACCACACAC 179 21 0 52.38 59.632 8.00 8.00 1.368 40 ACATTGTGGGTGTACCACACA 178 21 0 47.62 59.632 8.00 8.00 1.368 41 CCGTGTACACACTGAACACGT 48 21 0 52.38 59.573 9.00 7.00 1.427 42 AAGAATGTGGCCAAGGGAA 125 19 0 47.37 60.445 6.00 0.00 1.445 43 CACGTTCCCTAAGAAATGTGG 64 21 0 47.62 59.480 8.00 6.00 1.520 44 CCACCGTGTACACACTGAACA 45 21 0 52.38 60.530 8.00 5.00 1.530 45 TGCACTGAAGACCTTCCCTA 144 20 0 50.00 58.442 6.00 2.00 1.558 46 AGAATGTGGCCAAGGGAAT 126 19 0 47.37 59.351 6.00 2.00 1.649 47 TGTGGGTGTACCACACACTTC 182 21 0 52.38 59.347 8.00 2.00 1.653 48 TAAGAATGTGGCCAAGGGAAT 124 21 0 42.86 60.678 6.00 2.00 1.678 49 CAAGGGAATGCACTGAAGAC 136 20 0 50.00 58.287 4.00 1.00 1.713 50 CGCCATGTACACACTGAAGA 97 20 0 50.00 58.276 6.00 1.00 1.724 51 CCTAAGAATGTGGCCAAGGG 122 20 0 55.00 61.732 6.00 1.00 1.732 52 CCCTAAGAATGTGGCCAAGG 121 20 0 55.00 61.732 6.00 2.00 1.732 53 CATACAGCCAAGGGACTGC 27 19 0 57.89 59.256 3.00 2.00 1.744 54 CCCTACATACAGCCAAGGGAC 22 21 0 57.14 60.748 4.00 1.00 1.748 55 CCAAGGGAATGCACTGAAG 135 19 0 52.63 59.226 4.00 2.00 1.774 56 GCCACCGTGTACACACTGA 44 19 0 57.89 59.114 8.00 6.00 1.886 57 GACTGCCACCGTGTACACA 40 19 0 57.89 59.114 8.00 6.00 1.886 58 CCGCCATGTACACACTGAA 96 19 0 52.63 59.097 6.00 1.00 1.903 59 TAAGAATGTGGCCAAGGGA 124 19 0 47.37 59.061 6.00 0.00 1.939 60 TCCCTAAGAATGTGGCCAA 120 19 0 47.37 59.061 6.00 4.00 1.939 61 TTCCCTAAGAATGTGGCCA 119 19 0 47.37 59.061 6.00 6.00 1.939 62 ACACACTGAACACGTTCCCT 54 20 0 50.00 58.039 6.00 4.00 1.961 63 AGGGACTGCCACCGTGTAC 37 19 0 63.16 61.004 5.00 4.00 2.004 64 GAATGCACTGAAGACCTTCCC 141 21 0 52.38 61.014 6.00 2.00 2.014 65 GGAATGCACTGAAGACCTTCC 140 21 0 52.38 61.014 6.00 4.00 2.014 66 GGGAATGCACTGAAGACCTTC 139 21 0 52.38 61.014 6.00 6.00 2.014 67 GGGACTGCCACCGTGTAC 38 18 0 66.67 59.962 5.00 4.00 2.038 68 GACTGCCACCGTGTACACACT 40 21 0 57.14 61.098 8.00 6.00 2.098 69 AGGGACTGCCACCGTGTA 37 18 0 61.11 60.113 5.00 2.00 2.113 70 TGCACTGAAGACCTTCCCTAAT 144 22 0 45.45 60.130 6.00 2.00 2.130 71 ATGCACTGAAGACCTTCCCTAA 143 22 0 45.45 60.130 6.00 1.00 2.130 72 AATGCACTGAAGACCTTCCCTA 142 22 0 45.45 60.130 6.00 2.00 2.130 73 AGAATGTGGCCAAGGGAATG 126 20 0 50.00 62.168 6.00 0.00 2.168 74 TTGTGGGTGTACCACACACTT 181 21 0 47.62 58.817 8.00 5.00 2.183 75 AAGACATTGTGGGTGTACCACA 175 22 0 45.45 60.193 6.00 6.00 2.193 76 ATGCACTGAAGACCTTCCCTA 143 21 0 47.62 58.803 6.00 2.00 2.197 77 TACAGCCAAGGGACTGCC 29 18 0 61.11 59.787 3.00 3.00 2.213 78 GTTCCCTAAGAATGTGGCCAA 118 21 0 47.62 61.214 6.00 4.00 2.214 79 TTCCCTACATACAGCCAAGGG 20 21 0 52.38 61.221 4.00 1.00 2.221 80 AGACATTGTGGGTGTACCACA 176 21 0 47.62 58.765 6.00 6.00 2.235 81 GAATGTGGCCAAGGGAATG 127 19 0 52.63 61.265 6.00 0.00 2.265 82 CTTCCCTACATACAGCCAAGG 19 21 0 52.38 58.730 4.00 2.00 2.270 83 CACGTTCCCTAAGAAATGTGGT 64 22 0 45.45 60.275 8.00 1.00 2.275 84 ACACGTTCCCTAAGAAATGTGG 63 22 0 45.45 60.275 8.00 6.00 2.275 85 TTCCCTACATACAGCCAAGG 20 20 0 50.00 57.700 3.00 2.00 2.300 86 CACACTGAACACGTTCCCTAAG 55 22 0 50.00 59.696 6.00 3.00 2.304 87 TGCCACCGTGTACACACTGA 43 20 0 55.00 62.305 8.00 6.00 2.305 88 CACACTGAACACGTTCCCTAA 55 21 0 47.62 58.687 6.00 1.00 2.313 89 AAGGGACTGCCACCGTGTAC 36 20 0 60.00 62.314 5.00 4.00 2.314 90 GCCGCCATGTACACACTG 95 18 0 61.11 59.685 6.00 4.00 2.315 91 TGGGTGTACCACACACTTCCTA 184 22 0 50.00 60.326 6.00 2.00 2.326 92 AGACATTGTGGGTGTACCACAC 176 22 0 50.00 59.670 6.00 6.00 2.330 93 CTAAGAATGTGGCCAAGGGAA 123 21 0 47.62 61.331 6.00 0.00 2.331 94 TTCCCTAAGAATGTGGCCAAG 119 21 0 47.62 61.331 6.00 2.00 2.331 95 CGCCATGTACACACTGAAGAT 97 21 0 47.62 58.664 6.00 3.00 2.336 96 GACATTGTGGGTGTACCACAC 177 21 0 52.38 58.609 6.00 6.00 2.391 97 GACATTGTGGGTGTACCACA 177 20 0 50.00 57.608 6.00 6.00 2.392 98 ACACACTGAACACGTTCCCTAA 54 22 0 45.45 59.562 6.00 1.00 2.438 99 ACCGTGTACACACTGAACACGT 47 22 0 50.00 60.445 9.00 7.00 2.445 100 ACTTCCCTACATACAGCCAAGG 18 22 0 50.00 59.543 4.00 2.00 2.457 101 GATGTTCCCTAAGAATGTGGC 115 21 0 47.62 58.517 3.00 2.00 2.483 102 GCCACCGTGTACACACTGAAC 44 21 0 57.14 61.486 8.00 4.00 2.486 103 ACATTGTGGGTGTACCACACAC 178 22 0 50.00 60.510 8.00 8.00 2.510 104 AGATGTTCCCTAAGAATGTGGC 114 22 0 45.45 59.487 3.00 2.00 2.513 105 AAGGGACTGCCACCGTGTA 36 19 0 57.89 61.530 5.00 2.00 2.530 106 CCACCGTGTACACACTGAAC 45 20 0 55.00 57.400 8.00 3.00 2.600 107 TGCACTGAAGACCTTCCCT 144 19 0 52.63 58.364 6.00 4.00 2.636 108 CATTGTGGGTGTACCACACACT 179 22 0 50.00 60.644 8.00 8.00 2.644 109 TCACACTCACTGAAGACTTCCC 3 22 0 50.00 59.350 7.00 3.00 2.650 110 GGGTGTACCACACACTTCCT 185 20 0 55.00 57.315 5.00 0.00 2.685 111 AATGTGGCCAAGGGAATG 128 18 0 50.00 59.296 6.00 0.00 2.704 112 GAATGCACTGAAGACCTTCC 141 20 0 50.00 57.288 6.00 4.00 2.712 113 GGAATGCACTGAAGACCTTC 140 20 0 50.00 57.288 6.00 6.00 2.712 114 CACTGAACACGTTCCCTAAGAA 57 22 0 45.45 59.281 6.00 2.00 2.719 115 TCCTACAGCTGTGGGAAAAT 201 20 0 45.00 57.268 12.00 2.00 2.732 116 TCCCTACATACAGCCAAGGGA 21 21 0 52.38 61.748 5.00 0.00 2.748 117 TTGTGGGTGTACCACACACTTC 181 22 0 50.00 60.765 8.00 3.00 2.765 118 TGTGGGTGTACCACACACTT 182 20 0 50.00 57.229 8.00 5.00 2.771 119 TTGTGGGTGTACCACACACT 181 20 0 50.00 57.229 8.00 6.00 2.771 120 GCCGCCATGTACACACTGA 95 19 0 57.89 61.775 6.00 2.00 2.775 121 GCCAAGGGAATGCACTGA 134 18 0 55.56 60.784 4.00 1.00 2.784 122 CCTACATACAGCCAAGGGACT 23 21 0 52.38 58.214 3.00 1.00 2.786 123 CACACACTTCCTACAGCTGTGG 193 22 0 54.55 60.796 8.00 8.00 2.796 124 CCACACACTTCCTACAGCTGTG 192 22 0 54.55 60.796 8.00 6.00 2.796 125 CGTGTACACACTGAACACGTTC 49 22 0 50.00 59.186 9.00 6.00 2.814 126 ATTGTGGGTGTACCACACACTT 180 22 0 45.45 59.161 8.00 6.00 2.839 127 CTAAGAATGTGGCCAAGGG 123 19 0 52.63 58.159 6.00 1.00 2.841 128 CCTAAGAATGTGGCCAAGG 122 19 0 52.63 58.159 6.00 2.00 2.841 129 CCCTAAGAATGTGGCCAAG 121 19 0 52.63 58.159 6.00 2.00 2.841 130 CCTACATACAGCCAAGGGAC 23 20 0 55.00 57.143 3.00 1.00 2.857 131 CACACTGAACACGTTCCCTA 55 20 0 50.00 57.141 6.00 2.00 2.859 132 TGAACACGTTCCCTAAGAAATG 60 22 0 40.91 59.131 6.00 4.00 2.869 133 ACACACTGAACACGTTCCCTA 54 21 0 47.62 58.130 6.00 2.00 2.870 134 TACACACTGAACACGTTCCCT 53 21 0 47.62 58.130 6.00 4.00 2.870 135 CGTTCCCTAAGAAATGTGGTT 66 21 0 42.86 58.111 4.00 0.00 2.889 136 CTGCCACCGTGTACACACT 42 19 0 57.89 58.109 8.00 6.00 2.891 137 TGTACACACTGAACACGTTCCC 51 22 0 50.00 60.906 6.00 2.00 2.906 138 ATACAGCCAAGGGACTGCCA 28 20 0 55.00 62.908 3.00 3.00 2.908 139 CATACAGCCAAGGGACTGCC 27 20 0 60.00 62.908 3.00 3.00 2.908 140 CCTACATACAGCCAAGGGACTG 23 22 0 54.55 60.913 3.00 3.00 2.913 141 CCGTGTACACACTGAACACGTT 48 22 0 50.00 60.956 9.00 5.00 2.956 142 GGACTGCCACCGTGTACA 39 18 0 61.11 59.043 6.00 6.00 2.957 143 CAAGGGAATGCACTGAAGACC 136 21 0 52.38 61.957 4.00 3.00 2.957 144 CCAAGGGAATGCACTGAAGAC 135 21 0 52.38 61.957 4.00 1.00 2.957 145 GTACACACTGAACACGTTCCCT 52 22 0 50.00 59.041 6.00 4.00 2.959 146 CCCTACATACAGCCAAGGG 22 19 0 57.89 58.034 4.00 1.00 2.966 147 AGAATGTGGCCAAGGGAA 126 18 0 50.00 59.009 6.00 0.00 2.991 148 AAGAATGTGGCCAAGGGA 125 18 0 50.00 59.009 6.00 0.00 2.991 149 GTACACACTGAACACGTTCCC 52 21 0 52.38 57.973 6.00 2.00 3.027 150 CTGAACACGTTCCCTAAGAAATG 59 23 0 43.48 60.042 6.00 4.00 3.042 151 CTACATACAGCCAAGGGACTGC 24 22 0 54.55 61.052 3.00 2.00 3.052 152 ATGTGGTTAAGGGACCGCC 79 19 0 57.89 62.052 4.00 1.00 3.052 153 ACTGCCACCGTGTACACAC 41 19 0 57.89 57.937 8.00 6.00 3.063 154 ACGTTCCCTAAGAAATGTGGTT 65 22 0 40.91 58.937 6.00 0.00 3.063 155 CCCTAAGAAATGTGGTTAAGGG 70 22 0 45.45 58.926 4.00 4.00 3.074 156 GAACACGTTCCCTAAGAAATGTG 61 23 0 43.48 59.920 8.00 8.00 3.080 157 TGAACACGTTCCCTAAGAAATGT 60 23 0 39.13 59.920 6.00 6.00 3.080 158 ACACTGAACACGTTCCCTAAGAA 56 23 0 43.48 60.082 6.00 2.00 3.082 159 CCGCCATGTACACACTGAAGA 96 21 0 52.38 62.092 6.00 1.00 3.092 160 ACTGCCACCGTGTACACACTG 41 21 0 57.14 62.102 8.00 8.00 3.102 161 ACACACTTCCTACAGCTGTGG 194 21 0 52.38 57.875 8.00 8.00 3.125 162 CCACACACTTCCTACAGCTGT 192 21 0 52.38 57.875 8.00 8.00 3.125 163 ACCACACACTTCCTACAGCTG 191 21 0 52.38 57.875 6.00 6.00 3.125 164 CACTGAACACGTTCCCTAAGA 57 21 0 47.62 57.871 6.00 1.00 3.129 165 TGCACTGAAGACCTTCCCTAATA 144 23 0 43.48 60.136 6.00 3.00 3.136 166 GTGGGTGTACCACACACTTCCT 183 22 0 54.55 61.149 8.00 0.00 3.149 167 GCCAAGGGAATGCACTGAA 134 19 0 52.63 62.155 4.00 3.00 3.155 168 TAAGACATTGTGGGTGTACCACA 174 23 0 43.48 60.196 6.00 6.00 3.196 169 ACCACACACTTCCTACAGCTGT 191 22 0 50.00 58.799 8.00 8.00 3.201 170 ACACTGAACACGTTCCCTAAGA 56 22 0 45.45 58.769 6.00 1.00 3.231 171 ATTGTGGGTGTACCACACACT 180 21 0 47.62 57.686 8.00 8.00 3.314 172 GAAGACTTCCCTACATACAGCCA 14 23 0 47.83 59.666 7.00 0.00 3.334 173 TGAAGACTTCCCTACATACAGCC 13 23 0 47.83 59.666 7.00 1.00 3.334 174 TCACACTCACTGAAGACTTCCCT 3 23 0 47.83 60.337 7.00 5.00 3.337 175 ATCACACTCACTGAAGACTTCCC 2 23 0 47.83 59.659 7.00 3.00 3.341 176 AAGGGAATGCACTGAAGACCTT 137 22 0 45.45 61.364 4.00 4.00 3.364 177 ATGCACTGAAGACCTTCCC 143 19 0 52.63 57.630 6.00 2.00 3.370 178 GGGAATGCACTGAAGACCT 139 19 0 52.63 57.630 4.00 2.00 3.370 179 AGGGAATGCACTGAAGACC 138 19 0 52.63 57.630 4.00 3.00 3.370 180 ATGCACTGAAGACCTTCCCTAAT 143 23 0 43.48 60.373 6.00 2.00 3.373 181 CCACCGTGTACACACTGAACAC 45 22 0 54.55 61.374 8.00 7.00 3.374 182 ACGTTCCCTAAGAAATGTGGT 65 21 0 42.86 57.593 6.00 0.00 3.407 183 TACACACTGAACACGTTCCCTAA 53 23 0 43.48 59.588 6.00 1.00 3.412 184 AAGAAATGTGGTTAAGGGACCG 74 22 0 45.45 61.417 4.00 2.00 3.417 185 GCTAAGACATTGTGGGTGTACC 172 22 0 50.00 58.513 5.00 4.00 3.487 186 ACACACTGAACACGTTCCCTAAG 54 23 0 47.83 60.494 6.00 3.00 3.494 187 CTAAGAATGTGGCCAAGGGAAT 123 22 0 45.45 61.515 6.00 2.00 3.515 188 CACCGTGTACACACTGAACAC 46 21 0 52.38 57.482 8.00 7.00 3.518 189 AGCTAAGACATTGTGGGTGTACC 171 23 0 47.83 59.468 5.00 4.00 3.532 190 CGCCATGTACACACTGAAGATG 97 22 0 50.00 61.537 6.00 5.00 3.537 191 CACTGAACACGTTCCCTAAGAAA 57 23 0 43.48 60.550 6.00 4.00 3.550 192 CACACTCACTGAAGACTTCCCT 4 22 0 50.00 58.449 7.00 5.00 3.551 193 CCCTAAGAAATGTGGTTAAGGGA 70 23 0 43.48 60.553 4.00 0.00 3.553 194 TCCCTAAGAAATGTGGTTAAGGG 69 23 0 43.48 60.553 4.00 4.00 3.553 195 CAAAAGCTAAGACATTGTGGG 167 21 0 42.86 57.442 5.00 0.00 3.558 196 CGTTCCCTAAGAAATGTGGTTAA 66 23 0 39.13 59.441 4.00 4.00 3.559 197 GGGTGTACCACACACTTCCTA 185 21 0 52.38 57.434 5.00 2.00 3.566 198 AAGGGACTGCCACCGTGT 36 18 0 61.11 61.583 5.00 3.00 3.583 199 GCACTGAAGACCTTCCCTAAT 145 21 0 47.62 57.417 6.00 2.00 3.583 200 GACATTGTGGGTGTACCACACA 177 22 0 50.00 61.591 8.00 8.00 3.591 201 CCCTACATACAGCCAAGGGACT 22 22 0 54.55 61.601 4.00 1.00 3.601 202 AAATGTGGTTAAGGGACCG 77 19 0 47.37 57.380 4.00 2.00 3.620 203 CTACATACAGCCAAGGGACTG 24 21 0 52.38 57.376 3.00 3.00 3.624 204 GGGTGTACCACACACTTCCTAC 185 22 0 54.55 58.361 5.00 0.00 3.639 205 ATGTGGTTAAGGGACCGC 79 18 0 55.56 58.359 4.00 2.00 3.641 206 CACACTCACTGAAGACTTCCC 4 21 0 52.38 57.332 7.00 3.00 3.668 207 TGTTCCCTAAGAATGTGGCCA 117 21 0 47.62 62.668 6.00 6.00 3.668 208 AAAAGCTAAGACATTGTGGGTG 168 22 0 40.91 58.314 5.00 0.00 3.686 209 CAAAAGCTAAGACATTGTGGGT 167 22 0 40.91 58.314 5.00 0.00 3.686 210 ACAAAAGCTAAGACATTGTGGG 166 22 0 40.91 58.314 5.00 1.00 3.686 211 AAGATGTTCCCTAAGAATGTGGC 113 23 0 43.48 60.707 4.00 2.00 3.707 212 CAAGGGAATGCACTGAAGA 136 19 0 47.37 57.292 4.00 0.00 3.708 213 GAATGTGGCCAAGGGAAT 127 18 0 50.00 58.274 6.00 2.00 3.726 214 AAGACATTGTGGGTGTACCAC 175 21 0 47.62 57.273 5.00 4.00 3.727 215 CGCCGCCATGTACACACT 94 18 0 61.11 61.777 6.00 2.00 3.777 216 TACAGCCAAGGGACTGCCA 29 19 0 57.89 62.781 3.00 3.00 3.781 217 TACACACTGAACACGTTCCCTA 53 22 0 45.45 58.214 6.00 2.00 3.786 218 AAGACTTCCCTACATACAGCCAA 15 23 0 43.48 59.209 5.00 2.00 3.791 219 GAAATGTGGTTAAGGGACCGC 76 21 0 52.38 62.792 4.00 2.00 3.792 220 CGTTCCCTAAGAAATGTGGTTA 66 22 0 40.91 58.188 4.00 2.00 3.812 221 AACACGTTCCCTAAGAAATGTG 62 22 0 40.91 58.175 8.00 8.00 3.825 222 CGTGTACACACTGAACACGTT 49 21 0 47.62 57.155 9.00 4.00 3.845 223 ATGTGGCCAAGGGAATGC 129 18 0 55.56 61.869 6.00 2.00 3.869 224 TGTGGTTAAGGGACCGCC 80 18 0 61.11 61.876 4.00 1.00 3.876 225 AAAAGCTAAGACATTGTGGGTGT 168 23 0 39.13 59.123 5.00 0.00 3.877 226 ACAAAAGCTAAGACATTGTGGGT 166 23 0 39.13 59.123 5.00 1.00 3.877 227 GAATGCACTGAAGACCTTCCCT 141 22 0 50.00 61.884 6.00 4.00 3.884 228 AGGGAATGCACTGAAGACCTTC 138 22 0 50.00 61.884 6.00 6.00 3.884 229 TCCTACAGCTGTGGGAAAATGA 201 22 0 45.45 61.895 12.00 1.00 3.895 230 CCAAGGGAATGCACTGAA 135 18 0 50.00 58.095 4.00 3.00 3.905 231 CAAAAGCTAAGACATTGTGGGTG 167 23 0 43.48 60.909 5.00 0.00 3.909 232 GTACACACTGAACACGTTCCCTA 52 23 0 47.83 59.087 6.00 2.00 3.913 233 GATGTTCCCTAAGAATGTGGCC 115 22 0 50.00 61.913 4.00 4.00 3.913 234 AAGAAATGTGGTTAAGGGACC 74 21 0 42.86 57.076 4.00 3.00 3.924 235 TGTACACACTGAACACGTTCC 51 21 0 47.62 57.053 6.00 4.00 3.947 236 GTGTACACACTGAACACGTTCC 50 22 0 50.00 58.046 8.00 4.00 3.954 237 GCCATGTACACACTGAAGATGTT 98 23 0 43.48 59.046 6.00 4.00 3.954 238 GTTCCCTAAGAATGTGGCC 118 19 0 52.63 57.030 4.00 4.00 3.970 239 CTGAACACGTTCCCTAAGAAA 59 21 0 42.86 57.025 6.00 4.00 3.975 240 GAAGATGTTCCCTAAGAATGTGG 112 23 0 43.48 59.014 6.00 0.00 3.986 241 ATTGTGGGTGTACCACACACTTC 180 23 0 47.83 60.995 8.00 5.00 3.995 242 AAGACATTGTGGGTGTACCACAC 175 23 0 47.83 60.995 6.00 6.00 3.995 243 CACACTGAACACGTTCCCT 55 19 0 52.63 57.003 6.00 4.00 3.997 244 ACACGTTCCCTAAGAAATGTGGT 63 23 0 43.48 61.013 8.00 6.00 4.013 245 ACGTTCCCTAAGAAATGTGGTTA 65 23 0 39.13 58.983 6.00 2.00 4.017 246 TACCACACACTTCCTACAGCTG 190 22 0 50.00 57.969 6.00 6.00 4.031 247 GTTCCCTAAGAATGTGGCCAAG 118 22 0 50.00 62.039 6.00 2.00 4.039 248 CTTCCCTACATACAGCCAAGGG 19 22 0 54.55 62.041 4.00 1.00 4.041 249 AAGACTTCCCTACATACAGCCAAG 15 24 0 45.83 60.076 5.00 4.00 4.076 250 ACTGAACACGTTCCCTAAGAAA 58 22 0 40.91 57.921 6.00 4.00 4.079 251 GACTTCCCTACATACAGCCAAG 17 22 0 50.00 57.893 4.00 4.00 4.107 252 AGACTTCCCTACATACAGCCAA 16 22 0 45.45 57.893 3.00 2.00 4.107 253 AAGACTTCCCTACATACAGCCA 15 22 0 45.45 57.893 5.00 0.00 4.107 254 GGGTGTACCACACACTTCCTACA 185 23 0 52.17 61.117 5.00 1.00 4.117 255 TGGGTGTACCACACACTTCCTAC 184 23 0 52.17 61.117 6.00 0.00 4.117 256 GTGGGTGTACCACACACTTCCTA 183 23 0 52.17 61.117 8.00 2.00 4.117 257 AGACTTCCCTACATACAGCCAAG 16 23 0 47.83 58.861 4.00 4.00 4.139 258 TACCACACACTTCCTACAGCTGT 190 23 0 47.83 58.855 8.00 8.00 4.145 259 GTACCACACACTTCCTACAGCTG 189 23 0 52.17 58.855 6.00 6.00 4.145 260 TGTACCACACACTTCCTACAGCT 188 23 0 47.83 58.855 4.00 4.00 4.145 261 ACGTTCCCTAAGAAATGTGGTTAA 65 24 0 37.50 60.157 6.00 4.00 4.157 262 TGTACCACACACTTCCTACAGC 188 22 0 50.00 57.817 4.00 2.00 4.183 263 GCTAAGACATTGTGGGTGTACCA 172 23 0 47.83 61.184 5.00 2.00 4.184 264 AAAGCTAAGACATTGTGGGTGT 169 22 0 40.91 57.804 5.00 0.00 4.196 265 GACTTCCCTACATACAGCCAAGG 17 23 0 52.17 61.222 4.00 2.00 4.222 266 CCGCCATGTACACACTGAAGAT 96 22 0 50.00 62.253 6.00 3.00 4.253 267 CGTTCCCTAAGAAATGTGGTTAAG 66 24 0 41.67 60.268 4.00 2.00 4.268 268 GTGTACCACACACTTCCTACAGC 187 23 0 52.17 58.715 5.00 2.00 4.285 269 CACACTGAAGATGTTCCCTAAGAA 106 24 0 41.67 59.690 6.00 2.00 4.310 270 GTACCACACACTTCCTACAGCTGT 189 24 0 50.00 59.674 8.00 8.00 4.326 271 GTGTACCACACACTTCCTACAGCT 187 24 0 50.00 59.674 5.00 4.00 4.326 272 AATGCACTGAAGACCTTCCCTAA 142 23 0 43.48 61.327 6.00 1.00 4.327 273 TCACACTCACTGAAGACTTCCCTA 3 24 0 45.83 60.335 7.00 3.00 4.335 274 TGTACACACTGAAGATGTTCCCT 102 23 0 43.48 58.639 6.00 4.00 4.361 275 GTACACACTGAACACGTTCCCTAA 52 24 0 45.83 60.367 6.00 1.00 4.367 276 ATGCACTGAAGACCTTCCCTAATA 143 24 0 41.67 60.370 6.00 3.00 4.370 277 TAAGAAATGTGGTTAAGGGACCG 73 23 0 43.48 61.379 4.00 2.00 4.379 278 TTCCCTAAGAAATGTGGTTAAGG 68 23 0 39.13 58.619 4.00 4.00 4.381 279 GCCATGTACACACTGAAGATGT 98 22 0 45.45 57.601 6.00 4.00 4.399 280 TGTACACACTGAAGATGTTCCC 102 22 0 45.45 57.574 6.00 2.00 4.426 281 GGTGTACCACACACTTCCTACAG 186 23 0 52.17 58.570 5.00 2.00 4.430 282 ACATTGTGGGTGTACCACACACT 178 23 0 47.83 61.441 8.00 8.00 4.441 283 CACGTTCCCTAAGAAATGTGGTT 64 23 0 43.48 61.453 8.00 0.00 4.453 284 AACACGTTCCCTAAGAAATGTGG 62 23 0 43.48 61.453 8.00 6.00 4.453 285 CTAAGACATTGTGGGTGTACCA 173 22 0 45.45 57.543 5.00 2.00 4.457 286 TCCCTACATACAGCCAAGGGAC 21 22 0 54.55 62.458 5.00 1.00 4.458 287 CACACTGAACACGTTCCCTAAGA 55 23 0 47.83 61.476 6.00 1.00 4.476 288 GCACTGAAGACCTTCCCTAATA 145 22 0 45.45 57.520 6.00 3.00 4.480 289 CACTCACTGAAGACTTCCCTACA 6 23 0 47.83 58.519 7.00 1.00 4.481 290 CACACTCACTGAAGACTTCCCTA 4 23 0 47.83 58.519 7.00 3.00 4.481 291 TCCCTAAGAATGTGGCCA 120 18 0 50.00 57.514 6.00 6.00 4.486 292 TACACACTGAACACGTTCCCTAAG 53 24 0 45.83 60.486 6.00 3.00 4.486 293 GGTGTACCACACACTTCCTACA 186 22 0 50.00 57.509 5.00 1.00 4.491 294 GTGGCCAAGGGAATGCAC 131 18 0 61.11 62.504 6.00 2.00 4.504 295 CTGAAGACTTCCCTACATACAGCC 12 24 0 50.00 60.533 7.00 1.00 4.533 296 CTGAACACGTTCCCTAAGAAAT 59 22 0 40.91 57.436 6.00 2.00 4.564 297 CTAAGACATTGTGGGTGTACCAC 173 23 0 47.83 58.433 5.00 4.00 4.567 298 CCGCCATGTACACACTGA 96 18 0 55.56 57.425 6.00 2.00 4.575 299 ACCACACACTTCCTACAGCTGTG 191 23 0 52.17 61.580 8.00 6.00 4.580 300 ATCACACTCACTGAAGACTTCCCT 2 24 0 45.83 60.582 7.00 5.00 4.582 301 TAAGACATTGTGGGTGTACCAC 174 22 0 45.45 57.389 5.00 4.00 4.611 302 TACAAAAGCTAAGACATTGTGGG 165 23 0 39.13 58.382 5.00 1.00 4.618 303 CACACTGAAGATGTTCCCTAAGA 106 23 0 43.48 58.382 6.00 1.00 4.618 304 GGCCAAGGGAATGCACTG 133 18 0 61.11 62.628 4.00 3.00 4.628 305 TGGCCAAGGGAATGCACT 132 18 0 55.56 62.628 6.00 4.00 4.628 306 ACACTCACTGAAGACTTCCCTACA 5 24 0 45.83 59.361 7.00 1.00 4.639 307 CACACTCACTGAAGACTTCCCTAC 4 24 0 50.00 59.361 7.00 1.00 4.639 308 GTTCCCTAAGAAATGTGGTTAAGG 67 24 0 41.67 59.360 4.00 4.00 4.640 309 GCACTGAAGACCTTCCCTAATAC 145 23 0 47.83 58.358 6.00 1.00 4.642 310 CCTAAGAAATGTGGTTAAGGGA 71 22 0 40.91 57.332 4.00 0.00 4.668 311 TCCCTAAGAAATGTGGTTAAGG 69 22 0 40.91 57.332 4.00 4.00 4.668 312 AAGCTAAGACATTGTGGGTGTACC 170 24 0 45.83 60.673 5.00 4.00 4.673 313 GTGTACACACTGAACACGTTCCC 50 23 0 52.17 61.688 8.00 2.00 4.688 314 CATGTACACACTGAAGATGTTCCC 100 24 0 45.83 60.700 6.00 2.00 4.700 315 CCATGTACACACTGAAGATGTTCC 99 24 0 45.83 60.700 6.00 4.00 4.700 316 GAACACGTTCCCTAAGAAATGT 61 22 0 40.91 57.291 6.00 6.00 4.709 317 ACTGAACACGTTCCCTAAGAAAT 58 23 0 39.13 58.273 6.00 2.00 4.727 318 TGAAGATGTTCCCTAAGAATGTG 111 23 0 39.13 58.251 6.00 0.00 4.749 319 AAGATGTTCCCTAAGAATGTGG 113 22 0 40.91 57.236 4.00 0.00 4.764 320 CTGAACACGTTCCCTAAGAAATGT 59 24 0 41.67 60.767 6.00 6.00 4.767 321 ACTGAACACGTTCCCTAAGAAATG 58 24 0 41.67 60.767 6.00 4.00 4.767 322 CACTGAACACGTTCCCTAAGAAAT 57 24 0 41.67 60.767 6.00 2.00 4.767 323 CAAGGGAATGCACTGAAGACCT 136 22 0 50.00 62.786 4.00 2.00 4.786 324 ACACACTGAAGATGTTCCCTAAGA 105 24 0 41.67 59.212 6.00 1.00 4.788 325 ATGTTCCCTAAGAATGTGGCCA 116 22 0 45.45 62.791 6.00 6.00 4.791 326 CTGAAGATGTTCCCTAAGAATGTG 110 24 0 41.67 59.196 6.00 0.00 4.804 327 CACTGAAGATGTTCCCTAAGAATG 108 24 0 41.67 59.196 6.00 0.00 4.804 328 TGTACACACTGAACACGTTCCCT 51 23 0 47.83 61.806 6.00 4.00 4.806 329 TAAGAAATGTGGTTAAGGGACC 73 22 0 40.91 57.190 4.00 3.00 4.810 330 GAATGCACTGAAGACCTTCCCTA 141 23 0 47.83 61.828 6.00 2.00 4.828 331 AAAAGCTAAGACATTGTGGGTGTA 168 24 0 37.50 59.162 5.00 2.00 4.838 332 TACAAAAGCTAAGACATTGTGGGT 165 24 0 37.50 59.162 5.00 0.00 4.838 333 GCCATGTACACACTGAAGATGTTC 98 24 0 45.83 60.841 6.00 6.00 4.841 334 TGCACTGAAGACCTTCCC 144 18 0 55.56 57.158 6.00 2.00 4.842 335 CTAAGAAATGTGGTTAAGGGACC 72 23 0 43.48 58.153 4.00 3.00 4.847 336 CCTAAGAAATGTGGTTAAGGGAC 71 23 0 43.48 58.153 4.00 1.00 4.847 337 GCACTGAAGACCTTCCCTAATACA 145 24 0 45.83 60.860 6.00 0.00 4.860 338 TGCACTGAAGACCTTCCCTAATAC 144 24 0 45.83 60.860 6.00 1.00 4.860 339 GAAGACTTCCCTACATACAGCCAA 14 24 0 45.83 60.860 7.00 2.00 4.860 340 CAGCCAAGGGACTGCCAC 31 18 0 66.67 62.888 3.00 1.00 4.888 341 ACAGCCAAGGGACTGCCA 30 18 0 61.11 62.888 3.00 3.00 4.888 342 TTCCCTACATACAGCCAAGGGA 20 22 0 50.00 62.893 5.00 0.00 4.893 343 CATTGTGGGTGTACCACACACTT 179 23 0 47.83 61.918 8.00 6.00 4.918 344 TAAGACATTGTGGGTGTACCACAC 174 24 0 45.83 60.969 6.00 6.00 4.969 345 TAAGAATGTGGCCAAGGG 124 18 0 50.00 57.022 6.00 1.00 4.978 346 CCCTAAGAATGTGGCCAA 121 18 0 50.00 57.022 6.00 4.00 4.978 347 ATGTACACACTGAAGATGTTCCC 101 23 0 43.48 57.976 6.00 2.00 5.024 348 TGTACACACTGAAGATGTTCCCTAA 102 25 0 40.00 59.975 6.00 1.00 5.025 349 ATGTACACACTGAAGATGTTCCCT 101 24 0 41.67 58.969 6.00 4.00 5.031 350 ACTGAAGATGTTCCCTAAGAATGTG 109 25 0 40.00 59.947 6.00 0.00 5.053 351 CACTGAAGATGTTCCCTAAGAATGT 108 25 0 40.00 59.947 6.00 1.00 5.053 352 ACACTGAAGATGTTCCCTAAGAATG 107 25 0 40.00 59.947 6.00 0.00 5.053 353 CACACTGAAGATGTTCCCTAAGAAT 106 25 0 40.00 59.947 6.00 2.00 5.053 354 CACTGAAGACCTTCCCTAATACAAA 146 25 0 40.00 59.946 6.00 0.00 5.054 355 CTAAGACATTGTGGGTGTACCACA 173 24 0 45.83 61.086 6.00 6.00 5.086 356 GATCACACTCACTGAAGACTTCC 1 23 0 47.83 57.900 7.00 5.00 5.100 357 AAAGCTAAGACATTGTGGGTGTA 169 23 0 39.13 57.892 5.00 2.00 5.108 358 AAAAGCTAAGACATTGTGGGTGTAC 168 25 0 40.00 59.890 5.00 4.00 5.110 359 AATACAAAAGCTAAGACATTGTGGG 163 25 0 36.00 59.858 5.00 0.00 5.142 360 CACTCACTGAAGACTTCCCTACAT 6 24 0 45.83 58.854 7.00 2.00 5.146 361 GAAGACCTTCCCTAATACAAAAGC 150 24 0 41.67 58.814 6.00 2.00 5.186 362 CCTAAGAAATGTGGTTAAGGGACC 71 24 0 45.83 61.226 4.00 3.00 5.226 363 CCCTAAGAAATGTGGTTAAGGGAC 70 24 0 45.83 61.226 4.00 1.00 5.226 364 CACTGAAGACCTTCCCTAATACAA 146 24 0 41.67 58.759 6.00 0.00 5.241 365 ACACTGAACACGTTCCCTAAGAAA 56 24 0 41.67 61.267 6.00 4.00 5.267 366 CGCCATGTACACACTGAAGATGT 97 23 0 47.83 62.286 6.00 4.00 5.286 367 AAAGCTAAGACATTGTGGGTGTAC 169 24 0 41.67 58.699 5.00 4.00 5.301 368 TGTACACACTGAAGATGTTCCCTA 102 24 0 41.67 58.699 6.00 2.00 5.301 369 ATACAAAAGCTAAGACATTGTGGG 164 24 0 37.50 58.698 5.00 0.00 5.302 370 ACACTCACTGAAGACTTCCCTACAT 5 25 0 44.00 59.643 7.00 2.00 5.357 371 GACATTGTGGGTGTACCACACAC 177 23 0 52.17 62.364 8.00 8.00 5.364 372 GAAGACCTTCCCTAATACAAAAGCT 150 25 0 40.00 59.629 6.00 4.00 5.371 373 CACGTTCCCTAAGAAATGTGGTTA 64 24 0 41.67 61.414 8.00 2.00 5.414 374 ACACACTGAAGATGTTCCCTAAGAA 105 25 0 40.00 60.434 6.00 2.00 5.434 375 GATCACACTCACTGAAGACTTCCC 1 24 0 50.00 61.444 7.00 3.00 5.444 376 AGACATTGTGGGTGTACCACACA 176 23 0 47.83 62.479 8.00 8.00 5.479 377 AATGCACTGAAGACCTTCCCTAAT 142 24 0 41.67 61.502 6.00 2.00 5.502 378 TACCACACACTTCCTACAGCTGTG 190 24 0 50.00 61.532 8.00 6.00 5.532 379 TGTACCACACACTTCCTACAGCTG 188 24 0 50.00 61.532 6.00 6.00 5.532 380 TGAAGATGTTCCCTAAGAATGTGG 111 24 0 41.67 61.537 6.00 0.00 5.537 381 CACTGAAGACCTTCCCTAATACA 146 23 0 43.48 57.450 6.00 0.00 5.550 382 ATACAAAAGCTAAGACATTGTGGGT 164 25 0 36.00 59.432 5.00 1.00 5.568 383 ATCACACTCACTGAAGACTTCCCTA 2 25 0 44.00 60.571 7.00 3.00 5.571 384 CACTGAAGACTTCCCTACATACAGC 10 25 0 48.00 60.575 7.00 2.00 5.575 385 ACACACTGAAGATGTTCCCTAAG 105 23 0 43.48 57.416 6.00 3.00 5.584 386 CAAAAGCTAAGACATTGTGGGTGT 167 24 0 41.67 61.607 5.00 0.00 5.607 387 ACAAAAGCTAAGACATTGTGGGTG 166 24 0 41.67 61.607 5.00 0.00 5.607 388 AAGCTAAGACATTGTGGGTGTAC 170 23 0 43.48 57.388 5.00 4.00 5.612 389 TTCCCTAAGAAATGTGGTTAAGGG 68 24 0 41.67 61.631 4.00 4.00 5.631 390 AGATGTTCCCTAAGAATGTGGCC 114 23 0 47.83 62.687 4.00 4.00 5.687 391 TCCTACAGCTGTGGGAAAATGAG 201 23 0 47.83 62.697 12.00 0.00 5.697 392 ACTTCCCTACATACAGCCAAGGG 18 23 0 52.17 62.697 4.00 1.00 5.697 393 CGTGTACACACTGAACACGTTCC 49 23 0 52.17 62.750 9.00 4.00 5.750 394 CCGTGTACACACTGAACACGTTC 48 23 0 52.17 62.750 9.00 6.00 5.750 395 TGTACACACTGAACACGTTCCCTA 51 24 0 45.83 61.750 6.00 2.00 5.750 396 TACACACTGAAGATGTTCCCTAAGA 104 25 0 40.00 59.249 6.00 1.00 5.751 397 CTGAAGACTTCCCTACATACAGC 12 23 0 47.83 57.219 7.00 2.00 5.781 398 AAGACCTTCCCTAATACAAAAGC 151 23 0 39.13 57.202 4.00 2.00 5.798 399 GTGGGTGTACCACACACTTCCTAC 183 24 0 54.17 61.849 8.00 0.00 5.849 400 CATGTACACACTGAAGATGTTCC 100 23 0 43.48 57.126 6.00 4.00 5.874 401 CCATGTACACACTGAAGATGTTC 99 23 0 43.48 57.126 6.00 6.00 5.874 402 GCTAAGACATTGTGGGTGTACCAC 172 24 0 50.00 61.894 5.00 4.00 5.894 403 AAGACCTTCCCTAATACAAAAGCT 151 24 0 37.50 58.100 4.00 4.00 5.900 404 ACGTTCCCTAAGAAATGTGGTTAAG 65 25 0 40.00 60.930 6.00 2.00 5.930 405 ACTGAAGACTTCCCTACATACAGC 11 24 0 45.83 58.067 7.00 2.00 5.933 406 TGAAGACCTTCCCTAATACAAAA 149 23 0 34.78 57.052 6.00 1.00 5.948 407 ACACTGAAGATGTTCCCTAAGAA 107 23 0 39.13 57.049 6.00 2.00 5.951 408 GGGTGTACCACACACTTCCTACAG 185 24 0 54.17 61.959 5.00 2.00 5.959 409 ATGTACACACTGAAGATGTTCCCTA 101 25 0 40.00 59.015 6.00 2.00 5.985 410 AGCTAAGACATTGTGGGTGTACCA 171 24 0 45.83 62.000 5.00 2.00 6.000 411 AGACTTCCCTACATACAGCCAAGG 16 24 0 50.00 62.003 4.00 2.00 6.003 412 TGAAGACCTTCCCTAATACAAAAG 149 24 0 37.50 57.993 6.00 3.00 6.007 413 CTGAAGACCTTCCCTAATACAAAA 148 24 0 37.50 57.993 6.00 1.00 6.007 414 GTACACACTGAAGATGTTCCCTAAGA 103 26 0 42.31 59.993 6.00 1.00 6.007 415 TCACTGAAGACTTCCCTACATACA 9 24 0 41.67 57.947 7.00 0.00 6.053 416 ATGCACTGAAGACCTTCCCTAATAC 143 25 0 44.00 61.054 6.00 1.00 6.054 417 TCACACTCACTGAAGACTTCCCTAC 3 25 0 48.00 61.088 7.00 1.00 6.088 418 GGTGTACCACACACTTCCTACAGC 186 24 0 54.17 62.095 5.00 2.00 6.095 419 TCCCTAAGAAATGTGGTTAAGGGA 69 24 0 41.67 62.095 6.00 6.00 6.095 420 TCACTGAAGACTTCCCTACATACAG 9 25 0 44.00 58.904 7.00 2.00 6.096 421 CTCACTGAAGACTTCCCTACATACA 8 25 0 44.00 58.904 7.00 0.00 6.096 422 CACTCACTGAAGACTTCCCTACATA 6 25 0 44.00 58.904 7.00 2.00 6.096 423 TGAAGACCTTCCCTAATACAAAAGC 149 25 0 40.00 61.112 6.00 2.00 6.112 424 ACACGTTCCCTAAGAAATGTGGTT 63 24 0 41.67 62.116 8.00 4.00 6.116 425 AACACGTTCCCTAAGAAATGTGGT 62 24 0 41.67 62.116 8.00 8.00 6.116 426 CTAAGAAATGTGGTTAAGGGACCG 72 24 0 45.83 62.125 4.00 2.00 6.125 427 TAATACAAAAGCTAAGACATTGTGGG 162 26 0 34.62 59.872 5.00 0.00 6.128 428 CTGAAGACCTTCCCTAATACAAAAG 148 25 0 40.00 58.861 6.00 3.00 6.139 429 ACACACTGAACACGTTCCCTAAGA 54 24 0 45.83 62.186 6.00 1.00 6.186 430 TGAAGACTTCCCTACATACAGCCA 13 24 0 45.83 62.191 7.00 0.00 6.191 431 GTACACACTGAACACGTTCCCTAAG 52 25 0 48.00 61.201 6.00 3.00 6.201 432 ATGTACACACTGAAGATGTTCCCTAA 101 26 0 38.46 60.216 6.00 1.00 6.216 433 CTCACTGAAGACTTCCCTACATACAG 8 26 0 46.15 59.782 7.00 2.00 6.218 434 ACTGAAGACTTCCCTACATACAGCC 11 25 0 48.00 61.224 7.00 1.00 6.224 435 ACTGAAGACCTTCCCTAATACAAAA 147 25 0 36.00 58.743 6.00 1.00 6.257 436 GAAGATGTTCCCTAAGAATGTGGC 112 24 0 45.83 62.298 6.00 2.00 6.298 437 ACTCACTGAAGACTTCCCTACATACA 7 26 0 42.31 59.665 7.00 0.00 6.335 438 CACTCACTGAAGACTTCCCTACATAC 6 26 0 46.15 59.665 7.00 0.00 6.335 439 ACACTCACTGAAGACTTCCCTACATA 5 26 0 42.31 59.665 7.00 2.00 6.335 440 GACCTTCCCTAATACAAAAGCTAAGA 153 26 0 38.46 59.649 4.00 1.00 6.351 441 GAAGACCTTCCCTAATACAAAAGCTA 150 26 0 38.46 59.649 6.00 2.00 6.351 442 CCTTCCCTAATACAAAAGCTAAGACA 155 26 0 38.46 60.381 4.00 0.00 6.381 443 TGAACACGTTCCCTAAGAAATGTG 60 24 0 41.67 62.411 8.00 8.00 6.411 444 TACACACTGAAGATGTTCCCTAAGAA 104 26 0 38.46 60.429 6.00 2.00 6.429 445 ACTGAACACGTTCCCTAAGAAATGT 58 25 0 40.00 61.443 6.00 6.00 6.443 446 ACACTGAACACGTTCCCTAAGAAAT 56 25 0 40.00 61.443 6.00 2.00 6.443 447 ACTGAAGACCTTCCCTAATACAAAAG 147 26 0 38.46 59.556 6.00 3.00 6.444 448 AATGCACTGAAGACCTTCCCTAATA 142 25 0 40.00 61.463 6.00 3.00 6.463 449 ACTGAAGACCTTCCCTAATACAAA 147 24 0 37.50 57.525 6.00 0.00 6.475 450 TACACACTGAAGATGTTCCCTAAG 104 24 0 41.67 57.518 6.00 3.00 6.482 451 GTGTACACACTGAACACGTTCCCT 50 24 0 50.00 62.520 8.00 4.00 6.520 452 AATACAAAAGCTAAGACATTGTGGGT 163 26 0 34.62 60.524 5.00 1.00 6.524 453 CATGTACACACTGAAGATGTTCCCT 100 25 0 44.00 61.548 6.00 4.00 6.548 454 ACTGAAGATGTTCCCTAAGAATGT 109 24 0 37.50 57.442 6.00 1.00 6.558 455 ACACTGAAGATGTTCCCTAAGAAT 107 24 0 37.50 57.442 6.00 2.00 6.558 456 CAAAAGCTAAGACATTGTGGGTGTA 167 25 0 40.00 61.563 5.00 2.00 6.563 457 TACAAAAGCTAAGACATTGTGGGTG 165 25 0 40.00 61.563 5.00 0.00 6.563 458 CCTTCCCTAATACAAAAGCTAAGA 155 24 0 37.50 57.427 4.00 1.00 6.573 459 CACACTGAACACGTTCCCTAAGAA 55 24 0 45.83 62.619 6.00 2.00 6.619 460 GTACACACTGAAGATGTTCCCTAA 103 24 0 41.67 57.376 6.00 1.00 6.624 461 ACATTGTGGGTGTACCACACACTT 178 24 0 45.83 62.627 8.00 6.00 6.627 462 GAAGACTTCCCTACATACAGCCAAG 14 25 0 48.00 61.639 7.00 4.00 6.639 463 ACACTGAAGATGTTCCCTAAGAATGT 107 26 0 38.46 60.648 6.00 1.00 6.648 464 ACACACTGAAGATGTTCCCTAAGAAT 105 26 0 38.46 60.648 6.00 2.00 6.648 465 GTACACACTGAAGATGTTCCCTAAG 103 25 0 44.00 58.344 6.00 3.00 6.656 466 GACCTTCCCTAATACAAAAGCTAA 153 24 0 37.50 57.300 4.00 1.00 6.700 467 AAGACCTTCCCTAATACAAAAGCTAA 151 26 0 34.62 59.264 4.00 1.00 6.736 468 AAAGCTAAGACATTGTGGGTGTACC 169 25 0 44.00 61.766 5.00 4.00 6.766 469 TCCCTAATACAAAAGCTAAGACATTG 158 26 0 34.62 59.231 4.00 3.00 6.769 470 CTAAGACATTGTGGGTGTACCACAC 173 25 0 48.00 61.794 6.00 6.00 6.794 471 TGTACACACTGAAGATGTTCCCTAAG 102 26 0 42.31 60.811 6.00 3.00 6.811 472 CCTTCCCTAATACAAAAGCTAAGAC 155 25 0 40.00 58.167 4.00 1.00 6.833 473 ACCTTCCCTAATACAAAAGCTAAGA 154 25 0 36.00 58.167 4.00 1.00 6.833 474 GACCTTCCCTAATACAAAAGCTAAG 153 25 0 40.00 58.167 4.00 3.00 6.833 475 AGACCTTCCCTAATACAAAAGCTAA 152 25 0 36.00 58.167 4.00 1.00 6.833 476 AAGACCTTCCCTAATACAAAAGCTA 151 25 0 36.00 58.167 4.00 2.00 6.833 477 CACTGAAGACTTCCCTACATACAG 10 24 0 45.83 57.151 7.00 2.00 6.849 478 GAATGCACTGAAGACCTTCCCTAA 141 24 0 45.83 62.910 6.00 1.00 6.910 479 GCACTGAAGACCTTCCCTAATACAA 145 25 0 44.00 61.945 6.00 0.00 6.945 480 CACACTCACTGAAGACTTCCCTACA 4 25 0 48.00 61.960 7.00 1.00 6.960 481 AAGACCTTCCCTAATACAAAAGCTAAG 151 27 0 37.04 59.997 4.00 3.00 7.003 482 CACTGAAGACCTTCCCTAATACAAAA 146 26 0 38.46 61.028 6.00 1.00 7.028 483 AGACCTTCCCTAATACAAAAGCTAAG 152 26 0 38.46 58.971 4.00 3.00 7.029 484 ACACGTTCCCTAAGAAATGTGGTTA 63 25 0 40.00 62.057 8.00 2.00 7.057 485 TCCCTAATACAAAAGCTAAGACATTGT 158 27 0 33.33 59.891 5.00 5.00 7.109 486 TACACACTGAACACGTTCCCTAAGA 53 25 0 44.00 62.121 6.00 1.00 7.121 487 ACCTTCCCTAATACAAAAGCTAAGAC 154 26 0 38.46 58.862 4.00 1.00 7.138 488 GTACCACACACTTCCTACAGCTGTG 189 25 0 52.00 62.233 8.00 6.00 7.233 489 TGTACCACACACTTCCTACAGCTGT 188 25 0 48.00 62.233 8.00 8.00 7.233 490 GTGTACCACACACTTCCTACAGCTG 187 25 0 52.00 62.233 6.00 6.00 7.233 491 GTTCCCTAAGAAATGTGGTTAAGGG 67 25 0 44.00 62.241 4.00 4.00 7.241 492 ACAAAAGCTAAGACATTGTGGGTGT 166 25 0 40.00 62.258 5.00 0.00 7.258 493 GATCACACTCACTGAAGACTTCCCT 1 25 0 48.00 62.262 7.00 5.00 7.262 494 TTCCCTAATACAAAAGCTAAGACATTG 157 27 0 33.33 60.279 4.00 3.00 7.279 495 ATCACACTCACTGAAGACTTCCCTAC 2 26 0 46.15 61.279 7.00 1.00 7.279 496 GACCTTCCCTAATACAAAAGCTAAGAC 153 27 0 40.74 60.283 4.00 1.00 7.283 497 CCCTAATACAAAAGCTAAGACATTG 159 25 0 36.00 57.709 4.00 3.00 7.291 498 CTGAAGATGTTCCCTAAGAATGTGG 110 25 0 44.00 62.299 6.00 0.00 7.299 499 ACACTCACTGAAGACTTCCCTACATAC 5 27 0 44.44 60.375 7.00 0.00 7.375 500 AGACCTTCCCTAATACAAAAGCTAAGA 152 27 0 37.04 60.382 4.00 1.00 7.382 501 GTGTACACACTGAACACGTTCCCTA 50 25 0 48.00 62.441 8.00 2.00 7.441 502 CACGTTCCCTAAGAAATGTGGTTAA 64 25 0 40.00 62.450 8.00 4.00 7.450 503 ACTCACTGAAGACTTCCCTACATACAG 7 27 0 44.44 60.484 7.00 2.00 7.484 504 CATGTACACACTGAAGATGTTCCCTA 100 26 0 42.31 61.505 6.00 2.00 7.505 505 CTTCCCTAATACAAAAGCTAAGACA 156 25 0 36.00 57.487 4.00 0.00 7.513 506 TAATACAAAAGCTAAGACATTGTGGGT 162 27 0 33.33 60.517 5.00 1.00 7.517 507 CCCTAATACAAAAGCTAAGACATTGT 159 26 0 34.62 58.432 5.00 5.00 7.568 508 CCTTCCCTAATACAAAAGCTAAGACAT 155 27 0 37.04 60.573 4.00 2.00 7.573 509 CTAATACAAAAGCTAAGACATTGTGGG 161 27 0 37.04 60.616 5.00 0.00 7.616 510 CCTAATACAAAAGCTAAGACATTGTGG 160 27 0 37.04 60.616 5.00 1.00 7.616 511 CCCTAATACAAAAGCTAAGACATTGTG 159 27 0 37.04 60.616 5.00 3.00 7.616 512 TACACACTGAAGATGTTCCCTAAGAAT 104 27 0 37.04 60.637 6.00 2.00 7.637 513 AGCTAAGACATTGTGGGTGTACCAC 171 25 0 48.00 62.652 5.00 4.00 7.652 514 GAAGACCTTCCCTAATACAAAAGCTAA 150 27 0 37.04 60.660 6.00 1.00 7.660 515 TCCCTAAGAAATGTGGTTAAGGGAC 69 25 0 44.00 62.702 6.00 2.00 7.702 516 ATACAAAAGCTAAGACATTGTGGGTG 164 26 0 38.46 61.720 5.00 0.00 7.720 517 TGAAGACCTTCCCTAATACAAAAGCT 149 26 0 38.46 61.823 6.00 4.00 7.823 518 CTGAAGACCTTCCCTAATACAAAAGC 148 26 0 42.31 61.823 6.00 2.00 7.823 519 ACTCACTGAAGACTTCCCTACATAC 7 25 0 44.00 57.154 7.00 0.00 7.846 520 GGTGTACCACACACTTCCTACAGCT 186 25 0 52.00 62.860 5.00 4.00 7.860 521 TGTACACACTGAACACGTTCCCTAA 51 25 0 44.00 62.865 6.00 1.00 7.865 522 TTCCCTAATACAAAAGCTAAGACATT 157 26 0 30.77 58.116 4.00 3.00 7.884 523 CTGAAGACTTCCCTACATACAGCCA 12 25 0 48.00 62.932 7.00 0.00 7.932 524 ACCTTCCCTAATACAAAAGCTAAGACA 154 27 0 37.04 60.995 4.00 0.00 7.995 525 ATGTACACACTGAAGATGTTCCCTAAG 101 27 0 40.74 61.005 6.00 3.00 8.005 526 AATGCACTGAAGACCTTCCCTAATAC 142 26 0 42.31 62.082 6.00 1.00 8.082 527 CTTCCCTAATACAAAAGCTAAGACATT 156 27 0 33.33 58.903 4.00 3.00 8.097 528 GTACACACTGAAGATGTTCCCTAAGAA 103 27 0 40.74 61.101 6.00 2.00 8.101 529 CACACTCACTGAAGACTTCCCTACAT 4 26 0 46.15 62.110 7.00 2.00 8.110 530 TCACTGAAGACTTCCCTACATACAGC 9 26 0 46.15 62.145 7.00 2.00 8.145 531 CTTCCCTAATACAAAAGCTAAGACAT 156 26 0 34.62 57.811 4.00 2.00 8.189 532 GATCACACTCACTGAAGACTTCCCTA 1 26 0 46.15 62.195 7.00 3.00 8.195 533 CAAAAGCTAAGACATTGTGGGTGTAC 167 26 0 42.31 62.195 5.00 4.00 8.195 534 ACAAAAGCTAAGACATTGTGGGTGTA 166 26 0 38.46 62.195 5.00 2.00 8.195 535 TACAAAAGCTAAGACATTGTGGGTGT 165 26 0 38.46 62.195 5.00 0.00 8.195 536 CTAATACAAAAGCTAAGACATTGTGG 161 26 0 34.62 57.766 5.00 1.00 8.234 537 CCTAATACAAAAGCTAAGACATTGTG 160 26 0 34.62 57.766 5.00 3.00 8.234 538 CACTGAAGATGTTCCCTAAGAATGTG 108 26 0 42.31 62.327 6.00 0.00 8.327 539 CACACTGAAGATGTTCCCTAAGAATG 106 26 0 42.31 62.327 6.00 0.00 8.327 540 CACTGAAGACCTTCCCTAATACAAAAG 146 27 0 40.74 61.738 6.00 3.00 8.738 541 GTACACACTGAACACGTTCCCTAAGA 52 26 0 46.15 62.761 6.00 1.00 8.761 542 AAAAGCTAAGACATTGTGGGTGTACC 168 26 0 42.31 62.764 5.00 4.00 8.764 543 TGAAGACCTTCCCTAATACAAAAGCTA 149 27 0 37.04 61.778 6.00 2.00 8.778 544 GTGTACCACACACTTCCTACAGCTGT 187 26 0 50.00 62.885 8.00 8.00 8.885 545 ACTGAAGATGTTCCCTAAGAATGTGG 109 26 0 42.31 62.911 6.00 0.00 8.911 546 GAATGCACTGAAGACCTTCCCTAATA 141 26 0 42.31 62.924 6.00 3.00 8.924 547 GCACTGAAGACCTTCCCTAATACAAA 145 26 0 42.31 62.934 6.00 0.00 8.934 548 CACTCACTGAAGACTTCCCTACATACA 6 27 0 44.44 62.050 7.00 0.00 9.050 549 CACACTCACTGAAGACTTCCCTACATA 4 27 0 44.44 62.050 7.00 2.00 9.050 550 TACAAAAGCTAAGACATTGTGGGTGTA 165 27 0 37.04 62.136 5.00 2.00 9.136 551 ATACAAAAGCTAAGACATTGTGGGTGT 164 27 0 37.04 62.320 5.00 0.00 9.320 552 TGTACACACTGAAGATGTTCCCTAAGA 102 27 0 40.74 62.355 6.00 1.00 9.355 553 ACTGAAGACCTTCCCTAATACAAAAGC 147 27 0 40.74 62.397 6.00 2.00 9.397 554 CTGAAGACCTTCCCTAATACAAAAGCT 148 27 0 40.74 62.483 6.00 4.00 9.483 555 CATGTACACACTGAAGATGTTCCCTAA 100 27 0 40.74 62.561 6.00 1.00 9.561 556 AATACAAAAGCTAAGACATTGTGGGTG 163 27 0 37.04 62.689 5.00 0.00 9.689 557 ACAAAAGCTAAGACATTGTGGGTGTAC 166 27 0 40.74 62.787 5.00 4.00 9.787 558 GATCACACTCACTGAAGACTTCCCTAC 1 27 0 48.15 62.829 7.00 1.00 9.829 559 CTCACTGAAGACTTCCCTACATACAGC 8 27 0 48.15 62.853 7.00 2.00 9.853 560 ACACTGAAGATGTTCCCTAAGAATGTG 107 27 0 40.74 62.934 6.00 0.00 9.934 561 CACACTGAAGATGTTCCCTAAGAATGT 106 27 0 40.74 62.934 6.00 1.00 9.934 562 ACACACTGAAGATGTTCCCTAAGAATG 105 27 0 40.74 62.934 6.00 0.00 9.934 primer3-2.3.6/test/primer1_list_last/SP01-03.txt.rev0000664000077300007730000001001112231156243020720 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 GATCCTGCTGAAGCAAATGG 175 20 0 50.00 60.744 6.00 1.00 0.744 1 ATCCTGCTGAAGCAAATGGA 174 20 0 45.00 60.744 6.00 1.00 0.744 2 CTGAAGCAAATGGACGTGG 168 19 0 52.63 60.247 4.00 0.00 1.247 3 TCCTGCTGAAGCAAATGGAC 173 20 0 50.00 61.329 6.00 1.00 1.329 4 CTGCTGAAGCAAATGGACGT 171 20 0 50.00 61.375 6.00 4.00 1.375 5 GTCACCAAATGCACCCAGAC 103 20 0 55.00 61.396 4.00 1.00 1.396 6 TGCTGAAGCAAATGGACGT 170 19 0 47.37 60.406 6.00 4.00 1.406 7 GCTGAAGCAAATGGACGTG 169 19 0 52.63 60.406 4.00 2.00 1.406 8 TCCTGCTGAAGCAAATGGA 173 19 0 47.37 60.487 6.00 1.00 1.487 9 GTCACCAAATGCACCCAGA 103 19 0 52.63 60.527 4.00 0.00 1.527 10 TCACCAAATGCACCCAGAC 102 19 0 52.63 60.527 4.00 1.00 1.527 11 CTGCTGAAGCAAATGGACG 171 19 0 52.63 60.546 6.00 2.00 1.546 12 CCTGCTGAAGCAAATGGAC 172 19 0 52.63 59.384 6.00 1.00 1.616 13 ACCAAATGCACCCAGACAC 100 19 0 52.63 59.383 4.00 0.00 1.617 14 AGCAAATGGACGTGGCAT 164 18 0 50.00 60.080 4.00 4.00 2.080 15 ATCCTGCTGAAGCAAATGG 174 19 0 47.37 58.811 6.00 1.00 2.189 16 GAAGCAAATGGACGTGGC 166 18 0 55.56 60.210 4.00 2.00 2.210 17 CTGCGGTGTCACCAAATG 110 18 0 55.56 59.652 7.00 3.00 2.348 18 GTGTCACCAAATGCACCCAG 105 20 0 55.00 62.415 5.00 1.00 2.415 19 CACCAAATGCACCCAGACAC 101 20 0 55.00 62.415 4.00 0.00 2.415 20 TCACCAAATGCACCCAGA 102 18 0 50.00 59.577 4.00 0.00 2.423 21 GTGTCACCAAATGCACCCA 105 19 0 52.63 61.452 5.00 0.00 2.452 22 AAGCAAATGGACGTGGCAT 165 19 0 47.37 61.465 4.00 4.00 2.465 23 CGGTGTCACCAAATGCAC 107 18 0 55.56 59.492 7.00 2.00 2.508 24 TGCTGAAGCAAATGGACG 170 18 0 50.00 59.490 6.00 2.00 2.510 25 ATCCTGCTGAAGCAAATGGAC 174 21 0 47.62 61.530 6.00 1.00 2.530 26 CCTGCGGTGTCACCAAAT 111 18 0 55.56 60.534 7.00 3.00 2.534 27 TGTCACCAAATGCACCCA 104 18 0 50.00 60.535 4.00 0.00 2.535 28 TGTCACCAAATGCACCCAG 104 19 0 52.63 61.589 4.00 1.00 2.589 29 CACCAAATGCACCCAGACA 101 19 0 52.63 61.589 4.00 0.00 2.589 30 TGAAGCAAATGGACGTGG 167 18 0 50.00 59.165 4.00 0.00 2.835 31 CAAATGGACGTGGCATCC 162 18 0 55.56 60.915 4.00 3.00 2.915 32 AGCAAATGGACGTGGCATC 164 19 0 52.63 62.050 4.00 2.00 3.050 33 GCAAATGGACGTGGCATC 163 18 0 55.56 61.080 4.00 2.00 3.080 34 AAGCAAATGGACGTGGCA 165 18 0 50.00 61.248 4.00 3.00 3.248 35 GGTGTCACCAAATGCACCC 106 19 0 57.89 62.254 7.00 1.00 3.254 36 GATCCTGCTGAAGCAAATGGA 175 21 0 47.62 62.559 6.00 1.00 3.559 37 TCCTGCTGAAGCAAATGG 173 18 0 50.00 58.427 6.00 1.00 3.573 38 CCTGCTGAAGCAAATGGA 172 18 0 50.00 58.427 6.00 0.00 3.573 39 GTCACCAAATGCACCCAG 103 18 0 55.56 58.389 4.00 1.00 3.611 40 CACCAAATGCACCCAGAC 101 18 0 55.56 58.389 4.00 1.00 3.611 41 ACCAAATGCACCCAGACA 100 18 0 50.00 58.389 4.00 0.00 3.611 42 CTCTGCCTGCGGTGTCAC 116 18 0 66.67 61.691 5.00 5.00 3.691 43 GGTGTCACCAAATGCACC 106 18 0 55.56 58.218 7.00 3.00 3.782 44 GTGTCACCAAATGCACCC 105 18 0 55.56 58.218 5.00 0.00 3.782 45 GCTGAAGCAAATGGACGT 169 18 0 50.00 57.302 4.00 4.00 4.698 46 TGCGGTGTCACCAAATGC 109 18 0 55.56 62.807 7.00 2.00 4.807 47 GCGGTGTCACCAAATGCA 108 18 0 55.56 62.807 7.00 4.00 4.807 48 AAATGGACGTGGCATCCG 161 18 0 55.56 62.891 4.00 2.00 4.891 primer3-2.3.6/test/primer1_list_last/included_test_w_middle.rev0000664000077300007730000006241112231156243023704 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 CACAACAGACACACACACACAA 187 22 0 45.45 59.121 1.00 0.00 2.879 1 CAACAGACACACACACACAACA 185 22 0 45.45 59.121 1.00 0.00 2.879 2 CACACACACAACACACAGTCAA 176 22 0 45.45 59.121 3.00 0.00 2.879 3 ACACAACAGACACACACACACAA 188 23 0 43.48 60.064 1.00 0.00 3.064 4 CACAACAGACACACACACACAAC 187 23 0 47.83 60.064 1.00 0.00 3.064 5 ACAACAGACACACACACACAACA 186 23 0 43.48 60.064 1.00 0.00 3.064 6 CAACAGACACACACACACAACAC 185 23 0 47.83 60.064 1.00 0.00 3.064 7 AACAGACACACACACACAACACA 184 23 0 43.48 60.064 1.00 0.00 3.064 8 ACACACACACAACACACAGTCAA 177 23 0 43.48 60.064 3.00 0.00 3.064 9 CACACACACAACACACAGTCAAG 176 23 0 47.83 60.208 3.00 0.00 3.208 10 CACACACAACACACAGTCAAGAA 174 23 0 43.48 59.744 3.00 0.00 3.256 11 AGACACACACACACAACACACAG 181 23 0 47.83 59.650 1.00 1.00 3.350 12 ACACAACAGACACACACACACA 188 22 0 45.45 58.514 1.00 0.00 3.486 13 ACAGACACACACACACAACACA 183 22 0 45.45 58.514 1.00 0.00 3.486 14 CAGACACACACACACAACACAC 182 22 0 50.00 58.514 1.00 0.00 3.486 15 AGACACACACACACAACACACA 181 22 0 45.45 58.514 1.00 0.00 3.486 16 GACACACACACACAACACACAG 180 22 0 50.00 58.514 1.00 1.00 3.486 17 CACACACACACAACACACAGTC 178 22 0 50.00 58.514 3.00 1.00 3.486 18 ACACACACACAACACACAGTCA 177 22 0 45.45 58.514 3.00 1.00 3.486 19 ACAGACACACACACACAACACAC 183 23 0 47.83 59.499 1.00 0.00 3.501 20 GACACACACACACAACACACAGT 180 23 0 47.83 59.499 3.00 3.00 3.501 21 ACACACACACACAACACACAGTC 179 23 0 47.83 59.499 3.00 1.00 3.501 22 CACAACAGACACACACACACA 187 21 0 47.62 57.434 1.00 0.00 3.566 23 CAGACACACACACACAACACA 182 21 0 47.62 57.434 1.00 0.00 3.566 24 CACACACACAACACACAGTCA 176 21 0 47.62 57.434 3.00 1.00 3.566 25 TGCACAATACCACACATGTTT 122 21 0 38.10 57.391 6.00 6.00 3.609 26 GACACACACACACAACACACA 180 21 0 47.62 57.244 1.00 0.00 3.756 27 CACACACAACACACAGTCAAGA 174 22 0 45.45 58.217 3.00 2.00 3.783 28 ACACACACAACACACAGTCAAGA 175 23 0 43.48 59.188 3.00 2.00 3.812 29 CACACAACACACAGTCAAGAAGC 172 23 0 47.83 60.832 3.00 2.00 3.832 30 CACAACACACAGTCAAGAAGCT 170 22 0 45.45 58.075 4.00 4.00 3.925 31 CACAGTCAAGAAGCTACATCCA 163 22 0 45.45 58.036 4.00 0.00 3.964 32 ACACAACACACAGTCAAGAAGCT 171 23 0 43.48 58.981 4.00 4.00 4.019 33 TACACAACAGACACACACACACAA 189 24 0 41.67 60.073 2.00 0.00 4.073 34 CACACAGTCAAGAAGCTACATCC 165 23 0 47.83 58.925 4.00 0.00 4.075 35 ACACAGTCAAGAAGCTACATCCA 164 23 0 43.48 58.925 4.00 0.00 4.075 36 TGCACAATACCACACATGTTTAA 122 23 0 34.78 58.925 6.00 4.00 4.075 37 ACACAACACACAGTCAAGAAGC 171 22 0 45.45 57.918 3.00 2.00 4.082 38 ATGCACAATACCACACATGTTT 123 22 0 36.36 57.811 6.00 6.00 4.189 39 ACACACAGTCAAGAAGCTACATCC 166 24 0 45.83 59.748 4.00 0.00 4.252 40 TGCACAATACCACACATGTTTAAC 122 24 0 37.50 59.733 6.00 5.00 4.267 41 GCACAATACCACACATGTTTAACA 121 24 0 37.50 59.733 7.00 7.00 4.267 42 TACACAACAGACACACACACACA 189 23 0 43.48 58.590 2.00 0.00 4.410 43 TGCACAATACCACACATGTTTA 122 22 0 36.36 57.504 6.00 4.00 4.496 44 GCTACATCCATACACAACACACA 151 23 0 43.48 58.499 2.00 0.00 4.501 45 AGCTACATCCATACACAACACACA 152 24 0 41.67 59.493 4.00 0.00 4.507 46 AGACACACACACACAACACACAGT 181 24 0 45.83 60.539 3.00 3.00 4.539 47 CACAGTCAAGAAGCTACATCCAT 163 23 0 43.48 58.408 4.00 2.00 4.592 48 ACACACACAACACACAGTCAAGAA 175 24 0 41.67 60.599 3.00 0.00 4.599 49 ACACACACACACAACACACAGT 179 22 0 45.45 57.352 3.00 3.00 4.648 50 CACAACACACATGTATATGCACAA 139 24 0 37.50 59.345 6.00 2.00 4.655 51 CAGACACACACACACAACACACA 182 23 0 47.83 61.701 1.00 0.00 4.701 52 CACACACACACAACACACAGTCA 178 23 0 47.83 61.701 3.00 1.00 4.701 53 CACACACAACACACAGTCAAGAAG 174 24 0 45.83 60.730 3.00 0.00 4.730 54 ACACAGTCAAGAAGCTACATCCAT 164 24 0 41.67 59.238 4.00 2.00 4.762 55 ATGCACAATACCACACATGTTTAA 123 24 0 33.33 59.233 6.00 4.00 4.767 56 CACAACACACAGTCAAGAAGCTA 170 23 0 43.48 58.160 4.00 2.00 4.840 57 CAACACACAGTCAAGAAGCTACA 168 23 0 43.48 58.160 4.00 2.00 4.840 58 GAAGCTACATCCATACACAACACA 154 24 0 41.67 59.091 4.00 0.00 4.909 59 ACACACACAACACACAGTCAAG 175 22 0 45.45 57.090 3.00 0.00 4.910 60 ACACAACAGACACACACACACAAC 188 24 0 45.83 60.929 1.00 0.00 4.929 61 ACAACAGACACACACACACAACAC 186 24 0 45.83 60.929 1.00 0.00 4.929 62 AACAGACACACACACACAACACAC 184 24 0 45.83 60.929 1.00 0.00 4.929 63 ACACAACACACAGTCAAGAAGCTA 171 24 0 41.67 59.030 4.00 2.00 4.970 64 CACAACACACAGTCAAGAAGCTAC 170 24 0 45.83 59.030 4.00 4.00 4.970 65 ACAACACACAGTCAAGAAGCTACA 169 24 0 41.67 59.030 4.00 2.00 4.970 66 AGAAGCTACATCCATACACAACACA 155 25 0 40.00 60.002 4.00 0.00 5.002 67 ATGCACAATACCACACATGTTTAAC 123 25 0 36.00 59.991 6.00 5.00 5.009 68 ATACACAACAGACACACACACACA 190 24 0 41.67 58.961 2.00 0.00 5.039 69 ACACACACACAACACACAGTCAAG 177 24 0 45.83 61.059 3.00 0.00 5.059 70 CACAACACACATGTATATGCACA 139 23 0 39.13 57.923 6.00 4.00 5.077 71 TATGCACAATACCACACATGTTT 124 23 0 34.78 57.903 6.00 6.00 5.097 72 ATGCACAATACCACACATGTTTA 123 23 0 34.78 57.903 6.00 4.00 5.097 73 GAAGCTACATCCATACACAACACAC 154 25 0 44.00 59.880 4.00 0.00 5.120 74 ACACAACACACATGTATATGCACAA 140 25 0 36.00 60.151 6.00 2.00 5.151 75 GCTACATCCATACACAACACACAT 151 24 0 41.67 58.842 2.00 2.00 5.158 76 ACACACAACACACAGTCAAGAAG 173 23 0 43.48 57.837 3.00 0.00 5.163 77 ACACAACACACAGTCAAGAAGCTAC 171 25 0 44.00 59.835 4.00 4.00 5.165 78 TCAAGAAGCTACATCCATACACAA 158 24 0 37.50 58.834 4.00 0.00 5.166 79 ACACAACACACATGTATATGCACA 140 24 0 37.50 58.828 6.00 4.00 5.172 80 AGCTACATCCATACACAACACACAT 152 25 0 40.00 59.772 4.00 2.00 5.228 81 CAACACACAGTCAAGAAGCTACATC 168 25 0 44.00 60.272 4.00 2.00 5.272 82 ATACACAACAGACACACACACACAA 190 25 0 40.00 60.344 2.00 0.00 5.344 83 ACATCCATACACAACACACATGT 148 23 0 39.13 57.646 6.00 6.00 5.354 84 CACAACACACATGTATATGCACAAT 139 25 0 36.00 59.637 6.00 2.00 5.363 85 GTCAAGAAGCTACATCCATACACAA 159 25 0 40.00 59.615 4.00 0.00 5.385 86 TCAAGAAGCTACATCCATACACAAC 158 25 0 40.00 59.615 4.00 0.00 5.385 87 CAAGAAGCTACATCCATACACAACA 157 25 0 40.00 60.458 4.00 0.00 5.458 88 CAACACACAGTCAAGAAGCTACAT 168 24 0 41.67 58.520 4.00 4.00 5.480 89 GACACACACACACAACACACAGTC 180 24 0 50.00 61.504 3.00 1.00 5.504 90 CACAGTCAAGAAGCTACATCCATA 163 24 0 41.67 58.476 4.00 2.00 5.524 91 CAGTCAAGAAGCTACATCCATACA 161 24 0 41.67 58.476 4.00 0.00 5.524 92 TCAAGAAGCTACATCCATACACA 158 23 0 39.13 57.467 4.00 0.00 5.533 93 GCACAATACCACACATGTTTAACAG 121 25 0 40.00 60.599 7.00 5.00 5.599 94 ACACACAACACACAGTCAAGAAGC 173 24 0 45.83 61.613 3.00 2.00 5.613 95 CACACAGTCAAGAAGCTACATCCA 165 24 0 45.83 61.619 4.00 0.00 5.619 96 GTCAAGAAGCTACATCCATACACA 159 24 0 41.67 58.339 4.00 0.00 5.661 97 ACAACACACAGTCAAGAAGCTACAT 169 25 0 40.00 59.333 4.00 4.00 5.667 98 CACACATGTATATGCACAATACCA 134 24 0 37.50 58.315 6.00 4.00 5.685 99 CACATGTATATGCACAATACCACA 132 24 0 37.50 58.315 6.00 2.00 5.685 100 CATGTATATGCACAATACCACACA 130 24 0 37.50 58.315 6.00 0.00 5.685 101 TGTATATGCACAATACCACACATG 128 24 0 37.50 58.315 6.00 4.00 5.685 102 ACACAGTCAAGAAGCTACATCCATA 164 25 0 40.00 59.274 4.00 2.00 5.726 103 CACAGTCAAGAAGCTACATCCATAC 163 25 0 44.00 59.274 4.00 0.00 5.726 104 ACAGTCAAGAAGCTACATCCATACA 162 25 0 40.00 59.274 4.00 0.00 5.726 105 CAGTCAAGAAGCTACATCCATACAC 161 25 0 44.00 59.274 4.00 0.00 5.726 106 AGTCAAGAAGCTACATCCATACACA 160 25 0 40.00 59.274 4.00 0.00 5.726 107 CACACAACACACAGTCAAGAAGCT 172 24 0 45.83 61.730 4.00 4.00 5.730 108 TATGCACAATACCACACATGTTTAA 124 25 0 32.00 59.268 6.00 4.00 5.732 109 ATATGCACAATACCACACATGTTT 125 24 0 33.33 58.262 6.00 6.00 5.738 110 AAGCTACATCCATACACAACACACA 153 25 0 40.00 60.742 4.00 0.00 5.742 111 AAGCTACATCCATACACAACACA 153 23 0 39.13 57.257 4.00 0.00 5.743 112 AAGCTACATCCATACACAACACAC 153 24 0 41.67 58.137 4.00 0.00 5.863 113 CCATACACAACACACATGTATATGC 144 25 0 40.00 59.136 6.00 4.00 5.864 114 ACACACATGTATATGCACAATACCA 135 25 0 36.00 59.136 6.00 4.00 5.864 115 CACACATGTATATGCACAATACCAC 134 25 0 40.00 59.136 6.00 2.00 5.864 116 ACACATGTATATGCACAATACCACA 133 25 0 36.00 59.136 6.00 2.00 5.864 117 CACATGTATATGCACAATACCACAC 132 25 0 40.00 59.136 6.00 0.00 5.864 118 ACATGTATATGCACAATACCACACA 131 25 0 36.00 59.136 6.00 0.00 5.864 119 TGTATATGCACAATACCACACATGT 128 25 0 36.00 59.136 6.00 6.00 5.864 120 TACACAACAGACACACACACACAAC 189 25 0 44.00 60.904 2.00 0.00 5.904 121 GCACAATACCACACATGTTTAAC 121 23 0 39.13 57.060 6.00 5.00 5.940 122 AACACACAGTCAAGAAGCTACATCC 167 25 0 44.00 60.962 4.00 0.00 5.962 123 CAAGAAGCTACATCCATACACAA 157 23 0 39.13 57.036 4.00 0.00 5.964 124 TATACACAACAGACACACACACACA 191 25 0 40.00 59.013 4.00 0.00 5.987 125 TATGCACAATACCACACATGTTTAAC 124 26 0 34.62 60.001 6.00 5.00 6.001 126 TATGCACAATACCACACATGTTTA 124 24 0 33.33 57.988 6.00 4.00 6.012 127 ACACAGTCAAGAAGCTACATCCATAC 164 26 0 42.31 60.018 4.00 0.00 6.018 128 ACAGTCAAGAAGCTACATCCATACAC 162 26 0 42.31 60.018 4.00 0.00 6.018 129 CACAATACCACACATGTTTAACAGA 120 25 0 36.00 58.912 7.00 3.00 6.088 130 CAAGAAGCTACATCCATACACAAC 157 24 0 41.67 57.904 4.00 0.00 6.096 131 AAGAAGCTACATCCATACACAACA 156 24 0 37.50 57.904 4.00 0.00 6.096 132 ACACACATGTATATGCACAATACCAC 135 26 0 38.46 59.900 6.00 2.00 6.100 133 ACACATGTATATGCACAATACCACAC 133 26 0 38.46 59.900 6.00 0.00 6.100 134 CTACATCCATACACAACACACATG 150 24 0 41.67 57.896 4.00 4.00 6.104 135 TACACAACACACATGTATATGCACA 141 25 0 36.00 58.882 6.00 4.00 6.118 136 CACACACACAACACACAGTCAAGA 176 24 0 45.83 62.141 3.00 2.00 6.141 137 TACACAACACACATGTATATGCACAA 141 26 0 34.62 60.156 6.00 2.00 6.156 138 CTACATCCATACACAACACACATGT 150 25 0 40.00 58.761 6.00 6.00 6.239 139 TACATCCATACACAACACACATGT 149 24 0 37.50 57.747 6.00 6.00 6.253 140 ACATCCATACACAACACACATGTA 148 24 0 37.50 57.747 6.00 4.00 6.253 141 AAGAAGCTACATCCATACACAACAC 156 25 0 40.00 58.712 4.00 0.00 6.288 142 CACAATACCACACATGTTTAACAGAC 120 26 0 38.46 59.680 7.00 1.00 6.320 143 CACAACACACATGTATATGCACAATA 139 26 0 34.62 59.659 6.00 4.00 6.341 144 TATACACAACAGACACACACACACAA 191 26 0 38.46 60.342 4.00 0.00 6.342 145 GTCAAGAAGCTACATCCATACACAAC 159 26 0 42.31 60.343 4.00 0.00 6.343 146 CAACACACATGTATATGCACAATACC 137 26 0 38.46 60.346 6.00 4.00 6.346 147 AACACACATGTATATGCACAATACCA 136 26 0 34.62 60.346 6.00 4.00 6.346 148 TGTATATGCACAATACCACACATGTT 128 26 0 34.62 60.346 6.00 4.00 6.346 149 CATGTATATGCACAATACCACACAT 130 25 0 36.00 58.650 6.00 2.00 6.350 150 ATGTATATGCACAATACCACACATG 129 25 0 36.00 58.650 6.00 4.00 6.350 151 AACACACAGTCAAGAAGCTACATC 167 24 0 41.67 57.626 4.00 2.00 6.374 152 ACACAACACACATGTATATGCACAAT 140 26 0 34.62 60.393 6.00 2.00 6.393 153 AGTCAAGAAGCTACATCCATACACAA 160 26 0 38.46 60.455 4.00 0.00 6.455 154 ATATGCACAATACCACACATGTTTAA 125 26 0 30.77 59.536 6.00 4.00 6.464 155 GCTACATCCATACACAACACACATG 151 25 0 44.00 61.472 4.00 4.00 6.472 156 ACACACACAACACACAGTCAAGAAG 175 25 0 44.00 61.508 3.00 0.00 6.508 157 ACAGACACACACACACAACACACA 183 24 0 45.83 62.520 1.00 0.00 6.520 158 ACACACACACACAACACACAGTCA 179 24 0 45.83 62.520 3.00 1.00 6.520 159 ACATGTATATGCACAATACCACACAT 131 26 0 34.62 59.420 6.00 2.00 6.580 160 ATGTATATGCACAATACCACACATGT 129 26 0 34.62 59.420 6.00 6.00 6.580 161 AGAAGCTACATCCATACACAACAC 155 24 0 41.67 57.400 4.00 0.00 6.600 162 CATACACAACACACATGTATATGCA 143 25 0 36.00 58.378 6.00 6.00 6.622 163 CAGACACACACACACAACACACAG 182 24 0 50.00 62.641 1.00 1.00 6.641 164 TATATGCACAATACCACACATGTTT 126 25 0 32.00 58.331 6.00 6.00 6.669 165 ATATGCACAATACCACACATGTTTA 125 25 0 32.00 58.331 6.00 4.00 6.669 166 ATATACACAACAGACACACACACACA 192 26 0 38.46 59.329 4.00 0.00 6.671 167 CACACAACACACAGTCAAGAAGCTA 172 25 0 44.00 61.678 4.00 2.00 6.678 168 CACAACACACAGTCAAGAAGCTACA 170 25 0 44.00 61.678 4.00 2.00 6.678 169 AGAAGCTACATCCATACACAACACAC 155 26 0 42.31 60.727 4.00 0.00 6.727 170 CATCCATACACAACACACATGTAT 147 24 0 37.50 57.241 6.00 6.00 6.759 171 CACACACACACAACATACACTCTAGA 242 26 0 42.31 59.211 6.00 6.00 6.789 172 CACACACACAACATACACTCTAGACA 240 26 0 42.31 59.211 6.00 2.00 6.789 173 CACACACAACATACACTCTAGACACA 238 26 0 42.31 59.211 6.00 0.00 6.789 174 CACACAGTCAAGAAGCTACATCCAT 165 25 0 44.00 61.791 4.00 2.00 6.791 175 CATACACAACACACATGTATATGCAC 143 26 0 38.46 59.190 6.00 4.00 6.810 176 ATACACAACACACATGTATATGCACA 142 26 0 34.62 59.190 6.00 4.00 6.810 177 TCCATACACAACACACATGTATATGC 145 26 0 38.46 60.811 6.00 4.00 6.811 178 CACAATACCACACATGTTTAACAG 120 24 0 37.50 57.140 7.00 5.00 6.860 179 ACATCCATACACAACACACATGTAT 148 25 0 36.00 58.124 6.00 6.00 6.876 180 ACACACACACACAACATACACTCT 243 24 0 41.67 57.096 2.00 1.00 6.904 181 GTATATGCACAATACCACACATGTTT 127 26 0 34.62 59.095 6.00 6.00 6.905 182 TCTACACACACACACAACATACACTC 246 26 0 42.31 59.077 2.00 0.00 6.923 183 AAGCTACATCCATACACAACACACAT 153 26 0 38.46 60.948 4.00 2.00 6.948 184 TATATGCACAATACCACACATGTT 126 24 0 33.33 57.002 6.00 4.00 6.998 185 TCTACACACACACACAACATACACTCT 246 27 0 40.74 60.001 2.00 1.00 7.001 186 ACACACACACACAACATACACTCTAGA 243 27 0 40.74 60.001 6.00 6.00 7.001 187 CACACACACACAACATACACTCTAGAC 242 27 0 44.44 60.001 6.00 4.00 7.001 188 ACACACACACAACATACACTCTAGACA 241 27 0 40.74 60.001 6.00 2.00 7.001 189 CACACACACAACATACACTCTAGACAC 240 27 0 44.44 60.001 6.00 0.00 7.001 190 ACACACACAACATACACTCTAGACACA 239 27 0 40.74 60.001 6.00 0.00 7.001 191 ACAACACACAGTCAAGAAGCTACATC 169 26 0 42.31 61.001 4.00 2.00 7.001 192 ATACACAACAGACACACACACACAAC 190 26 0 42.31 61.121 2.00 0.00 7.121 193 CACACACAACATACACTCTAGACACAG 238 27 0 44.44 60.122 6.00 1.00 7.122 194 CATGTATATGCACAATACCACACATG 130 26 0 38.46 61.147 6.00 4.00 7.147 195 AACACACATGTATATGCACAATACC 136 25 0 36.00 57.851 6.00 4.00 7.149 196 GTATATGCACAATACCACACATGTT 127 25 0 36.00 57.851 6.00 4.00 7.149 197 CAAGAAGCTACATCCATACACAACAC 157 26 0 42.31 61.157 4.00 0.00 7.157 198 AAGAAGCTACATCCATACACAACACA 156 26 0 38.46 61.157 4.00 0.00 7.157 199 TACATCCATACACAACACACATGTA 149 25 0 36.00 57.841 6.00 4.00 7.159 200 CTACATCCATACACAACACACATGTA 150 26 0 38.46 58.815 6.00 4.00 7.185 201 ATATGCACAATACCACACATGTTTAAC 125 27 0 33.33 60.227 6.00 5.00 7.227 202 CATCCATACACAACACACATGTATATG 147 27 0 37.04 60.230 6.00 6.00 7.230 203 AACACACGTGTATATACACAACAGACA 203 27 0 37.04 59.748 12.00 1.00 7.252 204 TGCACAATACCACACATGTTTAACA 122 25 0 36.00 62.264 7.00 7.00 7.264 205 ACACACAGTCAAGAAGCTACATCCA 166 25 0 44.00 62.322 4.00 0.00 7.322 206 CACACGTGTATATACACAACAGACA 201 25 0 40.00 57.664 12.00 1.00 7.336 207 CACGTGTATATACACAACAGACACA 199 25 0 40.00 57.664 12.00 1.00 7.336 208 CGTGTATATACACAACAGACACACA 197 25 0 40.00 57.664 12.00 0.00 7.336 209 ATACACAACACACATGTATATGCACAA 142 27 0 33.33 60.389 6.00 2.00 7.389 210 TACACAACACACATGTATATGCACAAT 141 27 0 33.33 60.389 6.00 2.00 7.389 211 ACACAACACACATGTATATGCACAATA 140 27 0 33.33 60.389 6.00 4.00 7.389 212 CACAACACACATGTATATGCACAATAC 139 27 0 37.04 60.389 6.00 6.00 7.389 213 AGACACACACACACAACACACAGTC 181 25 0 48.00 62.414 3.00 1.00 7.414 214 TATATGCACAATACCACACATGTTTAA 126 27 0 29.63 59.560 6.00 4.00 7.440 215 ACACACAACACACAGTCAAGAAGCT 173 25 0 44.00 62.443 4.00 4.00 7.443 216 GATCTACACACACACACAACATACA 248 25 0 40.00 57.548 4.00 0.00 7.452 217 ACACACGTGTATATACACAACAGACA 202 26 0 38.46 58.508 12.00 1.00 7.492 218 CACACGTGTATATACACAACAGACAC 201 26 0 42.31 58.508 12.00 3.00 7.492 219 ACACGTGTATATACACAACAGACACA 200 26 0 38.46 58.508 12.00 1.00 7.492 220 CACGTGTATATACACAACAGACACAC 199 26 0 42.31 58.508 12.00 1.00 7.492 221 ACGTGTATATACACAACAGACACACA 198 26 0 38.46 58.508 12.00 0.00 7.492 222 CGTGTATATACACAACAGACACACAC 197 26 0 42.31 58.508 12.00 0.00 7.492 223 ATGTATATGCACAATACCACACATGTT 129 27 0 33.33 60.562 6.00 4.00 7.562 224 GATCTACACACACACACAACATACAC 248 26 0 42.31 58.431 4.00 0.00 7.569 225 ATATACACAACAGACACACACACACAA 192 27 0 37.04 60.577 4.00 0.00 7.577 226 TATATGCACAATACCACACATGTTTA 126 26 0 30.77 58.395 6.00 4.00 7.605 227 GATCTACACACACACACAACATACACT 248 27 0 40.74 59.376 4.00 1.00 7.624 228 ATCTACACACACACACAACATACACTC 247 27 0 40.74 59.376 2.00 0.00 7.624 229 CACACACACACAACATACACTCTAG 242 25 0 44.00 57.370 4.00 4.00 7.630 230 TGTATATACACAACAGACACACACACA 195 27 0 37.04 59.363 10.00 0.00 7.637 231 TATATACACAACAGACACACACACACA 193 27 0 37.04 59.363 6.00 0.00 7.637 232 CATCCATACACAACACACATGTATA 147 25 0 36.00 57.352 6.00 4.00 7.648 233 TCCATACACAACACACATGTATATG 145 25 0 36.00 57.352 6.00 6.00 7.648 234 CCATACACAACACACATGTATATGCA 144 26 0 38.46 61.654 6.00 6.00 7.654 235 CACACATGTATATGCACAATACCACA 134 26 0 38.46 61.654 6.00 2.00 7.654 236 CACATGTATATGCACAATACCACACA 132 26 0 38.46 61.654 6.00 0.00 7.654 237 ACACACGTGTATATACACAACAGACAC 202 27 0 40.74 59.293 12.00 3.00 7.707 238 ACACGTGTATATACACAACAGACACAC 200 27 0 40.74 59.293 12.00 1.00 7.707 239 ACGTGTATATACACAACAGACACACAC 198 27 0 40.74 59.293 12.00 0.00 7.707 240 CACACAGTCAAGAAGCTACATCCATA 165 26 0 42.31 61.740 4.00 2.00 7.740 241 CACAGTCAAGAAGCTACATCCATACA 163 26 0 42.31 61.740 4.00 0.00 7.740 242 CAGTCAAGAAGCTACATCCATACACA 161 26 0 42.31 61.740 4.00 0.00 7.740 243 CTACACACACACACAACATACACTCT 245 26 0 42.31 58.251 2.00 1.00 7.749 244 ACACACACACACAACATACACTCTAG 243 26 0 42.31 58.251 4.00 4.00 7.749 245 TCTACACACACACACAACATACACT 246 25 0 40.00 57.217 2.00 1.00 7.783 246 CTACACACACACACAACATACACTC 245 25 0 44.00 57.217 2.00 0.00 7.783 247 TACACACACACACAACATACACTCT 244 25 0 40.00 57.217 2.00 1.00 7.783 248 ACACACACACACAACATACACTCTA 243 25 0 40.00 57.217 3.00 3.00 7.783 249 TACATCCATACACAACACACATGTAT 149 26 0 34.62 58.200 6.00 6.00 7.800 250 ACATCCATACACAACACACATGTATA 148 26 0 34.62 58.200 6.00 4.00 7.800 251 CACAACACACAGTCAAGAAGCTACAT 170 26 0 42.31 61.844 4.00 4.00 7.844 252 GAACACACGTGTATATACACAACAGA 204 26 0 38.46 58.139 12.00 4.00 7.861 253 GTATATGCACAATACCACACATGTTTA 127 27 0 33.33 59.133 6.00 4.00 7.867 254 CTACATCCATACACAACACACATGTAT 150 27 0 37.04 59.113 6.00 6.00 7.887 255 ACACACACACAACACACAGTCAAGA 177 25 0 44.00 62.889 3.00 2.00 7.889 256 ACAACACACATGTATATGCACAATA 138 25 0 32.00 57.073 6.00 4.00 7.927 257 CAACACACATGTATATGCACAATAC 137 25 0 36.00 57.073 6.00 6.00 7.927 258 AGAACACACGTGTATATACACAACAGA 205 27 0 37.04 59.049 12.00 4.00 7.951 259 ATCCATACACAACACACATGTATATGC 146 27 0 37.04 61.010 6.00 4.00 8.010 260 ACAACACACATGTATATGCACAATACC 138 27 0 37.04 61.034 6.00 4.00 8.034 261 AACACACATGTATATGCACAATACCAC 136 27 0 37.04 61.034 6.00 2.00 8.034 262 CACACGTGTATATACACAACAGACACA 201 27 0 40.74 61.042 12.00 1.00 8.042 263 CACGTGTATATACACAACAGACACACA 199 27 0 40.74 61.042 12.00 0.00 8.042 264 CGTGTATATACACAACAGACACACACA 197 27 0 40.74 61.042 12.00 0.00 8.042 265 TCAAGAAGCTACATCCATACACAACA 158 26 0 38.46 62.056 4.00 0.00 8.056 266 GAACACACGTGTATATACACAACAGAC 204 27 0 40.74 58.926 12.00 2.00 8.074 267 ACAACACACATGTATATGCACAATAC 138 26 0 34.62 57.913 6.00 6.00 8.087 268 TATACACAACAGACACACACACACAAC 191 27 0 40.74 61.092 4.00 0.00 8.092 269 AGTCAAGAAGCTACATCCATACACAAC 160 27 0 40.74 61.126 4.00 0.00 8.126 270 GCTACATCCATACACAACACACATGT 151 26 0 42.31 62.163 6.00 6.00 8.163 271 GCACAATACCACACATGTTTAACAGA 121 26 0 38.46 62.185 7.00 3.00 8.185 272 AGCTACATCCATACACAACACACATG 152 26 0 42.31 62.266 4.00 4.00 8.266 273 CATCCATACACAACACACATGTATAT 147 26 0 34.62 57.730 6.00 6.00 8.270 274 ATCCATACACAACACACATGTATATG 146 26 0 34.62 57.730 6.00 6.00 8.270 275 ACACACAACACACAGTCAAGAAGCTA 173 26 0 42.31 62.367 4.00 2.00 8.367 276 CACACAACACACAGTCAAGAAGCTAC 172 26 0 46.15 62.367 4.00 4.00 8.367 277 ACACAACACACAGTCAAGAAGCTACA 171 26 0 42.31 62.367 4.00 2.00 8.367 278 GAAGCTACATCCATACACAACACACA 154 26 0 42.31 62.368 4.00 0.00 8.368 279 ATCTACACACACACACAACATACACT 247 26 0 38.46 57.620 2.00 1.00 8.380 280 ATGCACAATACCACACATGTTTAACA 123 26 0 34.62 62.397 7.00 7.00 8.397 281 TGTATATGCACAATACCACACATGTTT 128 27 0 33.33 61.445 6.00 6.00 8.445 282 ACACACAGTCAAGAAGCTACATCCAT 166 26 0 42.31 62.454 4.00 2.00 8.454 283 ACATCCATACACAACACACATGTATAT 148 27 0 33.33 58.526 6.00 6.00 8.474 284 ACACACAACATACACTCTAGACACAG 237 26 0 42.31 57.508 6.00 1.00 8.492 285 AGAACACACGTGTATATACACAACAG 205 26 0 38.46 57.375 12.00 6.00 8.625 286 ACACACACACAACATACACTCTAGAC 241 26 0 42.31 57.364 6.00 4.00 8.636 287 ACACACACAACATACACTCTAGACAC 239 26 0 42.31 57.364 6.00 0.00 8.636 288 TACACACACACACAACATACACTCTA 244 26 0 38.46 57.330 3.00 3.00 8.670 289 CTACACACACACACAACATACACTCTA 245 27 0 40.74 58.323 3.00 3.00 8.677 290 TACACACACACACAACATACACTCTAG 244 27 0 40.74 58.323 4.00 4.00 8.677 291 CATACACAACACACATGTATATGCACA 143 27 0 37.04 61.688 6.00 4.00 8.688 292 TACATCCATACACAACACACATGTATA 149 27 0 33.33 58.271 6.00 4.00 8.729 293 AACACACGTGTATATACACAACAGAC 203 26 0 38.46 57.237 12.00 2.00 8.763 294 AAGAAGCTACATCCATACACAACACAC 156 27 0 40.74 61.811 4.00 0.00 8.811 295 CACATGTATATGCACAATACCACACAT 132 27 0 37.04 61.814 6.00 2.00 8.814 296 ACATGTATATGCACAATACCACACATG 131 27 0 37.04 61.814 6.00 4.00 8.814 297 CATGTATATGCACAATACCACACATGT 130 27 0 37.04 61.814 6.00 6.00 8.814 298 CACAATACCACACATGTTTAACAGACA 120 27 0 37.04 62.089 7.00 3.00 9.089 299 GCTACATCCATACACAACACACATGTA 151 27 0 40.74 62.100 6.00 4.00 9.100 300 CCATACACAACACACATGTATATGCAC 144 27 0 40.74 62.312 6.00 4.00 9.312 301 ACACACATGTATATGCACAATACCACA 135 27 0 37.04 62.312 6.00 2.00 9.312 302 CACACATGTATATGCACAATACCACAC 134 27 0 40.74 62.312 6.00 0.00 9.312 303 ACACATGTATATGCACAATACCACACA 133 27 0 37.04 62.312 6.00 0.00 9.312 304 TATGCACAATACCACACATGTTTAACA 124 27 0 33.33 62.329 7.00 7.00 9.329 305 ACACACAGTCAAGAAGCTACATCCATA 166 27 0 40.74 62.383 4.00 2.00 9.383 306 CACACAGTCAAGAAGCTACATCCATAC 165 27 0 44.44 62.383 4.00 0.00 9.383 307 ACACAGTCAAGAAGCTACATCCATACA 164 27 0 40.74 62.383 4.00 0.00 9.383 308 CACAGTCAAGAAGCTACATCCATACAC 163 27 0 44.44 62.383 4.00 0.00 9.383 309 ACAGTCAAGAAGCTACATCCATACACA 162 27 0 40.74 62.383 4.00 0.00 9.383 310 GTGTATATACACAACAGACACACACAC 196 27 0 40.74 57.575 12.00 0.00 9.425 311 GTATATACACAACAGACACACACACAC 194 27 0 40.74 57.575 8.00 0.00 9.425 312 GAAGCTACATCCATACACAACACACAT 154 27 0 40.74 62.494 4.00 2.00 9.494 313 ACACAACACACAGTCAAGAAGCTACAT 171 27 0 40.74 62.495 4.00 4.00 9.495 314 TAGAACACACGTGTATATACACAACAG 206 27 0 37.04 57.472 12.00 6.00 9.528 315 GTCAAGAAGCTACATCCATACACAACA 159 27 0 40.74 62.685 4.00 0.00 9.685 316 TCAAGAAGCTACATCCATACACAACAC 158 27 0 40.74 62.685 4.00 0.00 9.685 317 CAACACACATGTATATGCACAATACCA 137 27 0 37.04 62.716 6.00 4.00 9.716 318 CAGTCAAGAAGCTACATCCATACACAA 161 27 0 40.74 62.778 4.00 0.00 9.778 319 GCACAATACCACACATGTTTAACAGAC 121 27 0 40.74 62.806 7.00 1.00 9.806 320 AGCTACATCCATACACAACACACATGT 152 27 0 40.74 62.902 6.00 6.00 9.902 primer3-2.3.6/test/primer1_list_last/targets_test.rev0000664000077300007730000000041112231156243021712 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 CAGTGTGTACATGGCGGC 161 18 0 61.11 59.685 6.00 3.00 2.315 primer3-2.3.6/test/primer1_list_last/long_seq.rev0000664000077300007730000021725112231156243021025 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 agctagctagcatcgatcgc 392 20 0 55.00 59.880 10.00 6.00 0.120 1 cccagctagctagcatcgtc 677 20 0 60.00 60.140 10.00 4.00 0.140 2 catcgatcgatgtagcatcg 336 20 0 50.00 60.205 12.00 8.00 0.205 3 gtagcatgcatgcatcgatc 465 20 0 50.00 60.222 10.00 6.00 0.222 4 agcatcgcctagctagcatc 428 20 0 55.00 59.742 8.00 4.00 0.258 5 gtcagctagctagatcgccg 601 20 0 60.00 60.278 8.00 6.00 0.278 6 ctagctagatcgccgctacg 596 20 0 60.00 60.285 8.00 2.00 0.285 7 cgatgcgctagctagtagcc 695 20 0 60.00 60.293 8.00 6.00 0.293 8 gctagctagcatcgcctagc 435 20 0 60.00 59.528 10.00 6.00 0.472 9 agctagctagatcgccgcta 598 20 0 55.00 59.523 8.00 4.00 0.477 10 ctagctagccgcacatcgat 105 20 0 55.00 60.531 8.00 6.00 0.531 11 gctagctagatcgccgctac 597 20 0 60.00 59.393 8.00 2.00 0.607 12 tcgcctagctagcatcgatc 424 20 0 55.00 60.615 8.00 4.00 0.615 13 gcctagctagcatcgatcga 422 20 0 55.00 60.615 8.00 8.00 0.615 14 tcgcgtatcagtcagctagc 487 20 0 55.00 59.351 6.00 6.00 0.649 15 ccagctagctagcatcgtca 676 20 0 55.00 59.347 10.00 1.00 0.653 16 agtagcatgcatgcatcgat 466 20 0 45.00 59.285 10.00 8.00 0.715 17 gatgcgctagctagtagccc 694 20 0 60.00 59.256 8.00 8.00 0.744 18 gcatcgatcgatgtagcatc 337 20 0 50.00 59.213 12.00 6.00 0.787 19 tgcgctagctagtagcccag 692 20 0 60.00 60.821 10.00 6.00 0.821 20 atcgcctagctagcatcgat 425 20 0 50.00 59.074 8.00 6.00 0.926 21 cgctagctagtagcccagct 690 20 0 60.00 59.068 8.00 5.00 0.932 22 tagtagcatgcatgcatcga 467 20 0 45.00 58.989 10.00 8.00 1.011 23 ctagtagcatgcatgcatcga 468 21 0 47.62 60.014 10.00 8.00 1.014 24 gcatcgatcgatcagctagc 413 20 0 55.00 61.025 10.00 6.00 1.025 25 gctagcatcgatcgatcagtc 136 21 0 52.38 59.961 10.00 6.00 1.039 26 agcatcgatcgatcagtcag 133 20 0 50.00 58.936 10.00 2.00 1.064 27 catcgatcgatcagtcagct 131 20 0 50.00 58.936 10.00 4.00 1.064 28 tagctagctagcatcgatcgc 393 21 0 52.38 59.894 10.00 6.00 1.106 29 gctagctagcatcgatgatcg 568 21 0 52.38 60.117 10.00 8.00 1.117 30 tagctagatcgccgctacga 595 20 0 55.00 61.150 6.00 4.00 1.150 31 ctagctagcatcgatcgcg 390 19 0 57.89 59.848 8.00 4.00 1.152 32 cgcgcagagatgatagtagc 375 20 0 55.00 58.801 4.00 3.00 1.199 33 cgctacgatcgatcagctag 583 20 0 55.00 58.799 8.00 8.00 1.201 34 cgcgcagagatgatagtagct 375 21 0 52.38 59.789 4.00 4.00 1.211 35 agcatcgatcgatgtagcatc 338 21 0 47.62 60.222 12.00 6.00 1.222 36 tagcatcgcctagctagcatc 429 21 0 52.38 59.761 8.00 4.00 1.239 37 cgatcgatcagctagtcagct 615 21 0 52.38 59.755 8.00 5.00 1.245 38 cgatcgatcagtcagctagct 128 21 0 52.38 59.755 8.00 8.00 1.245 39 ctagctagccgcacatcga 105 19 0 57.89 60.272 8.00 4.00 1.272 40 cgatcgatcagctagtcagc 615 20 0 55.00 58.726 8.00 3.00 1.274 41 cgatcgatcagtcagctagc 128 20 0 55.00 58.726 8.00 6.00 1.274 42 catcgcctagctagcatcg 426 19 0 57.89 59.703 8.00 4.00 1.297 43 agctagctagatcgccgctac 598 21 0 57.14 60.297 8.00 2.00 1.297 44 ccagctagctagcatcgtcag 676 21 0 57.14 60.319 10.00 2.00 1.319 45 tcgcgtatcagtcagctagct 487 21 0 52.38 60.328 8.00 8.00 1.328 46 agctagcatcgcgtatcagtc 495 21 0 52.38 59.657 6.00 1.00 1.343 47 atcgcgtatcagtcagctagc 488 21 0 52.38 59.657 6.00 6.00 1.343 48 gctagcatcgcgtatcagtc 494 20 0 55.00 58.655 6.00 1.00 1.345 49 acgatcgatcagctagtcagc 616 21 0 52.38 59.618 8.00 3.00 1.382 50 agctagatcgccgctacgat 594 20 0 55.00 61.387 6.00 6.00 1.387 51 ctagcatcgcctagctagca 430 20 0 55.00 58.609 8.00 4.00 1.391 52 ccgctacgatcgatcagcta 584 20 0 55.00 61.420 8.00 6.00 1.420 53 agctagctagcatcgcctagc 436 21 0 57.14 60.424 10.00 6.00 1.424 54 gctagctagcatcgcctagct 435 21 0 57.14 60.424 10.00 8.00 1.424 55 tagctagccgcacatcgatc 104 20 0 55.00 61.432 6.00 4.00 1.432 56 tagctagccgcacatcgat 104 19 0 52.63 59.563 6.00 6.00 1.437 57 cagctagctagcatcgtcagc 675 21 0 57.14 60.464 10.00 2.00 1.464 58 gctagatcgccgctacgat 593 19 0 57.89 60.493 6.00 6.00 1.493 59 agctagctagatcgccgct 598 19 0 57.89 59.495 8.00 3.00 1.505 60 agcatcgcgtatcagtcagc 491 20 0 55.00 61.520 4.00 2.00 1.520 61 gcatcgcgtatcagtcagct 490 20 0 55.00 61.520 4.00 4.00 1.520 62 tagctagctagccgcacatc 108 20 0 55.00 58.466 10.00 0.00 1.534 63 cgcgtatcagtcagctagct 486 20 0 55.00 58.460 8.00 8.00 1.540 64 gcatcgcgtatcagtcagc 490 19 0 57.89 60.553 4.00 2.00 1.553 65 catcgcctagctagcatcga 426 20 0 55.00 61.554 8.00 4.00 1.554 66 ctagctagctagccgcacatc 109 21 0 57.14 59.442 12.00 0.00 1.558 67 tcagctagctagatcgccg 600 19 0 57.89 59.435 8.00 6.00 1.565 68 agcatcgatgatgatcagct 534 20 0 45.00 58.361 8.00 6.00 1.639 69 tagctagatcgccgctacg 595 19 0 57.89 59.339 6.00 2.00 1.661 70 tcgatcgatcagtcagctagc 129 21 0 52.38 60.661 8.00 6.00 1.661 71 tcgcgcagagatgatagtagc 376 21 0 52.38 60.662 4.00 3.00 1.662 72 gctagctagcatcgcgtatca 498 21 0 52.38 60.662 10.00 1.00 1.662 73 gcgctagctagtagcccagc 691 20 0 65.00 61.663 8.00 7.00 1.663 74 tagtagcatgcatgcatcgat 467 21 0 42.86 59.324 10.00 8.00 1.676 75 tagcatgcatgcatcgatc 464 19 0 47.37 59.309 10.00 7.00 1.691 76 agcatcgatcgatgtagcat 338 20 0 45.00 58.299 12.00 4.00 1.701 77 agcatcgatgatcagctagct 210 21 0 47.62 59.216 8.00 8.00 1.784 78 cgatcgatcagctagctagca 578 21 0 52.38 60.787 10.00 8.00 1.787 79 cgatcagctagctagcatcga 574 21 0 52.38 60.787 10.00 8.00 1.787 80 agctagcatcgatcgatcag 418 20 0 50.00 58.173 10.00 8.00 1.827 81 ctagcatcgatcgatcagct 416 20 0 50.00 58.173 10.00 8.00 1.827 82 agcatcgatcgatcagctag 414 20 0 50.00 58.173 10.00 6.00 1.827 83 catcgatcgatcagctagct 412 20 0 50.00 58.173 10.00 8.00 1.827 84 agctagcatcgatcgatcag 137 20 0 50.00 58.173 10.00 8.00 1.827 85 agcatcgatgatcagctagc 210 20 0 50.00 58.173 8.00 6.00 1.827 86 gcatcgatgatcagctagct 209 20 0 50.00 58.173 8.00 8.00 1.827 87 atcgcctagctagcatcgatc 425 21 0 52.38 60.845 8.00 4.00 1.845 88 cgctagctagtagcccagcta 690 21 0 57.14 59.110 8.00 6.00 1.890 89 gcatcgatcgatcagtcagc 132 20 0 55.00 61.922 10.00 3.00 1.922 90 ctagtagcatgcatgcatcg 468 20 0 50.00 58.074 10.00 8.00 1.926 91 agctagcatcgatcgatcagt 137 21 0 47.62 59.073 10.00 6.00 1.927 92 gcccagctagctagcatcgt 678 20 0 60.00 61.940 10.00 6.00 1.940 93 cctagctagcatcgatcgatc 421 21 0 52.38 59.060 10.00 8.00 1.940 94 cgatcgcgcagagatgatag 379 20 0 55.00 61.980 6.00 1.00 1.980 95 gatcgcgcagagatgatagt 378 20 0 50.00 58.018 4.00 1.00 1.982 96 gctagcatcgatcgatcagt 136 20 0 50.00 58.018 10.00 6.00 1.982 97 ctagcatcgatcgatcagtcag 135 22 0 50.00 60.000 10.00 6.00 2.000 98 catcgatcgatcagtcagctag 131 22 0 50.00 60.000 10.00 4.00 2.000 99 cgctagctagtagcccagctag 690 22 0 59.09 59.992 8.00 8.00 2.008 100 ctagcatcgatcgatcagtca 135 21 0 47.62 58.990 10.00 5.00 2.010 101 tagcatcgatcgatcagtcag 134 21 0 47.62 58.990 10.00 2.00 2.010 102 catcgatcgatcagtcagcta 131 21 0 47.62 58.990 10.00 2.00 2.010 103 tagcatcgatcgatgtagca 339 20 0 45.00 57.987 12.00 7.00 2.013 104 atgcgctagctagtagcccag 693 21 0 57.14 61.035 10.00 8.00 2.035 105 cgatcgcgcagagatgata 379 19 0 52.63 61.047 6.00 2.00 2.047 106 tagcatcgcctagctagcat 429 20 0 50.00 57.952 8.00 6.00 2.048 107 gctagctagcatcgcgtatc 498 20 0 55.00 57.951 10.00 2.00 2.049 108 agcccagctagctagcatcg 679 20 0 60.00 62.053 10.00 8.00 2.053 109 agctagctagcatcgcgtatc 499 21 0 52.38 58.940 10.00 2.00 2.060 110 ctagcatcgcctagctagcat 430 21 0 52.38 58.937 8.00 6.00 2.063 111 tcagctagctagcatcgatga 571 21 0 47.62 58.934 10.00 8.00 2.066 112 agtagcatgcatgcatcga 466 19 0 47.37 58.933 10.00 8.00 2.067 113 cgatgtagcatcgatcagtagc 329 22 0 50.00 59.895 8.00 2.00 2.105 114 gctagctagcatcgatcgc 391 19 0 57.89 58.889 10.00 6.00 2.111 115 agctagctacgatcgatcagc 624 21 0 52.38 58.876 8.00 6.00 2.124 116 gctagctacgatcgatcagct 623 21 0 52.38 58.876 8.00 8.00 2.124 117 gctacgatcgatcagctagct 582 21 0 52.38 58.876 10.00 8.00 2.124 118 tagcatcgatcgatcagtca 134 20 0 45.00 57.875 10.00 4.00 2.125 119 gctagctacgatcgatcagc 623 20 0 55.00 57.846 8.00 6.00 2.154 120 agtcagctagctagatcgccg 602 21 0 57.14 61.177 8.00 6.00 2.177 121 cagctagctagatcgccgct 599 20 0 60.00 62.179 8.00 3.00 2.179 122 gcccagctagctagcatcg 678 19 0 63.16 61.184 10.00 8.00 2.184 123 agctagatcgccgctacga 594 19 0 57.89 61.184 4.00 4.00 2.184 124 agctagctacgatcgatcagct 624 22 0 50.00 59.816 8.00 8.00 2.184 125 agtagcatgcatgcatcgatc 466 21 0 47.62 61.188 10.00 6.00 2.188 126 cgcgcagagatgatagtagcta 375 22 0 50.00 59.806 6.00 6.00 2.194 127 agctagcatcgcgtatcagt 495 20 0 50.00 57.781 6.00 1.00 2.219 128 tgcatcgatcgatgatagtagc 455 22 0 45.45 60.225 12.00 4.00 2.225 129 tagcatcgatcgatgtagcatc 339 22 0 45.45 60.225 12.00 6.00 2.225 130 cgatcgatcagctagtcagcta 615 22 0 50.00 59.775 8.00 3.00 2.225 131 cgatcgatcagtcagctagcta 128 22 0 50.00 59.775 8.00 6.00 2.225 132 gctagatcgccgctacga 593 18 0 61.11 60.228 4.00 4.00 2.228 133 ctagctagctagcatcgcgt 501 20 0 55.00 57.771 12.00 4.00 2.229 134 catgatgatcgatcagtcagct 285 22 0 45.45 60.249 12.00 7.00 2.249 135 gcatcgcctagctagcatc 427 19 0 57.89 58.740 8.00 4.00 2.260 136 atcgcctagctagcatcga 425 19 0 52.63 58.738 8.00 5.00 2.262 137 tcgcctagctagcatcgat 424 19 0 52.63 58.738 8.00 6.00 2.262 138 cgcctagctagcatcgatc 423 19 0 57.89 58.738 8.00 4.00 2.262 139 gcctagctagcatcgatcg 422 19 0 57.89 58.738 8.00 6.00 2.262 140 gctagatcgccgctacgatc 593 20 0 60.00 62.271 8.00 8.00 2.271 141 ctagtagcatgcatgcatcgat 468 22 0 45.45 60.280 10.00 8.00 2.280 142 ctagcatcgcgtatcagtcag 493 21 0 52.38 58.715 4.00 1.00 2.285 143 catcgcgtatcagtcagctag 489 21 0 52.38 58.715 4.00 4.00 2.285 144 agctagctagcatcgtcagct 674 21 0 52.38 58.692 10.00 4.00 2.308 145 cagctagctagatcgccgc 599 19 0 63.16 61.320 8.00 3.00 2.320 146 tagctagcatcgcgtatcagtc 496 22 0 50.00 59.680 6.00 1.00 2.320 147 tcgcgtatcagtcagctagcta 487 22 0 50.00 60.326 8.00 6.00 2.326 148 agctagctagcatcgtcagc 674 20 0 55.00 57.659 10.00 2.00 2.341 149 gctagctagcatcgtcagct 673 20 0 55.00 57.659 10.00 4.00 2.341 150 agctagcatcgtcagctagc 670 20 0 55.00 57.659 7.00 7.00 2.341 151 gctagcatcgtcagctagct 669 20 0 55.00 57.659 8.00 8.00 2.341 152 tagctagatcgccgctacgat 595 21 0 52.38 61.348 6.00 6.00 2.348 153 gctacgatcgatcagctagtca 619 22 0 50.00 59.643 8.00 2.00 2.357 154 tacgatcgatcagctagtcagc 617 22 0 50.00 59.643 8.00 3.00 2.357 155 ctagcatcgcgtatcagtca 493 20 0 50.00 57.632 4.00 1.00 2.368 156 tagcatcgcgtatcagtcag 492 20 0 50.00 57.632 4.00 1.00 2.368 157 catcgcgtatcagtcagcta 489 20 0 50.00 57.632 4.00 3.00 2.368 158 ctagctagctagccgcacat 109 20 0 55.00 57.626 12.00 2.00 2.374 159 ctagatcgccgctacgatc 592 19 0 57.89 58.588 8.00 8.00 2.412 160 tagctagctagcatcgcctagc 437 22 0 54.55 60.419 10.00 6.00 2.419 161 catgcatcgatcgatgatagt 457 21 0 42.86 58.563 12.00 8.00 2.437 162 gctagctagatcgccgcta 597 19 0 57.89 58.547 8.00 4.00 2.453 163 tagcccagctagctagcatc 680 20 0 55.00 57.541 10.00 6.00 2.459 164 ccgctacgatcgatcagct 584 19 0 57.89 61.465 8.00 4.00 2.465 165 agctagccgcacatcgat 103 18 0 55.56 59.533 6.00 6.00 2.467 166 cgcgtatcagtcagctagcta 486 21 0 52.38 58.528 8.00 6.00 2.472 167 gctagcatcgcgtatcagtca 494 21 0 52.38 61.473 6.00 1.00 2.473 168 tagcatcgcgtatcagtcagc 492 21 0 52.38 61.473 4.00 2.00 2.473 169 gcatcgcgtatcagtcagcta 490 21 0 52.38 61.473 4.00 3.00 2.473 170 agctagccgcacatcgatc 103 19 0 57.89 61.479 6.00 4.00 2.479 171 gccgctacgatcgatcag 585 18 0 61.11 60.490 8.00 6.00 2.490 172 ccgctacgatcgatcagc 584 18 0 61.11 60.490 8.00 6.00 2.490 173 gctagccgcacatcgatc 102 18 0 61.11 60.500 6.00 4.00 2.500 174 cgcgtatcagtcagctagctag 486 22 0 54.55 59.491 8.00 8.00 2.509 175 agctagcatcgatgatgatcag 538 22 0 45.45 59.462 8.00 6.00 2.538 176 ctagcatcgatgatgatcagct 536 22 0 45.45 59.462 8.00 6.00 2.538 177 agcatcgatgatgatcagctag 534 22 0 45.45 59.462 8.00 5.00 2.538 178 catcgatgatgatcagctagct 532 22 0 45.45 59.462 8.00 8.00 2.538 179 agctagcatgatgatcgatcag 291 22 0 45.45 59.462 10.00 8.00 2.538 180 agctagcatcgatgatgatca 538 21 0 42.86 58.438 8.00 7.00 2.562 181 gctagcatcgatgatgatcag 537 21 0 47.62 58.438 8.00 6.00 2.562 182 ctagcatcgatgatgatcagc 536 21 0 47.62 58.438 8.00 7.00 2.562 183 tagcatcgatgatgatcagct 535 21 0 42.86 58.438 8.00 6.00 2.562 184 agcatcgatgatgatcagcta 534 21 0 42.86 58.438 8.00 7.00 2.562 185 gcatcgatgatgatcagctag 533 21 0 47.62 58.438 8.00 5.00 2.562 186 catcgatgatgatcagctagc 532 21 0 47.62 58.438 8.00 6.00 2.562 187 gctagcatgatgatcgatcag 290 21 0 47.62 58.438 10.00 8.00 2.562 188 acgatcgatcagctagtcagct 616 22 0 50.00 60.566 8.00 5.00 2.566 189 atcgcgtatcagtcagctagct 488 22 0 50.00 60.570 8.00 8.00 2.570 190 gtagcccagctagctagcatc 681 21 0 57.14 58.416 10.00 6.00 2.584 191 agctagctagccgcacatc 107 19 0 57.89 58.394 10.00 0.00 2.606 192 tagcatcgatcgatgtagcat 339 21 0 42.86 58.376 12.00 4.00 2.624 193 ctagcatcgcctagctagcatc 430 22 0 54.55 60.642 8.00 4.00 2.642 194 agtagcccagctagctagcatc 682 22 0 54.55 59.355 10.00 6.00 2.645 195 gctagcatgatgatcgatcagt 290 22 0 45.45 59.325 10.00 8.00 2.675 196 gctagcatcgatgatgatca 537 20 0 45.00 57.307 8.00 7.00 2.693 197 tagcatcgatgatgatcagc 535 20 0 45.00 57.307 8.00 7.00 2.693 198 gcatcgatgatgatcagcta 533 20 0 45.00 57.307 8.00 7.00 2.693 199 agctagatcgccgctacg 594 18 0 61.11 59.301 4.00 2.00 2.699 200 gctagctagtagcatgcatgca 473 22 0 50.00 60.706 10.00 8.00 2.706 201 agcatgcatgcatcgatc 463 18 0 50.00 59.265 10.00 4.00 2.735 202 catcgcctagctagcatcgat 426 21 0 52.38 61.735 8.00 6.00 2.735 203 gatcagctagctagcatcgatg 573 22 0 50.00 59.256 10.00 8.00 2.744 204 atcagctagctagcatcgatga 572 22 0 45.45 59.256 10.00 8.00 2.744 205 tcagctagctagcatcgatgat 571 22 0 45.45 59.256 10.00 8.00 2.744 206 cagctagctagcatcgatgatc 570 22 0 50.00 59.256 10.00 6.00 2.744 207 tagcatcgatgatcagctagct 211 22 0 45.45 59.256 8.00 8.00 2.744 208 agcatcgatgatcagctagcta 210 22 0 45.45 59.256 8.00 6.00 2.744 209 gcatcgatgatcagctagctag 209 22 0 50.00 59.256 8.00 8.00 2.744 210 tagcatcgatgatcagctagc 211 21 0 47.62 58.255 8.00 6.00 2.745 211 gcatcgatgatcagctagcta 209 21 0 47.62 58.255 8.00 6.00 2.745 212 ctagctagcatcgatcgatca 420 21 0 47.62 58.254 10.00 6.00 2.746 213 tagctagcatcgatcgatcag 419 21 0 47.62 58.254 10.00 8.00 2.746 214 tagcatcgatcgatcagctag 415 21 0 47.62 58.254 10.00 7.00 2.746 215 catcgatcgatcagctagcta 412 21 0 47.62 58.254 10.00 6.00 2.746 216 ctagctagcatcgatcgatca 139 21 0 47.62 58.254 10.00 6.00 2.746 217 tagctagcatcgatcgatcag 138 21 0 47.62 58.254 10.00 8.00 2.746 218 ctagctagcatcgatcgatcag 420 22 0 50.00 59.252 10.00 8.00 2.748 219 catcgatcgatcagctagctag 412 22 0 50.00 59.252 10.00 8.00 2.748 220 ctagctagcatcgatcgatcag 139 22 0 50.00 59.252 10.00 8.00 2.748 221 cccagctagctagcatcgt 677 19 0 57.89 58.242 10.00 6.00 2.758 222 ctagctagctagcatcgatcgc 394 22 0 54.55 60.766 12.00 6.00 2.766 223 tagctagccgcacatcga 104 18 0 55.56 59.232 6.00 4.00 2.768 224 agtagcccagctagctagca 682 20 0 55.00 57.212 10.00 8.00 2.788 225 agctagtagcatgcatgcatc 470 21 0 47.62 58.187 10.00 8.00 2.813 226 gtagcatgcatgcatcgat 465 19 0 47.37 58.187 10.00 8.00 2.813 227 gctagcatactagcatcgatcg 349 22 0 50.00 59.173 10.00 6.00 2.827 228 tagctagcatcgatcgatca 419 20 0 45.00 57.158 10.00 6.00 2.842 229 tagctagcatcgatcgatca 138 20 0 45.00 57.158 10.00 6.00 2.842 230 gctagtagcatgcatgcatcg 469 21 0 52.38 61.851 10.00 8.00 2.851 231 agctagctagcatcgcgtat 499 20 0 50.00 57.129 10.00 4.00 2.871 232 gctagtagcatcgatgatcagc 216 22 0 50.00 59.121 8.00 8.00 2.879 233 gtagcatcgatgatcagctagc 212 22 0 50.00 59.121 8.00 6.00 2.879 234 gatcgcgcagagatgatagtag 378 22 0 50.00 59.118 4.00 1.00 2.882 235 actagctagcatcgatcgatca 140 22 0 45.45 59.118 10.00 6.00 2.882 236 tagctagcatcgatcgatcagt 138 22 0 45.45 59.118 10.00 6.00 2.882 237 atcgcgcagagatgatagtagc 377 22 0 50.00 60.887 4.00 3.00 2.887 238 gatcgcgcagagatgatagta 378 21 0 47.62 58.106 4.00 3.00 2.894 239 agctagcatcgatcgatcagtc 137 22 0 50.00 60.895 10.00 6.00 2.895 240 atcgatcgatcagtcagctagc 130 22 0 50.00 60.895 10.00 6.00 2.895 241 gctagtagcatgcatgcatc 469 20 0 50.00 57.086 10.00 8.00 2.914 242 gctagcatcgatcgatcagct 417 21 0 52.38 61.918 10.00 8.00 2.918 243 agcatcgatcgatcagctagc 414 21 0 52.38 61.918 10.00 7.00 2.918 244 gcatcgatcgatcagctagct 413 21 0 52.38 61.918 10.00 8.00 2.918 245 gcgctagctagtagcccag 691 19 0 63.16 58.067 8.00 5.00 2.933 246 cgctagctagtagcccagc 690 19 0 63.16 58.067 8.00 7.00 2.933 247 gatgatcgatcagtcagctagc 282 22 0 50.00 59.062 10.00 6.00 2.938 248 tcgatcgcgcagagatgata 380 20 0 50.00 62.940 6.00 2.00 2.940 249 agctagctagccgcacatcg 107 20 0 60.00 62.979 10.00 2.00 2.979 250 ctagctagcatcgcgtatcag 497 21 0 52.38 58.020 8.00 2.00 2.980 251 tagcccagctagctagcatcg 680 21 0 57.14 61.990 10.00 8.00 2.990 252 ctagctagatcgccgctacga 596 21 0 57.14 61.990 8.00 4.00 2.990 253 cgatcgatcagctagctagcat 578 22 0 50.00 61.005 10.00 8.00 3.005 254 agctagctagcatcgatgatcg 569 22 0 50.00 61.005 10.00 8.00 3.005 255 tagctagctagcatcgcgtatc 500 22 0 50.00 58.987 10.00 2.00 3.013 256 gatgatcgatcagtcagctagct 282 23 0 47.83 60.025 10.00 8.00 3.025 257 actagctagcatcgatcgatcag 140 23 0 47.83 60.041 10.00 8.00 3.041 258 ctagctagcatcgatcgatcagt 139 23 0 47.83 60.041 10.00 6.00 3.041 259 agctagtagcatcgatgatcagc 217 23 0 47.83 60.048 8.00 8.00 3.048 260 gctagtagcatcgatgatcagct 216 23 0 47.83 60.048 8.00 7.00 3.048 261 agtagcatcgatgatcagctagc 213 23 0 47.83 60.048 8.00 6.00 3.048 262 gtagcatcgatgatcagctagct 212 23 0 47.83 60.048 8.00 8.00 3.048 263 agctagcatactagcatcgatcg 350 23 0 47.83 60.063 10.00 6.00 3.063 264 tagctagctacgatcgatcagc 625 22 0 50.00 58.927 10.00 6.00 3.073 265 gctacgatcgatcagctagcta 582 22 0 50.00 58.927 10.00 6.00 3.073 266 gctactagctagcatcgatcga 143 22 0 50.00 58.927 8.00 8.00 3.073 267 catcgatcgcgcagagat 382 18 0 55.56 61.080 6.00 4.00 3.080 268 atcgatcgcgcagagatg 381 18 0 55.56 61.080 6.00 2.00 3.080 269 cgatcgcgcagagatgat 379 18 0 55.56 61.080 6.00 3.00 3.080 270 agcatcgatcgatgtagca 338 19 0 47.37 57.890 12.00 3.00 3.110 271 cagctagctagatcgccgcta 599 21 0 57.14 62.111 8.00 4.00 3.111 272 tagctagcatcgcgtatcagt 496 21 0 47.62 57.875 6.00 1.00 3.125 273 gctagcatcgcctagctagca 431 21 0 57.14 62.125 9.00 5.00 3.125 274 ctagctagctagcatcgcgta 501 21 0 52.38 57.862 12.00 6.00 3.138 275 agcatcgcctagctagcat 428 19 0 52.63 57.861 8.00 6.00 3.139 276 ctagctagcatcgcgtatcagt 497 22 0 50.00 58.860 8.00 1.00 3.140 277 ctagctagctagcatcgcgtatc 501 23 0 52.17 59.860 12.00 2.00 3.140 278 tcagctagctagtagcatgcatg 476 23 0 47.83 59.856 8.00 8.00 3.144 279 tagtcagctagctagatcgccg 603 22 0 54.55 61.145 8.00 6.00 3.145 280 gctagctagccgcacatcg 106 19 0 63.16 62.150 10.00 2.00 3.150 281 tagtagcatgcatgcatcgatc 467 22 0 45.45 61.154 10.00 6.00 3.154 282 gcgcagagatgatagtagctagc 374 23 0 52.17 59.838 6.00 6.00 3.162 283 ctagctagctacgatcgatcagc 626 23 0 52.17 59.832 10.00 6.00 3.168 284 tagctagctacgatcgatcagct 625 23 0 47.83 59.832 10.00 8.00 3.168 285 gctacgatcgatcagctagctag 582 23 0 52.17 59.832 10.00 8.00 3.168 286 agctactagctagcatcgatcga 144 23 0 47.83 59.832 9.00 8.00 3.168 287 agcatcgatgatcagctagctag 210 23 0 47.83 60.170 8.00 8.00 3.170 288 tgatcgatcagtcagctagct 280 21 0 47.62 57.809 10.00 8.00 3.191 289 gatcgatcagctagtcagctagc 614 23 0 52.17 59.808 8.00 7.00 3.192 290 tagctagcatcgatcgcg 389 18 0 55.56 58.807 6.00 4.00 3.193 291 agcatcgatcgatcagtca 133 19 0 47.37 57.768 10.00 2.00 3.232 292 gcatcgatcgatcagtcag 132 19 0 52.63 57.768 10.00 2.00 3.232 293 catcgatcgatcagtcagc 131 19 0 52.63 57.768 10.00 2.00 3.232 294 gctagctagcatcgtcagcta 673 21 0 52.38 57.757 10.00 6.00 3.243 295 tagctagcatcgtcagctagc 671 21 0 52.38 57.757 7.00 7.00 3.243 296 gctagcatcgtcagctagcta 669 21 0 52.38 57.757 8.00 6.00 3.243 297 agctagctagcatcgcctagct 436 22 0 54.55 61.245 10.00 8.00 3.245 298 agtagctagctagcatcgcct 439 21 0 52.38 57.752 10.00 4.00 3.248 299 catgatgatcgatcagtcagcta 285 23 0 43.48 60.250 12.00 5.00 3.250 300 agctagctagcatcgtcagcta 674 22 0 50.00 58.749 10.00 6.00 3.251 301 gctagctagcatcgtcagctag 673 22 0 54.55 58.749 10.00 8.00 3.251 302 ctagctagcatcgtcagctagc 672 22 0 54.55 58.749 8.00 7.00 3.251 303 agctagcatcgtcagctagcta 670 22 0 50.00 58.749 9.00 6.00 3.251 304 gctagcatcgtcagctagctag 669 22 0 54.55 58.749 8.00 8.00 3.251 305 cgcgtatcagtcagctagctagt 486 23 0 52.17 60.254 8.00 6.00 3.254 306 agctagcatgatgatcgatcagt 291 23 0 43.48 60.271 10.00 8.00 3.271 307 ccgctacgatcgatcagctag 584 21 0 57.14 62.273 8.00 8.00 3.273 308 cgctacgatcgatcagcta 583 19 0 52.63 57.716 8.00 6.00 3.284 309 ctagctagccgcacatcgatc 105 21 0 57.14 62.288 8.00 4.00 3.288 310 tcgactagctagctacgatcgat 630 23 0 47.83 59.706 12.00 8.00 3.294 311 cgactagctagctacgatcgatc 629 23 0 52.17 59.706 10.00 8.00 3.294 312 agctagctagcatcgtcagctag 674 23 0 52.17 59.657 10.00 8.00 3.343 313 agctagcatcgtcagctagctag 670 23 0 52.17 59.657 9.00 8.00 3.343 314 cagctagctagcatcgtcagct 675 22 0 54.55 61.343 10.00 4.00 3.343 315 tagatcgccgctacgatcg 591 19 0 57.89 62.347 8.00 6.00 3.347 316 tcgatcagctagtcagctagc 612 21 0 52.38 57.646 7.00 7.00 3.354 317 catgcatcgatcgatgatagta 457 22 0 40.91 58.629 12.00 7.00 3.371 318 cgactagctagctacgatcga 629 21 0 52.38 57.613 10.00 6.00 3.387 319 agtagcccagctagctagcat 682 21 0 52.38 57.612 10.00 8.00 3.388 320 catgcatcgatcgatgatagtag 457 23 0 43.48 59.605 12.00 7.00 3.395 321 agcatcgcgtatcagtcagct 491 21 0 52.38 62.397 4.00 4.00 3.397 322 catgcatgcatcgatcga 461 18 0 50.00 61.413 10.00 8.00 3.413 323 tagcatcgcctagctagca 429 19 0 52.63 57.552 8.00 4.00 3.448 324 gcgctagctagtagcccagct 691 21 0 61.90 62.455 8.00 5.00 3.455 325 atgcatcgatcgatgatagtagc 456 23 0 43.48 60.469 12.00 4.00 3.469 326 agcatcgcgtatcagtcag 491 19 0 52.63 57.520 4.00 1.00 3.480 327 catcgcgtatcagtcagct 489 19 0 52.63 57.520 4.00 4.00 3.480 328 tagctagcatcgatgatgatca 539 22 0 40.91 58.509 8.00 7.00 3.491 329 tagcatcgatgatgatcagcta 535 22 0 40.91 58.509 8.00 8.00 3.491 330 agctagctagcatcgcgtatca 499 22 0 50.00 61.508 10.00 1.00 3.508 331 gctagctagcatcgcgtatcag 498 22 0 54.55 61.508 10.00 2.00 3.508 332 ctagctagcatcgatgatgatca 540 23 0 43.48 59.492 8.00 7.00 3.508 333 tagctagcatcgatgatgatcag 539 23 0 43.48 59.492 8.00 6.00 3.508 334 ctagcatcgatgatgatcagcta 536 23 0 43.48 59.492 8.00 8.00 3.508 335 tagcatcgatgatgatcagctag 535 23 0 43.48 59.492 8.00 5.00 3.508 336 catcgatgatgatcagctagcta 532 23 0 43.48 59.492 8.00 6.00 3.508 337 tagctagcatgatgatcgatcag 292 23 0 43.48 59.492 10.00 8.00 3.508 338 agctagctagatcgccgc 598 18 0 61.11 58.478 8.00 3.00 3.522 339 gctagctagatcgccgct 597 18 0 61.11 58.478 8.00 3.00 3.522 340 ctagccgcacatcgatcg 101 18 0 61.11 61.523 6.00 6.00 3.523 341 gcatcgatgatcgatagctact 560 22 0 45.45 58.470 11.00 4.00 3.530 342 gcatcgatcgatgatagtagct 454 22 0 45.45 58.470 12.00 4.00 3.530 343 acgatcgatcagctagctagca 579 22 0 50.00 61.537 10.00 8.00 3.537 344 tcgcgcagagatgatagtagct 376 22 0 50.00 61.537 4.00 4.00 3.537 345 agctacgatcgatcagctagtca 620 23 0 47.83 60.556 8.00 5.00 3.556 346 gctacgatcgatcagctagtcag 619 23 0 52.17 60.556 8.00 4.00 3.556 347 ctacgatcgatcagctagtcagc 618 23 0 52.17 60.556 8.00 3.00 3.556 348 tacgatcgatcagctagtcagct 617 23 0 47.83 60.556 8.00 5.00 3.556 349 acgatcgatcagctagtcagcta 616 23 0 47.83 60.556 8.00 3.00 3.556 350 gcatgatcgactagctagctacg 636 23 0 52.17 60.560 10.00 8.00 3.560 351 ctagctagcatcgcgtatcagtc 497 23 0 52.17 60.560 8.00 1.00 3.560 352 atcgcgtatcagtcagctagcta 488 23 0 47.83 60.560 8.00 6.00 3.560 353 gcatcgatgatcgatagctac 560 21 0 47.62 57.434 11.00 4.00 3.566 354 gcatcgatcgatgatagtagc 454 21 0 47.62 57.434 12.00 4.00 3.566 355 agcccagctagctagcatc 679 19 0 57.89 57.433 10.00 6.00 3.567 356 tcgatcgatcagtcagctagct 129 22 0 50.00 61.569 8.00 8.00 3.569 357 atgcgctagctagtagccc 693 19 0 57.89 57.405 8.00 8.00 3.595 358 atcagctagctagcatcgatg 572 21 0 47.62 57.405 10.00 8.00 3.595 359 cagctagctagcatcgatgat 570 21 0 47.62 57.405 10.00 8.00 3.595 360 tagtagcccagctagctagcatc 683 23 0 52.17 59.387 10.00 6.00 3.613 361 tagctagcatcgatcgcgc 389 19 0 57.89 62.627 6.00 4.00 3.627 362 tcgcctagctagcatcga 424 18 0 55.56 58.362 8.00 4.00 3.638 363 cgcgtatcagtcagctagc 486 19 0 57.89 57.357 6.00 6.00 3.643 364 gatcgatcagctagctagcatc 577 22 0 50.00 58.355 10.00 8.00 3.645 365 cgcgcagagatgatagtagctag 375 23 0 52.17 60.675 6.00 4.00 3.675 366 cgatcgatcagctagtcagctag 615 23 0 52.17 60.675 8.00 5.00 3.675 367 cgatcgatcagtcagctagctag 128 23 0 52.17 60.675 8.00 8.00 3.675 368 tagtagcccagctagctagca 683 21 0 52.38 57.324 10.00 8.00 3.676 369 ctagtagcccagctagctagca 684 22 0 54.55 58.309 10.00 8.00 3.691 370 atcgccgctacgatcgat 588 18 0 55.56 61.698 6.00 6.00 3.698 371 ctagctagccgcacatcg 105 18 0 61.11 58.288 8.00 2.00 3.712 372 tgatcgatcagtcagctagctaa 280 23 0 43.48 59.284 10.00 8.00 3.716 373 cgatcgcgcagagatgatagt 379 21 0 52.38 62.718 6.00 1.00 3.718 374 cctagctagcatcgatcgatca 421 22 0 50.00 61.720 10.00 6.00 3.720 375 atgatgatcgatcagtcagct 284 21 0 42.86 57.267 12.00 6.00 3.733 376 ctagctagctagcatcgatcg 394 21 0 52.38 57.266 12.00 6.00 3.734 377 tagctagtagcatgcatgcatc 471 22 0 45.45 58.265 10.00 8.00 3.735 378 agcccagctagctagcatcgt 679 21 0 57.14 62.744 10.00 6.00 3.744 379 atcgcgcagagatgatagtag 377 21 0 47.62 57.255 4.00 1.00 3.745 380 atcgatgtagcatcgatcagt 331 21 0 42.86 57.244 12.00 2.00 3.756 381 tagctagctagcatcgcgtat 500 21 0 47.62 57.241 10.00 4.00 3.759 382 ctagctagtagcatgcatgcatc 472 23 0 47.83 59.227 10.00 8.00 3.773 383 ctagctagctagcatcgcgtat 501 22 0 50.00 58.212 12.00 4.00 3.788 384 ctagctagctagccgcaca 109 19 0 57.89 57.208 12.00 1.00 3.792 385 cgatgtagcatcgatcagtagct 329 23 0 47.83 60.793 8.00 4.00 3.793 386 atgatcgatcagtcagctagct 281 22 0 45.45 58.201 10.00 8.00 3.799 387 ctagcatcgatcgcgcag 386 18 0 61.11 61.813 6.00 4.00 3.813 388 agcatcgatcgatcagtcagc 133 21 0 52.38 62.815 10.00 2.00 3.815 389 gcatcgatcgatcagtcagct 132 21 0 52.38 62.815 10.00 4.00 3.815 390 agcatcgatgatgatcagc 534 19 0 47.37 57.176 8.00 7.00 3.824 391 gcatcgatgatgatcagct 533 19 0 47.37 57.176 8.00 5.00 3.824 392 cccagctagctagcatcgtca 677 21 0 57.14 62.839 10.00 2.00 3.839 393 atcgatcgatgtagcatcg 335 19 0 47.37 57.160 10.00 8.00 3.840 394 tactagctagcatcgatcgatca 141 23 0 43.48 59.159 10.00 6.00 3.841 395 gcatcgatcgatgtagcat 337 19 0 47.37 57.155 12.00 4.00 3.845 396 tagcatcgatcgatcagctagc 415 22 0 50.00 61.859 10.00 8.00 3.859 397 gcatcgatcgatcagctagcta 413 22 0 50.00 61.859 10.00 6.00 3.859 398 gctagcatactagcatcgatcga 349 23 0 47.83 60.866 10.00 8.00 3.866 399 tagctagcatcgatcgatcagtc 138 23 0 47.83 60.873 10.00 6.00 3.873 400 agctactagctagcatcgatcg 144 22 0 50.00 58.126 9.00 6.00 3.874 401 atcgatcgatcagtcagctag 130 21 0 47.62 57.122 10.00 4.00 3.878 402 tagctagctagccgcacatcg 108 21 0 57.14 62.882 10.00 2.00 3.882 403 atgatcgatcagtcagctagc 281 21 0 47.62 57.117 10.00 6.00 3.883 404 gctactagctagcatcgatcg 143 21 0 52.38 57.116 8.00 6.00 3.884 405 cagctagctagtagcatgcatg 475 22 0 50.00 58.085 8.00 8.00 3.915 406 tgatgatcgatcagtcagctag 283 22 0 45.45 58.076 12.00 4.00 3.924 407 atcgatcagctagtcagctagc 613 22 0 50.00 58.034 7.00 7.00 3.966 408 ctagctagtagcatgcatgca 472 21 0 47.62 57.032 10.00 8.00 3.968 409 tcgatgtagcatcgatcagtag 330 22 0 45.45 58.026 10.00 0.00 3.974 410 agctagcatcgatcgatca 418 19 0 47.37 57.026 10.00 6.00 3.974 411 gctagcatcgatcgatcag 417 19 0 52.63 57.026 10.00 8.00 3.974 412 ctagcatcgatcgatcagc 416 19 0 52.63 57.026 10.00 6.00 3.974 413 tagcatcgatcgatcagct 415 19 0 47.37 57.026 10.00 8.00 3.974 414 agcatcgatcgatcagcta 414 19 0 47.37 57.026 10.00 6.00 3.974 415 gcatcgatcgatcagctag 413 19 0 52.63 57.026 10.00 5.00 3.974 416 catcgatcgatcagctagc 412 19 0 52.63 57.026 10.00 6.00 3.974 417 gatcgcgcagagatgatag 378 19 0 52.63 57.026 4.00 0.00 3.974 418 agctagcatcgatcgatca 137 19 0 47.37 57.026 10.00 6.00 3.974 419 gctagcatcgatcgatcag 136 19 0 52.63 57.026 10.00 8.00 3.974 420 gcatcgatgatcagctagc 209 19 0 52.63 57.021 8.00 6.00 3.979 421 gatcagctagctagcatcgatga 573 23 0 47.83 61.000 10.00 8.00 4.000 422 tcagctagctagcatcgatgatc 571 23 0 47.83 61.000 10.00 6.00 4.000 423 cgactagctagctacgatcgat 629 22 0 50.00 57.990 10.00 6.00 4.010 424 tgatcagctagctagtagcatcg 226 23 0 47.83 58.981 8.00 6.00 4.019 425 tcagctagctagtagcatcgatg 223 23 0 47.83 58.981 8.00 8.00 4.019 426 cagctagctagtagcatcgatga 222 23 0 47.83 58.981 8.00 6.00 4.019 427 gatgatcgatcagtcagctagcta 282 24 0 45.83 60.034 10.00 6.00 4.034 428 ctactagctagcatcgatcgatca 142 24 0 45.83 60.049 10.00 6.00 4.049 429 tactagctagcatcgatcgatcag 141 24 0 45.83 60.049 10.00 8.00 4.049 430 gctactagctagcatcgatgatga 544 24 0 45.83 60.056 8.00 6.00 4.056 431 gctagtagctagcatgatgatcga 297 24 0 45.83 60.056 8.00 4.00 4.056 432 gctagctagtagcatcgatgatca 220 24 0 45.83 60.056 8.00 6.00 4.056 433 tagctagtagcatcgatgatcagc 218 24 0 45.83 60.056 8.00 8.00 4.056 434 gctagtagcatcgatgatcagcta 216 24 0 45.83 60.056 8.00 8.00 4.056 435 tagtagcatcgatgatcagctagc 214 24 0 45.83 60.056 8.00 6.00 4.056 436 tagctagcatactagcatcgatcg 351 24 0 45.83 60.070 10.00 6.00 4.070 437 gatagtagctagctagcatcgcc 442 23 0 52.17 58.921 12.00 6.00 4.079 438 tgatcgatcagtcagctagcta 280 22 0 45.45 57.905 10.00 6.00 4.095 439 tgcatcgatcgatgatagtagct 455 23 0 43.48 61.109 12.00 4.00 4.109 440 gtagctagctagcatcgcctagc 438 23 0 56.52 61.109 10.00 6.00 4.109 441 agctacgatcgatcagctagtc 620 22 0 50.00 57.891 8.00 6.00 4.109 442 gctagtcagctagctagatcgcc 605 23 0 56.52 61.123 8.00 6.00 4.123 443 gctagtcagctagctagatcgc 605 22 0 54.55 57.876 8.00 8.00 4.124 444 ctagctagatcgccgctacgat 596 22 0 54.55 62.138 8.00 6.00 4.138 445 tgtagcatcgatcagtagctagc 326 23 0 47.83 58.849 6.00 6.00 4.151 446 tagctactagctagcatcgatcga 145 24 0 45.83 59.847 11.00 8.00 4.153 447 catgatcgactagctagctacga 635 23 0 47.83 58.847 10.00 8.00 4.153 448 gctagcatcgatgatgatcagc 537 22 0 50.00 62.158 8.00 8.00 4.158 449 gcatcgatgatgatcagctagc 533 22 0 50.00 62.158 8.00 7.00 4.158 450 tagtagctagctagcatcgcct 440 22 0 50.00 57.839 10.00 4.00 4.161 451 agtagctagctagcatcgccta 439 22 0 50.00 57.839 10.00 6.00 4.161 452 gtagctagctagcatcgcctag 438 22 0 54.55 57.839 10.00 4.00 4.161 453 tagcatcgatgatcagctagctag 211 24 0 45.83 60.174 10.00 8.00 4.174 454 agctagtcagctagctagatcgc 606 23 0 52.17 58.820 9.00 8.00 4.180 455 tcgcgtatcagtcagctagctag 487 23 0 52.17 61.184 8.00 8.00 4.184 456 tagctagctagcatcgcctagct 437 23 0 52.17 61.214 10.00 8.00 4.214 457 ctagtagcccagctagctagcatc 684 24 0 54.17 60.219 10.00 6.00 4.219 458 gatagtagctagctagcatcgcct 442 24 0 50.00 59.764 12.00 4.00 4.236 459 tgtagcatcgatcagtagctagct 326 24 0 45.83 59.754 8.00 8.00 4.246 460 agtagctagctagcatcgcctag 439 23 0 52.17 58.754 10.00 4.00 4.246 461 cgcgtatcagtcagctagctagta 486 24 0 50.00 60.255 8.00 4.00 4.255 462 gtagcatgcatgcatcga 465 18 0 50.00 57.745 10.00 8.00 4.255 463 ctagtcagctagctagatcgcc 604 22 0 54.55 57.739 8.00 6.00 4.261 464 tcgatcagctagtcagctagcta 612 23 0 47.83 58.736 9.00 7.00 4.264 465 gctagcatcgcctagctagcat 431 22 0 54.55 62.268 9.00 6.00 4.268 466 gcatgcatcgatcgatgatagt 458 22 0 45.45 62.270 12.00 8.00 4.270 467 actagctagcatcgatgatgatca 541 24 0 41.67 60.271 8.00 7.00 4.271 468 gtagctagcatgatgatcgatcag 293 24 0 45.83 60.271 10.00 8.00 4.271 469 tagctagcatgatgatcgatcagt 292 24 0 41.67 60.271 10.00 8.00 4.271 470 tagtagcccagctagctagcat 683 22 0 50.00 57.705 10.00 8.00 4.295 471 cagctagctagcatcgtcagcta 675 23 0 52.17 61.306 10.00 6.00 4.306 472 gtcagctagctagtagcatgca 477 22 0 50.00 57.681 8.00 6.00 4.319 473 agctagcatcgcgtatcagtca 495 22 0 50.00 62.319 6.00 1.00 4.319 474 gctagcatcgcgtatcagtcag 494 22 0 54.55 62.319 6.00 1.00 4.319 475 ctagcatcgcgtatcagtcagc 493 22 0 54.55 62.319 4.00 2.00 4.319 476 tagcatcgcgtatcagtcagct 492 22 0 50.00 62.319 4.00 4.00 4.319 477 agcatcgcgtatcagtcagcta 491 22 0 50.00 62.319 4.00 3.00 4.319 478 gcatcgcgtatcagtcagctag 490 22 0 54.55 62.319 4.00 4.00 4.319 479 catcgcgtatcagtcagctagc 489 22 0 54.55 62.319 6.00 6.00 4.319 480 tagctagcatcgtcagctagctag 671 24 0 50.00 59.677 11.00 8.00 4.323 481 cagtcagctagctagtagcatgc 479 23 0 52.17 58.672 8.00 7.00 4.328 482 agtcagctagctagtagcatgca 478 23 0 47.83 58.672 8.00 6.00 4.328 483 tcgatcagctagtcagctagctag 612 24 0 50.00 59.644 9.00 8.00 4.356 484 tcagctagtcagctagctagatcg 608 24 0 50.00 59.644 9.00 6.00 4.356 485 ctagtagcccagctagctagcat 684 23 0 52.17 58.628 10.00 8.00 4.372 486 tcagtcagctagctagtagcatgc 480 24 0 50.00 60.373 8.00 7.00 4.373 487 gcgctagctagtagcccagcta 691 22 0 59.09 62.383 8.00 6.00 4.383 488 ctagctagcatcgatgatgatcag 540 24 0 45.83 60.391 8.00 6.00 4.391 489 catcgatgatgatcagctagctag 532 24 0 45.83 60.391 8.00 8.00 4.391 490 cgctacgatcgatcagct 583 18 0 55.56 57.606 8.00 4.00 4.394 491 atgatcgatcagtcagctagctaa 281 24 0 41.67 59.570 10.00 8.00 4.430 492 agatcgccgctacgatcg 590 18 0 61.11 62.431 8.00 6.00 4.431 493 gcatcgatgatcgatagctacta 560 23 0 43.48 58.534 11.00 4.00 4.466 494 gcatcgatcgatgatagtagcta 454 23 0 43.48 58.534 12.00 6.00 4.466 495 tagctagctagcatcgcgtatca 500 23 0 47.83 61.466 10.00 1.00 4.466 496 tacgatcgatcagctagctagca 580 23 0 47.83 61.493 10.00 8.00 4.493 497 tcgcgcagagatgatagtagcta 376 23 0 47.83 61.493 6.00 6.00 4.493 498 gcctagctagcatcgatcgatc 422 22 0 54.55 62.516 10.00 8.00 4.516 499 tcgatcgatcagtcagctagcta 129 23 0 47.83 61.523 8.00 6.00 4.523 500 tagatcgccgctacgatc 591 18 0 55.56 57.475 8.00 8.00 4.525 501 tgatcgatcagtcagctagctaaa 280 24 0 41.67 60.527 10.00 6.00 4.527 502 tagccgcacatcgatcga 100 18 0 55.56 62.543 8.00 8.00 4.543 503 agctagctagtagcatgcatgca 474 23 0 47.83 61.544 10.00 8.00 4.544 504 tagctacgatcgatcagctagtca 621 24 0 45.83 60.546 10.00 6.00 4.546 505 tacgatcgatcagctagtcagcta 617 24 0 45.83 60.546 8.00 3.00 4.546 506 atgatgatcgatcagtcagctag 284 23 0 43.48 58.451 12.00 4.00 4.549 507 actagctagctacgatcgatcagc 627 24 0 50.00 60.553 10.00 6.00 4.553 508 gcatcgatgatcgatagctactag 560 24 0 45.83 59.445 11.00 5.00 4.555 509 catcgatgatcgatagctactagc 559 24 0 45.83 59.445 11.00 7.00 4.555 510 gcatcgatcgatgatagtagctag 454 24 0 45.83 59.445 12.00 4.00 4.555 511 catcgatcgatgatagtagctagc 453 24 0 45.83 59.445 12.00 6.00 4.555 512 cagctagctagatcgccg 599 18 0 61.11 57.442 8.00 6.00 4.558 513 agcatcgcctagctagca 428 18 0 55.56 57.439 8.00 4.00 4.561 514 gctagtagcccagctagctagca 685 23 0 56.52 61.586 11.00 8.00 4.586 515 gtcagctagctagtagcatgcatg 477 24 0 50.00 60.603 8.00 8.00 4.603 516 tcgatgatgatcagctagctag 530 22 0 45.45 57.392 8.00 8.00 4.608 517 atcgatgtagcatcgatcagtag 331 23 0 43.48 58.391 12.00 0.00 4.609 518 atgatgatcgatcagtcagcta 284 22 0 40.91 57.387 12.00 4.00 4.613 519 cgatcgcgcagagatgatagta 379 22 0 50.00 62.629 6.00 3.00 4.629 520 ctagcatactagcatcgatcga 348 22 0 45.45 57.367 8.00 8.00 4.633 521 atcgatgtagcatcgatcagta 331 22 0 40.91 57.360 12.00 2.00 4.640 522 gctactagctagcatcgatgatg 544 23 0 47.83 58.360 8.00 6.00 4.640 523 gctagtagctagcatgatgatcg 297 23 0 47.83 58.360 8.00 5.00 4.640 524 tcgatgtagcatcgatcagtagc 330 23 0 47.83 61.644 10.00 2.00 4.644 525 gatgcgctagctagtagcccag 694 22 0 59.09 62.659 10.00 8.00 4.659 526 gtagcccagctagctagcatcg 681 22 0 59.09 62.659 10.00 8.00 4.659 527 tagcccagctagctagcatcgt 680 22 0 54.55 62.659 10.00 6.00 4.659 528 ctagctagctacgatcgatcagct 626 24 0 50.00 60.663 10.00 8.00 4.663 529 tgatcagctagctagtagcatcga 226 24 0 45.83 60.670 8.00 6.00 4.670 530 gctagcatgatcgactagctagct 640 24 0 50.00 60.672 10.00 8.00 4.672 531 gcgcagagatgatagtagctagct 374 24 0 50.00 60.672 8.00 8.00 4.672 532 agctagtagcatgcatgcatcg 470 22 0 50.00 62.673 10.00 8.00 4.673 533 acgatcgatcagctagctagcat 579 23 0 47.83 61.711 10.00 8.00 4.711 534 atcgcgcagagatgatagtagct 377 23 0 47.83 61.711 4.00 4.00 4.711 535 gctagcatcgatcgatcagtca 136 22 0 50.00 62.719 10.00 4.00 4.719 536 tagcatcgatcgatcagtcagc 134 22 0 50.00 62.719 10.00 2.00 4.719 537 gcatcgatcgatcagtcagcta 132 22 0 50.00 62.719 10.00 2.00 4.719 538 cccagctagctagcatcg 677 18 0 61.11 57.281 10.00 8.00 4.719 539 agctagcatcgatcgcgc 388 18 0 61.11 62.722 6.00 4.00 4.722 540 tcgatgatgatcagctagctagt 530 23 0 43.48 58.277 8.00 6.00 4.723 541 atgatcgatcagtcagctagcta 281 23 0 43.48 58.277 10.00 6.00 4.723 542 agctactagctagcatcgatgatg 545 24 0 45.83 59.274 9.00 6.00 4.726 543 agctagtagctagcatgatgatcg 298 24 0 45.83 59.274 9.00 5.00 4.726 544 atgatcagctagctagtagcatcg 227 24 0 45.83 59.274 8.00 7.00 4.726 545 atcagctagctagtagcatcgatg 224 24 0 45.83 59.274 8.00 8.00 4.726 546 cagctagctagtagcatcgatgat 222 24 0 45.83 59.274 8.00 8.00 4.726 547 gctagctagccgcacatc 106 18 0 61.11 57.273 10.00 0.00 4.727 548 agcatcgatcgatcagctagct 414 22 0 50.00 62.732 10.00 8.00 4.732 549 atcgatcgatcagtcagctagct 130 23 0 47.83 61.747 10.00 8.00 4.747 550 atagtagctagctagcatcgcc 441 22 0 50.00 57.244 10.00 4.00 4.756 551 cagctagctagatcgccgctac 599 22 0 59.09 62.774 8.00 2.00 4.774 552 cgatgtagcatcgatcagtagcta 329 24 0 45.83 60.775 8.00 6.00 4.775 553 actagctagcatcgatcgatcagt 140 24 0 45.83 60.775 10.00 6.00 4.775 554 tgatagtagctagctagcatcgc 443 23 0 47.83 58.200 12.00 8.00 4.800 555 gctagctagcatactagcatcga 353 23 0 47.83 58.200 10.00 7.00 4.800 556 cgatagctactagctagcatcga 549 23 0 47.83 58.200 12.00 8.00 4.800 557 tagctactagctagcatcgatcg 145 23 0 47.83 58.200 11.00 6.00 4.800 558 cagctagctagtagcatcgatg 222 22 0 50.00 57.193 8.00 8.00 4.807 559 tgatgatcgatcagtcagctagc 283 23 0 47.83 61.809 12.00 6.00 4.809 560 tcagctagctagtagcatgcat 476 22 0 45.45 57.189 8.00 6.00 4.811 561 atagtagctagctagcatcgcct 441 23 0 47.83 58.177 10.00 4.00 4.823 562 tagcatcgatcgcgcaga 385 18 0 55.56 62.829 6.00 4.00 4.829 563 gctactagctagcatcgatcgatc 143 24 0 50.00 60.846 10.00 8.00 4.846 564 atgtagcatcgatcagtagctagc 327 24 0 45.83 59.150 6.00 6.00 4.850 565 tagcatgcatgcatcgat 464 18 0 44.44 57.148 10.00 8.00 4.852 566 gatcagctagctagtagcatcga 225 23 0 47.83 58.113 8.00 6.00 4.887 567 agctagcatcgcctagctagca 432 22 0 54.55 62.888 9.00 7.00 4.888 568 agtagcatcgatgatcagctagct 213 24 0 45.83 60.897 8.00 8.00 4.897 569 agctagctagcatactagcatcga 354 24 0 45.83 59.095 10.00 6.00 4.905 570 tcgatcgcgcagagatga 380 18 0 55.56 62.924 6.00 4.00 4.924 571 ctagtcagctagctagatcgccg 604 23 0 56.52 61.940 8.00 6.00 4.940 572 gtcagctagctagtagcatgcat 477 23 0 47.83 58.052 8.00 6.00 4.948 573 catgatcgactagctagctacg 635 22 0 50.00 57.048 10.00 8.00 4.952 574 atcgatcagctagtcagctagcta 613 24 0 45.83 59.042 9.00 7.00 4.958 575 tagctagatcgccgctacgatc 595 22 0 54.55 62.965 8.00 8.00 4.965 576 ctagtagcatgcatgcatcgatc 468 23 0 47.83 62.001 10.00 6.00 5.001 577 atgtagcatcgatcagtagctagct 327 25 0 44.00 60.001 8.00 8.00 5.001 578 agtcagctagctagtagcatgcat 478 24 0 45.83 58.980 8.00 6.00 5.020 579 gactagctagctacgatcgatca 628 23 0 47.83 57.977 10.00 6.00 5.023 580 tagctacgatcgatcagctagtc 621 23 0 47.83 57.977 10.00 6.00 5.023 581 gatcgatcagtcagctagctaaa 279 23 0 43.48 57.951 8.00 6.00 5.049 582 cgatcagtcagctagctaaatagc 276 24 0 45.83 58.950 8.00 6.00 5.050 583 cgatcagctagtcagctagctag 611 23 0 52.17 57.942 9.00 8.00 5.058 584 cagctagtcagctagctagatcg 607 23 0 52.17 57.942 9.00 6.00 5.058 585 tagtagctagctagcatcgccta 440 23 0 47.83 57.921 10.00 6.00 5.079 586 tgcatcgatcgatgatagtagcta 455 24 0 41.67 61.080 12.00 6.00 5.080 587 gactagctagctacgatcgatcag 628 24 0 50.00 58.914 10.00 6.00 5.086 588 ctagctacgatcgatcagctagtc 622 24 0 50.00 58.914 12.00 7.00 5.086 589 gctagctagcatactagcatcgatc 353 25 0 48.00 60.089 10.00 4.00 5.089 590 atcgatcagctagtcagctagctag 613 25 0 48.00 59.895 9.00 8.00 5.105 591 atcagctagtcagctagctagatcg 609 25 0 48.00 59.895 11.00 6.00 5.105 592 catgatgatcgatcagtcagctag 285 24 0 45.83 61.155 12.00 5.00 5.155 593 gctagctagcatcgcgtatcagt 498 23 0 52.17 62.182 10.00 1.00 5.182 594 tagtagctagctagcatcgcctag 440 24 0 50.00 58.803 10.00 5.00 5.197 595 gcatgcatcgatcgatgatagta 458 23 0 43.48 62.198 12.00 6.00 5.198 596 atcagctagctagcatcgatgatc 572 24 0 45.83 61.198 10.00 6.00 5.198 597 atcgatgatgatcagctagctag 531 23 0 43.48 57.785 8.00 8.00 5.215 598 cgatcagtcagctagctaaatagct 276 25 0 44.00 59.785 8.00 8.00 5.215 599 gatagtagctagctagcatcgccta 442 25 0 48.00 59.780 12.00 6.00 5.220 600 cagtcagctagctagtagcatgca 479 24 0 50.00 61.222 8.00 6.00 5.222 601 tagctagcatcgcgtatcagtca 496 23 0 47.83 62.247 6.00 1.00 5.247 602 tagcatcgcgtatcagtcagcta 492 23 0 47.83 62.247 4.00 3.00 5.247 603 gcgtatcagtcagctagctagtagc 485 25 0 52.00 60.268 8.00 8.00 5.268 604 cagctagtcagctagctagatcgc 607 24 0 54.17 61.268 9.00 8.00 5.268 605 tactagctagcatcgatgatgatca 542 25 0 40.00 60.272 8.00 7.00 5.272 606 catcgatcgatgatagtagctagct 453 25 0 44.00 60.282 12.00 8.00 5.282 607 agctagctagcatcgcgtatcag 499 23 0 52.17 62.283 10.00 2.00 5.283 608 gctactagctagcatcgatgatgat 544 25 0 44.00 60.289 8.00 6.00 5.289 609 gctagtagctagcatgatgatcgat 297 25 0 44.00 60.289 8.00 6.00 5.289 610 atgcatcgatcgatgatagtagct 456 24 0 41.67 61.301 12.00 4.00 5.301 611 tgatagtagctagctagcatcgcc 443 24 0 50.00 61.308 12.00 6.00 5.308 612 tcgactagctagctacgatcgatc 630 24 0 50.00 61.329 12.00 8.00 5.329 613 actagctagcatcgatgatgatc 541 23 0 43.48 57.643 8.00 6.00 5.357 614 agtagctagcatgatgatcgatc 294 23 0 43.48 57.643 8.00 8.00 5.357 615 agcatgatcgactagctagctacg 637 24 0 50.00 61.368 10.00 8.00 5.368 616 atcgcgtatcagtcagctagctag 488 24 0 50.00 61.368 8.00 8.00 5.368 617 atcgatgatgatcagctagctagt 531 24 0 41.67 58.612 8.00 6.00 5.388 618 agctacgatcgatcagctagtcag 620 24 0 50.00 61.392 8.00 4.00 5.392 619 ctacgatcgatcagctagtcagct 618 24 0 50.00 61.392 8.00 5.00 5.392 620 acgatcgatcagctagtcagctag 616 24 0 50.00 61.392 8.00 5.00 5.392 621 atgatagtagctagctagcatcgc 444 24 0 45.83 58.519 12.00 8.00 5.481 622 gctagctagcatactagcatcgat 353 24 0 45.83 58.519 10.00 6.00 5.481 623 tcgatcgatgatagtagctagct 451 23 0 43.48 57.502 8.00 8.00 5.498 624 ctactagctagcatcgatcgatc 142 23 0 47.83 57.502 10.00 8.00 5.498 625 gctagctagtagcatcgatgatc 220 23 0 47.83 57.499 8.00 6.00 5.501 626 cctagctagcatcgatcgatcag 421 23 0 52.17 62.515 10.00 8.00 5.515 627 cgtatcagtcagctagctagtagca 484 25 0 48.00 59.481 8.00 6.00 5.519 628 tcgatcagtcagctagctaaatagc 277 25 0 44.00 60.527 8.00 6.00 5.527 629 gatcgcgcagagatgatagtagc 378 23 0 52.17 62.545 4.00 3.00 5.545 630 gatcagctagctagtagcatcgat 225 24 0 45.83 58.447 8.00 8.00 5.553 631 agctagctagtagcatcgatgatc 221 24 0 45.83 58.447 8.00 6.00 5.553 632 agctagctagcatactagcatcg 354 23 0 47.83 57.446 10.00 8.00 5.554 633 tagctagtagcatgcatgcatcg 471 23 0 47.83 62.589 10.00 8.00 5.589 634 atcagtcagctagctagtagcatgc 481 25 0 48.00 60.594 8.00 7.00 5.594 635 agctagctagcatactagcatcgat 354 25 0 44.00 59.362 10.00 8.00 5.638 636 cgatcgatcagctagctagcatc 578 23 0 52.17 62.647 10.00 8.00 5.647 637 agcatcgatcgatcagctagcta 414 23 0 47.83 62.647 10.00 6.00 5.647 638 gcatcgatcgatcagctagctag 413 23 0 52.17 62.647 10.00 8.00 5.647 639 tcgatgatgatcagctagctagta 530 24 0 41.67 58.348 8.00 4.00 5.652 640 gatcgatcagctagtcagctagcta 614 25 0 48.00 60.659 9.00 7.00 5.659 641 tacgatcgatcagctagctagcat 580 24 0 45.83 61.663 10.00 8.00 5.663 642 atcgcgcagagatgatagtagcta 377 24 0 45.83 61.663 6.00 6.00 5.663 643 agctagcatactagcatcgatcga 350 24 0 45.83 61.663 10.00 8.00 5.663 644 ctagctagctagcatgatcgact 647 23 0 47.83 57.335 12.00 4.00 5.665 645 agctagctagcatgatcgactag 645 23 0 47.83 57.335 10.00 8.00 5.665 646 agctagcatgatcgactagctag 641 23 0 47.83 57.335 9.00 8.00 5.665 647 ctagcatgatcgactagctagct 639 23 0 47.83 57.335 8.00 8.00 5.665 648 cgcagagatgatagtagctagct 373 23 0 47.83 57.335 8.00 8.00 5.665 649 agcatcgatcagtagctagctag 323 23 0 47.83 57.335 10.00 8.00 5.665 650 tagctactagctagcatcgatgatg 546 25 0 44.00 59.307 11.00 6.00 5.693 651 tagctagtagctagcatgatgatcg 299 25 0 44.00 59.307 11.00 5.00 5.693 652 ctagctagcatcgatcgatcagtc 139 24 0 50.00 61.697 10.00 6.00 5.697 653 atcgatcgatcagtcagctagcta 130 24 0 45.83 61.697 10.00 6.00 5.697 654 gatcgatcagtcagctagctaaat 279 24 0 41.67 58.295 8.00 8.00 5.705 655 gatgatgatcagctagctagtagca 231 25 0 44.00 59.265 8.00 6.00 5.735 656 tcgatgatgatcagctagctagtag 530 25 0 44.00 59.261 8.00 6.00 5.739 657 atgatcgatcagtcagctagctaaa 281 25 0 40.00 60.741 10.00 6.00 5.741 658 tgatcgatcagtcagctagctaaat 280 25 0 40.00 60.741 10.00 8.00 5.741 659 gctagtagcccagctagctagcat 685 24 0 54.17 61.742 11.00 8.00 5.742 660 atagtagctagctagcatcgccta 441 24 0 45.83 58.244 10.00 6.00 5.756 661 tactagctagcatcgatcgatcagt 141 25 0 44.00 60.759 10.00 6.00 5.759 662 gatgtagcatcgatcagtagctagc 328 25 0 48.00 60.768 6.00 6.00 5.768 663 tagctagcatcgcctagctagca 433 23 0 52.17 62.802 10.00 7.00 5.802 664 actagctagctacgatcgatcag 627 23 0 47.83 57.197 10.00 6.00 5.803 665 ctagctacgatcgatcagctagt 622 23 0 47.83 57.197 12.00 8.00 5.803 666 agcatgatcgactagctagctac 637 23 0 47.83 57.193 10.00 8.00 5.807 667 gtagcatcgatcagtagctagct 325 23 0 47.83 57.193 8.00 8.00 5.807 668 atcgatgtagcatcgatcagtagc 331 24 0 45.83 61.812 12.00 2.00 5.812 669 tgatgatcagctagctagtagca 229 23 0 43.48 57.177 8.00 6.00 5.823 670 agtagctagctagcatcgcctagc 439 24 0 54.17 61.851 10.00 6.00 5.851 671 gtagctagctagcatcgcctagct 438 24 0 54.17 61.851 10.00 8.00 5.851 672 cgatagctactagctagcatcgatg 549 25 0 48.00 60.861 12.00 8.00 5.861 673 ctagctagcatactagcatcgatcg 352 25 0 48.00 60.861 10.00 6.00 5.861 674 tagctagctagcatactagcatcga 355 25 0 44.00 59.133 10.00 8.00 5.867 675 ctactagctagcatcgatcgatcag 142 25 0 48.00 60.867 10.00 8.00 5.867 676 tcgcgtatcagtcagctagctagt 487 24 0 50.00 61.869 8.00 6.00 5.869 677 agctactagctagcatcgatgatga 545 25 0 44.00 60.876 9.00 6.00 5.876 678 cgatgatgatcagctagctagtagc 529 25 0 48.00 60.876 8.00 8.00 5.876 679 agctagtagctagcatgatgatcga 298 25 0 44.00 60.876 9.00 4.00 5.876 680 gatgatcagctagctagtagcatcg 228 25 0 48.00 60.876 10.00 8.00 5.876 681 atgatcagctagctagtagcatcga 227 25 0 44.00 60.876 8.00 7.00 5.876 682 tgatcagctagctagtagcatcgat 226 25 0 44.00 60.876 8.00 8.00 5.876 683 gatcagctagctagtagcatcgatg 225 25 0 48.00 60.876 8.00 8.00 5.876 684 tcagctagctagtagcatcgatgat 223 25 0 44.00 60.876 9.00 8.00 5.876 685 cagctagctagtagcatcgatgatc 222 25 0 48.00 60.876 8.00 6.00 5.876 686 agctagctagtagcatcgatgatca 221 25 0 44.00 60.876 8.00 6.00 5.876 687 gctagctagtagcatcgatgatcag 220 25 0 48.00 60.876 8.00 6.00 5.876 688 ctagctagtagcatcgatgatcagc 219 25 0 48.00 60.876 8.00 8.00 5.876 689 ctagtagcatcgatgatcagctagc 215 25 0 48.00 60.876 10.00 6.00 5.876 690 tagtagcatcgatgatcagctagct 214 25 0 44.00 60.876 8.00 8.00 5.876 691 gtagcatcgatgatcagctagctag 212 25 0 48.00 60.876 10.00 8.00 5.876 692 agctagtcagctagctagatcgcc 606 24 0 54.17 61.888 9.00 6.00 5.888 693 atagtagctagctagcatcgcctag 441 25 0 48.00 59.075 10.00 4.00 5.925 694 agctagcatcgatgatgatcagc 538 23 0 47.83 62.960 8.00 8.00 5.960 695 gctagcatcgatgatgatcagct 537 23 0 47.83 62.960 8.00 6.00 5.960 696 agcatcgatgatgatcagctagc 534 23 0 47.83 62.960 8.00 7.00 5.960 697 gcatcgatgatgatcagctagct 533 23 0 47.83 62.960 8.00 8.00 5.960 698 atgatgatcgatcagtcagctagc 284 24 0 45.83 61.975 12.00 6.00 5.975 699 agctagcatcgcctagctagcat 432 23 0 52.17 62.989 9.00 7.00 5.989 700 gtagctagcatgatgatcgatcagt 293 25 0 44.00 60.996 10.00 8.00 5.996 701 cgcgtatcagtcagctagctagtag 486 25 0 52.00 61.048 8.00 6.00 6.048 702 ctagctagctagcatactagcatcga 356 26 0 46.15 59.931 12.00 8.00 6.069 703 cagctagctagcatcgtcagctag 675 24 0 54.17 62.086 10.00 8.00 6.086 704 actagctagcatcgatgatgatcag 541 25 0 44.00 61.106 8.00 6.00 6.106 705 catcgatgatgatcagctagctagt 532 25 0 44.00 61.106 8.00 6.00 6.106 706 agtagctagcatgatgatcgatcag 294 25 0 44.00 61.106 10.00 8.00 6.106 707 agatgatgatcagctagctagtagca 232 26 0 42.31 60.108 9.00 6.00 6.108 708 atcgatcgatgatagtagctagct 452 24 0 41.67 57.861 10.00 8.00 6.139 709 cgactagctagctacgatcgatca 629 24 0 50.00 62.155 10.00 6.00 6.155 710 gcatgatcgactagctagctacga 636 24 0 50.00 62.168 10.00 8.00 6.168 711 gatgatcgatcagtcagctagctaa 282 25 0 44.00 61.208 10.00 8.00 6.208 712 ctagctagctagcatcgcgtatca 501 24 0 50.00 62.218 12.00 1.00 6.218 713 tagctagctagcatcgcgtatcag 500 24 0 50.00 62.218 10.00 2.00 6.218 714 tactagctagcatcgatgatgatc 542 24 0 41.67 57.735 8.00 6.00 6.265 715 tagtagctagcatgatgatcgatc 295 24 0 41.67 57.735 8.00 8.00 6.265 716 atgcatcgatcgatgatagtagcta 456 25 0 40.00 61.267 12.00 6.00 6.267 717 cgtatcagtcagctagctagtagcat 484 26 0 46.15 59.732 8.00 6.00 6.268 718 ctacgatcgatcagctagctagca 581 24 0 50.00 62.269 10.00 8.00 6.269 719 tcgcgcagagatgatagtagctag 376 24 0 50.00 62.269 6.00 4.00 6.269 720 tcgatcgatcagtcagctagctag 129 24 0 50.00 62.323 8.00 8.00 6.323 721 actagctagctacgatcgatcagct 627 25 0 48.00 61.329 10.00 8.00 6.329 722 ctactagctagcatcgatgatgatc 543 25 0 44.00 58.669 8.00 6.00 6.331 723 atcgatgatgatcagctagctagta 531 25 0 40.00 58.669 8.00 4.00 6.331 724 ctagtagctagcatgatgatcgatc 296 25 0 44.00 58.669 8.00 8.00 6.331 725 tagcatgatcgactagctagctacg 638 25 0 48.00 61.333 10.00 8.00 6.333 726 ctagctacgatcgatcagctagtca 622 25 0 48.00 61.355 12.00 7.00 6.355 727 tagctacgatcgatcagctagtcag 621 25 0 48.00 61.355 10.00 4.00 6.355 728 ctacgatcgatcagctagtcagcta 618 25 0 48.00 61.355 8.00 3.00 6.355 729 tacgatcgatcagctagtcagctag 617 25 0 48.00 61.355 8.00 5.00 6.355 730 cagtcagctagctagtagcatgcat 479 25 0 48.00 61.403 8.00 6.00 6.403 731 agtcagctagctagtagcatgcatg 478 25 0 48.00 61.403 8.00 8.00 6.403 732 cgatgatgatcagctagctagtag 529 24 0 45.83 57.570 8.00 6.00 6.430 733 ctagctagtagcatcgatgatcag 219 24 0 45.83 57.570 8.00 6.00 6.430 734 atgatgatcagctagctagtagca 230 24 0 41.67 57.568 8.00 6.00 6.432 735 tgatgatcagctagctagtagcat 229 24 0 41.67 57.568 8.00 8.00 6.432 736 atagctactagctagcatcgatgatg 547 26 0 42.31 59.563 11.00 6.00 6.437 737 tcgatgtagcatcgatcagtagct 330 24 0 45.83 62.441 10.00 4.00 6.441 738 tagctagctagcatactagcatcg 355 24 0 45.83 57.539 10.00 8.00 6.461 739 gatgatgatcagctagctagtagcat 231 26 0 42.31 59.532 8.00 8.00 6.468 740 atcgatgatgatcagctagctagtag 531 26 0 42.31 59.527 8.00 6.00 6.473 741 atgatagtagctagctagcatcgcc 444 25 0 48.00 61.475 12.00 6.00 6.475 742 gatagctactagctagcatcgatga 548 25 0 44.00 58.507 11.00 6.00 6.493 743 tcgatcgatgatagtagctagctag 451 25 0 44.00 58.506 10.00 8.00 6.494 744 gctagctagtagcatgcatgcatc 473 24 0 50.00 62.511 10.00 8.00 6.511 745 gatagtagctagctagcatcgcctag 442 26 0 50.00 60.535 12.00 4.00 6.535 746 ctagctagctagcatgatcgacta 647 24 0 45.83 57.434 12.00 6.00 6.566 747 tagctagctagcatgatcgactag 646 24 0 45.83 57.434 10.00 8.00 6.566 748 tagctagcatgatcgactagctag 642 24 0 45.83 57.434 11.00 8.00 6.566 749 tagcatcgatcagtagctagctag 324 24 0 45.83 57.434 10.00 8.00 6.566 750 gatgtagcatcgatcagtagctag 328 24 0 45.83 57.431 6.00 4.00 6.569 751 cgatgtagcatcgatcagtagctag 329 25 0 48.00 61.570 8.00 4.00 6.570 752 tgtagcatcgatcagtagctagctag 326 26 0 46.15 60.576 10.00 8.00 6.576 753 ctagctagctagcatactagcatcg 356 25 0 48.00 58.419 12.00 8.00 6.581 754 tatcagtcagctagctagtagcatgc 482 26 0 46.15 60.584 8.00 7.00 6.584 755 tagctagctagcatactagcatcgat 355 26 0 42.31 59.390 10.00 8.00 6.610 756 agctactagctagcatcgatcgatc 144 25 0 48.00 61.611 10.00 8.00 6.611 757 tagctagcatactagcatcgatcga 351 25 0 44.00 61.618 10.00 8.00 6.618 758 tgatgatcgatcagtcagctagct 283 24 0 45.83 62.625 12.00 8.00 6.625 759 gatcgatcagtcagctagctaaata 279 25 0 40.00 58.360 8.00 6.00 6.640 760 ctagctagctagcatgatcgactag 647 25 0 48.00 58.347 12.00 8.00 6.653 761 cgcagagatgatagtagctagctag 373 25 0 48.00 58.347 10.00 8.00 6.653 762 tagcatgatcgactagctagctac 638 24 0 45.83 57.298 10.00 8.00 6.702 763 tcgatcagtcagctagctaaatag 277 24 0 41.67 57.276 8.00 6.00 6.724 764 atcgatcagtcagctagctaaatagc 278 26 0 42.31 60.726 8.00 6.00 6.726 765 tgatcgatcagtcagctagctaaata 280 26 0 38.46 60.727 10.00 6.00 6.727 766 atcagtcagctagctagtagcatg 481 24 0 45.83 57.265 8.00 8.00 6.735 767 ctagcatgatcgactagctagctac 639 25 0 48.00 58.222 10.00 8.00 6.778 768 gtagcatcgatcagtagctagctag 325 25 0 48.00 58.222 10.00 8.00 6.778 769 gatcgatcagtcagctagctaaatag 279 26 0 42.31 59.213 8.00 6.00 6.787 770 tagtagctagctagcatcgcctagc 440 25 0 52.00 61.803 10.00 6.00 6.803 771 tcgcgtatcagtcagctagctagta 487 25 0 48.00 61.817 8.00 4.00 6.817 772 agctagctagcatactagcatcgatc 354 26 0 46.15 60.851 10.00 4.00 6.851 773 tagctactagctagcatcgatgatga 546 26 0 42.31 60.857 11.00 6.00 6.857 774 tagctagtagctagcatgatgatcga 299 26 0 42.31 60.857 11.00 4.00 6.857 775 tgcatcgatcgatgatagtagctag 455 25 0 44.00 61.864 12.00 4.00 6.864 776 tagctagcatcgatgatgatcagc 539 24 0 45.83 62.867 8.00 8.00 6.867 777 gctagcatcgatgatgatcagcta 537 24 0 45.83 62.867 8.00 8.00 6.867 778 tagcatcgatgatgatcagctagc 535 24 0 45.83 62.867 8.00 7.00 6.867 779 gcatcgatgatgatcagctagcta 533 24 0 45.83 62.867 8.00 6.00 6.867 780 tagctagcatcgcctagctagcat 433 24 0 50.00 62.904 10.00 7.00 6.904 781 agctagctagcatcgcgtatcagt 499 24 0 50.00 62.905 10.00 1.00 6.905 782 atgatcgatcagtcagctagctaaat 281 26 0 38.46 60.936 10.00 8.00 6.936 783 gcatgcatcgatcgatgatagtag 458 24 0 45.83 62.969 12.00 6.00 6.969 784 catgcatcgatcgatgatagtagc 457 24 0 45.83 62.969 12.00 4.00 6.969 785 gcgtatcagtcagctagctagtag 485 24 0 50.00 57.004 8.00 6.00 6.996 786 cgtatcagtcagctagctagtagc 484 24 0 50.00 57.004 8.00 8.00 6.996 787 ctagctagcatcgcgtatcagtca 497 24 0 50.00 62.998 8.00 1.00 6.998 788 tagctagcatcgcgtatcagtcag 496 24 0 50.00 62.998 6.00 1.00 6.998 789 ctagcatcgcgtatcagtcagcta 493 24 0 50.00 62.998 4.00 3.00 6.998 790 tagcatcgcgtatcagtcagctag 492 24 0 50.00 62.998 4.00 4.00 6.998 791 catcgcgtatcagtcagctagcta 489 24 0 50.00 62.998 8.00 6.00 6.998 792 aaagctactagctagatgatgatcagc 180 27 0 40.74 60.007 9.00 8.00 7.007 793 atcgcgtatcagtcagctagctagt 488 25 0 48.00 62.015 8.00 6.00 7.015 794 tgatagtagctagctagcatcgcct 443 25 0 48.00 62.039 12.00 4.00 7.039 795 agctactagctagcatcgatgatgat 545 26 0 42.31 61.064 9.00 6.00 7.064 796 agctagtagctagcatgatgatcgat 298 26 0 42.31 61.064 9.00 6.00 7.064 797 atgatcagctagctagtagcatcgat 227 26 0 42.31 61.064 8.00 8.00 7.064 798 atgatgatcagctagctagtagcat 230 25 0 40.00 57.922 8.00 8.00 7.078 799 ctactagctagcatcgatgatgatca 543 26 0 42.31 61.079 8.00 7.00 7.079 800 tactagctagcatcgatgatgatcag 542 26 0 42.31 61.079 8.00 6.00 7.079 801 catcgatgatgatcagctagctagta 532 26 0 42.31 61.079 8.00 4.00 7.079 802 tagtagctagcatgatgatcgatcag 295 26 0 42.31 61.079 10.00 8.00 7.079 803 gactagctagctacgatcgatcagc 628 25 0 52.00 62.095 10.00 6.00 7.095 804 aagctactagctagatgatgatcagc 179 26 0 42.31 58.900 9.00 8.00 7.100 805 tagatgatgatcagctagctagtagca 233 27 0 40.74 60.113 11.00 6.00 7.113 806 ctagctagctagcatactagcatcgat 356 27 0 44.44 60.145 12.00 8.00 7.145 807 gatagctactagctagcatcgatgat 548 26 0 42.31 58.799 11.00 8.00 7.201 808 atcgatcgatgatagtagctagctag 452 26 0 42.31 58.797 10.00 8.00 7.203 809 tcgatcagtcagctagctaaatagct 277 26 0 42.31 61.281 8.00 8.00 7.281 810 gctagcatgatcgactagctagctac 640 26 0 50.00 61.306 10.00 8.00 7.306 811 agatgatgatcagctagctagtagc 232 25 0 44.00 57.687 9.00 8.00 7.313 812 agctactagctagatgatgatcagc 178 25 0 44.00 57.687 9.00 8.00 7.313 813 agatgatgatcagctagctagtagcat 232 27 0 40.74 60.327 9.00 8.00 7.327 814 actagctagcatcgatcgatcagtc 140 25 0 48.00 62.355 10.00 6.00 7.355 815 atcgatcagtcagctagctaaatag 278 25 0 40.00 57.634 8.00 6.00 7.366 816 tcgatgtagcatcgatcagtagcta 330 25 0 44.00 62.368 10.00 6.00 7.368 817 ctacgatcgatcagctagctagcat 581 25 0 48.00 62.397 10.00 8.00 7.397 818 atcgcgcagagatgatagtagctag 377 25 0 48.00 62.397 6.00 4.00 7.397 819 gcgcagagatgatagtagctagctag 374 26 0 50.00 61.404 10.00 8.00 7.404 820 gatcgatcagctagtcagctagctag 614 26 0 50.00 61.427 9.00 8.00 7.427 821 atcgatcgatcagtcagctagctag 130 25 0 48.00 62.453 10.00 8.00 7.453 822 ctactagctagcatcgatcgatcagt 142 26 0 46.15 61.522 10.00 6.00 7.522 823 gatgtagcatcgatcagtagctagct 328 26 0 46.15 61.533 8.00 8.00 7.533 824 agtagctagctagcatcgcctagct 439 25 0 52.00 62.538 10.00 8.00 7.538 825 tgatgatcgatcagtcagctagcta 283 25 0 44.00 62.543 12.00 6.00 7.543 826 atcgatgtagcatcgatcagtagct 331 25 0 44.00 62.565 12.00 4.00 7.565 827 tagctactagctagcatcgatcgatc 145 26 0 46.15 61.570 11.00 8.00 7.570 828 gcatcgatgatcgatagctactagc 560 25 0 48.00 62.588 11.00 7.00 7.588 829 gcatcgatcgatgatagtagctagc 454 25 0 48.00 62.588 12.00 6.00 7.588 830 agctagctagtagcatcgatgatcag 221 26 0 46.15 61.633 8.00 6.00 7.633 831 ctagtagcatcgatgatcagctagct 215 26 0 46.15 61.633 10.00 8.00 7.633 832 agtagcatcgatgatcagctagctag 213 26 0 46.15 61.633 10.00 8.00 7.633 833 tatcagtcagctagctagtagcatg 482 25 0 44.00 57.367 8.00 8.00 7.633 834 tgatgatgatagtagatgatgatcagc 245 27 0 37.04 60.705 8.00 7.00 7.705 835 atgatgatcgatcagtcagctagct 284 25 0 44.00 62.743 12.00 8.00 7.743 836 atgtagcatcgatcagtagctagctag 327 27 0 44.44 60.771 10.00 8.00 7.771 837 atagctactagctagcatcgatgat 547 25 0 40.00 57.227 11.00 8.00 7.773 838 agtagctagcatgatgatcgatcagt 294 26 0 42.31 61.773 10.00 8.00 7.773 839 gatgatgatagtagatgatgatcagc 244 26 0 38.46 58.224 7.00 7.00 7.776 840 tcagctagtcagctagctagatcgc 608 25 0 52.00 62.795 9.00 8.00 7.795 841 tcagtcagctagctagtagcatgca 480 25 0 48.00 62.811 8.00 6.00 7.811 842 gctactagctagcatcgatgatgatc 544 26 0 46.15 61.816 8.00 6.00 7.816 843 gctagtagctagcatgatgatcgatc 297 26 0 46.15 61.816 8.00 8.00 7.816 844 tagctagctagcatcgcgtatcagt 500 25 0 48.00 62.822 10.00 1.00 7.822 845 tagctagctagcatactagcatcgatc 355 27 0 44.44 60.834 10.00 7.00 7.834 846 gtatcagtcagctagctagtagcatg 483 26 0 46.15 58.154 8.00 8.00 7.846 847 cgactagctagctacgatcgatcag 629 25 0 52.00 62.876 10.00 6.00 7.876 848 agcatgatcgactagctagctacga 637 25 0 48.00 62.891 10.00 8.00 7.891 849 ctagctagctagcatcgcgtatcag 501 25 0 52.00 62.912 12.00 2.00 7.912 850 atgatcgatcagtcagctagctaaata 281 27 0 37.04 60.916 10.00 6.00 7.916 851 atagtagctagctagcatcgcctagc 441 26 0 50.00 61.939 10.00 6.00 7.939 852 atcgcgtatcagtcagctagctagta 488 26 0 46.15 61.961 8.00 4.00 7.961 853 tgatagtagctagctagcatcgccta 443 26 0 46.15 61.986 12.00 6.00 7.986 854 atgcatcgatcgatgatagtagctag 456 26 0 42.31 62.008 12.00 4.00 8.008 855 catcgatcgatgatagtagctagctag 453 27 0 44.44 61.031 12.00 8.00 8.031 856 gatagctactagctagcatcgatgatg 548 27 0 44.44 61.040 11.00 6.00 8.040 857 atagctactagctagcatcgatgatga 547 27 0 40.74 61.040 11.00 6.00 8.040 858 tagctactagctagcatcgatgatgat 546 27 0 40.74 61.040 11.00 6.00 8.040 859 tagctagtagctagcatgatgatcgat 299 27 0 40.74 61.040 11.00 6.00 8.040 860 ctagcatgatcgactagctagctacg 639 26 0 50.00 62.056 10.00 8.00 8.056 861 ctagctacgatcgatcagctagtcag 622 26 0 50.00 62.099 12.00 8.00 8.099 862 ctacgatcgatcagctagtcagctag 618 26 0 50.00 62.099 8.00 5.00 8.099 863 atgatagtagctagctagcatcgcct 444 26 0 46.15 62.168 12.00 6.00 8.168 864 tagatgatgatcagctagctagtagc 233 26 0 42.31 57.771 11.00 8.00 8.229 865 gtatcagtcagctagctagtagcatgc 483 27 0 48.15 61.231 8.00 7.00 8.231 866 gatgatcgatcagtcagctagctaaa 282 26 0 42.31 62.274 10.00 6.00 8.274 867 gcataaagctactagctagatgatga 184 26 0 38.46 57.716 11.00 5.00 8.284 868 tactagctagcatcgatcgatcagtc 141 26 0 46.15 62.288 10.00 6.00 8.288 869 cgatagctactagctagcatcgatga 549 26 0 46.15 62.331 12.00 7.00 8.331 870 ctagctagcatactagcatcgatcga 352 26 0 46.15 62.331 10.00 8.00 8.331 871 tcgatgatgatcagctagctagtagc 530 26 0 46.15 62.397 8.00 8.00 8.397 872 gatgatcagctagctagtagcatcga 228 26 0 46.15 62.397 10.00 8.00 8.397 873 tcagctagctagtagcatcgatgatc 223 26 0 46.15 62.397 9.00 6.00 8.397 874 atcgatcagtcagctagctaaatagct 278 27 0 40.74 61.441 8.00 8.00 8.441 875 agcataaagctactagctagatgatga 185 27 0 37.04 58.559 11.00 6.00 8.441 876 tgatcgatcagtcagctagctaaatag 280 27 0 40.74 61.469 10.00 6.00 8.469 877 tagtagctagctagcatcgcctagct 440 26 0 50.00 62.471 10.00 8.00 8.471 878 gtagatgatgatcagctagctagtagc 234 27 0 44.44 58.516 11.00 8.00 8.484 879 atcgatgtagcatcgatcagtagcta 331 26 0 42.31 62.492 12.00 6.00 8.492 880 tcgcgtatcagtcagctagctagtag 487 26 0 50.00 62.530 8.00 6.00 8.530 881 gcgtatcagtcagctagctagtagca 485 26 0 50.00 62.543 8.00 6.00 8.543 882 gatgatgatgatagtagatgatgatca 247 27 0 33.33 58.451 8.00 8.00 8.549 883 tagtagcatcgatgatcagctagctag 214 27 0 44.44 61.591 10.00 8.00 8.591 884 atgatgatcgatcagtcagctagcta 284 26 0 42.31 62.662 12.00 6.00 8.662 885 tgatgatgatagtagatgatgatcag 245 26 0 34.62 57.322 8.00 5.00 8.678 886 tagtagctagcatgatgatcgatcagt 295 27 0 40.74 61.726 10.00 8.00 8.726 887 gactagctagctacgatcgatcagct 628 26 0 50.00 62.792 10.00 8.00 8.792 888 tagcatgatcgactagctagctacga 638 26 0 46.15 62.809 10.00 8.00 8.809 889 ctactagctagcatcgatgatgatcag 543 27 0 44.44 61.823 8.00 6.00 8.823 890 catcgatgatgatcagctagctagtag 532 27 0 44.44 61.823 8.00 6.00 8.823 891 ctagtagctagcatgatgatcgatcag 296 27 0 44.44 61.823 10.00 8.00 8.823 892 atcagctagtcagctagctagatcgc 609 26 0 50.00 62.896 11.00 8.00 8.896 893 atcagtcagctagctagtagcatgca 481 26 0 46.15 62.915 8.00 6.00 8.915 894 tcagtcagctagctagtagcatgcat 480 26 0 46.15 62.915 8.00 6.00 8.915 895 gcataaagctactagctagatgatgat 184 27 0 37.04 58.031 11.00 4.00 8.969 896 agcataaagctactagctagatgatg 185 26 0 38.46 57.029 11.00 7.00 8.971 897 cgtatcagtcagctagctagtagcatg 484 27 0 48.15 61.978 8.00 8.00 8.978 898 agctagcatgatcgactagctagctac 641 27 0 48.15 62.004 12.00 8.00 9.004 899 atgatagtagctagctagcatcgccta 444 27 0 44.44 62.113 12.00 6.00 9.113 900 gatcgatcagtcagctagctaaatagc 279 27 0 44.44 62.155 8.00 6.00 9.155 901 atgatgatgatagtagatgatgatcag 246 27 0 33.33 57.694 8.00 6.00 9.306 902 gatgatcgatcagtcagctagctaaat 282 27 0 40.74 62.397 10.00 8.00 9.397 903 cgatagctactagctagcatcgatgat 549 27 0 44.44 62.447 12.00 8.00 9.447 904 agctactagctagcatcgatgatgatc 545 27 0 44.44 62.514 9.00 6.00 9.514 905 atcgatgatgatcagctagctagtagc 531 27 0 44.44 62.514 8.00 8.00 9.514 906 agctagtagctagcatgatgatcgatc 298 27 0 44.44 62.514 9.00 8.00 9.514 907 atcagctagctagtagcatcgatgatc 224 27 0 44.44 62.514 11.00 7.00 9.514 908 atagtagctagctagcatcgcctagct 441 27 0 48.15 62.576 10.00 8.00 9.576 909 atcgcgtatcagtcagctagctagtag 488 27 0 48.15 62.638 8.00 6.00 9.638 910 tgatagtagctagctagcatcgcctag 443 27 0 48.15 62.644 12.00 4.00 9.644 911 gcgtatcagtcagctagctagtagcat 485 27 0 48.15 62.652 8.00 6.00 9.652 912 tatcagtcagctagctagtagcatgca 482 27 0 44.44 62.833 8.00 6.00 9.833 913 ctactagctagcatcgatcgatcagtc 142 27 0 48.15 62.974 10.00 6.00 9.974 914 taaagctactagctagatgatgatcag 181 27 0 37.04 57.019 9.00 6.00 9.981 primer3-2.3.6/test/primer1_list_last/SP01-02.txt.rev0000664000077300007730000000375112231156243020734 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TCCTGTTGGAGAAAAGCCAC 248 20 0 50.00 60.232 3.00 0.00 0.232 1 CCTGTTGGAGAAAAGCCACT 247 20 0 50.00 59.328 3.00 1.00 0.672 2 TTCCTGTTGGAGAAAAGCCA 249 20 0 45.00 60.745 3.00 0.00 0.745 3 TGTTGGAGAAAAGCCACTGG 245 20 0 50.00 61.204 5.00 5.00 1.204 4 CTGTTGGAGAAAAGCCACTG 246 20 0 50.00 58.496 3.00 3.00 1.504 5 TCCTGTTGGAGAAAAGCCA 248 19 0 47.37 59.354 3.00 0.00 1.646 6 TGGAGAAAAGCCACTGGC 242 18 0 55.56 59.918 7.00 7.00 2.082 7 GGAGAAAAGCCACTGGCA 241 18 0 55.56 59.918 7.00 5.00 2.082 8 GTTGGAGAAAAGCCACTGGC 244 20 0 55.00 62.088 7.00 7.00 2.088 9 TCCTGTTGGAGAAAAGCCACT 248 21 0 47.62 61.165 3.00 1.00 2.165 10 TTGGAGAAAAGCCACTGGC 243 19 0 52.63 61.313 7.00 7.00 2.313 11 TTCCTGTTGGAGAAAAGCCAC 249 21 0 47.62 61.523 3.00 0.00 2.523 12 CCTGTTGGAGAAAAGCCAC 247 19 0 52.63 58.272 3.00 0.00 2.728 13 GTTGGAGAAAAGCCACTGG 244 19 0 52.63 58.272 5.00 5.00 2.728 14 CCTGTTGGAGAAAAGCCACTG 247 21 0 52.38 62.093 3.00 3.00 3.093 15 CTGTTGGAGAAAAGCCACTGG 246 21 0 52.38 62.093 5.00 5.00 3.093 16 TTCCTGTTGGAGAAAAGCC 249 19 0 47.37 57.850 3.00 1.00 3.150 17 TGTTGGAGAAAAGCCACTG 245 19 0 47.37 57.360 3.00 3.00 3.640 18 TGGAGAAAAGCCACTGGCA 242 19 0 52.63 62.902 7.00 5.00 3.902 19 TTCCTGTTGGAGAAAAGCCACT 249 22 0 45.45 62.351 3.00 1.00 4.351 20 CCTGTTGGAGAAAAGCCA 247 18 0 50.00 57.278 3.00 0.00 4.722 21 TTGGAGAAAAGCCACTGG 243 18 0 50.00 57.278 5.00 5.00 4.722 22 GACCCCACGCACGCTAAG 159 18 0 66.67 62.798 3.00 3.00 4.798 primer3-2.3.6/test/primer1_list_last/SP01-19.txt.for0000664000077300007730000001753012231156243020736 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TATTCCTGTCCCTGTGCCTC 49 20 0 55.00 60.073 2.00 1.00 0.073 1 GCCTCAGAAACCTGGCTACA 64 20 0 55.00 60.397 3.00 1.00 0.397 2 TGCCTCAGAAACCTGGCTAC 63 20 0 55.00 60.397 3.00 3.00 0.397 3 GTGCCTCAGAAACCTGGCTA 62 20 0 55.00 60.397 3.00 2.00 0.397 4 CTGTCCCTGTGCCTCAGAA 54 19 0 57.89 59.963 5.00 1.00 1.037 5 ATTCCTGTCCCTGTGCCTC 50 19 0 57.89 60.064 2.00 1.00 1.064 6 AATATTCCTGTCCCTGTGCC 47 20 0 50.00 58.864 6.00 1.00 1.136 7 AATATTCCTGTCCCTGTGCCT 47 21 0 47.62 59.841 6.00 0.00 1.159 8 CCCTGTGCCTCAGAAACCT 58 19 0 57.89 60.245 5.00 0.00 1.245 9 TGTCCCTGTGCCTCAGAAAC 55 20 0 55.00 61.259 5.00 5.00 1.259 10 ATATTCCTGTCCCTGTGCCTC 48 21 0 52.38 60.342 4.00 1.00 1.342 11 GAAACCTGGCTACAGTTCCC 70 20 0 55.00 58.646 3.00 3.00 1.354 12 AAACCTGGCTACAGTTCCCTC 71 21 0 52.38 59.630 3.00 0.00 1.370 13 GAAACCTGGCTACAGTTCCCT 70 21 0 52.38 59.630 3.00 1.00 1.370 14 AGAAACCTGGCTACAGTTCCC 69 21 0 52.38 59.630 3.00 3.00 1.370 15 TGTCCCTGTGCCTCAGAAA 55 19 0 52.63 60.385 5.00 3.00 1.385 16 CTGTCCCTGTGCCTCAGAAA 54 20 0 55.00 61.390 5.00 3.00 1.390 17 GTGCCTCAGAAACCTGGCT 62 19 0 57.89 60.402 3.00 3.00 1.402 18 TGCCTCAGAAACCTGGCTA 63 19 0 52.63 59.531 3.00 2.00 1.469 19 ATATTCCTGTCCCTGTGCCT 48 20 0 50.00 58.473 4.00 0.00 1.527 20 CTGTGCCTCAGAAACCTGG 60 19 0 57.89 59.399 5.00 3.00 1.601 21 CCTGTGCCTCAGAAACCTG 59 19 0 57.89 59.399 5.00 2.00 1.601 22 AACCTGGCTACAGTTCCCTC 72 20 0 55.00 58.255 3.00 0.00 1.745 23 GTCCCTGTGCCTCAGAAACC 56 20 0 60.00 62.024 5.00 3.00 2.024 24 TAATATTCCTGTCCCTGTGCC 46 21 0 47.62 58.917 6.00 1.00 2.083 25 TAATATTCCTGTCCCTGTGCCT 46 22 0 45.45 59.856 6.00 0.00 2.144 26 TCCCTGTGCCTCAGAAACCT 57 20 0 55.00 62.145 5.00 0.00 2.145 27 CAGAAACCTGGCTACAGTTCC 68 21 0 52.38 58.851 3.00 2.00 2.149 28 CCTCAGAAACCTGGCTACAGTT 65 22 0 50.00 59.810 3.00 2.00 2.190 29 GTGCCTCAGAAACCTGGCTAC 62 21 0 57.14 61.195 3.00 3.00 2.195 30 TTAATATTCCTGTCCCTGTGCC 45 22 0 45.45 60.204 6.00 1.00 2.204 31 AAACCTGGCTACAGTTCCCT 71 20 0 50.00 57.789 3.00 1.00 2.211 32 TCCCTGTGCCTCAGAAACC 57 19 0 57.89 61.213 5.00 1.00 2.213 33 TTCCTGTCCCTGTGCCTC 51 18 0 61.11 59.749 2.00 1.00 2.251 34 GCCTCAGAAACCTGGCTACAG 64 21 0 57.14 61.316 3.00 3.00 2.316 35 CCTGTCCCTGTGCCTCAG 53 18 0 66.67 60.405 5.00 5.00 2.405 36 CCTCAGAAACCTGGCTACAG 65 20 0 55.00 57.547 3.00 3.00 2.453 37 TGCCTCAGAAACCTGGCT 63 18 0 55.56 59.499 3.00 3.00 2.501 38 AGAAACCTGGCTACAGTTCCCT 69 22 0 50.00 60.528 3.00 2.00 2.528 39 CCTCAGAAACCTGGCTACAGT 65 21 0 52.38 58.468 3.00 3.00 2.532 40 GTGCCTCAGAAACCTGGC 62 18 0 61.11 59.345 3.00 2.00 2.655 41 TCAGAAACCTGGCTACAGTTCC 67 22 0 50.00 60.661 3.00 2.00 2.661 42 GTCCCTGTGCCTCAGAAAC 56 19 0 57.89 58.201 5.00 5.00 2.799 43 CCCTGTGCCTCAGAAACC 58 18 0 61.11 59.180 5.00 1.00 2.820 44 TATTCCTGTCCCTGTGCCT 49 19 0 52.63 58.086 2.00 0.00 2.914 45 ATTCCTGTCCCTGTGCCTCA 50 20 0 55.00 62.996 3.00 3.00 2.996 46 CTGTCCCTGTGCCTCAGAAAC 54 21 0 57.14 62.180 5.00 5.00 3.180 47 TGTCCCTGTGCCTCAGAA 55 18 0 55.56 58.812 5.00 1.00 3.188 48 GAAACCTGGCTACAGTTCCCTC 70 22 0 54.55 61.364 3.00 0.00 3.364 49 TGTGCCTCAGAAACCTGGC 61 19 0 57.89 62.397 3.00 2.00 3.397 50 ATTAATATTCCTGTCCCTGTGCC 44 23 0 43.48 60.438 6.00 1.00 3.438 51 TCCTGTCCCTGTGCCTCA 52 18 0 61.11 61.468 3.00 3.00 3.468 52 CCTGTCCCTGTGCCTCAGA 53 19 0 63.16 62.470 5.00 3.00 3.470 53 TCCTGTCCCTGTGCCTCAG 52 19 0 63.16 62.470 5.00 5.00 3.470 54 GCCTCAGAAACCTGGCTAC 64 19 0 57.89 57.465 3.00 3.00 3.535 55 AATATTCCTGTCCCTGTGCCTC 47 22 0 50.00 61.574 6.00 1.00 3.574 56 ATATTCCTGTCCCTGTGCC 48 19 0 52.63 57.370 4.00 1.00 3.630 57 CTGTCCCTGTGCCTCAGA 54 18 0 61.11 58.348 5.00 3.00 3.652 58 TGTGCCTCAGAAACCTGG 61 18 0 55.56 58.249 3.00 3.00 3.751 59 CTCAGAAACCTGGCTACAGTTC 66 22 0 50.00 58.135 3.00 2.00 3.865 60 TTCCTGTCCCTGTGCCTCA 51 19 0 57.89 62.869 3.00 3.00 3.869 61 GTCCCTGTGCCTCAGAAACCT 56 21 0 57.14 62.881 5.00 0.00 3.881 62 TATTCCTGTCCCTGTGCCTCA 49 21 0 52.38 62.891 3.00 3.00 3.891 63 TCAGAAACCTGGCTACAGTTC 67 21 0 47.62 57.065 3.00 2.00 3.935 64 ATTCCTGTCCCTGTGCCT 50 18 0 55.56 57.991 2.00 0.00 4.009 65 TTAATATTCCTGTCCCTGTGCCT 45 23 0 43.48 61.044 6.00 0.00 4.044 66 GCCTCAGAAACCTGGCTACAGT 64 22 0 54.55 62.045 3.00 3.00 4.045 67 CAGAAACCTGGCTACAGTTCCC 68 22 0 54.55 62.245 3.00 3.00 4.245 68 TATTAATATTCCTGTCCCTGTGCC 43 24 0 41.67 60.432 8.00 1.00 4.432 69 CTCAGAAACCTGGCTACAGTTCC 66 23 0 52.17 61.514 3.00 2.00 4.514 70 CCTCAGAAACCTGGCTACAGTTC 65 23 0 52.17 61.514 3.00 2.00 4.514 71 TAATATTCCTGTCCCTGTGCCTC 46 23 0 47.83 61.529 6.00 1.00 4.529 72 TCCCTGTGCCTCAGAAAC 57 18 0 55.56 57.158 5.00 3.00 4.842 73 GTCCCTGTGCCTCAGAAA 56 18 0 55.56 57.158 5.00 3.00 4.842 74 ATTAATATTCCTGTCCCTGTGC 44 22 0 40.91 57.155 6.00 2.00 4.845 75 AGAAACCTGGCTACAGTTCCCTC 69 23 0 52.17 62.164 3.00 0.00 5.164 76 ATTAATATTCCTGTCCCTGTGCCT 44 24 0 41.67 61.230 6.00 0.00 5.230 77 ATATTAATATTCCTGTCCCTGTGCC 42 25 0 40.00 60.638 10.00 1.00 5.638 78 TATTAATATTCCTGTCCCTGTGC 43 23 0 39.13 57.263 8.00 2.00 5.737 79 AAATATTAATATTCCTGTCCCTGTGC 40 26 0 34.62 59.917 12.00 2.00 6.083 80 AATATTAATATTCCTGTCCCTGTGC 41 25 0 36.00 58.823 12.00 2.00 6.177 81 TATTAATATTCCTGTCCCTGTGCCT 43 25 0 40.00 61.200 8.00 0.00 6.200 82 ATATTAATATTCCTGTCCCTGTGC 42 24 0 37.50 57.627 10.00 2.00 6.373 83 TTAATATTCCTGTCCCTGTGCCTC 45 24 0 45.83 62.607 6.00 1.00 6.607 84 CAAATATTAATATTCCTGTCCCTGTG 39 26 0 34.62 59.176 12.00 1.00 6.824 85 TCAAATATTAATATTCCTGTCCCTG 38 25 0 32.00 57.690 12.00 1.00 7.310 86 ATATTAATATTCCTGTCCCTGTGCCT 42 26 0 38.46 61.365 10.00 0.00 7.365 87 CTCAAATATTAATATTCCTGTCCCTG 37 26 0 34.62 58.540 12.00 1.00 7.460 88 TCAAATATTAATATTCCTGTCCCTGT 38 26 0 30.77 58.425 12.00 0.00 7.575 89 TCAAATATTAATATTCCTGTCCCTGTG 38 27 0 33.33 60.641 12.00 1.00 7.641 90 AATATTAATATTCCTGTCCCTGTGCC 41 26 0 38.46 61.650 12.00 1.00 7.650 91 ATTAATATTCCTGTCCCTGTGCCTC 44 25 0 44.00 62.718 6.00 1.00 7.718 92 CTCAAATATTAATATTCCTGTCCCTGT 37 27 0 33.33 59.222 12.00 0.00 7.778 93 TATTAATATTCCTGTCCCTGTGCCTC 43 26 0 42.31 62.643 8.00 1.00 8.643 94 CAAATATTAATATTCCTGTCCCTGTGC 39 27 0 37.04 62.049 12.00 2.00 9.049 95 AATATTAATATTCCTGTCCCTGTGCCT 41 27 0 37.04 62.311 12.00 0.00 9.311 96 AAATATTAATATTCCTGTCCCTGTGCC 40 27 0 37.04 62.579 12.00 1.00 9.579 97 ATATTAATATTCCTGTCCCTGTGCCTC 42 27 0 40.74 62.745 10.00 1.00 9.745 primer3-2.3.6/test/primer1_list_last/AGAG.rev0000664000077300007730000001537112231156243017714 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TCTCTCTCTCTCTCTCTCTCTCTCTC 155 26 0 50.00 57.752 0.00 0.00 8.248 1 TCTCTCTCTCTCTCTCTCTCTCTCTC 153 26 0 50.00 57.752 0.00 0.00 8.248 2 TCTCTCTCTCTCTCTCTCTCTCTCTC 151 26 0 50.00 57.752 0.00 0.00 8.248 3 TCTCTCTCTCTCTCTCTCTCTCTCTC 149 26 0 50.00 57.752 0.00 0.00 8.248 4 TCTCTCTCTCTCTCTCTCTCTCTCTC 147 26 0 50.00 57.752 0.00 0.00 8.248 5 TCTCTCTCTCTCTCTCTCTCTCTCTC 145 26 0 50.00 57.752 0.00 0.00 8.248 6 TCTCTCTCTCTCTCTCTCTCTCTCTC 143 26 0 50.00 57.752 0.00 0.00 8.248 7 TCTCTCTCTCTCTCTCTCTCTCTCTC 141 26 0 50.00 57.752 0.00 0.00 8.248 8 TCTCTCTCTCTCTCTCTCTCTCTCTC 139 26 0 50.00 57.752 0.00 0.00 8.248 9 TCTCTCTCTCTCTCTCTCTCTCTCTC 137 26 0 50.00 57.752 0.00 0.00 8.248 10 TCTCTCTCTCTCTCTCTCTCTCTCTC 135 26 0 50.00 57.752 0.00 0.00 8.248 11 TCTCTCTCTCTCTCTCTCTCTCTCTC 133 26 0 50.00 57.752 0.00 0.00 8.248 12 TCTCTCTCTCTCTCTCTCTCTCTCTC 131 26 0 50.00 57.752 0.00 0.00 8.248 13 TCTCTCTCTCTCTCTCTCTCTCTCTC 129 26 0 50.00 57.752 0.00 0.00 8.248 14 TCTCTCTCTCTCTCTCTCTCTCTCTC 127 26 0 50.00 57.752 0.00 0.00 8.248 15 TCTCTCTCTCTCTCTCTCTCTCTCTC 125 26 0 50.00 57.752 0.00 0.00 8.248 16 TCTCTCTCTCTCTCTCTCTCTCTCTC 123 26 0 50.00 57.752 0.00 0.00 8.248 17 TCTCTCTCTCTCTCTCTCTCTCTCTC 121 26 0 50.00 57.752 0.00 0.00 8.248 18 TCTCTCTCTCTCTCTCTCTCTCTCTC 119 26 0 50.00 57.752 0.00 0.00 8.248 19 TCTCTCTCTCTCTCTCTCTCTCTCTC 117 26 0 50.00 57.752 0.00 0.00 8.248 20 TCTCTCTCTCTCTCTCTCTCTCTCTC 115 26 0 50.00 57.752 0.00 0.00 8.248 21 TCTCTCTCTCTCTCTCTCTCTCTCTC 113 26 0 50.00 57.752 0.00 0.00 8.248 22 TCTCTCTCTCTCTCTCTCTCTCTCTC 111 26 0 50.00 57.752 0.00 0.00 8.248 23 TCTCTCTCTCTCTCTCTCTCTCTCTC 109 26 0 50.00 57.752 0.00 0.00 8.248 24 TCTCTCTCTCTCTCTCTCTCTCTCTC 107 26 0 50.00 57.752 0.00 0.00 8.248 25 TCTCTCTCTCTCTCTCTCTCTCTCTC 105 26 0 50.00 57.752 0.00 0.00 8.248 26 TCTCTCTCTCTCTCTCTCTCTCTCTC 103 26 0 50.00 57.752 0.00 0.00 8.248 27 TCTCTCTCTCTCTCTCTCTCTCTCTC 101 26 0 50.00 57.752 0.00 0.00 8.248 28 TCTCTCTCTCTCTCTCTCTCTCTCTCT 155 27 0 48.15 58.701 0.00 0.00 8.299 29 CTCTCTCTCTCTCTCTCTCTCTCTCTC 154 27 0 51.85 58.701 0.00 0.00 8.299 30 TCTCTCTCTCTCTCTCTCTCTCTCTCT 153 27 0 48.15 58.701 0.00 0.00 8.299 31 CTCTCTCTCTCTCTCTCTCTCTCTCTC 152 27 0 51.85 58.701 0.00 0.00 8.299 32 TCTCTCTCTCTCTCTCTCTCTCTCTCT 151 27 0 48.15 58.701 0.00 0.00 8.299 33 CTCTCTCTCTCTCTCTCTCTCTCTCTC 150 27 0 51.85 58.701 0.00 0.00 8.299 34 TCTCTCTCTCTCTCTCTCTCTCTCTCT 149 27 0 48.15 58.701 0.00 0.00 8.299 35 CTCTCTCTCTCTCTCTCTCTCTCTCTC 148 27 0 51.85 58.701 0.00 0.00 8.299 36 TCTCTCTCTCTCTCTCTCTCTCTCTCT 147 27 0 48.15 58.701 0.00 0.00 8.299 37 CTCTCTCTCTCTCTCTCTCTCTCTCTC 146 27 0 51.85 58.701 0.00 0.00 8.299 38 TCTCTCTCTCTCTCTCTCTCTCTCTCT 145 27 0 48.15 58.701 0.00 0.00 8.299 39 CTCTCTCTCTCTCTCTCTCTCTCTCTC 144 27 0 51.85 58.701 0.00 0.00 8.299 40 TCTCTCTCTCTCTCTCTCTCTCTCTCT 143 27 0 48.15 58.701 0.00 0.00 8.299 41 CTCTCTCTCTCTCTCTCTCTCTCTCTC 142 27 0 51.85 58.701 0.00 0.00 8.299 42 TCTCTCTCTCTCTCTCTCTCTCTCTCT 141 27 0 48.15 58.701 0.00 0.00 8.299 43 CTCTCTCTCTCTCTCTCTCTCTCTCTC 140 27 0 51.85 58.701 0.00 0.00 8.299 44 TCTCTCTCTCTCTCTCTCTCTCTCTCT 139 27 0 48.15 58.701 0.00 0.00 8.299 45 CTCTCTCTCTCTCTCTCTCTCTCTCTC 138 27 0 51.85 58.701 0.00 0.00 8.299 46 TCTCTCTCTCTCTCTCTCTCTCTCTCT 137 27 0 48.15 58.701 0.00 0.00 8.299 47 CTCTCTCTCTCTCTCTCTCTCTCTCTC 136 27 0 51.85 58.701 0.00 0.00 8.299 48 TCTCTCTCTCTCTCTCTCTCTCTCTCT 135 27 0 48.15 58.701 0.00 0.00 8.299 49 CTCTCTCTCTCTCTCTCTCTCTCTCTC 134 27 0 51.85 58.701 0.00 0.00 8.299 50 TCTCTCTCTCTCTCTCTCTCTCTCTCT 133 27 0 48.15 58.701 0.00 0.00 8.299 51 CTCTCTCTCTCTCTCTCTCTCTCTCTC 132 27 0 51.85 58.701 0.00 0.00 8.299 52 TCTCTCTCTCTCTCTCTCTCTCTCTCT 131 27 0 48.15 58.701 0.00 0.00 8.299 53 CTCTCTCTCTCTCTCTCTCTCTCTCTC 130 27 0 51.85 58.701 0.00 0.00 8.299 54 TCTCTCTCTCTCTCTCTCTCTCTCTCT 129 27 0 48.15 58.701 0.00 0.00 8.299 55 CTCTCTCTCTCTCTCTCTCTCTCTCTC 128 27 0 51.85 58.701 0.00 0.00 8.299 56 TCTCTCTCTCTCTCTCTCTCTCTCTCT 127 27 0 48.15 58.701 0.00 0.00 8.299 57 CTCTCTCTCTCTCTCTCTCTCTCTCTC 126 27 0 51.85 58.701 0.00 0.00 8.299 58 TCTCTCTCTCTCTCTCTCTCTCTCTCT 125 27 0 48.15 58.701 0.00 0.00 8.299 59 CTCTCTCTCTCTCTCTCTCTCTCTCTC 124 27 0 51.85 58.701 0.00 0.00 8.299 60 TCTCTCTCTCTCTCTCTCTCTCTCTCT 123 27 0 48.15 58.701 0.00 0.00 8.299 61 CTCTCTCTCTCTCTCTCTCTCTCTCTC 122 27 0 51.85 58.701 0.00 0.00 8.299 62 TCTCTCTCTCTCTCTCTCTCTCTCTCT 121 27 0 48.15 58.701 0.00 0.00 8.299 63 CTCTCTCTCTCTCTCTCTCTCTCTCTC 120 27 0 51.85 58.701 0.00 0.00 8.299 64 TCTCTCTCTCTCTCTCTCTCTCTCTCT 119 27 0 48.15 58.701 0.00 0.00 8.299 65 CTCTCTCTCTCTCTCTCTCTCTCTCTC 118 27 0 51.85 58.701 0.00 0.00 8.299 66 TCTCTCTCTCTCTCTCTCTCTCTCTCT 117 27 0 48.15 58.701 0.00 0.00 8.299 67 CTCTCTCTCTCTCTCTCTCTCTCTCTC 116 27 0 51.85 58.701 0.00 0.00 8.299 68 TCTCTCTCTCTCTCTCTCTCTCTCTCT 115 27 0 48.15 58.701 0.00 0.00 8.299 69 CTCTCTCTCTCTCTCTCTCTCTCTCTC 114 27 0 51.85 58.701 0.00 0.00 8.299 70 TCTCTCTCTCTCTCTCTCTCTCTCTCT 113 27 0 48.15 58.701 0.00 0.00 8.299 71 CTCTCTCTCTCTCTCTCTCTCTCTCTC 112 27 0 51.85 58.701 0.00 0.00 8.299 72 TCTCTCTCTCTCTCTCTCTCTCTCTCT 111 27 0 48.15 58.701 0.00 0.00 8.299 73 CTCTCTCTCTCTCTCTCTCTCTCTCTC 110 27 0 51.85 58.701 0.00 0.00 8.299 74 TCTCTCTCTCTCTCTCTCTCTCTCTCT 109 27 0 48.15 58.701 0.00 0.00 8.299 75 CTCTCTCTCTCTCTCTCTCTCTCTCTC 108 27 0 51.85 58.701 0.00 0.00 8.299 76 TCTCTCTCTCTCTCTCTCTCTCTCTCT 107 27 0 48.15 58.701 0.00 0.00 8.299 77 CTCTCTCTCTCTCTCTCTCTCTCTCTC 106 27 0 51.85 58.701 0.00 0.00 8.299 78 TCTCTCTCTCTCTCTCTCTCTCTCTCT 105 27 0 48.15 58.701 0.00 0.00 8.299 79 CTCTCTCTCTCTCTCTCTCTCTCTCTC 104 27 0 51.85 58.701 0.00 0.00 8.299 80 TCTCTCTCTCTCTCTCTCTCTCTCTCT 103 27 0 48.15 58.701 0.00 0.00 8.299 81 CTCTCTCTCTCTCTCTCTCTCTCTCTC 102 27 0 51.85 58.701 0.00 0.00 8.299 82 TCTCTCTCTCTCTCTCTCTCTCTCTCT 101 27 0 48.15 58.701 0.00 0.00 8.299 83 CTCTCTCTCTCTCTCTCTCTCTCTCTC 100 27 0 51.85 58.701 0.00 0.00 8.299 primer3-2.3.6/test/primer1_list_last/SP01-06.txt.rev0000664000077300007730000001703112231156243020734 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 GTGCTTTGCGTTTTGGAAGT 126 20 0 45.00 60.292 3.00 1.00 0.292 1 TGCTTTGCGTTTTGGAAGTT 125 20 0 40.00 60.782 3.00 3.00 0.782 2 GTGCTTTGCGTTTTGGAAG 126 19 0 47.37 59.454 3.00 0.00 1.546 3 TGCTTTGCGTTTTGGAAGT 125 19 0 42.11 59.454 3.00 1.00 1.546 4 TGCTTTGCGTTTTGGAAGTTA 125 21 0 38.10 60.764 3.00 2.00 1.764 5 GCTTTGCGTTTTGGAAGTTA 124 20 0 40.00 58.097 3.00 2.00 1.903 6 GCTTTGCGTTTTGGAAGTTAG 124 21 0 42.86 59.065 3.00 0.00 1.935 7 GAGCGTGCTTTGCGTTTT 130 18 0 50.00 60.142 4.00 0.00 2.142 8 GTGCTTTGCGTTTTGGAAGTT 126 21 0 42.86 61.528 3.00 3.00 2.528 9 GCTTTGCGTTTTGGAAGTTAGA 124 22 0 40.91 60.748 3.00 1.00 2.748 10 GCTTTGCGTTTTGGAAGTT 124 19 0 42.11 58.013 3.00 3.00 2.987 11 AGCGTGCTTTGCGTTTTG 129 18 0 50.00 61.131 4.00 0.00 3.131 12 GCGTTTTGGAAGTTAGACTTTGA 119 23 0 39.13 59.821 6.00 4.00 3.179 13 TGCTTTGCGTTTTGGAAG 125 18 0 44.44 58.546 3.00 0.00 3.454 14 GTGCTTTGCGTTTTGGAAGTTA 126 22 0 40.91 61.484 3.00 2.00 3.484 15 TGCTTTGCGTTTTGGAAGTTAG 125 22 0 40.91 61.593 3.00 0.00 3.593 16 GTGCTTTGCGTTTTGGAA 126 18 0 44.44 58.389 2.00 2.00 3.611 17 TTTGCGTTTTGGAAGTTAGACTT 122 23 0 34.78 59.378 6.00 6.00 3.622 18 TTGCGTTTTGGAAGTTAGACTTT 121 23 0 34.78 59.378 6.00 4.00 3.622 19 TGCGTTTTGGAAGTTAGACTTTG 120 23 0 39.13 60.658 6.00 2.00 3.658 20 CTTTGCGTTTTGGAAGTTAGA 123 21 0 38.10 57.272 3.00 1.00 3.728 21 CTTTGCGTTTTGGAAGTTAGAC 123 22 0 40.91 58.127 3.00 2.00 3.873 22 TTTGCGTTTTGGAAGTTAGACT 122 22 0 36.36 58.127 4.00 4.00 3.873 23 TTGCGTTTTGGAAGTTAGACTT 121 22 0 36.36 58.127 6.00 6.00 3.873 24 TGCGTTTTGGAAGTTAGACTTT 120 22 0 36.36 58.127 6.00 4.00 3.873 25 GCGTTTTGGAAGTTAGACTTTG 119 22 0 40.91 58.127 6.00 2.00 3.873 26 TTTGCGTTTTGGAAGTTAGAC 122 21 0 38.10 57.124 3.00 2.00 3.876 27 CGTGCTTTGCGTTTTGGA 127 18 0 50.00 61.944 2.00 0.00 3.944 28 CTTTGCGTTTTGGAAGTTAGACT 123 23 0 39.13 59.045 4.00 4.00 3.955 29 GAGCGTGCTTTGCGTTTTG 130 19 0 52.63 62.977 4.00 0.00 3.977 30 CTTTGCGTTTTGGAAGTTAGACTT 123 24 0 37.50 60.206 6.00 6.00 4.206 31 CGTTTTGGAAGTTAGACTTTGACA 118 24 0 37.50 59.738 6.00 4.00 4.262 32 GCTTTGCGTTTTGGAAGTTAGAC 124 23 0 43.48 61.438 3.00 2.00 4.438 33 GGAGCGTGCTTTGCGTTT 131 18 0 55.56 62.465 4.00 0.00 4.465 34 TTTGCGTTTTGGAAGTTAGACTTT 122 24 0 33.33 60.517 6.00 4.00 4.517 35 GCGTTTTGGAAGTTAGACTTTGAC 119 24 0 41.67 60.537 6.00 3.00 4.537 36 GATCGGGAGCGTGCTTTG 136 18 0 61.11 62.907 4.00 0.00 4.907 37 GTGCTTTGCGTTTTGGAAGTTAG 126 23 0 43.48 62.256 3.00 0.00 5.256 38 TTGGAAGTTAGACTTTGACAACAGA 114 25 0 36.00 59.394 6.00 3.00 5.606 39 TTTTGGAAGTTAGACTTTGACAACA 116 25 0 32.00 59.292 6.00 4.00 5.708 40 TTGCGTTTTGGAAGTTAGACTTTG 121 24 0 37.50 61.761 6.00 2.00 5.761 41 CGTTTTGGAAGTTAGACTTTGAC 118 23 0 39.13 57.204 6.00 3.00 5.796 42 CGTTTTGGAAGTTAGACTTTGACAA 118 25 0 36.00 60.875 6.00 6.00 5.875 43 TGGAAGTTAGACTTTGACAACAGA 113 24 0 37.50 58.112 6.00 3.00 5.888 44 TTTGGAAGTTAGACTTTGACAACA 115 24 0 33.33 58.041 6.00 3.00 5.959 45 GTTTTGGAAGTTAGACTTTGACAACA 117 26 0 34.62 60.009 8.00 5.00 6.009 46 TTTGGAAGTTAGACTTTGACAACAG 115 25 0 36.00 58.960 6.00 5.00 6.040 47 TGGAAGTTAGACTTTGACAACAGAC 113 25 0 40.00 58.927 6.00 5.00 6.073 48 TTTTGGAAGTTAGACTTTGACAACAG 116 26 0 34.62 60.120 6.00 5.00 6.120 49 TTGGAAGTTAGACTTTGACAACAGAC 114 26 0 38.46 60.127 6.00 5.00 6.127 50 GCTTTGCGTTTTGGAAGTTAGACT 124 24 0 41.67 62.180 4.00 4.00 6.180 51 TGGAAGTTAGACTTTGACAACAGACT 113 26 0 38.46 59.802 7.00 7.00 6.198 52 TGCGTTTTGGAAGTTAGACTTTGA 120 24 0 37.50 62.242 6.00 4.00 6.242 53 CTTTGCGTTTTGGAAGTTAGACTTT 123 25 0 36.00 61.265 6.00 4.00 6.265 54 TTGGAAGTTAGACTTTGACAACAG 114 24 0 37.50 57.684 6.00 5.00 6.316 55 CTTTGACAACAGACTCATACAGTGA 102 25 0 40.00 58.507 5.00 2.00 6.493 56 TTTGACAACAGACTCATACAGTGAG 101 25 0 40.00 58.507 5.00 4.00 6.493 57 TTTGACAACAGACTCATACAGTGA 101 24 0 37.50 57.479 5.00 2.00 6.521 58 CTTTGACAACAGACTCATACAGTGAG 102 26 0 42.31 59.446 5.00 4.00 6.554 59 TTTGGAAGTTAGACTTTGACAACAGA 115 26 0 34.62 60.557 6.00 3.00 6.557 60 GACTTTGACAACAGACTCATACAGTG 104 26 0 42.31 59.320 5.00 3.00 6.680 61 ACTTTGACAACAGACTCATACAGTGA 103 26 0 38.46 59.320 5.00 2.00 6.680 62 TTTGACAACAGACTCATACAGTGAGT 101 26 0 38.46 59.320 6.00 6.00 6.680 63 TTGACAACAGACTCATACAGTGAGTT 100 26 0 38.46 59.320 6.00 4.00 6.680 64 TTGACAACAGACTCATACAGTGAG 100 24 0 41.67 57.083 5.00 4.00 6.917 65 TTGACAACAGACTCATACAGTGAGT 100 25 0 40.00 57.993 6.00 6.00 7.007 66 GGAAGTTAGACTTTGACAACAGACTC 112 26 0 42.31 58.988 7.00 5.00 7.012 67 AGACTTTGACAACAGACTCATACAGTG 105 27 0 40.74 60.192 7.00 3.00 7.192 68 ACTTTGACAACAGACTCATACAGTGAG 103 27 0 40.74 60.192 5.00 4.00 7.192 69 CTTTGACAACAGACTCATACAGTGAGT 102 27 0 40.74 60.192 6.00 6.00 7.192 70 GTTTTGGAAGTTAGACTTTGACAAC 117 25 0 36.00 57.612 8.00 8.00 7.388 71 ACTTTGACAACAGACTCATACAGTG 103 25 0 40.00 57.555 5.00 3.00 7.445 72 TTTGACAACAGACTCATACAGTGAGTT 101 27 0 37.04 60.519 6.00 4.00 7.519 73 CGTTTTGGAAGTTAGACTTTGACAAC 118 26 0 38.46 61.521 8.00 8.00 7.521 74 GGAAGTTAGACTTTGACAACAGACT 112 25 0 40.00 57.318 7.00 7.00 7.682 75 GAAGTTAGACTTTGACAACAGACTCA 111 26 0 38.46 58.271 7.00 7.00 7.729 76 TTTGCGTTTTGGAAGTTAGACTTTG 122 25 0 36.00 62.766 6.00 2.00 7.766 77 GTTTTGGAAGTTAGACTTTGACAACAG 117 27 0 37.04 60.782 8.00 5.00 7.782 78 TGCGTTTTGGAAGTTAGACTTTGAC 120 25 0 40.00 62.860 6.00 3.00 7.860 79 GCGTTTTGGAAGTTAGACTTTGACA 119 25 0 40.00 62.860 6.00 4.00 7.860 80 TTGGAAGTTAGACTTTGACAACAGACT 114 27 0 37.04 60.916 7.00 7.00 7.916 81 GACTTTGACAACAGACTCATACAGTGA 104 27 0 40.74 60.988 5.00 2.00 7.988 82 TTTGGAAGTTAGACTTTGACAACAGAC 115 27 0 37.04 61.219 6.00 5.00 8.219 83 AGACTTTGACAACAGACTCATACAGT 105 26 0 38.46 57.680 7.00 3.00 8.320 84 TGGAAGTTAGACTTTGACAACAGACTC 113 27 0 40.74 61.367 7.00 5.00 8.367 85 GGAAGTTAGACTTTGACAACAGACTCA 112 27 0 40.74 61.367 7.00 7.00 8.367 86 GAAGTTAGACTTTGACAACAGACTCAT 111 27 0 37.04 58.586 7.00 5.00 8.414 87 TTTTGGAAGTTAGACTTTGACAACAGA 116 27 0 33.33 61.617 6.00 3.00 8.617 88 TTAGACTTTGACAACAGACTCATACA 107 26 0 34.62 57.298 7.00 1.00 8.702 89 TTAGACTTTGACAACAGACTCATACAG 107 27 0 37.04 58.234 7.00 1.00 8.766 90 TTGACAACAGACTCATACAGTGAGTTG 100 27 0 40.74 61.830 6.00 6.00 8.830 91 GTTAGACTTTGACAACAGACTCATACA 108 27 0 37.04 58.107 7.00 1.00 8.893 92 TAGACTTTGACAACAGACTCATACAGT 106 27 0 37.04 57.767 7.00 3.00 9.233 93 AAGTTAGACTTTGACAACAGACTCATA 110 27 0 33.33 57.047 7.00 3.00 9.953 primer3-2.3.6/test/primer1_list_last/SP01-06.txt.for0000664000077300007730000000743012231156243020730 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGAATCGCAATCAAATTTTTTC 33 22 0 27.27 59.091 6.00 2.00 2.909 1 TGAATCGCAATCAAATTTTTTCT 33 23 0 26.09 59.967 6.00 2.00 3.033 2 ATGAATCGCAATCAAATTTTTTC 32 23 0 26.09 59.374 6.00 2.00 3.626 3 TGAATCGCAATCAAATTTTTT 33 21 0 23.81 57.334 6.00 4.00 3.666 4 TGAATCGCAATCAAATTTTTTCTA 33 24 0 25.00 59.977 6.00 2.00 4.023 5 ATGAATCGCAATCAAATTTTTTCT 32 24 0 25.00 60.199 6.00 2.00 4.199 6 TCGCAATCAAATTTTTTCTATGA 37 23 0 26.09 58.793 6.00 2.00 4.207 7 ATGAATCGCAATCAAATTTTTT 32 22 0 22.73 57.710 6.00 4.00 4.290 8 TCGCAATCAAATTTTTTCTATGAC 37 24 0 29.17 59.542 6.00 1.00 4.458 9 GAATCGCAATCAAATTTTTTCT 34 22 0 27.27 57.467 6.00 2.00 4.533 10 TATGAATCGCAATCAAATTTTTTC 31 24 0 25.00 59.404 6.00 2.00 4.596 11 ATCGCAATCAAATTTTTTCTATGA 36 24 0 25.00 59.083 6.00 3.00 4.917 12 TCGCAATCAAATTTTTTCTATG 37 22 0 27.27 57.077 6.00 2.00 4.923 13 TTATGAATCGCAATCAAATTTTTT 30 24 0 20.83 58.994 6.00 4.00 5.006 14 AATCGCAATCAAATTTTTTCTATGA 35 25 0 24.00 60.202 6.00 2.00 5.202 15 GAATCGCAATCAAATTTTTTCTATG 34 25 0 28.00 60.202 6.00 2.00 5.202 16 TGAATCGCAATCAAATTTTTTCTAT 33 25 0 24.00 60.202 6.00 4.00 5.202 17 ATGAATCGCAATCAAATTTTTTCTA 32 25 0 24.00 60.202 6.00 2.00 5.202 18 TATGAATCGCAATCAAATTTTTTCT 31 25 0 24.00 60.202 6.00 2.00 5.202 19 TATGAATCGCAATCAAATTTTTT 31 23 0 21.74 57.795 6.00 4.00 5.205 20 TTATGAATCGCAATCAAATTTTT 30 23 0 21.74 57.795 6.00 4.00 5.205 21 ATCGCAATCAAATTTTTTCTATGAC 36 25 0 28.00 59.789 6.00 2.00 5.211 22 AATCGCAATCAAATTTTTTCTATG 35 24 0 25.00 58.677 6.00 2.00 5.323 23 GAATCGCAATCAAATTTTTTCTA 34 23 0 26.09 57.561 6.00 2.00 5.439 24 TTATGAATCGCAATCAAATTTTTTC 30 25 0 24.00 60.502 6.00 2.00 5.502 25 ATCGCAATCAAATTTTTTCTATG 36 23 0 26.09 57.456 6.00 2.00 5.544 26 ATTATGAATCGCAATCAAATTTTTT 29 25 0 20.00 59.255 6.00 4.00 5.745 27 AATTATGAATCGCAATCAAATTTTT 28 25 0 20.00 59.255 8.00 4.00 5.745 28 ATTATGAATCGCAATCAAATTTTT 29 24 0 20.83 58.122 6.00 4.00 5.878 29 AATTATGAATCGCAATCAAATTTT 28 24 0 20.83 58.122 8.00 5.00 5.878 30 GAATCGCAATCAAATTTTTTCTAT 34 24 0 25.00 57.900 6.00 4.00 6.100 31 TATGAATCGCAATCAAATTTTTTCTA 31 26 0 23.08 60.204 6.00 2.00 6.204 32 ATGAATCGCAATCAAATTTTTTCTAT 32 26 0 23.08 60.408 6.00 4.00 6.408 33 ATTATGAATCGCAATCAAATTTTTTC 29 26 0 23.08 60.694 6.00 2.00 6.694 34 AATCGCAATCAAATTTTTTCTATGAC 35 26 0 26.92 60.843 6.00 1.00 6.843 35 TTATGAATCGCAATCAAATTTTTTCT 30 26 0 23.08 61.226 6.00 2.00 7.226 36 TATGAATCGCAATCAAATTTTTTCTAT 31 27 0 22.22 60.404 6.00 4.00 7.404 37 TCGCAATCAAATTTTTTCTATGACC 37 25 0 32.00 62.463 6.00 0.00 7.463 38 GAATCGCAATCAAATTTTTTCTATGA 34 26 0 26.92 61.653 6.00 2.00 7.653 39 TTATGAATCGCAATCAAATTTTTTCTA 30 27 0 22.22 61.199 6.00 2.00 8.199 40 ATTATGAATCGCAATCAAATTTTTTCT 29 27 0 22.22 61.382 6.00 2.00 8.382 41 TGAATCGCAATCAAATTTTTTCTATG 33 26 0 26.92 62.397 6.00 2.00 8.397 42 ATCGCAATCAAATTTTTTCTATGACC 36 26 0 30.77 62.574 6.00 0.00 8.574 43 AATTATGAATCGCAATCAAATTTTTTC 28 27 0 22.22 61.652 8.00 2.00 8.652 44 GAATCGCAATCAAATTTTTTCTATGAC 34 27 0 29.63 62.235 6.00 1.00 9.235 45 ATGAATCGCAATCAAATTTTTTCTATG 32 27 0 25.93 62.507 6.00 2.00 9.507 primer3-2.3.6/test/primer_thal_args_input0000664000077300007730000000364412231156245017530 0ustar ioanaioanaPRIMER_TASK=generic PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=4 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_INTERNAL_DNA_CONC=200 PRIMER_INTERNAL_SALT_MONOVALENT=1 PRIMER_INTERNAL_SALT_DIVALENT=3 PRIMER_INTERNAL_MAX_SIZE=32 PRIMER_INTERNAL_OPT_SIZE=30 PRIMER_INTERNAL_MIN_SIZE=28 SEQUENCE_ID=IO_sec_str_thal_args_non_def_non_primer_args_1 SEQUENCE_TEMPLATE=CACAGTTATGCACAGAGCTGCAAACAACTATACATGATATAATATTAGAATGTGTGTACTGCAAGCAACAGTTACTGCGACGTGAGGTATATGACTTTGCTTTTCGGGATTTATGCATAGTATATAGAGATGGGAATCCATATGCTGTATGTGATAAATGTTTAAAGTTTTATTCTAAAATTAGTGAGTATAGACATTATTGTTATAGTGTGTATGGAACAACATTAGAACAGCAATACAACAAACCGTTGTGTGATTTGTTAATTAGGTGTATTAACTGTCAAAAGCCACTGTGTCCTGAAGAAAAGCAAAGACATCTGGACAAAAAGCAAAGATTCCATAATATAAGGGGTCGGTGGACCGGTCGATGTATGTCTTGTTGCAGATCATCAAGAACACGTAGAGAAACCCAGCTGTAATCATGCATGGAGATACACCTACATTGCATGAATATATGTTAGATTTGCAACCAGAGACAACTGATCTCTACTGTTATGAGCAATTAAATGACAGCTCAGAGGAGGAGGATGAAATAGATGGTCCAGCTGGACAAGCAGAACCGGACAGAGCCCATTACAATATTGTAACCTTTTGTTGCAAGTGTGACTCTACGCTTCGGTTGTGCGTACAAAGCACACACGTAGACATTCGTACTTTGGAAGACCTGTTAATGGGCACACTAGGAATTGTGTGCCCCATCTGTTCTCAGAAACCATAATCTACCATGGCTGATCCTGCAGGTACCAATGGGGAAGAGGGTACGGGATGTAATGGATGGTTTTATGTAGAGGCTGTAGTGGAAAAAAAAACAGGGGATGCTATATCAGATGACGAGAACGAAAATGACAGTGATACAGGTGAAGATTTGGTAGATTTTATAGTAAATGATAATGATTATTTAACACAGGCAGAAACAGAGACAGCACATGCGTTGTTTACTGCACAGGAAGCAAAACAACATAGAGATGCAGTACAGGTTC = PRIMER_SALT_MONOVALENT=0.0001 PRIMER_SALT_DIVALENT=2.5 PRIMER_DNA_CONC=205 PRIMER_DNTP_CONC=1.5 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_MIN_THREE_PRIME_DISTANCE=4 SEQUENCE_ID=sec_str_thal_args_def_vs_user SEQUENCE_TEMPLATE=CGCTGCTGCGCTCCAAGCTTTACCGCTTCGCCTGCTGGCTTCGCTAAATTGCGGATTGGAAAATTTTGAAATAAGAACCCCTATCGGGCTGTTATCTGATTATGGTTATTTTGACATAGTTGTATCATCTTAAAAACAAGCATACAATCTGCAAATCTTAGACAAAGCAAAACCCCCGCCAAACGCCAATCTGCACGGGGGTTTCGAGAT = primer3-2.3.6/test/primer_must_use_th_input0000664000077300007730000001711612231156245020122 0ustar ioanaioanaPRIMER_DNTP_CONC=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 SEQUENCE_ID=anyway_1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 PRIMER_PICK_ANYWAY=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 = SEQUENCE_ID=high_tm PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = SEQUENCE_ID=high_tm_and_self_any PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = SEQUENCE_ID=high_tm_and_self_any_and_target PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=20,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = SEQUENCE_ID=high_tm_and_self_any_and_excl_region PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 SEQUENCE_EXCLUDED_REGION=20,1 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = SEQUENCE_ID=high_tm_and_self_any_and_incl_region PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 SEQUENCE_INCLUDED_REGION=1,160 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = SEQUENCE_ID=left_high_tm_and_self_any_and_right PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 = SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc_and_target PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = SEQUENCE_ID=internal_too_short PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccat PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = SEQUENCE_ID=internal_ok PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = SEQUENCE_ID=internal_too_cold PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = SEQUENCE_ID=internal_too_cold2 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=CCTACAGCTGTGGGAAAATG SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=100 PRIMER_MAX_GC=100 = SEQUENCE_ID=mispriming PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=CCTACAGCTGTGGGAAAATG SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=100 PRIMER_MAX_GC=100 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_MAX_LIBRARY_MISPRIMING=8 = primer3-2.3.6/test/primer_high_gc_load_set.set0000664000077300007730000000033012231156245020366 0ustar ioanaioanaPrimer3 File - http://primer3.sourceforge.net P3_FILE_TYPE=settings P3_FILE_ID=Test with higher Tm PRIMER_MIN_GC=62 PRIMER_OPT_GC_PERCENT=75 PRIMER_MAX_GC=80 PRIMER_WT_GC_PERCENT_GT=1.0 PRIMER_WT_GC_PERCENT_LT=1.0 =primer3-2.3.6/test/dpal_test.pl0000664000077300007730000002571212231156245015360 0ustar ioanaioana # Usage: perl dpal_test.pl [ --valgrind ] # This is the driver for tests of dpal (dpal.{c,h}, using in the wrapper # C program ntdpal (coded in ntdpal_main.c) # ====================================================================== # (c) Copyright 1996,1997,1998,1999,2000,2001,2004,2006,2007, 2008 # Whitehead Institute for Biomedical Research, Steve Rozen, # Andreas Untergasser and Helen Skaletsky # All rights reserved. # # This file is part of the primer3 suite and the dpal library. # # The primer3 suite is free software; you can # redistribute it and/or modify it under the terms of the GNU # General Public License as published by the Free Software Foundation; # either version 2 of the License, or (at your option) any later # version. # # This software is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # # You should have received a copy of the GNU General Public License # along with this file (file gpl-2.0.txt in the source distribution); if # not, write to the Free Software Foundation, Inc., 51 Franklin St, # Fifth Floor, Boston, MA 02110-1301 USA # # THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS # "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT # LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR # A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT # OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, # SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT # LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, # DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY # THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT # (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE # OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. # ====================================================================== use strict; use warnings 'all'; use Getopt::Long; use Carp; use POSIX; # For testing wait / system return value, e.g. WIFSIGNALED, WTERMSIG..... use Config; # To get the number for SIGINT our $test_count = 0; our $exit_status = 0; our $do_valgrind; our %signo; our $valgrind_exe = "/usr/local/bin/valgrind"; our $valgrind_format; our $winFlag; our $exe = '../src/ntdpal'; sub perldiff($$); sub main(); # Call system() with warnings turned off; needed for ActiveState / MS Windows. sub _nowarn_system($); main(); sub runtest($$$$$) { my ($desc, $ntdpal_args, $infile, $benchfile, $outfile) = @_; print $desc, '...'; open Q, $infile or confess "open $infile: $!"; # Reopen STDOUT to get all ntdpal output in one file open OLDOUT, ">&STDOUT" or confess "Cannot dup STDOUT: $!"; open STDOUT, '>', $outfile or confess "open STDOUT '>' $outfile: $!"; while (my $in = ) { $test_count++; my $valgrind_prefix = $do_valgrind ? sprintf($valgrind_format, $test_count) : ''; my $cmd = "$valgrind_prefix $exe $ntdpal_args $in"; my $r = _nowarn_system($cmd); } close Q; open STDOUT, ">&OLDOUT" or confess "Cannot dup OLDOUT: $!"; close OLDOUT; my $r = perldiff $benchfile, $outfile; if ($r == 0) { print "[OK]\n"; } else { print "[FAILED]\n"; $exit_status = -1; } } sub main() { my %args; # select STDERR; $| = 1; if (!GetOptions(\%args, 'valgrind', 'windows', )) { print "Usage: $0 [ --valgrind ] [ --windows ]\n"; exit -1; } $winFlag = defined $args{'windows'}; $do_valgrind = $args{'valgrind'}; if ($winFlag && $do_valgrind) { print "$0: Cannot specify both --valgrind and --windows\n"; exit -1; } if ((!-x $valgrind_exe) && ($do_valgrind)) { warn "Cannot find $valgrind_exe; will try `which valgrind`\n"; $valgrind_exe= `which valgrind`; chomp($valgrind_exe); if (!$valgrind_exe || ! -x $valgrind_exe) { die "Cannot execute $valgrind_exe"; } } $valgrind_format = "$valgrind_exe --leak-check=yes " . " --show-reachable=yes --log-file=ntdpal.%0.4d.valg "; if ($winFlag) { $exe = '..\\src\\ntdpal.exe'; } # Look for the ntdpal executable die "Cannot execute $exe" unless -x $exe; my $valgrind_prefix = $do_valgrind ? sprintf($valgrind_format, $test_count) : ''; # ================================================== # First test # Test error handling on over-long input sequence: print 'Error handling of too-long sequence...'; my $cmd = "$valgrind_prefix $exe ACGTGTTCGTCGTAAATAACATGCTATATT GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGGCAAACTGCCGGGTCACCAAGAGAGGCTGATACCGCGGTTCACCC l > dpal.tmp 2>&1"; my $r = _nowarn_system($cmd); open X, 'dpal.tmp'; # Get the test output my @foo = ; # Snarf it close X; # Check the output..... if ($foo[0] eq "Error: Sequence 2 longer than DPAL_MAX_ALIGN and alignment is requested\n") { print "[OK]\n" } else { print "[FAILED]\n"; $exit_status = -1; } # ================================================== # Additional tests using runtest() runtest('Default implementations + alignment', "", "dpal_input", 'dpal_output', 'dpal_output1.tmp'); runtest('Default implementations + NO alignment 1', "-s", 'dpal_input', 'dpal_score_output', 'dpal_score_output1.tmp'); runtest('Default implementations + NO alignment 2', "-s", "dpal_long_input", 'dpal_long_score_output', "dpal_long_score_output1.tmp"); runtest('Force _dpal_generic', '-s -f1', "dpal_input", 'dpal_score_output', "dpal_score_output4.tmp"); runtest('Force _dpal_long_nopath_generic 1', '-s -f2', "dpal_input", 'dpal_score_output', "dpal_score_output2.tmp"); runtest('Force _dpal_long_nopath_generic 2', '-s -f2', "dpal_long_input", 'dpal_long_score_output', "dpal_long_score_output2.tmp"); runtest('Force long maxgap1 functions 1', '-s -f3', "dpal_input", 'dpal_score_output', "dpal_score_output3.tmp"); runtest('Force long maxgap1 functions 2', '-s -f3', "dpal_long_input", 'dpal_long_score_output', "dpal_long_score_output3.tmp"); # ================================================== # If we were running under valgrind to look for memory-related # errors (reading uninitialized memory, writing off the end of # an array, etc) or leaks, then we look through the valgrind # logs to summarize errors and leaks. if ($do_valgrind) { # Assume this is Unix/Linux envrionment, so # we have grep. my $r = system "grep ERROR ntdpal.*.*valg* | grep -v 'ERROR SUMMARY: 0 errors'"; if (!$r) { # !$r because grep returns 0 if something is found, # and if something is found, we have a problem. $exit_status = -1; } $r = system "grep 'definitely lost' ntdpal.*.*valg* | grep -v '0 bytes'"; if (!$r) { $exit_status = -1; } $r = system "grep 'possibly lost' ntdpal.*.*valg* | grep -v '0 bytes'"; if (!$r) { $exit_status = -1; } } exit $exit_status; } # Usage: perldiff("filename1", "filename2") # Return 0 if no differences are found, # othewise return 1; sub perldiff($$) { my ($f1, $f2) = @_; open F1, $f1 or die "open $f1: $!"; open F2, $f2 or die "open $f2: $!"; my @f1 = ; my @f2 = ; # If different number of lines, return FAIL. if (@f1 != @f2) { print "Different number of lines\n"; return 1; } # check for differences on the lines, themselves my $linenumber = 0; my $line_end_diff = 0; # iterate using lines in file1 while (@f1) { $linenumber++; # get the lines from each respective file my $l1 = shift @f1; my $l2 = shift @f2; my $l1_orig = $l1; my $l2_orig = $l2; $linenumber++; # Check for difference between two edited lines (line by line) if ($l1 ne $l2) { print "Difference found at line $linenumber:\n< $l1_orig\n> $l2_orig\n"; return 1; } } return 0; } sub _nowarn_system($) { my $cmd = shift; no warnings 'all'; my $r = system $cmd; my $r2 = $?; if (!$winFlag && WIFEXITED($r2)) { $r = WEXITSTATUS($r2); if (defined $signo{'INT'}) { if ($r == $signo{'INT'}) { print "\nCommand: $cmd\n"; print "generated exit value $r\n"; print "Presumably caused by catching SIGINT\n"; print "Tests halted\n\n"; print "\nWARNING: not all tests executed ... [FAILED]\n"; exit; } } } if (!$winFlag && WIFSIGNALED($r2)) { my $r2 = WTERMSIG($r2); print "Exited with signal $r2\n"; } $r; } primer3-2.3.6/test/primer_list_last/0000775000077300007730000000000012231156372016412 5ustar ioanaioanaprimer3-2.3.6/test/primer_list_last/SP01-06-with-repeat2.for0000664000077300007730000001074612231156244022347 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self lib qual- # sequence start ln N GC% Tm any end sim lity 0 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 10.00 2.909 1 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 10.00 3.033 2 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 10.00 3.626 3 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 10.00 4.023 4 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 10.00 4.199 5 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 10.00 4.207 6 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 10.00 4.458 7 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 10.00 4.533 8 GAATCGCAATCAAATTTTTTC 33 21 0 28.57 56.457 6.00 2.00 9.00 4.543 9 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 10.00 4.596 10 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 10.00 4.923 11 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 10.00 5.202 12 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 10.00 5.202 13 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 10.00 5.202 14 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 10.00 5.202 15 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 10.00 5.211 16 AATCGCAATCAAATTTTTTCT 34 21 0 23.81 55.693 6.00 2.00 10.00 5.307 17 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 10.00 5.323 18 AATCGCAATCAAATTTTTTC 34 20 0 25.00 54.571 6.00 2.00 9.00 5.429 19 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 9.00 5.439 20 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 10.00 5.502 21 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 10.00 5.544 22 ATCGCAATCAAATTTTTTCT 35 20 0 25.00 54.168 6.00 2.00 10.00 5.832 23 AATCGCAATCAAATTTTTTCTA 34 22 0 22.73 55.850 6.00 2.00 9.00 6.150 24 TCGCAATCAAATTTTTTCTA 36 20 0 25.00 53.814 6.00 2.00 9.00 6.186 25 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 10.00 6.204 26 TCGCAATCAAATTTTTTCTAT 36 21 0 23.81 54.389 6.00 2.00 9.00 6.611 27 ATCGCAATCAAATTTTTTCTA 35 21 0 23.81 54.389 6.00 2.00 9.00 6.611 28 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 10.00 6.694 29 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 10.00 6.843 30 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 10.00 7.226 31 TCGCAATCAAATTTTTTCT 36 19 0 26.32 53.559 6.00 2.00 10.00 7.441 32 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 11.00 7.463 33 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 10.00 7.653 34 ATCGCAATCAAATTTTTTC 35 19 0 26.32 52.911 6.00 2.00 9.00 8.089 35 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 10.00 8.199 36 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 10.00 8.382 37 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 10.00 8.397 38 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 11.00 8.574 39 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 10.00 8.652 40 TCGCAATCAAATTTTTTCTATGACCT 36 26 0 30.77 63.123 6.00 1.00 10.00 9.123 41 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 10.00 9.235 42 AATTATGAATCGCAATCAA 27 19 0 26.32 51.609 5.00 2.00 10.00 9.391 43 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 10.00 9.507 44 TCGCAATCAAATTTTTTC 36 18 0 27.78 52.197 6.00 2.00 9.00 9.803 45 ATCGCAATCAAATTTTTTCTATGACCT 35 27 0 29.63 63.204 6.00 1.00 10.00 10.204 46 AATCGCAATCAAATTTTTTCTATGACC 34 27 0 29.63 63.467 6.00 0.00 11.00 10.467 47 TGAATCGCAATCAAATTTTTTCTATGA 32 27 0 25.93 63.763 6.00 2.00 11.00 10.763 48 TCGCAATCAAATTTTTTCTATGACCTG 36 27 0 33.33 65.188 6.00 1.00 9.00 12.188 49 TATGAATCGCAATCAAAT 30 18 0 27.78 49.586 4.00 2.00 10.00 12.414 50 ATTATGAATCGCAATCAA 28 18 0 27.78 49.586 5.00 2.00 10.00 12.414 primer3-2.3.6/test/primer_list_last/SP01-06-with-repeat1.rev0000664000077300007730000003634712231156244022361 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self lib qual- # sequence start ln N GC% Tm any end sim lity 0 GTGCTTTGCGTTTTGGAAGT 125 20 0 45.00 60.292 3.00 1.00 9.00 0.292 1 GTGCTTTGCGTTTTGGAAG 125 19 0 47.37 59.454 3.00 0.00 8.00 1.546 2 TGCTTTGCGTTTTGGAAGT 124 19 0 42.11 59.454 3.00 1.00 9.00 1.546 3 TGCTTTGCGTTTTGGAAGTTA 124 21 0 38.10 60.764 3.00 2.00 9.00 1.764 4 GCTTTGCGTTTTGGAAGTTA 123 20 0 40.00 58.097 3.00 2.00 9.00 1.903 5 GCTTTGCGTTTTGGAAGTTAG 123 21 0 42.86 59.065 3.00 0.00 9.00 1.935 6 GAGCGTGCTTTGCGTTTT 129 18 0 50.00 60.142 4.00 0.00 9.00 2.142 7 GCTTTGCGTTTTGGAAGTTAGA 123 22 0 40.91 60.748 3.00 1.00 8.00 2.748 8 AGCGTGCTTTGCGTTTTG 128 18 0 50.00 61.131 4.00 0.00 8.00 3.131 9 TGCTTTGCGTTTTGGAAG 124 18 0 44.44 58.546 3.00 0.00 8.00 3.454 10 GTGCTTTGCGTTTTGGAAGTTA 125 22 0 40.91 61.484 3.00 2.00 9.00 3.484 11 TGCTTTGCGTTTTGGAAGTTAG 124 22 0 40.91 61.593 3.00 0.00 10.00 3.593 12 GTGCTTTGCGTTTTGGAA 125 18 0 44.44 58.389 2.00 2.00 9.00 3.611 13 TGCGTTTTGGAAGTTAGACTTTG 119 23 0 39.13 60.658 6.00 2.00 9.00 3.658 14 CTTTGCGTTTTGGAAGTTAGA 122 21 0 38.10 57.272 3.00 1.00 8.00 3.728 15 TTTGCGTTTTGGAAGTTAGA 121 20 0 35.00 56.191 3.00 1.00 8.00 3.809 16 CTTTGCGTTTTGGAAGTTAGAC 122 22 0 40.91 58.127 3.00 2.00 9.00 3.873 17 GCGTTTTGGAAGTTAGACTTTG 118 22 0 40.91 58.127 6.00 2.00 9.00 3.873 18 TTTGCGTTTTGGAAGTTAGAC 121 21 0 38.10 57.124 3.00 2.00 9.00 3.876 19 CGTGCTTTGCGTTTTGGA 126 18 0 50.00 61.944 2.00 0.00 8.00 3.944 20 CGTGCTTTGCGTTTTGGAAG 126 20 0 50.00 63.967 3.00 0.00 8.00 3.967 21 GAGCGTGCTTTGCGTTTTG 129 19 0 52.63 62.977 4.00 0.00 8.00 3.977 22 CGTGCTTTGCGTTTTGGAA 126 19 0 47.37 63.187 2.00 2.00 9.00 4.187 23 TTGCGTTTTGGAAGTTAGAC 120 20 0 40.00 55.619 3.00 2.00 9.00 4.381 24 GCTTTGCGTTTTGGAAGTTAGAC 123 23 0 43.48 61.438 3.00 2.00 9.00 4.438 25 GGAGCGTGCTTTGCGTTT 130 18 0 55.56 62.465 4.00 0.00 8.00 4.465 26 CTTTGCGTTTTGGAAGTTAG 122 20 0 40.00 55.385 3.00 0.00 8.00 4.615 27 GGAGCGTGCTTTGCGTTTT 130 19 0 52.63 63.650 4.00 0.00 9.00 4.650 28 GATCGGGAGCGTGCTTTG 135 18 0 61.11 62.907 4.00 0.00 8.00 4.907 29 GTGCTTTGCGTTTTGGAAGTTAG 125 23 0 43.48 62.256 3.00 0.00 10.00 5.256 30 GCTTTGCGTTTTGGAAGT 123 18 0 44.44 56.483 3.00 1.00 9.00 5.517 31 AGCGTGCTTTGCGTTTTGG 128 19 0 52.63 64.584 4.00 0.00 9.00 5.584 32 CGTGCTTTGCGTTTTGGAAGT 126 21 0 47.62 64.590 3.00 1.00 9.00 5.590 33 TTGCGTTTTGGAAGTTAGACTTTG 120 24 0 37.50 61.761 6.00 2.00 9.00 5.761 34 GCGTGCTTTGCGTTTTGG 127 18 0 55.56 63.814 2.00 0.00 9.00 5.814 35 TGCTTTGCGTTTTGGAAGTTAGA 124 23 0 39.13 63.165 3.00 1.00 9.00 6.165 36 GGAGCGTGCTTTGCGTTTTG 130 20 0 55.00 66.221 4.00 0.00 8.00 6.221 37 GAGCGTGCTTTGCGTTTTGG 129 20 0 55.00 66.221 4.00 0.00 9.00 6.221 38 AGCGTGCTTTGCGTTTTGGA 128 20 0 50.00 66.221 4.00 0.00 8.00 6.221 39 TTGCGTTTTGGAAGTTAGA 120 19 0 36.84 54.570 3.00 1.00 8.00 6.430 40 CTTTGACAACAGACTCATACAGTGA 101 25 0 40.00 58.507 5.00 2.00 10.00 6.493 41 TTTGACAACAGACTCATACAGTGA 100 24 0 37.50 57.479 5.00 2.00 9.00 6.521 42 GCGTGCTTTGCGTTTTGGAA 127 20 0 50.00 66.535 2.00 2.00 9.00 6.535 43 GCGTGCTTTGCGTTTTGGA 127 19 0 52.63 65.553 2.00 0.00 8.00 6.553 44 CGTTTTGGAAGTTAGACTTTG 117 21 0 38.10 54.425 6.00 2.00 9.00 6.575 45 ACTTTGACAACAGACTCATACAGTGA 102 26 0 38.46 59.320 5.00 2.00 10.00 6.680 46 GGGAGCGTGCTTTGCGTT 131 18 0 61.11 64.759 4.00 0.00 8.00 6.759 47 GGGAGCGTGCTTTGCGTTTT 131 20 0 55.00 66.761 4.00 0.00 9.00 6.761 48 ATCGGGAGCGTGCTTTGC 134 18 0 61.11 64.764 4.00 2.00 7.00 6.764 49 GGGAGCGTGCTTTGCGTTT 131 19 0 57.89 65.814 4.00 0.00 8.00 6.814 50 CTTTGCGTTTTGGAAGTTA 122 19 0 36.84 54.178 3.00 2.00 9.00 6.822 51 TTTGCGTTTTGGAAGTTAG 121 19 0 36.84 54.178 3.00 0.00 8.00 6.822 52 TGCGTTTTGGAAGTTAGAC 119 19 0 42.11 53.939 3.00 2.00 9.00 7.061 53 TTGACAACAGACTCATACAGTGA 99 23 0 39.13 55.925 5.00 2.00 9.00 7.075 54 GATCGGGAGCGTGCTTTGC 135 19 0 63.16 66.488 4.00 2.00 8.00 7.488 55 GTGCTTTGCGTTTTGGAAGTTAGA 125 24 0 41.67 63.758 3.00 1.00 9.00 7.758 56 TGCTTTGCGTTTTGGAAGTTAGAC 124 24 0 41.67 63.758 3.00 2.00 10.00 7.758 57 TTTGCGTTTTGGAAGTTAGACTTTG 121 25 0 36.00 62.766 6.00 2.00 9.00 7.766 58 GACTTTGACAACAGACTCATACAGTGA 103 27 0 40.74 60.988 5.00 2.00 10.00 7.988 59 AGCGTGCTTTGCGTTTTGGAA 128 21 0 47.62 67.128 4.00 2.00 9.00 8.128 60 GCGTGCTTTGCGTTTTGGAAG 127 21 0 52.38 67.128 3.00 0.00 8.00 8.128 61 AGACTTTGACAACAGACTCATACAG 104 25 0 40.00 56.820 7.00 1.00 9.00 8.180 62 AGACTTTGACAACAGACTCATACA 104 24 0 37.50 55.732 7.00 0.00 10.00 8.268 63 GACTTTGACAACAGACTCATACAG 103 24 0 41.67 55.732 5.00 1.00 9.00 8.268 64 CGTGCTTTGCGTTTTGGAAGTTA 126 23 0 43.48 65.376 3.00 2.00 9.00 8.376 65 GACTTTGACAACAGACTCATACA 103 23 0 39.13 54.538 5.00 0.00 10.00 8.462 66 TTAGACTTTGACAACAGACTCATACA 106 26 0 34.62 57.298 7.00 1.00 10.00 8.702 67 GAGCGTGCTTTGCGTTTTGGA 129 21 0 52.38 67.764 4.00 0.00 8.00 8.764 68 TTAGACTTTGACAACAGACTCATACAG 106 27 0 37.04 58.234 7.00 1.00 9.00 8.766 69 GTTAGACTTTGACAACAGACTCATACA 107 27 0 37.04 58.107 7.00 1.00 10.00 8.893 70 TAGACTTTGACAACAGACTCATACAG 105 26 0 38.46 56.940 7.00 1.00 9.00 9.060 71 TAGACTTTGACAACAGACTCATACA 105 25 0 36.00 55.897 7.00 0.00 10.00 9.103 72 TTTGCGTTTTGGAAGTTA 121 18 0 33.33 52.848 3.00 2.00 9.00 9.152 73 TGCGTTTTGGAAGTTAGA 119 18 0 38.89 52.751 3.00 1.00 8.00 9.249 74 ACTTTGACAACAGACTCATACAG 102 23 0 39.13 53.733 5.00 1.00 9.00 9.267 75 GTGCTTTGCGTTTTGGAAGTTAGAC 125 25 0 44.00 64.312 3.00 2.00 10.00 9.312 76 CTTTGACAACAGACTCATACAG 101 22 0 40.91 52.603 5.00 1.00 9.00 9.397 77 CTTTGCGTTTTGGAAGTTAGACTTTG 122 26 0 38.46 63.414 6.00 2.00 9.00 9.414 78 ATCGGGAGCGTGCTTTGCGT 134 20 0 60.00 69.470 4.00 0.00 7.00 9.470 79 ACTTTGACAACAGACTCATACA 102 22 0 36.36 52.399 5.00 0.00 10.00 9.601 80 GCGTGCTTTGCGTTTTGGAAGT 127 22 0 50.00 67.608 3.00 1.00 9.00 9.608 81 AGACTTTGACAACAGACTCA 104 20 0 40.00 50.384 7.00 1.00 9.00 9.616 82 TTGCGTTTTGGAAGTTAG 120 18 0 38.89 52.384 3.00 0.00 8.00 9.616 83 AGCGTGCTTTGCGTTTTGGAAG 128 22 0 50.00 67.671 4.00 0.00 8.00 9.671 84 AGACTTTGACAACAGACTCAT 104 21 0 38.10 51.260 7.00 2.00 9.00 9.740 85 CGGGAGCGTGCTTTGCGTTT 132 20 0 60.00 69.763 4.00 0.00 8.00 9.763 86 CTTTGACAACAGACTCATACA 101 21 0 38.10 51.152 5.00 0.00 10.00 9.848 87 TTTGACAACAGACTCATACAG 100 21 0 38.10 51.152 5.00 1.00 9.00 9.848 88 CGTGCTTTGCGTTTTGGAAGTTAG 126 24 0 45.83 65.955 3.00 0.00 10.00 9.955 89 CGGGAGCGTGCTTTGCGTT 132 19 0 63.16 68.983 4.00 0.00 8.00 9.983 90 ATCGGGAGCGTGCTTTGCG 134 19 0 63.16 69.025 4.00 2.00 8.00 10.025 91 GGGAGCGTGCTTTGCGTTTTG 131 21 0 57.14 69.108 4.00 0.00 8.00 10.108 92 GGAGCGTGCTTTGCGTTTTGG 130 21 0 57.14 69.108 4.00 0.00 9.00 10.108 93 CGGGAGCGTGCTTTGCGT 132 18 0 66.67 68.116 4.00 0.00 7.00 10.116 94 AGACTTTGACAACAGACTCATAC 104 23 0 39.13 52.792 7.00 0.00 9.00 10.208 95 GACTTTGACAACAGACTCAT 103 20 0 40.00 49.674 5.00 2.00 9.00 10.326 96 TTAGACTTTGACAACAGACTCATAC 106 25 0 36.00 54.653 7.00 1.00 9.00 10.347 97 TAGACTTTGACAACAGACTCAT 105 22 0 36.36 51.625 7.00 2.00 9.00 10.375 98 AGACTTTGACAACAGACTCATA 104 22 0 36.36 51.625 7.00 2.00 8.00 10.375 99 TTTGACAACAGACTCATACA 100 20 0 35.00 49.540 5.00 0.00 10.00 10.460 100 TCGGGAGCGTGCTTTGCGTT 133 20 0 60.00 70.467 4.00 0.00 8.00 10.467 101 GATCGGGAGCGTGCTTTGCG 135 20 0 65.00 70.520 4.00 2.00 9.00 10.520 102 GAGCGTGCTTTGCGTTTTGGAA 129 22 0 50.00 68.571 4.00 2.00 9.00 10.571 103 GACTTTGACAACAGACTCATAC 103 22 0 40.91 51.414 5.00 0.00 9.00 10.586 104 TCGGGAGCGTGCTTTGCGT 133 19 0 63.16 69.707 4.00 0.00 7.00 10.707 105 GTTTTGGAAGTTAGACTTTG 116 20 0 35.00 49.271 6.00 2.00 9.00 10.729 106 GACTTTGACAACAGACTCATA 103 21 0 38.10 50.125 5.00 2.00 8.00 10.875 107 TAGACTTTGACAACAGACTCATAC 105 24 0 37.50 53.072 7.00 0.00 9.00 10.928 108 TTGACAACAGACTCATACAG 99 20 0 40.00 49.008 5.00 1.00 9.00 10.992 109 TAGACTTTGACAACAGACTCATA 105 23 0 34.78 51.962 7.00 2.00 8.00 11.038 110 AGCGTGCTTTGCGTTTTGGAAGT 128 23 0 47.83 68.114 4.00 1.00 9.00 11.114 111 ATCGGGAGCGTGCTTTGCGTT 134 21 0 57.14 70.204 4.00 0.00 8.00 11.204 112 TCGGGAGCGTGCTTTGCG 133 18 0 66.67 69.247 4.00 2.00 7.00 11.247 113 GCGTTTTGGAAGTTAGAC 118 18 0 44.44 50.670 3.00 2.00 9.00 11.330 114 CGGGAGCGTGCTTTGCGTTTT 132 21 0 57.14 70.468 4.00 0.00 9.00 11.468 115 GATCGGGAGCGTGCTTTGCGT 135 21 0 61.90 70.899 4.00 0.00 8.00 11.899 116 ACTTTGACAACAGACTCATAC 102 21 0 38.10 49.101 5.00 0.00 9.00 11.899 117 GAGCGTGCTTTGCGTTTTGGAAG 129 23 0 52.17 69.042 4.00 0.00 9.00 12.042 118 GCGTGCTTTGCGTTTTGGAAGTTA 127 24 0 45.83 68.126 3.00 2.00 9.00 12.126 119 TCGGGAGCGTGCTTTGCGTTT 133 21 0 57.14 71.153 4.00 0.00 9.00 12.153 120 CGTGCTTTGCGTTTTGGAAGTTAGA 126 25 0 44.00 67.289 3.00 1.00 9.00 12.289 121 ACTTTGACAACAGACTCATA 102 20 0 35.00 47.663 5.00 2.00 8.00 12.337 122 CTTTGACAACAGACTCATAC 101 20 0 40.00 47.663 5.00 0.00 9.00 12.337 123 GACTTTGACAACAGACTCA 103 19 0 42.11 48.639 5.00 1.00 9.00 12.361 124 GGAGCGTGCTTTGCGTTTTGGA 130 22 0 54.55 70.490 4.00 0.00 9.00 12.490 125 ATCGGGAGCGTGCTTTGCGTTT 134 22 0 54.55 70.870 4.00 0.00 9.00 12.870 126 GCTTTGCGTTTTGGAAGTTAGACTTTG 123 27 0 40.74 66.026 6.00 2.00 9.00 13.026 127 TTTTGGAAGTTAGACTTTG 115 19 0 31.58 47.928 6.00 2.00 9.00 13.072 128 GAGCGTGCTTTGCGTTTTGGAAGT 129 24 0 50.00 69.429 4.00 1.00 9.00 13.429 129 GATCGGGAGCGTGCTTTGCGTT 135 22 0 59.09 71.545 4.00 0.00 9.00 13.545 130 AGCGTGCTTTGCGTTTTGGAAGTTA 128 25 0 44.00 68.576 4.00 2.00 9.00 13.576 131 GCGTGCTTTGCGTTTTGGAAGTTAG 127 25 0 48.00 68.576 3.00 0.00 10.00 13.576 132 GGGAGCGTGCTTTGCGTTTTGG 131 22 0 59.09 71.695 4.00 0.00 9.00 13.695 133 CGTGCTTTGCGTTTTGGAAGTTAGAC 126 26 0 46.15 67.709 3.00 2.00 10.00 13.709 134 TCGGGAGCGTGCTTTGCGTTTT 133 22 0 54.55 71.774 4.00 0.00 9.00 13.774 135 TTGACAACAGACTCATACA 99 19 0 36.84 47.159 5.00 0.00 10.00 13.841 136 ACTTTGACAACAGACTCAT 102 19 0 36.84 47.081 5.00 2.00 9.00 13.919 137 GGAGCGTGCTTTGCGTTTTGGAA 130 23 0 52.17 71.131 4.00 2.00 9.00 14.131 138 ATCGGGAGCGTGCTTTGCGTTTT 134 23 0 52.17 71.476 4.00 0.00 9.00 14.476 139 CGGGAGCGTGCTTTGCGTTTTG 132 22 0 59.09 72.594 4.00 0.00 8.00 14.594 140 CTTTGACAACAGACTCATA 101 19 0 36.84 46.089 5.00 2.00 8.00 14.911 141 AGCGTGCTTTGCGTTTTGGAAGTTAG 128 26 0 46.15 68.993 4.00 0.00 10.00 14.993 142 GATCGGGAGCGTGCTTTGCGTTT 135 23 0 56.52 72.132 4.00 0.00 10.00 15.132 143 TTTGACAACAGACTCATAC 100 19 0 36.84 45.794 5.00 0.00 9.00 15.206 144 GGAGCGTGCTTTGCGTTTTGGAAG 130 24 0 54.17 71.481 4.00 0.00 10.00 15.481 145 GAGCGTGCTTTGCGTTTTGGAAGTTA 129 26 0 46.15 69.786 4.00 2.00 9.00 15.786 146 GCGTGCTTTGCGTTTTGGAAGTTAGA 127 26 0 46.15 69.786 3.00 1.00 9.00 15.786 147 GGGAGCGTGCTTTGCGTTTTGGA 131 23 0 56.52 72.940 4.00 0.00 9.00 15.940 148 ACTTTGACAACAGACTCA 102 18 0 38.89 45.825 5.00 1.00 9.00 16.175 149 TTTGGAAGTTAGACTTTG 114 18 0 33.33 45.649 6.00 2.00 8.00 16.351 150 CTTTGACAACAGACTCAT 101 18 0 38.89 45.402 5.00 2.00 9.00 16.598 151 GATCGGGAGCGTGCTTTGCGTTTT 135 24 0 54.17 72.668 4.00 0.00 9.00 16.668 152 GGAGCGTGCTTTGCGTTTTGGAAGT 130 25 0 52.00 71.774 4.00 1.00 9.00 16.774 153 TCGGGAGCGTGCTTTGCGTTTTG 133 23 0 56.52 73.813 4.00 0.00 8.00 16.813 154 GCGTGCTTTGCGTTTTGGAAGTTAGAC 127 27 0 48.15 70.114 3.00 2.00 10.00 17.114 155 GAGCGTGCTTTGCGTTTTGGAAGTTAG 129 27 0 48.15 70.152 4.00 0.00 10.00 17.152 156 AGCGTGCTTTGCGTTTTGGAAGTTAGA 128 27 0 44.44 70.152 4.00 1.00 9.00 17.152 157 ATCGGGAGCGTGCTTTGCGTTTTG 134 24 0 54.17 73.434 4.00 0.00 8.00 17.434 158 GGGAGCGTGCTTTGCGTTTTGGAA 131 24 0 54.17 73.443 4.00 2.00 9.00 17.443 159 CGGGAGCGTGCTTTGCGTTTTGG 132 23 0 60.87 74.875 4.00 0.00 9.00 17.875 160 TTTGACAACAGACTCATA 100 18 0 33.33 44.031 5.00 2.00 8.00 17.969 161 GGGAGCGTGCTTTGCGTTTTGGAAG 131 25 0 56.00 73.692 4.00 0.00 10.00 18.692 162 TTGACAACAGACTCATAC 99 18 0 38.89 43.056 5.00 0.00 9.00 18.944 163 GGAGCGTGCTTTGCGTTTTGGAAGTTA 130 27 0 48.15 71.956 4.00 2.00 9.00 18.956 164 GATCGGGAGCGTGCTTTGCGTTTTG 135 25 0 56.00 74.552 4.00 0.00 8.00 19.552 165 GGGAGCGTGCTTTGCGTTTTGGAAGT 131 26 0 53.85 73.906 4.00 1.00 9.00 19.906 166 TCGGGAGCGTGCTTTGCGTTTTGG 133 24 0 58.33 75.980 4.00 0.00 9.00 19.980 167 CGGGAGCGTGCTTTGCGTTTTGGA 132 24 0 58.33 75.980 4.00 0.00 9.00 19.980 168 ATCGGGAGCGTGCTTTGCGTTTTGG 134 25 0 56.00 75.528 4.00 0.00 9.00 20.528 169 CGGGAGCGTGCTTTGCGTTTTGGAA 132 25 0 56.00 76.331 4.00 2.00 9.00 21.331 170 TCGGGAGCGTGCTTTGCGTTTTGGA 133 25 0 56.00 77.032 4.00 0.00 9.00 22.032 171 CGGGAGCGTGCTTTGCGTTTTGGAAG 132 26 0 57.69 76.468 4.00 0.00 10.00 22.468 172 GATCGGGAGCGTGCTTTGCGTTTTGG 135 26 0 57.69 76.548 4.00 0.00 9.00 22.548 173 ATCGGGAGCGTGCTTTGCGTTTTGGA 134 26 0 53.85 76.548 4.00 0.00 9.00 22.548 174 TCGGGAGCGTGCTTTGCGTTTTGGAA 133 26 0 53.85 77.333 4.00 2.00 9.00 23.333 175 CGGGAGCGTGCTTTGCGTTTTGGAAGT 132 27 0 55.56 76.595 4.00 1.00 9.00 23.595 176 ATCGGGAGCGTGCTTTGCGTTTTGGAA 134 27 0 51.85 76.856 4.00 2.00 9.00 23.856 177 TCGGGAGCGTGCTTTGCGTTTTGGAAG 133 27 0 55.56 77.432 4.00 0.00 10.00 24.432 178 GATCGGGAGCGTGCTTTGCGTTTTGGA 135 27 0 55.56 77.522 4.00 0.00 9.00 24.522 primer3-2.3.6/test/primer_list_last/SP01-06-with-repeat1.int0000664000077300007730000003103312231156244022342 0ustar ioanaioanaACCEPTABLE INTERNAL OLIGOS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 AAACGCAAAGCACGCTCC 113 18 0 55.56 62.465 4.00 0.00 4.465 1 AAAACGCAAAGCACGCTC 112 18 0 50.00 60.142 4.00 2.00 2.142 2 CAAAACGCAAAGCACGCTC 111 19 0 52.63 62.977 4.00 2.00 3.977 3 TCCAAAACGCAAAGCACG 109 18 0 50.00 61.944 2.00 2.00 3.944 4 TTCCAAAACGCAAAGCAC 108 18 0 44.44 58.389 2.00 0.00 3.611 5 CTTCCAAAACGCAAAGCAC 107 19 0 47.37 59.454 3.00 0.00 1.546 6 ACTTCCAAAACGCAAAGCAC 106 20 0 45.00 60.292 3.00 0.00 0.292 7 AACTTCCAAAACGCAAAGCAC 105 21 0 42.86 61.528 3.00 0.00 2.528 8 TAACTTCCAAAACGCAAAGCAC 104 22 0 40.91 61.484 3.00 0.00 3.484 9 CTAACTTCCAAAACGCAAAGCAC 103 23 0 43.48 62.256 3.00 0.00 5.256 10 CTTCCAAAACGCAAAGCA 107 18 0 44.44 58.546 3.00 0.00 3.454 11 ACTTCCAAAACGCAAAGCA 106 19 0 42.11 59.454 3.00 0.00 1.546 12 AACTTCCAAAACGCAAAGCA 105 20 0 40.00 60.782 3.00 0.00 0.782 13 TAACTTCCAAAACGCAAAGCA 104 21 0 38.10 60.764 3.00 0.00 1.764 14 CTAACTTCCAAAACGCAAAGCA 103 22 0 40.91 61.593 3.00 0.00 3.593 15 AACTTCCAAAACGCAAAGC 105 19 0 42.11 58.013 3.00 2.00 2.987 16 TAACTTCCAAAACGCAAAGC 104 20 0 40.00 58.097 3.00 2.00 1.903 17 CTAACTTCCAAAACGCAAAGC 103 21 0 42.86 59.065 3.00 2.00 1.935 18 TCTAACTTCCAAAACGCAAAGC 102 22 0 40.91 60.748 3.00 2.00 2.748 19 GTCTAACTTCCAAAACGCAAAGC 101 23 0 43.48 61.438 3.00 2.00 4.438 20 AGTCTAACTTCCAAAACGCAAAGC 100 24 0 41.67 62.180 4.00 2.00 6.180 21 TCTAACTTCCAAAACGCAAAG 102 21 0 38.10 57.272 3.00 0.00 3.728 22 GTCTAACTTCCAAAACGCAAAG 101 22 0 40.91 58.127 3.00 0.00 3.873 23 AGTCTAACTTCCAAAACGCAAAG 100 23 0 39.13 59.045 4.00 0.00 3.955 24 AAGTCTAACTTCCAAAACGCAAAG 99 24 0 37.50 60.206 6.00 0.00 4.206 25 AAAGTCTAACTTCCAAAACGCAAAG 98 25 0 36.00 61.265 6.00 0.00 6.265 26 GTCTAACTTCCAAAACGCAAA 101 21 0 38.10 57.124 3.00 0.00 3.876 27 AGTCTAACTTCCAAAACGCAAA 100 22 0 36.36 58.127 4.00 0.00 3.873 28 AAGTCTAACTTCCAAAACGCAAA 99 23 0 34.78 59.378 6.00 0.00 3.622 29 AAAGTCTAACTTCCAAAACGCAAA 98 24 0 33.33 60.517 6.00 0.00 4.517 30 CAAAGTCTAACTTCCAAAACGCAAA 97 25 0 36.00 62.766 6.00 0.00 7.766 31 AAGTCTAACTTCCAAAACGCAA 99 22 0 36.36 58.127 6.00 0.00 3.873 32 AAAGTCTAACTTCCAAAACGCAA 98 23 0 34.78 59.378 6.00 0.00 3.622 33 CAAAGTCTAACTTCCAAAACGCAA 97 24 0 37.50 61.761 6.00 0.00 5.761 34 AAAGTCTAACTTCCAAAACGCA 98 22 0 36.36 58.127 6.00 0.00 3.873 35 CAAAGTCTAACTTCCAAAACGCA 97 23 0 39.13 60.658 6.00 0.00 3.658 36 TCAAAGTCTAACTTCCAAAACGCA 96 24 0 37.50 62.242 6.00 0.00 6.242 37 GTCAAAGTCTAACTTCCAAAACGCA 95 25 0 40.00 62.860 6.00 0.00 7.860 38 CAAAGTCTAACTTCCAAAACGC 97 22 0 40.91 58.127 6.00 2.00 3.873 39 TCAAAGTCTAACTTCCAAAACGC 96 23 0 39.13 59.821 6.00 2.00 3.179 40 GTCAAAGTCTAACTTCCAAAACGC 95 24 0 41.67 60.537 6.00 2.00 4.537 41 TGTCAAAGTCTAACTTCCAAAACGC 94 25 0 40.00 62.860 6.00 2.00 7.860 42 GTCAAAGTCTAACTTCCAAAACG 95 23 0 39.13 57.204 6.00 2.00 5.796 43 TGTCAAAGTCTAACTTCCAAAACG 94 24 0 37.50 59.738 6.00 2.00 4.262 44 TTGTCAAAGTCTAACTTCCAAAACG 93 25 0 36.00 60.875 6.00 2.00 5.875 45 CTGTTGTCAAAGTCTAACTTCCAA 90 24 0 37.50 57.684 6.00 2.00 6.316 46 TCTGTTGTCAAAGTCTAACTTCCAA 89 25 0 36.00 59.394 6.00 2.00 5.606 47 GTCTGTTGTCAAAGTCTAACTTCCAA 88 26 0 38.46 60.127 6.00 2.00 6.127 48 AGTCTGTTGTCAAAGTCTAACTTCCAA 87 27 0 37.04 60.916 7.00 2.00 7.916 49 AGTCTGTTGTCAAAGTCTAACTTCC 87 25 0 40.00 57.318 7.00 2.00 7.682 50 GAGTCTGTTGTCAAAGTCTAACTTCC 86 26 0 42.31 58.988 7.00 2.00 7.012 51 TGAGTCTGTTGTCAAAGTCTAACTTCC 85 27 0 40.74 61.367 7.00 2.00 8.367 52 CAACTCACTGTATGAGTCTGTTGTC 73 25 0 44.00 57.993 6.00 1.00 7.007 53 CCAACTCACTGTATGAGTCTGTTGTC 72 26 0 46.15 61.357 6.00 2.00 7.357 54 AAACGCCAACTCACTGTATGA 67 21 0 42.86 58.296 3.00 2.00 2.704 55 GAAACGCCAACTCACTGTATGA 66 22 0 45.45 60.176 3.00 2.00 2.176 56 AGAAACGCCAACTCACTGTATGA 65 23 0 43.48 61.075 3.00 2.00 4.075 57 TAGAAACGCCAACTCACTGTATGA 64 24 0 41.67 61.047 3.00 2.00 5.047 58 ATAGAAACGCCAACTCACTGTATGA 63 25 0 40.00 61.238 3.00 2.00 6.238 59 GATAGAAACGCCAACTCACTGTATGA 62 26 0 42.31 62.768 3.00 2.00 8.768 60 GAAACGCCAACTCACTGTATG 66 21 0 47.62 58.296 3.00 1.00 2.704 61 AGAAACGCCAACTCACTGTATG 65 22 0 45.45 59.309 3.00 1.00 2.691 62 TAGAAACGCCAACTCACTGTATG 64 23 0 43.48 59.344 3.00 1.00 3.656 63 ATAGAAACGCCAACTCACTGTATG 63 24 0 41.67 59.623 3.00 1.00 4.377 64 GATAGAAACGCCAACTCACTGTATG 62 25 0 44.00 61.238 3.00 1.00 6.238 65 ATAGAAACGCCAACTCACTGTAT 63 23 0 39.13 57.058 3.00 2.00 5.942 66 GATAGAAACGCCAACTCACTGTAT 62 24 0 41.67 58.785 3.00 2.00 5.215 67 TGATAGAAACGCCAACTCACTGTAT 61 25 0 40.00 61.238 3.00 2.00 6.238 68 CTGATAGAAACGCCAACTCACTGTAT 60 26 0 42.31 61.990 3.00 2.00 7.990 69 GATAGAAACGCCAACTCACTGTA 62 23 0 43.48 58.464 3.00 2.00 4.536 70 TGATAGAAACGCCAACTCACTGTA 61 24 0 41.67 61.047 3.00 2.00 5.047 71 CTGATAGAAACGCCAACTCACTGTA 60 25 0 44.00 61.842 3.00 2.00 6.842 72 TGATAGAAACGCCAACTCACT 61 21 0 42.86 57.496 3.00 1.00 3.504 73 CTGATAGAAACGCCAACTCACT 60 22 0 45.45 58.536 3.00 1.00 3.464 74 CCTGATAGAAACGCCAACTCACT 59 23 0 47.83 61.863 3.00 1.00 4.863 75 ACCTGATAGAAACGCCAACTCACT 58 24 0 45.83 62.526 3.00 1.00 6.526 76 CTGATAGAAACGCCAACTCAC 60 21 0 47.62 57.496 3.00 0.00 3.504 77 CCTGATAGAAACGCCAACTCAC 59 22 0 50.00 61.033 3.00 0.00 3.033 78 ACCTGATAGAAACGCCAACTCAC 58 23 0 47.83 61.755 3.00 0.00 4.755 79 CCTGATAGAAACGCCAACTCA 59 21 0 47.62 60.256 3.00 1.00 1.256 80 ACCTGATAGAAACGCCAACTCA 58 22 0 45.45 61.033 3.00 1.00 3.033 81 GACCTGATAGAAACGCCAACTCA 57 23 0 47.83 62.713 3.00 1.00 5.713 82 CCTGATAGAAACGCCAACTC 59 20 0 50.00 57.422 2.00 0.00 2.578 83 ACCTGATAGAAACGCCAACTC 58 21 0 47.62 58.332 2.00 0.00 2.668 84 GACCTGATAGAAACGCCAACTC 57 22 0 50.00 60.137 2.00 0.00 2.137 85 TGACCTGATAGAAACGCCAACTC 56 23 0 47.83 62.713 2.00 0.00 5.713 86 ATGACCTGATAGAAACGCCAACTC 55 24 0 45.83 62.827 2.00 0.00 6.827 87 TATGACCTGATAGAAACGCCAACTC 54 25 0 44.00 62.743 4.00 0.00 7.743 88 GACCTGATAGAAACGCCAACT 57 21 0 47.62 58.332 2.00 1.00 2.668 89 TGACCTGATAGAAACGCCAACT 56 22 0 45.45 61.033 2.00 1.00 3.033 90 ATGACCTGATAGAAACGCCAACT 55 23 0 43.48 61.233 2.00 1.00 4.233 91 TATGACCTGATAGAAACGCCAACT 54 24 0 41.67 61.201 4.00 1.00 5.201 92 CTATGACCTGATAGAAACGCCAACT 53 25 0 44.00 61.959 6.00 1.00 6.959 93 GACCTGATAGAAACGCCAAC 57 20 0 50.00 57.265 2.00 0.00 2.735 94 TGACCTGATAGAAACGCCAAC 56 21 0 47.62 60.125 2.00 0.00 1.125 95 ATGACCTGATAGAAACGCCAAC 55 22 0 45.45 60.381 2.00 0.00 2.381 96 TATGACCTGATAGAAACGCCAAC 54 23 0 43.48 60.377 4.00 0.00 3.377 97 CTATGACCTGATAGAAACGCCAAC 53 24 0 45.83 61.201 6.00 0.00 5.201 98 TCTATGACCTGATAGAAACGCCAAC 52 25 0 44.00 62.743 8.00 0.00 7.743 99 TGACCTGATAGAAACGCCAA 56 20 0 45.00 59.272 2.00 0.00 0.728 100 ATGACCTGATAGAAACGCCAA 55 21 0 42.86 59.583 2.00 0.00 1.417 101 TATGACCTGATAGAAACGCCAA 54 22 0 40.91 59.609 4.00 0.00 2.391 102 CTATGACCTGATAGAAACGCCAA 53 23 0 43.48 60.495 6.00 0.00 3.495 103 TCTATGACCTGATAGAAACGCCAA 52 24 0 41.67 62.110 8.00 0.00 6.110 104 TGACCTGATAGAAACGCCA 56 19 0 47.37 57.759 2.00 0.00 3.241 105 ATGACCTGATAGAAACGCCA 55 20 0 45.00 58.173 2.00 0.00 1.827 106 TATGACCTGATAGAAACGCCA 54 21 0 42.86 58.254 4.00 0.00 2.746 107 CTATGACCTGATAGAAACGCCA 53 22 0 45.45 59.248 6.00 0.00 2.752 108 TCTATGACCTGATAGAAACGCCA 52 23 0 43.48 60.980 8.00 0.00 3.980 109 TTCTATGACCTGATAGAAACGCCA 51 24 0 41.67 62.110 10.00 0.00 6.110 110 TCTATGACCTGATAGAAACGCC 52 22 0 45.45 58.353 8.00 1.00 3.647 111 TTCTATGACCTGATAGAAACGCC 51 23 0 43.48 59.643 10.00 1.00 3.357 112 TTTCTATGACCTGATAGAAACGCC 50 24 0 41.67 60.811 12.00 1.00 4.811 113 TTTTCTATGACCTGATAGAAACGCC 49 25 0 40.00 61.874 12.00 2.00 6.874 114 TTTCTATGACCTGATAGAAACGC 50 23 0 39.13 57.615 12.00 2.00 5.385 115 CAATCAAATTTTTTCTATGACCTGAT 39 26 0 26.92 58.947 6.00 2.00 7.053 116 GCAATCAAATTTTTTCTATGACCTGAT 38 27 0 29.63 61.857 6.00 2.00 8.857 117 CAATCAAATTTTTTCTATGACCTGA 39 25 0 28.00 58.665 6.00 1.00 6.335 118 GCAATCAAATTTTTTCTATGACCTGA 38 26 0 30.77 61.716 6.00 1.00 7.716 119 CAATCAAATTTTTTCTATGACCTG 39 24 0 29.17 57.024 6.00 1.00 6.976 120 GCAATCAAATTTTTTCTATGACCTG 38 25 0 32.00 60.235 6.00 1.00 5.235 121 GCAATCAAATTTTTTCTATGACCT 38 24 0 29.17 57.882 6.00 1.00 6.118 122 CGCAATCAAATTTTTTCTATGACCT 37 25 0 32.00 61.717 6.00 1.00 6.717 123 CGCAATCAAATTTTTTCTATGACC 37 24 0 33.33 60.984 6.00 0.00 4.984 124 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 7.463 125 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 8.574 126 CGCAATCAAATTTTTTCTATGAC 37 23 0 30.43 57.905 6.00 1.00 5.095 127 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 4.458 128 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 5.211 129 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 6.843 130 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 9.235 131 CGCAATCAAATTTTTTCTATGA 37 22 0 27.27 57.077 6.00 2.00 4.923 132 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 4.207 133 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 5.202 134 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 7.653 135 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 4.923 136 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 5.544 137 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 5.323 138 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 5.202 139 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 8.397 140 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 9.507 141 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 5.439 142 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 4.023 143 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 5.202 144 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 6.204 145 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 8.199 146 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 4.533 147 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 3.033 148 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 4.199 149 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 5.202 150 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 7.226 151 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 8.382 152 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 2.909 153 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 3.626 154 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 4.596 155 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 5.502 156 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 6.694 157 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 8.652 primer3-2.3.6/test/primer_list_last/SP01-06-with-repeat2.rev0000664000077300007730000003634712231156244022362 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self lib qual- # sequence start ln N GC% Tm any end sim lity 0 GTGCTTTGCGTTTTGGAAGT 125 20 0 45.00 60.292 3.00 1.00 9.00 0.292 1 GTGCTTTGCGTTTTGGAAG 125 19 0 47.37 59.454 3.00 0.00 8.00 1.546 2 TGCTTTGCGTTTTGGAAGT 124 19 0 42.11 59.454 3.00 1.00 9.00 1.546 3 TGCTTTGCGTTTTGGAAGTTA 124 21 0 38.10 60.764 3.00 2.00 9.00 1.764 4 GCTTTGCGTTTTGGAAGTTA 123 20 0 40.00 58.097 3.00 2.00 9.00 1.903 5 GCTTTGCGTTTTGGAAGTTAG 123 21 0 42.86 59.065 3.00 0.00 9.00 1.935 6 GAGCGTGCTTTGCGTTTT 129 18 0 50.00 60.142 4.00 0.00 9.00 2.142 7 GCTTTGCGTTTTGGAAGTTAGA 123 22 0 40.91 60.748 3.00 1.00 8.00 2.748 8 AGCGTGCTTTGCGTTTTG 128 18 0 50.00 61.131 4.00 0.00 8.00 3.131 9 TGCTTTGCGTTTTGGAAG 124 18 0 44.44 58.546 3.00 0.00 8.00 3.454 10 GTGCTTTGCGTTTTGGAAGTTA 125 22 0 40.91 61.484 3.00 2.00 9.00 3.484 11 TGCTTTGCGTTTTGGAAGTTAG 124 22 0 40.91 61.593 3.00 0.00 10.00 3.593 12 GTGCTTTGCGTTTTGGAA 125 18 0 44.44 58.389 2.00 2.00 9.00 3.611 13 TGCGTTTTGGAAGTTAGACTTTG 119 23 0 39.13 60.658 6.00 2.00 9.00 3.658 14 CTTTGCGTTTTGGAAGTTAGA 122 21 0 38.10 57.272 3.00 1.00 8.00 3.728 15 TTTGCGTTTTGGAAGTTAGA 121 20 0 35.00 56.191 3.00 1.00 8.00 3.809 16 CTTTGCGTTTTGGAAGTTAGAC 122 22 0 40.91 58.127 3.00 2.00 9.00 3.873 17 GCGTTTTGGAAGTTAGACTTTG 118 22 0 40.91 58.127 6.00 2.00 9.00 3.873 18 TTTGCGTTTTGGAAGTTAGAC 121 21 0 38.10 57.124 3.00 2.00 9.00 3.876 19 CGTGCTTTGCGTTTTGGA 126 18 0 50.00 61.944 2.00 0.00 8.00 3.944 20 CGTGCTTTGCGTTTTGGAAG 126 20 0 50.00 63.967 3.00 0.00 8.00 3.967 21 GAGCGTGCTTTGCGTTTTG 129 19 0 52.63 62.977 4.00 0.00 8.00 3.977 22 CGTGCTTTGCGTTTTGGAA 126 19 0 47.37 63.187 2.00 2.00 9.00 4.187 23 TTGCGTTTTGGAAGTTAGAC 120 20 0 40.00 55.619 3.00 2.00 9.00 4.381 24 GCTTTGCGTTTTGGAAGTTAGAC 123 23 0 43.48 61.438 3.00 2.00 9.00 4.438 25 GGAGCGTGCTTTGCGTTT 130 18 0 55.56 62.465 4.00 0.00 8.00 4.465 26 CTTTGCGTTTTGGAAGTTAG 122 20 0 40.00 55.385 3.00 0.00 8.00 4.615 27 GGAGCGTGCTTTGCGTTTT 130 19 0 52.63 63.650 4.00 0.00 9.00 4.650 28 GATCGGGAGCGTGCTTTG 135 18 0 61.11 62.907 4.00 0.00 8.00 4.907 29 GTGCTTTGCGTTTTGGAAGTTAG 125 23 0 43.48 62.256 3.00 0.00 10.00 5.256 30 GCTTTGCGTTTTGGAAGT 123 18 0 44.44 56.483 3.00 1.00 9.00 5.517 31 AGCGTGCTTTGCGTTTTGG 128 19 0 52.63 64.584 4.00 0.00 9.00 5.584 32 CGTGCTTTGCGTTTTGGAAGT 126 21 0 47.62 64.590 3.00 1.00 9.00 5.590 33 TTGCGTTTTGGAAGTTAGACTTTG 120 24 0 37.50 61.761 6.00 2.00 9.00 5.761 34 GCGTGCTTTGCGTTTTGG 127 18 0 55.56 63.814 2.00 0.00 9.00 5.814 35 TGCTTTGCGTTTTGGAAGTTAGA 124 23 0 39.13 63.165 3.00 1.00 9.00 6.165 36 GGAGCGTGCTTTGCGTTTTG 130 20 0 55.00 66.221 4.00 0.00 8.00 6.221 37 GAGCGTGCTTTGCGTTTTGG 129 20 0 55.00 66.221 4.00 0.00 9.00 6.221 38 AGCGTGCTTTGCGTTTTGGA 128 20 0 50.00 66.221 4.00 0.00 8.00 6.221 39 TTGCGTTTTGGAAGTTAGA 120 19 0 36.84 54.570 3.00 1.00 8.00 6.430 40 CTTTGACAACAGACTCATACAGTGA 101 25 0 40.00 58.507 5.00 2.00 10.00 6.493 41 TTTGACAACAGACTCATACAGTGA 100 24 0 37.50 57.479 5.00 2.00 9.00 6.521 42 GCGTGCTTTGCGTTTTGGAA 127 20 0 50.00 66.535 2.00 2.00 9.00 6.535 43 GCGTGCTTTGCGTTTTGGA 127 19 0 52.63 65.553 2.00 0.00 8.00 6.553 44 CGTTTTGGAAGTTAGACTTTG 117 21 0 38.10 54.425 6.00 2.00 9.00 6.575 45 ACTTTGACAACAGACTCATACAGTGA 102 26 0 38.46 59.320 5.00 2.00 10.00 6.680 46 GGGAGCGTGCTTTGCGTT 131 18 0 61.11 64.759 4.00 0.00 8.00 6.759 47 GGGAGCGTGCTTTGCGTTTT 131 20 0 55.00 66.761 4.00 0.00 9.00 6.761 48 ATCGGGAGCGTGCTTTGC 134 18 0 61.11 64.764 4.00 2.00 7.00 6.764 49 GGGAGCGTGCTTTGCGTTT 131 19 0 57.89 65.814 4.00 0.00 8.00 6.814 50 CTTTGCGTTTTGGAAGTTA 122 19 0 36.84 54.178 3.00 2.00 9.00 6.822 51 TTTGCGTTTTGGAAGTTAG 121 19 0 36.84 54.178 3.00 0.00 8.00 6.822 52 TGCGTTTTGGAAGTTAGAC 119 19 0 42.11 53.939 3.00 2.00 9.00 7.061 53 TTGACAACAGACTCATACAGTGA 99 23 0 39.13 55.925 5.00 2.00 9.00 7.075 54 GATCGGGAGCGTGCTTTGC 135 19 0 63.16 66.488 4.00 2.00 8.00 7.488 55 GTGCTTTGCGTTTTGGAAGTTAGA 125 24 0 41.67 63.758 3.00 1.00 9.00 7.758 56 TGCTTTGCGTTTTGGAAGTTAGAC 124 24 0 41.67 63.758 3.00 2.00 10.00 7.758 57 TTTGCGTTTTGGAAGTTAGACTTTG 121 25 0 36.00 62.766 6.00 2.00 9.00 7.766 58 GACTTTGACAACAGACTCATACAGTGA 103 27 0 40.74 60.988 5.00 2.00 10.00 7.988 59 AGCGTGCTTTGCGTTTTGGAA 128 21 0 47.62 67.128 4.00 2.00 9.00 8.128 60 GCGTGCTTTGCGTTTTGGAAG 127 21 0 52.38 67.128 3.00 0.00 8.00 8.128 61 AGACTTTGACAACAGACTCATACAG 104 25 0 40.00 56.820 7.00 1.00 9.00 8.180 62 AGACTTTGACAACAGACTCATACA 104 24 0 37.50 55.732 7.00 0.00 10.00 8.268 63 GACTTTGACAACAGACTCATACAG 103 24 0 41.67 55.732 5.00 1.00 9.00 8.268 64 CGTGCTTTGCGTTTTGGAAGTTA 126 23 0 43.48 65.376 3.00 2.00 9.00 8.376 65 GACTTTGACAACAGACTCATACA 103 23 0 39.13 54.538 5.00 0.00 10.00 8.462 66 TTAGACTTTGACAACAGACTCATACA 106 26 0 34.62 57.298 7.00 1.00 10.00 8.702 67 GAGCGTGCTTTGCGTTTTGGA 129 21 0 52.38 67.764 4.00 0.00 8.00 8.764 68 TTAGACTTTGACAACAGACTCATACAG 106 27 0 37.04 58.234 7.00 1.00 9.00 8.766 69 GTTAGACTTTGACAACAGACTCATACA 107 27 0 37.04 58.107 7.00 1.00 10.00 8.893 70 TAGACTTTGACAACAGACTCATACAG 105 26 0 38.46 56.940 7.00 1.00 9.00 9.060 71 TAGACTTTGACAACAGACTCATACA 105 25 0 36.00 55.897 7.00 0.00 10.00 9.103 72 TTTGCGTTTTGGAAGTTA 121 18 0 33.33 52.848 3.00 2.00 9.00 9.152 73 TGCGTTTTGGAAGTTAGA 119 18 0 38.89 52.751 3.00 1.00 8.00 9.249 74 ACTTTGACAACAGACTCATACAG 102 23 0 39.13 53.733 5.00 1.00 9.00 9.267 75 GTGCTTTGCGTTTTGGAAGTTAGAC 125 25 0 44.00 64.312 3.00 2.00 10.00 9.312 76 CTTTGACAACAGACTCATACAG 101 22 0 40.91 52.603 5.00 1.00 9.00 9.397 77 CTTTGCGTTTTGGAAGTTAGACTTTG 122 26 0 38.46 63.414 6.00 2.00 9.00 9.414 78 ATCGGGAGCGTGCTTTGCGT 134 20 0 60.00 69.470 4.00 0.00 7.00 9.470 79 ACTTTGACAACAGACTCATACA 102 22 0 36.36 52.399 5.00 0.00 10.00 9.601 80 GCGTGCTTTGCGTTTTGGAAGT 127 22 0 50.00 67.608 3.00 1.00 9.00 9.608 81 AGACTTTGACAACAGACTCA 104 20 0 40.00 50.384 7.00 1.00 9.00 9.616 82 TTGCGTTTTGGAAGTTAG 120 18 0 38.89 52.384 3.00 0.00 8.00 9.616 83 AGCGTGCTTTGCGTTTTGGAAG 128 22 0 50.00 67.671 4.00 0.00 8.00 9.671 84 AGACTTTGACAACAGACTCAT 104 21 0 38.10 51.260 7.00 2.00 9.00 9.740 85 CGGGAGCGTGCTTTGCGTTT 132 20 0 60.00 69.763 4.00 0.00 8.00 9.763 86 CTTTGACAACAGACTCATACA 101 21 0 38.10 51.152 5.00 0.00 10.00 9.848 87 TTTGACAACAGACTCATACAG 100 21 0 38.10 51.152 5.00 1.00 9.00 9.848 88 CGTGCTTTGCGTTTTGGAAGTTAG 126 24 0 45.83 65.955 3.00 0.00 10.00 9.955 89 CGGGAGCGTGCTTTGCGTT 132 19 0 63.16 68.983 4.00 0.00 8.00 9.983 90 ATCGGGAGCGTGCTTTGCG 134 19 0 63.16 69.025 4.00 2.00 8.00 10.025 91 GGGAGCGTGCTTTGCGTTTTG 131 21 0 57.14 69.108 4.00 0.00 8.00 10.108 92 GGAGCGTGCTTTGCGTTTTGG 130 21 0 57.14 69.108 4.00 0.00 9.00 10.108 93 CGGGAGCGTGCTTTGCGT 132 18 0 66.67 68.116 4.00 0.00 7.00 10.116 94 AGACTTTGACAACAGACTCATAC 104 23 0 39.13 52.792 7.00 0.00 9.00 10.208 95 GACTTTGACAACAGACTCAT 103 20 0 40.00 49.674 5.00 2.00 9.00 10.326 96 TTAGACTTTGACAACAGACTCATAC 106 25 0 36.00 54.653 7.00 1.00 9.00 10.347 97 TAGACTTTGACAACAGACTCAT 105 22 0 36.36 51.625 7.00 2.00 9.00 10.375 98 AGACTTTGACAACAGACTCATA 104 22 0 36.36 51.625 7.00 2.00 8.00 10.375 99 TTTGACAACAGACTCATACA 100 20 0 35.00 49.540 5.00 0.00 10.00 10.460 100 TCGGGAGCGTGCTTTGCGTT 133 20 0 60.00 70.467 4.00 0.00 8.00 10.467 101 GATCGGGAGCGTGCTTTGCG 135 20 0 65.00 70.520 4.00 2.00 9.00 10.520 102 GAGCGTGCTTTGCGTTTTGGAA 129 22 0 50.00 68.571 4.00 2.00 9.00 10.571 103 GACTTTGACAACAGACTCATAC 103 22 0 40.91 51.414 5.00 0.00 9.00 10.586 104 TCGGGAGCGTGCTTTGCGT 133 19 0 63.16 69.707 4.00 0.00 7.00 10.707 105 GTTTTGGAAGTTAGACTTTG 116 20 0 35.00 49.271 6.00 2.00 9.00 10.729 106 GACTTTGACAACAGACTCATA 103 21 0 38.10 50.125 5.00 2.00 8.00 10.875 107 TAGACTTTGACAACAGACTCATAC 105 24 0 37.50 53.072 7.00 0.00 9.00 10.928 108 TTGACAACAGACTCATACAG 99 20 0 40.00 49.008 5.00 1.00 9.00 10.992 109 TAGACTTTGACAACAGACTCATA 105 23 0 34.78 51.962 7.00 2.00 8.00 11.038 110 AGCGTGCTTTGCGTTTTGGAAGT 128 23 0 47.83 68.114 4.00 1.00 9.00 11.114 111 ATCGGGAGCGTGCTTTGCGTT 134 21 0 57.14 70.204 4.00 0.00 8.00 11.204 112 TCGGGAGCGTGCTTTGCG 133 18 0 66.67 69.247 4.00 2.00 7.00 11.247 113 GCGTTTTGGAAGTTAGAC 118 18 0 44.44 50.670 3.00 2.00 9.00 11.330 114 CGGGAGCGTGCTTTGCGTTTT 132 21 0 57.14 70.468 4.00 0.00 9.00 11.468 115 GATCGGGAGCGTGCTTTGCGT 135 21 0 61.90 70.899 4.00 0.00 8.00 11.899 116 ACTTTGACAACAGACTCATAC 102 21 0 38.10 49.101 5.00 0.00 9.00 11.899 117 GAGCGTGCTTTGCGTTTTGGAAG 129 23 0 52.17 69.042 4.00 0.00 9.00 12.042 118 GCGTGCTTTGCGTTTTGGAAGTTA 127 24 0 45.83 68.126 3.00 2.00 9.00 12.126 119 TCGGGAGCGTGCTTTGCGTTT 133 21 0 57.14 71.153 4.00 0.00 9.00 12.153 120 CGTGCTTTGCGTTTTGGAAGTTAGA 126 25 0 44.00 67.289 3.00 1.00 9.00 12.289 121 ACTTTGACAACAGACTCATA 102 20 0 35.00 47.663 5.00 2.00 8.00 12.337 122 CTTTGACAACAGACTCATAC 101 20 0 40.00 47.663 5.00 0.00 9.00 12.337 123 GACTTTGACAACAGACTCA 103 19 0 42.11 48.639 5.00 1.00 9.00 12.361 124 GGAGCGTGCTTTGCGTTTTGGA 130 22 0 54.55 70.490 4.00 0.00 9.00 12.490 125 ATCGGGAGCGTGCTTTGCGTTT 134 22 0 54.55 70.870 4.00 0.00 9.00 12.870 126 GCTTTGCGTTTTGGAAGTTAGACTTTG 123 27 0 40.74 66.026 6.00 2.00 9.00 13.026 127 TTTTGGAAGTTAGACTTTG 115 19 0 31.58 47.928 6.00 2.00 9.00 13.072 128 GAGCGTGCTTTGCGTTTTGGAAGT 129 24 0 50.00 69.429 4.00 1.00 9.00 13.429 129 GATCGGGAGCGTGCTTTGCGTT 135 22 0 59.09 71.545 4.00 0.00 9.00 13.545 130 AGCGTGCTTTGCGTTTTGGAAGTTA 128 25 0 44.00 68.576 4.00 2.00 9.00 13.576 131 GCGTGCTTTGCGTTTTGGAAGTTAG 127 25 0 48.00 68.576 3.00 0.00 10.00 13.576 132 GGGAGCGTGCTTTGCGTTTTGG 131 22 0 59.09 71.695 4.00 0.00 9.00 13.695 133 CGTGCTTTGCGTTTTGGAAGTTAGAC 126 26 0 46.15 67.709 3.00 2.00 10.00 13.709 134 TCGGGAGCGTGCTTTGCGTTTT 133 22 0 54.55 71.774 4.00 0.00 9.00 13.774 135 TTGACAACAGACTCATACA 99 19 0 36.84 47.159 5.00 0.00 10.00 13.841 136 ACTTTGACAACAGACTCAT 102 19 0 36.84 47.081 5.00 2.00 9.00 13.919 137 GGAGCGTGCTTTGCGTTTTGGAA 130 23 0 52.17 71.131 4.00 2.00 9.00 14.131 138 ATCGGGAGCGTGCTTTGCGTTTT 134 23 0 52.17 71.476 4.00 0.00 9.00 14.476 139 CGGGAGCGTGCTTTGCGTTTTG 132 22 0 59.09 72.594 4.00 0.00 8.00 14.594 140 CTTTGACAACAGACTCATA 101 19 0 36.84 46.089 5.00 2.00 8.00 14.911 141 AGCGTGCTTTGCGTTTTGGAAGTTAG 128 26 0 46.15 68.993 4.00 0.00 10.00 14.993 142 GATCGGGAGCGTGCTTTGCGTTT 135 23 0 56.52 72.132 4.00 0.00 10.00 15.132 143 TTTGACAACAGACTCATAC 100 19 0 36.84 45.794 5.00 0.00 9.00 15.206 144 GGAGCGTGCTTTGCGTTTTGGAAG 130 24 0 54.17 71.481 4.00 0.00 10.00 15.481 145 GAGCGTGCTTTGCGTTTTGGAAGTTA 129 26 0 46.15 69.786 4.00 2.00 9.00 15.786 146 GCGTGCTTTGCGTTTTGGAAGTTAGA 127 26 0 46.15 69.786 3.00 1.00 9.00 15.786 147 GGGAGCGTGCTTTGCGTTTTGGA 131 23 0 56.52 72.940 4.00 0.00 9.00 15.940 148 ACTTTGACAACAGACTCA 102 18 0 38.89 45.825 5.00 1.00 9.00 16.175 149 TTTGGAAGTTAGACTTTG 114 18 0 33.33 45.649 6.00 2.00 8.00 16.351 150 CTTTGACAACAGACTCAT 101 18 0 38.89 45.402 5.00 2.00 9.00 16.598 151 GATCGGGAGCGTGCTTTGCGTTTT 135 24 0 54.17 72.668 4.00 0.00 9.00 16.668 152 GGAGCGTGCTTTGCGTTTTGGAAGT 130 25 0 52.00 71.774 4.00 1.00 9.00 16.774 153 TCGGGAGCGTGCTTTGCGTTTTG 133 23 0 56.52 73.813 4.00 0.00 8.00 16.813 154 GCGTGCTTTGCGTTTTGGAAGTTAGAC 127 27 0 48.15 70.114 3.00 2.00 10.00 17.114 155 GAGCGTGCTTTGCGTTTTGGAAGTTAG 129 27 0 48.15 70.152 4.00 0.00 10.00 17.152 156 AGCGTGCTTTGCGTTTTGGAAGTTAGA 128 27 0 44.44 70.152 4.00 1.00 9.00 17.152 157 ATCGGGAGCGTGCTTTGCGTTTTG 134 24 0 54.17 73.434 4.00 0.00 8.00 17.434 158 GGGAGCGTGCTTTGCGTTTTGGAA 131 24 0 54.17 73.443 4.00 2.00 9.00 17.443 159 CGGGAGCGTGCTTTGCGTTTTGG 132 23 0 60.87 74.875 4.00 0.00 9.00 17.875 160 TTTGACAACAGACTCATA 100 18 0 33.33 44.031 5.00 2.00 8.00 17.969 161 GGGAGCGTGCTTTGCGTTTTGGAAG 131 25 0 56.00 73.692 4.00 0.00 10.00 18.692 162 TTGACAACAGACTCATAC 99 18 0 38.89 43.056 5.00 0.00 9.00 18.944 163 GGAGCGTGCTTTGCGTTTTGGAAGTTA 130 27 0 48.15 71.956 4.00 2.00 9.00 18.956 164 GATCGGGAGCGTGCTTTGCGTTTTG 135 25 0 56.00 74.552 4.00 0.00 8.00 19.552 165 GGGAGCGTGCTTTGCGTTTTGGAAGT 131 26 0 53.85 73.906 4.00 1.00 9.00 19.906 166 TCGGGAGCGTGCTTTGCGTTTTGG 133 24 0 58.33 75.980 4.00 0.00 9.00 19.980 167 CGGGAGCGTGCTTTGCGTTTTGGA 132 24 0 58.33 75.980 4.00 0.00 9.00 19.980 168 ATCGGGAGCGTGCTTTGCGTTTTGG 134 25 0 56.00 75.528 4.00 0.00 9.00 20.528 169 CGGGAGCGTGCTTTGCGTTTTGGAA 132 25 0 56.00 76.331 4.00 2.00 9.00 21.331 170 TCGGGAGCGTGCTTTGCGTTTTGGA 133 25 0 56.00 77.032 4.00 0.00 9.00 22.032 171 CGGGAGCGTGCTTTGCGTTTTGGAAG 132 26 0 57.69 76.468 4.00 0.00 10.00 22.468 172 GATCGGGAGCGTGCTTTGCGTTTTGG 135 26 0 57.69 76.548 4.00 0.00 9.00 22.548 173 ATCGGGAGCGTGCTTTGCGTTTTGGA 134 26 0 53.85 76.548 4.00 0.00 9.00 22.548 174 TCGGGAGCGTGCTTTGCGTTTTGGAA 133 26 0 53.85 77.333 4.00 2.00 9.00 23.333 175 CGGGAGCGTGCTTTGCGTTTTGGAAGT 132 27 0 55.56 76.595 4.00 1.00 9.00 23.595 176 ATCGGGAGCGTGCTTTGCGTTTTGGAA 134 27 0 51.85 76.856 4.00 2.00 9.00 23.856 177 TCGGGAGCGTGCTTTGCGTTTTGGAAG 133 27 0 55.56 77.432 4.00 0.00 10.00 24.432 178 GATCGGGAGCGTGCTTTGCGTTTTGGA 135 27 0 55.56 77.522 4.00 0.00 9.00 24.522 primer3-2.3.6/test/primer_list_last/targets_test.for0000664000077300007730000000065012231156244021631 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TCCTACAGCTGTGGGAAAATG 0 21 0 47.62 60.118 12.00 0.00 1.118 1 CCTACAGCTGTGGGAAAATG 1 20 0 50.00 58.228 10.00 0.00 1.772 2 TCCTACAGCTGTGGGAAAAT 0 20 0 45.00 57.268 12.00 2.00 2.732 primer3-2.3.6/test/primer_list_last/included_test.rev0000664000077300007730000006241112231156244021760 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 CACAACAGACACACACACACAA 186 22 0 45.45 59.121 1.00 0.00 2.879 1 CAACAGACACACACACACAACA 184 22 0 45.45 59.121 1.00 0.00 2.879 2 CACACACACAACACACAGTCAA 175 22 0 45.45 59.121 3.00 0.00 2.879 3 ACACAACAGACACACACACACAA 187 23 0 43.48 60.064 1.00 0.00 3.064 4 CACAACAGACACACACACACAAC 186 23 0 47.83 60.064 1.00 0.00 3.064 5 ACAACAGACACACACACACAACA 185 23 0 43.48 60.064 1.00 0.00 3.064 6 CAACAGACACACACACACAACAC 184 23 0 47.83 60.064 1.00 0.00 3.064 7 AACAGACACACACACACAACACA 183 23 0 43.48 60.064 1.00 0.00 3.064 8 ACACACACACAACACACAGTCAA 176 23 0 43.48 60.064 3.00 0.00 3.064 9 CACACACACAACACACAGTCAAG 175 23 0 47.83 60.208 3.00 0.00 3.208 10 CACACACAACACACAGTCAAGAA 173 23 0 43.48 59.744 3.00 0.00 3.256 11 AGACACACACACACAACACACAG 180 23 0 47.83 59.650 1.00 1.00 3.350 12 ACACAACAGACACACACACACA 187 22 0 45.45 58.514 1.00 0.00 3.486 13 ACAGACACACACACACAACACA 182 22 0 45.45 58.514 1.00 0.00 3.486 14 CAGACACACACACACAACACAC 181 22 0 50.00 58.514 1.00 0.00 3.486 15 AGACACACACACACAACACACA 180 22 0 45.45 58.514 1.00 0.00 3.486 16 GACACACACACACAACACACAG 179 22 0 50.00 58.514 1.00 1.00 3.486 17 CACACACACACAACACACAGTC 177 22 0 50.00 58.514 3.00 1.00 3.486 18 ACACACACACAACACACAGTCA 176 22 0 45.45 58.514 3.00 1.00 3.486 19 ACAGACACACACACACAACACAC 182 23 0 47.83 59.499 1.00 0.00 3.501 20 GACACACACACACAACACACAGT 179 23 0 47.83 59.499 3.00 3.00 3.501 21 ACACACACACACAACACACAGTC 178 23 0 47.83 59.499 3.00 1.00 3.501 22 CACAACAGACACACACACACA 186 21 0 47.62 57.434 1.00 0.00 3.566 23 CAGACACACACACACAACACA 181 21 0 47.62 57.434 1.00 0.00 3.566 24 CACACACACAACACACAGTCA 175 21 0 47.62 57.434 3.00 1.00 3.566 25 TGCACAATACCACACATGTTT 121 21 0 38.10 57.391 6.00 6.00 3.609 26 GACACACACACACAACACACA 179 21 0 47.62 57.244 1.00 0.00 3.756 27 CACACACAACACACAGTCAAGA 173 22 0 45.45 58.217 3.00 2.00 3.783 28 ACACACACAACACACAGTCAAGA 174 23 0 43.48 59.188 3.00 2.00 3.812 29 CACACAACACACAGTCAAGAAGC 171 23 0 47.83 60.832 3.00 2.00 3.832 30 CACAACACACAGTCAAGAAGCT 169 22 0 45.45 58.075 4.00 4.00 3.925 31 CACAGTCAAGAAGCTACATCCA 162 22 0 45.45 58.036 4.00 0.00 3.964 32 ACACAACACACAGTCAAGAAGCT 170 23 0 43.48 58.981 4.00 4.00 4.019 33 TACACAACAGACACACACACACAA 188 24 0 41.67 60.073 2.00 0.00 4.073 34 CACACAGTCAAGAAGCTACATCC 164 23 0 47.83 58.925 4.00 0.00 4.075 35 ACACAGTCAAGAAGCTACATCCA 163 23 0 43.48 58.925 4.00 0.00 4.075 36 TGCACAATACCACACATGTTTAA 121 23 0 34.78 58.925 6.00 4.00 4.075 37 ACACAACACACAGTCAAGAAGC 170 22 0 45.45 57.918 3.00 2.00 4.082 38 ATGCACAATACCACACATGTTT 122 22 0 36.36 57.811 6.00 6.00 4.189 39 ACACACAGTCAAGAAGCTACATCC 165 24 0 45.83 59.748 4.00 0.00 4.252 40 TGCACAATACCACACATGTTTAAC 121 24 0 37.50 59.733 6.00 5.00 4.267 41 GCACAATACCACACATGTTTAACA 120 24 0 37.50 59.733 7.00 7.00 4.267 42 TACACAACAGACACACACACACA 188 23 0 43.48 58.590 2.00 0.00 4.410 43 TGCACAATACCACACATGTTTA 121 22 0 36.36 57.504 6.00 4.00 4.496 44 GCTACATCCATACACAACACACA 150 23 0 43.48 58.499 2.00 0.00 4.501 45 AGCTACATCCATACACAACACACA 151 24 0 41.67 59.493 4.00 0.00 4.507 46 AGACACACACACACAACACACAGT 180 24 0 45.83 60.539 3.00 3.00 4.539 47 CACAGTCAAGAAGCTACATCCAT 162 23 0 43.48 58.408 4.00 2.00 4.592 48 ACACACACAACACACAGTCAAGAA 174 24 0 41.67 60.599 3.00 0.00 4.599 49 ACACACACACACAACACACAGT 178 22 0 45.45 57.352 3.00 3.00 4.648 50 CACAACACACATGTATATGCACAA 138 24 0 37.50 59.345 6.00 2.00 4.655 51 CAGACACACACACACAACACACA 181 23 0 47.83 61.701 1.00 0.00 4.701 52 CACACACACACAACACACAGTCA 177 23 0 47.83 61.701 3.00 1.00 4.701 53 CACACACAACACACAGTCAAGAAG 173 24 0 45.83 60.730 3.00 0.00 4.730 54 ACACAGTCAAGAAGCTACATCCAT 163 24 0 41.67 59.238 4.00 2.00 4.762 55 ATGCACAATACCACACATGTTTAA 122 24 0 33.33 59.233 6.00 4.00 4.767 56 CACAACACACAGTCAAGAAGCTA 169 23 0 43.48 58.160 4.00 2.00 4.840 57 CAACACACAGTCAAGAAGCTACA 167 23 0 43.48 58.160 4.00 2.00 4.840 58 GAAGCTACATCCATACACAACACA 153 24 0 41.67 59.091 4.00 0.00 4.909 59 ACACACACAACACACAGTCAAG 174 22 0 45.45 57.090 3.00 0.00 4.910 60 ACACAACAGACACACACACACAAC 187 24 0 45.83 60.929 1.00 0.00 4.929 61 ACAACAGACACACACACACAACAC 185 24 0 45.83 60.929 1.00 0.00 4.929 62 AACAGACACACACACACAACACAC 183 24 0 45.83 60.929 1.00 0.00 4.929 63 ACACAACACACAGTCAAGAAGCTA 170 24 0 41.67 59.030 4.00 2.00 4.970 64 CACAACACACAGTCAAGAAGCTAC 169 24 0 45.83 59.030 4.00 4.00 4.970 65 ACAACACACAGTCAAGAAGCTACA 168 24 0 41.67 59.030 4.00 2.00 4.970 66 AGAAGCTACATCCATACACAACACA 154 25 0 40.00 60.002 4.00 0.00 5.002 67 ATGCACAATACCACACATGTTTAAC 122 25 0 36.00 59.991 6.00 5.00 5.009 68 ATACACAACAGACACACACACACA 189 24 0 41.67 58.961 2.00 0.00 5.039 69 ACACACACACAACACACAGTCAAG 176 24 0 45.83 61.059 3.00 0.00 5.059 70 CACAACACACATGTATATGCACA 138 23 0 39.13 57.923 6.00 4.00 5.077 71 TATGCACAATACCACACATGTTT 123 23 0 34.78 57.903 6.00 6.00 5.097 72 ATGCACAATACCACACATGTTTA 122 23 0 34.78 57.903 6.00 4.00 5.097 73 GAAGCTACATCCATACACAACACAC 153 25 0 44.00 59.880 4.00 0.00 5.120 74 ACACAACACACATGTATATGCACAA 139 25 0 36.00 60.151 6.00 2.00 5.151 75 GCTACATCCATACACAACACACAT 150 24 0 41.67 58.842 2.00 2.00 5.158 76 ACACACAACACACAGTCAAGAAG 172 23 0 43.48 57.837 3.00 0.00 5.163 77 ACACAACACACAGTCAAGAAGCTAC 170 25 0 44.00 59.835 4.00 4.00 5.165 78 TCAAGAAGCTACATCCATACACAA 157 24 0 37.50 58.834 4.00 0.00 5.166 79 ACACAACACACATGTATATGCACA 139 24 0 37.50 58.828 6.00 4.00 5.172 80 AGCTACATCCATACACAACACACAT 151 25 0 40.00 59.772 4.00 2.00 5.228 81 CAACACACAGTCAAGAAGCTACATC 167 25 0 44.00 60.272 4.00 2.00 5.272 82 ATACACAACAGACACACACACACAA 189 25 0 40.00 60.344 2.00 0.00 5.344 83 ACATCCATACACAACACACATGT 147 23 0 39.13 57.646 6.00 6.00 5.354 84 CACAACACACATGTATATGCACAAT 138 25 0 36.00 59.637 6.00 2.00 5.363 85 GTCAAGAAGCTACATCCATACACAA 158 25 0 40.00 59.615 4.00 0.00 5.385 86 TCAAGAAGCTACATCCATACACAAC 157 25 0 40.00 59.615 4.00 0.00 5.385 87 CAAGAAGCTACATCCATACACAACA 156 25 0 40.00 60.458 4.00 0.00 5.458 88 CAACACACAGTCAAGAAGCTACAT 167 24 0 41.67 58.520 4.00 4.00 5.480 89 GACACACACACACAACACACAGTC 179 24 0 50.00 61.504 3.00 1.00 5.504 90 CACAGTCAAGAAGCTACATCCATA 162 24 0 41.67 58.476 4.00 2.00 5.524 91 CAGTCAAGAAGCTACATCCATACA 160 24 0 41.67 58.476 4.00 0.00 5.524 92 TCAAGAAGCTACATCCATACACA 157 23 0 39.13 57.467 4.00 0.00 5.533 93 GCACAATACCACACATGTTTAACAG 120 25 0 40.00 60.599 7.00 5.00 5.599 94 ACACACAACACACAGTCAAGAAGC 172 24 0 45.83 61.613 3.00 2.00 5.613 95 CACACAGTCAAGAAGCTACATCCA 164 24 0 45.83 61.619 4.00 0.00 5.619 96 GTCAAGAAGCTACATCCATACACA 158 24 0 41.67 58.339 4.00 0.00 5.661 97 ACAACACACAGTCAAGAAGCTACAT 168 25 0 40.00 59.333 4.00 4.00 5.667 98 CACACATGTATATGCACAATACCA 133 24 0 37.50 58.315 6.00 4.00 5.685 99 CACATGTATATGCACAATACCACA 131 24 0 37.50 58.315 6.00 2.00 5.685 100 CATGTATATGCACAATACCACACA 129 24 0 37.50 58.315 6.00 0.00 5.685 101 TGTATATGCACAATACCACACATG 127 24 0 37.50 58.315 6.00 4.00 5.685 102 ACACAGTCAAGAAGCTACATCCATA 163 25 0 40.00 59.274 4.00 2.00 5.726 103 CACAGTCAAGAAGCTACATCCATAC 162 25 0 44.00 59.274 4.00 0.00 5.726 104 ACAGTCAAGAAGCTACATCCATACA 161 25 0 40.00 59.274 4.00 0.00 5.726 105 CAGTCAAGAAGCTACATCCATACAC 160 25 0 44.00 59.274 4.00 0.00 5.726 106 AGTCAAGAAGCTACATCCATACACA 159 25 0 40.00 59.274 4.00 0.00 5.726 107 CACACAACACACAGTCAAGAAGCT 171 24 0 45.83 61.730 4.00 4.00 5.730 108 TATGCACAATACCACACATGTTTAA 123 25 0 32.00 59.268 6.00 4.00 5.732 109 ATATGCACAATACCACACATGTTT 124 24 0 33.33 58.262 6.00 6.00 5.738 110 AAGCTACATCCATACACAACACACA 152 25 0 40.00 60.742 4.00 0.00 5.742 111 AAGCTACATCCATACACAACACA 152 23 0 39.13 57.257 4.00 0.00 5.743 112 AAGCTACATCCATACACAACACAC 152 24 0 41.67 58.137 4.00 0.00 5.863 113 CCATACACAACACACATGTATATGC 143 25 0 40.00 59.136 6.00 4.00 5.864 114 ACACACATGTATATGCACAATACCA 134 25 0 36.00 59.136 6.00 4.00 5.864 115 CACACATGTATATGCACAATACCAC 133 25 0 40.00 59.136 6.00 2.00 5.864 116 ACACATGTATATGCACAATACCACA 132 25 0 36.00 59.136 6.00 2.00 5.864 117 CACATGTATATGCACAATACCACAC 131 25 0 40.00 59.136 6.00 0.00 5.864 118 ACATGTATATGCACAATACCACACA 130 25 0 36.00 59.136 6.00 0.00 5.864 119 TGTATATGCACAATACCACACATGT 127 25 0 36.00 59.136 6.00 6.00 5.864 120 TACACAACAGACACACACACACAAC 188 25 0 44.00 60.904 2.00 0.00 5.904 121 GCACAATACCACACATGTTTAAC 120 23 0 39.13 57.060 6.00 5.00 5.940 122 AACACACAGTCAAGAAGCTACATCC 166 25 0 44.00 60.962 4.00 0.00 5.962 123 CAAGAAGCTACATCCATACACAA 156 23 0 39.13 57.036 4.00 0.00 5.964 124 TATACACAACAGACACACACACACA 190 25 0 40.00 59.013 4.00 0.00 5.987 125 TATGCACAATACCACACATGTTTAAC 123 26 0 34.62 60.001 6.00 5.00 6.001 126 TATGCACAATACCACACATGTTTA 123 24 0 33.33 57.988 6.00 4.00 6.012 127 ACACAGTCAAGAAGCTACATCCATAC 163 26 0 42.31 60.018 4.00 0.00 6.018 128 ACAGTCAAGAAGCTACATCCATACAC 161 26 0 42.31 60.018 4.00 0.00 6.018 129 CACAATACCACACATGTTTAACAGA 119 25 0 36.00 58.912 7.00 3.00 6.088 130 CAAGAAGCTACATCCATACACAAC 156 24 0 41.67 57.904 4.00 0.00 6.096 131 AAGAAGCTACATCCATACACAACA 155 24 0 37.50 57.904 4.00 0.00 6.096 132 ACACACATGTATATGCACAATACCAC 134 26 0 38.46 59.900 6.00 2.00 6.100 133 ACACATGTATATGCACAATACCACAC 132 26 0 38.46 59.900 6.00 0.00 6.100 134 CTACATCCATACACAACACACATG 149 24 0 41.67 57.896 4.00 4.00 6.104 135 TACACAACACACATGTATATGCACA 140 25 0 36.00 58.882 6.00 4.00 6.118 136 CACACACACAACACACAGTCAAGA 175 24 0 45.83 62.141 3.00 2.00 6.141 137 TACACAACACACATGTATATGCACAA 140 26 0 34.62 60.156 6.00 2.00 6.156 138 CTACATCCATACACAACACACATGT 149 25 0 40.00 58.761 6.00 6.00 6.239 139 TACATCCATACACAACACACATGT 148 24 0 37.50 57.747 6.00 6.00 6.253 140 ACATCCATACACAACACACATGTA 147 24 0 37.50 57.747 6.00 4.00 6.253 141 AAGAAGCTACATCCATACACAACAC 155 25 0 40.00 58.712 4.00 0.00 6.288 142 CACAATACCACACATGTTTAACAGAC 119 26 0 38.46 59.680 7.00 1.00 6.320 143 CACAACACACATGTATATGCACAATA 138 26 0 34.62 59.659 6.00 4.00 6.341 144 TATACACAACAGACACACACACACAA 190 26 0 38.46 60.342 4.00 0.00 6.342 145 GTCAAGAAGCTACATCCATACACAAC 158 26 0 42.31 60.343 4.00 0.00 6.343 146 CAACACACATGTATATGCACAATACC 136 26 0 38.46 60.346 6.00 4.00 6.346 147 AACACACATGTATATGCACAATACCA 135 26 0 34.62 60.346 6.00 4.00 6.346 148 TGTATATGCACAATACCACACATGTT 127 26 0 34.62 60.346 6.00 4.00 6.346 149 CATGTATATGCACAATACCACACAT 129 25 0 36.00 58.650 6.00 2.00 6.350 150 ATGTATATGCACAATACCACACATG 128 25 0 36.00 58.650 6.00 4.00 6.350 151 AACACACAGTCAAGAAGCTACATC 166 24 0 41.67 57.626 4.00 2.00 6.374 152 ACACAACACACATGTATATGCACAAT 139 26 0 34.62 60.393 6.00 2.00 6.393 153 AGTCAAGAAGCTACATCCATACACAA 159 26 0 38.46 60.455 4.00 0.00 6.455 154 ATATGCACAATACCACACATGTTTAA 124 26 0 30.77 59.536 6.00 4.00 6.464 155 GCTACATCCATACACAACACACATG 150 25 0 44.00 61.472 4.00 4.00 6.472 156 ACACACACAACACACAGTCAAGAAG 174 25 0 44.00 61.508 3.00 0.00 6.508 157 ACAGACACACACACACAACACACA 182 24 0 45.83 62.520 1.00 0.00 6.520 158 ACACACACACACAACACACAGTCA 178 24 0 45.83 62.520 3.00 1.00 6.520 159 ACATGTATATGCACAATACCACACAT 130 26 0 34.62 59.420 6.00 2.00 6.580 160 ATGTATATGCACAATACCACACATGT 128 26 0 34.62 59.420 6.00 6.00 6.580 161 AGAAGCTACATCCATACACAACAC 154 24 0 41.67 57.400 4.00 0.00 6.600 162 CATACACAACACACATGTATATGCA 142 25 0 36.00 58.378 6.00 6.00 6.622 163 CAGACACACACACACAACACACAG 181 24 0 50.00 62.641 1.00 1.00 6.641 164 TATATGCACAATACCACACATGTTT 125 25 0 32.00 58.331 6.00 6.00 6.669 165 ATATGCACAATACCACACATGTTTA 124 25 0 32.00 58.331 6.00 4.00 6.669 166 ATATACACAACAGACACACACACACA 191 26 0 38.46 59.329 4.00 0.00 6.671 167 CACACAACACACAGTCAAGAAGCTA 171 25 0 44.00 61.678 4.00 2.00 6.678 168 CACAACACACAGTCAAGAAGCTACA 169 25 0 44.00 61.678 4.00 2.00 6.678 169 AGAAGCTACATCCATACACAACACAC 154 26 0 42.31 60.727 4.00 0.00 6.727 170 CATCCATACACAACACACATGTAT 146 24 0 37.50 57.241 6.00 6.00 6.759 171 CACACACACACAACATACACTCTAGA 241 26 0 42.31 59.211 6.00 6.00 6.789 172 CACACACACAACATACACTCTAGACA 239 26 0 42.31 59.211 6.00 2.00 6.789 173 CACACACAACATACACTCTAGACACA 237 26 0 42.31 59.211 6.00 0.00 6.789 174 CACACAGTCAAGAAGCTACATCCAT 164 25 0 44.00 61.791 4.00 2.00 6.791 175 CATACACAACACACATGTATATGCAC 142 26 0 38.46 59.190 6.00 4.00 6.810 176 ATACACAACACACATGTATATGCACA 141 26 0 34.62 59.190 6.00 4.00 6.810 177 TCCATACACAACACACATGTATATGC 144 26 0 38.46 60.811 6.00 4.00 6.811 178 CACAATACCACACATGTTTAACAG 119 24 0 37.50 57.140 7.00 5.00 6.860 179 ACATCCATACACAACACACATGTAT 147 25 0 36.00 58.124 6.00 6.00 6.876 180 ACACACACACACAACATACACTCT 242 24 0 41.67 57.096 2.00 1.00 6.904 181 GTATATGCACAATACCACACATGTTT 126 26 0 34.62 59.095 6.00 6.00 6.905 182 TCTACACACACACACAACATACACTC 245 26 0 42.31 59.077 2.00 0.00 6.923 183 AAGCTACATCCATACACAACACACAT 152 26 0 38.46 60.948 4.00 2.00 6.948 184 TATATGCACAATACCACACATGTT 125 24 0 33.33 57.002 6.00 4.00 6.998 185 TCTACACACACACACAACATACACTCT 245 27 0 40.74 60.001 2.00 1.00 7.001 186 ACACACACACACAACATACACTCTAGA 242 27 0 40.74 60.001 6.00 6.00 7.001 187 CACACACACACAACATACACTCTAGAC 241 27 0 44.44 60.001 6.00 4.00 7.001 188 ACACACACACAACATACACTCTAGACA 240 27 0 40.74 60.001 6.00 2.00 7.001 189 CACACACACAACATACACTCTAGACAC 239 27 0 44.44 60.001 6.00 0.00 7.001 190 ACACACACAACATACACTCTAGACACA 238 27 0 40.74 60.001 6.00 0.00 7.001 191 ACAACACACAGTCAAGAAGCTACATC 168 26 0 42.31 61.001 4.00 2.00 7.001 192 ATACACAACAGACACACACACACAAC 189 26 0 42.31 61.121 2.00 0.00 7.121 193 CACACACAACATACACTCTAGACACAG 237 27 0 44.44 60.122 6.00 1.00 7.122 194 CATGTATATGCACAATACCACACATG 129 26 0 38.46 61.147 6.00 4.00 7.147 195 AACACACATGTATATGCACAATACC 135 25 0 36.00 57.851 6.00 4.00 7.149 196 GTATATGCACAATACCACACATGTT 126 25 0 36.00 57.851 6.00 4.00 7.149 197 CAAGAAGCTACATCCATACACAACAC 156 26 0 42.31 61.157 4.00 0.00 7.157 198 AAGAAGCTACATCCATACACAACACA 155 26 0 38.46 61.157 4.00 0.00 7.157 199 TACATCCATACACAACACACATGTA 148 25 0 36.00 57.841 6.00 4.00 7.159 200 CTACATCCATACACAACACACATGTA 149 26 0 38.46 58.815 6.00 4.00 7.185 201 ATATGCACAATACCACACATGTTTAAC 124 27 0 33.33 60.227 6.00 5.00 7.227 202 CATCCATACACAACACACATGTATATG 146 27 0 37.04 60.230 6.00 6.00 7.230 203 AACACACGTGTATATACACAACAGACA 202 27 0 37.04 59.748 12.00 1.00 7.252 204 TGCACAATACCACACATGTTTAACA 121 25 0 36.00 62.264 7.00 7.00 7.264 205 ACACACAGTCAAGAAGCTACATCCA 165 25 0 44.00 62.322 4.00 0.00 7.322 206 CACACGTGTATATACACAACAGACA 200 25 0 40.00 57.664 12.00 1.00 7.336 207 CACGTGTATATACACAACAGACACA 198 25 0 40.00 57.664 12.00 1.00 7.336 208 CGTGTATATACACAACAGACACACA 196 25 0 40.00 57.664 12.00 0.00 7.336 209 ATACACAACACACATGTATATGCACAA 141 27 0 33.33 60.389 6.00 2.00 7.389 210 TACACAACACACATGTATATGCACAAT 140 27 0 33.33 60.389 6.00 2.00 7.389 211 ACACAACACACATGTATATGCACAATA 139 27 0 33.33 60.389 6.00 4.00 7.389 212 CACAACACACATGTATATGCACAATAC 138 27 0 37.04 60.389 6.00 6.00 7.389 213 AGACACACACACACAACACACAGTC 180 25 0 48.00 62.414 3.00 1.00 7.414 214 TATATGCACAATACCACACATGTTTAA 125 27 0 29.63 59.560 6.00 4.00 7.440 215 ACACACAACACACAGTCAAGAAGCT 172 25 0 44.00 62.443 4.00 4.00 7.443 216 GATCTACACACACACACAACATACA 247 25 0 40.00 57.548 4.00 0.00 7.452 217 ACACACGTGTATATACACAACAGACA 201 26 0 38.46 58.508 12.00 1.00 7.492 218 CACACGTGTATATACACAACAGACAC 200 26 0 42.31 58.508 12.00 3.00 7.492 219 ACACGTGTATATACACAACAGACACA 199 26 0 38.46 58.508 12.00 1.00 7.492 220 CACGTGTATATACACAACAGACACAC 198 26 0 42.31 58.508 12.00 1.00 7.492 221 ACGTGTATATACACAACAGACACACA 197 26 0 38.46 58.508 12.00 0.00 7.492 222 CGTGTATATACACAACAGACACACAC 196 26 0 42.31 58.508 12.00 0.00 7.492 223 ATGTATATGCACAATACCACACATGTT 128 27 0 33.33 60.562 6.00 4.00 7.562 224 GATCTACACACACACACAACATACAC 247 26 0 42.31 58.431 4.00 0.00 7.569 225 ATATACACAACAGACACACACACACAA 191 27 0 37.04 60.577 4.00 0.00 7.577 226 TATATGCACAATACCACACATGTTTA 125 26 0 30.77 58.395 6.00 4.00 7.605 227 GATCTACACACACACACAACATACACT 247 27 0 40.74 59.376 4.00 1.00 7.624 228 ATCTACACACACACACAACATACACTC 246 27 0 40.74 59.376 2.00 0.00 7.624 229 CACACACACACAACATACACTCTAG 241 25 0 44.00 57.370 4.00 4.00 7.630 230 TGTATATACACAACAGACACACACACA 194 27 0 37.04 59.363 10.00 0.00 7.637 231 TATATACACAACAGACACACACACACA 192 27 0 37.04 59.363 6.00 0.00 7.637 232 CATCCATACACAACACACATGTATA 146 25 0 36.00 57.352 6.00 4.00 7.648 233 TCCATACACAACACACATGTATATG 144 25 0 36.00 57.352 6.00 6.00 7.648 234 CCATACACAACACACATGTATATGCA 143 26 0 38.46 61.654 6.00 6.00 7.654 235 CACACATGTATATGCACAATACCACA 133 26 0 38.46 61.654 6.00 2.00 7.654 236 CACATGTATATGCACAATACCACACA 131 26 0 38.46 61.654 6.00 0.00 7.654 237 ACACACGTGTATATACACAACAGACAC 201 27 0 40.74 59.293 12.00 3.00 7.707 238 ACACGTGTATATACACAACAGACACAC 199 27 0 40.74 59.293 12.00 1.00 7.707 239 ACGTGTATATACACAACAGACACACAC 197 27 0 40.74 59.293 12.00 0.00 7.707 240 CACACAGTCAAGAAGCTACATCCATA 164 26 0 42.31 61.740 4.00 2.00 7.740 241 CACAGTCAAGAAGCTACATCCATACA 162 26 0 42.31 61.740 4.00 0.00 7.740 242 CAGTCAAGAAGCTACATCCATACACA 160 26 0 42.31 61.740 4.00 0.00 7.740 243 CTACACACACACACAACATACACTCT 244 26 0 42.31 58.251 2.00 1.00 7.749 244 ACACACACACACAACATACACTCTAG 242 26 0 42.31 58.251 4.00 4.00 7.749 245 TCTACACACACACACAACATACACT 245 25 0 40.00 57.217 2.00 1.00 7.783 246 CTACACACACACACAACATACACTC 244 25 0 44.00 57.217 2.00 0.00 7.783 247 TACACACACACACAACATACACTCT 243 25 0 40.00 57.217 2.00 1.00 7.783 248 ACACACACACACAACATACACTCTA 242 25 0 40.00 57.217 3.00 3.00 7.783 249 TACATCCATACACAACACACATGTAT 148 26 0 34.62 58.200 6.00 6.00 7.800 250 ACATCCATACACAACACACATGTATA 147 26 0 34.62 58.200 6.00 4.00 7.800 251 CACAACACACAGTCAAGAAGCTACAT 169 26 0 42.31 61.844 4.00 4.00 7.844 252 GAACACACGTGTATATACACAACAGA 203 26 0 38.46 58.139 12.00 4.00 7.861 253 GTATATGCACAATACCACACATGTTTA 126 27 0 33.33 59.133 6.00 4.00 7.867 254 CTACATCCATACACAACACACATGTAT 149 27 0 37.04 59.113 6.00 6.00 7.887 255 ACACACACACAACACACAGTCAAGA 176 25 0 44.00 62.889 3.00 2.00 7.889 256 ACAACACACATGTATATGCACAATA 137 25 0 32.00 57.073 6.00 4.00 7.927 257 CAACACACATGTATATGCACAATAC 136 25 0 36.00 57.073 6.00 6.00 7.927 258 AGAACACACGTGTATATACACAACAGA 204 27 0 37.04 59.049 12.00 4.00 7.951 259 ATCCATACACAACACACATGTATATGC 145 27 0 37.04 61.010 6.00 4.00 8.010 260 ACAACACACATGTATATGCACAATACC 137 27 0 37.04 61.034 6.00 4.00 8.034 261 AACACACATGTATATGCACAATACCAC 135 27 0 37.04 61.034 6.00 2.00 8.034 262 CACACGTGTATATACACAACAGACACA 200 27 0 40.74 61.042 12.00 1.00 8.042 263 CACGTGTATATACACAACAGACACACA 198 27 0 40.74 61.042 12.00 0.00 8.042 264 CGTGTATATACACAACAGACACACACA 196 27 0 40.74 61.042 12.00 0.00 8.042 265 TCAAGAAGCTACATCCATACACAACA 157 26 0 38.46 62.056 4.00 0.00 8.056 266 GAACACACGTGTATATACACAACAGAC 203 27 0 40.74 58.926 12.00 2.00 8.074 267 ACAACACACATGTATATGCACAATAC 137 26 0 34.62 57.913 6.00 6.00 8.087 268 TATACACAACAGACACACACACACAAC 190 27 0 40.74 61.092 4.00 0.00 8.092 269 AGTCAAGAAGCTACATCCATACACAAC 159 27 0 40.74 61.126 4.00 0.00 8.126 270 GCTACATCCATACACAACACACATGT 150 26 0 42.31 62.163 6.00 6.00 8.163 271 GCACAATACCACACATGTTTAACAGA 120 26 0 38.46 62.185 7.00 3.00 8.185 272 AGCTACATCCATACACAACACACATG 151 26 0 42.31 62.266 4.00 4.00 8.266 273 CATCCATACACAACACACATGTATAT 146 26 0 34.62 57.730 6.00 6.00 8.270 274 ATCCATACACAACACACATGTATATG 145 26 0 34.62 57.730 6.00 6.00 8.270 275 ACACACAACACACAGTCAAGAAGCTA 172 26 0 42.31 62.367 4.00 2.00 8.367 276 CACACAACACACAGTCAAGAAGCTAC 171 26 0 46.15 62.367 4.00 4.00 8.367 277 ACACAACACACAGTCAAGAAGCTACA 170 26 0 42.31 62.367 4.00 2.00 8.367 278 GAAGCTACATCCATACACAACACACA 153 26 0 42.31 62.368 4.00 0.00 8.368 279 ATCTACACACACACACAACATACACT 246 26 0 38.46 57.620 2.00 1.00 8.380 280 ATGCACAATACCACACATGTTTAACA 122 26 0 34.62 62.397 7.00 7.00 8.397 281 TGTATATGCACAATACCACACATGTTT 127 27 0 33.33 61.445 6.00 6.00 8.445 282 ACACACAGTCAAGAAGCTACATCCAT 165 26 0 42.31 62.454 4.00 2.00 8.454 283 ACATCCATACACAACACACATGTATAT 147 27 0 33.33 58.526 6.00 6.00 8.474 284 ACACACAACATACACTCTAGACACAG 236 26 0 42.31 57.508 6.00 1.00 8.492 285 AGAACACACGTGTATATACACAACAG 204 26 0 38.46 57.375 12.00 6.00 8.625 286 ACACACACACAACATACACTCTAGAC 240 26 0 42.31 57.364 6.00 4.00 8.636 287 ACACACACAACATACACTCTAGACAC 238 26 0 42.31 57.364 6.00 0.00 8.636 288 TACACACACACACAACATACACTCTA 243 26 0 38.46 57.330 3.00 3.00 8.670 289 CTACACACACACACAACATACACTCTA 244 27 0 40.74 58.323 3.00 3.00 8.677 290 TACACACACACACAACATACACTCTAG 243 27 0 40.74 58.323 4.00 4.00 8.677 291 CATACACAACACACATGTATATGCACA 142 27 0 37.04 61.688 6.00 4.00 8.688 292 TACATCCATACACAACACACATGTATA 148 27 0 33.33 58.271 6.00 4.00 8.729 293 AACACACGTGTATATACACAACAGAC 202 26 0 38.46 57.237 12.00 2.00 8.763 294 AAGAAGCTACATCCATACACAACACAC 155 27 0 40.74 61.811 4.00 0.00 8.811 295 CACATGTATATGCACAATACCACACAT 131 27 0 37.04 61.814 6.00 2.00 8.814 296 ACATGTATATGCACAATACCACACATG 130 27 0 37.04 61.814 6.00 4.00 8.814 297 CATGTATATGCACAATACCACACATGT 129 27 0 37.04 61.814 6.00 6.00 8.814 298 CACAATACCACACATGTTTAACAGACA 119 27 0 37.04 62.089 7.00 3.00 9.089 299 GCTACATCCATACACAACACACATGTA 150 27 0 40.74 62.100 6.00 4.00 9.100 300 CCATACACAACACACATGTATATGCAC 143 27 0 40.74 62.312 6.00 4.00 9.312 301 ACACACATGTATATGCACAATACCACA 134 27 0 37.04 62.312 6.00 2.00 9.312 302 CACACATGTATATGCACAATACCACAC 133 27 0 40.74 62.312 6.00 0.00 9.312 303 ACACATGTATATGCACAATACCACACA 132 27 0 37.04 62.312 6.00 0.00 9.312 304 TATGCACAATACCACACATGTTTAACA 123 27 0 33.33 62.329 7.00 7.00 9.329 305 ACACACAGTCAAGAAGCTACATCCATA 165 27 0 40.74 62.383 4.00 2.00 9.383 306 CACACAGTCAAGAAGCTACATCCATAC 164 27 0 44.44 62.383 4.00 0.00 9.383 307 ACACAGTCAAGAAGCTACATCCATACA 163 27 0 40.74 62.383 4.00 0.00 9.383 308 CACAGTCAAGAAGCTACATCCATACAC 162 27 0 44.44 62.383 4.00 0.00 9.383 309 ACAGTCAAGAAGCTACATCCATACACA 161 27 0 40.74 62.383 4.00 0.00 9.383 310 GTGTATATACACAACAGACACACACAC 195 27 0 40.74 57.575 12.00 0.00 9.425 311 GTATATACACAACAGACACACACACAC 193 27 0 40.74 57.575 8.00 0.00 9.425 312 GAAGCTACATCCATACACAACACACAT 153 27 0 40.74 62.494 4.00 2.00 9.494 313 ACACAACACACAGTCAAGAAGCTACAT 170 27 0 40.74 62.495 4.00 4.00 9.495 314 TAGAACACACGTGTATATACACAACAG 205 27 0 37.04 57.472 12.00 6.00 9.528 315 GTCAAGAAGCTACATCCATACACAACA 158 27 0 40.74 62.685 4.00 0.00 9.685 316 TCAAGAAGCTACATCCATACACAACAC 157 27 0 40.74 62.685 4.00 0.00 9.685 317 CAACACACATGTATATGCACAATACCA 136 27 0 37.04 62.716 6.00 4.00 9.716 318 CAGTCAAGAAGCTACATCCATACACAA 160 27 0 40.74 62.778 4.00 0.00 9.778 319 GCACAATACCACACATGTTTAACAGAC 120 27 0 40.74 62.806 7.00 1.00 9.806 320 AGCTACATCCATACACAACACACATGT 151 27 0 40.74 62.902 6.00 6.00 9.902 primer3-2.3.6/test/primer_list_last/SP01-21.txt.rev0000664000077300007730000006227112231156244020657 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 CACAACAGACACACACACACAA 166 22 0 45.45 59.121 1.00 0.00 2.879 1 CAACAGACACACACACACAACA 164 22 0 45.45 59.121 1.00 0.00 2.879 2 CACACACACAACACACAGTCAA 155 22 0 45.45 59.121 3.00 0.00 2.879 3 ACACAACAGACACACACACACAA 167 23 0 43.48 60.064 1.00 0.00 3.064 4 CACAACAGACACACACACACAAC 166 23 0 47.83 60.064 1.00 0.00 3.064 5 ACAACAGACACACACACACAACA 165 23 0 43.48 60.064 1.00 0.00 3.064 6 CAACAGACACACACACACAACAC 164 23 0 47.83 60.064 1.00 0.00 3.064 7 AACAGACACACACACACAACACA 163 23 0 43.48 60.064 1.00 0.00 3.064 8 ACACACACACAACACACAGTCAA 156 23 0 43.48 60.064 3.00 0.00 3.064 9 CACACACACAACACACAGTCAAG 155 23 0 47.83 60.208 3.00 0.00 3.208 10 CACACACAACACACAGTCAAGAA 153 23 0 43.48 59.744 3.00 0.00 3.256 11 AGACACACACACACAACACACAG 160 23 0 47.83 59.650 1.00 1.00 3.350 12 ACACAACAGACACACACACACA 167 22 0 45.45 58.514 1.00 0.00 3.486 13 ACAGACACACACACACAACACA 162 22 0 45.45 58.514 1.00 0.00 3.486 14 CAGACACACACACACAACACAC 161 22 0 50.00 58.514 1.00 0.00 3.486 15 AGACACACACACACAACACACA 160 22 0 45.45 58.514 1.00 0.00 3.486 16 GACACACACACACAACACACAG 159 22 0 50.00 58.514 1.00 1.00 3.486 17 CACACACACACAACACACAGTC 157 22 0 50.00 58.514 3.00 1.00 3.486 18 ACACACACACAACACACAGTCA 156 22 0 45.45 58.514 3.00 1.00 3.486 19 ACAGACACACACACACAACACAC 162 23 0 47.83 59.499 1.00 0.00 3.501 20 GACACACACACACAACACACAGT 159 23 0 47.83 59.499 3.00 3.00 3.501 21 ACACACACACACAACACACAGTC 158 23 0 47.83 59.499 3.00 1.00 3.501 22 CACAACAGACACACACACACA 166 21 0 47.62 57.434 1.00 0.00 3.566 23 CAGACACACACACACAACACA 161 21 0 47.62 57.434 1.00 0.00 3.566 24 CACACACACAACACACAGTCA 155 21 0 47.62 57.434 3.00 1.00 3.566 25 TGCACAATACCACACATGTTT 101 21 0 38.10 57.391 6.00 6.00 3.609 26 GACACACACACACAACACACA 159 21 0 47.62 57.244 1.00 0.00 3.756 27 CACACACAACACACAGTCAAGA 153 22 0 45.45 58.217 3.00 2.00 3.783 28 ACACACACAACACACAGTCAAGA 154 23 0 43.48 59.188 3.00 2.00 3.812 29 CACACAACACACAGTCAAGAAGC 151 23 0 47.83 60.832 3.00 2.00 3.832 30 CACAACACACAGTCAAGAAGCT 149 22 0 45.45 58.075 4.00 4.00 3.925 31 CACAGTCAAGAAGCTACATCCA 142 22 0 45.45 58.036 4.00 0.00 3.964 32 ACACAACACACAGTCAAGAAGCT 150 23 0 43.48 58.981 4.00 4.00 4.019 33 TACACAACAGACACACACACACAA 168 24 0 41.67 60.073 2.00 0.00 4.073 34 CACACAGTCAAGAAGCTACATCC 144 23 0 47.83 58.925 4.00 0.00 4.075 35 ACACAGTCAAGAAGCTACATCCA 143 23 0 43.48 58.925 4.00 0.00 4.075 36 TGCACAATACCACACATGTTTAA 101 23 0 34.78 58.925 6.00 4.00 4.075 37 ACACAACACACAGTCAAGAAGC 150 22 0 45.45 57.918 3.00 2.00 4.082 38 ATGCACAATACCACACATGTTT 102 22 0 36.36 57.811 6.00 6.00 4.189 39 ACACACAGTCAAGAAGCTACATCC 145 24 0 45.83 59.748 4.00 0.00 4.252 40 TGCACAATACCACACATGTTTAAC 101 24 0 37.50 59.733 6.00 5.00 4.267 41 GCACAATACCACACATGTTTAACA 100 24 0 37.50 59.733 7.00 7.00 4.267 42 TACACAACAGACACACACACACA 168 23 0 43.48 58.590 2.00 0.00 4.410 43 TGCACAATACCACACATGTTTA 101 22 0 36.36 57.504 6.00 4.00 4.496 44 GCTACATCCATACACAACACACA 130 23 0 43.48 58.499 2.00 0.00 4.501 45 AGCTACATCCATACACAACACACA 131 24 0 41.67 59.493 4.00 0.00 4.507 46 AGACACACACACACAACACACAGT 160 24 0 45.83 60.539 3.00 3.00 4.539 47 CACAGTCAAGAAGCTACATCCAT 142 23 0 43.48 58.408 4.00 2.00 4.592 48 ACACACACAACACACAGTCAAGAA 154 24 0 41.67 60.599 3.00 0.00 4.599 49 ACACACACACACAACACACAGT 158 22 0 45.45 57.352 3.00 3.00 4.648 50 CACAACACACATGTATATGCACAA 118 24 0 37.50 59.345 6.00 2.00 4.655 51 CAGACACACACACACAACACACA 161 23 0 47.83 61.701 1.00 0.00 4.701 52 CACACACACACAACACACAGTCA 157 23 0 47.83 61.701 3.00 1.00 4.701 53 CACACACAACACACAGTCAAGAAG 153 24 0 45.83 60.730 3.00 0.00 4.730 54 ACACAGTCAAGAAGCTACATCCAT 143 24 0 41.67 59.238 4.00 2.00 4.762 55 ATGCACAATACCACACATGTTTAA 102 24 0 33.33 59.233 6.00 4.00 4.767 56 CACAACACACAGTCAAGAAGCTA 149 23 0 43.48 58.160 4.00 2.00 4.840 57 CAACACACAGTCAAGAAGCTACA 147 23 0 43.48 58.160 4.00 2.00 4.840 58 GAAGCTACATCCATACACAACACA 133 24 0 41.67 59.091 4.00 0.00 4.909 59 ACACACACAACACACAGTCAAG 154 22 0 45.45 57.090 3.00 0.00 4.910 60 ACACAACAGACACACACACACAAC 167 24 0 45.83 60.929 1.00 0.00 4.929 61 ACAACAGACACACACACACAACAC 165 24 0 45.83 60.929 1.00 0.00 4.929 62 AACAGACACACACACACAACACAC 163 24 0 45.83 60.929 1.00 0.00 4.929 63 ACACAACACACAGTCAAGAAGCTA 150 24 0 41.67 59.030 4.00 2.00 4.970 64 CACAACACACAGTCAAGAAGCTAC 149 24 0 45.83 59.030 4.00 4.00 4.970 65 ACAACACACAGTCAAGAAGCTACA 148 24 0 41.67 59.030 4.00 2.00 4.970 66 AGAAGCTACATCCATACACAACACA 134 25 0 40.00 60.002 4.00 0.00 5.002 67 ATGCACAATACCACACATGTTTAAC 102 25 0 36.00 59.991 6.00 5.00 5.009 68 ATACACAACAGACACACACACACA 169 24 0 41.67 58.961 2.00 0.00 5.039 69 ACACACACACAACACACAGTCAAG 156 24 0 45.83 61.059 3.00 0.00 5.059 70 CACAACACACATGTATATGCACA 118 23 0 39.13 57.923 6.00 4.00 5.077 71 TATGCACAATACCACACATGTTT 103 23 0 34.78 57.903 6.00 6.00 5.097 72 ATGCACAATACCACACATGTTTA 102 23 0 34.78 57.903 6.00 4.00 5.097 73 GAAGCTACATCCATACACAACACAC 133 25 0 44.00 59.880 4.00 0.00 5.120 74 ACACAACACACATGTATATGCACAA 119 25 0 36.00 60.151 6.00 2.00 5.151 75 GCTACATCCATACACAACACACAT 130 24 0 41.67 58.842 2.00 2.00 5.158 76 ACACACAACACACAGTCAAGAAG 152 23 0 43.48 57.837 3.00 0.00 5.163 77 ACACAACACACAGTCAAGAAGCTAC 150 25 0 44.00 59.835 4.00 4.00 5.165 78 TCAAGAAGCTACATCCATACACAA 137 24 0 37.50 58.834 4.00 0.00 5.166 79 ACACAACACACATGTATATGCACA 119 24 0 37.50 58.828 6.00 4.00 5.172 80 AGCTACATCCATACACAACACACAT 131 25 0 40.00 59.772 4.00 2.00 5.228 81 CAACACACAGTCAAGAAGCTACATC 147 25 0 44.00 60.272 4.00 2.00 5.272 82 ATACACAACAGACACACACACACAA 169 25 0 40.00 60.344 2.00 0.00 5.344 83 ACATCCATACACAACACACATGT 127 23 0 39.13 57.646 6.00 6.00 5.354 84 CACAACACACATGTATATGCACAAT 118 25 0 36.00 59.637 6.00 2.00 5.363 85 GTCAAGAAGCTACATCCATACACAA 138 25 0 40.00 59.615 4.00 0.00 5.385 86 TCAAGAAGCTACATCCATACACAAC 137 25 0 40.00 59.615 4.00 0.00 5.385 87 CAAGAAGCTACATCCATACACAACA 136 25 0 40.00 60.458 4.00 0.00 5.458 88 CAACACACAGTCAAGAAGCTACAT 147 24 0 41.67 58.520 4.00 4.00 5.480 89 GACACACACACACAACACACAGTC 159 24 0 50.00 61.504 3.00 1.00 5.504 90 CACAGTCAAGAAGCTACATCCATA 142 24 0 41.67 58.476 4.00 2.00 5.524 91 CAGTCAAGAAGCTACATCCATACA 140 24 0 41.67 58.476 4.00 0.00 5.524 92 TCAAGAAGCTACATCCATACACA 137 23 0 39.13 57.467 4.00 0.00 5.533 93 GCACAATACCACACATGTTTAACAG 100 25 0 40.00 60.599 7.00 5.00 5.599 94 ACACACAACACACAGTCAAGAAGC 152 24 0 45.83 61.613 3.00 2.00 5.613 95 CACACAGTCAAGAAGCTACATCCA 144 24 0 45.83 61.619 4.00 0.00 5.619 96 GTCAAGAAGCTACATCCATACACA 138 24 0 41.67 58.339 4.00 0.00 5.661 97 ACAACACACAGTCAAGAAGCTACAT 148 25 0 40.00 59.333 4.00 4.00 5.667 98 CACACATGTATATGCACAATACCA 113 24 0 37.50 58.315 6.00 4.00 5.685 99 CACATGTATATGCACAATACCACA 111 24 0 37.50 58.315 6.00 2.00 5.685 100 CATGTATATGCACAATACCACACA 109 24 0 37.50 58.315 6.00 0.00 5.685 101 TGTATATGCACAATACCACACATG 107 24 0 37.50 58.315 6.00 4.00 5.685 102 ACACAGTCAAGAAGCTACATCCATA 143 25 0 40.00 59.274 4.00 2.00 5.726 103 CACAGTCAAGAAGCTACATCCATAC 142 25 0 44.00 59.274 4.00 0.00 5.726 104 ACAGTCAAGAAGCTACATCCATACA 141 25 0 40.00 59.274 4.00 0.00 5.726 105 CAGTCAAGAAGCTACATCCATACAC 140 25 0 44.00 59.274 4.00 0.00 5.726 106 AGTCAAGAAGCTACATCCATACACA 139 25 0 40.00 59.274 4.00 0.00 5.726 107 CACACAACACACAGTCAAGAAGCT 151 24 0 45.83 61.730 4.00 4.00 5.730 108 TATGCACAATACCACACATGTTTAA 103 25 0 32.00 59.268 6.00 4.00 5.732 109 ATATGCACAATACCACACATGTTT 104 24 0 33.33 58.262 6.00 6.00 5.738 110 AAGCTACATCCATACACAACACACA 132 25 0 40.00 60.742 4.00 0.00 5.742 111 AAGCTACATCCATACACAACACA 132 23 0 39.13 57.257 4.00 0.00 5.743 112 AAGCTACATCCATACACAACACAC 132 24 0 41.67 58.137 4.00 0.00 5.863 113 CCATACACAACACACATGTATATGC 123 25 0 40.00 59.136 6.00 4.00 5.864 114 ACACACATGTATATGCACAATACCA 114 25 0 36.00 59.136 6.00 4.00 5.864 115 CACACATGTATATGCACAATACCAC 113 25 0 40.00 59.136 6.00 2.00 5.864 116 ACACATGTATATGCACAATACCACA 112 25 0 36.00 59.136 6.00 2.00 5.864 117 CACATGTATATGCACAATACCACAC 111 25 0 40.00 59.136 6.00 0.00 5.864 118 ACATGTATATGCACAATACCACACA 110 25 0 36.00 59.136 6.00 0.00 5.864 119 TGTATATGCACAATACCACACATGT 107 25 0 36.00 59.136 6.00 6.00 5.864 120 TACACAACAGACACACACACACAAC 168 25 0 44.00 60.904 2.00 0.00 5.904 121 GCACAATACCACACATGTTTAAC 100 23 0 39.13 57.060 6.00 5.00 5.940 122 AACACACAGTCAAGAAGCTACATCC 146 25 0 44.00 60.962 4.00 0.00 5.962 123 CAAGAAGCTACATCCATACACAA 136 23 0 39.13 57.036 4.00 0.00 5.964 124 TATACACAACAGACACACACACACA 170 25 0 40.00 59.013 4.00 0.00 5.987 125 TATGCACAATACCACACATGTTTAAC 103 26 0 34.62 60.001 6.00 5.00 6.001 126 TATGCACAATACCACACATGTTTA 103 24 0 33.33 57.988 6.00 4.00 6.012 127 ACACAGTCAAGAAGCTACATCCATAC 143 26 0 42.31 60.018 4.00 0.00 6.018 128 ACAGTCAAGAAGCTACATCCATACAC 141 26 0 42.31 60.018 4.00 0.00 6.018 129 CACAATACCACACATGTTTAACAGA 99 25 0 36.00 58.912 7.00 3.00 6.088 130 CAAGAAGCTACATCCATACACAAC 136 24 0 41.67 57.904 4.00 0.00 6.096 131 AAGAAGCTACATCCATACACAACA 135 24 0 37.50 57.904 4.00 0.00 6.096 132 ACACACATGTATATGCACAATACCAC 114 26 0 38.46 59.900 6.00 2.00 6.100 133 ACACATGTATATGCACAATACCACAC 112 26 0 38.46 59.900 6.00 0.00 6.100 134 CTACATCCATACACAACACACATG 129 24 0 41.67 57.896 4.00 4.00 6.104 135 TACACAACACACATGTATATGCACA 120 25 0 36.00 58.882 6.00 4.00 6.118 136 CACACACACAACACACAGTCAAGA 155 24 0 45.83 62.141 3.00 2.00 6.141 137 TACACAACACACATGTATATGCACAA 120 26 0 34.62 60.156 6.00 2.00 6.156 138 CTACATCCATACACAACACACATGT 129 25 0 40.00 58.761 6.00 6.00 6.239 139 TACATCCATACACAACACACATGT 128 24 0 37.50 57.747 6.00 6.00 6.253 140 ACATCCATACACAACACACATGTA 127 24 0 37.50 57.747 6.00 4.00 6.253 141 AAGAAGCTACATCCATACACAACAC 135 25 0 40.00 58.712 4.00 0.00 6.288 142 CACAATACCACACATGTTTAACAGAC 99 26 0 38.46 59.680 7.00 1.00 6.320 143 CACAACACACATGTATATGCACAATA 118 26 0 34.62 59.659 6.00 4.00 6.341 144 TATACACAACAGACACACACACACAA 170 26 0 38.46 60.342 4.00 0.00 6.342 145 GTCAAGAAGCTACATCCATACACAAC 138 26 0 42.31 60.343 4.00 0.00 6.343 146 CAACACACATGTATATGCACAATACC 116 26 0 38.46 60.346 6.00 4.00 6.346 147 AACACACATGTATATGCACAATACCA 115 26 0 34.62 60.346 6.00 4.00 6.346 148 TGTATATGCACAATACCACACATGTT 107 26 0 34.62 60.346 6.00 4.00 6.346 149 CATGTATATGCACAATACCACACAT 109 25 0 36.00 58.650 6.00 2.00 6.350 150 ATGTATATGCACAATACCACACATG 108 25 0 36.00 58.650 6.00 4.00 6.350 151 AACACACAGTCAAGAAGCTACATC 146 24 0 41.67 57.626 4.00 2.00 6.374 152 ACACAACACACATGTATATGCACAAT 119 26 0 34.62 60.393 6.00 2.00 6.393 153 AGTCAAGAAGCTACATCCATACACAA 139 26 0 38.46 60.455 4.00 0.00 6.455 154 ATATGCACAATACCACACATGTTTAA 104 26 0 30.77 59.536 6.00 4.00 6.464 155 GCTACATCCATACACAACACACATG 130 25 0 44.00 61.472 4.00 4.00 6.472 156 ACACACACAACACACAGTCAAGAAG 154 25 0 44.00 61.508 3.00 0.00 6.508 157 ACAGACACACACACACAACACACA 162 24 0 45.83 62.520 1.00 0.00 6.520 158 ACACACACACACAACACACAGTCA 158 24 0 45.83 62.520 3.00 1.00 6.520 159 ACATGTATATGCACAATACCACACAT 110 26 0 34.62 59.420 6.00 2.00 6.580 160 ATGTATATGCACAATACCACACATGT 108 26 0 34.62 59.420 6.00 6.00 6.580 161 AGAAGCTACATCCATACACAACAC 134 24 0 41.67 57.400 4.00 0.00 6.600 162 CATACACAACACACATGTATATGCA 122 25 0 36.00 58.378 6.00 6.00 6.622 163 CAGACACACACACACAACACACAG 161 24 0 50.00 62.641 1.00 1.00 6.641 164 TATATGCACAATACCACACATGTTT 105 25 0 32.00 58.331 6.00 6.00 6.669 165 ATATGCACAATACCACACATGTTTA 104 25 0 32.00 58.331 6.00 4.00 6.669 166 ATATACACAACAGACACACACACACA 171 26 0 38.46 59.329 4.00 0.00 6.671 167 CACACAACACACAGTCAAGAAGCTA 151 25 0 44.00 61.678 4.00 2.00 6.678 168 CACAACACACAGTCAAGAAGCTACA 149 25 0 44.00 61.678 4.00 2.00 6.678 169 AGAAGCTACATCCATACACAACACAC 134 26 0 42.31 60.727 4.00 0.00 6.727 170 CATCCATACACAACACACATGTAT 126 24 0 37.50 57.241 6.00 6.00 6.759 171 CACACACACACAACATACACTCTAGA 221 26 0 42.31 59.211 6.00 6.00 6.789 172 CACACACACAACATACACTCTAGACA 219 26 0 42.31 59.211 6.00 2.00 6.789 173 CACACACAACATACACTCTAGACACA 217 26 0 42.31 59.211 6.00 0.00 6.789 174 CACACAGTCAAGAAGCTACATCCAT 144 25 0 44.00 61.791 4.00 2.00 6.791 175 CATACACAACACACATGTATATGCAC 122 26 0 38.46 59.190 6.00 4.00 6.810 176 ATACACAACACACATGTATATGCACA 121 26 0 34.62 59.190 6.00 4.00 6.810 177 TCCATACACAACACACATGTATATGC 124 26 0 38.46 60.811 6.00 4.00 6.811 178 CACAATACCACACATGTTTAACAG 99 24 0 37.50 57.140 7.00 5.00 6.860 179 ACATCCATACACAACACACATGTAT 127 25 0 36.00 58.124 6.00 6.00 6.876 180 ACACACACACACAACATACACTCT 222 24 0 41.67 57.096 2.00 1.00 6.904 181 GTATATGCACAATACCACACATGTTT 106 26 0 34.62 59.095 6.00 6.00 6.905 182 TCTACACACACACACAACATACACTC 225 26 0 42.31 59.077 2.00 0.00 6.923 183 AAGCTACATCCATACACAACACACAT 132 26 0 38.46 60.948 4.00 2.00 6.948 184 TATATGCACAATACCACACATGTT 105 24 0 33.33 57.002 6.00 4.00 6.998 185 TCTACACACACACACAACATACACTCT 225 27 0 40.74 60.001 2.00 1.00 7.001 186 ACACACACACACAACATACACTCTAGA 222 27 0 40.74 60.001 6.00 6.00 7.001 187 CACACACACACAACATACACTCTAGAC 221 27 0 44.44 60.001 6.00 4.00 7.001 188 ACACACACACAACATACACTCTAGACA 220 27 0 40.74 60.001 6.00 2.00 7.001 189 CACACACACAACATACACTCTAGACAC 219 27 0 44.44 60.001 6.00 0.00 7.001 190 ACACACACAACATACACTCTAGACACA 218 27 0 40.74 60.001 6.00 0.00 7.001 191 ACAACACACAGTCAAGAAGCTACATC 148 26 0 42.31 61.001 4.00 2.00 7.001 192 ATACACAACAGACACACACACACAAC 169 26 0 42.31 61.121 2.00 0.00 7.121 193 CATGTATATGCACAATACCACACATG 109 26 0 38.46 61.147 6.00 4.00 7.147 194 AACACACATGTATATGCACAATACC 115 25 0 36.00 57.851 6.00 4.00 7.149 195 GTATATGCACAATACCACACATGTT 106 25 0 36.00 57.851 6.00 4.00 7.149 196 CAAGAAGCTACATCCATACACAACAC 136 26 0 42.31 61.157 4.00 0.00 7.157 197 AAGAAGCTACATCCATACACAACACA 135 26 0 38.46 61.157 4.00 0.00 7.157 198 TACATCCATACACAACACACATGTA 128 25 0 36.00 57.841 6.00 4.00 7.159 199 CTACATCCATACACAACACACATGTA 129 26 0 38.46 58.815 6.00 4.00 7.185 200 ATATGCACAATACCACACATGTTTAAC 104 27 0 33.33 60.227 6.00 5.00 7.227 201 CATCCATACACAACACACATGTATATG 126 27 0 37.04 60.230 6.00 6.00 7.230 202 AACACACGTGTATATACACAACAGACA 182 27 0 37.04 59.748 12.00 1.00 7.252 203 TGCACAATACCACACATGTTTAACA 101 25 0 36.00 62.264 7.00 7.00 7.264 204 ACACACAGTCAAGAAGCTACATCCA 145 25 0 44.00 62.322 4.00 0.00 7.322 205 CACACGTGTATATACACAACAGACA 180 25 0 40.00 57.664 12.00 1.00 7.336 206 CACGTGTATATACACAACAGACACA 178 25 0 40.00 57.664 12.00 1.00 7.336 207 CGTGTATATACACAACAGACACACA 176 25 0 40.00 57.664 12.00 0.00 7.336 208 ATACACAACACACATGTATATGCACAA 121 27 0 33.33 60.389 6.00 2.00 7.389 209 TACACAACACACATGTATATGCACAAT 120 27 0 33.33 60.389 6.00 2.00 7.389 210 ACACAACACACATGTATATGCACAATA 119 27 0 33.33 60.389 6.00 4.00 7.389 211 CACAACACACATGTATATGCACAATAC 118 27 0 37.04 60.389 6.00 6.00 7.389 212 AGACACACACACACAACACACAGTC 160 25 0 48.00 62.414 3.00 1.00 7.414 213 TATATGCACAATACCACACATGTTTAA 105 27 0 29.63 59.560 6.00 4.00 7.440 214 ACACACAACACACAGTCAAGAAGCT 152 25 0 44.00 62.443 4.00 4.00 7.443 215 GATCTACACACACACACAACATACA 227 25 0 40.00 57.548 4.00 0.00 7.452 216 ACACACGTGTATATACACAACAGACA 181 26 0 38.46 58.508 12.00 1.00 7.492 217 CACACGTGTATATACACAACAGACAC 180 26 0 42.31 58.508 12.00 3.00 7.492 218 ACACGTGTATATACACAACAGACACA 179 26 0 38.46 58.508 12.00 1.00 7.492 219 CACGTGTATATACACAACAGACACAC 178 26 0 42.31 58.508 12.00 1.00 7.492 220 ACGTGTATATACACAACAGACACACA 177 26 0 38.46 58.508 12.00 0.00 7.492 221 CGTGTATATACACAACAGACACACAC 176 26 0 42.31 58.508 12.00 0.00 7.492 222 CACACACAACATACACTCTAGACACAT 217 27 0 40.74 59.502 6.00 2.00 7.498 223 ATGTATATGCACAATACCACACATGTT 108 27 0 33.33 60.562 6.00 4.00 7.562 224 GATCTACACACACACACAACATACAC 227 26 0 42.31 58.431 4.00 0.00 7.569 225 ATATACACAACAGACACACACACACAA 171 27 0 37.04 60.577 4.00 0.00 7.577 226 TATATGCACAATACCACACATGTTTA 105 26 0 30.77 58.395 6.00 4.00 7.605 227 GATCTACACACACACACAACATACACT 227 27 0 40.74 59.376 4.00 1.00 7.624 228 ATCTACACACACACACAACATACACTC 226 27 0 40.74 59.376 2.00 0.00 7.624 229 CACACACACACAACATACACTCTAG 221 25 0 44.00 57.370 4.00 4.00 7.630 230 TGTATATACACAACAGACACACACACA 174 27 0 37.04 59.363 10.00 0.00 7.637 231 TATATACACAACAGACACACACACACA 172 27 0 37.04 59.363 6.00 0.00 7.637 232 CATCCATACACAACACACATGTATA 126 25 0 36.00 57.352 6.00 4.00 7.648 233 TCCATACACAACACACATGTATATG 124 25 0 36.00 57.352 6.00 6.00 7.648 234 CCATACACAACACACATGTATATGCA 123 26 0 38.46 61.654 6.00 6.00 7.654 235 CACACATGTATATGCACAATACCACA 113 26 0 38.46 61.654 6.00 2.00 7.654 236 CACATGTATATGCACAATACCACACA 111 26 0 38.46 61.654 6.00 0.00 7.654 237 ACACACGTGTATATACACAACAGACAC 181 27 0 40.74 59.293 12.00 3.00 7.707 238 ACACGTGTATATACACAACAGACACAC 179 27 0 40.74 59.293 12.00 1.00 7.707 239 ACGTGTATATACACAACAGACACACAC 177 27 0 40.74 59.293 12.00 0.00 7.707 240 CACACAGTCAAGAAGCTACATCCATA 144 26 0 42.31 61.740 4.00 2.00 7.740 241 CACAGTCAAGAAGCTACATCCATACA 142 26 0 42.31 61.740 4.00 0.00 7.740 242 CAGTCAAGAAGCTACATCCATACACA 140 26 0 42.31 61.740 4.00 0.00 7.740 243 CTACACACACACACAACATACACTCT 224 26 0 42.31 58.251 2.00 1.00 7.749 244 ACACACACACACAACATACACTCTAG 222 26 0 42.31 58.251 4.00 4.00 7.749 245 TCTACACACACACACAACATACACT 225 25 0 40.00 57.217 2.00 1.00 7.783 246 CTACACACACACACAACATACACTC 224 25 0 44.00 57.217 2.00 0.00 7.783 247 TACACACACACACAACATACACTCT 223 25 0 40.00 57.217 2.00 1.00 7.783 248 ACACACACACACAACATACACTCTA 222 25 0 40.00 57.217 3.00 3.00 7.783 249 TACATCCATACACAACACACATGTAT 128 26 0 34.62 58.200 6.00 6.00 7.800 250 ACATCCATACACAACACACATGTATA 127 26 0 34.62 58.200 6.00 4.00 7.800 251 CACAACACACAGTCAAGAAGCTACAT 149 26 0 42.31 61.844 4.00 4.00 7.844 252 GAACACACGTGTATATACACAACAGA 183 26 0 38.46 58.139 12.00 4.00 7.861 253 GTATATGCACAATACCACACATGTTTA 106 27 0 33.33 59.133 6.00 4.00 7.867 254 CTACATCCATACACAACACACATGTAT 129 27 0 37.04 59.113 6.00 6.00 7.887 255 ACACACACACAACACACAGTCAAGA 156 25 0 44.00 62.889 3.00 2.00 7.889 256 ACAACACACATGTATATGCACAATA 117 25 0 32.00 57.073 6.00 4.00 7.927 257 CAACACACATGTATATGCACAATAC 116 25 0 36.00 57.073 6.00 6.00 7.927 258 AGAACACACGTGTATATACACAACAGA 184 27 0 37.04 59.049 12.00 4.00 7.951 259 ATCCATACACAACACACATGTATATGC 125 27 0 37.04 61.010 6.00 4.00 8.010 260 ACAACACACATGTATATGCACAATACC 117 27 0 37.04 61.034 6.00 4.00 8.034 261 AACACACATGTATATGCACAATACCAC 115 27 0 37.04 61.034 6.00 2.00 8.034 262 CACACGTGTATATACACAACAGACACA 180 27 0 40.74 61.042 12.00 1.00 8.042 263 CACGTGTATATACACAACAGACACACA 178 27 0 40.74 61.042 12.00 0.00 8.042 264 CGTGTATATACACAACAGACACACACA 176 27 0 40.74 61.042 12.00 0.00 8.042 265 TCAAGAAGCTACATCCATACACAACA 137 26 0 38.46 62.056 4.00 0.00 8.056 266 GAACACACGTGTATATACACAACAGAC 183 27 0 40.74 58.926 12.00 2.00 8.074 267 ACAACACACATGTATATGCACAATAC 117 26 0 34.62 57.913 6.00 6.00 8.087 268 TATACACAACAGACACACACACACAAC 170 27 0 40.74 61.092 4.00 0.00 8.092 269 AGTCAAGAAGCTACATCCATACACAAC 139 27 0 40.74 61.126 4.00 0.00 8.126 270 GCTACATCCATACACAACACACATGT 130 26 0 42.31 62.163 6.00 6.00 8.163 271 GCACAATACCACACATGTTTAACAGA 100 26 0 38.46 62.185 7.00 3.00 8.185 272 AGCTACATCCATACACAACACACATG 131 26 0 42.31 62.266 4.00 4.00 8.266 273 CATCCATACACAACACACATGTATAT 126 26 0 34.62 57.730 6.00 6.00 8.270 274 ATCCATACACAACACACATGTATATG 125 26 0 34.62 57.730 6.00 6.00 8.270 275 ACACACAACACACAGTCAAGAAGCTA 152 26 0 42.31 62.367 4.00 2.00 8.367 276 CACACAACACACAGTCAAGAAGCTAC 151 26 0 46.15 62.367 4.00 4.00 8.367 277 ACACAACACACAGTCAAGAAGCTACA 150 26 0 42.31 62.367 4.00 2.00 8.367 278 GAAGCTACATCCATACACAACACACA 133 26 0 42.31 62.368 4.00 0.00 8.368 279 ATCTACACACACACACAACATACACT 226 26 0 38.46 57.620 2.00 1.00 8.380 280 ATGCACAATACCACACATGTTTAACA 102 26 0 34.62 62.397 7.00 7.00 8.397 281 TGTATATGCACAATACCACACATGTTT 107 27 0 33.33 61.445 6.00 6.00 8.445 282 ACACACAGTCAAGAAGCTACATCCAT 145 26 0 42.31 62.454 4.00 2.00 8.454 283 ACATCCATACACAACACACATGTATAT 127 27 0 33.33 58.526 6.00 6.00 8.474 284 AGAACACACGTGTATATACACAACAG 184 26 0 38.46 57.375 12.00 6.00 8.625 285 ACACACACACAACATACACTCTAGAC 220 26 0 42.31 57.364 6.00 4.00 8.636 286 ACACACACAACATACACTCTAGACAC 218 26 0 42.31 57.364 6.00 0.00 8.636 287 TACACACACACACAACATACACTCTA 223 26 0 38.46 57.330 3.00 3.00 8.670 288 CTACACACACACACAACATACACTCTA 224 27 0 40.74 58.323 3.00 3.00 8.677 289 TACACACACACACAACATACACTCTAG 223 27 0 40.74 58.323 4.00 4.00 8.677 290 CATACACAACACACATGTATATGCACA 122 27 0 37.04 61.688 6.00 4.00 8.688 291 TACATCCATACACAACACACATGTATA 128 27 0 33.33 58.271 6.00 4.00 8.729 292 AACACACGTGTATATACACAACAGAC 182 26 0 38.46 57.237 12.00 2.00 8.763 293 AAGAAGCTACATCCATACACAACACAC 135 27 0 40.74 61.811 4.00 0.00 8.811 294 CACATGTATATGCACAATACCACACAT 111 27 0 37.04 61.814 6.00 2.00 8.814 295 ACATGTATATGCACAATACCACACATG 110 27 0 37.04 61.814 6.00 4.00 8.814 296 CATGTATATGCACAATACCACACATGT 109 27 0 37.04 61.814 6.00 6.00 8.814 297 CACAATACCACACATGTTTAACAGACA 99 27 0 37.04 62.089 7.00 3.00 9.089 298 GCTACATCCATACACAACACACATGTA 130 27 0 40.74 62.100 6.00 4.00 9.100 299 CCATACACAACACACATGTATATGCAC 123 27 0 40.74 62.312 6.00 4.00 9.312 300 ACACACATGTATATGCACAATACCACA 114 27 0 37.04 62.312 6.00 2.00 9.312 301 CACACATGTATATGCACAATACCACAC 113 27 0 40.74 62.312 6.00 0.00 9.312 302 ACACATGTATATGCACAATACCACACA 112 27 0 37.04 62.312 6.00 0.00 9.312 303 TATGCACAATACCACACATGTTTAACA 103 27 0 33.33 62.329 7.00 7.00 9.329 304 ACACACAGTCAAGAAGCTACATCCATA 145 27 0 40.74 62.383 4.00 2.00 9.383 305 CACACAGTCAAGAAGCTACATCCATAC 144 27 0 44.44 62.383 4.00 0.00 9.383 306 ACACAGTCAAGAAGCTACATCCATACA 143 27 0 40.74 62.383 4.00 0.00 9.383 307 CACAGTCAAGAAGCTACATCCATACAC 142 27 0 44.44 62.383 4.00 0.00 9.383 308 ACAGTCAAGAAGCTACATCCATACACA 141 27 0 40.74 62.383 4.00 0.00 9.383 309 GTGTATATACACAACAGACACACACAC 175 27 0 40.74 57.575 12.00 0.00 9.425 310 GTATATACACAACAGACACACACACAC 173 27 0 40.74 57.575 8.00 0.00 9.425 311 GAAGCTACATCCATACACAACACACAT 133 27 0 40.74 62.494 4.00 2.00 9.494 312 ACACAACACACAGTCAAGAAGCTACAT 150 27 0 40.74 62.495 4.00 4.00 9.495 313 TAGAACACACGTGTATATACACAACAG 185 27 0 37.04 57.472 12.00 6.00 9.528 314 GTCAAGAAGCTACATCCATACACAACA 138 27 0 40.74 62.685 4.00 0.00 9.685 315 TCAAGAAGCTACATCCATACACAACAC 137 27 0 40.74 62.685 4.00 0.00 9.685 316 CAACACACATGTATATGCACAATACCA 116 27 0 37.04 62.716 6.00 4.00 9.716 317 CAGTCAAGAAGCTACATCCATACACAA 140 27 0 40.74 62.778 4.00 0.00 9.778 318 GCACAATACCACACATGTTTAACAGAC 100 27 0 40.74 62.806 7.00 1.00 9.806 319 AGCTACATCCATACACAACACACATGT 131 27 0 40.74 62.902 6.00 6.00 9.902 primer3-2.3.6/test/primer_list_last/SP01-06-with-repeat3.for0000664000077300007730000001025012231156244022336 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 2.909 1 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 3.033 2 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 3.626 3 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 4.023 4 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 4.199 5 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 4.207 6 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 4.458 7 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 4.533 8 GAATCGCAATCAAATTTTTTC 33 21 0 28.57 56.457 6.00 2.00 4.543 9 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 4.596 10 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 4.923 11 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 5.202 12 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 5.202 13 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 5.202 14 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 5.202 15 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 5.211 16 AATCGCAATCAAATTTTTTCT 34 21 0 23.81 55.693 6.00 2.00 5.307 17 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 5.323 18 AATCGCAATCAAATTTTTTC 34 20 0 25.00 54.571 6.00 2.00 5.429 19 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 5.439 20 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 5.502 21 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 5.544 22 ATCGCAATCAAATTTTTTCT 35 20 0 25.00 54.168 6.00 2.00 5.832 23 AATCGCAATCAAATTTTTTCTA 34 22 0 22.73 55.850 6.00 2.00 6.150 24 TCGCAATCAAATTTTTTCTA 36 20 0 25.00 53.814 6.00 2.00 6.186 25 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 6.204 26 TCGCAATCAAATTTTTTCTAT 36 21 0 23.81 54.389 6.00 2.00 6.611 27 ATCGCAATCAAATTTTTTCTA 35 21 0 23.81 54.389 6.00 2.00 6.611 28 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 6.694 29 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 6.843 30 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 7.226 31 TCGCAATCAAATTTTTTCT 36 19 0 26.32 53.559 6.00 2.00 7.441 32 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 7.463 33 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 7.653 34 ATCGCAATCAAATTTTTTC 35 19 0 26.32 52.911 6.00 2.00 8.089 35 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 8.199 36 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 8.382 37 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 8.397 38 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 8.574 39 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 8.652 40 TCGCAATCAAATTTTTTCTATGACCT 36 26 0 30.77 63.123 6.00 1.00 9.123 41 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 9.235 42 AATTATGAATCGCAATCAA 27 19 0 26.32 51.609 5.00 2.00 9.391 43 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 9.507 44 TCGCAATCAAATTTTTTC 36 18 0 27.78 52.197 6.00 2.00 9.803 45 ATCGCAATCAAATTTTTTCTATGACCT 35 27 0 29.63 63.204 6.00 1.00 10.204 46 AATCGCAATCAAATTTTTTCTATGACC 34 27 0 29.63 63.467 6.00 0.00 10.467 47 TGAATCGCAATCAAATTTTTTCTATGA 32 27 0 25.93 63.763 6.00 2.00 10.763 48 TCGCAATCAAATTTTTTCTATGACCTG 36 27 0 33.33 65.188 6.00 1.00 12.188 49 TATGAATCGCAATCAAAT 30 18 0 27.78 49.586 4.00 2.00 12.414 50 ATTATGAATCGCAATCAA 28 18 0 27.78 49.586 5.00 2.00 12.414 primer3-2.3.6/test/primer_list_last/SP01-19.txt.rev0000664000077300007730000001063112231156244020657 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TCCCTCATCCAGTGCTCTTT 184 20 0 50.00 59.803 4.00 0.00 0.197 1 CCCTCATCCAGTGCTCTTTC 183 20 0 55.00 59.803 4.00 0.00 0.197 2 CAGTGCTCTTTCTGCCAGTG 175 20 0 55.00 59.770 5.00 3.00 0.230 3 CCAGTGCTCTTTCTGCCAGT 176 20 0 55.00 60.589 5.00 3.00 0.589 4 TGCTCTTTCTGCCAGTGTCA 172 20 0 50.00 60.745 5.00 5.00 0.745 5 GATCCCTCATCCAGTGCTCT 186 20 0 55.00 59.210 4.00 4.00 0.790 6 TCATCCAGTGCTCTTTCTGC 180 20 0 50.00 59.116 4.00 2.00 0.884 7 CATCCAGTGCTCTTTCTGCC 179 20 0 55.00 60.945 4.00 1.00 0.945 8 ATCCAGTGCTCTTTCTGCCA 178 20 0 50.00 60.945 4.00 3.00 0.945 9 CCTCATCCAGTGCTCTTTCTG 182 21 0 52.38 59.997 4.00 2.00 1.003 10 ATCCCTCATCCAGTGCTCTTT 185 21 0 47.62 60.088 4.00 0.00 1.088 11 CTCATCCAGTGCTCTTTCTGC 181 21 0 52.38 60.149 4.00 2.00 1.149 12 CCAGTGCTCTTTCTGCCAG 176 19 0 57.89 59.710 5.00 5.00 1.290 13 ATCCCTCATCCAGTGCTCTT 185 20 0 50.00 58.693 4.00 2.00 1.307 14 AGTGCTCTTTCTGCCAGTGTC 174 21 0 52.38 59.661 5.00 3.00 1.339 15 GTGCTCTTTCTGCCAGTGTC 173 20 0 55.00 58.596 5.00 3.00 1.404 16 GCTCTTTCTGCCAGTGTCAC 171 20 0 55.00 58.596 5.00 5.00 1.404 17 GATCCCTCATCCAGTGCTCTT 186 21 0 52.38 60.611 4.00 2.00 1.611 18 CAGTGCTCTTTCTGCCAGTGT 175 21 0 52.38 60.638 5.00 1.00 1.638 19 TCCAGTGCTCTTTCTGCCAG 177 20 0 55.00 61.674 5.00 5.00 1.674 20 TCCAGTGCTCTTTCTGCCA 177 19 0 52.63 60.694 4.00 3.00 1.694 21 CCCTCATCCAGTGCTCTTTCT 183 21 0 52.38 60.771 4.00 0.00 1.771 22 GGCCCTCTGGTTAACCGT 148 18 0 61.11 59.927 8.00 4.00 2.073 23 AGTGCTCTTTCTGCCAGTGT 174 20 0 50.00 57.670 5.00 1.00 2.330 24 GTGCTCTTTCTGCCAGTGTCA 173 21 0 52.38 61.588 5.00 5.00 2.588 25 TGCTCTTTCTGCCAGTGTCAC 172 21 0 52.38 61.588 5.00 5.00 2.588 26 TCCCTCATCCAGTGCTCTTTC 184 21 0 52.38 61.686 4.00 0.00 2.686 27 TCCCTCATCCAGTGCTCTT 184 19 0 52.63 58.306 4.00 2.00 2.694 28 ATCCAGTGCTCTTTCTGCCAG 178 21 0 52.38 61.860 5.00 5.00 2.860 29 GCCCTCTGGTTAACCGTG 147 18 0 61.11 59.086 8.00 6.00 2.914 30 GATCCCTCATCCAGTGCTC 186 19 0 57.89 58.080 4.00 2.00 2.920 31 CCTCATCCAGTGCTCTTTCT 182 20 0 50.00 57.032 4.00 0.00 2.968 32 ATCCAGTGCTCTTTCTGCC 178 19 0 52.63 57.954 4.00 1.00 3.046 33 CCCTCATCCAGTGCTCTTT 183 19 0 52.63 57.795 4.00 0.00 3.205 34 TGCTCTTTCTGCCAGTGTC 172 19 0 52.63 57.590 5.00 3.00 3.410 35 GCTCTTTCTGCCAGTGTCA 171 19 0 52.63 57.590 5.00 5.00 3.410 36 CCAGTGCTCTTTCTGCCA 176 18 0 55.56 58.588 4.00 3.00 3.412 37 TCCAGTGCTCTTTCTGCCAGT 177 21 0 52.38 62.447 5.00 3.00 3.447 38 TTGGCCCTCTGGTTAACC 150 18 0 55.56 58.487 8.00 8.00 3.513 39 TGGCCCTCTGGTTAACCGT 149 19 0 57.89 62.761 8.00 4.00 3.761 40 GGCCCTCTGGTTAACCGTG 148 19 0 63.16 62.761 8.00 6.00 3.761 41 TCATCCAGTGCTCTTTCTGCC 180 21 0 52.38 62.794 4.00 1.00 3.794 42 GATCCCTCATCCAGTGCTCTTT 186 22 0 50.00 61.864 4.00 0.00 3.864 43 ATCCCTCATCCAGTGCTCTTTC 185 22 0 50.00 61.864 4.00 0.00 3.864 44 ATCCCTCATCCAGTGCTCT 185 19 0 52.63 57.128 4.00 4.00 3.872 45 CATCCAGTGCTCTTTCTGC 179 19 0 52.63 57.014 4.00 2.00 3.986 46 TGGCCCTCTGGTTAACCG 149 18 0 61.11 62.001 8.00 6.00 4.001 47 GTGCTCTTTCTGCCAGTGTCAC 173 22 0 54.55 62.364 5.00 5.00 4.364 48 CAGTGCTCTTTCTGCCAGTGTC 175 22 0 54.55 62.479 5.00 3.00 4.479 49 AGTGCTCTTTCTGCCAGTGTCA 174 22 0 50.00 62.479 5.00 5.00 4.479 50 TCCAGTGCTCTTTCTGCC 177 18 0 55.56 57.507 4.00 1.00 4.493 51 TCCCTCATCCAGTGCTCTTTCT 184 22 0 50.00 62.539 4.00 0.00 4.539 52 ATCCAGTGCTCTTTCTGCCAGT 178 22 0 50.00 62.585 5.00 3.00 4.585 53 ATCCCTCATCCAGTGCTCTTTCT 185 23 0 47.83 62.667 4.00 0.00 5.667 primer3-2.3.6/test/primer_list_last/included_test_w_middle.int0000664000077300007730000007763312231156244023636 0ustar ioanaioanaACCEPTABLE INTERNAL OLIGOS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGTATGTTGTGTGTGTGTGTAGATC 223 25 0 40.00 57.548 4.00 4.00 7.452 1 GTGTATGTTGTGTGTGTGTGTAGATC 222 26 0 42.31 58.431 4.00 4.00 7.569 2 AGTGTATGTTGTGTGTGTGTGTAGATC 221 27 0 40.74 59.376 4.00 4.00 7.624 3 AGTGTATGTTGTGTGTGTGTGTAGAT 221 26 0 38.46 57.620 2.00 2.00 8.380 4 GAGTGTATGTTGTGTGTGTGTGTAGAT 220 27 0 40.74 59.376 2.00 2.00 7.624 5 AGTGTATGTTGTGTGTGTGTGTAGA 221 25 0 40.00 57.217 2.00 2.00 7.783 6 GAGTGTATGTTGTGTGTGTGTGTAGA 220 26 0 42.31 59.077 2.00 2.00 6.923 7 AGAGTGTATGTTGTGTGTGTGTGTAGA 219 27 0 40.74 60.001 2.00 2.00 7.001 8 GAGTGTATGTTGTGTGTGTGTGTAG 220 25 0 44.00 57.217 2.00 0.00 7.783 9 AGAGTGTATGTTGTGTGTGTGTGTAG 219 26 0 42.31 58.251 2.00 0.00 7.749 10 TAGAGTGTATGTTGTGTGTGTGTGTAG 218 27 0 40.74 58.323 3.00 0.00 8.677 11 AGAGTGTATGTTGTGTGTGTGTGTA 219 25 0 40.00 57.217 2.00 2.00 7.783 12 TAGAGTGTATGTTGTGTGTGTGTGTA 218 26 0 38.46 57.330 3.00 2.00 8.670 13 CTAGAGTGTATGTTGTGTGTGTGTGTA 217 27 0 40.74 58.323 4.00 2.00 8.677 14 AGAGTGTATGTTGTGTGTGTGTGT 219 24 0 41.67 57.096 2.00 0.00 6.904 15 TAGAGTGTATGTTGTGTGTGTGTGT 218 25 0 40.00 57.217 3.00 0.00 7.783 16 CTAGAGTGTATGTTGTGTGTGTGTGT 217 26 0 42.31 58.251 4.00 0.00 7.749 17 TCTAGAGTGTATGTTGTGTGTGTGTGT 216 27 0 40.74 60.001 6.00 0.00 7.001 18 CTAGAGTGTATGTTGTGTGTGTGTG 217 25 0 44.00 57.370 4.00 0.00 7.630 19 TCTAGAGTGTATGTTGTGTGTGTGTG 216 26 0 42.31 59.211 6.00 0.00 6.789 20 GTCTAGAGTGTATGTTGTGTGTGTGTG 215 27 0 44.44 60.001 6.00 0.00 7.001 21 GTCTAGAGTGTATGTTGTGTGTGTGT 215 26 0 42.31 57.364 6.00 0.00 8.636 22 TGTCTAGAGTGTATGTTGTGTGTGTGT 214 27 0 40.74 60.001 6.00 0.00 7.001 23 TGTCTAGAGTGTATGTTGTGTGTGTG 214 26 0 42.31 59.211 6.00 0.00 6.789 24 GTGTCTAGAGTGTATGTTGTGTGTGTG 213 27 0 44.44 60.001 6.00 0.00 7.001 25 GTGTCTAGAGTGTATGTTGTGTGTGT 213 26 0 42.31 57.364 6.00 0.00 8.636 26 TGTGTCTAGAGTGTATGTTGTGTGTGT 212 27 0 40.74 60.001 6.00 0.00 7.001 27 TGTGTCTAGAGTGTATGTTGTGTGTG 212 26 0 42.31 59.211 6.00 0.00 6.789 28 CTGTGTCTAGAGTGTATGTTGTGTGTG 211 27 0 44.44 60.122 6.00 0.00 7.122 29 CTGTGTCTAGAGTGTATGTTGTGTGT 211 26 0 42.31 57.508 6.00 0.00 8.492 30 TGTGTATATACACGTGTGTTCTATGTC 183 27 0 37.04 57.285 12.00 2.00 9.715 31 CTGTTGTGTATATACACGTGTGTTCTA 179 27 0 37.04 57.472 12.00 6.00 9.528 32 CTGTTGTGTATATACACGTGTGTTCT 179 26 0 38.46 57.375 12.00 4.00 8.625 33 TCTGTTGTGTATATACACGTGTGTTCT 178 27 0 37.04 59.049 12.00 4.00 7.951 34 TCTGTTGTGTATATACACGTGTGTTC 178 26 0 38.46 58.139 12.00 4.00 7.861 35 GTCTGTTGTGTATATACACGTGTGTTC 177 27 0 40.74 58.926 12.00 4.00 8.074 36 GTCTGTTGTGTATATACACGTGTGTT 177 26 0 38.46 57.237 12.00 6.00 8.763 37 TGTCTGTTGTGTATATACACGTGTGTT 176 27 0 37.04 59.748 12.00 6.00 7.252 38 TGTCTGTTGTGTATATACACGTGTGT 176 26 0 38.46 58.508 12.00 8.00 7.492 39 GTGTCTGTTGTGTATATACACGTGTGT 175 27 0 40.74 59.293 12.00 8.00 7.707 40 TGTCTGTTGTGTATATACACGTGTG 176 25 0 40.00 57.664 12.00 6.00 7.336 41 GTGTCTGTTGTGTATATACACGTGTG 175 26 0 42.31 58.508 12.00 6.00 7.492 42 TGTGTCTGTTGTGTATATACACGTGTG 174 27 0 40.74 61.042 12.00 6.00 8.042 43 TGTGTCTGTTGTGTATATACACGTGT 174 26 0 38.46 58.508 12.00 8.00 7.492 44 GTGTGTCTGTTGTGTATATACACGTGT 173 27 0 40.74 59.293 12.00 8.00 7.707 45 TGTGTCTGTTGTGTATATACACGTG 174 25 0 40.00 57.664 12.00 6.00 7.336 46 GTGTGTCTGTTGTGTATATACACGTG 173 26 0 42.31 58.508 12.00 6.00 7.492 47 TGTGTGTCTGTTGTGTATATACACGTG 172 27 0 40.74 61.042 12.00 6.00 8.042 48 TGTGTGTCTGTTGTGTATATACACGT 172 26 0 38.46 58.508 12.00 8.00 7.492 49 GTGTGTGTCTGTTGTGTATATACACGT 171 27 0 40.74 59.293 12.00 8.00 7.707 50 GTGTGTGTGTGTCTGTTGTGTATATAC 167 27 0 40.74 57.575 8.00 8.00 9.425 51 TGTGTGTGTGTGTCTGTTGTGTATATA 166 27 0 37.04 59.363 6.00 6.00 7.637 52 TGTGTGTGTGTGTCTGTTGTGTATAT 166 26 0 38.46 59.329 4.00 4.00 6.671 53 TTGTGTGTGTGTGTCTGTTGTGTATAT 165 27 0 37.04 60.577 4.00 4.00 7.577 54 TGTGTGTGTGTGTCTGTTGTGTATA 166 25 0 40.00 59.013 4.00 4.00 5.987 55 TTGTGTGTGTGTGTCTGTTGTGTATA 165 26 0 38.46 60.342 4.00 4.00 6.342 56 GTTGTGTGTGTGTGTCTGTTGTGTATA 164 27 0 40.74 61.092 4.00 4.00 8.092 57 TGTGTGTGTGTGTCTGTTGTGTAT 166 24 0 41.67 58.961 2.00 2.00 5.039 58 TTGTGTGTGTGTGTCTGTTGTGTAT 165 25 0 40.00 60.344 2.00 2.00 5.344 59 GTTGTGTGTGTGTGTCTGTTGTGTAT 164 26 0 42.31 61.121 2.00 2.00 7.121 60 TGTGTGTGTGTGTCTGTTGTGTA 166 23 0 43.48 58.590 2.00 2.00 4.410 61 TTGTGTGTGTGTGTCTGTTGTGTA 165 24 0 41.67 60.073 2.00 2.00 4.073 62 GTTGTGTGTGTGTGTCTGTTGTGTA 164 25 0 44.00 60.904 2.00 2.00 5.904 63 TGTGTGTGTGTGTCTGTTGTGT 166 22 0 45.45 58.514 1.00 0.00 3.486 64 TTGTGTGTGTGTGTCTGTTGTGT 165 23 0 43.48 60.064 1.00 0.00 3.064 65 GTTGTGTGTGTGTGTCTGTTGTGT 164 24 0 45.83 60.929 1.00 0.00 4.929 66 TGTGTGTGTGTGTCTGTTGTG 166 21 0 47.62 57.434 1.00 0.00 3.566 67 TTGTGTGTGTGTGTCTGTTGTG 165 22 0 45.45 59.121 1.00 0.00 2.879 68 GTTGTGTGTGTGTGTCTGTTGTG 164 23 0 47.83 60.064 1.00 0.00 3.064 69 TGTTGTGTGTGTGTGTCTGTTGT 163 23 0 43.48 60.064 1.00 0.00 3.064 70 GTGTTGTGTGTGTGTGTCTGTTGT 162 24 0 45.83 60.929 1.00 0.00 4.929 71 TGTTGTGTGTGTGTGTCTGTTG 163 22 0 45.45 59.121 1.00 0.00 2.879 72 GTGTTGTGTGTGTGTGTCTGTTG 162 23 0 47.83 60.064 1.00 0.00 3.064 73 TGTGTTGTGTGTGTGTGTCTGTT 161 23 0 43.48 60.064 1.00 0.00 3.064 74 GTGTGTTGTGTGTGTGTGTCTGTT 160 24 0 45.83 60.929 1.00 0.00 4.929 75 TGTGTTGTGTGTGTGTGTCTGT 161 22 0 45.45 58.514 1.00 0.00 3.486 76 GTGTGTTGTGTGTGTGTGTCTGT 160 23 0 47.83 59.499 1.00 0.00 3.501 77 TGTGTGTTGTGTGTGTGTGTCTGT 159 24 0 45.83 62.520 1.00 0.00 6.520 78 TGTGTTGTGTGTGTGTGTCTG 161 21 0 47.62 57.434 1.00 1.00 3.566 79 GTGTGTTGTGTGTGTGTGTCTG 160 22 0 50.00 58.514 1.00 1.00 3.486 80 TGTGTGTTGTGTGTGTGTGTCTG 159 23 0 47.83 61.701 1.00 1.00 4.701 81 CTGTGTGTTGTGTGTGTGTGTCTG 158 24 0 50.00 62.641 1.00 1.00 6.641 82 TGTGTGTTGTGTGTGTGTGTCT 159 22 0 45.45 58.514 1.00 0.00 3.486 83 CTGTGTGTTGTGTGTGTGTGTCT 158 23 0 47.83 59.650 1.00 0.00 3.350 84 ACTGTGTGTTGTGTGTGTGTGTCT 157 24 0 45.83 60.539 3.00 0.00 4.539 85 GACTGTGTGTTGTGTGTGTGTGTCT 156 25 0 48.00 62.414 3.00 0.00 7.414 86 TGTGTGTTGTGTGTGTGTGTC 159 21 0 47.62 57.244 1.00 1.00 3.756 87 CTGTGTGTTGTGTGTGTGTGTC 158 22 0 50.00 58.514 1.00 1.00 3.486 88 ACTGTGTGTTGTGTGTGTGTGTC 157 23 0 47.83 59.499 3.00 1.00 3.501 89 GACTGTGTGTTGTGTGTGTGTGTC 156 24 0 50.00 61.504 3.00 1.00 5.504 90 ACTGTGTGTTGTGTGTGTGTGT 157 22 0 45.45 57.352 3.00 0.00 4.648 91 GACTGTGTGTTGTGTGTGTGTGT 156 23 0 47.83 59.499 3.00 0.00 3.501 92 TGACTGTGTGTTGTGTGTGTGTGT 155 24 0 45.83 62.520 3.00 0.00 6.520 93 GACTGTGTGTTGTGTGTGTGTG 156 22 0 50.00 58.514 3.00 0.00 3.486 94 TGACTGTGTGTTGTGTGTGTGTG 155 23 0 47.83 61.701 3.00 0.00 4.701 95 TGACTGTGTGTTGTGTGTGTGT 155 22 0 45.45 58.514 3.00 0.00 3.486 96 TTGACTGTGTGTTGTGTGTGTGT 154 23 0 43.48 60.064 3.00 0.00 3.064 97 CTTGACTGTGTGTTGTGTGTGTGT 153 24 0 45.83 61.059 3.00 0.00 5.059 98 TCTTGACTGTGTGTTGTGTGTGTGT 152 25 0 44.00 62.889 3.00 0.00 7.889 99 TGACTGTGTGTTGTGTGTGTG 155 21 0 47.62 57.434 3.00 0.00 3.566 100 TTGACTGTGTGTTGTGTGTGTG 154 22 0 45.45 59.121 3.00 0.00 2.879 101 CTTGACTGTGTGTTGTGTGTGTG 153 23 0 47.83 60.208 3.00 0.00 3.208 102 TCTTGACTGTGTGTTGTGTGTGTG 152 24 0 45.83 62.141 3.00 0.00 6.141 103 CTTGACTGTGTGTTGTGTGTGT 153 22 0 45.45 57.090 3.00 0.00 4.910 104 TCTTGACTGTGTGTTGTGTGTGT 152 23 0 43.48 59.188 3.00 0.00 3.812 105 TTCTTGACTGTGTGTTGTGTGTGT 151 24 0 41.67 60.599 3.00 0.00 4.599 106 CTTCTTGACTGTGTGTTGTGTGTGT 150 25 0 44.00 61.508 3.00 0.00 6.508 107 TCTTGACTGTGTGTTGTGTGTG 152 22 0 45.45 58.217 3.00 0.00 3.783 108 TTCTTGACTGTGTGTTGTGTGTG 151 23 0 43.48 59.744 3.00 0.00 3.256 109 CTTCTTGACTGTGTGTTGTGTGTG 150 24 0 45.83 60.730 3.00 0.00 4.730 110 CTTCTTGACTGTGTGTTGTGTGT 150 23 0 43.48 57.837 3.00 0.00 5.163 111 GCTTCTTGACTGTGTGTTGTGTGT 149 24 0 45.83 61.613 3.00 0.00 5.613 112 AGCTTCTTGACTGTGTGTTGTGTGT 148 25 0 44.00 62.443 4.00 0.00 7.443 113 TAGCTTCTTGACTGTGTGTTGTGTGT 147 26 0 42.31 62.367 4.00 0.00 8.367 114 GCTTCTTGACTGTGTGTTGTGTG 149 23 0 47.83 60.832 3.00 0.00 3.832 115 AGCTTCTTGACTGTGTGTTGTGTG 148 24 0 45.83 61.730 4.00 0.00 5.730 116 TAGCTTCTTGACTGTGTGTTGTGTG 147 25 0 44.00 61.678 4.00 0.00 6.678 117 GTAGCTTCTTGACTGTGTGTTGTGTG 146 26 0 46.15 62.367 4.00 0.00 8.367 118 GCTTCTTGACTGTGTGTTGTGT 149 22 0 45.45 57.918 3.00 0.00 4.082 119 AGCTTCTTGACTGTGTGTTGTGT 148 23 0 43.48 58.981 4.00 0.00 4.019 120 TAGCTTCTTGACTGTGTGTTGTGT 147 24 0 41.67 59.030 4.00 0.00 4.970 121 GTAGCTTCTTGACTGTGTGTTGTGT 146 25 0 44.00 59.835 4.00 0.00 5.165 122 TGTAGCTTCTTGACTGTGTGTTGTGT 145 26 0 42.31 62.367 4.00 0.00 8.367 123 ATGTAGCTTCTTGACTGTGTGTTGTGT 144 27 0 40.74 62.495 4.00 0.00 9.495 124 AGCTTCTTGACTGTGTGTTGTG 148 22 0 45.45 58.075 4.00 0.00 3.925 125 TAGCTTCTTGACTGTGTGTTGTG 147 23 0 43.48 58.160 4.00 0.00 4.840 126 GTAGCTTCTTGACTGTGTGTTGTG 146 24 0 45.83 59.030 4.00 0.00 4.970 127 TGTAGCTTCTTGACTGTGTGTTGTG 145 25 0 44.00 61.678 4.00 0.00 6.678 128 ATGTAGCTTCTTGACTGTGTGTTGTG 144 26 0 42.31 61.844 4.00 0.00 7.844 129 TGTAGCTTCTTGACTGTGTGTTGT 145 24 0 41.67 59.030 4.00 0.00 4.970 130 ATGTAGCTTCTTGACTGTGTGTTGT 144 25 0 40.00 59.333 4.00 0.00 5.667 131 GATGTAGCTTCTTGACTGTGTGTTGT 143 26 0 42.31 61.001 4.00 0.00 7.001 132 TGTAGCTTCTTGACTGTGTGTTG 145 23 0 43.48 58.160 4.00 0.00 4.840 133 ATGTAGCTTCTTGACTGTGTGTTG 144 24 0 41.67 58.520 4.00 0.00 5.480 134 GATGTAGCTTCTTGACTGTGTGTTG 143 25 0 44.00 60.272 4.00 0.00 5.272 135 GATGTAGCTTCTTGACTGTGTGTT 143 24 0 41.67 57.626 4.00 0.00 6.374 136 GGATGTAGCTTCTTGACTGTGTGTT 142 25 0 44.00 60.962 4.00 0.00 5.962 137 GGATGTAGCTTCTTGACTGTGTGT 142 24 0 45.83 59.748 4.00 0.00 4.252 138 TGGATGTAGCTTCTTGACTGTGTGT 141 25 0 44.00 62.322 4.00 0.00 7.322 139 ATGGATGTAGCTTCTTGACTGTGTGT 140 26 0 42.31 62.454 4.00 0.00 8.454 140 TATGGATGTAGCTTCTTGACTGTGTGT 139 27 0 40.74 62.383 4.00 0.00 9.383 141 GGATGTAGCTTCTTGACTGTGTG 142 23 0 47.83 58.925 4.00 0.00 4.075 142 TGGATGTAGCTTCTTGACTGTGTG 141 24 0 45.83 61.619 4.00 0.00 5.619 143 ATGGATGTAGCTTCTTGACTGTGTG 140 25 0 44.00 61.791 4.00 0.00 6.791 144 TATGGATGTAGCTTCTTGACTGTGTG 139 26 0 42.31 61.740 4.00 0.00 7.740 145 GTATGGATGTAGCTTCTTGACTGTGTG 138 27 0 44.44 62.383 4.00 0.00 9.383 146 TGGATGTAGCTTCTTGACTGTGT 141 23 0 43.48 58.925 4.00 1.00 4.075 147 ATGGATGTAGCTTCTTGACTGTGT 140 24 0 41.67 59.238 4.00 1.00 4.762 148 TATGGATGTAGCTTCTTGACTGTGT 139 25 0 40.00 59.274 4.00 1.00 5.726 149 GTATGGATGTAGCTTCTTGACTGTGT 138 26 0 42.31 60.018 4.00 1.00 6.018 150 TGTATGGATGTAGCTTCTTGACTGTGT 137 27 0 40.74 62.383 4.00 1.00 9.383 151 TGGATGTAGCTTCTTGACTGTG 141 22 0 45.45 58.036 4.00 1.00 3.964 152 ATGGATGTAGCTTCTTGACTGTG 140 23 0 43.48 58.408 4.00 1.00 4.592 153 TATGGATGTAGCTTCTTGACTGTG 139 24 0 41.67 58.476 4.00 1.00 5.524 154 GTATGGATGTAGCTTCTTGACTGTG 138 25 0 44.00 59.274 4.00 1.00 5.726 155 TGTATGGATGTAGCTTCTTGACTGTG 137 26 0 42.31 61.740 4.00 1.00 7.740 156 GTGTATGGATGTAGCTTCTTGACTGTG 136 27 0 44.44 62.383 4.00 1.00 9.383 157 TGTATGGATGTAGCTTCTTGACTGT 137 25 0 40.00 59.274 4.00 3.00 5.726 158 GTGTATGGATGTAGCTTCTTGACTGT 136 26 0 42.31 60.018 4.00 3.00 6.018 159 TGTGTATGGATGTAGCTTCTTGACTGT 135 27 0 40.74 62.383 4.00 3.00 9.383 160 TGTATGGATGTAGCTTCTTGACTG 137 24 0 41.67 58.476 4.00 1.00 5.524 161 GTGTATGGATGTAGCTTCTTGACTG 136 25 0 44.00 59.274 4.00 1.00 5.726 162 TGTGTATGGATGTAGCTTCTTGACTG 135 26 0 42.31 61.740 4.00 1.00 7.740 163 TTGTGTATGGATGTAGCTTCTTGACTG 134 27 0 40.74 62.778 4.00 1.00 9.778 164 TGTGTATGGATGTAGCTTCTTGACT 135 25 0 40.00 59.274 4.00 1.00 5.726 165 TTGTGTATGGATGTAGCTTCTTGACT 134 26 0 38.46 60.455 4.00 1.00 6.455 166 GTTGTGTATGGATGTAGCTTCTTGACT 133 27 0 40.74 61.126 4.00 1.00 8.126 167 TGTGTATGGATGTAGCTTCTTGAC 135 24 0 41.67 58.339 4.00 1.00 5.661 168 TTGTGTATGGATGTAGCTTCTTGAC 134 25 0 40.00 59.615 4.00 1.00 5.385 169 GTTGTGTATGGATGTAGCTTCTTGAC 133 26 0 42.31 60.343 4.00 1.00 6.343 170 TGTTGTGTATGGATGTAGCTTCTTGAC 132 27 0 40.74 62.685 4.00 1.00 9.685 171 TGTGTATGGATGTAGCTTCTTGA 135 23 0 39.13 57.467 4.00 2.00 5.533 172 TTGTGTATGGATGTAGCTTCTTGA 134 24 0 37.50 58.834 4.00 2.00 5.166 173 GTTGTGTATGGATGTAGCTTCTTGA 133 25 0 40.00 59.615 4.00 2.00 5.385 174 TGTTGTGTATGGATGTAGCTTCTTGA 132 26 0 38.46 62.056 4.00 2.00 8.056 175 GTGTTGTGTATGGATGTAGCTTCTTGA 131 27 0 40.74 62.685 4.00 2.00 9.685 176 TTGTGTATGGATGTAGCTTCTTG 134 23 0 39.13 57.036 4.00 2.00 5.964 177 GTTGTGTATGGATGTAGCTTCTTG 133 24 0 41.67 57.904 4.00 2.00 6.096 178 TGTTGTGTATGGATGTAGCTTCTTG 132 25 0 40.00 60.458 4.00 2.00 5.458 179 GTGTTGTGTATGGATGTAGCTTCTTG 131 26 0 42.31 61.157 4.00 2.00 7.157 180 TGTTGTGTATGGATGTAGCTTCTT 132 24 0 37.50 57.904 4.00 4.00 6.096 181 GTGTTGTGTATGGATGTAGCTTCTT 131 25 0 40.00 58.712 4.00 4.00 6.288 182 TGTGTTGTGTATGGATGTAGCTTCTT 130 26 0 38.46 61.157 4.00 4.00 7.157 183 GTGTGTTGTGTATGGATGTAGCTTCTT 129 27 0 40.74 61.811 4.00 4.00 8.811 184 GTGTTGTGTATGGATGTAGCTTCT 131 24 0 41.67 57.400 4.00 2.00 6.600 185 TGTGTTGTGTATGGATGTAGCTTCT 130 25 0 40.00 60.002 4.00 2.00 5.002 186 GTGTGTTGTGTATGGATGTAGCTTCT 129 26 0 42.31 60.727 4.00 2.00 6.727 187 TGTGTTGTGTATGGATGTAGCTTC 130 24 0 41.67 59.091 4.00 4.00 4.909 188 GTGTGTTGTGTATGGATGTAGCTTC 129 25 0 44.00 59.880 4.00 4.00 5.120 189 TGTGTGTTGTGTATGGATGTAGCTTC 128 26 0 42.31 62.368 4.00 4.00 8.368 190 ATGTGTGTTGTGTATGGATGTAGCTTC 127 27 0 40.74 62.494 4.00 4.00 9.494 191 TGTGTTGTGTATGGATGTAGCTT 130 23 0 39.13 57.257 4.00 2.00 5.743 192 GTGTGTTGTGTATGGATGTAGCTT 129 24 0 41.67 58.137 4.00 2.00 5.863 193 TGTGTGTTGTGTATGGATGTAGCTT 128 25 0 40.00 60.742 4.00 2.00 5.742 194 ATGTGTGTTGTGTATGGATGTAGCTT 127 26 0 38.46 60.948 4.00 2.00 6.948 195 TGTGTGTTGTGTATGGATGTAGCT 128 24 0 41.67 59.493 4.00 4.00 4.507 196 ATGTGTGTTGTGTATGGATGTAGCT 127 25 0 40.00 59.772 4.00 4.00 5.228 197 CATGTGTGTTGTGTATGGATGTAGCT 126 26 0 42.31 62.266 4.00 4.00 8.266 198 ACATGTGTGTTGTGTATGGATGTAGCT 125 27 0 40.74 62.902 6.00 4.00 9.902 199 TGTGTGTTGTGTATGGATGTAGC 128 23 0 43.48 58.499 2.00 2.00 4.501 200 ATGTGTGTTGTGTATGGATGTAGC 127 24 0 41.67 58.842 2.00 2.00 5.158 201 CATGTGTGTTGTGTATGGATGTAGC 126 25 0 44.00 61.472 4.00 2.00 6.472 202 ACATGTGTGTTGTGTATGGATGTAGC 125 26 0 42.31 62.163 6.00 2.00 8.163 203 TACATGTGTGTTGTGTATGGATGTAGC 124 27 0 40.74 62.100 6.00 2.00 9.100 204 CATGTGTGTTGTGTATGGATGTAG 126 24 0 41.67 57.896 4.00 0.00 6.104 205 ACATGTGTGTTGTGTATGGATGTAG 125 25 0 40.00 58.761 6.00 0.00 6.239 206 TACATGTGTGTTGTGTATGGATGTAG 124 26 0 38.46 58.815 6.00 0.00 7.185 207 ATACATGTGTGTTGTGTATGGATGTAG 123 27 0 37.04 59.113 6.00 0.00 7.887 208 ACATGTGTGTTGTGTATGGATGTA 125 24 0 37.50 57.747 6.00 2.00 6.253 209 TACATGTGTGTTGTGTATGGATGTA 124 25 0 36.00 57.841 6.00 2.00 7.159 210 ATACATGTGTGTTGTGTATGGATGTA 123 26 0 34.62 58.200 6.00 2.00 7.800 211 TATACATGTGTGTTGTGTATGGATGTA 122 27 0 33.33 58.271 6.00 2.00 8.729 212 ACATGTGTGTTGTGTATGGATGT 125 23 0 39.13 57.646 6.00 0.00 5.354 213 TACATGTGTGTTGTGTATGGATGT 124 24 0 37.50 57.747 6.00 0.00 6.253 214 ATACATGTGTGTTGTGTATGGATGT 123 25 0 36.00 58.124 6.00 0.00 6.876 215 TATACATGTGTGTTGTGTATGGATGT 122 26 0 34.62 58.200 6.00 0.00 7.800 216 ATATACATGTGTGTTGTGTATGGATGT 121 27 0 33.33 58.526 6.00 0.00 8.474 217 ATACATGTGTGTTGTGTATGGATG 123 24 0 37.50 57.241 6.00 0.00 6.759 218 TATACATGTGTGTTGTGTATGGATG 122 25 0 36.00 57.352 6.00 0.00 7.648 219 ATATACATGTGTGTTGTGTATGGATG 121 26 0 34.62 57.730 6.00 0.00 8.270 220 CATATACATGTGTGTTGTGTATGGATG 120 27 0 37.04 60.230 6.00 0.00 7.230 221 CATATACATGTGTGTTGTGTATGGAT 120 26 0 34.62 57.730 6.00 2.00 8.270 222 GCATATACATGTGTGTTGTGTATGGAT 119 27 0 37.04 61.010 6.00 2.00 8.010 223 CATATACATGTGTGTTGTGTATGGA 120 25 0 36.00 57.352 6.00 0.00 7.648 224 GCATATACATGTGTGTTGTGTATGGA 119 26 0 38.46 60.811 6.00 1.00 6.811 225 GCATATACATGTGTGTTGTGTATGG 119 25 0 40.00 59.136 6.00 3.00 5.864 226 TGCATATACATGTGTGTTGTGTATGG 118 26 0 38.46 61.654 6.00 3.00 7.654 227 GTGCATATACATGTGTGTTGTGTATGG 117 27 0 40.74 62.312 6.00 3.00 9.312 228 TGCATATACATGTGTGTTGTGTATG 118 25 0 36.00 58.378 6.00 5.00 6.622 229 GTGCATATACATGTGTGTTGTGTATG 117 26 0 38.46 59.190 6.00 5.00 6.810 230 TGTGCATATACATGTGTGTTGTGTATG 116 27 0 37.04 61.688 6.00 5.00 8.688 231 TGTGCATATACATGTGTGTTGTGTAT 116 26 0 34.62 59.190 6.00 3.00 6.810 232 TTGTGCATATACATGTGTGTTGTGTAT 115 27 0 33.33 60.389 6.00 3.00 7.389 233 TGTGCATATACATGTGTGTTGTGTA 116 25 0 36.00 58.882 6.00 2.00 6.118 234 TTGTGCATATACATGTGTGTTGTGTA 115 26 0 34.62 60.156 6.00 2.00 6.156 235 ATTGTGCATATACATGTGTGTTGTGTA 114 27 0 33.33 60.389 6.00 2.00 7.389 236 TGTGCATATACATGTGTGTTGTGT 116 24 0 37.50 58.828 6.00 0.00 5.172 237 TTGTGCATATACATGTGTGTTGTGT 115 25 0 36.00 60.151 6.00 0.00 5.151 238 ATTGTGCATATACATGTGTGTTGTGT 114 26 0 34.62 60.393 6.00 0.00 6.393 239 TATTGTGCATATACATGTGTGTTGTGT 113 27 0 33.33 60.389 6.00 0.00 7.389 240 TGTGCATATACATGTGTGTTGTG 116 23 0 39.13 57.923 6.00 2.00 5.077 241 TTGTGCATATACATGTGTGTTGTG 115 24 0 37.50 59.345 6.00 2.00 4.655 242 ATTGTGCATATACATGTGTGTTGTG 114 25 0 36.00 59.637 6.00 2.00 5.363 243 TATTGTGCATATACATGTGTGTTGTG 113 26 0 34.62 59.659 6.00 2.00 6.341 244 GTATTGTGCATATACATGTGTGTTGTG 112 27 0 37.04 60.389 6.00 2.00 7.389 245 TATTGTGCATATACATGTGTGTTGT 113 25 0 32.00 57.073 6.00 2.00 7.927 246 GTATTGTGCATATACATGTGTGTTGT 112 26 0 34.62 57.913 6.00 2.00 8.087 247 GGTATTGTGCATATACATGTGTGTTGT 111 27 0 37.04 61.034 6.00 2.00 8.034 248 GTATTGTGCATATACATGTGTGTTG 112 25 0 36.00 57.073 6.00 4.00 7.927 249 GGTATTGTGCATATACATGTGTGTTG 111 26 0 38.46 60.346 6.00 4.00 6.346 250 TGGTATTGTGCATATACATGTGTGTTG 110 27 0 37.04 62.716 6.00 4.00 9.716 251 GGTATTGTGCATATACATGTGTGTT 111 25 0 36.00 57.851 6.00 4.00 7.149 252 TGGTATTGTGCATATACATGTGTGTT 110 26 0 34.62 60.346 6.00 4.00 6.346 253 GTGGTATTGTGCATATACATGTGTGTT 109 27 0 37.04 61.034 6.00 4.00 8.034 254 TGGTATTGTGCATATACATGTGTGT 110 25 0 36.00 59.136 6.00 6.00 5.864 255 GTGGTATTGTGCATATACATGTGTGT 109 26 0 38.46 59.900 6.00 6.00 6.100 256 TGTGGTATTGTGCATATACATGTGTGT 108 27 0 37.04 62.312 6.00 6.00 9.312 257 TGGTATTGTGCATATACATGTGTG 110 24 0 37.50 58.315 6.00 4.00 5.685 258 GTGGTATTGTGCATATACATGTGTG 109 25 0 40.00 59.136 6.00 4.00 5.864 259 TGTGGTATTGTGCATATACATGTGTG 108 26 0 38.46 61.654 6.00 4.00 7.654 260 GTGTGGTATTGTGCATATACATGTGTG 107 27 0 40.74 62.312 6.00 4.00 9.312 261 TGTGGTATTGTGCATATACATGTGT 108 25 0 36.00 59.136 6.00 6.00 5.864 262 GTGTGGTATTGTGCATATACATGTGT 107 26 0 38.46 59.900 6.00 6.00 6.100 263 TGTGTGGTATTGTGCATATACATGTGT 106 27 0 37.04 62.312 6.00 6.00 9.312 264 TGTGGTATTGTGCATATACATGTG 108 24 0 37.50 58.315 6.00 4.00 5.685 265 GTGTGGTATTGTGCATATACATGTG 107 25 0 40.00 59.136 6.00 4.00 5.864 266 TGTGTGGTATTGTGCATATACATGTG 106 26 0 38.46 61.654 6.00 4.00 7.654 267 ATGTGTGGTATTGTGCATATACATGTG 105 27 0 37.04 61.814 6.00 4.00 8.814 268 TGTGTGGTATTGTGCATATACATGT 106 25 0 36.00 59.136 6.00 6.00 5.864 269 ATGTGTGGTATTGTGCATATACATGT 105 26 0 34.62 59.420 6.00 6.00 6.580 270 CATGTGTGGTATTGTGCATATACATGT 104 27 0 37.04 61.814 6.00 6.00 8.814 271 TGTGTGGTATTGTGCATATACATG 106 24 0 37.50 58.315 6.00 6.00 5.685 272 ATGTGTGGTATTGTGCATATACATG 105 25 0 36.00 58.650 6.00 6.00 6.350 273 CATGTGTGGTATTGTGCATATACATG 104 26 0 38.46 61.147 6.00 6.00 7.147 274 ACATGTGTGGTATTGTGCATATACATG 103 27 0 37.04 61.814 6.00 6.00 8.814 275 CATGTGTGGTATTGTGCATATACAT 104 25 0 36.00 58.650 6.00 4.00 6.350 276 ACATGTGTGGTATTGTGCATATACAT 103 26 0 34.62 59.420 6.00 4.00 6.580 277 AACATGTGTGGTATTGTGCATATACAT 102 27 0 33.33 60.562 6.00 4.00 7.562 278 CATGTGTGGTATTGTGCATATACA 104 24 0 37.50 58.315 6.00 6.00 5.685 279 ACATGTGTGGTATTGTGCATATACA 103 25 0 36.00 59.136 6.00 6.00 5.864 280 AACATGTGTGGTATTGTGCATATACA 102 26 0 34.62 60.346 6.00 6.00 6.346 281 AAACATGTGTGGTATTGTGCATATACA 101 27 0 33.33 61.445 6.00 6.00 8.445 282 AACATGTGTGGTATTGTGCATATAC 102 25 0 36.00 57.851 6.00 6.00 7.149 283 AAACATGTGTGGTATTGTGCATATAC 101 26 0 34.62 59.095 6.00 6.00 6.905 284 TAAACATGTGTGGTATTGTGCATATAC 100 27 0 33.33 59.133 6.00 6.00 7.867 285 AACATGTGTGGTATTGTGCATATA 102 24 0 33.33 57.002 6.00 4.00 6.998 286 AAACATGTGTGGTATTGTGCATATA 101 25 0 32.00 58.331 6.00 4.00 6.669 287 TAAACATGTGTGGTATTGTGCATATA 100 26 0 30.77 58.395 6.00 4.00 7.605 288 TTAAACATGTGTGGTATTGTGCATATA 99 27 0 29.63 59.560 6.00 4.00 7.440 289 AAACATGTGTGGTATTGTGCATAT 101 24 0 33.33 58.262 6.00 4.00 5.738 290 TAAACATGTGTGGTATTGTGCATAT 100 25 0 32.00 58.331 6.00 4.00 6.669 291 TTAAACATGTGTGGTATTGTGCATAT 99 26 0 30.77 59.536 6.00 4.00 6.464 292 GTTAAACATGTGTGGTATTGTGCATAT 98 27 0 33.33 60.227 6.00 4.00 7.227 293 AAACATGTGTGGTATTGTGCATA 101 23 0 34.78 57.903 6.00 4.00 5.097 294 TAAACATGTGTGGTATTGTGCATA 100 24 0 33.33 57.988 6.00 4.00 6.012 295 TTAAACATGTGTGGTATTGTGCATA 99 25 0 32.00 59.268 6.00 4.00 5.732 296 GTTAAACATGTGTGGTATTGTGCATA 98 26 0 34.62 60.001 6.00 4.00 6.001 297 TGTTAAACATGTGTGGTATTGTGCATA 97 27 0 33.33 62.329 7.00 4.00 9.329 298 AAACATGTGTGGTATTGTGCAT 101 22 0 36.36 57.811 6.00 2.00 4.189 299 TAAACATGTGTGGTATTGTGCAT 100 23 0 34.78 57.903 6.00 2.00 5.097 300 TTAAACATGTGTGGTATTGTGCAT 99 24 0 33.33 59.233 6.00 2.00 4.767 301 GTTAAACATGTGTGGTATTGTGCAT 98 25 0 36.00 59.991 6.00 2.00 5.009 302 TGTTAAACATGTGTGGTATTGTGCAT 97 26 0 34.62 62.397 7.00 2.00 8.397 303 AAACATGTGTGGTATTGTGCA 101 21 0 38.10 57.391 6.00 4.00 3.609 304 TAAACATGTGTGGTATTGTGCA 100 22 0 36.36 57.504 6.00 4.00 4.496 305 TTAAACATGTGTGGTATTGTGCA 99 23 0 34.78 58.925 6.00 4.00 4.075 306 GTTAAACATGTGTGGTATTGTGCA 98 24 0 37.50 59.733 6.00 4.00 4.267 307 TGTTAAACATGTGTGGTATTGTGCA 97 25 0 36.00 62.264 7.00 4.00 7.264 308 GTTAAACATGTGTGGTATTGTGC 98 23 0 39.13 57.060 6.00 2.00 5.940 309 TGTTAAACATGTGTGGTATTGTGC 97 24 0 37.50 59.733 7.00 2.00 4.267 310 CTGTTAAACATGTGTGGTATTGTGC 96 25 0 40.00 60.599 7.00 2.00 5.599 311 TCTGTTAAACATGTGTGGTATTGTGC 95 26 0 38.46 62.185 7.00 2.00 8.185 312 GTCTGTTAAACATGTGTGGTATTGTGC 94 27 0 40.74 62.806 7.00 2.00 9.806 313 CTGTTAAACATGTGTGGTATTGTG 96 24 0 37.50 57.140 7.00 0.00 6.860 314 TCTGTTAAACATGTGTGGTATTGTG 95 25 0 36.00 58.912 7.00 0.00 6.088 315 GTCTGTTAAACATGTGTGGTATTGTG 94 26 0 38.46 59.680 7.00 0.00 6.320 316 TGTCTGTTAAACATGTGTGGTATTGTG 93 27 0 37.04 62.089 7.00 0.00 9.089 317 GTCTGTTAAACATGTGTGGTATTGT 94 25 0 36.00 57.143 7.00 0.00 7.857 318 TGTCTGTTAAACATGTGTGGTATTGT 93 26 0 34.62 59.680 7.00 0.00 6.320 319 GTGTCTGTTAAACATGTGTGGTATTGT 92 27 0 37.04 60.396 7.00 0.00 7.396 320 TGTCTGTTAAACATGTGTGGTATTG 93 25 0 36.00 58.912 7.00 0.00 6.088 321 GTGTCTGTTAAACATGTGTGGTATTG 92 26 0 38.46 59.680 7.00 0.00 6.320 322 TGTGTCTGTTAAACATGTGTGGTATTG 91 27 0 37.04 62.089 7.00 0.00 9.089 323 GTGTCTGTTAAACATGTGTGGTATT 92 25 0 36.00 57.143 7.00 1.00 7.857 324 TGTGTCTGTTAAACATGTGTGGTATT 91 26 0 34.62 59.680 7.00 1.00 6.320 325 GTGTGTCTGTTAAACATGTGTGGTATT 90 27 0 37.04 60.396 7.00 1.00 7.396 326 TGTGTCTGTTAAACATGTGTGGTAT 91 25 0 36.00 58.424 7.00 2.00 6.576 327 GTGTGTCTGTTAAACATGTGTGGTAT 90 26 0 38.46 59.220 7.00 2.00 6.780 328 GGTGTGTCTGTTAAACATGTGTGGTAT 89 27 0 40.74 62.286 7.00 2.00 9.286 329 TGTGTCTGTTAAACATGTGTGGTA 91 24 0 37.50 58.069 7.00 2.00 5.931 330 GTGTGTCTGTTAAACATGTGTGGTA 90 25 0 40.00 58.919 7.00 2.00 6.081 331 GGTGTGTCTGTTAAACATGTGTGGTA 89 26 0 42.31 62.150 7.00 2.00 8.150 332 TGTGTCTGTTAAACATGTGTGGT 91 23 0 39.13 57.983 7.00 0.00 5.017 333 GTGTGTCTGTTAAACATGTGTGGT 90 24 0 41.67 58.868 7.00 0.00 5.132 334 GGTGTGTCTGTTAAACATGTGTGGT 89 25 0 44.00 62.217 7.00 0.00 7.217 335 TGTGTCTGTTAAACATGTGTGG 91 22 0 40.91 57.024 7.00 2.00 4.976 336 GTGTGTCTGTTAAACATGTGTGG 90 23 0 43.48 57.983 7.00 2.00 5.017 337 GGTGTGTCTGTTAAACATGTGTGG 89 24 0 45.83 61.509 7.00 2.00 5.509 338 GGTGTGTCTGTTAAACATGTGTG 89 23 0 43.48 57.983 7.00 4.00 5.017 339 GGGTGTGTCTGTTAAACATGTGTG 88 24 0 45.83 61.509 7.00 4.00 5.509 340 GGGTGTGTCTGTTAAACATGTGT 88 23 0 43.48 58.808 7.00 6.00 4.192 341 TGGGTGTGTCTGTTAAACATGTGT 87 24 0 41.67 61.509 7.00 6.00 5.509 342 GTGGGTGTGTCTGTTAAACATGTGT 86 25 0 44.00 62.217 7.00 6.00 7.217 343 GGGTGTGTCTGTTAAACATGTG 88 22 0 45.45 57.912 7.00 4.00 4.088 344 TGGGTGTGTCTGTTAAACATGTG 87 23 0 43.48 60.746 7.00 4.00 3.746 345 GTGGGTGTGTCTGTTAAACATGTG 86 24 0 45.83 61.509 7.00 4.00 5.509 346 TGGGTGTGTCTGTTAAACATGT 87 22 0 40.91 57.912 7.00 6.00 4.088 347 GTGGGTGTGTCTGTTAAACATGT 86 23 0 43.48 58.808 7.00 6.00 4.192 348 TGTGGGTGTGTCTGTTAAACATGT 85 24 0 41.67 61.509 7.00 6.00 5.509 349 TTGTGGGTGTGTCTGTTAAACATGT 84 25 0 40.00 62.648 7.00 6.00 7.648 350 GTGGGTGTGTCTGTTAAACATG 86 22 0 45.45 57.912 7.00 5.00 4.088 351 TGTGGGTGTGTCTGTTAAACATG 85 23 0 43.48 60.746 7.00 5.00 3.746 352 TTGTGGGTGTGTCTGTTAAACATG 84 24 0 41.67 61.967 7.00 5.00 5.967 353 CTTGTGGGTGTGTCTGTTAAACATG 83 25 0 44.00 62.749 7.00 5.00 7.749 354 TGTGGGTGTGTCTGTTAAACAT 85 22 0 40.91 57.912 7.00 5.00 4.088 355 TTGTGGGTGTGTCTGTTAAACAT 84 23 0 39.13 59.319 7.00 5.00 3.681 356 CTTGTGGGTGTGTCTGTTAAACAT 83 24 0 41.67 60.239 7.00 5.00 4.239 357 TCTTGTGGGTGTGTCTGTTAAACAT 82 25 0 40.00 61.898 7.00 5.00 6.898 358 GTCTTGTGGGTGTGTCTGTTAAACAT 81 26 0 42.31 62.553 7.00 5.00 8.553 359 TGTGGGTGTGTCTGTTAAACA 85 21 0 42.86 57.489 7.00 7.00 3.511 360 TTGTGGGTGTGTCTGTTAAACA 84 22 0 40.91 58.997 7.00 7.00 3.003 361 CTTGTGGGTGTGTCTGTTAAACA 83 23 0 43.48 59.981 7.00 7.00 3.019 362 TCTTGTGGGTGTGTCTGTTAAACA 82 24 0 41.67 61.733 7.00 7.00 5.733 363 GTCTTGTGGGTGTGTCTGTTAAACA 81 25 0 44.00 62.427 7.00 7.00 7.427 364 CTTGTGGGTGTGTCTGTTAAAC 83 22 0 45.45 57.176 5.00 5.00 4.824 365 TCTTGTGGGTGTGTCTGTTAAAC 82 23 0 43.48 59.063 5.00 5.00 3.937 366 GTCTTGTGGGTGTGTCTGTTAAAC 81 24 0 45.83 59.875 5.00 5.00 4.125 367 TGTCTTGTGGGTGTGTCTGTTAAAC 80 25 0 44.00 62.427 5.00 5.00 7.427 368 TCTTGTGGGTGTGTCTGTTAAA 82 22 0 40.91 58.187 4.00 3.00 3.813 369 GTCTTGTGGGTGTGTCTGTTAAA 81 23 0 43.48 59.063 4.00 3.00 3.937 370 TGTCTTGTGGGTGTGTCTGTTAAA 80 24 0 41.67 61.733 4.00 3.00 5.733 371 GTGTCTTGTGGGTGTGTCTGTTAAA 79 25 0 44.00 62.427 4.00 3.00 7.427 372 GTCTTGTGGGTGTGTCTGTTAA 81 22 0 45.45 57.635 4.00 4.00 4.365 373 TGTCTTGTGGGTGTGTCTGTTAA 80 23 0 43.48 60.493 4.00 4.00 3.493 374 GTGTCTTGTGGGTGTGTCTGTTAA 79 24 0 45.83 61.270 4.00 4.00 5.270 375 TGTCTTGTGGGTGTGTCTGTTA 80 22 0 45.45 59.108 2.00 2.00 2.892 376 GTGTCTTGTGGGTGTGTCTGTTA 79 23 0 47.83 59.984 2.00 2.00 3.016 377 TGTGTCTTGTGGGTGTGTCTGTTA 78 24 0 45.83 62.734 2.00 2.00 6.734 378 TGTCTTGTGGGTGTGTCTGTT 80 21 0 47.62 59.059 1.00 0.00 1.941 379 GTGTCTTGTGGGTGTGTCTGTT 79 22 0 50.00 59.972 1.00 0.00 2.028 380 TGTGTCTTGTGGGTGTGTCTGTT 78 23 0 47.83 62.831 1.00 0.00 5.831 381 TGTCTTGTGGGTGTGTCTGT 80 20 0 50.00 57.431 1.00 0.00 2.569 382 GTGTCTTGTGGGTGTGTCTGT 79 21 0 52.38 58.472 1.00 0.00 2.528 383 TGTGTCTTGTGGGTGTGTCTGT 78 22 0 50.00 61.549 1.00 0.00 3.549 384 GTGTCTTGTGGGTGTGTCTG 79 20 0 55.00 57.431 1.00 1.00 2.569 385 TGTGTCTTGTGGGTGTGTCTG 78 21 0 52.38 60.680 1.00 1.00 1.680 386 TGTGTCTTGTGGGTGTGTCT 78 20 0 50.00 57.431 1.00 0.00 2.569 387 TGTTGTGTATAAATGTTGTGTCTTCA 51 26 0 30.77 58.596 4.00 1.00 7.404 388 GTGTTGTGTATAAATGTTGTGTCTTCA 50 27 0 33.33 59.358 4.00 1.00 7.642 389 TGTGTTGTGTATAAATGTTGTGTCTTC 49 27 0 33.33 59.358 4.00 0.00 7.642 390 TGTGTTGTGTATAAATGTTGTGTCTT 49 26 0 30.77 57.700 4.00 0.00 8.300 391 ATGTGTTGTGTATAAATGTTGTGTCTT 48 27 0 29.63 58.041 4.00 0.00 8.959 392 CATATGTGTTGTGTATAAATGTTGTGT 45 27 0 29.63 57.112 6.00 0.00 9.888 393 ACATATGTGTTGTGTATAAATGTTGTG 44 27 0 29.63 57.112 8.00 0.00 9.888 394 CACATATGTGTTGTGTATAAATGTTGT 43 27 0 29.63 57.112 10.00 2.00 9.888 395 ACACATATGTGTTGTGTATAAATGTTG 42 27 0 29.63 57.112 12.00 0.00 9.888 396 CATGTGTGGCATTGTGTATACA 23 22 0 40.91 57.850 8.00 8.00 4.150 397 ACATGTGTGGCATTGTGTATACA 22 23 0 39.13 58.766 8.00 8.00 4.234 398 AACATGTGTGGCATTGTGTATACA 21 24 0 37.50 60.103 8.00 8.00 4.103 399 GAACATGTGTGGCATTGTGTATACA 20 25 0 40.00 61.810 8.00 8.00 6.810 400 AACATGTGTGGCATTGTGTATAC 21 23 0 39.13 57.373 6.00 6.00 5.627 401 GAACATGTGTGGCATTGTGTATAC 20 24 0 41.67 59.208 6.00 6.00 4.792 402 GAACATGTGTGGCATTGTGTATA 20 23 0 39.13 58.360 6.00 4.00 4.640 403 GAACATGTGTGGCATTGTGTAT 20 22 0 40.91 58.283 6.00 2.00 3.717 404 GAACATGTGTGGCATTGTGTA 20 21 0 42.86 57.880 6.00 2.00 3.120 405 GAACATGTGTGGCATTGTGT 20 20 0 45.00 57.775 6.00 0.00 2.225 primer3-2.3.6/test/primer_list_last/SP01-02.txt.for0000664000077300007730000000217012231156244020640 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGTTTCACACTTACGGAAATGG 9 22 0 40.91 59.898 6.00 4.00 2.102 1 TTTCACACTTACGGAAATGGA 11 21 0 38.10 58.134 6.00 6.00 2.866 2 GTTTCACACTTACGGAAATGGA 10 22 0 40.91 58.997 6.00 6.00 3.003 3 GCTGTTTCACACTTACGGAAA 7 21 0 42.86 57.947 4.00 3.00 3.053 4 GCTGTTTCACACTTACGGAAAT 7 22 0 40.91 58.314 4.00 4.00 3.686 5 CTGTTTCACACTTACGGAAATGG 8 23 0 43.48 60.784 6.00 4.00 3.784 6 GTTTCACACTTACGGAAATGG 10 21 0 42.86 57.142 6.00 4.00 3.858 7 GCTGTTTCACACTTACGGAAATG 7 23 0 43.48 60.918 6.00 6.00 3.918 8 TGTTTCACACTTACGGAAATGGA 9 23 0 39.13 61.623 6.00 6.00 4.623 9 CTGTTTCACACTTACGGAAATG 8 22 0 40.91 57.367 6.00 6.00 4.633 10 CTTAGCGTGCGTGGGGTC 141 18 0 66.67 62.798 3.00 1.00 4.798 11 CTGTTTCACACTTACGGAAATGGA 8 24 0 41.67 62.411 6.00 6.00 6.411 primer3-2.3.6/test/primer_list_last/SP01-06-with-repeat3.rev0000664000077300007730000003425112231156244022353 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 GTGCTTTGCGTTTTGGAAGT 125 20 0 45.00 60.292 3.00 1.00 0.292 1 GTGCTTTGCGTTTTGGAAG 125 19 0 47.37 59.454 3.00 0.00 1.546 2 TGCTTTGCGTTTTGGAAGT 124 19 0 42.11 59.454 3.00 1.00 1.546 3 TGCTTTGCGTTTTGGAAGTTA 124 21 0 38.10 60.764 3.00 2.00 1.764 4 GCTTTGCGTTTTGGAAGTTA 123 20 0 40.00 58.097 3.00 2.00 1.903 5 GCTTTGCGTTTTGGAAGTTAG 123 21 0 42.86 59.065 3.00 0.00 1.935 6 GAGCGTGCTTTGCGTTTT 129 18 0 50.00 60.142 4.00 0.00 2.142 7 GCTTTGCGTTTTGGAAGTTAGA 123 22 0 40.91 60.748 3.00 1.00 2.748 8 AGCGTGCTTTGCGTTTTG 128 18 0 50.00 61.131 4.00 0.00 3.131 9 TGCTTTGCGTTTTGGAAG 124 18 0 44.44 58.546 3.00 0.00 3.454 10 GTGCTTTGCGTTTTGGAAGTTA 125 22 0 40.91 61.484 3.00 2.00 3.484 11 TGCTTTGCGTTTTGGAAGTTAG 124 22 0 40.91 61.593 3.00 0.00 3.593 12 GTGCTTTGCGTTTTGGAA 125 18 0 44.44 58.389 2.00 2.00 3.611 13 TGCGTTTTGGAAGTTAGACTTTG 119 23 0 39.13 60.658 6.00 2.00 3.658 14 CTTTGCGTTTTGGAAGTTAGA 122 21 0 38.10 57.272 3.00 1.00 3.728 15 TTTGCGTTTTGGAAGTTAGA 121 20 0 35.00 56.191 3.00 1.00 3.809 16 CTTTGCGTTTTGGAAGTTAGAC 122 22 0 40.91 58.127 3.00 2.00 3.873 17 GCGTTTTGGAAGTTAGACTTTG 118 22 0 40.91 58.127 6.00 2.00 3.873 18 TTTGCGTTTTGGAAGTTAGAC 121 21 0 38.10 57.124 3.00 2.00 3.876 19 CGTGCTTTGCGTTTTGGA 126 18 0 50.00 61.944 2.00 0.00 3.944 20 CGTGCTTTGCGTTTTGGAAG 126 20 0 50.00 63.967 3.00 0.00 3.967 21 GAGCGTGCTTTGCGTTTTG 129 19 0 52.63 62.977 4.00 0.00 3.977 22 CGTGCTTTGCGTTTTGGAA 126 19 0 47.37 63.187 2.00 2.00 4.187 23 TTGCGTTTTGGAAGTTAGAC 120 20 0 40.00 55.619 3.00 2.00 4.381 24 GCTTTGCGTTTTGGAAGTTAGAC 123 23 0 43.48 61.438 3.00 2.00 4.438 25 GGAGCGTGCTTTGCGTTT 130 18 0 55.56 62.465 4.00 0.00 4.465 26 CTTTGCGTTTTGGAAGTTAG 122 20 0 40.00 55.385 3.00 0.00 4.615 27 GGAGCGTGCTTTGCGTTTT 130 19 0 52.63 63.650 4.00 0.00 4.650 28 GATCGGGAGCGTGCTTTG 135 18 0 61.11 62.907 4.00 0.00 4.907 29 GTGCTTTGCGTTTTGGAAGTTAG 125 23 0 43.48 62.256 3.00 0.00 5.256 30 GCTTTGCGTTTTGGAAGT 123 18 0 44.44 56.483 3.00 1.00 5.517 31 AGCGTGCTTTGCGTTTTGG 128 19 0 52.63 64.584 4.00 0.00 5.584 32 CGTGCTTTGCGTTTTGGAAGT 126 21 0 47.62 64.590 3.00 1.00 5.590 33 TTGCGTTTTGGAAGTTAGACTTTG 120 24 0 37.50 61.761 6.00 2.00 5.761 34 GCGTGCTTTGCGTTTTGG 127 18 0 55.56 63.814 2.00 0.00 5.814 35 TGCTTTGCGTTTTGGAAGTTAGA 124 23 0 39.13 63.165 3.00 1.00 6.165 36 GGAGCGTGCTTTGCGTTTTG 130 20 0 55.00 66.221 4.00 0.00 6.221 37 GAGCGTGCTTTGCGTTTTGG 129 20 0 55.00 66.221 4.00 0.00 6.221 38 AGCGTGCTTTGCGTTTTGGA 128 20 0 50.00 66.221 4.00 0.00 6.221 39 TTGCGTTTTGGAAGTTAGA 120 19 0 36.84 54.570 3.00 1.00 6.430 40 CTTTGACAACAGACTCATACAGTGA 101 25 0 40.00 58.507 5.00 2.00 6.493 41 TTTGACAACAGACTCATACAGTGA 100 24 0 37.50 57.479 5.00 2.00 6.521 42 GCGTGCTTTGCGTTTTGGAA 127 20 0 50.00 66.535 2.00 2.00 6.535 43 GCGTGCTTTGCGTTTTGGA 127 19 0 52.63 65.553 2.00 0.00 6.553 44 CGTTTTGGAAGTTAGACTTTG 117 21 0 38.10 54.425 6.00 2.00 6.575 45 ACTTTGACAACAGACTCATACAGTGA 102 26 0 38.46 59.320 5.00 2.00 6.680 46 GGGAGCGTGCTTTGCGTT 131 18 0 61.11 64.759 4.00 0.00 6.759 47 GGGAGCGTGCTTTGCGTTTT 131 20 0 55.00 66.761 4.00 0.00 6.761 48 ATCGGGAGCGTGCTTTGC 134 18 0 61.11 64.764 4.00 2.00 6.764 49 GGGAGCGTGCTTTGCGTTT 131 19 0 57.89 65.814 4.00 0.00 6.814 50 CTTTGCGTTTTGGAAGTTA 122 19 0 36.84 54.178 3.00 2.00 6.822 51 TTTGCGTTTTGGAAGTTAG 121 19 0 36.84 54.178 3.00 0.00 6.822 52 TGCGTTTTGGAAGTTAGAC 119 19 0 42.11 53.939 3.00 2.00 7.061 53 TTGACAACAGACTCATACAGTGA 99 23 0 39.13 55.925 5.00 2.00 7.075 54 GATCGGGAGCGTGCTTTGC 135 19 0 63.16 66.488 4.00 2.00 7.488 55 GTGCTTTGCGTTTTGGAAGTTAGA 125 24 0 41.67 63.758 3.00 1.00 7.758 56 TGCTTTGCGTTTTGGAAGTTAGAC 124 24 0 41.67 63.758 3.00 2.00 7.758 57 TTTGCGTTTTGGAAGTTAGACTTTG 121 25 0 36.00 62.766 6.00 2.00 7.766 58 GACTTTGACAACAGACTCATACAGTGA 103 27 0 40.74 60.988 5.00 2.00 7.988 59 AGCGTGCTTTGCGTTTTGGAA 128 21 0 47.62 67.128 4.00 2.00 8.128 60 GCGTGCTTTGCGTTTTGGAAG 127 21 0 52.38 67.128 3.00 0.00 8.128 61 AGACTTTGACAACAGACTCATACAG 104 25 0 40.00 56.820 7.00 1.00 8.180 62 AGACTTTGACAACAGACTCATACA 104 24 0 37.50 55.732 7.00 0.00 8.268 63 GACTTTGACAACAGACTCATACAG 103 24 0 41.67 55.732 5.00 1.00 8.268 64 CGTGCTTTGCGTTTTGGAAGTTA 126 23 0 43.48 65.376 3.00 2.00 8.376 65 GACTTTGACAACAGACTCATACA 103 23 0 39.13 54.538 5.00 0.00 8.462 66 TTAGACTTTGACAACAGACTCATACA 106 26 0 34.62 57.298 7.00 1.00 8.702 67 GAGCGTGCTTTGCGTTTTGGA 129 21 0 52.38 67.764 4.00 0.00 8.764 68 TTAGACTTTGACAACAGACTCATACAG 106 27 0 37.04 58.234 7.00 1.00 8.766 69 GTTAGACTTTGACAACAGACTCATACA 107 27 0 37.04 58.107 7.00 1.00 8.893 70 TAGACTTTGACAACAGACTCATACAG 105 26 0 38.46 56.940 7.00 1.00 9.060 71 TAGACTTTGACAACAGACTCATACA 105 25 0 36.00 55.897 7.00 0.00 9.103 72 TTTGCGTTTTGGAAGTTA 121 18 0 33.33 52.848 3.00 2.00 9.152 73 TGCGTTTTGGAAGTTAGA 119 18 0 38.89 52.751 3.00 1.00 9.249 74 ACTTTGACAACAGACTCATACAG 102 23 0 39.13 53.733 5.00 1.00 9.267 75 GTGCTTTGCGTTTTGGAAGTTAGAC 125 25 0 44.00 64.312 3.00 2.00 9.312 76 CTTTGACAACAGACTCATACAG 101 22 0 40.91 52.603 5.00 1.00 9.397 77 CTTTGCGTTTTGGAAGTTAGACTTTG 122 26 0 38.46 63.414 6.00 2.00 9.414 78 ATCGGGAGCGTGCTTTGCGT 134 20 0 60.00 69.470 4.00 0.00 9.470 79 ACTTTGACAACAGACTCATACA 102 22 0 36.36 52.399 5.00 0.00 9.601 80 GCGTGCTTTGCGTTTTGGAAGT 127 22 0 50.00 67.608 3.00 1.00 9.608 81 AGACTTTGACAACAGACTCA 104 20 0 40.00 50.384 7.00 1.00 9.616 82 TTGCGTTTTGGAAGTTAG 120 18 0 38.89 52.384 3.00 0.00 9.616 83 AGCGTGCTTTGCGTTTTGGAAG 128 22 0 50.00 67.671 4.00 0.00 9.671 84 AGACTTTGACAACAGACTCAT 104 21 0 38.10 51.260 7.00 2.00 9.740 85 CGGGAGCGTGCTTTGCGTTT 132 20 0 60.00 69.763 4.00 0.00 9.763 86 CTTTGACAACAGACTCATACA 101 21 0 38.10 51.152 5.00 0.00 9.848 87 TTTGACAACAGACTCATACAG 100 21 0 38.10 51.152 5.00 1.00 9.848 88 CGTGCTTTGCGTTTTGGAAGTTAG 126 24 0 45.83 65.955 3.00 0.00 9.955 89 CGGGAGCGTGCTTTGCGTT 132 19 0 63.16 68.983 4.00 0.00 9.983 90 ATCGGGAGCGTGCTTTGCG 134 19 0 63.16 69.025 4.00 2.00 10.025 91 GGGAGCGTGCTTTGCGTTTTG 131 21 0 57.14 69.108 4.00 0.00 10.108 92 GGAGCGTGCTTTGCGTTTTGG 130 21 0 57.14 69.108 4.00 0.00 10.108 93 CGGGAGCGTGCTTTGCGT 132 18 0 66.67 68.116 4.00 0.00 10.116 94 AGACTTTGACAACAGACTCATAC 104 23 0 39.13 52.792 7.00 0.00 10.208 95 GACTTTGACAACAGACTCAT 103 20 0 40.00 49.674 5.00 2.00 10.326 96 TTAGACTTTGACAACAGACTCATAC 106 25 0 36.00 54.653 7.00 1.00 10.347 97 TAGACTTTGACAACAGACTCAT 105 22 0 36.36 51.625 7.00 2.00 10.375 98 AGACTTTGACAACAGACTCATA 104 22 0 36.36 51.625 7.00 2.00 10.375 99 TTTGACAACAGACTCATACA 100 20 0 35.00 49.540 5.00 0.00 10.460 100 TCGGGAGCGTGCTTTGCGTT 133 20 0 60.00 70.467 4.00 0.00 10.467 101 GATCGGGAGCGTGCTTTGCG 135 20 0 65.00 70.520 4.00 2.00 10.520 102 GAGCGTGCTTTGCGTTTTGGAA 129 22 0 50.00 68.571 4.00 2.00 10.571 103 GACTTTGACAACAGACTCATAC 103 22 0 40.91 51.414 5.00 0.00 10.586 104 TCGGGAGCGTGCTTTGCGT 133 19 0 63.16 69.707 4.00 0.00 10.707 105 GTTTTGGAAGTTAGACTTTG 116 20 0 35.00 49.271 6.00 2.00 10.729 106 GACTTTGACAACAGACTCATA 103 21 0 38.10 50.125 5.00 2.00 10.875 107 TAGACTTTGACAACAGACTCATAC 105 24 0 37.50 53.072 7.00 0.00 10.928 108 TTGACAACAGACTCATACAG 99 20 0 40.00 49.008 5.00 1.00 10.992 109 TAGACTTTGACAACAGACTCATA 105 23 0 34.78 51.962 7.00 2.00 11.038 110 AGCGTGCTTTGCGTTTTGGAAGT 128 23 0 47.83 68.114 4.00 1.00 11.114 111 ATCGGGAGCGTGCTTTGCGTT 134 21 0 57.14 70.204 4.00 0.00 11.204 112 TCGGGAGCGTGCTTTGCG 133 18 0 66.67 69.247 4.00 2.00 11.247 113 GCGTTTTGGAAGTTAGAC 118 18 0 44.44 50.670 3.00 2.00 11.330 114 CGGGAGCGTGCTTTGCGTTTT 132 21 0 57.14 70.468 4.00 0.00 11.468 115 GATCGGGAGCGTGCTTTGCGT 135 21 0 61.90 70.899 4.00 0.00 11.899 116 ACTTTGACAACAGACTCATAC 102 21 0 38.10 49.101 5.00 0.00 11.899 117 GAGCGTGCTTTGCGTTTTGGAAG 129 23 0 52.17 69.042 4.00 0.00 12.042 118 GCGTGCTTTGCGTTTTGGAAGTTA 127 24 0 45.83 68.126 3.00 2.00 12.126 119 TCGGGAGCGTGCTTTGCGTTT 133 21 0 57.14 71.153 4.00 0.00 12.153 120 CGTGCTTTGCGTTTTGGAAGTTAGA 126 25 0 44.00 67.289 3.00 1.00 12.289 121 ACTTTGACAACAGACTCATA 102 20 0 35.00 47.663 5.00 2.00 12.337 122 CTTTGACAACAGACTCATAC 101 20 0 40.00 47.663 5.00 0.00 12.337 123 GACTTTGACAACAGACTCA 103 19 0 42.11 48.639 5.00 1.00 12.361 124 GGAGCGTGCTTTGCGTTTTGGA 130 22 0 54.55 70.490 4.00 0.00 12.490 125 ATCGGGAGCGTGCTTTGCGTTT 134 22 0 54.55 70.870 4.00 0.00 12.870 126 GCTTTGCGTTTTGGAAGTTAGACTTTG 123 27 0 40.74 66.026 6.00 2.00 13.026 127 TTTTGGAAGTTAGACTTTG 115 19 0 31.58 47.928 6.00 2.00 13.072 128 GAGCGTGCTTTGCGTTTTGGAAGT 129 24 0 50.00 69.429 4.00 1.00 13.429 129 GATCGGGAGCGTGCTTTGCGTT 135 22 0 59.09 71.545 4.00 0.00 13.545 130 AGCGTGCTTTGCGTTTTGGAAGTTA 128 25 0 44.00 68.576 4.00 2.00 13.576 131 GCGTGCTTTGCGTTTTGGAAGTTAG 127 25 0 48.00 68.576 3.00 0.00 13.576 132 GGGAGCGTGCTTTGCGTTTTGG 131 22 0 59.09 71.695 4.00 0.00 13.695 133 CGTGCTTTGCGTTTTGGAAGTTAGAC 126 26 0 46.15 67.709 3.00 2.00 13.709 134 TCGGGAGCGTGCTTTGCGTTTT 133 22 0 54.55 71.774 4.00 0.00 13.774 135 TTGACAACAGACTCATACA 99 19 0 36.84 47.159 5.00 0.00 13.841 136 ACTTTGACAACAGACTCAT 102 19 0 36.84 47.081 5.00 2.00 13.919 137 GGAGCGTGCTTTGCGTTTTGGAA 130 23 0 52.17 71.131 4.00 2.00 14.131 138 ATCGGGAGCGTGCTTTGCGTTTT 134 23 0 52.17 71.476 4.00 0.00 14.476 139 CGGGAGCGTGCTTTGCGTTTTG 132 22 0 59.09 72.594 4.00 0.00 14.594 140 CTTTGACAACAGACTCATA 101 19 0 36.84 46.089 5.00 2.00 14.911 141 AGCGTGCTTTGCGTTTTGGAAGTTAG 128 26 0 46.15 68.993 4.00 0.00 14.993 142 GATCGGGAGCGTGCTTTGCGTTT 135 23 0 56.52 72.132 4.00 0.00 15.132 143 TTTGACAACAGACTCATAC 100 19 0 36.84 45.794 5.00 0.00 15.206 144 GGAGCGTGCTTTGCGTTTTGGAAG 130 24 0 54.17 71.481 4.00 0.00 15.481 145 GAGCGTGCTTTGCGTTTTGGAAGTTA 129 26 0 46.15 69.786 4.00 2.00 15.786 146 GCGTGCTTTGCGTTTTGGAAGTTAGA 127 26 0 46.15 69.786 3.00 1.00 15.786 147 GGGAGCGTGCTTTGCGTTTTGGA 131 23 0 56.52 72.940 4.00 0.00 15.940 148 ACTTTGACAACAGACTCA 102 18 0 38.89 45.825 5.00 1.00 16.175 149 TTTGGAAGTTAGACTTTG 114 18 0 33.33 45.649 6.00 2.00 16.351 150 CTTTGACAACAGACTCAT 101 18 0 38.89 45.402 5.00 2.00 16.598 151 GATCGGGAGCGTGCTTTGCGTTTT 135 24 0 54.17 72.668 4.00 0.00 16.668 152 GGAGCGTGCTTTGCGTTTTGGAAGT 130 25 0 52.00 71.774 4.00 1.00 16.774 153 TCGGGAGCGTGCTTTGCGTTTTG 133 23 0 56.52 73.813 4.00 0.00 16.813 154 GCGTGCTTTGCGTTTTGGAAGTTAGAC 127 27 0 48.15 70.114 3.00 2.00 17.114 155 GAGCGTGCTTTGCGTTTTGGAAGTTAG 129 27 0 48.15 70.152 4.00 0.00 17.152 156 AGCGTGCTTTGCGTTTTGGAAGTTAGA 128 27 0 44.44 70.152 4.00 1.00 17.152 157 ATCGGGAGCGTGCTTTGCGTTTTG 134 24 0 54.17 73.434 4.00 0.00 17.434 158 GGGAGCGTGCTTTGCGTTTTGGAA 131 24 0 54.17 73.443 4.00 2.00 17.443 159 CGGGAGCGTGCTTTGCGTTTTGG 132 23 0 60.87 74.875 4.00 0.00 17.875 160 TTTGACAACAGACTCATA 100 18 0 33.33 44.031 5.00 2.00 17.969 161 GGGAGCGTGCTTTGCGTTTTGGAAG 131 25 0 56.00 73.692 4.00 0.00 18.692 162 TTGACAACAGACTCATAC 99 18 0 38.89 43.056 5.00 0.00 18.944 163 GGAGCGTGCTTTGCGTTTTGGAAGTTA 130 27 0 48.15 71.956 4.00 2.00 18.956 164 GATCGGGAGCGTGCTTTGCGTTTTG 135 25 0 56.00 74.552 4.00 0.00 19.552 165 GGGAGCGTGCTTTGCGTTTTGGAAGT 131 26 0 53.85 73.906 4.00 1.00 19.906 166 TCGGGAGCGTGCTTTGCGTTTTGG 133 24 0 58.33 75.980 4.00 0.00 19.980 167 CGGGAGCGTGCTTTGCGTTTTGGA 132 24 0 58.33 75.980 4.00 0.00 19.980 168 ATCGGGAGCGTGCTTTGCGTTTTGG 134 25 0 56.00 75.528 4.00 0.00 20.528 169 CGGGAGCGTGCTTTGCGTTTTGGAA 132 25 0 56.00 76.331 4.00 2.00 21.331 170 TCGGGAGCGTGCTTTGCGTTTTGGA 133 25 0 56.00 77.032 4.00 0.00 22.032 171 CGGGAGCGTGCTTTGCGTTTTGGAAG 132 26 0 57.69 76.468 4.00 0.00 22.468 172 GATCGGGAGCGTGCTTTGCGTTTTGG 135 26 0 57.69 76.548 4.00 0.00 22.548 173 ATCGGGAGCGTGCTTTGCGTTTTGGA 134 26 0 53.85 76.548 4.00 0.00 22.548 174 TCGGGAGCGTGCTTTGCGTTTTGGAA 133 26 0 53.85 77.333 4.00 2.00 23.333 175 CGGGAGCGTGCTTTGCGTTTTGGAAGT 132 27 0 55.56 76.595 4.00 1.00 23.595 176 ATCGGGAGCGTGCTTTGCGTTTTGGAA 134 27 0 51.85 76.856 4.00 2.00 23.856 177 TCGGGAGCGTGCTTTGCGTTTTGGAAG 133 27 0 55.56 77.432 4.00 0.00 24.432 178 GATCGGGAGCGTGCTTTGCGTTTTGGA 135 27 0 55.56 77.522 4.00 0.00 24.522 primer3-2.3.6/test/primer_list_last/SP04-07.txt.rev0000664000077300007730000012433112231156244020662 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TTCTTTCCGTGGCTCTCCTA 281 20 0 50.00 59.948 2.00 2.00 0.052 1 TCCCTTGGCCACATTCTTAG 141 20 0 50.00 60.066 6.00 3.00 0.066 2 CTTGGCCACATTCTTAGGGA 138 20 0 50.00 60.066 6.00 2.00 0.066 3 TGGCCACATTCTTAGGGAAC 136 20 0 50.00 59.933 6.00 0.00 0.067 4 GGCCACATTCTTAGGGAACA 135 20 0 50.00 59.933 4.00 0.00 0.067 5 CCTCATTTTCCCACAGCTGT 223 20 0 50.00 60.111 8.00 8.00 0.111 6 CTTCAGTGTGTACATGGCGG 114 20 0 55.00 60.175 6.00 1.00 0.175 7 ATTCTTTCCGTGGCTCTCCT 282 20 0 50.00 60.212 2.00 0.00 0.212 8 GCAGTTACACCACCCCATTC 298 20 0 55.00 60.240 3.00 0.00 0.240 9 TCTCCTAGCTGGGTTTGTGG 268 20 0 55.00 60.246 6.00 0.00 0.246 10 CCTAGCTGGGTTTGTGGCTA 265 20 0 55.00 60.262 6.00 2.00 0.262 11 TGTGGCTAGTCATGGAGGTG 253 20 0 55.00 59.701 6.00 0.00 0.299 12 AAGAGCCTCATTTTCCCACA 228 20 0 45.00 59.670 5.00 0.00 0.330 13 TACACCACCCCATTCTTTCC 293 20 0 50.00 59.647 2.00 0.00 0.353 14 GGGTTTGTGGCTAGTCATGG 258 20 0 55.00 60.375 6.00 2.00 0.375 15 TCTTTCCGTGGCTCTCCTAG 280 20 0 55.00 59.569 4.00 4.00 0.431 16 TGGGTTTGTGGCTAGTCATG 259 20 0 50.00 59.566 6.00 4.00 0.434 17 TTCCCTTGGCCACATTCTTA 142 20 0 45.00 60.439 6.00 2.00 0.439 18 TTGGCCACATTCTTAGGGAA 137 20 0 45.00 60.439 6.00 2.00 0.439 19 AGCTGGGTTTGTGGCTAGTC 262 20 0 55.00 59.357 4.00 4.00 0.643 20 CTAGTCATGGAGGTGCCCTG 248 20 0 60.00 60.668 4.00 2.00 0.668 21 GTGGCTAGTCATGGAGGTGC 252 20 0 60.00 60.688 6.00 2.00 0.688 22 ATTCCCTTGGCCACATTCTT 143 20 0 45.00 60.693 6.00 0.00 0.693 23 GCCCTGAAGAGCCTCATTTT 234 20 0 50.00 60.713 5.00 1.00 0.713 24 TGAAGAGCCTCATTTTCCCA 230 20 0 45.00 60.713 5.00 0.00 0.713 25 AGAGCCTCATTTTCCCACAG 227 20 0 50.00 59.284 5.00 1.00 0.716 26 GGAAGTGTGTGGTACACCCA 202 20 0 55.00 59.283 6.00 6.00 0.717 27 GTGGCTCTCCTAGCTGGGTT 273 20 0 60.00 60.786 6.00 2.00 0.786 28 GGGAAGGTCTTCAGTGCATT 160 20 0 50.00 59.141 6.00 4.00 0.859 29 AAGGTCTTCAGTGCATTCCC 157 20 0 50.00 59.141 4.00 2.00 0.859 30 GGTCTTCAGTGCATTCCCTT 155 20 0 50.00 59.141 4.00 2.00 0.859 31 TGGCTCTCCTAGCTGGGTT 272 19 0 57.89 59.958 6.00 2.00 1.042 32 TTCTTTCCGTGGCTCTCCT 281 19 0 52.63 59.935 2.00 0.00 1.065 33 TTCCGTGGCTCTCCTAGCT 277 19 0 57.89 60.104 4.00 4.00 1.104 34 CCTCATTTTCCCACAGCTGTA 223 21 0 47.62 60.118 8.00 6.00 1.118 35 TCCCACAGCTGTAGGAAGTG 215 20 0 55.00 58.879 12.00 2.00 1.121 36 AGGGAAGGTCTTCAGTGCATT 161 21 0 47.62 60.125 6.00 4.00 1.125 37 AAGGTCTTCAGTGCATTCCCT 157 21 0 47.62 60.125 4.00 4.00 1.125 38 AGGTCTTCAGTGCATTCCCTT 156 21 0 47.62 60.125 4.00 2.00 1.125 39 CCCTGAAGAGCCTCATTTTC 233 20 0 50.00 58.861 5.00 1.00 1.139 40 CCTGAAGAGCCTCATTTTCC 232 20 0 50.00 58.861 5.00 0.00 1.139 41 CTGAAGAGCCTCATTTTCCC 231 20 0 50.00 58.861 5.00 0.00 1.139 42 TTAGGGAAGGTCTTCAGTGCA 163 21 0 47.62 59.861 6.00 4.00 1.139 43 AGCCTCATTTTCCCACAGCT 225 20 0 50.00 61.153 4.00 4.00 1.153 44 TCTTCAGTGCATTCCCTTGG 153 20 0 50.00 61.176 4.00 2.00 1.176 45 TGCAGTTACACCACCCCAT 299 19 0 52.63 59.822 4.00 2.00 1.178 46 GGAAGTGTGTGGTACACCCAC 202 21 0 57.14 60.189 8.00 8.00 1.189 47 TGGCTAGTCATGGAGGTGC 251 19 0 57.89 59.803 6.00 2.00 1.197 48 GGCCACATTCTTAGGGAACAT 135 21 0 47.62 60.201 4.00 2.00 1.201 49 CCATTCTTTCCGTGGCTCT 284 19 0 52.63 60.203 3.00 0.00 1.203 50 ATGGAGGTGCCCTGAAGAG 242 19 0 57.89 60.207 4.00 2.00 1.207 51 AGCCTCATTTTCCCACAGC 225 19 0 52.63 60.211 3.00 2.00 1.211 52 GCCTCATTTTCCCACAGCT 224 19 0 52.63 60.211 4.00 4.00 1.211 53 ATTCTTTCCGTGGCTCTCCTA 282 21 0 47.62 60.214 2.00 2.00 1.214 54 TGCAGTTACACCACCCCATT 299 20 0 50.00 61.216 4.00 1.00 1.216 55 TCCCACAGCTGTAGGAAGTGT 215 21 0 52.38 59.777 12.00 1.00 1.223 56 GCTGGGTTTGTGGCTAGTCA 261 20 0 55.00 61.236 6.00 6.00 1.236 57 AGGGAAGGTCTTCAGTGCAT 161 20 0 50.00 58.744 6.00 2.00 1.256 58 AGGTCTTCAGTGCATTCCCT 156 20 0 50.00 58.744 4.00 4.00 1.256 59 CCTAGCTGGGTTTGTGGCT 265 19 0 57.89 60.262 6.00 0.00 1.262 60 TGGCTCTCCTAGCTGGGTTT 272 20 0 55.00 61.275 6.00 0.00 1.275 61 GGCTCTCCTAGCTGGGTTTG 271 20 0 60.00 61.275 6.00 2.00 1.275 62 TTTGTGGCTAGTCATGGAGG 255 20 0 50.00 58.722 6.00 0.00 1.278 63 GAAGAGCCTCATTTTCCCAC 229 20 0 50.00 58.717 5.00 0.00 1.283 64 TGTGTGGTACACCCACAATG 197 20 0 50.00 58.674 8.00 3.00 1.326 65 AGGAAGTGTGTGGTACACCCA 203 21 0 52.38 60.328 6.00 6.00 1.328 66 TAGTCATGGAGGTGCCCTG 247 19 0 57.89 59.641 4.00 2.00 1.359 67 GTGCCCTGAAGAGCCTCAT 236 19 0 57.89 60.365 5.00 5.00 1.365 68 GTGTGTGGTACACCCACAATG 198 21 0 52.38 59.632 8.00 3.00 1.368 69 TGTGTGGTACACCCACAATGT 197 21 0 47.62 59.632 8.00 5.00 1.368 70 ACACCACCCCATTCTTTCC 292 19 0 52.63 59.621 2.00 0.00 1.379 71 GGTTTGTGGCTAGTCATGGAG 257 21 0 52.38 59.607 6.00 0.00 1.393 72 GTTTGTGGCTAGTCATGGAGG 256 21 0 52.38 59.607 6.00 0.00 1.393 73 TTTGTGGCTAGTCATGGAGGT 255 21 0 47.62 59.607 6.00 0.00 1.393 74 CTTTCCGTGGCTCTCCTAGC 279 20 0 60.00 61.407 4.00 2.00 1.407 75 TTTCCGTGGCTCTCCTAGCT 278 20 0 55.00 61.407 4.00 4.00 1.407 76 GGTTTGTGGCTAGTCATGGA 257 20 0 50.00 58.570 6.00 2.00 1.430 77 TTCCCTTGGCCACATTCTT 142 19 0 47.37 60.445 6.00 0.00 1.445 78 GCTCTCCTAGCTGGGTTTGT 270 20 0 55.00 58.551 6.00 0.00 1.449 79 TTTCCGTGGCTCTCCTAGC 278 19 0 57.89 60.489 4.00 2.00 1.489 80 CTGGGTTTGTGGCTAGTCATG 260 21 0 52.38 60.556 6.00 4.00 1.556 81 TAGGGAAGGTCTTCAGTGCA 162 20 0 50.00 58.442 6.00 4.00 1.558 82 GTGGCTCTCCTAGCTGGGT 273 19 0 63.16 59.420 6.00 4.00 1.580 83 GCTAGTCATGGAGGTGCCCT 249 20 0 60.00 61.585 4.00 4.00 1.585 84 TAGCTGGGTTTGTGGCTAGTC 263 21 0 52.38 59.392 4.00 4.00 1.608 85 AAGAGCCTCATTTTCCCACAG 228 21 0 47.62 60.615 5.00 1.00 1.615 86 GCCCTGAAGAGCCTCATTT 234 19 0 52.63 59.376 5.00 3.00 1.624 87 TAGTCATGGAGGTGCCCTGA 247 20 0 55.00 61.630 4.00 4.00 1.630 88 GGCTAGTCATGGAGGTGCC 250 19 0 63.16 60.639 4.00 2.00 1.639 89 GCTAGTCATGGAGGTGCCC 249 19 0 63.16 60.639 4.00 2.00 1.639 90 ATTCCCTTGGCCACATTCT 143 19 0 47.37 59.351 6.00 1.00 1.649 91 GAAGTGTGTGGTACACCCACA 201 21 0 52.38 59.347 8.00 8.00 1.653 92 ATTCCCTTGGCCACATTCTTA 143 21 0 42.86 60.678 6.00 2.00 1.678 93 GTGCCCTGAAGAGCCTCATT 236 20 0 55.00 61.702 5.00 5.00 1.702 94 GTCTTCAGTGCATTCCCTTG 154 20 0 50.00 58.287 4.00 2.00 1.713 95 TCTTCAGTGTGTACATGGCG 115 20 0 50.00 58.276 6.00 2.00 1.724 96 CCCTTGGCCACATTCTTAGG 140 20 0 55.00 61.732 6.00 2.00 1.732 97 CCTTGGCCACATTCTTAGGG 139 20 0 55.00 61.732 6.00 0.00 1.732 98 CCCACAGCTGTAGGAAGTGTG 214 21 0 57.14 60.749 10.00 3.00 1.749 99 ATTCTTTCCGTGGCTCTCC 282 19 0 52.63 59.228 2.00 0.00 1.772 100 CCTCATTTTCCCACAGCTG 223 19 0 52.63 59.226 6.00 6.00 1.774 101 CTTCAGTGCATTCCCTTGG 152 19 0 52.63 59.226 4.00 2.00 1.774 102 TTGTGGCTAGTCATGGAGGT 254 20 0 50.00 58.158 6.00 0.00 1.842 103 TTCTTTCCGTGGCTCTCCTAG 281 21 0 52.38 60.870 4.00 4.00 1.870 104 TGCCCTGAAGAGCCTCATT 235 19 0 52.63 60.898 5.00 5.00 1.898 105 TTCAGTGTGTACATGGCGG 113 19 0 52.63 59.097 6.00 1.00 1.903 106 TCCCTTGGCCACATTCTTA 141 19 0 47.37 59.061 6.00 2.00 1.939 107 TTGGCCACATTCTTAGGGA 137 19 0 47.37 59.061 6.00 2.00 1.939 108 TGGCCACATTCTTAGGGAA 136 19 0 47.37 59.061 6.00 2.00 1.939 109 TTACACCACCCCATTCTTTCC 294 21 0 47.62 60.953 2.00 0.00 1.953 110 GGGAAGGTCTTCAGTGCATTC 160 21 0 52.38 61.014 6.00 2.00 2.014 111 GGAAGGTCTTCAGTGCATTCC 159 21 0 52.38 61.014 6.00 4.00 2.014 112 GAAGGTCTTCAGTGCATTCCC 158 21 0 52.38 61.014 6.00 2.00 2.014 113 CCATTCTTTCCGTGGCTCTC 284 20 0 55.00 62.053 3.00 0.00 2.053 114 CATTCTTTCCGTGGCTCTCC 283 20 0 55.00 62.053 2.00 0.00 2.053 115 AGGTGCCCTGAAGAGCCT 238 18 0 61.11 59.945 4.00 3.00 2.055 116 GAGCCTCATTTTCCCACAGC 226 20 0 55.00 62.069 5.00 2.00 2.069 117 GTGCCCTGAAGAGCCTCA 236 18 0 61.11 60.071 5.00 4.00 2.071 118 CTCCTAGCTGGGTTTGTGGC 267 20 0 60.00 62.090 6.00 2.00 2.090 119 TCCTAGCTGGGTTTGTGGCT 266 20 0 55.00 62.090 6.00 0.00 2.090 120 TGGAGGTGCCCTGAAGAG 241 18 0 61.11 59.903 4.00 2.00 2.097 121 TTGTGGCTAGTCATGGAGGTG 254 21 0 52.38 61.100 6.00 0.00 2.100 122 CACCCCATTCTTTCCGTG 288 18 0 55.56 59.886 3.00 0.00 2.114 123 ATTAGGGAAGGTCTTCAGTGCA 164 22 0 45.45 60.130 6.00 4.00 2.130 124 TTAGGGAAGGTCTTCAGTGCAT 163 22 0 45.45 60.130 6.00 2.00 2.130 125 TAGGGAAGGTCTTCAGTGCATT 162 22 0 45.45 60.130 6.00 4.00 2.130 126 CAGTTACACCACCCCATTCTTT 297 22 0 45.45 60.144 3.00 0.00 2.144 127 CCTAGCTGGGTTTGTGGCTAG 265 21 0 57.14 61.155 6.00 4.00 2.155 128 CATTCCCTTGGCCACATTCT 144 20 0 50.00 62.168 6.00 1.00 2.168 129 CTCTCCTAGCTGGGTTTGTGG 269 21 0 57.14 61.173 6.00 0.00 2.173 130 CAGTTACACCACCCCATTCTT 297 21 0 47.62 58.826 3.00 0.00 2.174 131 GCAGTTACACCACCCCATTCT 298 21 0 52.38 61.176 3.00 0.00 2.176 132 AAGTGTGTGGTACACCCACAA 200 21 0 47.62 58.817 8.00 6.00 2.183 133 TGCCCTGAAGAGCCTCATTT 235 20 0 50.00 62.185 5.00 3.00 2.185 134 TCCTAGCTGGGTTTGTGGC 266 19 0 57.89 61.192 6.00 2.00 2.192 135 TGTGGTACACCCACAATGTCTT 195 22 0 45.45 60.193 6.00 1.00 2.193 136 CATGGAGGTGCCCTGAAG 243 18 0 61.11 60.197 4.00 2.00 2.197 137 TAGGGAAGGTCTTCAGTGCAT 162 21 0 47.62 58.803 6.00 2.00 2.197 138 CCCACAGCTGTAGGAAGTGT 214 20 0 55.00 57.801 10.00 1.00 2.199 139 TTGGCCACATTCTTAGGGAAC 137 21 0 47.62 61.214 6.00 0.00 2.214 140 TGTGGTACACCCACAATGTCT 195 21 0 47.62 58.765 6.00 1.00 2.235 141 CATTCCCTTGGCCACATTC 144 19 0 52.63 61.265 6.00 0.00 2.265 142 CAGTGTGTACATGGCGGC 111 18 0 61.11 59.685 6.00 3.00 2.315 143 CTAGCTGGGTTTGTGGCTAGTC 264 22 0 54.55 60.315 5.00 4.00 2.315 144 GCTCTCCTAGCTGGGTTTGTG 270 21 0 57.14 61.316 6.00 0.00 2.316 145 CCGTGGCTCTCCTAGCTG 275 18 0 66.67 59.676 4.00 2.00 2.324 146 CGTGGCTCTCCTAGCTGG 274 18 0 66.67 59.676 4.00 4.00 2.324 147 TAGGAAGTGTGTGGTACACCCA 204 22 0 50.00 60.326 6.00 6.00 2.326 148 CTGGGTTTGTGGCTAGTCAT 260 20 0 50.00 57.671 6.00 4.00 2.329 149 GTGTGGTACACCCACAATGTCT 196 22 0 50.00 59.670 6.00 1.00 2.330 150 TTCCCTTGGCCACATTCTTAG 142 21 0 47.62 61.331 6.00 3.00 2.331 151 CTTGGCCACATTCTTAGGGAA 138 21 0 47.62 61.331 6.00 2.00 2.331 152 CTAGCTGGGTTTGTGGCTAG 264 20 0 55.00 57.665 5.00 4.00 2.335 153 ATCTTCAGTGTGTACATGGCG 116 21 0 47.62 58.664 6.00 2.00 2.336 154 AGTCATGGAGGTGCCCTG 246 18 0 61.11 59.612 4.00 2.00 2.388 155 GTGTGGTACACCCACAATGTC 196 21 0 52.38 58.609 6.00 3.00 2.391 156 TGTGGTACACCCACAATGTC 195 20 0 50.00 57.608 6.00 3.00 2.392 157 GTTTGTGGCTAGTCATGGAGGT 256 22 0 50.00 60.424 6.00 0.00 2.424 158 GCTGGGTTTGTGGCTAGTCAT 261 21 0 52.38 61.441 6.00 4.00 2.441 159 CTAGCTGGGTTTGTGGCTAGT 264 21 0 52.38 58.548 5.00 2.00 2.452 160 CTCTCCTAGCTGGGTTTGTG 269 20 0 55.00 57.547 6.00 0.00 2.453 161 TCTTTCCGTGGCTCTCCTA 280 19 0 52.63 58.528 2.00 2.00 2.472 162 GCCACATTCTTAGGGAACATC 134 21 0 47.62 58.517 3.00 1.00 2.483 163 TAGCTGGGTTTGTGGCTAGT 263 20 0 50.00 57.512 4.00 2.00 2.488 164 TGAAGAGCCTCATTTTCCCAC 230 21 0 47.62 61.500 5.00 0.00 2.500 165 GAAGAGCCTCATTTTCCCACA 229 21 0 47.62 61.500 5.00 0.00 2.500 166 GTGTGTGGTACACCCACAATGT 198 22 0 50.00 60.510 8.00 5.00 2.510 167 GCCACATTCTTAGGGAACATCT 134 22 0 45.45 59.487 3.00 2.00 2.513 168 TGCAGTTACACCACCCCA 299 18 0 55.56 59.486 4.00 0.00 2.514 169 GGCTCTCCTAGCTGGGTTT 271 19 0 57.89 58.486 6.00 0.00 2.514 170 ACCACCCCATTCTTTCCGT 290 19 0 52.63 61.532 2.00 0.00 2.532 171 TGCCCTGAAGAGCCTCAT 235 18 0 55.56 59.452 5.00 5.00 2.548 172 CTGAAGAGCCTCATTTTCCCA 231 21 0 47.62 61.618 5.00 0.00 2.618 173 CATTCTTTCCGTGGCTCTC 283 19 0 52.63 58.373 2.00 0.00 2.627 174 AGGGAAGGTCTTCAGTGCA 161 19 0 52.63 58.364 6.00 4.00 2.636 175 TTACACCACCCCATTCTTTC 294 20 0 45.00 57.364 2.00 0.00 2.636 176 CAGTTACACCACCCCATTCT 297 20 0 50.00 57.357 3.00 0.00 2.643 177 AGTGTGTGGTACACCCACAATG 199 22 0 50.00 60.644 8.00 4.00 2.644 178 GTCATGGAGGTGCCCTGA 245 18 0 61.11 60.651 4.00 4.00 2.651 179 TCCGTGGCTCTCCTAGCTG 276 19 0 63.16 61.652 4.00 2.00 2.652 180 AGGAAGTGTGTGGTACACCC 203 20 0 55.00 57.315 5.00 5.00 2.685 181 AGTCATGGAGGTGCCCTGA 246 19 0 57.89 61.688 4.00 4.00 2.688 182 GCTGGGTTTGTGGCTAGTC 261 19 0 57.89 58.301 4.00 4.00 2.699 183 CTCCTAGCTGGGTTTGTGG 267 19 0 57.89 58.300 6.00 0.00 2.700 184 CATTCCCTTGGCCACATT 144 18 0 50.00 59.296 6.00 1.00 2.704 185 GGAAGGTCTTCAGTGCATTC 159 20 0 50.00 57.288 6.00 2.00 2.712 186 GAAGGTCTTCAGTGCATTCC 158 20 0 50.00 57.288 6.00 3.00 2.712 187 ACCACCCCATTCTTTCCG 290 18 0 55.56 60.715 2.00 2.00 2.715 188 CCACCCCATTCTTTCCGT 289 18 0 55.56 60.715 2.00 0.00 2.715 189 ACCCCATTCTTTCCGTGG 287 18 0 55.56 60.715 3.00 2.00 2.715 190 GTTACACCACCCCATTCTTTC 295 21 0 47.62 58.280 3.00 0.00 2.720 191 GCAGTTACACCACCCCATT 298 19 0 52.63 58.273 3.00 1.00 2.727 192 AGTTACACCACCCCATTCTTTC 296 22 0 45.45 59.259 3.00 0.00 2.741 193 CCTAGCTGGGTTTGTGGC 265 18 0 61.11 59.241 6.00 2.00 2.759 194 GAAGTGTGTGGTACACCCACAA 201 22 0 50.00 60.765 8.00 6.00 2.765 195 AAGTGTGTGGTACACCCACA 200 20 0 50.00 57.229 8.00 8.00 2.771 196 AGTGTGTGGTACACCCACAA 199 20 0 50.00 57.229 8.00 6.00 2.771 197 TCAGTGTGTACATGGCGGC 112 19 0 57.89 61.775 6.00 3.00 2.775 198 TCAGTGCATTCCCTTGGC 150 18 0 55.56 60.784 4.00 2.00 2.784 199 AGAGCCTCATTTTCCCACA 227 19 0 47.37 58.216 5.00 0.00 2.784 200 GAGCCTCATTTTCCCACAG 226 19 0 52.63 58.216 5.00 1.00 2.784 201 CCACAGCTGTAGGAAGTGTGTG 213 22 0 54.55 60.796 8.00 1.00 2.796 202 CACAGCTGTAGGAAGTGTGTGG 212 22 0 54.55 60.796 8.00 0.00 2.796 203 GCCTCATTTTCCCACAGC 224 18 0 55.56 59.170 2.00 2.00 2.830 204 CCATTCTTTCCGTGGCTC 284 18 0 55.56 59.166 3.00 1.00 2.834 205 AAGTGTGTGGTACACCCACAAT 200 22 0 45.45 59.161 8.00 8.00 2.839 206 CCCTTGGCCACATTCTTAG 140 19 0 52.63 58.159 6.00 3.00 2.841 207 CCTTGGCCACATTCTTAGG 139 19 0 52.63 58.159 6.00 2.00 2.841 208 CTTGGCCACATTCTTAGGG 138 19 0 52.63 58.159 6.00 0.00 2.841 209 ATGGAGGTGCCCTGAAGA 242 18 0 55.56 59.129 4.00 0.00 2.871 210 GAGGTGCCCTGAAGAGCCT 239 19 0 63.16 61.885 4.00 3.00 2.885 211 AGGTGCCCTGAAGAGCCTC 238 19 0 63.16 61.885 5.00 5.00 2.885 212 TTCCGTGGCTCTCCTAGCTG 277 20 0 60.00 62.904 4.00 2.00 2.904 213 GGAGGTGCCCTGAAGAGC 240 18 0 66.67 60.924 4.00 4.00 2.924 214 GAGGTGCCCTGAAGAGCC 239 18 0 66.67 60.924 4.00 2.00 2.924 215 GGTGCCCTGAAGAGCCTC 237 18 0 66.67 60.924 5.00 5.00 2.924 216 TTCCGTGGCTCTCCTAGC 277 18 0 61.11 59.063 4.00 2.00 2.937 217 GGTCTTCAGTGCATTCCCTTG 155 21 0 52.38 61.957 4.00 2.00 2.957 218 GTCTTCAGTGCATTCCCTTGG 154 21 0 52.38 61.957 4.00 2.00 2.957 219 TTCCCTTGGCCACATTCT 142 18 0 50.00 59.009 6.00 1.00 2.991 220 TCCCTTGGCCACATTCTT 141 18 0 50.00 59.009 6.00 0.00 2.991 221 AGTCATGGAGGTGCCCTGAA 246 20 0 55.00 62.996 4.00 2.00 2.996 222 GTCATGGAGGTGCCCTGAAG 245 20 0 60.00 62.996 4.00 2.00 2.996 223 CCCATTCTTTCCGTGGCT 285 18 0 55.56 61.004 3.00 0.00 3.004 224 GTGGCTCTCCTAGCTGGGTTT 273 21 0 57.14 62.011 6.00 0.00 3.011 225 GGCTCTCCTAGCTGGGTTTGT 271 21 0 57.14 62.011 6.00 0.00 3.011 226 CCTCATTTTCCCACAGCTGTAG 223 22 0 50.00 61.023 8.00 8.00 3.023 227 TCCTAGCTGGGTTTGTGGCTA 266 21 0 52.38 62.023 6.00 2.00 3.023 228 ATTCTTTCCGTGGCTCTCCTAG 282 22 0 50.00 61.079 4.00 4.00 3.079 229 GTCATGGAGGTGCCCTGAA 245 19 0 57.89 62.091 4.00 2.00 3.091 230 TCTTCAGTGTGTACATGGCGG 115 21 0 52.38 62.092 6.00 1.00 3.092 231 TTCTTTCCGTGGCTCTCC 281 18 0 55.56 58.878 2.00 0.00 3.122 232 AGCTGGGTTTGTGGCTAGTCA 262 21 0 52.38 62.124 6.00 6.00 3.124 233 CCACAGCTGTAGGAAGTGTGT 213 21 0 52.38 57.875 8.00 1.00 3.125 234 ACAGCTGTAGGAAGTGTGTGG 211 21 0 52.38 57.875 8.00 0.00 3.125 235 CAGCTGTAGGAAGTGTGTGGT 210 21 0 52.38 57.875 6.00 0.00 3.125 236 TATTAGGGAAGGTCTTCAGTGCA 165 23 0 43.48 60.136 6.00 4.00 3.136 237 AGGAAGTGTGTGGTACACCCAC 203 22 0 54.55 61.149 8.00 8.00 3.149 238 TTCAGTGCATTCCCTTGGC 151 19 0 52.63 62.155 4.00 2.00 3.155 239 TGTGGTACACCCACAATGTCTTA 195 23 0 43.48 60.196 6.00 2.00 3.196 240 ACAGCTGTAGGAAGTGTGTGGT 211 22 0 50.00 58.799 8.00 0.00 3.201 241 GGGTTTGTGGCTAGTCATGGA 258 21 0 52.38 62.203 6.00 2.00 3.203 242 TGAAGAGCCTCATTTTCCC 230 19 0 47.37 57.791 5.00 0.00 3.209 243 GAAGAGCCTCATTTTCCCA 229 19 0 47.37 57.791 5.00 0.00 3.209 244 TCATGGAGGTGCCCTGAAG 244 19 0 57.89 62.218 4.00 2.00 3.218 245 CATGGAGGTGCCCTGAAGA 243 19 0 57.89 62.218 4.00 0.00 3.218 246 TCATGGAGGTGCCCTGAA 244 18 0 55.56 61.229 4.00 2.00 3.229 247 CTTTCCGTGGCTCTCCTAGCT 279 21 0 57.14 62.244 4.00 4.00 3.244 248 CCCTGAAGAGCCTCATTTTCC 233 21 0 52.38 62.304 5.00 0.00 3.304 249 CCTGAAGAGCCTCATTTTCCC 232 21 0 52.38 62.304 5.00 0.00 3.304 250 CACCACCCCATTCTTTCC 291 18 0 55.56 58.690 2.00 0.00 3.310 251 AGTGTGTGGTACACCCACAAT 199 21 0 47.62 57.686 8.00 8.00 3.314 252 CTAGTCATGGAGGTGCCCT 248 19 0 57.89 57.663 4.00 4.00 3.337 253 TCCGTGGCTCTCCTAGCT 276 18 0 61.11 58.643 4.00 4.00 3.357 254 AAGGTCTTCAGTGCATTCCCTT 157 22 0 45.45 61.364 4.00 3.00 3.364 255 GGGAAGGTCTTCAGTGCAT 160 19 0 52.63 57.630 6.00 2.00 3.370 256 AGGTCTTCAGTGCATTCCC 156 19 0 52.63 57.630 4.00 2.00 3.370 257 GGTCTTCAGTGCATTCCCT 155 19 0 52.63 57.630 4.00 4.00 3.370 258 CTTTCCGTGGCTCTCCTAG 279 19 0 57.89 57.628 4.00 4.00 3.372 259 ATTAGGGAAGGTCTTCAGTGCAT 164 23 0 43.48 60.373 6.00 2.00 3.373 260 GCTCTCCTAGCTGGGTTTG 270 19 0 57.89 57.626 6.00 2.00 3.374 261 GCCCTGAAGAGCCTCATTTTC 234 21 0 52.38 62.443 5.00 1.00 3.443 262 GGTACACCCACAATGTCTTAGC 192 22 0 50.00 58.513 5.00 2.00 3.487 263 CTCATTTTCCCACAGCTGTAG 222 21 0 47.62 57.499 8.00 8.00 3.501 264 ATTCCCTTGGCCACATTCTTAG 143 22 0 45.45 61.515 6.00 3.00 3.515 265 GTGGCTCTCCTAGCTGGG 273 18 0 66.67 58.482 6.00 6.00 3.518 266 TGGCTCTCCTAGCTGGGT 272 18 0 61.11 58.482 6.00 4.00 3.518 267 CTAGTCATGGAGGTGCCCTGA 248 21 0 57.14 62.530 4.00 4.00 3.530 268 GGTACACCCACAATGTCTTAGCT 192 23 0 47.83 59.468 5.00 4.00 3.532 269 CATCTTCAGTGTGTACATGGCG 117 22 0 50.00 61.537 6.00 2.00 3.537 270 CCCACAGCTGTAGGAAGTGTGT 214 22 0 54.55 61.541 10.00 1.00 3.541 271 TCTTTCCGTGGCTCTCCT 280 18 0 55.56 58.453 2.00 0.00 3.547 272 AGTTACACCACCCCATTCTTT 296 21 0 42.86 57.448 3.00 0.00 3.552 273 CCCACAATGTCTTAGCTTTTG 186 21 0 42.86 57.442 5.00 3.00 3.558 274 TAGGAAGTGTGTGGTACACCC 204 21 0 52.38 57.434 5.00 5.00 3.566 275 ATTAGGGAAGGTCTTCAGTGC 164 21 0 47.62 57.417 6.00 2.00 3.583 276 TGTGTGGTACACCCACAATGTC 197 22 0 50.00 61.591 8.00 3.00 3.591 277 AGCTGGGTTTGTGGCTAGT 262 19 0 52.63 57.400 4.00 2.00 3.600 278 GTAGGAAGTGTGTGGTACACCC 205 22 0 54.55 58.361 5.00 5.00 3.639 279 TGGCCACATTCTTAGGGAACA 136 21 0 47.62 62.668 6.00 0.00 3.668 280 GTTACACCACCCCATTCTTTCC 295 22 0 50.00 61.686 3.00 0.00 3.686 281 CACCCACAATGTCTTAGCTTTT 188 22 0 40.91 58.314 5.00 1.00 3.686 282 ACCCACAATGTCTTAGCTTTTG 187 22 0 40.91 58.314 5.00 3.00 3.686 283 CCCACAATGTCTTAGCTTTTGT 186 22 0 40.91 58.314 5.00 5.00 3.686 284 GCCACATTCTTAGGGAACATCTT 134 23 0 43.48 60.707 4.00 4.00 3.707 285 TCTTCAGTGCATTCCCTTG 153 19 0 47.37 57.292 4.00 2.00 3.708 286 ATTCCCTTGGCCACATTC 143 18 0 50.00 58.274 6.00 0.00 3.726 287 GTGGTACACCCACAATGTCTT 194 21 0 47.62 57.273 5.00 1.00 3.727 288 CTGGGTTTGTGGCTAGTCA 260 19 0 52.63 57.225 6.00 6.00 3.775 289 AGTGTGTACATGGCGGCG 110 18 0 61.11 61.777 6.00 3.00 3.777 290 TTGTGGCTAGTCATGGAGG 254 19 0 52.63 57.156 6.00 0.00 3.844 291 CCCATTCTTTCCGTGGCTC 285 19 0 57.89 62.863 3.00 1.00 3.863 292 CCTAGCTGGGTTTGTGGCTAGT 265 22 0 54.55 61.865 6.00 2.00 3.865 293 GCATTCCCTTGGCCACAT 145 18 0 55.56 61.869 6.00 2.00 3.869 294 ACACCCACAATGTCTTAGCTTTT 189 23 0 39.13 59.123 5.00 1.00 3.877 295 ACCCACAATGTCTTAGCTTTTGT 187 23 0 39.13 59.123 5.00 5.00 3.877 296 CATTCTTTCCGTGGCTCTCCT 283 21 0 52.38 62.881 2.00 0.00 3.881 297 AGGGAAGGTCTTCAGTGCATTC 161 22 0 50.00 61.884 6.00 2.00 3.884 298 GAAGGTCTTCAGTGCATTCCCT 158 22 0 50.00 61.884 6.00 4.00 3.884 299 TAGTCATGGAGGTGCCCTGAA 247 21 0 52.38 62.891 4.00 2.00 3.891 300 GTGCCCTGAAGAGCCTCATTT 236 21 0 52.38 62.898 5.00 3.00 3.898 301 AGAGCCTCATTTTCCCACAGC 227 21 0 52.38 62.898 5.00 2.00 3.898 302 GAGCCTCATTTTCCCACAGCT 226 21 0 52.38 62.898 5.00 4.00 3.898 303 TTCAGTGCATTCCCTTGG 151 18 0 50.00 58.095 4.00 2.00 3.905 304 CTCCTAGCTGGGTTTGTGGCT 267 21 0 57.14 62.907 6.00 0.00 3.907 305 CACCCACAATGTCTTAGCTTTTG 188 23 0 43.48 60.909 5.00 3.00 3.909 306 GGCCACATTCTTAGGGAACATC 135 22 0 50.00 61.913 4.00 1.00 3.913 307 AACATCTTCAGTGTGTACATGGC 119 23 0 43.48 59.046 6.00 2.00 3.954 308 GGCCACATTCTTAGGGAAC 135 19 0 52.63 57.030 4.00 0.00 3.970 309 CCACATTCTTAGGGAACATCTTC 133 23 0 43.48 59.014 6.00 6.00 3.986 310 GAAGTGTGTGGTACACCCACAAT 201 23 0 47.83 60.995 8.00 8.00 3.995 311 GTGTGGTACACCCACAATGTCTT 196 23 0 47.83 60.995 6.00 1.00 3.995 312 CAGCTGTAGGAAGTGTGTGGTA 210 22 0 50.00 57.969 6.00 2.00 4.031 313 CTTGGCCACATTCTTAGGGAAC 138 22 0 50.00 62.039 6.00 0.00 4.039 314 TAGCTGGGTTTGTGGCTAGTCA 263 22 0 50.00 62.057 6.00 6.00 4.057 315 TGTAGGAAGTGTGTGGTACACCC 206 23 0 52.17 61.117 5.00 5.00 4.117 316 GTAGGAAGTGTGTGGTACACCCA 205 23 0 52.17 61.117 6.00 6.00 4.117 317 TAGGAAGTGTGTGGTACACCCAC 204 23 0 52.17 61.117 8.00 8.00 4.117 318 GCCCTGAAGAGCCTCATT 234 18 0 55.56 57.879 5.00 5.00 4.121 319 ACAGCTGTAGGAAGTGTGTGGTA 211 23 0 47.83 58.855 8.00 2.00 4.145 320 CAGCTGTAGGAAGTGTGTGGTAC 210 23 0 52.17 58.855 6.00 4.00 4.145 321 AGCTGTAGGAAGTGTGTGGTACA 209 23 0 47.83 58.855 4.00 3.00 4.145 322 GCTGTAGGAAGTGTGTGGTACA 208 22 0 50.00 57.817 4.00 3.00 4.183 323 TGGTACACCCACAATGTCTTAGC 193 23 0 47.83 61.184 5.00 2.00 4.184 324 ACACCCACAATGTCTTAGCTTT 189 22 0 40.91 57.804 5.00 3.00 4.196 325 ATCTTCAGTGTGTACATGGCGG 116 22 0 50.00 62.253 6.00 1.00 4.253 326 AGCTGGGTTTGTGGCTAGTCAT 262 22 0 50.00 62.275 6.00 4.00 4.275 327 GCTGTAGGAAGTGTGTGGTACAC 208 23 0 52.17 58.715 5.00 5.00 4.285 328 TTCTTAGGGAACATCTTCAGTGTG 128 24 0 41.67 59.690 6.00 2.00 4.310 329 ACAGCTGTAGGAAGTGTGTGGTAC 211 24 0 50.00 59.674 8.00 4.00 4.326 330 AGCTGTAGGAAGTGTGTGGTACAC 209 24 0 50.00 59.674 5.00 5.00 4.326 331 TTAGGGAAGGTCTTCAGTGCATT 163 23 0 43.48 61.327 6.00 4.00 4.327 332 CTGAAGAGCCTCATTTTCCCAC 231 22 0 50.00 62.336 5.00 0.00 4.336 333 GAAGAGCCTCATTTTCCCACAG 229 22 0 50.00 62.336 5.00 1.00 4.336 334 TTTGTGGCTAGTCATGGAGGTG 255 22 0 50.00 62.349 6.00 0.00 4.349 335 AGGGAACATCTTCAGTGTGTACA 123 23 0 43.48 58.639 6.00 6.00 4.361 336 GCAGTTACACCACCCCATTCTT 298 22 0 50.00 62.366 3.00 0.00 4.366 337 TATTAGGGAAGGTCTTCAGTGCAT 165 24 0 41.67 60.370 6.00 2.00 4.370 338 ACATCTTCAGTGTGTACATGGC 118 22 0 45.45 57.601 6.00 2.00 4.399 339 GGGAACATCTTCAGTGTGTACA 122 22 0 45.45 57.574 6.00 6.00 4.426 340 CTGTAGGAAGTGTGTGGTACACC 207 23 0 52.17 58.570 5.00 4.00 4.430 341 AGTGTGTGGTACACCCACAATGT 199 23 0 47.83 61.441 8.00 5.00 4.441 342 TGGTACACCCACAATGTCTTAG 193 22 0 45.45 57.543 5.00 3.00 4.457 343 TATTAGGGAAGGTCTTCAGTGC 165 22 0 45.45 57.520 6.00 2.00 4.480 344 TGGCCACATTCTTAGGGA 136 18 0 50.00 57.514 6.00 2.00 4.486 345 TGTAGGAAGTGTGTGGTACACC 206 22 0 50.00 57.509 5.00 4.00 4.491 346 GTGCATTCCCTTGGCCAC 147 18 0 61.11 62.504 6.00 4.00 4.504 347 GTGGTACACCCACAATGTCTTAG 194 23 0 47.83 58.433 5.00 3.00 4.567 348 TCAGTGTGTACATGGCGG 112 18 0 55.56 57.425 6.00 1.00 4.575 349 TCCCACAGCTGTAGGAAGTGTG 215 22 0 54.55 62.576 12.00 3.00 4.576 350 CACAGCTGTAGGAAGTGTGTGGT 212 23 0 52.17 61.580 8.00 0.00 4.580 351 GTGGTACACCCACAATGTCTTA 194 22 0 45.45 57.389 5.00 2.00 4.611 352 CCCACAATGTCTTAGCTTTTGTA 186 23 0 39.13 58.382 5.00 3.00 4.618 353 TCTTAGGGAACATCTTCAGTGTG 127 23 0 43.48 58.382 6.00 2.00 4.618 354 CAGTGCATTCCCTTGGCC 149 18 0 61.11 62.628 4.00 4.00 4.628 355 AGTGCATTCCCTTGGCCA 148 18 0 55.56 62.628 6.00 6.00 4.628 356 GTATTAGGGAAGGTCTTCAGTGC 166 23 0 47.83 58.358 6.00 2.00 4.642 357 GGTACACCCACAATGTCTTAGCTT 192 24 0 45.83 60.673 5.00 2.00 4.673 358 GGGAACATCTTCAGTGTGTACATG 122 24 0 45.83 60.700 6.00 5.00 4.700 359 GGAACATCTTCAGTGTGTACATGG 121 24 0 45.83 60.700 6.00 3.00 4.700 360 CACATTCTTAGGGAACATCTTCA 132 23 0 39.13 58.251 6.00 4.00 4.749 361 CCACATTCTTAGGGAACATCTT 133 22 0 40.91 57.236 4.00 4.00 4.764 362 AGGTCTTCAGTGCATTCCCTTG 156 22 0 50.00 62.786 4.00 2.00 4.786 363 TCTTAGGGAACATCTTCAGTGTGT 127 24 0 41.67 59.212 6.00 2.00 4.788 364 CATTCTTTCCGTGGCTCTCCTA 283 22 0 50.00 62.788 2.00 2.00 4.788 365 TGGCCACATTCTTAGGGAACAT 136 22 0 45.45 62.791 6.00 2.00 4.791 366 CACATTCTTAGGGAACATCTTCAG 132 24 0 41.67 59.196 6.00 2.00 4.804 367 CATTCTTAGGGAACATCTTCAGTG 130 24 0 41.67 59.196 6.00 3.00 4.804 368 CTCCTAGCTGGGTTTGTGGCTA 267 22 0 54.55 62.814 6.00 2.00 4.814 369 TCCTAGCTGGGTTTGTGGCTAG 266 22 0 54.55 62.814 6.00 4.00 4.814 370 TAGGGAAGGTCTTCAGTGCATTC 162 23 0 47.83 61.828 6.00 2.00 4.828 371 CAGTTACACCACCCCATTCTTTC 297 23 0 47.83 61.831 3.00 0.00 4.831 372 TACACCCACAATGTCTTAGCTTTT 190 24 0 37.50 59.162 5.00 1.00 4.838 373 ACCCACAATGTCTTAGCTTTTGTA 187 24 0 37.50 59.162 5.00 3.00 4.838 374 GAACATCTTCAGTGTGTACATGGC 120 24 0 45.83 60.841 6.00 2.00 4.841 375 GGGAAGGTCTTCAGTGCA 160 18 0 55.56 57.158 6.00 4.00 4.842 376 TCCTAGCTGGGTTTGTGG 266 18 0 55.56 57.142 6.00 0.00 4.858 377 TGTATTAGGGAAGGTCTTCAGTGC 167 24 0 45.83 60.860 6.00 2.00 4.860 378 GTATTAGGGAAGGTCTTCAGTGCA 166 24 0 45.83 60.860 6.00 4.00 4.860 379 AAGTGTGTGGTACACCCACAATG 200 23 0 47.83 61.918 8.00 6.00 4.918 380 GAGCCTCATTTTCCCACA 226 18 0 50.00 57.033 5.00 0.00 4.967 381 GTGTGGTACACCCACAATGTCTTA 196 24 0 45.83 60.969 6.00 2.00 4.969 382 CCCTTGGCCACATTCTTA 140 18 0 50.00 57.022 6.00 2.00 4.978 383 TTGGCCACATTCTTAGGG 137 18 0 50.00 57.022 6.00 0.00 4.978 384 GGGAACATCTTCAGTGTGTACAT 122 23 0 43.48 57.976 6.00 4.00 5.024 385 TTAGGGAACATCTTCAGTGTGTACA 125 25 0 40.00 59.975 6.00 6.00 5.025 386 AGGGAACATCTTCAGTGTGTACAT 123 24 0 41.67 58.969 6.00 4.00 5.031 387 CACATTCTTAGGGAACATCTTCAGT 132 25 0 40.00 59.947 6.00 4.00 5.053 388 ACATTCTTAGGGAACATCTTCAGTG 131 25 0 40.00 59.947 6.00 3.00 5.053 389 CATTCTTAGGGAACATCTTCAGTGT 130 25 0 40.00 59.947 6.00 4.00 5.053 390 ATTCTTAGGGAACATCTTCAGTGTG 129 25 0 40.00 59.947 6.00 2.00 5.053 391 TTTGTATTAGGGAAGGTCTTCAGTG 169 25 0 40.00 59.946 6.00 3.00 5.054 392 TGTGGTACACCCACAATGTCTTAG 195 24 0 45.83 61.086 6.00 3.00 5.086 393 TACACCCACAATGTCTTAGCTTT 190 23 0 39.13 57.892 5.00 3.00 5.108 394 GTACACCCACAATGTCTTAGCTTTT 191 25 0 40.00 59.890 5.00 1.00 5.110 395 CCCACAATGTCTTAGCTTTTGTATT 186 25 0 36.00 59.858 5.00 1.00 5.142 396 GCTTTTGTATTAGGGAAGGTCTTC 172 24 0 41.67 58.814 6.00 6.00 5.186 397 TAGCTGGGTTTGTGGCTAGTCAT 263 23 0 47.83 62.206 6.00 4.00 5.206 398 TTGTATTAGGGAAGGTCTTCAGTG 168 24 0 41.67 58.759 6.00 3.00 5.241 399 ACATCTTCAGTGTGTACATGGCG 118 23 0 47.83 62.286 6.00 2.00 5.286 400 GTACACCCACAATGTCTTAGCTTT 191 24 0 41.67 58.699 5.00 3.00 5.301 401 TAGGGAACATCTTCAGTGTGTACA 124 24 0 41.67 58.699 6.00 6.00 5.301 402 CCCACAATGTCTTAGCTTTTGTAT 186 24 0 37.50 58.698 5.00 2.00 5.302 403 GTGTGTGGTACACCCACAATGTC 198 23 0 52.17 62.364 8.00 3.00 5.364 404 AGCTTTTGTATTAGGGAAGGTCTTC 173 25 0 40.00 59.629 6.00 6.00 5.371 405 TTCTTAGGGAACATCTTCAGTGTGT 128 25 0 40.00 60.434 6.00 2.00 5.434 406 AGTTACACCACCCCATTCTTTCC 296 23 0 47.83 62.469 3.00 0.00 5.469 407 TGTGTGGTACACCCACAATGTCT 197 23 0 47.83 62.479 8.00 1.00 5.479 408 ATTAGGGAAGGTCTTCAGTGCATT 164 24 0 41.67 61.502 6.00 4.00 5.502 409 CACAGCTGTAGGAAGTGTGTGGTA 212 24 0 50.00 61.532 8.00 2.00 5.532 410 CAGCTGTAGGAAGTGTGTGGTACA 210 24 0 50.00 61.532 6.00 3.00 5.532 411 CCACATTCTTAGGGAACATCTTCA 133 24 0 41.67 61.537 6.00 4.00 5.537 412 TGTATTAGGGAAGGTCTTCAGTG 167 23 0 43.48 57.450 6.00 3.00 5.550 413 ACCCACAATGTCTTAGCTTTTGTAT 187 25 0 36.00 59.432 5.00 2.00 5.568 414 CTTAGGGAACATCTTCAGTGTGT 126 23 0 43.48 57.416 6.00 2.00 5.584 415 ACACCCACAATGTCTTAGCTTTTG 189 24 0 41.67 61.607 5.00 3.00 5.607 416 CACCCACAATGTCTTAGCTTTTGT 188 24 0 41.67 61.607 5.00 5.00 5.607 417 GTACACCCACAATGTCTTAGCTT 191 23 0 43.48 57.388 5.00 2.00 5.612 418 GGCCACATTCTTAGGGAACATCT 135 23 0 47.83 62.687 4.00 2.00 5.687 419 TCTTAGGGAACATCTTCAGTGTGTA 127 25 0 40.00 59.249 6.00 2.00 5.751 420 GCTTTTGTATTAGGGAAGGTCTT 172 23 0 39.13 57.202 4.00 4.00 5.798 421 CTAGCTGGGTTTGTGGCTAGTCA 264 23 0 52.17 62.841 6.00 6.00 5.841 422 GTAGGAAGTGTGTGGTACACCCAC 205 24 0 54.17 61.849 8.00 8.00 5.849 423 GGAACATCTTCAGTGTGTACATG 121 23 0 43.48 57.126 6.00 5.00 5.874 424 GAACATCTTCAGTGTGTACATGG 120 23 0 43.48 57.126 6.00 3.00 5.874 425 GTGGTACACCCACAATGTCTTAGC 194 24 0 50.00 61.894 5.00 2.00 5.894 426 AGCTTTTGTATTAGGGAAGGTCTT 173 24 0 37.50 58.100 4.00 4.00 5.900 427 TTTTGTATTAGGGAAGGTCTTCA 170 23 0 34.78 57.052 6.00 4.00 5.948 428 TTCTTAGGGAACATCTTCAGTGT 128 23 0 39.13 57.049 6.00 4.00 5.951 429 CTGTAGGAAGTGTGTGGTACACCC 207 24 0 54.17 61.959 5.00 5.00 5.959 430 TAGGGAACATCTTCAGTGTGTACAT 124 25 0 40.00 59.015 6.00 4.00 5.985 431 TGGTACACCCACAATGTCTTAGCT 193 24 0 45.83 62.000 5.00 4.00 6.000 432 CTTTTGTATTAGGGAAGGTCTTCA 171 24 0 37.50 57.993 6.00 4.00 6.007 433 TTTTGTATTAGGGAAGGTCTTCAG 170 24 0 37.50 57.993 6.00 2.00 6.007 434 TCTTAGGGAACATCTTCAGTGTGTAC 127 26 0 42.31 59.993 6.00 4.00 6.007 435 GTATTAGGGAAGGTCTTCAGTGCAT 166 25 0 44.00 61.054 6.00 2.00 6.054 436 GCTGTAGGAAGTGTGTGGTACACC 208 24 0 54.17 62.095 5.00 4.00 6.095 437 GCTTTTGTATTAGGGAAGGTCTTCA 172 25 0 40.00 61.112 6.00 4.00 6.112 438 CCCACAATGTCTTAGCTTTTGTATTA 186 26 0 34.62 59.872 5.00 3.00 6.128 439 CTTTTGTATTAGGGAAGGTCTTCAG 171 25 0 40.00 58.861 6.00 2.00 6.139 440 TTAGGGAACATCTTCAGTGTGTACAT 125 26 0 38.46 60.216 6.00 4.00 6.216 441 TTTTGTATTAGGGAAGGTCTTCAGT 170 25 0 36.00 58.743 6.00 4.00 6.257 442 GCCACATTCTTAGGGAACATCTTC 134 24 0 45.83 62.298 6.00 6.00 6.298 443 TCTTAGCTTTTGTATTAGGGAAGGTC 177 26 0 38.46 59.649 4.00 1.00 6.351 444 TAGCTTTTGTATTAGGGAAGGTCTTC 174 26 0 38.46 59.649 6.00 6.00 6.351 445 TGTCTTAGCTTTTGTATTAGGGAAGG 179 26 0 38.46 60.381 4.00 1.00 6.381 446 TTCTTAGGGAACATCTTCAGTGTGTA 128 26 0 38.46 60.429 6.00 2.00 6.429 447 CTTTTGTATTAGGGAAGGTCTTCAGT 171 26 0 38.46 59.556 6.00 4.00 6.444 448 TATTAGGGAAGGTCTTCAGTGCATT 165 25 0 40.00 61.463 6.00 4.00 6.463 449 TTTGTATTAGGGAAGGTCTTCAGT 169 24 0 37.50 57.525 6.00 4.00 6.475 450 CTTAGGGAACATCTTCAGTGTGTA 126 24 0 41.67 57.518 6.00 2.00 6.482 451 ACCCACAATGTCTTAGCTTTTGTATT 187 26 0 34.62 60.524 5.00 1.00 6.524 452 AGGGAACATCTTCAGTGTGTACATG 123 25 0 44.00 61.548 6.00 5.00 6.548 453 ACATTCTTAGGGAACATCTTCAGT 131 24 0 37.50 57.442 6.00 4.00 6.558 454 ATTCTTAGGGAACATCTTCAGTGT 129 24 0 37.50 57.442 6.00 4.00 6.558 455 TACACCCACAATGTCTTAGCTTTTG 190 25 0 40.00 61.563 5.00 3.00 6.563 456 CACCCACAATGTCTTAGCTTTTGTA 188 25 0 40.00 61.563 5.00 3.00 6.563 457 TCTTAGCTTTTGTATTAGGGAAGG 177 24 0 37.50 57.427 4.00 1.00 6.573 458 TTAGGGAACATCTTCAGTGTGTAC 125 24 0 41.67 57.376 6.00 4.00 6.624 459 AAGTGTGTGGTACACCCACAATGT 200 24 0 45.83 62.627 8.00 6.00 6.627 460 ACATTCTTAGGGAACATCTTCAGTGT 131 26 0 38.46 60.648 6.00 4.00 6.648 461 ATTCTTAGGGAACATCTTCAGTGTGT 129 26 0 38.46 60.648 6.00 2.00 6.648 462 CTTAGGGAACATCTTCAGTGTGTAC 126 25 0 44.00 58.344 6.00 4.00 6.656 463 TTAGCTTTTGTATTAGGGAAGGTC 175 24 0 37.50 57.300 4.00 1.00 6.700 464 TTAGCTTTTGTATTAGGGAAGGTCTT 175 26 0 34.62 59.264 4.00 4.00 6.736 465 GGTACACCCACAATGTCTTAGCTTT 192 25 0 44.00 61.766 5.00 3.00 6.766 466 CAATGTCTTAGCTTTTGTATTAGGGA 182 26 0 34.62 59.231 4.00 0.00 6.769 467 GTGTGGTACACCCACAATGTCTTAG 196 25 0 48.00 61.794 6.00 3.00 6.794 468 CTTAGGGAACATCTTCAGTGTGTACA 126 26 0 42.31 60.811 6.00 6.00 6.811 469 GTCTTAGCTTTTGTATTAGGGAAGG 178 25 0 40.00 58.167 4.00 1.00 6.833 470 TCTTAGCTTTTGTATTAGGGAAGGT 177 25 0 36.00 58.167 4.00 3.00 6.833 471 CTTAGCTTTTGTATTAGGGAAGGTC 176 25 0 40.00 58.167 4.00 1.00 6.833 472 TTAGCTTTTGTATTAGGGAAGGTCT 175 25 0 36.00 58.167 4.00 2.00 6.833 473 TAGCTTTTGTATTAGGGAAGGTCTT 174 25 0 36.00 58.167 4.00 4.00 6.833 474 TTAGGGAAGGTCTTCAGTGCATTC 163 24 0 45.83 62.910 6.00 2.00 6.910 475 TTGTATTAGGGAAGGTCTTCAGTGC 168 25 0 44.00 61.945 6.00 2.00 6.945 476 CTAGCTGGGTTTGTGGCTAGTCAT 264 24 0 50.00 62.946 6.00 4.00 6.946 477 CTTAGCTTTTGTATTAGGGAAGGTCTT 176 27 0 37.04 59.997 4.00 4.00 7.003 478 TTTTGTATTAGGGAAGGTCTTCAGTG 170 26 0 38.46 61.028 6.00 3.00 7.028 479 CTTAGCTTTTGTATTAGGGAAGGTCT 176 26 0 38.46 58.971 4.00 2.00 7.029 480 ACAATGTCTTAGCTTTTGTATTAGGGA 183 27 0 33.33 59.891 5.00 0.00 7.109 481 GTCTTAGCTTTTGTATTAGGGAAGGT 178 26 0 38.46 58.862 4.00 3.00 7.138 482 CACAGCTGTAGGAAGTGTGTGGTAC 212 25 0 52.00 62.233 8.00 4.00 7.233 483 ACAGCTGTAGGAAGTGTGTGGTACA 211 25 0 48.00 62.233 8.00 3.00 7.233 484 CAGCTGTAGGAAGTGTGTGGTACAC 210 25 0 52.00 62.233 6.00 5.00 7.233 485 ACACCCACAATGTCTTAGCTTTTGT 189 25 0 40.00 62.258 5.00 5.00 7.258 486 CAATGTCTTAGCTTTTGTATTAGGGAA 182 27 0 33.33 60.279 4.00 1.00 7.279 487 GTCTTAGCTTTTGTATTAGGGAAGGTC 178 27 0 40.74 60.283 4.00 1.00 7.283 488 CAATGTCTTAGCTTTTGTATTAGGG 182 25 0 36.00 57.709 4.00 0.00 7.291 489 CCACATTCTTAGGGAACATCTTCAG 133 25 0 44.00 62.299 6.00 2.00 7.299 490 TCTTAGCTTTTGTATTAGGGAAGGTCT 177 27 0 37.04 60.382 4.00 2.00 7.382 491 TAGGGAACATCTTCAGTGTGTACATG 124 26 0 42.31 61.505 6.00 5.00 7.505 492 TGTCTTAGCTTTTGTATTAGGGAAG 179 25 0 36.00 57.487 4.00 3.00 7.513 493 ACCCACAATGTCTTAGCTTTTGTATTA 187 27 0 33.33 60.517 5.00 3.00 7.517 494 ACAATGTCTTAGCTTTTGTATTAGGG 183 26 0 34.62 58.432 5.00 0.00 7.568 495 ATGTCTTAGCTTTTGTATTAGGGAAGG 180 27 0 37.04 60.573 4.00 1.00 7.573 496 CCCACAATGTCTTAGCTTTTGTATTAG 186 27 0 37.04 60.616 5.00 1.00 7.616 497 CCACAATGTCTTAGCTTTTGTATTAGG 185 27 0 37.04 60.616 5.00 0.00 7.616 498 CACAATGTCTTAGCTTTTGTATTAGGG 184 27 0 37.04 60.616 5.00 0.00 7.616 499 ATTCTTAGGGAACATCTTCAGTGTGTA 129 27 0 37.04 60.637 6.00 2.00 7.637 500 GTGGTACACCCACAATGTCTTAGCT 194 25 0 48.00 62.652 5.00 4.00 7.652 501 TTAGCTTTTGTATTAGGGAAGGTCTTC 175 27 0 37.04 60.660 6.00 6.00 7.660 502 CACCCACAATGTCTTAGCTTTTGTAT 188 26 0 38.46 61.720 5.00 2.00 7.720 503 AGCTTTTGTATTAGGGAAGGTCTTCA 173 26 0 38.46 61.823 6.00 4.00 7.823 504 GCTTTTGTATTAGGGAAGGTCTTCAG 172 26 0 42.31 61.823 6.00 2.00 7.823 505 AGCTGTAGGAAGTGTGTGGTACACC 209 25 0 52.00 62.860 5.00 4.00 7.860 506 AATGTCTTAGCTTTTGTATTAGGGAA 181 26 0 30.77 58.116 4.00 1.00 7.884 507 TGTCTTAGCTTTTGTATTAGGGAAGGT 179 27 0 37.04 60.995 4.00 3.00 7.995 508 CTTAGGGAACATCTTCAGTGTGTACAT 126 27 0 40.74 61.005 6.00 4.00 8.005 509 GTATTAGGGAAGGTCTTCAGTGCATT 166 26 0 42.31 62.082 6.00 4.00 8.082 510 AATGTCTTAGCTTTTGTATTAGGGAAG 181 27 0 33.33 58.903 4.00 3.00 8.097 511 TTCTTAGGGAACATCTTCAGTGTGTAC 128 27 0 40.74 61.101 6.00 4.00 8.101 512 ATGTCTTAGCTTTTGTATTAGGGAAG 180 26 0 34.62 57.811 4.00 3.00 8.189 513 GTACACCCACAATGTCTTAGCTTTTG 191 26 0 42.31 62.195 5.00 3.00 8.195 514 TACACCCACAATGTCTTAGCTTTTGT 190 26 0 38.46 62.195 5.00 5.00 8.195 515 ACACCCACAATGTCTTAGCTTTTGTA 189 26 0 38.46 62.195 5.00 3.00 8.195 516 CCACAATGTCTTAGCTTTTGTATTAG 185 26 0 34.62 57.766 5.00 1.00 8.234 517 CACAATGTCTTAGCTTTTGTATTAGG 184 26 0 34.62 57.766 5.00 0.00 8.234 518 CACATTCTTAGGGAACATCTTCAGTG 132 26 0 42.31 62.327 6.00 3.00 8.327 519 CATTCTTAGGGAACATCTTCAGTGTG 130 26 0 42.31 62.327 6.00 2.00 8.327 520 CTTTTGTATTAGGGAAGGTCTTCAGTG 171 27 0 40.74 61.738 6.00 3.00 8.738 521 GGTACACCCACAATGTCTTAGCTTTT 192 26 0 42.31 62.764 5.00 1.00 8.764 522 TAGCTTTTGTATTAGGGAAGGTCTTCA 174 27 0 37.04 61.778 6.00 4.00 8.778 523 ACAGCTGTAGGAAGTGTGTGGTACAC 211 26 0 50.00 62.885 8.00 5.00 8.885 524 CCACATTCTTAGGGAACATCTTCAGT 133 26 0 42.31 62.911 6.00 4.00 8.911 525 TATTAGGGAAGGTCTTCAGTGCATTC 165 26 0 42.31 62.924 6.00 2.00 8.924 526 TTTGTATTAGGGAAGGTCTTCAGTGC 169 26 0 42.31 62.934 6.00 2.00 8.934 527 TACACCCACAATGTCTTAGCTTTTGTA 190 27 0 37.04 62.136 5.00 3.00 9.136 528 ACACCCACAATGTCTTAGCTTTTGTAT 189 27 0 37.04 62.320 5.00 2.00 9.320 529 TCTTAGGGAACATCTTCAGTGTGTACA 127 27 0 40.74 62.355 6.00 6.00 9.355 530 GCTTTTGTATTAGGGAAGGTCTTCAGT 172 27 0 40.74 62.397 6.00 4.00 9.397 531 AGCTTTTGTATTAGGGAAGGTCTTCAG 173 27 0 40.74 62.483 6.00 2.00 9.483 532 TTAGGGAACATCTTCAGTGTGTACATG 125 27 0 40.74 62.561 6.00 5.00 9.561 533 CACCCACAATGTCTTAGCTTTTGTATT 188 27 0 37.04 62.689 5.00 1.00 9.689 534 GTACACCCACAATGTCTTAGCTTTTGT 191 27 0 40.74 62.787 5.00 5.00 9.787 535 CACATTCTTAGGGAACATCTTCAGTGT 132 27 0 40.74 62.934 6.00 4.00 9.934 536 ACATTCTTAGGGAACATCTTCAGTGTG 131 27 0 40.74 62.934 6.00 2.00 9.934 537 CATTCTTAGGGAACATCTTCAGTGTGT 130 27 0 40.74 62.934 6.00 2.00 9.934 primer3-2.3.6/test/primer_list_last/SP01-21.txt.for0000664000077300007730000005577012231156244020657 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGTGTCTTGTGGGTGTGTCTG 58 21 0 52.38 60.680 1.00 1.00 1.680 1 TGTCTTGTGGGTGTGTCTGTT 60 21 0 47.62 59.059 1.00 0.00 1.941 2 GTGTCTTGTGGGTGTGTCTGTT 59 22 0 50.00 59.972 1.00 0.00 2.028 3 GAACATGTGTGGCATTGTGT 0 20 0 45.00 57.775 6.00 0.00 2.225 4 GTGTCTTGTGGGTGTGTCTGT 59 21 0 52.38 58.472 1.00 0.00 2.528 5 TGTCTTGTGGGTGTGTCTGT 60 20 0 50.00 57.431 1.00 0.00 2.569 6 GTGTCTTGTGGGTGTGTCTG 59 20 0 55.00 57.431 1.00 1.00 2.569 7 TGTGTCTTGTGGGTGTGTCT 58 20 0 50.00 57.431 1.00 0.00 2.569 8 TGTCTTGTGGGTGTGTCTGTTA 60 22 0 45.45 59.108 2.00 2.00 2.892 9 TTGTGGGTGTGTCTGTTAAACA 64 22 0 40.91 58.997 7.00 7.00 3.003 10 GTGTCTTGTGGGTGTGTCTGTTA 59 23 0 47.83 59.984 2.00 2.00 3.016 11 CTTGTGGGTGTGTCTGTTAAACA 63 23 0 43.48 59.981 7.00 7.00 3.019 12 GAACATGTGTGGCATTGTGTA 0 21 0 42.86 57.880 6.00 2.00 3.120 13 TGTCTTGTGGGTGTGTCTGTTAA 60 23 0 43.48 60.493 4.00 4.00 3.493 14 TGTGGGTGTGTCTGTTAAACA 65 21 0 42.86 57.489 7.00 7.00 3.511 15 TGTGTCTTGTGGGTGTGTCTGT 58 22 0 50.00 61.549 1.00 0.00 3.549 16 AAACATGTGTGGTATTGTGCA 81 21 0 38.10 57.391 6.00 4.00 3.609 17 TTGTGGGTGTGTCTGTTAAACAT 64 23 0 39.13 59.319 7.00 5.00 3.681 18 GAACATGTGTGGCATTGTGTAT 0 22 0 40.91 58.283 6.00 2.00 3.717 19 TGGGTGTGTCTGTTAAACATGTG 67 23 0 43.48 60.746 7.00 4.00 3.746 20 TGTGGGTGTGTCTGTTAAACATG 65 23 0 43.48 60.746 7.00 5.00 3.746 21 TCTTGTGGGTGTGTCTGTTAAA 62 22 0 40.91 58.187 4.00 3.00 3.813 22 AGCTTCTTGACTGTGTGTTGTG 128 22 0 45.45 58.075 4.00 0.00 3.925 23 TCTTGTGGGTGTGTCTGTTAAAC 62 23 0 43.48 59.063 5.00 5.00 3.937 24 GTCTTGTGGGTGTGTCTGTTAAA 61 23 0 43.48 59.063 4.00 3.00 3.937 25 TGGATGTAGCTTCTTGACTGTG 121 22 0 45.45 58.036 4.00 1.00 3.964 26 AGCTTCTTGACTGTGTGTTGTGT 128 23 0 43.48 58.981 4.00 0.00 4.019 27 GGATGTAGCTTCTTGACTGTGTG 122 23 0 47.83 58.925 4.00 0.00 4.075 28 TGGATGTAGCTTCTTGACTGTGT 121 23 0 43.48 58.925 4.00 1.00 4.075 29 TTAAACATGTGTGGTATTGTGCA 79 23 0 34.78 58.925 6.00 4.00 4.075 30 GGGTGTGTCTGTTAAACATGTG 68 22 0 45.45 57.912 7.00 4.00 4.088 31 TGGGTGTGTCTGTTAAACATGT 67 22 0 40.91 57.912 7.00 6.00 4.088 32 GTGGGTGTGTCTGTTAAACATG 66 22 0 45.45 57.912 7.00 5.00 4.088 33 TGTGGGTGTGTCTGTTAAACAT 65 22 0 40.91 57.912 7.00 5.00 4.088 34 AACATGTGTGGCATTGTGTATACA 1 24 0 37.50 60.103 8.00 8.00 4.103 35 GTCTTGTGGGTGTGTCTGTTAAAC 61 24 0 45.83 59.875 5.00 5.00 4.125 36 CATGTGTGGCATTGTGTATACA 3 22 0 40.91 57.850 8.00 8.00 4.150 37 AAACATGTGTGGTATTGTGCAT 81 22 0 36.36 57.811 6.00 2.00 4.189 38 GGGTGTGTCTGTTAAACATGTGT 68 23 0 43.48 58.808 7.00 6.00 4.192 39 GTGGGTGTGTCTGTTAAACATGT 66 23 0 43.48 58.808 7.00 6.00 4.192 40 ACATGTGTGGCATTGTGTATACA 2 23 0 39.13 58.766 8.00 8.00 4.234 41 CTTGTGGGTGTGTCTGTTAAACAT 63 24 0 41.67 60.239 7.00 5.00 4.239 42 GGATGTAGCTTCTTGACTGTGTGT 122 24 0 45.83 59.748 4.00 0.00 4.252 43 GTTAAACATGTGTGGTATTGTGCA 78 24 0 37.50 59.733 6.00 4.00 4.267 44 TGTTAAACATGTGTGGTATTGTGC 77 24 0 37.50 59.733 7.00 2.00 4.267 45 GTCTTGTGGGTGTGTCTGTTAA 61 22 0 45.45 57.635 4.00 4.00 4.365 46 TAAACATGTGTGGTATTGTGCA 80 22 0 36.36 57.504 6.00 4.00 4.496 47 TGTGTGTTGTGTATGGATGTAGC 108 23 0 43.48 58.499 2.00 2.00 4.501 48 TGTGTGTTGTGTATGGATGTAGCT 108 24 0 41.67 59.493 4.00 4.00 4.507 49 ATGGATGTAGCTTCTTGACTGTG 120 23 0 43.48 58.408 4.00 1.00 4.592 50 GAACATGTGTGGCATTGTGTATA 0 23 0 39.13 58.360 6.00 4.00 4.640 51 TTGTGCATATACATGTGTGTTGTG 95 24 0 37.50 59.345 6.00 2.00 4.655 52 ATGGATGTAGCTTCTTGACTGTGT 120 24 0 41.67 59.238 4.00 1.00 4.762 53 TTAAACATGTGTGGTATTGTGCAT 79 24 0 33.33 59.233 6.00 2.00 4.767 54 GAACATGTGTGGCATTGTGTATAC 0 24 0 41.67 59.208 6.00 6.00 4.792 55 CTTGTGGGTGTGTCTGTTAAAC 63 22 0 45.45 57.176 5.00 5.00 4.824 56 TAGCTTCTTGACTGTGTGTTGTG 127 23 0 43.48 58.160 4.00 0.00 4.840 57 TGTAGCTTCTTGACTGTGTGTTG 125 23 0 43.48 58.160 4.00 0.00 4.840 58 TGTGTTGTGTATGGATGTAGCTTC 110 24 0 41.67 59.091 4.00 4.00 4.909 59 TAGCTTCTTGACTGTGTGTTGTGT 127 24 0 41.67 59.030 4.00 0.00 4.970 60 GTAGCTTCTTGACTGTGTGTTGTG 126 24 0 45.83 59.030 4.00 0.00 4.970 61 TGTAGCTTCTTGACTGTGTGTTGT 125 24 0 41.67 59.030 4.00 0.00 4.970 62 TGTGTCTGTTAAACATGTGTGG 71 22 0 40.91 57.024 7.00 2.00 4.976 63 TGTGTTGTGTATGGATGTAGCTTCT 110 25 0 40.00 60.002 4.00 2.00 5.002 64 GTTAAACATGTGTGGTATTGTGCAT 78 25 0 36.00 59.991 6.00 2.00 5.009 65 TGTGTCTGTTAAACATGTGTGGT 71 23 0 39.13 57.983 7.00 0.00 5.017 66 GTGTGTCTGTTAAACATGTGTGG 70 23 0 43.48 57.983 7.00 2.00 5.017 67 GGTGTGTCTGTTAAACATGTGTG 69 23 0 43.48 57.983 7.00 4.00 5.017 68 TGTGCATATACATGTGTGTTGTG 96 23 0 39.13 57.923 6.00 2.00 5.077 69 AAACATGTGTGGTATTGTGCATA 81 23 0 34.78 57.903 6.00 4.00 5.097 70 TAAACATGTGTGGTATTGTGCAT 80 23 0 34.78 57.903 6.00 2.00 5.097 71 GTGTGTTGTGTATGGATGTAGCTTC 109 25 0 44.00 59.880 4.00 4.00 5.120 72 GTGTGTCTGTTAAACATGTGTGGT 70 24 0 41.67 58.868 7.00 0.00 5.132 73 TTGTGCATATACATGTGTGTTGTGT 95 25 0 36.00 60.151 6.00 0.00 5.151 74 ATGTGTGTTGTGTATGGATGTAGC 107 24 0 41.67 58.842 2.00 2.00 5.158 75 GTAGCTTCTTGACTGTGTGTTGTGT 126 25 0 44.00 59.835 4.00 0.00 5.165 76 TTGTGTATGGATGTAGCTTCTTGA 114 24 0 37.50 58.834 4.00 2.00 5.166 77 TGTGCATATACATGTGTGTTGTGT 96 24 0 37.50 58.828 6.00 0.00 5.172 78 ATGTGTGTTGTGTATGGATGTAGCT 107 25 0 40.00 59.772 4.00 4.00 5.228 79 GTGTCTTGTGGGTGTGTCTGTTAA 59 24 0 45.83 61.270 4.00 4.00 5.270 80 GATGTAGCTTCTTGACTGTGTGTTG 123 25 0 44.00 60.272 4.00 0.00 5.272 81 ACATGTGTGTTGTGTATGGATGT 105 23 0 39.13 57.646 6.00 0.00 5.354 82 ATTGTGCATATACATGTGTGTTGTG 94 25 0 36.00 59.637 6.00 2.00 5.363 83 TTGTGTATGGATGTAGCTTCTTGAC 114 25 0 40.00 59.615 4.00 1.00 5.385 84 GTTGTGTATGGATGTAGCTTCTTGA 113 25 0 40.00 59.615 4.00 2.00 5.385 85 TGTTGTGTATGGATGTAGCTTCTTG 112 25 0 40.00 60.458 4.00 2.00 5.458 86 ATGTAGCTTCTTGACTGTGTGTTG 124 24 0 41.67 58.520 4.00 0.00 5.480 87 GGTGTGTCTGTTAAACATGTGTGG 69 24 0 45.83 61.509 7.00 2.00 5.509 88 GGGTGTGTCTGTTAAACATGTGTG 68 24 0 45.83 61.509 7.00 4.00 5.509 89 TGGGTGTGTCTGTTAAACATGTGT 67 24 0 41.67 61.509 7.00 6.00 5.509 90 GTGGGTGTGTCTGTTAAACATGTG 66 24 0 45.83 61.509 7.00 4.00 5.509 91 TGTGGGTGTGTCTGTTAAACATGT 65 24 0 41.67 61.509 7.00 6.00 5.509 92 TATGGATGTAGCTTCTTGACTGTG 119 24 0 41.67 58.476 4.00 1.00 5.524 93 TGTATGGATGTAGCTTCTTGACTG 117 24 0 41.67 58.476 4.00 1.00 5.524 94 TGTGTATGGATGTAGCTTCTTGA 115 23 0 39.13 57.467 4.00 2.00 5.533 95 CTGTTAAACATGTGTGGTATTGTGC 76 25 0 40.00 60.599 7.00 2.00 5.599 96 TGGATGTAGCTTCTTGACTGTGTG 121 24 0 45.83 61.619 4.00 0.00 5.619 97 AACATGTGTGGCATTGTGTATAC 1 23 0 39.13 57.373 6.00 6.00 5.627 98 TGTGTATGGATGTAGCTTCTTGAC 115 24 0 41.67 58.339 4.00 1.00 5.661 99 ATGTAGCTTCTTGACTGTGTGTTGT 124 25 0 40.00 59.333 4.00 0.00 5.667 100 TGGTATTGTGCATATACATGTGTG 90 24 0 37.50 58.315 6.00 4.00 5.685 101 TGTGGTATTGTGCATATACATGTG 88 24 0 37.50 58.315 6.00 4.00 5.685 102 TGTGTGGTATTGTGCATATACATG 86 24 0 37.50 58.315 6.00 6.00 5.685 103 CATGTGTGGTATTGTGCATATACA 84 24 0 37.50 58.315 6.00 6.00 5.685 104 TATGGATGTAGCTTCTTGACTGTGT 119 25 0 40.00 59.274 4.00 1.00 5.726 105 GTATGGATGTAGCTTCTTGACTGTG 118 25 0 44.00 59.274 4.00 1.00 5.726 106 TGTATGGATGTAGCTTCTTGACTGT 117 25 0 40.00 59.274 4.00 3.00 5.726 107 GTGTATGGATGTAGCTTCTTGACTG 116 25 0 44.00 59.274 4.00 1.00 5.726 108 TGTGTATGGATGTAGCTTCTTGACT 115 25 0 40.00 59.274 4.00 1.00 5.726 109 AGCTTCTTGACTGTGTGTTGTGTG 128 24 0 45.83 61.730 4.00 0.00 5.730 110 TTAAACATGTGTGGTATTGTGCATA 79 25 0 32.00 59.268 6.00 4.00 5.732 111 TCTTGTGGGTGTGTCTGTTAAACA 62 24 0 41.67 61.733 7.00 7.00 5.733 112 TGTCTTGTGGGTGTGTCTGTTAAA 60 24 0 41.67 61.733 4.00 3.00 5.733 113 AAACATGTGTGGTATTGTGCATAT 81 24 0 33.33 58.262 6.00 4.00 5.738 114 TGTGTGTTGTGTATGGATGTAGCTT 108 25 0 40.00 60.742 4.00 2.00 5.742 115 TGTGTTGTGTATGGATGTAGCTT 110 23 0 39.13 57.257 4.00 2.00 5.743 116 TGTGTCTTGTGGGTGTGTCTGTT 58 23 0 47.83 62.831 1.00 0.00 5.831 117 GTGTGTTGTGTATGGATGTAGCTT 109 24 0 41.67 58.137 4.00 2.00 5.863 118 GCATATACATGTGTGTTGTGTATGG 99 25 0 40.00 59.136 6.00 3.00 5.864 119 TGGTATTGTGCATATACATGTGTGT 90 25 0 36.00 59.136 6.00 6.00 5.864 120 GTGGTATTGTGCATATACATGTGTG 89 25 0 40.00 59.136 6.00 4.00 5.864 121 TGTGGTATTGTGCATATACATGTGT 88 25 0 36.00 59.136 6.00 6.00 5.864 122 GTGTGGTATTGTGCATATACATGTG 87 25 0 40.00 59.136 6.00 4.00 5.864 123 TGTGTGGTATTGTGCATATACATGT 86 25 0 36.00 59.136 6.00 6.00 5.864 124 ACATGTGTGGTATTGTGCATATACA 83 25 0 36.00 59.136 6.00 6.00 5.864 125 TGTGTCTGTTAAACATGTGTGGTA 71 24 0 37.50 58.069 7.00 2.00 5.931 126 GTTAAACATGTGTGGTATTGTGC 78 23 0 39.13 57.060 6.00 2.00 5.940 127 GGATGTAGCTTCTTGACTGTGTGTT 122 25 0 44.00 60.962 4.00 0.00 5.962 128 TTGTGTATGGATGTAGCTTCTTG 114 23 0 39.13 57.036 4.00 2.00 5.964 129 TTGTGGGTGTGTCTGTTAAACATG 64 24 0 41.67 61.967 7.00 5.00 5.967 130 GTTAAACATGTGTGGTATTGTGCATA 78 26 0 34.62 60.001 6.00 4.00 6.001 131 TAAACATGTGTGGTATTGTGCATA 80 24 0 33.33 57.988 6.00 4.00 6.012 132 GTATGGATGTAGCTTCTTGACTGTGT 118 26 0 42.31 60.018 4.00 1.00 6.018 133 GTGTATGGATGTAGCTTCTTGACTGT 116 26 0 42.31 60.018 4.00 3.00 6.018 134 GTGTGTCTGTTAAACATGTGTGGTA 70 25 0 40.00 58.919 7.00 2.00 6.081 135 TCTGTTAAACATGTGTGGTATTGTG 75 25 0 36.00 58.912 7.00 0.00 6.088 136 TGTCTGTTAAACATGTGTGGTATTG 73 25 0 36.00 58.912 7.00 0.00 6.088 137 GTTGTGTATGGATGTAGCTTCTTG 113 24 0 41.67 57.904 4.00 2.00 6.096 138 TGTTGTGTATGGATGTAGCTTCTT 112 24 0 37.50 57.904 4.00 4.00 6.096 139 GTGGTATTGTGCATATACATGTGTGT 89 26 0 38.46 59.900 6.00 6.00 6.100 140 GTGTGGTATTGTGCATATACATGTGT 87 26 0 38.46 59.900 6.00 6.00 6.100 141 CATGTGTGTTGTGTATGGATGTAG 106 24 0 41.67 57.896 4.00 0.00 6.104 142 TGTGCATATACATGTGTGTTGTGTA 96 25 0 36.00 58.882 6.00 2.00 6.118 143 TTGTGCATATACATGTGTGTTGTGTA 95 26 0 34.62 60.156 6.00 2.00 6.156 144 ACATGTGTGTTGTGTATGGATGTAG 105 25 0 40.00 58.761 6.00 0.00 6.239 145 ACATGTGTGTTGTGTATGGATGTA 105 24 0 37.50 57.747 6.00 2.00 6.253 146 TACATGTGTGTTGTGTATGGATGT 104 24 0 37.50 57.747 6.00 0.00 6.253 147 GTGTTGTGTATGGATGTAGCTTCTT 111 25 0 40.00 58.712 4.00 4.00 6.288 148 GTCTGTTAAACATGTGTGGTATTGTG 74 26 0 38.46 59.680 7.00 0.00 6.320 149 TGTCTGTTAAACATGTGTGGTATTGT 73 26 0 34.62 59.680 7.00 0.00 6.320 150 GTGTCTGTTAAACATGTGTGGTATTG 72 26 0 38.46 59.680 7.00 0.00 6.320 151 TGTGTCTGTTAAACATGTGTGGTATT 71 26 0 34.62 59.680 7.00 1.00 6.320 152 TATTGTGCATATACATGTGTGTTGTG 93 26 0 34.62 59.659 6.00 2.00 6.341 153 GTTGTGTATGGATGTAGCTTCTTGAC 113 26 0 42.31 60.343 4.00 1.00 6.343 154 GGTATTGTGCATATACATGTGTGTTG 91 26 0 38.46 60.346 6.00 4.00 6.346 155 TGGTATTGTGCATATACATGTGTGTT 90 26 0 34.62 60.346 6.00 4.00 6.346 156 AACATGTGTGGTATTGTGCATATACA 82 26 0 34.62 60.346 6.00 6.00 6.346 157 ATGTGTGGTATTGTGCATATACATG 85 25 0 36.00 58.650 6.00 6.00 6.350 158 CATGTGTGGTATTGTGCATATACAT 84 25 0 36.00 58.650 6.00 4.00 6.350 159 GATGTAGCTTCTTGACTGTGTGTT 123 24 0 41.67 57.626 4.00 0.00 6.374 160 ATTGTGCATATACATGTGTGTTGTGT 94 26 0 34.62 60.393 6.00 0.00 6.393 161 TTGTGTATGGATGTAGCTTCTTGACT 114 26 0 38.46 60.455 4.00 1.00 6.455 162 TTAAACATGTGTGGTATTGTGCATAT 79 26 0 30.77 59.536 6.00 4.00 6.464 163 CATGTGTGTTGTGTATGGATGTAGC 106 25 0 44.00 61.472 4.00 2.00 6.472 164 TGTGTCTGTTAAACATGTGTGGTAT 71 25 0 36.00 58.424 7.00 2.00 6.576 165 ATGTGTGGTATTGTGCATATACATGT 85 26 0 34.62 59.420 6.00 6.00 6.580 166 ACATGTGTGGTATTGTGCATATACAT 83 26 0 34.62 59.420 6.00 4.00 6.580 167 GTGTTGTGTATGGATGTAGCTTCT 111 24 0 41.67 57.400 4.00 2.00 6.600 168 TGCATATACATGTGTGTTGTGTATG 98 25 0 36.00 58.378 6.00 5.00 6.622 169 AAACATGTGTGGTATTGTGCATATA 81 25 0 32.00 58.331 6.00 4.00 6.669 170 TAAACATGTGTGGTATTGTGCATAT 80 25 0 32.00 58.331 6.00 4.00 6.669 171 TAGCTTCTTGACTGTGTGTTGTGTG 127 25 0 44.00 61.678 4.00 0.00 6.678 172 TGTAGCTTCTTGACTGTGTGTTGTG 125 25 0 44.00 61.678 4.00 0.00 6.678 173 GTGTGTTGTGTATGGATGTAGCTTCT 109 26 0 42.31 60.727 4.00 2.00 6.727 174 TGTGTCTTGTGGGTGTGTCTGTTA 58 24 0 45.83 62.734 2.00 2.00 6.734 175 ATACATGTGTGTTGTGTATGGATG 103 24 0 37.50 57.241 6.00 0.00 6.759 176 GTGTGTCTGTTAAACATGTGTGGTAT 70 26 0 38.46 59.220 7.00 2.00 6.780 177 ATGGATGTAGCTTCTTGACTGTGTG 120 25 0 44.00 61.791 4.00 0.00 6.791 178 GAACATGTGTGGCATTGTGTATACA 0 25 0 40.00 61.810 8.00 8.00 6.810 179 GTGCATATACATGTGTGTTGTGTATG 97 26 0 38.46 59.190 6.00 5.00 6.810 180 TGTGCATATACATGTGTGTTGTGTAT 96 26 0 34.62 59.190 6.00 3.00 6.810 181 GCATATACATGTGTGTTGTGTATGGA 99 26 0 38.46 60.811 6.00 1.00 6.811 182 CTGTTAAACATGTGTGGTATTGTG 76 24 0 37.50 57.140 7.00 0.00 6.860 183 ATACATGTGTGTTGTGTATGGATGT 103 25 0 36.00 58.124 6.00 0.00 6.876 184 TCTTGTGGGTGTGTCTGTTAAACAT 62 25 0 40.00 61.898 7.00 5.00 6.898 185 AAACATGTGTGGTATTGTGCATATAC 81 26 0 34.62 59.095 6.00 6.00 6.905 186 ATGTGTGTTGTGTATGGATGTAGCTT 107 26 0 38.46 60.948 4.00 2.00 6.948 187 AACATGTGTGGTATTGTGCATATA 82 24 0 33.33 57.002 6.00 4.00 6.998 188 GATGTAGCTTCTTGACTGTGTGTTGT 123 26 0 42.31 61.001 4.00 0.00 7.001 189 CATGTGTGGTATTGTGCATATACATG 84 26 0 38.46 61.147 6.00 6.00 7.147 190 GGTATTGTGCATATACATGTGTGTT 91 25 0 36.00 57.851 6.00 4.00 7.149 191 AACATGTGTGGTATTGTGCATATAC 82 25 0 36.00 57.851 6.00 6.00 7.149 192 GTGTTGTGTATGGATGTAGCTTCTTG 111 26 0 42.31 61.157 4.00 2.00 7.157 193 TGTGTTGTGTATGGATGTAGCTTCTT 110 26 0 38.46 61.157 4.00 4.00 7.157 194 TACATGTGTGTTGTGTATGGATGTA 104 25 0 36.00 57.841 6.00 2.00 7.159 195 TACATGTGTGTTGTGTATGGATGTAG 104 26 0 38.46 58.815 6.00 0.00 7.185 196 GGTGTGTCTGTTAAACATGTGTGGT 69 25 0 44.00 62.217 7.00 0.00 7.217 197 GTGGGTGTGTCTGTTAAACATGTGT 66 25 0 44.00 62.217 7.00 6.00 7.217 198 GTTAAACATGTGTGGTATTGTGCATAT 78 27 0 33.33 60.227 6.00 4.00 7.227 199 CATATACATGTGTGTTGTGTATGGATG 100 27 0 37.04 60.230 6.00 0.00 7.230 200 TGTTAAACATGTGTGGTATTGTGCA 77 25 0 36.00 62.264 7.00 4.00 7.264 201 TGGATGTAGCTTCTTGACTGTGTGT 121 25 0 44.00 62.322 4.00 0.00 7.322 202 TTGTGCATATACATGTGTGTTGTGTAT 95 27 0 33.33 60.389 6.00 3.00 7.389 203 ATTGTGCATATACATGTGTGTTGTGTA 94 27 0 33.33 60.389 6.00 2.00 7.389 204 TATTGTGCATATACATGTGTGTTGTGT 93 27 0 33.33 60.389 6.00 0.00 7.389 205 GTATTGTGCATATACATGTGTGTTGTG 92 27 0 37.04 60.389 6.00 2.00 7.389 206 GTGTCTGTTAAACATGTGTGGTATTGT 72 27 0 37.04 60.396 7.00 0.00 7.396 207 GTGTGTCTGTTAAACATGTGTGGTATT 70 27 0 37.04 60.396 7.00 1.00 7.396 208 TGTTGTGTATAAATGTTGTGTCTTCA 31 26 0 30.77 58.596 4.00 1.00 7.404 209 GTCTTGTGGGTGTGTCTGTTAAACA 61 25 0 44.00 62.427 7.00 7.00 7.427 210 TGTCTTGTGGGTGTGTCTGTTAAAC 60 25 0 44.00 62.427 5.00 5.00 7.427 211 GTGTCTTGTGGGTGTGTCTGTTAAA 59 25 0 44.00 62.427 4.00 3.00 7.427 212 TTAAACATGTGTGGTATTGTGCATATA 79 27 0 29.63 59.560 6.00 4.00 7.440 213 AGCTTCTTGACTGTGTGTTGTGTGT 128 25 0 44.00 62.443 4.00 0.00 7.443 214 AACATGTGTGGTATTGTGCATATACAT 82 27 0 33.33 60.562 6.00 4.00 7.562 215 TAAACATGTGTGGTATTGTGCATATA 80 26 0 30.77 58.395 6.00 4.00 7.605 216 GTGTTGTGTATAAATGTTGTGTCTTCA 30 27 0 33.33 59.358 4.00 1.00 7.642 217 TGTGTTGTGTATAAATGTTGTGTCTTC 29 27 0 33.33 59.358 4.00 0.00 7.642 218 TTGTGGGTGTGTCTGTTAAACATGT 64 25 0 40.00 62.648 7.00 6.00 7.648 219 TATACATGTGTGTTGTGTATGGATG 102 25 0 36.00 57.352 6.00 0.00 7.648 220 CATATACATGTGTGTTGTGTATGGA 100 25 0 36.00 57.352 6.00 0.00 7.648 221 TGCATATACATGTGTGTTGTGTATGG 98 26 0 38.46 61.654 6.00 3.00 7.654 222 TGTGGTATTGTGCATATACATGTGTG 88 26 0 38.46 61.654 6.00 4.00 7.654 223 TGTGTGGTATTGTGCATATACATGTG 86 26 0 38.46 61.654 6.00 4.00 7.654 224 TATGGATGTAGCTTCTTGACTGTGTG 119 26 0 42.31 61.740 4.00 0.00 7.740 225 TGTATGGATGTAGCTTCTTGACTGTG 117 26 0 42.31 61.740 4.00 1.00 7.740 226 TGTGTATGGATGTAGCTTCTTGACTG 115 26 0 42.31 61.740 4.00 1.00 7.740 227 CTTGTGGGTGTGTCTGTTAAACATG 63 25 0 44.00 62.749 7.00 5.00 7.749 228 ATACATGTGTGTTGTGTATGGATGTA 103 26 0 34.62 58.200 6.00 2.00 7.800 229 TATACATGTGTGTTGTGTATGGATGT 102 26 0 34.62 58.200 6.00 0.00 7.800 230 ATGTAGCTTCTTGACTGTGTGTTGTG 124 26 0 42.31 61.844 4.00 0.00 7.844 231 GTCTGTTAAACATGTGTGGTATTGT 74 25 0 36.00 57.143 7.00 0.00 7.857 232 GTGTCTGTTAAACATGTGTGGTATT 72 25 0 36.00 57.143 7.00 1.00 7.857 233 TAAACATGTGTGGTATTGTGCATATAC 80 27 0 33.33 59.133 6.00 6.00 7.867 234 ATACATGTGTGTTGTGTATGGATGTAG 103 27 0 37.04 59.113 6.00 0.00 7.887 235 TATTGTGCATATACATGTGTGTTGT 93 25 0 32.00 57.073 6.00 2.00 7.927 236 GTATTGTGCATATACATGTGTGTTG 92 25 0 36.00 57.073 6.00 4.00 7.927 237 GCATATACATGTGTGTTGTGTATGGAT 99 27 0 37.04 61.010 6.00 2.00 8.010 238 GGTATTGTGCATATACATGTGTGTTGT 91 27 0 37.04 61.034 6.00 2.00 8.034 239 GTGGTATTGTGCATATACATGTGTGTT 89 27 0 37.04 61.034 6.00 4.00 8.034 240 TGTTGTGTATGGATGTAGCTTCTTGA 112 26 0 38.46 62.056 4.00 2.00 8.056 241 GTATTGTGCATATACATGTGTGTTGT 92 26 0 34.62 57.913 6.00 2.00 8.087 242 GTTGTGTATGGATGTAGCTTCTTGACT 113 27 0 40.74 61.126 4.00 1.00 8.126 243 GGTGTGTCTGTTAAACATGTGTGGTA 69 26 0 42.31 62.150 7.00 2.00 8.150 244 ACATGTGTGTTGTGTATGGATGTAGC 105 26 0 42.31 62.163 6.00 2.00 8.163 245 TCTGTTAAACATGTGTGGTATTGTGC 75 26 0 38.46 62.185 7.00 2.00 8.185 246 CATGTGTGTTGTGTATGGATGTAGCT 106 26 0 42.31 62.266 4.00 4.00 8.266 247 ATATACATGTGTGTTGTGTATGGATG 101 26 0 34.62 57.730 6.00 0.00 8.270 248 CATATACATGTGTGTTGTGTATGGAT 100 26 0 34.62 57.730 6.00 2.00 8.270 249 TGTGTTGTGTATAAATGTTGTGTCTT 29 26 0 30.77 57.700 4.00 0.00 8.300 250 TAGCTTCTTGACTGTGTGTTGTGTGT 127 26 0 42.31 62.367 4.00 0.00 8.367 251 GTAGCTTCTTGACTGTGTGTTGTGTG 126 26 0 46.15 62.367 4.00 0.00 8.367 252 TGTAGCTTCTTGACTGTGTGTTGTGT 125 26 0 42.31 62.367 4.00 0.00 8.367 253 TGTGTGTTGTGTATGGATGTAGCTTC 108 26 0 42.31 62.368 4.00 4.00 8.368 254 TGTTAAACATGTGTGGTATTGTGCAT 77 26 0 34.62 62.397 7.00 2.00 8.397 255 AAACATGTGTGGTATTGTGCATATACA 81 27 0 33.33 61.445 6.00 6.00 8.445 256 ATGGATGTAGCTTCTTGACTGTGTGT 120 26 0 42.31 62.454 4.00 0.00 8.454 257 ATATACATGTGTGTTGTGTATGGATGT 101 27 0 33.33 58.526 6.00 0.00 8.474 258 GTCTTGTGGGTGTGTCTGTTAAACAT 61 26 0 42.31 62.553 7.00 5.00 8.553 259 TGTGCATATACATGTGTGTTGTGTATG 96 27 0 37.04 61.688 6.00 5.00 8.688 260 TATACATGTGTGTTGTGTATGGATGTA 102 27 0 33.33 58.271 6.00 2.00 8.729 261 GTGTGTTGTGTATGGATGTAGCTTCTT 109 27 0 40.74 61.811 4.00 4.00 8.811 262 ATGTGTGGTATTGTGCATATACATGTG 85 27 0 37.04 61.814 6.00 4.00 8.814 263 CATGTGTGGTATTGTGCATATACATGT 84 27 0 37.04 61.814 6.00 6.00 8.814 264 ACATGTGTGGTATTGTGCATATACATG 83 27 0 37.04 61.814 6.00 6.00 8.814 265 ATGTGTTGTGTATAAATGTTGTGTCTT 28 27 0 29.63 58.041 4.00 0.00 8.959 266 TGTCTGTTAAACATGTGTGGTATTGTG 73 27 0 37.04 62.089 7.00 0.00 9.089 267 TGTGTCTGTTAAACATGTGTGGTATTG 71 27 0 37.04 62.089 7.00 0.00 9.089 268 TACATGTGTGTTGTGTATGGATGTAGC 104 27 0 40.74 62.100 6.00 2.00 9.100 269 GGTGTGTCTGTTAAACATGTGTGGTAT 69 27 0 40.74 62.286 7.00 2.00 9.286 270 GTGCATATACATGTGTGTTGTGTATGG 97 27 0 40.74 62.312 6.00 3.00 9.312 271 TGTGGTATTGTGCATATACATGTGTGT 88 27 0 37.04 62.312 6.00 6.00 9.312 272 GTGTGGTATTGTGCATATACATGTGTG 87 27 0 40.74 62.312 6.00 4.00 9.312 273 TGTGTGGTATTGTGCATATACATGTGT 86 27 0 37.04 62.312 6.00 6.00 9.312 274 TGTTAAACATGTGTGGTATTGTGCATA 77 27 0 33.33 62.329 7.00 4.00 9.329 275 TATGGATGTAGCTTCTTGACTGTGTGT 119 27 0 40.74 62.383 4.00 0.00 9.383 276 GTATGGATGTAGCTTCTTGACTGTGTG 118 27 0 44.44 62.383 4.00 0.00 9.383 277 TGTATGGATGTAGCTTCTTGACTGTGT 117 27 0 40.74 62.383 4.00 1.00 9.383 278 GTGTATGGATGTAGCTTCTTGACTGTG 116 27 0 44.44 62.383 4.00 1.00 9.383 279 TGTGTATGGATGTAGCTTCTTGACTGT 115 27 0 40.74 62.383 4.00 3.00 9.383 280 ATGTGTGTTGTGTATGGATGTAGCTTC 107 27 0 40.74 62.494 4.00 4.00 9.494 281 ATGTAGCTTCTTGACTGTGTGTTGTGT 124 27 0 40.74 62.495 4.00 0.00 9.495 282 TGTTGTGTATGGATGTAGCTTCTTGAC 112 27 0 40.74 62.685 4.00 1.00 9.685 283 GTGTTGTGTATGGATGTAGCTTCTTGA 111 27 0 40.74 62.685 4.00 2.00 9.685 284 TGGTATTGTGCATATACATGTGTGTTG 90 27 0 37.04 62.716 6.00 4.00 9.716 285 TTGTGTATGGATGTAGCTTCTTGACTG 114 27 0 40.74 62.778 4.00 1.00 9.778 286 GTCTGTTAAACATGTGTGGTATTGTGC 74 27 0 40.74 62.806 7.00 2.00 9.806 287 CATATGTGTTGTGTATAAATGTTGTGT 25 27 0 29.63 57.112 6.00 0.00 9.888 288 ACATATGTGTTGTGTATAAATGTTGTG 24 27 0 29.63 57.112 8.00 0.00 9.888 289 CACATATGTGTTGTGTATAAATGTTGT 23 27 0 29.63 57.112 10.00 2.00 9.888 290 ACACATATGTGTTGTGTATAAATGTTG 22 27 0 29.63 57.112 12.00 0.00 9.888 291 ACATGTGTGTTGTGTATGGATGTAGCT 105 27 0 40.74 62.902 6.00 4.00 9.902 primer3-2.3.6/test/primer_list_last/AGAG.for0000664000077300007730000001537012231156244017625 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 GAGAGAGAGAGAGAGAGAGAGAGAGA 55 26 0 50.00 57.752 0.00 0.00 8.248 1 GAGAGAGAGAGAGAGAGAGAGAGAGA 53 26 0 50.00 57.752 0.00 0.00 8.248 2 GAGAGAGAGAGAGAGAGAGAGAGAGA 51 26 0 50.00 57.752 0.00 0.00 8.248 3 GAGAGAGAGAGAGAGAGAGAGAGAGA 49 26 0 50.00 57.752 0.00 0.00 8.248 4 GAGAGAGAGAGAGAGAGAGAGAGAGA 47 26 0 50.00 57.752 0.00 0.00 8.248 5 GAGAGAGAGAGAGAGAGAGAGAGAGA 45 26 0 50.00 57.752 0.00 0.00 8.248 6 GAGAGAGAGAGAGAGAGAGAGAGAGA 43 26 0 50.00 57.752 0.00 0.00 8.248 7 GAGAGAGAGAGAGAGAGAGAGAGAGA 41 26 0 50.00 57.752 0.00 0.00 8.248 8 GAGAGAGAGAGAGAGAGAGAGAGAGA 39 26 0 50.00 57.752 0.00 0.00 8.248 9 GAGAGAGAGAGAGAGAGAGAGAGAGA 37 26 0 50.00 57.752 0.00 0.00 8.248 10 GAGAGAGAGAGAGAGAGAGAGAGAGA 35 26 0 50.00 57.752 0.00 0.00 8.248 11 GAGAGAGAGAGAGAGAGAGAGAGAGA 33 26 0 50.00 57.752 0.00 0.00 8.248 12 GAGAGAGAGAGAGAGAGAGAGAGAGA 31 26 0 50.00 57.752 0.00 0.00 8.248 13 GAGAGAGAGAGAGAGAGAGAGAGAGA 29 26 0 50.00 57.752 0.00 0.00 8.248 14 GAGAGAGAGAGAGAGAGAGAGAGAGA 27 26 0 50.00 57.752 0.00 0.00 8.248 15 GAGAGAGAGAGAGAGAGAGAGAGAGA 25 26 0 50.00 57.752 0.00 0.00 8.248 16 GAGAGAGAGAGAGAGAGAGAGAGAGA 23 26 0 50.00 57.752 0.00 0.00 8.248 17 GAGAGAGAGAGAGAGAGAGAGAGAGA 21 26 0 50.00 57.752 0.00 0.00 8.248 18 GAGAGAGAGAGAGAGAGAGAGAGAGA 19 26 0 50.00 57.752 0.00 0.00 8.248 19 GAGAGAGAGAGAGAGAGAGAGAGAGA 17 26 0 50.00 57.752 0.00 0.00 8.248 20 GAGAGAGAGAGAGAGAGAGAGAGAGA 15 26 0 50.00 57.752 0.00 0.00 8.248 21 GAGAGAGAGAGAGAGAGAGAGAGAGA 13 26 0 50.00 57.752 0.00 0.00 8.248 22 GAGAGAGAGAGAGAGAGAGAGAGAGA 11 26 0 50.00 57.752 0.00 0.00 8.248 23 GAGAGAGAGAGAGAGAGAGAGAGAGA 9 26 0 50.00 57.752 0.00 0.00 8.248 24 GAGAGAGAGAGAGAGAGAGAGAGAGA 7 26 0 50.00 57.752 0.00 0.00 8.248 25 GAGAGAGAGAGAGAGAGAGAGAGAGA 5 26 0 50.00 57.752 0.00 0.00 8.248 26 GAGAGAGAGAGAGAGAGAGAGAGAGA 3 26 0 50.00 57.752 0.00 0.00 8.248 27 GAGAGAGAGAGAGAGAGAGAGAGAGA 1 26 0 50.00 57.752 0.00 0.00 8.248 28 GAGAGAGAGAGAGAGAGAGAGAGAGAG 55 27 0 51.85 58.701 0.00 0.00 8.299 29 AGAGAGAGAGAGAGAGAGAGAGAGAGA 54 27 0 48.15 58.701 0.00 0.00 8.299 30 GAGAGAGAGAGAGAGAGAGAGAGAGAG 53 27 0 51.85 58.701 0.00 0.00 8.299 31 AGAGAGAGAGAGAGAGAGAGAGAGAGA 52 27 0 48.15 58.701 0.00 0.00 8.299 32 GAGAGAGAGAGAGAGAGAGAGAGAGAG 51 27 0 51.85 58.701 0.00 0.00 8.299 33 AGAGAGAGAGAGAGAGAGAGAGAGAGA 50 27 0 48.15 58.701 0.00 0.00 8.299 34 GAGAGAGAGAGAGAGAGAGAGAGAGAG 49 27 0 51.85 58.701 0.00 0.00 8.299 35 AGAGAGAGAGAGAGAGAGAGAGAGAGA 48 27 0 48.15 58.701 0.00 0.00 8.299 36 GAGAGAGAGAGAGAGAGAGAGAGAGAG 47 27 0 51.85 58.701 0.00 0.00 8.299 37 AGAGAGAGAGAGAGAGAGAGAGAGAGA 46 27 0 48.15 58.701 0.00 0.00 8.299 38 GAGAGAGAGAGAGAGAGAGAGAGAGAG 45 27 0 51.85 58.701 0.00 0.00 8.299 39 AGAGAGAGAGAGAGAGAGAGAGAGAGA 44 27 0 48.15 58.701 0.00 0.00 8.299 40 GAGAGAGAGAGAGAGAGAGAGAGAGAG 43 27 0 51.85 58.701 0.00 0.00 8.299 41 AGAGAGAGAGAGAGAGAGAGAGAGAGA 42 27 0 48.15 58.701 0.00 0.00 8.299 42 GAGAGAGAGAGAGAGAGAGAGAGAGAG 41 27 0 51.85 58.701 0.00 0.00 8.299 43 AGAGAGAGAGAGAGAGAGAGAGAGAGA 40 27 0 48.15 58.701 0.00 0.00 8.299 44 GAGAGAGAGAGAGAGAGAGAGAGAGAG 39 27 0 51.85 58.701 0.00 0.00 8.299 45 AGAGAGAGAGAGAGAGAGAGAGAGAGA 38 27 0 48.15 58.701 0.00 0.00 8.299 46 GAGAGAGAGAGAGAGAGAGAGAGAGAG 37 27 0 51.85 58.701 0.00 0.00 8.299 47 AGAGAGAGAGAGAGAGAGAGAGAGAGA 36 27 0 48.15 58.701 0.00 0.00 8.299 48 GAGAGAGAGAGAGAGAGAGAGAGAGAG 35 27 0 51.85 58.701 0.00 0.00 8.299 49 AGAGAGAGAGAGAGAGAGAGAGAGAGA 34 27 0 48.15 58.701 0.00 0.00 8.299 50 GAGAGAGAGAGAGAGAGAGAGAGAGAG 33 27 0 51.85 58.701 0.00 0.00 8.299 51 AGAGAGAGAGAGAGAGAGAGAGAGAGA 32 27 0 48.15 58.701 0.00 0.00 8.299 52 GAGAGAGAGAGAGAGAGAGAGAGAGAG 31 27 0 51.85 58.701 0.00 0.00 8.299 53 AGAGAGAGAGAGAGAGAGAGAGAGAGA 30 27 0 48.15 58.701 0.00 0.00 8.299 54 GAGAGAGAGAGAGAGAGAGAGAGAGAG 29 27 0 51.85 58.701 0.00 0.00 8.299 55 AGAGAGAGAGAGAGAGAGAGAGAGAGA 28 27 0 48.15 58.701 0.00 0.00 8.299 56 GAGAGAGAGAGAGAGAGAGAGAGAGAG 27 27 0 51.85 58.701 0.00 0.00 8.299 57 AGAGAGAGAGAGAGAGAGAGAGAGAGA 26 27 0 48.15 58.701 0.00 0.00 8.299 58 GAGAGAGAGAGAGAGAGAGAGAGAGAG 25 27 0 51.85 58.701 0.00 0.00 8.299 59 AGAGAGAGAGAGAGAGAGAGAGAGAGA 24 27 0 48.15 58.701 0.00 0.00 8.299 60 GAGAGAGAGAGAGAGAGAGAGAGAGAG 23 27 0 51.85 58.701 0.00 0.00 8.299 61 AGAGAGAGAGAGAGAGAGAGAGAGAGA 22 27 0 48.15 58.701 0.00 0.00 8.299 62 GAGAGAGAGAGAGAGAGAGAGAGAGAG 21 27 0 51.85 58.701 0.00 0.00 8.299 63 AGAGAGAGAGAGAGAGAGAGAGAGAGA 20 27 0 48.15 58.701 0.00 0.00 8.299 64 GAGAGAGAGAGAGAGAGAGAGAGAGAG 19 27 0 51.85 58.701 0.00 0.00 8.299 65 AGAGAGAGAGAGAGAGAGAGAGAGAGA 18 27 0 48.15 58.701 0.00 0.00 8.299 66 GAGAGAGAGAGAGAGAGAGAGAGAGAG 17 27 0 51.85 58.701 0.00 0.00 8.299 67 AGAGAGAGAGAGAGAGAGAGAGAGAGA 16 27 0 48.15 58.701 0.00 0.00 8.299 68 GAGAGAGAGAGAGAGAGAGAGAGAGAG 15 27 0 51.85 58.701 0.00 0.00 8.299 69 AGAGAGAGAGAGAGAGAGAGAGAGAGA 14 27 0 48.15 58.701 0.00 0.00 8.299 70 GAGAGAGAGAGAGAGAGAGAGAGAGAG 13 27 0 51.85 58.701 0.00 0.00 8.299 71 AGAGAGAGAGAGAGAGAGAGAGAGAGA 12 27 0 48.15 58.701 0.00 0.00 8.299 72 GAGAGAGAGAGAGAGAGAGAGAGAGAG 11 27 0 51.85 58.701 0.00 0.00 8.299 73 AGAGAGAGAGAGAGAGAGAGAGAGAGA 10 27 0 48.15 58.701 0.00 0.00 8.299 74 GAGAGAGAGAGAGAGAGAGAGAGAGAG 9 27 0 51.85 58.701 0.00 0.00 8.299 75 AGAGAGAGAGAGAGAGAGAGAGAGAGA 8 27 0 48.15 58.701 0.00 0.00 8.299 76 GAGAGAGAGAGAGAGAGAGAGAGAGAG 7 27 0 51.85 58.701 0.00 0.00 8.299 77 AGAGAGAGAGAGAGAGAGAGAGAGAGA 6 27 0 48.15 58.701 0.00 0.00 8.299 78 GAGAGAGAGAGAGAGAGAGAGAGAGAG 5 27 0 51.85 58.701 0.00 0.00 8.299 79 AGAGAGAGAGAGAGAGAGAGAGAGAGA 4 27 0 48.15 58.701 0.00 0.00 8.299 80 GAGAGAGAGAGAGAGAGAGAGAGAGAG 3 27 0 51.85 58.701 0.00 0.00 8.299 81 AGAGAGAGAGAGAGAGAGAGAGAGAGA 2 27 0 48.15 58.701 0.00 0.00 8.299 82 GAGAGAGAGAGAGAGAGAGAGAGAGAG 1 27 0 51.85 58.701 0.00 0.00 8.299 83 AGAGAGAGAGAGAGAGAGAGAGAGAGA 0 27 0 48.15 58.701 0.00 0.00 8.299 primer3-2.3.6/test/primer_list_last/SP01-03.txt.for0000664000077300007730000002213012231156244020637 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TTAGAAGGGGTGTCTGGGTG 72 20 0 55.00 59.959 3.00 0.00 0.041 1 CTGCAGGTTTAGAAGGGGTG 64 20 0 55.00 59.728 6.00 0.00 0.272 2 CACCCTGCAGGTTTAGAAGG 60 20 0 55.00 59.728 8.00 0.00 0.272 3 AGCACACCCTGCAGGTTTAG 56 20 0 55.00 60.312 8.00 6.00 0.312 4 TGCAGGTTTAGAAGGGGTGT 65 20 0 50.00 59.592 4.00 0.00 0.408 5 CCTGCAGGTTTAGAAGGGGT 63 20 0 55.00 60.488 8.00 0.00 0.488 6 ACCCTGCAGGTTTAGAAGGG 61 20 0 55.00 60.488 8.00 2.00 0.488 7 CACCCTGTAAACCCTGGCT 7 19 0 57.89 59.974 3.00 3.00 1.026 8 TCTGCACCCTGTAAACCCTG 3 20 0 55.00 61.084 4.00 1.00 1.084 9 CACACCCTGCAGGTTTAGAAG 58 21 0 52.38 59.782 8.00 2.00 1.218 10 CACACCCTGCAGGTTTAGAA 58 20 0 50.00 58.773 8.00 2.00 1.227 11 GCACACCCTGCAGGTTTAGA 57 20 0 55.00 61.236 8.00 4.00 1.236 12 CACTGAGGTCAGCACACCC 46 19 0 63.16 60.311 6.00 2.00 1.311 13 CCACTGAGGTCAGCACACC 45 19 0 63.16 60.311 6.00 4.00 1.311 14 CCCACTGAGGTCAGCACAC 44 19 0 63.16 60.311 6.00 2.00 1.311 15 CCTGCAGGTTTAGAAGGGG 63 19 0 57.89 59.673 8.00 0.00 1.327 16 CCCTGCAGGTTTAGAAGGG 62 19 0 57.89 59.673 8.00 2.00 1.327 17 GCAGGTTTAGAAGGGGTGTC 66 20 0 55.00 58.646 2.00 1.00 1.354 18 ACTGAGGTCAGCACACCCTG 47 20 0 60.00 61.355 6.00 2.00 1.355 19 CACTGAGGTCAGCACACCCT 46 20 0 60.00 61.355 6.00 2.00 1.355 20 GCAGGTTTAGAAGGGGTGTCT 66 21 0 52.38 59.630 3.00 0.00 1.370 21 CTGAGGTCAGCACACCCTG 48 19 0 63.16 60.461 6.00 2.00 1.461 22 ATCTGCACCCTGTAAACCCT 2 20 0 50.00 58.525 4.00 0.00 1.475 23 TTTAGAAGGGGTGTCTGGGT 71 20 0 50.00 58.498 3.00 0.00 1.502 24 GTTTAGAAGGGGTGTCTGGG 70 20 0 55.00 58.498 3.00 0.00 1.502 25 GGTTTAGAAGGGGTGTCTGG 69 20 0 55.00 58.498 3.00 0.00 1.502 26 AGGTTTAGAAGGGGTGTCTGG 68 21 0 52.38 59.488 3.00 0.00 1.512 27 CTGCAGGTTTAGAAGGGGTGT 64 21 0 52.38 60.534 6.00 0.00 1.534 28 ACACCCTGCAGGTTTAGAAGG 59 21 0 52.38 60.534 8.00 0.00 1.534 29 GTTTAGAAGGGGTGTCTGGGT 70 21 0 52.38 59.355 3.00 0.00 1.645 30 GCACACCCTGCAGGTTTAG 57 19 0 57.89 59.303 8.00 6.00 1.697 31 AGCACACCCTGCAGGTTTA 56 19 0 52.63 59.303 8.00 4.00 1.697 32 CACCCTGTAAACCCTGGCTC 7 20 0 60.00 61.826 3.00 1.00 1.826 33 CCTGTAAACCCTGGCTCCT 10 19 0 57.89 59.148 3.00 0.00 1.852 34 CTGCACCCTGTAAACCCTG 4 19 0 57.89 59.146 4.00 1.00 1.854 35 CCTGTAAACCCTGGCTCCTG 10 20 0 60.00 61.944 3.00 2.00 1.944 36 GACACCCCACTGAGGTCAG 39 19 0 63.16 59.044 7.00 6.00 1.956 37 ACCCTGTAAACCCTGGCTC 8 19 0 57.89 59.003 3.00 1.00 1.997 38 CTGTAAACCCTGGCTCCTGC 11 20 0 60.00 62.090 3.00 2.00 2.090 39 ACCCCACTGAGGTCAGCAC 42 19 0 63.16 61.171 7.00 1.00 2.171 40 ACACCCCACTGAGGTCAGC 40 19 0 63.16 61.171 7.00 4.00 2.171 41 TGTAAACCCTGGCTCCTGC 12 19 0 57.89 61.192 3.00 2.00 2.192 42 CAGCACACCCTGCAGGTTTA 55 20 0 55.00 62.212 8.00 4.00 2.212 43 CCCCACTGAGGTCAGCAC 43 18 0 66.67 60.252 6.00 1.00 2.252 44 ACCCCACTGAGGTCAGCA 42 18 0 61.11 60.252 7.00 2.00 2.252 45 CACCCCACTGAGGTCAGC 41 18 0 66.67 60.252 7.00 4.00 2.252 46 TTTAGAAGGGGTGTCTGGGTG 71 21 0 52.38 61.267 3.00 0.00 2.267 47 TGCAGGTTTAGAAGGGGTG 65 19 0 52.63 58.707 4.00 0.00 2.293 48 CAGGTTTAGAAGGGGTGTCTG 67 21 0 52.38 58.705 3.00 1.00 2.295 49 ATCTGCACCCTGTAAACCCTG 2 21 0 52.38 61.297 4.00 1.00 2.297 50 TAGAAGGGGTGTCTGGGTGC 73 20 0 60.00 62.397 3.00 2.00 2.397 51 TGCAGGTTTAGAAGGGGTGTC 65 21 0 52.38 61.411 4.00 1.00 2.411 52 GAGGTCAGCACACCCTGC 50 18 0 66.67 60.425 4.00 2.00 2.425 53 TAGAAGGGGTGTCTGGGTG 73 19 0 57.89 58.495 3.00 0.00 2.505 54 ACCCTGTAAACCCTGGCTCC 8 20 0 60.00 62.523 3.00 2.00 2.523 55 CCCTGTAAACCCTGGCTCCT 9 20 0 60.00 62.632 3.00 0.00 2.632 56 TGAGGTCAGCACACCCTG 49 18 0 61.11 59.315 4.00 1.00 2.685 57 CCCACTGAGGTCAGCACA 44 18 0 61.11 59.315 6.00 3.00 2.685 58 CTGTAAACCCTGGCTCCTG 11 19 0 57.89 58.300 3.00 2.00 2.700 59 AGCACACCCTGCAGGTTT 56 18 0 55.56 59.261 8.00 6.00 2.739 60 CTGCACCCTGTAAACCCTGG 4 20 0 60.00 62.773 4.00 3.00 2.773 61 GTAAACCCTGGCTCCTGCCT 13 20 0 60.00 62.773 3.00 0.00 2.773 62 ACTGAGGTCAGCACACCCT 47 19 0 57.89 58.217 6.00 2.00 2.783 63 CCCTGTAAACCCTGGCTCC 9 19 0 63.16 61.789 3.00 2.00 2.789 64 GGGACACCCCACTGAGGT 37 18 0 66.67 60.812 7.00 7.00 2.812 65 TCTGCACCCTGTAAACCCT 3 19 0 52.63 58.144 4.00 0.00 2.856 66 TGCACCCTGTAAACCCTGG 5 19 0 57.89 61.900 4.00 3.00 2.900 67 CAGCACACCCTGCAGGTT 55 18 0 61.11 60.905 8.00 8.00 2.905 68 GTAAACCCTGGCTCCTGCC 13 19 0 63.16 61.937 3.00 2.00 2.937 69 GGTTTAGAAGGGGTGTCTGGG 69 21 0 57.14 61.965 3.00 0.00 2.965 70 GGACACCCCACTGAGGTCAG 38 20 0 65.00 62.981 7.00 6.00 2.981 71 GGACACCCCACTGAGGTCA 38 19 0 63.16 62.043 7.00 4.00 3.043 72 TAAACCCTGGCTCCTGCCT 14 19 0 57.89 62.055 3.00 0.00 3.055 73 CACCCTGTAAACCCTGGC 7 18 0 61.11 58.930 3.00 2.00 3.070 74 GCACCCTGTAAACCCTGG 6 18 0 61.11 58.930 3.00 3.00 3.070 75 AGCACACCCTGCAGGTTTAGA 56 21 0 52.38 62.124 8.00 4.00 3.124 76 TAAACCCTGGCTCCTGCC 14 18 0 61.11 61.147 3.00 2.00 3.147 77 GGACACCCCACTGAGGTC 38 18 0 66.67 58.806 7.00 5.00 3.194 78 CAGCACACCCTGCAGGTTT 55 19 0 57.89 62.292 8.00 6.00 3.292 79 GTCAGCACACCCTGCAGGT 53 19 0 63.16 62.397 8.00 6.00 3.397 80 TTTAGAAGGGGTGTCTGGG 71 19 0 52.63 57.569 3.00 0.00 3.431 81 GCACACCCTGCAGGTTTAGAA 57 21 0 52.38 62.477 8.00 2.00 3.477 82 AGAAGGGGTGTCTGGGTGC 74 19 0 63.16 62.484 3.00 2.00 3.484 83 AGGTCAGCACACCCTGCAG 51 19 0 63.16 62.524 6.00 6.00 3.524 84 TCAGCACACCCTGCAGGT 54 18 0 61.11 61.534 8.00 6.00 3.534 85 GTCAGCACACCCTGCAGG 53 18 0 66.67 61.534 8.00 8.00 3.534 86 GGTCAGCACACCCTGCAG 52 18 0 66.67 61.534 6.00 6.00 3.534 87 AGGTCAGCACACCCTGCA 51 18 0 61.11 61.534 4.00 4.00 3.534 88 GAAGGGGTGTCTGGGTGC 75 18 0 66.67 61.536 2.00 2.00 3.536 89 ATCTGCACCCTGTAAACCC 2 19 0 52.63 57.435 4.00 0.00 3.565 90 AGAAGGGGTGTCTGGGTG 74 18 0 61.11 58.416 3.00 0.00 3.584 91 GTAAACCCTGGCTCCTGC 13 18 0 61.11 58.230 3.00 2.00 3.770 92 CACACCCTGCAGGTTTAGA 58 19 0 52.63 57.225 8.00 3.00 3.775 93 GCACCCTGTAAACCCTGGC 6 19 0 63.16 62.779 3.00 2.00 3.779 94 GCACACCCTGCAGGTTTA 57 18 0 55.56 58.186 8.00 4.00 3.814 95 GGGACACCCCACTGAGGTC 37 19 0 68.42 62.829 7.00 5.00 3.829 96 TCAGCACACCCTGCAGGTT 54 19 0 57.89 62.920 8.00 8.00 3.920 97 CCTGTAAACCCTGGCTCC 10 18 0 61.11 58.071 3.00 2.00 3.929 98 CCCTGTAAACCCTGGCTC 9 18 0 61.11 58.071 3.00 1.00 3.929 99 TGCACCCTGTAAACCCTG 5 18 0 55.56 58.020 4.00 1.00 3.980 100 GTTTAGAAGGGGTGTCTGGGTG 70 22 0 54.55 61.999 3.00 0.00 3.999 101 CAGGTTTAGAAGGGGTGTCTGG 67 22 0 54.55 62.108 3.00 0.00 4.108 102 AAACCCTGGCTCCTGCCT 15 18 0 61.11 62.126 3.00 0.00 4.126 103 GACACCCCACTGAGGTCA 39 18 0 61.11 57.806 7.00 4.00 4.194 104 GCAGGTTTAGAAGGGGTGTCTG 66 22 0 54.55 62.245 3.00 1.00 4.245 105 TGCAGGTTTAGAAGGGGTGTCT 65 22 0 50.00 62.245 4.00 0.00 4.245 106 CTGCAGGTTTAGAAGGGGTGTC 64 22 0 54.55 62.245 6.00 1.00 4.245 107 ACCCTGGCTCCTGCCTCT 17 18 0 66.67 62.344 3.00 0.00 4.344 108 GGTTTAGAAGGGGTGTCTGGGT 69 22 0 54.55 62.650 3.00 0.00 4.650 109 AACCCTGGCTCCTGCCTC 16 18 0 66.67 62.728 3.00 0.00 4.728 110 AGGTTTAGAAGGGGTGTCTGGG 68 22 0 54.55 62.752 3.00 0.00 4.752 111 CTGAGGTCAGCACACCCT 48 18 0 61.11 57.145 6.00 2.00 4.855 112 TGTAAACCCTGGCTCCTG 12 18 0 55.56 57.142 3.00 2.00 4.858 113 ACCCTGTAAACCCTGGCT 8 18 0 55.56 57.001 3.00 3.00 4.999 primer3-2.3.6/test/primer_list_last/included_test.for0000664000077300007730000005577012231156244021764 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGTGTCTTGTGGGTGTGTCTG 78 21 0 52.38 60.680 1.00 1.00 1.680 1 TGTCTTGTGGGTGTGTCTGTT 80 21 0 47.62 59.059 1.00 0.00 1.941 2 GTGTCTTGTGGGTGTGTCTGTT 79 22 0 50.00 59.972 1.00 0.00 2.028 3 GAACATGTGTGGCATTGTGT 20 20 0 45.00 57.775 6.00 0.00 2.225 4 GTGTCTTGTGGGTGTGTCTGT 79 21 0 52.38 58.472 1.00 0.00 2.528 5 TGTCTTGTGGGTGTGTCTGT 80 20 0 50.00 57.431 1.00 0.00 2.569 6 GTGTCTTGTGGGTGTGTCTG 79 20 0 55.00 57.431 1.00 1.00 2.569 7 TGTGTCTTGTGGGTGTGTCT 78 20 0 50.00 57.431 1.00 0.00 2.569 8 TGTCTTGTGGGTGTGTCTGTTA 80 22 0 45.45 59.108 2.00 2.00 2.892 9 TTGTGGGTGTGTCTGTTAAACA 84 22 0 40.91 58.997 7.00 7.00 3.003 10 GTGTCTTGTGGGTGTGTCTGTTA 79 23 0 47.83 59.984 2.00 2.00 3.016 11 CTTGTGGGTGTGTCTGTTAAACA 83 23 0 43.48 59.981 7.00 7.00 3.019 12 GAACATGTGTGGCATTGTGTA 20 21 0 42.86 57.880 6.00 2.00 3.120 13 TGTCTTGTGGGTGTGTCTGTTAA 80 23 0 43.48 60.493 4.00 4.00 3.493 14 TGTGGGTGTGTCTGTTAAACA 85 21 0 42.86 57.489 7.00 7.00 3.511 15 TGTGTCTTGTGGGTGTGTCTGT 78 22 0 50.00 61.549 1.00 0.00 3.549 16 AAACATGTGTGGTATTGTGCA 101 21 0 38.10 57.391 6.00 4.00 3.609 17 TTGTGGGTGTGTCTGTTAAACAT 84 23 0 39.13 59.319 7.00 5.00 3.681 18 GAACATGTGTGGCATTGTGTAT 20 22 0 40.91 58.283 6.00 2.00 3.717 19 TGGGTGTGTCTGTTAAACATGTG 87 23 0 43.48 60.746 7.00 4.00 3.746 20 TGTGGGTGTGTCTGTTAAACATG 85 23 0 43.48 60.746 7.00 5.00 3.746 21 TCTTGTGGGTGTGTCTGTTAAA 82 22 0 40.91 58.187 4.00 3.00 3.813 22 AGCTTCTTGACTGTGTGTTGTG 148 22 0 45.45 58.075 4.00 0.00 3.925 23 TCTTGTGGGTGTGTCTGTTAAAC 82 23 0 43.48 59.063 5.00 5.00 3.937 24 GTCTTGTGGGTGTGTCTGTTAAA 81 23 0 43.48 59.063 4.00 3.00 3.937 25 TGGATGTAGCTTCTTGACTGTG 141 22 0 45.45 58.036 4.00 1.00 3.964 26 AGCTTCTTGACTGTGTGTTGTGT 148 23 0 43.48 58.981 4.00 0.00 4.019 27 GGATGTAGCTTCTTGACTGTGTG 142 23 0 47.83 58.925 4.00 0.00 4.075 28 TGGATGTAGCTTCTTGACTGTGT 141 23 0 43.48 58.925 4.00 1.00 4.075 29 TTAAACATGTGTGGTATTGTGCA 99 23 0 34.78 58.925 6.00 4.00 4.075 30 GGGTGTGTCTGTTAAACATGTG 88 22 0 45.45 57.912 7.00 4.00 4.088 31 TGGGTGTGTCTGTTAAACATGT 87 22 0 40.91 57.912 7.00 6.00 4.088 32 GTGGGTGTGTCTGTTAAACATG 86 22 0 45.45 57.912 7.00 5.00 4.088 33 TGTGGGTGTGTCTGTTAAACAT 85 22 0 40.91 57.912 7.00 5.00 4.088 34 AACATGTGTGGCATTGTGTATACA 21 24 0 37.50 60.103 8.00 8.00 4.103 35 GTCTTGTGGGTGTGTCTGTTAAAC 81 24 0 45.83 59.875 5.00 5.00 4.125 36 CATGTGTGGCATTGTGTATACA 23 22 0 40.91 57.850 8.00 8.00 4.150 37 AAACATGTGTGGTATTGTGCAT 101 22 0 36.36 57.811 6.00 2.00 4.189 38 GGGTGTGTCTGTTAAACATGTGT 88 23 0 43.48 58.808 7.00 6.00 4.192 39 GTGGGTGTGTCTGTTAAACATGT 86 23 0 43.48 58.808 7.00 6.00 4.192 40 ACATGTGTGGCATTGTGTATACA 22 23 0 39.13 58.766 8.00 8.00 4.234 41 CTTGTGGGTGTGTCTGTTAAACAT 83 24 0 41.67 60.239 7.00 5.00 4.239 42 GGATGTAGCTTCTTGACTGTGTGT 142 24 0 45.83 59.748 4.00 0.00 4.252 43 GTTAAACATGTGTGGTATTGTGCA 98 24 0 37.50 59.733 6.00 4.00 4.267 44 TGTTAAACATGTGTGGTATTGTGC 97 24 0 37.50 59.733 7.00 2.00 4.267 45 GTCTTGTGGGTGTGTCTGTTAA 81 22 0 45.45 57.635 4.00 4.00 4.365 46 TAAACATGTGTGGTATTGTGCA 100 22 0 36.36 57.504 6.00 4.00 4.496 47 TGTGTGTTGTGTATGGATGTAGC 128 23 0 43.48 58.499 2.00 2.00 4.501 48 TGTGTGTTGTGTATGGATGTAGCT 128 24 0 41.67 59.493 4.00 4.00 4.507 49 ATGGATGTAGCTTCTTGACTGTG 140 23 0 43.48 58.408 4.00 1.00 4.592 50 GAACATGTGTGGCATTGTGTATA 20 23 0 39.13 58.360 6.00 4.00 4.640 51 TTGTGCATATACATGTGTGTTGTG 115 24 0 37.50 59.345 6.00 2.00 4.655 52 ATGGATGTAGCTTCTTGACTGTGT 140 24 0 41.67 59.238 4.00 1.00 4.762 53 TTAAACATGTGTGGTATTGTGCAT 99 24 0 33.33 59.233 6.00 2.00 4.767 54 GAACATGTGTGGCATTGTGTATAC 20 24 0 41.67 59.208 6.00 6.00 4.792 55 CTTGTGGGTGTGTCTGTTAAAC 83 22 0 45.45 57.176 5.00 5.00 4.824 56 TAGCTTCTTGACTGTGTGTTGTG 147 23 0 43.48 58.160 4.00 0.00 4.840 57 TGTAGCTTCTTGACTGTGTGTTG 145 23 0 43.48 58.160 4.00 0.00 4.840 58 TGTGTTGTGTATGGATGTAGCTTC 130 24 0 41.67 59.091 4.00 4.00 4.909 59 TAGCTTCTTGACTGTGTGTTGTGT 147 24 0 41.67 59.030 4.00 0.00 4.970 60 GTAGCTTCTTGACTGTGTGTTGTG 146 24 0 45.83 59.030 4.00 0.00 4.970 61 TGTAGCTTCTTGACTGTGTGTTGT 145 24 0 41.67 59.030 4.00 0.00 4.970 62 TGTGTCTGTTAAACATGTGTGG 91 22 0 40.91 57.024 7.00 2.00 4.976 63 TGTGTTGTGTATGGATGTAGCTTCT 130 25 0 40.00 60.002 4.00 2.00 5.002 64 GTTAAACATGTGTGGTATTGTGCAT 98 25 0 36.00 59.991 6.00 2.00 5.009 65 TGTGTCTGTTAAACATGTGTGGT 91 23 0 39.13 57.983 7.00 0.00 5.017 66 GTGTGTCTGTTAAACATGTGTGG 90 23 0 43.48 57.983 7.00 2.00 5.017 67 GGTGTGTCTGTTAAACATGTGTG 89 23 0 43.48 57.983 7.00 4.00 5.017 68 TGTGCATATACATGTGTGTTGTG 116 23 0 39.13 57.923 6.00 2.00 5.077 69 AAACATGTGTGGTATTGTGCATA 101 23 0 34.78 57.903 6.00 4.00 5.097 70 TAAACATGTGTGGTATTGTGCAT 100 23 0 34.78 57.903 6.00 2.00 5.097 71 GTGTGTTGTGTATGGATGTAGCTTC 129 25 0 44.00 59.880 4.00 4.00 5.120 72 GTGTGTCTGTTAAACATGTGTGGT 90 24 0 41.67 58.868 7.00 0.00 5.132 73 TTGTGCATATACATGTGTGTTGTGT 115 25 0 36.00 60.151 6.00 0.00 5.151 74 ATGTGTGTTGTGTATGGATGTAGC 127 24 0 41.67 58.842 2.00 2.00 5.158 75 GTAGCTTCTTGACTGTGTGTTGTGT 146 25 0 44.00 59.835 4.00 0.00 5.165 76 TTGTGTATGGATGTAGCTTCTTGA 134 24 0 37.50 58.834 4.00 2.00 5.166 77 TGTGCATATACATGTGTGTTGTGT 116 24 0 37.50 58.828 6.00 0.00 5.172 78 ATGTGTGTTGTGTATGGATGTAGCT 127 25 0 40.00 59.772 4.00 4.00 5.228 79 GTGTCTTGTGGGTGTGTCTGTTAA 79 24 0 45.83 61.270 4.00 4.00 5.270 80 GATGTAGCTTCTTGACTGTGTGTTG 143 25 0 44.00 60.272 4.00 0.00 5.272 81 ACATGTGTGTTGTGTATGGATGT 125 23 0 39.13 57.646 6.00 0.00 5.354 82 ATTGTGCATATACATGTGTGTTGTG 114 25 0 36.00 59.637 6.00 2.00 5.363 83 TTGTGTATGGATGTAGCTTCTTGAC 134 25 0 40.00 59.615 4.00 1.00 5.385 84 GTTGTGTATGGATGTAGCTTCTTGA 133 25 0 40.00 59.615 4.00 2.00 5.385 85 TGTTGTGTATGGATGTAGCTTCTTG 132 25 0 40.00 60.458 4.00 2.00 5.458 86 ATGTAGCTTCTTGACTGTGTGTTG 144 24 0 41.67 58.520 4.00 0.00 5.480 87 GGTGTGTCTGTTAAACATGTGTGG 89 24 0 45.83 61.509 7.00 2.00 5.509 88 GGGTGTGTCTGTTAAACATGTGTG 88 24 0 45.83 61.509 7.00 4.00 5.509 89 TGGGTGTGTCTGTTAAACATGTGT 87 24 0 41.67 61.509 7.00 6.00 5.509 90 GTGGGTGTGTCTGTTAAACATGTG 86 24 0 45.83 61.509 7.00 4.00 5.509 91 TGTGGGTGTGTCTGTTAAACATGT 85 24 0 41.67 61.509 7.00 6.00 5.509 92 TATGGATGTAGCTTCTTGACTGTG 139 24 0 41.67 58.476 4.00 1.00 5.524 93 TGTATGGATGTAGCTTCTTGACTG 137 24 0 41.67 58.476 4.00 1.00 5.524 94 TGTGTATGGATGTAGCTTCTTGA 135 23 0 39.13 57.467 4.00 2.00 5.533 95 CTGTTAAACATGTGTGGTATTGTGC 96 25 0 40.00 60.599 7.00 2.00 5.599 96 TGGATGTAGCTTCTTGACTGTGTG 141 24 0 45.83 61.619 4.00 0.00 5.619 97 AACATGTGTGGCATTGTGTATAC 21 23 0 39.13 57.373 6.00 6.00 5.627 98 TGTGTATGGATGTAGCTTCTTGAC 135 24 0 41.67 58.339 4.00 1.00 5.661 99 ATGTAGCTTCTTGACTGTGTGTTGT 144 25 0 40.00 59.333 4.00 0.00 5.667 100 TGGTATTGTGCATATACATGTGTG 110 24 0 37.50 58.315 6.00 4.00 5.685 101 TGTGGTATTGTGCATATACATGTG 108 24 0 37.50 58.315 6.00 4.00 5.685 102 TGTGTGGTATTGTGCATATACATG 106 24 0 37.50 58.315 6.00 6.00 5.685 103 CATGTGTGGTATTGTGCATATACA 104 24 0 37.50 58.315 6.00 6.00 5.685 104 TATGGATGTAGCTTCTTGACTGTGT 139 25 0 40.00 59.274 4.00 1.00 5.726 105 GTATGGATGTAGCTTCTTGACTGTG 138 25 0 44.00 59.274 4.00 1.00 5.726 106 TGTATGGATGTAGCTTCTTGACTGT 137 25 0 40.00 59.274 4.00 3.00 5.726 107 GTGTATGGATGTAGCTTCTTGACTG 136 25 0 44.00 59.274 4.00 1.00 5.726 108 TGTGTATGGATGTAGCTTCTTGACT 135 25 0 40.00 59.274 4.00 1.00 5.726 109 AGCTTCTTGACTGTGTGTTGTGTG 148 24 0 45.83 61.730 4.00 0.00 5.730 110 TTAAACATGTGTGGTATTGTGCATA 99 25 0 32.00 59.268 6.00 4.00 5.732 111 TCTTGTGGGTGTGTCTGTTAAACA 82 24 0 41.67 61.733 7.00 7.00 5.733 112 TGTCTTGTGGGTGTGTCTGTTAAA 80 24 0 41.67 61.733 4.00 3.00 5.733 113 AAACATGTGTGGTATTGTGCATAT 101 24 0 33.33 58.262 6.00 4.00 5.738 114 TGTGTGTTGTGTATGGATGTAGCTT 128 25 0 40.00 60.742 4.00 2.00 5.742 115 TGTGTTGTGTATGGATGTAGCTT 130 23 0 39.13 57.257 4.00 2.00 5.743 116 TGTGTCTTGTGGGTGTGTCTGTT 78 23 0 47.83 62.831 1.00 0.00 5.831 117 GTGTGTTGTGTATGGATGTAGCTT 129 24 0 41.67 58.137 4.00 2.00 5.863 118 GCATATACATGTGTGTTGTGTATGG 119 25 0 40.00 59.136 6.00 3.00 5.864 119 TGGTATTGTGCATATACATGTGTGT 110 25 0 36.00 59.136 6.00 6.00 5.864 120 GTGGTATTGTGCATATACATGTGTG 109 25 0 40.00 59.136 6.00 4.00 5.864 121 TGTGGTATTGTGCATATACATGTGT 108 25 0 36.00 59.136 6.00 6.00 5.864 122 GTGTGGTATTGTGCATATACATGTG 107 25 0 40.00 59.136 6.00 4.00 5.864 123 TGTGTGGTATTGTGCATATACATGT 106 25 0 36.00 59.136 6.00 6.00 5.864 124 ACATGTGTGGTATTGTGCATATACA 103 25 0 36.00 59.136 6.00 6.00 5.864 125 TGTGTCTGTTAAACATGTGTGGTA 91 24 0 37.50 58.069 7.00 2.00 5.931 126 GTTAAACATGTGTGGTATTGTGC 98 23 0 39.13 57.060 6.00 2.00 5.940 127 GGATGTAGCTTCTTGACTGTGTGTT 142 25 0 44.00 60.962 4.00 0.00 5.962 128 TTGTGTATGGATGTAGCTTCTTG 134 23 0 39.13 57.036 4.00 2.00 5.964 129 TTGTGGGTGTGTCTGTTAAACATG 84 24 0 41.67 61.967 7.00 5.00 5.967 130 GTTAAACATGTGTGGTATTGTGCATA 98 26 0 34.62 60.001 6.00 4.00 6.001 131 TAAACATGTGTGGTATTGTGCATA 100 24 0 33.33 57.988 6.00 4.00 6.012 132 GTATGGATGTAGCTTCTTGACTGTGT 138 26 0 42.31 60.018 4.00 1.00 6.018 133 GTGTATGGATGTAGCTTCTTGACTGT 136 26 0 42.31 60.018 4.00 3.00 6.018 134 GTGTGTCTGTTAAACATGTGTGGTA 90 25 0 40.00 58.919 7.00 2.00 6.081 135 TCTGTTAAACATGTGTGGTATTGTG 95 25 0 36.00 58.912 7.00 0.00 6.088 136 TGTCTGTTAAACATGTGTGGTATTG 93 25 0 36.00 58.912 7.00 0.00 6.088 137 GTTGTGTATGGATGTAGCTTCTTG 133 24 0 41.67 57.904 4.00 2.00 6.096 138 TGTTGTGTATGGATGTAGCTTCTT 132 24 0 37.50 57.904 4.00 4.00 6.096 139 GTGGTATTGTGCATATACATGTGTGT 109 26 0 38.46 59.900 6.00 6.00 6.100 140 GTGTGGTATTGTGCATATACATGTGT 107 26 0 38.46 59.900 6.00 6.00 6.100 141 CATGTGTGTTGTGTATGGATGTAG 126 24 0 41.67 57.896 4.00 0.00 6.104 142 TGTGCATATACATGTGTGTTGTGTA 116 25 0 36.00 58.882 6.00 2.00 6.118 143 TTGTGCATATACATGTGTGTTGTGTA 115 26 0 34.62 60.156 6.00 2.00 6.156 144 ACATGTGTGTTGTGTATGGATGTAG 125 25 0 40.00 58.761 6.00 0.00 6.239 145 ACATGTGTGTTGTGTATGGATGTA 125 24 0 37.50 57.747 6.00 2.00 6.253 146 TACATGTGTGTTGTGTATGGATGT 124 24 0 37.50 57.747 6.00 0.00 6.253 147 GTGTTGTGTATGGATGTAGCTTCTT 131 25 0 40.00 58.712 4.00 4.00 6.288 148 GTCTGTTAAACATGTGTGGTATTGTG 94 26 0 38.46 59.680 7.00 0.00 6.320 149 TGTCTGTTAAACATGTGTGGTATTGT 93 26 0 34.62 59.680 7.00 0.00 6.320 150 GTGTCTGTTAAACATGTGTGGTATTG 92 26 0 38.46 59.680 7.00 0.00 6.320 151 TGTGTCTGTTAAACATGTGTGGTATT 91 26 0 34.62 59.680 7.00 1.00 6.320 152 TATTGTGCATATACATGTGTGTTGTG 113 26 0 34.62 59.659 6.00 2.00 6.341 153 GTTGTGTATGGATGTAGCTTCTTGAC 133 26 0 42.31 60.343 4.00 1.00 6.343 154 GGTATTGTGCATATACATGTGTGTTG 111 26 0 38.46 60.346 6.00 4.00 6.346 155 TGGTATTGTGCATATACATGTGTGTT 110 26 0 34.62 60.346 6.00 4.00 6.346 156 AACATGTGTGGTATTGTGCATATACA 102 26 0 34.62 60.346 6.00 6.00 6.346 157 ATGTGTGGTATTGTGCATATACATG 105 25 0 36.00 58.650 6.00 6.00 6.350 158 CATGTGTGGTATTGTGCATATACAT 104 25 0 36.00 58.650 6.00 4.00 6.350 159 GATGTAGCTTCTTGACTGTGTGTT 143 24 0 41.67 57.626 4.00 0.00 6.374 160 ATTGTGCATATACATGTGTGTTGTGT 114 26 0 34.62 60.393 6.00 0.00 6.393 161 TTGTGTATGGATGTAGCTTCTTGACT 134 26 0 38.46 60.455 4.00 1.00 6.455 162 TTAAACATGTGTGGTATTGTGCATAT 99 26 0 30.77 59.536 6.00 4.00 6.464 163 CATGTGTGTTGTGTATGGATGTAGC 126 25 0 44.00 61.472 4.00 2.00 6.472 164 TGTGTCTGTTAAACATGTGTGGTAT 91 25 0 36.00 58.424 7.00 2.00 6.576 165 ATGTGTGGTATTGTGCATATACATGT 105 26 0 34.62 59.420 6.00 6.00 6.580 166 ACATGTGTGGTATTGTGCATATACAT 103 26 0 34.62 59.420 6.00 4.00 6.580 167 GTGTTGTGTATGGATGTAGCTTCT 131 24 0 41.67 57.400 4.00 2.00 6.600 168 TGCATATACATGTGTGTTGTGTATG 118 25 0 36.00 58.378 6.00 5.00 6.622 169 AAACATGTGTGGTATTGTGCATATA 101 25 0 32.00 58.331 6.00 4.00 6.669 170 TAAACATGTGTGGTATTGTGCATAT 100 25 0 32.00 58.331 6.00 4.00 6.669 171 TAGCTTCTTGACTGTGTGTTGTGTG 147 25 0 44.00 61.678 4.00 0.00 6.678 172 TGTAGCTTCTTGACTGTGTGTTGTG 145 25 0 44.00 61.678 4.00 0.00 6.678 173 GTGTGTTGTGTATGGATGTAGCTTCT 129 26 0 42.31 60.727 4.00 2.00 6.727 174 TGTGTCTTGTGGGTGTGTCTGTTA 78 24 0 45.83 62.734 2.00 2.00 6.734 175 ATACATGTGTGTTGTGTATGGATG 123 24 0 37.50 57.241 6.00 0.00 6.759 176 GTGTGTCTGTTAAACATGTGTGGTAT 90 26 0 38.46 59.220 7.00 2.00 6.780 177 ATGGATGTAGCTTCTTGACTGTGTG 140 25 0 44.00 61.791 4.00 0.00 6.791 178 GAACATGTGTGGCATTGTGTATACA 20 25 0 40.00 61.810 8.00 8.00 6.810 179 GTGCATATACATGTGTGTTGTGTATG 117 26 0 38.46 59.190 6.00 5.00 6.810 180 TGTGCATATACATGTGTGTTGTGTAT 116 26 0 34.62 59.190 6.00 3.00 6.810 181 GCATATACATGTGTGTTGTGTATGGA 119 26 0 38.46 60.811 6.00 1.00 6.811 182 CTGTTAAACATGTGTGGTATTGTG 96 24 0 37.50 57.140 7.00 0.00 6.860 183 ATACATGTGTGTTGTGTATGGATGT 123 25 0 36.00 58.124 6.00 0.00 6.876 184 TCTTGTGGGTGTGTCTGTTAAACAT 82 25 0 40.00 61.898 7.00 5.00 6.898 185 AAACATGTGTGGTATTGTGCATATAC 101 26 0 34.62 59.095 6.00 6.00 6.905 186 ATGTGTGTTGTGTATGGATGTAGCTT 127 26 0 38.46 60.948 4.00 2.00 6.948 187 AACATGTGTGGTATTGTGCATATA 102 24 0 33.33 57.002 6.00 4.00 6.998 188 GATGTAGCTTCTTGACTGTGTGTTGT 143 26 0 42.31 61.001 4.00 0.00 7.001 189 CATGTGTGGTATTGTGCATATACATG 104 26 0 38.46 61.147 6.00 6.00 7.147 190 GGTATTGTGCATATACATGTGTGTT 111 25 0 36.00 57.851 6.00 4.00 7.149 191 AACATGTGTGGTATTGTGCATATAC 102 25 0 36.00 57.851 6.00 6.00 7.149 192 GTGTTGTGTATGGATGTAGCTTCTTG 131 26 0 42.31 61.157 4.00 2.00 7.157 193 TGTGTTGTGTATGGATGTAGCTTCTT 130 26 0 38.46 61.157 4.00 4.00 7.157 194 TACATGTGTGTTGTGTATGGATGTA 124 25 0 36.00 57.841 6.00 2.00 7.159 195 TACATGTGTGTTGTGTATGGATGTAG 124 26 0 38.46 58.815 6.00 0.00 7.185 196 GGTGTGTCTGTTAAACATGTGTGGT 89 25 0 44.00 62.217 7.00 0.00 7.217 197 GTGGGTGTGTCTGTTAAACATGTGT 86 25 0 44.00 62.217 7.00 6.00 7.217 198 GTTAAACATGTGTGGTATTGTGCATAT 98 27 0 33.33 60.227 6.00 4.00 7.227 199 CATATACATGTGTGTTGTGTATGGATG 120 27 0 37.04 60.230 6.00 0.00 7.230 200 TGTTAAACATGTGTGGTATTGTGCA 97 25 0 36.00 62.264 7.00 4.00 7.264 201 TGGATGTAGCTTCTTGACTGTGTGT 141 25 0 44.00 62.322 4.00 0.00 7.322 202 TTGTGCATATACATGTGTGTTGTGTAT 115 27 0 33.33 60.389 6.00 3.00 7.389 203 ATTGTGCATATACATGTGTGTTGTGTA 114 27 0 33.33 60.389 6.00 2.00 7.389 204 TATTGTGCATATACATGTGTGTTGTGT 113 27 0 33.33 60.389 6.00 0.00 7.389 205 GTATTGTGCATATACATGTGTGTTGTG 112 27 0 37.04 60.389 6.00 2.00 7.389 206 GTGTCTGTTAAACATGTGTGGTATTGT 92 27 0 37.04 60.396 7.00 0.00 7.396 207 GTGTGTCTGTTAAACATGTGTGGTATT 90 27 0 37.04 60.396 7.00 1.00 7.396 208 TGTTGTGTATAAATGTTGTGTCTTCA 51 26 0 30.77 58.596 4.00 1.00 7.404 209 GTCTTGTGGGTGTGTCTGTTAAACA 81 25 0 44.00 62.427 7.00 7.00 7.427 210 TGTCTTGTGGGTGTGTCTGTTAAAC 80 25 0 44.00 62.427 5.00 5.00 7.427 211 GTGTCTTGTGGGTGTGTCTGTTAAA 79 25 0 44.00 62.427 4.00 3.00 7.427 212 TTAAACATGTGTGGTATTGTGCATATA 99 27 0 29.63 59.560 6.00 4.00 7.440 213 AGCTTCTTGACTGTGTGTTGTGTGT 148 25 0 44.00 62.443 4.00 0.00 7.443 214 AACATGTGTGGTATTGTGCATATACAT 102 27 0 33.33 60.562 6.00 4.00 7.562 215 TAAACATGTGTGGTATTGTGCATATA 100 26 0 30.77 58.395 6.00 4.00 7.605 216 GTGTTGTGTATAAATGTTGTGTCTTCA 50 27 0 33.33 59.358 4.00 1.00 7.642 217 TGTGTTGTGTATAAATGTTGTGTCTTC 49 27 0 33.33 59.358 4.00 0.00 7.642 218 TTGTGGGTGTGTCTGTTAAACATGT 84 25 0 40.00 62.648 7.00 6.00 7.648 219 TATACATGTGTGTTGTGTATGGATG 122 25 0 36.00 57.352 6.00 0.00 7.648 220 CATATACATGTGTGTTGTGTATGGA 120 25 0 36.00 57.352 6.00 0.00 7.648 221 TGCATATACATGTGTGTTGTGTATGG 118 26 0 38.46 61.654 6.00 3.00 7.654 222 TGTGGTATTGTGCATATACATGTGTG 108 26 0 38.46 61.654 6.00 4.00 7.654 223 TGTGTGGTATTGTGCATATACATGTG 106 26 0 38.46 61.654 6.00 4.00 7.654 224 TATGGATGTAGCTTCTTGACTGTGTG 139 26 0 42.31 61.740 4.00 0.00 7.740 225 TGTATGGATGTAGCTTCTTGACTGTG 137 26 0 42.31 61.740 4.00 1.00 7.740 226 TGTGTATGGATGTAGCTTCTTGACTG 135 26 0 42.31 61.740 4.00 1.00 7.740 227 CTTGTGGGTGTGTCTGTTAAACATG 83 25 0 44.00 62.749 7.00 5.00 7.749 228 ATACATGTGTGTTGTGTATGGATGTA 123 26 0 34.62 58.200 6.00 2.00 7.800 229 TATACATGTGTGTTGTGTATGGATGT 122 26 0 34.62 58.200 6.00 0.00 7.800 230 ATGTAGCTTCTTGACTGTGTGTTGTG 144 26 0 42.31 61.844 4.00 0.00 7.844 231 GTCTGTTAAACATGTGTGGTATTGT 94 25 0 36.00 57.143 7.00 0.00 7.857 232 GTGTCTGTTAAACATGTGTGGTATT 92 25 0 36.00 57.143 7.00 1.00 7.857 233 TAAACATGTGTGGTATTGTGCATATAC 100 27 0 33.33 59.133 6.00 6.00 7.867 234 ATACATGTGTGTTGTGTATGGATGTAG 123 27 0 37.04 59.113 6.00 0.00 7.887 235 TATTGTGCATATACATGTGTGTTGT 113 25 0 32.00 57.073 6.00 2.00 7.927 236 GTATTGTGCATATACATGTGTGTTG 112 25 0 36.00 57.073 6.00 4.00 7.927 237 GCATATACATGTGTGTTGTGTATGGAT 119 27 0 37.04 61.010 6.00 2.00 8.010 238 GGTATTGTGCATATACATGTGTGTTGT 111 27 0 37.04 61.034 6.00 2.00 8.034 239 GTGGTATTGTGCATATACATGTGTGTT 109 27 0 37.04 61.034 6.00 4.00 8.034 240 TGTTGTGTATGGATGTAGCTTCTTGA 132 26 0 38.46 62.056 4.00 2.00 8.056 241 GTATTGTGCATATACATGTGTGTTGT 112 26 0 34.62 57.913 6.00 2.00 8.087 242 GTTGTGTATGGATGTAGCTTCTTGACT 133 27 0 40.74 61.126 4.00 1.00 8.126 243 GGTGTGTCTGTTAAACATGTGTGGTA 89 26 0 42.31 62.150 7.00 2.00 8.150 244 ACATGTGTGTTGTGTATGGATGTAGC 125 26 0 42.31 62.163 6.00 2.00 8.163 245 TCTGTTAAACATGTGTGGTATTGTGC 95 26 0 38.46 62.185 7.00 2.00 8.185 246 CATGTGTGTTGTGTATGGATGTAGCT 126 26 0 42.31 62.266 4.00 4.00 8.266 247 ATATACATGTGTGTTGTGTATGGATG 121 26 0 34.62 57.730 6.00 0.00 8.270 248 CATATACATGTGTGTTGTGTATGGAT 120 26 0 34.62 57.730 6.00 2.00 8.270 249 TGTGTTGTGTATAAATGTTGTGTCTT 49 26 0 30.77 57.700 4.00 0.00 8.300 250 TAGCTTCTTGACTGTGTGTTGTGTGT 147 26 0 42.31 62.367 4.00 0.00 8.367 251 GTAGCTTCTTGACTGTGTGTTGTGTG 146 26 0 46.15 62.367 4.00 0.00 8.367 252 TGTAGCTTCTTGACTGTGTGTTGTGT 145 26 0 42.31 62.367 4.00 0.00 8.367 253 TGTGTGTTGTGTATGGATGTAGCTTC 128 26 0 42.31 62.368 4.00 4.00 8.368 254 TGTTAAACATGTGTGGTATTGTGCAT 97 26 0 34.62 62.397 7.00 2.00 8.397 255 AAACATGTGTGGTATTGTGCATATACA 101 27 0 33.33 61.445 6.00 6.00 8.445 256 ATGGATGTAGCTTCTTGACTGTGTGT 140 26 0 42.31 62.454 4.00 0.00 8.454 257 ATATACATGTGTGTTGTGTATGGATGT 121 27 0 33.33 58.526 6.00 0.00 8.474 258 GTCTTGTGGGTGTGTCTGTTAAACAT 81 26 0 42.31 62.553 7.00 5.00 8.553 259 TGTGCATATACATGTGTGTTGTGTATG 116 27 0 37.04 61.688 6.00 5.00 8.688 260 TATACATGTGTGTTGTGTATGGATGTA 122 27 0 33.33 58.271 6.00 2.00 8.729 261 GTGTGTTGTGTATGGATGTAGCTTCTT 129 27 0 40.74 61.811 4.00 4.00 8.811 262 ATGTGTGGTATTGTGCATATACATGTG 105 27 0 37.04 61.814 6.00 4.00 8.814 263 CATGTGTGGTATTGTGCATATACATGT 104 27 0 37.04 61.814 6.00 6.00 8.814 264 ACATGTGTGGTATTGTGCATATACATG 103 27 0 37.04 61.814 6.00 6.00 8.814 265 ATGTGTTGTGTATAAATGTTGTGTCTT 48 27 0 29.63 58.041 4.00 0.00 8.959 266 TGTCTGTTAAACATGTGTGGTATTGTG 93 27 0 37.04 62.089 7.00 0.00 9.089 267 TGTGTCTGTTAAACATGTGTGGTATTG 91 27 0 37.04 62.089 7.00 0.00 9.089 268 TACATGTGTGTTGTGTATGGATGTAGC 124 27 0 40.74 62.100 6.00 2.00 9.100 269 GGTGTGTCTGTTAAACATGTGTGGTAT 89 27 0 40.74 62.286 7.00 2.00 9.286 270 GTGCATATACATGTGTGTTGTGTATGG 117 27 0 40.74 62.312 6.00 3.00 9.312 271 TGTGGTATTGTGCATATACATGTGTGT 108 27 0 37.04 62.312 6.00 6.00 9.312 272 GTGTGGTATTGTGCATATACATGTGTG 107 27 0 40.74 62.312 6.00 4.00 9.312 273 TGTGTGGTATTGTGCATATACATGTGT 106 27 0 37.04 62.312 6.00 6.00 9.312 274 TGTTAAACATGTGTGGTATTGTGCATA 97 27 0 33.33 62.329 7.00 4.00 9.329 275 TATGGATGTAGCTTCTTGACTGTGTGT 139 27 0 40.74 62.383 4.00 0.00 9.383 276 GTATGGATGTAGCTTCTTGACTGTGTG 138 27 0 44.44 62.383 4.00 0.00 9.383 277 TGTATGGATGTAGCTTCTTGACTGTGT 137 27 0 40.74 62.383 4.00 1.00 9.383 278 GTGTATGGATGTAGCTTCTTGACTGTG 136 27 0 44.44 62.383 4.00 1.00 9.383 279 TGTGTATGGATGTAGCTTCTTGACTGT 135 27 0 40.74 62.383 4.00 3.00 9.383 280 ATGTGTGTTGTGTATGGATGTAGCTTC 127 27 0 40.74 62.494 4.00 4.00 9.494 281 ATGTAGCTTCTTGACTGTGTGTTGTGT 144 27 0 40.74 62.495 4.00 0.00 9.495 282 TGTTGTGTATGGATGTAGCTTCTTGAC 132 27 0 40.74 62.685 4.00 1.00 9.685 283 GTGTTGTGTATGGATGTAGCTTCTTGA 131 27 0 40.74 62.685 4.00 2.00 9.685 284 TGGTATTGTGCATATACATGTGTGTTG 110 27 0 37.04 62.716 6.00 4.00 9.716 285 TTGTGTATGGATGTAGCTTCTTGACTG 134 27 0 40.74 62.778 4.00 1.00 9.778 286 GTCTGTTAAACATGTGTGGTATTGTGC 94 27 0 40.74 62.806 7.00 2.00 9.806 287 CATATGTGTTGTGTATAAATGTTGTGT 45 27 0 29.63 57.112 6.00 0.00 9.888 288 ACATATGTGTTGTGTATAAATGTTGTG 44 27 0 29.63 57.112 8.00 0.00 9.888 289 CACATATGTGTTGTGTATAAATGTTGT 43 27 0 29.63 57.112 10.00 2.00 9.888 290 ACACATATGTGTTGTGTATAAATGTTG 42 27 0 29.63 57.112 12.00 0.00 9.888 291 ACATGTGTGTTGTGTATGGATGTAGCT 125 27 0 40.74 62.902 6.00 4.00 9.902 primer3-2.3.6/test/primer_list_last/case_problem_test.rev0000664000077300007730000002237112231156244022625 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 tctacctcctcaatggtggg 1918 20 0 55.00 59.920 4.00 2.00 0.080 1 atctacctcctcaatggtggg 1919 21 0 52.38 60.197 4.00 2.00 1.197 2 ctgacccagaccctccct 1896 18 0 66.67 60.038 3.00 0.00 2.038 3 ctgacccagaccctccctt 1896 19 0 63.16 61.450 3.00 0.00 2.450 4 tgacccagaccctccctt 1895 18 0 61.11 60.457 2.00 0.00 2.457 5 gacccagaccctcccttg 1894 18 0 66.67 60.457 2.00 0.00 2.457 6 ctacctcctcaatggtggg 1917 19 0 57.89 57.927 4.00 2.00 3.073 7 ctgacccagaccctcccttg 1896 20 0 65.00 64.268 3.00 0.00 4.268 8 acccagaccctcccttgg 1893 18 0 66.67 62.292 3.00 2.00 4.292 9 tacctcctcaatggtggggc 1916 20 0 60.00 64.378 4.00 2.00 4.378 10 tgacccagaccctcccttg 1895 19 0 63.16 63.451 2.00 0.00 4.451 11 cctgacccagaccctccct 1897 19 0 68.42 63.784 3.00 0.00 4.784 12 cctgacccagaccctccctt 1897 20 0 65.00 64.906 3.00 0.00 4.906 13 cctgacccagaccctccc 1897 18 0 72.22 62.920 3.00 0.00 4.920 14 ggcctgacccagaccctc 1899 18 0 72.22 63.082 5.00 1.00 5.082 15 gcctgacccagaccctcc 1898 18 0 72.22 63.082 3.00 0.00 5.082 16 ctcaatggtggggcctga 1910 18 0 61.11 63.108 4.00 2.00 5.108 17 gacccagaccctcccttgg 1894 19 0 68.42 64.147 3.00 2.00 5.147 18 tacctcctcaatggtggg 1916 18 0 55.56 56.726 4.00 2.00 5.274 19 acctcctcaatggtggggc 1915 19 0 63.16 64.541 4.00 2.00 5.541 20 ctcctcaatggtggggcct 1913 19 0 63.16 64.641 4.00 2.00 5.641 21 tcctcaatggtggggcct 1912 18 0 61.11 63.841 4.00 2.00 5.841 22 ctacctcctcaatggtggggc 1917 21 0 61.90 65.094 4.00 2.00 6.094 23 tgacccagaccctcccttgg 1895 20 0 65.00 66.847 3.00 2.00 6.847 24 cctcaatggtggggcctg 1911 18 0 66.67 64.865 4.00 1.00 6.865 25 gcctgacccagaccctccct 1898 20 0 70.00 67.224 3.00 0.00 7.224 26 ctcctcaatggtggggcctg 1913 20 0 65.00 67.289 4.00 1.00 7.289 27 ggcctgacccagaccctcc 1899 19 0 73.68 66.583 5.00 0.00 7.583 28 gcctgacccagaccctccc 1898 19 0 73.68 66.583 3.00 0.00 7.583 29 tcctcaatggtggggcctg 1912 19 0 63.16 66.645 4.00 1.00 7.645 30 cctcaatggtggggcctga 1911 19 0 63.16 66.645 4.00 2.00 7.645 31 cctcctcaatggtggggcct 1914 20 0 65.00 67.819 4.00 2.00 7.819 32 tcctcaatggtggggcctga 1912 20 0 60.00 68.314 4.00 2.00 8.314 33 cctgacccagaccctcccttg 1897 21 0 66.67 67.461 3.00 0.00 8.461 34 ctgacccagaccctcccttgg 1896 21 0 66.67 67.461 3.00 2.00 8.461 35 tctacctcctcaatggtggggc 1918 22 0 59.09 66.659 4.00 2.00 8.659 36 atctacctcctcaatggtg 1919 19 0 47.37 52.239 3.00 2.00 8.761 37 ggtggggcctgacccaga 1904 18 0 72.22 66.913 6.00 2.00 8.913 38 agaccctcccttgggaccctg 1889 21 0 66.67 67.969 6.00 1.00 8.969 39 gcctgacccagaccctccctt 1898 21 0 66.67 68.106 3.00 0.00 9.106 40 acctcctcaatggtggggcct 1915 21 0 61.90 68.303 4.00 2.00 9.303 41 atggtggggcctgacccaga 1906 20 0 65.00 69.400 6.00 2.00 9.400 42 atctacctcctcaatggtggggc 1919 23 0 56.52 66.584 4.00 2.00 9.584 43 gggcctgacccagaccctcc 1900 20 0 75.00 69.671 7.00 1.00 9.671 44 ggcctgacccagaccctccc 1899 20 0 75.00 69.671 5.00 0.00 9.671 45 ctcctcaatggtggggcctga 1913 21 0 61.90 68.862 4.00 2.00 9.862 46 tacctcctcaatggtggggcct 1916 22 0 59.09 68.025 4.00 2.00 10.025 47 tctacctcctcaatggtg 1918 18 0 50.00 51.404 3.00 2.00 10.596 48 tggtggggcctgacccaga 1905 19 0 68.42 69.649 6.00 2.00 10.649 49 ggcctgacccagaccctccct 1899 21 0 71.43 70.138 5.00 0.00 11.138 50 aatggtggggcctgacccaga 1907 21 0 61.90 70.180 6.00 2.00 11.180 51 cctcctcaatggtggggcctg 1914 21 0 66.67 70.229 4.00 1.00 11.229 52 agaccctcccttgggaccctga 1889 22 0 63.64 69.431 6.00 1.00 11.431 53 ctacctcctcaatggtggggcct 1917 23 0 60.87 68.525 4.00 2.00 11.525 54 cctgacccagaccctcccttgg 1897 22 0 68.18 70.301 3.00 2.00 12.301 55 cagaccctcccttgggaccctg 1890 22 0 68.18 70.301 6.00 1.00 12.301 56 gcctgacccagaccctcccttg 1898 22 0 68.18 70.433 3.00 0.00 12.433 57 acctcctcaatggtggggcctg 1915 22 0 63.64 70.613 4.00 1.00 12.613 58 ggcctgacccagaccctccctt 1899 22 0 68.18 70.831 5.00 0.00 12.831 59 atctacctcctcaatggt 1919 18 0 44.44 48.744 3.00 0.00 13.256 60 tacctcctcaatggtggggcctg 1916 23 0 60.87 70.264 4.00 1.00 13.264 61 gggcctgacccagaccctccc 1900 21 0 76.19 72.413 7.00 0.00 13.413 62 cctcctcaatggtggggcctga 1914 22 0 63.64 71.632 4.00 2.00 13.632 63 tctacctcctcaatggtggggcct 1918 24 0 58.33 69.859 4.00 2.00 13.859 64 caatggtggggcctgacccaga 1908 22 0 63.64 72.477 6.00 2.00 14.477 65 atctacctcctcaatggtggggcct 1919 25 0 56.00 69.650 4.00 2.00 14.650 66 cagaccctcccttgggaccctga 1890 23 0 65.22 71.660 6.00 1.00 14.660 67 ctacctcctcaatggtggggcctg 1917 24 0 62.50 70.663 4.00 1.00 14.663 68 gggcctgacccagaccctccct 1900 22 0 72.73 72.741 7.00 0.00 14.741 69 acctcctcaatggtggggcctga 1915 23 0 60.87 71.957 4.00 2.00 14.957 70 tacctcctcaatggtggggcctga 1916 24 0 58.33 71.573 4.00 2.00 15.573 71 ccagaccctcccttgggaccctg 1891 23 0 69.57 72.843 6.00 1.00 15.843 72 ggcctgacccagaccctcccttg 1899 23 0 69.57 72.968 5.00 0.00 15.968 73 gcctgacccagaccctcccttgg 1898 23 0 69.57 72.968 3.00 2.00 15.968 74 gggcctgacccagaccctccctt 1900 23 0 69.57 73.279 7.00 0.00 16.279 75 agaccctcccttgggaccctgagc 1889 24 0 66.67 72.459 6.00 2.00 16.459 76 tcaatggtggggcctgacccaga 1909 23 0 60.87 73.791 6.00 2.00 16.791 77 ggggcctgacccagaccctccc 1901 22 0 77.27 74.866 7.00 1.00 16.866 78 tctacctcctcaatggtggggcctg 1918 25 0 60.00 71.911 4.00 1.00 16.911 79 ctacctcctcaatggtggggcctga 1917 25 0 60.00 71.911 4.00 2.00 16.911 80 atctacctcctcaatggtggggcctg 1919 26 0 57.69 71.619 4.00 1.00 17.619 81 ctcaatggtggggcctgacccaga 1910 24 0 62.50 74.055 6.00 2.00 18.055 82 ccagaccctcccttgggaccctga 1891 24 0 66.67 74.067 6.00 1.00 18.067 83 ggggcctgacccagaccctccct 1901 23 0 73.91 75.080 7.00 0.00 18.080 84 tctacctcctcaatggtggggcctga 1918 26 0 57.69 73.097 4.00 2.00 19.097 85 cccagaccctcccttgggaccctg 1892 24 0 70.83 75.132 6.00 1.00 19.132 86 gggcctgacccagaccctcccttg 1900 24 0 70.83 75.250 7.00 0.00 19.250 87 ggcctgacccagaccctcccttgg 1899 24 0 70.83 75.250 5.00 2.00 19.250 88 cagaccctcccttgggaccctgagc 1890 25 0 68.00 74.421 6.00 2.00 19.421 89 ggggcctgacccagaccctccctt 1901 24 0 70.83 75.489 7.00 0.00 19.489 90 agaccctcccttgggaccctgagcc 1889 25 0 68.00 74.677 6.00 1.00 19.677 91 atctacctcctcaatggtggggcctga 1919 27 0 55.56 72.762 4.00 2.00 19.762 92 tggggcctgacccagaccctccc 1902 23 0 73.91 76.866 7.00 1.00 19.866 93 acccagaccctcccttgggaccctg 1893 25 0 68.00 75.315 6.00 1.00 20.315 94 tggggcctgacccagaccctccct 1902 24 0 70.83 76.998 7.00 0.00 20.998 95 cccagaccctcccttgggaccctga 1892 25 0 68.00 76.240 6.00 1.00 21.240 96 cctcaatggtggggcctgacccaga 1911 25 0 64.00 76.273 6.00 2.00 21.273 97 gtggggcctgacccagaccctccct 1903 25 0 72.00 77.122 7.00 1.00 22.122 98 tggggcctgacccagaccctccctt 1902 25 0 68.00 77.315 7.00 0.00 22.315 99 ggggcctgacccagaccctcccttg 1901 25 0 72.00 77.315 7.00 0.00 22.315 100 gggcctgacccagaccctcccttgg 1900 25 0 72.00 77.315 7.00 2.00 22.315 101 gacccagaccctcccttgggaccctg 1894 26 0 69.23 76.385 6.00 1.00 22.385 102 acccagaccctcccttgggaccctga 1893 26 0 65.38 76.385 6.00 1.00 22.385 103 ccagaccctcccttgggaccctgagc 1891 26 0 69.23 76.497 6.00 2.00 22.497 104 cagaccctcccttgggaccctgagcc 1890 26 0 69.23 76.497 6.00 1.00 22.497 105 tcctcaatggtggggcctgacccaga 1912 26 0 61.54 77.349 6.00 2.00 23.349 106 gtggggcctgacccagaccctccctt 1903 26 0 69.23 77.425 7.00 0.00 23.425 107 gacccagaccctcccttgggaccctga 1894 27 0 66.67 77.405 6.00 1.00 24.405 108 ctcctcaatggtggggcctgacccaga 1913 27 0 62.96 77.454 6.00 2.00 24.454 109 tggggcctgacccagaccctcccttg 1902 26 0 69.23 79.060 7.00 0.00 25.060 110 tgacccagaccctcccttgggaccctg 1895 27 0 66.67 78.138 6.00 1.00 25.138 111 ggggcctgacccagaccctcccttgg 1901 26 0 73.08 79.194 7.00 2.00 25.194 112 cccagaccctcccttgggaccctgagc 1892 27 0 70.37 78.388 6.00 2.00 25.388 113 ccagaccctcccttgggaccctgagcc 1891 27 0 70.37 78.388 6.00 1.00 25.388 114 gtggggcctgacccagaccctcccttg 1903 27 0 70.37 79.116 7.00 0.00 26.116 115 tggggcctgacccagaccctcccttgg 1902 27 0 70.37 80.820 7.00 2.00 27.820 primer3-2.3.6/test/primer_list_last/long_seq.for0000664000077300007730000022057012231156244020735 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 gcgatcgatgctagctagct 372 20 0 55.00 59.880 10.00 8.00 0.120 1 gatcgatgcatgcatgctac 445 20 0 50.00 60.222 10.00 8.00 0.222 2 gatgctagctaggcgatgct 408 20 0 55.00 59.742 8.00 3.00 0.258 3 cggcgatctagctagctgac 581 20 0 60.00 60.278 8.00 6.00 0.278 4 cgtagcggcgatctagctag 576 20 0 60.00 60.285 8.00 8.00 0.285 5 tagcggcgatctagctagct 578 20 0 55.00 59.523 8.00 8.00 0.477 6 atcgatgtgcggctagctag 85 20 0 55.00 60.531 8.00 8.00 0.531 7 tatcatcgatcgatgcatgc 438 20 0 45.00 60.574 12.00 8.00 0.574 8 gtagcggcgatctagctagc 577 20 0 60.00 59.393 8.00 6.00 0.607 9 gatcgatgctagctaggcga 404 20 0 55.00 60.615 8.00 4.00 0.615 10 tcgatcgatgctagctaggc 402 20 0 55.00 60.615 8.00 6.00 0.615 11 gctagctgactgatacgcga 467 20 0 55.00 59.351 6.00 2.00 0.649 12 atcgatgcatgcatgctact 446 20 0 45.00 59.285 10.00 7.00 0.715 13 atcgatgctacatcgatcga 314 20 0 45.00 59.209 12.00 8.00 0.791 14 atcgatgctagctaggcgat 405 20 0 50.00 59.074 8.00 6.00 0.926 15 tcgatgcatgcatgctacta 447 20 0 45.00 58.989 10.00 8.00 1.011 16 tcgatgcatgcatgctactag 447 21 0 47.62 60.014 10.00 7.00 1.014 17 gctagctgatcgatcgatgc 393 20 0 55.00 61.025 10.00 8.00 1.025 18 gactgatcgatcgatgctagc 115 21 0 52.38 59.961 10.00 6.00 1.039 19 ctgactgatcgatcgatgct 113 20 0 50.00 58.936 10.00 6.00 1.064 20 agctgactgatcgatcgatg 111 20 0 50.00 58.936 10.00 8.00 1.064 21 gctagctagctgatcgatcga 389 21 0 52.38 59.866 10.00 8.00 1.134 22 gctagctagctgatcgatcga 69 21 0 52.38 59.866 10.00 8.00 1.134 23 tcgtagcggcgatctagcta 575 20 0 55.00 61.150 6.00 6.00 1.150 24 cgcgatcgatgctagctag 371 19 0 57.89 59.848 8.00 8.00 1.152 25 gctactatcatctctgcgcg 355 20 0 55.00 58.801 4.00 4.00 1.199 26 ctagctgatcgatcgtagcg 563 20 0 55.00 58.799 8.00 4.00 1.201 27 agctactatcatctctgcgcg 354 21 0 52.38 59.789 4.00 4.00 1.211 28 gatgctagctaggcgatgcta 408 21 0 52.38 59.761 8.00 5.00 1.239 29 agctgactagctgatcgatcg 594 21 0 52.38 59.755 8.00 6.00 1.245 30 agctagctgactgatcgatcg 107 21 0 52.38 59.755 8.00 6.00 1.245 31 tcgatgtgcggctagctag 86 19 0 57.89 60.272 8.00 8.00 1.272 32 gctgactagctgatcgatcg 595 20 0 55.00 58.726 8.00 6.00 1.274 33 gctagctgactgatcgatcg 108 20 0 55.00 58.726 8.00 6.00 1.274 34 cgatgctagctaggcgatg 407 19 0 57.89 59.703 8.00 4.00 1.297 35 gtagcggcgatctagctagct 577 21 0 57.14 60.297 8.00 8.00 1.297 36 agctagctgactgatacgcga 466 21 0 52.38 60.328 8.00 2.00 1.328 37 gactgatacgcgatgctagct 474 21 0 52.38 59.657 6.00 4.00 1.343 38 gctagctgactgatacgcgat 467 21 0 52.38 59.657 6.00 4.00 1.343 39 gactgatacgcgatgctagc 474 20 0 55.00 58.655 6.00 6.00 1.345 40 gctgactagctgatcgatcgt 595 21 0 52.38 59.618 8.00 4.00 1.382 41 atcgtagcggcgatctagct 574 20 0 55.00 61.387 6.00 4.00 1.387 42 tgctagctaggcgatgctag 410 20 0 55.00 58.609 8.00 7.00 1.391 43 tagctgatcgatcgtagcgg 564 20 0 55.00 61.420 8.00 4.00 1.420 44 gctaggcgatgctagctagct 415 21 0 57.14 60.424 10.00 8.00 1.424 45 gctagctaggcgatgctagct 411 21 0 57.14 60.424 9.00 8.00 1.424 46 gatcgatgtgcggctagcta 84 20 0 55.00 61.432 6.00 6.00 1.432 47 atcgatgtgcggctagcta 85 19 0 52.63 59.563 6.00 6.00 1.437 48 atcgtagcggcgatctagc 574 19 0 57.89 60.493 6.00 2.00 1.493 49 agcggcgatctagctagct 579 19 0 57.89 59.495 8.00 8.00 1.505 50 gctgactgatacgcgatgct 471 20 0 55.00 61.520 4.00 0.00 1.520 51 agctgactgatacgcgatgc 470 20 0 55.00 61.520 4.00 2.00 1.520 52 agctagctgactgatacgcg 466 20 0 55.00 58.460 8.00 4.00 1.540 53 gctgactgatacgcgatgc 471 19 0 57.89 60.553 4.00 2.00 1.553 54 tcgatgctagctaggcgatg 406 20 0 55.00 61.554 8.00 4.00 1.554 55 cggcgatctagctagctga 581 19 0 57.89 59.435 8.00 8.00 1.565 56 atcatcgatcgatgcatgc 439 19 0 47.37 60.587 12.00 6.00 1.587 57 agctgatcatcatcgatgct 514 20 0 45.00 58.361 8.00 8.00 1.639 58 cgtagcggcgatctagcta 576 19 0 57.89 59.339 6.00 6.00 1.661 59 gctagctgactgatcgatcga 108 21 0 52.38 60.661 8.00 8.00 1.661 60 gctactatcatctctgcgcga 355 21 0 52.38 60.662 4.00 4.00 1.662 61 atcgatgcatgcatgctacta 446 21 0 42.86 59.324 10.00 8.00 1.676 62 gatcgatgcatgcatgcta 445 19 0 47.37 59.309 10.00 8.00 1.691 63 tcggattagctagctgatgct 6 21 0 47.62 59.242 10.00 8.00 1.758 64 agcatcggattagctagctga 2 21 0 47.62 59.242 8.00 8.00 1.758 65 gcatcggattagctagctga 3 20 0 50.00 58.241 8.00 8.00 1.759 66 agctagctgatcatcgatgct 189 21 0 47.62 59.216 8.00 8.00 1.784 67 tgctagctagctgatcgatcg 557 21 0 52.38 60.787 10.00 6.00 1.787 68 cgatgctagctagctgatcga 554 21 0 52.38 60.787 10.00 7.00 1.787 69 tgactgatcgatcatcatgct 266 21 0 42.86 59.212 12.00 5.00 1.788 70 ctgactgatcgatcatcatgc 265 21 0 47.62 59.212 12.00 6.00 1.788 71 ctgatcgatcgatgctagct 398 20 0 50.00 58.173 10.00 4.00 1.827 72 agctgatcgatcgatgctag 396 20 0 50.00 58.173 10.00 8.00 1.827 73 ctagctgatcgatcgatgct 394 20 0 50.00 58.173 10.00 8.00 1.827 74 agctagctgatcgatcgatg 392 20 0 50.00 58.173 10.00 8.00 1.827 75 ctgatcgatcgatgctagct 117 20 0 50.00 58.173 10.00 4.00 1.827 76 agctagctgatcgatcgatg 72 20 0 50.00 58.173 10.00 8.00 1.827 77 gctagctgatcatcgatgct 190 20 0 50.00 58.173 8.00 8.00 1.827 78 agctagctgatcatcgatgc 189 20 0 50.00 58.173 8.00 6.00 1.827 79 gatcgatgctagctaggcgat 404 21 0 52.38 60.845 8.00 6.00 1.845 80 atcgatcgatgctagctaggc 401 21 0 52.38 60.845 10.00 6.00 1.845 81 gctagctgatcgatcgatgtg 73 21 0 52.38 60.920 10.00 8.00 1.920 82 gctgactgatcgatcgatgc 112 20 0 55.00 61.922 10.00 8.00 1.922 83 cgatgcatgcatgctactag 448 20 0 50.00 58.074 10.00 6.00 1.926 84 actgatcgatcgatgctagct 116 21 0 47.62 59.073 10.00 6.00 1.927 85 agctagctgatcgatcgatgt 72 21 0 47.62 59.073 10.00 6.00 1.927 86 ctgatgctagtgatgcatgct 19 21 0 47.62 59.068 6.00 4.00 1.932 87 agctgatgctagtgatgcatg 17 21 0 47.62 59.068 6.00 4.00 1.932 88 tgactgatcgatcatcatgc 266 20 0 45.00 58.063 12.00 6.00 1.937 89 gatcgatcgatgctagctagg 400 21 0 52.38 59.060 10.00 6.00 1.940 90 ctatcatctctgcgcgatcg 359 20 0 55.00 61.980 6.00 6.00 1.980 91 actatcatctctgcgcgatc 358 20 0 50.00 58.018 4.00 4.00 1.982 92 actgatcgatcgatgctagc 116 20 0 50.00 58.018 10.00 6.00 1.982 93 gctagctgatcgatcgatgt 73 20 0 50.00 58.018 10.00 6.00 1.982 94 agctagctagctgatcgatcg 388 21 0 52.38 59.013 12.00 6.00 1.987 95 agctagctagctgatcgatcg 68 21 0 52.38 59.013 12.00 6.00 1.987 96 ctgactgatcgatcgatgctag 113 22 0 50.00 60.000 10.00 8.00 2.000 97 ctagctgactgatcgatcgatg 109 22 0 50.00 60.000 10.00 8.00 2.000 98 gctagctagctgatcgatcg 558 20 0 55.00 57.998 10.00 6.00 2.002 99 gctagctagctgatcgatcg 389 20 0 55.00 57.998 10.00 6.00 2.002 100 gctagctagctgatcgatcg 69 20 0 55.00 57.998 10.00 6.00 2.002 101 tgactgatcgatcgatgctag 114 21 0 47.62 58.990 10.00 8.00 2.010 102 ctgactgatcgatcgatgcta 113 21 0 47.62 58.990 10.00 6.00 2.010 103 tagctgactgatcgatcgatg 110 21 0 47.62 58.990 10.00 8.00 2.010 104 aaagcatcggattagctagctg 0 22 0 45.45 60.030 8.00 6.00 2.030 105 tgatgctagtgatgcatgct 20 20 0 45.00 57.966 6.00 4.00 2.034 106 ctgatgctagtgatgcatgc 19 20 0 50.00 57.966 6.00 6.00 2.034 107 gctgatgctagtgatgcatg 18 20 0 50.00 57.966 6.00 4.00 2.034 108 tatcatctctgcgcgatcg 360 19 0 52.63 61.047 6.00 6.00 2.047 109 atgctagctaggcgatgcta 409 20 0 50.00 57.952 8.00 5.00 2.048 110 gatacgcgatgctagctagct 478 21 0 52.38 58.940 10.00 8.00 2.060 111 atgctagctaggcgatgctag 409 21 0 52.38 58.937 8.00 7.00 2.063 112 tcatcgatgctagctagctga 550 21 0 47.62 58.934 10.00 8.00 2.066 113 tcgatgcatgcatgctact 447 19 0 47.37 58.933 10.00 6.00 2.067 114 gctactgatcgatgctacatcg 307 22 0 50.00 59.895 8.00 8.00 2.105 115 ctgatcgatcgatgtgcg 78 18 0 55.56 59.889 10.00 4.00 2.111 116 gctagctagctgatcgatcgt 558 21 0 52.38 58.876 10.00 4.00 2.124 117 tgactgatcgatcgatgcta 114 20 0 45.00 57.875 10.00 6.00 2.125 118 tcgatgctacatcgatcga 315 19 0 47.37 58.849 10.00 8.00 2.151 119 cggcgatctagctagctgact 581 21 0 57.14 61.177 8.00 6.00 2.177 120 agcggcgatctagctagctg 579 20 0 60.00 62.179 8.00 6.00 2.179 121 tcgtagcggcgatctagct 575 19 0 57.89 61.184 4.00 4.00 2.184 122 gatcgatgcatgcatgctact 445 21 0 47.62 61.188 10.00 6.00 2.188 123 tagctactatcatctctgcgcg 353 22 0 50.00 59.806 6.00 4.00 2.194 124 actgatacgcgatgctagct 475 20 0 50.00 57.781 6.00 4.00 2.219 125 aagcatcggattagctagctg 1 21 0 47.62 58.779 8.00 6.00 2.221 126 tagctgactagctgatcgatcg 593 22 0 50.00 59.775 8.00 6.00 2.225 127 tagctagctgactgatcgatcg 106 22 0 50.00 59.775 8.00 6.00 2.225 128 tcgtagcggcgatctagc 575 18 0 61.11 60.228 4.00 2.00 2.228 129 ctgactgatcgatcatcatgct 265 22 0 45.45 60.249 12.00 5.00 2.249 130 gatgctagctaggcgatgc 408 19 0 57.89 58.740 8.00 4.00 2.260 131 tcgatgctagctaggcgat 406 19 0 52.63 58.738 8.00 6.00 2.262 132 atcgatgctagctaggcga 405 19 0 52.63 58.738 8.00 4.00 2.262 133 gatcgatgctagctaggcg 404 19 0 57.89 58.738 8.00 4.00 2.262 134 cgatcgatgctagctaggc 403 19 0 57.89 58.738 8.00 6.00 2.262 135 gatcgtagcggcgatctagc 573 20 0 60.00 62.271 8.00 2.00 2.271 136 atcgatgcatgcatgctactag 446 22 0 45.45 60.280 10.00 6.00 2.280 137 ctgactgatacgcgatgctag 472 21 0 52.38 58.715 4.00 4.00 2.285 138 ctagctgactgatacgcgatg 468 21 0 52.38 58.715 4.00 2.00 2.285 139 tcatcgatcgatgcatgc 440 18 0 50.00 60.297 12.00 6.00 2.297 140 gcggcgatctagctagctg 580 19 0 63.16 61.320 8.00 6.00 2.320 141 gactgatacgcgatgctagcta 474 22 0 50.00 59.680 6.00 6.00 2.320 142 tagctagctgactgatacgcga 465 22 0 50.00 60.326 8.00 3.00 2.326 143 atcgtagcggcgatctagcta 574 21 0 52.38 61.348 6.00 6.00 2.348 144 gctgactagctgatcgatcgta 595 22 0 50.00 59.643 8.00 6.00 2.357 145 tgactgatacgcgatgctag 473 20 0 50.00 57.632 4.00 4.00 2.368 146 ctgactgatacgcgatgcta 472 20 0 50.00 57.632 4.00 2.00 2.368 147 tagctgactgatacgcgatg 469 20 0 50.00 57.632 4.00 2.00 2.368 148 gatcgtagcggcgatctag 573 19 0 57.89 58.588 8.00 4.00 2.412 149 tagcggcgatctagctagc 578 19 0 57.89 58.547 8.00 6.00 2.453 150 agctgatcgatcgtagcgg 565 19 0 57.89 61.465 8.00 4.00 2.465 151 atcgatgtgcggctagct 85 18 0 55.56 59.533 6.00 4.00 2.467 152 atcggattagctagctgatgct 5 22 0 45.45 59.532 10.00 8.00 2.468 153 tagctagctgactgatacgcg 465 21 0 52.38 58.528 8.00 4.00 2.472 154 tgactgatacgcgatgctagc 473 21 0 52.38 61.473 6.00 6.00 2.473 155 gctgactgatacgcgatgcta 471 21 0 52.38 61.473 4.00 2.00 2.473 156 tagctgactgatacgcgatgc 469 21 0 52.38 61.473 4.00 2.00 2.473 157 gatcgatgtgcggctagct 84 19 0 57.89 61.479 6.00 4.00 2.479 158 ctgatcgatcgtagcggc 567 18 0 61.11 60.490 8.00 3.00 2.490 159 gctgatcgatcgtagcgg 566 18 0 61.11 60.490 8.00 5.00 2.490 160 aagcatcggattagctagctga 1 22 0 45.45 60.497 8.00 8.00 2.497 161 gatcgatgtgcggctagc 84 18 0 61.11 60.500 6.00 6.00 2.500 162 ctagctagctgactgatacgcg 464 22 0 54.55 59.491 8.00 4.00 2.509 163 atcgatcatcgatgctagct 545 20 0 45.00 57.480 11.00 8.00 2.520 164 ctgatcatcatcgatgctagct 516 22 0 45.45 59.462 8.00 6.00 2.538 165 agctgatcatcatcgatgctag 514 22 0 45.45 59.462 8.00 6.00 2.538 166 ctagctgatcatcatcgatgct 512 22 0 45.45 59.462 8.00 8.00 2.538 167 agctagctgatcatcatcgatg 510 22 0 45.45 59.462 8.00 8.00 2.538 168 ctgatcgatcatcatgctagct 269 22 0 45.45 59.462 10.00 5.00 2.538 169 tgatcatcatcgatgctagct 517 21 0 42.86 58.438 8.00 6.00 2.562 170 ctgatcatcatcgatgctagc 516 21 0 47.62 58.438 8.00 6.00 2.562 171 gctgatcatcatcgatgctag 515 21 0 47.62 58.438 8.00 6.00 2.562 172 agctgatcatcatcgatgcta 514 21 0 42.86 58.438 8.00 6.00 2.562 173 tagctgatcatcatcgatgct 513 21 0 42.86 58.438 8.00 8.00 2.562 174 ctagctgatcatcatcgatgc 512 21 0 47.62 58.438 8.00 6.00 2.562 175 gctagctgatcatcatcgatg 511 21 0 47.62 58.438 8.00 8.00 2.562 176 tgatcgatcatcatgctagct 270 21 0 42.86 58.438 10.00 5.00 2.562 177 ctgatcgatcatcatgctagc 269 21 0 47.62 58.438 10.00 6.00 2.562 178 agctgactagctgatcgatcgt 594 22 0 50.00 60.566 8.00 4.00 2.566 179 agctagctgactgatacgcgat 466 22 0 50.00 60.570 8.00 4.00 2.570 180 gatgtgcggctagctagct 88 19 0 57.89 58.394 10.00 8.00 2.606 181 cggattagctagctgatgct 7 20 0 50.00 57.392 10.00 8.00 2.608 182 agcatcggattagctagctg 2 20 0 50.00 57.392 8.00 6.00 2.608 183 catcgatcgatgctagtatgc 324 21 0 47.62 58.376 12.00 4.00 2.624 184 catcgatcgatgctagtatgct 324 22 0 45.45 59.370 12.00 4.00 2.630 185 atcgatcgatgcatgcatg 442 19 0 47.37 61.636 10.00 8.00 2.636 186 gatgctagctaggcgatgctag 408 22 0 54.55 60.642 8.00 7.00 2.642 187 actgatcgatcatcatgctagc 268 22 0 45.45 59.325 10.00 6.00 2.675 188 tgatcatcatcgatgctagc 517 20 0 45.00 57.307 8.00 6.00 2.693 189 gctgatcatcatcgatgcta 515 20 0 45.00 57.307 8.00 6.00 2.693 190 tagctgatcatcatcgatgc 513 20 0 45.00 57.307 8.00 6.00 2.693 191 tgatcgatcatcatgctagc 270 20 0 45.00 57.307 10.00 6.00 2.693 192 cgtagcggcgatctagct 576 18 0 61.11 59.301 4.00 4.00 2.699 193 tgcatgcatgctactagctagc 451 22 0 50.00 60.706 10.00 8.00 2.706 194 gctagctgatgctagtgatgca 14 22 0 50.00 60.707 7.00 4.00 2.707 195 gatcgatgcatgcatgct 445 18 0 50.00 59.265 10.00 8.00 2.735 196 atcgatgctagctaggcgatg 405 21 0 52.38 61.735 8.00 5.00 2.735 197 catcgatgctagctagctgatc 551 22 0 50.00 59.256 10.00 8.00 2.744 198 atcatcgatgctagctagctga 549 22 0 45.45 59.256 10.00 8.00 2.744 199 gatcatcgatgctagctagctg 548 22 0 50.00 59.256 10.00 6.00 2.744 200 agctagctgatcatcgatgcta 189 22 0 45.45 59.256 8.00 7.00 2.744 201 tagctagctgatcatcgatgct 188 22 0 45.45 59.256 8.00 8.00 2.744 202 ctagctagctgatcatcgatgc 187 22 0 50.00 59.256 8.00 6.00 2.744 203 gctagctagctgatcatcgatg 186 22 0 50.00 59.256 10.00 8.00 2.744 204 tgactgatcgatcatcatgcta 266 22 0 40.91 59.256 12.00 5.00 2.744 205 gctagctgatcatcgatgcta 190 21 0 47.62 58.255 8.00 7.00 2.745 206 tagctagctgatcatcgatgc 188 21 0 47.62 58.255 8.00 6.00 2.745 207 tcgatcatcgatgctagctag 546 21 0 47.62 58.254 9.00 8.00 2.746 208 tgatcgatcgatgctagctag 399 21 0 47.62 58.254 10.00 8.00 2.746 209 ctgatcgatcgatgctagcta 398 21 0 47.62 58.254 10.00 6.00 2.746 210 tagctgatcgatcgatgctag 395 21 0 47.62 58.254 10.00 8.00 2.746 211 tagctagctgatcgatcgatg 391 21 0 47.62 58.254 10.00 8.00 2.746 212 tgatcgatcgatgctagctag 118 21 0 47.62 58.254 10.00 8.00 2.746 213 ctgatcgatcgatgctagcta 117 21 0 47.62 58.254 10.00 6.00 2.746 214 tagctagctgatcgatcgatg 71 21 0 47.62 58.254 10.00 8.00 2.746 215 ctgatcgatcgatgctagctag 398 22 0 50.00 59.252 10.00 8.00 2.748 216 ctagctagctgatcgatcgatg 390 22 0 50.00 59.252 10.00 8.00 2.748 217 ctgatcgatcgatgctagctag 117 22 0 50.00 59.252 10.00 8.00 2.748 218 ctagctagctgatcgatcgatg 70 22 0 50.00 59.252 10.00 8.00 2.748 219 acatcgatcgatgctagtatgc 323 22 0 45.45 59.236 12.00 4.00 2.764 220 tcgatgtgcggctagcta 86 18 0 55.56 59.232 6.00 6.00 2.768 221 agctagctagctgatcgatcga 388 22 0 50.00 60.769 12.00 8.00 2.769 222 agctagctagctgatcgatcga 68 22 0 50.00 60.769 12.00 8.00 2.769 223 gatgcatgcatgctactagct 449 21 0 47.62 58.187 10.00 5.00 2.813 224 atcgatgcatgcatgctac 446 19 0 47.37 58.187 10.00 8.00 2.813 225 atcgatcgatgctagctagg 401 20 0 50.00 57.180 10.00 6.00 2.820 226 tcgatcatcgatgctagcta 546 20 0 45.00 57.158 9.00 6.00 2.842 227 tgatcgatcgatgctagcta 399 20 0 45.00 57.158 10.00 6.00 2.842 228 tgatcgatcgatgctagcta 118 20 0 45.00 57.158 10.00 6.00 2.842 229 cgatgcatgcatgctactagc 448 21 0 52.38 61.851 10.00 7.00 2.851 230 atacgcgatgctagctagct 479 20 0 50.00 57.129 10.00 8.00 2.871 231 gctgatcatcgatgctactagc 194 22 0 50.00 59.121 8.00 8.00 2.879 232 gctagctgatcatcgatgctac 190 22 0 50.00 59.121 8.00 6.00 2.879 233 ctactatcatctctgcgcgatc 356 22 0 50.00 59.118 4.00 4.00 2.882 234 tgatcgatcgatgctagctagt 118 22 0 45.45 59.118 10.00 6.00 2.882 235 actgatcgatcgatgctagcta 116 22 0 45.45 59.118 10.00 6.00 2.882 236 tagctagctgatcgatcgatgt 71 22 0 45.45 59.118 10.00 6.00 2.882 237 tgatgctagtgatgcatgctag 20 22 0 45.45 59.115 8.00 8.00 2.885 238 ctgatgctagtgatgcatgcta 19 22 0 45.45 59.115 6.00 6.00 2.885 239 tagctgatgctagtgatgcatg 16 22 0 45.45 59.115 6.00 4.00 2.885 240 gctactatcatctctgcgcgat 355 22 0 50.00 60.887 4.00 4.00 2.887 241 tactatcatctctgcgcgatc 357 21 0 47.62 58.106 4.00 4.00 2.894 242 gactgatcgatcgatgctagct 115 22 0 50.00 60.895 10.00 6.00 2.895 243 gctgatgctagtgatgcatgc 18 21 0 52.38 61.911 6.00 6.00 2.911 244 gatgcatgcatgctactagc 449 20 0 50.00 57.086 10.00 7.00 2.914 245 gctgatcgatcgatgctagct 397 21 0 52.38 61.918 10.00 6.00 2.918 246 gctagctgatcgatcgatgct 393 21 0 52.38 61.918 10.00 8.00 2.918 247 agctagctgatcgatcgatgc 392 21 0 52.38 61.918 10.00 8.00 2.918 248 tgatgctagtgatgcatgcta 20 21 0 42.86 58.060 6.00 6.00 2.940 249 tatcatctctgcgcgatcga 360 20 0 50.00 62.940 6.00 4.00 2.940 250 tagctagctagctgatcgatcg 387 22 0 50.00 59.059 12.00 6.00 2.941 251 tagctagctagctgatcgatcg 67 22 0 50.00 59.059 12.00 6.00 2.941 252 cgatgtgcggctagctagct 87 20 0 60.00 62.979 10.00 8.00 2.979 253 ctgatacgcgatgctagctag 476 21 0 52.38 58.020 8.00 8.00 2.980 254 gcatgcatgctactagctagc 452 21 0 52.38 58.018 10.00 8.00 2.982 255 tcgtagcggcgatctagctag 575 21 0 57.14 61.990 8.00 8.00 2.990 256 gcatgcatgctactagctagct 452 22 0 50.00 58.997 10.00 8.00 3.003 257 atgctagctagctgatcgatcg 556 22 0 50.00 61.005 10.00 6.00 3.005 258 cgatgctagctagctgatcgat 554 22 0 50.00 61.005 10.00 8.00 3.005 259 cgatcatcgatgctagctagct 547 22 0 50.00 61.005 10.00 8.00 3.005 260 ctgatcgatcgatgctagctagt 117 23 0 47.83 60.041 10.00 6.00 3.041 261 actgatcgatcgatgctagctag 116 23 0 47.83 60.041 10.00 8.00 3.041 262 ctagctagctgatcgatcgatgt 70 23 0 47.83 60.041 10.00 6.00 3.041 263 gctgatcatcgatgctactagct 194 23 0 47.83 60.048 8.00 5.00 3.048 264 agctgatcatcgatgctactagc 193 23 0 47.83 60.048 8.00 8.00 3.048 265 gctagctgatcatcgatgctact 190 23 0 47.83 60.048 8.00 6.00 3.048 266 agctagctgatcatcgatgctac 189 23 0 47.83 60.048 8.00 6.00 3.048 267 ctagctagctagctgatcgatcg 386 23 0 52.17 59.952 12.00 6.00 3.048 268 ctagctagctagctgatcgatcg 66 23 0 52.17 59.952 12.00 6.00 3.048 269 tgatgctagtgatgcatgctagt 20 23 0 43.48 59.947 8.00 6.00 3.053 270 cgatcgatgctagtatgctagct 327 23 0 47.83 60.063 10.00 8.00 3.063 271 agctagctgatgctagtgatgc 13 22 0 50.00 58.936 8.00 2.00 3.064 272 gctagctagctgatcgatcgta 558 22 0 50.00 58.927 10.00 6.00 3.073 273 tcgatcgatgctagctagtagc 121 22 0 50.00 58.927 8.00 8.00 3.073 274 ctgatgctagtgatgcatgctag 19 23 0 47.83 60.074 8.00 8.00 3.074 275 ctagctgatgctagtgatgcatg 15 23 0 47.83 60.074 7.00 4.00 3.074 276 atctctgcgcgatcgatg 364 18 0 55.56 61.080 6.00 4.00 3.080 277 catctctgcgcgatcgat 363 18 0 55.56 61.080 6.00 6.00 3.080 278 atcatctctgcgcgatcg 361 18 0 55.56 61.080 6.00 6.00 3.080 279 gctagctgatgctagtgatgc 14 21 0 52.38 57.917 7.00 2.00 3.083 280 tagcggcgatctagctagctg 578 21 0 57.14 62.111 8.00 6.00 3.111 281 actgatacgcgatgctagcta 475 21 0 47.62 57.875 6.00 6.00 3.125 282 atgctagctaggcgatgct 409 19 0 52.63 57.861 8.00 3.00 3.139 283 actgatacgcgatgctagctag 475 22 0 50.00 58.860 8.00 8.00 3.140 284 catgcatgctactagctagctga 453 23 0 47.83 59.856 8.00 6.00 3.144 285 cggcgatctagctagctgacta 581 22 0 54.55 61.145 8.00 6.00 3.145 286 gatcgatgcatgcatgctacta 445 22 0 45.45 61.154 10.00 8.00 3.154 287 acatcgatcgatgctagtatgct 323 23 0 43.48 60.157 12.00 4.00 3.157 288 tcggattagctagctgatgctag 6 23 0 47.83 60.160 10.00 7.00 3.160 289 gctagctactatcatctctgcgc 351 23 0 52.17 59.838 6.00 4.00 3.162 290 gctagctagctactgatcgatgc 299 23 0 52.17 59.838 10.00 4.00 3.162 291 gctagctagctgatcgatcgtag 558 23 0 52.17 59.832 10.00 8.00 3.168 292 ctagctagctgatcatcgatgct 187 23 0 47.83 60.170 8.00 8.00 3.170 293 agctagctagctgatcatcgatg 185 23 0 47.83 60.170 12.00 8.00 3.170 294 gctagctgactagctgatcgatc 591 23 0 52.17 59.808 8.00 8.00 3.192 295 gctagctagctgactgatcgatc 104 23 0 52.17 59.808 10.00 8.00 3.192 296 cgcgatcgatgctagcta 371 18 0 55.56 58.807 6.00 6.00 3.193 297 gctatcgatcatcgatgctagc 542 22 0 50.00 61.206 11.00 8.00 3.206 298 gcatcggattagctagctgatg 3 22 0 50.00 61.220 10.00 8.00 3.220 299 tgactgatcgatcgatgct 114 19 0 47.37 57.768 10.00 6.00 3.232 300 ctgactgatcgatcgatgc 113 19 0 52.63 57.768 10.00 8.00 3.232 301 gctgactgatcgatcgatg 112 19 0 52.63 57.768 10.00 8.00 3.232 302 agctaggcgatgctagctagct 414 22 0 54.55 61.245 10.00 8.00 3.245 303 aggcgatgctagctagctact 418 21 0 52.38 57.752 10.00 6.00 3.248 304 tgactgatcgatcatcatgctag 266 23 0 43.48 60.250 12.00 5.00 3.250 305 ctgactgatcgatcatcatgcta 265 23 0 43.48 60.250 12.00 4.00 3.250 306 actagctagctgactgatacgcg 463 23 0 52.17 60.254 8.00 4.00 3.254 307 actgatcgatcatcatgctagct 268 23 0 43.48 60.271 10.00 5.00 3.271 308 ctagctgatcgatcgtagcgg 563 21 0 57.14 62.273 8.00 4.00 3.273 309 tagctgatcgatcgtagcg 564 19 0 52.63 57.716 8.00 4.00 3.284 310 gatcgatgtgcggctagctag 84 21 0 57.14 62.288 8.00 8.00 3.288 311 agctagctgactagctgatcga 590 22 0 50.00 58.678 9.00 4.00 3.322 312 agctagctagctgactgatcga 103 22 0 50.00 58.678 12.00 4.00 3.322 313 cgatcgtagcggcgatcta 572 19 0 57.89 62.347 8.00 4.00 3.347 314 catcggattagctagctgatg 4 21 0 47.62 57.647 10.00 8.00 3.353 315 gctagctgactagctgatcga 591 21 0 52.38 57.646 7.00 4.00 3.354 316 gctagctagctgactgatcga 104 21 0 52.38 57.646 10.00 4.00 3.354 317 atcgatcatcgatgctagctag 545 22 0 45.45 58.608 11.00 8.00 3.392 318 ctatcgatcatcgatgctagct 543 22 0 45.45 58.608 11.00 8.00 3.392 319 agctgactgatacgcgatgct 470 21 0 52.38 62.397 4.00 0.00 3.397 320 gctagctgatcgatcgtagcg 562 21 0 57.14 62.412 10.00 6.00 3.412 321 tcgatcgatgcatgcatg 443 18 0 50.00 61.413 10.00 8.00 3.413 322 catcgatcgatgcatgca 441 18 0 50.00 61.413 12.00 8.00 3.413 323 atcgatcatcgatgctagcta 545 21 0 42.86 57.587 11.00 6.00 3.413 324 tatcgatcatcgatgctagct 544 21 0 42.86 57.587 11.00 8.00 3.413 325 ctatcgatcatcgatgctagc 543 21 0 47.62 57.587 11.00 6.00 3.413 326 gctatcgatcatcgatgctag 542 21 0 47.62 57.587 11.00 8.00 3.413 327 tgctagctaggcgatgcta 410 19 0 52.63 57.552 8.00 5.00 3.448 328 ctgactgatacgcgatgct 472 19 0 52.63 57.520 4.00 0.00 3.480 329 agctgactgatacgcgatg 470 19 0 52.63 57.520 4.00 2.00 3.480 330 tgatcatcatcgatgctagcta 517 22 0 40.91 58.509 8.00 6.00 3.491 331 tagctgatcatcatcgatgcta 513 22 0 40.91 58.509 8.00 8.00 3.491 332 tgatcgatcatcatgctagcta 270 22 0 40.91 58.509 10.00 6.00 3.491 333 cggattagctagctgatgcta 7 21 0 47.62 57.498 10.00 6.00 3.502 334 tgatacgcgatgctagctagct 477 22 0 50.00 61.508 10.00 8.00 3.508 335 tgatcatcatcgatgctagctag 517 23 0 43.48 59.492 8.00 8.00 3.508 336 ctgatcatcatcgatgctagcta 516 23 0 43.48 59.492 8.00 6.00 3.508 337 tagctgatcatcatcgatgctag 513 23 0 43.48 59.492 8.00 6.00 3.508 338 ctagctgatcatcatcgatgcta 512 23 0 43.48 59.492 8.00 8.00 3.508 339 tagctagctgatcatcatcgatg 509 23 0 43.48 59.492 8.00 8.00 3.508 340 ctgatcgatcatcatgctagcta 269 23 0 43.48 59.492 10.00 6.00 3.508 341 cggattagctagctgatgctag 7 22 0 50.00 58.481 10.00 7.00 3.519 342 gcggcgatctagctagct 580 18 0 61.11 58.478 8.00 8.00 3.522 343 agcggcgatctagctagc 579 18 0 61.11 58.478 8.00 6.00 3.522 344 cgatcgatgtgcggctag 83 18 0 61.11 61.523 6.00 4.00 3.523 345 aaagcatcggattagctagct 0 21 0 42.86 57.475 8.00 8.00 3.525 346 tgctagctagctgatcgatcgt 557 22 0 50.00 61.537 10.00 4.00 3.537 347 agctactatcatctctgcgcga 354 22 0 50.00 61.537 4.00 4.00 3.537 348 gctgactagctgatcgatcgtag 595 23 0 52.17 60.556 8.00 8.00 3.556 349 agctgactagctgatcgatcgta 594 23 0 47.83 60.556 8.00 6.00 3.556 350 tagctgactagctgatcgatcgt 593 23 0 47.83 60.556 8.00 4.00 3.556 351 gactgatacgcgatgctagctag 474 23 0 52.17 60.560 8.00 8.00 3.560 352 tagctagctgactgatacgcgat 465 23 0 47.83 60.560 8.00 4.00 3.560 353 agctagctgactgatcgatcga 107 22 0 50.00 61.569 8.00 8.00 3.569 354 atcatcgatgctagctagctg 549 21 0 47.62 57.405 10.00 6.00 3.595 355 catcgatcgatgctagtatgcta 324 23 0 43.48 59.402 12.00 6.00 3.598 356 gcgcgatcgatgctagcta 370 19 0 57.89 62.627 6.00 6.00 3.627 357 tcgatgctagctaggcga 406 18 0 55.56 58.362 8.00 4.00 3.638 358 tgatcgatcatcatgctagctac 270 23 0 43.48 59.360 10.00 4.00 3.640 359 gctagctgactgatacgcg 467 19 0 57.89 57.357 6.00 4.00 3.643 360 gatgctagctagctgatcgatc 555 22 0 50.00 58.355 10.00 8.00 3.645 361 ctagctactatcatctctgcgcg 352 23 0 52.17 60.675 6.00 4.00 3.675 362 ctagctgactagctgatcgatcg 592 23 0 52.17 60.675 8.00 6.00 3.675 363 ctagctagctgactgatcgatcg 105 23 0 52.17 60.675 8.00 6.00 3.675 364 atcgatcgatgtgcggcta 81 19 0 52.63 62.688 10.00 2.00 3.688 365 atcgatcgtagcggcgat 570 18 0 55.56 61.698 6.00 6.00 3.698 366 cgatgtgcggctagctag 87 18 0 61.11 58.288 8.00 8.00 3.712 367 actatcatctctgcgcgatcg 358 21 0 52.38 62.718 6.00 6.00 3.718 368 tgatcgatcgatgctagctagg 399 22 0 50.00 61.720 10.00 6.00 3.720 369 tacatcgatcgatgctagtatgc 322 23 0 43.48 59.274 12.00 4.00 3.726 370 gatgcatgcatgctactagcta 449 22 0 45.45 58.265 10.00 6.00 3.735 371 cggattagctagctgatgctagt 7 23 0 47.83 59.261 10.00 6.00 3.739 372 ctactatcatctctgcgcgat 356 21 0 47.62 57.255 4.00 4.00 3.745 373 tagctagctagctgatcgatcga 387 23 0 47.83 60.752 12.00 8.00 3.752 374 tagctagctagctgatcgatcga 67 23 0 47.83 60.752 12.00 8.00 3.752 375 gatgcatgcatgctactagctag 449 23 0 47.83 59.227 10.00 8.00 3.773 376 atcgatcgatgctagctagtagc 120 23 0 47.83 59.226 10.00 8.00 3.774 377 agctactgatcgatgctacatcg 306 23 0 47.83 60.793 8.00 8.00 3.793 378 agctagctgactgatcgatcat 259 22 0 45.45 58.201 10.00 8.00 3.799 379 agctagctgatcgatcgatgtg 72 22 0 50.00 61.811 10.00 8.00 3.811 380 ctgcgcgatcgatgctag 368 18 0 61.11 61.813 6.00 4.00 3.813 381 gctgactgatcgatcgatgct 112 21 0 52.38 62.815 10.00 6.00 3.815 382 agctgactgatcgatcgatgc 111 21 0 52.38 62.815 10.00 8.00 3.815 383 gctgatcatcatcgatgct 515 19 0 47.37 57.176 8.00 8.00 3.824 384 agctgatcatcatcgatgc 514 19 0 47.37 57.176 8.00 6.00 3.824 385 atcgatgctacatcgatcg 314 19 0 47.37 57.160 12.00 6.00 3.840 386 tgatcgatcgatgctagctagta 118 23 0 43.48 59.159 10.00 8.00 3.841 387 gctgatcgatcgatgctagcta 397 22 0 50.00 61.859 10.00 6.00 3.859 388 tagctagctgatcgatcgatgc 391 22 0 50.00 61.859 10.00 8.00 3.859 389 agctagctagctgatcatcga 185 21 0 47.62 57.134 12.00 6.00 3.866 390 gactgatcgatcgatgctagcta 115 23 0 47.83 60.873 10.00 6.00 3.873 391 gctagctgactgatcgatcat 260 21 0 47.62 57.117 10.00 8.00 3.883 392 cgatcgatgctagctagtagc 122 21 0 52.38 57.116 8.00 8.00 3.884 393 catgcatgctactagctagctg 453 22 0 50.00 58.085 8.00 8.00 3.915 394 atgcatgcatgctactagctagc 450 23 0 47.83 60.921 10.00 8.00 3.921 395 gctagctgatgctagtgatgcat 14 23 0 47.83 60.929 7.00 6.00 3.929 396 gctagctgactagctgatcgat 591 22 0 50.00 58.034 7.00 6.00 3.966 397 gctagctagctgactgatcgat 104 22 0 50.00 58.034 10.00 6.00 3.966 398 tgcatgcatgctactagctag 451 21 0 47.62 57.032 10.00 8.00 3.968 399 tcgatcatcgatgctagct 546 19 0 47.37 57.026 9.00 4.00 3.974 400 tgatcgatcgatgctagct 399 19 0 47.37 57.026 10.00 4.00 3.974 401 ctgatcgatcgatgctagc 398 19 0 52.63 57.026 10.00 6.00 3.974 402 gctgatcgatcgatgctag 397 19 0 52.63 57.026 10.00 8.00 3.974 403 agctgatcgatcgatgcta 396 19 0 47.37 57.026 10.00 6.00 3.974 404 tagctgatcgatcgatgct 395 19 0 47.37 57.026 10.00 8.00 3.974 405 ctagctgatcgatcgatgc 394 19 0 52.63 57.026 10.00 8.00 3.974 406 gctagctgatcgatcgatg 393 19 0 52.63 57.026 10.00 8.00 3.974 407 ctatcatctctgcgcgatc 359 19 0 52.63 57.026 4.00 4.00 3.974 408 tgatcgatcgatgctagct 118 19 0 47.37 57.026 10.00 4.00 3.974 409 ctgatcgatcgatgctagc 117 19 0 52.63 57.026 10.00 6.00 3.974 410 gctagctgatcgatcgatg 73 19 0 52.63 57.026 10.00 8.00 3.974 411 gctagctgatcatcgatgc 190 19 0 52.63 57.021 8.00 6.00 3.979 412 tcatcgatgctagctagctgatc 550 23 0 47.83 61.000 10.00 8.00 4.000 413 gatcatcgatgctagctagctga 548 23 0 47.83 61.000 10.00 8.00 4.000 414 agctagctgactagctgatcgat 590 23 0 47.83 58.998 9.00 6.00 4.002 415 agctagctagctgactgatcgat 103 23 0 47.83 58.998 12.00 6.00 4.002 416 tagctagctgatgctagtgatgc 12 23 0 47.83 58.984 8.00 2.00 4.016 417 cgatgctactagctagctgatca 203 23 0 47.83 58.981 8.00 6.00 4.019 418 catcgatgctactagctagctga 200 23 0 47.83 58.981 8.00 6.00 4.019 419 tcatcgatgctactagctagctg 199 23 0 47.83 58.981 8.00 7.00 4.019 420 tgatgctagtgatgcatgctagta 20 24 0 41.67 59.959 8.00 8.00 4.041 421 tgatcgatcgatgctagctagtag 118 24 0 45.83 60.049 10.00 6.00 4.049 422 ctgatcgatcgatgctagctagta 117 24 0 45.83 60.049 10.00 8.00 4.049 423 tcatcatcgatgctagctagtagc 520 24 0 45.83 60.056 8.00 8.00 4.056 424 tcgatcatcatgctagctactagc 273 24 0 45.83 60.056 8.00 8.00 4.056 425 tgatcatcgatgctactagctagc 196 24 0 45.83 60.056 8.00 8.00 4.056 426 gctgatcatcgatgctactagcta 194 24 0 45.83 60.056 8.00 6.00 4.056 427 tagctgatcatcgatgctactagc 192 24 0 45.83 60.056 8.00 8.00 4.056 428 gctagctgatcatcgatgctacta 190 24 0 45.83 60.056 8.00 8.00 4.056 429 tagctagctgatcatcgatgctac 188 24 0 45.83 60.056 10.00 6.00 4.056 430 cgatcgatgctagtatgctagcta 327 24 0 45.83 60.070 10.00 8.00 4.070 431 gctaggcgatgctagctagctac 415 23 0 56.52 61.109 10.00 8.00 4.109 432 ggcgatctagctagctgactagc 582 23 0 56.52 61.123 8.00 8.00 4.123 433 gcgatctagctagctgactagc 583 22 0 54.55 57.876 8.00 8.00 4.124 434 gactgatcgatcatcatgctagc 267 23 0 47.83 61.135 10.00 6.00 4.135 435 atcgtagcggcgatctagctag 574 22 0 54.55 62.138 8.00 8.00 4.138 436 gctagctactgatcgatgctaca 303 23 0 47.83 58.849 6.00 3.00 4.151 437 gctgatcatcatcgatgctagc 515 22 0 50.00 62.158 8.00 8.00 4.158 438 gctagctgatcatcatcgatgc 511 22 0 50.00 62.158 8.00 7.00 4.158 439 acatcgatcgatgctagtatgcta 323 24 0 41.67 60.161 12.00 6.00 4.161 440 tacatcgatcgatgctagtatgct 322 24 0 41.67 60.161 12.00 4.00 4.161 441 aggcgatgctagctagctacta 418 22 0 50.00 57.839 10.00 8.00 4.161 442 taggcgatgctagctagctact 417 22 0 50.00 57.839 10.00 6.00 4.161 443 ctaggcgatgctagctagctac 416 22 0 54.55 57.839 10.00 8.00 4.161 444 tagctagctagctgatcatcgatg 184 24 0 45.83 60.174 12.00 8.00 4.174 445 ctagctagctgactgatacgcga 464 23 0 52.17 61.184 8.00 3.00 4.184 446 ttagctagctgatgctagtgatgc 11 24 0 45.83 60.212 8.00 2.00 4.212 447 tagctaggcgatgctagctagct 413 23 0 52.17 61.214 10.00 8.00 4.214 448 agctagctactgatcgatgctaca 302 24 0 45.83 59.754 8.00 2.00 4.246 449 ctaggcgatgctagctagctact 416 23 0 52.17 58.754 10.00 6.00 4.246 450 tactagctagctgactgatacgcg 462 24 0 50.00 60.255 8.00 4.00 4.255 451 tcgatgcatgcatgctac 447 18 0 50.00 57.745 10.00 8.00 4.255 452 ggcgatctagctagctgactag 582 22 0 54.55 57.739 8.00 8.00 4.261 453 tagctagctgactagctgatcga 589 23 0 47.83 58.736 9.00 4.00 4.264 454 tagctagctagctgactgatcga 102 23 0 47.83 58.736 12.00 4.00 4.264 455 actatcatcgatcgatgcatgc 436 22 0 45.45 62.270 12.00 8.00 4.270 456 tgatcatcatcgatgctagctagt 517 24 0 41.67 60.271 8.00 6.00 4.271 457 tgatcgatcatcatgctagctact 270 24 0 41.67 60.271 10.00 2.00 4.271 458 ctgatcgatcatcatgctagctac 269 24 0 45.83 60.271 10.00 4.00 4.271 459 actgatcgatcatcatgctagcta 268 24 0 41.67 60.271 10.00 6.00 4.271 460 catcgatcgatgctagtatgctag 324 24 0 45.83 60.278 12.00 8.00 4.278 461 tgcatgctactagctagctgac 455 22 0 50.00 57.681 8.00 6.00 4.319 462 tgactgatacgcgatgctagct 473 22 0 50.00 62.319 6.00 4.00 4.319 463 ctgactgatacgcgatgctagc 472 22 0 54.55 62.319 6.00 6.00 4.319 464 gctgactgatacgcgatgctag 471 22 0 54.55 62.319 4.00 4.00 4.319 465 agctgactgatacgcgatgcta 470 22 0 50.00 62.319 4.00 2.00 4.319 466 tagctgactgatacgcgatgct 469 22 0 50.00 62.319 4.00 0.00 4.319 467 ctagctgactgatacgcgatgc 468 22 0 54.55 62.319 4.00 2.00 4.319 468 gctagctgactgatacgcgatg 467 22 0 54.55 62.319 6.00 2.00 4.319 469 gcatgctactagctagctgactg 456 23 0 52.17 58.672 8.00 6.00 4.328 470 tgcatgctactagctagctgact 455 23 0 47.83 58.672 8.00 6.00 4.328 471 tatcgatcatcgatgctagctag 544 23 0 43.48 58.667 11.00 8.00 4.333 472 tgctagtgatgcatgctagtagtg 23 24 0 45.83 59.645 8.00 5.00 4.355 473 ctagctagctgactagctgatcga 588 24 0 50.00 59.644 9.00 4.00 4.356 474 tctagctagctgactagctgatcg 587 24 0 50.00 59.644 9.00 4.00 4.356 475 ctagctagctagctgactgatcga 101 24 0 50.00 59.644 12.00 4.00 4.356 476 gcatgctactagctagctgactga 456 24 0 50.00 60.373 8.00 4.00 4.373 477 atcggattagctagctgatgctag 5 24 0 45.83 60.387 10.00 8.00 4.387 478 ctgatcatcatcgatgctagctag 516 24 0 45.83 60.391 8.00 8.00 4.391 479 ctagctagctgatcatcatcgatg 508 24 0 45.83 60.391 8.00 8.00 4.391 480 agctgatcgatcgtagcg 565 18 0 55.56 57.606 8.00 4.00 4.394 481 ttagctagctgactgatcgatcat 257 24 0 41.67 59.570 10.00 8.00 4.430 482 cgatcgtagcggcgatct 572 18 0 61.11 62.431 8.00 6.00 4.431 483 agctagctagctgatcatcgat 185 22 0 45.45 57.542 12.00 6.00 4.458 484 tgctagctagctgatcgatcgta 557 23 0 47.83 61.493 10.00 6.00 4.493 485 tagctactatcatctctgcgcga 353 23 0 47.83 61.493 6.00 4.00 4.493 486 gatcgatcgatgctagctaggc 400 22 0 54.55 62.516 10.00 6.00 4.516 487 tagctagctgactgatcgatcga 106 23 0 47.83 61.523 8.00 8.00 4.523 488 gatcgtagcggcgatcta 573 18 0 55.56 57.475 8.00 2.00 4.525 489 tcgatcgatgtgcggcta 82 18 0 55.56 62.543 8.00 2.00 4.543 490 gcatgcatgctactagctagctg 452 23 0 52.17 61.544 10.00 8.00 4.544 491 tgcatgcatgctactagctagct 451 23 0 47.83 61.544 10.00 8.00 4.544 492 tagctgactagctgatcgatcgta 593 24 0 45.83 60.546 8.00 6.00 4.546 493 atgcatgcatgctactagctag 450 22 0 45.45 57.449 10.00 8.00 4.551 494 cggcgatctagctagctg 581 18 0 61.11 57.442 8.00 6.00 4.558 495 tgctagctaggcgatgct 410 18 0 55.56 57.439 8.00 3.00 4.561 496 agctagctgatgctagtgatgca 13 23 0 47.83 61.574 8.00 4.00 4.574 497 catgcatgctactagctagctgac 453 24 0 50.00 60.603 8.00 6.00 4.603 498 ctagctagctgatcatcatcga 508 22 0 45.45 57.392 8.00 7.00 4.608 499 gactgatcgatcatcatgctag 267 22 0 45.45 57.387 10.00 5.00 4.613 500 tactatcatctctgcgcgatcg 357 22 0 50.00 62.629 6.00 6.00 4.629 501 tcgatcgatgctagtatgctag 326 22 0 45.45 57.367 8.00 8.00 4.633 502 aaagcatcggattagctagctga 0 23 0 43.48 61.634 8.00 8.00 4.634 503 catcatcgatgctagctagtagc 521 23 0 47.83 58.360 8.00 8.00 4.640 504 cgatcatcatgctagctactagc 274 23 0 47.83 58.360 8.00 8.00 4.640 505 tcgatgctactagctagctgatca 202 24 0 45.83 60.670 8.00 6.00 4.670 506 ctagctgatgctagtgatgcat 15 22 0 45.45 57.329 7.00 6.00 4.671 507 agctagctgactagctgatcgatc 590 24 0 50.00 60.672 9.00 8.00 4.672 508 agctagctagctgactgatcgatc 103 24 0 50.00 60.672 12.00 8.00 4.672 509 agctagctactatcatctctgcgc 350 24 0 50.00 60.672 8.00 4.00 4.672 510 gctagctagctactgatcgatgct 299 24 0 50.00 60.672 10.00 4.00 4.672 511 agctagctagctactgatcgatgc 298 24 0 50.00 60.672 12.00 4.00 4.672 512 cgatgcatgcatgctactagct 448 22 0 50.00 62.673 10.00 5.00 4.673 513 atgctagctagctgatcgatcgt 556 23 0 47.83 61.711 10.00 4.00 4.711 514 agctactatcatctctgcgcgat 354 23 0 47.83 61.711 4.00 4.00 4.711 515 tgactgatcgatcgatgctagc 114 22 0 50.00 62.719 10.00 6.00 4.719 516 gctgactgatcgatcgatgcta 112 22 0 50.00 62.719 10.00 6.00 4.719 517 tagctgactgatcgatcgatgc 110 22 0 50.00 62.719 10.00 8.00 4.719 518 gcgcgatcgatgctagct 370 18 0 61.11 62.722 6.00 4.00 4.722 519 actagctagctgatcatcatcga 507 23 0 43.48 58.277 8.00 7.00 4.723 520 tagctagctgactgatcgatcat 258 23 0 43.48 58.277 10.00 8.00 4.723 521 cgatgctactagctagctgatcat 203 24 0 45.83 59.274 8.00 8.00 4.726 522 catcgatgctactagctagctgat 200 24 0 45.83 59.274 8.00 8.00 4.726 523 atcatcgatgctactagctagctg 198 24 0 45.83 59.274 8.00 7.00 4.726 524 agctagctgatcgatcgatgct 392 22 0 50.00 62.732 10.00 8.00 4.732 525 ggattagctagctgatgctagtga 8 24 0 45.83 59.252 10.00 5.00 4.748 526 tagctagctagctgatcatcga 184 22 0 45.45 57.250 12.00 6.00 4.750 527 ctagctagctagctgatcatcga 183 23 0 47.83 58.249 12.00 6.00 4.751 528 tagctagctgatcgatcgatgtg 71 23 0 47.83 61.756 10.00 8.00 4.756 529 gctgatgctagtgatgcatgct 18 22 0 50.00 62.764 6.00 4.00 4.764 530 agctgatgctagtgatgcatgc 17 22 0 50.00 62.764 6.00 6.00 4.764 531 gtagcggcgatctagctagctg 577 22 0 59.09 62.774 8.00 6.00 4.774 532 tagctactgatcgatgctacatcg 305 24 0 45.83 60.775 8.00 8.00 4.775 533 actgatcgatcgatgctagctagt 116 24 0 45.83 60.775 10.00 6.00 4.775 534 atcgatcgatgtgcggct 81 18 0 55.56 62.790 10.00 1.00 4.790 535 catcgatgctactagctagctg 200 22 0 50.00 57.193 8.00 7.00 4.807 536 atgcatgctactagctagctga 454 22 0 45.45 57.189 8.00 6.00 4.811 537 gatgctagtgatgcatgctagt 21 22 0 45.45 57.176 8.00 6.00 4.824 538 tctgcgcgatcgatgcta 367 18 0 55.56 62.829 6.00 2.00 4.829 539 ctgatgctagtgatgcatgctagt 19 24 0 45.83 60.835 8.00 6.00 4.835 540 gatcgatcgatgctagctagtagc 119 24 0 50.00 60.846 10.00 8.00 4.846 541 gctagctactgatcgatgctacat 303 24 0 45.83 59.150 6.00 4.00 4.850 542 atcgatgcatgcatgcta 446 18 0 44.44 57.148 10.00 8.00 4.852 543 tcggattagctagctgatgctagt 6 24 0 45.83 60.863 10.00 6.00 4.863 544 tcgatgctactagctagctgatc 202 23 0 47.83 58.113 8.00 8.00 4.887 545 tgctagctaggcgatgctagct 410 22 0 54.55 62.888 9.00 8.00 4.888 546 agctagctgatcatcgatgctact 189 24 0 45.83 60.897 8.00 7.00 4.897 547 tcgatgctagtatgctagctagct 330 24 0 45.83 59.095 10.00 8.00 4.905 548 tgatcgatcgatgtgcgg 79 18 0 55.56 62.906 10.00 1.00 4.906 549 tcatctctgcgcgatcga 362 18 0 55.56 62.924 6.00 4.00 4.924 550 cggcgatctagctagctgactag 581 23 0 56.52 61.940 8.00 8.00 4.940 551 atgcatgctactagctagctgac 454 23 0 47.83 58.052 8.00 6.00 4.948 552 tagctagctgactagctgatcgat 589 24 0 45.83 59.042 9.00 6.00 4.958 553 tagctagctagctgactgatcgat 102 24 0 45.83 59.042 12.00 6.00 4.958 554 gatcgtagcggcgatctagcta 573 22 0 54.55 62.965 8.00 6.00 4.965 555 gatcgatgcatgcatgctactag 445 23 0 47.83 62.001 10.00 6.00 5.001 556 agctagctactgatcgatgctacat 302 25 0 44.00 60.001 8.00 4.00 5.001 557 gctatcgatcatcgatgctagct 542 23 0 47.83 62.017 11.00 8.00 5.017 558 atgcatgctactagctagctgact 454 24 0 45.83 58.980 8.00 6.00 5.020 559 catcggattagctagctgatgct 4 23 0 47.83 62.021 10.00 8.00 5.021 560 agcatcggattagctagctgatg 2 23 0 47.83 62.021 10.00 8.00 5.021 561 tttagctagctgactgatcgatc 256 23 0 43.48 57.951 8.00 8.00 5.049 562 gctatttagctagctgactgatcg 252 24 0 45.83 58.950 8.00 3.00 5.050 563 ctagctagctgactagctgatcg 588 23 0 52.17 57.942 9.00 3.00 5.058 564 cgatctagctagctgactagctg 584 23 0 52.17 57.942 9.00 7.00 5.058 565 ctagctagctagctgactgatcg 101 23 0 52.17 57.942 12.00 2.00 5.058 566 taggcgatgctagctagctacta 417 23 0 47.83 57.921 10.00 8.00 5.079 567 gctagctagtagctatcgatcatcg 531 25 0 48.00 60.083 11.00 7.00 5.083 568 gctagctagctactatcatcgatcg 425 25 0 48.00 60.083 10.00 6.00 5.083 569 atgctagtgatgcatgctagtagtg 22 25 0 44.00 59.907 8.00 4.00 5.093 570 ctagctagctgactagctgatcgat 588 25 0 48.00 59.895 9.00 6.00 5.105 571 atctagctagctgactagctgatcg 586 25 0 48.00 59.895 11.00 6.00 5.105 572 ctagctagctagctgactgatcgat 101 25 0 48.00 59.895 12.00 6.00 5.105 573 ctgactgatcgatcatcatgctag 265 24 0 45.83 61.155 12.00 6.00 5.155 574 tacatcgatcgatgctagtatgcta 322 25 0 40.00 60.165 12.00 6.00 5.165 575 ctaggcgatgctagctagctacta 416 24 0 50.00 58.803 10.00 8.00 5.197 576 tactatcatcgatcgatgcatgc 435 23 0 43.48 62.198 12.00 8.00 5.198 577 atcatcgatgctagctagctgatc 549 24 0 45.83 61.198 10.00 8.00 5.198 578 ctagctagctgatcatcatcgat 508 23 0 43.48 57.785 8.00 6.00 5.215 579 agctatttagctagctgactgatcg 251 25 0 44.00 59.785 8.00 5.00 5.215 580 tgcatgctactagctagctgactg 455 24 0 50.00 61.222 8.00 6.00 5.222 581 gctagtgatgcatgctagtagtga 24 24 0 45.83 58.772 8.00 2.00 5.228 582 tagctagctactgatcgatgctaca 301 25 0 44.00 59.772 10.00 2.00 5.228 583 tgactgatacgcgatgctagcta 473 23 0 47.83 62.247 6.00 6.00 5.247 584 tagctgactgatacgcgatgcta 469 23 0 47.83 62.247 4.00 2.00 5.247 585 atcgatcgatgctagtatgctag 325 23 0 43.48 57.749 10.00 8.00 5.251 586 gctactagctagctgactgatacgc 460 25 0 52.00 60.268 8.00 4.00 5.268 587 gcgatctagctagctgactagctg 583 24 0 54.17 61.268 9.00 7.00 5.268 588 tgatcatcatcgatgctagctagta 517 25 0 40.00 60.272 8.00 8.00 5.272 589 tgatcgatcatcatgctagctacta 270 25 0 40.00 60.272 10.00 4.00 5.272 590 ctgatacgcgatgctagctagct 476 23 0 52.17 62.283 10.00 8.00 5.283 591 atcatcatcgatgctagctagtagc 519 25 0 44.00 60.289 8.00 8.00 5.289 592 atcgatcatcatgctagctactagc 272 25 0 44.00 60.289 8.00 8.00 5.289 593 gatcatcatcgatgctagctagt 518 23 0 43.48 57.643 8.00 6.00 5.357 594 gatcgatcatcatgctagctact 271 23 0 43.48 57.643 8.00 2.00 5.357 595 tagctagctagctgatcatcgat 184 23 0 43.48 57.639 12.00 6.00 5.361 596 ctagctagctgactgatacgcgat 464 24 0 50.00 61.368 8.00 4.00 5.368 597 actagctagctgatcatcatcgat 507 24 0 41.67 58.612 8.00 6.00 5.388 598 agctgactagctgatcgatcgtag 594 24 0 50.00 61.392 8.00 8.00 5.392 599 ctagctgactagctgatcgatcgt 592 24 0 50.00 61.392 8.00 4.00 5.392 600 ctagctagctagctgatcatcgat 183 24 0 45.83 58.574 12.00 6.00 5.426 601 attagctagctgatgctagtgatgc 10 25 0 44.00 60.436 10.00 2.00 5.436 602 ggattagctagctgatgctagtg 8 23 0 47.83 57.556 10.00 4.00 5.444 603 ggattagctagctgatgctagtgat 8 25 0 44.00 59.520 10.00 7.00 5.480 604 agctagctactatcatcgatcga 428 23 0 43.48 57.502 8.00 8.00 5.498 605 gatcgatcgatgctagctagtag 119 23 0 47.83 57.502 10.00 6.00 5.498 606 gatcatcgatgctactagctagc 197 23 0 47.83 57.499 8.00 8.00 5.501 607 ctgatcgatcgatgctagctagg 398 23 0 52.17 62.515 10.00 6.00 5.515 608 tgctactagctagctgactgatacg 459 25 0 48.00 59.481 8.00 2.00 5.519 609 gctatttagctagctgactgatcga 252 25 0 44.00 60.527 8.00 4.00 5.527 610 tagctagctgatgctagtgatgca 12 24 0 45.83 61.529 8.00 4.00 5.529 611 gctactatcatctctgcgcgatc 355 23 0 52.17 62.545 4.00 4.00 5.545 612 ctagctagctagctgatcgatcga 386 24 0 50.00 61.552 12.00 8.00 5.552 613 ctagctagctagctgatcgatcga 66 24 0 50.00 61.552 12.00 8.00 5.552 614 atcgatgctactagctagctgatc 201 24 0 45.83 58.447 8.00 8.00 5.553 615 gatcatcgatgctactagctagct 197 24 0 45.83 58.447 8.00 8.00 5.553 616 cgatgctagtatgctagctagct 331 23 0 47.83 57.446 10.00 8.00 5.554 617 cgatgcatgcatgctactagcta 448 23 0 47.83 62.589 10.00 6.00 5.589 618 gcatgctactagctagctgactgat 456 25 0 48.00 60.594 8.00 4.00 5.594 619 atcgatgctagtatgctagctagct 329 25 0 44.00 59.362 10.00 8.00 5.638 620 gatgctagctagctgatcgatcg 555 23 0 52.17 62.647 10.00 6.00 5.647 621 tcgatcatcgatgctagctagct 546 23 0 47.83 62.647 10.00 8.00 5.647 622 gctgatcgatcgatgctagctag 397 23 0 52.17 62.647 10.00 8.00 5.647 623 agctgatcgatcgatgctagcta 396 23 0 47.83 62.647 12.00 8.00 5.647 624 tagctagctgatcgatcgatgct 391 23 0 47.83 62.647 10.00 8.00 5.647 625 ctagctagctgatcgatcgatgc 390 23 0 52.17 62.647 10.00 8.00 5.647 626 gctagctagctgatcgatcgatg 389 23 0 52.17 62.647 10.00 8.00 5.647 627 gctagctagctgatcgatcgatg 69 23 0 52.17 62.647 10.00 8.00 5.647 628 tactagctagctgatcatcatcga 506 24 0 41.67 58.348 8.00 7.00 5.652 629 tagctagctgactagctgatcgatc 589 25 0 48.00 60.659 9.00 8.00 5.659 630 tagctagctagctgactgatcgatc 102 25 0 48.00 60.659 12.00 8.00 5.659 631 tagctagctactatcatctctgcgc 349 25 0 48.00 60.659 10.00 4.00 5.659 632 gctagctagctactgatcgatgcta 299 25 0 48.00 60.659 10.00 6.00 5.659 633 gctagctagctgatcatcgatgc 186 23 0 52.17 62.663 10.00 6.00 5.663 634 atgctagctagctgatcgatcgta 556 24 0 45.83 61.663 10.00 6.00 5.663 635 tagctactatcatctctgcgcgat 353 24 0 45.83 61.663 6.00 4.00 5.663 636 tcgatcgatgctagtatgctagct 326 24 0 45.83 61.663 10.00 8.00 5.663 637 agctagctactatcatctctgcg 350 23 0 47.83 57.335 8.00 2.00 5.665 638 ctagctagctactgatcgatgct 300 23 0 47.83 57.335 10.00 4.00 5.665 639 agctagctagctactgatcgatg 298 23 0 47.83 57.335 12.00 4.00 5.665 640 cggattagctagctgatgctagtg 7 24 0 50.00 61.671 10.00 4.00 5.671 641 gctgatgctagtgatgcatgcta 18 23 0 47.83 62.674 6.00 6.00 5.674 642 tagctgatgctagtgatgcatgc 16 23 0 47.83 62.674 6.00 6.00 5.674 643 gactgatcgatcgatgctagctag 115 24 0 50.00 61.697 10.00 8.00 5.697 644 atttagctagctgactgatcgatc 255 24 0 41.67 58.295 8.00 8.00 5.705 645 atgcatgcatgctactagctagct 450 24 0 45.83 61.710 10.00 8.00 5.710 646 gatgctagtgatgcatgctagta 21 23 0 43.48 57.290 8.00 8.00 5.710 647 gatgctagtgatgcatgctagtag 21 24 0 45.83 58.286 8.00 6.00 5.714 648 tgctactagctagctgatcatcatc 206 25 0 44.00 59.265 8.00 7.00 5.735 649 gatgctactagctagctgatcatca 204 25 0 44.00 59.265 10.00 8.00 5.735 650 ctactagctagctgatcatcatcga 505 25 0 44.00 59.261 8.00 7.00 5.739 651 tttagctagctgactgatcgatcat 256 25 0 40.00 60.741 10.00 8.00 5.741 652 agctagctgatgctagtgatgcat 13 24 0 45.83 61.745 8.00 6.00 5.745 653 actgatcgatcgatgctagctagta 116 25 0 44.00 60.759 10.00 8.00 5.759 654 gctagctactgatcgatgctacatc 303 25 0 48.00 60.768 6.00 6.00 5.768 655 agctagctactgatcgatgctac 302 23 0 47.83 57.193 8.00 4.00 5.807 656 tgatgctagtgatgcatgctagtag 20 25 0 44.00 60.816 8.00 6.00 5.816 657 ctgatgctagtgatgcatgctagta 19 25 0 44.00 60.816 8.00 8.00 5.816 658 tgctactagctagctgatcatca 206 23 0 43.48 57.177 8.00 7.00 5.823 659 gctaggcgatgctagctagctact 415 24 0 54.17 61.851 10.00 6.00 5.851 660 agctaggcgatgctagctagctac 414 24 0 54.17 61.851 10.00 8.00 5.851 661 cgatcgatgctagtatgctagctag 327 25 0 48.00 60.861 10.00 8.00 5.861 662 ctgatcgatcgatgctagctagtag 117 25 0 48.00 60.867 10.00 6.00 5.867 663 actagctagctgactgatacgcga 463 24 0 50.00 61.869 8.00 3.00 5.869 664 gctactagctagctgatcatcatcg 504 25 0 48.00 60.876 8.00 5.00 5.876 665 tcgatgctactagctagctgatcat 202 25 0 44.00 60.876 8.00 8.00 5.876 666 atcgatgctactagctagctgatca 201 25 0 44.00 60.876 8.00 6.00 5.876 667 catcgatgctactagctagctgatc 200 25 0 48.00 60.876 8.00 8.00 5.876 668 tcatcgatgctactagctagctgat 199 25 0 44.00 60.876 9.00 8.00 5.876 669 gatcatcgatgctactagctagctg 197 25 0 48.00 60.876 8.00 7.00 5.876 670 tgatcatcgatgctactagctagct 196 25 0 44.00 60.876 8.00 8.00 5.876 671 ctgatcatcgatgctactagctagc 195 25 0 48.00 60.876 8.00 8.00 5.876 672 gctgatcatcgatgctactagctag 194 25 0 48.00 60.876 8.00 8.00 5.876 673 agctgatcatcgatgctactagcta 193 25 0 44.00 60.876 10.00 6.00 5.876 674 agctagctgatcatcgatgctacta 189 25 0 44.00 60.876 8.00 8.00 5.876 675 tagctagctgatcatcgatgctact 188 25 0 44.00 60.876 10.00 7.00 5.876 676 ctagctagctgatcatcgatgctac 187 25 0 48.00 60.876 10.00 8.00 5.876 677 agtgatgcatgctagtagtgatg 27 23 0 43.48 57.104 6.00 5.00 5.896 678 gctagtgatgcatgctagtagtgat 24 25 0 44.00 59.076 8.00 4.00 5.924 679 gatgctagtgatgcatgctagtagt 21 25 0 44.00 59.076 8.00 6.00 5.924 680 ctagctaggcgatgctagctagct 412 24 0 54.17 61.947 12.00 8.00 5.947 681 gctgatcatcatcgatgctagct 515 23 0 47.83 62.960 8.00 6.00 5.960 682 agctgatcatcatcgatgctagc 514 23 0 47.83 62.960 8.00 8.00 5.960 683 gctagctgatcatcatcgatgct 511 23 0 47.83 62.960 8.00 8.00 5.960 684 agctagctgatcatcatcgatgc 510 23 0 47.83 62.960 8.00 7.00 5.960 685 gactgatcgatcatcatgctagct 267 24 0 45.83 61.975 10.00 5.00 5.975 686 acatcgatcgatgctagtatgctag 323 25 0 44.00 60.976 12.00 8.00 5.976 687 ctagctagctagctgatcatcgatg 183 25 0 48.00 60.984 12.00 8.00 5.984 688 atgctagctaggcgatgctagct 409 23 0 52.17 62.989 9.00 8.00 5.989 689 actgatcgatcatcatgctagctac 268 25 0 44.00 60.996 10.00 4.00 5.996 690 agtgatgcatgctagtagtgatgt 27 24 0 41.67 57.997 6.00 3.00 6.003 691 tagctagctactgatcgatgctacat 301 26 0 42.31 60.010 10.00 4.00 6.010 692 ctactagctagctgactgatacgcg 461 25 0 52.00 61.048 8.00 4.00 6.048 693 atcggattagctagctgatgctagt 5 25 0 44.00 61.051 10.00 8.00 6.051 694 ctgatcatcatcgatgctagctagt 516 25 0 44.00 61.106 8.00 6.00 6.106 695 actagctagctgatcatcatcgatg 507 25 0 44.00 61.106 8.00 8.00 6.106 696 ctgatcgatcatcatgctagctact 269 25 0 44.00 61.106 10.00 2.00 6.106 697 gattagctagctgatgctagtgatg 9 25 0 44.00 58.837 10.00 5.00 6.163 698 tagctatttagctagctgactgatcg 250 26 0 42.31 59.800 10.00 4.00 6.200 699 tctagctagctgactagctgatcga 587 25 0 48.00 61.254 9.00 5.00 6.254 700 gatcatcatcgatgctagctagta 518 24 0 41.67 57.735 8.00 8.00 6.265 701 gatcgatcatcatgctagctacta 271 24 0 41.67 57.735 8.00 4.00 6.265 702 atgctactagctagctgactgatacg 458 26 0 46.15 59.732 8.00 2.00 6.268 703 tgctagctagctgatcgatcgtag 557 24 0 50.00 62.269 10.00 8.00 6.269 704 ctagctactatcatctctgcgcga 352 24 0 50.00 62.269 6.00 4.00 6.269 705 ctagctagctgactgatcgatcga 105 24 0 50.00 62.323 8.00 8.00 6.323 706 tgctagtgatgcatgctagtagtga 23 25 0 44.00 61.324 8.00 5.00 6.324 707 gatcatcatcgatgctagctagtag 518 25 0 44.00 58.669 8.00 6.00 6.331 708 tactagctagctgatcatcatcgat 506 25 0 40.00 58.669 8.00 6.00 6.331 709 gatcgatcatcatgctagctactag 271 25 0 44.00 58.669 8.00 6.00 6.331 710 tagctgactagctgatcgatcgtag 593 25 0 48.00 61.355 8.00 8.00 6.355 711 ctagctgactagctgatcgatcgta 592 25 0 48.00 61.355 8.00 6.00 6.355 712 atgcatgctactagctagctgactg 454 25 0 48.00 61.403 8.00 6.00 6.403 713 catgcatgctactagctagctgact 453 25 0 48.00 61.403 8.00 6.00 6.403 714 tagctagctactatcatcgatcga 427 24 0 41.67 57.596 10.00 8.00 6.404 715 ctactagctagctgatcatcatcg 505 24 0 45.83 57.570 8.00 5.00 6.430 716 ctgatcatcgatgctactagctag 195 24 0 45.83 57.570 8.00 8.00 6.430 717 tgctactagctagctgatcatcat 206 24 0 41.67 57.568 8.00 5.00 6.432 718 atgctactagctagctgatcatca 205 24 0 41.67 57.568 8.00 7.00 6.432 719 agctagctagctactgatcgatgct 298 25 0 48.00 61.440 12.00 4.00 6.440 720 atgctactagctagctgatcatcatc 205 26 0 42.31 59.532 8.00 7.00 6.468 721 ctactagctagctgatcatcatcgat 505 26 0 42.31 59.527 8.00 6.00 6.473 722 tgctagctagtagctatcgatcatc 530 25 0 44.00 58.507 11.00 5.00 6.493 723 tgctagctagctactatcatcgatc 424 25 0 44.00 58.507 10.00 4.00 6.493 724 ctagctagctactatcatcgatcga 426 25 0 44.00 58.506 10.00 8.00 6.494 725 gatgcatgcatgctactagctagc 449 24 0 50.00 62.511 10.00 8.00 6.511 726 ctagctagctgatcgatcgatgtg 70 24 0 50.00 62.543 10.00 8.00 6.543 727 tagctagctactatcatctctgcg 349 24 0 45.83 57.434 10.00 2.00 6.566 728 ctagctagctactgatcgatgcta 300 24 0 45.83 57.434 10.00 6.00 6.566 729 ctagctactgatcgatgctacatc 304 24 0 45.83 57.431 6.00 6.00 6.569 730 ctagctactgatcgatgctacatcg 304 25 0 48.00 61.570 8.00 8.00 6.570 731 ctagctagctactgatcgatgctaca 300 26 0 46.15 60.576 10.00 2.00 6.576 732 gcatgctactagctagctgactgata 456 26 0 46.15 60.584 8.00 4.00 6.584 733 tcgatcgatgctagtatgctagcta 326 25 0 44.00 61.618 10.00 8.00 6.618 734 atgctagtgatgcatgctagtagt 22 24 0 41.67 57.372 8.00 6.00 6.628 735 tatttagctagctgactgatcgatc 254 25 0 40.00 58.360 8.00 8.00 6.640 736 ctagctagctactatcatctctgcg 348 25 0 48.00 58.347 10.00 2.00 6.653 737 tagctagctgatgctagtgatgcat 12 25 0 44.00 61.696 8.00 6.00 6.696 738 tagctagctactgatcgatgctac 301 24 0 45.83 57.298 10.00 4.00 6.702 739 ctatttagctagctgactgatcga 253 24 0 41.67 57.276 8.00 4.00 6.724 740 gctatttagctagctgactgatcgat 252 26 0 42.31 60.726 8.00 6.00 6.726 741 catgctactagctagctgactgat 457 24 0 45.83 57.265 8.00 4.00 6.735 742 atcgatcatcgatgctagctagct 545 24 0 45.83 62.761 11.00 8.00 6.761 743 ctagctagctactgatcgatgctac 300 25 0 48.00 58.222 10.00 4.00 6.778 744 tagtgatgcatgctagtagtgatg 26 24 0 41.67 57.220 6.00 5.00 6.780 745 gatgcatgctagtagtgatgtatacg 30 26 0 42.31 59.217 7.00 4.00 6.783 746 ctatttagctagctgactgatcgatc 253 26 0 42.31 59.213 8.00 8.00 6.787 747 ctagtgatgcatgctagtagtgatg 25 25 0 44.00 58.212 8.00 5.00 6.788 748 tgcatgctagtagtgatgtatacg 32 24 0 41.67 57.201 6.00 4.00 6.799 749 gctaggcgatgctagctagctacta 415 25 0 52.00 61.803 10.00 8.00 6.803 750 tagctaggcgatgctagctagctac 413 25 0 52.00 61.803 10.00 8.00 6.803 751 tactagctagctgactgatacgcga 462 25 0 48.00 61.817 8.00 3.00 6.817 752 atcgatcgatgctagtatgctagct 325 25 0 44.00 61.817 10.00 8.00 6.817 753 attagctagctgatgctagtgatg 10 24 0 41.67 57.164 10.00 4.00 6.836 754 gatcgatgctagtatgctagctagct 328 26 0 46.15 60.851 10.00 8.00 6.851 755 gctgatcatcatcgatgctagcta 515 24 0 45.83 62.867 8.00 6.00 6.867 756 tagctgatcatcatcgatgctagc 513 24 0 45.83 62.867 8.00 8.00 6.867 757 gctagctgatcatcatcgatgcta 511 24 0 45.83 62.867 8.00 8.00 6.867 758 tagctagctgatcatcatcgatgc 509 24 0 45.83 62.867 8.00 7.00 6.867 759 actgatacgcgatgctagctagct 475 24 0 50.00 62.905 10.00 8.00 6.905 760 gactgatcgatcatcatgctagcta 267 25 0 44.00 61.916 10.00 6.00 6.916 761 agtgatgcatgctagtagtgatgta 27 25 0 40.00 58.077 6.00 5.00 6.923 762 tagtgatgcatgctagtagtgatgt 26 25 0 40.00 58.077 6.00 3.00 6.923 763 gtgatgcatgctagtagtgatgta 28 24 0 41.67 57.072 6.00 5.00 6.928 764 atttagctagctgactgatcgatcat 255 26 0 38.46 60.936 10.00 8.00 6.936 765 tacatcgatcgatgctagtatgctag 322 26 0 42.31 60.954 12.00 8.00 6.954 766 tgcatgctagtagtgatgtatacgt 32 25 0 40.00 58.033 6.00 6.00 6.967 767 ctactatcatcgatcgatgcatgc 434 24 0 45.83 62.969 12.00 8.00 6.969 768 ctactagctagctgactgatacgc 461 24 0 50.00 57.004 8.00 3.00 6.996 769 gctactagctagctgactgatacg 460 24 0 50.00 57.004 8.00 2.00 6.996 770 tgactgatacgcgatgctagctag 473 24 0 50.00 62.998 8.00 8.00 6.998 771 ctgactgatacgcgatgctagcta 472 24 0 50.00 62.998 6.00 6.00 6.998 772 tagctgactgatacgcgatgctag 469 24 0 50.00 62.998 4.00 4.00 6.998 773 ctagctgactgatacgcgatgcta 468 24 0 50.00 62.998 4.00 2.00 6.998 774 tagctagctgactgatacgcgatg 465 24 0 50.00 62.998 8.00 2.00 6.998 775 ctagtgatgcatgctagtagtgatgt 25 26 0 42.31 58.999 8.00 3.00 7.001 776 gctgatcatcatctagctagtagcttt 153 27 0 40.74 60.007 9.00 5.00 7.007 777 actagctagctgactgatacgcgat 463 25 0 48.00 62.015 8.00 4.00 7.015 778 atcgatgctactagctagctgatcat 201 26 0 42.31 61.064 8.00 8.00 7.064 779 gatgcatgctagtagtgatgtatacgt 30 27 0 40.74 59.925 7.00 6.00 7.075 780 atgctactagctagctgatcatcat 205 25 0 40.00 57.922 8.00 8.00 7.078 781 tgatcatcatcgatgctagctagtag 517 26 0 42.31 61.079 8.00 6.00 7.079 782 ctgatcatcatcgatgctagctagta 516 26 0 42.31 61.079 8.00 8.00 7.079 783 tactagctagctgatcatcatcgatg 506 26 0 42.31 61.079 8.00 8.00 7.079 784 tgatcgatcatcatgctagctactag 270 26 0 42.31 61.079 10.00 6.00 7.079 785 ctgatcgatcatcatgctagctacta 269 26 0 42.31 61.079 10.00 4.00 7.079 786 gctgatcatcatctagctagtagctt 153 26 0 42.31 58.900 9.00 7.00 7.100 787 atgctagctagtagctatcgatcatc 529 26 0 42.31 58.799 11.00 5.00 7.201 788 atgctagctagctactatcatcgatc 423 26 0 42.31 58.799 12.00 4.00 7.201 789 gcatgctagtagtgatgtatacgtagc 33 27 0 44.44 59.740 6.00 6.00 7.260 790 agctatttagctagctgactgatcga 251 26 0 42.31 61.281 8.00 4.00 7.281 791 agctgatcatcatctagctagtagctt 152 27 0 40.74 59.708 10.00 7.00 7.292 792 gctagctagctactgatcgatgctac 299 26 0 50.00 61.306 10.00 4.00 7.306 793 agctactagctagctgatcatcatc 503 25 0 44.00 57.687 10.00 7.00 7.313 794 agctgatcatcatctagctagtagc 152 25 0 44.00 57.687 10.00 7.00 7.313 795 gactgatcgatcgatgctagctagt 115 25 0 48.00 62.355 10.00 6.00 7.355 796 ctatttagctagctgactgatcgat 253 25 0 40.00 57.634 8.00 6.00 7.366 797 atgctagctagctgatcgatcgtag 556 25 0 48.00 62.397 10.00 8.00 7.397 798 ctagctactatcatctctgcgcgat 352 25 0 48.00 62.397 6.00 4.00 7.397 799 ctagctagctactatcatctctgcgc 348 26 0 50.00 61.404 10.00 4.00 7.404 800 gctagctagctactatcatctctgcg 347 26 0 50.00 61.404 10.00 2.00 7.404 801 agctagctagctactgatcgatgcta 298 26 0 46.15 61.404 12.00 6.00 7.404 802 atgcatgctagtagtgatgtatacg 31 25 0 40.00 57.577 7.00 4.00 7.423 803 ctagctagctgactagctgatcgatc 588 26 0 50.00 61.427 9.00 8.00 7.427 804 tctagctagctgactagctgatcgat 587 26 0 46.15 61.427 9.00 6.00 7.427 805 atctagctagctgactagctgatcga 586 26 0 46.15 61.427 11.00 5.00 7.427 806 ctagctagctagctgactgatcgatc 101 26 0 50.00 61.427 12.00 8.00 7.427 807 gctagctagctactatcatctctgc 347 25 0 48.00 57.554 10.00 2.00 7.446 808 gctagtgatgcatgctagtagtgatg 24 26 0 46.15 61.500 8.00 5.00 7.500 809 tgctagtgatgcatgctagtagtgat 23 26 0 42.31 61.500 8.00 4.00 7.500 810 atgctagtgatgcatgctagtagtga 22 26 0 42.31 61.500 8.00 4.00 7.500 811 gatgctagtgatgcatgctagtagtg 21 26 0 46.15 61.500 8.00 4.00 7.500 812 tgatgctagtgatgcatgctagtagt 20 26 0 42.31 61.500 8.00 6.00 7.500 813 actgatcgatcgatgctagctagtag 116 26 0 46.15 61.522 10.00 6.00 7.522 814 gtgatgcatgctagtagtgatgtat 28 25 0 40.00 57.469 7.00 7.00 7.531 815 agctagctactgatcgatgctacatc 302 26 0 46.15 61.533 8.00 6.00 7.533 816 agctaggcgatgctagctagctact 414 25 0 52.00 62.538 10.00 6.00 7.538 817 ctagctatttagctagctgactgatcg 249 27 0 44.44 60.549 12.00 6.00 7.549 818 gctagctagctactatcatcgatcga 425 26 0 46.15 61.570 10.00 8.00 7.570 819 agctagctagctactatcatctctgc 346 26 0 46.15 58.428 12.00 2.00 7.572 820 agtgatgcatgctagtagtgatgtat 27 26 0 38.46 58.410 7.00 7.00 7.590 821 ctgatgctagtgatgcatgctagtag 19 26 0 46.15 61.604 8.00 6.00 7.604 822 ttagctagctgatgctagtgatgca 11 25 0 44.00 62.607 8.00 4.00 7.607 823 agctactagctagctgatcatcatcg 503 26 0 46.15 61.633 10.00 5.00 7.633 824 ctgatcatcgatgctactagctagct 195 26 0 46.15 61.633 8.00 8.00 7.633 825 agctgatcatcgatgctactagctag 193 26 0 46.15 61.633 10.00 8.00 7.633 826 ctagctagctgatcatcgatgctact 187 26 0 46.15 61.633 10.00 7.00 7.633 827 catgctactagctagctgactgata 457 25 0 44.00 57.367 8.00 4.00 7.633 828 atgcatgctagtagtgatgtatacgt 31 26 0 38.46 58.359 7.00 6.00 7.641 829 catcggattagctagctgatgctag 4 25 0 48.00 62.679 10.00 8.00 7.679 830 tatcgatcatcgatgctagctagct 544 25 0 44.00 62.682 11.00 8.00 7.682 831 gctgatcatcatctactatcatcatca 218 27 0 37.04 60.705 8.00 8.00 7.705 832 atcgatcgatgctagtatgctagcta 325 26 0 42.31 61.770 10.00 8.00 7.770 833 ctagctagctactgatcgatgctacat 300 27 0 44.44 60.771 10.00 4.00 7.771 834 atgctagctagtagctatcgatcat 529 25 0 40.00 57.227 11.00 8.00 7.773 835 atgctagctagctactatcatcgat 423 25 0 40.00 57.227 12.00 6.00 7.773 836 actgatcgatcatcatgctagctact 268 26 0 42.31 61.773 10.00 3.00 7.773 837 gctgatcatcatctactatcatcatc 218 26 0 38.46 58.224 7.00 6.00 7.776 838 tgcatgctactagctagctgactga 455 25 0 48.00 62.811 8.00 4.00 7.811 839 gatcatcatcgatgctagctagtagc 518 26 0 46.15 61.816 8.00 8.00 7.816 840 gatcgatcatcatgctagctactagc 271 26 0 46.15 61.816 8.00 8.00 7.816 841 ggattagctagctgatgctagtgatg 8 26 0 46.15 61.822 10.00 5.00 7.822 842 catgctactagctagctgactgatac 457 26 0 46.15 58.154 8.00 2.00 7.846 843 tagtgatgcatgctagtagtgatgta 26 26 0 38.46 58.152 6.00 5.00 7.848 844 agctgatcatcatctactatcatcatc 217 27 0 37.04 59.122 9.00 6.00 7.878 845 tgcatgctagtagtgatgtatacgta 32 26 0 38.46 58.108 6.00 6.00 7.892 846 tatttagctagctgactgatcgatcat 254 27 0 37.04 60.916 10.00 8.00 7.916 847 agctgatcatcatctactatcatca 217 25 0 36.00 57.081 9.00 6.00 7.919 848 gattagctagctgatgctagtgatgc 9 26 0 46.15 61.942 10.00 5.00 7.942 849 ctagtgatgcatgctagtagtgatgta 25 27 0 40.74 59.041 8.00 5.00 7.959 850 tactagctagctgactgatacgcgat 462 26 0 46.15 61.961 8.00 4.00 7.961 851 tgcatgctagtagtgatgtatacgtag 32 27 0 40.74 58.973 6.00 4.00 8.027 852 gctagctgatcatcatctactatcatc 214 27 0 40.74 58.951 11.00 4.00 8.049 853 agctagctgatcatcatctactatca 213 26 0 38.46 57.910 11.00 6.00 8.090 854 ctagctgactagctgatcgatcgtag 592 26 0 50.00 62.099 8.00 8.00 8.099 855 tagctactagctagctgatcatcatc 502 26 0 42.31 57.771 11.00 7.00 8.229 856 tagctgatcatcatctagctagtagc 151 26 0 42.31 57.771 12.00 7.00 8.229 857 gcatgctactagctagctgactgatac 456 27 0 48.15 61.231 8.00 4.00 8.231 858 tagctatttagctagctgactgatcga 250 27 0 40.74 61.251 10.00 6.00 8.251 859 gactgatcgatcgatgctagctagta 115 26 0 46.15 62.288 10.00 8.00 8.288 860 tgctagctagtagctatcgatcatcg 530 26 0 46.15 62.331 11.00 7.00 8.331 861 tgctagctagctactatcatcgatcg 424 26 0 46.15 62.331 10.00 6.00 8.331 862 tcgatcgatgctagtatgctagctag 326 26 0 46.15 62.331 10.00 8.00 8.331 863 gctactagctagctgatcatcatcga 504 26 0 46.15 62.397 8.00 7.00 8.397 864 tcatcgatgctactagctagctgatc 199 26 0 46.15 62.397 9.00 8.00 8.397 865 tgatgcatgctagtagtgatgtatac 29 26 0 38.46 57.564 7.00 6.00 8.436 866 gtgatgcatgctagtagtgatgtata 28 26 0 38.46 57.564 7.00 5.00 8.436 867 agctatttagctagctgactgatcgat 251 27 0 40.74 61.441 8.00 6.00 8.441 868 agctaggcgatgctagctagctacta 414 26 0 50.00 62.471 10.00 8.00 8.471 869 tagctaggcgatgctagctagctact 413 26 0 50.00 62.471 10.00 6.00 8.471 870 ctagctaggcgatgctagctagctac 412 26 0 53.85 62.471 12.00 8.00 8.471 871 tagctagctactgatcgatgctacatc 301 27 0 44.44 61.494 10.00 6.00 8.494 872 agtgatgcatgctagtagtgatgtata 27 27 0 37.04 58.471 7.00 5.00 8.529 873 tagtgatgcatgctagtagtgatgtat 26 27 0 37.04 58.471 7.00 7.00 8.529 874 ctactagctagctgactgatacgcga 461 26 0 50.00 62.530 8.00 3.00 8.530 875 agctgatcatcatctactatcatcat 217 26 0 34.62 57.466 9.00 4.00 8.534 876 tgctactagctagctgactgatacgc 459 26 0 50.00 62.543 8.00 4.00 8.543 877 tgatcatcatctactatcatcatcatc 220 27 0 33.33 58.451 8.00 7.00 8.549 878 tgatgcatgctagtagtgatgtatacg 29 27 0 40.74 61.557 7.00 4.00 8.557 879 gactgatcgatcatcatgctagctac 267 26 0 46.15 62.566 10.00 4.00 8.566 880 atgcatgctagtagtgatgtatacgta 31 27 0 37.04 58.419 7.00 6.00 8.581 881 tcatctagctagtagctttatgctagc 161 27 0 40.74 58.416 11.00 6.00 8.584 882 atctagctagctgactagctgatcgat 586 27 0 44.44 61.585 11.00 7.00 8.585 883 tagctactagctagctgatcatcatcg 502 27 0 44.44 61.591 11.00 5.00 8.591 884 tagctgatcatcgatgctactagctag 192 27 0 44.44 61.591 12.00 8.00 8.591 885 gtgatgcatgctagtagtgatgtatac 28 27 0 40.74 58.348 7.00 7.00 8.652 886 atgctagtgatgcatgctagtagtgat 22 27 0 40.74 61.661 8.00 5.00 8.661 887 gctagctgatcatcatctactatcat 214 26 0 38.46 57.336 11.00 3.00 8.664 888 ctgatcatcatctactatcatcatca 219 26 0 34.62 57.322 8.00 8.00 8.678 889 ttagctagctgatgctagtgatgcat 11 26 0 42.31 62.718 8.00 6.00 8.718 890 attagctagctgatgctagtgatgca 10 26 0 42.31 62.718 10.00 4.00 8.718 891 actgatcgatcatcatgctagctacta 268 27 0 40.74 61.726 10.00 4.00 8.726 892 agctagctgatcatcatctactatcat 213 27 0 37.04 58.233 11.00 6.00 8.767 893 tagctgatcatcatctactatcatca 216 26 0 34.62 57.191 11.00 6.00 8.809 894 ctgatcatcatcgatgctagctagtag 516 27 0 44.44 61.823 8.00 6.00 8.823 895 ctactagctagctgatcatcatcgatg 505 27 0 44.44 61.823 8.00 8.00 8.823 896 ctgatcgatcatcatgctagctactag 269 27 0 44.44 61.823 10.00 6.00 8.823 897 ctagctgatcatcatctactatcatca 215 27 0 37.04 58.131 11.00 6.00 8.869 898 tgcatgctactagctagctgactgat 455 26 0 46.15 62.915 8.00 4.00 8.915 899 atgcatgctactagctagctgactga 454 26 0 46.15 62.915 8.00 4.00 8.915 900 catgctactagctagctgactgatacg 457 27 0 48.15 61.978 8.00 2.00 8.978 901 agctagctagctactgatcgatgctac 298 27 0 48.15 62.004 12.00 4.00 9.004 902 agctagctagctactatcatctctgcg 346 27 0 48.15 62.095 12.00 2.00 9.095 903 gctagtgatgcatgctagtagtgatgt 24 27 0 44.44 62.139 8.00 3.00 9.139 904 gctatttagctagctgactgatcgatc 252 27 0 44.44 62.155 8.00 8.00 9.155 905 ctgatgctagtgatgcatgctagtagt 19 27 0 44.44 62.235 8.00 6.00 9.235 906 catctagctagtagctttatgctagct 162 27 0 40.74 57.761 11.00 8.00 9.239 907 ctgatcatcatctactatcatcatcat 219 27 0 33.33 57.694 8.00 6.00 9.306 908 tagctaggcgatgctagctagctacta 413 27 0 48.15 62.409 10.00 8.00 9.409 909 tagctgatcatcatctactatcatcat 216 27 0 33.33 57.559 11.00 4.00 9.441 910 actagctatttagctagctgactgatc 248 27 0 40.74 57.556 12.00 6.00 9.444 911 atgctagctagtagctatcgatcatcg 529 27 0 44.44 62.447 11.00 7.00 9.447 912 atgctagctagctactatcatcgatcg 423 27 0 44.44 62.447 12.00 6.00 9.447 913 atcgatcgatgctagtatgctagctag 325 27 0 44.44 62.447 10.00 8.00 9.447 914 catgctagtagtgatgtatacgtagct 34 27 0 40.74 57.502 6.00 6.00 9.498 915 gctactagctagctgatcatcatcgat 504 27 0 44.44 62.514 8.00 6.00 9.514 916 atcatcgatgctactagctagctgatc 198 27 0 44.44 62.514 11.00 8.00 9.514 917 ctactagctagctgactgatacgcgat 461 27 0 48.15 62.638 8.00 4.00 9.638 918 atgctactagctagctgactgatacgc 458 27 0 48.15 62.652 8.00 4.00 9.652 919 attagctagctgatgctagtgatgcat 10 27 0 40.74 62.819 10.00 6.00 9.819 920 tgcatgctactagctagctgactgata 455 27 0 44.44 62.833 8.00 4.00 9.833 921 tctagctagctgactagctgatcgatc 587 27 0 48.15 62.883 9.00 8.00 9.883 922 gactgatcgatcgatgctagctagtag 115 27 0 48.15 62.974 10.00 6.00 9.974 923 ctgatcatcatctagctagtagcttta 154 27 0 37.04 57.019 9.00 7.00 9.981 primer3-2.3.6/test/primer_list_last/SP01-06-with-repeat1.for0000664000077300007730000001074612231156244022346 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self lib qual- # sequence start ln N GC% Tm any end sim lity 0 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 10.00 2.909 1 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 10.00 3.033 2 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 10.00 3.626 3 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 10.00 4.023 4 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 10.00 4.199 5 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 10.00 4.207 6 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 10.00 4.458 7 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 10.00 4.533 8 GAATCGCAATCAAATTTTTTC 33 21 0 28.57 56.457 6.00 2.00 9.00 4.543 9 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 10.00 4.596 10 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 10.00 4.923 11 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 10.00 5.202 12 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 10.00 5.202 13 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 10.00 5.202 14 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 10.00 5.202 15 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 10.00 5.211 16 AATCGCAATCAAATTTTTTCT 34 21 0 23.81 55.693 6.00 2.00 10.00 5.307 17 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 10.00 5.323 18 AATCGCAATCAAATTTTTTC 34 20 0 25.00 54.571 6.00 2.00 9.00 5.429 19 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 9.00 5.439 20 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 10.00 5.502 21 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 10.00 5.544 22 ATCGCAATCAAATTTTTTCT 35 20 0 25.00 54.168 6.00 2.00 10.00 5.832 23 AATCGCAATCAAATTTTTTCTA 34 22 0 22.73 55.850 6.00 2.00 9.00 6.150 24 TCGCAATCAAATTTTTTCTA 36 20 0 25.00 53.814 6.00 2.00 9.00 6.186 25 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 10.00 6.204 26 TCGCAATCAAATTTTTTCTAT 36 21 0 23.81 54.389 6.00 2.00 9.00 6.611 27 ATCGCAATCAAATTTTTTCTA 35 21 0 23.81 54.389 6.00 2.00 9.00 6.611 28 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 10.00 6.694 29 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 10.00 6.843 30 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 10.00 7.226 31 TCGCAATCAAATTTTTTCT 36 19 0 26.32 53.559 6.00 2.00 10.00 7.441 32 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 11.00 7.463 33 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 10.00 7.653 34 ATCGCAATCAAATTTTTTC 35 19 0 26.32 52.911 6.00 2.00 9.00 8.089 35 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 10.00 8.199 36 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 10.00 8.382 37 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 10.00 8.397 38 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 11.00 8.574 39 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 10.00 8.652 40 TCGCAATCAAATTTTTTCTATGACCT 36 26 0 30.77 63.123 6.00 1.00 10.00 9.123 41 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 10.00 9.235 42 AATTATGAATCGCAATCAA 27 19 0 26.32 51.609 5.00 2.00 10.00 9.391 43 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 10.00 9.507 44 TCGCAATCAAATTTTTTC 36 18 0 27.78 52.197 6.00 2.00 9.00 9.803 45 ATCGCAATCAAATTTTTTCTATGACCT 35 27 0 29.63 63.204 6.00 1.00 10.00 10.204 46 AATCGCAATCAAATTTTTTCTATGACC 34 27 0 29.63 63.467 6.00 0.00 11.00 10.467 47 TGAATCGCAATCAAATTTTTTCTATGA 32 27 0 25.93 63.763 6.00 2.00 11.00 10.763 48 TCGCAATCAAATTTTTTCTATGACCTG 36 27 0 33.33 65.188 6.00 1.00 9.00 12.188 49 TATGAATCGCAATCAAAT 30 18 0 27.78 49.586 4.00 2.00 10.00 12.414 50 ATTATGAATCGCAATCAA 28 18 0 27.78 49.586 5.00 2.00 10.00 12.414 primer3-2.3.6/test/primer_list_last/included_test_w_middle.for0000664000077300007730000005577012231156244023630 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGTGTCTTGTGGGTGTGTCTG 78 21 0 52.38 60.680 1.00 1.00 1.680 1 TGTCTTGTGGGTGTGTCTGTT 80 21 0 47.62 59.059 1.00 0.00 1.941 2 GTGTCTTGTGGGTGTGTCTGTT 79 22 0 50.00 59.972 1.00 0.00 2.028 3 GAACATGTGTGGCATTGTGT 20 20 0 45.00 57.775 6.00 0.00 2.225 4 GTGTCTTGTGGGTGTGTCTGT 79 21 0 52.38 58.472 1.00 0.00 2.528 5 TGTCTTGTGGGTGTGTCTGT 80 20 0 50.00 57.431 1.00 0.00 2.569 6 GTGTCTTGTGGGTGTGTCTG 79 20 0 55.00 57.431 1.00 1.00 2.569 7 TGTGTCTTGTGGGTGTGTCT 78 20 0 50.00 57.431 1.00 0.00 2.569 8 TGTCTTGTGGGTGTGTCTGTTA 80 22 0 45.45 59.108 2.00 2.00 2.892 9 TTGTGGGTGTGTCTGTTAAACA 84 22 0 40.91 58.997 7.00 7.00 3.003 10 GTGTCTTGTGGGTGTGTCTGTTA 79 23 0 47.83 59.984 2.00 2.00 3.016 11 CTTGTGGGTGTGTCTGTTAAACA 83 23 0 43.48 59.981 7.00 7.00 3.019 12 GAACATGTGTGGCATTGTGTA 20 21 0 42.86 57.880 6.00 2.00 3.120 13 TGTCTTGTGGGTGTGTCTGTTAA 80 23 0 43.48 60.493 4.00 4.00 3.493 14 TGTGGGTGTGTCTGTTAAACA 85 21 0 42.86 57.489 7.00 7.00 3.511 15 TGTGTCTTGTGGGTGTGTCTGT 78 22 0 50.00 61.549 1.00 0.00 3.549 16 AAACATGTGTGGTATTGTGCA 101 21 0 38.10 57.391 6.00 4.00 3.609 17 TTGTGGGTGTGTCTGTTAAACAT 84 23 0 39.13 59.319 7.00 5.00 3.681 18 GAACATGTGTGGCATTGTGTAT 20 22 0 40.91 58.283 6.00 2.00 3.717 19 TGGGTGTGTCTGTTAAACATGTG 87 23 0 43.48 60.746 7.00 4.00 3.746 20 TGTGGGTGTGTCTGTTAAACATG 85 23 0 43.48 60.746 7.00 5.00 3.746 21 TCTTGTGGGTGTGTCTGTTAAA 82 22 0 40.91 58.187 4.00 3.00 3.813 22 AGCTTCTTGACTGTGTGTTGTG 148 22 0 45.45 58.075 4.00 0.00 3.925 23 TCTTGTGGGTGTGTCTGTTAAAC 82 23 0 43.48 59.063 5.00 5.00 3.937 24 GTCTTGTGGGTGTGTCTGTTAAA 81 23 0 43.48 59.063 4.00 3.00 3.937 25 TGGATGTAGCTTCTTGACTGTG 141 22 0 45.45 58.036 4.00 1.00 3.964 26 AGCTTCTTGACTGTGTGTTGTGT 148 23 0 43.48 58.981 4.00 0.00 4.019 27 GGATGTAGCTTCTTGACTGTGTG 142 23 0 47.83 58.925 4.00 0.00 4.075 28 TGGATGTAGCTTCTTGACTGTGT 141 23 0 43.48 58.925 4.00 1.00 4.075 29 TTAAACATGTGTGGTATTGTGCA 99 23 0 34.78 58.925 6.00 4.00 4.075 30 GGGTGTGTCTGTTAAACATGTG 88 22 0 45.45 57.912 7.00 4.00 4.088 31 TGGGTGTGTCTGTTAAACATGT 87 22 0 40.91 57.912 7.00 6.00 4.088 32 GTGGGTGTGTCTGTTAAACATG 86 22 0 45.45 57.912 7.00 5.00 4.088 33 TGTGGGTGTGTCTGTTAAACAT 85 22 0 40.91 57.912 7.00 5.00 4.088 34 AACATGTGTGGCATTGTGTATACA 21 24 0 37.50 60.103 8.00 8.00 4.103 35 GTCTTGTGGGTGTGTCTGTTAAAC 81 24 0 45.83 59.875 5.00 5.00 4.125 36 CATGTGTGGCATTGTGTATACA 23 22 0 40.91 57.850 8.00 8.00 4.150 37 AAACATGTGTGGTATTGTGCAT 101 22 0 36.36 57.811 6.00 2.00 4.189 38 GGGTGTGTCTGTTAAACATGTGT 88 23 0 43.48 58.808 7.00 6.00 4.192 39 GTGGGTGTGTCTGTTAAACATGT 86 23 0 43.48 58.808 7.00 6.00 4.192 40 ACATGTGTGGCATTGTGTATACA 22 23 0 39.13 58.766 8.00 8.00 4.234 41 CTTGTGGGTGTGTCTGTTAAACAT 83 24 0 41.67 60.239 7.00 5.00 4.239 42 GGATGTAGCTTCTTGACTGTGTGT 142 24 0 45.83 59.748 4.00 0.00 4.252 43 GTTAAACATGTGTGGTATTGTGCA 98 24 0 37.50 59.733 6.00 4.00 4.267 44 TGTTAAACATGTGTGGTATTGTGC 97 24 0 37.50 59.733 7.00 2.00 4.267 45 GTCTTGTGGGTGTGTCTGTTAA 81 22 0 45.45 57.635 4.00 4.00 4.365 46 TAAACATGTGTGGTATTGTGCA 100 22 0 36.36 57.504 6.00 4.00 4.496 47 TGTGTGTTGTGTATGGATGTAGC 128 23 0 43.48 58.499 2.00 2.00 4.501 48 TGTGTGTTGTGTATGGATGTAGCT 128 24 0 41.67 59.493 4.00 4.00 4.507 49 ATGGATGTAGCTTCTTGACTGTG 140 23 0 43.48 58.408 4.00 1.00 4.592 50 GAACATGTGTGGCATTGTGTATA 20 23 0 39.13 58.360 6.00 4.00 4.640 51 TTGTGCATATACATGTGTGTTGTG 115 24 0 37.50 59.345 6.00 2.00 4.655 52 ATGGATGTAGCTTCTTGACTGTGT 140 24 0 41.67 59.238 4.00 1.00 4.762 53 TTAAACATGTGTGGTATTGTGCAT 99 24 0 33.33 59.233 6.00 2.00 4.767 54 GAACATGTGTGGCATTGTGTATAC 20 24 0 41.67 59.208 6.00 6.00 4.792 55 CTTGTGGGTGTGTCTGTTAAAC 83 22 0 45.45 57.176 5.00 5.00 4.824 56 TAGCTTCTTGACTGTGTGTTGTG 147 23 0 43.48 58.160 4.00 0.00 4.840 57 TGTAGCTTCTTGACTGTGTGTTG 145 23 0 43.48 58.160 4.00 0.00 4.840 58 TGTGTTGTGTATGGATGTAGCTTC 130 24 0 41.67 59.091 4.00 4.00 4.909 59 TAGCTTCTTGACTGTGTGTTGTGT 147 24 0 41.67 59.030 4.00 0.00 4.970 60 GTAGCTTCTTGACTGTGTGTTGTG 146 24 0 45.83 59.030 4.00 0.00 4.970 61 TGTAGCTTCTTGACTGTGTGTTGT 145 24 0 41.67 59.030 4.00 0.00 4.970 62 TGTGTCTGTTAAACATGTGTGG 91 22 0 40.91 57.024 7.00 2.00 4.976 63 TGTGTTGTGTATGGATGTAGCTTCT 130 25 0 40.00 60.002 4.00 2.00 5.002 64 GTTAAACATGTGTGGTATTGTGCAT 98 25 0 36.00 59.991 6.00 2.00 5.009 65 TGTGTCTGTTAAACATGTGTGGT 91 23 0 39.13 57.983 7.00 0.00 5.017 66 GTGTGTCTGTTAAACATGTGTGG 90 23 0 43.48 57.983 7.00 2.00 5.017 67 GGTGTGTCTGTTAAACATGTGTG 89 23 0 43.48 57.983 7.00 4.00 5.017 68 TGTGCATATACATGTGTGTTGTG 116 23 0 39.13 57.923 6.00 2.00 5.077 69 AAACATGTGTGGTATTGTGCATA 101 23 0 34.78 57.903 6.00 4.00 5.097 70 TAAACATGTGTGGTATTGTGCAT 100 23 0 34.78 57.903 6.00 2.00 5.097 71 GTGTGTTGTGTATGGATGTAGCTTC 129 25 0 44.00 59.880 4.00 4.00 5.120 72 GTGTGTCTGTTAAACATGTGTGGT 90 24 0 41.67 58.868 7.00 0.00 5.132 73 TTGTGCATATACATGTGTGTTGTGT 115 25 0 36.00 60.151 6.00 0.00 5.151 74 ATGTGTGTTGTGTATGGATGTAGC 127 24 0 41.67 58.842 2.00 2.00 5.158 75 GTAGCTTCTTGACTGTGTGTTGTGT 146 25 0 44.00 59.835 4.00 0.00 5.165 76 TTGTGTATGGATGTAGCTTCTTGA 134 24 0 37.50 58.834 4.00 2.00 5.166 77 TGTGCATATACATGTGTGTTGTGT 116 24 0 37.50 58.828 6.00 0.00 5.172 78 ATGTGTGTTGTGTATGGATGTAGCT 127 25 0 40.00 59.772 4.00 4.00 5.228 79 GTGTCTTGTGGGTGTGTCTGTTAA 79 24 0 45.83 61.270 4.00 4.00 5.270 80 GATGTAGCTTCTTGACTGTGTGTTG 143 25 0 44.00 60.272 4.00 0.00 5.272 81 ACATGTGTGTTGTGTATGGATGT 125 23 0 39.13 57.646 6.00 0.00 5.354 82 ATTGTGCATATACATGTGTGTTGTG 114 25 0 36.00 59.637 6.00 2.00 5.363 83 TTGTGTATGGATGTAGCTTCTTGAC 134 25 0 40.00 59.615 4.00 1.00 5.385 84 GTTGTGTATGGATGTAGCTTCTTGA 133 25 0 40.00 59.615 4.00 2.00 5.385 85 TGTTGTGTATGGATGTAGCTTCTTG 132 25 0 40.00 60.458 4.00 2.00 5.458 86 ATGTAGCTTCTTGACTGTGTGTTG 144 24 0 41.67 58.520 4.00 0.00 5.480 87 GGTGTGTCTGTTAAACATGTGTGG 89 24 0 45.83 61.509 7.00 2.00 5.509 88 GGGTGTGTCTGTTAAACATGTGTG 88 24 0 45.83 61.509 7.00 4.00 5.509 89 TGGGTGTGTCTGTTAAACATGTGT 87 24 0 41.67 61.509 7.00 6.00 5.509 90 GTGGGTGTGTCTGTTAAACATGTG 86 24 0 45.83 61.509 7.00 4.00 5.509 91 TGTGGGTGTGTCTGTTAAACATGT 85 24 0 41.67 61.509 7.00 6.00 5.509 92 TATGGATGTAGCTTCTTGACTGTG 139 24 0 41.67 58.476 4.00 1.00 5.524 93 TGTATGGATGTAGCTTCTTGACTG 137 24 0 41.67 58.476 4.00 1.00 5.524 94 TGTGTATGGATGTAGCTTCTTGA 135 23 0 39.13 57.467 4.00 2.00 5.533 95 CTGTTAAACATGTGTGGTATTGTGC 96 25 0 40.00 60.599 7.00 2.00 5.599 96 TGGATGTAGCTTCTTGACTGTGTG 141 24 0 45.83 61.619 4.00 0.00 5.619 97 AACATGTGTGGCATTGTGTATAC 21 23 0 39.13 57.373 6.00 6.00 5.627 98 TGTGTATGGATGTAGCTTCTTGAC 135 24 0 41.67 58.339 4.00 1.00 5.661 99 ATGTAGCTTCTTGACTGTGTGTTGT 144 25 0 40.00 59.333 4.00 0.00 5.667 100 TGGTATTGTGCATATACATGTGTG 110 24 0 37.50 58.315 6.00 4.00 5.685 101 TGTGGTATTGTGCATATACATGTG 108 24 0 37.50 58.315 6.00 4.00 5.685 102 TGTGTGGTATTGTGCATATACATG 106 24 0 37.50 58.315 6.00 6.00 5.685 103 CATGTGTGGTATTGTGCATATACA 104 24 0 37.50 58.315 6.00 6.00 5.685 104 TATGGATGTAGCTTCTTGACTGTGT 139 25 0 40.00 59.274 4.00 1.00 5.726 105 GTATGGATGTAGCTTCTTGACTGTG 138 25 0 44.00 59.274 4.00 1.00 5.726 106 TGTATGGATGTAGCTTCTTGACTGT 137 25 0 40.00 59.274 4.00 3.00 5.726 107 GTGTATGGATGTAGCTTCTTGACTG 136 25 0 44.00 59.274 4.00 1.00 5.726 108 TGTGTATGGATGTAGCTTCTTGACT 135 25 0 40.00 59.274 4.00 1.00 5.726 109 AGCTTCTTGACTGTGTGTTGTGTG 148 24 0 45.83 61.730 4.00 0.00 5.730 110 TTAAACATGTGTGGTATTGTGCATA 99 25 0 32.00 59.268 6.00 4.00 5.732 111 TCTTGTGGGTGTGTCTGTTAAACA 82 24 0 41.67 61.733 7.00 7.00 5.733 112 TGTCTTGTGGGTGTGTCTGTTAAA 80 24 0 41.67 61.733 4.00 3.00 5.733 113 AAACATGTGTGGTATTGTGCATAT 101 24 0 33.33 58.262 6.00 4.00 5.738 114 TGTGTGTTGTGTATGGATGTAGCTT 128 25 0 40.00 60.742 4.00 2.00 5.742 115 TGTGTTGTGTATGGATGTAGCTT 130 23 0 39.13 57.257 4.00 2.00 5.743 116 TGTGTCTTGTGGGTGTGTCTGTT 78 23 0 47.83 62.831 1.00 0.00 5.831 117 GTGTGTTGTGTATGGATGTAGCTT 129 24 0 41.67 58.137 4.00 2.00 5.863 118 GCATATACATGTGTGTTGTGTATGG 119 25 0 40.00 59.136 6.00 3.00 5.864 119 TGGTATTGTGCATATACATGTGTGT 110 25 0 36.00 59.136 6.00 6.00 5.864 120 GTGGTATTGTGCATATACATGTGTG 109 25 0 40.00 59.136 6.00 4.00 5.864 121 TGTGGTATTGTGCATATACATGTGT 108 25 0 36.00 59.136 6.00 6.00 5.864 122 GTGTGGTATTGTGCATATACATGTG 107 25 0 40.00 59.136 6.00 4.00 5.864 123 TGTGTGGTATTGTGCATATACATGT 106 25 0 36.00 59.136 6.00 6.00 5.864 124 ACATGTGTGGTATTGTGCATATACA 103 25 0 36.00 59.136 6.00 6.00 5.864 125 TGTGTCTGTTAAACATGTGTGGTA 91 24 0 37.50 58.069 7.00 2.00 5.931 126 GTTAAACATGTGTGGTATTGTGC 98 23 0 39.13 57.060 6.00 2.00 5.940 127 GGATGTAGCTTCTTGACTGTGTGTT 142 25 0 44.00 60.962 4.00 0.00 5.962 128 TTGTGTATGGATGTAGCTTCTTG 134 23 0 39.13 57.036 4.00 2.00 5.964 129 TTGTGGGTGTGTCTGTTAAACATG 84 24 0 41.67 61.967 7.00 5.00 5.967 130 GTTAAACATGTGTGGTATTGTGCATA 98 26 0 34.62 60.001 6.00 4.00 6.001 131 TAAACATGTGTGGTATTGTGCATA 100 24 0 33.33 57.988 6.00 4.00 6.012 132 GTATGGATGTAGCTTCTTGACTGTGT 138 26 0 42.31 60.018 4.00 1.00 6.018 133 GTGTATGGATGTAGCTTCTTGACTGT 136 26 0 42.31 60.018 4.00 3.00 6.018 134 GTGTGTCTGTTAAACATGTGTGGTA 90 25 0 40.00 58.919 7.00 2.00 6.081 135 TCTGTTAAACATGTGTGGTATTGTG 95 25 0 36.00 58.912 7.00 0.00 6.088 136 TGTCTGTTAAACATGTGTGGTATTG 93 25 0 36.00 58.912 7.00 0.00 6.088 137 GTTGTGTATGGATGTAGCTTCTTG 133 24 0 41.67 57.904 4.00 2.00 6.096 138 TGTTGTGTATGGATGTAGCTTCTT 132 24 0 37.50 57.904 4.00 4.00 6.096 139 GTGGTATTGTGCATATACATGTGTGT 109 26 0 38.46 59.900 6.00 6.00 6.100 140 GTGTGGTATTGTGCATATACATGTGT 107 26 0 38.46 59.900 6.00 6.00 6.100 141 CATGTGTGTTGTGTATGGATGTAG 126 24 0 41.67 57.896 4.00 0.00 6.104 142 TGTGCATATACATGTGTGTTGTGTA 116 25 0 36.00 58.882 6.00 2.00 6.118 143 TTGTGCATATACATGTGTGTTGTGTA 115 26 0 34.62 60.156 6.00 2.00 6.156 144 ACATGTGTGTTGTGTATGGATGTAG 125 25 0 40.00 58.761 6.00 0.00 6.239 145 ACATGTGTGTTGTGTATGGATGTA 125 24 0 37.50 57.747 6.00 2.00 6.253 146 TACATGTGTGTTGTGTATGGATGT 124 24 0 37.50 57.747 6.00 0.00 6.253 147 GTGTTGTGTATGGATGTAGCTTCTT 131 25 0 40.00 58.712 4.00 4.00 6.288 148 GTCTGTTAAACATGTGTGGTATTGTG 94 26 0 38.46 59.680 7.00 0.00 6.320 149 TGTCTGTTAAACATGTGTGGTATTGT 93 26 0 34.62 59.680 7.00 0.00 6.320 150 GTGTCTGTTAAACATGTGTGGTATTG 92 26 0 38.46 59.680 7.00 0.00 6.320 151 TGTGTCTGTTAAACATGTGTGGTATT 91 26 0 34.62 59.680 7.00 1.00 6.320 152 TATTGTGCATATACATGTGTGTTGTG 113 26 0 34.62 59.659 6.00 2.00 6.341 153 GTTGTGTATGGATGTAGCTTCTTGAC 133 26 0 42.31 60.343 4.00 1.00 6.343 154 GGTATTGTGCATATACATGTGTGTTG 111 26 0 38.46 60.346 6.00 4.00 6.346 155 TGGTATTGTGCATATACATGTGTGTT 110 26 0 34.62 60.346 6.00 4.00 6.346 156 AACATGTGTGGTATTGTGCATATACA 102 26 0 34.62 60.346 6.00 6.00 6.346 157 ATGTGTGGTATTGTGCATATACATG 105 25 0 36.00 58.650 6.00 6.00 6.350 158 CATGTGTGGTATTGTGCATATACAT 104 25 0 36.00 58.650 6.00 4.00 6.350 159 GATGTAGCTTCTTGACTGTGTGTT 143 24 0 41.67 57.626 4.00 0.00 6.374 160 ATTGTGCATATACATGTGTGTTGTGT 114 26 0 34.62 60.393 6.00 0.00 6.393 161 TTGTGTATGGATGTAGCTTCTTGACT 134 26 0 38.46 60.455 4.00 1.00 6.455 162 TTAAACATGTGTGGTATTGTGCATAT 99 26 0 30.77 59.536 6.00 4.00 6.464 163 CATGTGTGTTGTGTATGGATGTAGC 126 25 0 44.00 61.472 4.00 2.00 6.472 164 TGTGTCTGTTAAACATGTGTGGTAT 91 25 0 36.00 58.424 7.00 2.00 6.576 165 ATGTGTGGTATTGTGCATATACATGT 105 26 0 34.62 59.420 6.00 6.00 6.580 166 ACATGTGTGGTATTGTGCATATACAT 103 26 0 34.62 59.420 6.00 4.00 6.580 167 GTGTTGTGTATGGATGTAGCTTCT 131 24 0 41.67 57.400 4.00 2.00 6.600 168 TGCATATACATGTGTGTTGTGTATG 118 25 0 36.00 58.378 6.00 5.00 6.622 169 AAACATGTGTGGTATTGTGCATATA 101 25 0 32.00 58.331 6.00 4.00 6.669 170 TAAACATGTGTGGTATTGTGCATAT 100 25 0 32.00 58.331 6.00 4.00 6.669 171 TAGCTTCTTGACTGTGTGTTGTGTG 147 25 0 44.00 61.678 4.00 0.00 6.678 172 TGTAGCTTCTTGACTGTGTGTTGTG 145 25 0 44.00 61.678 4.00 0.00 6.678 173 GTGTGTTGTGTATGGATGTAGCTTCT 129 26 0 42.31 60.727 4.00 2.00 6.727 174 TGTGTCTTGTGGGTGTGTCTGTTA 78 24 0 45.83 62.734 2.00 2.00 6.734 175 ATACATGTGTGTTGTGTATGGATG 123 24 0 37.50 57.241 6.00 0.00 6.759 176 GTGTGTCTGTTAAACATGTGTGGTAT 90 26 0 38.46 59.220 7.00 2.00 6.780 177 ATGGATGTAGCTTCTTGACTGTGTG 140 25 0 44.00 61.791 4.00 0.00 6.791 178 GAACATGTGTGGCATTGTGTATACA 20 25 0 40.00 61.810 8.00 8.00 6.810 179 GTGCATATACATGTGTGTTGTGTATG 117 26 0 38.46 59.190 6.00 5.00 6.810 180 TGTGCATATACATGTGTGTTGTGTAT 116 26 0 34.62 59.190 6.00 3.00 6.810 181 GCATATACATGTGTGTTGTGTATGGA 119 26 0 38.46 60.811 6.00 1.00 6.811 182 CTGTTAAACATGTGTGGTATTGTG 96 24 0 37.50 57.140 7.00 0.00 6.860 183 ATACATGTGTGTTGTGTATGGATGT 123 25 0 36.00 58.124 6.00 0.00 6.876 184 TCTTGTGGGTGTGTCTGTTAAACAT 82 25 0 40.00 61.898 7.00 5.00 6.898 185 AAACATGTGTGGTATTGTGCATATAC 101 26 0 34.62 59.095 6.00 6.00 6.905 186 ATGTGTGTTGTGTATGGATGTAGCTT 127 26 0 38.46 60.948 4.00 2.00 6.948 187 AACATGTGTGGTATTGTGCATATA 102 24 0 33.33 57.002 6.00 4.00 6.998 188 GATGTAGCTTCTTGACTGTGTGTTGT 143 26 0 42.31 61.001 4.00 0.00 7.001 189 CATGTGTGGTATTGTGCATATACATG 104 26 0 38.46 61.147 6.00 6.00 7.147 190 GGTATTGTGCATATACATGTGTGTT 111 25 0 36.00 57.851 6.00 4.00 7.149 191 AACATGTGTGGTATTGTGCATATAC 102 25 0 36.00 57.851 6.00 6.00 7.149 192 GTGTTGTGTATGGATGTAGCTTCTTG 131 26 0 42.31 61.157 4.00 2.00 7.157 193 TGTGTTGTGTATGGATGTAGCTTCTT 130 26 0 38.46 61.157 4.00 4.00 7.157 194 TACATGTGTGTTGTGTATGGATGTA 124 25 0 36.00 57.841 6.00 2.00 7.159 195 TACATGTGTGTTGTGTATGGATGTAG 124 26 0 38.46 58.815 6.00 0.00 7.185 196 GGTGTGTCTGTTAAACATGTGTGGT 89 25 0 44.00 62.217 7.00 0.00 7.217 197 GTGGGTGTGTCTGTTAAACATGTGT 86 25 0 44.00 62.217 7.00 6.00 7.217 198 GTTAAACATGTGTGGTATTGTGCATAT 98 27 0 33.33 60.227 6.00 4.00 7.227 199 CATATACATGTGTGTTGTGTATGGATG 120 27 0 37.04 60.230 6.00 0.00 7.230 200 TGTTAAACATGTGTGGTATTGTGCA 97 25 0 36.00 62.264 7.00 4.00 7.264 201 TGGATGTAGCTTCTTGACTGTGTGT 141 25 0 44.00 62.322 4.00 0.00 7.322 202 TTGTGCATATACATGTGTGTTGTGTAT 115 27 0 33.33 60.389 6.00 3.00 7.389 203 ATTGTGCATATACATGTGTGTTGTGTA 114 27 0 33.33 60.389 6.00 2.00 7.389 204 TATTGTGCATATACATGTGTGTTGTGT 113 27 0 33.33 60.389 6.00 0.00 7.389 205 GTATTGTGCATATACATGTGTGTTGTG 112 27 0 37.04 60.389 6.00 2.00 7.389 206 GTGTCTGTTAAACATGTGTGGTATTGT 92 27 0 37.04 60.396 7.00 0.00 7.396 207 GTGTGTCTGTTAAACATGTGTGGTATT 90 27 0 37.04 60.396 7.00 1.00 7.396 208 TGTTGTGTATAAATGTTGTGTCTTCA 51 26 0 30.77 58.596 4.00 1.00 7.404 209 GTCTTGTGGGTGTGTCTGTTAAACA 81 25 0 44.00 62.427 7.00 7.00 7.427 210 TGTCTTGTGGGTGTGTCTGTTAAAC 80 25 0 44.00 62.427 5.00 5.00 7.427 211 GTGTCTTGTGGGTGTGTCTGTTAAA 79 25 0 44.00 62.427 4.00 3.00 7.427 212 TTAAACATGTGTGGTATTGTGCATATA 99 27 0 29.63 59.560 6.00 4.00 7.440 213 AGCTTCTTGACTGTGTGTTGTGTGT 148 25 0 44.00 62.443 4.00 0.00 7.443 214 AACATGTGTGGTATTGTGCATATACAT 102 27 0 33.33 60.562 6.00 4.00 7.562 215 TAAACATGTGTGGTATTGTGCATATA 100 26 0 30.77 58.395 6.00 4.00 7.605 216 GTGTTGTGTATAAATGTTGTGTCTTCA 50 27 0 33.33 59.358 4.00 1.00 7.642 217 TGTGTTGTGTATAAATGTTGTGTCTTC 49 27 0 33.33 59.358 4.00 0.00 7.642 218 TTGTGGGTGTGTCTGTTAAACATGT 84 25 0 40.00 62.648 7.00 6.00 7.648 219 TATACATGTGTGTTGTGTATGGATG 122 25 0 36.00 57.352 6.00 0.00 7.648 220 CATATACATGTGTGTTGTGTATGGA 120 25 0 36.00 57.352 6.00 0.00 7.648 221 TGCATATACATGTGTGTTGTGTATGG 118 26 0 38.46 61.654 6.00 3.00 7.654 222 TGTGGTATTGTGCATATACATGTGTG 108 26 0 38.46 61.654 6.00 4.00 7.654 223 TGTGTGGTATTGTGCATATACATGTG 106 26 0 38.46 61.654 6.00 4.00 7.654 224 TATGGATGTAGCTTCTTGACTGTGTG 139 26 0 42.31 61.740 4.00 0.00 7.740 225 TGTATGGATGTAGCTTCTTGACTGTG 137 26 0 42.31 61.740 4.00 1.00 7.740 226 TGTGTATGGATGTAGCTTCTTGACTG 135 26 0 42.31 61.740 4.00 1.00 7.740 227 CTTGTGGGTGTGTCTGTTAAACATG 83 25 0 44.00 62.749 7.00 5.00 7.749 228 ATACATGTGTGTTGTGTATGGATGTA 123 26 0 34.62 58.200 6.00 2.00 7.800 229 TATACATGTGTGTTGTGTATGGATGT 122 26 0 34.62 58.200 6.00 0.00 7.800 230 ATGTAGCTTCTTGACTGTGTGTTGTG 144 26 0 42.31 61.844 4.00 0.00 7.844 231 GTCTGTTAAACATGTGTGGTATTGT 94 25 0 36.00 57.143 7.00 0.00 7.857 232 GTGTCTGTTAAACATGTGTGGTATT 92 25 0 36.00 57.143 7.00 1.00 7.857 233 TAAACATGTGTGGTATTGTGCATATAC 100 27 0 33.33 59.133 6.00 6.00 7.867 234 ATACATGTGTGTTGTGTATGGATGTAG 123 27 0 37.04 59.113 6.00 0.00 7.887 235 TATTGTGCATATACATGTGTGTTGT 113 25 0 32.00 57.073 6.00 2.00 7.927 236 GTATTGTGCATATACATGTGTGTTG 112 25 0 36.00 57.073 6.00 4.00 7.927 237 GCATATACATGTGTGTTGTGTATGGAT 119 27 0 37.04 61.010 6.00 2.00 8.010 238 GGTATTGTGCATATACATGTGTGTTGT 111 27 0 37.04 61.034 6.00 2.00 8.034 239 GTGGTATTGTGCATATACATGTGTGTT 109 27 0 37.04 61.034 6.00 4.00 8.034 240 TGTTGTGTATGGATGTAGCTTCTTGA 132 26 0 38.46 62.056 4.00 2.00 8.056 241 GTATTGTGCATATACATGTGTGTTGT 112 26 0 34.62 57.913 6.00 2.00 8.087 242 GTTGTGTATGGATGTAGCTTCTTGACT 133 27 0 40.74 61.126 4.00 1.00 8.126 243 GGTGTGTCTGTTAAACATGTGTGGTA 89 26 0 42.31 62.150 7.00 2.00 8.150 244 ACATGTGTGTTGTGTATGGATGTAGC 125 26 0 42.31 62.163 6.00 2.00 8.163 245 TCTGTTAAACATGTGTGGTATTGTGC 95 26 0 38.46 62.185 7.00 2.00 8.185 246 CATGTGTGTTGTGTATGGATGTAGCT 126 26 0 42.31 62.266 4.00 4.00 8.266 247 ATATACATGTGTGTTGTGTATGGATG 121 26 0 34.62 57.730 6.00 0.00 8.270 248 CATATACATGTGTGTTGTGTATGGAT 120 26 0 34.62 57.730 6.00 2.00 8.270 249 TGTGTTGTGTATAAATGTTGTGTCTT 49 26 0 30.77 57.700 4.00 0.00 8.300 250 TAGCTTCTTGACTGTGTGTTGTGTGT 147 26 0 42.31 62.367 4.00 0.00 8.367 251 GTAGCTTCTTGACTGTGTGTTGTGTG 146 26 0 46.15 62.367 4.00 0.00 8.367 252 TGTAGCTTCTTGACTGTGTGTTGTGT 145 26 0 42.31 62.367 4.00 0.00 8.367 253 TGTGTGTTGTGTATGGATGTAGCTTC 128 26 0 42.31 62.368 4.00 4.00 8.368 254 TGTTAAACATGTGTGGTATTGTGCAT 97 26 0 34.62 62.397 7.00 2.00 8.397 255 AAACATGTGTGGTATTGTGCATATACA 101 27 0 33.33 61.445 6.00 6.00 8.445 256 ATGGATGTAGCTTCTTGACTGTGTGT 140 26 0 42.31 62.454 4.00 0.00 8.454 257 ATATACATGTGTGTTGTGTATGGATGT 121 27 0 33.33 58.526 6.00 0.00 8.474 258 GTCTTGTGGGTGTGTCTGTTAAACAT 81 26 0 42.31 62.553 7.00 5.00 8.553 259 TGTGCATATACATGTGTGTTGTGTATG 116 27 0 37.04 61.688 6.00 5.00 8.688 260 TATACATGTGTGTTGTGTATGGATGTA 122 27 0 33.33 58.271 6.00 2.00 8.729 261 GTGTGTTGTGTATGGATGTAGCTTCTT 129 27 0 40.74 61.811 4.00 4.00 8.811 262 ATGTGTGGTATTGTGCATATACATGTG 105 27 0 37.04 61.814 6.00 4.00 8.814 263 CATGTGTGGTATTGTGCATATACATGT 104 27 0 37.04 61.814 6.00 6.00 8.814 264 ACATGTGTGGTATTGTGCATATACATG 103 27 0 37.04 61.814 6.00 6.00 8.814 265 ATGTGTTGTGTATAAATGTTGTGTCTT 48 27 0 29.63 58.041 4.00 0.00 8.959 266 TGTCTGTTAAACATGTGTGGTATTGTG 93 27 0 37.04 62.089 7.00 0.00 9.089 267 TGTGTCTGTTAAACATGTGTGGTATTG 91 27 0 37.04 62.089 7.00 0.00 9.089 268 TACATGTGTGTTGTGTATGGATGTAGC 124 27 0 40.74 62.100 6.00 2.00 9.100 269 GGTGTGTCTGTTAAACATGTGTGGTAT 89 27 0 40.74 62.286 7.00 2.00 9.286 270 GTGCATATACATGTGTGTTGTGTATGG 117 27 0 40.74 62.312 6.00 3.00 9.312 271 TGTGGTATTGTGCATATACATGTGTGT 108 27 0 37.04 62.312 6.00 6.00 9.312 272 GTGTGGTATTGTGCATATACATGTGTG 107 27 0 40.74 62.312 6.00 4.00 9.312 273 TGTGTGGTATTGTGCATATACATGTGT 106 27 0 37.04 62.312 6.00 6.00 9.312 274 TGTTAAACATGTGTGGTATTGTGCATA 97 27 0 33.33 62.329 7.00 4.00 9.329 275 TATGGATGTAGCTTCTTGACTGTGTGT 139 27 0 40.74 62.383 4.00 0.00 9.383 276 GTATGGATGTAGCTTCTTGACTGTGTG 138 27 0 44.44 62.383 4.00 0.00 9.383 277 TGTATGGATGTAGCTTCTTGACTGTGT 137 27 0 40.74 62.383 4.00 1.00 9.383 278 GTGTATGGATGTAGCTTCTTGACTGTG 136 27 0 44.44 62.383 4.00 1.00 9.383 279 TGTGTATGGATGTAGCTTCTTGACTGT 135 27 0 40.74 62.383 4.00 3.00 9.383 280 ATGTGTGTTGTGTATGGATGTAGCTTC 127 27 0 40.74 62.494 4.00 4.00 9.494 281 ATGTAGCTTCTTGACTGTGTGTTGTGT 144 27 0 40.74 62.495 4.00 0.00 9.495 282 TGTTGTGTATGGATGTAGCTTCTTGAC 132 27 0 40.74 62.685 4.00 1.00 9.685 283 GTGTTGTGTATGGATGTAGCTTCTTGA 131 27 0 40.74 62.685 4.00 2.00 9.685 284 TGGTATTGTGCATATACATGTGTGTTG 110 27 0 37.04 62.716 6.00 4.00 9.716 285 TTGTGTATGGATGTAGCTTCTTGACTG 134 27 0 40.74 62.778 4.00 1.00 9.778 286 GTCTGTTAAACATGTGTGGTATTGTGC 94 27 0 40.74 62.806 7.00 2.00 9.806 287 CATATGTGTTGTGTATAAATGTTGTGT 45 27 0 29.63 57.112 6.00 0.00 9.888 288 ACATATGTGTTGTGTATAAATGTTGTG 44 27 0 29.63 57.112 8.00 0.00 9.888 289 CACATATGTGTTGTGTATAAATGTTGT 43 27 0 29.63 57.112 10.00 2.00 9.888 290 ACACATATGTGTTGTGTATAAATGTTG 42 27 0 29.63 57.112 12.00 0.00 9.888 291 ACATGTGTGTTGTGTATGGATGTAGCT 125 27 0 40.74 62.902 6.00 4.00 9.902 primer3-2.3.6/test/primer_list_last/SP01-06-with-repeat2.int0000664000077300007730000003273312231156244022353 0ustar ioanaioanaACCEPTABLE INTERNAL OLIGOS 0-based # self self lib qual- # sequence start ln N GC% Tm any end sim lity 0 AAACGCAAAGCACGCTCC 113 18 0 55.56 62.465 4.00 0.00 8.00 4.465 1 AAAACGCAAAGCACGCTC 112 18 0 50.00 60.142 4.00 2.00 9.00 2.142 2 CAAAACGCAAAGCACGCTC 111 19 0 52.63 62.977 4.00 2.00 9.00 3.977 3 TCCAAAACGCAAAGCACG 109 18 0 50.00 61.944 2.00 2.00 9.00 3.944 4 TTCCAAAACGCAAAGCAC 108 18 0 44.44 58.389 2.00 0.00 9.00 3.611 5 CTTCCAAAACGCAAAGCAC 107 19 0 47.37 59.454 3.00 0.00 9.00 1.546 6 ACTTCCAAAACGCAAAGCAC 106 20 0 45.00 60.292 3.00 0.00 9.00 0.292 7 AACTTCCAAAACGCAAAGCAC 105 21 0 42.86 61.528 3.00 0.00 10.00 2.528 8 TAACTTCCAAAACGCAAAGCAC 104 22 0 40.91 61.484 3.00 0.00 10.00 3.484 9 CTAACTTCCAAAACGCAAAGCAC 103 23 0 43.48 62.256 3.00 0.00 10.00 5.256 10 CTTCCAAAACGCAAAGCA 107 18 0 44.44 58.546 3.00 0.00 9.00 3.454 11 ACTTCCAAAACGCAAAGCA 106 19 0 42.11 59.454 3.00 0.00 9.00 1.546 12 AACTTCCAAAACGCAAAGCA 105 20 0 40.00 60.782 3.00 0.00 10.00 0.782 13 TAACTTCCAAAACGCAAAGCA 104 21 0 38.10 60.764 3.00 0.00 10.00 1.764 14 CTAACTTCCAAAACGCAAAGCA 103 22 0 40.91 61.593 3.00 0.00 10.00 3.593 15 AACTTCCAAAACGCAAAGC 105 19 0 42.11 58.013 3.00 2.00 10.00 2.987 16 TAACTTCCAAAACGCAAAGC 104 20 0 40.00 58.097 3.00 2.00 10.00 1.903 17 CTAACTTCCAAAACGCAAAGC 103 21 0 42.86 59.065 3.00 2.00 10.00 1.935 18 TCTAACTTCCAAAACGCAAAGC 102 22 0 40.91 60.748 3.00 2.00 10.00 2.748 19 GTCTAACTTCCAAAACGCAAAGC 101 23 0 43.48 61.438 3.00 2.00 10.00 4.438 20 AGTCTAACTTCCAAAACGCAAAGC 100 24 0 41.67 62.180 4.00 2.00 10.00 6.180 21 TCTAACTTCCAAAACGCAAAG 102 21 0 38.10 57.272 3.00 0.00 10.00 3.728 22 GTCTAACTTCCAAAACGCAAAG 101 22 0 40.91 58.127 3.00 0.00 10.00 3.873 23 AGTCTAACTTCCAAAACGCAAAG 100 23 0 39.13 59.045 4.00 0.00 10.00 3.955 24 AAGTCTAACTTCCAAAACGCAAAG 99 24 0 37.50 60.206 6.00 0.00 10.00 4.206 25 AAAGTCTAACTTCCAAAACGCAAAG 98 25 0 36.00 61.265 6.00 0.00 10.00 6.265 26 GTCTAACTTCCAAAACGCAAA 101 21 0 38.10 57.124 3.00 0.00 10.00 3.876 27 AGTCTAACTTCCAAAACGCAAA 100 22 0 36.36 58.127 4.00 0.00 10.00 3.873 28 AAGTCTAACTTCCAAAACGCAAA 99 23 0 34.78 59.378 6.00 0.00 10.00 3.622 29 AAAGTCTAACTTCCAAAACGCAAA 98 24 0 33.33 60.517 6.00 0.00 10.00 4.517 30 CAAAGTCTAACTTCCAAAACGCAAA 97 25 0 36.00 62.766 6.00 0.00 10.00 7.766 31 AAGTCTAACTTCCAAAACGCAA 99 22 0 36.36 58.127 6.00 0.00 10.00 3.873 32 AAAGTCTAACTTCCAAAACGCAA 98 23 0 34.78 59.378 6.00 0.00 10.00 3.622 33 CAAAGTCTAACTTCCAAAACGCAA 97 24 0 37.50 61.761 6.00 0.00 10.00 5.761 34 AAAGTCTAACTTCCAAAACGCA 98 22 0 36.36 58.127 6.00 0.00 10.00 3.873 35 CAAAGTCTAACTTCCAAAACGCA 97 23 0 39.13 60.658 6.00 0.00 10.00 3.658 36 TCAAAGTCTAACTTCCAAAACGCA 96 24 0 37.50 62.242 6.00 0.00 10.00 6.242 37 GTCAAAGTCTAACTTCCAAAACGCA 95 25 0 40.00 62.860 6.00 0.00 10.00 7.860 38 CAAAGTCTAACTTCCAAAACGC 97 22 0 40.91 58.127 6.00 2.00 10.00 3.873 39 TCAAAGTCTAACTTCCAAAACGC 96 23 0 39.13 59.821 6.00 2.00 10.00 3.179 40 GTCAAAGTCTAACTTCCAAAACGC 95 24 0 41.67 60.537 6.00 2.00 10.00 4.537 41 TGTCAAAGTCTAACTTCCAAAACGC 94 25 0 40.00 62.860 6.00 2.00 10.00 7.860 42 GTCAAAGTCTAACTTCCAAAACG 95 23 0 39.13 57.204 6.00 2.00 10.00 5.796 43 TGTCAAAGTCTAACTTCCAAAACG 94 24 0 37.50 59.738 6.00 2.00 10.00 4.262 44 TTGTCAAAGTCTAACTTCCAAAACG 93 25 0 36.00 60.875 6.00 2.00 10.00 5.875 45 CTGTTGTCAAAGTCTAACTTCCAA 90 24 0 37.50 57.684 6.00 2.00 9.00 6.316 46 TCTGTTGTCAAAGTCTAACTTCCAA 89 25 0 36.00 59.394 6.00 2.00 9.00 5.606 47 GTCTGTTGTCAAAGTCTAACTTCCAA 88 26 0 38.46 60.127 6.00 2.00 9.00 6.127 48 AGTCTGTTGTCAAAGTCTAACTTCCAA 87 27 0 37.04 60.916 7.00 2.00 10.00 7.916 49 AGTCTGTTGTCAAAGTCTAACTTCC 87 25 0 40.00 57.318 7.00 2.00 10.00 7.682 50 GAGTCTGTTGTCAAAGTCTAACTTCC 86 26 0 42.31 58.988 7.00 2.00 10.00 7.012 51 TGAGTCTGTTGTCAAAGTCTAACTTCC 85 27 0 40.74 61.367 7.00 2.00 10.00 8.367 52 CAACTCACTGTATGAGTCTGTTGTC 73 25 0 44.00 57.993 6.00 1.00 11.00 7.007 53 CCAACTCACTGTATGAGTCTGTTGTC 72 26 0 46.15 61.357 6.00 2.00 11.00 7.357 54 AAACGCCAACTCACTGTATGA 67 21 0 42.86 58.296 3.00 2.00 10.00 2.704 55 GAAACGCCAACTCACTGTATGA 66 22 0 45.45 60.176 3.00 2.00 10.00 2.176 56 AGAAACGCCAACTCACTGTATGA 65 23 0 43.48 61.075 3.00 2.00 10.00 4.075 57 TAGAAACGCCAACTCACTGTATGA 64 24 0 41.67 61.047 3.00 2.00 10.00 5.047 58 ATAGAAACGCCAACTCACTGTATGA 63 25 0 40.00 61.238 3.00 2.00 10.00 6.238 59 GATAGAAACGCCAACTCACTGTATGA 62 26 0 42.31 62.768 3.00 2.00 10.00 8.768 60 GAAACGCCAACTCACTGTATG 66 21 0 47.62 58.296 3.00 1.00 10.00 2.704 61 AGAAACGCCAACTCACTGTATG 65 22 0 45.45 59.309 3.00 1.00 10.00 2.691 62 TAGAAACGCCAACTCACTGTATG 64 23 0 43.48 59.344 3.00 1.00 10.00 3.656 63 ATAGAAACGCCAACTCACTGTATG 63 24 0 41.67 59.623 3.00 1.00 10.00 4.377 64 GATAGAAACGCCAACTCACTGTATG 62 25 0 44.00 61.238 3.00 1.00 10.00 6.238 65 ATAGAAACGCCAACTCACTGTAT 63 23 0 39.13 57.058 3.00 2.00 10.00 5.942 66 GATAGAAACGCCAACTCACTGTAT 62 24 0 41.67 58.785 3.00 2.00 10.00 5.215 67 TGATAGAAACGCCAACTCACTGTAT 61 25 0 40.00 61.238 3.00 2.00 10.00 6.238 68 CTGATAGAAACGCCAACTCACTGTAT 60 26 0 42.31 61.990 3.00 2.00 10.00 7.990 69 GATAGAAACGCCAACTCACTGTA 62 23 0 43.48 58.464 3.00 2.00 10.00 4.536 70 TGATAGAAACGCCAACTCACTGTA 61 24 0 41.67 61.047 3.00 2.00 10.00 5.047 71 CTGATAGAAACGCCAACTCACTGTA 60 25 0 44.00 61.842 3.00 2.00 10.00 6.842 72 TGATAGAAACGCCAACTCACT 61 21 0 42.86 57.496 3.00 1.00 10.00 3.504 73 CTGATAGAAACGCCAACTCACT 60 22 0 45.45 58.536 3.00 1.00 10.00 3.464 74 CCTGATAGAAACGCCAACTCACT 59 23 0 47.83 61.863 3.00 1.00 10.00 4.863 75 ACCTGATAGAAACGCCAACTCACT 58 24 0 45.83 62.526 3.00 1.00 11.00 6.526 76 CTGATAGAAACGCCAACTCAC 60 21 0 47.62 57.496 3.00 0.00 10.00 3.504 77 CCTGATAGAAACGCCAACTCAC 59 22 0 50.00 61.033 3.00 0.00 10.00 3.033 78 ACCTGATAGAAACGCCAACTCAC 58 23 0 47.83 61.755 3.00 0.00 11.00 4.755 79 CCTGATAGAAACGCCAACTCA 59 21 0 47.62 60.256 3.00 1.00 10.00 1.256 80 ACCTGATAGAAACGCCAACTCA 58 22 0 45.45 61.033 3.00 1.00 11.00 3.033 81 GACCTGATAGAAACGCCAACTCA 57 23 0 47.83 62.713 3.00 1.00 11.00 5.713 82 CCTGATAGAAACGCCAACTC 59 20 0 50.00 57.422 2.00 0.00 10.00 2.578 83 ACCTGATAGAAACGCCAACTC 58 21 0 47.62 58.332 2.00 0.00 11.00 2.668 84 GACCTGATAGAAACGCCAACTC 57 22 0 50.00 60.137 2.00 0.00 11.00 2.137 85 TGACCTGATAGAAACGCCAACTC 56 23 0 47.83 62.713 2.00 0.00 11.00 5.713 86 ATGACCTGATAGAAACGCCAACTC 55 24 0 45.83 62.827 2.00 0.00 11.00 6.827 87 TATGACCTGATAGAAACGCCAACTC 54 25 0 44.00 62.743 4.00 0.00 11.00 7.743 88 GACCTGATAGAAACGCCAACT 57 21 0 47.62 58.332 2.00 1.00 11.00 2.668 89 TGACCTGATAGAAACGCCAACT 56 22 0 45.45 61.033 2.00 1.00 11.00 3.033 90 ATGACCTGATAGAAACGCCAACT 55 23 0 43.48 61.233 2.00 1.00 11.00 4.233 91 TATGACCTGATAGAAACGCCAACT 54 24 0 41.67 61.201 4.00 1.00 11.00 5.201 92 CTATGACCTGATAGAAACGCCAACT 53 25 0 44.00 61.959 6.00 1.00 11.00 6.959 93 GACCTGATAGAAACGCCAAC 57 20 0 50.00 57.265 2.00 0.00 11.00 2.735 94 TGACCTGATAGAAACGCCAAC 56 21 0 47.62 60.125 2.00 0.00 11.00 1.125 95 ATGACCTGATAGAAACGCCAAC 55 22 0 45.45 60.381 2.00 0.00 11.00 2.381 96 TATGACCTGATAGAAACGCCAAC 54 23 0 43.48 60.377 4.00 0.00 11.00 3.377 97 CTATGACCTGATAGAAACGCCAAC 53 24 0 45.83 61.201 6.00 0.00 11.00 5.201 98 TCTATGACCTGATAGAAACGCCAAC 52 25 0 44.00 62.743 8.00 0.00 11.00 7.743 99 TGACCTGATAGAAACGCCAA 56 20 0 45.00 59.272 2.00 0.00 11.00 0.728 100 ATGACCTGATAGAAACGCCAA 55 21 0 42.86 59.583 2.00 0.00 11.00 1.417 101 TATGACCTGATAGAAACGCCAA 54 22 0 40.91 59.609 4.00 0.00 11.00 2.391 102 CTATGACCTGATAGAAACGCCAA 53 23 0 43.48 60.495 6.00 0.00 11.00 3.495 103 TCTATGACCTGATAGAAACGCCAA 52 24 0 41.67 62.110 8.00 0.00 11.00 6.110 104 TGACCTGATAGAAACGCCA 56 19 0 47.37 57.759 2.00 0.00 11.00 3.241 105 ATGACCTGATAGAAACGCCA 55 20 0 45.00 58.173 2.00 0.00 11.00 1.827 106 TATGACCTGATAGAAACGCCA 54 21 0 42.86 58.254 4.00 0.00 11.00 2.746 107 CTATGACCTGATAGAAACGCCA 53 22 0 45.45 59.248 6.00 0.00 11.00 2.752 108 TCTATGACCTGATAGAAACGCCA 52 23 0 43.48 60.980 8.00 0.00 11.00 3.980 109 TTCTATGACCTGATAGAAACGCCA 51 24 0 41.67 62.110 10.00 0.00 11.00 6.110 110 TCTATGACCTGATAGAAACGCC 52 22 0 45.45 58.353 8.00 1.00 11.00 3.647 111 TTCTATGACCTGATAGAAACGCC 51 23 0 43.48 59.643 10.00 1.00 11.00 3.357 112 TTTCTATGACCTGATAGAAACGCC 50 24 0 41.67 60.811 12.00 1.00 11.00 4.811 113 TTTTCTATGACCTGATAGAAACGCC 49 25 0 40.00 61.874 12.00 2.00 11.00 6.874 114 TTTCTATGACCTGATAGAAACGC 50 23 0 39.13 57.615 12.00 2.00 11.00 5.385 115 CAATCAAATTTTTTCTATGACCTGAT 39 26 0 26.92 58.947 6.00 2.00 11.00 7.053 116 GCAATCAAATTTTTTCTATGACCTGAT 38 27 0 29.63 61.857 6.00 2.00 11.00 8.857 117 CAATCAAATTTTTTCTATGACCTGA 39 25 0 28.00 58.665 6.00 1.00 11.00 6.335 118 GCAATCAAATTTTTTCTATGACCTGA 38 26 0 30.77 61.716 6.00 1.00 11.00 7.716 119 CAATCAAATTTTTTCTATGACCTG 39 24 0 29.17 57.024 6.00 1.00 11.00 6.976 120 GCAATCAAATTTTTTCTATGACCTG 38 25 0 32.00 60.235 6.00 1.00 11.00 5.235 121 GCAATCAAATTTTTTCTATGACCT 38 24 0 29.17 57.882 6.00 1.00 11.00 6.118 122 CGCAATCAAATTTTTTCTATGACCT 37 25 0 32.00 61.717 6.00 1.00 11.00 6.717 123 CGCAATCAAATTTTTTCTATGACC 37 24 0 33.33 60.984 6.00 0.00 11.00 4.984 124 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 11.00 7.463 125 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 11.00 8.574 126 CGCAATCAAATTTTTTCTATGAC 37 23 0 30.43 57.905 6.00 1.00 10.00 5.095 127 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 10.00 4.458 128 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 10.00 5.211 129 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 10.00 6.843 130 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 10.00 9.235 131 CGCAATCAAATTTTTTCTATGA 37 22 0 27.27 57.077 6.00 2.00 10.00 4.923 132 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 10.00 4.207 133 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 10.00 5.202 134 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 10.00 7.653 135 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 10.00 4.923 136 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 10.00 5.544 137 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 10.00 5.323 138 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 10.00 5.202 139 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 11.00 8.397 140 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 12.00 9.507 141 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 10.00 5.439 142 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 11.00 4.023 143 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 12.00 5.202 144 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 12.00 6.204 145 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 12.00 8.199 146 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 10.00 4.533 147 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 11.00 3.033 148 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 12.00 4.199 149 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 12.00 5.202 150 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 12.00 7.226 151 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 12.00 8.382 152 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 11.00 2.909 153 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 12.00 3.626 154 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 12.00 4.596 155 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 12.00 5.502 156 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 12.00 6.694 157 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 12.00 8.652 primer3-2.3.6/test/primer_list_last/case_problem_test.for0000664000077300007730000000041012231156244022605 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 acctgggcaccacctactc 28 19 0 63.16 59.977 4.00 0.00 1.023 primer3-2.3.6/test/primer_list_last/SP04-07.txt.for0000664000077300007730000013025012231156244020651 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 CCCTACATACAGCCAAGGGA 21 20 0 55.00 59.948 4.00 0.00 0.052 1 TCCCTACATACAGCCAAGGG 20 20 0 55.00 59.948 4.00 1.00 0.052 2 CTAAGAATGTGGCCAAGGGA 122 20 0 50.00 60.066 6.00 0.00 0.066 3 TCCCTAAGAATGTGGCCAAG 119 20 0 50.00 60.066 6.00 2.00 0.066 4 GTTCCCTAAGAATGTGGCCA 117 20 0 50.00 59.933 6.00 6.00 0.067 5 TGTTCCCTAAGAATGTGGCC 116 20 0 50.00 59.933 4.00 4.00 0.067 6 GACTGCCACCGTGTACACAC 39 20 0 60.00 60.080 8.00 6.00 0.080 7 ACATACAGCCAAGGGACTGC 25 20 0 55.00 60.142 3.00 2.00 0.142 8 CCGCCATGTACACACTGAAG 95 20 0 55.00 60.175 6.00 2.00 0.175 9 TAAGAATGTGGCCAAGGGAA 123 20 0 45.00 60.439 6.00 0.00 0.439 10 TTCCCTAAGAATGTGGCCAA 118 20 0 45.00 60.439 6.00 4.00 0.439 11 GCCACCGTGTACACACTGAA 43 20 0 55.00 60.639 8.00 4.00 0.639 12 AAGAATGTGGCCAAGGGAAT 124 20 0 45.00 60.693 6.00 2.00 0.693 13 GAAATGTGGTTAAGGGACCG 75 20 0 50.00 59.288 4.00 2.00 0.712 14 TGGGTGTACCACACACTTCC 183 20 0 55.00 59.283 6.00 0.00 0.717 15 AATGCACTGAAGACCTTCCC 141 20 0 50.00 59.141 6.00 2.00 0.859 16 GGGAATGCACTGAAGACCTT 138 20 0 50.00 59.141 4.00 4.00 0.859 17 AAGGGAATGCACTGAAGACC 136 20 0 50.00 59.141 4.00 3.00 0.859 18 ACTGCCACCGTGTACACACT 40 20 0 55.00 59.101 8.00 6.00 0.899 19 GGACTGCCACCGTGTACAC 38 19 0 63.16 60.021 8.00 8.00 1.021 20 ATACAGCCAAGGGACTGCC 27 19 0 57.89 60.088 3.00 3.00 1.088 21 AAATGTGGTTAAGGGACCGC 76 20 0 50.00 61.099 4.00 2.00 1.099 22 TCCTACAGCTGTGGGAAAATG 200 21 0 47.62 60.118 12.00 0.00 1.118 23 AATGCACTGAAGACCTTCCCT 141 21 0 47.62 60.125 6.00 4.00 1.125 24 AGGGAATGCACTGAAGACCTT 137 21 0 47.62 60.125 4.00 4.00 1.125 25 AAGGGAATGCACTGAAGACCT 136 21 0 47.62 60.125 4.00 2.00 1.125 26 TGCACTGAAGACCTTCCCTAA 143 21 0 47.62 59.861 6.00 1.00 1.139 27 TACATACAGCCAAGGGACTGC 24 21 0 52.38 60.147 3.00 2.00 1.147 28 CCAAGGGAATGCACTGAAGA 134 20 0 50.00 61.176 4.00 0.00 1.176 29 GTGGGTGTACCACACACTTCC 182 21 0 57.14 60.189 8.00 0.00 1.189 30 AATGTGGTTAAGGGACCGC 77 19 0 52.63 59.805 4.00 2.00 1.195 31 ATGTTCCCTAAGAATGTGGCC 115 21 0 47.62 60.201 4.00 4.00 1.201 32 TGCCACCGTGTACACACTG 42 19 0 57.89 60.217 8.00 8.00 1.217 33 AGAAATGTGGTTAAGGGACCG 74 21 0 47.62 60.228 4.00 2.00 1.228 34 ATGCACTGAAGACCTTCCCT 142 20 0 50.00 58.744 6.00 4.00 1.256 35 AGGGAATGCACTGAAGACCT 137 20 0 50.00 58.744 4.00 2.00 1.256 36 CTGCCACCGTGTACACACTG 41 20 0 60.00 61.274 8.00 8.00 1.274 37 CATTGTGGGTGTACCACACA 178 20 0 50.00 58.674 8.00 8.00 1.326 38 TGGGTGTACCACACACTTCCT 183 21 0 52.38 60.328 6.00 0.00 1.328 39 CATTGTGGGTGTACCACACAC 178 21 0 52.38 59.632 8.00 8.00 1.368 40 ACATTGTGGGTGTACCACACA 177 21 0 47.62 59.632 8.00 8.00 1.368 41 CCGTGTACACACTGAACACGT 47 21 0 52.38 59.573 9.00 7.00 1.427 42 AAGAATGTGGCCAAGGGAA 124 19 0 47.37 60.445 6.00 0.00 1.445 43 CACGTTCCCTAAGAAATGTGG 63 21 0 47.62 59.480 8.00 6.00 1.520 44 CCACCGTGTACACACTGAACA 44 21 0 52.38 60.530 8.00 5.00 1.530 45 TGCACTGAAGACCTTCCCTA 143 20 0 50.00 58.442 6.00 2.00 1.558 46 AGAATGTGGCCAAGGGAAT 125 19 0 47.37 59.351 6.00 2.00 1.649 47 TGTGGGTGTACCACACACTTC 181 21 0 52.38 59.347 8.00 2.00 1.653 48 TAAGAATGTGGCCAAGGGAAT 123 21 0 42.86 60.678 6.00 2.00 1.678 49 CAAGGGAATGCACTGAAGAC 135 20 0 50.00 58.287 4.00 1.00 1.713 50 CGCCATGTACACACTGAAGA 96 20 0 50.00 58.276 6.00 1.00 1.724 51 CCTAAGAATGTGGCCAAGGG 121 20 0 55.00 61.732 6.00 1.00 1.732 52 CCCTAAGAATGTGGCCAAGG 120 20 0 55.00 61.732 6.00 2.00 1.732 53 CATACAGCCAAGGGACTGC 26 19 0 57.89 59.256 3.00 2.00 1.744 54 CCCTACATACAGCCAAGGGAC 21 21 0 57.14 60.748 4.00 1.00 1.748 55 CCAAGGGAATGCACTGAAG 134 19 0 52.63 59.226 4.00 2.00 1.774 56 GCCACCGTGTACACACTGA 43 19 0 57.89 59.114 8.00 6.00 1.886 57 GACTGCCACCGTGTACACA 39 19 0 57.89 59.114 8.00 6.00 1.886 58 CCGCCATGTACACACTGAA 95 19 0 52.63 59.097 6.00 1.00 1.903 59 TAAGAATGTGGCCAAGGGA 123 19 0 47.37 59.061 6.00 0.00 1.939 60 TCCCTAAGAATGTGGCCAA 119 19 0 47.37 59.061 6.00 4.00 1.939 61 TTCCCTAAGAATGTGGCCA 118 19 0 47.37 59.061 6.00 6.00 1.939 62 ACACACTGAACACGTTCCCT 53 20 0 50.00 58.039 6.00 4.00 1.961 63 AGGGACTGCCACCGTGTAC 36 19 0 63.16 61.004 5.00 4.00 2.004 64 GAATGCACTGAAGACCTTCCC 140 21 0 52.38 61.014 6.00 2.00 2.014 65 GGAATGCACTGAAGACCTTCC 139 21 0 52.38 61.014 6.00 4.00 2.014 66 GGGAATGCACTGAAGACCTTC 138 21 0 52.38 61.014 6.00 6.00 2.014 67 GGGACTGCCACCGTGTAC 37 18 0 66.67 59.962 5.00 4.00 2.038 68 GACTGCCACCGTGTACACACT 39 21 0 57.14 61.098 8.00 6.00 2.098 69 AGGGACTGCCACCGTGTA 36 18 0 61.11 60.113 5.00 2.00 2.113 70 TGCACTGAAGACCTTCCCTAAT 143 22 0 45.45 60.130 6.00 2.00 2.130 71 ATGCACTGAAGACCTTCCCTAA 142 22 0 45.45 60.130 6.00 1.00 2.130 72 AATGCACTGAAGACCTTCCCTA 141 22 0 45.45 60.130 6.00 2.00 2.130 73 AGAATGTGGCCAAGGGAATG 125 20 0 50.00 62.168 6.00 0.00 2.168 74 TTGTGGGTGTACCACACACTT 180 21 0 47.62 58.817 8.00 5.00 2.183 75 AAGACATTGTGGGTGTACCACA 174 22 0 45.45 60.193 6.00 6.00 2.193 76 ATGCACTGAAGACCTTCCCTA 142 21 0 47.62 58.803 6.00 2.00 2.197 77 TACAGCCAAGGGACTGCC 28 18 0 61.11 59.787 3.00 3.00 2.213 78 GTTCCCTAAGAATGTGGCCAA 117 21 0 47.62 61.214 6.00 4.00 2.214 79 TTCCCTACATACAGCCAAGGG 19 21 0 52.38 61.221 4.00 1.00 2.221 80 AGACATTGTGGGTGTACCACA 175 21 0 47.62 58.765 6.00 6.00 2.235 81 GAATGTGGCCAAGGGAATG 126 19 0 52.63 61.265 6.00 0.00 2.265 82 CTTCCCTACATACAGCCAAGG 18 21 0 52.38 58.730 4.00 2.00 2.270 83 CACGTTCCCTAAGAAATGTGGT 63 22 0 45.45 60.275 8.00 1.00 2.275 84 ACACGTTCCCTAAGAAATGTGG 62 22 0 45.45 60.275 8.00 6.00 2.275 85 TTCCCTACATACAGCCAAGG 19 20 0 50.00 57.700 3.00 2.00 2.300 86 CACACTGAACACGTTCCCTAAG 54 22 0 50.00 59.696 6.00 3.00 2.304 87 TGCCACCGTGTACACACTGA 42 20 0 55.00 62.305 8.00 6.00 2.305 88 CACACTGAACACGTTCCCTAA 54 21 0 47.62 58.687 6.00 1.00 2.313 89 AAGGGACTGCCACCGTGTAC 35 20 0 60.00 62.314 5.00 4.00 2.314 90 GCCGCCATGTACACACTG 94 18 0 61.11 59.685 6.00 4.00 2.315 91 TGGGTGTACCACACACTTCCTA 183 22 0 50.00 60.326 6.00 2.00 2.326 92 AGACATTGTGGGTGTACCACAC 175 22 0 50.00 59.670 6.00 6.00 2.330 93 CTAAGAATGTGGCCAAGGGAA 122 21 0 47.62 61.331 6.00 0.00 2.331 94 TTCCCTAAGAATGTGGCCAAG 118 21 0 47.62 61.331 6.00 2.00 2.331 95 CGCCATGTACACACTGAAGAT 96 21 0 47.62 58.664 6.00 3.00 2.336 96 GACATTGTGGGTGTACCACAC 176 21 0 52.38 58.609 6.00 6.00 2.391 97 GACATTGTGGGTGTACCACA 176 20 0 50.00 57.608 6.00 6.00 2.392 98 ACACACTGAACACGTTCCCTAA 53 22 0 45.45 59.562 6.00 1.00 2.438 99 ACCGTGTACACACTGAACACGT 46 22 0 50.00 60.445 9.00 7.00 2.445 100 ACTTCCCTACATACAGCCAAGG 17 22 0 50.00 59.543 4.00 2.00 2.457 101 GATGTTCCCTAAGAATGTGGC 114 21 0 47.62 58.517 3.00 2.00 2.483 102 GCCACCGTGTACACACTGAAC 43 21 0 57.14 61.486 8.00 4.00 2.486 103 ACATTGTGGGTGTACCACACAC 177 22 0 50.00 60.510 8.00 8.00 2.510 104 AGATGTTCCCTAAGAATGTGGC 113 22 0 45.45 59.487 3.00 2.00 2.513 105 AAGGGACTGCCACCGTGTA 35 19 0 57.89 61.530 5.00 2.00 2.530 106 CCACCGTGTACACACTGAAC 44 20 0 55.00 57.400 8.00 3.00 2.600 107 TGCACTGAAGACCTTCCCT 143 19 0 52.63 58.364 6.00 4.00 2.636 108 CATTGTGGGTGTACCACACACT 178 22 0 50.00 60.644 8.00 8.00 2.644 109 TCACACTCACTGAAGACTTCCC 2 22 0 50.00 59.350 7.00 3.00 2.650 110 GGGTGTACCACACACTTCCT 184 20 0 55.00 57.315 5.00 0.00 2.685 111 AATGTGGCCAAGGGAATG 127 18 0 50.00 59.296 6.00 0.00 2.704 112 GAATGCACTGAAGACCTTCC 140 20 0 50.00 57.288 6.00 4.00 2.712 113 GGAATGCACTGAAGACCTTC 139 20 0 50.00 57.288 6.00 6.00 2.712 114 CACTGAACACGTTCCCTAAGAA 56 22 0 45.45 59.281 6.00 2.00 2.719 115 TCCTACAGCTGTGGGAAAAT 200 20 0 45.00 57.268 12.00 2.00 2.732 116 TCCCTACATACAGCCAAGGGA 20 21 0 52.38 61.748 5.00 0.00 2.748 117 TTGTGGGTGTACCACACACTTC 180 22 0 50.00 60.765 8.00 3.00 2.765 118 TGTGGGTGTACCACACACTT 181 20 0 50.00 57.229 8.00 5.00 2.771 119 TTGTGGGTGTACCACACACT 180 20 0 50.00 57.229 8.00 6.00 2.771 120 GCCGCCATGTACACACTGA 94 19 0 57.89 61.775 6.00 2.00 2.775 121 GCCAAGGGAATGCACTGA 133 18 0 55.56 60.784 4.00 1.00 2.784 122 CCTACATACAGCCAAGGGACT 22 21 0 52.38 58.214 3.00 1.00 2.786 123 CACACACTTCCTACAGCTGTGG 192 22 0 54.55 60.796 8.00 8.00 2.796 124 CCACACACTTCCTACAGCTGTG 191 22 0 54.55 60.796 8.00 6.00 2.796 125 CGTGTACACACTGAACACGTTC 48 22 0 50.00 59.186 9.00 6.00 2.814 126 ATTGTGGGTGTACCACACACTT 179 22 0 45.45 59.161 8.00 6.00 2.839 127 CTAAGAATGTGGCCAAGGG 122 19 0 52.63 58.159 6.00 1.00 2.841 128 CCTAAGAATGTGGCCAAGG 121 19 0 52.63 58.159 6.00 2.00 2.841 129 CCCTAAGAATGTGGCCAAG 120 19 0 52.63 58.159 6.00 2.00 2.841 130 CCTACATACAGCCAAGGGAC 22 20 0 55.00 57.143 3.00 1.00 2.857 131 CACACTGAACACGTTCCCTA 54 20 0 50.00 57.141 6.00 2.00 2.859 132 TGAACACGTTCCCTAAGAAATG 59 22 0 40.91 59.131 6.00 4.00 2.869 133 ACACACTGAACACGTTCCCTA 53 21 0 47.62 58.130 6.00 2.00 2.870 134 TACACACTGAACACGTTCCCT 52 21 0 47.62 58.130 6.00 4.00 2.870 135 CGTTCCCTAAGAAATGTGGTT 65 21 0 42.86 58.111 4.00 0.00 2.889 136 CTGCCACCGTGTACACACT 41 19 0 57.89 58.109 8.00 6.00 2.891 137 TGTACACACTGAACACGTTCCC 50 22 0 50.00 60.906 6.00 2.00 2.906 138 ATACAGCCAAGGGACTGCCA 27 20 0 55.00 62.908 3.00 3.00 2.908 139 CATACAGCCAAGGGACTGCC 26 20 0 60.00 62.908 3.00 3.00 2.908 140 CCTACATACAGCCAAGGGACTG 22 22 0 54.55 60.913 3.00 3.00 2.913 141 CCGTGTACACACTGAACACGTT 47 22 0 50.00 60.956 9.00 5.00 2.956 142 GGACTGCCACCGTGTACA 38 18 0 61.11 59.043 6.00 6.00 2.957 143 CAAGGGAATGCACTGAAGACC 135 21 0 52.38 61.957 4.00 3.00 2.957 144 CCAAGGGAATGCACTGAAGAC 134 21 0 52.38 61.957 4.00 1.00 2.957 145 GTACACACTGAACACGTTCCCT 51 22 0 50.00 59.041 6.00 4.00 2.959 146 CCCTACATACAGCCAAGGG 21 19 0 57.89 58.034 4.00 1.00 2.966 147 AGAATGTGGCCAAGGGAA 125 18 0 50.00 59.009 6.00 0.00 2.991 148 AAGAATGTGGCCAAGGGA 124 18 0 50.00 59.009 6.00 0.00 2.991 149 GTACACACTGAACACGTTCCC 51 21 0 52.38 57.973 6.00 2.00 3.027 150 CTGAACACGTTCCCTAAGAAATG 58 23 0 43.48 60.042 6.00 4.00 3.042 151 CTACATACAGCCAAGGGACTGC 23 22 0 54.55 61.052 3.00 2.00 3.052 152 ATGTGGTTAAGGGACCGCC 78 19 0 57.89 62.052 4.00 1.00 3.052 153 ACTGCCACCGTGTACACAC 40 19 0 57.89 57.937 8.00 6.00 3.063 154 ACGTTCCCTAAGAAATGTGGTT 64 22 0 40.91 58.937 6.00 0.00 3.063 155 CCCTAAGAAATGTGGTTAAGGG 69 22 0 45.45 58.926 4.00 4.00 3.074 156 GAACACGTTCCCTAAGAAATGTG 60 23 0 43.48 59.920 8.00 8.00 3.080 157 TGAACACGTTCCCTAAGAAATGT 59 23 0 39.13 59.920 6.00 6.00 3.080 158 ACACTGAACACGTTCCCTAAGAA 55 23 0 43.48 60.082 6.00 2.00 3.082 159 CCGCCATGTACACACTGAAGA 95 21 0 52.38 62.092 6.00 1.00 3.092 160 ACTGCCACCGTGTACACACTG 40 21 0 57.14 62.102 8.00 8.00 3.102 161 ACACACTTCCTACAGCTGTGG 193 21 0 52.38 57.875 8.00 8.00 3.125 162 CCACACACTTCCTACAGCTGT 191 21 0 52.38 57.875 8.00 8.00 3.125 163 ACCACACACTTCCTACAGCTG 190 21 0 52.38 57.875 6.00 6.00 3.125 164 CACTGAACACGTTCCCTAAGA 56 21 0 47.62 57.871 6.00 1.00 3.129 165 TGCACTGAAGACCTTCCCTAATA 143 23 0 43.48 60.136 6.00 3.00 3.136 166 GTGGGTGTACCACACACTTCCT 182 22 0 54.55 61.149 8.00 0.00 3.149 167 GCCAAGGGAATGCACTGAA 133 19 0 52.63 62.155 4.00 3.00 3.155 168 TAAGACATTGTGGGTGTACCACA 173 23 0 43.48 60.196 6.00 6.00 3.196 169 ACCACACACTTCCTACAGCTGT 190 22 0 50.00 58.799 8.00 8.00 3.201 170 ACACTGAACACGTTCCCTAAGA 55 22 0 45.45 58.769 6.00 1.00 3.231 171 ATTGTGGGTGTACCACACACT 179 21 0 47.62 57.686 8.00 8.00 3.314 172 GAAGACTTCCCTACATACAGCCA 13 23 0 47.83 59.666 7.00 0.00 3.334 173 TGAAGACTTCCCTACATACAGCC 12 23 0 47.83 59.666 7.00 1.00 3.334 174 TCACACTCACTGAAGACTTCCCT 2 23 0 47.83 60.337 7.00 5.00 3.337 175 ATCACACTCACTGAAGACTTCCC 1 23 0 47.83 59.659 7.00 3.00 3.341 176 AAGGGAATGCACTGAAGACCTT 136 22 0 45.45 61.364 4.00 4.00 3.364 177 ATGCACTGAAGACCTTCCC 142 19 0 52.63 57.630 6.00 2.00 3.370 178 GGGAATGCACTGAAGACCT 138 19 0 52.63 57.630 4.00 2.00 3.370 179 AGGGAATGCACTGAAGACC 137 19 0 52.63 57.630 4.00 3.00 3.370 180 ATGCACTGAAGACCTTCCCTAAT 142 23 0 43.48 60.373 6.00 2.00 3.373 181 CCACCGTGTACACACTGAACAC 44 22 0 54.55 61.374 8.00 7.00 3.374 182 ACGTTCCCTAAGAAATGTGGT 64 21 0 42.86 57.593 6.00 0.00 3.407 183 TACACACTGAACACGTTCCCTAA 52 23 0 43.48 59.588 6.00 1.00 3.412 184 AAGAAATGTGGTTAAGGGACCG 73 22 0 45.45 61.417 4.00 2.00 3.417 185 GCTAAGACATTGTGGGTGTACC 171 22 0 50.00 58.513 5.00 4.00 3.487 186 ACACACTGAACACGTTCCCTAAG 53 23 0 47.83 60.494 6.00 3.00 3.494 187 CTAAGAATGTGGCCAAGGGAAT 122 22 0 45.45 61.515 6.00 2.00 3.515 188 CACCGTGTACACACTGAACAC 45 21 0 52.38 57.482 8.00 7.00 3.518 189 AGCTAAGACATTGTGGGTGTACC 170 23 0 47.83 59.468 5.00 4.00 3.532 190 CGCCATGTACACACTGAAGATG 96 22 0 50.00 61.537 6.00 5.00 3.537 191 CACTGAACACGTTCCCTAAGAAA 56 23 0 43.48 60.550 6.00 4.00 3.550 192 CACACTCACTGAAGACTTCCCT 3 22 0 50.00 58.449 7.00 5.00 3.551 193 CCCTAAGAAATGTGGTTAAGGGA 69 23 0 43.48 60.553 4.00 0.00 3.553 194 TCCCTAAGAAATGTGGTTAAGGG 68 23 0 43.48 60.553 4.00 4.00 3.553 195 CAAAAGCTAAGACATTGTGGG 166 21 0 42.86 57.442 5.00 0.00 3.558 196 CGTTCCCTAAGAAATGTGGTTAA 65 23 0 39.13 59.441 4.00 4.00 3.559 197 GGGTGTACCACACACTTCCTA 184 21 0 52.38 57.434 5.00 2.00 3.566 198 AAGGGACTGCCACCGTGT 35 18 0 61.11 61.583 5.00 3.00 3.583 199 GCACTGAAGACCTTCCCTAAT 144 21 0 47.62 57.417 6.00 2.00 3.583 200 GACATTGTGGGTGTACCACACA 176 22 0 50.00 61.591 8.00 8.00 3.591 201 CCCTACATACAGCCAAGGGACT 21 22 0 54.55 61.601 4.00 1.00 3.601 202 AAATGTGGTTAAGGGACCG 76 19 0 47.37 57.380 4.00 2.00 3.620 203 CTACATACAGCCAAGGGACTG 23 21 0 52.38 57.376 3.00 3.00 3.624 204 GGGTGTACCACACACTTCCTAC 184 22 0 54.55 58.361 5.00 0.00 3.639 205 ATGTGGTTAAGGGACCGC 78 18 0 55.56 58.359 4.00 2.00 3.641 206 CACACTCACTGAAGACTTCCC 3 21 0 52.38 57.332 7.00 3.00 3.668 207 TGTTCCCTAAGAATGTGGCCA 116 21 0 47.62 62.668 6.00 6.00 3.668 208 AAAAGCTAAGACATTGTGGGTG 167 22 0 40.91 58.314 5.00 0.00 3.686 209 CAAAAGCTAAGACATTGTGGGT 166 22 0 40.91 58.314 5.00 0.00 3.686 210 ACAAAAGCTAAGACATTGTGGG 165 22 0 40.91 58.314 5.00 1.00 3.686 211 AAGATGTTCCCTAAGAATGTGGC 112 23 0 43.48 60.707 4.00 2.00 3.707 212 CAAGGGAATGCACTGAAGA 135 19 0 47.37 57.292 4.00 0.00 3.708 213 GAATGTGGCCAAGGGAAT 126 18 0 50.00 58.274 6.00 2.00 3.726 214 AAGACATTGTGGGTGTACCAC 174 21 0 47.62 57.273 5.00 4.00 3.727 215 CGCCGCCATGTACACACT 93 18 0 61.11 61.777 6.00 2.00 3.777 216 TACAGCCAAGGGACTGCCA 28 19 0 57.89 62.781 3.00 3.00 3.781 217 TACACACTGAACACGTTCCCTA 52 22 0 45.45 58.214 6.00 2.00 3.786 218 AAGACTTCCCTACATACAGCCAA 14 23 0 43.48 59.209 5.00 2.00 3.791 219 GAAATGTGGTTAAGGGACCGC 75 21 0 52.38 62.792 4.00 2.00 3.792 220 CGTTCCCTAAGAAATGTGGTTA 65 22 0 40.91 58.188 4.00 2.00 3.812 221 AACACGTTCCCTAAGAAATGTG 61 22 0 40.91 58.175 8.00 8.00 3.825 222 CGTGTACACACTGAACACGTT 48 21 0 47.62 57.155 9.00 4.00 3.845 223 ATGTGGCCAAGGGAATGC 128 18 0 55.56 61.869 6.00 2.00 3.869 224 TGTGGTTAAGGGACCGCC 79 18 0 61.11 61.876 4.00 1.00 3.876 225 AAAAGCTAAGACATTGTGGGTGT 167 23 0 39.13 59.123 5.00 0.00 3.877 226 ACAAAAGCTAAGACATTGTGGGT 165 23 0 39.13 59.123 5.00 1.00 3.877 227 GAATGCACTGAAGACCTTCCCT 140 22 0 50.00 61.884 6.00 4.00 3.884 228 AGGGAATGCACTGAAGACCTTC 137 22 0 50.00 61.884 6.00 6.00 3.884 229 TCCTACAGCTGTGGGAAAATGA 200 22 0 45.45 61.895 12.00 1.00 3.895 230 CCAAGGGAATGCACTGAA 134 18 0 50.00 58.095 4.00 3.00 3.905 231 CAAAAGCTAAGACATTGTGGGTG 166 23 0 43.48 60.909 5.00 0.00 3.909 232 GTACACACTGAACACGTTCCCTA 51 23 0 47.83 59.087 6.00 2.00 3.913 233 GATGTTCCCTAAGAATGTGGCC 114 22 0 50.00 61.913 4.00 4.00 3.913 234 AAGAAATGTGGTTAAGGGACC 73 21 0 42.86 57.076 4.00 3.00 3.924 235 TGTACACACTGAACACGTTCC 50 21 0 47.62 57.053 6.00 4.00 3.947 236 GTGTACACACTGAACACGTTCC 49 22 0 50.00 58.046 8.00 4.00 3.954 237 GCCATGTACACACTGAAGATGTT 97 23 0 43.48 59.046 6.00 4.00 3.954 238 GTTCCCTAAGAATGTGGCC 117 19 0 52.63 57.030 4.00 4.00 3.970 239 CTGAACACGTTCCCTAAGAAA 58 21 0 42.86 57.025 6.00 4.00 3.975 240 GAAGATGTTCCCTAAGAATGTGG 111 23 0 43.48 59.014 6.00 0.00 3.986 241 ATTGTGGGTGTACCACACACTTC 179 23 0 47.83 60.995 8.00 5.00 3.995 242 AAGACATTGTGGGTGTACCACAC 174 23 0 47.83 60.995 6.00 6.00 3.995 243 CACACTGAACACGTTCCCT 54 19 0 52.63 57.003 6.00 4.00 3.997 244 ACACGTTCCCTAAGAAATGTGGT 62 23 0 43.48 61.013 8.00 6.00 4.013 245 ACGTTCCCTAAGAAATGTGGTTA 64 23 0 39.13 58.983 6.00 2.00 4.017 246 TACCACACACTTCCTACAGCTG 189 22 0 50.00 57.969 6.00 6.00 4.031 247 GTTCCCTAAGAATGTGGCCAAG 117 22 0 50.00 62.039 6.00 2.00 4.039 248 CTTCCCTACATACAGCCAAGGG 18 22 0 54.55 62.041 4.00 1.00 4.041 249 AAGACTTCCCTACATACAGCCAAG 14 24 0 45.83 60.076 5.00 4.00 4.076 250 ACTGAACACGTTCCCTAAGAAA 57 22 0 40.91 57.921 6.00 4.00 4.079 251 GACTTCCCTACATACAGCCAAG 16 22 0 50.00 57.893 4.00 4.00 4.107 252 AGACTTCCCTACATACAGCCAA 15 22 0 45.45 57.893 3.00 2.00 4.107 253 AAGACTTCCCTACATACAGCCA 14 22 0 45.45 57.893 5.00 0.00 4.107 254 GGGTGTACCACACACTTCCTACA 184 23 0 52.17 61.117 5.00 1.00 4.117 255 TGGGTGTACCACACACTTCCTAC 183 23 0 52.17 61.117 6.00 0.00 4.117 256 GTGGGTGTACCACACACTTCCTA 182 23 0 52.17 61.117 8.00 2.00 4.117 257 AGACTTCCCTACATACAGCCAAG 15 23 0 47.83 58.861 4.00 4.00 4.139 258 TACCACACACTTCCTACAGCTGT 189 23 0 47.83 58.855 8.00 8.00 4.145 259 GTACCACACACTTCCTACAGCTG 188 23 0 52.17 58.855 6.00 6.00 4.145 260 TGTACCACACACTTCCTACAGCT 187 23 0 47.83 58.855 4.00 4.00 4.145 261 ACGTTCCCTAAGAAATGTGGTTAA 64 24 0 37.50 60.157 6.00 4.00 4.157 262 TGTACCACACACTTCCTACAGC 187 22 0 50.00 57.817 4.00 2.00 4.183 263 GCTAAGACATTGTGGGTGTACCA 171 23 0 47.83 61.184 5.00 2.00 4.184 264 AAAGCTAAGACATTGTGGGTGT 168 22 0 40.91 57.804 5.00 0.00 4.196 265 GACTTCCCTACATACAGCCAAGG 16 23 0 52.17 61.222 4.00 2.00 4.222 266 CCGCCATGTACACACTGAAGAT 95 22 0 50.00 62.253 6.00 3.00 4.253 267 CGTTCCCTAAGAAATGTGGTTAAG 65 24 0 41.67 60.268 4.00 2.00 4.268 268 GTGTACCACACACTTCCTACAGC 186 23 0 52.17 58.715 5.00 2.00 4.285 269 CACACTGAAGATGTTCCCTAAGAA 105 24 0 41.67 59.690 6.00 2.00 4.310 270 GTACCACACACTTCCTACAGCTGT 188 24 0 50.00 59.674 8.00 8.00 4.326 271 GTGTACCACACACTTCCTACAGCT 186 24 0 50.00 59.674 5.00 4.00 4.326 272 AATGCACTGAAGACCTTCCCTAA 141 23 0 43.48 61.327 6.00 1.00 4.327 273 TCACACTCACTGAAGACTTCCCTA 2 24 0 45.83 60.335 7.00 3.00 4.335 274 TGTACACACTGAAGATGTTCCCT 101 23 0 43.48 58.639 6.00 4.00 4.361 275 GTACACACTGAACACGTTCCCTAA 51 24 0 45.83 60.367 6.00 1.00 4.367 276 ATGCACTGAAGACCTTCCCTAATA 142 24 0 41.67 60.370 6.00 3.00 4.370 277 TAAGAAATGTGGTTAAGGGACCG 72 23 0 43.48 61.379 4.00 2.00 4.379 278 TTCCCTAAGAAATGTGGTTAAGG 67 23 0 39.13 58.619 4.00 4.00 4.381 279 GCCATGTACACACTGAAGATGT 97 22 0 45.45 57.601 6.00 4.00 4.399 280 TGTACACACTGAAGATGTTCCC 101 22 0 45.45 57.574 6.00 2.00 4.426 281 GGTGTACCACACACTTCCTACAG 185 23 0 52.17 58.570 5.00 2.00 4.430 282 ACATTGTGGGTGTACCACACACT 177 23 0 47.83 61.441 8.00 8.00 4.441 283 CACGTTCCCTAAGAAATGTGGTT 63 23 0 43.48 61.453 8.00 0.00 4.453 284 AACACGTTCCCTAAGAAATGTGG 61 23 0 43.48 61.453 8.00 6.00 4.453 285 CTAAGACATTGTGGGTGTACCA 172 22 0 45.45 57.543 5.00 2.00 4.457 286 TCCCTACATACAGCCAAGGGAC 20 22 0 54.55 62.458 5.00 1.00 4.458 287 CACACTGAACACGTTCCCTAAGA 54 23 0 47.83 61.476 6.00 1.00 4.476 288 GCACTGAAGACCTTCCCTAATA 144 22 0 45.45 57.520 6.00 3.00 4.480 289 CACTCACTGAAGACTTCCCTACA 5 23 0 47.83 58.519 7.00 1.00 4.481 290 CACACTCACTGAAGACTTCCCTA 3 23 0 47.83 58.519 7.00 3.00 4.481 291 TCCCTAAGAATGTGGCCA 119 18 0 50.00 57.514 6.00 6.00 4.486 292 TACACACTGAACACGTTCCCTAAG 52 24 0 45.83 60.486 6.00 3.00 4.486 293 GGTGTACCACACACTTCCTACA 185 22 0 50.00 57.509 5.00 1.00 4.491 294 GTGGCCAAGGGAATGCAC 130 18 0 61.11 62.504 6.00 2.00 4.504 295 CTGAAGACTTCCCTACATACAGCC 11 24 0 50.00 60.533 7.00 1.00 4.533 296 CTGAACACGTTCCCTAAGAAAT 58 22 0 40.91 57.436 6.00 2.00 4.564 297 CTAAGACATTGTGGGTGTACCAC 172 23 0 47.83 58.433 5.00 4.00 4.567 298 CCGCCATGTACACACTGA 95 18 0 55.56 57.425 6.00 2.00 4.575 299 ACCACACACTTCCTACAGCTGTG 190 23 0 52.17 61.580 8.00 6.00 4.580 300 ATCACACTCACTGAAGACTTCCCT 1 24 0 45.83 60.582 7.00 5.00 4.582 301 TAAGACATTGTGGGTGTACCAC 173 22 0 45.45 57.389 5.00 4.00 4.611 302 TACAAAAGCTAAGACATTGTGGG 164 23 0 39.13 58.382 5.00 1.00 4.618 303 CACACTGAAGATGTTCCCTAAGA 105 23 0 43.48 58.382 6.00 1.00 4.618 304 GGCCAAGGGAATGCACTG 132 18 0 61.11 62.628 4.00 3.00 4.628 305 TGGCCAAGGGAATGCACT 131 18 0 55.56 62.628 6.00 4.00 4.628 306 ACACTCACTGAAGACTTCCCTACA 4 24 0 45.83 59.361 7.00 1.00 4.639 307 CACACTCACTGAAGACTTCCCTAC 3 24 0 50.00 59.361 7.00 1.00 4.639 308 GTTCCCTAAGAAATGTGGTTAAGG 66 24 0 41.67 59.360 4.00 4.00 4.640 309 GCACTGAAGACCTTCCCTAATAC 144 23 0 47.83 58.358 6.00 1.00 4.642 310 CCTAAGAAATGTGGTTAAGGGA 70 22 0 40.91 57.332 4.00 0.00 4.668 311 TCCCTAAGAAATGTGGTTAAGG 68 22 0 40.91 57.332 4.00 4.00 4.668 312 AAGCTAAGACATTGTGGGTGTACC 169 24 0 45.83 60.673 5.00 4.00 4.673 313 GTGTACACACTGAACACGTTCCC 49 23 0 52.17 61.688 8.00 2.00 4.688 314 CATGTACACACTGAAGATGTTCCC 99 24 0 45.83 60.700 6.00 2.00 4.700 315 CCATGTACACACTGAAGATGTTCC 98 24 0 45.83 60.700 6.00 4.00 4.700 316 GAACACGTTCCCTAAGAAATGT 60 22 0 40.91 57.291 6.00 6.00 4.709 317 ACTGAACACGTTCCCTAAGAAAT 57 23 0 39.13 58.273 6.00 2.00 4.727 318 TGAAGATGTTCCCTAAGAATGTG 110 23 0 39.13 58.251 6.00 0.00 4.749 319 AAGATGTTCCCTAAGAATGTGG 112 22 0 40.91 57.236 4.00 0.00 4.764 320 CTGAACACGTTCCCTAAGAAATGT 58 24 0 41.67 60.767 6.00 6.00 4.767 321 ACTGAACACGTTCCCTAAGAAATG 57 24 0 41.67 60.767 6.00 4.00 4.767 322 CACTGAACACGTTCCCTAAGAAAT 56 24 0 41.67 60.767 6.00 2.00 4.767 323 CAAGGGAATGCACTGAAGACCT 135 22 0 50.00 62.786 4.00 2.00 4.786 324 ACACACTGAAGATGTTCCCTAAGA 104 24 0 41.67 59.212 6.00 1.00 4.788 325 ATGTTCCCTAAGAATGTGGCCA 115 22 0 45.45 62.791 6.00 6.00 4.791 326 CTGAAGATGTTCCCTAAGAATGTG 109 24 0 41.67 59.196 6.00 0.00 4.804 327 CACTGAAGATGTTCCCTAAGAATG 107 24 0 41.67 59.196 6.00 0.00 4.804 328 TGTACACACTGAACACGTTCCCT 50 23 0 47.83 61.806 6.00 4.00 4.806 329 TAAGAAATGTGGTTAAGGGACC 72 22 0 40.91 57.190 4.00 3.00 4.810 330 GAATGCACTGAAGACCTTCCCTA 140 23 0 47.83 61.828 6.00 2.00 4.828 331 AAAAGCTAAGACATTGTGGGTGTA 167 24 0 37.50 59.162 5.00 2.00 4.838 332 TACAAAAGCTAAGACATTGTGGGT 164 24 0 37.50 59.162 5.00 0.00 4.838 333 GCCATGTACACACTGAAGATGTTC 97 24 0 45.83 60.841 6.00 6.00 4.841 334 TGCACTGAAGACCTTCCC 143 18 0 55.56 57.158 6.00 2.00 4.842 335 CTAAGAAATGTGGTTAAGGGACC 71 23 0 43.48 58.153 4.00 3.00 4.847 336 CCTAAGAAATGTGGTTAAGGGAC 70 23 0 43.48 58.153 4.00 1.00 4.847 337 GCACTGAAGACCTTCCCTAATACA 144 24 0 45.83 60.860 6.00 0.00 4.860 338 TGCACTGAAGACCTTCCCTAATAC 143 24 0 45.83 60.860 6.00 1.00 4.860 339 GAAGACTTCCCTACATACAGCCAA 13 24 0 45.83 60.860 7.00 2.00 4.860 340 CAGCCAAGGGACTGCCAC 30 18 0 66.67 62.888 3.00 1.00 4.888 341 ACAGCCAAGGGACTGCCA 29 18 0 61.11 62.888 3.00 3.00 4.888 342 TTCCCTACATACAGCCAAGGGA 19 22 0 50.00 62.893 5.00 0.00 4.893 343 CATTGTGGGTGTACCACACACTT 178 23 0 47.83 61.918 8.00 6.00 4.918 344 TAAGACATTGTGGGTGTACCACAC 173 24 0 45.83 60.969 6.00 6.00 4.969 345 TAAGAATGTGGCCAAGGG 123 18 0 50.00 57.022 6.00 1.00 4.978 346 CCCTAAGAATGTGGCCAA 120 18 0 50.00 57.022 6.00 4.00 4.978 347 ATGTACACACTGAAGATGTTCCC 100 23 0 43.48 57.976 6.00 2.00 5.024 348 TGTACACACTGAAGATGTTCCCTAA 101 25 0 40.00 59.975 6.00 1.00 5.025 349 ATGTACACACTGAAGATGTTCCCT 100 24 0 41.67 58.969 6.00 4.00 5.031 350 ACTGAAGATGTTCCCTAAGAATGTG 108 25 0 40.00 59.947 6.00 0.00 5.053 351 CACTGAAGATGTTCCCTAAGAATGT 107 25 0 40.00 59.947 6.00 1.00 5.053 352 ACACTGAAGATGTTCCCTAAGAATG 106 25 0 40.00 59.947 6.00 0.00 5.053 353 CACACTGAAGATGTTCCCTAAGAAT 105 25 0 40.00 59.947 6.00 2.00 5.053 354 CACTGAAGACCTTCCCTAATACAAA 145 25 0 40.00 59.946 6.00 0.00 5.054 355 CTAAGACATTGTGGGTGTACCACA 172 24 0 45.83 61.086 6.00 6.00 5.086 356 GATCACACTCACTGAAGACTTCC 0 23 0 47.83 57.900 7.00 5.00 5.100 357 AAAGCTAAGACATTGTGGGTGTA 168 23 0 39.13 57.892 5.00 2.00 5.108 358 AAAAGCTAAGACATTGTGGGTGTAC 167 25 0 40.00 59.890 5.00 4.00 5.110 359 AATACAAAAGCTAAGACATTGTGGG 162 25 0 36.00 59.858 5.00 0.00 5.142 360 CACTCACTGAAGACTTCCCTACAT 5 24 0 45.83 58.854 7.00 2.00 5.146 361 GAAGACCTTCCCTAATACAAAAGC 149 24 0 41.67 58.814 6.00 2.00 5.186 362 CCTAAGAAATGTGGTTAAGGGACC 70 24 0 45.83 61.226 4.00 3.00 5.226 363 CCCTAAGAAATGTGGTTAAGGGAC 69 24 0 45.83 61.226 4.00 1.00 5.226 364 CACTGAAGACCTTCCCTAATACAA 145 24 0 41.67 58.759 6.00 0.00 5.241 365 ACACTGAACACGTTCCCTAAGAAA 55 24 0 41.67 61.267 6.00 4.00 5.267 366 CGCCATGTACACACTGAAGATGT 96 23 0 47.83 62.286 6.00 4.00 5.286 367 AAAGCTAAGACATTGTGGGTGTAC 168 24 0 41.67 58.699 5.00 4.00 5.301 368 TGTACACACTGAAGATGTTCCCTA 101 24 0 41.67 58.699 6.00 2.00 5.301 369 ATACAAAAGCTAAGACATTGTGGG 163 24 0 37.50 58.698 5.00 0.00 5.302 370 ACACTCACTGAAGACTTCCCTACAT 4 25 0 44.00 59.643 7.00 2.00 5.357 371 GACATTGTGGGTGTACCACACAC 176 23 0 52.17 62.364 8.00 8.00 5.364 372 GAAGACCTTCCCTAATACAAAAGCT 149 25 0 40.00 59.629 6.00 4.00 5.371 373 CACGTTCCCTAAGAAATGTGGTTA 63 24 0 41.67 61.414 8.00 2.00 5.414 374 ACACACTGAAGATGTTCCCTAAGAA 104 25 0 40.00 60.434 6.00 2.00 5.434 375 GATCACACTCACTGAAGACTTCCC 0 24 0 50.00 61.444 7.00 3.00 5.444 376 AGACATTGTGGGTGTACCACACA 175 23 0 47.83 62.479 8.00 8.00 5.479 377 AATGCACTGAAGACCTTCCCTAAT 141 24 0 41.67 61.502 6.00 2.00 5.502 378 TACCACACACTTCCTACAGCTGTG 189 24 0 50.00 61.532 8.00 6.00 5.532 379 TGTACCACACACTTCCTACAGCTG 187 24 0 50.00 61.532 6.00 6.00 5.532 380 TGAAGATGTTCCCTAAGAATGTGG 110 24 0 41.67 61.537 6.00 0.00 5.537 381 CACTGAAGACCTTCCCTAATACA 145 23 0 43.48 57.450 6.00 0.00 5.550 382 ATACAAAAGCTAAGACATTGTGGGT 163 25 0 36.00 59.432 5.00 1.00 5.568 383 ATCACACTCACTGAAGACTTCCCTA 1 25 0 44.00 60.571 7.00 3.00 5.571 384 CACTGAAGACTTCCCTACATACAGC 9 25 0 48.00 60.575 7.00 2.00 5.575 385 ACACACTGAAGATGTTCCCTAAG 104 23 0 43.48 57.416 6.00 3.00 5.584 386 CAAAAGCTAAGACATTGTGGGTGT 166 24 0 41.67 61.607 5.00 0.00 5.607 387 ACAAAAGCTAAGACATTGTGGGTG 165 24 0 41.67 61.607 5.00 0.00 5.607 388 AAGCTAAGACATTGTGGGTGTAC 169 23 0 43.48 57.388 5.00 4.00 5.612 389 TTCCCTAAGAAATGTGGTTAAGGG 67 24 0 41.67 61.631 4.00 4.00 5.631 390 AGATGTTCCCTAAGAATGTGGCC 113 23 0 47.83 62.687 4.00 4.00 5.687 391 TCCTACAGCTGTGGGAAAATGAG 200 23 0 47.83 62.697 12.00 0.00 5.697 392 ACTTCCCTACATACAGCCAAGGG 17 23 0 52.17 62.697 4.00 1.00 5.697 393 CGTGTACACACTGAACACGTTCC 48 23 0 52.17 62.750 9.00 4.00 5.750 394 CCGTGTACACACTGAACACGTTC 47 23 0 52.17 62.750 9.00 6.00 5.750 395 TGTACACACTGAACACGTTCCCTA 50 24 0 45.83 61.750 6.00 2.00 5.750 396 TACACACTGAAGATGTTCCCTAAGA 103 25 0 40.00 59.249 6.00 1.00 5.751 397 CTGAAGACTTCCCTACATACAGC 11 23 0 47.83 57.219 7.00 2.00 5.781 398 AAGACCTTCCCTAATACAAAAGC 150 23 0 39.13 57.202 4.00 2.00 5.798 399 GTGGGTGTACCACACACTTCCTAC 182 24 0 54.17 61.849 8.00 0.00 5.849 400 CATGTACACACTGAAGATGTTCC 99 23 0 43.48 57.126 6.00 4.00 5.874 401 CCATGTACACACTGAAGATGTTC 98 23 0 43.48 57.126 6.00 6.00 5.874 402 GCTAAGACATTGTGGGTGTACCAC 171 24 0 50.00 61.894 5.00 4.00 5.894 403 AAGACCTTCCCTAATACAAAAGCT 150 24 0 37.50 58.100 4.00 4.00 5.900 404 ACGTTCCCTAAGAAATGTGGTTAAG 64 25 0 40.00 60.930 6.00 2.00 5.930 405 ACTGAAGACTTCCCTACATACAGC 10 24 0 45.83 58.067 7.00 2.00 5.933 406 TGAAGACCTTCCCTAATACAAAA 148 23 0 34.78 57.052 6.00 1.00 5.948 407 ACACTGAAGATGTTCCCTAAGAA 106 23 0 39.13 57.049 6.00 2.00 5.951 408 GGGTGTACCACACACTTCCTACAG 184 24 0 54.17 61.959 5.00 2.00 5.959 409 ATGTACACACTGAAGATGTTCCCTA 100 25 0 40.00 59.015 6.00 2.00 5.985 410 AGCTAAGACATTGTGGGTGTACCA 170 24 0 45.83 62.000 5.00 2.00 6.000 411 AGACTTCCCTACATACAGCCAAGG 15 24 0 50.00 62.003 4.00 2.00 6.003 412 TGAAGACCTTCCCTAATACAAAAG 148 24 0 37.50 57.993 6.00 3.00 6.007 413 CTGAAGACCTTCCCTAATACAAAA 147 24 0 37.50 57.993 6.00 1.00 6.007 414 GTACACACTGAAGATGTTCCCTAAGA 102 26 0 42.31 59.993 6.00 1.00 6.007 415 TCACTGAAGACTTCCCTACATACA 8 24 0 41.67 57.947 7.00 0.00 6.053 416 ATGCACTGAAGACCTTCCCTAATAC 142 25 0 44.00 61.054 6.00 1.00 6.054 417 TCACACTCACTGAAGACTTCCCTAC 2 25 0 48.00 61.088 7.00 1.00 6.088 418 GGTGTACCACACACTTCCTACAGC 185 24 0 54.17 62.095 5.00 2.00 6.095 419 TCCCTAAGAAATGTGGTTAAGGGA 68 24 0 41.67 62.095 6.00 6.00 6.095 420 TCACTGAAGACTTCCCTACATACAG 8 25 0 44.00 58.904 7.00 2.00 6.096 421 CTCACTGAAGACTTCCCTACATACA 7 25 0 44.00 58.904 7.00 0.00 6.096 422 CACTCACTGAAGACTTCCCTACATA 5 25 0 44.00 58.904 7.00 2.00 6.096 423 TGAAGACCTTCCCTAATACAAAAGC 148 25 0 40.00 61.112 6.00 2.00 6.112 424 ACACGTTCCCTAAGAAATGTGGTT 62 24 0 41.67 62.116 8.00 4.00 6.116 425 AACACGTTCCCTAAGAAATGTGGT 61 24 0 41.67 62.116 8.00 8.00 6.116 426 CTAAGAAATGTGGTTAAGGGACCG 71 24 0 45.83 62.125 4.00 2.00 6.125 427 TAATACAAAAGCTAAGACATTGTGGG 161 26 0 34.62 59.872 5.00 0.00 6.128 428 CTGAAGACCTTCCCTAATACAAAAG 147 25 0 40.00 58.861 6.00 3.00 6.139 429 ACACACTGAACACGTTCCCTAAGA 53 24 0 45.83 62.186 6.00 1.00 6.186 430 TGAAGACTTCCCTACATACAGCCA 12 24 0 45.83 62.191 7.00 0.00 6.191 431 GTACACACTGAACACGTTCCCTAAG 51 25 0 48.00 61.201 6.00 3.00 6.201 432 ATGTACACACTGAAGATGTTCCCTAA 100 26 0 38.46 60.216 6.00 1.00 6.216 433 CTCACTGAAGACTTCCCTACATACAG 7 26 0 46.15 59.782 7.00 2.00 6.218 434 ACTGAAGACTTCCCTACATACAGCC 10 25 0 48.00 61.224 7.00 1.00 6.224 435 ACTGAAGACCTTCCCTAATACAAAA 146 25 0 36.00 58.743 6.00 1.00 6.257 436 GAAGATGTTCCCTAAGAATGTGGC 111 24 0 45.83 62.298 6.00 2.00 6.298 437 ACTCACTGAAGACTTCCCTACATACA 6 26 0 42.31 59.665 7.00 0.00 6.335 438 CACTCACTGAAGACTTCCCTACATAC 5 26 0 46.15 59.665 7.00 0.00 6.335 439 ACACTCACTGAAGACTTCCCTACATA 4 26 0 42.31 59.665 7.00 2.00 6.335 440 GACCTTCCCTAATACAAAAGCTAAGA 152 26 0 38.46 59.649 4.00 1.00 6.351 441 GAAGACCTTCCCTAATACAAAAGCTA 149 26 0 38.46 59.649 6.00 2.00 6.351 442 CCTTCCCTAATACAAAAGCTAAGACA 154 26 0 38.46 60.381 4.00 0.00 6.381 443 TGAACACGTTCCCTAAGAAATGTG 59 24 0 41.67 62.411 8.00 8.00 6.411 444 TACACACTGAAGATGTTCCCTAAGAA 103 26 0 38.46 60.429 6.00 2.00 6.429 445 ACTGAACACGTTCCCTAAGAAATGT 57 25 0 40.00 61.443 6.00 6.00 6.443 446 ACACTGAACACGTTCCCTAAGAAAT 55 25 0 40.00 61.443 6.00 2.00 6.443 447 ACTGAAGACCTTCCCTAATACAAAAG 146 26 0 38.46 59.556 6.00 3.00 6.444 448 AATGCACTGAAGACCTTCCCTAATA 141 25 0 40.00 61.463 6.00 3.00 6.463 449 ACTGAAGACCTTCCCTAATACAAA 146 24 0 37.50 57.525 6.00 0.00 6.475 450 TACACACTGAAGATGTTCCCTAAG 103 24 0 41.67 57.518 6.00 3.00 6.482 451 GTGTACACACTGAACACGTTCCCT 49 24 0 50.00 62.520 8.00 4.00 6.520 452 AATACAAAAGCTAAGACATTGTGGGT 162 26 0 34.62 60.524 5.00 1.00 6.524 453 CATGTACACACTGAAGATGTTCCCT 99 25 0 44.00 61.548 6.00 4.00 6.548 454 ACTGAAGATGTTCCCTAAGAATGT 108 24 0 37.50 57.442 6.00 1.00 6.558 455 ACACTGAAGATGTTCCCTAAGAAT 106 24 0 37.50 57.442 6.00 2.00 6.558 456 CAAAAGCTAAGACATTGTGGGTGTA 166 25 0 40.00 61.563 5.00 2.00 6.563 457 TACAAAAGCTAAGACATTGTGGGTG 164 25 0 40.00 61.563 5.00 0.00 6.563 458 CCTTCCCTAATACAAAAGCTAAGA 154 24 0 37.50 57.427 4.00 1.00 6.573 459 CACACTGAACACGTTCCCTAAGAA 54 24 0 45.83 62.619 6.00 2.00 6.619 460 GTACACACTGAAGATGTTCCCTAA 102 24 0 41.67 57.376 6.00 1.00 6.624 461 ACATTGTGGGTGTACCACACACTT 177 24 0 45.83 62.627 8.00 6.00 6.627 462 GAAGACTTCCCTACATACAGCCAAG 13 25 0 48.00 61.639 7.00 4.00 6.639 463 ACACTGAAGATGTTCCCTAAGAATGT 106 26 0 38.46 60.648 6.00 1.00 6.648 464 ACACACTGAAGATGTTCCCTAAGAAT 104 26 0 38.46 60.648 6.00 2.00 6.648 465 GTACACACTGAAGATGTTCCCTAAG 102 25 0 44.00 58.344 6.00 3.00 6.656 466 GACCTTCCCTAATACAAAAGCTAA 152 24 0 37.50 57.300 4.00 1.00 6.700 467 AAGACCTTCCCTAATACAAAAGCTAA 150 26 0 34.62 59.264 4.00 1.00 6.736 468 AAAGCTAAGACATTGTGGGTGTACC 168 25 0 44.00 61.766 5.00 4.00 6.766 469 TCCCTAATACAAAAGCTAAGACATTG 157 26 0 34.62 59.231 4.00 3.00 6.769 470 CTAAGACATTGTGGGTGTACCACAC 172 25 0 48.00 61.794 6.00 6.00 6.794 471 TGTACACACTGAAGATGTTCCCTAAG 101 26 0 42.31 60.811 6.00 3.00 6.811 472 CCTTCCCTAATACAAAAGCTAAGAC 154 25 0 40.00 58.167 4.00 1.00 6.833 473 ACCTTCCCTAATACAAAAGCTAAGA 153 25 0 36.00 58.167 4.00 1.00 6.833 474 GACCTTCCCTAATACAAAAGCTAAG 152 25 0 40.00 58.167 4.00 3.00 6.833 475 AGACCTTCCCTAATACAAAAGCTAA 151 25 0 36.00 58.167 4.00 1.00 6.833 476 AAGACCTTCCCTAATACAAAAGCTA 150 25 0 36.00 58.167 4.00 2.00 6.833 477 CACTGAAGACTTCCCTACATACAG 9 24 0 45.83 57.151 7.00 2.00 6.849 478 GAATGCACTGAAGACCTTCCCTAA 140 24 0 45.83 62.910 6.00 1.00 6.910 479 GCACTGAAGACCTTCCCTAATACAA 144 25 0 44.00 61.945 6.00 0.00 6.945 480 CACACTCACTGAAGACTTCCCTACA 3 25 0 48.00 61.960 7.00 1.00 6.960 481 AAGACCTTCCCTAATACAAAAGCTAAG 150 27 0 37.04 59.997 4.00 3.00 7.003 482 CACTGAAGACCTTCCCTAATACAAAA 145 26 0 38.46 61.028 6.00 1.00 7.028 483 AGACCTTCCCTAATACAAAAGCTAAG 151 26 0 38.46 58.971 4.00 3.00 7.029 484 ACACGTTCCCTAAGAAATGTGGTTA 62 25 0 40.00 62.057 8.00 2.00 7.057 485 TCCCTAATACAAAAGCTAAGACATTGT 157 27 0 33.33 59.891 5.00 5.00 7.109 486 TACACACTGAACACGTTCCCTAAGA 52 25 0 44.00 62.121 6.00 1.00 7.121 487 ACCTTCCCTAATACAAAAGCTAAGAC 153 26 0 38.46 58.862 4.00 1.00 7.138 488 GTACCACACACTTCCTACAGCTGTG 188 25 0 52.00 62.233 8.00 6.00 7.233 489 TGTACCACACACTTCCTACAGCTGT 187 25 0 48.00 62.233 8.00 8.00 7.233 490 GTGTACCACACACTTCCTACAGCTG 186 25 0 52.00 62.233 6.00 6.00 7.233 491 GTTCCCTAAGAAATGTGGTTAAGGG 66 25 0 44.00 62.241 4.00 4.00 7.241 492 ACAAAAGCTAAGACATTGTGGGTGT 165 25 0 40.00 62.258 5.00 0.00 7.258 493 GATCACACTCACTGAAGACTTCCCT 0 25 0 48.00 62.262 7.00 5.00 7.262 494 TTCCCTAATACAAAAGCTAAGACATTG 156 27 0 33.33 60.279 4.00 3.00 7.279 495 ATCACACTCACTGAAGACTTCCCTAC 1 26 0 46.15 61.279 7.00 1.00 7.279 496 GACCTTCCCTAATACAAAAGCTAAGAC 152 27 0 40.74 60.283 4.00 1.00 7.283 497 CCCTAATACAAAAGCTAAGACATTG 158 25 0 36.00 57.709 4.00 3.00 7.291 498 CTGAAGATGTTCCCTAAGAATGTGG 109 25 0 44.00 62.299 6.00 0.00 7.299 499 ACACTCACTGAAGACTTCCCTACATAC 4 27 0 44.44 60.375 7.00 0.00 7.375 500 AGACCTTCCCTAATACAAAAGCTAAGA 151 27 0 37.04 60.382 4.00 1.00 7.382 501 GTGTACACACTGAACACGTTCCCTA 49 25 0 48.00 62.441 8.00 2.00 7.441 502 CACGTTCCCTAAGAAATGTGGTTAA 63 25 0 40.00 62.450 8.00 4.00 7.450 503 ACTCACTGAAGACTTCCCTACATACAG 6 27 0 44.44 60.484 7.00 2.00 7.484 504 CATGTACACACTGAAGATGTTCCCTA 99 26 0 42.31 61.505 6.00 2.00 7.505 505 CTTCCCTAATACAAAAGCTAAGACA 155 25 0 36.00 57.487 4.00 0.00 7.513 506 TAATACAAAAGCTAAGACATTGTGGGT 161 27 0 33.33 60.517 5.00 1.00 7.517 507 CCCTAATACAAAAGCTAAGACATTGT 158 26 0 34.62 58.432 5.00 5.00 7.568 508 CCTTCCCTAATACAAAAGCTAAGACAT 154 27 0 37.04 60.573 4.00 2.00 7.573 509 CTAATACAAAAGCTAAGACATTGTGGG 160 27 0 37.04 60.616 5.00 0.00 7.616 510 CCTAATACAAAAGCTAAGACATTGTGG 159 27 0 37.04 60.616 5.00 1.00 7.616 511 CCCTAATACAAAAGCTAAGACATTGTG 158 27 0 37.04 60.616 5.00 3.00 7.616 512 TACACACTGAAGATGTTCCCTAAGAAT 103 27 0 37.04 60.637 6.00 2.00 7.637 513 AGCTAAGACATTGTGGGTGTACCAC 170 25 0 48.00 62.652 5.00 4.00 7.652 514 GAAGACCTTCCCTAATACAAAAGCTAA 149 27 0 37.04 60.660 6.00 1.00 7.660 515 TCCCTAAGAAATGTGGTTAAGGGAC 68 25 0 44.00 62.702 6.00 2.00 7.702 516 ATACAAAAGCTAAGACATTGTGGGTG 163 26 0 38.46 61.720 5.00 0.00 7.720 517 TGAAGACCTTCCCTAATACAAAAGCT 148 26 0 38.46 61.823 6.00 4.00 7.823 518 CTGAAGACCTTCCCTAATACAAAAGC 147 26 0 42.31 61.823 6.00 2.00 7.823 519 ACTCACTGAAGACTTCCCTACATAC 6 25 0 44.00 57.154 7.00 0.00 7.846 520 GGTGTACCACACACTTCCTACAGCT 185 25 0 52.00 62.860 5.00 4.00 7.860 521 TGTACACACTGAACACGTTCCCTAA 50 25 0 44.00 62.865 6.00 1.00 7.865 522 TTCCCTAATACAAAAGCTAAGACATT 156 26 0 30.77 58.116 4.00 3.00 7.884 523 CTGAAGACTTCCCTACATACAGCCA 11 25 0 48.00 62.932 7.00 0.00 7.932 524 ACCTTCCCTAATACAAAAGCTAAGACA 153 27 0 37.04 60.995 4.00 0.00 7.995 525 ATGTACACACTGAAGATGTTCCCTAAG 100 27 0 40.74 61.005 6.00 3.00 8.005 526 AATGCACTGAAGACCTTCCCTAATAC 141 26 0 42.31 62.082 6.00 1.00 8.082 527 CTTCCCTAATACAAAAGCTAAGACATT 155 27 0 33.33 58.903 4.00 3.00 8.097 528 GTACACACTGAAGATGTTCCCTAAGAA 102 27 0 40.74 61.101 6.00 2.00 8.101 529 CACACTCACTGAAGACTTCCCTACAT 3 26 0 46.15 62.110 7.00 2.00 8.110 530 TCACTGAAGACTTCCCTACATACAGC 8 26 0 46.15 62.145 7.00 2.00 8.145 531 CTTCCCTAATACAAAAGCTAAGACAT 155 26 0 34.62 57.811 4.00 2.00 8.189 532 GATCACACTCACTGAAGACTTCCCTA 0 26 0 46.15 62.195 7.00 3.00 8.195 533 CAAAAGCTAAGACATTGTGGGTGTAC 166 26 0 42.31 62.195 5.00 4.00 8.195 534 ACAAAAGCTAAGACATTGTGGGTGTA 165 26 0 38.46 62.195 5.00 2.00 8.195 535 TACAAAAGCTAAGACATTGTGGGTGT 164 26 0 38.46 62.195 5.00 0.00 8.195 536 CTAATACAAAAGCTAAGACATTGTGG 160 26 0 34.62 57.766 5.00 1.00 8.234 537 CCTAATACAAAAGCTAAGACATTGTG 159 26 0 34.62 57.766 5.00 3.00 8.234 538 CACTGAAGATGTTCCCTAAGAATGTG 107 26 0 42.31 62.327 6.00 0.00 8.327 539 CACACTGAAGATGTTCCCTAAGAATG 105 26 0 42.31 62.327 6.00 0.00 8.327 540 CACTGAAGACCTTCCCTAATACAAAAG 145 27 0 40.74 61.738 6.00 3.00 8.738 541 GTACACACTGAACACGTTCCCTAAGA 51 26 0 46.15 62.761 6.00 1.00 8.761 542 AAAAGCTAAGACATTGTGGGTGTACC 167 26 0 42.31 62.764 5.00 4.00 8.764 543 TGAAGACCTTCCCTAATACAAAAGCTA 148 27 0 37.04 61.778 6.00 2.00 8.778 544 GTGTACCACACACTTCCTACAGCTGT 186 26 0 50.00 62.885 8.00 8.00 8.885 545 ACTGAAGATGTTCCCTAAGAATGTGG 108 26 0 42.31 62.911 6.00 0.00 8.911 546 GAATGCACTGAAGACCTTCCCTAATA 140 26 0 42.31 62.924 6.00 3.00 8.924 547 GCACTGAAGACCTTCCCTAATACAAA 144 26 0 42.31 62.934 6.00 0.00 8.934 548 CACTCACTGAAGACTTCCCTACATACA 5 27 0 44.44 62.050 7.00 0.00 9.050 549 CACACTCACTGAAGACTTCCCTACATA 3 27 0 44.44 62.050 7.00 2.00 9.050 550 TACAAAAGCTAAGACATTGTGGGTGTA 164 27 0 37.04 62.136 5.00 2.00 9.136 551 ATACAAAAGCTAAGACATTGTGGGTGT 163 27 0 37.04 62.320 5.00 0.00 9.320 552 TGTACACACTGAAGATGTTCCCTAAGA 101 27 0 40.74 62.355 6.00 1.00 9.355 553 ACTGAAGACCTTCCCTAATACAAAAGC 146 27 0 40.74 62.397 6.00 2.00 9.397 554 CTGAAGACCTTCCCTAATACAAAAGCT 147 27 0 40.74 62.483 6.00 4.00 9.483 555 CATGTACACACTGAAGATGTTCCCTAA 99 27 0 40.74 62.561 6.00 1.00 9.561 556 AATACAAAAGCTAAGACATTGTGGGTG 162 27 0 37.04 62.689 5.00 0.00 9.689 557 ACAAAAGCTAAGACATTGTGGGTGTAC 165 27 0 40.74 62.787 5.00 4.00 9.787 558 GATCACACTCACTGAAGACTTCCCTAC 0 27 0 48.15 62.829 7.00 1.00 9.829 559 CTCACTGAAGACTTCCCTACATACAGC 7 27 0 48.15 62.853 7.00 2.00 9.853 560 ACACTGAAGATGTTCCCTAAGAATGTG 106 27 0 40.74 62.934 6.00 0.00 9.934 561 CACACTGAAGATGTTCCCTAAGAATGT 105 27 0 40.74 62.934 6.00 1.00 9.934 562 ACACACTGAAGATGTTCCCTAAGAATG 104 27 0 40.74 62.934 6.00 0.00 9.934 primer3-2.3.6/test/primer_list_last/SP01-03.txt.rev0000664000077300007730000001001112231156244020640 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 GATCCTGCTGAAGCAAATGG 174 20 0 50.00 60.744 6.00 1.00 0.744 1 ATCCTGCTGAAGCAAATGGA 173 20 0 45.00 60.744 6.00 1.00 0.744 2 CTGAAGCAAATGGACGTGG 167 19 0 52.63 60.247 4.00 0.00 1.247 3 TCCTGCTGAAGCAAATGGAC 172 20 0 50.00 61.329 6.00 1.00 1.329 4 CTGCTGAAGCAAATGGACGT 170 20 0 50.00 61.375 6.00 4.00 1.375 5 GTCACCAAATGCACCCAGAC 102 20 0 55.00 61.396 4.00 1.00 1.396 6 TGCTGAAGCAAATGGACGT 169 19 0 47.37 60.406 6.00 4.00 1.406 7 GCTGAAGCAAATGGACGTG 168 19 0 52.63 60.406 4.00 2.00 1.406 8 TCCTGCTGAAGCAAATGGA 172 19 0 47.37 60.487 6.00 1.00 1.487 9 GTCACCAAATGCACCCAGA 102 19 0 52.63 60.527 4.00 0.00 1.527 10 TCACCAAATGCACCCAGAC 101 19 0 52.63 60.527 4.00 1.00 1.527 11 CTGCTGAAGCAAATGGACG 170 19 0 52.63 60.546 6.00 2.00 1.546 12 CCTGCTGAAGCAAATGGAC 171 19 0 52.63 59.384 6.00 1.00 1.616 13 ACCAAATGCACCCAGACAC 99 19 0 52.63 59.383 4.00 0.00 1.617 14 AGCAAATGGACGTGGCAT 163 18 0 50.00 60.080 4.00 4.00 2.080 15 ATCCTGCTGAAGCAAATGG 173 19 0 47.37 58.811 6.00 1.00 2.189 16 GAAGCAAATGGACGTGGC 165 18 0 55.56 60.210 4.00 2.00 2.210 17 CTGCGGTGTCACCAAATG 109 18 0 55.56 59.652 7.00 3.00 2.348 18 GTGTCACCAAATGCACCCAG 104 20 0 55.00 62.415 5.00 1.00 2.415 19 CACCAAATGCACCCAGACAC 100 20 0 55.00 62.415 4.00 0.00 2.415 20 TCACCAAATGCACCCAGA 101 18 0 50.00 59.577 4.00 0.00 2.423 21 GTGTCACCAAATGCACCCA 104 19 0 52.63 61.452 5.00 0.00 2.452 22 AAGCAAATGGACGTGGCAT 164 19 0 47.37 61.465 4.00 4.00 2.465 23 CGGTGTCACCAAATGCAC 106 18 0 55.56 59.492 7.00 2.00 2.508 24 TGCTGAAGCAAATGGACG 169 18 0 50.00 59.490 6.00 2.00 2.510 25 ATCCTGCTGAAGCAAATGGAC 173 21 0 47.62 61.530 6.00 1.00 2.530 26 CCTGCGGTGTCACCAAAT 110 18 0 55.56 60.534 7.00 3.00 2.534 27 TGTCACCAAATGCACCCA 103 18 0 50.00 60.535 4.00 0.00 2.535 28 TGTCACCAAATGCACCCAG 103 19 0 52.63 61.589 4.00 1.00 2.589 29 CACCAAATGCACCCAGACA 100 19 0 52.63 61.589 4.00 0.00 2.589 30 TGAAGCAAATGGACGTGG 166 18 0 50.00 59.165 4.00 0.00 2.835 31 CAAATGGACGTGGCATCC 161 18 0 55.56 60.915 4.00 3.00 2.915 32 AGCAAATGGACGTGGCATC 163 19 0 52.63 62.050 4.00 2.00 3.050 33 GCAAATGGACGTGGCATC 162 18 0 55.56 61.080 4.00 2.00 3.080 34 AAGCAAATGGACGTGGCA 164 18 0 50.00 61.248 4.00 3.00 3.248 35 GGTGTCACCAAATGCACCC 105 19 0 57.89 62.254 7.00 1.00 3.254 36 GATCCTGCTGAAGCAAATGGA 174 21 0 47.62 62.559 6.00 1.00 3.559 37 TCCTGCTGAAGCAAATGG 172 18 0 50.00 58.427 6.00 1.00 3.573 38 CCTGCTGAAGCAAATGGA 171 18 0 50.00 58.427 6.00 0.00 3.573 39 GTCACCAAATGCACCCAG 102 18 0 55.56 58.389 4.00 1.00 3.611 40 CACCAAATGCACCCAGAC 100 18 0 55.56 58.389 4.00 1.00 3.611 41 ACCAAATGCACCCAGACA 99 18 0 50.00 58.389 4.00 0.00 3.611 42 CTCTGCCTGCGGTGTCAC 115 18 0 66.67 61.691 5.00 5.00 3.691 43 GGTGTCACCAAATGCACC 105 18 0 55.56 58.218 7.00 3.00 3.782 44 GTGTCACCAAATGCACCC 104 18 0 55.56 58.218 5.00 0.00 3.782 45 GCTGAAGCAAATGGACGT 168 18 0 50.00 57.302 4.00 4.00 4.698 46 TGCGGTGTCACCAAATGC 108 18 0 55.56 62.807 7.00 2.00 4.807 47 GCGGTGTCACCAAATGCA 107 18 0 55.56 62.807 7.00 4.00 4.807 48 AAATGGACGTGGCATCCG 160 18 0 55.56 62.891 4.00 2.00 4.891 primer3-2.3.6/test/primer_list_last/included_test_w_middle.rev0000664000077300007730000006241112231156244023624 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 CACAACAGACACACACACACAA 186 22 0 45.45 59.121 1.00 0.00 2.879 1 CAACAGACACACACACACAACA 184 22 0 45.45 59.121 1.00 0.00 2.879 2 CACACACACAACACACAGTCAA 175 22 0 45.45 59.121 3.00 0.00 2.879 3 ACACAACAGACACACACACACAA 187 23 0 43.48 60.064 1.00 0.00 3.064 4 CACAACAGACACACACACACAAC 186 23 0 47.83 60.064 1.00 0.00 3.064 5 ACAACAGACACACACACACAACA 185 23 0 43.48 60.064 1.00 0.00 3.064 6 CAACAGACACACACACACAACAC 184 23 0 47.83 60.064 1.00 0.00 3.064 7 AACAGACACACACACACAACACA 183 23 0 43.48 60.064 1.00 0.00 3.064 8 ACACACACACAACACACAGTCAA 176 23 0 43.48 60.064 3.00 0.00 3.064 9 CACACACACAACACACAGTCAAG 175 23 0 47.83 60.208 3.00 0.00 3.208 10 CACACACAACACACAGTCAAGAA 173 23 0 43.48 59.744 3.00 0.00 3.256 11 AGACACACACACACAACACACAG 180 23 0 47.83 59.650 1.00 1.00 3.350 12 ACACAACAGACACACACACACA 187 22 0 45.45 58.514 1.00 0.00 3.486 13 ACAGACACACACACACAACACA 182 22 0 45.45 58.514 1.00 0.00 3.486 14 CAGACACACACACACAACACAC 181 22 0 50.00 58.514 1.00 0.00 3.486 15 AGACACACACACACAACACACA 180 22 0 45.45 58.514 1.00 0.00 3.486 16 GACACACACACACAACACACAG 179 22 0 50.00 58.514 1.00 1.00 3.486 17 CACACACACACAACACACAGTC 177 22 0 50.00 58.514 3.00 1.00 3.486 18 ACACACACACAACACACAGTCA 176 22 0 45.45 58.514 3.00 1.00 3.486 19 ACAGACACACACACACAACACAC 182 23 0 47.83 59.499 1.00 0.00 3.501 20 GACACACACACACAACACACAGT 179 23 0 47.83 59.499 3.00 3.00 3.501 21 ACACACACACACAACACACAGTC 178 23 0 47.83 59.499 3.00 1.00 3.501 22 CACAACAGACACACACACACA 186 21 0 47.62 57.434 1.00 0.00 3.566 23 CAGACACACACACACAACACA 181 21 0 47.62 57.434 1.00 0.00 3.566 24 CACACACACAACACACAGTCA 175 21 0 47.62 57.434 3.00 1.00 3.566 25 TGCACAATACCACACATGTTT 121 21 0 38.10 57.391 6.00 6.00 3.609 26 GACACACACACACAACACACA 179 21 0 47.62 57.244 1.00 0.00 3.756 27 CACACACAACACACAGTCAAGA 173 22 0 45.45 58.217 3.00 2.00 3.783 28 ACACACACAACACACAGTCAAGA 174 23 0 43.48 59.188 3.00 2.00 3.812 29 CACACAACACACAGTCAAGAAGC 171 23 0 47.83 60.832 3.00 2.00 3.832 30 CACAACACACAGTCAAGAAGCT 169 22 0 45.45 58.075 4.00 4.00 3.925 31 CACAGTCAAGAAGCTACATCCA 162 22 0 45.45 58.036 4.00 0.00 3.964 32 ACACAACACACAGTCAAGAAGCT 170 23 0 43.48 58.981 4.00 4.00 4.019 33 TACACAACAGACACACACACACAA 188 24 0 41.67 60.073 2.00 0.00 4.073 34 CACACAGTCAAGAAGCTACATCC 164 23 0 47.83 58.925 4.00 0.00 4.075 35 ACACAGTCAAGAAGCTACATCCA 163 23 0 43.48 58.925 4.00 0.00 4.075 36 TGCACAATACCACACATGTTTAA 121 23 0 34.78 58.925 6.00 4.00 4.075 37 ACACAACACACAGTCAAGAAGC 170 22 0 45.45 57.918 3.00 2.00 4.082 38 ATGCACAATACCACACATGTTT 122 22 0 36.36 57.811 6.00 6.00 4.189 39 ACACACAGTCAAGAAGCTACATCC 165 24 0 45.83 59.748 4.00 0.00 4.252 40 TGCACAATACCACACATGTTTAAC 121 24 0 37.50 59.733 6.00 5.00 4.267 41 GCACAATACCACACATGTTTAACA 120 24 0 37.50 59.733 7.00 7.00 4.267 42 TACACAACAGACACACACACACA 188 23 0 43.48 58.590 2.00 0.00 4.410 43 TGCACAATACCACACATGTTTA 121 22 0 36.36 57.504 6.00 4.00 4.496 44 GCTACATCCATACACAACACACA 150 23 0 43.48 58.499 2.00 0.00 4.501 45 AGCTACATCCATACACAACACACA 151 24 0 41.67 59.493 4.00 0.00 4.507 46 AGACACACACACACAACACACAGT 180 24 0 45.83 60.539 3.00 3.00 4.539 47 CACAGTCAAGAAGCTACATCCAT 162 23 0 43.48 58.408 4.00 2.00 4.592 48 ACACACACAACACACAGTCAAGAA 174 24 0 41.67 60.599 3.00 0.00 4.599 49 ACACACACACACAACACACAGT 178 22 0 45.45 57.352 3.00 3.00 4.648 50 CACAACACACATGTATATGCACAA 138 24 0 37.50 59.345 6.00 2.00 4.655 51 CAGACACACACACACAACACACA 181 23 0 47.83 61.701 1.00 0.00 4.701 52 CACACACACACAACACACAGTCA 177 23 0 47.83 61.701 3.00 1.00 4.701 53 CACACACAACACACAGTCAAGAAG 173 24 0 45.83 60.730 3.00 0.00 4.730 54 ACACAGTCAAGAAGCTACATCCAT 163 24 0 41.67 59.238 4.00 2.00 4.762 55 ATGCACAATACCACACATGTTTAA 122 24 0 33.33 59.233 6.00 4.00 4.767 56 CACAACACACAGTCAAGAAGCTA 169 23 0 43.48 58.160 4.00 2.00 4.840 57 CAACACACAGTCAAGAAGCTACA 167 23 0 43.48 58.160 4.00 2.00 4.840 58 GAAGCTACATCCATACACAACACA 153 24 0 41.67 59.091 4.00 0.00 4.909 59 ACACACACAACACACAGTCAAG 174 22 0 45.45 57.090 3.00 0.00 4.910 60 ACACAACAGACACACACACACAAC 187 24 0 45.83 60.929 1.00 0.00 4.929 61 ACAACAGACACACACACACAACAC 185 24 0 45.83 60.929 1.00 0.00 4.929 62 AACAGACACACACACACAACACAC 183 24 0 45.83 60.929 1.00 0.00 4.929 63 ACACAACACACAGTCAAGAAGCTA 170 24 0 41.67 59.030 4.00 2.00 4.970 64 CACAACACACAGTCAAGAAGCTAC 169 24 0 45.83 59.030 4.00 4.00 4.970 65 ACAACACACAGTCAAGAAGCTACA 168 24 0 41.67 59.030 4.00 2.00 4.970 66 AGAAGCTACATCCATACACAACACA 154 25 0 40.00 60.002 4.00 0.00 5.002 67 ATGCACAATACCACACATGTTTAAC 122 25 0 36.00 59.991 6.00 5.00 5.009 68 ATACACAACAGACACACACACACA 189 24 0 41.67 58.961 2.00 0.00 5.039 69 ACACACACACAACACACAGTCAAG 176 24 0 45.83 61.059 3.00 0.00 5.059 70 CACAACACACATGTATATGCACA 138 23 0 39.13 57.923 6.00 4.00 5.077 71 TATGCACAATACCACACATGTTT 123 23 0 34.78 57.903 6.00 6.00 5.097 72 ATGCACAATACCACACATGTTTA 122 23 0 34.78 57.903 6.00 4.00 5.097 73 GAAGCTACATCCATACACAACACAC 153 25 0 44.00 59.880 4.00 0.00 5.120 74 ACACAACACACATGTATATGCACAA 139 25 0 36.00 60.151 6.00 2.00 5.151 75 GCTACATCCATACACAACACACAT 150 24 0 41.67 58.842 2.00 2.00 5.158 76 ACACACAACACACAGTCAAGAAG 172 23 0 43.48 57.837 3.00 0.00 5.163 77 ACACAACACACAGTCAAGAAGCTAC 170 25 0 44.00 59.835 4.00 4.00 5.165 78 TCAAGAAGCTACATCCATACACAA 157 24 0 37.50 58.834 4.00 0.00 5.166 79 ACACAACACACATGTATATGCACA 139 24 0 37.50 58.828 6.00 4.00 5.172 80 AGCTACATCCATACACAACACACAT 151 25 0 40.00 59.772 4.00 2.00 5.228 81 CAACACACAGTCAAGAAGCTACATC 167 25 0 44.00 60.272 4.00 2.00 5.272 82 ATACACAACAGACACACACACACAA 189 25 0 40.00 60.344 2.00 0.00 5.344 83 ACATCCATACACAACACACATGT 147 23 0 39.13 57.646 6.00 6.00 5.354 84 CACAACACACATGTATATGCACAAT 138 25 0 36.00 59.637 6.00 2.00 5.363 85 GTCAAGAAGCTACATCCATACACAA 158 25 0 40.00 59.615 4.00 0.00 5.385 86 TCAAGAAGCTACATCCATACACAAC 157 25 0 40.00 59.615 4.00 0.00 5.385 87 CAAGAAGCTACATCCATACACAACA 156 25 0 40.00 60.458 4.00 0.00 5.458 88 CAACACACAGTCAAGAAGCTACAT 167 24 0 41.67 58.520 4.00 4.00 5.480 89 GACACACACACACAACACACAGTC 179 24 0 50.00 61.504 3.00 1.00 5.504 90 CACAGTCAAGAAGCTACATCCATA 162 24 0 41.67 58.476 4.00 2.00 5.524 91 CAGTCAAGAAGCTACATCCATACA 160 24 0 41.67 58.476 4.00 0.00 5.524 92 TCAAGAAGCTACATCCATACACA 157 23 0 39.13 57.467 4.00 0.00 5.533 93 GCACAATACCACACATGTTTAACAG 120 25 0 40.00 60.599 7.00 5.00 5.599 94 ACACACAACACACAGTCAAGAAGC 172 24 0 45.83 61.613 3.00 2.00 5.613 95 CACACAGTCAAGAAGCTACATCCA 164 24 0 45.83 61.619 4.00 0.00 5.619 96 GTCAAGAAGCTACATCCATACACA 158 24 0 41.67 58.339 4.00 0.00 5.661 97 ACAACACACAGTCAAGAAGCTACAT 168 25 0 40.00 59.333 4.00 4.00 5.667 98 CACACATGTATATGCACAATACCA 133 24 0 37.50 58.315 6.00 4.00 5.685 99 CACATGTATATGCACAATACCACA 131 24 0 37.50 58.315 6.00 2.00 5.685 100 CATGTATATGCACAATACCACACA 129 24 0 37.50 58.315 6.00 0.00 5.685 101 TGTATATGCACAATACCACACATG 127 24 0 37.50 58.315 6.00 4.00 5.685 102 ACACAGTCAAGAAGCTACATCCATA 163 25 0 40.00 59.274 4.00 2.00 5.726 103 CACAGTCAAGAAGCTACATCCATAC 162 25 0 44.00 59.274 4.00 0.00 5.726 104 ACAGTCAAGAAGCTACATCCATACA 161 25 0 40.00 59.274 4.00 0.00 5.726 105 CAGTCAAGAAGCTACATCCATACAC 160 25 0 44.00 59.274 4.00 0.00 5.726 106 AGTCAAGAAGCTACATCCATACACA 159 25 0 40.00 59.274 4.00 0.00 5.726 107 CACACAACACACAGTCAAGAAGCT 171 24 0 45.83 61.730 4.00 4.00 5.730 108 TATGCACAATACCACACATGTTTAA 123 25 0 32.00 59.268 6.00 4.00 5.732 109 ATATGCACAATACCACACATGTTT 124 24 0 33.33 58.262 6.00 6.00 5.738 110 AAGCTACATCCATACACAACACACA 152 25 0 40.00 60.742 4.00 0.00 5.742 111 AAGCTACATCCATACACAACACA 152 23 0 39.13 57.257 4.00 0.00 5.743 112 AAGCTACATCCATACACAACACAC 152 24 0 41.67 58.137 4.00 0.00 5.863 113 CCATACACAACACACATGTATATGC 143 25 0 40.00 59.136 6.00 4.00 5.864 114 ACACACATGTATATGCACAATACCA 134 25 0 36.00 59.136 6.00 4.00 5.864 115 CACACATGTATATGCACAATACCAC 133 25 0 40.00 59.136 6.00 2.00 5.864 116 ACACATGTATATGCACAATACCACA 132 25 0 36.00 59.136 6.00 2.00 5.864 117 CACATGTATATGCACAATACCACAC 131 25 0 40.00 59.136 6.00 0.00 5.864 118 ACATGTATATGCACAATACCACACA 130 25 0 36.00 59.136 6.00 0.00 5.864 119 TGTATATGCACAATACCACACATGT 127 25 0 36.00 59.136 6.00 6.00 5.864 120 TACACAACAGACACACACACACAAC 188 25 0 44.00 60.904 2.00 0.00 5.904 121 GCACAATACCACACATGTTTAAC 120 23 0 39.13 57.060 6.00 5.00 5.940 122 AACACACAGTCAAGAAGCTACATCC 166 25 0 44.00 60.962 4.00 0.00 5.962 123 CAAGAAGCTACATCCATACACAA 156 23 0 39.13 57.036 4.00 0.00 5.964 124 TATACACAACAGACACACACACACA 190 25 0 40.00 59.013 4.00 0.00 5.987 125 TATGCACAATACCACACATGTTTAAC 123 26 0 34.62 60.001 6.00 5.00 6.001 126 TATGCACAATACCACACATGTTTA 123 24 0 33.33 57.988 6.00 4.00 6.012 127 ACACAGTCAAGAAGCTACATCCATAC 163 26 0 42.31 60.018 4.00 0.00 6.018 128 ACAGTCAAGAAGCTACATCCATACAC 161 26 0 42.31 60.018 4.00 0.00 6.018 129 CACAATACCACACATGTTTAACAGA 119 25 0 36.00 58.912 7.00 3.00 6.088 130 CAAGAAGCTACATCCATACACAAC 156 24 0 41.67 57.904 4.00 0.00 6.096 131 AAGAAGCTACATCCATACACAACA 155 24 0 37.50 57.904 4.00 0.00 6.096 132 ACACACATGTATATGCACAATACCAC 134 26 0 38.46 59.900 6.00 2.00 6.100 133 ACACATGTATATGCACAATACCACAC 132 26 0 38.46 59.900 6.00 0.00 6.100 134 CTACATCCATACACAACACACATG 149 24 0 41.67 57.896 4.00 4.00 6.104 135 TACACAACACACATGTATATGCACA 140 25 0 36.00 58.882 6.00 4.00 6.118 136 CACACACACAACACACAGTCAAGA 175 24 0 45.83 62.141 3.00 2.00 6.141 137 TACACAACACACATGTATATGCACAA 140 26 0 34.62 60.156 6.00 2.00 6.156 138 CTACATCCATACACAACACACATGT 149 25 0 40.00 58.761 6.00 6.00 6.239 139 TACATCCATACACAACACACATGT 148 24 0 37.50 57.747 6.00 6.00 6.253 140 ACATCCATACACAACACACATGTA 147 24 0 37.50 57.747 6.00 4.00 6.253 141 AAGAAGCTACATCCATACACAACAC 155 25 0 40.00 58.712 4.00 0.00 6.288 142 CACAATACCACACATGTTTAACAGAC 119 26 0 38.46 59.680 7.00 1.00 6.320 143 CACAACACACATGTATATGCACAATA 138 26 0 34.62 59.659 6.00 4.00 6.341 144 TATACACAACAGACACACACACACAA 190 26 0 38.46 60.342 4.00 0.00 6.342 145 GTCAAGAAGCTACATCCATACACAAC 158 26 0 42.31 60.343 4.00 0.00 6.343 146 CAACACACATGTATATGCACAATACC 136 26 0 38.46 60.346 6.00 4.00 6.346 147 AACACACATGTATATGCACAATACCA 135 26 0 34.62 60.346 6.00 4.00 6.346 148 TGTATATGCACAATACCACACATGTT 127 26 0 34.62 60.346 6.00 4.00 6.346 149 CATGTATATGCACAATACCACACAT 129 25 0 36.00 58.650 6.00 2.00 6.350 150 ATGTATATGCACAATACCACACATG 128 25 0 36.00 58.650 6.00 4.00 6.350 151 AACACACAGTCAAGAAGCTACATC 166 24 0 41.67 57.626 4.00 2.00 6.374 152 ACACAACACACATGTATATGCACAAT 139 26 0 34.62 60.393 6.00 2.00 6.393 153 AGTCAAGAAGCTACATCCATACACAA 159 26 0 38.46 60.455 4.00 0.00 6.455 154 ATATGCACAATACCACACATGTTTAA 124 26 0 30.77 59.536 6.00 4.00 6.464 155 GCTACATCCATACACAACACACATG 150 25 0 44.00 61.472 4.00 4.00 6.472 156 ACACACACAACACACAGTCAAGAAG 174 25 0 44.00 61.508 3.00 0.00 6.508 157 ACAGACACACACACACAACACACA 182 24 0 45.83 62.520 1.00 0.00 6.520 158 ACACACACACACAACACACAGTCA 178 24 0 45.83 62.520 3.00 1.00 6.520 159 ACATGTATATGCACAATACCACACAT 130 26 0 34.62 59.420 6.00 2.00 6.580 160 ATGTATATGCACAATACCACACATGT 128 26 0 34.62 59.420 6.00 6.00 6.580 161 AGAAGCTACATCCATACACAACAC 154 24 0 41.67 57.400 4.00 0.00 6.600 162 CATACACAACACACATGTATATGCA 142 25 0 36.00 58.378 6.00 6.00 6.622 163 CAGACACACACACACAACACACAG 181 24 0 50.00 62.641 1.00 1.00 6.641 164 TATATGCACAATACCACACATGTTT 125 25 0 32.00 58.331 6.00 6.00 6.669 165 ATATGCACAATACCACACATGTTTA 124 25 0 32.00 58.331 6.00 4.00 6.669 166 ATATACACAACAGACACACACACACA 191 26 0 38.46 59.329 4.00 0.00 6.671 167 CACACAACACACAGTCAAGAAGCTA 171 25 0 44.00 61.678 4.00 2.00 6.678 168 CACAACACACAGTCAAGAAGCTACA 169 25 0 44.00 61.678 4.00 2.00 6.678 169 AGAAGCTACATCCATACACAACACAC 154 26 0 42.31 60.727 4.00 0.00 6.727 170 CATCCATACACAACACACATGTAT 146 24 0 37.50 57.241 6.00 6.00 6.759 171 CACACACACACAACATACACTCTAGA 241 26 0 42.31 59.211 6.00 6.00 6.789 172 CACACACACAACATACACTCTAGACA 239 26 0 42.31 59.211 6.00 2.00 6.789 173 CACACACAACATACACTCTAGACACA 237 26 0 42.31 59.211 6.00 0.00 6.789 174 CACACAGTCAAGAAGCTACATCCAT 164 25 0 44.00 61.791 4.00 2.00 6.791 175 CATACACAACACACATGTATATGCAC 142 26 0 38.46 59.190 6.00 4.00 6.810 176 ATACACAACACACATGTATATGCACA 141 26 0 34.62 59.190 6.00 4.00 6.810 177 TCCATACACAACACACATGTATATGC 144 26 0 38.46 60.811 6.00 4.00 6.811 178 CACAATACCACACATGTTTAACAG 119 24 0 37.50 57.140 7.00 5.00 6.860 179 ACATCCATACACAACACACATGTAT 147 25 0 36.00 58.124 6.00 6.00 6.876 180 ACACACACACACAACATACACTCT 242 24 0 41.67 57.096 2.00 1.00 6.904 181 GTATATGCACAATACCACACATGTTT 126 26 0 34.62 59.095 6.00 6.00 6.905 182 TCTACACACACACACAACATACACTC 245 26 0 42.31 59.077 2.00 0.00 6.923 183 AAGCTACATCCATACACAACACACAT 152 26 0 38.46 60.948 4.00 2.00 6.948 184 TATATGCACAATACCACACATGTT 125 24 0 33.33 57.002 6.00 4.00 6.998 185 TCTACACACACACACAACATACACTCT 245 27 0 40.74 60.001 2.00 1.00 7.001 186 ACACACACACACAACATACACTCTAGA 242 27 0 40.74 60.001 6.00 6.00 7.001 187 CACACACACACAACATACACTCTAGAC 241 27 0 44.44 60.001 6.00 4.00 7.001 188 ACACACACACAACATACACTCTAGACA 240 27 0 40.74 60.001 6.00 2.00 7.001 189 CACACACACAACATACACTCTAGACAC 239 27 0 44.44 60.001 6.00 0.00 7.001 190 ACACACACAACATACACTCTAGACACA 238 27 0 40.74 60.001 6.00 0.00 7.001 191 ACAACACACAGTCAAGAAGCTACATC 168 26 0 42.31 61.001 4.00 2.00 7.001 192 ATACACAACAGACACACACACACAAC 189 26 0 42.31 61.121 2.00 0.00 7.121 193 CACACACAACATACACTCTAGACACAG 237 27 0 44.44 60.122 6.00 1.00 7.122 194 CATGTATATGCACAATACCACACATG 129 26 0 38.46 61.147 6.00 4.00 7.147 195 AACACACATGTATATGCACAATACC 135 25 0 36.00 57.851 6.00 4.00 7.149 196 GTATATGCACAATACCACACATGTT 126 25 0 36.00 57.851 6.00 4.00 7.149 197 CAAGAAGCTACATCCATACACAACAC 156 26 0 42.31 61.157 4.00 0.00 7.157 198 AAGAAGCTACATCCATACACAACACA 155 26 0 38.46 61.157 4.00 0.00 7.157 199 TACATCCATACACAACACACATGTA 148 25 0 36.00 57.841 6.00 4.00 7.159 200 CTACATCCATACACAACACACATGTA 149 26 0 38.46 58.815 6.00 4.00 7.185 201 ATATGCACAATACCACACATGTTTAAC 124 27 0 33.33 60.227 6.00 5.00 7.227 202 CATCCATACACAACACACATGTATATG 146 27 0 37.04 60.230 6.00 6.00 7.230 203 AACACACGTGTATATACACAACAGACA 202 27 0 37.04 59.748 12.00 1.00 7.252 204 TGCACAATACCACACATGTTTAACA 121 25 0 36.00 62.264 7.00 7.00 7.264 205 ACACACAGTCAAGAAGCTACATCCA 165 25 0 44.00 62.322 4.00 0.00 7.322 206 CACACGTGTATATACACAACAGACA 200 25 0 40.00 57.664 12.00 1.00 7.336 207 CACGTGTATATACACAACAGACACA 198 25 0 40.00 57.664 12.00 1.00 7.336 208 CGTGTATATACACAACAGACACACA 196 25 0 40.00 57.664 12.00 0.00 7.336 209 ATACACAACACACATGTATATGCACAA 141 27 0 33.33 60.389 6.00 2.00 7.389 210 TACACAACACACATGTATATGCACAAT 140 27 0 33.33 60.389 6.00 2.00 7.389 211 ACACAACACACATGTATATGCACAATA 139 27 0 33.33 60.389 6.00 4.00 7.389 212 CACAACACACATGTATATGCACAATAC 138 27 0 37.04 60.389 6.00 6.00 7.389 213 AGACACACACACACAACACACAGTC 180 25 0 48.00 62.414 3.00 1.00 7.414 214 TATATGCACAATACCACACATGTTTAA 125 27 0 29.63 59.560 6.00 4.00 7.440 215 ACACACAACACACAGTCAAGAAGCT 172 25 0 44.00 62.443 4.00 4.00 7.443 216 GATCTACACACACACACAACATACA 247 25 0 40.00 57.548 4.00 0.00 7.452 217 ACACACGTGTATATACACAACAGACA 201 26 0 38.46 58.508 12.00 1.00 7.492 218 CACACGTGTATATACACAACAGACAC 200 26 0 42.31 58.508 12.00 3.00 7.492 219 ACACGTGTATATACACAACAGACACA 199 26 0 38.46 58.508 12.00 1.00 7.492 220 CACGTGTATATACACAACAGACACAC 198 26 0 42.31 58.508 12.00 1.00 7.492 221 ACGTGTATATACACAACAGACACACA 197 26 0 38.46 58.508 12.00 0.00 7.492 222 CGTGTATATACACAACAGACACACAC 196 26 0 42.31 58.508 12.00 0.00 7.492 223 ATGTATATGCACAATACCACACATGTT 128 27 0 33.33 60.562 6.00 4.00 7.562 224 GATCTACACACACACACAACATACAC 247 26 0 42.31 58.431 4.00 0.00 7.569 225 ATATACACAACAGACACACACACACAA 191 27 0 37.04 60.577 4.00 0.00 7.577 226 TATATGCACAATACCACACATGTTTA 125 26 0 30.77 58.395 6.00 4.00 7.605 227 GATCTACACACACACACAACATACACT 247 27 0 40.74 59.376 4.00 1.00 7.624 228 ATCTACACACACACACAACATACACTC 246 27 0 40.74 59.376 2.00 0.00 7.624 229 CACACACACACAACATACACTCTAG 241 25 0 44.00 57.370 4.00 4.00 7.630 230 TGTATATACACAACAGACACACACACA 194 27 0 37.04 59.363 10.00 0.00 7.637 231 TATATACACAACAGACACACACACACA 192 27 0 37.04 59.363 6.00 0.00 7.637 232 CATCCATACACAACACACATGTATA 146 25 0 36.00 57.352 6.00 4.00 7.648 233 TCCATACACAACACACATGTATATG 144 25 0 36.00 57.352 6.00 6.00 7.648 234 CCATACACAACACACATGTATATGCA 143 26 0 38.46 61.654 6.00 6.00 7.654 235 CACACATGTATATGCACAATACCACA 133 26 0 38.46 61.654 6.00 2.00 7.654 236 CACATGTATATGCACAATACCACACA 131 26 0 38.46 61.654 6.00 0.00 7.654 237 ACACACGTGTATATACACAACAGACAC 201 27 0 40.74 59.293 12.00 3.00 7.707 238 ACACGTGTATATACACAACAGACACAC 199 27 0 40.74 59.293 12.00 1.00 7.707 239 ACGTGTATATACACAACAGACACACAC 197 27 0 40.74 59.293 12.00 0.00 7.707 240 CACACAGTCAAGAAGCTACATCCATA 164 26 0 42.31 61.740 4.00 2.00 7.740 241 CACAGTCAAGAAGCTACATCCATACA 162 26 0 42.31 61.740 4.00 0.00 7.740 242 CAGTCAAGAAGCTACATCCATACACA 160 26 0 42.31 61.740 4.00 0.00 7.740 243 CTACACACACACACAACATACACTCT 244 26 0 42.31 58.251 2.00 1.00 7.749 244 ACACACACACACAACATACACTCTAG 242 26 0 42.31 58.251 4.00 4.00 7.749 245 TCTACACACACACACAACATACACT 245 25 0 40.00 57.217 2.00 1.00 7.783 246 CTACACACACACACAACATACACTC 244 25 0 44.00 57.217 2.00 0.00 7.783 247 TACACACACACACAACATACACTCT 243 25 0 40.00 57.217 2.00 1.00 7.783 248 ACACACACACACAACATACACTCTA 242 25 0 40.00 57.217 3.00 3.00 7.783 249 TACATCCATACACAACACACATGTAT 148 26 0 34.62 58.200 6.00 6.00 7.800 250 ACATCCATACACAACACACATGTATA 147 26 0 34.62 58.200 6.00 4.00 7.800 251 CACAACACACAGTCAAGAAGCTACAT 169 26 0 42.31 61.844 4.00 4.00 7.844 252 GAACACACGTGTATATACACAACAGA 203 26 0 38.46 58.139 12.00 4.00 7.861 253 GTATATGCACAATACCACACATGTTTA 126 27 0 33.33 59.133 6.00 4.00 7.867 254 CTACATCCATACACAACACACATGTAT 149 27 0 37.04 59.113 6.00 6.00 7.887 255 ACACACACACAACACACAGTCAAGA 176 25 0 44.00 62.889 3.00 2.00 7.889 256 ACAACACACATGTATATGCACAATA 137 25 0 32.00 57.073 6.00 4.00 7.927 257 CAACACACATGTATATGCACAATAC 136 25 0 36.00 57.073 6.00 6.00 7.927 258 AGAACACACGTGTATATACACAACAGA 204 27 0 37.04 59.049 12.00 4.00 7.951 259 ATCCATACACAACACACATGTATATGC 145 27 0 37.04 61.010 6.00 4.00 8.010 260 ACAACACACATGTATATGCACAATACC 137 27 0 37.04 61.034 6.00 4.00 8.034 261 AACACACATGTATATGCACAATACCAC 135 27 0 37.04 61.034 6.00 2.00 8.034 262 CACACGTGTATATACACAACAGACACA 200 27 0 40.74 61.042 12.00 1.00 8.042 263 CACGTGTATATACACAACAGACACACA 198 27 0 40.74 61.042 12.00 0.00 8.042 264 CGTGTATATACACAACAGACACACACA 196 27 0 40.74 61.042 12.00 0.00 8.042 265 TCAAGAAGCTACATCCATACACAACA 157 26 0 38.46 62.056 4.00 0.00 8.056 266 GAACACACGTGTATATACACAACAGAC 203 27 0 40.74 58.926 12.00 2.00 8.074 267 ACAACACACATGTATATGCACAATAC 137 26 0 34.62 57.913 6.00 6.00 8.087 268 TATACACAACAGACACACACACACAAC 190 27 0 40.74 61.092 4.00 0.00 8.092 269 AGTCAAGAAGCTACATCCATACACAAC 159 27 0 40.74 61.126 4.00 0.00 8.126 270 GCTACATCCATACACAACACACATGT 150 26 0 42.31 62.163 6.00 6.00 8.163 271 GCACAATACCACACATGTTTAACAGA 120 26 0 38.46 62.185 7.00 3.00 8.185 272 AGCTACATCCATACACAACACACATG 151 26 0 42.31 62.266 4.00 4.00 8.266 273 CATCCATACACAACACACATGTATAT 146 26 0 34.62 57.730 6.00 6.00 8.270 274 ATCCATACACAACACACATGTATATG 145 26 0 34.62 57.730 6.00 6.00 8.270 275 ACACACAACACACAGTCAAGAAGCTA 172 26 0 42.31 62.367 4.00 2.00 8.367 276 CACACAACACACAGTCAAGAAGCTAC 171 26 0 46.15 62.367 4.00 4.00 8.367 277 ACACAACACACAGTCAAGAAGCTACA 170 26 0 42.31 62.367 4.00 2.00 8.367 278 GAAGCTACATCCATACACAACACACA 153 26 0 42.31 62.368 4.00 0.00 8.368 279 ATCTACACACACACACAACATACACT 246 26 0 38.46 57.620 2.00 1.00 8.380 280 ATGCACAATACCACACATGTTTAACA 122 26 0 34.62 62.397 7.00 7.00 8.397 281 TGTATATGCACAATACCACACATGTTT 127 27 0 33.33 61.445 6.00 6.00 8.445 282 ACACACAGTCAAGAAGCTACATCCAT 165 26 0 42.31 62.454 4.00 2.00 8.454 283 ACATCCATACACAACACACATGTATAT 147 27 0 33.33 58.526 6.00 6.00 8.474 284 ACACACAACATACACTCTAGACACAG 236 26 0 42.31 57.508 6.00 1.00 8.492 285 AGAACACACGTGTATATACACAACAG 204 26 0 38.46 57.375 12.00 6.00 8.625 286 ACACACACACAACATACACTCTAGAC 240 26 0 42.31 57.364 6.00 4.00 8.636 287 ACACACACAACATACACTCTAGACAC 238 26 0 42.31 57.364 6.00 0.00 8.636 288 TACACACACACACAACATACACTCTA 243 26 0 38.46 57.330 3.00 3.00 8.670 289 CTACACACACACACAACATACACTCTA 244 27 0 40.74 58.323 3.00 3.00 8.677 290 TACACACACACACAACATACACTCTAG 243 27 0 40.74 58.323 4.00 4.00 8.677 291 CATACACAACACACATGTATATGCACA 142 27 0 37.04 61.688 6.00 4.00 8.688 292 TACATCCATACACAACACACATGTATA 148 27 0 33.33 58.271 6.00 4.00 8.729 293 AACACACGTGTATATACACAACAGAC 202 26 0 38.46 57.237 12.00 2.00 8.763 294 AAGAAGCTACATCCATACACAACACAC 155 27 0 40.74 61.811 4.00 0.00 8.811 295 CACATGTATATGCACAATACCACACAT 131 27 0 37.04 61.814 6.00 2.00 8.814 296 ACATGTATATGCACAATACCACACATG 130 27 0 37.04 61.814 6.00 4.00 8.814 297 CATGTATATGCACAATACCACACATGT 129 27 0 37.04 61.814 6.00 6.00 8.814 298 CACAATACCACACATGTTTAACAGACA 119 27 0 37.04 62.089 7.00 3.00 9.089 299 GCTACATCCATACACAACACACATGTA 150 27 0 40.74 62.100 6.00 4.00 9.100 300 CCATACACAACACACATGTATATGCAC 143 27 0 40.74 62.312 6.00 4.00 9.312 301 ACACACATGTATATGCACAATACCACA 134 27 0 37.04 62.312 6.00 2.00 9.312 302 CACACATGTATATGCACAATACCACAC 133 27 0 40.74 62.312 6.00 0.00 9.312 303 ACACATGTATATGCACAATACCACACA 132 27 0 37.04 62.312 6.00 0.00 9.312 304 TATGCACAATACCACACATGTTTAACA 123 27 0 33.33 62.329 7.00 7.00 9.329 305 ACACACAGTCAAGAAGCTACATCCATA 165 27 0 40.74 62.383 4.00 2.00 9.383 306 CACACAGTCAAGAAGCTACATCCATAC 164 27 0 44.44 62.383 4.00 0.00 9.383 307 ACACAGTCAAGAAGCTACATCCATACA 163 27 0 40.74 62.383 4.00 0.00 9.383 308 CACAGTCAAGAAGCTACATCCATACAC 162 27 0 44.44 62.383 4.00 0.00 9.383 309 ACAGTCAAGAAGCTACATCCATACACA 161 27 0 40.74 62.383 4.00 0.00 9.383 310 GTGTATATACACAACAGACACACACAC 195 27 0 40.74 57.575 12.00 0.00 9.425 311 GTATATACACAACAGACACACACACAC 193 27 0 40.74 57.575 8.00 0.00 9.425 312 GAAGCTACATCCATACACAACACACAT 153 27 0 40.74 62.494 4.00 2.00 9.494 313 ACACAACACACAGTCAAGAAGCTACAT 170 27 0 40.74 62.495 4.00 4.00 9.495 314 TAGAACACACGTGTATATACACAACAG 205 27 0 37.04 57.472 12.00 6.00 9.528 315 GTCAAGAAGCTACATCCATACACAACA 158 27 0 40.74 62.685 4.00 0.00 9.685 316 TCAAGAAGCTACATCCATACACAACAC 157 27 0 40.74 62.685 4.00 0.00 9.685 317 CAACACACATGTATATGCACAATACCA 136 27 0 37.04 62.716 6.00 4.00 9.716 318 CAGTCAAGAAGCTACATCCATACACAA 160 27 0 40.74 62.778 4.00 0.00 9.778 319 GCACAATACCACACATGTTTAACAGAC 120 27 0 40.74 62.806 7.00 1.00 9.806 320 AGCTACATCCATACACAACACACATGT 151 27 0 40.74 62.902 6.00 6.00 9.902 primer3-2.3.6/test/primer_list_last/targets_test.rev0000664000077300007730000000041112231156244021632 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 CAGTGTGTACATGGCGGC 160 18 0 61.11 59.685 6.00 3.00 2.315 primer3-2.3.6/test/primer_list_last/long_seq.rev0000664000077300007730000021725112231156244020745 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 agctagctagcatcgatcgc 391 20 0 55.00 59.880 10.00 6.00 0.120 1 cccagctagctagcatcgtc 676 20 0 60.00 60.140 10.00 4.00 0.140 2 catcgatcgatgtagcatcg 335 20 0 50.00 60.205 12.00 8.00 0.205 3 gtagcatgcatgcatcgatc 464 20 0 50.00 60.222 10.00 6.00 0.222 4 agcatcgcctagctagcatc 427 20 0 55.00 59.742 8.00 4.00 0.258 5 gtcagctagctagatcgccg 600 20 0 60.00 60.278 8.00 6.00 0.278 6 ctagctagatcgccgctacg 595 20 0 60.00 60.285 8.00 2.00 0.285 7 cgatgcgctagctagtagcc 694 20 0 60.00 60.293 8.00 6.00 0.293 8 gctagctagcatcgcctagc 434 20 0 60.00 59.528 10.00 6.00 0.472 9 agctagctagatcgccgcta 597 20 0 55.00 59.523 8.00 4.00 0.477 10 ctagctagccgcacatcgat 104 20 0 55.00 60.531 8.00 6.00 0.531 11 gctagctagatcgccgctac 596 20 0 60.00 59.393 8.00 2.00 0.607 12 tcgcctagctagcatcgatc 423 20 0 55.00 60.615 8.00 4.00 0.615 13 gcctagctagcatcgatcga 421 20 0 55.00 60.615 8.00 8.00 0.615 14 tcgcgtatcagtcagctagc 486 20 0 55.00 59.351 6.00 6.00 0.649 15 ccagctagctagcatcgtca 675 20 0 55.00 59.347 10.00 1.00 0.653 16 agtagcatgcatgcatcgat 465 20 0 45.00 59.285 10.00 8.00 0.715 17 gatgcgctagctagtagccc 693 20 0 60.00 59.256 8.00 8.00 0.744 18 gcatcgatcgatgtagcatc 336 20 0 50.00 59.213 12.00 6.00 0.787 19 tgcgctagctagtagcccag 691 20 0 60.00 60.821 10.00 6.00 0.821 20 atcgcctagctagcatcgat 424 20 0 50.00 59.074 8.00 6.00 0.926 21 cgctagctagtagcccagct 689 20 0 60.00 59.068 8.00 5.00 0.932 22 tagtagcatgcatgcatcga 466 20 0 45.00 58.989 10.00 8.00 1.011 23 ctagtagcatgcatgcatcga 467 21 0 47.62 60.014 10.00 8.00 1.014 24 gcatcgatcgatcagctagc 412 20 0 55.00 61.025 10.00 6.00 1.025 25 gctagcatcgatcgatcagtc 135 21 0 52.38 59.961 10.00 6.00 1.039 26 agcatcgatcgatcagtcag 132 20 0 50.00 58.936 10.00 2.00 1.064 27 catcgatcgatcagtcagct 130 20 0 50.00 58.936 10.00 4.00 1.064 28 tagctagctagcatcgatcgc 392 21 0 52.38 59.894 10.00 6.00 1.106 29 gctagctagcatcgatgatcg 567 21 0 52.38 60.117 10.00 8.00 1.117 30 tagctagatcgccgctacga 594 20 0 55.00 61.150 6.00 4.00 1.150 31 ctagctagcatcgatcgcg 389 19 0 57.89 59.848 8.00 4.00 1.152 32 cgcgcagagatgatagtagc 374 20 0 55.00 58.801 4.00 3.00 1.199 33 cgctacgatcgatcagctag 582 20 0 55.00 58.799 8.00 8.00 1.201 34 cgcgcagagatgatagtagct 374 21 0 52.38 59.789 4.00 4.00 1.211 35 agcatcgatcgatgtagcatc 337 21 0 47.62 60.222 12.00 6.00 1.222 36 tagcatcgcctagctagcatc 428 21 0 52.38 59.761 8.00 4.00 1.239 37 cgatcgatcagctagtcagct 614 21 0 52.38 59.755 8.00 5.00 1.245 38 cgatcgatcagtcagctagct 127 21 0 52.38 59.755 8.00 8.00 1.245 39 ctagctagccgcacatcga 104 19 0 57.89 60.272 8.00 4.00 1.272 40 cgatcgatcagctagtcagc 614 20 0 55.00 58.726 8.00 3.00 1.274 41 cgatcgatcagtcagctagc 127 20 0 55.00 58.726 8.00 6.00 1.274 42 catcgcctagctagcatcg 425 19 0 57.89 59.703 8.00 4.00 1.297 43 agctagctagatcgccgctac 597 21 0 57.14 60.297 8.00 2.00 1.297 44 ccagctagctagcatcgtcag 675 21 0 57.14 60.319 10.00 2.00 1.319 45 tcgcgtatcagtcagctagct 486 21 0 52.38 60.328 8.00 8.00 1.328 46 agctagcatcgcgtatcagtc 494 21 0 52.38 59.657 6.00 1.00 1.343 47 atcgcgtatcagtcagctagc 487 21 0 52.38 59.657 6.00 6.00 1.343 48 gctagcatcgcgtatcagtc 493 20 0 55.00 58.655 6.00 1.00 1.345 49 acgatcgatcagctagtcagc 615 21 0 52.38 59.618 8.00 3.00 1.382 50 agctagatcgccgctacgat 593 20 0 55.00 61.387 6.00 6.00 1.387 51 ctagcatcgcctagctagca 429 20 0 55.00 58.609 8.00 4.00 1.391 52 ccgctacgatcgatcagcta 583 20 0 55.00 61.420 8.00 6.00 1.420 53 agctagctagcatcgcctagc 435 21 0 57.14 60.424 10.00 6.00 1.424 54 gctagctagcatcgcctagct 434 21 0 57.14 60.424 10.00 8.00 1.424 55 tagctagccgcacatcgatc 103 20 0 55.00 61.432 6.00 4.00 1.432 56 tagctagccgcacatcgat 103 19 0 52.63 59.563 6.00 6.00 1.437 57 cagctagctagcatcgtcagc 674 21 0 57.14 60.464 10.00 2.00 1.464 58 gctagatcgccgctacgat 592 19 0 57.89 60.493 6.00 6.00 1.493 59 agctagctagatcgccgct 597 19 0 57.89 59.495 8.00 3.00 1.505 60 agcatcgcgtatcagtcagc 490 20 0 55.00 61.520 4.00 2.00 1.520 61 gcatcgcgtatcagtcagct 489 20 0 55.00 61.520 4.00 4.00 1.520 62 tagctagctagccgcacatc 107 20 0 55.00 58.466 10.00 0.00 1.534 63 cgcgtatcagtcagctagct 485 20 0 55.00 58.460 8.00 8.00 1.540 64 gcatcgcgtatcagtcagc 489 19 0 57.89 60.553 4.00 2.00 1.553 65 catcgcctagctagcatcga 425 20 0 55.00 61.554 8.00 4.00 1.554 66 ctagctagctagccgcacatc 108 21 0 57.14 59.442 12.00 0.00 1.558 67 tcagctagctagatcgccg 599 19 0 57.89 59.435 8.00 6.00 1.565 68 agcatcgatgatgatcagct 533 20 0 45.00 58.361 8.00 6.00 1.639 69 tagctagatcgccgctacg 594 19 0 57.89 59.339 6.00 2.00 1.661 70 tcgatcgatcagtcagctagc 128 21 0 52.38 60.661 8.00 6.00 1.661 71 tcgcgcagagatgatagtagc 375 21 0 52.38 60.662 4.00 3.00 1.662 72 gctagctagcatcgcgtatca 497 21 0 52.38 60.662 10.00 1.00 1.662 73 gcgctagctagtagcccagc 690 20 0 65.00 61.663 8.00 7.00 1.663 74 tagtagcatgcatgcatcgat 466 21 0 42.86 59.324 10.00 8.00 1.676 75 tagcatgcatgcatcgatc 463 19 0 47.37 59.309 10.00 7.00 1.691 76 agcatcgatcgatgtagcat 337 20 0 45.00 58.299 12.00 4.00 1.701 77 agcatcgatgatcagctagct 209 21 0 47.62 59.216 8.00 8.00 1.784 78 cgatcgatcagctagctagca 577 21 0 52.38 60.787 10.00 8.00 1.787 79 cgatcagctagctagcatcga 573 21 0 52.38 60.787 10.00 8.00 1.787 80 agctagcatcgatcgatcag 417 20 0 50.00 58.173 10.00 8.00 1.827 81 ctagcatcgatcgatcagct 415 20 0 50.00 58.173 10.00 8.00 1.827 82 agcatcgatcgatcagctag 413 20 0 50.00 58.173 10.00 6.00 1.827 83 catcgatcgatcagctagct 411 20 0 50.00 58.173 10.00 8.00 1.827 84 agctagcatcgatcgatcag 136 20 0 50.00 58.173 10.00 8.00 1.827 85 agcatcgatgatcagctagc 209 20 0 50.00 58.173 8.00 6.00 1.827 86 gcatcgatgatcagctagct 208 20 0 50.00 58.173 8.00 8.00 1.827 87 atcgcctagctagcatcgatc 424 21 0 52.38 60.845 8.00 4.00 1.845 88 cgctagctagtagcccagcta 689 21 0 57.14 59.110 8.00 6.00 1.890 89 gcatcgatcgatcagtcagc 131 20 0 55.00 61.922 10.00 3.00 1.922 90 ctagtagcatgcatgcatcg 467 20 0 50.00 58.074 10.00 8.00 1.926 91 agctagcatcgatcgatcagt 136 21 0 47.62 59.073 10.00 6.00 1.927 92 gcccagctagctagcatcgt 677 20 0 60.00 61.940 10.00 6.00 1.940 93 cctagctagcatcgatcgatc 420 21 0 52.38 59.060 10.00 8.00 1.940 94 cgatcgcgcagagatgatag 378 20 0 55.00 61.980 6.00 1.00 1.980 95 gatcgcgcagagatgatagt 377 20 0 50.00 58.018 4.00 1.00 1.982 96 gctagcatcgatcgatcagt 135 20 0 50.00 58.018 10.00 6.00 1.982 97 ctagcatcgatcgatcagtcag 134 22 0 50.00 60.000 10.00 6.00 2.000 98 catcgatcgatcagtcagctag 130 22 0 50.00 60.000 10.00 4.00 2.000 99 cgctagctagtagcccagctag 689 22 0 59.09 59.992 8.00 8.00 2.008 100 ctagcatcgatcgatcagtca 134 21 0 47.62 58.990 10.00 5.00 2.010 101 tagcatcgatcgatcagtcag 133 21 0 47.62 58.990 10.00 2.00 2.010 102 catcgatcgatcagtcagcta 130 21 0 47.62 58.990 10.00 2.00 2.010 103 tagcatcgatcgatgtagca 338 20 0 45.00 57.987 12.00 7.00 2.013 104 atgcgctagctagtagcccag 692 21 0 57.14 61.035 10.00 8.00 2.035 105 cgatcgcgcagagatgata 378 19 0 52.63 61.047 6.00 2.00 2.047 106 tagcatcgcctagctagcat 428 20 0 50.00 57.952 8.00 6.00 2.048 107 gctagctagcatcgcgtatc 497 20 0 55.00 57.951 10.00 2.00 2.049 108 agcccagctagctagcatcg 678 20 0 60.00 62.053 10.00 8.00 2.053 109 agctagctagcatcgcgtatc 498 21 0 52.38 58.940 10.00 2.00 2.060 110 ctagcatcgcctagctagcat 429 21 0 52.38 58.937 8.00 6.00 2.063 111 tcagctagctagcatcgatga 570 21 0 47.62 58.934 10.00 8.00 2.066 112 agtagcatgcatgcatcga 465 19 0 47.37 58.933 10.00 8.00 2.067 113 cgatgtagcatcgatcagtagc 328 22 0 50.00 59.895 8.00 2.00 2.105 114 gctagctagcatcgatcgc 390 19 0 57.89 58.889 10.00 6.00 2.111 115 agctagctacgatcgatcagc 623 21 0 52.38 58.876 8.00 6.00 2.124 116 gctagctacgatcgatcagct 622 21 0 52.38 58.876 8.00 8.00 2.124 117 gctacgatcgatcagctagct 581 21 0 52.38 58.876 10.00 8.00 2.124 118 tagcatcgatcgatcagtca 133 20 0 45.00 57.875 10.00 4.00 2.125 119 gctagctacgatcgatcagc 622 20 0 55.00 57.846 8.00 6.00 2.154 120 agtcagctagctagatcgccg 601 21 0 57.14 61.177 8.00 6.00 2.177 121 cagctagctagatcgccgct 598 20 0 60.00 62.179 8.00 3.00 2.179 122 gcccagctagctagcatcg 677 19 0 63.16 61.184 10.00 8.00 2.184 123 agctagatcgccgctacga 593 19 0 57.89 61.184 4.00 4.00 2.184 124 agctagctacgatcgatcagct 623 22 0 50.00 59.816 8.00 8.00 2.184 125 agtagcatgcatgcatcgatc 465 21 0 47.62 61.188 10.00 6.00 2.188 126 cgcgcagagatgatagtagcta 374 22 0 50.00 59.806 6.00 6.00 2.194 127 agctagcatcgcgtatcagt 494 20 0 50.00 57.781 6.00 1.00 2.219 128 tgcatcgatcgatgatagtagc 454 22 0 45.45 60.225 12.00 4.00 2.225 129 tagcatcgatcgatgtagcatc 338 22 0 45.45 60.225 12.00 6.00 2.225 130 cgatcgatcagctagtcagcta 614 22 0 50.00 59.775 8.00 3.00 2.225 131 cgatcgatcagtcagctagcta 127 22 0 50.00 59.775 8.00 6.00 2.225 132 gctagatcgccgctacga 592 18 0 61.11 60.228 4.00 4.00 2.228 133 ctagctagctagcatcgcgt 500 20 0 55.00 57.771 12.00 4.00 2.229 134 catgatgatcgatcagtcagct 284 22 0 45.45 60.249 12.00 7.00 2.249 135 gcatcgcctagctagcatc 426 19 0 57.89 58.740 8.00 4.00 2.260 136 atcgcctagctagcatcga 424 19 0 52.63 58.738 8.00 5.00 2.262 137 tcgcctagctagcatcgat 423 19 0 52.63 58.738 8.00 6.00 2.262 138 cgcctagctagcatcgatc 422 19 0 57.89 58.738 8.00 4.00 2.262 139 gcctagctagcatcgatcg 421 19 0 57.89 58.738 8.00 6.00 2.262 140 gctagatcgccgctacgatc 592 20 0 60.00 62.271 8.00 8.00 2.271 141 ctagtagcatgcatgcatcgat 467 22 0 45.45 60.280 10.00 8.00 2.280 142 ctagcatcgcgtatcagtcag 492 21 0 52.38 58.715 4.00 1.00 2.285 143 catcgcgtatcagtcagctag 488 21 0 52.38 58.715 4.00 4.00 2.285 144 agctagctagcatcgtcagct 673 21 0 52.38 58.692 10.00 4.00 2.308 145 cagctagctagatcgccgc 598 19 0 63.16 61.320 8.00 3.00 2.320 146 tagctagcatcgcgtatcagtc 495 22 0 50.00 59.680 6.00 1.00 2.320 147 tcgcgtatcagtcagctagcta 486 22 0 50.00 60.326 8.00 6.00 2.326 148 agctagctagcatcgtcagc 673 20 0 55.00 57.659 10.00 2.00 2.341 149 gctagctagcatcgtcagct 672 20 0 55.00 57.659 10.00 4.00 2.341 150 agctagcatcgtcagctagc 669 20 0 55.00 57.659 7.00 7.00 2.341 151 gctagcatcgtcagctagct 668 20 0 55.00 57.659 8.00 8.00 2.341 152 tagctagatcgccgctacgat 594 21 0 52.38 61.348 6.00 6.00 2.348 153 gctacgatcgatcagctagtca 618 22 0 50.00 59.643 8.00 2.00 2.357 154 tacgatcgatcagctagtcagc 616 22 0 50.00 59.643 8.00 3.00 2.357 155 ctagcatcgcgtatcagtca 492 20 0 50.00 57.632 4.00 1.00 2.368 156 tagcatcgcgtatcagtcag 491 20 0 50.00 57.632 4.00 1.00 2.368 157 catcgcgtatcagtcagcta 488 20 0 50.00 57.632 4.00 3.00 2.368 158 ctagctagctagccgcacat 108 20 0 55.00 57.626 12.00 2.00 2.374 159 ctagatcgccgctacgatc 591 19 0 57.89 58.588 8.00 8.00 2.412 160 tagctagctagcatcgcctagc 436 22 0 54.55 60.419 10.00 6.00 2.419 161 catgcatcgatcgatgatagt 456 21 0 42.86 58.563 12.00 8.00 2.437 162 gctagctagatcgccgcta 596 19 0 57.89 58.547 8.00 4.00 2.453 163 tagcccagctagctagcatc 679 20 0 55.00 57.541 10.00 6.00 2.459 164 ccgctacgatcgatcagct 583 19 0 57.89 61.465 8.00 4.00 2.465 165 agctagccgcacatcgat 102 18 0 55.56 59.533 6.00 6.00 2.467 166 cgcgtatcagtcagctagcta 485 21 0 52.38 58.528 8.00 6.00 2.472 167 gctagcatcgcgtatcagtca 493 21 0 52.38 61.473 6.00 1.00 2.473 168 tagcatcgcgtatcagtcagc 491 21 0 52.38 61.473 4.00 2.00 2.473 169 gcatcgcgtatcagtcagcta 489 21 0 52.38 61.473 4.00 3.00 2.473 170 agctagccgcacatcgatc 102 19 0 57.89 61.479 6.00 4.00 2.479 171 gccgctacgatcgatcag 584 18 0 61.11 60.490 8.00 6.00 2.490 172 ccgctacgatcgatcagc 583 18 0 61.11 60.490 8.00 6.00 2.490 173 gctagccgcacatcgatc 101 18 0 61.11 60.500 6.00 4.00 2.500 174 cgcgtatcagtcagctagctag 485 22 0 54.55 59.491 8.00 8.00 2.509 175 agctagcatcgatgatgatcag 537 22 0 45.45 59.462 8.00 6.00 2.538 176 ctagcatcgatgatgatcagct 535 22 0 45.45 59.462 8.00 6.00 2.538 177 agcatcgatgatgatcagctag 533 22 0 45.45 59.462 8.00 5.00 2.538 178 catcgatgatgatcagctagct 531 22 0 45.45 59.462 8.00 8.00 2.538 179 agctagcatgatgatcgatcag 290 22 0 45.45 59.462 10.00 8.00 2.538 180 agctagcatcgatgatgatca 537 21 0 42.86 58.438 8.00 7.00 2.562 181 gctagcatcgatgatgatcag 536 21 0 47.62 58.438 8.00 6.00 2.562 182 ctagcatcgatgatgatcagc 535 21 0 47.62 58.438 8.00 7.00 2.562 183 tagcatcgatgatgatcagct 534 21 0 42.86 58.438 8.00 6.00 2.562 184 agcatcgatgatgatcagcta 533 21 0 42.86 58.438 8.00 7.00 2.562 185 gcatcgatgatgatcagctag 532 21 0 47.62 58.438 8.00 5.00 2.562 186 catcgatgatgatcagctagc 531 21 0 47.62 58.438 8.00 6.00 2.562 187 gctagcatgatgatcgatcag 289 21 0 47.62 58.438 10.00 8.00 2.562 188 acgatcgatcagctagtcagct 615 22 0 50.00 60.566 8.00 5.00 2.566 189 atcgcgtatcagtcagctagct 487 22 0 50.00 60.570 8.00 8.00 2.570 190 gtagcccagctagctagcatc 680 21 0 57.14 58.416 10.00 6.00 2.584 191 agctagctagccgcacatc 106 19 0 57.89 58.394 10.00 0.00 2.606 192 tagcatcgatcgatgtagcat 338 21 0 42.86 58.376 12.00 4.00 2.624 193 ctagcatcgcctagctagcatc 429 22 0 54.55 60.642 8.00 4.00 2.642 194 agtagcccagctagctagcatc 681 22 0 54.55 59.355 10.00 6.00 2.645 195 gctagcatgatgatcgatcagt 289 22 0 45.45 59.325 10.00 8.00 2.675 196 gctagcatcgatgatgatca 536 20 0 45.00 57.307 8.00 7.00 2.693 197 tagcatcgatgatgatcagc 534 20 0 45.00 57.307 8.00 7.00 2.693 198 gcatcgatgatgatcagcta 532 20 0 45.00 57.307 8.00 7.00 2.693 199 agctagatcgccgctacg 593 18 0 61.11 59.301 4.00 2.00 2.699 200 gctagctagtagcatgcatgca 472 22 0 50.00 60.706 10.00 8.00 2.706 201 agcatgcatgcatcgatc 462 18 0 50.00 59.265 10.00 4.00 2.735 202 catcgcctagctagcatcgat 425 21 0 52.38 61.735 8.00 6.00 2.735 203 gatcagctagctagcatcgatg 572 22 0 50.00 59.256 10.00 8.00 2.744 204 atcagctagctagcatcgatga 571 22 0 45.45 59.256 10.00 8.00 2.744 205 tcagctagctagcatcgatgat 570 22 0 45.45 59.256 10.00 8.00 2.744 206 cagctagctagcatcgatgatc 569 22 0 50.00 59.256 10.00 6.00 2.744 207 tagcatcgatgatcagctagct 210 22 0 45.45 59.256 8.00 8.00 2.744 208 agcatcgatgatcagctagcta 209 22 0 45.45 59.256 8.00 6.00 2.744 209 gcatcgatgatcagctagctag 208 22 0 50.00 59.256 8.00 8.00 2.744 210 tagcatcgatgatcagctagc 210 21 0 47.62 58.255 8.00 6.00 2.745 211 gcatcgatgatcagctagcta 208 21 0 47.62 58.255 8.00 6.00 2.745 212 ctagctagcatcgatcgatca 419 21 0 47.62 58.254 10.00 6.00 2.746 213 tagctagcatcgatcgatcag 418 21 0 47.62 58.254 10.00 8.00 2.746 214 tagcatcgatcgatcagctag 414 21 0 47.62 58.254 10.00 7.00 2.746 215 catcgatcgatcagctagcta 411 21 0 47.62 58.254 10.00 6.00 2.746 216 ctagctagcatcgatcgatca 138 21 0 47.62 58.254 10.00 6.00 2.746 217 tagctagcatcgatcgatcag 137 21 0 47.62 58.254 10.00 8.00 2.746 218 ctagctagcatcgatcgatcag 419 22 0 50.00 59.252 10.00 8.00 2.748 219 catcgatcgatcagctagctag 411 22 0 50.00 59.252 10.00 8.00 2.748 220 ctagctagcatcgatcgatcag 138 22 0 50.00 59.252 10.00 8.00 2.748 221 cccagctagctagcatcgt 676 19 0 57.89 58.242 10.00 6.00 2.758 222 ctagctagctagcatcgatcgc 393 22 0 54.55 60.766 12.00 6.00 2.766 223 tagctagccgcacatcga 103 18 0 55.56 59.232 6.00 4.00 2.768 224 agtagcccagctagctagca 681 20 0 55.00 57.212 10.00 8.00 2.788 225 agctagtagcatgcatgcatc 469 21 0 47.62 58.187 10.00 8.00 2.813 226 gtagcatgcatgcatcgat 464 19 0 47.37 58.187 10.00 8.00 2.813 227 gctagcatactagcatcgatcg 348 22 0 50.00 59.173 10.00 6.00 2.827 228 tagctagcatcgatcgatca 418 20 0 45.00 57.158 10.00 6.00 2.842 229 tagctagcatcgatcgatca 137 20 0 45.00 57.158 10.00 6.00 2.842 230 gctagtagcatgcatgcatcg 468 21 0 52.38 61.851 10.00 8.00 2.851 231 agctagctagcatcgcgtat 498 20 0 50.00 57.129 10.00 4.00 2.871 232 gctagtagcatcgatgatcagc 215 22 0 50.00 59.121 8.00 8.00 2.879 233 gtagcatcgatgatcagctagc 211 22 0 50.00 59.121 8.00 6.00 2.879 234 gatcgcgcagagatgatagtag 377 22 0 50.00 59.118 4.00 1.00 2.882 235 actagctagcatcgatcgatca 139 22 0 45.45 59.118 10.00 6.00 2.882 236 tagctagcatcgatcgatcagt 137 22 0 45.45 59.118 10.00 6.00 2.882 237 atcgcgcagagatgatagtagc 376 22 0 50.00 60.887 4.00 3.00 2.887 238 gatcgcgcagagatgatagta 377 21 0 47.62 58.106 4.00 3.00 2.894 239 agctagcatcgatcgatcagtc 136 22 0 50.00 60.895 10.00 6.00 2.895 240 atcgatcgatcagtcagctagc 129 22 0 50.00 60.895 10.00 6.00 2.895 241 gctagtagcatgcatgcatc 468 20 0 50.00 57.086 10.00 8.00 2.914 242 gctagcatcgatcgatcagct 416 21 0 52.38 61.918 10.00 8.00 2.918 243 agcatcgatcgatcagctagc 413 21 0 52.38 61.918 10.00 7.00 2.918 244 gcatcgatcgatcagctagct 412 21 0 52.38 61.918 10.00 8.00 2.918 245 gcgctagctagtagcccag 690 19 0 63.16 58.067 8.00 5.00 2.933 246 cgctagctagtagcccagc 689 19 0 63.16 58.067 8.00 7.00 2.933 247 gatgatcgatcagtcagctagc 281 22 0 50.00 59.062 10.00 6.00 2.938 248 tcgatcgcgcagagatgata 379 20 0 50.00 62.940 6.00 2.00 2.940 249 agctagctagccgcacatcg 106 20 0 60.00 62.979 10.00 2.00 2.979 250 ctagctagcatcgcgtatcag 496 21 0 52.38 58.020 8.00 2.00 2.980 251 tagcccagctagctagcatcg 679 21 0 57.14 61.990 10.00 8.00 2.990 252 ctagctagatcgccgctacga 595 21 0 57.14 61.990 8.00 4.00 2.990 253 cgatcgatcagctagctagcat 577 22 0 50.00 61.005 10.00 8.00 3.005 254 agctagctagcatcgatgatcg 568 22 0 50.00 61.005 10.00 8.00 3.005 255 tagctagctagcatcgcgtatc 499 22 0 50.00 58.987 10.00 2.00 3.013 256 gatgatcgatcagtcagctagct 281 23 0 47.83 60.025 10.00 8.00 3.025 257 actagctagcatcgatcgatcag 139 23 0 47.83 60.041 10.00 8.00 3.041 258 ctagctagcatcgatcgatcagt 138 23 0 47.83 60.041 10.00 6.00 3.041 259 agctagtagcatcgatgatcagc 216 23 0 47.83 60.048 8.00 8.00 3.048 260 gctagtagcatcgatgatcagct 215 23 0 47.83 60.048 8.00 7.00 3.048 261 agtagcatcgatgatcagctagc 212 23 0 47.83 60.048 8.00 6.00 3.048 262 gtagcatcgatgatcagctagct 211 23 0 47.83 60.048 8.00 8.00 3.048 263 agctagcatactagcatcgatcg 349 23 0 47.83 60.063 10.00 6.00 3.063 264 tagctagctacgatcgatcagc 624 22 0 50.00 58.927 10.00 6.00 3.073 265 gctacgatcgatcagctagcta 581 22 0 50.00 58.927 10.00 6.00 3.073 266 gctactagctagcatcgatcga 142 22 0 50.00 58.927 8.00 8.00 3.073 267 catcgatcgcgcagagat 381 18 0 55.56 61.080 6.00 4.00 3.080 268 atcgatcgcgcagagatg 380 18 0 55.56 61.080 6.00 2.00 3.080 269 cgatcgcgcagagatgat 378 18 0 55.56 61.080 6.00 3.00 3.080 270 agcatcgatcgatgtagca 337 19 0 47.37 57.890 12.00 3.00 3.110 271 cagctagctagatcgccgcta 598 21 0 57.14 62.111 8.00 4.00 3.111 272 tagctagcatcgcgtatcagt 495 21 0 47.62 57.875 6.00 1.00 3.125 273 gctagcatcgcctagctagca 430 21 0 57.14 62.125 9.00 5.00 3.125 274 ctagctagctagcatcgcgta 500 21 0 52.38 57.862 12.00 6.00 3.138 275 agcatcgcctagctagcat 427 19 0 52.63 57.861 8.00 6.00 3.139 276 ctagctagcatcgcgtatcagt 496 22 0 50.00 58.860 8.00 1.00 3.140 277 ctagctagctagcatcgcgtatc 500 23 0 52.17 59.860 12.00 2.00 3.140 278 tcagctagctagtagcatgcatg 475 23 0 47.83 59.856 8.00 8.00 3.144 279 tagtcagctagctagatcgccg 602 22 0 54.55 61.145 8.00 6.00 3.145 280 gctagctagccgcacatcg 105 19 0 63.16 62.150 10.00 2.00 3.150 281 tagtagcatgcatgcatcgatc 466 22 0 45.45 61.154 10.00 6.00 3.154 282 gcgcagagatgatagtagctagc 373 23 0 52.17 59.838 6.00 6.00 3.162 283 ctagctagctacgatcgatcagc 625 23 0 52.17 59.832 10.00 6.00 3.168 284 tagctagctacgatcgatcagct 624 23 0 47.83 59.832 10.00 8.00 3.168 285 gctacgatcgatcagctagctag 581 23 0 52.17 59.832 10.00 8.00 3.168 286 agctactagctagcatcgatcga 143 23 0 47.83 59.832 9.00 8.00 3.168 287 agcatcgatgatcagctagctag 209 23 0 47.83 60.170 8.00 8.00 3.170 288 tgatcgatcagtcagctagct 279 21 0 47.62 57.809 10.00 8.00 3.191 289 gatcgatcagctagtcagctagc 613 23 0 52.17 59.808 8.00 7.00 3.192 290 tagctagcatcgatcgcg 388 18 0 55.56 58.807 6.00 4.00 3.193 291 agcatcgatcgatcagtca 132 19 0 47.37 57.768 10.00 2.00 3.232 292 gcatcgatcgatcagtcag 131 19 0 52.63 57.768 10.00 2.00 3.232 293 catcgatcgatcagtcagc 130 19 0 52.63 57.768 10.00 2.00 3.232 294 gctagctagcatcgtcagcta 672 21 0 52.38 57.757 10.00 6.00 3.243 295 tagctagcatcgtcagctagc 670 21 0 52.38 57.757 7.00 7.00 3.243 296 gctagcatcgtcagctagcta 668 21 0 52.38 57.757 8.00 6.00 3.243 297 agctagctagcatcgcctagct 435 22 0 54.55 61.245 10.00 8.00 3.245 298 agtagctagctagcatcgcct 438 21 0 52.38 57.752 10.00 4.00 3.248 299 catgatgatcgatcagtcagcta 284 23 0 43.48 60.250 12.00 5.00 3.250 300 agctagctagcatcgtcagcta 673 22 0 50.00 58.749 10.00 6.00 3.251 301 gctagctagcatcgtcagctag 672 22 0 54.55 58.749 10.00 8.00 3.251 302 ctagctagcatcgtcagctagc 671 22 0 54.55 58.749 8.00 7.00 3.251 303 agctagcatcgtcagctagcta 669 22 0 50.00 58.749 9.00 6.00 3.251 304 gctagcatcgtcagctagctag 668 22 0 54.55 58.749 8.00 8.00 3.251 305 cgcgtatcagtcagctagctagt 485 23 0 52.17 60.254 8.00 6.00 3.254 306 agctagcatgatgatcgatcagt 290 23 0 43.48 60.271 10.00 8.00 3.271 307 ccgctacgatcgatcagctag 583 21 0 57.14 62.273 8.00 8.00 3.273 308 cgctacgatcgatcagcta 582 19 0 52.63 57.716 8.00 6.00 3.284 309 ctagctagccgcacatcgatc 104 21 0 57.14 62.288 8.00 4.00 3.288 310 tcgactagctagctacgatcgat 629 23 0 47.83 59.706 12.00 8.00 3.294 311 cgactagctagctacgatcgatc 628 23 0 52.17 59.706 10.00 8.00 3.294 312 agctagctagcatcgtcagctag 673 23 0 52.17 59.657 10.00 8.00 3.343 313 agctagcatcgtcagctagctag 669 23 0 52.17 59.657 9.00 8.00 3.343 314 cagctagctagcatcgtcagct 674 22 0 54.55 61.343 10.00 4.00 3.343 315 tagatcgccgctacgatcg 590 19 0 57.89 62.347 8.00 6.00 3.347 316 tcgatcagctagtcagctagc 611 21 0 52.38 57.646 7.00 7.00 3.354 317 catgcatcgatcgatgatagta 456 22 0 40.91 58.629 12.00 7.00 3.371 318 cgactagctagctacgatcga 628 21 0 52.38 57.613 10.00 6.00 3.387 319 agtagcccagctagctagcat 681 21 0 52.38 57.612 10.00 8.00 3.388 320 catgcatcgatcgatgatagtag 456 23 0 43.48 59.605 12.00 7.00 3.395 321 agcatcgcgtatcagtcagct 490 21 0 52.38 62.397 4.00 4.00 3.397 322 catgcatgcatcgatcga 460 18 0 50.00 61.413 10.00 8.00 3.413 323 tagcatcgcctagctagca 428 19 0 52.63 57.552 8.00 4.00 3.448 324 gcgctagctagtagcccagct 690 21 0 61.90 62.455 8.00 5.00 3.455 325 atgcatcgatcgatgatagtagc 455 23 0 43.48 60.469 12.00 4.00 3.469 326 agcatcgcgtatcagtcag 490 19 0 52.63 57.520 4.00 1.00 3.480 327 catcgcgtatcagtcagct 488 19 0 52.63 57.520 4.00 4.00 3.480 328 tagctagcatcgatgatgatca 538 22 0 40.91 58.509 8.00 7.00 3.491 329 tagcatcgatgatgatcagcta 534 22 0 40.91 58.509 8.00 8.00 3.491 330 agctagctagcatcgcgtatca 498 22 0 50.00 61.508 10.00 1.00 3.508 331 gctagctagcatcgcgtatcag 497 22 0 54.55 61.508 10.00 2.00 3.508 332 ctagctagcatcgatgatgatca 539 23 0 43.48 59.492 8.00 7.00 3.508 333 tagctagcatcgatgatgatcag 538 23 0 43.48 59.492 8.00 6.00 3.508 334 ctagcatcgatgatgatcagcta 535 23 0 43.48 59.492 8.00 8.00 3.508 335 tagcatcgatgatgatcagctag 534 23 0 43.48 59.492 8.00 5.00 3.508 336 catcgatgatgatcagctagcta 531 23 0 43.48 59.492 8.00 6.00 3.508 337 tagctagcatgatgatcgatcag 291 23 0 43.48 59.492 10.00 8.00 3.508 338 agctagctagatcgccgc 597 18 0 61.11 58.478 8.00 3.00 3.522 339 gctagctagatcgccgct 596 18 0 61.11 58.478 8.00 3.00 3.522 340 ctagccgcacatcgatcg 100 18 0 61.11 61.523 6.00 6.00 3.523 341 gcatcgatgatcgatagctact 559 22 0 45.45 58.470 11.00 4.00 3.530 342 gcatcgatcgatgatagtagct 453 22 0 45.45 58.470 12.00 4.00 3.530 343 acgatcgatcagctagctagca 578 22 0 50.00 61.537 10.00 8.00 3.537 344 tcgcgcagagatgatagtagct 375 22 0 50.00 61.537 4.00 4.00 3.537 345 agctacgatcgatcagctagtca 619 23 0 47.83 60.556 8.00 5.00 3.556 346 gctacgatcgatcagctagtcag 618 23 0 52.17 60.556 8.00 4.00 3.556 347 ctacgatcgatcagctagtcagc 617 23 0 52.17 60.556 8.00 3.00 3.556 348 tacgatcgatcagctagtcagct 616 23 0 47.83 60.556 8.00 5.00 3.556 349 acgatcgatcagctagtcagcta 615 23 0 47.83 60.556 8.00 3.00 3.556 350 gcatgatcgactagctagctacg 635 23 0 52.17 60.560 10.00 8.00 3.560 351 ctagctagcatcgcgtatcagtc 496 23 0 52.17 60.560 8.00 1.00 3.560 352 atcgcgtatcagtcagctagcta 487 23 0 47.83 60.560 8.00 6.00 3.560 353 gcatcgatgatcgatagctac 559 21 0 47.62 57.434 11.00 4.00 3.566 354 gcatcgatcgatgatagtagc 453 21 0 47.62 57.434 12.00 4.00 3.566 355 agcccagctagctagcatc 678 19 0 57.89 57.433 10.00 6.00 3.567 356 tcgatcgatcagtcagctagct 128 22 0 50.00 61.569 8.00 8.00 3.569 357 atgcgctagctagtagccc 692 19 0 57.89 57.405 8.00 8.00 3.595 358 atcagctagctagcatcgatg 571 21 0 47.62 57.405 10.00 8.00 3.595 359 cagctagctagcatcgatgat 569 21 0 47.62 57.405 10.00 8.00 3.595 360 tagtagcccagctagctagcatc 682 23 0 52.17 59.387 10.00 6.00 3.613 361 tagctagcatcgatcgcgc 388 19 0 57.89 62.627 6.00 4.00 3.627 362 tcgcctagctagcatcga 423 18 0 55.56 58.362 8.00 4.00 3.638 363 cgcgtatcagtcagctagc 485 19 0 57.89 57.357 6.00 6.00 3.643 364 gatcgatcagctagctagcatc 576 22 0 50.00 58.355 10.00 8.00 3.645 365 cgcgcagagatgatagtagctag 374 23 0 52.17 60.675 6.00 4.00 3.675 366 cgatcgatcagctagtcagctag 614 23 0 52.17 60.675 8.00 5.00 3.675 367 cgatcgatcagtcagctagctag 127 23 0 52.17 60.675 8.00 8.00 3.675 368 tagtagcccagctagctagca 682 21 0 52.38 57.324 10.00 8.00 3.676 369 ctagtagcccagctagctagca 683 22 0 54.55 58.309 10.00 8.00 3.691 370 atcgccgctacgatcgat 587 18 0 55.56 61.698 6.00 6.00 3.698 371 ctagctagccgcacatcg 104 18 0 61.11 58.288 8.00 2.00 3.712 372 tgatcgatcagtcagctagctaa 279 23 0 43.48 59.284 10.00 8.00 3.716 373 cgatcgcgcagagatgatagt 378 21 0 52.38 62.718 6.00 1.00 3.718 374 cctagctagcatcgatcgatca 420 22 0 50.00 61.720 10.00 6.00 3.720 375 atgatgatcgatcagtcagct 283 21 0 42.86 57.267 12.00 6.00 3.733 376 ctagctagctagcatcgatcg 393 21 0 52.38 57.266 12.00 6.00 3.734 377 tagctagtagcatgcatgcatc 470 22 0 45.45 58.265 10.00 8.00 3.735 378 agcccagctagctagcatcgt 678 21 0 57.14 62.744 10.00 6.00 3.744 379 atcgcgcagagatgatagtag 376 21 0 47.62 57.255 4.00 1.00 3.745 380 atcgatgtagcatcgatcagt 330 21 0 42.86 57.244 12.00 2.00 3.756 381 tagctagctagcatcgcgtat 499 21 0 47.62 57.241 10.00 4.00 3.759 382 ctagctagtagcatgcatgcatc 471 23 0 47.83 59.227 10.00 8.00 3.773 383 ctagctagctagcatcgcgtat 500 22 0 50.00 58.212 12.00 4.00 3.788 384 ctagctagctagccgcaca 108 19 0 57.89 57.208 12.00 1.00 3.792 385 cgatgtagcatcgatcagtagct 328 23 0 47.83 60.793 8.00 4.00 3.793 386 atgatcgatcagtcagctagct 280 22 0 45.45 58.201 10.00 8.00 3.799 387 ctagcatcgatcgcgcag 385 18 0 61.11 61.813 6.00 4.00 3.813 388 agcatcgatcgatcagtcagc 132 21 0 52.38 62.815 10.00 2.00 3.815 389 gcatcgatcgatcagtcagct 131 21 0 52.38 62.815 10.00 4.00 3.815 390 agcatcgatgatgatcagc 533 19 0 47.37 57.176 8.00 7.00 3.824 391 gcatcgatgatgatcagct 532 19 0 47.37 57.176 8.00 5.00 3.824 392 cccagctagctagcatcgtca 676 21 0 57.14 62.839 10.00 2.00 3.839 393 atcgatcgatgtagcatcg 334 19 0 47.37 57.160 10.00 8.00 3.840 394 tactagctagcatcgatcgatca 140 23 0 43.48 59.159 10.00 6.00 3.841 395 gcatcgatcgatgtagcat 336 19 0 47.37 57.155 12.00 4.00 3.845 396 tagcatcgatcgatcagctagc 414 22 0 50.00 61.859 10.00 8.00 3.859 397 gcatcgatcgatcagctagcta 412 22 0 50.00 61.859 10.00 6.00 3.859 398 gctagcatactagcatcgatcga 348 23 0 47.83 60.866 10.00 8.00 3.866 399 tagctagcatcgatcgatcagtc 137 23 0 47.83 60.873 10.00 6.00 3.873 400 agctactagctagcatcgatcg 143 22 0 50.00 58.126 9.00 6.00 3.874 401 atcgatcgatcagtcagctag 129 21 0 47.62 57.122 10.00 4.00 3.878 402 tagctagctagccgcacatcg 107 21 0 57.14 62.882 10.00 2.00 3.882 403 atgatcgatcagtcagctagc 280 21 0 47.62 57.117 10.00 6.00 3.883 404 gctactagctagcatcgatcg 142 21 0 52.38 57.116 8.00 6.00 3.884 405 cagctagctagtagcatgcatg 474 22 0 50.00 58.085 8.00 8.00 3.915 406 tgatgatcgatcagtcagctag 282 22 0 45.45 58.076 12.00 4.00 3.924 407 atcgatcagctagtcagctagc 612 22 0 50.00 58.034 7.00 7.00 3.966 408 ctagctagtagcatgcatgca 471 21 0 47.62 57.032 10.00 8.00 3.968 409 tcgatgtagcatcgatcagtag 329 22 0 45.45 58.026 10.00 0.00 3.974 410 agctagcatcgatcgatca 417 19 0 47.37 57.026 10.00 6.00 3.974 411 gctagcatcgatcgatcag 416 19 0 52.63 57.026 10.00 8.00 3.974 412 ctagcatcgatcgatcagc 415 19 0 52.63 57.026 10.00 6.00 3.974 413 tagcatcgatcgatcagct 414 19 0 47.37 57.026 10.00 8.00 3.974 414 agcatcgatcgatcagcta 413 19 0 47.37 57.026 10.00 6.00 3.974 415 gcatcgatcgatcagctag 412 19 0 52.63 57.026 10.00 5.00 3.974 416 catcgatcgatcagctagc 411 19 0 52.63 57.026 10.00 6.00 3.974 417 gatcgcgcagagatgatag 377 19 0 52.63 57.026 4.00 0.00 3.974 418 agctagcatcgatcgatca 136 19 0 47.37 57.026 10.00 6.00 3.974 419 gctagcatcgatcgatcag 135 19 0 52.63 57.026 10.00 8.00 3.974 420 gcatcgatgatcagctagc 208 19 0 52.63 57.021 8.00 6.00 3.979 421 gatcagctagctagcatcgatga 572 23 0 47.83 61.000 10.00 8.00 4.000 422 tcagctagctagcatcgatgatc 570 23 0 47.83 61.000 10.00 6.00 4.000 423 cgactagctagctacgatcgat 628 22 0 50.00 57.990 10.00 6.00 4.010 424 tgatcagctagctagtagcatcg 225 23 0 47.83 58.981 8.00 6.00 4.019 425 tcagctagctagtagcatcgatg 222 23 0 47.83 58.981 8.00 8.00 4.019 426 cagctagctagtagcatcgatga 221 23 0 47.83 58.981 8.00 6.00 4.019 427 gatgatcgatcagtcagctagcta 281 24 0 45.83 60.034 10.00 6.00 4.034 428 ctactagctagcatcgatcgatca 141 24 0 45.83 60.049 10.00 6.00 4.049 429 tactagctagcatcgatcgatcag 140 24 0 45.83 60.049 10.00 8.00 4.049 430 gctactagctagcatcgatgatga 543 24 0 45.83 60.056 8.00 6.00 4.056 431 gctagtagctagcatgatgatcga 296 24 0 45.83 60.056 8.00 4.00 4.056 432 gctagctagtagcatcgatgatca 219 24 0 45.83 60.056 8.00 6.00 4.056 433 tagctagtagcatcgatgatcagc 217 24 0 45.83 60.056 8.00 8.00 4.056 434 gctagtagcatcgatgatcagcta 215 24 0 45.83 60.056 8.00 8.00 4.056 435 tagtagcatcgatgatcagctagc 213 24 0 45.83 60.056 8.00 6.00 4.056 436 tagctagcatactagcatcgatcg 350 24 0 45.83 60.070 10.00 6.00 4.070 437 gatagtagctagctagcatcgcc 441 23 0 52.17 58.921 12.00 6.00 4.079 438 tgatcgatcagtcagctagcta 279 22 0 45.45 57.905 10.00 6.00 4.095 439 tgcatcgatcgatgatagtagct 454 23 0 43.48 61.109 12.00 4.00 4.109 440 gtagctagctagcatcgcctagc 437 23 0 56.52 61.109 10.00 6.00 4.109 441 agctacgatcgatcagctagtc 619 22 0 50.00 57.891 8.00 6.00 4.109 442 gctagtcagctagctagatcgcc 604 23 0 56.52 61.123 8.00 6.00 4.123 443 gctagtcagctagctagatcgc 604 22 0 54.55 57.876 8.00 8.00 4.124 444 ctagctagatcgccgctacgat 595 22 0 54.55 62.138 8.00 6.00 4.138 445 tgtagcatcgatcagtagctagc 325 23 0 47.83 58.849 6.00 6.00 4.151 446 tagctactagctagcatcgatcga 144 24 0 45.83 59.847 11.00 8.00 4.153 447 catgatcgactagctagctacga 634 23 0 47.83 58.847 10.00 8.00 4.153 448 gctagcatcgatgatgatcagc 536 22 0 50.00 62.158 8.00 8.00 4.158 449 gcatcgatgatgatcagctagc 532 22 0 50.00 62.158 8.00 7.00 4.158 450 tagtagctagctagcatcgcct 439 22 0 50.00 57.839 10.00 4.00 4.161 451 agtagctagctagcatcgccta 438 22 0 50.00 57.839 10.00 6.00 4.161 452 gtagctagctagcatcgcctag 437 22 0 54.55 57.839 10.00 4.00 4.161 453 tagcatcgatgatcagctagctag 210 24 0 45.83 60.174 10.00 8.00 4.174 454 agctagtcagctagctagatcgc 605 23 0 52.17 58.820 9.00 8.00 4.180 455 tcgcgtatcagtcagctagctag 486 23 0 52.17 61.184 8.00 8.00 4.184 456 tagctagctagcatcgcctagct 436 23 0 52.17 61.214 10.00 8.00 4.214 457 ctagtagcccagctagctagcatc 683 24 0 54.17 60.219 10.00 6.00 4.219 458 gatagtagctagctagcatcgcct 441 24 0 50.00 59.764 12.00 4.00 4.236 459 tgtagcatcgatcagtagctagct 325 24 0 45.83 59.754 8.00 8.00 4.246 460 agtagctagctagcatcgcctag 438 23 0 52.17 58.754 10.00 4.00 4.246 461 cgcgtatcagtcagctagctagta 485 24 0 50.00 60.255 8.00 4.00 4.255 462 gtagcatgcatgcatcga 464 18 0 50.00 57.745 10.00 8.00 4.255 463 ctagtcagctagctagatcgcc 603 22 0 54.55 57.739 8.00 6.00 4.261 464 tcgatcagctagtcagctagcta 611 23 0 47.83 58.736 9.00 7.00 4.264 465 gctagcatcgcctagctagcat 430 22 0 54.55 62.268 9.00 6.00 4.268 466 gcatgcatcgatcgatgatagt 457 22 0 45.45 62.270 12.00 8.00 4.270 467 actagctagcatcgatgatgatca 540 24 0 41.67 60.271 8.00 7.00 4.271 468 gtagctagcatgatgatcgatcag 292 24 0 45.83 60.271 10.00 8.00 4.271 469 tagctagcatgatgatcgatcagt 291 24 0 41.67 60.271 10.00 8.00 4.271 470 tagtagcccagctagctagcat 682 22 0 50.00 57.705 10.00 8.00 4.295 471 cagctagctagcatcgtcagcta 674 23 0 52.17 61.306 10.00 6.00 4.306 472 gtcagctagctagtagcatgca 476 22 0 50.00 57.681 8.00 6.00 4.319 473 agctagcatcgcgtatcagtca 494 22 0 50.00 62.319 6.00 1.00 4.319 474 gctagcatcgcgtatcagtcag 493 22 0 54.55 62.319 6.00 1.00 4.319 475 ctagcatcgcgtatcagtcagc 492 22 0 54.55 62.319 4.00 2.00 4.319 476 tagcatcgcgtatcagtcagct 491 22 0 50.00 62.319 4.00 4.00 4.319 477 agcatcgcgtatcagtcagcta 490 22 0 50.00 62.319 4.00 3.00 4.319 478 gcatcgcgtatcagtcagctag 489 22 0 54.55 62.319 4.00 4.00 4.319 479 catcgcgtatcagtcagctagc 488 22 0 54.55 62.319 6.00 6.00 4.319 480 tagctagcatcgtcagctagctag 670 24 0 50.00 59.677 11.00 8.00 4.323 481 cagtcagctagctagtagcatgc 478 23 0 52.17 58.672 8.00 7.00 4.328 482 agtcagctagctagtagcatgca 477 23 0 47.83 58.672 8.00 6.00 4.328 483 tcgatcagctagtcagctagctag 611 24 0 50.00 59.644 9.00 8.00 4.356 484 tcagctagtcagctagctagatcg 607 24 0 50.00 59.644 9.00 6.00 4.356 485 ctagtagcccagctagctagcat 683 23 0 52.17 58.628 10.00 8.00 4.372 486 tcagtcagctagctagtagcatgc 479 24 0 50.00 60.373 8.00 7.00 4.373 487 gcgctagctagtagcccagcta 690 22 0 59.09 62.383 8.00 6.00 4.383 488 ctagctagcatcgatgatgatcag 539 24 0 45.83 60.391 8.00 6.00 4.391 489 catcgatgatgatcagctagctag 531 24 0 45.83 60.391 8.00 8.00 4.391 490 cgctacgatcgatcagct 582 18 0 55.56 57.606 8.00 4.00 4.394 491 atgatcgatcagtcagctagctaa 280 24 0 41.67 59.570 10.00 8.00 4.430 492 agatcgccgctacgatcg 589 18 0 61.11 62.431 8.00 6.00 4.431 493 gcatcgatgatcgatagctacta 559 23 0 43.48 58.534 11.00 4.00 4.466 494 gcatcgatcgatgatagtagcta 453 23 0 43.48 58.534 12.00 6.00 4.466 495 tagctagctagcatcgcgtatca 499 23 0 47.83 61.466 10.00 1.00 4.466 496 tacgatcgatcagctagctagca 579 23 0 47.83 61.493 10.00 8.00 4.493 497 tcgcgcagagatgatagtagcta 375 23 0 47.83 61.493 6.00 6.00 4.493 498 gcctagctagcatcgatcgatc 421 22 0 54.55 62.516 10.00 8.00 4.516 499 tcgatcgatcagtcagctagcta 128 23 0 47.83 61.523 8.00 6.00 4.523 500 tagatcgccgctacgatc 590 18 0 55.56 57.475 8.00 8.00 4.525 501 tgatcgatcagtcagctagctaaa 279 24 0 41.67 60.527 10.00 6.00 4.527 502 tagccgcacatcgatcga 99 18 0 55.56 62.543 8.00 8.00 4.543 503 agctagctagtagcatgcatgca 473 23 0 47.83 61.544 10.00 8.00 4.544 504 tagctacgatcgatcagctagtca 620 24 0 45.83 60.546 10.00 6.00 4.546 505 tacgatcgatcagctagtcagcta 616 24 0 45.83 60.546 8.00 3.00 4.546 506 atgatgatcgatcagtcagctag 283 23 0 43.48 58.451 12.00 4.00 4.549 507 actagctagctacgatcgatcagc 626 24 0 50.00 60.553 10.00 6.00 4.553 508 gcatcgatgatcgatagctactag 559 24 0 45.83 59.445 11.00 5.00 4.555 509 catcgatgatcgatagctactagc 558 24 0 45.83 59.445 11.00 7.00 4.555 510 gcatcgatcgatgatagtagctag 453 24 0 45.83 59.445 12.00 4.00 4.555 511 catcgatcgatgatagtagctagc 452 24 0 45.83 59.445 12.00 6.00 4.555 512 cagctagctagatcgccg 598 18 0 61.11 57.442 8.00 6.00 4.558 513 agcatcgcctagctagca 427 18 0 55.56 57.439 8.00 4.00 4.561 514 gctagtagcccagctagctagca 684 23 0 56.52 61.586 11.00 8.00 4.586 515 gtcagctagctagtagcatgcatg 476 24 0 50.00 60.603 8.00 8.00 4.603 516 tcgatgatgatcagctagctag 529 22 0 45.45 57.392 8.00 8.00 4.608 517 atcgatgtagcatcgatcagtag 330 23 0 43.48 58.391 12.00 0.00 4.609 518 atgatgatcgatcagtcagcta 283 22 0 40.91 57.387 12.00 4.00 4.613 519 cgatcgcgcagagatgatagta 378 22 0 50.00 62.629 6.00 3.00 4.629 520 ctagcatactagcatcgatcga 347 22 0 45.45 57.367 8.00 8.00 4.633 521 atcgatgtagcatcgatcagta 330 22 0 40.91 57.360 12.00 2.00 4.640 522 gctactagctagcatcgatgatg 543 23 0 47.83 58.360 8.00 6.00 4.640 523 gctagtagctagcatgatgatcg 296 23 0 47.83 58.360 8.00 5.00 4.640 524 tcgatgtagcatcgatcagtagc 329 23 0 47.83 61.644 10.00 2.00 4.644 525 gatgcgctagctagtagcccag 693 22 0 59.09 62.659 10.00 8.00 4.659 526 gtagcccagctagctagcatcg 680 22 0 59.09 62.659 10.00 8.00 4.659 527 tagcccagctagctagcatcgt 679 22 0 54.55 62.659 10.00 6.00 4.659 528 ctagctagctacgatcgatcagct 625 24 0 50.00 60.663 10.00 8.00 4.663 529 tgatcagctagctagtagcatcga 225 24 0 45.83 60.670 8.00 6.00 4.670 530 gctagcatgatcgactagctagct 639 24 0 50.00 60.672 10.00 8.00 4.672 531 gcgcagagatgatagtagctagct 373 24 0 50.00 60.672 8.00 8.00 4.672 532 agctagtagcatgcatgcatcg 469 22 0 50.00 62.673 10.00 8.00 4.673 533 acgatcgatcagctagctagcat 578 23 0 47.83 61.711 10.00 8.00 4.711 534 atcgcgcagagatgatagtagct 376 23 0 47.83 61.711 4.00 4.00 4.711 535 gctagcatcgatcgatcagtca 135 22 0 50.00 62.719 10.00 4.00 4.719 536 tagcatcgatcgatcagtcagc 133 22 0 50.00 62.719 10.00 2.00 4.719 537 gcatcgatcgatcagtcagcta 131 22 0 50.00 62.719 10.00 2.00 4.719 538 cccagctagctagcatcg 676 18 0 61.11 57.281 10.00 8.00 4.719 539 agctagcatcgatcgcgc 387 18 0 61.11 62.722 6.00 4.00 4.722 540 tcgatgatgatcagctagctagt 529 23 0 43.48 58.277 8.00 6.00 4.723 541 atgatcgatcagtcagctagcta 280 23 0 43.48 58.277 10.00 6.00 4.723 542 agctactagctagcatcgatgatg 544 24 0 45.83 59.274 9.00 6.00 4.726 543 agctagtagctagcatgatgatcg 297 24 0 45.83 59.274 9.00 5.00 4.726 544 atgatcagctagctagtagcatcg 226 24 0 45.83 59.274 8.00 7.00 4.726 545 atcagctagctagtagcatcgatg 223 24 0 45.83 59.274 8.00 8.00 4.726 546 cagctagctagtagcatcgatgat 221 24 0 45.83 59.274 8.00 8.00 4.726 547 gctagctagccgcacatc 105 18 0 61.11 57.273 10.00 0.00 4.727 548 agcatcgatcgatcagctagct 413 22 0 50.00 62.732 10.00 8.00 4.732 549 atcgatcgatcagtcagctagct 129 23 0 47.83 61.747 10.00 8.00 4.747 550 atagtagctagctagcatcgcc 440 22 0 50.00 57.244 10.00 4.00 4.756 551 cagctagctagatcgccgctac 598 22 0 59.09 62.774 8.00 2.00 4.774 552 cgatgtagcatcgatcagtagcta 328 24 0 45.83 60.775 8.00 6.00 4.775 553 actagctagcatcgatcgatcagt 139 24 0 45.83 60.775 10.00 6.00 4.775 554 tgatagtagctagctagcatcgc 442 23 0 47.83 58.200 12.00 8.00 4.800 555 gctagctagcatactagcatcga 352 23 0 47.83 58.200 10.00 7.00 4.800 556 cgatagctactagctagcatcga 548 23 0 47.83 58.200 12.00 8.00 4.800 557 tagctactagctagcatcgatcg 144 23 0 47.83 58.200 11.00 6.00 4.800 558 cagctagctagtagcatcgatg 221 22 0 50.00 57.193 8.00 8.00 4.807 559 tgatgatcgatcagtcagctagc 282 23 0 47.83 61.809 12.00 6.00 4.809 560 tcagctagctagtagcatgcat 475 22 0 45.45 57.189 8.00 6.00 4.811 561 atagtagctagctagcatcgcct 440 23 0 47.83 58.177 10.00 4.00 4.823 562 tagcatcgatcgcgcaga 384 18 0 55.56 62.829 6.00 4.00 4.829 563 gctactagctagcatcgatcgatc 142 24 0 50.00 60.846 10.00 8.00 4.846 564 atgtagcatcgatcagtagctagc 326 24 0 45.83 59.150 6.00 6.00 4.850 565 tagcatgcatgcatcgat 463 18 0 44.44 57.148 10.00 8.00 4.852 566 gatcagctagctagtagcatcga 224 23 0 47.83 58.113 8.00 6.00 4.887 567 agctagcatcgcctagctagca 431 22 0 54.55 62.888 9.00 7.00 4.888 568 agtagcatcgatgatcagctagct 212 24 0 45.83 60.897 8.00 8.00 4.897 569 agctagctagcatactagcatcga 353 24 0 45.83 59.095 10.00 6.00 4.905 570 tcgatcgcgcagagatga 379 18 0 55.56 62.924 6.00 4.00 4.924 571 ctagtcagctagctagatcgccg 603 23 0 56.52 61.940 8.00 6.00 4.940 572 gtcagctagctagtagcatgcat 476 23 0 47.83 58.052 8.00 6.00 4.948 573 catgatcgactagctagctacg 634 22 0 50.00 57.048 10.00 8.00 4.952 574 atcgatcagctagtcagctagcta 612 24 0 45.83 59.042 9.00 7.00 4.958 575 tagctagatcgccgctacgatc 594 22 0 54.55 62.965 8.00 8.00 4.965 576 ctagtagcatgcatgcatcgatc 467 23 0 47.83 62.001 10.00 6.00 5.001 577 atgtagcatcgatcagtagctagct 326 25 0 44.00 60.001 8.00 8.00 5.001 578 agtcagctagctagtagcatgcat 477 24 0 45.83 58.980 8.00 6.00 5.020 579 gactagctagctacgatcgatca 627 23 0 47.83 57.977 10.00 6.00 5.023 580 tagctacgatcgatcagctagtc 620 23 0 47.83 57.977 10.00 6.00 5.023 581 gatcgatcagtcagctagctaaa 278 23 0 43.48 57.951 8.00 6.00 5.049 582 cgatcagtcagctagctaaatagc 275 24 0 45.83 58.950 8.00 6.00 5.050 583 cgatcagctagtcagctagctag 610 23 0 52.17 57.942 9.00 8.00 5.058 584 cagctagtcagctagctagatcg 606 23 0 52.17 57.942 9.00 6.00 5.058 585 tagtagctagctagcatcgccta 439 23 0 47.83 57.921 10.00 6.00 5.079 586 tgcatcgatcgatgatagtagcta 454 24 0 41.67 61.080 12.00 6.00 5.080 587 gactagctagctacgatcgatcag 627 24 0 50.00 58.914 10.00 6.00 5.086 588 ctagctacgatcgatcagctagtc 621 24 0 50.00 58.914 12.00 7.00 5.086 589 gctagctagcatactagcatcgatc 352 25 0 48.00 60.089 10.00 4.00 5.089 590 atcgatcagctagtcagctagctag 612 25 0 48.00 59.895 9.00 8.00 5.105 591 atcagctagtcagctagctagatcg 608 25 0 48.00 59.895 11.00 6.00 5.105 592 catgatgatcgatcagtcagctag 284 24 0 45.83 61.155 12.00 5.00 5.155 593 gctagctagcatcgcgtatcagt 497 23 0 52.17 62.182 10.00 1.00 5.182 594 tagtagctagctagcatcgcctag 439 24 0 50.00 58.803 10.00 5.00 5.197 595 gcatgcatcgatcgatgatagta 457 23 0 43.48 62.198 12.00 6.00 5.198 596 atcagctagctagcatcgatgatc 571 24 0 45.83 61.198 10.00 6.00 5.198 597 atcgatgatgatcagctagctag 530 23 0 43.48 57.785 8.00 8.00 5.215 598 cgatcagtcagctagctaaatagct 275 25 0 44.00 59.785 8.00 8.00 5.215 599 gatagtagctagctagcatcgccta 441 25 0 48.00 59.780 12.00 6.00 5.220 600 cagtcagctagctagtagcatgca 478 24 0 50.00 61.222 8.00 6.00 5.222 601 tagctagcatcgcgtatcagtca 495 23 0 47.83 62.247 6.00 1.00 5.247 602 tagcatcgcgtatcagtcagcta 491 23 0 47.83 62.247 4.00 3.00 5.247 603 gcgtatcagtcagctagctagtagc 484 25 0 52.00 60.268 8.00 8.00 5.268 604 cagctagtcagctagctagatcgc 606 24 0 54.17 61.268 9.00 8.00 5.268 605 tactagctagcatcgatgatgatca 541 25 0 40.00 60.272 8.00 7.00 5.272 606 catcgatcgatgatagtagctagct 452 25 0 44.00 60.282 12.00 8.00 5.282 607 agctagctagcatcgcgtatcag 498 23 0 52.17 62.283 10.00 2.00 5.283 608 gctactagctagcatcgatgatgat 543 25 0 44.00 60.289 8.00 6.00 5.289 609 gctagtagctagcatgatgatcgat 296 25 0 44.00 60.289 8.00 6.00 5.289 610 atgcatcgatcgatgatagtagct 455 24 0 41.67 61.301 12.00 4.00 5.301 611 tgatagtagctagctagcatcgcc 442 24 0 50.00 61.308 12.00 6.00 5.308 612 tcgactagctagctacgatcgatc 629 24 0 50.00 61.329 12.00 8.00 5.329 613 actagctagcatcgatgatgatc 540 23 0 43.48 57.643 8.00 6.00 5.357 614 agtagctagcatgatgatcgatc 293 23 0 43.48 57.643 8.00 8.00 5.357 615 agcatgatcgactagctagctacg 636 24 0 50.00 61.368 10.00 8.00 5.368 616 atcgcgtatcagtcagctagctag 487 24 0 50.00 61.368 8.00 8.00 5.368 617 atcgatgatgatcagctagctagt 530 24 0 41.67 58.612 8.00 6.00 5.388 618 agctacgatcgatcagctagtcag 619 24 0 50.00 61.392 8.00 4.00 5.392 619 ctacgatcgatcagctagtcagct 617 24 0 50.00 61.392 8.00 5.00 5.392 620 acgatcgatcagctagtcagctag 615 24 0 50.00 61.392 8.00 5.00 5.392 621 atgatagtagctagctagcatcgc 443 24 0 45.83 58.519 12.00 8.00 5.481 622 gctagctagcatactagcatcgat 352 24 0 45.83 58.519 10.00 6.00 5.481 623 tcgatcgatgatagtagctagct 450 23 0 43.48 57.502 8.00 8.00 5.498 624 ctactagctagcatcgatcgatc 141 23 0 47.83 57.502 10.00 8.00 5.498 625 gctagctagtagcatcgatgatc 219 23 0 47.83 57.499 8.00 6.00 5.501 626 cctagctagcatcgatcgatcag 420 23 0 52.17 62.515 10.00 8.00 5.515 627 cgtatcagtcagctagctagtagca 483 25 0 48.00 59.481 8.00 6.00 5.519 628 tcgatcagtcagctagctaaatagc 276 25 0 44.00 60.527 8.00 6.00 5.527 629 gatcgcgcagagatgatagtagc 377 23 0 52.17 62.545 4.00 3.00 5.545 630 gatcagctagctagtagcatcgat 224 24 0 45.83 58.447 8.00 8.00 5.553 631 agctagctagtagcatcgatgatc 220 24 0 45.83 58.447 8.00 6.00 5.553 632 agctagctagcatactagcatcg 353 23 0 47.83 57.446 10.00 8.00 5.554 633 tagctagtagcatgcatgcatcg 470 23 0 47.83 62.589 10.00 8.00 5.589 634 atcagtcagctagctagtagcatgc 480 25 0 48.00 60.594 8.00 7.00 5.594 635 agctagctagcatactagcatcgat 353 25 0 44.00 59.362 10.00 8.00 5.638 636 cgatcgatcagctagctagcatc 577 23 0 52.17 62.647 10.00 8.00 5.647 637 agcatcgatcgatcagctagcta 413 23 0 47.83 62.647 10.00 6.00 5.647 638 gcatcgatcgatcagctagctag 412 23 0 52.17 62.647 10.00 8.00 5.647 639 tcgatgatgatcagctagctagta 529 24 0 41.67 58.348 8.00 4.00 5.652 640 gatcgatcagctagtcagctagcta 613 25 0 48.00 60.659 9.00 7.00 5.659 641 tacgatcgatcagctagctagcat 579 24 0 45.83 61.663 10.00 8.00 5.663 642 atcgcgcagagatgatagtagcta 376 24 0 45.83 61.663 6.00 6.00 5.663 643 agctagcatactagcatcgatcga 349 24 0 45.83 61.663 10.00 8.00 5.663 644 ctagctagctagcatgatcgact 646 23 0 47.83 57.335 12.00 4.00 5.665 645 agctagctagcatgatcgactag 644 23 0 47.83 57.335 10.00 8.00 5.665 646 agctagcatgatcgactagctag 640 23 0 47.83 57.335 9.00 8.00 5.665 647 ctagcatgatcgactagctagct 638 23 0 47.83 57.335 8.00 8.00 5.665 648 cgcagagatgatagtagctagct 372 23 0 47.83 57.335 8.00 8.00 5.665 649 agcatcgatcagtagctagctag 322 23 0 47.83 57.335 10.00 8.00 5.665 650 tagctactagctagcatcgatgatg 545 25 0 44.00 59.307 11.00 6.00 5.693 651 tagctagtagctagcatgatgatcg 298 25 0 44.00 59.307 11.00 5.00 5.693 652 ctagctagcatcgatcgatcagtc 138 24 0 50.00 61.697 10.00 6.00 5.697 653 atcgatcgatcagtcagctagcta 129 24 0 45.83 61.697 10.00 6.00 5.697 654 gatcgatcagtcagctagctaaat 278 24 0 41.67 58.295 8.00 8.00 5.705 655 gatgatgatcagctagctagtagca 230 25 0 44.00 59.265 8.00 6.00 5.735 656 tcgatgatgatcagctagctagtag 529 25 0 44.00 59.261 8.00 6.00 5.739 657 atgatcgatcagtcagctagctaaa 280 25 0 40.00 60.741 10.00 6.00 5.741 658 tgatcgatcagtcagctagctaaat 279 25 0 40.00 60.741 10.00 8.00 5.741 659 gctagtagcccagctagctagcat 684 24 0 54.17 61.742 11.00 8.00 5.742 660 atagtagctagctagcatcgccta 440 24 0 45.83 58.244 10.00 6.00 5.756 661 tactagctagcatcgatcgatcagt 140 25 0 44.00 60.759 10.00 6.00 5.759 662 gatgtagcatcgatcagtagctagc 327 25 0 48.00 60.768 6.00 6.00 5.768 663 tagctagcatcgcctagctagca 432 23 0 52.17 62.802 10.00 7.00 5.802 664 actagctagctacgatcgatcag 626 23 0 47.83 57.197 10.00 6.00 5.803 665 ctagctacgatcgatcagctagt 621 23 0 47.83 57.197 12.00 8.00 5.803 666 agcatgatcgactagctagctac 636 23 0 47.83 57.193 10.00 8.00 5.807 667 gtagcatcgatcagtagctagct 324 23 0 47.83 57.193 8.00 8.00 5.807 668 atcgatgtagcatcgatcagtagc 330 24 0 45.83 61.812 12.00 2.00 5.812 669 tgatgatcagctagctagtagca 228 23 0 43.48 57.177 8.00 6.00 5.823 670 agtagctagctagcatcgcctagc 438 24 0 54.17 61.851 10.00 6.00 5.851 671 gtagctagctagcatcgcctagct 437 24 0 54.17 61.851 10.00 8.00 5.851 672 cgatagctactagctagcatcgatg 548 25 0 48.00 60.861 12.00 8.00 5.861 673 ctagctagcatactagcatcgatcg 351 25 0 48.00 60.861 10.00 6.00 5.861 674 tagctagctagcatactagcatcga 354 25 0 44.00 59.133 10.00 8.00 5.867 675 ctactagctagcatcgatcgatcag 141 25 0 48.00 60.867 10.00 8.00 5.867 676 tcgcgtatcagtcagctagctagt 486 24 0 50.00 61.869 8.00 6.00 5.869 677 agctactagctagcatcgatgatga 544 25 0 44.00 60.876 9.00 6.00 5.876 678 cgatgatgatcagctagctagtagc 528 25 0 48.00 60.876 8.00 8.00 5.876 679 agctagtagctagcatgatgatcga 297 25 0 44.00 60.876 9.00 4.00 5.876 680 gatgatcagctagctagtagcatcg 227 25 0 48.00 60.876 10.00 8.00 5.876 681 atgatcagctagctagtagcatcga 226 25 0 44.00 60.876 8.00 7.00 5.876 682 tgatcagctagctagtagcatcgat 225 25 0 44.00 60.876 8.00 8.00 5.876 683 gatcagctagctagtagcatcgatg 224 25 0 48.00 60.876 8.00 8.00 5.876 684 tcagctagctagtagcatcgatgat 222 25 0 44.00 60.876 9.00 8.00 5.876 685 cagctagctagtagcatcgatgatc 221 25 0 48.00 60.876 8.00 6.00 5.876 686 agctagctagtagcatcgatgatca 220 25 0 44.00 60.876 8.00 6.00 5.876 687 gctagctagtagcatcgatgatcag 219 25 0 48.00 60.876 8.00 6.00 5.876 688 ctagctagtagcatcgatgatcagc 218 25 0 48.00 60.876 8.00 8.00 5.876 689 ctagtagcatcgatgatcagctagc 214 25 0 48.00 60.876 10.00 6.00 5.876 690 tagtagcatcgatgatcagctagct 213 25 0 44.00 60.876 8.00 8.00 5.876 691 gtagcatcgatgatcagctagctag 211 25 0 48.00 60.876 10.00 8.00 5.876 692 agctagtcagctagctagatcgcc 605 24 0 54.17 61.888 9.00 6.00 5.888 693 atagtagctagctagcatcgcctag 440 25 0 48.00 59.075 10.00 4.00 5.925 694 agctagcatcgatgatgatcagc 537 23 0 47.83 62.960 8.00 8.00 5.960 695 gctagcatcgatgatgatcagct 536 23 0 47.83 62.960 8.00 6.00 5.960 696 agcatcgatgatgatcagctagc 533 23 0 47.83 62.960 8.00 7.00 5.960 697 gcatcgatgatgatcagctagct 532 23 0 47.83 62.960 8.00 8.00 5.960 698 atgatgatcgatcagtcagctagc 283 24 0 45.83 61.975 12.00 6.00 5.975 699 agctagcatcgcctagctagcat 431 23 0 52.17 62.989 9.00 7.00 5.989 700 gtagctagcatgatgatcgatcagt 292 25 0 44.00 60.996 10.00 8.00 5.996 701 cgcgtatcagtcagctagctagtag 485 25 0 52.00 61.048 8.00 6.00 6.048 702 ctagctagctagcatactagcatcga 355 26 0 46.15 59.931 12.00 8.00 6.069 703 cagctagctagcatcgtcagctag 674 24 0 54.17 62.086 10.00 8.00 6.086 704 actagctagcatcgatgatgatcag 540 25 0 44.00 61.106 8.00 6.00 6.106 705 catcgatgatgatcagctagctagt 531 25 0 44.00 61.106 8.00 6.00 6.106 706 agtagctagcatgatgatcgatcag 293 25 0 44.00 61.106 10.00 8.00 6.106 707 agatgatgatcagctagctagtagca 231 26 0 42.31 60.108 9.00 6.00 6.108 708 atcgatcgatgatagtagctagct 451 24 0 41.67 57.861 10.00 8.00 6.139 709 cgactagctagctacgatcgatca 628 24 0 50.00 62.155 10.00 6.00 6.155 710 gcatgatcgactagctagctacga 635 24 0 50.00 62.168 10.00 8.00 6.168 711 gatgatcgatcagtcagctagctaa 281 25 0 44.00 61.208 10.00 8.00 6.208 712 ctagctagctagcatcgcgtatca 500 24 0 50.00 62.218 12.00 1.00 6.218 713 tagctagctagcatcgcgtatcag 499 24 0 50.00 62.218 10.00 2.00 6.218 714 tactagctagcatcgatgatgatc 541 24 0 41.67 57.735 8.00 6.00 6.265 715 tagtagctagcatgatgatcgatc 294 24 0 41.67 57.735 8.00 8.00 6.265 716 atgcatcgatcgatgatagtagcta 455 25 0 40.00 61.267 12.00 6.00 6.267 717 cgtatcagtcagctagctagtagcat 483 26 0 46.15 59.732 8.00 6.00 6.268 718 ctacgatcgatcagctagctagca 580 24 0 50.00 62.269 10.00 8.00 6.269 719 tcgcgcagagatgatagtagctag 375 24 0 50.00 62.269 6.00 4.00 6.269 720 tcgatcgatcagtcagctagctag 128 24 0 50.00 62.323 8.00 8.00 6.323 721 actagctagctacgatcgatcagct 626 25 0 48.00 61.329 10.00 8.00 6.329 722 ctactagctagcatcgatgatgatc 542 25 0 44.00 58.669 8.00 6.00 6.331 723 atcgatgatgatcagctagctagta 530 25 0 40.00 58.669 8.00 4.00 6.331 724 ctagtagctagcatgatgatcgatc 295 25 0 44.00 58.669 8.00 8.00 6.331 725 tagcatgatcgactagctagctacg 637 25 0 48.00 61.333 10.00 8.00 6.333 726 ctagctacgatcgatcagctagtca 621 25 0 48.00 61.355 12.00 7.00 6.355 727 tagctacgatcgatcagctagtcag 620 25 0 48.00 61.355 10.00 4.00 6.355 728 ctacgatcgatcagctagtcagcta 617 25 0 48.00 61.355 8.00 3.00 6.355 729 tacgatcgatcagctagtcagctag 616 25 0 48.00 61.355 8.00 5.00 6.355 730 cagtcagctagctagtagcatgcat 478 25 0 48.00 61.403 8.00 6.00 6.403 731 agtcagctagctagtagcatgcatg 477 25 0 48.00 61.403 8.00 8.00 6.403 732 cgatgatgatcagctagctagtag 528 24 0 45.83 57.570 8.00 6.00 6.430 733 ctagctagtagcatcgatgatcag 218 24 0 45.83 57.570 8.00 6.00 6.430 734 atgatgatcagctagctagtagca 229 24 0 41.67 57.568 8.00 6.00 6.432 735 tgatgatcagctagctagtagcat 228 24 0 41.67 57.568 8.00 8.00 6.432 736 atagctactagctagcatcgatgatg 546 26 0 42.31 59.563 11.00 6.00 6.437 737 tcgatgtagcatcgatcagtagct 329 24 0 45.83 62.441 10.00 4.00 6.441 738 tagctagctagcatactagcatcg 354 24 0 45.83 57.539 10.00 8.00 6.461 739 gatgatgatcagctagctagtagcat 230 26 0 42.31 59.532 8.00 8.00 6.468 740 atcgatgatgatcagctagctagtag 530 26 0 42.31 59.527 8.00 6.00 6.473 741 atgatagtagctagctagcatcgcc 443 25 0 48.00 61.475 12.00 6.00 6.475 742 gatagctactagctagcatcgatga 547 25 0 44.00 58.507 11.00 6.00 6.493 743 tcgatcgatgatagtagctagctag 450 25 0 44.00 58.506 10.00 8.00 6.494 744 gctagctagtagcatgcatgcatc 472 24 0 50.00 62.511 10.00 8.00 6.511 745 gatagtagctagctagcatcgcctag 441 26 0 50.00 60.535 12.00 4.00 6.535 746 ctagctagctagcatgatcgacta 646 24 0 45.83 57.434 12.00 6.00 6.566 747 tagctagctagcatgatcgactag 645 24 0 45.83 57.434 10.00 8.00 6.566 748 tagctagcatgatcgactagctag 641 24 0 45.83 57.434 11.00 8.00 6.566 749 tagcatcgatcagtagctagctag 323 24 0 45.83 57.434 10.00 8.00 6.566 750 gatgtagcatcgatcagtagctag 327 24 0 45.83 57.431 6.00 4.00 6.569 751 cgatgtagcatcgatcagtagctag 328 25 0 48.00 61.570 8.00 4.00 6.570 752 tgtagcatcgatcagtagctagctag 325 26 0 46.15 60.576 10.00 8.00 6.576 753 ctagctagctagcatactagcatcg 355 25 0 48.00 58.419 12.00 8.00 6.581 754 tatcagtcagctagctagtagcatgc 481 26 0 46.15 60.584 8.00 7.00 6.584 755 tagctagctagcatactagcatcgat 354 26 0 42.31 59.390 10.00 8.00 6.610 756 agctactagctagcatcgatcgatc 143 25 0 48.00 61.611 10.00 8.00 6.611 757 tagctagcatactagcatcgatcga 350 25 0 44.00 61.618 10.00 8.00 6.618 758 tgatgatcgatcagtcagctagct 282 24 0 45.83 62.625 12.00 8.00 6.625 759 gatcgatcagtcagctagctaaata 278 25 0 40.00 58.360 8.00 6.00 6.640 760 ctagctagctagcatgatcgactag 646 25 0 48.00 58.347 12.00 8.00 6.653 761 cgcagagatgatagtagctagctag 372 25 0 48.00 58.347 10.00 8.00 6.653 762 tagcatgatcgactagctagctac 637 24 0 45.83 57.298 10.00 8.00 6.702 763 tcgatcagtcagctagctaaatag 276 24 0 41.67 57.276 8.00 6.00 6.724 764 atcgatcagtcagctagctaaatagc 277 26 0 42.31 60.726 8.00 6.00 6.726 765 tgatcgatcagtcagctagctaaata 279 26 0 38.46 60.727 10.00 6.00 6.727 766 atcagtcagctagctagtagcatg 480 24 0 45.83 57.265 8.00 8.00 6.735 767 ctagcatgatcgactagctagctac 638 25 0 48.00 58.222 10.00 8.00 6.778 768 gtagcatcgatcagtagctagctag 324 25 0 48.00 58.222 10.00 8.00 6.778 769 gatcgatcagtcagctagctaaatag 278 26 0 42.31 59.213 8.00 6.00 6.787 770 tagtagctagctagcatcgcctagc 439 25 0 52.00 61.803 10.00 6.00 6.803 771 tcgcgtatcagtcagctagctagta 486 25 0 48.00 61.817 8.00 4.00 6.817 772 agctagctagcatactagcatcgatc 353 26 0 46.15 60.851 10.00 4.00 6.851 773 tagctactagctagcatcgatgatga 545 26 0 42.31 60.857 11.00 6.00 6.857 774 tagctagtagctagcatgatgatcga 298 26 0 42.31 60.857 11.00 4.00 6.857 775 tgcatcgatcgatgatagtagctag 454 25 0 44.00 61.864 12.00 4.00 6.864 776 tagctagcatcgatgatgatcagc 538 24 0 45.83 62.867 8.00 8.00 6.867 777 gctagcatcgatgatgatcagcta 536 24 0 45.83 62.867 8.00 8.00 6.867 778 tagcatcgatgatgatcagctagc 534 24 0 45.83 62.867 8.00 7.00 6.867 779 gcatcgatgatgatcagctagcta 532 24 0 45.83 62.867 8.00 6.00 6.867 780 tagctagcatcgcctagctagcat 432 24 0 50.00 62.904 10.00 7.00 6.904 781 agctagctagcatcgcgtatcagt 498 24 0 50.00 62.905 10.00 1.00 6.905 782 atgatcgatcagtcagctagctaaat 280 26 0 38.46 60.936 10.00 8.00 6.936 783 gcatgcatcgatcgatgatagtag 457 24 0 45.83 62.969 12.00 6.00 6.969 784 catgcatcgatcgatgatagtagc 456 24 0 45.83 62.969 12.00 4.00 6.969 785 gcgtatcagtcagctagctagtag 484 24 0 50.00 57.004 8.00 6.00 6.996 786 cgtatcagtcagctagctagtagc 483 24 0 50.00 57.004 8.00 8.00 6.996 787 ctagctagcatcgcgtatcagtca 496 24 0 50.00 62.998 8.00 1.00 6.998 788 tagctagcatcgcgtatcagtcag 495 24 0 50.00 62.998 6.00 1.00 6.998 789 ctagcatcgcgtatcagtcagcta 492 24 0 50.00 62.998 4.00 3.00 6.998 790 tagcatcgcgtatcagtcagctag 491 24 0 50.00 62.998 4.00 4.00 6.998 791 catcgcgtatcagtcagctagcta 488 24 0 50.00 62.998 8.00 6.00 6.998 792 aaagctactagctagatgatgatcagc 179 27 0 40.74 60.007 9.00 8.00 7.007 793 atcgcgtatcagtcagctagctagt 487 25 0 48.00 62.015 8.00 6.00 7.015 794 tgatagtagctagctagcatcgcct 442 25 0 48.00 62.039 12.00 4.00 7.039 795 agctactagctagcatcgatgatgat 544 26 0 42.31 61.064 9.00 6.00 7.064 796 agctagtagctagcatgatgatcgat 297 26 0 42.31 61.064 9.00 6.00 7.064 797 atgatcagctagctagtagcatcgat 226 26 0 42.31 61.064 8.00 8.00 7.064 798 atgatgatcagctagctagtagcat 229 25 0 40.00 57.922 8.00 8.00 7.078 799 ctactagctagcatcgatgatgatca 542 26 0 42.31 61.079 8.00 7.00 7.079 800 tactagctagcatcgatgatgatcag 541 26 0 42.31 61.079 8.00 6.00 7.079 801 catcgatgatgatcagctagctagta 531 26 0 42.31 61.079 8.00 4.00 7.079 802 tagtagctagcatgatgatcgatcag 294 26 0 42.31 61.079 10.00 8.00 7.079 803 gactagctagctacgatcgatcagc 627 25 0 52.00 62.095 10.00 6.00 7.095 804 aagctactagctagatgatgatcagc 178 26 0 42.31 58.900 9.00 8.00 7.100 805 tagatgatgatcagctagctagtagca 232 27 0 40.74 60.113 11.00 6.00 7.113 806 ctagctagctagcatactagcatcgat 355 27 0 44.44 60.145 12.00 8.00 7.145 807 gatagctactagctagcatcgatgat 547 26 0 42.31 58.799 11.00 8.00 7.201 808 atcgatcgatgatagtagctagctag 451 26 0 42.31 58.797 10.00 8.00 7.203 809 tcgatcagtcagctagctaaatagct 276 26 0 42.31 61.281 8.00 8.00 7.281 810 gctagcatgatcgactagctagctac 639 26 0 50.00 61.306 10.00 8.00 7.306 811 agatgatgatcagctagctagtagc 231 25 0 44.00 57.687 9.00 8.00 7.313 812 agctactagctagatgatgatcagc 177 25 0 44.00 57.687 9.00 8.00 7.313 813 agatgatgatcagctagctagtagcat 231 27 0 40.74 60.327 9.00 8.00 7.327 814 actagctagcatcgatcgatcagtc 139 25 0 48.00 62.355 10.00 6.00 7.355 815 atcgatcagtcagctagctaaatag 277 25 0 40.00 57.634 8.00 6.00 7.366 816 tcgatgtagcatcgatcagtagcta 329 25 0 44.00 62.368 10.00 6.00 7.368 817 ctacgatcgatcagctagctagcat 580 25 0 48.00 62.397 10.00 8.00 7.397 818 atcgcgcagagatgatagtagctag 376 25 0 48.00 62.397 6.00 4.00 7.397 819 gcgcagagatgatagtagctagctag 373 26 0 50.00 61.404 10.00 8.00 7.404 820 gatcgatcagctagtcagctagctag 613 26 0 50.00 61.427 9.00 8.00 7.427 821 atcgatcgatcagtcagctagctag 129 25 0 48.00 62.453 10.00 8.00 7.453 822 ctactagctagcatcgatcgatcagt 141 26 0 46.15 61.522 10.00 6.00 7.522 823 gatgtagcatcgatcagtagctagct 327 26 0 46.15 61.533 8.00 8.00 7.533 824 agtagctagctagcatcgcctagct 438 25 0 52.00 62.538 10.00 8.00 7.538 825 tgatgatcgatcagtcagctagcta 282 25 0 44.00 62.543 12.00 6.00 7.543 826 atcgatgtagcatcgatcagtagct 330 25 0 44.00 62.565 12.00 4.00 7.565 827 tagctactagctagcatcgatcgatc 144 26 0 46.15 61.570 11.00 8.00 7.570 828 gcatcgatgatcgatagctactagc 559 25 0 48.00 62.588 11.00 7.00 7.588 829 gcatcgatcgatgatagtagctagc 453 25 0 48.00 62.588 12.00 6.00 7.588 830 agctagctagtagcatcgatgatcag 220 26 0 46.15 61.633 8.00 6.00 7.633 831 ctagtagcatcgatgatcagctagct 214 26 0 46.15 61.633 10.00 8.00 7.633 832 agtagcatcgatgatcagctagctag 212 26 0 46.15 61.633 10.00 8.00 7.633 833 tatcagtcagctagctagtagcatg 481 25 0 44.00 57.367 8.00 8.00 7.633 834 tgatgatgatagtagatgatgatcagc 244 27 0 37.04 60.705 8.00 7.00 7.705 835 atgatgatcgatcagtcagctagct 283 25 0 44.00 62.743 12.00 8.00 7.743 836 atgtagcatcgatcagtagctagctag 326 27 0 44.44 60.771 10.00 8.00 7.771 837 atagctactagctagcatcgatgat 546 25 0 40.00 57.227 11.00 8.00 7.773 838 agtagctagcatgatgatcgatcagt 293 26 0 42.31 61.773 10.00 8.00 7.773 839 gatgatgatagtagatgatgatcagc 243 26 0 38.46 58.224 7.00 7.00 7.776 840 tcagctagtcagctagctagatcgc 607 25 0 52.00 62.795 9.00 8.00 7.795 841 tcagtcagctagctagtagcatgca 479 25 0 48.00 62.811 8.00 6.00 7.811 842 gctactagctagcatcgatgatgatc 543 26 0 46.15 61.816 8.00 6.00 7.816 843 gctagtagctagcatgatgatcgatc 296 26 0 46.15 61.816 8.00 8.00 7.816 844 tagctagctagcatcgcgtatcagt 499 25 0 48.00 62.822 10.00 1.00 7.822 845 tagctagctagcatactagcatcgatc 354 27 0 44.44 60.834 10.00 7.00 7.834 846 gtatcagtcagctagctagtagcatg 482 26 0 46.15 58.154 8.00 8.00 7.846 847 cgactagctagctacgatcgatcag 628 25 0 52.00 62.876 10.00 6.00 7.876 848 agcatgatcgactagctagctacga 636 25 0 48.00 62.891 10.00 8.00 7.891 849 ctagctagctagcatcgcgtatcag 500 25 0 52.00 62.912 12.00 2.00 7.912 850 atgatcgatcagtcagctagctaaata 280 27 0 37.04 60.916 10.00 6.00 7.916 851 atagtagctagctagcatcgcctagc 440 26 0 50.00 61.939 10.00 6.00 7.939 852 atcgcgtatcagtcagctagctagta 487 26 0 46.15 61.961 8.00 4.00 7.961 853 tgatagtagctagctagcatcgccta 442 26 0 46.15 61.986 12.00 6.00 7.986 854 atgcatcgatcgatgatagtagctag 455 26 0 42.31 62.008 12.00 4.00 8.008 855 catcgatcgatgatagtagctagctag 452 27 0 44.44 61.031 12.00 8.00 8.031 856 gatagctactagctagcatcgatgatg 547 27 0 44.44 61.040 11.00 6.00 8.040 857 atagctactagctagcatcgatgatga 546 27 0 40.74 61.040 11.00 6.00 8.040 858 tagctactagctagcatcgatgatgat 545 27 0 40.74 61.040 11.00 6.00 8.040 859 tagctagtagctagcatgatgatcgat 298 27 0 40.74 61.040 11.00 6.00 8.040 860 ctagcatgatcgactagctagctacg 638 26 0 50.00 62.056 10.00 8.00 8.056 861 ctagctacgatcgatcagctagtcag 621 26 0 50.00 62.099 12.00 8.00 8.099 862 ctacgatcgatcagctagtcagctag 617 26 0 50.00 62.099 8.00 5.00 8.099 863 atgatagtagctagctagcatcgcct 443 26 0 46.15 62.168 12.00 6.00 8.168 864 tagatgatgatcagctagctagtagc 232 26 0 42.31 57.771 11.00 8.00 8.229 865 gtatcagtcagctagctagtagcatgc 482 27 0 48.15 61.231 8.00 7.00 8.231 866 gatgatcgatcagtcagctagctaaa 281 26 0 42.31 62.274 10.00 6.00 8.274 867 gcataaagctactagctagatgatga 183 26 0 38.46 57.716 11.00 5.00 8.284 868 tactagctagcatcgatcgatcagtc 140 26 0 46.15 62.288 10.00 6.00 8.288 869 cgatagctactagctagcatcgatga 548 26 0 46.15 62.331 12.00 7.00 8.331 870 ctagctagcatactagcatcgatcga 351 26 0 46.15 62.331 10.00 8.00 8.331 871 tcgatgatgatcagctagctagtagc 529 26 0 46.15 62.397 8.00 8.00 8.397 872 gatgatcagctagctagtagcatcga 227 26 0 46.15 62.397 10.00 8.00 8.397 873 tcagctagctagtagcatcgatgatc 222 26 0 46.15 62.397 9.00 6.00 8.397 874 atcgatcagtcagctagctaaatagct 277 27 0 40.74 61.441 8.00 8.00 8.441 875 agcataaagctactagctagatgatga 184 27 0 37.04 58.559 11.00 6.00 8.441 876 tgatcgatcagtcagctagctaaatag 279 27 0 40.74 61.469 10.00 6.00 8.469 877 tagtagctagctagcatcgcctagct 439 26 0 50.00 62.471 10.00 8.00 8.471 878 gtagatgatgatcagctagctagtagc 233 27 0 44.44 58.516 11.00 8.00 8.484 879 atcgatgtagcatcgatcagtagcta 330 26 0 42.31 62.492 12.00 6.00 8.492 880 tcgcgtatcagtcagctagctagtag 486 26 0 50.00 62.530 8.00 6.00 8.530 881 gcgtatcagtcagctagctagtagca 484 26 0 50.00 62.543 8.00 6.00 8.543 882 gatgatgatgatagtagatgatgatca 246 27 0 33.33 58.451 8.00 8.00 8.549 883 tagtagcatcgatgatcagctagctag 213 27 0 44.44 61.591 10.00 8.00 8.591 884 atgatgatcgatcagtcagctagcta 283 26 0 42.31 62.662 12.00 6.00 8.662 885 tgatgatgatagtagatgatgatcag 244 26 0 34.62 57.322 8.00 5.00 8.678 886 tagtagctagcatgatgatcgatcagt 294 27 0 40.74 61.726 10.00 8.00 8.726 887 gactagctagctacgatcgatcagct 627 26 0 50.00 62.792 10.00 8.00 8.792 888 tagcatgatcgactagctagctacga 637 26 0 46.15 62.809 10.00 8.00 8.809 889 ctactagctagcatcgatgatgatcag 542 27 0 44.44 61.823 8.00 6.00 8.823 890 catcgatgatgatcagctagctagtag 531 27 0 44.44 61.823 8.00 6.00 8.823 891 ctagtagctagcatgatgatcgatcag 295 27 0 44.44 61.823 10.00 8.00 8.823 892 atcagctagtcagctagctagatcgc 608 26 0 50.00 62.896 11.00 8.00 8.896 893 atcagtcagctagctagtagcatgca 480 26 0 46.15 62.915 8.00 6.00 8.915 894 tcagtcagctagctagtagcatgcat 479 26 0 46.15 62.915 8.00 6.00 8.915 895 gcataaagctactagctagatgatgat 183 27 0 37.04 58.031 11.00 4.00 8.969 896 agcataaagctactagctagatgatg 184 26 0 38.46 57.029 11.00 7.00 8.971 897 cgtatcagtcagctagctagtagcatg 483 27 0 48.15 61.978 8.00 8.00 8.978 898 agctagcatgatcgactagctagctac 640 27 0 48.15 62.004 12.00 8.00 9.004 899 atgatagtagctagctagcatcgccta 443 27 0 44.44 62.113 12.00 6.00 9.113 900 gatcgatcagtcagctagctaaatagc 278 27 0 44.44 62.155 8.00 6.00 9.155 901 atgatgatgatagtagatgatgatcag 245 27 0 33.33 57.694 8.00 6.00 9.306 902 gatgatcgatcagtcagctagctaaat 281 27 0 40.74 62.397 10.00 8.00 9.397 903 cgatagctactagctagcatcgatgat 548 27 0 44.44 62.447 12.00 8.00 9.447 904 agctactagctagcatcgatgatgatc 544 27 0 44.44 62.514 9.00 6.00 9.514 905 atcgatgatgatcagctagctagtagc 530 27 0 44.44 62.514 8.00 8.00 9.514 906 agctagtagctagcatgatgatcgatc 297 27 0 44.44 62.514 9.00 8.00 9.514 907 atcagctagctagtagcatcgatgatc 223 27 0 44.44 62.514 11.00 7.00 9.514 908 atagtagctagctagcatcgcctagct 440 27 0 48.15 62.576 10.00 8.00 9.576 909 atcgcgtatcagtcagctagctagtag 487 27 0 48.15 62.638 8.00 6.00 9.638 910 tgatagtagctagctagcatcgcctag 442 27 0 48.15 62.644 12.00 4.00 9.644 911 gcgtatcagtcagctagctagtagcat 484 27 0 48.15 62.652 8.00 6.00 9.652 912 tatcagtcagctagctagtagcatgca 481 27 0 44.44 62.833 8.00 6.00 9.833 913 ctactagctagcatcgatcgatcagtc 141 27 0 48.15 62.974 10.00 6.00 9.974 914 taaagctactagctagatgatgatcag 180 27 0 37.04 57.019 9.00 6.00 9.981 primer3-2.3.6/test/primer_list_last/SP01-02.txt.rev0000664000077300007730000000375112231156244020654 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TCCTGTTGGAGAAAAGCCAC 247 20 0 50.00 60.232 3.00 0.00 0.232 1 CCTGTTGGAGAAAAGCCACT 246 20 0 50.00 59.328 3.00 1.00 0.672 2 TTCCTGTTGGAGAAAAGCCA 248 20 0 45.00 60.745 3.00 0.00 0.745 3 TGTTGGAGAAAAGCCACTGG 244 20 0 50.00 61.204 5.00 5.00 1.204 4 CTGTTGGAGAAAAGCCACTG 245 20 0 50.00 58.496 3.00 3.00 1.504 5 TCCTGTTGGAGAAAAGCCA 247 19 0 47.37 59.354 3.00 0.00 1.646 6 TGGAGAAAAGCCACTGGC 241 18 0 55.56 59.918 7.00 7.00 2.082 7 GGAGAAAAGCCACTGGCA 240 18 0 55.56 59.918 7.00 5.00 2.082 8 GTTGGAGAAAAGCCACTGGC 243 20 0 55.00 62.088 7.00 7.00 2.088 9 TCCTGTTGGAGAAAAGCCACT 247 21 0 47.62 61.165 3.00 1.00 2.165 10 TTGGAGAAAAGCCACTGGC 242 19 0 52.63 61.313 7.00 7.00 2.313 11 TTCCTGTTGGAGAAAAGCCAC 248 21 0 47.62 61.523 3.00 0.00 2.523 12 CCTGTTGGAGAAAAGCCAC 246 19 0 52.63 58.272 3.00 0.00 2.728 13 GTTGGAGAAAAGCCACTGG 243 19 0 52.63 58.272 5.00 5.00 2.728 14 CCTGTTGGAGAAAAGCCACTG 246 21 0 52.38 62.093 3.00 3.00 3.093 15 CTGTTGGAGAAAAGCCACTGG 245 21 0 52.38 62.093 5.00 5.00 3.093 16 TTCCTGTTGGAGAAAAGCC 248 19 0 47.37 57.850 3.00 1.00 3.150 17 TGTTGGAGAAAAGCCACTG 244 19 0 47.37 57.360 3.00 3.00 3.640 18 TGGAGAAAAGCCACTGGCA 241 19 0 52.63 62.902 7.00 5.00 3.902 19 TTCCTGTTGGAGAAAAGCCACT 248 22 0 45.45 62.351 3.00 1.00 4.351 20 CCTGTTGGAGAAAAGCCA 246 18 0 50.00 57.278 3.00 0.00 4.722 21 TTGGAGAAAAGCCACTGG 242 18 0 50.00 57.278 5.00 5.00 4.722 22 GACCCCACGCACGCTAAG 158 18 0 66.67 62.798 3.00 3.00 4.798 primer3-2.3.6/test/primer_list_last/SP01-19.txt.for0000664000077300007730000001753012231156244020656 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TATTCCTGTCCCTGTGCCTC 48 20 0 55.00 60.073 2.00 1.00 0.073 1 GCCTCAGAAACCTGGCTACA 63 20 0 55.00 60.397 3.00 1.00 0.397 2 TGCCTCAGAAACCTGGCTAC 62 20 0 55.00 60.397 3.00 3.00 0.397 3 GTGCCTCAGAAACCTGGCTA 61 20 0 55.00 60.397 3.00 2.00 0.397 4 CTGTCCCTGTGCCTCAGAA 53 19 0 57.89 59.963 5.00 1.00 1.037 5 ATTCCTGTCCCTGTGCCTC 49 19 0 57.89 60.064 2.00 1.00 1.064 6 AATATTCCTGTCCCTGTGCC 46 20 0 50.00 58.864 6.00 1.00 1.136 7 AATATTCCTGTCCCTGTGCCT 46 21 0 47.62 59.841 6.00 0.00 1.159 8 CCCTGTGCCTCAGAAACCT 57 19 0 57.89 60.245 5.00 0.00 1.245 9 TGTCCCTGTGCCTCAGAAAC 54 20 0 55.00 61.259 5.00 5.00 1.259 10 ATATTCCTGTCCCTGTGCCTC 47 21 0 52.38 60.342 4.00 1.00 1.342 11 GAAACCTGGCTACAGTTCCC 69 20 0 55.00 58.646 3.00 3.00 1.354 12 AAACCTGGCTACAGTTCCCTC 70 21 0 52.38 59.630 3.00 0.00 1.370 13 GAAACCTGGCTACAGTTCCCT 69 21 0 52.38 59.630 3.00 1.00 1.370 14 AGAAACCTGGCTACAGTTCCC 68 21 0 52.38 59.630 3.00 3.00 1.370 15 TGTCCCTGTGCCTCAGAAA 54 19 0 52.63 60.385 5.00 3.00 1.385 16 CTGTCCCTGTGCCTCAGAAA 53 20 0 55.00 61.390 5.00 3.00 1.390 17 GTGCCTCAGAAACCTGGCT 61 19 0 57.89 60.402 3.00 3.00 1.402 18 TGCCTCAGAAACCTGGCTA 62 19 0 52.63 59.531 3.00 2.00 1.469 19 ATATTCCTGTCCCTGTGCCT 47 20 0 50.00 58.473 4.00 0.00 1.527 20 CTGTGCCTCAGAAACCTGG 59 19 0 57.89 59.399 5.00 3.00 1.601 21 CCTGTGCCTCAGAAACCTG 58 19 0 57.89 59.399 5.00 2.00 1.601 22 AACCTGGCTACAGTTCCCTC 71 20 0 55.00 58.255 3.00 0.00 1.745 23 GTCCCTGTGCCTCAGAAACC 55 20 0 60.00 62.024 5.00 3.00 2.024 24 TAATATTCCTGTCCCTGTGCC 45 21 0 47.62 58.917 6.00 1.00 2.083 25 TAATATTCCTGTCCCTGTGCCT 45 22 0 45.45 59.856 6.00 0.00 2.144 26 TCCCTGTGCCTCAGAAACCT 56 20 0 55.00 62.145 5.00 0.00 2.145 27 CAGAAACCTGGCTACAGTTCC 67 21 0 52.38 58.851 3.00 2.00 2.149 28 CCTCAGAAACCTGGCTACAGTT 64 22 0 50.00 59.810 3.00 2.00 2.190 29 GTGCCTCAGAAACCTGGCTAC 61 21 0 57.14 61.195 3.00 3.00 2.195 30 TTAATATTCCTGTCCCTGTGCC 44 22 0 45.45 60.204 6.00 1.00 2.204 31 AAACCTGGCTACAGTTCCCT 70 20 0 50.00 57.789 3.00 1.00 2.211 32 TCCCTGTGCCTCAGAAACC 56 19 0 57.89 61.213 5.00 1.00 2.213 33 TTCCTGTCCCTGTGCCTC 50 18 0 61.11 59.749 2.00 1.00 2.251 34 GCCTCAGAAACCTGGCTACAG 63 21 0 57.14 61.316 3.00 3.00 2.316 35 CCTGTCCCTGTGCCTCAG 52 18 0 66.67 60.405 5.00 5.00 2.405 36 CCTCAGAAACCTGGCTACAG 64 20 0 55.00 57.547 3.00 3.00 2.453 37 TGCCTCAGAAACCTGGCT 62 18 0 55.56 59.499 3.00 3.00 2.501 38 AGAAACCTGGCTACAGTTCCCT 68 22 0 50.00 60.528 3.00 2.00 2.528 39 CCTCAGAAACCTGGCTACAGT 64 21 0 52.38 58.468 3.00 3.00 2.532 40 GTGCCTCAGAAACCTGGC 61 18 0 61.11 59.345 3.00 2.00 2.655 41 TCAGAAACCTGGCTACAGTTCC 66 22 0 50.00 60.661 3.00 2.00 2.661 42 GTCCCTGTGCCTCAGAAAC 55 19 0 57.89 58.201 5.00 5.00 2.799 43 CCCTGTGCCTCAGAAACC 57 18 0 61.11 59.180 5.00 1.00 2.820 44 TATTCCTGTCCCTGTGCCT 48 19 0 52.63 58.086 2.00 0.00 2.914 45 ATTCCTGTCCCTGTGCCTCA 49 20 0 55.00 62.996 3.00 3.00 2.996 46 CTGTCCCTGTGCCTCAGAAAC 53 21 0 57.14 62.180 5.00 5.00 3.180 47 TGTCCCTGTGCCTCAGAA 54 18 0 55.56 58.812 5.00 1.00 3.188 48 GAAACCTGGCTACAGTTCCCTC 69 22 0 54.55 61.364 3.00 0.00 3.364 49 TGTGCCTCAGAAACCTGGC 60 19 0 57.89 62.397 3.00 2.00 3.397 50 ATTAATATTCCTGTCCCTGTGCC 43 23 0 43.48 60.438 6.00 1.00 3.438 51 TCCTGTCCCTGTGCCTCA 51 18 0 61.11 61.468 3.00 3.00 3.468 52 CCTGTCCCTGTGCCTCAGA 52 19 0 63.16 62.470 5.00 3.00 3.470 53 TCCTGTCCCTGTGCCTCAG 51 19 0 63.16 62.470 5.00 5.00 3.470 54 GCCTCAGAAACCTGGCTAC 63 19 0 57.89 57.465 3.00 3.00 3.535 55 AATATTCCTGTCCCTGTGCCTC 46 22 0 50.00 61.574 6.00 1.00 3.574 56 ATATTCCTGTCCCTGTGCC 47 19 0 52.63 57.370 4.00 1.00 3.630 57 CTGTCCCTGTGCCTCAGA 53 18 0 61.11 58.348 5.00 3.00 3.652 58 TGTGCCTCAGAAACCTGG 60 18 0 55.56 58.249 3.00 3.00 3.751 59 CTCAGAAACCTGGCTACAGTTC 65 22 0 50.00 58.135 3.00 2.00 3.865 60 TTCCTGTCCCTGTGCCTCA 50 19 0 57.89 62.869 3.00 3.00 3.869 61 GTCCCTGTGCCTCAGAAACCT 55 21 0 57.14 62.881 5.00 0.00 3.881 62 TATTCCTGTCCCTGTGCCTCA 48 21 0 52.38 62.891 3.00 3.00 3.891 63 TCAGAAACCTGGCTACAGTTC 66 21 0 47.62 57.065 3.00 2.00 3.935 64 ATTCCTGTCCCTGTGCCT 49 18 0 55.56 57.991 2.00 0.00 4.009 65 TTAATATTCCTGTCCCTGTGCCT 44 23 0 43.48 61.044 6.00 0.00 4.044 66 GCCTCAGAAACCTGGCTACAGT 63 22 0 54.55 62.045 3.00 3.00 4.045 67 CAGAAACCTGGCTACAGTTCCC 67 22 0 54.55 62.245 3.00 3.00 4.245 68 TATTAATATTCCTGTCCCTGTGCC 42 24 0 41.67 60.432 8.00 1.00 4.432 69 CTCAGAAACCTGGCTACAGTTCC 65 23 0 52.17 61.514 3.00 2.00 4.514 70 CCTCAGAAACCTGGCTACAGTTC 64 23 0 52.17 61.514 3.00 2.00 4.514 71 TAATATTCCTGTCCCTGTGCCTC 45 23 0 47.83 61.529 6.00 1.00 4.529 72 TCCCTGTGCCTCAGAAAC 56 18 0 55.56 57.158 5.00 3.00 4.842 73 GTCCCTGTGCCTCAGAAA 55 18 0 55.56 57.158 5.00 3.00 4.842 74 ATTAATATTCCTGTCCCTGTGC 43 22 0 40.91 57.155 6.00 2.00 4.845 75 AGAAACCTGGCTACAGTTCCCTC 68 23 0 52.17 62.164 3.00 0.00 5.164 76 ATTAATATTCCTGTCCCTGTGCCT 43 24 0 41.67 61.230 6.00 0.00 5.230 77 ATATTAATATTCCTGTCCCTGTGCC 41 25 0 40.00 60.638 10.00 1.00 5.638 78 TATTAATATTCCTGTCCCTGTGC 42 23 0 39.13 57.263 8.00 2.00 5.737 79 AAATATTAATATTCCTGTCCCTGTGC 39 26 0 34.62 59.917 12.00 2.00 6.083 80 AATATTAATATTCCTGTCCCTGTGC 40 25 0 36.00 58.823 12.00 2.00 6.177 81 TATTAATATTCCTGTCCCTGTGCCT 42 25 0 40.00 61.200 8.00 0.00 6.200 82 ATATTAATATTCCTGTCCCTGTGC 41 24 0 37.50 57.627 10.00 2.00 6.373 83 TTAATATTCCTGTCCCTGTGCCTC 44 24 0 45.83 62.607 6.00 1.00 6.607 84 CAAATATTAATATTCCTGTCCCTGTG 38 26 0 34.62 59.176 12.00 1.00 6.824 85 TCAAATATTAATATTCCTGTCCCTG 37 25 0 32.00 57.690 12.00 1.00 7.310 86 ATATTAATATTCCTGTCCCTGTGCCT 41 26 0 38.46 61.365 10.00 0.00 7.365 87 CTCAAATATTAATATTCCTGTCCCTG 36 26 0 34.62 58.540 12.00 1.00 7.460 88 TCAAATATTAATATTCCTGTCCCTGT 37 26 0 30.77 58.425 12.00 0.00 7.575 89 TCAAATATTAATATTCCTGTCCCTGTG 37 27 0 33.33 60.641 12.00 1.00 7.641 90 AATATTAATATTCCTGTCCCTGTGCC 40 26 0 38.46 61.650 12.00 1.00 7.650 91 ATTAATATTCCTGTCCCTGTGCCTC 43 25 0 44.00 62.718 6.00 1.00 7.718 92 CTCAAATATTAATATTCCTGTCCCTGT 36 27 0 33.33 59.222 12.00 0.00 7.778 93 TATTAATATTCCTGTCCCTGTGCCTC 42 26 0 42.31 62.643 8.00 1.00 8.643 94 CAAATATTAATATTCCTGTCCCTGTGC 38 27 0 37.04 62.049 12.00 2.00 9.049 95 AATATTAATATTCCTGTCCCTGTGCCT 40 27 0 37.04 62.311 12.00 0.00 9.311 96 AAATATTAATATTCCTGTCCCTGTGCC 39 27 0 37.04 62.579 12.00 1.00 9.579 97 ATATTAATATTCCTGTCCCTGTGCCTC 41 27 0 40.74 62.745 10.00 1.00 9.745 primer3-2.3.6/test/primer_list_last/AGAG.rev0000664000077300007730000001537112231156244017634 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TCTCTCTCTCTCTCTCTCTCTCTCTC 154 26 0 50.00 57.752 0.00 0.00 8.248 1 TCTCTCTCTCTCTCTCTCTCTCTCTC 152 26 0 50.00 57.752 0.00 0.00 8.248 2 TCTCTCTCTCTCTCTCTCTCTCTCTC 150 26 0 50.00 57.752 0.00 0.00 8.248 3 TCTCTCTCTCTCTCTCTCTCTCTCTC 148 26 0 50.00 57.752 0.00 0.00 8.248 4 TCTCTCTCTCTCTCTCTCTCTCTCTC 146 26 0 50.00 57.752 0.00 0.00 8.248 5 TCTCTCTCTCTCTCTCTCTCTCTCTC 144 26 0 50.00 57.752 0.00 0.00 8.248 6 TCTCTCTCTCTCTCTCTCTCTCTCTC 142 26 0 50.00 57.752 0.00 0.00 8.248 7 TCTCTCTCTCTCTCTCTCTCTCTCTC 140 26 0 50.00 57.752 0.00 0.00 8.248 8 TCTCTCTCTCTCTCTCTCTCTCTCTC 138 26 0 50.00 57.752 0.00 0.00 8.248 9 TCTCTCTCTCTCTCTCTCTCTCTCTC 136 26 0 50.00 57.752 0.00 0.00 8.248 10 TCTCTCTCTCTCTCTCTCTCTCTCTC 134 26 0 50.00 57.752 0.00 0.00 8.248 11 TCTCTCTCTCTCTCTCTCTCTCTCTC 132 26 0 50.00 57.752 0.00 0.00 8.248 12 TCTCTCTCTCTCTCTCTCTCTCTCTC 130 26 0 50.00 57.752 0.00 0.00 8.248 13 TCTCTCTCTCTCTCTCTCTCTCTCTC 128 26 0 50.00 57.752 0.00 0.00 8.248 14 TCTCTCTCTCTCTCTCTCTCTCTCTC 126 26 0 50.00 57.752 0.00 0.00 8.248 15 TCTCTCTCTCTCTCTCTCTCTCTCTC 124 26 0 50.00 57.752 0.00 0.00 8.248 16 TCTCTCTCTCTCTCTCTCTCTCTCTC 122 26 0 50.00 57.752 0.00 0.00 8.248 17 TCTCTCTCTCTCTCTCTCTCTCTCTC 120 26 0 50.00 57.752 0.00 0.00 8.248 18 TCTCTCTCTCTCTCTCTCTCTCTCTC 118 26 0 50.00 57.752 0.00 0.00 8.248 19 TCTCTCTCTCTCTCTCTCTCTCTCTC 116 26 0 50.00 57.752 0.00 0.00 8.248 20 TCTCTCTCTCTCTCTCTCTCTCTCTC 114 26 0 50.00 57.752 0.00 0.00 8.248 21 TCTCTCTCTCTCTCTCTCTCTCTCTC 112 26 0 50.00 57.752 0.00 0.00 8.248 22 TCTCTCTCTCTCTCTCTCTCTCTCTC 110 26 0 50.00 57.752 0.00 0.00 8.248 23 TCTCTCTCTCTCTCTCTCTCTCTCTC 108 26 0 50.00 57.752 0.00 0.00 8.248 24 TCTCTCTCTCTCTCTCTCTCTCTCTC 106 26 0 50.00 57.752 0.00 0.00 8.248 25 TCTCTCTCTCTCTCTCTCTCTCTCTC 104 26 0 50.00 57.752 0.00 0.00 8.248 26 TCTCTCTCTCTCTCTCTCTCTCTCTC 102 26 0 50.00 57.752 0.00 0.00 8.248 27 TCTCTCTCTCTCTCTCTCTCTCTCTC 100 26 0 50.00 57.752 0.00 0.00 8.248 28 TCTCTCTCTCTCTCTCTCTCTCTCTCT 154 27 0 48.15 58.701 0.00 0.00 8.299 29 CTCTCTCTCTCTCTCTCTCTCTCTCTC 153 27 0 51.85 58.701 0.00 0.00 8.299 30 TCTCTCTCTCTCTCTCTCTCTCTCTCT 152 27 0 48.15 58.701 0.00 0.00 8.299 31 CTCTCTCTCTCTCTCTCTCTCTCTCTC 151 27 0 51.85 58.701 0.00 0.00 8.299 32 TCTCTCTCTCTCTCTCTCTCTCTCTCT 150 27 0 48.15 58.701 0.00 0.00 8.299 33 CTCTCTCTCTCTCTCTCTCTCTCTCTC 149 27 0 51.85 58.701 0.00 0.00 8.299 34 TCTCTCTCTCTCTCTCTCTCTCTCTCT 148 27 0 48.15 58.701 0.00 0.00 8.299 35 CTCTCTCTCTCTCTCTCTCTCTCTCTC 147 27 0 51.85 58.701 0.00 0.00 8.299 36 TCTCTCTCTCTCTCTCTCTCTCTCTCT 146 27 0 48.15 58.701 0.00 0.00 8.299 37 CTCTCTCTCTCTCTCTCTCTCTCTCTC 145 27 0 51.85 58.701 0.00 0.00 8.299 38 TCTCTCTCTCTCTCTCTCTCTCTCTCT 144 27 0 48.15 58.701 0.00 0.00 8.299 39 CTCTCTCTCTCTCTCTCTCTCTCTCTC 143 27 0 51.85 58.701 0.00 0.00 8.299 40 TCTCTCTCTCTCTCTCTCTCTCTCTCT 142 27 0 48.15 58.701 0.00 0.00 8.299 41 CTCTCTCTCTCTCTCTCTCTCTCTCTC 141 27 0 51.85 58.701 0.00 0.00 8.299 42 TCTCTCTCTCTCTCTCTCTCTCTCTCT 140 27 0 48.15 58.701 0.00 0.00 8.299 43 CTCTCTCTCTCTCTCTCTCTCTCTCTC 139 27 0 51.85 58.701 0.00 0.00 8.299 44 TCTCTCTCTCTCTCTCTCTCTCTCTCT 138 27 0 48.15 58.701 0.00 0.00 8.299 45 CTCTCTCTCTCTCTCTCTCTCTCTCTC 137 27 0 51.85 58.701 0.00 0.00 8.299 46 TCTCTCTCTCTCTCTCTCTCTCTCTCT 136 27 0 48.15 58.701 0.00 0.00 8.299 47 CTCTCTCTCTCTCTCTCTCTCTCTCTC 135 27 0 51.85 58.701 0.00 0.00 8.299 48 TCTCTCTCTCTCTCTCTCTCTCTCTCT 134 27 0 48.15 58.701 0.00 0.00 8.299 49 CTCTCTCTCTCTCTCTCTCTCTCTCTC 133 27 0 51.85 58.701 0.00 0.00 8.299 50 TCTCTCTCTCTCTCTCTCTCTCTCTCT 132 27 0 48.15 58.701 0.00 0.00 8.299 51 CTCTCTCTCTCTCTCTCTCTCTCTCTC 131 27 0 51.85 58.701 0.00 0.00 8.299 52 TCTCTCTCTCTCTCTCTCTCTCTCTCT 130 27 0 48.15 58.701 0.00 0.00 8.299 53 CTCTCTCTCTCTCTCTCTCTCTCTCTC 129 27 0 51.85 58.701 0.00 0.00 8.299 54 TCTCTCTCTCTCTCTCTCTCTCTCTCT 128 27 0 48.15 58.701 0.00 0.00 8.299 55 CTCTCTCTCTCTCTCTCTCTCTCTCTC 127 27 0 51.85 58.701 0.00 0.00 8.299 56 TCTCTCTCTCTCTCTCTCTCTCTCTCT 126 27 0 48.15 58.701 0.00 0.00 8.299 57 CTCTCTCTCTCTCTCTCTCTCTCTCTC 125 27 0 51.85 58.701 0.00 0.00 8.299 58 TCTCTCTCTCTCTCTCTCTCTCTCTCT 124 27 0 48.15 58.701 0.00 0.00 8.299 59 CTCTCTCTCTCTCTCTCTCTCTCTCTC 123 27 0 51.85 58.701 0.00 0.00 8.299 60 TCTCTCTCTCTCTCTCTCTCTCTCTCT 122 27 0 48.15 58.701 0.00 0.00 8.299 61 CTCTCTCTCTCTCTCTCTCTCTCTCTC 121 27 0 51.85 58.701 0.00 0.00 8.299 62 TCTCTCTCTCTCTCTCTCTCTCTCTCT 120 27 0 48.15 58.701 0.00 0.00 8.299 63 CTCTCTCTCTCTCTCTCTCTCTCTCTC 119 27 0 51.85 58.701 0.00 0.00 8.299 64 TCTCTCTCTCTCTCTCTCTCTCTCTCT 118 27 0 48.15 58.701 0.00 0.00 8.299 65 CTCTCTCTCTCTCTCTCTCTCTCTCTC 117 27 0 51.85 58.701 0.00 0.00 8.299 66 TCTCTCTCTCTCTCTCTCTCTCTCTCT 116 27 0 48.15 58.701 0.00 0.00 8.299 67 CTCTCTCTCTCTCTCTCTCTCTCTCTC 115 27 0 51.85 58.701 0.00 0.00 8.299 68 TCTCTCTCTCTCTCTCTCTCTCTCTCT 114 27 0 48.15 58.701 0.00 0.00 8.299 69 CTCTCTCTCTCTCTCTCTCTCTCTCTC 113 27 0 51.85 58.701 0.00 0.00 8.299 70 TCTCTCTCTCTCTCTCTCTCTCTCTCT 112 27 0 48.15 58.701 0.00 0.00 8.299 71 CTCTCTCTCTCTCTCTCTCTCTCTCTC 111 27 0 51.85 58.701 0.00 0.00 8.299 72 TCTCTCTCTCTCTCTCTCTCTCTCTCT 110 27 0 48.15 58.701 0.00 0.00 8.299 73 CTCTCTCTCTCTCTCTCTCTCTCTCTC 109 27 0 51.85 58.701 0.00 0.00 8.299 74 TCTCTCTCTCTCTCTCTCTCTCTCTCT 108 27 0 48.15 58.701 0.00 0.00 8.299 75 CTCTCTCTCTCTCTCTCTCTCTCTCTC 107 27 0 51.85 58.701 0.00 0.00 8.299 76 TCTCTCTCTCTCTCTCTCTCTCTCTCT 106 27 0 48.15 58.701 0.00 0.00 8.299 77 CTCTCTCTCTCTCTCTCTCTCTCTCTC 105 27 0 51.85 58.701 0.00 0.00 8.299 78 TCTCTCTCTCTCTCTCTCTCTCTCTCT 104 27 0 48.15 58.701 0.00 0.00 8.299 79 CTCTCTCTCTCTCTCTCTCTCTCTCTC 103 27 0 51.85 58.701 0.00 0.00 8.299 80 TCTCTCTCTCTCTCTCTCTCTCTCTCT 102 27 0 48.15 58.701 0.00 0.00 8.299 81 CTCTCTCTCTCTCTCTCTCTCTCTCTC 101 27 0 51.85 58.701 0.00 0.00 8.299 82 TCTCTCTCTCTCTCTCTCTCTCTCTCT 100 27 0 48.15 58.701 0.00 0.00 8.299 83 CTCTCTCTCTCTCTCTCTCTCTCTCTC 99 27 0 51.85 58.701 0.00 0.00 8.299 primer3-2.3.6/test/primer_list_last/SP01-06-with-repeat3.int0000664000077300007730000003273312231156244022354 0ustar ioanaioanaACCEPTABLE INTERNAL OLIGOS 0-based # self self lib qual- # sequence start ln N GC% Tm any end sim lity 0 AAACGCAAAGCACGCTCC 113 18 0 55.56 62.465 4.00 0.00 8.00 4.465 1 AAAACGCAAAGCACGCTC 112 18 0 50.00 60.142 4.00 2.00 9.00 2.142 2 CAAAACGCAAAGCACGCTC 111 19 0 52.63 62.977 4.00 2.00 9.00 3.977 3 TCCAAAACGCAAAGCACG 109 18 0 50.00 61.944 2.00 2.00 9.00 3.944 4 TTCCAAAACGCAAAGCAC 108 18 0 44.44 58.389 2.00 0.00 9.00 3.611 5 CTTCCAAAACGCAAAGCAC 107 19 0 47.37 59.454 3.00 0.00 9.00 1.546 6 ACTTCCAAAACGCAAAGCAC 106 20 0 45.00 60.292 3.00 0.00 9.00 0.292 7 AACTTCCAAAACGCAAAGCAC 105 21 0 42.86 61.528 3.00 0.00 10.00 2.528 8 TAACTTCCAAAACGCAAAGCAC 104 22 0 40.91 61.484 3.00 0.00 10.00 3.484 9 CTAACTTCCAAAACGCAAAGCAC 103 23 0 43.48 62.256 3.00 0.00 10.00 5.256 10 CTTCCAAAACGCAAAGCA 107 18 0 44.44 58.546 3.00 0.00 9.00 3.454 11 ACTTCCAAAACGCAAAGCA 106 19 0 42.11 59.454 3.00 0.00 9.00 1.546 12 AACTTCCAAAACGCAAAGCA 105 20 0 40.00 60.782 3.00 0.00 10.00 0.782 13 TAACTTCCAAAACGCAAAGCA 104 21 0 38.10 60.764 3.00 0.00 10.00 1.764 14 CTAACTTCCAAAACGCAAAGCA 103 22 0 40.91 61.593 3.00 0.00 10.00 3.593 15 AACTTCCAAAACGCAAAGC 105 19 0 42.11 58.013 3.00 2.00 10.00 2.987 16 TAACTTCCAAAACGCAAAGC 104 20 0 40.00 58.097 3.00 2.00 10.00 1.903 17 CTAACTTCCAAAACGCAAAGC 103 21 0 42.86 59.065 3.00 2.00 10.00 1.935 18 TCTAACTTCCAAAACGCAAAGC 102 22 0 40.91 60.748 3.00 2.00 10.00 2.748 19 GTCTAACTTCCAAAACGCAAAGC 101 23 0 43.48 61.438 3.00 2.00 10.00 4.438 20 AGTCTAACTTCCAAAACGCAAAGC 100 24 0 41.67 62.180 4.00 2.00 10.00 6.180 21 TCTAACTTCCAAAACGCAAAG 102 21 0 38.10 57.272 3.00 0.00 10.00 3.728 22 GTCTAACTTCCAAAACGCAAAG 101 22 0 40.91 58.127 3.00 0.00 10.00 3.873 23 AGTCTAACTTCCAAAACGCAAAG 100 23 0 39.13 59.045 4.00 0.00 10.00 3.955 24 AAGTCTAACTTCCAAAACGCAAAG 99 24 0 37.50 60.206 6.00 0.00 10.00 4.206 25 AAAGTCTAACTTCCAAAACGCAAAG 98 25 0 36.00 61.265 6.00 0.00 10.00 6.265 26 GTCTAACTTCCAAAACGCAAA 101 21 0 38.10 57.124 3.00 0.00 10.00 3.876 27 AGTCTAACTTCCAAAACGCAAA 100 22 0 36.36 58.127 4.00 0.00 10.00 3.873 28 AAGTCTAACTTCCAAAACGCAAA 99 23 0 34.78 59.378 6.00 0.00 10.00 3.622 29 AAAGTCTAACTTCCAAAACGCAAA 98 24 0 33.33 60.517 6.00 0.00 10.00 4.517 30 CAAAGTCTAACTTCCAAAACGCAAA 97 25 0 36.00 62.766 6.00 0.00 10.00 7.766 31 AAGTCTAACTTCCAAAACGCAA 99 22 0 36.36 58.127 6.00 0.00 10.00 3.873 32 AAAGTCTAACTTCCAAAACGCAA 98 23 0 34.78 59.378 6.00 0.00 10.00 3.622 33 CAAAGTCTAACTTCCAAAACGCAA 97 24 0 37.50 61.761 6.00 0.00 10.00 5.761 34 AAAGTCTAACTTCCAAAACGCA 98 22 0 36.36 58.127 6.00 0.00 10.00 3.873 35 CAAAGTCTAACTTCCAAAACGCA 97 23 0 39.13 60.658 6.00 0.00 10.00 3.658 36 TCAAAGTCTAACTTCCAAAACGCA 96 24 0 37.50 62.242 6.00 0.00 10.00 6.242 37 GTCAAAGTCTAACTTCCAAAACGCA 95 25 0 40.00 62.860 6.00 0.00 10.00 7.860 38 CAAAGTCTAACTTCCAAAACGC 97 22 0 40.91 58.127 6.00 2.00 10.00 3.873 39 TCAAAGTCTAACTTCCAAAACGC 96 23 0 39.13 59.821 6.00 2.00 10.00 3.179 40 GTCAAAGTCTAACTTCCAAAACGC 95 24 0 41.67 60.537 6.00 2.00 10.00 4.537 41 TGTCAAAGTCTAACTTCCAAAACGC 94 25 0 40.00 62.860 6.00 2.00 10.00 7.860 42 GTCAAAGTCTAACTTCCAAAACG 95 23 0 39.13 57.204 6.00 2.00 10.00 5.796 43 TGTCAAAGTCTAACTTCCAAAACG 94 24 0 37.50 59.738 6.00 2.00 10.00 4.262 44 TTGTCAAAGTCTAACTTCCAAAACG 93 25 0 36.00 60.875 6.00 2.00 10.00 5.875 45 CTGTTGTCAAAGTCTAACTTCCAA 90 24 0 37.50 57.684 6.00 2.00 9.00 6.316 46 TCTGTTGTCAAAGTCTAACTTCCAA 89 25 0 36.00 59.394 6.00 2.00 9.00 5.606 47 GTCTGTTGTCAAAGTCTAACTTCCAA 88 26 0 38.46 60.127 6.00 2.00 9.00 6.127 48 AGTCTGTTGTCAAAGTCTAACTTCCAA 87 27 0 37.04 60.916 7.00 2.00 10.00 7.916 49 AGTCTGTTGTCAAAGTCTAACTTCC 87 25 0 40.00 57.318 7.00 2.00 10.00 7.682 50 GAGTCTGTTGTCAAAGTCTAACTTCC 86 26 0 42.31 58.988 7.00 2.00 10.00 7.012 51 TGAGTCTGTTGTCAAAGTCTAACTTCC 85 27 0 40.74 61.367 7.00 2.00 10.00 8.367 52 CAACTCACTGTATGAGTCTGTTGTC 73 25 0 44.00 57.993 6.00 1.00 11.00 7.007 53 CCAACTCACTGTATGAGTCTGTTGTC 72 26 0 46.15 61.357 6.00 2.00 11.00 7.357 54 AAACGCCAACTCACTGTATGA 67 21 0 42.86 58.296 3.00 2.00 10.00 2.704 55 GAAACGCCAACTCACTGTATGA 66 22 0 45.45 60.176 3.00 2.00 10.00 2.176 56 AGAAACGCCAACTCACTGTATGA 65 23 0 43.48 61.075 3.00 2.00 10.00 4.075 57 TAGAAACGCCAACTCACTGTATGA 64 24 0 41.67 61.047 3.00 2.00 10.00 5.047 58 ATAGAAACGCCAACTCACTGTATGA 63 25 0 40.00 61.238 3.00 2.00 10.00 6.238 59 GATAGAAACGCCAACTCACTGTATGA 62 26 0 42.31 62.768 3.00 2.00 10.00 8.768 60 GAAACGCCAACTCACTGTATG 66 21 0 47.62 58.296 3.00 1.00 10.00 2.704 61 AGAAACGCCAACTCACTGTATG 65 22 0 45.45 59.309 3.00 1.00 10.00 2.691 62 TAGAAACGCCAACTCACTGTATG 64 23 0 43.48 59.344 3.00 1.00 10.00 3.656 63 ATAGAAACGCCAACTCACTGTATG 63 24 0 41.67 59.623 3.00 1.00 10.00 4.377 64 GATAGAAACGCCAACTCACTGTATG 62 25 0 44.00 61.238 3.00 1.00 10.00 6.238 65 ATAGAAACGCCAACTCACTGTAT 63 23 0 39.13 57.058 3.00 2.00 10.00 5.942 66 GATAGAAACGCCAACTCACTGTAT 62 24 0 41.67 58.785 3.00 2.00 10.00 5.215 67 TGATAGAAACGCCAACTCACTGTAT 61 25 0 40.00 61.238 3.00 2.00 10.00 6.238 68 CTGATAGAAACGCCAACTCACTGTAT 60 26 0 42.31 61.990 3.00 2.00 10.00 7.990 69 GATAGAAACGCCAACTCACTGTA 62 23 0 43.48 58.464 3.00 2.00 10.00 4.536 70 TGATAGAAACGCCAACTCACTGTA 61 24 0 41.67 61.047 3.00 2.00 10.00 5.047 71 CTGATAGAAACGCCAACTCACTGTA 60 25 0 44.00 61.842 3.00 2.00 10.00 6.842 72 TGATAGAAACGCCAACTCACT 61 21 0 42.86 57.496 3.00 1.00 10.00 3.504 73 CTGATAGAAACGCCAACTCACT 60 22 0 45.45 58.536 3.00 1.00 10.00 3.464 74 CCTGATAGAAACGCCAACTCACT 59 23 0 47.83 61.863 3.00 1.00 10.00 4.863 75 ACCTGATAGAAACGCCAACTCACT 58 24 0 45.83 62.526 3.00 1.00 11.00 6.526 76 CTGATAGAAACGCCAACTCAC 60 21 0 47.62 57.496 3.00 0.00 10.00 3.504 77 CCTGATAGAAACGCCAACTCAC 59 22 0 50.00 61.033 3.00 0.00 10.00 3.033 78 ACCTGATAGAAACGCCAACTCAC 58 23 0 47.83 61.755 3.00 0.00 11.00 4.755 79 CCTGATAGAAACGCCAACTCA 59 21 0 47.62 60.256 3.00 1.00 10.00 1.256 80 ACCTGATAGAAACGCCAACTCA 58 22 0 45.45 61.033 3.00 1.00 11.00 3.033 81 GACCTGATAGAAACGCCAACTCA 57 23 0 47.83 62.713 3.00 1.00 11.00 5.713 82 CCTGATAGAAACGCCAACTC 59 20 0 50.00 57.422 2.00 0.00 10.00 2.578 83 ACCTGATAGAAACGCCAACTC 58 21 0 47.62 58.332 2.00 0.00 11.00 2.668 84 GACCTGATAGAAACGCCAACTC 57 22 0 50.00 60.137 2.00 0.00 11.00 2.137 85 TGACCTGATAGAAACGCCAACTC 56 23 0 47.83 62.713 2.00 0.00 11.00 5.713 86 ATGACCTGATAGAAACGCCAACTC 55 24 0 45.83 62.827 2.00 0.00 11.00 6.827 87 TATGACCTGATAGAAACGCCAACTC 54 25 0 44.00 62.743 4.00 0.00 11.00 7.743 88 GACCTGATAGAAACGCCAACT 57 21 0 47.62 58.332 2.00 1.00 11.00 2.668 89 TGACCTGATAGAAACGCCAACT 56 22 0 45.45 61.033 2.00 1.00 11.00 3.033 90 ATGACCTGATAGAAACGCCAACT 55 23 0 43.48 61.233 2.00 1.00 11.00 4.233 91 TATGACCTGATAGAAACGCCAACT 54 24 0 41.67 61.201 4.00 1.00 11.00 5.201 92 CTATGACCTGATAGAAACGCCAACT 53 25 0 44.00 61.959 6.00 1.00 11.00 6.959 93 GACCTGATAGAAACGCCAAC 57 20 0 50.00 57.265 2.00 0.00 11.00 2.735 94 TGACCTGATAGAAACGCCAAC 56 21 0 47.62 60.125 2.00 0.00 11.00 1.125 95 ATGACCTGATAGAAACGCCAAC 55 22 0 45.45 60.381 2.00 0.00 11.00 2.381 96 TATGACCTGATAGAAACGCCAAC 54 23 0 43.48 60.377 4.00 0.00 11.00 3.377 97 CTATGACCTGATAGAAACGCCAAC 53 24 0 45.83 61.201 6.00 0.00 11.00 5.201 98 TCTATGACCTGATAGAAACGCCAAC 52 25 0 44.00 62.743 8.00 0.00 11.00 7.743 99 TGACCTGATAGAAACGCCAA 56 20 0 45.00 59.272 2.00 0.00 11.00 0.728 100 ATGACCTGATAGAAACGCCAA 55 21 0 42.86 59.583 2.00 0.00 11.00 1.417 101 TATGACCTGATAGAAACGCCAA 54 22 0 40.91 59.609 4.00 0.00 11.00 2.391 102 CTATGACCTGATAGAAACGCCAA 53 23 0 43.48 60.495 6.00 0.00 11.00 3.495 103 TCTATGACCTGATAGAAACGCCAA 52 24 0 41.67 62.110 8.00 0.00 11.00 6.110 104 TGACCTGATAGAAACGCCA 56 19 0 47.37 57.759 2.00 0.00 11.00 3.241 105 ATGACCTGATAGAAACGCCA 55 20 0 45.00 58.173 2.00 0.00 11.00 1.827 106 TATGACCTGATAGAAACGCCA 54 21 0 42.86 58.254 4.00 0.00 11.00 2.746 107 CTATGACCTGATAGAAACGCCA 53 22 0 45.45 59.248 6.00 0.00 11.00 2.752 108 TCTATGACCTGATAGAAACGCCA 52 23 0 43.48 60.980 8.00 0.00 11.00 3.980 109 TTCTATGACCTGATAGAAACGCCA 51 24 0 41.67 62.110 10.00 0.00 11.00 6.110 110 TCTATGACCTGATAGAAACGCC 52 22 0 45.45 58.353 8.00 1.00 11.00 3.647 111 TTCTATGACCTGATAGAAACGCC 51 23 0 43.48 59.643 10.00 1.00 11.00 3.357 112 TTTCTATGACCTGATAGAAACGCC 50 24 0 41.67 60.811 12.00 1.00 11.00 4.811 113 TTTTCTATGACCTGATAGAAACGCC 49 25 0 40.00 61.874 12.00 2.00 11.00 6.874 114 TTTCTATGACCTGATAGAAACGC 50 23 0 39.13 57.615 12.00 2.00 11.00 5.385 115 CAATCAAATTTTTTCTATGACCTGAT 39 26 0 26.92 58.947 6.00 2.00 11.00 7.053 116 GCAATCAAATTTTTTCTATGACCTGAT 38 27 0 29.63 61.857 6.00 2.00 11.00 8.857 117 CAATCAAATTTTTTCTATGACCTGA 39 25 0 28.00 58.665 6.00 1.00 11.00 6.335 118 GCAATCAAATTTTTTCTATGACCTGA 38 26 0 30.77 61.716 6.00 1.00 11.00 7.716 119 CAATCAAATTTTTTCTATGACCTG 39 24 0 29.17 57.024 6.00 1.00 11.00 6.976 120 GCAATCAAATTTTTTCTATGACCTG 38 25 0 32.00 60.235 6.00 1.00 11.00 5.235 121 GCAATCAAATTTTTTCTATGACCT 38 24 0 29.17 57.882 6.00 1.00 11.00 6.118 122 CGCAATCAAATTTTTTCTATGACCT 37 25 0 32.00 61.717 6.00 1.00 11.00 6.717 123 CGCAATCAAATTTTTTCTATGACC 37 24 0 33.33 60.984 6.00 0.00 11.00 4.984 124 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 11.00 7.463 125 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 11.00 8.574 126 CGCAATCAAATTTTTTCTATGAC 37 23 0 30.43 57.905 6.00 1.00 10.00 5.095 127 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 10.00 4.458 128 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 10.00 5.211 129 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 10.00 6.843 130 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 10.00 9.235 131 CGCAATCAAATTTTTTCTATGA 37 22 0 27.27 57.077 6.00 2.00 10.00 4.923 132 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 10.00 4.207 133 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 10.00 5.202 134 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 10.00 7.653 135 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 10.00 4.923 136 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 10.00 5.544 137 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 10.00 5.323 138 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 10.00 5.202 139 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 11.00 8.397 140 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 12.00 9.507 141 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 10.00 5.439 142 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 11.00 4.023 143 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 12.00 5.202 144 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 12.00 6.204 145 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 12.00 8.199 146 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 10.00 4.533 147 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 11.00 3.033 148 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 12.00 4.199 149 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 12.00 5.202 150 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 12.00 7.226 151 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 12.00 8.382 152 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 11.00 2.909 153 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 12.00 3.626 154 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 12.00 4.596 155 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 12.00 5.502 156 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 12.00 6.694 157 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 12.00 8.652 primer3-2.3.6/test/primer_list_last/SP01-06.txt.rev0000664000077300007730000001703112231156244020654 0ustar ioanaioanaACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 GTGCTTTGCGTTTTGGAAGT 125 20 0 45.00 60.292 3.00 1.00 0.292 1 TGCTTTGCGTTTTGGAAGTT 124 20 0 40.00 60.782 3.00 3.00 0.782 2 GTGCTTTGCGTTTTGGAAG 125 19 0 47.37 59.454 3.00 0.00 1.546 3 TGCTTTGCGTTTTGGAAGT 124 19 0 42.11 59.454 3.00 1.00 1.546 4 TGCTTTGCGTTTTGGAAGTTA 124 21 0 38.10 60.764 3.00 2.00 1.764 5 GCTTTGCGTTTTGGAAGTTA 123 20 0 40.00 58.097 3.00 2.00 1.903 6 GCTTTGCGTTTTGGAAGTTAG 123 21 0 42.86 59.065 3.00 0.00 1.935 7 GAGCGTGCTTTGCGTTTT 129 18 0 50.00 60.142 4.00 0.00 2.142 8 GTGCTTTGCGTTTTGGAAGTT 125 21 0 42.86 61.528 3.00 3.00 2.528 9 GCTTTGCGTTTTGGAAGTTAGA 123 22 0 40.91 60.748 3.00 1.00 2.748 10 GCTTTGCGTTTTGGAAGTT 123 19 0 42.11 58.013 3.00 3.00 2.987 11 AGCGTGCTTTGCGTTTTG 128 18 0 50.00 61.131 4.00 0.00 3.131 12 GCGTTTTGGAAGTTAGACTTTGA 118 23 0 39.13 59.821 6.00 4.00 3.179 13 TGCTTTGCGTTTTGGAAG 124 18 0 44.44 58.546 3.00 0.00 3.454 14 GTGCTTTGCGTTTTGGAAGTTA 125 22 0 40.91 61.484 3.00 2.00 3.484 15 TGCTTTGCGTTTTGGAAGTTAG 124 22 0 40.91 61.593 3.00 0.00 3.593 16 GTGCTTTGCGTTTTGGAA 125 18 0 44.44 58.389 2.00 2.00 3.611 17 TTTGCGTTTTGGAAGTTAGACTT 121 23 0 34.78 59.378 6.00 6.00 3.622 18 TTGCGTTTTGGAAGTTAGACTTT 120 23 0 34.78 59.378 6.00 4.00 3.622 19 TGCGTTTTGGAAGTTAGACTTTG 119 23 0 39.13 60.658 6.00 2.00 3.658 20 CTTTGCGTTTTGGAAGTTAGA 122 21 0 38.10 57.272 3.00 1.00 3.728 21 CTTTGCGTTTTGGAAGTTAGAC 122 22 0 40.91 58.127 3.00 2.00 3.873 22 TTTGCGTTTTGGAAGTTAGACT 121 22 0 36.36 58.127 4.00 4.00 3.873 23 TTGCGTTTTGGAAGTTAGACTT 120 22 0 36.36 58.127 6.00 6.00 3.873 24 TGCGTTTTGGAAGTTAGACTTT 119 22 0 36.36 58.127 6.00 4.00 3.873 25 GCGTTTTGGAAGTTAGACTTTG 118 22 0 40.91 58.127 6.00 2.00 3.873 26 TTTGCGTTTTGGAAGTTAGAC 121 21 0 38.10 57.124 3.00 2.00 3.876 27 CGTGCTTTGCGTTTTGGA 126 18 0 50.00 61.944 2.00 0.00 3.944 28 CTTTGCGTTTTGGAAGTTAGACT 122 23 0 39.13 59.045 4.00 4.00 3.955 29 GAGCGTGCTTTGCGTTTTG 129 19 0 52.63 62.977 4.00 0.00 3.977 30 CTTTGCGTTTTGGAAGTTAGACTT 122 24 0 37.50 60.206 6.00 6.00 4.206 31 CGTTTTGGAAGTTAGACTTTGACA 117 24 0 37.50 59.738 6.00 4.00 4.262 32 GCTTTGCGTTTTGGAAGTTAGAC 123 23 0 43.48 61.438 3.00 2.00 4.438 33 GGAGCGTGCTTTGCGTTT 130 18 0 55.56 62.465 4.00 0.00 4.465 34 TTTGCGTTTTGGAAGTTAGACTTT 121 24 0 33.33 60.517 6.00 4.00 4.517 35 GCGTTTTGGAAGTTAGACTTTGAC 118 24 0 41.67 60.537 6.00 3.00 4.537 36 GATCGGGAGCGTGCTTTG 135 18 0 61.11 62.907 4.00 0.00 4.907 37 GTGCTTTGCGTTTTGGAAGTTAG 125 23 0 43.48 62.256 3.00 0.00 5.256 38 TTGGAAGTTAGACTTTGACAACAGA 113 25 0 36.00 59.394 6.00 3.00 5.606 39 TTTTGGAAGTTAGACTTTGACAACA 115 25 0 32.00 59.292 6.00 4.00 5.708 40 TTGCGTTTTGGAAGTTAGACTTTG 120 24 0 37.50 61.761 6.00 2.00 5.761 41 CGTTTTGGAAGTTAGACTTTGAC 117 23 0 39.13 57.204 6.00 3.00 5.796 42 CGTTTTGGAAGTTAGACTTTGACAA 117 25 0 36.00 60.875 6.00 6.00 5.875 43 TGGAAGTTAGACTTTGACAACAGA 112 24 0 37.50 58.112 6.00 3.00 5.888 44 TTTGGAAGTTAGACTTTGACAACA 114 24 0 33.33 58.041 6.00 3.00 5.959 45 GTTTTGGAAGTTAGACTTTGACAACA 116 26 0 34.62 60.009 8.00 5.00 6.009 46 TTTGGAAGTTAGACTTTGACAACAG 114 25 0 36.00 58.960 6.00 5.00 6.040 47 TGGAAGTTAGACTTTGACAACAGAC 112 25 0 40.00 58.927 6.00 5.00 6.073 48 TTTTGGAAGTTAGACTTTGACAACAG 115 26 0 34.62 60.120 6.00 5.00 6.120 49 TTGGAAGTTAGACTTTGACAACAGAC 113 26 0 38.46 60.127 6.00 5.00 6.127 50 GCTTTGCGTTTTGGAAGTTAGACT 123 24 0 41.67 62.180 4.00 4.00 6.180 51 TGGAAGTTAGACTTTGACAACAGACT 112 26 0 38.46 59.802 7.00 7.00 6.198 52 TGCGTTTTGGAAGTTAGACTTTGA 119 24 0 37.50 62.242 6.00 4.00 6.242 53 CTTTGCGTTTTGGAAGTTAGACTTT 122 25 0 36.00 61.265 6.00 4.00 6.265 54 TTGGAAGTTAGACTTTGACAACAG 113 24 0 37.50 57.684 6.00 5.00 6.316 55 CTTTGACAACAGACTCATACAGTGA 101 25 0 40.00 58.507 5.00 2.00 6.493 56 TTTGACAACAGACTCATACAGTGAG 100 25 0 40.00 58.507 5.00 4.00 6.493 57 TTTGACAACAGACTCATACAGTGA 100 24 0 37.50 57.479 5.00 2.00 6.521 58 CTTTGACAACAGACTCATACAGTGAG 101 26 0 42.31 59.446 5.00 4.00 6.554 59 TTTGGAAGTTAGACTTTGACAACAGA 114 26 0 34.62 60.557 6.00 3.00 6.557 60 GACTTTGACAACAGACTCATACAGTG 103 26 0 42.31 59.320 5.00 3.00 6.680 61 ACTTTGACAACAGACTCATACAGTGA 102 26 0 38.46 59.320 5.00 2.00 6.680 62 TTTGACAACAGACTCATACAGTGAGT 100 26 0 38.46 59.320 6.00 6.00 6.680 63 TTGACAACAGACTCATACAGTGAGTT 99 26 0 38.46 59.320 6.00 4.00 6.680 64 TTGACAACAGACTCATACAGTGAG 99 24 0 41.67 57.083 5.00 4.00 6.917 65 TTGACAACAGACTCATACAGTGAGT 99 25 0 40.00 57.993 6.00 6.00 7.007 66 GGAAGTTAGACTTTGACAACAGACTC 111 26 0 42.31 58.988 7.00 5.00 7.012 67 AGACTTTGACAACAGACTCATACAGTG 104 27 0 40.74 60.192 7.00 3.00 7.192 68 ACTTTGACAACAGACTCATACAGTGAG 102 27 0 40.74 60.192 5.00 4.00 7.192 69 CTTTGACAACAGACTCATACAGTGAGT 101 27 0 40.74 60.192 6.00 6.00 7.192 70 GTTTTGGAAGTTAGACTTTGACAAC 116 25 0 36.00 57.612 8.00 8.00 7.388 71 ACTTTGACAACAGACTCATACAGTG 102 25 0 40.00 57.555 5.00 3.00 7.445 72 TTTGACAACAGACTCATACAGTGAGTT 100 27 0 37.04 60.519 6.00 4.00 7.519 73 CGTTTTGGAAGTTAGACTTTGACAAC 117 26 0 38.46 61.521 8.00 8.00 7.521 74 GGAAGTTAGACTTTGACAACAGACT 111 25 0 40.00 57.318 7.00 7.00 7.682 75 GAAGTTAGACTTTGACAACAGACTCA 110 26 0 38.46 58.271 7.00 7.00 7.729 76 TTTGCGTTTTGGAAGTTAGACTTTG 121 25 0 36.00 62.766 6.00 2.00 7.766 77 GTTTTGGAAGTTAGACTTTGACAACAG 116 27 0 37.04 60.782 8.00 5.00 7.782 78 TGCGTTTTGGAAGTTAGACTTTGAC 119 25 0 40.00 62.860 6.00 3.00 7.860 79 GCGTTTTGGAAGTTAGACTTTGACA 118 25 0 40.00 62.860 6.00 4.00 7.860 80 TTGGAAGTTAGACTTTGACAACAGACT 113 27 0 37.04 60.916 7.00 7.00 7.916 81 GACTTTGACAACAGACTCATACAGTGA 103 27 0 40.74 60.988 5.00 2.00 7.988 82 TTTGGAAGTTAGACTTTGACAACAGAC 114 27 0 37.04 61.219 6.00 5.00 8.219 83 AGACTTTGACAACAGACTCATACAGT 104 26 0 38.46 57.680 7.00 3.00 8.320 84 TGGAAGTTAGACTTTGACAACAGACTC 112 27 0 40.74 61.367 7.00 5.00 8.367 85 GGAAGTTAGACTTTGACAACAGACTCA 111 27 0 40.74 61.367 7.00 7.00 8.367 86 GAAGTTAGACTTTGACAACAGACTCAT 110 27 0 37.04 58.586 7.00 5.00 8.414 87 TTTTGGAAGTTAGACTTTGACAACAGA 115 27 0 33.33 61.617 6.00 3.00 8.617 88 TTAGACTTTGACAACAGACTCATACA 106 26 0 34.62 57.298 7.00 1.00 8.702 89 TTAGACTTTGACAACAGACTCATACAG 106 27 0 37.04 58.234 7.00 1.00 8.766 90 TTGACAACAGACTCATACAGTGAGTTG 99 27 0 40.74 61.830 6.00 6.00 8.830 91 GTTAGACTTTGACAACAGACTCATACA 107 27 0 37.04 58.107 7.00 1.00 8.893 92 TAGACTTTGACAACAGACTCATACAGT 105 27 0 37.04 57.767 7.00 3.00 9.233 93 AAGTTAGACTTTGACAACAGACTCATA 109 27 0 33.33 57.047 7.00 3.00 9.953 primer3-2.3.6/test/primer_list_last/SP01-06.txt.for0000664000077300007730000000743012231156244020650 0ustar ioanaioanaACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 2.909 1 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 3.033 2 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 3.626 3 TGAATCGCAATCAAATTTTTT 32 21 0 23.81 57.334 6.00 4.00 3.666 4 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 4.023 5 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 4.199 6 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 4.207 7 ATGAATCGCAATCAAATTTTTT 31 22 0 22.73 57.710 6.00 4.00 4.290 8 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 4.458 9 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 4.533 10 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 4.596 11 ATCGCAATCAAATTTTTTCTATGA 35 24 0 25.00 59.083 6.00 3.00 4.917 12 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 4.923 13 TTATGAATCGCAATCAAATTTTTT 29 24 0 20.83 58.994 6.00 4.00 5.006 14 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 5.202 15 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 5.202 16 TGAATCGCAATCAAATTTTTTCTAT 32 25 0 24.00 60.202 6.00 4.00 5.202 17 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 5.202 18 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 5.202 19 TATGAATCGCAATCAAATTTTTT 30 23 0 21.74 57.795 6.00 4.00 5.205 20 TTATGAATCGCAATCAAATTTTT 29 23 0 21.74 57.795 6.00 4.00 5.205 21 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 5.211 22 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 5.323 23 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 5.439 24 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 5.502 25 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 5.544 26 ATTATGAATCGCAATCAAATTTTTT 28 25 0 20.00 59.255 6.00 4.00 5.745 27 AATTATGAATCGCAATCAAATTTTT 27 25 0 20.00 59.255 8.00 4.00 5.745 28 ATTATGAATCGCAATCAAATTTTT 28 24 0 20.83 58.122 6.00 4.00 5.878 29 AATTATGAATCGCAATCAAATTTT 27 24 0 20.83 58.122 8.00 5.00 5.878 30 GAATCGCAATCAAATTTTTTCTAT 33 24 0 25.00 57.900 6.00 4.00 6.100 31 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 6.204 32 ATGAATCGCAATCAAATTTTTTCTAT 31 26 0 23.08 60.408 6.00 4.00 6.408 33 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 6.694 34 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 6.843 35 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 7.226 36 TATGAATCGCAATCAAATTTTTTCTAT 30 27 0 22.22 60.404 6.00 4.00 7.404 37 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 7.463 38 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 7.653 39 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 8.199 40 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 8.382 41 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 8.397 42 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 8.574 43 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 8.652 44 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 9.235 45 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 9.507 primer3-2.3.6/test/primer_position_penalty_output0000664000077300007730000050433412231156245021367 0ustar ioanaioanaPRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=36-2000 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_GC_CLAMP=0 PRIMER_OPT_SIZE=20 PRIMER_MIN_SIZE=18 PRIMER_MAX_SIZE=27 PRIMER_OPT_TM=60.0 PRIMER_MIN_TM=57.0 PRIMER_MAX_TM=63.0 PRIMER_PAIR_MAX_DIFF_TM=100.0 PRIMER_MIN_GC=20.0 PRIMER_MAX_GC=80.0 PRIMER_SALT_MONOVALENT=50.0 PRIMER_DNA_CONC=50.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_SELF_ANY=8.00 PRIMER_MAX_SELF_END=3.00 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_MAX_POLY_X=5 PRIMER_LIBERAL_BASE=0 PRIMER_NUM_RETURN=1 PRIMER_FIRST_BASE_INDEX=0 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_MAX_LIBRARY_MISPRIMING=11.00 PRIMER_OUTSIDE_PENALTY=20.0 PRIMER_INSIDE_PENALTY=1000.0 SEQUENCE_ID=AT3_X1 SEQUENCE_TARGET=298,45 SEQUENCE_TEMPLATE=TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTCCCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACTTTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCTCTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCAGGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTCCCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAAGGACTCACAGGAATGACCTCCAACACCTTTGAGAAGACCAGGCCCTCTCCCTGGTAGTTACAGTCAAAGACCTGTTTGGAAGACGTCATTTCAAGTGCTCTCCCTCCCACCCCACCTCTTGGGGTAAGGCCTTTCCTAAGCTACCCCTTGGGTCCCTAGCCTAAGAAACAAGGGGGATGTCATCCCTGGTGTAAAGATGCTGTGCAGGAAGTCAGCACTCACGGGATCCAGGGGACGCTCCAAG PRIMER_LEFT_EXPLAIN=considered 3504, overlap target 289, GC content failed 16, low tm 818, high tm 1648, ok 733 PRIMER_RIGHT_EXPLAIN=considered 3504, overlap target 289, low tm 655, high tm 1723, ok 837 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.180022 PRIMER_LEFT_0_PENALTY=1.599465 PRIMER_RIGHT_0_PENALTY=1.580558 PRIMER_LEFT_0_SEQUENCE=CCCTGTGGAAGATTAGCGG PRIMER_RIGHT_0_SEQUENCE=GGGAAAGCTCACCCCTCTT PRIMER_LEFT_0=279,19 PRIMER_RIGHT_0=361,19 PRIMER_LEFT_0_TM=60.599 PRIMER_RIGHT_0_TM=60.581 PRIMER_LEFT_0_GC_PERCENT=57.895 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=7.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=11.4000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=83 = SEQUENCE_ID=AT3_X2 SEQUENCE_TARGET=298,371 SEQUENCE_TEMPLATE=AAACAGGAAGCTTCTCATTCTTTTTTCTCCCAGCGTGGGGAATTGAGCACATAATACTCCAAATAACCATCAGATGATTCTAATTCCAACATGACCACGTCCAGGCAACTGAACTGTCCCCTGGCAAGAAGTCTAGGACTGAACCTGTCCCGGGCCCCTGTACTTGGTTCAAAGGATTTAGCCTTTCTCTTGGCCACACCAGGTGGGCTGGAATCCTCTGCTTTACTGGGGCAACCCTGTGGTGGGCAGTGGGGCTAGGGGTTGCAGCCTAGCTTAACTTGGCATTTTGTCTCCTTGCAGGAAGGTTTATCTTTTGTCCTTGCTGCTCATTGGCTTCTGGGACTGCGTGACCTGTCACGGGAGCCCTGTGGACATCTGCACAGCCAAGCCGCGGGACATTCCCATGAATCCCATGTGCATTTACCGCTCCCCGGAGAAGAAGGCAACTGAGGATGAGGGCTCAGAACAGAAGATCCCGGAGGCCACCAACCGGCGTGTCTGGGAACTGTCCAAGGCCAATTCCCGCTTTGCTACCACTTTCTATCAGCACCTGGCAGATTCCAAGAATGACAATGATAACATTTTCCTGTCACCCCTGAGTATCTCCACGGCTTTTGCTATGACCAAGCTGGGTGCCTGTAATGACACCCTCCAGCAACTGATGGAGGTACGACCAAAGGTCTTCTGCCCAGCCACCTTGTTAGGAGCACCTTTGGGCTTCCATAGGCCCAAGTCCAATGATTCCTCAACCAACACTGCAGCCACTAGGGGCGCTCATTATGCATTACGATTCCCTTTGAACATCACTGTGTTATAATTCCCTTTGAAAATCATTTTTTAAAAAATTAGCCAAGGAATCTTGGCTATCTACTTTTTAAATCCTGGTTTCCTCTTTTGAGCACCTTAAAATGGGGGAAGGCTTGTATCTTCTCTCAACTTCTTTTCAGTAATTCTTTCATCTATATGT PRIMER_LEFT_EXPLAIN=considered 6612, overlap target 289, GC content failed 1, low tm 1035, high tm 3921, long poly-x seq 22, ok 1344 PRIMER_RIGHT_EXPLAIN=considered 6657, overlap target 289, GC content failed 114, low tm 1327, high tm 3439, high end compl 2, long poly-x seq 19, ok 1467 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=22.934112 PRIMER_LEFT_0_PENALTY=1.148919 PRIMER_RIGHT_0_PENALTY=21.785193 PRIMER_LEFT_0_SEQUENCE=TTGGCATTTTGTCTCCTTGC PRIMER_RIGHT_0_SEQUENCE=GGCAGAAGACCTTTGGTCG PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=688,19 PRIMER_LEFT_0_TM=61.149 PRIMER_RIGHT_0_TM=60.785 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MIR2 Repetitive element - a consensus PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=20.000000 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=9.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=411 = SEQUENCE_ID=AT3_X3 SEQUENCE_TARGET=298,220 SEQUENCE_TEMPLATE=CAAGAGGACTTTTCCCATTCCAGCAAGAAAACGTCTGTGTGTTGATCTAGAGGCGTTTAGAGACTTTAGGTGGCAACCTAGTCTCTCTTTTTCCCTTTATCCTTCCTACCCTTCATTCTTCTTTTATCCTTTTATTCATCAGAACACAAGAGTTGAGCATTTATGCTGTCCCAGGTACTGTGCTTGAAGGAGTTAACAACTGAGGTGGCTATTAGTCAGAGACTGACCAGCATGTGCTCACCACCCATGTTAACTAGGCAGCCCACCAAACCCACCACCATTTTTTTTTGACTTCTATAGGTATTTAAGTTTGACACCATATCTGAGAAAACATCTGATCAGATCCACTTCTTCTTTGCCAAACTGAACTGCCGACTCTATCGAAAAGCCAACAAATCCTCCAAGTTAGTATCAGCCAATCGCCTTTTTGGAGACAAATCCCTTACCTTCAATGAGACCTACCAGGACATCAGTGAGTTGGTATATGGAGCCAAGCTCCAGCCCCTGGACTTCAAGGTGAGTTGCAGATGTTACCCCTGACCTCCGAGTTCTTCCTCTCCACTCAGAGATTGAGGAGGTGGAGAAACAGCATCCAAATTCACACTGCTTTGCTGCTGAAGACTGCTGGAGGGCTGACTAAAAGTTAGAACCCCTGCAATAGTTATTCTTACTTGAAACCTGAGAAATCAAAGGTATCCATGCTTGGATTGTAGTGACTGCCCAGAAAACATGAATTAATAATCAATTCTTCATTCCATCCACCAACTTCAAATATATACCAAAGGGTGTTTTGAAGATGCCAGTTCTACAAGATAT PRIMER_LEFT_EXPLAIN=considered 5081, overlap target 289, GC content failed 13, low tm 1874, high tm 1352, long poly-x seq 25, ok 1528 PRIMER_RIGHT_EXPLAIN=considered 5254, overlap target 289, GC content failed 103, low tm 1706, high tm 1557, ok 1599 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=263.125741 PRIMER_LEFT_0_PENALTY=261.732281 PRIMER_RIGHT_0_PENALTY=1.393460 PRIMER_LEFT_0_SEQUENCE=CCAAACCCACCACCATTTTT PRIMER_RIGHT_0_SEQUENCE=CAGGGGTAACATCTGCAACTC PRIMER_LEFT_0=265,20 PRIMER_RIGHT_0=538,21 PRIMER_LEFT_0_TM=61.732 PRIMER_RIGHT_0_TM=59.607 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=260.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=274 = SEQUENCE_ID=AT3_X3' SEQUENCE_TARGET=298,142 SEQUENCE_TEMPLATE=AGTCAGAGAAAATAATATTTTATCTTCTTTTTCCCAGACTATTTTCGCCTTCCTTCTTTTCATTTATTTCTTCCTATTTCTTTTTGTCTTTTTCTTCTGATAATATTTATTAACTACAGGAAAGATTCATGGAACTATATTAGATATGTGAGGCTTCCCAATTTGGGTTAGAGCAATGGCTTCTTAATCAAATGGTGGGAAAGGACAGAGGGATGGTGAGAAAAATAAAATGCTGCCTGGGAAAATGGAGAAGCCAATTGAATAGCACAGGTGAGTAGGTTTATTTTCTGTTCTCCTCAGGAAAATGCAGAGCAATCCAGAGCGGCCATCAACAAATGGGTGTCCAATAAGACCGAAGGCCGAATCACCGATGTCATTCCCTCGGAAGCCATCAATGAGCTCACTGTTCTGGTGCTGGTTAACACCATTTACTTCAAGGTACTCAGAATGGCCCTGGAGAGACCCCAGGGACTTCCTCTTGCTCTTCAGCTTACCCCCTTTTTTTTTAAATGGCGAGACCGAAGCCCTGAGAGGGCAAATGGACTGCCGAAAGCTACACAGGTACAGGTCAGCAGGGCAGGTCAATCTATTATTTATTTATTTATTTATTTTTGACAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAAGCTTCGCCTCCTGGGTTCTCGGCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGAATACAGGC PRIMER_LEFT_EXPLAIN=considered 4474, overlap target 289, GC content failed 273, low tm 1464, high tm 1424, high repeat similarity 13, ok 1011 PRIMER_RIGHT_EXPLAIN=considered 4294, overlap target 289, GC content failed 209, low tm 512, high tm 2472, long poly-x seq 25, ok 787 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=128.448365 PRIMER_LEFT_0_PENALTY=127.241506 PRIMER_RIGHT_0_PENALTY=1.206859 PRIMER_LEFT_0_SEQUENCE=CACAGGTGAGTAGGTTTATTTTCTGTT PRIMER_RIGHT_0_SEQUENCE=CTCCAGGGCCATTCTGAGT PRIMER_LEFT_0=265,27 PRIMER_RIGHT_0=458,19 PRIMER_LEFT_0_TM=60.242 PRIMER_RIGHT_0_TM=60.207 PRIMER_LEFT_0_GC_PERCENT=37.037 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=120.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=194 = SEQUENCE_ID=AT3_X4 SEQUENCE_TARGET=298,395 SEQUENCE_TEMPLATE=GGCTTTGTCACTCATTTCTTCTTAGCATGGAATAGCATTACATTTGGTCTGGATGTACCACAGTCTGTCTATTCATCTACTGAAGGACATTTTGGCTGCTTCCAAGGTTTGACAGTTATGAATAAACCTACTCATAATTCCATCATTCTGACACAGCCATTGTTAACCTTTTTGTGCATATCCCGCCAGTCTTTTTTCCGAATAATTATATATTAATGTAACACTATAATATGGATATGTCTGTGTCAATAACTATCCTCCTATGAATGTTTGTGTTCTTACTTTGTGATTCTCTTCCAGGGCCTGTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGGAACTGTTCTACAAGGCTGATGGAGAGTCGTGTTCAGCATCTATGATGTACCAGGAAGGCAAGTTCCGTTATCGGCGCGTGGCTGAAGGCACCCAGGTGCTTGAGTTGCCCTTCAAAGGTGATGACATCACCATGGTCCTCATCTTGCCCAAGCCTGAGAAGAGCCTGGCCAAGGTGGAGAAGGAACTCACCCCAGAGGTGCTGCAGGAGTGGCTGGATGAATTGGAGGAGATGATGCTGGTGGTCCACATGCCCCGCTTCCGCATTGAGGACGGCTTCAGTTTGAAGGAGCAGCTGCAAGACATGGGCCTTGTCGATCTGTTCAGCCCTGAAAAGTCCAAACTCCCAGGTTTGTCTAGGAAGGAGTTTCCTCCCTTCTCCACCCGCAAGGTAGTCTGACCAAAAGTGGAAGAGTTGGAGAAAGAATAGAAAGGAGCAACAAGTCAGGACTCCTGGATACTGATCCTAGTTTCTACTGCTAATTTGTGGAAATCTCTTTTCCTTTTGAGACCTCAGTTTCCTCTTCTGTAAAAGGGAAGTTTGTTCTTGGATCTCCATGGGCCCAGCCAGCACTGGTGCCCTGTGAGTCTGTATCAGGTAGAGGAGATGGGACCAGGTGGAGAGGAATTTGAAAGGGCATTGGAATTCA PRIMER_LEFT_EXPLAIN=considered 6872, overlap target 289, GC content failed 226, low tm 1924, high tm 2932, long poly-x seq 17, ok 1484 PRIMER_RIGHT_EXPLAIN=considered 7004, overlap target 289, low tm 1493, high tm 3474, ok 1748 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=10.021418 PRIMER_LEFT_0_PENALTY=6.906112 PRIMER_RIGHT_0_PENALTY=3.115306 PRIMER_LEFT_0_SEQUENCE=TGTTCTTACTTTGTGATTCTCTTCC PRIMER_RIGHT_0_SEQUENCE=GGAGGAAACTCCTTCCTAGACAA PRIMER_LEFT_0=273,25 PRIMER_RIGHT_0=715,23 PRIMER_LEFT_0_TM=58.094 PRIMER_RIGHT_0_TM=60.115 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=47.826 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=443 = SEQUENCE_ID=AT3_X5 SEQUENCE_TARGET=298,69 SEQUENCE_TEMPLATE=TAGAGACAAGGTTTCACCATATTAGTTAGAGTGTCTCCAACTCCTGACCTCAGGTGATCTGTACACCTTGGCCTCCCGAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCCCCAAAGTACTTTATTATTTTTAACACATATTCATTGTGAGAGTATGATTAGGTGAAGATTTAGGATTTCTTCTTATGTTTCAAAAAGCCCCAAAGGATCTCTTAATCCAAACTGAATTCCCATCTGTGGATTGAAGCCAACTTTCTCCCATCTCACAAAGACTTCTCCGGTCTTCCTTCCAGGTATTGTTGCAGAAGGCCGAGATGACCTCTATGTCTCAGATGCATTCCATAAGGCATTTCTTGAGGTGAGTACACCTTCCCCACTCTCTTAGGGTACAGAAAGGAGATGCATGAACAGCAGGAACACGTGGAAAAGGCCTGTTTCCAGTGTTAAGGCATGCAAAAGGCCTCCACAGGCTGCTATAATACAGCCCTCTCCAAAACCTTCATGGTGTGATTGTTCTGCCTTCCCTCCCACTACCTCTTCTGTAGCAGGTCAAGCGGGAACACAAACATTTAGGGAGGGTGATATAGGAAAAGAAGCCAGCAAAGGCCATCAAGAAGAAATTTACAGCATGAGGAGAACCAGAAGAGTATGGGGTCGC PRIMER_LEFT_EXPLAIN=considered 3744, overlap target 289, GC content failed 148, low tm 1249, high tm 1208, ok 850 PRIMER_RIGHT_EXPLAIN=considered 3744, overlap target 289, low tm 874, high tm 1504, high end compl 8, ok 1069 PRIMER_PAIR_EXPLAIN=considered 21, high end compl 20, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=45.783665 PRIMER_LEFT_0_PENALTY=22.061137 PRIMER_RIGHT_0_PENALTY=23.722528 PRIMER_LEFT_0_SEQUENCE=AGACTTCTCCGGTCTTCCTTC PRIMER_RIGHT_0_SEQUENCE=AAGAGAGTGGGGAAGGTGTACT PRIMER_LEFT_0=276,21 PRIMER_RIGHT_0=389,22 PRIMER_LEFT_0_TM=58.939 PRIMER_RIGHT_0_TM=58.277 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=20.000000 PRIMER_RIGHT_0_POSITION_PENALTY=20.000000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=114 = SEQUENCE_ID=AT3_X6 SEQUENCE_TARGET=298,181 SEQUENCE_TEMPLATE=CCATTTACAATGGTACCAGCCAGAAATGTGATAACCTGTGTACATTCAGATTTCTGGGTTACCTGAATGGAACTCTTACACTTATTACCTAGCACAAGGCTTGGACAAACACAAGTACCTTACATTCTCTGCATGAAAGAATGAGTGAAAGTAGGATTCTGGAGGGAATCCAACCTGACCCAAATGTACTTTTTACTGGAAAACAAAAGCATTTGAGGAATTGCTGTGTCTGTGGATGATTTACCTGCCAAAATGAACGGCAGAGTGGCTAATTTAGTTTTATTCCCATGTGACCTGCAGGTAAATGAAGAAGGCAGTGAAGCAGCTGCAAGTACCGCTGTTGTGATTGCTGGCCGTTCGCTAAACCCCAACAGGGTGACTTTCAAGGCCAACAGGCCTTTCCTGGTTTTTATAAGAGAAGTTCCTCTGAACACTATTATCTTCATGGGCAGAGTAGCCAACCCTTGTGTTAAGTAAAATGTTCTTATTCTTTGCACCTCTTCCTATTTTTGGTTTGTGAACAGAAGTAAAAATAAATACAAACTACTTCCATCTCACATTATAAATGGACTCTGCATTTGAAATGAAGACAAGGAAAGGGGAAACATGCTATTGGGGCACATGGTAAAATGATGCCTTCAAGTTGTTCTTTACCCAGTAACCACATCTGGATCAAGAAAATGAGGGAGAGAGCGATAAAAGATGGTAGACAGCCAGAAAGGGAAGGGAGAG PRIMER_LEFT_EXPLAIN=considered 4819, overlap target 244, low tm 1696, high tm 1512, ok 1367 PRIMER_RIGHT_EXPLAIN=considered 4414, overlap target 289, GC content failed 103, low tm 1578, high tm 1288, high repeat similarity 14, ok 1142 PRIMER_PAIR_EXPLAIN=considered 3, high end compl 2, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=65.942667 PRIMER_LEFT_0_PENALTY=1.577239 PRIMER_RIGHT_0_PENALTY=64.365428 PRIMER_LEFT_0_SEQUENCE=TTTATTCCCATGTGACCTGC PRIMER_RIGHT_0_SEQUENCE=GGAAGAGGTGCAAAGAATAAGA PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=503,22 PRIMER_LEFT_0_TM=58.423 PRIMER_RIGHT_0_TM=57.635 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=60.000000 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=226 = SEQUENCE_ID=CETP_X1 SEQUENCE_TARGET=298,122 SEQUENCE_TEMPLATE=AGTAAAAATAAATGAATTCATGGAAACATATTAAGCAATTATCCAGATAACATAAGGGATGGCAAAAATGGTGCAGATGGTGGAGGGGAGACAAGTAGAAGTTGGGGTGCTCTTGTTGAATGTCTGGCTCTGAACTCTAGAGGAGGCCGCAGGGGCTGGGCAGGAAGGAGGTGAATCTCTGGGGCCAGGAAGACCCTGCTGCCCGGAAGAGCCTCATGTTCCGTGGGGGCTGGGCGGACATACATATACGGGCTCCAGGCTGAACGGCTCGGGCCACTTACACACCACTGCCTGATAACCATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAAGGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTGTAAGTATCAGTGCATCTGTCTGCCCTGCCAGGGGTCTTTTCATGGACACCCACTATGCCAGGAGCCTCCCTGGCCTGAAGCCAGCCCTGAAGCCGGCTGCCACACTAGCCCAGAGAGAGGAGTGCCCTGGGAGGGAGATGGGCTGAGTGGAGCTGTCATCACCCCCTCCTGACCTCGCCTTCAAGGTCAAGTTCTTTGGTGAGAAGGTCCTAGCTGCATTGCAAACAGCCAGGTATAGGGATTTGTGTTTGTCTGCGACCCAGAATCACTGGGGTTCGAGTTAGGGTTCAGATCTGAGC PRIMER_LEFT_EXPLAIN=considered 4274, overlap target 289, GC content failed 68, low tm 569, high tm 2779, ok 569 PRIMER_RIGHT_EXPLAIN=considered 4274, overlap target 289, low tm 375, high tm 2978, ok 632 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=7.862912 PRIMER_LEFT_0_PENALTY=4.158608 PRIMER_RIGHT_0_PENALTY=3.704304 PRIMER_LEFT_0_SEQUENCE=CTTACACACCACTGCCTGATAA PRIMER_RIGHT_0_SEQUENCE=GGCAGACAGATGCACTGATACTT PRIMER_LEFT_0=276,22 PRIMER_RIGHT_0=442,23 PRIMER_LEFT_0_TM=57.841 PRIMER_RIGHT_0_TM=60.704 PRIMER_LEFT_0_GC_PERCENT=45.455 PRIMER_RIGHT_0_GC_PERCENT=47.826 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=5.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=167 = SEQUENCE_ID=CETP_X2 SEQUENCE_TARGET=298,119 SEQUENCE_TEMPLATE=CTAGGGTTAGTGTGCGGCTGGGCTCAGGGCTGCCCCATTTGCTAGGATCGTGGGGTTCCCATGTGTCAGGATCCAGAGGCTAGGGTATGATCAGGATCTCTAGCTGGGGTCAGGGTCAGAGCTCTCTGTGTCCCCTAGAATTGCCATCAACCTTAAACCCAGAGGAGGCCCAGTCCAACCCCTCAGCTTTAAGACCTGGGAGCCTCATCTCAGAGAGGCTGAGTCATGGCCAAGGCAGTTGGGGTGGGAGCAGGGGGCTTGGTGTGGGCCTGCAGCCCTCATCCACTGCCCTCCCTCTAGTGAACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATATCACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAAGTGAGTCGGGCCTCGGGTGTGACCTGGCTGGGGGTAGGGTGGCGGGAGGAACAGCCTGGGCTTCCCCCAGCCACAGGGAGGAAAGGCAGCAGCTGGGGGACTCAGGTCTCTCCCCTTGATTTGGAACCAGAGCC PRIMER_LEFT_EXPLAIN=considered 4078, overlap target 123, GC content failed 7, low tm 374, high tm 2820, ok 754 PRIMER_RIGHT_EXPLAIN=considered 2584, overlap target 289, GC content failed 1, low tm 95, high tm 1901, ok 298 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=186.749017 PRIMER_LEFT_0_PENALTY=2.407131 PRIMER_RIGHT_0_PENALTY=184.341886 PRIMER_LEFT_0_SEQUENCE=ATCCACTGCCCTCCCTCT PRIMER_RIGHT_0_SEQUENCE=AGCCAGGTCACACCCGAG PRIMER_LEFT_0=280,18 PRIMER_RIGHT_0=443,18 PRIMER_LEFT_0_TM=59.593 PRIMER_RIGHT_0_TM=62.342 PRIMER_LEFT_0_GC_PERCENT=61.111 PRIMER_RIGHT_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_PAIR_0_LIBRARY_MISPRIMING=15.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=180.000000 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=9.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=164 = SEQUENCE_ID=CETP_X3 SEQUENCE_TARGET=298,139 SEQUENCE_TEMPLATE=TGCCTCCTCTTCCACTTTTCGGTACCCTGTGATTGATTGGGACCACCCAGATAACCTAGGATCATCTCCCCACCTACCCCAAGGTCCTTAACTTAACCATACTTCATATGGGTAACACGAGTTGAGTGTGGTACCCAGGTTTGACATGTTGGGTAACATATTTGCAGGTTCTGTGGATTAGGAGGACATTTTGGGGGCCATGATTCTATCTTCCACCCTCGCCTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGC PRIMER_LEFT_EXPLAIN=considered 4444, overlap target 289, low tm 844, high tm 2220, ok 1091 PRIMER_RIGHT_EXPLAIN=considered 4444, overlap target 289, low tm 400, high tm 2950, ok 805 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=2.234150 PRIMER_LEFT_0_PENALTY=0.821928 PRIMER_RIGHT_0_PENALTY=1.412222 PRIMER_LEFT_0_SEQUENCE=CCTCACTTCCATTCCTTCCC PRIMER_RIGHT_0_SEQUENCE=CATCAGCTGACAGGAATGCTT PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=457,21 PRIMER_LEFT_0_TM=60.822 PRIMER_RIGHT_0_TM=60.412 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=180 = SEQUENCE_ID=CETP_X4 SEQUENCE_TARGET=298,75 SEQUENCE_TEMPLATE=CTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCAT PRIMER_LEFT_EXPLAIN=considered 3804, overlap target 289, low tm 382, high tm 2360, ok 773 PRIMER_RIGHT_EXPLAIN=considered 3804, overlap target 289, low tm 348, high tm 2565, ok 602 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.692433 PRIMER_LEFT_0_PENALTY=2.413192 PRIMER_RIGHT_0_PENALTY=1.279241 PRIMER_LEFT_0_SEQUENCE=GGGTCCTTGGCTCTTTCC PRIMER_RIGHT_0_SEQUENCE=CCAGAGGACGCTTGACACAT PRIMER_LEFT_0=280,18 PRIMER_RIGHT_0=392,20 PRIMER_LEFT_0_TM=59.587 PRIMER_RIGHT_0_TM=61.279 PRIMER_LEFT_0_GC_PERCENT=61.111 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=113 = SEQUENCE_ID=CETP_X5 SEQUENCE_TARGET=298,92 SEQUENCE_TEMPLATE=GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCATCTCCAAGGGGAGCGAGAGTAAGTACACCACCCTGTGCCCCCATTCCTGTCGTGCCCATCCTGTTAGTGTGTCCACGGCCCCCTCCAGGCTCAACCCCACACAGGGATGCTTGTGGGTGGCCAAACCTGAGGGCAGCAATACCTTCAGTGGGGTCATTCCATCCCCCTCCATCAATACACCCTAAAGGCTGGAAACAACAATAACCAACAGCTAGTAACTAACAGCTATTAAGAACTTCTGTTGGCAAAGCACTATTCCAAGCCCTTTCATGAATTAATTGATTTTGTCCTTAAAACCAACCCTAGGATATAGATTCT PRIMER_LEFT_EXPLAIN=considered 3974, overlap target 289, low tm 348, high tm 2707, ok 630 PRIMER_RIGHT_EXPLAIN=considered 3974, overlap target 289, GC content failed 34, low tm 889, high tm 1971, ok 791 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=6.908902 PRIMER_LEFT_0_PENALTY=4.729227 PRIMER_RIGHT_0_PENALTY=2.179675 PRIMER_LEFT_0_SEQUENCE=CATGTCGGGTCTCCCTGC PRIMER_RIGHT_0_SEQUENCE=GGGCACAGGGTGGTGTACTT PRIMER_LEFT_0=280,18 PRIMER_RIGHT_0=409,20 PRIMER_LEFT_0_TM=62.729 PRIMER_RIGHT_0_TM=62.180 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=5.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=130 = SEQUENCE_ID=CETP_X6 SEQUENCE_TARGET=298,74 SEQUENCE_TEMPLATE=TTTCTGACTCTGGAGCAAGACGGATACATGTATGAATTTGGACTCTAGACACGTTCTCGTGTGTGTGACAGGTGTGAGCGTCACAGGAGCTGGGCCCTCCCGAGGAATTCTGGATGGTGCCACAGTTAATTCTTGGGTCTGAGGCTCCGTGTTCTCACTGCAAAATGGGAGTGATAATTCTTACTTCCTGAGCTACAAGAGTCAGGGCCAACAGAGCCATGAAGGAGCCTGGTACACACTAGGCGCTCCATGGATGCACAGGACTGGTCAGGGGCTCATTGTGGTGCTTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCAT PRIMER_LEFT_EXPLAIN=considered 3794, overlap target 289, low tm 681, high tm 1966, ok 858 PRIMER_RIGHT_EXPLAIN=considered 3794, overlap target 289, low tm 274, high tm 2616, high any compl 2, high end compl 3, ok 610 PRIMER_PAIR_EXPLAIN=considered 40, high end compl 38, ok 2 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=185.393869 PRIMER_LEFT_0_PENALTY=82.014189 PRIMER_RIGHT_0_PENALTY=103.379680 PRIMER_LEFT_0_SEQUENCE=CATTGTGGTGCTTGCTGC PRIMER_RIGHT_0_SEQUENCE=ACAGGGAGTCAGCCAGCCT PRIMER_LEFT_0=276,18 PRIMER_RIGHT_0=395,19 PRIMER_LEFT_0_TM=59.986 PRIMER_RIGHT_0_TM=62.380 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=80.000000 PRIMER_RIGHT_0_POSITION_PENALTY=100.000000 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=120 = SEQUENCE_ID=CETP_X7 SEQUENCE_TARGET=298,65 SEQUENCE_TEMPLATE=TTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCATGCCTCAGAAGACAGCAGTGGGAGCCAGAAAGCCACCTGCTGCACTATGTGGCCTTGGGACTGTCACTCTTCCTGTCTAGGTCCCATGGGCTCTATCTGGCTCTGACACTTGATGATTAGTTATGAGCATACTTTGGCAAAGCTCTGCCCCTTTGGTGCGGCTCACAAGCTGTGTGGCGAAGGGCTTGTCTATAGAACTCAGGACAAATGGGTGATTAAGTCCAAGAGGCATCCAAGATTCTCCTGGAAGTAGATTAGGAAAAAAGATAATTAGATTGC PRIMER_LEFT_EXPLAIN=considered 3704, overlap target 289, low tm 262, high tm 2572, high end compl 3, ok 578 PRIMER_RIGHT_EXPLAIN=considered 3593, overlap target 289, GC content failed 15, low tm 752, high tm 1654, long poly-x seq 22, ok 861 PRIMER_PAIR_EXPLAIN=considered 102, high any compl 81, high end compl 20, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=141.548415 PRIMER_LEFT_0_PENALTY=140.588967 PRIMER_RIGHT_0_PENALTY=0.959447 PRIMER_LEFT_0_SEQUENCE=CTGGAAAGCACCTGCTCTGT PRIMER_RIGHT_0_SEQUENCE=ATGCAGACAGAAACGCACTC PRIMER_LEFT_0=271,20 PRIMER_RIGHT_0=382,20 PRIMER_LEFT_0_TM=60.589 PRIMER_RIGHT_0_TM=59.041 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=140.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=112 = SEQUENCE_ID=CETP_X8 SEQUENCE_TARGET=298,96 SEQUENCE_TEMPLATE=GCTGGGCACTCATCCATGTACTGTACTCTCCTATGCAGTACAGAGCAGAGCTGGGTTTCAGCCCAAGTCTTGGACTCTGCTCTGAACCAACCTTCTAGAAGGGCTCTACCTACCCAGACAGACAGACTTGGGAAAAGAGAGAATGAAAAAGTGCCACACCCCTCCCCGCACACCCAGGTCCCACTTTACAGAGGGGAACACTGAGGCTGGAGGGTTGGGTAGCTGTGTGGATGCAGGGGACGGTGACTCAGGGCAATTCCCCCATCCCTGAGGCCCTGCGTTGATCTTTTCCTCCTGCAGCCAGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCATCACAGCCTCCTACCTGGAGTCCCATCACAAGGTAGGAGTTGTGGGAGGGTGGGCAGGGCCCAGCTTCCCCAGGGGAGTTGGTCCTTTTTTGTGCTCTGACAACCCCATCCCCCAGCTTCAACCTTATGGCAGCCAAGAGTCCTGGGGAGCTCCTCCTCATTCCTGATGCTCCTCCGCATTCCTGATGCTGCGAGGAGGGCAGGCCACAGCGACGTGCCCCTGACCCCTCTCTGCAGGCACCAGGGCTGCCCACTACAAGGATCCCAGCAAAGCACCAGCTCCTTCCTAGAGGGCTTATTCGGCTTCTGTCATCCTCTACAGCAGTGGATTG PRIMER_LEFT_EXPLAIN=considered 4014, overlap target 289, low tm 645, high tm 2223, ok 857 PRIMER_RIGHT_EXPLAIN=considered 3861, overlap target 289, GC content failed 8, low tm 269, high tm 2629, long poly-x seq 22, ok 644 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.514067 PRIMER_LEFT_0_PENALTY=0.186600 PRIMER_RIGHT_0_PENALTY=3.327467 PRIMER_LEFT_0_SEQUENCE=CGTTGATCTTTTCCTCCTGC PRIMER_RIGHT_0_SEQUENCE=CCACCCTCCCACAACTCCT PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=412,19 PRIMER_LEFT_0_TM=59.813 PRIMER_RIGHT_0_TM=62.327 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=1.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=135 = SEQUENCE_ID=CETP_X9 SEQUENCE_TARGET=163,184 SEQUENCE_TEMPLATE=TTTGTTGAATGAGTGAAAGCCGCTGGGGAAACTGGGTACAGCTCTTTCCTCAGTTTCCCCATCTGCACTCTGGGCTGAATGCTGGGCTCCTCCCAATCTCCCTGAAGCTGGACCTGAGCCCAGTAGGGACACACAGGGTCCAGCCAGCGTCCTGGCTTCCTCCAGGGTCATTTCATCTACAAGAATGTCTCAGAGGACCTCCCCCTCCCCACCTTCTCGCCCACACTGCTGGGGGACTCCCGCATGCTGTACTTCTGGTTCTCTGAGCGAGTCTTCCACTCGCTGGCCAAGGTAGCTTTCCAGGATGGCCGCCTCATGCTCAGCCTGATGGGAGACGAGTTCAAGGTGAGTGGGTGGGGCTGGGCTGCTAGGGGATCCAGATGGCATGTGGTATGTGTGTGTGTGCACACGCATGGGGAGGAGGGAGGAAACTCGGAAACTTGGTGGTGGGCAAAAGAACTAAGCTGGAGCAATAGCAGTGAAGTCCAGACTGGGCACAGTGGCTCACACCTGTAATCCCAATCCTTTGGGAGGCTGAGATGTAGCAGGACGAACCGCAGACAAAACTCCTCAGACACTGAGTTAAAGAAGGAAAGAGTTTATTCAGCCGGGAGCATGGGTAAGACTC PRIMER_LEFT_EXPLAIN=considered 3527, overlap target 272, low tm 323, high tm 2355, ok 577 PRIMER_RIGHT_EXPLAIN=considered 4589, overlap target 154, low tm 711, high tm 2838, ok 886 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=229.497858 PRIMER_LEFT_0_PENALTY=4.864632 PRIMER_RIGHT_0_PENALTY=224.633226 PRIMER_LEFT_0_SEQUENCE=AGCGTCCTGGCTTCCTCC PRIMER_RIGHT_0_SEQUENCE=ATCCCCTAGCAGCCCAGC PRIMER_LEFT_0=145,18 PRIMER_RIGHT_0=375,18 PRIMER_LEFT_0_TM=62.865 PRIMER_RIGHT_0_TM=62.633 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse MIR2 Repetitive element - a consensus PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=220.000000 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=231 = SEQUENCE_ID=CETP_X10 SEQUENCE_TARGET=235,55 SEQUENCE_TEMPLATE=CTTGTCGAGGTCACACAGCATGTGTGGGGCACAGTGGGGAGCAGAAGCCAGCCTCCAGCCGGACACCCCTTCCCCATTCCACACCCTGCCCAGAGCATCTCACATGTTGTCTGGGAGGTTGGGAGTTGCGTCTGAGGAGGGGTCCAGTCCTTGAAACTGCCCTTGGTCCCTGCGAAGTTTTCTTCTGAGGAGTGGACTTTACTCCACCCACCCTCCAACTTCCTCATTTCTTTTCAGGCAGTGCTGGAGACCTGGGGCTTCAACACCAACCAGGAAATCTTCCAAGAGGTAACTCCCCCCTGCCCCTGTGTGGGGTTTATCTCACGTACCCCAATCCTGCTCTGGCTTCAAGAGCCCCCACACAGCCAATAACACCACCAATGGCAACAATTATAACAG PRIMER_LEFT_EXPLAIN=considered 2785, overlap target 100, low tm 259, high tm 1906, ok 520 PRIMER_RIGHT_EXPLAIN=considered 1498, overlap target 226, low tm 153, high tm 792, long poly-x seq 22, ok 305 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=227.452455 PRIMER_LEFT_0_PENALTY=4.533349 PRIMER_RIGHT_0_PENALTY=222.919106 PRIMER_LEFT_0_SEQUENCE=TCCAACTTCCTCATTTCTTTTC PRIMER_RIGHT_0_SEQUENCE=ATAAACCCCACACAGGGGC PRIMER_LEFT_0=213,22 PRIMER_RIGHT_0=319,19 PRIMER_LEFT_0_TM=57.467 PRIMER_RIGHT_0_TM=61.919 PRIMER_LEFT_0_GC_PERCENT=36.364 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=220.000000 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=107 = SEQUENCE_ID=CETP_X11 SEQUENCE_TARGET=125,169 SEQUENCE_TEMPLATE=CCCCGAGCTACTTCCTTTTCCCAGCCCTGGGGCCCGGAGCCAGCTTTGTGGTTCCCATCTCCGAGGGCATGGACTGCTGCTCCGTGATGGGCCCCTGTCCTGGCCATGGGACCCCTGTCTTCCACAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTCCACTGCCTCAAGATGCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCAGTGATGGTGAAATTCCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTTGAAGAGGTGAGGCGGGTGCAGGGAGAGGTGGTGGTGGGGGAACTGACTCACATATGGGCCGCAGAGGGCAGGGGCTGGGGGTCTCTGAAGCCTCCAGATCCTTCTCACCACCTCTGCTGGCAC PRIMER_LEFT_EXPLAIN=considered 2831, overlap target 106, GC content failed 50, low tm 286, high tm 1982, ok 407 PRIMER_RIGHT_EXPLAIN=considered 2741, overlap target 116, GC content failed 42, low tm 336, high tm 1775, ok 472 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=125.433613 PRIMER_LEFT_0_PENALTY=2.311131 PRIMER_RIGHT_0_PENALTY=123.122481 PRIMER_LEFT_0_SEQUENCE=GGGACCCCTGTCTTCCAC PRIMER_RIGHT_0_SEQUENCE=ACCACCTCTCCCTGCACC PRIMER_LEFT_0=107,18 PRIMER_RIGHT_0=317,18 PRIMER_LEFT_0_TM=60.311 PRIMER_RIGHT_0_TM=61.122 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=120.000000 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=211 = SEQUENCE_ID=CETP_X12 SEQUENCE_TARGET=298,72 SEQUENCE_TEMPLATE=GTTGCAGTGAGCTGAGATCGTGCCACTGCCCTCCAGCCTGGGCAACAGAGTGAGACTGTCTCAAAAACAAAAAAAGAAAAGAAAAGAAAAAGAAAGTGACTTCTCAGGTCCTAACCCCAAAGCCACAGGTGCTGGGGAACTTTCCTCGGTTTTCAGAAGAGCAGTAGCTAAGCCTGGTTCCCGTGTCATCCTTGCCTCTCCAGTCCCTCAGTGGAAAGAATCAGGGGCCCTGAGCTAGGAGGGTTGCTCTCTGCTTCGGGAAGAGCCCTGGCTCACAGCAAATTTGGTTTCTCTCCCCAGGATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGCCTCTTGGATTTCCAGTATGTGCTGCAGAGAAGAGAAGGGGGCGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGT PRIMER_LEFT_EXPLAIN=considered 3686, overlap target 289, GC content failed 114, low tm 669, high tm 1809, long poly-x seq 14, ok 791 PRIMER_RIGHT_EXPLAIN=considered 3774, overlap target 289, low tm 619, high tm 2094, ok 772 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.608119 PRIMER_LEFT_0_PENALTY=1.522783 PRIMER_RIGHT_0_PENALTY=3.085335 PRIMER_LEFT_0_SEQUENCE=CAAATTTGGTTTCTCTCCCC PRIMER_RIGHT_0_SEQUENCE=CCTTCTCTTCTCTGCAGCACAT PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=391,22 PRIMER_LEFT_0_TM=58.477 PRIMER_RIGHT_0_TM=61.085 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=114 = SEQUENCE_ID=CETP_X13 SEQUENCE_TARGET=298,38 SEQUENCE_TEMPLATE=CGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGTCAAATTATCATCGCTTTTTTATTTCAGGATTACACCAAAGACTGTTTCCAACTTGACTGAGGTAGGTAGTCTTGGATAGACTGGGGGAAATAAGTCCTGTGGGACCTCCTGCCTTAAAGAAAGCAGGCGGAGGGCCCTAAAGGAAATCAGGCAACCAGACCAAAAGAATGTGACCAGGTGGTCCATGCTGTGTCTCTTGTGACCCTTCTTCTCCCTGCCATGTCTTTTGGGAGAGCCCTTGTGTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTC PRIMER_LEFT_EXPLAIN=considered 3282, overlap target 289, GC content failed 1, low tm 523, high tm 1815, long poly-x seq 22, ok 632 PRIMER_RIGHT_EXPLAIN=considered 3434, overlap target 289, low tm 586, high tm 1682, ok 877 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=126.624750 PRIMER_LEFT_0_PENALTY=123.526785 PRIMER_RIGHT_0_PENALTY=3.097964 PRIMER_LEFT_0_SEQUENCE=GGGTCAAATTATCATCGCTTTTT PRIMER_RIGHT_0_SEQUENCE=CCCCAGTCTATCCAAGACTACCT PRIMER_LEFT_0=269,23 PRIMER_RIGHT_0=358,23 PRIMER_LEFT_0_TM=60.527 PRIMER_RIGHT_0_TM=59.902 PRIMER_LEFT_0_GC_PERCENT=34.783 PRIMER_RIGHT_0_GC_PERCENT=52.174 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=120.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=90 = SEQUENCE_ID=CETP_X14 SEQUENCE_TARGET=298,77 SEQUENCE_TEMPLATE=GTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTCCCCAGGGCGAAGGAAAGACCACTCAGGAAGGGCACCGTCTGGGGCAGGAAAACGGAGTGGGTTGGATGTATTTTTTTCACGGATGGGCATGAGGATGAATGCTTGTCCAGGCCGTGCAGCATCTGCCTTGTGGGTCACTTCTGTGCTCCAGGGAGGACTCACCATGGGCATTTGATTGCAGAGCAGCTCCGAGTCCGTCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCTGAGGTCATGTCTCGTAAGTGTGGGCTGGAGGGGAAACTGGGTGCCGAGGCTGACAGAGCTTCCCATTTCACCTTTT PRIMER_LEFT_EXPLAIN=considered 3425, overlap target 52, GC content failed 3, low tm 235, high tm 2489, high end compl 2, long poly-x seq 23, ok 621 PRIMER_RIGHT_EXPLAIN=considered 1454, overlap target 289, low tm 83, high tm 882, ok 200 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=106.573018 PRIMER_LEFT_0_PENALTY=21.740693 PRIMER_RIGHT_0_PENALTY=84.832325 PRIMER_LEFT_0_SEQUENCE=CACCATGGGCATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GTTTCCCCTCCAGCCCAC PRIMER_LEFT_0=278,19 PRIMER_RIGHT_0=396,18 PRIMER_LEFT_0_TM=60.741 PRIMER_RIGHT_0_TM=62.832 PRIMER_LEFT_0_GC_PERCENT=47.368 PRIMER_RIGHT_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=20.000000 PRIMER_RIGHT_0_POSITION_PENALTY=80.000000 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=119 = SEQUENCE_ID=CETP_X15 SEQUENCE_TARGET=254,90 SEQUENCE_TEMPLATE=GGATGGGTTGGGAGCTCAAGTTTTGGGGCAGAAGGGAATTTTTTTTGGCAGCAGAGTGCAAGCCCTGCCGCCAGGCAAACTCTGCTCTTCCTCATCCTCAGAAGCACTTGCTCACTCTGCTAAATCAAAGTGAAACGCATGTTTACAGAATATTGGTCCAAAAGGGTCTCAGCATCTCCCACTACCCAGGGTGCAGAGCCTCGGGCCGGCCTTGCTCCCCAAGAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC PRIMER_LEFT_EXPLAIN=considered 3343, overlap target 289, GC content failed 24, low tm 488, high tm 1840, long poly-x seq 24, ok 678 PRIMER_RIGHT_EXPLAIN=considered 3758, overlap target 245, GC content failed 1, low tm 356, high tm 2464, high repeat similarity 3, long poly-x seq 22, ok 667 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=427.731805 PRIMER_LEFT_0_PENALTY=104.061036 PRIMER_RIGHT_0_PENALTY=323.670770 PRIMER_LEFT_0_SEQUENCE=ACTGGGGCTCTGTCCCCT PRIMER_RIGHT_0_SEQUENCE=AGGAACAGGGGCTGGTGA PRIMER_LEFT_0=231,18 PRIMER_RIGHT_0=377,18 PRIMER_LEFT_0_TM=62.061 PRIMER_RIGHT_0_TM=61.671 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=100.000000 PRIMER_RIGHT_0_POSITION_PENALTY=320.000000 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=147 = SEQUENCE_ID=CETP_X16 SEQUENCE_TARGET=298,79 SEQUENCE_TEMPLATE=GAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGCACCAGGCTCACAGCTGGAACCCTGGTGTCTCCTCCAGCGTGGTGGAAGTTGGGTTAGGAGTACGGAGATGGAGATTGGCTCCCAACTCCTCCCTATCCTAAAGGCCCACTGGCATTAAAGTGCTGTATCCAAGAGCTGCGGAGTCCTTCTTCTGTGGCTGGCGGGTAGAGGGGGGGGGAAGGGATTGTCTCACCAGTGCCGTCCACCTCTTTTCAGCCCTTCCAAGCAGCTGCCCCCAAACCCTCCAAGCTT PRIMER_LEFT_EXPLAIN=considered 3689, overlap target 286, GC content failed 3, low tm 288, high tm 2492, long poly-x seq 22, ok 598 PRIMER_RIGHT_EXPLAIN=considered 3637, overlap target 289, GC content failed 3, low tm 299, high tm 2316, long poly-x seq 25, ok 705 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.990886 PRIMER_LEFT_0_PENALTY=2.381034 PRIMER_RIGHT_0_PENALTY=2.609852 PRIMER_LEFT_0_SEQUENCE=CTCCTACTGCCCCTCCCTTC PRIMER_RIGHT_0_SEQUENCE=CCCGACCTCCTTGGAGAC PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=394,18 PRIMER_LEFT_0_TM=62.381 PRIMER_RIGHT_0_TM=60.610 PRIMER_LEFT_0_GC_PERCENT=65.000 PRIMER_RIGHT_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=117 = SEQUENCE_ID=F2_X1 SEQUENCE_TARGET=298,114 SEQUENCE_TEMPLATE=AAGCAACCACCGCTATATGTTAGGATTCGAAGGAGCTCCAGGAAAGTCTCATAGCCCCACTGGCCAGAATGGGCTAAATCTCAGAGGGGGAGGGTGGGAGATGGGGGTGACAGTGACCTTTTTTGTGACTCCTCCTAGACCATCCATCCCTGCTCCCAGGAGGACCTGTCCTCCCAGATGGTGGAGATGGACAGGAGGACTATCTACCCACCCGTCCCCACGGCCCTGACCCTCTGACCTCACCCTCTCCGCTGATTTCTTCATGTTAGTTCAACATTACCCAGAGGGGTCAGGACAGACAATTCCTCAGTGACCCAGGAGCTGACACACTATGGCGCACGTCCGAGGCTTGCAGCTGCCTGGCTGCCTGGCCCTGGCTGCCCTGTGTAGCCTTGTGCACAGCCAGCATGGTAAGGGAGTGCTTGCAGGCTGGAACAGGCTGGAGGACTGGGGTGTGGGCCCATGGGCTGGGGTCTCCTGGCTGGACAGAGCACACAGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGG PRIMER_LEFT_EXPLAIN=considered 4041, overlap target 289, GC content failed 21, low tm 498, high tm 2476, long poly-x seq 22, ok 735 PRIMER_RIGHT_EXPLAIN=considered 4194, overlap target 289, GC content failed 8, low tm 235, high tm 3082, high repeat similarity 2, ok 578 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=25.987217 PRIMER_LEFT_0_PENALTY=2.861995 PRIMER_RIGHT_0_PENALTY=23.125223 PRIMER_LEFT_0_SEQUENCE=CCAGAGGGGTCAGGACAG PRIMER_RIGHT_0_SEQUENCE=AGCCTGCAAGCACTCCCT PRIMER_LEFT_0=280,18 PRIMER_RIGHT_0=430,18 PRIMER_LEFT_0_TM=59.138 PRIMER_RIGHT_0_TM=61.125 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=21.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=20.000000 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=151 = SEQUENCE_ID=F2_X2 SEQUENCE_TARGET=298,165 SEQUENCE_TEMPLATE=AGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGGAGGGTGGGCTTGCTTCATGCCCCCAGATGGCCAAGACTGCCTGTTCCTGAGGTCGCTGTTCCATGACCCCCCCACCGCCTTTACAGTGTTCCTGGCTCCTCAGCAAGCACGGTCGCTGCTCCAGCGGGTCCGGCGAGCCAACACCTTCTTGGAGGAGGTGCGCAAGGGCAACCTAGAGCGAGAGTGCGTGGAGGAGACGTGCAGCTACGAGGAGGCCTTCGAGGCTCTGGAGTCCTCCACGGCTACGGTGAGCCTGGGCTGCTCGGACGGTGCCGGGGCCTCAGACCGGGCCCAACTCTAGACACTTCCACAGAGAAGCAAGCGAGGAACGCCACAGCCCCTTCGCTGCTCACAGCCTCATTTCAACTCTGAGCCCCTCCTCACAGGGCTGGCAAGAGGAGCGGCCTCAGCCTTTCCTGGGGGTCTCTGTGCCTGGACTGTGTCCCTGTGCAGCTCCATGACATGGGGAGGCCTCCACAGTCTTCAGACATCCACCTGCCTTGGAGCTCTGTGTCCACATGGCCTCCTCAGCGGCAGACTCCCACAC PRIMER_LEFT_EXPLAIN=considered 4542, overlap target 289, GC content failed 14, low tm 245, high tm 3332, long poly-x seq 23, ok 639 PRIMER_RIGHT_EXPLAIN=considered 4704, overlap target 289, GC content failed 96, low tm 191, high tm 3583, high end compl 4, ok 541 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=1004.486617 PRIMER_LEFT_0_PENALTY=302.054062 PRIMER_RIGHT_0_PENALTY=702.432556 PRIMER_LEFT_0_SEQUENCE=GTCGCTGTTCCATGACCC PRIMER_RIGHT_0_SEQUENCE=GTCTAGAGTTGGGCCCGGT PRIMER_LEFT_0=265,18 PRIMER_RIGHT_0=516,19 PRIMER_LEFT_0_TM=60.054 PRIMER_RIGHT_0_TM=61.433 PRIMER_LEFT_0_GC_PERCENT=61.111 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=7.00, reverse ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_0_LIBRARY_MISPRIMING=15.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=300.000000 PRIMER_RIGHT_0_POSITION_PENALTY=700.000000 PRIMER_LEFT_0_END_STABILITY=9.1000 PRIMER_RIGHT_0_END_STABILITY=11.1000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=252 = SEQUENCE_ID=F2_X3 SEQUENCE_TARGET=298,29 SEQUENCE_TEMPLATE=TGTTCCTGGAGCTCTGTGTCGCCTTTCCTGTCTGTAGGGACTCTGCCAGGGACCCACTGCCCCCTCTCCTCCCATCTCCCCCAGCCTCTTTCAGACTCGGTGTGTGTGTTGGAGGAACTCCCCTATCCTCAAATATTCTTCTCCTTTTGGAAACAAAAGTAGGAAACTCTGCCACAAACCTCCCCAGAGCCTGCCCCCTGCGTGACCAGGGTAAGGAAAGTGTGAGGAGGAGCATAACATTTACTAAAACAACACAAAACAGGAGCTGCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCA PRIMER_LEFT_EXPLAIN=considered 3344, overlap target 289, GC content failed 7, low tm 659, high tm 1679, ok 710 PRIMER_RIGHT_EXPLAIN=considered 3344, overlap target 289, GC content failed 1, low tm 379, high tm 2040, ok 635 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=2.486407 PRIMER_LEFT_0_PENALTY=0.475706 PRIMER_RIGHT_0_PENALTY=2.010701 PRIMER_LEFT_0_SEQUENCE=ACTCCCAGCCCTTGTTTTTC PRIMER_RIGHT_0_SEQUENCE=AAATCCTTCCCGGTGCTC PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=344,18 PRIMER_LEFT_0_TM=60.476 PRIMER_RIGHT_0_TM=60.011 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.556 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=67 = SEQUENCE_ID=F2_X4 SEQUENCE_TARGET=298,55 SEQUENCE_TEMPLATE=GCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCAACTGACACGGGTTTGGGGAGCAGGACATGGAGGGGAGCTTGGGAGAAGAGCTCAGGGGTGGGTTTGGAGTGTGGCTGGTGGAGGCCGAGGCAGTCCCCAGCATCTGACATTGCTCCCATTCCTGGGGTCAAGATGTCTCTTTGTACCTGGCTCTGTGTCTGGCATGCGAACGAATGAATGAATGAATGGACTAATGAATTAATGTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCACTGTAAACTCCGCCTCCCGGA PRIMER_LEFT_EXPLAIN=considered 3604, overlap target 289, GC content failed 1, low tm 409, high tm 2209, high any compl 3, high end compl 14, ok 679 PRIMER_RIGHT_EXPLAIN=considered 3497, overlap target 289, GC content failed 107, low tm 371, high tm 2132, long poly-x seq 18, ok 580 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=123.319487 PRIMER_LEFT_0_PENALTY=121.626330 PRIMER_RIGHT_0_PENALTY=1.693157 PRIMER_LEFT_0_SEQUENCE=CCAAAGGTAAACACCTGGGTC PRIMER_RIGHT_0_SEQUENCE=CAAACCCGTGTCAGTTGCTC PRIMER_LEFT_0=271,21 PRIMER_RIGHT_0=372,20 PRIMER_LEFT_0_TM=60.626 PRIMER_RIGHT_0_TM=61.693 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=15.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=120.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=9.1000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=102 = SEQUENCE_ID=F2_X5 SEQUENCE_TARGET=298,110 SEQUENCE_TEMPLATE=TGCTGGCCAAGAGAAGCCACAGAATGGAAGCTCCATGAGGGCAGGGCTGTGACTGTCCTATTGGTTGATGTGTACTGAGCACCCGACAGTGCCTGTCATATGGTAGGCACTTAGCGAATATTTGGAGGCCACTGTTGAGTGAATGGGAGAACTGCTGGTTGCAGAGGAAGAGGGGCTGGGTGAATGCAGGTTCAGGATTGTGGACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGG PRIMER_LEFT_EXPLAIN=considered 4154, overlap target 289, GC content failed 2, low tm 468, high tm 2494, ok 901 PRIMER_RIGHT_EXPLAIN=considered 4154, overlap target 289, GC content failed 14, low tm 310, high tm 2916, ok 625 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=328.554484 PRIMER_LEFT_0_PENALTY=323.965248 PRIMER_RIGHT_0_PENALTY=4.589237 PRIMER_LEFT_0_SEQUENCE=CAGGCTCCAAGGCTGACC PRIMER_RIGHT_0_SEQUENCE=AAGGCCGACCCCTCACTC PRIMER_LEFT_0=264,18 PRIMER_RIGHT_0=425,18 PRIMER_LEFT_0_TM=61.965 PRIMER_RIGHT_0_TM=62.589 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=320.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=162 = SEQUENCE_ID=F2_X6 SEQUENCE_TARGET=298,141 SEQUENCE_TEMPLATE=GACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGGATGGGAACCAAGAATACTGGCTACCCAGGCACAGTGGCTCATGCCCGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAGGTCAGGGGTTTGAGACCAGCTGGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAATTGCCAGGCGTGGTGGTGGGCGCCTGTAATCCCAACTACTCTGGAGGCTGAGGCACGAGAATCGCTTGAACCCGGGAG PRIMER_LEFT_EXPLAIN=considered 4464, overlap target 289, GC content failed 2, low tm 392, high tm 2999, ok 782 PRIMER_RIGHT_EXPLAIN=considered 4464, overlap target 289, GC content failed 40, low tm 325, high tm 3245, ok 565 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=1184.087291 PRIMER_LEFT_0_PENALTY=0.806671 PRIMER_RIGHT_0_PENALTY=1183.280619 PRIMER_LEFT_0_SEQUENCE=GTTGGGCAATTTCCTCTTCC PRIMER_RIGHT_0_SEQUENCE=CTTGGTTCCCATCCCAGG PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=515,18 PRIMER_LEFT_0_TM=60.807 PRIMER_RIGHT_0_TM=61.281 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=1180.000000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=238 = SEQUENCE_ID=F2_X7 SEQUENCE_TARGET=298,319 SEQUENCE_TEMPLATE=GCAGCTCCAGGCAGCCACTACCAGTTGGTTACAGATGGCCTAGGAGGCCAAACCTGGTTACTATCTCTGGTTTATTATGTGCCAGACACTTATGCTGTATATTTTGTTTAATCCTCTCAACAAACCTGCAAAAGTGGCATTAGTAACCCCTTTAAAGGCAAACGGTCAGAAGCCCAGAGAGGTTAAGTAACCTGAGGTCACACAGGCAGAAAGCAGCAAGACCGGGGTTCACACCCCTGTCTGTTCCGGTCCATGTGTGGTCTCACTCACTCTGCTGCCTCCTTGCCCCTCACCCACCAGGCCAGGATCAAGTCACTGTAGCGATGACTCCACGCTCCGAAGGCTCCAGTGTGAATCTGTCACCTCCATTGGAGCAGTGTGTCCCTGATCGGGGGCAGCAGTACCAGGGGCGCCTGGCGGTGACCACACATGGGCTCCCCTGCCTGGCCTGGGCCAGCGCACAGGCCAAGGCCCTGAGCAAGCACCAGGACTTCAACTCAGCTGTGCAGCTGGTGGAGAACTTCTGCCGCAACCCAGACGGGGATGAGGAGGGCGTGTGGTGCTATGTGGCCGGGAAGCCTGGCGACTTTGGGTACTGCGACCTCAACTATTGTGGTGAGCTGCCTGGGTAGGGGGCCTGAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCC PRIMER_LEFT_EXPLAIN=considered 6244, overlap target 289, GC content failed 60, low tm 973, high tm 3795, ok 1127 PRIMER_RIGHT_EXPLAIN=considered 5938, overlap target 289, GC content failed 51, low tm 580, high tm 3983, long poly-x seq 44, ok 991 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=369.200187 PRIMER_LEFT_0_PENALTY=224.254433 PRIMER_RIGHT_0_PENALTY=144.945754 PRIMER_LEFT_0_SEQUENCE=CTCTGCTGCCTCCTTGCC PRIMER_RIGHT_0_SEQUENCE=CTCAGGCCCCCTACCCAG PRIMER_LEFT_0=269,18 PRIMER_RIGHT_0=641,18 PRIMER_LEFT_0_TM=62.254 PRIMER_RIGHT_0_TM=62.946 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=72.222 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=220.000000 PRIMER_RIGHT_0_POSITION_PENALTY=140.000000 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=373 = SEQUENCE_ID=F2_X8 SEQUENCE_TARGET=298,133 SEQUENCE_TEMPLATE=GAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGG PRIMER_LEFT_EXPLAIN=considered 4078, overlap target 289, GC content failed 87, low tm 484, high tm 2399, high end compl 1, long poly-x seq 44, ok 774 PRIMER_RIGHT_EXPLAIN=considered 4384, overlap target 289, GC content failed 97, low tm 244, high tm 3201, ok 553 PRIMER_PAIR_EXPLAIN=considered 14, high end compl 12, ok 2 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=706.658308 PRIMER_LEFT_0_PENALTY=683.278099 PRIMER_RIGHT_0_PENALTY=23.380210 PRIMER_LEFT_0_SEQUENCE=GGATGGGTGAGGAATGGC PRIMER_RIGHT_0_SEQUENCE=CCTCGGATGCCCACTACCT PRIMER_LEFT_0=246,18 PRIMER_RIGHT_0=450,19 PRIMER_LEFT_0_TM=61.278 PRIMER_RIGHT_0_TM=62.380 PRIMER_LEFT_0_GC_PERCENT=61.111 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=680.000000 PRIMER_RIGHT_0_POSITION_PENALTY=20.000000 PRIMER_LEFT_0_END_STABILITY=9.6000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=205 = SEQUENCE_ID=F2_X9 SEQUENCE_TARGET=298,131 SEQUENCE_TEMPLATE=CCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGGCTTTACAGATGACAACAGCTGAGCATCCAGGATCCCACCAACTCCACACAGCAGCCACATGAGATGGGTTGTTTACTTCTTTTTTTTTTGTTTCTTAGATGGAGTCTTGCTCTGTCACCTAGGCTGGAGTGCAGTGCTGCAATCTCGGCTCACTACCTCGATCTCAGCTCACTGCAACTTCTGCCTTCCGGGTTCAAACGATTCTCTTGCC PRIMER_LEFT_EXPLAIN=considered 4212, overlap target 289, GC content failed 182, low tm 233, high tm 2939, long poly-x seq 22, ok 547 PRIMER_RIGHT_EXPLAIN=considered 4248, overlap target 289, GC content failed 80, low tm 415, high tm 2693, long poly-x seq 23, ok 748 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=166.167609 PRIMER_LEFT_0_PENALTY=2.845950 PRIMER_RIGHT_0_PENALTY=163.321659 PRIMER_LEFT_0_SEQUENCE=ACTGCTTGGCTTGCTCTGC PRIMER_RIGHT_0_SEQUENCE=AATGGTAGCGCAGGGCTC PRIMER_LEFT_0=279,19 PRIMER_RIGHT_0=454,18 PRIMER_LEFT_0_TM=61.846 PRIMER_RIGHT_0_TM=61.322 PRIMER_LEFT_0_GC_PERCENT=57.895 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=160.000000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=176 = SEQUENCE_ID=F2_X10 SEQUENCE_TARGET=298,172 SEQUENCE_TEMPLATE=CCTGAGCCCAAGAGGCTGAGGCTTCAGTAAGCTGTGACTGTACCATTGCACTCCAGCCTGGGTGACAAGAGTGAGACCCTGTCTCAAAAATAAATAAATAAATAAATAAAAAGTGTGAGGCAGCCCCTCAGCATCACACGGAGGCTCCAGCCCCAAAGGCGGCCAGCCCAAGCTTGGATCTGGGCCCCGGAGGCAGCTCTGCCCAGCTGGGTTCTTAGACCTGGGATTGTTACTTCTAGGGCTGGTGTAGAGGCAGCCCCCTCATCCTCAGCTCCTAATGCTTCCTGCTGCCCCTCCCAGGCAGGTGATGCTTTTCCGGAAGAGTCCCCAGGAGCTGCTGTGTGGGGCCAGCCTCATCAGTGACCGCTGGGTCCTCACCGCCGCCCACTGCCTCCTGTACCCGCCCTGGGACAAGAACTTCACCGAGAATGACCTTCTGGTGCGCATTGGCAAGCACTCCCGCACAAGGTACAGAACTGGTGGCCCGTGGGTGTCTGGCAGGGGTCTGAGTCCTCCAAAGCGATCATGAGGGGCCTTGGTGGCTCCGGGACACATAGGATGTTCTGTATACCCCCCAGAATATAACATCCCAGCAGTCTCTGCTGGAAAGCCATTTGGTCACGTCCTGACTGAGGCTTGGAGCGCGGGGAGAATCCGTCTGTCTCTGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGC PRIMER_LEFT_EXPLAIN=considered 4774, overlap target 289, GC content failed 210, low tm 485, high tm 3153, ok 637 PRIMER_RIGHT_EXPLAIN=considered 4621, overlap target 289, GC content failed 8, low tm 474, high tm 3110, long poly-x seq 22, ok 718 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=105.472156 PRIMER_LEFT_0_PENALTY=101.889081 PRIMER_RIGHT_0_PENALTY=3.583075 PRIMER_LEFT_0_SEQUENCE=CTAATGCTTCCTGCTGCCC PRIMER_RIGHT_0_SEQUENCE=ACGGGCCACCAGTTCTGT PRIMER_LEFT_0=274,19 PRIMER_RIGHT_0=487,18 PRIMER_LEFT_0_TM=60.889 PRIMER_RIGHT_0_TM=61.583 PRIMER_LEFT_0_GC_PERCENT=57.895 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=100.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=11.2000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=214 = SEQUENCE_ID=F2_X11 SEQUENCE_TARGET=298,178 SEQUENCE_TEMPLATE=TGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGCTGTCTGACTCCAAAGCCCTGCACGGCTTTAGGCCCAGGAAGAAACACCCAGGGGGCTGCCATGGCAGGAACCAGCCCTATCCCCTCCCTGGTGGCCTGCAGGACACACTGTCTCCCAGAACCCCAAGGGCAGGCAGTTTCCTGCTCCTTGCTGGGTGAACCTGCAGCTTCTCCATTTCTTTCTTGGGGTCTCTGCAGGTACGAGCGAAACATTGAAAAGATATCCATGTTGGAAAAGATCTACATCCACCCCAGGTACAACTGGCGGGAGAACCTGGACCGGGACATTGCCCTGATGAAGCTGAAGAAGCCTGTTGCCTTCAGTGACTACATTCACCCTGTGTGTCTGCCCGACAGGGAGACGGCAGCCAGGTGGGCCACCAGATGCTTGTTAGCTGAGGGGCAGAAGCCAAGTTCTGGGCCTGGCTCTGATACCAAGTAGCCTTGCAAGAGCCCCTTTCCCTTTTCCAGGCCTCGGTTTCTTGGAGTGAACCCAAAAGTTCTTTTCAGTACTGGCGTTTTATTTTTTATTTATATTTATTTATTTACTGACGGAGTTCCACTCTTGTCTCCCAGGCTGGAGTGTAGTTGTGCGATCTTGGCTCACTGCAACCCCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACA PRIMER_LEFT_EXPLAIN=considered 4834, overlap target 289, low tm 620, high tm 3010, ok 915 PRIMER_RIGHT_EXPLAIN=considered 4762, overlap target 289, GC content failed 176, low tm 739, high tm 2528, long poly-x seq 13, ok 1017 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.703189 PRIMER_LEFT_0_PENALTY=0.594713 PRIMER_RIGHT_0_PENALTY=4.108476 PRIMER_LEFT_0_SEQUENCE=TTCTTTCTTGGGGTCTCTGC PRIMER_RIGHT_0_SEQUENCE=ACAAGCATCTGGTGGCCC PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=493,18 PRIMER_LEFT_0_TM=59.405 PRIMER_RIGHT_0_TM=62.108 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=216 = SEQUENCE_ID=F2_X12 SEQUENCE_TARGET=298,186 SEQUENCE_TEMPLATE=CCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACAGGCTAATTTTTGTATTTTTAGTAGAGACTGGTGGGTTTCACCGTGTCGGCCAGGTTGGTCTCGAACCCCTGACCTCAAGTGATTCACCCGCCTCGGCCTCCCAAAGTGCCGAGACCACAGGCGTGAACGTCTGTGCCCAGCCAGCTCTGGCGTTTTAGATTCTGGTCTCTAAGAAATGGCGTTGGGGCCAGGCGGCTCCTGTGGGGGTTGGCTCTCACTAGGCCCTTCTTCCTTCCCCAAAGCTTGCTCCAGGCTGGATACAAGGGGCGGGTGACAGGCTGGGGCAACCTGAAGGAGACGTGGACAGCCAACGTTGGTAAGGGGCAGCCCAGTGTCCTGCAGGTGGTGAACCTGCCCATTGTGGAGCGGCCGGTCTGCAAGGACTCCACCCGGATCCGCATCACTGACAACATGTTCTGTGCTGGCAAGTCTGTGCAGGGCGGGCTGAGGGAACAGTGGGGCCCAAGCTGGGAGAACTGAGTTGTGCCTGGGTTCAAGCCATGTGACTTTGAGCAAGTTGCCTAACCTCTTGGTGGCTCAGTTTCTTCCTCTGTAAAATGGAGGTAAAAGTCTCTATCCCATAAGGTTATGGGAGGGTTAAATGAAGTAGTATATATTAATGTACTTGGCATAGTATCAGTCACCAGTGAGCTCAGATAGCAGCAAGAGGCTGCGGGTAGGGAAATGCCATTCATTCAGTCACTCAGCAAATATTTATTGAGCG PRIMER_LEFT_EXPLAIN=considered 4914, overlap target 289, GC content failed 56, low tm 524, high tm 3341, ok 704 PRIMER_RIGHT_EXPLAIN=considered 4914, overlap target 289, GC content failed 83, low tm 976, high tm 2660, ok 906 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=286.895207 PRIMER_LEFT_0_PENALTY=2.027407 PRIMER_RIGHT_0_PENALTY=284.867800 PRIMER_LEFT_0_SEQUENCE=CCCTTCTTCCTTCCCCAA PRIMER_RIGHT_0_SEQUENCE=CACTGTTCCCTCAGCCCG PRIMER_LEFT_0=280,18 PRIMER_RIGHT_0=515,18 PRIMER_LEFT_0_TM=59.973 PRIMER_RIGHT_0_TM=62.868 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_RIGHT_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=280.000000 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=2.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=236 = SEQUENCE_ID=F2_X13 SEQUENCE_TARGET=298,75 SEQUENCE_TEMPLATE=AACTTTTTAAAAGCATTATTCTGCCTGTTGGGTGGAGAATAGACTGTAGGTGGGCAAAGAATGAAGGAAACTAGTGGGTTCAGGAGCTCGAGCTAGAAGTGGTGAGAAGGGTTTGGATTTGGGGTCTATGCTGAAGGTAGAGCCGACAAGATTTGCTAGGATTGGATGTGTAGGGTGAGGAAGTGGGGACAGCAAGAATGACTGGAGGGGTAAGTGGACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTC PRIMER_LEFT_EXPLAIN=considered 3804, overlap target 289, GC content failed 12, low tm 652, high tm 1841, ok 1010 PRIMER_RIGHT_EXPLAIN=considered 3804, overlap target 289, low tm 873, high tm 1715, high end compl 5, ok 922 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=62.821994 PRIMER_LEFT_0_PENALTY=0.838952 PRIMER_RIGHT_0_PENALTY=61.983042 PRIMER_LEFT_0_SEQUENCE=CAGACGGCTGTTCTCTTTCA PRIMER_RIGHT_0_SEQUENCE=GCCCTGGGCTTTAGAGAAG PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=394,19 PRIMER_LEFT_0_TM=59.161 PRIMER_RIGHT_0_TM=59.017 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=60.000000 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=117 = SEQUENCE_ID=F2_X14 SEQUENCE_TARGET=298,148 SEQUENCE_TEMPLATE=ACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTCATATTCTGGGCTCCTGGAACCAATCCCGTGAAAGAATTATTTTTGTGTTTCTAAAACTATGGTTCCCAATAAAAGTGACTCTCAGCGAGCCTCAATGCTCCCAGTGCTATTCATGGGCAGCTCTCTGGGCTCAGGAAGAGCCAGTAATACTACTGGATAAAGAAGACTTAAGAATCCACCACCTGGTGCACGCTGGTAGTCCGAGCACTCGGGAGGCTGAGGTGGGAGGAT PRIMER_LEFT_EXPLAIN=considered 4475, overlap target 230, low tm 988, high tm 2162, ok 1095 PRIMER_RIGHT_EXPLAIN=considered 3944, overlap target 289, GC content failed 74, low tm 898, high tm 1848, high end compl 2, ok 833 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=24.449698 PRIMER_LEFT_0_PENALTY=4.108177 PRIMER_RIGHT_0_PENALTY=20.341522 PRIMER_LEFT_0_SEQUENCE=TTGGAAACCTCATCTTTCTTCTTC PRIMER_RIGHT_0_SEQUENCE=GAGCCCAGAATATGAGTGGC PRIMER_LEFT_0=274,24 PRIMER_RIGHT_0=466,20 PRIMER_LEFT_0_TM=60.108 PRIMER_RIGHT_0_TM=59.658 PRIMER_LEFT_0_GC_PERCENT=37.500 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=20.000000 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=193 = SEQUENCE_ID=F2R_X1 SEQUENCE_TARGET=235,90 SEQUENCE_TEMPLATE=CTTCCTCGCTCGCGCCCGCGCGACCGCGCGCCCCAGTCCCGCCCCGCCCCGCTAACCGCCCCAGACACAGCGCTCGCCGAGGGTCGCTTGGACCCTGATCTTACCCGTGGGCACCCTGCGCTCTGCCTGCCGCGAAGACCGGCTCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCGGAGCAGCCCGAGGCGGGGCAGCCTCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGGGCCGCGGCGGCTGCTGCTGGTGGCCGCCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGCCCGCACCCGGGCCCGCAGGCCAGGTGAGA PRIMER_LEFT_EXPLAIN=considered 2940, GC content failed 829, low tm 31, high tm 1984, ok 96 PRIMER_RIGHT_EXPLAIN=considered 951, overlap target 226, GC content failed 209, high tm 510, ok 6 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=57988.526397 PRIMER_LEFT_0_PENALTY=984.110493 PRIMER_RIGHT_0_PENALTY=57004.415904 PRIMER_LEFT_0_SEQUENCE=GAGGGTGAAGCGGAGCAG PRIMER_RIGHT_0_SEQUENCE=GCCGCACAGACTGAAGCA PRIMER_LEFT_0=168,18 PRIMER_RIGHT_0=285,18 PRIMER_LEFT_0_TM=62.110 PRIMER_RIGHT_0_TM=62.416 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=980.000000 PRIMER_RIGHT_0_POSITION_PENALTY=57000.000000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=118 = SEQUENCE_ID=F2R_X2-seg1 SEQUENCE_TARGET=4,702 SEQUENCE_TEMPLATE=TTTCAGAATCAAAAGCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACCCCAATGATAAATATGAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTGAATACAGATTAGTCTCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCATCTCAGAAGATGCCTCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTGTGTACACCGGAGTGTTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCATCCTGAAAATGAAGGTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGGCCACGGCAGATGTGCTGTTTGTGTCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATTGGCAGTTTGGGTCTGAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGTAACATGTACGCCTCTATCTTGCTCATGACAGTCATAAGCATTGACCGGTTTCTGGCTGTGGTGTATCCCATGCAGTCCCTCTCCTGGCGTACTCTGGGAAGGGCTTCCTTCACTTGTCTGGCCATCTGGGCTTTGGCCATCGCAGGGGTAGTGCCTCTCGTCCTCAAGGAGCAAACCATCCAGGTGCCCGGGCTCAACATCACTACCTGTCATGATGTGCTCAATGAAACCCTGCT PRIMER_LEFT_EXPLAIN=considered 6710, GC content failed 4, low tm 1539, high tm 3376, high repeat similarity 1, ok 1790 PRIMER_RIGHT_EXPLAIN=considered 6710, GC content failed 4, low tm 1540, high tm 3376, high repeat similarity 2, ok 1788 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=39005.267605 PRIMER_LEFT_0_PENALTY=19005.004751 PRIMER_RIGHT_0_PENALTY=20000.262854 PRIMER_LEFT_0_SEQUENCE=TTTCAGAATCAAAAGCAACAAAT PRIMER_RIGHT_0_SEQUENCE=AGCAGGGTTTCATTGAGCAC PRIMER_LEFT_0=0,23 PRIMER_RIGHT_0=705,20 PRIMER_LEFT_0_TM=57.995 PRIMER_RIGHT_0_TM=60.263 PRIMER_LEFT_0_GC_PERCENT=26.087 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=22.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=19000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=20000.000000 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=706 = SEQUENCE_ID=F2R_X2-seg2 SEQUENCE_TARGET=0,514 SEQUENCE_TEMPLATE=TGATGTGCTCAATGAAACCCTGCTCGAAGGCTACTATGCCTACTACTTCTCAGCCTTCTCTGCTGTCTTCTTTTTTGTGCCGCTGATCATTTCCACGGTCTGTTATGTGTCTATCATTCGATGTCTTAGCTCTTCCGCAGTTGCCAACCGCAGCAAGAAGTCCCGGGCTTTGTTCCTGTCAGCTGCTGTTTTCTGCATCTTCATCATTTGCTTCGGACCCACAAACGTCCTCCTGATTGCGCATTACTCATTCCTTTCTCACACTTCCACCACAGAGGCTGCCTACTTTGCCTACCTCCTCTGTGTCTGTGTCAGCAGCATAAGCTCGTGCATCGACCCCCTAATTTACTATTACGCTTCCTCTGAGTGCCAGAGGTACGTCTACAGTATCTTATGCTGCAAAGAAAGTTCCGATCCCAGCAGTTATAACAGCAGTGGGCAGTTGATGGCAAGTAAAATGGATACCTGCTCTAGTAACCTGAATAACAGCATATACAAAAAGCTGTTAACTTAGGAAAAGGGACTGCTGGGAGGTTAAAAAGAAAAGTTTATAAAAGTGAATAACCTGAGGATTCTATTAGTCCCCACCCAAACTTTATTGATTCACCTCCTAAAACAACAGATGTACGACTTGCATACCTGCTTTTTATGGGAGCTGTCAAGCATGTATTTTTGTCAATTACCAGAAAGATAACAGGACGAGATGACGGTGTTATTCCAAGGGAATATTGCCAATGCTACAGTAATAAA PRIMER_LEFT_EXPLAIN=considered 4999, overlap target 227, low tm 1458, high tm 1982, long poly-x seq 22, ok 1310 PRIMER_RIGHT_EXPLAIN=considered 6997, GC content failed 109, low tm 2483, high tm 2360, long poly-x seq 22, ok 2023 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=20001.710366 PRIMER_LEFT_0_PENALTY=20000.498267 PRIMER_RIGHT_0_PENALTY=1.212099 PRIMER_LEFT_0_SEQUENCE=TGATGTGCTCAATGAAACCC PRIMER_RIGHT_0_SEQUENCE=CTCCCAGCAGTCCCTTTTC PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=532,19 PRIMER_LEFT_0_TM=59.502 PRIMER_RIGHT_0_TM=59.788 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=20000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=533 = SEQUENCE_ID=F3_X1 SEQUENCE_TARGET=298,104 SEQUENCE_TEMPLATE=ACCGGGAGGAGGCGGGGCAGGGGTGTGGACTCGCCGGGGGCCGCCCACCGCGACGGCAAGTGACCCGGGCCGGGGGCGGGGAGTCGGGAGGAGCGGCGGGGGCGGGCGCCGGGGGCGGGCAGAGGCGCGGGAGAGCGCGCCGCCGGCCCTTTATAGCGCGCGGGGCACCGGCTCCCCAAGACTGCGAGCTCCCCGCACCCCCTCGCACTCCCTCTGGCCGGCCCAGGGCGCCTTCAGCCCAACCTCCCCAGCCCCACGGGCGCCACGGAACCCGCTCGATCTCGCCGCCAACTGGTAGACATGGAGACCCCTGCCTGGCCCCGGGTCCCGCGCCCCGAGACCGCCGTCGCTCGGACGCTCCTGCTCGGCTGGGTCTTCGCCCAGGTGGCCGGCGCTTCAGGTGAGTGGCACCAGCCCCTGGAAGCCCGGGGCGCGCCACACGCAGGAGGGAGGCGACAGTCCTGGCTGGCAGCGGGCTCGCCCTGGTTCCCCGGGGCGCCCATGTTGTCCCCCGCGCCTACGGGACTCGGCTGCGCTCACCCAGCCCGGCTTGAATGAACCGAGTCCGTCGGGCGCCGGCGGGAGTTGCAGGGAGGGAGTTGGCGCCCCAGACCCCGCTGCCCCTTCCGCTGGAGAGTTTTGCTCGGGGTGTCCGAGTAATTGGACTGTTGTTGCATAAGCGGACTTTTAGCTCCCGCTT PRIMER_LEFT_EXPLAIN=considered 4094, overlap target 289, GC content failed 1473, low tm 23, high tm 2263, ok 46 PRIMER_RIGHT_EXPLAIN=considered 4094, overlap target 289, GC content failed 716, low tm 101, high tm 2783, ok 205 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=808.329006 PRIMER_LEFT_0_PENALTY=4.915955 PRIMER_RIGHT_0_PENALTY=803.413051 PRIMER_LEFT_0_SEQUENCE=CTCGCCGCCAACTGGTAG PRIMER_RIGHT_0_SEQUENCE=ACTGTCGCCTCCCTCCTG PRIMER_LEFT_0=280,18 PRIMER_RIGHT_0=459,18 PRIMER_LEFT_0_TM=62.916 PRIMER_RIGHT_0_TM=61.413 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=800.000000 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=180 = SEQUENCE_ID=F3_X2 SEQUENCE_TARGET=298,116 SEQUENCE_TEMPLATE=CTCTGGGCTCTTCCCGGTCAAGTTATAACAAGGCTTTGCCCATGAATAATTTCAAACGAAAATGTCAAGATCCTTGCCGGTGTCCTGGGATTACAAGGTGAATCTTGTCATGAAGAAATTCTAGGTCTAGAAAAAATTTGAAGATTCTTTTTCTCTTGATAATTCACTAATGAAGCTTTTGTGGTTGAAAAATAAAAAGTGAGGTTTATGGTGATGTCAGGTGGGAAGGTGTTTTATACATCAATACATTCGAGTGCTCTGAAGTGCATGTAATAATAGCTGTTTCTCTGTTGTTTAAAGGCACTACAAATACTGTGGCAGCATATAATTTAACTTGGAAATCAACTAATTTCAAGACAATTTTGGAGTGGGAACCCAAACCCGTCAATCAAGTCTACACTGTTCAAATAAGGTAAGCTGGGTACAGAAAAAGAAAATTAAGGTCTTTGATGTTTCTACTGTCCTATGCTGAACAAGAATGTCTTTAAAGCTGATTACTGGATGAAATTATTTAACAGATGACGAAGAAGAAGGGATTCTTGGCAATTCGCTGGCCGGTGTCATACTCTATTAGGCCTGCAACATTTCCAGACCTTAAACTGATAGAACATTTTAATTGTTTTAATTGTTTTTGGAAATGATGGGAGAGTTCCTAAGTGGAGTATAAACTGTGGAGAGATGAACCATCTTGAGTAGGCACTGAAGTGTGCTT PRIMER_LEFT_EXPLAIN=considered 4082, overlap target 289, GC content failed 83, low tm 1878, high tm 779, high repeat similarity 1, long poly-x seq 20, ok 1032 PRIMER_RIGHT_EXPLAIN=considered 4214, overlap target 289, GC content failed 232, low tm 1968, high tm 682, ok 1043 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=113.663477 PRIMER_LEFT_0_PENALTY=109.223369 PRIMER_RIGHT_0_PENALTY=4.440107 PRIMER_LEFT_0_SEQUENCE=CATGTAATAATAGCTGTTTCTCTGTTG PRIMER_RIGHT_0_SEQUENCE=TTTTCTTTTTCTGTACCCAGCTT PRIMER_LEFT_0=266,27 PRIMER_RIGHT_0=436,23 PRIMER_LEFT_0_TM=57.777 PRIMER_RIGHT_0_TM=58.560 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=100.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=171 = SEQUENCE_ID=F3_X3 SEQUENCE_TARGET=298,204 SEQUENCE_TEMPLATE=TGAGGACTCAAACATGCATCCCAAGTGCTCGGTGTTGCTAGGTATGATGGCTCACACCTGTACATTCAGCACTTTGGGAGGCCGAAGCAGAAGGATCAGCCTGGGCAACATAGCAGGACCCCATCTCTACAAAACAATGTTTAAAAAAAAGCAAAGTGCTCAGCACAGTGACTGCATCATTAGGATTGATTGTAGGGCTCCTGATGTTAGCACAGAACACCACAGCCAGGAAGCAGTCTATCTTGTTGGGTGCAAATTGTAACATTCCATTTATGTTTCTTCCTTCTTTTCTTTCTTTAGCACTAAGTCAGGAGATTGGAAAAGCAAATGCTTTTACACAACAGACACAGAGTGTGACCTCACCGACGAGATTGTGAAGGATGTGAAGCAGACGTACTTGGCACGGGTCTTCTCCTACCCGGCAGGGAATGTGGAGAGCACCGGTTCTGCTGGGGAGCCTCTGTATGAGAACTCCCCAGAGTTCACACCTTACCTGGAGAGTAAGTGGCTTGGGCTGTAATACCGTTCATTCTTGTTAGAAACGTCTGAACATTCTCGTGATCTTGTGCCTTTAGGGGCTACAAAATTAAAAATATTTATTCTTTTTTTCTCAGAAACTGGTATGTATCACAGCCCTCTTCACACATTCCAGATGTGGTAGGAGGTTCACAGAATGTGAACTTTTGGAGCTGATGACAGTGTCATCAAGTAACTTTCTCCCCCAGTCTGTCCCCAGACCCTGTTACTGTCCTCAGTAAGCGGCTGAATGTGTGTTGGGAGAGGGCGGGCCAGGGAAGCGG PRIMER_LEFT_EXPLAIN=considered 4965, overlap target 289, GC content failed 54, low tm 1212, high tm 2106, high repeat similarity 28, long poly-x seq 22, ok 1254 PRIMER_RIGHT_EXPLAIN=considered 5005, overlap target 289, GC content failed 185, low tm 1231, high tm 1945, long poly-x seq 11, ok 1344 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=209.077263 PRIMER_LEFT_0_PENALTY=206.472471 PRIMER_RIGHT_0_PENALTY=2.604792 PRIMER_LEFT_0_SEQUENCE=TTCCATTTATGTTTCTTCCTTCTT PRIMER_RIGHT_0_SEQUENCE=TATTACAGCCCAAGCCACTT PRIMER_LEFT_0=264,24 PRIMER_RIGHT_0=521,20 PRIMER_LEFT_0_TM=57.528 PRIMER_RIGHT_0_TM=57.395 PRIMER_LEFT_0_GC_PERCENT=29.167 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=200.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=258 = SEQUENCE_ID=F3_X4 SEQUENCE_TARGET=298,183 SEQUENCE_TEMPLATE=TGGTTAAATAAAATATATTATTAAAATTAATTTCACCTGTTCCTTTTTACTTTTTCTAATGTGACTACTAGAAAACTTAAAATGACATCTGAGGCTCCATTGTCTTCCCCTTGGGCCAGCACTACCACAGAATGTCTTAGGATTCAGCTCCAGGCCGCCACGCCTGCTTCTTTCAGGGAGCTGGTTCTATGCACATGTTTTATATGAGAGATAATTAAGTTGTCAATTGTGATAACAAAACAGGATTTGACTTTGTACAGAATTCTTTGGTTCCAACCAAGCTCATTTCCTTTGTTTCAGCAAACCTCGGACAGCCAACAATTCAGAGTTTTGAACAGGTGGGAACAAAAGTGAATGTGACCGTAGAAGATGAACGGACTTTAGTCAGAAGGAACAACACTTTCCTAAGCCTCCGGGATGTTTTTGGCAAGGACTTAATTTATACACTTTATTATTGGAAATCTTCAAGTTCAGGAAAGGTGAGCATTTTTTAATTTGTTTTTATGACCTGTTTTAAATTGTGAATACTTGGTTTTACAACCCATTTCTTCCCCAATTCAAAAATAGCAGAACAGAGTTGTTGAGAAGGTGATGGAGTAGAAGGGGGAGCGCGCACTGTGGGGAGGGGTGGACAACAGGCCTGGTCCTACCTGTGACTCTGCACTACCCTGTGACTCTGGCAGGGCCCCCTCGGAGACCCAGGTTCCTCAGCCAACCGGCTGGATCAGGTCATCTCTAAAGGTCCCGCCACGCTCACATTTCTCCCTCTATTGAGGATC PRIMER_LEFT_EXPLAIN=considered 4884, overlap target 289, GC content failed 320, low tm 1911, high tm 1322, high end compl 6, ok 1036 PRIMER_RIGHT_EXPLAIN=considered 4770, overlap target 289, GC content failed 176, low tm 1330, high tm 1913, long poly-x seq 21, ok 1041 PRIMER_PAIR_EXPLAIN=considered 36, high end compl 33, ok 3 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=330.158482 PRIMER_LEFT_0_PENALTY=2.108929 PRIMER_RIGHT_0_PENALTY=328.049552 PRIMER_LEFT_0_SEQUENCE=CCAAGCTCATTTCCTTTGTTTC PRIMER_RIGHT_0_SEQUENCE=TCACAATTTAAAACAGGTCATAAAAAC PRIMER_LEFT_0=276,22 PRIMER_RIGHT_0=523,27 PRIMER_LEFT_0_TM=60.109 PRIMER_RIGHT_0_TM=58.950 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=25.926 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=320.000000 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=248 = SEQUENCE_ID=F3_X5 SEQUENCE_TARGET=298,164 SEQUENCE_TEMPLATE=CAAAATTTGTTTTTGGTTCAATGCATAATACTCCCTTCCTTTTTCTTTTACTGCAGATATCTTCTAAAGGGGCTCAATAGGGTTCAATATGCCTAAATTGGATCTTCTCAGTCTTGGAAAAGGCATTTTTAGCAGTGATCAAGGGAAACTGATTAGCGAAGTCACTTCTAATCCTTCACGTGTCAGCTGTGTTCTTGTAGGCTTTGCTTAGAACCTAGGTTTTTACTTCCACAGTGACTTAATAAAGGGGAAAGAATTGACTCAGAGCCCAGATGAATTAAGAACTCTATCTTTTTACAGAAAACAGCCAAAACAAACACTAATGAGTTTTTGATTGATGTGGATAAAGGAGAAAACTACTGTTTCAGTGTTCAAGCAGTGATTCCCTCCCGAACAGTTAACCGGAAGAGTACAGACAGCCCGGTAGAGTGTATGGGCCAGGAGAAAGGGGAATTCAGAGGTGAGTGGCTCTGCCAGCCATTTGCCTGGGGGTATGGGTGCTGTGGGTGACTTCTGGAGGAGTAGCTCCACCCTCAGGGCTGGGATATACTTCCTTGGTTAAATATTCAGGAAAACAAACTGCCTGGAGGTTTTTTGTTGTTATTTGTTTGTTTTGGTTTTGATTTTGCTTTGGTACAAAAAAGATTTTGGACATTTAGAAATGTTTCTGTGTTGATTGTGCCCTTGTATTAGCAGGTGTTTTCTTGAGCACCTGTCATGTGCTAAGCCCTCTGCTGAGCACTGGATACACAAACTGTGT PRIMER_LEFT_EXPLAIN=considered 4694, overlap target 289, GC content failed 4, low tm 2035, high tm 844, ok 1522 PRIMER_RIGHT_EXPLAIN=considered 4397, overlap target 289, GC content failed 28, low tm 1323, high tm 1565, high end compl 4, long poly-x seq 44, ok 1144 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=194.838849 PRIMER_LEFT_0_PENALTY=69.931898 PRIMER_RIGHT_0_PENALTY=124.906952 PRIMER_LEFT_0_SEQUENCE=CCAGATGAATTAAGAACTCTATCTTTT PRIMER_RIGHT_0_SEQUENCE=GGCAAATGGCTGGCAGAG PRIMER_LEFT_0=268,27 PRIMER_RIGHT_0=485,18 PRIMER_LEFT_0_TM=57.068 PRIMER_RIGHT_0_TM=62.907 PRIMER_LEFT_0_GC_PERCENT=29.630 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=60.000000 PRIMER_RIGHT_0_POSITION_PENALTY=120.000000 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=218 = SEQUENCE_ID=F3_X6 SEQUENCE_TARGET=298,141 SEQUENCE_TEMPLATE=CAGCATCGTTTTAGGCACTTCTTTCCAGCCATTGTTCTTACCTCCAACTACATATCTTTTCTGGACTGTGCATTATTCAGTTTATTAAATGCCCATTAAATGTGTTTAGCCATTGTCAATTACTCTGAAACGTTCAGGTTTTGACAAATTCTTTCCTAATGTAAGTGTGGTGGAAAGAGTGAAAGAAAGTCAAATTGCACAAAAATAGGATGGTGTAATTTGGGGTTATGCCGTCAATTTTGTCCACTGATAAATGGGATTTGAGCTCTCCAAGTTGACTAGATGCCCTTTATTTTTCAGAAATATTCTACATCATTGGAGCTGTGGTATTTGTGGTCATCATCCTTGTCATCATCCTGGCTATATCTCTACACAAGTGTAGAAAGGCAGGAGTGGGGCAGAGCTGGAAGGAGAACTCCCCACTGAATGTTTCATAAAGGAAGCACTGTTGGAGCTACTGCAAATGCTATATTGCACTGTGACCGAGAACTTTTAAGAGGATAGAATACATGGAAACGCAAATGAGTATTTCGGAGCATGAAGACCCTGGAGTTCAAAAAACTCTTGATATGACCTGTTATTACCATTAGCATTCTGGTTTTGACATCAGCATTAGTCACTTTGAAATGTAACGAATGGTACTACAACCAATTCCAAGTTTTAATTTTTAACACCATGGCACCTTTTGCACATAACATGCTTTAGATTATATATTCCGCACTCAAGGAGTAACCAGG PRIMER_LEFT_EXPLAIN=considered 4464, overlap target 289, GC content failed 57, low tm 1831, high tm 878, ok 1409 PRIMER_RIGHT_EXPLAIN=considered 4311, overlap target 289, GC content failed 51, low tm 1766, high tm 895, long poly-x seq 22, ok 1288 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=9.218677 PRIMER_LEFT_0_PENALTY=5.991812 PRIMER_RIGHT_0_PENALTY=3.226865 PRIMER_LEFT_0_SEQUENCE=TTGACTAGATGCCCTTTATTTTTC PRIMER_RIGHT_0_SEQUENCE=CAGTAGCTCCAACAGTGCTTC PRIMER_LEFT_0=274,24 PRIMER_RIGHT_0=459,21 PRIMER_LEFT_0_TM=58.008 PRIMER_RIGHT_0_TM=57.773 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=186 = SEQUENCE_ID=F5_X10 SEQUENCE_TARGET=59,219 SEQUENCE_TEMPLATE=TTATTTATTATCATGAAATAACTTTGCAAATGAAAACAATTTTGAATATATTTTCTTTCAGGCAGGAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTATAAGTGGAACATCTTAGAGTTTGATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATACTACAGTGACGTGGACATCATGAGAGACATCGCCTCTGGGCTAATAGGACTACTTCTAATCTGTAAGAGCAGATCCCTGGACAGGCGAGGAATACAGGTATTTTGTCCTTGAAGTAACCTTTCAGAAATTCTGAGAATTT PRIMER_LEFT_EXPLAIN=considered 2597, overlap target 32, GC content failed 195, low tm 1010, high tm 658, high end compl 23, high repeat similarity 8, ok 671 PRIMER_RIGHT_EXPLAIN=considered 2435, overlap target 50, low tm 1068, high tm 632, ok 685 PRIMER_PAIR_EXPLAIN=considered 18, high end compl 16, ok 2 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=288.237505 PRIMER_LEFT_0_PENALTY=263.479042 PRIMER_RIGHT_0_PENALTY=24.758462 PRIMER_LEFT_0_SEQUENCE=TTGCAAATGAAAACAATTTTGAA PRIMER_RIGHT_0_SEQUENCE=TGAAAGGTTACTTCAAGGACAAAA PRIMER_LEFT_0=23,23 PRIMER_RIGHT_0=302,24 PRIMER_LEFT_0_TM=59.521 PRIMER_RIGHT_0_TM=59.242 PRIMER_LEFT_0_GC_PERCENT=21.739 PRIMER_RIGHT_0_GC_PERCENT=33.333 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=260.000000 PRIMER_RIGHT_0_POSITION_PENALTY=20.000000 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=280 = SEQUENCE_ID=F7_X1 SEQUENCE_TARGET=298,68 SEQUENCE_TEMPLATE=ATTTGAAGTGTTGGTGCCACACACACCTTAACACCTGCACGCTGGCAACAAAACCGTCCGCTCTGCAGCACAGCTGGGGTCACCTGACCTTTCTCCTGTCCCCCCCACTTGAGCTCAGTGGCTGGGCAGCAGGGGATGCATGGCCACTGGCGGCCAGGTGCAGCTCTCAGCTGGGGTGTTCAGAGGACGCCTGTGTCCTCCCCTCCCCCATCCCTCTGTCACCCTTGGAGGCAGAGAACTTTGCCCGTCAGTCCCATGGGGAATGTCAACAGGCAGGGGCAGCACTGCAGAGATTTCATCATGGTCTCCCAGGCCCTCAGGCTCCTCTGCCTTCTGCTTGGGCTTCAGGGCTGCCTGGCTGCAGGTGCGTCCGGGGAGGTTTTCTCCATAAACTTGGTGGAAGGGCAGTGGGCAAATCCAGGAGCCAGCCCGGGCTTCCCAAACCCCGCCCTTGCTCCGGACACCCCCATCCACCAGGAGGGTTTTCTGGCGGCTCCTGTTCAATTTCTTTCCTTCTAGAAACCAGCATCCAGGCACAGGAGGGGAGGCCCTTCTTGGTAGCCCAGGCTTTGGCGGGATTATTTTTCAAAGAACTTTAGGAGTGGGTGGTGCTTTCCTGGCCCCCATGGCCCTGCCTGTGAGGTCGGACAAGCGCAGGGAGTCT PRIMER_LEFT_EXPLAIN=considered 3572, overlap target 289, low tm 118, high tm 2723, long poly-x seq 23, ok 419 PRIMER_RIGHT_EXPLAIN=considered 3734, overlap target 289, GC content failed 17, low tm 289, high tm 2735, high end compl 1, ok 403 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=42.860415 PRIMER_LEFT_0_PENALTY=0.295503 PRIMER_RIGHT_0_PENALTY=42.564912 PRIMER_LEFT_0_SEQUENCE=GCAGCACTGCAGAGATTTCA PRIMER_RIGHT_0_SEQUENCE=GAGAAAACCTCCCCGGAC PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=385,18 PRIMER_LEFT_0_TM=60.296 PRIMER_RIGHT_0_TM=59.435 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=15.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=40.000000 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=9.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=108 = SEQUENCE_ID=F7_X2 SEQUENCE_TARGET=298,70 SEQUENCE_TEMPLATE=TGTGTGGGGTGTGGGGATAGGGTCAGGGGATGGCGTGGGGGGTGTGGCATGGGGATGGCACGTGTGGCATGGGGATGGGGATGGGGGGTGGGGCATGGCCGAGTGGGGCTGGGGCTGGGAATGGTGAGTGGGGCATGGGGATGGCGAGTAGGGGGTGTGGCGTGAGGATGGCTAGTGGGGCGTGGGGATGGCGTGTGGGGATGGCGAGTGGGGGGTGGGCTGTGAGGGACAGTGCCTGGGATGTGGCTGCAGCCCTAGCTCACAGCATGGCCTTATGACCCCGGCCACCTTCCTGCCCAGGCGGGGTCGCTAAGGCCTCAGGAGGAGAAACACGGGACATGCCGTGGAAGCCGGGGCCTCACAGAGGTGAGCAGGGACTGCCACTGGTTTTGTCCTGGGGCCCAGTGGGGGCAACATCACCTCCTTCCCCTCCCATGGCAAAGAGCCAGCCCGCGGGGTGGCTACTGCAGTGCCCCCCAAGGAGGGTGTTCCCTGCTCGAGAGGAAGTGACCGCTCCAGCTTGGCCTTCCCTGGGACTGGGGTGCAGGCGATTTTATCTTCTTTGCTCCATTCTGTTCCTTCCAGATAATCGTGTGTTCTTCATCAGGTTTTCCTCAGTTCTTGAGAGCTTTTCTGATGCAAATCTGCTTTCACCCCAGGGCGGTC PRIMER_LEFT_EXPLAIN=considered 3301, overlap target 289, GC content failed 28, low tm 14, high tm 2710, high end compl 1, long poly-x seq 65, ok 194 PRIMER_RIGHT_EXPLAIN=considered 3601, overlap target 289, GC content failed 16, low tm 390, high tm 2278, long poly-x seq 22, ok 606 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=306.817739 PRIMER_LEFT_0_PENALTY=304.241567 PRIMER_RIGHT_0_PENALTY=2.576172 PRIMER_LEFT_0_SEQUENCE=CATGGCCTTATGACCCCG PRIMER_RIGHT_0_SEQUENCE=CAGTGGCAGTCCCTGCTC PRIMER_LEFT_0=265,18 PRIMER_RIGHT_0=385,18 PRIMER_LEFT_0_TM=62.242 PRIMER_RIGHT_0_TM=60.576 PRIMER_LEFT_0_GC_PERCENT=61.111 PRIMER_RIGHT_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_PAIR_0_LIBRARY_MISPRIMING=15.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=300.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=12.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=121 = SEQUENCE_ID=F7_X2' SEQUENCE_TARGET=298,165 SEQUENCE_TEMPLATE=AGCCTCACGTTTACCGCGGCGCCCGCAGCCCCCTTCGCCCGCTTCCGCGCGTGCCCCCGAGCGCGCCCTCGGGATCAGCCCCCGGAAGCAGAGAGGCCAGGCCGGGAAGGATGGGCGAACGGGGTGGCTGACCCGGGAGCACGGCAGGGAGGACACCCAGCCAGGCCCGCGAGCAGCGCCGCTCCCCTCCTCCAGGACGGGCGGGAACCTGCGATGCCCCCGCCGCGTGGGCCGTGGGGCGGTCTCCGAGGCACTGGGCGGGGCACGCGGTGGGCGCTTCACGGAACTCGCATTTCCCAGTCTTCGTAACCCAGGAGGAAGCCCACGGCGTCCTGCACCGGCGCCGGCGCGCCAACGCGTTCCTGGAGGAGCTGCGGCCGGGCTCCCTGGAGAGGGAGTGCAAGGAGGAGCAGTGCTCCTTCGAGGAGGCCCGGGAGATCTTCAAGGACGCGGAGAGGACGGTGAGCCCAGCCTCGGGGCGCCCCGCGCGGACACTGCACGGCGGCGGTGAACCAGGCCGCGTGGGGCCGCCTGCGTCTCTTTGGCTGCGGCCTGTGGGCGGCGAACACGCAGCGGCGCCCGCGCGCGCGCTCTCTCTGCGGGGGTCGCTTTCCGCCCGGGGTGACTCCGCTTTCCTGGGCGATGCCCCCACCCCCAGGCACGCGCTCTCCCCGTGCGGCCGCACCGCGCATGCCGGTTTTCACATCAGAAAATACGATTTGCACAAGCACACTTAGGGTGTCCCCCTTAACTTCCCAAGG PRIMER_LEFT_EXPLAIN=considered 4704, overlap target 289, GC content failed 1097, low tm 35, high tm 3097, high end compl 6, ok 180 PRIMER_RIGHT_EXPLAIN=considered 4704, overlap target 289, GC content failed 1078, low tm 138, high tm 2874, ok 325 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=1385.802240 PRIMER_LEFT_0_PENALTY=62.138031 PRIMER_RIGHT_0_PENALTY=1323.664208 PRIMER_LEFT_0_SEQUENCE=CTTCACGGAACTCGCATTT PRIMER_RIGHT_0_SEQUENCE=AGCCAAAGAGACGCAGGC PRIMER_LEFT_0=276,19 PRIMER_RIGHT_0=546,18 PRIMER_LEFT_0_TM=58.862 PRIMER_RIGHT_0_TM=61.664 PRIMER_LEFT_0_GC_PERCENT=47.368 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=60.000000 PRIMER_RIGHT_0_POSITION_PENALTY=1320.000000 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=271 = SEQUENCE_ID=F7_X3 SEQUENCE_TARGET=298,29 SEQUENCE_TEMPLATE=CAGAGCCTGTGTACCTCGTCCCAGGTCCACAGCTCAGCGACAGAAGAGTCAGGGTTGAACCTCGGGTGTTCTGACTTGGGAGCAGGAAATGTGTGGTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATT PRIMER_LEFT_EXPLAIN=considered 3344, overlap target 289, GC content failed 2, low tm 424, high tm 1910, ok 719 PRIMER_RIGHT_EXPLAIN=considered 3344, overlap target 289, low tm 284, high tm 2312, high any compl 8, high end compl 13, high repeat similarity 7, ok 431 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=303.683583 PRIMER_LEFT_0_PENALTY=1.510814 PRIMER_RIGHT_0_PENALTY=302.172769 PRIMER_LEFT_0_SEQUENCE=GGCTTGCTCTCTTGTTCCTTC PRIMER_RIGHT_0_SEQUENCE=GTTGCAGGCAGGACTGGT PRIMER_LEFT_0=277,21 PRIMER_RIGHT_0=359,18 PRIMER_LEFT_0_TM=60.511 PRIMER_RIGHT_0_TM=59.827 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MIR2 Repetitive element - a consensus PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=300.000000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=83 = SEQUENCE_ID=F7_X4 SEQUENCE_TARGET=298,118 SEQUENCE_TEMPLATE=GTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATTCAGGGCTCAGCCCAGTTGGAAATTGGTCTAGGTGACCTTTAAATCCCTTCCAGTCTGAGGTCTTTGACAGGGACCCAAGGTTCTGATTATCAGACTCAGTGGCCCCCTTCGCGGTCCCGGCCCTGGGCAACTTCTCAGCCCTGGAGACTGGCCCAGTTGAGAGTCCCTGTGTCCCGTGTGCCCA PRIMER_LEFT_EXPLAIN=considered 4234, overlap target 289, GC content failed 2, low tm 537, high tm 2588, ok 818 PRIMER_RIGHT_EXPLAIN=considered 4234, overlap target 289, GC content failed 81, low tm 378, high tm 2891, ok 595 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=6.323804 PRIMER_LEFT_0_PENALTY=2.096539 PRIMER_RIGHT_0_PENALTY=4.227266 PRIMER_LEFT_0_SEQUENCE=GACACCAGCCCACTCCAC PRIMER_RIGHT_0_SEQUENCE=GACCCAAAGTGGGGCCTT PRIMER_LEFT_0=280,18 PRIMER_RIGHT_0=433,18 PRIMER_LEFT_0_TM=60.097 PRIMER_RIGHT_0_TM=62.227 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=154 = SEQUENCE_ID=F7_X5 SEQUENCE_TARGET=298,145 SEQUENCE_TEMPLATE=GCTCGGAAGAGTGGTCACCCTGGAGGCCACTGCCCACCAGCCAGGCACCCCCCAAATGCAACCGCAGCCAGCACTGCCAGCCACTGGCAAGGCTGTTCAGACATGTGGCTCCTCTGATCCACGCCTTGTCCTTTGGATCAGTCCACGGAGCAGTGTGCCAAGCTCAGGCTCTGTCACCCACAGCTCATGCCACCTTCCAGGCAGAACACCACTGCTGACCCAGGGGCATGGCCACCCCGGGGGCTGGCGTCTCGCTGACCCCCAGAAGCCCCTCTCAGGGTGTCCCCTTCCTGTCCCCAGACAAGGATGACCAGCTGATCTGTGTGAACGAGAACGGCGGCTGTGAGCAGTACTGCAGTGACCACACGGGCACCAAGCGCTCCTGTCGGTGCCACGAGGGGTACTCTCTGCTGGCAGACGGGGTGTCCTGCACACCCACAGGTGACCAGGCTTCATGTCCCAGTCCCAGATGACACCAGTCCCTGTCCCACTAGGATTATCTTACTGGACAAAAGACGGGTGGGACTGGCCTTCACATCTACTGAGCACTAACTATGCACTGACCAATTGTGAGGTGGGATCTGGGCACCAAGGGTGGCACAGGCCAGCAGCGACCAGTGACTAGGATGGGCACCCTGGGGGCAATCCCTGAATGGCCTCAGGCCCCCTGCCAACTTCTAGGCAGACCAGGGGAGCCAAGCAAGGCACTATCTCACGTCCAACTGCCCACTCGCAGGAATC PRIMER_LEFT_EXPLAIN=considered 4351, overlap target 289, GC content failed 139, low tm 152, high tm 3259, long poly-x seq 22, ok 490 PRIMER_RIGHT_EXPLAIN=considered 4504, overlap target 289, GC content failed 1, low tm 499, high tm 3030, high repeat similarity 2, ok 683 PRIMER_PAIR_EXPLAIN=considered 20, high any compl 8, high end compl 10, ok 2 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=84.356932 PRIMER_LEFT_0_PENALTY=62.311131 PRIMER_RIGHT_0_PENALTY=22.045801 PRIMER_LEFT_0_SEQUENCE=GGGTGTCCCCTTCCTGTC PRIMER_RIGHT_0_SEQUENCE=TGGGACATGAAGCCTGGT PRIMER_LEFT_0=277,18 PRIMER_RIGHT_0=461,18 PRIMER_LEFT_0_TM=60.311 PRIMER_RIGHT_0_TM=60.046 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=55.556 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=60.000000 PRIMER_RIGHT_0_POSITION_PENALTY=20.000000 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=185 = SEQUENCE_ID=F7_X6 SEQUENCE_TARGET=298,114 SEQUENCE_TEMPLATE=GAAAAGTGAGCAGAGGGCGGTTTTAAGACGTAAGCCCTCTGTTTCCTCCAAAACCAGCCCTGACCATTGTCTCCTCAGCCAGCCACTTCTTCAAGGGCCTCTCATGGCCGGGCCCCACCAGTCAGGCCCAGCCGAGGCCCTGCCTTCCACCACCCCTGGGCCCTGGGAGCTCCTGCTCCTGGGGGCCTCCCATAGCCTCGGCCTCAAGGCCTCTCAGAGGATGGGTGTTTCTGAATCTTTCCTAGTGGCACGTTCATCCCTCACAAATCTCTGCATCTTTCTGACTTTTGTTTTACACAGTTGAATATCCATGTGGAAAAATACCTATTCTAGAAAAAAGAAATGCCAGCAAACCCCAAGGCCGAATTGTGGGGGGCAAGGTGTGCCCCAAAGGGGAGTGTCCATGGCAGGTAAGGCTTCCCCTGGCTTCAGGATTCCAAGCCCTGAGGGTCTTGAAGCCTTTTGAATGTGAACAACAGCTCTGGAAGGGAAAATGGGCAGGTCAGCCCAAGCCCACAGGCTCCAAGTCAGCACACCTAGCACCTCCAGCTCGCGGCACCCCCATGCTTTTAGTGGGGCAAGGAAGGAGAAAAGAAAACGACACTCACTGAGGGTCTACCCTGTGCAGAGAACCCTGCGAGATGCCCCATCCGAGTTGTCACGTCGTCCTCACGGTTACTCTTTGAGGTGGGATCTTTGCCTGATCTTTG PRIMER_LEFT_EXPLAIN=considered 3891, overlap target 289, GC content failed 43, low tm 599, high tm 2265, long poly-x seq 44, ok 651 PRIMER_RIGHT_EXPLAIN=considered 3894, overlap target 289, GC content failed 11, low tm 463, high tm 2371, high end compl 1, long poly-x seq 44, ok 715 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=88.837406 PRIMER_LEFT_0_PENALTY=6.324793 PRIMER_RIGHT_0_PENALTY=82.512614 PRIMER_LEFT_0_SEQUENCE=GCATCTTTCTGACTTTTGTTTTACAC PRIMER_RIGHT_0_SEQUENCE=ATCCTGAAGCCAGGGGAAG PRIMER_LEFT_0=272,26 PRIMER_RIGHT_0=434,19 PRIMER_LEFT_0_TM=59.675 PRIMER_RIGHT_0_TM=61.513 PRIMER_LEFT_0_GC_PERCENT=34.615 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=80.000000 PRIMER_LEFT_0_END_STABILITY=5.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=163 = SEQUENCE_ID=F7_X7 SEQUENCE_TARGET=298,128 SEQUENCE_TEMPLATE=CTTTGCAAAATCAGGAGCATTGGATCAAAGCTATGTGAAGATCCTGTGAGGTGAACAGTGAAATCTCACAGCGACATTTGTATTCTTGGGCCGTGCCCAAGAGCACGTCTCGGCTAGAGAGGGGCACAGCCTCCCAGAGCCAGGTCTGAGCAGCTTTGCCTGGGAGGGATCTGCAAAGACCCCAGGATTTCAGAAAGAAATTGTGCAATGCCAGAGGTTCCTTGGCATGCCCGGGAGGGCGAGTCATCAGAGAAACAATGACAGCAATGTGACTTCCACACCTCCTGTCCCCCCGCCCAGGTCCTGTTGTTGGTGAATGGAGCTCAGTTGTGTGGGGGGACCCTGATCAACACCATCTGGGTGGTCTCCGCGGCCCACTGTTTCGACAAAATCAAGAACTGGAGGAACCTGATCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTTCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCGCTGTCCGACCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCTGACCGCAGCTCTCAAGTGTCTCAGGGGCTGTGGCTCTGGGCTTCGTGCTGTCACTTCCACAGACAGACAGACATCCCCAAAAGGGGAGCAAC PRIMER_LEFT_EXPLAIN=considered 4032, overlap target 289, GC content failed 6, low tm 577, high tm 2278, long poly-x seq 44, ok 838 PRIMER_RIGHT_EXPLAIN=considered 4181, overlap target 289, GC content failed 10, low tm 236, high tm 3155, high end compl 5, long poly-x seq 22, ok 464 PRIMER_PAIR_EXPLAIN=considered 126, high any compl 28, high end compl 97, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=363.438040 PRIMER_LEFT_0_PENALTY=301.362873 PRIMER_RIGHT_0_PENALTY=62.075168 PRIMER_LEFT_0_SEQUENCE=AGCAATGTGACTTCCACACCT PRIMER_RIGHT_0_SEQUENCE=CGGACAGGGGAGAGTGGTA PRIMER_LEFT_0=262,21 PRIMER_RIGHT_0=447,19 PRIMER_LEFT_0_TM=59.637 PRIMER_RIGHT_0_TM=61.075 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=300.000000 PRIMER_RIGHT_0_POSITION_PENALTY=60.000000 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=186 = SEQUENCE_ID=F7_X8 SEQUENCE_TARGET=298,600 SEQUENCE_TEMPLATE=GCCGGGAAGAACTGTGGAGAATCGGGGCACGCCCTGTCCTCCCAGCTGCCAGGGCACAGCATCCCTTCCCCACCTGCAACACCCAGACCCCAGATTCACCCCAGTTCACTTGTCCCCACACGAGCCACAGGCTGCCACCTGGGGCAGGCTGGCCCACCTTGGGGTTAGATGCAGGTCCCCTTGCCCCAGAAGGAGACTGCAGCCCCTGCAGACCTAGAAATGGCCACAGCCCATCCCCATGCACCAGGGGGTGAGGTGGCAGGTGGTGGAAAGGGCCTGAGGGGGGCTTCTTCCTTCCAGGCGAGCACGACCTCAGCGAGCACGACGGGGATGAGCAGAGCCGGCGGGTGGCGCAGGTCATCATCCCCAGCACGTACGTCCCGGGCACCACCAACCACGACATCGCGCTGCTCCGCCTGCACCAGCCCGTGGTCCTCACTGACCATGTGGTGCCCCTCTGCCTGCCCGAACGGACGTTCTCTGAGAGGACGCTGGCCTTCGTGCGCTTCTCATTGGTCAGCGGCTGGGGCCAGCTGCTGGACCGTGGCGCCACGGCCCTGGAGCTCATGGTCCTCAACGTGCCCCGGCTGATGACCCAGGACTGCCTGCAGCAGTCACGGAAGGTGGGAGACTCCCCAAATATCACGGAGTACATGTTCTGTGCCGGCTACTCGGATGGCAGCAAGGACTCCTGCAAGGGGGACAGTGGAGGCCCACATGCCACCCACTACCGGGGCACGTGGTACCTGACGGGCATCGTCAGCTGGGGCCAGGGCTGCGCAACCGTGGGCCACTTTGGGGTGTACACCAGGGTCTCCCAGTACATCGAGTGGCTGCAAAAGCTCATGCGCTCAGAGCCACGCCCAGGAGTCCTCCTGCGAGCCCCATTTCCCTAGCCCAGCAGCCCTGGCCTGTGGAGAGAAAGCCAAGGCTGCGTCGAACTGTCCTGGCACCAAATCCCATATATTCTTCTGCAGTTAATGGGGTAGAGGAGGGCATGGGAGGGAGGGAGAGGTGGGGAGGGAGACAGAGACAGAAACAGAGAGAGACAGAGACAGAGAGAGACTGAGGGAGAGACTCTGAGGACATGGAGAGAGACTCAAAGAGACTCCAAGATTCAAAGAGACTAATAGAGACACAGAGATGGAATAGAAAAGATGAGAGGCAGAGGCAGACAGGCGCTGGACAGAGGGGCA PRIMER_LEFT_EXPLAIN=considered 8901, overlap target 289, GC content failed 87, low tm 201, high tm 7523, long poly-x seq 22, ok 779 PRIMER_RIGHT_EXPLAIN=considered 9054, overlap target 289, GC content failed 79, low tm 1105, high tm 6374, high end compl 1, ok 1206 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=447.046454 PRIMER_LEFT_0_PENALTY=304.949476 PRIMER_RIGHT_0_PENALTY=142.096979 PRIMER_LEFT_0_SEQUENCE=GTGGAAAGGGCCTGAGGG PRIMER_RIGHT_0_SEQUENCE=TTCTCTCCACAGGCCAGG PRIMER_LEFT_0=265,18 PRIMER_RIGHT_0=922,18 PRIMER_LEFT_0_TM=62.949 PRIMER_RIGHT_0_TM=59.903 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse MIR2 Repetitive element - a consensus PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=300.000000 PRIMER_RIGHT_0_POSITION_PENALTY=140.000000 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=658 = SEQUENCE_ID=HCF2_X2 SEQUENCE_TARGET=298,909 SEQUENCE_TEMPLATE=CTCCTCCCTGGGATGCCTGAGCTCCCAGGATTCTAGAAGGTTAGTTTTGCAAACCTTTAAAGAAGGGATTTTCATCAAGGGGCCCACAGATCCTTCATTGAGGTTTATGAGTCCCACATCAAAGGTTGGGTGTCTATCTACATCAGATTCTCTTAAAGTCCATGATCCTAAAACAGTTAAGAACTAATGCTGTGAGGGCCTCTTCCTGGGTCAAAGCCACAGGGAACCTGCCATGTGGATGCTGCAGCGGGGTGTGGATCAGCCAGGCCGCCTTTCACTGTGTTCTGTTTTCCCTCCCAGCTTTAGCTCCGCCAAAATGAAACACTCATTAAACGCACTTCTCATTTTCCTCATCATAACATCTGCGTGGGGTGGGAGCAAAGGCCCGCTGGATCAGCTAGAGAAAGGAGGGGAAACTGCTCAGTCTGCAGATCCCCAGTGGGAGCAGTTAAATAACAAAAACCTGAGCATGCCTCTTCTCCCTGCCGACTTCCACAAGGAAAACACCGTCACCAACGACTGGATTCCAGAGGGGGAGGAGGACGACGACTATCTGGACCTGGAGAAGATATTCAGTGAAGACGACGACTACATCGACATCGTCGACAGTCTGTCAGTTTCCCCGACAGACTCTGATGTGAGTGCTGGGAACATCCTCCAGCTTTTTCATGGCAAGAGCCGGATCCAGCGTCTTAACATCCTCAACGCCAAGTTCGCTTTCAACCTCTACCGAGTGCTGAAAGACCAGGTCAACACTTTCGATAACATCTTCATAGCACCCGTTGGCATTTCTACTGCGATGGGTATGATTTCCTTAGGTCTGAAGGGAGAGACCCATGAACAAGTGCACTCGATTTTGCATTTTAAAGACTTTGTTAATGCCAGCAGCAAGTATGAAATCACGACCATTCATAATCTCTTCCGTAAGCTGACTCATCGCCTCTTCAGGAGGAATTTTGGGTACACACTGCGGTCAGTCAATGACCTTTATATCCAGAAGCAGTTTCCAATCCTGCTTGACTTCAAAACTAAAGTAAGAGAGTATTACTTTGCTGAGGCCCAGATAGCTGACTTCTCAGACCCTGCCTTCATATCAAAAACCAACAACCACATCATGAAGCTCACCAAGGGCCTCATAAAAGATGCTCTGGAGAATATAGACCCTGCTACCCAGATGATGATTCTCAACTGCATCTACTTCAAAGGTAAGAGGCACCTTTACAGTTCTCACAGCAAACCCACAACATACTATTTTTGTATGTGGGTAGATTGAATGCCAAGAACTGTACTGTAGCTATAATTTATCCAGGAAAACTAGACACAAGATTGACTCTGGAACGGGGACAGGGAAGGCCAAGCTGAAGTGACAGTAGCATCTGACACTTACTGAGCCCTAACTCTGTGCTTTAACACAGCCTTGTGAGGTCATCACTGTTATTAGCATCCCCATTTTACAGAGGAAGCCACCAACACATGAAGTAAAAGGATGGGCTGGGCGCGGTGGC PRIMER_LEFT_EXPLAIN=considered 12144, overlap target 289, GC content failed 12, low tm 3242, high tm 5342, ok 3259 PRIMER_RIGHT_EXPLAIN=considered 12144, overlap target 289, GC content failed 15, low tm 3651, high tm 4687, high end compl 7, ok 3495 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=43.762258 PRIMER_LEFT_0_PENALTY=0.056936 PRIMER_RIGHT_0_PENALTY=43.705322 PRIMER_LEFT_0_SEQUENCE=TGTGTTCTGTTTTCCCTCCC PRIMER_RIGHT_0_SEQUENCE=TGTGAGAACTGTAAAGGTGCCTC PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=1231,23 PRIMER_LEFT_0_TM=59.943 PRIMER_RIGHT_0_TM=60.705 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=47.826 PRIMER_LEFT_0_SELF_ANY=1.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=40.000000 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=954 = SEQUENCE_ID=HCF2_X3 SEQUENCE_TARGET=298,278 SEQUENCE_TEMPLATE=TTTGACAGATGTGACAGATGAAGAAATAGAAGCGAGTTAGGTGCCTTACCATGGTCAAACAACTAGTTCGTATCAGACCCTACTCCAGAAACTATTCCAGTCCGGGTAACCTCTCGTTAACCTCTCTTGTTAGAAATGCAAATTTCTGCCCAAATCAGGCCTCAGGAATCAAGAGACTGTGGGGTCGGCTCTGCAGGCTATCTGAATGAGGCCTCCAGGGAAATCAGATTCACTCTCAAGGGTGAGACGATTTCCCTAAAGGAACCTTCTCATAACAGCCTCTTCCTGTGGCCTTTACAGGATCCTGGGTGAATAAATTCCCAGTGGAAATGACACACAACCACAACTTCCGGCTGAATGAGAGAGAGGTAGTTAAGGTTTCCATGATGCAGACCAAGGGGAACTTCCTCGCAGCAAATGACCAGGAGCTGGACTGCGACATCCTCCAGCTGGAATACGTGGGGGGCATCAGCATGCTAATTGTGGTCCCACACAAGATGTCTGGGATGAAGACCCTCGAAGCGCAACTGACACCCCGGGTGGTGGAGAGATGGCAAAAAAGCATGACAAACAGGTATTTCACACTGTGTGTTTGTTCTTTTGAGCTCCCAGATGCTGGGGGTGTCTGGGAATACTGGAAAATGGATCATTTTTTTAAAAAGGGAGAATTATGTACAAGTACCCAAGAACTTCCATACAGGGCCACTCTGTTAATTCAGCCCCAATTTGTTGCTTGAGATAAGAGATGATTAGAGAGCATTCATAAGGGACACATCTGCCCTCTAGGGGCCAGTTTCAGAAGTTAGAGGCAGATGACTTAGAGACAGCTTGGTGCTTGCTTTGTGGCTTCGAGTCCCAGCTTCATCATCCCTAA PRIMER_LEFT_EXPLAIN=considered 5543, overlap target 289, low tm 1266, high tm 2461, long poly-x seq 38, ok 1489 PRIMER_RIGHT_EXPLAIN=considered 5393, overlap target 289, GC content failed 28, low tm 1331, high tm 2335, high any compl 19, high end compl 7, long poly-x seq 67, ok 1317 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=145.727655 PRIMER_LEFT_0_PENALTY=1.210543 PRIMER_RIGHT_0_PENALTY=144.517111 PRIMER_LEFT_0_SEQUENCE=CCTCTTCCTGTGGCCTTTAC PRIMER_RIGHT_0_SEQUENCE=GCTCAAAAGAACAAACACACAGT PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=605,23 PRIMER_LEFT_0_TM=58.789 PRIMER_RIGHT_0_TM=58.483 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=39.130 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=140.000000 PRIMER_LEFT_0_END_STABILITY=6.0000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=328 = SEQUENCE_ID=HCF2_X4 SEQUENCE_TARGET=298,149 SEQUENCE_TEMPLATE=ATGCCTTTTTAAACCATAAACCAGGAAAAGTTCTGCTGCTAACCTTGAAGATAGGAAACGAACCATACAGTCTCAAGGAAATAATCATGCAACAGAAAACACACCTCAGTTTTCAGTAGCGGAATTACAAAGGAGTGTGCTTCCTAAAATCCTCAACTGACAGTCCCGGAATATAAATTTTAATAAGTGCTATATCAATTCTGTGATAAATATAACCCGTGGCCCTTTAAAGGGAAAATCATGATTCTTTTGTAACTTGTGGTTCAATAAAACTGGGCCCCCCTTTCCTTTTCTGTCTAGAACTCGAGAAGTGCTTCTGCCGAAATTCAAGCTGGAGAAGAACTACAATCTAGTGGAGTCCCTGAAGTTGATGGGGATCAGGATGCTGTTTGACAAAAATGGCAACATGGCAGGCATCTCAGACCAAAGGATCGCCATCGACCTGGTAACCACTCCCTTGTCCACCCCCGACCCGTCCCCAGGGTCTGCCTCAGCACAGCCCCACCTCCACTTGCCCTTCCTACCCACCCCCCAATCTCATGTCCCAGCTTGGGGTGCTGAGTCTGCTCTTCGGCCTGGGTGGGATACACAGAATGCCTAGTTTCATGGATGCCAGCTGGAGAGCACGGCACCTGGCAGACACTTACTGGGCAGGGGGGATCCCAAGAGCAGCCATGGGGTGAGCCCCACTCCCGCTGACACCAGAGACAGGGGAGACATGTGCTGCGGTCTGGGAAATAGCTAC PRIMER_LEFT_EXPLAIN=considered 4391, overlap target 289, GC content failed 128, low tm 1570, high tm 1096, long poly-x seq 22, ok 1286 PRIMER_RIGHT_EXPLAIN=considered 4238, overlap target 289, GC content failed 20, low tm 420, high tm 2770, long poly-x seq 44, ok 695 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=1.650642 PRIMER_LEFT_0_PENALTY=0.451656 PRIMER_RIGHT_0_PENALTY=1.198986 PRIMER_LEFT_0_SEQUENCE=CCCCCTTTCCTTTTCTGTCT PRIMER_RIGHT_0_SEQUENCE=GGTGGACAAGGGAGTGGTT PRIMER_LEFT_0=278,20 PRIMER_RIGHT_0=465,19 PRIMER_LEFT_0_TM=59.548 PRIMER_RIGHT_0_TM=59.801 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=1.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, MIR2 Repetitive element - a consensus PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=188 = SEQUENCE_ID=HCF2_X5 SEQUENCE_TARGET=298,850 SEQUENCE_TEMPLATE=CCCGGAGAAGTGCGCAGCAGTGTGGGGAGCTGGAGCTGGGGTGGCTGTCCTGCACCAGCCCCCACGACCCTCAGACCACAGGCACTGCCAAGAGGGAACATGAACCTAGCCGGCCTCTAAGTGCAACGGCTGCCCCTGACAGGTGGTGACAGATATTTTCAAGAGTGACTCTGACCAGCTGTGATTTCCACCTTACATGTTGTCTTTGGATCCTTTCCCTGAATGATATGAGATTGTGCTGGGAACTCTAGCCCTCTGTGTGCTGACCTCCAGAATCTGACAACTTTCCTTTCCAAACAGTTCAAGCACCAAGGCACGATCACAGTGAACGAGGAAGGCACCCAAGCCACCACTGTGACCACGGTGGGGTTCATGCCGCTGTCCACCCAAGTCCGCTTCACTGTCGACCGCCCCTTTCTTTTCCTCATCTACGAGCATCGCACCAGCTGCCTGCTCTTCATGGGAAGAGTGGCCAACCCCAGCAGGTCCTAGAGGTGGAGGTCTAGGTGTCTGAAGTGCCTTGGGGGCACCCTCATTTTGTTTCCATTCCAACAACGAGAACAGAGATGTTCTGGCATCATTTACGTAGTTTACGCTACCAATCTGAATTCGAGGCCCATATGAGAGGAGCTTAGAAACGACCAAGAAGAGAGGCTTGTTGGAATCAATTCTGCACAATAGCCCATGCTGTAAGCTCATAGAAGTCACTGTAACTGTAGTGTGTCTGCTGTTACCTAGAGGGTCTCACCTCCCCACTCTTCACAGCAAACCTGAGCAGCGCGTCCTAAGCACCTCCCGCTCCGGTGACCCCATCCTTGCACACCTGACTCTGTCACTCAAGCCTTTCTCCACCAGGCCCCTCATCTGAATACCAAGCACAGAAATGAGTGGTGTGACTAATTCCTTACCTCTCCCAAGGAGGGTACACAACTAGCACCATTCTTGATGTCCAGGGAAGAAGCCACCTCAAGACATATGAGGGGTGCCCTGGGCTAATGTTAGGGCTTAATTTTCTCAAAGCCTGACCTTTCAAATCCATGATGAATGCCATCAGTCCCTCCTGCTGTTGCCTCCCTGTGACCTGGAGGACAGTGTGTGCCATGTCTCCCATACTAGAGATAAATAAATGTAGCCACATTTACTGTGTATCTGTTATAATTCTCTATTTTTTGAAGCTCAAATATCAAAAGCCAAATCCAAATTCCTGGATAACTCCAGGTATGATAAAGGCTGAGAGGAAGTCACTTGAGCACCACAATGTGCCACAGCAGGGCATGTTCTCAGGACAGGACAGGTGTGTGCTGAATCCTGGGGAGGGTCTGTGCAGTACCCCAGAACTGTGGGGTGCTAAGTGGCACACAAGCCCCAGGGCTCCCACAGTCTATGCCAGGCTGCTGCAGCTTTCATCCCTCATACCTGGTCCTGCAGTGGGTCTGGTTTGACAGA PRIMER_LEFT_EXPLAIN=considered 11554, overlap target 289, GC content failed 7, low tm 2490, high tm 6121, ok 2647 PRIMER_RIGHT_EXPLAIN=considered 11432, overlap target 289, GC content failed 57, low tm 2518, high tm 5947, high end compl 12, long poly-x seq 20, ok 2589 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=466.578446 PRIMER_LEFT_0_PENALTY=3.339047 PRIMER_RIGHT_0_PENALTY=463.239398 PRIMER_LEFT_0_SEQUENCE=TCTGACAACTTTCCTTTCCAAAC PRIMER_RIGHT_0_SEQUENCE=TTGGCTTTTGATATTTGAGCTTC PRIMER_LEFT_0=275,23 PRIMER_RIGHT_0=1193,23 PRIMER_LEFT_0_TM=59.661 PRIMER_RIGHT_0_TM=59.761 PRIMER_LEFT_0_GC_PERCENT=39.130 PRIMER_RIGHT_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=460.000000 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=919 = SEQUENCE_ID=HMGCR_X1 SEQUENCE_TARGET=74,164 SEQUENCE_TEMPLATE=CCAGGCTGAGCGTCGGCGCCGGGGTTCGGTGGCCTCTAGTGAGATCTGGAGGATCCAAGGATTCTGTAGCTACAATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCCATCCCTGGGAAGTCATAGTGGGGACAGTGACACTGACCATCTGCATGATGTCCATGAACATGTTTACTGGTAACAATAAGATCTGTGGTTGGAATTATGAATGTCCAAAGTTTGAAGA PRIMER_LEFT_EXPLAIN=considered 2030, GC content failed 67, low tm 611, high tm 842, high any compl 3, high end compl 38, ok 469 PRIMER_RIGHT_EXPLAIN=considered 1490, overlap target 65, low tm 478, high tm 584, ok 363 PRIMER_PAIR_EXPLAIN=considered 11, high end compl 10, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=24308.407718 PRIMER_LEFT_0_PENALTY=302.282278 PRIMER_RIGHT_0_PENALTY=24006.125439 PRIMER_LEFT_0_SEQUENCE=TGAGATCTGGAGGATCCAAG PRIMER_RIGHT_0_SEQUENCE=TCTTCAAACTTTGGACATTCATAA PRIMER_LEFT_0=39,20 PRIMER_RIGHT_0=237,24 PRIMER_LEFT_0_TM=57.718 PRIMER_RIGHT_0_TM=57.875 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=29.167 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=300.000000 PRIMER_RIGHT_0_POSITION_PENALTY=24000.000000 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=6.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=199 = SEQUENCE_ID=HMGCR_X2 SEQUENCE_TARGET=0,112 SEQUENCE_TEMPLATE=GGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGCATAGCCATCCTGTATATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTG PRIMER_LEFT_EXPLAIN=considered 770, GC content failed 5, low tm 460, high tm 81, ok 224 PRIMER_RIGHT_EXPLAIN=considered 770, GC content failed 11, low tm 448, high tm 81, high any compl 7, high end compl 1, ok 222 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=49011.420343 PRIMER_LEFT_0_PENALTY=20002.797253 PRIMER_RIGHT_0_PENALTY=29008.623090 PRIMER_LEFT_0_SEQUENCE=GGATGTTTTGAGCAGTGACA PRIMER_RIGHT_0_SEQUENCE=AATATATTTTGATCCAAGTTGACGTA PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=108,26 PRIMER_LEFT_0_TM=57.203 PRIMER_RIGHT_0_TM=57.377 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=26.923 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=20000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=29000.000000 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=109 = SEQUENCE_ID=HMGCR_X3 SEQUENCE_TARGET=0,88 SEQUENCE_TEMPLATE=GGTATTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCACTTCTTAGACAAAGAATTGACAGGCTTGA PRIMER_LEFT_EXPLAIN=considered 530, GC content failed 1, low tm 361, high tm 44, ok 124 PRIMER_RIGHT_EXPLAIN=considered 530, GC content failed 1, low tm 416, high tm 4, ok 109 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=40005.823174 PRIMER_LEFT_0_PENALTY=19002.290742 PRIMER_RIGHT_0_PENALTY=21003.532432 PRIMER_LEFT_0_SEQUENCE=GGTATTGCTGGCCTTTTCA PRIMER_RIGHT_0_SEQUENCE=TCAAGCCTGTCAATTCTTTGT PRIMER_LEFT_0=0,19 PRIMER_RIGHT_0=87,21 PRIMER_LEFT_0_TM=58.709 PRIMER_RIGHT_0_TM=57.468 PRIMER_LEFT_0_GC_PERCENT=47.368 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=19000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=21000.000000 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 = SEQUENCE_ID=HMGCR_X4 SEQUENCE_TARGET=0,85 SEQUENCE_TEMPLATE=ATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTAGCAAAGTTTGCCCTCAGTTCCAACTCACA PRIMER_LEFT_EXPLAIN=considered 500, low tm 151, high tm 175, ok 174 PRIMER_RIGHT_EXPLAIN=considered 500, low tm 123, high tm 202, ok 175 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=39005.482012 PRIMER_LEFT_0_PENALTY=20001.557050 PRIMER_RIGHT_0_PENALTY=19003.924962 PRIMER_LEFT_0_SEQUENCE=ATGAAGCTTTGCCCTTTTTC PRIMER_RIGHT_0_SEQUENCE=TGTGAGTTGGAACTGAGGG PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=84,19 PRIMER_LEFT_0_TM=58.443 PRIMER_RIGHT_0_TM=57.075 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=52.632 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=20000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=19000.000000 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=85 = SEQUENCE_ID=HMGCR_X5 SEQUENCE_TARGET=0,106 SEQUENCE_TEMPLATE=GGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTTTAGGTCCTACGTTTACCCTCGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCA PRIMER_LEFT_EXPLAIN=considered 710, low tm 212, high tm 246, high end compl 1, ok 251 PRIMER_RIGHT_EXPLAIN=considered 710, low tm 202, high tm 248, high end compl 1, high repeat similarity 1, ok 258 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=47007.389548 PRIMER_LEFT_0_PENALTY=25005.106680 PRIMER_RIGHT_0_PENALTY=22002.282868 PRIMER_LEFT_0_SEQUENCE=GGATGAAGTAAGGGAAAATATTGCT PRIMER_RIGHT_0_SEQUENCE=TGACATGGTACCAACTCCAATC PRIMER_LEFT_0=0,25 PRIMER_RIGHT_0=105,22 PRIMER_LEFT_0_TM=60.107 PRIMER_RIGHT_0_TM=59.717 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=25000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=22000.000000 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=106 = SEQUENCE_ID=HMGCR_X6 SEQUENCE_TARGET=0,107 SEQUENCE_TEMPLATE=GGGGTACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTACTTCGTGTTCATGACTTTCTTCCCAGCTTGTGTGTCCTTGGTATTAGA PRIMER_LEFT_EXPLAIN=considered 720, low tm 206, high tm 309, ok 205 PRIMER_RIGHT_EXPLAIN=considered 720, low tm 181, high tm 338, ok 201 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=41009.536490 PRIMER_LEFT_0_PENALTY=18004.862764 PRIMER_RIGHT_0_PENALTY=23004.673725 PRIMER_LEFT_0_SEQUENCE=GGGGTACGTCAGCTTGAA PRIMER_RIGHT_0_SEQUENCE=TCTAATACCAAGGACACACAAGC PRIMER_LEFT_0=0,18 PRIMER_RIGHT_0=106,23 PRIMER_LEFT_0_TM=57.137 PRIMER_RIGHT_0_TM=58.326 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_RIGHT_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=18000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=23000.000000 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=107 = SEQUENCE_ID=HMGCR_X7 SEQUENCE_TARGET=0,117 SEQUENCE_TEMPLATE=GCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGCAGCTCAGCCATTTTGCCCGAGTTTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAAGATGATTAT PRIMER_LEFT_EXPLAIN=considered 820, GC content failed 3, low tm 162, high tm 464, ok 191 PRIMER_RIGHT_EXPLAIN=considered 820, GC content failed 3, low tm 237, high tm 349, ok 231 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=59009.628979 PRIMER_LEFT_0_PENALTY=35003.015981 PRIMER_RIGHT_0_PENALTY=24006.612999 PRIMER_LEFT_0_SEQUENCE=GCGAGGGTCGTCCAATTT PRIMER_RIGHT_0_SEQUENCE=ATAATCATCTTGACCCTCTGAGTT PRIMER_LEFT_0=17,18 PRIMER_RIGHT_0=116,24 PRIMER_LEFT_0_TM=61.016 PRIMER_RIGHT_0_TM=57.387 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=35000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=24000.000000 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 = SEQUENCE_ID=HMGCR_X8 SEQUENCE_TARGET=0,161 SEQUENCE_TEMPLATE=GTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCAAAACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAGAATTGAACCAAGTGTTTCCCTCTGGCAGTTTTATCTCTCTA PRIMER_LEFT_EXPLAIN=considered 1260, low tm 540, high tm 321, ok 399 PRIMER_RIGHT_EXPLAIN=considered 1260, low tm 530, high tm 315, high repeat similarity 20, ok 395 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=51007.112761 PRIMER_LEFT_0_PENALTY=23004.249004 PRIMER_RIGHT_0_PENALTY=28002.863756 PRIMER_LEFT_0_SEQUENCE=TCTCTAGGCTTGGTTCTTGTTC PRIMER_RIGHT_0_SEQUENCE=GATAAAACTGCCAGAGGGAAAC PRIMER_LEFT_0=1,22 PRIMER_RIGHT_0=154,22 PRIMER_LEFT_0_TM=57.751 PRIMER_RIGHT_0_TM=59.136 PRIMER_LEFT_0_GC_PERCENT=45.455 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=23000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=28000.000000 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=154 = SEQUENCE_ID=HMGCR_X9 SEQUENCE_TARGET=0,248 SEQUENCE_TEMPLATE=AAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAGTTTAGCTCTCCTTCTGGCTGTCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTAAAAAACCCTATCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTATGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAGAAAGAAAA PRIMER_LEFT_EXPLAIN=considered 1977, GC content failed 1, low tm 1154, high tm 176, high end compl 1, long poly-x seq 22, ok 623 PRIMER_RIGHT_EXPLAIN=considered 1977, low tm 1125, high tm 203, long poly-x seq 22, ok 627 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=44008.165484 PRIMER_LEFT_0_PENALTY=23004.538081 PRIMER_RIGHT_0_PENALTY=21003.627403 PRIMER_LEFT_0_SEQUENCE=AAATGATCAGCATGGATATTGAA PRIMER_RIGHT_0_SEQUENCE=TTTTCTTTCTCGGTTTATCCC PRIMER_LEFT_0=0,23 PRIMER_RIGHT_0=247,21 PRIMER_LEFT_0_TM=58.462 PRIMER_RIGHT_0_TM=57.373 PRIMER_LEFT_0_GC_PERCENT=30.435 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=23000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=21000.000000 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=9.3000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=248 = SEQUENCE_ID=HMGCR_X10 SEQUENCE_TARGET=0,90 SEQUENCE_TEMPLATE=GTTGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTGGTTGGTAACTCCTCCTTACTCGATACTTCA PRIMER_LEFT_EXPLAIN=considered 550, low tm 222, high tm 109, ok 219 PRIMER_RIGHT_EXPLAIN=considered 550, low tm 227, high tm 116, ok 207 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=49012.458140 PRIMER_LEFT_0_PENALTY=25006.169796 PRIMER_RIGHT_0_PENALTY=24006.288343 PRIMER_LEFT_0_SEQUENCE=TTGAGGTTATAAAACCCTTAGTGG PRIMER_RIGHT_0_SEQUENCE=TGAAGTATCGAGTAAGGAGGAGTT PRIMER_LEFT_0=1,24 PRIMER_RIGHT_0=89,24 PRIMER_LEFT_0_TM=57.830 PRIMER_RIGHT_0_TM=57.712 PRIMER_LEFT_0_GC_PERCENT=37.500 PRIMER_RIGHT_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=25000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=24000.000000 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=89 = SEQUENCE_ID=HMGCR_X11 SEQUENCE_TARGET=0,89 SEQUENCE_TEMPLATE=TCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGCCTAATGAAGAATGTCTACAGATACTTGGGAATGCAGA PRIMER_LEFT_EXPLAIN=considered 540, low tm 135, high tm 249, high end compl 1, ok 155 PRIMER_RIGHT_EXPLAIN=considered 540, low tm 181, high tm 215, ok 144 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=44006.176345 PRIMER_LEFT_0_PENALTY=23002.457721 PRIMER_RIGHT_0_PENALTY=21003.718624 PRIMER_LEFT_0_SEQUENCE=CAGTACTGGTGACACAGGAACC PRIMER_RIGHT_0_SEQUENCE=TCTGCATTCCCAAGTATCTGT PRIMER_LEFT_0=1,22 PRIMER_RIGHT_0=88,21 PRIMER_LEFT_0_TM=59.542 PRIMER_RIGHT_0_TM=57.281 PRIMER_LEFT_0_GC_PERCENT=54.545 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=23000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=21000.000000 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 = SEQUENCE_ID=HMGCR_X12 SEQUENCE_TARGET=0,195 SEQUENCE_TEMPLATE=GAAAGGTGCAAAATTCCTTAGTGATGCTGAGATCATCCAGTTAGTCAATGCTAAGCATATCCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGTGGTGTATCTATTCGCCGACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTTACAGGGATTATAATTACTCCTT PRIMER_LEFT_EXPLAIN=considered 1600, low tm 646, high tm 347, ok 607 PRIMER_RIGHT_EXPLAIN=considered 1600, low tm 707, high tm 324, ok 569 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=50013.286753 PRIMER_LEFT_0_PENALTY=23003.785045 PRIMER_RIGHT_0_PENALTY=27009.501708 PRIMER_LEFT_0_SEQUENCE=GAAAGGTGCAAAATTCCTTAGTG PRIMER_RIGHT_0_SEQUENCE=AAGGAGTAATTATAATCCCTGTAAGGT PRIMER_LEFT_0=0,23 PRIMER_RIGHT_0=194,27 PRIMER_LEFT_0_TM=59.215 PRIMER_RIGHT_0_TM=57.498 PRIMER_LEFT_0_GC_PERCENT=39.130 PRIMER_RIGHT_0_GC_PERCENT=33.333 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=23000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=27000.000000 PRIMER_LEFT_0_END_STABILITY=5.7000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=195 = SEQUENCE_ID=HMGCR_X13 SEQUENCE_TARGET=0,159 SEQUENCE_TEMPLATE=GGTGATGGGAGCTTGTTGTGAGAATGTTATTGGATATATGCCCATCCCTGTTGGAGTGGCAGGACCCCTTTGCTTAGATGAAAAAGAATTTCAGGTTCCAATGGCAACAACAGAAGGTTGTCTTGTGGCCAGCACCAATAGAGGCTGCAGAGCAATAGG PRIMER_LEFT_EXPLAIN=considered 1240, GC content failed 1, low tm 314, high tm 591, ok 334 PRIMER_RIGHT_EXPLAIN=considered 1240, GC content failed 1, low tm 280, high tm 628, ok 331 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=37006.814000 PRIMER_LEFT_0_PENALTY=18004.525596 PRIMER_RIGHT_0_PENALTY=19002.288404 PRIMER_LEFT_0_SEQUENCE=GGTGATGGGAGCTTGTTG PRIMER_RIGHT_0_SEQUENCE=CCTATTGCTCTGCAGCCTC PRIMER_LEFT_0=0,18 PRIMER_RIGHT_0=158,19 PRIMER_LEFT_0_TM=57.474 PRIMER_RIGHT_0_TM=58.712 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=18000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=19000.000000 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=159 = SEQUENCE_ID=HMGCR_X14 SEQUENCE_TARGET=0,158 SEQUENCE_TEMPLATE=TCTTGGTGGAGGTGCCAGCAGCCGAGTCCTTGCAGATGGGATGACTCGTGGCCCAGTTGTGCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGCCTGGCTCGAAACATCTGAAGGGTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCA PRIMER_LEFT_EXPLAIN=considered 1230, low tm 83, high tm 898, ok 249 PRIMER_RIGHT_EXPLAIN=considered 1230, low tm 122, high tm 805, ok 303 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=38002.854310 PRIMER_LEFT_0_PENALTY=18002.835611 PRIMER_RIGHT_0_PENALTY=20000.018700 PRIMER_LEFT_0_SEQUENCE=TCTTGGTGGAGGTGCCAG PRIMER_RIGHT_0_SEQUENCE=TGCTAGTGCTGTCAAATGCC PRIMER_LEFT_0=0,18 PRIMER_RIGHT_0=157,20 PRIMER_LEFT_0_TM=60.836 PRIMER_RIGHT_0_TM=60.019 PRIMER_LEFT_0_GC_PERCENT=61.111 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=18000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=20000.000000 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=9.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=158 = SEQUENCE_ID=HMGCR_X15 SEQUENCE_TARGET=0,106 SEQUENCE_TEMPLATE=GATTTGCACGTCTACAGAAACTTCATACAAGTATAGCTGGACGCAACCTTTATATCCGTTTCCAGTCCAGGTCAGGGGATGCCATGGGGATGAACATGATTTCAAA PRIMER_LEFT_EXPLAIN=considered 710, low tm 257, high tm 250, ok 203 PRIMER_RIGHT_EXPLAIN=considered 710, low tm 172, high tm 343, ok 195 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=44006.746041 PRIMER_LEFT_0_PENALTY=23005.359396 PRIMER_RIGHT_0_PENALTY=21001.386646 PRIMER_LEFT_0_SEQUENCE=GATTTGCACGTCTACAGAAACTT PRIMER_RIGHT_0_SEQUENCE=TTTGAAATCATGTTCATCCCC PRIMER_LEFT_0=0,23 PRIMER_RIGHT_0=105,21 PRIMER_LEFT_0_TM=57.641 PRIMER_RIGHT_0_TM=59.613 PRIMER_LEFT_0_GC_PERCENT=39.130 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=23000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=21000.000000 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=10.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=106 = SEQUENCE_ID=HMGCR_X16 SEQUENCE_TARGET=0,171 SEQUENCE_TEMPLATE=GGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGAAATGCAGATTCTAGCCGTTAGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAGGGAAGAGGAAAATCTGTTGTTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGA PRIMER_LEFT_EXPLAIN=considered 1360, low tm 684, high tm 181, high end compl 8, high repeat similarity 2, ok 485 PRIMER_RIGHT_EXPLAIN=considered 1360, low tm 616, high tm 248, high end compl 1, ok 495 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=48007.297123 PRIMER_LEFT_0_PENALTY=28005.907183 PRIMER_RIGHT_0_PENALTY=20001.389940 PRIMER_LEFT_0_SEQUENCE=CAGAGAAAGCACTTTCAAAACTT PRIMER_RIGHT_0_SEQUENCE=TCTCTGACAACCTTGGCTGG PRIMER_LEFT_0=5,23 PRIMER_RIGHT_0=170,20 PRIMER_LEFT_0_TM=57.093 PRIMER_RIGHT_0_TM=61.390 PRIMER_LEFT_0_GC_PERCENT=34.783 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=28000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=20000.000000 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=166 = SEQUENCE_ID=HMGCR_X17 SEQUENCE_TARGET=0,141 SEQUENCE_TEMPLATE=AGTATTAAAGACTACCACAGAGGCTATGATTGAGGTCAACATTAACAAGAATTTAGTGGGCTCTGCCATGGCTGGGAGCATAGGAGGCTACAACGCCCATGCAGCAAACATTGTCACCGCCATCTACATTGCCTGTGGACA PRIMER_LEFT_EXPLAIN=considered 1060, GC content failed 1, low tm 295, high tm 528, high end compl 3, ok 233 PRIMER_RIGHT_EXPLAIN=considered 1060, GC content failed 1, low tm 217, high tm 601, ok 241 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=50007.199789 PRIMER_LEFT_0_PENALTY=29005.780871 PRIMER_RIGHT_0_PENALTY=21001.418918 PRIMER_LEFT_0_SEQUENCE=AAAGACTACCACAGAGGCTATGA PRIMER_RIGHT_0_SEQUENCE=TGTCCACAGGCAATGTAGATG PRIMER_LEFT_0=6,23 PRIMER_RIGHT_0=140,21 PRIMER_LEFT_0_TM=57.219 PRIMER_RIGHT_0_TM=59.581 PRIMER_LEFT_0_GC_PERCENT=43.478 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=29000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=21000.000000 PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=135 = SEQUENCE_ID=HMGCR_X18 SEQUENCE_TARGET=0,159 SEQUENCE_TEMPLATE=GGATGCAGCACAGAATGTTGGTAGTTCAAACTGTATTACTTTAATGGAAGCAAGTGGTCCCACAAATGAAGATTTATATATCAGCTGCACCATGCCATCTATAGAGATAGGAACGGTGGGTGGTGGGACCAACCTACTACCTCAGCAAGCCTGTTTGCA PRIMER_LEFT_EXPLAIN=considered 1240, GC content failed 18, low tm 522, high tm 395, high repeat similarity 2, ok 303 PRIMER_RIGHT_EXPLAIN=considered 1240, GC content failed 18, low tm 504, high tm 411, ok 307 PRIMER_PAIR_EXPLAIN=considered 62, high end compl 59, ok 3 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=40003.344317 PRIMER_LEFT_0_PENALTY=20000.272067 PRIMER_RIGHT_0_PENALTY=20003.072251 PRIMER_LEFT_0_SEQUENCE=GGATGCAGCACAGAATGTTG PRIMER_RIGHT_0_SEQUENCE=GCAAACAGGCTTGCTGAGG PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=157,19 PRIMER_LEFT_0_TM=60.272 PRIMER_RIGHT_0_TM=62.072 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=20000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=20000.000000 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=158 = SEQUENCE_ID=HMGCR_X19 SEQUENCE_TARGET=0,155 SEQUENCE_TEMPLATE=GATGCTAGGTGTTCAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGCCCGAATTGTGTGTGGGACCGTAATGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGCAGGACATCTTGTCAAAAGTCACATGATTCACAACA PRIMER_LEFT_EXPLAIN=considered 1200, low tm 119, high tm 823, ok 258 PRIMER_RIGHT_EXPLAIN=considered 1200, low tm 167, high tm 784, ok 249 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=44006.457265 PRIMER_LEFT_0_PENALTY=21002.049610 PRIMER_RIGHT_0_PENALTY=23004.407655 PRIMER_LEFT_0_SEQUENCE=GATGCTAGGTGTTCAAGGAGC PRIMER_RIGHT_0_SEQUENCE=TGTTGTGAATCATGTGACTTTTG PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=154,23 PRIMER_LEFT_0_TM=58.950 PRIMER_RIGHT_0_TM=58.592 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=21000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=23000.000000 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=155 = SEQUENCE_ID=HMGCR_X20 SEQUENCE_TARGET=0,56 SEQUENCE_TEMPLATE=GGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGAATAGCCCGACAGTTCTGAACTGGAACATGGGCATTGGGTTCTAAAGGACTAACATAAAATCTGTGAATTAAAAAAGCTCAATGCATTGTCTTGTGGAGGATGAATAAA PRIMER_LEFT_EXPLAIN=considered 444, overlap target 99, low tm 91, high tm 152, ok 102 PRIMER_RIGHT_EXPLAIN=considered 1150, GC content failed 42, low tm 311, high tm 512, high any compl 26, high end compl 3, long poly-x seq 20, ok 236 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=24265.616387 PRIMER_LEFT_0_PENALTY=24004.237346 PRIMER_RIGHT_0_PENALTY=261.379041 PRIMER_LEFT_0_SEQUENCE=GGTCGAAGATCAATTTACAAGACC PRIMER_RIGHT_0_SEQUENCE=GCCCATGTTCCAGTTCAGA PRIMER_LEFT_0=0,24 PRIMER_RIGHT_0=87,19 PRIMER_LEFT_0_TM=60.237 PRIMER_RIGHT_0_TM=59.621 PRIMER_LEFT_0_GC_PERCENT=41.667 PRIMER_RIGHT_0_GC_PERCENT=52.632 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_POSITION_PENALTY=24000.000000 PRIMER_RIGHT_0_POSITION_PENALTY=260.000000 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=88 = primer3-2.3.6/test/thal_test.pl0000775000077300007730000002300312231156245015362 0ustar ioanaioana # Usage: perl thal_test.pl [ --valgrind ] # This is the driver for tests of thal (thal.{c,h}, using in the wrapper # C program ntthal (coded in ntthal_main.c) # ====================================================================== # (c) Copyright 1996,1997,1998,1999,2000,2001,2004,2006,2007, 2008, 2009 # Whitehead Institute for Biomedical Research, Steve Rozen, # Andreas Untergasser and Helen Skaletsky # All rights reserved. # # This file is part of the primer3 suite and the thal library. # # The primer3 suite is free software; you can # redistribute it and/or modify it under the terms of the GNU # General Public License as published by the Free Software Foundation; # either version 2 of the License, or (at your option) any later # version. # # This software is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # # You should have received a copy of the GNU General Public License # along with this file (file gpl-2.0.txt in the source distribution); if # not, write to the Free Software Foundation, Inc., 51 Franklin St, # Fifth Floor, Boston, MA 02110-1301 USA # # THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS # "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT # LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR # A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT # OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, # SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT # LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, # DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY # THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT # (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE # OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. # ====================================================================== use strict; use warnings 'all'; use Getopt::Long; use Carp; use POSIX; # For testing wait / system return value, e.g. WIFSIGNALED, WTERMSIG..... use Config; # To get the number for SIGINT our $test_count = 0; our $exit_status = 0; our $do_valgrind; our %signo; our $valgrind_exe = "/usr/local/bin/valgrind"; our $valgrind_format; our $winFlag; our $exe = '../src/ntthal'; sub perldiff($$); sub main(); # Call system() with warnings turned off; needed for ActiveState / MS Windows. sub _nowarn_system($); main(); sub runtest($$$$$) { my ($desc, $ntthal_args, $infile, $benchfile, $outfile) = @_; print $desc, '...'; open Q, $infile or confess "open $infile: $!"; my $errfile = "$outfile.err"; # Reopen STDOUT to get all ntthal output in one file open OLDOUT, ">&STDOUT" or confess "Cannot dup STDOUT: $!"; open OLDERR, ">&" , \*STDERR or confess "Cannot dup STDERR: $!"; open STDOUT, '>', $outfile or confess "open STDOUT '>' $outfile: $!"; open STDERR, '>', $errfile or confess "open STDERR '>' $errfile $!"; while (my $in = ) { $test_count++; my $valgrind_prefix = $do_valgrind ? sprintf($valgrind_format, $test_count) : ''; my $cmd = "$valgrind_prefix $exe $ntthal_args $in"; my $r = _nowarn_system($cmd); } close Q; open STDOUT, ">&OLDOUT" or confess "Cannot dup OLDOUT: $!"; open STDERR, ">&OLDERR" or confess "Cannot dup OLDERR: $!"; close OLDERR; close OLDOUT; my $r = perldiff $benchfile, $outfile; if ($r == 0) { print "[OK]\n"; } else { print "[FAILED]\n"; $exit_status = -1; } } sub main() { my %args; # select STDERR; $| = 1; if (!GetOptions(\%args, 'valgrind', 'windows', )) { print "Usage: $0 [ --valgrind ] [ --windows ]\n"; exit -1; } $winFlag = defined $args{'windows'}; $do_valgrind = $args{'valgrind'}; if ($winFlag && $do_valgrind) { print "$0: Cannot specify both --valgrind and --windows\n"; exit -1; } if ((!-x $valgrind_exe) && ($do_valgrind)) { warn "Cannot find $valgrind_exe; will try `which valgrind`\n"; $valgrind_exe= `which valgrind`; chomp($valgrind_exe); if (!$valgrind_exe || ! -x $valgrind_exe) { die "Cannot execute $valgrind_exe"; } } $valgrind_format = "$valgrind_exe --leak-check=yes " . " --show-reachable=yes --log-file=ntthal.%0.4d.valg "; if ($winFlag) { $exe = '..\\src\\ntthal.exe'; } # Look for the ntthal executable die "Cannot execute $exe" unless -x $exe; my $valgrind_prefix = $do_valgrind ? sprintf($valgrind_format, $test_count) : ''; # ================================================== # First test # Test error handling on over-long input sequence: print "Error handling of too-long sequence...\n"; my @foo=(); my $cmd = "$valgrind_prefix $exe -s1 ACGTGTTCGTCGTAGACGTGTTCGTCGTAGACGTGTTCGTCGTAGACGTGTTCGTCGTAGTG -s2 ACGTGTTCGTCGTATGACGTGTTCGTCGTAGACGTGTTCGTCGTAGACGTGTTCGTCGTAG -a ANY > thal.tmp 2>&1"; my $r = _nowarn_system($cmd); open X, 'thal.tmp'; # Get the test output @foo = ; # Snarf it close X; # Check the output..... if ($foo[0] eq "Error: Both sequences longer than 60 for thermodynamic alignment\n") { print "1................................... [OK]\n" } else { print "$foo[0]\n1.................................. [FAILED]\n"; $exit_status = -1; } unlink("thal.tmp"); # ================================================== # Additional tests using runtest() runtest('Default implementations + alignment', "", "thal_input", 'thal_output', 'thal_output.tmp'); runtest('Default implementations + NO alignment 1', "-r", 'thal_input', 'thal_temp_output', 'thal_temp_output.tmp'); runtest('Default implementations + NO alignment 2', "-r", "thal_long_input", 'thal_long_temp_output', "thal_long_temp_output.tmp"); # ================================================== # If we were running under valgrind to look for memory-related # errors (reading uninitialized memory, writing off the end of # an array, etc) or leaks, then we look through the valgrind # logs to summarize errors and leaks. if ($do_valgrind) { # Assume this is Unix/Linux envrionment, so # we have grep. my $r = system "grep ERROR ntthal.*.*valg* | grep -v 'ERROR SUMMARY: 0 errors'"; if (!$r) { # !$r because grep returns 0 if something is found, # and if something is found, we have a problem. $exit_status = -1; } $r = system "grep 'definitely lost' ntthal.*.*valg* | grep -v '0 bytes'"; if (!$r) { $exit_status = -1; } $r = system "grep 'possibly lost' ntthal.*.*valg* | grep -v '0 bytes'"; if (!$r) { $exit_status = -1; } } exit $exit_status; } sub round_numbers($) { my ($line) = @_; my ($i, $t1, $t2); my $new = ""; chomp($line); my @fields = split("\t", $line); for $i (0..$#fields) { if ($fields[$i] =~ /([a-zA-Z0-9]\s*=\s*-*)([0-9.]+)/) { $t1 = $1; $t2 = $2; $t2 = sprintf "%0.*f", 1, $t2; $fields[$i] = $t1.$t2; } } $new = join("\t", @fields); return $new; } # Usage: perldiff("filename1", "filename2") # Return 0 if no differences are found, # othewise return 1; sub perldiff($$) { my ($f1, $f2) = @_; open F1, $f1 or die "open $f1: $!"; open F2, $f2 or die "open $f2: $!"; my @f1 = ; my @f2 = ; # If different number of lines, return FAIL. if (@f1 != @f2) { print "Different number of lines\n"; return 1; } # check for differences on the lines, themselves my $linenumber = 0; my $line_end_diff = 0; # iterate using lines in file1 while (@f1) { $linenumber++; # get the lines from each respective file my $l1 = shift @f1; my $l2 = shift @f2; my $l1_orig = $l1; my $l2_orig = $l2; $linenumber++; # Check for difference between two edited lines (line by line) # special case for lines with thermodynamical parameters values (round to 1 decimal) if ($l1 =~ /Calculated thermodynamical parameters for dimer/) { my $new_l1 = round_numbers($l1); my $new_l2 = round_numbers($l2); if ($new_l1 ne $new_l2) { print "Difference found at line $linenumber:\n< $l1_orig\n> $l2_orig\n"; return 1; } } elsif ($l1 ne $l2) { print "Difference found at line $linenumber:\n< $l1_orig\n> $l2_orig\n"; return 1; } } return 0; } sub _nowarn_system($) { my $cmd = shift; no warnings 'all'; my $r = system $cmd; my $r2 = $?; if (!$winFlag && WIFEXITED($r2)) { $r = WEXITSTATUS($r2); if (defined $signo{'INT'}) { if ($r == $signo{'INT'}) { print "\nCommand: $cmd\n"; print "generated exit value $r\n"; print "Presumably caused by catching SIGINT\n"; print "Tests halted\n\n"; print "\nWARNING: not all tests executed ... [FAILED]\n"; exit; } } } if (!$winFlag && WIFSIGNALED($r2)) { my $r2 = WTERMSIG($r2); print "Exited with signal $r2\n"; } $r; } primer3-2.3.6/test/thal_long_temp_output0000775000077300007730000000013512231156245017376 0ustar ioanaioana13.133253 20.418964 20.418964 -20.586915 21.468295 21.468295 -17.437722 13.133253 -22.824122 primer3-2.3.6/test/primer_must_use_th_output0000664000077300007730000005502112231156245020320 0ustar ioanaioanaPRIMER_DNTP_CONC=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 SEQUENCE_ID=anyway_1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 PRIMER_PICK_ANYWAY=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, high hairpin stability 3, ok 0 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=high_tm PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.436785 PRIMER_LEFT_0_PENALTY=2.141348 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=58.859 PRIMER_RIGHT_0_TM=59.705 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY_TH=33.86 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_LEFT_0_SELF_END_TH=13.96 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=50.77 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=high_tm_and_self_any PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.436785 PRIMER_LEFT_0_PENALTY=2.141348 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=58.859 PRIMER_RIGHT_0_TM=59.705 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY_TH=33.86 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_LEFT_0_SELF_END_TH=13.96 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=50.77 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=high_tm_and_self_any_and_target PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=20,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_RIGHT_EXPLAIN=considered 3, high tm 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.436785 PRIMER_LEFT_0_PENALTY=2.141348 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_LEFT_0_PROBLEMS= Overlaps target; Hairpin stability too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=58.859 PRIMER_RIGHT_0_TM=59.705 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY_TH=33.86 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_LEFT_0_SELF_END_TH=13.96 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=50.77 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=high_tm_and_self_any_and_excl_region PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 SEQUENCE_EXCLUDED_REGION=20,1 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.436785 PRIMER_LEFT_0_PENALTY=2.141348 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_LEFT_0_PROBLEMS= Overlaps an excluded region; Hairpin stability too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=58.859 PRIMER_RIGHT_0_TM=59.705 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY_TH=33.86 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_LEFT_0_SELF_END_TH=13.96 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=50.77 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=high_tm_and_self_any_and_incl_region PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 SEQUENCE_INCLUDED_REGION=1,160 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_ERROR=Specified left primer not in Included Region = SEQUENCE_ID=left_high_tm_and_self_any_and_right PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.436785 PRIMER_LEFT_0_PENALTY=2.141348 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=58.859 PRIMER_RIGHT_0_TM=59.705 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY_TH=33.86 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_LEFT_0_SELF_END_TH=13.96 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=50.77 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.436785 PRIMER_LEFT_0_PENALTY=2.141348 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high; PRIMER_RIGHT_0_PROBLEMS= GC content too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=58.859 PRIMER_RIGHT_0_TM=59.705 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY_TH=33.86 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_LEFT_0_SELF_END_TH=13.96 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=50.77 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc_and_target PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.436785 PRIMER_LEFT_0_PENALTY=2.141348 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high; PRIMER_RIGHT_0_PROBLEMS= Overlaps target; GC content too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=58.859 PRIMER_RIGHT_0_TM=59.705 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY_TH=33.86 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_LEFT_0_SELF_END_TH=13.96 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=50.77 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=internal_too_short PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccat PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 PRIMER_WARNING=Specified internal oligo < PRIMER_INTERNAL_MIN_SIZE; PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.436785 PRIMER_LEFT_0_PENALTY=2.141348 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_INTERNAL_0_PENALTY=28.729043 PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high; PRIMER_RIGHT_0_PROBLEMS= Overlaps target; GC content too high; PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; Too short; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_0_SEQUENCE=gcgtacgatccat PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_INTERNAL_0=23,13 PRIMER_LEFT_0_TM=58.859 PRIMER_RIGHT_0_TM=59.705 PRIMER_INTERNAL_0_TM=38.271 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=53.846 PRIMER_INTERNAL_0_SELF_ANY_TH=18.49 PRIMER_LEFT_0_SELF_ANY_TH=33.86 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=13.96 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=50.77 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=internal_ok PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.436785 PRIMER_LEFT_0_PENALTY=2.141348 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_INTERNAL_0_PENALTY=7.995085 PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high; PRIMER_RIGHT_0_PROBLEMS= Overlaps target; GC content too high; PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_0_SEQUENCE=gcgtacgatccatgctagc PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_INTERNAL_0=23,19 PRIMER_LEFT_0_TM=58.859 PRIMER_RIGHT_0_TM=59.705 PRIMER_INTERNAL_0_TM=53.005 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=57.895 PRIMER_INTERNAL_0_SELF_ANY_TH=18.49 PRIMER_LEFT_0_SELF_ANY_TH=33.86 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_INTERNAL_0_SELF_END_TH=4.81 PRIMER_LEFT_0_SELF_END_TH=13.96 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=50.77 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=internal_too_cold PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.436785 PRIMER_LEFT_0_PENALTY=2.141348 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_INTERNAL_0_PENALTY=7.995085 PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high; PRIMER_RIGHT_0_PROBLEMS= Overlaps target; GC content too high; PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_0_SEQUENCE=gcgtacgatccatgctagc PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_INTERNAL_0=23,19 PRIMER_LEFT_0_TM=58.859 PRIMER_RIGHT_0_TM=59.705 PRIMER_INTERNAL_0_TM=53.005 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=57.895 PRIMER_INTERNAL_0_SELF_ANY_TH=18.49 PRIMER_LEFT_0_SELF_ANY_TH=33.86 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_INTERNAL_0_SELF_END_TH=4.81 PRIMER_LEFT_0_SELF_END_TH=13.96 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=50.77 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=internal_too_cold2 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=CCTACAGCTGTGGGAAAATG SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=100 PRIMER_MAX_GC=100 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.984574 PRIMER_LEFT_0_PENALTY=2.689137 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_INTERNAL_0_PENALTY=7.995085 PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high; PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; PRIMER_LEFT_0_SEQUENCE=CCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_0_SEQUENCE=gcgtacgatccatgctagc PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=160,18 PRIMER_INTERNAL_0=23,19 PRIMER_LEFT_0_TM=57.311 PRIMER_RIGHT_0_TM=59.705 PRIMER_INTERNAL_0_TM=53.005 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=57.895 PRIMER_INTERNAL_0_SELF_ANY_TH=18.49 PRIMER_LEFT_0_SELF_ANY_TH=29.57 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_INTERNAL_0_SELF_END_TH=4.81 PRIMER_LEFT_0_SELF_END_TH=8.02 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=48.92 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=160 = SEQUENCE_ID=mispriming PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=CCTACAGCTGTGGGAAAATG SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=100 PRIMER_MAX_GC=100 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_MAX_LIBRARY_MISPRIMING=8 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.984574 PRIMER_LEFT_0_PENALTY=2.689137 PRIMER_RIGHT_0_PENALTY=2.295437 PRIMER_INTERNAL_0_PENALTY=7.995085 PRIMER_LEFT_0_PROBLEMS= Hairpin stability too high; Similarity to non-template sequence too high; PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; PRIMER_LEFT_0_SEQUENCE=CCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_0_SEQUENCE=gcgtacgatccatgctagc PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=160,18 PRIMER_INTERNAL_0=23,19 PRIMER_LEFT_0_TM=57.311 PRIMER_RIGHT_0_TM=59.705 PRIMER_INTERNAL_0_TM=53.005 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=57.895 PRIMER_INTERNAL_0_SELF_ANY_TH=18.49 PRIMER_LEFT_0_SELF_ANY_TH=29.57 PRIMER_RIGHT_0_SELF_ANY_TH=0.27 PRIMER_INTERNAL_0_SELF_END_TH=4.81 PRIMER_LEFT_0_SELF_END_TH=8.02 PRIMER_RIGHT_0_SELF_END_TH=0.27 PRIMER_LEFT_0_HAIRPIN_TH=48.92 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=2.3200 PRIMER_RIGHT_0_END_STABILITY=6.5300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=160 = primer3-2.3.6/test/primer_mispriming_boundary1_input0000664000077300007730000000270712231156245021725 0ustar ioanaioanaSEQUENCE_ID=pair_1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY=primer_repeat_boundary1_lib PRIMER_INTERNAL_MISHYB_LIBRARY=primer_repeat_boundary1_lib SEQUENCE_TEMPLATE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_LIBRARY_MISPRIMING=0 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=0 PRIMER_MAX_SELF_ANY=25 PRIMER_MAX_SELF_END=25 PRIMER_PAIR_MAX_COMPL_ANY=25 PRIMER_PAIR_MAX_COMPL_END=25 PRIMER_INTERNAL_MAX_SELF_ANY=25 PRIMER_INTERNAL_MAX_SELF_END=25 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_SIZE=20 PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_INTERNAL_MAX_GC=100 PRIMER_MAX_SIZE=20 PRIMER_MIN_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MAX_TM=100 PRIMER_MIN_TM=0 PRIMER_OPT_TM=60 = SEQUENCE_ID=pair_0 P3_COMMENT="Should fail because of high repeat similarity" SEQUENCE_TEMPLATE=TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA = SEQUENCE_ID=pair_1 SEQUENCE_TEMPLATE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=0 PRIMER_MAX_LIBRARY_MISPRIMING=20 = primer3-2.3.6/test/primer_must_use_formatted_output0000664000077300007730000004300712231156245021673 0ustar ioanaioanaPRIMER PICKING RESULTS FOR anyway_1 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 0 0 0 0 0 0 7 0 0 0 0 0 3 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR high_tm No mispriming library specified Using 0-based sequence positions WARNING: Left primer is unacceptable: Tm too high OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR high_tm_and_self_any No mispriming library specified Using 0-based sequence positions WARNING: Left primer is unacceptable: Tm too high/High self complementarity OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR high_tm_and_self_any_and_target No mispriming library specified Using 0-based sequence positions WARNING: Left primer is unacceptable: Overlaps Target/Tm too high/High self complementarity OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 20,122 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>)*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ********************** <<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Right 3 0 0 0 0 0 0 0 2 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR high_tm_and_self_any_and_excl_region No mispriming library specified Using 0-based sequence positions WARNING: Left primer is unacceptable: Overlaps Excluded Region/Tm too high/High self complementarity OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 EXCLUDED REGIONS (start, len)*: 20,1 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>X*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR high_tm_and_self_any_and_incl_region INPUT PROBLEM: Specified left primer not in Included Region PRIMER PICKING RESULTS FOR left_high_tm_and_self_any_and_right No mispriming library specified Using 0-based sequence positions WARNING: Left primer is unacceptable: Tm too high/High self complementarity OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR left_high_tm_and_self_any_and_right_bad_gc No mispriming library specified Using 0-based sequence positions WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Unacceptable GC content OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR left_high_tm_and_self_any_and_right_bad_gc_and_target No mispriming library specified Using 0-based sequence positions WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 143,1 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************(<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR internal_too_short No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: Specified internal oligo < PRIMER_INTERNAL_MIN_SIZE; Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content; Hybridization probe is unacceptable: Too short/Tm too low OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC INTERNAL OLIGO 23 13 41.12 53.85 6.00 2.00 gcgtacgatccat SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 143,1 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************(<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR internal_ok No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC INTERNAL OLIGO 23 19 59.42 57.89 6.00 6.00 gcgtacgatccatgctagc SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 143,1 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************(<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR internal_too_cold No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: Left primer is unacceptable: Tm too high/High self complementarity; Right primer is unacceptable: Overlaps Target/Unacceptable GC content; Hybridization probe is unacceptable: Tm too low OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC INTERNAL OLIGO 23 19 59.42 57.89 6.00 6.00 gcgtacgatccatgctagc SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 143,1 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************(<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR internal_too_cold2 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: Hybridization probe is unacceptable: Tm too low OLIGO start len tm gc% any 3' seq LEFT PRIMER 1 20 58.23 50.00 10.00 0.00 CCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC INTERNAL OLIGO 23 19 59.42 57.89 6.00 6.00 gcgtacgatccatgctagc SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 160, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR mispriming Using mispriming library not_for_production.lib No internal oligo mishyb library specified Using 0-based sequence positions WARNING: Left primer is unacceptable: High similarity to mispriming or mishyb library; Hybridization probe is unacceptable: Tm too low OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 1 20 58.23 50.00 10.00 0.00 9.00 CCTACAGCTGTGGGAAAATG RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 8.00 CAGTGTGTACATGGCGGC INTERNAL OLIGO 23 19 59.42 57.89 6.00 6.00 gcgtacgatccatgctagc SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 160, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 21,122 0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc >>>>>>>>>>>>>>>>>>>>*************************************** 60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac ************************************************************ 120 actaggccgctatatcatctagcGCCGCCATGTACACACTG ***********************<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Right 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 primer3-2.3.6/test/dpal_input0000664000077300007730000003256012231156245015125 0ustar ioanaioanaAAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA l AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA e AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA g AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA L AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG l AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG e AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG g AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG L AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC l AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC e AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC g AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC L ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA l ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA e ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA g ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA L TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG l TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG e TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG g TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG L AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG l AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG e AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG g AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG L AAACGATCGATTTT AAACGATCGACCCC l AAACGATCGATTTT AAACGATCGACCCC e AAACGATCGATTTT AAACGATCGACCCC g AAACGATCGATTTT AAACGATCGACCCC L AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT l AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT e AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT g AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT L AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC l AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC e AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC g AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC L ACACACACACACACA TGTGTTGGTGTTGTTGTGTG l ACACACACACACACA TGTGTTGGTGTTGTTGTGTG e ACACACACACACACA TGTGTTGGTGTTGTTGTGTG g ACACACACACACACA TGTGTTGGTGTTGTTGTGTG L TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG l TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG e TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG g TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG L TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT l TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT e TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT g TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT L TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG l TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG e TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG g TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG L AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG l AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG e AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG g AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG L AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT l AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT e AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT g AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT L AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG l AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG e AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG g AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG L TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG l TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG e TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG g TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG L -m 3 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA l -m 3 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA e -m 3 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA g -m 3 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA L -m 3 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG l -m 3 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG e -m 3 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG g -m 3 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG L -m 3 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC l -m 3 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC e -m 3 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC g -m 3 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC L -m 3 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA l -m 3 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA e -m 3 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA g -m 3 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA L -m 3 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG l -m 3 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG e -m 3 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG g -m 3 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG L -m 3 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG l -m 3 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG e -m 3 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG g -m 3 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG L -m 3 AAACGATCGATTTT AAACGATCGACCCC l -m 3 AAACGATCGATTTT AAACGATCGACCCC e -m 3 AAACGATCGATTTT AAACGATCGACCCC g -m 3 AAACGATCGATTTT AAACGATCGACCCC L -m 3 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT l -m 3 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT e -m 3 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT g -m 3 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT L -m 3 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC l -m 3 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC e -m 3 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC g -m 3 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC L -m 3 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG l -m 3 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG e -m 3 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG g -m 3 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG L -m 3 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG l -m 3 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG e -m 3 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG g -m 3 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG L -m 3 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT l -m 3 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT e -m 3 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT g -m 3 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT L -m 3 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG l -m 3 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG e -m 3 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG g -m 3 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG L -m 3 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG l -m 3 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG e -m 3 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG g -m 3 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG L -m 3 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT l -m 3 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT e -m 3 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT g -m 3 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT L -m 3 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG l -m 3 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG e -m 3 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG g -m 3 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG L -m 3 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG l -m 3 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG e -m 3 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG g -m 3 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG L -m 1 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA l -m 1 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA e -m 1 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA g -m 1 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA L -m 1 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG l -m 1 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG e -m 1 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG g -m 1 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG L -m 1 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC l -m 1 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC e -m 1 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC g -m 1 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC L -m 1 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA l -m 1 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA e -m 1 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA g -m 1 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA L -m 1 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG l -m 1 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG e -m 1 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG g -m 1 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG L -m 1 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG l -m 1 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG e -m 1 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG g -m 1 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG L -m 1 AAACGATCGATTTT AAACGATCGACCCC l -m 1 AAACGATCGATTTT AAACGATCGACCCC e -m 1 AAACGATCGATTTT AAACGATCGACCCC g -m 1 AAACGATCGATTTT AAACGATCGACCCC L -m 1 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT l -m 1 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT e -m 1 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT g -m 1 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT L -m 1 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC l -m 1 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC e -m 1 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC g -m 1 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC L -m 1 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG l -m 1 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG e -m 1 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG g -m 1 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG L -m 1 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG l -m 1 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG e -m 1 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG g -m 1 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG L -m 1 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT l -m 1 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT e -m 1 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT g -m 1 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT L -m 1 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG l -m 1 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG e -m 1 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG g -m 1 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG L -m 1 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG l -m 1 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG e -m 1 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG g -m 1 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG L -m 1 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT l -m 1 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT e -m 1 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT g -m 1 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT L -m 1 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG l -m 1 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG e -m 1 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG g -m 1 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG L -m 1 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG l -m 1 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG e -m 1 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG g -m 1 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG L -m 0 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA l -m 0 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA e -m 0 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA g -m 0 AAAACCCGCTTTGCTAGCTACG AACCCGCTTGCTAGCAAAAAAAA L -m 0 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG l -m 0 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG e -m 0 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG g -m 0 AAACACACACACACACT TGGGGGTGTTGGGGTTGTGG L -m 0 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC l -m 0 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC e -m 0 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC g -m 0 AAACGATCGTAGCGTTTCAGTCT AGTCTCTCTCTCTCTCTCTC L -m 0 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA l -m 0 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA e -m 0 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA g -m 0 ACGCCGTTGCGCGCTGCTGCG TTTTTTTTTTTTTTTTTTA L -m 0 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG l -m 0 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG e -m 0 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG g -m 0 TTTTTTTTTTATTTTTTTTT GGGGGGGGGAGGGGGGGGG L -m 0 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG l -m 0 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG e -m 0 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG g -m 0 AACCGGGTCTTTTTTTAACC AACCTTAAAAAAAAACCGG L -m 0 AAACGATCGATTTT AAACGATCGACCCC l -m 0 AAACGATCGATTTT AAACGATCGACCCC e -m 0 AAACGATCGATTTT AAACGATCGACCCC g -m 0 AAACGATCGATTTT AAACGATCGACCCC L -m 0 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT l -m 0 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT e -m 0 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT g -m 0 AAAAAAAAAAAAAAAT CCCCCCCCCCCCCT L -m 0 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC l -m 0 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC e -m 0 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC g -m 0 AAAAAAAAAAAAAAAT TCCCCCCCCCCCCC L -m 0 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG l -m 0 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG e -m 0 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG g -m 0 ACACACACACACACA TGTGTTGGTGTTGTTGTGTG L -m 0 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG l -m 0 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG e -m 0 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG g -m 0 TAAAAAAAAAAAAAA GGGGGGGGTGGGGGGGGG L -m 0 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT l -m 0 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT e -m 0 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT g -m 0 TAAAAAAAAAAAAAA GGGGGGGGGGGGGGGT L -m 0 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG l -m 0 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG e -m 0 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG g -m 0 TAAAAAAAAAAAAAA TGGGGGGGGGGGGGGG L -m 0 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG l -m 0 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG e -m 0 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG g -m 0 AAAAAAATAAAAAAA TGGGGGGGGGGGGGGG L -m 0 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT l -m 0 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT e -m 0 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT g -m 0 AAAAAAATAAAAAAA GGGGGGGGGGGGGGGT L -m 0 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG l -m 0 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG e -m 0 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG g -m 0 AAAAAAAAAAAAAAT GGGGGGGTGGGGGGGG L -m 0 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG l -m 0 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG e -m 0 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG g -m 0 TAAAAAAAAAAAAAA GGGGGGGTGGGGGGGG L "" "" l "" "" g "" "" e "" "" L A "" l A "" g A "" e A "" L "" A l "" A g "" A e "" A L AAGATCAGCTAGCTAGCTAGC TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT l AAGATCAGCTAGCTAGCTAGC TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT g AAGATCAGCTAGCTAGCTAGC TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT e AAGATCAGCTAGCTAGCTAGC TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT L GATCAGCTAGCTTTTT TTTTTTGATCAGCTAGCTTTTT l GATCAGCTAGCTTTTT TTTTTTGATCAGCTAGCTTTTT g GATCAGCTAGCTTTTT TTTTTTGATCAGCTAGCTTTTT e GATCAGCTAGCTTTTT TTTTTTGATCAGCTAGCTTTTT L AAGATCAGCTAGCTAGCTAGCTTTTT TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT l AAGATCAGCTAGCTAGCTAGCTTTTT TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT g AAGATCAGCTAGCTAGCTAGCTTTTT TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT e AAGATCAGCTAGCTAGCTAGCTTTTT TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT L CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L -g 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l -g 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g -g 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e -g 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L -g 4 -l 4 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l -g 4 -l 4 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g -g 4 -l 4 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e -g 4 -l 4 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L -g 3 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l -g 3 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g -g 3 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e -g 3 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L -g 6 -l 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l -g 6 -l 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g -g 6 -l 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e -g 6 -l 6 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L -g 5 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l -g 5 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g -g 5 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e -g 5 -l 3 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L -g 3 -l 5 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA l -g 3 -l 5 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA g -g 3 -l 5 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA e -g 3 -l 5 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L primer3-2.3.6/test/primer_mispriming_formatted_output0000664000077300007730000004570612231156245022215 0ustar ioanaioanaPRIMER PICKING RESULTS FOR exon1 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 43 18 55.22 55.56 6.00 1.00 9.00 TCACCCACGAGTGAAGAC RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC SEQUENCE SIZE: 365 INCLUDED REGION SIZE: 365 PRODUCT SIZE: 237, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 EXCLUDED REGIONS (start, len)*: 100,150 0 AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGA >>>>>>>>>>>>>>>>> 60 CTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCA > XXXXXXXXXXXXXXXXXXXX 120 GTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGC XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180 TGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAA XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240 GAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCA XXXXXXXXXX <<<<<<<<<<<<<<<<<< 300 GGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGC 360 GCCGG KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 40 18 55.24 50.00 8.00 8.00 8.00 GTTTCACCCACGAGTGAA RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC PRODUCT SIZE: 240, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 39 18 54.75 50.00 6.00 6.00 9.00 AGTTTCACCCACGAGTGA RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC PRODUCT SIZE: 241, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 3 LEFT PRIMER 38 18 54.75 55.56 4.00 4.00 8.00 GAGTTTCACCCACGAGTG RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC PRODUCT SIZE: 242, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00 4 LEFT PRIMER 43 18 55.22 55.56 6.00 1.00 9.00 TCACCCACGAGTGAAGAC RIGHT PRIMER 280 18 56.87 61.11 3.00 3.00 8.00 GCTCCTCAGCCTTGTCAC PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 877 1 0 91 0 7 0 2 399 0 0 0 0 0 377 Right 1012 19 0 58 0 25 0 0 702 0 0 0 0 0 208 Pair Stats: considered 23, unacceptable product size 17, ok 6 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR exon2 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 39 20 54.89 35.00 4.00 4.00 9.00 CCCAAATCCCATTATTCATA RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA SEQUENCE SIZE: 342 INCLUDED REGION SIZE: 342 PRODUCT SIZE: 229, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 EXCLUDED REGIONS (start, len)*: 100,145 0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA >>>>>>>>>>>>>>>>>>>> 60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA XXXXXXXXXXXXXXXXXXXX 120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA XXXXX<<<<<<<<<<<<<<<<<<<<<<< 300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 39 20 54.89 35.00 4.00 4.00 9.00 CCCAAATCCCATTATTCATA RIGHT PRIMER 268 24 54.78 29.17 6.00 6.00 9.00 ATGATGTCTACTTTCCAAATTACA PRODUCT SIZE: 230, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 2 LEFT PRIMER 21 20 54.19 45.00 6.00 4.00 8.00 CTTAAGTTTGTAACAGGGCC RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 3 LEFT PRIMER 20 20 54.19 45.00 6.00 2.00 8.00 CCTTAAGTTTGTAACAGGGC RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 4 LEFT PRIMER 19 21 55.19 42.86 6.00 2.00 8.00 ACCTTAAGTTTGTAACAGGGC RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA PRODUCT SIZE: 249, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 728 0 0 69 0 2 0 126 0 0 0 1 23 0 507 Right 827 0 0 72 0 314 0 236 0 0 0 0 0 0 205 Pair Stats: considered 9, unacceptable product size 4, ok 5 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR exon2 Using mispriming library not_for_production.lib No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 39 20 54.89 35.00 4.00 4.00 9.00 CCCAAATCCCATTATTCATA RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT SEQUENCE SIZE: 342 INCLUDED REGION SIZE: 342 PRODUCT SIZE: 229, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 EXCLUDED REGIONS (start, len)*: 100,145 0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA >>>>>>>>>>>>>>>>>>>> 60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA XXXXXXXXXXXXXXXXXXXX 120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA XXXXX<<<<<<<<<<<<<<<<<<<<<<< 300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 39 20 54.89 35.00 4.00 4.00 9.00 CCCAAATCCCATTATTCATA RIGHT PRIMER 268 24 54.78 29.17 6.00 6.00 9.00 ATGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT PRODUCT SIZE: 230, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 2 LEFT PRIMER 21 20 54.19 45.00 6.00 4.00 8.00 CTTAAGTTTGTAACAGGGCC RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 3 LEFT PRIMER 20 20 54.19 45.00 6.00 2.00 8.00 CCTTAAGTTTGTAACAGGGC RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 4 LEFT PRIMER 19 21 55.19 42.86 6.00 2.00 8.00 ACCTTAAGTTTGTAACAGGGC RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT PRODUCT SIZE: 249, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 728 0 0 69 0 2 0 126 0 0 0 1 23 0 507 Right 827 0 0 72 0 314 0 236 0 0 0 0 0 0 205 Intl 3079 0 0 0 0 316 0 517 178 0 0 0 23 0 2045 Pair Stats: considered 9, unacceptable product size 4, ok 5 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR exon1_2 Using mispriming library not_for_production.lib No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 40 18 55.24 50.00 8.00 8.00 8.00 GTTTCACCCACGAGTGAA RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC INTERNAL OLIGO 210 20 59.99 55.00 4.00 2.00 CTTCGAGTGGTCAAAGGAGC SEQUENCE SIZE: 365 INCLUDED REGION SIZE: 365 PRODUCT SIZE: 240, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 EXCLUDED REGIONS (start, len)*: 100,150 0 AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGA >>>>>>>>>>>>>>>>>> 60 CTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCA XXXXXXXXXXXXXXXXXXXX 120 GTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGC XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180 TGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAA XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240 GAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCA XXXXXXXXXX <<<<<<<<<<<<<<<<<< 300 GGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGC 360 GCCGG KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 38 18 54.75 55.56 4.00 4.00 8.00 GAGTTTCACCCACGAGTG RIGHT PRIMER 279 18 56.69 61.11 3.00 1.00 8.00 CTCCTCAGCCTTGTCACC INTERNAL OLIGO 210 20 59.99 55.00 4.00 2.00 CTTCGAGTGGTCAAAGGAGC PRODUCT SIZE: 242, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 43 18 55.22 55.56 6.00 1.00 9.00 TCACCCACGAGTGAAGAC RIGHT PRIMER 280 18 56.87 61.11 3.00 3.00 8.00 GCTCCTCAGCCTTGTCAC INTERNAL OLIGO 210 20 59.99 55.00 4.00 2.00 CTTCGAGTGGTCAAAGGAGC PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 3 LEFT PRIMER 40 18 55.24 50.00 8.00 8.00 8.00 GTTTCACCCACGAGTGAA RIGHT PRIMER 280 18 56.87 61.11 3.00 3.00 8.00 GCTCCTCAGCCTTGTCAC INTERNAL OLIGO 210 20 59.99 55.00 4.00 2.00 CTTCGAGTGGTCAAAGGAGC PRODUCT SIZE: 241, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 4 LEFT PRIMER 39 18 54.75 50.00 6.00 6.00 9.00 AGTTTCACCCACGAGTGA RIGHT PRIMER 280 18 56.87 61.11 3.00 3.00 8.00 GCTCCTCAGCCTTGTCAC INTERNAL OLIGO 210 20 59.99 55.00 4.00 2.00 CTTCGAGTGGTCAAAGGAGC PRODUCT SIZE: 242, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 4.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 877 1 0 91 0 7 0 2 399 0 0 0 0 0 377 Right 1012 19 0 58 0 25 0 0 702 0 0 0 0 0 208 Intl 3237 27 0 0 0 32 0 23 1880 0 0 0 0 0 1275 Pair Stats: considered 26, unacceptable product size 17, high mispriming library similarity 2, ok 7 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR exon2_2 Using mispriming library not_for_production.lib No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 23 19 55.24 47.37 6.00 6.00 8.00 TAAGTTTGTAACAGGGCCC RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT SEQUENCE SIZE: 342 INCLUDED REGION SIZE: 342 PRODUCT SIZE: 245, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 EXCLUDED REGIONS (start, len)*: 100,145 0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA >>>>>>>>>>>>>>>>>>> 60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA XXXXXXXXXXXXXXXXXXXX 120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA XXXXX<<<<<<<<<<<<<<<<<<<<<<< 300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 23 19 55.24 47.37 6.00 6.00 8.00 TAAGTTTGTAACAGGGCCC RIGHT PRIMER 268 24 54.78 29.17 6.00 6.00 9.00 ATGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT PRODUCT SIZE: 246, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 2 LEFT PRIMER 22 20 56.79 45.00 6.00 6.00 8.00 TTAAGTTTGTAACAGGGCCC RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT PRODUCT SIZE: 246, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00 3 LEFT PRIMER 24 18 55.03 50.00 6.00 6.00 8.00 AAGTTTGTAACAGGGCCC RIGHT PRIMER 267 23 54.24 30.43 6.00 6.00 9.00 TGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT PRODUCT SIZE: 244, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 4 LEFT PRIMER 22 20 56.79 45.00 6.00 6.00 8.00 TTAAGTTTGTAACAGGGCCC RIGHT PRIMER 268 24 54.78 29.17 6.00 6.00 9.00 ATGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00 5 LEFT PRIMER 24 18 55.03 50.00 6.00 6.00 8.00 AAGTTTGTAACAGGGCCC RIGHT PRIMER 268 24 54.78 29.17 6.00 6.00 9.00 ATGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT PRODUCT SIZE: 245, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 6 LEFT PRIMER 23 19 55.24 47.37 6.00 6.00 8.00 TAAGTTTGTAACAGGGCCC RIGHT PRIMER 269 25 54.96 28.00 6.00 6.00 9.00 TATGATGTCTACTTTCCAAATTACA INTERNAL OLIGO 127 20 59.94 40.00 6.00 2.00 GTTTGGCATGATTTTGCCTT PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 728 0 0 69 0 2 0 126 0 0 0 14 23 0 494 Right 827 0 0 72 0 314 0 236 0 0 0 19 0 0 186 Intl 3079 0 0 0 0 316 0 517 178 0 0 0 23 0 2045 Pair Stats: considered 167, unacceptable product size 101, tm diff too large 12, high mispriming library similarity 46, ok 8 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR exon2_3 Using mispriming library not_for_production.lib No internal oligo mishyb library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 342 INCLUDED REGION SIZE: 342 EXCLUDED REGIONS (start, len)*: 100,145 0 CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAA 60 TGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAA XXXXXXXXXXXXXXXXXXXX 120 AAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTG XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180 ATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAG XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240 TAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAA XXXXX 300 TGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 728 0 0 69 0 2 0 126 0 0 0 124 23 0 384 Right 827 0 0 72 0 314 0 236 0 0 0 176 0 0 29 Intl 3079 0 0 0 0 316 0 517 178 0 0 0 23 0 2045 Pair Stats: considered 12459, unacceptable product size 6872, tm diff too large 4075, high mispriming library similarity 1512, ok 0 primer3 release 2.0.0 primer3-2.3.6/test/primer_start_codon_input0000664000077300007730000010603612231156245020102 0ustar ioanaioanaPRIMER_PRODUCT_SIZE_RANGE=75-20000 PRIMER_MAX_END_STABILITY=10 PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_SIZE=19 PRIMER_MAX_TM=75 PRIMER_NUM_RETURN=1 SEQUENCE_ID=test0 SEQUENCE_TEMPLATE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA SEQUENCE_START_CODON_POSITION=-3 = SEQUENCE_ID=test1 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA SEQUENCE_START_CODON_POSITION=0 = SEQUENCE_ID=test2 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA SEQUENCE_START_CODON_POSITION=0 = SEQUENCE_ID=test3 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA SEQUENCE_START_CODON_POSITION=0 = SEQUENCE_ID=test4 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA SEQUENCE_START_CODON_POSITION=0 = SEQUENCE_ID=test5 SEQUENCE_TEMPLATE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA SEQUENCE_START_CODON_POSITION=0 = SEQUENCE_ID=test6 SEQUENCE_INCLUDED_REGION=0,299 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAATGATACTTCATTCCAGA SEQUENCE_START_CODON_POSITION=298 P3_COMMENT=Should fail with "Start codon not contained in INCLUDED_REGION". = SEQUENCE_ID=test7 SEQUENCE_INCLUDED_REGION=0,300 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATAATGATGATACTTCATTCCATG SEQUENCE_START_CODON_POSITION=297 P3_COMMENT=Should succeed. = SEQUENCE_ID=test8 PRIMER_INSIDE_PENALTY=100.0 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA SEQUENCE_START_CODON_POSITION=0 = SEQUENCE_ID=test9 PRIMER_INSIDE_PENALTY=-1.0 PRIMER_OUTSIDE_PENALTY=100.0 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA SEQUENCE_START_CODON_POSITION=0 = PRIMER_PRODUCT_SIZE_RANGE=75-20000 PRIMER_OUTSIDE_PENALTY=0.0 PRIMER_MAX_END_STABILITY=10 PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_SIZE=19 PRIMER_MAX_TM=65 PRIMER_NUM_RETURN=20 SEQUENCE_ID=DCC/X76132 SEQUENCE_TEMPLATE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGCGCGCATCTTCAAGTAACCGGTTTTCAAATTAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAAACATACTTCATTCCAGACACCACAAGCCAGATGAGGGACTTTACCAATGTGAGGCATCTTTAGGAGATTCTGGCTCAATTATTAGTCGGACAGCAAAAGTTGCAGTAGCAGGACCACTGAGGTTCCTTTCACAGACAGAATCTGTCACAGCCTTCATGGGAGACACAGTGCTACTCAAGTGTGAAGTCATTGGGGAGCCCATGCCAACAATCCACTGGCAGAAGAACCAACAAGACCTGACTCCAATCCCAGGTGACTCCCGAGTGGTGGTCTTGCCCTCTGGAGCATTGCAGATCAGCCGACTCCAACCGGGGGACATTGGAATTTACCGATGCTCAGCTCGAAATCCAGCCAGCTCAAGAACAGGAAATGAAGCAGAAGTCAGAATTTTATCAGATCCAGGACTGCATAGACAGCTGTATTTTCTGCAAAGACCATCCAATGTAGTAGCCATTGAAGGAAAAGATGCTGTCCTGGAATGTTGTGTTTCTGGCTATCCTCCACCAAGTTTTACCTGGTTACGAGGCGAGGAAGTCATCCAACTCAGGTCTAAAAAGTATTCTTTATTGGGTGGAAGCAACTTGCTTATCTCCAATGTGACAGATGATGACAGTGGAATGTATACCTGTGTTGTCACATATAAAAATGAGAATATTAGTGCCTCTGCAGAGCTCACAGTCTTGGTTCCGCCATGGTTTTTAAATCATCCTTCCAACCTGTATGCCTATGAAAGCATGGATATTGAGTTTGAATGTACAGTCTCTGGAAAGCCTGTGCCCACTGTGAATTGGATGAAGAATGGAGATGTGGTCATTCCTAGTGATTATTTTCAGATAGTGGGAGGAAGCAACTTACGGATACTTGGGGTGGTGAAGTCAGATGAAGGCTTTTATCAATGTGTGGCTGAAAATGAGGCTGGAAATGCCCAGACCAGTGCACAGCTCATTGTCCCTAAGCCTGCAATCCCAAGCTCCAGTGTCCTCCCTTCGGCTCCCAGAGATGTGGTCCCTGTCTTGGTTTCCAGCCGATTTGTCCGTCTCAGCTGGCGCCCACCTGCAGAAGCGAAAGGGAACATTCAAACTTTCACGGTCTTTTTCTCCAGAGAAGGTGACAACAGGGAACGAGCATTGAATACAACACAGCCTGGGTCCCTTCAGCTCACTGTGGGAAACCTGAAGCCAGAAGCCATGTACACCTTTCGAGTTGTGGCTTACAATGAATGGGGACCGGGAGAGAGTTCTCAACCCATCAAGGTGGCCACACAGCCTGAGTTGCAAGTTCCAGGGCCAGTAGAAAACCTGCAAGCTGTATCTACCTCACCTACCTCAATTCTTATTACCTGGGAACCCCCTGCCTATGCAAACGGTCCAGTCCAAGGTTACAGATTGTTCTGCACTGAGGTGTCCACAGGAAAAGAACAGAATATAGAGGTTGATGGACTATCTTATAAACTGGAAGGCCTGAAAAAATTCACCGAATATAGTCTTCGATTCTTAGCTTATAATCGCTATGGTCCGGGCGTCTCTACTGATGATATAACAGTGGTTACACTTTCTGACGTGCCAAGTGCCCCGCCTCAGAACGTCTCCCTGGAAGTGGTCAATTCAAGAAGTATCAAAGTTAGCTGGCTGCCTCCTCCATCAGGAACACAAAATGGATTTATTACCGGCTATAAAATTCGACACAGAAAGACGACCCGCAGGGGTGAGATGGAAACACTGGAGCCAAACAACCTCTGGTACCTATTCACAGGACTGGAGAAAGGAAGTCAGTACAGTTTCCAGGTGTCAGCCATGACAGTCAATGGTACTGGACCACCTTCCAACTGGTATACTGCAGAGACTCCAGAGAATGATCTAGATGAATCTCAAGTTCCTGATCAACCAAGCTCTCTTCATGTGAGGCCCCAGACTAACTGCATCATCATGAGTTGGACTCCTCCCTTGAACCCAAACATCGTGGTGCGAGGTTATATTATCGGTTATGGCGTTGGGAGCCCTTACGCTGAGACAGTGCGTGTGGACAGCAAGCAGCGATATTATTCCATTGAGAGGTTAGAGTCAAGTTCCCATTATGTAATCTCCCTAAAAGCTTTTAACAATGCCGGAGAAGGAGTTCCTCTTTATGAAAGTGCCACCACCAGGTCTATAACCGATCCCACTGACCCAGTTGATTATTATCCTTTGCTTGATGATTTCCCCACCTCGGTCCCAGATCTCTCCACCCCCATGCTCCCACCAGTAGGTGTACAGGCTGTGGCTCTTACCCATGATGCTGTGAGGGTCAGCTGGGCAGACAACTCTGTCCCTAAGAACCAAAAGACGTCTGAGGTGCGACTTTACACCGTCCGGTGGAGAACCAGCTTTTCTGCAAGTGCAAAATACAAGTCAGAAGACACAACATCTCTAAGTTACACAGCAACAGGCCTCAAACCAAACACAATGTATGAATTCTCGGTCATGGTAACAAAAAACAGAAGGTCCAGTACTTGGAGCATGACTGCACATGCCACCACGTATGAAGCAGCCCCCACCTCTGCTCCCAAGGACTTTACAGTCATTACTAGGGAAGGGAAGCCTCGTGCCGTCATTGTGAGTTGGCAGCCTCCCTTGGAAGCCAATGGGAAAATTACTGCTTACATCTTATTTTATACCTTGGACAAGAACATCCCAATTGATGACTGGATTATGGAAACAATCAGTGGTGATAGGCTTACTCATCAAATCATGGATCTCAACCTTGATACTATGTATTACTTTCGAATTCAAGCACGAAATTCAAAAGGAGTGGGGCCACTCTCTGATCCCATCCTCTTCAGGACTCTGAAAGTGGAACACCCTGACAAAATGGCTAATGACCAAGGTCGTCATGGAGATGGAGGTTATTGGCCAGTTGATACTAATTTGATTGATAGAAGCACCCTAAATGAGCCGCCAATTGGACAAATGCACCCCCCGCATGGCAGTGTCACTCCTCAGAAGAACAGCAACCTGCTTGTGATCATTGTGGTCACCGTTGGTGTCATCACAGTGCTGGTAGTGGTCATCGTGGCTGTGATTTGCACCCGACGCTCTTCAGCCCAGCAGAGAAAGAAACGGGCCACCCACAGTGCTGGCAAAAGGAAGGGCAGCCAGAAGGACCTCCGACCCCCTGATCTTTGGATCCATCATGAAGAAATGGAGATGAAAAATATTGAAAAGCCATCTGGCACTGACCCTGCAGGAAGGGACTCTCCCATCCAAAGTTGCCAAGACCTCACACCAGTCAGCCACAGCCAGTCAGAAACCCAACTGGGAAGCAAAAGCACCTCTCATTCAGGTCAAGACACTGAGGAAGCAGGGAGCTCTATGTCCACTCTGGAGAGGTCGCTGGCTGCACGCCGAGCCCCCCGGGCCAAGCTCATGATTCCCATGGATGCCCAGTCCAACAATCCTGCTGTCGTGAGCGCCATCCCGGTGCCAACGCTAGAAAGTGCCCAGTACCCAGGAATCCTCCCGTCTCCCACCTGTGGATATCCCCACCCGCAGTTCACTCTCCGGCCTGTGCCATTCCCAACACTCTCAGTGGACCGAGGTTTCGGAGCAGGAAGAAGTCAGTCAGTGAGTGAAGGACCAACTACCCAACAACCACCTATGCTGCCCCCATCTCAGCCTGAGCATTCTAGCAGCGAGGAGGCACCAAGCAGAACCATCCCCACAGCTTGTGTTCGACCAACTCACCCACTCCGCAGCTTTGCTAATCCTTTGCTACCTCCACCAATGAGTGCAATAGAACCGAAAGTCCCTTACACACCACTTTTGTCTCAGCCAGGGCCCACTCTTCCTAAGACCCATGTGAAAACAGCCTCCCTTGGGTTGGCTGGAAAAGCAAGATCCCCTTTGCTTCCTGTGTCTGTGCCAACAGCCCCTGAAGTGTCTGAGGAGAGCCACAAACCAACAGAGGATTCAGCCAATGTGTATGAACAGGATGATCTGAGTGAACAAATGGCAAGTTTGGAAGGACTCATGAAGCAGCTTAATGCCATCACAGGCTCAGCCTTTTAACATGTATTTCTGAATGGATGAGGTGAATTTTCCGGGAACTTTGCAGCATACCAATTACCCATAAACAGCACACCTGTGTCCAAGAACTCTAACCAGTGTACAGGTCACCCATCAGGACCACTCAGTTAAGGAAGATCCTGAAGCAGTTCAGAAGGAATAAGCATTCCTTCTTTCACAGGCATCAGGAATTGTCAAATGATGATTATGAGTTCCCTAAACAAAAGCAAAGATGCATTTTCACTGCAATGTCAAAGTTTAGCTGCT SEQUENCE_START_CODON_POSITION=0 = SEQUENCE_ID=LEU8/L-SELECTING/X17519 SEQUENCE_TEMPLATE=CTCCCTTTGGGCAAGGACCTGAGACCCTTGTGCTAAGTCAAGAGGCTCAATGGGCTGCAGAAGAACTAGAGAAGGACCAAGCAAAGCCATGATATTTCCATGGAAATGTCAGAGCACCCAGAGGGACTTATGGAACATCTTCAAGTTGTGGGGGTGGACAATGCTCTGTTGTGATTTCCTGGCACATCATGGAACCGACTGCTGGACTTACCATTATTCTGAAAAACCCATGAACTGGCAAAGGGCTAGAAGATTCTGCCGAGACAATTACACAGATTTAGTTGCCATACAAAACAAGGCGGAAATTGAGTATCTGGAGAAGACTCTGCCTTTCAGTCGTTCTTACTACTGGATAGGAATCCGGAAGATAGGAGGAATATGGACGTGGGTGGGAACCAACAAATCTCTCACTGAAGAAGCAGAGAACTGGGGAGATGGTGAGCCCAACAACAAGAAGAACAAGGAGGACTGCGTGGAGATCTATATCAAGAGAAACAAAGATGCAGGCAAATGGAACGATGACGCCTGCCACAAACTAAAGGCAGCCCTCTGTTACACAGCTTCTTGCCAGCCCTGGTCATGCAGTGGCCATGGAGAATGTGTAGAAATCATCAATAATTACACCTGCAACTGTGATGTGGGGTACTATGGGCCCCAGTGTCAGTTTGTGATTCAGTGTGAGCCTTTGGAGGCCCCAGAGCTGGGTACCATGGACTGTACTCACTCTTTGGGAAACTTCAGCTTCAGCTCACAGTGTGCCTTCAGCTGCTCTGAAGGAACAAACTTAACTGGGATTGAAGAAACCACCTGTGGACCATTTGGAAACTGGTCATCTCCAGAACCAACCTGTCAAGTGATTCAGTGTGAGCCTCTATCAGCACCAGATTTGGGGATCATGAACTGTAGCCATCCCCTGGCCAGCTTCAGCTTTACCTCTGCATGTACCTTCATCTGCTCAGAAGGAACTGAGTTAATTGGGAAGAAGAAAACCATTTGTGAATCATCTGGAATCTGGTCAAATCCTAGTCCAATATGTCAAAAATTGGACAAAAGTTTCTCAATGATTAAGGAGGGTGATTATAACCCCCTCTTCATTCCAGTGGCAGTCATGGTTACTGCATTCTCTGGGTTGGCATTTATCATTTGGCTGGCAAGGAGATTAAAAAAAGGCAAGAAATCCAAGAGAAGTATGAATGACCCATATTAAATCGCCCTTGGTGAAAGAAAATTCTTGGAATACTAAAAATCATGAGATCCTTTAAATCCTTCCATGAAACGTTTTGTGTGGTGGCACCTCCTACGTCAAACATGAAGTGTGTTTCCTTCAGTGCATCTGGGAAGATTTCTACCTGACCAACAGTTCCTTCAGCTTCCATTTCGCCCCTCATTTATCCCTCAACCCCCAGCCCACAGGTGTTTATACAGCTCAGCTTTTTGTCTTTTCTGAGGAGAAACAAATAAGACCATAAAGGGAAAGGATTCATGTGGAATATAAAGATGGCTGACTTTGCTCTTTCTTGACTCTTGTTTTCAGTTTCAATTCAGTGCTGTACTTGATGACAGACACTTCTAAATGAAGTGCAAATTTGATACATATGTGAATATGGACTCAGTTTTCTTGCAGATCAAATTTCACGTCGTCTTCTGTATACTGTGGAGGTACACTCTTATAGAAAGTTCAAAAAGTCTACGCTCTCCTTTCTTTCTAACTCCAGTGAAGTAATGGGGTCCTGCTCAAGTTGAAAGAGTCCTATTTGCACTGTAGCCTCGCCGTCTGTGAATTGGACCATCCTATTTAACTGGCTTCAGCCTCCCCACCTTCTTCAGCCACCTCTCTTTTTCAGTTGGCTGACTTCCACACCTAGCATCTCATGAGTGCCAAGCAAAAGGAGAGAAGAGAGAAATAGCCTGCGCTGTTTTTTAGTTTGGGGGTTTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTTTCTTTTATCACGATATTATTAGTAAGAAAACATCACTGAAATGCTAGCTGCAAGTGACATCTCTTTGATGTCATATGGAAGAGTTAAAACAGGTGGAGAAATTCCTTGATTCACAATGAAATGCTCTCCTTTCCCCTGCCCCCAGACCTTTTATCCGACTTACCTAGATTCTACATATTCTTTAAATTTCATCTCAGGCCTCCCTCAACCCCACCACTTCTTTTATAACTAGTCCTTTACTAATCCAACCCATGATGAGCTCCTCTTCCTGGCTTCTTACTGAAAGGTTACCCTGTAACATGCAATTTTGCATTTGAATAAAGCCTGCTTTTTAAGTGTT SEQUENCE_START_CODON_POSITION=49 = SEQUENCE_ID=L40146/UBC4 SEQUENCE_START_CODON_POSITION=0 SEQUENCE_TEMPLATE=ATGGCGCTGAAAAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACCAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAATGGACTCAGAAGTATGCGATGTAA = SEQUENCE_ID=U51903/IQGAP2 SEQUENCE_START_CODON_POSITION=222 SEQUENCE_PRIMER=ATGCCACACGAAGAGCTGC SEQUENCE_TEMPLATE=GAGGGAGGAGAGTTCACTTTTACTTCAGTGTCAGCGCGCGGCGGCCGTGGCTGGCTCTGGCGAGAGAGCACCGAGGGAGTGGGTCGCAGATCTTCGGGCGGCTAGGGGAAATCGGCGAGAGGCGGGATCCGAGCGCGCCGGCGGGGCGCAGAGCCCGCGAGCCTGGCCAGCGAGGGTAGCCGCGGGGGGCGCGCCCCGGGCGGGCCCCCGGAGACGCGCAGGATGCCACACGAAGAGCTGCCGTCGCTGCAGAGACCCCGCTATGGCTCTATTGTGGACGATGAAAGGCTCTCTGCAGAGGAGATGGATGAGAGGAGGCGGCAGAACATTGCTTATGAATATCTGTGCCACTTAGAGGAAGCCAAAAGGTGGATGGAAGTTTGCTTAGTTGAAGAATTGCCACCAACCACTGAATTGGAAGAAGGGCTCCGGAATGGAGTTTACCTTGCAAAGTTAGCCAAGTTCTTTGCCCCGAAAATGGTATCAGAGAAAAAGATCTATGATGTGGAACAAACACGTTATAAGAAGTCTGGCCTTCATTTTCGACACACAGATAATACCGTCCAGTGGTTAAGAGCGATGGAGTCTATTGGTCTACCCAAGATATTTTATCCAGAAACAACAGATGTCTATGATCGGAAAAACATACCAAGAATGATATATTGCATTCACGCACTGAGTTTGTATCTGTTCAAACTAGGAATAGCACCCCAGATCCAGGATTTGTTGGGCAAAGTAGACTTCACAGAGGAGGAAATCAGTAATATGAGAAAAGAACTTGAGAAATATGGAATACAGATGCCATCTTTCAGCAAAATAGGTGGTATTCTGGCCAATGAACTGTCCGTGGATGAAGCTGCATTACATGCTGCAGTTATAGCCATTAATGAAGCAGTTGAAAAAGGAATAGCAGAGCAAACCGTTGTAACACTAAGAAACCCAAATGCGGTTTTAACTTTAGTGGATGACAACCTTGCACCAGAATATCAGAAAGAACTCTGGGATGCCAAAAAGAAAAAAGAGGAAAATGCAAGACTGAAGAATAGCTGTATTTCAGAAGAAGAAAGAGATGCTTATGAAGAACTGCTGACACAAGCAGAAATCCAAGGCAATATTAATAAAGTCAACAGGCAGGCTGCAGTGGACCATATCAATGCTGTCATTCCGGAAGGTGACCCCGAGAATACGCTGCTTGCACTGAAGAAACCAGAGGCCCAGCTGCCTGCTGTTTATCCCTTTGCTGCTGCCATGTATCAGAACGAACTTTTCAACCTCCAGAAACAGAACACCATGAACTACTTGGCCCACGAGGAGCTTTTGATTGCTGTGGAAATGTTGTCTGCTGTTGCTTTACTAAACCAGGCCTTGGAAAGCAACGATCTTGTGTCTGTGCAGAATCAACTCAGAAGCCCCGCAATAGGCTTAAACAATCTGGACAAGGCATATGTGGAACGTTATGCAAACACACTACTCTCTGTTAAACTAGAAGTTTTATCCCAAGGGCAAGATAACTTAAGCTGGAATGAAATTCAGAATTGTATTGATATGGTTAATGCTCAAATTCAAGAAGAAAATGACCGAGTTGTAGCTGTAGGGTACATCAATGAAGCTATTGATGAAGGGAATCCTTTGAGGACTTTAGAAACTTTGCTCCTACCTACTGCGAATATTAGTGATGTGGACCCAGCCCATGCCCAGCACTACCAGGATGTTTTATACCATGCTAAATCACAGAAACTCGGAGACTCTGAGAGTGTTTCCAAAGTGCTTTGGCTGGATGAGATACAGCAAGCCGTCGATGAGGCCAACGTGGACGAGGACAGAGCAAAACAATGGGTTACTCTGGTGGTTGATGTTAATCAGTGTTTGGAAGGAAAAAAATCAAGTGATATTTTGTCTGTATTGAAGTCTTCCACTTCTAATGCAAATGACATAATCCCGGAGTGTGCTGACAAATACTATGATGCCCTTGTGAAGGCAAAAGAGCTCAAATCTGAAAGAGTGTCTAGTGACGGTTCATGGCTCAAACTCAACCTGCACAAAAAATATGACTACTATTACAACACTGATTCAAAAGAGAGTTCCTGGGTCACACCTGAATCATGCTTCTATAAAGAATCATGGCTCACAGGAAAAGAAATCGAGGACATTATTGAGGAAGTCACAGTAGGTTACATTCGTGAGAATATATGGTCTGCTTCAGAAGAGTTGCTTCTTCGCTTTCAAGCCACAAGCTCAGGACCCATCCTTAGGGAAGAGTTTGAAGCTAGAAAATCATTTTTGCATGAACAAGAAGAGAATGTGGTCAAAATACAGGCTTTTTGGAAAGGATATAAACAACGGAAGGAGTATATGCACAGGCGGCAAACGTTCATTGATAATACTGATTCTGTTGTGAAGATTCAGTCCTGGTTCCGAATGGCAACTGCAAGAAAGAGCTATCTTTCAAGACTACAGTATTTCAGAGATCATAATAATGAAATTGTGAAAATACAGTCACTGTTGAGAGCGAACAAAGCTAGAGATGACTACAAAACATTGGTTGGCTCTGAAAACCCACCATTAACAGTAATTCGCAAATTTGTATACCTGCTGGACCAAAGTGATTTGGATTTCCAGGAGGAACTAGAGGTTGCACGATTAAGGGAAGAAGTAGTGACCAAGATCAGGGCCAATCAACAGCTGGAAAAAGACCTGAACCTGATGGACATCAAGATTGGACTGCTGGTGAAGAACAGGATCACACTAGAGGATGTAATTTCACACAGTAAAAAGCTGAACAAGAAAAAAGGAGGAGAAATGGAAATACTGAATAACACCGACAACCAAGGAATAAAAAGTTTGAGTAAGGAGAGGAGAAAAACACTAGAAACATATCAGCAGCTGTTTTACCTTTTACAGACCAACCCTTTATACTTGGCTAAGCTGATTTTCCAGATGCCACAGAACAAGTCCACTAAATTTATGGATACTGTTATTTTCACACTATATAATTATGCCTCTAATCAGCGAGAAGAATATCTACTTCTCAAGCTTTTTAAAACTGCTCTGGAGGAAGAAATAAAATCAAAAGTGGACCAGGTACAGGACATAGTTACTGGTAACCCTACAGTCATCAAGATGGTCGTCAGCTTCAATAGAGGTGCCCGGGGACAGAACACCCTGCGCCAACTCCTGGCTCCAGTGGTAAAAGAGATCATCGACGACAAGTCGCTGATTATCAACACAAACCCTGTAGAGGTGTACAAGGCTTGGGTGAACCAACTAGAAACACAGACTGGAGAGGCCAGCAAGTTGCCTTATGATGTGACCACAGAACAAGCTCTAACATACCCAGAAGTGAAAAATAAACTGGAGGCTTCCATTGAGAACCTGAGAAGGGTCACCGACAAAGTCCTGAATTCTATCATTTCTTCCCTTGATCTACTGCCTTATGGATTGAGGTATATAGCCAAAGTACTGAAGAATTCGATCCATGAGAAATTCCCCGATGCAACAGAAGATGAGCTATTAAAGATTGTTGGAAACCTCCTGTACTATCGGTACATGAATCCAGCCATTGTAGCTCCAGATGGCTTTGATATCATCGACATGACAGCTGGAGGTCAGATAAATTCTGACCAAAGGAGAAACTTAGGATCAGTGGCCAAGGTTCTTCAGCACGCAGCCTCCAACAAGCTGTTTGAAGGAGAAAATGAGCATCTCTCATCTATGAACAATTATTTATCAGAGACGTATCAGGAATTCAGGAAATATTTCAAAGAAGCATGTAATGTCCCTGAGCCAGAAGAGAAGTTTAATATGGACAAATACACAGACCTGGTGACAGTCAGCAAACCAGTCATTTATATTTCAATTGAAGAAATCATCAGCACACACTCACTCCTGTTGGAACACCAGGATGCAATTGCCCCTGAGAAAAATGACTTACTGAGTGAATTGCTGGGGTCGCTGGGAGAGGTGCCAACCGTGGAATCTTTTCTTGGGGAAGGAGCAGTTGACCCCAATGACCCTAACAAGGCAAATACACTAAGTCAGCTTTCAAAGACCGAGATTTCTCTTGTCTTGACAAGCAAATATGACATAGAGGACGGTGAAGCTATAGATAGCCGAAGCCTCATGATAAAGACCAAGAAGCTGATAATTGATGTGATCCGGAACCAGCCAGGGAACACATTGACAGAAATCTTAGAGACACCAGCAACTGCGCAACAGGAGGTAGACCATGCCACGGACATGGTGAGCCGTGCAATGATAGATTCCAGGACTCCAGAAGAAATGAAGCATAGCCAATCTATGATTGAAGATGCACAGCTGCCTCTTGAGCAGAAGAAGAGGAAAATCCAGAGGAATCTTCGGACGTTGGAACAGACTGGACACGTGTCATCCGAAAATAAATACCAAGACATTCTCAATGAGATTGCCAAGGATATTCGAAATCAAAGAATCTATCGTAAGCTTCGAAAAGCTGAATTGGCAAAACTTCAGCAGACCCTGAATGCACTTAACAAGAAGGCAGCATTTTATGAAGAGCAAATCAATTATTATGACACCTACATAAAGACTTGTTTAGACAACTTAAAAAGAAAAAATACTCGGAGATCAATTAAACTAGATGGAAAAGGAGAACCCAAAGGGGCGAAGAGAGCGAAGCCAGTGAAGTACACTGCAGCAAAGCTGCATGAGAAAGGTGTCCTGCTAGATATAGATGATCTTCAAACAAACCAGTTTAAGAATGTTACATTTGATATCATAGCTACTGAAGATGTAGGCATTTTCGATGTAAGATCAAAATTCCTTGGTGTTGAGATGGAAAAGGTGCAACTCAATATTCAGGATTTACTTCAGATGCAATATGAAGGAGTAGCTGTAATGAAAATGTTTGATAAGGTTAAAGTGAATGTAAACCTTCTCATATACCTGCTGAACAAGAAGTTCTATGGAAAGTGAAGTGCCTACAGAAATTTCTTGGATTCTGTATCATCTGGATTAGGAAATGAATTTGTTTAATATTTTTGTTTTTAAACATGATTGAAATCACTGCTTATAAATGTGTGATTTTTTTTAAATGACCAAAACTGTTCTGAAGAATGTACCCAGGTGCCTTTTTGCTAATTTGATACTATAATAGAATGAGACATAAAATGAATTAATGGAAACATATCCACACTGTACTGTGATATAGGTACTCTGATTTAAAACTTTGGACATCCTGTGATCTGTTTTAAAGTTGGGGGGTGGGAAATTTAGCTGACTAGGGACAAACATGTAAACCTATTTTCCTATGAAAAAAGTTTTAAATGTCCCACTTGAATAACGTAATTCTTCATAGTTTTTTTAATCTATGGATAAATGGAAACCTAATTATTTGTAATGAATTATTTAGACAGTTCTAAGCCCTGTCTTCTGGGAGTTATCAATTTTAAAGAGAACTTTTGTGCAATTCAAATGAAGTTTTTATAAGTAATTGAAAATGACAACACAATAACACTTTCTGTATAAAAGTATATATTTTATGTGATTTATTCCTACTAAATGAAAGTGCACTACTGCCTCATGTAAAGACTCTTGCACGCAGAGCCTTTAAGTGACTAAGGAACAACATAGATAGTGAGCATAGTCCCCACCTCCACCCCTCACAATTTATTTGAATACTTCAATTGTGCCTCTCAATTTTTTGTAATGCTAAAAAATCAGTATCTAGATGGTTTTTAAATGTATTCTCTGGAAATTGTTTTATGTAAAATAAATGTTACTTAATTCCATT = SEQUENCE_ID=M59040/CD44 P3_COMMENT=Human cell adhesion molecule (CD44) mRNA, complete cds. CDS_29..1114 SEQUENCE_START_CODON_POSITION=28 SEQUENCE_TEMPLATE=CCCGCGCCCTCCGTTCGCTCCGGACACCATGGACAAGTTTTGGTGGCACGCAGCCTGGGGACTCTGCCTCGTGCCGCTGAGCCTGGCGCAGATCGATTTGAATATAACCTGCCGCTTTGCAGGTGTATTCCACGTGGAGAAAAATGGTCGCTACAGCATCTCTCGGACGGAGGCCGCTGACCTCTGCAAGGCTTTCAATAGCACCTTGCCCACAATGGCCCAGATGGAGAAAGCTCTGAGCATCGGATTTGAGACCTGCAGGTATGGGTTCATAGAAGGGCATGTGGTGATTCCCCGGATCCACCCCAACTCCATCTGTGCAGCAAACAACACAGGGGTGTACATCCTCACATACAACACCTCCCAGTATGACACATATTGCTTCAATGCTTCAGCTCCACCTGAAGAAGATTGTACATCAGTCACAGACCTGCCCAATGCCTTTGATGGACCAATTACCATAACTATTGTTAACCGTGATGGCACCCGCTATGTCCAGAAAGGAGAATACAGAACGAATCCTGAAGACATCTACCCCAGCAACCCTACTGATGATGACGTGAGCAGCGGCTCCTCCAGTGAAAGGAGCAGCACTTCAGGAGGTTACATCTTTTACACCTTTTCTACTGTACACCCCATCCCAGACGAAGACAGTCCCTGGATCACCGACAGCACAGACAGAATCCCTGCTACCAGAGACCAAGACACATTCCACCCCAGTGGGGGGTCCCATACCACTCATGGATCTGAATCAGATGGACACTCACATGGGAGTCAAGAAGGTGGAGCAAACACAACCTCTGGTCCTATAAGGACACCCCAAATTCCAGAATGGCTGATCATCTTGGCATCCCTCTTGGCCTTGGCTTTGATTCTTGCAGTTTGCATTGCAGTCAACAGTCGAAGAAGGTGTGGGCAGAAGAAAAAGCTAGTGATCAACAGTGGCAATGGAGCTGTGGAGGACAGAAAGCCAAGTGGACTCAACGGAGAGGCCAGCAAGTCTCAGGAAATGGTGCATTTGGTGAACAAGGAGTCGTCAGAAACTCCAGACCAGTTTATGACAGCTGATGAGACAAGGAACCTGCAGAATGTGGACATGAAGATTGGGGTGTAACACCTACACCATTATCTTGGAAAGAAACAACCGTTGGAAACATAACCATTACAGGGAGCTGGGACACTTAACAGATGCAATGTGCTACTGATTGTTTCATTGCGAATCTTTTTTAGCATAAAATTTTCTACTCTTTTTGTTTTTTGTGTTTTGTTCTTTAAAGTCAGGTCCAATTTGTAAAAACAGCATTGCTTTGTAAATTAGGGCCCAATTAATAATCAGCAAGAATTTGATCGTTCAGTTCCACTTGGAGGCCTTCATCCTCGGGTGTGCTATGGATGGCTTCTAACAAAAACTACACATATGTATTCCTGATCGCCAACCTTTCCCCCACCAGCTAAGGACATTTCCCAGGGTTAATAGGGCCTGGTCCCTGGGAGGAAATTTGAATGGGTCCATTTTGCCCTTCCATAGCCTAATCCCTGGGCATTGCTTTCCACTGAGGTTGGGGTGTACTAGTTACACATCTTCAACAGACCCCCTCTAGAAATTTTTCAGATGCTTCTGGGAGACACCAAAGGGTGAAGCTATTTATCTGTAGTAAACTATTTATCTGTGTTTTTGAAATATTAAACCCTGGATCAGTCCTTTGATCAGTATAATTTTTTAAAGTTACTTTGTCAGAGGCACAAAAGGGTTTAAACTGATTCATAATAAATATCTGTACTTCTTCGATCTTC = SEQUENCE_ID=M67454/FAS P3_COMMENT=Human Fas antigen (fas) mRNA, complete cds. CDS_195..1202 SEQUENCE_START_CODON_POSITION=194 SEQUENCE_TEMPLATE=GACGCTTCTGGGGAGTGAGGGAAGCGGTTTACGAGTGACTTGGCTGGAGCCTCAGGGGCGGGCACTGGCACGGAACACACCCTGAGGCCAGCCCTGGCTGCCCAGGCGGAGCTGCCTCTTCTCCCGCGGGTTGGTGGACCCGCTCAGTACGGAGTTGGGGAAGCTCTTTCACTTCGGAGGATTGCTCAACAACCATGCTGGGCATCTGGACCCTCCTACCTCTGGTTCTTACGTCTGTTGCTAGATTATCGTCCAAAAGTGTTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCCTGCATCATGATGGCCAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGGGATGAACCAGACTGCGTGCCCTGCCAAGAAGGGAAGGAGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGACATGGCTTAGAAGTGGAAATAAACTGCACCCGGACCCAGAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGACCCTTGCACCAAATGTGAACATGGAATCATCAAGGAATGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGTGAAGAGAAAGGAAGTACAGAAAACATGCAGAAAGCACAGAAAGGAAAACCAAGGTTCTCATGAATCTCCAACCTTAAATCCTGAAACAGTGGCAATAAATTTATCTGATGTTGACTTGAGTAAATATATCACCACTATTGCTGGAGTCATGACACTAAGTCAAGTTAAAGGCTTTGTTCGAAAGAATGGTGTCAATGAAGCCAAAATAGATGAGATCAAGAATGACAATGTCCAAGACACAGCAGAACAGAAAGTTCAACTGCTTCGTAATTGGCATCAACTTCATGGAAAGAAAGAAGCGTATGACACATTGATTAAAGATCTCAAAAAAGCCAATCTTTGTACTCTTGCAGAGAAAATTCAGACTATCATCCTCAAGGACATTACTAGTGACTCAGAAAATTCAAACTTCAGAAATGAAATCCAAAGCTTGGTCTAGAGTGAAAAACAACAAATTCAGTTCTGAGTATATGCAATTAGTGTTTGAAAAGATTCTTAATAGCTGGCTGTAAATACTGCTTGGTTTTTTACTGGGTACATTTTATCATTTATTAGCGCTGAAGAGCCAACATATTTGTAGATTTTTAATATCTCATGATTCTGCCTCCAAGGATGTTTAAAATCTAGTTGGGAAAACAAACTTCATCAAGAGTAAATGCAGTGGCATGCTAAGTACCCAAATAGGAGTGTATGCAGAGGATGAAAGATTAAGATTATGCTCTGGCATCTAACATATGATTCTGTAGTATGAATGTAATCAGTGTATGTTAGTACAAATGTCTATCCACAGGCTAACCCCACTCTATGAATCAATAGAAGAAGCTATGACCTTTTGCTGAAATATCAGTTACTGAACAGGCAGGCCACTTTGCCTCTAAATTACCTCTGATAATTCTAGAGATTTTACCATATTTCTAAACTTTGTTTATAACTCTGAGAAGATCATATTTATGTAAAGTATATGTATTTGAGTGCAGAATTTAAATAAGGCTCTACCTCAAAGACCTTTGCACAGTTTATTGGTGTCATATTATACAATATTTCAATTGTGAATTCACATAGAAAACATTAAATTATAATGTTTGACTATTATATATGTGTATGCATTTTACTGGCTCAAAACTACCTACTTCTTTCTCAGGCATCAAAAGCATTTTGAGCAGGAGAGTATTACTAGAGCTTTGCCACCTCTCCATTTTTGCCTTGGTGCTCATCTTAATGGCCTAATGCACCCCCAAACATGGAAATATCACCAAAAAATACTTAATAGTCCACCAAAAGGCAAGACTGCCCTTAGAAATTCTAGCCTGGTTTGGAGATACTAACTGCTCTCAGAGAAAGTAGCTTTGTGACATGTCATGAACCCATGTTTGCAATCAAAGATGATAAAATAGATTCTTATTTTTCCCCCACCCCCGAAAATGTTCAATAATGTCCCATGTAAAACCTGCTACAAATGGCAGCTTATACATAGCAATGGTAAAATCATCATCTGGATTTAGGAATTGCTCTTGTCATACCCTCAAGTTTCTAAGATTTAAGATTCTCCTTACTACTATCCTACGTTTAAATATCTTTGAAAGTTTGTATTAAATGTGAATTTTAAGAAATAATATTTATATTTCTGTAAATGTAAACTGTGAAGATAGTTATAAACTGAAGCAGATACCTGGAACCACCTAAAGAACTTCCATTTATGGAGGATTTTTTTGCCCCTTGTGTTTGGAATTATAAAATATAGGTAAAAGTACGTAATTAAATAATGTTTTTG = SEQUENCE_ID=U33760/SKP1 P3_COMMENT=Human cyclin A/CDK2-associated p19 (Skp1) mRNA, complete cds. CDS_94..585 SEQUENCE_START_CODON_POSITION=93 SEQUENCE_TEMPLATE=AGGATCCTCGAGGCCACGAAGGCCCTCTCCCTTCGCAAACGCCTCCCGGCTCTCGTAAGCCTCCCGCCGGCCGTCTCCTTAACACCGAACACCATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAGAGATATTTGAAGTTGATGTGGAAATTGCCAAACAATCTGTAACTATTAAGACCATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACCCAGTTCCTCTACCAAATGTGAATGCAGCAATATTAAAAAAGGTCATTCAGTGGTGCACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAGAACAAAGAAAAGCGGACAGATGATATCCCTGTTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTTTGCTTGATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTGAGGAGATTCGCAAGACCTTCAATATCAAAAATGACTTTACTGAAGAGGAGGAAGCCCAGGTACGCAAAGAGAACCAGTGGTGTGAAGAGAAGTGAAATGTTGTGCCTGACACTGTAACACTGTAAGGAT = SEQUENCE_ID=X80343/P35 P3_COMMENT=H.sapiens p35 mRNA for regulatory subunit of cdk5 kinase. CDS_98..1021 SEQUENCE_START_CODON_POSITION=97 SEQUENCE_TEMPLATE=AAACTCAGAATTTTCGCGGGCTCGGTGAGCGGTTTTATCCCTCCGGCCGGCAGGCTGGGCGCAGGGGGCGAGCCCCCGCCCGGCGCGCAGCAGCACCATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGCTGTTTGAGGATGGCGCGGCCACCGTGGGCCACTATACGGCCGTACAGAACAGCAAGAACGCCAAGGACAAGAACCTGAAGCGCCACTCCATCATCTCCGTGCTGCCTTGGAAGAGAATCGTGGCCGTGTCGGCCAAGAAGAAGAACTCCAAGAAGGTGCAGCCTAACAGCAGCTACCAGAACAACATCACGCACCTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCAACCTGTCCACATTCGCCCAGCCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGGGTTCCCAGACCGGGGGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAACGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGTTGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACACAGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTAGGCCTGGATCGGTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATTTTTAAGAATTTATTATTAAATCAGTTTTGTGTACAG = SEQUENCE_ID=L23959/DP1 P3_COMMENT=Homo sapiens E2F-related transcription factor (DP-1) mRNA, complete cds. CDS_38..1270 SEQUENCE_START_CODON_POSITION=37 SEQUENCE_TEMPLATE=GGAATTCCGTAGCTATTGATTTCCCGGATCTGGTAACATGGCAAAAGATGCCGGTCTAATTGAAGCCAACGGAGAACTCAAGGTCTTCATAGACCAGAACCTTAGTCCCGGGAAAGGCGTGGTGTCCCTCGTGGCCGTTCACCCCTCCACCGTCAACCCGCTCGGGAAGCAGCTCTTGCCAAAAACCTTTGGACAGTCCAATGTCAACATTGCCCAGCAAGTGGTAATTGGTACGCCTCAGAGACCGGCAGCGTCAAACACCCTGGTGGTAGGAAGCCCACACACCCCCAGCACTCACTTTGCCTCTCAGAACCAGCCTTCCGACTCCTCACCTTGGTCTGCCGGGAAGCGCAACAGGAAAGGAGAGAAGAATGGCAAGGGCCTACGGCATTTCTCCATGAAGGTCTGCGAGAAGGTGCAGAGGAAAGGGACCACTTCCTACAACGAAGTGGCAGACGAGCTGGTTGCGGAGTTCAGTGCTGCCGACAACCACATCTTACCAAACGAGTCAGCTTATGACCAGAAAAACATAAGACGGCGCGTCTACGATGCCTTAAACGTGCTAATGGCCATGAACATCATCTCCAAGGAGAAGAAGGAGATCAAGTGGATTGGTCTGCCCACCAACTCGGCTCAGGAATGTCAGAACTTAGAGGTGGAAAGACAGAGGAGACTTGAAAGAATAAAACAGAAACAGTCTCAACTTCAAGAACTTATTCTACAGCAAATTGCCTTCAAGAACCTGGTGCAGAGAAACCGGCATGCGGAGCAGCAGGCCAGCCGGCCACCGCCACCCAACTCAGTCATCCACCTGCCCTTCATCATCGTCAACACCAGCAAGAAGACGGTCATCGACTGCAGCATCTCCAATGACAAATTTGAGTATCTGTTTAATTTTGACAACACATTTGAAATCCACGATGACATAGAAGTGCTGAAGCGGATGGGCATGGCTTGCGGGCTGGAGTCGGGGAGCTGCTCTGCCGAAGACCTTAAAATGGCCAGAAGTCTGGTCCCCAAGGCTCTGGAGCCATACGTGACAGAAATGGCTCAGGGAACTGTTGGAGGCGTGTTCATCACGACGGCAGGTTCCACGTCTAACGGCACAAGGTTCTCTGCCAGTGACCTGACCAACGGTGCAGATGGGATGCTGGCCACAAGCTCCAATGGGTCTCAGTACAGCGGCTCCAGGGTGGAGACTCCGGTGTCCTACGTCGGGGAGGACGACGAGGAGGACGATGACTTCAACGAGAATGACGAGGACGACTGACGTCCTCCCCACTTCAGATTCGGCTTCAGGAAAACGTTTAGCGAAAAGAAACTTTTTTTTTAATGTGGGTTTTCTGTTTCCTTTTGGCCTAGTCCCAAGAAGATATTGGTAAGCTATTGAATTTAGATATGCACCTCTGATAAGCAAGGATTGTTTCCCGTAGATTAGGA = SEQUENCE_ID=X98032/E6AP P3_COMMENT=H.sapiens mRNA for E6-AP isoform-I. CDS_1..2559 SEQUENCE_START_CODON_POSITION=0 SEQUENCE_TEMPLATE=ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAGAACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAGAAAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAAATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTGGTACAGAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTCAAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGGTGATAGCTCACAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATGTGTCTGTGGATATTGATGCCATTAGAAGGGTCTACACCAGATTGCTCTCTAATGAAAAAATTGAAACTGCCTTTCTCAATGCACTTGTATATTTGTCACCTAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGATCCTAATTATCTGAATTTGTTCATTATCGGAATGGAGAATAGAAATCTCCACAGTCCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAAGCTACCCCTTGCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACAATGCAGACCAGATTCGGAGAATGATGGAGACATTTCAGCAACTTATTACTTATAAAGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTTTACTATGCAAATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAAGAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGAAGAAAGAAGAAACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAACTTGGTGTTAAAACCCTGGATTGTCGAAAACCACTTATCCCTTTTGAAGAGTTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGATTATACTTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGAATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCAAGGACAGCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATATCATAGATGATGCACTTGTCCGGCTAGAGATGATCGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAATCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTGTTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGATTGGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTTCGTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAAGATTTATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAAAATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTTTATTCTGACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGGCTTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAAAGTACTTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCAGGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACAGATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTGTGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTGCTTTAATGTGCTTTTACTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATCACGTATGCCAAAGGATTTGGCATGCTGTAA = SEQUENCE_ID=L22005/HCDC34 P3_COMMENT=Human ubiquitin conjugating enzyme mRNA, partial cds. CDS_<1..897 SEQUENCE_START_CODON_POSITION=-3 SEQUENCE_TEMPLATE=ATTGCGGCGGCGCCAGAGCTGCTGGAGCGCTCGGGGTCCCCGGGCGGCGGCGGCGGCGCAGAGGAGGAGGCAGGCGGCGGCCCCGGTGGCTCCCCCCCGGACGGTGCGCGGCCCGGCCCGTCTCGCGAACTCGCGGTGGTCGCGCGGCCCCGCGCTGCTCCGACCCCGGGCCCCTCCGCCGCCGCCATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATTTTCGGGCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCCCACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACGAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCCGACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGAGGAGTCCTGACACCACCAGAATAAACTTGCCGAGTTTACCTCACTAGGGCCGGACCCGTGGCTCCTTAGACGACAGACTACCTCACGGAGGTTTTGTGCTGGTCCCCGTCTCCTCTGGTTGTTTCGTTTTGGCTTTTTCTCCCTCCCCATGTCTGTTCTGGGTTTTCACGTGCTTCAGAGAAGAGGGGCTGCCCCACCGCCACTCACGTCACTCGGGGCTCGGTGGACGGGCCCAGGGTGGGAGCGGCCGGCCCACCTGTCCCCTCGGGAGGGGAGCTGAGCCCGACTTCTACCGGGGTCCCCCAGCTTCCGGACTGGCCGCACCCCGGAGGAGCCACGGGGGCGCTGCTGGGAACGTGGGCGGGGGGCCGTTTCCTGACACTACCAGCCTGGGAGGCCCAGGTGTAGCGGTCCGAGGGGCCCGGTCCTGCCTGTCAGCTCCAGGTCCTGGAGCCACGTCCAGCACTGAGTGGACGGATTCACCAAT = SEQUENCE_ID=M24194/MHC/RACK1 P3_COMMENT=Human MHC protein homologous to chicken B complex protein mRNA, complete cds. CDS_96..1049 SEQUENCE_START_CODON_POSITION=95 SEQUENCE_TEMPLATE=CTGCAAGGCGGCGGCAGGAGAGGTTGTGGTGCTAGTTTCTCTAAGCCATCCAGTGCCATCCTCGTCGCTGCAGCGACACCGCTCTCGCCGCCGCCATGACTGAGCAGATGACCCTTCGTGGCACCCTCAAGGGCCACAACGGCTGGGTAACCCAGATCGCTACTACCCCGCAGTTCCCGGACATGATCCTCTCCGCCTCTCGAGATAAGACCATCATCATGTGGAAACTGACCAGGGATGAGACCAACTATGGAATTCCACAGCGTGCTCTGCGGGGTCACTCCCACTTTGTTAGTGATGTGGTTATCTCCTCAGATGGCCAGTTTGCCCTCTCAGGCTCCTGGGATGGAACCCTGCGCCTCTGGGATCTCACAACGGGCACCACCACGAGGCGATTTGTGGGCCATACCAAGGATGTGCTGAGTGTGGCCTTCTCCTCTGACAACCGGCAGATTGTCTCTGGATCTCGAGATAAAACCATCAAGCTATGGAATACCCTGGGTGTGTGCAAATACACTGTCCAGGATGAGAGCCACTCAGAGTGGGTGTCTTGTGTCCGCTTCTCGCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTGGCTAACTGCAAGCTGAAGACCAACCACATTGGCCACACAGGCTATCTGAACACGGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGAGGCAAGGATGGCCAGGCCATGTTATGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACATCATCAACGCCCTGTGCTTCAGCCCTAACCGCTACTGGCTGTGTGCTGCCACAGGCCCCAGCATCAAGATCTGGGATTTAGAGGGAAAGATCATTGTAGATGAACTGAAGCAAGAAGTTATCAGTACCAGCAGCAAGGCAGAACCACCCCAGTGCACTTCCCTGGCCTGGTCTGCTGATGGCCAGACTCTGTTTGCTGGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAAAAAATGGCTTTTC = SEQUENCE_ID=X66357/CDK3 P3_COMMENT=H.sapiens mRNA cdk3 for serine/threonine protein kinase. CDS_89..1006 SEQUENCE_START_CODON_POSITION=88 SEQUENCE_TEMPLATE=CCACATGGAAGCTGGAGGAGCAACCGGGAGCGCTGGGCTGGGGTGCAAATTGCCCAGTGCCTTCTGTTTCCCAGGCAGCTCTGTGGCCATGGATATGTTCCAGAAGGTAGAGAAGATCGGAGAGGGCACCTATGGGGTGGTGTACAAGGCCAAGAACAGGGAGACAGGGCAGCTGGTGGCCCTGAAGAAGATCAGACTGGATTTGGAGATGGAGGGGGTCCCAAGCACTGCCATCAGGGAGATCTCGCTGCTCAAGGAACTGAAGCACCCCAACATCGTCCGACTGCTGGACGTGGTGCACAACGAGAGGAAGCTCTATCTGGTGTTTGAGTTCCTCAGCCAGGACCTGAAGAAGTACATGGACTCCACCCCAGGCTCAGAGCTCCCCCTGCACCTCATCAAGAGCTACCTCTTCCAGCTGCTGCAGGGGGTGAGTTTCTGCCACTCACATCGGGTCATCCACCGAGACCTGAAGCCCCAGAACCTGCTCATCAATGAGTTGGGTGCCATCAAGCTGGCTGACTTCGGCCTGGCTCGCGCCTTCGGGGTGCCCCTGCGCACCTACACCCATGAGGTGGTGACACTGTGGTATCGCGCCCCCGAGATTCTCTTGGGCAGCAAGTTCTATACCACAGCTGTGGATATCTGGAGCATTGGTTGCATCTTTGCAGAGATGGTGACTCGAAAAGCCCTGTTTCCTGGTGACTCTGAGATTGACCAGCTCTTTCGTATCTTTCGTATGCTGGGGACACCCAGCGAAGACACATGGCCCGGGGTCACCCAGCTGCCTGACTATAAGGGCAGCTTCCCTAAGTGGACCAGGAAGGGACTGGAAGAGATTGTGCCCAATCTGGAGCCAGAGGGCAGGGACCTGCTCATGCAACTCCTGCAGTATGACCCCAGCCAGCGGATCACAGCCAAGACTGCCCTGGCCCACCCGTACTTCTCATCCCCTGAGCCCTCCCCAGCTGCCCGCCAGTATGTGCTGCAGCGATTCCGCCATTGAGAATGTCAAGGCCACACTCAGATCCTTTCTCGAGCAGCAGCTGCTGCCCCAGCTGCCTCCTACCCATTGCCAAGAGAGGATGCATCTGGGGAGAGCAAAGCACTAAGGAATTCAGCATCAGCCTGCAGAGGGCTGAGTCTGGGTTAGTCCTGCCC = SEQUENCE_ID=X66364/CDK5 P3_COMMENT=H.sapiens mRNA PSSALRE for serine/threonine protein kinase. CDS_25..903 SEQUENCE_START_CODON_POSITION=24 SEQUENCE_TEMPLATE=CGCAGGGGTCCCCCGGCCGCCGCGATGCAGAAATACGAGAAACTGGAAAAGATTGGGGAAGGCACCTACGGAACTGTGTTCAAGGCCAAAAACCGGGAGACTCATGAGATCGTGGCTCTGAAACGGGTGAGGCTGGATGACGATGATGAGGGTGTGCCGAGTTCCGCCCTCCGGGAGATCTGCCTACTCAAGGAGCTGAAGCACAAGAACATCGTCAGGCTTCATGACGTCCTGCACAGCGACAAGAAGCTGACTTTGGTTTTTGAATTCTGTGACCAGGACCTGAAGAAGTATTTTGACAGTTGCAATGGTGACCTCGATCCTGAGATTGTAAAGTCATTCCTCTTCCAGCTACTAAAAGGGCTGGGATTCTGTCATAGCCGCAATGTGCTACACAGGGACCTGAAGCCCCAGAACCTGCTAATAAACAGGAATGGGGAGCTGAAATTGGCTGATTTTGGCCTGGCTCGAGCCTTTGGGATTCCCGTCCGCTGTTACTCAGCTGAGGTGGTCACACTGTGGTACCGCCCACCGGATGTCCTCTTTGGGGCCAAGCTGTACTCCACGTCCATCGACATGTGGTCAGCCGGCTGCATCTTTGCAGAGCTGGCCAATGCTGGGCGGCCTCTTTTTCCCGGCAATGATGTCGATGACCAGTTGAAGAGGATCTTCCGACTGCTGGGGACGCCCACCGAGGAGCAGTGGCCCTCTATGACCAAGCTGCCAGACTATAAGCCCTATCCGATGTACCCGGCCACAACATCCCTGGTGAACGTCGTGCCCAAACTCAATGCCACAGGGAGGGATCTGCTGCAGAACCTTCTGAAGTGTAACCCTGTCCAGCGTATCTCAGCAGAAGAGGCCCTGCAGCACCCCTACTTCTCCGACTTCTGTCCGCCCTAGGCCCCGGGACCCCCGCCTCCAGGCTGGGCCTGGCCTATTTAAGCCCCCTCTTGAGAGGGTGAGACAGTGGGGGTGCCTGG = SEQUENCE_ID=L34673/HIP116 P3_COMMENT=Human ATPase, DNA-binding protein (HIP116) mRNA, 3' end. CDS_178..3207 SEQUENCE_START_CODON_POSITION=177 SEQUENCE_TEMPLATE=ATTCCCGGGGTCTGACTGGACTCGCGGCGACTTACCTTTCAGTCGTGCGCTCCTGATCCGGCGCTCGGAATTTGTCCCCGGCTTCAGGGCTGCGGGGCCTGGAAGGAGGCGTATCGAGGCGGCTCGAAAACGATCCAGGGGAGCCGAGGCGCTCCTCTTGTCATCCCACTCAGCGCCATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAGTTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCATATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTTGAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAAGTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATTTGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTGGGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAGAAGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGGAATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGTCTACGTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGCAGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGCAGGATCTTCTAAGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTTCCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATAAAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCCAAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCATAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGCGTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGAAAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCTCAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATGAAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCTTTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAGAATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTCTTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGGTGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTCTGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAGCAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAATGAAATCAGAACATTAATTGACTTATAATTTGTGGGATTTTAGTAAGAAGACTACTATATGTGAGAGGCGTGATATCTGGATGGAAGTTGGGCTGGATGATCTCCAAAGTCGTTTCAACTCTTAAAGACATCTTAATCCTGAATGTAAACAATTGTTATGTGTTTAGAATCAGAATTTGATTTTGAACTTGAGTAATTCATCCTTACAGCTATCTGTAGAATTAGTCATCTTTTTTCTT = primer3-2.3.6/test/primer_thal_max_seq_error_input0000664000077300007730000005014712231156245021442 0ustar ioanaioanaP3_COMMENT=Test error in alignment to sequence longer than THAL_MAX_SEQ PRIMER_TASK=pick_detection_primers SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagaggtatgtattttctcattttctgccaactgtggttggcacagatggtttgaacttctgtcacatccgttgtaactttgataagtctgaaattccgcagtttgtagattactggtaaattccattataaatgtttaatgtgatttggtgattcttatcaaaagtacttgtataagtatgcgagttagataaaaaaaattatgaccatcttgttctcgtggaaatggactctgataattcataaagtctagccagtgattgtaacaaccaggctttgaacttggtacttccaatcaacttgaccttcaccagacctcattgaccacttgagtcgaaccctttaatttcagttagagtatatttaaatgctaagttactctattatttttcaaagtatatacatggtataaattttgaagttttatgtagttattgtttactttgcagatcaaaatggacatttttttcctttgtattctaggtaagtaacattgattatctcaattttcatttttgaatgatttatagaagaagtaaatattgcttcatataatttggttatatttttctaactttcattttctttttatttttccattcttgcagaacaggtttgtcttttgctattaagatgattatttgttagcttgttcacaaaaatatgagaatggacaaaaggtcaatgcttcctgtgagcttaaatttggttcaatataagcaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatggtaatatttttaaacattcatattctaagttcttattaaaaatatttcttttaacctatcttatgatataagtatttatttcagtatttgagagagcttgcgaaaatagcttataacatgtttgtttcattaaactgtatttatttcattaaatagtttatacttgctgatttttgtttatgttattggtgaagcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgaggtacgaaactacatgaatttgtttaatagagtgtactttgattttagttttgaacaagttctataaaatattttcaaaaaacttttattttttgtcataacttggaaagaaagtaaagccatttttttttccttcacgttttcattgatttcctctcatgcaacttattgtatgcagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtataccatccttttaaaaatcttaagccatatataatatatttaggagatataatcatttatttttatatatggtttgaagaatcatcgtttaactacaaagcaaataaccagtgttagttttgagaacataagaactctataactatcaagcaaaacataatctgtagtagctgtttacaattatctgtcctacacagttagcgaataatttgaaacacactgcagaacattatttgtatgtacttcttgattttgtacatgtttgtatactttttgtataatcagttttgtatttgttctagatattactctgaatttgcctaaattttatgaacaatgtaggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtaagattaacaaaaatgtgctaatatttttatgtgattttacaatattgtcaaacagtcattaatgatggttagatgatttcaggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgcaatttgaccaatcttaatgatctatattataaattttaatttatcacttcttcttttacattaattaactctatgaatggttttgaattcaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagagtatgattcgtttgtattaaattttgagtttaatattagtacaaaaagtacaacaaaaattcagtgattcattcacatttcacaatacatatgtcactttgttatattataaaatgggatatgaccagatgattgtacaattttttttataacaaatgatatttgtataacccttttagtatgtccatggattataaactatcttcaactttcttaattgtagaaaacatgtttgtttattagctgttttttttctctgttgcagatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattggtaggtctagataccatatttattaagaaaacactcatttcatgtatatttttagtaaaatatttttaagttagtaattatgtacattttaaattcagtaaactgaatgcattcacttaaaccagaacaaaagttatccttgattattttgtattgcagtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaaggtgggaagcattttttcttagcaaaaaattgaatgttatttctttttcttctcaatttgcattatataccaacaaaaaaaaaatgcatatttatgtggtatagcctttcaaatcattgtagtacataagcaaagttcatgttattaaaatataattaaatgtatgcaaaagtgtatagtttgtaaagttactaaactcatttgttttagcactagattttgtcattgaacataacttaagatatgtgaatatttgaattgcagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgattcttgagaaagataatgagaactctgtcaccacaatattgtattggtgagatcatataagatttgatg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PRIMER_MAX_SELF_END_TH=47.00 PRIMER_INTERNAL_MAX_SELF_END_TH=47.00 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=-1.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_NUM_RETURN=5 PRIMER_PAIR_MAX_COMPL_END_TH=47.00 PRIMER_MAX_SELF_ANY_TH=47.00 PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.00 PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.0 PRIMER_MAX_HAIRPIN_TH=47.00 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_FIRST_BASE_INDEX=1 PRIMER_PRODUCT_SIZE_RANGE=501-600 601-700 401-500 701-850 851-1000 1000-1500 1500-3000 = primer3-2.3.6/test/primer_tm_lc_masking_formatted_output0000664000077300007730000003323712231156245022642 0ustar ioanaioanaPRIMER PICKING RESULTS FOR 000 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 10 15.11 40.00 2.00 2.00 TCCTTGTGAT SEQUENCE SIZE: 10 INCLUDED REGION SIZE: 10 0 TCCTTGTGAT >>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR 001 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 10 22.87 40.00 2.00 2.00 TCCTTGTGAT SEQUENCE SIZE: 10 INCLUDED REGION SIZE: 10 0 TCCTTGTGAT >>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR 002 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 10 22.82 40.00 2.00 2.00 TCCTTGTGAT SEQUENCE SIZE: 10 INCLUDED REGION SIZE: 10 0 TCCTTGTGAT >>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR 100 No mispriming library specified Using 0-based sequence positions NO LEFT PRIMER FOUND 0 TCCTTGTGAt Statistics con too in in not no tm tm high high high lower sid many tar excl ok bad GC too too any 3' poly end case ered Ns get reg reg GC% clamp low high compl compl X stab end ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR 101 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 10 22.87 40.00 2.00 2.00 TCCTTGTGaT SEQUENCE SIZE: 10 INCLUDED REGION SIZE: 10 0 TCCTTGTGaT >>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high lower sid many tar excl ok bad GC too too any 3' poly end case ered Ns get reg reg GC% clamp low high compl compl X stab end ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR 102 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 10 22.82 40.00 2.00 2.00 tccttgtgat SEQUENCE SIZE: 10 INCLUDED REGION SIZE: 10 0 tccttgtgat >>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR 010 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 10 9.73 40.00 2.00 2.00 TCCTTGTGAT SEQUENCE SIZE: 10 INCLUDED REGION SIZE: 10 0 TCCTTGTGAT >>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR 011 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 10 20.94 50.00 2.00 0.00 TCCTTGTGAG SEQUENCE SIZE: 10 INCLUDED REGION SIZE: 10 0 TCCTTGTGAG >>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR 012 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 10 26.08 60.00 2.00 0.00 GCCTTGTGAG SEQUENCE SIZE: 10 INCLUDED REGION SIZE: 10 0 GCCTTGTGAG >>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR 110 No mispriming library specified Using 0-based sequence positions NO LEFT PRIMER FOUND 0 TCCTTGTGAt Statistics con too in in not no tm tm high high high lower sid many tar excl ok bad GC too too any 3' poly end case ered Ns get reg reg GC% clamp low high compl compl X stab end ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR 111 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 10 18.40 40.00 2.00 2.00 TCCTTGTGaT SEQUENCE SIZE: 10 INCLUDED REGION SIZE: 10 0 TCCTTGTGaT >>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high lower sid many tar excl ok bad GC too too any 3' poly end case ered Ns get reg reg GC% clamp low high compl compl X stab end ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR 112 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT_PRIMER 0 10 17.92 40.00 2.00 2.00 tccttgtgat SEQUENCE SIZE: 10 INCLUDED REGION SIZE: 10 0 tccttgtgat >>>>>>>>>> KEYS (in order of precedence): >>>>>> left primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR masking_001 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 20 46.14 40.00 3.00 3.00 GCCTTCATTAGAAAGTGACA RIGHT PRIMER 69 20 46.49 40.00 4.00 1.00 ATTAGTACCCAGGTGTGATT SEQUENCE SIZE: 70 INCLUDED REGION SIZE: 70 PRODUCT SIZE: 70, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00 0 GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCT >>>>>>>>>>>>>>>>>>>> <<<<<<<<<< 60 GGGTACTAAT <<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high lower sid many tar excl ok bad GC too too any 3' poly end case ered Ns get reg reg GC% clamp low high compl compl X stab end ok Left 31 30 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 31 30 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR masking_002 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 70 INCLUDED REGION SIZE: 70 0 GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNaATCACACCT 60 GGGTACTAAT Statistics con too in in not no tm tm high high high lower sid many tar excl ok bad GC too too any 3' poly end case ered Ns get reg reg GC% clamp low high compl compl X stab end ok Left 31 30 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 31 30 0 0 0 0 0 0 0 0 0 0 0 1 0 Pair Stats: considered 0, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR masking_003 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 20 46.14 40.00 3.00 3.00 GCCTTCATTAGAAAGTGACA RIGHT PRIMER 69 20 46.49 40.00 4.00 1.00 aTTAGTACCCAGGTGTGATT SEQUENCE SIZE: 70 INCLUDED REGION SIZE: 70 PRODUCT SIZE: 70, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00 0 GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCT >>>>>>>>>>>>>>>>>>>> <<<<<<<<<< 60 GGGTACTAAt <<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high lower sid many tar excl ok bad GC too too any 3' poly end case ered Ns get reg reg GC% clamp low high compl compl X stab end ok Left 31 30 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 31 30 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR masking_004 No mispriming library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 0 20 46.14 40.00 3.00 3.00 gCCTTCATTAGAAAGTGACA RIGHT PRIMER 69 20 46.49 40.00 4.00 1.00 ATTAGTACCCAGGTGTGATT SEQUENCE SIZE: 70 INCLUDED REGION SIZE: 70 PRODUCT SIZE: 70, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00 0 gCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCT >>>>>>>>>>>>>>>>>>>> <<<<<<<<<< 60 GGGTACTAAT <<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high lower sid many tar excl ok bad GC too too any 3' poly end case ered Ns get reg reg GC% clamp low high compl compl X stab end ok Left 31 30 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 31 30 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR masking_005 No mispriming library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 70 INCLUDED REGION SIZE: 70 0 GCCTTCATTAGAAAGTGACaNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCT 60 GGGTACTAAT Statistics con too in in not no tm tm high high high lower sid many tar excl ok bad GC too too any 3' poly end case ered Ns get reg reg GC% clamp low high compl compl X stab end ok Left 31 30 0 0 0 0 0 0 0 0 0 0 0 1 0 Right 31 30 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 primer3 release 2.0.0 primer3-2.3.6/test/Makefile0000664000077300007730000001252412231156245014501 0ustar ioanaioana# ====================================================================== # (c) Copyright 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009, # 2010,2011 # Whitehead Institute for Biomedical Research, Steve Rozen, # Andreas Untergasser and Helen Skaletsky # All rights reserved. # # This file is part of primer3, the libprimer3 library, the oligotm # library and the dpal library. # # Primer3 and the libraries above are free software; you can # redistribute them and/or modify them under the terms of the GNU # General Public License as published by the Free Software Foundation; # either version 2 of the License, or (at your option) any later # version. # # This software is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # # You should have received a copy of the GNU General Public License # along with this file (file gpl-2.0.txt in the source distribution); if # not, write to the Free Software Foundation, Inc., 51 Franklin St, # Fifth Floor, Boston, MA 02110-1301 USA # # THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS # "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT # LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR # A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT # OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, # SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT # LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, # DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY # THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT # (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE # OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. # ====================================================================== WINMAKE=mingw32-make # See ../src/Makefile for detailed instructions on how to use TESTOPTS # The only valid non-empty values for TESTOPTS are --valgrind and --windows TESTOPTS = test: makeexes testcmdline testtm testdpal testthal testcore echo; echo; echo "Testing completed. You must check the test output for the string FAILED"; echo; echo; testcmdline: ifeq ($(TESTOPTS),--windows) perl cmdline_test.pl $(TESTOPTS) else perl cmdline_test.pl $(TESTOPTS) endif testcore: ifeq ($(TESTOPTS),--windows) echo 'TESTING primer3_core' & perl p3test.pl $(TESTOPTS) else echo; echo; echo 'TESTING primer3_core'; echo; perl p3test.pl $(TESTOPTS) endif testdpal: ifeq ($(TESTOPTS),--windows) echo 'TESTING ALIGNMENT CALCS' & perl dpal_test.pl $(TESTOPTS) else echo; echo; echo 'TESTING ALIGNMENT CALCS'; echo; perl dpal_test.pl $(TESTOPTS); endif testthal: ifeq ($(TESTOPTS),--windows) echo 'TESTING THERMODYNAMIC ALIGNMENT CALCS' & perl thal_test.pl $(TESTOPTS) else echo; echo; echo 'TESTING THERMODYNAMIC ALIGNMENT CALCS'; echo; perl thal_test.pl $(TESTOPTS); endif testtm: ifeq ($(TESTOPTS),--windows) perl oligotm_test.pl $(TESTOPTS) else perl oligotm_test.pl $(TESTOPTS) endif makeexes: ifeq ($(TESTOPTS),--windows) cd ..\src & $(WINMAKE) else cd ../src; make endif clean: ifeq ($(TESTOPTS),--windows) echo 'CLEAN UP TEST DIRECTORIES' del /Q /F primer1_list_tmp\* del /Q /F primer1_th_list_tmp\* del /Q /F primer_list_tmp\* del /Q /F th-w-other-tasks_list_tmp\* del /Q /F primer_global_err\*.tmp primer_global_err\*.tmp2 primer_global_err\*.bak del /Q /F *.tmp *.bak *.tmp.err *~ del /Q /F hyb_probe_only.for hyb_probe_only.int del /Q /F right_only.for right_only.rev left_only.for del /Q /F pcr_primers_and_io.int pcr_primers_and_io.rev pcr_primers_and_io.for del /Q /F pcr_primers.rev pcr_primers.for del /Q /F pick_primer_list_and_file_flag_th_*.for pick_primer_list_and_file_flag_th_*.rev del /Q /F pick_primer_list_and_file_flag_th_*.int del /Q /F syntax*.for syntax*.int syntax*.rev else echo; echo; echo 'CLEAN UP TEST DIRECTORIES'; echo; echo; -rm -rf primer_list_tmp/* -rm -rf primer1_list_tmp/* -rm -rf primer1_th_list_tmp/* -rm -rf th-w-other-tasks_list_tmp/* -rm -f primer_global_err/*.tmp primer_global_err/*.tmp2 primer_global_err/*.bak -rm -f *.tmp *.bak *.tmp.err *~ -rm -f hyb_probe_only.for hyb_probe_only.int -rm -f right_only.for right_only.rev left_only.for -rm -f pcr_primers_and_io.int pcr_primers_and_io.rev pcr_primers_and_io.for -rm -f pcr_primers.rev pcr_primers.for -rm -f pick_primer_list_and_file_flag_th_[1-4].for pick_primer_list_and_file_flag_th_[1-4].rev -rm -f pick_primer_list_and_file_flag_th_[1-4].int -rm -f syntax[0-1][0-9].for syntax[0-1][0-9].int syntax[0-1][0-9].rev -rm -f oligotm.*.valg # valgrind output from oligotm_test.pl --valgrind -rm -f ntdpal.*.valg # valgrind output from dpal_test.pl --valgrind -rm -f ntthal.*.valg # valgrind output from thal_test.pl --valgrind -rm -f oligotm.*.valg.* # valgrind output from oligotm_test.pl --valgrind -rm -f ntdpal.*.valg.* # valgrind output from dpal_test.pl --valgrind -rm -f ntthal.*.valg.* # valgrind output from thal_test.pl --valgrind -rm -f *.vg */*.vg # valgrind output from p3test{,z}.pl --valgrind -rm -f *.vg.core.* # core files from crashed valgrind test -rm -f ./core # in case we crashed and left a core file endif primer3-2.3.6/test/primer_quality_boundary_input0000664000077300007730000002340412231156245021153 0ustar ioanaioanaSEQUENCE_ID=quality_test1 PRIMER_EXPLAIN_FLAG=1 P3_COMMENT=quality scores out of range PRIMER_MIN_TM=0 PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 PRIMER_MAX_TM=100 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=20 PRIMER_PAIR_MAX_COMPL_ANY=20 PRIMER_PAIR_MAX_COMPL_END=20 PRIMER_PRODUCT_SIZE_RANGE=50-70 70-90 90-110 110-500 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC PRIMER_MIN_QUALITY=30 PRIMER_QUALITY_RANGE_MAX=40 SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 = SEQUENCE_ID=quality_test2 P3_COMMENT=quality scores out of range SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_MIN_QUALITY=60 PRIMER_QUALITY_RANGE_MIN=60 PRIMER_QUALITY_RANGE_MAX=65 = SEQUENCE_ID=quality_test3 P3_COMMENT=quality scores out of range SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_MIN_QUALITY=9 PRIMER_QUALITY_RANGE_MAX=49 = SEQUENCE_ID=quality_test4 P3_COMMENT=Should return exactly one pair SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC PRIMER_MIN_QUALITY=60 PRIMER_QUALITY_RANGE_MAX=70 PRIMER_MIN_SIZE=10 SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 = SEQUENCE_ID=quality_test5 P3_COMMENT=Should return exactly one pair PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 = SEQUENCE_ID=quality_test6 P3_COMMENT=Should fail because of low quality for middle oligo PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=51 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 53 54 54 54 54 50 54 54 54 54 54 51 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 = SEQUENCE_ID=quality_test7 P3_COMMENT=Should fail as there are too few score values PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=20 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 = SEQUENCE_ID=quality_test8 P3_COMMENT=Should fail as there are too many score values PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=20 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY= 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 = SEQUENCE_ID=quality_test9 P3_COMMENT=Should fail because of low end quality PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 = SEQUENCE_ID=quality_test10 P3_COMMENT=Should return exactly one pair PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 60 60 60 60 60 = SEQUENCE_ID=quality_test11 P3_COMMENT=Should fail as high quality pairs are not in the included region PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_INCLUDED_REGION=2,115 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 60 60 60 60 60 = SEQUENCE_ID=quality_test12 P3_COMMENT=Should return exactly one pair PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_INCLUDED_REGION=2,115 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 61 61 61 61 60 50 50 50 50 = SEQUENCE_ID=quality_test13 P3_COMMENT=Should return exactly one pair PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_INCLUDED_REGION=2,115 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY=60 60 60 60.33 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 61 61 61 61 60 50 50 50 50 = SEQUENCE_ID=quality_test14 P3_COMMENT=Should return exactly one pair PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MIN_QUALITY=55 PRIMER_INTERNAL_MIN_SIZE=12 PRIMER_MIN_END_QUALITY=61 SEQUENCE_INCLUDED_REGION=2,115 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC SEQUENCE_QUALITY= 60 60 60 60 60 61 61 61 61 61 61 61 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 55 55 55 55 55 55 55 55 55 55 55 55 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 61 61 61 61 61 61 61 61 61 60 50 50 50 50 = primer3-2.3.6/test/primer_internal1_formatted_output0000664000077300007730000013454512231156245021734 0ustar ioanaioanaPRIMER PICKING RESULTS FOR boundary_test_00 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 35 33 0 0 0 0 0 0 0 0 0 0 0 2 Right 35 33 0 0 0 0 0 0 0 0 0 0 0 2 Intl 60 57 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 4, unacceptable product size 1, no internal oligo 2, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_01 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 30 28 0 0 0 0 0 0 0 0 0 0 0 2 Right 30 29 0 0 0 0 0 0 0 0 0 0 0 1 Intl 60 57 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 2, unacceptable product size 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_02 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Intl 60 57 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_05 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 30 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 1 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Intl 60 57 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 4, unacceptable product size 3, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_11 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 1 NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACT 61 CACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 1 0 0 0 0 0 0 0 0 0 0 0 4 Intl 60 59 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 8, unacceptable product size 6, no internal oligo 2, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_11 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 24 60.81 31.82 12.00 11.50 CACGTCATCNNATCAAATTTGATT RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 30 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00 1 NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 4 24 60.81 31.82 12.00 11.00 ACGTCATCNNATCAAATTTGATTG RIGHT PRIMER 83 24 61.74 34.78 4.00 3.00 NGAATTGTTTGGAAATGACAGTGA INTERNAL OLIGO 30 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG PRODUCT SIZE: 80, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Intl 60 52 0 0 0 0 0 0 0 0 0 0 0 8 Pair Stats: considered 10, unacceptable product size 8, ok 2 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_12 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 24 60.81 31.82 12.00 11.50 CACGTCATCNNATCAAATTTGATT RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 30 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00 1 NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 4 24 60.81 31.82 12.00 11.00 ACGTCATCNNATCAAATTTGATTG RIGHT PRIMER 83 24 61.74 34.78 4.00 3.00 NGAATTGTTTGGAAATGACAGTGA INTERNAL OLIGO 30 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG PRODUCT SIZE: 80, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Intl 60 52 0 0 0 0 0 0 0 0 0 0 0 8 Pair Stats: considered 10, unacceptable product size 8, ok 2 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_13 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 24 54.69 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAA RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 32 25 56.20 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTNN SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 1 NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Intl 60 23 0 0 0 0 0 0 0 0 0 0 0 37 Pair Stats: considered 4, unacceptable product size 3, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_14 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 24 55.24 4.17 0.00 0.00 AAAAAAAAAAAAAAAAACAAAAAA RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 30 25 56.20 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTNN SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00 1 NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 2 0 0 0 0 0 0 0 0 0 0 0 3 Right 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Intl 60 25 0 0 0 0 0 0 0 0 0 0 0 35 Pair Stats: considered 6, unacceptable product size 5, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_15 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 24 89.31 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGG RIGHT PRIMER 82 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 33 25 55.84 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTTT SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00 1 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 2 0 0 0 0 0 0 0 0 0 0 0 3 Intl 60 25 0 0 0 0 0 0 0 0 0 0 0 35 Pair Stats: considered 6, unacceptable product size 5, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_16 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 1 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 8 0 0 0 0 0 0 0 0 0 0 0 2 Right 10 8 0 0 0 0 0 0 0 0 0 0 0 2 Intl 35 0 0 0 0 0 0 0 0 0 0 0 0 35 Pair Stats: considered 4, no internal oligo 4, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_17 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 1 NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACT 61 CACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 35 31 0 0 0 0 0 0 0 0 0 0 0 4 Right 35 31 0 0 0 0 0 0 0 0 0 0 0 4 Intl 60 0 0 0 0 29 0 0 0 0 0 0 0 31 Pair Stats: considered 16, unacceptable product size 4, no internal oligo 12, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_18 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 111 INCLUDED REGION SIZE: 111 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 11,50 1 NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGN xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 61 NNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN KEYS (in order of precedence): xxxxxx excluded region for internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 62 53 0 0 0 0 0 0 0 0 0 0 0 9 Right 62 53 0 0 0 0 0 0 0 0 0 0 0 9 Intl 87 39 0 40 0 0 0 0 0 0 0 0 0 8 Pair Stats: considered 81, unacceptable product size 49, no internal oligo 32, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_19 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 113 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 61 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT SEQUENCE SIZE: 115 INCLUDED REGION SIZE: 115 PRODUCT SIZE: 110, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 11,50 1 NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNN >>>>>>>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 61 CCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 113 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 61 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT PRODUCT SIZE: 111, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 112 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 61 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT PRODUCT SIZE: 109, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 3 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 112 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 61 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT PRODUCT SIZE: 110, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 4 LEFT PRIMER 30 24 67.92 54.17 9.00 0.00 AAGCTAAGCTTGCCCGTATCCCCT RIGHT PRIMER 113 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 61 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT PRODUCT SIZE: 84, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 66 57 0 0 0 0 0 0 0 0 0 0 0 9 Right 66 57 0 0 0 0 0 0 0 0 0 0 0 9 Intl 91 84 0 5 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 16, unacceptable product size 3, no internal oligo 8, ok 5 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_20 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 252 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 76 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG SEQUENCE SIZE: 291 INCLUDED REGION SIZE: 250 PRODUCT SIZE: 249, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 6,50 61,15 1 NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATA .. >>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 61 CGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNN xxxxxxxxxxxxxxx^^^^^^^^^^^^^^^^^^^^^^^^^ 121 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 181 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATT <<<<<<<<<<<< 241 TCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT <<<<<<<<<<<<....................................... KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 252 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 76 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG PRODUCT SIZE: 250, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 51 24 60.30 50.00 8.00 6.00 GGAGACGATACGGTAAGCTAGCTA RIGHT PRIMER 252 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 76 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG PRODUCT SIZE: 202, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00 3 LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 251 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 76 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 4 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 251 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 76 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG PRODUCT SIZE: 249, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 151 99 0 0 0 0 0 0 0 0 0 0 0 52 Right 151 148 0 0 0 0 0 0 0 0 0 0 0 3 Intl 226 175 0 47 0 0 0 0 0 0 0 0 0 4 Pair Stats: considered 14, no internal oligo 8, ok 6 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_21 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 174 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 62 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA SEQUENCE SIZE: 176 INCLUDED REGION SIZE: 176 PRODUCT SIZE: 171, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >>>>>>>>>>>>>>>>>>>>>>>> 61 GAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG ^^^^^^^^^^^^^^^^^^^^^^^^^ 121 GGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 174 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 62 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA PRODUCT SIZE: 172, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 4 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 173 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 62 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA PRODUCT SIZE: 170, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 3 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 173 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 62 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA PRODUCT SIZE: 171, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 4 LEFT PRIMER 38 24 89.31 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGG RIGHT PRIMER 174 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 62 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA PRODUCT SIZE: 137, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 77 27 0 0 0 0 0 0 0 0 0 0 0 50 Right 77 28 0 0 0 0 0 0 0 0 0 0 0 49 Intl 152 57 0 0 0 0 0 1 93 0 0 0 0 1 Pair Stats: considered 323, unacceptable product size 223, no internal oligo 87, ok 13 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_22 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Intl 60 57 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_23 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 2,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_24 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 3,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..x>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_25 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 1,3 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..x>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_26 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 30,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> x^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_27 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 31,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT .. x 61 CACTGTCATTTCCAAACAATTCNN .. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, no internal oligo 1, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_28 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 55,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT .. x 61 CACTGTCATTTCCAAACAATTCNN .. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, no internal oligo 1, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_29 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 56,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^x <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_30 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 82,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_31 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 83,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_32 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 82,2 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_33 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 82 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 31 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 82,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 61 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_34 INPUT PROBLEM: Too many elements for tag SEQUENCE_INTERNAL_EXCLUDED_REGION primer3-2.3.6/test/primer3web_v3_0_0_default_settings_output0000664000077300007730000002364612231156245023164 0ustar ioanaioanaP3_SETTINGS_FILE_USED=../primer3web_v3_0_0_default_settings.txt P3_FILE_TYPE=settings PRIMER_FIRST_BASE_INDEX=1 PRIMER_TASK=generic P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers PRIMER_MIN_THREE_PRIME_DISTANCE=3 PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY= PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00 PRIMER_PRODUCT_SIZE_RANGE=150-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 PRIMER_MAX_SELF_ANY_TH=45.00 PRIMER_MAX_SELF_END_TH=35.00 PRIMER_PAIR_MAX_COMPL_ANY_TH=45.00 PRIMER_PAIR_MAX_COMPL_END_TH=35.00 PRIMER_MAX_HAIRPIN_TH=24.00 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=40.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=70.00 PRIMER_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_MAX_SIZE=23 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=59.0 PRIMER_MAX_TM=62.0 PRIMER_PAIR_MAX_DIFF_TM=5.0 PRIMER_TM_FORMULA=1 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 PRIMER_DNTP_CONC=0.6 PRIMER_DNA_CONC=50.0 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_LOWERCASE_MASKING=0 PRIMER_MIN_GC=30.0 PRIMER_MAX_GC=70.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_POLY_X=4 PRIMER_OUTSIDE_PENALTY=0 PRIMER_GC_CLAMP=0 PRIMER_LIBERAL_BASE=1 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_PICK_ANYWAY=1 PRIMER_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_WT_SELF_END_TH=0.0 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_WT_NUM_NS=0.0 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_WT_END_QUAL=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_END_TH=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_END_QUAL=0.0 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_PRODUCT_MAX_TM=1000000.0 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_SEQUENCING_ACCURACY=20 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MAX_SELF_END=3.00 PRIMER_QUALITY_RANGE_MAX=100 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_SEQUENCING_INTERVAL=250 PRIMER_INTERNAL_SALT_DIVALENT=1.5 PRIMER_MAX_SELF_ANY=8.00 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_SEQUENCING_SPACING=500 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_MIN_END_QUALITY=0 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_SEQUENCING_LEAD=50 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_MAX_END_GC=5 PRIMER_MIN_QUALITY=0 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_WT_SELF_ANY=0.0 PRIMER_MAX_TEMPLATE_MISPRIMING=12.00 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_SELF_END=0.0 PRIMER_PRODUCT_OPT_SIZE=0 PRIMER_PRODUCT_OPT_TM=0.0 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=24.00 PRIMER_INSIDE_PENALTY=-1.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_PRODUCT_MIN_TM=-1000000.0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_WT_SELF_END=0.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_TM_LT=1.0 P3_SETTINGS_FILE_END= SEQUENCE_ID=test_settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LEFT_EXPLAIN=considered 7867, GC content failed 203, low tm 3099, high tm 1432, high hairpin stability 4, long poly-x seq 168, ok 2961 PRIMER_RIGHT_EXPLAIN=considered 7867, GC content failed 226, low tm 3298, high tm 1334, high hairpin stability 1, long poly-x seq 168, ok 2840 PRIMER_PAIR_EXPLAIN=considered 266, unacceptable product size 260, primer in pair overlaps a primer in a better pair 25, ok 6 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.046400 PRIMER_LEFT_0_PENALTY=0.005753 PRIMER_RIGHT_0_PENALTY=0.040646 PRIMER_LEFT_0_SEQUENCE=tgccatcaatgacctccact PRIMER_RIGHT_0_SEQUENCE=tgagctgttcgaggacagtt PRIMER_LEFT_0=21,20 PRIMER_RIGHT_0=214,20 PRIMER_LEFT_0_TM=59.006 PRIMER_RIGHT_0_TM=58.959 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=16.51 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=11.79 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.0000 PRIMER_RIGHT_0_END_STABILITY=3.1600 PRIMER_LEFT_0_TEMPLATE_MISPRIMING_TH=29.7518 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING_TH=16.0633 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=194 PRIMER_PAIR_0_TEMPLATE_MISPRIMING_TH=45.82 PRIMER_PAIR_1_PENALTY=0.053176 PRIMER_LEFT_1_PENALTY=0.024271 PRIMER_RIGHT_1_PENALTY=0.028905 PRIMER_LEFT_1_SEQUENCE=gacacacttgctgctttcca PRIMER_RIGHT_1_SEQUENCE=atgtggaccgttgttgttgg PRIMER_LEFT_1=610,20 PRIMER_RIGHT_1=804,20 PRIMER_LEFT_1_TM=58.976 PRIMER_RIGHT_1_TM=58.971 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=3.5300 PRIMER_RIGHT_1_END_STABILITY=3.7700 PRIMER_LEFT_1_TEMPLATE_MISPRIMING_TH=14.5725 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING_TH=26.7482 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=195 PRIMER_PAIR_1_TEMPLATE_MISPRIMING_TH=36.10 PRIMER_PAIR_2_PENALTY=0.068003 PRIMER_LEFT_2_PENALTY=0.040646 PRIMER_RIGHT_2_PENALTY=0.027357 PRIMER_LEFT_2_SEQUENCE=aactgtcctcgaacagctca PRIMER_RIGHT_2_SEQUENCE=ccagccatcaataggtgcac PRIMER_LEFT_2=195,20 PRIMER_RIGHT_2=371,20 PRIMER_LEFT_2_TM=58.959 PRIMER_RIGHT_2_TM=58.973 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY_TH=5.32 PRIMER_RIGHT_2_SELF_ANY_TH=8.80 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=8.80 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=4.2600 PRIMER_RIGHT_2_END_STABILITY=4.5700 PRIMER_LEFT_2_TEMPLATE_MISPRIMING_TH=26.5528 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING_TH=21.7248 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=177 PRIMER_PAIR_2_TEMPLATE_MISPRIMING_TH=41.37 PRIMER_PAIR_3_PENALTY=0.082329 PRIMER_LEFT_3_PENALTY=0.028905 PRIMER_RIGHT_3_PENALTY=0.053424 PRIMER_LEFT_3_SEQUENCE=ccaacaacaacggtccacat PRIMER_RIGHT_3_SEQUENCE=aaccaaagcctctccacgta PRIMER_LEFT_3=785,20 PRIMER_RIGHT_3=996,20 PRIMER_LEFT_3_TM=58.971 PRIMER_RIGHT_3_TM=58.947 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=3.2100 PRIMER_RIGHT_3_END_STABILITY=3.5700 PRIMER_LEFT_3_TEMPLATE_MISPRIMING_TH=31.8678 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING_TH=29.3884 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=212 PRIMER_PAIR_3_TEMPLATE_MISPRIMING_TH=50.80 PRIMER_PAIR_4_PENALTY=0.106163 PRIMER_LEFT_4_PENALTY=0.081892 PRIMER_RIGHT_4_PENALTY=0.024271 PRIMER_LEFT_4_SEQUENCE=caacatggagagacttgccg PRIMER_RIGHT_4_SEQUENCE=tggaaagcagcaagtgtgtc PRIMER_LEFT_4=468,20 PRIMER_RIGHT_4=629,20 PRIMER_LEFT_4_TM=58.918 PRIMER_RIGHT_4_TM=58.976 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=5.6900 PRIMER_RIGHT_4_END_STABILITY=3.6700 PRIMER_LEFT_4_TEMPLATE_MISPRIMING_TH=14.6991 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING_TH=14.5815 PRIMER_PAIR_4_COMPL_ANY_TH=1.86 PRIMER_PAIR_4_COMPL_END_TH=13.09 PRIMER_PAIR_4_PRODUCT_SIZE=162 PRIMER_PAIR_4_TEMPLATE_MISPRIMING_TH=29.28 = primer3-2.3.6/test/primer3_v1_1_4_default_settings_input0000664000077300007730000000304612231156245022260 0ustar ioanaioanaSEQUENCE_ID=test_settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag = primer3-2.3.6/test/repeat_lib60000664000077300007730000001600112231156245015152 0ustar ioanaioana>ALU--repbase Human ALU interspersed repetitive sequence - a consensus * 2.1 GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA >MIR--repbase mammalian-wide interspersed repeat (MIR) - a consensus * 100.1 GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTC GAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTG TAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCG CCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA >MIR2--repbase Repetitive element - a consensus * 1 TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT >L1--repbase Human L1 interspersed repetitive sequence - full length copy GGCGGTGGAGCCAAGATGACCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGAC CATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACC ACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAA AA primer3-2.3.6/test/primer_gc_end_output0000664000077300007730000010350612231156245017202 0ustar ioanaioanaSEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.013026 PRIMER_LEFT_0_PENALTY=0.006937 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_0=198,20 PRIMER_RIGHT_0=315,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.006 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=118 PRIMER_PAIR_1_PENALTY=0.032388 PRIMER_LEFT_1_PENALTY=0.032351 PRIMER_RIGHT_1_PENALTY=0.000037 PRIMER_LEFT_1_SEQUENCE=gacacacttgctgctttcca PRIMER_RIGHT_1_SEQUENCE=gtttcttgtacggtggcgat PRIMER_LEFT_1=609,20 PRIMER_RIGHT_1=868,20 PRIMER_LEFT_1_TM=60.032 PRIMER_RIGHT_1_TM=60.000 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=9.8000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=260 PRIMER_PAIR_2_PENALTY=0.037980 PRIMER_LEFT_2_PENALTY=0.031043 PRIMER_RIGHT_2_PENALTY=0.006937 PRIMER_LEFT_2_SEQUENCE=actggtctcccatcgtcaac PRIMER_RIGHT_2_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_2=106,20 PRIMER_RIGHT_2=217,20 PRIMER_LEFT_2_TM=59.969 PRIMER_RIGHT_2_TM=59.993 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=112 PRIMER_PAIR_3_PENALTY=0.052224 PRIMER_LEFT_3_PENALTY=0.000037 PRIMER_RIGHT_3_PENALTY=0.052187 PRIMER_LEFT_3_SEQUENCE=atcgccaccgtacaagaaac PRIMER_RIGHT_3_SEQUENCE=cggtgcacggtaactaaggt PRIMER_LEFT_3=849,20 PRIMER_RIGHT_3=1040,20 PRIMER_LEFT_3_TM=60.000 PRIMER_RIGHT_3_TM=60.052 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=192 PRIMER_PAIR_4_PENALTY=0.064713 PRIMER_LEFT_4_PENALTY=0.006089 PRIMER_RIGHT_4_PENALTY=0.058624 PRIMER_LEFT_4_SEQUENCE=aacaacggcaataaaaacgc PRIMER_RIGHT_4_SEQUENCE=agccggatcaatatcactcg PRIMER_LEFT_4=296,20 PRIMER_RIGHT_4=433,20 PRIMER_LEFT_4_TM=60.006 PRIMER_RIGHT_4_TM=60.059 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.9000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=138 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=5 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.013026 PRIMER_LEFT_0_PENALTY=0.006937 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_0=198,20 PRIMER_RIGHT_0=315,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.006 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=118 PRIMER_PAIR_1_PENALTY=0.032388 PRIMER_LEFT_1_PENALTY=0.032351 PRIMER_RIGHT_1_PENALTY=0.000037 PRIMER_LEFT_1_SEQUENCE=gacacacttgctgctttcca PRIMER_RIGHT_1_SEQUENCE=gtttcttgtacggtggcgat PRIMER_LEFT_1=609,20 PRIMER_RIGHT_1=868,20 PRIMER_LEFT_1_TM=60.032 PRIMER_RIGHT_1_TM=60.000 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=9.8000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=260 PRIMER_PAIR_2_PENALTY=0.037980 PRIMER_LEFT_2_PENALTY=0.031043 PRIMER_RIGHT_2_PENALTY=0.006937 PRIMER_LEFT_2_SEQUENCE=actggtctcccatcgtcaac PRIMER_RIGHT_2_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_2=106,20 PRIMER_RIGHT_2=217,20 PRIMER_LEFT_2_TM=59.969 PRIMER_RIGHT_2_TM=59.993 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=112 PRIMER_PAIR_3_PENALTY=0.052224 PRIMER_LEFT_3_PENALTY=0.000037 PRIMER_RIGHT_3_PENALTY=0.052187 PRIMER_LEFT_3_SEQUENCE=atcgccaccgtacaagaaac PRIMER_RIGHT_3_SEQUENCE=cggtgcacggtaactaaggt PRIMER_LEFT_3=849,20 PRIMER_RIGHT_3=1040,20 PRIMER_LEFT_3_TM=60.000 PRIMER_RIGHT_3_TM=60.052 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=192 PRIMER_PAIR_4_PENALTY=0.064713 PRIMER_LEFT_4_PENALTY=0.006089 PRIMER_RIGHT_4_PENALTY=0.058624 PRIMER_LEFT_4_SEQUENCE=aacaacggcaataaaaacgc PRIMER_RIGHT_4_SEQUENCE=agccggatcaatatcactcg PRIMER_LEFT_4=296,20 PRIMER_RIGHT_4=433,20 PRIMER_LEFT_4_TM=60.006 PRIMER_RIGHT_4_TM=60.059 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.9000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=138 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=4 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.013026 PRIMER_LEFT_0_PENALTY=0.006937 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_0=198,20 PRIMER_RIGHT_0=315,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.006 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=118 PRIMER_PAIR_1_PENALTY=0.032388 PRIMER_LEFT_1_PENALTY=0.032351 PRIMER_RIGHT_1_PENALTY=0.000037 PRIMER_LEFT_1_SEQUENCE=gacacacttgctgctttcca PRIMER_RIGHT_1_SEQUENCE=gtttcttgtacggtggcgat PRIMER_LEFT_1=609,20 PRIMER_RIGHT_1=868,20 PRIMER_LEFT_1_TM=60.032 PRIMER_RIGHT_1_TM=60.000 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=9.8000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=260 PRIMER_PAIR_2_PENALTY=0.037980 PRIMER_LEFT_2_PENALTY=0.031043 PRIMER_RIGHT_2_PENALTY=0.006937 PRIMER_LEFT_2_SEQUENCE=actggtctcccatcgtcaac PRIMER_RIGHT_2_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_2=106,20 PRIMER_RIGHT_2=217,20 PRIMER_LEFT_2_TM=59.969 PRIMER_RIGHT_2_TM=59.993 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=112 PRIMER_PAIR_3_PENALTY=0.052224 PRIMER_LEFT_3_PENALTY=0.000037 PRIMER_RIGHT_3_PENALTY=0.052187 PRIMER_LEFT_3_SEQUENCE=atcgccaccgtacaagaaac PRIMER_RIGHT_3_SEQUENCE=cggtgcacggtaactaaggt PRIMER_LEFT_3=849,20 PRIMER_RIGHT_3=1040,20 PRIMER_LEFT_3_TM=60.000 PRIMER_RIGHT_3_TM=60.052 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=192 PRIMER_PAIR_4_PENALTY=0.064713 PRIMER_LEFT_4_PENALTY=0.006089 PRIMER_RIGHT_4_PENALTY=0.058624 PRIMER_LEFT_4_SEQUENCE=aacaacggcaataaaaacgc PRIMER_RIGHT_4_SEQUENCE=agccggatcaatatcactcg PRIMER_LEFT_4=296,20 PRIMER_RIGHT_4=433,20 PRIMER_LEFT_4_TM=60.006 PRIMER_RIGHT_4_TM=60.059 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.9000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=138 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=3 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.013026 PRIMER_LEFT_0_PENALTY=0.006937 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_0=198,20 PRIMER_RIGHT_0=315,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.006 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=118 PRIMER_PAIR_1_PENALTY=0.032388 PRIMER_LEFT_1_PENALTY=0.032351 PRIMER_RIGHT_1_PENALTY=0.000037 PRIMER_LEFT_1_SEQUENCE=gacacacttgctgctttcca PRIMER_RIGHT_1_SEQUENCE=gtttcttgtacggtggcgat PRIMER_LEFT_1=609,20 PRIMER_RIGHT_1=868,20 PRIMER_LEFT_1_TM=60.032 PRIMER_RIGHT_1_TM=60.000 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=9.8000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=260 PRIMER_PAIR_2_PENALTY=0.037980 PRIMER_LEFT_2_PENALTY=0.031043 PRIMER_RIGHT_2_PENALTY=0.006937 PRIMER_LEFT_2_SEQUENCE=actggtctcccatcgtcaac PRIMER_RIGHT_2_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_2=106,20 PRIMER_RIGHT_2=217,20 PRIMER_LEFT_2_TM=59.969 PRIMER_RIGHT_2_TM=59.993 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=112 PRIMER_PAIR_3_PENALTY=0.052224 PRIMER_LEFT_3_PENALTY=0.000037 PRIMER_RIGHT_3_PENALTY=0.052187 PRIMER_LEFT_3_SEQUENCE=atcgccaccgtacaagaaac PRIMER_RIGHT_3_SEQUENCE=cggtgcacggtaactaaggt PRIMER_LEFT_3=849,20 PRIMER_RIGHT_3=1040,20 PRIMER_LEFT_3_TM=60.000 PRIMER_RIGHT_3_TM=60.052 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=192 PRIMER_PAIR_4_PENALTY=0.064713 PRIMER_LEFT_4_PENALTY=0.006089 PRIMER_RIGHT_4_PENALTY=0.058624 PRIMER_LEFT_4_SEQUENCE=aacaacggcaataaaaacgc PRIMER_RIGHT_4_SEQUENCE=agccggatcaatatcactcg PRIMER_LEFT_4=296,20 PRIMER_RIGHT_4=433,20 PRIMER_LEFT_4_TM=60.006 PRIMER_RIGHT_4_TM=60.059 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.9000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=138 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=2 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.037132 PRIMER_LEFT_0_PENALTY=0.031043 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_LEFT_0_SEQUENCE=actggtctcccatcgtcaac PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_0=106,20 PRIMER_RIGHT_0=315,20 PRIMER_LEFT_0_TM=59.969 PRIMER_RIGHT_0_TM=60.006 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=210 PRIMER_PAIR_1_PENALTY=0.052224 PRIMER_LEFT_1_PENALTY=0.000037 PRIMER_RIGHT_1_PENALTY=0.052187 PRIMER_LEFT_1_SEQUENCE=atcgccaccgtacaagaaac PRIMER_RIGHT_1_SEQUENCE=cggtgcacggtaactaaggt PRIMER_LEFT_1=849,20 PRIMER_RIGHT_1=1040,20 PRIMER_LEFT_1_TM=60.000 PRIMER_RIGHT_1_TM=60.052 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=192 PRIMER_PAIR_2_PENALTY=0.109692 PRIMER_LEFT_2_PENALTY=0.032351 PRIMER_RIGHT_2_PENALTY=0.077340 PRIMER_LEFT_2_SEQUENCE=gacacacttgctgctttcca PRIMER_RIGHT_2_SEQUENCE=aatgcggttatccgaagatg PRIMER_LEFT_2=609,20 PRIMER_RIGHT_2=820,20 PRIMER_LEFT_2_TM=60.032 PRIMER_RIGHT_2_TM=59.923 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=212 PRIMER_PAIR_3_PENALTY=0.154784 PRIMER_LEFT_3_PENALTY=0.051345 PRIMER_RIGHT_3_PENALTY=0.103439 PRIMER_LEFT_3_SEQUENCE=ttcattggaggctggttttc PRIMER_RIGHT_3_SEQUENCE=cctcttcaccacccgttcta PRIMER_LEFT_3=1184,20 PRIMER_RIGHT_3=1302,20 PRIMER_LEFT_3_TM=60.051 PRIMER_RIGHT_3_TM=60.103 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=6.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=119 PRIMER_PAIR_4_PENALTY=0.230339 PRIMER_LEFT_4_PENALTY=0.077340 PRIMER_RIGHT_4_PENALTY=0.152999 PRIMER_LEFT_4_SEQUENCE=catcttcggataaccgcatt PRIMER_RIGHT_4_SEQUENCE=tcagcatgcagttgaaaacc PRIMER_LEFT_4=801,20 PRIMER_RIGHT_4=1011,20 PRIMER_LEFT_4_TM=59.923 PRIMER_RIGHT_4_TM=59.847 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=211 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=1 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.154784 PRIMER_LEFT_0_PENALTY=0.051345 PRIMER_RIGHT_0_PENALTY=0.103439 PRIMER_LEFT_0_SEQUENCE=ttcattggaggctggttttc PRIMER_RIGHT_0_SEQUENCE=cctcttcaccacccgttcta PRIMER_LEFT_0=1184,20 PRIMER_RIGHT_0=1302,20 PRIMER_LEFT_0_TM=60.051 PRIMER_RIGHT_0_TM=60.103 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=119 PRIMER_PAIR_1_PENALTY=0.373044 PRIMER_LEFT_1_PENALTY=0.277599 PRIMER_RIGHT_1_PENALTY=0.095445 PRIMER_LEFT_1_SEQUENCE=gggcctcattaatccaatca PRIMER_RIGHT_1_SEQUENCE=aagcggcaaaggaggttaat PRIMER_LEFT_1=757,20 PRIMER_RIGHT_1=897,20 PRIMER_LEFT_1_TM=59.722 PRIMER_RIGHT_1_TM=60.095 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.2000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=141 PRIMER_PAIR_2_PENALTY=0.436761 PRIMER_LEFT_2_PENALTY=0.385416 PRIMER_RIGHT_2_PENALTY=0.051345 PRIMER_LEFT_2_SEQUENCE=acggagcaaaaacgttgaat PRIMER_RIGHT_2_SEQUENCE=gaaaaccagcctccaatgaa PRIMER_LEFT_2=1063,20 PRIMER_RIGHT_2=1203,20 PRIMER_LEFT_2_TM=59.615 PRIMER_RIGHT_2_TM=60.051 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=141 PRIMER_PAIR_3_PENALTY=0.605909 PRIMER_LEFT_3_PENALTY=0.420643 PRIMER_RIGHT_3_PENALTY=0.185267 PRIMER_LEFT_3_SEQUENCE=agagacttgccgctcatttc PRIMER_RIGHT_3_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_3=475,20 PRIMER_RIGHT_3=697,20 PRIMER_LEFT_3_TM=59.579 PRIMER_RIGHT_3_TM=60.185 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=223 PRIMER_PAIR_4_PENALTY=0.821843 PRIMER_LEFT_4_PENALTY=0.815754 PRIMER_RIGHT_4_PENALTY=0.006089 PRIMER_LEFT_4_SEQUENCE=cacctcatggacaccatcat PRIMER_RIGHT_4_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_4=162,20 PRIMER_RIGHT_4=315,20 PRIMER_LEFT_4_TM=59.184 PRIMER_RIGHT_4_TM=60.006 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.5000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=154 = SEQUENCE_ID=Pick primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_END_GC=0 PRIMER_MIN_THREE_PRIME_DISTANCE=2 PRIMER_NUM_RETURN=5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.353221 PRIMER_LEFT_0_PENALTY=1.293240 PRIMER_RIGHT_0_PENALTY=1.059980 PRIMER_LEFT_0_SEQUENCE=tttccgtccttctgcattaaa PRIMER_RIGHT_0_SEQUENCE=cagcctccaatgaatcaaaaa PRIMER_LEFT_0=950,21 PRIMER_RIGHT_0=1197,21 PRIMER_LEFT_0_TM=59.707 PRIMER_RIGHT_0_TM=60.060 PRIMER_LEFT_0_GC_PERCENT=38.095 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=248 PRIMER_PAIR_1_PENALTY=2.376287 PRIMER_LEFT_1_PENALTY=1.301938 PRIMER_RIGHT_1_PENALTY=1.074349 PRIMER_LEFT_1_SEQUENCE=tccctcggacaaccatttt PRIMER_RIGHT_1_SEQUENCE=tttccacaaacccaccaataa PRIMER_LEFT_1=897,19 PRIMER_RIGHT_1=1143,21 PRIMER_LEFT_1_TM=60.302 PRIMER_RIGHT_1_TM=60.074 PRIMER_LEFT_1_GC_PERCENT=47.368 PRIMER_RIGHT_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=6.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=247 PRIMER_PAIR_2_PENALTY=2.558875 PRIMER_LEFT_2_PENALTY=0.543622 PRIMER_RIGHT_2_PENALTY=2.015253 PRIMER_LEFT_2_SEQUENCE=tgatccggctcaaggaatta PRIMER_RIGHT_2_SEQUENCE=tgttgtggtgatggtggttatt PRIMER_LEFT_2=424,20 PRIMER_RIGHT_2=561,22 PRIMER_LEFT_2_TM=60.544 PRIMER_RIGHT_2_TM=60.015 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.2000 PRIMER_RIGHT_2_END_STABILITY=6.2000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=138 PRIMER_PAIR_3_PENALTY=3.451501 PRIMER_LEFT_3_PENALTY=1.482208 PRIMER_RIGHT_3_PENALTY=1.969292 PRIMER_LEFT_3_SEQUENCE=caacaacaacaacggcaataa PRIMER_RIGHT_3_SEQUENCE=tcgagctaagtcacggttttt PRIMER_LEFT_3=289,21 PRIMER_RIGHT_3=416,21 PRIMER_LEFT_3_TM=59.518 PRIMER_RIGHT_3_TM=59.031 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.2000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=128 PRIMER_PAIR_4_PENALTY=3.999815 PRIMER_LEFT_4_PENALTY=2.675181 PRIMER_RIGHT_4_PENALTY=1.324634 PRIMER_LEFT_4_SEQUENCE=ccctacttccgacgaggattat PRIMER_RIGHT_4_SEQUENCE=caccaacttcatgcgttttt PRIMER_LEFT_4=68,22 PRIMER_RIGHT_4=327,20 PRIMER_LEFT_4_TM=60.675 PRIMER_RIGHT_4_TM=58.675 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=260 = primer3-2.3.6/test/long_seq_input0000664000077300007730000035205212231156245016015 0ustar ioanaioanaSEQUENCE_EXCLUDED_REGION=0, 37 SEQUENCE_EXCLUDED_REGION=447, 285 SEQUENCE_EXCLUDED_REGION=961, 290 SEQUENCE_EXCLUDED_REGION=2016, 262 SEQUENCE_EXCLUDED_REGION=2564, 287 SEQUENCE_EXCLUDED_REGION=2880, 99 SEQUENCE_EXCLUDED_REGION=3489, 263 SEQUENCE_EXCLUDED_REGION=3820, 283 SEQUENCE_EXCLUDED_REGION=4495, 277 SEQUENCE_EXCLUDED_REGION=4807, 286 SEQUENCE_EXCLUDED_REGION=5100, 276 SEQUENCE_EXCLUDED_REGION=6209, 288 SEQUENCE_EXCLUDED_REGION=6828, 89 SEQUENCE_EXCLUDED_REGION=6981, 129 SEQUENCE_EXCLUDED_REGION=7127, 205 SEQUENCE_EXCLUDED_REGION=7388, 132 SEQUENCE_EXCLUDED_REGION=8552, 166 SEQUENCE_EXCLUDED_REGION=8799, 277 SEQUENCE_EXCLUDED_REGION=9101, 196 SEQUENCE_EXCLUDED_REGION=9300, 301 SEQUENCE_EXCLUDED_REGION=10228, 283 SEQUENCE_EXCLUDED_REGION=10530, 281 SEQUENCE_EXCLUDED_REGION=11013, 243 SEQUENCE_EXCLUDED_REGION=11304, 281 SEQUENCE_EXCLUDED_REGION=11626, 255 SEQUENCE_EXCLUDED_REGION=11938, 291 SEQUENCE_EXCLUDED_REGION=12254, 284 SEQUENCE_EXCLUDED_REGION=12557, 147 SEQUENCE_EXCLUDED_REGION=13103, 160 SEQUENCE_EXCLUDED_REGION=13266, 288 SEQUENCE_EXCLUDED_REGION=14765, 732 SEQUENCE_EXCLUDED_REGION=15794, 127 SEQUENCE_EXCLUDED_REGION=15997, 85 SEQUENCE_EXCLUDED_REGION=16402, 51 SEQUENCE_EXCLUDED_REGION=16880, 59 SEQUENCE_EXCLUDED_REGION=17065, 124 SEQUENCE_EXCLUDED_REGION=17708, 65 SEQUENCE_EXCLUDED_REGION=17929, 78 SEQUENCE_EXCLUDED_REGION=18125, 124 SEQUENCE_EXCLUDED_REGION=18356, 65 SEQUENCE_EXCLUDED_REGION=18581, 87 SEQUENCE_EXCLUDED_REGION=18692, 183 SEQUENCE_EXCLUDED_REGION=19248, 275 SEQUENCE_EXCLUDED_REGION=20438, 24 SEQUENCE_EXCLUDED_REGION=20722, 99 SEQUENCE_EXCLUDED_REGION=20870, 151 SEQUENCE_EXCLUDED_REGION=21401, 114 SEQUENCE_EXCLUDED_REGION=21928, 284 SEQUENCE_EXCLUDED_REGION=22834, 155 SEQUENCE_EXCLUDED_REGION=22992, 287 SEQUENCE_EXCLUDED_REGION=23282, 138 SEQUENCE_EXCLUDED_REGION=23435, 69 SEQUENCE_EXCLUDED_REGION=23459, 280 SEQUENCE_EXCLUDED_REGION=23753, 288 SEQUENCE_EXCLUDED_REGION=24338, 290 SEQUENCE_EXCLUDED_REGION=24591, 511 SEQUENCE_EXCLUDED_REGION=26124, 287 SEQUENCE_EXCLUDED_REGION=27286, 276 SEQUENCE_EXCLUDED_REGION=27667, 278 SEQUENCE_EXCLUDED_REGION=28313, 91 SEQUENCE_EXCLUDED_REGION=28585, 286 SEQUENCE_EXCLUDED_REGION=28957, 286 SEQUENCE_EXCLUDED_REGION=29255, 287 SEQUENCE_EXCLUDED_REGION=29573, 45 SEQUENCE_EXCLUDED_REGION=29801, 468 SEQUENCE_EXCLUDED_REGION=30461, 180 SEQUENCE_EXCLUDED_REGION=30701, 173 SEQUENCE_EXCLUDED_REGION=31589, 220 SEQUENCE_EXCLUDED_REGION=31992, 287 SEQUENCE_EXCLUDED_REGION=32545, 277 SEQUENCE_EXCLUDED_REGION=32848, 68 SEQUENCE_EXCLUDED_REGION=32956, 89 SEQUENCE_EXCLUDED_REGION=33079, 279 SEQUENCE_EXCLUDED_REGION=33371, 117 SEQUENCE_EXCLUDED_REGION=33758, 284 SEQUENCE_EXCLUDED_REGION=34333, 282 SEQUENCE_EXCLUDED_REGION=35341, 108 SEQUENCE_EXCLUDED_REGION=36347, 247 SEQUENCE_EXCLUDED_REGION=36687, 275 SEQUENCE_EXCLUDED_REGION=37003, 286 SEQUENCE_EXCLUDED_REGION=37918, 217 SEQUENCE_EXCLUDED_REGION=38979, 286 SEQUENCE_EXCLUDED_REGION=39515, 700 SEQUENCE_EXCLUDED_REGION=40312, 93 SEQUENCE_EXCLUDED_REGION=40510, 77 SEQUENCE_EXCLUDED_REGION=40691, 236 SEQUENCE_EXCLUDED_REGION=41066, 187 SEQUENCE_EXCLUDED_REGION=41700, 94 SEQUENCE_EXCLUDED_REGION=42159, 126 SEQUENCE_EXCLUDED_REGION=42462, 159 SEQUENCE_EXCLUDED_REGION=43291, 218 SEQUENCE_EXCLUDED_REGION=44058, 109 SEQUENCE_EXCLUDED_REGION=44262, 38 SEQUENCE_EXCLUDED_REGION=44682, 275 SEQUENCE_EXCLUDED_REGION=45145, 285 SEQUENCE_EXCLUDED_REGION=45464, 277 SEQUENCE_EXCLUDED_REGION=46126, 276 SEQUENCE_EXCLUDED_REGION=46471, 288 SEQUENCE_EXCLUDED_REGION=46767, 118 SEQUENCE_EXCLUDED_REGION=46888, 282 SEQUENCE_EXCLUDED_REGION=47260, 94 SEQUENCE_EXCLUDED_REGION=47372, 94 SEQUENCE_EXCLUDED_REGION=47495, 134 SEQUENCE_EXCLUDED_REGION=47854, 285 SEQUENCE_EXCLUDED_REGION=48185, 122 SEQUENCE_EXCLUDED_REGION=48310, 284 SEQUENCE_EXCLUDED_REGION=48598, 142 SEQUENCE_EXCLUDED_REGION=49033, 286 SEQUENCE_EXCLUDED_REGION=49926, 273 SEQUENCE_EXCLUDED_REGION=50195, 72 SEQUENCE_EXCLUDED_REGION=50218, 286 SEQUENCE_EXCLUDED_REGION=50994, 262 SEQUENCE_EXCLUDED_REGION=51822, 171 SEQUENCE_EXCLUDED_REGION=51999, 280 SEQUENCE_EXCLUDED_REGION=52294, 284 SEQUENCE_EXCLUDED_REGION=52581, 132 SEQUENCE_EXCLUDED_REGION=53675, 287 SEQUENCE_EXCLUDED_REGION=54157, 128 SEQUENCE_EXCLUDED_REGION=54796, 1260 SEQUENCE_EXCLUDED_REGION=54910, 466 SEQUENCE_EXCLUDED_REGION=56696, 288 SEQUENCE_EXCLUDED_REGION=57124, 275 SEQUENCE_EXCLUDED_REGION=57404, 166 SEQUENCE_EXCLUDED_REGION=0,58 SEQUENCE_EXCLUDED_REGION=5975,51652 PRIMER_OPT_TM=65 PRIMER_MIN_TM=60 PRIMER_MAX_TM=70 SEQUENCE_TEMPLATE=CAAAGTTCTGGGATTACAGGCGTGAGCCACTGCGCCCGGCGTAGGCCAGTTTCTATACTGTGGAACATTAAGTGGACTTGTCTGCTTTTCCAAACTTTGGCGCCACTAGCAAGGTGCAGAGGCTTTGTGGTAAAGGAAGAGAAAGAAGGCAGAAAGTTGGGGAAAAATATGATGGGAATGTGTAGAAAGAAGGTCGGAAGGCAGTACAGAAGACAGTACAGAGTTGGGGGGGGGGGATCATATGTTCCCAAAGCTTATTGGTGCAACTGGAATCAATCTGCACACTTCAACCTCTTGTCAAGACTGTGATGGAGTCGGAGGTATTTGCCCCGGGTAGGTGCACAAGACGCATGGTTGCAGATGTGGAAACAGAACAGAACAAAACCACCCACGTCTCTAATGTTTCAAATGAATGATATACATTTGTTTTTATTACTTATTTATTTATTTTTGAGACAGGGTCTCGCTCTGTCGCCTAGGCTGGAGTGCAGTGGCACAAGCTCGGCTGACTGCAACCTCCGCCTCCCGGATTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGTGCGCCACTACCACCCGGCTAATTTTTATATTTTTTAGTAGAGACAGGGTTTGCCATGTTGGCCAGGCTGGTCTCAAATTCGTGACCTCAAACGATCCACCCGCCTCAGCCTCCCTAAGTGCTGGGATTACAGGTGTGAGCCACCGTGCCCAGCCAAATAATATACTTTTAATAAATTTATTGACATTTAAATTTATTTTAAAACTTTATTTATTTATTTAGAGACAGGGTCTCGCTCTGTTACCCAGGCTGGAATGCAGTAGTGCCATCATAATCACTGCAGCCTCGAACCCCTGGGCTCAAGCGATCTTCCCACCTCAGTATCCCAAGTAGCTGGGACTACAGGAGCATGCCACCATGCCCAGCTTAAAAAGTTTTTTTGGCCGGGTGCGGTGGCTAATGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACACGGTGAGACCCCGTCTCTACTAAAAATACAAACAAATTAGCCGGGCGTGGTGGTGGGCGCCTGTAGTCCCAGCTATTTGGGAGGCTGAGGCAGGACAATCTCTTGAACCCCGGGTGGGGGGAGGTTGCAGTGAGCCGAGATCGCGCCACTCTCCTCCAGCCTGGGCAACAGAGTGAGACTCCCATCTCAAAAAAAAAAAAATTGATTTTTGTGTGTGTTTTACAAAGTTCAAGTTAGTATGTGCGTATAATATGAGCAAATATACATATGCTGAGGGTTGTTACAAGGGTTTGATGTGTGGATGATCAAAAACATTTGCAGCCATAGCTCTCAGCCATTGTTTAGCCTCCAGGGATCACTAAGGCAGAAGCGATTTGTGTGTTTGTACTTGGTGTTCTGGAAAGGTTGTTCTGTCCCTTTCAAATGGGGTTGAAGGGTCCTGATGGAAGTAGACAGTATTTAGACTAGGACAGGTGAAAGGGCTGGACTAGGCACATGCTGGGGCTGAAGATTGCAATTTGGAAAACGGCAGTGAGGGTAGGGATTGTCTGAGGATGGGGTAAAATTGGCCAGAGGGAGAAAGGAGTCAGCTGGAAAAGCCAAAGAAGGAAAACCCAAAAAGTTGTGTCCTTCTTGAAGGAAAAGGGAATTTTTGTATTCCACTTGCCTGTGCTGTTGGGCCTGCCTGACACTGTCAGTAAAATTCACCTGGAACTAGCCACTTGGCAGCCACCAATTCTTGTTTTTGCCAGGTGTCAAGGAGAGCAAATGCAGTCAGAAGACTGCTGTCTGTTATTGTCAATCACTTCAGCCTTCCCTGAAAAGCCCAAGTGGGCAGGTGGGATATCTGACATGAAGAATTTGGTGGCTTTGTCTGCAGGAAAAGGGAATAGCAAAATTAAAGGCAAGACAATGTTCCTAGTTGGTTTAGGGAATCAGTAATTTACCCCAGGGTGCGGGTGGCTCACGCCTGTTATCTCAGCACTTTGGGAGACCGAGGCGGGTGGATCACTTGAGCTCAGGAGTTCAAGACCAGCCTGGGCAACATGGCAAAACCCCATCTCTACAAAAAATACAAAAATTAGCCTGGCGTGGTGGCATGCGCCTGTACTCCCAGCTACTCGGAAGGCTGACGTGGGAGGGTCGCTTGAACCCCGGGAGATGGAGGCTGCAGTGAGCTGAGATGGCACCACTGCACTCCAGCCTGGGCAACAAAGTGAGACCTTGACTCAAAATAATAATAATAATAATAGTAATAATAATAATAATTTACCCTAGAAGAGAAGGAGGAAGAGGTGAGGATATCTAGTGCCCAAAGAGAAGTCATCTGGACCGAACAAACAGATGGAACAAATGATAGCAACAGCTGAAACAACATCAACACATTTCAGATGGGAGGCCTGCCAGGTATTATGTAATAAGGTCACAGTGGTGACCCTGAGAGGCATGCCTACCTCTGCTCTTGAGGCAGGGGCCTCTAGAAACAGGATTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTCACTCTATCGCCCGGGCTGGAGTGCAGTGGCACAGTCTTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGTGATTCTCCTACCTCAGCCTCCCAAGTAGCTGGGATTACAGGTATGTACCACCACACCCAGCTAAGTTTTGTATTTTTAGTAGAGGTGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGACCTCCCAAAGTGCTGGGATTACAGGCATAAGCCACCGTGCCTGGCCCAGAAACAGTATTTGATAAATAAAGAGGCCAGGTGAGGTGGCTCATACCTGTAATCCCAGCATTTTGGGAGGCTGAGGAGGGAAGATTGCTTGAGCCCAGGAGTTTGAGACCAGCCCGGGCATCATGGGAAGACCCTATTTCTACATAAACAGATTTTTAAAAAAATTTAAAAAGGCATGGAAGGCATATGCTAGTGTAAGTATGAGGTACATTGAGTATCCACAGGGAAGTGTAATGACACCTGTCACTTTTGCCTGCCCAGCATCAGGTTGCAGCACCTTAGTTTTCTCTGGGGGAACCATCCCTCCTCCACTCTCAGTACACATGGTTTGCATGTGGATGACCCCAACGCTGGGCCCTGGAGTGGGCACATAACCAGGTATAACCAGCCAGCCACTCAGAGTGGGCAGACATCATGCCATAATCCAGTCTGGACCAGTGAGGGTTAGTCCCCAGGAACTTGTACTGAAGTATTGGGAATGCCATCCTAAGTCACAGAGAGGGAGACTAAAAGAAAATGGTATTTATTTGGAATGGGTACTGCAATGGGAATGTGTGTGCCATAGTAAACTGTGTATATTCAGGGAGGCGAAGGGAGAAAAAGGGTTTTGTTGTTGTTGTTTTTTAAGGTAGGGTCTCACTCTGTTGCCCAGGGTGGAGTGCAGTGGTGTGATACTGGCTAACTGCAGCCTTCACCTCCTGGACTCGGGTGACCCTTCCACTTCAGCCCCCTCGAGTAGCTGGGACTACAGGCATGTGCCACCATGCCTGGCTAATTTTTTTGCATTTTTTGTAGAGATGGGGTTTAACCATGTTGCCTTACACTGGTCCCAAACTCCTGGGCTTAAGCAATTCTCCTGTCTCGGCCTTCCAAAGTGCTGGGATTACAAACATGAGCCATTGCAGCAGGCTGACAAAGGTGTTTTTTGTTTGTTTGTTTGTTTGTTTGTTTGTCTGTTTGTTTGTTTTTGAGATGGAGTTTGGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGCGATCTCAGCTCACTGCACCCTCCGCCTCCTGGATTCAGGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACGGGCACCCGCCACCACGCCCGGCTGATTTTGTATTTTTAGTAGAGACGGGGTTTCACTATGTTGGTCAGACTGGTCTTGAACTCCCGACCTCAGGTGATCCGCCTTCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAACCACTGTGCCCGGCTGAGAAAAGTTTTTAAAGAAAAAATGAGAATTACATAATTGTTTTCAGGTAATTATTCTTGGCTACAAGGATTAATAACAAAGGTGGCATCAGTCCAAATTTGGACAGGCAGCTGCTGGGTAGATGTGTTCACAGAAGTATTTTCTGTGTGTAAGGTGATGAGACTGCAAAAACTATATCACTCTGGTGATGATAGCCGTTGTACAGGGTTATAGTTTTTGCAGTCTCATGATGGGTTTTGCTATCAGGCATCACACATGAGAACCTTCCCTTCATGGCCTTCCCCAGCTCCGGTTTTCGGGTTTTTTTTTTTTTTTTAAGGTGGGGTCTTGCTATGTGACCCTGGCTGGAAGTGCAGTGGCTTTTCACGGAGCGATCCTACTACTGATCAGCATGGGAGTTTTTATTTGCTCTGTTTCTGAGCTGGGCTGGTTCACTCTTCCTTAGGAAACCTGGTGGTCTCCTGCTCCCAGGAGATTACCATATTGATGCTGAGTTTAGTGGAGACACCAGATCAGAATAGCATACCACAGCCCAGAAATCCTGGGCTCAAGTGGTCCTCCTGCCTCAGCCTCCCCAGTAGCTAGGAATACAGGTGTGTACTACCATGCCTGGCTAATTTTTAAAAATTTTTCGTAGAGACAGGGTCTCCCTATGTTGCTCAGGCTCATCTCGAACTCCTAGGCTCAAGGGATCCTCCCATGACAGCCTCCCAAAGTGCTAGACTACAGGGGTGAGCCACCGCACCTGGCCTAGCATGGAAGTTTTAACCTGCTCCATTTCCAGCCTGGGCTGGTTCATTCCTCCCTAGACAACCTAGTGGTCCTCCATTCCCAGGATACTGATGCTGAGCTTTTTGTGGACACCTGATCGGCCTGGCATACTACAGCCCAGAACTCCTGGGCTCAAACAATTCTCCTGCTGCAGCCTCCTGAGTAGCTAGGACTACAGGCATGTGCCACCACACCTGGCAGGGTTTGTTTGTTTTTTTTTTTTTTTAATGAGACAGAGTCTCACTTTGTCACCCAGGCTGGAGTGCAGTGGTGCGATCTTGGCTCACTGCAACCTCCGTCTCCCAGGTTCCAGCAATTCTCCCACCTCAACCTCCTGAGTAGCTGGGACTACAGGCCTGCATCACCATGCCTGGCTAATTTTTGTATTTTTACTAGAGACAGGGTTTTACCATGTTGGCCAGGATGGTCTCAAACTCCTGACCTCAAGTGATCCACCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCACCTGTTTTGTTTTTAATACAGGTGACTCCATTTTGCTTCTGGCAACTTTCACATTTCCCCTTTTTGATCGTGATCTTTCTTCAAAAGCAGTAGGTTTGGATTGTCCCTCAGTGCTGGGATGGACCTGTCCTGGGTTGTTGGTCTGGTTCCAGATAAGAGGGAGTGATTGACAACTAAGAGTCAGTGTCAAAACCCCTTTAGGCACATCTGAGCAACAGTGGAAATTTGGAGAGAGTTGCACTCAGACTAAGTCTACCTGAAGTCCATCGTTAAATTCTATTTTTTCTGTTCTGTGGTCTTTTGGCCATCATCTGGAAGCCCCGTGCCAGCATACTCTGTTAGCAGCTGTACTTCTGCAGAAGTTTCACAAGTAACAAGTACAAATTGTTAAAAGGAAAATACAAATTAATATTAGTAGTAATATGATACCCCCAGTTTTCATAATAGTTTTGAGCTATGAACCTAGGCTTAAAGGCAATCAAGTGAATAAATCAAATGACCATGGGGAATTAGGTGAGACCCATTATAACCTATGTGACCTGTTATAACCTATGTTTTGTAATTTCGTGTATATGGGGCCTCAAGTTCCCCAGGGGAATTTATCCAGGTTCAGCATGTGCTATTAGCAATAGAACAGATATTTCCTGATTTAACCAATAGATGCTGAAGGATCTCTTAGGTCAGGTTCTGTGAGGTTACTGACAGAAGCTATTGATTGTGCAATTTCAATTACACCATTATCCTGTCAACGAAAAGGCAGACATAAGAAAGGAAAAATTAAGAGTGACAAGATTTTCATTTTGATGGGTGAAGCTCTCAACTTCTTTTTTTTTTGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGCAGCATGATCTTGGCTTACTGCAACCTCCAACTCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGCGTGCCACCACAGCCAGCTAATTTTTAAAAAATATTTTTGGTAGAGATGGGTTTCACCATGTTGGCCAGACTGGTCTTGAACTCCTGACCCCCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATGACAGGCCTGAAGCCACCGCGCCCAGCTGAAGCGCTCAACTTCTTGTTCTGGTTTGCAGTTTGAGTGTCTCTGGTTATGGCATCAGGTGGTTTGGAGAACTTCCTGTGTGACCCATACATCAGGCATGAGACTTGTCCCTGGATATTTATACCAAGTTTTCCAGCTTCAGCTTCAAGGCCTTTAGCAACATAACAGGTTTTTTTCTTAGTTGGAGAGTTTTAGCCAAATATTAGAGGAAATTAGGAGGATTTGGGGTCTAGTCCCGTCTTCATGTAGATAAGAAACAATGCAAAGGGCTGCAATCTAATAGCAGGCATATTTTAGTGTTTTTCCTTTAGAAACAACTTTTTCTGGGAGGCCAAGACGGGCGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAAAAAAAAAAAAAAAAAAATAGCCAGGCGTGGTGGCACGCACCTGTAGTCCCAGCTACAGATTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCAATCTCAGCTCACTGCAACCCCCACCTCCCAGGTTGAAGCAACACTCATGCTTCCACCTCCCAAGTAGCTGGGATTACAAGCATGAGCCACTGTTTTTGAGACAGGGTCTCACTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCCATCATGGCTCACTACAGTCTCAACCTCCTGGGCTCAAGCAATCCTCTCCTCCTACCTGAGCTCCCTGAGTAGCTGGGCATACAGGTGCGCACTACCACACTGGCTATTTTTTTTTTTTTTGTGGTAGAGATGGGGGTCTCCTTATGTTGCCCAGGCTAGTTTTTTTTTTTTTTTTTTTAACTTGGGTACCTTTGGTATTTTTAGTAGAGACTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGGCAGGCTGGTCTCAAACTCCTGACCTCGTGATCCGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAAGCACCGCGCCCCGCCTGCCCAGGCTAGTTTTAGAACTCCTGGGCTTAAGTCATCACCTGTCTCAGCCTTCTAAAGTGCTGAGATTACAGGCGTGACTCACCATGCCCACCTAATTACTATATTTTTGATAAAATAATTAATGCTTTTAATTTTTCCCTTTAATTAATTAGATATTTTTCATATATTTTGGTAGAAAATATTACACAGAATGTGAAAACGTACGGACACATGATTACATAGAAACAGATTCCCTAGCTTTCATTTTGAAATTTTTGTCATGAGACAGTACAATATAGTAATATACGCTTGCCAGTTTATAAAAGGACAGTTGGATCCAATTTCTGACAAAATGAGACCCGTTCGTGTGGCTGAGCATTATTTGACCCCATAGGTAATCTTATGAAGGCTGAAGATCAAAATTTTGGGTAAAGCAGCGTCTACAGCAGTTTGATTTAAAATATCTTTTTAAAATCCTTTTATTCCATATCAAATGAGTTTAGAGGTTAAATATTCAAATGTTCACATTTCAGTTAGGACTAGCTGAATTGTATGAGAAAAACAGAATTTCCAGTGGCCAATCTGGTTTGCTTGATTAGTCAGCACAGGTGGAGAGGCACTTTTCAAAAAGATATTTACAGTTGTTTTTTTTCCTCAGCTTTTTCTGGATTGTACATGACAGACAAAGCCAAATTTTTATGCTGGACAGAGATACCTTATGTGATTGCTGTGTGCTCAAGAGTTTGACCTGTTTGATCTGAGAACCTAACTTTCACAAACATTGATCTAGTTCTTTCCTTTTTAGACTATCAATCTTTTAATTAACTGTTCCGTCACCCTAAGCAATTGTCAGCTAGGCAAACCTCCATACATGTTTCTGAAAGGAATGACTCCTAAGTGTACAAGGCTAGGTTTTTGGTTGCCATGGAGCTGTTGTAATTTGAAAGCACCCTTTTTTCTTTTCTTTTTCTTGGCTGAAATGCCCTAAGGAAAAGTTATAAACAGCTTTTGAAATCACCAATGTTAGATTATCTTTTTTTATTCTTATTTTTTTGAGACAAGATCTCACTCCATCGCCCAGGCTAGAGTGCAGTAGTGTGTCACGGCTCAACGCAGCCTTGAACTTCCAGGCTCAAGTGATCCTCCCACCTCAGCCTCCTGAATAGCTGGGACCACAGGTGTGTGCCTCCACGCTAGCTACCTTTTTGTATTTTTAATGTAGGGTTGGGGTCTGATCAGGTTGCCCAGGGTGAAATTATCTTAATACAATTATCTTAATACAAGTGAAGAAGCTGGCTGGGCACGGTGGCCACGCCTGTAATTCTAGCACTTTGAGAGGCCAAGATGAGTGGATCATGTGAGCTCAGGAGATAGAGACCAGCCTGAACAACATGATGAAACCCCATCTCTACAAAAATTAGCCAGGCGTGGTGGCAGGTGCCTGTGTTCCCAACTCCTTGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGGTTGAGGCTGCAGTGAGCTGAGACTGCACCACTGCACTCCAGCCTGGGTGGCAGAGTGAGACCCTGTCTCAAAAAAAAAAAAAAAAAAAAAGAAGAAAAAGGCCAGGTGCGGTGGTTCACACCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCAGATCATGAGGTCAGGAGATCGAGACCATCCTGGCCAACATGATAAAATACAAAAAATTAGCCAGGCATGGTGGCGCATGCCTGTAGTCCCAGCTACTCGGGACGCTAAGGCAGTGGAATTCCTTGAACCCGGGAGGCAGAGGTTGCCGAGATCGTGCCAGCCTGGTGACAGAGCAAGACTCCGCCTCAAAAAAAAAAAAAAAGAAGAAGAAGAAGAAAAAGCCAACAGAGTCAGCAGAGAGGAGGAAGAAAAGAAAAGCAGATAGAGAAGTTAGGCGCCTCTACATACCAGTGTTTTAGTTTTATTTATTTAATTTTATTTTTTATTTTAATTTTATTTTTTTGAAACAGGGTCTCACTCTGTTTCCCAGGCTGGCCTTGAATTCCTGGGCTCAAATAATCCTCCTGTCTTGGCCTCCAAAAGTGCTGGGATAACAGGCATGAGCCACCGTGCCCAGCCCCAATTCCTTTTAAAGGTGATTTTGTTTCGGGTCTCACTTATTTTTTTTTTCTTTTCTTTTCTTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTCTGAGATAGCCTTGACCCCTGGGCTCAAGCCATCCTCCCACCTCAGCCTCCCTAGTAGCTGGGACTACAGGTGTGCACCACCACACCTGGCTAATTAATTTTTTTTTTTTTTTTTTAGAGACAGGGTCTTGCTGTGTTGCCCAGGATGGTGTCAAATTCCTGGGCTCAAGTGATCCTCCTGCCTTGGACTCTCAAGTGCTGGGATGACAGGTGTGAGCCACCACGCCCAGCTGTAGGTCCCACTTCTGACTCTAGTTATGTCATCCTAAATAACAGGCTCTCTGAGAGAAAATGATATTTATTCTGGAATGCGTTGCAGTGGGAATGCATGTGCCATGGTAAACTGTATGGTAAACTGTGTGCATATACAGGGAGGCAAAAGAAGGCAAAGGTTTTTCAAGGAAAAGTGAAGAGGATCACCTAATTGTTTTGAGAGAATTCTCCTTGGCTACAAGGATCGATAACAAGGGTGACCTCAGTATGAGGTTGGACAGGGTTAGGGTTAAGTTGCTCAGCTGGGTGCGGTGGCTCACGCCTATAATCCTAGCACTTTGGGAGGCTGAGGCGGGTGGATCACCTGAGGTCAGGAGTTCAAGAGCAGCCTGGCCAACAAGGTAAAACCCTGTCTCTACTAAAAACACAAAAATGAGCCAGGCGTGGTAGCGCATGCCTGTGATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTAGGAGGCGGAGGTTGTGGTGAGCCAAGATTGTGCCACTATACTCCAGCCTGGGTGACTGATCCAGACTCCGTTTCAGAAAAAAAAAAAAAAAAAAGTCAGCCGGGCACGGTAGCTCACGCCTTTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACTTGAAGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCGTGTCTCTACTAAAAATACAAAAATTAGCTGGGTGTGGTGGTGCACACCTGCAATCCCAGCTACTTGGGAGGCTGAGGCAGAATTGCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCCGAGATTGCGCCACTGCGCTTCAGCCTGGGTAACAAGAGCGAAACTCCGTCTCAAAAAGTTGCTGGGCAGATGTCCTTGCAGTATTTTTTTTCTGTGGAAAGCTGTGGTTTCTGCATTTGCAGCCTTCCCCAGCTCTGTTTTTTGTTTTTTAACACAAGTGACTCCATTTTGATTCTGACAACTGTCATGGCAAGGAAGGCTGCCATGTTGGAAAATGAAGCCAATACAGAGAGAAAAACAAAGCCAAGAGGTGGAGAGAGCCCAAGTCCCTTGGGCTTCGGGGTCCATTACAGGAACAATGCAATTCTGCCATCCTCCCTTTTTTTTTTTTTTTTTTTTTGCTTATGCCAGTTTGAGTGTTGAATTCTGGCATTGTAAGTGAGGACATCCTAACTCCTAAAGGGCAGGCCCAGGGGGCGATACCCCTGGCAAAACCAGGACCGTGGGATATGTCAAGCTTACCTCCTCTTTGGGAAGTCAGGCAGGCTGTGCTCCATGCCATCAGCTTCCTAGCCTGTGTTGGGCTGTGACACTGGTGACCCCAGTGAGGGTGCAGCCCCATGAAGAGGTAGAGGCTGTCACTGTGGCTCACTACGGTGTTCCTGGACCAGTTCAGGCACTTGGTGAATGTTTAGTGAACAAATGAGCTGAAGGAACAAATGGAGGAAGGCTGGGCAATCACAGCAGTTGCTGGAGAAGTGTCGGGGAGCCCAAGCCCCTCCCAGCCTGGCAGTATAGACACTCCGTGAGTTTCCGTTTCATTTAGGGGCAAGCTAGTAACCTCGTGGAATTGTACTTATCTGGCTTGTAGTCTTGCTACACCGGGAACTCTCCTATCCCCACTAGGGACCTTTACTTTTTTGTACAGTGGGGACAATGGCCACACTTACTGGCTAGGGTTGTGGTGAGGTGTTACCTATGGTATAACTGAGGTATTGATGCAAGAAGGAAGTTGAGATTTGTATCAACATATGGGACCATGTGGGTACAGATGAGACAGCACTGGTCACATGTCGATCACTAGAGGGATGAGCACATCAGAGTTGATGACAGTCATCTGTTGGTATCCGATAGGTATTTGTTCCAGGGCACCCTATAGCTGCCAAAGTCCAGGGATGCTCAAGTCCCTGATATAAAATGGATACATTATAGTTATACATATTTATTCCCATAGATTTTTTTTTCTTTTGAGATGGAGTCTCGCTCTGTCACCCAGGCTGGAGGGCAGTGGTGCAATCTCAGCTCACTGTAAGCTCCGCCTCCCGGGTTCAAGCGATTCTCCTGCTTCAGCCTCCCGAGTAACTGGGACTACAGGCACCCGCCACCATGCTCAGCTAATTTTTCGTATTTTTAGTAGAGACGGGGTTTCACCGTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCTTGATCTCCTGACCTTGTGATCCGCCCACCTCGGCCTCTCAAAGTGCTGGGATTACAGGCGTGAGCCACTGTGCGCCCAGCCCTTTTTTCTTTTTTTTTGAGACAGTGTGTCACTCTGTCGTCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAACCTCCACCTCCCAGGTTCAAGTGATTCTGCCTCAGCCTCCCGAGTAGCTGGGATCACAGGCATGCGCCAGCACGCCTGGCTAATTTTTGTGTTTTTAGTAGAGACAGGGTTTTACCATGTTGGCTAGGCTGGTCTGAAACTCCCGACTTCAGATTATCTGCCCACCTCGGTCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGTGCGCGGCCTGATTTTTTTTTTTTTTTTTTTTTGAGACAAAGTCTCGCTCTGTCACCCTGGCTGGAGTGCAGTGGCACAATCACGGCTCACTGCAGCCTCAACCTCCTGGACTGCAGCAATCTGCCTGCTTCAACCTCTGTAAGTGCTGGGATTACAGACATGAGCCACCAAGCCTGGTCCCATAGAATCTAAACCATCTTGAGATTATTTATCATGCTTAATACAATGTAAATGCTCTGCAAATAACAATTACACTGTATTGTGTGTGTGTTTTTTTTTGATAGGATCTCACTCTGTTGCCCAGGCTGAAGTGCAGTGGTGTGATCTTGGCTCACTGCATCCTTGACCTCCTGGGCTCAAGTAATCCTGTCTCAGTCTCTCAAGTAGCTGGGACTACAGATGTGTGTCACCACGACTGGCTAATTTTATTTTTTTGTAGAGATGGGGTCTCGCTATTTTGCCTAGGCTGGTCTTGAATGCTTGGGCTCAAGTGATCTGCCCACTTTGGCCTCCCGAAGGCTGGGTTATAGGGCATGAGCCACCGTGCCAGCCTACACTGTATTTTTTTTTTTTTTTTTAAGACGAAGTTTTGCTTTTGTTGCCCAGGCTGGAGTGCAATGGCACAATCTTGGCTCACTGCAACTTCTGCCTCCTGAGTTCAAGTGATTCTCCTGCCTCGGCCTCCCAAGTAGCTGGGATTATAGGCGCCCGCCACCACGCCTGGCTAATTTTTTTTTTTTTGAGACGGGAGTCACGCTCTGTCACCCAGGCTGGAGTGCAGTCATGTGATCTCAGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCGCCACCACGCCCGGCTAATTTTTTGTATTTCTTAGTAGAGACGAGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAGGCGTGAGCCACCACGCCCGGCCACTGTATTGTTTTTTACTTGTACTATTTTTTATTCTTGTATTTTTATTTTTTCCAAGTATTTCCAATCTGTGGTTGGTTGAATCTGTGGACATGGAACCCCCATGGATATGAAGGGCCGATTGTATGCTAGTCTGTCTCCTGGAGATGTTTGCAATTTTCCATAGCAAGTCTAAAGAGTGAAGTGTGTATGATCCTGCAGTGGATCCATCATTACACATTTTTGTCCAAACCCACTGAACGTCCAACAGCAAACTGAAGCCTCATGTGAGCACCGATACTGGTTCATTGACAGCAACAGATGGGAAGCTGGGGGCTGGCAGGGGGCACACAGGAACTCTGACCTTTTCACTCAGTTTTGCTGTGAACCTAAAATGGCTCTAAGAAGGAAAGTTTACAAAAACAAAAAAGGAATGGAATACACGTAGAAAGTTCCTGGTGCAGAGGCAGGACTGGCATCACCATCGTTTCCTCCCTGGTGAGCCGCTCCTCCAGGGCACCCCTGTGCCTCCAACCCCACATGTGCCTGCTCCAGAAGCACTTTTGTCTTCTGAGGCTCTGTCTGCGCTCTGTACTCTTCCTGGGATTGGCTCCTAAGTCCATCGCCCCTAACGTCGCCCCATGAAGGACACCACAGTGCCTCCCATCTAGGAACTGCCTTCTCTCCTTTCGCTGAACTCAGACTGCTTCTGGGCCTCTGGAGTTGATATTCCAGCAAAATTGCTTCTAATTTGCTGGCTCTCGCTTAGCAAAGGGAGGTATTAACCGTAACCTGGGAGAGACGCAAAAAGCCCAAGTGACTCGACCCCTCACAACCCTGACTTCAAAGGCAGCCGAAGTCTGTGGGCGACATGTGCTCCTGGATGATGATGGGGTGTGGTGGGGGCCTGGGCTGGTGTTAATCCCAATCCCTGTCCCTGTCCGTACAGGCCTGCACTGGGTCAGAGGGAGTTGTTGAGTCAGCTTCCTGCAGCCCGAGTGTGCAATCTTCTCTGCCAGACGAAAAGAGTCCCACTGCTCTGTTGACTGCTTGAGTCACATCTCAGTCGCAGCGGGGCCTGTTGGTGGCAGGAGCCCTGGAGACAAAAGGCTGTCTGCTCGGGCTGTGGGAGCAGGTTGGTTAAGACTCTGGAATCTGTCCTCCCGCACCCCAGAGGACACCGCCACCTCTGACTCCGCACTGACAACAGCATCTGAAGGCATTTCTTCTGTGGGGCCATCACTTTATTAAGGGGTCATCTAGAAGGTGGGCCCCCTGACAAACCGCGGGACTGTGATCGGGCTCCAGCTACTTCACCACCCCGGGCCAGCCTGCTCCAGGGGTCCCTTCCTGCTGAGAGCAGGCGAGAGGCAGTCAGGCTCATGAAGCAGCCACCGGGTTTGGCTCACTGGAAGGAATCACACTGGAAACATGTTTAGCCCGCAGTGCAGAGTGGCTCCAGAAGGGAGAGGTTCTGGAAGACGCCCCAACCTGCCGGGCTGCTCCCAGAGATGCACAGTGAGGGGCAGGCACCCAGGGCCGTTCCAGGACTCAAGATGGGGATGGAGATGGCGTGAGGAATGGAGGACAGGGCTAGATGGGCTGACCGGGGGGAACAGTGTTACGAAAAGGAGGCGGGTACCCTGGGCTCCCGTGACAAAGTGCGGCAGGGCTACCCCCTGCAGCCCCCATAGCCCCCACCACCTTGAGACCACGTTCTGGTCACTGCCTCGGAGCCCCGATGTGTTGGGGCCAGGGAGCGCTCCTGCCCGGGTGTGGGGTGTGAGCCTCAGCCTCTGTCCGCCCGGCAGCGCGCGTGCCTCCCTTGGTCTGGCCTCCCTCGGCCCGGTCCTGGCACTGGCCGTGCTAGCTGGCCGTGCTTCTCCGCGGGATACAGCCTTCCATCTCCAGCGCCTCGGGGCAGCCTTCGTACTTGTTGCTGTGCTTACTGTTCTTCACGTGCTGTGGGGAGGGGAGCAGAAAGTCATGACTTTGGCCCTCAGGGACAGGGGACAGGAGTGGACCAGGACCCAGGCTGTCAGTCAACTGTGCTGTGTTGGCCAACGGTGTACTCTCAGGGCACTTGGCCCCTCCACCCATCCCAGGGAAACATGGCATCAGCTGCACTGGCCCACCCCTCATAGCTGGACTGGGGACGGTCCCCTGCTGGGGCGTGAGCAGCTGGGGAGGCTCGTCGCATGCAGGCAGAAGGGGCGACAGGCTGCCCCGGTGGGGGTGATGCCAGGCAACCCCCCATACCTGCTCAAAGGGGCTCTTGTTCTGCACACCCTTGGCCCCGACAAACACCCAGCTGTCCCGGAAGGCCAGCTCCTTGGCGTTCCTGCTGCCCAGCTCACTGAAGAGCTTTCTGGTCTCTTCATTCATCCTGCAGCATGGGAGGAAGGGGTGTCAGCTGTTGCTGCAGAAGGGCAAGGCTGAGGCTGGCCTCCCCAAGCACCTACTTGGTGGCTGGGTCGTCGTAGGATGCCACGAACACCAGGGTGCCTTCGTGCAGTGGCCGAATAAACTTCAACAGGTCGTTGACATCTGGGGGGGCAGGTGCCACGGAACAGGGGTCATCAGGCACCACTGAGCACCCTCCCACAAGCCCGTTCTCCAAACAAGGAAACAGAAGTAGGGATTGGGCCTGGGACATGCCCCGCCAAGGGACAGGCTGGTGTAGGAAGCTGAGGCATCCCCGTGACCTCCCACAGCCTCCAGACCAAGCTGGCGATGCCCCTGTCCTTTCTGAACCCACGGAGGTGACTGAGAAGCTCCAAGTACAGACAGAGAGAGCAGTGTCTCAGGGGAAGCTGCCCAGGTCATTTTGCTGCAGTCAAAATGCAAGACGCTGCGCTGCAACTCACCTCCGGCCCACATGTCAAAGGCCCGGGCCTCGATGAGCTCGCCGCTGACCCCTGTGTCAGGAGGGAGGGGCCCTGCTGGGTAAGCCAGGCCAGCCTCAGGGCACGGCAGGCGGGTAGCACCGGGGGTGGGGGGCAGCCCTTCTGGACGCTTTCAAACAGTCCCCCAATATTCATCCATTTGTTCAGGCAGCTGCATCCCACCCCAAGCCGTGGTGCCTCTGTCTGGCATTTGAGGCCCTCCGGGGGCTCTCCATGAAGCACCCTCAACCTCCCAGAACCTTCCCTAGTTCTTTATTGCCATCATGCTTTTTTGTTCATTGGGTCACTCCTCTGTGGAGCCTTGCGCTTGCACAGACTCCTTCCCAGGCCTCCCTGGGAGATCTTGAATGGGAGGGGAAGGGAGGGCAAACACCCAGAGGCCTCCTGGGGAGGGAGCAGGGAAGGAGGGCGGCAGGCCACGCTCAGCCAGGGGCCTCACCGTTCACCAGGGCGATGTTCAGCCCGCGGCCCACGTTGTCCTTGACGCTGCTCATCAGCCTAGTTGGGGGGGGGTGGGGGGGACGGGGAGATCCCACATGGGCACGTCTGCTCCACTCTCCCTTGCAGACCCGGCCCTGGATCCCCCACTCCCTCCCTGATCCCCAGTCACCCCAGGGAGCTCACATCTTGTCCTCGAGGCAGATCTTGGGCCCAATGACGTTGGCGGCCCCGCTGACCACGCGGAAGGCCAGGTGCTCCTCAGGACACGGCTGGGGCAGGCCACACTTGTACTTCCTGGCCCGTGGCGCTGGGCAGGGATAGCAGGTGTTATCCATGGGCCTGGCCCTGAGGTCACCTGAGATCTGAAGGGGAGGTCAGGACCAGCACACATGGTAGAACAAGTGACCGCAGAGCAGGACACAAGTAGGGGGATGTGGCTCAGGCCGAGGCTGGGGACACTTCCATACTCTGTGTCCTACTGGTGAGGCCACAGGGACCCAAGACAGAAGGATTTTCTGTTACTGGTGGGAGAAAAGAAAGGATGCTGAGGGCTGGCCTGGGCCACTGCACAGCCAGGAACAAAGCAGGGGTGAAGTAAGTCAGGCTGGGGGTCTAGCTGCATGGATGAGGCCAACCTGAGCAAAGGCAGTGTGGCAGGGGGTAGTGTGGCAGGGGGTGCAGCCTGTCTCCCGATTTTGATGGGGGTTGGTTGGGAGGACTGGAGCAGGATGGCTTCTATTATGGGTTGAATTCTGTCCCCTCAAGGTATGCTGAAGTTCTAATCTGTGGTACTTCAGAATGTGATGTTTGCAAATAGGGCCTTTGCAGTTCAGATGGGGTCATACTGGAGTAGGTTGGGTCCTTAATGCAACATGACTGATGTCCTTATAAGAACAGAAGTGACAAAGACACACAGAGGGAAGACAGCCATGGTACAGTGGAGGAAGAGACAGGAGTGACACAGCCAGAGGCCAAGGTATGCCTGGGGCCACTGGAACCTGGGGAGGTCAGGAAGGACCCTCCTGTAGAACTTTCAGGGTGAGTAACGCCCTGCTGACACTTTGATTTCAGACTTCTGGCTTTCAGAATGGGGAGAGAATCCATTTCTGTTGTTTAAGCCACCCAGTTGAAATGGCAGTTCCAGGAAATGGATACAGGTTTTGGCACCAGGAAGTGTGGTGCTTAGGTAACAAATACCTAAAAATGTGGAAATGGCTTTGGAACTGGGTAGTCAGTAGAGGCTTGTCTTAGTCCATTTGCTGCTGCCATAACAGAATACCACAGACTGGGTAATTTATAAAGACAACAGATTCATTTGGCTCATGGTTCTGGAGGCTGGGAAGTCCAAAAGCATGGCGCCGGCATCTGGTGAGGGTCATCCCGTGGCAGAAAGGTGGAAGGTGGAAGCAAGCATATGAGACGCAGGAAAAAAGGGGGCTGAACTTCAAGGTAACTAACTCACCCATAATAATGGCATTCATCCATTCATGAGGGCAGAGCCCTCATGACGTAACTACCTCAAAAAGGCCCTCCTCTCAATACCAATACATTGACAAATTTCAACATGAGTTTTAGAGATGACATTTAAACCATATCAAGGCTATAGAAGTTTGAGGTACATGTTAGAAAAGGTCTGGATTGCCTTTAAGAGACTGTTGGTAGGAGTATGGATGTTAAAGGTAATTCTGGTGAGAGGAGGGGAGAGCAATAGAGACAGCCTCTGTGGTCTTAGAGAATACAAATATCATCAAGAACAGAACGTTGCTAGAAACATGAATGTTAAAGGTGCTTTTGGGTTGGGTGTGGTGATTCACGCATGTGATCCCAGCACCTTGGGAGGCCAAGGCAGGAGCATCACTTGAGGCCAGGTGATCAAGACCAGCCTGGGCAACATAGTGAGACCCCGTCTCTACAAAAAATACAAAATAAGCTGGGTGTGGTGGTACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAGCTATGATTGCGCCACTGCCCTCCAGCCTGGGTGAGAGAGCAAGACCCTGTCTCAATACATTCAAAAAATAAATAATAAAAAATAAAACATTAGCTGGGTGTGGTGGCACATGCCTGTAGTCGCAGCTACTTGGGAGACTGAGGTGAGAGGATCGCTTGAGCCCAGGAGTTTGAGCTTACAGTGCACGGGGATGATACCACTGAACTCCAGCCTGAGCAACAGAGTAAGACCTCTGTCTCTACCAAAAAAAAAAAAAAAAAAAAAGGGTGCTTTTGGTTAAGCCTCAGAGGAACTGGAGGGACATGTTATTGAGTTATTGGGCGCTGGAGGAAAGCTGATCCTTGTTATAAAGGAACTTGGCCCAGGTGTGGTGGCTCACGCCTATAATCCCAGCACTTTGCGGGGCCAAGGCGGGAGGATCACTTGAGGTCAGGAATTCGAGACCAGCCTGACCAACACGGTGAAACCCCATCTCTACTAAATATGAAAATTAGTTGGGTATGGTGGCAGGTGCCTGTAATCCCAGCTAATCAGGAGGCTGAGGTGGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGATGGCTCCACTGCACTCCAGCCTGGGTGACAGAGCAAGACTCCGTCTCAAAAAAAAAAAAAAACTTGGTTGAATTGTGTTCTAGTGTTTTGTCGAAAGGAGAATGTGTAAGTGCTGGGTGCTGAACTTGGATATTCAGCTGAGGAGATTTCCAAGCAGTGTTGAGAGCGTAGCTGACTTCTCCTTGCTGCTTATAGTAAAATGTGAGAGACATAAGGAAGAAACTGTTGAGCACAAAGGAGCCAGAAAGCGAAGATGTGGAAAATTCTTGGCCTAATCATATTACAAAAACTGCAAAAGTGTCCCCTGGACAGAACAGCAAGGCTGTTCAAGCGTTTGCCCCAGAGCTGAGGTATGTGACTCGTGGGTCCACTCAACCCTTTCAGCAGAAATCTACAATGAAGATGGGGTTATCTAGGAAGCGTCTGTGGGAAAACTCTCTGATTTTATAGCTTGGGCCTCTATAACTTGCAAAAGAGACCAACAAGGCTCTTGAGAATCTGACACCAGCAGAAACACTGCCGGCCTGGAGTGAAGGGGGCAGAGATGAGATAAAGGGAAGGAAGGGTGGCCCCGAAGGTGGGGCTGTCTCGTTTCAGAGCATGGGTCATTCCAGCAGGCCCAGAAGAAAGAATCTCAGGCCACAGAGGATTCCCCTCAGGCCTTGAAACCAAATGAATTTTGCCCTGCTGGGCTTTGGACTTGCTTGGGACGGGTGACCTCTTTTTTCCTTCCAAGTTGCCCTTTTGCAGTGGGAGTATCTATCCTAGGCCTGCCCCACCGTCATATTCTGGCAGGAAGTAACTTGGCAGGAAGTAACTTGTTTTGTAGGTTGACAGTGGCAAGCAGCCCCCGTGGACCACCACAGCTGGTTTGGGGAGGAATAAATGGAAAACCGAAGCCCTACGGGACCGGGGGCAGGCAGGGATGTCACAAAGGGGAGGAGCAGGGAGGTGACAGGGGCCTTACCTGCAGTCACCGAGCTCTCTGGACCTGTGGATACAGAGTGGTTTCTGTTAGTGTGGAGCCTCCCGGGGCCTGCGGCAGGGCAGCCTGGCCCTCCTTACACCGCGAAGTGTAGACACAGGCAGTCAGGCTCAGGGGCCGGGGGCTAAGCCAGGCCGACCTTGGCATGCTTCCTGCTCAAGTGTAAGTCATGTTCAGATGAAAAACTCCCTACTCTAGGAACAGGGGGAAATGGAATTAGAAAAACTACTTAGATGACCTGGCCGGGCATAGTGGCTCATGCCTGTAGCACTTTGGGAGGCTGAGGCGGGTAGATTGCTTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACATGGTGAAACCCCATCTCTACTAAAAATACAATAATAATAATAATAATAATAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCCGGGTTTGGTGGTGCGCACCTGTAGTCTCAGCTACTCGGGAGGCTGGGGCACTAGAATCGCTTGAACCCAGGAGGTAGAGGTTGCAGTGAACCGAGATTGCACCACTGCACTCCAGCCTGGGAGACAAAGTGAGACACTGTCTCAAACAAACAAACAAAAAAGTACTCAGGTGACCTATTACCAGTCAACAAGGTGGAGAGTCCCCTCTCCATCTCAGAGCCTGCAGGGAGCAACCGGGGCATCACTCCCCATGTTCAGTGGAAGCTCCCTACCCCTTACTCTGGCCCTACTGACTCCAGTTGGGCATATAATTCTAGTGATCAGGTATTGGCTTTCCTAGATGAAGCAACTGGGTGTTGGGGGAGGCTGCGCCTGATGCCAGGAGACAGACTGGGGACAGCCAAAGGTAGCCTGCCAGGGAGGAGATAGAAAACCCAAGTCAACTCCCAACCCAGAGCCTCAACCACAGGCCCAGGTGGCCACCTTGGTGGCCTCCAGAGAAGGATAAGGCGGGGTCCCACACTCACTGGTGAAGAGTTGCTGGATGCGAGGAAAGCCACTGCCAGGCCCACCCAGGAGGATGCTGACCACGATCCATGTGACACCCACACTGACGACTAGGACCACAATGCGGAGAGGGCCTGGAGGCAGGATAGACACAGGGTGGGGTCACCTCAGATACCAGCGAACCCCAGAATGACCCCATAGCCTTGCATGACTGGATCTCCCCTGCCACTTGGGAAGAAGTGGCTGGAGGACAAGGAGGTGGAGACCTGTTGCCTTCTACCAGGGCTGAAAGGGAGACTTGAGCTCCCTGGCAGGTGGAAGGAGGAAATTGAGTTCAAATCCCAAGGCTGCTGGGTTTGGTTTTCCCTGGCGGGTCTGGGAGGGGGCCTTCCAGAACTCTCTGCTCGAGAAGGGCTCCAGCCCTCTTTCCAAGAACCCCCTCAACTTCCTGCCTCTGAAACCACCAATATATTTGGGCATCCACCTGCCCCTGCTTTCCTCCTGTTCTGAGGGAGAAGGGTCCCTACTCCCTTTTAAGACCAGTCCCGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGAACTTTAGGAGGCCGAGGCGGGTAGATCATGAGGTCAGGTGTTCGAGACCAGCCTGACAAAGATGGTGAAACCCTGCCTCTACAGAAAATACAAAAATTAGCCGGGCATGGTGGCGCGCACCTGTAATCCCAGCTACTCAGGAGGCTGAAGCAGGAGAATCAGTTGAACCTGGGTGGCGGAGGTTGCAGTTAGCTGAGACCATGCCACTGCACTCCAGCCTGGCGACAGAGTGAGACTCTGCCTCAAAACAAAAACAAAAACAAAACAAAACAAAACCAAAACAACAACAGTCCCGTCATCTGCTGGGACCTGGATTGCAAGCTCCCCAGCCTTCTCTGATTTCTTGTAATAGTGATCACTCTCTCTAGTTCGCTCTTTCTCCTGTATTTTCAACATCTCCAATGTTAAGGAAGCAAAGAAAACACTCTTCTTCAAAACCACATCCCCATCCTCTTTCCCCTAACCTTTCTTTTCCCTGTCAACCCCCTCAAAAGTCATCTATACTCATTCCATCATCTCACCTCCTACTCCCTCCCACCCTACTCCAGTCTGGCCTGGCTTCCACCACCTCACCAAATCAGCTTGTTGCCAAGGCCACTGATGACCTTTGTGGTGTCACCACCCCTGGTGACCTGTCAGCCTTGATCTTCCTTGACCTCTCAGTGGGCTGCCCATGTCCACCTGACAGAAGCACTAACTTCTCTGCTGGCTTTCCGGACAGCACACTCTCCTGACCCAATCTCTCTGGACACTCCTCATTCTCCTTAGTTAGATCGTCTCCTCTACCCTCCCCTTAAAAGCTGGACTTTCCCAGGCTCCCATCTCAGGCTGCCTTCTGGTCCCTACCTGTAGTGTCTTTTTATTTTTTTTATTTTTGAGACGGAGTCTTGCTCTTGCCCCCCAGGCTGGAGTGCAATGGCGCGATGTCGGCTCACTGCAACCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCGCGCACCACCGTGCCTGGCTAATTTTTTTTTTTGAGACAGAGTCTTGCTCTGTTGCCCAGACTGAAGTGCAGTGGCGTGATCTTGGCTCACTGCAAGCTCCGCCTCCCGGTTACACGCCATTCTCCTGCCTCAGCCTCCCTGGTAGCTGGGACTACAGGCGCCCGCCACCACGCCCGGCTAATTTTTTTGTATTTTTAGTAAAGACGGGGTTTCACCATGTTAGCCAGGATGGTCTTGATCTCCAGACCTCGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCCGGCCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATCTTGGCCAGACTGGTCTCGAACTCTTGACCTCAGGTGATCCACCCGCCTTGGCCTCCCAAAGTGTTGAGATTACAGGCGTGAGCCACTGCGCCCAGCTGTCTTTACTTTTTTGAGACGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTTATGCGGTGGCGCAATCTCGGCTCACTGCAACCTCAGCCTCCCGGGTAGCTGGGATTACAGGCACCCGCCACCACGCCAGGCTCATTTTTTTGTATTTTTTTAGTAGAGATGAGGATTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACTTCAAGTGATCCTCCCACCTCGGCCTCCCAAAGTGCTAGGATTACAGGCGTGAGCCACCGCGCCCGGCCTGTAGTGTGTTTTTAGATGATGTCATCTACTCCTAGCTCTTTAATTAAGCACCATGTGTCAATGACTTTCTATTTTCTAGTTCCAGCCGTGGCCTCTCTTGAAACCACTCCCTGCCAGCTGGTTCCTCAGTGGCCAGCTCCACCTGGATATGTCCACAAAGTTACCACCTTAGCAAGTCTGAAGTCCAATTCCCGAGTTGTTTCTACTCTCCACCCAGCCCCAACCTATCCCTCCCCAAGGATCTTCATCTTACTTGTCCAGGTGACAGTAGGGCTGTCATCGGGTTCATGTCGAAGCCCACAGCCACTTTTGTCACTTCCCCTCTAAGACCACGCCTCTCCACCTGGACTGCCCACCTCCTCTCTCTCACCACTCATTTTCCAAAAAGCAGCTGGTCTGCTACCTGCGTTGGGAGATACTGACTATACTAATCCTCATGTCATAGCTCGGGGAGCTGACGTCCTCAGAGGTGAAAGGATTTGGTTAAGGTCAAGGAACGAGTGTTTGTGCAGAGTCCTCCATTTCCAAAACCAAACCCAGTGGTTCACCCGGGCCTCTCCAGACACAGATGTCGCGACGCTGGGCCTCCAGCCAGGTGCCCTTTTCAACTCCCTCCCGTTCGCTGTGGAAGCCACCCACCTGCCAACCTCATGTCCACTTCTCCTGCTGGGTTGGGACCGCCGGCAAGTGCACTGTTTGGGGGCAAAGCGGAAGGACAGGTTTGGGTGGGGGTCAGGGGCAAGCCTGTGCGGGACCCCTGCTGGGGGCGGGCGCGATCGGTGCTACGACCTGTTTGTCCAGCCGCCCGGCCAGGCAGGGCTCCTCGGAAACGCGCGGGGAAACCCTGCCTGGCCAGGAGGGGCCTCAGGAACCCGTTGGCTCACGATCTTGCCCACAGGAGCCTCCGGGGCTGGGACGAAGATGTGGTTCTCCTGGGCTCGGGCCGTTCCTCCGGGCCTGGGGGCTGGCGATGCGGGCCGGGCCGGAGATTTCTCTGTCCGTGGGGAGACGTGGGCTCGGTCAGGGAGGTTCCCTGTCAGGCCCGCCGACCCGCTCCGCCCCCGGAGAGGACGCAAGGCCCCTGCGCAGGGTGGCCAGGCAGGCCCGGCAATGGCGGCTAAGGGCGGGGCCACAGCCCGCCGGCCCCGCCCCCGGCAGCAGCTGCGCCGTCTGGCTCCACGCAGGCCCCGCCTCCAGACCCGCCTCTACCGCTCGCGCTGGCCGCCGCATGGGAGGGGCCTGAACCTGTCCCGCCCCGAACCTGTCCCGCCCCTGGATCCCTCGTCGGCCCCACCTCCGCGGTGCGTTCAGGCTGAACCCACCGGCCGCCGAGTGGGAGGGGCCCACGTCGGTCCCGCCCCGGAAGTCTCCCCTCGGCCCTTTCTCGGGTGGGAGAGCCCTGCCTACCCAGGCTTAGAGGGCTGGGGCTGCGCTTGCACCCTGAGGTCAGATTTGTGAGTCGTGGGAAGACGCAGATGGATCTGGGGCAGAATTAGAACCTGTTCCAAAGTTGATCCATGCGCTTTGTAAAGGCAAGGGAGGTACAGAGGCGCAGATAGGGAAGACCGCAGGATCCAGCGTGAGCCTCTGGCTGCCTTTCCTTAGGGTCTGTTTTCTGTCCAGAAGCGCGTTCCTGCTGTCTCTCGTTATCACTTTTATAATGGGACGGGGCGTCTCAAACTATTTCGACATCCTTGAAAACTCTTGCCTGCTCCGCCTGCCTGGTCTCTAACACCCTCCTCCTCAGAGCTGCTCAAGCTGTGTTGTTTACGCCACACCCCACCCTAAAAGCAGAGGAGAATACAACTTTCGGGGGCAGTGGAGGGGGGAGTTTAGCGCTATAACCCGAGGCTTTCACAGCAAACCAGAATTTATTTTGAGGCCTCTCAGGTTCAGCTTTCAAGTCACGTGACTTTCGCGTCCTACACAACTCTTATTTTCACTTGAAAACAAGTGTGACGTCGTGAATTCCCTAACAATAGGAAAAAAGAAGGCTCCAGAAAGAGCGGTGCTTGGCTTTCCCAGCGGCTCCAAGCGGTGCCCGGCACCCAGCTGGCTGTATGGACCTGGGGTGGTCTCCGGCCGCAGGCAGACGCGGCCCTTCGGCCTTTCTCCAGTCACCCCAATTCTCCCTGACTGCCTGGCTATCTCCCCTCCGCGCCTGCCCGCACGCTGCCCCCAACACTGCCCGGGCTCCCTCTGCCCATGAGAACACGGGTCAAGCTCCCGTCCCTTCTGCCCTGCCGGCTGCCCCAAGTGCGCCCTGACCACCTACTGCGCTGGCATGGCCGGGGCCACCCTGACAGCTGATCCATCATCTGTCTGGAGAGCAGCAGCAGGGCCCAATGAGAACCACGGAAACCTCCCTGCCCTTTGACCCAACTTACGGGAATCTCCTACCCTTAGGGAAACAATGCAAAATATAGAGAAAGTTTATAGAGCCGGGCATGGGAGAGCCCTGGTTAGAATCCAGCAGCTTCTATACTGGAGTACTCAACAGGGTCAAGGTCCCTTTCAGCTGCCTGCTGCTGGGGCCAGGGCTTTGCATTTGCGTTTTGTCATGGAATTTGACAGCCTCTCAAGTAATTATGGTAACGAATCTTGGGCTCCATGCCCTCCTCAGGGGGGCCCAGTCGAAGAAGCTCCAGGTTACTCACACCCTTGAGCTGCCAGCCGGTGTTTTTCAAAGACAATATTTACATGTTGTTCAGAGCAGGCATTTATGAAAGTGTTGCTTTAATGAGTCCCCCTGAGCATTCCGAGACAACTCTCCCTTCCTTGGGGACACCACGTGGGTGATGGGCCAGAGGCCTGGGATTCAGTTCCACGTCTTCCGTGCTTCCTGGGGCAGCCTTGCAAAAGTCCCTTATTTTCTCTGGGATGGTTTGGATTCCTGTGGGGGTCTGGCCAGCACGGTGACTAGAGCACATGGTCTGTCACCATCCAGGCCAACGTCCTGGGGTACACTCACAGTCATCTGTTCTTCCAGGTCAGCCCTCATAGATCAGGGCAGCTTCAGCAGTTCTCAAATAGCAGATGTTACAAAATGGGGGTGGGCAATCCTGCAGCAGACGTCTCTGCACTTTCTGTTAGAAGGACACCCCAGGTATTTTTGAGCACTGTTCTGAAGACGGAAAAATCCAGAGTATACATACACTTAAAGCTGGGAGGGGCACCTGCCGGGGAGAATGCATAAGGGATAGAAACAAAAACAGAGGGCCGAAGCCTCTGCCAGGCAGAAGACACTGAGTTCTCAGCAAAGAGAACACCTTTCAGTGCTGTGGTCAAAGGGATGTCAAGCCTCCTGAGGCCCGGAAAGAGCAGGAAACTGAGGGCAGAATTGGAAAAAATCAAGTTACAGACACACACCACGGGATAACCTCTATACAGAGAGGAGCACACCCAAAATAATACTATATATTTATAGATGTACTTTTGTATTGGGGTAAGACATATATAACATAACATTTGCCATCCAAATCATTTTAAGTATACGATCCTGTGGCATCAGTTACATTCACAATGTTGTACAACCATCACTACTATCTTTTTGTGTGTGTGTATATTACTTTTTTTTTTTTTTCCCCGAGACAGAGTCTTGCTCTGTCTCCAGGCTGGAGTGCAGTGGCTCAATCTTGGCTCATTGCAACCTCTGCCTACCAGGTTCAAGCAATTCTCCTGCCTCAGTCTCCTAAGTAGCTGGGATTACAGGTGCCTGCCATCATACCCAGCTAATTTTTATATTTTTAGTAGAGACGGGGTTTCAACATGCTGGCCAGGCTCGTTTCAAACTCCTGAGCTCGTGTTCTGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGTGCCCGGCCATGTATTACTTTTATGGGCTCATATATATGTCCATAGAAATATAGAGTTCTTTTAAAAATTCTATCAATAGATATTTCTTTTTCTCTTTTCTTTTTTTTTTTTTTTTTTTGAGTCAGAATCTCACTCTGTTGCCCAGGCTGGAGTGCAGTTGCGTGATCTCGGCTCACTGCAACTCCTGCTTCCCGGGTTCAAGTGATTCTCCTGCATCAGCCTCCCGAGAAGCTGGAACTACAGGCGTCTGTCACCATACCCGGCTAATTTTTTTGTATTTTTAGTAGAGATGGGGTTTCACTAGTTGGCTAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAGGCATGTGCCACCATGCACGGAAATCAATAGATATTTCATTTCCACCAATACAGAAGCATCAGTTAACAGTGACTGGTTGACACTTTTAGAAAGTGGCTTGAGAAGGGCCCTGAATGAGCCACCTACGTGGAACACAGAATTTGAGATGTGCAAATGTGGAGACCTGAAGGCTTGTTATGGGAACAAAAGGTGGCTGTGTATAGAGAGGAGCGCAGATAAAGGGGACTTATCAGCAGTCGCAGGGAGTTCATGCTCAACAACTGGCAACAAGCGGGGCAGCTACAGGCTACTCAACTGGGGATTGATGCGCGGTGCCTCTGAGGTTCAGCTCTGGGCCTGATCGTCCAAGAAAGGGCAGCTAAGGTGGGCATAGTGAATCACGCCTGTAATCCTAGGACTTTGGGAGGCTGAGGTGGGAGGATTGCTTGAAGCCAGGAACTTGAGACCAGCCTGGGCAACAAAGTGAGACCCTGCATATATATATATATATATATATATATATTTACACAAATAAATAAAGGGCAGCTAGACTCCACAGAAACCATGTTGAAGATTCCTGTGCAGGAAGGGGGTGATAAAATGCAGAAACCTGACCTGACACAGCCAACACATAACTGCATTGCCTCATTTTTCACGATTTTTTTCTTTCTTTTTGGAGAGAGGGTCTCATTGTGTTGCCCAGGCTGGAGTGCAGTGGCGCAGTCACAGCTCACTGCAACCTCTGCCTCCCAGGCTCAAGCGATTCTCCTGCCTCAGCCTCCTCAGCAGCTGGGATTACAGGCATGTGCCACTACTGGCCGGCTAATTTCTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGGCCTCAAATGATCCACCTGCCTCGGCCTCCTAAAGTGCTGGGATTACAGGCGTGAGTCACCACGCCCAGCCATTAATTGCCAAGTCTCATTGGCTCCTAGTGTCTTAATCCACATCTGCTAAAGTTGTTTAAGGTACAGCTAAAACATCACCCAGGCTGGGCTCAGTGGCTCACACCTGTAATCCCAGTAATTTGGAAGGCCCAGGCGGGAGTATCACTTGAGCCCAGGAGTTCGAGACCAGCCTGGACAACACAGGAAGACCTTATCTCTACCAAAAATACAAAAATTAGCAGCATGTGGTGGTGCACACCTGTGGTCCCAGATACTTGGGAGGCTGAGGTGGGAGGATCACTTGAGCCCAGGAGGTCAAGGCTGCAGTGAGCTATGATCACACCACTGCACTCCAGCCTGGGCAGCAGAGCAAGGCCCTGTCTCAAAAAAATAAAATAGGGGCCAGGCACGGTGGCTCACGCCTGTAATCTCAGCACTTTGGGAGGCTGAGGCAGGTGAATCACCTGAGGTCAGGAGTTCAGGACCAGCCTGGCCAACATGGTGAAACCTCGTCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGGTACACGCCTGTAATACCAGATACTCGGGAGGCTGAGGCAGGAGAATCGCTTTAACCTGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGTGCCACTGCACTGCAGCCTGGGTGACAGAGCGAGACTCTGTCTCTAAATAAATAAATAAAATAAATAAATAAATAAATAAGGCTGGGTGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGTGTAGGGAAAAGAGAGATCAGACTGTCACTGTGTCTACGTAGAAAGGGAAGACATAAGAGACTCCATTTTGAAAAAGACCTGTACTTTAAACAATTGCTTTGCTGAGATGTTGTTAATTTGTAGCTTTGCCCCAGCCACTTTGACCCAACCACTTTGATCCAATCTGGAGCTCACAAAAACATGTGTTGTATAAAATCAAGGTTTAAAAGGGACCTAGGGCTGTGCAGGACGTGCCTTGTTAACAAAATGCTTACAGGCAGTATGCTTGGTAAAAGTCATCGCCATTCTCTAGTCTCAATAAACCAGGGGCACAATGCACTGCGGAAAGCCGCAGGGACCTCTGCCCTGGAAAGCCGGGTATTGTCCAAGGTTTCTCCCCATGTGATAGTCTGAAATATGGCCTCGTGGGATGAGAAAGACCTGACCGTCCCCCAGCCCAACACCCGTAAAGGGTCTGTGCTGAGGTGGATTAGTAAAAGAGGAAGGCCTCTTGCAGTTGAGATAGAGGAAGGCCACTGTCTCCTGCCTGCCCCTGGGAACTGAATGTCTCAGTATAAAACCTGATTGTACATTTGTTCAATTCTGAGATCGGAGAAGAACCGCCCTATGGCGGGAGGCGAGACATGTTTGCAGTAATACTGCCTTGTTATTCTTTACTCCACTGAGATGTTTGGGTGGAGAGAAACATAAATCTGGCCTACGTGCACATCCAGGCATAGTACCTTCCCTTGAACTTAATTATGACACAGATTCTTTTGCTCACGTTTTTTGCTGACCTTCTCCTTATTATCACCCTGCTCTCCTACTGCATTCCTTTTTGCTGAAATAATGAAAATAATAATCAATAAAAACTGAGGGAACTCAGAGGCCAGTGCAGGTCCTTGGTGTGCTGAGCGCCGGTCCCCTGGGCCCACTATTGTTTCTCTATACTTTGTCTCTGTGTCTTAGTTCTTTTCTCAGTCTCTCGTCCCACCCGACTAGAAATACCCACAGATATGGAGGGGCAGGCCACCCCTTCACCAAGGGGCCAAGGCAGGCGGATCATGAGGTCAGGAGTTCGAGACCAGCCTGGCCAAGAGACCAGCCTGGCCAATATGGTGAAACCCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCGGGTGCCTGTAATCTCAGCTACTGGGGAGGCTGAGGCAGGATAATTGCTTGAACCCAGGAGGTGGAGGTTGTGGCGAGCCGAGATCATGCCACTGTACTCCAGCAGCCTGGGCAATAAGGGTGAGACTTCATCTCACCAAAAAAAAAAAAAAAGCAGCTCCACTAGGAAGACTAAAATACTAAAATGATGGTGTGCTAACTTGAACAGTTAGGTTGTACAAGAAAGGACTTGACAGTTCCCTTTCTGATGTCCTCCAAGACATGGGTTCCATTTATATGCACCTTGTTCTTTCCCTCAGACCTGTAACTTCAGCCTGGAGTTGAGCAGAAACATGGCTTCCTTGTCTTCAAGTCATTCTTGGGCTTCAGAGCGAAGATGCTGGACCTTTGAACCAACAAGCAGGTTACTGGTACCTTTGCCCTGAGAATACGCTGGTGGTGCTTGTGGCTGCAGTGTTTACCCCGAGATAACTTTGCCATGAAGTATCTTCCTTTTATTATTTTTTCATCGCTCTAGTATATCGACTTTGGAAACAAAAGACATCACTCTATTTAGAGCATTCCTTTCTTAGTAGTGGTATTTCCATTGACAAAAAAATAGTAATTCTGAATTGCCGAAAATGTCAAATCGTAGAAAATGTTGTTAGCCGAAGATTCATCTGATGAATCAGATTTTTCCAAAATAGATGATTCTGATGTTAGTTCTGTTTAGAAATAACTCCAAGTACAGTTTTTATATATTATTTTCACATTGAAAATCAGTCAGATTTACTTCAGCCTCAAAGAGTGTGTTTATGTAAAAATAAATGAGCACTGGCAACCTCCGCCTCCCGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCCAGTAGCTGAAATTACAGGCGCCCGCCACCACACCTGGCTAATTTTTGTATTTTTAGTAGAGACGAGGTTTCACTATGTTGGCCAGGCTGATCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATATATAGGTGTGAGCCACGGCACCCGGCTGGCTTTTCAATGGTATATGGGAATGAGCTGCTTGGTCAGCTGGATAAATGTACTGTCAGCCTCAGGACTGCCTGCATTCACCCTGGGAAGCAGTCAAACCCCATGTGATGGGTCATTGGGTCATGAGATGCTGGAACATCACTGTATTGCTGGCTTTGGAGAAAGATAGATTTTTTTTTTTTTTTTTGAGACGCCGTTTCACTCCGTTGCCCAGGCTGGAGTGCAATGGTGTGACCTCGCCTCACTGCAACCTCCGCCTCCCGGGTCCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACCCACCACCAAGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGATCCGCCTGCCTCATCCTCCCAAAGTCCTGGAATTACAGGTGTGAGCCACCGTGCCTGGCCAAAAGGTAGATCTTGTCTCATTTTCCTGCCAGAAAGCTCCCTGAGGCTGAACATCTGAAAAAAGAACTCAGAAAGACGACCCCAGACATTTGCTGTGTACAGCGCTACCCAGAATTATTTGAAGAAAATGTTCAATTTCCTTCTTAATCTCTTCACTGACTCACTGGTCATTCAGGATCATATTGTTTAAGCAGAAAATGTTTGGGAAAAAAAAAAAAAACCACCACCACCACCACTAAAAAATAACAGCACTGGGTGGGTGTGGTGGCTCATGCTTGTAATCCCAGCACTTTGAAAGGCCAATGAGGGCGGATCACCTGAGGTCAAGAGTTCAAGACCAGCTCGGTCAACATGGCAAAATTCTGTCTCTACTAAAAATACAAAGATTAGCCAGGTGGTAGTGCGTGCCTGTTAATTCCAGCTTTTCAGGTGGCTGAGGCACAAGAATCGCTTGAACCAATGAGGTGAAGGTTGCAGTGAGCCGAGATTGTGGCCACTGCACTCCAACCTGGGCTACAGAGTTGGACTGTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAGCAAGACAGTGAAACAACTATTTACGTAGTACTTATATTGTATTAGATACTATAAGTTATCTAGAGATGATTAGATTATTTCAAGTGTATGGGAGGACTGGGGAGTTCTGCACTTTGGGAGGCTGAGGCAGGAGGATTGCTTCAGCCCAGGAGTTCAAGAGCAGCCTTGGCAACATGGCAAGATCCCAACTCTATTAAAAAAAAAATACAAATTTAAAAAGTACACAGGAGGGGCCGGGCACGGTGGCTCACGCCTATAAGCACTTTGGGAGGCCGAGGTGGGCGGATCACGAGGTCAGGAGTTCGAGACCAGACTGGCCAATATAGTGAAACCCCACCTCTACTAAAAATAAAAAAATTAGCTGGGCGTGGTGGCATATACCTGTAATCCCAGTGACTCGGGAGGCTGAGGGAGGAGAATCGCTTTAATCTGGGAGACGAAGGTTGCAGTGAGCCGAGATTGTGCCACTGCACTCAAGCCTGGGTGACAGACAAGACTCTGTCTCAAAAAAAAAAAAAAAAAGAAGTATACAGGAGGATGTGCGTAGGTTATATGCTGTGCCATTTTATATCAGGGACTTGAGCATCTGTGGATTTTGATACCCCTGGGGGTCCTGGAACCAATCCCCGTGGATACCAAGGGAAGACTCCACTCAACTCTGCCACTACAAACAGCATGTAAGCAAATGGGTGTGGCTGGGTTCCCATAAAACTGTATTTACAAACACCCAATGTCTACTTAGGTGAGCTGCACATTGACCTCTTTAGCTCGTTATTCCTTCTTGCATCTCAGATTGTTCCATCTAGTACTGGGTTTCTTCTGCTTGAGGCACACACTTTTTGGTGAGAAAATGTTGGTGGCAAATTCTTTGCTTTTGTTTGCACTTCACCATCATACTTGAAAGATTTTTTTTTTTTTAAGTCGGAGTCTCGCTCTTTTGCCCAGGCTGGAGTGCAGTGGCACAATCTAGGCTCACTACAACCTCTGCCTCCCGGGTTCAAGTGATTCTGCTTCAGCCTCCCACGCAGCTGGGATTACAGGCAAGCGCCACCATGCCTGGCTAATATTTGTATTTTTATTACAGACGGGGTTTCACCATGTTGTCTAGGCTGGTCTGGAACTCCTGACCTCAGGTGATCCGGCCACCTCGGCCTCCCAAAGTGCTGGGATCACAGGTGTGAGCCACCACGCCTGGCCAGCGACCATCATGCCCAGCCAGCCATCATTATATCTTCAAGTTTTGCTTCTGCCATACTTGCTGTCTTCTGTTTCTGTGAAATCAGTTAAATGTATGGAACACCTTCTCATTCTCCTTGCGGCTTCTTCCTCTGTCTTTAGTGTCTTCCCTGAACTTGTCTTTCTGGGTTGTATTCTGAATACATTCTTCTGAGCCAGTTTTTGGTTCCCGCATTCTCTCTTTGATGTTGCGTAATTTGCCACTAAACCAGTCCATCTACTTTTAAACTTACTAGCTCACATTTTACCTTTTTTGAGACAGGGTCTCACTATGTCGCCCAGGCTGGAGTGCAGTGGCTTGATCTTGGCTCACTGCAACCTCCACTTCCTGGCCTCAAGCGACCCTCCCATCTCAGCTTCCTGAGTAGATGGGACTACAGGCACTCGTCACCACACCTGGCTAGTTTTTGTATTTTTTGTAGACGTGGGGTCTCACCATGTTGCCCAGGCTGGCCTAGAACCCCTGGGCTCAAGCAATCTGTCTGCTTGATCCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCCAGCTGTCCCACCACGACTTATTATTTCATTAGATCTGGTTAAGTACGGTTATTTTATGTCTGGCTAACAATTCCAGCGCCTGAAGACCTCACGATCTGTCGCTATTGCTCATTGTTTCTCTTGCAATGTTGTTTCCTGGTGCGCCCGGTTATCTCTGATGATGTGCTTGCCCTCATGCTTTAAAAAGTACCTGTAGGAGGAAGGAGTCTCTCCTGAGAGGGTTTGCTCTGGGTTCTGTCAGACAGGCAGAACCATCTGCATTGGGGAACTGCCCATCCAGGACCCCTTAAGCCAAGTGAAAGGCTTAAGGTTCCATGGTCAACCCTGGGAATTTGAACCCTGGGAACTTGAACCCTGGGAATTTGAACCCTGGGAATTTGAACCCTGGGAATTTGCACCCTGGCTCAGTTGCAATTCTGGGTTTCCTCTCAGTATGATGGGGCAGCAAGTTGGGGGCCTCAAGATTTGATTTCTGTCCTCTTCGCTTTATAGGGCTGTCAAGGAAAAGTTCCTGGGTGGGCAAAGGCAACTTGGGGGTTAATTTGTATCATGGGGTCCTAGTTTTCTCTGAAAATTTGGCCTTGCAAATGTGTGTGTGTGTGTGTGTGTATGAGTGTGTGTGTGTGTATATATATATATTTATATATATATTTGAGACAGGGTCTTGCTGTGTGGCCCAGGCTGGAGTGCAATGGTGTGATCATGGCCCAAGTGAACCTCCCACCTCAGTCTCCTGAGTAGCTAGGACTACAGGCATGTGCCAACATGCCCGGCTAATTTTTGTATTATTGTAGAAACAGGGTTTCATCATGTTGCTCTGCTGGTCTGGAACTCTTGGGCTCAAGCAAAATGCCCAAAGTGCTGGGATTACAGGAGTGAGACACTGTGTCCAGCCATAGAGTCTCACTGTCCTTACAGAAAGCCAGAATTCAGTCTGCTTCTTGTTCCCTCAAAGCTTCCTCGAAGTGTTCTAGTGGAGGCGAGCAGTGAAGCTCTAGGAGGCAAGAGGCCTTCTCAGGGACCCCAGCTTTGGCCTGTATGTCTGGGTGTCGTCCTGGACTAGTCTGATCACGTCTCACAGCTGAGTTTCTCAGCTTCTCCTCCTGCAGTACAGGAAGGACAATCCCTTCCGACTGCTTCTCACAGGGAGAGGGCAGTGGTGAGCCTAGAAAATAAAGGCCCGAAGCCAGCCCCGCCCCATTTGTGCCTCTCCCTCCTCTCCTTTTCTGCTTCGCAGAGCAGACAATGCAACAGTCGCCCCCGCCCAGCTCAGCCTGAAGACCCCTTCTCCACTCCCATCCCTTTCTGGCTCAGCCACCCGGCAGTGGGCCAGGTTCGGATGGAGGGAGGGAGAGGAGCTGGAGAGAGTTTTAAGTACTCTCTGGGCTTCTCACATTGCTGGCTTCAGTGGTTGCTGGACGCAAATTGATGTGATGTCAGACACCGGCAGCAGAGAAGGAAGCTAAGGCATTTGGGTCTGTGCTGCTGTGGCACCTGGGACTCAGTGTGAAGGGATCAGGCTAGGTTTGAAATTCTCTTCCGGCTTAATAATTTGCAGGTCTGGGCTGGGAGGTAAAGCTCTGAGTACACTGCGAGTGTCAGGGGTCCGGCAGGGGGGCTTTTTCCAGCTCCTACCCACTCCCGCGTGGGGTGAAACCTTGAGAGATGAGACACCAATCCAGGGCCTCTGAAGACCCACGAGGTAGAGTGCACTTAACAGTAAATTGTGGGCTGGGTGCGGTGGCTCTTGCCTGTAATCCCAGCACTTTGAGAAGCCTAGGCGGGTGGATCACGGGGTCAAGAGATAGAGACCATCCTGACCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGTGTGTGCTGGCGGGTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGACCCCAGGTGGTGGAGGTTGCAGTGAACTGAGATCACGCCACTGCACTCCTGCACGCCAGCCTGGCGAAAGAGCAAGAGTCTGTCTCAAAAAAAAAAAAAGAAAAAAAAGAAAAAAAGAAAAAAAAAAAGAGTAAATTGTGGCCAGGTGCGGTGGCCCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGCGGATCACGGGAGTTCAAGATCAGCCTGGCCAGCATGGGGAAACCCCATCTCTACTAAAAATAAAAATTAGCTGGGCATGGTAGCGGGCGCCTGTAATACCAGCTACTCAGGATGCTGAGACAGAAGAATTGCTTGAACCTGGGAGGTAGAGGTTGCAGCGAGCAGAGATCGTGCCACTGCACTCCAGCCTGAGTGCCAGAGCCACACTCTGTCTCAAAAAACAAAAACAAAAACAAACAAAAAACCCGGTAAATTGCGGGGCCGAGTGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGCCGCCAAGGCTGGGGGATCACCTAAGGTTAAGAGTTCGAGACCAACCTGCCCAACATGATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCGTGGTGGCGTACACCTGTAGCCTCAGCTACTTGAGAGGCTGAGGCAGGAGAATCACTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCCGAGATCACGCCAGTTAGTCCAGACTGGGCGACAGAGCAAAACTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGTCAATGGAAGCCCAGGGGTGGGGGTTAACGCCTTTGGCCCCAGTTTTTCAGGGGGTTGAGGTGGGGGGTTTTTGGACCCTGGGGGGTGGAAGTTGAAGGAATTGGTGATGGGGTAATTGAACTCCACCCTGGAAAAAAGGGTGAGTCCCAGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAAATTGTGGCTGAATTCTTTGGCATTGCAGGCAGAGAAGGGGTGAAAACAGGGCTCCAGCCTTGAACCGGTCAGCAGGGGTCAGGGAGGCTGAGTGAGTCACCCTATGGAATGGGCCAGGGTGGATCTGGGCTCACAGGCAAGGGCCTCAGTGCTCCTGTGAGCTCCAAGGGGCTGGGGAGGACTGCCTTCTTGTTCAGGGTACAGCTCGTGTTGTTTTCTCGGGCTTATGTCCATGTCTCAGTGTGGACACCCGCAACTGAGGTGACAGTGGAATGCCCTGACCTGATCTCTCATTCTCTCCCATTGTGGACCCAGTGCTGTCCTCACCTGGGACCTCCTGCCTCCACCCAAGTAGGCGCCCAGAGCTGAAGGGACAGTTGCCTGATGGGTTCAGGTGCCAGTGGATGGTCTTCCACCTCCCGGGTTCAAGCGATTGTCCTGCCTCAGCCTCCTGAGTAGCTGGGACCACAGGTGCCCGCCACCACGCCTGGCAAATTTTTGTATTTTTTTTGGTAGGGACATGTTTTCGTCATGTTGGTCAGGCTGGTCTTGAACTTCTGGCCTCAAGTGATCTGCCCATCTTGGCTTCCTAAAGTGCTGAGATTACACGCTTGAGCCACTGCGCCTGGCCTTACTTGGGTGTTGTTCCCTGCCCTGGAACCTACCCTGCTTAGCCTTACCACACCCCATCAGCCCCCAGCCCAACCCGACTCTCAGCCTTGCCACAGCCCCTTCTGGAGGCCACAGGAACCAGGACAAGAGGACTAAAACCACTGCCCCTGTGGCCGAAGCCTGGCCCCCAATCCTGTGCACACAGAGCCCACTGAGCCTGTGACCTGTCCTTGGCCCCCATCAAGCTCCTTTCCCTATCTAGGGTCCAGGTGAACTTGGCATACTTCCTCCCTCTGAAAACTCAAGAGGTGCCACTCCCCATTCAGGGCACAGGAAGAGCAAGAATTGAACACCGGGATGCAGCATGTGTCCCTGTTGCCACAGGTCTCACCAGAGGCCTCCTTCGTTCTACGACATCGGGCCCTGCTACCTGCAGCGCCCAGGGTGCGCGTCCTCACCTGTGCTGCGGGTGGAACACTCCTCCCTGGCCACTTTCCAGGCCACTGGTTTGCTCTCCGCAAAGGACAAATCCCAGCACCACCTGGTTATTTTGTTCCCTGCCGGCCTCACCTCCTAGAACCCGGGCCACAAGTGCAGGGACCATAGTGTCTGGTTTCGCCATCTCCCTGGCAGGACACATGCTGCACTGAAACACCCAGGGGCTGTGTGGGGAGCAGTGCAGGGGCTGAACAGCGGCCCCCAAATACATGTCTCCTGGGAGTTCCTGATGTGGCCTTATTTGGAAATAGGAGGTCTGCAGATGTCGCTGAGTTAGGACATAGAAGTCACACCATGGGGAGGCCACGAGAAGACAGGCAGAGTGAGTGGAGTGAGGTGACCACCAGCCCAAGATGCCTGGTTCCCCAGGAGCTGGAGAGGGCAGGAAGGACCCTCCCCTTGGGCCTCTGGCGGGAGTGCTGGATCTTGGACTCTGGCCTCCAGAACTGGGAGAATCAGCCTATGTTGAGAGCTGCCCAGTGTGTGGCCCTTCTGCCTGGAAGGGGTGGCTGGGCCTGGCTGTGCATCACTGTGGACTGTCCCCTCTCCCTTGGCCTCTGACATCAGTCACAAAACCCATCCCCTCTCCTTCCTAAATACTTCTGTGGACTGGCAGTGTTGCTGGAAGTCATCTTGGGTGGGGCAGGGACATGGACAGTAAGAGCGGAAGGTGACCCCACATCCTTGCACCTCAAGCTGGTCTCACAATGACAATATGCGTGGAGCGGAGGAGGACTCAGGAGGTGTCTCTGACACCAAACATGGTCATGGCTGGACATGGGGTCAGAACCAGAAGTGAGGAGGCTCCTCAGTGGCCACAGAGGAAGCACCCGGGTGACTTGGGGAGTGGGACAAGGAAGTGGGTCCTCAGGGAAGCAAATGACAAGGGGACAGGAGGGGCTGTTTGCGGATTTAATGGCAGGGCATTGAGGTTGGGAGGGGTCCCAGCTGCTGCGTCTGCTTTTCTTATAGCAGAGAGGCTGCCTACGGGTGGCACGGGGTGGCCATGGAGTGCAGAGTTGGTGGGACAGGGGACATCCAGGGGGCTCGAGATGTTGCTGGTGACAAGGAATGTCAAGTGGCACTGAGGCTGGCGGGCAAGGCCACAGGCAGATTCTCTCACGTGGGTGCTCGCCCCTTTCCTCCCCCTTGTCCCTCCCTCCCACCCTGGCCCCACTCAGGAGTGAGACCCAGTGGCCAATAAGCTCTGGGACAGACGAATGGGCGCCCTCCTCCTTCCTTCTGTTGGGCTGGAGTGAGTGGAGGAGGTGACTCAGCTCCTGGGCTCAGGCAGGGTCTGGAGGGGCCAGGATGGTCTCGAGTGCTTGGCAGCTGAGGAATGTAGCTGGGCTCGGGTAGTAGCAGCAGCGAGGGGCGGGCCAGGGTGGCCAGAGCCCGGGGCCAGGAATGTGCAGCTGAGGTCAATGGTCCCGGAGTCCTCCCGACTCGGGGTCGGGCGGCGGGAAGGAGGGTGGCCGTGGCGGGGGTGGAGGTGGGTGCCCAGGGCTCAGAGCTTGTGGGGGTTCACCCACTTGTAGGTGCCCTCATACTGGAAACCCACTCTCTTCATCAGCTCGTCTGCCTCCGTGGGGCCTCGGCTGGAGAGTGACGGGTGGAGGAGAGGCATGAGGTAGCTCCACCCTCACCCCGCCCCTGCCCGCTGGGCTCTGTCCCCAGCCCCCACCCTTTCCTCACCTGCCATAAATATAGGGGATGGGCTTGGGCTTCTCCAGCTCAATCTGGTGCAGCAGTGGGGTGAAAATACGCCAGGCCTCACGGAGCTCGTCGCTGAGGGGACATAGTATGGCTTGGGAGGCCGGTGGCACACAGGGAGGGAGGGCAAAGGCCACCCCATAGCCCACAGGTATGCAGGGGCCGGCAGCTGGGCCTCACCTGCGCACGAAGTGCATCTGGCTCCCGCAGAAGACGTCCAGGATGAGGCGCTCGTAGGCGTCAGGGAGCTTCACGTTCTGTGAGGGAGAGAGTGTCTTGCTGATGCCACTGCCTGCCACCATGTGGAGTCCCCCGGGCCCAGGCCGCCCACCCTCCACACTGCTCCTTCTCTGTAGGGCACCTTGTATCTGTTGCCGTAGGTCAGGTCCAGCTCCGACTCCTCGGGGTTGAAGAACATGCCCGGCTTCTTGGTCATCATCTTGGTGTACACGGCCTCGTTGGGCTGCACGCGGATCACCAGCTCGTTGCGCTTGCACTGCTGGTGGAAGATGTCGCCGGCCACATCATGGAACTGCAGCCTCACCTCGGCCTTGCGCTCGTTCAGGGCCTTGCCGCAGCGCAGGATGAAGGGCACCCCTACGTGGCGGAAAGGGCAGCCTCAGCACCAGCTCTCTCAGGGTGTGGACCAGTGCGTGAGTGTCTCAGTGGGAGCTCCAGTGCCCGCACACAGGGCATGCCCAGTTCTGCCTTGCTGGGCCTCGAAGGCATCACCTACCATCCCACCTCTCATTCTCCACATAGAGGACGACGGCTGCAAAAGTGGCGGTGGTGGACCCGCGGGGCACCGTGGGGTCGTCCAGGTACCCTTTGGTGGCCTCGCCCTCTCCATCGGGGTTCCCCACGTACTGGCCCAGGACCACATTGTTGGCCTGCACCTCTGAGATGCATTTCAACACCTTGACCTGAGAGAAAGCCAAGGGAGAGAATGGGCTCCTTGGGTGTTGAGTTGGGGTGCAGGGATGACTGTGGCCACAGATGTGCAGCCCTCAGGGCAGGAGGAGGCCCCTGCTTGGCTCTGCTCACCCTGCCAGAGGCCCAGCTCAGGGCCCCTCCCTGAGGACCCTCCAGGACCACCCTGGTCCATCTCGAGTCTATTCTGATGAACAAGCTGAGGCCCAGAGAGGCAATGGCCTTCCCTGGTCACATAGTGACTGTCAGGCCTGGGACATGACAACTTGGGCTTCATGACTGCCAGTCCAGGTCACCTCCGGGAGGCCACGCTGTGCTCAGAGGTGGTGACTTCTCCGGGGTTGAGGACACCTGCTCTGCATGCACACCCCAGCTCAGTGCCTCGTCACAGATGGGCCTGCGACAGGGCATGCTCCTGGGGACTGGGGTGCACCCCCTACCTTCTCATCACGGACGTCATCTGAGTTGGTGGAGGCGGGCTTCTCCATGGCCACCAGACACAGCATCTGCAGTAGGTGGTTCTGCATCACGTCCCTGGGGACGGAAGAGGCCAGAGCTCGCCTTAGCTCCCCGCCCTGTTCACCTGGTTCAAGGGCATGGGGACCCCAAACAAGGCTTCCTAGTGACAAGCTGCAAGACTCACTTCCTGATCCCCCTTGTCTTCCAGGTCCCCTTAAATCAAGGAAGGACATGGTGATGCTATCACTGAATCATAAAACCGTGGAGTGCTTGGCTGTGTAGGGGTCCAGCCCTTTCCAGCCCGGTCTGATAGCTCAGACACTTAGGTTTTGAACTGCAGGGTGAGGAGGAGCTCCCCCAAGATAGGGAAGAGTAGCCCTGCAGGGTGACTGGCTCTGCCACCCTGTGCCAGCCTCCCAGGAGAGAGGAAGAGCTCTCACCGGATGATCCCAAATTCATCGAAATAGCCCCCGCGACCCTCAGTGCCAAAGGGCTCCTTGAAGGTGAGGATAACGCAGGCGATGTTGTCCCGGTTCCAGATGGGGCCGAAGATCCTGTTGGCAAATCTGCAGGGAGGGGCAAGGTGGAGGAACTGACCTTGGGCCTCTGTGGTGCAGGGGCCACATGTGAGGGGTCACCCTTGTCTGAGTTCTGGAGGAATTCGTCCTCGGGGAGGCAGTGGGCCAGGTGAGGCTCCTGAGTACCACCCCCACCCTGGTCCCCCGGCCCAGGCTTGGCCCCACCTCAGCACCATGAGGTTCTGCACCATCTCCTTGCCCAGGTAGTGGTCGATGCGGTAGATCTGGTCCTCACGGAACAGGGAGGAGATGTGGTTGGACAGCCGGTCAGAGCTCTGCAGGTCCCTCCCGAAGGGCTTCTCCACGATGATGCGGTTCCAGCCTCTGCTGGGAGCCCGGAGCTGCGTTACCCCCTTGAACCCCTCTTCGGGGAGTGAGGATCACAGCTGCATCATTCAGACGCCCTCCCAGGGGAGTAGAGGCCAGAACCTCCTCGCCCCCGTGGCCACCTCCCGTGCCTTGTGTTCCCAGATGACCCTCTGGCTCAACACCTTCCGGGAAGCCTTCCCACCCTGGGTGCCAGGGTGGCATTGCTGGCATGCTGCCGGGGGCCCGTTCCCCTCCTGCCTCATGCTGGGTCCCCGGTGGGTCTGAGTGGCCTGAAGGCCTGTAGGGGAGCAGGGGGAGGAGGCATCCAAGCCATGGCTTCCTCATTGGTTTTGAAAATGAGAAGAGGACCTTTGCTTTACTACCCCCGCATTCAAAACCAGCCAGAGGACAAGAGCCTCTGGCTGGGTTGGGAAACCACCCAGCGCGGCCATGCTGCATTCGCAGAGCAAGGCTGCCACCCTGCGGCCTGGCCGGGCCTTTGGGGAAGCAGAGCGGAAAGGCGGTGTTTCGTGGAGCAACGCTGCCACCTTGTGGTCCCGCTGGGGATGGCCCCGGCACCATGGATGCTGCCCAGATCCCCGGCCCCGGACACGCTCATAGAGTGGTGGGAGCACTGCCTGGGCCAGCCTGGCAGGCGGGAAGGGAGGGCAACGGCAAGCCTTACATCTGGCTCATGCAGGACTCGTGAATGTTCTTGGTGACGGCCTCGTAGACGGTCGGGGGCAAGGCCAGGTAGAAGAGGCGGTTGGCCTGTGACCCCAGGTGGAGGGCATCCATGTGGCTGTTGAGGCGCTGGTAGGAGGCTGCATCATCGTACTGGCCAGCCACATAGGAGTTGCGGGCAAAGAAGTCCTCCAGCTTGAGCTTCTCCTCTGGGGTGGCCTGGGAGACACGGACAGACAGACACACAGACAGATGTCAGCCCCTCTCTTTGAGTCCGTGTGTGTTCTGCCCCAGGGGAGTGGAGGGTCTTCCCTGGAGGTCCAGGGAGGGTGCCCTCAGAAGTCAGTGTCCCCGTCCCACACTGGGTTCAGCCCCATCTTAGCAGCTCTGCACATCCAGAGGGAGGGAGGCCAAAGCAGGCAGCACAGACACTGCCCCAGGCTGGGACCCCTGTGCCTTAGGACAGAGGCCAGATTTCAGGATATTTTGACCTGGGAGAAATACACTGGAGAAAGCTCTCTCTCCAAAATCATGACACCCAACTATGATTGGCGGAGAAAACGCAGCAGAGCACAGCAGGAGGGACCTGTGGGTCCTGGTCACGGGGGCTGGTAATGGGGGTCTCAAGAAGTACGAGAGCAGGCGGGGCGGGGCAGGAGAGGAGGAGAGCATCCCGGGATGGGATGGGGGGAGGTCCCCGAAGCTGGCCATGCTGGGGGCTGGTAGAGAGGGCAGAACCAGGCTGGGGGAGGCCCTGACACCACCCACCTTGAAGAAGGGCTCACTCTGTTTGCGGATGTCAGCCACTGTGAGGCGGGAACGGGCATAGCCCATGATGAAGGTGTTTTCGGGCAGAAGGCCATCCCGGAACAGCCACCTGAGGGCAGGGCACAGCTGTAACCAGTGCGGGCAGGGCAGGACCAGGCCTGTCCCTGGCGGGAGGTCACAGGGGCAGTGGTGGGACACACTTACCAGATGGTGGGGTAGATCTTCTTCTTGGCCAGGTCACCCTGTGGCAGAGGGAACAGGTGTGTGGTTAGAAGTGGCTGGGGACACGACCTACATACATCATCCTCCACCCTTGGTGATCTGGGCACTACTCAGGATCACTACTGGGCCACAAGCATGTCTGTCTTGCCTGATACACAGACAGAAGAGCCCCGAGATTCAAGCCTGTGCCAGCCCTCCATCCCTGACCCAGAAGACATAAACGCATCTCCTGCGTTCCAGGAGAAAACCTGAAAATTCAATTCAGTAGAACCAAGGGACGACAGAAGTATCATGTAGCCACATTTGTGAGACGTGACCTCAAGTGCTTACATTAGCAAAAAAGAGTGGTTCAAAAATAAGAAGCTAAGCATTCCACTTAAGAAGTGAGAAAAAGGGCCAGATGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAAGTCAGGAGTTCAAGACCAGCCAATGTGGCAAAACCCCATCTCTATTAAAAATACAAAAATTAGCCAGGTGTGGTGGCAGGCACCTGTAATCCCAGTTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGTGACAAGAGTGAGACTTCATCTCCAAAAAAAAAGTGAGAAAAAGATAGAACTGCAAGCCAAATTACCAGGAAGAAAATAGGAAATAATGACAAGTAAAATTAATAAAATTAAAAGCTAACCCAAAGAAAAGATGAACAAAGCTGGAAATTGGTGTTTGAAAAAGACGAATAGAAACAGACAACCCCCTGGCATGGTTGCTTTTTTAAAAGGGCATAGGCCGGGCACAGTGGCTTACTCCTGTAATCCCAGCACTTTGAGAGGCCGAGGCAGGCGGATCAAGAGGTCAGGAGATCGAGACCATCCTGGCTGACACGGTGAAACCCCGCCTCTACCAAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGTGTCTGTAGTCCCAGCTACTCGGAAGGCTGAGGTAGGAGAATCACTTGAACCCGGGAGATGGAGGTTGCAGTGAGCCAAGATTGCACCATTGCACCCCAGCCTGGGCGACAAGAGTGAAACTCCGTCTCAAAAAACAAAAAACAAAAAAACAACTAAACAGCTGGACGCGGTGGCTCAAGCCTATAATCCCAGCACTTTGGAAGGCCGAGGCGGATGGATTACCTGAGTTCGGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCGTTTCTACTAAAAATACAAAATTGGCTGGGCATGGTGGCCCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATCAAGCCACTGCACTCCAGCCTGGGCTACAAAAGCGAAACTCCGTCTCAAACAAACAAAAAACAGAGCAATGGACCTGAGAGGGGACAGTGGCCACAAATCTTCCTACAGAAACAACCCCCTGGCATAGAGAGTGTTGCCAGTGGGTTCTACCAAAATGCAAGCACAAGAGAATTCCAGCCTGAAGCAAACTCTTCCTGCAGAAGGAAGAAAGGGGAACACTTCCCAAGCCACCTTATGGGGCCTACAGAGCCTTGGTACGGAAACCTGATGAGAAAGGCACATGGGAAAACCCATACGCCTCATTCACACACACATGCAGATGCAGAAATCCCACACGAAATATCAGCAGGCCGGGCACAACTCACACACCCCAGCACTTTGGGAGACCAAGGTGGGAGGAAGATTGCTTGAAATCAGGAGGTTTTTTTTTTTTTTTTTTTTTAAAGACATGGTCTCACTCTGTCACCTAGGCTGGAGTGCAGTGGCACGATCTCAGTTCACTGCAACCTCTGCCTCTGGGCTCAAGCAATCCTCCCACCTCAGCCTCTGGAGTAGCTGGGACTACAGGTACATGCCAACACACCCAGCTAATTTTTTGTATTTTTTGTAGAGACAGGGTTTCCCCATGTTGTCCAGGCTGCTCTTGAACTCCTGGGCTCAAGTGATCTGCCCGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACGACGACCCACTGACCCTGTCTCTTTAAAAAAAAAGAAGAAAACATCAGCAAACCAAATCCTGCAATGTTAAAAACGGTAGGTTTTTTATTTACAGAATAAATACATCTGTAAATAAAAAGTTGGTTTGATATTAACAGAAAAAAATCATTGAAATTCACCATATTAAGAGATTAAAAAAAACCTCTAAGAACTCTCTGCCTACTGCTATGTTTTGATTTTTTAAATCTAGCAATCTTGCTCAACCCAACAGATGTAGATCAAGTGTTTCAGAAATCACCTATTCACGCTAAAACTCTTAGCAGAGGGGGAATGCAAGGGAACTTCACTAACTTGATAAAGGGCATCTATAAAACACCATTCTGGCTGGGCGTGGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGGAATCGCTTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATGGTGAAACCCCATTTCTACTAAAAATACAAAACTTAGCTGAACATGGTGGTGTATGCCTGTAATCCCAGCTATTCAGGAGACTGAGGCAGGAGAATCGCTTGAACCAGGAGGGAGAGACTGGAGTGAGCCGAGATCACACCACTGTAGCCTGAGCAACAGAGCTAGACGTCGTCTCAAAAAAAAAAACCACCACCAAAAAACAAAGAACAAAAAAACCCCCAAACCATCATCCTTACAAATACTACAAATATCACACTTATAGGAAATGCTGAAATATTAATCATCCCTTTAAGCAATCAGGAACAAGAAAAGTGCAGTAAGGCAAGAAAATGAAAATGGCTGAAGTTGAGCTGTGAGGTGGTGCGGGTGCTCCCTCTGATCAAGTGAAACACACACCTATCCTGTGACCTGGCCGTTCCACCCCAAGAGAAATGAAAGTGCAAGTCCACACAAAGACCTGCACACGAATGGTCACAGCAGCCTTGGTTCATAGCGGCCCCAAACTGGAAACAAGTCAAACACTTGAATTAACATGTGACCCGGCAAACTGTGCATTCATACAACGGATACTACTCAGCAACACTGGGAAATCTCAGCATCAGAGATGCAACCCATTGTGACAGAAATCAGATCAGCGGTTGCCTGGAGCAGGGCAGATTGGCTGGGAATGGGTACCCAGGAACTTTCTGGGGAGATGACGATGGCCTTTATTGTGATGAGGGTGTACAGCCAATTGTACAGTTAAGATTTGCGCACCAACAAAAGACGGCGAGAAAACCAACAATGGAGCGGAGGGTGGGGAGTGGGTCAGAAATGACCCTTTGAGGGCCAGTGACTGTGAGAGCCCCAGGTGAGGGGGCTTCCTGGGTGCAGAGCATATTCTGTTTCTTGATCTAGGAGCAGTTTCTGTGGCTGTGCTGAGTTTGTGAAAAGTCATCAACAAGCTAGACCTGCACCATCTGTGTTCTTCATGACATGTATTTCTTTTTTTTTTTGAGACGGAGTCTCACTCTGTCGGCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGAAATCTCCACCTCCTGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTCCAGGCACCTGCCACCACGCCCGGCTAATTTTTGTATTTTTACTAGAGATGGGGTTTCGCCATGTTGGCCAGGCTGGCCTCAAACTCCTGACCTTGTGATCCACCTGCCGTGGCCTCCCAAAGTGCTGGCATTACAGGCGTGAGCCACCGTGGCCGGCCCATGACATATATTTCAATAAAACATGGTAAAAACTAATAGTCTGGGCGTGGTGGCTCATGCCTGTAACCCCAGCACTTTGGGAGGCTGAGGAGGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCAAACATGGTGAAACCCCGTCTCTACTAAAAATACAGGCCAGGCGCGGTGGCTCATGCCTGTAATCCTAGCACTTTGGGAGGTTGAGGTGGGTGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCCAACATGGTGAGACCCCGTTTCTACTAAAATACAAAAAATTAGCTGGATGTGGTGGTGCGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGGGAATTGCTTGAACCTCGCAGGTGGAGATTGCAGTGAGCCAAGATTGCGCCACTGTACTCCAGCCTGGTGAAAGAGCAAGACTCCATCTCAAAAAAAAGAAAAAACAAAAATTAGCTGGATGTGGTGGCAGGCACCTGGAATCCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCACTTGAATCCAGGAGACGGAGGTTGCAGTGAGCCAAGATTGCGCCACCAAACTCCAGCCTGGGTGACAGCAAGACTCCATGTCACGAAAAAAAAAAAAAAAAAACACATGCTCTTCGAAGCAATCAGGCACTGCAACCATGGATGGGTCCATTTCTACAAACCATTACAGAATGGGGGCCATGCCACCAGGTCAAACGCCCTCACCCAGAGCCAGGCAGTTAGGAAGCCACCACAGGGCTTCCAGGAGGGCAACTGCTATGGTGTGGGTGCCTTGTGTGCTTGCGCTCTTCAGGGGCCCTGCTCCACTTCTGATGCTCCTCAGGTGAGCTCGGTGCCAGGACTACAGCGACACGGACCTCTCACTGCTGCACCCTTCAAAGTCCAGCAGACAACCCCTCGCCCTGGGGTAGGAGCTCCTCTACTCAGCTCCTCACAGCACCTGCCATCAGTGGGGCTGGGGAGGGGAACGTTGCCCATGTCACATCAACTCACGGCTGGCCCCACACAGAAAGGGTCAGCTTACTGGGAACTAACTGGACCCTGAAATAAACGGAAAGAACCACAGCCTGCAAATACCTCTAAGCCTCACTCAAAGCAGGTCATGTTTGCTCATGACCAAATTCCGGAAGACTGGGAGGCATCGGGACAGACTCCAGAAAGGTACTGGTGGCAGGGGACTGGTCTGCTGAGTCACAGCAACCCCACGCTCCAGGCCGTTTGTTCCTGCTGCTCCCTTTGGGATTAACTAGAGATTGAACCGGCTCACCTTGTTTCAGCTCAGGCCCTGCAACCGACCGACCGTCTGCTGGGCTCCAAGTCACCTGAGAGGCGAGGTGAGGTAAGACGGACAGTAGGGGTGGGGTAAGGCAGGCCGCTCTCTCCCATGGTGACAGGGAGCTAAGCTGAGCTGAGCCGGACTGAGCTGCTCTCCTCAGGACTCAGGGGTAGCCCTGCCCTCCCAGCTGGCTGCGATGCTCTAGTGGGACTTTCTGGAGGAACAAACATGGGAAGACATCGGGAAACCTGACAGCTTGGTCAAGAGTCTACTCGTGCCATGTGGGTGGGGGCAATTCAAGGGGTCCTTAGTGAAAAGCAGTTGGGAGCTTCTCATCTTCCCCACGCCCTCCTCTTTCCTGAGGACCACAGCAGGGGAGGGATCTGCCCAAGGACACAAGGTGACTTAGTAGAAGCAGGGCATGATGAAGATCTCTAGCCACCAACATCTGGCTAGGCCAGGGCAAAGGGACCTGGGCCATGACCTGTCTGTTTTATAAACCGAAATCGCTGCAGCCCTTGTCTTGGCATGAGGGTTTCATGGTAGAGCACGATGCCTGCTGGAGGGCCAGCGGGGGTGGGCCATCCCATCCCCAAGGGTCACTGGGGTTAAGGACCTGCCCAGACTCCCTGGGTGGAGCCCTGGGCCTTGGCAGGGGCCCAGCTGGTTCCTGGCCACTGGGGCCAGAGAACCTGGGGGCTGCTGAGTTAGTTCAGCTGAAAAACCAACAAAAGGGCCTCCCTGCTATACCCGGGGGCTCATGAGTCACCGGGGCTTTGGGGGAGTGCCAACATCATCATGACACAGCAAGAATCTTATCAGACCAATGGGGAAGTCAGCCCAGAAATGTTCTGAGGAAAGGGGAGGCGGGGGCCGCTGGGTCATCCCTCCCACAGGCCCATCATTGGGATGCGTCCTGAACGCCCATCAAGCCCATGGCCCTTGTGATCCAGGTGGGGAAACTAAGGCCCAGAGAAGTGAGGACCCCGCAGACTATCAATCCCAGTCTCTTCCCCTCACTCCCTGTGAAGCTCTCCAGCATCATCGAGGTCCCATCAGGTGGGGAAAGATGCTGTTCCAGGCGCACACTAGTCTACAAGGCCAGAGCTTTCTGGAAGGGGGCAGTAAGTACCTCGGCTCCCTTTCTGGTAGGGGTGGGAGTCCTGAGAAGGCAGGAAGTGGCCCACTTGGTAACTCTGAGGTGCCATCAGGGCCCCCAGGAAGGAAGCTGGGTGTGTGGGCAAGTGTGAGGTAAGCTGGCCAGGGAGGAGGAAGGGACAGAGGAAGGCCACGTGGGTCCAGCCTGCCCCAGGGTGTCCTGCTTGCCCAGGCTGTGGGTCTGCCAGCCACTTGCCTGCTTTCAGTTTCTAGGTCATGCTGAGCTTGTTCCCAACTCGGGGCTCCGGGCATTTGCTCTTCCTTCTGTGTTTGTGCTCTCCCGGCCCTCTTTGTGGGATCTATGCGCTTTGGGGGACTGGGGACACAGGGCCCATGTGTATCTTCTGAAACACACGTCAGCCTGAACTCTTGCTGGTCTGCTTACTTGCCGTGGTTCCCTGGCCGCAATAGCACATAGGTCCCATGAGGAGGGCAGGGACTGGCTGCTGTGGCCCAGAAGTACCCAATCGCTGTCTGCTCAATGAACGGAGAATGGGCTGCTTTCCTGGAACAGCAGATTCTAGGATCACGTGCCCTCAAGTGCCACCCTGCCTACCTCCCGCACCGAGTGAGGCATCAGGCGTGGAAGAAGCCTGGGAGCCGGAGCTGTTCCAGGTGCTGCCTGAGCACGCCACCTCCCATCTCCCCCACAGCAGGAAGAGGAAAAAACAAACCACGAGGCTCTTCAGAGAGAGGACCCCTTGTCCCCTACCCACAGTGCTGGAGCTGGCACTTCCTATTTCTGCTTTGAAAGCCTCAGGTTGTCACTCTCAGAACAGAGGAGAGCAAAGGGGAACCCTACTGATTTCAACAAAACAAAGTTGCCCAACCCGAAGCTGGCCACAGGCGGAGGCAGTGAAATGACAAAATCAGCTTGGAAAAACCTAAGGACCCTGGGCCCTCGTTTCAAAAGCTGTCATTTGCTACACGAAATGCTGAGGTTCAGGAAAAGGAAGACACTTGCTCCAACTCACACAGCAAGCTTGGATGCTCTCAATGAGGTGTCTAATAAGAGCTGAAGGCCAGGAGTCATAAGCTATCATTGTGGCCTGGGTTGCTCTGTTGCAGTGCCTTGTGACAGCATGGGGTGGGGATGGAGAAAGCAACCTCAGTTACCTTCTCCTCCAGTCTGCTTCTTGGACTAAGGGTTTAACGGTCAGAGTCCTGGCTGTTAAGGTTTGTGGCTGATGCAGGTATGGCTCTTTTTATTTTTATTTTTTTGAGATGGAGTCTCACTCACTCCGTCACCCAGGATGGAGTGCAATGGTGCCATCTCAGCTCACTGCAACCTCCACCTCCTGGGTTCAAGCGATTTTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGATGGGTGCCACTACACCTAACTAATTTTTGTTATTTTTTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCACCCAGGCTGGAATGCAGTGTTGCGATTTCTGCTCACTGCAAGCTCCGCCTCCCGAGTTCACGCCTTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGACTACAGGTGCCCACACCAGGCCTGGCTAATTTTTTATTTAGTAGAGACGGGATTTCACCATGTTAGCCAGGATGGTCTCAATCTCCTGACCTCATGATCCACCCGCTTCAGCCTCCCAAAGTGCTGGGATTACAGACGTGAGCCACTGCGCCCGGCCTTTTTGTTTTGTTTTGTTTTGAGACAGAGTCTCGCTCTGTCGCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAACCTCTGACTCCCTGATTCAAGCGATTCTACTGCCTCAGCCTACTGAGTAGCTGGGACTACAGGCACGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCTTGACCTTGTGATCTGCCCACCTTGGCCCCCCAAAGTGCTGGGATTACAGATGTGAGCCACTGCGCCCAGCCAATTTTTGTATTTTAAGTAGAGACTGGGTTTCGCCATGTTGGCCAGGCTGGTGTCAAACTCCTGACCTCAGGCGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGAATACAGGCGTGAGCCACCGTGCCCGGCCAGGTATGGCTCTTCTGAGGGGACCAGGCTGGGGCTGGGGCTGAGGCCAAGCCCAATCTACTGTGGGCTCCACCTGGTACCTCTCCTGGGTCTCAGGCTTATGGGGAGTCAGAGGACAATGGCCCCTCCTTACTCTGCCACTGGCAGAGCCCTTCTCCCTCGGCTGCCTCCTCATTCCCTTTTGGCTCTCCTTTTCTAAGTTCTGATCAGAAGTACAAAGGTGTCAAGGAGTAGGTTTGACAAAGTGTGACAGCGCGTTGTTCTATGTGAACAAAGAACCACTGAGCTCAGCCAGCACTGAGGGGCGCACGATGTGGAAGAACTAACTAGTTTTGATAGAGCTCCTGCTCAAGGTTACAAGGTAAGTTAATGGCAAAGATGGTCATACAGTAATGAGCAGAGAGATTCTTGAGTGCATCCAGGGTTAAAAGTGAAAACTGAGACCACGTGTAATTTGAGATGAAGCCCTTGTTCCACCAGCCCCTGCACAGTCTCACTGCCCCATGGGACACAGGGGAGGGTGCTCTAGTCTCGGGCGATGGCTGTCCTAGGACCACCCCTCCCTCCCCTCCCATGGAAATCCTCATGCTATACTATTCTTTTGTCTTTCCTATGAGTGCAGAATGGCGGTTCTACAAGCTGGACAATTGGGGCCAGGTGGGGTGAGGCAGAGCTCCTTGGTAGGCTTTCGAAATTGAGGCAAAGAGACAGTGTTCAAAGAAAAGCTAAGTGTTTGTATCGACGGAACTTGAAGTGTTAGTGAAGAGGCAGAGATCAGGCCTCAGATCCTGCCTTTGGAACTCATTTGTTAAGGCATGAACAGGTCTGAAAAAACTACAGAATGAATAGCATTCCCTGTTTTCCCCAAGAAGTCCATCTAGACAGTCCCTAAAGAGCCTGCAACTCCAGGATTAAGGGCTACATTCAGCGGCTAGGCACAGGGTTCAGAAACGTCCTGCAGCCGGGCGTGGTGGCTCATGCCTGTCATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGTCAACATGGTGAAACTCCGTCTCTACTAACAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCTCAGCTACTTGGGGGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCCGAGGTTGCAGTGAGCCAAGATGGCGCCACTGCACCCCATCCTGGGCGACCAGAGCAAAACTCCGTCTCCAAAAAAAAAAAAAAAAAAAAGTCTTGCAAGTGCATATGCACACCAGGTAGAGCCGGGATGATCCTGGCGCACTAGCAGGAGCGGGAGGAGGAGCTCAACTTAGCAGAGCCTGTGGGGCCCTGCAACAATTAGTTGGAAAAGCTGAGGCATGGAGCAGGCACTTCCTGGCTTTTAAGATTGGGGCCTGGGAGATACTCACCGATGCACCCATGATGATGAATATGTGTGTATCCGACTGATGGAAGGCATCGCCCTGGAAAAGCTCTTCCCGCAGGATCCCGCACACCTGGGTCCGGCTCAGGGCCACCTGCTCTGCCATGACGCTGTCTGGTGGAAGAAAGGCTCGTTAACAAGGCAGAAGAACAGGAGAGCATTGAGAAGTTAGCCCCTTTCTTGAGAGTTCCTCTGGGGTCTCACACCAGGGTGACACCTGATTGCCCAAATCACTCCTTGTGAACGGCTGGGCATTGGGGAGTGGTTGATGGGTTAGAAACTGCTGGCTCTGGGCTCCAGCCACTCTCTCCTAGGCAGCCTTCATTCAGGGTGTACATTACAAAATTACTCAAAGCATTGGTGTATTCCGACTACAGCATCAATTCTGGACAACCGAGTAAAATCCTTGTGGGGCATGGAACTGCGTGCCCAGGACCATCTCATTCCCGACTGTAGCGGGAACTCCACAATGACCTGGAGCATGGGAGATGGTGCAGATGCTGGAGTTCAGCTCCAGCCCTCCCCTTGCCAAGCTGGGTGACCCCGGTCGGCAAGTCCCCTTCGCTCTCGGGGTCTCAGGGGCTCAAGAGAGGAGGTGCGGGGTATAAAGGGATTGGTTAAGACCCTCTCGATTCTGCTCGGTTCTCAAGCACAACAAACAGCGTGTATTTTACCGCCGCGCGGCGCAGCGCGGGACAGTACGCTCCTCCGCCTGCGCGGCGCCCGCCCGGCCGGTTACCTGCGCTTCGTCGTCGTCGCCCTCCGCGCTCGCAGCCCCGAAGTGTACGACCGTTTCCGGGGGCTGAGCCCCGCCGGCCCATTTAATCGGCGGGGGCGGGGGCGGGCGCCTGGGCTGAGCGGACCCGCCTCAGGCGAGGCGTGCGGGGCGGGGCCTCGGCCACCACCCCTCGTGCGGGCGGGGCGGGGCGAGGGCAGGTGCGCGCGCATCCCAGGCCAGCCCCTGCCTCTCGGGCACCTGCGCTGGAGGCCGGCCCGCCGGCTGCCTGCCATACCCGCTGCCGCTGCTCTGCATCCCCAATTCCGGCGGGCACGGGTGCAGCTCCGCGTAGTGCTCCCGCATCCCCATCGCCGGCCCGGCCCCGCCCCTACTGTCCGGTTTCCCCGCCTGCCCCGGCGGCCTCGCGCGCTCGCGGAGGGCTCCACTTCCGCCGGCAGCGTGGCCACGCCTCCTCTGGGCTGTCCCTCGGCTCCTGGGCGGGGCCTTCTGCCGCGCCACTCCCCCGGGAACCCTCGCCTGTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCGAGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGACTTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCACAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCCGCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGGTGTGGTCCTTTTCCCCAAAGACAGGGTTCCATCCGTGGGCGTTCCGCCGCCTCCGAAACTTCCCCCGGACGTTCAGGCTCCCCCCTCTTTTTTGGGCCCCAGCCCGTTCCTGCTCCGCGCTTCTGGAGCACTGGCCAAGGCGGGCCGATTCAGGACCCAGGTTACTTGGGCGGCGAGCTGGACTGTTTCTACTCCTCCCTCCTCCTCCACTGCGGGGTCTGACCCTACTCCTTGTGTGAGGACTCCTCTAGTTCAGAGACATATTCTGTTCACCAAACTTGACTGCGCTCTATCGAGGTCGTTAAATTCTTCGGAAATGCCTCACATATAGTTTGGCAGCTAGGTATCTGATTTCATATGCCTGTTTGCTCGTTTTGCAAGACAACATCTGCCTATCGTCATACTGTTTCTGTGATCTGAGAATGAATGGGCTCTCCTGGCACATTAGAGGAATAGCACGGAGGTCACTATAGCTGGAGCAGAGTGAGGGAGGGGAGCACAGTTGGAGAGGAAGGGATGGGGAACCCGATGGTACAGGGCCTTGTAGGCCGTTGTAGGGAGATTGGCTTTTACGCAGAAGGCAACGGGGATCCATGGTAGGGTTCTCAGTAGAGGAGGAATGTGATCGGAATTACGTCTTACACTGATCGTTCTAGCAGTGGTGGGGAGACCAGACCATAGTGGGCAAGGGTAAAAGAAGGGTGGCGTGGTAAGAGGTAATTGCAGTAATCCAGCTCAGAGACAGTGCTTTGAGCATTTTATTATTGGAAATTTCAAAAATGTACGAAAGTAGAAAAATGAGTATAGTAAATCTTTTTGGTTTGTTTGTTTTTGAGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTCTAACCTCCTCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCTTCCCAGGTAGCTGGGACTGCAGGCGTGCGCCACTACGCCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACTATATGTTGGCCAGTCTGGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGACTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCGCTCGGCCAGTATAGTAAATCTTACATTCACAGCTACCAACTTCAACAATTGAAGGTATCAACTTCTGACTAATTTTGTTTATATCCCTCGCAATCCTCTCCCATTATTATTTTATTTCTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTTTTTGAGACAGAATCTCGCTCTGTCACCAGGCTGGAGTGCAGTGGTGTGATCTCCACCCACTGCAACTTCCGCCTCCTGGATTCAAGCGATTCTTCTGCCTCAGCCGCTGGGACTACAGTTGTGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCATCATGTTGGCCAGGATGGTCTTGATCTCTTGACCTTGTCATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCGGCCTCTTTTTTTGAGACCCAGTCTCACTCTGTCGCCCAGAATGGAGTGCAGTGGCACGATCTTGGCTCACCGCAACCTCCATCTCCCAGGTTTGAGCGATTCTCCTGCCTCAGCTTCCCAAATAGCTGGGATTACAGGCATGCACCATCACTCCCAGCTATTTTTTTTTTTTTTGTATTTTTGGTAAATACAGGGTTTTGTCATGTTGGCCAGGCTGGTCTTGAATTC PRIMER_OPT_SIZE=27 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=30 = 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CAGCGAGGGGCGGGCCAGGGTGGCCAGAGCCCGGGGCCAGGAATGTGCAGCTGAGGTCAATGGTCCCGGAGTCCTCCCGACTCGGGGTCGGGCGGCGGGAAGGAGGGTGGCCGTGGCGGGGGTGGAGGTGGGTGCCCAGGGCTCAGAGCTTGTGGGGGTTCACCCACTTGTAGGTGCCCTCATACTGGAAACCCACTCTCTTCATCAGCTCGTCTGCCTCCGTGGGGCCTCGGCTGGAGAGTGACGGGTGGAGGAGAGGCATGAGGTAGCTCCACCCTCACCCCGCCCCTGCCCGCTGGGCTCTGTCCCCAGCCCCCACCCTTTCCTCACCTGCCATAAATATAGGGGATGGGCTTGGGCTTCTCCAGCTCAATCTGGTGCAGCAGTGGGGTGAAAATACGCCAGGCCTCACGGAGCTCGTCGCTGAGGGGACATAGTATGGCTTGGGAGGCCGGTGGCACACAGGGAGGGAGGGCAAAGGCCACCCCATAGCCCACAGGTATGCAGGGGCCGGCAGCTGGGCCTCACCTGCGCACGAAGTGCATCTGGCTCCCGCAGAAGACGTCCAGGATGAGGCGCTCGTAGGCGTCAGGGAGCTTCACGTTCTGTGAGGGAGAGAGTGTCTTGCTGATGCCACTGCCTGCCACCATGTGGAGTCCCCCGGGCCCAGGCCGCCCACCCTCCACACTGCTCCTTCTCTGTAGGGCACCTTGTATCTGTTGCCGTAGGTCAGGTCCAGCTCCGACTCCTCGGGGTTGAAGAACATGCCCGGCTTCTTGGTCATCATCTTGGTGTACACGGCCTCGTTGGGCTGCACGCGGATCACCAGCTCGTTGCGCTTGCACTGCTGGTGGAAGATGTCGCCGGCCACATCATGGAACTGCAGCCTCACCTCGGCCTTGCGCTCGTTCAGGGCCTTGCCGCAGCGCAGGATGAAGGGCACCCCTACGTGGCGGAAAGGGCAGCCTCAGCACCAGCTCTCTCAGGGTGTGGACCAGTGCGTGAGTGTCTCAGTGGGAGCTCCAGTGCCCGCACACAGGGCATGCCCAGTTCTGCCTTGCTGGGCCTCGAAGGCATCACCTACCATCCCACCTCTCATTCTCCACATAGAGGACGACGGCTGCAAAAGTGGCGGTGGTGGACCCGCGGGGCACCGTGGGGTCGTCCAGGTACCCTTTGGTGGCCTCGCCCTCTCCATCGGGGTTCCCCACGTACTGGCCCAGGACCACATTGTTGGCCTGCACCTCTGAGATGCATTTCAACACCTTGACCTGAGAGAAAGCCAAGGGAGAGAATGGGCTCCTTGGGTGTTGAGTTGGGGTGCAGGGATGACTGTGGCCACAGATGTGCAGCCCTCAGGGCAGGAGGAGGCCCCTGCTTGGCTCTGCTCACCCTGCCAGAGGCCCAGCTCAGGGCCCCTCCCTGAGGACCCTCCAGGACCACCCTGGTCCATCTCGAGTCTATTCTGATGAACAAGCTGAGGCCCAGAGAGGCAATGGCCTTCCCTGGTCACATAGTGACTGTCAGGCCTGGGACATGACAACTTGGGCTTCATGACTGCCAGTCCAGGTCACCTCCGGGAGGCCACGCTGTGCTCAGAGGTGGTGACTTCTCCGGGGTTGAGGACACCTGCTCTGCATGCACACCCCAGCTCAGTGCCTCGTCACAGATGGGCCTGCGACAGGGCATGCTCCTGGGGACTGGGGTGCACCCCCTACCTTCTCATCACGGACGTCATCTGAGTTGGTGGAGGCGGGCTTCTCCATGGCCACCAGACACAGCATCTGCAGTAGGTGGTTCTGCATCACGTCCCTGGGGACGGAAGAGGCCAGAGCTCGCCTTAGCTCCCCGCCCTGTTCACCTGGTTCAAGGGCATGGGGACCCCAAACAAGGCTTCCTAGTGACAAGCTGCAAGACTCACTTCCTGATCCCCCTTGTCTTCCAGGTCCCCTTAAATCAAGGAAGGACATGGTGATGCTATCACTGAATCATAAAACCGTGGAGTGCTTGGCTGTGTAGGGGTCCAGCCCTTTCCAGCCCGGTCTGATAGCTCAGACACTTAGGTTTTGAACTGCAGGGTGAGGAGGAGCTCCCCCAAGATAGGGAAGAGTAGCCCTGCAGGGTGACTGGCTCTGCCACCCTGTGCCAGCCTCCCAGGAGAGAGGAAGAGCTCTCACCGGATGATCCCAAATTCATCGAAATAGCCCCCGCGACCCTCAGTGCCAAAGGGCTCCTTGAAGGTGAGGATAACGCAGGCGATGTTGTCCCGGTTCCAGATGGGGCCGAAGATCCTGTTGGCAAATCTGCAGGGAGGGGCAAGGTGGAGGAACTGACCTTGGGCCTCTGTGGTGCAGGGGCCACATGTGAGGGGTCACCCTTGTCTGAGTTCTGGAGGAATTCGTCCTCGGGGAGGCAGTGGGCCAGGTGAGGCTCCTGAGTACCACCCCCACCCTGGTCCCCCGGCCCAGGCTTGGCCCCACCTCAGCACCATGAGGTTCTGCACCATCTCCTTGCCCAGGTAGTGGTCGATGCGGTAGATCTGGTCCTCACGGAACAGGGAGGAGATGTGGTTGGACAGCCGGTCAGAGCTCTGCAGGTCCCTCCCGAAGGGCTTCTCCACGATGATGCGGTTCCAGCCTCTGCTGGGAGCCCGGAGCTGCGTTACCCCCTTGAACCCCTCTTCGGGGAGTGAGGATCACAGCTGCATCATTCAGACGCCCTCCCAGGGGAGTAGAGGCCAGAACCTCCTCGCCCCCGTGGCCACCTCCCGTGCCTTGTGTTCCCAGATGACCCTCTGGCTCAACACCTTCCGGGAAGCCTTCCCACCCTGGGTGCCAGGGTGGCATTGCTGGCATGCTGCCGGGGGCCCGTTCCCCTCCTGCCTCATGCTGGGTCCCCGGTGGGTCTGAGTGGCCTGAAGGCCTGTAGGGGAGCAGGGGGAGGAGGCATCCAAGCCATGGCTTCCTCATTGGTTTTGAAAATGAGAAGAGGACCTTTGCTTTACTACCCCCGCATTCAAAACCAGCCAGAGGACAAGAGCCTCTGGCTGGGTTGGGAAACCACCCAGCGCGGCCATGCTGCATTCGCAGAGCAAGGCTGCCACCCTGCGGCCTGGCCGGGCCTTTGGGGAAGCAGAGCGGAAAGGCGGTGTTTCGTGGAGCAACGCTGCCACCTTGTGGTCCCGCTGGGGATGGCCCCGGCACCATGGATGCTGCCCAGATCCCCGGCCCCGGACACGCTCATAGAGTGGTGGGAGCACTGCCTGGGCCAGCCTGGCAGGCGGGAAGGGAGGGCAACGGCAAGCCTTACATCTGGCTCATGCAGGACTCGTGAATGTTCTTGGTGACGGCCTCGTAGACGGTCGGGGGCAAGGCCAGGTAGAAGAGGCGGTTGGCCTGTGACCCCAGGTGGAGGGCATCCATGTGGCTGTTGAGGCGCTGGTAGGAGGCTGCATCATCGTACTGGCCAGCCACATAGGAGTTGCGGGCAAAGAAGTCCTCCAGCTTGAGCTTCTCCTCTGGGGTGGCCTGGGAGACACGGACAGACAGACACACAGACAGATGTCAGCCCCTCTCTTTGAGTCCGTGTGTGTTCTGCCCCAGGGGAGTGGAGGGTCTTCCCTGGAGGTCCAGGGAGGGTGCCCTCAGAAGTCAGTGTCCCCGTCCCACACTGGGTTCAGCCCCATCTTAGCAGCTCTGCACATCCAGAGGGAGGGAGGCCAAAGCAGGCAGCACAGACACTGCCCCAGGCTGGGACCCCTGTGCCTTAGGACAGAGGCCAGATTTCAGGATATTTTGACCTGGGAGAAATACACTGGAGAAAGCTCTCTCTCCAAAATCATGACACCCAACTATGATTGGCGGAGAAAACGCAGCAGAGCACAGCAGGAGGGACCTGTGGGTCCTGGTCACGGGGGCTGGTAATGGGGGTCTCAAGAAGTACGAGAGCAGGCGGGGCGGGGCAGGAGAGGAGGAGAGCATCCCGGGATGGGATGGGGGGAGGTCCCCGAAGCTGGCCATGCTGGGGGCTGGTAGAGAGGGCAGAACCAGGCTGGGGGAGGCCCTGACACCACCCACCTTGAAGAAGGGCTCACTCTGTTTGCGGATGTCAGCCACTGTGAGGCGGGAACGGGCATAGCCCATGATGAAGGTGTTTTCGGGCAGAAGGCCATCCCGGAACAGCCACCTGAGGGCAGGGCACAGCTGTAACCAGTGCGGGCAGGGCAGGACCAGGCCTGTCCCTGGCGGGAGGTCACAGGGGCAGTGGTGGGACACACTTACCAGATGGTGGGGTAGATCTTCTTCTTGGCCAGGTCACCCTGTGGCAGAGGGAACAGGTGTGTGGTTAGAAGTGGCTGGGGACACGACCTACATACATCATCCTCCACCCTTGGTGATCTGGGCACTACTCAGGATCACTACTGGGCCACAAGCATGTCTGTCTTGCCTGATACACAGACAGAAGAGCCCCGAGATTCAAGCCTGTGCCAGCCCTCCATCCCTGACCCAGAAGACATAAACGCATCTCCTGCGTTCCAGGAGAAAACCTGAAAATTCAATTCAGTAGAACCAAGGGACGACAGAAGTATCATGTAGCCACATTTGTGAGACGTGACCTCAAGTGCTTACATTAGCAAAAAAGAGTGGTTCAAAAATAAGAAGCTAAGCATTCCACTTAAGAAGTGAGAAAAAGGGCCAGATGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAAGTCAGGAGTTCAAGACCAGCCAATGTGGCAAAACCCCATCTCTATTAAAAATACAAAAATTAGCCAGGTGTGGTGGCAGGCACCTGTAATCCCAGTTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGTGACAAGAGTGAGACTTCATCTCCAAAAAAAAAGTGAGAAAAAGATAGAACTGCAAGCCAAATTACCAGGAAGAAAATAGGAAATAATGACAAGTAAAATTAATAAAATTAAAAGCTAACCCAAAGAAAAGATGAACAAAGCTGGAAATTGGTGTTTGAAAAAGACGAATAGAAACAGACAACCCCCTGGCATGGTTGCTTTTTTAAAAGGGCATAGGCCGGGCACAGTGGCTTACTCCTGTAATCCCAGCACTTTGAGAGGCCGAGGCAGGCGGATCAAGAGGTCAGGAGATCGAGACCATCCTGGCTGACACGGTGAAACCCCGCCTCTACCAAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGTGTCTGTAGTCCCAGCTACTCGGAAGGCTGAGGTAGGAGAATCACTTGAACCCGGGAGATGGAGGTTGCAGTGAGCCAAGATTGCACCATTGCACCCCAGCCTGGGCGACAAGAGTGAAACTCCGTCTCAAAAAACAAAAAACAAAAAAACAACTAAACAGCTGGACGCGGTGGCTCAAGCCTATAATCCCAGCACTTTGGAAGGCCGAGGCGGATGGATTACCTGAGTTCGGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCGTTTCTACTAAAAATACAAAATTGGCTGGGCATGGTGGCCCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATCAAGCCACTGCACTCCAGCCTGGGCTACAAAAGCGAAACTCCGTCTCAAACAAACAAAAAACAGAGCAATGGACCTGAGAGGGGACAGTGGCCACAAATCTTCCTACAGAAACAACCCCCTGGCATAGAGAGTGTTGCCAGTGGGTTCTACCAAAATGCAAGCACAAGAGAATTCCAGCCTGAAGCAAACTCTTCCTGCAGAAGGAAGAAAGGGGAACACTTCCCAAGCCACCTTATGGGGCCTACAGAGCCTTGGTACGGAAACCTGATGAGAAAGGCACATGGGAAAACCCATACGCCTCATTCACACACACATGCAGATGCAGAAATCCCACACGAAATATCAGCAGGCCGGGCACAACTCACACACCCCAGCACTTTGGGAGACCAAGGTGGGAGGAAGATTGCTTGAAATCAGGAGGTTTTTTTTTTTTTTTTTTTTTAAAGACATGGTCTCACTCTGTCACCTAGGCTGGAGTGCAGTGGCACGATCTCAGTTCACTGCAACCTCTGCCTCTGGGCTCAAGCAATCCTCCCACCTCAGCCTCTGGAGTAGCTGGGACTACAGGTACATGCCAACACACCCAGCTAATTTTTTGTATTTTTTGTAGAGACAGGGTTTCCCCATGTTGTCCAGGCTGCTCTTGAACTCCTGGGCTCAAGTGATCTGCCCGCCTCAGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACGACGACCCACTGACCCTGTCTCTTTAAAAAAAAAGAAGAAAACATCAGCAAACCAAATCCTGCAATGTTAAAAACGGTAGGTTTTTTATTTACAGAATAAATACATCTGTAAATAAAAAGTTGGTTTGATATTAACAGAAAAAAATCATTGAAATTCACCATATTAAGAGATTAAAAAAAACCTCTAAGAACTCTCTGCCTACTGCTATGTTTTGATTTTTTAAATCTAGCAATCTTGCTCAACCCAACAGATGTAGATCAAGTGTTTCAGAAATCACCTATTCACGCTAAAACTCTTAGCAGAGGGGGAATGCAAGGGAACTTCACTAACTTGATAAAGGGCATCTATAAAACACCATTCTGGCTGGGCGTGGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGGAATCGCTTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATGGTGAAACCCCATTTCTACTAAAAATACAAAACTTAGCTGAACATGGTGGTGTATGCCTGTAATCCCAGCTATTCAGGAGACTGAGGCAGGAGAATCGCTTGAACCAGGAGGGAGAGACTGGAGTGAGCCGAGATCACACCACTGTAGCCTGAGCAACAGAGCTAGACGTCGTCTCAAAAAAAAAAACCACCACCAAAAAACAAAGAACAAAAAAACCCCCAAACCATCATCCTTACAAATACTACAAATATCACACTTATAGGAAATGCTGAAATATTAATCATCCCTTTAAGCAATCAGGAACAAGAAAAGTGCAGTAAGGCAAGAAAATGAAAATGGCTGAAGTTGAGCTGTGAGGTGGTGCGGGTGCTCCCTCTGATCAAGTGAAACACACACCTATCCTGTGACCTGGCCGTTCCACCCCAAGAGAAATGAAAGTGCAAGTCCACACAAAGACCTGCACACGAATGGTCACAGCAGCCTTGGTTCATAGCGGCCCCAAACTGGAAACAAGTCAAACACTTGAATTAACATGTGACCCGGCAAACTGTGCATTCATACAACGGATACTACTCAGCAACACTGGGAAATCTCAGCATCAGAGATGCAACCCATTGTGACAGAAATCAGATCAGCGGTTGCCTGGAGCAGGGCAGATTGGCTGGGAATGGGTACCCAGGAACTTTCTGGGGAGATGACGATGGCCTTTATTGTGATGAGGGTGTACAGCCAATTGTACAGTTAAGATTTGCGCACCAACAAAAGACGGCGAGAAAACCAACAATGGAGCGGAGGGTGGGGAGTGGGTCAGAAATGACCCTTTGAGGGCCAGTGACTGTGAGAGCCCCAGGTGAGGGGGCTTCCTGGGTGCAGAGCATATTCTGTTTCTTGATCTAGGAGCAGTTTCTGTGGCTGTGCTGAGTTTGTGAAAAGTCATCAACAAGCTAGACCTGCACCATCTGTGTTCTTCATGACATGTATTTCTTTTTTTTTTTGAGACGGAGTCTCACTCTGTCGGCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGAAATCTCCACCTCCTGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTCCAGGCACCTGCCACCACGCCCGGCTAATTTTTGTATTTTTACTAGAGATGGGGTTTCGCCATGTTGGCCAGGCTGGCCTCAAACTCCTGACCTTGTGATCCACCTGCCGTGGCCTCCCAAAGTGCTGGCATTACAGGCGTGAGCCACCGTGGCCGGCCCATGACATATATTTCAATAAAACATGGTAAAAACTAATAGTCTGGGCGTGGTGGCTCATGCCTGTAACCCCAGCACTTTGGGAGGCTGAGGAGGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCAAACATGGTGAAACCCCGTCTCTACTAAAAATACAGGCCAGGCGCGGTGGCTCATGCCTGTAATCCTAGCACTTTGGGAGGTTGAGGTGGGTGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCCAACATGGTGAGACCCCGTTTCTACTAAAATACAAAAAATTAGCTGGATGTGGTGGTGCGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGGGAATTGCTTGAACCTCGCAGGTGGAGATTGCAGTGAGCCAAGATTGCGCCACTGTACTCCAGCCTGGTGAAAGAGCAAGACTCCATCTCAAAAAAAAGAAAAAACAAAAATTAGCTGGATGTGGTGGCAGGCACCTGGAATCCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCACTTGAATCCAGGAGACGGAGGTTGCAGTGAGCCAAGATTGCGCCACCAAACTCCAGCCTGGGTGACAGCAAGACTCCATGTCACGAAAAAAAAAAAAAAAAAACACATGCTCTTCGAAGCAATCAGGCACTGCAACCATGGATGGGTCCATTTCTACAAACCATTACAGAATGGGGGCCATGCCACCAGGTCAAACGCCCTCACCCAGAGCCAGGCAGTTAGGAAGCCACCACAGGGCTTCCAGGAGGGCAACTGCTATGGTGTGGGTGCCTTGTGTGCTTGCGCTCTTCAGGGGCCCTGCTCCACTTCTGATGCTCCTCAGGTGAGCTCGGTGCCAGGACTACAGCGACACGGACCTCTCACTGCTGCACCCTTCAAAGTCCAGCAGACAACCCCTCGCCCTGGGGTAGGAGCTCCTCTACTCAGCTCCTCACAGCACCTGCCATCAGTGGGGCTGGGGAGGGGAACGTTGCCCATGTCACATCAACTCACGGCTGGCCCCACACAGAAAGGGTCAGCTTACTGGGAACTAACTGGACCCTGAAATAAACGGAAAGAACCACAGCCTGCAAATACCTCTAAGCCTCACTCAAAGCAGGTCATGTTTGCTCATGACCAAATTCCGGAAGACTGGGAGGCATCGGGACAGACTCCAGAAAGGTACTGGTGGCAGGGGACTGGTCTGCTGAGTCACAGCAACCCCACGCTCCAGGCCGTTTGTTCCTGCTGCTCCCTTTGGGATTAACTAGAGATTGAACCGGCTCACCTTGTTTCAGCTCAGGCCCTGCAACCGACCGACCGTCTGCTGGGCTCCAAGTCACCTGAGAGGCGAGGTGAGGTAAGACGGACAGTAGGGGTGGGGTAAGGCAGGCCGCTCTCTCCCATGGTGACAGGGAGCTAAGCTGAGCTGAGCCGGACTGAGCTGCTCTCCTCAGGACTCAGGGGTAGCCCTGCCCTCCCAGCTGGCTGCGATGCTCTAGTGGGACTTTCTGGAGGAACAAACATGGGAAGACATCGGGAAACCTGACAGCTTGGTCAAGAGTCTACTCGTGCCATGTGGGTGGGGGCAATTCAAGGGGTCCTTAGTGAAAAGCAGTTGGGAGCTTCTCATCTTCCCCACGCCCTCCTCTTTCCTGAGGACCACAGCAGGGGAGGGATCTGCCCAAGGACACAAGGTGACTTAGTAGAAGCAGGGCATGATGAAGATCTCTAGCCACCAACATCTGGCTAGGCCAGGGCAAAGGGACCTGGGCCATGACCTGTCTGTTTTATAAACCGAAATCGCTGCAGCCCTTGTCTTGGCATGAGGGTTTCATGGTAGAGCACGATGCCTGCTGGAGGGCCAGCGGGGGTGGGCCATCCCATCCCCAAGGGTCACTGGGGTTAAGGACCTGCCCAGACTCCCTGGGTGGAGCCCTGGGCCTTGGCAGGGGCCCAGCTGGTTCCTGGCCACTGGGGCCAGAGAACCTGGGGGCTGCTGAGTTAGTTCAGCTGAAAAACCAACAAAAGGGCCTCCCTGCTATACCCGGGGGCTCATGAGTCACCGGGGCTTTGGGGGAGTGCCAACATCATCATGACACAGCAAGAATCTTATCAGACCAATGGGGAAGTCAGCCCAGAAATGTTCTGAGGAAAGGGGAGGCGGGGGCCGCTGGGTCATCCCTCCCACAGGCCCATCATTGGGATGCGTCCTGAACGCCCATCAAGCCCATGGCCCTTGTGATCCAGGTGGGGAAACTAAGGCCCAGAGAAGTGAGGACCCCGCAGACTATCAATCCCAGTCTCTTCCCCTCACTCCCTGTGAAGCTCTCCAGCATCATCGAGGTCCCATCAGGTGGGGAAAGATGCTGTTCCAGGCGCACACTAGTCTACAAGGCCAGAGCTTTCTGGAAGGGGGCAGTAAGTACCTCGGCTCCCTTTCTGGTAGGGGTGGGAGTCCTGAGAAGGCAGGAAGTGGCCCACTTGGTAACTCTGAGGTGCCATCAGGGCCCCCAGGAAGGAAGCTGGGTGTGTGGGCAAGTGTGAGGTAAGCTGGCCAGGGAGGAGGAAGGGACAGAGGAAGGCCACGTGGGTCCAGCCTGCCCCAGGGTGTCCTGCTTGCCCAGGCTGTGGGTCTGCCAGCCACTTGCCTGCTTTCAGTTTCTAGGTCATGCTGAGCTTGTTCCCAACTCGGGGCTCCGGGCATTTGCTCTTCCTTCTGTGTTTGTGCTCTCCCGGCCCTCTTTGTGGGATCTATGCGCTTTGGGGGACTGGGGACACAGGGCCCATGTGTATCTTCTGAAACACACGTCAGCCTGAACTCTTGCTGGTCTGCTTACTTGCCGTGGTTCCCTGGCCGCAATAGCACATAGGTCCCATGAGGAGGGCAGGGACTGGCTGCTGTGGCCCAGAAGTACCCAATCGCTGTCTGCTCAATGAACGGAGAATGGGCTGCTTTCCTGGAACAGCAGATTCTAGGATCACGTGCCCTCAAGTGCCACCCTGCCTACCTCCCGCACCGAGTGAGGCATCAGGCGTGGAAGAAGCCTGGGAGCCGGAGCTGTTCCAGGTGCTGCCTGAGCACGCCACCTCCCATCTCCCCCACAGCAGGAAGAGGAAAAAACAAACCACGAGGCTCTTCAGAGAGAGGACCCCTTGTCCCCTACCCACAGTGCTGGAGCTGGCACTTCCTATTTCTGCTTTGAAAGCCTCAGGTTGTCACTCTCAGAACAGAGGAGAGCAAAGGGGAACCCTACTGATTTCAACAAAACAAAGTTGCCCAACCCGAAGCTGGCCACAGGCGGAGGCAGTGAAATGACAAAATCAGCTTGGAAAAACCTAAGGACCCTGGGCCCTCGTTTCAAAAGCTGTCATTTGCTACACGAAATGCTGAGGTTCAGGAAAAGGAAGACACTTGCTCCAACTCACACAGCAAGCTTGGATGCTCTCAATGAGGTGTCTAATAAGAGCTGAAGGCCAGGAGTCATAAGCTATCATTGTGGCCTGGGTTGCTCTGTTGCAGTGCCTTGTGACAGCATGGGGTGGGGATGGAGAAAGCAACCTCAGTTACCTTCTCCTCCAGTCTGCTTCTTGGACTAAGGGTTTAACGGTCAGAGTCCTGGCTGTTAAGGTTTGTGGCTGATGCAGGTATGGCTCTTTTTATTTTTATTTTTTTGAGATGGAGTCTCACTCACTCCGTCACCCAGGATGGAGTGCAATGGTGCCATCTCAGCTCACTGCAACCTCCACCTCCTGGGTTCAAGCGATTTTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGATGGGTGCCACTACACCTAACTAATTTTTGTTATTTTTTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCACCCAGGCTGGAATGCAGTGTTGCGATTTCTGCTCACTGCAAGCTCCGCCTCCCGAGTTCACGCCTTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGACTACAGGTGCCCACACCAGGCCTGGCTAATTTTTTATTTAGTAGAGACGGGATTTCACCATGTTAGCCAGGATGGTCTCAATCTCCTGACCTCATGATCCACCCGCTTCAGCCTCCCAAAGTGCTGGGATTACAGACGTGAGCCACTGCGCCCGGCCTTTTTGTTTTGTTTTGTTTTGAGACAGAGTCTCGCTCTGTCGCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAACCTCTGACTCCCTGATTCAAGCGATTCTACTGCCTCAGCCTACTGAGTAGCTGGGACTACAGGCACGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCTTGACCTTGTGATCTGCCCACCTTGGCCCCCCAAAGTGCTGGGATTACAGATGTGAGCCACTGCGCCCAGCCAATTTTTGTATTTTAAGTAGAGACTGGGTTTCGCCATGTTGGCCAGGCTGGTGTCAAACTCCTGACCTCAGGCGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGAATACAGGCGTGAGCCACCGTGCCCGGCCAGGTATGGCTCTTCTGAGGGGACCAGGCTGGGGCTGGGGCTGAGGCCAAGCCCAATCTACTGTGGGCTCCACCTGGTACCTCTCCTGGGTCTCAGGCTTATGGGGAGTCAGAGGACAATGGCCCCTCCTTACTCTGCCACTGGCAGAGCCCTTCTCCCTCGGCTGCCTCCTCATTCCCTTTTGGCTCTCCTTTTCTAAGTTCTGATCAGAAGTACAAAGGTGTCAAGGAGTAGGTTTGACAAAGTGTGACAGCGCGTTGTTCTATGTGAACAAAGAACCACTGAGCTCAGCCAGCACTGAGGGGCGCACGATGTGGAAGAACTAACTAGTTTTGATAGAGCTCCTGCTCAAGGTTACAAGGTAAGTTAATGGCAAAGATGGTCATACAGTAATGAGCAGAGAGATTCTTGAGTGCATCCAGGGTTAAAAGTGAAAACTGAGACCACGTGTAATTTGAGATGAAGCCCTTGTTCCACCAGCCCCTGCACAGTCTCACTGCCCCATGGGACACAGGGGAGGGTGCTCTAGTCTCGGGCGATGGCTGTCCTAGGACCACCCCTCCCTCCCCTCCCATGGAAATCCTCATGCTATACTATTCTTTTGTCTTTCCTATGAGTGCAGAATGGCGGTTCTACAAGCTGGACAATTGGGGCCAGGTGGGGTGAGGCAGAGCTCCTTGGTAGGCTTTCGAAATTGAGGCAAAGAGACAGTGTTCAAAGAAAAGCTAAGTGTTTGTATCGACGGAACTTGAAGTGTTAGTGAAGAGGCAGAGATCAGGCCTCAGATCCTGCCTTTGGAACTCATTTGTTAAGGCATGAACAGGTCTGAAAAAACTACAGAATGAATAGCATTCCCTGTTTTCCCCAAGAAGTCCATCTAGACAGTCCCTAAAGAGCCTGCAACTCCAGGATTAAGGGCTACATTCAGCGGCTAGGCACAGGGTTCAGAAACGTCCTGCAGCCGGGCGTGGTGGCTCATGCCTGTCATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGTCAACATGGTGAAACTCCGTCTCTACTAACAATACAAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATCTCAGCTACTTGGGGGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCCGAGGTTGCAGTGAGCCAAGATGGCGCCACTGCACCCCATCCTGGGCGACCAGAGCAAAACTCCGTCTCCAAAAAAAAAAAAAAAAAAAAGTCTTGCAAGTGCATATGCACACCAGGTAGAGCCGGGATGATCCTGGCGCACTAGCAGGAGCGGGAGGAGGAGCTCAACTTAGCAGAGCCTGTGGGGCCCTGCAACAATTAGTTGGAAAAGCTGAGGCATGGAGCAGGCACTTCCTGGCTTTTAAGATTGGGGCCTGGGAGATACTCACCGATGCACCCATGATGATGAATATGTGTGTATCCGACTGATGGAAGGCATCGCCCTGGAAAAGCTCTTCCCGCAGGATCCCGCACACCTGGGTCCGGCTCAGGGCCACCTGCTCTGCCATGACGCTGTCTGGTGGAAGAAAGGCTCGTTAACAAGGCAGAAGAACAGGAGAGCATTGAGAAGTTAGCCCCTTTCTTGAGAGTTCCTCTGGGGTCTCACACCAGGGTGACACCTGATTGCCCAAATCACTCCTTGTGAACGGCTGGGCATTGGGGAGTGGTTGATGGGTTAGAAACTGCTGGCTCTGGGCTCCAGCCACTCTCTCCTAGGCAGCCTTCATTCAGGGTGTACATTACAAAATTACTCAAAGCATTGGTGTATTCCGACTACAGCATCAATTCTGGACAACCGAGTAAAATCCTTGTGGGGCATGGAACTGCGTGCCCAGGACCATCTCATTCCCGACTGTAGCGGGAACTCCACAATGACCTGGAGCATGGGAGATGGTGCAGATGCTGGAGTTCAGCTCCAGCCCTCCCCTTGCCAAGCTGGGTGACCCCGGTCGGCAAGTCCCCTTCGCTCTCGGGGTCTCAGGGGCTCAAGAGAGGAGGTGCGGGGTATAAAGGGATTGGTTAAGACCCTCTCGATTCTGCTCGGTTCTCAAGCACAACAAACAGCGTGTATTTTACCGCCGCGCGGCGCAGCGCGGGACAGTACGCTCCTCCGCCTGCGCGGCGCCCGCCCGGCCGGTTACCTGCGCTTCGTCGTCGTCGCCCTCCGCGCTCGCAGCCCCGAAGTGTACGACCGTTTCCGGGGGCTGAGCCCCGCCGGCCCATTTAATCGGCGGGGGCGGGGGCGGGCGCCTGGGCTGAGCGGACCCGCCTCAGGCGAGGCGTGCGGGGCGGGGCCTCGGCCACCACCCCTCGTGCGGGCGGGGCGGGGCGAGGGCAGGTGCGCGCGCATCCCAGGCCAGCCCCTGCCTCTCGGGCACCTGCGCTGGAGGCCGGCCCGCCGGCTGCCTGCCATACCCGCTGCCGCTGCTCTGCATCCCCAATTCCGGCGGGCACGGGTGCAGCTCCGCGTAGTGCTCCCGCATCCCCATCGCCGGCCCGGCCCCGCCCCTACTGTCCGGTTTCCCCGCCTGCCCCGGCGGCCTCGCGCGCTCGCGGAGGGCTCCACTTCCGCCGGCAGCGTGGCCACGCCTCCTCTGGGCTGTCCCTCGGCTCCTGGGCGGGGCCTTCTGCCGCGCCACTCCCCCGGGAACCCTCGCCTGTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCGAGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGACTTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCACAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCCGCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGGTGTGGTCCTTTTCCCCAAAGACAGGGTTCCATCCGTGGGCGTTCCGCCGCCTCCGAAACTTCCCCCGGACGTTCAGGCTCCCCCCTCTTTTTTGGGCCCCAGCCCGTTCCTGCTCCGCGCTTCTGGAGCACTGGCCAAGGCGGGCCGATTCAGGACCCAGGTTACTTGGGCGGCGAGCTGGACTGTTTCTACTCCTCCCTCCTCCTCCACTGCGGGGTCTGACCCTACTCCTTGTGTGAGGACTCCTCTAGTTCAGAGACATATTCTGTTCACCAAACTTGACTGCGCTCTATCGAGGTCGTTAAATTCTTCGGAAATGCCTCACATATAGTTTGGCAGCTAGGTATCTGATTTCATATGCCTGTTTGCTCGTTTTGCAAGACAACATCTGCCTATCGTCATACTGTTTCTGTGATCTGAGAATGAATGGGCTCTCCTGGCACATTAGAGGAATAGCACGGAGGTCACTATAGCTGGAGCAGAGTGAGGGAGGGGAGCACAGTTGGAGAGGAAGGGATGGGGAACCCGATGGTACAGGGCCTTGTAGGCCGTTGTAGGGAGATTGGCTTTTACGCAGAAGGCAACGGGGATCCATGGTAGGGTTCTCAGTAGAGGAGGAATGTGATCGGAATTACGTCTTACACTGATCGTTCTAGCAGTGGTGGGGAGACCAGACCATAGTGGGCAAGGGTAAAAGAAGGGTGGCGTGGTAAGAGGTAATTGCAGTAATCCAGCTCAGAGACAGTGCTTTGAGCATTTTATTATTGGAAATTTCAAAAATGTACGAAAGTAGAAAAATGAGTATAGTAAATCTTTTTGGTTTGTTTGTTTTTGAGAGATGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTCTAACCTCCTCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCTTCCCAGGTAGCTGGGACTGCAGGCGTGCGCCACTACGCCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACTATATGTTGGCCAGTCTGGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCAGACTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCGCTCGGCCAGTATAGTAAATCTTACATTCACAGCTACCAACTTCAACAATTGAAGGTATCAACTTCTGACTAATTTTGTTTATATCCCTCGCAATCCTCTCCCATTATTATTTTATTTCTTTTTCTTTCTTTCTTTCTTTTTTTTTTTTTTTTGAGACAGAATCTCGCTCTGTCACCAGGCTGGAGTGCAGTGGTGTGATCTCCACCCACTGCAACTTCCGCCTCCTGGATTCAAGCGATTCTTCTGCCTCAGCCGCTGGGACTACAGTTGTGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCATCATGTTGGCCAGGATGGTCTTGATCTCTTGACCTTGTCATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCGGCCTCTTTTTTTGAGACCCAGTCTCACTCTGTCGCCCAGAATGGAGTGCAGTGGCACGATCTTGGCTCACCGCAACCTCCATCTCCCAGGTTTGAGCGATTCTCCTGCCTCAGCTTCCCAAATAGCTGGGATTACAGGCATGCACCATCACTCCCAGCTATTTTTTTTTTTTTTGTATTTTTGGTAAATACAGGGTTTTGTCATGTTGGCCAGGCTGGTCTTGAATTC = primer3-2.3.6/test/dv_conc_vs_dntp_conc_output0000664000077300007730000000232212231156245020551 0ustar ioanaioanaPRIMER_SALT_DIVALENT=1 PRIMER_DNTP_CONC=1.5 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_NUM_RETURN=1 SEQUENCE_ID=dv_conc_vs_dntp_conc SEQUENCE_TEMPLATE=atgtcaggagtgcggtaccccaccaacatatttctgctgtgcaaagactgcgaggtggatttggaagacttacgactgatttgcatttattgcacaaatgaattaacaacagcagaagtgctgtcctttgcatggaaggagctgtgtattaagtgggaccacgaactgccctacggagcg PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=2.058721 PRIMER_LEFT_0_PENALTY=0.827717 PRIMER_RIGHT_0_PENALTY=1.231004 PRIMER_LEFT_0_SEQUENCE=aggagtgcggtaccccacca PRIMER_RIGHT_0_SEQUENCE=agggcagttcgtggtcccact PRIMER_LEFT_0=5,20 PRIMER_RIGHT_0=171,21 PRIMER_LEFT_0_TM=59.172 PRIMER_RIGHT_0_TM=59.769 PRIMER_LEFT_0_GC_PERCENT=65.000 PRIMER_RIGHT_0_GC_PERCENT=61.905 PRIMER_LEFT_0_SELF_ANY_TH=14.21 PRIMER_RIGHT_0_SELF_ANY_TH=10.83 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=45.02 PRIMER_RIGHT_0_HAIRPIN_TH=41.03 PRIMER_LEFT_0_END_STABILITY=4.1700 PRIMER_RIGHT_0_END_STABILITY=4.0000 PRIMER_PAIR_0_COMPL_ANY_TH=2.83 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=167 = primer3-2.3.6/test/primer_must_use_op_input0000664000077300007730000001557512231156245020134 0ustar ioanaioanaPRIMER_SEQUENCE_ID=anyway_1 PRIMER_SHOW_OLIGO_PROBLEMS=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 PRIMER_PICK_ANYWAY=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 = PRIMER_SEQUENCE_ID=high_tm PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = PRIMER_SEQUENCE_ID=high_tm_and_self_any PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_target PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=20,122 PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_excl_region PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 EXCLUDED_REGION=20,1 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = PRIMER_SEQUENCE_ID=high_tm_and_self_any_and_incl_region PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 INCLUDED_REGION=1,160 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 = PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = PRIMER_SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc_and_target PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 143,1 PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = PRIMER_SEQUENCE_ID=internal_too_short PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 143,1 PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccat PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = PRIMER_SEQUENCE_ID=internal_ok PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 143,1 PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = PRIMER_SEQUENCE_ID=internal_too_cold PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 143,1 PRIMER_LEFT_INPUT=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_INPUT=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc PRIMER_INTERNAL_OLIGO_MIN_TM=60 PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = PRIMER_SEQUENCE_ID=internal_too_cold2 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=12 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 PRIMER_LEFT_INPUT=CCTACAGCTGTGGGAAAATG PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc PRIMER_INTERNAL_OLIGO_MIN_TM=60 PRIMER_MAX_TM=100 PRIMER_MAX_GC=100 = PRIMER_SEQUENCE_ID=mispriming PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100 PRIMER_MAX_SELF_END=12 PRIMER_NUM_RETURN=1 SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG TARGET=21,122 PRIMER_LEFT_INPUT=CCTACAGCTGTGGGAAAATG PRIMER_INTERNAL_OLIGO_INPUT=gcgtacgatccatgctagc PRIMER_INTERNAL_OLIGO_MIN_TM=60 PRIMER_MAX_TM=100 PRIMER_MAX_GC=100 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_MAX_LIBRARY_MISPRIMING=8 = primer3-2.3.6/test/primer3_io_translator.pl0000664000077300007730000001476312231156245017726 0ustar ioanaioana#!/usr/bin/perl -w # (c) Copyright 1996,1997,1998,1999,2000,2001,2004,2006,2007, 2008 # Whitehead Institute for Biomedical Research, Steve Rozen, # Andreas Untergasser and Helen Skaletsky # All rights reserved. # # This file is part of the primer3 suite and libraries. # # The primer3 suite and libraries are free software; # you can redistribute them and/or modify them under the terms # of the GNU General Public License as published by the Free # Software Foundation; either version 2 of the License, or (at # your option) any later version. # # This software is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # # You should have received a copy of the GNU General Public License # along with this software (file gpl-2.0.txt in the source # distribution); if not, write to the Free Software # Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA # # THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS # "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT # LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR # A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT # OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, # SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT # LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, # DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY # THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT # (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE # OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. use strict; use Cwd; use File::Copy; # This is what wil be changed: my %docTags = (PRIMER_NUM_NS_ACCEPTED => "PRIMER_MAX_NS_ACCEPTED", PRIMER_INTERNAL_NUM_NS_ACCEPTED => "PRIMER_INTERNAL_MAX_NS_ACCEPTED", PRIMER_MAX_DIFF_TM => "PRIMER_PAIR_MAX_DIFF_TM", PRIMER_PAIR_COMPL_ANY => "PRIMER_PAIR_MAX_COMPL_ANY", PRIMER_PAIR_COMPL_END => "PRIMER_PAIR_MAX_COMPL_END", PRIMER_SELF_ANY => "PRIMER_MAX_SELF_ANY", PRIMER_INTERNAL_SELF_ANY => "PRIMER_INTERNAL_MAX_SELF_ANY", PRIMER_SELF_END => "PRIMER_MAX_SELF_END", PRIMER_INTERNAL_SELF_END => "PRIMER_INTERNAL_MAX_SELF_END"); # Directory Name my $directory = cwd; my @directoryFiles; if ( opendir(DIR, $directory)) { push @directoryFiles, readdir(DIR); closedir(DIR); } else { print "Couldn't open $directory for reading!\n"; } print ("Start Translation...\n"); my $input; my $output; foreach my $si_file (@directoryFiles) { if (($si_file eq ".") or ($si_file eq "..") or ($si_file eq ".svn") or ($si_file =~ /_formatted_output$/) or ($si_file =~ /^dpal_/)) { next; } # print $si_file."\n"; if ($si_file =~ /.in$/) { # print $si_file."\n"; $input = $si_file; $output = $si_file; translate_file($input,$output); } if ($si_file =~ /.out$/) { # print $si_file."\n"; $input = $si_file; $output = $si_file; translate_file($input,$output); } if ($si_file =~ /_input$/) { # print $si_file."\n"; $input = $si_file; $output = $si_file; translate_file($input,$output); } if ($si_file =~ /_output$/) { # print $si_file."\n"; $input = $si_file; $output = $si_file; translate_file($input,$output); } } #translate_file("primer_task_input","primer_task_input_tr"); print ("\nTranslation finished\n"); ########################################## # Reads a File into a string and returns # # 0 for success # # or error message # ########################################## sub translate_file { my $file = shift; # File to read my $target = shift; # File to save in my $fin_target = $target; my $error = 0; my $text_input = ""; my $text_output = ""; my @string_array; my @lineA; # Read the file in a string $error = read2String(\$file, \$text_input); if ($error ne "0"){ print $error; return $error; } my $backup = $file."_bakup"; # $error = string2file($backup, $text_input); if ($error ne "0"){ print $error; } # Split the string into single lines @string_array = split '\n', $text_input; # Handle each line separate foreach my $line (@string_array) { # First handle the = only lines if ($line eq "="){ $text_output .= "=\n"; } # Now handle lines ending with = elsif ($line =~ /=$/) { $line =~ s/=$//; $text_output .= $line."=\n"; } # Catch lines without a = (should not happen) elsif (!($line =~ /=/)) { print "\nError in $file at Line: ". $line."\n\n"; $fin_target = $target."_error"; } # Translate the old tags into new tags else { @lineA = split '=', $line; if (defined $docTags{$lineA[0]}){ $text_output .= $docTags{$lineA[0]}."=".$lineA[1]."\n"; } else { $text_output .= $lineA[0]."=".$lineA[1]."\n"; } } } # $text_output # Save the file $error = string2file($fin_target, $text_output); if ($error ne "0"){ print $error; } return $error; } ########################################## # Reads a File into a string and returns # # 0 for success # # or error message # ########################################## sub read2String { my $file = shift; # File Name my $target = shift; # String to save in my $error = "0"; if (open (FILE, ("<".${$file}))) { while () { ${$target} .= $_; } close(FILE); # solve the newline problem with other platforms if ( ${$target} =~ /\r\n/ ) { ${$target} =~ s/\r\n/\n/g; } if ( ${$target} =~ /\r/ ) { ${$target} =~ s/\r/\n/g; } } else { $error = "Couldn't open ${$file} for reading!\n"; } return $error; } ########################################## # Writes a string int a File and returns # # 0 for success # # or error message # ########################################## sub string2file { my $file = shift; # File Name my $string = shift; # String to save in my $error = 0; if (open FILE, ">", $file) { print FILE $string; close(FILE); } else { $error = "Couldn't open $file for writing!\n"; } return $error; } primer3-2.3.6/test/dv_conc_vs_dntp_conc_input0000664000077300007730000000065112231156245020353 0ustar ioanaioanaPRIMER_SALT_DIVALENT=1 PRIMER_DNTP_CONC=1.5 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_NUM_RETURN=1 SEQUENCE_ID=dv_conc_vs_dntp_conc SEQUENCE_TEMPLATE=atgtcaggagtgcggtaccccaccaacatatttctgctgtgcaaagactgcgaggtggatttggaagacttacgactgatttgcatttattgcacaaatgaattaacaacagcagaagtgctgtcctttgcatggaaggagctgtgtattaagtgggaccacgaactgccctacggagcg = primer3-2.3.6/test/primer_new_tasks_th_input0000664000077300007730000007677212231156245020271 0ustar ioanaioanaPRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 SEQUENCE_ID=Check left only SEQUENCE_PRIMER=gtcctcgaacagctcagtcc PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 PRIMER_DNTP_CONC=0.0 = SEQUENCE_ID=Check internal only SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Check right only SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick the same primers as before SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=1 = SEQUENCE_ID=Check primers with sequence SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Check primers without sequence PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Check primers without sequence and internal PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick three times the same primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=5 = SEQUENCE_ID=Make a list of a small range SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatga PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=20 = SEQUENCE_ID=Make a list of a small range SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatga PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=2 = SEQUENCE_ID=Check left only too small SEQUENCE_PRIMER=gtcctcgaaca PRIMER_TASK=check_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Check left only too big SEQUENCE_PRIMER=tgacctccacttctctggacacagctccctcaccaa PRIMER_TASK=check_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick sequencing primers no target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=50 = SEQUENCE_ID=Pick sequencing primers small target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=450,37 PRIMER_NUM_RETURN=50 = SEQUENCE_ID=Pick sequencing primers left only SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=150,800 PRIMER_NUM_RETURN=50 = SEQUENCE_ID=Pick sequencing primers right only SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 SEQUENCE_TARGET=150,800 PRIMER_NUM_RETURN=50 = SEQUENCE_ID=Pick sequencing primers three target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=350,37 450,100 850,500 1200,100 PRIMER_NUM_RETURN=50 = SEQUENCE_ID=Pick fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 SEQUENCE_FORCE_LEFT_END=34 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_START=697 SEQUENCE_FORCE_RIGHT_END=678 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick start fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick start fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_START=697 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick end fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_END=34 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick end fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_END=678 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick cloning primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_cloning_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_INCLUDED_REGION=15,203 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick discriminative primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_discriminative_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=35,163 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 = SEQUENCE_ID=Pick start fixed left primer unique SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 PRIMER_NUM_RETURN=3 PRIMER_MIN_THREE_PRIME_DISTANCE=0 PRIMER_PICK_ANYWAY=1 = primer3-2.3.6/test/cmd_test5_output0000664000077300007730000000424512231156245016274 0ustar ioanaioana Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 2009,2010,2011,2012 Whitehead Institute for Biomedical Research, Steve Rozen (http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky All rights reserved. This file is part of the primer3 suite and libraries. The primer3 suite and libraries are free software; you can redistribute them and/or modify them under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This software is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this software (file gpl-2.0.txt in the source distribution); if not, write to the Free Software Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. USAGE: ../src/primer3_core [-format_output] [-default_version=1|-default_version=2] [-io_version=4] [-p3_settings_file=] [-echo_settings_file] [-strict_tags] [-output=] [-error=] [input_file] This is primer3 (libprimer3 release 2.3.5) Input can also be provided on standard input. For example: $ primer3_core < my_input_file primer3-2.3.6/test/primer_repeat_boundary2_lib0000664000077300007730000000046712231156245020440 0ustar ioanaioana>test_1 * 2.0 TATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATAT primer3-2.3.6/test/primer_mispriming_long_lib_input0000664000077300007730000000134612231156245021604 0ustar ioanaioanaSEQUENCE_ID=seq0 P3_COMMENT=should fail PRIMER_MAX_LIBRARY_MISPRIMING=18 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=20 PRIMER_PAIR_MAX_COMPL_ANY=20 PRIMER_PAIR_MAX_COMPL_END=20 PRIMER_MAX_TM=100 PRIMER_MIN_TM=10 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_MIN_GC=10 PRIMER_MAX_GC=90 SEQUENCE_TEMPLATE=NACGGGCATGGCGATCGCTGGNNNNNNNNNNNNANNNNNNNNCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNANNNNNNNNCNNNNNNNNTNCNGCTGGCAGTCTAGCGGGCATNANANANANAAN PRIMER_MISPRIMING_LIBRARY=primer_repeat_long_lib PRIMER_EXPLAIN_FLAG=1 = SEQUENCE_ID=seq1 P3_COMMENT=Should find one pair SEQUENCE_TEMPLATE=NACGGGCATGGCGATCGCTAANNNNNNNNNNNNANNNNNNNNCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNANNNNNNNNCNNNNNNNNTNCNGCTGGCAGTCTAGCGGGCATNANANANANAAN = primer3-2.3.6/test/primer_mispriming_boundary1_formatted_output0000664000077300007730000001132412231156245024166 0ustar ioanaioanaPRIMER PICKING RESULTS FOR pair_1 Using mispriming library primer_repeat_boundary1_lib Using internal oligo mishyb library primer_repeat_boundary1_lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC RIGHT PRIMER 181 20 91.74 100.00 14.00 14.00 0.00 GCGCGCGCGCGCGCCGCGCG INTERNAL OLIGO 111 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC SEQUENCE SIZE: 182 INCLUDED REGION SIZE: 182 PRODUCT SIZE: 182, PAIR ANY COMPL: 17.00, PAIR 3' COMPL: 17.00 0 GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATAT >>>>>>>>>>>>>>>>>>>> 60 ATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCG ^^^^^^^^^ 120 CGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGC ^^^^^^^^^^^ <<<<<<<<<<<<<<<<<< 180 GC << KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 83 0 0 0 0 0 0 0 0 0 0 82 0 0 1 Right 83 0 0 0 0 0 0 0 0 0 0 71 0 0 12 Intl 163 0 0 0 0 0 0 0 0 0 0 121 0 0 42 Pair Stats: considered 381, unacceptable product size 369, no internal oligo 11, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR pair_0 Using mispriming library primer_repeat_boundary1_lib Using internal oligo mishyb library primer_repeat_boundary1_lib Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 163 INCLUDED REGION SIZE: 163 0 TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA 60 TATATATATATTATATATATATATATATATATATATATATATATATATATATATATATAT 120 ATATATATATATATATATATATATATATATATATATATATATA Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 64 0 0 0 0 0 0 0 0 0 0 64 0 0 0 Right 64 0 0 0 0 0 0 0 0 0 0 0 0 0 64 Intl 144 0 0 0 0 0 0 0 0 0 0 0 0 0 144 Pair Stats: considered 32, unacceptable product size 32, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR pair_1 Using mispriming library primer_repeat_boundary1_lib Using internal oligo mishyb library primer_repeat_boundary1_lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC RIGHT PRIMER 181 20 91.74 100.00 14.00 14.00 0.00 GCGCGCGCGCGCGCCGCGCG INTERNAL OLIGO 111 20 91.85 100.00 20.00 20.00 0.00 GCGCGCGCGCGCGCGCGCGC SEQUENCE SIZE: 182 INCLUDED REGION SIZE: 182 PRODUCT SIZE: 182, PAIR ANY COMPL: 17.00, PAIR 3' COMPL: 17.00 0 GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATAT >>>>>>>>>>>>>>>>>>>> 60 ATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCG ^^^^^^^^^ 120 CGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGC ^^^^^^^^^^^ <<<<<<<<<<<<<<<<<< 180 GC << KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 83 0 0 0 0 0 0 0 0 0 0 0 0 0 83 Right 83 0 0 0 0 0 0 0 0 0 0 0 0 0 83 Intl 163 0 0 0 0 0 0 0 0 0 0 121 0 0 42 Pair Stats: considered 6889, unacceptable product size 3403, no internal oligo 11, high mispriming library similarity 3474, ok 1 primer3 release 2.0.0 primer3-2.3.6/test/primer_mispriming_boundary1_output0000664000077300007730000001120612231156245022120 0ustar ioanaioanaSEQUENCE_ID=pair_1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY=primer_repeat_boundary1_lib PRIMER_INTERNAL_MISHYB_LIBRARY=primer_repeat_boundary1_lib SEQUENCE_TEMPLATE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_LIBRARY_MISPRIMING=0 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=0 PRIMER_MAX_SELF_ANY=25 PRIMER_MAX_SELF_END=25 PRIMER_PAIR_MAX_COMPL_ANY=25 PRIMER_PAIR_MAX_COMPL_END=25 PRIMER_INTERNAL_MAX_SELF_ANY=25 PRIMER_INTERNAL_MAX_SELF_END=25 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_SIZE=20 PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_INTERNAL_MAX_GC=100 PRIMER_MAX_SIZE=20 PRIMER_MIN_SIZE=20 PRIMER_OPT_SIZE=20 PRIMER_MAX_TM=100 PRIMER_MIN_TM=0 PRIMER_OPT_TM=60 PRIMER_LEFT_EXPLAIN=considered 83, high repeat similarity 82, ok 1 PRIMER_RIGHT_EXPLAIN=considered 83, high repeat similarity 71, ok 12 PRIMER_INTERNAL_EXPLAIN=considered 163, high repeat similarity 121, ok 42 PRIMER_PAIR_EXPLAIN=considered 381, unacceptable product size 369, no internal oligo 11, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=63.584014 PRIMER_LEFT_0_PENALTY=31.846382 PRIMER_RIGHT_0_PENALTY=31.737633 PRIMER_INTERNAL_0_PENALTY=31.846382 PRIMER_LEFT_0_SEQUENCE=GCGCGCGCGCGCGCGCGCGC PRIMER_RIGHT_0_SEQUENCE=GCGCGCGCGCGCGCCGCGCG PRIMER_INTERNAL_0_SEQUENCE=GCGCGCGCGCGCGCGCGCGC PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=181,20 PRIMER_INTERNAL_0=111,20 PRIMER_LEFT_0_TM=91.846 PRIMER_RIGHT_0_TM=91.738 PRIMER_INTERNAL_0_TM=91.846 PRIMER_LEFT_0_GC_PERCENT=100.000 PRIMER_RIGHT_0_GC_PERCENT=100.000 PRIMER_INTERNAL_0_GC_PERCENT=100.000 PRIMER_LEFT_0_SELF_ANY=20.00 PRIMER_RIGHT_0_SELF_ANY=14.00 PRIMER_INTERNAL_0_SELF_ANY=20.00 PRIMER_LEFT_0_SELF_END=20.00 PRIMER_RIGHT_0_SELF_END=14.00 PRIMER_INTERNAL_0_SELF_END=20.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=0.00, test_1 PRIMER_RIGHT_0_LIBRARY_MISPRIMING=0.00, test_1 PRIMER_PAIR_0_LIBRARY_MISPRIMING=0.00, test_1 PRIMER_INTERNAL_0_LIBRARY_MISHYB=0.00, test_1 PRIMER_LEFT_0_END_STABILITY=13.4000 PRIMER_RIGHT_0_END_STABILITY=13.4000 PRIMER_PAIR_0_COMPL_ANY=17.00 PRIMER_PAIR_0_COMPL_END=17.00 PRIMER_PAIR_0_PRODUCT_SIZE=182 = SEQUENCE_ID=pair_0 P3_COMMENT="Should fail because of high repeat similarity" SEQUENCE_TEMPLATE=TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA PRIMER_LEFT_EXPLAIN=considered 64, high repeat similarity 64, ok 0 PRIMER_RIGHT_EXPLAIN=considered 64, ok 64 PRIMER_INTERNAL_EXPLAIN=considered 144, ok 144 PRIMER_PAIR_EXPLAIN=considered 32, unacceptable product size 32, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=pair_1 SEQUENCE_TEMPLATE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=0 PRIMER_MAX_LIBRARY_MISPRIMING=20 PRIMER_LEFT_EXPLAIN=considered 83, ok 83 PRIMER_RIGHT_EXPLAIN=considered 83, ok 83 PRIMER_INTERNAL_EXPLAIN=considered 163, high repeat similarity 121, ok 42 PRIMER_PAIR_EXPLAIN=considered 6889, unacceptable product size 3403, no internal oligo 11, high mispriming library similarity 3474, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=63.584014 PRIMER_LEFT_0_PENALTY=31.846382 PRIMER_RIGHT_0_PENALTY=31.737633 PRIMER_INTERNAL_0_PENALTY=31.846382 PRIMER_LEFT_0_SEQUENCE=GCGCGCGCGCGCGCGCGCGC PRIMER_RIGHT_0_SEQUENCE=GCGCGCGCGCGCGCCGCGCG PRIMER_INTERNAL_0_SEQUENCE=GCGCGCGCGCGCGCGCGCGC PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=181,20 PRIMER_INTERNAL_0=111,20 PRIMER_LEFT_0_TM=91.846 PRIMER_RIGHT_0_TM=91.738 PRIMER_INTERNAL_0_TM=91.846 PRIMER_LEFT_0_GC_PERCENT=100.000 PRIMER_RIGHT_0_GC_PERCENT=100.000 PRIMER_INTERNAL_0_GC_PERCENT=100.000 PRIMER_LEFT_0_SELF_ANY=20.00 PRIMER_RIGHT_0_SELF_ANY=14.00 PRIMER_INTERNAL_0_SELF_ANY=20.00 PRIMER_LEFT_0_SELF_END=20.00 PRIMER_RIGHT_0_SELF_END=14.00 PRIMER_INTERNAL_0_SELF_END=20.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=0.00, test_1 PRIMER_RIGHT_0_LIBRARY_MISPRIMING=0.00, test_1 PRIMER_PAIR_0_LIBRARY_MISPRIMING=0.00, test_1 PRIMER_INTERNAL_0_LIBRARY_MISHYB=0.00, test_1 PRIMER_LEFT_0_END_STABILITY=13.4000 PRIMER_RIGHT_0_END_STABILITY=13.4000 PRIMER_PAIR_0_COMPL_ANY=17.00 PRIMER_PAIR_0_COMPL_END=17.00 PRIMER_PAIR_0_PRODUCT_SIZE=182 = primer3-2.3.6/test/primer_must_match_output0000664000077300007730000031403212231156245020125 0ustar ioanaioanaSEQUENCE_ID=Pick Primers as a reference SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=25 PRIMER_TASK=generic PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 PRIMER_LEFT_EXPLAIN=considered 8496, GC content failed 704, low tm 5558, high tm 529, high end compl 10, long poly-x seq 92, ok 1603 PRIMER_RIGHT_EXPLAIN=considered 8494, GC content failed 723, low tm 5642, high tm 406, high end compl 10, long poly-x seq 92, ok 1621 PRIMER_INTERNAL_EXPLAIN=considered 9271, GC content failed 725, low tm 6079, high tm 542, long poly-x seq 92, ok 1833 PRIMER_PAIR_EXPLAIN=considered 2657, unacceptable product size 2620, high end compl 1, primer in pair overlaps a primer in a better pair 2423, ok 36 PRIMER_LEFT_NUM_RETURNED=25 PRIMER_RIGHT_NUM_RETURNED=25 PRIMER_INTERNAL_NUM_RETURNED=25 PRIMER_PAIR_NUM_RETURNED=25 PRIMER_PAIR_0_PENALTY=1.171882 PRIMER_LEFT_0_PENALTY=0.117097 PRIMER_RIGHT_0_PENALTY=1.054785 PRIMER_INTERNAL_0_PENALTY=1.344173 PRIMER_LEFT_0_SEQUENCE=attcgattctccccggtatc PRIMER_RIGHT_0_SEQUENCE=tgcatgattggatacgtttga PRIMER_INTERNAL_0_SEQUENCE=tccctccacattctctttgaa PRIMER_LEFT_0=61,20 PRIMER_RIGHT_0=352,21 PRIMER_INTERNAL_0=307,21 PRIMER_LEFT_0_TM=60.117 PRIMER_RIGHT_0_TM=59.945 PRIMER_INTERNAL_0_TM=59.656 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_INTERNAL_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.3000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=292 PRIMER_PAIR_1_PENALTY=1.389004 PRIMER_LEFT_1_PENALTY=1.344173 PRIMER_RIGHT_1_PENALTY=0.044832 PRIMER_INTERNAL_1_PENALTY=1.054785 PRIMER_LEFT_1_SEQUENCE=tccctccacattctctttgaa PRIMER_RIGHT_1_SEQUENCE=ggctgatgaaggaaagcaag PRIMER_INTERNAL_1_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_1=307,21 PRIMER_RIGHT_1=528,20 PRIMER_INTERNAL_1=332,21 PRIMER_LEFT_1_TM=59.656 PRIMER_RIGHT_1_TM=59.955 PRIMER_INTERNAL_1_TM=59.945 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=222 PRIMER_PAIR_2_PENALTY=1.461269 PRIMER_LEFT_2_PENALTY=0.117097 PRIMER_RIGHT_2_PENALTY=1.344173 PRIMER_INTERNAL_2_PENALTY=5.848837 PRIMER_LEFT_2_SEQUENCE=gattcgattctccccggtat PRIMER_RIGHT_2_SEQUENCE=tcaaagagaatgtggagggaa PRIMER_INTERNAL_2_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_2=60,20 PRIMER_RIGHT_2=326,21 PRIMER_INTERNAL_2=273,24 PRIMER_LEFT_2_TM=60.117 PRIMER_RIGHT_2_TM=59.656 PRIMER_INTERNAL_2_TM=58.151 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.8000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=267 PRIMER_PAIR_3_PENALTY=2.071477 PRIMER_LEFT_3_PENALTY=1.344173 PRIMER_RIGHT_3_PENALTY=0.727304 PRIMER_INTERNAL_3_PENALTY=1.054785 PRIMER_LEFT_3_SEQUENCE=ttccctccacattctctttga PRIMER_RIGHT_3_SEQUENCE=aatggctgatgaaggaaagc PRIMER_INTERNAL_3_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_3=306,21 PRIMER_RIGHT_3=531,20 PRIMER_INTERNAL_3=332,21 PRIMER_LEFT_3_TM=59.656 PRIMER_RIGHT_3_TM=59.273 PRIMER_INTERNAL_3_TM=59.945 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=226 PRIMER_PAIR_4_PENALTY=2.122810 PRIMER_LEFT_4_PENALTY=1.058858 PRIMER_RIGHT_4_PENALTY=1.063952 PRIMER_INTERNAL_4_PENALTY=4.596606 PRIMER_LEFT_4_SEQUENCE=cccatgtgaatgtattggagg PRIMER_RIGHT_4_SEQUENCE=tcaacagttgtcaaacttgcg PRIMER_INTERNAL_4_SEQUENCE=ggagacgattatgatttaattgca PRIMER_LEFT_4=832,21 PRIMER_RIGHT_4=948,21 PRIMER_INTERNAL_4=893,24 PRIMER_LEFT_4_TM=60.059 PRIMER_RIGHT_4_TM=59.936 PRIMER_INTERNAL_4_TM=59.403 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=10.5000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=117 PRIMER_PAIR_5_PENALTY=2.131001 PRIMER_LEFT_5_PENALTY=0.786828 PRIMER_RIGHT_5_PENALTY=1.344173 PRIMER_INTERNAL_5_PENALTY=5.848837 PRIMER_LEFT_5_SEQUENCE=agattcgattctccccggta PRIMER_RIGHT_5_SEQUENCE=ttcaaagagaatgtggaggga PRIMER_INTERNAL_5_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_5=59,20 PRIMER_RIGHT_5=327,21 PRIMER_INTERNAL_5=273,24 PRIMER_LEFT_5_TM=60.787 PRIMER_RIGHT_5_TM=59.656 PRIMER_INTERNAL_5_TM=58.151 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=42.857 PRIMER_INTERNAL_5_GC_PERCENT=37.500 PRIMER_LEFT_5_SELF_ANY=8.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_INTERNAL_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_INTERNAL_5_SELF_END=1.00 PRIMER_LEFT_5_END_STABILITY=8.9000 PRIMER_RIGHT_5_END_STABILITY=9.4000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=269 PRIMER_PAIR_6_PENALTY=2.227098 PRIMER_LEFT_6_PENALTY=1.482841 PRIMER_RIGHT_6_PENALTY=0.744257 PRIMER_INTERNAL_6_PENALTY=3.156485 PRIMER_LEFT_6_SEQUENCE=tccaatcatgcatgtctgttc PRIMER_RIGHT_6_SEQUENCE=atggctgatgaaggaaagca PRIMER_INTERNAL_6_SEQUENCE=tgatcataacagcaaaagcatg PRIMER_LEFT_6=341,21 PRIMER_RIGHT_6=530,20 PRIMER_INTERNAL_6=362,22 PRIMER_LEFT_6_TM=59.517 PRIMER_RIGHT_6_TM=60.744 PRIMER_INTERNAL_6_TM=58.844 PRIMER_LEFT_6_GC_PERCENT=42.857 PRIMER_RIGHT_6_GC_PERCENT=45.000 PRIMER_INTERNAL_6_GC_PERCENT=36.364 PRIMER_LEFT_6_SELF_ANY=8.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_INTERNAL_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_INTERNAL_6_SELF_END=4.00 PRIMER_LEFT_6_END_STABILITY=6.7000 PRIMER_RIGHT_6_END_STABILITY=8.5000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=190 PRIMER_PAIR_7_PENALTY=2.282031 PRIMER_LEFT_7_PENALTY=0.044832 PRIMER_RIGHT_7_PENALTY=2.237199 PRIMER_INTERNAL_7_PENALTY=3.879062 PRIMER_LEFT_7_SEQUENCE=cttgctttccttcatcagcc PRIMER_RIGHT_7_SEQUENCE=tcacacccttttacacatttgg PRIMER_INTERNAL_7_SEQUENCE=ttgttcattctgtttctggattg PRIMER_LEFT_7=509,20 PRIMER_RIGHT_7=657,22 PRIMER_INTERNAL_7=593,23 PRIMER_LEFT_7_TM=59.955 PRIMER_RIGHT_7_TM=59.763 PRIMER_INTERNAL_7_TM=59.121 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=40.909 PRIMER_INTERNAL_7_GC_PERCENT=34.783 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_INTERNAL_7_SELF_ANY=3.00 PRIMER_LEFT_7_SELF_END=1.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_INTERNAL_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=8.8000 PRIMER_PAIR_7_COMPL_ANY=2.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PAIR_7_PRODUCT_SIZE=149 PRIMER_PAIR_8_PENALTY=2.676544 PRIMER_LEFT_8_PENALTY=0.833045 PRIMER_RIGHT_8_PENALTY=1.843499 PRIMER_INTERNAL_8_PENALTY=5.848837 PRIMER_LEFT_8_SEQUENCE=tctcagattcgattctcccc PRIMER_RIGHT_8_SEQUENCE=tttcaaagagaatgtggaggg PRIMER_INTERNAL_8_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_8=55,20 PRIMER_RIGHT_8=328,21 PRIMER_INTERNAL_8=273,24 PRIMER_LEFT_8_TM=59.167 PRIMER_RIGHT_8_TM=59.157 PRIMER_INTERNAL_8_TM=58.151 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=42.857 PRIMER_INTERNAL_8_GC_PERCENT=37.500 PRIMER_LEFT_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_INTERNAL_8_SELF_ANY=4.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_INTERNAL_8_SELF_END=1.00 PRIMER_LEFT_8_END_STABILITY=10.9000 PRIMER_RIGHT_8_END_STABILITY=9.4000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PAIR_8_PRODUCT_SIZE=274 PRIMER_PAIR_9_PENALTY=3.041124 PRIMER_LEFT_9_PENALTY=1.029407 PRIMER_RIGHT_9_PENALTY=2.011716 PRIMER_INTERNAL_9_PENALTY=1.344173 PRIMER_LEFT_9_SEQUENCE=ggtctcagattcgattctccc PRIMER_RIGHT_9_SEQUENCE=tgcatgattggatacgtttg PRIMER_INTERNAL_9_SEQUENCE=tccctccacattctctttgaa PRIMER_LEFT_9=53,21 PRIMER_RIGHT_9=352,20 PRIMER_INTERNAL_9=307,21 PRIMER_LEFT_9_TM=60.029 PRIMER_RIGHT_9_TM=57.988 PRIMER_INTERNAL_9_TM=59.656 PRIMER_LEFT_9_GC_PERCENT=52.381 PRIMER_RIGHT_9_GC_PERCENT=40.000 PRIMER_INTERNAL_9_GC_PERCENT=42.857 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_INTERNAL_9_SELF_ANY=3.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_INTERNAL_9_SELF_END=3.00 PRIMER_LEFT_9_END_STABILITY=9.4000 PRIMER_RIGHT_9_END_STABILITY=7.0000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=300 PRIMER_PAIR_10_PENALTY=3.057112 PRIMER_LEFT_10_PENALTY=1.033556 PRIMER_RIGHT_10_PENALTY=2.023556 PRIMER_INTERNAL_10_PENALTY=5.848837 PRIMER_LEFT_10_SEQUENCE=cgattctccccggtatcaat PRIMER_RIGHT_10_SEQUENCE=aagagaatgtggagggaacaaa PRIMER_INTERNAL_10_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_10=64,20 PRIMER_RIGHT_10=323,22 PRIMER_INTERNAL_10=273,24 PRIMER_LEFT_10_TM=61.034 PRIMER_RIGHT_10_TM=59.976 PRIMER_INTERNAL_10_TM=58.151 PRIMER_LEFT_10_GC_PERCENT=50.000 PRIMER_RIGHT_10_GC_PERCENT=40.909 PRIMER_INTERNAL_10_GC_PERCENT=37.500 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=3.00 PRIMER_INTERNAL_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_INTERNAL_10_SELF_END=1.00 PRIMER_LEFT_10_END_STABILITY=6.9000 PRIMER_RIGHT_10_END_STABILITY=7.0000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=260 PRIMER_PAIR_11_PENALTY=3.140307 PRIMER_LEFT_11_PENALTY=2.023556 PRIMER_RIGHT_11_PENALTY=1.116751 PRIMER_INTERNAL_11_PENALTY=1.054785 PRIMER_LEFT_11_SEQUENCE=ttgttccctccacattctcttt PRIMER_RIGHT_11_SEQUENCE=tgaaggaaagcaagaagcaaa PRIMER_INTERNAL_11_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_11=303,22 PRIMER_RIGHT_11=522,21 PRIMER_INTERNAL_11=332,21 PRIMER_LEFT_11_TM=59.976 PRIMER_RIGHT_11_TM=60.117 PRIMER_INTERNAL_11_TM=59.945 PRIMER_LEFT_11_GC_PERCENT=40.909 PRIMER_RIGHT_11_GC_PERCENT=38.095 PRIMER_INTERNAL_11_GC_PERCENT=38.095 PRIMER_LEFT_11_SELF_ANY=3.00 PRIMER_RIGHT_11_SELF_ANY=2.00 PRIMER_INTERNAL_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_INTERNAL_11_SELF_END=4.00 PRIMER_LEFT_11_END_STABILITY=7.0000 PRIMER_RIGHT_11_END_STABILITY=8.8000 PRIMER_PAIR_11_COMPL_ANY=6.00 PRIMER_PAIR_11_COMPL_END=3.00 PRIMER_PAIR_11_PRODUCT_SIZE=220 PRIMER_PAIR_12_PENALTY=3.252729 PRIMER_LEFT_12_PENALTY=2.023556 PRIMER_RIGHT_12_PENALTY=1.229173 PRIMER_INTERNAL_12_PENALTY=1.054785 PRIMER_LEFT_12_SEQUENCE=tttgttccctccacattctctt PRIMER_RIGHT_12_SEQUENCE=tgaaggaaagcaagaagcaa PRIMER_INTERNAL_12_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_12=302,22 PRIMER_RIGHT_12=522,20 PRIMER_INTERNAL_12=332,21 PRIMER_LEFT_12_TM=59.976 PRIMER_RIGHT_12_TM=58.771 PRIMER_INTERNAL_12_TM=59.945 PRIMER_LEFT_12_GC_PERCENT=40.909 PRIMER_RIGHT_12_GC_PERCENT=40.000 PRIMER_INTERNAL_12_GC_PERCENT=38.095 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=2.00 PRIMER_INTERNAL_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_INTERNAL_12_SELF_END=4.00 PRIMER_LEFT_12_END_STABILITY=6.7000 PRIMER_RIGHT_12_END_STABILITY=8.5000 PRIMER_PAIR_12_COMPL_ANY=6.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PAIR_12_PRODUCT_SIZE=221 PRIMER_PAIR_13_PENALTY=3.299314 PRIMER_LEFT_13_PENALTY=1.188029 PRIMER_RIGHT_13_PENALTY=2.111285 PRIMER_INTERNAL_13_PENALTY=3.306406 PRIMER_LEFT_13_SEQUENCE=atcaacgttccgttcccat PRIMER_RIGHT_13_SEQUENCE=caacagttgtcaaacttgcgt PRIMER_INTERNAL_13_SEQUENCE=ttggaggtgaatttctaggaca PRIMER_LEFT_13=818,19 PRIMER_RIGHT_13=947,21 PRIMER_INTERNAL_13=846,22 PRIMER_LEFT_13_TM=60.188 PRIMER_RIGHT_13_TM=58.889 PRIMER_INTERNAL_13_TM=58.694 PRIMER_LEFT_13_GC_PERCENT=47.368 PRIMER_RIGHT_13_GC_PERCENT=42.857 PRIMER_INTERNAL_13_GC_PERCENT=40.909 PRIMER_LEFT_13_SELF_ANY=6.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_INTERNAL_13_SELF_ANY=5.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_INTERNAL_13_SELF_END=2.00 PRIMER_LEFT_13_END_STABILITY=9.6000 PRIMER_RIGHT_13_END_STABILITY=9.9000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=0.00 PRIMER_PAIR_13_PRODUCT_SIZE=130 PRIMER_PAIR_14_PENALTY=3.404939 PRIMER_LEFT_14_PENALTY=2.237199 PRIMER_RIGHT_14_PENALTY=1.167740 PRIMER_INTERNAL_14_PENALTY=4.438896 PRIMER_LEFT_14_SEQUENCE=ccaaatgtgtaaaagggtgtga PRIMER_RIGHT_14_SEQUENCE=cattcacatgggaacggaac PRIMER_INTERNAL_14_SEQUENCE=gcaatcaaagattgttcttaccaa PRIMER_LEFT_14=636,22 PRIMER_RIGHT_14=843,20 PRIMER_INTERNAL_14=721,24 PRIMER_LEFT_14_TM=59.763 PRIMER_RIGHT_14_TM=61.168 PRIMER_INTERNAL_14_TM=59.561 PRIMER_LEFT_14_GC_PERCENT=40.909 PRIMER_RIGHT_14_GC_PERCENT=50.000 PRIMER_INTERNAL_14_GC_PERCENT=33.333 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=8.00 PRIMER_INTERNAL_14_SELF_ANY=7.00 PRIMER_LEFT_14_SELF_END=1.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_INTERNAL_14_SELF_END=3.00 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=7.9000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=0.00 PRIMER_PAIR_14_PRODUCT_SIZE=208 PRIMER_PAIR_15_PENALTY=3.404939 PRIMER_LEFT_15_PENALTY=2.237199 PRIMER_RIGHT_15_PENALTY=1.167740 PRIMER_INTERNAL_15_PENALTY=4.438896 PRIMER_LEFT_15_SEQUENCE=tccaaatgtgtaaaagggtgtg PRIMER_RIGHT_15_SEQUENCE=acattcacatgggaacggaa PRIMER_INTERNAL_15_SEQUENCE=gcaatcaaagattgttcttaccaa PRIMER_LEFT_15=635,22 PRIMER_RIGHT_15=844,20 PRIMER_INTERNAL_15=721,24 PRIMER_LEFT_15_TM=59.763 PRIMER_RIGHT_15_TM=61.168 PRIMER_INTERNAL_15_TM=59.561 PRIMER_LEFT_15_GC_PERCENT=40.909 PRIMER_RIGHT_15_GC_PERCENT=45.000 PRIMER_INTERNAL_15_GC_PERCENT=33.333 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=8.00 PRIMER_INTERNAL_15_SELF_ANY=7.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_INTERNAL_15_SELF_END=3.00 PRIMER_LEFT_15_END_STABILITY=6.4000 PRIMER_RIGHT_15_END_STABILITY=10.2000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=210 PRIMER_PAIR_16_PENALTY=3.695091 PRIMER_LEFT_16_PENALTY=2.278124 PRIMER_RIGHT_16_PENALTY=1.416967 PRIMER_INTERNAL_16_PENALTY=3.541086 PRIMER_LEFT_16_SEQUENCE=caatcatgcatgtctgttctga PRIMER_RIGHT_16_SEQUENCE=gatgaaggaaagcaagaagca PRIMER_INTERNAL_16_SEQUENCE=tcataacagcaaaagcatgtg PRIMER_LEFT_16=343,22 PRIMER_RIGHT_16=524,21 PRIMER_INTERNAL_16=365,21 PRIMER_LEFT_16_TM=59.722 PRIMER_RIGHT_16_TM=59.583 PRIMER_INTERNAL_16_TM=57.459 PRIMER_LEFT_16_GC_PERCENT=40.909 PRIMER_RIGHT_16_GC_PERCENT=42.857 PRIMER_INTERNAL_16_GC_PERCENT=38.095 PRIMER_LEFT_16_SELF_ANY=8.00 PRIMER_RIGHT_16_SELF_ANY=2.00 PRIMER_INTERNAL_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_INTERNAL_16_SELF_END=4.00 PRIMER_LEFT_16_END_STABILITY=6.7000 PRIMER_RIGHT_16_END_STABILITY=8.5000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PAIR_16_PRODUCT_SIZE=182 PRIMER_PAIR_17_PENALTY=3.707313 PRIMER_LEFT_17_PENALTY=2.290346 PRIMER_RIGHT_17_PENALTY=1.416967 PRIMER_INTERNAL_17_PENALTY=1.054785 PRIMER_LEFT_17_SEQUENCE=ttccctccacattctctttg PRIMER_RIGHT_17_SEQUENCE=tgatgaaggaaagcaagaagc PRIMER_INTERNAL_17_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_17=306,20 PRIMER_RIGHT_17=525,21 PRIMER_INTERNAL_17=332,21 PRIMER_LEFT_17_TM=57.710 PRIMER_RIGHT_17_TM=59.583 PRIMER_INTERNAL_17_TM=59.945 PRIMER_LEFT_17_GC_PERCENT=45.000 PRIMER_RIGHT_17_GC_PERCENT=42.857 PRIMER_INTERNAL_17_GC_PERCENT=38.095 PRIMER_LEFT_17_SELF_ANY=2.00 PRIMER_RIGHT_17_SELF_ANY=2.00 PRIMER_INTERNAL_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_INTERNAL_17_SELF_END=4.00 PRIMER_LEFT_17_END_STABILITY=7.3000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PAIR_17_PRODUCT_SIZE=220 PRIMER_PAIR_18_PENALTY=3.755432 PRIMER_LEFT_18_PENALTY=0.727304 PRIMER_RIGHT_18_PENALTY=3.028128 PRIMER_INTERNAL_18_PENALTY=3.879062 PRIMER_LEFT_18_SEQUENCE=gctttccttcatcagccatt PRIMER_RIGHT_18_SEQUENCE=cacacccttttacacatttggat PRIMER_INTERNAL_18_SEQUENCE=ttgttcattctgtttctggattg PRIMER_LEFT_18=512,20 PRIMER_RIGHT_18=656,23 PRIMER_INTERNAL_18=593,23 PRIMER_LEFT_18_TM=59.273 PRIMER_RIGHT_18_TM=60.028 PRIMER_INTERNAL_18_TM=59.121 PRIMER_LEFT_18_GC_PERCENT=45.000 PRIMER_RIGHT_18_GC_PERCENT=39.130 PRIMER_INTERNAL_18_GC_PERCENT=34.783 PRIMER_LEFT_18_SELF_ANY=3.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_INTERNAL_18_SELF_ANY=3.00 PRIMER_LEFT_18_SELF_END=1.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_INTERNAL_18_SELF_END=0.00 PRIMER_LEFT_18_END_STABILITY=8.4000 PRIMER_RIGHT_18_END_STABILITY=8.1000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PAIR_18_PRODUCT_SIZE=145 PRIMER_PAIR_19_PENALTY=3.784911 PRIMER_LEFT_19_PENALTY=2.367944 PRIMER_RIGHT_19_PENALTY=1.416967 PRIMER_INTERNAL_19_PENALTY=1.054785 PRIMER_LEFT_19_SEQUENCE=tttgttccctccacattctct PRIMER_RIGHT_19_SEQUENCE=gctgatgaaggaaagcaagaa PRIMER_INTERNAL_19_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_19=302,21 PRIMER_RIGHT_19=527,21 PRIMER_INTERNAL_19=332,21 PRIMER_LEFT_19_TM=58.632 PRIMER_RIGHT_19_TM=59.583 PRIMER_INTERNAL_19_TM=59.945 PRIMER_LEFT_19_GC_PERCENT=42.857 PRIMER_RIGHT_19_GC_PERCENT=42.857 PRIMER_INTERNAL_19_GC_PERCENT=38.095 PRIMER_LEFT_19_SELF_ANY=3.00 PRIMER_RIGHT_19_SELF_ANY=3.00 PRIMER_INTERNAL_19_SELF_ANY=4.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_INTERNAL_19_SELF_END=4.00 PRIMER_LEFT_19_END_STABILITY=6.4000 PRIMER_RIGHT_19_END_STABILITY=7.0000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PAIR_19_PRODUCT_SIZE=226 PRIMER_PAIR_20_PENALTY=3.830133 PRIMER_LEFT_20_PENALTY=1.806577 PRIMER_RIGHT_20_PENALTY=2.023556 PRIMER_INTERNAL_20_PENALTY=5.848837 PRIMER_LEFT_20_SEQUENCE=cgattctccccggtatcaa PRIMER_RIGHT_20_SEQUENCE=aaagagaatgtggagggaacaa PRIMER_INTERNAL_20_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_20=64,19 PRIMER_RIGHT_20=324,22 PRIMER_INTERNAL_20=273,24 PRIMER_LEFT_20_TM=60.807 PRIMER_RIGHT_20_TM=59.976 PRIMER_INTERNAL_20_TM=58.151 PRIMER_LEFT_20_GC_PERCENT=52.632 PRIMER_RIGHT_20_GC_PERCENT=40.909 PRIMER_INTERNAL_20_GC_PERCENT=37.500 PRIMER_LEFT_20_SELF_ANY=4.00 PRIMER_RIGHT_20_SELF_ANY=3.00 PRIMER_INTERNAL_20_SELF_ANY=4.00 PRIMER_LEFT_20_SELF_END=1.00 PRIMER_RIGHT_20_SELF_END=0.00 PRIMER_INTERNAL_20_SELF_END=1.00 PRIMER_LEFT_20_END_STABILITY=6.9000 PRIMER_RIGHT_20_END_STABILITY=7.0000 PRIMER_PAIR_20_COMPL_ANY=6.00 PRIMER_PAIR_20_COMPL_END=1.00 PRIMER_PAIR_20_PRODUCT_SIZE=261 PRIMER_PAIR_21_PENALTY=4.087459 PRIMER_LEFT_21_PENALTY=1.806597 PRIMER_RIGHT_21_PENALTY=2.280862 PRIMER_INTERNAL_21_PENALTY=1.344173 PRIMER_LEFT_21_SEQUENCE=agattcgattctccccggt PRIMER_RIGHT_21_SEQUENCE=gacatgcatgattggatacgtt PRIMER_INTERNAL_21_SEQUENCE=tccctccacattctctttgaa PRIMER_LEFT_21=59,19 PRIMER_RIGHT_21=356,22 PRIMER_INTERNAL_21=307,21 PRIMER_LEFT_21_TM=60.807 PRIMER_RIGHT_21_TM=59.719 PRIMER_INTERNAL_21_TM=59.656 PRIMER_LEFT_21_GC_PERCENT=52.632 PRIMER_RIGHT_21_GC_PERCENT=40.909 PRIMER_INTERNAL_21_GC_PERCENT=42.857 PRIMER_LEFT_21_SELF_ANY=8.00 PRIMER_RIGHT_21_SELF_ANY=8.00 PRIMER_INTERNAL_21_SELF_ANY=3.00 PRIMER_LEFT_21_SELF_END=2.00 PRIMER_RIGHT_21_SELF_END=2.00 PRIMER_INTERNAL_21_SELF_END=3.00 PRIMER_LEFT_21_END_STABILITY=11.1000 PRIMER_RIGHT_21_END_STABILITY=8.1000 PRIMER_PAIR_21_COMPL_ANY=3.00 PRIMER_PAIR_21_COMPL_END=0.00 PRIMER_PAIR_21_PRODUCT_SIZE=298 PRIMER_PAIR_22_PENALTY=4.106176 PRIMER_LEFT_22_PENALTY=0.744257 PRIMER_RIGHT_22_PENALTY=3.361920 PRIMER_INTERNAL_22_PENALTY=3.879062 PRIMER_LEFT_22_SEQUENCE=tgctttccttcatcagccat PRIMER_RIGHT_22_SEQUENCE=cacccttttacacatttggattc PRIMER_INTERNAL_22_SEQUENCE=ttgttcattctgtttctggattg PRIMER_LEFT_22=511,20 PRIMER_RIGHT_22=654,23 PRIMER_INTERNAL_22=593,23 PRIMER_LEFT_22_TM=60.744 PRIMER_RIGHT_22_TM=59.638 PRIMER_INTERNAL_22_TM=59.121 PRIMER_LEFT_22_GC_PERCENT=45.000 PRIMER_RIGHT_22_GC_PERCENT=39.130 PRIMER_INTERNAL_22_GC_PERCENT=34.783 PRIMER_LEFT_22_SELF_ANY=3.00 PRIMER_RIGHT_22_SELF_ANY=4.00 PRIMER_INTERNAL_22_SELF_ANY=3.00 PRIMER_LEFT_22_SELF_END=2.00 PRIMER_RIGHT_22_SELF_END=3.00 PRIMER_INTERNAL_22_SELF_END=0.00 PRIMER_LEFT_22_END_STABILITY=9.6000 PRIMER_RIGHT_22_END_STABILITY=6.6000 PRIMER_PAIR_22_COMPL_ANY=3.00 PRIMER_PAIR_22_COMPL_END=0.00 PRIMER_PAIR_22_PRODUCT_SIZE=144 PRIMER_PAIR_23_PENALTY=4.194881 PRIMER_LEFT_23_PENALTY=2.367944 PRIMER_RIGHT_23_PENALTY=1.826937 PRIMER_INTERNAL_23_PENALTY=1.054785 PRIMER_LEFT_23_SEQUENCE=ctttgttccctccacattctc PRIMER_RIGHT_23_SEQUENCE=gctgatgaaggaaagcaaga PRIMER_INTERNAL_23_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_23=301,21 PRIMER_RIGHT_23=527,20 PRIMER_INTERNAL_23=332,21 PRIMER_LEFT_23_TM=58.632 PRIMER_RIGHT_23_TM=58.173 PRIMER_INTERNAL_23_TM=59.945 PRIMER_LEFT_23_GC_PERCENT=47.619 PRIMER_RIGHT_23_GC_PERCENT=45.000 PRIMER_INTERNAL_23_GC_PERCENT=38.095 PRIMER_LEFT_23_SELF_ANY=3.00 PRIMER_RIGHT_23_SELF_ANY=3.00 PRIMER_INTERNAL_23_SELF_ANY=4.00 PRIMER_LEFT_23_SELF_END=0.00 PRIMER_RIGHT_23_SELF_END=0.00 PRIMER_INTERNAL_23_SELF_END=4.00 PRIMER_LEFT_23_END_STABILITY=6.7000 PRIMER_RIGHT_23_END_STABILITY=7.0000 PRIMER_PAIR_23_COMPL_ANY=6.00 PRIMER_PAIR_23_COMPL_END=3.00 PRIMER_PAIR_23_PRODUCT_SIZE=227 PRIMER_PAIR_24_PENALTY=4.216158 PRIMER_LEFT_24_PENALTY=3.028128 PRIMER_RIGHT_24_PENALTY=1.188029 PRIMER_INTERNAL_24_PENALTY=4.438896 PRIMER_LEFT_24_SEQUENCE=ccaaatgtgtaaaagggtgtgat PRIMER_RIGHT_24_SEQUENCE=atgggaacggaacgttgat PRIMER_INTERNAL_24_SEQUENCE=gcaatcaaagattgttcttaccaa PRIMER_LEFT_24=636,23 PRIMER_RIGHT_24=836,19 PRIMER_INTERNAL_24=721,24 PRIMER_LEFT_24_TM=60.028 PRIMER_RIGHT_24_TM=60.188 PRIMER_INTERNAL_24_TM=59.561 PRIMER_LEFT_24_GC_PERCENT=39.130 PRIMER_RIGHT_24_GC_PERCENT=47.368 PRIMER_INTERNAL_24_GC_PERCENT=33.333 PRIMER_LEFT_24_SELF_ANY=4.00 PRIMER_RIGHT_24_SELF_ANY=6.00 PRIMER_INTERNAL_24_SELF_ANY=7.00 PRIMER_LEFT_24_SELF_END=2.00 PRIMER_RIGHT_24_SELF_END=2.00 PRIMER_INTERNAL_24_SELF_END=3.00 PRIMER_LEFT_24_END_STABILITY=6.3000 PRIMER_RIGHT_24_END_STABILITY=6.9000 PRIMER_PAIR_24_COMPL_ANY=3.00 PRIMER_PAIR_24_COMPL_END=2.00 PRIMER_PAIR_24_PRODUCT_SIZE=201 = SEQUENCE_ID=Must obtain same result as test before SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=25 PRIMER_TASK=generic PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 PRIMER_MUST_MATCH_THREE_PRIME=nnnnn PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=nnnnn PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=nnnnn PRIMER_LEFT_EXPLAIN=considered 8496, GC content failed 704, low tm 5558, high tm 529, high end compl 10, long poly-x seq 92, ok 1603 PRIMER_RIGHT_EXPLAIN=considered 8494, GC content failed 723, low tm 5642, high tm 406, high end compl 10, long poly-x seq 92, ok 1621 PRIMER_INTERNAL_EXPLAIN=considered 9271, GC content failed 725, low tm 6079, high tm 542, long poly-x seq 92, ok 1833 PRIMER_PAIR_EXPLAIN=considered 2657, unacceptable product size 2620, high end compl 1, primer in pair overlaps a primer in a better pair 2423, ok 36 PRIMER_LEFT_NUM_RETURNED=25 PRIMER_RIGHT_NUM_RETURNED=25 PRIMER_INTERNAL_NUM_RETURNED=25 PRIMER_PAIR_NUM_RETURNED=25 PRIMER_PAIR_0_PENALTY=1.171882 PRIMER_LEFT_0_PENALTY=0.117097 PRIMER_RIGHT_0_PENALTY=1.054785 PRIMER_INTERNAL_0_PENALTY=1.344173 PRIMER_LEFT_0_SEQUENCE=attcgattctccccggtatc PRIMER_RIGHT_0_SEQUENCE=tgcatgattggatacgtttga PRIMER_INTERNAL_0_SEQUENCE=tccctccacattctctttgaa PRIMER_LEFT_0=61,20 PRIMER_RIGHT_0=352,21 PRIMER_INTERNAL_0=307,21 PRIMER_LEFT_0_TM=60.117 PRIMER_RIGHT_0_TM=59.945 PRIMER_INTERNAL_0_TM=59.656 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_INTERNAL_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.3000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=292 PRIMER_PAIR_1_PENALTY=1.389004 PRIMER_LEFT_1_PENALTY=1.344173 PRIMER_RIGHT_1_PENALTY=0.044832 PRIMER_INTERNAL_1_PENALTY=1.054785 PRIMER_LEFT_1_SEQUENCE=tccctccacattctctttgaa PRIMER_RIGHT_1_SEQUENCE=ggctgatgaaggaaagcaag PRIMER_INTERNAL_1_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_1=307,21 PRIMER_RIGHT_1=528,20 PRIMER_INTERNAL_1=332,21 PRIMER_LEFT_1_TM=59.656 PRIMER_RIGHT_1_TM=59.955 PRIMER_INTERNAL_1_TM=59.945 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=222 PRIMER_PAIR_2_PENALTY=1.461269 PRIMER_LEFT_2_PENALTY=0.117097 PRIMER_RIGHT_2_PENALTY=1.344173 PRIMER_INTERNAL_2_PENALTY=5.848837 PRIMER_LEFT_2_SEQUENCE=gattcgattctccccggtat PRIMER_RIGHT_2_SEQUENCE=tcaaagagaatgtggagggaa PRIMER_INTERNAL_2_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_2=60,20 PRIMER_RIGHT_2=326,21 PRIMER_INTERNAL_2=273,24 PRIMER_LEFT_2_TM=60.117 PRIMER_RIGHT_2_TM=59.656 PRIMER_INTERNAL_2_TM=58.151 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.8000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=267 PRIMER_PAIR_3_PENALTY=2.071477 PRIMER_LEFT_3_PENALTY=1.344173 PRIMER_RIGHT_3_PENALTY=0.727304 PRIMER_INTERNAL_3_PENALTY=1.054785 PRIMER_LEFT_3_SEQUENCE=ttccctccacattctctttga PRIMER_RIGHT_3_SEQUENCE=aatggctgatgaaggaaagc PRIMER_INTERNAL_3_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_3=306,21 PRIMER_RIGHT_3=531,20 PRIMER_INTERNAL_3=332,21 PRIMER_LEFT_3_TM=59.656 PRIMER_RIGHT_3_TM=59.273 PRIMER_INTERNAL_3_TM=59.945 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=226 PRIMER_PAIR_4_PENALTY=2.122810 PRIMER_LEFT_4_PENALTY=1.058858 PRIMER_RIGHT_4_PENALTY=1.063952 PRIMER_INTERNAL_4_PENALTY=4.596606 PRIMER_LEFT_4_SEQUENCE=cccatgtgaatgtattggagg PRIMER_RIGHT_4_SEQUENCE=tcaacagttgtcaaacttgcg PRIMER_INTERNAL_4_SEQUENCE=ggagacgattatgatttaattgca PRIMER_LEFT_4=832,21 PRIMER_RIGHT_4=948,21 PRIMER_INTERNAL_4=893,24 PRIMER_LEFT_4_TM=60.059 PRIMER_RIGHT_4_TM=59.936 PRIMER_INTERNAL_4_TM=59.403 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=10.5000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=117 PRIMER_PAIR_5_PENALTY=2.131001 PRIMER_LEFT_5_PENALTY=0.786828 PRIMER_RIGHT_5_PENALTY=1.344173 PRIMER_INTERNAL_5_PENALTY=5.848837 PRIMER_LEFT_5_SEQUENCE=agattcgattctccccggta PRIMER_RIGHT_5_SEQUENCE=ttcaaagagaatgtggaggga PRIMER_INTERNAL_5_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_5=59,20 PRIMER_RIGHT_5=327,21 PRIMER_INTERNAL_5=273,24 PRIMER_LEFT_5_TM=60.787 PRIMER_RIGHT_5_TM=59.656 PRIMER_INTERNAL_5_TM=58.151 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=42.857 PRIMER_INTERNAL_5_GC_PERCENT=37.500 PRIMER_LEFT_5_SELF_ANY=8.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_INTERNAL_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_INTERNAL_5_SELF_END=1.00 PRIMER_LEFT_5_END_STABILITY=8.9000 PRIMER_RIGHT_5_END_STABILITY=9.4000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=269 PRIMER_PAIR_6_PENALTY=2.227098 PRIMER_LEFT_6_PENALTY=1.482841 PRIMER_RIGHT_6_PENALTY=0.744257 PRIMER_INTERNAL_6_PENALTY=3.156485 PRIMER_LEFT_6_SEQUENCE=tccaatcatgcatgtctgttc PRIMER_RIGHT_6_SEQUENCE=atggctgatgaaggaaagca PRIMER_INTERNAL_6_SEQUENCE=tgatcataacagcaaaagcatg PRIMER_LEFT_6=341,21 PRIMER_RIGHT_6=530,20 PRIMER_INTERNAL_6=362,22 PRIMER_LEFT_6_TM=59.517 PRIMER_RIGHT_6_TM=60.744 PRIMER_INTERNAL_6_TM=58.844 PRIMER_LEFT_6_GC_PERCENT=42.857 PRIMER_RIGHT_6_GC_PERCENT=45.000 PRIMER_INTERNAL_6_GC_PERCENT=36.364 PRIMER_LEFT_6_SELF_ANY=8.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_INTERNAL_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_INTERNAL_6_SELF_END=4.00 PRIMER_LEFT_6_END_STABILITY=6.7000 PRIMER_RIGHT_6_END_STABILITY=8.5000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=190 PRIMER_PAIR_7_PENALTY=2.282031 PRIMER_LEFT_7_PENALTY=0.044832 PRIMER_RIGHT_7_PENALTY=2.237199 PRIMER_INTERNAL_7_PENALTY=3.879062 PRIMER_LEFT_7_SEQUENCE=cttgctttccttcatcagcc PRIMER_RIGHT_7_SEQUENCE=tcacacccttttacacatttgg PRIMER_INTERNAL_7_SEQUENCE=ttgttcattctgtttctggattg PRIMER_LEFT_7=509,20 PRIMER_RIGHT_7=657,22 PRIMER_INTERNAL_7=593,23 PRIMER_LEFT_7_TM=59.955 PRIMER_RIGHT_7_TM=59.763 PRIMER_INTERNAL_7_TM=59.121 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=40.909 PRIMER_INTERNAL_7_GC_PERCENT=34.783 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_INTERNAL_7_SELF_ANY=3.00 PRIMER_LEFT_7_SELF_END=1.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_INTERNAL_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=8.8000 PRIMER_PAIR_7_COMPL_ANY=2.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PAIR_7_PRODUCT_SIZE=149 PRIMER_PAIR_8_PENALTY=2.676544 PRIMER_LEFT_8_PENALTY=0.833045 PRIMER_RIGHT_8_PENALTY=1.843499 PRIMER_INTERNAL_8_PENALTY=5.848837 PRIMER_LEFT_8_SEQUENCE=tctcagattcgattctcccc PRIMER_RIGHT_8_SEQUENCE=tttcaaagagaatgtggaggg PRIMER_INTERNAL_8_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_8=55,20 PRIMER_RIGHT_8=328,21 PRIMER_INTERNAL_8=273,24 PRIMER_LEFT_8_TM=59.167 PRIMER_RIGHT_8_TM=59.157 PRIMER_INTERNAL_8_TM=58.151 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=42.857 PRIMER_INTERNAL_8_GC_PERCENT=37.500 PRIMER_LEFT_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_INTERNAL_8_SELF_ANY=4.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_INTERNAL_8_SELF_END=1.00 PRIMER_LEFT_8_END_STABILITY=10.9000 PRIMER_RIGHT_8_END_STABILITY=9.4000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PAIR_8_PRODUCT_SIZE=274 PRIMER_PAIR_9_PENALTY=3.041124 PRIMER_LEFT_9_PENALTY=1.029407 PRIMER_RIGHT_9_PENALTY=2.011716 PRIMER_INTERNAL_9_PENALTY=1.344173 PRIMER_LEFT_9_SEQUENCE=ggtctcagattcgattctccc PRIMER_RIGHT_9_SEQUENCE=tgcatgattggatacgtttg PRIMER_INTERNAL_9_SEQUENCE=tccctccacattctctttgaa PRIMER_LEFT_9=53,21 PRIMER_RIGHT_9=352,20 PRIMER_INTERNAL_9=307,21 PRIMER_LEFT_9_TM=60.029 PRIMER_RIGHT_9_TM=57.988 PRIMER_INTERNAL_9_TM=59.656 PRIMER_LEFT_9_GC_PERCENT=52.381 PRIMER_RIGHT_9_GC_PERCENT=40.000 PRIMER_INTERNAL_9_GC_PERCENT=42.857 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_INTERNAL_9_SELF_ANY=3.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_INTERNAL_9_SELF_END=3.00 PRIMER_LEFT_9_END_STABILITY=9.4000 PRIMER_RIGHT_9_END_STABILITY=7.0000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=300 PRIMER_PAIR_10_PENALTY=3.057112 PRIMER_LEFT_10_PENALTY=1.033556 PRIMER_RIGHT_10_PENALTY=2.023556 PRIMER_INTERNAL_10_PENALTY=5.848837 PRIMER_LEFT_10_SEQUENCE=cgattctccccggtatcaat PRIMER_RIGHT_10_SEQUENCE=aagagaatgtggagggaacaaa PRIMER_INTERNAL_10_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_10=64,20 PRIMER_RIGHT_10=323,22 PRIMER_INTERNAL_10=273,24 PRIMER_LEFT_10_TM=61.034 PRIMER_RIGHT_10_TM=59.976 PRIMER_INTERNAL_10_TM=58.151 PRIMER_LEFT_10_GC_PERCENT=50.000 PRIMER_RIGHT_10_GC_PERCENT=40.909 PRIMER_INTERNAL_10_GC_PERCENT=37.500 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=3.00 PRIMER_INTERNAL_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_INTERNAL_10_SELF_END=1.00 PRIMER_LEFT_10_END_STABILITY=6.9000 PRIMER_RIGHT_10_END_STABILITY=7.0000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=260 PRIMER_PAIR_11_PENALTY=3.140307 PRIMER_LEFT_11_PENALTY=2.023556 PRIMER_RIGHT_11_PENALTY=1.116751 PRIMER_INTERNAL_11_PENALTY=1.054785 PRIMER_LEFT_11_SEQUENCE=ttgttccctccacattctcttt PRIMER_RIGHT_11_SEQUENCE=tgaaggaaagcaagaagcaaa PRIMER_INTERNAL_11_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_11=303,22 PRIMER_RIGHT_11=522,21 PRIMER_INTERNAL_11=332,21 PRIMER_LEFT_11_TM=59.976 PRIMER_RIGHT_11_TM=60.117 PRIMER_INTERNAL_11_TM=59.945 PRIMER_LEFT_11_GC_PERCENT=40.909 PRIMER_RIGHT_11_GC_PERCENT=38.095 PRIMER_INTERNAL_11_GC_PERCENT=38.095 PRIMER_LEFT_11_SELF_ANY=3.00 PRIMER_RIGHT_11_SELF_ANY=2.00 PRIMER_INTERNAL_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_INTERNAL_11_SELF_END=4.00 PRIMER_LEFT_11_END_STABILITY=7.0000 PRIMER_RIGHT_11_END_STABILITY=8.8000 PRIMER_PAIR_11_COMPL_ANY=6.00 PRIMER_PAIR_11_COMPL_END=3.00 PRIMER_PAIR_11_PRODUCT_SIZE=220 PRIMER_PAIR_12_PENALTY=3.252729 PRIMER_LEFT_12_PENALTY=2.023556 PRIMER_RIGHT_12_PENALTY=1.229173 PRIMER_INTERNAL_12_PENALTY=1.054785 PRIMER_LEFT_12_SEQUENCE=tttgttccctccacattctctt PRIMER_RIGHT_12_SEQUENCE=tgaaggaaagcaagaagcaa PRIMER_INTERNAL_12_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_12=302,22 PRIMER_RIGHT_12=522,20 PRIMER_INTERNAL_12=332,21 PRIMER_LEFT_12_TM=59.976 PRIMER_RIGHT_12_TM=58.771 PRIMER_INTERNAL_12_TM=59.945 PRIMER_LEFT_12_GC_PERCENT=40.909 PRIMER_RIGHT_12_GC_PERCENT=40.000 PRIMER_INTERNAL_12_GC_PERCENT=38.095 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=2.00 PRIMER_INTERNAL_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_INTERNAL_12_SELF_END=4.00 PRIMER_LEFT_12_END_STABILITY=6.7000 PRIMER_RIGHT_12_END_STABILITY=8.5000 PRIMER_PAIR_12_COMPL_ANY=6.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PAIR_12_PRODUCT_SIZE=221 PRIMER_PAIR_13_PENALTY=3.299314 PRIMER_LEFT_13_PENALTY=1.188029 PRIMER_RIGHT_13_PENALTY=2.111285 PRIMER_INTERNAL_13_PENALTY=3.306406 PRIMER_LEFT_13_SEQUENCE=atcaacgttccgttcccat PRIMER_RIGHT_13_SEQUENCE=caacagttgtcaaacttgcgt PRIMER_INTERNAL_13_SEQUENCE=ttggaggtgaatttctaggaca PRIMER_LEFT_13=818,19 PRIMER_RIGHT_13=947,21 PRIMER_INTERNAL_13=846,22 PRIMER_LEFT_13_TM=60.188 PRIMER_RIGHT_13_TM=58.889 PRIMER_INTERNAL_13_TM=58.694 PRIMER_LEFT_13_GC_PERCENT=47.368 PRIMER_RIGHT_13_GC_PERCENT=42.857 PRIMER_INTERNAL_13_GC_PERCENT=40.909 PRIMER_LEFT_13_SELF_ANY=6.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_INTERNAL_13_SELF_ANY=5.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_INTERNAL_13_SELF_END=2.00 PRIMER_LEFT_13_END_STABILITY=9.6000 PRIMER_RIGHT_13_END_STABILITY=9.9000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=0.00 PRIMER_PAIR_13_PRODUCT_SIZE=130 PRIMER_PAIR_14_PENALTY=3.404939 PRIMER_LEFT_14_PENALTY=2.237199 PRIMER_RIGHT_14_PENALTY=1.167740 PRIMER_INTERNAL_14_PENALTY=4.438896 PRIMER_LEFT_14_SEQUENCE=ccaaatgtgtaaaagggtgtga PRIMER_RIGHT_14_SEQUENCE=cattcacatgggaacggaac PRIMER_INTERNAL_14_SEQUENCE=gcaatcaaagattgttcttaccaa PRIMER_LEFT_14=636,22 PRIMER_RIGHT_14=843,20 PRIMER_INTERNAL_14=721,24 PRIMER_LEFT_14_TM=59.763 PRIMER_RIGHT_14_TM=61.168 PRIMER_INTERNAL_14_TM=59.561 PRIMER_LEFT_14_GC_PERCENT=40.909 PRIMER_RIGHT_14_GC_PERCENT=50.000 PRIMER_INTERNAL_14_GC_PERCENT=33.333 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=8.00 PRIMER_INTERNAL_14_SELF_ANY=7.00 PRIMER_LEFT_14_SELF_END=1.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_INTERNAL_14_SELF_END=3.00 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=7.9000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=0.00 PRIMER_PAIR_14_PRODUCT_SIZE=208 PRIMER_PAIR_15_PENALTY=3.404939 PRIMER_LEFT_15_PENALTY=2.237199 PRIMER_RIGHT_15_PENALTY=1.167740 PRIMER_INTERNAL_15_PENALTY=4.438896 PRIMER_LEFT_15_SEQUENCE=tccaaatgtgtaaaagggtgtg PRIMER_RIGHT_15_SEQUENCE=acattcacatgggaacggaa PRIMER_INTERNAL_15_SEQUENCE=gcaatcaaagattgttcttaccaa PRIMER_LEFT_15=635,22 PRIMER_RIGHT_15=844,20 PRIMER_INTERNAL_15=721,24 PRIMER_LEFT_15_TM=59.763 PRIMER_RIGHT_15_TM=61.168 PRIMER_INTERNAL_15_TM=59.561 PRIMER_LEFT_15_GC_PERCENT=40.909 PRIMER_RIGHT_15_GC_PERCENT=45.000 PRIMER_INTERNAL_15_GC_PERCENT=33.333 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=8.00 PRIMER_INTERNAL_15_SELF_ANY=7.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_INTERNAL_15_SELF_END=3.00 PRIMER_LEFT_15_END_STABILITY=6.4000 PRIMER_RIGHT_15_END_STABILITY=10.2000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=210 PRIMER_PAIR_16_PENALTY=3.695091 PRIMER_LEFT_16_PENALTY=2.278124 PRIMER_RIGHT_16_PENALTY=1.416967 PRIMER_INTERNAL_16_PENALTY=3.541086 PRIMER_LEFT_16_SEQUENCE=caatcatgcatgtctgttctga PRIMER_RIGHT_16_SEQUENCE=gatgaaggaaagcaagaagca PRIMER_INTERNAL_16_SEQUENCE=tcataacagcaaaagcatgtg PRIMER_LEFT_16=343,22 PRIMER_RIGHT_16=524,21 PRIMER_INTERNAL_16=365,21 PRIMER_LEFT_16_TM=59.722 PRIMER_RIGHT_16_TM=59.583 PRIMER_INTERNAL_16_TM=57.459 PRIMER_LEFT_16_GC_PERCENT=40.909 PRIMER_RIGHT_16_GC_PERCENT=42.857 PRIMER_INTERNAL_16_GC_PERCENT=38.095 PRIMER_LEFT_16_SELF_ANY=8.00 PRIMER_RIGHT_16_SELF_ANY=2.00 PRIMER_INTERNAL_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_INTERNAL_16_SELF_END=4.00 PRIMER_LEFT_16_END_STABILITY=6.7000 PRIMER_RIGHT_16_END_STABILITY=8.5000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PAIR_16_PRODUCT_SIZE=182 PRIMER_PAIR_17_PENALTY=3.707313 PRIMER_LEFT_17_PENALTY=2.290346 PRIMER_RIGHT_17_PENALTY=1.416967 PRIMER_INTERNAL_17_PENALTY=1.054785 PRIMER_LEFT_17_SEQUENCE=ttccctccacattctctttg PRIMER_RIGHT_17_SEQUENCE=tgatgaaggaaagcaagaagc PRIMER_INTERNAL_17_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_17=306,20 PRIMER_RIGHT_17=525,21 PRIMER_INTERNAL_17=332,21 PRIMER_LEFT_17_TM=57.710 PRIMER_RIGHT_17_TM=59.583 PRIMER_INTERNAL_17_TM=59.945 PRIMER_LEFT_17_GC_PERCENT=45.000 PRIMER_RIGHT_17_GC_PERCENT=42.857 PRIMER_INTERNAL_17_GC_PERCENT=38.095 PRIMER_LEFT_17_SELF_ANY=2.00 PRIMER_RIGHT_17_SELF_ANY=2.00 PRIMER_INTERNAL_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_INTERNAL_17_SELF_END=4.00 PRIMER_LEFT_17_END_STABILITY=7.3000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PAIR_17_PRODUCT_SIZE=220 PRIMER_PAIR_18_PENALTY=3.755432 PRIMER_LEFT_18_PENALTY=0.727304 PRIMER_RIGHT_18_PENALTY=3.028128 PRIMER_INTERNAL_18_PENALTY=3.879062 PRIMER_LEFT_18_SEQUENCE=gctttccttcatcagccatt PRIMER_RIGHT_18_SEQUENCE=cacacccttttacacatttggat PRIMER_INTERNAL_18_SEQUENCE=ttgttcattctgtttctggattg PRIMER_LEFT_18=512,20 PRIMER_RIGHT_18=656,23 PRIMER_INTERNAL_18=593,23 PRIMER_LEFT_18_TM=59.273 PRIMER_RIGHT_18_TM=60.028 PRIMER_INTERNAL_18_TM=59.121 PRIMER_LEFT_18_GC_PERCENT=45.000 PRIMER_RIGHT_18_GC_PERCENT=39.130 PRIMER_INTERNAL_18_GC_PERCENT=34.783 PRIMER_LEFT_18_SELF_ANY=3.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_INTERNAL_18_SELF_ANY=3.00 PRIMER_LEFT_18_SELF_END=1.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_INTERNAL_18_SELF_END=0.00 PRIMER_LEFT_18_END_STABILITY=8.4000 PRIMER_RIGHT_18_END_STABILITY=8.1000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PAIR_18_PRODUCT_SIZE=145 PRIMER_PAIR_19_PENALTY=3.784911 PRIMER_LEFT_19_PENALTY=2.367944 PRIMER_RIGHT_19_PENALTY=1.416967 PRIMER_INTERNAL_19_PENALTY=1.054785 PRIMER_LEFT_19_SEQUENCE=tttgttccctccacattctct PRIMER_RIGHT_19_SEQUENCE=gctgatgaaggaaagcaagaa PRIMER_INTERNAL_19_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_19=302,21 PRIMER_RIGHT_19=527,21 PRIMER_INTERNAL_19=332,21 PRIMER_LEFT_19_TM=58.632 PRIMER_RIGHT_19_TM=59.583 PRIMER_INTERNAL_19_TM=59.945 PRIMER_LEFT_19_GC_PERCENT=42.857 PRIMER_RIGHT_19_GC_PERCENT=42.857 PRIMER_INTERNAL_19_GC_PERCENT=38.095 PRIMER_LEFT_19_SELF_ANY=3.00 PRIMER_RIGHT_19_SELF_ANY=3.00 PRIMER_INTERNAL_19_SELF_ANY=4.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_INTERNAL_19_SELF_END=4.00 PRIMER_LEFT_19_END_STABILITY=6.4000 PRIMER_RIGHT_19_END_STABILITY=7.0000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PAIR_19_PRODUCT_SIZE=226 PRIMER_PAIR_20_PENALTY=3.830133 PRIMER_LEFT_20_PENALTY=1.806577 PRIMER_RIGHT_20_PENALTY=2.023556 PRIMER_INTERNAL_20_PENALTY=5.848837 PRIMER_LEFT_20_SEQUENCE=cgattctccccggtatcaa PRIMER_RIGHT_20_SEQUENCE=aaagagaatgtggagggaacaa PRIMER_INTERNAL_20_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_20=64,19 PRIMER_RIGHT_20=324,22 PRIMER_INTERNAL_20=273,24 PRIMER_LEFT_20_TM=60.807 PRIMER_RIGHT_20_TM=59.976 PRIMER_INTERNAL_20_TM=58.151 PRIMER_LEFT_20_GC_PERCENT=52.632 PRIMER_RIGHT_20_GC_PERCENT=40.909 PRIMER_INTERNAL_20_GC_PERCENT=37.500 PRIMER_LEFT_20_SELF_ANY=4.00 PRIMER_RIGHT_20_SELF_ANY=3.00 PRIMER_INTERNAL_20_SELF_ANY=4.00 PRIMER_LEFT_20_SELF_END=1.00 PRIMER_RIGHT_20_SELF_END=0.00 PRIMER_INTERNAL_20_SELF_END=1.00 PRIMER_LEFT_20_END_STABILITY=6.9000 PRIMER_RIGHT_20_END_STABILITY=7.0000 PRIMER_PAIR_20_COMPL_ANY=6.00 PRIMER_PAIR_20_COMPL_END=1.00 PRIMER_PAIR_20_PRODUCT_SIZE=261 PRIMER_PAIR_21_PENALTY=4.087459 PRIMER_LEFT_21_PENALTY=1.806597 PRIMER_RIGHT_21_PENALTY=2.280862 PRIMER_INTERNAL_21_PENALTY=1.344173 PRIMER_LEFT_21_SEQUENCE=agattcgattctccccggt PRIMER_RIGHT_21_SEQUENCE=gacatgcatgattggatacgtt PRIMER_INTERNAL_21_SEQUENCE=tccctccacattctctttgaa PRIMER_LEFT_21=59,19 PRIMER_RIGHT_21=356,22 PRIMER_INTERNAL_21=307,21 PRIMER_LEFT_21_TM=60.807 PRIMER_RIGHT_21_TM=59.719 PRIMER_INTERNAL_21_TM=59.656 PRIMER_LEFT_21_GC_PERCENT=52.632 PRIMER_RIGHT_21_GC_PERCENT=40.909 PRIMER_INTERNAL_21_GC_PERCENT=42.857 PRIMER_LEFT_21_SELF_ANY=8.00 PRIMER_RIGHT_21_SELF_ANY=8.00 PRIMER_INTERNAL_21_SELF_ANY=3.00 PRIMER_LEFT_21_SELF_END=2.00 PRIMER_RIGHT_21_SELF_END=2.00 PRIMER_INTERNAL_21_SELF_END=3.00 PRIMER_LEFT_21_END_STABILITY=11.1000 PRIMER_RIGHT_21_END_STABILITY=8.1000 PRIMER_PAIR_21_COMPL_ANY=3.00 PRIMER_PAIR_21_COMPL_END=0.00 PRIMER_PAIR_21_PRODUCT_SIZE=298 PRIMER_PAIR_22_PENALTY=4.106176 PRIMER_LEFT_22_PENALTY=0.744257 PRIMER_RIGHT_22_PENALTY=3.361920 PRIMER_INTERNAL_22_PENALTY=3.879062 PRIMER_LEFT_22_SEQUENCE=tgctttccttcatcagccat PRIMER_RIGHT_22_SEQUENCE=cacccttttacacatttggattc PRIMER_INTERNAL_22_SEQUENCE=ttgttcattctgtttctggattg PRIMER_LEFT_22=511,20 PRIMER_RIGHT_22=654,23 PRIMER_INTERNAL_22=593,23 PRIMER_LEFT_22_TM=60.744 PRIMER_RIGHT_22_TM=59.638 PRIMER_INTERNAL_22_TM=59.121 PRIMER_LEFT_22_GC_PERCENT=45.000 PRIMER_RIGHT_22_GC_PERCENT=39.130 PRIMER_INTERNAL_22_GC_PERCENT=34.783 PRIMER_LEFT_22_SELF_ANY=3.00 PRIMER_RIGHT_22_SELF_ANY=4.00 PRIMER_INTERNAL_22_SELF_ANY=3.00 PRIMER_LEFT_22_SELF_END=2.00 PRIMER_RIGHT_22_SELF_END=3.00 PRIMER_INTERNAL_22_SELF_END=0.00 PRIMER_LEFT_22_END_STABILITY=9.6000 PRIMER_RIGHT_22_END_STABILITY=6.6000 PRIMER_PAIR_22_COMPL_ANY=3.00 PRIMER_PAIR_22_COMPL_END=0.00 PRIMER_PAIR_22_PRODUCT_SIZE=144 PRIMER_PAIR_23_PENALTY=4.194881 PRIMER_LEFT_23_PENALTY=2.367944 PRIMER_RIGHT_23_PENALTY=1.826937 PRIMER_INTERNAL_23_PENALTY=1.054785 PRIMER_LEFT_23_SEQUENCE=ctttgttccctccacattctc PRIMER_RIGHT_23_SEQUENCE=gctgatgaaggaaagcaaga PRIMER_INTERNAL_23_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_23=301,21 PRIMER_RIGHT_23=527,20 PRIMER_INTERNAL_23=332,21 PRIMER_LEFT_23_TM=58.632 PRIMER_RIGHT_23_TM=58.173 PRIMER_INTERNAL_23_TM=59.945 PRIMER_LEFT_23_GC_PERCENT=47.619 PRIMER_RIGHT_23_GC_PERCENT=45.000 PRIMER_INTERNAL_23_GC_PERCENT=38.095 PRIMER_LEFT_23_SELF_ANY=3.00 PRIMER_RIGHT_23_SELF_ANY=3.00 PRIMER_INTERNAL_23_SELF_ANY=4.00 PRIMER_LEFT_23_SELF_END=0.00 PRIMER_RIGHT_23_SELF_END=0.00 PRIMER_INTERNAL_23_SELF_END=4.00 PRIMER_LEFT_23_END_STABILITY=6.7000 PRIMER_RIGHT_23_END_STABILITY=7.0000 PRIMER_PAIR_23_COMPL_ANY=6.00 PRIMER_PAIR_23_COMPL_END=3.00 PRIMER_PAIR_23_PRODUCT_SIZE=227 PRIMER_PAIR_24_PENALTY=4.216158 PRIMER_LEFT_24_PENALTY=3.028128 PRIMER_RIGHT_24_PENALTY=1.188029 PRIMER_INTERNAL_24_PENALTY=4.438896 PRIMER_LEFT_24_SEQUENCE=ccaaatgtgtaaaagggtgtgat PRIMER_RIGHT_24_SEQUENCE=atgggaacggaacgttgat PRIMER_INTERNAL_24_SEQUENCE=gcaatcaaagattgttcttaccaa PRIMER_LEFT_24=636,23 PRIMER_RIGHT_24=836,19 PRIMER_INTERNAL_24=721,24 PRIMER_LEFT_24_TM=60.028 PRIMER_RIGHT_24_TM=60.188 PRIMER_INTERNAL_24_TM=59.561 PRIMER_LEFT_24_GC_PERCENT=39.130 PRIMER_RIGHT_24_GC_PERCENT=47.368 PRIMER_INTERNAL_24_GC_PERCENT=33.333 PRIMER_LEFT_24_SELF_ANY=4.00 PRIMER_RIGHT_24_SELF_ANY=6.00 PRIMER_INTERNAL_24_SELF_ANY=7.00 PRIMER_LEFT_24_SELF_END=2.00 PRIMER_RIGHT_24_SELF_END=2.00 PRIMER_INTERNAL_24_SELF_END=3.00 PRIMER_LEFT_24_END_STABILITY=6.3000 PRIMER_RIGHT_24_END_STABILITY=6.9000 PRIMER_PAIR_24_COMPL_ANY=3.00 PRIMER_PAIR_24_COMPL_END=2.00 PRIMER_PAIR_24_PRODUCT_SIZE=201 = SEQUENCE_ID=Pick probes without G at the 3 and 5 prime end SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=25 PRIMER_TASK=generic PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 PRIMER_MUST_MATCH_THREE_PRIME=nnnnn PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=nnnnh PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=hnnnn PRIMER_LEFT_EXPLAIN=considered 8496, GC content failed 704, low tm 5558, high tm 529, high end compl 10, long poly-x seq 92, ok 1603 PRIMER_RIGHT_EXPLAIN=considered 8494, GC content failed 723, low tm 5642, high tm 406, high end compl 10, long poly-x seq 92, ok 1621 PRIMER_INTERNAL_EXPLAIN=considered 9376, GC content failed 675, low tm 4457, high tm 370, long poly-x seq 75, failed must_match requirements 2500, ok 1299 PRIMER_PAIR_EXPLAIN=considered 2657, unacceptable product size 2620, high end compl 1, primer in pair overlaps a primer in a better pair 2423, ok 36 PRIMER_LEFT_NUM_RETURNED=25 PRIMER_RIGHT_NUM_RETURNED=25 PRIMER_INTERNAL_NUM_RETURNED=25 PRIMER_PAIR_NUM_RETURNED=25 PRIMER_PAIR_0_PENALTY=1.171882 PRIMER_LEFT_0_PENALTY=0.117097 PRIMER_RIGHT_0_PENALTY=1.054785 PRIMER_INTERNAL_0_PENALTY=1.344173 PRIMER_LEFT_0_SEQUENCE=attcgattctccccggtatc PRIMER_RIGHT_0_SEQUENCE=tgcatgattggatacgtttga PRIMER_INTERNAL_0_SEQUENCE=tccctccacattctctttgaa PRIMER_LEFT_0=61,20 PRIMER_RIGHT_0=352,21 PRIMER_INTERNAL_0=307,21 PRIMER_LEFT_0_TM=60.117 PRIMER_RIGHT_0_TM=59.945 PRIMER_INTERNAL_0_TM=59.656 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_INTERNAL_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.3000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=292 PRIMER_PAIR_1_PENALTY=1.389004 PRIMER_LEFT_1_PENALTY=1.344173 PRIMER_RIGHT_1_PENALTY=0.044832 PRIMER_INTERNAL_1_PENALTY=1.054785 PRIMER_LEFT_1_SEQUENCE=tccctccacattctctttgaa PRIMER_RIGHT_1_SEQUENCE=ggctgatgaaggaaagcaag PRIMER_INTERNAL_1_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_1=307,21 PRIMER_RIGHT_1=528,20 PRIMER_INTERNAL_1=332,21 PRIMER_LEFT_1_TM=59.656 PRIMER_RIGHT_1_TM=59.955 PRIMER_INTERNAL_1_TM=59.945 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=222 PRIMER_PAIR_2_PENALTY=1.461269 PRIMER_LEFT_2_PENALTY=0.117097 PRIMER_RIGHT_2_PENALTY=1.344173 PRIMER_INTERNAL_2_PENALTY=5.848837 PRIMER_LEFT_2_SEQUENCE=gattcgattctccccggtat PRIMER_RIGHT_2_SEQUENCE=tcaaagagaatgtggagggaa PRIMER_INTERNAL_2_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_2=60,20 PRIMER_RIGHT_2=326,21 PRIMER_INTERNAL_2=273,24 PRIMER_LEFT_2_TM=60.117 PRIMER_RIGHT_2_TM=59.656 PRIMER_INTERNAL_2_TM=58.151 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.8000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=267 PRIMER_PAIR_3_PENALTY=2.071477 PRIMER_LEFT_3_PENALTY=1.344173 PRIMER_RIGHT_3_PENALTY=0.727304 PRIMER_INTERNAL_3_PENALTY=1.054785 PRIMER_LEFT_3_SEQUENCE=ttccctccacattctctttga PRIMER_RIGHT_3_SEQUENCE=aatggctgatgaaggaaagc PRIMER_INTERNAL_3_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_3=306,21 PRIMER_RIGHT_3=531,20 PRIMER_INTERNAL_3=332,21 PRIMER_LEFT_3_TM=59.656 PRIMER_RIGHT_3_TM=59.273 PRIMER_INTERNAL_3_TM=59.945 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=226 PRIMER_PAIR_4_PENALTY=2.122810 PRIMER_LEFT_4_PENALTY=1.058858 PRIMER_RIGHT_4_PENALTY=1.063952 PRIMER_INTERNAL_4_PENALTY=4.967649 PRIMER_LEFT_4_SEQUENCE=cccatgtgaatgtattggagg PRIMER_RIGHT_4_SEQUENCE=tcaacagttgtcaaacttgcg PRIMER_INTERNAL_4_SEQUENCE=acaacaaaagaaggagacgatt PRIMER_LEFT_4=832,21 PRIMER_RIGHT_4=948,21 PRIMER_INTERNAL_4=881,22 PRIMER_LEFT_4_TM=60.059 PRIMER_RIGHT_4_TM=59.936 PRIMER_INTERNAL_4_TM=57.032 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=36.364 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=10.5000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=117 PRIMER_PAIR_5_PENALTY=2.131001 PRIMER_LEFT_5_PENALTY=0.786828 PRIMER_RIGHT_5_PENALTY=1.344173 PRIMER_INTERNAL_5_PENALTY=5.848837 PRIMER_LEFT_5_SEQUENCE=agattcgattctccccggta PRIMER_RIGHT_5_SEQUENCE=ttcaaagagaatgtggaggga PRIMER_INTERNAL_5_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_5=59,20 PRIMER_RIGHT_5=327,21 PRIMER_INTERNAL_5=273,24 PRIMER_LEFT_5_TM=60.787 PRIMER_RIGHT_5_TM=59.656 PRIMER_INTERNAL_5_TM=58.151 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=42.857 PRIMER_INTERNAL_5_GC_PERCENT=37.500 PRIMER_LEFT_5_SELF_ANY=8.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_INTERNAL_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_INTERNAL_5_SELF_END=1.00 PRIMER_LEFT_5_END_STABILITY=8.9000 PRIMER_RIGHT_5_END_STABILITY=9.4000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=269 PRIMER_PAIR_6_PENALTY=2.227098 PRIMER_LEFT_6_PENALTY=1.482841 PRIMER_RIGHT_6_PENALTY=0.744257 PRIMER_INTERNAL_6_PENALTY=3.334070 PRIMER_LEFT_6_SEQUENCE=tccaatcatgcatgtctgttc PRIMER_RIGHT_6_SEQUENCE=atggctgatgaaggaaagca PRIMER_INTERNAL_6_SEQUENCE=tgatcataacagcaaaagcatgt PRIMER_LEFT_6=341,21 PRIMER_RIGHT_6=530,20 PRIMER_INTERNAL_6=362,23 PRIMER_LEFT_6_TM=59.517 PRIMER_RIGHT_6_TM=60.744 PRIMER_INTERNAL_6_TM=59.666 PRIMER_LEFT_6_GC_PERCENT=42.857 PRIMER_RIGHT_6_GC_PERCENT=45.000 PRIMER_INTERNAL_6_GC_PERCENT=34.783 PRIMER_LEFT_6_SELF_ANY=8.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_INTERNAL_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_INTERNAL_6_SELF_END=2.00 PRIMER_LEFT_6_END_STABILITY=6.7000 PRIMER_RIGHT_6_END_STABILITY=8.5000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=190 PRIMER_PAIR_7_PENALTY=2.282031 PRIMER_LEFT_7_PENALTY=0.044832 PRIMER_RIGHT_7_PENALTY=2.237199 PRIMER_INTERNAL_7_PENALTY=4.087031 PRIMER_LEFT_7_SEQUENCE=cttgctttccttcatcagcc PRIMER_RIGHT_7_SEQUENCE=tcacacccttttacacatttgg PRIMER_INTERNAL_7_SEQUENCE=tcattctgtttctggattgtgttt PRIMER_LEFT_7=509,20 PRIMER_RIGHT_7=657,22 PRIMER_INTERNAL_7=597,24 PRIMER_LEFT_7_TM=59.955 PRIMER_RIGHT_7_TM=59.763 PRIMER_INTERNAL_7_TM=59.913 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=40.909 PRIMER_INTERNAL_7_GC_PERCENT=33.333 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_INTERNAL_7_SELF_ANY=3.00 PRIMER_LEFT_7_SELF_END=1.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_INTERNAL_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=8.8000 PRIMER_PAIR_7_COMPL_ANY=2.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PAIR_7_PRODUCT_SIZE=149 PRIMER_PAIR_8_PENALTY=2.676544 PRIMER_LEFT_8_PENALTY=0.833045 PRIMER_RIGHT_8_PENALTY=1.843499 PRIMER_INTERNAL_8_PENALTY=5.848837 PRIMER_LEFT_8_SEQUENCE=tctcagattcgattctcccc PRIMER_RIGHT_8_SEQUENCE=tttcaaagagaatgtggaggg PRIMER_INTERNAL_8_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_8=55,20 PRIMER_RIGHT_8=328,21 PRIMER_INTERNAL_8=273,24 PRIMER_LEFT_8_TM=59.167 PRIMER_RIGHT_8_TM=59.157 PRIMER_INTERNAL_8_TM=58.151 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=42.857 PRIMER_INTERNAL_8_GC_PERCENT=37.500 PRIMER_LEFT_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_INTERNAL_8_SELF_ANY=4.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_INTERNAL_8_SELF_END=1.00 PRIMER_LEFT_8_END_STABILITY=10.9000 PRIMER_RIGHT_8_END_STABILITY=9.4000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PAIR_8_PRODUCT_SIZE=274 PRIMER_PAIR_9_PENALTY=3.041124 PRIMER_LEFT_9_PENALTY=1.029407 PRIMER_RIGHT_9_PENALTY=2.011716 PRIMER_INTERNAL_9_PENALTY=1.344173 PRIMER_LEFT_9_SEQUENCE=ggtctcagattcgattctccc PRIMER_RIGHT_9_SEQUENCE=tgcatgattggatacgtttg PRIMER_INTERNAL_9_SEQUENCE=tccctccacattctctttgaa PRIMER_LEFT_9=53,21 PRIMER_RIGHT_9=352,20 PRIMER_INTERNAL_9=307,21 PRIMER_LEFT_9_TM=60.029 PRIMER_RIGHT_9_TM=57.988 PRIMER_INTERNAL_9_TM=59.656 PRIMER_LEFT_9_GC_PERCENT=52.381 PRIMER_RIGHT_9_GC_PERCENT=40.000 PRIMER_INTERNAL_9_GC_PERCENT=42.857 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_INTERNAL_9_SELF_ANY=3.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_INTERNAL_9_SELF_END=3.00 PRIMER_LEFT_9_END_STABILITY=9.4000 PRIMER_RIGHT_9_END_STABILITY=7.0000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=300 PRIMER_PAIR_10_PENALTY=3.057112 PRIMER_LEFT_10_PENALTY=1.033556 PRIMER_RIGHT_10_PENALTY=2.023556 PRIMER_INTERNAL_10_PENALTY=5.848837 PRIMER_LEFT_10_SEQUENCE=cgattctccccggtatcaat PRIMER_RIGHT_10_SEQUENCE=aagagaatgtggagggaacaaa PRIMER_INTERNAL_10_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_10=64,20 PRIMER_RIGHT_10=323,22 PRIMER_INTERNAL_10=273,24 PRIMER_LEFT_10_TM=61.034 PRIMER_RIGHT_10_TM=59.976 PRIMER_INTERNAL_10_TM=58.151 PRIMER_LEFT_10_GC_PERCENT=50.000 PRIMER_RIGHT_10_GC_PERCENT=40.909 PRIMER_INTERNAL_10_GC_PERCENT=37.500 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=3.00 PRIMER_INTERNAL_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_INTERNAL_10_SELF_END=1.00 PRIMER_LEFT_10_END_STABILITY=6.9000 PRIMER_RIGHT_10_END_STABILITY=7.0000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=260 PRIMER_PAIR_11_PENALTY=3.140307 PRIMER_LEFT_11_PENALTY=2.023556 PRIMER_RIGHT_11_PENALTY=1.116751 PRIMER_INTERNAL_11_PENALTY=1.054785 PRIMER_LEFT_11_SEQUENCE=ttgttccctccacattctcttt PRIMER_RIGHT_11_SEQUENCE=tgaaggaaagcaagaagcaaa PRIMER_INTERNAL_11_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_11=303,22 PRIMER_RIGHT_11=522,21 PRIMER_INTERNAL_11=332,21 PRIMER_LEFT_11_TM=59.976 PRIMER_RIGHT_11_TM=60.117 PRIMER_INTERNAL_11_TM=59.945 PRIMER_LEFT_11_GC_PERCENT=40.909 PRIMER_RIGHT_11_GC_PERCENT=38.095 PRIMER_INTERNAL_11_GC_PERCENT=38.095 PRIMER_LEFT_11_SELF_ANY=3.00 PRIMER_RIGHT_11_SELF_ANY=2.00 PRIMER_INTERNAL_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_INTERNAL_11_SELF_END=4.00 PRIMER_LEFT_11_END_STABILITY=7.0000 PRIMER_RIGHT_11_END_STABILITY=8.8000 PRIMER_PAIR_11_COMPL_ANY=6.00 PRIMER_PAIR_11_COMPL_END=3.00 PRIMER_PAIR_11_PRODUCT_SIZE=220 PRIMER_PAIR_12_PENALTY=3.252729 PRIMER_LEFT_12_PENALTY=2.023556 PRIMER_RIGHT_12_PENALTY=1.229173 PRIMER_INTERNAL_12_PENALTY=1.054785 PRIMER_LEFT_12_SEQUENCE=tttgttccctccacattctctt PRIMER_RIGHT_12_SEQUENCE=tgaaggaaagcaagaagcaa PRIMER_INTERNAL_12_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_12=302,22 PRIMER_RIGHT_12=522,20 PRIMER_INTERNAL_12=332,21 PRIMER_LEFT_12_TM=59.976 PRIMER_RIGHT_12_TM=58.771 PRIMER_INTERNAL_12_TM=59.945 PRIMER_LEFT_12_GC_PERCENT=40.909 PRIMER_RIGHT_12_GC_PERCENT=40.000 PRIMER_INTERNAL_12_GC_PERCENT=38.095 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=2.00 PRIMER_INTERNAL_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_INTERNAL_12_SELF_END=4.00 PRIMER_LEFT_12_END_STABILITY=6.7000 PRIMER_RIGHT_12_END_STABILITY=8.5000 PRIMER_PAIR_12_COMPL_ANY=6.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PAIR_12_PRODUCT_SIZE=221 PRIMER_PAIR_13_PENALTY=3.299314 PRIMER_LEFT_13_PENALTY=1.188029 PRIMER_RIGHT_13_PENALTY=2.111285 PRIMER_INTERNAL_13_PENALTY=3.306406 PRIMER_LEFT_13_SEQUENCE=atcaacgttccgttcccat PRIMER_RIGHT_13_SEQUENCE=caacagttgtcaaacttgcgt PRIMER_INTERNAL_13_SEQUENCE=ttggaggtgaatttctaggaca PRIMER_LEFT_13=818,19 PRIMER_RIGHT_13=947,21 PRIMER_INTERNAL_13=846,22 PRIMER_LEFT_13_TM=60.188 PRIMER_RIGHT_13_TM=58.889 PRIMER_INTERNAL_13_TM=58.694 PRIMER_LEFT_13_GC_PERCENT=47.368 PRIMER_RIGHT_13_GC_PERCENT=42.857 PRIMER_INTERNAL_13_GC_PERCENT=40.909 PRIMER_LEFT_13_SELF_ANY=6.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_INTERNAL_13_SELF_ANY=5.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_INTERNAL_13_SELF_END=2.00 PRIMER_LEFT_13_END_STABILITY=9.6000 PRIMER_RIGHT_13_END_STABILITY=9.9000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=0.00 PRIMER_PAIR_13_PRODUCT_SIZE=130 PRIMER_PAIR_14_PENALTY=3.404939 PRIMER_LEFT_14_PENALTY=2.237199 PRIMER_RIGHT_14_PENALTY=1.167740 PRIMER_INTERNAL_14_PENALTY=4.688959 PRIMER_LEFT_14_SEQUENCE=ccaaatgtgtaaaagggtgtga PRIMER_RIGHT_14_SEQUENCE=cattcacatgggaacggaac PRIMER_INTERNAL_14_SEQUENCE=tgcaatcaaagattgttcttacc PRIMER_LEFT_14=636,22 PRIMER_RIGHT_14=843,20 PRIMER_INTERNAL_14=720,23 PRIMER_LEFT_14_TM=59.763 PRIMER_RIGHT_14_TM=61.168 PRIMER_INTERNAL_14_TM=58.311 PRIMER_LEFT_14_GC_PERCENT=40.909 PRIMER_RIGHT_14_GC_PERCENT=50.000 PRIMER_INTERNAL_14_GC_PERCENT=34.783 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=8.00 PRIMER_INTERNAL_14_SELF_ANY=7.00 PRIMER_LEFT_14_SELF_END=1.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_INTERNAL_14_SELF_END=0.00 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=7.9000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=0.00 PRIMER_PAIR_14_PRODUCT_SIZE=208 PRIMER_PAIR_15_PENALTY=3.404939 PRIMER_LEFT_15_PENALTY=2.237199 PRIMER_RIGHT_15_PENALTY=1.167740 PRIMER_INTERNAL_15_PENALTY=4.688959 PRIMER_LEFT_15_SEQUENCE=tccaaatgtgtaaaagggtgtg PRIMER_RIGHT_15_SEQUENCE=acattcacatgggaacggaa PRIMER_INTERNAL_15_SEQUENCE=tgcaatcaaagattgttcttacc PRIMER_LEFT_15=635,22 PRIMER_RIGHT_15=844,20 PRIMER_INTERNAL_15=720,23 PRIMER_LEFT_15_TM=59.763 PRIMER_RIGHT_15_TM=61.168 PRIMER_INTERNAL_15_TM=58.311 PRIMER_LEFT_15_GC_PERCENT=40.909 PRIMER_RIGHT_15_GC_PERCENT=45.000 PRIMER_INTERNAL_15_GC_PERCENT=34.783 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=8.00 PRIMER_INTERNAL_15_SELF_ANY=7.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_INTERNAL_15_SELF_END=0.00 PRIMER_LEFT_15_END_STABILITY=6.4000 PRIMER_RIGHT_15_END_STABILITY=10.2000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=210 PRIMER_PAIR_16_PENALTY=3.695091 PRIMER_LEFT_16_PENALTY=2.278124 PRIMER_RIGHT_16_PENALTY=1.416967 PRIMER_INTERNAL_16_PENALTY=3.627789 PRIMER_LEFT_16_SEQUENCE=caatcatgcatgtctgttctga PRIMER_RIGHT_16_SEQUENCE=gatgaaggaaagcaagaagca PRIMER_INTERNAL_16_SEQUENCE=tcataacagcaaaagcatgtgt PRIMER_LEFT_16=343,22 PRIMER_RIGHT_16=524,21 PRIMER_INTERNAL_16=365,22 PRIMER_LEFT_16_TM=59.722 PRIMER_RIGHT_16_TM=59.583 PRIMER_INTERNAL_16_TM=58.372 PRIMER_LEFT_16_GC_PERCENT=40.909 PRIMER_RIGHT_16_GC_PERCENT=42.857 PRIMER_INTERNAL_16_GC_PERCENT=36.364 PRIMER_LEFT_16_SELF_ANY=8.00 PRIMER_RIGHT_16_SELF_ANY=2.00 PRIMER_INTERNAL_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_INTERNAL_16_SELF_END=2.00 PRIMER_LEFT_16_END_STABILITY=6.7000 PRIMER_RIGHT_16_END_STABILITY=8.5000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PAIR_16_PRODUCT_SIZE=182 PRIMER_PAIR_17_PENALTY=3.707313 PRIMER_LEFT_17_PENALTY=2.290346 PRIMER_RIGHT_17_PENALTY=1.416967 PRIMER_INTERNAL_17_PENALTY=1.054785 PRIMER_LEFT_17_SEQUENCE=ttccctccacattctctttg PRIMER_RIGHT_17_SEQUENCE=tgatgaaggaaagcaagaagc PRIMER_INTERNAL_17_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_17=306,20 PRIMER_RIGHT_17=525,21 PRIMER_INTERNAL_17=332,21 PRIMER_LEFT_17_TM=57.710 PRIMER_RIGHT_17_TM=59.583 PRIMER_INTERNAL_17_TM=59.945 PRIMER_LEFT_17_GC_PERCENT=45.000 PRIMER_RIGHT_17_GC_PERCENT=42.857 PRIMER_INTERNAL_17_GC_PERCENT=38.095 PRIMER_LEFT_17_SELF_ANY=2.00 PRIMER_RIGHT_17_SELF_ANY=2.00 PRIMER_INTERNAL_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_INTERNAL_17_SELF_END=4.00 PRIMER_LEFT_17_END_STABILITY=7.3000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PAIR_17_PRODUCT_SIZE=220 PRIMER_PAIR_18_PENALTY=3.755432 PRIMER_LEFT_18_PENALTY=0.727304 PRIMER_RIGHT_18_PENALTY=3.028128 PRIMER_INTERNAL_18_PENALTY=4.087031 PRIMER_LEFT_18_SEQUENCE=gctttccttcatcagccatt PRIMER_RIGHT_18_SEQUENCE=cacacccttttacacatttggat PRIMER_INTERNAL_18_SEQUENCE=tcattctgtttctggattgtgttt PRIMER_LEFT_18=512,20 PRIMER_RIGHT_18=656,23 PRIMER_INTERNAL_18=597,24 PRIMER_LEFT_18_TM=59.273 PRIMER_RIGHT_18_TM=60.028 PRIMER_INTERNAL_18_TM=59.913 PRIMER_LEFT_18_GC_PERCENT=45.000 PRIMER_RIGHT_18_GC_PERCENT=39.130 PRIMER_INTERNAL_18_GC_PERCENT=33.333 PRIMER_LEFT_18_SELF_ANY=3.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_INTERNAL_18_SELF_ANY=3.00 PRIMER_LEFT_18_SELF_END=1.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_INTERNAL_18_SELF_END=0.00 PRIMER_LEFT_18_END_STABILITY=8.4000 PRIMER_RIGHT_18_END_STABILITY=8.1000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PAIR_18_PRODUCT_SIZE=145 PRIMER_PAIR_19_PENALTY=3.784911 PRIMER_LEFT_19_PENALTY=2.367944 PRIMER_RIGHT_19_PENALTY=1.416967 PRIMER_INTERNAL_19_PENALTY=1.054785 PRIMER_LEFT_19_SEQUENCE=tttgttccctccacattctct PRIMER_RIGHT_19_SEQUENCE=gctgatgaaggaaagcaagaa PRIMER_INTERNAL_19_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_19=302,21 PRIMER_RIGHT_19=527,21 PRIMER_INTERNAL_19=332,21 PRIMER_LEFT_19_TM=58.632 PRIMER_RIGHT_19_TM=59.583 PRIMER_INTERNAL_19_TM=59.945 PRIMER_LEFT_19_GC_PERCENT=42.857 PRIMER_RIGHT_19_GC_PERCENT=42.857 PRIMER_INTERNAL_19_GC_PERCENT=38.095 PRIMER_LEFT_19_SELF_ANY=3.00 PRIMER_RIGHT_19_SELF_ANY=3.00 PRIMER_INTERNAL_19_SELF_ANY=4.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_INTERNAL_19_SELF_END=4.00 PRIMER_LEFT_19_END_STABILITY=6.4000 PRIMER_RIGHT_19_END_STABILITY=7.0000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PAIR_19_PRODUCT_SIZE=226 PRIMER_PAIR_20_PENALTY=3.830133 PRIMER_LEFT_20_PENALTY=1.806577 PRIMER_RIGHT_20_PENALTY=2.023556 PRIMER_INTERNAL_20_PENALTY=5.848837 PRIMER_LEFT_20_SEQUENCE=cgattctccccggtatcaa PRIMER_RIGHT_20_SEQUENCE=aaagagaatgtggagggaacaa PRIMER_INTERNAL_20_SEQUENCE=tgtcaactcttattctagccaaca PRIMER_LEFT_20=64,19 PRIMER_RIGHT_20=324,22 PRIMER_INTERNAL_20=273,24 PRIMER_LEFT_20_TM=60.807 PRIMER_RIGHT_20_TM=59.976 PRIMER_INTERNAL_20_TM=58.151 PRIMER_LEFT_20_GC_PERCENT=52.632 PRIMER_RIGHT_20_GC_PERCENT=40.909 PRIMER_INTERNAL_20_GC_PERCENT=37.500 PRIMER_LEFT_20_SELF_ANY=4.00 PRIMER_RIGHT_20_SELF_ANY=3.00 PRIMER_INTERNAL_20_SELF_ANY=4.00 PRIMER_LEFT_20_SELF_END=1.00 PRIMER_RIGHT_20_SELF_END=0.00 PRIMER_INTERNAL_20_SELF_END=1.00 PRIMER_LEFT_20_END_STABILITY=6.9000 PRIMER_RIGHT_20_END_STABILITY=7.0000 PRIMER_PAIR_20_COMPL_ANY=6.00 PRIMER_PAIR_20_COMPL_END=1.00 PRIMER_PAIR_20_PRODUCT_SIZE=261 PRIMER_PAIR_21_PENALTY=4.087459 PRIMER_LEFT_21_PENALTY=1.806597 PRIMER_RIGHT_21_PENALTY=2.280862 PRIMER_INTERNAL_21_PENALTY=1.344173 PRIMER_LEFT_21_SEQUENCE=agattcgattctccccggt PRIMER_RIGHT_21_SEQUENCE=gacatgcatgattggatacgtt PRIMER_INTERNAL_21_SEQUENCE=tccctccacattctctttgaa PRIMER_LEFT_21=59,19 PRIMER_RIGHT_21=356,22 PRIMER_INTERNAL_21=307,21 PRIMER_LEFT_21_TM=60.807 PRIMER_RIGHT_21_TM=59.719 PRIMER_INTERNAL_21_TM=59.656 PRIMER_LEFT_21_GC_PERCENT=52.632 PRIMER_RIGHT_21_GC_PERCENT=40.909 PRIMER_INTERNAL_21_GC_PERCENT=42.857 PRIMER_LEFT_21_SELF_ANY=8.00 PRIMER_RIGHT_21_SELF_ANY=8.00 PRIMER_INTERNAL_21_SELF_ANY=3.00 PRIMER_LEFT_21_SELF_END=2.00 PRIMER_RIGHT_21_SELF_END=2.00 PRIMER_INTERNAL_21_SELF_END=3.00 PRIMER_LEFT_21_END_STABILITY=11.1000 PRIMER_RIGHT_21_END_STABILITY=8.1000 PRIMER_PAIR_21_COMPL_ANY=3.00 PRIMER_PAIR_21_COMPL_END=0.00 PRIMER_PAIR_21_PRODUCT_SIZE=298 PRIMER_PAIR_22_PENALTY=4.106176 PRIMER_LEFT_22_PENALTY=0.744257 PRIMER_RIGHT_22_PENALTY=3.361920 PRIMER_INTERNAL_22_PENALTY=4.087031 PRIMER_LEFT_22_SEQUENCE=tgctttccttcatcagccat PRIMER_RIGHT_22_SEQUENCE=cacccttttacacatttggattc PRIMER_INTERNAL_22_SEQUENCE=tcattctgtttctggattgtgttt PRIMER_LEFT_22=511,20 PRIMER_RIGHT_22=654,23 PRIMER_INTERNAL_22=597,24 PRIMER_LEFT_22_TM=60.744 PRIMER_RIGHT_22_TM=59.638 PRIMER_INTERNAL_22_TM=59.913 PRIMER_LEFT_22_GC_PERCENT=45.000 PRIMER_RIGHT_22_GC_PERCENT=39.130 PRIMER_INTERNAL_22_GC_PERCENT=33.333 PRIMER_LEFT_22_SELF_ANY=3.00 PRIMER_RIGHT_22_SELF_ANY=4.00 PRIMER_INTERNAL_22_SELF_ANY=3.00 PRIMER_LEFT_22_SELF_END=2.00 PRIMER_RIGHT_22_SELF_END=3.00 PRIMER_INTERNAL_22_SELF_END=0.00 PRIMER_LEFT_22_END_STABILITY=9.6000 PRIMER_RIGHT_22_END_STABILITY=6.6000 PRIMER_PAIR_22_COMPL_ANY=3.00 PRIMER_PAIR_22_COMPL_END=0.00 PRIMER_PAIR_22_PRODUCT_SIZE=144 PRIMER_PAIR_23_PENALTY=4.194881 PRIMER_LEFT_23_PENALTY=2.367944 PRIMER_RIGHT_23_PENALTY=1.826937 PRIMER_INTERNAL_23_PENALTY=1.054785 PRIMER_LEFT_23_SEQUENCE=ctttgttccctccacattctc PRIMER_RIGHT_23_SEQUENCE=gctgatgaaggaaagcaaga PRIMER_INTERNAL_23_SEQUENCE=tcaaacgtatccaatcatgca PRIMER_LEFT_23=301,21 PRIMER_RIGHT_23=527,20 PRIMER_INTERNAL_23=332,21 PRIMER_LEFT_23_TM=58.632 PRIMER_RIGHT_23_TM=58.173 PRIMER_INTERNAL_23_TM=59.945 PRIMER_LEFT_23_GC_PERCENT=47.619 PRIMER_RIGHT_23_GC_PERCENT=45.000 PRIMER_INTERNAL_23_GC_PERCENT=38.095 PRIMER_LEFT_23_SELF_ANY=3.00 PRIMER_RIGHT_23_SELF_ANY=3.00 PRIMER_INTERNAL_23_SELF_ANY=4.00 PRIMER_LEFT_23_SELF_END=0.00 PRIMER_RIGHT_23_SELF_END=0.00 PRIMER_INTERNAL_23_SELF_END=4.00 PRIMER_LEFT_23_END_STABILITY=6.7000 PRIMER_RIGHT_23_END_STABILITY=7.0000 PRIMER_PAIR_23_COMPL_ANY=6.00 PRIMER_PAIR_23_COMPL_END=3.00 PRIMER_PAIR_23_PRODUCT_SIZE=227 PRIMER_PAIR_24_PENALTY=4.216158 PRIMER_LEFT_24_PENALTY=3.028128 PRIMER_RIGHT_24_PENALTY=1.188029 PRIMER_INTERNAL_24_PENALTY=4.688959 PRIMER_LEFT_24_SEQUENCE=ccaaatgtgtaaaagggtgtgat PRIMER_RIGHT_24_SEQUENCE=atgggaacggaacgttgat PRIMER_INTERNAL_24_SEQUENCE=tgcaatcaaagattgttcttacc PRIMER_LEFT_24=636,23 PRIMER_RIGHT_24=836,19 PRIMER_INTERNAL_24=720,23 PRIMER_LEFT_24_TM=60.028 PRIMER_RIGHT_24_TM=60.188 PRIMER_INTERNAL_24_TM=58.311 PRIMER_LEFT_24_GC_PERCENT=39.130 PRIMER_RIGHT_24_GC_PERCENT=47.368 PRIMER_INTERNAL_24_GC_PERCENT=34.783 PRIMER_LEFT_24_SELF_ANY=4.00 PRIMER_RIGHT_24_SELF_ANY=6.00 PRIMER_INTERNAL_24_SELF_ANY=7.00 PRIMER_LEFT_24_SELF_END=2.00 PRIMER_RIGHT_24_SELF_END=2.00 PRIMER_INTERNAL_24_SELF_END=0.00 PRIMER_LEFT_24_END_STABILITY=6.3000 PRIMER_RIGHT_24_END_STABILITY=6.9000 PRIMER_PAIR_24_COMPL_ANY=3.00 PRIMER_PAIR_24_COMPL_END=2.00 PRIMER_PAIR_24_PRODUCT_SIZE=201 = SEQUENCE_ID=Pick probes without G at 5 nucleotides the 3 and 5 prime end SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=25 PRIMER_TASK=generic PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 PRIMER_MUST_MATCH_THREE_PRIME=nnnnn PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=hhhhh PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=hhhhh PRIMER_LEFT_EXPLAIN=considered 8496, GC content failed 704, low tm 5558, high tm 529, high end compl 10, long poly-x seq 92, ok 1603 PRIMER_RIGHT_EXPLAIN=considered 8494, GC content failed 723, low tm 5642, high tm 406, high end compl 10, long poly-x seq 92, ok 1621 PRIMER_INTERNAL_EXPLAIN=considered 9631, GC content failed 422, low tm 1156, high tm 51, long poly-x seq 37, failed must_match requirements 7722, ok 243 PRIMER_PAIR_EXPLAIN=considered 2657, unacceptable product size 2620, high end compl 1, primer in pair overlaps a primer in a better pair 2423, ok 36 PRIMER_LEFT_NUM_RETURNED=25 PRIMER_RIGHT_NUM_RETURNED=25 PRIMER_INTERNAL_NUM_RETURNED=25 PRIMER_PAIR_NUM_RETURNED=25 PRIMER_PAIR_0_PENALTY=1.171882 PRIMER_LEFT_0_PENALTY=0.117097 PRIMER_RIGHT_0_PENALTY=1.054785 PRIMER_INTERNAL_0_PENALTY=2.515892 PRIMER_LEFT_0_SEQUENCE=attcgattctccccggtatc PRIMER_RIGHT_0_SEQUENCE=tgcatgattggatacgtttga PRIMER_INTERNAL_0_SEQUENCE=actttgttccctccacattctc PRIMER_LEFT_0=61,20 PRIMER_RIGHT_0=352,21 PRIMER_INTERNAL_0=300,22 PRIMER_LEFT_0_TM=60.117 PRIMER_RIGHT_0_TM=59.945 PRIMER_INTERNAL_0_TM=59.484 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_INTERNAL_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=5.3000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=292 PRIMER_PAIR_1_PENALTY=1.389004 PRIMER_LEFT_1_PENALTY=1.344173 PRIMER_RIGHT_1_PENALTY=0.044832 PRIMER_INTERNAL_1_PENALTY=4.782051 PRIMER_LEFT_1_SEQUENCE=tccctccacattctctttgaa PRIMER_RIGHT_1_SEQUENCE=ggctgatgaaggaaagcaag PRIMER_INTERNAL_1_SEQUENCE=atcatgcatgtctgttctgatcat PRIMER_LEFT_1=307,21 PRIMER_RIGHT_1=528,20 PRIMER_INTERNAL_1=345,24 PRIMER_LEFT_1_TM=59.656 PRIMER_RIGHT_1_TM=59.955 PRIMER_INTERNAL_1_TM=60.782 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=222 PRIMER_PAIR_2_PENALTY=1.461269 PRIMER_LEFT_2_PENALTY=0.117097 PRIMER_RIGHT_2_PENALTY=1.344173 PRIMER_INTERNAL_2_PENALTY=7.331111 PRIMER_LEFT_2_SEQUENCE=gattcgattctccccggtat PRIMER_RIGHT_2_SEQUENCE=tcaaagagaatgtggagggaa PRIMER_INTERNAL_2_SEQUENCE=aacatgtcaactcttattctagccaac PRIMER_LEFT_2=60,20 PRIMER_RIGHT_2=326,21 PRIMER_INTERNAL_2=269,27 PRIMER_LEFT_2_TM=60.117 PRIMER_RIGHT_2_TM=59.656 PRIMER_INTERNAL_2_TM=60.331 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_INTERNAL_2_GC_PERCENT=37.037 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.8000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=267 PRIMER_PAIR_3_PENALTY=2.071477 PRIMER_LEFT_3_PENALTY=1.344173 PRIMER_RIGHT_3_PENALTY=0.727304 PRIMER_INTERNAL_3_PENALTY=4.782051 PRIMER_LEFT_3_SEQUENCE=ttccctccacattctctttga PRIMER_RIGHT_3_SEQUENCE=aatggctgatgaaggaaagc PRIMER_INTERNAL_3_SEQUENCE=atcatgcatgtctgttctgatcat PRIMER_LEFT_3=306,21 PRIMER_RIGHT_3=531,20 PRIMER_INTERNAL_3=345,24 PRIMER_LEFT_3_TM=59.656 PRIMER_RIGHT_3_TM=59.273 PRIMER_INTERNAL_3_TM=60.782 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=37.500 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=226 PRIMER_PAIR_4_PENALTY=2.122810 PRIMER_LEFT_4_PENALTY=1.058858 PRIMER_RIGHT_4_PENALTY=1.063952 PRIMER_INTERNAL_4_PENALTY=6.476933 PRIMER_LEFT_4_SEQUENCE=cccatgtgaatgtattggagg PRIMER_RIGHT_4_SEQUENCE=tcaacagttgtcaaacttgcg PRIMER_INTERNAL_4_SEQUENCE=acaacaaaagaaggagacgattat PRIMER_LEFT_4=832,21 PRIMER_RIGHT_4=948,21 PRIMER_INTERNAL_4=881,24 PRIMER_LEFT_4_TM=60.059 PRIMER_RIGHT_4_TM=59.936 PRIMER_INTERNAL_4_TM=57.523 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_INTERNAL_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=10.5000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=117 PRIMER_PAIR_5_PENALTY=2.131001 PRIMER_LEFT_5_PENALTY=0.786828 PRIMER_RIGHT_5_PENALTY=1.344173 PRIMER_INTERNAL_5_PENALTY=7.331111 PRIMER_LEFT_5_SEQUENCE=agattcgattctccccggta PRIMER_RIGHT_5_SEQUENCE=ttcaaagagaatgtggaggga PRIMER_INTERNAL_5_SEQUENCE=aacatgtcaactcttattctagccaac PRIMER_LEFT_5=59,20 PRIMER_RIGHT_5=327,21 PRIMER_INTERNAL_5=269,27 PRIMER_LEFT_5_TM=60.787 PRIMER_RIGHT_5_TM=59.656 PRIMER_INTERNAL_5_TM=60.331 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=42.857 PRIMER_INTERNAL_5_GC_PERCENT=37.037 PRIMER_LEFT_5_SELF_ANY=8.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_INTERNAL_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_INTERNAL_5_SELF_END=0.00 PRIMER_LEFT_5_END_STABILITY=8.9000 PRIMER_RIGHT_5_END_STABILITY=9.4000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=269 PRIMER_PAIR_6_PENALTY=2.227098 PRIMER_LEFT_6_PENALTY=1.482841 PRIMER_RIGHT_6_PENALTY=0.744257 PRIMER_INTERNAL_6_PENALTY=5.033056 PRIMER_LEFT_6_SEQUENCE=tccaatcatgcatgtctgttc PRIMER_RIGHT_6_SEQUENCE=atggctgatgaaggaaagca PRIMER_INTERNAL_6_SEQUENCE=tttttgctgatttgagattctttc PRIMER_LEFT_6=341,21 PRIMER_RIGHT_6=530,20 PRIMER_INTERNAL_6=474,24 PRIMER_LEFT_6_TM=59.517 PRIMER_RIGHT_6_TM=60.744 PRIMER_INTERNAL_6_TM=58.967 PRIMER_LEFT_6_GC_PERCENT=42.857 PRIMER_RIGHT_6_GC_PERCENT=45.000 PRIMER_INTERNAL_6_GC_PERCENT=29.167 PRIMER_LEFT_6_SELF_ANY=8.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_INTERNAL_6_SELF_ANY=5.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_INTERNAL_6_SELF_END=1.00 PRIMER_LEFT_6_END_STABILITY=6.7000 PRIMER_RIGHT_6_END_STABILITY=8.5000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=190 PRIMER_PAIR_7_PENALTY=2.282031 PRIMER_LEFT_7_PENALTY=0.044832 PRIMER_RIGHT_7_PENALTY=2.237199 PRIMER_INTERNAL_7_PENALTY=5.614815 PRIMER_LEFT_7_SEQUENCE=cttgctttccttcatcagcc PRIMER_RIGHT_7_SEQUENCE=tcacacccttttacacatttgg PRIMER_INTERNAL_7_SEQUENCE=tctcttcttgattcaatgaatctca PRIMER_LEFT_7=509,20 PRIMER_RIGHT_7=657,22 PRIMER_INTERNAL_7=554,25 PRIMER_LEFT_7_TM=59.955 PRIMER_RIGHT_7_TM=59.763 PRIMER_INTERNAL_7_TM=59.385 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=40.909 PRIMER_INTERNAL_7_GC_PERCENT=32.000 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_INTERNAL_7_SELF_ANY=11.00 PRIMER_LEFT_7_SELF_END=1.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_INTERNAL_7_SELF_END=7.00 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=8.8000 PRIMER_PAIR_7_COMPL_ANY=2.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PAIR_7_PRODUCT_SIZE=149 PRIMER_PAIR_8_PENALTY=2.676544 PRIMER_LEFT_8_PENALTY=0.833045 PRIMER_RIGHT_8_PENALTY=1.843499 PRIMER_INTERNAL_8_PENALTY=7.331111 PRIMER_LEFT_8_SEQUENCE=tctcagattcgattctcccc PRIMER_RIGHT_8_SEQUENCE=tttcaaagagaatgtggaggg PRIMER_INTERNAL_8_SEQUENCE=aacatgtcaactcttattctagccaac PRIMER_LEFT_8=55,20 PRIMER_RIGHT_8=328,21 PRIMER_INTERNAL_8=269,27 PRIMER_LEFT_8_TM=59.167 PRIMER_RIGHT_8_TM=59.157 PRIMER_INTERNAL_8_TM=60.331 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=42.857 PRIMER_INTERNAL_8_GC_PERCENT=37.037 PRIMER_LEFT_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_INTERNAL_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_INTERNAL_8_SELF_END=0.00 PRIMER_LEFT_8_END_STABILITY=10.9000 PRIMER_RIGHT_8_END_STABILITY=9.4000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PAIR_8_PRODUCT_SIZE=274 PRIMER_PAIR_9_PENALTY=3.041124 PRIMER_LEFT_9_PENALTY=1.029407 PRIMER_RIGHT_9_PENALTY=2.011716 PRIMER_INTERNAL_9_PENALTY=2.515892 PRIMER_LEFT_9_SEQUENCE=ggtctcagattcgattctccc PRIMER_RIGHT_9_SEQUENCE=tgcatgattggatacgtttg PRIMER_INTERNAL_9_SEQUENCE=actttgttccctccacattctc PRIMER_LEFT_9=53,21 PRIMER_RIGHT_9=352,20 PRIMER_INTERNAL_9=300,22 PRIMER_LEFT_9_TM=60.029 PRIMER_RIGHT_9_TM=57.988 PRIMER_INTERNAL_9_TM=59.484 PRIMER_LEFT_9_GC_PERCENT=52.381 PRIMER_RIGHT_9_GC_PERCENT=40.000 PRIMER_INTERNAL_9_GC_PERCENT=45.455 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_INTERNAL_9_SELF_ANY=3.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_INTERNAL_9_SELF_END=0.00 PRIMER_LEFT_9_END_STABILITY=9.4000 PRIMER_RIGHT_9_END_STABILITY=7.0000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=300 PRIMER_PAIR_10_PENALTY=3.057112 PRIMER_LEFT_10_PENALTY=1.033556 PRIMER_RIGHT_10_PENALTY=2.023556 PRIMER_INTERNAL_10_PENALTY=7.331111 PRIMER_LEFT_10_SEQUENCE=cgattctccccggtatcaat PRIMER_RIGHT_10_SEQUENCE=aagagaatgtggagggaacaaa PRIMER_INTERNAL_10_SEQUENCE=aacatgtcaactcttattctagccaac PRIMER_LEFT_10=64,20 PRIMER_RIGHT_10=323,22 PRIMER_INTERNAL_10=269,27 PRIMER_LEFT_10_TM=61.034 PRIMER_RIGHT_10_TM=59.976 PRIMER_INTERNAL_10_TM=60.331 PRIMER_LEFT_10_GC_PERCENT=50.000 PRIMER_RIGHT_10_GC_PERCENT=40.909 PRIMER_INTERNAL_10_GC_PERCENT=37.037 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=3.00 PRIMER_INTERNAL_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_INTERNAL_10_SELF_END=0.00 PRIMER_LEFT_10_END_STABILITY=6.9000 PRIMER_RIGHT_10_END_STABILITY=7.0000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=260 PRIMER_PAIR_11_PENALTY=3.140307 PRIMER_LEFT_11_PENALTY=2.023556 PRIMER_RIGHT_11_PENALTY=1.116751 PRIMER_INTERNAL_11_PENALTY=4.782051 PRIMER_LEFT_11_SEQUENCE=ttgttccctccacattctcttt PRIMER_RIGHT_11_SEQUENCE=tgaaggaaagcaagaagcaaa PRIMER_INTERNAL_11_SEQUENCE=atcatgcatgtctgttctgatcat PRIMER_LEFT_11=303,22 PRIMER_RIGHT_11=522,21 PRIMER_INTERNAL_11=345,24 PRIMER_LEFT_11_TM=59.976 PRIMER_RIGHT_11_TM=60.117 PRIMER_INTERNAL_11_TM=60.782 PRIMER_LEFT_11_GC_PERCENT=40.909 PRIMER_RIGHT_11_GC_PERCENT=38.095 PRIMER_INTERNAL_11_GC_PERCENT=37.500 PRIMER_LEFT_11_SELF_ANY=3.00 PRIMER_RIGHT_11_SELF_ANY=2.00 PRIMER_INTERNAL_11_SELF_ANY=8.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_INTERNAL_11_SELF_END=4.00 PRIMER_LEFT_11_END_STABILITY=7.0000 PRIMER_RIGHT_11_END_STABILITY=8.8000 PRIMER_PAIR_11_COMPL_ANY=6.00 PRIMER_PAIR_11_COMPL_END=3.00 PRIMER_PAIR_11_PRODUCT_SIZE=220 PRIMER_PAIR_12_PENALTY=3.252729 PRIMER_LEFT_12_PENALTY=2.023556 PRIMER_RIGHT_12_PENALTY=1.229173 PRIMER_INTERNAL_12_PENALTY=4.782051 PRIMER_LEFT_12_SEQUENCE=tttgttccctccacattctctt PRIMER_RIGHT_12_SEQUENCE=tgaaggaaagcaagaagcaa PRIMER_INTERNAL_12_SEQUENCE=atcatgcatgtctgttctgatcat PRIMER_LEFT_12=302,22 PRIMER_RIGHT_12=522,20 PRIMER_INTERNAL_12=345,24 PRIMER_LEFT_12_TM=59.976 PRIMER_RIGHT_12_TM=58.771 PRIMER_INTERNAL_12_TM=60.782 PRIMER_LEFT_12_GC_PERCENT=40.909 PRIMER_RIGHT_12_GC_PERCENT=40.000 PRIMER_INTERNAL_12_GC_PERCENT=37.500 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=2.00 PRIMER_INTERNAL_12_SELF_ANY=8.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_INTERNAL_12_SELF_END=4.00 PRIMER_LEFT_12_END_STABILITY=6.7000 PRIMER_RIGHT_12_END_STABILITY=8.5000 PRIMER_PAIR_12_COMPL_ANY=6.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PAIR_12_PRODUCT_SIZE=221 PRIMER_PAIR_13_PENALTY=3.299314 PRIMER_LEFT_13_PENALTY=1.188029 PRIMER_RIGHT_13_PENALTY=2.111285 PRIMER_INTERNAL_13_PENALTY=6.476933 PRIMER_LEFT_13_SEQUENCE=atcaacgttccgttcccat PRIMER_RIGHT_13_SEQUENCE=caacagttgtcaaacttgcgt PRIMER_INTERNAL_13_SEQUENCE=acaacaaaagaaggagacgattat PRIMER_LEFT_13=818,19 PRIMER_RIGHT_13=947,21 PRIMER_INTERNAL_13=881,24 PRIMER_LEFT_13_TM=60.188 PRIMER_RIGHT_13_TM=58.889 PRIMER_INTERNAL_13_TM=57.523 PRIMER_LEFT_13_GC_PERCENT=47.368 PRIMER_RIGHT_13_GC_PERCENT=42.857 PRIMER_INTERNAL_13_GC_PERCENT=33.333 PRIMER_LEFT_13_SELF_ANY=6.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_INTERNAL_13_SELF_ANY=3.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_INTERNAL_13_SELF_END=3.00 PRIMER_LEFT_13_END_STABILITY=9.6000 PRIMER_RIGHT_13_END_STABILITY=9.9000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=0.00 PRIMER_PAIR_13_PRODUCT_SIZE=130 PRIMER_PAIR_14_PENALTY=3.404939 PRIMER_LEFT_14_PENALTY=2.237199 PRIMER_RIGHT_14_PENALTY=1.167740 PRIMER_INTERNAL_14_PENALTY=5.389860 PRIMER_LEFT_14_SEQUENCE=ccaaatgtgtaaaagggtgtga PRIMER_RIGHT_14_SEQUENCE=cattcacatgggaacggaac PRIMER_INTERNAL_14_SEQUENCE=tcaaagattgttcttaccaattcc PRIMER_LEFT_14=636,22 PRIMER_RIGHT_14=843,20 PRIMER_INTERNAL_14=725,24 PRIMER_LEFT_14_TM=59.763 PRIMER_RIGHT_14_TM=61.168 PRIMER_INTERNAL_14_TM=58.610 PRIMER_LEFT_14_GC_PERCENT=40.909 PRIMER_RIGHT_14_GC_PERCENT=50.000 PRIMER_INTERNAL_14_GC_PERCENT=33.333 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=8.00 PRIMER_INTERNAL_14_SELF_ANY=5.00 PRIMER_LEFT_14_SELF_END=1.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_INTERNAL_14_SELF_END=1.00 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=7.9000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=0.00 PRIMER_PAIR_14_PRODUCT_SIZE=208 PRIMER_PAIR_15_PENALTY=3.404939 PRIMER_LEFT_15_PENALTY=2.237199 PRIMER_RIGHT_15_PENALTY=1.167740 PRIMER_INTERNAL_15_PENALTY=5.389860 PRIMER_LEFT_15_SEQUENCE=tccaaatgtgtaaaagggtgtg PRIMER_RIGHT_15_SEQUENCE=acattcacatgggaacggaa PRIMER_INTERNAL_15_SEQUENCE=tcaaagattgttcttaccaattcc PRIMER_LEFT_15=635,22 PRIMER_RIGHT_15=844,20 PRIMER_INTERNAL_15=725,24 PRIMER_LEFT_15_TM=59.763 PRIMER_RIGHT_15_TM=61.168 PRIMER_INTERNAL_15_TM=58.610 PRIMER_LEFT_15_GC_PERCENT=40.909 PRIMER_RIGHT_15_GC_PERCENT=45.000 PRIMER_INTERNAL_15_GC_PERCENT=33.333 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=8.00 PRIMER_INTERNAL_15_SELF_ANY=5.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_INTERNAL_15_SELF_END=1.00 PRIMER_LEFT_15_END_STABILITY=6.4000 PRIMER_RIGHT_15_END_STABILITY=10.2000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=210 PRIMER_PAIR_16_PENALTY=3.695091 PRIMER_LEFT_16_PENALTY=2.278124 PRIMER_RIGHT_16_PENALTY=1.416967 PRIMER_INTERNAL_16_PENALTY=5.033056 PRIMER_LEFT_16_SEQUENCE=caatcatgcatgtctgttctga PRIMER_RIGHT_16_SEQUENCE=gatgaaggaaagcaagaagca PRIMER_INTERNAL_16_SEQUENCE=tttttgctgatttgagattctttc PRIMER_LEFT_16=343,22 PRIMER_RIGHT_16=524,21 PRIMER_INTERNAL_16=474,24 PRIMER_LEFT_16_TM=59.722 PRIMER_RIGHT_16_TM=59.583 PRIMER_INTERNAL_16_TM=58.967 PRIMER_LEFT_16_GC_PERCENT=40.909 PRIMER_RIGHT_16_GC_PERCENT=42.857 PRIMER_INTERNAL_16_GC_PERCENT=29.167 PRIMER_LEFT_16_SELF_ANY=8.00 PRIMER_RIGHT_16_SELF_ANY=2.00 PRIMER_INTERNAL_16_SELF_ANY=5.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_INTERNAL_16_SELF_END=1.00 PRIMER_LEFT_16_END_STABILITY=6.7000 PRIMER_RIGHT_16_END_STABILITY=8.5000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PAIR_16_PRODUCT_SIZE=182 PRIMER_PAIR_17_PENALTY=3.707313 PRIMER_LEFT_17_PENALTY=2.290346 PRIMER_RIGHT_17_PENALTY=1.416967 PRIMER_INTERNAL_17_PENALTY=4.782051 PRIMER_LEFT_17_SEQUENCE=ttccctccacattctctttg PRIMER_RIGHT_17_SEQUENCE=tgatgaaggaaagcaagaagc PRIMER_INTERNAL_17_SEQUENCE=atcatgcatgtctgttctgatcat PRIMER_LEFT_17=306,20 PRIMER_RIGHT_17=525,21 PRIMER_INTERNAL_17=345,24 PRIMER_LEFT_17_TM=57.710 PRIMER_RIGHT_17_TM=59.583 PRIMER_INTERNAL_17_TM=60.782 PRIMER_LEFT_17_GC_PERCENT=45.000 PRIMER_RIGHT_17_GC_PERCENT=42.857 PRIMER_INTERNAL_17_GC_PERCENT=37.500 PRIMER_LEFT_17_SELF_ANY=2.00 PRIMER_RIGHT_17_SELF_ANY=2.00 PRIMER_INTERNAL_17_SELF_ANY=8.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_INTERNAL_17_SELF_END=4.00 PRIMER_LEFT_17_END_STABILITY=7.3000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PAIR_17_PRODUCT_SIZE=220 PRIMER_PAIR_18_PENALTY=3.755432 PRIMER_LEFT_18_PENALTY=0.727304 PRIMER_RIGHT_18_PENALTY=3.028128 PRIMER_INTERNAL_18_PENALTY=5.614815 PRIMER_LEFT_18_SEQUENCE=gctttccttcatcagccatt PRIMER_RIGHT_18_SEQUENCE=cacacccttttacacatttggat PRIMER_INTERNAL_18_SEQUENCE=tctcttcttgattcaatgaatctca PRIMER_LEFT_18=512,20 PRIMER_RIGHT_18=656,23 PRIMER_INTERNAL_18=554,25 PRIMER_LEFT_18_TM=59.273 PRIMER_RIGHT_18_TM=60.028 PRIMER_INTERNAL_18_TM=59.385 PRIMER_LEFT_18_GC_PERCENT=45.000 PRIMER_RIGHT_18_GC_PERCENT=39.130 PRIMER_INTERNAL_18_GC_PERCENT=32.000 PRIMER_LEFT_18_SELF_ANY=3.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_INTERNAL_18_SELF_ANY=11.00 PRIMER_LEFT_18_SELF_END=1.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_INTERNAL_18_SELF_END=7.00 PRIMER_LEFT_18_END_STABILITY=8.4000 PRIMER_RIGHT_18_END_STABILITY=8.1000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PAIR_18_PRODUCT_SIZE=145 PRIMER_PAIR_19_PENALTY=3.784911 PRIMER_LEFT_19_PENALTY=2.367944 PRIMER_RIGHT_19_PENALTY=1.416967 PRIMER_INTERNAL_19_PENALTY=4.782051 PRIMER_LEFT_19_SEQUENCE=tttgttccctccacattctct PRIMER_RIGHT_19_SEQUENCE=gctgatgaaggaaagcaagaa PRIMER_INTERNAL_19_SEQUENCE=atcatgcatgtctgttctgatcat PRIMER_LEFT_19=302,21 PRIMER_RIGHT_19=527,21 PRIMER_INTERNAL_19=345,24 PRIMER_LEFT_19_TM=58.632 PRIMER_RIGHT_19_TM=59.583 PRIMER_INTERNAL_19_TM=60.782 PRIMER_LEFT_19_GC_PERCENT=42.857 PRIMER_RIGHT_19_GC_PERCENT=42.857 PRIMER_INTERNAL_19_GC_PERCENT=37.500 PRIMER_LEFT_19_SELF_ANY=3.00 PRIMER_RIGHT_19_SELF_ANY=3.00 PRIMER_INTERNAL_19_SELF_ANY=8.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_INTERNAL_19_SELF_END=4.00 PRIMER_LEFT_19_END_STABILITY=6.4000 PRIMER_RIGHT_19_END_STABILITY=7.0000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PAIR_19_PRODUCT_SIZE=226 PRIMER_PAIR_20_PENALTY=3.830133 PRIMER_LEFT_20_PENALTY=1.806577 PRIMER_RIGHT_20_PENALTY=2.023556 PRIMER_INTERNAL_20_PENALTY=7.331111 PRIMER_LEFT_20_SEQUENCE=cgattctccccggtatcaa PRIMER_RIGHT_20_SEQUENCE=aaagagaatgtggagggaacaa PRIMER_INTERNAL_20_SEQUENCE=aacatgtcaactcttattctagccaac PRIMER_LEFT_20=64,19 PRIMER_RIGHT_20=324,22 PRIMER_INTERNAL_20=269,27 PRIMER_LEFT_20_TM=60.807 PRIMER_RIGHT_20_TM=59.976 PRIMER_INTERNAL_20_TM=60.331 PRIMER_LEFT_20_GC_PERCENT=52.632 PRIMER_RIGHT_20_GC_PERCENT=40.909 PRIMER_INTERNAL_20_GC_PERCENT=37.037 PRIMER_LEFT_20_SELF_ANY=4.00 PRIMER_RIGHT_20_SELF_ANY=3.00 PRIMER_INTERNAL_20_SELF_ANY=6.00 PRIMER_LEFT_20_SELF_END=1.00 PRIMER_RIGHT_20_SELF_END=0.00 PRIMER_INTERNAL_20_SELF_END=0.00 PRIMER_LEFT_20_END_STABILITY=6.9000 PRIMER_RIGHT_20_END_STABILITY=7.0000 PRIMER_PAIR_20_COMPL_ANY=6.00 PRIMER_PAIR_20_COMPL_END=1.00 PRIMER_PAIR_20_PRODUCT_SIZE=261 PRIMER_PAIR_21_PENALTY=4.087459 PRIMER_LEFT_21_PENALTY=1.806597 PRIMER_RIGHT_21_PENALTY=2.280862 PRIMER_INTERNAL_21_PENALTY=2.515892 PRIMER_LEFT_21_SEQUENCE=agattcgattctccccggt PRIMER_RIGHT_21_SEQUENCE=gacatgcatgattggatacgtt PRIMER_INTERNAL_21_SEQUENCE=actttgttccctccacattctc PRIMER_LEFT_21=59,19 PRIMER_RIGHT_21=356,22 PRIMER_INTERNAL_21=300,22 PRIMER_LEFT_21_TM=60.807 PRIMER_RIGHT_21_TM=59.719 PRIMER_INTERNAL_21_TM=59.484 PRIMER_LEFT_21_GC_PERCENT=52.632 PRIMER_RIGHT_21_GC_PERCENT=40.909 PRIMER_INTERNAL_21_GC_PERCENT=45.455 PRIMER_LEFT_21_SELF_ANY=8.00 PRIMER_RIGHT_21_SELF_ANY=8.00 PRIMER_INTERNAL_21_SELF_ANY=3.00 PRIMER_LEFT_21_SELF_END=2.00 PRIMER_RIGHT_21_SELF_END=2.00 PRIMER_INTERNAL_21_SELF_END=0.00 PRIMER_LEFT_21_END_STABILITY=11.1000 PRIMER_RIGHT_21_END_STABILITY=8.1000 PRIMER_PAIR_21_COMPL_ANY=3.00 PRIMER_PAIR_21_COMPL_END=0.00 PRIMER_PAIR_21_PRODUCT_SIZE=298 PRIMER_PAIR_22_PENALTY=4.106176 PRIMER_LEFT_22_PENALTY=0.744257 PRIMER_RIGHT_22_PENALTY=3.361920 PRIMER_INTERNAL_22_PENALTY=5.614815 PRIMER_LEFT_22_SEQUENCE=tgctttccttcatcagccat PRIMER_RIGHT_22_SEQUENCE=cacccttttacacatttggattc PRIMER_INTERNAL_22_SEQUENCE=tctcttcttgattcaatgaatctca PRIMER_LEFT_22=511,20 PRIMER_RIGHT_22=654,23 PRIMER_INTERNAL_22=554,25 PRIMER_LEFT_22_TM=60.744 PRIMER_RIGHT_22_TM=59.638 PRIMER_INTERNAL_22_TM=59.385 PRIMER_LEFT_22_GC_PERCENT=45.000 PRIMER_RIGHT_22_GC_PERCENT=39.130 PRIMER_INTERNAL_22_GC_PERCENT=32.000 PRIMER_LEFT_22_SELF_ANY=3.00 PRIMER_RIGHT_22_SELF_ANY=4.00 PRIMER_INTERNAL_22_SELF_ANY=11.00 PRIMER_LEFT_22_SELF_END=2.00 PRIMER_RIGHT_22_SELF_END=3.00 PRIMER_INTERNAL_22_SELF_END=7.00 PRIMER_LEFT_22_END_STABILITY=9.6000 PRIMER_RIGHT_22_END_STABILITY=6.6000 PRIMER_PAIR_22_COMPL_ANY=3.00 PRIMER_PAIR_22_COMPL_END=0.00 PRIMER_PAIR_22_PRODUCT_SIZE=144 PRIMER_PAIR_23_PENALTY=4.194881 PRIMER_LEFT_23_PENALTY=2.367944 PRIMER_RIGHT_23_PENALTY=1.826937 PRIMER_INTERNAL_23_PENALTY=4.782051 PRIMER_LEFT_23_SEQUENCE=ctttgttccctccacattctc PRIMER_RIGHT_23_SEQUENCE=gctgatgaaggaaagcaaga PRIMER_INTERNAL_23_SEQUENCE=atcatgcatgtctgttctgatcat PRIMER_LEFT_23=301,21 PRIMER_RIGHT_23=527,20 PRIMER_INTERNAL_23=345,24 PRIMER_LEFT_23_TM=58.632 PRIMER_RIGHT_23_TM=58.173 PRIMER_INTERNAL_23_TM=60.782 PRIMER_LEFT_23_GC_PERCENT=47.619 PRIMER_RIGHT_23_GC_PERCENT=45.000 PRIMER_INTERNAL_23_GC_PERCENT=37.500 PRIMER_LEFT_23_SELF_ANY=3.00 PRIMER_RIGHT_23_SELF_ANY=3.00 PRIMER_INTERNAL_23_SELF_ANY=8.00 PRIMER_LEFT_23_SELF_END=0.00 PRIMER_RIGHT_23_SELF_END=0.00 PRIMER_INTERNAL_23_SELF_END=4.00 PRIMER_LEFT_23_END_STABILITY=6.7000 PRIMER_RIGHT_23_END_STABILITY=7.0000 PRIMER_PAIR_23_COMPL_ANY=6.00 PRIMER_PAIR_23_COMPL_END=3.00 PRIMER_PAIR_23_PRODUCT_SIZE=227 PRIMER_PAIR_24_PENALTY=4.216158 PRIMER_LEFT_24_PENALTY=3.028128 PRIMER_RIGHT_24_PENALTY=1.188029 PRIMER_INTERNAL_24_PENALTY=5.389860 PRIMER_LEFT_24_SEQUENCE=ccaaatgtgtaaaagggtgtgat PRIMER_RIGHT_24_SEQUENCE=atgggaacggaacgttgat PRIMER_INTERNAL_24_SEQUENCE=tcaaagattgttcttaccaattcc PRIMER_LEFT_24=636,23 PRIMER_RIGHT_24=836,19 PRIMER_INTERNAL_24=725,24 PRIMER_LEFT_24_TM=60.028 PRIMER_RIGHT_24_TM=60.188 PRIMER_INTERNAL_24_TM=58.610 PRIMER_LEFT_24_GC_PERCENT=39.130 PRIMER_RIGHT_24_GC_PERCENT=47.368 PRIMER_INTERNAL_24_GC_PERCENT=33.333 PRIMER_LEFT_24_SELF_ANY=4.00 PRIMER_RIGHT_24_SELF_ANY=6.00 PRIMER_INTERNAL_24_SELF_ANY=5.00 PRIMER_LEFT_24_SELF_END=2.00 PRIMER_RIGHT_24_SELF_END=2.00 PRIMER_INTERNAL_24_SELF_END=1.00 PRIMER_LEFT_24_END_STABILITY=6.3000 PRIMER_RIGHT_24_END_STABILITY=6.9000 PRIMER_PAIR_24_COMPL_ANY=3.00 PRIMER_PAIR_24_COMPL_END=2.00 PRIMER_PAIR_24_PRODUCT_SIZE=201 = SEQUENCE_ID=Primers must end in AG SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=25 PRIMER_TASK=generic PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 PRIMER_MUST_MATCH_THREE_PRIME=nnnAG PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn PRIMER_LEFT_EXPLAIN=considered 9032, GC content failed 12, low tm 301, high tm 19, high any compl 8, high end compl 11, long poly-x seq 2, failed must_match requirements 8638, ok 41 PRIMER_RIGHT_EXPLAIN=considered 9023, GC content failed 27, low tm 420, high tm 32, high any compl 2, high end compl 12, long poly-x seq 5, failed must_match requirements 8440, ok 85 PRIMER_INTERNAL_EXPLAIN=considered 9631, GC content failed 422, low tm 1156, high tm 51, long poly-x seq 37, failed must_match requirements 7722, ok 243 PRIMER_PAIR_EXPLAIN=considered 1708, unacceptable product size 1695, high end compl 3, no internal oligo 2, primer in pair overlaps a primer in a better pair 1352, ok 8 PRIMER_LEFT_NUM_RETURNED=8 PRIMER_RIGHT_NUM_RETURNED=8 PRIMER_INTERNAL_NUM_RETURNED=8 PRIMER_PAIR_NUM_RETURNED=8 PRIMER_PAIR_0_PENALTY=5.015480 PRIMER_LEFT_0_PENALTY=2.647536 PRIMER_RIGHT_0_PENALTY=2.367944 PRIMER_INTERNAL_0_PENALTY=7.331111 PRIMER_LEFT_0_SEQUENCE=ttctccccggtatcaatttaag PRIMER_RIGHT_0_SEQUENCE=gagaatgtggagggaacaaag PRIMER_INTERNAL_0_SEQUENCE=aacatgtcaactcttattctagccaac PRIMER_LEFT_0=67,22 PRIMER_RIGHT_0=321,21 PRIMER_INTERNAL_0=269,27 PRIMER_LEFT_0_TM=59.352 PRIMER_RIGHT_0_TM=58.632 PRIMER_INTERNAL_0_TM=60.331 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_INTERNAL_0_GC_PERCENT=37.037 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.3000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=255 PRIMER_PAIR_1_PENALTY=5.977824 PRIMER_LEFT_1_PENALTY=5.932992 PRIMER_RIGHT_1_PENALTY=0.044832 PRIMER_INTERNAL_1_PENALTY=2.515892 PRIMER_LEFT_1_SEQUENCE=tgtcaactcttattctagccaacag PRIMER_RIGHT_1_SEQUENCE=ggctgatgaaggaaagcaag PRIMER_INTERNAL_1_SEQUENCE=actttgttccctccacattctc PRIMER_LEFT_1=273,25 PRIMER_RIGHT_1=528,20 PRIMER_INTERNAL_1=300,22 PRIMER_LEFT_1_TM=59.067 PRIMER_RIGHT_1_TM=59.955 PRIMER_INTERNAL_1_TM=59.484 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=256 PRIMER_PAIR_2_PENALTY=8.162202 PRIMER_LEFT_2_PENALTY=2.314970 PRIMER_RIGHT_2_PENALTY=5.847232 PRIMER_INTERNAL_2_PENALTY=6.476933 PRIMER_LEFT_2_SEQUENCE=ttcccatgtgaatgtattggag PRIMER_RIGHT_2_SEQUENCE=cagttgtcaaacttgcgtaataag PRIMER_INTERNAL_2_SEQUENCE=acaacaaaagaaggagacgattat PRIMER_LEFT_2=830,22 PRIMER_RIGHT_2=944,24 PRIMER_INTERNAL_2=881,24 PRIMER_LEFT_2_TM=59.685 PRIMER_RIGHT_2_TM=58.153 PRIMER_INTERNAL_2_TM=57.523 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=37.500 PRIMER_INTERNAL_2_GC_PERCENT=33.333 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=5.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=115 PRIMER_PAIR_3_PENALTY=8.662949 PRIMER_LEFT_3_PENALTY=6.329720 PRIMER_RIGHT_3_PENALTY=2.333229 PRIMER_INTERNAL_3_PENALTY=5.707700 PRIMER_LEFT_3_SEQUENCE=tcataacagcaaaagcatgtgtatag PRIMER_RIGHT_3_SEQUENCE=ggaaagcaagaagcaaagaaag PRIMER_INTERNAL_3_SEQUENCE=tttttgctgatttgagattcttt PRIMER_LEFT_3=365,26 PRIMER_RIGHT_3=518,22 PRIMER_INTERNAL_3=474,23 PRIMER_LEFT_3_TM=59.670 PRIMER_RIGHT_3_TM=59.667 PRIMER_INTERNAL_3_TM=57.292 PRIMER_LEFT_3_GC_PERCENT=34.615 PRIMER_RIGHT_3_GC_PERCENT=40.909 PRIMER_INTERNAL_3_GC_PERCENT=26.087 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=4.9000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=2.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=154 PRIMER_PAIR_4_PENALTY=10.238277 PRIMER_LEFT_4_PENALTY=4.180440 PRIMER_RIGHT_4_PENALTY=6.057837 PRIMER_INTERNAL_4_PENALTY=2.564187 PRIMER_LEFT_4_SEQUENCE=tcaacatcttcgtaaaggtctcag PRIMER_RIGHT_4_SEQUENCE=ttgactatttcaaagagaatgtggag PRIMER_INTERNAL_4_SEQUENCE=ttctccccggtatcaatttaa PRIMER_LEFT_4=37,24 PRIMER_RIGHT_4=335,26 PRIMER_INTERNAL_4=67,21 PRIMER_LEFT_4_TM=59.820 PRIMER_RIGHT_4_TM=60.058 PRIMER_INTERNAL_4_TM=58.436 PRIMER_LEFT_4_GC_PERCENT=41.667 PRIMER_RIGHT_4_GC_PERCENT=34.615 PRIMER_INTERNAL_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=299 PRIMER_PAIR_5_PENALTY=10.983089 PRIMER_LEFT_5_PENALTY=2.502561 PRIMER_RIGHT_5_PENALTY=8.480528 PRIMER_INTERNAL_5_PENALTY=5.614815 PRIMER_LEFT_5_SEQUENCE=gcttcttgctttccttcatcag PRIMER_RIGHT_5_SEQUENCE=cattataacatcaaaggaattggtaag PRIMER_INTERNAL_5_SEQUENCE=tctcttcttgattcaatgaatctca PRIMER_LEFT_5=505,22 PRIMER_RIGHT_5=763,27 PRIMER_INTERNAL_5=554,25 PRIMER_LEFT_5_TM=60.503 PRIMER_RIGHT_5_TM=58.519 PRIMER_INTERNAL_5_TM=59.385 PRIMER_LEFT_5_GC_PERCENT=45.455 PRIMER_RIGHT_5_GC_PERCENT=29.630 PRIMER_INTERNAL_5_GC_PERCENT=32.000 PRIMER_LEFT_5_SELF_ANY=2.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_INTERNAL_5_SELF_ANY=11.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_INTERNAL_5_SELF_END=7.00 PRIMER_LEFT_5_END_STABILITY=6.6000 PRIMER_RIGHT_5_END_STABILITY=5.7000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PAIR_5_PRODUCT_SIZE=259 PRIMER_PAIR_6_PENALTY=11.966876 PRIMER_LEFT_6_PENALTY=9.464315 PRIMER_RIGHT_6_PENALTY=2.502561 PRIMER_INTERNAL_6_PENALTY=2.515892 PRIMER_LEFT_6_SEQUENCE=cataagaacattcattgacattttaag PRIMER_RIGHT_6_SEQUENCE=gctgatgaaggaaagcaagaag PRIMER_INTERNAL_6_SEQUENCE=actttgttccctccacattctc PRIMER_LEFT_6=229,27 PRIMER_RIGHT_6=527,22 PRIMER_INTERNAL_6=300,22 PRIMER_LEFT_6_TM=57.536 PRIMER_RIGHT_6_TM=60.503 PRIMER_INTERNAL_6_TM=59.484 PRIMER_LEFT_6_GC_PERCENT=25.926 PRIMER_RIGHT_6_GC_PERCENT=45.455 PRIMER_INTERNAL_6_GC_PERCENT=45.455 PRIMER_LEFT_6_SELF_ANY=7.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_INTERNAL_6_SELF_ANY=3.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_INTERNAL_6_SELF_END=0.00 PRIMER_LEFT_6_END_STABILITY=6.3000 PRIMER_RIGHT_6_END_STABILITY=6.7000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PAIR_6_PRODUCT_SIZE=299 PRIMER_PAIR_7_PENALTY=13.686032 PRIMER_LEFT_7_PENALTY=5.629294 PRIMER_RIGHT_7_PENALTY=8.056737 PRIMER_INTERNAL_7_PENALTY=0.833045 PRIMER_LEFT_7_SEQUENCE=tttgatgtcaacatcttcgtaaag PRIMER_RIGHT_7_SEQUENCE=ttgcagagactaatcaagactaaagag PRIMER_INTERNAL_7_SEQUENCE=tctcagattcgattctcccc PRIMER_LEFT_7=30,24 PRIMER_RIGHT_7=202,27 PRIMER_INTERNAL_7=55,20 PRIMER_LEFT_7_TM=58.371 PRIMER_RIGHT_7_TM=58.943 PRIMER_INTERNAL_7_TM=59.167 PRIMER_LEFT_7_GC_PERCENT=33.333 PRIMER_RIGHT_7_GC_PERCENT=37.037 PRIMER_INTERNAL_7_GC_PERCENT=50.000 PRIMER_LEFT_7_SELF_ANY=8.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_INTERNAL_7_SELF_ANY=8.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_INTERNAL_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=6.3000 PRIMER_RIGHT_7_END_STABILITY=6.7000 PRIMER_PAIR_7_COMPL_ANY=5.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PAIR_7_PRODUCT_SIZE=173 = primer3-2.3.6/test/primer_must_match_input0000664000077300007730000001561612231156245017732 0ustar ioanaioanaSEQUENCE_ID=Pick Primers as a reference SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=25 PRIMER_TASK=generic PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 = SEQUENCE_ID=Must obtain same result as test before SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=25 PRIMER_TASK=generic PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 PRIMER_MUST_MATCH_THREE_PRIME=nnnnn PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=nnnnn PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=nnnnn = SEQUENCE_ID=Pick probes without G at the 3 and 5 prime end SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=25 PRIMER_TASK=generic PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 PRIMER_MUST_MATCH_THREE_PRIME=nnnnn PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=nnnnh PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=hnnnn = SEQUENCE_ID=Pick probes without G at 5 nucleotides the 3 and 5 prime end SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=25 PRIMER_TASK=generic PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 PRIMER_MUST_MATCH_THREE_PRIME=nnnnn PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=hhhhh PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=hhhhh = SEQUENCE_ID=Primers must end in AG SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=25 PRIMER_TASK=generic PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 PRIMER_MUST_MATCH_THREE_PRIME=nnnAG PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn = primer3-2.3.6/test/long_seq_tm_test.pl0000664000077300007730000000065212231156245016743 0ustar ioanaioana#!/usr/local/bin/perl # Script that re-implements the calculation for long sequences. # Compare output to that of long_seq_tm_test.pl my ($seq, $start, $len) = @ARGV; my $s = substr($seq, $start, $len); my $GC_count = $s =~ tr/gGcC/gGcC/; $salt=50; $divalent=0; $dntp=0; $salt = $salt + 120*sqrt(($divalent-$dntp)); $r = log($salt/1000.0)/log(10); $tm = 81.5 + 16.6*$r + 41*$GC_count/$len - 600./$len; print "tm = $tm\n"; primer3-2.3.6/test/primer_internal_formatted_output0000664000077300007730000013574212231156245021653 0ustar ioanaioanaPRIMER PICKING RESULTS FOR boundary_test_00 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 35 33 0 0 0 0 0 0 0 0 0 0 0 2 Right 35 33 0 0 0 0 0 0 0 0 0 0 0 2 Intl 60 57 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 4, unacceptable product size 1, no internal oligo 2, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_01 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 29 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 30 28 0 0 0 0 0 0 0 0 0 0 0 2 Right 30 29 0 0 0 0 0 0 0 0 0 0 0 1 Intl 60 57 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 2, unacceptable product size 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_02 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 29 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Intl 60 57 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_05 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 29 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 0 NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Intl 60 57 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 4, unacceptable product size 3, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_11 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 0 NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACT 60 CACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 1 0 0 0 0 0 0 0 0 0 0 0 4 Intl 60 59 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 8, unacceptable product size 6, no internal oligo 2, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_11_2 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 24 60.81 31.82 12.00 11.50 CACGTCATCNNATCAAATTTGATT RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 29 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00 0 NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 3 24 60.81 31.82 12.00 11.00 ACGTCATCNNATCAAATTTGATTG RIGHT PRIMER 82 24 61.74 34.78 4.00 3.00 NGAATTGTTTGGAAATGACAGTGA INTERNAL OLIGO 29 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG PRODUCT SIZE: 80, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Intl 60 52 0 0 0 0 0 0 0 0 0 0 0 8 Pair Stats: considered 10, unacceptable product size 8, ok 2 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_12 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 24 60.81 31.82 12.00 11.50 CACGTCATCNNATCAAATTTGATT RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 29 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00 0 NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 3 24 60.81 31.82 12.00 11.00 ACGTCATCNNATCAAATTTGATTG RIGHT PRIMER 82 24 61.74 34.78 4.00 3.00 NGAATTGTTTGGAAATGACAGTGA INTERNAL OLIGO 29 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG PRODUCT SIZE: 80, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Intl 60 52 0 0 0 0 0 0 0 0 0 0 0 8 Pair Stats: considered 10, unacceptable product size 8, ok 2 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_13 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 24 54.69 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAA RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 31 25 56.20 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTNN SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 0 NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Intl 60 23 0 0 0 0 0 0 0 0 0 0 0 37 Pair Stats: considered 4, unacceptable product size 3, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_14 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 24 55.24 4.17 0.00 0.00 AAAAAAAAAAAAAAAAACAAAAAA RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 29 25 56.20 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTNN SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00 0 NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 2 0 0 0 0 0 0 0 0 0 0 0 3 Right 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Intl 60 25 0 0 0 0 0 0 0 0 0 0 0 35 Pair Stats: considered 6, unacceptable product size 5, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_15 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 24 89.31 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGG RIGHT PRIMER 81 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 32 25 55.84 0.00 0.00 0.00 TTTTTTTTTTTTTTTTTTTTTTTTT SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00 0 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACT >>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ << 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 2 0 0 0 0 0 0 0 0 0 0 0 3 Intl 60 25 0 0 0 0 0 0 0 0 0 0 0 35 Pair Stats: considered 6, unacceptable product size 5, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_16 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 0 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 8 0 0 0 0 0 0 0 0 0 0 0 2 Right 10 8 0 0 0 0 0 0 0 0 0 0 0 2 Intl 35 0 0 0 0 0 0 0 0 0 0 0 0 35 Pair Stats: considered 4, no internal oligo 4, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_17 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 0 NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACT 60 CACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 35 31 0 0 0 0 0 0 0 0 0 0 0 4 Right 35 31 0 0 0 0 0 0 0 0 0 0 0 4 Intl 60 0 0 0 0 29 0 0 0 0 0 0 0 31 Pair Stats: considered 16, unacceptable product size 4, no internal oligo 12, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_18 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 111 INCLUDED REGION SIZE: 111 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 10,50 0 NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGN xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 60 NNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN KEYS (in order of precedence): xxxxxx excluded region for internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 62 53 0 0 0 0 0 0 0 0 0 0 0 9 Right 62 53 0 0 0 0 0 0 0 0 0 0 0 9 Intl 87 39 0 40 0 0 0 0 0 0 0 0 0 8 Pair Stats: considered 81, unacceptable product size 49, no internal oligo 32, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_19 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 112 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT SEQUENCE SIZE: 115 INCLUDED REGION SIZE: 115 PRODUCT SIZE: 110, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 10,50 0 NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNN >>>>>>>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 60 CCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN ^^^^^^^^^^^^^^^^^^^^^^^^^ <<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 112 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT PRODUCT SIZE: 111, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 111 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT PRODUCT SIZE: 109, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 3 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 111 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT PRODUCT SIZE: 110, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 4 LEFT PRIMER 29 24 67.92 54.17 9.00 0.00 AAGCTAAGCTTGCCCGTATCCCCT RIGHT PRIMER 112 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT PRODUCT SIZE: 84, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00 5 LEFT PRIMER 29 24 67.92 54.17 9.00 0.00 AAGCTAAGCTTGCCCGTATCCCCT RIGHT PRIMER 111 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT PRODUCT SIZE: 83, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00 6 LEFT PRIMER 30 24 69.23 58.33 7.00 1.00 AGCTAAGCTTGCCCGTATCCCCTG RIGHT PRIMER 112 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 60 25 65.58 52.00 6.00 1.00 CCGTTAGGTGCTAGCCGTGTAGATT PRODUCT SIZE: 83, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 66 57 0 0 0 0 0 0 0 0 0 0 0 9 Right 66 57 0 0 0 0 0 0 0 0 0 0 0 9 Intl 91 84 0 5 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 20, unacceptable product size 3, no internal oligo 10, ok 7 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_20 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 251 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 75 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG SEQUENCE SIZE: 291 INCLUDED REGION SIZE: 250 PRODUCT SIZE: 249, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 5,50 60,15 0 NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATA .. >>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 60 CGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNN xxxxxxxxxxxxxxx^^^^^^^^^^^^^^^^^^^^^^^^^ 120 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 180 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATT <<<<<<<<<<<< 240 TCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT <<<<<<<<<<<<....................................... KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 251 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 75 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG PRODUCT SIZE: 250, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 50 24 60.30 50.00 8.00 6.00 GGAGACGATACGGTAAGCTAGCTA RIGHT PRIMER 251 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 75 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG PRODUCT SIZE: 202, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00 3 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 250 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 75 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 4 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 250 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 75 25 63.81 52.00 12.00 12.00 CTGGTCAAAGCTAGCGGTAGCTTAG PRODUCT SIZE: 249, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 151 99 0 0 0 0 0 0 0 0 0 0 0 52 Right 151 148 0 0 0 0 0 0 0 0 0 0 0 3 Intl 226 175 0 47 0 0 0 0 0 0 0 0 0 4 Pair Stats: considered 14, no internal oligo 8, ok 6 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_21 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 173 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 61 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA SEQUENCE SIZE: 176 INCLUDED REGION SIZE: 176 PRODUCT SIZE: 171, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >>>>>>>>>>>>>>>>>>>>>>>> 60 GAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG ^^^^^^^^^^^^^^^^^^^^^^^^^ 120 GGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 173 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 61 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA PRODUCT SIZE: 172, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 ACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 172 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 61 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA PRODUCT SIZE: 170, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 3 LEFT PRIMER 2 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 172 24 58.98 33.33 4.00 3.00 AATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 61 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA PRODUCT SIZE: 171, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 4 LEFT PRIMER 37 24 89.31 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGG RIGHT PRIMER 173 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG INTERNAL OLIGO 61 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA PRODUCT SIZE: 137, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 77 27 0 0 0 0 0 0 0 0 0 0 0 50 Right 77 28 0 0 0 0 0 0 0 0 0 0 0 49 Intl 152 57 0 0 0 0 0 1 93 0 0 0 0 1 Pair Stats: considered 323, unacceptable product size 223, no internal oligo 87, ok 13 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_22 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 84 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT >>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Intl 60 57 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_23 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 1,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_24 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 2,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..x>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_25 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 0,3 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..x>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_26 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 29,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> x^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_27 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 30,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT .. x 60 CACTGTCATTTCCAAACAATTCNN .. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, no internal oligo 1, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_28 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 54,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT .. x 60 CACTGTCATTTCCAAACAATTCNN .. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, no internal oligo 1, ok 0 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_29 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 55,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^x <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_30 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 81,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_31 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 82,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence xxxxxx excluded region for internal oligo >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_32 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions WARNING: PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 81,2 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_33 No mispriming library specified No internal oligo mishyb library specified Using 0-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 2 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 81 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT INTERNAL OLIGO 30 25 71.50 56.00 12.00 8.00 ACGTACGTACGTTGCAGTCGACGCA SEQUENCE SIZE: 84 INCLUDED REGION SIZE: 80 PRODUCT SIZE: 80, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 0 NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACT ..>>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^^^^^^^^^^^^^^^^^^^^^ <<< 60 CACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Intl 56 53 0 1 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR boundary_test_34 INPUT PROBLEM: Too many elements for tag SEQUENCE_INTERNAL_EXCLUDED_REGION primer3-2.3.6/test/primer_obj_fn_output0000664000077300007730000022246012231156245017221 0ustar ioanaioanaSEQUENCE_ID=io_compl PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 PRIMER_PAIR_WT_IO_PENALTY=1 PRIMER_PAIR_WT_PR_PENALTY=0 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=1 PRIMER_INTERNAL_WT_SELF_END=2 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG PRIMER_LEFT_EXPLAIN=considered 1290, GC content failed 10, low tm 60, high tm 1039, high any compl 1, high end compl 72, ok 108 PRIMER_RIGHT_EXPLAIN=considered 1290, GC content failed 32, low tm 38, high tm 1096, high any compl 1, high end compl 47, ok 76 PRIMER_INTERNAL_EXPLAIN=considered 1565, GC content failed 32, low tm 3, high tm 886, ok 644 PRIMER_PAIR_EXPLAIN=considered 10429, unacceptable product size 5893, high end compl 435, no internal oligo 767, ok 3334 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.000000 PRIMER_LEFT_0_PENALTY=0.000000 PRIMER_RIGHT_0_PENALTY=0.000000 PRIMER_INTERNAL_0_PENALTY=1.000000 PRIMER_LEFT_0_SEQUENCE=AAGACTCCGCCAGCCTTG PRIMER_RIGHT_0_SEQUENCE=CAGCCTGACGGTCATCAT PRIMER_INTERNAL_0_SEQUENCE=CCTCTCCCTCAACTCACC PRIMER_LEFT_0=56,18 PRIMER_RIGHT_0=118,18 PRIMER_INTERNAL_0=83,18 PRIMER_LEFT_0_TM=61.507 PRIMER_RIGHT_0_TM=58.043 PRIMER_INTERNAL_0_TM=55.425 PRIMER_LEFT_0_GC_PERCENT=61.111 PRIMER_RIGHT_0_GC_PERCENT=55.556 PRIMER_INTERNAL_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=1.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=6.5000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=63 PRIMER_PAIR_1_PENALTY=1.000000 PRIMER_LEFT_1_PENALTY=0.000000 PRIMER_RIGHT_1_PENALTY=0.000000 PRIMER_INTERNAL_1_PENALTY=1.000000 PRIMER_LEFT_1_SEQUENCE=AAGACTCCGCCAGCCTTG PRIMER_RIGHT_1_SEQUENCE=TCAGCCTGACGGTCATCAT PRIMER_INTERNAL_1_SEQUENCE=CCTCTCCCTCAACTCACC PRIMER_LEFT_1=56,18 PRIMER_RIGHT_1=119,19 PRIMER_INTERNAL_1=83,18 PRIMER_LEFT_1_TM=61.507 PRIMER_RIGHT_1_TM=60.222 PRIMER_INTERNAL_1_TM=55.425 PRIMER_LEFT_1_GC_PERCENT=61.111 PRIMER_RIGHT_1_GC_PERCENT=52.632 PRIMER_INTERNAL_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_INTERNAL_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=6.5000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=64 PRIMER_PAIR_2_PENALTY=1.000000 PRIMER_LEFT_2_PENALTY=0.000000 PRIMER_RIGHT_2_PENALTY=0.000000 PRIMER_INTERNAL_2_PENALTY=1.000000 PRIMER_LEFT_2_SEQUENCE=AAGACTCCGCCAGCCTTG PRIMER_RIGHT_2_SEQUENCE=CTCAGCCTGACGGTCATC PRIMER_INTERNAL_2_SEQUENCE=CTCTCCCTCAACTCACCA PRIMER_LEFT_2=56,18 PRIMER_RIGHT_2=120,18 PRIMER_INTERNAL_2=84,18 PRIMER_LEFT_2_TM=61.507 PRIMER_RIGHT_2_TM=57.723 PRIMER_INTERNAL_2_TM=54.310 PRIMER_LEFT_2_GC_PERCENT=61.111 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_INTERNAL_2_GC_PERCENT=55.556 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_INTERNAL_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=65 PRIMER_PAIR_3_PENALTY=1.000000 PRIMER_LEFT_3_PENALTY=0.000000 PRIMER_RIGHT_3_PENALTY=0.000000 PRIMER_INTERNAL_3_PENALTY=1.000000 PRIMER_LEFT_3_SEQUENCE=AAGACTCCGCCAGCCTTG PRIMER_RIGHT_3_SEQUENCE=CTCAGCCTGACGGTCATCAT PRIMER_INTERNAL_3_SEQUENCE=CCTCTCCCTCAACTCACC PRIMER_LEFT_3=56,18 PRIMER_RIGHT_3=120,20 PRIMER_INTERNAL_3=83,18 PRIMER_LEFT_3_TM=61.507 PRIMER_RIGHT_3_TM=61.251 PRIMER_INTERNAL_3_TM=55.425 PRIMER_LEFT_3_GC_PERCENT=61.111 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_GC_PERCENT=61.111 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_INTERNAL_3_SELF_ANY=1.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=6.5000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=65 PRIMER_PAIR_4_PENALTY=1.000000 PRIMER_LEFT_4_PENALTY=0.000000 PRIMER_RIGHT_4_PENALTY=0.000000 PRIMER_INTERNAL_4_PENALTY=1.000000 PRIMER_LEFT_4_SEQUENCE=AAGACTCCGCCAGCCTTG PRIMER_RIGHT_4_SEQUENCE=CCTCAGCCTGACGGTCATC PRIMER_INTERNAL_4_SEQUENCE=CTCTCCCTCAACTCACCA PRIMER_LEFT_4=56,18 PRIMER_RIGHT_4=121,19 PRIMER_INTERNAL_4=84,18 PRIMER_LEFT_4_TM=61.507 PRIMER_RIGHT_4_TM=61.836 PRIMER_INTERNAL_4_TM=54.310 PRIMER_LEFT_4_GC_PERCENT=61.111 PRIMER_RIGHT_4_GC_PERCENT=63.158 PRIMER_INTERNAL_4_GC_PERCENT=55.556 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_INTERNAL_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=66 = SEQUENCE_ID=diff_tm PRIMER_PAIR_WT_IO_PENALTY=0 PRIMER_PAIR_WT_PR_PENALTY=1 PRIMER_PAIR_WT_DIFF_TM=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_TASK=pick_pcr_primers SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG PRIMER_LEFT_EXPLAIN=considered 1290, GC content failed 10, low tm 60, high tm 1039, high any compl 1, high end compl 72, ok 108 PRIMER_RIGHT_EXPLAIN=considered 1290, GC content failed 32, low tm 38, high tm 1096, high any compl 1, high end compl 47, ok 76 PRIMER_PAIR_EXPLAIN=considered 10429, unacceptable product size 5893, high end compl 435, ok 4101 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.000039 PRIMER_LEFT_0_PENALTY=0.000000 PRIMER_RIGHT_0_PENALTY=0.000000 PRIMER_LEFT_0_SEQUENCE=AGCCGAGGATGACTTCACTTTC PRIMER_RIGHT_0_SEQUENCE=CATGGTGAGTTGAGGGAGAGG PRIMER_LEFT_0=1,22 PRIMER_RIGHT_0=103,21 PRIMER_LEFT_0_TM=62.010 PRIMER_RIGHT_0_TM=62.010 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=57.143 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=103 PRIMER_PAIR_1_PENALTY=0.005276 PRIMER_LEFT_1_PENALTY=0.000000 PRIMER_RIGHT_1_PENALTY=0.000000 PRIMER_LEFT_1_SEQUENCE=AAGCCGAGGATGACTTCACTTT PRIMER_RIGHT_1_SEQUENCE=TCATCATGGTGAGTTGAGGGA PRIMER_LEFT_1=0,22 PRIMER_RIGHT_1=107,21 PRIMER_LEFT_1_TM=61.489 PRIMER_RIGHT_1_TM=61.484 PRIMER_LEFT_1_GC_PERCENT=45.455 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=108 PRIMER_PAIR_2_PENALTY=0.005368 PRIMER_LEFT_2_PENALTY=0.000000 PRIMER_RIGHT_2_PENALTY=0.000000 PRIMER_LEFT_2_SEQUENCE=ACGAGTGAAGACTCCGCCA PRIMER_RIGHT_2_SEQUENCE=CATGGTGAGTTGAGGGAGAGG PRIMER_LEFT_2=49,19 PRIMER_RIGHT_2=103,21 PRIMER_LEFT_2_TM=62.005 PRIMER_RIGHT_2_TM=62.010 PRIMER_LEFT_2_GC_PERCENT=57.895 PRIMER_RIGHT_2_GC_PERCENT=57.143 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=11.7000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=55 PRIMER_PAIR_3_PENALTY=0.005368 PRIMER_LEFT_3_PENALTY=0.000000 PRIMER_RIGHT_3_PENALTY=0.000000 PRIMER_LEFT_3_SEQUENCE=CACGAGTGAAGACTCCGCC PRIMER_RIGHT_3_SEQUENCE=CATGGTGAGTTGAGGGAGAGG PRIMER_LEFT_3=48,19 PRIMER_RIGHT_3=103,21 PRIMER_LEFT_3_TM=62.005 PRIMER_RIGHT_3_TM=62.010 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=57.143 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=12.9000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=56 PRIMER_PAIR_4_PENALTY=0.005368 PRIMER_LEFT_4_PENALTY=0.000000 PRIMER_RIGHT_4_PENALTY=0.000000 PRIMER_LEFT_4_SEQUENCE=CCACGAGTGAAGACTCCGC PRIMER_RIGHT_4_SEQUENCE=CATGGTGAGTTGAGGGAGAGG PRIMER_LEFT_4=47,19 PRIMER_RIGHT_4=103,21 PRIMER_LEFT_4_TM=62.005 PRIMER_RIGHT_4_TM=62.010 PRIMER_LEFT_4_GC_PERCENT=63.158 PRIMER_RIGHT_4_GC_PERCENT=57.143 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=11.4000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=57 = SEQUENCE_ID=num_NS PRIMER_PAIR_WT_DIFF_TM=0 PRIMER_WT_NUM_NS=1 PRIMER_MAX_NS_ACCEPTED=15 SEQUENCE_TEMPLATE=AAGCCGNGGATGANTTCACTTTCNGACCNTGCAGAGCCGANTTTCACNCACGAGNGAAGACTNCGCCAGCCTTGCCCCTNGGGCCTCTNCCTCAACTCNCCATGATGNCCGTCAGGNNNNNNNNNCAGGCGNACTGAGGCGTGGTNCGCGGGGCTCAGGNNNNNNNNNCCTGCGCCGG PRIMER_LEFT_EXPLAIN=considered 1093, GC content failed 4, low tm 61, high tm 818, high end compl 20, long poly-x seq 27, ok 163 PRIMER_RIGHT_EXPLAIN=considered 1000, GC content failed 67, low tm 30, high tm 741, high end compl 10, long poly-x seq 44, ok 108 PRIMER_PAIR_EXPLAIN=considered 701, unacceptable product size 590, ok 111 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.000000 PRIMER_LEFT_0_PENALTY=1.000000 PRIMER_RIGHT_0_PENALTY=1.000000 PRIMER_LEFT_0_SEQUENCE=TGCAGAGCCGANTTTCAC PRIMER_RIGHT_0_SEQUENCE=GAGTTGAGGNAGAGGCCC PRIMER_LEFT_0=29,18 PRIMER_RIGHT_0=97,18 PRIMER_LEFT_0_TM=58.508 PRIMER_RIGHT_0_TM=57.993 PRIMER_LEFT_0_GC_PERCENT=52.941 PRIMER_RIGHT_0_GC_PERCENT=64.706 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=12.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.50 PRIMER_PAIR_0_PRODUCT_SIZE=69 PRIMER_PAIR_1_PENALTY=3.000000 PRIMER_LEFT_1_PENALTY=1.000000 PRIMER_RIGHT_1_PENALTY=2.000000 PRIMER_LEFT_1_SEQUENCE=GGGCCTCTNCCTCAACTC PRIMER_RIGHT_1_SEQUENCE=GNACCACGCCTCAGTNCG PRIMER_LEFT_1=80,18 PRIMER_RIGHT_1=146,18 PRIMER_LEFT_1_TM=57.993 PRIMER_RIGHT_1_TM=61.904 PRIMER_LEFT_1_GC_PERCENT=64.706 PRIMER_RIGHT_1_GC_PERCENT=68.750 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.50 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=8.7500 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=67 PRIMER_PAIR_2_PENALTY=3.000000 PRIMER_LEFT_2_PENALTY=1.000000 PRIMER_RIGHT_2_PENALTY=2.000000 PRIMER_LEFT_2_SEQUENCE=GGGCCTCTNCCTCAACTC PRIMER_RIGHT_2_SEQUENCE=CGNACCACGCCTCAGTNC PRIMER_LEFT_2=80,18 PRIMER_RIGHT_2=147,18 PRIMER_LEFT_2_TM=57.993 PRIMER_RIGHT_2_TM=61.904 PRIMER_LEFT_2_GC_PERCENT=64.706 PRIMER_RIGHT_2_GC_PERCENT=68.750 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.50 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.7500 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.75 PRIMER_PAIR_2_PRODUCT_SIZE=68 PRIMER_PAIR_3_PENALTY=3.000000 PRIMER_LEFT_3_PENALTY=1.000000 PRIMER_RIGHT_3_PENALTY=2.000000 PRIMER_LEFT_3_SEQUENCE=AAGACTNCGCCAGCCTTG PRIMER_RIGHT_3_SEQUENCE=ATCATGGNGAGTTGAGGNAG PRIMER_LEFT_3=56,18 PRIMER_RIGHT_3=105,20 PRIMER_LEFT_3_TM=59.835 PRIMER_RIGHT_3_TM=57.730 PRIMER_LEFT_3_GC_PERCENT=58.824 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.50 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=8.5500 PRIMER_PAIR_3_COMPL_ANY=4.75 PRIMER_PAIR_3_COMPL_END=0.75 PRIMER_PAIR_3_PRODUCT_SIZE=50 PRIMER_PAIR_4_PENALTY=3.000000 PRIMER_LEFT_4_PENALTY=1.000000 PRIMER_RIGHT_4_PENALTY=2.000000 PRIMER_LEFT_4_SEQUENCE=AAGACTNCGCCAGCCTTG PRIMER_RIGHT_4_SEQUENCE=ATCATGGNGAGTTGAGGNAGA PRIMER_LEFT_4=56,18 PRIMER_RIGHT_4=105,21 PRIMER_LEFT_4_TM=59.835 PRIMER_RIGHT_4_TM=59.709 PRIMER_LEFT_4_GC_PERCENT=58.824 PRIMER_RIGHT_4_GC_PERCENT=47.368 PRIMER_LEFT_4_SELF_ANY=5.50 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=7.0500 PRIMER_PAIR_4_COMPL_ANY=4.75 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=50 = SEQUENCE_ID=product_size PRIMER_WT_NUM_NS=0 PRIMER_PRODUCT_OPT_SIZE=60 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=1 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PAIR_WT_DIFF_TM=0 PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_WT_LIBRARY_MISPRIMING=0 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 PRIMER_PAIR_MAX_DIFF_TM=3 PRIMER_MISPRIMING_LIBRARY= PRIMER_LEFT_EXPLAIN=considered 310, low tm 2, high tm 126, ok 182 PRIMER_RIGHT_EXPLAIN=considered 310, high tm 144, ok 166 PRIMER_PAIR_EXPLAIN=considered 607, unacceptable product size 70, tm diff too large 324, ok 213 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.000000 PRIMER_LEFT_0_PENALTY=0.000000 PRIMER_RIGHT_0_PENALTY=0.000000 PRIMER_LEFT_0_SEQUENCE=ACTTTCCGACCCTGCAGAGCC PRIMER_RIGHT_0_SEQUENCE=GGGCAAGGCTGGCGGAGT PRIMER_LEFT_0=17,21 PRIMER_RIGHT_0=76,18 PRIMER_LEFT_0_TM=67.188 PRIMER_RIGHT_0_TM=68.249 PRIMER_LEFT_0_GC_PERCENT=61.905 PRIMER_RIGHT_0_GC_PERCENT=72.222 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=60 PRIMER_PAIR_1_PENALTY=0.000000 PRIMER_LEFT_1_PENALTY=0.000000 PRIMER_RIGHT_1_PENALTY=0.000000 PRIMER_LEFT_1_SEQUENCE=ACTTTCCGACCCTGCAGAGCC PRIMER_RIGHT_1_SEQUENCE=GGGCAAGGCTGGCGGAGTC PRIMER_LEFT_1=17,21 PRIMER_RIGHT_1=76,19 PRIMER_LEFT_1_TM=67.188 PRIMER_RIGHT_1_TM=69.823 PRIMER_LEFT_1_GC_PERCENT=61.905 PRIMER_RIGHT_1_GC_PERCENT=73.684 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=60 PRIMER_PAIR_2_PENALTY=0.000000 PRIMER_LEFT_2_PENALTY=0.000000 PRIMER_RIGHT_2_PENALTY=0.000000 PRIMER_LEFT_2_SEQUENCE=CACTTTCCGACCCTGCAGAGC PRIMER_RIGHT_2_SEQUENCE=GGCAAGGCTGGCGGAGTC PRIMER_LEFT_2=16,21 PRIMER_RIGHT_2=75,18 PRIMER_LEFT_2_TM=66.655 PRIMER_RIGHT_2_TM=66.706 PRIMER_LEFT_2_GC_PERCENT=61.905 PRIMER_RIGHT_2_GC_PERCENT=72.222 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=60 PRIMER_PAIR_3_PENALTY=0.000000 PRIMER_LEFT_3_PENALTY=0.000000 PRIMER_RIGHT_3_PENALTY=0.000000 PRIMER_LEFT_3_SEQUENCE=CACTTTCCGACCCTGCAGAGCC PRIMER_RIGHT_3_SEQUENCE=GGCAAGGCTGGCGGAGTC PRIMER_LEFT_3=16,22 PRIMER_RIGHT_3=75,18 PRIMER_LEFT_3_TM=69.546 PRIMER_RIGHT_3_TM=66.706 PRIMER_LEFT_3_GC_PERCENT=63.636 PRIMER_RIGHT_3_GC_PERCENT=72.222 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=5.00 PRIMER_PAIR_3_PRODUCT_SIZE=60 PRIMER_PAIR_4_PENALTY=0.000000 PRIMER_LEFT_4_PENALTY=0.000000 PRIMER_RIGHT_4_PENALTY=0.000000 PRIMER_LEFT_4_SEQUENCE=CACTTTCCGACCCTGCAGAGC PRIMER_RIGHT_4_SEQUENCE=GGCAAGGCTGGCGGAGTCT PRIMER_LEFT_4=16,21 PRIMER_RIGHT_4=75,19 PRIMER_LEFT_4_TM=66.655 PRIMER_RIGHT_4_TM=67.348 PRIMER_LEFT_4_GC_PERCENT=61.905 PRIMER_RIGHT_4_GC_PERCENT=68.421 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=6.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=60 = SEQUENCE_ID=rep_sim PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0 PRIMER_MAX_LIBRARY_MISPRIMING=20 PRIMER_WT_LIBRARY_MISPRIMING=1 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC PRIMER_LEFT_EXPLAIN=considered 310, low tm 2, high tm 126, ok 182 PRIMER_RIGHT_EXPLAIN=considered 310, high tm 144, ok 166 PRIMER_PAIR_EXPLAIN=considered 624, unacceptable product size 163, tm diff too large 320, ok 141 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=14.000000 PRIMER_LEFT_0_PENALTY=7.000000 PRIMER_RIGHT_0_PENALTY=7.000000 PRIMER_LEFT_0_SEQUENCE=GGATGACTTCACTTTCCGACC PRIMER_RIGHT_0_SEQUENCE=GGCTGGCGGAGTCTTCAC PRIMER_LEFT_0=7,21 PRIMER_RIGHT_0=70,18 PRIMER_LEFT_0_TM=60.878 PRIMER_RIGHT_0_TM=61.981 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=7.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_0_LIBRARY_MISPRIMING=14.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=9.6000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=64 PRIMER_PAIR_1_PENALTY=14.000000 PRIMER_LEFT_1_PENALTY=7.000000 PRIMER_RIGHT_1_PENALTY=7.000000 PRIMER_LEFT_1_SEQUENCE=GGATGACTTCACTTTCCGACC PRIMER_RIGHT_1_SEQUENCE=AGGCTGGCGGAGTCTTCA PRIMER_LEFT_1=7,21 PRIMER_RIGHT_1=71,18 PRIMER_LEFT_1_TM=60.878 PRIMER_RIGHT_1_TM=62.110 PRIMER_LEFT_1_GC_PERCENT=52.381 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=14.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=65 PRIMER_PAIR_2_PENALTY=14.000000 PRIMER_LEFT_2_PENALTY=7.000000 PRIMER_RIGHT_2_PENALTY=7.000000 PRIMER_LEFT_2_SEQUENCE=GGATGACTTCACTTTCCGACC PRIMER_RIGHT_2_SEQUENCE=AGGCTGGCGGAGTCTTCAC PRIMER_LEFT_2=7,21 PRIMER_RIGHT_2=71,19 PRIMER_LEFT_2_TM=60.878 PRIMER_RIGHT_2_TM=62.895 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=63.158 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=7.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_2_LIBRARY_MISPRIMING=14.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=9.6000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=65 PRIMER_PAIR_3_PENALTY=14.000000 PRIMER_LEFT_3_PENALTY=7.000000 PRIMER_RIGHT_3_PENALTY=7.000000 PRIMER_LEFT_3_SEQUENCE=GGATGACTTCACTTTCCGACC PRIMER_RIGHT_3_SEQUENCE=AAGGCTGGCGGAGTCTTCA PRIMER_LEFT_3=7,21 PRIMER_RIGHT_3=72,19 PRIMER_LEFT_3_TM=60.878 PRIMER_RIGHT_3_TM=63.377 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=57.895 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_3_LIBRARY_MISPRIMING=14.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=9.6000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=66 PRIMER_PAIR_4_PENALTY=15.000000 PRIMER_LEFT_4_PENALTY=8.000000 PRIMER_RIGHT_4_PENALTY=7.000000 PRIMER_LEFT_4_SEQUENCE=TTTCCGACCCTGCAGAGC PRIMER_RIGHT_4_SEQUENCE=GGCTGGCGGAGTCTTCAC PRIMER_LEFT_4=19,18 PRIMER_RIGHT_4=70,18 PRIMER_LEFT_4_TM=62.504 PRIMER_RIGHT_4_TM=61.981 PRIMER_LEFT_4_GC_PERCENT=61.111 PRIMER_RIGHT_4_GC_PERCENT=66.667 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=7.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_4_LIBRARY_MISPRIMING=15.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=52 = SEQUENCE_ID=pair_rep_sim PRIMER_WT_LIBRARY_MISPRIMING=0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTC PRIMER_LEFT_EXPLAIN=considered 310, low tm 2, high tm 126, ok 182 PRIMER_RIGHT_EXPLAIN=considered 310, high tm 144, ok 166 PRIMER_PAIR_EXPLAIN=considered 30212, unacceptable product size 14224, tm diff too large 10477, ok 5511 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=14.000000 PRIMER_LEFT_0_PENALTY=0.000000 PRIMER_RIGHT_0_PENALTY=0.000000 PRIMER_LEFT_0_SEQUENCE=TTTCCGACCCTGCAGAGC PRIMER_RIGHT_0_SEQUENCE=CTGGCGGAGTCTTCACTCG PRIMER_LEFT_0=19,18 PRIMER_RIGHT_0=68,19 PRIMER_LEFT_0_TM=62.504 PRIMER_RIGHT_0_TM=62.132 PRIMER_LEFT_0_GC_PERCENT=61.111 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_0_LIBRARY_MISPRIMING=14.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=50 PRIMER_PAIR_1_PENALTY=14.000000 PRIMER_LEFT_1_PENALTY=0.000000 PRIMER_RIGHT_1_PENALTY=0.000000 PRIMER_LEFT_1_SEQUENCE=TTTCCGACCCTGCAGAGC PRIMER_RIGHT_1_SEQUENCE=AGGCTGGCGGAGTCTTCA PRIMER_LEFT_1=19,18 PRIMER_RIGHT_1=71,18 PRIMER_LEFT_1_TM=62.504 PRIMER_RIGHT_1_TM=62.110 PRIMER_LEFT_1_GC_PERCENT=61.111 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=14.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=53 PRIMER_PAIR_2_PENALTY=14.000000 PRIMER_LEFT_2_PENALTY=0.000000 PRIMER_RIGHT_2_PENALTY=0.000000 PRIMER_LEFT_2_SEQUENCE=CTTTCCGACCCTGCAGAGC PRIMER_RIGHT_2_SEQUENCE=TGGCGGAGTCTTCACTCG PRIMER_LEFT_2=18,19 PRIMER_RIGHT_2=67,18 PRIMER_LEFT_2_TM=63.377 PRIMER_RIGHT_2_TM=61.148 PRIMER_LEFT_2_GC_PERCENT=63.158 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_2_LIBRARY_MISPRIMING=14.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=8.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=50 PRIMER_PAIR_3_PENALTY=14.000000 PRIMER_LEFT_3_PENALTY=0.000000 PRIMER_RIGHT_3_PENALTY=0.000000 PRIMER_LEFT_3_SEQUENCE=CTTTCCGACCCTGCAGAGC PRIMER_RIGHT_3_SEQUENCE=CTGGCGGAGTCTTCACTCG PRIMER_LEFT_3=18,19 PRIMER_RIGHT_3=68,19 PRIMER_LEFT_3_TM=63.377 PRIMER_RIGHT_3_TM=62.132 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=63.158 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_3_LIBRARY_MISPRIMING=14.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=51 PRIMER_PAIR_4_PENALTY=14.000000 PRIMER_LEFT_4_PENALTY=0.000000 PRIMER_RIGHT_4_PENALTY=0.000000 PRIMER_LEFT_4_SEQUENCE=CTTTCCGACCCTGCAGAGC PRIMER_RIGHT_4_SEQUENCE=GCTGGCGGAGTCTTCACTCG PRIMER_LEFT_4=18,19 PRIMER_RIGHT_4=69,20 PRIMER_LEFT_4_TM=63.377 PRIMER_RIGHT_4_TM=65.780 PRIMER_LEFT_4_GC_PERCENT=63.158 PRIMER_RIGHT_4_GC_PERCENT=65.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_PAIR_4_LIBRARY_MISPRIMING=14.00, ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=52 = SEQUENCE_ID=product_tm PRIMER_PAIR_WT_PRODUCT_TM_LT=1 PRIMER_PAIR_WT_PRODUCT_TM_GT=1 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0 PRIMER_PRODUCT_OPT_TM=80 PRIMER_MISPRIMING_LIBRARY= SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCT PRIMER_LEFT_EXPLAIN=considered 370, low tm 2, high tm 145, ok 223 PRIMER_RIGHT_EXPLAIN=considered 370, GC content failed 6, high tm 198, ok 166 PRIMER_PAIR_EXPLAIN=considered 37018, unacceptable product size 21030, tm diff too large 10477, ok 5511 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.950431 PRIMER_LEFT_0_PENALTY=0.000000 PRIMER_RIGHT_0_PENALTY=0.000000 PRIMER_LEFT_0_SEQUENCE=GCCGAGGATGACTTCACTTTCCG PRIMER_RIGHT_0_SEQUENCE=GGGCAAGGCTGGCGGAGT PRIMER_LEFT_0=2,23 PRIMER_RIGHT_0=76,18 PRIMER_LEFT_0_TM=68.296 PRIMER_RIGHT_0_TM=68.249 PRIMER_LEFT_0_GC_PERCENT=56.522 PRIMER_RIGHT_0_GC_PERCENT=72.222 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=10.2000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 PRIMER_PAIR_1_PENALTY=2.950431 PRIMER_LEFT_1_PENALTY=0.000000 PRIMER_RIGHT_1_PENALTY=0.000000 PRIMER_LEFT_1_SEQUENCE=GCCGAGGATGACTTCACTTTCCG PRIMER_RIGHT_1_SEQUENCE=GGGCAAGGCTGGCGGAGTC PRIMER_LEFT_1=2,23 PRIMER_RIGHT_1=76,19 PRIMER_LEFT_1_TM=68.296 PRIMER_RIGHT_1_TM=69.823 PRIMER_LEFT_1_GC_PERCENT=56.522 PRIMER_RIGHT_1_GC_PERCENT=73.684 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=10.2000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=75 PRIMER_PAIR_2_PENALTY=2.950431 PRIMER_LEFT_2_PENALTY=0.000000 PRIMER_RIGHT_2_PENALTY=0.000000 PRIMER_LEFT_2_SEQUENCE=GCCGAGGATGACTTCACTTTCCGA PRIMER_RIGHT_2_SEQUENCE=GGGCAAGGCTGGCGGAGT PRIMER_LEFT_2=2,24 PRIMER_RIGHT_2=76,18 PRIMER_LEFT_2_TM=69.669 PRIMER_RIGHT_2_TM=68.249 PRIMER_LEFT_2_GC_PERCENT=54.167 PRIMER_RIGHT_2_GC_PERCENT=72.222 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.9000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=75 PRIMER_PAIR_3_PENALTY=2.950431 PRIMER_LEFT_3_PENALTY=0.000000 PRIMER_RIGHT_3_PENALTY=0.000000 PRIMER_LEFT_3_SEQUENCE=GCCGAGGATGACTTCACTTTCCGA PRIMER_RIGHT_3_SEQUENCE=GGGCAAGGCTGGCGGAGTC PRIMER_LEFT_3=2,24 PRIMER_RIGHT_3=76,19 PRIMER_LEFT_3_TM=69.669 PRIMER_RIGHT_3_TM=69.823 PRIMER_LEFT_3_GC_PERCENT=54.167 PRIMER_RIGHT_3_GC_PERCENT=73.684 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.9000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=75 PRIMER_PAIR_4_PENALTY=3.176045 PRIMER_LEFT_4_PENALTY=0.000000 PRIMER_RIGHT_4_PENALTY=0.000000 PRIMER_LEFT_4_SEQUENCE=AGCCGAGGATGACTTCACTTTCCG PRIMER_RIGHT_4_SEQUENCE=GGGCAAGGCTGGCGGAGT PRIMER_LEFT_4=1,24 PRIMER_RIGHT_4=76,18 PRIMER_LEFT_4_TM=68.778 PRIMER_RIGHT_4_TM=68.249 PRIMER_LEFT_4_GC_PERCENT=54.167 PRIMER_RIGHT_4_GC_PERCENT=72.222 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=10.2000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=76 = SEQUENCE_ID=primer_compl PRIMER_MIN_GC=1.0 PRIMER_MAX_GC=99.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0 PRIMER_PRODUCT_OPT_SIZE=200 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0 PRIMER_PRODUCT_OPT_TM=0 PRIMER_WT_LIBRARY_MISPRIMING=0 PRIMER_WT_SELF_ANY=1 PRIMER_WT_SELF_END=2 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAATAGACATCATAATTAGATACAC PRIMER_LEFT_EXPLAIN=considered 814, low tm 177, long poly-x seq 23, ok 614 PRIMER_RIGHT_EXPLAIN=considered 940, low tm 244, ok 696 PRIMER_PAIR_EXPLAIN=considered 60, unacceptable product size 33, tm diff too large 10, ok 17 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.000000 PRIMER_LEFT_0_PENALTY=2.000000 PRIMER_RIGHT_0_PENALTY=2.000000 PRIMER_LEFT_0_SEQUENCE=AAGGTAAAAATGAGGTATGAA PRIMER_RIGHT_0_SEQUENCE=TGTCTATTTGTTGGAGGAA PRIMER_LEFT_0=68,21 PRIMER_RIGHT_0=126,19 PRIMER_LEFT_0_TM=50.931 PRIMER_RIGHT_0_TM=51.365 PRIMER_LEFT_0_GC_PERCENT=28.571 PRIMER_RIGHT_0_GC_PERCENT=36.842 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=59 PRIMER_PAIR_1_PENALTY=4.000000 PRIMER_LEFT_1_PENALTY=2.000000 PRIMER_RIGHT_1_PENALTY=2.000000 PRIMER_LEFT_1_SEQUENCE=AAGGTAAAAATGAGGTATGAA PRIMER_RIGHT_1_SEQUENCE=ATGTCTATTTGTTGGAGGA PRIMER_LEFT_1=68,21 PRIMER_RIGHT_1=127,19 PRIMER_LEFT_1_TM=50.931 PRIMER_RIGHT_1_TM=50.179 PRIMER_LEFT_1_GC_PERCENT=28.571 PRIMER_RIGHT_1_GC_PERCENT=36.842 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=60 PRIMER_PAIR_2_PENALTY=4.000000 PRIMER_LEFT_2_PENALTY=2.000000 PRIMER_RIGHT_2_PENALTY=2.000000 PRIMER_LEFT_2_SEQUENCE=AAGGTAAAAATGAGGTATGAA PRIMER_RIGHT_2_SEQUENCE=ATGTCTATTTGTTGGAGGAA PRIMER_LEFT_2=68,21 PRIMER_RIGHT_2=127,20 PRIMER_LEFT_2_TM=50.931 PRIMER_RIGHT_2_TM=52.153 PRIMER_LEFT_2_GC_PERCENT=28.571 PRIMER_RIGHT_2_GC_PERCENT=35.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=60 PRIMER_PAIR_3_PENALTY=4.000000 PRIMER_LEFT_3_PENALTY=2.000000 PRIMER_RIGHT_3_PENALTY=2.000000 PRIMER_LEFT_3_SEQUENCE=AAGGTAAAAATGAGGTATGAA PRIMER_RIGHT_3_SEQUENCE=GATGTCTATTTGTTGGAGG PRIMER_LEFT_3=68,21 PRIMER_RIGHT_3=128,19 PRIMER_LEFT_3_TM=50.931 PRIMER_RIGHT_3_TM=50.179 PRIMER_LEFT_3_GC_PERCENT=28.571 PRIMER_RIGHT_3_GC_PERCENT=42.105 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=61 PRIMER_PAIR_4_PENALTY=4.000000 PRIMER_LEFT_4_PENALTY=2.000000 PRIMER_RIGHT_4_PENALTY=2.000000 PRIMER_LEFT_4_SEQUENCE=AAGGTAAAAATGAGGTATGAA PRIMER_RIGHT_4_SEQUENCE=GATGTCTATTTGTTGGAGGA PRIMER_LEFT_4=68,21 PRIMER_RIGHT_4=128,20 PRIMER_LEFT_4_TM=50.931 PRIMER_RIGHT_4_TM=52.504 PRIMER_LEFT_4_GC_PERCENT=28.571 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=61 = PRIMER_MISPRIMING_LIBRARY= PRIMER_INTERNAL_MISHYB_LIBRARY=not_for_production.lib PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_WT_SELF_ANY=0 PRIMER_WT_SELF_END=0 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=1 PRIMER_PAIR_WT_COMPL_ANY=0 PRIMER_PAIR_WT_COMPL_END=0 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=20 PRIMER_PAIR_WT_PR_PENALTY=0 PRIMER_PAIR_WT_IO_PENALTY=1 SEQUENCE_ID=io_rep_sim SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATAACCTATACATAATGTGAAATATAATTTTT PRIMER_LEFT_EXPLAIN=considered 584, low tm 82, long poly-x seq 23, ok 479 PRIMER_RIGHT_EXPLAIN=considered 710, low tm 257, ok 453 PRIMER_INTERNAL_EXPLAIN=considered 859, GC content failed 67, low tm 223, long poly-x seq 23, ok 546 PRIMER_PAIR_EXPLAIN=considered 216987, unacceptable product size 157643, tm diff too large 48119, no internal oligo 8093, ok 3132 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=8.000000 PRIMER_LEFT_0_PENALTY=0.000000 PRIMER_RIGHT_0_PENALTY=0.000000 PRIMER_INTERNAL_0_PENALTY=8.000000 PRIMER_LEFT_0_SEQUENCE=GCCCAAATCCCATTATTC PRIMER_RIGHT_0_SEQUENCE=ATGTATAGGTTATTCATACCTCATT PRIMER_INTERNAL_0_SEQUENCE=ATAATGCATTTCAAGGTAAA PRIMER_LEFT_0=38,18 PRIMER_RIGHT_0=100,25 PRIMER_INTERNAL_0=56,20 PRIMER_LEFT_0_TM=55.228 PRIMER_RIGHT_0_TM=52.436 PRIMER_INTERNAL_0_TM=50.207 PRIMER_LEFT_0_GC_PERCENT=44.444 PRIMER_RIGHT_0_GC_PERCENT=28.000 PRIMER_INTERNAL_0_GC_PERCENT=25.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_INTERNAL_0_LIBRARY_MISHYB=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=63 PRIMER_PAIR_1_PENALTY=8.000000 PRIMER_LEFT_1_PENALTY=0.000000 PRIMER_RIGHT_1_PENALTY=0.000000 PRIMER_INTERNAL_1_PENALTY=8.000000 PRIMER_LEFT_1_SEQUENCE=GCCCAAATCCCATTATTC PRIMER_RIGHT_1_SEQUENCE=TATGTATAGGTTATTCATACCTCATT PRIMER_INTERNAL_1_SEQUENCE=ATAATGCATTTCAAGGTAAA PRIMER_LEFT_1=38,18 PRIMER_RIGHT_1=101,26 PRIMER_INTERNAL_1=56,20 PRIMER_LEFT_1_TM=55.228 PRIMER_RIGHT_1_TM=52.684 PRIMER_INTERNAL_1_TM=50.207 PRIMER_LEFT_1_GC_PERCENT=44.444 PRIMER_RIGHT_1_GC_PERCENT=26.923 PRIMER_INTERNAL_1_GC_PERCENT=25.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=9.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_INTERNAL_1_LIBRARY_MISHYB=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_LEFT_1_END_STABILITY=5.9000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=64 PRIMER_PAIR_2_PENALTY=8.000000 PRIMER_LEFT_2_PENALTY=0.000000 PRIMER_RIGHT_2_PENALTY=0.000000 PRIMER_INTERNAL_2_PENALTY=8.000000 PRIMER_LEFT_2_SEQUENCE=GCCCAAATCCCATTATTC PRIMER_RIGHT_2_SEQUENCE=TTATGTATAGGTTATTCATACCTCAT PRIMER_INTERNAL_2_SEQUENCE=ATAATGCATTTCAAGGTAAA PRIMER_LEFT_2=38,18 PRIMER_RIGHT_2=102,26 PRIMER_INTERNAL_2=56,20 PRIMER_LEFT_2_TM=55.228 PRIMER_RIGHT_2_TM=52.684 PRIMER_INTERNAL_2_TM=50.207 PRIMER_LEFT_2_GC_PERCENT=44.444 PRIMER_RIGHT_2_GC_PERCENT=26.923 PRIMER_INTERNAL_2_GC_PERCENT=25.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=9.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_INTERNAL_2_LIBRARY_MISHYB=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_LEFT_2_END_STABILITY=5.9000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=65 PRIMER_PAIR_3_PENALTY=8.000000 PRIMER_LEFT_3_PENALTY=0.000000 PRIMER_RIGHT_3_PENALTY=0.000000 PRIMER_INTERNAL_3_PENALTY=8.000000 PRIMER_LEFT_3_SEQUENCE=GCCCAAATCCCATTATTC PRIMER_RIGHT_3_SEQUENCE=TTATGTATAGGTTATTCATACCTCATT PRIMER_INTERNAL_3_SEQUENCE=ATAATGCATTTCAAGGTAAA PRIMER_LEFT_3=38,18 PRIMER_RIGHT_3=102,27 PRIMER_INTERNAL_3=56,20 PRIMER_LEFT_3_TM=55.228 PRIMER_RIGHT_3_TM=54.045 PRIMER_INTERNAL_3_TM=50.207 PRIMER_LEFT_3_GC_PERCENT=44.444 PRIMER_RIGHT_3_GC_PERCENT=25.926 PRIMER_INTERNAL_3_GC_PERCENT=25.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=9.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_INTERNAL_3_LIBRARY_MISHYB=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_LEFT_3_END_STABILITY=5.9000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=65 PRIMER_PAIR_4_PENALTY=8.000000 PRIMER_LEFT_4_PENALTY=0.000000 PRIMER_RIGHT_4_PENALTY=0.000000 PRIMER_INTERNAL_4_PENALTY=8.000000 PRIMER_LEFT_4_SEQUENCE=GCCCAAATCCCATTATTC PRIMER_RIGHT_4_SEQUENCE=ATTATGTATAGGTTATTCATACCTCA PRIMER_INTERNAL_4_SEQUENCE=ATAATGCATTTCAAGGTAAA PRIMER_LEFT_4=38,18 PRIMER_RIGHT_4=103,26 PRIMER_INTERNAL_4=56,20 PRIMER_LEFT_4_TM=55.228 PRIMER_RIGHT_4_TM=52.684 PRIMER_INTERNAL_4_TM=50.207 PRIMER_LEFT_4_GC_PERCENT=44.444 PRIMER_RIGHT_4_GC_PERCENT=26.923 PRIMER_INTERNAL_4_GC_PERCENT=25.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=9.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_INTERNAL_4_LIBRARY_MISHYB=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus PRIMER_LEFT_4_END_STABILITY=5.9000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=66 = SEQUENCE_ID=end_stability PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_INTERNAL_MISHYB_LIBRARY= PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PAIR_WT_PR_PENALTY=1 PRIMER_PAIR_WT_IO_PENALTY=0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0 PRIMER_WT_END_STABILITY=1 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCG PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 PRIMER_LEFT_EXPLAIN=considered 1130, low tm 2, high tm 643, ok 485 PRIMER_RIGHT_EXPLAIN=considered 1130, high tm 722, ok 408 PRIMER_PAIR_EXPLAIN=considered 1048, unacceptable product size 646, tm diff too large 287, ok 115 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=12.500000 PRIMER_LEFT_0_PENALTY=6.100000 PRIMER_RIGHT_0_PENALTY=6.400000 PRIMER_LEFT_0_SEQUENCE=ACCCACGAGTGAAGACTC PRIMER_RIGHT_0_SEQUENCE=TGGTGAGTTGAGGGAGAG PRIMER_LEFT_0=45,18 PRIMER_RIGHT_0=101,18 PRIMER_LEFT_0_TM=53.119 PRIMER_RIGHT_0_TM=54.310 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_RIGHT_0_GC_PERCENT=55.556 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=1.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.1000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=57 PRIMER_PAIR_1_PENALTY=12.500000 PRIMER_LEFT_1_PENALTY=6.100000 PRIMER_RIGHT_1_PENALTY=6.400000 PRIMER_LEFT_1_SEQUENCE=ACCCACGAGTGAAGACTC PRIMER_RIGHT_1_SEQUENCE=ATGGTGAGTTGAGGGAGAG PRIMER_LEFT_1=45,18 PRIMER_RIGHT_1=102,19 PRIMER_LEFT_1_TM=53.119 PRIMER_RIGHT_1_TM=54.979 PRIMER_LEFT_1_GC_PERCENT=55.556 PRIMER_RIGHT_1_GC_PERCENT=52.632 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.1000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=58 PRIMER_PAIR_2_PENALTY=12.500000 PRIMER_LEFT_2_PENALTY=6.100000 PRIMER_RIGHT_2_PENALTY=6.400000 PRIMER_LEFT_2_SEQUENCE=CACCCACGAGTGAAGACT PRIMER_RIGHT_2_SEQUENCE=TGGTGAGTTGAGGGAGAG PRIMER_LEFT_2=44,18 PRIMER_RIGHT_2=101,18 PRIMER_LEFT_2_TM=54.245 PRIMER_RIGHT_2_TM=54.310 PRIMER_LEFT_2_GC_PERCENT=55.556 PRIMER_RIGHT_2_GC_PERCENT=55.556 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.1000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=58 PRIMER_PAIR_3_PENALTY=12.500000 PRIMER_LEFT_3_PENALTY=6.100000 PRIMER_RIGHT_3_PENALTY=6.400000 PRIMER_LEFT_3_SEQUENCE=CACCCACGAGTGAAGACTC PRIMER_RIGHT_3_SEQUENCE=TGGTGAGTTGAGGGAGAG PRIMER_LEFT_3=44,19 PRIMER_RIGHT_3=101,18 PRIMER_LEFT_3_TM=56.607 PRIMER_RIGHT_3_TM=54.310 PRIMER_LEFT_3_GC_PERCENT=57.895 PRIMER_RIGHT_3_GC_PERCENT=55.556 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=1.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.1000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=58 PRIMER_PAIR_4_PENALTY=12.500000 PRIMER_LEFT_4_PENALTY=6.100000 PRIMER_RIGHT_4_PENALTY=6.400000 PRIMER_LEFT_4_SEQUENCE=CACCCACGAGTGAAGACT PRIMER_RIGHT_4_SEQUENCE=ATGGTGAGTTGAGGGAGAG PRIMER_LEFT_4=44,18 PRIMER_RIGHT_4=102,19 PRIMER_LEFT_4_TM=54.245 PRIMER_RIGHT_4_TM=54.979 PRIMER_LEFT_4_GC_PERCENT=55.556 PRIMER_RIGHT_4_GC_PERCENT=52.632 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.1000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=59 = SEQUENCE_ID=position_penalty SEQUENCE_TARGET=298,45 PRIMER_OUTSIDE_PENALTY=20.0 PRIMER_INSIDE_PENALTY=1000.0 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=36-2000 PRIMER_WT_POS_PENALTY=1 PRIMER_WT_END_STABILITY=0 SEQUENCE_TEMPLATE=TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTCCCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACTTTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCTCTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCAGGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTCCCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAA PRIMER_LEFT_EXPLAIN=considered 3260, overlap target 45, low tm 300, high tm 587, ok 2328 PRIMER_RIGHT_EXPLAIN=considered 1064, overlap target 289, low tm 64, high tm 149, ok 562 PRIMER_PAIR_EXPLAIN=considered 16, tm diff too large 9, ok 7 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.000000 PRIMER_LEFT_0_PENALTY=0.000000 PRIMER_RIGHT_0_PENALTY=0.000000 PRIMER_LEFT_0_SEQUENCE=AGCCCTGTGGAAGATTAGCGG PRIMER_RIGHT_0_SEQUENCE=CAAGGGGAAAGCTCACCCCTCTT PRIMER_LEFT_0=277,21 PRIMER_RIGHT_0=365,23 PRIMER_LEFT_0_TM=64.839 PRIMER_RIGHT_0_TM=67.828 PRIMER_LEFT_0_GC_PERCENT=57.143 PRIMER_RIGHT_0_GC_PERCENT=56.522 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=11.4000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=89 PRIMER_PAIR_1_PENALTY=0.000000 PRIMER_LEFT_1_PENALTY=0.000000 PRIMER_RIGHT_1_PENALTY=0.000000 PRIMER_LEFT_1_SEQUENCE=CAGCCCTGTGGAAGATTAGCGG PRIMER_RIGHT_1_SEQUENCE=CAAGGGGAAAGCTCACCCCTCTT PRIMER_LEFT_1=276,22 PRIMER_RIGHT_1=365,23 PRIMER_LEFT_1_TM=67.212 PRIMER_RIGHT_1_TM=67.828 PRIMER_LEFT_1_GC_PERCENT=59.091 PRIMER_RIGHT_1_GC_PERCENT=56.522 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=11.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=90 PRIMER_PAIR_2_PENALTY=0.000000 PRIMER_LEFT_2_PENALTY=0.000000 PRIMER_RIGHT_2_PENALTY=0.000000 PRIMER_LEFT_2_SEQUENCE=CCAGCCCTGTGGAAGATTAGCGG PRIMER_RIGHT_2_SEQUENCE=CAAGGGGAAAGCTCACCCCTCTT PRIMER_LEFT_2=275,23 PRIMER_RIGHT_2=365,23 PRIMER_LEFT_2_TM=69.870 PRIMER_RIGHT_2_TM=67.828 PRIMER_LEFT_2_GC_PERCENT=60.870 PRIMER_RIGHT_2_GC_PERCENT=56.522 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=10.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=0.000000 PRIMER_LEFT_2_END_STABILITY=11.4000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=91 PRIMER_PAIR_3_PENALTY=0.000000 PRIMER_LEFT_3_PENALTY=0.000000 PRIMER_RIGHT_3_PENALTY=0.000000 PRIMER_LEFT_3_SEQUENCE=GCCCTGTGGAAGATTAGCGG PRIMER_RIGHT_3_SEQUENCE=AAGGGGAAAGCTCACCCCTCTT PRIMER_LEFT_3=278,20 PRIMER_RIGHT_3=364,22 PRIMER_LEFT_3_TM=64.136 PRIMER_RIGHT_3_TM=65.600 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=54.545 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=10.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.000000 PRIMER_LEFT_3_END_STABILITY=11.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=87 PRIMER_PAIR_4_PENALTY=0.000000 PRIMER_LEFT_4_PENALTY=0.000000 PRIMER_RIGHT_4_PENALTY=0.000000 PRIMER_LEFT_4_SEQUENCE=AGCCCTGTGGAAGATTAGCGG PRIMER_RIGHT_4_SEQUENCE=AAGGGGAAAGCTCACCCCTCTT PRIMER_LEFT_4=277,21 PRIMER_RIGHT_4=364,22 PRIMER_LEFT_4_TM=64.839 PRIMER_RIGHT_4_TM=65.600 PRIMER_LEFT_4_GC_PERCENT=57.143 PRIMER_RIGHT_4_GC_PERCENT=54.545 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=10.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=0.000000 PRIMER_LEFT_4_END_STABILITY=11.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=88 = SEQUENCE_ID=sequence_quality PRIMER_WT_POS_PENALTY=0 PRIMER_WT_SEQ_QUAL=1 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATCGTACTACGTAGCTACTAGTCATCTAGCTAGCTAGTCGATGC PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_QUALITY_RANGE_MAX=70 SEQUENCE_QUALITY=10 20 30 40 50 50 50 50 50 50 50 60 50 50 60 50 50 50 50 50 50 55 50 50 55 50 60 50 50 50 50 60 60 60 60 60 60 60 20 20 20 30 30 30 50 50 30 53 30 50 60 60 60 60 60 60 60 50 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 70 50 50 50 50 50 50 50 50 50 50 50 60 60 60 60 60 60 60 60 60 60 50 50 50 50 50 20 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0 PRIMER_LEFT_EXPLAIN=considered 720, low tm 219, ok 501 PRIMER_RIGHT_EXPLAIN=considered 720, low tm 282, ok 438 PRIMER_PAIR_EXPLAIN=considered 54742, unacceptable product size 32903, tm diff too large 13218, high any compl 329, ok 8292 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=40.000000 PRIMER_LEFT_0_PENALTY=20.000000 PRIMER_RIGHT_0_PENALTY=20.000000 PRIMER_LEFT_0_SEQUENCE=TGATCATCGTAGCTAGCTAG PRIMER_RIGHT_0_SEQUENCE=ATGACTAGTAGCTACGTAGTACG PRIMER_LEFT_0=52,20 PRIMER_RIGHT_0=101,23 PRIMER_LEFT_0_TM=50.931 PRIMER_RIGHT_0_TM=50.484 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=10.00 PRIMER_RIGHT_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=8.00 PRIMER_RIGHT_0_SELF_END=8.00 PRIMER_LEFT_0_MIN_SEQ_QUALITY=50 PRIMER_RIGHT_0_MIN_SEQ_QUALITY=50 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=7.1000 PRIMER_PAIR_0_COMPL_ANY=10.00 PRIMER_PAIR_0_COMPL_END=7.00 PRIMER_PAIR_0_PRODUCT_SIZE=50 PRIMER_PAIR_1_PENALTY=40.000000 PRIMER_LEFT_1_PENALTY=20.000000 PRIMER_RIGHT_1_PENALTY=20.000000 PRIMER_LEFT_1_SEQUENCE=TGATCATCGTAGCTAGCTAG PRIMER_RIGHT_1_SEQUENCE=ATGACTAGTAGCTACGTAGTACGA PRIMER_LEFT_1=52,20 PRIMER_RIGHT_1=101,24 PRIMER_LEFT_1_TM=50.931 PRIMER_RIGHT_1_TM=52.419 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=10.00 PRIMER_RIGHT_1_SELF_ANY=12.00 PRIMER_LEFT_1_SELF_END=8.00 PRIMER_RIGHT_1_SELF_END=10.00 PRIMER_LEFT_1_MIN_SEQ_QUALITY=50 PRIMER_RIGHT_1_MIN_SEQ_QUALITY=50 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=7.4000 PRIMER_PAIR_1_COMPL_ANY=10.00 PRIMER_PAIR_1_COMPL_END=7.00 PRIMER_PAIR_1_PRODUCT_SIZE=50 PRIMER_PAIR_2_PENALTY=40.000000 PRIMER_LEFT_2_PENALTY=20.000000 PRIMER_RIGHT_2_PENALTY=20.000000 PRIMER_LEFT_2_SEQUENCE=TGATCATCGTAGCTAGCTAG PRIMER_RIGHT_2_SEQUENCE=ATGACTAGTAGCTACGTAGTACGAT PRIMER_LEFT_2=52,20 PRIMER_RIGHT_2=101,25 PRIMER_LEFT_2_TM=50.931 PRIMER_RIGHT_2_TM=53.013 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=10.00 PRIMER_RIGHT_2_SELF_ANY=12.00 PRIMER_LEFT_2_SELF_END=8.00 PRIMER_RIGHT_2_SELF_END=10.00 PRIMER_LEFT_2_MIN_SEQ_QUALITY=50 PRIMER_RIGHT_2_MIN_SEQ_QUALITY=50 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=0.000000 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=8.0000 PRIMER_PAIR_2_COMPL_ANY=10.00 PRIMER_PAIR_2_COMPL_END=8.00 PRIMER_PAIR_2_PRODUCT_SIZE=50 PRIMER_PAIR_3_PENALTY=40.000000 PRIMER_LEFT_3_PENALTY=20.000000 PRIMER_RIGHT_3_PENALTY=20.000000 PRIMER_LEFT_3_SEQUENCE=TGATCATCGTAGCTAGCTAGT PRIMER_RIGHT_3_SEQUENCE=ATGACTAGTAGCTACGTAGTACG PRIMER_LEFT_3=52,21 PRIMER_RIGHT_3=101,23 PRIMER_LEFT_3_TM=52.135 PRIMER_RIGHT_3_TM=50.484 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=10.00 PRIMER_RIGHT_3_SELF_ANY=12.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=8.00 PRIMER_LEFT_3_MIN_SEQ_QUALITY=50 PRIMER_RIGHT_3_MIN_SEQ_QUALITY=50 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.000000 PRIMER_LEFT_3_END_STABILITY=5.4000 PRIMER_RIGHT_3_END_STABILITY=7.1000 PRIMER_PAIR_3_COMPL_ANY=11.00 PRIMER_PAIR_3_COMPL_END=8.00 PRIMER_PAIR_3_PRODUCT_SIZE=50 PRIMER_PAIR_4_PENALTY=40.000000 PRIMER_LEFT_4_PENALTY=20.000000 PRIMER_RIGHT_4_PENALTY=20.000000 PRIMER_LEFT_4_SEQUENCE=TGATCATCGTAGCTAGCTAGT PRIMER_RIGHT_4_SEQUENCE=ATGACTAGTAGCTACGTAGTACGA PRIMER_LEFT_4=52,21 PRIMER_RIGHT_4=101,24 PRIMER_LEFT_4_TM=52.135 PRIMER_RIGHT_4_TM=52.419 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=10.00 PRIMER_RIGHT_4_SELF_ANY=12.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=10.00 PRIMER_LEFT_4_MIN_SEQ_QUALITY=50 PRIMER_RIGHT_4_MIN_SEQ_QUALITY=50 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=0.000000 PRIMER_LEFT_4_END_STABILITY=5.4000 PRIMER_RIGHT_4_END_STABILITY=7.4000 PRIMER_PAIR_4_COMPL_ANY=11.00 PRIMER_PAIR_4_COMPL_END=8.00 PRIMER_PAIR_4_PRODUCT_SIZE=50 = SEQUENCE_ID=sequence_quality_io PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_SEQ_QUAL=1 PRIMER_PAIR_MAX_DIFF_TM=20 PRIMER_MIN_TM=20 PRIMER_MAX_TM=100 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 PRIMER_INTERNAL_MAX_SELF_ANY=20 PRIMER_INTERNAL_MAX_SELF_END=20 PRIMER_INTERNAL_MIN_TM=20 PRIMER_INTERNAL_MAX_TM=100 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=20 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=20 PRIMER_PAIR_MAX_COMPL_ANY=20 PRIMER_PAIR_MAX_COMPL_END=20 PRIMER_INTERNAL_WT_END_QUAL=2 PRIMER_PAIR_WT_PR_PENALTY=0 PRIMER_PAIR_WT_IO_PENALTY=1 PRIMER_WT_SEQ_QUAL=0 PRIMER_WT_END_QUAL=0 SEQUENCE_TEMPLATE=ATCGTAGCTGATCGTAGCTGATCGTAGCTAGTCAGTCTACTACTGATCTAGCTGATCATCGTAGCTAGCTAGTCATCATC SEQUENCE_QUALITY=10 20 30 40 50 50 50 50 50 50 50 60 50 50 60 50 50 50 50 50 50 55 50 50 55 50 60 50 50 50 50 60 60 60 60 60 60 60 20 20 20 30 30 30 50 50 30 53 30 50 60 60 60 60 60 60 60 50 50 50 50 50 50 50 50 50 70 70 70 70 70 70 70 70 70 70 70 70 70 70 PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0 PRIMER_LEFT_EXPLAIN=considered 310, ok 310 PRIMER_RIGHT_EXPLAIN=considered 310, ok 310 PRIMER_INTERNAL_EXPLAIN=considered 585, ok 585 PRIMER_PAIR_EXPLAIN=considered 96100, unacceptable product size 46500, tm diff too large 938, no internal oligo 30861, ok 17801 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=20.000000 PRIMER_LEFT_0_PENALTY=0.000000 PRIMER_RIGHT_0_PENALTY=0.000000 PRIMER_INTERNAL_0_PENALTY=20.000000 PRIMER_LEFT_0_SEQUENCE=CGTAGCTGATCGTAGCTG PRIMER_RIGHT_0_SEQUENCE=ATCAGCTAGATCAGTAGT PRIMER_INTERNAL_0_SEQUENCE=ATCGTAGCTAGTCAGTCT PRIMER_LEFT_0=2,18 PRIMER_RIGHT_0=55,18 PRIMER_INTERNAL_0=20,18 PRIMER_LEFT_0_TM=52.936 PRIMER_RIGHT_0_TM=40.383 PRIMER_INTERNAL_0_TM=43.690 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_RIGHT_0_GC_PERCENT=38.889 PRIMER_INTERNAL_0_GC_PERCENT=44.444 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_MIN_SEQ_QUALITY=30 PRIMER_RIGHT_0_MIN_SEQ_QUALITY=20 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=5.1000 PRIMER_INTERNAL_0_MIN_SEQ_QUALITY=50 PRIMER_PAIR_0_COMPL_ANY=10.00 PRIMER_PAIR_0_COMPL_END=9.00 PRIMER_PAIR_0_PRODUCT_SIZE=54 PRIMER_PAIR_1_PENALTY=20.000000 PRIMER_LEFT_1_PENALTY=0.000000 PRIMER_RIGHT_1_PENALTY=0.000000 PRIMER_INTERNAL_1_PENALTY=20.000000 PRIMER_LEFT_1_SEQUENCE=CGTAGCTGATCGTAGCTG PRIMER_RIGHT_1_SEQUENCE=GATCAGCTAGATCAGTAG PRIMER_INTERNAL_1_SEQUENCE=ATCGTAGCTAGTCAGTCT PRIMER_LEFT_1=2,18 PRIMER_RIGHT_1=56,18 PRIMER_INTERNAL_1=20,18 PRIMER_LEFT_1_TM=52.936 PRIMER_RIGHT_1_TM=41.431 PRIMER_INTERNAL_1_TM=43.690 PRIMER_LEFT_1_GC_PERCENT=55.556 PRIMER_RIGHT_1_GC_PERCENT=44.444 PRIMER_INTERNAL_1_GC_PERCENT=44.444 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=3.00 PRIMER_LEFT_1_MIN_SEQ_QUALITY=30 PRIMER_RIGHT_1_MIN_SEQ_QUALITY=20 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=5.4000 PRIMER_INTERNAL_1_MIN_SEQ_QUALITY=50 PRIMER_PAIR_1_COMPL_ANY=10.00 PRIMER_PAIR_1_COMPL_END=9.00 PRIMER_PAIR_1_PRODUCT_SIZE=55 PRIMER_PAIR_2_PENALTY=20.000000 PRIMER_LEFT_2_PENALTY=0.000000 PRIMER_RIGHT_2_PENALTY=0.000000 PRIMER_INTERNAL_2_PENALTY=20.000000 PRIMER_LEFT_2_SEQUENCE=CGTAGCTGATCGTAGCTG PRIMER_RIGHT_2_SEQUENCE=GATCAGCTAGATCAGTAGT PRIMER_INTERNAL_2_SEQUENCE=ATCGTAGCTAGTCAGTCT PRIMER_LEFT_2=2,18 PRIMER_RIGHT_2=56,19 PRIMER_INTERNAL_2=20,18 PRIMER_LEFT_2_TM=52.936 PRIMER_RIGHT_2_TM=43.262 PRIMER_INTERNAL_2_TM=43.690 PRIMER_LEFT_2_GC_PERCENT=55.556 PRIMER_RIGHT_2_GC_PERCENT=42.105 PRIMER_INTERNAL_2_GC_PERCENT=44.444 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=3.00 PRIMER_LEFT_2_MIN_SEQ_QUALITY=30 PRIMER_RIGHT_2_MIN_SEQ_QUALITY=20 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=0.000000 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=5.1000 PRIMER_INTERNAL_2_MIN_SEQ_QUALITY=50 PRIMER_PAIR_2_COMPL_ANY=10.00 PRIMER_PAIR_2_COMPL_END=9.00 PRIMER_PAIR_2_PRODUCT_SIZE=55 PRIMER_PAIR_3_PENALTY=20.000000 PRIMER_LEFT_3_PENALTY=0.000000 PRIMER_RIGHT_3_PENALTY=0.000000 PRIMER_INTERNAL_3_PENALTY=20.000000 PRIMER_LEFT_3_SEQUENCE=CGTAGCTGATCGTAGCTG PRIMER_RIGHT_3_SEQUENCE=TGATCAGCTAGATCAGTA PRIMER_INTERNAL_3_SEQUENCE=ATCGTAGCTAGTCAGTCT PRIMER_LEFT_3=2,18 PRIMER_RIGHT_3=57,18 PRIMER_INTERNAL_3=20,18 PRIMER_LEFT_3_TM=52.936 PRIMER_RIGHT_3_TM=43.313 PRIMER_INTERNAL_3_TM=43.690 PRIMER_LEFT_3_GC_PERCENT=55.556 PRIMER_RIGHT_3_GC_PERCENT=38.889 PRIMER_INTERNAL_3_GC_PERCENT=44.444 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=10.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=3.00 PRIMER_LEFT_3_MIN_SEQ_QUALITY=30 PRIMER_RIGHT_3_MIN_SEQ_QUALITY=20 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.000000 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=5.7000 PRIMER_INTERNAL_3_MIN_SEQ_QUALITY=50 PRIMER_PAIR_3_COMPL_ANY=10.00 PRIMER_PAIR_3_COMPL_END=10.00 PRIMER_PAIR_3_PRODUCT_SIZE=56 PRIMER_PAIR_4_PENALTY=20.000000 PRIMER_LEFT_4_PENALTY=0.000000 PRIMER_RIGHT_4_PENALTY=0.000000 PRIMER_INTERNAL_4_PENALTY=20.000000 PRIMER_LEFT_4_SEQUENCE=CGTAGCTGATCGTAGCTG PRIMER_RIGHT_4_SEQUENCE=TGATCAGCTAGATCAGTAG PRIMER_INTERNAL_4_SEQUENCE=ATCGTAGCTAGTCAGTCT PRIMER_LEFT_4=2,18 PRIMER_RIGHT_4=57,19 PRIMER_INTERNAL_4=20,18 PRIMER_LEFT_4_TM=52.936 PRIMER_RIGHT_4_TM=45.394 PRIMER_INTERNAL_4_TM=43.690 PRIMER_LEFT_4_GC_PERCENT=55.556 PRIMER_RIGHT_4_GC_PERCENT=42.105 PRIMER_INTERNAL_4_GC_PERCENT=44.444 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=10.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_MIN_SEQ_QUALITY=30 PRIMER_RIGHT_4_MIN_SEQ_QUALITY=20 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=0.000000 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=5.4000 PRIMER_INTERNAL_4_MIN_SEQ_QUALITY=50 PRIMER_PAIR_4_COMPL_ANY=10.00 PRIMER_PAIR_4_COMPL_END=9.00 PRIMER_PAIR_4_PRODUCT_SIZE=56 = SEQUENCE_ID=io_gc_content PRIMER_WT_SEQ_QUAL=0 PRIMER_INTERNAL_WT_SEQ_QUAL=0 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_GC=40 PRIMER_INTERNAL_OPT_GC_PERCENT=50 PRIMER_INTERNAL_MAX_GC=60 PRIMER_INTERNAL_WT_GC_PERCENT_GT=1 PRIMER_INTERNAL_WT_GC_PERCENT_LT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PAIR_WT_IO_PENALTY=1 PRIMER_PAIR_WT_PR_PENALTY=0 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0 PRIMER_LEFT_EXPLAIN=considered 1290, ok 1290 PRIMER_RIGHT_EXPLAIN=considered 1290, ok 1290 PRIMER_INTERNAL_EXPLAIN=considered 1565, GC content failed 1015, ok 550 PRIMER_PAIR_EXPLAIN=considered 522, tm diff too large 2, no internal oligo 463, ok 57 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.000000 PRIMER_LEFT_0_PENALTY=0.000000 PRIMER_RIGHT_0_PENALTY=0.000000 PRIMER_INTERNAL_0_PENALTY=0.000000 PRIMER_LEFT_0_SEQUENCE=TCGGGCCTCTCCCTCAAC PRIMER_RIGHT_0_SEQUENCE=CCGGCGCAGGAGCCACCT PRIMER_INTERNAL_0_SEQUENCE=TCACCATGATGACCGTCA PRIMER_LEFT_0=78,18 PRIMER_RIGHT_0=177,18 PRIMER_INTERNAL_0=96,18 PRIMER_LEFT_0_TM=63.720 PRIMER_RIGHT_0_TM=71.517 PRIMER_INTERNAL_0_TM=57.693 PRIMER_LEFT_0_GC_PERCENT=66.667 PRIMER_RIGHT_0_GC_PERCENT=77.778 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_INTERNAL_0_SELF_END=7.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=100 PRIMER_PAIR_1_PENALTY=0.000000 PRIMER_LEFT_1_PENALTY=0.000000 PRIMER_RIGHT_1_PENALTY=0.000000 PRIMER_INTERNAL_1_PENALTY=0.000000 PRIMER_LEFT_1_SEQUENCE=CTCGGGCCTCTCCCTCAA PRIMER_RIGHT_1_SEQUENCE=CCGGCGCAGGAGCCACCT PRIMER_INTERNAL_1_SEQUENCE=TCACCATGATGACCGTCA PRIMER_LEFT_1=77,18 PRIMER_RIGHT_1=177,18 PRIMER_INTERNAL_1=96,18 PRIMER_LEFT_1_TM=63.831 PRIMER_RIGHT_1_TM=71.517 PRIMER_INTERNAL_1_TM=57.693 PRIMER_LEFT_1_GC_PERCENT=66.667 PRIMER_RIGHT_1_GC_PERCENT=77.778 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_INTERNAL_1_SELF_END=7.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=101 PRIMER_PAIR_2_PENALTY=0.000000 PRIMER_LEFT_2_PENALTY=0.000000 PRIMER_RIGHT_2_PENALTY=0.000000 PRIMER_INTERNAL_2_PENALTY=0.000000 PRIMER_LEFT_2_SEQUENCE=CTCGGGCCTCTCCCTCAAC PRIMER_RIGHT_2_SEQUENCE=CCGGCGCAGGAGCCACCT PRIMER_INTERNAL_2_SEQUENCE=TCACCATGATGACCGTCA PRIMER_LEFT_2=77,19 PRIMER_RIGHT_2=177,18 PRIMER_INTERNAL_2=96,18 PRIMER_LEFT_2_TM=64.527 PRIMER_RIGHT_2_TM=71.517 PRIMER_INTERNAL_2_TM=57.693 PRIMER_LEFT_2_GC_PERCENT=68.421 PRIMER_RIGHT_2_GC_PERCENT=77.778 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_INTERNAL_2_SELF_END=7.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=0.000000 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=101 PRIMER_PAIR_3_PENALTY=0.000000 PRIMER_LEFT_3_PENALTY=0.000000 PRIMER_RIGHT_3_PENALTY=0.000000 PRIMER_INTERNAL_3_PENALTY=0.000000 PRIMER_LEFT_3_SEQUENCE=CCTCGGGCCTCTCCCTCA PRIMER_RIGHT_3_SEQUENCE=CCGGCGCAGGAGCCACCT PRIMER_INTERNAL_3_SEQUENCE=TCACCATGATGACCGTCA PRIMER_LEFT_3=76,18 PRIMER_RIGHT_3=177,18 PRIMER_INTERNAL_3=96,18 PRIMER_LEFT_3_TM=66.184 PRIMER_RIGHT_3_TM=71.517 PRIMER_INTERNAL_3_TM=57.693 PRIMER_LEFT_3_GC_PERCENT=72.222 PRIMER_RIGHT_3_GC_PERCENT=77.778 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_INTERNAL_3_SELF_END=7.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.000000 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=102 PRIMER_PAIR_4_PENALTY=0.000000 PRIMER_LEFT_4_PENALTY=0.000000 PRIMER_RIGHT_4_PENALTY=0.000000 PRIMER_INTERNAL_4_PENALTY=0.000000 PRIMER_LEFT_4_SEQUENCE=CCTCGGGCCTCTCCCTCAA PRIMER_RIGHT_4_SEQUENCE=CCGGCGCAGGAGCCACCT PRIMER_INTERNAL_4_SEQUENCE=TCACCATGATGACCGTCA PRIMER_LEFT_4=76,19 PRIMER_RIGHT_4=177,18 PRIMER_INTERNAL_4=96,18 PRIMER_LEFT_4_TM=67.190 PRIMER_RIGHT_4_TM=71.517 PRIMER_INTERNAL_4_TM=57.693 PRIMER_LEFT_4_GC_PERCENT=68.421 PRIMER_RIGHT_4_GC_PERCENT=77.778 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_INTERNAL_4_SELF_END=7.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=0.000000 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=102 = SEQUENCE_ID=primer_gc_content PRIMER_WT_SEQ_QUAL=0 PRIMER_INTERNAL_WT_SEQ_QUAL=0 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 PRIMER_MIN_GC=40 PRIMER_OPT_GC_PERCENT=50 PRIMER_MAX_GC=60 PRIMER_WT_GC_PERCENT_GT=1 PRIMER_WT_GC_PERCENT_LT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PAIR_WT_PR_PENALTY=1 PRIMER_WT_TM_GT=0 PRIMER_WT_TM_LT=0 PRIMER_WT_SIZE_LT=0 PRIMER_WT_SIZE_GT=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_WT_TM_GT=0 PRIMER_INTERNAL_WT_TM_LT=0 PRIMER_INTERNAL_WT_SIZE_LT=0 PRIMER_INTERNAL_WT_SIZE_GT=0 PRIMER_INTERNAL_WT_SELF_ANY=0 PRIMER_INTERNAL_WT_SELF_END=0 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0 PRIMER_LEFT_EXPLAIN=considered 1290, GC content failed 740, ok 550 PRIMER_RIGHT_EXPLAIN=considered 1290, GC content failed 932, ok 358 PRIMER_INTERNAL_EXPLAIN=considered 1565, GC content failed 1015, ok 550 PRIMER_PAIR_EXPLAIN=considered 50, unacceptable product size 30, no internal oligo 15, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.000000 PRIMER_LEFT_0_PENALTY=0.000000 PRIMER_RIGHT_0_PENALTY=0.000000 PRIMER_INTERNAL_0_PENALTY=0.000000 PRIMER_LEFT_0_SEQUENCE=ACTTCACTTTCCGACCCT PRIMER_RIGHT_0_SEQUENCE=CTGACGGTCATCATGGTGAGTT PRIMER_INTERNAL_0_SEQUENCE=TTTCACCCACGAGTGAAGACTC PRIMER_LEFT_0=12,18 PRIMER_RIGHT_0=114,22 PRIMER_INTERNAL_0=41,22 PRIMER_LEFT_0_TM=54.516 PRIMER_RIGHT_0_TM=62.314 PRIMER_INTERNAL_0_TM=61.614 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_INTERNAL_0_SELF_END=4.00 PRIMER_LEFT_0_POSITION_PENALTY=0.000000 PRIMER_RIGHT_0_POSITION_PENALTY=0.000000 PRIMER_LEFT_0_END_STABILITY=9.1000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=103 PRIMER_PAIR_1_PENALTY=0.000000 PRIMER_LEFT_1_PENALTY=0.000000 PRIMER_RIGHT_1_PENALTY=0.000000 PRIMER_INTERNAL_1_PENALTY=0.000000 PRIMER_LEFT_1_SEQUENCE=TGACTTCACTTTCCGACC PRIMER_RIGHT_1_SEQUENCE=CTGACGGTCATCATGGTGAGTT PRIMER_INTERNAL_1_SEQUENCE=TTTCACCCACGAGTGAAGACTC PRIMER_LEFT_1=10,18 PRIMER_RIGHT_1=114,22 PRIMER_INTERNAL_1=41,22 PRIMER_LEFT_1_TM=54.341 PRIMER_RIGHT_1_TM=62.314 PRIMER_INTERNAL_1_TM=61.614 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_INTERNAL_1_SELF_END=4.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=105 PRIMER_PAIR_2_PENALTY=0.000000 PRIMER_LEFT_2_PENALTY=0.000000 PRIMER_RIGHT_2_PENALTY=0.000000 PRIMER_INTERNAL_2_PENALTY=0.000000 PRIMER_LEFT_2_SEQUENCE=TGACTTCACTTTCCGACCCT PRIMER_RIGHT_2_SEQUENCE=CTGACGGTCATCATGGTGAGTT PRIMER_INTERNAL_2_SEQUENCE=TTTCACCCACGAGTGAAGACTC PRIMER_LEFT_2=10,20 PRIMER_RIGHT_2=114,22 PRIMER_INTERNAL_2=41,22 PRIMER_LEFT_2_TM=59.697 PRIMER_RIGHT_2_TM=62.314 PRIMER_INTERNAL_2_TM=61.614 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_INTERNAL_2_SELF_END=4.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=0.000000 PRIMER_LEFT_2_END_STABILITY=9.1000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=105 PRIMER_PAIR_3_PENALTY=0.000000 PRIMER_LEFT_3_PENALTY=0.000000 PRIMER_RIGHT_3_PENALTY=0.000000 PRIMER_INTERNAL_3_PENALTY=0.000000 PRIMER_LEFT_3_SEQUENCE=ATGACTTCACTTTCCGACCC PRIMER_RIGHT_3_SEQUENCE=CTGACGGTCATCATGGTGAGTT PRIMER_INTERNAL_3_SEQUENCE=TTTCACCCACGAGTGAAGACTC PRIMER_LEFT_3=9,20 PRIMER_RIGHT_3=114,22 PRIMER_INTERNAL_3=41,22 PRIMER_LEFT_3_TM=58.992 PRIMER_RIGHT_3_TM=62.314 PRIMER_INTERNAL_3_TM=61.614 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_INTERNAL_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_INTERNAL_3_SELF_END=4.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.000000 PRIMER_LEFT_3_END_STABILITY=9.1000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=106 PRIMER_PAIR_4_PENALTY=0.000000 PRIMER_LEFT_4_PENALTY=0.000000 PRIMER_RIGHT_4_PENALTY=0.000000 PRIMER_INTERNAL_4_PENALTY=0.000000 PRIMER_LEFT_4_SEQUENCE=ATGACTTCACTTTCCGACCCTG PRIMER_RIGHT_4_SEQUENCE=CTGACGGTCATCATGGTGAGTT PRIMER_INTERNAL_4_SEQUENCE=TTTCACCCACGAGTGAAGACTC PRIMER_LEFT_4=9,22 PRIMER_RIGHT_4=114,22 PRIMER_INTERNAL_4=41,22 PRIMER_LEFT_4_TM=62.662 PRIMER_RIGHT_4_TM=62.314 PRIMER_INTERNAL_4_TM=61.614 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_INTERNAL_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_INTERNAL_4_SELF_END=4.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=0.000000 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=106 = primer3-2.3.6/test/primer_mispriming_input0000664000077300007730000000675312231156245017746 0ustar ioanaioanaSEQUENCE_ID=exon1 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG SEQUENCE_EXCLUDED_REGION=100,150 PRIMER_PRODUCT_SIZE_RANGE=200-250 PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 PRIMER_PAIR_MAX_DIFF_TM=3 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib = SEQUENCE_ID=exon2 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 = PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_ID=exon2 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 = SEQUENCE_ID=exon1_2 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG SEQUENCE_EXCLUDED_REGION=100,150 PRIMER_PRODUCT_SIZE_RANGE=200-250 PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 PRIMER_PAIR_MAX_DIFF_TM=3 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=16 = SEQUENCE_ID=exon2_2 PRIMER_NUM_RETURN=7 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 = PRIMER_NUM_RETURN=5 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_ID=exon2_3 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=10 = primer3-2.3.6/test/primer_must_overlap_point_output0000664000077300007730000007451212231156245021720 0ustar ioanaioanaSEQUENCE_ID=Medicago Lyk3 mRNA regular pick SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.100920 PRIMER_LEFT_0_PENALTY=0.025128 PRIMER_RIGHT_0_PENALTY=0.075792 PRIMER_LEFT_0_SEQUENCE=aaatttgcgtggaaaggttg PRIMER_RIGHT_0_SEQUENCE=ttctggtggcatgtatccaa PRIMER_LEFT_0=1362,20 PRIMER_RIGHT_0=1470,20 PRIMER_LEFT_0_TM=59.975 PRIMER_RIGHT_0_TM=59.924 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=109 PRIMER_PAIR_1_PENALTY=0.127762 PRIMER_LEFT_1_PENALTY=0.046801 PRIMER_RIGHT_1_PENALTY=0.080962 PRIMER_LEFT_1_SEQUENCE=tacaggcattatggtggcaa PRIMER_RIGHT_1_SEQUENCE=gaaattcggacgatgcttgt PRIMER_LEFT_1=906,20 PRIMER_RIGHT_1=1087,20 PRIMER_LEFT_1_TM=59.953 PRIMER_RIGHT_1_TM=60.081 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=10.0000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=182 PRIMER_PAIR_2_PENALTY=0.127762 PRIMER_LEFT_2_PENALTY=0.046801 PRIMER_RIGHT_2_PENALTY=0.080962 PRIMER_LEFT_2_SEQUENCE=ttacaggcattatggtggca PRIMER_RIGHT_2_SEQUENCE=gaaattcggacgatgcttgt PRIMER_LEFT_2=905,20 PRIMER_RIGHT_2=1087,20 PRIMER_LEFT_2_TM=59.953 PRIMER_RIGHT_2_TM=60.081 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=10.0000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=183 PRIMER_PAIR_3_PENALTY=0.146540 PRIMER_LEFT_3_PENALTY=0.070748 PRIMER_RIGHT_3_PENALTY=0.075792 PRIMER_LEFT_3_SEQUENCE=atccatcgcgacgtaaaatc PRIMER_RIGHT_3_SEQUENCE=ttctggtggcatgtatccaa PRIMER_LEFT_3=1321,20 PRIMER_RIGHT_3=1470,20 PRIMER_LEFT_3_TM=59.929 PRIMER_RIGHT_3_TM=59.924 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=150 PRIMER_PAIR_4_PENALTY=0.157878 PRIMER_LEFT_4_PENALTY=0.046801 PRIMER_RIGHT_4_PENALTY=0.111077 PRIMER_LEFT_4_SEQUENCE=tacaggcattatggtggcaa PRIMER_RIGHT_4_SEQUENCE=acagctccaaatccaccttg PRIMER_LEFT_4=906,20 PRIMER_RIGHT_4=1016,20 PRIMER_LEFT_4_TM=59.953 PRIMER_RIGHT_4_TM=60.111 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=111 = SEQUENCE_ID=Medicago Lyk3 mRNA creates out of sequence error big SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=646 680 838 1041 1133 1228 1382 1469 1608 1706 2091 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 PRIMER_ERROR=SEQUENCE_OVERLAP_JUNCTION_LIST beyond end of sequence = SEQUENCE_ID=Medicago Lyk3 mRNA creates out of sequence error small SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=0 646 680 838 1041 1133 1228 1382 1469 1608 1706 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 PRIMER_ERROR=Negative SEQUENCE_OVERLAP_JUNCTION_LIST length = SEQUENCE_ID=Medicago Lyk3 mRNA picks primers overlapping one intron SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=646 680 838 1041 1133 1228 1382 1469 1608 1706 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.186184 PRIMER_LEFT_0_PENALTY=0.110392 PRIMER_RIGHT_0_PENALTY=0.075792 PRIMER_LEFT_0_SEQUENCE=cgtggaaaggttgctgattt PRIMER_RIGHT_0_SEQUENCE=ttctggtggcatgtatccaa PRIMER_LEFT_0=1369,20 PRIMER_RIGHT_0=1470,20 PRIMER_LEFT_0_TM=60.110 PRIMER_RIGHT_0_TM=59.924 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=102 PRIMER_PAIR_1_PENALTY=0.200547 PRIMER_LEFT_1_PENALTY=0.110392 PRIMER_RIGHT_1_PENALTY=0.090155 PRIMER_LEFT_1_SEQUENCE=cgtggaaaggttgctgattt PRIMER_RIGHT_1_SEQUENCE=tccaccaattttcgaagacc PRIMER_LEFT_1=1369,20 PRIMER_RIGHT_1=1664,20 PRIMER_LEFT_1_TM=60.110 PRIMER_RIGHT_1_TM=59.910 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=296 PRIMER_PAIR_2_PENALTY=0.244982 PRIMER_LEFT_2_PENALTY=0.025128 PRIMER_RIGHT_2_PENALTY=0.219854 PRIMER_LEFT_2_SEQUENCE=aaatttgcgtggaaaggttg PRIMER_RIGHT_2_SEQUENCE=ttgagcatattctggtggca PRIMER_LEFT_2=1362,20 PRIMER_RIGHT_2=1479,20 PRIMER_LEFT_2_TM=59.975 PRIMER_RIGHT_2_TM=60.220 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=118 PRIMER_PAIR_3_PENALTY=0.290602 PRIMER_LEFT_3_PENALTY=0.070748 PRIMER_RIGHT_3_PENALTY=0.219854 PRIMER_LEFT_3_SEQUENCE=atccatcgcgacgtaaaatc PRIMER_RIGHT_3_SEQUENCE=ttgagcatattctggtggca PRIMER_LEFT_3=1321,20 PRIMER_RIGHT_3=1479,20 PRIMER_LEFT_3_TM=59.929 PRIMER_RIGHT_3_TM=60.220 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=159 PRIMER_PAIR_4_PENALTY=0.310009 PRIMER_LEFT_4_PENALTY=0.219854 PRIMER_RIGHT_4_PENALTY=0.090155 PRIMER_LEFT_4_SEQUENCE=tgccaccagaatatgctcaa PRIMER_RIGHT_4_SEQUENCE=tccaccaattttcgaagacc PRIMER_LEFT_4=1460,20 PRIMER_RIGHT_4=1664,20 PRIMER_LEFT_4_TM=60.220 PRIMER_RIGHT_4_TM=59.910 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=205 = SEQUENCE_ID=Medicago Lyk3 mRNA picks primers overlapping one intron with included region SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=646 680 838 1041 1133 1228 1382 1469 1608 1706 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 SEQUENCE_INCLUDED_REGION=600,1400 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.186184 PRIMER_LEFT_0_PENALTY=0.110392 PRIMER_RIGHT_0_PENALTY=0.075792 PRIMER_LEFT_0_SEQUENCE=cgtggaaaggttgctgattt PRIMER_RIGHT_0_SEQUENCE=ttctggtggcatgtatccaa PRIMER_LEFT_0=1369,20 PRIMER_RIGHT_0=1470,20 PRIMER_LEFT_0_TM=60.110 PRIMER_RIGHT_0_TM=59.924 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=102 PRIMER_PAIR_1_PENALTY=0.200547 PRIMER_LEFT_1_PENALTY=0.110392 PRIMER_RIGHT_1_PENALTY=0.090155 PRIMER_LEFT_1_SEQUENCE=cgtggaaaggttgctgattt PRIMER_RIGHT_1_SEQUENCE=tccaccaattttcgaagacc PRIMER_LEFT_1=1369,20 PRIMER_RIGHT_1=1664,20 PRIMER_LEFT_1_TM=60.110 PRIMER_RIGHT_1_TM=59.910 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=296 PRIMER_PAIR_2_PENALTY=0.244982 PRIMER_LEFT_2_PENALTY=0.025128 PRIMER_RIGHT_2_PENALTY=0.219854 PRIMER_LEFT_2_SEQUENCE=aaatttgcgtggaaaggttg PRIMER_RIGHT_2_SEQUENCE=ttgagcatattctggtggca PRIMER_LEFT_2=1362,20 PRIMER_RIGHT_2=1479,20 PRIMER_LEFT_2_TM=59.975 PRIMER_RIGHT_2_TM=60.220 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=118 PRIMER_PAIR_3_PENALTY=0.290602 PRIMER_LEFT_3_PENALTY=0.070748 PRIMER_RIGHT_3_PENALTY=0.219854 PRIMER_LEFT_3_SEQUENCE=atccatcgcgacgtaaaatc PRIMER_RIGHT_3_SEQUENCE=ttgagcatattctggtggca PRIMER_LEFT_3=1321,20 PRIMER_RIGHT_3=1479,20 PRIMER_LEFT_3_TM=59.929 PRIMER_RIGHT_3_TM=60.220 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=159 PRIMER_PAIR_4_PENALTY=0.310009 PRIMER_LEFT_4_PENALTY=0.219854 PRIMER_RIGHT_4_PENALTY=0.090155 PRIMER_LEFT_4_SEQUENCE=tgccaccagaatatgctcaa PRIMER_RIGHT_4_SEQUENCE=tccaccaattttcgaagacc PRIMER_LEFT_4=1460,20 PRIMER_RIGHT_4=1664,20 PRIMER_LEFT_4_TM=60.220 PRIMER_RIGHT_4_TM=59.910 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=205 = SEQUENCE_ID=Medicago Lyk3 mRNA picks primers overlapping one intron one pair SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=646 680 838 1041 1133 1228 1382 1469 1608 1706 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_THREE_PRIME_DISTANCE=10 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.186184 PRIMER_LEFT_0_PENALTY=0.110392 PRIMER_RIGHT_0_PENALTY=0.075792 PRIMER_LEFT_0_SEQUENCE=cgtggaaaggttgctgattt PRIMER_RIGHT_0_SEQUENCE=ttctggtggcatgtatccaa PRIMER_LEFT_0=1369,20 PRIMER_RIGHT_0=1470,20 PRIMER_LEFT_0_TM=60.110 PRIMER_RIGHT_0_TM=59.924 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=102 PRIMER_PAIR_1_PENALTY=0.310009 PRIMER_LEFT_1_PENALTY=0.219854 PRIMER_RIGHT_1_PENALTY=0.090155 PRIMER_LEFT_1_SEQUENCE=tgccaccagaatatgctcaa PRIMER_RIGHT_1_SEQUENCE=tccaccaattttcgaagacc PRIMER_LEFT_1=1460,20 PRIMER_RIGHT_1=1664,20 PRIMER_LEFT_1_TM=60.220 PRIMER_RIGHT_1_TM=59.910 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=205 PRIMER_PAIR_2_PENALTY=0.473092 PRIMER_LEFT_2_PENALTY=0.224191 PRIMER_RIGHT_2_PENALTY=0.248900 PRIMER_LEFT_2_SEQUENCE=tgaagggtcacttttcctcg PRIMER_RIGHT_2_SEQUENCE=caagccaaaatcagcaacct PRIMER_LEFT_2=1155,20 PRIMER_RIGHT_2=1395,20 PRIMER_LEFT_2_TM=60.224 PRIMER_RIGHT_2_TM=60.249 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.9000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=241 PRIMER_PAIR_3_PENALTY=0.589497 PRIMER_LEFT_3_PENALTY=0.046801 PRIMER_RIGHT_3_PENALTY=0.542697 PRIMER_LEFT_3_SEQUENCE=tacaggcattatggtggcaa PRIMER_RIGHT_3_SEQUENCE=aattgctgttttctcgcctc PRIMER_LEFT_3=906,20 PRIMER_RIGHT_3=1053,20 PRIMER_LEFT_3_TM=59.953 PRIMER_RIGHT_3_TM=59.457 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=10.0000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=148 PRIMER_PAIR_4_PENALTY=1.144151 PRIMER_LEFT_4_PENALTY=0.919960 PRIMER_RIGHT_4_PENALTY=0.224191 PRIMER_LEFT_4_SEQUENCE=agaggcgagaaaacagcaat PRIMER_RIGHT_4_SEQUENCE=cgaggaaaagtgacccttca PRIMER_LEFT_4=1033,20 PRIMER_RIGHT_4=1174,20 PRIMER_LEFT_4_TM=59.080 PRIMER_RIGHT_4_TM=60.224 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=142 = SEQUENCE_ID=Medicago Lyk3 mRNA picks primers overlapping one intron one pair SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=1133 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_THREE_PRIME_DISTANCE=10 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=1.110563 PRIMER_LEFT_0_PENALTY=0.046801 PRIMER_RIGHT_0_PENALTY=1.063762 PRIMER_LEFT_0_SEQUENCE=tacaggcattatggtggcaa PRIMER_RIGHT_0_SEQUENCE=atcaaccgcaccagattca PRIMER_LEFT_0=906,20 PRIMER_RIGHT_0=1142,19 PRIMER_LEFT_0_TM=59.953 PRIMER_RIGHT_0_TM=60.064 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=47.368 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=237 PRIMER_PAIR_1_PENALTY=1.176962 PRIMER_LEFT_1_PENALTY=1.066570 PRIMER_RIGHT_1_PENALTY=0.110392 PRIMER_LEFT_1_SEQUENCE=atcacttgaatctggtgcgg PRIMER_RIGHT_1_SEQUENCE=aaatcagcaacctttccacg PRIMER_LEFT_1=1118,20 PRIMER_RIGHT_1=1388,20 PRIMER_LEFT_1_TM=61.067 PRIMER_RIGHT_1_TM=60.110 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=11.7000 PRIMER_RIGHT_1_END_STABILITY=9.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=271 = primer3-2.3.6/test/primer_check_output0000664000077300007730000004236612231156245017046 0ustar ioanaioanaSEQUENCE_ID=00 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_INTERNAL_MIN_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_INTERNAL_MAX_SELF_ANY=100 PRIMER_INTERNAL_MAX_SELF_END=100 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_INTERNAL_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=02 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_PAIR_EXPLAIN=considered 2, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 PRIMER_PAIR_1_PENALTY=14.227051 PRIMER_LEFT_1_PENALTY=2.731673 PRIMER_RIGHT_1_PENALTY=11.495378 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT PRIMER_LEFT_1=2,25 PRIMER_RIGHT_1=54,25 PRIMER_LEFT_1_TM=62.732 PRIMER_RIGHT_1_TM=71.495 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=56.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=12.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=12.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=7.1000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=53 = SEQUENCE_ID=03 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=04 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, no internal oligo 2, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=05 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 2, no internal oligo 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=06 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=07 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=14.227051 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=54,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=12.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=7.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=53 = SEQUENCE_ID=08 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=14.227051 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=TGCGTCGACTGCAACGTACGTACGT PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=54,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=12.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=7.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=53 = SEQUENCE_ID=09 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 35, too many Ns 33, ok 2 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, no internal oligo 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=10 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no internal oligo 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=11 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAATTTGATTG SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_WARNING=Specified left primer < PRIMER_MIN_SIZE PRIMER_ERROR=Specified left primer not in sequence = SEQUENCE_ID=12 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_ERROR=Not specified to pick internal oligos but a specific internal oligo is provided = SEQUENCE_ID=13 SEQUENCE_TEMPLATE=nncacgtcaTCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=cacgtcatctcatcaaatttgattg SEQUENCE_PRIMER_REVCOMP=gaattgtttggaaatgacagtgagt PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=cacgtcaTCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = SEQUENCE_ID=14 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_PRIMER=CACGTCATctcatcaaattTGATTG SEQUENCE_PRIMER_REVCOMP=gaattgtttggaAATGACAGTGAGT PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_INTERNAL_EXPLAIN=considered 60, too many Ns 57, ok 3 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_INTERNAL_0_PENALTY=11.495378 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_INTERNAL_0_SEQUENCE=ACGTACGTACGTTGCAGTCGACGCA PRIMER_LEFT_0=2,25 PRIMER_RIGHT_0=81,25 PRIMER_INTERNAL_0=30,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_INTERNAL_0_TM=71.495 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_INTERNAL_0_GC_PERCENT=56.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_INTERNAL_0_SELF_ANY=12.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=80 = primer3-2.3.6/test/primer_new_tasks_th_output0000664000077300007730000024374312231156245020464 0ustar ioanaioanaPRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 SEQUENCE_ID=Check left only SEQUENCE_PRIMER=gtcctcgaacagctcagtcc PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 PRIMER_DNTP_CONC=0.0 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=1.013677 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_LEFT_0=0,20 PRIMER_LEFT_0_TM=61.014 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.8500 = SEQUENCE_ID=Check internal only SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_INTERNAL_0_PENALTY=8.869010 PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa PRIMER_INTERNAL_0=90,20 PRIMER_INTERNAL_0_TM=51.131 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 = SEQUENCE_ID=Check right only SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_TASK=check_primers PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=2.682567 PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_RIGHT_0=199,20 PRIMER_RIGHT_0_TM=57.317 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_END_STABILITY=2.8300 = SEQUENCE_ID=Pick the same primers as before SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.055158 PRIMER_LEFT_0_PENALTY=0.029777 PRIMER_RIGHT_0_PENALTY=0.025380 PRIMER_INTERNAL_0_PENALTY=0.088782 PRIMER_LEFT_0_SEQUENCE=acgtcaaattcggccacttc PRIMER_RIGHT_0_SEQUENCE=atccgaagatgtggaccgtt PRIMER_INTERNAL_0_SEQUENCE=cgccgcgtccatgtcatcga PRIMER_LEFT_0=652,20 PRIMER_RIGHT_0=811,20 PRIMER_INTERNAL_0=711,20 PRIMER_LEFT_0_TM=60.030 PRIMER_RIGHT_0_TM=60.025 PRIMER_INTERNAL_0_TM=59.911 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=65.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.95 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.0100 PRIMER_RIGHT_0_END_STABILITY=4.4400 PRIMER_PAIR_0_COMPL_ANY_TH=17.70 PRIMER_PAIR_0_COMPL_END_TH=17.70 PRIMER_PAIR_0_PRODUCT_SIZE=160 = SEQUENCE_ID=Check primers with sequence SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.696244 PRIMER_LEFT_0_PENALTY=1.013677 PRIMER_RIGHT_0_PENALTY=2.682567 PRIMER_INTERNAL_0_PENALTY=8.869010 PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa PRIMER_LEFT_0=198,20 PRIMER_RIGHT_0=315,20 PRIMER_INTERNAL_0=250,20 PRIMER_LEFT_0_TM=61.014 PRIMER_RIGHT_0_TM=57.317 PRIMER_INTERNAL_0_TM=51.131 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=2.8300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=118 = SEQUENCE_ID=Check primers without sequence PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_INTERNAL_OLIGO=ccacggatgaggaagaagaa SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.696244 PRIMER_LEFT_0_PENALTY=1.013677 PRIMER_RIGHT_0_PENALTY=2.682567 PRIMER_INTERNAL_0_PENALTY=8.869010 PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_INTERNAL_0_SEQUENCE=ccacggatgaggaagaagaa PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=199,20 PRIMER_INTERNAL_0=90,20 PRIMER_LEFT_0_TM=61.014 PRIMER_RIGHT_0_TM=57.317 PRIMER_INTERNAL_0_TM=51.131 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=2.8300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=200 = SEQUENCE_ID=Check primers without sequence and internal PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=5 PRIMER_TASK=check_primers SEQUENCE_PRIMER=gtcctcgaacagctcagtcc SEQUENCE_PRIMER_REVCOMP=gcgtttttattgccgttgtt PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.696244 PRIMER_LEFT_0_PENALTY=1.013677 PRIMER_RIGHT_0_PENALTY=2.682567 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=199,20 PRIMER_LEFT_0_TM=61.014 PRIMER_RIGHT_0_TM=57.317 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=2.8300 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=200 = SEQUENCE_ID=Pick three times the same primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.025380 PRIMER_RIGHT_0_PENALTY=0.025380 PRIMER_INTERNAL_0_PENALTY=0.042931 PRIMER_LEFT_0_SEQUENCE=aacggtccacatcttcggat PRIMER_RIGHT_0_SEQUENCE=atccgaagatgtggaccgtt PRIMER_INTERNAL_0_SEQUENCE=gccgcttccctcggacaacc PRIMER_LEFT_0=792,20 PRIMER_RIGHT_0=811,20 PRIMER_INTERNAL_0=891,20 PRIMER_LEFT_0_TM=60.025 PRIMER_RIGHT_0_TM=60.025 PRIMER_INTERNAL_0_TM=60.043 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=70.000 PRIMER_INTERNAL_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=34.89 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=45.87 PRIMER_LEFT_0_END_STABILITY=4.1800 PRIMER_RIGHT_0_END_STABILITY=4.4400 PRIMER_LEFT_1_PENALTY=0.025995 PRIMER_RIGHT_1_PENALTY=0.028504 PRIMER_INTERNAL_1_PENALTY=0.088782 PRIMER_LEFT_1_SEQUENCE=ctggtctcccatcgtcaact PRIMER_RIGHT_1_SEQUENCE=gatttgcaaagctcaccgga PRIMER_INTERNAL_1_SEQUENCE=cgccgcgtccatgtcatcga PRIMER_LEFT_1=107,20 PRIMER_RIGHT_1=1371,20 PRIMER_INTERNAL_1=711,20 PRIMER_LEFT_1_TM=60.026 PRIMER_RIGHT_1_TM=60.029 PRIMER_INTERNAL_1_TM=59.911 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=65.000 PRIMER_INTERNAL_1_SELF_ANY_TH=0.95 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=9.84 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=41.58 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=3.1600 PRIMER_RIGHT_1_END_STABILITY=5.1400 PRIMER_LEFT_2_PENALTY=0.028504 PRIMER_RIGHT_2_PENALTY=0.028504 PRIMER_INTERNAL_2_PENALTY=0.160958 PRIMER_LEFT_2_SEQUENCE=tccggtgagctttgcaaatc PRIMER_RIGHT_2_SEQUENCE=ttctgcatcggaaaaccagc PRIMER_INTERNAL_2_SEQUENCE=ccggaggtcaatcgccaccg PRIMER_LEFT_2=1352,20 PRIMER_RIGHT_2=1213,20 PRIMER_INTERNAL_2=839,20 PRIMER_LEFT_2_TM=60.029 PRIMER_RIGHT_2_TM=60.029 PRIMER_INTERNAL_2_TM=59.839 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_INTERNAL_2_GC_PERCENT=70.000 PRIMER_INTERNAL_2_SELF_ANY_TH=6.43 PRIMER_LEFT_2_SELF_ANY_TH=3.25 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=6.43 PRIMER_LEFT_2_SELF_END_TH=0.25 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=44.15 PRIMER_LEFT_2_END_STABILITY=2.1700 PRIMER_RIGHT_2_END_STABILITY=4.8500 PRIMER_LEFT_3_PENALTY=0.028504 PRIMER_RIGHT_3_PENALTY=0.029777 PRIMER_INTERNAL_3_PENALTY=0.180197 PRIMER_LEFT_3_SEQUENCE=gctggttttccgatgcagaa PRIMER_RIGHT_3_SEQUENCE=gaagtggccgaatttgacgt PRIMER_INTERNAL_3_SEQUENCE=acgccgcgtccatgtcatcg PRIMER_LEFT_3=1194,20 PRIMER_RIGHT_3=671,20 PRIMER_INTERNAL_3=710,20 PRIMER_LEFT_3_TM=60.029 PRIMER_RIGHT_3_TM=60.030 PRIMER_INTERNAL_3_TM=60.180 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_SELF_ANY_TH=15.26 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=44.71 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=27.20 PRIMER_LEFT_3_END_STABILITY=3.0200 PRIMER_RIGHT_3_END_STABILITY=4.3400 PRIMER_LEFT_4_PENALTY=0.029777 PRIMER_RIGHT_4_PENALTY=0.029874 PRIMER_INTERNAL_4_PENALTY=0.309918 PRIMER_LEFT_4_SEQUENCE=acgtcaaattcggccacttc PRIMER_RIGHT_4_SEQUENCE=tgaagtggccgaatttgacg PRIMER_INTERNAL_4_SEQUENCE=ggtgctgggggtgcgcataa PRIMER_LEFT_4=652,20 PRIMER_RIGHT_4=672,20 PRIMER_INTERNAL_4=522,20 PRIMER_LEFT_4_TM=60.030 PRIMER_RIGHT_4_TM=60.030 PRIMER_INTERNAL_4_TM=59.690 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=65.000 PRIMER_INTERNAL_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=39.06 PRIMER_LEFT_4_END_STABILITY=3.0100 PRIMER_RIGHT_4_END_STABILITY=4.3500 = SEQUENCE_ID=Make a list of a small range SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatga PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=20 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=20 PRIMER_RIGHT_NUM_RETURNED=20 PRIMER_INTERNAL_NUM_RETURNED=4 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=1.380503 PRIMER_RIGHT_0_PENALTY=1.354059 PRIMER_INTERNAL_0_PENALTY=8.328764 PRIMER_LEFT_0_SEQUENCE=tggacatggatgccatcaatg PRIMER_RIGHT_0_SEQUENCE=tgatggcatccatgtccatc PRIMER_INTERNAL_0_SEQUENCE=tggatttgatggacatggatgccatca PRIMER_LEFT_0=10,21 PRIMER_RIGHT_0=27,20 PRIMER_INTERNAL_0=1,27 PRIMER_LEFT_0_TM=59.619 PRIMER_RIGHT_0_TM=58.646 PRIMER_INTERNAL_0_TM=58.671 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=44.444 PRIMER_INTERNAL_0_SELF_ANY_TH=23.10 PRIMER_LEFT_0_SELF_ANY_TH=5.26 PRIMER_RIGHT_0_SELF_ANY_TH=23.68 PRIMER_INTERNAL_0_SELF_END_TH=22.04 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=23.68 PRIMER_LEFT_0_HAIRPIN_TH=44.12 PRIMER_RIGHT_0_HAIRPIN_TH=46.18 PRIMER_INTERNAL_0_HAIRPIN_TH=46.56 PRIMER_LEFT_0_END_STABILITY=2.8200 PRIMER_RIGHT_0_END_STABILITY=3.5100 PRIMER_LEFT_1_PENALTY=1.591479 PRIMER_RIGHT_1_PENALTY=1.354059 PRIMER_INTERNAL_1_PENALTY=8.471374 PRIMER_LEFT_1_SEQUENCE=tttgatggacatggatgcca PRIMER_RIGHT_1_SEQUENCE=gatggcatccatgtccatca PRIMER_INTERNAL_1_SEQUENCE=ggatttgatggacatggatgccatca PRIMER_LEFT_1=5,20 PRIMER_RIGHT_1=26,20 PRIMER_INTERNAL_1=2,26 PRIMER_LEFT_1_TM=58.409 PRIMER_RIGHT_1_TM=58.646 PRIMER_INTERNAL_1_TM=57.529 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=46.154 PRIMER_INTERNAL_1_SELF_ANY_TH=23.10 PRIMER_LEFT_1_SELF_ANY_TH=5.74 PRIMER_RIGHT_1_SELF_ANY_TH=24.61 PRIMER_INTERNAL_1_SELF_END_TH=22.04 PRIMER_LEFT_1_SELF_END_TH=5.74 PRIMER_RIGHT_1_SELF_END_TH=5.95 PRIMER_LEFT_1_HAIRPIN_TH=38.48 PRIMER_RIGHT_1_HAIRPIN_TH=46.18 PRIMER_INTERNAL_1_HAIRPIN_TH=46.56 PRIMER_LEFT_1_END_STABILITY=4.9200 PRIMER_RIGHT_1_END_STABILITY=3.0700 PRIMER_LEFT_2_PENALTY=1.657646 PRIMER_RIGHT_2_PENALTY=1.380503 PRIMER_INTERNAL_2_PENALTY=8.471374 PRIMER_LEFT_2_SEQUENCE=ggacatggatgccatcaatga PRIMER_RIGHT_2_SEQUENCE=cattgatggcatccatgtcca PRIMER_INTERNAL_2_SEQUENCE=tggatttgatggacatggatgccatc PRIMER_LEFT_2=11,21 PRIMER_RIGHT_2=30,21 PRIMER_INTERNAL_2=1,26 PRIMER_LEFT_2_TM=59.342 PRIMER_RIGHT_2_TM=59.619 PRIMER_INTERNAL_2_TM=57.529 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_INTERNAL_2_GC_PERCENT=46.154 PRIMER_INTERNAL_2_SELF_ANY_TH=18.44 PRIMER_LEFT_2_SELF_ANY_TH=26.38 PRIMER_RIGHT_2_SELF_ANY_TH=11.74 PRIMER_INTERNAL_2_SELF_END_TH=18.44 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=5.68 PRIMER_LEFT_2_HAIRPIN_TH=44.12 PRIMER_RIGHT_2_HAIRPIN_TH=46.18 PRIMER_INTERNAL_2_HAIRPIN_TH=44.20 PRIMER_LEFT_2_END_STABILITY=2.5700 PRIMER_RIGHT_2_END_STABILITY=4.0200 PRIMER_LEFT_3_PENALTY=1.819575 PRIMER_RIGHT_3_PENALTY=1.591479 PRIMER_INTERNAL_3_PENALTY=9.328375 PRIMER_LEFT_3_SEQUENCE=tggacatggatgccatcaat PRIMER_RIGHT_3_SEQUENCE=tggcatccatgtccatcaaa PRIMER_INTERNAL_3_SEQUENCE=atggatttgatggacatggatgccatc PRIMER_LEFT_3=10,20 PRIMER_RIGHT_3=24,20 PRIMER_INTERNAL_3=0,27 PRIMER_LEFT_3_TM=58.180 PRIMER_RIGHT_3_TM=58.409 PRIMER_INTERNAL_3_TM=57.672 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_INTERNAL_3_GC_PERCENT=44.444 PRIMER_INTERNAL_3_SELF_ANY_TH=18.44 PRIMER_LEFT_3_SELF_ANY_TH=5.26 PRIMER_RIGHT_3_SELF_ANY_TH=5.19 PRIMER_INTERNAL_3_SELF_END_TH=18.44 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=44.12 PRIMER_RIGHT_3_HAIRPIN_TH=32.64 PRIMER_INTERNAL_3_HAIRPIN_TH=44.20 PRIMER_LEFT_3_END_STABILITY=2.5700 PRIMER_RIGHT_3_END_STABILITY=2.6900 PRIMER_LEFT_4_PENALTY=1.819575 PRIMER_RIGHT_4_PENALTY=1.657646 PRIMER_LEFT_4_SEQUENCE=atggacatggatgccatcaa PRIMER_RIGHT_4_SEQUENCE=tcattgatggcatccatgtcc PRIMER_LEFT_4=9,20 PRIMER_RIGHT_4=31,21 PRIMER_LEFT_4_TM=58.180 PRIMER_RIGHT_4_TM=59.342 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY_TH=9.52 PRIMER_RIGHT_4_SELF_ANY_TH=14.32 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=44.12 PRIMER_RIGHT_4_HAIRPIN_TH=46.18 PRIMER_LEFT_4_END_STABILITY=2.5700 PRIMER_RIGHT_4_END_STABILITY=4.0200 PRIMER_LEFT_5_PENALTY=2.024122 PRIMER_RIGHT_5_PENALTY=1.657646 PRIMER_LEFT_5_SEQUENCE=gatttgatggacatggatgcca PRIMER_RIGHT_5_SEQUENCE=ttgatggcatccatgtccatc PRIMER_LEFT_5=3,22 PRIMER_RIGHT_5=28,21 PRIMER_LEFT_5_TM=59.976 PRIMER_RIGHT_5_TM=59.342 PRIMER_LEFT_5_GC_PERCENT=45.455 PRIMER_RIGHT_5_GC_PERCENT=47.619 PRIMER_LEFT_5_SELF_ANY_TH=5.74 PRIMER_RIGHT_5_SELF_ANY_TH=23.68 PRIMER_LEFT_5_SELF_END_TH=5.74 PRIMER_RIGHT_5_SELF_END_TH=23.68 PRIMER_LEFT_5_HAIRPIN_TH=38.48 PRIMER_RIGHT_5_HAIRPIN_TH=46.18 PRIMER_LEFT_5_END_STABILITY=4.9200 PRIMER_RIGHT_5_END_STABILITY=3.5100 PRIMER_LEFT_6_PENALTY=2.024122 PRIMER_RIGHT_6_PENALTY=1.657646 PRIMER_LEFT_6_SEQUENCE=tggatttgatggacatggatgc PRIMER_RIGHT_6_SEQUENCE=gatggcatccatgtccatcaa PRIMER_LEFT_6=1,22 PRIMER_RIGHT_6=26,21 PRIMER_LEFT_6_TM=59.976 PRIMER_RIGHT_6_TM=59.342 PRIMER_LEFT_6_GC_PERCENT=45.455 PRIMER_RIGHT_6_GC_PERCENT=47.619 PRIMER_LEFT_6_SELF_ANY_TH=0.00 PRIMER_RIGHT_6_SELF_ANY_TH=24.61 PRIMER_LEFT_6_SELF_END_TH=0.00 PRIMER_RIGHT_6_SELF_END_TH=2.31 PRIMER_LEFT_6_HAIRPIN_TH=0.00 PRIMER_RIGHT_6_HAIRPIN_TH=46.18 PRIMER_LEFT_6_END_STABILITY=3.9100 PRIMER_RIGHT_6_END_STABILITY=2.5700 PRIMER_LEFT_7_PENALTY=2.041530 PRIMER_RIGHT_7_PENALTY=1.819575 PRIMER_LEFT_7_SEQUENCE=atggacatggatgccatcaatg PRIMER_RIGHT_7_SEQUENCE=attgatggcatccatgtcca PRIMER_LEFT_7=9,22 PRIMER_RIGHT_7=29,20 PRIMER_LEFT_7_TM=60.042 PRIMER_RIGHT_7_TM=58.180 PRIMER_LEFT_7_GC_PERCENT=45.455 PRIMER_RIGHT_7_GC_PERCENT=45.000 PRIMER_LEFT_7_SELF_ANY_TH=9.52 PRIMER_RIGHT_7_SELF_ANY_TH=5.68 PRIMER_LEFT_7_SELF_END_TH=0.00 PRIMER_RIGHT_7_SELF_END_TH=5.68 PRIMER_LEFT_7_HAIRPIN_TH=44.12 PRIMER_RIGHT_7_HAIRPIN_TH=46.18 PRIMER_LEFT_7_END_STABILITY=2.8200 PRIMER_RIGHT_7_END_STABILITY=4.0200 PRIMER_LEFT_8_PENALTY=2.090718 PRIMER_RIGHT_8_PENALTY=1.819575 PRIMER_LEFT_8_SEQUENCE=atttgatggacatggatgcca PRIMER_RIGHT_8_SEQUENCE=ttgatggcatccatgtccat PRIMER_LEFT_8=4,21 PRIMER_RIGHT_8=28,20 PRIMER_LEFT_8_TM=58.909 PRIMER_RIGHT_8_TM=58.180 PRIMER_LEFT_8_GC_PERCENT=42.857 PRIMER_RIGHT_8_GC_PERCENT=45.000 PRIMER_LEFT_8_SELF_ANY_TH=5.74 PRIMER_RIGHT_8_SELF_ANY_TH=13.31 PRIMER_LEFT_8_SELF_END_TH=5.74 PRIMER_RIGHT_8_SELF_END_TH=13.31 PRIMER_LEFT_8_HAIRPIN_TH=38.48 PRIMER_RIGHT_8_HAIRPIN_TH=46.18 PRIMER_LEFT_8_END_STABILITY=4.9200 PRIMER_RIGHT_8_END_STABILITY=3.4100 PRIMER_LEFT_9_PENALTY=2.189272 PRIMER_RIGHT_9_PENALTY=1.819575 PRIMER_LEFT_9_SEQUENCE=ggacatggatgccatcaatg PRIMER_RIGHT_9_SEQUENCE=atggcatccatgtccatcaa PRIMER_LEFT_9=11,20 PRIMER_RIGHT_9=25,20 PRIMER_LEFT_9_TM=57.811 PRIMER_RIGHT_9_TM=58.180 PRIMER_LEFT_9_GC_PERCENT=50.000 PRIMER_RIGHT_9_GC_PERCENT=45.000 PRIMER_LEFT_9_SELF_ANY_TH=0.00 PRIMER_RIGHT_9_SELF_ANY_TH=9.50 PRIMER_LEFT_9_SELF_END_TH=0.00 PRIMER_RIGHT_9_SELF_END_TH=0.00 PRIMER_LEFT_9_HAIRPIN_TH=44.12 PRIMER_RIGHT_9_HAIRPIN_TH=44.27 PRIMER_LEFT_9_END_STABILITY=2.8200 PRIMER_RIGHT_9_END_STABILITY=2.5700 PRIMER_LEFT_10_PENALTY=2.306792 PRIMER_RIGHT_10_PENALTY=2.024122 PRIMER_LEFT_10_SEQUENCE=atggacatggatgccatcaat PRIMER_RIGHT_10_SEQUENCE=gatggcatccatgtccatcaaa PRIMER_LEFT_10=9,21 PRIMER_RIGHT_10=26,22 PRIMER_LEFT_10_TM=58.693 PRIMER_RIGHT_10_TM=59.976 PRIMER_LEFT_10_GC_PERCENT=42.857 PRIMER_RIGHT_10_GC_PERCENT=45.455 PRIMER_LEFT_10_SELF_ANY_TH=9.52 PRIMER_RIGHT_10_SELF_ANY_TH=24.61 PRIMER_LEFT_10_SELF_END_TH=0.00 PRIMER_RIGHT_10_SELF_END_TH=2.05 PRIMER_LEFT_10_HAIRPIN_TH=44.12 PRIMER_RIGHT_10_HAIRPIN_TH=46.18 PRIMER_LEFT_10_END_STABILITY=2.5700 PRIMER_RIGHT_10_END_STABILITY=2.6900 PRIMER_LEFT_11_PENALTY=2.632409 PRIMER_RIGHT_11_PENALTY=2.024122 PRIMER_LEFT_11_SEQUENCE=ggatttgatggacatggatgcc PRIMER_RIGHT_11_SEQUENCE=tggcatccatgtccatcaaatc PRIMER_LEFT_11=2,22 PRIMER_RIGHT_11=24,22 PRIMER_LEFT_11_TM=60.632 PRIMER_RIGHT_11_TM=59.976 PRIMER_LEFT_11_GC_PERCENT=50.000 PRIMER_RIGHT_11_GC_PERCENT=45.455 PRIMER_LEFT_11_SELF_ANY_TH=0.00 PRIMER_RIGHT_11_SELF_ANY_TH=5.19 PRIMER_LEFT_11_SELF_END_TH=0.00 PRIMER_RIGHT_11_SELF_END_TH=0.00 PRIMER_LEFT_11_HAIRPIN_TH=0.00 PRIMER_RIGHT_11_HAIRPIN_TH=32.64 PRIMER_LEFT_11_END_STABILITY=4.4000 PRIMER_RIGHT_11_END_STABILITY=2.1700 PRIMER_LEFT_12_PENALTY=2.738369 PRIMER_RIGHT_12_PENALTY=2.024122 PRIMER_LEFT_12_SEQUENCE=gatttgatggacatggatgcc PRIMER_RIGHT_12_SEQUENCE=gcatccatgtccatcaaatcca PRIMER_LEFT_12=3,21 PRIMER_RIGHT_12=22,22 PRIMER_LEFT_12_TM=58.262 PRIMER_RIGHT_12_TM=59.976 PRIMER_LEFT_12_GC_PERCENT=47.619 PRIMER_RIGHT_12_GC_PERCENT=45.455 PRIMER_LEFT_12_SELF_ANY_TH=0.00 PRIMER_RIGHT_12_SELF_ANY_TH=0.00 PRIMER_LEFT_12_SELF_END_TH=0.00 PRIMER_RIGHT_12_SELF_END_TH=0.00 PRIMER_LEFT_12_HAIRPIN_TH=0.00 PRIMER_RIGHT_12_HAIRPIN_TH=0.00 PRIMER_LEFT_12_END_STABILITY=4.4000 PRIMER_RIGHT_12_END_STABILITY=3.4100 PRIMER_LEFT_13_PENALTY=2.738369 PRIMER_RIGHT_13_PENALTY=2.041530 PRIMER_LEFT_13_SEQUENCE=ggatttgatggacatggatgc PRIMER_RIGHT_13_SEQUENCE=cattgatggcatccatgtccat PRIMER_LEFT_13=2,21 PRIMER_RIGHT_13=30,22 PRIMER_LEFT_13_TM=58.262 PRIMER_RIGHT_13_TM=60.042 PRIMER_LEFT_13_GC_PERCENT=47.619 PRIMER_RIGHT_13_GC_PERCENT=45.455 PRIMER_LEFT_13_SELF_ANY_TH=0.00 PRIMER_RIGHT_13_SELF_ANY_TH=13.31 PRIMER_LEFT_13_SELF_END_TH=0.00 PRIMER_RIGHT_13_SELF_END_TH=13.31 PRIMER_LEFT_13_HAIRPIN_TH=0.00 PRIMER_RIGHT_13_HAIRPIN_TH=46.18 PRIMER_LEFT_13_END_STABILITY=3.9100 PRIMER_RIGHT_13_END_STABILITY=3.4100 PRIMER_LEFT_14_PENALTY=2.954042 PRIMER_RIGHT_14_PENALTY=2.090718 PRIMER_LEFT_14_SEQUENCE=atttgatggacatggatgcc PRIMER_RIGHT_14_SEQUENCE=atggcatccatgtccatcaaa PRIMER_LEFT_14=4,20 PRIMER_RIGHT_14=25,21 PRIMER_LEFT_14_TM=57.046 PRIMER_RIGHT_14_TM=58.909 PRIMER_LEFT_14_GC_PERCENT=45.000 PRIMER_RIGHT_14_GC_PERCENT=42.857 PRIMER_LEFT_14_SELF_ANY_TH=0.00 PRIMER_RIGHT_14_SELF_ANY_TH=9.50 PRIMER_LEFT_14_SELF_END_TH=0.00 PRIMER_RIGHT_14_SELF_END_TH=0.00 PRIMER_LEFT_14_HAIRPIN_TH=0.00 PRIMER_RIGHT_14_HAIRPIN_TH=44.27 PRIMER_LEFT_14_END_STABILITY=4.4000 PRIMER_RIGHT_14_END_STABILITY=2.6900 PRIMER_LEFT_15_PENALTY=3.043060 PRIMER_RIGHT_15_PENALTY=2.090718 PRIMER_LEFT_15_SEQUENCE=tggacatggatgccatcaatga PRIMER_RIGHT_15_SEQUENCE=tggcatccatgtccatcaaat PRIMER_LEFT_15=10,22 PRIMER_RIGHT_15=24,21 PRIMER_LEFT_15_TM=61.043 PRIMER_RIGHT_15_TM=58.909 PRIMER_LEFT_15_GC_PERCENT=45.455 PRIMER_RIGHT_15_GC_PERCENT=42.857 PRIMER_LEFT_15_SELF_ANY_TH=26.38 PRIMER_RIGHT_15_SELF_ANY_TH=5.19 PRIMER_LEFT_15_SELF_END_TH=4.73 PRIMER_RIGHT_15_SELF_END_TH=0.00 PRIMER_LEFT_15_HAIRPIN_TH=44.12 PRIMER_RIGHT_15_HAIRPIN_TH=32.64 PRIMER_LEFT_15_END_STABILITY=2.5700 PRIMER_RIGHT_15_END_STABILITY=2.3200 PRIMER_LEFT_16_PENALTY=3.365788 PRIMER_RIGHT_16_PENALTY=2.189272 PRIMER_LEFT_16_SEQUENCE=atggatttgatggacatggatgc PRIMER_RIGHT_16_SEQUENCE=cattgatggcatccatgtcc PRIMER_LEFT_16=0,23 PRIMER_RIGHT_16=30,20 PRIMER_LEFT_16_TM=60.366 PRIMER_RIGHT_16_TM=57.811 PRIMER_LEFT_16_GC_PERCENT=43.478 PRIMER_RIGHT_16_GC_PERCENT=50.000 PRIMER_LEFT_16_SELF_ANY_TH=0.00 PRIMER_RIGHT_16_SELF_ANY_TH=11.74 PRIMER_LEFT_16_SELF_END_TH=0.00 PRIMER_RIGHT_16_SELF_END_TH=0.00 PRIMER_LEFT_16_HAIRPIN_TH=0.00 PRIMER_RIGHT_16_HAIRPIN_TH=46.18 PRIMER_LEFT_16_END_STABILITY=3.9100 PRIMER_RIGHT_16_END_STABILITY=4.0200 PRIMER_LEFT_17_PENALTY=3.382273 PRIMER_RIGHT_17_PENALTY=2.223627 PRIMER_LEFT_17_SEQUENCE=tggacatggatgccatcaa PRIMER_RIGHT_17_SEQUENCE=attgatggcatccatgtccatc PRIMER_LEFT_17=10,19 PRIMER_RIGHT_17=29,22 PRIMER_LEFT_17_TM=57.618 PRIMER_RIGHT_17_TM=59.776 PRIMER_LEFT_17_GC_PERCENT=47.368 PRIMER_RIGHT_17_GC_PERCENT=45.455 PRIMER_LEFT_17_SELF_ANY_TH=5.26 PRIMER_RIGHT_17_SELF_ANY_TH=23.68 PRIMER_LEFT_17_SELF_END_TH=0.00 PRIMER_RIGHT_17_SELF_END_TH=23.68 PRIMER_LEFT_17_HAIRPIN_TH=44.12 PRIMER_RIGHT_17_HAIRPIN_TH=46.18 PRIMER_LEFT_17_END_STABILITY=2.5700 PRIMER_RIGHT_17_END_STABILITY=3.5100 PRIMER_LEFT_18_PENALTY=3.382273 PRIMER_RIGHT_18_PENALTY=2.306792 PRIMER_LEFT_18_SEQUENCE=ttgatggacatggatgcca PRIMER_RIGHT_18_SEQUENCE=attgatggcatccatgtccat PRIMER_LEFT_18=6,19 PRIMER_RIGHT_18=29,21 PRIMER_LEFT_18_TM=57.618 PRIMER_RIGHT_18_TM=58.693 PRIMER_LEFT_18_GC_PERCENT=47.368 PRIMER_RIGHT_18_GC_PERCENT=42.857 PRIMER_LEFT_18_SELF_ANY_TH=5.74 PRIMER_RIGHT_18_SELF_ANY_TH=13.31 PRIMER_LEFT_18_SELF_END_TH=5.74 PRIMER_RIGHT_18_SELF_END_TH=13.31 PRIMER_LEFT_18_HAIRPIN_TH=38.48 PRIMER_RIGHT_18_HAIRPIN_TH=46.18 PRIMER_LEFT_18_END_STABILITY=4.9200 PRIMER_RIGHT_18_END_STABILITY=3.4100 PRIMER_LEFT_19_PENALTY=3.625785 PRIMER_RIGHT_19_PENALTY=2.632337 PRIMER_LEFT_19_SEQUENCE=atggacatggatgccatca PRIMER_RIGHT_19_SEQUENCE=atggcatccatgtccatcaaat PRIMER_LEFT_19=9,19 PRIMER_RIGHT_19=25,22 PRIMER_LEFT_19_TM=57.374 PRIMER_RIGHT_19_TM=59.368 PRIMER_LEFT_19_GC_PERCENT=47.368 PRIMER_RIGHT_19_GC_PERCENT=40.909 PRIMER_LEFT_19_SELF_ANY_TH=9.52 PRIMER_RIGHT_19_SELF_ANY_TH=9.50 PRIMER_LEFT_19_SELF_END_TH=0.00 PRIMER_RIGHT_19_SELF_END_TH=0.00 PRIMER_LEFT_19_HAIRPIN_TH=44.12 PRIMER_RIGHT_19_HAIRPIN_TH=44.27 PRIMER_LEFT_19_END_STABILITY=3.0700 PRIMER_RIGHT_19_END_STABILITY=2.3200 = SEQUENCE_ID=Make a list of a small range SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatga PRIMER_LOWERCASE_MASKING=0 PRIMER_TASK=pick_primer_list PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 PRIMER_NUM_RETURN=2 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=1.354059 PRIMER_RIGHT_0_SEQUENCE=tgatggcatccatgtccatc PRIMER_RIGHT_0=27,20 PRIMER_RIGHT_0_TM=58.646 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_SELF_ANY_TH=23.68 PRIMER_RIGHT_0_SELF_END_TH=23.68 PRIMER_RIGHT_0_HAIRPIN_TH=46.18 PRIMER_RIGHT_0_END_STABILITY=3.5100 PRIMER_RIGHT_1_PENALTY=1.354059 PRIMER_RIGHT_1_SEQUENCE=gatggcatccatgtccatca PRIMER_RIGHT_1=26,20 PRIMER_RIGHT_1_TM=58.646 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_SELF_ANY_TH=24.61 PRIMER_RIGHT_1_SELF_END_TH=5.95 PRIMER_RIGHT_1_HAIRPIN_TH=46.18 PRIMER_RIGHT_1_END_STABILITY=3.0700 = SEQUENCE_ID=Check left only too small SEQUENCE_PRIMER=gtcctcgaaca PRIMER_TASK=check_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=Specified left primer < PRIMER_MIN_SIZE; PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=33.747070 PRIMER_LEFT_0_PROBLEMS= Temperature too low; Too short; PRIMER_LEFT_0_SEQUENCE=gtcctcgaaca PRIMER_LEFT_0=0,11 PRIMER_LEFT_0_TM=35.253 PRIMER_LEFT_0_GC_PERCENT=54.545 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.1800 = SEQUENCE_ID=Check left only too big SEQUENCE_PRIMER=tgacctccacttctctggacacagctccctcaccaa PRIMER_TASK=check_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=Specified left primer > PRIMER_MAX_SIZE; PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=31.449855 PRIMER_LEFT_0_PROBLEMS= Temperature too high; Too long; PRIMER_LEFT_0_SEQUENCE=tgacctccacttctctggacacagctccctcaccaa PRIMER_LEFT_0=0,36 PRIMER_LEFT_0_TM=75.450 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=45.07 PRIMER_LEFT_0_END_STABILITY=3.6700 = SEQUENCE_ID=Pick sequencing primers no target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=50 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=4 PRIMER_RIGHT_NUM_RETURNED=4 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.692905 PRIMER_RIGHT_0_PENALTY=0.098220 PRIMER_LEFT_0_SEQUENCE=ggatgccatcaatgacctcc PRIMER_RIGHT_0_SEQUENCE=tgtcctcgatgatggtgtcc PRIMER_LEFT_0=17,20 PRIMER_RIGHT_0=189,20 PRIMER_LEFT_0_TM=59.307 PRIMER_RIGHT_0_TM=60.098 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=0.26 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.3000 PRIMER_RIGHT_0_END_STABILITY=4.0200 PRIMER_LEFT_1_PENALTY=1.038565 PRIMER_RIGHT_1_PENALTY=0.259988 PRIMER_LEFT_1_SEQUENCE=agggctaaaactagtgcacct PRIMER_RIGHT_1_SEQUENCE=tgatggcttgattggctgtg PRIMER_LEFT_1=338,21 PRIMER_RIGHT_1=690,20 PRIMER_LEFT_1_TM=59.961 PRIMER_RIGHT_1_TM=59.740 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY_TH=9.98 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=8.91 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=4.0000 PRIMER_RIGHT_1_END_STABILITY=3.6600 PRIMER_LEFT_2_PENALTY=0.309295 PRIMER_RIGHT_2_PENALTY=0.028504 PRIMER_LEFT_2_SEQUENCE=gcattatcgcggacaggaac PRIMER_RIGHT_2_SEQUENCE=ttctgcatcggaaaaccagc PRIMER_LEFT_2=816,20 PRIMER_RIGHT_2=1213,20 PRIMER_LEFT_2_TM=60.309 PRIMER_RIGHT_2_TM=60.029 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=3.6200 PRIMER_RIGHT_2_END_STABILITY=4.8500 PRIMER_LEFT_3_PENALTY=0.270229 PRIMER_RIGHT_3_PENALTY=3.267431 PRIMER_LEFT_3_SEQUENCE=ggtgaggtagggtttagggg PRIMER_RIGHT_3_SEQUENCE=tgaagaagaacaagtccaaaggg PRIMER_LEFT_3=1329,20 PRIMER_RIGHT_3=1511,23 PRIMER_LEFT_3_TM=59.730 PRIMER_RIGHT_3_TM=59.733 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=4.7900 PRIMER_RIGHT_3_END_STABILITY=3.9500 = SEQUENCE_ID=Pick sequencing primers small target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=450,37 PRIMER_NUM_RETURN=50 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.808071 PRIMER_RIGHT_0_PENALTY=0.029777 PRIMER_LEFT_0_SEQUENCE=cacaacaacaacaacggcaa PRIMER_RIGHT_0_SEQUENCE=gaagtggccgaatttgacgt PRIMER_LEFT_0=287,20 PRIMER_RIGHT_0=671,20 PRIMER_LEFT_0_TM=59.192 PRIMER_RIGHT_0_TM=60.030 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.5200 PRIMER_RIGHT_0_END_STABILITY=4.3400 = SEQUENCE_ID=Pick sequencing primers left only SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=150,800 PRIMER_NUM_RETURN=50 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.692905 PRIMER_LEFT_0_SEQUENCE=ggatgccatcaatgacctcc PRIMER_LEFT_0=17,20 PRIMER_LEFT_0_TM=59.307 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=0.26 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.3000 PRIMER_LEFT_1_PENALTY=0.097666 PRIMER_LEFT_1_SEQUENCE=ccgaagcccttcatggacta PRIMER_LEFT_1=496,20 PRIMER_LEFT_1_TM=60.098 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY_TH=9.08 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=40.00 PRIMER_LEFT_1_END_STABILITY=2.5900 = SEQUENCE_ID=Pick sequencing primers right only SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 SEQUENCE_TARGET=150,800 PRIMER_NUM_RETURN=50 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.113686 PRIMER_RIGHT_0_SEQUENCE=tggaaagcagcaagtgtgtc PRIMER_RIGHT_0=628,20 PRIMER_RIGHT_0_TM=59.886 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_END_STABILITY=3.6700 PRIMER_RIGHT_1_PENALTY=0.112929 PRIMER_RIGHT_1_SEQUENCE=ccaacgtcacaagctttgga PRIMER_RIGHT_1=1101,20 PRIMER_RIGHT_1_TM=59.887 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_SELF_ANY_TH=7.62 PRIMER_RIGHT_1_SELF_END_TH=2.46 PRIMER_RIGHT_1_HAIRPIN_TH=36.05 PRIMER_RIGHT_1_END_STABILITY=3.5300 = SEQUENCE_ID=Pick sequencing primers three target SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=350,37 450,100 850,500 1200,100 PRIMER_NUM_RETURN=50 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.098220 PRIMER_RIGHT_0_PENALTY=0.042322 PRIMER_LEFT_0_SEQUENCE=ggacaccatcatcgaggaca PRIMER_RIGHT_0_SEQUENCE=gttattatgcgcacccccag PRIMER_LEFT_0=170,20 PRIMER_RIGHT_0=545,20 PRIMER_LEFT_0_TM=60.098 PRIMER_RIGHT_0_TM=59.958 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.55 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.0200 PRIMER_RIGHT_0_END_STABILITY=4.4500 PRIMER_LEFT_1_PENALTY=0.808071 PRIMER_RIGHT_1_PENALTY=0.044209 PRIMER_LEFT_1_SEQUENCE=cacaacaacaacaacggcaa PRIMER_RIGHT_1_SEQUENCE=ttgattggctgtgaagtggc PRIMER_LEFT_1=287,20 PRIMER_RIGHT_1=683,20 PRIMER_LEFT_1_TM=59.192 PRIMER_RIGHT_1_TM=59.956 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=4.5200 PRIMER_RIGHT_1_END_STABILITY=5.0100 PRIMER_LEFT_2_PENALTY=0.029777 PRIMER_RIGHT_2_PENALTY=0.252674 PRIMER_LEFT_2_SEQUENCE=acgtcaaattcggccacttc PRIMER_RIGHT_2_SEQUENCE=aacgaagcaactgattccgg PRIMER_LEFT_2=652,20 PRIMER_RIGHT_2=1057,20 PRIMER_LEFT_2_TM=60.030 PRIMER_RIGHT_2_TM=59.747 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=5.77 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=37.93 PRIMER_LEFT_2_END_STABILITY=3.0100 PRIMER_RIGHT_2_END_STABILITY=5.1400 PRIMER_LEFT_3_PENALTY=2.108988 PRIMER_RIGHT_3_PENALTY=3.267431 PRIMER_LEFT_3_SEQUENCE=gtggaaaggttcatggactcac PRIMER_RIGHT_3_SEQUENCE=tgaagaagaacaagtccaaaggg PRIMER_LEFT_3=1137,22 PRIMER_RIGHT_3=1511,23 PRIMER_LEFT_3_TM=60.109 PRIMER_RIGHT_3_TM=59.733 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY_TH=3.06 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=2.37 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=3.5100 PRIMER_RIGHT_3_END_STABILITY=3.9500 PRIMER_LEFT_4_PENALTY=0.252674 PRIMER_RIGHT_4_PENALTY=0.169561 PRIMER_LEFT_4_SEQUENCE=ccggaatcagttgcttcgtt PRIMER_RIGHT_4_SEQUENCE=tactacccacacccacctct PRIMER_LEFT_4=1038,20 PRIMER_RIGHT_4=1443,20 PRIMER_LEFT_4_TM=59.747 PRIMER_RIGHT_4_TM=60.170 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=3.8500 PRIMER_RIGHT_4_END_STABILITY=3.6900 = SEQUENCE_ID=Pick fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 SEQUENCE_FORCE_LEFT_END=34 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=2.220283 PRIMER_LEFT_0_PENALTY=2.122953 PRIMER_RIGHT_0_PENALTY=0.097330 PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_0_SEQUENCE=aaaggtgtcccagttgacga PRIMER_LEFT_0=15,20 PRIMER_RIGHT_0=137,20 PRIMER_LEFT_0_TM=57.877 PRIMER_RIGHT_0_TM=60.097 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY_TH=0.82 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=41.92 PRIMER_RIGHT_0_HAIRPIN_TH=38.67 PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=4.2000 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=123 PRIMER_PAIR_1_PENALTY=2.221173 PRIMER_LEFT_1_PENALTY=2.122953 PRIMER_RIGHT_1_PENALTY=0.098220 PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_1_SEQUENCE=gtcctcgatgatggtgtcca PRIMER_LEFT_1=15,20 PRIMER_RIGHT_1=188,20 PRIMER_LEFT_1_TM=57.877 PRIMER_RIGHT_1_TM=60.098 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY_TH=0.82 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=41.92 PRIMER_RIGHT_1_HAIRPIN_TH=38.57 PRIMER_LEFT_1_END_STABILITY=3.8500 PRIMER_RIGHT_1_END_STABILITY=4.0200 PRIMER_PAIR_1_COMPL_ANY_TH=19.50 PRIMER_PAIR_1_COMPL_END_TH=18.10 PRIMER_PAIR_1_PRODUCT_SIZE=174 PRIMER_PAIR_2_PENALTY=2.221173 PRIMER_LEFT_2_PENALTY=2.122953 PRIMER_RIGHT_2_PENALTY=0.098220 PRIMER_LEFT_2_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_2_SEQUENCE=tgtcctcgatgatggtgtcc PRIMER_LEFT_2=15,20 PRIMER_RIGHT_2=189,20 PRIMER_LEFT_2_TM=57.877 PRIMER_RIGHT_2_TM=60.098 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY_TH=0.82 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=41.92 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=3.8500 PRIMER_RIGHT_2_END_STABILITY=4.0200 PRIMER_PAIR_2_COMPL_ANY_TH=16.81 PRIMER_PAIR_2_COMPL_END_TH=16.81 PRIMER_PAIR_2_PRODUCT_SIZE=175 = SEQUENCE_ID=Pick fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_START=697 SEQUENCE_FORCE_RIGHT_END=678 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=2.573940 PRIMER_LEFT_0_PENALTY=0.042322 PRIMER_RIGHT_0_PENALTY=2.531618 PRIMER_LEFT_0_SEQUENCE=ctgggggtgcgcataataac PRIMER_RIGHT_0_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_0=526,20 PRIMER_RIGHT_0=697,20 PRIMER_LEFT_0_TM=59.958 PRIMER_RIGHT_0_TM=57.468 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY_TH=2.30 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=1.8900 PRIMER_RIGHT_0_END_STABILITY=2.5700 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=172 PRIMER_PAIR_1_PENALTY=2.629283 PRIMER_LEFT_1_PENALTY=0.097666 PRIMER_RIGHT_1_PENALTY=2.531618 PRIMER_LEFT_1_SEQUENCE=ccgaagcccttcatggacta PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_1=496,20 PRIMER_RIGHT_1=697,20 PRIMER_LEFT_1_TM=60.098 PRIMER_RIGHT_1_TM=57.468 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY_TH=9.08 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=40.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=2.5900 PRIMER_RIGHT_1_END_STABILITY=2.5700 PRIMER_PAIR_1_COMPL_ANY_TH=12.74 PRIMER_PAIR_1_COMPL_END_TH=13.73 PRIMER_PAIR_1_PRODUCT_SIZE=202 PRIMER_PAIR_2_PENALTY=2.631616 PRIMER_LEFT_2_PENALTY=0.099998 PRIMER_RIGHT_2_PENALTY=2.531618 PRIMER_LEFT_2_SEQUENCE=ccgtgacttagctcgagtga PRIMER_RIGHT_2_SEQUENCE=gcttcgatgatggcttgatt PRIMER_LEFT_2=401,20 PRIMER_RIGHT_2=697,20 PRIMER_LEFT_2_TM=60.100 PRIMER_RIGHT_2_TM=57.468 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY_TH=9.34 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=3.4100 PRIMER_RIGHT_2_END_STABILITY=2.5700 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=297 = SEQUENCE_ID=Pick start fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=2.035489 PRIMER_LEFT_0_PENALTY=1.938159 PRIMER_RIGHT_0_PENALTY=0.097330 PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacctc PRIMER_RIGHT_0_SEQUENCE=aaaggtgtcccagttgacga PRIMER_LEFT_0=15,21 PRIMER_RIGHT_0=137,20 PRIMER_LEFT_0_TM=59.062 PRIMER_RIGHT_0_TM=60.097 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY_TH=0.82 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=41.92 PRIMER_RIGHT_0_HAIRPIN_TH=38.67 PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=4.2000 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=123 PRIMER_PAIR_1_PENALTY=2.036379 PRIMER_LEFT_1_PENALTY=1.938159 PRIMER_RIGHT_1_PENALTY=0.098220 PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacctc PRIMER_RIGHT_1_SEQUENCE=gtcctcgatgatggtgtcca PRIMER_LEFT_1=15,21 PRIMER_RIGHT_1=188,20 PRIMER_LEFT_1_TM=59.062 PRIMER_RIGHT_1_TM=60.098 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY_TH=0.82 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=41.92 PRIMER_RIGHT_1_HAIRPIN_TH=38.57 PRIMER_LEFT_1_END_STABILITY=3.8500 PRIMER_RIGHT_1_END_STABILITY=4.0200 PRIMER_PAIR_1_COMPL_ANY_TH=19.50 PRIMER_PAIR_1_COMPL_END_TH=18.10 PRIMER_PAIR_1_PRODUCT_SIZE=174 PRIMER_PAIR_2_PENALTY=2.036379 PRIMER_LEFT_2_PENALTY=1.938159 PRIMER_RIGHT_2_PENALTY=0.098220 PRIMER_LEFT_2_SEQUENCE=atggatgccatcaatgacctc PRIMER_RIGHT_2_SEQUENCE=tgtcctcgatgatggtgtcc PRIMER_LEFT_2=15,21 PRIMER_RIGHT_2=189,20 PRIMER_LEFT_2_TM=59.062 PRIMER_RIGHT_2_TM=60.098 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY_TH=0.82 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=41.92 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=3.8500 PRIMER_RIGHT_2_END_STABILITY=4.0200 PRIMER_PAIR_2_COMPL_ANY_TH=16.81 PRIMER_PAIR_2_COMPL_END_TH=16.81 PRIMER_PAIR_2_PRODUCT_SIZE=175 = SEQUENCE_ID=Pick start fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_START=697 PRIMER_NUM_RETURN=3 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=2.139074 PRIMER_LEFT_0_PENALTY=0.042322 PRIMER_RIGHT_0_PENALTY=2.096751 PRIMER_LEFT_0_SEQUENCE=ctgggggtgcgcataataac PRIMER_RIGHT_0_SEQUENCE=gcttcgatgatggcttgattg PRIMER_LEFT_0=526,20 PRIMER_RIGHT_0=697,21 PRIMER_LEFT_0_TM=59.958 PRIMER_RIGHT_0_TM=58.903 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY_TH=2.30 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=1.8900 PRIMER_RIGHT_0_END_STABILITY=2.6700 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=172 PRIMER_PAIR_1_PENALTY=2.194417 PRIMER_LEFT_1_PENALTY=0.097666 PRIMER_RIGHT_1_PENALTY=2.096751 PRIMER_LEFT_1_SEQUENCE=ccgaagcccttcatggacta PRIMER_RIGHT_1_SEQUENCE=gcttcgatgatggcttgattg PRIMER_LEFT_1=496,20 PRIMER_RIGHT_1=697,21 PRIMER_LEFT_1_TM=60.098 PRIMER_RIGHT_1_TM=58.903 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY_TH=9.08 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=40.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=2.5900 PRIMER_RIGHT_1_END_STABILITY=2.6700 PRIMER_PAIR_1_COMPL_ANY_TH=12.74 PRIMER_PAIR_1_COMPL_END_TH=13.73 PRIMER_PAIR_1_PRODUCT_SIZE=202 PRIMER_PAIR_2_PENALTY=2.196749 PRIMER_LEFT_2_PENALTY=0.099998 PRIMER_RIGHT_2_PENALTY=2.096751 PRIMER_LEFT_2_SEQUENCE=ccgtgacttagctcgagtga PRIMER_RIGHT_2_SEQUENCE=gcttcgatgatggcttgattg PRIMER_LEFT_2=401,20 PRIMER_RIGHT_2=697,21 PRIMER_LEFT_2_TM=60.100 PRIMER_RIGHT_2_TM=58.903 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY_TH=9.34 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=3.4100 PRIMER_RIGHT_2_END_STABILITY=2.6700 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=297 = SEQUENCE_ID=Pick end fixed left primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_END=34 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=1.757074 PRIMER_LEFT_0_PENALTY=1.659744 PRIMER_RIGHT_0_PENALTY=0.097330 PRIMER_LEFT_0_SEQUENCE=catggatgccatcaatgacct PRIMER_RIGHT_0_SEQUENCE=aaaggtgtcccagttgacga PRIMER_LEFT_0=14,21 PRIMER_RIGHT_0=137,20 PRIMER_LEFT_0_TM=59.340 PRIMER_RIGHT_0_TM=60.097 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY_TH=0.82 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=44.12 PRIMER_RIGHT_0_HAIRPIN_TH=38.67 PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=4.2000 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=124 PRIMER_PAIR_1_PENALTY=1.757964 PRIMER_LEFT_1_PENALTY=1.659744 PRIMER_RIGHT_1_PENALTY=0.098220 PRIMER_LEFT_1_SEQUENCE=catggatgccatcaatgacct PRIMER_RIGHT_1_SEQUENCE=gtcctcgatgatggtgtcca PRIMER_LEFT_1=14,21 PRIMER_RIGHT_1=188,20 PRIMER_LEFT_1_TM=59.340 PRIMER_RIGHT_1_TM=60.098 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY_TH=0.82 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=44.12 PRIMER_RIGHT_1_HAIRPIN_TH=38.57 PRIMER_LEFT_1_END_STABILITY=3.8500 PRIMER_RIGHT_1_END_STABILITY=4.0200 PRIMER_PAIR_1_COMPL_ANY_TH=19.50 PRIMER_PAIR_1_COMPL_END_TH=18.10 PRIMER_PAIR_1_PRODUCT_SIZE=175 = SEQUENCE_ID=Pick end fixed right primer SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_RIGHT_END=678 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=1.074493 PRIMER_LEFT_0_PENALTY=0.042322 PRIMER_RIGHT_0_PENALTY=1.032171 PRIMER_LEFT_0_SEQUENCE=ctgggggtgcgcataataac PRIMER_RIGHT_0_SEQUENCE=ggcttcgatgatggcttgatt PRIMER_LEFT_0=526,20 PRIMER_RIGHT_0=698,21 PRIMER_LEFT_0_TM=59.958 PRIMER_RIGHT_0_TM=59.968 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY_TH=2.30 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=31.99 PRIMER_LEFT_0_END_STABILITY=1.8900 PRIMER_RIGHT_0_END_STABILITY=2.5700 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=173 PRIMER_PAIR_1_PENALTY=1.129837 PRIMER_LEFT_1_PENALTY=0.097666 PRIMER_RIGHT_1_PENALTY=1.032171 PRIMER_LEFT_1_SEQUENCE=ccgaagcccttcatggacta PRIMER_RIGHT_1_SEQUENCE=ggcttcgatgatggcttgatt PRIMER_LEFT_1=496,20 PRIMER_RIGHT_1=698,21 PRIMER_LEFT_1_TM=60.098 PRIMER_RIGHT_1_TM=59.968 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY_TH=9.08 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=40.00 PRIMER_RIGHT_1_HAIRPIN_TH=31.99 PRIMER_LEFT_1_END_STABILITY=2.5900 PRIMER_RIGHT_1_END_STABILITY=2.5700 PRIMER_PAIR_1_COMPL_ANY_TH=23.58 PRIMER_PAIR_1_COMPL_END_TH=23.58 PRIMER_PAIR_1_PRODUCT_SIZE=203 = SEQUENCE_ID=Pick cloning primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_cloning_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_INCLUDED_REGION=15,203 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=2.951836 PRIMER_LEFT_0_PENALTY=1.938159 PRIMER_RIGHT_0_PENALTY=1.013677 PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacctc PRIMER_RIGHT_0_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_0=15,21 PRIMER_RIGHT_0=217,20 PRIMER_LEFT_0_TM=59.062 PRIMER_RIGHT_0_TM=61.014 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY_TH=0.82 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=41.92 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=3.8500 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=203 PRIMER_PAIR_1_PENALTY=3.136630 PRIMER_LEFT_1_PENALTY=2.122953 PRIMER_RIGHT_1_PENALTY=1.013677 PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_1_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_1=15,20 PRIMER_RIGHT_1=217,20 PRIMER_LEFT_1_TM=57.877 PRIMER_RIGHT_1_TM=61.014 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY_TH=0.82 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=41.92 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=3.8500 PRIMER_RIGHT_1_END_STABILITY=3.8500 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=203 = SEQUENCE_ID=Pick discriminative primers SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_discriminative_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=35,163 PRIMER_NUM_RETURN=2 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=1.738307 PRIMER_LEFT_0_PENALTY=1.426280 PRIMER_RIGHT_0_PENALTY=0.312027 PRIMER_LEFT_0_PROBLEMS= Overlaps target; PRIMER_RIGHT_0_PROBLEMS= Overlaps target; PRIMER_LEFT_0_SEQUENCE=tggatgccatcaatgacctc PRIMER_RIGHT_0_SEQUENCE=gactgagctgttcgaggaca PRIMER_LEFT_0=16,20 PRIMER_RIGHT_0=216,20 PRIMER_LEFT_0_TM=58.574 PRIMER_RIGHT_0_TM=60.312 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY_TH=0.82 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=4.0200 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=201 PRIMER_PAIR_1_PENALTY=2.250186 PRIMER_LEFT_1_PENALTY=1.938159 PRIMER_RIGHT_1_PENALTY=0.312027 PRIMER_LEFT_1_PROBLEMS= Overlaps target; PRIMER_RIGHT_1_PROBLEMS= Overlaps target; PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacctc PRIMER_RIGHT_1_SEQUENCE=gactgagctgttcgaggaca PRIMER_LEFT_1=15,21 PRIMER_RIGHT_1=216,20 PRIMER_LEFT_1_TM=59.062 PRIMER_RIGHT_1_TM=60.312 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY_TH=0.82 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=41.92 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=3.8500 PRIMER_RIGHT_1_END_STABILITY=4.0200 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=202 = SEQUENCE_ID=Pick start fixed left primer unique SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_FORCE_LEFT_START=15 PRIMER_NUM_RETURN=3 PRIMER_MIN_THREE_PRIME_DISTANCE=0 PRIMER_PICK_ANYWAY=1 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=2.035489 PRIMER_LEFT_0_PENALTY=1.938159 PRIMER_RIGHT_0_PENALTY=0.097330 PRIMER_LEFT_0_SEQUENCE=atggatgccatcaatgacctc PRIMER_RIGHT_0_SEQUENCE=aaaggtgtcccagttgacga PRIMER_LEFT_0=15,21 PRIMER_RIGHT_0=137,20 PRIMER_LEFT_0_TM=59.062 PRIMER_RIGHT_0_TM=60.097 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY_TH=0.82 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=41.92 PRIMER_RIGHT_0_HAIRPIN_TH=38.67 PRIMER_LEFT_0_END_STABILITY=3.8500 PRIMER_RIGHT_0_END_STABILITY=4.2000 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=123 PRIMER_PAIR_1_PENALTY=2.221173 PRIMER_LEFT_1_PENALTY=2.122953 PRIMER_RIGHT_1_PENALTY=0.098220 PRIMER_LEFT_1_SEQUENCE=atggatgccatcaatgacct PRIMER_RIGHT_1_SEQUENCE=gtcctcgatgatggtgtcca PRIMER_LEFT_1=15,20 PRIMER_RIGHT_1=188,20 PRIMER_LEFT_1_TM=57.877 PRIMER_RIGHT_1_TM=60.098 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY_TH=0.82 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=41.92 PRIMER_RIGHT_1_HAIRPIN_TH=38.57 PRIMER_LEFT_1_END_STABILITY=3.8500 PRIMER_RIGHT_1_END_STABILITY=4.0200 PRIMER_PAIR_1_COMPL_ANY_TH=19.50 PRIMER_PAIR_1_COMPL_END_TH=18.10 PRIMER_PAIR_1_PRODUCT_SIZE=174 PRIMER_PAIR_2_PENALTY=3.536430 PRIMER_LEFT_2_PENALTY=3.438211 PRIMER_RIGHT_2_PENALTY=0.098220 PRIMER_LEFT_2_SEQUENCE=atggatgccatcaatgacctcc PRIMER_RIGHT_2_SEQUENCE=tgtcctcgatgatggtgtcc PRIMER_LEFT_2=15,22 PRIMER_RIGHT_2=189,20 PRIMER_LEFT_2_TM=61.438 PRIMER_RIGHT_2_TM=60.098 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=41.92 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=4.3000 PRIMER_RIGHT_2_END_STABILITY=4.0200 PRIMER_PAIR_2_COMPL_ANY_TH=16.81 PRIMER_PAIR_2_COMPL_END_TH=16.81 PRIMER_PAIR_2_PRODUCT_SIZE=175 = primer3-2.3.6/test/repeat_lib40000664000077300007730000000157712231156245015164 0ustar ioanaioana>ALU--repbase Human ALU interspersed repetitive sequence - a consensus GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA >MIR--repbase mammalian-wide interspersed repeat (MIR) - a consensus GATCTAGCTAGTCGATCGTAGCTGATCTAGCTGATCGTAGCTACTACTACTAGCTAGTCTACTAGTCATCTACTATCATGCTAGCTAGTCATCTACTATCATCTACTGATGGGCGATCGTAGCGGCTAGCTAGTCGGACTGACGATATGCTAGCTACTATCTAGCTAGCTATCTACTATCATCTAGCTC >MIR2--repbase Repetitive element - a consensus TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT >L1--repbase Human L1 interspersed repetitive sequence - full length copy primer3-2.3.6/test/primer_renewed_tasks_output0000664000077300007730000003510512231156245020620 0ustar ioanaioanaPRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_EXPLAIN_FLAG=1 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_ID=pcr_primers SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, ok 3 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 3, ok 3 PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 PRIMER_PAIR_1_PENALTY=4.086374 PRIMER_LEFT_1_PENALTY=1.771733 PRIMER_RIGHT_1_PENALTY=2.314641 PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_1=1,20 PRIMER_RIGHT_1=160,18 PRIMER_LEFT_1_TM=58.228 PRIMER_RIGHT_1_TM=59.685 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=10.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=12.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=160 PRIMER_PAIR_2_PENALTY=5.046339 PRIMER_LEFT_2_PENALTY=2.731698 PRIMER_RIGHT_2_PENALTY=2.314641 PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=160,18 PRIMER_LEFT_2_TM=57.268 PRIMER_RIGHT_2_TM=59.685 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY=12.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=12.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=161 = SEQUENCE_ID=pcr_primers_and_io PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG PRIMER_LEFT_EXPLAIN=considered 281, too many Ns 210, low tm 30, high tm 29, ok 12 PRIMER_RIGHT_EXPLAIN=considered 284, too many Ns 210, low tm 7, high tm 44, ok 23 PRIMER_INTERNAL_EXPLAIN=considered 393, too many Ns 280, low tm 30, high tm 46, long poly-x seq 3, ok 34 PRIMER_PAIR_EXPLAIN=considered 5, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.334195 PRIMER_LEFT_0_PENALTY=2.101896 PRIMER_RIGHT_0_PENALTY=0.232299 PRIMER_INTERNAL_0_PENALTY=4.798289 PRIMER_LEFT_0_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_0_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_INTERNAL_0_SEQUENCE=CTTAGCGTGCGTGGGGTC PRIMER_LEFT_0=9,22 PRIMER_RIGHT_0=247,20 PRIMER_INTERNAL_0=141,18 PRIMER_LEFT_0_TM=59.898 PRIMER_RIGHT_0_TM=60.232 PRIMER_INTERNAL_0_TM=62.798 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=239 PRIMER_PAIR_1_PENALTY=2.773493 PRIMER_LEFT_1_PENALTY=2.101896 PRIMER_RIGHT_1_PENALTY=0.671597 PRIMER_INTERNAL_1_PENALTY=4.798289 PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT PRIMER_INTERNAL_1_SEQUENCE=CTTAGCGTGCGTGGGGTC PRIMER_LEFT_1=9,22 PRIMER_RIGHT_1=246,20 PRIMER_INTERNAL_1=141,18 PRIMER_LEFT_1_TM=59.898 PRIMER_RIGHT_1_TM=59.328 PRIMER_INTERNAL_1_TM=62.798 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=66.667 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=238 PRIMER_PAIR_2_PENALTY=2.846821 PRIMER_LEFT_2_PENALTY=2.101896 PRIMER_RIGHT_2_PENALTY=0.744925 PRIMER_INTERNAL_2_PENALTY=4.798289 PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA PRIMER_INTERNAL_2_SEQUENCE=CTTAGCGTGCGTGGGGTC PRIMER_LEFT_2=9,22 PRIMER_RIGHT_2=248,20 PRIMER_INTERNAL_2=141,18 PRIMER_LEFT_2_TM=59.898 PRIMER_RIGHT_2_TM=60.745 PRIMER_INTERNAL_2_TM=62.798 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=66.667 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=240 PRIMER_PAIR_3_PENALTY=3.098430 PRIMER_LEFT_3_PENALTY=2.866131 PRIMER_RIGHT_3_PENALTY=0.232299 PRIMER_INTERNAL_3_PENALTY=4.798289 PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_INTERNAL_3_SEQUENCE=CTTAGCGTGCGTGGGGTC PRIMER_LEFT_3=11,21 PRIMER_RIGHT_3=247,20 PRIMER_INTERNAL_3=141,18 PRIMER_LEFT_3_TM=58.134 PRIMER_RIGHT_3_TM=60.232 PRIMER_INTERNAL_3_TM=62.798 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=66.667 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=237 PRIMER_PAIR_4_PENALTY=3.235559 PRIMER_LEFT_4_PENALTY=3.003259 PRIMER_RIGHT_4_PENALTY=0.232299 PRIMER_INTERNAL_4_PENALTY=4.798289 PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_INTERNAL_4_SEQUENCE=CTTAGCGTGCGTGGGGTC PRIMER_LEFT_4=10,22 PRIMER_RIGHT_4=247,20 PRIMER_INTERNAL_4=141,18 PRIMER_LEFT_4_TM=58.997 PRIMER_RIGHT_4_TM=60.232 PRIMER_INTERNAL_4_TM=62.798 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=66.667 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=238 = SEQUENCE_ID=left_only PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=100,20 SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC PRIMER_LEFT_EXPLAIN=considered 1121, too many Ns 38, overlap target 23, low tm 42, high tm 866, ok 152 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.040772 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_0=72,20 PRIMER_LEFT_0_TM=59.959 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_LEFT_1_PENALTY=0.271680 PRIMER_LEFT_1_SEQUENCE=CTGCAGGTTTAGAAGGGGTG PRIMER_LEFT_1=64,20 PRIMER_LEFT_1_TM=59.728 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_LEFT_2_PENALTY=0.271680 PRIMER_LEFT_2_SEQUENCE=CACCCTGCAGGTTTAGAAGG PRIMER_LEFT_2=60,20 PRIMER_LEFT_2_TM=59.728 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_LEFT_3_PENALTY=0.312496 PRIMER_LEFT_3_SEQUENCE=AGCACACCCTGCAGGTTTAG PRIMER_LEFT_3=56,20 PRIMER_LEFT_3_TM=60.312 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=6.3000 PRIMER_LEFT_4_PENALTY=0.407941 PRIMER_LEFT_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT PRIMER_LEFT_4=65,20 PRIMER_LEFT_4_TM=59.592 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.6000 = SEQUENCE_ID=right_only PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 SEQUENCE_TARGET=90,10 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_RIGHT_EXPLAIN=considered 623, too many Ns 27, overlap target 36, GC content failed 5, low tm 230, high tm 145, high end compl 6, ok 174 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.291669 PRIMER_RIGHT_0_SEQUENCE=GTGCTTTGCGTTTTGGAAGT PRIMER_RIGHT_0=125,20 PRIMER_RIGHT_0_TM=60.292 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_RIGHT_1_PENALTY=0.727969 PRIMER_RIGHT_1_SEQUENCE=TTGGCGTTTCTATCAGGTCA PRIMER_RIGHT_1=75,20 PRIMER_RIGHT_1_TM=59.272 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_RIGHT_2_PENALTY=0.782051 PRIMER_RIGHT_2_SEQUENCE=TGCTTTGCGTTTTGGAAGTT PRIMER_RIGHT_2=124,20 PRIMER_RIGHT_2_TM=60.782 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_RIGHT_3_PENALTY=1.125062 PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA PRIMER_RIGHT_3=76,21 PRIMER_RIGHT_3_TM=60.125 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_RIGHT_4_PENALTY=1.255675 PRIMER_RIGHT_4_SEQUENCE=TGAGTTGGCGTTTCTATCAGG PRIMER_RIGHT_4=79,21 PRIMER_RIGHT_4_TM=60.256 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_RIGHT_4_END_STABILITY=8.2000 = SEQUENCE_ID=hyb_probe_only PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=0 SEQUENCE_INTERNAL_EXCLUDED_REGION=90,20 SEQUENCE_TEMPLATE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC PRIMER_INTERNAL_EXPLAIN=considered 1040, too many Ns 27, overlap excluded region 33, low tm 44, high tm 804, ok 132 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_INTERNAL_0_PENALTY=0.271680 PRIMER_INTERNAL_0_SEQUENCE=CTGCAGGTTTAGAAGGGGTG PRIMER_INTERNAL_0=64,20 PRIMER_INTERNAL_0_TM=59.728 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_INTERNAL_1_PENALTY=0.271680 PRIMER_INTERNAL_1_SEQUENCE=CACCCTGCAGGTTTAGAAGG PRIMER_INTERNAL_1=60,20 PRIMER_INTERNAL_1_TM=59.728 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_INTERNAL_2_PENALTY=0.312496 PRIMER_INTERNAL_2_SEQUENCE=AGCACACCCTGCAGGTTTAG PRIMER_INTERNAL_2=56,20 PRIMER_INTERNAL_2_TM=60.312 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_INTERNAL_3_PENALTY=0.375090 PRIMER_INTERNAL_3_SEQUENCE=CATCTGCACCCTGTAAACCC PRIMER_INTERNAL_3=1,20 PRIMER_INTERNAL_3_TM=60.375 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_INTERNAL_4_PENALTY=0.407941 PRIMER_INTERNAL_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT PRIMER_INTERNAL_4=65,20 PRIMER_INTERNAL_4_TM=59.592 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_END=0.00 = SEQUENCE_ID=left_only PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.040772 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_0=0,20 PRIMER_LEFT_0_TM=59.959 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 = SEQUENCE_ID=right_only PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.040772 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_0=0,20 PRIMER_LEFT_0_TM=59.959 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 = SEQUENCE_ID=hyb_probe_only PRIMER_INTERNAL_MIN_SIZE=20 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.040772 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_0=0,20 PRIMER_LEFT_0_TM=59.959 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 = primer3-2.3.6/test/thal_temp_output0000775000077300007730000000755512231156245016374 0ustar ioanaioana71.091765 71.091765 11.548570 11.548570 11.548570 31.012933 20.908521 23.974756 2.053418 44.555895 13.456125 0.000000 0.000000 0.000000 0.000000 -128.190694 -136.976485 -128.190694 -8.162596 -3.843450 5.910727 7.208293 7.208293 0.000000 0.000000 0.000000 0.000000 -34.030495 -35.489927 47.838549 0.000000 0.000000 0.000000 -149.430011 61.077688 0.000000 -254.658083 0.000000 -170.694738 0.000000 0.000000 21.229518 5.841287 -26.170973 0.000000 0.000000 -211.654136 0.000000 -211.654136 0.000000 0.000000 -233.286079 0.000000 0.000000 0.000000 0.000000 17.398517 15.503218 18.003397 0.000000 0.000000 -146.235483 -232.218519 0.000000 0.000000 0.000000 -164.051405 0.000000 -164.051405 0.000000 0.000000 -189.756879 0.000000 0.000000 0.000000 0.000000 -189.756879 0.000000 0.000000 0.000000 0.000000 -164.051405 0.000000 -164.051405 0.000000 0.000000 -178.782973 0.000000 0.000000 0.000000 0.000000 -146.235483 -232.218519 0.000000 0.000000 0.000000 23.974756 18.635353 -15.102937 44.555895 44.418634 7.208293 0.000000 -2.676339 0.000000 0.000000 -53.413601 -86.088279 -64.719636 47.838549 0.000000 -149.430011 0.000000 -149.430011 61.077688 0.000000 -170.694738 -254.658083 0.000000 0.000000 0.000000 21.013970 -76.120841 20.403230 0.000000 0.000000 21.229518 5.841287 -126.556128 0.000000 0.000000 -211.654136 0.000000 -211.654136 0.000000 0.000000 -233.286079 0.000000 0.000000 0.000000 0.000000 18.003397 10.998182 18.003397 0.000000 0.000000 -146.235483 -232.218519 0.000000 0.000000 0.000000 -164.051405 0.000000 -164.051405 0.000000 0.000000 -189.756879 0.000000 0.000000 0.000000 0.000000 -189.756879 0.000000 0.000000 0.000000 0.000000 -164.051405 0.000000 -164.051405 0.000000 0.000000 -178.782973 0.000000 0.000000 0.000000 0.000000 -146.235483 -232.218519 0.000000 0.000000 0.000000 23.974756 -21.764798 -83.778360 44.555895 44.418634 -3.843450 0.000000 -13.088883 0.000000 0.000000 -48.774822 -102.915345 -76.426607 47.838549 0.000000 -149.430011 0.000000 -149.430011 61.077688 0.000000 -170.694738 -254.658083 0.000000 0.000000 0.000000 10.370451 -76.120841 4.467386 0.000000 0.000000 21.229518 -1.962659 -126.556128 0.000000 0.000000 -211.654136 0.000000 -211.654136 0.000000 0.000000 -233.286079 0.000000 0.000000 0.000000 0.000000 18.003397 10.562998 18.003397 0.000000 0.000000 -146.235483 -232.218519 0.000000 0.000000 0.000000 -164.051405 0.000000 -164.051405 0.000000 0.000000 -189.756879 0.000000 0.000000 0.000000 0.000000 -189.756879 0.000000 0.000000 0.000000 0.000000 -164.051405 0.000000 -164.051405 0.000000 0.000000 -178.782973 0.000000 0.000000 0.000000 0.000000 -146.235483 -232.218519 0.000000 0.000000 0.000000 23.974756 -60.074519 -83.778360 44.555895 44.418634 -8.162596 0.000000 -89.041560 0.000000 0.000000 -66.405200 -102.915345 -88.321738 47.838549 0.000000 -149.430011 0.000000 -149.430011 61.077688 0.000000 -170.694738 -254.658083 0.000000 0.000000 0.000000 3.564153 -76.120841 -0.889075 0.000000 0.000000 21.229518 -144.596533 -126.556128 0.000000 0.000000 -211.654136 0.000000 -211.654136 0.000000 0.000000 -233.286079 0.000000 0.000000 0.000000 0.000000 0.410002 -1.764173 2.585498 0.000000 0.000000 -146.235483 -232.218519 0.000000 0.000000 0.000000 -164.051405 0.000000 -164.051405 0.000000 0.000000 -189.756879 0.000000 0.000000 0.000000 0.000000 -189.756879 0.000000 0.000000 0.000000 0.000000 -164.051405 0.000000 -164.051405 0.000000 0.000000 -178.782973 0.000000 0.000000 0.000000 0.000000 -146.235483 -232.218519 0.000000 0.000000 0.000000 28.467807 29.210876 32.832767 51.604449 58.635140 2.014351 -9.645452 -9.645452 0.000000 0.000000 30.670966 23.335163 28.271771 58.635140 58.635140 -21.988617 -21.988617 -21.988617 0.000000 0.000000 -12.463272 -11.982652 -17.567903 -17.567903 -21.102727 -15.090237 -4.272967 -21.988617 -21.988617 -21.988617 -21.988617 0.000000 0.000000 0.000000 0.000000 0.000000 14.259034 -25.497819 -18.714043 -7.775513 -25.497819 -25.497819 -14.337930 65.083895 71.425402 primer3-2.3.6/test/test_compl_error_input0000664000077300007730000000054212231156245017562 0ustar ioanaioanaP3_COMMENT=Test error in specifying PRIMER_MAX_SELF_END SEQUENCE_TEMPLATE=CTCTGCCCACATCATGGGTGAGGCCATGGAGCGCATCTATGGTGGACACCTGTGCTACGGTCCGCCCATTGAAAACGGCTTCTACTACGACATGCATCTGGAGGGCGAGGGTATCTCCACCAACGACTACGGCGCCATGGAGGGTCTG SEQUENCE_PRIMER=CTACGACATGCATCTGGAGG PRIMER_MAX_SELF_END=3 PRIMER_EXPLAIN_FLAG=1 PRIMER_TASK=pick_left_only PRIMER_PICK_ANYWAY=1 = primer3-2.3.6/test/primer_ok_regions_formatted_output0000664000077300007730000002752112231156245022171 0ustar ioanaioanaPRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 1 any right primer - OK No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 1 20 51.06 30.00 11.00 5.00 cttgattcaatgaatctcaa RIGHT PRIMER 65 18 48.54 27.78 2.00 2.00 aaaaaaacacaatccAga SEQUENCE SIZE: 100 INCLUDED REGION SIZE: 100 PRODUCT SIZE: 65, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 1,20,, 1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt >>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<< 61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg <<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.2.0 PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 2 any left primer - OK No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 1 20 51.06 30.00 11.00 5.00 cttgattcaatgaatctcaa RIGHT PRIMER 65 17 45.95 29.41 2.00 1.00 aaaaaaacacaatccAg SEQUENCE SIZE: 100 INCLUDED REGION SIZE: 100 PRODUCT SIZE: 65, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: ,,49,17 1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt >>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<< 61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg <<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.2.0 PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 3 any pair - OK No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 1 20 51.06 30.00 11.00 5.00 cttgattcaatgaatctcaa RIGHT PRIMER 65 16 43.92 25.00 2.00 0.00 aaaaaaacacaatccA SEQUENCE SIZE: 100 INCLUDED REGION SIZE: 100 PRODUCT SIZE: 65, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: ,,, 1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt >>>>>>>>>>>>>>>>>>>> <<<<<<<<<<< 61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg <<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.2.0 PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 4 constrained left and right primers - OK No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 1 20 51.06 30.00 11.00 5.00 cttgattcaatgaatctcaa RIGHT PRIMER 65 17 45.95 29.41 2.00 1.00 aaaaaaacacaatccAg SEQUENCE SIZE: 100 INCLUDED REGION SIZE: 100 PRODUCT SIZE: 65, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 1,20,49,17 1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt >>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<< 61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg <<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.2.0 PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 5 constrained left and right primers - NOT OK No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 100 INCLUDED REGION SIZE: 100 PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 5,10,60,10 1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt 61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 1 0 0 0 0 0 0 0 0 0 Right 1 0 0 0 1 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 primer3 release 2.2.0 PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 6 constrained left and right primers - OK No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 1 20 51.06 30.00 11.00 5.00 cttgattcaatgaatctcaa RIGHT PRIMER 65 17 45.95 29.41 2.00 1.00 aaaaaaacacaatccAg SEQUENCE SIZE: 100 INCLUDED REGION SIZE: 100 PRODUCT SIZE: 65, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 5,10,60,7 1,25,, 1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt >>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<< 61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg <<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.2.0 PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 7 contrained left and right primers - NOT OK No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 100 INCLUDED REGION SIZE: 100 PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 1,19,49,17 1,20,50,16 1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt 61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, not in any ok region 1, ok 0 primer3 release 2.2.0 PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 8 any left primer - NOT OK No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 100 INCLUDED REGION SIZE: 100 PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: ,,49,16 1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt 61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 1 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 primer3 release 2.2.0 PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 9 any right primer - NOT OK No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 100 INCLUDED REGION SIZE: 100 PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 2,20,, 1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt 61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 1 0 0 0 0 0 0 0 0 0 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 primer3 release 2.2.0 PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 10 any right primer, any left primer - OK No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 1 20 51.06 30.00 11.00 5.00 cttgattcaatgaatctcaa RIGHT PRIMER 65 17 45.95 29.41 2.00 1.00 aaaaaaacacaatccAg SEQUENCE SIZE: 100 INCLUDED REGION SIZE: 100 PRODUCT SIZE: 65, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 2,20,, ,,49,17 1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt >>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<< 61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg <<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 primer3 release 2.2.0 primer3-2.3.6/test/repeat_lib30000664000077300007730000001530112231156245015151 0ustar ioanaioana>ALU--repbase Human ALU interspersed repetitive sequence - a consensus GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA >MIR--repbase mammalian-wide interspersed repeat (MIR) - a consensus >MIR2--repbase Repetitive element - a consensus TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT >L1--repbase Human L1 interspersed repetitive sequence - full length copy GGCGGTGGAGCCAAGATGACCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGAC CATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACC ACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAA AA primer3-2.3.6/test/primer_task_input0000664000077300007730000000367112231156245016526 0ustar ioanaioanaPRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 P3_FILE_FLAG=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_TASK=pick_pcr_primers SEQUENCE_ID=pcr_primers SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 = SEQUENCE_ID=pcr_primers_and_io PRIMER_TASK=pick_pcr_primers_and_hyb_PROBE SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG = SEQUENCE_ID=left_only PRIMER_TASK=pick_left_only SEQUENCE_TARGET=100,20 SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC = SEQUENCE_ID=right_only PRIMER_TASK=PICK_right_only SEQUENCE_TARGET=90,10 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC = SEQUENCE_ID=hyb_probe_only PRIMER_TASK=pick_HYB_probe_ONly SEQUENCE_INTERNAL_EXCLUDED_REGION=90,20 SEQUENCE_TEMPLATE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC = SEQUENCE_ID=left_only PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG = SEQUENCE_ID=right_only PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG = SEQUENCE_ID=hyb_probe_only PRIMER_INTERNAL_MIN_SIZE=20 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG = primer3-2.3.6/test/primer_high_gc_load_set_input0000664000077300007730000000326412231156245021024 0ustar ioanaioanaSEQUENCE_ID=Load qPCR settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_TASK=pick_detection_primers = primer3-2.3.6/test/p3_3_prime_n_output0000664000077300007730000033141312231156245016662 0ustar ioanaioanaPRIMER_PRODUCT_SIZE_RANGE=100-500 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_NUM_RETURN=40 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_THREE_PRIME_DISTANCE=-1 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=40 PRIMER_RIGHT_NUM_RETURNED=40 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=40 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=7.720839 PRIMER_LEFT_1_PENALTY=7.398215 PRIMER_RIGHT_1_PENALTY=0.322623 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_1_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_1=0,22 PRIMER_RIGHT_1=186,20 PRIMER_LEFT_1_TM=54.602 PRIMER_RIGHT_1_TM=60.323 PRIMER_LEFT_1_GC_PERCENT=36.364 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.8000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=187 PRIMER_PAIR_2_PENALTY=7.730589 PRIMER_LEFT_2_PENALTY=7.407966 PRIMER_RIGHT_2_PENALTY=0.322623 PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAG PRIMER_RIGHT_2_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_2=0,21 PRIMER_RIGHT_2=186,20 PRIMER_LEFT_2_TM=53.592 PRIMER_RIGHT_2_TM=60.323 PRIMER_LEFT_2_GC_PERCENT=38.095 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.8000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=187 PRIMER_PAIR_3_PENALTY=7.734342 PRIMER_LEFT_3_PENALTY=7.018216 PRIMER_RIGHT_3_PENALTY=0.716126 PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_3_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_3=0,27 PRIMER_RIGHT_3=179,20 PRIMER_LEFT_3_TM=59.982 PRIMER_RIGHT_3_TM=59.284 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=180 PRIMER_PAIR_4_PENALTY=7.861119 PRIMER_LEFT_4_PENALTY=7.538496 PRIMER_RIGHT_4_PENALTY=0.322623 PRIMER_LEFT_4_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_4_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_4=0,25 PRIMER_RIGHT_4=186,20 PRIMER_LEFT_4_TM=57.462 PRIMER_RIGHT_4_TM=60.323 PRIMER_LEFT_4_GC_PERCENT=32.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.0000 PRIMER_RIGHT_4_END_STABILITY=8.8000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=187 PRIMER_PAIR_5_PENALTY=8.017064 PRIMER_LEFT_5_PENALTY=7.694441 PRIMER_RIGHT_5_PENALTY=0.322623 PRIMER_LEFT_5_SEQUENCE=GCCTTCATTAGAAAGTGAAA PRIMER_RIGHT_5_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_5=0,20 PRIMER_RIGHT_5=186,20 PRIMER_LEFT_5_TM=52.306 PRIMER_RIGHT_5_TM=60.323 PRIMER_LEFT_5_GC_PERCENT=35.000 PRIMER_RIGHT_5_GC_PERCENT=50.000 PRIMER_LEFT_5_SELF_ANY=5.00 PRIMER_RIGHT_5_SELF_ANY=5.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=4.00 PRIMER_LEFT_5_END_STABILITY=7.3000 PRIMER_RIGHT_5_END_STABILITY=8.8000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PAIR_5_PRODUCT_SIZE=187 PRIMER_PAIR_6_PENALTY=8.114341 PRIMER_LEFT_6_PENALTY=7.398215 PRIMER_RIGHT_6_PENALTY=0.716126 PRIMER_LEFT_6_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_6_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_6=0,22 PRIMER_RIGHT_6=179,20 PRIMER_LEFT_6_TM=54.602 PRIMER_RIGHT_6_TM=59.284 PRIMER_LEFT_6_GC_PERCENT=36.364 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_LEFT_6_SELF_ANY=5.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=3.00 PRIMER_LEFT_6_END_STABILITY=6.7000 PRIMER_RIGHT_6_END_STABILITY=6.1000 PRIMER_PAIR_6_COMPL_ANY=3.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=180 PRIMER_PAIR_7_PENALTY=8.114784 PRIMER_LEFT_7_PENALTY=7.018216 PRIMER_RIGHT_7_PENALTY=1.096569 PRIMER_LEFT_7_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_7_SEQUENCE=TTAGTACCCAGGGGATTTGC PRIMER_LEFT_7=0,27 PRIMER_RIGHT_7=187,20 PRIMER_LEFT_7_TM=59.982 PRIMER_RIGHT_7_TM=58.903 PRIMER_LEFT_7_GC_PERCENT=33.333 PRIMER_RIGHT_7_GC_PERCENT=50.000 PRIMER_LEFT_7_SELF_ANY=5.00 PRIMER_RIGHT_7_SELF_ANY=5.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_END_STABILITY=7.2000 PRIMER_RIGHT_7_END_STABILITY=8.8000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PAIR_7_PRODUCT_SIZE=188 PRIMER_PAIR_8_PENALTY=8.124092 PRIMER_LEFT_8_PENALTY=7.407966 PRIMER_RIGHT_8_PENALTY=0.716126 PRIMER_LEFT_8_SEQUENCE=GCCTTCATTAGAAAGTGAAAG PRIMER_RIGHT_8_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_8=0,21 PRIMER_RIGHT_8=179,20 PRIMER_LEFT_8_TM=53.592 PRIMER_RIGHT_8_TM=59.284 PRIMER_LEFT_8_GC_PERCENT=38.095 PRIMER_RIGHT_8_GC_PERCENT=50.000 PRIMER_LEFT_8_SELF_ANY=5.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=5.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_END_STABILITY=7.0000 PRIMER_RIGHT_8_END_STABILITY=6.1000 PRIMER_PAIR_8_COMPL_ANY=3.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PAIR_8_PRODUCT_SIZE=180 PRIMER_PAIR_9_PENALTY=8.129588 PRIMER_LEFT_9_PENALTY=7.806965 PRIMER_RIGHT_9_PENALTY=0.322623 PRIMER_LEFT_9_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAA PRIMER_RIGHT_9_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_9=0,24 PRIMER_RIGHT_9=186,20 PRIMER_LEFT_9_TM=56.193 PRIMER_RIGHT_9_TM=60.323 PRIMER_LEFT_9_GC_PERCENT=33.333 PRIMER_RIGHT_9_GC_PERCENT=50.000 PRIMER_LEFT_9_SELF_ANY=5.00 PRIMER_RIGHT_9_SELF_ANY=5.00 PRIMER_LEFT_9_SELF_END=1.00 PRIMER_RIGHT_9_SELF_END=4.00 PRIMER_LEFT_9_END_STABILITY=5.7000 PRIMER_RIGHT_9_END_STABILITY=8.8000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=187 PRIMER_PAIR_10_PENALTY=8.254622 PRIMER_LEFT_10_PENALTY=7.538496 PRIMER_RIGHT_10_PENALTY=0.716126 PRIMER_LEFT_10_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_10_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_10=0,25 PRIMER_RIGHT_10=179,20 PRIMER_LEFT_10_TM=57.462 PRIMER_RIGHT_10_TM=59.284 PRIMER_LEFT_10_GC_PERCENT=32.000 PRIMER_RIGHT_10_GC_PERCENT=50.000 PRIMER_LEFT_10_SELF_ANY=5.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=3.00 PRIMER_LEFT_10_END_STABILITY=6.0000 PRIMER_RIGHT_10_END_STABILITY=6.1000 PRIMER_PAIR_10_COMPL_ANY=3.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=180 PRIMER_PAIR_11_PENALTY=8.342439 PRIMER_LEFT_11_PENALTY=7.018216 PRIMER_RIGHT_11_PENALTY=1.324224 PRIMER_LEFT_11_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_11_SEQUENCE=AGTACCCAGGGGATTTGCA PRIMER_LEFT_11=0,27 PRIMER_RIGHT_11=185,19 PRIMER_LEFT_11_TM=59.982 PRIMER_RIGHT_11_TM=60.324 PRIMER_LEFT_11_GC_PERCENT=33.333 PRIMER_RIGHT_11_GC_PERCENT=52.632 PRIMER_LEFT_11_SELF_ANY=5.00 PRIMER_RIGHT_11_SELF_ANY=5.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=4.00 PRIMER_LEFT_11_END_STABILITY=7.2000 PRIMER_RIGHT_11_END_STABILITY=8.8000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PAIR_11_PRODUCT_SIZE=186 PRIMER_PAIR_12_PENALTY=8.408934 PRIMER_LEFT_12_PENALTY=8.086311 PRIMER_RIGHT_12_PENALTY=0.322623 PRIMER_LEFT_12_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_12_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_12=30,27 PRIMER_RIGHT_12=186,20 PRIMER_LEFT_12_TM=58.914 PRIMER_RIGHT_12_TM=60.323 PRIMER_LEFT_12_GC_PERCENT=29.630 PRIMER_RIGHT_12_GC_PERCENT=50.000 PRIMER_LEFT_12_SELF_ANY=6.00 PRIMER_RIGHT_12_SELF_ANY=5.00 PRIMER_LEFT_12_SELF_END=6.00 PRIMER_RIGHT_12_SELF_END=4.00 PRIMER_LEFT_12_END_STABILITY=6.4000 PRIMER_RIGHT_12_END_STABILITY=8.8000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PAIR_12_PRODUCT_SIZE=157 PRIMER_PAIR_13_PENALTY=8.410567 PRIMER_LEFT_13_PENALTY=7.694441 PRIMER_RIGHT_13_PENALTY=0.716126 PRIMER_LEFT_13_SEQUENCE=GCCTTCATTAGAAAGTGAAA PRIMER_RIGHT_13_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_13=0,20 PRIMER_RIGHT_13=179,20 PRIMER_LEFT_13_TM=52.306 PRIMER_RIGHT_13_TM=59.284 PRIMER_LEFT_13_GC_PERCENT=35.000 PRIMER_RIGHT_13_GC_PERCENT=50.000 PRIMER_LEFT_13_SELF_ANY=5.00 PRIMER_RIGHT_13_SELF_ANY=6.00 PRIMER_LEFT_13_SELF_END=3.00 PRIMER_RIGHT_13_SELF_END=3.00 PRIMER_LEFT_13_END_STABILITY=7.3000 PRIMER_RIGHT_13_END_STABILITY=6.1000 PRIMER_PAIR_13_COMPL_ANY=3.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PAIR_13_PRODUCT_SIZE=180 PRIMER_PAIR_14_PENALTY=8.494784 PRIMER_LEFT_14_PENALTY=7.398215 PRIMER_RIGHT_14_PENALTY=1.096569 PRIMER_LEFT_14_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_14_SEQUENCE=TTAGTACCCAGGGGATTTGC PRIMER_LEFT_14=0,22 PRIMER_RIGHT_14=187,20 PRIMER_LEFT_14_TM=54.602 PRIMER_RIGHT_14_TM=58.903 PRIMER_LEFT_14_GC_PERCENT=36.364 PRIMER_RIGHT_14_GC_PERCENT=50.000 PRIMER_LEFT_14_SELF_ANY=5.00 PRIMER_RIGHT_14_SELF_ANY=5.00 PRIMER_LEFT_14_SELF_END=3.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=8.8000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PAIR_14_PRODUCT_SIZE=188 PRIMER_PAIR_15_PENALTY=8.504534 PRIMER_LEFT_15_PENALTY=7.407966 PRIMER_RIGHT_15_PENALTY=1.096569 PRIMER_LEFT_15_SEQUENCE=GCCTTCATTAGAAAGTGAAAG PRIMER_RIGHT_15_SEQUENCE=TTAGTACCCAGGGGATTTGC PRIMER_LEFT_15=0,21 PRIMER_RIGHT_15=187,20 PRIMER_LEFT_15_TM=53.592 PRIMER_RIGHT_15_TM=58.903 PRIMER_LEFT_15_GC_PERCENT=38.095 PRIMER_RIGHT_15_GC_PERCENT=50.000 PRIMER_LEFT_15_SELF_ANY=5.00 PRIMER_RIGHT_15_SELF_ANY=5.00 PRIMER_LEFT_15_SELF_END=5.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_END_STABILITY=7.0000 PRIMER_RIGHT_15_END_STABILITY=8.8000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PAIR_15_PRODUCT_SIZE=188 PRIMER_PAIR_16_PENALTY=8.523091 PRIMER_LEFT_16_PENALTY=7.806965 PRIMER_RIGHT_16_PENALTY=0.716126 PRIMER_LEFT_16_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAA PRIMER_RIGHT_16_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_16=0,24 PRIMER_RIGHT_16=179,20 PRIMER_LEFT_16_TM=56.193 PRIMER_RIGHT_16_TM=59.284 PRIMER_LEFT_16_GC_PERCENT=33.333 PRIMER_RIGHT_16_GC_PERCENT=50.000 PRIMER_LEFT_16_SELF_ANY=5.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=1.00 PRIMER_RIGHT_16_SELF_END=3.00 PRIMER_LEFT_16_END_STABILITY=5.7000 PRIMER_RIGHT_16_END_STABILITY=6.1000 PRIMER_PAIR_16_COMPL_ANY=3.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PAIR_16_PRODUCT_SIZE=180 PRIMER_PAIR_17_PENALTY=8.523324 PRIMER_LEFT_17_PENALTY=8.200700 PRIMER_RIGHT_17_PENALTY=0.322623 PRIMER_LEFT_17_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTA PRIMER_RIGHT_17_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_17=0,23 PRIMER_RIGHT_17=186,20 PRIMER_LEFT_17_TM=54.799 PRIMER_RIGHT_17_TM=60.323 PRIMER_LEFT_17_GC_PERCENT=34.783 PRIMER_RIGHT_17_GC_PERCENT=50.000 PRIMER_LEFT_17_SELF_ANY=5.00 PRIMER_RIGHT_17_SELF_ANY=5.00 PRIMER_LEFT_17_SELF_END=2.00 PRIMER_RIGHT_17_SELF_END=4.00 PRIMER_LEFT_17_END_STABILITY=5.7000 PRIMER_RIGHT_17_END_STABILITY=8.8000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PAIR_17_PRODUCT_SIZE=187 PRIMER_PAIR_18_PENALTY=8.534546 PRIMER_LEFT_18_PENALTY=8.211922 PRIMER_RIGHT_18_PENALTY=0.322623 PRIMER_LEFT_18_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAAT PRIMER_RIGHT_18_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_18=0,26 PRIMER_RIGHT_18=186,20 PRIMER_LEFT_18_TM=57.788 PRIMER_RIGHT_18_TM=60.323 PRIMER_LEFT_18_GC_PERCENT=30.769 PRIMER_RIGHT_18_GC_PERCENT=50.000 PRIMER_LEFT_18_SELF_ANY=5.00 PRIMER_RIGHT_18_SELF_ANY=5.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=4.00 PRIMER_LEFT_18_END_STABILITY=6.2000 PRIMER_RIGHT_18_END_STABILITY=8.8000 PRIMER_PAIR_18_COMPL_ANY=4.00 PRIMER_PAIR_18_COMPL_END=3.00 PRIMER_PAIR_18_PRODUCT_SIZE=187 PRIMER_PAIR_19_PENALTY=8.633610 PRIMER_LEFT_19_PENALTY=7.018216 PRIMER_RIGHT_19_PENALTY=1.615394 PRIMER_LEFT_19_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_19_SEQUENCE=CAGGGGATTTGCAAGAGACTT PRIMER_LEFT_19=0,27 PRIMER_RIGHT_19=179,21 PRIMER_LEFT_19_TM=59.982 PRIMER_RIGHT_19_TM=60.615 PRIMER_LEFT_19_GC_PERCENT=33.333 PRIMER_RIGHT_19_GC_PERCENT=47.619 PRIMER_LEFT_19_SELF_ANY=5.00 PRIMER_RIGHT_19_SELF_ANY=6.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_END_STABILITY=7.2000 PRIMER_RIGHT_19_END_STABILITY=6.4000 PRIMER_PAIR_19_COMPL_ANY=4.00 PRIMER_PAIR_19_COMPL_END=1.00 PRIMER_PAIR_19_PRODUCT_SIZE=180 PRIMER_PAIR_20_PENALTY=8.633669 PRIMER_LEFT_20_PENALTY=7.018216 PRIMER_RIGHT_20_PENALTY=1.615453 PRIMER_LEFT_20_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_20_SEQUENCE=GTACCCAGGGGATTTGCAAG PRIMER_LEFT_20=0,27 PRIMER_RIGHT_20=184,20 PRIMER_LEFT_20_TM=59.982 PRIMER_RIGHT_20_TM=61.615 PRIMER_LEFT_20_GC_PERCENT=33.333 PRIMER_RIGHT_20_GC_PERCENT=55.000 PRIMER_LEFT_20_SELF_ANY=5.00 PRIMER_RIGHT_20_SELF_ANY=6.00 PRIMER_LEFT_20_SELF_END=0.00 PRIMER_RIGHT_20_SELF_END=4.00 PRIMER_LEFT_20_END_STABILITY=7.2000 PRIMER_RIGHT_20_END_STABILITY=8.5000 PRIMER_PAIR_20_COMPL_ANY=4.00 PRIMER_PAIR_20_COMPL_END=0.00 PRIMER_PAIR_20_PRODUCT_SIZE=185 PRIMER_PAIR_21_PENALTY=8.633669 PRIMER_LEFT_21_PENALTY=7.018216 PRIMER_RIGHT_21_PENALTY=1.615453 PRIMER_LEFT_21_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_21_SEQUENCE=AGTACCCAGGGGATTTGCAA PRIMER_LEFT_21=0,27 PRIMER_RIGHT_21=185,20 PRIMER_LEFT_21_TM=59.982 PRIMER_RIGHT_21_TM=61.615 PRIMER_LEFT_21_GC_PERCENT=33.333 PRIMER_RIGHT_21_GC_PERCENT=50.000 PRIMER_LEFT_21_SELF_ANY=5.00 PRIMER_RIGHT_21_SELF_ANY=6.00 PRIMER_LEFT_21_SELF_END=0.00 PRIMER_RIGHT_21_SELF_END=6.00 PRIMER_LEFT_21_END_STABILITY=7.2000 PRIMER_RIGHT_21_END_STABILITY=8.8000 PRIMER_PAIR_21_COMPL_ANY=4.00 PRIMER_PAIR_21_COMPL_END=1.00 PRIMER_PAIR_21_PRODUCT_SIZE=186 PRIMER_PAIR_22_PENALTY=8.635064 PRIMER_LEFT_22_PENALTY=7.538496 PRIMER_RIGHT_22_PENALTY=1.096569 PRIMER_LEFT_22_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_22_SEQUENCE=TTAGTACCCAGGGGATTTGC PRIMER_LEFT_22=0,25 PRIMER_RIGHT_22=187,20 PRIMER_LEFT_22_TM=57.462 PRIMER_RIGHT_22_TM=58.903 PRIMER_LEFT_22_GC_PERCENT=32.000 PRIMER_RIGHT_22_GC_PERCENT=50.000 PRIMER_LEFT_22_SELF_ANY=5.00 PRIMER_RIGHT_22_SELF_ANY=5.00 PRIMER_LEFT_22_SELF_END=0.00 PRIMER_RIGHT_22_SELF_END=2.00 PRIMER_LEFT_22_END_STABILITY=6.0000 PRIMER_RIGHT_22_END_STABILITY=8.8000 PRIMER_PAIR_22_COMPL_ANY=3.00 PRIMER_PAIR_22_COMPL_END=3.00 PRIMER_PAIR_22_PRODUCT_SIZE=188 PRIMER_PAIR_23_PENALTY=8.722439 PRIMER_LEFT_23_PENALTY=7.398215 PRIMER_RIGHT_23_PENALTY=1.324224 PRIMER_LEFT_23_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_23_SEQUENCE=AGTACCCAGGGGATTTGCA PRIMER_LEFT_23=0,22 PRIMER_RIGHT_23=185,19 PRIMER_LEFT_23_TM=54.602 PRIMER_RIGHT_23_TM=60.324 PRIMER_LEFT_23_GC_PERCENT=36.364 PRIMER_RIGHT_23_GC_PERCENT=52.632 PRIMER_LEFT_23_SELF_ANY=5.00 PRIMER_RIGHT_23_SELF_ANY=5.00 PRIMER_LEFT_23_SELF_END=3.00 PRIMER_RIGHT_23_SELF_END=4.00 PRIMER_LEFT_23_END_STABILITY=6.7000 PRIMER_RIGHT_23_END_STABILITY=8.8000 PRIMER_PAIR_23_COMPL_ANY=3.00 PRIMER_PAIR_23_COMPL_END=3.00 PRIMER_PAIR_23_PRODUCT_SIZE=186 PRIMER_PAIR_24_PENALTY=8.725927 PRIMER_LEFT_24_PENALTY=7.018216 PRIMER_RIGHT_24_PENALTY=1.707711 PRIMER_LEFT_24_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_24_SEQUENCE=GTACCCAGGGGATTTGCAA PRIMER_LEFT_24=0,27 PRIMER_RIGHT_24=184,19 PRIMER_LEFT_24_TM=59.982 PRIMER_RIGHT_24_TM=60.708 PRIMER_LEFT_24_GC_PERCENT=33.333 PRIMER_RIGHT_24_GC_PERCENT=52.632 PRIMER_LEFT_24_SELF_ANY=5.00 PRIMER_RIGHT_24_SELF_ANY=6.00 PRIMER_LEFT_24_SELF_END=0.00 PRIMER_RIGHT_24_SELF_END=6.00 PRIMER_LEFT_24_END_STABILITY=7.2000 PRIMER_RIGHT_24_END_STABILITY=8.8000 PRIMER_PAIR_24_COMPL_ANY=4.00 PRIMER_PAIR_24_COMPL_END=1.00 PRIMER_PAIR_24_PRODUCT_SIZE=185 PRIMER_PAIR_25_PENALTY=8.732189 PRIMER_LEFT_25_PENALTY=7.407966 PRIMER_RIGHT_25_PENALTY=1.324224 PRIMER_LEFT_25_SEQUENCE=GCCTTCATTAGAAAGTGAAAG PRIMER_RIGHT_25_SEQUENCE=AGTACCCAGGGGATTTGCA PRIMER_LEFT_25=0,21 PRIMER_RIGHT_25=185,19 PRIMER_LEFT_25_TM=53.592 PRIMER_RIGHT_25_TM=60.324 PRIMER_LEFT_25_GC_PERCENT=38.095 PRIMER_RIGHT_25_GC_PERCENT=52.632 PRIMER_LEFT_25_SELF_ANY=5.00 PRIMER_RIGHT_25_SELF_ANY=5.00 PRIMER_LEFT_25_SELF_END=5.00 PRIMER_RIGHT_25_SELF_END=4.00 PRIMER_LEFT_25_END_STABILITY=7.0000 PRIMER_RIGHT_25_END_STABILITY=8.8000 PRIMER_PAIR_25_COMPL_ANY=3.00 PRIMER_PAIR_25_COMPL_END=2.00 PRIMER_PAIR_25_PRODUCT_SIZE=186 PRIMER_PAIR_26_PENALTY=8.777838 PRIMER_LEFT_26_PENALTY=7.018216 PRIMER_RIGHT_26_PENALTY=1.759622 PRIMER_LEFT_26_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_26_SEQUENCE=GGGGATTTGCAAGAGACTTT PRIMER_LEFT_26=0,27 PRIMER_RIGHT_26=177,20 PRIMER_LEFT_26_TM=59.982 PRIMER_RIGHT_26_TM=58.240 PRIMER_LEFT_26_GC_PERCENT=33.333 PRIMER_RIGHT_26_GC_PERCENT=45.000 PRIMER_LEFT_26_SELF_ANY=5.00 PRIMER_RIGHT_26_SELF_ANY=6.00 PRIMER_LEFT_26_SELF_END=0.00 PRIMER_RIGHT_26_SELF_END=3.00 PRIMER_LEFT_26_END_STABILITY=7.2000 PRIMER_RIGHT_26_END_STABILITY=6.7000 PRIMER_PAIR_26_COMPL_ANY=5.00 PRIMER_PAIR_26_COMPL_END=2.00 PRIMER_PAIR_26_PRODUCT_SIZE=178 PRIMER_PAIR_27_PENALTY=8.791009 PRIMER_LEFT_27_PENALTY=7.694441 PRIMER_RIGHT_27_PENALTY=1.096569 PRIMER_LEFT_27_SEQUENCE=GCCTTCATTAGAAAGTGAAA PRIMER_RIGHT_27_SEQUENCE=TTAGTACCCAGGGGATTTGC PRIMER_LEFT_27=0,20 PRIMER_RIGHT_27=187,20 PRIMER_LEFT_27_TM=52.306 PRIMER_RIGHT_27_TM=58.903 PRIMER_LEFT_27_GC_PERCENT=35.000 PRIMER_RIGHT_27_GC_PERCENT=50.000 PRIMER_LEFT_27_SELF_ANY=5.00 PRIMER_RIGHT_27_SELF_ANY=5.00 PRIMER_LEFT_27_SELF_END=3.00 PRIMER_RIGHT_27_SELF_END=2.00 PRIMER_LEFT_27_END_STABILITY=7.3000 PRIMER_RIGHT_27_END_STABILITY=8.8000 PRIMER_PAIR_27_COMPL_ANY=3.00 PRIMER_PAIR_27_COMPL_END=2.00 PRIMER_PAIR_27_PRODUCT_SIZE=188 PRIMER_PAIR_28_PENALTY=8.791406 PRIMER_LEFT_28_PENALTY=7.018216 PRIMER_RIGHT_28_PENALTY=1.773191 PRIMER_LEFT_28_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_28_SEQUENCE=AGGGGATTTGCAAGAGACTTT PRIMER_LEFT_28=0,27 PRIMER_RIGHT_28=178,21 PRIMER_LEFT_28_TM=59.982 PRIMER_RIGHT_28_TM=59.227 PRIMER_LEFT_28_GC_PERCENT=33.333 PRIMER_RIGHT_28_GC_PERCENT=42.857 PRIMER_LEFT_28_SELF_ANY=5.00 PRIMER_RIGHT_28_SELF_ANY=6.00 PRIMER_LEFT_28_SELF_END=0.00 PRIMER_RIGHT_28_SELF_END=3.00 PRIMER_LEFT_28_END_STABILITY=7.2000 PRIMER_RIGHT_28_END_STABILITY=6.7000 PRIMER_PAIR_28_COMPL_ANY=5.00 PRIMER_PAIR_28_COMPL_END=2.00 PRIMER_PAIR_28_PRODUCT_SIZE=179 PRIMER_PAIR_29_PENALTY=8.802437 PRIMER_LEFT_29_PENALTY=8.086311 PRIMER_RIGHT_29_PENALTY=0.716126 PRIMER_LEFT_29_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_29_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_29=30,27 PRIMER_RIGHT_29=179,20 PRIMER_LEFT_29_TM=58.914 PRIMER_RIGHT_29_TM=59.284 PRIMER_LEFT_29_GC_PERCENT=29.630 PRIMER_RIGHT_29_GC_PERCENT=50.000 PRIMER_LEFT_29_SELF_ANY=6.00 PRIMER_RIGHT_29_SELF_ANY=6.00 PRIMER_LEFT_29_SELF_END=6.00 PRIMER_RIGHT_29_SELF_END=3.00 PRIMER_LEFT_29_END_STABILITY=6.4000 PRIMER_RIGHT_29_END_STABILITY=6.1000 PRIMER_PAIR_29_COMPL_ANY=5.00 PRIMER_PAIR_29_COMPL_END=3.00 PRIMER_PAIR_29_PRODUCT_SIZE=150 PRIMER_PAIR_30_PENALTY=8.855777 PRIMER_LEFT_30_PENALTY=7.018216 PRIMER_RIGHT_30_PENALTY=1.837562 PRIMER_LEFT_30_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_30_SEQUENCE=TACCCAGGGGATTTGCAAG PRIMER_LEFT_30=0,27 PRIMER_RIGHT_30=183,19 PRIMER_LEFT_30_TM=59.982 PRIMER_RIGHT_30_TM=60.838 PRIMER_LEFT_30_GC_PERCENT=33.333 PRIMER_RIGHT_30_GC_PERCENT=52.632 PRIMER_LEFT_30_SELF_ANY=5.00 PRIMER_RIGHT_30_SELF_ANY=6.00 PRIMER_LEFT_30_SELF_END=0.00 PRIMER_RIGHT_30_SELF_END=4.00 PRIMER_LEFT_30_END_STABILITY=7.2000 PRIMER_RIGHT_30_END_STABILITY=8.5000 PRIMER_PAIR_30_COMPL_ANY=4.00 PRIMER_PAIR_30_COMPL_END=0.00 PRIMER_PAIR_30_PRODUCT_SIZE=184 PRIMER_PAIR_31_PENALTY=8.862719 PRIMER_LEFT_31_PENALTY=7.538496 PRIMER_RIGHT_31_PENALTY=1.324224 PRIMER_LEFT_31_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_31_SEQUENCE=AGTACCCAGGGGATTTGCA PRIMER_LEFT_31=0,25 PRIMER_RIGHT_31=185,19 PRIMER_LEFT_31_TM=57.462 PRIMER_RIGHT_31_TM=60.324 PRIMER_LEFT_31_GC_PERCENT=32.000 PRIMER_RIGHT_31_GC_PERCENT=52.632 PRIMER_LEFT_31_SELF_ANY=5.00 PRIMER_RIGHT_31_SELF_ANY=5.00 PRIMER_LEFT_31_SELF_END=0.00 PRIMER_RIGHT_31_SELF_END=4.00 PRIMER_LEFT_31_END_STABILITY=6.0000 PRIMER_RIGHT_31_END_STABILITY=8.8000 PRIMER_PAIR_31_COMPL_ANY=3.00 PRIMER_PAIR_31_COMPL_END=2.00 PRIMER_PAIR_31_PRODUCT_SIZE=186 PRIMER_PAIR_32_PENALTY=8.903534 PRIMER_LEFT_32_PENALTY=7.806965 PRIMER_RIGHT_32_PENALTY=1.096569 PRIMER_LEFT_32_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAA PRIMER_RIGHT_32_SEQUENCE=TTAGTACCCAGGGGATTTGC PRIMER_LEFT_32=0,24 PRIMER_RIGHT_32=187,20 PRIMER_LEFT_32_TM=56.193 PRIMER_RIGHT_32_TM=58.903 PRIMER_LEFT_32_GC_PERCENT=33.333 PRIMER_RIGHT_32_GC_PERCENT=50.000 PRIMER_LEFT_32_SELF_ANY=5.00 PRIMER_RIGHT_32_SELF_ANY=5.00 PRIMER_LEFT_32_SELF_END=1.00 PRIMER_RIGHT_32_SELF_END=2.00 PRIMER_LEFT_32_END_STABILITY=5.7000 PRIMER_RIGHT_32_END_STABILITY=8.8000 PRIMER_PAIR_32_COMPL_ANY=3.00 PRIMER_PAIR_32_COMPL_END=2.00 PRIMER_PAIR_32_PRODUCT_SIZE=188 PRIMER_PAIR_33_PENALTY=8.916826 PRIMER_LEFT_33_PENALTY=8.200700 PRIMER_RIGHT_33_PENALTY=0.716126 PRIMER_LEFT_33_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTA PRIMER_RIGHT_33_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_33=0,23 PRIMER_RIGHT_33=179,20 PRIMER_LEFT_33_TM=54.799 PRIMER_RIGHT_33_TM=59.284 PRIMER_LEFT_33_GC_PERCENT=34.783 PRIMER_RIGHT_33_GC_PERCENT=50.000 PRIMER_LEFT_33_SELF_ANY=5.00 PRIMER_RIGHT_33_SELF_ANY=6.00 PRIMER_LEFT_33_SELF_END=2.00 PRIMER_RIGHT_33_SELF_END=3.00 PRIMER_LEFT_33_END_STABILITY=5.7000 PRIMER_RIGHT_33_END_STABILITY=6.1000 PRIMER_PAIR_33_COMPL_ANY=3.00 PRIMER_PAIR_33_COMPL_END=2.00 PRIMER_PAIR_33_PRODUCT_SIZE=180 PRIMER_PAIR_34_PENALTY=8.919992 PRIMER_LEFT_34_PENALTY=8.597369 PRIMER_RIGHT_34_PENALTY=0.322623 PRIMER_LEFT_34_SEQUENCE=CCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_34_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_34=31,26 PRIMER_RIGHT_34=186,20 PRIMER_LEFT_34_TM=57.403 PRIMER_RIGHT_34_TM=60.323 PRIMER_LEFT_34_GC_PERCENT=30.769 PRIMER_RIGHT_34_GC_PERCENT=50.000 PRIMER_LEFT_34_SELF_ANY=6.00 PRIMER_RIGHT_34_SELF_ANY=5.00 PRIMER_LEFT_34_SELF_END=6.00 PRIMER_RIGHT_34_SELF_END=4.00 PRIMER_LEFT_34_END_STABILITY=6.4000 PRIMER_RIGHT_34_END_STABILITY=8.8000 PRIMER_PAIR_34_COMPL_ANY=5.00 PRIMER_PAIR_34_COMPL_END=3.00 PRIMER_PAIR_34_PRODUCT_SIZE=156 PRIMER_PAIR_35_PENALTY=8.928048 PRIMER_LEFT_35_PENALTY=8.211922 PRIMER_RIGHT_35_PENALTY=0.716126 PRIMER_LEFT_35_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAAT PRIMER_RIGHT_35_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_35=0,26 PRIMER_RIGHT_35=179,20 PRIMER_LEFT_35_TM=57.788 PRIMER_RIGHT_35_TM=59.284 PRIMER_LEFT_35_GC_PERCENT=30.769 PRIMER_RIGHT_35_GC_PERCENT=50.000 PRIMER_LEFT_35_SELF_ANY=5.00 PRIMER_RIGHT_35_SELF_ANY=6.00 PRIMER_LEFT_35_SELF_END=2.00 PRIMER_RIGHT_35_SELF_END=3.00 PRIMER_LEFT_35_END_STABILITY=6.2000 PRIMER_RIGHT_35_END_STABILITY=6.1000 PRIMER_PAIR_35_COMPL_ANY=4.00 PRIMER_PAIR_35_COMPL_END=1.00 PRIMER_PAIR_35_PRODUCT_SIZE=180 PRIMER_PAIR_36_PENALTY=8.938856 PRIMER_LEFT_36_PENALTY=7.018216 PRIMER_RIGHT_36_PENALTY=1.920640 PRIMER_LEFT_36_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_36_SEQUENCE=CCAGGGGATTTGCAAGAGAC PRIMER_LEFT_36=0,27 PRIMER_RIGHT_36=180,20 PRIMER_LEFT_36_TM=59.982 PRIMER_RIGHT_36_TM=61.921 PRIMER_LEFT_36_GC_PERCENT=33.333 PRIMER_RIGHT_36_GC_PERCENT=55.000 PRIMER_LEFT_36_SELF_ANY=5.00 PRIMER_RIGHT_36_SELF_ANY=6.00 PRIMER_LEFT_36_SELF_END=0.00 PRIMER_RIGHT_36_SELF_END=2.00 PRIMER_LEFT_36_END_STABILITY=7.2000 PRIMER_RIGHT_36_END_STABILITY=6.1000 PRIMER_PAIR_36_COMPL_ANY=4.00 PRIMER_PAIR_36_COMPL_END=1.00 PRIMER_PAIR_36_PRODUCT_SIZE=181 PRIMER_PAIR_37_PENALTY=8.990513 PRIMER_LEFT_37_PENALTY=7.018216 PRIMER_RIGHT_37_PENALTY=1.972297 PRIMER_LEFT_37_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_37_SEQUENCE=GGGGATTTGCAAGAGACTTTG PRIMER_LEFT_37=0,27 PRIMER_RIGHT_37=177,21 PRIMER_LEFT_37_TM=59.982 PRIMER_RIGHT_37_TM=60.972 PRIMER_LEFT_37_GC_PERCENT=33.333 PRIMER_RIGHT_37_GC_PERCENT=47.619 PRIMER_LEFT_37_SELF_ANY=5.00 PRIMER_RIGHT_37_SELF_ANY=6.00 PRIMER_LEFT_37_SELF_END=0.00 PRIMER_RIGHT_37_SELF_END=5.00 PRIMER_LEFT_37_END_STABILITY=7.2000 PRIMER_RIGHT_37_END_STABILITY=7.3000 PRIMER_PAIR_37_COMPL_ANY=5.00 PRIMER_PAIR_37_COMPL_END=1.00 PRIMER_PAIR_37_PRODUCT_SIZE=178 PRIMER_PAIR_38_PENALTY=9.013610 PRIMER_LEFT_38_PENALTY=7.398215 PRIMER_RIGHT_38_PENALTY=1.615394 PRIMER_LEFT_38_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_38_SEQUENCE=CAGGGGATTTGCAAGAGACTT PRIMER_LEFT_38=0,22 PRIMER_RIGHT_38=179,21 PRIMER_LEFT_38_TM=54.602 PRIMER_RIGHT_38_TM=60.615 PRIMER_LEFT_38_GC_PERCENT=36.364 PRIMER_RIGHT_38_GC_PERCENT=47.619 PRIMER_LEFT_38_SELF_ANY=5.00 PRIMER_RIGHT_38_SELF_ANY=6.00 PRIMER_LEFT_38_SELF_END=3.00 PRIMER_RIGHT_38_SELF_END=3.00 PRIMER_LEFT_38_END_STABILITY=6.7000 PRIMER_RIGHT_38_END_STABILITY=6.4000 PRIMER_PAIR_38_COMPL_ANY=4.00 PRIMER_PAIR_38_COMPL_END=4.00 PRIMER_PAIR_38_PRODUCT_SIZE=180 PRIMER_PAIR_39_PENALTY=9.013669 PRIMER_LEFT_39_PENALTY=7.398215 PRIMER_RIGHT_39_PENALTY=1.615453 PRIMER_LEFT_39_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_39_SEQUENCE=GTACCCAGGGGATTTGCAAG PRIMER_LEFT_39=0,22 PRIMER_RIGHT_39=184,20 PRIMER_LEFT_39_TM=54.602 PRIMER_RIGHT_39_TM=61.615 PRIMER_LEFT_39_GC_PERCENT=36.364 PRIMER_RIGHT_39_GC_PERCENT=55.000 PRIMER_LEFT_39_SELF_ANY=5.00 PRIMER_RIGHT_39_SELF_ANY=6.00 PRIMER_LEFT_39_SELF_END=3.00 PRIMER_RIGHT_39_SELF_END=4.00 PRIMER_LEFT_39_END_STABILITY=6.7000 PRIMER_RIGHT_39_END_STABILITY=8.5000 PRIMER_PAIR_39_COMPL_ANY=3.00 PRIMER_PAIR_39_COMPL_END=1.00 PRIMER_PAIR_39_PRODUCT_SIZE=185 = PRIMER_MIN_THREE_PRIME_DISTANCE=0 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=40 PRIMER_RIGHT_NUM_RETURNED=40 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=40 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=8.114341 PRIMER_LEFT_1_PENALTY=7.398215 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_1=0,22 PRIMER_RIGHT_1=179,20 PRIMER_LEFT_1_TM=54.602 PRIMER_RIGHT_1_TM=59.284 PRIMER_LEFT_1_GC_PERCENT=36.364 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=180 PRIMER_PAIR_2_PENALTY=8.504534 PRIMER_LEFT_2_PENALTY=7.407966 PRIMER_RIGHT_2_PENALTY=1.096569 PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAG PRIMER_RIGHT_2_SEQUENCE=TTAGTACCCAGGGGATTTGC PRIMER_LEFT_2=0,21 PRIMER_RIGHT_2=187,20 PRIMER_LEFT_2_TM=53.592 PRIMER_RIGHT_2_TM=58.903 PRIMER_LEFT_2_GC_PERCENT=38.095 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.8000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=188 PRIMER_PAIR_3_PENALTY=8.862719 PRIMER_LEFT_3_PENALTY=7.538496 PRIMER_RIGHT_3_PENALTY=1.324224 PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_3_SEQUENCE=AGTACCCAGGGGATTTGCA PRIMER_LEFT_3=0,25 PRIMER_RIGHT_3=185,19 PRIMER_LEFT_3_TM=57.462 PRIMER_RIGHT_3_TM=60.324 PRIMER_LEFT_3_GC_PERCENT=32.000 PRIMER_RIGHT_3_GC_PERCENT=52.632 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.0000 PRIMER_RIGHT_3_END_STABILITY=8.8000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=186 PRIMER_PAIR_4_PENALTY=9.309835 PRIMER_LEFT_4_PENALTY=7.694441 PRIMER_RIGHT_4_PENALTY=1.615394 PRIMER_LEFT_4_SEQUENCE=GCCTTCATTAGAAAGTGAAA PRIMER_RIGHT_4_SEQUENCE=CAGGGGATTTGCAAGAGACTT PRIMER_LEFT_4=0,20 PRIMER_RIGHT_4=179,21 PRIMER_LEFT_4_TM=52.306 PRIMER_RIGHT_4_TM=60.615 PRIMER_LEFT_4_GC_PERCENT=35.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=180 PRIMER_PAIR_5_PENALTY=9.422419 PRIMER_LEFT_5_PENALTY=7.806965 PRIMER_RIGHT_5_PENALTY=1.615453 PRIMER_LEFT_5_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAA PRIMER_RIGHT_5_SEQUENCE=GTACCCAGGGGATTTGCAAG PRIMER_LEFT_5=0,24 PRIMER_RIGHT_5=184,20 PRIMER_LEFT_5_TM=56.193 PRIMER_RIGHT_5_TM=61.615 PRIMER_LEFT_5_GC_PERCENT=33.333 PRIMER_RIGHT_5_GC_PERCENT=55.000 PRIMER_LEFT_5_SELF_ANY=5.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=4.00 PRIMER_LEFT_5_END_STABILITY=5.7000 PRIMER_RIGHT_5_END_STABILITY=8.5000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PAIR_5_PRODUCT_SIZE=185 PRIMER_PAIR_6_PENALTY=9.701764 PRIMER_LEFT_6_PENALTY=8.086311 PRIMER_RIGHT_6_PENALTY=1.615453 PRIMER_LEFT_6_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_6_SEQUENCE=AGTACCCAGGGGATTTGCAA PRIMER_LEFT_6=30,27 PRIMER_RIGHT_6=185,20 PRIMER_LEFT_6_TM=58.914 PRIMER_RIGHT_6_TM=61.615 PRIMER_LEFT_6_GC_PERCENT=29.630 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_LEFT_6_SELF_ANY=6.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=6.00 PRIMER_RIGHT_6_SELF_END=6.00 PRIMER_LEFT_6_END_STABILITY=6.4000 PRIMER_RIGHT_6_END_STABILITY=8.8000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=4.00 PRIMER_PAIR_6_PRODUCT_SIZE=156 PRIMER_PAIR_7_PENALTY=9.908412 PRIMER_LEFT_7_PENALTY=8.200700 PRIMER_RIGHT_7_PENALTY=1.707711 PRIMER_LEFT_7_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTA PRIMER_RIGHT_7_SEQUENCE=GTACCCAGGGGATTTGCAA PRIMER_LEFT_7=0,23 PRIMER_RIGHT_7=184,19 PRIMER_LEFT_7_TM=54.799 PRIMER_RIGHT_7_TM=60.708 PRIMER_LEFT_7_GC_PERCENT=34.783 PRIMER_RIGHT_7_GC_PERCENT=52.632 PRIMER_LEFT_7_SELF_ANY=5.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=6.00 PRIMER_LEFT_7_END_STABILITY=5.7000 PRIMER_RIGHT_7_END_STABILITY=8.8000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PAIR_7_PRODUCT_SIZE=185 PRIMER_PAIR_8_PENALTY=9.971545 PRIMER_LEFT_8_PENALTY=8.211922 PRIMER_RIGHT_8_PENALTY=1.759622 PRIMER_LEFT_8_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAAT PRIMER_RIGHT_8_SEQUENCE=GGGGATTTGCAAGAGACTTT PRIMER_LEFT_8=0,26 PRIMER_RIGHT_8=177,20 PRIMER_LEFT_8_TM=57.788 PRIMER_RIGHT_8_TM=58.240 PRIMER_LEFT_8_GC_PERCENT=30.769 PRIMER_RIGHT_8_GC_PERCENT=45.000 PRIMER_LEFT_8_SELF_ANY=5.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=2.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_END_STABILITY=6.2000 PRIMER_RIGHT_8_END_STABILITY=6.7000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PAIR_8_PRODUCT_SIZE=178 PRIMER_PAIR_9_PENALTY=10.370560 PRIMER_LEFT_9_PENALTY=8.597369 PRIMER_RIGHT_9_PENALTY=1.773191 PRIMER_LEFT_9_SEQUENCE=CCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_9_SEQUENCE=AGGGGATTTGCAAGAGACTTT PRIMER_LEFT_9=31,26 PRIMER_RIGHT_9=178,21 PRIMER_LEFT_9_TM=57.403 PRIMER_RIGHT_9_TM=59.227 PRIMER_LEFT_9_GC_PERCENT=30.769 PRIMER_RIGHT_9_GC_PERCENT=42.857 PRIMER_LEFT_9_SELF_ANY=6.00 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_LEFT_9_SELF_END=6.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_END_STABILITY=6.4000 PRIMER_RIGHT_9_END_STABILITY=6.7000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=3.00 PRIMER_PAIR_9_PRODUCT_SIZE=148 PRIMER_PAIR_10_PENALTY=10.848910 PRIMER_LEFT_10_PENALTY=9.011349 PRIMER_RIGHT_10_PENALTY=1.837562 PRIMER_LEFT_10_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_10_SEQUENCE=TACCCAGGGGATTTGCAAG PRIMER_LEFT_10=1,26 PRIMER_RIGHT_10=183,19 PRIMER_LEFT_10_TM=56.989 PRIMER_RIGHT_10_TM=60.838 PRIMER_LEFT_10_GC_PERCENT=30.769 PRIMER_RIGHT_10_GC_PERCENT=52.632 PRIMER_LEFT_10_SELF_ANY=5.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=4.00 PRIMER_LEFT_10_END_STABILITY=7.2000 PRIMER_RIGHT_10_END_STABILITY=8.5000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=0.00 PRIMER_PAIR_10_PRODUCT_SIZE=183 PRIMER_PAIR_11_PENALTY=11.192893 PRIMER_LEFT_11_PENALTY=9.272253 PRIMER_RIGHT_11_PENALTY=1.920640 PRIMER_LEFT_11_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAAATGT PRIMER_RIGHT_11_SEQUENCE=CCAGGGGATTTGCAAGAGAC PRIMER_LEFT_11=1,27 PRIMER_RIGHT_11=180,20 PRIMER_LEFT_11_TM=57.728 PRIMER_RIGHT_11_TM=61.921 PRIMER_LEFT_11_GC_PERCENT=29.630 PRIMER_RIGHT_11_GC_PERCENT=55.000 PRIMER_LEFT_11_SELF_ANY=5.00 PRIMER_RIGHT_11_SELF_ANY=6.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_END_STABILITY=6.6000 PRIMER_RIGHT_11_END_STABILITY=6.1000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PAIR_11_PRODUCT_SIZE=180 PRIMER_PAIR_12_PENALTY=11.247669 PRIMER_LEFT_12_PENALTY=9.275372 PRIMER_RIGHT_12_PENALTY=1.972297 PRIMER_LEFT_12_SEQUENCE=CCTTTTAGATATGAATTAGCAAGTCAT PRIMER_RIGHT_12_SEQUENCE=GGGGATTTGCAAGAGACTTTG PRIMER_LEFT_12=31,27 PRIMER_RIGHT_12=177,21 PRIMER_LEFT_12_TM=57.725 PRIMER_RIGHT_12_TM=60.972 PRIMER_LEFT_12_GC_PERCENT=29.630 PRIMER_RIGHT_12_GC_PERCENT=47.619 PRIMER_LEFT_12_SELF_ANY=8.00 PRIMER_RIGHT_12_SELF_ANY=6.00 PRIMER_LEFT_12_SELF_END=8.00 PRIMER_RIGHT_12_SELF_END=5.00 PRIMER_LEFT_12_END_STABILITY=6.3000 PRIMER_RIGHT_12_END_STABILITY=7.3000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PAIR_12_PRODUCT_SIZE=147 PRIMER_PAIR_13_PENALTY=11.411862 PRIMER_LEFT_13_PENALTY=9.349327 PRIMER_RIGHT_13_PENALTY=2.062535 PRIMER_LEFT_13_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTC PRIMER_RIGHT_13_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_13=30,26 PRIMER_RIGHT_13=171,21 PRIMER_LEFT_13_TM=56.651 PRIMER_RIGHT_13_TM=58.937 PRIMER_LEFT_13_GC_PERCENT=30.769 PRIMER_RIGHT_13_GC_PERCENT=42.857 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_LEFT_13_SELF_END=4.00 PRIMER_RIGHT_13_SELF_END=3.00 PRIMER_LEFT_13_END_STABILITY=6.4000 PRIMER_RIGHT_13_END_STABILITY=6.7000 PRIMER_PAIR_13_COMPL_ANY=6.00 PRIMER_PAIR_13_COMPL_END=4.00 PRIMER_PAIR_13_PRODUCT_SIZE=142 PRIMER_PAIR_14_PENALTY=11.936750 PRIMER_LEFT_14_PENALTY=9.823274 PRIMER_RIGHT_14_PENALTY=2.113476 PRIMER_LEFT_14_SEQUENCE=TCCTTTTAGATATGAATTAGCAAG PRIMER_RIGHT_14_SEQUENCE=GGGATTTGCAAGAGACTTTGC PRIMER_LEFT_14=30,24 PRIMER_RIGHT_14=176,21 PRIMER_LEFT_14_TM=54.177 PRIMER_RIGHT_14_TM=61.113 PRIMER_LEFT_14_GC_PERCENT=29.167 PRIMER_RIGHT_14_GC_PERCENT=47.619 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=7.00 PRIMER_LEFT_14_SELF_END=1.00 PRIMER_RIGHT_14_SELF_END=7.00 PRIMER_LEFT_14_END_STABILITY=8.5000 PRIMER_RIGHT_14_END_STABILITY=8.8000 PRIMER_PAIR_14_COMPL_ANY=4.00 PRIMER_PAIR_14_COMPL_END=3.00 PRIMER_PAIR_14_PRODUCT_SIZE=147 PRIMER_PAIR_15_PENALTY=12.012534 PRIMER_LEFT_15_PENALTY=9.881359 PRIMER_RIGHT_15_PENALTY=2.131175 PRIMER_LEFT_15_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_15_SEQUENCE=CCAGGGGATTTGCAAGAGA PRIMER_LEFT_15=1,24 PRIMER_RIGHT_15=180,19 PRIMER_LEFT_15_TM=54.119 PRIMER_RIGHT_15_TM=61.131 PRIMER_LEFT_15_GC_PERCENT=29.167 PRIMER_RIGHT_15_GC_PERCENT=52.632 PRIMER_LEFT_15_SELF_ANY=5.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_END_STABILITY=6.0000 PRIMER_RIGHT_15_END_STABILITY=6.4000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=180 PRIMER_PAIR_16_PENALTY=12.065053 PRIMER_LEFT_16_PENALTY=9.918207 PRIMER_RIGHT_16_PENALTY=2.146847 PRIMER_LEFT_16_SEQUENCE=TCCTTTTAGATATGAATTAGCAA PRIMER_RIGHT_16_SEQUENCE=AGGGGATTTGCAAGAGACTT PRIMER_LEFT_16=30,23 PRIMER_RIGHT_16=178,20 PRIMER_LEFT_16_TM=53.082 PRIMER_RIGHT_16_TM=57.853 PRIMER_LEFT_16_GC_PERCENT=26.087 PRIMER_RIGHT_16_GC_PERCENT=45.000 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=3.00 PRIMER_LEFT_16_END_STABILITY=8.5000 PRIMER_RIGHT_16_END_STABILITY=6.4000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PAIR_16_PRODUCT_SIZE=149 PRIMER_PAIR_17_PENALTY=12.102186 PRIMER_LEFT_17_PENALTY=9.954194 PRIMER_RIGHT_17_PENALTY=2.147992 PRIMER_LEFT_17_SEQUENCE=CCTTTTAGATATGAATTAGCAAGTC PRIMER_RIGHT_17_SEQUENCE=TACCCAGGGGATTTGCAA PRIMER_LEFT_17=31,25 PRIMER_RIGHT_17=183,18 PRIMER_LEFT_17_TM=55.046 PRIMER_RIGHT_17_TM=59.852 PRIMER_LEFT_17_GC_PERCENT=32.000 PRIMER_RIGHT_17_GC_PERCENT=50.000 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=6.00 PRIMER_LEFT_17_SELF_END=4.00 PRIMER_RIGHT_17_SELF_END=6.00 PRIMER_LEFT_17_END_STABILITY=6.4000 PRIMER_RIGHT_17_END_STABILITY=8.8000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=5.00 PRIMER_PAIR_17_PRODUCT_SIZE=153 PRIMER_PAIR_18_PENALTY=12.151334 PRIMER_LEFT_18_PENALTY=9.954194 PRIMER_RIGHT_18_PENALTY=2.197140 PRIMER_LEFT_18_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGT PRIMER_RIGHT_18_SEQUENCE=TTGCAAGAGACTTTGCTCAGAC PRIMER_LEFT_18=30,25 PRIMER_RIGHT_18=171,22 PRIMER_LEFT_18_TM=55.046 PRIMER_RIGHT_18_TM=59.803 PRIMER_LEFT_18_GC_PERCENT=28.000 PRIMER_RIGHT_18_GC_PERCENT=45.455 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=7.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_END_STABILITY=6.7000 PRIMER_RIGHT_18_END_STABILITY=6.4000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=4.00 PRIMER_PAIR_18_PRODUCT_SIZE=142 PRIMER_PAIR_19_PENALTY=12.301028 PRIMER_LEFT_19_PENALTY=9.996186 PRIMER_RIGHT_19_PENALTY=2.304841 PRIMER_LEFT_19_SEQUENCE=ATCCTTTTAGATATGAATTAGCAAGTC PRIMER_RIGHT_19_SEQUENCE=TTTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_19=29,27 PRIMER_RIGHT_19=172,22 PRIMER_LEFT_19_TM=57.004 PRIMER_RIGHT_19_TM=60.305 PRIMER_LEFT_19_GC_PERCENT=29.630 PRIMER_RIGHT_19_GC_PERCENT=40.909 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=7.00 PRIMER_LEFT_19_SELF_END=4.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_END_STABILITY=6.4000 PRIMER_RIGHT_19_END_STABILITY=6.7000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=4.00 PRIMER_PAIR_19_PRODUCT_SIZE=144 PRIMER_PAIR_20_PENALTY=12.389179 PRIMER_LEFT_20_PENALTY=10.026416 PRIMER_RIGHT_20_PENALTY=2.362762 PRIMER_LEFT_20_SEQUENCE=TGTTATCCTTTTAGATATGAATTAGCA PRIMER_RIGHT_20_SEQUENCE=GATTTGCAAGAGACTTTGCTCA PRIMER_LEFT_20=25,27 PRIMER_RIGHT_20=174,22 PRIMER_LEFT_20_TM=56.974 PRIMER_RIGHT_20_TM=59.637 PRIMER_LEFT_20_GC_PERCENT=25.926 PRIMER_RIGHT_20_GC_PERCENT=40.909 PRIMER_LEFT_20_SELF_ANY=4.00 PRIMER_RIGHT_20_SELF_ANY=7.00 PRIMER_LEFT_20_SELF_END=2.00 PRIMER_RIGHT_20_SELF_END=5.00 PRIMER_LEFT_20_END_STABILITY=7.5000 PRIMER_RIGHT_20_END_STABILITY=8.2000 PRIMER_PAIR_20_COMPL_ANY=4.00 PRIMER_PAIR_20_COMPL_END=4.00 PRIMER_PAIR_20_PRODUCT_SIZE=150 PRIMER_PAIR_21_PENALTY=12.417677 PRIMER_LEFT_21_PENALTY=10.037140 PRIMER_RIGHT_21_PENALTY=2.380537 PRIMER_LEFT_21_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT PRIMER_RIGHT_21_SEQUENCE=GGATTTGCAAGAGACTTTGCTC PRIMER_LEFT_21=10,27 PRIMER_RIGHT_21=175,22 PRIMER_LEFT_21_TM=56.963 PRIMER_RIGHT_21_TM=60.381 PRIMER_LEFT_21_GC_PERCENT=25.926 PRIMER_RIGHT_21_GC_PERCENT=45.455 PRIMER_LEFT_21_SELF_ANY=6.00 PRIMER_RIGHT_21_SELF_ANY=7.00 PRIMER_LEFT_21_SELF_END=4.00 PRIMER_RIGHT_21_SELF_END=3.00 PRIMER_LEFT_21_END_STABILITY=7.3000 PRIMER_RIGHT_21_END_STABILITY=8.2000 PRIMER_PAIR_21_COMPL_ANY=5.00 PRIMER_PAIR_21_COMPL_END=1.00 PRIMER_PAIR_21_PRODUCT_SIZE=166 PRIMER_PAIR_22_PENALTY=12.639291 PRIMER_LEFT_22_PENALTY=10.231433 PRIMER_RIGHT_22_PENALTY=2.407858 PRIMER_LEFT_22_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTT PRIMER_RIGHT_22_SEQUENCE=GGATTTGCAAGAGACTTTGCT PRIMER_LEFT_22=10,26 PRIMER_RIGHT_22=175,21 PRIMER_LEFT_22_TM=55.769 PRIMER_RIGHT_22_TM=58.592 PRIMER_LEFT_22_GC_PERCENT=26.923 PRIMER_RIGHT_22_GC_PERCENT=42.857 PRIMER_LEFT_22_SELF_ANY=6.00 PRIMER_RIGHT_22_SELF_ANY=7.00 PRIMER_LEFT_22_SELF_END=6.00 PRIMER_RIGHT_22_SELF_END=5.00 PRIMER_LEFT_22_END_STABILITY=8.5000 PRIMER_RIGHT_22_END_STABILITY=8.5000 PRIMER_PAIR_22_COMPL_ANY=5.00 PRIMER_PAIR_22_COMPL_END=2.00 PRIMER_PAIR_22_PRODUCT_SIZE=166 PRIMER_PAIR_23_PENALTY=12.796734 PRIMER_LEFT_23_PENALTY=10.340510 PRIMER_RIGHT_23_PENALTY=2.456224 PRIMER_LEFT_23_SEQUENCE=ATCCTTTTAGATATGAATTAGCAAG PRIMER_RIGHT_23_SEQUENCE=GGATTTGCAAGAGACTTTGC PRIMER_LEFT_23=29,25 PRIMER_RIGHT_23=175,20 PRIMER_LEFT_23_TM=54.659 PRIMER_RIGHT_23_TM=57.544 PRIMER_LEFT_23_GC_PERCENT=28.000 PRIMER_RIGHT_23_GC_PERCENT=45.000 PRIMER_LEFT_23_SELF_ANY=4.00 PRIMER_RIGHT_23_SELF_ANY=7.00 PRIMER_LEFT_23_SELF_END=1.00 PRIMER_RIGHT_23_SELF_END=7.00 PRIMER_LEFT_23_END_STABILITY=8.5000 PRIMER_RIGHT_23_END_STABILITY=8.8000 PRIMER_PAIR_23_COMPL_ANY=4.00 PRIMER_PAIR_23_COMPL_END=3.00 PRIMER_PAIR_23_PRODUCT_SIZE=147 PRIMER_PAIR_24_PENALTY=12.897139 PRIMER_LEFT_24_PENALTY=10.341962 PRIMER_RIGHT_24_PENALTY=2.555177 PRIMER_LEFT_24_SEQUENCE=AGAAAGTGAAAGTAAATGTTATCCTTT PRIMER_RIGHT_24_SEQUENCE=TTTGCAAGAGACTTTGCTCAG PRIMER_LEFT_24=9,27 PRIMER_RIGHT_24=172,21 PRIMER_LEFT_24_TM=56.658 PRIMER_RIGHT_24_TM=58.445 PRIMER_LEFT_24_GC_PERCENT=25.926 PRIMER_RIGHT_24_GC_PERCENT=42.857 PRIMER_LEFT_24_SELF_ANY=6.00 PRIMER_RIGHT_24_SELF_ANY=7.00 PRIMER_LEFT_24_SELF_END=6.00 PRIMER_RIGHT_24_SELF_END=3.00 PRIMER_LEFT_24_END_STABILITY=8.5000 PRIMER_RIGHT_24_END_STABILITY=6.7000 PRIMER_PAIR_24_COMPL_ANY=5.00 PRIMER_PAIR_24_COMPL_END=0.00 PRIMER_PAIR_24_PRODUCT_SIZE=164 PRIMER_PAIR_25_PENALTY=12.932691 PRIMER_LEFT_25_PENALTY=10.365708 PRIMER_RIGHT_25_PENALTY=2.566983 PRIMER_LEFT_25_SEQUENCE=ATCCTTTTAGATATGAATTAGCAA PRIMER_RIGHT_25_SEQUENCE=TAGTACCCAGGGGATTTGCAA PRIMER_LEFT_25=29,24 PRIMER_RIGHT_25=186,21 PRIMER_LEFT_25_TM=53.634 PRIMER_RIGHT_25_TM=61.567 PRIMER_LEFT_25_GC_PERCENT=25.000 PRIMER_RIGHT_25_GC_PERCENT=47.619 PRIMER_LEFT_25_SELF_ANY=4.00 PRIMER_RIGHT_25_SELF_ANY=6.00 PRIMER_LEFT_25_SELF_END=2.00 PRIMER_RIGHT_25_SELF_END=6.00 PRIMER_LEFT_25_END_STABILITY=8.5000 PRIMER_RIGHT_25_END_STABILITY=8.8000 PRIMER_PAIR_25_COMPL_ANY=4.00 PRIMER_PAIR_25_COMPL_END=4.00 PRIMER_PAIR_25_PRODUCT_SIZE=158 PRIMER_PAIR_26_PENALTY=12.952862 PRIMER_LEFT_26_PENALTY=10.385879 PRIMER_RIGHT_26_PENALTY=2.566983 PRIMER_LEFT_26_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAA PRIMER_RIGHT_26_SEQUENCE=TTAGTACCCAGGGGATTTGCA PRIMER_LEFT_26=1,23 PRIMER_RIGHT_26=187,21 PRIMER_LEFT_26_TM=52.614 PRIMER_RIGHT_26_TM=61.567 PRIMER_LEFT_26_GC_PERCENT=30.435 PRIMER_RIGHT_26_GC_PERCENT=47.619 PRIMER_LEFT_26_SELF_ANY=5.00 PRIMER_RIGHT_26_SELF_ANY=5.00 PRIMER_LEFT_26_SELF_END=1.00 PRIMER_RIGHT_26_SELF_END=4.00 PRIMER_LEFT_26_END_STABILITY=5.7000 PRIMER_RIGHT_26_END_STABILITY=8.8000 PRIMER_PAIR_26_COMPL_ANY=3.00 PRIMER_PAIR_26_COMPL_END=1.00 PRIMER_PAIR_26_PRODUCT_SIZE=187 PRIMER_PAIR_27_PENALTY=12.982286 PRIMER_LEFT_27_PENALTY=10.387986 PRIMER_RIGHT_27_PENALTY=2.594300 PRIMER_LEFT_27_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAAAT PRIMER_RIGHT_27_SEQUENCE=TGCAAGAGACTTTGCTCAGA PRIMER_LEFT_27=1,25 PRIMER_RIGHT_27=170,20 PRIMER_LEFT_27_TM=54.612 PRIMER_RIGHT_27_TM=57.406 PRIMER_LEFT_27_GC_PERCENT=28.000 PRIMER_RIGHT_27_GC_PERCENT=45.000 PRIMER_LEFT_27_SELF_ANY=5.00 PRIMER_RIGHT_27_SELF_ANY=7.00 PRIMER_LEFT_27_SELF_END=2.00 PRIMER_RIGHT_27_SELF_END=3.00 PRIMER_LEFT_27_END_STABILITY=6.2000 PRIMER_RIGHT_27_END_STABILITY=6.7000 PRIMER_PAIR_27_COMPL_ANY=5.00 PRIMER_PAIR_27_COMPL_END=1.00 PRIMER_PAIR_27_PRODUCT_SIZE=170 PRIMER_PAIR_28_PENALTY=13.010954 PRIMER_LEFT_28_PENALTY=10.403980 PRIMER_RIGHT_28_PENALTY=2.606973 PRIMER_LEFT_28_SEQUENCE=CCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_28_SEQUENCE=GGGATTTGCAAGAGACTTTG PRIMER_LEFT_28=1,21 PRIMER_RIGHT_28=176,20 PRIMER_LEFT_28_TM=50.596 PRIMER_RIGHT_28_TM=57.393 PRIMER_LEFT_28_GC_PERCENT=33.333 PRIMER_RIGHT_28_GC_PERCENT=45.000 PRIMER_LEFT_28_SELF_ANY=5.00 PRIMER_RIGHT_28_SELF_ANY=6.00 PRIMER_LEFT_28_SELF_END=3.00 PRIMER_RIGHT_28_SELF_END=5.00 PRIMER_LEFT_28_END_STABILITY=6.7000 PRIMER_RIGHT_28_END_STABILITY=7.3000 PRIMER_PAIR_28_COMPL_ANY=5.00 PRIMER_PAIR_28_COMPL_END=4.00 PRIMER_PAIR_28_PRODUCT_SIZE=176 PRIMER_PAIR_29_PENALTY=13.129252 PRIMER_LEFT_29_PENALTY=10.513822 PRIMER_RIGHT_29_PENALTY=2.615430 PRIMER_LEFT_29_SEQUENCE=TCCTTTTAGATATGAATTAGCA PRIMER_RIGHT_29_SEQUENCE=GCAAGAGACTTTGCTCAGACA PRIMER_LEFT_29=30,22 PRIMER_RIGHT_29=169,21 PRIMER_LEFT_29_TM=51.486 PRIMER_RIGHT_29_TM=58.385 PRIMER_LEFT_29_GC_PERCENT=27.273 PRIMER_RIGHT_29_GC_PERCENT=47.619 PRIMER_LEFT_29_SELF_ANY=4.00 PRIMER_RIGHT_29_SELF_ANY=7.00 PRIMER_LEFT_29_SELF_END=2.00 PRIMER_RIGHT_29_SELF_END=3.00 PRIMER_LEFT_29_END_STABILITY=7.5000 PRIMER_RIGHT_29_END_STABILITY=6.4000 PRIMER_PAIR_29_COMPL_ANY=4.00 PRIMER_PAIR_29_COMPL_END=4.00 PRIMER_PAIR_29_PRODUCT_SIZE=140 PRIMER_PAIR_30_PENALTY=13.139112 PRIMER_LEFT_30_PENALTY=10.523682 PRIMER_RIGHT_30_PENALTY=2.615430 PRIMER_LEFT_30_SEQUENCE=ATCCTTTTAGATATGAATTAGCAAGT PRIMER_RIGHT_30_SEQUENCE=TGCAAGAGACTTTGCTCAGAC PRIMER_LEFT_30=29,26 PRIMER_RIGHT_30=170,21 PRIMER_LEFT_30_TM=55.476 PRIMER_RIGHT_30_TM=58.385 PRIMER_LEFT_30_GC_PERCENT=26.923 PRIMER_RIGHT_30_GC_PERCENT=47.619 PRIMER_LEFT_30_SELF_ANY=4.00 PRIMER_RIGHT_30_SELF_ANY=7.00 PRIMER_LEFT_30_SELF_END=2.00 PRIMER_RIGHT_30_SELF_END=2.00 PRIMER_LEFT_30_END_STABILITY=6.7000 PRIMER_RIGHT_30_END_STABILITY=6.4000 PRIMER_PAIR_30_COMPL_ANY=5.00 PRIMER_PAIR_30_COMPL_END=4.00 PRIMER_PAIR_30_PRODUCT_SIZE=142 PRIMER_PAIR_31_PENALTY=13.172588 PRIMER_LEFT_31_PENALTY=10.534347 PRIMER_RIGHT_31_PENALTY=2.638241 PRIMER_LEFT_31_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTT PRIMER_RIGHT_31_SEQUENCE=TACCCAGGGGATTTGCAAGA PRIMER_LEFT_31=10,25 PRIMER_RIGHT_31=183,20 PRIMER_LEFT_31_TM=54.466 PRIMER_RIGHT_31_TM=62.638 PRIMER_LEFT_31_GC_PERCENT=28.000 PRIMER_RIGHT_31_GC_PERCENT=50.000 PRIMER_LEFT_31_SELF_ANY=4.00 PRIMER_RIGHT_31_SELF_ANY=6.00 PRIMER_LEFT_31_SELF_END=4.00 PRIMER_RIGHT_31_SELF_END=4.00 PRIMER_LEFT_31_END_STABILITY=8.2000 PRIMER_RIGHT_31_END_STABILITY=7.0000 PRIMER_PAIR_31_COMPL_ANY=4.00 PRIMER_PAIR_31_COMPL_END=2.00 PRIMER_PAIR_31_PRODUCT_SIZE=174 PRIMER_PAIR_32_PENALTY=13.205919 PRIMER_LEFT_32_PENALTY=10.552307 PRIMER_RIGHT_32_PENALTY=2.653612 PRIMER_LEFT_32_SEQUENCE=AAAGTGAAAGTAAATGTTATCCTTTT PRIMER_RIGHT_32_SEQUENCE=TTTGCAAGAGACTTTGCTCA PRIMER_LEFT_32=11,26 PRIMER_RIGHT_32=172,20 PRIMER_LEFT_32_TM=55.448 PRIMER_RIGHT_32_TM=57.346 PRIMER_LEFT_32_GC_PERCENT=23.077 PRIMER_RIGHT_32_GC_PERCENT=40.000 PRIMER_LEFT_32_SELF_ANY=6.00 PRIMER_RIGHT_32_SELF_ANY=7.00 PRIMER_LEFT_32_SELF_END=4.00 PRIMER_RIGHT_32_SELF_END=5.00 PRIMER_LEFT_32_END_STABILITY=7.3000 PRIMER_RIGHT_32_END_STABILITY=8.2000 PRIMER_PAIR_32_COMPL_ANY=5.00 PRIMER_PAIR_32_COMPL_END=1.00 PRIMER_PAIR_32_PRODUCT_SIZE=162 PRIMER_PAIR_33_PENALTY=13.260761 PRIMER_LEFT_33_PENALTY=10.556557 PRIMER_RIGHT_33_PENALTY=2.704204 PRIMER_LEFT_33_SEQUENCE=AGAAAGTGAAAGTAAATGTTATCCTT PRIMER_RIGHT_33_SEQUENCE=GTACCCAGGGGATTTGCA PRIMER_LEFT_33=9,26 PRIMER_RIGHT_33=184,18 PRIMER_LEFT_33_TM=55.443 PRIMER_RIGHT_33_TM=59.296 PRIMER_LEFT_33_GC_PERCENT=26.923 PRIMER_RIGHT_33_GC_PERCENT=55.556 PRIMER_LEFT_33_SELF_ANY=4.00 PRIMER_RIGHT_33_SELF_ANY=5.00 PRIMER_LEFT_33_SELF_END=4.00 PRIMER_RIGHT_33_SELF_END=4.00 PRIMER_LEFT_33_END_STABILITY=8.2000 PRIMER_RIGHT_33_END_STABILITY=8.8000 PRIMER_PAIR_33_COMPL_ANY=4.00 PRIMER_PAIR_33_COMPL_END=1.00 PRIMER_PAIR_33_PRODUCT_SIZE=176 PRIMER_PAIR_34_PENALTY=13.302494 PRIMER_LEFT_34_PENALTY=10.587869 PRIMER_RIGHT_34_PENALTY=2.714625 PRIMER_LEFT_34_SEQUENCE=GCCTTCATTAGAAAGTGAA PRIMER_RIGHT_34_SEQUENCE=GCAAGAGACTTTGCTCAGACAC PRIMER_LEFT_34=0,19 PRIMER_RIGHT_34=169,22 PRIMER_LEFT_34_TM=50.412 PRIMER_RIGHT_34_TM=59.285 PRIMER_LEFT_34_GC_PERCENT=36.842 PRIMER_RIGHT_34_GC_PERCENT=50.000 PRIMER_LEFT_34_SELF_ANY=5.00 PRIMER_RIGHT_34_SELF_ANY=7.00 PRIMER_LEFT_34_SELF_END=5.00 PRIMER_RIGHT_34_SELF_END=2.00 PRIMER_LEFT_34_END_STABILITY=6.7000 PRIMER_RIGHT_34_END_STABILITY=6.1000 PRIMER_PAIR_34_COMPL_ANY=5.00 PRIMER_PAIR_34_COMPL_END=1.00 PRIMER_PAIR_34_PRODUCT_SIZE=170 PRIMER_PAIR_35_PENALTY=13.377380 PRIMER_LEFT_35_PENALTY=10.592921 PRIMER_RIGHT_35_PENALTY=2.784458 PRIMER_LEFT_35_SEQUENCE=CCTTTTAGATATGAATTAGCAAG PRIMER_RIGHT_35_SEQUENCE=CAGGGGATTTGCAAGAGAC PRIMER_LEFT_35=31,23 PRIMER_RIGHT_35=179,19 PRIMER_LEFT_35_TM=52.407 PRIMER_RIGHT_35_TM=58.216 PRIMER_LEFT_35_GC_PERCENT=30.435 PRIMER_RIGHT_35_GC_PERCENT=52.632 PRIMER_LEFT_35_SELF_ANY=4.00 PRIMER_RIGHT_35_SELF_ANY=6.00 PRIMER_LEFT_35_SELF_END=1.00 PRIMER_RIGHT_35_SELF_END=2.00 PRIMER_LEFT_35_END_STABILITY=8.5000 PRIMER_RIGHT_35_END_STABILITY=6.1000 PRIMER_PAIR_35_COMPL_ANY=4.00 PRIMER_PAIR_35_COMPL_END=1.00 PRIMER_PAIR_35_PRODUCT_SIZE=149 PRIMER_PAIR_36_PENALTY=13.478095 PRIMER_LEFT_36_PENALTY=10.640141 PRIMER_RIGHT_36_PENALTY=2.837954 PRIMER_LEFT_36_SEQUENCE=CCTTCATTAGAAAGTGAAAG PRIMER_RIGHT_36_SEQUENCE=CCAGGGGATTTGCAAGAG PRIMER_LEFT_36=1,20 PRIMER_RIGHT_36=180,18 PRIMER_LEFT_36_TM=49.360 PRIMER_RIGHT_36_TM=59.162 PRIMER_LEFT_36_GC_PERCENT=35.000 PRIMER_RIGHT_36_GC_PERCENT=55.556 PRIMER_LEFT_36_SELF_ANY=5.00 PRIMER_RIGHT_36_SELF_ANY=6.00 PRIMER_LEFT_36_SELF_END=5.00 PRIMER_RIGHT_36_SELF_END=2.00 PRIMER_LEFT_36_END_STABILITY=7.0000 PRIMER_RIGHT_36_END_STABILITY=6.7000 PRIMER_PAIR_36_COMPL_ANY=3.00 PRIMER_PAIR_36_COMPL_END=0.00 PRIMER_PAIR_36_PRODUCT_SIZE=180 PRIMER_PAIR_37_PENALTY=13.502637 PRIMER_LEFT_37_PENALTY=10.642398 PRIMER_RIGHT_37_PENALTY=2.860239 PRIMER_LEFT_37_SEQUENCE=CCTTTTAGATATGAATTAGCAAGT PRIMER_RIGHT_37_SEQUENCE=ACCCAGGGGATTTGCAAG PRIMER_LEFT_37=31,24 PRIMER_RIGHT_37=182,18 PRIMER_LEFT_37_TM=53.358 PRIMER_RIGHT_37_TM=60.860 PRIMER_LEFT_37_GC_PERCENT=29.167 PRIMER_RIGHT_37_GC_PERCENT=55.556 PRIMER_LEFT_37_SELF_ANY=4.00 PRIMER_RIGHT_37_SELF_ANY=6.00 PRIMER_LEFT_37_SELF_END=2.00 PRIMER_RIGHT_37_SELF_END=4.00 PRIMER_LEFT_37_END_STABILITY=6.7000 PRIMER_RIGHT_37_END_STABILITY=8.5000 PRIMER_PAIR_37_COMPL_ANY=4.00 PRIMER_PAIR_37_COMPL_END=3.00 PRIMER_PAIR_37_PRODUCT_SIZE=152 PRIMER_PAIR_38_PENALTY=13.815407 PRIMER_LEFT_38_PENALTY=10.738861 PRIMER_RIGHT_38_PENALTY=3.076546 PRIMER_LEFT_38_SEQUENCE=CTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_38_SEQUENCE=TTGCAAGAGACTTTGCTCAG PRIMER_LEFT_38=32,25 PRIMER_RIGHT_38=171,20 PRIMER_LEFT_38_TM=54.261 PRIMER_RIGHT_38_TM=56.923 PRIMER_LEFT_38_GC_PERCENT=28.000 PRIMER_RIGHT_38_GC_PERCENT=45.000 PRIMER_LEFT_38_SELF_ANY=6.00 PRIMER_RIGHT_38_SELF_ANY=7.00 PRIMER_LEFT_38_SELF_END=6.00 PRIMER_RIGHT_38_SELF_END=3.00 PRIMER_LEFT_38_END_STABILITY=6.4000 PRIMER_RIGHT_38_END_STABILITY=6.7000 PRIMER_PAIR_38_COMPL_ANY=6.00 PRIMER_PAIR_38_COMPL_END=4.00 PRIMER_PAIR_38_PRODUCT_SIZE=140 PRIMER_PAIR_39_PENALTY=14.003303 PRIMER_LEFT_39_PENALTY=10.794919 PRIMER_RIGHT_39_PENALTY=3.208384 PRIMER_LEFT_39_SEQUENCE=CCTTTTAGATATGAATTAGCAA PRIMER_RIGHT_39_SEQUENCE=ATTTGCAAGAGACTTTGCTCAG PRIMER_LEFT_39=31,22 PRIMER_RIGHT_39=173,22 PRIMER_LEFT_39_TM=51.205 PRIMER_RIGHT_39_TM=58.792 PRIMER_LEFT_39_GC_PERCENT=27.273 PRIMER_RIGHT_39_GC_PERCENT=40.909 PRIMER_LEFT_39_SELF_ANY=4.00 PRIMER_RIGHT_39_SELF_ANY=7.00 PRIMER_LEFT_39_SELF_END=2.00 PRIMER_RIGHT_39_SELF_END=3.00 PRIMER_LEFT_39_END_STABILITY=8.5000 PRIMER_RIGHT_39_END_STABILITY=6.7000 PRIMER_PAIR_39_COMPL_ANY=5.00 PRIMER_PAIR_39_COMPL_END=4.00 PRIMER_PAIR_39_PRODUCT_SIZE=143 = PRIMER_MIN_THREE_PRIME_DISTANCE=1 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=23 PRIMER_RIGHT_NUM_RETURNED=23 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=23 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=8.114341 PRIMER_LEFT_1_PENALTY=7.398215 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_1=0,22 PRIMER_RIGHT_1=179,20 PRIMER_LEFT_1_TM=54.602 PRIMER_RIGHT_1_TM=59.284 PRIMER_LEFT_1_GC_PERCENT=36.364 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=180 PRIMER_PAIR_2_PENALTY=8.504534 PRIMER_LEFT_2_PENALTY=7.407966 PRIMER_RIGHT_2_PENALTY=1.096569 PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAG PRIMER_RIGHT_2_SEQUENCE=TTAGTACCCAGGGGATTTGC PRIMER_LEFT_2=0,21 PRIMER_RIGHT_2=187,20 PRIMER_LEFT_2_TM=53.592 PRIMER_RIGHT_2_TM=58.903 PRIMER_LEFT_2_GC_PERCENT=38.095 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.8000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=188 PRIMER_PAIR_3_PENALTY=9.153890 PRIMER_LEFT_3_PENALTY=7.538496 PRIMER_RIGHT_3_PENALTY=1.615394 PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_3_SEQUENCE=CAGGGGATTTGCAAGAGACTT PRIMER_LEFT_3=0,25 PRIMER_RIGHT_3=179,21 PRIMER_LEFT_3_TM=57.462 PRIMER_RIGHT_3_TM=60.615 PRIMER_LEFT_3_GC_PERCENT=32.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=6.0000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=180 PRIMER_PAIR_4_PENALTY=9.309894 PRIMER_LEFT_4_PENALTY=7.694441 PRIMER_RIGHT_4_PENALTY=1.615453 PRIMER_LEFT_4_SEQUENCE=GCCTTCATTAGAAAGTGAAA PRIMER_RIGHT_4_SEQUENCE=GTACCCAGGGGATTTGCAAG PRIMER_LEFT_4=0,20 PRIMER_RIGHT_4=184,20 PRIMER_LEFT_4_TM=52.306 PRIMER_RIGHT_4_TM=61.615 PRIMER_LEFT_4_GC_PERCENT=35.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=185 PRIMER_PAIR_5_PENALTY=9.422419 PRIMER_LEFT_5_PENALTY=7.806965 PRIMER_RIGHT_5_PENALTY=1.615453 PRIMER_LEFT_5_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAA PRIMER_RIGHT_5_SEQUENCE=AGTACCCAGGGGATTTGCAA PRIMER_LEFT_5=0,24 PRIMER_RIGHT_5=185,20 PRIMER_LEFT_5_TM=56.193 PRIMER_RIGHT_5_TM=61.615 PRIMER_LEFT_5_GC_PERCENT=33.333 PRIMER_RIGHT_5_GC_PERCENT=50.000 PRIMER_LEFT_5_SELF_ANY=5.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=6.00 PRIMER_LEFT_5_END_STABILITY=5.7000 PRIMER_RIGHT_5_END_STABILITY=8.8000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PAIR_5_PRODUCT_SIZE=186 PRIMER_PAIR_6_PENALTY=9.845933 PRIMER_LEFT_6_PENALTY=8.086311 PRIMER_RIGHT_6_PENALTY=1.759622 PRIMER_LEFT_6_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_6_SEQUENCE=GGGGATTTGCAAGAGACTTT PRIMER_LEFT_6=30,27 PRIMER_RIGHT_6=177,20 PRIMER_LEFT_6_TM=58.914 PRIMER_RIGHT_6_TM=58.240 PRIMER_LEFT_6_GC_PERCENT=29.630 PRIMER_RIGHT_6_GC_PERCENT=45.000 PRIMER_LEFT_6_SELF_ANY=6.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=6.00 PRIMER_RIGHT_6_SELF_END=3.00 PRIMER_LEFT_6_END_STABILITY=6.4000 PRIMER_RIGHT_6_END_STABILITY=6.7000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=148 PRIMER_PAIR_7_PENALTY=10.121341 PRIMER_LEFT_7_PENALTY=8.200700 PRIMER_RIGHT_7_PENALTY=1.920640 PRIMER_LEFT_7_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTA PRIMER_RIGHT_7_SEQUENCE=CCAGGGGATTTGCAAGAGAC PRIMER_LEFT_7=0,23 PRIMER_RIGHT_7=180,20 PRIMER_LEFT_7_TM=54.799 PRIMER_RIGHT_7_TM=61.921 PRIMER_LEFT_7_GC_PERCENT=34.783 PRIMER_RIGHT_7_GC_PERCENT=55.000 PRIMER_LEFT_7_SELF_ANY=5.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_END_STABILITY=5.7000 PRIMER_RIGHT_7_END_STABILITY=6.1000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PAIR_7_PRODUCT_SIZE=181 PRIMER_PAIR_8_PENALTY=10.184220 PRIMER_LEFT_8_PENALTY=8.211922 PRIMER_RIGHT_8_PENALTY=1.972297 PRIMER_LEFT_8_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAAT PRIMER_RIGHT_8_SEQUENCE=GGGGATTTGCAAGAGACTTTG PRIMER_LEFT_8=0,26 PRIMER_RIGHT_8=177,21 PRIMER_LEFT_8_TM=57.788 PRIMER_RIGHT_8_TM=60.972 PRIMER_LEFT_8_GC_PERCENT=30.769 PRIMER_RIGHT_8_GC_PERCENT=47.619 PRIMER_LEFT_8_SELF_ANY=5.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=2.00 PRIMER_RIGHT_8_SELF_END=5.00 PRIMER_LEFT_8_END_STABILITY=6.2000 PRIMER_RIGHT_8_END_STABILITY=7.3000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PAIR_8_PRODUCT_SIZE=178 PRIMER_PAIR_9_PENALTY=11.334788 PRIMER_LEFT_9_PENALTY=9.272253 PRIMER_RIGHT_9_PENALTY=2.062535 PRIMER_LEFT_9_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAAATGT PRIMER_RIGHT_9_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_9=1,27 PRIMER_RIGHT_9=171,21 PRIMER_LEFT_9_TM=57.728 PRIMER_RIGHT_9_TM=58.937 PRIMER_LEFT_9_GC_PERCENT=29.630 PRIMER_RIGHT_9_GC_PERCENT=42.857 PRIMER_LEFT_9_SELF_ANY=5.00 PRIMER_RIGHT_9_SELF_ANY=7.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_END_STABILITY=6.6000 PRIMER_RIGHT_9_END_STABILITY=6.7000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=2.00 PRIMER_PAIR_9_PRODUCT_SIZE=171 PRIMER_PAIR_10_PENALTY=11.388848 PRIMER_LEFT_10_PENALTY=9.275372 PRIMER_RIGHT_10_PENALTY=2.113476 PRIMER_LEFT_10_SEQUENCE=CCTTTTAGATATGAATTAGCAAGTCAT PRIMER_RIGHT_10_SEQUENCE=GGGATTTGCAAGAGACTTTGC PRIMER_LEFT_10=31,27 PRIMER_RIGHT_10=176,21 PRIMER_LEFT_10_TM=57.725 PRIMER_RIGHT_10_TM=61.113 PRIMER_LEFT_10_GC_PERCENT=29.630 PRIMER_RIGHT_10_GC_PERCENT=47.619 PRIMER_LEFT_10_SELF_ANY=8.00 PRIMER_RIGHT_10_SELF_ANY=7.00 PRIMER_LEFT_10_SELF_END=8.00 PRIMER_RIGHT_10_SELF_END=7.00 PRIMER_LEFT_10_END_STABILITY=6.3000 PRIMER_RIGHT_10_END_STABILITY=8.8000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=4.00 PRIMER_PAIR_10_PRODUCT_SIZE=146 PRIMER_PAIR_11_PENALTY=11.480502 PRIMER_LEFT_11_PENALTY=9.349327 PRIMER_RIGHT_11_PENALTY=2.131175 PRIMER_LEFT_11_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTC PRIMER_RIGHT_11_SEQUENCE=CCAGGGGATTTGCAAGAGA PRIMER_LEFT_11=30,26 PRIMER_RIGHT_11=180,19 PRIMER_LEFT_11_TM=56.651 PRIMER_RIGHT_11_TM=61.131 PRIMER_LEFT_11_GC_PERCENT=30.769 PRIMER_RIGHT_11_GC_PERCENT=52.632 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=6.00 PRIMER_LEFT_11_SELF_END=4.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_END_STABILITY=6.4000 PRIMER_RIGHT_11_END_STABILITY=6.4000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=3.00 PRIMER_PAIR_11_PRODUCT_SIZE=151 PRIMER_PAIR_12_PENALTY=12.020413 PRIMER_LEFT_12_PENALTY=9.823274 PRIMER_RIGHT_12_PENALTY=2.197140 PRIMER_LEFT_12_SEQUENCE=TCCTTTTAGATATGAATTAGCAAG PRIMER_RIGHT_12_SEQUENCE=TTGCAAGAGACTTTGCTCAGAC PRIMER_LEFT_12=30,24 PRIMER_RIGHT_12=171,22 PRIMER_LEFT_12_TM=54.177 PRIMER_RIGHT_12_TM=59.803 PRIMER_LEFT_12_GC_PERCENT=29.167 PRIMER_RIGHT_12_GC_PERCENT=45.455 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=7.00 PRIMER_LEFT_12_SELF_END=1.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_END_STABILITY=8.5000 PRIMER_RIGHT_12_END_STABILITY=6.4000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PAIR_12_PRODUCT_SIZE=142 PRIMER_PAIR_13_PENALTY=12.280969 PRIMER_LEFT_13_PENALTY=9.918207 PRIMER_RIGHT_13_PENALTY=2.362762 PRIMER_LEFT_13_SEQUENCE=TCCTTTTAGATATGAATTAGCAA PRIMER_RIGHT_13_SEQUENCE=GATTTGCAAGAGACTTTGCTCA PRIMER_LEFT_13=30,23 PRIMER_RIGHT_13=174,22 PRIMER_LEFT_13_TM=53.082 PRIMER_RIGHT_13_TM=59.637 PRIMER_LEFT_13_GC_PERCENT=26.087 PRIMER_RIGHT_13_GC_PERCENT=40.909 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=5.00 PRIMER_LEFT_13_END_STABILITY=8.5000 PRIMER_RIGHT_13_END_STABILITY=8.2000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=5.00 PRIMER_PAIR_13_PRODUCT_SIZE=145 PRIMER_PAIR_14_PENALTY=12.334731 PRIMER_LEFT_14_PENALTY=9.954194 PRIMER_RIGHT_14_PENALTY=2.380537 PRIMER_LEFT_14_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGT PRIMER_RIGHT_14_SEQUENCE=GGATTTGCAAGAGACTTTGCTC PRIMER_LEFT_14=30,25 PRIMER_RIGHT_14=175,22 PRIMER_LEFT_14_TM=55.046 PRIMER_RIGHT_14_TM=60.381 PRIMER_LEFT_14_GC_PERCENT=28.000 PRIMER_RIGHT_14_GC_PERCENT=45.455 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=7.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=3.00 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=8.2000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=4.00 PRIMER_PAIR_14_PRODUCT_SIZE=146 PRIMER_PAIR_15_PENALTY=12.434275 PRIMER_LEFT_15_PENALTY=10.026416 PRIMER_RIGHT_15_PENALTY=2.407858 PRIMER_LEFT_15_SEQUENCE=TGTTATCCTTTTAGATATGAATTAGCA PRIMER_RIGHT_15_SEQUENCE=GGATTTGCAAGAGACTTTGCT PRIMER_LEFT_15=25,27 PRIMER_RIGHT_15=175,21 PRIMER_LEFT_15_TM=56.974 PRIMER_RIGHT_15_TM=58.592 PRIMER_LEFT_15_GC_PERCENT=25.926 PRIMER_RIGHT_15_GC_PERCENT=42.857 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=7.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=5.00 PRIMER_LEFT_15_END_STABILITY=7.5000 PRIMER_RIGHT_15_END_STABILITY=8.5000 PRIMER_PAIR_15_COMPL_ANY=4.00 PRIMER_PAIR_15_COMPL_END=4.00 PRIMER_PAIR_15_PRODUCT_SIZE=151 PRIMER_PAIR_16_PENALTY=12.592317 PRIMER_LEFT_16_PENALTY=10.037140 PRIMER_RIGHT_16_PENALTY=2.555177 PRIMER_LEFT_16_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT PRIMER_RIGHT_16_SEQUENCE=TTTGCAAGAGACTTTGCTCAG PRIMER_LEFT_16=10,27 PRIMER_RIGHT_16=172,21 PRIMER_LEFT_16_TM=56.963 PRIMER_RIGHT_16_TM=58.445 PRIMER_LEFT_16_GC_PERCENT=25.926 PRIMER_RIGHT_16_GC_PERCENT=42.857 PRIMER_LEFT_16_SELF_ANY=6.00 PRIMER_RIGHT_16_SELF_ANY=7.00 PRIMER_LEFT_16_SELF_END=4.00 PRIMER_RIGHT_16_SELF_END=3.00 PRIMER_LEFT_16_END_STABILITY=7.3000 PRIMER_RIGHT_16_END_STABILITY=6.7000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=0.00 PRIMER_PAIR_16_PRODUCT_SIZE=163 PRIMER_PAIR_17_PENALTY=12.846863 PRIMER_LEFT_17_PENALTY=10.231433 PRIMER_RIGHT_17_PENALTY=2.615430 PRIMER_LEFT_17_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTT PRIMER_RIGHT_17_SEQUENCE=GCAAGAGACTTTGCTCAGACA PRIMER_LEFT_17=10,26 PRIMER_RIGHT_17=169,21 PRIMER_LEFT_17_TM=55.769 PRIMER_RIGHT_17_TM=58.385 PRIMER_LEFT_17_GC_PERCENT=26.923 PRIMER_RIGHT_17_GC_PERCENT=47.619 PRIMER_LEFT_17_SELF_ANY=6.00 PRIMER_RIGHT_17_SELF_ANY=7.00 PRIMER_LEFT_17_SELF_END=6.00 PRIMER_RIGHT_17_SELF_END=3.00 PRIMER_LEFT_17_END_STABILITY=8.5000 PRIMER_RIGHT_17_END_STABILITY=6.4000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PAIR_17_PRODUCT_SIZE=160 PRIMER_PAIR_18_PENALTY=13.172588 PRIMER_LEFT_18_PENALTY=10.534347 PRIMER_RIGHT_18_PENALTY=2.638241 PRIMER_LEFT_18_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTT PRIMER_RIGHT_18_SEQUENCE=TACCCAGGGGATTTGCAAGA PRIMER_LEFT_18=10,25 PRIMER_RIGHT_18=183,20 PRIMER_LEFT_18_TM=54.466 PRIMER_RIGHT_18_TM=62.638 PRIMER_LEFT_18_GC_PERCENT=28.000 PRIMER_RIGHT_18_GC_PERCENT=50.000 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=6.00 PRIMER_LEFT_18_SELF_END=4.00 PRIMER_RIGHT_18_SELF_END=4.00 PRIMER_LEFT_18_END_STABILITY=8.2000 PRIMER_RIGHT_18_END_STABILITY=7.0000 PRIMER_PAIR_18_COMPL_ANY=4.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PAIR_18_PRODUCT_SIZE=174 PRIMER_PAIR_19_PENALTY=13.302494 PRIMER_LEFT_19_PENALTY=10.587869 PRIMER_RIGHT_19_PENALTY=2.714625 PRIMER_LEFT_19_SEQUENCE=GCCTTCATTAGAAAGTGAA PRIMER_RIGHT_19_SEQUENCE=GCAAGAGACTTTGCTCAGACAC PRIMER_LEFT_19=0,19 PRIMER_RIGHT_19=169,22 PRIMER_LEFT_19_TM=50.412 PRIMER_RIGHT_19_TM=59.285 PRIMER_LEFT_19_GC_PERCENT=36.842 PRIMER_RIGHT_19_GC_PERCENT=50.000 PRIMER_LEFT_19_SELF_ANY=5.00 PRIMER_RIGHT_19_SELF_ANY=7.00 PRIMER_LEFT_19_SELF_END=5.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_END_STABILITY=6.7000 PRIMER_RIGHT_19_END_STABILITY=6.1000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=1.00 PRIMER_PAIR_19_PRODUCT_SIZE=170 PRIMER_PAIR_20_PENALTY=13.720249 PRIMER_LEFT_20_PENALTY=10.882295 PRIMER_RIGHT_20_PENALTY=2.837954 PRIMER_LEFT_20_SEQUENCE=AGAAAGTGAAAGTAAATGTTATCCT PRIMER_RIGHT_20_SEQUENCE=CCAGGGGATTTGCAAGAG PRIMER_LEFT_20=9,25 PRIMER_RIGHT_20=180,18 PRIMER_LEFT_20_TM=54.118 PRIMER_RIGHT_20_TM=59.162 PRIMER_LEFT_20_GC_PERCENT=28.000 PRIMER_RIGHT_20_GC_PERCENT=55.556 PRIMER_LEFT_20_SELF_ANY=4.00 PRIMER_RIGHT_20_SELF_ANY=6.00 PRIMER_LEFT_20_SELF_END=2.00 PRIMER_RIGHT_20_SELF_END=2.00 PRIMER_LEFT_20_END_STABILITY=7.8000 PRIMER_RIGHT_20_END_STABILITY=6.7000 PRIMER_PAIR_20_COMPL_ANY=4.00 PRIMER_PAIR_20_COMPL_END=2.00 PRIMER_PAIR_20_PRODUCT_SIZE=172 PRIMER_PAIR_21_PENALTY=16.926020 PRIMER_LEFT_21_PENALTY=10.930833 PRIMER_RIGHT_21_PENALTY=5.995187 PRIMER_LEFT_21_SEQUENCE=TTTTAGATATGAATTAGCAAGTCATT PRIMER_RIGHT_21_SEQUENCE=TTAGTACCCAGGGGATTTG PRIMER_LEFT_21=33,26 PRIMER_RIGHT_21=187,19 PRIMER_LEFT_21_TM=55.069 PRIMER_RIGHT_21_TM=55.005 PRIMER_LEFT_21_GC_PERCENT=23.077 PRIMER_RIGHT_21_GC_PERCENT=47.368 PRIMER_LEFT_21_SELF_ANY=8.00 PRIMER_RIGHT_21_SELF_ANY=5.00 PRIMER_LEFT_21_SELF_END=6.00 PRIMER_RIGHT_21_SELF_END=1.00 PRIMER_LEFT_21_END_STABILITY=6.9000 PRIMER_RIGHT_21_END_STABILITY=7.2000 PRIMER_PAIR_21_COMPL_ANY=4.00 PRIMER_PAIR_21_COMPL_END=1.00 PRIMER_PAIR_21_PRODUCT_SIZE=155 PRIMER_PAIR_22_PENALTY=21.081334 PRIMER_LEFT_22_PENALTY=10.961407 PRIMER_RIGHT_22_PENALTY=10.119926 PRIMER_LEFT_22_SEQUENCE=AGAAAGTGAAAGTAAATGTTATCC PRIMER_RIGHT_22_SEQUENCE=TTAGTACCCAGGGGATTT PRIMER_LEFT_22=9,24 PRIMER_RIGHT_22=187,18 PRIMER_LEFT_22_TM=53.039 PRIMER_RIGHT_22_TM=51.880 PRIMER_LEFT_22_GC_PERCENT=29.167 PRIMER_RIGHT_22_GC_PERCENT=44.444 PRIMER_LEFT_22_SELF_ANY=4.00 PRIMER_RIGHT_22_SELF_ANY=5.00 PRIMER_LEFT_22_SELF_END=0.00 PRIMER_RIGHT_22_SELF_END=3.00 PRIMER_LEFT_22_END_STABILITY=7.1000 PRIMER_RIGHT_22_END_STABILITY=6.9000 PRIMER_PAIR_22_COMPL_ANY=4.00 PRIMER_PAIR_22_COMPL_END=2.00 PRIMER_PAIR_22_PRODUCT_SIZE=179 = PRIMER_MIN_THREE_PRIME_DISTANCE=2 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=10 PRIMER_RIGHT_NUM_RETURNED=10 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=10 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=8.114341 PRIMER_LEFT_1_PENALTY=7.398215 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_1=0,22 PRIMER_RIGHT_1=179,20 PRIMER_LEFT_1_TM=54.602 PRIMER_RIGHT_1_TM=59.284 PRIMER_LEFT_1_GC_PERCENT=36.364 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=180 PRIMER_PAIR_2_PENALTY=9.153949 PRIMER_LEFT_2_PENALTY=7.538496 PRIMER_RIGHT_2_PENALTY=1.615453 PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_2_SEQUENCE=GTACCCAGGGGATTTGCAAG PRIMER_LEFT_2=0,25 PRIMER_RIGHT_2=184,20 PRIMER_LEFT_2_TM=57.462 PRIMER_RIGHT_2_TM=61.615 PRIMER_LEFT_2_GC_PERCENT=32.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.0000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=185 PRIMER_PAIR_3_PENALTY=9.454063 PRIMER_LEFT_3_PENALTY=7.694441 PRIMER_RIGHT_3_PENALTY=1.759622 PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAA PRIMER_RIGHT_3_SEQUENCE=GGGGATTTGCAAGAGACTTT PRIMER_LEFT_3=0,20 PRIMER_RIGHT_3=177,20 PRIMER_LEFT_3_TM=52.306 PRIMER_RIGHT_3_TM=58.240 PRIMER_LEFT_3_GC_PERCENT=35.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=178 PRIMER_PAIR_4_PENALTY=10.148846 PRIMER_LEFT_4_PENALTY=8.086311 PRIMER_RIGHT_4_PENALTY=2.062535 PRIMER_LEFT_4_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_4_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_4=30,27 PRIMER_RIGHT_4=171,21 PRIMER_LEFT_4_TM=58.914 PRIMER_RIGHT_4_TM=58.937 PRIMER_LEFT_4_GC_PERCENT=29.630 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=142 PRIMER_PAIR_5_PENALTY=11.936750 PRIMER_LEFT_5_PENALTY=9.823274 PRIMER_RIGHT_5_PENALTY=2.113476 PRIMER_LEFT_5_SEQUENCE=TCCTTTTAGATATGAATTAGCAAG PRIMER_RIGHT_5_SEQUENCE=GGGATTTGCAAGAGACTTTGC PRIMER_LEFT_5=30,24 PRIMER_RIGHT_5=176,21 PRIMER_LEFT_5_TM=54.177 PRIMER_RIGHT_5_TM=61.113 PRIMER_LEFT_5_GC_PERCENT=29.167 PRIMER_RIGHT_5_GC_PERCENT=47.619 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=7.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=7.00 PRIMER_LEFT_5_END_STABILITY=8.5000 PRIMER_RIGHT_5_END_STABILITY=8.8000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PAIR_5_PRODUCT_SIZE=147 PRIMER_PAIR_6_PENALTY=12.157591 PRIMER_LEFT_6_PENALTY=10.026416 PRIMER_RIGHT_6_PENALTY=2.131175 PRIMER_LEFT_6_SEQUENCE=TGTTATCCTTTTAGATATGAATTAGCA PRIMER_RIGHT_6_SEQUENCE=CCAGGGGATTTGCAAGAGA PRIMER_LEFT_6=25,27 PRIMER_RIGHT_6=180,19 PRIMER_LEFT_6_TM=56.974 PRIMER_RIGHT_6_TM=61.131 PRIMER_LEFT_6_GC_PERCENT=25.926 PRIMER_RIGHT_6_GC_PERCENT=52.632 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_END_STABILITY=7.5000 PRIMER_RIGHT_6_END_STABILITY=6.4000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PAIR_6_PRODUCT_SIZE=156 PRIMER_PAIR_7_PENALTY=12.399903 PRIMER_LEFT_7_PENALTY=10.037140 PRIMER_RIGHT_7_PENALTY=2.362762 PRIMER_LEFT_7_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT PRIMER_RIGHT_7_SEQUENCE=GATTTGCAAGAGACTTTGCTCA PRIMER_LEFT_7=10,27 PRIMER_RIGHT_7=174,22 PRIMER_LEFT_7_TM=56.963 PRIMER_RIGHT_7_TM=59.637 PRIMER_LEFT_7_GC_PERCENT=25.926 PRIMER_RIGHT_7_GC_PERCENT=40.909 PRIMER_LEFT_7_SELF_ANY=6.00 PRIMER_RIGHT_7_SELF_ANY=7.00 PRIMER_LEFT_7_SELF_END=4.00 PRIMER_RIGHT_7_SELF_END=5.00 PRIMER_LEFT_7_END_STABILITY=7.3000 PRIMER_RIGHT_7_END_STABILITY=8.2000 PRIMER_PAIR_7_COMPL_ANY=5.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PAIR_7_PRODUCT_SIZE=165 PRIMER_PAIR_8_PENALTY=13.149777 PRIMER_LEFT_8_PENALTY=10.534347 PRIMER_RIGHT_8_PENALTY=2.615430 PRIMER_LEFT_8_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTT PRIMER_RIGHT_8_SEQUENCE=GCAAGAGACTTTGCTCAGACA PRIMER_LEFT_8=10,25 PRIMER_RIGHT_8=169,21 PRIMER_LEFT_8_TM=54.466 PRIMER_RIGHT_8_TM=58.385 PRIMER_LEFT_8_GC_PERCENT=28.000 PRIMER_RIGHT_8_GC_PERCENT=47.619 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=7.00 PRIMER_LEFT_8_SELF_END=4.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_END_STABILITY=8.2000 PRIMER_RIGHT_8_END_STABILITY=6.4000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PAIR_8_PRODUCT_SIZE=160 PRIMER_PAIR_9_PENALTY=16.926020 PRIMER_LEFT_9_PENALTY=10.930833 PRIMER_RIGHT_9_PENALTY=5.995187 PRIMER_LEFT_9_SEQUENCE=TTTTAGATATGAATTAGCAAGTCATT PRIMER_RIGHT_9_SEQUENCE=TTAGTACCCAGGGGATTTG PRIMER_LEFT_9=33,26 PRIMER_RIGHT_9=187,19 PRIMER_LEFT_9_TM=55.069 PRIMER_RIGHT_9_TM=55.005 PRIMER_LEFT_9_GC_PERCENT=23.077 PRIMER_RIGHT_9_GC_PERCENT=47.368 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=5.00 PRIMER_LEFT_9_SELF_END=6.00 PRIMER_RIGHT_9_SELF_END=1.00 PRIMER_LEFT_9_END_STABILITY=6.9000 PRIMER_RIGHT_9_END_STABILITY=7.2000 PRIMER_PAIR_9_COMPL_ANY=4.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=155 = PRIMER_MIN_THREE_PRIME_DISTANCE=3 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=8 PRIMER_RIGHT_NUM_RETURNED=8 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=8 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=8.114341 PRIMER_LEFT_1_PENALTY=7.398215 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_1=0,22 PRIMER_RIGHT_1=179,20 PRIMER_LEFT_1_TM=54.602 PRIMER_RIGHT_1_TM=59.284 PRIMER_LEFT_1_GC_PERCENT=36.364 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=180 PRIMER_PAIR_2_PENALTY=10.058608 PRIMER_LEFT_2_PENALTY=8.086311 PRIMER_RIGHT_2_PENALTY=1.972297 PRIMER_LEFT_2_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_2_SEQUENCE=GGGGATTTGCAAGAGACTTTG PRIMER_LEFT_2=30,27 PRIMER_RIGHT_2=177,21 PRIMER_LEFT_2_TM=58.914 PRIMER_RIGHT_2_TM=60.972 PRIMER_LEFT_2_GC_PERCENT=29.630 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.3000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=148 PRIMER_PAIR_3_PENALTY=11.885808 PRIMER_LEFT_3_PENALTY=9.823274 PRIMER_RIGHT_3_PENALTY=2.062535 PRIMER_LEFT_3_SEQUENCE=TCCTTTTAGATATGAATTAGCAAG PRIMER_RIGHT_3_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_3=30,24 PRIMER_RIGHT_3=171,21 PRIMER_LEFT_3_TM=54.177 PRIMER_RIGHT_3_TM=58.937 PRIMER_LEFT_3_GC_PERCENT=29.167 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=142 PRIMER_PAIR_4_PENALTY=12.417677 PRIMER_LEFT_4_PENALTY=10.037140 PRIMER_RIGHT_4_PENALTY=2.380537 PRIMER_LEFT_4_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT PRIMER_RIGHT_4_SEQUENCE=GGATTTGCAAGAGACTTTGCTC PRIMER_LEFT_4=10,27 PRIMER_RIGHT_4=175,22 PRIMER_LEFT_4_TM=56.963 PRIMER_RIGHT_4_TM=60.381 PRIMER_LEFT_4_GC_PERCENT=25.926 PRIMER_RIGHT_4_GC_PERCENT=45.455 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=166 PRIMER_PAIR_5_PENALTY=13.226111 PRIMER_LEFT_5_PENALTY=10.587869 PRIMER_RIGHT_5_PENALTY=2.638241 PRIMER_LEFT_5_SEQUENCE=GCCTTCATTAGAAAGTGAA PRIMER_RIGHT_5_SEQUENCE=TACCCAGGGGATTTGCAAGA PRIMER_LEFT_5=0,19 PRIMER_RIGHT_5=183,20 PRIMER_LEFT_5_TM=50.412 PRIMER_RIGHT_5_TM=62.638 PRIMER_LEFT_5_GC_PERCENT=36.842 PRIMER_RIGHT_5_GC_PERCENT=50.000 PRIMER_LEFT_5_SELF_ANY=5.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=5.00 PRIMER_RIGHT_5_SELF_END=4.00 PRIMER_LEFT_5_END_STABILITY=6.7000 PRIMER_RIGHT_5_END_STABILITY=7.0000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PAIR_5_PRODUCT_SIZE=184 PRIMER_PAIR_6_PENALTY=13.596920 PRIMER_LEFT_6_PENALTY=10.882295 PRIMER_RIGHT_6_PENALTY=2.714625 PRIMER_LEFT_6_SEQUENCE=AGAAAGTGAAAGTAAATGTTATCCT PRIMER_RIGHT_6_SEQUENCE=GCAAGAGACTTTGCTCAGACAC PRIMER_LEFT_6=9,25 PRIMER_RIGHT_6=169,22 PRIMER_LEFT_6_TM=54.118 PRIMER_RIGHT_6_TM=59.285 PRIMER_LEFT_6_GC_PERCENT=28.000 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=7.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_END_STABILITY=7.8000 PRIMER_RIGHT_6_END_STABILITY=6.1000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PAIR_6_PRODUCT_SIZE=161 PRIMER_PAIR_7_PENALTY=21.337518 PRIMER_LEFT_7_PENALTY=11.217591 PRIMER_RIGHT_7_PENALTY=10.119926 PRIMER_LEFT_7_SEQUENCE=TGAAAGTAAATGTTATCCTTTTAGA PRIMER_RIGHT_7_SEQUENCE=TTAGTACCCAGGGGATTT PRIMER_LEFT_7=15,25 PRIMER_RIGHT_7=187,18 PRIMER_LEFT_7_TM=53.782 PRIMER_RIGHT_7_TM=51.880 PRIMER_LEFT_7_GC_PERCENT=24.000 PRIMER_RIGHT_7_GC_PERCENT=44.444 PRIMER_LEFT_7_SELF_ANY=6.00 PRIMER_RIGHT_7_SELF_ANY=5.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_END_STABILITY=6.0000 PRIMER_RIGHT_7_END_STABILITY=6.9000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PAIR_7_PRODUCT_SIZE=173 = PRIMER_MIN_THREE_PRIME_DISTANCE=4 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=4 PRIMER_RIGHT_NUM_RETURNED=4 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=4 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=8.114341 PRIMER_LEFT_1_PENALTY=7.398215 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_1=0,22 PRIMER_RIGHT_1=179,20 PRIMER_LEFT_1_TM=54.602 PRIMER_RIGHT_1_TM=59.284 PRIMER_LEFT_1_GC_PERCENT=36.364 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=180 PRIMER_PAIR_2_PENALTY=10.148846 PRIMER_LEFT_2_PENALTY=8.086311 PRIMER_RIGHT_2_PENALTY=2.062535 PRIMER_LEFT_2_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_2_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_2=30,27 PRIMER_RIGHT_2=171,21 PRIMER_LEFT_2_TM=58.914 PRIMER_RIGHT_2_TM=58.937 PRIMER_LEFT_2_GC_PERCENT=29.630 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=142 PRIMER_PAIR_3_PENALTY=12.031683 PRIMER_LEFT_3_PENALTY=9.918207 PRIMER_RIGHT_3_PENALTY=2.113476 PRIMER_LEFT_3_SEQUENCE=TCCTTTTAGATATGAATTAGCAA PRIMER_RIGHT_3_SEQUENCE=GGGATTTGCAAGAGACTTTGC PRIMER_LEFT_3=30,23 PRIMER_RIGHT_3=176,21 PRIMER_LEFT_3_TM=53.082 PRIMER_RIGHT_3_TM=61.113 PRIMER_LEFT_3_GC_PERCENT=26.087 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=7.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=8.8000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=147 = PRIMER_MIN_THREE_PRIME_DISTANCE=5 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=8.114341 PRIMER_LEFT_1_PENALTY=7.398215 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_1=0,22 PRIMER_RIGHT_1=179,20 PRIMER_LEFT_1_TM=54.602 PRIMER_RIGHT_1_TM=59.284 PRIMER_LEFT_1_GC_PERCENT=36.364 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=180 PRIMER_PAIR_2_PENALTY=10.148846 PRIMER_LEFT_2_PENALTY=8.086311 PRIMER_RIGHT_2_PENALTY=2.062535 PRIMER_LEFT_2_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_2_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_2=30,27 PRIMER_RIGHT_2=171,21 PRIMER_LEFT_2_TM=58.914 PRIMER_RIGHT_2_TM=58.937 PRIMER_LEFT_2_GC_PERCENT=29.630 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=142 = PRIMER_MIN_THREE_PRIME_DISTANCE=6 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=8.124092 PRIMER_LEFT_1_PENALTY=7.407966 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAG PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_1=0,21 PRIMER_RIGHT_1=179,20 PRIMER_LEFT_1_TM=53.592 PRIMER_RIGHT_1_TM=59.284 PRIMER_LEFT_1_GC_PERCENT=38.095 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=180 PRIMER_PAIR_2_PENALTY=10.148846 PRIMER_LEFT_2_PENALTY=8.086311 PRIMER_RIGHT_2_PENALTY=2.062535 PRIMER_LEFT_2_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_2_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_2=30,27 PRIMER_RIGHT_2=171,21 PRIMER_LEFT_2_TM=58.914 PRIMER_RIGHT_2_TM=58.937 PRIMER_LEFT_2_GC_PERCENT=29.630 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=142 = PRIMER_MIN_THREE_PRIME_DISTANCE=7 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=8.410567 PRIMER_LEFT_1_PENALTY=7.694441 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAA PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT PRIMER_LEFT_1=0,20 PRIMER_RIGHT_1=179,20 PRIMER_LEFT_1_TM=52.306 PRIMER_RIGHT_1_TM=59.284 PRIMER_LEFT_1_GC_PERCENT=35.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=180 PRIMER_PAIR_2_PENALTY=10.148846 PRIMER_LEFT_2_PENALTY=8.086311 PRIMER_RIGHT_2_PENALTY=2.062535 PRIMER_LEFT_2_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_2_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_2=30,27 PRIMER_RIGHT_2=171,21 PRIMER_LEFT_2_TM=58.914 PRIMER_RIGHT_2_TM=58.937 PRIMER_LEFT_2_GC_PERCENT=29.630 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=142 = PRIMER_MIN_THREE_PRIME_DISTANCE=8 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=9.701705 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=1.615394 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACTT PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=179,21 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=60.615 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=150 PRIMER_PAIR_2_PENALTY=12.099675 PRIMER_LEFT_2_PENALTY=10.037140 PRIMER_RIGHT_2_PENALTY=2.062535 PRIMER_LEFT_2_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT PRIMER_RIGHT_2_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_2=10,27 PRIMER_RIGHT_2=171,21 PRIMER_LEFT_2_TM=56.963 PRIMER_RIGHT_2_TM=58.937 PRIMER_LEFT_2_GC_PERCENT=25.926 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=162 = PRIMER_MIN_THREE_PRIME_DISTANCE=9 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=9.845933 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=1.759622 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=GGGGATTTGCAAGAGACTTT PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=177,20 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=58.240 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=148 PRIMER_PAIR_2_PENALTY=12.652570 PRIMER_LEFT_2_PENALTY=10.037140 PRIMER_RIGHT_2_PENALTY=2.615430 PRIMER_LEFT_2_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT PRIMER_RIGHT_2_SEQUENCE=GCAAGAGACTTTGCTCAGACA PRIMER_LEFT_2=10,27 PRIMER_RIGHT_2=169,21 PRIMER_LEFT_2_TM=56.963 PRIMER_RIGHT_2_TM=58.385 PRIMER_LEFT_2_GC_PERCENT=25.926 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=160 = PRIMER_MIN_THREE_PRIME_DISTANCE=10 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=10.058608 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=1.972297 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=GGGGATTTGCAAGAGACTTTG PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=177,21 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=60.972 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.3000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=148 = PRIMER_MIN_THREE_PRIME_DISTANCE=11 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=10.148846 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=2.062535 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=171,21 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=58.937 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=142 = PRIMER_MIN_THREE_PRIME_DISTANCE=12 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=10.148846 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=2.062535 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=171,21 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=58.937 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=142 = PRIMER_MIN_THREE_PRIME_DISTANCE=13 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=10.148846 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=2.062535 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=171,21 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=58.937 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=142 = PRIMER_MIN_THREE_PRIME_DISTANCE=14 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=10.148846 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=2.062535 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=171,21 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=58.937 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=142 = PRIMER_MIN_THREE_PRIME_DISTANCE=15 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=10.148846 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=2.062535 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=171,21 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=58.937 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=142 = PRIMER_MIN_THREE_PRIME_DISTANCE=16 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=10.148846 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=2.062535 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=171,21 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=58.937 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=142 = PRIMER_MIN_THREE_PRIME_DISTANCE=17 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=10.283451 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=2.197140 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGAC PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=171,22 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=59.803 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=142 = PRIMER_MIN_THREE_PRIME_DISTANCE=18 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=10.701741 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=2.615430 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=GCAAGAGACTTTGCTCAGACA PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=169,21 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=58.385 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=140 = PRIMER_MIN_THREE_PRIME_DISTANCE=19 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 PRIMER_PAIR_1_PENALTY=10.800936 PRIMER_LEFT_1_PENALTY=8.086311 PRIMER_RIGHT_1_PENALTY=2.714625 PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA PRIMER_RIGHT_1_SEQUENCE=GCAAGAGACTTTGCTCAGACAC PRIMER_LEFT_1=30,27 PRIMER_RIGHT_1=169,22 PRIMER_LEFT_1_TM=58.914 PRIMER_RIGHT_1_TM=59.285 PRIMER_LEFT_1_GC_PERCENT=29.630 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=140 = PRIMER_MIN_THREE_PRIME_DISTANCE=20 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 = PRIMER_MIN_THREE_PRIME_DISTANCE=21 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 = PRIMER_MIN_THREE_PRIME_DISTANCE=22 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=7.340839 PRIMER_LEFT_0_PENALTY=7.018216 PRIMER_RIGHT_0_PENALTY=0.322623 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA PRIMER_LEFT_0=0,27 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.982 PRIMER_RIGHT_0_TM=60.323 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=187 = P3_COMMENT='should complain' PRIMER_MIN_THREE_PRIME_DISTANCE=-2 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA PRIMER_ERROR=Minimum 3' distance must be >= -1 (min_*_three_prime_distance) = primer3-2.3.6/test/primer_rat_input0000664000077300007730000042546112231156245016357 0ustar ioanaioanaPRIMER_OPT_SIZE=20 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000 PRIMER_MAX_GC=80.0 PRIMER_MAX_SIZE=25 PRIMER_MAX_TM=63.0 PRIMER_MIN_SIZE=18 PRIMER_MIN_GC=20 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=6 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=6 SEQUENCE_ID=01.RHAP33FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,315 SEQUENCE_TARGET=81,30 P3_COMMENT=CL is GCCTTCATTAGAAAGTGAAAGTAAA P3_COMMENT=CR is GGCAAAAGGACAGAGGATGA = SEQUENCE_ID=01.RHAP34FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT SEQUENCE_INCLUDED_REGION=19,296 SEQUENCE_TARGET=134,15 SEQUENCE_TARGET=213,16 SEQUENCE_TARGET=235,16 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=03.RHAP34FA3.seq SEQUENCE_TEMPLATE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT SEQUENCE_INCLUDED_REGION=0,278 SEQUENCE_TARGET=1,28 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=03.RHAP34FE3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA SEQUENCE_INCLUDED_REGION=17,284 SEQUENCE_TARGET=27,60 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=04.RHAP33FE4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC SEQUENCE_INCLUDED_REGION=18,367 SEQUENCE_TARGET=146,46 SEQUENCE_TARGET=199,22 SEQUENCE_TARGET=286,13 SEQUENCE_TARGET=300,15 P3_COMMENT=CL is TGAGAAGGGAGGAGTAAGTACTGG P3_COMMENT=CR is CCTATGCCTATACACCACACACA = SEQUENCE_ID=05.RHAP33FA5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA SEQUENCE_INCLUDED_REGION=10,369 SEQUENCE_TARGET=30,44 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=05.RHAP33FE5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,306 SEQUENCE_TARGET=176,34 P3_COMMENT=CL is CATGTTCGTGCAAACACTCA P3_COMMENT=CR is AGACCCTGTCTCCAAAAGCA = SEQUENCE_ID=05.RHAP34FA5.seq SEQUENCE_TEMPLATE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA SEQUENCE_INCLUDED_REGION=9,297 SEQUENCE_TARGET=125,36 SEQUENCE_TARGET=162,24 SEQUENCE_TARGET=191,16 SEQUENCE_TARGET=214,14 P3_COMMENT=CL is GCGGCCGACATCTACTACTA P3_COMMENT=CR is TCTCTCCCCTTACTACTCTGTCTAA = SEQUENCE_ID=06.RHAP34FE6.seq SEQUENCE_TEMPLATE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA SEQUENCE_INCLUDED_REGION=8,287 SEQUENCE_TARGET=61,68 P3_COMMENT=CL is CAACCCAAAAATACAGACGGA P3_COMMENT=CR is CCCTGTGTTACCCGTCTCAA = SEQUENCE_ID=07.RHAP34FE7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT SEQUENCE_INCLUDED_REGION=18,283 SEQUENCE_TARGET=115,29 SEQUENCE_TARGET=145,20 SEQUENCE_TARGET=166,24 P3_COMMENT=CL is TGGGTACAAACTGACAGGCA P3_COMMENT=CR is TGTGCACGCATGTAGAGGTT = SEQUENCE_ID=08.RHAP34FE8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG SEQUENCE_INCLUDED_REGION=20,281 SEQUENCE_TARGET=119,62 P3_COMMENT=CL is TTTCCCCTTAGGACCAAGGT P3_COMMENT=CR is CAGCTCTTCAAAGGACCTGG = SEQUENCE_ID=09.RHAP33FA9.seq SEQUENCE_TEMPLATE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG SEQUENCE_INCLUDED_REGION=0,371 SEQUENCE_TARGET=151,18 SEQUENCE_TARGET=192,53 SEQUENCE_TARGET=248,16 P3_COMMENT=CL is TGAGACATCCTGTTACCATGTAGG P3_COMMENT=CR is CTGTCCCTCCCTCCCTCC = SEQUENCE_ID=09.RHAP34FA9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT SEQUENCE_INCLUDED_REGION=20,280 SEQUENCE_TARGET=44,42 P3_COMMENT=CL is TGATCAGGATTTCAGTGCTAATAT P3_COMMENT=CR is TTCCTCACAGAGGGAACAGG = SEQUENCE_ID=10.RHAP34FA10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=12,236 SEQUENCE_TARGET=155,32 P3_COMMENT=CL is CCTGCAATGGGATGAATACA P3_COMMENT=CR is CAAGGACAGAACTGAACTGGC = SEQUENCE_ID=13.RHAP34FB1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,244 SEQUENCE_TARGET=22,16 SEQUENCE_TARGET=57,34 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=14.RHAP34FB2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC SEQUENCE_INCLUDED_REGION=13,303 SEQUENCE_TARGET=177,33 SEQUENCE_TARGET=254,14 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=14.RHAP34FF2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCATTTGGAAAGTCAACCTTCAAGGCGTGTGTTCTTCCTCCTTGGCACCTTGAGGAGGCAGCAATGACATTTTGACAAATGCCTCAAGTTAGGTAGGTGCTCTATAGCAAAGAGCCAATACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGTACGAATGTGTGTAATGAAAGGGGAATATTCTGGCTTAGCTTACACATTAAGTTCATTCAAGGTCATTCAATAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,248 SEQUENCE_TARGET=141,44 P3_COMMENT=CL is GGAGGCAGCAATGACATTTT P3_COMMENT=CR is TACACACATTCGTACACGCG = SEQUENCE_ID=15.RHAP34FF3.seq SEQUENCE_TEMPLATE=NNNNNNNNCACTGCCTGTCTCTGCTCTTTACTGAGAACAGTCAAGGGGACAGTATTGTTATCTCCACATATTTCACACTTTTCTTAACCCTTTCCTTAAGATTTTATCTCCTCCATTTTNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGGCCCNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTGCTGGGCTCTTAAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=8,193 SEQUENCE_TARGET=119,24 SEQUENCE_TARGET=154,25 P3_COMMENT=CL is TGAGAACAGTCAAGGGGACA P3_COMMENT=CR is CCACTTAAGAGCCCAGCAAA = SEQUENCE_ID=16.RHAP33FF4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCTTTGGACTCTGATGGAAGAACAGAATGAAAAGGATTATGGCCTCTAAGGGCAAAGGTACTCCACAGGCTTTGTCTTCATGTGGGTGTCGAACAACTAGAATTGGGCTTTCCCAAAACATGTTGTCTGTAGATGGGATATCTGTGTCTTTAAAATTCATTTATTTATTTACTCACTTTATATCCCAAAACTGGTGCTCCATCTTCATGGGATACCAACACCCATTGTTAGGGGAACCCTTAAAAAAACAAAGCGTGCGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGCAATGGGGGGTGAGGAGNCAGGAGGAGNNNNCNNAGGACAGAGGACCGAGT SEQUENCE_INCLUDED_REGION=17,363 SEQUENCE_TARGET=278,41 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=16.RHAP34FF4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCAGAAAACTCCGGAAGAGACCACCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAACCCATTCACGTGGGAGAGGGAGTGGACGGGCTCATTCTTGGATCTGCGGAGGGGTGGTTCGTAGGCACCTGGGAAGGGACTCTCCCATTTCTTACTGGTGTGGTGGGGTCTTGAAAGGAAGCTGCCCAAGCTGGGCGCTGGGCGGATGGCTCACGCCTTTAATTCCCCCGCACTGGGGGGGTAGCCAGTAGGAGAGATGGACCACCGANTTC SEQUENCE_INCLUDED_REGION=20,279 SEQUENCE_TARGET=46,38 P3_COMMENT=CL is AACTCCGGAAGAGACCACCT P3_COMMENT=CR is GAAATGGGAGAGTCCCTTCC = SEQUENCE_ID=17.RHAP34FF5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCTTGACTTCTATGTATGCACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATCTTATACATCTCTATTGTGTCCTTGTCATGTGGAGGTCAGAGATGAATATACATCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGTTGTGGTGNACATCTCTCTCTGTTTATATTGTGGCTTGTCATGGGGAGGCCAAGAGGTTTAAGGACTCACACAGCTCT SEQUENCE_INCLUDED_REGION=18,285 SEQUENCE_TARGET=40,50 SEQUENCE_TARGET=154,26 SEQUENCE_TARGET=188,33 P3_COMMENT=CL is TCCTTGTCATGTGGAGGTCA P3_COMMENT=CR is CTTAAACCTCTTGGCCTCCC = SEQUENCE_ID=18.RHAP34FB6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNACATGGTTATATGTCAAAATAATTTAATTTGTTGGTTGTTTCTTCCCAAAAATTACTTTAGTAAGAGTCCAAAAACTATTATTAATTAATATTAAGTTTTNGGNGNTGGTGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGTGATGTGTGCGTGATGTGAGCATGTTCCATGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=13,197 SEQUENCE_TARGET=127,46 P3_COMMENT=CL is TGTTGGTTGTTTCTTCCCAA P3_COMMENT=CR is CCATGGAACATGCTCACATC = SEQUENCE_ID=18.RHAP34FF6.seq SEQUENCE_TEMPLATE=NNNNNNNNNCCGTCTCAGTTAAAAGCAAATCTTGCCTCCATTCTGCGGGTTATTTTTGATGGTTTCCTCCTCATCTTCACTGGTCTGTTCTGGTGTTTGCTCCTAGGATTTGTCACGTGAATCCTGACTGATCCGACTTGCTTGTTTTTTTTTGCTCTTTCTTTAGTTCTCTATTTCTTTGCTCTTTTTCCTTGTACGGAAAGTTTTTTGCTTTCTCCTTTTTTCTTAAATGCACGCATGTGACTATTGTGATGGANNNNNNNNNNNNNNNNNNNNNNNNNATNNNN SEQUENCE_INCLUDED_REGION=9,274 SEQUENCE_TARGET=256,25 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=19.RHAP34FB7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTTTTCCACTTGTCTTGTTTCCAAACCTCCCTTTCCATCGAAAGCCATATAGTCATCTTTGTCCTATTATCAGCGNNNNNNNNNNNNNNNNNNNNANANANANGNGNGNGNGNGNANNNCNATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGGGGGNANAGAGNAATATGTGNATATGNGNANATANGGGGAAANACACANANANGGGGGNAGNGNGCGCGNAAGNGNNGNNAGAANCANGGGANNAGNAGAGTGNNNNGNGGGGGNAG SEQUENCE_INCLUDED_REGION=19,286 SEQUENCE_TARGET=93,20 SEQUENCE_TARGET=147,38 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=20.RHAP33FB8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTCTAGTAATCCTATTAGTTACACATGTAAATAACTAAGTGTAGCCCCTTAGGTGGAGACACCAGAGGGACAAGTGCATATTAGTTATTAGTAAACAAAAGAGCAAAAGGCTCTGAAGCTCTAGATCAACAAACAAAATCTATGGATGTAAAGAAATAATAAAGTCCAGGACAGAAGGAAATGAAACGTTGACAAAGTAATACCAATAATCAGTGAAACTAAAAGATGAGTGAGATTCACAGTTACTATTTGTAAGAGGTACATAGAATATTCGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATAGTGCCTTAAGTACATATATATTTTATAGAAATGNATTACTTTAGG SEQUENCE_INCLUDED_REGION=23,356 SEQUENCE_TARGET=299,30 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=20.RHAP34FB8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACAAAGTGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATACTGAGCTTCTAGAAGTAATATAGTTGCCAGAGANNANGGGGAAATATTGAGAACTTGCTAAATGTGTGCAGTCATAGTCAGACAAACAAAATAAGCTCTGGAGATCCATTACACAGATCGTGATTACAGCTCCCAATATTATTTCGAATAAGACACAGTAACTATACAAGGAAGCATTTGGATGTTCTTATACAGAAGAAATGATA SEQUENCE_INCLUDED_REGION=19,278 SEQUENCE_TARGET=30,56 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=20.RHAP34FF8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCAAATGCCAGCATGCAAAACTATAAAAGGATAAAAATGAAAATAGAAATGAAGTGTTGGCAGGAGCAGAAAGTGATGGCGCAGAATAGGAGGGCATTTCACAATAGTCCATGTGGCAAGAACTGTAGCCACTGATACATTTCTTTTTTAGTTCCTCTTTACAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCTTTAAAAATCTGCTTAAAAGAGCAGACAATATTAAGTTCCTG SEQUENCE_INCLUDED_REGION=20,279 SEQUENCE_TARGET=184,70 P3_COMMENT=CL is TGTGGCAAGAACTGTAGCCA P3_COMMENT=CR is TGTCTGCTCTTTTAAGCAGATTTTT = SEQUENCE_ID=21.RHAP33FB9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTCTTCATCCAGTCACCAACACGGGGCAGACTCAGGCTCATTGAGAAGCCTGCAGGTACAGAATGGCCCTGTTCCCTCTTACTAAATTTTGCTTGCAGTCAAGTTGCATAGCATGGAGTCCTGGTTGCAGGAATATCTCTATAGAGTTCTCAGATGCCCCCTCTCAGTGTTTCCATAAAACCTGGCTACCACGTGATGGAAGAAACAGCCTGATGTTACCATTTCCTTATTTTCACATGTATCTGCATGCATANNNNNNNNNNNNNNNNNNNNNNNNCTCTTGCTTGTAGAAGCTCACCAAAGCCATGCTTATACTACAGCCTGGTCTATTTCACATCCTTCAAGAGGTATACACAC SEQUENCE_INCLUDED_REGION=21,357 SEQUENCE_TARGET=274,24 P3_COMMENT=CL is AGTTCTCAGATGCCCCCTCT P3_COMMENT=CR is CATGGCTTTGGTGAGCTTCT = SEQUENCE_ID=21.RHAP33FF9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTATGAAGTAGCAGTGAAACTAAGTTTATGGCTGGGAGTCATTAGAACATGAGGAACCGGTTAAAGGGTGACAGCGTTAGGACTGAGAACCTGTGCTTTAGAGCAAGCCTCAACTGCCCACTTGCAGACAGCAGTTCTCCAGTCATTTTCAACTTGTTCATTTCCAATGGAGACAAGATATCTATTTATCACGTGCAGCATGATAAATAANNNNNNNNNNNNNNNNNACATCCCCAACAGTGAGGACCAGGCTCTACTTGAAAACCCATTTCCACACCCACTCTCACTCATGAACAGACATACACATAATGAATGTATTTTTGTGAAGCACCCATTTCATACCTACCAAGAAAAATAATTCATTTTCT SEQUENCE_INCLUDED_REGION=27,367 SEQUENCE_TARGET=236,17 P3_COMMENT=CL is CCTGTGCTTTAGAGCAAGCC P3_COMMENT=CR is GTCCTCACTGTTGGGGATGT = SEQUENCE_ID=21.RHAP34FB9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNGTAAAACATTCCTTCTTTCTAGCAATCATTCGTATACAAACTAAAACATAATCTGTGTCTTGCTTACAAGCATTTCAAATATTCAAAAATGTTATTTNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTAGTGTATGTCTATGTTTATATGTGTCCTTGTTTATATGTGTGTTCATGTTTATAGGTATGTGTCTATGTTTATATGTGTGTCCATGTTTATATGTGTGCCTGTTCATATGTGTGTTCATGNTTATAGGTGTGTGTCCATGTTTATAT SEQUENCE_INCLUDED_REGION=20,274 SEQUENCE_TARGET=117,26 P3_COMMENT=CL is TGTCTTGCTTACAAGCATTTCAA P3_COMMENT=CR is CAGGCACACATATAAACATGGA = SEQUENCE_ID=22.RHAP33FF10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCTGCCTCCCAGGCAGGATGGCCGCTCACGTTGAGGGATGGCTCCCTACAGAGGCAGCAGGGGTAATGGGGAGCTTATCTTACTTCATTCACTCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGANNNNNNNNNNNNNNNNCTTATAATTTTGGGAGTTTTTTTCTTTTCATTATCTTTGGCCTCCAAAGATTTTAAAGAGTCNAGCCCAACTTCTTGCAGGTCTTCAGGGAACGCCCCAGTAAATCAAGCTGCAGGCAATGGACANGGAGAATACACCTTCCTCAAGCNCCCGAAAGAAGAGCCAAAAGGNAGAAGCCCACTGANNCCCGC SEQUENCE_INCLUDED_REGION=18,370 SEQUENCE_TARGET=113,63 SEQUENCE_TARGET=181,16 P3_COMMENT=CL is GGGGTAATGGGGAGCTTATC P3_COMMENT=CR is TTGGAGGCCAAAGATAATGAA = SEQUENCE_ID=22.RHAP34FB10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACACNCNCCCATGGTGAATGTGTTTCGNGCTCTTTTTCNTGGCTAATCAAGNGTTACCCCNTGGGGACATGAATCATGGAGAAACACAATACNAGAGAAANNTCCCAAGTTACCANNAGACCCAGTCCANGAATAGANGAGTAANNNNNNNNNNNNNNNNNAAAATATTGAAAANCCACTCACATACACAGNCATCNTACNTAACAAGNCACATACACATNNCACNGAGACACCACATGACACATACNCATAAATNAACAGACATAGAGAGAGACATAAAGACATGGACNCAAAGAGAANTATCATACACACAANGAAAGTAAAAAAATTACCACATTCAAACNCACNCANNGAGGACAANCAATTGNCAATTAAGAAAAGNCATACAAAAAATNCAAATNGAGANNGAT SEQUENCE_INCLUDED_REGION=23,438 SEQUENCE_TARGET=23,27 SEQUENCE_TARGET=195,17 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=22.RHAP34FF10.seq SEQUENCE_TEMPLATE=NNNNNNNNNCCTCATATCCGGCATACATGCCTTCCCTGCCATCCTCAGCTGTATCCCCACAAACCTGAACTAAAATAAACCCTTCCCTGTTGCTTTTCTTAGACATTCTGTCACAGCAGTGAGAAAGGTAACTAAAGCAGAATCCTGGTTCCAGAAGTGGGTTGTCCTTGTGATAAACCCATCTCATGGCGCCTAGGCTTTTGCAATTGGTTTATGTGAAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGTGATGGTGGGATGNT SEQUENCE_INCLUDED_REGION=9,284 SEQUENCE_TARGET=223,50 P3_COMMENT=CL is GCAGAATCCTGGTTCCAGAA P3_COMMENT=CR is CATCCCACCATCACAACC = SEQUENCE_ID=23.RHAP34FF11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCTACCAATTGTTCTTTATTTACAGGGAGGTCTACTCTGCCTCCTCATCCAGGCTTTCTTTCATTCATCTCCTTAGCTGATTGCATTAGATGCACACATTCATACACACAGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTGCTTCCCTGCAGAGGTCAAGTCTGGGAATAATACAAAGACTTCTCTGTAGGTCCACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,240 SEQUENCE_TARGET=133,64 P3_COMMENT=CL is CCTCCTCATCCAGGCTTTCT P3_COMMENT=CR is AGACTTGACCTCTGCAGGGA = SEQUENCE_ID=24.RHAP33FB12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTTTTAGGTGTATAGGTTACATNAGCAAGGTTNCAGTTTAAACTTATTGGTTAGCATATTGACCTTTGAATCTGCTGGCTAGCAAGCATGGACAGATTATGACATGCCTTGGAACTCAATCTGGGACCAAAGGTTCAGGTGACTGTCAGCCAGTACTTNNNNNNNNNNNNNNNNNNNNNNNNNTNTNNNNNNNNNNNNNNTCTCTCNGCGTCNCNNCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTTTNTGTGTGTNTGTTTTTTTNNGNNTATGTNNGGCAGCNNTATNGGNATTTGGGNNTANCGCTTTNNGACNNAANNGNGNCCNNNAGTGNAC SEQUENCE_INCLUDED_REGION=23,356 SEQUENCE_TARGET=181,22 SEQUENCE_TARGET=209,14 SEQUENCE_TARGET=243,40 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=24.RHAP34FB12.seq SEQUENCE_TEMPLATE=NNNGGGAGNTAAGCATGGAATCAAATCTGACAATAACTAACTAACAATAGTTGNCCTACCAAGTTGNAACCAAGCAACCATTTTGCTGAAAAANTGCTNTCTTTTTTCCANTTGANNTGGTTTTGAGACNTCCNNTANGTCAAAAAAAAATCNAGTGAANCCANTANGAGANGNTAGANGAGAGATATCANTANTCNTAGAGAGNTACTATACAAATATACATANTAACAAAAATAAGAGATACATAATCATAGNTACATAGATACATACATAGNTAACACATAAAAATAGAAAATAGAAACAATATATAGAAAGAATATATAAATNNATATATATAGAGACACNNNNNNNNNNNNNNAAACAAATATNCNTAATAGATAGNAAAGACNGNCAGACAGACCCANNNCNNAACNNNCTNTCTAACTACAAAC SEQUENCE_INCLUDED_REGION=3,428 SEQUENCE_TARGET=344,14 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=26.RHAP33FC2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTCGGCACATTTTTAAAATAGATTCAATCATTCAAATTCAATCACAAATTTTCTACCCCATAATAATTTCTAATCATTTCACAGTGCACACACTCCTGCATCTAAATGCCTTCTTCCACATGTCAAAATAGAATTCATGTCTATTACCACACCTCTGAACTATCAAATCATTCAAAATGAAGATCATAAAATGAAGATGCATGGAAATGATGTGTAATGCCATAATAGGATAGCTACAAGAGTAACAGCAGGAGTAGCCAGTCCTTTGGAGAGTCATATTTAACCATGTGCTAAATAAGAGTTAGCAATATAATGCTGTAAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAC SEQUENCE_INCLUDED_REGION=23,361 SEQUENCE_TARGET=348,33 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=26.RHAP34FG2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTAGATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACCTGCTACTTAGAATCAGGAGCCACAGAACAGTGCAGTTCAGGAAACATCGGTTGACTTGATCATGAAGTCCTTAGAAGGGATGGAACCCACAGAACTGAAGATGTCCACAGAGAACNNTAGATAGACCATACTGAGGAACTCTGACATATCACTATCTACTGACATNTGATATATGAAGAGTATGACCACACAATCATATTATCATAGACNNTAATATATAGAAAAGNTTAGATNNCACAGAGATATATATCATGACTNTT SEQUENCE_INCLUDED_REGION=29,334 SEQUENCE_TARGET=43,54 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=27.RHAP33FC3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCTGCATTTTCTAGAAAGCATAACTAAAAGTACATGGGATCCTTGTGCGTGGCAGAGACATAATCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGAGAGAATANNNNNNNNNNNNNNNNNNNNAGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANAGAGCANNNNNNNNNNNNNNNAGACCTGANTGANNNNNNNNNNNNNNNNNNNNNNCCAANGCTGGACANAGTGANATCAGTNCCCCNNACATNAC SEQUENCE_INCLUDED_REGION=11,358 SEQUENCE_TARGET=77,58 SEQUENCE_TARGET=146,20 SEQUENCE_TARGET=171,38 SEQUENCE_TARGET=217,54 SEQUENCE_TARGET=281,14 SEQUENCE_TARGET=307,22 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=27.RHAP33FG3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNCTAGGCAATGGATGGACTGGCACGGAGGTCTAATGCACAGAGCCACACTGCTAGAGAAGTCTGACCTAAAAAGTCAGTCAAATTAATAAATGAGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGAGGGGCTATGGGAATCTAAGAAACACAGTTTAAAATTGCNTTTAAATTAAATTTTTAGTTAGTATACCAAATAGCATTTTATTATGACAGTTTTGTNTACNNNNNNNNNNNNNNNNNNNNNNNNCTATGTATGTATATATAATTGAANTTGGCCTNTTATTTGNCCCCCCANNNCCCCCCNCAAGGNATANA SEQUENCE_INCLUDED_REGION=16,365 SEQUENCE_TARGET=114,68 SEQUENCE_TARGET=289,24 P3_COMMENT=CL is GCACAGAGCCACACTGCTAG P3_COMMENT=CR is TAGATTCCCATAGCCCCTCC = SEQUENCE_ID=28.RHAP33FC4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCTGTCCAACAGCCTGGCTGGATAGGTGAGGTCCAGCCTCAATATGAGATCCTGCCTCAAAAAATAAAGCATGATAGAGGAAGACACATAGACAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCTCAAAATTCTCTATTCCTTTAAACATATTCTCAGTCATCAGTAAAAGAAAATTTCTATTTATTAATAAGTTAGTAGGAATTATTTTGTTGCTAGTAGATTTTGATACAGACTTGTGTTGTCCAGGTTAGTCTTAAACTGGTCTCACATATTTTACTGCAGTCTGAAGTTACAGGCCTGTGTCANTGTGNNTGGCTTACAGNATTTGCT SEQUENCE_INCLUDED_REGION=22,359 SEQUENCE_TARGET=117,50 P3_COMMENT=CL is GGTGAGGTCCAGCCTCAATA P3_COMMENT=CR is GGCCTGTAACTTCAGACTGCA = SEQUENCE_ID=28.RHAP34FC4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNACAATAATATATGAGTTGTTCCTTTAATCATGAATCATATTTCCTAGTATATTTTGAAACAATATTATTCATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATCTTCAAATGTACTGAATATTTCATCTATCACTTCTAATACTGAGGAAGCACCCAAGCACTCACCTTCAGAGAGGCAAAAGTGTTAGGGAAAGCACATTTCTTCTTCTTAAATACAT SEQUENCE_INCLUDED_REGION=12,274 SEQUENCE_TARGET=91,72 P3_COMMENT=CL is GAGTTGTTCCTTTAATCATGAATCA P3_COMMENT=CR is GCCTCTCTGAAGGTGAGTGC = SEQUENCE_ID=28.RHAP34FG4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCTGGTATCCTCCCTCTTTTGTCTTAGTTTAACCAGGAGAAACTCTCCCAAACTTTAACCTTATTAACAATTGCATGCAAAGTATGCCTGACTTAGGAATGTGGTGCAAAAGTCAGTCCCAAGGCGAGGAAATAACAGGTATTTTCTGAAGTCTTGTCTTAAGTCTGTTGTTGCAGCGGCCTTGAGTTTAATCCGTGGCACTGAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGGGGCAAACTGGAATCTCAGGAA SEQUENCE_INCLUDED_REGION=20,280 SEQUENCE_TARGET=233,42 P3_COMMENT=CL is CAAGGCGAGGAAATAACAGG P3_COMMENT=CR is TCCTGAGATTCCAGTTTGCC = SEQUENCE_ID=29.RHAP33FG5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCTAATGCAGCATTGATTTCTGAAGNNNNNNNNNNNNNNATTTGGGCTTCTGATAATTGTTTGGTTTGCCTATTGCCTTAGAATTAGTGTCGATTACGACCAACAGGAAAACTTCAAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTTTTCNCACATGGACCTNTTTGTTTGAAAGTCCCTGCNGCTGCTTTGGACTGTGTGGATANNGTGCTCCACCTAGNCAGTTTGTGTGTATGGCATGNATATGCANCACACACACCGTGGTGCNTGGNTGNCCTGTGNAGGANAANAGCAGGGATNTTANATT SEQUENCE_INCLUDED_REGION=14,371 SEQUENCE_TARGET=38,14 SEQUENCE_TARGET=133,87 P3_COMMENT=CL is CGACCAACAGGAAAACTTCAA P3_COMMENT=CR is ATCCACACAGTCCAAAGCAG = SEQUENCE_ID=30.RHAP34FC6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNACCCACACGGGCACATTCAGCCGCANANATGAGNCNCAGNCGCACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNACAGCAACTTCTCACCAAACNATCCAAAACCTACNGACTTGAGGGGCNGTCCTTGTCGTAGCACTAAANGCANANGGCAGCAGGTGGACACTGGTGGTGTGGGCACCTTGNGTNNGAGAGNCGGGGGNNANGAGGNCCNGGAGGGGNGNNACCGANGCNTCGGNAAGNTTCGNNAANNCANNGGNTCANNAGNCGNGGNNNNCCNNGGGGGGGGNGNA SEQUENCE_INCLUDED_REGION=12,314 SEQUENCE_TARGET=58,18 P3_COMMENT=CL is CCACACGGGCACATTCAG P3_COMMENT=CR is CACACCACCAGTGTCCACC = SEQUENCE_ID=32.RHAP33FG8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTAGATGACAGGGTGCCCGCCTCCTGTGGGCGCAGGTGTGAGTCCTTGTAAGGCAACCTTGGGTTCCTGGCTGGACCTCAGAGGTTTCCCTCCATTGAGTGTGAGAGGGACTGTGGTAGTGGGGAAGGAACTGGTGGGCAGTGGAGGGTTCGCTCTTTAGACAGAGTACTACATACATAGAGAGTAAAGCAAAGAGCCGTAAAGAGGTGTGTTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACTTGTGTGGGTACACACATGCATGCAGACCTGANGCCNTCNNCAATTACNTNC SEQUENCE_INCLUDED_REGION=29,362 SEQUENCE_TARGET=248,35 SEQUENCE_TARGET=288,48 P3_COMMENT=CL is AAGCAAAGAGCCGTAAAGAGG P3_COMMENT=CR is AGGTCTGCATGCATGTGTGT = SEQUENCE_ID=33.RHAP33FG9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCTAGTCCAGTCAAATGGATGATCACTAGGTGCAAAGGGGGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGANAACAGCNTCCACCTAGAGCACNNNNNNNNNNNNNNACNAAGNAAACACATTTTCATTCCACACTTACATCATGCACACACATGCNTGCNGTGCATGNNNNNNNNNNNNNNNNCCCCCCCCANATAACNATAAAACCATACAGCAAACATNNTGTACAACCACCACNATNNNNNNNNNNNNNNTTTGTNTTCCGNTGGGCCAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=15,312 SEQUENCE_TARGET=59,58 SEQUENCE_TARGET=145,14 SEQUENCE_TARGET=220,16 SEQUENCE_TARGET=292,14 P3_COMMENT=CL is GATCACTAGGTGCAAAGGGG P3_COMMENT=CR is GCATGTGTGTGCATGATGTAA = SEQUENCE_ID=34.RHAP33FG10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCTATTTTGATCTCCATATGTTTTATTTCTATATCTATTCCTAATAGTTTTTANNNNNNNNNNNNNNNNGCATGCANNNNNNNNNNNNNNNNNNNNNNNNTCTGCANNNNNNNNNNNNNNNNNNNNNNNNNNTTGCTTAAAGATCAAGAAAGATGAGTTGTACCTGAGAAAACTTGCTAAGTCCCCCACTAGTAGTGGATCGACAATTAGACATTTTCCCATCAAGGACTGAAGCGTCTTTCTTGCGGACAGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=17,254 SEQUENCE_TARGET=69,16 SEQUENCE_TARGET=92,24 SEQUENCE_TARGET=122,26 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=35.RHAP33FG11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTAGAGACCACTGCGTCTCAGGAAATAAGGTGAAAAGAAACTGAGGAAGACAGCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCACTCACACAAGTTTTCTCAATTCCAGGCTATTTCCCCTTAGCGATAAGTGTAAGTTCATTCAAGAGCAGATGCTATTTTTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=27,174 SEQUENCE_TARGET=83,30 P3_COMMENT=CL is ACCACTGCGTCTCAGGAAAT P3_COMMENT=CR is GCTAAGGGGAAATAGCCTGG = SEQUENCE_ID=36.RHAP33FG12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCTTCTGTTTAGATTGTGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGGTTTCACAGGCTGGCTATTCATGCTTCATGGTCTTCAGTTCTGGGATAACAGATATGGCCACAAGTGGCTTACTTTAAAGTTTTGACATTAACAAACATTTTTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=17,174 SEQUENCE_TARGET=37,44 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=36.RHAP34FG12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCAGGTTATGCAAGGCTGGTTGGGTGCAGGGCTAAGAGATGCTGAAAAAAAAAGTGTATCTACAATTAGATTATTTCATTTCATTTGCTGTGGAGAATCTACAGGTGTGTGAGTGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTTTGAACTACTCTTGGGATAACATGTGGAAGAGTCTGGGTGTGGAAAGCTGTCATTCGGGAAAGAAAAATAAGAGCACTGTGATCCCAGGTACAAACATTTTAGAGGCAGATTTTAGGGNNNN SEQUENCE_INCLUDED_REGION=19,276 SEQUENCE_TARGET=137,36 P3_COMMENT=CL is GCAGGGCTAAGAGATGCTGA P3_COMMENT=CR is CACACCCAGACTCTTCCACA = SEQUENCE_ID=37.RHAP33FD1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNCTTCACTTTCATACATGGAAGTCACTATCTCAGAGCAGATACCCTGGTCCTCTGTCCCTTACAATCTCTCCGTGCTATCTTTCAGAGATGTTCCCTAAGCCTTAGGTGTAGGGGTTGTGTTGAAGATGCACCAAGGATAAGGATTCAGTCAGTTGTCTGCGTTTTTTTTTACCAGTTCTGAGTTTCTCTGATACCCTTTATCTATATGCAGCAGCTGGCAGCAGTATGTACTGTTGGCAAACTGATAAAATGTTCTGGATCTGTAATGAAAGACACACATATAGATAAGTATATANACATNNNNNNNNNNNNNNNNAAGNGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNCA SEQUENCE_INCLUDED_REGION=10,361 SEQUENCE_TARGET=310,16 SEQUENCE_TARGET=333,33 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=37.RHAP33FH1.seq SEQUENCE_TEMPLATE=NNNNTCCCGGTACGGGCGCGCCCNCCCCCGNNNNNNNNNNNNNNNNNNNNNTGTGGNGCGCGTTTGTCGGNTTTGNGTCCAGAGGCTGTTGTTAGGTATGGAGGGNGTGGAGGNGGAGGGTGTTCTTGGTGCCAGTGTGTGTNCGCNGCTGTTGGCGTTGNNNTTTGNGGGNGTNTGANGACCAANTGGCCNACGGTGCTGTGACATCAACCAGCTGGCAAAGNAAGNAGGNGCNAAGAGCGCAGCCTTCCCANGCCNNCACNGCNNNACCTCCTGCNNNTCTCCTAACCCCNNNCNCCCNCTTCNGCCTNCNCCTGNNCCTCCCTCAAGGCCGCCNNCCCNNTGTCCNNCNNCCCGCCCNNCNGGAANAGCCCCTCCACCCCCNNCNGCCNCACCNNCAATCCTCCNCNTNAACCCNCTGCCNCCT SEQUENCE_INCLUDED_REGION=4,423 SEQUENCE_TARGET=30,21 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=37.RHAP34FD1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNACTACAAGGNNNNNNNNNNNNNNNNNNNATTCATATATACAGCACACGCATACAAATNNNNNNNNNNNNNNNNNNNNAACAAACATACTTACTTACACACCACACAGACCCACTACGAACTCACACAAACATTCTACATAGACACATCACATACATACCATGCAGTNNNNNNNNNNNNNNNNNNCTCATACCACACCCAATACACACACACACGTCTACTCTCTACTTCGTCNACTATCGACNACTACACACACNACNNCATCTATCTACNGCATTTACTTTACATACAAAGTTATACAGTAGNAGTAGAGTGNACCGATGACTCNGA SEQUENCE_INCLUDED_REGION=18,328 SEQUENCE_TARGET=27,19 SEQUENCE_TARGET=75,20 SEQUENCE_TARGET=184,18 P3_COMMENT=CL is ACTTACACACCACACAGACCC P3_COMMENT=CR is GACGTGTGTGTGTGTATTGGG = SEQUENCE_ID=37.RHAP34FH1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCTGCATCTCCATACTCCATTGATGTAGTGCTAGGGACAGAATTCAGGGCATCATGCATGCTACAAACTAAGCTACATCCCTAGCCCCACATGTGAAGTCTTTTGTAACTGATGTCTACTGATTTGAACCTAAGGAGGCTATAAGAGAAGAGAACCACTGAACTCCATGAGATTTAGAACAGGAAATGGAATGGAGACTATTATTTTTTCCACTGTTTCTCTGTTTCTCTGTGTTTCTCTTANNNNNNNNNNNNNNNNNNNNTCTGTCCTGTTTAAGTGTGGA SEQUENCE_INCLUDED_REGION=18,283 SEQUENCE_TARGET=260,20 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=38.RHAP34FH2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCTGGGTTCGGTCCCCAGCTCCGGGGGGAAAAAAAAAAAAGAATTGCAGGTCAGTGTCATACAGGTCTTGGTGGTCTGTTTTCTTTAGTCTGTTTTATTCCATGCTGTCACACAGTAAAGATGCAGTCTACTACTGAGTTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTATCCACATGCCATAGAATGTGTGTTGGAGGTCTGAAGAATAGCTTTCAGAGAGTTGGTTCATCATCTTTCATCATATTTTTTTTCCCCAGAAATTG SEQUENCE_INCLUDED_REGION=20,282 SEQUENCE_TARGET=162,40 P3_COMMENT=CL is TGCAGGTCAGTGTCATACAGG P3_COMMENT=CR is TCCAACACACATTCTATGGCA = SEQUENCE_ID=39.RHAP33FD3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTTTTTGGAACACAAGTGNNNNNNNNNNNNNNNNNNNNAATCCTTTTAAGCCTTTTTAAAGAAATGATTGACATTGTGAATTAGACAATTTTCAGTTATAAATTTTCAGCACACAGCACAGAAAGCCTAAATGTGCAATAAAATGCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,148 SEQUENCE_TARGET=39,20 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=39.RHAP33FH3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNTATTTCTCTGTCTTTTCCTTCTACGTGCTTTCTTTCTTTTTCCCATCTCCCTAAACTCAACCAGGATTTACAGGAGAGAGGGACTTTACCATCTNNNNNNNNNNNNNNNNNNNNCCCACTGCACTCAAGCGCTCGCACGGTGCCCCTCCTCACAGCCTGTGTGCTTTGCTCTCTTCATATATGATTGACAGGGAGAAAAAAAATGAGAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,211 SEQUENCE_TARGET=108,20 P3_COMMENT=CL is CCCATCTCCCTAAACTCAACC P3_COMMENT=CR is GAGAGCAAAGCACACAGGCT = SEQUENCE_ID=39.RHAP34FH3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCTGTATGGAATAAAGTTTAAACATTTTTAAGTGTAACATAATAGTAAGCATACGACTGAAATGAATGTGATAGAGAAGGAAGTGGTTTGCCCAGCAAGGAATGTCATGACATCATAAAGTTATTCTGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGATTATATACATGCATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTCTGCTTCTTTTATTTCACATACAAAGGCCGTACTCTTGGAACTGTGAGTCAAAATAAGGACTTTGNCCNTG SEQUENCE_INCLUDED_REGION=18,286 SEQUENCE_TARGET=148,32 SEQUENCE_TARGET=202,28 P3_COMMENT=CL is CCCAGCAAGGAATGTCATG P3_COMMENT=CR is TTCCAAGAGTACGGCCTTTG = SEQUENCE_ID=40.RHAP33FD4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTCCGAAAAAAAGAAAAGAAAAAAAATTTAGTGATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCACAGATATCAGATTTTAATATGTGCTCTTCTTACTCACACTCTTCCACCTACCTTTCCTCCCTGGCGAACTTCTGCCCGGCTACTCTCTCCTCATTCCCACCCCAGATAATAGGTCTTTCCTTGCCTCTGTGCCTCTATGGCAATATGAATCTAGTTCTATTCTAACATGCTTTTGTTGATAATCATGCTTTGGGGGTATCTTCCATCCTGTCCACAAGCCTTTTCCCATAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,305 SEQUENCE_TARGET=61,32 P3_COMMENT=CL is TCCGAAAAAAAGAAAAGAAAAAAA P3_COMMENT=CR is AGGGAGGAAAGGTAGGTGGA = SEQUENCE_ID=40.RHAP33FH4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCTCCCACCTTCCTCATGTTGCCTTGCTCCAGTGGACAGCCAATTATGACAGATGATGGTGGAGGGACAAGATGTGCCCATATCCCCTCTCCCTTCATATCTTCTACTTCAGCAAGGGAGAGGCTAGCAGAGGGGGTTGAGTGTGTGTTCAGTAAGAAGGGACAGAGTAGTGCTAATGTGGGTGGCTTTCCCCACTGTTCTGGGGAAGACTGAANNNNNNNNNNNNNNNNNNNNNNTACAGAACTCAAAGTCTTTAATTCTGTGATGCATGGTAGAATTCATAGTAATGATAGAAAAATGGTTATTTTATTTTCTGTTCTAGNANGTNGGAAGGCATCGTGAACNAGTTTGNAGGAAAAAAGANCATT SEQUENCE_INCLUDED_REGION=15,367 SEQUENCE_TARGET=228,22 P3_COMMENT=CL is AAGGGAGAGGCTAGCAGAGG P3_COMMENT=CR is TGAATTCTACCATGCATCACAG = SEQUENCE_ID=40.RHAP34FH4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCAACTGAGGCTTCTACTGTGACTGCATTAAAGCTCAGCTTGTTCCCTCTGCTTCCATGGGTTTTTATTCCCCCAAAGCAACATTTGCTATACCTACCTCTGGTCTTTAACTGGTATCAGCAGTCTCAGAACAGCAACCCGAGGTTAGGGTGTTACGCCTAGCTAGCCCCGCTGGCAGTAAAGAACCCACGACAGTGTGGGTGGGGCCAGCTATTTCCAAATGCCATGTGGTACAGAAGGTAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,283 SEQUENCE_TARGET=264,38 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=41.RHAP33FD5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCTACAGTGTTTGGAGTTTGTCCTGACAGGTTTCTATTTTGCTCTGATGTAATATTTATTCATTATATCCCCTTTGCTGCCTTTATAACAGTTATGTGTATTCTGTGCCATCATCTGGTAGAAGTATGTCATTTGCTTTTTTAATTTTGATTTTACAGGGTATTATGATTAAAAGCCTGGAGTATCAGAAGACTCTGGACTTTTTAAACTGTACTGGATCAAATGCCTTTTGTNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=22,248 SEQUENCE_TARGET=254,15 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=41.RHAP33FH5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNTGACCAATCAGCATGTTTTATGTTAAGTTGAAAAGACACTGTGTTGTATGGATTACAGATTTGCTAGGTGGTTAAATATTCTTGGATTTTGGTAACACAGAGGCACATGTGCACGTGCACACATATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGATGGGGGGTGTTAATGGGGGAGGGAGAAAGNAGGAAGCAAGAAGCNATCCCTAACTGTAGTGAGGCAGGTAATGNAGAAAAGTGTAACAGATACCGACCTTTAGACAAGACTGCAGATATTAGANAAGCGATGGCTCTGTAATATAAATTTGTTAAGACATAGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,343 SEQUENCE_TARGET=142,42 P3_COMMENT=CL is CTTGGATTTTGGTAACACAGAGG P3_COMMENT=CR is CTTTCTCCCTCCCCCATTAA = SEQUENCE_ID=41.RHAP34FD5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNATATATTCTATGTATCTATGCNNNNNNNNNNNNNNNNNNNNAATTTATTTATTATTTTACCTTTGGGAAATACTCTCTTTGCAAAGCTTATAATGACTCCATGATAATTAAAAGCAGCAGGGGTTCAAGAGGCAAGAATGCATCTAGGTCTTAGCAAAATGGCAAATGCTGAGACCTTCAAGACCGTCATTAAGGCTGTGGGAATGAACTCTGAAAACATGTAGTTCAAATATAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,236 SEQUENCE_TARGET=40,20 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=41.RHAP34FH5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCAGGCATCTCTGAGGGAAAGCAGGAGAAGGAGTCTAATTCCTCTGCAAACTAATTAATGAGCCTTCCCAGAGTGCTGGCAGCTAACAGGCAAAACTGAGAACCACTAACAGCAAGTACACCAAAGCCAGTAAGTAGCAATGCTTTCCAGGTGTGTGCATGTATACCNNNNNNNNNNNNNNNNNNNNNNNNNNCTAGAGCACCCATGCATGTCATAGTGTGCATGGAGGTCAGAGGACAGCCCTGCCTTCTGCTCTTTGCCTTTCACTCTGTTTCAGACAGAC SEQUENCE_INCLUDED_REGION=18,282 SEQUENCE_TARGET=184,26 P3_COMMENT=CL is CAGCAAGTACACCAAAGCCA P3_COMMENT=CR is GTCCTCTGACCTCCATGCAC = SEQUENCE_ID=43.RHAP34FD7.seq SEQUENCE_TEMPLATE=ANACCCCACCNCCAGCNCCNCACACCNCAACCAGAACNGGTCNNNCNNTNTNNGTGCNNNNNNNNNNNNNNNNATCNCNCNGTGTGTGCCCNGCTCNGTATCNGTCNGNGTGTCNNGCCTCGCNNNNNNNNNNNNNNNNNNNNNNNNNCTGCGTGTCTCGTCGTGTGTGATTGATGTTGTGNGTCNTGTCGTGATGCTGNTCGTCTCTGATGTGNTNNGNNGNNTGNTGTTGNTAGNTNNTAGTGNNGNTANNGNTNCNNGCTCNTGNTNNNGNTCCNNGNTNNTCANNGANGNNGATNCTCGCTCNTNGNTGNTGATGNNGCACCNGNTGTNGCNGATA SEQUENCE_INCLUDED_REGION=0,340 SEQUENCE_TARGET=59,14 SEQUENCE_TARGET=124,24 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=44.RHAP33FD8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNTGCCATATTTCCCNAGCCATTAACTCATCTGACATAGGCCTTCTTCACGTTGCATCTAGATAGGTCTTCCTGTCTTCCTCTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGAGAGAGAGAAGGAGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,179 SEQUENCE_TARGET=95,56 SEQUENCE_TARGET=174,16 P3_COMMENT=CL is GCCTTCTTCACGTTGCATCT P3_COMMENT=CR is CCCCCTCCTTCTCTCTCTCT = SEQUENCE_ID=44.RHAP34FD8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNACTCCAAATAGTGCCTACATCCCATATTATTCTATCTTTGCTATCAATTGATTTTTTTCACAAAGTTTCTCTTCCTCCCAAATTAGAATGATTTCTCTCCCTTGTTTGGTTGTATGTTCATTCTTAAGCTGTAGTTAACACTGTGCCTTAGAACATCTTATTACAAGATCCCATTTAGCATTTACATAACCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAGCCATGGAGCACTATTATTTGTTGGTAATTGNTTAAATGCAAAAAACAATGTCCCTTG SEQUENCE_INCLUDED_REGION=11,284 SEQUENCE_TARGET=204,30 P3_COMMENT=CL is TTTCTCTCCCTTGTTTGGTTG P3_COMMENT=CR is CAAATAATAGTGCTCCATGGCTT = SEQUENCE_ID=45.RHAP33FD9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCTCGTTATGAAAAATTACATCTAATTGGAAGGTAGTTGAGTGAGTGGAGTGAAGTTGAGTTCAGTTCATTTCAGTGAGTCAGTGCAGTGGAATTGTGTTCATGCAATTCAGTGTAGGTCAGCTGGAGGCCCTTGTCCAGGCCACATGTAATACAGAAACAAAAATAGGGAATAGTACCAAGGAACTCTCACTAGAATAAAAGCAAGGAGTGTCCGCAGCACTGTGTTTATTCCTAATGNNNNNNNNNNNNNNNNGCCTCCCAAGGAGAGTCTGCCATGACCCCAGGTAAGAACTCACGCTGCATATGCCAATCCTTTGCTAGAGCATGTGAGCACCACGCCCACTCGACACAAACTTT SEQUENCE_INCLUDED_REGION=11,358 SEQUENCE_TARGET=249,16 P3_COMMENT=CL is TAAAAGCAAGGAGTGTCCGC P3_COMMENT=CR is AAAGTTTGTGTCGAGTGGGC = SEQUENCE_ID=46.RHAP33FH10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNACCTGAGAAATTACCTTGGGTTTGGTAGAACCAGGCAGGAATGAATGATGAAGAAATAAGTGAATAGGTCTTCAGCAGAGGGATTTCTGACCTTCTAGCTATTCATCACTTCTCATAAGTATCTAAGCTTTATAAGTGAGTTTTATCCAGTATGTCATGAGCTCGTGTTTATTTATATGTATTTGGATAAGCGTAAATACAAATGAACACTAATAAAAATACTTGTTAAAAATAGAAATCAAACAAATACACAAAGCAAAGAGATATNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNCGTTTTTATATGANNNCAACCNACCNNGGNAGAAANAANGTNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=26,355 SEQUENCE_TARGET=293,27 SEQUENCE_TARGET=321,19 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=46.RHAP34FD10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCTGCTGGATCTGTTTAGTGTTGTTCACGTGCATATGTTTCTAGGCCTAACCACTTACTAGGTATTGGATAATGGATTAGGGCTCAAACCTGGGATGACTGATACTCCTTTTCTCAATAGTTGGATAATGGATTAGGAGCTCAAACCTGGAGAAGACTAATACTCCTTCTCTCAGTAGTTGGATAATAGATTAGGGGCTCAAACCTGGAGAAGACTAATACTCC SEQUENCE_INCLUDED_REGION=0,281 SEQUENCE_TARGET=0,56 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=47.RHAP33FD11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCTGTTTTNGACCTCTTCCTCCTGGAAGGTCAAACTCTAAAGAGGGAACTATAGGGAAGAGTTAGTGGGGCCTTTAAATTGAGCAGCAACCACAAACTCAGTGGGTAGGAAAAAGAGAACAACAAGCTAGCAGGGTCTTTGTTCACCTAGATTCTTTCATATTCTGGGTTTTAAAGAAGTTACTATACATTAACTGTCCTGAAGATGTCCTTAGATAAGTTCCTCGCCCACTTGCCCCACTTCTGTATCCATCCACATTTAAATTCTCTAAAAGGCCATGAATCTTACTTCATTCCATGACTTTTCCCCTAGGGAAATNNNNNNNNNNNNNNNNTGTATGTATGNNNNNNNNNNNNNNNT SEQUENCE_INCLUDED_REGION=11,359 SEQUENCE_TARGET=328,16 SEQUENCE_TARGET=354,15 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=48.RHAP33FD12.seq SEQUENCE_TEMPLATE=CNTTTTANGGGGTTGGTGGTTCATCTGAAAGTGTTGAGCACCTTTTCTACTCCTGCTTATATATTTTTATCAAAAGAAGAAGAAGAAGGAGAAGAAAAATGAAATAGCAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGANTGATGTGTTTGTACGGNNAGNATGAGNTTTCATNGTTCTCTTNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGAAGGATGCTNNTCCTATACANTGAGAGAATATCCTTTATTACNCTGNCNTTAGTTTGTTACATGGAAGAATTTTTGCCTGCNGGTTTAGCCCTCAGGCAAGNCGNG SEQUENCE_INCLUDED_REGION=0,361 SEQUENCE_TARGET=112,58 SEQUENCE_TARGET=222,28 P3_COMMENT=CL is GGGTTGGTGGTTCATCTGAA P3_COMMENT=CR is GCAGGCAAAAATTCTTCCAT = SEQUENCE_ID=48.RHAP34FD12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNACCTAGGTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACAACTGTTAACGTAGGAAGCTTCGGTGATCTTCCTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=10,120 SEQUENCE_TARGET=21,33 SEQUENCE_TARGET=57,32 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=48.RHAP34FH12.seq SEQUENCE_TEMPLATE=CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTACAANAAGTCAGGGAGNGGGAGGAGTTCTGNACAGATGGCTTACTGAACCACAATGTGAAAAAGCATGACTTAATGGTGNTCATTTGAGTTTATCCCTTNTCAATACCAACGANAGGGAAGAANTTNAATTGACCAGACACACCGACNNTGAGGGGTCNNAATTGNAACACCNTAGAGAGAGACAAAGAAATGATACTNGNACAAGNTNTGNAAGAGAGNCACTATGTATGNAAGAAGTGNGAGAGNANACATTTCTCCNNGNCACGGTAATAAGCTNGAGAGAGNTNTANTCAACNAAGGAATACATNGGACATAAAAGNGNAAGAAAAGAATCNCATNGTCAACATTGNANTACACAATACACTGNAAGNAGCAAAGNTGA SEQUENCE_INCLUDED_REGION=0,420 SEQUENCE_TARGET=1,34 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=03.RHAP61FA3.seq SEQUENCE_TEMPLATE=GTCATTGTATTNATCACTACANCAATAAAAANGAAANNGCGACAACTCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTNTGTATGTCNNTTTANNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNTNGNGTGTGNCCCCTGTATATATGTGCTTNNNNGTGTGANNGCNCGNNTATNNNTNTNNGATNCCNGNTGGNNNNNNNNNNNNNNNNGTCGTNNANNNANNNNNTGNNANNTNAAGGGNNTCNACCCCNANANCANNTCTNTAGGCNCCANNGAANNTNGAAATNNNNNANNNANNGTCATTANCCNGTNNNCTGNCTCCAAANNNNTANCCGNNCAANANNNCCCANAATCANNNCAAGNCNGAANTANNNANCCGCAAANNNNCNGGNNNCNNAANNNTGTCNNNNNNANNCACNNTTANNNCGCNCCCNCNNNTCACCNGCCAGNNTTGCANCNNNGNANAT SEQUENCE_INCLUDED_REGION=0,485 SEQUENCE_TARGET=52,30 SEQUENCE_TARGET=102,16 SEQUENCE_TARGET=119,20 SEQUENCE_TARGET=213,14 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=04.RHAP61FB1.seq SEQUENCE_TEMPLATE=AGACCCCCTTGTTGATGTGTTTGTGCACAGATCNGTATCTGTGAACACTGACAGAAGGGAGGAAGGAACTGAGTGTCTTGAAAGATATGAGGGAATATGGCTATCTCTGTNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNTNNNNTTTTATATACATCTGCATCTTTNCATAGACACCCCATGAAAANGNACCTTGCAAATTCNTTATGGGNNANGTNTGNGTTTGTCAAAATNGGANTNNTCTTGNNAGCTAACTTCCGGNTNNNTAGAATGTATTATCCNCTNTATGTTNGACACTCTNTTGTTGNTGTGTTTCNTAAATAGGGGTANGCGCTCTGNNTTCTGTANNCNTNGGTCNTAGCTGTTTCCTGTTGGNAAATTTNTTNTGCGCNCNTATATTCCNCNNNACATTGTGNGGCGCANNCNTANTGTTTAAACNCNNGGGTCCCNNGTGAGGGCGCGTTTCGCNCNT SEQUENCE_INCLUDED_REGION=0,538 SEQUENCE_TARGET=113,34 SEQUENCE_TARGET=148,54 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=04.RHAP61FB7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNTGTAGTGTAAGTACCANTTATAACCCTAAGCCAGNTTTGAGAGGCAATAAGTCAACACTTGGATGAGAAAGTGGNACCAGGCCTTTGGTTTGGTCAACAACCTTTTGAGGCAACCATATTATCACCCNTTATTTCACAGATGAAGAAGTTGCACAAAGAAAGTAAAGCAGTATCATATATACACACACATATATGTATACCTACCCATCATATATACATACATATGTTACATATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCGTAAGCTCCNNAGCCNGGCCTGCANNTGGGGGGATCCNNTGNCAGTGCACCGCACANTGTATCACTGCGNGAAGATCCGNCAGGGNNTCTAGTTTCNNCAACNGCNTNNCNGAGGGATGCNGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,401 SEQUENCE_TARGET=257,36 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=06.RHAP61FB3.seq SEQUENCE_TEMPLATE=TCAAGATGGTGCAGAGGTAAAGGCAGTTGCTGCCAAGATTGAANACCTGACTTCAATCACTNNAACCTACATGTTGGAGGGAANAACGNACTCCAATTTTTCCTCTGACCTTCAACAGGCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNTGCTTAAATTCAAACANTGANATTGTNATTGGCCAAAATGNTTNATTTCCGCTCACANTTTTANAGCATTCNGGGGTCGGNGGGCNTCCTCCNTNGGNATTTGNNCCNGGGNNANCNACNTTGTCGANAATAAGAACCATGNNCGCGCCAANGANGNNNANNGAGGGTTNCNNGCTCGNNTTNGTANNNNANGGTCANANNNGTTTCNTGGGNNNANNTGGTNTCNGGNNNNNAAATCNNNNCAANNTTCNNGGCCNTGGAGGNATTAAGGNGNNNGCCTTGGGGGGGNNANNGNGTNAGT SEQUENCE_INCLUDED_REGION=0,528 SEQUENCE_TARGET=124,55 SEQUENCE_TARGET=181,40 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=06.RHAP61FB9.seq SEQUENCE_TEMPLATE=CNTCCNCCNNCCNCCNCTCNCGCCNNNNNNNNNNNNNNNNNNCNNNNNCNNNNNNNNNNNCNNNCNGANTNTGGTGNAATNTGNNGNTCNCNTNGGGGATCNNNAANCNCCCNNANNNNNNNNNNGNNGNNGANNNGTNCNANNTTNNNNNTTGGNGNTNAANNNNNAAATNNNTGNTNNGNAGNANGNGGNNTAAGNNNNCNNTANANGANNNNCNNNANNNNGNNNNNAGTANNNNATNGGNNNNGTTTNGGANGNNGNNATNGNNNAGANNAANNGNGNTGNGACTAGGGNGATNNGGANAAGANGGNNANNGNAAGNGGNGTNNNGGNNTNNTGGNANNTGTNNTGNNNGANNGGNNGNGGNNTAGGANNGNNTGTNNNTNTGGTNNANAAGTNNGNNNTGNNNNTGNNNNNNGGGTTTNNGNANGNNNNNNNNNTTAANNGGNNNNNNANANNTANNTGNNAAAAGGNNNNNNGGGANNNGNNNTAAGNANNGGGNGTNNGGNNGNGGNNNNNTGTGNNNGNNGNTTGGNGNNTNGNAGNNNNGNNNNNNNANGNNNTGNNNAAANT SEQUENCE_INCLUDED_REGION=0,572 SEQUENCE_TARGET=24,16 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=07.RHAP61FC7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNTGGGCGTGGTCCCGAGACACTCCCCGCGGGGGCGNCCGGGGCGGGGCGCGGCACCAGGTGGAGGGCGCTGACCCAAGCAACGTCAGTCTGGAAACTGCGTTCCCTAGGCATCTTATCCCCCTAGACCTAGCAGCAGAAGTACCCCAATTCAGTCTCTCAATTCAGTGCTCTTGGAGGAGAGTGTACCCACTCAAGGANTNNCNCGGGCTGGCTGAGCGGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,267 SEQUENCE_TARGET=233,44 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=11.RHAP61FD8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTCAGAATTGTTGGGAAGACAGAGTGAAACCCATGGCCTGAGCAGTATCTAGTCTCGGTAANTAGCAAGAAGANAAGGCTAGAAAGGCTGAGGTCTAGATAGGGCTATCCTGGGCTAGACTGAGTGGGTGATCATGACCAACACCAAGGGACTCAGGTGAAGCCCTCTACAAAGGTCCAGACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGNGNGNNNNNNNNNNNNNNNGTCGTGNTGNGGTGTNNGNGTNNGNNNNNNNGGGTGTGNNGTGNNNNNNNGNNTNGGGGNNGNNNNNNNNGGNNNGNNGANNNAGNGGGNGGAGGNCTNTAGNGNANGNNGNNNNNNGGNNANAGNNNNNNNNNGGGTNNNNGNNGNNNANNNNGNNGGNNNANNGTGGGGTNNNGGGGNNAGNNGNNGGNNTNGNGNGAAGNGNNGGGGNNNTGNGNNANNGGGNTNGANNNGNGNGNNNGNGGNA SEQUENCE_INCLUDED_REGION=19,483 SEQUENCE_TARGET=203,28 SEQUENCE_TARGET=240,15 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=12.RHAP61FD9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTGTTTTGGAAATTNGAGGAACAAAATATAAAANNCATTAAAAAATTCCCATTTCTGAAAATTATCACTGGAGTTANGGACAATTATGAGCCACACATTGTGGCTCATGATAAGAATNNNCAGAGGGAAANTANGATCTNNNTGNGAAAGAGGGACTNTGNAGNGNCNCNCNNANNGNCNNNAANNNNNCGNTNNGNNNCCNNCTCNNTCACTGNNNNNNNNNNNNNNNNCTNNTCNNTTTNTAGAATCGNGNNGNTGGTCTGAAAACNTNNNNGNNCACNNNNTCTTGGTTANCNTCANTTNTAAAATGNNTGANGNTGNTAGTAGGGNNGCNGCGTGNNNNCCNCCNTCCCAGGNNANCCNGNGCCCCATGNNNCNTCAAANCAANTNNNTGGANCCNCAANGTGNNANGGCCCNNCNATCCNNGNNNNTCNTGNNNNNGNGNTCATTGCAAANTNAGNGNNNNNNNNCNNNANTG SEQUENCE_INCLUDED_REGION=21,478 SEQUENCE_TARGET=236,14 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=13.RHAP61FE7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTTGAACATTTACTACATTTCCTGGGCTCACCANTTTGTNAATTTGCCTTTTTCTGTTCCATATGCANTTTTACCATCATCAGGTATAATGAATGGGTCTTAGCAGATTCCACTTGGGTGNTCTANTGAATTCTNNNNNNNNNNNNNNNNNNNNNNNNNGNGNNNCCNCCCNCNNNNNCNNTGNNNNNNNNNNNTGNNNNNGNCNCNNNNNCGGNNNGNGGGNNGCTGNGGAANNNNGGNNGNGNNGGGGNGNNNNGGGGNNNGNNNNGGGTGGGGGNGNNGCAGTGNNNNGGGGNNGNGGNGNGNNTGNGGNGTGGNNGNNNGGNNGTGNNNGGGNGGNGGGGGTTGGGNGNNGNNGTNNNAANNGGGGGNNNNGTNGGGGGNNGNNNNGNNNNNNNNNGGGANNNGGGGGNNTTNNNNGGGNGAGNNNGANNNGNNNNNGGGNNNANNNNNGAGGGNNGGNANNNGTGNGGGNNAGGGNNNGTTNNGGNNTTAAA SEQUENCE_INCLUDED_REGION=21,497 SEQUENCE_TARGET=155,24 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=14.RHAP61FE8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTAGGACTCTGAAGTATATTCAGGTACTTGTGATGCTGAGGGAGCCTGAAGGCAGCACCGAGTTTTGGTGTGCTGATAGAAACCAAAGATAGTTATTTGTCCCTTCTGCCTTTTAGGATCTGGGGAAACGGAGTTTCTTTTGGACCTGACAGCAGTTTGCATGNGNGTCNGNGATGTATTTTGATCACGTTCACCGTTATCTTGTCCCCCTCCCACCCCTACAGACCTTCTACCTCTGTGTGTGCATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCANTGGTGTTACAGGAAAATGAGAGAGGTTCGTTACACTGGGGCACCTTGCCAGTGGCGTACATCACTGACAAAGTTTCTNTCCCTCCCAGCAAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,401 SEQUENCE_TARGET=268,50 P3_COMMENT=CL is ACCGTTATCTTGTCCCCCTC P3_COMMENT=CR is CCCCAGTGTAACGAACCTCT = SEQUENCE_ID=16.RHAP61FF1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNTGTCATTTTCTTTTTAATCTCTGTTTTTCTGCCATTCTAGGTCAGCTGAAGATGAAGGTCTCCTCTACTGCTCTGGAGCCTNTNCACGGAGACCCTAATTTCATGCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTTCCNTTGAAATAATGGAAGATATTAAAGTATAAAAATAAGAGAGTTGTACCATCCATACCCATTCTGAAAATGAAGCCAAATGTAATCTTATCACTAAAGAGCATGTTATATNTGAACAGAGACATTTTATACACTATAAGATTAGATCTCAAANTNNTTATNTACCTGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,327 SEQUENCE_TARGET=120,44 P3_COMMENT=CL is TCCTCTACTGCTCTGGAGCC P3_COMMENT=CR is GGCTTCATTTTCAGAATGGG = SEQUENCE_ID=18.RHAP61FF3.seq SEQUENCE_TEMPLATE=GTAGCCGACACCATCCCCCGACACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACCAAACAAACAAACAAACAAATCAGCAAAACGTTTAGTCAATTCTGCTTTTCTTACTGGAAAGCCCAAAGCCTGTGTCTTAGCATGGCTGATTATCCTCTCTTCTGGGCAAGATTTCCTCAGTGCCAGTTTTATGTTTGCAAGGTCCCATCTATGCCAAGCATCATGTTAGCCCTAATATTTCTGATGCATTCCAAGCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,271 SEQUENCE_TARGET=34,34 P3_COMMENT=CL is TCCCCCGACACATACATACA P3_COMMENT=CR is TAAGACACAGGCTTTGGGCT = SEQUENCE_ID=19.RHAP61FG1.seq SEQUENCE_TEMPLATE=NNACNNACTTANTCCNNAAAGNANNACNNNNNNNNNNNNNNNNTGNCGNNNNNNNNNNNNNNNNNNNACCCATNNAGNCATACATACNGTCACATGTGTGTATANACANCNGNAGTCAACCNNGCNTCTTNTTCCNCAATTANANCNCCTNAANANNANNGNNNNNTGATAGGNCNATTACNTCNCCACTNACCTGNGTNNNNGCGGNCTCTNNANTTCGGGGNATCATTNNCTTGAACGGCNCNCCNNTGNTNAANNNTTTGGCNGNNGGNNGNTTNNCACNNANGATGNGTNNNNNGGNNNGCATNNNAGNTGTNAAGGCNTCGGGNGGGNNNGTATGGGAGGTGNGGNNNGTCNANNGGANGGNNTCGNNGGNGNNCANGTTCCNGNNGNNNGNNNNCNNGAANTNTTNNNGNNNNGGNNGGNGNGANGNNAGGATGNNAGNTCNNNNNGANNGTGNGNTTNGNNGNNTANGNGNNNCTGGGGGGGGNANAANNTTNNNGGGTGGTGTCGNTGGNNTGGNTGGTTTGNNTGCNTAGNNT SEQUENCE_INCLUDED_REGION=0,542 SEQUENCE_TARGET=29,14 SEQUENCE_TARGET=49,18 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=20.RHAP61FG2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNTAACAACTGAGACAGAAGAAAATGTCAGAAATGAAAAAAATAAAAAAAGCAGAAAACTNACTNATCAACCGTAAAAACAAACCAACCAAAATGTAAATTAAAAATCTAAAACACTTATACGTGACATTCAGAAATGTCTGTCAATGACTGAGAATTATTCACATCTAAACAATGAAAGAACACACAGTGCAATTATATCTGTATTAAATGTTTCCAGTTTGGGAGATATGCATTGAAGTACCAATATTGATCAAGATCTTTCTTGCGGGTGTGTGAGTATTAAATATGAATGTGNNAACAGTATGTTAACATCTGAGAGCATNCTCCATCATTCTGTCACCTCAGGTTTCATAGAAGAAATNTCCTATTGNCATGTTGTAGACTNTTTGGGAAAGNGNTANNNGCACTTTNAATCCCAGNNTCACCCTCTCAGGTCNTGGGATCTGNNCCACNGCCGTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGAAGT SEQUENCE_INCLUDED_REGION=12,509 SEQUENCE_TARGET=475,37 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=21.RHAP61FG9.seq SEQUENCE_TEMPLATE=AATGANTTAGAGGACCCCTTCCTCACAGGTCCAGAAGGACACTGAAGAANAGGTTAGCCAGATATGCTAAGGCTCTTGTCACTAGTCAGTTCTGAATTGCTTCCCTTTAATGTCTTGTCCGGGAAGGGACTGAGAATCCANTCANACATGTCTACCNTGNGNCTCNGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTNTCTCTCTCCTCAACCCAACCCAAACCACATTTCCCCCCCACACNNTCCCTCATATCNCCTTGNTTGNNATTCCCCCCCCCCCCCNACTNANANNTACNCNTNNNTTNTTTNAANNANAAAACCNCTNNNAAAAAAAACCNNNNGTAGNNNNNANANCNAAAATTTNTAAANANGNNNNNNG SEQUENCE_INCLUDED_REGION=0,479 SEQUENCE_TARGET=169,44 SEQUENCE_TARGET=215,40 SEQUENCE_TARGET=262,30 P3_COMMENT=CL is TAATGTCTTGTCCGGGAAGG P3_COMMENT=CR is GGTTGGGTTGAGGAGAGAGA = SEQUENCE_ID=24.RHAP61FH3.seq SEQUENCE_TEMPLATE=TAGAAATGNGACTGGTGAGATTTNANATTGTCCAANCATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATTTCAAACATTTCTGTGTGTGTTTCTCCAGATCTTCCTCTTTCTCTTCCTATACACATGAGAAAAGTANAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGCGGCGCGACACACACANATGGACAAATTGGNAAGANAGAAAGGATTNANTNNTTTAGACAACAGGTGTNNGAGATCNGGATTTTTCTCNTANANNTGTNGNCTGTTGNNAGGTNNCCAGGTGANTAGGGATNGGTGNNGANTTANNGGGGGGAGGTGCGNGGTNTNNCAGNNNNTTNATGGNTGGNGNTNGGTGAANNANANNTTCTCAGGNNNANNGNNGGGGGACGNATGTGGTTGGNNNNNNANNNNNANNNNNNTNTTGCNGNNGGNNNNNNGGNGNNTGNNNNTTTGNNTGANGGNNGGGGNGNNCNNCAAT SEQUENCE_INCLUDED_REGION=0,529 SEQUENCE_TARGET=43,42 SEQUENCE_TARGET=162,44 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=26.RHAP61FA11.seq SEQUENCE_TEMPLATE=TNCCANCNTCCNATGCACCCACNCNGCCCCAGGGAANNACCGNNNACNCCCGNCTTCCNGNNCCCCATCNTCTGGCACGTNNACCCGNGNANATGCNNNNNNNNNNNNNNNNNCCCNNACNNACACACTNNAAATCNNTANAANANGNNTTNNATTCAGATAATACTATNNNCCNTGNTTNACACTTNCNNNNNCGNCATANTGCANATAACATGTTATGTAGGCATNTTGTCTGATGNAGNGGNACATGGCCCAGGCCCNAGTTNGGATCCTTAGNCACGNNTNANAGGNNNTGGGNATGNTNATTCNGCCCACAGGTNTTGGGNTCGTCANNANGGGGTACAGANNGNGANTTNGCNANNATGNNNATAAGGATGGNNNCNTGTGCGNNNTTGNTANNGNNANNNNTTTNCCAGGNNNANTANGGNGGGGGNNNNNTNNAGTGTNANT SEQUENCE_INCLUDED_REGION=0,450 SEQUENCE_TARGET=99,14 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=26.RHAP61FA5.seq SEQUENCE_TEMPLATE=CTGTGTGAATTTCTAAGTCTTACCTTCAGAAGCAGTACCAGGTNGACATGAAAATACTGTCACTCTCTCAAATCATGTAATATTTCTGTTTGACTTGACATATGCAGAGAGTCTCTGTTTCAGCATAAGACATGCTAGCAGGCTTAAAAATACTGTTTCTGGCTGCTAAATCCTGAATCTACAAATGTGGGATCAATGATGTGAAAAGAGAAACTAAATGATGTGTGGAAGTTTATCAACTCACAGTGACAAGAAGCAGACTGCAGCAAGGTACCACACATTCAGGAAGTGAATGACTCCTTAAGTNATTCTCCTATAGAAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCNTGNGATNTATNNGCCATGAATGTTTNTGNNTCTAGATACAAGAAAAGGGNTGGGTATTTNTGNGGCTCCNACAAGGGGCTTANTCACCAANTTTCTTTCNNGNNGAGGGGTTNCGGCNCGNATTT SEQUENCE_INCLUDED_REGION=0,508 SEQUENCE_TARGET=324,56 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=27.RHAP61FA12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTCTGATGGAGCCTGTAGTCTAGCAGTATGTTTTCCTGAGACAAAAAAAGACACAGAAAAANATTGGAAGATTTAGCAATATGGAAGGATGCTGAAACCACAGGTATTCTTGGAATTCTTTCACTTACCTTAGTATGCCCCTTGTGNGGATTGAATGNNNTCCTGNAATACAGGCTGTCCTAGATTACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAGGTTTTGGAGATGAAAAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,258 SEQUENCE_TARGET=213,42 P3_COMMENT=CL is AAGGATGCTGAAACCACAGG P3_COMMENT=CR is TCCTTTTCATCTCCAAAACCTT = SEQUENCE_ID=27.RHAP61FA6.seq SEQUENCE_TEMPLATE=GTAAAATGCGTANNAACCAGCACAANCAACACACTGTTTGAAGGCAGGAGAATTTTCTCTCATTCTGAGGAACATAAACTNTTTATTATGNACAGGCCTTTGGTAGAACCAGTATAATGTTTAATCAAATATTTGGGCATCTCATGCTCTGTGAGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCCCACAGACATCTAGAAGAATCTAGACAAGATAACAGAACATTNTCAACTAACTGAACCACCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,281 SEQUENCE_TARGET=158,56 P3_COMMENT=CL is CAGGCCTTTGGTAGAACCAG P3_COMMENT=CR is TTCTTCTAGATGTCTGTGGGATG = SEQUENCE_ID=29.RHAP61FB11.seq SEQUENCE_TEMPLATE=GGCNNTATAGANNATCCCNTCNGGNCATNTCAANCCCACATNCTCTTANAANAACTTTGNAAAATGATGNNTGANNAAAANTTACTGCTNNNGNNNNNTCNACTTTGGNGNGNCTCTGNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATANGTGTCNATTTANNATGCANANCTNNNTCGGAGGTTGTGNTGNNCNCTCNAAAACTCNTTNTNNNTNCNTCCAGGGGNNGGGNNCNNCCGNNTNNNTCTCGNNCNTTTNNANATANGNANGTAANTCTGGNATCNTCCNANTNNACNNNTNAGNGAANNATGGGTNNNNGNAAGNGCTTTTNTNNNANGAGGGGGGGNGNNNNNGANNTGGAATGGGNANNNNGGGNNNAAAANNNGTTTTCCNTTGGNAANTTNNNNTCNNNNNNGANTNNNANAANAANATTNGAGNGGGAANCNNNAANNNTAAAGCNNGGGGNNCTT SEQUENCE_INCLUDED_REGION=0,490 SEQUENCE_TARGET=123,40 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=30.RHAP61FB12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTGCCTGAAAAAAGAAAATCACACTTTTTGATCTCTAGCCACCTAAAATGTTTGCTAAGTTTCACTTCCCAACACCAGGGGGTGTCTCTTCGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTGTCCTTAAGGTTCCATTAGTCCCAAACCTTTCTCCGTGTTTTTATGTACACTCTAAATCTTTCTCTGTCCCTCCAGAGGCAGGGATCAGCCTTATTTTCCTCGCTCTTTTTCATATAAGCACATAAATCTTGTATCCCCCCACTCCACCNNCAGAGAACCATGGGTCTAGAAAGTGCTTTTTATTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,332 SEQUENCE_TARGET=112,46 P3_COMMENT=CL is AACACCAGGGGGTGTCTCTT P3_COMMENT=CR is AATAAGGCTGATCCCTGCCT = SEQUENCE_ID=31.RHAP61FC4.seq SEQUENCE_TEMPLATE=CCTNCCCCNCNNCNNTGGNCCCNCTCAANNNNNNNNNNNNNNNNNNTCTNCANNNAGNNCGNNACCNTCNATCNCNAGNCANGNTCCNTCCCNNNAACNANNNTCNTCTATAGAAAACNCNCTGGNCGCNCTACGATNNGNNCNNACNNTNNNANNNTNGNNNTNNAANGGNCNNNNNNNGCNNCGNNGNNCNNACNNNCNNNTNNANNTNNCTNNNTNNNNTCGNNNNGNNCNNTNGNNNNGNNNCNNGCTNCNTGGCTTNNNANNNNNCCGACNNNNNNACCNTTTNCNNTNTTTNNGCNTGCNNCNCGNNCCTCNGGNNNNNNNGNNNNNNNNNTNNNGNTTNCNNNCGNGNNGGCNNNNTNNNNNGNNNNGNNNGNNNNGNTCGGNNNCCGNTNNGNNNNCCCNNCCACNNNCCGNTCGNNNNNCNCNNNCNNGCNNCNACCNCNCGGNNNNCNNNGNNNCNNNCNNCNCNGNNTGGNGCGNNCGGCNNNCNGNNGNTGNNNNNNNCNNCCNGNNNNNNNGNTCNNNNGTNGGGNNTCCNNNCCNNTNCNNNNNNNNNCNNNCNGNNNCGNNNGGC SEQUENCE_INCLUDED_REGION=0,580 SEQUENCE_TARGET=29,17 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=33.RHAP61FC12.seq SEQUENCE_TEMPLATE=TCCACATNNCAAGTTCCAGGTCAACCAGGGTTNNGTGGTGAGACCTCCCCCTTCTCTTACGCACACGCCAAACCTTAACAAACCCCCTAAAAGCCAAACCNTGAGAAAACAGAAACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANNTCANAGTCNCTTCNAGANTTGCATACCTAATATGNGCCNTTATCTATGTNNGCAAATGAANGTGAGTCTATANTTTNTNATACCANNGGGGGAGCNTTTGGGCNGCCCAGGGAGCTTTGGTGGGAGAGNATANAGNGGGGNGNNCNNNTCNTGGNAANNAGGAAATCTCNTTNNGNNNNTTNNTGNTANGTCCNGGAGTNTGTGTATNNNATCCTTNNGNTTTNTNNTNNGANATCNNTCCNCTAGGGNTCTTGGTCNNGGGTCNNNGTNNNNNNCGGGTGGAAAAT SEQUENCE_INCLUDED_REGION=0,469 SEQUENCE_TARGET=118,60 P3_COMMENT=CL is GACCTCCCCCTTCTCTTACG P3_COMMENT=CR is CTCCCACCAAAGCTCCCT = SEQUENCE_ID=34.RHAP61FD10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCTGGAGGATTTCTTTTTCTCTGATAGTATTTAATGTTTCTTTTTCTCTTCTTCAATTGAGGTTCCCTTTTCCTGTGTAAGTCAGAGTTCTTATTTCCACTTCAGTCTCTCAGACAGTTTCGATTACANNNNNNNNNNNNNNNNCCACTTAGATTTCTATTCAGGACAGAAGTCAAATTACTTTACTCGGCTGTTAATGCTTTTCAATTGCACAGAAAAAGGCTCTAGATCTTTGGTACCGAGTTTGCTAACCAATGTGACAGACCCTTTGTTTTAAAATTATGAACGGGGGAAAACAAATAGAGGAAATAAATTCNNAATAGCATGAATTTATACTTCCNGAAATGACCAAAGGGAAAGCCAGGTCCCATNATTACCTTGNGATGCAGAGCAGCNGGNTCAGNACAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,411 SEQUENCE_TARGET=146,16 P3_COMMENT=CL is TGAGGTTCCCTTTTCCTGTG P3_COMMENT=CR is TGAAAAGCATTAACAGCCGA = SEQUENCE_ID=36.RHAP61FD12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCTCAGCCCGTGTCATTTGACGCTTTCTTAGCACAATATTCCAGACTCTTTCACCTTTTGGTTATGATATAGTTCAAGCAGTTAAAAATCACGGAGGTTCATCACAGAAAGGGTGATGCTTGCTTTTTTTGGTACCAAGTTCTANCNTTGTTTAATTTCTTTGACCTTCCTCTACAATCTCATTTATTTTCAGTCAGGCGAGTGTCTGTGAGTGNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGCACTCATGTGCATACACACAGACGTTAGAAAAAGCCAGTTCCCCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,315 SEQUENCE_TARGET=236,48 P3_COMMENT=CL is TTCAGTCAGGCGAGTGTCTG P3_COMMENT=CR is TCTAGGGGAACTGGCTTTTTC = SEQUENCE_ID=36.RHAP61FD6.seq SEQUENCE_TEMPLATE=GTCTGAGACCCAGCAANATGNGGGACAGAAATAGAAGACCGACCTGTAATTTCATGGACAAGTTAATCTGGAGTTTACAGTACAGCGGAAACAAAAGANNTCCCGCCTCAGCAAAGTGGAAAGGNGAGAACCAANTCCTGAAAATTATCCTCTGATCTCCATTAGTGTTCCATAGCACCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNACATTNNNNNNNNNNNNNNTGCNANTNTCNCTTNCTNNAAAGANCAACNATGGNNGCACTTTCTCTCTTTAGCNGACCCANNGGNGCGGGTNNTGTNAACTCNNGNTTCTGNGGANNGGGGGTANCGGNGCNCGNNTTNGTTGNNCNTGGTCNTNGCNGTTTCCTTGTTGTGANNTTGTGNNTGCAGCGNNCNGTTCNANNNANNNTGGCGNGCCGGGAGNNTTNAGNGGAAANCCTGNNNGTTGCTTTNTGNNGGNGAGCTANGTCNCNTNNTTTGGGTNNNNNNNNNGGGNCNCTTTT SEQUENCE_INCLUDED_REGION=0,530 SEQUENCE_TARGET=182,46 SEQUENCE_TARGET=235,14 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=38.RHAP61FE5.seq SEQUENCE_TEMPLATE=NANACCNCCCCNANCCTGNCCTCTATCTCNNNCCNNNNNNNNNNNNNNNNNNNNTNCNTCCTCTCTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGAATTAATTNTAGGTGTGGTAAGCATTTGGTGCCAGCATTAATTTCCAGTCCCACCTTAAGAGNTTACTAGTGCGAANCTGAANTAANATTGACACAGTGAACGACGANTGCTGGGTAAGNGTCNNAGGGGNNCTTTGTNNTCACTCNCTACACTTAGGTGTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,320 SEQUENCE_TARGET=34,20 SEQUENCE_TARGET=69,82 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=39.RHAP61FE6.seq SEQUENCE_TEMPLATE=NANNCNCCTAGAGGACCCTGTCCNGCCAAACATTGTNTTCATGTCATGGACAAGGACAGGGACAGGGACCNTNAAGTTNCTTTTNNCCTACAGGCNCTGCCAATCCTCTGCTTCTGTCATCCCCATAGCATGTCNTAGNGCTGGACATTACACACCNTTCANCGTNNNCNNCNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNGTNTNNCACAAGCGCCNGCTATCNTGANCCCNNCATAATNNTGGCCGNGTAGTANTCCNNANTGNTTATCTGCTNNCTATGTTTGTANGNNNGANAGNAGGATTNATGNNNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,370 SEQUENCE_TARGET=177,50 SEQUENCE_TARGET=230,25 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=41.RHAP61FF11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNTGTCTGTCAAAACTCCGGTCATCTTCGAGACTTGACACTGGTATTTTTGTGTATGGACCNTTTCCTAGTCACATAGACCTCATTCACAGTGGATANTANTGNGTCTCCCATCAAGTCCCTGAGTCACTGACCTAAGTACAGGCTGTATCATTGACACCATATTTNNTTCTGTTTCTTNNTCGTGGAAGTGNTACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGATGTGNGTGNGNTGTANNGNNNNNATNTNGTANNNNNTNNNNNAGANNNNAAAGGTCAANNNNNAGNNCNNTNTNTGNNAAAAANNNANNNNCNTTANTNTGANANAACTNTCNCNNAGAANGGGNNNCNNNNCNCNAATTCGCTNNNNANGNNAANAGNNNGNNNCNNNNNGNGAAANNNNNNNNCGCNCAAAAAANTNNANGAAAAANANG SEQUENCE_INCLUDED_REGION=20,464 SEQUENCE_TARGET=216,50 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=41.RHAP61FF5.seq SEQUENCE_TEMPLATE=TNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNTGNNATCCCNGGNGGTGACTGTGGGNGCATCNGTNANNGNGGNNGNGTNNNTGTNNCNAANNGTTGTGGGAGTCNNTNNTGTGNCCCTGGTGGNTGNAACTANNTGTAGNTGNTAAGAACACCNGTGGNGAGNTAAGAGNTCTNNCATGGACCGGGNANTNAGNTGGTAANCGNNCGCNAACNCGNNATCANNNNNANANNNNGTTTCCNNGNNNNAAAANNNNNANCCNNNGANNNTNNCNNNANANANTNGNNGNCCGGGGAANANNANGNNNNNANCNTGGGGGGTGNCNNTANGCCGNGAGNNNNNNNNNNANNNNNNTGGGCNNGNNNTCNNNTGNNNNGGNNGTCNANNNNNNNANANGNNTGNNGGNNCNAGNNGNNTNNNGNGAAAGGNNGNNAANNTGCGNGGANANGGNNNTTTGNNTANNGGGAGNATGNT SEQUENCE_INCLUDED_REGION=0,494 SEQUENCE_TARGET=9,26 SEQUENCE_TARGET=37,14 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=42.RHAP61FF12.seq SEQUENCE_TEMPLATE=NNNNNNTGTAATCCTGTAATCCAGGGACGTGCTTTTCTGTGTTAGTTGGAAATCTCCAGTAAGTAAAACCATGTGNGNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTATAACTCCATCCATCCAATATCTAGTATACATNTATATACATTAGAGTTATTTATTACAAAGTACTGATGGACATGATTATNGANGCCAAGAAGTTCCAATTATTTTGAGTGAATTTCNNNAAAAGCCCANACTTANAANAGGAAAATAAAGCCTNTCTCCCTGTNGACAAANTGTCCNTGCGATAANCCTATAANCCCCNAACCCTTTTGGGAACAGTGCCAACCGNNNGGGGGNGGNAAAATTTCATATNNNTGNGGGCCCNNTGGGGNGTCANNTCTGTTNNAAGNNNCAATNNNNAAGGNNANNTTGNNANGGGGTGANCGNGGGNNTGGNNTTCGGNNTTNAANGGGGCNNNAGNTGTNTCCNGGT SEQUENCE_INCLUDED_REGION=6,483 SEQUENCE_TARGET=82,32 P3_COMMENT=CL is CTGTAATCCAGGGACGTGCT P3_COMMENT=CR is ACTAGATATTGGATGGATGGAGTT = SEQUENCE_ID=44.RHAP61FG5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNTATCTCTGACTTTGTTGTTTTGNTTTTCCGCANTTTTCATTGTGAATTCNATTTTTGNTTGNCTTTNATTTGCTGACNATCTCTTTATTAAANTTNANGTCTTGAATTGNTTCANTTATTTCACCCAACNANTCTNNNGGGGTANTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGCNTATGGGGGTGNNTTTTACAGTCTCCCNNNAGANATNNATGGTCTATNNTCNNTAAGANCNNTGGACATANTCATAANNACNANTCAGTTAACANNGCNCCNNNAAATTAGNNNNTCNATNNANAATTGNNAGTGGNNAGTCCNTGGCCNGGGCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=10,395 SEQUENCE_TARGET=159,84 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=47.RHAP61FH11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTGTCAAAATTAAAAACCAGCAAGAAAGGTCACTTNNATTTTAAGTGGAGAGAGGAAATAATTNANGGATAAAATATAAAACAATAATGAAAAATTAATTTCATCATGGACTGCNTATTAAGANNNANNTGTACATAAGNGNTATTGTTGTGTCANNAGTTAACTTCCCNGNTANCCCGNACAGGNNNTNGGGTCTANNANNCNNNANGGCCNACNTNAANNTTAAATGNCANNTTGTTTTCNGGNGTGGNTTCAATGCNCTNTGNNNTNNNNCNCNNNGNNNNNNNNNNNNNNNNNNNNTGNTGNNNNNNNNNNNNNNNNNGNNNANAANNGGTNTNAANNANTTANGNTNNNNNGNNATNNGNGNNGNTNGTGTGNNNNNNGGTNNTGNTNGNGTGNTNCNNTGTTGNTNNNNNNNNNNTNNNNGANNGNATNNTGNCNAANNNNNNCGNNNNNNNNNNCTTNTTGNANNGNTNNGNATTGNNNNTTT SEQUENCE_INCLUDED_REGION=19,489 SEQUENCE_TARGET=300,18 SEQUENCE_TARGET=323,16 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=48.RHAP61FH6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNTTGTGTCANACTNACAAAAATCNAACCAGGACACAATGTTNANCTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAGTTTATGTCCTTTTCATCTCTTGTAGCATTTAGTATGCACTGAGGCCATATCTTTTGAAGTGAGAGTTATACTGACATATTAGTATAAATTTCTTTTTGCATCTCTGNAGTTTTATAGGATTGTGTGTANTGTAGAATCTAACAAACTTTANTAAGTCTAGCATCTCACCTAAAGGAAAGNNCTCTGGGGGCCTACACTGGGGTGTGACCAAACTGCCTGGCACCAGTAGGCGCTCTCCATGGGGGGNCATTCTTCAGGTACAATAAGGGGTTGTCTGTTCAATACAGCNGTGGGGGGAACNTGCTGGCTGATAAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=10,433 SEQUENCE_TARGET=58,62 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=01.RHAP64FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNCAACGGGNTGGCTCAGTGGNTAGAGGTACTTGCCCACCNAGTTTTAACAACTGGAGTTCGGATCCCAGGGTCCCACGTCATGGAAGACAGGGAAAGNNTCCCACANTGGTTTACACACACTNCNTCGCACACAGTCACGCGCANGCGNAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGAGCACCAGAGGACGACAGAGGACAACAGAGAGCTNNNCNGTCACAGNCTCCNGACNNNNNNGTAATTAAGANNTTTGAGNAGNNNCNNNNGTGTTNGNTGNCAAAGAANAGNGNGGNNNTTNNNNNGNNAGTGGTGNANAGGNANNTTGGNCNGGNANGGAGGGCNNNNCCNTGGNGGNNGGNTNNNAAGNNNNNTGCNGNNNGANNGGNGNNNCGTGGNTGGGNNNGNANACNAGNNNNNNNANNNNNNTTGNNNNNGNNNTNNNNANCCNGGNGTNNNGAGNANNNGTTNNNNNNGAGNGNNNGTNNNC SEQUENCE_INCLUDED_REGION=25,495 SEQUENCE_TARGET=176,28 P3_COMMENT=CL is ACGTCATGGAAGACAGGGAA P3_COMMENT=CR is CTCTGTCGTCCTCTGGTGCT = SEQUENCE_ID=01.RHAP64FA7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCACANAAAACATCCTGTCNNNNNNNNNNNNNNNNTAGCTNNNNNNNNNNNNNNNNTATAAGTGCATGGAATGAGTGTGTATANNNNNNNNNNNNNNNNNNNNNNNNTTAGCTTANNNNNNNNNNNNNNNNNNNNNNNNTATAAGTGCATGGAAGGAGTGTGTGTATGTGACTGTGTGCTGAATATGTAAGCGTGNGGCGGTAAANNNNNNNNNNNNNNNNNNNNNNTAANTGNNNGACNNAAGCCNCAANGNGCGCTNTGTGCACTGTGTCNATNCGCCCGNGTCANCACNGCCACNCANNCANAACANGCCNNNAGCGNCGTGCATGCTGANCCGNCCCATATCNGNGNNNACCTANNNNACAACNNCANNNNCCGAAAGCNNNCGCCATCCCCNTANACANGNCCCCNNCNGCCACTNCNNCANCNNNGANNCACNTCTGANCCNNGCNCATNTCNNANNNANGACCNCTNNNTCCCGNC SEQUENCE_INCLUDED_REGION=22,483 SEQUENCE_TARGET=43,14 SEQUENCE_TARGET=62,16 SEQUENCE_TARGET=105,24 SEQUENCE_TARGET=137,24 SEQUENCE_TARGET=227,22 P3_COMMENT=CL is AAGTGCATGGAATGAGTGTGT P3_COMMENT=CR is CGCTTACATATTCAGCACACAG = SEQUENCE_ID=02.RHAP64FA8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCANTGACANAGCCTCTCTTGAAAATGAGGTTGAGGACCTGCCTCAGGAACAGGAGCCAGGGTCANTTGTAGCCTCTACACTCTTTTGCANTGAGGTGCAGGTCATATAGATGCAAGGGGCAAAAGGGGATCGGCACGTGCGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGACAAANACCCAGATTCATANAAAANACAGTNANGTAGGAACAGGNNCACACATACTTANACANNNGTAAACGTGGAAAAATGGCANATNNNNNNNNNNNNNNNNNNNNNNNNNNCCAGGGNNNNACACACCACACAAAAGANGTGGTNNNNACATNCNAACATNNACNNNAACNANNGTGNNAANTCACNNANTNNTNCNTNTNNTGGNAANNANNCANGNAAAAGTGTNNACACACTTANANNNAGGCAAATNTGTNNGNA SEQUENCE_INCLUDED_REGION=12,459 SEQUENCE_TARGET=156,50 SEQUENCE_TARGET=297,26 P3_COMMENT=CL is TAGATGCAAGGGGCAAAAG P3_COMMENT=CR is TGCCATTTTTCCACGTTTAC = SEQUENCE_ID=03.RHAP64FA3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCCTGGAACTGACAAAGTAACCCCAATGTTTGTTGGTAACGTCTTTAGTTCCATGAAACTATCACAGGCTTCAGAGTGCTGTTCTGCCCTGTTCACACTACAAATTCAATTTGATTCTCTTCCGTCTCTAAAGTTCTAATGTAAACTGTACAGCTGACAAAGCATAAATAAGGCTTTAAATGCTAAGGGTTCGCTGTAACAGGATGTAACTGTAATTTACTCTCAAATATATCTTTTAAATCTCCACGTGTGTCAGAAAGAGACAAAGACATGCATGCTTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAAGAGGGAGAGGGNNAGGGNGNAGGAAATTGNNAAAATGAAA SEQUENCE_INCLUDED_REGION=21,423 SEQUENCE_TARGET=304,34 SEQUENCE_TARGET=346,54 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=04.RHAP64FB7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCTCCACTGCAATCAAAAGATGANCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTCTCTTATAAGACTTAGGATATTAAAACAAATGAGAGAAATGGACTCTAGGGTATCCAATAGTAAGTCAACAAGGGTTGGTGAGTTCCTGTCCTGTAACAGACCCAAGAGCCACCCACGTCTAATCTGNAGGGANACAAACAGGGGGGCAATAAGGGAATGGGAGAGAGGAAGANAGTCTCATGTCACGCAGAAACGNTCTCCCCGAGCATCNCAAGTGGAGACCTGGGAACAGATTCAGGTAGATTGTAGGAATACAGGGGANATGCCCTAGGNAGCAGCAGGTAGCTGGAGAGAATCNAAGGCTTCTGGGAGCTGATGTGAGAGCATGCACNGAGAAGNNTCCTGCATCTGTCTGTGTCTNAGTTAGTGGTTTCACNNC SEQUENCE_INCLUDED_REGION=12,455 SEQUENCE_TARGET=39,44 P3_COMMENT=CL is CCTCCACTGCAATCAAAAGA P3_COMMENT=CR is CAGGAACTCACCAACCCTTG = SEQUENCE_ID=06.RHAP64FB3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCCTTTAAATGATCAAGGAGTATCAAAANTTCCTCATTTGTGAATANANCTACGTGTATGNGTGTGNNAANGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTATCCGTACATGGATCAGAGAAATATAATCCTAAAAATCACAAAAATGACATAAAATGACATCTGTTATTTAAAAAAATCACCTCAGTTCAATGAGAACTAAAATATTTAAATCAACTACAGGAAAAAAAAACACCCCAGCGATTACACACACTGATTCAAAGATTGTGGCACCATTGACATATATTTCATTTTTGNTATAAAGTTTAANGTTTTGTAGGGNTGACTTGAACCCTGAAGAAGGTACCCAGGGTGCAGGTCAGTGCTGCATATGTTATTTACCAGTGCTAGACACTCAAACTTCAGGGTNNNCAC SEQUENCE_INCLUDED_REGION=21,421 SEQUENCE_TARGET=97,28 P3_COMMENT=CL is CCTTTAAATGATCAAGGAGTATCAA P3_COMMENT=CR is TTTCTCTGATCCATGTACGGA = SEQUENCE_ID=07.RHAP64FC7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCAGCCAAANATCGTCAACAAACGCCTGTGACNACCCCCCAAGATCAAAATCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGTGTCAGTGTCTCTCCCNGCACCCATGGACATATGCATGTGCCGACCCAATANGCGTCCATNTATGCATATCCATGTGCATCTGNNCATGTATGTGCACATGTGTGTCTCTCAGTGTGGGTGNACAGGNGTANGGGACATGTGTGCACATGGGCGCANTCCAAATAATCNGGAACTCTTCCCCAGCTTCACCTTGAAGGCATTCCCAGGCGCAAGCAANAGCTANCGTAACCACNGGCAGCATTCTCTNCTCNCCTCCGACCCCTNTAGCTCTCGCCNCATCAGGCCGTAGGCTGNCCCAGCTTCAAACTGNTAAGAGCNTGTCACCCTCCANNCAGAGGNACCCGTGNGNGTGNGGGTCNGC SEQUENCE_INCLUDED_REGION=12,448 SEQUENCE_TARGET=67,29 P3_COMMENT=CL is CCCCCAAGATCAAAATCCAC P3_COMMENT=CR is ACCCACACTGAGAGACACACA = SEQUENCE_ID=09.RHAP64FC3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTGACATTCTCTTCCGACCTCTGCAGCCATCAGCATATGTGGTGCACAGACATATGTGTAGGCAAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACAATTGTTTTTAAGGAAAAAAGTAAAAACAAACAGGAAGAAGAAATGTCTGGTCTACCTTAGGGCCCAGGTTTTAGTGTAACAAAGGCTTGTCTCCTCCCAGGACTTACCATGTTCCCCTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,239 SEQUENCE_TARGET=89,45 P3_COMMENT=CL is CTGCAGCCATCAGCATATGT P3_COMMENT=CR is AACCTGGGCCCTAAGGTAGA = SEQUENCE_ID=11.RHAP64FD8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTTAATTAATGCTTACTGACGGAAAAAAGGACAGGGACAATAATCACCTACTGAACAAAGAAAGTGATATGTATGGAAAANTAAAAGCAGATTATTTCACATAATGTAAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATGCTTGCAGCGGGTGCCTATCTTAATCCCAGCAGGCAGACTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,193 SEQUENCE_TARGET=135,30 P3_COMMENT=CL is CTGACGGAAAAAAGGACAGG P3_COMMENT=CR is GTCTGCCTGCTGGGATTAAG = SEQUENCE_ID=12.RHAP64FD9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNCCACTATATCCCCTGCCTTTGTGGACCTGAGCATCTCATTGACGAATCCCAAATTATACCCCCTTCATTATTCATTAAAGTGTGGTGGGCAGAAATGTGTGTTCACGAATTCAGCTATGGAGAGGTTATAACTGAAGCCTCTGAATGCCCCACAGGGACCAATGTCAGCCAGTTCTATGCCCCTGTTCAGTATCTGCTTGAATCTGAAGCCGGACTCTGTATGTATGTGTATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAAGANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCANATATATATANNTTNTCNNNGGGGGGGGGGGGGNGAGGGAGAANANNNNNNCGGAGGNNNTANNNTGNGCNAGGTGNNNNNNTATNNGNNNACNCNCNTCNNGGGGC SEQUENCE_INCLUDED_REGION=13,445 SEQUENCE_TARGET=246,44 SEQUENCE_TARGET=302,46 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=14.RHAP64FE2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT SEQUENCE_INCLUDED_REGION=19,432 SEQUENCE_TARGET=44,46 SEQUENCE_TARGET=92,33 SEQUENCE_TARGET=140,26 SEQUENCE_TARGET=170,14 SEQUENCE_TARGET=211,20 SEQUENCE_TARGET=238,26 SEQUENCE_TARGET=336,14 P3_COMMENT=CL is CACATGTGCACAGTGGTATGT P3_COMMENT=CR is GCCTGTGATGTATGTATGTGAGA = SEQUENCE_ID=15.RHAP64FE3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCGACGACCTGACTTGGATCTTCTGGAACCACCTGGTAGAAGGGAGAATATGGTTGTCTTCAGACCACTGCAAGCCGTAGTAAATGGATGTAAATATATGTGCACACACAGAGTAATTATAAGTAATGAAAGAAAAGAAGTAAACACTTTCCATAATGTAAAGAAGAAGGCAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTATTCTTCACACAGTGACCCTCTCCACAGAGTCTCTCCTGTGTATTCCGCACACAGTGATCCTCTCCACCACCACCAATGACTTTTCTATAGCACGAATCTAATTCCGCTGGTAAAAGTCCTGGGTTGGCATGCCACAATTTTTCTTGTTCTTTTTAAAAGTAATTTTAGAGTAAATTTACAAAGTAATTNGTATTT SEQUENCE_INCLUDED_REGION=20,420 SEQUENCE_TARGET=194,45 P3_COMMENT=CL is TCCATAATGTAAAGAAGAAGGCAA P3_COMMENT=CR is TGGAGAGGATCACTGTGTGC = SEQUENCE_ID=15.RHAP64FE9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNCCTGAGCACCAGAAACCACAGAGTTGTGCTATACNNNNTGAGCAATAGCCCAAGCTGCGTGTTAGCTCTGCACAGCATGGTGCTAACTGTGTTGTTTTGTCAATCCCTCACCACTANCTGTGNGTGTGCANNNNNNNNNNNNNNNNNNNNNNNGTGNNCANGNGGGTGAANGTGTATGNGTGNGCNTGCGNNNNNNNNNNNNNNNNNNGCATGNGNNNNNNNNNNNNNNNNNNNTATGNGNATGNGGCGNGAGNGTGTGNATGTGTGGTNTGCATGTATGTGNNNGTNCAATGTGGTANNNTNGTGNGGCANNCNTGNNNGTGTGNTGNTGGNNGTGNTAANNGGTGNAGNGTGNGNGCGGGCGNGNGNTGGTNTNGTGTGNGGTTGNGGNNNNGNGTGTNGNGGNNCNCNNGGANNGCGNNNNNNNNNCCGNGCNGGNTCNNGGNANTANTNGNNNANNGNNNGNANAGNNGAC SEQUENCE_INCLUDED_REGION=13,475 SEQUENCE_TARGET=143,23 SEQUENCE_TARGET=204,15 SEQUENCE_TARGET=229,18 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=16.RHAP64FF7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCATTCTTTGCTATTTATTGTTCCTGGGTAGCATTTCCTAAATACATCCATGAAGTGCTTTTTCTGTAGAAGGTTAACTTGTGTAACATGTGGCAGGACCTAGTACTGAGTGAGTTTGCCTTCACTTCAGTCTCACAAGAACTTGAATAGGCTGGTGTCTAATAATGGAGCTGTTGAAATGTTGGGNTCCTGAACTTTTCAGATTTGTCAATGGACTAGGGTCATGTGAAACACACATTGAAGAGAACTTTCAGTTGGACTNTTNAAGGGTANCCTTTTNNTTTTTAGAGATCTTNATNATTATTTTTTGCCACGNGTACNNNNNNNNNNNNNNNNNACTANGTGCATGTAAGTACAGACGCCTGTGGAAGGTGGAGANTTGGATCCCCTGGAGCTGGAGTTAAAGGCGGTTATAAGTTAGGAACTGAACTGAGGTCCT SEQUENCE_INCLUDED_REGION=23,438 SEQUENCE_TARGET=343,16 P3_COMMENT=CL is TTGTCAATGGACTAGGGTCATG P3_COMMENT=CR is ACCTTCCACAGGCGTCTGTA = SEQUENCE_ID=17.RHAP64FF8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCCCAGTCCAAGTTTGCAGTAGTTCCCTAGTTAGGGAATATTTTTCTGATGACATAGGTGTAGTCTCATCACTGTGTGCTTACTGCCTAGATTAGCACAGAGCAAAGAATGAATTGGTTTTCCAGGGAAAATTGCTAAATTAGTAAACACGAGAGTAAGGCAGGAAAGAGCAACAATTTTACTCACAGGTTAATGGTTCACGTTTAGTCAAATTCCAAACTATGGCTCGGTGGTTGGTATAATTATAAGGACATGTTCTATGATTCCATCTCTTTATTAAATATCTCTAATCNAGGGACTGCNAGGAAATGGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGCACTCACGGTGTGCATGTGGANNNNNNNNNNNNNNNNNNNNNNGTGTCTGAAATATNTCTTGTGTGTGCNAGGNG SEQUENCE_INCLUDED_REGION=23,443 SEQUENCE_TARGET=340,48 SEQUENCE_TARGET=412,22 P3_COMMENT=CL is AAACTATGGCTCGGTGGTTG P3_COMMENT=CR is CACATGCACACCGTGAGTG = SEQUENCE_ID=18.RHAP64FF9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNCCTTGGAAAATCACTGGGAAAGGGCTAAGTTGGCACGCCCCTTAGAGCAGGGAGGAACTNGGTGGTCGGCAGGGTCATTATGACATCTTGTGGTTGTTCTCAAGATTTACCACAGGGAGGGGTAGGGGGGTCTTTCCGAGAACAGTATCTTTGTTTTGTACTAACTTATGGTCAGAATAGTCTGGTCATTCTGACTACTANAACTTTGTCATAAATTAGGCAACCGGAAGTCAGATGTATTTCTCAAAACCTTGTTTTTACAACTTTGTTTCTTTGNNTCNCATATCTATGGAACTATGAAACCTTANTTCNTCAGNGGGGGGGGGGGGGAGAAAGACNNNNNNNNNNNNNNNNNNGAGNGGAGAGAGGANAGNGGANAGACNGANNNAGNNANGANAGACANAGACAGAGNACNGGAAAGAGNNNNNCAAGNNNCAGAAGGNGNGNNNNG SEQUENCE_INCLUDED_REGION=13,451 SEQUENCE_TARGET=351,18 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=19.RHAP64FG1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCAGAGAGTGATCTACCTACTGTTGGTTCCAGAGCCTGAGTCTGTTACAGCGGTCTGCACACACCACCATTCCCATTTCCAAGATAAGGAAANTGGGTCAAGGTNTTTGGGTGAGCGTGCGACAGATGAACTCATGGTCAAGGACTTCTTGGCTTTGAAAACCTGACCCTCACTCCCAAGTGTTCTCTGGGCTGGTTTGCCTCAGTTGGTTTTAACAGACTGGTCTAAAATTTCACCTCAAAATATAGAGCATGAAAGTAGAAATAAAGCTTCAATATCTGTCCAAATCTCATTTGTTCTATTTTTTATCATCATCATCATCACCACCACCACCATCATCATCATTACCACCACCATCATCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACNATCACCANCACCATCATCACCACC SEQUENCE_INCLUDED_REGION=18,427 SEQUENCE_TARGET=381,35 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=21.RHAP64FG3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCCAGCCAATCTAACCAGTTAACATGCTCCAGGTTCAGTGAGAGACTTTCAAAAGGAATAAAGCCATAGAGAGGGGCATCCAGTGTTGACCTATGGCATGNNNNNNNNNNNNNNGGCATGTGCACCCATGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGCACTTCATTAGTCAAGTACAGCAGAGTGAACAGGCTAGAAGGTAACAGGTGACCACGTGTACCTCCCTCCACCCCATCCTCCTACCCCCACACACCCTCTGCAGGGAGCTGCCTGGAGAAACAGNAGAGCCTGTGGTAAGGTGTGCTGGGAGGGTGGAGAGTTAAGGAAAAGTGCAGAAAGCGATCTGTGTGGTTTCCACAGAAAGGNATGGCTGCCGTGGAATGTGTCCGGG SEQUENCE_INCLUDED_REGION=21,423 SEQUENCE_TARGET=120,14 SEQUENCE_TARGET=152,55 P3_COMMENT=CL is TCCAGTGTTGACCTATGGCA P3_COMMENT=CR is CACGTGGTCACCTGTTACCTT = SEQUENCE_ID=22.RHAP64FH7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCACCCCTTGTTGTCTTTACTATGNGCCTCTATATGACTATACGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNTNNNTCNCTCTCNTNNNNTNNANANANANNNNNNNNNNNNNNNNNNNNNTGNGNNGNNNGTGTGNGNGTNNNTCNNTCNNATNCNNNANNGCCANNTGGANTANNNNANNNTCNTNNNAANTAGANNNGGGNNNTNGNANTNNCCNGGAGGTNTCNCANCCACNNCTCNGCANANGNACANNTANNCNNCAGNACTCACATNCTNNGANANACNNNTNTGTTCTGGNNNCCANTNNNAANGNTNNNTTNNNNATAATNNNNNGNNCTCNNCANNGTGNNNNTCNNACTCCNNNNTANGNNTNNNGNTTNCAGNNGNANNNNNAGTANANGNNACNTCNNGNNTNNTGATGGNNNNNNNNNTNTNNGNNNCCNNNTNCNGANNTNNNNNNNNTNG SEQUENCE_INCLUDED_REGION=12,488 SEQUENCE_TARGET=57,22 SEQUENCE_TARGET=137,18 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=26.RHAP64FA11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCATCCTACTAAGGGAAAGCAAAAAAAAAACTTCCTCCTGACTNTGATGTTGAAAGAAGAATGAAACGAACATCTCTCCAACAATATCTAATCAGCAGGAGTCATACTCACCCTTAGGTCTAGATTTACACTTTTCCAAGATCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGGGGGGGTGGAGAGGAAGAAGGACAGAGACACAGAGAGATCACTAGACATATAATACACTACGAATTGTACAATTAAGAATTCAAAAAATTAAAAAAAAAACCATTTCTGATAGCCAGACAAGTCAGTTTGATGAACAAAGAGGAAGAATGTGAGTGAGGAAGGATGNAATTGGAGAGAGGGATTGAAAATGGCAGTANTGTTAAAGATTGGAGAAGAAGGAAGTCCTGTGGGAGAGGGGGTGGGGGAGA SEQUENCE_INCLUDED_REGION=12,445 SEQUENCE_TARGET=157,46 P3_COMMENT=CL is CAGCAGGAGTCATACTCACCC P3_COMMENT=CR is GTCCTTCTTCCTCTCCACCC = SEQUENCE_ID=30.RHAP64FB12.seq SEQUENCE_TEMPLATE=CCTTCCTATTTTCCTCTCCAGTGCTGAGACCAAAGTGAGATCAAAGTCATGCATNACCATGGGNACCTTCCTTTTCANNANTTTNTTTCATCNTAGGAAGTANGTANANANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTTAAAATCAGCACTCAAGANNTNTNNGNTTCCTATCCCTATANCANNCCCCCCAAAACANCCNNAAAGCCACCNGCNCCAGAGAAAGAGGGAGATGAAATCTCTAGTNNGAAAAANNAGNNGAGGGAGATNACAGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGNANANACACAGATACANNNCCNNANANNNNGGNGANACNNNNNNNNNGNNGCNNGCGNGCNNNGAGTANTNNANAAANANNGNTNCTNNNNGNGNNNCNNGGNGNNNA SEQUENCE_INCLUDED_REGION=0,451 SEQUENCE_TARGET=113,32 SEQUENCE_TARGET=146,26 SEQUENCE_TARGET=314,24 P3_COMMENT=CL is TCTCCAGTGCTGAGACCAAA P3_COMMENT=CR is TTTCATCTCCCTCTTTCTCTGG = SEQUENCE_ID=31.RHAP64FC10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNCCTAACCCTTTATGCTATCTAGTTACTTCCCAGACATGCACACACGCACGCACGCACGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGCCCCAAGTTACTTGCATTTAGTCTCACCTTGGATACATTCAGCTGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=24,167 SEQUENCE_TARGET=86,52 P3_COMMENT=CL is CTTCCCAGACATGCACACAC P3_COMMENT=CR is CCCACAGCTGAATGTATCCA = SEQUENCE_ID=31.RHAP64FC4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCTGTTGGAGCCCAGCAAAGATGTTTTCTAGAATTCCCAACTCAGACAATCCTTGGAGGTTGTTATGTGAATGTATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACACAGGCTCATGCCTTTGTGTGGAGGTCAGAGGCATCATCTAGCTTACATTTATTAAGACAGCGTTTCACATTTTGCTTGGAATTCATTGATTCAGCTGGACTGGCTGTTTGGAGAGCCTCAGGGATCCATCCACCTGCCTCTGTCCCCTTGACCCTGGGATTACAAGCAGTCATACCCAAACTCAGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAACTCAGAACTTCATCTTGTGTAGCAACTGTTTTTCTAAGCGAGATGTCT SEQUENCE_INCLUDED_REGION=20,416 SEQUENCE_TARGET=99,48 SEQUENCE_TARGET=340,44 P3_COMMENT=CL is AGCCCAGCAAAGATGTTTTC P3_COMMENT=CR is ATGATGCCTCTGACCTCCAC = SEQUENCE_ID=33.RHAP64FC6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCCAGAACAATGTCATCTGGACAGCTTGCAAAAATGTTAGCATCTGTTCTTGGTCTACCAATGCAAGTCTATGTGGTGAAGGTGTTTGTTATTTACAGTTTAGGAAATAAGGAATAAATCTTGAGATCATCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACTGGAAGTTATTGGTAAAGNGCACAGAGGCTTTCTTTTCTTTTCTTTGTTAANTTCTTTTCAAAAAGGATAAAAAAATATCTTGTCAAACTTAAAACAGCTTATGAATTCTCCCCACTTGCTTCCCAACGTACTGTTTTCTCTGTAATTATACTGAATAACAAAGATTCTATATTTGTCNCATAGTTCTNGCTACTTAC SEQUENCE_INCLUDED_REGION=17,428 SEQUENCE_TARGET=149,40 SEQUENCE_TARGET=190,54 P3_COMMENT=CL is AAGGAATAAATCTTGAGATCATCG P3_COMMENT=CR is AAAGAAAAGAAAGCCTCTGTGC = SEQUENCE_ID=34.RHAP64FD10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNCACTAGGGAGCCTTTATCAAATAAGTAGCTTGACCAAATGCCTTTTCAGTACNNNNNNNNNNNNNNNGCCTTCTCTTTTCTGTATCGGACTATACTTCCCTGAGAAATTCAGAAGAAATTGAAAGGTGAACTACTTCACCATCTCCCCTCTGCGTGCATCCATCTGTTACAAAGGATATCGTTTGACATATATAACCATGTTAACCTTCACGATTTTAAGGAAGTCCTTGGTGGAGGACCACAAAGAATAGATTGACCTCAAAAGGAGGTCTATTGGTCTGAAAAGTTGGCACCCCCAAGTCCTGTGGCTGGGTACCCAAGGATACAAGAAAAATCACCTCGAAGGGTCTGAAAATCAGTTTTGTAGTTAGGAGGAAGAGCNTGGTAAACTGCTCTTCAAAACTACCTGATAGGGGAANATGAGTCCATTGTCTGGTTTGTTT SEQUENCE_INCLUDED_REGION=24,443 SEQUENCE_TARGET=76,15 P3_COMMENT=CL is GCTTGACCAAATGCCTTTTC P3_COMMENT=CR is GTAACAGATGGATGCACGCA = SEQUENCE_ID=35.RHAP64FD11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCTCCTGAGAAATGCAGGTCAGGGTACCTTGGCAGGGAATCCCAAGGATCAGTACATGGCAGCTGAAAGTTGTCTTGGATGCAAGACTGGGAGTGAGGCTCAAGTCAGCCGAGCCTCAGCTCTGGATTCCTGAAACAGAATACCAGAATATGTTAGAGAGCGAGGAGACTAAACTAAACATCTGGGAGTCCTCAGCAGATTGTACAGATCCAGGGCTGAGGGNTCATAAGTATGTTGGTCTTTGTGGGGGTGGGAAAGGAACACTTATCTAGATCCCCACCTCCTTACTATGTGCTACTACGGGTGGNGGCAATGCCTTAAGGACCAAGGTTTTTTGTTTTGTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGTGTGTGNGTTGCATNNAATGCANGGNGCAGGTGGTANTAATTGNNGGGGGNTAANNCANTGTTCTTCNTCTACCNNNTTCC SEQUENCE_INCLUDED_REGION=23,460 SEQUENCE_TARGET=369,28 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=36.RHAP64FD6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCACATTTTTCTTTANGNNNNNNNANTGNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCGNNAAGGCACAANTAACCAAGGANGGAGGTCAGACAACAAGTTTATGGCGTCAATTCNCTCTCACCTTTACATGGATNGCCNGNNNTACACTGCAAACATCCGGCACTGNACCGTTCTGCCAGTCCANGGTCTCCCNANNTTTGTCCCCCTCNCTGTCTCNCNGTCTCTGCCCTTGNTAGTCTGNCGNNNCTCCGATANCCTCNNNACCCCNGGNNTTCTGCTCTCNGTGACTTCCCCCTCNCCCTGNCTCCCNCNGTCNGNTCCGTCTNGTCCNTGCNNTTNCCTAGTTTCNCNCCNNTNNGTNTCCCNGGNNTGGCGNGGTCNGNNNGTGCCNCCNNNTATNGTGA SEQUENCE_INCLUDED_REGION=27,434 SEQUENCE_TARGET=57,46 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=37.RHAP64FE4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCAGCCTTTGATACAGGAACAGGAGGACCCAGTCCGTAAAACCAAAGGTGAGGGAGAAACTAAGACATTCTCAGATAAAGAAACTAAGGAAATGTCTTGATAATGTAACAAGCCTTGGAAACAGCTTTGGCTACAAAGCACCGCTTCCTCTCATGGAACCCACAGCAATCGTTGGCAAGGGCAGTGAGACAGACGGCTCAGCAGTTCTAAGTACTCGGTGACCTGAATTTAACCCACATGGAAGACGGCAACAATCAACCCTTGAAAGTCTTCTGAGTGTGTACCCTGGCACCAAAGGACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTAGGTTTTTCTTTTTTCTTTTTCTTTTTTTAAAGTAGAAGCAGGCAGCCGGAAGTGGTANCTCACAATCTTTATCTTTT SEQUENCE_INCLUDED_REGION=20,428 SEQUENCE_TARGET=322,44 P3_COMMENT=CL is TCTGAGTGTGTACCCTGGCA P3_COMMENT=CR is CGGCTGCCTGCTTCTACTT = SEQUENCE_ID=38.RHAP64FE5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCATGTAGAATGTGTTTGTTTGTTTTTGTAAACTTAACTTAATCAAGGATATTCCTCACAGGCATACTCAGAAGATAATCTACAAAATCCCTCACATGATGTAGACANGTGGTCTACATCAGTAGGTCTCCACACTCTAGTGGCTGTTTTCTCCAGGTAGTGCTTAACTCTGGCTTTTAGCTGTTTCTAAATATTTCCTGGGAAAATTAAGGTTAACGAGAAACATATTGACCTTCGNTGTTCTTCCTGTGTGGATAATCTGACTTTGGACAGACCTAGGTTGTGAGGAATGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTGCGCGTGCCAGAGGTCGACCACAGCACAGGTGTTCTTTCTCAGAAGCAATCTACCNTATTTTATTTTATNC SEQUENCE_INCLUDED_REGION=17,424 SEQUENCE_TARGET=310,56 P3_COMMENT=CL is CCTAGGTTGTGAGGAATGCC P3_COMMENT=CR is TGCTTCTGAGAAAGAACACCTG = SEQUENCE_ID=39.RHAP64FE12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNACCCCCCANAGCGTCCGTCTCNGNNNNNNNNNNNNNNNNNNNNNNNATGNANNNNNNNNNNNNNNNNNNTANGCCNGNNNNNNNNNNNNNNNNNNNNNNNATATGTCNGTGTGCCNCNNNNNNNNNNNNNNNNNNTATATCNGTGTATATGTGNGTGTACCCNNATGTGTGNGTACNCCTGTCNGTGNATGTGTGTATGNCTCNGCACCNCNCNNNCNCNCTNNATGNCNNNATANNTANCTCNANGTGNNCGCCCCNATGNACATNNCNGTGCCNACANNTCNGNATNCGNCCCNCNATCTCNANCNANGTCNGNNNNNANGNNNGCATATCNNNGTANNCNNANNTCNNTCNATACCNATCTANNNGNNNNNANCNANNNNNCNNNNNATANCCNCNCNCNGTCNCCNATNTANACANNNANNNNTNNGT SEQUENCE_INCLUDED_REGION=23,432 SEQUENCE_TARGET=47,22 SEQUENCE_TARGET=74,18 SEQUENCE_TARGET=101,22 SEQUENCE_TARGET=140,18 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=39.RHAP64FE6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNCCTTTACTTGAATGAAAAGTATTGTTTCTGTCTGTCTCTGTCTCTTTGTCTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGNAGGGNGCGTTACCCACCATAGATTATCCCCGACAATTTCAGTCTTCTCTGAGAATATCATAGTANAGACCCACCAAAATTGTCTCATTTNCAACACTGATCTTGCAAAGAAAATTAACATAAATCCGNNAAACTAATACTGAATANTTATAATATATCCATATCATGTCAACTATTTACATTCATAGAGTCCTCATCTAGAAAAACAATANTCTTTGTTTNTACAGTGGTTTGTACATGGC SEQUENCE_INCLUDED_REGION=16,425 SEQUENCE_TARGET=70,36 SEQUENCE_TARGET=107,54 SEQUENCE_TARGET=162,32 P3_COMMENT=CL is TTCTGTCTGTCTCTGTCTCTTTGTC P3_COMMENT=CR is TCGGGGATAATCTATGGTGG = SEQUENCE_ID=41.RHAP64FF11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCATGATATGGGCGTGGTTTGTGTGCTTGCTAAGTCAGTGAAGCACTCTCTTGAAGGAAGGAGGACCTGAATACAATCCCCCAGAATCTATGATGAGTTGGAAGCAGAACTGTCCATAGTTTTGCAAGACTGTATTTCTTGTGCTGGTATGATGGAGTCACATGATCCATTCAATCATTTGCCAGCCAGACTAACCTCACTAGTTAAGTTTAAGGCTGATAAGGGACCAAATATTAAAAAGAAAGTTGATTTCCAGGAGGAACATCTGAGGTTGTCTTTTAGCCAGTACACATTTGTTTGCAAATATGCACACATACTAGAGAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGAATGGGGGGAGTTCAGAAAATCTCCAGGGATCNGTTNTNA SEQUENCE_INCLUDED_REGION=23,446 SEQUENCE_TARGET=348,74 P3_COMMENT=CL is TCCAGGAGGAACATCTGAGG P3_COMMENT=CR is TTCTGAACTCCCCCCATTC = SEQUENCE_ID=44.RHAP64FG5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCCTCTTTCCTCTGTAGAAAATGCACTGCTAATAACTTTGCTCAAATATATCTCACAACATTTCTTAATATGGCTCCAGGTAGCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNACAGACAGACAGACAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCAGAAAGAGTGAAACCTAGGTACCCTGGTATTATAAAGAACACATTTTCAGCTCAAAAGCGAGCAGGGNGTCGCTCCACTCTCCATGGGTCTGGGTGTGGCTCCNTCNCCACACTGAAGGGTCTGNTGAGCTCCTGNAGGGGTAACAGGGGCTGCNTCCTCANATTANGGGTTCAGAGGNTGGNGGGTGNGCCACATTTTTTCAAANACA SEQUENCE_INCLUDED_REGION=17,435 SEQUENCE_TARGET=103,40 SEQUENCE_TARGET=144,22 SEQUENCE_TARGET=186,54 P3_COMMENT=CL is TCCTCTGTAGAAAATGCACTGC P3_COMMENT=CR is CAGACCCATGGAGAGTGGAG = SEQUENCE_ID=45.RHAP64FG12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCTGTCAATATTCCAGAACAGCGCATGCCACCCACACAACGTGCTTCAGCCAGCCCTGCAGTGCCCTTCCTGGACAGGATTTCTTCTTCTTTGAAAGAAAAACAACAACAAAGCTTTTATTATANNTATCNAATTNNNNNNNNNNNNNNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGTATTTGGACATAAAGATGAGACTGGTAGGAAGCTTTGTGTTGGAGACTGTCCTTAAATTCCCTCCATCTTTCCTTCAGGGGAATAGCCCATGCCTGTGGAACCCATCGCTCCTCACTGGGTAGCTGTCAGAAGTGTGAGGATGCTAACAGTCTGTAAACGGTGCTGTCCCTGCAGCGATCCCCAGCAGAGTCTGAGAAACTCCCAATCCACCAGCAAGATGACACAATTGTTTTGCTG SEQUENCE_INCLUDED_REGION=22,442 SEQUENCE_TARGET=156,14 SEQUENCE_TARGET=172,50 P3_COMMENT=CL is TTGAAAGAAAAACAACAACAAAGC P3_COMMENT=CR is CCAACACAAAGCTTCCTACCA = SEQUENCE_ID=46.RHAP64FH10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCCAGCCCTGCTAACCACCTAAAGGCACCAGCAGGAGCTNCCCTCAAAAGATCTCACGTTTCCTCTGACAGCACCTTAAAGGAGCACCANTAATGAAGGCTGCTCTGTTGNGTGGGTAGGATGCCAAGGCAGAGGCGGCTTTCTACAGAGCTCCTTCCTCGCCGCAGTAACTCAAGTAGACACCTCAGTCGTTCTCCTGCAGTGTTCTGTGTTCATCACAAGGGGGCAGCCTTGACCCACTTCCCATGGTTGCCAATTCTCCAACCCATCCCCCTCCCCTCATCTAAGAAGACCTTGAACCTGGAGGAGAATATAAATAAACCTGAAAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNATGNGTNTGNNTGGTTAGNGTAAANATGGGGTATGNNNNNNTGGNGNGTTAGANNNANGGTGAGNNTATNTGNATGT SEQUENCE_INCLUDED_REGION=12,456 SEQUENCE_TARGET=343,26 SEQUENCE_TARGET=371,20 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=46.RHAP64FH4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCAGCTAGCCTGGCACACACAACAAAACAAGAACCCTTGCTTGAATAAAATGGAAGGTGAGGTCTGATACCCAAAATTAACCTTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGNTATTTTTATAANATAGAGAAAAGNAATTTCATTAAAAGGAANTGAAAGAAGTNTAAATATAATTATANATTTTAGATTAAAANTTTGTAATNAAAGCACACGGTATGTGTTTATTTAAGTGAGAGTACACGTGGAGTTAAAATGTGGATATANATAAAAATNAGNGAGNTTTTAAAAATNANTTTCTTAGNTTGNTTTAGGGATATNTNTNNACCACAAACANATACANAAAACCCAAAANAAANNNNNNNNNNNNNNNNNAGNA SEQUENCE_INCLUDED_REGION=19,433 SEQUENCE_TARGET=107,18 SEQUENCE_TARGET=126,22 SEQUENCE_TARGET=151,32 SEQUENCE_TARGET=433,15 P3_COMMENT=CL is CCTGGCACACACAACAAAAC P3_COMMENT=CR is TCCACATTTTAACTCCACGTG = SEQUENCE_ID=06.RHAP68FB3.seq SEQUENCE_TEMPLATE=NNNNNNNNACACTTGCTTTTGGTAGGTAAAACGCATGGAATTTGATTCCAAACATGACACAAAAGTTCACCTTATTTCCAACTCATTTCATGCACTTGTGTAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAAACCTTTGGGTTTAGCTACATTTAGTTGGTTCTGTTCCTTGCAACTGAAACATTGAGATTTTTCCCACAAGGAAAGAAAGTAAATGGTGGGA SEQUENCE_INCLUDED_REGION=8,243 SEQUENCE_TARGET=104,52 P3_COMMENT=CL is GCATGGAATTTGATTCCAAA P3_COMMENT=CR is TTGCAAGGAACAGAACCAACT = SEQUENCE_ID=07.RHAP68FC1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNAGGTAGATGATTAGAGAGAACAATCTATTTGGGGTGGATTATTGAGAATGAGAAAACCATTAGAAATGCCAGTAAAACTTCAAATTTGATTACATCTTCATCATCATCATATATTTGTGTGCATGCAAGTGCTCGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCAAAAATCAATAAAATATGCAGTAACTCTTTGTGACTTAGAATTTGGTNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,230 SEQUENCE_TARGET=157,40 P3_COMMENT=CL is TGGGGTGGATTATTGAGAATG P3_COMMENT=CR is TGCATATTTTATTGATTTTTGGCA = SEQUENCE_ID=08.RHAP68FC2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACATGCATAACCCATTCCTGAATCCATCTGCTGTCAAGCACACAACCCCGTGGCAGAAGCCTAGGGACCAGACAGGAAGCTTCTCCTGAGCTTCCCTGTGGCTGTGGCTAGCAGGAGGCCAGAAGGTCAGCATGGTGGTCCTGTGGGGGAGACACTGCAACCCAGCTAAGCTTCAGCTTGACTCCTGTTNNNNNNNN SEQUENCE_INCLUDED_REGION=0,223 SEQUENCE_TARGET=0,32 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=09.RHAP68FC3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGCATACCTTGTCATTCATGTAGAAGTTTGAAGATAACTTGTGAAGTCAGTTCTACTCTTTTACAATGTGGTTTCTAGGTTTCAAACTTAGGTTTTTAGGCTCAGCAACAAGTCCCTTTACTTCCTAAACCATCATACCAGGACCTGTAAAAGAATTGTCTTTCTTCCTGTTTAATTTAAAAAAACATTATTTTATTTCTGTN SEQUENCE_INCLUDED_REGION=9,241 SEQUENCE_TARGET=9,36 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=11.RHAP68FD2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGTATGCACACACACATGCACCATTATGTTTAACTTTATATATTTGTAAATATTGGTTATATCCTGTGAAGAACGTTTATTGCTAAACACTAAAACATAGAAAAAACAAATGAAGTTTGTTTAGATAATTAAGATGAGAAACTCTTTTGGGTAAGATAGGGTGATGGATTCAGGTAAAATGGGAAGCAGGAGTAATCTGG SEQUENCE_INCLUDED_REGION=0,231 SEQUENCE_TARGET=0,28 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=19.RHAP68FG1.seq SEQUENCE_TEMPLATE=NNNNNNATNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAATAATCTGANTGTGTGTGTGAATCNTCTGATGATAATCTANTGNAGATAGTGATGATGTANTACTGAAGATGTGTNATATANACTGATA SEQUENCE_INCLUDED_REGION=6,257 SEQUENCE_TARGET=10,102 SEQUENCE_TARGET=118,52 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=20.RHAP68FG2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNATCTGGTTCCAATGGATACCCATAATGCAAAATATGCCNNNNNNNNNNNNNCCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGGGGCTCTAGGGGATCTTGCCTTTTAAAGTCAAGAGTTTAGTTGGGGGATTCAGTCTTCACTCACCAGAGGACACCATACAAATAAACTCTTCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=16,208 SEQUENCE_TARGET=54,13 SEQUENCE_TARGET=72,52 P3_COMMENT=CL is TGGTTCCAATGGATACCCATA P3_COMMENT=CR is TGAAGACTGAATCCCCCAAC = SEQUENCE_ID=25.RHAP68FA4.seq SEQUENCE_TEMPLATE=NNNNNNNNACGAANNNNNNNNNNNNNNNNNNNNGATGATGANNNNNNNNNNNNNNNNNNNNCATGCTCACTTCTGGGGGCTCAGGATGTCATTCCTTAGGCAATGATCATCTATGNGNTTTTGTTTTTGTTTGATAACCATGTATTCTTACCTAGACCTGTATCCCATGAAAGACATCNTGAGAGAGATCTACAAAAATGTCAACATACATATTACAAAACATAGATAGGNACTCATGAGAAATATATAAANCATTGATAGACACCATACAAATAGACTATAGACAANAGNTANAATAGAGAAGNCACAATGCGACACACACNAT SEQUENCE_INCLUDED_REGION=8,317 SEQUENCE_TARGET=13,20 SEQUENCE_TARGET=41,20 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=27.RHAP68FA6.seq SEQUENCE_TEMPLATE=TNCCACTACTATGTCTATGGAGAACACAACAAATTGAAATAGATCAGAGCTAAGGTTCAGATTGTTCTGATCTGGAATTTGGCATCAGTGTTGAAGTTTTGAATGGCTTAGAATACCCAAGTAAATTTTTAACTAAGAAACTGTTTCAGAAGTTCTCCTAAGATCTGNNNNNNNNNNNNNNNNNNNNGATTGCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGATATTTCCT SEQUENCE_INCLUDED_REGION=0,247 SEQUENCE_TARGET=167,20 SEQUENCE_TARGET=195,38 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=28.RHAP68FB4.seq SEQUENCE_TEMPLATE=NNNNNNNACACACACACACATGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTCCTTCACTCAGGCATCTGTTCTTTGTCAATTTTCCTCCTGAAGGGAAAAGACTTAGCACTATTACTCCTTCTGTTTTAGGAGAGATTTTTATTTTTACTT SEQUENCE_INCLUDED_REGION=7,246 SEQUENCE_TARGET=24,72 SEQUENCE_TARGET=104,46 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=32.RHAP68FC5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNTCTTTATNGCCTGTCTAAGACAGAAACAGTAAAGCTGAAACCTAAAGTCAGGGTAGTCAGGCCTAGAAAGGTGGGAAGCAGACAGTTCAGTAATTATCCATGTGCAGGGAACAGAAGAAATACATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATCNCCGCAGNAGNGTGTGTGTGTGGNNNNGTGTGTGGTNATGT SEQUENCE_INCLUDED_REGION=24,244 SEQUENCE_TARGET=156,29 SEQUENCE_TARGET=193,30 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=33.RHAP68FC6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNACGCTTTATTAGTTCCCGAATATGTTCTATTTTCTTTTTGGTATGAGGATTCCTTCAGTTCTCTTCTGTAGTGAAGGTTTACCACTCACACACTCCTACAGCATATTTTTTATAATGGAAAGTTATTTGAAAAACAGATTTATAATTTTTATTTACCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=16,243 SEQUENCE_TARGET=176,46 SEQUENCE_TARGET=223,36 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=35.RHAP68FD5.seq SEQUENCE_TEMPLATE=NNNNNNACGGTGNCCTTGCCTGAAAATAAAAACAGTAAAGATGGAAATGTAGTTTGACAGTCTGCTTGTCCAATGAGCTTATTAGCTTAAGTTTTATCCCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAGATCAGGGAGAAAGATGTTCAGGGAGAGGGAGGGAGAGAGAAAGCAGGAAAAACGGTGTTCTGATACAATGCTCCTCTCCCCNTCTCTTT SEQUENCE_INCLUDED_REGION=6,250 SEQUENCE_TARGET=103,59 P3_COMMENT=CL is TGACAGTCTGCTTGTCCAATG P3_COMMENT=CR is TTCTCTCTCCCTCCCTCTCC = SEQUENCE_ID=36.RHAP68FD6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNACATATACATACATACATACATACATNNNNNNNNNNNNNNATACATATACACATATACATACACACACATACATATACACATATACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAGTTAGGGAAATAAGATTCAAGGAAGAAGCGATATTATTTTCATATCATAAAACTGGAACAGGCTTAAACCAATAGAGAAATTCAAGTGTCTCTGATTCTAAAGTTCAGTCTT SEQUENCE_INCLUDED_REGION=15,249 SEQUENCE_TARGET=41,14 SEQUENCE_TARGET=111,38 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=41.RHAP68FF5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNAATATATGCACACACATATGCGAAACCCATTTTCACTTCTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNTTTGACTACACAGGTATTTGTCAAATTAAGAATGGCAAAATAAAATGTGATGACCTGCATTTGATTAACAGTTAAGGGAACAATCTATTCTTACAGAGGACTCATATTTTTTTCCATGCTCCAATACAGAGTGGAGCATA SEQUENCE_INCLUDED_REGION=18,242 SEQUENCE_TARGET=60,43 SEQUENCE_TARGET=104,16 P3_COMMENT=CL is GCACACACATATGCGAAACC P3_COMMENT=CR is TCAAATGCAGGTCATCACATT = SEQUENCE_ID=42.RHAP68FF6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNACTCANGATGCTGATCCAATTATCTACAACCTTATAAACATATTTGATAAGTATTCTGAAAAGGCAATCATGAATACACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGTTCCACTGAAAATTTGGAATGAGTTTTTCATTCCTCAAGTTCTTTAGAAATAAAAAATTCTGCTAAGAAAATCATTAGCTCAAAAGACAATGAAAGTAACAAATTATTTAGCTCTTTCAAG SEQUENCE_INCLUDED_REGION=16,245 SEQUENCE_TARGET=98,38 P3_COMMENT=CL is TGCTGATCCAATTATCTACAACC P3_COMMENT=CR is TCCAAATTTTCAGTGGAACATG = SEQUENCE_ID=45.RHAP68FG6.seq SEQUENCE_TEMPLATE=NNNNNNNACANNATACANGTTGCTACTAGGTCCTATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATATGTATATATGTTCAANNNNNNNNNNNNNNNNNNNNNNNCTTTTAGCTTATTTTTATGTGACTCCTGGCTATGAAAAGGAGCGGGTGCTGACACTGGTTGTAGCTCTTGTGACACTTTTCCTCCTGTTGGGGTT SEQUENCE_INCLUDED_REGION=7,240 SEQUENCE_TARGET=39,67 SEQUENCE_TARGET=129,23 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=48.RHAP68FH6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNTTATGTATTTAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACAATCATGTGCTGTGTTATAACAACAAAGAGTAAATAAAACGGCCATGAATTTGAGAGAGAGCATGGAGGGTTTATGGGAGGGAGGAAAGGAAAAGCTCATATGATATAATTTTAGAAAGAGAAAAAAAATCAGGAAACACATGTATTCAGTTTTCCCAAAGACTCTGAAGCGTAACACTAGTTTATGCAGCAGAATG SEQUENCE_INCLUDED_REGION=17,246 SEQUENCE_TARGET=31,32 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=01.RHAP69FA7.seq SEQUENCE_TEMPLATE=TGNGNTACGTTNCNCTGNTTTGGCCGTTTTTTGCCNATCCGGNGTCACACTGTTTGAGCCACGGATATCCCAGTCAGCTGTTTCCTACCTTTCCACATAAGGGAGAAATCTTAGTCCTTATGTGGGGCCAAGAATACATGGGGTGGGGGTGGGGGCATAAACAGGTTTTGCTTCTTGCGCTATATGGAATGGAAAGAACCAGACTACAGAGCGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCCTANACAAATTGGGGAGGGGGGTNTAGAAACCCAGGGCAAGCAGATCCCAGGAGCACCGAGNAGGC SEQUENCE_INCLUDED_REGION=0,326 SEQUENCE_TARGET=217,40 P3_COMMENT=CL is AGGTTTTGCTTCTTGCGCTA P3_COMMENT=CR is GTGCTCCTGGGATCTGCTT = SEQUENCE_ID=03.RHAP69FA9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNACGTTCTCCTGTAGGACAAAGACTTTCTGTGGTTACGACGGATATTTCTCAGACTTTATCATTAAAAAGAACTGACATACAGCTGCGGCTGTAGTTCACATGTAGGATATTTGCTGAGCATGGATGAAACTCTGGGTTTGAGTCCCACCATCAGAAAAATGTGCTTCCCATTTATATGGAGAGTTATTAAATTTGTTCTCACAGTTTGATCCATTTCTCCTCAATATAAAGATCTATGAAGGAATAAGAAGGGAGAGTTCTGCTTTGATGTTAATTAGCAAGAAAGACGACATAGCAAGAAACAGAGCTGAGTCCAGATTCCAGCACATGTGCGCTNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,350 SEQUENCE_TARGET=356,14 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=04.RHAP69FB1.seq SEQUENCE_TEMPLATE=CNGNATTATNTCCANAAANAAATTATTTNATTGCAACANAAAACATTACATGTCAGCCTTAAAAAGCAATTAAATCAAAAAAACAAAAATACCAATTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCATCTCAAGACTCTGTTTCTGTTGTGGGGCCTGTGGGGTCTGGAGCTCCTTTGAAACGGAGACTTGGAAATTGCTCACCTGGCTGAGCTTTGGCCCCTCAGAGGTGGGACTTTCTTTACTAACAATAGGAAGCTGGAGAAGCTGTCAAGTGCGAATGGAGGAGACCTGCTCAGAAAGGGAGGGGAGCTGTGAGAATTTGGTTTCTCTCAGTATTGGGGTGGGGGTGGGGTCTGGATTTACTTGTGCCTAAGATTCCTTTAAAACTCTGACCCGATGCA SEQUENCE_INCLUDED_REGION=0,435 SEQUENCE_TARGET=100,52 P3_COMMENT=CL is CATGTCAGCCTTAAAAAGCAA P3_COMMENT=CR is TCTCCGTTTCAAAGGAGCTC = SEQUENCE_ID=09.RHAP69FC9.seq SEQUENCE_TEMPLATE=NNNNCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGGTTAGTTAAGAAGACAGTTAAAGATGGATTGAAAGTAAGCATTGTGGTTTGTGGTTAGACCTTGTCAAACTGTTATCCAGATAAGTCATAAAATATTTCAATGGTTCGTTTGAACCGATATAAATTCTTTCTAAAGAAATACTATAGTATTGCATTAGCAATATGGCACTGTGATAAATTAGAAAAGAGATTAAGTGTTCACACCTCGGATTGTTGTTTTCTCACTAAGCAATTATGTTAATTCAGAACTATGCAAAGCAAGGAGGAAAGAAAATAAGAAGCCACAAAGA SEQUENCE_INCLUDED_REGION=4,350 SEQUENCE_TARGET=9,52 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=10.RHAP69FD1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNACTTTTTAAAAAACCAAAAATCAGGGGAGATGGCTCACACAGCAAAGGGCCAGATGTGTGAGCTTAAGGTTCGAGGCACTCCTGTCCAGTGTCTTCTGAGATTATTACAGTTCTTGGGAAGTAAAGACATGAAGATGACTGGACCAAACTTGTCAGCCCACTCTAGCAAAATAAGACTGCTAGGTTCAATGAAAAACCCTATCTAAAAAAATAAGGAAAGTGATGAAGAGTGAAGACACCTGATGTTAGCTTTTGACCTCCAAGTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCATACATACATGCACACACATGTGCATGCACATACAAACAATCATGCACACAGACATAAGCTCTAGAAATTACCATCAGTAATGCAACAGACTTTTTAAAGACTGGTATTCTAATAGTAAGTNNNNNNNNN SEQUENCE_INCLUDED_REGION=16,443 SEQUENCE_TARGET=284,50 P3_COMMENT=CL is TGCTAGGTTCAATGAAAAACCC P3_COMMENT=CR is ATGCACATGTGTGTGCATGT = SEQUENCE_ID=13.RHAP69FE1.seq SEQUENCE_TEMPLATE=AAACAAACATAAAGTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCAGAAAAATGTTCCTGCAGTGATGTGGATGAAGGATAGGGATGGATTGAAGACGCCAGCAGGCTGGCTTTGTGAGAGTCCAAATAAAACCCTGACAGCAGAGGCATGAGAAGGATTCCAGAAATTTTTAGAACATCACAGCCCAGGCTTTGGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,233 SEQUENCE_TARGET=20,56 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=15.RHAP69FE3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNACACAATTCATTTAAACCACATGCTAGAGTCTACCCTTACAAAAAAAGCAAAAAAGCAAAATATTATAACTTAATTTTTTGATTTCTCTCTTCTATATCATGTCCTACTCTCTCCTTTGTAGATACCATCTCTACCACCTGGTAACACAGCATTTCTTTTTTTCTCTTTCTACATGAATATATCTAGACGACTCAGACACTCATCTACATAAAGAGAAAAATAAGCCAAGACCCTCCCTCCCTCATATGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTTCCATGAGTGTGAAAATGTCCCTGTGGGGAAAATAGCCAGTAGTAGCCAGTATGCAAATTTAAAAAGAAAAGGTTCAGTTCTTCATTCTTCAATTCCTGTCTCACATCATCTTTGTTGCCTTAAGGACAATGAAAGAATCAAAATATCTGCTAGAAACTTTCCTTGGT SEQUENCE_INCLUDED_REGION=17,446 SEQUENCE_TARGET=272,22 P3_COMMENT=CL is CCACCTGGTAACACAGCATTT P3_COMMENT=CR is CCACAGGGACATTTTCACACT = SEQUENCE_ID=21.RHAP69FG3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCAGCCATTCACAGTGCCTTCTCTGTCTCCTGCAGTCAGTTCAGGATGTAAGCCCTCAGCTACTGCAATGATCCCCAAATGAGAAATGAAATGTCTTAGTGAACTTTTTTTTTCAAAAAGTTAGGTATGCTTAGTGGTAGGAAGATGGCTCATTTGATAAAATGCTTGCCCCACACTCAAGCGTGATGATCTGTGTTTGGTTCTCTTATACCTATGATTAATAACAAAACCAAGAGTGGGGAGATCCTGCCTATTATCCCAACACTAGGAAGGCACAAATAGGAAGATCCCTGAAGCCTACCAACCTACCGTAGTCATTGAGCTCCAGGTTCAGGGAGAGACTCAAAAAATAAGATAGAGAATTGTAGAGAAAAATGTAAGCCCTCAACCTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAG SEQUENCE_INCLUDED_REGION=31,446 SEQUENCE_TARGET=427,48 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=25.RHAP69FA4.seq SEQUENCE_TEMPLATE=NNNNNNCCTATAATGTAGGCACTGTTCTGTTTTACAAGTGACACAATTATTTCCTTGGCAGGCACAGAGCCTGAGTGTCCCCTCCAATCCCCCACACCAGGGCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCATCTCCTACAAGGTGTGTGTGAACGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=6,175 SEQUENCE_TARGET=106,44 P3_COMMENT=CL is AATTATTTCCTTGGCAGGCA P3_COMMENT=CR is CCACGTTCACACACACCTTG = SEQUENCE_ID=26.RHAP69FA11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNACTTATTTACAGAGACTTTTAAGGGTGACAGAAGCCTCTGAGTTAAGCCAGTGGGCACTATGTGATTTTCACATCTCCCAGCAGCTCCAGCATTGCTGTGAACTAGGGAGTTGACCAGTCTTCAAGTGATGCTGCTCTCAGAAACATTAGTCAAGAGGGTAGTTTTAAATTGCTGTTTGATTGGTTNNNNNNNNNNNNNNNNNNNTNCCGNCNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTAAATTAGCTAATCTAGAAACTCACACCTTGTTAAAGTGCAGAGACTAAGTGACCAAGAAGAATGAGTCACAAAGGGGACATCC SEQUENCE_INCLUDED_REGION=18,353 SEQUENCE_TARGET=204,18 SEQUENCE_TARGET=231,18 SEQUENCE_TARGET=250,34 P3_COMMENT=CL is TTTTAAATTGCTGTTTGATTGGTT P3_COMMENT=CR is TCTGCACTTTAACAAGGTGTGA = SEQUENCE_ID=27.RHAP69FA6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTATACTTCCTTTCAATTTTTAGCACTTCNAGTCATACATAGGACATAGTCACACATTTCCATTATGCTATAAAATATTGTCAAAATTTAAATAAGTCATCATGGTTGAAGTTATTTCATTTGCTATCTCTAAGATTAGAGCATGCTCTAATATCTTCCTGGTTCCAGAAAATTCAGACCTGTTTTTATCTTATTAAATACCCTCAAGCTCAGCTCATTCTTGAACTGTTGAACTGCAGCCAAGGTGAAGACATTCACATACAAATTAAACATTTTATTTTGCTAATGTTTTTGTGACTGCTTACTACTCAGCAGTAGCTCCTCAAACCAAAAACATTTGATGAGCCATGACTTTGTCATAAATAAGAAAAC SEQUENCE_INCLUDED_REGION=19,447 SEQUENCE_TARGET=23,69 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=31.RHAP69FC10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNACTTAATNTGTTTTTATGATATATTGAATCTCGGTCTCATAATGAGACAGAACATGTGACATTTATTTTCTTCCCCAGTTATTCTATCTTGTTCCCAGTTCTCTTCCTTTGAACAATCAGTCTCCCTTTACAATCTTTTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCAGAATACACCNAGAAATTTCAGTCCGGTGNGCGGTTGATTTCTGGGTATGTCCATATATGTANGTATGTGTATGTGAGTGTCTAGAGAGTTCACAAGAATGCATAGGCTCTTGGGGCTGAAGCTACAGGAAGTTCTCACTTCTCCAATTTGAGTGCTAAGGCATGGA SEQUENCE_INCLUDED_REGION=18,354 SEQUENCE_TARGET=160,42 P3_COMMENT=CL is TCCCAGTTCTCTTCCTTTGAA P3_COMMENT=CR is CATACCCAGAAATCAACCGC = SEQUENCE_ID=32.RHAP69FC11.seq SEQUENCE_TEMPLATE=ANANTAAAGTCCCANCNNNTGTGGGNNGGAGGGTATCCTGGTTACATCAAACCTCTGCTGACTGAGGGCAATAGGGGCAGTATCAGAGAGGCCAAGAAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGGGGGTGGAGGGTGCAGGATGATCCATGAGGGGAATCAGTTGTTGCTTCTGGAAAGATTGGGGTAACAGTCCAGAATATTCATATAGGCAGCAGCCCTGGTGGTTAGTGATGCTGGGATTTAAATCAGGGCTTCAACAGTGTTCGAAAATGTGAGGAAGGCGTCTGTTTCTCACCTCTGATCCTTATTATCAAGTCAGGAGAACTTT SEQUENCE_INCLUDED_REGION=0,362 SEQUENCE_TARGET=101,52 P3_COMMENT=CL is AGGGCAATAGGGGCAGTATC P3_COMMENT=CR is TGATTCCCCTCATGGATCAT = SEQUENCE_ID=33.RHAP69FC12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNGCCTCCCAAGTCCCCTCAGCCTCCTCACCACCTTTTATCAGATGGAGTGGAGGAGATGGCAGTCTCTCCCTCCCAGAAGGCTTTGCAGGCTGCTGAAGCCCTTTCCTTCAGGCTGAGCAGCAGCAGCAGCAGCCCCCAACTGGGGCTGTATTATCATTCCATCTTGACCTCAAAGGAAAGAAAGTGAAGCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCACTCACATACTCACACGCGCGCGCNNNNNNNNNNNNNNNNCCCTGTCACCCCAGGCCTCTTATCTTGCTGAATCTGCACCATCTCTCATGAAAGAGGCATGCTT SEQUENCE_INCLUDED_REGION=19,357 SEQUENCE_TARGET=212,54 SEQUENCE_TARGET=296,16 P3_COMMENT=CL is CTTTCCTTCAGGCTGAGCAG P3_COMMENT=CR is GCGCGTGTGAGTATGTGAGT = SEQUENCE_ID=33.RHAP69FC6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTTCTTTGTCCTGTGGCTCAGTTCAAACCATTCATCTCAGAGAAAAAAGCAGAATGGTATATGATAGGCTTGAAAAGTGGGACTTAGAGGATGAATAAGGAGAATAAAGGAATAGAAGACCATACTTAGGCGGAATAACTTCTAGTGTTCAATATGGTGTCTGACACTAATTAAGTGTATATTATATTGCCTATATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAGCCACTTACAGAGAACTGAAGACATGAGGCAGAGTGAAAGGTCAAAACATTTGCAGATGAAGCAACCTTGACCCAGTGACTGCAGACTCACTTTTGTTCTATCCCATCCAGTTCCTCAAAACTCAGACGGCCTGACACTCACTAGCTAAAAATAACATGATCTTTTGCAAAGGAAATATTTAAGGCTTCCTATTA SEQUENCE_INCLUDED_REGION=31,449 SEQUENCE_TARGET=231,50 P3_COMMENT=CL is GGCGGAATAACTTCTAGTGTTCA P3_COMMENT=CR is TGACCTTTCACTCTGCCTCA = SEQUENCE_ID=35.RHAP69FD11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTTGCTCTTACATGTCTGACCTGCTTGTCCTGGTGGTTAGAACCACTTCACATTGTTATCTTACAGTTTTATTGATGGGTTGCCCATTTGCTCATTCTTTGGGACAGAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCCCTCTTCCTCACTGGCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,173 SEQUENCE_TARGET=133,34 P3_COMMENT=CL is TGTCTGACCTGCTTGTCCTG P3_COMMENT=CR is ACCCAGCCAGTGAGGAAGAG = SEQUENCE_ID=39.RHAP69FE6.seq SEQUENCE_TEMPLATE=CNCTNNNNNNNNNNNNNNNNNCTCTGNACACATTCGGACACANATTNTTNACACACNTTTTCTGTGACACACATACNCAGNNAGATACACACNTAACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCACNGACTGTCAANNNNNNNNNNNNNNNNNNNNNNCTATACNGACGNNNNNNNNNNNNNNCTACAAACGGGACATAAAGCGGGACACGATGANAGGCTTTTCCNCNCACACNAAGTGATANCNNACAGCNTCNCACAGACANGNNNNCGNAGTGNTTGANNCNGACGCGCNCTCNTNANNGGAGGCACGGTCTAGNNNGCGCAGGTCTNGAAGGTCGGNTTAGNGNTGNNNNTANNGNNGCNCTTACNAACGCNGANTCATANTNTNTCAGANNNNGAGGGTNTNTTATNTAAAGNNTCAANANAAANAA SEQUENCE_INCLUDED_REGION=0,442 SEQUENCE_TARGET=5,16 SEQUENCE_TARGET=99,32 SEQUENCE_TARGET=145,22 SEQUENCE_TARGET=178,14 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=40.RHAP69FF10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACTNNTAAAAAATTGTCTTCAATATCTTTTTAAACCAATTAAATTTTTTAATTAAAATATAATTACATCACATCCTTTTTCCCTTTTGTCCCTCCAATCCCTCCCATCCTTTTCCATCACTTTCTTCCAAATTGATGGCCTCGTTTTCTTTGATGATTATTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACGCATGCACGCGCGCGCGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCAACCTTCTATATCCATTTAGTGCTGCTTGTATATGTTTTCACAGTAGAGCACTTTGTATTGGATAACCAATTAGGTTGCTCATCCCTG SEQUENCE_INCLUDED_REGION=19,361 SEQUENCE_TARGET=183,40 SEQUENCE_TARGET=248,40 P3_COMMENT=CL is CAAATTGATGGCCTCGTTTT P3_COMMENT=CR is GCAGCACTAAATGGATATAGAAGG = SEQUENCE_ID=40.RHAP69FF4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCACGCACACATCTGCATACTNNNNNNNNNNNNNNNNNNNNNNNNGACATACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAATGGGCCCTTTGGCTTTTTTCTGATGACCTATCACCATCTCAAGTTCAAGACCCTGCACAGACCAGGTAATTTAAGAATCATCACCTTAAATTCAAGTTGCACCTTATGATTTAATTATTTCATGGAGATGGCTTTCTCCTTTCCAGTTTTATTCTCCATAAACTGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=17,308 SEQUENCE_TARGET=17,21 SEQUENCE_TARGET=60,24 SEQUENCE_TARGET=93,60 P3_COMMENT=CL is GCACGCACACATCTGCATAC P3_COMMENT=CR is TGCAGGGTCTTGAACTTGAG = SEQUENCE_ID=41.RHAP69FF11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNTAAGAGCTTAACAACTTATGGCAGCTTTTTAAAATCTGTCCACTTTTCTAAGTGCACTGTCTTCTTTGTCTCCCTTCTATCGTAGTTTTAAGCACATTTTCCTTCACACTTTGAGTTTTCNNNNNNNNNNNNNNNCATGACTCCTCTGCCATTATGTGACTGTTGTCCACTATGTGGCTGACCTCAATGTGGGGTTAACTCACATGACATGATTTTTTCCCCCTTTTCTCCCCTTCATCTTCCTTCTCTAGGCACCTGCTGAGATTTACAGCCTTGGGGATATTTTTTAAGCCTGAAAAGNNNNNNNNNNNNNNNNNNNNACAGNNNNNNNNNNNNNNNNNGNCANNNCGCTACNGNGNG SEQUENCE_INCLUDED_REGION=18,360 SEQUENCE_TARGET=138,15 SEQUENCE_TARGET=318,20 SEQUENCE_TARGET=342,17 P3_COMMENT=CL is GCACTGTCTTCTTTGTCTCCC P3_COMMENT=CR is TGAGGTCAGCCACATAGTGG = SEQUENCE_ID=42.RHAP69FF6.seq SEQUENCE_TEMPLATE=CACTNNNNNNNNNNNNNNNNCACTGNCACACGNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCAAACCAGACAGAGAGTTGACACATTCACAAGTTCAAGACGCAAAGTGTTCTTTTCTTCGAATTACACTTCCTTGAGTTTCGCCTGGCCTTGGGTTGTTTTGAAGTTGGCAAGTCTCCTCAAAGCTGCTTTTGAGAGCCACCGTGTTCATTAGCTGGTTCACAGCTTCCTCCTACCACTTAGCTTGCCTGGAACCCTGCTGTGCAAACAGGCCTGGTGAGCTGGGATGTCTGGCACGTGCAAGGCCCAGGTTCCACCCTGGCACAGTGAGGAGGAAGCAGGCCTTGTGTGTGGACACCCAGTCCCTGTGGCACTTAGAGCCTACCNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,428 SEQUENCE_TARGET=4,16 SEQUENCE_TARGET=35,40 SEQUENCE_TARGET=402,26 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=44.RHAP69FG5.seq SEQUENCE_TEMPLATE=AANTNTATNNTTGNNAGGNNNANGNANGGAGNNNNNNNGGANNGNNNNNTANNGTNNAANNNGNTNGGGNNNTNGGNNNNANNNACNNNNTCTNAANNTCTATTNANTNCCACNTNNTGGGGNNAACCATNCANTNNCAGNTGTGCTTNNGAGGGTGGNNTTCTGATTTNAAAACCAANCAACANNAANNNATTAGANANNNNNNNTNNCACAACACNCATNANNCNNNNNNNNNNNNNNNNNNNACNCNNAAACTCCANTCACNCANAAATTATTCTTCTTCNCCAGCNANTTCATGAAANTTTTCTTCAAGAACCACATTACTTTGGGCACAAAAATAAGGCTCNTCAGGATGACAAAGAAAAATTGNACTAGGNACTGGNTTAAAAGAANAGCATAATTCAAACAATATAATGNTGGGNAAAANTGAATGGGCTGACAGATAGAAGAACTTGATCANCANGGAACANNATTTNAACTTCAAGTTAATNAAAAACNC SEQUENCE_INCLUDED_REGION=0,499 SEQUENCE_TARGET=228,14 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=01.RHAP131FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCNTTAAGGATTACAANACTGAACCACCAACCAANNAGCATGCATNGGNNTGACAANGNCNNNNNNNNNNNNNNNACACNCTCGNCNCNCTCNCACNCACNCNCTCTCNNNCNTCTNTATNTAGNGNGGTATANTTGNGTCNCTAAANNTAGAATGTTNNNTNNGNNATNTTTNNANANGGGGGNNGNTNACCTACANGNNCNCTNTTNGCTAACNNTTTTGTTATTGCCANTCAACTNNAANNTTTGGGNGNCNNNNTTNTTGNTCTAGANTTTTNAATGTNNANAAAAANANNNAANNTNNTAAGANTNNNNTNNGGTNNNNTNNTAAAAGGGNGTTTTTCNNCNAATAANANNGGGGNAANANNNNAGGGGGAGNNNNTNANAAAANNNGGGGNTNNTTGNANNNNNNAATANNNATAGNGTTNAANNNNAATNNNNTTCAATTNNNAAATGNNAAGNGTTNNAAAAAAANTGTTNNTNNNTTNNTTNAAANTGATGANTNTGNNTANNACNAANTTTCTNNGNNGAGAANNCCGAAGCCTCCNAAANNCTNTNAATTNGNNNAANCAANAANNNGNNGGCNNAAANAAANNATTGTTNNNCCCNNNNAAAATTNCTCNNCCNNNAANNANGACGNNNNNANNATNNNNGGNNANATNCNNTGGGGNAGCNTNAATTNNATGANNTTNNNNNACAAANNAATTNGANNANNCTA SEQUENCE_INCLUDED_REGION=23,717 SEQUENCE_TARGET=84,14 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=01.RHAP131FA7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCAGTATAAGTATGTCTGGAGATCTTTTAGAAATGAGGGGTAAATTCTGAGGGCAGGAAGGTTTTAGGGGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACTGCCTGCAAATTGGTATAGGCTGAGAAATTCAATTAATCGTATTCATCAATTTTTCACTCTGGGGGGGGAAATGAGGCAGAGATGTGGAATTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,224 SEQUENCE_TARGET=95,50 P3_COMMENT=CL is GAGGGCAGGAAGGTTTTAGG P3_COMMENT=CR is CCCCCAGAGTGAAAAATTGA = SEQUENCE_ID=02.RHAP131FA8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCAGTATAAGTATGTCTGGANTAATAATAGCAAAAAGTATAAAACCATGAGGGCAGGAAGGGTGGCTCCACTGGGATTACCTGCTGGAACCTCCTCCCTTTCCACAGGAACCTGAGCTTGTGACAAACTAATAAAATATGGAATTACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCAGAAGAACAGGGAGATCTTGGAGGACCCATTCCAAGCATTGTTTATTCATTCATGTGGACACCGTGACTTGGGGGCTGGAGAGAGGGTGCAGCAGTTGAGAATAGACAACTCTTCCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=22,336 SEQUENCE_TARGET=170,61 P3_COMMENT=CL is TTCCACAGGAACCTGAGCTT P3_COMMENT=CR is AACAATGCTTGGAATGGGTC = SEQUENCE_ID=04.RHAP131FB7.seq SEQUENCE_TEMPLATE=TCTAGGACCCCCCTATTTGGGCTCTTCTCNCCGGGTCCTCTGAAAGAACAGCCAGTCTTCCTTTACACTGGAAAGCAGAGAGTGAGTCATTTAGAGGGTCTTATGACCTGCTTCCTGGGAAAGTCAAGATGTTCTTCCTATTTTTAATGATCTACTTTAAATAGGGAAAAATACAAGGACAGAGAAGATTATTTTTGCTTGTGACATTTCCTTCACCAAATTCCTGCAGCTTAAAATACTAGTGTGCCAAAGTATGGTAAAATGTTCTTGAGTTCATCAATACAACTATTTTCTTAAAAGAATTCTTTATCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGGGGTGAGCGAGCAGTCTCTCTGTGCATGGAAGAATTGGGGGATTGAACCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,422 SEQUENCE_TARGET=313,50 P3_COMMENT=CL is CCAAATTCCTGCAGCTTAAAA P3_COMMENT=CR is AGAGAGACTGCTCGCTCACC = SEQUENCE_ID=08.RHAP131FC2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCAGGATATTTTGCCCTTGCAAGGAACATACTACACAGCTCATGCCAAGTTTTTCATGGTTTTTGTGAGAAAATTTTAGGTGCAGCAAAAGAGACGATTGGTTACATTCTCACAGCTGATTAGCTCCAACACAGAGTGGTATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGGGTGGGAAGAGAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=22,210 SEQUENCE_TARGET=167,46 P3_COMMENT=CL is TGCAGCAAAAGAGACGATTG P3_COMMENT=CR is CCTTCTCTTCCCACCCATC = SEQUENCE_ID=08.RHAP131FC8.seq SEQUENCE_TEMPLATE=AAAAANNGNTTAGAGGATCCCCCATAGAAGCTTCTACTATACCAGCTCTAGTAGAAGGGGACAAGCCTTAGTTTATGCAACTGAGTTCATGGCACGGGATTGTAAATGGTACATGAGTCTTATGTTCAAGCATTATTTCTATAAAATCTCACTTTTTAAGTCCAGTGAGTAAGCTGTGAGCTCCGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTCCCAGCTACTTGGGAGGCTCAGTTCCATGATGTTTATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,293 SEQUENCE_TARGET=187,64 P3_COMMENT=CL is TCCAGTGAGTAAGCTGTGAGCT P3_COMMENT=CR is CATCATGGAACTGAGCCTCC = SEQUENCE_ID=10.RHAP131FD1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNGAACTATATCCCCAGCTCTTAGGAATGGAAATTCAGGATTCCAAAAGGAAGCAAAAAATAAAGCCTCACCCATAACCCCACATTCTTTGTGCACGCCCCTCTCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGTGAGAATTGAACCCGGAGTCTGTCTAAGCTAAGTGNNNNNNNNNNNNNNGAGCCACCGCTAGCCACAGCGTTGTTTAATTACCTGTTGCTCTAGTTCAGAACTTTTACTGTGGTTAACCATGTTGAGCTACAGTGTTTCACTGTAACCACTTCTCATGGCTCGCTCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,326 SEQUENCE_TARGET=118,46 SEQUENCE_TARGET=203,14 P3_COMMENT=CL is CAGGATTCCAAAAGGAAGCA P3_COMMENT=CR is GACTCCGGGTTCAATTCTCA = SEQUENCE_ID=10.RHAP131FD7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCACTACTGCAACAGTGTTTCCACTTCAGCATGTGTGGGTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATATGGGTATCTCTATCTGTATCTGTGTATCTCCTTTATTTATTGATGACTTTAGATGCAGATTTTTTAGGGTGGAGGATGTCCTTGTTTTGATCACATGTTATTTGAAGAACACAATTCCCAGCTAAAAGAATTTAGCTATTTTAGTTGTATCTGCAATGAAAATGCAACTTTTCAGTGAAATTAGCCACGGGTTGAGTTTATGATGAGATGCTCTTTGGAAGACAGAAATGTACTCTCATTTATAAGTTCAAGTCTCATTGAGAATACTCCAAGATTTATTTGTTTACTTTTCAAGAAAAAAAATTGATTTACAGTCTCGAACACAAGAACAAAGCCACAGACAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=12,433 SEQUENCE_TARGET=53,42 P3_COMMENT=CL is TTCCACTTCAGCATGTGTGG P3_COMMENT=CR is CAAGGACATCCTCCACCCTA = SEQUENCE_ID=11.RHAP131FD2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCAACCATCAACCGAAGCACAAGAACAGACGTGCGTCTTTTGGCTCGCAGTGATTTTTAATGGTAGTCATCCGGACATACGACTTAGATACAATGAGACTACAAACTAACAGACAAGATTCGGGCAAAGCAGAAAAACAATGAAGCCAGCATCCTGATCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGCATGCCTGCCTGTCAATTGTGAGGTAAGACAGGAAGACTTGGTCTCCTGCCAGATCTCCATATCTTCCCGCTCTCCTTTAACTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=22,297 SEQUENCE_TARGET=183,45 P3_COMMENT=CL is AAACAATGAAGCCAGCATCC P3_COMMENT=CR is TCTGGCAGGAGACCAAGTCT = SEQUENCE_ID=13.RHAP131FE1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCATGCCTGGAAGCCACATTGACTTTATATTGAAATAATTCTTTTTTAAAAAGTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCGCATGTGTGCTCATGCCCTTGTGTGCATGATAAGTGTATGTAGGCTTTTGTGCCACAGCAAATGTGTAGAAGTCAAAGGAAAACATTCTGAAACGAGTTCTATCTACTTTTCTGTGGATTCCAGGGACTGAACCTGTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,241 SEQUENCE_TARGET=77,38 P3_COMMENT=CL is CCTGGAAGCCACATTGACTT P3_COMMENT=CR is TATCATGCACACAAGGGCAT = SEQUENCE_ID=13.RHAP131FE7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCTTTTCAAATGTGGAGCCTACTCTAACTTTCAGTCCCTGGGTCTCTCTCAAATATTTTCNNNNNNNNNNNNNNNNNNNNNNGTTTTTNNNNNNNNNNNNNNNNNNNNNNNGNNTNNNNCTCTCNNNNNNNCNCNCNNNNNNNNNANNTCNCTCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANATAGNNNAAANAANCAANAGANCCCNAGAAAAAGTGCACATCNATGCCAACATACAGNAAAAAAAACATTTNGACACACANAAAGAATANAAAACANANNTGTGNNNNATCAANGGTATTTTNGAACCNAACACGAATGNGTGANCANNGNNCANNANCGNTGCTCCNNTGTGAAATTGTTANCAGCTCANTNTTCCTGCNGTAATAATANNTACCCGCAGAATNNTNTACAAAACATGGGGGNCGNAAACATATGNGTNTTACANNCNGGNGTGGCTNNNGNGTGAGCTNACANANNNNNGGNGGGGNAGCGCTCTCNGGNCANCTGCCTNNNGGNANNNNCANCGCGCCNGNANATGNAGNANNCGAANNCGCGNNGGNANNNNNTGTCTNNNAANAGGNANAAGGGGANNNCTTNTTTCCCANAGNGGCNGGCNNNANANNTNNNNNACCGGCCACACTGANNTCCCAAANNNC SEQUENCE_INCLUDED_REGION=27,677 SEQUENCE_TARGET=90,22 SEQUENCE_TARGET=118,22 SEQUENCE_TARGET=189,36 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=14.RHAP131FE8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNCCAGGAACCCAGCCAGTTTTCAAAGAATACTTACCATGCCATACAAGTCAGAACCTGAACCTAGGCTTTCAAGCTGTGTAAATCAATGTTCAATTGACTGTAGTAGGAGACCCACAGACTCTATTAGAAGCATTGTAGGACAATGCTAGATTTTCTGTTCTAATGTTCTTCAGATTTAGAACAACGATGACAACAGCATTAGTGTAATAGTGTTAACTGAGTACTGTAGTGCAAGAGTGCCTGCATTCCCACATACAGTATTCATAGCTTTTGGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCCAGTTTAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=25,321 SEQUENCE_TARGET=301,32 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=15.RHAP131FE3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCTGTTTGACAGCAGGCAGAAGCTCAGGAACAATTTGTGTGGGAGTCCCAGAGGGGAGCAGGGTGACTGAAAATCCTCTGGGCTGTTACAGAACGGAGTTTGGAATCAAAGTAACCAGAACATTCAGCTCAGTAAGAGCTTAAAAGGACATTTCATCCCCCTTAAAGACAGCTAATAAAAATAGATTTGCAGTCAGTTTTCATTAGCCAAAGTGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCCGCGTGCATAGGGTGTCTTCCTCAGCTGCTCTGCTCACCTTATGTTTTGAGACAGTGTCTGTCTTTGAACCTGTAGTTCAGCTTTTTCTACTAGGCTGGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=12,368 SEQUENCE_TARGET=229,38 P3_COMMENT=CL is AAAAGGACATTTCATCCCCC P3_COMMENT=CR is AGGAAGACACCCTATGCACG = SEQUENCE_ID=15.RHAP131FE9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNCCTTTCCATTGTGAGACACTCATTGTTGGACTAGCCCAACCATAGCCTGCAAGCCATTCTAATAAAAATTGCTTTAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCATGAGCAGGAGGATGGAAACATGATGCCCTTAATTCAATGAAGTCTAAACAATATATAGACAGTTGCTATCAAGCTCTAGGAAAACAATCAGCACGACTTATTTGGGAATAAATAGATCACAAGAGTCTCTTGAGTCAACTTACAAGAGTCCCTAGCCCATGTTTCATGGATGCTGCACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=26,309 SEQUENCE_TARGET=104,46 P3_COMMENT=CL is TGGACTAGCCCAACCATAGC P3_COMMENT=CR is AAGGGCATCATGTTTCCATC = SEQUENCE_ID=16.RHAP131FF1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCCACGAATCAAAGAAAAGGCAAACTAGGGAAAGAAAAGCTAGTCTCAAACACCTGACCAATGGAAACCTTAGAAAGAGTGAGAGCCAAAATGATGCAGTTCCCAGTACTCCCTTATTGAAGATGATCTAGTTCCAAGCACTCACTACTCATAAATACAGTACCTGGTTCTCCCCTATCCAATGTAATACAGTACTTAGTACTCCCCCACCCAGTGATATAGTGCTTAGTACTCAGCCATGATGAGAGTACCTAACACTTCCCCCACCCAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGNGGGGGTGNNTNTGTNNGNNTCNGGGGGTNNNGCGCGCNNNNNNGNGNAGCATGGNGNNANNNGTCTNNTGATGTGAAANNNTATNNNCGCACAATNNCTGCANAACANANGAGNNGGNGNGNNAAATGNGGTGAAANCNGNGGNGCCNANTGAGNGAGNNNCACACANTAANANNGNGGNGCNNNNNGGCGCNNTGNANNNGGGGNANNNNNNGTGNNANCTGNACTGNNNGNNNGGCNTNNNGGNNGGNG SEQUENCE_INCLUDED_REGION=11,569 SEQUENCE_TARGET=283,40 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=16.RHAP131FF7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCCCTTAAATGCATCACGAGAATTCTCACTTGAGATGATTTCAATTCTAGCTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTGTTCCGGCGCGCACACACGCACATATACAGCAAAATATAACTGAATGCCAAAAAGCATTGTCATGTCCTGTGAAGGGCACAATTGTAATCACACAGGTATGAAAAGCTTAAAATCCTTCCATTACCTTCCATGCCTGAACACTGACAGGGTCGGGCTCAGGAGCAGAGCCCACTTCCTGAGCTATTTATTTGTATCTCTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=12,305 SEQUENCE_TARGET=66,44 P3_COMMENT=CL is CCCCTTAAATGCATCACGAG P3_COMMENT=CR is TGTATATGTGCGTGTGTGCG = SEQUENCE_ID=18.RHAP131FF9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNCCGGTTGCATGCATGTACCCGCCCAGGAGCTAAGTCTGATTTTCGGATAAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTAGCTCATTTGAAAACTAGCTTATTGGCTCTGAGGCTGGGCTCTTCTAAGCCTTCCACAGCTAGTGTGCCCTGAGCTTCACTCCTAGCTCAATCCCTCAGTTTAGTTGCCTAGTTACCTTCTGTGAAGCCCATTGGTCTTGTTACCCAAGATGCTTTGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=16,274 SEQUENCE_TARGET=69,58 P3_COMMENT=CL is GCCCAGGAGCTAAGTCTGATT P3_COMMENT=CR is CCAGCCTCAGAGCCAATAAG = SEQUENCE_ID=19.RHAP131FG1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCCAGGATTGATTGACGCCTTGTGATGATGTCCTCTTCTAACCCTGCTCTTATACAAGGTGGAGTAGCTGGTATGTTTGAATGTTTAAATAAAAGGTACCCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTGCTTGGTATAGGTGCTTATAACTGTATCTCAGGTCCTCTGGGTTTGACAGTCAACATAACAGCCATGACAGCCCTGTCCACTAGGGCACTTCTATCAGTTCTGATATCACAGTGGAGCCAAACATGGACTGTTAGCATTCTATGCTCCACCCAGTGTTACCTAGCAACAACCAGGCTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,331 SEQUENCE_TARGET=113,46 P3_COMMENT=CL is CCCTGCTCTTATACAAGGTGG P3_COMMENT=CR is GTCAAACCCAGAGGACCTGA = SEQUENCE_ID=19.RHAP131FG7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCATGGATAACAGGGATGGACAACAAGGAAGAGCTACAGAGACATCCAGACAGCCATTATCTGCCTCATACTATCCCAACTGTTACACTTCCTATCCATACCTGCTGCTTTCAAAACCAAGTCTAGCCCCTTCTGAGAATTATTTAGGGGTGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGAGAACAACTTGCAGAAGAAGGCTCTTTCTTACCTTCCACCACATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,258 SEQUENCE_TARGET=171,52 P3_COMMENT=CL is CCAAGTCTAGCCCCTTCTGA P3_COMMENT=CR is CCATGTGGTGGAAGGTAAGAA = SEQUENCE_ID=20.RHAP131FG2.seq SEQUENCE_TEMPLATE=CCGGGTTTTTGTGTATGCTTGGGTGTGGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATTTGTATTTGTGTTATANNNNNNNNNNNNNTTTGCATGTGCGTGTGTGAGTGTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,149 SEQUENCE_TARGET=31,60 SEQUENCE_TARGET=111,13 P3_COMMENT=CL is CCGGGTTTTTGTGTATGCTT P3_COMMENT=CR is GACACTCACACACGCACATG = SEQUENCE_ID=20.RHAP131FG8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCCGTGGTGGTTCTATTTGCAGCCCTAGCATCTTCCCAGAAGAGGCGTTGAAATGAAGGGGCTACTGAGCTGGCTCAGCAGGAAAAGGCACTTAATATACAACCTGGCAATGCAAAATTCAATCCTAGGAACTCACGTGAGAGGTGGAAGGAGAGAACCAACTCCACAAACTAGTCCTCCAGCCTCCACACATGTGCCCCACCCCCAACTCACATATATAACTACGTCATTAACGTTTTTTTAAATTGTCTTAAGTGACAGCTGTGTGTCTTGACACACTCCTGTAATCCTGGCATGAGAAGCAGGAGTTCGAGGCCACAGCAGCTGACTTTGTCCATCTGCAGACCTTTTAACTCTTCAAGCTGAAGGTGTGGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGCACCTTTCCTTGTGGATTTGGATTTGGAGTTTCCACCTGTCTTTAGTTAATACATTCCCATTGACCATTCTTTTTTAACATTGGTTGTACCAGAGACAACGGGTAGACATCATTTCCTGTGCTGCAAATAGGNGGAACTCCTAGAGCTTTATTGNCTCTGGNNTGTGNANAGNNTGNACTNGAGAACACAAAGNATGTCAATTTNTAGAATNTGACAATTTTAGNGTCNAACATGGGTTGTGT SEQUENCE_INCLUDED_REGION=15,666 SEQUENCE_TARGET=391,44 P3_COMMENT=CL is GGCATGAGAAGCAGGAGTTC P3_COMMENT=CR is ATCCACAAGGAAAGGTGCAC = SEQUENCE_ID=23.RHAP131FH2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCTCACTTCTGCTACACAGTTAGCTTCCTTTTCAAAACCTCGTCTTCCTACTTTGGATCCCTTTCTCACCTCCTCTTCTGCTTCCCTTTTTGTTATGGTGGATTGTTCTTGGCTGTCTGTCTGCTTTATTCTTCTTGATCCCCACACCCCTTATGAGTAGCCATGAGATCTCCAGATGTCCGAGGCTTCTGTTGCTCTTTGGAGTTGACTGACACATGCCTGAACCACAGGGTTCACTGATAGCTCTCTGTGGTCCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCATGTATGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,322 SEQUENCE_TARGET=281,46 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=23.RHAP131FH8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCAGCATTTCCCAAGCTGGAGGAGCTANACAGACAGTAAGTAGTTTTTGGCTAATTAATTATCTTCCATAGAAAACAATGTTTCTCACAATTCGTCCTCTTTGTTAATATATTTTCAGAATGCCCCCCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTCAGCCAGCATGCACTGCGTTTCTGAAATGTCTACTAATAGTAAAGTATGGCTATGATAAGTGACTGGTGACTGATGAGAGAGGTTTCCAGAGCTTCTGGACAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=15,285 SEQUENCE_TARGET=144,46 P3_COMMENT=CL is ATATTTTCAGAATGCCCCCC P3_COMMENT=CR is TCCAGAAGCTCTGGAAACCT = SEQUENCE_ID=24.RHAP131FH9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCTCATTCTCCATCAAATGCCACGAACTTCAATGGCTAACCCTTGGGTTCCGTGTCTATGAGGAAGTATACACAATGCCAATGAGCCAGAGATATTCTGTAACCTTCTATTTCACAGTTCCTGTGATCAGCTCCTTAAAGTGGCAGAGGACATAGGAGTTGGCTTTCTCTAGTCATGTACAGTCTTATAAAAATCATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGCAAGAGGAAAAATAGATTTAGTAAAAGCAAAAAAAATTGGTAATGGAGAAACAAAAATAATATTGAATCAAGACCTGAGCATTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=15,337 SEQUENCE_TARGET=214,50 P3_COMMENT=CL is GCTCCTTAAAGTGGCAGAGG P3_COMMENT=CR is TCTCCATTACCAATTTTTTTTGC = SEQUENCE_ID=25.RHAP131FA10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCCAGGAAGATATTTTATACATAAAATATTGAATATGTTTTACCACAATGGGGCATAGCACAATGTAAAGCATTTCACTATCATAATAGAGAGTTTTTGATTCTAGAAATGTGCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACAGAGCCCACAGGGAGAGNNNNNNNNNNNNNNAGAGAGAAATACAAAATGTAATTATCTTTAATTCTAAGAACCCTGCTATGATGGTTAGTTTTTATTGCCAACTTGGATGGGTTTAGAACCAGCCTGGCAACAAAACTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=27,304 SEQUENCE_TARGET=143,42 SEQUENCE_TARGET=206,14 P3_COMMENT=CL is TGGGGCATAGCACAATGTAA P3_COMMENT=CR is CTCTCCCTGTGGGCTCTGT = SEQUENCE_ID=26.RHAP131FA11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCCTACTACAACAAATCAATCTAAAAATAGACCTTTTATTAGCTCACAATTTTAAGGATTTTCTTTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=15,130 SEQUENCE_TARGET=15,57 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=26.RHAP131FA5.seq SEQUENCE_TEMPLATE=CCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCCTCATTGTTGTTTTGATTTCAGGTTTATGCTAACTGAATTTTGTGTTACCTTGACTGGGTCATGAGACCATGCTATTCCTTTGAACCTTTTTCTGACTTTTGTGAGGACACTTTCTGTTGAGCTCAATATAGACTGATGAAAGTAGATTTTCCTCCCTGACAGAAGCCCATCTAACCAGCCTGAGCAAAATGATTAGTCTCCTTTCAGGTGGAAGCCAACTCCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,277 SEQUENCE_TARGET=3,42 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=27.RHAP131FA12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCGACAAAGATGACATGGTTTCTANTGGAGGCAGGGCTGAGATGAGGAGTCACATTCTCTATTGGCAGGCATTTCCCCCTGTCTTGTTATTTTCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNNNNNNNNNNNNNNTGTNNCNNNNNGNGNNNNNCNCNACACAACGNNTGTGCGTGAAAANTGAAGTTTTTTGTGTGTCAGTGAGACAGTGTCTTCNTGGGTGCCCGAGGCAANCNAGGGNATCCACCCGAGCGTTAGCCTCCAGANATTATTAACAANGGCCAAAACATCATNCCTACCTAAGTGAGAAAAAGCAATTTNGANAAAAANCGNGCTCCNAATCCAAAACNTGGGCATGGGCTAGATCCNNAGGTGAAAATNGTAGGCGGTCNAGATTCCNAACAACNAANNANCCGGAAACATGNTGTGTGATAACCANAGGGTGACNNNNGAGTNANNNACACAAAATAANTGCGGNGCGCACAAAGNGCGNNNTCCAGNGGGGAAACCTGNGNGNGCCAACTTNNATAAANNNGNNGGGAANNANCGGGNGNGTTGCGAGGTTGNGNAANNNNNCCNCGGNAGNNTNGTANNNNCNCGNNNANGNGGGGGGAGNGNGNTTGCNNNNNGGGNNNCCTGNNNANNNTTNNNANAGTGNAANGGGGANNNCCAANNNNGNAA SEQUENCE_INCLUDED_REGION=14,687 SEQUENCE_TARGET=141,14 SEQUENCE_TARGET=111,28 P3_COMMENT=CL is CTCTATTGGCAGGCATTTCC P3_COMMENT=CR is AGACACTGTCTCACTGACACACAA = SEQUENCE_ID=29.RHAP131FB5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCTTGGTGCATGCAGCCACATTTCCTCAAGGAGCATAGGGCTTATACAGTTCTTTCAGCACAAGTTTTGGGTCTTTTGTTCCACATTCACATGTCAGACTTGCTAGCCGGTTTTTTGTATATGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATATGTATTTGTGTTATATGTGTGTGTGTGTTTGCAAGTGCGTGGTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTTGTATTCGTGTTATTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTGTGTGTGTGCTATTTCTATNNGTTTTTATNTGTANNNNNNNNNNNNNNNNATNTGCGTGTGTGTGTGCNNNNNNNNNNNNNNNNNNNNNGTGTNNTCCNCNCCNGTATTTCACACNNAANACGTNNANNGNGAGAATTCCTNAAAATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,477 SEQUENCE_TARGET=148,44 SEQUENCE_TARGET=243,35 SEQUENCE_TARGET=301,40 SEQUENCE_TARGET=382,16 SEQUENCE_TARGET=416,18 P3_COMMENT=CL is CATGTCAGACTTGCTAGCCG P3_COMMENT=CR is ACGCACTTGCAAACACACAC = SEQUENCE_ID=30.RHAP131FB12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCGGACAGCTTTCTCCCTAGCTTTTCATTTCTGACAATAGTGCTCTGCCTTGATTCTAGAATGATTTCATGGAGGGAAGTTTGTCCTTTTTAGAGCTATCACAGCTTCACAGTCCTACCCACTTCACTTGTTTCTATGCCAATTCCCTAATACTTGGAGCAGTAATCACACAGCAATACCACAAGGGTGCTTACCCACAGTGCTTTCCTCAGTCAGGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGGTGCTTACCCACAGTGCTTTCCTTAGTCAGGAGCGTGTGTGTGTGTGGGGGGGGGGGGAGGCCAAGATGATAGCCCCTGGGATGGCACAGGTTACTTCCTTGGAGGACCTAACACCAAGACCAATGTGACCATTGCAGCCCACATCTGCACCATCTTTAGAACAACATCCTTTAGGGATACCCTCAAGAAAACACCAGTGATCAGTCTGTGATAGGCAGAGGACAGGTAGGGCNCNTCAAGCCCTTGATCCNCGTGTCNNTGACCACGCCTGGTGATGGGGATCTCNCGTCTTGTTTACCCCATGAGATGGCAGACCCACATGGGGGACGAGCACGNATCNGAAACATGGCAGAGNTGTTCCTGTGTAAANTGGTANCGGCACACTC SEQUENCE_INCLUDED_REGION=14,666 SEQUENCE_TARGET=234,54 P3_COMMENT=CL is GCAATACCACAAGGGTGCTT P3_COMMENT=CR is GGAAAGCACTGTGGGTAAGC = SEQUENCE_ID=31.RHAP131FC4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNCAAATGTGAGCAGATACTCTACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAATGTCATGATAAGGACCACTTTTGTACACTAACAAAAAACTGGATTTAAGCAATATATTTTAAAAACCTCAGCTATATAATAACAAAATAAAAGGGATAATAGAGCTATATGACATCTTACACACATATGCACACCATAGATGTTGTGTAGAGAAAGATGACACTGAGTTCCTGACCCCCAGCTCCTCCCACTGAGTACTATAATCACAGGTGTGTACCAGCAGTCCAGTTTCATTGCNTTAAGAATTAAAGCCAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=24,327 SEQUENCE_TARGET=48,42 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=32.RHAP131FC5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCCCTGTGTTTCATTCTGAGTGCTACAGAGAAGCATTTTTGACAAGTTTAAGCTCTTACTTAAACTTGAGACCAATGATGAGTGAAAGGCATATCCTATCAAATTGCATACATGATATGCAAAATTAAGTCTACATTTTAACATGCCTCGCCTATTGTGGACATATTCTTTTATGAATTGTGTTTAACTACTTACTAAAGGTAGCTCTTTTCCTTTGTGTGTGCATGCACNNNNNNNNNNNNNNNNNNNNNNNNNNAGNGNGACNNNNNNNNNNNNNNNNNNNNNNNNAAAAGAACAGNTTTTNAAAAGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,311 SEQUENCE_TARGET=240,26 SEQUENCE_TARGET=274,24 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=33.RHAP131FC12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNTTGTATTAGAACACTGAATAACTCTTATCTTTACTCTGTAATTTAAAAATTACATTCTTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCATATATGGAAGTCAGAGGACAATTTGTGGGAAGTTCTCTCTCTATACTGTGTGGGTCCTGAGGAATGAATTTATGTCACCAGGCTTGGTGACCAGCACCTTGACTGGATAATCCACCTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=13,232 SEQUENCE_TARGET=75,44 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=33.RHAP131FC6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNCCGCCTCCAAGGACTCCAAGACTGGATGCACACGCTGAGGCAAGACTGCTGAGGCGACACATGGAGGACACATGATGGTTAGAGGGTATATAATAAGGACTCCACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCACTCGGCTTCCTGGTACACTAGTTGTGTCACACTTGCTGGGCTTTGCTGACCTTTGCTTCACTCAGAGAGGCACAGCTGAAGAACGTCTGATGTTCCTGCTGGTCCCTCCTGCTGACTGGAGCCCGGGCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=10,330 SEQUENCE_TARGET=118,44 SEQUENCE_TARGET=163,40 P3_COMMENT=CL is ACATGGAGGACACATGATGG P3_COMMENT=CR is GTGTACCAGGAAGCCGAGTG = SEQUENCE_ID=35.RHAP131FD11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNCAGTGGTTAGCAAGCAACACTGAAGAGACTTGTCAGGTTATATTTATATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGCAACAATGATTAAAGAAGAGGAGGCTGTGAAATTTAAGAGCGAGCAGGGGTGGGTATTGAAGAGATTAGAGGGGGAATGTAATTATGTCTTCATTAACTTTTTCAAATTAAAAAAAAAGAATGTGACTTTAATCATATCTAAATCGGAGCAGACTATCAATCCACATACTCCACTCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=25,317 SEQUENCE_TARGET=77,83 P3_COMMENT=CL is CAACACTGAAGAGACTTGTCAGG P3_COMMENT=CR is ACCCCTGCTCGCTCTTAAAT = SEQUENCE_ID=35.RHAP131FD5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNGNTTAAAATTTCAGTTGTCTTCACTTCACATCCAAGGCTGGATTTCTTGGGATGTGATACAGTGCGGATGGTGCTTGTGGGATCACACAATCTTATGAGACCTGTCTTTCTGTGTTGCCTGTAAACTGTTTCCTAAATANTTTGAGTAGATCTGTTACCTTCTAAATAAGGTTAGATCACTCAAATAACGTATTTGTCTCTAGCTTCTATCTCAGTCACATTTTAATTATTTATACCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGTTTCCATTAACTTTGCCTTNTGANGANGACTCCTTTNTCTTTCANTCTNTTGGGNGGTANCGAAGCTCGNANTCGNNAATNGNNGTCNNNGNTGTTTTCCNGTGTNANNTTNNTNNCCGCNCACNATTCCANNCNNAATANGNNGCGGNAGGATANAAGTGGTANANCNGGGGGNNNNNAAANGNGTTGGGNNANTCAGNNTTANNNGGGGGGNNNNTNNCGNTTNNCAANNNGGGNAANNNNNNNTGNANNNGNNTTAATGNNTNGGGNNANG SEQUENCE_INCLUDED_REGION=11,568 SEQUENCE_TARGET=250,50 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=38.RHAP131FE11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCAATGAGCAACCCTGTCTTAAAACAACTGTGTGTGTTTTTCGGGGGGTCAAGGGGGCTGTTCCTGAGGAATGATTCAAGGTTGATCTCTGGCACCTGTGCTTGTGTGCACACATATGCACAAGTGTATGCACACATACACAACAGAATCTTCCAACACTTTGACCTTGGACTTTCCCTCTTTCAGAAGCAAGAGAAACAGATATCTGTTGTTTAAACCACTTAGTCTACAGTGATTGGTCACTTGGTGTCCTGTGCTATGAGGCGTGTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTGAAATTAGAAACCACCCTTATCACCATGGTTTTCAGAGCCCAGTATCAGTTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,402 SEQUENCE_TARGET=286,70 P3_COMMENT=CL is CTGTGCTATGAGGCGTGTGT P3_COMMENT=CR is ACTGGGCTCTGAAAACCATG = SEQUENCE_ID=38.RHAP131FE5.seq SEQUENCE_TEMPLATE=NNNNNNNNNCANCTCCGAAAAAAANNCCAAAACTAGTTCTCAGCTCTAGATAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCCTTACCTTGATCCTGACGCCCTTGTGTCATTTGTAGGTCAACCAACCACCCATACGTTTCAAGCAATAAAGAATCCAGAGAAAATAATCACNNNNNNNNNNNNNNNNNNGAGTTTTCTGACAGATGCCCAAAGATGCTTTCCAAAGCGTATGTTAGAAAGTGTGAACGAATGAATTCCTCTCTCCCTTCCCGCCCTAAAACACGAACTCACATTCTCCCAGAGATCCACTCCACTCTCCTGTTCCTTCTGTGATAGTTAATTTTGTCTCTTAGCATCACTAGATTTCCAGTCACCTAAGAAACATACCCCTGGGTGTGTGTGTCTTTTGGGGTGATCCTGCAAAAGGTTTAACTGAGGAGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=9,456 SEQUENCE_TARGET=59,32 SEQUENCE_TARGET=190,18 P3_COMMENT=CL is CTTACCTTGATCCTGACGCC P3_COMMENT=CR is GCTTTGGAAAGCATCTTTGG = SEQUENCE_ID=40.RHAP131FF4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCAGTCACACAAAGATAGAGTATAGAATAGAGTTTATTTAGGGCATGGGGAGGAGAGTTGAAAGGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGGAGTAGAGGCTGGTGGTGAGCACGTAGAGAGAAGGGAAGGGGCAGGGGAAGGAGAGAAAGGGAGAGCAGGAACAAGAGAGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,212 SEQUENCE_TARGET=86,58 P3_COMMENT=CL is CATGGGGAGGAGAGTTGAAA P3_COMMENT=CR is CCCCTTCCCTTCTCTCTACG = SEQUENCE_ID=43.RHAP131FG10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCATACACAGAACTAGGGTCTATACACAGTACTAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTACAGACATGAATGCATAGTGTAGGCATAAAAAGAAAGGCTCGATTTCATTCTTTTATCCACACTGTTCACCTGTGTCACCTGTTTTGAGCATGTGAGTCTGTTTTGAGCATGCTAGCCTGTATATAACTAGAGGAACTTAACAAGACTGCACAAGTGAACTCAGGGTAAGGACATATATAAAAGAACATTACAATAGGCAGGGTGAAGTGGGAACAGCAATCCCAGAACACATTCTGGAAATGTTGTAGTTTGAGGAGGAAGAGAGGGAAAGGTCACTCTGGGCATAATTTGGCTTTAACATCAATTTTCCTTCTGGTAGGAGTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,414 SEQUENCE_TARGET=60,46 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=47.RHAP131FH5.seq SEQUENCE_TEMPLATE=ANNTNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGTTNGTGTCTGGGGGGAGGCTNCTCTCCTGTGTGNTGCAGCCANTGGGGTTGGGGAGGAGCANANAGTTTGCNTTNNTGGAGTGAATCAACCAAATTTTGGGAGTTGGAAGTATTTTATTANAAACATNTGGAGATTTTAGAATTTTTTNAAAATGTTGTTATTTAAANCCTTTTCAACGTTAGAAAGTAATAAGTAGGGGTGGGNGGNACGCCCTCNNAGGNNNTTGTTTCAAAANGNNTCNCCCAACTCCCATGANTTTCTTGGNTTTTTTTNNTTNCCGGNAACCNTGNTCTCCTNNAANATCAAAACCCATCAACTTTTGGNGGCCANAATAATCNNTTTTTACTANANTGNNTGNGNGTCCCTTGGTANANTNGNTTNNGGGGGTANCNGANCTCNANNTTCNNTAANTNATGGGNNAAANNGGTTTTTTTNCNTGGGGNNNANTTGTTTTNNTCTNNCCNNNNATTTTTNCNACATTGANANNNNGGNNCCCGGGGCGCNATTNNNGGTGTAANNNCCTGGGGGNNNNNTAANNGNGNGGNNNNNNCCTCANTTTTTANTGGGCGGTNNNCCNCNNNTGCCCNTTTTTTCTGNNTGGGGAAANNTTGTTNNNNNNNNTNG SEQUENCE_INCLUDED_REGION=0,663 SEQUENCE_TARGET=6,29 P3_COMMENT=CL is P3_COMMENT=CR is = SEQUENCE_ID=48.RHAP131FH12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCAGGCATGNGTGAGGGGATGAGAACCCACCTGCAATGTNAAGGAAGTGATGTGATTATTGTTCATGCCCATGTCTCATTCTTCTCCCTTTCCCATGTTGGTCCTGATGGAAATGGAAAAAATAGAGTTGGGGGAAACTTGGAGACCTTATTGTAATATTAGAGACTATGAGGAATCTTAGGATTCTTTTGGGAAAGGCTTGGGCATGTCTTATGATAGATTTCAAGCTAGTAACAACACATCCAAGGATGCTTCTTAAGAAATTTAATTAATAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATTCAGGTCAACATAGTTGCTTGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,348 SEQUENCE_TARGET=297,40 P3_COMMENT=CL is AGGCTTGGGCATGTCTTATG P3_COMMENT=CR is TCCACAAGCAACTATGTTGACC = primer3-2.3.6/test/primer_lib_amb_codes_output0000664000077300007730000004526612231156245020535 0ustar ioanaioanaPRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=1 PRIMER_OPT_SIZE=20 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MAX_SELF_END=8 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=0 PRIMER_MIN_SIZE=18 PRIMER_MAX_SIZE=25 PRIMER_OPT_TM=60.0 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_GC=0.0 PRIMER_MAX_GC=100.0 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_SALT_MONOVALENT=50.0 PRIMER_DNA_CONC=50.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_PRODUCT_SIZE_RANGE=75-500 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib SEQUENCE_ID=test_01 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Should succeed PRIMER_LEFT_EXPLAIN=considered 2424, ok 2424 PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424 PRIMER_INTERNAL_EXPLAIN=considered 3555, GC content failed 92, ok 3463 PRIMER_PAIR_EXPLAIN=considered 94, unacceptable product size 90, ok 4 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.364612 PRIMER_LEFT_0_PENALTY=0.346766 PRIMER_RIGHT_0_PENALTY=0.017846 PRIMER_INTERNAL_0_PENALTY=0.339231 PRIMER_LEFT_0_SEQUENCE=CGACTCTAGAGGATCCCCCT PRIMER_RIGHT_0_SEQUENCE=AGCCTGCCTTTGCAAACTTA PRIMER_INTERNAL_0_SEQUENCE=GGAAGAGAGGGTCATGCAAC PRIMER_LEFT_0=18,20 PRIMER_RIGHT_0=122,20 PRIMER_INTERNAL_0=72,20 PRIMER_LEFT_0_TM=59.653 PRIMER_RIGHT_0_TM=60.018 PRIMER_INTERNAL_0_TM=59.661 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=5.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=105 = PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 SEQUENCE_ID=test_02 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Should still succeed PRIMER_LEFT_EXPLAIN=considered 2424, ok 2424 PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424 PRIMER_INTERNAL_EXPLAIN=considered 3555, GC content failed 92, ok 3463 PRIMER_PAIR_EXPLAIN=considered 94, unacceptable product size 90, ok 4 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.364612 PRIMER_LEFT_0_PENALTY=0.346766 PRIMER_RIGHT_0_PENALTY=0.017846 PRIMER_INTERNAL_0_PENALTY=0.339231 PRIMER_LEFT_0_SEQUENCE=CGACTCTAGAGGATCCCCCT PRIMER_RIGHT_0_SEQUENCE=AGCCTGCCTTTGCAAACTTA PRIMER_INTERNAL_0_SEQUENCE=GGAAGAGAGGGTCATGCAAC PRIMER_LEFT_0=18,20 PRIMER_RIGHT_0=122,20 PRIMER_INTERNAL_0=72,20 PRIMER_LEFT_0_TM=59.653 PRIMER_RIGHT_0_TM=60.018 PRIMER_INTERNAL_0_TM=59.661 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=5.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=105 = SEQUENCE_ID=test_03 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG SEQUENCE_PRIMER=CGACTCTAGAGGATCCCCCT SEQUENCE_PRIMER_REVCOMP=TAGCCTGCCTTTGCAAACTT SEQUENCE_INTERNAL_OLIGO=GGAAGAGAGGGTCATGCAAC PRIMER_PICK_ANYWAY=1 P3_COMMENT=Both primers should have high repeat similarity PRIMER_PAIR_EXPLAIN=considered 1, high mispriming library similarity 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.364612 PRIMER_LEFT_0_PENALTY=0.346766 PRIMER_RIGHT_0_PENALTY=0.017846 PRIMER_INTERNAL_0_PENALTY=0.339231 PRIMER_LEFT_0_PROBLEMS= Similarity to non-template sequence too high; PRIMER_RIGHT_0_PROBLEMS= Similarity to non-template sequence too high; PRIMER_LEFT_0_SEQUENCE=CGACTCTAGAGGATCCCCCT PRIMER_RIGHT_0_SEQUENCE=TAGCCTGCCTTTGCAAACTT PRIMER_INTERNAL_0_SEQUENCE=GGAAGAGAGGGTCATGCAAC PRIMER_LEFT_0=18,20 PRIMER_RIGHT_0=123,20 PRIMER_INTERNAL_0=72,20 PRIMER_LEFT_0_TM=59.653 PRIMER_RIGHT_0_TM=60.018 PRIMER_INTERNAL_0_TM=59.661 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=20.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=20.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=40.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=106 = SEQUENCE_ID=test_04 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG SEQUENCE_PRIMER=CGACTCTAGAGGATCCCCCT SEQUENCE_PRIMER_REVCOMP=TAGCCTGCCTTTGCAAACTT SEQUENCE_INTERNAL_OLIGO=GGAAGAGAGGGTCATGCAAC PRIMER_PICK_ANYWAY=1 P3_COMMENT=Should succeed and both primers should have low repeat similarity PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.364612 PRIMER_LEFT_0_PENALTY=0.346766 PRIMER_RIGHT_0_PENALTY=0.017846 PRIMER_INTERNAL_0_PENALTY=0.339231 PRIMER_LEFT_0_SEQUENCE=CGACTCTAGAGGATCCCCCT PRIMER_RIGHT_0_SEQUENCE=TAGCCTGCCTTTGCAAACTT PRIMER_INTERNAL_0_SEQUENCE=GGAAGAGAGGGTCATGCAAC PRIMER_LEFT_0=18,20 PRIMER_RIGHT_0=123,20 PRIMER_INTERNAL_0=72,20 PRIMER_LEFT_0_TM=59.653 PRIMER_RIGHT_0_TM=60.018 PRIMER_INTERNAL_0_TM=59.661 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=106 = SEQUENCE_ID=test_05 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Both primers should have high repeat similarity, but the explain line for right primers will not indicate this because mispriming is calculated "lazily" -- only if there are good left primers. PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, high repeat similarity 2397, ok 0 PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424 PRIMER_INTERNAL_EXPLAIN=considered 3555, GC content failed 92, ok 3463 PRIMER_PAIR_EXPLAIN=considered 32248, unacceptable product size 32248, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=test_06 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_INTERNAL_MISHYB_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG SEQUENCE_PRIMER=CGACTCTAGAGGATCCCCCT SEQUENCE_PRIMER_REVCOMP=TAGCCTGCCTTTGCAAACTT SEQUENCE_INTERNAL_OLIGO=GGAAGAGAGGGTCATGCAAC PRIMER_PICK_ANYWAY=1 P3_COMMENT=Primers should be ok, but not internal oligo PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.364612 PRIMER_LEFT_0_PENALTY=0.346766 PRIMER_RIGHT_0_PENALTY=0.017846 PRIMER_INTERNAL_0_PENALTY=0.339231 PRIMER_INTERNAL_0_PROBLEMS= Similarity to non-template sequence too high; PRIMER_LEFT_0_SEQUENCE=CGACTCTAGAGGATCCCCCT PRIMER_RIGHT_0_SEQUENCE=TAGCCTGCCTTTGCAAACTT PRIMER_INTERNAL_0_SEQUENCE=GGAAGAGAGGGTCATGCAAC PRIMER_LEFT_0=18,20 PRIMER_RIGHT_0=123,20 PRIMER_INTERNAL_0=72,20 PRIMER_LEFT_0_TM=59.653 PRIMER_RIGHT_0_TM=60.018 PRIMER_INTERNAL_0_TM=59.661 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_INTERNAL_0_LIBRARY_MISHYB=20.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=106 = SEQUENCE_ID=test_07 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_INTERNAL_MISHYB_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG PRIMER_PICK_ANYWAY=1 P3_COMMENT=Primers should be ok, but not internal oligo PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, high repeat similarity 63, ok 2334 PRIMER_RIGHT_EXPLAIN=considered 2424, high end compl 52, high repeat similarity 72, ok 2300 PRIMER_INTERNAL_EXPLAIN=considered 3555, GC content failed 92, high end compl 75, high repeat similarity 3028, ok 360 PRIMER_PAIR_EXPLAIN=considered 5374476, unacceptable product size 2713327, high end compl 966, no internal oligo 2660183, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=test_08 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_INTERNAL_MISHYB_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGATCATCTTCTGATCCAAGACCCGTGCTAGTCTACTGTATTCTACATGTACCCTTCTACAAGCTTTGAATTCCATAATCATCAGAGAATCCAGAAATTATTCATTATCACATAATAACAACACTCAAATATTTAAAGTTTTATGGTTGAATCTTTATGATTGAACCCATTCTATCAGGTGGTTATTTTATATCTAATAGAATTCATTTCAGGGTTACACAAAGGTCCGAATCTATCCATTTTACTGAG P3_COMMENT=Should fail because of high repeat similarity for all internal oligos PRIMER_LEFT_EXPLAIN=considered 1712, high any compl 6, high end compl 24, high repeat similarity 26, ok 1656 PRIMER_RIGHT_EXPLAIN=considered 1712, high any compl 6, high end compl 25, high repeat similarity 28, ok 1653 PRIMER_INTERNAL_EXPLAIN=considered 2665, GC content failed 297, high any compl 15, high end compl 23, high repeat similarity 1970, ok 360 PRIMER_PAIR_EXPLAIN=considered 2737377, unacceptable product size 1340121, no internal oligo 1397256, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=test_09 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_MISPRIMING_LIBRARY=not_for_production_Xs.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Should succeed PRIMER_WARNING=Unrecognized character (X) in mispriming library, entry L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_EXPLAIN=considered 2424, ok 2424 PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424 PRIMER_INTERNAL_EXPLAIN=considered 3555, GC content failed 92, ok 3463 PRIMER_PAIR_EXPLAIN=considered 94, unacceptable product size 90, ok 4 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.364612 PRIMER_LEFT_0_PENALTY=0.346766 PRIMER_RIGHT_0_PENALTY=0.017846 PRIMER_INTERNAL_0_PENALTY=0.339231 PRIMER_LEFT_0_SEQUENCE=CGACTCTAGAGGATCCCCCT PRIMER_RIGHT_0_SEQUENCE=AGCCTGCCTTTGCAAACTTA PRIMER_INTERNAL_0_SEQUENCE=GGAAGAGAGGGTCATGCAAC PRIMER_LEFT_0=18,20 PRIMER_RIGHT_0=122,20 PRIMER_INTERNAL_0=72,20 PRIMER_LEFT_0_TM=59.653 PRIMER_RIGHT_0_TM=60.018 PRIMER_INTERNAL_0_TM=59.661 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_INTERNAL_0_LIBRARY_MISHYB=11.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=5.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=105 = SEQUENCE_ID=test_10 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MISPRIMING_LIBRARY=not_for_production_Xs.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Should fail (Xs are treated as Ns). PRIMER_WARNING=Unrecognized character (X) in mispriming library, entry L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, high repeat similarity 2397, ok 0 PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424 PRIMER_INTERNAL_EXPLAIN=considered 3555, GC content failed 92, ok 3463 PRIMER_PAIR_EXPLAIN=considered 32248, unacceptable product size 32248, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=test_11 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MISPRIMING_LIBRARY= PRIMER_INTERNAL_MISHYB_LIBRARY=not_for_production_Xs.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Should fail (Xs are treated as Ns). PRIMER_WARNING=Unrecognized character (X) in internal oligo mishyb library, entry L1 Human L1 interspersed repetitive sequence - full length copy (for internal oligo) PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, ok 2397 PRIMER_RIGHT_EXPLAIN=considered 2424, high end compl 52, ok 2372 PRIMER_INTERNAL_EXPLAIN=considered 3555, GC content failed 92, high end compl 75, high repeat similarity 3028, ok 360 PRIMER_PAIR_EXPLAIN=considered 5691246, unacceptable product size 2862933, high end compl 994, no internal oligo 2827319, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = primer3-2.3.6/test/th-w-other-tasks_input0000664000077300007730000001513712231156245017327 0ustar ioanaioanaPRIMER_TASK=generic PRIMER_OPT_SIZE=20 PRIMER_MIN_SIZE=19 PRIMER_MAX_SIZE=21 PRIMER_NUM_RETURN=1 PRIMER_MAX_SELF_ANY_TH=47.00 PRIMER_MAX_SELF_END_TH=47.00 PRIMER_MAX_HAIRPIN_TH=47.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.00 PRIMER_PAIR_MAX_COMPL_END_TH=47.00 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=80.00 PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY=../../src/humrep_and_simple.txt SEQUENCE_ID=pick_detection_primers_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_detection_primers_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC = PRIMER_TASK=pick_left_only PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_left_only_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_left_only_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC = PRIMER_TASK=pick_right_only PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_right_only_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_right_only_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC = PRIMER_TASK=pick_hyb_probe_only PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_hyb_probe_only_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_hyb_probe_only_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC = PRIMER_TASK=pick_primer_list PRIMER_NUM_RETURN=10000 PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_primer_list_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 = PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_pick_primer_list_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC = PRIMER_TASK=pick_primer_list PRIMER_PRODUCT_SIZE_RANGE=75-200 P3_FILE_FLAG=1 SEQUENCE_ID=pick_primer_list_and_file_flag_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 = PRIMER_PRODUCT_SIZE_RANGE=75-100 P3_FILE_FLAG=1 SEQUENCE_ID=pick_primer_list_and_file_flag_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC = PRIMER_TASK=pick_primer_list PRIMER_PRODUCT_SIZE_RANGE=75-200 P3_FILE_FLAG=1 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=4 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=4 SEQUENCE_ID=pick_primer_list_and_file_flag_th_3 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 = PRIMER_PRODUCT_SIZE_RANGE=75-100 P3_FILE_FLAG=1 SEQUENCE_ID=pick_primer_list_and_file_flag_th_4 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC = PRIMER_TASK=pick_sequencing_primers PRIMER_PICK_LEFT_PRIMER=0 PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_NUM_RETURN=1 P3_FILE_FLAG=0 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1 PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_sequencing_primers_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 = PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_ID=pick_sequencing_primers_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC = PRIMER_PRODUCT_SIZE_RANGE=75-100 PRIMER_PICK_RIGHT_PRIMER=1 SEQUENCE_ID=pick_sequencing_primers_th_3 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC = PRIMER_TASK=check_primers PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=check_primers_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 SEQUENCE_PRIMER=GACATGACACTGACGATGCA SEQUENCE_PRIMER_REVCOMP=TAGCTATCGATTTGGGTCGG = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=check_primers_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC SEQUENCE_PRIMER=TGACCTGATAGAAACGCCAA SEQUENCE_PRIMER_REVCOMP=TGCTTTGCGTTTTGGAATAG = PRIMER_TASK=pick_discriminative_primers PRIMER_PRODUCT_SIZE_RANGE=75-200 SEQUENCE_ID=pick_disc_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=37,21 PRIMER_PICK_ANYWAY=1 = PRIMER_PRODUCT_SIZE_RANGE=75-100 SEQUENCE_ID=pick_disc_primer_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC SEQUENCE_TARGET=37,21 = PRIMER_TASK=pick_cloning_primers PRIMER_PRODUCT_SIZE_RANGE=200-300 SEQUENCE_ID=pick_disc_th_1 SEQUENCE_TEMPLATE=GTAGTCAGTAGACATGACACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG SEQUENCE_TARGET=2,100 = PRIMER_PRODUCT_SIZE_RANGE=200-300 SEQUENCE_ID=pick_disc_primer_th_2 SEQUENCE_TEMPLATE=GANAATGCAGTAATGAGTTGGCAAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACGTTGTCAAAGTCTATTCCAAAACGCAAAGCACGCTCCCGATC SEQUENCE_TARGET=2,120 = primer3-2.3.6/test/dpal_output0000664000077300007730000006667312231156245015342 0ustar ioanaioana|AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| l score=14.00 len=17 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| e score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| g score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| L score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| l score=1.00 len=1 |16,17| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| e score=1.00 len=1 |16,0| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| g score=1.00 len=1 |16,0| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| L score=1.00 len=1 |16,17| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| l score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| e score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| g score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| L score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| l score=2.00 len=2 |6,16|7,17| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| e score=-1.00 len=1 |20,0| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| g score=1.00 len=1 |0,18| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| L score=0.00 len=0 | |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| l score=1.00 len=1 |10,9| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| e score=-1.00 len=1 |19,0| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| g score=-1.00 len=1 |19,0| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| L score=0.00 len=0 | |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| l score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| e score=4.00 len=4 |16,0|17,1|18,2|19,3| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| g score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| L score=4.00 len=4 |16,13|17,14|18,15|19,16| |AAACGATCGATTTT| |AAACGATCGACCCC| l score=10.00 len=10 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9| |AAACGATCGATTTT| |AAACGATCGACCCC| e score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAACGATCGATTTT| |AAACGATCGACCCC| g score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAACGATCGATTTT| |AAACGATCGACCCC| L score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| l score=1.00 len=1 |15,13| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| e score=-1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| g score=-1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| L score=1.00 len=1 |15,13| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| l score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| e score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| g score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| L score=1.00 len=1 |15,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| l score=0.00 len=0 | |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| e score=-1.00 len=1 |14,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| g score=-1.00 len=1 |14,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| l score=1.00 len=1 |0,8| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |0,15| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| g score=1.00 len=1 |0,15| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |0,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 | |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |7,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 | |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |7,15| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| g score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 | |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |14,7| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| L score=1.00 len=1 |14,7| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |0,7| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| L score=0.00 len=0 | |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| l score=14.00 len=17 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| e score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| g score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| L score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| l score=1.00 len=1 |16,17| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| e score=1.00 len=1 |16,0| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| g score=1.00 len=1 |16,0| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| L score=1.00 len=1 |16,17| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| l score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| e score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| g score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| L score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| l score=2.00 len=2 |6,16|7,17| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| e score=-1.00 len=1 |20,0| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| g score=1.00 len=1 |0,18| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| L score=0.00 len=0 | |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| l score=1.00 len=1 |10,9| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| e score=-1.00 len=1 |19,0| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| g score=-1.00 len=1 |19,0| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| L score=0.00 len=0 | |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| l score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| e score=4.00 len=4 |16,0|17,1|18,2|19,3| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| g score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| L score=4.00 len=4 |16,13|17,14|18,15|19,16| |AAACGATCGATTTT| |AAACGATCGACCCC| l score=10.00 len=10 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9| |AAACGATCGATTTT| |AAACGATCGACCCC| e score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAACGATCGATTTT| |AAACGATCGACCCC| g score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAACGATCGATTTT| |AAACGATCGACCCC| L score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| l score=1.00 len=1 |15,13| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| e score=-1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| g score=-1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| L score=1.00 len=1 |15,13| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| l score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| e score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| g score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| L score=1.00 len=1 |15,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| l score=0.00 len=0 | |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| e score=-1.00 len=1 |14,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| g score=-1.00 len=1 |14,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| l score=1.00 len=1 |0,8| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |0,15| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| g score=1.00 len=1 |0,15| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |0,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 | |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |7,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 | |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |7,15| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| g score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 | |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |14,7| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| L score=1.00 len=1 |14,7| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |0,7| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| L score=0.00 len=0 | |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| l score=14.00 len=17 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| e score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| g score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| L score=12.00 len=19 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| l score=1.00 len=1 |16,17| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| e score=1.00 len=1 |16,0| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| g score=1.00 len=1 |16,0| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| L score=1.00 len=1 |16,17| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| l score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| e score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| g score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| L score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| l score=2.00 len=2 |6,16|7,17| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| e score=-1.00 len=1 |20,0| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| g score=1.00 len=1 |0,18| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| L score=0.00 len=0 | |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| l score=1.00 len=1 |10,9| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| e score=-1.00 len=1 |19,0| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| g score=-1.00 len=1 |19,0| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| L score=0.00 len=0 | |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| l score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| e score=4.00 len=4 |16,0|17,1|18,2|19,3| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| g score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| L score=4.00 len=4 |16,13|17,14|18,15|19,16| |AAACGATCGATTTT| |AAACGATCGACCCC| l score=10.00 len=10 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9| |AAACGATCGATTTT| |AAACGATCGACCCC| e score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAACGATCGATTTT| |AAACGATCGACCCC| g score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAACGATCGATTTT| |AAACGATCGACCCC| L score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| l score=1.00 len=1 |15,13| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| e score=-1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| g score=-1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| L score=1.00 len=1 |15,13| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| l score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| e score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| g score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| L score=1.00 len=1 |15,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| l score=0.00 len=0 | |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| e score=-1.00 len=1 |14,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| g score=-1.00 len=1 |14,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| l score=1.00 len=1 |0,8| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |0,15| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| g score=1.00 len=1 |0,15| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |0,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 | |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |7,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 | |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |7,15| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| g score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 | |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |14,7| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| L score=1.00 len=1 |14,7| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |0,7| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| L score=0.00 len=0 | |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| l score=9.00 len=9 |2,0|3,1|4,2|5,3|6,4|7,5|8,6|9,7|10,8| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| e score=5.00 len=19 |3,0|4,1|5,2|6,3|7,4|8,5|9,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| g score=5.00 len=19 |3,0|4,1|5,2|6,3|7,4|8,5|9,6|10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAAACCCGCTTTGCTAGCTACG| |AACCCGCTTGCTAGCAAAAAAAA| L score=6.00 len=12 |10,7|11,8|12,9|13,10|14,11|15,12|16,13|17,14|18,15|19,16|20,17|21,18| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| l score=1.00 len=1 |16,17| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| e score=1.00 len=1 |16,0| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| g score=1.00 len=1 |16,0| |AAACACACACACACACT| |TGGGGGTGTTGGGGTTGTGG| L score=1.00 len=1 |16,17| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| l score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| e score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| g score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |AAACGATCGTAGCGTTTCAGTCT| |AGTCTCTCTCTCTCTCTCTC| L score=5.00 len=5 |18,0|19,1|20,2|21,3|22,4| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| l score=2.00 len=2 |6,16|7,17| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| e score=-1.00 len=1 |20,0| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| g score=1.00 len=1 |0,18| |ACGCCGTTGCGCGCTGCTGCG| |TTTTTTTTTTTTTTTTTTA| L score=0.00 len=0 | |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| l score=1.00 len=1 |10,9| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| e score=-1.00 len=1 |19,0| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| g score=-1.00 len=1 |19,0| |TTTTTTTTTTATTTTTTTTT| |GGGGGGGGGAGGGGGGGGG| L score=0.00 len=0 | |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| l score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| e score=4.00 len=4 |16,0|17,1|18,2|19,3| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| g score=6.00 len=6 |0,13|1,14|2,15|3,16|4,17|5,18| |AACCGGGTCTTTTTTTAACC| |AACCTTAAAAAAAAACCGG| L score=4.00 len=4 |16,13|17,14|18,15|19,16| |AAACGATCGATTTT| |AAACGATCGACCCC| l score=10.00 len=10 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9| |AAACGATCGATTTT| |AAACGATCGACCCC| e score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAACGATCGATTTT| |AAACGATCGACCCC| g score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAACGATCGATTTT| |AAACGATCGACCCC| L score=6.00 len=14 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,10|11,11|12,12|13,13| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| l score=1.00 len=1 |15,13| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| e score=-1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| g score=-1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |CCCCCCCCCCCCCT| L score=1.00 len=1 |15,13| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| l score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| e score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| g score=1.00 len=1 |15,0| |AAAAAAAAAAAAAAAT| |TCCCCCCCCCCCCC| L score=1.00 len=1 |15,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| l score=0.00 len=0 | |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| e score=-1.00 len=1 |14,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| g score=-1.00 len=1 |14,0| |ACACACACACACACA| |TGTGTTGGTGTTGTTGTGTG| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| l score=1.00 len=1 |0,8| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGTGGGGGGGGG| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |0,15| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| g score=1.00 len=1 |0,15| |TAAAAAAAAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 | |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |0,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 | |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| l score=1.00 len=1 |7,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| e score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| g score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |TGGGGGGGGGGGGGGG| L score=0.00 len=0 | |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| l score=1.00 len=1 |7,15| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| e score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| g score=-1.00 len=1 |14,0| |AAAAAAATAAAAAAA| |GGGGGGGGGGGGGGGT| L score=0.00 len=0 | |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |14,7| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0| |AAAAAAAAAAAAAAT| |GGGGGGGTGGGGGGGG| L score=1.00 len=1 |14,7| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| l score=1.00 len=1 |0,7| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| e score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| g score=-1.00 len=1 |14,0| |TAAAAAAAAAAAAAA| |GGGGGGGTGGGGGGGG| L score=0.00 len=0 | || || l score=0.00 len=0 | || || g score=0.00 len=0 | || || e score=0.00 len=0 | || || L score=0.00 len=0 | |A| || l score=0.00 len=0 | |A| || g score=0.00 len=0 | |A| || e score=0.00 len=0 | |A| || L score=0.00 len=0 | || |A| l score=0.00 len=0 | || |A| g score=0.00 len=0 | || |A| e score=0.00 len=0 | || |A| L score=0.00 len=0 | |AAGATCAGCTAGCTAGCTAGC| |TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT| l score=21.00 len=21 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25| |AAGATCAGCTAGCTAGCTAGC| |TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT| g score=21.00 len=21 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25| |AAGATCAGCTAGCTAGCTAGC| |TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT| e score=21.00 len=21 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25| |AAGATCAGCTAGCTAGCTAGC| |TTTTTAAGATCAGCTAGCTAGCTAGCTTTTTTTT| L score=21.00 len=21 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25| |GATCAGCTAGCTTTTT| |TTTTTTGATCAGCTAGCTTTTT| l score=16.00 len=16 |0,6|1,7|2,8|3,9|4,10|5,11|6,12|7,13|8,14|9,15|10,16|11,17|12,18|13,19|14,20|15,21| |GATCAGCTAGCTTTTT| |TTTTTTGATCAGCTAGCTTTTT| g score=16.00 len=16 |0,6|1,7|2,8|3,9|4,10|5,11|6,12|7,13|8,14|9,15|10,16|11,17|12,18|13,19|14,20|15,21| |GATCAGCTAGCTTTTT| |TTTTTTGATCAGCTAGCTTTTT| e score=16.00 len=16 |0,6|1,7|2,8|3,9|4,10|5,11|6,12|7,13|8,14|9,15|10,16|11,17|12,18|13,19|14,20|15,21| |GATCAGCTAGCTTTTT| |TTTTTTGATCAGCTAGCTTTTT| L score=16.00 len=16 |0,6|1,7|2,8|3,9|4,10|5,11|6,12|7,13|8,14|9,15|10,16|11,17|12,18|13,19|14,20|15,21| |AAGATCAGCTAGCTAGCTAGCTTTTT| |TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT| l score=24.00 len=24 |2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|21,26|22,27|23,28|24,29|25,30| |AAGATCAGCTAGCTAGCTAGCTTTTT| |TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT| g score=22.00 len=26 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|21,26|22,27|23,28|24,29|25,30| |AAGATCAGCTAGCTAGCTAGCTTTTT| |TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT| e score=22.00 len=26 |0,5|1,6|2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|21,26|22,27|23,28|24,29|25,30| |AAGATCAGCTAGCTAGCTAGCTTTTT| |TTTTTCCGATCAGCTAGCTAGCTAGCTTTTTTT| L score=24.00 len=24 |2,7|3,8|4,9|5,10|6,11|7,12|8,13|9,14|10,15|11,16|12,17|13,18|14,19|15,20|16,21|17,22|18,23|19,24|20,25|21,26|22,27|23,28|24,29|25,30| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=16.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=16.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=16.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=16.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=11.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=11.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=11.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=11.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=12.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=12.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=12.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=12.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=8.00 len=18 |0,2|1,3|2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=8.00 len=18 |0,2|1,3|2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,7|8,8|9,9|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| l score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| g score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,8|8,9|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| e score=10.00 len=18 |0,0|1,1|2,2|3,3|4,4|5,5|6,6|7,8|8,9|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| |CGATATATTTGGTCTATA| |CGATATATTTCCGGTCTATA| L score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19| primer3-2.3.6/test/primer_tm_lc_masking_output0000664000077300007730000002423512231156245020573 0ustar ioanaioanaPRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=0 PRIMER_LOWERCASE_MASKING=0 PRIMER_NUM_RETURN=1 PRIMER_TASK=pick_left_only PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_SIZE=10 PRIMER_MAX_SIZE=10 PRIMER_OPT_SIZE=10 PRIMER_MIN_TM=0 PRIMER_MAX_TM=70 P3_COMMENT=nomasking_Breslauer_Schildkraut SEQUENCE_ID=000 SEQUENCE_TEMPLATE=TCCTTGTGAT PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=44.893723 PRIMER_LEFT_0_SEQUENCE=TCCTTGTGAT PRIMER_LEFT_0=0,10 PRIMER_LEFT_0_TM=15.106 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.3000 = PRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=1 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_Breslauer_SantaLucia SEQUENCE_ID=001 SEQUENCE_TEMPLATE=TCCTTGTGAT PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=37.127461 PRIMER_LEFT_0_SEQUENCE=TCCTTGTGAT PRIMER_LEFT_0=0,10 PRIMER_LEFT_0_TM=22.873 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.3000 = PRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=2 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_Breslauer_Owczarzy SEQUENCE_ID=002 SEQUENCE_TEMPLATE=TCCTTGTGAT PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=37.175756 PRIMER_LEFT_0_SEQUENCE=TCCTTGTGAT PRIMER_LEFT_0=0,10 PRIMER_LEFT_0_TM=22.824 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.3000 = PRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=0 PRIMER_LOWERCASE_MASKING=1 P3_COMMENT=masking_Breslauer_Schildkraut P3_COMMENT=Should fail SEQUENCE_ID=100 SEQUENCE_TEMPLATE=TCCTTGTGAt PRIMER_LEFT_EXPLAIN=considered 1, lowercase masking of 3' end 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = PRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=1 PRIMER_LOWERCASE_MASKING=1 SEQUENCE_ID=101 P3_COMMENT=masking_Breslauer_SantaLucia P3_COMMENT=Should succeed SEQUENCE_TEMPLATE=TCCTTGTGaT PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=37.127461 PRIMER_LEFT_0_SEQUENCE=TCCTTGTGaT PRIMER_LEFT_0=0,10 PRIMER_LEFT_0_TM=22.873 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.3000 = PRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=2 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=masking_Breslauer_Owczarzy P3_COMMENT=Should succeed SEQUENCE_ID=102 SEQUENCE_TEMPLATE=tccttgtgat PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=37.175756 PRIMER_LEFT_0_SEQUENCE=tccttgtgat PRIMER_LEFT_0=0,10 PRIMER_LEFT_0_TM=22.824 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.3000 = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=0 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_SantaLucia_Schildkraut SEQUENCE_ID=010 SEQUENCE_TEMPLATE=TCCTTGTGAT PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=50.265050 PRIMER_LEFT_0_SEQUENCE=TCCTTGTGAT PRIMER_LEFT_0=0,10 PRIMER_LEFT_0_TM=9.735 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=3.0600 = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_SantaLucia_SantaLucia SEQUENCE_ID=011 SEQUENCE_TEMPLATE=TCCTTGTGAG PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=39.057206 PRIMER_LEFT_0_SEQUENCE=TCCTTGTGAG PRIMER_LEFT_0=0,10 PRIMER_LEFT_0_TM=20.943 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=3.5100 = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=2 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_SantaLucia_Owczarzy SEQUENCE_ID=012 SEQUENCE_TEMPLATE=GCCTTGTGAG PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=33.922737 PRIMER_LEFT_0_SEQUENCE=GCCTTGTGAG PRIMER_LEFT_0=0,10 PRIMER_LEFT_0_TM=26.077 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=3.5100 = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=0 PRIMER_LOWERCASE_MASKING=1 P3_COMMENT=masking_SantaLucia_Schildkraut P3_COMMENT=Should fail SEQUENCE_ID=110 SEQUENCE_TEMPLATE=TCCTTGTGAt PRIMER_LEFT_EXPLAIN=considered 1, lowercase masking of 3' end 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_LOWERCASE_MASKING=1 P3_COMMENT=masking_SantaLucia_SantaLucia P3_COMMENT=Should succeed SEQUENCE_ID=111 SEQUENCE_TEMPLATE=TCCTTGTGaT PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=41.601530 PRIMER_LEFT_0_SEQUENCE=TCCTTGTGaT PRIMER_LEFT_0=0,10 PRIMER_LEFT_0_TM=18.398 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=3.0600 = PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=2 PRIMER_LOWERCASE_MASKING=0 P3_COMMENT=nomasking_SantaLucia_Owczarzy P3_COMMENT=Should succeed SEQUENCE_ID=112 SEQUENCE_TEMPLATE=tccttgtgat PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=42.080319 PRIMER_LEFT_0_SEQUENCE=tccttgtgat PRIMER_LEFT_0=0,10 PRIMER_LEFT_0_TM=17.920 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=3.0600 = SEQUENCE_ID=masking_001 PRIMER_LOWERCASE_MASKING=1 PRIMER_PRODUCT_SIZE_RANGE=40-300 PRIMER_TASK=pick_pcr_primers PRIMER_MAX_SIZE=20 PRIMER_MIN_SIZE=20 PRIMER_OPT_SIZE=20 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAT PRIMER_LEFT_EXPLAIN=considered 31, too many Ns 30, ok 1 PRIMER_RIGHT_EXPLAIN=considered 31, too many Ns 30, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=27.371182 PRIMER_LEFT_0_PENALTY=13.856830 PRIMER_RIGHT_0_PENALTY=13.514352 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGACA PRIMER_RIGHT_0_SEQUENCE=ATTAGTACCCAGGTGTGATT PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=69,20 PRIMER_LEFT_0_TM=46.143 PRIMER_RIGHT_0_TM=46.486 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=3.5800 PRIMER_RIGHT_0_END_STABILITY=2.5700 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=70 = SEQUENCE_ID=masking_002 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNaATCACACCTGGGTACTAAT PRIMER_LEFT_EXPLAIN=considered 31, too many Ns 30, ok 1 PRIMER_RIGHT_EXPLAIN=considered 31, too many Ns 30, lowercase masking of 3' end 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=masking_003 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAt PRIMER_LEFT_EXPLAIN=considered 31, too many Ns 30, ok 1 PRIMER_RIGHT_EXPLAIN=considered 31, too many Ns 30, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=27.371182 PRIMER_LEFT_0_PENALTY=13.856830 PRIMER_RIGHT_0_PENALTY=13.514352 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGACA PRIMER_RIGHT_0_SEQUENCE=aTTAGTACCCAGGTGTGATT PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=69,20 PRIMER_LEFT_0_TM=46.143 PRIMER_RIGHT_0_TM=46.486 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=3.5800 PRIMER_RIGHT_0_END_STABILITY=2.5700 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=70 = SEQUENCE_ID=masking_004 SEQUENCE_TEMPLATE=gCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAT PRIMER_LEFT_EXPLAIN=considered 31, too many Ns 30, ok 1 PRIMER_RIGHT_EXPLAIN=considered 31, too many Ns 30, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=27.371182 PRIMER_LEFT_0_PENALTY=13.856830 PRIMER_RIGHT_0_PENALTY=13.514352 PRIMER_LEFT_0_SEQUENCE=gCCTTCATTAGAAAGTGACA PRIMER_RIGHT_0_SEQUENCE=ATTAGTACCCAGGTGTGATT PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=69,20 PRIMER_LEFT_0_TM=46.143 PRIMER_RIGHT_0_TM=46.486 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=3.5800 PRIMER_RIGHT_0_END_STABILITY=2.5700 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=70 = SEQUENCE_ID=masking_005 SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACaNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAT PRIMER_LEFT_EXPLAIN=considered 31, too many Ns 30, lowercase masking of 3' end 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 31, too many Ns 30, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = primer3-2.3.6/test/primer_task_output0000664000077300007730000003363412231156245016731 0ustar ioanaioanaPRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 P3_FILE_FLAG=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_TASK=pick_pcr_primers SEQUENCE_ID=pcr_primers SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, ok 3 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 3, ok 3 PRIMER_LEFT_NUM_RETURNED=3 PRIMER_RIGHT_NUM_RETURNED=3 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=3 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 PRIMER_PAIR_1_PENALTY=4.086374 PRIMER_LEFT_1_PENALTY=1.771733 PRIMER_RIGHT_1_PENALTY=2.314641 PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_1=1,20 PRIMER_RIGHT_1=160,18 PRIMER_LEFT_1_TM=58.228 PRIMER_RIGHT_1_TM=59.685 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=10.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=12.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=160 PRIMER_PAIR_2_PENALTY=5.046339 PRIMER_LEFT_2_PENALTY=2.731698 PRIMER_RIGHT_2_PENALTY=2.314641 PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=160,18 PRIMER_LEFT_2_TM=57.268 PRIMER_RIGHT_2_TM=59.685 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY=12.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=12.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=161 = SEQUENCE_ID=pcr_primers_and_io PRIMER_TASK=pick_pcr_primers_and_hyb_PROBE SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG PRIMER_LEFT_EXPLAIN=considered 281, too many Ns 210, low tm 30, high tm 29, ok 12 PRIMER_RIGHT_EXPLAIN=considered 284, too many Ns 210, low tm 7, high tm 44, ok 23 PRIMER_INTERNAL_EXPLAIN=considered 393, too many Ns 280, low tm 30, high tm 46, long poly-x seq 3, ok 34 PRIMER_PAIR_EXPLAIN=considered 5, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.334195 PRIMER_LEFT_0_PENALTY=2.101896 PRIMER_RIGHT_0_PENALTY=0.232299 PRIMER_INTERNAL_0_PENALTY=4.798289 PRIMER_LEFT_0_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_0_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_INTERNAL_0_SEQUENCE=CTTAGCGTGCGTGGGGTC PRIMER_LEFT_0=9,22 PRIMER_RIGHT_0=247,20 PRIMER_INTERNAL_0=141,18 PRIMER_LEFT_0_TM=59.898 PRIMER_RIGHT_0_TM=60.232 PRIMER_INTERNAL_0_TM=62.798 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_INTERNAL_0_GC_PERCENT=66.667 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_INTERNAL_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.4000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=239 PRIMER_PAIR_1_PENALTY=2.773493 PRIMER_LEFT_1_PENALTY=2.101896 PRIMER_RIGHT_1_PENALTY=0.671597 PRIMER_INTERNAL_1_PENALTY=4.798289 PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT PRIMER_INTERNAL_1_SEQUENCE=CTTAGCGTGCGTGGGGTC PRIMER_LEFT_1=9,22 PRIMER_RIGHT_1=246,20 PRIMER_INTERNAL_1=141,18 PRIMER_LEFT_1_TM=59.898 PRIMER_RIGHT_1_TM=59.328 PRIMER_INTERNAL_1_TM=62.798 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_INTERNAL_1_GC_PERCENT=66.667 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=238 PRIMER_PAIR_2_PENALTY=2.846821 PRIMER_LEFT_2_PENALTY=2.101896 PRIMER_RIGHT_2_PENALTY=0.744925 PRIMER_INTERNAL_2_PENALTY=4.798289 PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA PRIMER_INTERNAL_2_SEQUENCE=CTTAGCGTGCGTGGGGTC PRIMER_LEFT_2=9,22 PRIMER_RIGHT_2=248,20 PRIMER_INTERNAL_2=141,18 PRIMER_LEFT_2_TM=59.898 PRIMER_RIGHT_2_TM=60.745 PRIMER_INTERNAL_2_TM=62.798 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_INTERNAL_2_GC_PERCENT=66.667 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=240 PRIMER_PAIR_3_PENALTY=3.098430 PRIMER_LEFT_3_PENALTY=2.866131 PRIMER_RIGHT_3_PENALTY=0.232299 PRIMER_INTERNAL_3_PENALTY=4.798289 PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_INTERNAL_3_SEQUENCE=CTTAGCGTGCGTGGGGTC PRIMER_LEFT_3=11,21 PRIMER_RIGHT_3=247,20 PRIMER_INTERNAL_3=141,18 PRIMER_LEFT_3_TM=58.134 PRIMER_RIGHT_3_TM=60.232 PRIMER_INTERNAL_3_TM=62.798 PRIMER_LEFT_3_GC_PERCENT=38.095 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_INTERNAL_3_GC_PERCENT=66.667 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=237 PRIMER_PAIR_4_PENALTY=3.235559 PRIMER_LEFT_4_PENALTY=3.003259 PRIMER_RIGHT_4_PENALTY=0.232299 PRIMER_INTERNAL_4_PENALTY=4.798289 PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC PRIMER_INTERNAL_4_SEQUENCE=CTTAGCGTGCGTGGGGTC PRIMER_LEFT_4=10,22 PRIMER_RIGHT_4=247,20 PRIMER_INTERNAL_4=141,18 PRIMER_LEFT_4_TM=58.997 PRIMER_RIGHT_4_TM=60.232 PRIMER_INTERNAL_4_TM=62.798 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_GC_PERCENT=66.667 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_INTERNAL_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=238 = SEQUENCE_ID=left_only PRIMER_TASK=pick_left_only SEQUENCE_TARGET=100,20 SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC PRIMER_LEFT_EXPLAIN=considered 1121, too many Ns 38, overlap target 23, low tm 42, high tm 866, ok 152 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.040772 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_0=72,20 PRIMER_LEFT_0_TM=59.959 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_LEFT_1_PENALTY=0.271680 PRIMER_LEFT_1_SEQUENCE=CTGCAGGTTTAGAAGGGGTG PRIMER_LEFT_1=64,20 PRIMER_LEFT_1_TM=59.728 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_LEFT_2_PENALTY=0.271680 PRIMER_LEFT_2_SEQUENCE=CACCCTGCAGGTTTAGAAGG PRIMER_LEFT_2=60,20 PRIMER_LEFT_2_TM=59.728 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_LEFT_3_PENALTY=0.312496 PRIMER_LEFT_3_SEQUENCE=AGCACACCCTGCAGGTTTAG PRIMER_LEFT_3=56,20 PRIMER_LEFT_3_TM=60.312 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=6.3000 PRIMER_LEFT_4_PENALTY=0.407941 PRIMER_LEFT_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT PRIMER_LEFT_4=65,20 PRIMER_LEFT_4_TM=59.592 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.6000 = SEQUENCE_ID=right_only PRIMER_TASK=PICK_right_only SEQUENCE_TARGET=90,10 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC PRIMER_RIGHT_EXPLAIN=considered 623, too many Ns 27, overlap target 36, GC content failed 5, low tm 230, high tm 145, high end compl 6, ok 174 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_RIGHT_0_PENALTY=0.291669 PRIMER_RIGHT_0_SEQUENCE=GTGCTTTGCGTTTTGGAAGT PRIMER_RIGHT_0=125,20 PRIMER_RIGHT_0_TM=60.292 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_RIGHT_1_PENALTY=0.727969 PRIMER_RIGHT_1_SEQUENCE=TTGGCGTTTCTATCAGGTCA PRIMER_RIGHT_1=75,20 PRIMER_RIGHT_1_TM=59.272 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_RIGHT_2_PENALTY=0.782051 PRIMER_RIGHT_2_SEQUENCE=TGCTTTGCGTTTTGGAAGTT PRIMER_RIGHT_2=124,20 PRIMER_RIGHT_2_TM=60.782 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_RIGHT_3_PENALTY=1.125062 PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA PRIMER_RIGHT_3=76,21 PRIMER_RIGHT_3_TM=60.125 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_RIGHT_4_PENALTY=1.255675 PRIMER_RIGHT_4_SEQUENCE=TGAGTTGGCGTTTCTATCAGG PRIMER_RIGHT_4=79,21 PRIMER_RIGHT_4_TM=60.256 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_RIGHT_4_END_STABILITY=8.2000 = SEQUENCE_ID=hyb_probe_only PRIMER_TASK=pick_HYB_probe_ONly SEQUENCE_INTERNAL_EXCLUDED_REGION=90,20 SEQUENCE_TEMPLATE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC PRIMER_INTERNAL_EXPLAIN=considered 1040, too many Ns 27, overlap excluded region 33, low tm 44, high tm 804, ok 132 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_INTERNAL_0_PENALTY=0.271680 PRIMER_INTERNAL_0_SEQUENCE=CTGCAGGTTTAGAAGGGGTG PRIMER_INTERNAL_0=64,20 PRIMER_INTERNAL_0_TM=59.728 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_END=0.00 PRIMER_INTERNAL_1_PENALTY=0.271680 PRIMER_INTERNAL_1_SEQUENCE=CACCCTGCAGGTTTAGAAGG PRIMER_INTERNAL_1=60,20 PRIMER_INTERNAL_1_TM=59.728 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_INTERNAL_1_SELF_ANY=8.00 PRIMER_INTERNAL_1_SELF_END=0.00 PRIMER_INTERNAL_2_PENALTY=0.312496 PRIMER_INTERNAL_2_SEQUENCE=AGCACACCCTGCAGGTTTAG PRIMER_INTERNAL_2=56,20 PRIMER_INTERNAL_2_TM=60.312 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_INTERNAL_2_SELF_ANY=8.00 PRIMER_INTERNAL_2_SELF_END=6.00 PRIMER_INTERNAL_3_PENALTY=0.375090 PRIMER_INTERNAL_3_SEQUENCE=CATCTGCACCCTGTAAACCC PRIMER_INTERNAL_3=1,20 PRIMER_INTERNAL_3_TM=60.375 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_INTERNAL_3_SELF_END=0.00 PRIMER_INTERNAL_4_PENALTY=0.407941 PRIMER_INTERNAL_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT PRIMER_INTERNAL_4=65,20 PRIMER_INTERNAL_4_TM=59.592 PRIMER_INTERNAL_4_GC_PERCENT=50.000 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_INTERNAL_4_SELF_END=0.00 = SEQUENCE_ID=left_only PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.040772 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_0=0,20 PRIMER_LEFT_0_TM=59.959 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 = SEQUENCE_ID=right_only PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.040772 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_0=0,20 PRIMER_LEFT_0_TM=59.959 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 = SEQUENCE_ID=hyb_probe_only PRIMER_INTERNAL_MIN_SIZE=20 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_TASK=pick_left_only SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 PRIMER_LEFT_0_PENALTY=0.040772 PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG PRIMER_LEFT_0=0,20 PRIMER_LEFT_0_TM=59.959 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 = primer3-2.3.6/test/primer_internal_input0000664000077300007730000002426012231156245017375 0ustar ioanaioanaSEQUENCE_ID=boundary_test_00 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_INTERNAL_MIN_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_INTERNAL_MAX_SELF_ANY=100 PRIMER_INTERNAL_MAX_SELF_END=100 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_INTERNAL_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-100 PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_02 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_05 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNCACGTCATCTCATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_MIN_SIZE=24 PRIMER_MAX_SIZE=24 PRIMER_OPT_SIZE=24 PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNCACGTCATCTNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_11_2 PRIMER_MAX_NS_ACCEPTED=2 PRIMER_INTERNAL_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_12 PRIMER_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNCACGTCATCNNATCAAATTTGATTGNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PICK_INTERNAL_OLIGO=1 = SEQUENCE_ID=boundary_test_13 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTTTTTTTTTTTTTTTTTTTTNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_14 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=.0001 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAACAAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_15 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=20 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_16 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_PRODUCT_SIZE_RANGE=50-200 PRIMER_INTERNAL_MAX_NS_ACCEPTED=5 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_17 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=20 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNTTTTTTTTTTTTTTTTTTTTTTTTGNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_18 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INTERNAL_EXCLUDED_REGION=10,50 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNACCGCTATTGGGCCATTTGCCCATTGCATGNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_19 PRIMER_NUM_RETURN=7 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 SEQUENCE_INTERNAL_EXCLUDED_REGION=10,50 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTTGCCCGTATCCCCTGGATNNNCCGTTAGGTGCTAGCCGTGTAGATTNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_20 PRIMER_NUM_RETURN=5 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=100-300 SEQUENCE_INTERNAL_EXCLUDED_REGION=5,50 SEQUENCE_INTERNAL_EXCLUDED_REGION=60,15 SEQUENCE_INCLUDED_REGION=2,250 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNAAGCTAAGCTGTCGATGCGGTGGAGACGATACGGTAAGCTAGCTAACTGGTCAAAGCTAGCGGTAGCTTAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTCACTGTCATTTCCAAACAATTCATCTCTCTCTCATATATATATATATATATATATATATAT = SEQUENCE_ID=boundary_test_21 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_INTERNAL_MIN_TM=55.7 PRIMER_INTERNAL_MAX_TM=55.9 PRIMER_INTERNAL_OPT_TM=55.8 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_22 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=80-80 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_INTERNAL_MIN_SIZE=25 PRIMER_INTERNAL_MAX_SIZE=25 PRIMER_INTERNAL_OPT_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_INTERNAL_MAX_SELF_ANY=100 PRIMER_INTERNAL_MAX_SELF_END=100 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=30 PRIMER_INTERNAL_MAX_GC=100 PRIMER_INTERNAL_MIN_GC=0 PRIMER_PICK_INTERNAL_OLIGO=1 P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_23 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=1,1 P3_COMMENT=should succeed with warning (internal oligo excluded region outside of included region) = SEQUENCE_ID=boundary_test_24 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=2,1 P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_25 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=0,3 P3_COMMENT=should succeed with warning (internal oligo excluded region outside of included region) = SEQUENCE_ID=boundary_test_26 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=29,1 P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_27 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=30,1 P3_COMMENT=should fail w/ no legal internal oligo = SEQUENCE_ID=boundary_test_28 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=54,1 P3_COMMENT=should fail w/ no legal internal oligo = SEQUENCE_ID=boundary_test_29 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=55,1 P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_30 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=81,1 P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_31 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=82,1 P3_COMMENT=should generate warning (external oligo excluded region outside of included region) = SEQUENCE_ID=boundary_test_32 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=81,2 P3_COMMENT=should generate warning (external oligo excluded region outside of included region) = SEQUENCE_ID=boundary_test_33 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 P3_COMMENT=should succeed (testing number of internal oligo excluded regions) = SEQUENCE_ID=boundary_test_34 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN SEQUENCE_INCLUDED_REGION=2,80 SEQUENCE_INTERNAL_EXCLUDED_REGION=81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 81,1 P3_COMMENT=should fail (testing number of internal oligo excluded regions) = primer3-2.3.6/test/oligotm.txt0000664000077300007730000000421612231156245015253 0ustar ioanaioanaSEQ TM_MODEL SALT_CORRECTION SALT_CONC DIVALENT_CONC DNTP_CONC TM AAGGCGAGTCAGGCTCAGTG 1 2 5 1.5 0 62.123401 AAGGCGAGTCAGGCTCAGTG 1 2 0 1.5 0 62.123401 AAGGCGAGTCAGGCTCAGTG 1 2 10 1.5 0 62.895198 AAAGAAAGACCCAGTGATATGCTGTCT 0 0 50 0 0 63.684650 ACAACCCAGGCCCTGTGGAC 0 0 50 0 0.8 66.783376 ACTGGGATGACAGCAAGGCCC 0 0 50 0 1 67.947254 ACTTTCCTGTTGGACAAGCCCTTT 0 0 50 0 10 65.349952 AGCCTGGCCAACATGGTGAA 0 0 50 1 0 74.932401 AGGCTGAGGCGGGAGAATCA 0 0 50 1 0.8 71.934895 ATCTTGCCCGCCTCCACTTC 0 0 50 1 1 65.948961 CAACAAACCGTGGCCCTCTC 0 0 50 1.5 0 74.997943 CAAGGCCAGAGAATGGTTTCCAA 0 0 50 1.5 0.8 74.456879 CACTTTGGGAGGCCGAGGAG 0 0 50 2.5 0 77.815533 CAGTGGAAGTGACGCCGTGTG 0 0 50 2.5 0.8 77.633141 CCAAGCCCTTGGGCAGCTC 0 1 50 0 0 73.707812 CCACCTCAGCCTCCCGAGAA 0 1 50 0 1 72.289173 CCCAAAGCAATCTACAGATTCAAAGAA 0 1 50 0 0.8 68.920648 CCCGCGTGTGGTTTCTCTATTTG 0 1 50 0 0 71.826149 CCTCCCGGGTTCACACCATT 0 1 50 1.5 0 79.028727 CCTTCTGACAAGGGATTAATAACCAGA 0 1 50 2.5 0.8 75.999617 CGCAAGCCACCACTGTCCAT 0 2 50 0 2 72.669877 CTCGGTTATCCCTGGAATCTGGA 0 2 50 1 0 77.619980 CTGCCTGCACCCGCTTGTTT 0 2 50 1.5 0.9 80.472263 CTTCATGATCCACTGGCTATTACGG 0 2 50 2.5 1.0 76.728968 GAAACCTCACCCGGAGCCAGTA 1 0 50 0 2 51.910898 GACAACACCTGGACACCGGTTA 1 0 50 0 10 50.261568 GAGGCAGAGAGAGAGGAAGAGACAGA 1 0 50 0 0 52.569759 GATCCTCGGGCAGGCAGTTG 1 0 50 1 0 59.683669 GCAAGACAAAGGCATGCTTACAAGA 1 0 50 1.5 1 57.513748 GCACAGTGGCCCTTGTGACTG 1 0 50 2.5 1.0 61.599017 GCAGCCTGGACATAGCCCTGTA 1 1 50 0 0 58.108426 GCAGGCCAGAAGGCAGGATT 1 1 50 0 5 56.916950 GCCATGGACGTGGTCTACGC 1 1 50 0 0 57.801174 GCGACCCAAGCGAGTTACCA 1 1 50 1 0 62.957534 GCTTCATGTCAGATTTGTTATACCACTGGG 1 1 50 1 1 58.091721 GGCAGGCAGTTGGTCGGTGT 1 1 50 1.5 1.5 59.552734 GGGCCATGGGATTAGTCTGC 1 1 50 2.5 0 62.463700 GGTATGGTGTTTTAATACCTGTGGCAGA 1 1 50 2.5 1 64.204218 GTGAAACTCACGGTCACGCAAA 1 1 50 2.5 0 64.004323 TCACGCTCACAAACGAGAGGAAG 1 2 50 0 1 56.755143 TCCCTTGCCTCGTTCAGTGC 1 2 50 0 0 57.675190 TCGGCCTCCCAAAGTGCTG 1 2 50 0 1.5 57.961561 TCTCGCCATTTTGGTCTTGCAG 1 2 50 1 0 62.162400 TGATCAGCACAGTGGCTCACG 1 2 50 1.5 0 63.699372 TGCCGTTCACGTCGTAGCTG 1 2 50 1.5 0.8 63.844611 TGGGCCTCCCAAAGTGCTG 1 2 50 2.5 0.1 63.716743 TTGCATGCCCAGAGTTGACG 1 2 50 2.5 1 61.900322 primer3-2.3.6/test/primer_position_penalty_formatted_output0000664000077300007730000061506212231156245023435 0ustar ioanaioanaPRIMER PICKING RESULTS FOR AT3_X1 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 279 19 60.60 57.89 2.00 1.00 7.00 CCCTGTGGAAGATTAGCGG POSITION PENALTY, QUALITY: 0.000000, 1.599465 RIGHT PRIMER 361 19 60.58 57.89 6.00 0.00 9.00 GGGAAAGCTCACCCCTCTT POSITION PENALTY, QUALITY: 0.000000, 1.580558 SEQUENCE SIZE: 641 INCLUDED REGION SIZE: 641 PRODUCT SIZE: 83, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 298,45 0 TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTC 60 CCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACT 120 TTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCT 180 CTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCA 240 GGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCC >>>>>>>>>>>>>>>>>>>** 300 ATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTC *******************************************<<<<<<<<<<<<<<<<< 360 CCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAAGGACTCACAGGAATGACCTCCAA << 420 CACCTTTGAGAAGACCAGGCCCTCTCCCTGGTAGTTACAGTCAAAGACCTGTTTGGAAGA 480 CGTCATTTCAAGTGCTCTCCCTCCCACCCCACCTCTTGGGGTAAGGCCTTTCCTAAGCTA 540 CCCCTTGGGTCCCTAGCCTAAGAAACAAGGGGGATGTCATCCCTGGTGTAAAGATGCTGT 600 GCAGGAAGTCAGCACTCACGGGATCCAGGGGACGCTCCAAG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3504 0 289 0 0 16 0 818 1648 0 0 0 0 0 733 Right 3504 0 289 0 0 0 0 655 1723 0 0 0 0 0 837 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR AT3_X2 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 61.15 45.00 3.00 2.00 10.00 TTGGCATTTTGTCTCCTTGC POSITION PENALTY, QUALITY: 0.000000, 1.148919 RIGHT PRIMER 688 19 60.79 57.89 5.00 3.00 9.00 GGCAGAAGACCTTTGGTCG POSITION PENALTY, QUALITY: 20.000000, 21.785193 SEQUENCE SIZE: 967 INCLUDED REGION SIZE: 967 PRODUCT SIZE: 411, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,371 0 AAACAGGAAGCTTCTCATTCTTTTTTCTCCCAGCGTGGGGAATTGAGCACATAATACTCC 60 AAATAACCATCAGATGATTCTAATTCCAACATGACCACGTCCAGGCAACTGAACTGTCCC 120 CTGGCAAGAAGTCTAGGACTGAACCTGTCCCGGGCCCCTGTACTTGGTTCAAAGGATTTA 180 GCCTTTCTCTTGGCCACACCAGGTGGGCTGGAATCCTCTGCTTTACTGGGGCAACCCTGT 240 GGTGGGCAGTGGGGCTAGGGGTTGCAGCCTAGCTTAACTTGGCATTTTGTCTCCTTGCAG >>>>>>>>>>>>>>>>>>>>** 300 GAAGGTTTATCTTTTGTCCTTGCTGCTCATTGGCTTCTGGGACTGCGTGACCTGTCACGG ************************************************************ 360 GAGCCCTGTGGACATCTGCACAGCCAAGCCGCGGGACATTCCCATGAATCCCATGTGCAT ************************************************************ 420 TTACCGCTCCCCGGAGAAGAAGGCAACTGAGGATGAGGGCTCAGAACAGAAGATCCCGGA ************************************************************ 480 GGCCACCAACCGGCGTGTCTGGGAACTGTCCAAGGCCAATTCCCGCTTTGCTACCACTTT ************************************************************ 540 CTATCAGCACCTGGCAGATTCCAAGAATGACAATGATAACATTTTCCTGTCACCCCTGAG ************************************************************ 600 TATCTCCACGGCTTTTGCTATGACCAAGCTGGGTGCCTGTAATGACACCCTCCAGCAACT ************************************************************ 660 GATGGAGGTACGACCAAAGGTCTTCTGCCCAGCCACCTTGTTAGGAGCACCTTTGGGCTT ********* <<<<<<<<<<<<<<<<<<< 720 CCATAGGCCCAAGTCCAATGATTCCTCAACCAACACTGCAGCCACTAGGGGCGCTCATTA 780 TGCATTACGATTCCCTTTGAACATCACTGTGTTATAATTCCCTTTGAAAATCATTTTTTA 840 AAAAATTAGCCAAGGAATCTTGGCTATCTACTTTTTAAATCCTGGTTTCCTCTTTTGAGC 900 ACCTTAAAATGGGGGAAGGCTTGTATCTTCTCTCAACTTCTTTTCAGTAATTCTTTCATC 960 TATATGT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 6612 0 289 0 0 1 0 1035 3921 0 0 0 22 0 1344 Right 6657 0 289 0 0 114 0 1327 3439 0 2 0 19 0 1467 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR AT3_X3 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 265 20 61.73 45.00 3.00 0.00 11.00 CCAAACCCACCACCATTTTT POSITION PENALTY, QUALITY: 260.000000, 261.732281 RIGHT PRIMER 538 21 59.61 52.38 5.00 2.00 8.00 CAGGGGTAACATCTGCAACTC POSITION PENALTY, QUALITY: 0.000000, 1.393460 SEQUENCE SIZE: 816 INCLUDED REGION SIZE: 816 PRODUCT SIZE: 274, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,220 0 CAAGAGGACTTTTCCCATTCCAGCAAGAAAACGTCTGTGTGTTGATCTAGAGGCGTTTAG 60 AGACTTTAGGTGGCAACCTAGTCTCTCTTTTTCCCTTTATCCTTCCTACCCTTCATTCTT 120 CTTTTATCCTTTTATTCATCAGAACACAAGAGTTGAGCATTTATGCTGTCCCAGGTACTG 180 TGCTTGAAGGAGTTAACAACTGAGGTGGCTATTAGTCAGAGACTGACCAGCATGTGCTCA 240 CCACCCATGTTAACTAGGCAGCCCACCAAACCCACCACCATTTTTTTTTGACTTCTATAG >>>>>>>>>>>>>>>>>>>> ** 300 GTATTTAAGTTTGACACCATATCTGAGAAAACATCTGATCAGATCCACTTCTTCTTTGCC ************************************************************ 360 AAACTGAACTGCCGACTCTATCGAAAAGCCAACAAATCCTCCAAGTTAGTATCAGCCAAT ************************************************************ 420 CGCCTTTTTGGAGACAAATCCCTTACCTTCAATGAGACCTACCAGGACATCAGTGAGTTG ************************************************************ 480 GTATATGGAGCCAAGCTCCAGCCCCTGGACTTCAAGGTGAGTTGCAGATGTTACCCCTGA **************************************<<<<<<<<<<<<<<<<<<<<< 540 CCTCCGAGTTCTTCCTCTCCACTCAGAGATTGAGGAGGTGGAGAAACAGCATCCAAATTC 600 ACACTGCTTTGCTGCTGAAGACTGCTGGAGGGCTGACTAAAAGTTAGAACCCCTGCAATA 660 GTTATTCTTACTTGAAACCTGAGAAATCAAAGGTATCCATGCTTGGATTGTAGTGACTGC 720 CCAGAAAACATGAATTAATAATCAATTCTTCATTCCATCCACCAACTTCAAATATATACC 780 AAAGGGTGTTTTGAAGATGCCAGTTCTACAAGATAT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 5081 0 289 0 0 13 0 1874 1352 0 0 0 25 0 1528 Right 5254 0 289 0 0 103 0 1706 1557 0 0 0 0 0 1599 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR AT3_X3' Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 265 27 60.24 37.04 4.00 0.00 11.00 CACAGGTGAGTAGGTTTATTTTCTGTT POSITION PENALTY, QUALITY: 120.000000, 127.241506 RIGHT PRIMER 458 19 60.21 57.89 4.00 3.00 9.00 CTCCAGGGCCATTCTGAGT POSITION PENALTY, QUALITY: 0.000000, 1.206859 SEQUENCE SIZE: 738 INCLUDED REGION SIZE: 738 PRODUCT SIZE: 194, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,142 0 AGTCAGAGAAAATAATATTTTATCTTCTTTTTCCCAGACTATTTTCGCCTTCCTTCTTTT 60 CATTTATTTCTTCCTATTTCTTTTTGTCTTTTTCTTCTGATAATATTTATTAACTACAGG 120 AAAGATTCATGGAACTATATTAGATATGTGAGGCTTCCCAATTTGGGTTAGAGCAATGGC 180 TTCTTAATCAAATGGTGGGAAAGGACAGAGGGATGGTGAGAAAAATAAAATGCTGCCTGG 240 GAAAATGGAGAAGCCAATTGAATAGCACAGGTGAGTAGGTTTATTTTCTGTTCTCCTCAG >>>>>>>>>>>>>>>>>>>>>>>>>>> ** 300 GAAAATGCAGAGCAATCCAGAGCGGCCATCAACAAATGGGTGTCCAATAAGACCGAAGGC ************************************************************ 360 CGAATCACCGATGTCATTCCCTCGGAAGCCATCAATGAGCTCACTGTTCTGGTGCTGGTT ************************************************************ 420 AACACCATTTACTTCAAGGTACTCAGAATGGCCCTGGAGAGACCCCAGGGACTTCCTCTT ********************<<<<<<<<<<<<<<<<<<< 480 GCTCTTCAGCTTACCCCCTTTTTTTTTAAATGGCGAGACCGAAGCCCTGAGAGGGCAAAT 540 GGACTGCCGAAAGCTACACAGGTACAGGTCAGCAGGGCAGGTCAATCTATTATTTATTTA 600 TTTATTTATTTTTGACAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATC 660 TCGGCTCACTGCAAGCTTCGCCTCCTGGGTTCTCGGCATTCTCCTGCCTCAGCCTCCCAA 720 GTAGCTGGGAATACAGGC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4474 0 289 0 0 273 0 1464 1424 0 0 13 0 0 1011 Right 4294 0 289 0 0 209 0 512 2472 0 0 0 25 0 787 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR AT3_X4 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 273 25 58.09 36.00 3.00 0.00 10.00 TGTTCTTACTTTGTGATTCTCTTCC POSITION PENALTY, QUALITY: 0.000000, 6.906112 RIGHT PRIMER 715 23 60.12 47.83 5.00 2.00 11.00 GGAGGAAACTCCTTCCTAGACAA POSITION PENALTY, QUALITY: 0.000000, 3.115306 SEQUENCE SIZE: 991 INCLUDED REGION SIZE: 991 PRODUCT SIZE: 443, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 298,395 0 GGCTTTGTCACTCATTTCTTCTTAGCATGGAATAGCATTACATTTGGTCTGGATGTACCA 60 CAGTCTGTCTATTCATCTACTGAAGGACATTTTGGCTGCTTCCAAGGTTTGACAGTTATG 120 AATAAACCTACTCATAATTCCATCATTCTGACACAGCCATTGTTAACCTTTTTGTGCATA 180 TCCCGCCAGTCTTTTTTCCGAATAATTATATATTAATGTAACACTATAATATGGATATGT 240 CTGTGTCAATAACTATCCTCCTATGAATGTTTGTGTTCTTACTTTGTGATTCTCTTCCAG >>>>>>>>>>>>>>>>>>>>>>>>>** 300 GGCCTGTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGGAACTGTTCTACAAGGCT ************************************************************ 360 GATGGAGAGTCGTGTTCAGCATCTATGATGTACCAGGAAGGCAAGTTCCGTTATCGGCGC ************************************************************ 420 GTGGCTGAAGGCACCCAGGTGCTTGAGTTGCCCTTCAAAGGTGATGACATCACCATGGTC ************************************************************ 480 CTCATCTTGCCCAAGCCTGAGAAGAGCCTGGCCAAGGTGGAGAAGGAACTCACCCCAGAG ************************************************************ 540 GTGCTGCAGGAGTGGCTGGATGAATTGGAGGAGATGATGCTGGTGGTCCACATGCCCCGC ************************************************************ 600 TTCCGCATTGAGGACGGCTTCAGTTTGAAGGAGCAGCTGCAAGACATGGGCCTTGTCGAT ************************************************************ 660 CTGTTCAGCCCTGAAAAGTCCAAACTCCCAGGTTTGTCTAGGAAGGAGTTTCCTCCCTTC *********************************<<<<<<<<<<<<<<<<<<<<<<< 720 TCCACCCGCAAGGTAGTCTGACCAAAAGTGGAAGAGTTGGAGAAAGAATAGAAAGGAGCA 780 ACAAGTCAGGACTCCTGGATACTGATCCTAGTTTCTACTGCTAATTTGTGGAAATCTCTT 840 TTCCTTTTGAGACCTCAGTTTCCTCTTCTGTAAAAGGGAAGTTTGTTCTTGGATCTCCAT 900 GGGCCCAGCCAGCACTGGTGCCCTGTGAGTCTGTATCAGGTAGAGGAGATGGGACCAGGT 960 GGAGAGGAATTTGAAAGGGCATTGGAATTCA KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 6872 0 289 0 0 226 0 1924 2932 0 0 0 17 0 1484 Right 7004 0 289 0 0 0 0 1493 3474 0 0 0 0 0 1748 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR AT3_X5 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 276 21 58.94 52.38 4.00 0.00 8.00 AGACTTCTCCGGTCTTCCTTC POSITION PENALTY, QUALITY: 20.000000, 22.061137 RIGHT PRIMER 389 22 58.28 50.00 4.00 2.00 11.00 AAGAGAGTGGGGAAGGTGTACT POSITION PENALTY, QUALITY: 20.000000, 23.722528 SEQUENCE SIZE: 665 INCLUDED REGION SIZE: 665 PRODUCT SIZE: 114, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 298,69 0 TAGAGACAAGGTTTCACCATATTAGTTAGAGTGTCTCCAACTCCTGACCTCAGGTGATCT 60 GTACACCTTGGCCTCCCGAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCCCC 120 AAAGTACTTTATTATTTTTAACACATATTCATTGTGAGAGTATGATTAGGTGAAGATTTA 180 GGATTTCTTCTTATGTTTCAAAAAGCCCCAAAGGATCTCTTAATCCAAACTGAATTCCCA 240 TCTGTGGATTGAAGCCAACTTTCTCCCATCTCACAAAGACTTCTCCGGTCTTCCTTCCAG >>>>>>>>>>>>>>>>>>>>> ** 300 GTATTGTTGCAGAAGGCCGAGATGACCTCTATGTCTCAGATGCATTCCATAAGGCATTTC ************************************************************ 360 TTGAGGTGAGTACACCTTCCCCACTCTCTTAGGGTACAGAAAGGAGATGCATGAACAGCA ******* <<<<<<<<<<<<<<<<<<<<<< 420 GGAACACGTGGAAAAGGCCTGTTTCCAGTGTTAAGGCATGCAAAAGGCCTCCACAGGCTG 480 CTATAATACAGCCCTCTCCAAAACCTTCATGGTGTGATTGTTCTGCCTTCCCTCCCACTA 540 CCTCTTCTGTAGCAGGTCAAGCGGGAACACAAACATTTAGGGAGGGTGATATAGGAAAAG 600 AAGCCAGCAAAGGCCATCAAGAAGAAATTTACAGCATGAGGAGAACCAGAAGAGTATGGG 660 GTCGC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3744 0 289 0 0 148 0 1249 1208 0 0 0 0 0 850 Right 3744 0 289 0 0 0 0 874 1504 0 8 0 0 0 1069 Pair Stats: considered 21, high end compl 20, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR AT3_X6 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 58.42 45.00 6.00 3.00 8.00 TTTATTCCCATGTGACCTGC POSITION PENALTY, QUALITY: 0.000000, 1.577239 RIGHT PRIMER 503 22 57.63 40.91 4.00 0.00 11.00 GGAAGAGGTGCAAAGAATAAGA POSITION PENALTY, QUALITY: 60.000000, 64.365428 SEQUENCE SIZE: 732 INCLUDED REGION SIZE: 732 PRODUCT SIZE: 226, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 298,181 0 CCATTTACAATGGTACCAGCCAGAAATGTGATAACCTGTGTACATTCAGATTTCTGGGTT 60 ACCTGAATGGAACTCTTACACTTATTACCTAGCACAAGGCTTGGACAAACACAAGTACCT 120 TACATTCTCTGCATGAAAGAATGAGTGAAAGTAGGATTCTGGAGGGAATCCAACCTGACC 180 CAAATGTACTTTTTACTGGAAAACAAAAGCATTTGAGGAATTGCTGTGTCTGTGGATGAT 240 TTACCTGCCAAAATGAACGGCAGAGTGGCTAATTTAGTTTTATTCCCATGTGACCTGCAG >>>>>>>>>>>>>>>>>>>>** 300 GTAAATGAAGAAGGCAGTGAAGCAGCTGCAAGTACCGCTGTTGTGATTGCTGGCCGTTCG ************************************************************ 360 CTAAACCCCAACAGGGTGACTTTCAAGGCCAACAGGCCTTTCCTGGTTTTTATAAGAGAA ************************************************************ 420 GTTCCTCTGAACACTATTATCTTCATGGGCAGAGTAGCCAACCCTTGTGTTAAGTAAAAT *********************************************************** 480 GTTCTTATTCTTTGCACCTCTTCCTATTTTTGGTTTGTGAACAGAAGTAAAAATAAATAC <<<<<<<<<<<<<<<<<<<<<< 540 AAACTACTTCCATCTCACATTATAAATGGACTCTGCATTTGAAATGAAGACAAGGAAAGG 600 GGAAACATGCTATTGGGGCACATGGTAAAATGATGCCTTCAAGTTGTTCTTTACCCAGTA 660 ACCACATCTGGATCAAGAAAATGAGGGAGAGAGCGATAAAAGATGGTAGACAGCCAGAAA 720 GGGAAGGGAGAG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4819 0 244 0 0 0 0 1696 1512 0 0 0 0 0 1367 Right 4414 0 289 0 0 103 0 1578 1288 0 0 14 0 0 1142 Pair Stats: considered 3, high end compl 2, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X1 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 276 22 57.84 45.45 3.00 2.00 10.00 CTTACACACCACTGCCTGATAA POSITION PENALTY, QUALITY: 0.000000, 4.158608 RIGHT PRIMER 442 23 60.70 47.83 6.00 2.00 9.00 GGCAGACAGATGCACTGATACTT POSITION PENALTY, QUALITY: 0.000000, 3.704304 SEQUENCE SIZE: 718 INCLUDED REGION SIZE: 718 PRODUCT SIZE: 167, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 298,122 0 AGTAAAAATAAATGAATTCATGGAAACATATTAAGCAATTATCCAGATAACATAAGGGAT 60 GGCAAAAATGGTGCAGATGGTGGAGGGGAGACAAGTAGAAGTTGGGGTGCTCTTGTTGAA 120 TGTCTGGCTCTGAACTCTAGAGGAGGCCGCAGGGGCTGGGCAGGAAGGAGGTGAATCTCT 180 GGGGCCAGGAAGACCCTGCTGCCCGGAAGAGCCTCATGTTCCGTGGGGGCTGGGCGGACA 240 TACATATACGGGCTCCAGGCTGAACGGCTCGGGCCACTTACACACCACTGCCTGATAACC >>>>>>>>>>>>>>>>>>>>>>** 300 ATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAA ************************************************************ 360 GGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTGT ************************************************************ 420 AAGTATCAGTGCATCTGTCTGCCCTGCCAGGGGTCTTTTCATGGACACCCACTATGCCAG <<<<<<<<<<<<<<<<<<<<<<< 480 GAGCCTCCCTGGCCTGAAGCCAGCCCTGAAGCCGGCTGCCACACTAGCCCAGAGAGAGGA 540 GTGCCCTGGGAGGGAGATGGGCTGAGTGGAGCTGTCATCACCCCCTCCTGACCTCGCCTT 600 CAAGGTCAAGTTCTTTGGTGAGAAGGTCCTAGCTGCATTGCAAACAGCCAGGTATAGGGA 660 TTTGTGTTTGTCTGCGACCCAGAATCACTGGGGTTCGAGTTAGGGTTCAGATCTGAGC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4274 0 289 0 0 68 0 569 2779 0 0 0 0 0 569 Right 4274 0 289 0 0 0 0 375 2978 0 0 0 0 0 632 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X2 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 280 18 59.59 61.11 3.00 0.00 8.00 ATCCACTGCCCTCCCTCT POSITION PENALTY, QUALITY: 0.000000, 2.407131 RIGHT PRIMER 443 18 62.34 66.67 3.00 2.00 8.00 AGCCAGGTCACACCCGAG POSITION PENALTY, QUALITY: 180.000000, 184.341886 SEQUENCE SIZE: 549 INCLUDED REGION SIZE: 549 PRODUCT SIZE: 164, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 298,119 0 CTAGGGTTAGTGTGCGGCTGGGCTCAGGGCTGCCCCATTTGCTAGGATCGTGGGGTTCCC 60 ATGTGTCAGGATCCAGAGGCTAGGGTATGATCAGGATCTCTAGCTGGGGTCAGGGTCAGA 120 GCTCTCTGTGTCCCCTAGAATTGCCATCAACCTTAAACCCAGAGGAGGCCCAGTCCAACC 180 CCTCAGCTTTAAGACCTGGGAGCCTCATCTCAGAGAGGCTGAGTCATGGCCAAGGCAGTT 240 GGGGTGGGAGCAGGGGGCTTGGTGTGGGCCTGCAGCCCTCATCCACTGCCCTCCCTCTAG >>>>>>>>>>>>>>>>>>** 300 TGAACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATA ************************************************************ 360 TCACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAAGTGAG ********************************************************* 420 TCGGGCCTCGGGTGTGACCTGGCTGGGGGTAGGGTGGCGGGAGGAACAGCCTGGGCTTCC <<<<<<<<<<<<<<<<<< 480 CCCAGCCACAGGGAGGAAAGGCAGCAGCTGGGGGACTCAGGTCTCTCCCCTTGATTTGGA 540 ACCAGAGCC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4078 0 123 0 0 7 0 374 2820 0 0 0 0 0 754 Right 2584 0 289 0 0 1 0 95 1901 0 0 0 0 0 298 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X3 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 60.82 55.00 2.00 0.00 9.00 CCTCACTTCCATTCCTTCCC POSITION PENALTY, QUALITY: 0.000000, 0.821928 RIGHT PRIMER 457 21 60.41 47.62 8.00 2.00 9.00 CATCAGCTGACAGGAATGCTT POSITION PENALTY, QUALITY: 0.000000, 1.412222 SEQUENCE SIZE: 735 INCLUDED REGION SIZE: 735 PRODUCT SIZE: 180, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 298,139 0 TGCCTCCTCTTCCACTTTTCGGTACCCTGTGATTGATTGGGACCACCCAGATAACCTAGG 60 ATCATCTCCCCACCTACCCCAAGGTCCTTAACTTAACCATACTTCATATGGGTAACACGA 120 GTTGAGTGTGGTACCCAGGTTTGACATGTTGGGTAACATATTTGCAGGTTCTGTGGATTA 180 GGAGGACATTTTGGGGGCCATGATTCTATCTTCCACCCTCGCCTAGACAAAATTGGAGGC 240 TCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAG >>>>>>>>>>>>>>>>>>>>** 300 CATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTC ************************************************************ 360 CATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTA ************************************************************ 420 CACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTG *****************<<<<<<<<<<<<<<<<<<<<< 480 GGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTC 540 CATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTG 600 TCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAG 660 GTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGT 720 TGGGGACCCCAGAGC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4444 0 289 0 0 0 0 844 2220 0 0 0 0 0 1091 Right 4444 0 289 0 0 0 0 400 2950 0 0 0 0 0 805 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X4 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 280 18 59.59 61.11 3.00 0.00 9.00 GGGTCCTTGGCTCTTTCC POSITION PENALTY, QUALITY: 0.000000, 2.413192 RIGHT PRIMER 392 20 61.28 55.00 3.00 2.00 9.00 CCAGAGGACGCTTGACACAT POSITION PENALTY, QUALITY: 0.000000, 1.279241 SEQUENCE SIZE: 671 INCLUDED REGION SIZE: 671 PRODUCT SIZE: 113, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,75 0 CTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTC 60 ACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGA 120 GCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGG 180 GACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCC 240 ATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAG >>>>>>>>>>>>>>>>>>** 300 GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAA ************************************************************ 360 CACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTT *************<<<<<<<<<<<<<<<<<<<< 420 GGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGC 480 CTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCA 540 GCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAG 600 CCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGC 660 TGCTCCTGCAT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3804 0 289 0 0 0 0 382 2360 0 0 0 0 0 773 Right 3804 0 289 0 0 0 0 348 2565 0 0 0 0 0 602 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X5 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 280 18 62.73 66.67 4.00 2.00 8.00 CATGTCGGGTCTCCCTGC POSITION PENALTY, QUALITY: 0.000000, 4.729227 RIGHT PRIMER 409 20 62.18 60.00 4.00 0.00 8.00 GGGCACAGGGTGGTGTACTT POSITION PENALTY, QUALITY: 0.000000, 2.179675 SEQUENCE SIZE: 688 INCLUDED REGION SIZE: 688 PRODUCT SIZE: 130, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,92 0 GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAA 60 CACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTT 120 GGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGC 180 CTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCA 240 GCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAG >>>>>>>>>>>>>>>>>>** 300 CCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGC ************************************************************ 360 TGCTCCTGCATCTCCAAGGGGAGCGAGAGTAAGTACACCACCCTGTGCCCCCATTCCTGT ******************************<<<<<<<<<<<<<<<<<<<< 420 CGTGCCCATCCTGTTAGTGTGTCCACGGCCCCCTCCAGGCTCAACCCCACACAGGGATGC 480 TTGTGGGTGGCCAAACCTGAGGGCAGCAATACCTTCAGTGGGGTCATTCCATCCCCCTCC 540 ATCAATACACCCTAAAGGCTGGAAACAACAATAACCAACAGCTAGTAACTAACAGCTATT 600 AAGAACTTCTGTTGGCAAAGCACTATTCCAAGCCCTTTCATGAATTAATTGATTTTGTCC 660 TTAAAACCAACCCTAGGATATAGATTCT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3974 0 289 0 0 0 0 348 2707 0 0 0 0 0 630 Right 3974 0 289 0 0 34 0 889 1971 0 0 0 0 0 791 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X6 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 276 18 59.99 55.56 3.00 3.00 9.00 CATTGTGGTGCTTGCTGC POSITION PENALTY, QUALITY: 80.000000, 82.014189 RIGHT PRIMER 395 19 62.38 63.16 3.00 3.00 9.00 ACAGGGAGTCAGCCAGCCT POSITION PENALTY, QUALITY: 100.000000, 103.379680 SEQUENCE SIZE: 670 INCLUDED REGION SIZE: 670 PRODUCT SIZE: 120, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 298,74 0 TTTCTGACTCTGGAGCAAGACGGATACATGTATGAATTTGGACTCTAGACACGTTCTCGT 60 GTGTGTGACAGGTGTGAGCGTCACAGGAGCTGGGCCCTCCCGAGGAATTCTGGATGGTGC 120 CACAGTTAATTCTTGGGTCTGAGGCTCCGTGTTCTCACTGCAAAATGGGAGTGATAATTC 180 TTACTTCCTGAGCTACAAGAGTCAGGGCCAACAGAGCCATGAAGGAGCCTGGTACACACT 240 AGGCGCTCCATGGATGCACAGGACTGGTCAGGGGCTCATTGTGGTGCTTGCTGCCTTCAG >>>>>>>>>>>>>>>>>> ** 300 GCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCT ************************************************************ 360 GAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCT ************ <<<<<<<<<<<<<<<<<<< 420 GGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGC 480 CAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCG 540 TCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGA 600 TCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTT 660 CTGTCTGCAT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3794 0 289 0 0 0 0 681 1966 0 0 0 0 0 858 Right 3794 0 289 0 0 0 0 274 2616 2 3 0 0 0 610 Pair Stats: considered 40, high end compl 38, ok 2 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X7 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 271 20 60.59 55.00 6.00 2.00 8.00 CTGGAAAGCACCTGCTCTGT POSITION PENALTY, QUALITY: 140.000000, 140.588967 RIGHT PRIMER 382 20 59.04 50.00 4.00 0.00 10.00 ATGCAGACAGAAACGCACTC POSITION PENALTY, QUALITY: 0.000000, 0.959447 SEQUENCE SIZE: 661 INCLUDED REGION SIZE: 661 PRODUCT SIZE: 112, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 298,65 0 TTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCC 60 TGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCA 120 TGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACC 180 TTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGA 240 AGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAG >>>>>>>>>>>>>>>>>>>> ** 300 ATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCT ************************************************************ 360 GGTGAGTGCGTTTCTGTCTGCATGCCTCAGAAGACAGCAGTGGGAGCCAGAAAGCCACCT ***<<<<<<<<<<<<<<<<<<<< 420 GCTGCACTATGTGGCCTTGGGACTGTCACTCTTCCTGTCTAGGTCCCATGGGCTCTATCT 480 GGCTCTGACACTTGATGATTAGTTATGAGCATACTTTGGCAAAGCTCTGCCCCTTTGGTG 540 CGGCTCACAAGCTGTGTGGCGAAGGGCTTGTCTATAGAACTCAGGACAAATGGGTGATTA 600 AGTCCAAGAGGCATCCAAGATTCTCCTGGAAGTAGATTAGGAAAAAAGATAATTAGATTG 660 C KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3704 0 289 0 0 0 0 262 2572 0 3 0 0 0 578 Right 3593 0 289 0 0 15 0 752 1654 0 0 0 22 0 861 Pair Stats: considered 102, high any compl 81, high end compl 20, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X8 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 59.81 50.00 4.00 2.00 10.00 CGTTGATCTTTTCCTCCTGC POSITION PENALTY, QUALITY: 0.000000, 0.186600 RIGHT PRIMER 412 19 62.33 63.16 1.00 0.00 10.00 CCACCCTCCCACAACTCCT POSITION PENALTY, QUALITY: 0.000000, 3.327467 SEQUENCE SIZE: 692 INCLUDED REGION SIZE: 692 PRODUCT SIZE: 135, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,96 0 GCTGGGCACTCATCCATGTACTGTACTCTCCTATGCAGTACAGAGCAGAGCTGGGTTTCA 60 GCCCAAGTCTTGGACTCTGCTCTGAACCAACCTTCTAGAAGGGCTCTACCTACCCAGACA 120 GACAGACTTGGGAAAAGAGAGAATGAAAAAGTGCCACACCCCTCCCCGCACACCCAGGTC 180 CCACTTTACAGAGGGGAACACTGAGGCTGGAGGGTTGGGTAGCTGTGTGGATGCAGGGGA 240 CGGTGACTCAGGGCAATTCCCCCATCCCTGAGGCCCTGCGTTGATCTTTTCCTCCTGCAG >>>>>>>>>>>>>>>>>>>>** 300 CCAGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCA ************************************************************ 360 TCACAGCCTCCTACCTGGAGTCCCATCACAAGGTAGGAGTTGTGGGAGGGTGGGCAGGGC **********************************<<<<<<<<<<<<<<<<<<< 420 CCAGCTTCCCCAGGGGAGTTGGTCCTTTTTTGTGCTCTGACAACCCCATCCCCCAGCTTC 480 AACCTTATGGCAGCCAAGAGTCCTGGGGAGCTCCTCCTCATTCCTGATGCTCCTCCGCAT 540 TCCTGATGCTGCGAGGAGGGCAGGCCACAGCGACGTGCCCCTGACCCCTCTCTGCAGGCA 600 CCAGGGCTGCCCACTACAAGGATCCCAGCAAAGCACCAGCTCCTTCCTAGAGGGCTTATT 660 CGGCTTCTGTCATCCTCTACAGCAGTGGATTG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4014 0 289 0 0 0 0 645 2223 0 0 0 0 0 857 Right 3861 0 289 0 0 8 0 269 2629 0 0 0 22 0 644 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X9 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 145 18 62.86 66.67 3.00 0.00 8.00 AGCGTCCTGGCTTCCTCC POSITION PENALTY, QUALITY: 0.000000, 4.864632 RIGHT PRIMER 375 18 62.63 66.67 4.00 2.00 8.00 ATCCCCTAGCAGCCCAGC POSITION PENALTY, QUALITY: 220.000000, 224.633226 SEQUENCE SIZE: 628 INCLUDED REGION SIZE: 628 PRODUCT SIZE: 231, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 163,184 0 TTTGTTGAATGAGTGAAAGCCGCTGGGGAAACTGGGTACAGCTCTTTCCTCAGTTTCCCC 60 ATCTGCACTCTGGGCTGAATGCTGGGCTCCTCCCAATCTCCCTGAAGCTGGACCTGAGCC 120 CAGTAGGGACACACAGGGTCCAGCCAGCGTCCTGGCTTCCTCCAGGGTCATTTCATCTAC >>>>>>>>>>>>>>>>>>***************** 180 AAGAATGTCTCAGAGGACCTCCCCCTCCCCACCTTCTCGCCCACACTGCTGGGGGACTCC ************************************************************ 240 CGCATGCTGTACTTCTGGTTCTCTGAGCGAGTCTTCCACTCGCTGGCCAAGGTAGCTTTC ************************************************************ 300 CAGGATGGCCGCCTCATGCTCAGCCTGATGGGAGACGAGTTCAAGGTGAGTGGGTGGGGC *********************************************** << 360 TGGGCTGCTAGGGGATCCAGATGGCATGTGGTATGTGTGTGTGTGCACACGCATGGGGAG <<<<<<<<<<<<<<<< 420 GAGGGAGGAAACTCGGAAACTTGGTGGTGGGCAAAAGAACTAAGCTGGAGCAATAGCAGT 480 GAAGTCCAGACTGGGCACAGTGGCTCACACCTGTAATCCCAATCCTTTGGGAGGCTGAGA 540 TGTAGCAGGACGAACCGCAGACAAAACTCCTCAGACACTGAGTTAAAGAAGGAAAGAGTT 600 TATTCAGCCGGGAGCATGGGTAAGACTC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3527 0 272 0 0 0 0 323 2355 0 0 0 0 0 577 Right 4589 0 154 0 0 0 0 711 2838 0 0 0 0 0 886 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X10 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 213 22 57.47 36.36 3.00 0.00 10.00 TCCAACTTCCTCATTTCTTTTC POSITION PENALTY, QUALITY: 0.000000, 4.533349 RIGHT PRIMER 319 19 61.92 57.89 4.00 2.00 9.00 ATAAACCCCACACAGGGGC POSITION PENALTY, QUALITY: 220.000000, 222.919106 SEQUENCE SIZE: 399 INCLUDED REGION SIZE: 399 PRODUCT SIZE: 107, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 235,55 0 CTTGTCGAGGTCACACAGCATGTGTGGGGCACAGTGGGGAGCAGAAGCCAGCCTCCAGCC 60 GGACACCCCTTCCCCATTCCACACCCTGCCCAGAGCATCTCACATGTTGTCTGGGAGGTT 120 GGGAGTTGCGTCTGAGGAGGGGTCCAGTCCTTGAAACTGCCCTTGGTCCCTGCGAAGTTT 180 TCTTCTGAGGAGTGGACTTTACTCCACCCACCCTCCAACTTCCTCATTTCTTTTCAGGCA >>>>>>>>>>>>>>>>>>>>>>***** 240 GTGCTGGAGACCTGGGGCTTCAACACCAACCAGGAAATCTTCCAAGAGGTAACTCCCCCC ************************************************** 300 TGCCCCTGTGTGGGGTTTATCTCACGTACCCCAATCCTGCTCTGGCTTCAAGAGCCCCCA <<<<<<<<<<<<<<<<<<< 360 CACAGCCAATAACACCACCAATGGCAACAATTATAACAG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 2785 0 100 0 0 0 0 259 1906 0 0 0 0 0 520 Right 1498 0 226 0 0 0 0 153 792 0 0 0 22 0 305 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X11 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 107 18 60.31 66.67 5.00 0.00 9.00 GGGACCCCTGTCTTCCAC POSITION PENALTY, QUALITY: 0.000000, 2.311131 RIGHT PRIMER 317 18 61.12 66.67 4.00 0.00 10.00 ACCACCTCTCCCTGCACC POSITION PENALTY, QUALITY: 120.000000, 123.122481 SEQUENCE SIZE: 409 INCLUDED REGION SIZE: 409 PRODUCT SIZE: 211, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 125,169 0 CCCCGAGCTACTTCCTTTTCCCAGCCCTGGGGCCCGGAGCCAGCTTTGTGGTTCCCATCT 60 CCGAGGGCATGGACTGCTGCTCCGTGATGGGCCCCTGTCCTGGCCATGGGACCCCTGTCT >>>>>>>>>>>>> 120 TCCACAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTCCACTGCCTCAAGAT >>>>>******************************************************* 180 GCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCAGTGATGGTGAAATT ************************************************************ 240 CCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTTGAAGAGGTGAGGCG ****************************************************** 300 GGTGCAGGGAGAGGTGGTGGTGGGGGAACTGACTCACATATGGGCCGCAGAGGGCAGGGG <<<<<<<<<<<<<<<<<< 360 CTGGGGGTCTCTGAAGCCTCCAGATCCTTCTCACCACCTCTGCTGGCAC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 2831 0 106 0 0 50 0 286 1982 0 0 0 0 0 407 Right 2741 0 116 0 0 42 0 336 1775 0 0 0 0 0 472 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X12 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 58.48 45.00 8.00 0.00 11.00 CAAATTTGGTTTCTCTCCCC POSITION PENALTY, QUALITY: 0.000000, 1.522783 RIGHT PRIMER 391 22 61.09 50.00 6.00 3.00 10.00 CCTTCTCTTCTCTGCAGCACAT POSITION PENALTY, QUALITY: 0.000000, 3.085335 SEQUENCE SIZE: 668 INCLUDED REGION SIZE: 668 PRODUCT SIZE: 114, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,72 0 GTTGCAGTGAGCTGAGATCGTGCCACTGCCCTCCAGCCTGGGCAACAGAGTGAGACTGTC 60 TCAAAAACAAAAAAAGAAAAGAAAAGAAAAAGAAAGTGACTTCTCAGGTCCTAACCCCAA 120 AGCCACAGGTGCTGGGGAACTTTCCTCGGTTTTCAGAAGAGCAGTAGCTAAGCCTGGTTC 180 CCGTGTCATCCTTGCCTCTCCAGTCCCTCAGTGGAAAGAATCAGGGGCCCTGAGCTAGGA 240 GGGTTGCTCTCTGCTTCGGGAAGAGCCCTGGCTCACAGCAAATTTGGTTTCTCTCCCCAG >>>>>>>>>>>>>>>>>>>>** 300 GATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGCCTCTTG ************************************************************ 360 GATTTCCAGTATGTGCTGCAGAGAAGAGAAGGGGGCGGTCAACTCCGCAAACCTCTCCCT **********<<<<<<<<<<<<<<<<<<<<<< 420 GGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCT 480 GGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGG 540 AAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAA 600 AAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAA 660 TGGAGGGT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3686 0 289 0 0 114 0 669 1809 0 0 0 14 0 791 Right 3774 0 289 0 0 0 0 619 2094 0 0 0 0 0 772 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X13 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 269 23 60.53 34.78 4.00 0.00 9.00 GGGTCAAATTATCATCGCTTTTT POSITION PENALTY, QUALITY: 120.000000, 123.526785 RIGHT PRIMER 358 23 59.90 52.17 6.00 0.00 8.00 CCCCAGTCTATCCAAGACTACCT POSITION PENALTY, QUALITY: 0.000000, 3.097964 SEQUENCE SIZE: 634 INCLUDED REGION SIZE: 634 PRODUCT SIZE: 90, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,38 0 CGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGG 60 TGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACC 120 CTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAG 180 GAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCA 240 GGGGATGTTACAGGAGGGGGCCCAATGGAGGGTCAAATTATCATCGCTTTTTTATTTCAG >>>>>>>>>>>>>>>>>>>>>>> ** 300 GATTACACCAAAGACTGTTTCCAACTTGACTGAGGTAGGTAGTCTTGGATAGACTGGGGG ************************************<<<<<<<<<<<<<<<<<<<<<<< 360 AAATAAGTCCTGTGGGACCTCCTGCCTTAAAGAAAGCAGGCGGAGGGCCCTAAAGGAAAT 420 CAGGCAACCAGACCAAAAGAATGTGACCAGGTGGTCCATGCTGTGTCTCTTGTGACCCTT 480 CTTCTCCCTGCCATGTCTTTTGGGAGAGCCCTTGTGTTGCAAAAATGAGAGTGTGGTGGT 540 ATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTT 600 CCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3282 0 289 0 0 1 0 523 1815 0 0 0 22 0 632 Right 3434 0 289 0 0 0 0 586 1682 0 0 0 0 0 877 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X14 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 19 60.74 47.37 6.00 2.00 9.00 CACCATGGGCATTTGATTG POSITION PENALTY, QUALITY: 20.000000, 21.740693 RIGHT PRIMER 396 18 62.83 66.67 2.00 0.00 8.00 GTTTCCCCTCCAGCCCAC POSITION PENALTY, QUALITY: 80.000000, 84.832325 SEQUENCE SIZE: 436 INCLUDED REGION SIZE: 436 PRODUCT SIZE: 119, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,77 0 GTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAA 60 GCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTCC 120 CCAGGGCGAAGGAAAGACCACTCAGGAAGGGCACCGTCTGGGGCAGGAAAACGGAGTGGG 180 TTGGATGTATTTTTTTCACGGATGGGCATGAGGATGAATGCTTGTCCAGGCCGTGCAGCA 240 TCTGCCTTGTGGGTCACTTCTGTGCTCCAGGGAGGACTCACCATGGGCATTTGATTGCAG >>>>>>>>>>>>>>>>>>> ** 300 AGCAGCTCCGAGTCCGTCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCT ************************************************************ 360 GAGGTCATGTCTCGTAAGTGTGGGCTGGAGGGGAAACTGGGTGCCGAGGCTGACAGAGCT *************** <<<<<<<<<<<<<<<<<< 420 TCCCATTTCACCTTTT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3425 0 52 0 0 3 0 235 2489 0 2 0 23 0 621 Right 1454 0 289 0 0 0 0 83 882 0 0 0 0 0 200 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X15 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 231 18 62.06 66.67 4.00 1.00 9.00 ACTGGGGCTCTGTCCCCT POSITION PENALTY, QUALITY: 100.000000, 104.061036 RIGHT PRIMER 377 18 61.67 61.11 3.00 1.00 11.00 AGGAACAGGGGCTGGTGA POSITION PENALTY, QUALITY: 320.000000, 323.670770 SEQUENCE SIZE: 642 INCLUDED REGION SIZE: 642 PRODUCT SIZE: 147, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 254,90 0 GGATGGGTTGGGAGCTCAAGTTTTGGGGCAGAAGGGAATTTTTTTTGGCAGCAGAGTGCA 60 AGCCCTGCCGCCAGGCAAACTCTGCTCTTCCTCATCCTCAGAAGCACTTGCTCACTCTGC 120 TAAATCAAAGTGAAACGCATGTTTACAGAATATTGGTCCAAAAGGGTCTCAGCATCTCCC 180 ACTACCCAGGGTGCAGAGCCTCGGGCCGGCCTTGCTCCCCAAGAAGGGCTGACTGGGGCT >>>>>>>>> 240 CTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTG >>>>>>>>> ********************************************** 300 AGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCC ******************************************** 360 TCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGC <<<<<<<<<<<<<<<<<< 420 TGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAG 480 TCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAG 540 ATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAG 600 TCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3343 0 289 0 0 24 0 488 1840 0 0 0 24 0 678 Right 3758 0 245 0 0 1 0 356 2464 0 0 3 22 0 667 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR CETP_X16 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 62.38 65.00 2.00 0.00 9.00 CTCCTACTGCCCCTCCCTTC POSITION PENALTY, QUALITY: 0.000000, 2.381034 RIGHT PRIMER 394 18 60.61 66.67 6.00 2.00 8.00 CCCGACCTCCTTGGAGAC POSITION PENALTY, QUALITY: 0.000000, 2.609852 SEQUENCE SIZE: 672 INCLUDED REGION SIZE: 672 PRODUCT SIZE: 117, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,79 0 GAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTC 60 ATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGAT 120 GTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATT 180 CCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCC 240 CTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAG >>>>>>>>>>>>>>>>>>>>** 300 GGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTC ************************************************************ 360 CAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC *****************<<<<<<<<<<<<<<<<<< 420 ACCAGGCTCACAGCTGGAACCCTGGTGTCTCCTCCAGCGTGGTGGAAGTTGGGTTAGGAG 480 TACGGAGATGGAGATTGGCTCCCAACTCCTCCCTATCCTAAAGGCCCACTGGCATTAAAG 540 TGCTGTATCCAAGAGCTGCGGAGTCCTTCTTCTGTGGCTGGCGGGTAGAGGGGGGGGGAA 600 GGGATTGTCTCACCAGTGCCGTCCACCTCTTTTCAGCCCTTCCAAGCAGCTGCCCCCAAA 660 CCCTCCAAGCTT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3689 0 286 0 0 3 0 288 2492 0 0 0 22 0 598 Right 3637 0 289 0 0 3 0 299 2316 0 0 0 25 0 705 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X1 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 280 18 59.14 66.67 4.00 1.00 11.00 CCAGAGGGGTCAGGACAG POSITION PENALTY, QUALITY: 0.000000, 2.861995 RIGHT PRIMER 430 18 61.13 61.11 6.00 1.00 11.00 AGCCTGCAAGCACTCCCT POSITION PENALTY, QUALITY: 20.000000, 23.125223 SEQUENCE SIZE: 710 INCLUDED REGION SIZE: 710 PRODUCT SIZE: 151, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 298,114 0 AAGCAACCACCGCTATATGTTAGGATTCGAAGGAGCTCCAGGAAAGTCTCATAGCCCCAC 60 TGGCCAGAATGGGCTAAATCTCAGAGGGGGAGGGTGGGAGATGGGGGTGACAGTGACCTT 120 TTTTGTGACTCCTCCTAGACCATCCATCCCTGCTCCCAGGAGGACCTGTCCTCCCAGATG 180 GTGGAGATGGACAGGAGGACTATCTACCCACCCGTCCCCACGGCCCTGACCCTCTGACCT 240 CACCCTCTCCGCTGATTTCTTCATGTTAGTTCAACATTACCCAGAGGGGTCAGGACAGAC >>>>>>>>>>>>>>>>>>** 300 AATTCCTCAGTGACCCAGGAGCTGACACACTATGGCGCACGTCCGAGGCTTGCAGCTGCC ************************************************************ 360 TGGCTGCCTGGCCCTGGCTGCCCTGTGTAGCCTTGTGCACAGCCAGCATGGTAAGGGAGT **************************************************** <<<<<<< 420 GCTTGCAGGCTGGAACAGGCTGGAGGACTGGGGTGTGGGCCCATGGGCTGGGGTCTCCTG <<<<<<<<<<< 480 GCTGGACAGAGCACACAGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTA 540 GGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAG 600 GGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCC 660 TTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4041 0 289 0 0 21 0 498 2476 0 0 0 22 0 735 Right 4194 0 289 0 0 8 0 235 3082 0 0 2 0 0 578 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X2 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 265 18 60.05 61.11 4.00 0.00 9.00 GTCGCTGTTCCATGACCC POSITION PENALTY, QUALITY: 300.000000, 302.054062 RIGHT PRIMER 516 19 61.43 63.16 6.00 2.00 7.00 GTCTAGAGTTGGGCCCGGT POSITION PENALTY, QUALITY: 700.000000, 702.432556 SEQUENCE SIZE: 761 INCLUDED REGION SIZE: 761 PRODUCT SIZE: 252, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 298,165 0 AGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAG 60 CTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGC 120 AAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACC 180 AGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGGAGGGTGGGCTTGCTTCATGCCCCCAG 240 ATGGCCAAGACTGCCTGTTCCTGAGGTCGCTGTTCCATGACCCCCCCACCGCCTTTACAG >>>>>>>>>>>>>>>>>> ** 300 TGTTCCTGGCTCCTCAGCAAGCACGGTCGCTGCTCCAGCGGGTCCGGCGAGCCAACACCT ************************************************************ 360 TCTTGGAGGAGGTGCGCAAGGGCAACCTAGAGCGAGAGTGCGTGGAGGAGACGTGCAGCT ************************************************************ 420 ACGAGGAGGCCTTCGAGGCTCTGGAGTCCTCCACGGCTACGGTGAGCCTGGGCTGCTCGG ******************************************* 480 ACGGTGCCGGGGCCTCAGACCGGGCCCAACTCTAGACACTTCCACAGAGAAGCAAGCGAG <<<<<<<<<<<<<<<<<<< 540 GAACGCCACAGCCCCTTCGCTGCTCACAGCCTCATTTCAACTCTGAGCCCCTCCTCACAG 600 GGCTGGCAAGAGGAGCGGCCTCAGCCTTTCCTGGGGGTCTCTGTGCCTGGACTGTGTCCC 660 TGTGCAGCTCCATGACATGGGGAGGCCTCCACAGTCTTCAGACATCCACCTGCCTTGGAG 720 CTCTGTGTCCACATGGCCTCCTCAGCGGCAGACTCCCACAC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4542 0 289 0 0 14 0 245 3332 0 0 0 23 0 639 Right 4704 0 289 0 0 96 0 191 3583 0 4 0 0 0 541 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X3 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 60.48 50.00 2.00 0.00 11.00 ACTCCCAGCCCTTGTTTTTC POSITION PENALTY, QUALITY: 0.000000, 0.475706 RIGHT PRIMER 344 18 60.01 55.56 4.00 2.00 8.00 AAATCCTTCCCGGTGCTC POSITION PENALTY, QUALITY: 0.000000, 2.010701 SEQUENCE SIZE: 625 INCLUDED REGION SIZE: 625 PRODUCT SIZE: 67, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,29 0 TGTTCCTGGAGCTCTGTGTCGCCTTTCCTGTCTGTAGGGACTCTGCCAGGGACCCACTGC 60 CCCCTCTCCTCCCATCTCCCCCAGCCTCTTTCAGACTCGGTGTGTGTGTTGGAGGAACTC 120 CCCTATCCTCAAATATTCTTCTCCTTTTGGAAACAAAAGTAGGAAACTCTGCCACAAACC 180 TCCCCAGAGCCTGCCCCCTGCGTGACCAGGGTAAGGAAAGTGTGAGGAGGAGCATAACAT 240 TTACTAAAACAACACAAAACAGGAGCTGCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAG >>>>>>>>>>>>>>>>>>>>** 300 GATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAG ***************************<<<<<<<<<<<<<<<<<< 360 GCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCC 420 ACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCT 480 GTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCC 540 AAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGC 600 TTGCTGCATGTCTGGAAGGTGAGCA KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3344 0 289 0 0 7 0 659 1679 0 0 0 0 0 710 Right 3344 0 289 0 0 1 0 379 2040 0 0 0 0 0 635 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X4 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 271 21 60.63 52.38 4.00 2.00 8.00 CCAAAGGTAAACACCTGGGTC POSITION PENALTY, QUALITY: 120.000000, 121.626330 RIGHT PRIMER 372 20 61.69 55.00 4.00 0.00 8.00 CAAACCCGTGTCAGTTGCTC POSITION PENALTY, QUALITY: 0.000000, 1.693157 SEQUENCE SIZE: 651 INCLUDED REGION SIZE: 651 PRODUCT SIZE: 102, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,55 0 GCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGT 60 GAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCT 120 ACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTC 180 CTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCT 240 GACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAG >>>>>>>>>>>>>>>>>>>>> ** 300 CTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCAAC *****************************************************<<<<<<< 360 TGACACGGGTTTGGGGAGCAGGACATGGAGGGGAGCTTGGGAGAAGAGCTCAGGGGTGGG <<<<<<<<<<<<< 420 TTTGGAGTGTGGCTGGTGGAGGCCGAGGCAGTCCCCAGCATCTGACATTGCTCCCATTCC 480 TGGGGTCAAGATGTCTCTTTGTACCTGGCTCTGTGTCTGGCATGCGAACGAATGAATGAA 540 TGAATGGACTAATGAATTAATGTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAG 600 GCTGGAGTGCAGTGGCACGATCTTGGCTCACTGTAAACTCCGCCTCCCGGA KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3604 0 289 0 0 1 0 409 2209 3 14 0 0 0 679 Right 3497 0 289 0 0 107 0 371 2132 0 0 0 18 0 580 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X5 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 264 18 61.97 66.67 5.00 1.00 9.00 CAGGCTCCAAGGCTGACC POSITION PENALTY, QUALITY: 320.000000, 323.965248 RIGHT PRIMER 425 18 62.59 66.67 4.00 0.00 8.00 AAGGCCGACCCCTCACTC POSITION PENALTY, QUALITY: 0.000000, 4.589237 SEQUENCE SIZE: 706 INCLUDED REGION SIZE: 706 PRODUCT SIZE: 162, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,110 0 TGCTGGCCAAGAGAAGCCACAGAATGGAAGCTCCATGAGGGCAGGGCTGTGACTGTCCTA 60 TTGGTTGATGTGTACTGAGCACCCGACAGTGCCTGTCATATGGTAGGCACTTAGCGAATA 120 TTTGGAGGCCACTGTTGAGTGAATGGGAGAACTGCTGGTTGCAGAGGAAGAGGGGCTGGG 180 TGAATGCAGGTTCAGGATTGTGGACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTT 240 GCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAG >>>>>>>>>>>>>>>>>> ** 300 GTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAG ************************************************************ 360 GCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTC ************************************************<<<<<<<<<<<< 420 GGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCT <<<<<< 480 GTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGG 540 AGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACC 600 CCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGC 660 GACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4154 0 289 0 0 2 0 468 2494 0 0 0 0 0 901 Right 4154 0 289 0 0 14 0 310 2916 0 0 0 0 0 625 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X6 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 60.81 50.00 6.00 0.00 11.00 GTTGGGCAATTTCCTCTTCC POSITION PENALTY, QUALITY: 0.000000, 0.806671 RIGHT PRIMER 515 18 61.28 61.11 3.00 3.00 9.00 CTTGGTTCCCATCCCAGG POSITION PENALTY, QUALITY: 1180.000000, 1183.280619 SEQUENCE SIZE: 737 INCLUDED REGION SIZE: 737 PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 298,141 0 GACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTC 60 CCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGG 120 TACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAG 180 GAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAG 240 AACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAG >>>>>>>>>>>>>>>>>>>>** 300 AATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGA ************************************************************ 360 CAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATG ************************************************************ 420 CAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGG ******************* 480 CTTCATGGGGCCTGGCAGCCTGGGATGGGAACCAAGAATACTGGCTACCCAGGCACAGTG <<<<<<<<<<<<<<<<<< 540 GCTCATGCCCGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAGGTCAG 600 GGGTTTGAGACCAGCTGGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAAT 660 TGCCAGGCGTGGTGGTGGGCGCCTGTAATCCCAACTACTCTGGAGGCTGAGGCACGAGAA 720 TCGCTTGAACCCGGGAG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4464 0 289 0 0 2 0 392 2999 0 0 0 0 0 782 Right 4464 0 289 0 0 40 0 325 3245 0 0 0 0 0 565 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X7 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 269 18 62.25 66.67 3.00 1.00 11.00 CTCTGCTGCCTCCTTGCC POSITION PENALTY, QUALITY: 220.000000, 224.254433 RIGHT PRIMER 641 18 62.95 72.22 4.00 1.00 8.00 CTCAGGCCCCCTACCCAG POSITION PENALTY, QUALITY: 140.000000, 144.945754 SEQUENCE SIZE: 915 INCLUDED REGION SIZE: 915 PRODUCT SIZE: 373, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,319 0 GCAGCTCCAGGCAGCCACTACCAGTTGGTTACAGATGGCCTAGGAGGCCAAACCTGGTTA 60 CTATCTCTGGTTTATTATGTGCCAGACACTTATGCTGTATATTTTGTTTAATCCTCTCAA 120 CAAACCTGCAAAAGTGGCATTAGTAACCCCTTTAAAGGCAAACGGTCAGAAGCCCAGAGA 180 GGTTAAGTAACCTGAGGTCACACAGGCAGAAAGCAGCAAGACCGGGGTTCACACCCCTGT 240 CTGTTCCGGTCCATGTGTGGTCTCACTCACTCTGCTGCCTCCTTGCCCCTCACCCACCAG >>>>>>>>>>>>>>>>>> ** 300 GCCAGGATCAAGTCACTGTAGCGATGACTCCACGCTCCGAAGGCTCCAGTGTGAATCTGT ************************************************************ 360 CACCTCCATTGGAGCAGTGTGTCCCTGATCGGGGGCAGCAGTACCAGGGGCGCCTGGCGG ************************************************************ 420 TGACCACACATGGGCTCCCCTGCCTGGCCTGGGCCAGCGCACAGGCCAAGGCCCTGAGCA ************************************************************ 480 AGCACCAGGACTTCAACTCAGCTGTGCAGCTGGTGGAGAACTTCTGCCGCAACCCAGACG ************************************************************ 540 GGGATGAGGAGGGCGTGTGGTGCTATGTGGCCGGGAAGCCTGGCGACTTTGGGTACTGCG ************************************************************ 600 ACCTCAACTATTGTGGTGAGCTGCCTGGGTAGGGGGCCTGAGTTGCAGGGACAAATCCTA ***************** <<<<<<<<<<<<<<<<<< 660 GTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATT 720 ACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACA 780 CTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGC 840 ACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAAT 900 GGCCCAGCCCAGTCC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 6244 0 289 0 0 60 0 973 3795 0 0 0 0 0 1127 Right 5938 0 289 0 0 51 0 580 3983 0 0 0 44 0 991 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X8 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 246 18 61.28 61.11 2.00 2.00 9.00 GGATGGGTGAGGAATGGC POSITION PENALTY, QUALITY: 680.000000, 683.278099 RIGHT PRIMER 450 19 62.38 63.16 3.00 0.00 8.00 CCTCGGATGCCCACTACCT POSITION PENALTY, QUALITY: 20.000000, 23.380210 SEQUENCE SIZE: 729 INCLUDED REGION SIZE: 729 PRODUCT SIZE: 205, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 298,133 0 GAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTT 60 ACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTG 120 CCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGT 180 GCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGA 240 TCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAG >>>>>>>>>>>>>>>>>> ** 300 AGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCG ************************************************************ 360 AAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGG ************************************************************ 420 GAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAG *********** <<<<<<<<<<<<<<<<<<< 480 GACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGA 540 AGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCA 600 TTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCG 660 CTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGC 720 AGGCCTGGG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4078 0 289 0 0 87 0 484 2399 0 1 0 44 0 774 Right 4384 0 289 0 0 97 0 244 3201 0 0 0 0 0 553 Pair Stats: considered 14, high end compl 12, ok 2 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X9 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 279 19 61.85 57.89 2.00 2.00 9.00 ACTGCTTGGCTTGCTCTGC POSITION PENALTY, QUALITY: 0.000000, 2.845950 RIGHT PRIMER 454 18 61.32 61.11 5.00 3.00 8.00 AATGGTAGCGCAGGGCTC POSITION PENALTY, QUALITY: 160.000000, 163.321659 SEQUENCE SIZE: 727 INCLUDED REGION SIZE: 727 PRODUCT SIZE: 176, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 298,131 0 CCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAG 60 TCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGC 120 TGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTT 180 TCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGG 240 ATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAG >>>>>>>>>>>>>>>>>>>** 300 ACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGC ************************************************************ 360 TCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGT ************************************************************ 420 CACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCT ********* <<<<<<<<<<<<<<<<<< 480 GGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGGCTTTACAGATGACAACAGCTGAGC 540 ATCCAGGATCCCACCAACTCCACACAGCAGCCACATGAGATGGGTTGTTTACTTCTTTTT 600 TTTTTGTTTCTTAGATGGAGTCTTGCTCTGTCACCTAGGCTGGAGTGCAGTGCTGCAATC 660 TCGGCTCACTACCTCGATCTCAGCTCACTGCAACTTCTGCCTTCCGGGTTCAAACGATTC 720 TCTTGCC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4212 0 289 0 0 182 0 233 2939 0 0 0 22 0 547 Right 4248 0 289 0 0 80 0 415 2693 0 0 0 23 0 748 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X10 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 274 19 60.89 57.89 3.00 0.00 8.00 CTAATGCTTCCTGCTGCCC POSITION PENALTY, QUALITY: 100.000000, 101.889081 RIGHT PRIMER 487 18 61.58 61.11 4.00 2.00 10.00 ACGGGCCACCAGTTCTGT POSITION PENALTY, QUALITY: 0.000000, 3.583075 SEQUENCE SIZE: 768 INCLUDED REGION SIZE: 768 PRODUCT SIZE: 214, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,172 0 CCTGAGCCCAAGAGGCTGAGGCTTCAGTAAGCTGTGACTGTACCATTGCACTCCAGCCTG 60 GGTGACAAGAGTGAGACCCTGTCTCAAAAATAAATAAATAAATAAATAAAAAGTGTGAGG 120 CAGCCCCTCAGCATCACACGGAGGCTCCAGCCCCAAAGGCGGCCAGCCCAAGCTTGGATC 180 TGGGCCCCGGAGGCAGCTCTGCCCAGCTGGGTTCTTAGACCTGGGATTGTTACTTCTAGG 240 GCTGGTGTAGAGGCAGCCCCCTCATCCTCAGCTCCTAATGCTTCCTGCTGCCCCTCCCAG >>>>>>>>>>>>>>>>>>> ** 300 GCAGGTGATGCTTTTCCGGAAGAGTCCCCAGGAGCTGCTGTGTGGGGCCAGCCTCATCAG ************************************************************ 360 TGACCGCTGGGTCCTCACCGCCGCCCACTGCCTCCTGTACCCGCCCTGGGACAAGAACTT ************************************************************ 420 CACCGAGAATGACCTTCTGGTGCGCATTGGCAAGCACTCCCGCACAAGGTACAGAACTGG **************************************************<<<<<<<<<< 480 TGGCCCGTGGGTGTCTGGCAGGGGTCTGAGTCCTCCAAAGCGATCATGAGGGGCCTTGGT <<<<<<<< 540 GGCTCCGGGACACATAGGATGTTCTGTATACCCCCCAGAATATAACATCCCAGCAGTCTC 600 TGCTGGAAAGCCATTTGGTCACGTCCTGACTGAGGCTTGGAGCGCGGGGAGAATCCGTCT 660 GTCTCTGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCT 720 GTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4774 0 289 0 0 210 0 485 3153 0 0 0 0 0 637 Right 4621 0 289 0 0 8 0 474 3110 0 0 0 22 0 718 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X11 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 59.41 50.00 2.00 2.00 9.00 TTCTTTCTTGGGGTCTCTGC POSITION PENALTY, QUALITY: 0.000000, 0.594713 RIGHT PRIMER 493 18 62.11 61.11 4.00 3.00 9.00 ACAAGCATCTGGTGGCCC POSITION PENALTY, QUALITY: 0.000000, 4.108476 SEQUENCE SIZE: 774 INCLUDED REGION SIZE: 774 PRODUCT SIZE: 216, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,178 0 TGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTG 60 GACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGCTGTCTGACTCCAAAGCC 120 CTGCACGGCTTTAGGCCCAGGAAGAAACACCCAGGGGGCTGCCATGGCAGGAACCAGCCC 180 TATCCCCTCCCTGGTGGCCTGCAGGACACACTGTCTCCCAGAACCCCAAGGGCAGGCAGT 240 TTCCTGCTCCTTGCTGGGTGAACCTGCAGCTTCTCCATTTCTTTCTTGGGGTCTCTGCAG >>>>>>>>>>>>>>>>>>>>** 300 GTACGAGCGAAACATTGAAAAGATATCCATGTTGGAAAAGATCTACATCCACCCCAGGTA ************************************************************ 360 CAACTGGCGGGAGAACCTGGACCGGGACATTGCCCTGATGAAGCTGAAGAAGCCTGTTGC ************************************************************ 420 CTTCAGTGACTACATTCACCCTGTGTGTCTGCCCGACAGGGAGACGGCAGCCAGGTGGGC ********************************************************<<<< 480 CACCAGATGCTTGTTAGCTGAGGGGCAGAAGCCAAGTTCTGGGCCTGGCTCTGATACCAA <<<<<<<<<<<<<< 540 GTAGCCTTGCAAGAGCCCCTTTCCCTTTTCCAGGCCTCGGTTTCTTGGAGTGAACCCAAA 600 AGTTCTTTTCAGTACTGGCGTTTTATTTTTTATTTATATTTATTTATTTACTGACGGAGT 660 TCCACTCTTGTCTCCCAGGCTGGAGTGTAGTTGTGCGATCTTGGCTCACTGCAACCCCAC 720 CTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACA KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4834 0 289 0 0 0 0 620 3010 0 0 0 0 0 915 Right 4762 0 289 0 0 176 0 739 2528 0 0 0 13 0 1017 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X12 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 280 18 59.97 55.56 2.00 0.00 9.00 CCCTTCTTCCTTCCCCAA POSITION PENALTY, QUALITY: 0.000000, 2.027407 RIGHT PRIMER 515 18 62.87 66.67 3.00 2.00 8.00 CACTGTTCCCTCAGCCCG POSITION PENALTY, QUALITY: 280.000000, 284.867800 SEQUENCE SIZE: 782 INCLUDED REGION SIZE: 782 PRODUCT SIZE: 236, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,186 0 CCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACAGG 60 CTAATTTTTGTATTTTTAGTAGAGACTGGTGGGTTTCACCGTGTCGGCCAGGTTGGTCTC 120 GAACCCCTGACCTCAAGTGATTCACCCGCCTCGGCCTCCCAAAGTGCCGAGACCACAGGC 180 GTGAACGTCTGTGCCCAGCCAGCTCTGGCGTTTTAGATTCTGGTCTCTAAGAAATGGCGT 240 TGGGGCCAGGCGGCTCCTGTGGGGGTTGGCTCTCACTAGGCCCTTCTTCCTTCCCCAAAG >>>>>>>>>>>>>>>>>>** 300 CTTGCTCCAGGCTGGATACAAGGGGCGGGTGACAGGCTGGGGCAACCTGAAGGAGACGTG ************************************************************ 360 GACAGCCAACGTTGGTAAGGGGCAGCCCAGTGTCCTGCAGGTGGTGAACCTGCCCATTGT ************************************************************ 420 GGAGCGGCCGGTCTGCAAGGACTCCACCCGGATCCGCATCACTGACAACATGTTCTGTGC ************************************************************ 480 TGGCAAGTCTGTGCAGGGCGGGCTGAGGGAACAGTGGGGCCCAAGCTGGGAGAACTGAGT **** <<<<<<<<<<<<<<<<<< 540 TGTGCCTGGGTTCAAGCCATGTGACTTTGAGCAAGTTGCCTAACCTCTTGGTGGCTCAGT 600 TTCTTCCTCTGTAAAATGGAGGTAAAAGTCTCTATCCCATAAGGTTATGGGAGGGTTAAA 660 TGAAGTAGTATATATTAATGTACTTGGCATAGTATCAGTCACCAGTGAGCTCAGATAGCA 720 GCAAGAGGCTGCGGGTAGGGAAATGCCATTCATTCAGTCACTCAGCAAATATTTATTGAG 780 CG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4914 0 289 0 0 56 0 524 3341 0 0 0 0 0 704 Right 4914 0 289 0 0 83 0 976 2660 0 0 0 0 0 906 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X13 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 59.16 50.00 6.00 1.00 9.00 CAGACGGCTGTTCTCTTTCA POSITION PENALTY, QUALITY: 0.000000, 0.838952 RIGHT PRIMER 394 19 59.02 57.89 7.00 2.00 9.00 GCCCTGGGCTTTAGAGAAG POSITION PENALTY, QUALITY: 60.000000, 61.983042 SEQUENCE SIZE: 671 INCLUDED REGION SIZE: 671 PRODUCT SIZE: 117, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 298,75 0 AACTTTTTAAAAGCATTATTCTGCCTGTTGGGTGGAGAATAGACTGTAGGTGGGCAAAGA 60 ATGAAGGAAACTAGTGGGTTCAGGAGCTCGAGCTAGAAGTGGTGAGAAGGGTTTGGATTT 120 GGGGTCTATGCTGAAGGTAGAGCCGACAAGATTTGCTAGGATTGGATGTGTAGGGTGAGG 180 AAGTGGGGACAGCAAGAATGACTGGAGGGGTAAGTGGACTCTCACCAGCTGTGTCTCGTG 240 AAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAG >>>>>>>>>>>>>>>>>>>>** 300 GTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCT ************************************************************ 360 TTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTG ************* <<<<<<<<<<<<<<<<<<< 420 GGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGA 480 ACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTA 540 TCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTA 600 CACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTA 660 GGGGGCCACTC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3804 0 289 0 0 12 0 652 1841 0 0 0 0 0 1010 Right 3804 0 289 0 0 0 0 873 1715 0 5 0 0 0 922 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2_X14 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 274 24 60.11 37.50 5.00 0.00 10.00 TTGGAAACCTCATCTTTCTTCTTC POSITION PENALTY, QUALITY: 0.000000, 4.108177 RIGHT PRIMER 466 20 59.66 55.00 4.00 2.00 9.00 GAGCCCAGAATATGAGTGGC POSITION PENALTY, QUALITY: 20.000000, 20.341522 SEQUENCE SIZE: 685 INCLUDED REGION SIZE: 685 PRODUCT SIZE: 193, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,148 0 ACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGC 60 ACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTG 120 TGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCT 180 GGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCA 240 GAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAG >>>>>>>>>>>>>>>>>>>>>>>>** 300 AGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGAC ************************************************************ 360 CGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAG ************************************************************ 420 GTCATTGATCAGTTTGGAGAGTAGGGGGCCACTCATATTCTGGGCTCCTGGAACCAATCC ************************** <<<<<<<<<<<<<<<<<<<< 480 CGTGAAAGAATTATTTTTGTGTTTCTAAAACTATGGTTCCCAATAAAAGTGACTCTCAGC 540 GAGCCTCAATGCTCCCAGTGCTATTCATGGGCAGCTCTCTGGGCTCAGGAAGAGCCAGTA 600 ATACTACTGGATAAAGAAGACTTAAGAATCCACCACCTGGTGCACGCTGGTAGTCCGAGC 660 ACTCGGGAGGCTGAGGTGGGAGGAT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4475 0 230 0 0 0 0 988 2162 0 0 0 0 0 1095 Right 3944 0 289 0 0 74 0 898 1848 0 2 0 0 0 833 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2R_X1 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 168 18 62.11 66.67 2.00 1.00 9.00 GAGGGTGAAGCGGAGCAG POSITION PENALTY, QUALITY: 980.000000, 984.110493 RIGHT PRIMER 285 18 62.42 61.11 5.00 1.00 8.00 GCCGCACAGACTGAAGCA POSITION PENALTY, QUALITY: 57000.000000, 57004.415904 SEQUENCE SIZE: 329 INCLUDED REGION SIZE: 329 PRODUCT SIZE: 118, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 235,90 0 CTTCCTCGCTCGCGCCCGCGCGACCGCGCGCCCCAGTCCCGCCCCGCCCCGCTAACCGCC 60 CCAGACACAGCGCTCGCCGAGGGTCGCTTGGACCCTGATCTTACCCGTGGGCACCCTGCG 120 CTCTGCCTGCCGCGAAGACCGGCTCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCG >>>>>>>>>>>> 180 GAGCAGCCCGAGGCGGGGCAGCCTCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGG >>>>>> ***** 240 GCCGCGGCGGCTGCTGCTGGTGGCCGCCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGC ****************************((((((((((((((((((************** 300 CCGCACCCGGGCCCGCAGGCCAGGTGAGA ************************* KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 2940 0 0 0 0 829 0 31 1984 0 0 0 0 0 96 Right 951 0 226 0 0 209 0 0 510 0 0 0 0 0 6 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2R_X2-seg1 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 23 58.00 26.09 5.00 2.00 11.00 TTTCAGAATCAAAAGCAACAAAT POSITION PENALTY, QUALITY: 19000.000000, 19005.004751 RIGHT PRIMER 705 20 60.26 50.00 5.00 3.00 11.00 AGCAGGGTTTCATTGAGCAC POSITION PENALTY, QUALITY: 20000.000000, 20000.262854 SEQUENCE SIZE: 706 INCLUDED REGION SIZE: 706 PRODUCT SIZE: 706, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 4,702 0 TTTCAGAATCAAAAGCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACC >>>>)))))))))))))))))))************************************* 60 CCAATGATAAATATGAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTG ************************************************************ 120 AATACAGATTAGTCTCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCA ************************************************************ 180 TCTCAGAAGATGCCTCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTG ************************************************************ 240 TGTACACCGGAGTGTTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCA ************************************************************ 300 TCCTGAAAATGAAGGTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGGCCACGGCAG ************************************************************ 360 ATGTGCTGTTTGTGTCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATT ************************************************************ 420 GGCAGTTTGGGTCTGAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGTAACATGTACG ************************************************************ 480 CCTCTATCTTGCTCATGACAGTCATAAGCATTGACCGGTTTCTGGCTGTGGTGTATCCCA ************************************************************ 540 TGCAGTCCCTCTCCTGGCGTACTCTGGGAAGGGCTTCCTTCACTTGTCTGGCCATCTGGG ************************************************************ 600 CTTTGGCCATCGCAGGGGTAGTGCCTCTCGTCCTCAAGGAGCAAACCATCCAGGTGCCCG ************************************************************ 660 GGCTCAACATCACTACCTGTCATGATGTGCTCAATGAAACCCTGCT **************************(((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 6710 0 0 0 0 4 0 1539 3376 0 0 1 0 0 1790 Right 6710 0 0 0 0 4 0 1540 3376 0 0 2 0 0 1788 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F2R_X2-seg2 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 20 59.50 45.00 5.00 1.00 9.00 TGATGTGCTCAATGAAACCC POSITION PENALTY, QUALITY: 20000.000000, 20000.498267 RIGHT PRIMER 532 19 59.79 57.89 2.00 0.00 9.00 CTCCCAGCAGTCCCTTTTC POSITION PENALTY, QUALITY: 0.000000, 1.212099 SEQUENCE SIZE: 750 INCLUDED REGION SIZE: 750 PRODUCT SIZE: 533, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 0,514 0 TGATGTGCTCAATGAAACCCTGCTCGAAGGCTACTATGCCTACTACTTCTCAGCCTTCTC ))))))))))))))))))))**************************************** 60 TGCTGTCTTCTTTTTTGTGCCGCTGATCATTTCCACGGTCTGTTATGTGTCTATCATTCG ************************************************************ 120 ATGTCTTAGCTCTTCCGCAGTTGCCAACCGCAGCAAGAAGTCCCGGGCTTTGTTCCTGTC ************************************************************ 180 AGCTGCTGTTTTCTGCATCTTCATCATTTGCTTCGGACCCACAAACGTCCTCCTGATTGC ************************************************************ 240 GCATTACTCATTCCTTTCTCACACTTCCACCACAGAGGCTGCCTACTTTGCCTACCTCCT ************************************************************ 300 CTGTGTCTGTGTCAGCAGCATAAGCTCGTGCATCGACCCCCTAATTTACTATTACGCTTC ************************************************************ 360 CTCTGAGTGCCAGAGGTACGTCTACAGTATCTTATGCTGCAAAGAAAGTTCCGATCCCAG ************************************************************ 420 CAGTTATAACAGCAGTGGGCAGTTGATGGCAAGTAAAATGGATACCTGCTCTAGTAACCT ************************************************************ 480 GAATAACAGCATATACAAAAAGCTGTTAACTTAGGAAAAGGGACTGCTGGGAGGTTAAAA **********************************<<<<<<<<<<<<<<<<<<< 540 AGAAAAGTTTATAAAAGTGAATAACCTGAGGATTCTATTAGTCCCCACCCAAACTTTATT 600 GATTCACCTCCTAAAACAACAGATGTACGACTTGCATACCTGCTTTTTATGGGAGCTGTC 660 AAGCATGTATTTTTGTCAATTACCAGAAAGATAACAGGACGAGATGACGGTGTTATTCCA 720 AGGGAATATTGCCAATGCTACAGTAATAAA KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4999 0 227 0 0 0 0 1458 1982 0 0 0 22 0 1310 Right 6997 0 0 0 0 109 0 2483 2360 0 0 0 22 0 2023 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F3_X1 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 280 18 62.92 66.67 4.00 2.00 8.00 CTCGCCGCCAACTGGTAG POSITION PENALTY, QUALITY: 0.000000, 4.915955 RIGHT PRIMER 459 18 61.41 66.67 3.00 1.00 8.00 ACTGTCGCCTCCCTCCTG POSITION PENALTY, QUALITY: 800.000000, 803.413051 SEQUENCE SIZE: 700 INCLUDED REGION SIZE: 700 PRODUCT SIZE: 180, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 298,104 0 ACCGGGAGGAGGCGGGGCAGGGGTGTGGACTCGCCGGGGGCCGCCCACCGCGACGGCAAG 60 TGACCCGGGCCGGGGGCGGGGAGTCGGGAGGAGCGGCGGGGGCGGGCGCCGGGGGCGGGC 120 AGAGGCGCGGGAGAGCGCGCCGCCGGCCCTTTATAGCGCGCGGGGCACCGGCTCCCCAAG 180 ACTGCGAGCTCCCCGCACCCCCTCGCACTCCCTCTGGCCGGCCCAGGGCGCCTTCAGCCC 240 AACCTCCCCAGCCCCACGGGCGCCACGGAACCCGCTCGATCTCGCCGCCAACTGGTAGAC >>>>>>>>>>>>>>>>>>** 300 ATGGAGACCCCTGCCTGGCCCCGGGTCCCGCGCCCCGAGACCGCCGTCGCTCGGACGCTC ************************************************************ 360 CTGCTCGGCTGGGTCTTCGCCCAGGTGGCCGGCGCTTCAGGTGAGTGGCACCAGCCCCTG ****************************************** 420 GAAGCCCGGGGCGCGCCACACGCAGGAGGGAGGCGACAGTCCTGGCTGGCAGCGGGCTCG <<<<<<<<<<<<<<<<<< 480 CCCTGGTTCCCCGGGGCGCCCATGTTGTCCCCCGCGCCTACGGGACTCGGCTGCGCTCAC 540 CCAGCCCGGCTTGAATGAACCGAGTCCGTCGGGCGCCGGCGGGAGTTGCAGGGAGGGAGT 600 TGGCGCCCCAGACCCCGCTGCCCCTTCCGCTGGAGAGTTTTGCTCGGGGTGTCCGAGTAA 660 TTGGACTGTTGTTGCATAAGCGGACTTTTAGCTCCCGCTT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4094 0 289 0 0 1473 0 23 2263 0 0 0 0 0 46 Right 4094 0 289 0 0 716 0 101 2783 0 0 0 0 0 205 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F3_X2 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 266 27 57.78 33.33 6.00 0.00 11.00 CATGTAATAATAGCTGTTTCTCTGTTG POSITION PENALTY, QUALITY: 100.000000, 109.223369 RIGHT PRIMER 436 23 58.56 34.78 4.00 2.00 11.00 TTTTCTTTTTCTGTACCCAGCTT POSITION PENALTY, QUALITY: 0.000000, 4.440107 SEQUENCE SIZE: 712 INCLUDED REGION SIZE: 712 PRODUCT SIZE: 171, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,116 0 CTCTGGGCTCTTCCCGGTCAAGTTATAACAAGGCTTTGCCCATGAATAATTTCAAACGAA 60 AATGTCAAGATCCTTGCCGGTGTCCTGGGATTACAAGGTGAATCTTGTCATGAAGAAATT 120 CTAGGTCTAGAAAAAATTTGAAGATTCTTTTTCTCTTGATAATTCACTAATGAAGCTTTT 180 GTGGTTGAAAAATAAAAAGTGAGGTTTATGGTGATGTCAGGTGGGAAGGTGTTTTATACA 240 TCAATACATTCGAGTGCTCTGAAGTGCATGTAATAATAGCTGTTTCTCTGTTGTTTAAAG >>>>>>>>>>>>>>>>>>>>>>>>>>> ** 300 GCACTACAAATACTGTGGCAGCATATAATTTAACTTGGAAATCAACTAATTTCAAGACAA ************************************************************ 360 TTTTGGAGTGGGAACCCAAACCCGTCAATCAAGTCTACACTGTTCAAATAAGGTAAGCTG ******************************************************<<<<<< 420 GGTACAGAAAAAGAAAATTAAGGTCTTTGATGTTTCTACTGTCCTATGCTGAACAAGAAT <<<<<<<<<<<<<<<<< 480 GTCTTTAAAGCTGATTACTGGATGAAATTATTTAACAGATGACGAAGAAGAAGGGATTCT 540 TGGCAATTCGCTGGCCGGTGTCATACTCTATTAGGCCTGCAACATTTCCAGACCTTAAAC 600 TGATAGAACATTTTAATTGTTTTAATTGTTTTTGGAAATGATGGGAGAGTTCCTAAGTGG 660 AGTATAAACTGTGGAGAGATGAACCATCTTGAGTAGGCACTGAAGTGTGCTT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4082 0 289 0 0 83 0 1878 779 0 0 1 20 0 1032 Right 4214 0 289 0 0 232 0 1968 682 0 0 0 0 0 1043 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F3_X3 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 264 24 57.53 29.17 4.00 0.00 11.00 TTCCATTTATGTTTCTTCCTTCTT POSITION PENALTY, QUALITY: 200.000000, 206.472471 RIGHT PRIMER 521 20 57.40 45.00 3.00 3.00 9.00 TATTACAGCCCAAGCCACTT POSITION PENALTY, QUALITY: 0.000000, 2.604792 SEQUENCE SIZE: 800 INCLUDED REGION SIZE: 800 PRODUCT SIZE: 258, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,204 0 TGAGGACTCAAACATGCATCCCAAGTGCTCGGTGTTGCTAGGTATGATGGCTCACACCTG 60 TACATTCAGCACTTTGGGAGGCCGAAGCAGAAGGATCAGCCTGGGCAACATAGCAGGACC 120 CCATCTCTACAAAACAATGTTTAAAAAAAAGCAAAGTGCTCAGCACAGTGACTGCATCAT 180 TAGGATTGATTGTAGGGCTCCTGATGTTAGCACAGAACACCACAGCCAGGAAGCAGTCTA 240 TCTTGTTGGGTGCAAATTGTAACATTCCATTTATGTTTCTTCCTTCTTTTCTTTCTTTAG >>>>>>>>>>>>>>>>>>>>>>>> ** 300 CACTAAGTCAGGAGATTGGAAAAGCAAATGCTTTTACACAACAGACACAGAGTGTGACCT ************************************************************ 360 CACCGACGAGATTGTGAAGGATGTGAAGCAGACGTACTTGGCACGGGTCTTCTCCTACCC ************************************************************ 420 GGCAGGGAATGTGGAGAGCACCGGTTCTGCTGGGGAGCCTCTGTATGAGAACTCCCCAGA ************************************************************ 480 GTTCACACCTTACCTGGAGAGTAAGTGGCTTGGGCTGTAATACCGTTCATTCTTGTTAGA **********************<<<<<<<<<<<<<<<<<<<< 540 AACGTCTGAACATTCTCGTGATCTTGTGCCTTTAGGGGCTACAAAATTAAAAATATTTAT 600 TCTTTTTTTCTCAGAAACTGGTATGTATCACAGCCCTCTTCACACATTCCAGATGTGGTA 660 GGAGGTTCACAGAATGTGAACTTTTGGAGCTGATGACAGTGTCATCAAGTAACTTTCTCC 720 CCCAGTCTGTCCCCAGACCCTGTTACTGTCCTCAGTAAGCGGCTGAATGTGTGTTGGGAG 780 AGGGCGGGCCAGGGAAGCGG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4965 0 289 0 0 54 0 1212 2106 0 0 28 22 0 1254 Right 5005 0 289 0 0 185 0 1231 1945 0 0 0 11 0 1344 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F3_X4 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 276 22 60.11 40.91 4.00 0.00 11.00 CCAAGCTCATTTCCTTTGTTTC POSITION PENALTY, QUALITY: 0.000000, 2.108929 RIGHT PRIMER 523 27 58.95 25.93 6.00 1.00 10.00 TCACAATTTAAAACAGGTCATAAAAAC POSITION PENALTY, QUALITY: 320.000000, 328.049552 SEQUENCE SIZE: 779 INCLUDED REGION SIZE: 779 PRODUCT SIZE: 248, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 298,183 0 TGGTTAAATAAAATATATTATTAAAATTAATTTCACCTGTTCCTTTTTACTTTTTCTAAT 60 GTGACTACTAGAAAACTTAAAATGACATCTGAGGCTCCATTGTCTTCCCCTTGGGCCAGC 120 ACTACCACAGAATGTCTTAGGATTCAGCTCCAGGCCGCCACGCCTGCTTCTTTCAGGGAG 180 CTGGTTCTATGCACATGTTTTATATGAGAGATAATTAAGTTGTCAATTGTGATAACAAAA 240 CAGGATTTGACTTTGTACAGAATTCTTTGGTTCCAACCAAGCTCATTTCCTTTGTTTCAG >>>>>>>>>>>>>>>>>>>>>>** 300 CAAACCTCGGACAGCCAACAATTCAGAGTTTTGAACAGGTGGGAACAAAAGTGAATGTGA ************************************************************ 360 CCGTAGAAGATGAACGGACTTTAGTCAGAAGGAACAACACTTTCCTAAGCCTCCGGGATG ************************************************************ 420 TTTTTGGCAAGGACTTAATTTATACACTTTATTATTGGAAATCTTCAAGTTCAGGAAAGG ************************************************************ 480 TGAGCATTTTTTAATTTGTTTTTATGACCTGTTTTAAATTGTGAATACTTGGTTTTACAA * <<<<<<<<<<<<<<<<<<<<<<<<<<< 540 CCCATTTCTTCCCCAATTCAAAAATAGCAGAACAGAGTTGTTGAGAAGGTGATGGAGTAG 600 AAGGGGGAGCGCGCACTGTGGGGAGGGGTGGACAACAGGCCTGGTCCTACCTGTGACTCT 660 GCACTACCCTGTGACTCTGGCAGGGCCCCCTCGGAGACCCAGGTTCCTCAGCCAACCGGC 720 TGGATCAGGTCATCTCTAAAGGTCCCGCCACGCTCACATTTCTCCCTCTATTGAGGATC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4884 0 289 0 0 320 0 1911 1322 0 6 0 0 0 1036 Right 4770 0 289 0 0 176 0 1330 1913 0 0 0 21 0 1041 Pair Stats: considered 36, high end compl 33, ok 3 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F3_X5 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 268 27 57.07 29.63 7.00 1.00 10.00 CCAGATGAATTAAGAACTCTATCTTTT POSITION PENALTY, QUALITY: 60.000000, 69.931898 RIGHT PRIMER 485 18 62.91 61.11 5.00 1.00 9.00 GGCAAATGGCTGGCAGAG POSITION PENALTY, QUALITY: 120.000000, 124.906952 SEQUENCE SIZE: 760 INCLUDED REGION SIZE: 760 PRODUCT SIZE: 218, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,164 0 CAAAATTTGTTTTTGGTTCAATGCATAATACTCCCTTCCTTTTTCTTTTACTGCAGATAT 60 CTTCTAAAGGGGCTCAATAGGGTTCAATATGCCTAAATTGGATCTTCTCAGTCTTGGAAA 120 AGGCATTTTTAGCAGTGATCAAGGGAAACTGATTAGCGAAGTCACTTCTAATCCTTCACG 180 TGTCAGCTGTGTTCTTGTAGGCTTTGCTTAGAACCTAGGTTTTTACTTCCACAGTGACTT 240 AATAAAGGGGAAAGAATTGACTCAGAGCCCAGATGAATTAAGAACTCTATCTTTTTACAG >>>>>>>>>>>>>>>>>>>>>>>>>>> ** 300 AAAACAGCCAAAACAAACACTAATGAGTTTTTGATTGATGTGGATAAAGGAGAAAACTAC ************************************************************ 360 TGTTTCAGTGTTCAAGCAGTGATTCCCTCCCGAACAGTTAACCGGAAGAGTACAGACAGC ************************************************************ 420 CCGGTAGAGTGTATGGGCCAGGAGAAAGGGGAATTCAGAGGTGAGTGGCTCTGCCAGCCA ****************************************** <<<<<<<<<<<< 480 TTTGCCTGGGGGTATGGGTGCTGTGGGTGACTTCTGGAGGAGTAGCTCCACCCTCAGGGC <<<<<< 540 TGGGATATACTTCCTTGGTTAAATATTCAGGAAAACAAACTGCCTGGAGGTTTTTTGTTG 600 TTATTTGTTTGTTTTGGTTTTGATTTTGCTTTGGTACAAAAAAGATTTTGGACATTTAGA 660 AATGTTTCTGTGTTGATTGTGCCCTTGTATTAGCAGGTGTTTTCTTGAGCACCTGTCATG 720 TGCTAAGCCCTCTGCTGAGCACTGGATACACAAACTGTGT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4694 0 289 0 0 4 0 2035 844 0 0 0 0 0 1522 Right 4397 0 289 0 0 28 0 1323 1565 0 4 0 44 0 1144 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F3_X6 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 274 24 58.01 33.33 4.00 0.00 11.00 TTGACTAGATGCCCTTTATTTTTC POSITION PENALTY, QUALITY: 0.000000, 5.991812 RIGHT PRIMER 459 21 57.77 52.38 4.00 1.00 11.00 CAGTAGCTCCAACAGTGCTTC POSITION PENALTY, QUALITY: 0.000000, 3.226865 SEQUENCE SIZE: 737 INCLUDED REGION SIZE: 737 PRODUCT SIZE: 186, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,141 0 CAGCATCGTTTTAGGCACTTCTTTCCAGCCATTGTTCTTACCTCCAACTACATATCTTTT 60 CTGGACTGTGCATTATTCAGTTTATTAAATGCCCATTAAATGTGTTTAGCCATTGTCAAT 120 TACTCTGAAACGTTCAGGTTTTGACAAATTCTTTCCTAATGTAAGTGTGGTGGAAAGAGT 180 GAAAGAAAGTCAAATTGCACAAAAATAGGATGGTGTAATTTGGGGTTATGCCGTCAATTT 240 TGTCCACTGATAAATGGGATTTGAGCTCTCCAAGTTGACTAGATGCCCTTTATTTTTCAG >>>>>>>>>>>>>>>>>>>>>>>>** 300 AAATATTCTACATCATTGGAGCTGTGGTATTTGTGGTCATCATCCTTGTCATCATCCTGG ************************************************************ 360 CTATATCTCTACACAAGTGTAGAAAGGCAGGAGTGGGGCAGAGCTGGAAGGAGAACTCCC ************************************************************ 420 CACTGAATGTTTCATAAAGGAAGCACTGTTGGAGCTACTGCAAATGCTATATTGCACTGT *******************<<<<<<<<<<<<<<<<<<<<< 480 GACCGAGAACTTTTAAGAGGATAGAATACATGGAAACGCAAATGAGTATTTCGGAGCATG 540 AAGACCCTGGAGTTCAAAAAACTCTTGATATGACCTGTTATTACCATTAGCATTCTGGTT 600 TTGACATCAGCATTAGTCACTTTGAAATGTAACGAATGGTACTACAACCAATTCCAAGTT 660 TTAATTTTTAACACCATGGCACCTTTTGCACATAACATGCTTTAGATTATATATTCCGCA 720 CTCAAGGAGTAACCAGG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4464 0 289 0 0 57 0 1831 878 0 0 0 0 0 1409 Right 4311 0 289 0 0 51 0 1766 895 0 0 0 22 0 1288 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F5_X10 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 23 23 59.52 21.74 6.00 3.00 10.00 TTGCAAATGAAAACAATTTTGAA POSITION PENALTY, QUALITY: 260.000000, 263.479042 RIGHT PRIMER 302 24 59.24 33.33 5.00 0.00 10.00 TGAAAGGTTACTTCAAGGACAAAA POSITION PENALTY, QUALITY: 20.000000, 24.758462 SEQUENCE SIZE: 319 INCLUDED REGION SIZE: 319 PRODUCT SIZE: 280, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 59,219 0 TTATTTATTATCATGAAATAACTTTGCAAATGAAAACAATTTTGAATATATTTTCTTTCA >>>>>>>>>>>>>>>>>>>>>>> * 60 GGCAGGAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTATAAGTGG ************************************************************ 120 AACATCTTAGAGTTTGATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATAC ************************************************************ 180 TACAGTGACGTGGACATCATGAGAGACATCGCCTCTGGGCTAATAGGACTACTTCTAATC ************************************************************ 240 TGTAAGAGCAGATCCCTGGACAGGCGAGGAATACAGGTATTTTGTCCTTGAAGTAACCTT ************************************** <<<<<<<<<<<<<<<<<<<<< 300 TCAGAAATTCTGAGAATTT <<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 2597 0 32 0 0 195 0 1010 658 0 23 8 0 0 671 Right 2435 0 50 0 0 0 0 1068 632 0 0 0 0 0 685 Pair Stats: considered 18, high end compl 16, ok 2 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F7_X1 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 60.30 50.00 6.00 2.00 8.00 GCAGCACTGCAGAGATTTCA POSITION PENALTY, QUALITY: 0.000000, 0.295503 RIGHT PRIMER 385 18 59.44 61.11 4.00 2.00 7.00 GAGAAAACCTCCCCGGAC POSITION PENALTY, QUALITY: 40.000000, 42.564912 SEQUENCE SIZE: 664 INCLUDED REGION SIZE: 664 PRODUCT SIZE: 108, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,68 0 ATTTGAAGTGTTGGTGCCACACACACCTTAACACCTGCACGCTGGCAACAAAACCGTCCG 60 CTCTGCAGCACAGCTGGGGTCACCTGACCTTTCTCCTGTCCCCCCCACTTGAGCTCAGTG 120 GCTGGGCAGCAGGGGATGCATGGCCACTGGCGGCCAGGTGCAGCTCTCAGCTGGGGTGTT 180 CAGAGGACGCCTGTGTCCTCCCCTCCCCCATCCCTCTGTCACCCTTGGAGGCAGAGAACT 240 TTGCCCGTCAGTCCCATGGGGAATGTCAACAGGCAGGGGCAGCACTGCAGAGATTTCATC >>>>>>>>>>>>>>>>>>>>** 300 ATGGTCTCCCAGGCCCTCAGGCTCCTCTGCCTTCTGCTTGGGCTTCAGGGCTGCCTGGCT ************************************************************ 360 GCAGGTGCGTCCGGGGAGGTTTTCTCCATAAACTTGGTGGAAGGGCAGTGGGCAAATCCA ****** <<<<<<<<<<<<<<<<<< 420 GGAGCCAGCCCGGGCTTCCCAAACCCCGCCCTTGCTCCGGACACCCCCATCCACCAGGAG 480 GGTTTTCTGGCGGCTCCTGTTCAATTTCTTTCCTTCTAGAAACCAGCATCCAGGCACAGG 540 AGGGGAGGCCCTTCTTGGTAGCCCAGGCTTTGGCGGGATTATTTTTCAAAGAACTTTAGG 600 AGTGGGTGGTGCTTTCCTGGCCCCCATGGCCCTGCCTGTGAGGTCGGACAAGCGCAGGGA 660 GTCT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3572 0 289 0 0 0 0 118 2723 0 0 0 23 0 419 Right 3734 0 289 0 0 17 0 289 2735 0 1 0 0 0 403 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F7_X2 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 265 18 62.24 61.11 4.00 2.00 8.00 CATGGCCTTATGACCCCG POSITION PENALTY, QUALITY: 300.000000, 304.241567 RIGHT PRIMER 385 18 60.58 66.67 5.00 3.00 9.00 CAGTGGCAGTCCCTGCTC POSITION PENALTY, QUALITY: 0.000000, 2.576172 SEQUENCE SIZE: 666 INCLUDED REGION SIZE: 666 PRODUCT SIZE: 121, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,70 0 TGTGTGGGGTGTGGGGATAGGGTCAGGGGATGGCGTGGGGGGTGTGGCATGGGGATGGCA 60 CGTGTGGCATGGGGATGGGGATGGGGGGTGGGGCATGGCCGAGTGGGGCTGGGGCTGGGA 120 ATGGTGAGTGGGGCATGGGGATGGCGAGTAGGGGGTGTGGCGTGAGGATGGCTAGTGGGG 180 CGTGGGGATGGCGTGTGGGGATGGCGAGTGGGGGGTGGGCTGTGAGGGACAGTGCCTGGG 240 ATGTGGCTGCAGCCCTAGCTCACAGCATGGCCTTATGACCCCGGCCACCTTCCTGCCCAG >>>>>>>>>>>>>>>>>> ** 300 GCGGGGTCGCTAAGGCCTCAGGAGGAGAAACACGGGACATGCCGTGGAAGCCGGGGCCTC ************************************************************ 360 ACAGAGGTGAGCAGGGACTGCCACTGGTTTTGTCCTGGGGCCCAGTGGGGGCAACATCAC ********<<<<<<<<<<<<<<<<<< 420 CTCCTTCCCCTCCCATGGCAAAGAGCCAGCCCGCGGGGTGGCTACTGCAGTGCCCCCCAA 480 GGAGGGTGTTCCCTGCTCGAGAGGAAGTGACCGCTCCAGCTTGGCCTTCCCTGGGACTGG 540 GGTGCAGGCGATTTTATCTTCTTTGCTCCATTCTGTTCCTTCCAGATAATCGTGTGTTCT 600 TCATCAGGTTTTCCTCAGTTCTTGAGAGCTTTTCTGATGCAAATCTGCTTTCACCCCAGG 660 GCGGTC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3301 0 289 0 0 28 0 14 2710 0 1 0 65 0 194 Right 3601 0 289 0 0 16 0 390 2278 0 0 0 22 0 606 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F7_X2' Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 276 19 58.86 47.37 4.00 2.00 9.00 CTTCACGGAACTCGCATTT POSITION PENALTY, QUALITY: 60.000000, 62.138031 RIGHT PRIMER 546 18 61.66 61.11 3.00 2.00 9.00 AGCCAAAGAGACGCAGGC POSITION PENALTY, QUALITY: 1320.000000, 1323.664208 SEQUENCE SIZE: 761 INCLUDED REGION SIZE: 761 PRODUCT SIZE: 271, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,165 0 AGCCTCACGTTTACCGCGGCGCCCGCAGCCCCCTTCGCCCGCTTCCGCGCGTGCCCCCGA 60 GCGCGCCCTCGGGATCAGCCCCCGGAAGCAGAGAGGCCAGGCCGGGAAGGATGGGCGAAC 120 GGGGTGGCTGACCCGGGAGCACGGCAGGGAGGACACCCAGCCAGGCCCGCGAGCAGCGCC 180 GCTCCCCTCCTCCAGGACGGGCGGGAACCTGCGATGCCCCCGCCGCGTGGGCCGTGGGGC 240 GGTCTCCGAGGCACTGGGCGGGGCACGCGGTGGGCGCTTCACGGAACTCGCATTTCCCAG >>>>>>>>>>>>>>>>>>> ** 300 TCTTCGTAACCCAGGAGGAAGCCCACGGCGTCCTGCACCGGCGCCGGCGCGCCAACGCGT ************************************************************ 360 TCCTGGAGGAGCTGCGGCCGGGCTCCCTGGAGAGGGAGTGCAAGGAGGAGCAGTGCTCCT ************************************************************ 420 TCGAGGAGGCCCGGGAGATCTTCAAGGACGCGGAGAGGACGGTGAGCCCAGCCTCGGGGC ******************************************* 480 GCCCCGCGCGGACACTGCACGGCGGCGGTGAACCAGGCCGCGTGGGGCCGCCTGCGTCTC <<<<<<<<<<< 540 TTTGGCTGCGGCCTGTGGGCGGCGAACACGCAGCGGCGCCCGCGCGCGCGCTCTCTCTGC <<<<<<< 600 GGGGGTCGCTTTCCGCCCGGGGTGACTCCGCTTTCCTGGGCGATGCCCCCACCCCCAGGC 660 ACGCGCTCTCCCCGTGCGGCCGCACCGCGCATGCCGGTTTTCACATCAGAAAATACGATT 720 TGCACAAGCACACTTAGGGTGTCCCCCTTAACTTCCCAAGG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4704 0 289 0 0 1097 0 35 3097 0 6 0 0 0 180 Right 4704 0 289 0 0 1078 0 138 2874 0 0 0 0 0 325 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F7_X3 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 277 21 60.51 52.38 2.00 0.00 10.00 GGCTTGCTCTCTTGTTCCTTC POSITION PENALTY, QUALITY: 0.000000, 1.510814 RIGHT PRIMER 359 18 59.83 61.11 4.00 2.00 11.00 GTTGCAGGCAGGACTGGT POSITION PENALTY, QUALITY: 300.000000, 302.172769 SEQUENCE SIZE: 625 INCLUDED REGION SIZE: 625 PRODUCT SIZE: 83, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,29 0 CAGAGCCTGTGTACCTCGTCCCAGGTCCACAGCTCAGCGACAGAAGAGTCAGGGTTGAAC 60 CTCGGGTGTTCTGACTTGGGAGCAGGAAATGTGTGGTCACCCATAGTTCCAGATGTCCTG 120 GGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGA 180 GCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGC 240 TTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAG >>>>>>>>>>>>>>>>>>>>>** 300 AAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAAC *************************** <<<<<<<<<<<<<<<<<< 360 CCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCC 420 ATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCC 480 TGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCA 540 GAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACC 600 TGGTGGGGTGTGTAGGCCGGGCATT KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3344 0 289 0 0 2 0 424 1910 0 0 0 0 0 719 Right 3344 0 289 0 0 0 0 284 2312 8 13 7 0 0 431 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F7_X4 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 280 18 60.10 66.67 2.00 0.00 10.00 GACACCAGCCCACTCCAC POSITION PENALTY, QUALITY: 0.000000, 2.096539 RIGHT PRIMER 433 18 62.23 61.11 5.00 1.00 8.00 GACCCAAAGTGGGGCCTT POSITION PENALTY, QUALITY: 0.000000, 4.227266 SEQUENCE SIZE: 714 INCLUDED REGION SIZE: 714 PRODUCT SIZE: 154, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,118 0 GTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGC 60 TCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAA 120 CCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCAC 180 GAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGAT 240 GATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAG >>>>>>>>>>>>>>>>>>** 300 ATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCC ************************************************************ 360 AGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGG ********************************************************<<<< 420 CCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCC <<<<<<<<<<<<<< 480 AACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATTCAGGGCTCAG 540 CCCAGTTGGAAATTGGTCTAGGTGACCTTTAAATCCCTTCCAGTCTGAGGTCTTTGACAG 600 GGACCCAAGGTTCTGATTATCAGACTCAGTGGCCCCCTTCGCGGTCCCGGCCCTGGGCAA 660 CTTCTCAGCCCTGGAGACTGGCCCAGTTGAGAGTCCCTGTGTCCCGTGTGCCCA KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4234 0 289 0 0 2 0 537 2588 0 0 0 0 0 818 Right 4234 0 289 0 0 81 0 378 2891 0 0 0 0 0 595 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F7_X5 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 277 18 60.31 66.67 3.00 1.00 9.00 GGGTGTCCCCTTCCTGTC POSITION PENALTY, QUALITY: 60.000000, 62.311131 RIGHT PRIMER 461 18 60.05 55.56 4.00 1.00 11.00 TGGGACATGAAGCCTGGT POSITION PENALTY, QUALITY: 20.000000, 22.045801 SEQUENCE SIZE: 741 INCLUDED REGION SIZE: 741 PRODUCT SIZE: 185, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 298,145 0 GCTCGGAAGAGTGGTCACCCTGGAGGCCACTGCCCACCAGCCAGGCACCCCCCAAATGCA 60 ACCGCAGCCAGCACTGCCAGCCACTGGCAAGGCTGTTCAGACATGTGGCTCCTCTGATCC 120 ACGCCTTGTCCTTTGGATCAGTCCACGGAGCAGTGTGCCAAGCTCAGGCTCTGTCACCCA 180 CAGCTCATGCCACCTTCCAGGCAGAACACCACTGCTGACCCAGGGGCATGGCCACCCCGG 240 GGGCTGGCGTCTCGCTGACCCCCAGAAGCCCCTCTCAGGGTGTCCCCTTCCTGTCCCCAG >>>>>>>>>>>>>>>>>> ** 300 ACAAGGATGACCAGCTGATCTGTGTGAACGAGAACGGCGGCTGTGAGCAGTACTGCAGTG ************************************************************ 360 ACCACACGGGCACCAAGCGCTCCTGTCGGTGCCACGAGGGGTACTCTCTGCTGGCAGACG ************************************************************ 420 GGGTGTCCTGCACACCCACAGGTGACCAGGCTTCATGTCCCAGTCCCAGATGACACCAGT *********************** <<<<<<<<<<<<<<<<<< 480 CCCTGTCCCACTAGGATTATCTTACTGGACAAAAGACGGGTGGGACTGGCCTTCACATCT 540 ACTGAGCACTAACTATGCACTGACCAATTGTGAGGTGGGATCTGGGCACCAAGGGTGGCA 600 CAGGCCAGCAGCGACCAGTGACTAGGATGGGCACCCTGGGGGCAATCCCTGAATGGCCTC 660 AGGCCCCCTGCCAACTTCTAGGCAGACCAGGGGAGCCAAGCAAGGCACTATCTCACGTCC 720 AACTGCCCACTCGCAGGAATC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4351 0 289 0 0 139 0 152 3259 0 0 0 22 0 490 Right 4504 0 289 0 0 1 0 499 3030 0 0 2 0 0 683 Pair Stats: considered 20, high any compl 8, high end compl 10, ok 2 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F7_X6 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 272 26 59.68 34.62 3.00 3.00 9.00 GCATCTTTCTGACTTTTGTTTTACAC POSITION PENALTY, QUALITY: 0.000000, 6.324793 RIGHT PRIMER 434 19 61.51 57.89 4.00 0.00 8.00 ATCCTGAAGCCAGGGGAAG POSITION PENALTY, QUALITY: 80.000000, 82.512614 SEQUENCE SIZE: 710 INCLUDED REGION SIZE: 710 PRODUCT SIZE: 163, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,114 0 GAAAAGTGAGCAGAGGGCGGTTTTAAGACGTAAGCCCTCTGTTTCCTCCAAAACCAGCCC 60 TGACCATTGTCTCCTCAGCCAGCCACTTCTTCAAGGGCCTCTCATGGCCGGGCCCCACCA 120 GTCAGGCCCAGCCGAGGCCCTGCCTTCCACCACCCCTGGGCCCTGGGAGCTCCTGCTCCT 180 GGGGGCCTCCCATAGCCTCGGCCTCAAGGCCTCTCAGAGGATGGGTGTTTCTGAATCTTT 240 CCTAGTGGCACGTTCATCCCTCACAAATCTCTGCATCTTTCTGACTTTTGTTTTACACAG >>>>>>>>>>>>>>>>>>>>>>>>>>** 300 TTGAATATCCATGTGGAAAAATACCTATTCTAGAAAAAAGAAATGCCAGCAAACCCCAAG ************************************************************ 360 GCCGAATTGTGGGGGGCAAGGTGTGCCCCAAAGGGGAGTGTCCATGGCAGGTAAGGCTTC **************************************************** <<<< 420 CCCTGGCTTCAGGATTCCAAGCCCTGAGGGTCTTGAAGCCTTTTGAATGTGAACAACAGC <<<<<<<<<<<<<<< 480 TCTGGAAGGGAAAATGGGCAGGTCAGCCCAAGCCCACAGGCTCCAAGTCAGCACACCTAG 540 CACCTCCAGCTCGCGGCACCCCCATGCTTTTAGTGGGGCAAGGAAGGAGAAAAGAAAACG 600 ACACTCACTGAGGGTCTACCCTGTGCAGAGAACCCTGCGAGATGCCCCATCCGAGTTGTC 660 ACGTCGTCCTCACGGTTACTCTTTGAGGTGGGATCTTTGCCTGATCTTTG KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 3891 0 289 0 0 43 0 599 2265 0 0 0 44 0 651 Right 3894 0 289 0 0 11 0 463 2371 0 1 0 44 0 715 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F7_X7 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 262 21 59.64 47.62 4.00 0.00 9.00 AGCAATGTGACTTCCACACCT POSITION PENALTY, QUALITY: 300.000000, 301.362873 RIGHT PRIMER 447 19 61.08 63.16 2.00 2.00 9.00 CGGACAGGGGAGAGTGGTA POSITION PENALTY, QUALITY: 60.000000, 62.075168 SEQUENCE SIZE: 724 INCLUDED REGION SIZE: 724 PRODUCT SIZE: 186, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 298,128 0 CTTTGCAAAATCAGGAGCATTGGATCAAAGCTATGTGAAGATCCTGTGAGGTGAACAGTG 60 AAATCTCACAGCGACATTTGTATTCTTGGGCCGTGCCCAAGAGCACGTCTCGGCTAGAGA 120 GGGGCACAGCCTCCCAGAGCCAGGTCTGAGCAGCTTTGCCTGGGAGGGATCTGCAAAGAC 180 CCCAGGATTTCAGAAAGAAATTGTGCAATGCCAGAGGTTCCTTGGCATGCCCGGGAGGGC 240 GAGTCATCAGAGAAACAATGACAGCAATGTGACTTCCACACCTCCTGTCCCCCCGCCCAG >>>>>>>>>>>>>>>>>>>>> ** 300 GTCCTGTTGTTGGTGAATGGAGCTCAGTTGTGTGGGGGGACCCTGATCAACACCATCTGG ************************************************************ 360 GTGGTCTCCGCGGCCCACTGTTTCGACAAAATCAAGAACTGGAGGAACCTGATCGCGGTG ************************************************************ 420 CTGGGTGGGTACCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTTCCCT ****** <<<<<<<<<<<<<<<<<<< 480 GTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGG 540 TGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCGCTGTCCGACCG 600 CGGTGCTGGGTGGGTACCACTCTCCCCTGTCTGACCGCAGCTCTCAAGTGTCTCAGGGGC 660 TGTGGCTCTGGGCTTCGTGCTGTCACTTCCACAGACAGACAGACATCCCCAAAAGGGGAG 720 CAAC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4032 0 289 0 0 6 0 577 2278 0 0 0 44 0 838 Right 4181 0 289 0 0 10 0 236 3155 0 5 0 22 0 464 Pair Stats: considered 126, high any compl 28, high end compl 97, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR F7_X8 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 265 18 62.95 66.67 5.00 3.00 10.00 GTGGAAAGGGCCTGAGGG POSITION PENALTY, QUALITY: 300.000000, 304.949476 RIGHT PRIMER 922 18 59.90 61.11 4.00 3.00 8.00 TTCTCTCCACAGGCCAGG POSITION PENALTY, QUALITY: 140.000000, 142.096979 SEQUENCE SIZE: 1196 INCLUDED REGION SIZE: 1196 PRODUCT SIZE: 658, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 298,600 0 GCCGGGAAGAACTGTGGAGAATCGGGGCACGCCCTGTCCTCCCAGCTGCCAGGGCACAGC 60 ATCCCTTCCCCACCTGCAACACCCAGACCCCAGATTCACCCCAGTTCACTTGTCCCCACA 120 CGAGCCACAGGCTGCCACCTGGGGCAGGCTGGCCCACCTTGGGGTTAGATGCAGGTCCCC 180 TTGCCCCAGAAGGAGACTGCAGCCCCTGCAGACCTAGAAATGGCCACAGCCCATCCCCAT 240 GCACCAGGGGGTGAGGTGGCAGGTGGTGGAAAGGGCCTGAGGGGGGCTTCTTCCTTCCAG >>>>>>>>>>>>>>>>>> ** 300 GCGAGCACGACCTCAGCGAGCACGACGGGGATGAGCAGAGCCGGCGGGTGGCGCAGGTCA ************************************************************ 360 TCATCCCCAGCACGTACGTCCCGGGCACCACCAACCACGACATCGCGCTGCTCCGCCTGC ************************************************************ 420 ACCAGCCCGTGGTCCTCACTGACCATGTGGTGCCCCTCTGCCTGCCCGAACGGACGTTCT ************************************************************ 480 CTGAGAGGACGCTGGCCTTCGTGCGCTTCTCATTGGTCAGCGGCTGGGGCCAGCTGCTGG ************************************************************ 540 ACCGTGGCGCCACGGCCCTGGAGCTCATGGTCCTCAACGTGCCCCGGCTGATGACCCAGG ************************************************************ 600 ACTGCCTGCAGCAGTCACGGAAGGTGGGAGACTCCCCAAATATCACGGAGTACATGTTCT ************************************************************ 660 GTGCCGGCTACTCGGATGGCAGCAAGGACTCCTGCAAGGGGGACAGTGGAGGCCCACATG ************************************************************ 720 CCACCCACTACCGGGGCACGTGGTACCTGACGGGCATCGTCAGCTGGGGCCAGGGCTGCG ************************************************************ 780 CAACCGTGGGCCACTTTGGGGTGTACACCAGGGTCTCCCAGTACATCGAGTGGCTGCAAA ************************************************************ 840 AGCTCATGCGCTCAGAGCCACGCCCAGGAGTCCTCCTGCGAGCCCCATTTCCCTAGCCCA ********************************************************** 900 GCAGCCCTGGCCTGTGGAGAGAAAGCCAAGGCTGCGTCGAACTGTCCTGGCACCAAATCC <<<<<<<<<<<<<<<<<< 960 CATATATTCTTCTGCAGTTAATGGGGTAGAGGAGGGCATGGGAGGGAGGGAGAGGTGGGG 1020 AGGGAGACAGAGACAGAAACAGAGAGAGACAGAGACAGAGAGAGACTGAGGGAGAGACTC 1080 TGAGGACATGGAGAGAGACTCAAAGAGACTCCAAGATTCAAAGAGACTAATAGAGACACA 1140 GAGATGGAATAGAAAAGATGAGAGGCAGAGGCAGACAGGCGCTGGACAGAGGGGCA KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 8901 0 289 0 0 87 0 201 7523 0 0 0 22 0 779 Right 9054 0 289 0 0 79 0 1105 6374 0 1 0 0 0 1206 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HCF2_X2 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 59.94 50.00 1.00 0.00 8.00 TGTGTTCTGTTTTCCCTCCC POSITION PENALTY, QUALITY: 0.000000, 0.056936 RIGHT PRIMER 1231 23 60.71 47.83 4.00 2.00 9.00 TGTGAGAACTGTAAAGGTGCCTC POSITION PENALTY, QUALITY: 40.000000, 43.705322 SEQUENCE SIZE: 1505 INCLUDED REGION SIZE: 1505 PRODUCT SIZE: 954, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 298,909 0 CTCCTCCCTGGGATGCCTGAGCTCCCAGGATTCTAGAAGGTTAGTTTTGCAAACCTTTAA 60 AGAAGGGATTTTCATCAAGGGGCCCACAGATCCTTCATTGAGGTTTATGAGTCCCACATC 120 AAAGGTTGGGTGTCTATCTACATCAGATTCTCTTAAAGTCCATGATCCTAAAACAGTTAA 180 GAACTAATGCTGTGAGGGCCTCTTCCTGGGTCAAAGCCACAGGGAACCTGCCATGTGGAT 240 GCTGCAGCGGGGTGTGGATCAGCCAGGCCGCCTTTCACTGTGTTCTGTTTTCCCTCCCAG >>>>>>>>>>>>>>>>>>>>** 300 CTTTAGCTCCGCCAAAATGAAACACTCATTAAACGCACTTCTCATTTTCCTCATCATAAC ************************************************************ 360 ATCTGCGTGGGGTGGGAGCAAAGGCCCGCTGGATCAGCTAGAGAAAGGAGGGGAAACTGC ************************************************************ 420 TCAGTCTGCAGATCCCCAGTGGGAGCAGTTAAATAACAAAAACCTGAGCATGCCTCTTCT ************************************************************ 480 CCCTGCCGACTTCCACAAGGAAAACACCGTCACCAACGACTGGATTCCAGAGGGGGAGGA ************************************************************ 540 GGACGACGACTATCTGGACCTGGAGAAGATATTCAGTGAAGACGACGACTACATCGACAT ************************************************************ 600 CGTCGACAGTCTGTCAGTTTCCCCGACAGACTCTGATGTGAGTGCTGGGAACATCCTCCA ************************************************************ 660 GCTTTTTCATGGCAAGAGCCGGATCCAGCGTCTTAACATCCTCAACGCCAAGTTCGCTTT ************************************************************ 720 CAACCTCTACCGAGTGCTGAAAGACCAGGTCAACACTTTCGATAACATCTTCATAGCACC ************************************************************ 780 CGTTGGCATTTCTACTGCGATGGGTATGATTTCCTTAGGTCTGAAGGGAGAGACCCATGA ************************************************************ 840 ACAAGTGCACTCGATTTTGCATTTTAAAGACTTTGTTAATGCCAGCAGCAAGTATGAAAT ************************************************************ 900 CACGACCATTCATAATCTCTTCCGTAAGCTGACTCATCGCCTCTTCAGGAGGAATTTTGG ************************************************************ 960 GTACACACTGCGGTCAGTCAATGACCTTTATATCCAGAAGCAGTTTCCAATCCTGCTTGA ************************************************************ 1020 CTTCAAAACTAAAGTAAGAGAGTATTACTTTGCTGAGGCCCAGATAGCTGACTTCTCAGA ************************************************************ 1080 CCCTGCCTTCATATCAAAAACCAACAACCACATCATGAAGCTCACCAAGGGCCTCATAAA ************************************************************ 1140 AGATGCTCTGGAGAATATAGACCCTGCTACCCAGATGATGATTCTCAACTGCATCTACTT ************************************************************ 1200 CAAAGGTAAGAGGCACCTTTACAGTTCTCACAGCAAACCCACAACATACTATTTTTGTAT ******* <<<<<<<<<<<<<<<<<<<<<<< 1260 GTGGGTAGATTGAATGCCAAGAACTGTACTGTAGCTATAATTTATCCAGGAAAACTAGAC 1320 ACAAGATTGACTCTGGAACGGGGACAGGGAAGGCCAAGCTGAAGTGACAGTAGCATCTGA 1380 CACTTACTGAGCCCTAACTCTGTGCTTTAACACAGCCTTGTGAGGTCATCACTGTTATTA 1440 GCATCCCCATTTTACAGAGGAAGCCACCAACACATGAAGTAAAAGGATGGGCTGGGCGCG 1500 GTGGC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 12144 0 289 0 0 12 0 3242 5342 0 0 0 0 0 3259 Right 12144 0 289 0 0 15 0 3651 4687 0 7 0 0 0 3495 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HCF2_X3 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 58.79 55.00 4.00 0.00 8.00 CCTCTTCCTGTGGCCTTTAC POSITION PENALTY, QUALITY: 0.000000, 1.210543 RIGHT PRIMER 605 23 58.48 39.13 3.00 3.00 10.00 GCTCAAAAGAACAAACACACAGT POSITION PENALTY, QUALITY: 140.000000, 144.517111 SEQUENCE SIZE: 874 INCLUDED REGION SIZE: 874 PRODUCT SIZE: 328, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 298,278 0 TTTGACAGATGTGACAGATGAAGAAATAGAAGCGAGTTAGGTGCCTTACCATGGTCAAAC 60 AACTAGTTCGTATCAGACCCTACTCCAGAAACTATTCCAGTCCGGGTAACCTCTCGTTAA 120 CCTCTCTTGTTAGAAATGCAAATTTCTGCCCAAATCAGGCCTCAGGAATCAAGAGACTGT 180 GGGGTCGGCTCTGCAGGCTATCTGAATGAGGCCTCCAGGGAAATCAGATTCACTCTCAAG 240 GGTGAGACGATTTCCCTAAAGGAACCTTCTCATAACAGCCTCTTCCTGTGGCCTTTACAG >>>>>>>>>>>>>>>>>>>>** 300 GATCCTGGGTGAATAAATTCCCAGTGGAAATGACACACAACCACAACTTCCGGCTGAATG ************************************************************ 360 AGAGAGAGGTAGTTAAGGTTTCCATGATGCAGACCAAGGGGAACTTCCTCGCAGCAAATG ************************************************************ 420 ACCAGGAGCTGGACTGCGACATCCTCCAGCTGGAATACGTGGGGGGCATCAGCATGCTAA ************************************************************ 480 TTGTGGTCCCACACAAGATGTCTGGGATGAAGACCCTCGAAGCGCAACTGACACCCCGGG ************************************************************ 540 TGGTGGAGAGATGGCAAAAAAGCATGACAAACAGGTATTTCACACTGTGTGTTTGTTCTT ************************************ <<<<<<<<<<<<<<<<< 600 TTGAGCTCCCAGATGCTGGGGGTGTCTGGGAATACTGGAAAATGGATCATTTTTTTAAAA <<<<<< 660 AGGGAGAATTATGTACAAGTACCCAAGAACTTCCATACAGGGCCACTCTGTTAATTCAGC 720 CCCAATTTGTTGCTTGAGATAAGAGATGATTAGAGAGCATTCATAAGGGACACATCTGCC 780 CTCTAGGGGCCAGTTTCAGAAGTTAGAGGCAGATGACTTAGAGACAGCTTGGTGCTTGCT 840 TTGTGGCTTCGAGTCCCAGCTTCATCATCCCTAA KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 5543 0 289 0 0 0 0 1266 2461 0 0 0 38 0 1489 Right 5393 0 289 0 0 28 0 1331 2335 19 7 0 67 0 1317 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HCF2_X4 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 278 20 59.55 50.00 1.00 0.00 9.00 CCCCCTTTCCTTTTCTGTCT POSITION PENALTY, QUALITY: 0.000000, 0.451656 RIGHT PRIMER 465 19 59.80 57.89 2.00 0.00 8.00 GGTGGACAAGGGAGTGGTT POSITION PENALTY, QUALITY: 0.000000, 1.198986 SEQUENCE SIZE: 745 INCLUDED REGION SIZE: 745 PRODUCT SIZE: 188, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,149 0 ATGCCTTTTTAAACCATAAACCAGGAAAAGTTCTGCTGCTAACCTTGAAGATAGGAAACG 60 AACCATACAGTCTCAAGGAAATAATCATGCAACAGAAAACACACCTCAGTTTTCAGTAGC 120 GGAATTACAAAGGAGTGTGCTTCCTAAAATCCTCAACTGACAGTCCCGGAATATAAATTT 180 TAATAAGTGCTATATCAATTCTGTGATAAATATAACCCGTGGCCCTTTAAAGGGAAAATC 240 ATGATTCTTTTGTAACTTGTGGTTCAATAAAACTGGGCCCCCCTTTCCTTTTCTGTCTAG >>>>>>>>>>>>>>>>>>>>** 300 AACTCGAGAAGTGCTTCTGCCGAAATTCAAGCTGGAGAAGAACTACAATCTAGTGGAGTC ************************************************************ 360 CCTGAAGTTGATGGGGATCAGGATGCTGTTTGACAAAAATGGCAACATGGCAGGCATCTC ************************************************************ 420 AGACCAAAGGATCGCCATCGACCTGGTAACCACTCCCTTGTCCACCCCCGACCCGTCCCC ***************************<<<<<<<<<<<<<<<<<<< 480 AGGGTCTGCCTCAGCACAGCCCCACCTCCACTTGCCCTTCCTACCCACCCCCCAATCTCA 540 TGTCCCAGCTTGGGGTGCTGAGTCTGCTCTTCGGCCTGGGTGGGATACACAGAATGCCTA 600 GTTTCATGGATGCCAGCTGGAGAGCACGGCACCTGGCAGACACTTACTGGGCAGGGGGGA 660 TCCCAAGAGCAGCCATGGGGTGAGCCCCACTCCCGCTGACACCAGAGACAGGGGAGACAT 720 GTGCTGCGGTCTGGGAAATAGCTAC KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 4391 0 289 0 0 128 0 1570 1096 0 0 0 22 0 1286 Right 4238 0 289 0 0 20 0 420 2770 0 0 0 44 0 695 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HCF2_X5 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 275 23 59.66 39.13 4.00 2.00 10.00 TCTGACAACTTTCCTTTCCAAAC POSITION PENALTY, QUALITY: 0.000000, 3.339047 RIGHT PRIMER 1193 23 59.76 34.78 4.00 2.00 10.00 TTGGCTTTTGATATTTGAGCTTC POSITION PENALTY, QUALITY: 460.000000, 463.239398 SEQUENCE SIZE: 1446 INCLUDED REGION SIZE: 1446 PRODUCT SIZE: 919, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 298,850 0 CCCGGAGAAGTGCGCAGCAGTGTGGGGAGCTGGAGCTGGGGTGGCTGTCCTGCACCAGCC 60 CCCACGACCCTCAGACCACAGGCACTGCCAAGAGGGAACATGAACCTAGCCGGCCTCTAA 120 GTGCAACGGCTGCCCCTGACAGGTGGTGACAGATATTTTCAAGAGTGACTCTGACCAGCT 180 GTGATTTCCACCTTACATGTTGTCTTTGGATCCTTTCCCTGAATGATATGAGATTGTGCT 240 GGGAACTCTAGCCCTCTGTGTGCTGACCTCCAGAATCTGACAACTTTCCTTTCCAAACAG >>>>>>>>>>>>>>>>>>>>>>>** 300 TTCAAGCACCAAGGCACGATCACAGTGAACGAGGAAGGCACCCAAGCCACCACTGTGACC ************************************************************ 360 ACGGTGGGGTTCATGCCGCTGTCCACCCAAGTCCGCTTCACTGTCGACCGCCCCTTTCTT ************************************************************ 420 TTCCTCATCTACGAGCATCGCACCAGCTGCCTGCTCTTCATGGGAAGAGTGGCCAACCCC ************************************************************ 480 AGCAGGTCCTAGAGGTGGAGGTCTAGGTGTCTGAAGTGCCTTGGGGGCACCCTCATTTTG ************************************************************ 540 TTTCCATTCCAACAACGAGAACAGAGATGTTCTGGCATCATTTACGTAGTTTACGCTACC ************************************************************ 600 AATCTGAATTCGAGGCCCATATGAGAGGAGCTTAGAAACGACCAAGAAGAGAGGCTTGTT ************************************************************ 660 GGAATCAATTCTGCACAATAGCCCATGCTGTAAGCTCATAGAAGTCACTGTAACTGTAGT ************************************************************ 720 GTGTCTGCTGTTACCTAGAGGGTCTCACCTCCCCACTCTTCACAGCAAACCTGAGCAGCG ************************************************************ 780 CGTCCTAAGCACCTCCCGCTCCGGTGACCCCATCCTTGCACACCTGACTCTGTCACTCAA ************************************************************ 840 GCCTTTCTCCACCAGGCCCCTCATCTGAATACCAAGCACAGAAATGAGTGGTGTGACTAA ************************************************************ 900 TTCCTTACCTCTCCCAAGGAGGGTACACAACTAGCACCATTCTTGATGTCCAGGGAAGAA ************************************************************ 960 GCCACCTCAAGACATATGAGGGGTGCCCTGGGCTAATGTTAGGGCTTAATTTTCTCAAAG ************************************************************ 1020 CCTGACCTTTCAAATCCATGATGAATGCCATCAGTCCCTCCTGCTGTTGCCTCCCTGTGA ************************************************************ 1080 CCTGGAGGACAGTGTGTGCCATGTCTCCCATACTAGAGATAAATAAATGTAGCCACATTT ************************************************************ 1140 ACTGTGTATCTGTTATAATTCTCTATTTTTTGAAGCTCAAATATCAAAAGCCAAATCCAA ******** <<<<<<<<<<<<<<<<<<<<<<< 1200 ATTCCTGGATAACTCCAGGTATGATAAAGGCTGAGAGGAAGTCACTTGAGCACCACAATG 1260 TGCCACAGCAGGGCATGTTCTCAGGACAGGACAGGTGTGTGCTGAATCCTGGGGAGGGTC 1320 TGTGCAGTACCCCAGAACTGTGGGGTGCTAAGTGGCACACAAGCCCCAGGGCTCCCACAG 1380 TCTATGCCAGGCTGCTGCAGCTTTCATCCCTCATACCTGGTCCTGCAGTGGGTCTGGTTT 1440 GACAGA KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 11554 0 289 0 0 7 0 2490 6121 0 0 0 0 0 2647 Right 11432 0 289 0 0 57 0 2518 5947 0 12 0 20 0 2589 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X1 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 39 20 57.72 50.00 6.00 3.00 8.00 TGAGATCTGGAGGATCCAAG POSITION PENALTY, QUALITY: 300.000000, 302.282278 RIGHT PRIMER 237 24 57.87 29.17 7.00 2.00 9.00 TCTTCAAACTTTGGACATTCATAA POSITION PENALTY, QUALITY: 24000.000000, 24006.125439 SEQUENCE SIZE: 238 INCLUDED REGION SIZE: 238 PRODUCT SIZE: 199, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 74,164 0 CCAGGCTGAGCGTCGGCGCCGGGGTTCGGTGGCCTCTAGTGAGATCTGGAGGATCCAAGG >>>>>>>>>>>>>>>>>>>> 60 ATTCTGTAGCTACAATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCC ********************************************** 120 ATCCCTGGGAAGTCATAGTGGGGACAGTGACACTGACCATCTGCATGATGTCCATGAACA ************************************************************ 180 TGTTTACTGGTAACAATAAGATCTGTGGTTGGAATTATGAATGTCCAAAGTTTGAAGA **********************************(((((((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 2030 0 0 0 0 67 0 611 842 3 38 0 0 0 469 Right 1490 0 65 0 0 0 0 478 584 0 0 0 0 0 363 Pair Stats: considered 11, high end compl 10, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X2 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 20 57.20 45.00 3.00 3.00 10.00 GGATGTTTTGAGCAGTGACA POSITION PENALTY, QUALITY: 20000.000000, 20002.797253 RIGHT PRIMER 108 26 57.38 26.92 8.00 2.00 9.00 AATATATTTTGATCCAAGTTGACGTA POSITION PENALTY, QUALITY: 29000.000000, 29008.623090 SEQUENCE SIZE: 112 INCLUDED REGION SIZE: 112 PRODUCT SIZE: 109, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 0,112 0 GGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGCATAGCCATCCTGTA ))))))))))))))))))))**************************************** 60 TATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTG ***********************((((((((((((((((((((((((((*** KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 770 0 0 0 0 5 0 460 81 0 0 0 0 0 224 Right 770 0 0 0 0 11 0 448 81 7 1 0 0 0 222 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X3 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 19 58.71 47.37 4.00 1.00 9.00 GGTATTGCTGGCCTTTTCA POSITION PENALTY, QUALITY: 19000.000000, 19002.290742 RIGHT PRIMER 87 21 57.47 38.10 4.00 0.00 9.00 TCAAGCCTGTCAATTCTTTGT POSITION PENALTY, QUALITY: 21000.000000, 21003.532432 SEQUENCE SIZE: 88 INCLUDED REGION SIZE: 88 PRODUCT SIZE: 88, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 0,88 0 GGTATTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCAC )))))))))))))))))))***************************************** 60 TTCTTAGACAAAGAATTGACAGGCTTGA *******((((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 530 0 0 0 0 1 0 361 44 0 0 0 0 0 124 Right 530 0 0 0 0 1 0 416 4 0 0 0 0 0 109 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X4 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 20 58.44 40.00 6.00 0.00 10.00 ATGAAGCTTTGCCCTTTTTC POSITION PENALTY, QUALITY: 20000.000000, 20001.557050 RIGHT PRIMER 84 19 57.08 52.63 6.00 0.00 8.00 TGTGAGTTGGAACTGAGGG POSITION PENALTY, QUALITY: 19000.000000, 19003.924962 SEQUENCE SIZE: 85 INCLUDED REGION SIZE: 85 PRODUCT SIZE: 85, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 0,85 0 ATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTAGCAA ))))))))))))))))))))**************************************** 60 AGTTTGCCCTCAGTTCCAACTCACA ******((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 500 0 0 0 0 0 0 151 175 0 0 0 0 0 174 Right 500 0 0 0 0 0 0 123 202 0 0 0 0 0 175 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X5 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 25 60.11 36.00 6.00 2.00 10.00 GGATGAAGTAAGGGAAAATATTGCT POSITION PENALTY, QUALITY: 25000.000000, 25005.106680 RIGHT PRIMER 105 22 59.72 45.45 8.00 0.00 11.00 TGACATGGTACCAACTCCAATC POSITION PENALTY, QUALITY: 22000.000000, 22002.282868 SEQUENCE SIZE: 106 INCLUDED REGION SIZE: 106 PRODUCT SIZE: 106, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 0,106 0 GGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTTTAGGTCCTACGTTTACCCT )))))))))))))))))))))))))*********************************** 60 CGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCA ************************(((((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 710 0 0 0 0 0 0 212 246 0 1 0 0 0 251 Right 710 0 0 0 0 0 0 202 248 0 1 1 0 0 258 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X6 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 18 57.14 55.56 4.00 3.00 10.00 GGGGTACGTCAGCTTGAA POSITION PENALTY, QUALITY: 18000.000000, 18004.862764 RIGHT PRIMER 106 23 58.33 43.48 3.00 2.00 11.00 TCTAATACCAAGGACACACAAGC POSITION PENALTY, QUALITY: 23000.000000, 23004.673725 SEQUENCE SIZE: 107 INCLUDED REGION SIZE: 107 PRODUCT SIZE: 107, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 0,107 0 GGGGTACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTAC ))))))))))))))))))****************************************** 60 TTCGTGTTCATGACTTTCTTCCCAGCTTGTGTGTCCTTGGTATTAGA ************************((((((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 720 0 0 0 0 0 0 206 309 0 0 0 0 0 205 Right 720 0 0 0 0 0 0 181 338 0 0 0 0 0 201 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X7 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 17 18 61.02 55.56 4.00 3.00 9.00 GCGAGGGTCGTCCAATTT POSITION PENALTY, QUALITY: 35000.000000, 35003.015981 RIGHT PRIMER 116 24 57.39 37.50 5.00 1.00 9.00 ATAATCATCTTGACCCTCTGAGTT POSITION PENALTY, QUALITY: 24000.000000, 24006.612999 SEQUENCE SIZE: 117 INCLUDED REGION SIZE: 117 PRODUCT SIZE: 100, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 0,117 0 GCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGCAGCTCAGCCATTTTGCCCGAGT *****************))))))))))))))))))************************* 60 TTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAAGATGATTAT *********************************(((((((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 820 0 0 0 0 3 0 162 464 0 0 0 0 0 191 Right 820 0 0 0 0 3 0 237 349 0 0 0 0 0 231 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X8 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 1 22 57.75 45.45 4.00 0.00 10.00 TCTCTAGGCTTGGTTCTTGTTC POSITION PENALTY, QUALITY: 23000.000000, 23004.249004 RIGHT PRIMER 154 22 59.14 45.45 5.00 0.00 11.00 GATAAAACTGCCAGAGGGAAAC POSITION PENALTY, QUALITY: 28000.000000, 28002.863756 SEQUENCE SIZE: 161 INCLUDED REGION SIZE: 161 PRODUCT SIZE: 154, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 0,161 0 GTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCA *))))))))))))))))))))))************************************* 60 AAACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAG ************************************************************ 120 AATTGAACCAAGTGTTTCCCTCTGGCAGTTTTATCTCTCTA *************((((((((((((((((((((((****** KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 1260 0 0 0 0 0 0 540 321 0 0 0 0 0 399 Right 1260 0 0 0 0 0 0 530 315 0 0 20 0 0 395 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X9 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 23 58.46 30.43 6.00 3.00 11.00 AAATGATCAGCATGGATATTGAA POSITION PENALTY, QUALITY: 23000.000000, 23004.538081 RIGHT PRIMER 247 21 57.37 38.10 3.00 2.00 10.00 TTTTCTTTCTCGGTTTATCCC POSITION PENALTY, QUALITY: 21000.000000, 21003.627403 SEQUENCE SIZE: 248 INCLUDED REGION SIZE: 248 PRODUCT SIZE: 248, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 0,248 0 AAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAGTTTAGCTCTCCTTCTGGCTG )))))))))))))))))))))))************************************* 60 TCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTAAAAAACCCTA ************************************************************ 120 TCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTA ************************************************************ 180 TGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAG ***********************************************((((((((((((( 240 AAAGAAAA (((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 1977 0 0 0 0 1 0 1154 176 0 1 0 22 0 623 Right 1977 0 0 0 0 0 0 1125 203 0 0 0 22 0 627 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X10 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 1 24 57.83 37.50 8.00 3.00 9.00 TTGAGGTTATAAAACCCTTAGTGG POSITION PENALTY, QUALITY: 25000.000000, 25006.169796 RIGHT PRIMER 89 24 57.71 41.67 4.00 0.00 9.00 TGAAGTATCGAGTAAGGAGGAGTT POSITION PENALTY, QUALITY: 24000.000000, 24006.288343 SEQUENCE SIZE: 90 INCLUDED REGION SIZE: 90 PRODUCT SIZE: 89, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 0,90 0 GTTGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTG *))))))))))))))))))))))))*********************************** 60 GTTGGTAACTCCTCCTTACTCGATACTTCA ******(((((((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 550 0 0 0 0 0 0 222 109 0 0 0 0 0 219 Right 550 0 0 0 0 0 0 227 116 0 0 0 0 0 207 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X11 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 1 22 59.54 54.55 8.00 3.00 10.00 CAGTACTGGTGACACAGGAACC POSITION PENALTY, QUALITY: 23000.000000, 23002.457721 RIGHT PRIMER 88 21 57.28 42.86 4.00 0.00 8.00 TCTGCATTCCCAAGTATCTGT POSITION PENALTY, QUALITY: 21000.000000, 21003.718624 SEQUENCE SIZE: 89 INCLUDED REGION SIZE: 89 PRODUCT SIZE: 88, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 0,89 0 TCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGCCTAATGAA *))))))))))))))))))))))************************************* 60 GAATGTCTACAGATACTTGGGAATGCAGA ********((((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 540 0 0 0 0 0 0 135 249 0 1 0 0 0 155 Right 540 0 0 0 0 0 0 181 215 0 0 0 0 0 144 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X12 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 23 59.21 39.13 4.00 1.00 9.00 GAAAGGTGCAAAATTCCTTAGTG POSITION PENALTY, QUALITY: 23000.000000, 23003.785045 RIGHT PRIMER 194 27 57.50 33.33 8.00 2.00 9.00 AAGGAGTAATTATAATCCCTGTAAGGT POSITION PENALTY, QUALITY: 27000.000000, 27009.501708 SEQUENCE SIZE: 195 INCLUDED REGION SIZE: 195 PRODUCT SIZE: 195, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 0,195 0 GAAAGGTGCAAAATTCCTTAGTGATGCTGAGATCATCCAGTTAGTCAATGCTAAGCATAT )))))))))))))))))))))))************************************* 60 CCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGTGGTGTATCTATTCGCCG ************************************************************ 120 ACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTTACAGGGA ************************************************(((((((((((( 180 TTATAATTACTCCTT ((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 1600 0 0 0 0 0 0 646 347 0 0 0 0 0 607 Right 1600 0 0 0 0 0 0 707 324 0 0 0 0 0 569 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X13 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 18 57.47 55.56 4.00 0.00 9.00 GGTGATGGGAGCTTGTTG POSITION PENALTY, QUALITY: 18000.000000, 18004.525596 RIGHT PRIMER 158 19 58.71 57.89 6.00 1.00 10.00 CCTATTGCTCTGCAGCCTC POSITION PENALTY, QUALITY: 19000.000000, 19002.288404 SEQUENCE SIZE: 159 INCLUDED REGION SIZE: 159 PRODUCT SIZE: 159, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 0,159 0 GGTGATGGGAGCTTGTTGTGAGAATGTTATTGGATATATGCCCATCCCTGTTGGAGTGGC ))))))))))))))))))****************************************** 60 AGGACCCCTTTGCTTAGATGAAAAAGAATTTCAGGTTCCAATGGCAACAACAGAAGGTTG ************************************************************ 120 TCTTGTGGCCAGCACCAATAGAGGCTGCAGAGCAATAGG ********************((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 1240 0 0 0 0 1 0 314 591 0 0 0 0 0 334 Right 1240 0 0 0 0 1 0 280 628 0 0 0 0 0 331 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X14 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 18 60.84 61.11 3.00 1.00 9.00 TCTTGGTGGAGGTGCCAG POSITION PENALTY, QUALITY: 18000.000000, 18002.835611 RIGHT PRIMER 157 20 60.02 50.00 4.00 2.00 9.00 TGCTAGTGCTGTCAAATGCC POSITION PENALTY, QUALITY: 20000.000000, 20000.018700 SEQUENCE SIZE: 158 INCLUDED REGION SIZE: 158 PRODUCT SIZE: 158, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 0,158 0 TCTTGGTGGAGGTGCCAGCAGCCGAGTCCTTGCAGATGGGATGACTCGTGGCCCAGTTGT ))))))))))))))))))****************************************** 60 GCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGCCTGGCTCGAAACATCTGAAGG ************************************************************ 120 GTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCA ******************(((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 1230 0 0 0 0 0 0 83 898 0 0 0 0 0 249 Right 1230 0 0 0 0 0 0 122 805 0 0 0 0 0 303 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X15 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 23 57.64 39.13 4.00 3.00 11.00 GATTTGCACGTCTACAGAAACTT POSITION PENALTY, QUALITY: 23000.000000, 23005.359396 RIGHT PRIMER 105 21 59.61 38.10 7.00 0.00 9.00 TTTGAAATCATGTTCATCCCC POSITION PENALTY, QUALITY: 21000.000000, 21001.386646 SEQUENCE SIZE: 106 INCLUDED REGION SIZE: 106 PRODUCT SIZE: 106, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 0,106 0 GATTTGCACGTCTACAGAAACTTCATACAAGTATAGCTGGACGCAACCTTTATATCCGTT )))))))))))))))))))))))************************************* 60 TCCAGTCCAGGTCAGGGGATGCCATGGGGATGAACATGATTTCAAA *************************((((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 710 0 0 0 0 0 0 257 250 0 0 0 0 0 203 Right 710 0 0 0 0 0 0 172 343 0 0 0 0 0 195 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X16 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 5 23 57.09 34.78 8.00 3.00 11.00 CAGAGAAAGCACTTTCAAAACTT POSITION PENALTY, QUALITY: 28000.000000, 28005.907183 RIGHT PRIMER 170 20 61.39 55.00 4.00 2.00 9.00 TCTCTGACAACCTTGGCTGG POSITION PENALTY, QUALITY: 20000.000000, 20001.389940 SEQUENCE SIZE: 171 INCLUDED REGION SIZE: 171 PRODUCT SIZE: 166, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 0,171 0 GGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGAAATGCAGATTCTAGC *****)))))))))))))))))))))))******************************** 60 CGTTAGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAGGGAAG ************************************************************ 120 AGGAAAATCTGTTGTTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGA *******************************(((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 1360 0 0 0 0 0 0 684 181 0 8 2 0 0 485 Right 1360 0 0 0 0 0 0 616 248 0 1 0 0 0 495 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X17 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 6 23 57.22 43.48 5.00 3.00 10.00 AAAGACTACCACAGAGGCTATGA POSITION PENALTY, QUALITY: 29000.000000, 29005.780871 RIGHT PRIMER 140 21 59.58 47.62 4.00 2.00 9.00 TGTCCACAGGCAATGTAGATG POSITION PENALTY, QUALITY: 21000.000000, 21001.418918 SEQUENCE SIZE: 141 INCLUDED REGION SIZE: 141 PRODUCT SIZE: 135, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 0,141 0 AGTATTAAAGACTACCACAGAGGCTATGATTGAGGTCAACATTAACAAGAATTTAGTGGG ******)))))))))))))))))))))))******************************* 60 CTCTGCCATGGCTGGGAGCATAGGAGGCTACAACGCCCATGCAGCAAACATTGTCACCGC ************************************************************ 120 CATCTACATTGCCTGTGGACA ((((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 1060 0 0 0 0 1 0 295 528 0 3 0 0 0 233 Right 1060 0 0 0 0 1 0 217 601 0 0 0 0 0 241 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X18 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 20 60.27 50.00 5.00 2.00 9.00 GGATGCAGCACAGAATGTTG POSITION PENALTY, QUALITY: 20000.000000, 20000.272067 RIGHT PRIMER 157 19 62.07 57.89 6.00 1.00 9.00 GCAAACAGGCTTGCTGAGG POSITION PENALTY, QUALITY: 20000.000000, 20003.072251 SEQUENCE SIZE: 159 INCLUDED REGION SIZE: 159 PRODUCT SIZE: 158, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 0,159 0 GGATGCAGCACAGAATGTTGGTAGTTCAAACTGTATTACTTTAATGGAAGCAAGTGGTCC ))))))))))))))))))))**************************************** 60 CACAAATGAAGATTTATATATCAGCTGCACCATGCCATCTATAGAGATAGGAACGGTGGG ************************************************************ 120 TGGTGGGACCAACCTACTACCTCAGCAAGCCTGTTTGCA *******************(((((((((((((((((((* KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 1240 0 0 0 0 18 0 522 395 0 0 2 0 0 303 Right 1240 0 0 0 0 18 0 504 411 0 0 0 0 0 307 Pair Stats: considered 62, high end compl 59, ok 3 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X19 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 21 58.95 52.38 4.00 2.00 9.00 GATGCTAGGTGTTCAAGGAGC POSITION PENALTY, QUALITY: 21000.000000, 21002.049610 RIGHT PRIMER 154 23 58.59 34.78 5.00 0.00 11.00 TGTTGTGAATCATGTGACTTTTG POSITION PENALTY, QUALITY: 23000.000000, 23004.407655 SEQUENCE SIZE: 155 INCLUDED REGION SIZE: 155 PRODUCT SIZE: 155, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 0,155 0 GATGCTAGGTGTTCAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGC )))))))))))))))))))))*************************************** 60 CCGAATTGTGTGTGGGACCGTAATGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGC ************************************************************ 120 AGGACATCTTGTCAAAAGTCACATGATTCACAACA ************((((((((((((((((((((((( KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 1200 0 0 0 0 0 0 119 823 0 0 0 0 0 258 Right 1200 0 0 0 0 0 0 167 784 0 0 0 0 0 249 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 PRIMER PICKING RESULTS FOR HMGCR_X20 Using mispriming library not_for_production.lib Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 0 24 60.24 41.67 4.00 2.00 9.00 GGTCGAAGATCAATTTACAAGACC POSITION PENALTY, QUALITY: 24000.000000, 24004.237346 RIGHT PRIMER 87 19 59.62 52.63 4.00 3.00 8.00 GCCCATGTTCCAGTTCAGA POSITION PENALTY, QUALITY: 260.000000, 261.379041 SEQUENCE SIZE: 164 INCLUDED REGION SIZE: 164 PRODUCT SIZE: 88, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 0,56 0 GGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGAATAG ))))))))))))))))))))))))******************************** 60 CCCGACAGTTCTGAACTGGAACATGGGCATTGGGTTCTAAAGGACTAACATAAAATCTGT <<<<<<<<<<<<<<<<<<< 120 GAATTAAAAAAGCTCAATGCATTGTCTTGTGGAGGATGAATAAA KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high high sid many tar excl ok bad GC too too any 3' lib poly end ered Ns get reg reg GC% clamp low high compl compl sim X stab ok Left 444 0 99 0 0 0 0 91 152 0 0 0 0 0 102 Right 1150 0 0 0 0 42 0 311 512 26 3 0 20 0 236 Pair Stats: considered 1, ok 1 primer3 release 2.0.0 primer3-2.3.6/test/dpal_long_score_output0000664000077300007730000000010112231156245017522 0ustar ioanaioana9.00 1.00 13.00 2.00 13.00 2.00 11.00 11.00 2.00 3.00 3.00 11.00 primer3-2.3.6/test/primer_thal_max_seq_error_output0000664000077300007730000005030412231156245021636 0ustar ioanaioanaP3_COMMENT=Test error in alignment to sequence longer than THAL_MAX_SEQ PRIMER_TASK=pick_detection_primers 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PRIMER_MAX_SELF_END_TH=47.00 PRIMER_INTERNAL_MAX_SELF_END_TH=47.00 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=-1.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_NUM_RETURN=5 PRIMER_PAIR_MAX_COMPL_END_TH=47.00 PRIMER_MAX_SELF_ANY_TH=47.00 PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.00 PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.0 PRIMER_MAX_HAIRPIN_TH=47.00 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_FIRST_BASE_INDEX=1 PRIMER_PRODUCT_SIZE_RANGE=501-600 601-700 401-500 701-850 851-1000 1000-1500 1500-3000 PRIMER_ERROR=Target sequence length > maximum allowed (10000) in thermodynamic alignment (2) = primer3-2.3.6/test/primer_mispriming_th_input0000664000077300007730000000722512231156245020434 0ustar ioanaioanaPRIMER_DNTP_CONC=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 SEQUENCE_ID=exon1 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG SEQUENCE_EXCLUDED_REGION=100,150 PRIMER_PRODUCT_SIZE_RANGE=200-250 PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 PRIMER_PAIR_MAX_DIFF_TM=3 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib = SEQUENCE_ID=exon2 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 = PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_ID=exon2 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 = SEQUENCE_ID=exon1_2 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG SEQUENCE_EXCLUDED_REGION=100,150 PRIMER_PRODUCT_SIZE_RANGE=200-250 PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 PRIMER_PAIR_MAX_DIFF_TM=3 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=16 = SEQUENCE_ID=exon2_2 PRIMER_NUM_RETURN=7 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 = PRIMER_NUM_RETURN=5 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_ID=exon2_3 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=10 = primer3-2.3.6/test/primer_repeat_long_lib0000664000077300007730000046740212231156245017500 0ustar ioanaioana>seq0 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq2 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq3 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq4 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq5 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq6 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq7 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq8 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq9 * 2.0 GGGGGGGGGGGGGGGGGGGYGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq10 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq11 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq12 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq13 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq14 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq15 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq16 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq17 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq18 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq19 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq20 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq21 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq22 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq23 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq24 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq25 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq26 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq27 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq28 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq29 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq30 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq31 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq32 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq33 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq34 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq35 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq36 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq37 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq38 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq39 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq40 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq41 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq42 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq43 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq44 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq45 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq46 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq47 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq48 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq49 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq50 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq51 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq52 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq53 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq54 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq55 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq56 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq57 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq58 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq59 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq60 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq61 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq62 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq63 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq64 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq65 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq66 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq67 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq68 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq69 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq70 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq71 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq72 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq73 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq74 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq75 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq76 * 2.0 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>seq1034 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1035 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1036 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1037 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1038 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1039 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1040 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1041 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1042 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1043 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1044 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1045 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1046 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1047 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1048 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1049 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1050 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1051 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1052 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1053 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1054 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1055 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1056 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1057 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1058 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1059 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1060 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1061 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1062 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1063 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1064 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1065 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1066 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1067 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1068 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1069 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1070 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1071 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1072 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1073 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1074 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1075 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1076 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1077 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1078 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1079 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1080 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1081 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1082 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1083 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1084 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1085 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1086 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1087 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1088 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1089 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1090 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1091 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1092 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1093 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1094 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1095 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1096 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1097 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1098 * 2.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG >seq1099 * 1.0 GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGCATGGCGATCGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG primer3-2.3.6/test/primer_must_use_input0000664000077300007730000001664412231156245017434 0ustar ioanaioanaSEQUENCE_ID=anyway_1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 PRIMER_PICK_ANYWAY=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 = SEQUENCE_ID=high_tm PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = SEQUENCE_ID=high_tm_and_self_any PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = SEQUENCE_ID=high_tm_and_self_any_and_target PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=20,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = SEQUENCE_ID=high_tm_and_self_any_and_excl_region PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 SEQUENCE_EXCLUDED_REGION=20,1 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = SEQUENCE_ID=high_tm_and_self_any_and_incl_region PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 SEQUENCE_INCLUDED_REGION=1,160 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 = SEQUENCE_ID=left_high_tm_and_self_any_and_right PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 = SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc_and_target PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = SEQUENCE_ID=internal_too_short PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccat PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = SEQUENCE_ID=internal_ok PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = SEQUENCE_ID=internal_too_cold PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 = SEQUENCE_ID=internal_too_cold2 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=CCTACAGCTGTGGGAAAATG SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=100 PRIMER_MAX_GC=100 = SEQUENCE_ID=mispriming PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=CCTACAGCTGTGGGAAAATG SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=100 PRIMER_MAX_GC=100 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_MAX_LIBRARY_MISPRIMING=8 = primer3-2.3.6/test/primer_mispriming_th_output0000664000077300007730000011641512231156245020637 0ustar ioanaioanaPRIMER_DNTP_CONC=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 SEQUENCE_ID=exon1 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG SEQUENCE_EXCLUDED_REGION=100,150 PRIMER_PRODUCT_SIZE_RANGE=200-250 PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 PRIMER_PAIR_MAX_DIFF_TM=3 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 877, too many Ns 1, overlap excluded region 91, GC content failed 7, high tm 224, ok 554 PRIMER_RIGHT_EXPLAIN=considered 1012, too many Ns 19, overlap excluded region 58, GC content failed 25, high tm 523, ok 387 PRIMER_PAIR_EXPLAIN=considered 107, unacceptable product size 100, tm diff too large 2, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=7.226308 PRIMER_LEFT_0_PENALTY=2.353081 PRIMER_RIGHT_0_PENALTY=4.873227 PRIMER_LEFT_0_SEQUENCE=TTTCACCCACGAGTGAAG PRIMER_RIGHT_0_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_LEFT_0=41,18 PRIMER_RIGHT_0=279,18 PRIMER_LEFT_0_TM=55.353 PRIMER_RIGHT_0_TM=57.873 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY_TH=16.53 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=30.41 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=3.0200 PRIMER_RIGHT_0_END_STABILITY=4.0200 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=239 PRIMER_PAIR_1_PENALTY=7.556782 PRIMER_LEFT_1_PENALTY=2.683556 PRIMER_RIGHT_1_PENALTY=4.873227 PRIMER_LEFT_1_SEQUENCE=GTTTCACCCACGAGTGAA PRIMER_RIGHT_1_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_LEFT_1=40,18 PRIMER_RIGHT_1=279,18 PRIMER_LEFT_1_TM=55.684 PRIMER_RIGHT_1_TM=57.873 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY_TH=5.03 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=3.67 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=3.1800 PRIMER_RIGHT_1_END_STABILITY=4.0200 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=240 PRIMER_PAIR_2_PENALTY=7.946942 PRIMER_LEFT_2_PENALTY=3.073715 PRIMER_RIGHT_2_PENALTY=4.873227 PRIMER_LEFT_2_SEQUENCE=GTTTCACCCACGAGTGAAG PRIMER_RIGHT_2_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_LEFT_2=40,19 PRIMER_RIGHT_2=279,18 PRIMER_LEFT_2_TM=57.074 PRIMER_RIGHT_2_TM=57.873 PRIMER_LEFT_2_GC_PERCENT=52.632 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY_TH=6.17 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=30.41 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_2_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=3.0200 PRIMER_RIGHT_2_END_STABILITY=4.0200 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=240 PRIMER_PAIR_3_PENALTY=7.999704 PRIMER_LEFT_3_PENALTY=3.126478 PRIMER_RIGHT_3_PENALTY=4.873227 PRIMER_LEFT_3_SEQUENCE=TTTCACCCACGAGTGAAGA PRIMER_RIGHT_3_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_LEFT_3=41,19 PRIMER_RIGHT_3=279,18 PRIMER_LEFT_3_TM=57.126 PRIMER_RIGHT_3_TM=57.873 PRIMER_LEFT_3_GC_PERCENT=47.368 PRIMER_RIGHT_3_GC_PERCENT=61.111 PRIMER_LEFT_3_SELF_ANY_TH=9.06 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=2.41 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=30.41 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_3_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=2.8700 PRIMER_RIGHT_3_END_STABILITY=4.0200 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=239 PRIMER_PAIR_4_PENALTY=8.082008 PRIMER_LEFT_4_PENALTY=3.208781 PRIMER_RIGHT_4_PENALTY=4.873227 PRIMER_LEFT_4_SEQUENCE=GAGTTTCACCCACGAGTG PRIMER_RIGHT_4_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_LEFT_4=38,18 PRIMER_RIGHT_4=279,18 PRIMER_LEFT_4_TM=56.209 PRIMER_RIGHT_4_TM=57.873 PRIMER_LEFT_4_GC_PERCENT=55.556 PRIMER_RIGHT_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY_TH=0.13 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.13 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_4_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=3.5100 PRIMER_RIGHT_4_END_STABILITY=4.0200 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=242 = SEQUENCE_ID=exon2 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 134, high repeat similarity 1, long poly-x seq 23, ok 499 PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 167, high hairpin stability 6, high repeat similarity 4, ok 264 PRIMER_PAIR_EXPLAIN=considered 188, unacceptable product size 179, tm diff too large 2, ok 7 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.475856 PRIMER_LEFT_0_PENALTY=0.096015 PRIMER_RIGHT_0_PENALTY=3.379841 PRIMER_LEFT_0_SEQUENCE=CTTAAGTTTGTAACAGGGCC PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_LEFT_0=21,20 PRIMER_RIGHT_0=267,23 PRIMER_LEFT_0_TM=55.096 PRIMER_RIGHT_0_TM=54.620 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=30.435 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=5.8000 PRIMER_RIGHT_0_END_STABILITY=2.4100 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=247 PRIMER_PAIR_1_PENALTY=3.475856 PRIMER_LEFT_1_PENALTY=0.096015 PRIMER_RIGHT_1_PENALTY=3.379841 PRIMER_LEFT_1_SEQUENCE=CCTTAAGTTTGTAACAGGGC PRIMER_RIGHT_1_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_LEFT_1=20,20 PRIMER_RIGHT_1=267,23 PRIMER_LEFT_1_TM=55.096 PRIMER_RIGHT_1_TM=54.620 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=30.435 PRIMER_LEFT_1_SELF_ANY_TH=1.42 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=5.1900 PRIMER_RIGHT_1_END_STABILITY=2.4100 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=248 PRIMER_PAIR_2_PENALTY=4.300910 PRIMER_LEFT_2_PENALTY=0.096015 PRIMER_RIGHT_2_PENALTY=4.204895 PRIMER_LEFT_2_SEQUENCE=CTTAAGTTTGTAACAGGGCC PRIMER_RIGHT_2_SEQUENCE=ATGATGTCTACTTTCCAAATTACA PRIMER_LEFT_2=21,20 PRIMER_RIGHT_2=268,24 PRIMER_LEFT_2_TM=55.096 PRIMER_RIGHT_2_TM=55.205 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=29.167 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_2_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=2.4100 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=248 PRIMER_PAIR_3_PENALTY=4.300910 PRIMER_LEFT_3_PENALTY=0.096015 PRIMER_RIGHT_3_PENALTY=4.204895 PRIMER_LEFT_3_SEQUENCE=CCTTAAGTTTGTAACAGGGC PRIMER_RIGHT_3_SEQUENCE=ATGATGTCTACTTTCCAAATTACA PRIMER_LEFT_3=20,20 PRIMER_RIGHT_3=268,24 PRIMER_LEFT_3_TM=55.096 PRIMER_RIGHT_3_TM=55.205 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=29.167 PRIMER_LEFT_3_SELF_ANY_TH=1.42 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_3_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=5.1900 PRIMER_RIGHT_3_END_STABILITY=2.4100 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=249 PRIMER_PAIR_4_PENALTY=4.305829 PRIMER_LEFT_4_PENALTY=0.096015 PRIMER_RIGHT_4_PENALTY=4.209814 PRIMER_LEFT_4_SEQUENCE=CTTAAGTTTGTAACAGGGCC PRIMER_RIGHT_4_SEQUENCE=GATGTCTACTTTCCAAATTACA PRIMER_LEFT_4=21,20 PRIMER_RIGHT_4=266,22 PRIMER_LEFT_4_TM=55.096 PRIMER_RIGHT_4_TM=52.790 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=31.818 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_4_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=2.4100 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=246 = PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_ID=exon2 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 134, high repeat similarity 1, long poly-x seq 23, ok 499 PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 167, high hairpin stability 6, high repeat similarity 4, ok 264 PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 1417, long poly-x seq 23, ok 1323 PRIMER_PAIR_EXPLAIN=considered 188, unacceptable product size 179, tm diff too large 2, ok 7 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.475856 PRIMER_LEFT_0_PENALTY=0.096015 PRIMER_RIGHT_0_PENALTY=3.379841 PRIMER_INTERNAL_0_PENALTY=0.542784 PRIMER_LEFT_0_SEQUENCE=CTTAAGTTTGTAACAGGGCC PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_0_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_0=21,20 PRIMER_RIGHT_0=267,23 PRIMER_INTERNAL_0=181,20 PRIMER_LEFT_0_TM=55.096 PRIMER_RIGHT_0_TM=54.620 PRIMER_INTERNAL_0_TM=59.457 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=30.435 PRIMER_INTERNAL_0_GC_PERCENT=65.000 PRIMER_INTERNAL_0_SELF_ANY_TH=1.15 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=27.37 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=5.8000 PRIMER_RIGHT_0_END_STABILITY=2.4100 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=247 PRIMER_PAIR_1_PENALTY=3.475856 PRIMER_LEFT_1_PENALTY=0.096015 PRIMER_RIGHT_1_PENALTY=3.379841 PRIMER_INTERNAL_1_PENALTY=0.542784 PRIMER_LEFT_1_SEQUENCE=CCTTAAGTTTGTAACAGGGC PRIMER_RIGHT_1_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_1_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_1=20,20 PRIMER_RIGHT_1=267,23 PRIMER_INTERNAL_1=181,20 PRIMER_LEFT_1_TM=55.096 PRIMER_RIGHT_1_TM=54.620 PRIMER_INTERNAL_1_TM=59.457 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=30.435 PRIMER_INTERNAL_1_GC_PERCENT=65.000 PRIMER_INTERNAL_1_SELF_ANY_TH=1.15 PRIMER_LEFT_1_SELF_ANY_TH=1.42 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=27.37 PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=5.1900 PRIMER_RIGHT_1_END_STABILITY=2.4100 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=248 PRIMER_PAIR_2_PENALTY=4.300910 PRIMER_LEFT_2_PENALTY=0.096015 PRIMER_RIGHT_2_PENALTY=4.204895 PRIMER_INTERNAL_2_PENALTY=0.542784 PRIMER_LEFT_2_SEQUENCE=CTTAAGTTTGTAACAGGGCC PRIMER_RIGHT_2_SEQUENCE=ATGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_2_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_2=21,20 PRIMER_RIGHT_2=268,24 PRIMER_INTERNAL_2=181,20 PRIMER_LEFT_2_TM=55.096 PRIMER_RIGHT_2_TM=55.205 PRIMER_INTERNAL_2_TM=59.457 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=29.167 PRIMER_INTERNAL_2_GC_PERCENT=65.000 PRIMER_INTERNAL_2_SELF_ANY_TH=1.15 PRIMER_LEFT_2_SELF_ANY_TH=0.00 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=27.37 PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_2_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=2.4100 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=248 PRIMER_PAIR_3_PENALTY=4.300910 PRIMER_LEFT_3_PENALTY=0.096015 PRIMER_RIGHT_3_PENALTY=4.204895 PRIMER_INTERNAL_3_PENALTY=0.542784 PRIMER_LEFT_3_SEQUENCE=CCTTAAGTTTGTAACAGGGC PRIMER_RIGHT_3_SEQUENCE=ATGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_3_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_3=20,20 PRIMER_RIGHT_3=268,24 PRIMER_INTERNAL_3=181,20 PRIMER_LEFT_3_TM=55.096 PRIMER_RIGHT_3_TM=55.205 PRIMER_INTERNAL_3_TM=59.457 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=29.167 PRIMER_INTERNAL_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_SELF_ANY_TH=1.15 PRIMER_LEFT_3_SELF_ANY_TH=1.42 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=27.37 PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_3_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=5.1900 PRIMER_RIGHT_3_END_STABILITY=2.4100 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=249 PRIMER_PAIR_4_PENALTY=4.305829 PRIMER_LEFT_4_PENALTY=0.096015 PRIMER_RIGHT_4_PENALTY=4.209814 PRIMER_INTERNAL_4_PENALTY=0.542784 PRIMER_LEFT_4_SEQUENCE=CTTAAGTTTGTAACAGGGCC PRIMER_RIGHT_4_SEQUENCE=GATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_4_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_4=21,20 PRIMER_RIGHT_4=266,22 PRIMER_INTERNAL_4=181,20 PRIMER_LEFT_4_TM=55.096 PRIMER_RIGHT_4_TM=52.790 PRIMER_INTERNAL_4_TM=59.457 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=31.818 PRIMER_INTERNAL_4_GC_PERCENT=65.000 PRIMER_INTERNAL_4_SELF_ANY_TH=1.15 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=27.37 PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_4_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=2.4100 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=246 = SEQUENCE_ID=exon1_2 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG SEQUENCE_EXCLUDED_REGION=100,150 PRIMER_PRODUCT_SIZE_RANGE=200-250 PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 PRIMER_PAIR_MAX_DIFF_TM=3 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=16 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 877, too many Ns 1, overlap excluded region 91, GC content failed 7, high tm 224, ok 554 PRIMER_RIGHT_EXPLAIN=considered 1012, too many Ns 19, overlap excluded region 58, GC content failed 25, high tm 523, high repeat similarity 1, ok 386 PRIMER_INTERNAL_EXPLAIN=considered 3237, too many Ns 27, GC content failed 32, low tm 155, high tm 94, high hairpin stability 1, ok 2928 PRIMER_PAIR_EXPLAIN=considered 149, unacceptable product size 137, tm diff too large 4, high mispriming library similarity 2, ok 6 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=7.226308 PRIMER_LEFT_0_PENALTY=2.353081 PRIMER_RIGHT_0_PENALTY=4.873227 PRIMER_INTERNAL_0_PENALTY=0.026924 PRIMER_LEFT_0_SEQUENCE=TTTCACCCACGAGTGAAG PRIMER_RIGHT_0_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_INTERNAL_0_SEQUENCE=AGCAGAGGCTGTGCTTGGCG PRIMER_LEFT_0=41,18 PRIMER_RIGHT_0=279,18 PRIMER_INTERNAL_0=142,20 PRIMER_LEFT_0_TM=55.353 PRIMER_RIGHT_0_TM=57.873 PRIMER_INTERNAL_0_TM=59.973 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=65.000 PRIMER_INTERNAL_0_SELF_ANY_TH=26.94 PRIMER_LEFT_0_SELF_ANY_TH=16.53 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=30.41 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=43.71 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=3.0200 PRIMER_RIGHT_0_END_STABILITY=4.0200 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=239 PRIMER_PAIR_1_PENALTY=7.556782 PRIMER_LEFT_1_PENALTY=2.683556 PRIMER_RIGHT_1_PENALTY=4.873227 PRIMER_INTERNAL_1_PENALTY=0.026924 PRIMER_LEFT_1_SEQUENCE=GTTTCACCCACGAGTGAA PRIMER_RIGHT_1_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_INTERNAL_1_SEQUENCE=AGCAGAGGCTGTGCTTGGCG PRIMER_LEFT_1=40,18 PRIMER_RIGHT_1=279,18 PRIMER_INTERNAL_1=142,20 PRIMER_LEFT_1_TM=55.684 PRIMER_RIGHT_1_TM=57.873 PRIMER_INTERNAL_1_TM=59.973 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_INTERNAL_1_GC_PERCENT=65.000 PRIMER_INTERNAL_1_SELF_ANY_TH=26.94 PRIMER_LEFT_1_SELF_ANY_TH=5.03 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=3.67 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=43.71 PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=3.1800 PRIMER_RIGHT_1_END_STABILITY=4.0200 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=240 PRIMER_PAIR_2_PENALTY=7.946942 PRIMER_LEFT_2_PENALTY=3.073715 PRIMER_RIGHT_2_PENALTY=4.873227 PRIMER_INTERNAL_2_PENALTY=0.026924 PRIMER_LEFT_2_SEQUENCE=GTTTCACCCACGAGTGAAG PRIMER_RIGHT_2_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_INTERNAL_2_SEQUENCE=AGCAGAGGCTGTGCTTGGCG PRIMER_LEFT_2=40,19 PRIMER_RIGHT_2=279,18 PRIMER_INTERNAL_2=142,20 PRIMER_LEFT_2_TM=57.074 PRIMER_RIGHT_2_TM=57.873 PRIMER_INTERNAL_2_TM=59.973 PRIMER_LEFT_2_GC_PERCENT=52.632 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_INTERNAL_2_GC_PERCENT=65.000 PRIMER_INTERNAL_2_SELF_ANY_TH=26.94 PRIMER_LEFT_2_SELF_ANY_TH=6.17 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=30.41 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=43.71 PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_2_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=3.0200 PRIMER_RIGHT_2_END_STABILITY=4.0200 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=240 PRIMER_PAIR_3_PENALTY=8.082008 PRIMER_LEFT_3_PENALTY=3.208781 PRIMER_RIGHT_3_PENALTY=4.873227 PRIMER_INTERNAL_3_PENALTY=0.026924 PRIMER_LEFT_3_SEQUENCE=GAGTTTCACCCACGAGTG PRIMER_RIGHT_3_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_INTERNAL_3_SEQUENCE=AGCAGAGGCTGTGCTTGGCG PRIMER_LEFT_3=38,18 PRIMER_RIGHT_3=279,18 PRIMER_INTERNAL_3=142,20 PRIMER_LEFT_3_TM=56.209 PRIMER_RIGHT_3_TM=57.873 PRIMER_INTERNAL_3_TM=59.973 PRIMER_LEFT_3_GC_PERCENT=55.556 PRIMER_RIGHT_3_GC_PERCENT=61.111 PRIMER_INTERNAL_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_SELF_ANY_TH=26.94 PRIMER_LEFT_3_SELF_ANY_TH=0.13 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.13 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=43.71 PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_3_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=3.5100 PRIMER_RIGHT_3_END_STABILITY=4.0200 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=242 PRIMER_PAIR_4_PENALTY=8.285030 PRIMER_LEFT_4_PENALTY=2.683556 PRIMER_RIGHT_4_PENALTY=5.601474 PRIMER_INTERNAL_4_PENALTY=0.026924 PRIMER_LEFT_4_SEQUENCE=GTTTCACCCACGAGTGAA PRIMER_RIGHT_4_SEQUENCE=GCTCCTCAGCCTTGTCAC PRIMER_INTERNAL_4_SEQUENCE=AGCAGAGGCTGTGCTTGGCG PRIMER_LEFT_4=40,18 PRIMER_RIGHT_4=280,18 PRIMER_INTERNAL_4=142,20 PRIMER_LEFT_4_TM=55.684 PRIMER_RIGHT_4_TM=58.601 PRIMER_INTERNAL_4_TM=59.973 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=61.111 PRIMER_INTERNAL_4_GC_PERCENT=65.000 PRIMER_INTERNAL_4_SELF_ANY_TH=26.94 PRIMER_LEFT_4_SELF_ANY_TH=5.03 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=3.67 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=40.78 PRIMER_INTERNAL_4_HAIRPIN_TH=43.71 PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_4_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=3.1800 PRIMER_RIGHT_4_END_STABILITY=3.6700 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=241 = SEQUENCE_ID=exon2_2 PRIMER_NUM_RETURN=7 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 134, high repeat similarity 2, long poly-x seq 23, ok 498 PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 167, high hairpin stability 6, high repeat similarity 8, ok 260 PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 1417, long poly-x seq 23, ok 1323 PRIMER_PAIR_EXPLAIN=considered 495, unacceptable product size 446, tm diff too large 24, high mispriming library similarity 17, ok 8 PRIMER_LEFT_NUM_RETURNED=7 PRIMER_RIGHT_NUM_RETURNED=7 PRIMER_INTERNAL_NUM_RETURNED=7 PRIMER_PAIR_NUM_RETURNED=7 PRIMER_PAIR_0_PENALTY=4.767068 PRIMER_LEFT_0_PENALTY=1.387227 PRIMER_RIGHT_0_PENALTY=3.379841 PRIMER_INTERNAL_0_PENALTY=0.542784 PRIMER_LEFT_0_SEQUENCE=ACCTTAAGTTTGTAACAGGG PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_0_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_0=19,20 PRIMER_RIGHT_0=267,23 PRIMER_INTERNAL_0=181,20 PRIMER_LEFT_0_TM=53.613 PRIMER_RIGHT_0_TM=54.620 PRIMER_INTERNAL_0_TM=59.457 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=30.435 PRIMER_INTERNAL_0_GC_PERCENT=65.000 PRIMER_INTERNAL_0_SELF_ANY_TH=1.15 PRIMER_LEFT_0_SELF_ANY_TH=1.83 PRIMER_RIGHT_0_SELF_ANY_TH=0.00 PRIMER_INTERNAL_0_SELF_END_TH=0.00 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_INTERNAL_0_HAIRPIN_TH=27.37 PRIMER_LEFT_0_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=4.4500 PRIMER_RIGHT_0_END_STABILITY=2.4100 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=249 PRIMER_PAIR_1_PENALTY=4.829150 PRIMER_LEFT_1_PENALTY=1.449308 PRIMER_RIGHT_1_PENALTY=3.379841 PRIMER_INTERNAL_1_PENALTY=0.542784 PRIMER_LEFT_1_SEQUENCE=AACCTTAAGTTTGTAACAGGG PRIMER_RIGHT_1_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_1_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_1=18,21 PRIMER_RIGHT_1=267,23 PRIMER_INTERNAL_1=181,20 PRIMER_LEFT_1_TM=54.551 PRIMER_RIGHT_1_TM=54.620 PRIMER_INTERNAL_1_TM=59.457 PRIMER_LEFT_1_GC_PERCENT=38.095 PRIMER_RIGHT_1_GC_PERCENT=30.435 PRIMER_INTERNAL_1_GC_PERCENT=65.000 PRIMER_INTERNAL_1_SELF_ANY_TH=1.15 PRIMER_LEFT_1_SELF_ANY_TH=1.83 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_INTERNAL_1_SELF_END_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=34.81 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_INTERNAL_1_HAIRPIN_TH=27.37 PRIMER_LEFT_1_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=4.4500 PRIMER_RIGHT_1_END_STABILITY=2.4100 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=250 PRIMER_PAIR_2_PENALTY=4.841351 PRIMER_LEFT_2_PENALTY=1.461510 PRIMER_RIGHT_2_PENALTY=3.379841 PRIMER_INTERNAL_2_PENALTY=0.542784 PRIMER_LEFT_2_SEQUENCE=TTAAGTTTGTAACAGGGCCC PRIMER_RIGHT_2_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_2_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_2=22,20 PRIMER_RIGHT_2=267,23 PRIMER_INTERNAL_2=181,20 PRIMER_LEFT_2_TM=56.462 PRIMER_RIGHT_2_TM=54.620 PRIMER_INTERNAL_2_TM=59.457 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=30.435 PRIMER_INTERNAL_2_GC_PERCENT=65.000 PRIMER_INTERNAL_2_SELF_ANY_TH=1.15 PRIMER_LEFT_2_SELF_ANY_TH=17.05 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_INTERNAL_2_SELF_END_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=17.05 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_INTERNAL_2_HAIRPIN_TH=27.37 PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_2_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=2.4100 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=246 PRIMER_PAIR_3_PENALTY=4.956287 PRIMER_LEFT_3_PENALTY=1.576445 PRIMER_RIGHT_3_PENALTY=3.379841 PRIMER_INTERNAL_3_PENALTY=0.542784 PRIMER_LEFT_3_SEQUENCE=TAAGTTTGTAACAGGGCCC PRIMER_RIGHT_3_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_3_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_3=23,19 PRIMER_RIGHT_3=267,23 PRIMER_INTERNAL_3=181,20 PRIMER_LEFT_3_TM=55.576 PRIMER_RIGHT_3_TM=54.620 PRIMER_INTERNAL_3_TM=59.457 PRIMER_LEFT_3_GC_PERCENT=47.368 PRIMER_RIGHT_3_GC_PERCENT=30.435 PRIMER_INTERNAL_3_GC_PERCENT=65.000 PRIMER_INTERNAL_3_SELF_ANY_TH=1.15 PRIMER_LEFT_3_SELF_ANY_TH=17.05 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_INTERNAL_3_SELF_END_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=17.05 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_INTERNAL_3_HAIRPIN_TH=27.37 PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_3_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=5.8000 PRIMER_RIGHT_3_END_STABILITY=2.4100 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=245 PRIMER_PAIR_4_PENALTY=5.121610 PRIMER_LEFT_4_PENALTY=1.741769 PRIMER_RIGHT_4_PENALTY=3.379841 PRIMER_INTERNAL_4_PENALTY=0.542784 PRIMER_LEFT_4_SEQUENCE=TTTGTAACAGGGCCCAAAT PRIMER_RIGHT_4_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_4_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_4=27,19 PRIMER_RIGHT_4=267,23 PRIMER_INTERNAL_4=181,20 PRIMER_LEFT_4_TM=55.742 PRIMER_RIGHT_4_TM=54.620 PRIMER_INTERNAL_4_TM=59.457 PRIMER_LEFT_4_GC_PERCENT=42.105 PRIMER_RIGHT_4_GC_PERCENT=30.435 PRIMER_INTERNAL_4_GC_PERCENT=65.000 PRIMER_INTERNAL_4_SELF_ANY_TH=1.15 PRIMER_LEFT_4_SELF_ANY_TH=13.19 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_INTERNAL_4_SELF_END_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.97 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_INTERNAL_4_HAIRPIN_TH=27.37 PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_4_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=2.3200 PRIMER_RIGHT_4_END_STABILITY=2.4100 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=241 PRIMER_PAIR_5_PENALTY=5.592121 PRIMER_LEFT_5_PENALTY=1.387227 PRIMER_RIGHT_5_PENALTY=4.204895 PRIMER_INTERNAL_5_PENALTY=0.542784 PRIMER_LEFT_5_SEQUENCE=ACCTTAAGTTTGTAACAGGG PRIMER_RIGHT_5_SEQUENCE=ATGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_5_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_5=19,20 PRIMER_RIGHT_5=268,24 PRIMER_INTERNAL_5=181,20 PRIMER_LEFT_5_TM=53.613 PRIMER_RIGHT_5_TM=55.205 PRIMER_INTERNAL_5_TM=59.457 PRIMER_LEFT_5_GC_PERCENT=40.000 PRIMER_RIGHT_5_GC_PERCENT=29.167 PRIMER_INTERNAL_5_GC_PERCENT=65.000 PRIMER_INTERNAL_5_SELF_ANY_TH=1.15 PRIMER_LEFT_5_SELF_ANY_TH=1.83 PRIMER_RIGHT_5_SELF_ANY_TH=0.00 PRIMER_INTERNAL_5_SELF_END_TH=0.00 PRIMER_LEFT_5_SELF_END_TH=0.00 PRIMER_RIGHT_5_SELF_END_TH=0.00 PRIMER_LEFT_5_HAIRPIN_TH=0.00 PRIMER_RIGHT_5_HAIRPIN_TH=0.00 PRIMER_INTERNAL_5_HAIRPIN_TH=27.37 PRIMER_LEFT_5_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_5_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_5_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_5_END_STABILITY=4.4500 PRIMER_RIGHT_5_END_STABILITY=2.4100 PRIMER_PAIR_5_COMPL_ANY_TH=0.00 PRIMER_PAIR_5_COMPL_END_TH=0.00 PRIMER_PAIR_5_PRODUCT_SIZE=250 PRIMER_PAIR_6_PENALTY=5.597041 PRIMER_LEFT_6_PENALTY=1.387227 PRIMER_RIGHT_6_PENALTY=4.209814 PRIMER_INTERNAL_6_PENALTY=0.542784 PRIMER_LEFT_6_SEQUENCE=ACCTTAAGTTTGTAACAGGG PRIMER_RIGHT_6_SEQUENCE=GATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_6_SEQUENCE=TGAGGACTGGGTGCTGGCCT PRIMER_LEFT_6=19,20 PRIMER_RIGHT_6=266,22 PRIMER_INTERNAL_6=181,20 PRIMER_LEFT_6_TM=53.613 PRIMER_RIGHT_6_TM=52.790 PRIMER_INTERNAL_6_TM=59.457 PRIMER_LEFT_6_GC_PERCENT=40.000 PRIMER_RIGHT_6_GC_PERCENT=31.818 PRIMER_INTERNAL_6_GC_PERCENT=65.000 PRIMER_INTERNAL_6_SELF_ANY_TH=1.15 PRIMER_LEFT_6_SELF_ANY_TH=1.83 PRIMER_RIGHT_6_SELF_ANY_TH=0.00 PRIMER_INTERNAL_6_SELF_END_TH=0.00 PRIMER_LEFT_6_SELF_END_TH=0.00 PRIMER_RIGHT_6_SELF_END_TH=0.00 PRIMER_LEFT_6_HAIRPIN_TH=0.00 PRIMER_RIGHT_6_HAIRPIN_TH=0.00 PRIMER_INTERNAL_6_HAIRPIN_TH=27.37 PRIMER_LEFT_6_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_6_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_6_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_6_END_STABILITY=4.4500 PRIMER_RIGHT_6_END_STABILITY=2.4100 PRIMER_PAIR_6_COMPL_ANY_TH=0.00 PRIMER_PAIR_6_COMPL_END_TH=0.00 PRIMER_PAIR_6_PRODUCT_SIZE=248 = PRIMER_NUM_RETURN=5 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_ID=exon2_3 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=10 PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 134, high hairpin stability 6, high repeat similarity 153, long poly-x seq 23, ok 341 PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 167, high hairpin stability 97, high repeat similarity 126, ok 51 PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 1417, long poly-x seq 23, ok 1323 PRIMER_PAIR_EXPLAIN=considered 20759, unacceptable product size 11888, tm diff too large 5556, high mispriming library similarity 3315, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = primer3-2.3.6/test/primer_must_overlap_point_input0000664000077300007730000004064212231156245021514 0ustar ioanaioanaSEQUENCE_ID=Medicago Lyk3 mRNA regular pick SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 = SEQUENCE_ID=Medicago Lyk3 mRNA creates out of sequence error big SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=646 680 838 1041 1133 1228 1382 1469 1608 1706 2091 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 = SEQUENCE_ID=Medicago Lyk3 mRNA creates out of sequence error small SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=0 646 680 838 1041 1133 1228 1382 1469 1608 1706 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 = SEQUENCE_ID=Medicago Lyk3 mRNA picks primers overlapping one intron SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=646 680 838 1041 1133 1228 1382 1469 1608 1706 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 = SEQUENCE_ID=Medicago Lyk3 mRNA picks primers overlapping one intron with included region SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=646 680 838 1041 1133 1228 1382 1469 1608 1706 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 SEQUENCE_INCLUDED_REGION=600,1400 = SEQUENCE_ID=Medicago Lyk3 mRNA picks primers overlapping one intron one pair SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=646 680 838 1041 1133 1228 1382 1469 1608 1706 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_THREE_PRIME_DISTANCE=10 = SEQUENCE_ID=Medicago Lyk3 mRNA picks primers overlapping one intron one pair SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaagattaatgtcactgtaatttgttcatgtgggaatagccagatttcaaaagattatggcttgtttgttacctatccactcaggtctgatgatactcttgcgaaaattgcgaccaaagctggtcttgatgaagggttgatacaaaatttcaatcaagatgccaatttcagcataggaagtgggatagtgttcattccaggaagagatcaaaatggacatttttttcctttgtattctagaacaggtattgctaagggttcagctgttggtatagctatggcaggaatatttggacttctattatttgttatctatatatatgccaaatacttccaaaagaaggaagaagagaaaactaaacttccacaaacttctagggcattttcaactcaagatgcctcaggtagtgcagaatatgaaacttcaggatccagtgggcatgctactggtagtgctgccggccttacaggcattatggtggcaaagtcgacagagtttacgtatcaagaattagccaaggcgacaaataatttcagcttggataataaaattggtcaaggtggatttggagctgtctattatgcagaacttagaggcgagaaaacagcaattaagaagatggatgtacaagcatcgtccgaatttctctgtgagttgaaggtcttaacacatgttcatcacttgaatctggtgcggttgattggatattgcgttgaagggtcacttttcctcgtatatgaacatattgacaatggaaacttgggtcaatatttacatggtataggtacagaaccattaccatggtctagtagagtgcagattgctctagattcagccagaggcctagaatacattcatgaacacactgtgcctgtttatatccatcgcgacgtaaaatcagcaaatatattgatagacaaaaatttgcgtggaaaggttgctgattttggcttgaccaaacttattgaagttggaaactcgacacttcacactcgtcttgtgggaacatttggatacatgccaccagaatatgctcaatatggcgatgtttctccaaaaatagatgtatatgcttttggcgttgttctttatgaacttattactgcaaagaatgctgtcctgaagacaggtgaatctgttgcagaatcaaagggtcttgtacaattgtttgaagaagcacttcatcgaatggatcctttagaaggtcttcgaaaattggtggatcctaggcttaaagaaaactatcccattgattctgttctcaagatggctcaacttgggagagcatgtacgagagacaatccgctactacgcccaagcatgagatctatagttgttgctcttatgacactttcatcaccaactgaagattgtgatgatgactcttcatatgaaaatcaatctctcataaatctgttgtcaactagatgaagattttgtgtgacaaattgaattgtgtttgttaaaacatgtagaaagcatacaacaaatggtttgtactttacttgtatatgaaatattgcagttggagagtttttacttttcttacctcaattatccatcttgaacattgttttgtatgtggcaagagttcaaacactggtgtactcattgaaaagttatggtgagaaaatcactgatcagatgat SEQUENCE_OVERLAP_JUNCTION_LIST=1133 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_THREE_PRIME_DISTANCE=10 = primer3-2.3.6/test/thal_long_input0000775000077300007730000004437712231156245016170 0ustar ioanaioana-maxloop 1 -s1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTC -s2 ACGTACGTAGGGCTAGCTAG -a ANY -maxlopp 1 -s1 GGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTTCGTGCGTGCCAGTATTACACCTATAATCACAAGACCCCTTCAATACGAGTCCTGTGGCGTAGTGTTCCATCAAAACAATCAAGAACAGATTTCCGGTCCCCGTTGTGTTGGGATCTAGCGGACGTTGTCGGTAGATCAATAACGTAAATGCGAATCGAAGTTCTCTGGCCTAAAACAACTGCGCGCAGGGCCTCCGGTCATTGCATCTTTCTTGTCTCTCGTGAGGGCGTGATTCGTTTACCTGGAGCGAGCCGGGCACAAGAGCTATGGATTATTGGCTGGTGCAAAAACCATTCTAGCTACAATTATACTCGCGTGTCGACGATAAGAGTGAAATCACTGCGTAGG -s2 ACGTACGTAGGGCTAGCTAG-a ANY -maxloop 1 -s1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATG -s2 ACGTGTTCGTCGTAAATAACATGCTATAT -a ANY -maxloop 1 -s1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAAT -s2 ACGTGTTCGTCGTAAATAACATGCTATATTA -s1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTT -s2 ACGTGTTCGTCGTAAATAACATGCTATATT -a END1 -s1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAACCTGGTGACTCCCTTAGCCAATTGTGTAACTT -s2 ACGTGTTCGTCGTAAATAACATGCTATATT -a END2 -s1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACC -s2 ACGTGTTCGTCGTAAATAACATGCTATATT -s1 ACGTGTTCGTCGTAAATAACATGCTATATT -s2 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGAGAGGCTGATACATGCCCTAGATGGACGGGTCCAGTCTACGACATATTTCCCTCGACTGCGCGGTTCACCC -a END2 -maxloop 5 -maxloop 1 -s1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCATAGCCAAGGGACCCAGCTGTTCCTTAGAAAC -s2 ACGTACGTAGGGCTAGCTAG -a ANY -maxloop 1 -s1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATT -maxloop 0 -s1 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGT ACGTGTTCGTCGTAAATAACATGCTATATT -a ANY -s1 ACGTGTTCGTCGTAAATAACATGCTATATT -s2 GACGTAGACAACCCTGTGTTTAGCCTGCGTTTTGTGCCATCCTAATGCTTTACTAGATCACTGAGCCACCTCCCAAGGACTACACCTAGCGGTATTTCGTACATTAACTAGGATCCTTTTCCACATGGACTACAATGTCTGCCGAGCATGCGATGGGGTACCGCGCCCGCGCACATACGCGCGCAGAGCTTTTGGAGGCATACCTACACCGGCGAGGGGCTGCGGTTTATTGACACTGAAACGGGATAACGAGTCGCTGAATTGAGCCAAAAATATGCAAGCGTCACAAATTGTGACAAAAATTTTAAAGGAAAAATTAGACCATTGATTCTGAAGTGGTGCGTATAGGACCAGTCGTGGCAATGAGACCGATTTGAGTAGCACTAGCTCAAACACTGTCTGGGTCGCCATCAAGGCCACAAGAACTTAAGCAGCCGTCACCCTATAGAAGGTTAAGCGACGGTTAGGGCTTCTGGCAACGAAAGTTGTCGGTTCGTCCTGTGCCAACGTGTGGCAAAGTCTACTATGATTCGATTGTTGACGTGTCGACAGGCTGTTTCGCTGGATACCCCACCTTGATAATTTTTCTCGTCGAACGCTAGCAGTTTTTTTTTCAACGGCCCGGAATCTGTAAGAGGCCGTTGCAGGAACGCGTGTGTATGTAAATGCCCACTACTTCTGTTATGTACCCAAATGGCGTGCGGCGTGGATGTATAGTGTCGACCCTCCATAATCGGGCGGACGGTCGTGGGGTATGTATGATCTTCGGCACTGATTCGCCTCGAGTCTATATGTTCTTAATCCAGACCTTCGGGGAAAGCCTACTTTCCATCCGTTGTCTAGCGTCATGCCAGTGACTACTGTTGTATTGTCTGGTTCCTAAGATAGCCATGGATTCCGGACATCGACGATGCACAAGAGCGTTAGCGCTGGTGTGCAACGCAACGTCGCGAAGGCTGGGTTACAGCGTGATCTCCTGGCTGCACCCAGATGCAGAGGGACATACCTACGATGAATAGGTGCGTCTGTTTATAAACGCCCAATCCTAGCAAAAATCACAACTAAGACAGTGTATGGAAGACCCACCAGTTGTGGGCGAATGGTCAGGTATACAAGATCGTGTCAAGACGGAACTTAAGCTTCTGTGCGCTCTCCATGCGAGCTGGTACGTCTGGACGGCGAGGTATGAGTGAATGACCATCCATGGCAACTTTCGTGTTCTACGACAGATACGAGCTCGACGGACGACCTGGTGACCAGTAGTATATGCGCGTCCGTCGGCCAGACTTTCCAAACGCCCTTTCAACGAGATACATGCGAACACGCTACAATTTCTCGTTCCGTCTAAAGTCGATACTCGCAAGCCCAGGCCCGTTACTACAACGCTGTTAATAGGATCAGAAGGGCCATAAGACTTTGGCAGCGGTAGCTAGGAAAGTGATGGTTGTGATGGCCCTAGTAAGGAGTCAGCCATCTACCCAACTATTTGAATGGGACCA -a END2 primer3-2.3.6/test/primer_boundary1_formatted_output0000664000077300007730000033536612231156245021747 0ustar ioanaioanaPRIMER PICKING RESULTS FOR boundary_test_00 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_01 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_02 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_03 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, unacceptable product size 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_04 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, unacceptable product size 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_05 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 24 60.24 33.33 12.00 10.00 CACGTCATCTCATCAAATTTGATT RIGHT PRIMER 57 24 59.91 37.50 4.00 1.00 GAATTGTTTGGAAATGACAGTGAG SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Right 5 3 0 0 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 4, unacceptable product size 3, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_06 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 5 0 0 0 0 0 0 0 0 0 0 0 0 Right 5 5 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_07 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_08 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ... . KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 6 6 0 0 0 0 0 0 0 0 0 0 0 0 Right 6 5 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_09 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 54 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. ... KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 5 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_10 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 1 NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 5 0 0 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_11 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 61.18 33.33 12.00 9.50 CACGTCATCTNATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.75 1 NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 2 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 3, unacceptable product size 2, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_11 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 1 NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 5 0 0 0 0 0 0 0 0 0 0 0 0 Right 5 2 0 0 0 0 0 0 0 0 0 0 0 3 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_12 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 63.22 34.78 12.00 11.00 CACGTCATCNNATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 0 0 0 0 0 0 0 0 0 0 0 0 5 Pair Stats: considered 5, unacceptable product size 4, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_13 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 55.84 0.00 0.00 0.00 AAAAAAAAAAAAAAAAAAAAAAAAA RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 1 NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_14 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 1 NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 1 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_15 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 90.45 100.00 0.00 0.00 GGGGGGGGGGGGGGGGGGGGGGGGG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 1.00, PAIR 3' COMPL: 0.00 1 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_16 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 1 NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 1 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 1 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_17 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_18 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ... . KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 1 0 0 0 0 0 0 0 0 0 0 0 0 Right 1 1 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_19 INPUT PROBLEM: SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE PRIMER PICKING RESULTS FOR boundary_test_20 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 54 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. ... KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 1 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_21 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 28,5 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_22 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 27,6 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ****** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 2 2 0 0 0 0 0 0 0 0 0 0 0 0 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_23 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 28,6 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ****** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 2 2 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_24 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 29,5 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ***** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 2 2 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_25 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 28,1 29,4 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_26 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 27,1 28,5 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ****** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 1 0 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_27 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 28,1 29,5 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ****** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 1 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_28 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 29,1 30,4 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ***** KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 1 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_29 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> * <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_30 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 29,0 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 4 3 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_31 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 1,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN * KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, no target 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_32 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 28,5 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>>XXXXX<<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_33 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 27,6 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_34 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 28,6 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_35 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 29,5 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_36 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 28,1 29,4 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>>XXXXX<<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_37 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 27,1 28,5 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_37 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 28,1 29,5 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_38 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 29,1 30,4 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXX KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_39 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_40 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 29,0 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_41 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 11,0 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_42 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_43 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN X KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 1 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_44 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_45 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_46 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN X KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 1 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_47 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_48 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN X KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 1 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_49 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_50 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN X KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 1 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_51 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_52 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_53 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN X KEYS (in order of precedence): XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, tm diff too large 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_54 No mispriming library specified Using 1-based sequence positions WARNING: Unrecognized base in input sequence OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 63.02 40.00 4.00 0.00 GAATTGTTTGGAAATGACAGTGAGG SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00 EXCLUDED REGIONS (start, len)*: 29,1 1 NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> X <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_55 INPUT PROBLEM: Unrecognized base in input sequence PRIMER PICKING RESULTS FOR poly_x_1 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 187 INCLUDED REGION SIZE: 187 1 AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGG 61 TTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTT 121 GGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTT 181 TCCCCCC Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 138 0 0 0 0 0 0 0 0 0 0 138 0 0 Right 138 0 0 0 0 0 0 0 0 0 0 138 0 0 Intl 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR poly_x_2 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 30 25 67.77 40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC RIGHT PRIMER 131 25 67.77 40.00 12.00 3.00 GAAAAATTTTTCCCCCAAAAAGGGG INTERNAL OLIGO 88 19 60.50 47.37 5.00 3.00 CCCTTTTTGGGGGAAAAAC SEQUENCE SIZE: 180 INCLUDED REGION SIZE: 180 PRODUCT SIZE: 102, PAIR ANY COMPL: 22.00, PAIR 3' COMPL: 14.00 1 AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGG >>>>>>>>>>>>>>>>>>>>>>>>> 61 AAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG ^^^^^^^^^^^^^^^^^^^<<<<<<<<<<<<<< 121 AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG <<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ^^^^^^ internal oligo ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 30 25 67.77 40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC RIGHT PRIMER 132 25 67.77 40.00 14.00 1.00 GGAAAAATTTTTCCCCCAAAAAGGG INTERNAL OLIGO 89 19 60.50 47.37 5.00 1.00 CCTTTTTGGGGGAAAAACC PRODUCT SIZE: 103, PAIR ANY COMPL: 23.00, PAIR 3' COMPL: 19.00 2 LEFT PRIMER 30 25 67.77 40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC RIGHT PRIMER 133 25 67.77 40.00 16.00 1.00 GGGAAAAATTTTTCCCCCAAAAAGG INTERNAL OLIGO 90 19 60.50 47.37 5.00 1.00 CTTTTTGGGGGAAAAACCC PRODUCT SIZE: 104, PAIR ANY COMPL: 24.00, PAIR 3' COMPL: 22.00 3 LEFT PRIMER 30 25 67.77 40.00 18.00 18.00 CTTTTTGGGGGAAAAATTTTTCCCC RIGHT PRIMER 134 25 67.77 40.00 18.00 3.00 GGGGAAAAATTTTTCCCCCAAAAAG INTERNAL OLIGO 90 19 60.50 47.37 5.00 1.00 CTTTTTGGGGGAAAAACCC PRODUCT SIZE: 105, PAIR ANY COMPL: 25.00, PAIR 3' COMPL: 25.00 4 LEFT PRIMER 29 25 67.77 40.00 16.00 16.00 CCTTTTTGGGGGAAAAATTTTTCCC RIGHT PRIMER 131 25 67.77 40.00 12.00 3.00 GAAAAATTTTTCCCCCAAAAAGGGG INTERNAL OLIGO 88 19 60.50 47.37 5.00 3.00 CCCTTTTTGGGGGAAAAAC PRODUCT SIZE: 103, PAIR ANY COMPL: 23.00, PAIR 3' COMPL: 19.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 131 0 0 0 0 0 0 0 0 0 0 0 0 131 Right 131 0 0 0 0 0 0 0 0 0 0 0 0 131 Intl 1585 0 0 0 0 0 0 0 0 0 0 0 0 1585 Pair Stats: considered 64, unacceptable product size 48, ok 16 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR poly_x_3 No mispriming library specified No internal oligo mishyb library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 204 INCLUDED REGION SIZE: 204 1 AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCC 61 TTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGG 121 GGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCC 181 CCCTTTTTTTGGGGGGGGGGGGGG Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 155 0 0 0 0 0 0 0 0 0 0 57 0 98 Right 155 0 0 0 0 0 0 0 0 0 0 72 0 83 Intl 187 0 0 0 0 0 0 0 0 0 0 187 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_59 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 28,0 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_60 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 EXCLUDED REGIONS (start, len)*: 27,0 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 1 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_61 INPUT PROBLEM: Negative EXCLUDED_REGION length PRIMER PICKING RESULTS FOR boundary_test_62 No mispriming library specified Using 1-based sequence positions WARNING: EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 2,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_63 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 EXCLUDED REGIONS (start, len)*: 3,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..X .. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 1 0 0 0 0 0 0 0 0 0 0 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_64 INPUT PROBLEM: EXCLUDED_REGION beyond end of sequence PRIMER PICKING RESULTS FOR boundary_test_65 No mispriming library specified Using 1-based sequence positions WARNING: EXCLUDED_REGION outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 EXCLUDED REGIONS (start, len)*: 59,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_66 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 EXCLUDED REGIONS (start, len)*: 57,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. X.. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_67 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND WARNING: EXCLUDED_REGION outside of INCLUDED_REGION SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 EXCLUDED REGIONS (start, len)*: 57,2 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. X.. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 6 5 0 0 0 0 0 0 0 0 0 0 0 1 Right 6 5 0 1 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_68 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 28,0 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_69 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 27,0 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 2 2 0 0 0 0 0 0 0 0 0 0 0 0 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_70 INPUT PROBLEM: Negative TARGET length PRIMER PICKING RESULTS FOR boundary_test_71 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND WARNING: TARGET outside of INCLUDED_REGION SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 TARGETS (start, len)*: 2,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. .. KEYS (in order of precedence): ...... vector sequence ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, no target 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_72 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 TARGETS (start, len)*: 3,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..* .. KEYS (in order of precedence): ...... vector sequence ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 1 0 0 0 0 0 0 0 0 0 0 0 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_73 INPUT PROBLEM: TARGET beyond end of sequence PRIMER PICKING RESULTS FOR boundary_test_74 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND WARNING: TARGET outside of INCLUDED_REGION SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 TARGETS (start, len)*: 59,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. .. KEYS (in order of precedence): ...... vector sequence ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, no target 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_75 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 TARGETS (start, len)*: 57,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. *.. KEYS (in order of precedence): ...... vector sequence ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_76 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND WARNING: TARGET outside of INCLUDED_REGION SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 TARGETS (start, len)*: 57,2 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. *.. KEYS (in order of precedence): ...... vector sequence ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 6 5 0 0 0 0 0 0 0 0 0 0 0 1 Right 6 5 1 0 0 0 0 0 0 0 0 0 0 0 Pair Stats: considered 0, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_77 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 28,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_78 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 28,5 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>>*****<<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_79 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 28,0 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 6 5 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_80 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 32,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> *<<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_81 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 33,0 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 6 5 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_82 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 33,0 1,1 59,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN * >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< * KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_83 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 TARGETS (start, len)*: 1,1 59,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN * * KEYS (in order of precedence): ****** target Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, no target 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_84 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 1,1 33,0 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN * >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 8 7 0 0 0 0 0 0 0 0 0 0 0 1 Right 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_85 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 59 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 33,0 59,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN >>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< * KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 10 9 0 0 0 0 0 0 0 0 0 0 0 1 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_86 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 35 62.84 25.71 12.00 10.00 CACGTCATCTATATATATATCATCAAATTTGATTG RIGHT PRIMER 77 35 61.36 25.71 10.00 8.00 GAATTGTTTGGAAATGACAGTATATATATATGAGT SEQUENCE SIZE: 79 INCLUDED REGION SIZE: 79 PRODUCT SIZE: 75, PAIR ANY COMPL: 13.00, PAIR 3' COMPL: 8.00 1 NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTG >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<< 61 TCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<< KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Right 5 4 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_71 No mispriming library specified Using 1-based sequence positions WARNING: TARGET outside of INCLUDED_REGION OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 2,1 28,1 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_72 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 56 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< . KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 3, unacceptable product size 2, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_73 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 56 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN .. . KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 2, unacceptable product size 2, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_74 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 56 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< . KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Right 3 2 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_75 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 2 1 0 0 0 0 0 0 0 0 0 0 0 1 Right 2 1 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 2, unacceptable product size 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_76 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 56 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< . KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 2 1 0 0 0 0 0 0 0 0 0 0 0 1 Right 2 1 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 2, unacceptable product size 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_77 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 86 INCLUDED REGION SIZE: 82 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< 61 CTCACTGTCATTTCCAAACAATTCNN .. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 84 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 28 27 0 0 0 0 0 0 0 0 0 0 0 1 Right 28 26 0 0 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 4, unacceptable product size 2, ok 2 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_78 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 84 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 86 INCLUDED REGION SIZE: 82 PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA ..>>>>>>>>>>>>>>>>>>>>>>>>> < 61 CTCACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 28 27 0 0 0 0 0 0 0 0 0 0 0 1 Right 28 26 0 0 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 4, unacceptable product size 2, ok 2 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_79 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 86 INCLUDED REGION SIZE: 82 PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<< 61 CTCACTGTCATTTCCAAACAATTCNN .. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 84 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 28 27 0 0 0 0 0 0 0 0 0 0 0 1 Right 28 26 0 0 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 3, unacceptable product size 1, ok 2 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_80 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 84 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT SEQUENCE SIZE: 86 INCLUDED REGION SIZE: 82 PRODUCT SIZE: 82, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 1 NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNA ..>>>>>>>>>>>>>>>>>>>>>>>>> < 61 CTCACTGTCATTTCCAAACAATTCNN <<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' seq 1 LEFT PRIMER 3 25 62.73 36.00 12.00 10.00 CACGTCATCTCATCAAATTTGATTG RIGHT PRIMER 57 25 60.65 36.00 4.00 3.00 GAATTGTTTGGAAATGACAGTGAGT PRODUCT SIZE: 55, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 28 27 0 0 0 0 0 0 0 0 0 0 0 1 Right 28 26 0 0 0 0 0 0 0 0 0 0 0 2 Pair Stats: considered 3, unacceptable product size 1, ok 2 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_81 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG RIGHT PRIMER 57 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_82 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN .. .. KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, high any compl 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_83 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG RIGHT PRIMER 57 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_84 No mispriming library specified Using 1-based sequence positions NO PRIMERS FOUND SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN .. .. KEYS (in order of precedence): ...... vector sequence Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, high end compl 1, ok 0 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_85 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG RIGHT PRIMER 57 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_86 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG RIGHT PRIMER 57 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00 TARGETS (start, len)*: 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>>* <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence ****** target >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_87 INPUT PROBLEM: Too many elements for tag SEQUENCE_TARGET PRIMER PICKING RESULTS FOR boundary_test_88 No mispriming library specified Using 1-based sequence positions OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 25 62.96 32.00 8.00 6.00 AATGTGATTGCATCAAATGTGATTG RIGHT PRIMER 57 25 61.17 32.00 5.00 5.00 GAATTGTGGAAATGACAATCACATT SEQUENCE SIZE: 59 INCLUDED REGION SIZE: 55 PRODUCT SIZE: 55, PAIR ANY COMPL: 11.00, PAIR 3' COMPL: 10.00 EXCLUDED REGIONS (start, len)*: 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 1 NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN ..>>>>>>>>>>>>>>>>>>>>>>>>>X <<<<<<<<<<<<<<<<<<<<<<<<<.. KEYS (in order of precedence): ...... vector sequence XXXXXX excluded region >>>>>> left primer <<<<<< right primer Statistics con too in in not no tm tm high high high sid many tar excl ok bad GC too too any 3' poly end ered Ns get reg reg GC% clamp low high compl compl X stab ok Left 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Right 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Pair Stats: considered 1, ok 1 libprimer3 release 2.2.1 PRIMER PICKING RESULTS FOR boundary_test_89 INPUT PROBLEM: Too many elements for tag SEQUENCE_EXCLUDED_REGION primer3-2.3.6/test/primer_high_tm_load_set_output0000664000077300007730000001174412231156245021256 0ustar ioanaioanaP3_SETTINGS_FILE_USED=../test/primer_high_tm_load_set.set P3_FILE_TYPE=settings P3_FILE_ID=Test with higher Tm PRIMER_MIN_TM=63 PRIMER_OPT_TM=65 PRIMER_MAX_TM=67 P3_SETTINGS_FILE_END= SEQUENCE_ID=Load qPCR settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_TASK=pick_detection_primers PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.107238 PRIMER_LEFT_0_PENALTY=0.030618 PRIMER_RIGHT_0_PENALTY=0.076620 PRIMER_LEFT_0_SEQUENCE=accgtgcaccggaatcagtt PRIMER_RIGHT_0_SEQUENCE=cggaaaaccagcctccaatg PRIMER_LEFT_0=1030,20 PRIMER_RIGHT_0=1205,20 PRIMER_LEFT_0_TM=65.031 PRIMER_RIGHT_0_TM=64.923 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=176 PRIMER_PAIR_1_PENALTY=0.168388 PRIMER_LEFT_1_PENALTY=0.051236 PRIMER_RIGHT_1_PENALTY=0.117152 PRIMER_LEFT_1_SEQUENCE=ggttttccgatgcagaaccg PRIMER_RIGHT_1_SEQUENCE=aaagctcaccggaactcccc PRIMER_LEFT_1=1197,20 PRIMER_RIGHT_1=1364,20 PRIMER_LEFT_1_TM=64.949 PRIMER_RIGHT_1_TM=64.883 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.9000 PRIMER_RIGHT_1_END_STABILITY=10.9000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=168 PRIMER_PAIR_2_PENALTY=0.191614 PRIMER_LEFT_2_PENALTY=0.124657 PRIMER_RIGHT_2_PENALTY=0.066957 PRIMER_LEFT_2_SEQUENCE=cacaaatggtccccacgaca PRIMER_RIGHT_2_SEQUENCE=gcctgccggtttcttgtacg PRIMER_LEFT_2=581,20 PRIMER_RIGHT_2=876,20 PRIMER_LEFT_2_TM=65.125 PRIMER_RIGHT_2_TM=65.067 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=7.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=296 PRIMER_PAIR_3_PENALTY=0.193772 PRIMER_LEFT_3_PENALTY=0.076620 PRIMER_RIGHT_3_PENALTY=0.117152 PRIMER_LEFT_3_SEQUENCE=cattggaggctggttttccg PRIMER_RIGHT_3_SEQUENCE=aaagctcaccggaactcccc PRIMER_LEFT_3=1186,20 PRIMER_RIGHT_3=1364,20 PRIMER_LEFT_3_TM=64.923 PRIMER_RIGHT_3_TM=64.883 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=10.2000 PRIMER_RIGHT_3_END_STABILITY=10.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=179 PRIMER_PAIR_4_PENALTY=0.197675 PRIMER_LEFT_4_PENALTY=0.124657 PRIMER_RIGHT_4_PENALTY=0.073018 PRIMER_LEFT_4_SEQUENCE=cacaaatggtccccacgaca PRIMER_RIGHT_4_SEQUENCE=cctgccggtttcttgtacgg PRIMER_LEFT_4=581,20 PRIMER_RIGHT_4=875,20 PRIMER_LEFT_4_TM=65.125 PRIMER_RIGHT_4_TM=64.927 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=8.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=295 = primer3-2.3.6/test/primer_must_use_output0000664000077300007730000005051712231156245017632 0ustar ioanaioanaSEQUENCE_ID=anyway_1 PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 PRIMER_PICK_ANYWAY=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, ok 3 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=high_tm PRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_PROBLEMS= Temperature too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=high_tm_and_self_any PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_PROBLEMS= Temperature too high; Similarity to self too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=high_tm_and_self_any_and_target PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=20,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 PRIMER_RIGHT_EXPLAIN=considered 3, high tm 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_PROBLEMS= Overlaps target; Temperature too high; Similarity to self too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=high_tm_and_self_any_and_excl_region PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 SEQUENCE_EXCLUDED_REGION=20,1 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_PROBLEMS= Temperature too high; Overlaps an excluded region; Similarity to self too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=high_tm_and_self_any_and_incl_region PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 SEQUENCE_INCLUDED_REGION=1,160 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG PRIMER_MAX_TM=60 PRIMER_ERROR=Specified left primer not in Included Region = SEQUENCE_ID=left_high_tm_and_self_any_and_right PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_PROBLEMS= Temperature too high; Similarity to self too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_PROBLEMS= Temperature too high; Similarity to self too high; PRIMER_RIGHT_0_PROBLEMS= GC content too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=left_high_tm_and_self_any_and_right_bad_gc_and_target PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_LEFT_0_PROBLEMS= Temperature too high; Similarity to self too high; PRIMER_RIGHT_0_PROBLEMS= Overlaps target; GC content too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=internal_too_short PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccat PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 PRIMER_WARNING=Specified internal oligo < PRIMER_INTERNAL_MIN_SIZE PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_INTERNAL_0_PENALTY=25.881506 PRIMER_LEFT_0_PROBLEMS= Temperature too high; Similarity to self too high; PRIMER_RIGHT_0_PROBLEMS= Overlaps target; GC content too high; PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; Too short; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_0_SEQUENCE=gcgtacgatccat PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_INTERNAL_0=23,13 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_INTERNAL_0_TM=41.118 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=53.846 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=internal_ok PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_INTERNAL_0_PENALTY=1.580164 PRIMER_LEFT_0_PROBLEMS= Temperature too high; Similarity to self too high; PRIMER_RIGHT_0_PROBLEMS= Overlaps target; GC content too high; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_0_SEQUENCE=gcgtacgatccatgctagc PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_INTERNAL_0=23,19 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_INTERNAL_0_TM=59.420 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=internal_too_cold PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 143,1 SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATG SEQUENCE_PRIMER_REVCOMP=CAGTGTGTACATGGCGGC SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=60 PRIMER_MAX_GC=60 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.432274 PRIMER_LEFT_0_PENALTY=1.117633 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_INTERNAL_0_PENALTY=1.580164 PRIMER_LEFT_0_PROBLEMS= Temperature too high; Similarity to self too high; PRIMER_RIGHT_0_PROBLEMS= Overlaps target; GC content too high; PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; PRIMER_LEFT_0_SEQUENCE=TCCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_0_SEQUENCE=gcgtacgatccatgctagc PRIMER_LEFT_0=0,21 PRIMER_RIGHT_0=160,18 PRIMER_INTERNAL_0=23,19 PRIMER_LEFT_0_TM=60.118 PRIMER_RIGHT_0_TM=59.685 PRIMER_INTERNAL_0_TM=59.420 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=161 = SEQUENCE_ID=internal_too_cold2 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=CCTACAGCTGTGGGAAAATG SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=100 PRIMER_MAX_GC=100 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.086374 PRIMER_LEFT_0_PENALTY=1.771733 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_INTERNAL_0_PENALTY=1.580164 PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; PRIMER_LEFT_0_SEQUENCE=CCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_0_SEQUENCE=gcgtacgatccatgctagc PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=160,18 PRIMER_INTERNAL_0=23,19 PRIMER_LEFT_0_TM=58.228 PRIMER_RIGHT_0_TM=59.685 PRIMER_INTERNAL_0_TM=59.420 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=10.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=160 = SEQUENCE_ID=mispriming PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_NUM_RETURN=1 SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 SEQUENCE_PRIMER=CCTACAGCTGTGGGAAAATG SEQUENCE_INTERNAL_OLIGO=gcgtacgatccatgctagc PRIMER_INTERNAL_MIN_TM=60 PRIMER_MAX_TM=100 PRIMER_MAX_GC=100 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_MAX_LIBRARY_MISPRIMING=8 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=1 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.086374 PRIMER_LEFT_0_PENALTY=1.771733 PRIMER_RIGHT_0_PENALTY=2.314641 PRIMER_INTERNAL_0_PENALTY=1.580164 PRIMER_LEFT_0_PROBLEMS= Similarity to non-template sequence too high; PRIMER_INTERNAL_0_PROBLEMS= Temperature too low; PRIMER_LEFT_0_SEQUENCE=CCTACAGCTGTGGGAAAATG PRIMER_RIGHT_0_SEQUENCE=CAGTGTGTACATGGCGGC PRIMER_INTERNAL_0_SEQUENCE=gcgtacgatccatgctagc PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=160,18 PRIMER_INTERNAL_0=23,19 PRIMER_LEFT_0_TM=58.228 PRIMER_RIGHT_0_TM=59.685 PRIMER_INTERNAL_0_TM=59.420 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=10.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=6.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=12.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=160 = primer3-2.3.6/test/primer_num_best_output0000664000077300007730000037050112231156245017600 0ustar ioanaioanaPRIMER_NUM_RETURN=2 PRIMER_OPT_SIZE=20 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000 PRIMER_MAX_GC=80.0 PRIMER_MAX_SIZE=25 PRIMER_MAX_TM=63.0 PRIMER_MIN_SIZE=18 PRIMER_MIN_GC=20 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=6 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=6 SEQUENCE_ID=01.RHAP33FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,315 SEQUENCE_TARGET=81,30 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=7.733854 PRIMER_LEFT_0_PENALTY=7.538496 PRIMER_RIGHT_0_PENALTY=0.195358 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_0_SEQUENCE=GGCAAAAGGACAGAGGATGA PRIMER_LEFT_0=20,25 PRIMER_RIGHT_0=177,20 PRIMER_LEFT_0_TM=57.462 PRIMER_RIGHT_0_TM=60.195 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.0000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=158 PRIMER_PAIR_1_PENALTY=7.868046 PRIMER_LEFT_1_PENALTY=7.538496 PRIMER_RIGHT_1_PENALTY=0.329550 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_1_SEQUENCE=ATCTTGGGCAAAAGGACAGA PRIMER_LEFT_1=20,25 PRIMER_RIGHT_1=183,20 PRIMER_LEFT_1_TM=57.462 PRIMER_RIGHT_1_TM=59.670 PRIMER_LEFT_1_GC_PERCENT=32.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.0000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=164 = SEQUENCE_ID=01.RHAP34FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT SEQUENCE_INCLUDED_REGION=19,296 SEQUENCE_TARGET=134,15 SEQUENCE_TARGET=213,16 SEQUENCE_TARGET=235,16 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = PRIMER_NUM_RETURN=1 SEQUENCE_ID=03.RHAP34FA3.seq SEQUENCE_TEMPLATE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT SEQUENCE_INCLUDED_REGION=0,278 SEQUENCE_TARGET=1,28 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=03.RHAP34FE3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA SEQUENCE_INCLUDED_REGION=17,284 SEQUENCE_TARGET=27,60 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=04.RHAP33FE4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC SEQUENCE_INCLUDED_REGION=18,367 SEQUENCE_TARGET=146,46 SEQUENCE_TARGET=199,22 SEQUENCE_TARGET=286,13 SEQUENCE_TARGET=300,15 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=7.353468 PRIMER_LEFT_0_PENALTY=4.174047 PRIMER_RIGHT_0_PENALTY=3.179421 PRIMER_LEFT_0_SEQUENCE=TGAGAAGGGAGGAGTAAGTACTGG PRIMER_RIGHT_0_SEQUENCE=CCTATGCCTATACACCACACACA PRIMER_LEFT_0=97,24 PRIMER_RIGHT_0=270,23 PRIMER_LEFT_0_TM=60.174 PRIMER_RIGHT_0_TM=59.821 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=47.826 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 = SEQUENCE_ID=05.RHAP33FA5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA SEQUENCE_INCLUDED_REGION=10,369 SEQUENCE_TARGET=30,44 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=05.RHAP33FE5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,306 SEQUENCE_TARGET=176,34 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.858517 PRIMER_LEFT_0_PENALTY=0.702456 PRIMER_RIGHT_0_PENALTY=0.156061 PRIMER_LEFT_0_SEQUENCE=CATGTTCGTGCAAACACTCA PRIMER_RIGHT_0_SEQUENCE=AGACCCTGTCTCCAAAAGCA PRIMER_LEFT_0=129,20 PRIMER_RIGHT_0=302,20 PRIMER_LEFT_0_TM=59.298 PRIMER_RIGHT_0_TM=59.844 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 = PRIMER_NUM_RETURN=40 SEQUENCE_ID=05.RHAP34FA5.seq SEQUENCE_TEMPLATE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA SEQUENCE_INCLUDED_REGION=9,297 SEQUENCE_TARGET=125,36 SEQUENCE_TARGET=162,24 SEQUENCE_TARGET=191,16 SEQUENCE_TARGET=214,14 PRIMER_LEFT_NUM_RETURNED=40 PRIMER_RIGHT_NUM_RETURNED=40 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=40 PRIMER_PAIR_0_PENALTY=9.884342 PRIMER_LEFT_0_PENALTY=1.973952 PRIMER_RIGHT_0_PENALTY=7.910390 PRIMER_LEFT_0_SEQUENCE=GCGGCCGACATCTACTACTA PRIMER_RIGHT_0_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_0=103,20 PRIMER_RIGHT_0=268,25 PRIMER_LEFT_0_TM=58.026 PRIMER_RIGHT_0_TM=57.090 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=44.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=4.7000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=166 PRIMER_PAIR_1_PENALTY=10.012256 PRIMER_LEFT_1_PENALTY=2.101866 PRIMER_RIGHT_1_PENALTY=7.910390 PRIMER_LEFT_1_SEQUENCE=GCGGCCGACATCTACTACTAC PRIMER_RIGHT_1_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_1=103,21 PRIMER_RIGHT_1=268,25 PRIMER_LEFT_1_TM=58.898 PRIMER_RIGHT_1_TM=57.090 PRIMER_LEFT_1_GC_PERCENT=57.143 PRIMER_RIGHT_1_GC_PERCENT=44.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=5.1000 PRIMER_RIGHT_1_END_STABILITY=6.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=166 PRIMER_PAIR_2_PENALTY=10.079221 PRIMER_LEFT_2_PENALTY=2.168831 PRIMER_RIGHT_2_PENALTY=7.910390 PRIMER_LEFT_2_SEQUENCE=GCGGCCGACATCTACTACTACT PRIMER_RIGHT_2_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_2=103,22 PRIMER_RIGHT_2=268,25 PRIMER_LEFT_2_TM=59.831 PRIMER_RIGHT_2_TM=57.090 PRIMER_LEFT_2_GC_PERCENT=54.545 PRIMER_RIGHT_2_GC_PERCENT=44.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=5.4000 PRIMER_RIGHT_2_END_STABILITY=6.0000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=166 PRIMER_PAIR_3_PENALTY=10.514211 PRIMER_LEFT_3_PENALTY=2.603822 PRIMER_RIGHT_3_PENALTY=7.910390 PRIMER_LEFT_3_SEQUENCE=CGCGGCCGACATCTACTACT PRIMER_RIGHT_3_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_3=102,20 PRIMER_RIGHT_3=268,25 PRIMER_LEFT_3_TM=62.604 PRIMER_RIGHT_3_TM=57.090 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=44.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=5.4000 PRIMER_RIGHT_3_END_STABILITY=6.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=167 PRIMER_PAIR_4_PENALTY=10.656616 PRIMER_LEFT_4_PENALTY=2.746227 PRIMER_RIGHT_4_PENALTY=7.910390 PRIMER_LEFT_4_SEQUENCE=CGCGGCCGACATCTACTAC PRIMER_RIGHT_4_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_4=102,19 PRIMER_RIGHT_4=268,25 PRIMER_LEFT_4_TM=61.746 PRIMER_RIGHT_4_TM=57.090 PRIMER_LEFT_4_GC_PERCENT=63.158 PRIMER_RIGHT_4_GC_PERCENT=44.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=5.1000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=167 PRIMER_PAIR_5_PENALTY=10.846735 PRIMER_LEFT_5_PENALTY=2.936346 PRIMER_RIGHT_5_PENALTY=7.910390 PRIMER_LEFT_5_SEQUENCE=CGCGGCCGACATCTACTA PRIMER_RIGHT_5_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_5=102,18 PRIMER_RIGHT_5=268,25 PRIMER_LEFT_5_TM=60.936 PRIMER_RIGHT_5_TM=57.090 PRIMER_LEFT_5_GC_PERCENT=61.111 PRIMER_RIGHT_5_GC_PERCENT=44.000 PRIMER_LEFT_5_SELF_ANY=6.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_END_STABILITY=4.7000 PRIMER_RIGHT_5_END_STABILITY=6.0000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PAIR_5_PRODUCT_SIZE=167 PRIMER_PAIR_6_PENALTY=10.974908 PRIMER_LEFT_6_PENALTY=3.064519 PRIMER_RIGHT_6_PENALTY=7.910390 PRIMER_LEFT_6_SEQUENCE=GCGGCCGACATCTACTACT PRIMER_RIGHT_6_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_6=103,19 PRIMER_RIGHT_6=268,25 PRIMER_LEFT_6_TM=57.935 PRIMER_RIGHT_6_TM=57.090 PRIMER_LEFT_6_GC_PERCENT=57.895 PRIMER_RIGHT_6_GC_PERCENT=44.000 PRIMER_LEFT_6_SELF_ANY=6.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_END_STABILITY=5.4000 PRIMER_RIGHT_6_END_STABILITY=6.0000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PAIR_6_PRODUCT_SIZE=166 PRIMER_PAIR_7_PENALTY=11.429982 PRIMER_LEFT_7_PENALTY=3.519592 PRIMER_RIGHT_7_PENALTY=7.910390 PRIMER_LEFT_7_SEQUENCE=CGCGGCCGACATCTACTACTA PRIMER_RIGHT_7_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_7=102,21 PRIMER_RIGHT_7=268,25 PRIMER_LEFT_7_TM=62.520 PRIMER_RIGHT_7_TM=57.090 PRIMER_LEFT_7_GC_PERCENT=57.143 PRIMER_RIGHT_7_GC_PERCENT=44.000 PRIMER_LEFT_7_SELF_ANY=6.00 PRIMER_RIGHT_7_SELF_ANY=3.00 PRIMER_LEFT_7_SELF_END=4.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_END_STABILITY=4.7000 PRIMER_RIGHT_7_END_STABILITY=6.0000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PAIR_7_PRODUCT_SIZE=167 PRIMER_PAIR_8_PENALTY=0.347871 PRIMER_LEFT_8_PENALTY=0.003322 PRIMER_RIGHT_8_PENALTY=0.344549 PRIMER_LEFT_8_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_8_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_8=54,20 PRIMER_RIGHT_8=294,20 PRIMER_LEFT_8_TM=59.997 PRIMER_RIGHT_8_TM=60.345 PRIMER_LEFT_8_GC_PERCENT=55.000 PRIMER_RIGHT_8_GC_PERCENT=50.000 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=7.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_LEFT_8_END_STABILITY=9.4000 PRIMER_RIGHT_8_END_STABILITY=8.2000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PAIR_8_PRODUCT_SIZE=241 PRIMER_PAIR_9_PENALTY=0.387281 PRIMER_LEFT_9_PENALTY=0.042732 PRIMER_RIGHT_9_PENALTY=0.344549 PRIMER_LEFT_9_SEQUENCE=GAAGGCCACTCTCATTCAGC PRIMER_RIGHT_9_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_9=65,20 PRIMER_RIGHT_9=294,20 PRIMER_LEFT_9_TM=59.957 PRIMER_RIGHT_9_TM=60.345 PRIMER_LEFT_9_GC_PERCENT=55.000 PRIMER_RIGHT_9_GC_PERCENT=50.000 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=7.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=0.00 PRIMER_LEFT_9_END_STABILITY=8.2000 PRIMER_RIGHT_9_END_STABILITY=8.2000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PAIR_9_PRODUCT_SIZE=230 PRIMER_PAIR_10_PENALTY=0.478290 PRIMER_LEFT_10_PENALTY=0.133741 PRIMER_RIGHT_10_PENALTY=0.344549 PRIMER_LEFT_10_SEQUENCE=AGCTGAGACTTTGAAGGCCA PRIMER_RIGHT_10_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_10=53,20 PRIMER_RIGHT_10=294,20 PRIMER_LEFT_10_TM=60.134 PRIMER_RIGHT_10_TM=60.345 PRIMER_LEFT_10_GC_PERCENT=50.000 PRIMER_RIGHT_10_GC_PERCENT=50.000 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=7.00 PRIMER_LEFT_10_SELF_END=4.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_END_STABILITY=11.2000 PRIMER_RIGHT_10_END_STABILITY=8.2000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=242 PRIMER_PAIR_11_PENALTY=0.478290 PRIMER_LEFT_11_PENALTY=0.133741 PRIMER_RIGHT_11_PENALTY=0.344549 PRIMER_LEFT_11_SEQUENCE=CAGCTGAGACTTTGAAGGCC PRIMER_RIGHT_11_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_11=52,20 PRIMER_RIGHT_11=294,20 PRIMER_LEFT_11_TM=60.134 PRIMER_RIGHT_11_TM=60.345 PRIMER_LEFT_11_GC_PERCENT=55.000 PRIMER_RIGHT_11_GC_PERCENT=50.000 PRIMER_LEFT_11_SELF_ANY=6.00 PRIMER_RIGHT_11_SELF_ANY=7.00 PRIMER_LEFT_11_SELF_END=4.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_END_STABILITY=10.9000 PRIMER_RIGHT_11_END_STABILITY=8.2000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PAIR_11_PRODUCT_SIZE=243 PRIMER_PAIR_12_PENALTY=0.605306 PRIMER_LEFT_12_PENALTY=0.260757 PRIMER_RIGHT_12_PENALTY=0.344549 PRIMER_LEFT_12_SEQUENCE=CCACTCTCATTCAGCCAGGT PRIMER_RIGHT_12_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_12=70,20 PRIMER_RIGHT_12=294,20 PRIMER_LEFT_12_TM=60.261 PRIMER_RIGHT_12_TM=60.345 PRIMER_LEFT_12_GC_PERCENT=55.000 PRIMER_RIGHT_12_GC_PERCENT=50.000 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=7.00 PRIMER_LEFT_12_SELF_END=1.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_END_STABILITY=7.9000 PRIMER_RIGHT_12_END_STABILITY=8.2000 PRIMER_PAIR_12_COMPL_ANY=3.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PAIR_12_PRODUCT_SIZE=225 PRIMER_PAIR_13_PENALTY=0.629670 PRIMER_LEFT_13_PENALTY=0.285121 PRIMER_RIGHT_13_PENALTY=0.344549 PRIMER_LEFT_13_SEQUENCE=GCAGCTGAGACTTTGAAGGC PRIMER_RIGHT_13_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_13=51,20 PRIMER_RIGHT_13=294,20 PRIMER_LEFT_13_TM=60.285 PRIMER_RIGHT_13_TM=60.345 PRIMER_LEFT_13_GC_PERCENT=55.000 PRIMER_RIGHT_13_GC_PERCENT=50.000 PRIMER_LEFT_13_SELF_ANY=6.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_LEFT_13_SELF_END=4.00 PRIMER_RIGHT_13_SELF_END=0.00 PRIMER_LEFT_13_END_STABILITY=9.7000 PRIMER_RIGHT_13_END_STABILITY=8.2000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PAIR_13_PRODUCT_SIZE=244 PRIMER_PAIR_14_PENALTY=0.653951 PRIMER_LEFT_14_PENALTY=0.309402 PRIMER_RIGHT_14_PENALTY=0.344549 PRIMER_LEFT_14_SEQUENCE=CATTCAGCCAGGTGTGTGAG PRIMER_RIGHT_14_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_14=77,20 PRIMER_RIGHT_14=294,20 PRIMER_LEFT_14_TM=60.309 PRIMER_RIGHT_14_TM=60.345 PRIMER_LEFT_14_GC_PERCENT=55.000 PRIMER_RIGHT_14_GC_PERCENT=50.000 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=7.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_LEFT_14_END_STABILITY=6.4000 PRIMER_RIGHT_14_END_STABILITY=8.2000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=4.00 PRIMER_PAIR_14_PRODUCT_SIZE=218 PRIMER_PAIR_15_PENALTY=0.653951 PRIMER_LEFT_15_PENALTY=0.309402 PRIMER_RIGHT_15_PENALTY=0.344549 PRIMER_LEFT_15_SEQUENCE=CTCATTCAGCCAGGTGTGTG PRIMER_RIGHT_15_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_15=75,20 PRIMER_RIGHT_15=294,20 PRIMER_LEFT_15_TM=60.309 PRIMER_RIGHT_15_TM=60.345 PRIMER_LEFT_15_GC_PERCENT=55.000 PRIMER_RIGHT_15_GC_PERCENT=50.000 PRIMER_LEFT_15_SELF_ANY=3.00 PRIMER_RIGHT_15_SELF_ANY=7.00 PRIMER_LEFT_15_SELF_END=3.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_END_STABILITY=6.4000 PRIMER_RIGHT_15_END_STABILITY=8.2000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=4.00 PRIMER_PAIR_15_PRODUCT_SIZE=220 PRIMER_PAIR_16_PENALTY=0.929938 PRIMER_LEFT_16_PENALTY=0.585389 PRIMER_RIGHT_16_PENALTY=0.344549 PRIMER_LEFT_16_SEQUENCE=CTCTCATTCAGCCAGGTGTG PRIMER_RIGHT_16_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_16=73,20 PRIMER_RIGHT_16=294,20 PRIMER_LEFT_16_TM=59.415 PRIMER_RIGHT_16_TM=60.345 PRIMER_LEFT_16_GC_PERCENT=55.000 PRIMER_RIGHT_16_GC_PERCENT=50.000 PRIMER_LEFT_16_SELF_ANY=3.00 PRIMER_RIGHT_16_SELF_ANY=7.00 PRIMER_LEFT_16_SELF_END=1.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_END_STABILITY=6.4000 PRIMER_RIGHT_16_END_STABILITY=8.2000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=3.00 PRIMER_PAIR_16_PRODUCT_SIZE=222 PRIMER_PAIR_17_PENALTY=0.929938 PRIMER_LEFT_17_PENALTY=0.585389 PRIMER_RIGHT_17_PENALTY=0.344549 PRIMER_LEFT_17_SEQUENCE=CACTCTCATTCAGCCAGGTG PRIMER_RIGHT_17_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_17=71,20 PRIMER_RIGHT_17=294,20 PRIMER_LEFT_17_TM=59.415 PRIMER_RIGHT_17_TM=60.345 PRIMER_LEFT_17_GC_PERCENT=55.000 PRIMER_RIGHT_17_GC_PERCENT=50.000 PRIMER_LEFT_17_SELF_ANY=3.00 PRIMER_RIGHT_17_SELF_ANY=7.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_END_STABILITY=7.9000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PAIR_17_PRODUCT_SIZE=224 PRIMER_PAIR_18_PENALTY=0.975760 PRIMER_LEFT_18_PENALTY=0.631211 PRIMER_RIGHT_18_PENALTY=0.344549 PRIMER_LEFT_18_SEQUENCE=TTGAAGGCCACTCTCATTCA PRIMER_RIGHT_18_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_18=63,20 PRIMER_RIGHT_18=294,20 PRIMER_LEFT_18_TM=59.369 PRIMER_RIGHT_18_TM=60.345 PRIMER_LEFT_18_GC_PERCENT=45.000 PRIMER_RIGHT_18_GC_PERCENT=50.000 PRIMER_LEFT_18_SELF_ANY=5.00 PRIMER_RIGHT_18_SELF_ANY=7.00 PRIMER_LEFT_18_SELF_END=5.00 PRIMER_RIGHT_18_SELF_END=0.00 PRIMER_LEFT_18_END_STABILITY=6.9000 PRIMER_RIGHT_18_END_STABILITY=8.2000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PAIR_18_PRODUCT_SIZE=232 PRIMER_PAIR_19_PENALTY=1.030805 PRIMER_LEFT_19_PENALTY=0.686256 PRIMER_RIGHT_19_PENALTY=0.344549 PRIMER_LEFT_19_SEQUENCE=TGCAGCTGAGACTTTGAAGG PRIMER_RIGHT_19_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_19=50,20 PRIMER_RIGHT_19=294,20 PRIMER_LEFT_19_TM=59.314 PRIMER_RIGHT_19_TM=60.345 PRIMER_LEFT_19_GC_PERCENT=50.000 PRIMER_RIGHT_19_GC_PERCENT=50.000 PRIMER_LEFT_19_SELF_ANY=6.00 PRIMER_RIGHT_19_SELF_ANY=7.00 PRIMER_LEFT_19_SELF_END=3.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_END_STABILITY=8.2000 PRIMER_RIGHT_19_END_STABILITY=8.2000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=2.00 PRIMER_PAIR_19_PRODUCT_SIZE=245 PRIMER_PAIR_20_PENALTY=1.083301 PRIMER_LEFT_20_PENALTY=0.738752 PRIMER_RIGHT_20_PENALTY=0.344549 PRIMER_LEFT_20_SEQUENCE=TCTCATTCAGCCAGGTGTGT PRIMER_RIGHT_20_SEQUENCE=TAAGGTCCTTCCCACAACCA PRIMER_LEFT_20=74,20 PRIMER_RIGHT_20=294,20 PRIMER_LEFT_20_TM=59.261 PRIMER_RIGHT_20_TM=60.345 PRIMER_LEFT_20_GC_PERCENT=50.000 PRIMER_RIGHT_20_GC_PERCENT=50.000 PRIMER_LEFT_20_SELF_ANY=3.00 PRIMER_RIGHT_20_SELF_ANY=7.00 PRIMER_LEFT_20_SELF_END=1.00 PRIMER_RIGHT_20_SELF_END=0.00 PRIMER_LEFT_20_END_STABILITY=6.4000 PRIMER_RIGHT_20_END_STABILITY=8.2000 PRIMER_PAIR_20_COMPL_ANY=4.00 PRIMER_PAIR_20_COMPL_END=3.00 PRIMER_PAIR_20_PRODUCT_SIZE=221 PRIMER_PAIR_21_PENALTY=1.183339 PRIMER_LEFT_21_PENALTY=0.003322 PRIMER_RIGHT_21_PENALTY=1.180017 PRIMER_LEFT_21_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_21_SEQUENCE=GTCCTTCCCACAACCAACCT PRIMER_LEFT_21=54,20 PRIMER_RIGHT_21=290,20 PRIMER_LEFT_21_TM=59.997 PRIMER_RIGHT_21_TM=61.180 PRIMER_LEFT_21_GC_PERCENT=55.000 PRIMER_RIGHT_21_GC_PERCENT=55.000 PRIMER_LEFT_21_SELF_ANY=4.00 PRIMER_RIGHT_21_SELF_ANY=2.00 PRIMER_LEFT_21_SELF_END=3.00 PRIMER_RIGHT_21_SELF_END=0.00 PRIMER_LEFT_21_END_STABILITY=9.4000 PRIMER_RIGHT_21_END_STABILITY=7.9000 PRIMER_PAIR_21_COMPL_ANY=5.00 PRIMER_PAIR_21_COMPL_END=0.00 PRIMER_PAIR_21_PRODUCT_SIZE=237 PRIMER_PAIR_22_PENALTY=1.183339 PRIMER_LEFT_22_PENALTY=0.003322 PRIMER_RIGHT_22_PENALTY=1.180017 PRIMER_LEFT_22_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_22_SEQUENCE=AGGTCCTTCCCACAACCAAC PRIMER_LEFT_22=54,20 PRIMER_RIGHT_22=292,20 PRIMER_LEFT_22_TM=59.997 PRIMER_RIGHT_22_TM=61.180 PRIMER_LEFT_22_GC_PERCENT=55.000 PRIMER_RIGHT_22_GC_PERCENT=55.000 PRIMER_LEFT_22_SELF_ANY=4.00 PRIMER_RIGHT_22_SELF_ANY=5.00 PRIMER_LEFT_22_SELF_END=3.00 PRIMER_RIGHT_22_SELF_END=0.00 PRIMER_LEFT_22_END_STABILITY=9.4000 PRIMER_RIGHT_22_END_STABILITY=8.2000 PRIMER_PAIR_22_COMPL_ANY=5.00 PRIMER_PAIR_22_COMPL_END=1.00 PRIMER_PAIR_22_PRODUCT_SIZE=239 PRIMER_PAIR_23_PENALTY=1.210181 PRIMER_LEFT_23_PENALTY=0.003322 PRIMER_RIGHT_23_PENALTY=1.206859 PRIMER_LEFT_23_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_23_SEQUENCE=GTCCTTCCCACAACCAACC PRIMER_LEFT_23=54,20 PRIMER_RIGHT_23=290,19 PRIMER_LEFT_23_TM=59.997 PRIMER_RIGHT_23_TM=60.207 PRIMER_LEFT_23_GC_PERCENT=55.000 PRIMER_RIGHT_23_GC_PERCENT=57.895 PRIMER_LEFT_23_SELF_ANY=4.00 PRIMER_RIGHT_23_SELF_ANY=2.00 PRIMER_LEFT_23_SELF_END=3.00 PRIMER_RIGHT_23_SELF_END=0.00 PRIMER_LEFT_23_END_STABILITY=9.4000 PRIMER_RIGHT_23_END_STABILITY=8.2000 PRIMER_PAIR_23_COMPL_ANY=5.00 PRIMER_PAIR_23_COMPL_END=0.00 PRIMER_PAIR_23_PRODUCT_SIZE=237 PRIMER_PAIR_24_PENALTY=1.210181 PRIMER_LEFT_24_PENALTY=0.003322 PRIMER_RIGHT_24_PENALTY=1.206859 PRIMER_LEFT_24_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_24_SEQUENCE=GGTCCTTCCCACAACCAAC PRIMER_LEFT_24=54,20 PRIMER_RIGHT_24=291,19 PRIMER_LEFT_24_TM=59.997 PRIMER_RIGHT_24_TM=60.207 PRIMER_LEFT_24_GC_PERCENT=55.000 PRIMER_RIGHT_24_GC_PERCENT=57.895 PRIMER_LEFT_24_SELF_ANY=4.00 PRIMER_RIGHT_24_SELF_ANY=3.00 PRIMER_LEFT_24_SELF_END=3.00 PRIMER_RIGHT_24_SELF_END=0.00 PRIMER_LEFT_24_END_STABILITY=9.4000 PRIMER_RIGHT_24_END_STABILITY=8.2000 PRIMER_PAIR_24_COMPL_ANY=5.00 PRIMER_PAIR_24_COMPL_END=2.00 PRIMER_PAIR_24_PRODUCT_SIZE=238 PRIMER_PAIR_25_PENALTY=1.222749 PRIMER_LEFT_25_PENALTY=0.042732 PRIMER_RIGHT_25_PENALTY=1.180017 PRIMER_LEFT_25_SEQUENCE=GAAGGCCACTCTCATTCAGC PRIMER_RIGHT_25_SEQUENCE=GTCCTTCCCACAACCAACCT PRIMER_LEFT_25=65,20 PRIMER_RIGHT_25=290,20 PRIMER_LEFT_25_TM=59.957 PRIMER_RIGHT_25_TM=61.180 PRIMER_LEFT_25_GC_PERCENT=55.000 PRIMER_RIGHT_25_GC_PERCENT=55.000 PRIMER_LEFT_25_SELF_ANY=4.00 PRIMER_RIGHT_25_SELF_ANY=2.00 PRIMER_LEFT_25_SELF_END=2.00 PRIMER_RIGHT_25_SELF_END=0.00 PRIMER_LEFT_25_END_STABILITY=8.2000 PRIMER_RIGHT_25_END_STABILITY=7.9000 PRIMER_PAIR_25_COMPL_ANY=5.00 PRIMER_PAIR_25_COMPL_END=1.00 PRIMER_PAIR_25_PRODUCT_SIZE=226 PRIMER_PAIR_26_PENALTY=1.222749 PRIMER_LEFT_26_PENALTY=0.042732 PRIMER_RIGHT_26_PENALTY=1.180017 PRIMER_LEFT_26_SEQUENCE=GAAGGCCACTCTCATTCAGC PRIMER_RIGHT_26_SEQUENCE=AGGTCCTTCCCACAACCAAC PRIMER_LEFT_26=65,20 PRIMER_RIGHT_26=292,20 PRIMER_LEFT_26_TM=59.957 PRIMER_RIGHT_26_TM=61.180 PRIMER_LEFT_26_GC_PERCENT=55.000 PRIMER_RIGHT_26_GC_PERCENT=55.000 PRIMER_LEFT_26_SELF_ANY=4.00 PRIMER_RIGHT_26_SELF_ANY=5.00 PRIMER_LEFT_26_SELF_END=2.00 PRIMER_RIGHT_26_SELF_END=0.00 PRIMER_LEFT_26_END_STABILITY=8.2000 PRIMER_RIGHT_26_END_STABILITY=8.2000 PRIMER_PAIR_26_COMPL_ANY=5.00 PRIMER_PAIR_26_COMPL_END=0.00 PRIMER_PAIR_26_PRODUCT_SIZE=228 PRIMER_PAIR_27_PENALTY=1.249591 PRIMER_LEFT_27_PENALTY=0.042732 PRIMER_RIGHT_27_PENALTY=1.206859 PRIMER_LEFT_27_SEQUENCE=GAAGGCCACTCTCATTCAGC PRIMER_RIGHT_27_SEQUENCE=GTCCTTCCCACAACCAACC PRIMER_LEFT_27=65,20 PRIMER_RIGHT_27=290,19 PRIMER_LEFT_27_TM=59.957 PRIMER_RIGHT_27_TM=60.207 PRIMER_LEFT_27_GC_PERCENT=55.000 PRIMER_RIGHT_27_GC_PERCENT=57.895 PRIMER_LEFT_27_SELF_ANY=4.00 PRIMER_RIGHT_27_SELF_ANY=2.00 PRIMER_LEFT_27_SELF_END=2.00 PRIMER_RIGHT_27_SELF_END=0.00 PRIMER_LEFT_27_END_STABILITY=8.2000 PRIMER_RIGHT_27_END_STABILITY=8.2000 PRIMER_PAIR_27_COMPL_ANY=5.00 PRIMER_PAIR_27_COMPL_END=0.00 PRIMER_PAIR_27_PRODUCT_SIZE=226 PRIMER_PAIR_28_PENALTY=1.249591 PRIMER_LEFT_28_PENALTY=0.042732 PRIMER_RIGHT_28_PENALTY=1.206859 PRIMER_LEFT_28_SEQUENCE=GAAGGCCACTCTCATTCAGC PRIMER_RIGHT_28_SEQUENCE=GGTCCTTCCCACAACCAAC PRIMER_LEFT_28=65,20 PRIMER_RIGHT_28=291,19 PRIMER_LEFT_28_TM=59.957 PRIMER_RIGHT_28_TM=60.207 PRIMER_LEFT_28_GC_PERCENT=55.000 PRIMER_RIGHT_28_GC_PERCENT=57.895 PRIMER_LEFT_28_SELF_ANY=4.00 PRIMER_RIGHT_28_SELF_ANY=3.00 PRIMER_LEFT_28_SELF_END=2.00 PRIMER_RIGHT_28_SELF_END=0.00 PRIMER_LEFT_28_END_STABILITY=8.2000 PRIMER_RIGHT_28_END_STABILITY=8.2000 PRIMER_PAIR_28_COMPL_ANY=5.00 PRIMER_PAIR_28_COMPL_END=0.00 PRIMER_PAIR_28_PRODUCT_SIZE=227 PRIMER_PAIR_29_PENALTY=1.313757 PRIMER_LEFT_29_PENALTY=0.133741 PRIMER_RIGHT_29_PENALTY=1.180017 PRIMER_LEFT_29_SEQUENCE=AGCTGAGACTTTGAAGGCCA PRIMER_RIGHT_29_SEQUENCE=GTCCTTCCCACAACCAACCT PRIMER_LEFT_29=53,20 PRIMER_RIGHT_29=290,20 PRIMER_LEFT_29_TM=60.134 PRIMER_RIGHT_29_TM=61.180 PRIMER_LEFT_29_GC_PERCENT=50.000 PRIMER_RIGHT_29_GC_PERCENT=55.000 PRIMER_LEFT_29_SELF_ANY=4.00 PRIMER_RIGHT_29_SELF_ANY=2.00 PRIMER_LEFT_29_SELF_END=4.00 PRIMER_RIGHT_29_SELF_END=0.00 PRIMER_LEFT_29_END_STABILITY=11.2000 PRIMER_RIGHT_29_END_STABILITY=7.9000 PRIMER_PAIR_29_COMPL_ANY=5.00 PRIMER_PAIR_29_COMPL_END=1.00 PRIMER_PAIR_29_PRODUCT_SIZE=238 PRIMER_PAIR_30_PENALTY=1.313757 PRIMER_LEFT_30_PENALTY=0.133741 PRIMER_RIGHT_30_PENALTY=1.180017 PRIMER_LEFT_30_SEQUENCE=AGCTGAGACTTTGAAGGCCA PRIMER_RIGHT_30_SEQUENCE=AGGTCCTTCCCACAACCAAC PRIMER_LEFT_30=53,20 PRIMER_RIGHT_30=292,20 PRIMER_LEFT_30_TM=60.134 PRIMER_RIGHT_30_TM=61.180 PRIMER_LEFT_30_GC_PERCENT=50.000 PRIMER_RIGHT_30_GC_PERCENT=55.000 PRIMER_LEFT_30_SELF_ANY=4.00 PRIMER_RIGHT_30_SELF_ANY=5.00 PRIMER_LEFT_30_SELF_END=4.00 PRIMER_RIGHT_30_SELF_END=0.00 PRIMER_LEFT_30_END_STABILITY=11.2000 PRIMER_RIGHT_30_END_STABILITY=8.2000 PRIMER_PAIR_30_COMPL_ANY=5.00 PRIMER_PAIR_30_COMPL_END=1.00 PRIMER_PAIR_30_PRODUCT_SIZE=240 PRIMER_PAIR_31_PENALTY=1.313757 PRIMER_LEFT_31_PENALTY=0.133741 PRIMER_RIGHT_31_PENALTY=1.180017 PRIMER_LEFT_31_SEQUENCE=CAGCTGAGACTTTGAAGGCC PRIMER_RIGHT_31_SEQUENCE=GTCCTTCCCACAACCAACCT PRIMER_LEFT_31=52,20 PRIMER_RIGHT_31=290,20 PRIMER_LEFT_31_TM=60.134 PRIMER_RIGHT_31_TM=61.180 PRIMER_LEFT_31_GC_PERCENT=55.000 PRIMER_RIGHT_31_GC_PERCENT=55.000 PRIMER_LEFT_31_SELF_ANY=6.00 PRIMER_RIGHT_31_SELF_ANY=2.00 PRIMER_LEFT_31_SELF_END=4.00 PRIMER_RIGHT_31_SELF_END=0.00 PRIMER_LEFT_31_END_STABILITY=10.9000 PRIMER_RIGHT_31_END_STABILITY=7.9000 PRIMER_PAIR_31_COMPL_ANY=5.00 PRIMER_PAIR_31_COMPL_END=1.00 PRIMER_PAIR_31_PRODUCT_SIZE=239 PRIMER_PAIR_32_PENALTY=1.313757 PRIMER_LEFT_32_PENALTY=0.133741 PRIMER_RIGHT_32_PENALTY=1.180017 PRIMER_LEFT_32_SEQUENCE=CAGCTGAGACTTTGAAGGCC PRIMER_RIGHT_32_SEQUENCE=AGGTCCTTCCCACAACCAAC PRIMER_LEFT_32=52,20 PRIMER_RIGHT_32=292,20 PRIMER_LEFT_32_TM=60.134 PRIMER_RIGHT_32_TM=61.180 PRIMER_LEFT_32_GC_PERCENT=55.000 PRIMER_RIGHT_32_GC_PERCENT=55.000 PRIMER_LEFT_32_SELF_ANY=6.00 PRIMER_RIGHT_32_SELF_ANY=5.00 PRIMER_LEFT_32_SELF_END=4.00 PRIMER_RIGHT_32_SELF_END=0.00 PRIMER_LEFT_32_END_STABILITY=10.9000 PRIMER_RIGHT_32_END_STABILITY=8.2000 PRIMER_PAIR_32_COMPL_ANY=5.00 PRIMER_PAIR_32_COMPL_END=2.00 PRIMER_PAIR_32_PRODUCT_SIZE=241 PRIMER_PAIR_33_PENALTY=1.340600 PRIMER_LEFT_33_PENALTY=0.133741 PRIMER_RIGHT_33_PENALTY=1.206859 PRIMER_LEFT_33_SEQUENCE=AGCTGAGACTTTGAAGGCCA PRIMER_RIGHT_33_SEQUENCE=GTCCTTCCCACAACCAACC PRIMER_LEFT_33=53,20 PRIMER_RIGHT_33=290,19 PRIMER_LEFT_33_TM=60.134 PRIMER_RIGHT_33_TM=60.207 PRIMER_LEFT_33_GC_PERCENT=50.000 PRIMER_RIGHT_33_GC_PERCENT=57.895 PRIMER_LEFT_33_SELF_ANY=4.00 PRIMER_RIGHT_33_SELF_ANY=2.00 PRIMER_LEFT_33_SELF_END=4.00 PRIMER_RIGHT_33_SELF_END=0.00 PRIMER_LEFT_33_END_STABILITY=11.2000 PRIMER_RIGHT_33_END_STABILITY=8.2000 PRIMER_PAIR_33_COMPL_ANY=5.00 PRIMER_PAIR_33_COMPL_END=1.00 PRIMER_PAIR_33_PRODUCT_SIZE=238 PRIMER_PAIR_34_PENALTY=1.340600 PRIMER_LEFT_34_PENALTY=0.133741 PRIMER_RIGHT_34_PENALTY=1.206859 PRIMER_LEFT_34_SEQUENCE=AGCTGAGACTTTGAAGGCCA PRIMER_RIGHT_34_SEQUENCE=GGTCCTTCCCACAACCAAC PRIMER_LEFT_34=53,20 PRIMER_RIGHT_34=291,19 PRIMER_LEFT_34_TM=60.134 PRIMER_RIGHT_34_TM=60.207 PRIMER_LEFT_34_GC_PERCENT=50.000 PRIMER_RIGHT_34_GC_PERCENT=57.895 PRIMER_LEFT_34_SELF_ANY=4.00 PRIMER_RIGHT_34_SELF_ANY=3.00 PRIMER_LEFT_34_SELF_END=4.00 PRIMER_RIGHT_34_SELF_END=0.00 PRIMER_LEFT_34_END_STABILITY=11.2000 PRIMER_RIGHT_34_END_STABILITY=8.2000 PRIMER_PAIR_34_COMPL_ANY=5.00 PRIMER_PAIR_34_COMPL_END=2.00 PRIMER_PAIR_34_PRODUCT_SIZE=239 PRIMER_PAIR_35_PENALTY=1.340600 PRIMER_LEFT_35_PENALTY=0.133741 PRIMER_RIGHT_35_PENALTY=1.206859 PRIMER_LEFT_35_SEQUENCE=CAGCTGAGACTTTGAAGGCC PRIMER_RIGHT_35_SEQUENCE=GTCCTTCCCACAACCAACC PRIMER_LEFT_35=52,20 PRIMER_RIGHT_35=290,19 PRIMER_LEFT_35_TM=60.134 PRIMER_RIGHT_35_TM=60.207 PRIMER_LEFT_35_GC_PERCENT=55.000 PRIMER_RIGHT_35_GC_PERCENT=57.895 PRIMER_LEFT_35_SELF_ANY=6.00 PRIMER_RIGHT_35_SELF_ANY=2.00 PRIMER_LEFT_35_SELF_END=4.00 PRIMER_RIGHT_35_SELF_END=0.00 PRIMER_LEFT_35_END_STABILITY=10.9000 PRIMER_RIGHT_35_END_STABILITY=8.2000 PRIMER_PAIR_35_COMPL_ANY=5.00 PRIMER_PAIR_35_COMPL_END=1.00 PRIMER_PAIR_35_PRODUCT_SIZE=239 PRIMER_PAIR_36_PENALTY=1.340600 PRIMER_LEFT_36_PENALTY=0.133741 PRIMER_RIGHT_36_PENALTY=1.206859 PRIMER_LEFT_36_SEQUENCE=CAGCTGAGACTTTGAAGGCC PRIMER_RIGHT_36_SEQUENCE=GGTCCTTCCCACAACCAAC PRIMER_LEFT_36=52,20 PRIMER_RIGHT_36=291,19 PRIMER_LEFT_36_TM=60.134 PRIMER_RIGHT_36_TM=60.207 PRIMER_LEFT_36_GC_PERCENT=55.000 PRIMER_RIGHT_36_GC_PERCENT=57.895 PRIMER_LEFT_36_SELF_ANY=6.00 PRIMER_RIGHT_36_SELF_ANY=3.00 PRIMER_LEFT_36_SELF_END=4.00 PRIMER_RIGHT_36_SELF_END=0.00 PRIMER_LEFT_36_END_STABILITY=10.9000 PRIMER_RIGHT_36_END_STABILITY=8.2000 PRIMER_PAIR_36_COMPL_ANY=5.00 PRIMER_PAIR_36_COMPL_END=2.00 PRIMER_PAIR_36_PRODUCT_SIZE=240 PRIMER_PAIR_37_PENALTY=1.350422 PRIMER_LEFT_37_PENALTY=0.003322 PRIMER_RIGHT_37_PENALTY=1.347100 PRIMER_LEFT_37_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_37_SEQUENCE=TCCTTCCCACAACCAACCT PRIMER_LEFT_37=54,20 PRIMER_RIGHT_37=289,19 PRIMER_LEFT_37_TM=59.997 PRIMER_RIGHT_37_TM=60.347 PRIMER_LEFT_37_GC_PERCENT=55.000 PRIMER_RIGHT_37_GC_PERCENT=52.632 PRIMER_LEFT_37_SELF_ANY=4.00 PRIMER_RIGHT_37_SELF_ANY=2.00 PRIMER_LEFT_37_SELF_END=3.00 PRIMER_RIGHT_37_SELF_END=0.00 PRIMER_LEFT_37_END_STABILITY=9.4000 PRIMER_RIGHT_37_END_STABILITY=7.9000 PRIMER_PAIR_37_COMPL_ANY=5.00 PRIMER_PAIR_37_COMPL_END=0.00 PRIMER_PAIR_37_PRODUCT_SIZE=236 PRIMER_PAIR_38_PENALTY=1.350422 PRIMER_LEFT_38_PENALTY=0.003322 PRIMER_RIGHT_38_PENALTY=1.347100 PRIMER_LEFT_38_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_38_SEQUENCE=AGGTCCTTCCCACAACCAA PRIMER_LEFT_38=54,20 PRIMER_RIGHT_38=292,19 PRIMER_LEFT_38_TM=59.997 PRIMER_RIGHT_38_TM=60.347 PRIMER_LEFT_38_GC_PERCENT=55.000 PRIMER_RIGHT_38_GC_PERCENT=52.632 PRIMER_LEFT_38_SELF_ANY=4.00 PRIMER_RIGHT_38_SELF_ANY=5.00 PRIMER_LEFT_38_SELF_END=3.00 PRIMER_RIGHT_38_SELF_END=0.00 PRIMER_LEFT_38_END_STABILITY=9.4000 PRIMER_RIGHT_38_END_STABILITY=8.2000 PRIMER_PAIR_38_COMPL_ANY=5.00 PRIMER_PAIR_38_COMPL_END=0.00 PRIMER_PAIR_38_PRODUCT_SIZE=239 PRIMER_PAIR_39_PENALTY=1.350422 PRIMER_LEFT_39_PENALTY=0.003322 PRIMER_RIGHT_39_PENALTY=1.347100 PRIMER_LEFT_39_SEQUENCE=GCTGAGACTTTGAAGGCCAC PRIMER_RIGHT_39_SEQUENCE=AAGGTCCTTCCCACAACCA PRIMER_LEFT_39=54,20 PRIMER_RIGHT_39=293,19 PRIMER_LEFT_39_TM=59.997 PRIMER_RIGHT_39_TM=60.347 PRIMER_LEFT_39_GC_PERCENT=55.000 PRIMER_RIGHT_39_GC_PERCENT=52.632 PRIMER_LEFT_39_SELF_ANY=4.00 PRIMER_RIGHT_39_SELF_ANY=7.00 PRIMER_LEFT_39_SELF_END=3.00 PRIMER_RIGHT_39_SELF_END=0.00 PRIMER_LEFT_39_END_STABILITY=9.4000 PRIMER_RIGHT_39_END_STABILITY=8.2000 PRIMER_PAIR_39_COMPL_ANY=5.00 PRIMER_PAIR_39_COMPL_END=1.00 PRIMER_PAIR_39_PRODUCT_SIZE=240 = SEQUENCE_ID=06.RHAP34FE6.seq SEQUENCE_TEMPLATE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA SEQUENCE_INCLUDED_REGION=8,287 SEQUENCE_TARGET=61,68 PRIMER_LEFT_NUM_RETURNED=40 PRIMER_RIGHT_NUM_RETURNED=40 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=40 PRIMER_PAIR_0_PENALTY=2.096487 PRIMER_LEFT_0_PENALTY=1.153194 PRIMER_RIGHT_0_PENALTY=0.943292 PRIMER_LEFT_0_SEQUENCE=CAACCCAAAAATACAGACGGA PRIMER_RIGHT_0_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_0=9,21 PRIMER_RIGHT_0=148,20 PRIMER_LEFT_0_TM=59.847 PRIMER_RIGHT_0_TM=60.943 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=140 PRIMER_PAIR_1_PENALTY=2.623573 PRIMER_LEFT_1_PENALTY=1.153194 PRIMER_RIGHT_1_PENALTY=1.470379 PRIMER_LEFT_1_SEQUENCE=CAACCCAAAAATACAGACGGA PRIMER_RIGHT_1_SEQUENCE=CCCTGTGTTACCCGTCTCA PRIMER_LEFT_1=9,21 PRIMER_RIGHT_1=148,19 PRIMER_LEFT_1_TM=59.847 PRIMER_RIGHT_1_TM=59.530 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=140 PRIMER_PAIR_2_PENALTY=2.943089 PRIMER_LEFT_2_PENALTY=1.999797 PRIMER_RIGHT_2_PENALTY=0.943292 PRIMER_LEFT_2_SEQUENCE=CAACCCAAAAATACAGACGG PRIMER_RIGHT_2_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_2=9,20 PRIMER_RIGHT_2=148,20 PRIMER_LEFT_2_TM=58.000 PRIMER_RIGHT_2_TM=60.943 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.6000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=5.00 PRIMER_PAIR_2_PRODUCT_SIZE=140 PRIMER_PAIR_3_PENALTY=2.943089 PRIMER_LEFT_3_PENALTY=1.999797 PRIMER_RIGHT_3_PENALTY=0.943292 PRIMER_LEFT_3_SEQUENCE=CCAACCCAAAAATACAGACG PRIMER_RIGHT_3_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_3=8,20 PRIMER_RIGHT_3=148,20 PRIMER_LEFT_3_TM=58.000 PRIMER_RIGHT_3_TM=60.943 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=141 PRIMER_PAIR_4_PENALTY=3.406904 PRIMER_LEFT_4_PENALTY=2.463612 PRIMER_RIGHT_4_PENALTY=0.943292 PRIMER_LEFT_4_SEQUENCE=CCAACCCAAAAATACAGACGG PRIMER_RIGHT_4_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_4=8,21 PRIMER_RIGHT_4=148,20 PRIMER_LEFT_4_TM=61.464 PRIMER_RIGHT_4_TM=60.943 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.6000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=5.00 PRIMER_PAIR_4_PRODUCT_SIZE=141 PRIMER_PAIR_5_PENALTY=3.470176 PRIMER_LEFT_5_PENALTY=1.999797 PRIMER_RIGHT_5_PENALTY=1.470379 PRIMER_LEFT_5_SEQUENCE=CAACCCAAAAATACAGACGG PRIMER_RIGHT_5_SEQUENCE=CCCTGTGTTACCCGTCTCA PRIMER_LEFT_5=9,20 PRIMER_RIGHT_5=148,19 PRIMER_LEFT_5_TM=58.000 PRIMER_RIGHT_5_TM=59.530 PRIMER_LEFT_5_GC_PERCENT=45.000 PRIMER_RIGHT_5_GC_PERCENT=57.895 PRIMER_LEFT_5_SELF_ANY=2.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=2.00 PRIMER_LEFT_5_END_STABILITY=9.6000 PRIMER_RIGHT_5_END_STABILITY=6.7000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=4.00 PRIMER_PAIR_5_PRODUCT_SIZE=140 PRIMER_PAIR_6_PENALTY=3.470176 PRIMER_LEFT_6_PENALTY=1.999797 PRIMER_RIGHT_6_PENALTY=1.470379 PRIMER_LEFT_6_SEQUENCE=CCAACCCAAAAATACAGACG PRIMER_RIGHT_6_SEQUENCE=CCCTGTGTTACCCGTCTCA PRIMER_LEFT_6=8,20 PRIMER_RIGHT_6=148,19 PRIMER_LEFT_6_TM=58.000 PRIMER_RIGHT_6_TM=59.530 PRIMER_LEFT_6_GC_PERCENT=45.000 PRIMER_RIGHT_6_GC_PERCENT=57.895 PRIMER_LEFT_6_SELF_ANY=2.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_END_STABILITY=8.1000 PRIMER_RIGHT_6_END_STABILITY=6.7000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=141 PRIMER_PAIR_7_PENALTY=3.876831 PRIMER_LEFT_7_PENALTY=2.933539 PRIMER_RIGHT_7_PENALTY=0.943292 PRIMER_LEFT_7_SEQUENCE=AACCCAAAAATACAGACGGA PRIMER_RIGHT_7_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_7=10,20 PRIMER_RIGHT_7=148,20 PRIMER_LEFT_7_TM=57.066 PRIMER_RIGHT_7_TM=60.943 PRIMER_LEFT_7_GC_PERCENT=40.000 PRIMER_RIGHT_7_GC_PERCENT=55.000 PRIMER_LEFT_7_SELF_ANY=2.00 PRIMER_RIGHT_7_SELF_ANY=3.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=9.6000 PRIMER_RIGHT_7_END_STABILITY=7.0000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=4.00 PRIMER_PAIR_7_PRODUCT_SIZE=139 PRIMER_PAIR_8_PENALTY=3.933991 PRIMER_LEFT_8_PENALTY=2.463612 PRIMER_RIGHT_8_PENALTY=1.470379 PRIMER_LEFT_8_SEQUENCE=CCAACCCAAAAATACAGACGG PRIMER_RIGHT_8_SEQUENCE=CCCTGTGTTACCCGTCTCA PRIMER_LEFT_8=8,21 PRIMER_RIGHT_8=148,19 PRIMER_LEFT_8_TM=61.464 PRIMER_RIGHT_8_TM=59.530 PRIMER_LEFT_8_GC_PERCENT=47.619 PRIMER_RIGHT_8_GC_PERCENT=57.895 PRIMER_LEFT_8_SELF_ANY=2.00 PRIMER_RIGHT_8_SELF_ANY=3.00 PRIMER_LEFT_8_SELF_END=1.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_END_STABILITY=9.6000 PRIMER_RIGHT_8_END_STABILITY=6.7000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=4.00 PRIMER_PAIR_8_PRODUCT_SIZE=141 PRIMER_PAIR_9_PENALTY=4.403918 PRIMER_LEFT_9_PENALTY=2.933539 PRIMER_RIGHT_9_PENALTY=1.470379 PRIMER_LEFT_9_SEQUENCE=AACCCAAAAATACAGACGGA PRIMER_RIGHT_9_SEQUENCE=CCCTGTGTTACCCGTCTCA PRIMER_LEFT_9=10,20 PRIMER_RIGHT_9=148,19 PRIMER_LEFT_9_TM=57.066 PRIMER_RIGHT_9_TM=59.530 PRIMER_LEFT_9_GC_PERCENT=40.000 PRIMER_RIGHT_9_GC_PERCENT=57.895 PRIMER_LEFT_9_SELF_ANY=2.00 PRIMER_RIGHT_9_SELF_ANY=3.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_END_STABILITY=9.6000 PRIMER_RIGHT_9_END_STABILITY=6.7000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=3.00 PRIMER_PAIR_9_PRODUCT_SIZE=139 PRIMER_PAIR_10_PENALTY=4.451682 PRIMER_LEFT_10_PENALTY=4.436430 PRIMER_RIGHT_10_PENALTY=0.015253 PRIMER_LEFT_10_SEQUENCE=CACAAACAGAGACACAGACACAGA PRIMER_RIGHT_10_SEQUENCE=CTTCTGTGCTTTCCCGCTAC PRIMER_LEFT_10=36,24 PRIMER_RIGHT_10=210,20 PRIMER_LEFT_10_TM=60.436 PRIMER_RIGHT_10_TM=60.015 PRIMER_LEFT_10_GC_PERCENT=45.833 PRIMER_RIGHT_10_GC_PERCENT=55.000 PRIMER_LEFT_10_SELF_ANY=1.00 PRIMER_RIGHT_10_SELF_ANY=2.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_END_STABILITY=6.4000 PRIMER_RIGHT_10_END_STABILITY=6.9000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=4.00 PRIMER_PAIR_10_PRODUCT_SIZE=175 PRIMER_PAIR_11_PENALTY=4.497659 PRIMER_LEFT_11_PENALTY=4.436430 PRIMER_RIGHT_11_PENALTY=0.061230 PRIMER_LEFT_11_SEQUENCE=CACAAACAGAGACACAGACACAGA PRIMER_RIGHT_11_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_11=36,24 PRIMER_RIGHT_11=203,20 PRIMER_LEFT_11_TM=60.436 PRIMER_RIGHT_11_TM=60.061 PRIMER_LEFT_11_GC_PERCENT=45.833 PRIMER_RIGHT_11_GC_PERCENT=55.000 PRIMER_LEFT_11_SELF_ANY=1.00 PRIMER_RIGHT_11_SELF_ANY=2.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_END_STABILITY=6.4000 PRIMER_RIGHT_11_END_STABILITY=5.9000 PRIMER_PAIR_11_COMPL_ANY=3.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PAIR_11_PRODUCT_SIZE=168 PRIMER_PAIR_12_PENALTY=4.529170 PRIMER_LEFT_12_PENALTY=4.513917 PRIMER_RIGHT_12_PENALTY=0.015253 PRIMER_LEFT_12_SEQUENCE=CACAAACAGAGACACAGACACAG PRIMER_RIGHT_12_SEQUENCE=CTTCTGTGCTTTCCCGCTAC PRIMER_LEFT_12=36,23 PRIMER_RIGHT_12=210,20 PRIMER_LEFT_12_TM=58.486 PRIMER_RIGHT_12_TM=60.015 PRIMER_LEFT_12_GC_PERCENT=47.826 PRIMER_RIGHT_12_GC_PERCENT=55.000 PRIMER_LEFT_12_SELF_ANY=1.00 PRIMER_RIGHT_12_SELF_ANY=2.00 PRIMER_LEFT_12_SELF_END=1.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_END_STABILITY=6.7000 PRIMER_RIGHT_12_END_STABILITY=6.9000 PRIMER_PAIR_12_COMPL_ANY=6.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PAIR_12_PRODUCT_SIZE=175 PRIMER_PAIR_13_PENALTY=4.575147 PRIMER_LEFT_13_PENALTY=4.513917 PRIMER_RIGHT_13_PENALTY=0.061230 PRIMER_LEFT_13_SEQUENCE=CACAAACAGAGACACAGACACAG PRIMER_RIGHT_13_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_13=36,23 PRIMER_RIGHT_13=203,20 PRIMER_LEFT_13_TM=58.486 PRIMER_RIGHT_13_TM=60.061 PRIMER_LEFT_13_GC_PERCENT=47.826 PRIMER_RIGHT_13_GC_PERCENT=55.000 PRIMER_LEFT_13_SELF_ANY=1.00 PRIMER_RIGHT_13_SELF_ANY=2.00 PRIMER_LEFT_13_SELF_END=1.00 PRIMER_RIGHT_13_SELF_END=0.00 PRIMER_LEFT_13_END_STABILITY=6.7000 PRIMER_RIGHT_13_END_STABILITY=5.9000 PRIMER_PAIR_13_COMPL_ANY=3.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PAIR_13_PRODUCT_SIZE=168 PRIMER_PAIR_14_PENALTY=4.670466 PRIMER_LEFT_14_PENALTY=4.609236 PRIMER_RIGHT_14_PENALTY=0.061230 PRIMER_LEFT_14_SEQUENCE=ACACAAACAGAGACACAGACACAG PRIMER_RIGHT_14_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_14=35,24 PRIMER_RIGHT_14=203,20 PRIMER_LEFT_14_TM=59.391 PRIMER_RIGHT_14_TM=60.061 PRIMER_LEFT_14_GC_PERCENT=45.833 PRIMER_RIGHT_14_GC_PERCENT=55.000 PRIMER_LEFT_14_SELF_ANY=1.00 PRIMER_RIGHT_14_SELF_ANY=2.00 PRIMER_LEFT_14_SELF_END=1.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=5.9000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PAIR_14_PRODUCT_SIZE=169 PRIMER_PAIR_15_PENALTY=4.684266 PRIMER_LEFT_15_PENALTY=4.669014 PRIMER_RIGHT_15_PENALTY=0.015253 PRIMER_LEFT_15_SEQUENCE=CACAAACAGAGACACAGACACA PRIMER_RIGHT_15_SEQUENCE=CTTCTGTGCTTTCCCGCTAC PRIMER_LEFT_15=36,22 PRIMER_RIGHT_15=210,20 PRIMER_LEFT_15_TM=57.331 PRIMER_RIGHT_15_TM=60.015 PRIMER_LEFT_15_GC_PERCENT=45.455 PRIMER_RIGHT_15_GC_PERCENT=55.000 PRIMER_LEFT_15_SELF_ANY=1.00 PRIMER_RIGHT_15_SELF_ANY=2.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_END_STABILITY=6.4000 PRIMER_RIGHT_15_END_STABILITY=6.9000 PRIMER_PAIR_15_COMPL_ANY=6.00 PRIMER_PAIR_15_COMPL_END=2.00 PRIMER_PAIR_15_PRODUCT_SIZE=175 PRIMER_PAIR_16_PENALTY=4.730243 PRIMER_LEFT_16_PENALTY=4.669014 PRIMER_RIGHT_16_PENALTY=0.061230 PRIMER_LEFT_16_SEQUENCE=CACAAACAGAGACACAGACACA PRIMER_RIGHT_16_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_16=36,22 PRIMER_RIGHT_16=203,20 PRIMER_LEFT_16_TM=57.331 PRIMER_RIGHT_16_TM=60.061 PRIMER_LEFT_16_GC_PERCENT=45.455 PRIMER_RIGHT_16_GC_PERCENT=55.000 PRIMER_LEFT_16_SELF_ANY=1.00 PRIMER_RIGHT_16_SELF_ANY=2.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_END_STABILITY=6.4000 PRIMER_RIGHT_16_END_STABILITY=5.9000 PRIMER_PAIR_16_COMPL_ANY=3.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PAIR_16_PRODUCT_SIZE=168 PRIMER_PAIR_17_PENALTY=4.730243 PRIMER_LEFT_17_PENALTY=4.669014 PRIMER_RIGHT_17_PENALTY=0.061230 PRIMER_LEFT_17_SEQUENCE=CACACAAACAGAGACACAGACA PRIMER_RIGHT_17_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_17=34,22 PRIMER_RIGHT_17=203,20 PRIMER_LEFT_17_TM=57.331 PRIMER_RIGHT_17_TM=60.061 PRIMER_LEFT_17_GC_PERCENT=45.455 PRIMER_RIGHT_17_GC_PERCENT=55.000 PRIMER_LEFT_17_SELF_ANY=1.00 PRIMER_RIGHT_17_SELF_ANY=2.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_END_STABILITY=6.4000 PRIMER_RIGHT_17_END_STABILITY=5.9000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PAIR_17_PRODUCT_SIZE=170 PRIMER_PAIR_18_PENALTY=4.730243 PRIMER_LEFT_18_PENALTY=4.669014 PRIMER_RIGHT_18_PENALTY=0.061230 PRIMER_LEFT_18_SEQUENCE=CACACACAAACAGAGACACAGA PRIMER_RIGHT_18_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_18=32,22 PRIMER_RIGHT_18=203,20 PRIMER_LEFT_18_TM=57.331 PRIMER_RIGHT_18_TM=60.061 PRIMER_LEFT_18_GC_PERCENT=45.455 PRIMER_RIGHT_18_GC_PERCENT=55.000 PRIMER_LEFT_18_SELF_ANY=1.00 PRIMER_RIGHT_18_SELF_ANY=2.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=0.00 PRIMER_LEFT_18_END_STABILITY=6.4000 PRIMER_RIGHT_18_END_STABILITY=5.9000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PAIR_18_PRODUCT_SIZE=172 PRIMER_PAIR_19_PENALTY=4.730755 PRIMER_LEFT_19_PENALTY=4.669525 PRIMER_RIGHT_19_PENALTY=0.061230 PRIMER_LEFT_19_SEQUENCE=ACACAAACAGAGACACAGACACA PRIMER_RIGHT_19_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_19=35,23 PRIMER_RIGHT_19=203,20 PRIMER_LEFT_19_TM=58.330 PRIMER_RIGHT_19_TM=60.061 PRIMER_LEFT_19_GC_PERCENT=43.478 PRIMER_RIGHT_19_GC_PERCENT=55.000 PRIMER_LEFT_19_SELF_ANY=1.00 PRIMER_RIGHT_19_SELF_ANY=2.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_END_STABILITY=6.4000 PRIMER_RIGHT_19_END_STABILITY=5.9000 PRIMER_PAIR_19_COMPL_ANY=3.00 PRIMER_PAIR_19_COMPL_END=1.00 PRIMER_PAIR_19_PRODUCT_SIZE=169 PRIMER_PAIR_20_PENALTY=4.730755 PRIMER_LEFT_20_PENALTY=4.669525 PRIMER_RIGHT_20_PENALTY=0.061230 PRIMER_LEFT_20_SEQUENCE=CACACAAACAGAGACACAGACAC PRIMER_RIGHT_20_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_20=34,23 PRIMER_RIGHT_20=203,20 PRIMER_LEFT_20_TM=58.330 PRIMER_RIGHT_20_TM=60.061 PRIMER_LEFT_20_GC_PERCENT=47.826 PRIMER_RIGHT_20_GC_PERCENT=55.000 PRIMER_LEFT_20_SELF_ANY=1.00 PRIMER_RIGHT_20_SELF_ANY=2.00 PRIMER_LEFT_20_SELF_END=0.00 PRIMER_RIGHT_20_SELF_END=0.00 PRIMER_LEFT_20_END_STABILITY=6.1000 PRIMER_RIGHT_20_END_STABILITY=5.9000 PRIMER_PAIR_20_COMPL_ANY=3.00 PRIMER_PAIR_20_COMPL_END=0.00 PRIMER_PAIR_20_PRODUCT_SIZE=170 PRIMER_PAIR_21_PENALTY=4.730755 PRIMER_LEFT_21_PENALTY=4.669525 PRIMER_RIGHT_21_PENALTY=0.061230 PRIMER_LEFT_21_SEQUENCE=ACACACAAACAGAGACACAGACA PRIMER_RIGHT_21_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_21=33,23 PRIMER_RIGHT_21=203,20 PRIMER_LEFT_21_TM=58.330 PRIMER_RIGHT_21_TM=60.061 PRIMER_LEFT_21_GC_PERCENT=43.478 PRIMER_RIGHT_21_GC_PERCENT=55.000 PRIMER_LEFT_21_SELF_ANY=1.00 PRIMER_RIGHT_21_SELF_ANY=2.00 PRIMER_LEFT_21_SELF_END=0.00 PRIMER_RIGHT_21_SELF_END=0.00 PRIMER_LEFT_21_END_STABILITY=6.4000 PRIMER_RIGHT_21_END_STABILITY=5.9000 PRIMER_PAIR_21_COMPL_ANY=3.00 PRIMER_PAIR_21_COMPL_END=1.00 PRIMER_PAIR_21_PRODUCT_SIZE=171 PRIMER_PAIR_22_PENALTY=4.730755 PRIMER_LEFT_22_PENALTY=4.669525 PRIMER_RIGHT_22_PENALTY=0.061230 PRIMER_LEFT_22_SEQUENCE=CACACACAAACAGAGACACAGAC PRIMER_RIGHT_22_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_22=32,23 PRIMER_RIGHT_22=203,20 PRIMER_LEFT_22_TM=58.330 PRIMER_RIGHT_22_TM=60.061 PRIMER_LEFT_22_GC_PERCENT=47.826 PRIMER_RIGHT_22_GC_PERCENT=55.000 PRIMER_LEFT_22_SELF_ANY=1.00 PRIMER_RIGHT_22_SELF_ANY=2.00 PRIMER_LEFT_22_SELF_END=1.00 PRIMER_RIGHT_22_SELF_END=0.00 PRIMER_LEFT_22_END_STABILITY=6.4000 PRIMER_RIGHT_22_END_STABILITY=5.9000 PRIMER_PAIR_22_COMPL_ANY=3.00 PRIMER_PAIR_22_COMPL_END=1.00 PRIMER_PAIR_22_PRODUCT_SIZE=172 PRIMER_PAIR_23_PENALTY=4.730755 PRIMER_LEFT_23_PENALTY=4.669525 PRIMER_RIGHT_23_PENALTY=0.061230 PRIMER_LEFT_23_SEQUENCE=ACACACACAAACAGAGACACAGA PRIMER_RIGHT_23_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_23=31,23 PRIMER_RIGHT_23=203,20 PRIMER_LEFT_23_TM=58.330 PRIMER_RIGHT_23_TM=60.061 PRIMER_LEFT_23_GC_PERCENT=43.478 PRIMER_RIGHT_23_GC_PERCENT=55.000 PRIMER_LEFT_23_SELF_ANY=1.00 PRIMER_RIGHT_23_SELF_ANY=2.00 PRIMER_LEFT_23_SELF_END=0.00 PRIMER_RIGHT_23_SELF_END=0.00 PRIMER_LEFT_23_END_STABILITY=6.4000 PRIMER_RIGHT_23_END_STABILITY=5.9000 PRIMER_PAIR_23_COMPL_ANY=3.00 PRIMER_PAIR_23_COMPL_END=2.00 PRIMER_PAIR_23_PRODUCT_SIZE=173 PRIMER_PAIR_24_PENALTY=4.812339 PRIMER_LEFT_24_PENALTY=4.751110 PRIMER_RIGHT_24_PENALTY=0.061230 PRIMER_LEFT_24_SEQUENCE=ACACACAAACAGAGACACAGACAC PRIMER_RIGHT_24_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_24=33,24 PRIMER_RIGHT_24=203,20 PRIMER_LEFT_24_TM=59.249 PRIMER_RIGHT_24_TM=60.061 PRIMER_LEFT_24_GC_PERCENT=45.833 PRIMER_RIGHT_24_GC_PERCENT=55.000 PRIMER_LEFT_24_SELF_ANY=1.00 PRIMER_RIGHT_24_SELF_ANY=2.00 PRIMER_LEFT_24_SELF_END=0.00 PRIMER_RIGHT_24_SELF_END=0.00 PRIMER_LEFT_24_END_STABILITY=6.1000 PRIMER_RIGHT_24_END_STABILITY=5.9000 PRIMER_PAIR_24_COMPL_ANY=3.00 PRIMER_PAIR_24_COMPL_END=0.00 PRIMER_PAIR_24_PRODUCT_SIZE=171 PRIMER_PAIR_25_PENALTY=4.812339 PRIMER_LEFT_25_PENALTY=4.751110 PRIMER_RIGHT_25_PENALTY=0.061230 PRIMER_LEFT_25_SEQUENCE=ACACACACAAACAGAGACACAGAC PRIMER_RIGHT_25_SEQUENCE=GCTTTCCCGCTACCCTATTC PRIMER_LEFT_25=31,24 PRIMER_RIGHT_25=203,20 PRIMER_LEFT_25_TM=59.249 PRIMER_RIGHT_25_TM=60.061 PRIMER_LEFT_25_GC_PERCENT=45.833 PRIMER_RIGHT_25_GC_PERCENT=55.000 PRIMER_LEFT_25_SELF_ANY=1.00 PRIMER_RIGHT_25_SELF_ANY=2.00 PRIMER_LEFT_25_SELF_END=1.00 PRIMER_RIGHT_25_SELF_END=0.00 PRIMER_LEFT_25_END_STABILITY=6.4000 PRIMER_RIGHT_25_END_STABILITY=5.9000 PRIMER_PAIR_25_COMPL_ANY=3.00 PRIMER_PAIR_25_COMPL_END=1.00 PRIMER_PAIR_25_PRODUCT_SIZE=173 PRIMER_PAIR_26_PENALTY=4.907661 PRIMER_LEFT_26_PENALTY=4.436430 PRIMER_RIGHT_26_PENALTY=0.471231 PRIMER_LEFT_26_SEQUENCE=CACAAACAGAGACACAGACACAGA PRIMER_RIGHT_26_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_26=36,24 PRIMER_RIGHT_26=205,20 PRIMER_LEFT_26_TM=60.436 PRIMER_RIGHT_26_TM=60.471 PRIMER_LEFT_26_GC_PERCENT=45.833 PRIMER_RIGHT_26_GC_PERCENT=55.000 PRIMER_LEFT_26_SELF_ANY=1.00 PRIMER_RIGHT_26_SELF_ANY=2.00 PRIMER_LEFT_26_SELF_END=0.00 PRIMER_RIGHT_26_SELF_END=2.00 PRIMER_LEFT_26_END_STABILITY=6.4000 PRIMER_RIGHT_26_END_STABILITY=7.1000 PRIMER_PAIR_26_COMPL_ANY=3.00 PRIMER_PAIR_26_COMPL_END=1.00 PRIMER_PAIR_26_PRODUCT_SIZE=170 PRIMER_PAIR_27_PENALTY=4.985149 PRIMER_LEFT_27_PENALTY=4.513917 PRIMER_RIGHT_27_PENALTY=0.471231 PRIMER_LEFT_27_SEQUENCE=CACAAACAGAGACACAGACACAG PRIMER_RIGHT_27_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_27=36,23 PRIMER_RIGHT_27=205,20 PRIMER_LEFT_27_TM=58.486 PRIMER_RIGHT_27_TM=60.471 PRIMER_LEFT_27_GC_PERCENT=47.826 PRIMER_RIGHT_27_GC_PERCENT=55.000 PRIMER_LEFT_27_SELF_ANY=1.00 PRIMER_RIGHT_27_SELF_ANY=2.00 PRIMER_LEFT_27_SELF_END=1.00 PRIMER_RIGHT_27_SELF_END=2.00 PRIMER_LEFT_27_END_STABILITY=6.7000 PRIMER_RIGHT_27_END_STABILITY=7.1000 PRIMER_PAIR_27_COMPL_ANY=3.00 PRIMER_PAIR_27_COMPL_END=2.00 PRIMER_PAIR_27_PRODUCT_SIZE=170 PRIMER_PAIR_28_PENALTY=5.080467 PRIMER_LEFT_28_PENALTY=4.609236 PRIMER_RIGHT_28_PENALTY=0.471231 PRIMER_LEFT_28_SEQUENCE=ACACAAACAGAGACACAGACACAG PRIMER_RIGHT_28_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_28=35,24 PRIMER_RIGHT_28=205,20 PRIMER_LEFT_28_TM=59.391 PRIMER_RIGHT_28_TM=60.471 PRIMER_LEFT_28_GC_PERCENT=45.833 PRIMER_RIGHT_28_GC_PERCENT=55.000 PRIMER_LEFT_28_SELF_ANY=1.00 PRIMER_RIGHT_28_SELF_ANY=2.00 PRIMER_LEFT_28_SELF_END=1.00 PRIMER_RIGHT_28_SELF_END=2.00 PRIMER_LEFT_28_END_STABILITY=6.7000 PRIMER_RIGHT_28_END_STABILITY=7.1000 PRIMER_PAIR_28_COMPL_ANY=3.00 PRIMER_PAIR_28_COMPL_END=2.00 PRIMER_PAIR_28_PRODUCT_SIZE=171 PRIMER_PAIR_29_PENALTY=5.095753 PRIMER_LEFT_29_PENALTY=1.153194 PRIMER_RIGHT_29_PENALTY=3.942558 PRIMER_LEFT_29_SEQUENCE=CAACCCAAAAATACAGACGGA PRIMER_RIGHT_29_SEQUENCE=CCTGTGTTACCCGTCTCAA PRIMER_LEFT_29=9,21 PRIMER_RIGHT_29=147,19 PRIMER_LEFT_29_TM=59.847 PRIMER_RIGHT_29_TM=57.057 PRIMER_LEFT_29_GC_PERCENT=42.857 PRIMER_RIGHT_29_GC_PERCENT=52.632 PRIMER_LEFT_29_SELF_ANY=2.00 PRIMER_RIGHT_29_SELF_ANY=3.00 PRIMER_LEFT_29_SELF_END=0.00 PRIMER_RIGHT_29_SELF_END=0.00 PRIMER_LEFT_29_END_STABILITY=9.6000 PRIMER_RIGHT_29_END_STABILITY=7.0000 PRIMER_PAIR_29_COMPL_ANY=6.00 PRIMER_PAIR_29_COMPL_END=4.00 PRIMER_PAIR_29_PRODUCT_SIZE=139 PRIMER_PAIR_30_PENALTY=5.140245 PRIMER_LEFT_30_PENALTY=4.669014 PRIMER_RIGHT_30_PENALTY=0.471231 PRIMER_LEFT_30_SEQUENCE=CACAAACAGAGACACAGACACA PRIMER_RIGHT_30_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_30=36,22 PRIMER_RIGHT_30=205,20 PRIMER_LEFT_30_TM=57.331 PRIMER_RIGHT_30_TM=60.471 PRIMER_LEFT_30_GC_PERCENT=45.455 PRIMER_RIGHT_30_GC_PERCENT=55.000 PRIMER_LEFT_30_SELF_ANY=1.00 PRIMER_RIGHT_30_SELF_ANY=2.00 PRIMER_LEFT_30_SELF_END=0.00 PRIMER_RIGHT_30_SELF_END=2.00 PRIMER_LEFT_30_END_STABILITY=6.4000 PRIMER_RIGHT_30_END_STABILITY=7.1000 PRIMER_PAIR_30_COMPL_ANY=3.00 PRIMER_PAIR_30_COMPL_END=1.00 PRIMER_PAIR_30_PRODUCT_SIZE=170 PRIMER_PAIR_31_PENALTY=5.140245 PRIMER_LEFT_31_PENALTY=4.669014 PRIMER_RIGHT_31_PENALTY=0.471231 PRIMER_LEFT_31_SEQUENCE=CACACAAACAGAGACACAGACA PRIMER_RIGHT_31_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_31=34,22 PRIMER_RIGHT_31=205,20 PRIMER_LEFT_31_TM=57.331 PRIMER_RIGHT_31_TM=60.471 PRIMER_LEFT_31_GC_PERCENT=45.455 PRIMER_RIGHT_31_GC_PERCENT=55.000 PRIMER_LEFT_31_SELF_ANY=1.00 PRIMER_RIGHT_31_SELF_ANY=2.00 PRIMER_LEFT_31_SELF_END=0.00 PRIMER_RIGHT_31_SELF_END=2.00 PRIMER_LEFT_31_END_STABILITY=6.4000 PRIMER_RIGHT_31_END_STABILITY=7.1000 PRIMER_PAIR_31_COMPL_ANY=3.00 PRIMER_PAIR_31_COMPL_END=1.00 PRIMER_PAIR_31_PRODUCT_SIZE=172 PRIMER_PAIR_32_PENALTY=5.140245 PRIMER_LEFT_32_PENALTY=4.669014 PRIMER_RIGHT_32_PENALTY=0.471231 PRIMER_LEFT_32_SEQUENCE=CACACACAAACAGAGACACAGA PRIMER_RIGHT_32_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_32=32,22 PRIMER_RIGHT_32=205,20 PRIMER_LEFT_32_TM=57.331 PRIMER_RIGHT_32_TM=60.471 PRIMER_LEFT_32_GC_PERCENT=45.455 PRIMER_RIGHT_32_GC_PERCENT=55.000 PRIMER_LEFT_32_SELF_ANY=1.00 PRIMER_RIGHT_32_SELF_ANY=2.00 PRIMER_LEFT_32_SELF_END=0.00 PRIMER_RIGHT_32_SELF_END=2.00 PRIMER_LEFT_32_END_STABILITY=6.4000 PRIMER_RIGHT_32_END_STABILITY=7.1000 PRIMER_PAIR_32_COMPL_ANY=3.00 PRIMER_PAIR_32_COMPL_END=1.00 PRIMER_PAIR_32_PRODUCT_SIZE=174 PRIMER_PAIR_33_PENALTY=5.140757 PRIMER_LEFT_33_PENALTY=4.669525 PRIMER_RIGHT_33_PENALTY=0.471231 PRIMER_LEFT_33_SEQUENCE=ACACAAACAGAGACACAGACACA PRIMER_RIGHT_33_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_33=35,23 PRIMER_RIGHT_33=205,20 PRIMER_LEFT_33_TM=58.330 PRIMER_RIGHT_33_TM=60.471 PRIMER_LEFT_33_GC_PERCENT=43.478 PRIMER_RIGHT_33_GC_PERCENT=55.000 PRIMER_LEFT_33_SELF_ANY=1.00 PRIMER_RIGHT_33_SELF_ANY=2.00 PRIMER_LEFT_33_SELF_END=0.00 PRIMER_RIGHT_33_SELF_END=2.00 PRIMER_LEFT_33_END_STABILITY=6.4000 PRIMER_RIGHT_33_END_STABILITY=7.1000 PRIMER_PAIR_33_COMPL_ANY=3.00 PRIMER_PAIR_33_COMPL_END=1.00 PRIMER_PAIR_33_PRODUCT_SIZE=171 PRIMER_PAIR_34_PENALTY=5.140757 PRIMER_LEFT_34_PENALTY=4.669525 PRIMER_RIGHT_34_PENALTY=0.471231 PRIMER_LEFT_34_SEQUENCE=CACACAAACAGAGACACAGACAC PRIMER_RIGHT_34_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_34=34,23 PRIMER_RIGHT_34=205,20 PRIMER_LEFT_34_TM=58.330 PRIMER_RIGHT_34_TM=60.471 PRIMER_LEFT_34_GC_PERCENT=47.826 PRIMER_RIGHT_34_GC_PERCENT=55.000 PRIMER_LEFT_34_SELF_ANY=1.00 PRIMER_RIGHT_34_SELF_ANY=2.00 PRIMER_LEFT_34_SELF_END=0.00 PRIMER_RIGHT_34_SELF_END=2.00 PRIMER_LEFT_34_END_STABILITY=6.1000 PRIMER_RIGHT_34_END_STABILITY=7.1000 PRIMER_PAIR_34_COMPL_ANY=3.00 PRIMER_PAIR_34_COMPL_END=0.00 PRIMER_PAIR_34_PRODUCT_SIZE=172 PRIMER_PAIR_35_PENALTY=5.140757 PRIMER_LEFT_35_PENALTY=4.669525 PRIMER_RIGHT_35_PENALTY=0.471231 PRIMER_LEFT_35_SEQUENCE=ACACACAAACAGAGACACAGACA PRIMER_RIGHT_35_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_35=33,23 PRIMER_RIGHT_35=205,20 PRIMER_LEFT_35_TM=58.330 PRIMER_RIGHT_35_TM=60.471 PRIMER_LEFT_35_GC_PERCENT=43.478 PRIMER_RIGHT_35_GC_PERCENT=55.000 PRIMER_LEFT_35_SELF_ANY=1.00 PRIMER_RIGHT_35_SELF_ANY=2.00 PRIMER_LEFT_35_SELF_END=0.00 PRIMER_RIGHT_35_SELF_END=2.00 PRIMER_LEFT_35_END_STABILITY=6.4000 PRIMER_RIGHT_35_END_STABILITY=7.1000 PRIMER_PAIR_35_COMPL_ANY=3.00 PRIMER_PAIR_35_COMPL_END=1.00 PRIMER_PAIR_35_PRODUCT_SIZE=173 PRIMER_PAIR_36_PENALTY=5.140757 PRIMER_LEFT_36_PENALTY=4.669525 PRIMER_RIGHT_36_PENALTY=0.471231 PRIMER_LEFT_36_SEQUENCE=CACACACAAACAGAGACACAGAC PRIMER_RIGHT_36_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_36=32,23 PRIMER_RIGHT_36=205,20 PRIMER_LEFT_36_TM=58.330 PRIMER_RIGHT_36_TM=60.471 PRIMER_LEFT_36_GC_PERCENT=47.826 PRIMER_RIGHT_36_GC_PERCENT=55.000 PRIMER_LEFT_36_SELF_ANY=1.00 PRIMER_RIGHT_36_SELF_ANY=2.00 PRIMER_LEFT_36_SELF_END=1.00 PRIMER_RIGHT_36_SELF_END=2.00 PRIMER_LEFT_36_END_STABILITY=6.4000 PRIMER_RIGHT_36_END_STABILITY=7.1000 PRIMER_PAIR_36_COMPL_ANY=3.00 PRIMER_PAIR_36_COMPL_END=0.00 PRIMER_PAIR_36_PRODUCT_SIZE=174 PRIMER_PAIR_37_PENALTY=5.140757 PRIMER_LEFT_37_PENALTY=4.669525 PRIMER_RIGHT_37_PENALTY=0.471231 PRIMER_LEFT_37_SEQUENCE=ACACACACAAACAGAGACACAGA PRIMER_RIGHT_37_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_37=31,23 PRIMER_RIGHT_37=205,20 PRIMER_LEFT_37_TM=58.330 PRIMER_RIGHT_37_TM=60.471 PRIMER_LEFT_37_GC_PERCENT=43.478 PRIMER_RIGHT_37_GC_PERCENT=55.000 PRIMER_LEFT_37_SELF_ANY=1.00 PRIMER_RIGHT_37_SELF_ANY=2.00 PRIMER_LEFT_37_SELF_END=0.00 PRIMER_RIGHT_37_SELF_END=2.00 PRIMER_LEFT_37_END_STABILITY=6.4000 PRIMER_RIGHT_37_END_STABILITY=7.1000 PRIMER_PAIR_37_COMPL_ANY=3.00 PRIMER_PAIR_37_COMPL_END=1.00 PRIMER_PAIR_37_PRODUCT_SIZE=175 PRIMER_PAIR_38_PENALTY=5.222341 PRIMER_LEFT_38_PENALTY=4.751110 PRIMER_RIGHT_38_PENALTY=0.471231 PRIMER_LEFT_38_SEQUENCE=ACACACAAACAGAGACACAGACAC PRIMER_RIGHT_38_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_38=33,24 PRIMER_RIGHT_38=205,20 PRIMER_LEFT_38_TM=59.249 PRIMER_RIGHT_38_TM=60.471 PRIMER_LEFT_38_GC_PERCENT=45.833 PRIMER_RIGHT_38_GC_PERCENT=55.000 PRIMER_LEFT_38_SELF_ANY=1.00 PRIMER_RIGHT_38_SELF_ANY=2.00 PRIMER_LEFT_38_SELF_END=0.00 PRIMER_RIGHT_38_SELF_END=2.00 PRIMER_LEFT_38_END_STABILITY=6.1000 PRIMER_RIGHT_38_END_STABILITY=7.1000 PRIMER_PAIR_38_COMPL_ANY=3.00 PRIMER_PAIR_38_COMPL_END=0.00 PRIMER_PAIR_38_PRODUCT_SIZE=173 PRIMER_PAIR_39_PENALTY=5.222341 PRIMER_LEFT_39_PENALTY=4.751110 PRIMER_RIGHT_39_PENALTY=0.471231 PRIMER_LEFT_39_SEQUENCE=ACACACACAAACAGAGACACAGAC PRIMER_RIGHT_39_SEQUENCE=GTGCTTTCCCGCTACCCTAT PRIMER_LEFT_39=31,24 PRIMER_RIGHT_39=205,20 PRIMER_LEFT_39_TM=59.249 PRIMER_RIGHT_39_TM=60.471 PRIMER_LEFT_39_GC_PERCENT=45.833 PRIMER_RIGHT_39_GC_PERCENT=55.000 PRIMER_LEFT_39_SELF_ANY=1.00 PRIMER_RIGHT_39_SELF_ANY=2.00 PRIMER_LEFT_39_SELF_END=1.00 PRIMER_RIGHT_39_SELF_END=2.00 PRIMER_LEFT_39_END_STABILITY=6.4000 PRIMER_RIGHT_39_END_STABILITY=7.1000 PRIMER_PAIR_39_COMPL_ANY=3.00 PRIMER_PAIR_39_COMPL_END=0.00 PRIMER_PAIR_39_PRODUCT_SIZE=175 = SEQUENCE_ID=07.RHAP34FE7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT SEQUENCE_INCLUDED_REGION=18,283 SEQUENCE_TARGET=115,29 SEQUENCE_TARGET=145,20 SEQUENCE_TARGET=166,24 PRIMER_LEFT_NUM_RETURNED=40 PRIMER_RIGHT_NUM_RETURNED=40 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=40 PRIMER_PAIR_0_PENALTY=0.484594 PRIMER_LEFT_0_PENALTY=0.151542 PRIMER_RIGHT_0_PENALTY=0.333053 PRIMER_LEFT_0_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_0_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_0=76,20 PRIMER_RIGHT_0=238,20 PRIMER_LEFT_0_TM=60.152 PRIMER_RIGHT_0_TM=60.333 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=163 PRIMER_PAIR_1_PENALTY=0.681190 PRIMER_LEFT_1_PENALTY=0.151542 PRIMER_RIGHT_1_PENALTY=0.529649 PRIMER_LEFT_1_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_1_SEQUENCE=GCACGCATGTAGAGGTTCAG PRIMER_LEFT_1=76,20 PRIMER_RIGHT_1=235,20 PRIMER_LEFT_1_TM=60.152 PRIMER_RIGHT_1_TM=59.470 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=160 PRIMER_PAIR_2_PENALTY=0.789733 PRIMER_LEFT_2_PENALTY=0.151542 PRIMER_RIGHT_2_PENALTY=0.638191 PRIMER_LEFT_2_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_2_SEQUENCE=AGAGGTTCAGGTGGAGAGGG PRIMER_LEFT_2=76,20 PRIMER_RIGHT_2=225,20 PRIMER_LEFT_2_TM=60.152 PRIMER_RIGHT_2_TM=60.638 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=150 PRIMER_PAIR_3_PENALTY=0.828489 PRIMER_LEFT_3_PENALTY=0.151542 PRIMER_RIGHT_3_PENALTY=0.676947 PRIMER_LEFT_3_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_3_SEQUENCE=GTGCACGCATGTAGAGGTTC PRIMER_LEFT_3=76,20 PRIMER_RIGHT_3=237,20 PRIMER_LEFT_3_TM=60.152 PRIMER_RIGHT_3_TM=59.323 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=162 PRIMER_PAIR_4_PENALTY=0.837797 PRIMER_LEFT_4_PENALTY=0.151542 PRIMER_RIGHT_4_PENALTY=0.686256 PRIMER_LEFT_4_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_4_SEQUENCE=CGCATGTAGAGGTTCAGGTG PRIMER_LEFT_4=76,20 PRIMER_RIGHT_4=232,20 PRIMER_LEFT_4_TM=60.152 PRIMER_RIGHT_4_TM=59.314 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=157 PRIMER_PAIR_5_PENALTY=0.837797 PRIMER_LEFT_5_PENALTY=0.151542 PRIMER_RIGHT_5_PENALTY=0.686256 PRIMER_LEFT_5_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_5_SEQUENCE=CACGCATGTAGAGGTTCAGG PRIMER_LEFT_5=76,20 PRIMER_RIGHT_5=234,20 PRIMER_LEFT_5_TM=60.152 PRIMER_RIGHT_5_TM=59.314 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=55.000 PRIMER_LEFT_5_SELF_ANY=5.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_END_STABILITY=9.7000 PRIMER_RIGHT_5_END_STABILITY=8.2000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PAIR_5_PRODUCT_SIZE=159 PRIMER_PAIR_6_PENALTY=0.918041 PRIMER_LEFT_6_PENALTY=0.151542 PRIMER_RIGHT_6_PENALTY=0.766499 PRIMER_LEFT_6_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_6_SEQUENCE=TGAACATGTGCACGCATGTA PRIMER_LEFT_6=76,20 PRIMER_RIGHT_6=244,20 PRIMER_LEFT_6_TM=60.152 PRIMER_RIGHT_6_TM=60.766 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=45.000 PRIMER_LEFT_6_SELF_ANY=5.00 PRIMER_RIGHT_6_SELF_ANY=8.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=6.00 PRIMER_LEFT_6_END_STABILITY=9.7000 PRIMER_RIGHT_6_END_STABILITY=5.6000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=2.00 PRIMER_PAIR_6_PRODUCT_SIZE=169 PRIMER_PAIR_7_PENALTY=0.956229 PRIMER_LEFT_7_PENALTY=0.151542 PRIMER_RIGHT_7_PENALTY=0.804687 PRIMER_LEFT_7_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_7_SEQUENCE=ATGTGCACGCATGTAGAGGT PRIMER_LEFT_7=76,20 PRIMER_RIGHT_7=239,20 PRIMER_LEFT_7_TM=60.152 PRIMER_RIGHT_7_TM=59.195 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=50.000 PRIMER_LEFT_7_SELF_ANY=5.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=3.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=7.6000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PAIR_7_PRODUCT_SIZE=164 PRIMER_PAIR_8_PENALTY=1.016725 PRIMER_LEFT_8_PENALTY=0.683672 PRIMER_RIGHT_8_PENALTY=0.333053 PRIMER_LEFT_8_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_8_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_8=88,20 PRIMER_RIGHT_8=238,20 PRIMER_LEFT_8_TM=59.316 PRIMER_RIGHT_8_TM=60.333 PRIMER_LEFT_8_GC_PERCENT=45.000 PRIMER_RIGHT_8_GC_PERCENT=50.000 PRIMER_LEFT_8_SELF_ANY=5.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=5.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_LEFT_8_END_STABILITY=9.7000 PRIMER_RIGHT_8_END_STABILITY=7.9000 PRIMER_PAIR_8_COMPL_ANY=4.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PAIR_8_PRODUCT_SIZE=151 PRIMER_PAIR_9_PENALTY=1.108771 PRIMER_LEFT_9_PENALTY=0.775718 PRIMER_RIGHT_9_PENALTY=0.333053 PRIMER_LEFT_9_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_9_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_9=87,20 PRIMER_RIGHT_9=238,20 PRIMER_LEFT_9_TM=60.776 PRIMER_RIGHT_9_TM=60.333 PRIMER_LEFT_9_GC_PERCENT=45.000 PRIMER_RIGHT_9_GC_PERCENT=50.000 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=0.00 PRIMER_LEFT_9_END_STABILITY=9.4000 PRIMER_RIGHT_9_END_STABILITY=7.9000 PRIMER_PAIR_9_COMPL_ANY=4.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=152 PRIMER_PAIR_10_PENALTY=1.108771 PRIMER_LEFT_10_PENALTY=0.775718 PRIMER_RIGHT_10_PENALTY=0.333053 PRIMER_LEFT_10_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_10_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_10=86,20 PRIMER_RIGHT_10=238,20 PRIMER_LEFT_10_TM=60.776 PRIMER_RIGHT_10_TM=60.333 PRIMER_LEFT_10_GC_PERCENT=50.000 PRIMER_RIGHT_10_GC_PERCENT=50.000 PRIMER_LEFT_10_SELF_ANY=5.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_END_STABILITY=9.7000 PRIMER_RIGHT_10_END_STABILITY=7.9000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PAIR_10_PRODUCT_SIZE=153 PRIMER_PAIR_11_PENALTY=1.213321 PRIMER_LEFT_11_PENALTY=0.683672 PRIMER_RIGHT_11_PENALTY=0.529649 PRIMER_LEFT_11_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_11_SEQUENCE=GCACGCATGTAGAGGTTCAG PRIMER_LEFT_11=88,20 PRIMER_RIGHT_11=235,20 PRIMER_LEFT_11_TM=59.316 PRIMER_RIGHT_11_TM=59.470 PRIMER_LEFT_11_GC_PERCENT=45.000 PRIMER_RIGHT_11_GC_PERCENT=55.000 PRIMER_LEFT_11_SELF_ANY=5.00 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=5.00 PRIMER_RIGHT_11_SELF_END=1.00 PRIMER_LEFT_11_END_STABILITY=9.7000 PRIMER_RIGHT_11_END_STABILITY=7.0000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=1.00 PRIMER_PAIR_11_PRODUCT_SIZE=148 PRIMER_PAIR_12_PENALTY=1.277825 PRIMER_LEFT_12_PENALTY=0.151542 PRIMER_RIGHT_12_PENALTY=1.126283 PRIMER_LEFT_12_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_12_SEQUENCE=GCATGTAGAGGTTCAGGTGGA PRIMER_LEFT_12=76,20 PRIMER_RIGHT_12=231,21 PRIMER_LEFT_12_TM=60.152 PRIMER_RIGHT_12_TM=60.126 PRIMER_LEFT_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_GC_PERCENT=52.381 PRIMER_LEFT_12_SELF_ANY=5.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=3.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_END_STABILITY=9.7000 PRIMER_RIGHT_12_END_STABILITY=7.9000 PRIMER_PAIR_12_COMPL_ANY=4.00 PRIMER_PAIR_12_COMPL_END=0.00 PRIMER_PAIR_12_PRODUCT_SIZE=156 PRIMER_PAIR_13_PENALTY=1.305367 PRIMER_LEFT_13_PENALTY=0.775718 PRIMER_RIGHT_13_PENALTY=0.529649 PRIMER_LEFT_13_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_13_SEQUENCE=GCACGCATGTAGAGGTTCAG PRIMER_LEFT_13=87,20 PRIMER_RIGHT_13=235,20 PRIMER_LEFT_13_TM=60.776 PRIMER_RIGHT_13_TM=59.470 PRIMER_LEFT_13_GC_PERCENT=45.000 PRIMER_RIGHT_13_GC_PERCENT=55.000 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=3.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_LEFT_13_END_STABILITY=9.4000 PRIMER_RIGHT_13_END_STABILITY=7.0000 PRIMER_PAIR_13_COMPL_ANY=4.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PAIR_13_PRODUCT_SIZE=149 PRIMER_PAIR_14_PENALTY=1.305367 PRIMER_LEFT_14_PENALTY=0.775718 PRIMER_RIGHT_14_PENALTY=0.529649 PRIMER_LEFT_14_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_14_SEQUENCE=GCACGCATGTAGAGGTTCAG PRIMER_LEFT_14=86,20 PRIMER_RIGHT_14=235,20 PRIMER_LEFT_14_TM=60.776 PRIMER_RIGHT_14_TM=59.470 PRIMER_LEFT_14_GC_PERCENT=50.000 PRIMER_RIGHT_14_GC_PERCENT=55.000 PRIMER_LEFT_14_SELF_ANY=5.00 PRIMER_RIGHT_14_SELF_ANY=4.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_END_STABILITY=9.7000 PRIMER_RIGHT_14_END_STABILITY=7.0000 PRIMER_PAIR_14_COMPL_ANY=4.00 PRIMER_PAIR_14_COMPL_END=1.00 PRIMER_PAIR_14_PRODUCT_SIZE=150 PRIMER_PAIR_15_PENALTY=1.321863 PRIMER_LEFT_15_PENALTY=0.683672 PRIMER_RIGHT_15_PENALTY=0.638191 PRIMER_LEFT_15_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_15_SEQUENCE=AGAGGTTCAGGTGGAGAGGG PRIMER_LEFT_15=88,20 PRIMER_RIGHT_15=225,20 PRIMER_LEFT_15_TM=59.316 PRIMER_RIGHT_15_TM=60.638 PRIMER_LEFT_15_GC_PERCENT=45.000 PRIMER_RIGHT_15_GC_PERCENT=60.000 PRIMER_LEFT_15_SELF_ANY=5.00 PRIMER_RIGHT_15_SELF_ANY=2.00 PRIMER_LEFT_15_SELF_END=5.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_END_STABILITY=9.7000 PRIMER_RIGHT_15_END_STABILITY=9.4000 PRIMER_PAIR_15_COMPL_ANY=2.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PAIR_15_PRODUCT_SIZE=138 PRIMER_PAIR_16_PENALTY=1.337484 PRIMER_LEFT_16_PENALTY=0.151542 PRIMER_RIGHT_16_PENALTY=1.185942 PRIMER_LEFT_16_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_16_SEQUENCE=ACGCATGTAGAGGTTCAGGTG PRIMER_LEFT_16=76,20 PRIMER_RIGHT_16=233,21 PRIMER_LEFT_16_TM=60.152 PRIMER_RIGHT_16_TM=60.186 PRIMER_LEFT_16_GC_PERCENT=50.000 PRIMER_RIGHT_16_GC_PERCENT=52.381 PRIMER_LEFT_16_SELF_ANY=5.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=2.00 PRIMER_LEFT_16_END_STABILITY=9.7000 PRIMER_RIGHT_16_END_STABILITY=7.9000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PAIR_16_PRODUCT_SIZE=158 PRIMER_PAIR_17_PENALTY=1.337484 PRIMER_LEFT_17_PENALTY=0.151542 PRIMER_RIGHT_17_PENALTY=1.185942 PRIMER_LEFT_17_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_17_SEQUENCE=CACGCATGTAGAGGTTCAGGT PRIMER_LEFT_17=76,20 PRIMER_RIGHT_17=234,21 PRIMER_LEFT_17_TM=60.152 PRIMER_RIGHT_17_TM=60.186 PRIMER_LEFT_17_GC_PERCENT=50.000 PRIMER_RIGHT_17_GC_PERCENT=52.381 PRIMER_LEFT_17_SELF_ANY=5.00 PRIMER_RIGHT_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_END_STABILITY=9.7000 PRIMER_RIGHT_17_END_STABILITY=7.9000 PRIMER_PAIR_17_COMPL_ANY=4.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PAIR_17_PRODUCT_SIZE=159 PRIMER_PAIR_18_PENALTY=1.348445 PRIMER_LEFT_18_PENALTY=0.151542 PRIMER_RIGHT_18_PENALTY=1.196903 PRIMER_LEFT_18_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_18_SEQUENCE=AACATGTGCACGCATGTAGAG PRIMER_LEFT_18=76,20 PRIMER_RIGHT_18=242,21 PRIMER_LEFT_18_TM=60.152 PRIMER_RIGHT_18_TM=59.803 PRIMER_LEFT_18_GC_PERCENT=50.000 PRIMER_RIGHT_18_GC_PERCENT=47.619 PRIMER_LEFT_18_SELF_ANY=5.00 PRIMER_RIGHT_18_SELF_ANY=8.00 PRIMER_LEFT_18_SELF_END=3.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_END_STABILITY=9.7000 PRIMER_RIGHT_18_END_STABILITY=5.7000 PRIMER_PAIR_18_COMPL_ANY=4.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PAIR_18_PRODUCT_SIZE=167 PRIMER_PAIR_19_PENALTY=1.355841 PRIMER_LEFT_19_PENALTY=1.022788 PRIMER_RIGHT_19_PENALTY=0.333053 PRIMER_LEFT_19_SEQUENCE=TTTGGGTACAAACTGACAGGC PRIMER_RIGHT_19_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_19=74,21 PRIMER_RIGHT_19=238,20 PRIMER_LEFT_19_TM=60.023 PRIMER_RIGHT_19_TM=60.333 PRIMER_LEFT_19_GC_PERCENT=47.619 PRIMER_RIGHT_19_GC_PERCENT=50.000 PRIMER_LEFT_19_SELF_ANY=5.00 PRIMER_RIGHT_19_SELF_ANY=6.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_END_STABILITY=9.7000 PRIMER_RIGHT_19_END_STABILITY=7.9000 PRIMER_PAIR_19_COMPL_ANY=4.00 PRIMER_PAIR_19_COMPL_END=4.00 PRIMER_PAIR_19_PRODUCT_SIZE=165 PRIMER_PAIR_20_PENALTY=1.360619 PRIMER_LEFT_20_PENALTY=0.683672 PRIMER_RIGHT_20_PENALTY=0.676947 PRIMER_LEFT_20_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_20_SEQUENCE=GTGCACGCATGTAGAGGTTC PRIMER_LEFT_20=88,20 PRIMER_RIGHT_20=237,20 PRIMER_LEFT_20_TM=59.316 PRIMER_RIGHT_20_TM=59.323 PRIMER_LEFT_20_GC_PERCENT=45.000 PRIMER_RIGHT_20_GC_PERCENT=55.000 PRIMER_LEFT_20_SELF_ANY=5.00 PRIMER_RIGHT_20_SELF_ANY=6.00 PRIMER_LEFT_20_SELF_END=5.00 PRIMER_RIGHT_20_SELF_END=1.00 PRIMER_LEFT_20_END_STABILITY=9.7000 PRIMER_RIGHT_20_END_STABILITY=7.9000 PRIMER_PAIR_20_COMPL_ANY=4.00 PRIMER_PAIR_20_COMPL_END=0.00 PRIMER_PAIR_20_PRODUCT_SIZE=150 PRIMER_PAIR_21_PENALTY=1.369928 PRIMER_LEFT_21_PENALTY=0.683672 PRIMER_RIGHT_21_PENALTY=0.686256 PRIMER_LEFT_21_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_21_SEQUENCE=CGCATGTAGAGGTTCAGGTG PRIMER_LEFT_21=88,20 PRIMER_RIGHT_21=232,20 PRIMER_LEFT_21_TM=59.316 PRIMER_RIGHT_21_TM=59.314 PRIMER_LEFT_21_GC_PERCENT=45.000 PRIMER_RIGHT_21_GC_PERCENT=55.000 PRIMER_LEFT_21_SELF_ANY=5.00 PRIMER_RIGHT_21_SELF_ANY=4.00 PRIMER_LEFT_21_SELF_END=5.00 PRIMER_RIGHT_21_SELF_END=2.00 PRIMER_LEFT_21_END_STABILITY=9.7000 PRIMER_RIGHT_21_END_STABILITY=7.9000 PRIMER_PAIR_21_COMPL_ANY=4.00 PRIMER_PAIR_21_COMPL_END=0.00 PRIMER_PAIR_21_PRODUCT_SIZE=145 PRIMER_PAIR_22_PENALTY=1.369928 PRIMER_LEFT_22_PENALTY=0.683672 PRIMER_RIGHT_22_PENALTY=0.686256 PRIMER_LEFT_22_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_22_SEQUENCE=CACGCATGTAGAGGTTCAGG PRIMER_LEFT_22=88,20 PRIMER_RIGHT_22=234,20 PRIMER_LEFT_22_TM=59.316 PRIMER_RIGHT_22_TM=59.314 PRIMER_LEFT_22_GC_PERCENT=45.000 PRIMER_RIGHT_22_GC_PERCENT=55.000 PRIMER_LEFT_22_SELF_ANY=5.00 PRIMER_RIGHT_22_SELF_ANY=4.00 PRIMER_LEFT_22_SELF_END=5.00 PRIMER_RIGHT_22_SELF_END=0.00 PRIMER_LEFT_22_END_STABILITY=9.7000 PRIMER_RIGHT_22_END_STABILITY=8.2000 PRIMER_PAIR_22_COMPL_ANY=4.00 PRIMER_PAIR_22_COMPL_END=1.00 PRIMER_PAIR_22_PRODUCT_SIZE=147 PRIMER_PAIR_23_PENALTY=1.413909 PRIMER_LEFT_23_PENALTY=0.775718 PRIMER_RIGHT_23_PENALTY=0.638191 PRIMER_LEFT_23_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_23_SEQUENCE=AGAGGTTCAGGTGGAGAGGG PRIMER_LEFT_23=87,20 PRIMER_RIGHT_23=225,20 PRIMER_LEFT_23_TM=60.776 PRIMER_RIGHT_23_TM=60.638 PRIMER_LEFT_23_GC_PERCENT=45.000 PRIMER_RIGHT_23_GC_PERCENT=60.000 PRIMER_LEFT_23_SELF_ANY=4.00 PRIMER_RIGHT_23_SELF_ANY=2.00 PRIMER_LEFT_23_SELF_END=3.00 PRIMER_RIGHT_23_SELF_END=0.00 PRIMER_LEFT_23_END_STABILITY=9.4000 PRIMER_RIGHT_23_END_STABILITY=9.4000 PRIMER_PAIR_23_COMPL_ANY=3.00 PRIMER_PAIR_23_COMPL_END=0.00 PRIMER_PAIR_23_PRODUCT_SIZE=139 PRIMER_PAIR_24_PENALTY=1.413909 PRIMER_LEFT_24_PENALTY=0.775718 PRIMER_RIGHT_24_PENALTY=0.638191 PRIMER_LEFT_24_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_24_SEQUENCE=AGAGGTTCAGGTGGAGAGGG PRIMER_LEFT_24=86,20 PRIMER_RIGHT_24=225,20 PRIMER_LEFT_24_TM=60.776 PRIMER_RIGHT_24_TM=60.638 PRIMER_LEFT_24_GC_PERCENT=50.000 PRIMER_RIGHT_24_GC_PERCENT=60.000 PRIMER_LEFT_24_SELF_ANY=5.00 PRIMER_RIGHT_24_SELF_ANY=2.00 PRIMER_LEFT_24_SELF_END=2.00 PRIMER_RIGHT_24_SELF_END=0.00 PRIMER_LEFT_24_END_STABILITY=9.7000 PRIMER_RIGHT_24_END_STABILITY=9.4000 PRIMER_PAIR_24_COMPL_ANY=4.00 PRIMER_PAIR_24_COMPL_END=1.00 PRIMER_PAIR_24_PRODUCT_SIZE=140 PRIMER_PAIR_25_PENALTY=1.417827 PRIMER_LEFT_25_PENALTY=0.151542 PRIMER_RIGHT_25_PENALTY=1.266285 PRIMER_LEFT_25_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_25_SEQUENCE=AACATGTGCACGCATGTAGA PRIMER_LEFT_25=76,20 PRIMER_RIGHT_25=242,20 PRIMER_LEFT_25_TM=60.152 PRIMER_RIGHT_25_TM=58.734 PRIMER_LEFT_25_GC_PERCENT=50.000 PRIMER_RIGHT_25_GC_PERCENT=45.000 PRIMER_LEFT_25_SELF_ANY=5.00 PRIMER_RIGHT_25_SELF_ANY=8.00 PRIMER_LEFT_25_SELF_END=3.00 PRIMER_RIGHT_25_SELF_END=4.00 PRIMER_LEFT_25_END_STABILITY=9.7000 PRIMER_RIGHT_25_END_STABILITY=5.4000 PRIMER_PAIR_25_COMPL_ANY=4.00 PRIMER_PAIR_25_COMPL_END=2.00 PRIMER_PAIR_25_PRODUCT_SIZE=167 PRIMER_PAIR_26_PENALTY=1.417827 PRIMER_LEFT_26_PENALTY=0.151542 PRIMER_RIGHT_26_PENALTY=1.266285 PRIMER_LEFT_26_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_26_SEQUENCE=GAACATGTGCACGCATGTAG PRIMER_LEFT_26=76,20 PRIMER_RIGHT_26=243,20 PRIMER_LEFT_26_TM=60.152 PRIMER_RIGHT_26_TM=58.734 PRIMER_LEFT_26_GC_PERCENT=50.000 PRIMER_RIGHT_26_GC_PERCENT=50.000 PRIMER_LEFT_26_SELF_ANY=5.00 PRIMER_RIGHT_26_SELF_ANY=8.00 PRIMER_LEFT_26_SELF_END=3.00 PRIMER_RIGHT_26_SELF_END=4.00 PRIMER_LEFT_26_END_STABILITY=9.7000 PRIMER_RIGHT_26_END_STABILITY=5.7000 PRIMER_PAIR_26_COMPL_ANY=4.00 PRIMER_PAIR_26_COMPL_END=3.00 PRIMER_PAIR_26_PRODUCT_SIZE=168 PRIMER_PAIR_27_PENALTY=1.450171 PRIMER_LEFT_27_PENALTY=0.683672 PRIMER_RIGHT_27_PENALTY=0.766499 PRIMER_LEFT_27_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_27_SEQUENCE=TGAACATGTGCACGCATGTA PRIMER_LEFT_27=88,20 PRIMER_RIGHT_27=244,20 PRIMER_LEFT_27_TM=59.316 PRIMER_RIGHT_27_TM=60.766 PRIMER_LEFT_27_GC_PERCENT=45.000 PRIMER_RIGHT_27_GC_PERCENT=45.000 PRIMER_LEFT_27_SELF_ANY=5.00 PRIMER_RIGHT_27_SELF_ANY=8.00 PRIMER_LEFT_27_SELF_END=5.00 PRIMER_RIGHT_27_SELF_END=6.00 PRIMER_LEFT_27_END_STABILITY=9.7000 PRIMER_RIGHT_27_END_STABILITY=5.6000 PRIMER_PAIR_27_COMPL_ANY=5.00 PRIMER_PAIR_27_COMPL_END=1.00 PRIMER_PAIR_27_PRODUCT_SIZE=157 PRIMER_PAIR_28_PENALTY=1.452665 PRIMER_LEFT_28_PENALTY=0.775718 PRIMER_RIGHT_28_PENALTY=0.676947 PRIMER_LEFT_28_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_28_SEQUENCE=GTGCACGCATGTAGAGGTTC PRIMER_LEFT_28=87,20 PRIMER_RIGHT_28=237,20 PRIMER_LEFT_28_TM=60.776 PRIMER_RIGHT_28_TM=59.323 PRIMER_LEFT_28_GC_PERCENT=45.000 PRIMER_RIGHT_28_GC_PERCENT=55.000 PRIMER_LEFT_28_SELF_ANY=4.00 PRIMER_RIGHT_28_SELF_ANY=6.00 PRIMER_LEFT_28_SELF_END=3.00 PRIMER_RIGHT_28_SELF_END=1.00 PRIMER_LEFT_28_END_STABILITY=9.4000 PRIMER_RIGHT_28_END_STABILITY=7.9000 PRIMER_PAIR_28_COMPL_ANY=4.00 PRIMER_PAIR_28_COMPL_END=0.00 PRIMER_PAIR_28_PRODUCT_SIZE=151 PRIMER_PAIR_29_PENALTY=1.452665 PRIMER_LEFT_29_PENALTY=0.775718 PRIMER_RIGHT_29_PENALTY=0.676947 PRIMER_LEFT_29_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_29_SEQUENCE=GTGCACGCATGTAGAGGTTC PRIMER_LEFT_29=86,20 PRIMER_RIGHT_29=237,20 PRIMER_LEFT_29_TM=60.776 PRIMER_RIGHT_29_TM=59.323 PRIMER_LEFT_29_GC_PERCENT=50.000 PRIMER_RIGHT_29_GC_PERCENT=55.000 PRIMER_LEFT_29_SELF_ANY=5.00 PRIMER_RIGHT_29_SELF_ANY=6.00 PRIMER_LEFT_29_SELF_END=2.00 PRIMER_RIGHT_29_SELF_END=1.00 PRIMER_LEFT_29_END_STABILITY=9.7000 PRIMER_RIGHT_29_END_STABILITY=7.9000 PRIMER_PAIR_29_COMPL_ANY=4.00 PRIMER_PAIR_29_COMPL_END=0.00 PRIMER_PAIR_29_PRODUCT_SIZE=152 PRIMER_PAIR_30_PENALTY=1.461974 PRIMER_LEFT_30_PENALTY=0.775718 PRIMER_RIGHT_30_PENALTY=0.686256 PRIMER_LEFT_30_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_30_SEQUENCE=CGCATGTAGAGGTTCAGGTG PRIMER_LEFT_30=87,20 PRIMER_RIGHT_30=232,20 PRIMER_LEFT_30_TM=60.776 PRIMER_RIGHT_30_TM=59.314 PRIMER_LEFT_30_GC_PERCENT=45.000 PRIMER_RIGHT_30_GC_PERCENT=55.000 PRIMER_LEFT_30_SELF_ANY=4.00 PRIMER_RIGHT_30_SELF_ANY=4.00 PRIMER_LEFT_30_SELF_END=3.00 PRIMER_RIGHT_30_SELF_END=2.00 PRIMER_LEFT_30_END_STABILITY=9.4000 PRIMER_RIGHT_30_END_STABILITY=7.9000 PRIMER_PAIR_30_COMPL_ANY=4.00 PRIMER_PAIR_30_COMPL_END=0.00 PRIMER_PAIR_30_PRODUCT_SIZE=146 PRIMER_PAIR_31_PENALTY=1.461974 PRIMER_LEFT_31_PENALTY=0.775718 PRIMER_RIGHT_31_PENALTY=0.686256 PRIMER_LEFT_31_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_31_SEQUENCE=CACGCATGTAGAGGTTCAGG PRIMER_LEFT_31=87,20 PRIMER_RIGHT_31=234,20 PRIMER_LEFT_31_TM=60.776 PRIMER_RIGHT_31_TM=59.314 PRIMER_LEFT_31_GC_PERCENT=45.000 PRIMER_RIGHT_31_GC_PERCENT=55.000 PRIMER_LEFT_31_SELF_ANY=4.00 PRIMER_RIGHT_31_SELF_ANY=4.00 PRIMER_LEFT_31_SELF_END=3.00 PRIMER_RIGHT_31_SELF_END=0.00 PRIMER_LEFT_31_END_STABILITY=9.4000 PRIMER_RIGHT_31_END_STABILITY=8.2000 PRIMER_PAIR_31_COMPL_ANY=4.00 PRIMER_PAIR_31_COMPL_END=1.00 PRIMER_PAIR_31_PRODUCT_SIZE=148 PRIMER_PAIR_32_PENALTY=1.461974 PRIMER_LEFT_32_PENALTY=0.775718 PRIMER_RIGHT_32_PENALTY=0.686256 PRIMER_LEFT_32_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_32_SEQUENCE=CGCATGTAGAGGTTCAGGTG PRIMER_LEFT_32=86,20 PRIMER_RIGHT_32=232,20 PRIMER_LEFT_32_TM=60.776 PRIMER_RIGHT_32_TM=59.314 PRIMER_LEFT_32_GC_PERCENT=50.000 PRIMER_RIGHT_32_GC_PERCENT=55.000 PRIMER_LEFT_32_SELF_ANY=5.00 PRIMER_RIGHT_32_SELF_ANY=4.00 PRIMER_LEFT_32_SELF_END=2.00 PRIMER_RIGHT_32_SELF_END=2.00 PRIMER_LEFT_32_END_STABILITY=9.7000 PRIMER_RIGHT_32_END_STABILITY=7.9000 PRIMER_PAIR_32_COMPL_ANY=4.00 PRIMER_PAIR_32_COMPL_END=1.00 PRIMER_PAIR_32_PRODUCT_SIZE=147 PRIMER_PAIR_33_PENALTY=1.461974 PRIMER_LEFT_33_PENALTY=0.775718 PRIMER_RIGHT_33_PENALTY=0.686256 PRIMER_LEFT_33_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_33_SEQUENCE=CACGCATGTAGAGGTTCAGG PRIMER_LEFT_33=86,20 PRIMER_RIGHT_33=234,20 PRIMER_LEFT_33_TM=60.776 PRIMER_RIGHT_33_TM=59.314 PRIMER_LEFT_33_GC_PERCENT=50.000 PRIMER_RIGHT_33_GC_PERCENT=55.000 PRIMER_LEFT_33_SELF_ANY=5.00 PRIMER_RIGHT_33_SELF_ANY=4.00 PRIMER_LEFT_33_SELF_END=2.00 PRIMER_RIGHT_33_SELF_END=0.00 PRIMER_LEFT_33_END_STABILITY=9.7000 PRIMER_RIGHT_33_END_STABILITY=8.2000 PRIMER_PAIR_33_COMPL_ANY=4.00 PRIMER_PAIR_33_COMPL_END=1.00 PRIMER_PAIR_33_PRODUCT_SIZE=149 PRIMER_PAIR_34_PENALTY=1.469743 PRIMER_LEFT_34_PENALTY=0.151542 PRIMER_RIGHT_34_PENALTY=1.318201 PRIMER_LEFT_34_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_34_SEQUENCE=CATGTGCACGCATGTAGAGG PRIMER_LEFT_34=76,20 PRIMER_RIGHT_34=240,20 PRIMER_LEFT_34_TM=60.152 PRIMER_RIGHT_34_TM=61.318 PRIMER_LEFT_34_GC_PERCENT=50.000 PRIMER_RIGHT_34_GC_PERCENT=55.000 PRIMER_LEFT_34_SELF_ANY=5.00 PRIMER_RIGHT_34_SELF_ANY=6.00 PRIMER_LEFT_34_SELF_END=3.00 PRIMER_RIGHT_34_SELF_END=4.00 PRIMER_LEFT_34_END_STABILITY=9.7000 PRIMER_RIGHT_34_END_STABILITY=7.9000 PRIMER_PAIR_34_COMPL_ANY=4.00 PRIMER_PAIR_34_COMPL_END=1.00 PRIMER_PAIR_34_PRODUCT_SIZE=165 PRIMER_PAIR_35_PENALTY=1.488359 PRIMER_LEFT_35_PENALTY=0.683672 PRIMER_RIGHT_35_PENALTY=0.804687 PRIMER_LEFT_35_SEQUENCE=GACAGGCAATTTCAAGGCTT PRIMER_RIGHT_35_SEQUENCE=ATGTGCACGCATGTAGAGGT PRIMER_LEFT_35=88,20 PRIMER_RIGHT_35=239,20 PRIMER_LEFT_35_TM=59.316 PRIMER_RIGHT_35_TM=59.195 PRIMER_LEFT_35_GC_PERCENT=45.000 PRIMER_RIGHT_35_GC_PERCENT=50.000 PRIMER_LEFT_35_SELF_ANY=5.00 PRIMER_RIGHT_35_SELF_ANY=6.00 PRIMER_LEFT_35_SELF_END=5.00 PRIMER_RIGHT_35_SELF_END=0.00 PRIMER_LEFT_35_END_STABILITY=9.7000 PRIMER_RIGHT_35_END_STABILITY=7.6000 PRIMER_PAIR_35_COMPL_ANY=4.00 PRIMER_PAIR_35_COMPL_END=0.00 PRIMER_PAIR_35_PRODUCT_SIZE=152 PRIMER_PAIR_36_PENALTY=1.529357 PRIMER_LEFT_36_PENALTY=0.151542 PRIMER_RIGHT_36_PENALTY=1.377815 PRIMER_LEFT_36_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_36_SEQUENCE=GAGGTTCAGGTGGAGAGGG PRIMER_LEFT_36=76,20 PRIMER_RIGHT_36=224,19 PRIMER_LEFT_36_TM=60.152 PRIMER_RIGHT_36_TM=59.622 PRIMER_LEFT_36_GC_PERCENT=50.000 PRIMER_RIGHT_36_GC_PERCENT=63.158 PRIMER_LEFT_36_SELF_ANY=5.00 PRIMER_RIGHT_36_SELF_ANY=2.00 PRIMER_LEFT_36_SELF_END=3.00 PRIMER_RIGHT_36_SELF_END=0.00 PRIMER_LEFT_36_END_STABILITY=9.7000 PRIMER_RIGHT_36_END_STABILITY=9.4000 PRIMER_PAIR_36_COMPL_ANY=4.00 PRIMER_PAIR_36_COMPL_END=0.00 PRIMER_PAIR_36_PRODUCT_SIZE=149 PRIMER_PAIR_37_PENALTY=1.542217 PRIMER_LEFT_37_PENALTY=0.775718 PRIMER_RIGHT_37_PENALTY=0.766499 PRIMER_LEFT_37_SEQUENCE=TGACAGGCAATTTCAAGGCT PRIMER_RIGHT_37_SEQUENCE=TGAACATGTGCACGCATGTA PRIMER_LEFT_37=87,20 PRIMER_RIGHT_37=244,20 PRIMER_LEFT_37_TM=60.776 PRIMER_RIGHT_37_TM=60.766 PRIMER_LEFT_37_GC_PERCENT=45.000 PRIMER_RIGHT_37_GC_PERCENT=45.000 PRIMER_LEFT_37_SELF_ANY=4.00 PRIMER_RIGHT_37_SELF_ANY=8.00 PRIMER_LEFT_37_SELF_END=3.00 PRIMER_RIGHT_37_SELF_END=6.00 PRIMER_LEFT_37_END_STABILITY=9.4000 PRIMER_RIGHT_37_END_STABILITY=5.6000 PRIMER_PAIR_37_COMPL_ANY=5.00 PRIMER_PAIR_37_COMPL_END=1.00 PRIMER_PAIR_37_PRODUCT_SIZE=158 PRIMER_PAIR_38_PENALTY=1.542217 PRIMER_LEFT_38_PENALTY=0.775718 PRIMER_RIGHT_38_PENALTY=0.766499 PRIMER_LEFT_38_SEQUENCE=CTGACAGGCAATTTCAAGGC PRIMER_RIGHT_38_SEQUENCE=TGAACATGTGCACGCATGTA PRIMER_LEFT_38=86,20 PRIMER_RIGHT_38=244,20 PRIMER_LEFT_38_TM=60.776 PRIMER_RIGHT_38_TM=60.766 PRIMER_LEFT_38_GC_PERCENT=50.000 PRIMER_RIGHT_38_GC_PERCENT=45.000 PRIMER_LEFT_38_SELF_ANY=5.00 PRIMER_RIGHT_38_SELF_ANY=8.00 PRIMER_LEFT_38_SELF_END=2.00 PRIMER_RIGHT_38_SELF_END=6.00 PRIMER_LEFT_38_END_STABILITY=9.7000 PRIMER_RIGHT_38_END_STABILITY=5.6000 PRIMER_PAIR_38_COMPL_ANY=5.00 PRIMER_PAIR_38_COMPL_END=1.00 PRIMER_PAIR_38_PRODUCT_SIZE=159 PRIMER_PAIR_39_PENALTY=1.543141 PRIMER_LEFT_39_PENALTY=1.210088 PRIMER_RIGHT_39_PENALTY=0.333053 PRIMER_LEFT_39_SEQUENCE=TGACAGGCAATTTCAAGGC PRIMER_RIGHT_39_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_39=87,19 PRIMER_RIGHT_39=238,20 PRIMER_LEFT_39_TM=59.790 PRIMER_RIGHT_39_TM=60.333 PRIMER_LEFT_39_GC_PERCENT=47.368 PRIMER_RIGHT_39_GC_PERCENT=50.000 PRIMER_LEFT_39_SELF_ANY=4.00 PRIMER_RIGHT_39_SELF_ANY=6.00 PRIMER_LEFT_39_SELF_END=2.00 PRIMER_RIGHT_39_SELF_END=0.00 PRIMER_LEFT_39_END_STABILITY=9.7000 PRIMER_RIGHT_39_END_STABILITY=7.9000 PRIMER_PAIR_39_COMPL_ANY=4.00 PRIMER_PAIR_39_COMPL_END=1.00 PRIMER_PAIR_39_PRODUCT_SIZE=152 = SEQUENCE_ID=08.RHAP34FE8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG SEQUENCE_INCLUDED_REGION=20,281 SEQUENCE_TARGET=119,62 PRIMER_LEFT_NUM_RETURNED=40 PRIMER_RIGHT_NUM_RETURNED=40 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=40 PRIMER_PAIR_0_PENALTY=0.218990 PRIMER_LEFT_0_PENALTY=0.201280 PRIMER_RIGHT_0_PENALTY=0.017711 PRIMER_LEFT_0_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_0_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_0=53,20 PRIMER_RIGHT_0=220,20 PRIMER_LEFT_0_TM=59.799 PRIMER_RIGHT_0_TM=59.982 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=168 PRIMER_PAIR_1_PENALTY=0.267700 PRIMER_LEFT_1_PENALTY=0.249989 PRIMER_RIGHT_1_PENALTY=0.017711 PRIMER_LEFT_1_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_1_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_1=83,20 PRIMER_RIGHT_1=220,20 PRIMER_LEFT_1_TM=59.750 PRIMER_RIGHT_1_TM=59.982 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=138 PRIMER_PAIR_2_PENALTY=0.357340 PRIMER_LEFT_2_PENALTY=0.201280 PRIMER_RIGHT_2_PENALTY=0.156061 PRIMER_LEFT_2_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_2_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_2=53,20 PRIMER_RIGHT_2=218,20 PRIMER_LEFT_2_TM=59.799 PRIMER_RIGHT_2_TM=59.844 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=166 PRIMER_PAIR_3_PENALTY=0.406050 PRIMER_LEFT_3_PENALTY=0.249989 PRIMER_RIGHT_3_PENALTY=0.156061 PRIMER_LEFT_3_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_3_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_3=83,20 PRIMER_RIGHT_3=218,20 PRIMER_LEFT_3_TM=59.750 PRIMER_RIGHT_3_TM=59.844 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=136 PRIMER_PAIR_4_PENALTY=0.440598 PRIMER_LEFT_4_PENALTY=0.422888 PRIMER_RIGHT_4_PENALTY=0.017711 PRIMER_LEFT_4_SEQUENCE=GCTTTTTCCCCTTAGGACCA PRIMER_RIGHT_4_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_4=49,20 PRIMER_RIGHT_4=220,20 PRIMER_LEFT_4_TM=60.423 PRIMER_RIGHT_4_TM=59.982 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=172 PRIMER_PAIR_5_PENALTY=0.510151 PRIMER_LEFT_5_PENALTY=0.201280 PRIMER_RIGHT_5_PENALTY=0.308871 PRIMER_LEFT_5_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_5_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_5=53,20 PRIMER_RIGHT_5=217,20 PRIMER_LEFT_5_TM=59.799 PRIMER_RIGHT_5_TM=59.691 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=55.000 PRIMER_LEFT_5_SELF_ANY=7.00 PRIMER_RIGHT_5_SELF_ANY=5.00 PRIMER_LEFT_5_SELF_END=5.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_END_STABILITY=7.9000 PRIMER_RIGHT_5_END_STABILITY=9.4000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PAIR_5_PRODUCT_SIZE=165 PRIMER_PAIR_6_PENALTY=0.543290 PRIMER_LEFT_6_PENALTY=0.525579 PRIMER_RIGHT_6_PENALTY=0.017711 PRIMER_LEFT_6_SEQUENCE=GGACCACGGATTATCCTTCC PRIMER_RIGHT_6_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_6=82,20 PRIMER_RIGHT_6=220,20 PRIMER_LEFT_6_TM=60.526 PRIMER_RIGHT_6_TM=59.982 PRIMER_LEFT_6_GC_PERCENT=55.000 PRIMER_RIGHT_6_GC_PERCENT=55.000 PRIMER_LEFT_6_SELF_ANY=7.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=4.00 PRIMER_LEFT_6_END_STABILITY=8.2000 PRIMER_RIGHT_6_END_STABILITY=9.7000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=2.00 PRIMER_PAIR_6_PRODUCT_SIZE=139 PRIMER_PAIR_7_PENALTY=0.558861 PRIMER_LEFT_7_PENALTY=0.249989 PRIMER_RIGHT_7_PENALTY=0.308871 PRIMER_LEFT_7_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_7_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_7=83,20 PRIMER_RIGHT_7=217,20 PRIMER_LEFT_7_TM=59.750 PRIMER_RIGHT_7_TM=59.691 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=55.000 PRIMER_LEFT_7_SELF_ANY=7.00 PRIMER_RIGHT_7_SELF_ANY=5.00 PRIMER_LEFT_7_SELF_END=1.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_END_STABILITY=8.5000 PRIMER_RIGHT_7_END_STABILITY=9.4000 PRIMER_PAIR_7_COMPL_ANY=5.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PAIR_7_PRODUCT_SIZE=135 PRIMER_PAIR_8_PENALTY=0.578948 PRIMER_LEFT_8_PENALTY=0.422888 PRIMER_RIGHT_8_PENALTY=0.156061 PRIMER_LEFT_8_SEQUENCE=GCTTTTTCCCCTTAGGACCA PRIMER_RIGHT_8_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_8=49,20 PRIMER_RIGHT_8=218,20 PRIMER_LEFT_8_TM=60.423 PRIMER_RIGHT_8_TM=59.844 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=55.000 PRIMER_LEFT_8_SELF_ANY=5.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_LEFT_8_SELF_END=1.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_END_STABILITY=7.9000 PRIMER_RIGHT_8_END_STABILITY=8.2000 PRIMER_PAIR_8_COMPL_ANY=4.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PAIR_8_PRODUCT_SIZE=170 PRIMER_PAIR_9_PENALTY=0.681640 PRIMER_LEFT_9_PENALTY=0.525579 PRIMER_RIGHT_9_PENALTY=0.156061 PRIMER_LEFT_9_SEQUENCE=GGACCACGGATTATCCTTCC PRIMER_RIGHT_9_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_9=82,20 PRIMER_RIGHT_9=218,20 PRIMER_LEFT_9_TM=60.526 PRIMER_RIGHT_9_TM=59.844 PRIMER_LEFT_9_GC_PERCENT=55.000 PRIMER_RIGHT_9_GC_PERCENT=55.000 PRIMER_LEFT_9_SELF_ANY=7.00 PRIMER_RIGHT_9_SELF_ANY=5.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_END_STABILITY=8.2000 PRIMER_RIGHT_9_END_STABILITY=8.2000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PAIR_9_PRODUCT_SIZE=137 PRIMER_PAIR_10_PENALTY=0.731759 PRIMER_LEFT_10_PENALTY=0.422888 PRIMER_RIGHT_10_PENALTY=0.308871 PRIMER_LEFT_10_SEQUENCE=GCTTTTTCCCCTTAGGACCA PRIMER_RIGHT_10_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_10=49,20 PRIMER_RIGHT_10=217,20 PRIMER_LEFT_10_TM=60.423 PRIMER_RIGHT_10_TM=59.691 PRIMER_LEFT_10_GC_PERCENT=50.000 PRIMER_RIGHT_10_GC_PERCENT=55.000 PRIMER_LEFT_10_SELF_ANY=5.00 PRIMER_RIGHT_10_SELF_ANY=5.00 PRIMER_LEFT_10_SELF_END=1.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_END_STABILITY=7.9000 PRIMER_RIGHT_10_END_STABILITY=9.4000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=3.00 PRIMER_PAIR_10_PRODUCT_SIZE=169 PRIMER_PAIR_11_PENALTY=0.834451 PRIMER_LEFT_11_PENALTY=0.525579 PRIMER_RIGHT_11_PENALTY=0.308871 PRIMER_LEFT_11_SEQUENCE=GGACCACGGATTATCCTTCC PRIMER_RIGHT_11_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_11=82,20 PRIMER_RIGHT_11=217,20 PRIMER_LEFT_11_TM=60.526 PRIMER_RIGHT_11_TM=59.691 PRIMER_LEFT_11_GC_PERCENT=55.000 PRIMER_RIGHT_11_GC_PERCENT=55.000 PRIMER_LEFT_11_SELF_ANY=7.00 PRIMER_RIGHT_11_SELF_ANY=5.00 PRIMER_LEFT_11_SELF_END=3.00 PRIMER_RIGHT_11_SELF_END=1.00 PRIMER_LEFT_11_END_STABILITY=8.2000 PRIMER_RIGHT_11_END_STABILITY=9.4000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PAIR_11_PRODUCT_SIZE=136 PRIMER_PAIR_12_PENALTY=0.970916 PRIMER_LEFT_12_PENALTY=0.201280 PRIMER_RIGHT_12_PENALTY=0.769636 PRIMER_LEFT_12_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_12_SEQUENCE=AAGGACCTGGTGGGACAGAT PRIMER_LEFT_12=53,20 PRIMER_RIGHT_12=210,20 PRIMER_LEFT_12_TM=59.799 PRIMER_RIGHT_12_TM=60.770 PRIMER_LEFT_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_GC_PERCENT=55.000 PRIMER_LEFT_12_SELF_ANY=7.00 PRIMER_RIGHT_12_SELF_ANY=5.00 PRIMER_LEFT_12_SELF_END=5.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_END_STABILITY=7.9000 PRIMER_RIGHT_12_END_STABILITY=6.6000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PAIR_12_PRODUCT_SIZE=158 PRIMER_PAIR_13_PENALTY=1.019625 PRIMER_LEFT_13_PENALTY=0.249989 PRIMER_RIGHT_13_PENALTY=0.769636 PRIMER_LEFT_13_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_13_SEQUENCE=AAGGACCTGGTGGGACAGAT PRIMER_LEFT_13=83,20 PRIMER_RIGHT_13=210,20 PRIMER_LEFT_13_TM=59.750 PRIMER_RIGHT_13_TM=60.770 PRIMER_LEFT_13_GC_PERCENT=50.000 PRIMER_RIGHT_13_GC_PERCENT=55.000 PRIMER_LEFT_13_SELF_ANY=7.00 PRIMER_RIGHT_13_SELF_ANY=5.00 PRIMER_LEFT_13_SELF_END=1.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_END_STABILITY=8.5000 PRIMER_RIGHT_13_END_STABILITY=6.6000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=4.00 PRIMER_PAIR_13_PRODUCT_SIZE=128 PRIMER_PAIR_14_PENALTY=1.044583 PRIMER_LEFT_14_PENALTY=1.026872 PRIMER_RIGHT_14_PENALTY=0.017711 PRIMER_LEFT_14_SEQUENCE=CCACGGATTATCCTTCCATTT PRIMER_RIGHT_14_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_14=85,21 PRIMER_RIGHT_14=220,20 PRIMER_LEFT_14_TM=60.027 PRIMER_RIGHT_14_TM=59.982 PRIMER_LEFT_14_GC_PERCENT=42.857 PRIMER_RIGHT_14_GC_PERCENT=55.000 PRIMER_LEFT_14_SELF_ANY=7.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=4.00 PRIMER_LEFT_14_END_STABILITY=7.2000 PRIMER_RIGHT_14_END_STABILITY=9.7000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PAIR_14_PRODUCT_SIZE=136 PRIMER_PAIR_15_PENALTY=1.049246 PRIMER_LEFT_15_PENALTY=1.031536 PRIMER_RIGHT_15_PENALTY=0.017711 PRIMER_LEFT_15_SEQUENCE=GACCACGGATTATCCTTCCAT PRIMER_RIGHT_15_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_15=83,21 PRIMER_RIGHT_15=220,20 PRIMER_LEFT_15_TM=60.032 PRIMER_RIGHT_15_TM=59.982 PRIMER_LEFT_15_GC_PERCENT=47.619 PRIMER_RIGHT_15_GC_PERCENT=55.000 PRIMER_LEFT_15_SELF_ANY=7.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=4.00 PRIMER_LEFT_15_END_STABILITY=8.1000 PRIMER_RIGHT_15_END_STABILITY=9.7000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=2.00 PRIMER_PAIR_15_PRODUCT_SIZE=138 PRIMER_PAIR_16_PENALTY=1.116938 PRIMER_LEFT_16_PENALTY=1.099227 PRIMER_RIGHT_16_PENALTY=0.017711 PRIMER_LEFT_16_SEQUENCE=TCAGCTTTTTCCCCTTAGGA PRIMER_RIGHT_16_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_16=46,20 PRIMER_RIGHT_16=220,20 PRIMER_LEFT_16_TM=58.901 PRIMER_RIGHT_16_TM=59.982 PRIMER_LEFT_16_GC_PERCENT=45.000 PRIMER_RIGHT_16_GC_PERCENT=55.000 PRIMER_LEFT_16_SELF_ANY=5.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=4.00 PRIMER_LEFT_16_END_STABILITY=7.2000 PRIMER_RIGHT_16_END_STABILITY=9.7000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=0.00 PRIMER_PAIR_16_PRODUCT_SIZE=175 PRIMER_PAIR_17_PENALTY=1.153761 PRIMER_LEFT_17_PENALTY=1.136051 PRIMER_RIGHT_17_PENALTY=0.017711 PRIMER_LEFT_17_SEQUENCE=GGTATAGCCTTAGGACCACGG PRIMER_RIGHT_17_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_17=70,21 PRIMER_RIGHT_17=220,20 PRIMER_LEFT_17_TM=59.864 PRIMER_RIGHT_17_TM=59.982 PRIMER_LEFT_17_GC_PERCENT=57.143 PRIMER_RIGHT_17_GC_PERCENT=55.000 PRIMER_LEFT_17_SELF_ANY=5.00 PRIMER_RIGHT_17_SELF_ANY=6.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=4.00 PRIMER_LEFT_17_END_STABILITY=9.9000 PRIMER_RIGHT_17_END_STABILITY=9.7000 PRIMER_PAIR_17_COMPL_ANY=4.00 PRIMER_PAIR_17_COMPL_END=3.00 PRIMER_PAIR_17_PRODUCT_SIZE=151 PRIMER_PAIR_18_PENALTY=1.182933 PRIMER_LEFT_18_PENALTY=1.026872 PRIMER_RIGHT_18_PENALTY=0.156061 PRIMER_LEFT_18_SEQUENCE=CCACGGATTATCCTTCCATTT PRIMER_RIGHT_18_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_18=85,21 PRIMER_RIGHT_18=218,20 PRIMER_LEFT_18_TM=60.027 PRIMER_RIGHT_18_TM=59.844 PRIMER_LEFT_18_GC_PERCENT=42.857 PRIMER_RIGHT_18_GC_PERCENT=55.000 PRIMER_LEFT_18_SELF_ANY=7.00 PRIMER_RIGHT_18_SELF_ANY=5.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=3.00 PRIMER_LEFT_18_END_STABILITY=7.2000 PRIMER_RIGHT_18_END_STABILITY=8.2000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PAIR_18_PRODUCT_SIZE=134 PRIMER_PAIR_19_PENALTY=1.187596 PRIMER_LEFT_19_PENALTY=1.031536 PRIMER_RIGHT_19_PENALTY=0.156061 PRIMER_LEFT_19_SEQUENCE=GACCACGGATTATCCTTCCAT PRIMER_RIGHT_19_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_19=83,21 PRIMER_RIGHT_19=218,20 PRIMER_LEFT_19_TM=60.032 PRIMER_RIGHT_19_TM=59.844 PRIMER_LEFT_19_GC_PERCENT=47.619 PRIMER_RIGHT_19_GC_PERCENT=55.000 PRIMER_LEFT_19_SELF_ANY=7.00 PRIMER_RIGHT_19_SELF_ANY=5.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_END_STABILITY=8.1000 PRIMER_RIGHT_19_END_STABILITY=8.2000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=1.00 PRIMER_PAIR_19_PRODUCT_SIZE=136 PRIMER_PAIR_20_PENALTY=1.192524 PRIMER_LEFT_20_PENALTY=0.422888 PRIMER_RIGHT_20_PENALTY=0.769636 PRIMER_LEFT_20_SEQUENCE=GCTTTTTCCCCTTAGGACCA PRIMER_RIGHT_20_SEQUENCE=AAGGACCTGGTGGGACAGAT PRIMER_LEFT_20=49,20 PRIMER_RIGHT_20=210,20 PRIMER_LEFT_20_TM=60.423 PRIMER_RIGHT_20_TM=60.770 PRIMER_LEFT_20_GC_PERCENT=50.000 PRIMER_RIGHT_20_GC_PERCENT=55.000 PRIMER_LEFT_20_SELF_ANY=5.00 PRIMER_RIGHT_20_SELF_ANY=5.00 PRIMER_LEFT_20_SELF_END=1.00 PRIMER_RIGHT_20_SELF_END=2.00 PRIMER_LEFT_20_END_STABILITY=7.9000 PRIMER_RIGHT_20_END_STABILITY=6.6000 PRIMER_PAIR_20_COMPL_ANY=5.00 PRIMER_PAIR_20_COMPL_END=3.00 PRIMER_PAIR_20_PRODUCT_SIZE=162 PRIMER_PAIR_21_PENALTY=1.201715 PRIMER_LEFT_21_PENALTY=1.184005 PRIMER_RIGHT_21_PENALTY=0.017711 PRIMER_LEFT_21_SEQUENCE=TTTCCCCTTAGGACCAAGGTA PRIMER_RIGHT_21_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_21=53,21 PRIMER_RIGHT_21=220,20 PRIMER_LEFT_21_TM=59.816 PRIMER_RIGHT_21_TM=59.982 PRIMER_LEFT_21_GC_PERCENT=47.619 PRIMER_RIGHT_21_GC_PERCENT=55.000 PRIMER_LEFT_21_SELF_ANY=7.00 PRIMER_RIGHT_21_SELF_ANY=6.00 PRIMER_LEFT_21_SELF_END=3.00 PRIMER_RIGHT_21_SELF_END=4.00 PRIMER_LEFT_21_END_STABILITY=6.9000 PRIMER_RIGHT_21_END_STABILITY=9.7000 PRIMER_PAIR_21_COMPL_ANY=4.00 PRIMER_PAIR_21_COMPL_END=3.00 PRIMER_PAIR_21_PRODUCT_SIZE=168 PRIMER_PAIR_22_PENALTY=1.205374 PRIMER_LEFT_22_PENALTY=1.187663 PRIMER_RIGHT_22_PENALTY=0.017711 PRIMER_LEFT_22_SEQUENCE=GCCTTAGGACCACGGATTATC PRIMER_RIGHT_22_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_22=76,21 PRIMER_RIGHT_22=220,20 PRIMER_LEFT_22_TM=59.812 PRIMER_RIGHT_22_TM=59.982 PRIMER_LEFT_22_GC_PERCENT=52.381 PRIMER_RIGHT_22_GC_PERCENT=55.000 PRIMER_LEFT_22_SELF_ANY=5.00 PRIMER_RIGHT_22_SELF_ANY=6.00 PRIMER_LEFT_22_SELF_END=5.00 PRIMER_RIGHT_22_SELF_END=4.00 PRIMER_LEFT_22_END_STABILITY=5.9000 PRIMER_RIGHT_22_END_STABILITY=9.7000 PRIMER_PAIR_22_COMPL_ANY=4.00 PRIMER_PAIR_22_COMPL_END=1.00 PRIMER_PAIR_22_PRODUCT_SIZE=145 PRIMER_PAIR_23_PENALTY=1.255288 PRIMER_LEFT_23_PENALTY=1.099227 PRIMER_RIGHT_23_PENALTY=0.156061 PRIMER_LEFT_23_SEQUENCE=TCAGCTTTTTCCCCTTAGGA PRIMER_RIGHT_23_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_23=46,20 PRIMER_RIGHT_23=218,20 PRIMER_LEFT_23_TM=58.901 PRIMER_RIGHT_23_TM=59.844 PRIMER_LEFT_23_GC_PERCENT=45.000 PRIMER_RIGHT_23_GC_PERCENT=55.000 PRIMER_LEFT_23_SELF_ANY=5.00 PRIMER_RIGHT_23_SELF_ANY=5.00 PRIMER_LEFT_23_SELF_END=3.00 PRIMER_RIGHT_23_SELF_END=3.00 PRIMER_LEFT_23_END_STABILITY=7.2000 PRIMER_RIGHT_23_END_STABILITY=8.2000 PRIMER_PAIR_23_COMPL_ANY=4.00 PRIMER_PAIR_23_COMPL_END=0.00 PRIMER_PAIR_23_PRODUCT_SIZE=173 PRIMER_PAIR_24_PENALTY=1.255443 PRIMER_LEFT_24_PENALTY=0.201280 PRIMER_RIGHT_24_PENALTY=1.054163 PRIMER_LEFT_24_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_24_SEQUENCE=AAAGGACCTGGTGGGACAG PRIMER_LEFT_24=53,20 PRIMER_RIGHT_24=211,19 PRIMER_LEFT_24_TM=59.799 PRIMER_RIGHT_24_TM=59.946 PRIMER_LEFT_24_GC_PERCENT=50.000 PRIMER_RIGHT_24_GC_PERCENT=57.895 PRIMER_LEFT_24_SELF_ANY=7.00 PRIMER_RIGHT_24_SELF_ANY=5.00 PRIMER_LEFT_24_SELF_END=5.00 PRIMER_RIGHT_24_SELF_END=1.00 PRIMER_LEFT_24_END_STABILITY=7.9000 PRIMER_RIGHT_24_END_STABILITY=6.4000 PRIMER_PAIR_24_COMPL_ANY=5.00 PRIMER_PAIR_24_COMPL_END=3.00 PRIMER_PAIR_24_PRODUCT_SIZE=159 PRIMER_PAIR_25_PENALTY=1.292112 PRIMER_LEFT_25_PENALTY=1.136051 PRIMER_RIGHT_25_PENALTY=0.156061 PRIMER_LEFT_25_SEQUENCE=GGTATAGCCTTAGGACCACGG PRIMER_RIGHT_25_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_25=70,21 PRIMER_RIGHT_25=218,20 PRIMER_LEFT_25_TM=59.864 PRIMER_RIGHT_25_TM=59.844 PRIMER_LEFT_25_GC_PERCENT=57.143 PRIMER_RIGHT_25_GC_PERCENT=55.000 PRIMER_LEFT_25_SELF_ANY=5.00 PRIMER_RIGHT_25_SELF_ANY=5.00 PRIMER_LEFT_25_SELF_END=3.00 PRIMER_RIGHT_25_SELF_END=3.00 PRIMER_LEFT_25_END_STABILITY=9.9000 PRIMER_RIGHT_25_END_STABILITY=8.2000 PRIMER_PAIR_25_COMPL_ANY=4.00 PRIMER_PAIR_25_COMPL_END=3.00 PRIMER_PAIR_25_PRODUCT_SIZE=149 PRIMER_PAIR_26_PENALTY=1.295215 PRIMER_LEFT_26_PENALTY=0.525579 PRIMER_RIGHT_26_PENALTY=0.769636 PRIMER_LEFT_26_SEQUENCE=GGACCACGGATTATCCTTCC PRIMER_RIGHT_26_SEQUENCE=AAGGACCTGGTGGGACAGAT PRIMER_LEFT_26=82,20 PRIMER_RIGHT_26=210,20 PRIMER_LEFT_26_TM=60.526 PRIMER_RIGHT_26_TM=60.770 PRIMER_LEFT_26_GC_PERCENT=55.000 PRIMER_RIGHT_26_GC_PERCENT=55.000 PRIMER_LEFT_26_SELF_ANY=7.00 PRIMER_RIGHT_26_SELF_ANY=5.00 PRIMER_LEFT_26_SELF_END=3.00 PRIMER_RIGHT_26_SELF_END=2.00 PRIMER_LEFT_26_END_STABILITY=8.2000 PRIMER_RIGHT_26_END_STABILITY=6.6000 PRIMER_PAIR_26_COMPL_ANY=5.00 PRIMER_PAIR_26_COMPL_END=4.00 PRIMER_PAIR_26_PRODUCT_SIZE=129 PRIMER_PAIR_27_PENALTY=1.302983 PRIMER_LEFT_27_PENALTY=1.285273 PRIMER_RIGHT_27_PENALTY=0.017711 PRIMER_LEFT_27_SEQUENCE=CCACGGATTATCCTTCCATT PRIMER_RIGHT_27_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_27=85,20 PRIMER_RIGHT_27=220,20 PRIMER_LEFT_27_TM=58.715 PRIMER_RIGHT_27_TM=59.982 PRIMER_LEFT_27_GC_PERCENT=45.000 PRIMER_RIGHT_27_GC_PERCENT=55.000 PRIMER_LEFT_27_SELF_ANY=7.00 PRIMER_RIGHT_27_SELF_ANY=6.00 PRIMER_LEFT_27_SELF_END=1.00 PRIMER_RIGHT_27_SELF_END=4.00 PRIMER_LEFT_27_END_STABILITY=8.4000 PRIMER_RIGHT_27_END_STABILITY=9.7000 PRIMER_PAIR_27_COMPL_ANY=5.00 PRIMER_PAIR_27_COMPL_END=1.00 PRIMER_PAIR_27_PRODUCT_SIZE=136 PRIMER_PAIR_28_PENALTY=1.304152 PRIMER_LEFT_28_PENALTY=0.249989 PRIMER_RIGHT_28_PENALTY=1.054163 PRIMER_LEFT_28_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_28_SEQUENCE=AAAGGACCTGGTGGGACAG PRIMER_LEFT_28=83,20 PRIMER_RIGHT_28=211,19 PRIMER_LEFT_28_TM=59.750 PRIMER_RIGHT_28_TM=59.946 PRIMER_LEFT_28_GC_PERCENT=50.000 PRIMER_RIGHT_28_GC_PERCENT=57.895 PRIMER_LEFT_28_SELF_ANY=7.00 PRIMER_RIGHT_28_SELF_ANY=5.00 PRIMER_LEFT_28_SELF_END=1.00 PRIMER_RIGHT_28_SELF_END=1.00 PRIMER_LEFT_28_END_STABILITY=8.5000 PRIMER_RIGHT_28_END_STABILITY=6.4000 PRIMER_PAIR_28_COMPL_ANY=5.00 PRIMER_PAIR_28_COMPL_END=3.00 PRIMER_PAIR_28_PRODUCT_SIZE=129 PRIMER_PAIR_29_PENALTY=1.305530 PRIMER_LEFT_29_PENALTY=1.287819 PRIMER_RIGHT_29_PENALTY=0.017711 PRIMER_LEFT_29_SEQUENCE=AGCTTTTTCCCCTTAGGACC PRIMER_RIGHT_29_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_29=48,20 PRIMER_RIGHT_29=220,20 PRIMER_LEFT_29_TM=58.712 PRIMER_RIGHT_29_TM=59.982 PRIMER_LEFT_29_GC_PERCENT=50.000 PRIMER_RIGHT_29_GC_PERCENT=55.000 PRIMER_LEFT_29_SELF_ANY=5.00 PRIMER_RIGHT_29_SELF_ANY=6.00 PRIMER_LEFT_29_SELF_END=3.00 PRIMER_RIGHT_29_SELF_END=4.00 PRIMER_LEFT_29_END_STABILITY=9.1000 PRIMER_RIGHT_29_END_STABILITY=9.7000 PRIMER_PAIR_29_COMPL_ANY=4.00 PRIMER_PAIR_29_COMPL_END=2.00 PRIMER_PAIR_29_PRODUCT_SIZE=173 PRIMER_PAIR_30_PENALTY=1.305884 PRIMER_LEFT_30_PENALTY=1.288173 PRIMER_RIGHT_30_PENALTY=0.017711 PRIMER_LEFT_30_SEQUENCE=TCAGCTTTTTCCCCTTAGGAC PRIMER_RIGHT_30_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_30=46,21 PRIMER_RIGHT_30=220,20 PRIMER_LEFT_30_TM=59.712 PRIMER_RIGHT_30_TM=59.982 PRIMER_LEFT_30_GC_PERCENT=47.619 PRIMER_RIGHT_30_GC_PERCENT=55.000 PRIMER_LEFT_30_SELF_ANY=5.00 PRIMER_RIGHT_30_SELF_ANY=6.00 PRIMER_LEFT_30_SELF_END=1.00 PRIMER_RIGHT_30_SELF_END=4.00 PRIMER_LEFT_30_END_STABILITY=7.6000 PRIMER_RIGHT_30_END_STABILITY=9.7000 PRIMER_PAIR_30_COMPL_ANY=4.00 PRIMER_PAIR_30_COMPL_END=1.00 PRIMER_PAIR_30_PRODUCT_SIZE=175 PRIMER_PAIR_31_PENALTY=1.335744 PRIMER_LEFT_31_PENALTY=1.026872 PRIMER_RIGHT_31_PENALTY=0.308871 PRIMER_LEFT_31_SEQUENCE=CCACGGATTATCCTTCCATTT PRIMER_RIGHT_31_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_31=85,21 PRIMER_RIGHT_31=217,20 PRIMER_LEFT_31_TM=60.027 PRIMER_RIGHT_31_TM=59.691 PRIMER_LEFT_31_GC_PERCENT=42.857 PRIMER_RIGHT_31_GC_PERCENT=55.000 PRIMER_LEFT_31_SELF_ANY=7.00 PRIMER_RIGHT_31_SELF_ANY=5.00 PRIMER_LEFT_31_SELF_END=0.00 PRIMER_RIGHT_31_SELF_END=1.00 PRIMER_LEFT_31_END_STABILITY=7.2000 PRIMER_RIGHT_31_END_STABILITY=9.4000 PRIMER_PAIR_31_COMPL_ANY=5.00 PRIMER_PAIR_31_COMPL_END=1.00 PRIMER_PAIR_31_PRODUCT_SIZE=133 PRIMER_PAIR_32_PENALTY=1.339296 PRIMER_LEFT_32_PENALTY=1.183236 PRIMER_RIGHT_32_PENALTY=0.156061 PRIMER_LEFT_32_SEQUENCE=TTCAGCTTTTTCCCCTTAGGA PRIMER_RIGHT_32_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_32=45,21 PRIMER_RIGHT_32=218,20 PRIMER_LEFT_32_TM=60.183 PRIMER_RIGHT_32_TM=59.844 PRIMER_LEFT_32_GC_PERCENT=42.857 PRIMER_RIGHT_32_GC_PERCENT=55.000 PRIMER_LEFT_32_SELF_ANY=5.00 PRIMER_RIGHT_32_SELF_ANY=5.00 PRIMER_LEFT_32_SELF_END=3.00 PRIMER_RIGHT_32_SELF_END=3.00 PRIMER_LEFT_32_END_STABILITY=7.2000 PRIMER_RIGHT_32_END_STABILITY=8.2000 PRIMER_PAIR_32_COMPL_ANY=4.00 PRIMER_PAIR_32_COMPL_END=0.00 PRIMER_PAIR_32_PRODUCT_SIZE=174 PRIMER_PAIR_33_PENALTY=1.340065 PRIMER_LEFT_33_PENALTY=1.184005 PRIMER_RIGHT_33_PENALTY=0.156061 PRIMER_LEFT_33_SEQUENCE=TTTCCCCTTAGGACCAAGGTA PRIMER_RIGHT_33_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_33=53,21 PRIMER_RIGHT_33=218,20 PRIMER_LEFT_33_TM=59.816 PRIMER_RIGHT_33_TM=59.844 PRIMER_LEFT_33_GC_PERCENT=47.619 PRIMER_RIGHT_33_GC_PERCENT=55.000 PRIMER_LEFT_33_SELF_ANY=7.00 PRIMER_RIGHT_33_SELF_ANY=5.00 PRIMER_LEFT_33_SELF_END=3.00 PRIMER_RIGHT_33_SELF_END=3.00 PRIMER_LEFT_33_END_STABILITY=6.9000 PRIMER_RIGHT_33_END_STABILITY=8.2000 PRIMER_PAIR_33_COMPL_ANY=5.00 PRIMER_PAIR_33_COMPL_END=3.00 PRIMER_PAIR_33_PRODUCT_SIZE=166 PRIMER_PAIR_34_PENALTY=1.340407 PRIMER_LEFT_34_PENALTY=1.031536 PRIMER_RIGHT_34_PENALTY=0.308871 PRIMER_LEFT_34_SEQUENCE=GACCACGGATTATCCTTCCAT PRIMER_RIGHT_34_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_34=83,21 PRIMER_RIGHT_34=217,20 PRIMER_LEFT_34_TM=60.032 PRIMER_RIGHT_34_TM=59.691 PRIMER_LEFT_34_GC_PERCENT=47.619 PRIMER_RIGHT_34_GC_PERCENT=55.000 PRIMER_LEFT_34_SELF_ANY=7.00 PRIMER_RIGHT_34_SELF_ANY=5.00 PRIMER_LEFT_34_SELF_END=2.00 PRIMER_RIGHT_34_SELF_END=1.00 PRIMER_LEFT_34_END_STABILITY=8.1000 PRIMER_RIGHT_34_END_STABILITY=9.4000 PRIMER_PAIR_34_COMPL_ANY=5.00 PRIMER_PAIR_34_COMPL_END=2.00 PRIMER_PAIR_34_PRODUCT_SIZE=135 PRIMER_PAIR_35_PENALTY=1.343724 PRIMER_LEFT_35_PENALTY=1.187663 PRIMER_RIGHT_35_PENALTY=0.156061 PRIMER_LEFT_35_SEQUENCE=GCCTTAGGACCACGGATTATC PRIMER_RIGHT_35_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_35=76,21 PRIMER_RIGHT_35=218,20 PRIMER_LEFT_35_TM=59.812 PRIMER_RIGHT_35_TM=59.844 PRIMER_LEFT_35_GC_PERCENT=52.381 PRIMER_RIGHT_35_GC_PERCENT=55.000 PRIMER_LEFT_35_SELF_ANY=5.00 PRIMER_RIGHT_35_SELF_ANY=5.00 PRIMER_LEFT_35_SELF_END=5.00 PRIMER_RIGHT_35_SELF_END=3.00 PRIMER_LEFT_35_END_STABILITY=5.9000 PRIMER_RIGHT_35_END_STABILITY=8.2000 PRIMER_PAIR_35_COMPL_ANY=4.00 PRIMER_PAIR_35_COMPL_END=1.00 PRIMER_PAIR_35_PRODUCT_SIZE=143 PRIMER_PAIR_36_PENALTY=1.347129 PRIMER_LEFT_36_PENALTY=1.329419 PRIMER_RIGHT_36_PENALTY=0.017711 PRIMER_LEFT_36_SEQUENCE=AGCCTTAGGACCACGGATTA PRIMER_RIGHT_36_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_36=75,20 PRIMER_RIGHT_36=220,20 PRIMER_LEFT_36_TM=58.671 PRIMER_RIGHT_36_TM=59.982 PRIMER_LEFT_36_GC_PERCENT=50.000 PRIMER_RIGHT_36_GC_PERCENT=55.000 PRIMER_LEFT_36_SELF_ANY=5.00 PRIMER_RIGHT_36_SELF_ANY=6.00 PRIMER_LEFT_36_SELF_END=2.00 PRIMER_RIGHT_36_SELF_END=4.00 PRIMER_LEFT_36_END_STABILITY=5.9000 PRIMER_RIGHT_36_END_STABILITY=9.7000 PRIMER_PAIR_36_COMPL_ANY=4.00 PRIMER_PAIR_36_COMPL_END=0.00 PRIMER_PAIR_36_PRODUCT_SIZE=146 PRIMER_PAIR_37_PENALTY=1.347129 PRIMER_LEFT_37_PENALTY=1.329419 PRIMER_RIGHT_37_PENALTY=0.017711 PRIMER_LEFT_37_SEQUENCE=TAGCCTTAGGACCACGGATT PRIMER_RIGHT_37_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_37=74,20 PRIMER_RIGHT_37=220,20 PRIMER_LEFT_37_TM=58.671 PRIMER_RIGHT_37_TM=59.982 PRIMER_LEFT_37_GC_PERCENT=50.000 PRIMER_RIGHT_37_GC_PERCENT=55.000 PRIMER_LEFT_37_SELF_ANY=5.00 PRIMER_RIGHT_37_SELF_ANY=6.00 PRIMER_LEFT_37_SELF_END=1.00 PRIMER_RIGHT_37_SELF_END=4.00 PRIMER_LEFT_37_END_STABILITY=8.1000 PRIMER_RIGHT_37_END_STABILITY=9.7000 PRIMER_PAIR_37_COMPL_ANY=4.00 PRIMER_PAIR_37_COMPL_END=1.00 PRIMER_PAIR_37_PRODUCT_SIZE=147 PRIMER_PAIR_38_PENALTY=1.408099 PRIMER_LEFT_38_PENALTY=1.099227 PRIMER_RIGHT_38_PENALTY=0.308871 PRIMER_LEFT_38_SEQUENCE=TCAGCTTTTTCCCCTTAGGA PRIMER_RIGHT_38_SEQUENCE=CTCTTCAAAGGACCTGGTGG PRIMER_LEFT_38=46,20 PRIMER_RIGHT_38=217,20 PRIMER_LEFT_38_TM=58.901 PRIMER_RIGHT_38_TM=59.691 PRIMER_LEFT_38_GC_PERCENT=45.000 PRIMER_RIGHT_38_GC_PERCENT=55.000 PRIMER_LEFT_38_SELF_ANY=5.00 PRIMER_RIGHT_38_SELF_ANY=5.00 PRIMER_LEFT_38_SELF_END=3.00 PRIMER_RIGHT_38_SELF_END=1.00 PRIMER_LEFT_38_END_STABILITY=7.2000 PRIMER_RIGHT_38_END_STABILITY=9.4000 PRIMER_PAIR_38_COMPL_ANY=4.00 PRIMER_PAIR_38_COMPL_END=1.00 PRIMER_PAIR_38_PRODUCT_SIZE=172 PRIMER_PAIR_39_PENALTY=1.441333 PRIMER_LEFT_39_PENALTY=1.285273 PRIMER_RIGHT_39_PENALTY=0.156061 PRIMER_LEFT_39_SEQUENCE=CCACGGATTATCCTTCCATT PRIMER_RIGHT_39_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_39=85,20 PRIMER_RIGHT_39=218,20 PRIMER_LEFT_39_TM=58.715 PRIMER_RIGHT_39_TM=59.844 PRIMER_LEFT_39_GC_PERCENT=45.000 PRIMER_RIGHT_39_GC_PERCENT=55.000 PRIMER_LEFT_39_SELF_ANY=7.00 PRIMER_RIGHT_39_SELF_ANY=5.00 PRIMER_LEFT_39_SELF_END=1.00 PRIMER_RIGHT_39_SELF_END=3.00 PRIMER_LEFT_39_END_STABILITY=8.4000 PRIMER_RIGHT_39_END_STABILITY=8.2000 PRIMER_PAIR_39_COMPL_ANY=5.00 PRIMER_PAIR_39_COMPL_END=0.00 PRIMER_PAIR_39_PRODUCT_SIZE=134 = PRIMER_NUM_RETURN=8 SEQUENCE_ID=09.RHAP33FA9.seq SEQUENCE_TEMPLATE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG SEQUENCE_INCLUDED_REGION=0,371 SEQUENCE_TARGET=151,18 SEQUENCE_TARGET=192,53 SEQUENCE_TARGET=248,16 PRIMER_LEFT_NUM_RETURNED=8 PRIMER_RIGHT_NUM_RETURNED=8 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=8 PRIMER_PAIR_0_PENALTY=8.299756 PRIMER_LEFT_0_PENALTY=4.294975 PRIMER_RIGHT_0_PENALTY=4.004782 PRIMER_LEFT_0_SEQUENCE=TGAGACATCCTGTTACCATGTAGG PRIMER_RIGHT_0_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_0=19,24 PRIMER_RIGHT_0=191,18 PRIMER_LEFT_0_TM=60.295 PRIMER_RIGHT_0_TM=62.005 PRIMER_LEFT_0_GC_PERCENT=45.833 PRIMER_RIGHT_0_GC_PERCENT=72.222 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=1.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=173 PRIMER_PAIR_1_PENALTY=8.306042 PRIMER_LEFT_1_PENALTY=4.301260 PRIMER_RIGHT_1_PENALTY=4.004782 PRIMER_LEFT_1_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCCA PRIMER_RIGHT_1_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_1=67,24 PRIMER_RIGHT_1=191,18 PRIMER_LEFT_1_TM=60.301 PRIMER_RIGHT_1_TM=62.005 PRIMER_LEFT_1_GC_PERCENT=37.500 PRIMER_RIGHT_1_GC_PERCENT=72.222 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=125 PRIMER_PAIR_2_PENALTY=8.589410 PRIMER_LEFT_2_PENALTY=4.584629 PRIMER_RIGHT_2_PENALTY=4.004782 PRIMER_LEFT_2_SEQUENCE=GAGACATCCTGTTACCATGTAGGA PRIMER_RIGHT_2_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_2=20,24 PRIMER_RIGHT_2=191,18 PRIMER_LEFT_2_TM=59.415 PRIMER_RIGHT_2_TM=62.005 PRIMER_LEFT_2_GC_PERCENT=45.833 PRIMER_RIGHT_2_GC_PERCENT=72.222 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=172 PRIMER_PAIR_3_PENALTY=8.956691 PRIMER_LEFT_3_PENALTY=4.951910 PRIMER_RIGHT_3_PENALTY=4.004782 PRIMER_LEFT_3_SEQUENCE=TTGAGACATCCTGTTACCATGT PRIMER_RIGHT_3_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_3=18,22 PRIMER_RIGHT_3=191,18 PRIMER_LEFT_3_TM=57.048 PRIMER_RIGHT_3_TM=62.005 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=72.222 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=1.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=174 PRIMER_PAIR_4_PENALTY=9.135884 PRIMER_LEFT_4_PENALTY=5.131103 PRIMER_RIGHT_4_PENALTY=4.004782 PRIMER_LEFT_4_SEQUENCE=AGAGAGGTTTCATTTTCTCTGCT PRIMER_RIGHT_4_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_4=48,23 PRIMER_RIGHT_4=191,18 PRIMER_LEFT_4_TM=57.869 PRIMER_RIGHT_4_TM=62.005 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=72.222 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=144 PRIMER_PAIR_5_PENALTY=9.202456 PRIMER_LEFT_5_PENALTY=5.197674 PRIMER_RIGHT_5_PENALTY=4.004782 PRIMER_LEFT_5_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCC PRIMER_RIGHT_5_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_5=67,23 PRIMER_RIGHT_5=191,18 PRIMER_LEFT_5_TM=57.802 PRIMER_RIGHT_5_TM=62.005 PRIMER_LEFT_5_GC_PERCENT=39.130 PRIMER_RIGHT_5_GC_PERCENT=72.222 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=1.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_END_STABILITY=9.7000 PRIMER_RIGHT_5_END_STABILITY=9.4000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PAIR_5_PRODUCT_SIZE=125 PRIMER_PAIR_6_PENALTY=9.279205 PRIMER_LEFT_6_PENALTY=5.274423 PRIMER_RIGHT_6_PENALTY=4.004782 PRIMER_LEFT_6_SEQUENCE=GACATCCTGTTACCATGTAGGAGTT PRIMER_RIGHT_6_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_6=22,25 PRIMER_RIGHT_6=191,18 PRIMER_LEFT_6_TM=59.726 PRIMER_RIGHT_6_TM=62.005 PRIMER_LEFT_6_GC_PERCENT=44.000 PRIMER_RIGHT_6_GC_PERCENT=72.222 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=1.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_END_STABILITY=6.4000 PRIMER_RIGHT_6_END_STABILITY=9.4000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=2.00 PRIMER_PAIR_6_PRODUCT_SIZE=170 PRIMER_PAIR_7_PENALTY=9.284951 PRIMER_LEFT_7_PENALTY=5.280169 PRIMER_RIGHT_7_PENALTY=4.004782 PRIMER_LEFT_7_SEQUENCE=CTGCTAAGTAATGAGTTCAAAGCC PRIMER_RIGHT_7_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_7=66,24 PRIMER_RIGHT_7=191,18 PRIMER_LEFT_7_TM=58.720 PRIMER_RIGHT_7_TM=62.005 PRIMER_LEFT_7_GC_PERCENT=41.667 PRIMER_RIGHT_7_GC_PERCENT=72.222 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=1.00 PRIMER_LEFT_7_SELF_END=1.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=9.4000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PAIR_7_PRODUCT_SIZE=126 = SEQUENCE_ID=09.RHAP34FA9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT SEQUENCE_INCLUDED_REGION=20,280 SEQUENCE_TARGET=44,42 PRIMER_LEFT_NUM_RETURNED=8 PRIMER_RIGHT_NUM_RETURNED=8 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=8 PRIMER_PAIR_0_PENALTY=7.200927 PRIMER_LEFT_0_PENALTY=6.971746 PRIMER_RIGHT_0_PENALTY=0.229181 PRIMER_LEFT_0_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_0_SEQUENCE=TTCCTCACAGAGGGAACAGG PRIMER_LEFT_0=20,24 PRIMER_RIGHT_0=166,20 PRIMER_LEFT_0_TM=57.028 PRIMER_RIGHT_0_TM=60.229 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=5.8000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=147 PRIMER_PAIR_1_PENALTY=7.687872 PRIMER_LEFT_1_PENALTY=6.971746 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_LEFT_1_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_1_SEQUENCE=CAGGGGCTGTTTCTGATTCT PRIMER_LEFT_1=20,24 PRIMER_RIGHT_1=150,20 PRIMER_LEFT_1_TM=57.028 PRIMER_RIGHT_1_TM=59.284 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=5.8000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=131 PRIMER_PAIR_2_PENALTY=7.830958 PRIMER_LEFT_2_PENALTY=6.971746 PRIMER_RIGHT_2_PENALTY=0.859212 PRIMER_LEFT_2_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_2_SEQUENCE=ACAGGGGCTGTTTCTGATTC PRIMER_LEFT_2=20,24 PRIMER_RIGHT_2=151,20 PRIMER_LEFT_2_TM=57.028 PRIMER_RIGHT_2_TM=59.141 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=132 PRIMER_PAIR_3_PENALTY=7.830958 PRIMER_LEFT_3_PENALTY=6.971746 PRIMER_RIGHT_3_PENALTY=0.859212 PRIMER_LEFT_3_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_3_SEQUENCE=GAACAGGGGCTGTTTCTGAT PRIMER_LEFT_3=20,24 PRIMER_RIGHT_3=153,20 PRIMER_LEFT_3_TM=57.028 PRIMER_RIGHT_3_TM=59.141 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=5.8000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=134 PRIMER_PAIR_4_PENALTY=8.039099 PRIMER_LEFT_4_PENALTY=6.971746 PRIMER_RIGHT_4_PENALTY=1.067353 PRIMER_LEFT_4_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_4_SEQUENCE=GGGCTGTTTCTGATTCTTTCC PRIMER_LEFT_4=20,24 PRIMER_RIGHT_4=147,21 PRIMER_LEFT_4_TM=57.028 PRIMER_RIGHT_4_TM=60.067 PRIMER_LEFT_4_GC_PERCENT=33.333 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=128 PRIMER_PAIR_5_PENALTY=8.039099 PRIMER_LEFT_5_PENALTY=6.971746 PRIMER_RIGHT_5_PENALTY=1.067353 PRIMER_LEFT_5_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_5_SEQUENCE=GGGGCTGTTTCTGATTCTTTC PRIMER_LEFT_5=20,24 PRIMER_RIGHT_5=148,21 PRIMER_LEFT_5_TM=57.028 PRIMER_RIGHT_5_TM=60.067 PRIMER_LEFT_5_GC_PERCENT=33.333 PRIMER_RIGHT_5_GC_PERCENT=47.619 PRIMER_LEFT_5_SELF_ANY=6.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_LEFT_5_SELF_END=4.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_END_STABILITY=5.8000 PRIMER_RIGHT_5_END_STABILITY=7.0000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PAIR_5_PRODUCT_SIZE=129 PRIMER_PAIR_6_PENALTY=8.096344 PRIMER_LEFT_6_PENALTY=6.971746 PRIMER_RIGHT_6_PENALTY=1.124598 PRIMER_LEFT_6_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_6_SEQUENCE=ACAGGGGCTGTTTCTGATTCT PRIMER_LEFT_6=20,24 PRIMER_RIGHT_6=151,21 PRIMER_LEFT_6_TM=57.028 PRIMER_RIGHT_6_TM=60.125 PRIMER_LEFT_6_GC_PERCENT=33.333 PRIMER_RIGHT_6_GC_PERCENT=47.619 PRIMER_LEFT_6_SELF_ANY=6.00 PRIMER_RIGHT_6_SELF_ANY=5.00 PRIMER_LEFT_6_SELF_END=4.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_END_STABILITY=5.8000 PRIMER_RIGHT_6_END_STABILITY=6.6000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PAIR_6_PRODUCT_SIZE=132 PRIMER_PAIR_7_PENALTY=8.199338 PRIMER_LEFT_7_PENALTY=6.971746 PRIMER_RIGHT_7_PENALTY=1.227592 PRIMER_LEFT_7_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_7_SEQUENCE=TTTGTGTTGCTTGCATTTTTG PRIMER_LEFT_7=20,24 PRIMER_RIGHT_7=192,21 PRIMER_LEFT_7_TM=57.028 PRIMER_RIGHT_7_TM=59.772 PRIMER_LEFT_7_GC_PERCENT=33.333 PRIMER_RIGHT_7_GC_PERCENT=33.333 PRIMER_LEFT_7_SELF_ANY=6.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_LEFT_7_SELF_END=4.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_END_STABILITY=5.8000 PRIMER_RIGHT_7_END_STABILITY=7.6000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PAIR_7_PRODUCT_SIZE=173 = SEQUENCE_ID=10.RHAP34FA10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=12,236 SEQUENCE_TARGET=155,32 PRIMER_LEFT_NUM_RETURNED=8 PRIMER_RIGHT_NUM_RETURNED=8 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=8 PRIMER_PAIR_0_PENALTY=1.750634 PRIMER_LEFT_0_PENALTY=0.649665 PRIMER_RIGHT_0_PENALTY=1.100969 PRIMER_LEFT_0_SEQUENCE=CCTGCAATGGGATGAATACA PRIMER_RIGHT_0_SEQUENCE=CAAGGACAGAACTGAACTGGC PRIMER_LEFT_0=101,20 PRIMER_RIGHT_0=229,21 PRIMER_LEFT_0_TM=59.350 PRIMER_RIGHT_0_TM=59.899 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.6000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=129 PRIMER_PAIR_1_PENALTY=1.750634 PRIMER_LEFT_1_PENALTY=0.649665 PRIMER_RIGHT_1_PENALTY=1.100969 PRIMER_LEFT_1_SEQUENCE=CCTGCAATGGGATGAATACA PRIMER_RIGHT_1_SEQUENCE=GCAAGGACAGAACTGAACTGG PRIMER_LEFT_1=101,20 PRIMER_RIGHT_1=230,21 PRIMER_LEFT_1_TM=59.350 PRIMER_RIGHT_1_TM=59.899 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=5.6000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=130 PRIMER_PAIR_2_PENALTY=2.364169 PRIMER_LEFT_2_PENALTY=1.263200 PRIMER_RIGHT_2_PENALTY=1.100969 PRIMER_LEFT_2_SEQUENCE=GATATCCTGCAATGGGATGAA PRIMER_RIGHT_2_SEQUENCE=CAAGGACAGAACTGAACTGGC PRIMER_LEFT_2=96,21 PRIMER_RIGHT_2=229,21 PRIMER_LEFT_2_TM=59.737 PRIMER_RIGHT_2_TM=59.899 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=134 PRIMER_PAIR_3_PENALTY=2.364169 PRIMER_LEFT_3_PENALTY=1.263200 PRIMER_RIGHT_3_PENALTY=1.100969 PRIMER_LEFT_3_SEQUENCE=GATATCCTGCAATGGGATGAA PRIMER_RIGHT_3_SEQUENCE=GCAAGGACAGAACTGAACTGG PRIMER_LEFT_3=96,21 PRIMER_RIGHT_3=230,21 PRIMER_LEFT_3_TM=59.737 PRIMER_RIGHT_3_TM=59.899 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=135 PRIMER_PAIR_4_PENALTY=2.578428 PRIMER_LEFT_4_PENALTY=0.649665 PRIMER_RIGHT_4_PENALTY=1.928763 PRIMER_LEFT_4_SEQUENCE=CCTGCAATGGGATGAATACA PRIMER_RIGHT_4_SEQUENCE=TGCAAGGACAGAACTGAACTG PRIMER_LEFT_4=101,20 PRIMER_RIGHT_4=231,21 PRIMER_LEFT_4_TM=59.350 PRIMER_RIGHT_4_TM=59.071 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=5.6000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=131 PRIMER_PAIR_5_PENALTY=2.673857 PRIMER_LEFT_5_PENALTY=1.427458 PRIMER_RIGHT_5_PENALTY=1.246400 PRIMER_LEFT_5_SEQUENCE=TGGGGTTCAAAACAGAAAGAA PRIMER_RIGHT_5_SEQUENCE=GGCTCCTACAACCTGTCCTCT PRIMER_LEFT_5=37,21 PRIMER_RIGHT_5=211,21 PRIMER_LEFT_5_TM=59.573 PRIMER_RIGHT_5_TM=59.754 PRIMER_LEFT_5_GC_PERCENT=38.095 PRIMER_RIGHT_5_GC_PERCENT=57.143 PRIMER_LEFT_5_SELF_ANY=3.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_END_STABILITY=7.0000 PRIMER_RIGHT_5_END_STABILITY=7.9000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PAIR_5_PRODUCT_SIZE=175 PRIMER_PAIR_6_PENALTY=2.683392 PRIMER_LEFT_6_PENALTY=1.427458 PRIMER_RIGHT_6_PENALTY=1.255934 PRIMER_LEFT_6_SEQUENCE=TGGGGTTCAAAACAGAAAGAA PRIMER_RIGHT_6_SEQUENCE=GGCTCCTACAACCTGTCCTC PRIMER_LEFT_6=37,21 PRIMER_RIGHT_6=211,20 PRIMER_LEFT_6_TM=59.573 PRIMER_RIGHT_6_TM=58.744 PRIMER_LEFT_6_GC_PERCENT=38.095 PRIMER_RIGHT_6_GC_PERCENT=60.000 PRIMER_LEFT_6_SELF_ANY=3.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_END_STABILITY=7.0000 PRIMER_RIGHT_6_END_STABILITY=7.9000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PAIR_6_PRODUCT_SIZE=175 PRIMER_PAIR_7_PENALTY=2.727005 PRIMER_LEFT_7_PENALTY=0.649665 PRIMER_RIGHT_7_PENALTY=2.077340 PRIMER_LEFT_7_SEQUENCE=CCTGCAATGGGATGAATACA PRIMER_RIGHT_7_SEQUENCE=AAGGACAGAACTGAACTGGCTC PRIMER_LEFT_7=101,20 PRIMER_RIGHT_7=228,22 PRIMER_LEFT_7_TM=59.350 PRIMER_RIGHT_7_TM=59.923 PRIMER_LEFT_7_GC_PERCENT=45.000 PRIMER_RIGHT_7_GC_PERCENT=50.000 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_LEFT_7_SELF_END=4.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_END_STABILITY=5.6000 PRIMER_RIGHT_7_END_STABILITY=9.4000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PAIR_7_PRODUCT_SIZE=128 = SEQUENCE_ID=13.RHAP34FB1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,244 SEQUENCE_TARGET=22,16 SEQUENCE_TARGET=57,34 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=14.RHAP34FB2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC SEQUENCE_INCLUDED_REGION=13,303 SEQUENCE_TARGET=177,33 SEQUENCE_TARGET=254,14 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = primer3-2.3.6/test/primer_high_tm_load_set.set0000664000077300007730000000023012231156245020414 0ustar ioanaioanaPrimer3 File - http://primer3.sourceforge.net P3_FILE_TYPE=settings P3_FILE_ID=Test with higher Tm PRIMER_MIN_TM=63 PRIMER_OPT_TM=65 PRIMER_MAX_TM=67 =primer3-2.3.6/test/primer_boundary1_output0000664000077300007730000032373212231156245017674 0ustar ioanaioanaPRIMER_FIRST_BASE_INDEX=1 SEQUENCE_ID=boundary_test_00 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-100 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_02 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_03 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="56-100" P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_04 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="50-54" P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, unacceptable product size 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_05 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MIN_SIZE=24 PRIMER_MAX_SIZE=24 PRIMER_OPT_SIZE=24 PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 3, ok 2 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 3, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=0.330996 PRIMER_LEFT_0_PENALTY=0.243965 PRIMER_RIGHT_0_PENALTY=0.087031 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATT PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAG PRIMER_LEFT_0=3,24 PRIMER_RIGHT_0=57,24 PRIMER_LEFT_0_TM=60.244 PRIMER_RIGHT_0_TM=59.913 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_06 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MIN_SIZE=26 PRIMER_MAX_SIZE=26 PRIMER_OPT_SIZE=26 P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 5, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_07 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_08 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="50-100" PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 SEQUENCE_INCLUDED_REGION=4,55 P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 6, ok 0 PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_09 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="50-100" SEQUENCE_INCLUDED_REGION=3,54 P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 5, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_10 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3 PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=1.826922 PRIMER_LEFT_0_PENALTY=1.177322 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTNATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=61.177 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=9.50 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.75 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 5, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 2, ok 3 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_12 PRIMER_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, ok 5 PRIMER_PAIR_EXPLAIN=considered 5, unacceptable product size 4, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.870774 PRIMER_LEFT_0_PENALTY=3.221174 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCNNATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=63.221 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=34.783 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=11.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_13 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.811551 PRIMER_LEFT_0_PENALTY=4.161950 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=AAAAAAAAAAAAAAAAAAAAAAAAA PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=55.838 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=0.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_14 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=.0001 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_15 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=20 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=31.102361 PRIMER_LEFT_0_PENALTY=30.452761 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=GGGGGGGGGGGGGGGGGGGGGGGGG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=90.453 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=100.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=0.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=1.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_16 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=.0001 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, GC content failed 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_17 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_18 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=4,55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 1, too many Ns 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 1, too many Ns 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_19 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,54 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_ERROR=SEQUENCE_INCLUDED_REGION length < min PRIMER_PRODUCT_SIZE_RANGE = SEQUENCE_ID=boundary_test_20 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE="54-55" SEQUENCE_INCLUDED_REGION=3,54 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, too many Ns 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_21 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_ID=boundary_test_22 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,6 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 2, ok 0 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_23 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,6 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 2, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_24 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=29,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 2, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,1 SEQUENCE_TARGET=29,4 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_26 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,1 SEQUENCE_TARGET=28,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, overlap target 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_27 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,1 SEQUENCE_TARGET=29,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap target 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_28 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=29,1 SEQUENCE_TARGET=30,4 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap target 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_29 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_30 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=29,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 4, too many Ns 3, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_31 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=1,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_32 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_33 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,6 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_34 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,6 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_35 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_36 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 SEQUENCE_EXCLUDED_REGION=29,4 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_37 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,1 SEQUENCE_EXCLUDED_REGION=28,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_37 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 SEQUENCE_EXCLUDED_REGION=29,5 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_38 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 SEQUENCE_EXCLUDED_REGION=30,4 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_39 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_40 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_41 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=11,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_42 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_43 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=9.99 PRIMER_PAIR_MAX_COMPL_END=9.99 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because one primer has end compl 10.00)" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, high end compl 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_44 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=10.01 PRIMER_PAIR_MAX_COMPL_END=10.01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_45 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=12.01 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=12.01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_46 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=11.99 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_PAIR_MAX_COMPL_ANY=11.99 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because self any for one primer is 12.00" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, high any compl 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_47 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_48 PRIMER_GC_CLAMP=1 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because of GC clamp)" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, GC clamp failed 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_49 PRIMER_GC_CLAMP=1 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=5.752125 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=3.020452 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=63.020 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_50 PRIMER_GC_CLAMP=2 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because of GC clamp)" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, GC clamp failed 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_51 PRIMER_PAIR_MAX_DIFF_TM=100 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=5.752125 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=3.020452 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=63.020 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_52 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=5.752125 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=3.020452 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=63.020 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_53 PRIMER_PAIR_MAX_DIFF_TM=0.28 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (due to max diff tm)" PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, tm diff too large 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_54 PRIMER_LIBERAL_BASE=1 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" PRIMER_WARNING=Unrecognized base in input sequence PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=5.752125 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=3.020452 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGG PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=63.020 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_55 PRIMER_LIBERAL_BASE=0 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=29,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should generate error" PRIMER_ERROR=Unrecognized base in input sequence = SEQUENCE_ID=poly_x_1 SEQUENCE_TEMPLATE=AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTTCCCCCC PRIMER_MAX_POLY_X=5 PRIMER_INTERNAL_MAX_POLY_X=6 PRIMER_PRODUCT_SIZE_RANGE=50-300 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PAIR_MAX_DIFF_TM=100 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_LEFT_EXPLAIN=considered 138, long poly-x seq 138, ok 0 PRIMER_RIGHT_EXPLAIN=considered 138, long poly-x seq 138, ok 0 PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=poly_x_2 PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 SEQUENCE_TEMPLATE=AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG PRIMER_LEFT_EXPLAIN=considered 131, ok 131 PRIMER_RIGHT_EXPLAIN=considered 131, ok 131 PRIMER_INTERNAL_EXPLAIN=considered 1585, ok 1585 PRIMER_PAIR_EXPLAIN=considered 64, unacceptable product size 48, ok 16 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=15.547212 PRIMER_LEFT_0_PENALTY=7.773606 PRIMER_RIGHT_0_PENALTY=7.773606 PRIMER_INTERNAL_0_PENALTY=1.499588 PRIMER_LEFT_0_SEQUENCE=CTTTTTGGGGGAAAAATTTTTCCCC PRIMER_RIGHT_0_SEQUENCE=GAAAAATTTTTCCCCCAAAAAGGGG PRIMER_INTERNAL_0_SEQUENCE=CCCTTTTTGGGGGAAAAAC PRIMER_LEFT_0=30,25 PRIMER_RIGHT_0=131,25 PRIMER_INTERNAL_0=88,19 PRIMER_LEFT_0_TM=67.774 PRIMER_RIGHT_0_TM=67.774 PRIMER_INTERNAL_0_TM=60.500 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_INTERNAL_0_GC_PERCENT=47.368 PRIMER_LEFT_0_SELF_ANY=18.00 PRIMER_RIGHT_0_SELF_ANY=12.00 PRIMER_INTERNAL_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=18.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_INTERNAL_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=10.9000 PRIMER_PAIR_0_COMPL_ANY=22.00 PRIMER_PAIR_0_COMPL_END=14.00 PRIMER_PAIR_0_PRODUCT_SIZE=102 PRIMER_PAIR_1_PENALTY=15.547212 PRIMER_LEFT_1_PENALTY=7.773606 PRIMER_RIGHT_1_PENALTY=7.773606 PRIMER_INTERNAL_1_PENALTY=1.499588 PRIMER_LEFT_1_SEQUENCE=CTTTTTGGGGGAAAAATTTTTCCCC PRIMER_RIGHT_1_SEQUENCE=GGAAAAATTTTTCCCCCAAAAAGGG PRIMER_INTERNAL_1_SEQUENCE=CCTTTTTGGGGGAAAAACC PRIMER_LEFT_1=30,25 PRIMER_RIGHT_1=132,25 PRIMER_INTERNAL_1=89,19 PRIMER_LEFT_1_TM=67.774 PRIMER_RIGHT_1_TM=67.774 PRIMER_INTERNAL_1_TM=60.500 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_INTERNAL_1_GC_PERCENT=47.368 PRIMER_LEFT_1_SELF_ANY=18.00 PRIMER_RIGHT_1_SELF_ANY=14.00 PRIMER_INTERNAL_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=18.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=10.9000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=23.00 PRIMER_PAIR_1_COMPL_END=19.00 PRIMER_PAIR_1_PRODUCT_SIZE=103 PRIMER_PAIR_2_PENALTY=15.547212 PRIMER_LEFT_2_PENALTY=7.773606 PRIMER_RIGHT_2_PENALTY=7.773606 PRIMER_INTERNAL_2_PENALTY=1.499588 PRIMER_LEFT_2_SEQUENCE=CTTTTTGGGGGAAAAATTTTTCCCC PRIMER_RIGHT_2_SEQUENCE=GGGAAAAATTTTTCCCCCAAAAAGG PRIMER_INTERNAL_2_SEQUENCE=CTTTTTGGGGGAAAAACCC PRIMER_LEFT_2=30,25 PRIMER_RIGHT_2=133,25 PRIMER_INTERNAL_2=90,19 PRIMER_LEFT_2_TM=67.774 PRIMER_RIGHT_2_TM=67.774 PRIMER_INTERNAL_2_TM=60.500 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_INTERNAL_2_GC_PERCENT=47.368 PRIMER_LEFT_2_SELF_ANY=18.00 PRIMER_RIGHT_2_SELF_ANY=16.00 PRIMER_INTERNAL_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=18.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_INTERNAL_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=10.9000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=24.00 PRIMER_PAIR_2_COMPL_END=22.00 PRIMER_PAIR_2_PRODUCT_SIZE=104 PRIMER_PAIR_3_PENALTY=15.547212 PRIMER_LEFT_3_PENALTY=7.773606 PRIMER_RIGHT_3_PENALTY=7.773606 PRIMER_INTERNAL_3_PENALTY=1.499588 PRIMER_LEFT_3_SEQUENCE=CTTTTTGGGGGAAAAATTTTTCCCC PRIMER_RIGHT_3_SEQUENCE=GGGGAAAAATTTTTCCCCCAAAAAG PRIMER_INTERNAL_3_SEQUENCE=CTTTTTGGGGGAAAAACCC PRIMER_LEFT_3=30,25 PRIMER_RIGHT_3=134,25 PRIMER_INTERNAL_3=90,19 PRIMER_LEFT_3_TM=67.774 PRIMER_RIGHT_3_TM=67.774 PRIMER_INTERNAL_3_TM=60.500 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_INTERNAL_3_GC_PERCENT=47.368 PRIMER_LEFT_3_SELF_ANY=18.00 PRIMER_RIGHT_3_SELF_ANY=18.00 PRIMER_INTERNAL_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=18.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=25.00 PRIMER_PAIR_3_COMPL_END=25.00 PRIMER_PAIR_3_PRODUCT_SIZE=105 PRIMER_PAIR_4_PENALTY=15.547212 PRIMER_LEFT_4_PENALTY=7.773606 PRIMER_RIGHT_4_PENALTY=7.773606 PRIMER_INTERNAL_4_PENALTY=1.499588 PRIMER_LEFT_4_SEQUENCE=CCTTTTTGGGGGAAAAATTTTTCCC PRIMER_RIGHT_4_SEQUENCE=GAAAAATTTTTCCCCCAAAAAGGGG PRIMER_INTERNAL_4_SEQUENCE=CCCTTTTTGGGGGAAAAAC PRIMER_LEFT_4=29,25 PRIMER_RIGHT_4=131,25 PRIMER_INTERNAL_4=88,19 PRIMER_LEFT_4_TM=67.774 PRIMER_RIGHT_4_TM=67.774 PRIMER_INTERNAL_4_TM=60.500 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_INTERNAL_4_GC_PERCENT=47.368 PRIMER_LEFT_4_SELF_ANY=16.00 PRIMER_RIGHT_4_SELF_ANY=12.00 PRIMER_INTERNAL_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=16.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=10.9000 PRIMER_PAIR_4_COMPL_ANY=23.00 PRIMER_PAIR_4_COMPL_END=19.00 PRIMER_PAIR_4_PRODUCT_SIZE=103 = SEQUENCE_ID=poly_x_3 SEQUENCE_TEMPLATE=AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTTGGGGGGGGGGGGGG PRIMER_MAX_POLY_X=6 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_LEFT_EXPLAIN=considered 155, long poly-x seq 57, ok 98 PRIMER_RIGHT_EXPLAIN=considered 155, long poly-x seq 72, ok 83 PRIMER_INTERNAL_EXPLAIN=considered 187, long poly-x seq 187, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_59 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=ok (excluded region is between G and N and end of left primer). PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_60 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (excluded region is between T and G at end of left primer). PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, overlap excluded region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_61 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,-1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (negative excluded region size). PRIMER_ERROR=Negative EXCLUDED_REGION length = SEQUENCE_ID=boundary_test_62 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=2,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED REGION PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_63 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=3,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers PRIMER_LEFT_EXPLAIN=considered 1, overlap excluded region 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_64 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=60,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with EXCLUDED_REGION outside of sequence PRIMER_ERROR=EXCLUDED_REGION beyond end of sequence = SEQUENCE_ID=boundary_test_65 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=59,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_66 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=57,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_67 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_EXCLUDED_REGION=57,2 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to pick primers and warn EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_WARNING=EXCLUDED_REGION outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1 PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, overlap excluded region 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_68 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,0 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=ok (TARGET is between G and N and end of left primer). PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_69 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (TARGET is between T and G at end of left primer). PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 2, ok 0 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_70 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,-1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (negative TARGET size). PRIMER_ERROR=Negative TARGET length = SEQUENCE_ID=boundary_test_71 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=2,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to find legal primers, and warn TARGET outside of INCLUDED REGION PRIMER_WARNING=TARGET outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_72 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=3,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers PRIMER_LEFT_EXPLAIN=considered 1, overlap target 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_73 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=60,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with TARGET outside of sequence PRIMER_ERROR=TARGET beyond end of sequence = SEQUENCE_ID=boundary_test_74 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=59,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to find primers (none spans target), and warn TARGET outside of INCLUDED_REGION PRIMER_WARNING=TARGET outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_75 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=57,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 0, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_76 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=57,2 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to pick primers, and warn TARGET outside of INCLUDED_REGION PRIMER_WARNING=TARGET outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1 PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, overlap target 1, ok 0 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_77 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=28,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (target is at TTG^NNN) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_78 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=28,5 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (target is the middle run of N) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_79 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=28,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 6, too many Ns 5, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_80 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=32,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_81 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=33,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 6, too many Ns 5, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_82 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=1,59 SEQUENCE_TARGET=33,0 1,1 59,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_83 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=1,59 SEQUENCE_TARGET=1,1 59,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (no target) PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no target 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_84 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=1,59 SEQUENCE_TARGET=1,1 33,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 8, too many Ns 7, ok 1 PRIMER_RIGHT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_85 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=1,59 SEQUENCE_TARGET=33,0 59,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed PRIMER_LEFT_EXPLAIN=considered 10, too many Ns 9, ok 1 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_86 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=35 PRIMER_MAX_SIZE=35 PRIMER_OPT_SIZE=35 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=75-75 SEQUENCE_TEMPLATE=NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (testing hard limit MAX_PRIMER_LENGTH) PRIMER_LEFT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_RIGHT_EXPLAIN=considered 5, too many Ns 4, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.202608 PRIMER_LEFT_0_PENALTY=2.838677 PRIMER_RIGHT_0_PENALTY=1.363932 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTATATATATATCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTATATATATATGAGT PRIMER_LEFT_0=3,35 PRIMER_RIGHT_0=77,35 PRIMER_LEFT_0_TM=62.839 PRIMER_RIGHT_0_TM=61.364 PRIMER_LEFT_0_GC_PERCENT=25.714 PRIMER_RIGHT_0_GC_PERCENT=25.714 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=8.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=13.00 PRIMER_PAIR_0_COMPL_END=8.00 PRIMER_PAIR_0_PRODUCT_SIZE=75 = SEQUENCE_ID=boundary_test_71 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TARGET=2,1 28,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should warn TARGET outside of INCLUDED REGION PRIMER_WARNING=TARGET outside of INCLUDED_REGION PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_72 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,56 PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succed with exactly one pair PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 2, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_73 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,56 PRIMER_PRODUCT_SIZE_RANGE=54-54 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (no primers with acceptable product size) PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 2, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_74 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,56 PRIMER_PRODUCT_SIZE_RANGE=54-55 55-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair PRIMER_LEFT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_RIGHT_EXPLAIN=considered 3, too many Ns 2, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_75 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_76 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,56 PRIMER_PRODUCT_SIZE_RANGE=55-55 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair PRIMER_LEFT_EXPLAIN=considered 2, too many Ns 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 2, too many Ns 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 2, unacceptable product size 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_77 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,82 PRIMER_PRODUCT_SIZE_RANGE=55-55 82-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product second PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1 PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 2, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 PRIMER_PAIR_1_PENALTY=3.381274 PRIMER_LEFT_1_PENALTY=2.731673 PRIMER_RIGHT_1_PENALTY=0.649600 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_1=3,25 PRIMER_RIGHT_1=84,25 PRIMER_LEFT_1_TM=62.732 PRIMER_RIGHT_1_TM=60.650 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 = SEQUENCE_ID=boundary_test_78 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,82 PRIMER_PRODUCT_SIZE_RANGE=82-82 55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product first PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1 PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2 PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 2, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=84,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=3.381274 PRIMER_LEFT_1_PENALTY=2.731673 PRIMER_RIGHT_1_PENALTY=0.649600 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_1=3,25 PRIMER_RIGHT_1=57,25 PRIMER_LEFT_1_TM=62.732 PRIMER_RIGHT_1_TM=60.650 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_79 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,82 PRIMER_PRODUCT_SIZE_RANGE=55-55 55-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product second PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1 PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2 PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 1, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 PRIMER_PAIR_1_PENALTY=3.381274 PRIMER_LEFT_1_PENALTY=2.731673 PRIMER_RIGHT_1_PENALTY=0.649600 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_1=3,25 PRIMER_RIGHT_1=84,25 PRIMER_LEFT_1_TM=62.732 PRIMER_RIGHT_1_TM=60.650 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=82 = SEQUENCE_ID=boundary_test_80 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,82 PRIMER_PRODUCT_SIZE_RANGE=82-82 55-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product first PRIMER_LEFT_EXPLAIN=considered 28, too many Ns 27, ok 1 PRIMER_RIGHT_EXPLAIN=considered 28, too many Ns 26, ok 2 PRIMER_PAIR_EXPLAIN=considered 3, unacceptable product size 1, ok 2 PRIMER_LEFT_NUM_RETURNED=2 PRIMER_RIGHT_NUM_RETURNED=2 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=2 PRIMER_PAIR_0_PENALTY=3.381274 PRIMER_LEFT_0_PENALTY=2.731673 PRIMER_RIGHT_0_PENALTY=0.649600 PRIMER_LEFT_0_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=84,25 PRIMER_LEFT_0_TM=62.732 PRIMER_RIGHT_0_TM=60.650 PRIMER_LEFT_0_GC_PERCENT=36.000 PRIMER_RIGHT_0_GC_PERCENT=36.000 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=10.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=82 PRIMER_PAIR_1_PENALTY=3.381274 PRIMER_LEFT_1_PENALTY=2.731673 PRIMER_RIGHT_1_PENALTY=0.649600 PRIMER_LEFT_1_SEQUENCE=CACGTCATCTCATCAAATTTGATTG PRIMER_RIGHT_1_SEQUENCE=GAATTGTTTGGAAATGACAGTGAGT PRIMER_LEFT_1=3,25 PRIMER_RIGHT_1=57,25 PRIMER_LEFT_1_TM=62.732 PRIMER_RIGHT_1_TM=60.650 PRIMER_LEFT_1_GC_PERCENT=36.000 PRIMER_RIGHT_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=12.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=10.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_81 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_PAIR_MAX_COMPL_ANY=11 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing pairwise complementarity checking) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.137556 PRIMER_LEFT_0_PENALTY=2.963671 PRIMER_RIGHT_0_PENALTY=1.173886 PRIMER_LEFT_0_SEQUENCE=AATGTGATTGCATCAAATGTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTGGAAATGACAATCACATT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.964 PRIMER_RIGHT_0_TM=61.174 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=11.00 PRIMER_PAIR_0_COMPL_END=10.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_82 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=10.99 PRIMER_PAIR_MAX_COMPL_ANY=10.99 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (self any for pairs) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, high any compl 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_83 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing pairwise complementarity checking) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.137556 PRIMER_LEFT_0_PENALTY=2.963671 PRIMER_RIGHT_0_PENALTY=1.173886 PRIMER_LEFT_0_SEQUENCE=AATGTGATTGCATCAAATGTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTGGAAATGACAATCACATT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.964 PRIMER_RIGHT_0_TM=61.174 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=11.00 PRIMER_PAIR_0_COMPL_END=10.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_84 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=9.99 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=9.99 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (self end for pairs) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, high end compl 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=boundary_test_85 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of product size ranges) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.137556 PRIMER_LEFT_0_PENALTY=2.963671 PRIMER_RIGHT_0_PENALTY=1.173886 PRIMER_LEFT_0_SEQUENCE=AATGTGATTGCATCAAATGTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTGGAAATGACAATCACATT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.964 PRIMER_RIGHT_0_TM=61.174 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=11.00 PRIMER_PAIR_0_COMPL_END=10.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_86 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TARGET=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of targets) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.137556 PRIMER_LEFT_0_PENALTY=2.963671 PRIMER_RIGHT_0_PENALTY=1.173886 PRIMER_LEFT_0_SEQUENCE=AATGTGATTGCATCAAATGTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTGGAAATGACAATCACATT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.964 PRIMER_RIGHT_0_TM=61.174 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=11.00 PRIMER_PAIR_0_COMPL_END=10.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_87 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TARGET=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (testing number of targets) PRIMER_ERROR=Too many elements for tag SEQUENCE_TARGET = SEQUENCE_ID=boundary_test_88 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_EXCLUDED_REGION=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of excluded regions) PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=4.137556 PRIMER_LEFT_0_PENALTY=2.963671 PRIMER_RIGHT_0_PENALTY=1.173886 PRIMER_LEFT_0_SEQUENCE=AATGTGATTGCATCAAATGTGATTG PRIMER_RIGHT_0_SEQUENCE=GAATTGTGGAAATGACAATCACATT PRIMER_LEFT_0=3,25 PRIMER_RIGHT_0=57,25 PRIMER_LEFT_0_TM=62.964 PRIMER_RIGHT_0_TM=61.174 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=11.00 PRIMER_PAIR_0_COMPL_END=10.00 PRIMER_PAIR_0_PRODUCT_SIZE=55 = SEQUENCE_ID=boundary_test_89 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_EXCLUDED_REGION=28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 28,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (testing number of excluded regions) PRIMER_ERROR=Too many elements for tag SEQUENCE_EXCLUDED_REGION = primer3-2.3.6/test/primer3web_v4_0_0_default_settings_output0000664000077300007730000002356212231156245023162 0ustar ioanaioanaP3_SETTINGS_FILE_USED=../primer3web_v4_0_0_default_settings.txt P3_FILE_TYPE=settings PRIMER_FIRST_BASE_INDEX=1 PRIMER_TASK=generic P3_FILE_ID=Settings for PCR amplification followed by Sanger sequencing on both strands using PCR primers PRIMER_MIN_THREE_PRIME_DISTANCE=3 PRIMER_EXPLAIN_FLAG=1 PRIMER_MISPRIMING_LIBRARY= PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=20.00 PRIMER_PRODUCT_SIZE_RANGE=150-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000 PRIMER_NUM_RETURN=5 PRIMER_MAX_END_STABILITY=9.0 PRIMER_MAX_SELF_ANY_TH=45.00 PRIMER_MAX_SELF_END_TH=35.00 PRIMER_PAIR_MAX_COMPL_ANY_TH=45.00 PRIMER_PAIR_MAX_COMPL_END_TH=35.00 PRIMER_MAX_HAIRPIN_TH=24.00 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=40.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=70.00 PRIMER_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_MAX_SIZE=23 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=59.0 PRIMER_MAX_TM=62.0 PRIMER_PAIR_MAX_DIFF_TM=5.0 PRIMER_TM_FORMULA=1 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_DIVALENT=1.5 PRIMER_DNTP_CONC=0.6 PRIMER_DNA_CONC=50.0 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0 PRIMER_LOWERCASE_MASKING=0 PRIMER_MIN_GC=30.0 PRIMER_MAX_GC=70.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_POLY_X=4 PRIMER_OUTSIDE_PENALTY=0 PRIMER_GC_CLAMP=0 PRIMER_LIBERAL_BASE=1 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_PICK_ANYWAY=1 PRIMER_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_WT_SELF_END_TH=0.0 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_WT_NUM_NS=0.0 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_WT_END_QUAL=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_END_TH=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_END_QUAL=0.0 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_PRODUCT_MAX_TM=1000000.0 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_SEQUENCING_ACCURACY=20 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MAX_SELF_END=3.00 PRIMER_QUALITY_RANGE_MAX=100 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_SEQUENCING_INTERVAL=250 PRIMER_INTERNAL_SALT_DIVALENT=1.5 PRIMER_MAX_SELF_ANY=8.00 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_SEQUENCING_SPACING=500 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_MIN_END_QUALITY=0 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_SEQUENCING_LEAD=50 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_MAX_END_GC=5 PRIMER_MIN_QUALITY=0 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_WT_SELF_ANY=0.0 PRIMER_MAX_TEMPLATE_MISPRIMING=12.00 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_SELF_END=0.0 PRIMER_PRODUCT_OPT_SIZE=0 PRIMER_PRODUCT_OPT_TM=0.0 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=24.00 PRIMER_INSIDE_PENALTY=-1.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_PRODUCT_MIN_TM=-1000000.0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_WT_SELF_END=0.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_TM_LT=1.0 P3_SETTINGS_FILE_END= SEQUENCE_ID=test_settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LEFT_EXPLAIN=considered 7867, GC content failed 203, low tm 3099, high tm 1432, high hairpin stability 4, long poly-x seq 168, ok 2961 PRIMER_RIGHT_EXPLAIN=considered 7867, GC content failed 226, low tm 3298, high tm 1334, high hairpin stability 1, long poly-x seq 168, ok 2840 PRIMER_PAIR_EXPLAIN=considered 266, unacceptable product size 260, primer in pair overlaps a primer in a better pair 25, ok 6 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.046400 PRIMER_LEFT_0_PENALTY=0.005753 PRIMER_RIGHT_0_PENALTY=0.040646 PRIMER_LEFT_0_SEQUENCE=tgccatcaatgacctccact PRIMER_RIGHT_0_SEQUENCE=tgagctgttcgaggacagtt PRIMER_LEFT_0=21,20 PRIMER_RIGHT_0=214,20 PRIMER_LEFT_0_TM=59.006 PRIMER_RIGHT_0_TM=58.959 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY_TH=0.00 PRIMER_RIGHT_0_SELF_ANY_TH=16.51 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=11.79 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=0.00 PRIMER_LEFT_0_END_STABILITY=4.0000 PRIMER_RIGHT_0_END_STABILITY=3.1600 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=10.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=8.0000 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=194 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=18.00 PRIMER_PAIR_1_PENALTY=0.053176 PRIMER_LEFT_1_PENALTY=0.024271 PRIMER_RIGHT_1_PENALTY=0.028905 PRIMER_LEFT_1_SEQUENCE=gacacacttgctgctttcca PRIMER_RIGHT_1_SEQUENCE=atgtggaccgttgttgttgg PRIMER_LEFT_1=610,20 PRIMER_RIGHT_1=804,20 PRIMER_LEFT_1_TM=58.976 PRIMER_RIGHT_1_TM=58.971 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY_TH=0.00 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=0.00 PRIMER_LEFT_1_END_STABILITY=3.5300 PRIMER_RIGHT_1_END_STABILITY=3.7700 PRIMER_LEFT_1_TEMPLATE_MISPRIMING=8.0000 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING=11.0000 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=195 PRIMER_PAIR_1_TEMPLATE_MISPRIMING=19.00 PRIMER_PAIR_2_PENALTY=0.068003 PRIMER_LEFT_2_PENALTY=0.040646 PRIMER_RIGHT_2_PENALTY=0.027357 PRIMER_LEFT_2_SEQUENCE=aactgtcctcgaacagctca PRIMER_RIGHT_2_SEQUENCE=ccagccatcaataggtgcac PRIMER_LEFT_2=195,20 PRIMER_RIGHT_2=371,20 PRIMER_LEFT_2_TM=58.959 PRIMER_RIGHT_2_TM=58.973 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY_TH=5.32 PRIMER_RIGHT_2_SELF_ANY_TH=8.80 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=8.80 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=0.00 PRIMER_LEFT_2_END_STABILITY=4.2600 PRIMER_RIGHT_2_END_STABILITY=4.5700 PRIMER_LEFT_2_TEMPLATE_MISPRIMING=8.0000 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING=9.0000 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=177 PRIMER_PAIR_2_TEMPLATE_MISPRIMING=17.00 PRIMER_PAIR_3_PENALTY=0.082329 PRIMER_LEFT_3_PENALTY=0.028905 PRIMER_RIGHT_3_PENALTY=0.053424 PRIMER_LEFT_3_SEQUENCE=ccaacaacaacggtccacat PRIMER_RIGHT_3_SEQUENCE=aaccaaagcctctccacgta PRIMER_LEFT_3=785,20 PRIMER_RIGHT_3=996,20 PRIMER_LEFT_3_TM=58.971 PRIMER_RIGHT_3_TM=58.947 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY_TH=0.00 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=0.00 PRIMER_LEFT_3_END_STABILITY=3.2100 PRIMER_RIGHT_3_END_STABILITY=3.5700 PRIMER_LEFT_3_TEMPLATE_MISPRIMING=10.0000 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING=9.0000 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=212 PRIMER_PAIR_3_TEMPLATE_MISPRIMING=18.00 PRIMER_PAIR_4_PENALTY=0.106163 PRIMER_LEFT_4_PENALTY=0.081892 PRIMER_RIGHT_4_PENALTY=0.024271 PRIMER_LEFT_4_SEQUENCE=caacatggagagacttgccg PRIMER_RIGHT_4_SEQUENCE=tggaaagcagcaagtgtgtc PRIMER_LEFT_4=468,20 PRIMER_RIGHT_4=629,20 PRIMER_LEFT_4_TM=58.918 PRIMER_RIGHT_4_TM=58.976 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY_TH=0.00 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=0.00 PRIMER_LEFT_4_END_STABILITY=5.6900 PRIMER_RIGHT_4_END_STABILITY=3.6700 PRIMER_LEFT_4_TEMPLATE_MISPRIMING=7.0000 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING=9.0000 PRIMER_PAIR_4_COMPL_ANY_TH=1.86 PRIMER_PAIR_4_COMPL_END_TH=13.09 PRIMER_PAIR_4_PRODUCT_SIZE=162 PRIMER_PAIR_4_TEMPLATE_MISPRIMING=15.00 = primer3-2.3.6/test/primer_first_base_index_input0000664000077300007730000001167512231156245021077 0ustar ioanaioanaSEQUENCE_ID=negative first base index SEQUENCE_TEMPLATE=TTCTCATGTTTGACAGCTTATCATCGATAAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCAATTTCTATGCGCACCCGTTCTCGGAGCACTGTCCGACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTACTTGGAGCCACTATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA PRIMER_EXPLAIN_FLAG=1 PRIMER_FIRST_BASE_INDEX=-200 PRIMER_NUM_RETURN=5 SEQUENCE_INCLUDED_REGION=-200,4361 PRIMER_PRODUCT_SIZE_RANGE=100-300 PRIMER_MIN_THREE_PRIME_DISTANCE=0 PRIMER_OPT_SIZE=20 PRIMER_MIN_SIZE=18 PRIMER_MAX_SIZE=25 PRIMER_OPT_GC_PERCENT=50 PRIMER_MIN_GC=45 PRIMER_MAX_GC=55 PRIMER_OPT_TM=60 PRIMER_MIN_TM=55 PRIMER_MAX_TM=80 PRIMER_GC_CLAMP=0 PRIMER_PAIR_MAX_DIFF_TM=40 PRIMER_MAX_POLY_X=5 PRIMER_MAX_SELF_ANY_TH=47 PRIMER_MAX_SELF_END_TH=47 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47 PRIMER_LIBERAL_BASE=0 PRIMER_MAX_NS_ACCEPTED=8 PRIMER_DNA_CONC=50 PRIMER_DNTP_CONC=0.6 PRIMER_SALT_MONOVALENT=50 PRIMER_SALT_DIVALENT=1.5 = primer3-2.3.6/test/primer_mispriming_output0000664000077300007730000010660612231156245020145 0ustar ioanaioanaSEQUENCE_ID=exon1 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG SEQUENCE_EXCLUDED_REGION=100,150 PRIMER_PRODUCT_SIZE_RANGE=200-250 PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 PRIMER_PAIR_MAX_DIFF_TM=3 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_LEFT_EXPLAIN=considered 877, too many Ns 1, overlap excluded region 91, GC content failed 7, low tm 2, high tm 399, ok 377 PRIMER_RIGHT_EXPLAIN=considered 1012, too many Ns 19, overlap excluded region 58, GC content failed 25, high tm 702, ok 208 PRIMER_PAIR_EXPLAIN=considered 23, unacceptable product size 17, ok 6 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.917137 PRIMER_LEFT_0_PENALTY=2.223427 PRIMER_RIGHT_0_PENALTY=3.693710 PRIMER_LEFT_0_SEQUENCE=TCACCCACGAGTGAAGAC PRIMER_RIGHT_0_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_LEFT_0=43,18 PRIMER_RIGHT_0=279,18 PRIMER_LEFT_0_TM=55.223 PRIMER_RIGHT_0_TM=56.694 PRIMER_LEFT_0_GC_PERCENT=55.556 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=237 PRIMER_PAIR_1_PENALTY=5.931775 PRIMER_LEFT_1_PENALTY=2.238065 PRIMER_RIGHT_1_PENALTY=3.693710 PRIMER_LEFT_1_SEQUENCE=GTTTCACCCACGAGTGAA PRIMER_RIGHT_1_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_LEFT_1=40,18 PRIMER_RIGHT_1=279,18 PRIMER_LEFT_1_TM=55.238 PRIMER_RIGHT_1_TM=56.694 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=8.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=240 PRIMER_PAIR_2_PENALTY=5.947634 PRIMER_LEFT_2_PENALTY=2.253924 PRIMER_RIGHT_2_PENALTY=3.693710 PRIMER_LEFT_2_SEQUENCE=AGTTTCACCCACGAGTGA PRIMER_RIGHT_2_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_LEFT_2=39,18 PRIMER_RIGHT_2=279,18 PRIMER_LEFT_2_TM=54.746 PRIMER_RIGHT_2_TM=56.694 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_2_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=241 PRIMER_PAIR_3_PENALTY=5.947634 PRIMER_LEFT_3_PENALTY=2.253924 PRIMER_RIGHT_3_PENALTY=3.693710 PRIMER_LEFT_3_SEQUENCE=GAGTTTCACCCACGAGTG PRIMER_RIGHT_3_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_LEFT_3=38,18 PRIMER_RIGHT_3=279,18 PRIMER_LEFT_3_TM=54.746 PRIMER_RIGHT_3_TM=56.694 PRIMER_LEFT_3_GC_PERCENT=55.556 PRIMER_RIGHT_3_GC_PERCENT=61.111 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_3_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=242 PRIMER_PAIR_4_PENALTY=6.090290 PRIMER_LEFT_4_PENALTY=2.223427 PRIMER_RIGHT_4_PENALTY=3.866863 PRIMER_LEFT_4_SEQUENCE=TCACCCACGAGTGAAGAC PRIMER_RIGHT_4_SEQUENCE=GCTCCTCAGCCTTGTCAC PRIMER_LEFT_4=43,18 PRIMER_RIGHT_4=280,18 PRIMER_LEFT_4_TM=55.223 PRIMER_RIGHT_4_TM=56.867 PRIMER_LEFT_4_GC_PERCENT=55.556 PRIMER_RIGHT_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_4_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=238 = SEQUENCE_ID=exon2 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 1, long poly-x seq 23, ok 507 PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, ok 205 PRIMER_PAIR_EXPLAIN=considered 9, unacceptable product size 4, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.865499 PRIMER_LEFT_0_PENALTY=0.108160 PRIMER_RIGHT_0_PENALTY=3.757339 PRIMER_LEFT_0_SEQUENCE=CCCAAATCCCATTATTCATA PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_LEFT_0=39,20 PRIMER_RIGHT_0=267,23 PRIMER_LEFT_0_TM=54.892 PRIMER_RIGHT_0_TM=54.243 PRIMER_LEFT_0_GC_PERCENT=35.000 PRIMER_RIGHT_0_GC_PERCENT=30.435 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=229 PRIMER_PAIR_1_PENALTY=4.330804 PRIMER_LEFT_1_PENALTY=0.108160 PRIMER_RIGHT_1_PENALTY=4.222644 PRIMER_LEFT_1_SEQUENCE=CCCAAATCCCATTATTCATA PRIMER_RIGHT_1_SEQUENCE=ATGATGTCTACTTTCCAAATTACA PRIMER_LEFT_1=39,20 PRIMER_RIGHT_1=268,24 PRIMER_LEFT_1_TM=54.892 PRIMER_RIGHT_1_TM=54.777 PRIMER_LEFT_1_GC_PERCENT=35.000 PRIMER_RIGHT_1_GC_PERCENT=29.167 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_LEFT_1_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=5.9000 PRIMER_RIGHT_1_END_STABILITY=6.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=230 PRIMER_PAIR_2_PENALTY=4.567292 PRIMER_LEFT_2_PENALTY=0.809953 PRIMER_RIGHT_2_PENALTY=3.757339 PRIMER_LEFT_2_SEQUENCE=CTTAAGTTTGTAACAGGGCC PRIMER_RIGHT_2_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_LEFT_2=21,20 PRIMER_RIGHT_2=267,23 PRIMER_LEFT_2_TM=54.190 PRIMER_RIGHT_2_TM=54.243 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=30.435 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_2_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=12.4000 PRIMER_RIGHT_2_END_STABILITY=6.0000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=247 PRIMER_PAIR_3_PENALTY=4.567292 PRIMER_LEFT_3_PENALTY=0.809953 PRIMER_RIGHT_3_PENALTY=3.757339 PRIMER_LEFT_3_SEQUENCE=CCTTAAGTTTGTAACAGGGC PRIMER_RIGHT_3_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_LEFT_3=20,20 PRIMER_RIGHT_3=267,23 PRIMER_LEFT_3_TM=54.190 PRIMER_RIGHT_3_TM=54.243 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=30.435 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_3_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=6.0000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=248 PRIMER_PAIR_4_PENALTY=4.944651 PRIMER_LEFT_4_PENALTY=1.187311 PRIMER_RIGHT_4_PENALTY=3.757339 PRIMER_LEFT_4_SEQUENCE=ACCTTAAGTTTGTAACAGGGC PRIMER_RIGHT_4_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_LEFT_4=19,21 PRIMER_RIGHT_4=267,23 PRIMER_LEFT_4_TM=55.187 PRIMER_RIGHT_4_TM=54.243 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=30.435 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_4_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=10.9000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=249 = PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_ID=exon2 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 1, long poly-x seq 23, ok 507 PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, ok 205 PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 517, high tm 178, long poly-x seq 23, ok 2045 PRIMER_PAIR_EXPLAIN=considered 9, unacceptable product size 4, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.865499 PRIMER_LEFT_0_PENALTY=0.108160 PRIMER_RIGHT_0_PENALTY=3.757339 PRIMER_INTERNAL_0_PENALTY=0.055352 PRIMER_LEFT_0_SEQUENCE=CCCAAATCCCATTATTCATA PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_0_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_0=39,20 PRIMER_RIGHT_0=267,23 PRIMER_INTERNAL_0=127,20 PRIMER_LEFT_0_TM=54.892 PRIMER_RIGHT_0_TM=54.243 PRIMER_INTERNAL_0_TM=59.945 PRIMER_LEFT_0_GC_PERCENT=35.000 PRIMER_RIGHT_0_GC_PERCENT=30.435 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=229 PRIMER_PAIR_1_PENALTY=4.330804 PRIMER_LEFT_1_PENALTY=0.108160 PRIMER_RIGHT_1_PENALTY=4.222644 PRIMER_INTERNAL_1_PENALTY=0.055352 PRIMER_LEFT_1_SEQUENCE=CCCAAATCCCATTATTCATA PRIMER_RIGHT_1_SEQUENCE=ATGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_1_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_1=39,20 PRIMER_RIGHT_1=268,24 PRIMER_INTERNAL_1=127,20 PRIMER_LEFT_1_TM=54.892 PRIMER_RIGHT_1_TM=54.777 PRIMER_INTERNAL_1_TM=59.945 PRIMER_LEFT_1_GC_PERCENT=35.000 PRIMER_RIGHT_1_GC_PERCENT=29.167 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=5.9000 PRIMER_RIGHT_1_END_STABILITY=6.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=230 PRIMER_PAIR_2_PENALTY=4.567292 PRIMER_LEFT_2_PENALTY=0.809953 PRIMER_RIGHT_2_PENALTY=3.757339 PRIMER_INTERNAL_2_PENALTY=0.055352 PRIMER_LEFT_2_SEQUENCE=CTTAAGTTTGTAACAGGGCC PRIMER_RIGHT_2_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_2_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_2=21,20 PRIMER_RIGHT_2=267,23 PRIMER_INTERNAL_2=127,20 PRIMER_LEFT_2_TM=54.190 PRIMER_RIGHT_2_TM=54.243 PRIMER_INTERNAL_2_TM=59.945 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=30.435 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_2_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=12.4000 PRIMER_RIGHT_2_END_STABILITY=6.0000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=247 PRIMER_PAIR_3_PENALTY=4.567292 PRIMER_LEFT_3_PENALTY=0.809953 PRIMER_RIGHT_3_PENALTY=3.757339 PRIMER_INTERNAL_3_PENALTY=0.055352 PRIMER_LEFT_3_SEQUENCE=CCTTAAGTTTGTAACAGGGC PRIMER_RIGHT_3_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_3_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_3=20,20 PRIMER_RIGHT_3=267,23 PRIMER_INTERNAL_3=127,20 PRIMER_LEFT_3_TM=54.190 PRIMER_RIGHT_3_TM=54.243 PRIMER_INTERNAL_3_TM=59.945 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=30.435 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_3_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=6.0000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=248 PRIMER_PAIR_4_PENALTY=4.944651 PRIMER_LEFT_4_PENALTY=1.187311 PRIMER_RIGHT_4_PENALTY=3.757339 PRIMER_INTERNAL_4_PENALTY=0.055352 PRIMER_LEFT_4_SEQUENCE=ACCTTAAGTTTGTAACAGGGC PRIMER_RIGHT_4_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_4_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_4=19,21 PRIMER_RIGHT_4=267,23 PRIMER_INTERNAL_4=127,20 PRIMER_LEFT_4_TM=55.187 PRIMER_RIGHT_4_TM=54.243 PRIMER_INTERNAL_4_TM=59.945 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=30.435 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_4_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=10.9000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=249 = SEQUENCE_ID=exon1_2 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_INTERNAL_MIN_TM=50 PRIMER_INTERNAL_MAX_TM=70 PRIMER_EXPLAIN_FLAG=1 SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGGCGGCAGGCAGCAGTTCCCGACCGGCTCGAGGAGGAGCAGAGGCTGTGCTTGGCGCACCACTTCTGGGGCTGCTGTGAGGTCCGNCTGGAACCCGCTGCGCGGCTTCGAGTGGTCAAAGGAGCCAAAGATGAAGAGAAAAGGAAAACCAAGAGCGGGTGACAAGGCTGAGGAGCCCCGAAAGGCGGACCGTCAGGCTGAGGAGCGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG SEQUENCE_EXCLUDED_REGION=100,150 PRIMER_PRODUCT_SIZE_RANGE=200-250 PRIMER_MAX_TM=70 PRIMER_OPT_TM=55 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=12 PRIMER_MIN_TM=50 PRIMER_PAIR_MAX_DIFF_TM=3 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=16 PRIMER_LEFT_EXPLAIN=considered 877, too many Ns 1, overlap excluded region 91, GC content failed 7, low tm 2, high tm 399, ok 377 PRIMER_RIGHT_EXPLAIN=considered 1012, too many Ns 19, overlap excluded region 58, GC content failed 25, high tm 702, ok 208 PRIMER_INTERNAL_EXPLAIN=considered 3237, too many Ns 27, GC content failed 32, low tm 23, high tm 1880, ok 1275 PRIMER_PAIR_EXPLAIN=considered 26, unacceptable product size 17, high mispriming library similarity 2, ok 7 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=5 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.931775 PRIMER_LEFT_0_PENALTY=2.238065 PRIMER_RIGHT_0_PENALTY=3.693710 PRIMER_INTERNAL_0_PENALTY=0.010545 PRIMER_LEFT_0_SEQUENCE=GTTTCACCCACGAGTGAA PRIMER_RIGHT_0_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_INTERNAL_0_SEQUENCE=CTTCGAGTGGTCAAAGGAGC PRIMER_LEFT_0=40,18 PRIMER_RIGHT_0=279,18 PRIMER_INTERNAL_0=210,20 PRIMER_LEFT_0_TM=55.238 PRIMER_RIGHT_0_TM=56.694 PRIMER_INTERNAL_0_TM=59.989 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_INTERNAL_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_INTERNAL_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=8.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=240 PRIMER_PAIR_1_PENALTY=5.947634 PRIMER_LEFT_1_PENALTY=2.253924 PRIMER_RIGHT_1_PENALTY=3.693710 PRIMER_INTERNAL_1_PENALTY=0.010545 PRIMER_LEFT_1_SEQUENCE=GAGTTTCACCCACGAGTG PRIMER_RIGHT_1_SEQUENCE=CTCCTCAGCCTTGTCACC PRIMER_INTERNAL_1_SEQUENCE=CTTCGAGTGGTCAAAGGAGC PRIMER_LEFT_1=38,18 PRIMER_RIGHT_1=279,18 PRIMER_INTERNAL_1=210,20 PRIMER_LEFT_1_TM=54.746 PRIMER_RIGHT_1_TM=56.694 PRIMER_INTERNAL_1_TM=59.989 PRIMER_LEFT_1_GC_PERCENT=55.556 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_INTERNAL_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_INTERNAL_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=242 PRIMER_PAIR_2_PENALTY=6.090290 PRIMER_LEFT_2_PENALTY=2.223427 PRIMER_RIGHT_2_PENALTY=3.866863 PRIMER_INTERNAL_2_PENALTY=0.010545 PRIMER_LEFT_2_SEQUENCE=TCACCCACGAGTGAAGAC PRIMER_RIGHT_2_SEQUENCE=GCTCCTCAGCCTTGTCAC PRIMER_INTERNAL_2_SEQUENCE=CTTCGAGTGGTCAAAGGAGC PRIMER_LEFT_2=43,18 PRIMER_RIGHT_2=280,18 PRIMER_INTERNAL_2=210,20 PRIMER_LEFT_2_TM=55.223 PRIMER_RIGHT_2_TM=56.867 PRIMER_INTERNAL_2_TM=59.989 PRIMER_LEFT_2_GC_PERCENT=55.556 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_INTERNAL_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_INTERNAL_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_2_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=238 PRIMER_PAIR_3_PENALTY=6.104928 PRIMER_LEFT_3_PENALTY=2.238065 PRIMER_RIGHT_3_PENALTY=3.866863 PRIMER_INTERNAL_3_PENALTY=0.010545 PRIMER_LEFT_3_SEQUENCE=GTTTCACCCACGAGTGAA PRIMER_RIGHT_3_SEQUENCE=GCTCCTCAGCCTTGTCAC PRIMER_INTERNAL_3_SEQUENCE=CTTCGAGTGGTCAAAGGAGC PRIMER_LEFT_3=40,18 PRIMER_RIGHT_3=280,18 PRIMER_INTERNAL_3=210,20 PRIMER_LEFT_3_TM=55.238 PRIMER_RIGHT_3_TM=56.867 PRIMER_INTERNAL_3_TM=59.989 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=61.111 PRIMER_INTERNAL_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_INTERNAL_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=8.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_3_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=241 PRIMER_PAIR_4_PENALTY=6.120787 PRIMER_LEFT_4_PENALTY=2.253924 PRIMER_RIGHT_4_PENALTY=3.866863 PRIMER_INTERNAL_4_PENALTY=0.010545 PRIMER_LEFT_4_SEQUENCE=AGTTTCACCCACGAGTGA PRIMER_RIGHT_4_SEQUENCE=GCTCCTCAGCCTTGTCAC PRIMER_INTERNAL_4_SEQUENCE=CTTCGAGTGGTCAAAGGAGC PRIMER_LEFT_4=39,18 PRIMER_RIGHT_4=280,18 PRIMER_INTERNAL_4=210,20 PRIMER_LEFT_4_TM=54.746 PRIMER_RIGHT_4_TM=56.867 PRIMER_INTERNAL_4_TM=59.989 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=61.111 PRIMER_INTERNAL_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_INTERNAL_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_4_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=242 = SEQUENCE_ID=exon2_2 PRIMER_NUM_RETURN=7 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 14, long poly-x seq 23, ok 494 PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, high repeat similarity 19, ok 186 PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 517, high tm 178, long poly-x seq 23, ok 2045 PRIMER_PAIR_EXPLAIN=considered 167, unacceptable product size 101, tm diff too large 12, high mispriming library similarity 46, ok 8 PRIMER_LEFT_NUM_RETURNED=7 PRIMER_RIGHT_NUM_RETURNED=7 PRIMER_INTERNAL_NUM_RETURNED=7 PRIMER_PAIR_NUM_RETURNED=7 PRIMER_PAIR_0_PENALTY=4.997478 PRIMER_LEFT_0_PENALTY=1.240139 PRIMER_RIGHT_0_PENALTY=3.757339 PRIMER_INTERNAL_0_PENALTY=0.055352 PRIMER_LEFT_0_SEQUENCE=TAAGTTTGTAACAGGGCCC PRIMER_RIGHT_0_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_0_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_0=23,19 PRIMER_RIGHT_0=267,23 PRIMER_INTERNAL_0=127,20 PRIMER_LEFT_0_TM=55.240 PRIMER_RIGHT_0_TM=54.243 PRIMER_INTERNAL_0_TM=59.945 PRIMER_LEFT_0_GC_PERCENT=47.368 PRIMER_RIGHT_0_GC_PERCENT=30.435 PRIMER_INTERNAL_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_INTERNAL_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_INTERNAL_0_SELF_END=2.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=245 PRIMER_PAIR_1_PENALTY=5.462783 PRIMER_LEFT_1_PENALTY=1.240139 PRIMER_RIGHT_1_PENALTY=4.222644 PRIMER_INTERNAL_1_PENALTY=0.055352 PRIMER_LEFT_1_SEQUENCE=TAAGTTTGTAACAGGGCCC PRIMER_RIGHT_1_SEQUENCE=ATGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_1_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_1=23,19 PRIMER_RIGHT_1=268,24 PRIMER_INTERNAL_1=127,20 PRIMER_LEFT_1_TM=55.240 PRIMER_RIGHT_1_TM=54.777 PRIMER_INTERNAL_1_TM=59.945 PRIMER_LEFT_1_GC_PERCENT=47.368 PRIMER_RIGHT_1_GC_PERCENT=29.167 PRIMER_INTERNAL_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_INTERNAL_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_INTERNAL_1_SELF_END=2.00 PRIMER_LEFT_1_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_1_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_1_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_1_END_STABILITY=12.4000 PRIMER_RIGHT_1_END_STABILITY=6.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=246 PRIMER_PAIR_2_PENALTY=5.542868 PRIMER_LEFT_2_PENALTY=1.785529 PRIMER_RIGHT_2_PENALTY=3.757339 PRIMER_INTERNAL_2_PENALTY=0.055352 PRIMER_LEFT_2_SEQUENCE=TTAAGTTTGTAACAGGGCCC PRIMER_RIGHT_2_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_2_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_2=22,20 PRIMER_RIGHT_2=267,23 PRIMER_INTERNAL_2=127,20 PRIMER_LEFT_2_TM=56.786 PRIMER_RIGHT_2_TM=54.243 PRIMER_INTERNAL_2_TM=59.945 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=30.435 PRIMER_INTERNAL_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_INTERNAL_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_INTERNAL_2_SELF_END=2.00 PRIMER_LEFT_2_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_2_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_2_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_2_END_STABILITY=12.4000 PRIMER_RIGHT_2_END_STABILITY=6.0000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=246 PRIMER_PAIR_3_PENALTY=5.790774 PRIMER_LEFT_3_PENALTY=2.033435 PRIMER_RIGHT_3_PENALTY=3.757339 PRIMER_INTERNAL_3_PENALTY=0.055352 PRIMER_LEFT_3_SEQUENCE=AAGTTTGTAACAGGGCCC PRIMER_RIGHT_3_SEQUENCE=TGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_3_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_3=24,18 PRIMER_RIGHT_3=267,23 PRIMER_INTERNAL_3=127,20 PRIMER_LEFT_3_TM=55.033 PRIMER_RIGHT_3_TM=54.243 PRIMER_INTERNAL_3_TM=59.945 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=30.435 PRIMER_INTERNAL_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_INTERNAL_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_INTERNAL_3_SELF_END=2.00 PRIMER_LEFT_3_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_3_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_3_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_3_END_STABILITY=12.4000 PRIMER_RIGHT_3_END_STABILITY=6.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=244 PRIMER_PAIR_4_PENALTY=6.008172 PRIMER_LEFT_4_PENALTY=1.785529 PRIMER_RIGHT_4_PENALTY=4.222644 PRIMER_INTERNAL_4_PENALTY=0.055352 PRIMER_LEFT_4_SEQUENCE=TTAAGTTTGTAACAGGGCCC PRIMER_RIGHT_4_SEQUENCE=ATGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_4_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_4=22,20 PRIMER_RIGHT_4=268,24 PRIMER_INTERNAL_4=127,20 PRIMER_LEFT_4_TM=56.786 PRIMER_RIGHT_4_TM=54.777 PRIMER_INTERNAL_4_TM=59.945 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=29.167 PRIMER_INTERNAL_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_INTERNAL_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_INTERNAL_4_SELF_END=2.00 PRIMER_LEFT_4_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_4_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_4_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_4_END_STABILITY=12.4000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=247 PRIMER_PAIR_5_PENALTY=6.256078 PRIMER_LEFT_5_PENALTY=2.033435 PRIMER_RIGHT_5_PENALTY=4.222644 PRIMER_INTERNAL_5_PENALTY=0.055352 PRIMER_LEFT_5_SEQUENCE=AAGTTTGTAACAGGGCCC PRIMER_RIGHT_5_SEQUENCE=ATGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_5_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_5=24,18 PRIMER_RIGHT_5=268,24 PRIMER_INTERNAL_5=127,20 PRIMER_LEFT_5_TM=55.033 PRIMER_RIGHT_5_TM=54.777 PRIMER_INTERNAL_5_TM=59.945 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=29.167 PRIMER_INTERNAL_5_GC_PERCENT=40.000 PRIMER_LEFT_5_SELF_ANY=6.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_INTERNAL_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=6.00 PRIMER_RIGHT_5_SELF_END=6.00 PRIMER_INTERNAL_5_SELF_END=2.00 PRIMER_LEFT_5_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_5_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_5_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_5_END_STABILITY=12.4000 PRIMER_RIGHT_5_END_STABILITY=6.0000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PAIR_5_PRODUCT_SIZE=245 PRIMER_PAIR_6_PENALTY=6.277288 PRIMER_LEFT_6_PENALTY=1.240139 PRIMER_RIGHT_6_PENALTY=5.037148 PRIMER_INTERNAL_6_PENALTY=0.055352 PRIMER_LEFT_6_SEQUENCE=TAAGTTTGTAACAGGGCCC PRIMER_RIGHT_6_SEQUENCE=TATGATGTCTACTTTCCAAATTACA PRIMER_INTERNAL_6_SEQUENCE=GTTTGGCATGATTTTGCCTT PRIMER_LEFT_6=23,19 PRIMER_RIGHT_6=269,25 PRIMER_INTERNAL_6=127,20 PRIMER_LEFT_6_TM=55.240 PRIMER_RIGHT_6_TM=54.963 PRIMER_INTERNAL_6_TM=59.945 PRIMER_LEFT_6_GC_PERCENT=47.368 PRIMER_RIGHT_6_GC_PERCENT=28.000 PRIMER_INTERNAL_6_GC_PERCENT=40.000 PRIMER_LEFT_6_SELF_ANY=6.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_INTERNAL_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=6.00 PRIMER_RIGHT_6_SELF_END=6.00 PRIMER_INTERNAL_6_SELF_END=2.00 PRIMER_LEFT_6_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_RIGHT_6_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_PAIR_6_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy PRIMER_LEFT_6_END_STABILITY=12.4000 PRIMER_RIGHT_6_END_STABILITY=6.0000 PRIMER_PAIR_6_COMPL_ANY=6.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PAIR_6_PRODUCT_SIZE=247 = PRIMER_NUM_RETURN=5 PRIMER_MAX_LIBRARY_MISPRIMING=9 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_PICK_INTERNAL_OLIGO=1 SEQUENCE_ID=exon2_3 SEQUENCE_TEMPLATE=CTCCATGAAGTACAAAAAAACCTTAAGTTTGTAACAGGGCCCAAATCCCATTATTCATAATGCATTTCAAGGTAAAAATGAGGTATGAATACAATACCCTAACATCAATTCCTCCAACAAAAACAGTGTTTGGCATGATTTTGCCTTCTGGTAAAATATAGCCTTGGCTGGTTGCAGCTGATGAGGACTGGGTGCTGGCCTCTCTGGAGATGGTTGAGTTTGGAGTTTCAGGATTTGCAGTAGACTGTAATTTGGAAAGTAGACATCATAATTAGATACACAGGAAAAACCTATACATAATGTGAAATATAATTTTTGTATTTTAAGTATAAAATATTTTAT SEQUENCE_EXCLUDED_REGION=100,145 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=10 PRIMER_LEFT_EXPLAIN=considered 728, overlap excluded region 69, GC content failed 2, low tm 126, high repeat similarity 124, long poly-x seq 23, ok 384 PRIMER_RIGHT_EXPLAIN=considered 827, overlap excluded region 72, GC content failed 314, low tm 236, high repeat similarity 176, ok 29 PRIMER_INTERNAL_EXPLAIN=considered 3079, GC content failed 316, low tm 517, high tm 178, long poly-x seq 23, ok 2045 PRIMER_PAIR_EXPLAIN=considered 12459, unacceptable product size 6872, tm diff too large 4075, high mispriming library similarity 1512, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = primer3-2.3.6/test/primer_renewed_tasks_input0000664000077300007730000000514212231156245020415 0ustar ioanaioanaPRIMER_MAX_SELF_ANY=12 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_EXPLAIN_FLAG=1 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_ID=pcr_primers SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG SEQUENCE_TARGET=21,122 = SEQUENCE_ID=pcr_primers_and_io PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG = SEQUENCE_ID=left_only PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TARGET=100,20 SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC = SEQUENCE_ID=right_only PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=0 SEQUENCE_TARGET=90,10 SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC = SEQUENCE_ID=hyb_probe_only PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_LEFT_PRIMER=0 SEQUENCE_INTERNAL_EXCLUDED_REGION=90,20 SEQUENCE_TEMPLATE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC = SEQUENCE_ID=left_only PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG = SEQUENCE_ID=right_only PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG = SEQUENCE_ID=hyb_probe_only PRIMER_INTERNAL_MIN_SIZE=20 PRIMER_INTERNAL_MAX_SIZE=20 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=0 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG = primer3-2.3.6/test/primer_end_pathology_output0000664000077300007730000001064412231156245020617 0ustar ioanaioanaPRIMER_OPT_SIZE=20 PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000 PRIMER_MAX_GC=80.0 PRIMER_MAX_SIZE=25 PRIMER_MAX_TM=63.0 PRIMER_MIN_SIZE=18 PRIMER_MIN_GC=20 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=6.99 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=6.99 SEQUENCE_ID=14.RHAP64FE2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT SEQUENCE_INCLUDED_REGION=19,432 SEQUENCE_TARGET=44,46 SEQUENCE_TARGET=92,33 SEQUENCE_TARGET=140,26 SEQUENCE_TARGET=170,14 SEQUENCE_TARGET=211,20 SEQUENCE_TARGET=238,26 SEQUENCE_TARGET=336,14 PRIMER_LEFT_EXPLAIN=considered 443, too many Ns 288, low tm 128, high tm 9, high end compl 12, ok 6 PRIMER_RIGHT_EXPLAIN=considered 599, too many Ns 324, low tm 273, ok 2 PRIMER_PAIR_EXPLAIN=considered 41, unacceptable product size 36, ok 5 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.458561 PRIMER_LEFT_0_PENALTY=1.104149 PRIMER_RIGHT_0_PENALTY=5.354412 PRIMER_LEFT_0_SEQUENCE=TCCACATGTGCACAGTGGTAT PRIMER_RIGHT_0_SEQUENCE=GCCTGTGATGTATGTATGTGAGA PRIMER_LEFT_0=21,21 PRIMER_RIGHT_0=313,23 PRIMER_LEFT_0_TM=59.896 PRIMER_RIGHT_0_TM=57.646 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.8000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=293 PRIMER_PAIR_1_PENALTY=8.467518 PRIMER_LEFT_1_PENALTY=3.113106 PRIMER_RIGHT_1_PENALTY=5.354412 PRIMER_LEFT_1_SEQUENCE=CACATGTGCACAGTGGTATGT PRIMER_RIGHT_1_SEQUENCE=GCCTGTGATGTATGTATGTGAGA PRIMER_LEFT_1=23,21 PRIMER_RIGHT_1=313,23 PRIMER_LEFT_1_TM=57.887 PRIMER_RIGHT_1_TM=57.646 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=43.478 PRIMER_LEFT_1_SELF_ANY=10.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=5.6000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=291 PRIMER_PAIR_2_PENALTY=8.789928 PRIMER_LEFT_2_PENALTY=1.104149 PRIMER_RIGHT_2_PENALTY=7.685778 PRIMER_LEFT_2_SEQUENCE=TCCACATGTGCACAGTGGTAT PRIMER_RIGHT_2_SEQUENCE=ATAAATTCATTTTTGCCTGTCATAT PRIMER_LEFT_2=21,21 PRIMER_RIGHT_2=288,25 PRIMER_LEFT_2_TM=59.896 PRIMER_RIGHT_2_TM=57.314 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=24.000 PRIMER_LEFT_2_SELF_ANY=12.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.8000 PRIMER_RIGHT_2_END_STABILITY=5.8000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=268 PRIMER_PAIR_3_PENALTY=9.104196 PRIMER_LEFT_3_PENALTY=3.749784 PRIMER_RIGHT_3_PENALTY=5.354412 PRIMER_LEFT_3_SEQUENCE=CCACATGTGCACAGTGGTATGT PRIMER_RIGHT_3_SEQUENCE=GCCTGTGATGTATGTATGTGAGA PRIMER_LEFT_3=22,22 PRIMER_RIGHT_3=313,23 PRIMER_LEFT_3_TM=61.750 PRIMER_RIGHT_3_TM=57.646 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=12.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=5.6000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=292 PRIMER_PAIR_4_PENALTY=10.198358 PRIMER_LEFT_4_PENALTY=4.843946 PRIMER_RIGHT_4_PENALTY=5.354412 PRIMER_LEFT_4_SEQUENCE=TCCACATGTGCACAGTGGTATG PRIMER_RIGHT_4_SEQUENCE=GCCTGTGATGTATGTATGTGAGA PRIMER_LEFT_4=21,22 PRIMER_RIGHT_4=313,23 PRIMER_LEFT_4_TM=62.844 PRIMER_RIGHT_4_TM=57.646 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=12.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=5.6000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=293 = primer3-2.3.6/test/primer_thermod_align_input0000775000077300007730000003074012231156245020400 0ustar ioanaioanaPRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=0 PRIMER_SALT_DIVALENT=0.0 PRIMER_DNTP_CONC=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 SEQUENCE_ID=thermod_default PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=47.0 PRIMER_MAX_SELF_END_TH=47.0 PRIMER_MAX_HAIRPIN_TH=47.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0 PRIMER_PAIR_MAX_COMPL_END_TH=47.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0 PRIMER_INTERNAL_MAX_SELF_END_TH=47.0 PRIMER_INTERNAL_MAX_HAIRPIN_TH=47.0 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=47.0 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=47.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_WT_SELF_END_TH=0.0 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_WT_TEMPLATE_MISPRIMING_TH=0.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0 PRIMER_INTERNAL_WT_SELF_END_TH=0.0 PRIMER_INTERNAL_WT_HAIRPIN_TH=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_END_TH=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=0.0 P3_COMMENT=default values of parameters using thermodynamic approach SEQUENCE_TEMPLATE=AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGACTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGAGGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG = SEQUENCE_ID=any_1 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=10.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=any_salt PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=2.5 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=60.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=any_weight PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=2.5 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=47.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=47.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=47.0 PRIMER_WT_SELF_ANY_TH=1.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=0.1 PRIMER_PAIR_WT_COMPL_ANY_TH=0.7 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=any_2 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=2.5 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=5.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=5.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=5.0 PRIMER_WT_SELF_ANY_TH=1.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=1.0 PRIMER_PAIR_WT_COMPL_ANY_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=any_no_effect PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=2.5 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_ANY_TH=0.0 PRIMER_PAIR_MAX_COMPL_ANY_TH=0.0 PRIMER_INTERNAL_MAX_SELF_ANY_TH=0.0 PRIMER_WT_SELF_ANY_TH=0.0 PRIMER_INTERNAL_WT_SELF_ANY_TH=0.0 PRIMER_PAIR_WT_COMPL_ANY_TH=0.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=end_th1 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_END_TH=10.0 PRIMER_PAIR_MAX_COMPL_END_TH=10.0 PRIMER_INTERNAL_MAX_SELF_END_TH=10.0 PRIMER_WT_SELF_END_TH=0.0 PRIMER_INTERNAL_WT_SELF_END_TH=0.0 PRIMER_PAIR_WT_COMPL_END_TH=0.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=end_th2 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_SELF_END_TH=47.0 PRIMER_PAIR_MAX_COMPL_END_TH=47.0 PRIMER_INTERNAL_MAX_SELF_END_TH=47.0 PRIMER_WT_SELF_END_TH=0.1 PRIMER_INTERNAL_WT_SELF_END_TH=0.01 PRIMER_PAIR_WT_COMPL_END_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=hairpin1 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_HAIRPIN_TH=50.0 PRIMER_INTERNAL_MAX_HAIRPIN_TH=50.0 PRIMER_WT_HAIRPIN_TH=1.0 PRIMER_INTERNAL_WT_HAIRPIN_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=hairpin2 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_HAIRPIN_TH=40.0 PRIMER_INTERNAL_MAX_HAIRPIN_TH=40.0 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_INTERNAL_WT_HAIRPIN_TH=0.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=hairpin3 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_HAIRPIN_TH=0.0 PRIMER_INTERNAL_MAX_HAIRPIN_TH=47.0 PRIMER_PICK_ANYWAY=1 PRIMER_WT_HAIRPIN_TH=0.0 PRIMER_INTERNAL_WT_HAIRPIN_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=template1 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_MAX_TEMPLATE_MISPRIMING_TH=45.0 PRIMER_WT_TEMPLATE_MISPRIMING_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=template2 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=50.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=template2_and_repeat_library PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_MISPRIMING_LIBRARY=../src/humrep_and_simple.txt PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=50.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=1.0 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = SEQUENCE_ID=template3 PRIMER_TASK=pick_pcr_primers_and_hyb_probe PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0 PRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=5 PRIMER_PRODUCT_SIZE_RANGE=50-500 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=20 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100 SEQUENCE_TEMPLATE=AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATCAAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTGTGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGCCAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGTGAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGCCCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAGTGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG = primer3-2.3.6/test/repeat_lib50000664000077300007730000001600012231156245015150 0ustar ioanaioana>ALU--repbase Human ALU interspersed repetitive sequence - a consensus * 2.1 GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA >MIR--repbase mammalian-wide interspersed repeat (MIR) - a consensus * ab56 GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTC GAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTG TAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCG CCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA >MIR2--repbase Repetitive element - a consensus * 1 TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT >L1--repbase Human L1 interspersed repetitive sequence - full length copy GGCGGTGGAGCCAAGATGACCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGAC CATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACC ACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAA AA primer3-2.3.6/test/primer3web_v0_4_0_default_settings_output0000664000077300007730000002175512231156245023164 0ustar ioanaioanaP3_SETTINGS_FILE_USED=../primer3web_v0_4_0_default_settings.txt P3_FILE_TYPE=settings P3_FILE_ID=Default settings of primer3web version 0.4.0 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=0 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0 PRIMER_TASK=pick_detection_primers PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=5 PRIMER_PRODUCT_SIZE_RANGE=150-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000 PRIMER_PRODUCT_OPT_SIZE=0 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0 PRIMER_MIN_SIZE=18 PRIMER_INTERNAL_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_INTERNAL_OPT_SIZE=20 PRIMER_MAX_SIZE=27 PRIMER_INTERNAL_MAX_SIZE=27 PRIMER_WT_SIZE_LT=1.0 PRIMER_INTERNAL_WT_SIZE_LT=1.0 PRIMER_WT_SIZE_GT=1.0 PRIMER_INTERNAL_WT_SIZE_GT=1.0 PRIMER_MIN_GC=20.0 PRIMER_INTERNAL_MIN_GC=20.0 PRIMER_MAX_GC=80.0 PRIMER_INTERNAL_MAX_GC=80.0 PRIMER_WT_GC_PERCENT_LT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0 PRIMER_WT_GC_PERCENT_GT=0.0 PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0 PRIMER_GC_CLAMP=0 PRIMER_MAX_END_GC=5 PRIMER_MIN_TM=57.0 PRIMER_INTERNAL_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_INTERNAL_OPT_TM=60.0 PRIMER_MAX_TM=63.0 PRIMER_INTERNAL_MAX_TM=63.0 PRIMER_PAIR_MAX_DIFF_TM=100.0 PRIMER_WT_TM_LT=1.0 PRIMER_INTERNAL_WT_TM_LT=1.0 PRIMER_WT_TM_GT=1.0 PRIMER_INTERNAL_WT_TM_GT=1.0 PRIMER_PAIR_WT_DIFF_TM=0.0 PRIMER_PRODUCT_MIN_TM=-1000000.0 PRIMER_PRODUCT_OPT_TM=0.0 PRIMER_PRODUCT_MAX_TM=1000000.0 PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0 PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0 PRIMER_TM_FORMULA=0 PRIMER_SALT_MONOVALENT=50.0 PRIMER_INTERNAL_SALT_MONOVALENT=50.0 PRIMER_SALT_DIVALENT=0.0 PRIMER_INTERNAL_SALT_DIVALENT=0.0 PRIMER_DNTP_CONC=0.0 PRIMER_INTERNAL_DNTP_CONC=0.0 PRIMER_SALT_CORRECTIONS=0 PRIMER_DNA_CONC=50.0 PRIMER_INTERNAL_DNA_CONC=50.0 PRIMER_MAX_SELF_ANY=8.00 PRIMER_INTERNAL_MAX_SELF_ANY=12.00 PRIMER_PAIR_MAX_COMPL_ANY=8.00 PRIMER_WT_SELF_ANY=0.0 PRIMER_INTERNAL_WT_SELF_ANY=0.0 PRIMER_PAIR_WT_COMPL_ANY=0.0 PRIMER_MAX_SELF_END=3.00 PRIMER_INTERNAL_MAX_SELF_END=12.00 PRIMER_PAIR_MAX_COMPL_END=3.00 PRIMER_WT_SELF_END=0.0 PRIMER_INTERNAL_WT_SELF_END=0.0 PRIMER_PAIR_WT_COMPL_END=0.0 PRIMER_MAX_END_STABILITY=9.0 PRIMER_WT_END_STABILITY=0.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_INTERNAL_MAX_NS_ACCEPTED=0 PRIMER_WT_NUM_NS=0.0 PRIMER_INTERNAL_WT_NUM_NS=0.0 PRIMER_MAX_POLY_X=5 PRIMER_INTERNAL_MAX_POLY_X=5 PRIMER_MIN_THREE_PRIME_DISTANCE=-1 PRIMER_PICK_ANYWAY=1 PRIMER_LOWERCASE_MASKING=0 PRIMER_EXPLAIN_FLAG=1 PRIMER_LIBERAL_BASE=1 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MAX_TEMPLATE_MISPRIMING=12.00 PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=24.00 PRIMER_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_MAX_LIBRARY_MISPRIMING=12.00 PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00 PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=24.00 PRIMER_WT_LIBRARY_MISPRIMING=0.0 PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0 PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0 PRIMER_MIN_QUALITY=0 PRIMER_INTERNAL_MIN_QUALITY=0 PRIMER_MIN_END_QUALITY=0 PRIMER_QUALITY_RANGE_MIN=0 PRIMER_QUALITY_RANGE_MAX=100 PRIMER_WT_SEQ_QUAL=0.0 PRIMER_INTERNAL_WT_SEQ_QUAL=0.0 PRIMER_PAIR_WT_PR_PENALTY=1.0 PRIMER_PAIR_WT_IO_PENALTY=0.0 PRIMER_INSIDE_PENALTY=-1.0 PRIMER_OUTSIDE_PENALTY=0.0 PRIMER_WT_POS_PENALTY=0.0 PRIMER_SEQUENCING_LEAD=50 PRIMER_SEQUENCING_SPACING=500 PRIMER_SEQUENCING_INTERVAL=250 PRIMER_SEQUENCING_ACCURACY=20 PRIMER_WT_END_QUAL=0.0 PRIMER_INTERNAL_WT_END_QUAL=0.0 P3_SETTINGS_FILE_END= SEQUENCE_ID=test_settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_LEFT_EXPLAIN=considered 12229, GC content failed 3, low tm 2664, high tm 6186, long poly-x seq 38, high 3' stability 222, ok 3116 PRIMER_RIGHT_EXPLAIN=considered 12305, GC content failed 3, low tm 2880, high tm 5939, long poly-x seq 43, high 3' stability 212, ok 3228 PRIMER_PAIR_EXPLAIN=considered 60, unacceptable product size 54, ok 6 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.037132 PRIMER_LEFT_0_PENALTY=0.031043 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_LEFT_0_SEQUENCE=actggtctcccatcgtcaac PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_0=107,20 PRIMER_RIGHT_0=316,20 PRIMER_LEFT_0_TM=59.969 PRIMER_RIGHT_0_TM=60.006 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_LEFT_0_TEMPLATE_MISPRIMING=10.0000 PRIMER_RIGHT_0_TEMPLATE_MISPRIMING=8.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=210 PRIMER_PAIR_0_TEMPLATE_MISPRIMING=17.00 PRIMER_PAIR_1_PENALTY=0.050921 PRIMER_LEFT_1_PENALTY=0.044832 PRIMER_RIGHT_1_PENALTY=0.006089 PRIMER_LEFT_1_SEQUENCE=cgaggattatggttgcacct PRIMER_RIGHT_1_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_1=81,20 PRIMER_RIGHT_1=316,20 PRIMER_LEFT_1_TM=59.955 PRIMER_RIGHT_1_TM=60.006 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_LEFT_1_TEMPLATE_MISPRIMING=9.0000 PRIMER_RIGHT_1_TEMPLATE_MISPRIMING=8.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=236 PRIMER_PAIR_1_TEMPLATE_MISPRIMING=17.00 PRIMER_PAIR_2_PENALTY=0.052224 PRIMER_LEFT_2_PENALTY=0.000037 PRIMER_RIGHT_2_PENALTY=0.052187 PRIMER_LEFT_2_SEQUENCE=atcgccaccgtacaagaaac PRIMER_RIGHT_2_SEQUENCE=cggtgcacggtaactaaggt PRIMER_LEFT_2=850,20 PRIMER_RIGHT_2=1041,20 PRIMER_LEFT_2_TM=60.000 PRIMER_RIGHT_2_TM=60.052 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_LEFT_2_TEMPLATE_MISPRIMING=8.0000 PRIMER_RIGHT_2_TEMPLATE_MISPRIMING=7.0000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=192 PRIMER_PAIR_2_TEMPLATE_MISPRIMING=15.00 PRIMER_PAIR_3_PENALTY=0.065561 PRIMER_LEFT_3_PENALTY=0.006937 PRIMER_RIGHT_3_PENALTY=0.058624 PRIMER_LEFT_3_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_3_SEQUENCE=agccggatcaatatcactcg PRIMER_LEFT_3=199,20 PRIMER_RIGHT_3=434,20 PRIMER_LEFT_3_TM=59.993 PRIMER_RIGHT_3_TM=60.059 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_LEFT_3_TEMPLATE_MISPRIMING=7.0000 PRIMER_RIGHT_3_TEMPLATE_MISPRIMING=8.0000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=236 PRIMER_PAIR_3_TEMPLATE_MISPRIMING=15.00 PRIMER_PAIR_4_PENALTY=0.073199 PRIMER_LEFT_4_PENALTY=0.066262 PRIMER_RIGHT_4_PENALTY=0.006937 PRIMER_LEFT_4_SEQUENCE=gccatcaatgacctccactt PRIMER_RIGHT_4_SEQUENCE=ggactgagctgttcgaggac PRIMER_LEFT_4=22,20 PRIMER_RIGHT_4=218,20 PRIMER_LEFT_4_TM=59.934 PRIMER_RIGHT_4_TM=59.993 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_LEFT_4_TEMPLATE_MISPRIMING=9.0000 PRIMER_RIGHT_4_TEMPLATE_MISPRIMING=8.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=197 PRIMER_PAIR_4_TEMPLATE_MISPRIMING=16.00 = primer3-2.3.6/test/oligotm_test.pl0000664000077300007730000000763612231156245016117 0ustar ioanaioana# Copyright (c) 2007,2010,2011 # Triinu Koressaar and Maido Remm # All rights reserved. # # This file is part of primer3, the libprimer3 library, the oligotm # library and the dpal library. # # Primer3 and the libraries above are free software; you can # redistribute them and/or modify them under the terms of the GNU # General Public License as published by the Free Software Foundation; # either version 2 of the License, or (at your option) any later # version. # # This software is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # # You should have received a copy of the GNU General Public License # along with this file (file gpl-2.0.txt in the source distribution); if # not, write to the Free Software Foundation, Inc., 51 Franklin St, # Fifth Floor, Boston, MA 02110-1301 USA # # THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS # "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT # LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR # A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT # OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, # SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT # LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, # DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY # THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT # (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE # OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. use strict; use warnings 'all'; use Getopt::Long; use Carp; use constant EPSILON => 1e-5; our $winFlag; our $exe = '../src/oligotm'; my $do_valgrind; my %args; print "\nTESTING Tm CALCULATIONS\n\n"; if (!GetOptions(\%args, 'valgrind', 'windows', )) { print "Usage: $0 [ --valgrind ] [ --windows ]\n"; exit -1; } $winFlag = defined $args{'windows'}; $do_valgrind = $args{'valgrind'}; if ($winFlag && $do_valgrind) { print "$0: Cannot specify both --valgrind and --windows\n"; exit -1; } my $valgrind_exe = "/usr/local/bin/valgrind"; if ((!-x $valgrind_exe) && ($do_valgrind)) { warn "Cannot find $valgrind_exe; will try `which valgrind`\n"; $valgrind_exe= `which valgrind`; chomp($valgrind_exe); if (!$valgrind_exe || ! -x $valgrind_exe) { die "Cannot execute $valgrind_exe"; } } my $valgrind_format = "$valgrind_exe --leak-check=yes " . " --show-reachable=yes --log-file=oligotm.%0.3d.valg "; my $nr=1; my $failure=0; if ($winFlag) { $exe = '..\\src\\oligotm.exe'; } die "Cannot execute $exe" unless -x $exe; print "Tm tests nr $nr-"; open F, "oligotm.txt" or die "Cannot open oligotm.txt\n"; while(){ chomp; next if $. == 1; my @tmp=split(/\t/); my $valgrind_prefix = $do_valgrind ? sprintf($valgrind_format, $nr) : ''; my $cmd = "$valgrind_prefix " . " $exe -tp $tmp[1] -sc $tmp[2] -mv $tmp[3] " . " -dv $tmp[4] -n $tmp[5] $tmp[0] |"; open(CMD, $cmd); # execute the command and take the output my $tm=; chomp $tm; close(CMD); if($tm){ if(abs($tm-$tmp[6])>EPSILON) { $failure++; print "$cmd FAILED (expected $tm, got $tmp[6])\n"; } } else { $failure++; print "$cmd FAILED (no output)\n"; } $nr++; } close F; $nr--; if ($do_valgrind) { my $r = system "grep ERROR *.valg.* | grep -v 'ERROR SUMMARY: 0 errors'"; if (!$r) { # !$r because grep returns 0 if something is found, # and if something is found, we have a problem. print "valgrind found errors\n"; } } if(!$failure){ print "$nr: OK\n\n\n"; } else{ print "\n$nr Tm tests: $failure FAILURES\n\n\n"; } primer3-2.3.6/test/primer_overlap_junction_output0000664000077300007730000002214712231156245021345 0ustar ioanaioanaSEQUENCE_ID=Details of SEQUENCE_OVERLAP_JUNCTION_LIST and PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION TEST 01 -- OVERLAP P3_COMMENT=The 50th nt is uppercase PRIMER_NUM_RETURN=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_PRODUCT_SIZE_RANGE=40-100 PRIMER_MIN_GC=0 PRIMER_MIN_TM=0 PRIMER_PICK_ANYWAY=0 SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION=2 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=2 SEQUENCE_OVERLAP_JUNCTION_LIST=50 PRIMER_TASK=pick_detection_primers PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_MAX_POLY_X=100 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_SIZE=16 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAga PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=22.403937 PRIMER_LEFT_0_PENALTY=8.942519 PRIMER_RIGHT_0_PENALTY=13.461417 PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAga PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=65,18 PRIMER_LEFT_0_TM=51.057 PRIMER_RIGHT_0_TM=48.539 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=27.778 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=65 = SEQUENCE_ID=Details of SEQUENCE_OVERLAP_JUNCTION_LIST and PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION TEST 02 -- OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccAg PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=25.992387 PRIMER_LEFT_0_PENALTY=8.942519 PRIMER_RIGHT_0_PENALTY=17.049867 PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa PRIMER_RIGHT_0_SEQUENCE=aaaaaaacacaatccAg PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=65,17 PRIMER_LEFT_0_TM=51.057 PRIMER_RIGHT_0_TM=45.950 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=29.412 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=65 = SEQUENCE_ID=Details of SEQUENCE_OVERLAP_JUNCTION_LIST and PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION TEST 03 -- NO OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=aaaaaaacacaatccA PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no overlap of required point 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=Details of SEQUENCE_OVERLAP_JUNCTION_LIST and PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION TEST 08 -- OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=ttgttcattctgtttcTgg SEQUENCE_PRIMER_REVCOMP=catcacacccttttacacatt PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=15.138591 PRIMER_LEFT_0_PENALTY=9.157204 PRIMER_RIGHT_0_PENALTY=5.981388 PRIMER_LEFT_0_SEQUENCE=ttgttcattctgtttcTgg PRIMER_RIGHT_0_SEQUENCE=catcacacccttttacacatt PRIMER_LEFT_0=34,19 PRIMER_RIGHT_0=100,21 PRIMER_LEFT_0_TM=51.843 PRIMER_RIGHT_0_TM=55.019 PRIMER_LEFT_0_GC_PERCENT=36.842 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=67 = SEQUENCE_ID=Details of SEQUENCE_OVERLAP_JUNCTION_LIST and PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION TEST 09 -- NO OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=ttgttcattctgtttcTg SEQUENCE_PRIMER_REVCOMP=catcacacccttttacacatt PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no overlap of required point 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=Test for 5' overlap on reverse primer -- OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=3 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=tccAgaaacagaatgaacaatag PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=15.822707 PRIMER_LEFT_0_PENALTY=8.942519 PRIMER_RIGHT_0_PENALTY=6.880188 PRIMER_LEFT_0_SEQUENCE=cttgattcaatgaatctcaa PRIMER_RIGHT_0_SEQUENCE=tccAgaaacagaatgaacaatag PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=53,23 PRIMER_LEFT_0_TM=51.057 PRIMER_RIGHT_0_TM=56.120 PRIMER_LEFT_0_GC_PERCENT=30.000 PRIMER_RIGHT_0_GC_PERCENT=34.783 PRIMER_LEFT_0_SELF_ANY=11.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=5.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=53 = SEQUENCE_ID=Test for 5' overlap on reverse primer -- NO OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=ccAgaaacagaatgaacaatag PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no overlap of required point 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=Test for 5' overlap on reverse primer -- NO OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=cttgattcaatgaatctcaa SEQUENCE_PRIMER_REVCOMP=cAgaaacagaatgaacaatag PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no overlap of required point 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=Test for 5' overlap on forward primer -- OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=tcTggattgtgtttttttc SEQUENCE_PRIMER_REVCOMP=catcacacccttttacacatt PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=16.029050 PRIMER_LEFT_0_PENALTY=10.047662 PRIMER_RIGHT_0_PENALTY=5.981388 PRIMER_LEFT_0_SEQUENCE=tcTggattgtgtttttttc PRIMER_RIGHT_0_SEQUENCE=catcacacccttttacacatt PRIMER_LEFT_0=48,19 PRIMER_RIGHT_0=100,21 PRIMER_LEFT_0_TM=50.952 PRIMER_RIGHT_0_TM=55.019 PRIMER_LEFT_0_GC_PERCENT=31.579 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=53 = SEQUENCE_ID=Test for 5' overlap on forward primer -- NO OVERLAP SEQUENCE_TEMPLATE=cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatg SEQUENCE_OVERLAP_JUNCTION_LIST=50 SEQUENCE_PRIMER=cTggattgtgtttttttc SEQUENCE_PRIMER_REVCOMP=catcacacccttttacacatt PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 1, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, no overlap of required point 1, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = primer3-2.3.6/test/primer_three_prime_distance_input0000664000077300007730000000724612231156245021743 0ustar ioanaioanaSEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1 - 11 possible pairs PRIMER_PRODUCT_SIZE_RANGE=100-500 PRIMER_MIN_TM=0 PRIMER_MAX_TM=100 PRIMER_NUM_RETURN=40 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT SEQUENCE_EXCLUDED_REGION=21,89 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1 SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC = SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=0 - same results as for -1 SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT SEQUENCE_EXCLUDED_REGION=21,89 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=0 SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC = SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 - same results as for 0 and -1 SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT SEQUENCE_EXCLUDED_REGION=21,89 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC = SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=2 - 5 pairs SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT SEQUENCE_EXCLUDED_REGION=21,89 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=2 SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC = SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=3 - 4 pairs SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT SEQUENCE_EXCLUDED_REGION=21,89 PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=3 SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC = SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1 - 11 possible pairs SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC SEQUENCE_EXCLUDED_REGION=31,89 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1 SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA = SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=0 - same results as for -1 SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC SEQUENCE_EXCLUDED_REGION=31,89 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=0 SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA = SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 - same results as for 0 and -1 SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC SEQUENCE_EXCLUDED_REGION=31,89 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA = SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=2 - 5 pairs SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC SEQUENCE_EXCLUDED_REGION=31,89 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=2 SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA = SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=3 - 3 pairs SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC SEQUENCE_EXCLUDED_REGION=31,89 PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=3 SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA =primer3-2.3.6/test/primer_lib_amb_codes_input0000664000077300007730000001623512231156245020326 0ustar ioanaioanaPRIMER_EXPLAIN_FLAG=1 PRIMER_NUM_RETURN=1 PRIMER_OPT_SIZE=20 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_INTERNAL_MAX_SELF_END=8 PRIMER_INTERNAL_MAX_TM=100 PRIMER_INTERNAL_MIN_TM=0 PRIMER_MIN_SIZE=18 PRIMER_MAX_SIZE=25 PRIMER_OPT_TM=60.0 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_GC=0.0 PRIMER_MAX_GC=100.0 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_SALT_MONOVALENT=50.0 PRIMER_DNA_CONC=50.0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=8 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=8 PRIMER_PRODUCT_SIZE_RANGE=75-500 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib SEQUENCE_ID=test_01 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Should succeed = PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 SEQUENCE_ID=test_02 SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Should still succeed = SEQUENCE_ID=test_03 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG SEQUENCE_PRIMER=CGACTCTAGAGGATCCCCCT SEQUENCE_PRIMER_REVCOMP=TAGCCTGCCTTTGCAAACTT SEQUENCE_INTERNAL_OLIGO=GGAAGAGAGGGTCATGCAAC PRIMER_PICK_ANYWAY=1 P3_COMMENT=Both primers should have high repeat similarity = SEQUENCE_ID=test_04 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG SEQUENCE_PRIMER=CGACTCTAGAGGATCCCCCT SEQUENCE_PRIMER_REVCOMP=TAGCCTGCCTTTGCAAACTT SEQUENCE_INTERNAL_OLIGO=GGAAGAGAGGGTCATGCAAC PRIMER_PICK_ANYWAY=1 P3_COMMENT=Should succeed and both primers should have low repeat similarity = SEQUENCE_ID=test_05 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Both primers should have high repeat similarity, but the explain line for right primers will not indicate this because mispriming is calculated "lazily" -- only if there are good left primers. = SEQUENCE_ID=test_06 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_INTERNAL_MISHYB_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG SEQUENCE_PRIMER=CGACTCTAGAGGATCCCCCT SEQUENCE_PRIMER_REVCOMP=TAGCCTGCCTTTGCAAACTT SEQUENCE_INTERNAL_OLIGO=GGAAGAGAGGGTCATGCAAC PRIMER_PICK_ANYWAY=1 P3_COMMENT=Primers should be ok, but not internal oligo = SEQUENCE_ID=test_07 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_INTERNAL_MISHYB_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG PRIMER_PICK_ANYWAY=1 P3_COMMENT=Primers should be ok, but not internal oligo = SEQUENCE_ID=test_08 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MISPRIMING_LIBRARY=not_for_production.lib PRIMER_INTERNAL_MISHYB_LIBRARY=not_for_production_Ns.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGATCATCTTCTGATCCAAGACCCGTGCTAGTCTACTGTATTCTACATGTACCCTTCTACAAGCTTTGAATTCCATAATCATCAGAGAATCCAGAAATTATTCATTATCACATAATAACAACACTCAAATATTTAAAGTTTTATGGTTGAATCTTTATGATTGAACCCATTCTATCAGGTGGTTATTTTATATCTAATAGAATTCATTTCAGGGTTACACAAAGGTCCGAATCTATCCATTTTACTGAG P3_COMMENT=Should fail because of high repeat similarity for all internal oligos = SEQUENCE_ID=test_09 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0 PRIMER_MISPRIMING_LIBRARY=not_for_production_Xs.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Should succeed = SEQUENCE_ID=test_10 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MISPRIMING_LIBRARY=not_for_production_Xs.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Should fail (Xs are treated as Ns). = SEQUENCE_ID=test_11 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 PRIMER_MISPRIMING_LIBRARY= PRIMER_INTERNAL_MISHYB_LIBRARY=not_for_production_Xs.lib SEQUENCE_TEMPLATE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG P3_COMMENT=Should fail (Xs are treated as Ns). = primer3-2.3.6/test/primer_mispriming_long_lib_output0000664000077300007730000000403212231156245022000 0ustar ioanaioanaSEQUENCE_ID=seq0 P3_COMMENT=should fail PRIMER_MAX_LIBRARY_MISPRIMING=18 PRIMER_MAX_SELF_ANY=20 PRIMER_MAX_SELF_END=20 PRIMER_PAIR_MAX_COMPL_ANY=20 PRIMER_PAIR_MAX_COMPL_END=20 PRIMER_MAX_TM=100 PRIMER_MIN_TM=10 PRIMER_MIN_SIZE=20 PRIMER_MAX_SIZE=20 PRIMER_MIN_GC=10 PRIMER_MAX_GC=90 SEQUENCE_TEMPLATE=NACGGGCATGGCGATCGCTGGNNNNNNNNNNNNANNNNNNNNCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNANNNNNNNNCNNNNNNNNTNCNGCTGGCAGTCTAGCGGGCATNANANANANAAN PRIMER_MISPRIMING_LIBRARY=primer_repeat_long_lib PRIMER_EXPLAIN_FLAG=1 PRIMER_LEFT_EXPLAIN=considered 49, too many Ns 48, high repeat similarity 1, ok 0 PRIMER_RIGHT_EXPLAIN=considered 49, too many Ns 48, ok 1 PRIMER_PAIR_EXPLAIN=considered 0, ok 0 PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=seq1 P3_COMMENT=Should find one pair SEQUENCE_TEMPLATE=NACGGGCATGGCGATCGCTAANNNNNNNNNNNNANNNNNNNNCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNANNNNNNNNCNNNNNNNNTNCNGCTGGCAGTCTAGCGGGCATNANANANANAAN PRIMER_LEFT_EXPLAIN=considered 49, too many Ns 48, ok 1 PRIMER_RIGHT_EXPLAIN=considered 49, too many Ns 48, ok 1 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_LEFT_NUM_RETURNED=1 PRIMER_RIGHT_NUM_RETURNED=1 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=1 PRIMER_PAIR_0_PENALTY=17.167250 PRIMER_LEFT_0_PENALTY=10.043573 PRIMER_RIGHT_0_PENALTY=7.123677 PRIMER_LEFT_0_SEQUENCE=ACGGGCATGGCGATCGCTAA PRIMER_RIGHT_0_SEQUENCE=ATGCCCGCTAGACTGCCAGC PRIMER_LEFT_0=1,20 PRIMER_RIGHT_0=135,20 PRIMER_LEFT_0_TM=70.044 PRIMER_RIGHT_0_TM=67.124 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=65.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_LIBRARY_MISPRIMING=15.00, seq1099 * 1.0 PRIMER_RIGHT_0_LIBRARY_MISPRIMING=6.00, seq9 * 2.0 PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, seq1099 * 1.0 PRIMER_LEFT_0_END_STABILITY=7.5000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=135 = primer3-2.3.6/test/test_left_to_right_of_right_input0000664000077300007730000000226012231156245021750 0ustar ioanaioanaP3_COMMENT=The SEQUENCE_PRIMER and SEQUENCE_PRIMER_REVCOMP are reverse compliments of each other, and each is found at two positions in the template. The causes there to be 4 pairs to be considered, including one in which the left primer it to the right of the right primer. SEQUENCE_TEMPLATE=AGGCTAGGCGAGCTGAAAAATCCTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGCTAGGCGAGCTGAAAAATCCTAC PRIMER_MAX_TEMPLATE_MISPRIMING=12 PRIMER_PICK_ANYWAY=1 PRIMER_PRODUCT_SIZE_RANGE=30-100 PRIMER_GC_CLAMP=0 PRIMER_FIRST_BASE_INDEX=1 PRIMER_MIN_SIZE=19 PRIMER_TASK=pick_pcr_primers PRIMER_MIN_GC=18 PRIMER_MAX_NS_ACCEPTED=1 PRIMER_MIN_TM=50 PRIMER_EXPLAIN_FLAG=1 PRIMER_OPT_SIZE=20 PRIMER_NUM_RETURN=1 PRIMER_MAX_SIZE=27 PRIMER_TM_FORMULA=1 SEQUENCE_PRIMER=AGGCTAGGCGAGCTGAAAAATCCTAC SEQUENCE_PRIMER_REVCOMP=GTAGGATTTTTCAGCTCGCCTAGCCT PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=1 PRIMER_THERMODYNAMIC_PARAMETERS_PATH=./primer3_config/ PRIMER_SALT_CORRECTIONS=1 PRIMER_MAX_HAIRPIN_TH=47 PRIMER_MAX_SELF_ANY_TH=47 PRIMER_MAX_SELF_END_TH=47 PRIMER_PAIR_MAX_COMPL_ANY_TH=30 = primer3-2.3.6/test/primer_all_settingsfiles_output0000664000077300007730000001144112231156245021472 0ustar ioanaioanaSEQUENCE_ID=Load qPCR settings SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_PICK_LEFT_PRIMER=1 PRIMER_NUM_RETURN=5 PRIMER_TASK=pick_detection_primers PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.013026 PRIMER_LEFT_0_PENALTY=0.006937 PRIMER_RIGHT_0_PENALTY=0.006089 PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_0=198,20 PRIMER_RIGHT_0=315,20 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.006 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=118 PRIMER_PAIR_1_PENALTY=0.020508 PRIMER_LEFT_1_PENALTY=0.014419 PRIMER_RIGHT_1_PENALTY=0.006089 PRIMER_LEFT_1_SEQUENCE=ttctctggacacagctccct PRIMER_RIGHT_1_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_1=39,20 PRIMER_RIGHT_1=315,20 PRIMER_LEFT_1_TM=59.986 PRIMER_RIGHT_1_TM=60.006 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=277 PRIMER_PAIR_2_PENALTY=0.020508 PRIMER_LEFT_2_PENALTY=0.014419 PRIMER_RIGHT_2_PENALTY=0.006089 PRIMER_LEFT_2_SEQUENCE=cttctctggacacagctccc PRIMER_RIGHT_2_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_2=38,20 PRIMER_RIGHT_2=315,20 PRIMER_LEFT_2_TM=59.986 PRIMER_RIGHT_2_TM=60.006 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=278 PRIMER_PAIR_3_PENALTY=0.032388 PRIMER_LEFT_3_PENALTY=0.032351 PRIMER_RIGHT_3_PENALTY=0.000037 PRIMER_LEFT_3_SEQUENCE=gacacacttgctgctttcca PRIMER_RIGHT_3_SEQUENCE=gtttcttgtacggtggcgat PRIMER_LEFT_3=609,20 PRIMER_RIGHT_3=868,20 PRIMER_LEFT_3_TM=60.032 PRIMER_RIGHT_3_TM=60.000 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=9.8000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=260 PRIMER_PAIR_4_PENALTY=0.037132 PRIMER_LEFT_4_PENALTY=0.031043 PRIMER_RIGHT_4_PENALTY=0.006089 PRIMER_LEFT_4_SEQUENCE=actggtctcccatcgtcaac PRIMER_RIGHT_4_SEQUENCE=gcgtttttattgccgttgtt PRIMER_LEFT_4=106,20 PRIMER_RIGHT_4=315,20 PRIMER_LEFT_4_TM=59.969 PRIMER_RIGHT_4_TM=60.006 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=210 = primer3-2.3.6/test/not_for_production.lib0000664000077300007730000001611512231156245017445 0ustar ioanaioana>ALU Human ALU interspersed repetitive sequence - a consensus DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase. GGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAA >MIR mammalian-wide interspersed repeat (MIR) - a consensus ACAGYAYAGCATAGTGGTTAAGAGCACGGRCTCTGGAGCCAGACTGCCTGGGTTCGAATCCCGGCTCTGCCACTTACTAGCTGTGTGACCTTGGGCAAGTTACTTAACCTCTCTGTGCCTCAGTTTCCTCATCTGTAAAATGGGGATAATAATAGTACCTACCTCATAGGGTTGTTGTGAGGATTAAATGAGTTAATAYATGTAAAGCGCTTAGAACAGTGCCTGGCACATAGTAAGCGCTCAATAAATGTTRGYTATTATT >MIR2 Repetitive element - a consensus TTNTTTATTGTCTGTCTCCTCCACTAGANTGTAAGCTCCATGAGGGCAGGGATTTTGTCTGTYTTGTTCACTGCTGTATCCCCAGCGCCTAGAACAGTGCCTGGCACATAGTAGGCGCTCAATAAATATTTGTTGAATGAATGAATGAAT >L1 Human L1 interspersed repetitive sequence - full length copy GGCGGTGGAGCCAAGATGACCGAATAGGAACAGCTCCAGTCTATAGCTCCCATCGTGAGTGACGCAGAAGACGGGTGATTTCTGCATTTCCAACTGAGGTACCAGGTTCATCTCACAGGGAAGTGCCAGGCAGTGGGTGCAGGACAGTAGTGCAGTGCACTGTGCATGAGCCGAAGCAGGGCGAGGCATCACCTCACCCGGGAAGCACAAGGGGTCAGGGAATTCCCTTTCCTAGTCAAAGAAAAGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCGCCCTAATACTGCGCTCTTCCAACAAGCTTAACAAATGGCACACCAGGAGATTATATCCCATGCCTGGCTCAGAGGGTCCTACGCCCATGGAGCCTCGCTCATTGCTAGCACAGCAGTCTGAGGTCAAACTGCAAGGTGGCAGTGAGGCTGGGGGAGGGGTGCCCACCATTGTCCAGGCTTGAGCAGGTAAACAAAGCCGCCTGGAAGCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTGCCTCTGTAGGCTCCACCTCTAGGGGCAGGGCACAGACAAACAAAAGACAACAAGAACCTCTGCAGACTTAAATGTCCCTGTCTGACAGCTTTGAAGAGAGTAGTGGTTCTCCCAGCACATAGCTTCAGATCTGAGAACAGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCCGAGTAGCCTAACTGGGAGGCATCCCCCAGTAGGGCGGACTGACACCTCACATGGCTGGTACTCCTCTAAGACAAAACTTCCAGAGGAATGATCAGGCAGCAGCATTTGCGGTTCACCAATATCCACTGTTCTGCAGCCACCGCTGCTGATACCCAGGAAAACAGCATCTGGAGTGGACCTCCAGTAAACTCCAACAGACCTGCAGCTGAGGGTCCTGACTGTTAGAAGGAAAACTAACAAACAGAAAGGACATCCACACCAAAAACCCATCTGTACATCACCATCATCAAAGACCAAAGGTAGATAAAACCATAAAGATGGGGAAAAAGCAGAGCAGAAAAACTGGACACTCTAAAAATGAGAGTGCCTCTCCTTCTCCAAAGTAACGCAGCTCCTCACCAGCAATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGATCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTTGAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAACCGATGAGATCAACTGGAAGAAAGGGTATCAATGACGGAAGATGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACGAACAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACATCTAATTGGTGTAGCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGCCCAAATTCACATTCAGGAAATACAGAGAACGCCACAAAGATACTCCTAGAGAAAAGCAACTCCAAGACACATAACTGACAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCTGATCTATCGGCAGAAACTCTACAAGCCAGAAGAAAGTGGGGGCCAATATTCAACATTGTTAAAGAAAAGAATTTTCGGCCCAGAATTTCATATCCAGCCAAACTAAGCTTCATAAGCATTGGAGAAATAAAATCCTTTACAGACAAGCAAATGCTGAGAGATTTTGTCACCACCAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACTAGTATCAGCCACTGCAAAAACATGCCAAATTGTAAACGACCATCAAGGCTAGGAAGAAACTGCATCAAGGAGCAAAATAACCAGCTAACATCATAATGACAGGATCAAATTCATACATAACAATACTCACCTTAAATGTAAATAGGCTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAAGGAGTCAAGACCCATCTGTCGTTATGTATTCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCGAAATAAAAGGATGGAGGAATATCTACCAAGCAAATGGAAAACAAAAAAAGGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGATTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAATACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCCCACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACACCACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAATGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGAACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGGGAAAGCAGGAAAGATCTAAAATTGACACCCTAACATCACAATTAAAAAACTAGAGAAGCAGGAGCAAACACATTCAAAAGCTAACAGAAGACAAGAAATAACTAAGATCAGAGCAGAAGTGAAGAAGATAGAGACACAAAAAACCCTTCAAAAAAATCAATGAATCCAGAAGCTGTTTTTTTGAAAAGATCAACAAAATTGATAGACTGCTAGCAAGACTAATAAAGAAGAAAGGGGAGAAGAATCAAATAGACGCAATAAAAAATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGCTCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGATTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATCCTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTGATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGAATCCAGCAGCACATCAAAAAGCTTATCCTCCATGATCAAGTGGGCTTCATCCCTGCCATGCAAGGCTGGTTCAACATACGAAATCAATAAACATAATCCAGCATATAAACAGAACCAAAGACACAAACCATATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAAATTCAACAATGCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACATATCTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAGTGGACAAAAACTGGAAGCATTCCCTTTGAAAACTGGCACAAGGCAGGGATGCCCTCTCTCACCACTCCTATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAAGGGCATTCAATTAGGAAAAGAGGAAGGTGAAATTGTCCCTGTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATATAAAATCAGTGTGCAAAAATCACAAGTATTCCTATGCACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTTATGGGTAGGAAGAATCATATCGTGAAAATGGTCATACTGCCCAAGGTAATTTATAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTTCTTCACAGAACTGGAAAAAACTACTTTAAAGTTCATATGGAATCAAAAAAGAGCCCACATCACCAAGGCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAATGCTACGGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATCTAGACCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATATCTACAACTATCCGATCTTTGACAAACCTGAGAGAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAAGACTTAAACATTAGACCTAAAACCATAAAAACCCTAGAAAAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGACAAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTGAACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCAAAAAGTGGGCAAAGGATATGAACAGACACTTCTCAAAAGAAGACATTTATGTAATCAAAAAACACATGAAAAAATGCTCATCATCACTAGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAACAGGAACAACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGCGGAAGTCAGTGTGGCAATTCCTCAGGAATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTAGATACCCAAAGGATTATAAATCATGCTGCTATAAAGACACATGCACACGTATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGGAACCAACCCAAATGTCCAACAACGATAGATTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACTATCATTCTCAGCAAACTATCACAAGGACAATAAACCAAACACCGCATGTTCTCACTCATAGGTGGGAATTGAACAATGAGAACACATGGACACATGAAGAGGAACATCACACTCTGGGGACTGTTATGGGGTGGGGGGCAGGGGCAGGGATAGCACTAGGAGATATACCTAATGCTAAATGACGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATATAACAAACCTGCCGTTGTGCACATGTACCCTAAAACTTGAAGTATAATAATAAAAAAAA primer3-2.3.6/test/primer_rat_output0000664000077300007730000212452012231156245016552 0ustar ioanaioanaPRIMER_OPT_SIZE=20 PRIMER_MAX_POLY_X=100 PRIMER_INTERNAL_MAX_POLY_X=100 PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000 PRIMER_MAX_GC=80.0 PRIMER_MAX_SIZE=25 PRIMER_MAX_TM=63.0 PRIMER_MIN_SIZE=18 PRIMER_MIN_GC=20 PRIMER_MIN_TM=57.0 PRIMER_OPT_TM=60.0 PRIMER_MAX_SELF_ANY=12 PRIMER_MAX_SELF_END=6 PRIMER_PAIR_MAX_COMPL_ANY=12 PRIMER_PAIR_MAX_COMPL_END=6 SEQUENCE_ID=01.RHAP33FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,315 SEQUENCE_TARGET=81,30 P3_COMMENT=CL is GCCTTCATTAGAAAGTGAAAGTAAA P3_COMMENT=CR is GGCAAAAGGACAGAGGATGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=7.733854 PRIMER_LEFT_0_PENALTY=7.538496 PRIMER_RIGHT_0_PENALTY=0.195358 PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_0_SEQUENCE=GGCAAAAGGACAGAGGATGA PRIMER_LEFT_0=20,25 PRIMER_RIGHT_0=177,20 PRIMER_LEFT_0_TM=57.462 PRIMER_RIGHT_0_TM=60.195 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.0000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=158 PRIMER_PAIR_1_PENALTY=7.868046 PRIMER_LEFT_1_PENALTY=7.538496 PRIMER_RIGHT_1_PENALTY=0.329550 PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_1_SEQUENCE=ATCTTGGGCAAAAGGACAGA PRIMER_LEFT_1=20,25 PRIMER_RIGHT_1=183,20 PRIMER_LEFT_1_TM=57.462 PRIMER_RIGHT_1_TM=59.670 PRIMER_LEFT_1_GC_PERCENT=32.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.0000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=164 PRIMER_PAIR_2_PENALTY=7.904946 PRIMER_LEFT_2_PENALTY=7.538496 PRIMER_RIGHT_2_PENALTY=0.366450 PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_2_SEQUENCE=TCTTGGGCAAAAGGACAGAG PRIMER_LEFT_2=20,25 PRIMER_RIGHT_2=182,20 PRIMER_LEFT_2_TM=57.462 PRIMER_RIGHT_2_TM=60.366 PRIMER_LEFT_2_GC_PERCENT=32.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.0000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=163 PRIMER_PAIR_3_PENALTY=8.845017 PRIMER_LEFT_3_PENALTY=7.538496 PRIMER_RIGHT_3_PENALTY=1.306521 PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_3_SEQUENCE=TATCTTGGGCAAAAGGACAGA PRIMER_LEFT_3=20,25 PRIMER_RIGHT_3=184,21 PRIMER_LEFT_3_TM=57.462 PRIMER_RIGHT_3_TM=59.693 PRIMER_LEFT_3_GC_PERCENT=32.000 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.0000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=165 PRIMER_PAIR_4_PENALTY=9.153890 PRIMER_LEFT_4_PENALTY=7.538496 PRIMER_RIGHT_4_PENALTY=1.615394 PRIMER_LEFT_4_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA PRIMER_RIGHT_4_SEQUENCE=ATCTTGGGCAAAAGGACAGAG PRIMER_LEFT_4=20,25 PRIMER_RIGHT_4=183,21 PRIMER_LEFT_4_TM=57.462 PRIMER_RIGHT_4_TM=60.615 PRIMER_LEFT_4_GC_PERCENT=32.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.0000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=164 = SEQUENCE_ID=01.RHAP34FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT SEQUENCE_INCLUDED_REGION=19,296 SEQUENCE_TARGET=134,15 SEQUENCE_TARGET=213,16 SEQUENCE_TARGET=235,16 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=03.RHAP34FA3.seq SEQUENCE_TEMPLATE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT SEQUENCE_INCLUDED_REGION=0,278 SEQUENCE_TARGET=1,28 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=03.RHAP34FE3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA SEQUENCE_INCLUDED_REGION=17,284 SEQUENCE_TARGET=27,60 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=04.RHAP33FE4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC SEQUENCE_INCLUDED_REGION=18,367 SEQUENCE_TARGET=146,46 SEQUENCE_TARGET=199,22 SEQUENCE_TARGET=286,13 SEQUENCE_TARGET=300,15 P3_COMMENT=CL is TGAGAAGGGAGGAGTAAGTACTGG P3_COMMENT=CR is CCTATGCCTATACACCACACACA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=7.353468 PRIMER_LEFT_0_PENALTY=4.174047 PRIMER_RIGHT_0_PENALTY=3.179421 PRIMER_LEFT_0_SEQUENCE=TGAGAAGGGAGGAGTAAGTACTGG PRIMER_RIGHT_0_SEQUENCE=CCTATGCCTATACACCACACACA PRIMER_LEFT_0=97,24 PRIMER_RIGHT_0=270,23 PRIMER_LEFT_0_TM=60.174 PRIMER_RIGHT_0_TM=59.821 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=47.826 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 PRIMER_PAIR_1_PENALTY=7.844268 PRIMER_LEFT_1_PENALTY=4.664847 PRIMER_RIGHT_1_PENALTY=3.179421 PRIMER_LEFT_1_SEQUENCE=GAAGGGAGGAGTAAGTACTGGAGA PRIMER_RIGHT_1_SEQUENCE=CCTATGCCTATACACCACACACA PRIMER_LEFT_1=100,24 PRIMER_RIGHT_1=270,23 PRIMER_LEFT_1_TM=59.335 PRIMER_RIGHT_1_TM=59.821 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=47.826 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=171 PRIMER_PAIR_2_PENALTY=7.844268 PRIMER_LEFT_2_PENALTY=4.664847 PRIMER_RIGHT_2_PENALTY=3.179421 PRIMER_LEFT_2_SEQUENCE=GAGAAGGGAGGAGTAAGTACTGGA PRIMER_RIGHT_2_SEQUENCE=CCTATGCCTATACACCACACACA PRIMER_LEFT_2=98,24 PRIMER_RIGHT_2=270,23 PRIMER_LEFT_2_TM=59.335 PRIMER_RIGHT_2_TM=59.821 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=47.826 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=173 PRIMER_PAIR_3_PENALTY=8.129938 PRIMER_LEFT_3_PENALTY=4.950517 PRIMER_RIGHT_3_PENALTY=3.179421 PRIMER_LEFT_3_SEQUENCE=GGGAGGAGTAAGTACTGGAGAGA PRIMER_RIGHT_3_SEQUENCE=CCTATGCCTATACACCACACACA PRIMER_LEFT_3=103,23 PRIMER_RIGHT_3=270,23 PRIMER_LEFT_3_TM=58.049 PRIMER_RIGHT_3_TM=59.821 PRIMER_LEFT_3_GC_PERCENT=52.174 PRIMER_RIGHT_3_GC_PERCENT=47.826 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=168 PRIMER_PAIR_4_PENALTY=8.185611 PRIMER_LEFT_4_PENALTY=5.006190 PRIMER_RIGHT_4_PENALTY=3.179421 PRIMER_LEFT_4_SEQUENCE=GGGAGGAGTAAGTACTGGAGAGAG PRIMER_RIGHT_4_SEQUENCE=CCTATGCCTATACACCACACACA PRIMER_LEFT_4=103,24 PRIMER_RIGHT_4=270,23 PRIMER_LEFT_4_TM=58.994 PRIMER_RIGHT_4_TM=59.821 PRIMER_LEFT_4_GC_PERCENT=54.167 PRIMER_RIGHT_4_GC_PERCENT=47.826 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=168 = SEQUENCE_ID=05.RHAP33FA5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA SEQUENCE_INCLUDED_REGION=10,369 SEQUENCE_TARGET=30,44 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=05.RHAP33FE5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,306 SEQUENCE_TARGET=176,34 P3_COMMENT=CL is CATGTTCGTGCAAACACTCA P3_COMMENT=CR is AGACCCTGTCTCCAAAAGCA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.858517 PRIMER_LEFT_0_PENALTY=0.702456 PRIMER_RIGHT_0_PENALTY=0.156061 PRIMER_LEFT_0_SEQUENCE=CATGTTCGTGCAAACACTCA PRIMER_RIGHT_0_SEQUENCE=AGACCCTGTCTCCAAAAGCA PRIMER_LEFT_0=129,20 PRIMER_RIGHT_0=302,20 PRIMER_LEFT_0_TM=59.298 PRIMER_RIGHT_0_TM=59.844 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 PRIMER_PAIR_1_PENALTY=0.922007 PRIMER_LEFT_1_PENALTY=0.702456 PRIMER_RIGHT_1_PENALTY=0.219551 PRIMER_LEFT_1_SEQUENCE=CATGTTCGTGCAAACACTCA PRIMER_RIGHT_1_SEQUENCE=CCCTGTCTCCAAAAGCAAAA PRIMER_LEFT_1=129,20 PRIMER_RIGHT_1=299,20 PRIMER_LEFT_1_TM=59.298 PRIMER_RIGHT_1_TM=60.220 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=171 PRIMER_PAIR_2_PENALTY=0.934756 PRIMER_LEFT_2_PENALTY=0.702456 PRIMER_RIGHT_2_PENALTY=0.232299 PRIMER_LEFT_2_SEQUENCE=CATGTTCGTGCAAACACTCA PRIMER_RIGHT_2_SEQUENCE=GACCCTGTCTCCAAAAGCAA PRIMER_LEFT_2=129,20 PRIMER_RIGHT_2=301,20 PRIMER_LEFT_2_TM=59.298 PRIMER_RIGHT_2_TM=60.232 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=173 PRIMER_PAIR_3_PENALTY=0.991260 PRIMER_LEFT_3_PENALTY=0.702456 PRIMER_RIGHT_3_PENALTY=0.288804 PRIMER_LEFT_3_SEQUENCE=CATGTTCGTGCAAACACTCA PRIMER_RIGHT_3_SEQUENCE=ACCCTGTCTCCAAAAGCAAA PRIMER_LEFT_3=129,20 PRIMER_RIGHT_3=300,20 PRIMER_LEFT_3_TM=59.298 PRIMER_RIGHT_3_TM=59.711 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=8.8000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=172 PRIMER_PAIR_4_PENALTY=1.268733 PRIMER_LEFT_4_PENALTY=1.112672 PRIMER_RIGHT_4_PENALTY=0.156061 PRIMER_LEFT_4_SEQUENCE=TGTTCGTGCAAACACTCAAA PRIMER_RIGHT_4_SEQUENCE=AGACCCTGTCTCCAAAAGCA PRIMER_LEFT_4=131,20 PRIMER_RIGHT_4=302,20 PRIMER_LEFT_4_TM=58.887 PRIMER_RIGHT_4_TM=59.844 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=172 = SEQUENCE_ID=05.RHAP34FA5.seq SEQUENCE_TEMPLATE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA SEQUENCE_INCLUDED_REGION=9,297 SEQUENCE_TARGET=125,36 SEQUENCE_TARGET=162,24 SEQUENCE_TARGET=191,16 SEQUENCE_TARGET=214,14 P3_COMMENT=CL is GCGGCCGACATCTACTACTA P3_COMMENT=CR is TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=9.884342 PRIMER_LEFT_0_PENALTY=1.973952 PRIMER_RIGHT_0_PENALTY=7.910390 PRIMER_LEFT_0_SEQUENCE=GCGGCCGACATCTACTACTA PRIMER_RIGHT_0_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_0=103,20 PRIMER_RIGHT_0=268,25 PRIMER_LEFT_0_TM=58.026 PRIMER_RIGHT_0_TM=57.090 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=44.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=4.7000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=166 PRIMER_PAIR_1_PENALTY=10.012256 PRIMER_LEFT_1_PENALTY=2.101866 PRIMER_RIGHT_1_PENALTY=7.910390 PRIMER_LEFT_1_SEQUENCE=GCGGCCGACATCTACTACTAC PRIMER_RIGHT_1_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_1=103,21 PRIMER_RIGHT_1=268,25 PRIMER_LEFT_1_TM=58.898 PRIMER_RIGHT_1_TM=57.090 PRIMER_LEFT_1_GC_PERCENT=57.143 PRIMER_RIGHT_1_GC_PERCENT=44.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=5.1000 PRIMER_RIGHT_1_END_STABILITY=6.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=166 PRIMER_PAIR_2_PENALTY=10.079221 PRIMER_LEFT_2_PENALTY=2.168831 PRIMER_RIGHT_2_PENALTY=7.910390 PRIMER_LEFT_2_SEQUENCE=GCGGCCGACATCTACTACTACT PRIMER_RIGHT_2_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_2=103,22 PRIMER_RIGHT_2=268,25 PRIMER_LEFT_2_TM=59.831 PRIMER_RIGHT_2_TM=57.090 PRIMER_LEFT_2_GC_PERCENT=54.545 PRIMER_RIGHT_2_GC_PERCENT=44.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=5.4000 PRIMER_RIGHT_2_END_STABILITY=6.0000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=166 PRIMER_PAIR_3_PENALTY=10.514211 PRIMER_LEFT_3_PENALTY=2.603822 PRIMER_RIGHT_3_PENALTY=7.910390 PRIMER_LEFT_3_SEQUENCE=CGCGGCCGACATCTACTACT PRIMER_RIGHT_3_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_3=102,20 PRIMER_RIGHT_3=268,25 PRIMER_LEFT_3_TM=62.604 PRIMER_RIGHT_3_TM=57.090 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=44.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=5.4000 PRIMER_RIGHT_3_END_STABILITY=6.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=167 PRIMER_PAIR_4_PENALTY=10.656616 PRIMER_LEFT_4_PENALTY=2.746227 PRIMER_RIGHT_4_PENALTY=7.910390 PRIMER_LEFT_4_SEQUENCE=CGCGGCCGACATCTACTAC PRIMER_RIGHT_4_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA PRIMER_LEFT_4=102,19 PRIMER_RIGHT_4=268,25 PRIMER_LEFT_4_TM=61.746 PRIMER_RIGHT_4_TM=57.090 PRIMER_LEFT_4_GC_PERCENT=63.158 PRIMER_RIGHT_4_GC_PERCENT=44.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=5.1000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=167 = SEQUENCE_ID=06.RHAP34FE6.seq SEQUENCE_TEMPLATE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA SEQUENCE_INCLUDED_REGION=8,287 SEQUENCE_TARGET=61,68 P3_COMMENT=CL is CAACCCAAAAATACAGACGGA P3_COMMENT=CR is CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.096487 PRIMER_LEFT_0_PENALTY=1.153194 PRIMER_RIGHT_0_PENALTY=0.943292 PRIMER_LEFT_0_SEQUENCE=CAACCCAAAAATACAGACGGA PRIMER_RIGHT_0_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_0=9,21 PRIMER_RIGHT_0=148,20 PRIMER_LEFT_0_TM=59.847 PRIMER_RIGHT_0_TM=60.943 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=140 PRIMER_PAIR_1_PENALTY=2.623573 PRIMER_LEFT_1_PENALTY=1.153194 PRIMER_RIGHT_1_PENALTY=1.470379 PRIMER_LEFT_1_SEQUENCE=CAACCCAAAAATACAGACGGA PRIMER_RIGHT_1_SEQUENCE=CCCTGTGTTACCCGTCTCA PRIMER_LEFT_1=9,21 PRIMER_RIGHT_1=148,19 PRIMER_LEFT_1_TM=59.847 PRIMER_RIGHT_1_TM=59.530 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=140 PRIMER_PAIR_2_PENALTY=2.943089 PRIMER_LEFT_2_PENALTY=1.999797 PRIMER_RIGHT_2_PENALTY=0.943292 PRIMER_LEFT_2_SEQUENCE=CAACCCAAAAATACAGACGG PRIMER_RIGHT_2_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_2=9,20 PRIMER_RIGHT_2=148,20 PRIMER_LEFT_2_TM=58.000 PRIMER_RIGHT_2_TM=60.943 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.6000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=5.00 PRIMER_PAIR_2_PRODUCT_SIZE=140 PRIMER_PAIR_3_PENALTY=2.943089 PRIMER_LEFT_3_PENALTY=1.999797 PRIMER_RIGHT_3_PENALTY=0.943292 PRIMER_LEFT_3_SEQUENCE=CCAACCCAAAAATACAGACG PRIMER_RIGHT_3_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_3=8,20 PRIMER_RIGHT_3=148,20 PRIMER_LEFT_3_TM=58.000 PRIMER_RIGHT_3_TM=60.943 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=141 PRIMER_PAIR_4_PENALTY=3.406904 PRIMER_LEFT_4_PENALTY=2.463612 PRIMER_RIGHT_4_PENALTY=0.943292 PRIMER_LEFT_4_SEQUENCE=CCAACCCAAAAATACAGACGG PRIMER_RIGHT_4_SEQUENCE=CCCTGTGTTACCCGTCTCAA PRIMER_LEFT_4=8,21 PRIMER_RIGHT_4=148,20 PRIMER_LEFT_4_TM=61.464 PRIMER_RIGHT_4_TM=60.943 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.6000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=5.00 PRIMER_PAIR_4_PRODUCT_SIZE=141 = SEQUENCE_ID=07.RHAP34FE7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT SEQUENCE_INCLUDED_REGION=18,283 SEQUENCE_TARGET=115,29 SEQUENCE_TARGET=145,20 SEQUENCE_TARGET=166,24 P3_COMMENT=CL is TGGGTACAAACTGACAGGCA P3_COMMENT=CR is TGTGCACGCATGTAGAGGTT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.484594 PRIMER_LEFT_0_PENALTY=0.151542 PRIMER_RIGHT_0_PENALTY=0.333053 PRIMER_LEFT_0_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_0_SEQUENCE=TGTGCACGCATGTAGAGGTT PRIMER_LEFT_0=76,20 PRIMER_RIGHT_0=238,20 PRIMER_LEFT_0_TM=60.152 PRIMER_RIGHT_0_TM=60.333 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=163 PRIMER_PAIR_1_PENALTY=0.681190 PRIMER_LEFT_1_PENALTY=0.151542 PRIMER_RIGHT_1_PENALTY=0.529649 PRIMER_LEFT_1_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_1_SEQUENCE=GCACGCATGTAGAGGTTCAG PRIMER_LEFT_1=76,20 PRIMER_RIGHT_1=235,20 PRIMER_LEFT_1_TM=60.152 PRIMER_RIGHT_1_TM=59.470 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=160 PRIMER_PAIR_2_PENALTY=0.789733 PRIMER_LEFT_2_PENALTY=0.151542 PRIMER_RIGHT_2_PENALTY=0.638191 PRIMER_LEFT_2_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_2_SEQUENCE=AGAGGTTCAGGTGGAGAGGG PRIMER_LEFT_2=76,20 PRIMER_RIGHT_2=225,20 PRIMER_LEFT_2_TM=60.152 PRIMER_RIGHT_2_TM=60.638 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=150 PRIMER_PAIR_3_PENALTY=0.828489 PRIMER_LEFT_3_PENALTY=0.151542 PRIMER_RIGHT_3_PENALTY=0.676947 PRIMER_LEFT_3_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_3_SEQUENCE=GTGCACGCATGTAGAGGTTC PRIMER_LEFT_3=76,20 PRIMER_RIGHT_3=237,20 PRIMER_LEFT_3_TM=60.152 PRIMER_RIGHT_3_TM=59.323 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=162 PRIMER_PAIR_4_PENALTY=0.837797 PRIMER_LEFT_4_PENALTY=0.151542 PRIMER_RIGHT_4_PENALTY=0.686256 PRIMER_LEFT_4_SEQUENCE=TGGGTACAAACTGACAGGCA PRIMER_RIGHT_4_SEQUENCE=CGCATGTAGAGGTTCAGGTG PRIMER_LEFT_4=76,20 PRIMER_RIGHT_4=232,20 PRIMER_LEFT_4_TM=60.152 PRIMER_RIGHT_4_TM=59.314 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=157 = SEQUENCE_ID=08.RHAP34FE8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG SEQUENCE_INCLUDED_REGION=20,281 SEQUENCE_TARGET=119,62 P3_COMMENT=CL is TTTCCCCTTAGGACCAAGGT P3_COMMENT=CR is CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.218990 PRIMER_LEFT_0_PENALTY=0.201280 PRIMER_RIGHT_0_PENALTY=0.017711 PRIMER_LEFT_0_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_0_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_0=53,20 PRIMER_RIGHT_0=220,20 PRIMER_LEFT_0_TM=59.799 PRIMER_RIGHT_0_TM=59.982 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=168 PRIMER_PAIR_1_PENALTY=0.267700 PRIMER_LEFT_1_PENALTY=0.249989 PRIMER_RIGHT_1_PENALTY=0.017711 PRIMER_LEFT_1_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_1_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_1=83,20 PRIMER_RIGHT_1=220,20 PRIMER_LEFT_1_TM=59.750 PRIMER_RIGHT_1_TM=59.982 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=138 PRIMER_PAIR_2_PENALTY=0.357340 PRIMER_LEFT_2_PENALTY=0.201280 PRIMER_RIGHT_2_PENALTY=0.156061 PRIMER_LEFT_2_SEQUENCE=TTTCCCCTTAGGACCAAGGT PRIMER_RIGHT_2_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_2=53,20 PRIMER_RIGHT_2=218,20 PRIMER_LEFT_2_TM=59.799 PRIMER_RIGHT_2_TM=59.844 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=166 PRIMER_PAIR_3_PENALTY=0.406050 PRIMER_LEFT_3_PENALTY=0.249989 PRIMER_RIGHT_3_PENALTY=0.156061 PRIMER_LEFT_3_SEQUENCE=GACCACGGATTATCCTTCCA PRIMER_RIGHT_3_SEQUENCE=GCTCTTCAAAGGACCTGGTG PRIMER_LEFT_3=83,20 PRIMER_RIGHT_3=218,20 PRIMER_LEFT_3_TM=59.750 PRIMER_RIGHT_3_TM=59.844 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=136 PRIMER_PAIR_4_PENALTY=0.440598 PRIMER_LEFT_4_PENALTY=0.422888 PRIMER_RIGHT_4_PENALTY=0.017711 PRIMER_LEFT_4_SEQUENCE=GCTTTTTCCCCTTAGGACCA PRIMER_RIGHT_4_SEQUENCE=CAGCTCTTCAAAGGACCTGG PRIMER_LEFT_4=49,20 PRIMER_RIGHT_4=220,20 PRIMER_LEFT_4_TM=60.423 PRIMER_RIGHT_4_TM=59.982 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=172 = SEQUENCE_ID=09.RHAP33FA9.seq SEQUENCE_TEMPLATE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG SEQUENCE_INCLUDED_REGION=0,371 SEQUENCE_TARGET=151,18 SEQUENCE_TARGET=192,53 SEQUENCE_TARGET=248,16 P3_COMMENT=CL is TGAGACATCCTGTTACCATGTAGG P3_COMMENT=CR is CTGTCCCTCCCTCCCTCC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=8.299756 PRIMER_LEFT_0_PENALTY=4.294975 PRIMER_RIGHT_0_PENALTY=4.004782 PRIMER_LEFT_0_SEQUENCE=TGAGACATCCTGTTACCATGTAGG PRIMER_RIGHT_0_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_0=19,24 PRIMER_RIGHT_0=191,18 PRIMER_LEFT_0_TM=60.295 PRIMER_RIGHT_0_TM=62.005 PRIMER_LEFT_0_GC_PERCENT=45.833 PRIMER_RIGHT_0_GC_PERCENT=72.222 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=1.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=173 PRIMER_PAIR_1_PENALTY=8.306042 PRIMER_LEFT_1_PENALTY=4.301260 PRIMER_RIGHT_1_PENALTY=4.004782 PRIMER_LEFT_1_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCCA PRIMER_RIGHT_1_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_1=67,24 PRIMER_RIGHT_1=191,18 PRIMER_LEFT_1_TM=60.301 PRIMER_RIGHT_1_TM=62.005 PRIMER_LEFT_1_GC_PERCENT=37.500 PRIMER_RIGHT_1_GC_PERCENT=72.222 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=125 PRIMER_PAIR_2_PENALTY=8.589410 PRIMER_LEFT_2_PENALTY=4.584629 PRIMER_RIGHT_2_PENALTY=4.004782 PRIMER_LEFT_2_SEQUENCE=GAGACATCCTGTTACCATGTAGGA PRIMER_RIGHT_2_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_2=20,24 PRIMER_RIGHT_2=191,18 PRIMER_LEFT_2_TM=59.415 PRIMER_RIGHT_2_TM=62.005 PRIMER_LEFT_2_GC_PERCENT=45.833 PRIMER_RIGHT_2_GC_PERCENT=72.222 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=172 PRIMER_PAIR_3_PENALTY=8.956691 PRIMER_LEFT_3_PENALTY=4.951910 PRIMER_RIGHT_3_PENALTY=4.004782 PRIMER_LEFT_3_SEQUENCE=TTGAGACATCCTGTTACCATGT PRIMER_RIGHT_3_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_3=18,22 PRIMER_RIGHT_3=191,18 PRIMER_LEFT_3_TM=57.048 PRIMER_RIGHT_3_TM=62.005 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=72.222 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=1.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=174 PRIMER_PAIR_4_PENALTY=9.135884 PRIMER_LEFT_4_PENALTY=5.131103 PRIMER_RIGHT_4_PENALTY=4.004782 PRIMER_LEFT_4_SEQUENCE=AGAGAGGTTTCATTTTCTCTGCT PRIMER_RIGHT_4_SEQUENCE=CTGTCCCTCCCTCCCTCC PRIMER_LEFT_4=48,23 PRIMER_RIGHT_4=191,18 PRIMER_LEFT_4_TM=57.869 PRIMER_RIGHT_4_TM=62.005 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=72.222 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=144 = SEQUENCE_ID=09.RHAP34FA9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT SEQUENCE_INCLUDED_REGION=20,280 SEQUENCE_TARGET=44,42 P3_COMMENT=CL is TGATCAGGATTTCAGTGCTAATAT P3_COMMENT=CR is TTCCTCACAGAGGGAACAGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=7.200927 PRIMER_LEFT_0_PENALTY=6.971746 PRIMER_RIGHT_0_PENALTY=0.229181 PRIMER_LEFT_0_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_0_SEQUENCE=TTCCTCACAGAGGGAACAGG PRIMER_LEFT_0=20,24 PRIMER_RIGHT_0=166,20 PRIMER_LEFT_0_TM=57.028 PRIMER_RIGHT_0_TM=60.229 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=5.8000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=147 PRIMER_PAIR_1_PENALTY=7.687872 PRIMER_LEFT_1_PENALTY=6.971746 PRIMER_RIGHT_1_PENALTY=0.716126 PRIMER_LEFT_1_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_1_SEQUENCE=CAGGGGCTGTTTCTGATTCT PRIMER_LEFT_1=20,24 PRIMER_RIGHT_1=150,20 PRIMER_LEFT_1_TM=57.028 PRIMER_RIGHT_1_TM=59.284 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=5.8000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=131 PRIMER_PAIR_2_PENALTY=7.830958 PRIMER_LEFT_2_PENALTY=6.971746 PRIMER_RIGHT_2_PENALTY=0.859212 PRIMER_LEFT_2_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_2_SEQUENCE=ACAGGGGCTGTTTCTGATTC PRIMER_LEFT_2=20,24 PRIMER_RIGHT_2=151,20 PRIMER_LEFT_2_TM=57.028 PRIMER_RIGHT_2_TM=59.141 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=5.8000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=132 PRIMER_PAIR_3_PENALTY=7.830958 PRIMER_LEFT_3_PENALTY=6.971746 PRIMER_RIGHT_3_PENALTY=0.859212 PRIMER_LEFT_3_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_3_SEQUENCE=GAACAGGGGCTGTTTCTGAT PRIMER_LEFT_3=20,24 PRIMER_RIGHT_3=153,20 PRIMER_LEFT_3_TM=57.028 PRIMER_RIGHT_3_TM=59.141 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=5.8000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=134 PRIMER_PAIR_4_PENALTY=8.039099 PRIMER_LEFT_4_PENALTY=6.971746 PRIMER_RIGHT_4_PENALTY=1.067353 PRIMER_LEFT_4_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT PRIMER_RIGHT_4_SEQUENCE=GGGCTGTTTCTGATTCTTTCC PRIMER_LEFT_4=20,24 PRIMER_RIGHT_4=147,21 PRIMER_LEFT_4_TM=57.028 PRIMER_RIGHT_4_TM=60.067 PRIMER_LEFT_4_GC_PERCENT=33.333 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=5.8000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=128 = SEQUENCE_ID=10.RHAP34FA10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=12,236 SEQUENCE_TARGET=155,32 P3_COMMENT=CL is CCTGCAATGGGATGAATACA P3_COMMENT=CR is CAAGGACAGAACTGAACTGGC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.750634 PRIMER_LEFT_0_PENALTY=0.649665 PRIMER_RIGHT_0_PENALTY=1.100969 PRIMER_LEFT_0_SEQUENCE=CCTGCAATGGGATGAATACA PRIMER_RIGHT_0_SEQUENCE=CAAGGACAGAACTGAACTGGC PRIMER_LEFT_0=101,20 PRIMER_RIGHT_0=229,21 PRIMER_LEFT_0_TM=59.350 PRIMER_RIGHT_0_TM=59.899 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.6000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=129 PRIMER_PAIR_1_PENALTY=1.750634 PRIMER_LEFT_1_PENALTY=0.649665 PRIMER_RIGHT_1_PENALTY=1.100969 PRIMER_LEFT_1_SEQUENCE=CCTGCAATGGGATGAATACA PRIMER_RIGHT_1_SEQUENCE=GCAAGGACAGAACTGAACTGG PRIMER_LEFT_1=101,20 PRIMER_RIGHT_1=230,21 PRIMER_LEFT_1_TM=59.350 PRIMER_RIGHT_1_TM=59.899 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=5.6000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=130 PRIMER_PAIR_2_PENALTY=2.364169 PRIMER_LEFT_2_PENALTY=1.263200 PRIMER_RIGHT_2_PENALTY=1.100969 PRIMER_LEFT_2_SEQUENCE=GATATCCTGCAATGGGATGAA PRIMER_RIGHT_2_SEQUENCE=CAAGGACAGAACTGAACTGGC PRIMER_LEFT_2=96,21 PRIMER_RIGHT_2=229,21 PRIMER_LEFT_2_TM=59.737 PRIMER_RIGHT_2_TM=59.899 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=134 PRIMER_PAIR_3_PENALTY=2.364169 PRIMER_LEFT_3_PENALTY=1.263200 PRIMER_RIGHT_3_PENALTY=1.100969 PRIMER_LEFT_3_SEQUENCE=GATATCCTGCAATGGGATGAA PRIMER_RIGHT_3_SEQUENCE=GCAAGGACAGAACTGAACTGG PRIMER_LEFT_3=96,21 PRIMER_RIGHT_3=230,21 PRIMER_LEFT_3_TM=59.737 PRIMER_RIGHT_3_TM=59.899 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=135 PRIMER_PAIR_4_PENALTY=2.578428 PRIMER_LEFT_4_PENALTY=0.649665 PRIMER_RIGHT_4_PENALTY=1.928763 PRIMER_LEFT_4_SEQUENCE=CCTGCAATGGGATGAATACA PRIMER_RIGHT_4_SEQUENCE=TGCAAGGACAGAACTGAACTG PRIMER_LEFT_4=101,20 PRIMER_RIGHT_4=231,21 PRIMER_LEFT_4_TM=59.350 PRIMER_RIGHT_4_TM=59.071 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=5.6000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=131 = SEQUENCE_ID=13.RHAP34FB1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,244 SEQUENCE_TARGET=22,16 SEQUENCE_TARGET=57,34 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=14.RHAP34FB2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC SEQUENCE_INCLUDED_REGION=13,303 SEQUENCE_TARGET=177,33 SEQUENCE_TARGET=254,14 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=14.RHAP34FF2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCATTTGGAAAGTCAACCTTCAAGGCGTGTGTTCTTCCTCCTTGGCACCTTGAGGAGGCAGCAATGACATTTTGACAAATGCCTCAAGTTAGGTAGGTGCTCTATAGCAAAGAGCCAATACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGTACGAATGTGTGTAATGAAAGGGGAATATTCTGGCTTAGCTTACACATTAAGTTCATTCAAGGTCATTCAATAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,248 SEQUENCE_TARGET=141,44 P3_COMMENT=CL is GGAGGCAGCAATGACATTTT P3_COMMENT=CR is TACACACATTCGTACACGCG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.855316 PRIMER_LEFT_0_PENALTY=0.080962 PRIMER_RIGHT_0_PENALTY=0.774354 PRIMER_LEFT_0_SEQUENCE=GGAGGCAGCAATGACATTTT PRIMER_RIGHT_0_SEQUENCE=TACACACATTCGTACACGCG PRIMER_LEFT_0=73,20 PRIMER_RIGHT_0=204,20 PRIMER_LEFT_0_TM=60.081 PRIMER_RIGHT_0_TM=59.226 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=11.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=132 PRIMER_PAIR_1_PENALTY=0.930642 PRIMER_LEFT_1_PENALTY=0.156287 PRIMER_RIGHT_1_PENALTY=0.774354 PRIMER_LEFT_1_SEQUENCE=AACCTTCAAGGCGTGTGTTC PRIMER_RIGHT_1_SEQUENCE=TACACACATTCGTACACGCG PRIMER_LEFT_1=34,20 PRIMER_RIGHT_1=204,20 PRIMER_LEFT_1_TM=60.156 PRIMER_RIGHT_1_TM=59.226 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=11.6000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=171 PRIMER_PAIR_2_PENALTY=1.005626 PRIMER_LEFT_2_PENALTY=0.231272 PRIMER_RIGHT_2_PENALTY=0.774354 PRIMER_LEFT_2_SEQUENCE=ACCTTCAAGGCGTGTGTTCT PRIMER_RIGHT_2_SEQUENCE=TACACACATTCGTACACGCG PRIMER_LEFT_2=35,20 PRIMER_RIGHT_2=204,20 PRIMER_LEFT_2_TM=59.769 PRIMER_RIGHT_2_TM=59.226 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=11.6000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=170 PRIMER_PAIR_3_PENALTY=1.005626 PRIMER_LEFT_3_PENALTY=0.231272 PRIMER_RIGHT_3_PENALTY=0.774354 PRIMER_LEFT_3_SEQUENCE=AGTCAACCTTCAAGGCGTGT PRIMER_RIGHT_3_SEQUENCE=TACACACATTCGTACACGCG PRIMER_LEFT_3=30,20 PRIMER_RIGHT_3=204,20 PRIMER_LEFT_3_TM=59.769 PRIMER_RIGHT_3_TM=59.226 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=11.6000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=5.00 PRIMER_PAIR_3_PRODUCT_SIZE=175 PRIMER_PAIR_4_PENALTY=1.064943 PRIMER_LEFT_4_PENALTY=0.290589 PRIMER_RIGHT_4_PENALTY=0.774354 PRIMER_LEFT_4_SEQUENCE=CCTTCAAGGCGTGTGTTCTT PRIMER_RIGHT_4_SEQUENCE=TACACACATTCGTACACGCG PRIMER_LEFT_4=36,20 PRIMER_RIGHT_4=204,20 PRIMER_LEFT_4_TM=60.291 PRIMER_RIGHT_4_TM=59.226 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=11.6000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=169 = SEQUENCE_ID=15.RHAP34FF3.seq SEQUENCE_TEMPLATE=NNNNNNNNCACTGCCTGTCTCTGCTCTTTACTGAGAACAGTCAAGGGGACAGTATTGTTATCTCCACATATTTCACACTTTTCTTAACCCTTTCCTTAAGATTTTATCTCCTCCATTTTNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGGCCCNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTGCTGGGCTCTTAAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=8,193 SEQUENCE_TARGET=119,24 SEQUENCE_TARGET=154,25 P3_COMMENT=CL is TGAGAACAGTCAAGGGGACA P3_COMMENT=CR is CCACTTAAGAGCCCAGCAAA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.148110 PRIMER_LEFT_0_PENALTY=0.770184 PRIMER_RIGHT_0_PENALTY=0.377925 PRIMER_LEFT_0_SEQUENCE=TGAGAACAGTCAAGGGGACA PRIMER_RIGHT_0_SEQUENCE=CCACTTAAGAGCCCAGCAAA PRIMER_LEFT_0=31,20 PRIMER_RIGHT_0=200,20 PRIMER_LEFT_0_TM=59.230 PRIMER_RIGHT_0_TM=60.378 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=170 PRIMER_PAIR_1_PENALTY=1.660265 PRIMER_LEFT_1_PENALTY=1.282340 PRIMER_RIGHT_1_PENALTY=0.377925 PRIMER_LEFT_1_SEQUENCE=TGAGAACAGTCAAGGGGACAG PRIMER_RIGHT_1_SEQUENCE=CCACTTAAGAGCCCAGCAAA PRIMER_LEFT_1=31,21 PRIMER_RIGHT_1=200,20 PRIMER_LEFT_1_TM=60.282 PRIMER_RIGHT_1_TM=60.378 PRIMER_LEFT_1_GC_PERCENT=52.381 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=8.8000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=170 PRIMER_PAIR_2_PENALTY=1.660265 PRIMER_LEFT_2_PENALTY=1.282340 PRIMER_RIGHT_2_PENALTY=0.377925 PRIMER_LEFT_2_SEQUENCE=CTGAGAACAGTCAAGGGGACA PRIMER_RIGHT_2_SEQUENCE=CCACTTAAGAGCCCAGCAAA PRIMER_LEFT_2=30,21 PRIMER_RIGHT_2=200,20 PRIMER_LEFT_2_TM=60.282 PRIMER_RIGHT_2_TM=60.378 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=8.8000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=171 PRIMER_PAIR_3_PENALTY=2.596154 PRIMER_LEFT_3_PENALTY=0.770184 PRIMER_RIGHT_3_PENALTY=1.825970 PRIMER_LEFT_3_SEQUENCE=TGAGAACAGTCAAGGGGACA PRIMER_RIGHT_3_SEQUENCE=CACTTAAGAGCCCAGCAAAA PRIMER_LEFT_3=31,20 PRIMER_RIGHT_3=199,20 PRIMER_LEFT_3_TM=59.230 PRIMER_RIGHT_3_TM=58.174 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=169 PRIMER_PAIR_4_PENALTY=2.606323 PRIMER_LEFT_4_PENALTY=0.770184 PRIMER_RIGHT_4_PENALTY=1.836139 PRIMER_LEFT_4_SEQUENCE=TGAGAACAGTCAAGGGGACA PRIMER_RIGHT_4_SEQUENCE=CACTTAAGAGCCCAGCAAAAG PRIMER_LEFT_4=31,20 PRIMER_RIGHT_4=199,21 PRIMER_LEFT_4_TM=59.230 PRIMER_RIGHT_4_TM=59.164 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=7.3000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=169 = SEQUENCE_ID=16.RHAP33FF4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCTTTGGACTCTGATGGAAGAACAGAATGAAAAGGATTATGGCCTCTAAGGGCAAAGGTACTCCACAGGCTTTGTCTTCATGTGGGTGTCGAACAACTAGAATTGGGCTTTCCCAAAACATGTTGTCTGTAGATGGGATATCTGTGTCTTTAAAATTCATTTATTTATTTACTCACTTTATATCCCAAAACTGGTGCTCCATCTTCATGGGATACCAACACCCATTGTTAGGGGAACCCTTAAAAAAACAAAGCGTGCGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGCAATGGGGGGTGAGGAGNCAGGAGGAGNNNNCNNAGGACAGAGGACCGAGT SEQUENCE_INCLUDED_REGION=17,363 SEQUENCE_TARGET=278,41 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=16.RHAP34FF4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCAGAAAACTCCGGAAGAGACCACCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAACCCATTCACGTGGGAGAGGGAGTGGACGGGCTCATTCTTGGATCTGCGGAGGGGTGGTTCGTAGGCACCTGGGAAGGGACTCTCCCATTTCTTACTGGTGTGGTGGGGTCTTGAAAGGAAGCTGCCCAAGCTGGGCGCTGGGCGGATGGCTCACGCCTTTAATTCCCCCGCACTGGGGGGGTAGCCAGTAGGAGAGATGGACCACCGANTTC SEQUENCE_INCLUDED_REGION=20,279 SEQUENCE_TARGET=46,38 P3_COMMENT=CL is AACTCCGGAAGAGACCACCT P3_COMMENT=CR is GAAATGGGAGAGTCCCTTCC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.236990 PRIMER_LEFT_0_PENALTY=0.110797 PRIMER_RIGHT_0_PENALTY=0.126193 PRIMER_LEFT_0_SEQUENCE=AACTCCGGAAGAGACCACCT PRIMER_RIGHT_0_SEQUENCE=GAAATGGGAGAGTCCCTTCC PRIMER_LEFT_0=25,20 PRIMER_RIGHT_0=177,20 PRIMER_LEFT_0_TM=60.111 PRIMER_RIGHT_0_TM=59.874 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=6.00 PRIMER_PAIR_0_PRODUCT_SIZE=153 PRIMER_PAIR_1_PENALTY=0.385991 PRIMER_LEFT_1_PENALTY=0.110797 PRIMER_RIGHT_1_PENALTY=0.275194 PRIMER_LEFT_1_SEQUENCE=AACTCCGGAAGAGACCACCT PRIMER_RIGHT_1_SEQUENCE=ACCCCACCACACCAGTAAGA PRIMER_LEFT_1=25,20 PRIMER_RIGHT_1=195,20 PRIMER_LEFT_1_TM=60.111 PRIMER_RIGHT_1_TM=60.275 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=171 PRIMER_PAIR_2_PENALTY=0.385991 PRIMER_LEFT_2_PENALTY=0.110797 PRIMER_RIGHT_2_PENALTY=0.275194 PRIMER_LEFT_2_SEQUENCE=AACTCCGGAAGAGACCACCT PRIMER_RIGHT_2_SEQUENCE=GACCCCACCACACCAGTAAG PRIMER_LEFT_2=25,20 PRIMER_RIGHT_2=196,20 PRIMER_LEFT_2_TM=60.111 PRIMER_RIGHT_2_TM=60.275 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=5.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=172 PRIMER_PAIR_3_PENALTY=0.385991 PRIMER_LEFT_3_PENALTY=0.110797 PRIMER_RIGHT_3_PENALTY=0.275194 PRIMER_LEFT_3_SEQUENCE=AACTCCGGAAGAGACCACCT PRIMER_RIGHT_3_SEQUENCE=AGACCCCACCACACCAGTAA PRIMER_LEFT_3=25,20 PRIMER_RIGHT_3=197,20 PRIMER_LEFT_3_TM=60.111 PRIMER_RIGHT_3_TM=60.275 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=5.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=173 PRIMER_PAIR_4_PENALTY=0.385991 PRIMER_LEFT_4_PENALTY=0.110797 PRIMER_RIGHT_4_PENALTY=0.275194 PRIMER_LEFT_4_SEQUENCE=AACTCCGGAAGAGACCACCT PRIMER_RIGHT_4_SEQUENCE=AAGACCCCACCACACCAGTA PRIMER_LEFT_4=25,20 PRIMER_RIGHT_4=198,20 PRIMER_LEFT_4_TM=60.111 PRIMER_RIGHT_4_TM=60.275 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=5.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=174 = SEQUENCE_ID=17.RHAP34FF5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCTTGACTTCTATGTATGCACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATCTTATACATCTCTATTGTGTCCTTGTCATGTGGAGGTCAGAGATGAATATACATCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGTTGTGGTGNACATCTCTCTCTGTTTATATTGTGGCTTGTCATGGGGAGGCCAAGAGGTTTAAGGACTCACACAGCTCT SEQUENCE_INCLUDED_REGION=18,285 SEQUENCE_TARGET=40,50 SEQUENCE_TARGET=154,26 SEQUENCE_TARGET=188,33 P3_COMMENT=CL is TCCTTGTCATGTGGAGGTCA P3_COMMENT=CR is CTTAAACCTCTTGGCCTCCC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.155493 PRIMER_LEFT_0_PENALTY=0.087977 PRIMER_RIGHT_0_PENALTY=0.067516 PRIMER_LEFT_0_SEQUENCE=TCCTTGTCATGTGGAGGTCA PRIMER_RIGHT_0_SEQUENCE=CTTAAACCTCTTGGCCTCCC PRIMER_LEFT_0=114,20 PRIMER_RIGHT_0=287,20 PRIMER_LEFT_0_TM=60.088 PRIMER_RIGHT_0_TM=60.068 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 PRIMER_PAIR_1_PENALTY=0.155493 PRIMER_LEFT_1_PENALTY=0.087977 PRIMER_RIGHT_1_PENALTY=0.067516 PRIMER_LEFT_1_SEQUENCE=TCCTTGTCATGTGGAGGTCA PRIMER_RIGHT_1_SEQUENCE=CCTTAAACCTCTTGGCCTCC PRIMER_LEFT_1=114,20 PRIMER_RIGHT_1=288,20 PRIMER_LEFT_1_TM=60.088 PRIMER_RIGHT_1_TM=60.068 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=6.00 PRIMER_PAIR_1_PRODUCT_SIZE=175 PRIMER_PAIR_2_PENALTY=0.970243 PRIMER_LEFT_2_PENALTY=0.902727 PRIMER_RIGHT_2_PENALTY=0.067516 PRIMER_LEFT_2_SEQUENCE=CCTTGTCATGTGGAGGTCAG PRIMER_RIGHT_2_SEQUENCE=CTTAAACCTCTTGGCCTCCC PRIMER_LEFT_2=115,20 PRIMER_RIGHT_2=287,20 PRIMER_LEFT_2_TM=59.097 PRIMER_RIGHT_2_TM=60.068 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=173 PRIMER_PAIR_3_PENALTY=0.970243 PRIMER_LEFT_3_PENALTY=0.902727 PRIMER_RIGHT_3_PENALTY=0.067516 PRIMER_LEFT_3_SEQUENCE=CCTTGTCATGTGGAGGTCAG PRIMER_RIGHT_3_SEQUENCE=CCTTAAACCTCTTGGCCTCC PRIMER_LEFT_3=115,20 PRIMER_RIGHT_3=288,20 PRIMER_LEFT_3_TM=59.097 PRIMER_RIGHT_3_TM=60.068 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=5.00 PRIMER_PAIR_3_PRODUCT_SIZE=174 PRIMER_PAIR_4_PENALTY=1.781024 PRIMER_LEFT_4_PENALTY=0.087977 PRIMER_RIGHT_4_PENALTY=1.693048 PRIMER_LEFT_4_SEQUENCE=TCCTTGTCATGTGGAGGTCA PRIMER_RIGHT_4_SEQUENCE=CCCCATGACAAGCCACAATA PRIMER_LEFT_4=114,20 PRIMER_RIGHT_4=270,20 PRIMER_LEFT_4_TM=60.088 PRIMER_RIGHT_4_TM=61.693 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=6.2000 PRIMER_PAIR_4_COMPL_ANY=10.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=157 = SEQUENCE_ID=18.RHAP34FB6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNACATGGTTATATGTCAAAATAATTTAATTTGTTGGTTGTTTCTTCCCAAAAATTACTTTAGTAAGAGTCCAAAAACTATTATTAATTAATATTAAGTTTTNGGNGNTGGTGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGTGATGTGTGCGTGATGTGAGCATGTTCCATGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=13,197 SEQUENCE_TARGET=127,46 P3_COMMENT=CL is TGTTGGTTGTTTCTTCCCAA P3_COMMENT=CR is CCATGGAACATGCTCACATC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.079588 PRIMER_LEFT_0_PENALTY=1.004672 PRIMER_RIGHT_0_PENALTY=0.074917 PRIMER_LEFT_0_SEQUENCE=TGTTGGTTGTTTCTTCCCAA PRIMER_RIGHT_0_SEQUENCE=CCATGGAACATGCTCACATC PRIMER_LEFT_0=42,20 PRIMER_RIGHT_0=208,20 PRIMER_LEFT_0_TM=58.995 PRIMER_RIGHT_0_TM=59.925 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=6.3000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=167 PRIMER_PAIR_1_PENALTY=1.079588 PRIMER_LEFT_1_PENALTY=1.004672 PRIMER_RIGHT_1_PENALTY=0.074917 PRIMER_LEFT_1_SEQUENCE=TTGTTGGTTGTTTCTTCCCA PRIMER_RIGHT_1_SEQUENCE=CCATGGAACATGCTCACATC PRIMER_LEFT_1=41,20 PRIMER_RIGHT_1=208,20 PRIMER_LEFT_1_TM=58.995 PRIMER_RIGHT_1_TM=59.925 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=6.3000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=168 PRIMER_PAIR_2_PENALTY=1.180452 PRIMER_LEFT_2_PENALTY=1.004672 PRIMER_RIGHT_2_PENALTY=0.175780 PRIMER_LEFT_2_SEQUENCE=TGTTGGTTGTTTCTTCCCAA PRIMER_RIGHT_2_SEQUENCE=TCACATCACGCACACATCAC PRIMER_LEFT_2=42,20 PRIMER_RIGHT_2=195,20 PRIMER_LEFT_2_TM=58.995 PRIMER_RIGHT_2_TM=60.176 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=10.0000 PRIMER_RIGHT_2_END_STABILITY=6.3000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=154 PRIMER_PAIR_3_PENALTY=1.180452 PRIMER_LEFT_3_PENALTY=1.004672 PRIMER_RIGHT_3_PENALTY=0.175780 PRIMER_LEFT_3_SEQUENCE=TTGTTGGTTGTTTCTTCCCA PRIMER_RIGHT_3_SEQUENCE=TCACATCACGCACACATCAC PRIMER_LEFT_3=41,20 PRIMER_RIGHT_3=195,20 PRIMER_LEFT_3_TM=58.995 PRIMER_RIGHT_3_TM=60.176 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.3000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=155 PRIMER_PAIR_4_PENALTY=1.336562 PRIMER_LEFT_4_PENALTY=1.004672 PRIMER_RIGHT_4_PENALTY=0.331890 PRIMER_LEFT_4_SEQUENCE=TGTTGGTTGTTTCTTCCCAA PRIMER_RIGHT_4_SEQUENCE=CTCACATCACGCACACATCA PRIMER_LEFT_4=42,20 PRIMER_RIGHT_4=196,20 PRIMER_LEFT_4_TM=58.995 PRIMER_RIGHT_4_TM=60.332 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=155 = SEQUENCE_ID=18.RHAP34FF6.seq SEQUENCE_TEMPLATE=NNNNNNNNNCCGTCTCAGTTAAAAGCAAATCTTGCCTCCATTCTGCGGGTTATTTTTGATGGTTTCCTCCTCATCTTCACTGGTCTGTTCTGGTGTTTGCTCCTAGGATTTGTCACGTGAATCCTGACTGATCCGACTTGCTTGTTTTTTTTTGCTCTTTCTTTAGTTCTCTATTTCTTTGCTCTTTTTCCTTGTACGGAAAGTTTTTTGCTTTCTCCTTTTTTCTTAAATGCACGCATGTGACTATTGTGATGGANNNNNNNNNNNNNNNNNNNNNNNNNATNNNN SEQUENCE_INCLUDED_REGION=9,274 SEQUENCE_TARGET=256,25 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=19.RHAP34FB7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTTTTCCACTTGTCTTGTTTCCAAACCTCCCTTTCCATCGAAAGCCATATAGTCATCTTTGTCCTATTATCAGCGNNNNNNNNNNNNNNNNNNNNANANANANGNGNGNGNGNGNANNNCNATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGGGGGNANAGAGNAATATGTGNATATGNGNANATANGGGGAAANACACANANANGGGGGNAGNGNGCGCGNAAGNGNNGNNAGAANCANGGGANNAGNAGAGTGNNNNGNGGGGGNAG SEQUENCE_INCLUDED_REGION=19,286 SEQUENCE_TARGET=93,20 SEQUENCE_TARGET=147,38 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=20.RHAP33FB8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTCTAGTAATCCTATTAGTTACACATGTAAATAACTAAGTGTAGCCCCTTAGGTGGAGACACCAGAGGGACAAGTGCATATTAGTTATTAGTAAACAAAAGAGCAAAAGGCTCTGAAGCTCTAGATCAACAAACAAAATCTATGGATGTAAAGAAATAATAAAGTCCAGGACAGAAGGAAATGAAACGTTGACAAAGTAATACCAATAATCAGTGAAACTAAAAGATGAGTGAGATTCACAGTTACTATTTGTAAGAGGTACATAGAATATTCGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATAGTGCCTTAAGTACATATATATTTTATAGAAATGNATTACTTTAGG SEQUENCE_INCLUDED_REGION=23,356 SEQUENCE_TARGET=299,30 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=20.RHAP34FB8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACAAAGTGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATACTGAGCTTCTAGAAGTAATATAGTTGCCAGAGANNANGGGGAAATATTGAGAACTTGCTAAATGTGTGCAGTCATAGTCAGACAAACAAAATAAGCTCTGGAGATCCATTACACAGATCGTGATTACAGCTCCCAATATTATTTCGAATAAGACACAGTAACTATACAAGGAAGCATTTGGATGTTCTTATACAGAAGAAATGATA SEQUENCE_INCLUDED_REGION=19,278 SEQUENCE_TARGET=30,56 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=20.RHAP34FF8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCAAATGCCAGCATGCAAAACTATAAAAGGATAAAAATGAAAATAGAAATGAAGTGTTGGCAGGAGCAGAAAGTGATGGCGCAGAATAGGAGGGCATTTCACAATAGTCCATGTGGCAAGAACTGTAGCCACTGATACATTTCTTTTTTAGTTCCTCTTTACAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCTTTAAAAATCTGCTTAAAAGAGCAGACAATATTAAGTTCCTG SEQUENCE_INCLUDED_REGION=20,279 SEQUENCE_TARGET=184,70 P3_COMMENT=CL is TGTGGCAAGAACTGTAGCCA P3_COMMENT=CR is TGTCTGCTCTTTTAAGCAGATTTTT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.475313 PRIMER_LEFT_0_PENALTY=0.449441 PRIMER_RIGHT_0_PENALTY=5.025871 PRIMER_LEFT_0_SEQUENCE=TGTGGCAAGAACTGTAGCCA PRIMER_RIGHT_0_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTTTT PRIMER_LEFT_0=130,20 PRIMER_RIGHT_0=284,25 PRIMER_LEFT_0_TM=60.449 PRIMER_RIGHT_0_TM=59.974 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=32.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=155 PRIMER_PAIR_1_PENALTY=5.475313 PRIMER_LEFT_1_PENALTY=0.449441 PRIMER_RIGHT_1_PENALTY=5.025871 PRIMER_LEFT_1_SEQUENCE=TGTGGCAAGAACTGTAGCCA PRIMER_RIGHT_1_SEQUENCE=TTGTCTGCTCTTTTAAGCAGATTTT PRIMER_LEFT_1=130,20 PRIMER_RIGHT_1=285,25 PRIMER_LEFT_1_TM=60.449 PRIMER_RIGHT_1_TM=59.974 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=32.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=7.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=156 PRIMER_PAIR_2_PENALTY=5.626457 PRIMER_LEFT_2_PENALTY=0.449441 PRIMER_RIGHT_2_PENALTY=5.177016 PRIMER_LEFT_2_SEQUENCE=TGTGGCAAGAACTGTAGCCA PRIMER_RIGHT_2_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTTT PRIMER_LEFT_2=130,20 PRIMER_RIGHT_2=284,24 PRIMER_LEFT_2_TM=60.449 PRIMER_RIGHT_2_TM=58.823 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=33.333 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=7.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=155 PRIMER_PAIR_3_PENALTY=5.626457 PRIMER_LEFT_3_PENALTY=0.449441 PRIMER_RIGHT_3_PENALTY=5.177016 PRIMER_LEFT_3_SEQUENCE=TGTGGCAAGAACTGTAGCCA PRIMER_RIGHT_3_SEQUENCE=TTGTCTGCTCTTTTAAGCAGATTT PRIMER_LEFT_3=130,20 PRIMER_RIGHT_3=285,24 PRIMER_LEFT_3_TM=60.449 PRIMER_RIGHT_3_TM=58.823 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=33.333 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_4_PENALTY=5.891375 PRIMER_LEFT_4_PENALTY=0.449441 PRIMER_RIGHT_4_PENALTY=5.441933 PRIMER_LEFT_4_SEQUENCE=TGTGGCAAGAACTGTAGCCA PRIMER_RIGHT_4_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTT PRIMER_LEFT_4=130,20 PRIMER_RIGHT_4=284,23 PRIMER_LEFT_4_TM=60.449 PRIMER_RIGHT_4_TM=57.558 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=34.783 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=155 = SEQUENCE_ID=21.RHAP33FB9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTCTTCATCCAGTCACCAACACGGGGCAGACTCAGGCTCATTGAGAAGCCTGCAGGTACAGAATGGCCCTGTTCCCTCTTACTAAATTTTGCTTGCAGTCAAGTTGCATAGCATGGAGTCCTGGTTGCAGGAATATCTCTATAGAGTTCTCAGATGCCCCCTCTCAGTGTTTCCATAAAACCTGGCTACCACGTGATGGAAGAAACAGCCTGATGTTACCATTTCCTTATTTTCACATGTATCTGCATGCATANNNNNNNNNNNNNNNNNNNNNNNNCTCTTGCTTGTAGAAGCTCACCAAAGCCATGCTTATACTACAGCCTGGTCTATTTCACATCCTTCAAGAGGTATACACAC SEQUENCE_INCLUDED_REGION=21,357 SEQUENCE_TARGET=274,24 P3_COMMENT=CL is AGTTCTCAGATGCCCCCTCT P3_COMMENT=CR is CATGGCTTTGGTGAGCTTCT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.613091 PRIMER_LEFT_0_PENALTY=0.216148 PRIMER_RIGHT_0_PENALTY=0.396944 PRIMER_LEFT_0_SEQUENCE=AGTTCTCAGATGCCCCCTCT PRIMER_RIGHT_0_SEQUENCE=CATGGCTTTGGTGAGCTTCT PRIMER_LEFT_0=165,20 PRIMER_RIGHT_0=328,20 PRIMER_LEFT_0_TM=60.216 PRIMER_RIGHT_0_TM=60.397 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=164 PRIMER_PAIR_1_PENALTY=0.613091 PRIMER_LEFT_1_PENALTY=0.216148 PRIMER_RIGHT_1_PENALTY=0.396944 PRIMER_LEFT_1_SEQUENCE=AGAGTTCTCAGATGCCCCCT PRIMER_RIGHT_1_SEQUENCE=CATGGCTTTGGTGAGCTTCT PRIMER_LEFT_1=163,20 PRIMER_RIGHT_1=328,20 PRIMER_LEFT_1_TM=60.216 PRIMER_RIGHT_1_TM=60.397 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=10.9000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=166 PRIMER_PAIR_2_PENALTY=0.919387 PRIMER_LEFT_2_PENALTY=0.522443 PRIMER_RIGHT_2_PENALTY=0.396944 PRIMER_LEFT_2_SEQUENCE=AAACCTGGCTACCACGTGAT PRIMER_RIGHT_2_SEQUENCE=CATGGCTTTGGTGAGCTTCT PRIMER_LEFT_2=198,20 PRIMER_RIGHT_2=328,20 PRIMER_LEFT_2_TM=59.478 PRIMER_RIGHT_2_TM=60.397 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.3000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=131 PRIMER_PAIR_3_PENALTY=0.947035 PRIMER_LEFT_3_PENALTY=0.550092 PRIMER_RIGHT_3_PENALTY=0.396944 PRIMER_LEFT_3_SEQUENCE=AAAACCTGGCTACCACGTGA PRIMER_RIGHT_3_SEQUENCE=CATGGCTTTGGTGAGCTTCT PRIMER_LEFT_3=197,20 PRIMER_RIGHT_3=328,20 PRIMER_LEFT_3_TM=60.550 PRIMER_RIGHT_3_TM=60.397 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=132 PRIMER_PAIR_4_PENALTY=1.158416 PRIMER_LEFT_4_PENALTY=0.761472 PRIMER_RIGHT_4_PENALTY=0.396944 PRIMER_LEFT_4_SEQUENCE=TAGAGTTCTCAGATGCCCCC PRIMER_RIGHT_4_SEQUENCE=CATGGCTTTGGTGAGCTTCT PRIMER_LEFT_4=162,20 PRIMER_RIGHT_4=328,20 PRIMER_LEFT_4_TM=59.239 PRIMER_RIGHT_4_TM=60.397 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=12.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=167 = SEQUENCE_ID=21.RHAP33FF9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTATGAAGTAGCAGTGAAACTAAGTTTATGGCTGGGAGTCATTAGAACATGAGGAACCGGTTAAAGGGTGACAGCGTTAGGACTGAGAACCTGTGCTTTAGAGCAAGCCTCAACTGCCCACTTGCAGACAGCAGTTCTCCAGTCATTTTCAACTTGTTCATTTCCAATGGAGACAAGATATCTATTTATCACGTGCAGCATGATAAATAANNNNNNNNNNNNNNNNNACATCCCCAACAGTGAGGACCAGGCTCTACTTGAAAACCCATTTCCACACCCACTCTCACTCATGAACAGACATACACATAATGAATGTATTTTTGTGAAGCACCCATTTCATACCTACCAAGAAAAATAATTCATTTTCT SEQUENCE_INCLUDED_REGION=27,367 SEQUENCE_TARGET=236,17 P3_COMMENT=CL is CCTGTGCTTTAGAGCAAGCC P3_COMMENT=CR is GTCCTCACTGTTGGGGATGT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.337261 PRIMER_LEFT_0_PENALTY=0.154023 PRIMER_RIGHT_0_PENALTY=0.183238 PRIMER_LEFT_0_SEQUENCE=CCTGTGCTTTAGAGCAAGCC PRIMER_RIGHT_0_SEQUENCE=GTCCTCACTGTTGGGGATGT PRIMER_LEFT_0=115,20 PRIMER_RIGHT_0=272,20 PRIMER_LEFT_0_TM=60.154 PRIMER_RIGHT_0_TM=59.817 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=6.3000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=158 PRIMER_PAIR_1_PENALTY=0.516560 PRIMER_LEFT_1_PENALTY=0.333322 PRIMER_RIGHT_1_PENALTY=0.183238 PRIMER_LEFT_1_SEQUENCE=TGCAGACAGCAGTTCTCCAG PRIMER_RIGHT_1_SEQUENCE=GTCCTCACTGTTGGGGATGT PRIMER_LEFT_1=148,20 PRIMER_RIGHT_1=272,20 PRIMER_LEFT_1_TM=60.333 PRIMER_RIGHT_1_TM=59.817 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.3000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=125 PRIMER_PAIR_2_PENALTY=0.561053 PRIMER_LEFT_2_PENALTY=0.377815 PRIMER_RIGHT_2_PENALTY=0.183238 PRIMER_LEFT_2_SEQUENCE=CCACTTGCAGACAGCAGTTC PRIMER_RIGHT_2_SEQUENCE=GTCCTCACTGTTGGGGATGT PRIMER_LEFT_2=143,20 PRIMER_RIGHT_2=272,20 PRIMER_LEFT_2_TM=59.622 PRIMER_RIGHT_2_TM=59.817 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.3000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=130 PRIMER_PAIR_3_PENALTY=0.626637 PRIMER_LEFT_3_PENALTY=0.333322 PRIMER_RIGHT_3_PENALTY=0.293316 PRIMER_LEFT_3_SEQUENCE=TGCAGACAGCAGTTCTCCAG PRIMER_RIGHT_3_SEQUENCE=TGAGTGAGAGTGGGTGTGGA PRIMER_LEFT_3=148,20 PRIMER_RIGHT_3=315,20 PRIMER_LEFT_3_TM=60.333 PRIMER_RIGHT_3_TM=60.293 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=1.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=168 PRIMER_PAIR_4_PENALTY=0.671131 PRIMER_LEFT_4_PENALTY=0.377815 PRIMER_RIGHT_4_PENALTY=0.293316 PRIMER_LEFT_4_SEQUENCE=CCACTTGCAGACAGCAGTTC PRIMER_RIGHT_4_SEQUENCE=TGAGTGAGAGTGGGTGTGGA PRIMER_LEFT_4=143,20 PRIMER_RIGHT_4=315,20 PRIMER_LEFT_4_TM=59.622 PRIMER_RIGHT_4_TM=60.293 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=173 = SEQUENCE_ID=21.RHAP34FB9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNGTAAAACATTCCTTCTTTCTAGCAATCATTCGTATACAAACTAAAACATAATCTGTGTCTTGCTTACAAGCATTTCAAATATTCAAAAATGTTATTTNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTAGTGTATGTCTATGTTTATATGTGTCCTTGTTTATATGTGTGTTCATGTTTATAGGTATGTGTCTATGTTTATATGTGTGTCCATGTTTATATGTGTGCCTGTTCATATGTGTGTTCATGNTTATAGGTGTGTGTCCATGTTTATAT SEQUENCE_INCLUDED_REGION=20,274 SEQUENCE_TARGET=117,26 P3_COMMENT=CL is TGTCTTGCTTACAAGCATTTCAA P3_COMMENT=CR is CAGGCACACATATAAACATGGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.622123 PRIMER_LEFT_0_PENALTY=3.061184 PRIMER_RIGHT_0_PENALTY=3.560938 PRIMER_LEFT_0_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA PRIMER_RIGHT_0_SEQUENCE=CAGGCACACATATAAACATGGA PRIMER_LEFT_0=75,23 PRIMER_RIGHT_0=248,22 PRIMER_LEFT_0_TM=59.939 PRIMER_RIGHT_0_TM=58.439 PRIMER_LEFT_0_GC_PERCENT=34.783 PRIMER_RIGHT_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 PRIMER_PAIR_1_PENALTY=6.767082 PRIMER_LEFT_1_PENALTY=3.061184 PRIMER_RIGHT_1_PENALTY=3.705897 PRIMER_LEFT_1_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA PRIMER_RIGHT_1_SEQUENCE=GGCACACATATAAACATGGACA PRIMER_LEFT_1=75,23 PRIMER_RIGHT_1=246,22 PRIMER_LEFT_1_TM=59.939 PRIMER_RIGHT_1_TM=58.294 PRIMER_LEFT_1_GC_PERCENT=34.783 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=172 PRIMER_PAIR_2_PENALTY=6.776871 PRIMER_LEFT_2_PENALTY=3.061184 PRIMER_RIGHT_2_PENALTY=3.715687 PRIMER_LEFT_2_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA PRIMER_RIGHT_2_SEQUENCE=AGGCACACATATAAACATGGACA PRIMER_LEFT_2=75,23 PRIMER_RIGHT_2=247,23 PRIMER_LEFT_2_TM=59.939 PRIMER_RIGHT_2_TM=59.284 PRIMER_LEFT_2_GC_PERCENT=34.783 PRIMER_RIGHT_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=173 PRIMER_PAIR_3_PENALTY=6.776871 PRIMER_LEFT_3_PENALTY=3.061184 PRIMER_RIGHT_3_PENALTY=3.715687 PRIMER_LEFT_3_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA PRIMER_RIGHT_3_SEQUENCE=CAGGCACACATATAAACATGGAC PRIMER_LEFT_3=75,23 PRIMER_RIGHT_3=248,23 PRIMER_LEFT_3_TM=59.939 PRIMER_RIGHT_3_TM=59.284 PRIMER_LEFT_3_GC_PERCENT=34.783 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=174 PRIMER_PAIR_4_PENALTY=6.776871 PRIMER_LEFT_4_PENALTY=3.061184 PRIMER_RIGHT_4_PENALTY=3.715687 PRIMER_LEFT_4_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA PRIMER_RIGHT_4_SEQUENCE=ACAGGCACACATATAAACATGGA PRIMER_LEFT_4=75,23 PRIMER_RIGHT_4=249,23 PRIMER_LEFT_4_TM=59.939 PRIMER_RIGHT_4_TM=59.284 PRIMER_LEFT_4_GC_PERCENT=34.783 PRIMER_RIGHT_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=175 = SEQUENCE_ID=22.RHAP33FF10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCTGCCTCCCAGGCAGGATGGCCGCTCACGTTGAGGGATGGCTCCCTACAGAGGCAGCAGGGGTAATGGGGAGCTTATCTTACTTCATTCACTCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGANNNNNNNNNNNNNNNNCTTATAATTTTGGGAGTTTTTTTCTTTTCATTATCTTTGGCCTCCAAAGATTTTAAAGAGTCNAGCCCAACTTCTTGCAGGTCTTCAGGGAACGCCCCAGTAAATCAAGCTGCAGGCAATGGACANGGAGAATACACCTTCCTCAAGCNCCCGAAAGAAGAGCCAAAAGGNAGAAGCCCACTGANNCCCGC SEQUENCE_INCLUDED_REGION=18,370 SEQUENCE_TARGET=113,63 SEQUENCE_TARGET=181,16 P3_COMMENT=CL is GGGGTAATGGGGAGCTTATC P3_COMMENT=CR is TTGGAGGCCAAAGATAATGAA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.839308 PRIMER_LEFT_0_PENALTY=0.372603 PRIMER_RIGHT_0_PENALTY=1.466705 PRIMER_LEFT_0_SEQUENCE=GGGGTAATGGGGAGCTTATC PRIMER_RIGHT_0_SEQUENCE=TGGAGGCCAAAGATAATGAAA PRIMER_LEFT_0=76,20 PRIMER_RIGHT_0=242,21 PRIMER_LEFT_0_TM=59.627 PRIMER_RIGHT_0_TM=59.533 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=5.9000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=167 PRIMER_PAIR_1_PENALTY=1.839308 PRIMER_LEFT_1_PENALTY=0.372603 PRIMER_RIGHT_1_PENALTY=1.466705 PRIMER_LEFT_1_SEQUENCE=GGGGTAATGGGGAGCTTATC PRIMER_RIGHT_1_SEQUENCE=TTGGAGGCCAAAGATAATGAA PRIMER_LEFT_1=76,20 PRIMER_RIGHT_1=243,21 PRIMER_LEFT_1_TM=59.627 PRIMER_RIGHT_1_TM=59.533 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=5.9000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=168 PRIMER_PAIR_2_PENALTY=1.839308 PRIMER_LEFT_2_PENALTY=0.372603 PRIMER_RIGHT_2_PENALTY=1.466705 PRIMER_LEFT_2_SEQUENCE=GGGGTAATGGGGAGCTTATC PRIMER_RIGHT_2_SEQUENCE=TTTGGAGGCCAAAGATAATGA PRIMER_LEFT_2=76,20 PRIMER_RIGHT_2=244,21 PRIMER_LEFT_2_TM=59.627 PRIMER_RIGHT_2_TM=59.533 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=5.9000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=169 PRIMER_PAIR_3_PENALTY=2.212475 PRIMER_LEFT_3_PENALTY=0.372603 PRIMER_RIGHT_3_PENALTY=1.839872 PRIMER_LEFT_3_SEQUENCE=GGGGTAATGGGGAGCTTATC PRIMER_RIGHT_3_SEQUENCE=TGGAGGCCAAAGATAATGAA PRIMER_LEFT_3=76,20 PRIMER_RIGHT_3=242,20 PRIMER_LEFT_3_TM=59.627 PRIMER_RIGHT_3_TM=58.160 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=5.9000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=167 PRIMER_PAIR_4_PENALTY=2.212475 PRIMER_LEFT_4_PENALTY=0.372603 PRIMER_RIGHT_4_PENALTY=1.839872 PRIMER_LEFT_4_SEQUENCE=GGGGTAATGGGGAGCTTATC PRIMER_RIGHT_4_SEQUENCE=TTGGAGGCCAAAGATAATGA PRIMER_LEFT_4=76,20 PRIMER_RIGHT_4=243,20 PRIMER_LEFT_4_TM=59.627 PRIMER_RIGHT_4_TM=58.160 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=5.9000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=168 = SEQUENCE_ID=22.RHAP34FB10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACACNCNCCCATGGTGAATGTGTTTCGNGCTCTTTTTCNTGGCTAATCAAGNGTTACCCCNTGGGGACATGAATCATGGAGAAACACAATACNAGAGAAANNTCCCAAGTTACCANNAGACCCAGTCCANGAATAGANGAGTAANNNNNNNNNNNNNNNNNAAAATATTGAAAANCCACTCACATACACAGNCATCNTACNTAACAAGNCACATACACATNNCACNGAGACACCACATGACACATACNCATAAATNAACAGACATAGAGAGAGACATAAAGACATGGACNCAAAGAGAANTATCATACACACAANGAAAGTAAAAAAATTACCACATTCAAACNCACNCANNGAGGACAANCAATTGNCAATTAAGAAAAGNCATACAAAAAATNCAAATNGAGANNGAT SEQUENCE_INCLUDED_REGION=23,438 SEQUENCE_TARGET=23,27 SEQUENCE_TARGET=195,17 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=22.RHAP34FF10.seq SEQUENCE_TEMPLATE=NNNNNNNNNCCTCATATCCGGCATACATGCCTTCCCTGCCATCCTCAGCTGTATCCCCACAAACCTGAACTAAAATAAACCCTTCCCTGTTGCTTTTCTTAGACATTCTGTCACAGCAGTGAGAAAGGTAACTAAAGCAGAATCCTGGTTCCAGAAGTGGGTTGTCCTTGTGATAAACCCATCTCATGGCGCCTAGGCTTTTGCAATTGGTTTATGTGAAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGTGATGGTGGGATGNT SEQUENCE_INCLUDED_REGION=9,284 SEQUENCE_TARGET=223,50 P3_COMMENT=CL is GCAGAATCCTGGTTCCAGAA P3_COMMENT=CR is CATCCCACCATCACAACC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.216911 PRIMER_LEFT_0_PENALTY=0.195358 PRIMER_RIGHT_0_PENALTY=4.021553 PRIMER_LEFT_0_SEQUENCE=GCAGAATCCTGGTTCCAGAA PRIMER_RIGHT_0_SEQUENCE=CATCCCACCATCACAACC PRIMER_LEFT_0=136,20 PRIMER_RIGHT_0=290,18 PRIMER_LEFT_0_TM=60.195 PRIMER_RIGHT_0_TM=57.978 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.556 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=155 PRIMER_PAIR_1_PENALTY=4.218456 PRIMER_LEFT_1_PENALTY=0.196903 PRIMER_RIGHT_1_PENALTY=4.021553 PRIMER_LEFT_1_SEQUENCE=AGCAGAATCCTGGTTCCAGA PRIMER_RIGHT_1_SEQUENCE=CATCCCACCATCACAACC PRIMER_LEFT_1=135,20 PRIMER_RIGHT_1=290,18 PRIMER_LEFT_1_TM=59.803 PRIMER_RIGHT_1_TM=57.978 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.556 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=156 PRIMER_PAIR_2_PENALTY=4.351103 PRIMER_LEFT_2_PENALTY=0.329550 PRIMER_RIGHT_2_PENALTY=4.021553 PRIMER_LEFT_2_SEQUENCE=AAAGCAGAATCCTGGTTCCA PRIMER_RIGHT_2_SEQUENCE=CATCCCACCATCACAACC PRIMER_LEFT_2=133,20 PRIMER_RIGHT_2=290,18 PRIMER_LEFT_2_TM=59.670 PRIMER_RIGHT_2_TM=57.978 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.556 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=158 PRIMER_PAIR_3_PENALTY=4.420878 PRIMER_LEFT_3_PENALTY=0.399325 PRIMER_RIGHT_3_PENALTY=4.021553 PRIMER_LEFT_3_SEQUENCE=TGGTTCCAGAAGTGGGTTGT PRIMER_RIGHT_3_SEQUENCE=CATCCCACCATCACAACC PRIMER_LEFT_3=145,20 PRIMER_RIGHT_3=290,18 PRIMER_LEFT_3_TM=60.399 PRIMER_RIGHT_3_TM=57.978 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.556 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=6.00 PRIMER_PAIR_3_PRODUCT_SIZE=146 PRIMER_PAIR_4_PENALTY=4.472396 PRIMER_LEFT_4_PENALTY=0.450843 PRIMER_RIGHT_4_PENALTY=4.021553 PRIMER_LEFT_4_SEQUENCE=TCCAGAAGTGGGTTGTCCTT PRIMER_RIGHT_4_SEQUENCE=CATCCCACCATCACAACC PRIMER_LEFT_4=149,20 PRIMER_RIGHT_4=290,18 PRIMER_LEFT_4_TM=59.549 PRIMER_RIGHT_4_TM=57.978 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.556 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=142 = SEQUENCE_ID=23.RHAP34FF11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCTACCAATTGTTCTTTATTTACAGGGAGGTCTACTCTGCCTCCTCATCCAGGCTTTCTTTCATTCATCTCCTTAGCTGATTGCATTAGATGCACACATTCATACACACAGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTGCTTCCCTGCAGAGGTCAAGTCTGGGAATAATACAAAGACTTCTCTGTAGGTCCACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,240 SEQUENCE_TARGET=133,64 P3_COMMENT=CL is CCTCCTCATCCAGGCTTTCT P3_COMMENT=CR is AGACTTGACCTCTGCAGGGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.739441 PRIMER_LEFT_0_PENALTY=0.725022 PRIMER_RIGHT_0_PENALTY=0.014419 PRIMER_LEFT_0_SEQUENCE=CCTCCTCATCCAGGCTTTCT PRIMER_RIGHT_0_SEQUENCE=AGACTTGACCTCTGCAGGGA PRIMER_LEFT_0=58,20 PRIMER_RIGHT_0=224,20 PRIMER_LEFT_0_TM=60.725 PRIMER_RIGHT_0_TM=59.986 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=167 PRIMER_PAIR_1_PENALTY=1.110778 PRIMER_LEFT_1_PENALTY=0.725022 PRIMER_RIGHT_1_PENALTY=0.385756 PRIMER_LEFT_1_SEQUENCE=CCTCCTCATCCAGGCTTTCT PRIMER_RIGHT_1_SEQUENCE=GACTTGACCTCTGCAGGGAA PRIMER_LEFT_1=58,20 PRIMER_RIGHT_1=223,20 PRIMER_LEFT_1_TM=60.725 PRIMER_RIGHT_1_TM=60.386 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=166 PRIMER_PAIR_2_PENALTY=1.110778 PRIMER_LEFT_2_PENALTY=0.725022 PRIMER_RIGHT_2_PENALTY=0.385756 PRIMER_LEFT_2_SEQUENCE=CCTCCTCATCCAGGCTTTCT PRIMER_RIGHT_2_SEQUENCE=TTCCCAGACTTGACCTCTGC PRIMER_LEFT_2=58,20 PRIMER_RIGHT_2=229,20 PRIMER_LEFT_2_TM=60.725 PRIMER_RIGHT_2_TM=60.386 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=172 PRIMER_PAIR_3_PENALTY=1.153193 PRIMER_LEFT_3_PENALTY=1.138774 PRIMER_RIGHT_3_PENALTY=0.014419 PRIMER_LEFT_3_SEQUENCE=CCTCATCCAGGCTTTCTTTC PRIMER_RIGHT_3_SEQUENCE=AGACTTGACCTCTGCAGGGA PRIMER_LEFT_3=61,20 PRIMER_RIGHT_3=224,20 PRIMER_LEFT_3_TM=58.861 PRIMER_RIGHT_3_TM=59.986 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=164 PRIMER_PAIR_4_PENALTY=1.153193 PRIMER_LEFT_4_PENALTY=1.138774 PRIMER_RIGHT_4_PENALTY=0.014419 PRIMER_LEFT_4_SEQUENCE=TCCTCATCCAGGCTTTCTTT PRIMER_RIGHT_4_SEQUENCE=AGACTTGACCTCTGCAGGGA PRIMER_LEFT_4=60,20 PRIMER_RIGHT_4=224,20 PRIMER_LEFT_4_TM=58.861 PRIMER_RIGHT_4_TM=59.986 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=165 = SEQUENCE_ID=24.RHAP33FB12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTTTTAGGTGTATAGGTTACATNAGCAAGGTTNCAGTTTAAACTTATTGGTTAGCATATTGACCTTTGAATCTGCTGGCTAGCAAGCATGGACAGATTATGACATGCCTTGGAACTCAATCTGGGACCAAAGGTTCAGGTGACTGTCAGCCAGTACTTNNNNNNNNNNNNNNNNNNNNNNNNNTNTNNNNNNNNNNNNNNTCTCTCNGCGTCNCNNCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTTTNTGTGTGTNTGTTTTTTTNNGNNTATGTNNGGCAGCNNTATNGGNATTTGGGNNTANCGCTTTNNGACNNAANNGNGNCCNNNAGTGNAC SEQUENCE_INCLUDED_REGION=23,356 SEQUENCE_TARGET=181,22 SEQUENCE_TARGET=209,14 SEQUENCE_TARGET=243,40 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=24.RHAP34FB12.seq SEQUENCE_TEMPLATE=NNNGGGAGNTAAGCATGGAATCAAATCTGACAATAACTAACTAACAATAGTTGNCCTACCAAGTTGNAACCAAGCAACCATTTTGCTGAAAAANTGCTNTCTTTTTTCCANTTGANNTGGTTTTGAGACNTCCNNTANGTCAAAAAAAAATCNAGTGAANCCANTANGAGANGNTAGANGAGAGATATCANTANTCNTAGAGAGNTACTATACAAATATACATANTAACAAAAATAAGAGATACATAATCATAGNTACATAGATACATACATAGNTAACACATAAAAATAGAAAATAGAAACAATATATAGAAAGAATATATAAATNNATATATATAGAGACACNNNNNNNNNNNNNNAAACAAATATNCNTAATAGATAGNAAAGACNGNCAGACAGACCCANNNCNNAACNNNCTNTCTAACTACAAAC SEQUENCE_INCLUDED_REGION=3,428 SEQUENCE_TARGET=344,14 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=26.RHAP33FC2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTCGGCACATTTTTAAAATAGATTCAATCATTCAAATTCAATCACAAATTTTCTACCCCATAATAATTTCTAATCATTTCACAGTGCACACACTCCTGCATCTAAATGCCTTCTTCCACATGTCAAAATAGAATTCATGTCTATTACCACACCTCTGAACTATCAAATCATTCAAAATGAAGATCATAAAATGAAGATGCATGGAAATGATGTGTAATGCCATAATAGGATAGCTACAAGAGTAACAGCAGGAGTAGCCAGTCCTTTGGAGAGTCATATTTAACCATGTGCTAAATAAGAGTTAGCAATATAATGCTGTAAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAC SEQUENCE_INCLUDED_REGION=23,361 SEQUENCE_TARGET=348,33 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=26.RHAP34FG2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTAGATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACCTGCTACTTAGAATCAGGAGCCACAGAACAGTGCAGTTCAGGAAACATCGGTTGACTTGATCATGAAGTCCTTAGAAGGGATGGAACCCACAGAACTGAAGATGTCCACAGAGAACNNTAGATAGACCATACTGAGGAACTCTGACATATCACTATCTACTGACATNTGATATATGAAGAGTATGACCACACAATCATATTATCATAGACNNTAATATATAGAAAAGNTTAGATNNCACAGAGATATATATCATGACTNTT SEQUENCE_INCLUDED_REGION=29,334 SEQUENCE_TARGET=43,54 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=27.RHAP33FC3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCTGCATTTTCTAGAAAGCATAACTAAAAGTACATGGGATCCTTGTGCGTGGCAGAGACATAATCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGAGAGAATANNNNNNNNNNNNNNNNNNNNAGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANAGAGCANNNNNNNNNNNNNNNAGACCTGANTGANNNNNNNNNNNNNNNNNNNNNNCCAANGCTGGACANAGTGANATCAGTNCCCCNNACATNAC SEQUENCE_INCLUDED_REGION=11,358 SEQUENCE_TARGET=77,58 SEQUENCE_TARGET=146,20 SEQUENCE_TARGET=171,38 SEQUENCE_TARGET=217,54 SEQUENCE_TARGET=281,14 SEQUENCE_TARGET=307,22 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=27.RHAP33FG3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNCTAGGCAATGGATGGACTGGCACGGAGGTCTAATGCACAGAGCCACACTGCTAGAGAAGTCTGACCTAAAAAGTCAGTCAAATTAATAAATGAGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGAGGGGCTATGGGAATCTAAGAAACACAGTTTAAAATTGCNTTTAAATTAAATTTTTAGTTAGTATACCAAATAGCATTTTATTATGACAGTTTTGTNTACNNNNNNNNNNNNNNNNNNNNNNNNCTATGTATGTATATATAATTGAANTTGGCCTNTTATTTGNCCCCCCANNNCCCCCCNCAAGGNATANA SEQUENCE_INCLUDED_REGION=16,365 SEQUENCE_TARGET=114,68 SEQUENCE_TARGET=289,24 P3_COMMENT=CL is GCACAGAGCCACACTGCTAG P3_COMMENT=CR is TAGATTCCCATAGCCCCTCC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.443596 PRIMER_LEFT_0_PENALTY=0.198986 PRIMER_RIGHT_0_PENALTY=0.244611 PRIMER_LEFT_0_SEQUENCE=GCACAGAGCCACACTGCTAG PRIMER_RIGHT_0_SEQUENCE=TAGATTCCCATAGCCCCTCC PRIMER_LEFT_0=50,20 PRIMER_RIGHT_0=206,20 PRIMER_LEFT_0_TM=59.801 PRIMER_RIGHT_0_TM=60.245 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=157 PRIMER_PAIR_1_PENALTY=0.930867 PRIMER_LEFT_1_PENALTY=0.686256 PRIMER_RIGHT_1_PENALTY=0.244611 PRIMER_LEFT_1_SEQUENCE=CACGGAGGTCTAATGCACAG PRIMER_RIGHT_1_SEQUENCE=TAGATTCCCATAGCCCCTCC PRIMER_LEFT_1=36,20 PRIMER_RIGHT_1=206,20 PRIMER_LEFT_1_TM=59.314 PRIMER_RIGHT_1_TM=60.245 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=171 PRIMER_PAIR_2_PENALTY=1.376889 PRIMER_LEFT_2_PENALTY=0.133741 PRIMER_RIGHT_2_PENALTY=1.243148 PRIMER_LEFT_2_SEQUENCE=ACTGGCACGGAGGTCTAATG PRIMER_RIGHT_2_SEQUENCE=AGATTCCCATAGCCCCTCC PRIMER_LEFT_2=31,20 PRIMER_RIGHT_2=205,19 PRIMER_LEFT_2_TM=60.134 PRIMER_RIGHT_2_TM=60.243 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.2000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=5.00 PRIMER_PAIR_2_PRODUCT_SIZE=175 PRIMER_PAIR_3_PENALTY=1.442134 PRIMER_LEFT_3_PENALTY=0.198986 PRIMER_RIGHT_3_PENALTY=1.243148 PRIMER_LEFT_3_SEQUENCE=GCACAGAGCCACACTGCTAG PRIMER_RIGHT_3_SEQUENCE=AGATTCCCATAGCCCCTCC PRIMER_LEFT_3=50,20 PRIMER_RIGHT_3=205,19 PRIMER_LEFT_3_TM=59.801 PRIMER_RIGHT_3_TM=60.243 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=57.895 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_4_PENALTY=1.656833 PRIMER_LEFT_4_PENALTY=1.412222 PRIMER_RIGHT_4_PENALTY=0.244611 PRIMER_LEFT_4_SEQUENCE=GAGGTCTAATGCACAGAGCCA PRIMER_RIGHT_4_SEQUENCE=TAGATTCCCATAGCCCCTCC PRIMER_LEFT_4=40,21 PRIMER_RIGHT_4=206,20 PRIMER_LEFT_4_TM=60.412 PRIMER_RIGHT_4_TM=60.245 PRIMER_LEFT_4_GC_PERCENT=52.381 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=167 = SEQUENCE_ID=28.RHAP33FC4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCTGTCCAACAGCCTGGCTGGATAGGTGAGGTCCAGCCTCAATATGAGATCCTGCCTCAAAAAATAAAGCATGATAGAGGAAGACACATAGACAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCTCAAAATTCTCTATTCCTTTAAACATATTCTCAGTCATCAGTAAAAGAAAATTTCTATTTATTAATAAGTTAGTAGGAATTATTTTGTTGCTAGTAGATTTTGATACAGACTTGTGTTGTCCAGGTTAGTCTTAAACTGGTCTCACATATTTTACTGCAGTCTGAAGTTACAGGCCTGTGTCANTGTGNNTGGCTTACAGNATTTGCT SEQUENCE_INCLUDED_REGION=22,359 SEQUENCE_TARGET=117,50 P3_COMMENT=CL is GGTGAGGTCCAGCCTCAATA P3_COMMENT=CR is GGCCTGTAACTTCAGACTGCA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.519602 PRIMER_LEFT_0_PENALTY=0.072951 PRIMER_RIGHT_0_PENALTY=1.446651 PRIMER_LEFT_0_SEQUENCE=GGTGAGGTCCAGCCTCAATA PRIMER_RIGHT_0_SEQUENCE=GGCCTGTAACTTCAGACTGCA PRIMER_LEFT_0=45,20 PRIMER_RIGHT_0=348,21 PRIMER_LEFT_0_TM=60.073 PRIMER_RIGHT_0_TM=60.447 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=6.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=304 PRIMER_PAIR_1_PENALTY=1.723417 PRIMER_LEFT_1_PENALTY=0.072951 PRIMER_RIGHT_1_PENALTY=1.650466 PRIMER_LEFT_1_SEQUENCE=GGTGAGGTCCAGCCTCAATA PRIMER_RIGHT_1_SEQUENCE=TGACACAGGCCTGTAACTTCA PRIMER_LEFT_1=45,20 PRIMER_RIGHT_1=355,21 PRIMER_LEFT_1_TM=60.073 PRIMER_RIGHT_1_TM=59.350 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.2000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=311 PRIMER_PAIR_2_PENALTY=1.767555 PRIMER_LEFT_2_PENALTY=0.320904 PRIMER_RIGHT_2_PENALTY=1.446651 PRIMER_LEFT_2_SEQUENCE=ATAGGTGAGGTCCAGCCTCA PRIMER_RIGHT_2_SEQUENCE=GGCCTGTAACTTCAGACTGCA PRIMER_LEFT_2=42,20 PRIMER_RIGHT_2=348,21 PRIMER_LEFT_2_TM=59.679 PRIMER_RIGHT_2_TM=60.447 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=307 PRIMER_PAIR_3_PENALTY=1.767555 PRIMER_LEFT_3_PENALTY=0.320904 PRIMER_RIGHT_3_PENALTY=1.446651 PRIMER_LEFT_3_SEQUENCE=CTGGATAGGTGAGGTCCAGC PRIMER_RIGHT_3_SEQUENCE=GGCCTGTAACTTCAGACTGCA PRIMER_LEFT_3=38,20 PRIMER_RIGHT_3=348,21 PRIMER_LEFT_3_TM=59.679 PRIMER_RIGHT_3_TM=60.447 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=311 PRIMER_PAIR_4_PENALTY=1.767555 PRIMER_LEFT_4_PENALTY=0.320904 PRIMER_RIGHT_4_PENALTY=1.446651 PRIMER_LEFT_4_SEQUENCE=GCTGGATAGGTGAGGTCCAG PRIMER_RIGHT_4_SEQUENCE=GGCCTGTAACTTCAGACTGCA PRIMER_LEFT_4=37,20 PRIMER_RIGHT_4=348,21 PRIMER_LEFT_4_TM=59.679 PRIMER_RIGHT_4_TM=60.447 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=312 = SEQUENCE_ID=28.RHAP34FC4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNACAATAATATATGAGTTGTTCCTTTAATCATGAATCATATTTCCTAGTATATTTTGAAACAATATTATTCATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATCTTCAAATGTACTGAATATTTCATCTATCACTTCTAATACTGAGGAAGCACCCAAGCACTCACCTTCAGAGAGGCAAAAGTGTTAGGGAAAGCACATTTCTTCTTCTTAAATACAT SEQUENCE_INCLUDED_REGION=12,274 SEQUENCE_TARGET=91,72 P3_COMMENT=CL is GAGTTGTTCCTTTAATCATGAATCA P3_COMMENT=CR is GCCTCTCTGAAGGTGAGTGC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.521283 PRIMER_LEFT_0_PENALTY=6.377880 PRIMER_RIGHT_0_PENALTY=0.143403 PRIMER_LEFT_0_SEQUENCE=GAGTTGTTCCTTTAATCATGAATCA PRIMER_RIGHT_0_SEQUENCE=GCCTCTCTGAAGGTGAGTGC PRIMER_LEFT_0=24,25 PRIMER_RIGHT_0=244,20 PRIMER_LEFT_0_TM=58.622 PRIMER_RIGHT_0_TM=60.143 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=221 PRIMER_PAIR_1_PENALTY=6.521283 PRIMER_LEFT_1_PENALTY=6.377880 PRIMER_RIGHT_1_PENALTY=0.143403 PRIMER_LEFT_1_SEQUENCE=TGAGTTGTTCCTTTAATCATGAATC PRIMER_RIGHT_1_SEQUENCE=GCCTCTCTGAAGGTGAGTGC PRIMER_LEFT_1=23,25 PRIMER_RIGHT_1=244,20 PRIMER_LEFT_1_TM=58.622 PRIMER_RIGHT_1_TM=60.143 PRIMER_LEFT_1_GC_PERCENT=32.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=222 PRIMER_PAIR_2_PENALTY=6.675898 PRIMER_LEFT_2_PENALTY=6.377880 PRIMER_RIGHT_2_PENALTY=0.298018 PRIMER_LEFT_2_SEQUENCE=GAGTTGTTCCTTTAATCATGAATCA PRIMER_RIGHT_2_SEQUENCE=CTGAAGGTGAGTGCTTGGGT PRIMER_LEFT_2=24,25 PRIMER_RIGHT_2=238,20 PRIMER_LEFT_2_TM=58.622 PRIMER_RIGHT_2_TM=60.298 PRIMER_LEFT_2_GC_PERCENT=32.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=215 PRIMER_PAIR_3_PENALTY=6.675898 PRIMER_LEFT_3_PENALTY=6.377880 PRIMER_RIGHT_3_PENALTY=0.298018 PRIMER_LEFT_3_SEQUENCE=TGAGTTGTTCCTTTAATCATGAATC PRIMER_RIGHT_3_SEQUENCE=CTGAAGGTGAGTGCTTGGGT PRIMER_LEFT_3=23,25 PRIMER_RIGHT_3=238,20 PRIMER_LEFT_3_TM=58.622 PRIMER_RIGHT_3_TM=60.298 PRIMER_LEFT_3_GC_PERCENT=32.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=216 PRIMER_PAIR_4_PENALTY=6.774824 PRIMER_LEFT_4_PENALTY=6.377880 PRIMER_RIGHT_4_PENALTY=0.396944 PRIMER_LEFT_4_SEQUENCE=GAGTTGTTCCTTTAATCATGAATCA PRIMER_RIGHT_4_SEQUENCE=GCTTGGGTGCTTCCTCAGTA PRIMER_LEFT_4=24,25 PRIMER_RIGHT_4=226,20 PRIMER_LEFT_4_TM=58.622 PRIMER_RIGHT_4_TM=60.397 PRIMER_LEFT_4_GC_PERCENT=32.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=5.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=5.00 PRIMER_PAIR_4_PRODUCT_SIZE=203 = SEQUENCE_ID=28.RHAP34FG4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCTGGTATCCTCCCTCTTTTGTCTTAGTTTAACCAGGAGAAACTCTCCCAAACTTTAACCTTATTAACAATTGCATGCAAAGTATGCCTGACTTAGGAATGTGGTGCAAAAGTCAGTCCCAAGGCGAGGAAATAACAGGTATTTTCTGAAGTCTTGTCTTAAGTCTGTTGTTGCAGCGGCCTTGAGTTTAATCCGTGGCACTGAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGGGGCAAACTGGAATCTCAGGAA SEQUENCE_INCLUDED_REGION=20,280 SEQUENCE_TARGET=233,42 P3_COMMENT=CL is CAAGGCGAGGAAATAACAGG P3_COMMENT=CR is TCCTGAGATTCCAGTTTGCC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.493482 PRIMER_LEFT_0_PENALTY=0.298125 PRIMER_RIGHT_0_PENALTY=0.195358 PRIMER_LEFT_0_SEQUENCE=CAAGGCGAGGAAATAACAGG PRIMER_RIGHT_0_SEQUENCE=TCCTGAGATTCCAGTTTGCC PRIMER_LEFT_0=139,20 PRIMER_RIGHT_0=298,20 PRIMER_LEFT_0_TM=59.702 PRIMER_RIGHT_0_TM=60.195 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=10.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=160 PRIMER_PAIR_1_PENALTY=0.493482 PRIMER_LEFT_1_PENALTY=0.298125 PRIMER_RIGHT_1_PENALTY=0.195358 PRIMER_LEFT_1_SEQUENCE=CCAAGGCGAGGAAATAACAG PRIMER_RIGHT_1_SEQUENCE=TCCTGAGATTCCAGTTTGCC PRIMER_LEFT_1=138,20 PRIMER_RIGHT_1=298,20 PRIMER_LEFT_1_TM=59.702 PRIMER_RIGHT_1_TM=60.195 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=161 PRIMER_PAIR_2_PENALTY=1.144071 PRIMER_LEFT_2_PENALTY=0.948713 PRIMER_RIGHT_2_PENALTY=0.195358 PRIMER_LEFT_2_SEQUENCE=AGTCCCAAGGCGAGGAAATA PRIMER_RIGHT_2_SEQUENCE=TCCTGAGATTCCAGTTTGCC PRIMER_LEFT_2=134,20 PRIMER_RIGHT_2=298,20 PRIMER_LEFT_2_TM=60.949 PRIMER_RIGHT_2_TM=60.195 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.2000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=165 PRIMER_PAIR_3_PENALTY=1.216668 PRIMER_LEFT_3_PENALTY=1.021310 PRIMER_RIGHT_3_PENALTY=0.195358 PRIMER_LEFT_3_SEQUENCE=GTCCCAAGGCGAGGAAATA PRIMER_RIGHT_3_SEQUENCE=TCCTGAGATTCCAGTTTGCC PRIMER_LEFT_3=135,19 PRIMER_RIGHT_3=298,20 PRIMER_LEFT_3_TM=60.021 PRIMER_RIGHT_3_TM=60.195 PRIMER_LEFT_3_GC_PERCENT=52.632 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.2000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=164 PRIMER_PAIR_4_PENALTY=1.399259 PRIMER_LEFT_4_PENALTY=1.203901 PRIMER_RIGHT_4_PENALTY=0.195358 PRIMER_LEFT_4_SEQUENCE=TGCAAAAGTCAGTCCCAAGG PRIMER_RIGHT_4_SEQUENCE=TCCTGAGATTCCAGTTTGCC PRIMER_LEFT_4=124,20 PRIMER_RIGHT_4=298,20 PRIMER_LEFT_4_TM=61.204 PRIMER_RIGHT_4_TM=60.195 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=10.0000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=175 = SEQUENCE_ID=29.RHAP33FG5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCTAATGCAGCATTGATTTCTGAAGNNNNNNNNNNNNNNATTTGGGCTTCTGATAATTGTTTGGTTTGCCTATTGCCTTAGAATTAGTGTCGATTACGACCAACAGGAAAACTTCAAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTTTTCNCACATGGACCTNTTTGTTTGAAAGTCCCTGCNGCTGCTTTGGACTGTGTGGATANNGTGCTCCACCTAGNCAGTTTGTGTGTATGGCATGNATATGCANCACACACACCGTGGTGCNTGGNTGNCCTGTGNAGGANAANAGCAGGGATNTTANATT SEQUENCE_INCLUDED_REGION=14,371 SEQUENCE_TARGET=38,14 SEQUENCE_TARGET=133,87 P3_COMMENT=CL is CGACCAACAGGAAAACTTCAA P3_COMMENT=CR is ATCCACACAGTCCAAAGCAG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.407334 PRIMER_LEFT_0_PENALTY=1.131978 PRIMER_RIGHT_0_PENALTY=1.275356 PRIMER_LEFT_0_SEQUENCE=CGACCAACAGGAAAACTTCAA PRIMER_RIGHT_0_SEQUENCE=ATCCACACAGTCCAAAGCAG PRIMER_LEFT_0=109,21 PRIMER_RIGHT_0=281,20 PRIMER_LEFT_0_TM=60.132 PRIMER_RIGHT_0_TM=58.725 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=173 PRIMER_PAIR_1_PENALTY=2.529067 PRIMER_LEFT_1_PENALTY=1.253711 PRIMER_RIGHT_1_PENALTY=1.275356 PRIMER_LEFT_1_SEQUENCE=CGACCAACAGGAAAACTTCA PRIMER_RIGHT_1_SEQUENCE=ATCCACACAGTCCAAAGCAG PRIMER_LEFT_1=109,20 PRIMER_RIGHT_1=281,20 PRIMER_LEFT_1_TM=58.746 PRIMER_RIGHT_1_TM=58.725 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=173 PRIMER_PAIR_2_PENALTY=2.588584 PRIMER_LEFT_2_PENALTY=1.131978 PRIMER_RIGHT_2_PENALTY=1.456605 PRIMER_LEFT_2_SEQUENCE=CGACCAACAGGAAAACTTCAA PRIMER_RIGHT_2_SEQUENCE=CCACACAGTCCAAAGCAGC PRIMER_LEFT_2=109,21 PRIMER_RIGHT_2=279,19 PRIMER_LEFT_2_TM=60.132 PRIMER_RIGHT_2_TM=60.457 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=171 PRIMER_PAIR_3_PENALTY=2.650983 PRIMER_LEFT_3_PENALTY=1.375627 PRIMER_RIGHT_3_PENALTY=1.275356 PRIMER_LEFT_3_SEQUENCE=ACGACCAACAGGAAAACTTCA PRIMER_RIGHT_3_SEQUENCE=ATCCACACAGTCCAAAGCAG PRIMER_LEFT_3=108,21 PRIMER_RIGHT_3=281,20 PRIMER_LEFT_3_TM=59.624 PRIMER_RIGHT_3_TM=58.725 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=174 PRIMER_PAIR_4_PENALTY=2.710317 PRIMER_LEFT_4_PENALTY=1.253711 PRIMER_RIGHT_4_PENALTY=1.456605 PRIMER_LEFT_4_SEQUENCE=CGACCAACAGGAAAACTTCA PRIMER_RIGHT_4_SEQUENCE=CCACACAGTCCAAAGCAGC PRIMER_LEFT_4=109,20 PRIMER_RIGHT_4=279,19 PRIMER_LEFT_4_TM=58.746 PRIMER_RIGHT_4_TM=60.457 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=57.895 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=171 = SEQUENCE_ID=30.RHAP34FC6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNACCCACACGGGCACATTCAGCCGCANANATGAGNCNCAGNCGCACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNACAGCAACTTCTCACCAAACNATCCAAAACCTACNGACTTGAGGGGCNGTCCTTGTCGTAGCACTAAANGCANANGGCAGCAGGTGGACACTGGTGGTGTGGGCACCTTGNGTNNGAGAGNCGGGGGNNANGAGGNCCNGGAGGGGNGNNACCGANGCNTCGGNAAGNTTCGNNAANNCANNGGNTCANNAGNCGNGGNNNNCCNNGGGGGGGGNGNA SEQUENCE_INCLUDED_REGION=12,314 SEQUENCE_TARGET=58,18 P3_COMMENT=CL is CCACACGGGCACATTCAG P3_COMMENT=CR is CACACCACCAGTGTCCACC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.172776 PRIMER_LEFT_0_PENALTY=4.227928 PRIMER_RIGHT_0_PENALTY=1.944848 PRIMER_LEFT_0_SEQUENCE=CCACACGGGCACATTCAG PRIMER_RIGHT_0_SEQUENCE=CACACCACCAGTGTCCACC PRIMER_LEFT_0=14,18 PRIMER_RIGHT_0=208,19 PRIMER_LEFT_0_TM=62.228 PRIMER_RIGHT_0_TM=60.945 PRIMER_LEFT_0_GC_PERCENT=61.111 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=195 PRIMER_PAIR_1_PENALTY=6.172776 PRIMER_LEFT_1_PENALTY=4.227928 PRIMER_RIGHT_1_PENALTY=1.944848 PRIMER_LEFT_1_SEQUENCE=CCACACGGGCACATTCAG PRIMER_RIGHT_1_SEQUENCE=CCACACCACCAGTGTCCAC PRIMER_LEFT_1=14,18 PRIMER_RIGHT_1=209,19 PRIMER_LEFT_1_TM=62.228 PRIMER_RIGHT_1_TM=60.945 PRIMER_LEFT_1_GC_PERCENT=61.111 PRIMER_RIGHT_1_GC_PERCENT=63.158 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=196 PRIMER_PAIR_2_PENALTY=6.203441 PRIMER_LEFT_2_PENALTY=4.227928 PRIMER_RIGHT_2_PENALTY=1.975514 PRIMER_LEFT_2_SEQUENCE=CCACACGGGCACATTCAG PRIMER_RIGHT_2_SEQUENCE=ACACCACCAGTGTCCACCTG PRIMER_LEFT_2=14,18 PRIMER_RIGHT_2=207,20 PRIMER_LEFT_2_TM=62.228 PRIMER_RIGHT_2_TM=61.976 PRIMER_LEFT_2_GC_PERCENT=61.111 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=194 PRIMER_PAIR_3_PENALTY=6.203441 PRIMER_LEFT_3_PENALTY=4.227928 PRIMER_RIGHT_3_PENALTY=1.975514 PRIMER_LEFT_3_SEQUENCE=CCACACGGGCACATTCAG PRIMER_RIGHT_3_SEQUENCE=CACACCACCAGTGTCCACCT PRIMER_LEFT_3=14,18 PRIMER_RIGHT_3=208,20 PRIMER_LEFT_3_TM=62.228 PRIMER_RIGHT_3_TM=61.976 PRIMER_LEFT_3_GC_PERCENT=61.111 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=195 PRIMER_PAIR_4_PENALTY=6.268774 PRIMER_LEFT_4_PENALTY=4.227928 PRIMER_RIGHT_4_PENALTY=2.040846 PRIMER_LEFT_4_SEQUENCE=CCACACGGGCACATTCAG PRIMER_RIGHT_4_SEQUENCE=CCACACCACCAGTGTCCA PRIMER_LEFT_4=14,18 PRIMER_RIGHT_4=209,18 PRIMER_LEFT_4_TM=62.228 PRIMER_RIGHT_4_TM=59.959 PRIMER_LEFT_4_GC_PERCENT=61.111 PRIMER_RIGHT_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=196 = SEQUENCE_ID=32.RHAP33FG8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTAGATGACAGGGTGCCCGCCTCCTGTGGGCGCAGGTGTGAGTCCTTGTAAGGCAACCTTGGGTTCCTGGCTGGACCTCAGAGGTTTCCCTCCATTGAGTGTGAGAGGGACTGTGGTAGTGGGGAAGGAACTGGTGGGCAGTGGAGGGTTCGCTCTTTAGACAGAGTACTACATACATAGAGAGTAAAGCAAAGAGCCGTAAAGAGGTGTGTTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACTTGTGTGGGTACACACATGCATGCAGACCTGANGCCNTCNNCAATTACNTNC SEQUENCE_INCLUDED_REGION=29,362 SEQUENCE_TARGET=248,35 SEQUENCE_TARGET=288,48 P3_COMMENT=CL is AAGCAAAGAGCCGTAAAGAGG P3_COMMENT=CR is AGGTCTGCATGCATGTGTGT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.223834 PRIMER_LEFT_0_PENALTY=1.028128 PRIMER_RIGHT_0_PENALTY=0.195706 PRIMER_LEFT_0_SEQUENCE=AAGCAAAGAGCCGTAAAGAGG PRIMER_RIGHT_0_SEQUENCE=AGGTCTGCATGCATGTGTGT PRIMER_LEFT_0=217,21 PRIMER_RIGHT_0=368,20 PRIMER_LEFT_0_TM=60.028 PRIMER_RIGHT_0_TM=60.196 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=152 PRIMER_PAIR_1_PENALTY=1.452864 PRIMER_LEFT_1_PENALTY=1.257158 PRIMER_RIGHT_1_PENALTY=0.195706 PRIMER_LEFT_1_SEQUENCE=AGCAAAGAGCCGTAAAGAGG PRIMER_RIGHT_1_SEQUENCE=AGGTCTGCATGCATGTGTGT PRIMER_LEFT_1=218,20 PRIMER_RIGHT_1=368,20 PRIMER_LEFT_1_TM=58.743 PRIMER_RIGHT_1_TM=60.196 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=151 PRIMER_PAIR_2_PENALTY=1.591782 PRIMER_LEFT_2_PENALTY=1.396076 PRIMER_RIGHT_2_PENALTY=0.195706 PRIMER_LEFT_2_SEQUENCE=GCAAAGAGCCGTAAAGAGGT PRIMER_RIGHT_2_SEQUENCE=AGGTCTGCATGCATGTGTGT PRIMER_LEFT_2=219,20 PRIMER_RIGHT_2=368,20 PRIMER_LEFT_2_TM=58.604 PRIMER_RIGHT_2_TM=60.196 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.6000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=150 PRIMER_PAIR_3_PENALTY=1.639290 PRIMER_LEFT_3_PENALTY=1.443584 PRIMER_RIGHT_3_PENALTY=0.195706 PRIMER_LEFT_3_SEQUENCE=AGCAAAGAGCCGTAAAGAGGT PRIMER_RIGHT_3_SEQUENCE=AGGTCTGCATGCATGTGTGT PRIMER_LEFT_3=218,21 PRIMER_RIGHT_3=368,20 PRIMER_LEFT_3_TM=59.556 PRIMER_RIGHT_3_TM=60.196 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=151 PRIMER_PAIR_4_PENALTY=1.900512 PRIMER_LEFT_4_PENALTY=1.704806 PRIMER_RIGHT_4_PENALTY=0.195706 PRIMER_LEFT_4_SEQUENCE=AGCCGTAAAGAGGTGTGTTTG PRIMER_RIGHT_4_SEQUENCE=AGGTCTGCATGCATGTGTGT PRIMER_LEFT_4=225,21 PRIMER_RIGHT_4=368,20 PRIMER_LEFT_4_TM=59.295 PRIMER_RIGHT_4_TM=60.196 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=144 = SEQUENCE_ID=33.RHAP33FG9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCTAGTCCAGTCAAATGGATGATCACTAGGTGCAAAGGGGGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGANAACAGCNTCCACCTAGAGCACNNNNNNNNNNNNNNACNAAGNAAACACATTTTCATTCCACACTTACATCATGCACACACATGCNTGCNGTGCATGNNNNNNNNNNNNNNNNCCCCCCCCANATAACNATAAAACCATACAGCAAACATNNTGTACAACCACCACNATNNNNNNNNNNNNNNTTTGTNTTCCGNTGGGCCAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=15,312 SEQUENCE_TARGET=59,58 SEQUENCE_TARGET=145,14 SEQUENCE_TARGET=220,16 SEQUENCE_TARGET=292,14 P3_COMMENT=CL is GATCACTAGGTGCAAAGGGG P3_COMMENT=CR is GCATGTGTGTGCATGATGTAA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.437392 PRIMER_LEFT_0_PENALTY=0.449829 PRIMER_RIGHT_0_PENALTY=1.987562 PRIMER_LEFT_0_SEQUENCE=GATCACTAGGTGCAAAGGGG PRIMER_RIGHT_0_SEQUENCE=GCATGTGTGTGCATGATGTAA PRIMER_LEFT_0=34,20 PRIMER_RIGHT_0=207,21 PRIMER_LEFT_0_TM=59.550 PRIMER_RIGHT_0_TM=59.012 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=10.9000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 PRIMER_PAIR_1_PENALTY=2.484366 PRIMER_LEFT_1_PENALTY=0.449829 PRIMER_RIGHT_1_PENALTY=2.034537 PRIMER_LEFT_1_SEQUENCE=GATCACTAGGTGCAAAGGGG PRIMER_RIGHT_1_SEQUENCE=TGTGCATGATGTAAGTGTGGAA PRIMER_LEFT_1=34,20 PRIMER_RIGHT_1=200,22 PRIMER_LEFT_1_TM=59.550 PRIMER_RIGHT_1_TM=60.035 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=10.9000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=167 PRIMER_PAIR_2_PENALTY=2.515032 PRIMER_LEFT_2_PENALTY=0.449829 PRIMER_RIGHT_2_PENALTY=2.065202 PRIMER_LEFT_2_SEQUENCE=GATCACTAGGTGCAAAGGGG PRIMER_RIGHT_2_SEQUENCE=GCATGTGTGTGCATGATGTAAG PRIMER_LEFT_2=34,20 PRIMER_RIGHT_2=207,22 PRIMER_LEFT_2_TM=59.550 PRIMER_RIGHT_2_TM=60.065 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=10.9000 PRIMER_RIGHT_2_END_STABILITY=5.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=174 PRIMER_PAIR_3_PENALTY=2.892209 PRIMER_LEFT_3_PENALTY=0.449829 PRIMER_RIGHT_3_PENALTY=2.442380 PRIMER_LEFT_3_SEQUENCE=GATCACTAGGTGCAAAGGGG PRIMER_RIGHT_3_SEQUENCE=TGTGCATGATGTAAGTGTGGA PRIMER_LEFT_3=34,20 PRIMER_RIGHT_3=200,21 PRIMER_LEFT_3_TM=59.550 PRIMER_RIGHT_3_TM=58.558 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=167 PRIMER_PAIR_4_PENALTY=2.944233 PRIMER_LEFT_4_PENALTY=0.449829 PRIMER_RIGHT_4_PENALTY=2.494404 PRIMER_LEFT_4_SEQUENCE=GATCACTAGGTGCAAAGGGG PRIMER_RIGHT_4_SEQUENCE=TGTGTGCATGATGTAAGTGTGG PRIMER_LEFT_4=34,20 PRIMER_RIGHT_4=202,22 PRIMER_LEFT_4_TM=59.550 PRIMER_RIGHT_4_TM=60.494 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.455 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=10.9000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=169 = SEQUENCE_ID=34.RHAP33FG10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCTATTTTGATCTCCATATGTTTTATTTCTATATCTATTCCTAATAGTTTTTANNNNNNNNNNNNNNNNGCATGCANNNNNNNNNNNNNNNNNNNNNNNNTCTGCANNNNNNNNNNNNNNNNNNNNNNNNNNTTGCTTAAAGATCAAGAAAGATGAGTTGTACCTGAGAAAACTTGCTAAGTCCCCCACTAGTAGTGGATCGACAATTAGACATTTTCCCATCAAGGACTGAAGCGTCTTTCTTGCGGACAGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=17,254 SEQUENCE_TARGET=69,16 SEQUENCE_TARGET=92,24 SEQUENCE_TARGET=122,26 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=35.RHAP33FG11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTAGAGACCACTGCGTCTCAGGAAATAAGGTGAAAAGAAACTGAGGAAGACAGCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCACTCACACAAGTTTTCTCAATTCCAGGCTATTTCCCCTTAGCGATAAGTGTAAGTTCATTCAAGAGCAGATGCTATTTTTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=27,174 SEQUENCE_TARGET=83,30 P3_COMMENT=CL is ACCACTGCGTCTCAGGAAAT P3_COMMENT=CR is GCTAAGGGGAAATAGCCTGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.327181 PRIMER_LEFT_0_PENALTY=0.272667 PRIMER_RIGHT_0_PENALTY=0.054514 PRIMER_LEFT_0_SEQUENCE=ACCACTGCGTCTCAGGAAAT PRIMER_RIGHT_0_SEQUENCE=GCTAAGGGGAAATAGCCTGG PRIMER_LEFT_0=32,20 PRIMER_RIGHT_0=158,20 PRIMER_LEFT_0_TM=59.727 PRIMER_RIGHT_0_TM=60.055 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=127 PRIMER_PAIR_1_PENALTY=0.456501 PRIMER_LEFT_1_PENALTY=0.272667 PRIMER_RIGHT_1_PENALTY=0.183834 PRIMER_LEFT_1_SEQUENCE=ACCACTGCGTCTCAGGAAAT PRIMER_RIGHT_1_SEQUENCE=TCGCTAAGGGGAAATAGCCT PRIMER_LEFT_1=32,20 PRIMER_RIGHT_1=160,20 PRIMER_LEFT_1_TM=59.727 PRIMER_RIGHT_1_TM=60.184 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=129 PRIMER_PAIR_2_PENALTY=0.662598 PRIMER_LEFT_2_PENALTY=0.608084 PRIMER_RIGHT_2_PENALTY=0.054514 PRIMER_LEFT_2_SEQUENCE=AGAGACCACTGCGTCTCAGG PRIMER_RIGHT_2_SEQUENCE=GCTAAGGGGAAATAGCCTGG PRIMER_LEFT_2=28,20 PRIMER_RIGHT_2=158,20 PRIMER_LEFT_2_TM=60.608 PRIMER_RIGHT_2_TM=60.055 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=9.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=131 PRIMER_PAIR_3_PENALTY=0.717627 PRIMER_LEFT_3_PENALTY=0.272667 PRIMER_RIGHT_3_PENALTY=0.444960 PRIMER_LEFT_3_SEQUENCE=ACCACTGCGTCTCAGGAAAT PRIMER_RIGHT_3_SEQUENCE=ATCGCTAAGGGGAAATAGCC PRIMER_LEFT_3=32,20 PRIMER_RIGHT_3=161,20 PRIMER_LEFT_3_TM=59.727 PRIMER_RIGHT_3_TM=59.555 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=8.7000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=130 PRIMER_PAIR_4_PENALTY=0.791919 PRIMER_LEFT_4_PENALTY=0.608084 PRIMER_RIGHT_4_PENALTY=0.183834 PRIMER_LEFT_4_SEQUENCE=AGAGACCACTGCGTCTCAGG PRIMER_RIGHT_4_SEQUENCE=TCGCTAAGGGGAAATAGCCT PRIMER_LEFT_4=28,20 PRIMER_RIGHT_4=160,20 PRIMER_LEFT_4_TM=60.608 PRIMER_RIGHT_4_TM=60.184 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=9.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=133 = SEQUENCE_ID=36.RHAP33FG12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCTTCTGTTTAGATTGTGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGGTTTCACAGGCTGGCTATTCATGCTTCATGGTCTTCAGTTCTGGGATAACAGATATGGCCACAAGTGGCTTACTTTAAAGTTTTGACATTAACAAACATTTTTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=17,174 SEQUENCE_TARGET=37,44 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=36.RHAP34FG12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCAGGTTATGCAAGGCTGGTTGGGTGCAGGGCTAAGAGATGCTGAAAAAAAAAGTGTATCTACAATTAGATTATTTCATTTCATTTGCTGTGGAGAATCTACAGGTGTGTGAGTGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTTTGAACTACTCTTGGGATAACATGTGGAAGAGTCTGGGTGTGGAAAGCTGTCATTCGGGAAAGAAAAATAAGAGCACTGTGATCCCAGGTACAAACATTTTAGAGGCAGATTTTAGGGNNNN SEQUENCE_INCLUDED_REGION=19,276 SEQUENCE_TARGET=137,36 P3_COMMENT=CL is GCAGGGCTAAGAGATGCTGA P3_COMMENT=CR is CACACCCAGACTCTTCCACA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.940817 PRIMER_LEFT_0_PENALTY=0.645726 PRIMER_RIGHT_0_PENALTY=0.295091 PRIMER_LEFT_0_SEQUENCE=GCAGGGCTAAGAGATGCTGA PRIMER_RIGHT_0_SEQUENCE=CACACCCAGACTCTTCCACA PRIMER_LEFT_0=44,20 PRIMER_RIGHT_0=218,20 PRIMER_LEFT_0_TM=60.646 PRIMER_RIGHT_0_TM=59.705 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=175 PRIMER_PAIR_1_PENALTY=1.299667 PRIMER_LEFT_1_PENALTY=0.396940 PRIMER_RIGHT_1_PENALTY=0.902727 PRIMER_LEFT_1_SEQUENCE=GTGCAGGGCTAAGAGATGCT PRIMER_RIGHT_1_SEQUENCE=CACCCAGACTCTTCCACATG PRIMER_LEFT_1=42,20 PRIMER_RIGHT_1=216,20 PRIMER_LEFT_1_TM=59.603 PRIMER_RIGHT_1_TM=59.097 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.1000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=175 PRIMER_PAIR_2_PENALTY=1.414540 PRIMER_LEFT_2_PENALTY=0.396940 PRIMER_RIGHT_2_PENALTY=1.017601 PRIMER_LEFT_2_SEQUENCE=GTGCAGGGCTAAGAGATGCT PRIMER_RIGHT_2_SEQUENCE=CACCCAGACTCTTCCACATGT PRIMER_LEFT_2=42,20 PRIMER_RIGHT_2=216,21 PRIMER_LEFT_2_TM=59.603 PRIMER_RIGHT_2_TM=60.018 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=175 PRIMER_PAIR_3_PENALTY=1.548453 PRIMER_LEFT_3_PENALTY=0.645726 PRIMER_RIGHT_3_PENALTY=0.902727 PRIMER_LEFT_3_SEQUENCE=GCAGGGCTAAGAGATGCTGA PRIMER_RIGHT_3_SEQUENCE=CACCCAGACTCTTCCACATG PRIMER_LEFT_3=44,20 PRIMER_RIGHT_3=216,20 PRIMER_LEFT_3_TM=60.646 PRIMER_RIGHT_3_TM=59.097 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=173 PRIMER_PAIR_4_PENALTY=1.663327 PRIMER_LEFT_4_PENALTY=0.645726 PRIMER_RIGHT_4_PENALTY=1.017601 PRIMER_LEFT_4_SEQUENCE=GCAGGGCTAAGAGATGCTGA PRIMER_RIGHT_4_SEQUENCE=CACCCAGACTCTTCCACATGT PRIMER_LEFT_4=44,20 PRIMER_RIGHT_4=216,21 PRIMER_LEFT_4_TM=60.646 PRIMER_RIGHT_4_TM=60.018 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=173 = SEQUENCE_ID=37.RHAP33FD1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNCTTCACTTTCATACATGGAAGTCACTATCTCAGAGCAGATACCCTGGTCCTCTGTCCCTTACAATCTCTCCGTGCTATCTTTCAGAGATGTTCCCTAAGCCTTAGGTGTAGGGGTTGTGTTGAAGATGCACCAAGGATAAGGATTCAGTCAGTTGTCTGCGTTTTTTTTTACCAGTTCTGAGTTTCTCTGATACCCTTTATCTATATGCAGCAGCTGGCAGCAGTATGTACTGTTGGCAAACTGATAAAATGTTCTGGATCTGTAATGAAAGACACACATATAGATAAGTATATANACATNNNNNNNNNNNNNNNNAAGNGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNCA SEQUENCE_INCLUDED_REGION=10,361 SEQUENCE_TARGET=310,16 SEQUENCE_TARGET=333,33 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=37.RHAP33FH1.seq SEQUENCE_TEMPLATE=NNNNTCCCGGTACGGGCGCGCCCNCCCCCGNNNNNNNNNNNNNNNNNNNNNTGTGGNGCGCGTTTGTCGGNTTTGNGTCCAGAGGCTGTTGTTAGGTATGGAGGGNGTGGAGGNGGAGGGTGTTCTTGGTGCCAGTGTGTGTNCGCNGCTGTTGGCGTTGNNNTTTGNGGGNGTNTGANGACCAANTGGCCNACGGTGCTGTGACATCAACCAGCTGGCAAAGNAAGNAGGNGCNAAGAGCGCAGCCTTCCCANGCCNNCACNGCNNNACCTCCTGCNNNTCTCCTAACCCCNNNCNCCCNCTTCNGCCTNCNCCTGNNCCTCCCTCAAGGCCGCCNNCCCNNTGTCCNNCNNCCCGCCCNNCNGGAANAGCCCCTCCACCCCCNNCNGCCNCACCNNCAATCCTCCNCNTNAACCCNCTGCCNCCT SEQUENCE_INCLUDED_REGION=4,423 SEQUENCE_TARGET=30,21 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=37.RHAP34FD1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNACTACAAGGNNNNNNNNNNNNNNNNNNNATTCATATATACAGCACACGCATACAAATNNNNNNNNNNNNNNNNNNNNAACAAACATACTTACTTACACACCACACAGACCCACTACGAACTCACACAAACATTCTACATAGACACATCACATACATACCATGCAGTNNNNNNNNNNNNNNNNNNCTCATACCACACCCAATACACACACACACGTCTACTCTCTACTTCGTCNACTATCGACNACTACACACACNACNNCATCTATCTACNGCATTTACTTTACATACAAAGTTATACAGTAGNAGTAGAGTGNACCGATGACTCNGA SEQUENCE_INCLUDED_REGION=18,328 SEQUENCE_TARGET=27,19 SEQUENCE_TARGET=75,20 SEQUENCE_TARGET=184,18 P3_COMMENT=CL is ACTTACACACCACACAGACCC P3_COMMENT=CR is GACGTGTGTGTGTGTATTGGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.820251 PRIMER_LEFT_0_PENALTY=3.605542 PRIMER_RIGHT_0_PENALTY=1.214710 PRIMER_LEFT_0_SEQUENCE=ACTTACACACCACACAGACCC PRIMER_RIGHT_0_SEQUENCE=GACGTGTGTGTGTGTATTGGG PRIMER_LEFT_0=108,21 PRIMER_RIGHT_0=233,21 PRIMER_LEFT_0_TM=57.394 PRIMER_RIGHT_0_TM=59.785 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.1000 PRIMER_RIGHT_0_END_STABILITY=10.0000 PRIMER_PAIR_0_COMPL_ANY=10.00 PRIMER_PAIR_0_COMPL_END=6.00 PRIMER_PAIR_0_PRODUCT_SIZE=126 PRIMER_PAIR_1_PENALTY=5.267892 PRIMER_LEFT_1_PENALTY=4.053183 PRIMER_RIGHT_1_PENALTY=1.214710 PRIMER_LEFT_1_SEQUENCE=TTACTTACACACCACACAGACCC PRIMER_RIGHT_1_SEQUENCE=GACGTGTGTGTGTGTATTGGG PRIMER_LEFT_1=106,23 PRIMER_RIGHT_1=233,21 PRIMER_LEFT_1_TM=58.947 PRIMER_RIGHT_1_TM=59.785 PRIMER_LEFT_1_GC_PERCENT=47.826 PRIMER_RIGHT_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.1000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=10.00 PRIMER_PAIR_1_COMPL_END=6.00 PRIMER_PAIR_1_PRODUCT_SIZE=128 PRIMER_PAIR_2_PENALTY=5.325102 PRIMER_LEFT_2_PENALTY=4.110393 PRIMER_RIGHT_2_PENALTY=1.214710 PRIMER_LEFT_2_SEQUENCE=CTTACTTACACACCACACAGACCC PRIMER_RIGHT_2_SEQUENCE=GACGTGTGTGTGTGTATTGGG PRIMER_LEFT_2=105,24 PRIMER_RIGHT_2=233,21 PRIMER_LEFT_2_TM=59.890 PRIMER_RIGHT_2_TM=59.785 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.1000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=10.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=129 PRIMER_PAIR_3_PENALTY=5.705479 PRIMER_LEFT_3_PENALTY=4.490770 PRIMER_RIGHT_3_PENALTY=1.214710 PRIMER_LEFT_3_SEQUENCE=TACTTACACACCACACAGACCC PRIMER_RIGHT_3_SEQUENCE=GACGTGTGTGTGTGTATTGGG PRIMER_LEFT_3=107,22 PRIMER_RIGHT_3=233,21 PRIMER_LEFT_3_TM=57.509 PRIMER_RIGHT_3_TM=59.785 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.1000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=10.00 PRIMER_PAIR_3_COMPL_END=6.00 PRIMER_PAIR_3_PRODUCT_SIZE=127 PRIMER_PAIR_4_PENALTY=5.773868 PRIMER_LEFT_4_PENALTY=0.063544 PRIMER_RIGHT_4_PENALTY=5.710325 PRIMER_LEFT_4_SEQUENCE=CACCACACAGACCCACTACG PRIMER_RIGHT_4_SEQUENCE=CGAAGTAGAGAGTAGACGTGTGTG PRIMER_LEFT_4=115,20 PRIMER_RIGHT_4=247,24 PRIMER_LEFT_4_TM=60.064 PRIMER_RIGHT_4_TM=58.290 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=133 = SEQUENCE_ID=37.RHAP34FH1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCTGCATCTCCATACTCCATTGATGTAGTGCTAGGGACAGAATTCAGGGCATCATGCATGCTACAAACTAAGCTACATCCCTAGCCCCACATGTGAAGTCTTTTGTAACTGATGTCTACTGATTTGAACCTAAGGAGGCTATAAGAGAAGAGAACCACTGAACTCCATGAGATTTAGAACAGGAAATGGAATGGAGACTATTATTTTTTCCACTGTTTCTCTGTTTCTCTGTGTTTCTCTTANNNNNNNNNNNNNNNNNNNNTCTGTCCTGTTTAAGTGTGGA SEQUENCE_INCLUDED_REGION=18,283 SEQUENCE_TARGET=260,20 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=38.RHAP34FH2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCTGGGTTCGGTCCCCAGCTCCGGGGGGAAAAAAAAAAAAGAATTGCAGGTCAGTGTCATACAGGTCTTGGTGGTCTGTTTTCTTTAGTCTGTTTTATTCCATGCTGTCACACAGTAAAGATGCAGTCTACTACTGAGTTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTATCCACATGCCATAGAATGTGTGTTGGAGGTCTGAAGAATAGCTTTCAGAGAGTTGGTTCATCATCTTTCATCATATTTTTTTTCCCCAGAAATTG SEQUENCE_INCLUDED_REGION=20,282 SEQUENCE_TARGET=162,40 P3_COMMENT=CL is TGCAGGTCAGTGTCATACAGG P3_COMMENT=CR is TCCAACACACATTCTATGGCA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.259669 PRIMER_LEFT_0_PENALTY=1.239519 PRIMER_RIGHT_0_PENALTY=1.020151 PRIMER_LEFT_0_SEQUENCE=TGCAGGTCAGTGTCATACAGG PRIMER_RIGHT_0_SEQUENCE=TCCAACACACATTCTATGGCA PRIMER_LEFT_0=64,21 PRIMER_RIGHT_0=233,21 PRIMER_LEFT_0_TM=59.760 PRIMER_RIGHT_0_TM=59.980 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=10.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=170 PRIMER_PAIR_1_PENALTY=2.922717 PRIMER_LEFT_1_PENALTY=1.902566 PRIMER_RIGHT_1_PENALTY=1.020151 PRIMER_LEFT_1_SEQUENCE=CAGGTCTTGGTGGTCTGTTTT PRIMER_RIGHT_1_SEQUENCE=TCCAACACACATTCTATGGCA PRIMER_LEFT_1=81,21 PRIMER_RIGHT_1=233,21 PRIMER_LEFT_1_TM=59.097 PRIMER_RIGHT_1_TM=59.980 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=153 PRIMER_PAIR_2_PENALTY=3.048207 PRIMER_LEFT_2_PENALTY=2.028056 PRIMER_RIGHT_2_PENALTY=1.020151 PRIMER_LEFT_2_SEQUENCE=TCATACAGGTCTTGGTGGTCTG PRIMER_RIGHT_2_SEQUENCE=TCCAACACACATTCTATGGCA PRIMER_LEFT_2=76,22 PRIMER_RIGHT_2=233,21 PRIMER_LEFT_2_TM=60.028 PRIMER_RIGHT_2_TM=59.980 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=158 PRIMER_PAIR_3_PENALTY=3.082112 PRIMER_LEFT_3_PENALTY=2.061961 PRIMER_RIGHT_3_PENALTY=1.020151 PRIMER_LEFT_3_SEQUENCE=ACAGGTCTTGGTGGTCTGTTTT PRIMER_RIGHT_3_SEQUENCE=TCCAACACACATTCTATGGCA PRIMER_LEFT_3=80,22 PRIMER_RIGHT_3=233,21 PRIMER_LEFT_3_TM=59.938 PRIMER_RIGHT_3_TM=59.980 PRIMER_LEFT_3_GC_PERCENT=45.455 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=154 PRIMER_PAIR_4_PENALTY=3.129351 PRIMER_LEFT_4_PENALTY=2.109200 PRIMER_RIGHT_4_PENALTY=1.020151 PRIMER_LEFT_4_SEQUENCE=TGTCATACAGGTCTTGGTGGTC PRIMER_RIGHT_4_SEQUENCE=TCCAACACACATTCTATGGCA PRIMER_LEFT_4=74,22 PRIMER_RIGHT_4=233,21 PRIMER_LEFT_4_TM=59.891 PRIMER_RIGHT_4_TM=59.980 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=10.0000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=160 = SEQUENCE_ID=39.RHAP33FD3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTTTTTGGAACACAAGTGNNNNNNNNNNNNNNNNNNNNAATCCTTTTAAGCCTTTTTAAAGAAATGATTGACATTGTGAATTAGACAATTTTCAGTTATAAATTTTCAGCACACAGCACAGAAAGCCTAAATGTGCAATAAAATGCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,148 SEQUENCE_TARGET=39,20 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=39.RHAP33FH3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNTATTTCTCTGTCTTTTCCTTCTACGTGCTTTCTTTCTTTTTCCCATCTCCCTAAACTCAACCAGGATTTACAGGAGAGAGGGACTTTACCATCTNNNNNNNNNNNNNNNNNNNNCCCACTGCACTCAAGCGCTCGCACGGTGCCCCTCCTCACAGCCTGTGTGCTTTGCTCTCTTCATATATGATTGACAGGGAGAAAAAAAATGAGAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,211 SEQUENCE_TARGET=108,20 P3_COMMENT=CL is CCCATCTCCCTAAACTCAACC P3_COMMENT=CR is GAGAGCAAAGCACACAGGCT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.930203 PRIMER_LEFT_0_PENALTY=1.187326 PRIMER_RIGHT_0_PENALTY=0.742877 PRIMER_LEFT_0_SEQUENCE=CCCATCTCCCTAAACTCAACC PRIMER_RIGHT_0_SEQUENCE=GAGAGCAAAGCACACAGGCT PRIMER_LEFT_0=55,21 PRIMER_RIGHT_0=186,20 PRIMER_LEFT_0_TM=59.813 PRIMER_RIGHT_0_TM=60.743 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=132 PRIMER_PAIR_1_PENALTY=1.930203 PRIMER_LEFT_1_PENALTY=1.187326 PRIMER_RIGHT_1_PENALTY=0.742877 PRIMER_LEFT_1_SEQUENCE=CCCATCTCCCTAAACTCAACC PRIMER_RIGHT_1_SEQUENCE=AGAGAGCAAAGCACACAGGC PRIMER_LEFT_1=55,21 PRIMER_RIGHT_1=187,20 PRIMER_LEFT_1_TM=59.813 PRIMER_RIGHT_1_TM=60.743 PRIMER_LEFT_1_GC_PERCENT=52.381 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=133 PRIMER_PAIR_2_PENALTY=1.966036 PRIMER_LEFT_2_PENALTY=1.223158 PRIMER_RIGHT_2_PENALTY=0.742877 PRIMER_LEFT_2_SEQUENCE=CGTGCTTTCTTTCTTTTTCCC PRIMER_RIGHT_2_SEQUENCE=GAGAGCAAAGCACACAGGCT PRIMER_LEFT_2=37,21 PRIMER_RIGHT_2=186,20 PRIMER_LEFT_2_TM=60.223 PRIMER_RIGHT_2_TM=60.743 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=8.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=150 PRIMER_PAIR_3_PENALTY=1.966036 PRIMER_LEFT_3_PENALTY=1.223158 PRIMER_RIGHT_3_PENALTY=0.742877 PRIMER_LEFT_3_SEQUENCE=CGTGCTTTCTTTCTTTTTCCC PRIMER_RIGHT_3_SEQUENCE=AGAGAGCAAAGCACACAGGC PRIMER_LEFT_3=37,21 PRIMER_RIGHT_3=187,20 PRIMER_LEFT_3_TM=60.223 PRIMER_RIGHT_3_TM=60.743 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=9.7000 PRIMER_PAIR_3_COMPL_ANY=9.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=151 PRIMER_PAIR_4_PENALTY=2.224300 PRIMER_LEFT_4_PENALTY=1.481423 PRIMER_RIGHT_4_PENALTY=0.742877 PRIMER_LEFT_4_SEQUENCE=TCTTTCTTTTTCCCATCTCCC PRIMER_RIGHT_4_SEQUENCE=GAGAGCAAAGCACACAGGCT PRIMER_LEFT_4=44,21 PRIMER_RIGHT_4=186,20 PRIMER_LEFT_4_TM=59.519 PRIMER_RIGHT_4_TM=60.743 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=143 = SEQUENCE_ID=39.RHAP34FH3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCTGTATGGAATAAAGTTTAAACATTTTTAAGTGTAACATAATAGTAAGCATACGACTGAAATGAATGTGATAGAGAAGGAAGTGGTTTGCCCAGCAAGGAATGTCATGACATCATAAAGTTATTCTGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGATTATATACATGCATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTCTGCTTCTTTTATTTCACATACAAAGGCCGTACTCTTGGAACTGTGAGTCAAAATAAGGACTTTGNCCNTG SEQUENCE_INCLUDED_REGION=18,286 SEQUENCE_TARGET=148,32 SEQUENCE_TARGET=202,28 P3_COMMENT=CL is CCCAGCAAGGAATGTCATG P3_COMMENT=CR is TTCCAAGAGTACGGCCTTTG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.296841 PRIMER_LEFT_0_PENALTY=1.055809 PRIMER_RIGHT_0_PENALTY=0.241032 PRIMER_LEFT_0_SEQUENCE=CCCAGCAAGGAATGTCATG PRIMER_RIGHT_0_SEQUENCE=TTCCAAGAGTACGGCCTTTG PRIMER_LEFT_0=108,19 PRIMER_RIGHT_0=273,20 PRIMER_LEFT_0_TM=60.056 PRIMER_RIGHT_0_TM=60.241 PRIMER_LEFT_0_GC_PERCENT=52.632 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=166 PRIMER_PAIR_1_PENALTY=1.321524 PRIMER_LEFT_1_PENALTY=1.055809 PRIMER_RIGHT_1_PENALTY=0.265715 PRIMER_LEFT_1_SEQUENCE=CCCAGCAAGGAATGTCATG PRIMER_RIGHT_1_SEQUENCE=TCCAAGAGTACGGCCTTTGT PRIMER_LEFT_1=108,19 PRIMER_RIGHT_1=272,20 PRIMER_LEFT_1_TM=60.056 PRIMER_RIGHT_1_TM=59.734 PRIMER_LEFT_1_GC_PERCENT=52.632 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=165 PRIMER_PAIR_2_PENALTY=1.703177 PRIMER_LEFT_2_PENALTY=1.055809 PRIMER_RIGHT_2_PENALTY=0.647368 PRIMER_LEFT_2_SEQUENCE=CCCAGCAAGGAATGTCATG PRIMER_RIGHT_2_SEQUENCE=CAGTTCCAAGAGTACGGCCT PRIMER_LEFT_2=108,19 PRIMER_RIGHT_2=276,20 PRIMER_LEFT_2_TM=60.056 PRIMER_RIGHT_2_TM=59.353 PRIMER_LEFT_2_GC_PERCENT=52.632 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=10.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=169 PRIMER_PAIR_3_PENALTY=1.844043 PRIMER_LEFT_3_PENALTY=1.055809 PRIMER_RIGHT_3_PENALTY=0.788233 PRIMER_LEFT_3_SEQUENCE=CCCAGCAAGGAATGTCATG PRIMER_RIGHT_3_SEQUENCE=ACAGTTCCAAGAGTACGGCC PRIMER_LEFT_3=108,19 PRIMER_RIGHT_3=277,20 PRIMER_LEFT_3_TM=60.056 PRIMER_RIGHT_3_TM=59.212 PRIMER_LEFT_3_GC_PERCENT=52.632 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=12.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=170 PRIMER_PAIR_4_PENALTY=2.237217 PRIMER_LEFT_4_PENALTY=1.055809 PRIMER_RIGHT_4_PENALTY=1.181407 PRIMER_LEFT_4_SEQUENCE=CCCAGCAAGGAATGTCATG PRIMER_RIGHT_4_SEQUENCE=ACAGTTCCAAGAGTACGGCCT PRIMER_LEFT_4=108,19 PRIMER_RIGHT_4=277,21 PRIMER_LEFT_4_TM=60.056 PRIMER_RIGHT_4_TM=60.181 PRIMER_LEFT_4_GC_PERCENT=52.632 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=10.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=170 = SEQUENCE_ID=40.RHAP33FD4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTCCGAAAAAAAGAAAAGAAAAAAAATTTAGTGATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCACAGATATCAGATTTTAATATGTGCTCTTCTTACTCACACTCTTCCACCTACCTTTCCTCCCTGGCGAACTTCTGCCCGGCTACTCTCTCCTCATTCCCACCCCAGATAATAGGTCTTTCCTTGCCTCTGTGCCTCTATGGCAATATGAATCTAGTTCTATTCTAACATGCTTTTGTTGATAATCATGCTTTGGGGGTATCTTCCATCCTGTCCACAAGCCTTTTCCCATAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,305 SEQUENCE_TARGET=61,32 P3_COMMENT=CL is TCCGAAAAAAAGAAAAGAAAAAAA P3_COMMENT=CR is AGGGAGGAAAGGTAGGTGGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.394016 PRIMER_LEFT_0_PENALTY=4.322131 PRIMER_RIGHT_0_PENALTY=0.071885 PRIMER_LEFT_0_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA PRIMER_RIGHT_0_SEQUENCE=AGGGAGGAAAGGTAGGTGGA PRIMER_LEFT_0=24,24 PRIMER_RIGHT_0=158,20 PRIMER_LEFT_0_TM=59.678 PRIMER_RIGHT_0_TM=59.928 PRIMER_LEFT_0_GC_PERCENT=20.833 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.6000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=135 PRIMER_PAIR_1_PENALTY=4.508176 PRIMER_LEFT_1_PENALTY=4.436291 PRIMER_RIGHT_1_PENALTY=0.071885 PRIMER_LEFT_1_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAA PRIMER_RIGHT_1_SEQUENCE=AGGGAGGAAAGGTAGGTGGA PRIMER_LEFT_1=24,23 PRIMER_RIGHT_1=158,20 PRIMER_LEFT_1_TM=58.564 PRIMER_RIGHT_1_TM=59.928 PRIMER_LEFT_1_GC_PERCENT=21.739 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=135 PRIMER_PAIR_2_PENALTY=4.620407 PRIMER_LEFT_2_PENALTY=4.322131 PRIMER_RIGHT_2_PENALTY=0.298276 PRIMER_LEFT_2_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA PRIMER_RIGHT_2_SEQUENCE=GGGAGGAAAGGTAGGTGGAA PRIMER_LEFT_2=24,24 PRIMER_RIGHT_2=157,20 PRIMER_LEFT_2_TM=59.678 PRIMER_RIGHT_2_TM=60.298 PRIMER_LEFT_2_GC_PERCENT=20.833 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.6000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=134 PRIMER_PAIR_3_PENALTY=4.693497 PRIMER_LEFT_3_PENALTY=4.621612 PRIMER_RIGHT_3_PENALTY=0.071885 PRIMER_LEFT_3_SEQUENCE=CTCCGAAAAAAAGAAAAGAAAAAA PRIMER_RIGHT_3_SEQUENCE=AGGGAGGAAAGGTAGGTGGA PRIMER_LEFT_3=23,24 PRIMER_RIGHT_3=158,20 PRIMER_LEFT_3_TM=59.378 PRIMER_RIGHT_3_TM=59.928 PRIMER_LEFT_3_GC_PERCENT=25.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=136 PRIMER_PAIR_4_PENALTY=4.725092 PRIMER_LEFT_4_PENALTY=4.322131 PRIMER_RIGHT_4_PENALTY=0.402961 PRIMER_LEFT_4_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA PRIMER_RIGHT_4_SEQUENCE=AGAGTAGCCGGGCAGAAGTT PRIMER_LEFT_4=24,24 PRIMER_RIGHT_4=182,20 PRIMER_LEFT_4_TM=59.678 PRIMER_RIGHT_4_TM=60.403 PRIMER_LEFT_4_GC_PERCENT=20.833 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.6000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=159 = SEQUENCE_ID=40.RHAP33FH4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCTCCCACCTTCCTCATGTTGCCTTGCTCCAGTGGACAGCCAATTATGACAGATGATGGTGGAGGGACAAGATGTGCCCATATCCCCTCTCCCTTCATATCTTCTACTTCAGCAAGGGAGAGGCTAGCAGAGGGGGTTGAGTGTGTGTTCAGTAAGAAGGGACAGAGTAGTGCTAATGTGGGTGGCTTTCCCCACTGTTCTGGGGAAGACTGAANNNNNNNNNNNNNNNNNNNNNNTACAGAACTCAAAGTCTTTAATTCTGTGATGCATGGTAGAATTCATAGTAATGATAGAAAAATGGTTATTTTATTTTCTGTTCTAGNANGTNGGAAGGCATCGTGAACNAGTTTGNAGGAAAAAAGANCATT SEQUENCE_INCLUDED_REGION=15,367 SEQUENCE_TARGET=228,22 P3_COMMENT=CL is AAGGGAGAGGCTAGCAGAGG P3_COMMENT=CR is TGAATTCTACCATGCATCACAG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.458937 PRIMER_LEFT_0_PENALTY=0.110131 PRIMER_RIGHT_0_PENALTY=3.348806 PRIMER_LEFT_0_SEQUENCE=AAGGGAGAGGCTAGCAGAGG PRIMER_RIGHT_0_SEQUENCE=TGAATTCTACCATGCATCACAG PRIMER_LEFT_0=127,20 PRIMER_RIGHT_0=294,22 PRIMER_LEFT_0_TM=60.110 PRIMER_RIGHT_0_TM=58.651 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=168 PRIMER_PAIR_1_PENALTY=3.488065 PRIMER_LEFT_1_PENALTY=0.139259 PRIMER_RIGHT_1_PENALTY=3.348806 PRIMER_LEFT_1_SEQUENCE=CTAGCAGAGGGGGTTGAGTG PRIMER_RIGHT_1_SEQUENCE=TGAATTCTACCATGCATCACAG PRIMER_LEFT_1=137,20 PRIMER_RIGHT_1=294,22 PRIMER_LEFT_1_TM=59.861 PRIMER_RIGHT_1_TM=58.651 PRIMER_LEFT_1_GC_PERCENT=60.000 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=158 PRIMER_PAIR_2_PENALTY=3.489891 PRIMER_LEFT_2_PENALTY=0.141085 PRIMER_RIGHT_2_PENALTY=3.348806 PRIMER_LEFT_2_SEQUENCE=AGGGGGTTGAGTGTGTGTTC PRIMER_RIGHT_2_SEQUENCE=TGAATTCTACCATGCATCACAG PRIMER_LEFT_2=144,20 PRIMER_RIGHT_2=294,22 PRIMER_LEFT_2_TM=59.859 PRIMER_RIGHT_2_TM=58.651 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=151 PRIMER_PAIR_3_PENALTY=3.489891 PRIMER_LEFT_3_PENALTY=0.141085 PRIMER_RIGHT_3_PENALTY=3.348806 PRIMER_LEFT_3_SEQUENCE=GAGGGGGTTGAGTGTGTGTT PRIMER_RIGHT_3_SEQUENCE=TGAATTCTACCATGCATCACAG PRIMER_LEFT_3=143,20 PRIMER_RIGHT_3=294,22 PRIMER_LEFT_3_TM=59.859 PRIMER_RIGHT_3_TM=58.651 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=1.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=152 PRIMER_PAIR_4_PENALTY=3.554405 PRIMER_LEFT_4_PENALTY=0.110131 PRIMER_RIGHT_4_PENALTY=3.444274 PRIMER_LEFT_4_SEQUENCE=AAGGGAGAGGCTAGCAGAGG PRIMER_RIGHT_4_SEQUENCE=TGAATTCTACCATGCATCACA PRIMER_LEFT_4=127,20 PRIMER_RIGHT_4=294,21 PRIMER_LEFT_4_TM=60.110 PRIMER_RIGHT_4_TM=57.556 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=168 = SEQUENCE_ID=40.RHAP34FH4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCAACTGAGGCTTCTACTGTGACTGCATTAAAGCTCAGCTTGTTCCCTCTGCTTCCATGGGTTTTTATTCCCCCAAAGCAACATTTGCTATACCTACCTCTGGTCTTTAACTGGTATCAGCAGTCTCAGAACAGCAACCCGAGGTTAGGGTGTTACGCCTAGCTAGCCCCGCTGGCAGTAAAGAACCCACGACAGTGTGGGTGGGGCCAGCTATTTCCAAATGCCATGTGGTACAGAAGGTAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,283 SEQUENCE_TARGET=264,38 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=41.RHAP33FD5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCTACAGTGTTTGGAGTTTGTCCTGACAGGTTTCTATTTTGCTCTGATGTAATATTTATTCATTATATCCCCTTTGCTGCCTTTATAACAGTTATGTGTATTCTGTGCCATCATCTGGTAGAAGTATGTCATTTGCTTTTTTAATTTTGATTTTACAGGGTATTATGATTAAAAGCCTGGAGTATCAGAAGACTCTGGACTTTTTAAACTGTACTGGATCAAATGCCTTTTGTNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=22,248 SEQUENCE_TARGET=254,15 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=41.RHAP33FH5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNTGACCAATCAGCATGTTTTATGTTAAGTTGAAAAGACACTGTGTTGTATGGATTACAGATTTGCTAGGTGGTTAAATATTCTTGGATTTTGGTAACACAGAGGCACATGTGCACGTGCACACATATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGATGGGGGGTGTTAATGGGGGAGGGAGAAAGNAGGAAGCAAGAAGCNATCCCTAACTGTAGTGAGGCAGGTAATGNAGAAAAGTGTAACAGATACCGACCTTTAGACAAGACTGCAGATATTAGANAAGCGATGGCTCTGTAATATAAATTTGTTAAGACATAGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,343 SEQUENCE_TARGET=142,42 P3_COMMENT=CL is CTTGGATTTTGGTAACACAGAGG P3_COMMENT=CR is CTTTCTCCCTCCCCCATTAA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.336015 PRIMER_LEFT_0_PENALTY=3.085878 PRIMER_RIGHT_0_PENALTY=0.250137 PRIMER_LEFT_0_SEQUENCE=CTTGGATTTTGGTAACACAGAGG PRIMER_RIGHT_0_SEQUENCE=CTTTCTCCCTCCCCCATTAA PRIMER_LEFT_0=94,23 PRIMER_RIGHT_0=218,20 PRIMER_LEFT_0_TM=59.914 PRIMER_RIGHT_0_TM=60.250 PRIMER_LEFT_0_GC_PERCENT=43.478 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=125 PRIMER_PAIR_1_PENALTY=3.493328 PRIMER_LEFT_1_PENALTY=3.369403 PRIMER_RIGHT_1_PENALTY=0.123924 PRIMER_LEFT_1_SEQUENCE=TGGATTACAGATTTGCTAGGTGG PRIMER_RIGHT_1_SEQUENCE=TTTCTCCCTCCCCCATTAAC PRIMER_LEFT_1=62,23 PRIMER_RIGHT_1=217,20 PRIMER_LEFT_1_TM=60.369 PRIMER_RIGHT_1_TM=60.124 PRIMER_LEFT_1_GC_PERCENT=43.478 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=6.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=156 PRIMER_PAIR_2_PENALTY=3.502257 PRIMER_LEFT_2_PENALTY=1.375502 PRIMER_RIGHT_2_PENALTY=2.126755 PRIMER_LEFT_2_SEQUENCE=TTGGTAACACAGAGGCACATG PRIMER_RIGHT_2_SEQUENCE=CCTGCCTCACTACAGTTAGGG PRIMER_LEFT_2=102,21 PRIMER_RIGHT_2=257,21 PRIMER_LEFT_2_TM=59.624 PRIMER_RIGHT_2_TM=58.873 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=57.143 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=8.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=156 PRIMER_PAIR_3_PENALTY=3.619540 PRIMER_LEFT_3_PENALTY=3.369403 PRIMER_RIGHT_3_PENALTY=0.250137 PRIMER_LEFT_3_SEQUENCE=TGGATTACAGATTTGCTAGGTGG PRIMER_RIGHT_3_SEQUENCE=CTTTCTCCCTCCCCCATTAA PRIMER_LEFT_3=62,23 PRIMER_RIGHT_3=218,20 PRIMER_LEFT_3_TM=60.369 PRIMER_RIGHT_3_TM=60.250 PRIMER_LEFT_3_GC_PERCENT=43.478 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=6.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=157 PRIMER_PAIR_4_PENALTY=3.676869 PRIMER_LEFT_4_PENALTY=1.375502 PRIMER_RIGHT_4_PENALTY=2.301367 PRIMER_LEFT_4_SEQUENCE=TTGGTAACACAGAGGCACATG PRIMER_RIGHT_4_SEQUENCE=ACCTGCCTCACTACAGTTAGGG PRIMER_LEFT_4=102,21 PRIMER_RIGHT_4=258,22 PRIMER_LEFT_4_TM=59.624 PRIMER_RIGHT_4_TM=59.699 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=54.545 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=8.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=157 = SEQUENCE_ID=41.RHAP34FD5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNATATATTCTATGTATCTATGCNNNNNNNNNNNNNNNNNNNNAATTTATTTATTATTTTACCTTTGGGAAATACTCTCTTTGCAAAGCTTATAATGACTCCATGATAATTAAAAGCAGCAGGGGTTCAAGAGGCAAGAATGCATCTAGGTCTTAGCAAAATGGCAAATGCTGAGACCTTCAAGACCGTCATTAAGGCTGTGGGAATGAACTCTGAAAACATGTAGTTCAAATATAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,236 SEQUENCE_TARGET=40,20 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=41.RHAP34FH5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCAGGCATCTCTGAGGGAAAGCAGGAGAAGGAGTCTAATTCCTCTGCAAACTAATTAATGAGCCTTCCCAGAGTGCTGGCAGCTAACAGGCAAAACTGAGAACCACTAACAGCAAGTACACCAAAGCCAGTAAGTAGCAATGCTTTCCAGGTGTGTGCATGTATACCNNNNNNNNNNNNNNNNNNNNNNNNNNCTAGAGCACCCATGCATGTCATAGTGTGCATGGAGGTCAGAGGACAGCCCTGCCTTCTGCTCTTTGCCTTTCACTCTGTTTCAGACAGAC SEQUENCE_INCLUDED_REGION=18,282 SEQUENCE_TARGET=184,26 P3_COMMENT=CL is CAGCAAGTACACCAAAGCCA P3_COMMENT=CR is GTCCTCTGACCTCCATGCAC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.784472 PRIMER_LEFT_0_PENALTY=0.095506 PRIMER_RIGHT_0_PENALTY=0.688966 PRIMER_LEFT_0_SEQUENCE=CAGCAAGTACACCAAAGCCA PRIMER_RIGHT_0_SEQUENCE=GTCCTCTGACCTCCATGCAC PRIMER_LEFT_0=126,20 PRIMER_RIGHT_0=254,20 PRIMER_LEFT_0_TM=59.904 PRIMER_RIGHT_0_TM=60.689 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=129 PRIMER_PAIR_1_PENALTY=0.794904 PRIMER_LEFT_1_PENALTY=0.347100 PRIMER_RIGHT_1_PENALTY=0.447804 PRIMER_LEFT_1_SEQUENCE=CAGAGTGCTGGCAGCTAACA PRIMER_RIGHT_1_SEQUENCE=CTATGACATGCATGGGTGCT PRIMER_LEFT_1=85,20 PRIMER_RIGHT_1=233,20 PRIMER_LEFT_1_TM=60.347 PRIMER_RIGHT_1_TM=59.552 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.0000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=149 PRIMER_PAIR_2_PENALTY=0.860552 PRIMER_LEFT_2_PENALTY=0.347100 PRIMER_RIGHT_2_PENALTY=0.513452 PRIMER_LEFT_2_SEQUENCE=CAGAGTGCTGGCAGCTAACA PRIMER_RIGHT_2_SEQUENCE=TATGACATGCATGGGTGCTC PRIMER_LEFT_2=85,20 PRIMER_RIGHT_2=232,20 PRIMER_LEFT_2_TM=60.347 PRIMER_RIGHT_2_TM=60.513 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.0000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=148 PRIMER_PAIR_3_PENALTY=0.900981 PRIMER_LEFT_3_PENALTY=0.095506 PRIMER_RIGHT_3_PENALTY=0.805475 PRIMER_LEFT_3_SEQUENCE=CAGCAAGTACACCAAAGCCA PRIMER_RIGHT_3_SEQUENCE=GAAAGGCAAAGAGCAGAAGG PRIMER_LEFT_3=126,20 PRIMER_RIGHT_3=281,20 PRIMER_LEFT_3_TM=59.904 PRIMER_RIGHT_3_TM=59.195 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_4_PENALTY=0.924142 PRIMER_LEFT_4_PENALTY=0.095506 PRIMER_RIGHT_4_PENALTY=0.828636 PRIMER_LEFT_4_SEQUENCE=CAGCAAGTACACCAAAGCCA PRIMER_RIGHT_4_SEQUENCE=CTGTCCTCTGACCTCCATGC PRIMER_LEFT_4=126,20 PRIMER_RIGHT_4=256,20 PRIMER_LEFT_4_TM=59.904 PRIMER_RIGHT_4_TM=60.829 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=8.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=131 = SEQUENCE_ID=43.RHAP34FD7.seq SEQUENCE_TEMPLATE=ANACCCCACCNCCAGCNCCNCACACCNCAACCAGAACNGGTCNNNCNNTNTNNGTGCNNNNNNNNNNNNNNNNATCNCNCNGTGTGTGCCCNGCTCNGTATCNGTCNGNGTGTCNNGCCTCGCNNNNNNNNNNNNNNNNNNNNNNNNNCTGCGTGTCTCGTCGTGTGTGATTGATGTTGTGNGTCNTGTCGTGATGCTGNTCGTCTCTGATGTGNTNNGNNGNNTGNTGTTGNTAGNTNNTAGTGNNGNTANNGNTNCNNGCTCNTGNTNNNGNTCCNNGNTNNTCANNGANGNNGATNCTCGCTCNTNGNTGNTGATGNNGCACCNGNTGTNGCNGATA SEQUENCE_INCLUDED_REGION=0,340 SEQUENCE_TARGET=59,14 SEQUENCE_TARGET=124,24 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=44.RHAP33FD8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNTGCCATATTTCCCNAGCCATTAACTCATCTGACATAGGCCTTCTTCACGTTGCATCTAGATAGGTCTTCCTGTCTTCCTCTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGAGAGAGAGAAGGAGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,179 SEQUENCE_TARGET=95,56 SEQUENCE_TARGET=174,16 P3_COMMENT=CL is GCCTTCTTCACGTTGCATCT P3_COMMENT=CR is CCCCCTCCTTCTCTCTCTCT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.426207 PRIMER_LEFT_0_PENALTY=0.405717 PRIMER_RIGHT_0_PENALTY=1.020490 PRIMER_LEFT_0_SEQUENCE=GCCTTCTTCACGTTGCATCT PRIMER_RIGHT_0_SEQUENCE=CCCCCTCCTTCTCTCTCTCT PRIMER_LEFT_0=48,20 PRIMER_RIGHT_0=172,20 PRIMER_LEFT_0_TM=60.406 PRIMER_RIGHT_0_TM=58.980 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=1.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=125 PRIMER_PAIR_1_PENALTY=1.660714 PRIMER_LEFT_1_PENALTY=0.405717 PRIMER_RIGHT_1_PENALTY=1.254998 PRIMER_LEFT_1_SEQUENCE=GCCTTCTTCACGTTGCATCT PRIMER_RIGHT_1_SEQUENCE=CCCCCCTCCTTCTCTCTCT PRIMER_LEFT_1=48,20 PRIMER_RIGHT_1=173,19 PRIMER_LEFT_1_TM=60.406 PRIMER_RIGHT_1_TM=59.745 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=63.158 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=0.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=1.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=126 PRIMER_PAIR_2_PENALTY=1.680443 PRIMER_LEFT_2_PENALTY=0.659953 PRIMER_RIGHT_2_PENALTY=1.020490 PRIMER_LEFT_2_SEQUENCE=ATAGGCCTTCTTCACGTTGC PRIMER_RIGHT_2_SEQUENCE=CCCCCTCCTTCTCTCTCTCT PRIMER_LEFT_2=44,20 PRIMER_RIGHT_2=172,20 PRIMER_LEFT_2_TM=59.340 PRIMER_RIGHT_2_TM=58.980 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=0.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=129 PRIMER_PAIR_3_PENALTY=1.728423 PRIMER_LEFT_3_PENALTY=0.405717 PRIMER_RIGHT_3_PENALTY=1.322707 PRIMER_LEFT_3_SEQUENCE=GCCTTCTTCACGTTGCATCT PRIMER_RIGHT_3_SEQUENCE=CCCCCTCCTTCTCTCTCTCTT PRIMER_LEFT_3=48,20 PRIMER_RIGHT_3=172,21 PRIMER_LEFT_3_TM=60.406 PRIMER_RIGHT_3_TM=60.323 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=57.143 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=0.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=1.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=125 PRIMER_PAIR_4_PENALTY=1.914951 PRIMER_LEFT_4_PENALTY=0.659953 PRIMER_RIGHT_4_PENALTY=1.254998 PRIMER_LEFT_4_SEQUENCE=ATAGGCCTTCTTCACGTTGC PRIMER_RIGHT_4_SEQUENCE=CCCCCCTCCTTCTCTCTCT PRIMER_LEFT_4=44,20 PRIMER_RIGHT_4=173,19 PRIMER_LEFT_4_TM=59.340 PRIMER_RIGHT_4_TM=59.745 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=63.158 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=0.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=130 = SEQUENCE_ID=44.RHAP34FD8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNACTCCAAATAGTGCCTACATCCCATATTATTCTATCTTTGCTATCAATTGATTTTTTTCACAAAGTTTCTCTTCCTCCCAAATTAGAATGATTTCTCTCCCTTGTTTGGTTGTATGTTCATTCTTAAGCTGTAGTTAACACTGTGCCTTAGAACATCTTATTACAAGATCCCATTTAGCATTTACATAACCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAGCCATGGAGCACTATTATTTGTTGGTAATTGNTTAAATGCAAAAAACAATGTCCCTTG SEQUENCE_INCLUDED_REGION=11,284 SEQUENCE_TARGET=204,30 P3_COMMENT=CL is TTTCTCTCCCTTGTTTGGTTG P3_COMMENT=CR is CAAATAATAGTGCTCCATGGCTT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.246089 PRIMER_LEFT_0_PENALTY=1.799253 PRIMER_RIGHT_0_PENALTY=3.446835 PRIMER_LEFT_0_SEQUENCE=TTTCTCTCCCTTGTTTGGTTG PRIMER_RIGHT_0_SEQUENCE=CAAATAATAGTGCTCCATGGCTT PRIMER_LEFT_0=102,21 PRIMER_RIGHT_0=257,23 PRIMER_LEFT_0_TM=59.201 PRIMER_RIGHT_0_TM=59.553 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=39.130 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=156 PRIMER_PAIR_1_PENALTY=5.290334 PRIMER_LEFT_1_PENALTY=1.843499 PRIMER_RIGHT_1_PENALTY=3.446835 PRIMER_LEFT_1_SEQUENCE=TGATTTCTCTCCCTTGTTTGG PRIMER_RIGHT_1_SEQUENCE=CAAATAATAGTGCTCCATGGCTT PRIMER_LEFT_1=99,21 PRIMER_RIGHT_1=257,23 PRIMER_LEFT_1_TM=59.157 PRIMER_RIGHT_1_TM=59.553 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=39.130 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=159 PRIMER_PAIR_2_PENALTY=5.366867 PRIMER_LEFT_2_PENALTY=1.799253 PRIMER_RIGHT_2_PENALTY=3.567613 PRIMER_LEFT_2_SEQUENCE=TTTCTCTCCCTTGTTTGGTTG PRIMER_RIGHT_2_SEQUENCE=AACAAATAATAGTGCTCCATGGC PRIMER_LEFT_2=102,21 PRIMER_RIGHT_2=259,23 PRIMER_LEFT_2_TM=59.201 PRIMER_RIGHT_2_TM=59.432 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=158 PRIMER_PAIR_3_PENALTY=5.411112 PRIMER_LEFT_3_PENALTY=1.843499 PRIMER_RIGHT_3_PENALTY=3.567613 PRIMER_LEFT_3_SEQUENCE=TGATTTCTCTCCCTTGTTTGG PRIMER_RIGHT_3_SEQUENCE=AACAAATAATAGTGCTCCATGGC PRIMER_LEFT_3=99,21 PRIMER_RIGHT_3=259,23 PRIMER_LEFT_3_TM=59.157 PRIMER_RIGHT_3_TM=59.432 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=8.8000 PRIMER_RIGHT_3_END_STABILITY=9.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=161 PRIMER_PAIR_4_PENALTY=5.458989 PRIMER_LEFT_4_PENALTY=2.012153 PRIMER_RIGHT_4_PENALTY=3.446835 PRIMER_LEFT_4_SEQUENCE=TTTCTCTCCCTTGTTTGGTTGT PRIMER_RIGHT_4_SEQUENCE=CAAATAATAGTGCTCCATGGCTT PRIMER_LEFT_4=102,22 PRIMER_RIGHT_4=257,23 PRIMER_LEFT_4_TM=60.012 PRIMER_RIGHT_4_TM=59.553 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=156 = SEQUENCE_ID=45.RHAP33FD9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCTCGTTATGAAAAATTACATCTAATTGGAAGGTAGTTGAGTGAGTGGAGTGAAGTTGAGTTCAGTTCATTTCAGTGAGTCAGTGCAGTGGAATTGTGTTCATGCAATTCAGTGTAGGTCAGCTGGAGGCCCTTGTCCAGGCCACATGTAATACAGAAACAAAAATAGGGAATAGTACCAAGGAACTCTCACTAGAATAAAAGCAAGGAGTGTCCGCAGCACTGTGTTTATTCCTAATGNNNNNNNNNNNNNNNNGCCTCCCAAGGAGAGTCTGCCATGACCCCAGGTAAGAACTCACGCTGCATATGCCAATCCTTTGCTAGAGCATGTGAGCACCACGCCCACTCGACACAAACTTT SEQUENCE_INCLUDED_REGION=11,358 SEQUENCE_TARGET=249,16 P3_COMMENT=CL is TAAAAGCAAGGAGTGTCCGC P3_COMMENT=CR is AAAGTTTGTGTCGAGTGGGC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.542573 PRIMER_LEFT_0_PENALTY=0.386286 PRIMER_RIGHT_0_PENALTY=0.156287 PRIMER_LEFT_0_SEQUENCE=TAAAAGCAAGGAGTGTCCGC PRIMER_RIGHT_0_SEQUENCE=AAAGTTTGTGTCGAGTGGGC PRIMER_LEFT_0=207,20 PRIMER_RIGHT_0=368,20 PRIMER_LEFT_0_TM=60.386 PRIMER_RIGHT_0_TM=60.156 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=11.4000 PRIMER_RIGHT_0_END_STABILITY=11.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=162 PRIMER_PAIR_1_PENALTY=0.554311 PRIMER_LEFT_1_PENALTY=0.386286 PRIMER_RIGHT_1_PENALTY=0.168025 PRIMER_LEFT_1_SEQUENCE=TAAAAGCAAGGAGTGTCCGC PRIMER_RIGHT_1_SEQUENCE=GGTGCTCACATGCTCTAGCA PRIMER_LEFT_1=207,20 PRIMER_RIGHT_1=346,20 PRIMER_LEFT_1_TM=60.386 PRIMER_RIGHT_1_TM=60.168 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=11.4000 PRIMER_RIGHT_1_END_STABILITY=7.5000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=140 PRIMER_PAIR_2_PENALTY=0.554311 PRIMER_LEFT_2_PENALTY=0.386286 PRIMER_RIGHT_2_PENALTY=0.168025 PRIMER_LEFT_2_SEQUENCE=TAAAAGCAAGGAGTGTCCGC PRIMER_RIGHT_2_SEQUENCE=TGGTGCTCACATGCTCTAGC PRIMER_LEFT_2=207,20 PRIMER_RIGHT_2=347,20 PRIMER_LEFT_2_TM=60.386 PRIMER_RIGHT_2_TM=60.168 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=11.4000 PRIMER_RIGHT_2_END_STABILITY=7.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=141 PRIMER_PAIR_3_PENALTY=0.809785 PRIMER_LEFT_3_PENALTY=0.653497 PRIMER_RIGHT_3_PENALTY=0.156287 PRIMER_LEFT_3_SEQUENCE=CGCAGCACTGTGTTTATTCC PRIMER_RIGHT_3_SEQUENCE=AAAGTTTGTGTCGAGTGGGC PRIMER_LEFT_3=224,20 PRIMER_RIGHT_3=368,20 PRIMER_LEFT_3_TM=59.347 PRIMER_RIGHT_3_TM=60.156 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=11.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=145 PRIMER_PAIR_4_PENALTY=0.809785 PRIMER_LEFT_4_PENALTY=0.653497 PRIMER_RIGHT_4_PENALTY=0.156287 PRIMER_LEFT_4_SEQUENCE=CCGCAGCACTGTGTTTATTC PRIMER_RIGHT_4_SEQUENCE=AAAGTTTGTGTCGAGTGGGC PRIMER_LEFT_4=223,20 PRIMER_RIGHT_4=368,20 PRIMER_LEFT_4_TM=59.347 PRIMER_RIGHT_4_TM=60.156 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=5.9000 PRIMER_RIGHT_4_END_STABILITY=11.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=146 = SEQUENCE_ID=46.RHAP33FH10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNACCTGAGAAATTACCTTGGGTTTGGTAGAACCAGGCAGGAATGAATGATGAAGAAATAAGTGAATAGGTCTTCAGCAGAGGGATTTCTGACCTTCTAGCTATTCATCACTTCTCATAAGTATCTAAGCTTTATAAGTGAGTTTTATCCAGTATGTCATGAGCTCGTGTTTATTTATATGTATTTGGATAAGCGTAAATACAAATGAACACTAATAAAAATACTTGTTAAAAATAGAAATCAAACAAATACACAAAGCAAAGAGATATNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNCGTTTTTATATGANNNCAACCNACCNNGGNAGAAANAANGTNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=26,355 SEQUENCE_TARGET=293,27 SEQUENCE_TARGET=321,19 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=46.RHAP34FD10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCTGCTGGATCTGTTTAGTGTTGTTCACGTGCATATGTTTCTAGGCCTAACCACTTACTAGGTATTGGATAATGGATTAGGGCTCAAACCTGGGATGACTGATACTCCTTTTCTCAATAGTTGGATAATGGATTAGGAGCTCAAACCTGGAGAAGACTAATACTCCTTCTCTCAGTAGTTGGATAATAGATTAGGGGCTCAAACCTGGAGAAGACTAATACTCC SEQUENCE_INCLUDED_REGION=0,281 SEQUENCE_TARGET=0,56 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=47.RHAP33FD11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCTGTTTTNGACCTCTTCCTCCTGGAAGGTCAAACTCTAAAGAGGGAACTATAGGGAAGAGTTAGTGGGGCCTTTAAATTGAGCAGCAACCACAAACTCAGTGGGTAGGAAAAAGAGAACAACAAGCTAGCAGGGTCTTTGTTCACCTAGATTCTTTCATATTCTGGGTTTTAAAGAAGTTACTATACATTAACTGTCCTGAAGATGTCCTTAGATAAGTTCCTCGCCCACTTGCCCCACTTCTGTATCCATCCACATTTAAATTCTCTAAAAGGCCATGAATCTTACTTCATTCCATGACTTTTCCCCTAGGGAAATNNNNNNNNNNNNNNNNTGTATGTATGNNNNNNNNNNNNNNNT SEQUENCE_INCLUDED_REGION=11,359 SEQUENCE_TARGET=328,16 SEQUENCE_TARGET=354,15 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=48.RHAP33FD12.seq SEQUENCE_TEMPLATE=CNTTTTANGGGGTTGGTGGTTCATCTGAAAGTGTTGAGCACCTTTTCTACTCCTGCTTATATATTTTTATCAAAAGAAGAAGAAGAAGGAGAAGAAAAATGAAATAGCAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGANTGATGTGTTTGTACGGNNAGNATGAGNTTTCATNGTTCTCTTNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGAAGGATGCTNNTCCTATACANTGAGAGAATATCCTTTATTACNCTGNCNTTAGTTTGTTACATGGAAGAATTTTTGCCTGCNGGTTTAGCCCTCAGGCAAGNCGNG SEQUENCE_INCLUDED_REGION=0,361 SEQUENCE_TARGET=112,58 SEQUENCE_TARGET=222,28 P3_COMMENT=CL is GGGTTGGTGGTTCATCTGAA P3_COMMENT=CR is GCAGGCAAAAATTCTTCCAT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.596137 PRIMER_LEFT_0_PENALTY=0.758218 PRIMER_RIGHT_0_PENALTY=0.837920 PRIMER_LEFT_0_SEQUENCE=GGGTTGGTGGTTCATCTGAA PRIMER_RIGHT_0_SEQUENCE=GCAGGCAAAAATTCTTCCAT PRIMER_LEFT_0=9,20 PRIMER_RIGHT_0=335,20 PRIMER_LEFT_0_TM=60.758 PRIMER_RIGHT_0_TM=59.162 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=327 PRIMER_PAIR_1_PENALTY=1.936516 PRIMER_LEFT_1_PENALTY=0.758218 PRIMER_RIGHT_1_PENALTY=1.178298 PRIMER_LEFT_1_SEQUENCE=GGGTTGGTGGTTCATCTGAA PRIMER_RIGHT_1_SEQUENCE=CTTGCCTGAGGGCTAAACC PRIMER_LEFT_1=9,20 PRIMER_RIGHT_1=355,19 PRIMER_LEFT_1_TM=60.758 PRIMER_RIGHT_1_TM=59.822 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=347 PRIMER_PAIR_2_PENALTY=2.398813 PRIMER_LEFT_2_PENALTY=1.560893 PRIMER_RIGHT_2_PENALTY=0.837920 PRIMER_LEFT_2_SEQUENCE=GGTTGGTGGTTCATCTGAAAG PRIMER_RIGHT_2_SEQUENCE=GCAGGCAAAAATTCTTCCAT PRIMER_LEFT_2=10,21 PRIMER_RIGHT_2=335,20 PRIMER_LEFT_2_TM=59.439 PRIMER_RIGHT_2_TM=59.162 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=326 PRIMER_PAIR_3_PENALTY=2.450989 PRIMER_LEFT_3_PENALTY=1.613069 PRIMER_RIGHT_3_PENALTY=0.837920 PRIMER_LEFT_3_SEQUENCE=GGTTGGTGGTTCATCTGAAA PRIMER_RIGHT_3_SEQUENCE=GCAGGCAAAAATTCTTCCAT PRIMER_LEFT_3=10,20 PRIMER_RIGHT_3=335,20 PRIMER_LEFT_3_TM=58.387 PRIMER_RIGHT_3_TM=59.162 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=326 PRIMER_PAIR_4_PENALTY=2.535567 PRIMER_LEFT_4_PENALTY=1.697647 PRIMER_RIGHT_4_PENALTY=0.837920 PRIMER_LEFT_4_SEQUENCE=GGGTTGGTGGTTCATCTGA PRIMER_RIGHT_4_SEQUENCE=GCAGGCAAAAATTCTTCCAT PRIMER_LEFT_4=9,19 PRIMER_RIGHT_4=335,20 PRIMER_LEFT_4_TM=59.302 PRIMER_RIGHT_4_TM=59.162 PRIMER_LEFT_4_GC_PERCENT=52.632 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=327 = SEQUENCE_ID=48.RHAP34FD12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNACCTAGGTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACAACTGTTAACGTAGGAAGCTTCGGTGATCTTCCTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=10,120 SEQUENCE_TARGET=21,33 SEQUENCE_TARGET=57,32 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=48.RHAP34FH12.seq SEQUENCE_TEMPLATE=CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTACAANAAGTCAGGGAGNGGGAGGAGTTCTGNACAGATGGCTTACTGAACCACAATGTGAAAAAGCATGACTTAATGGTGNTCATTTGAGTTTATCCCTTNTCAATACCAACGANAGGGAAGAANTTNAATTGACCAGACACACCGACNNTGAGGGGTCNNAATTGNAACACCNTAGAGAGAGACAAAGAAATGATACTNGNACAAGNTNTGNAAGAGAGNCACTATGTATGNAAGAAGTGNGAGAGNANACATTTCTCCNNGNCACGGTAATAAGCTNGAGAGAGNTNTANTCAACNAAGGAATACATNGGACATAAAAGNGNAAGAAAAGAATCNCATNGTCAACATTGNANTACACAATACACTGNAAGNAGCAAAGNTGA SEQUENCE_INCLUDED_REGION=0,420 SEQUENCE_TARGET=1,34 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=03.RHAP61FA3.seq SEQUENCE_TEMPLATE=GTCATTGTATTNATCACTACANCAATAAAAANGAAANNGCGACAACTCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTNTGTATGTCNNTTTANNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNTNGNGTGTGNCCCCTGTATATATGTGCTTNNNNGTGTGANNGCNCGNNTATNNNTNTNNGATNCCNGNTGGNNNNNNNNNNNNNNNNGTCGTNNANNNANNNNNTGNNANNTNAAGGGNNTCNACCCCNANANCANNTCTNTAGGCNCCANNGAANNTNGAAATNNNNNANNNANNGTCATTANCCNGTNNNCTGNCTCCAAANNNNTANCCGNNCAANANNNCCCANAATCANNNCAAGNCNGAANTANNNANCCGCAAANNNNCNGGNNNCNNAANNNTGTCNNNNNNANNCACNNTTANNNCGCNCCCNCNNNTCACCNGCCAGNNTTGCANCNNNGNANAT SEQUENCE_INCLUDED_REGION=0,485 SEQUENCE_TARGET=52,30 SEQUENCE_TARGET=102,16 SEQUENCE_TARGET=119,20 SEQUENCE_TARGET=213,14 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=04.RHAP61FB1.seq SEQUENCE_TEMPLATE=AGACCCCCTTGTTGATGTGTTTGTGCACAGATCNGTATCTGTGAACACTGACAGAAGGGAGGAAGGAACTGAGTGTCTTGAAAGATATGAGGGAATATGGCTATCTCTGTNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNTNNNNTTTTATATACATCTGCATCTTTNCATAGACACCCCATGAAAANGNACCTTGCAAATTCNTTATGGGNNANGTNTGNGTTTGTCAAAATNGGANTNNTCTTGNNAGCTAACTTCCGGNTNNNTAGAATGTATTATCCNCTNTATGTTNGACACTCTNTTGTTGNTGTGTTTCNTAAATAGGGGTANGCGCTCTGNNTTCTGTANNCNTNGGTCNTAGCTGTTTCCTGTTGGNAAATTTNTTNTGCGCNCNTATATTCCNCNNNACATTGTGNGGCGCANNCNTANTGTTTAAACNCNNGGGTCCCNNGTGAGGGCGCGTTTCGCNCNT SEQUENCE_INCLUDED_REGION=0,538 SEQUENCE_TARGET=113,34 SEQUENCE_TARGET=148,54 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=04.RHAP61FB7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNTGTAGTGTAAGTACCANTTATAACCCTAAGCCAGNTTTGAGAGGCAATAAGTCAACACTTGGATGAGAAAGTGGNACCAGGCCTTTGGTTTGGTCAACAACCTTTTGAGGCAACCATATTATCACCCNTTATTTCACAGATGAAGAAGTTGCACAAAGAAAGTAAAGCAGTATCATATATACACACACATATATGTATACCTACCCATCATATATACATACATATGTTACATATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCGTAAGCTCCNNAGCCNGGCCTGCANNTGGGGGGATCCNNTGNCAGTGCACCGCACANTGTATCACTGCGNGAAGATCCGNCAGGGNNTCTAGTTTCNNCAACNGCNTNNCNGAGGGATGCNGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,401 SEQUENCE_TARGET=257,36 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=06.RHAP61FB3.seq SEQUENCE_TEMPLATE=TCAAGATGGTGCAGAGGTAAAGGCAGTTGCTGCCAAGATTGAANACCTGACTTCAATCACTNNAACCTACATGTTGGAGGGAANAACGNACTCCAATTTTTCCTCTGACCTTCAACAGGCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNTGCTTAAATTCAAACANTGANATTGTNATTGGCCAAAATGNTTNATTTCCGCTCACANTTTTANAGCATTCNGGGGTCGGNGGGCNTCCTCCNTNGGNATTTGNNCCNGGGNNANCNACNTTGTCGANAATAAGAACCATGNNCGCGCCAANGANGNNNANNGAGGGTTNCNNGCTCGNNTTNGTANNNNANGGTCANANNNGTTTCNTGGGNNNANNTGGTNTCNGGNNNNNAAATCNNNNCAANNTTCNNGGCCNTGGAGGNATTAAGGNGNNNGCCTTGGGGGGGNNANNGNGTNAGT SEQUENCE_INCLUDED_REGION=0,528 SEQUENCE_TARGET=124,55 SEQUENCE_TARGET=181,40 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=06.RHAP61FB9.seq SEQUENCE_TEMPLATE=CNTCCNCCNNCCNCCNCTCNCGCCNNNNNNNNNNNNNNNNNNCNNNNNCNNNNNNNNNNNCNNNCNGANTNTGGTGNAATNTGNNGNTCNCNTNGGGGATCNNNAANCNCCCNNANNNNNNNNNNGNNGNNGANNNGTNCNANNTTNNNNNTTGGNGNTNAANNNNNAAATNNNTGNTNNGNAGNANGNGGNNTAAGNNNNCNNTANANGANNNNCNNNANNNNGNNNNNAGTANNNNATNGGNNNNGTTTNGGANGNNGNNATNGNNNAGANNAANNGNGNTGNGACTAGGGNGATNNGGANAAGANGGNNANNGNAAGNGGNGTNNNGGNNTNNTGGNANNTGTNNTGNNNGANNGGNNGNGGNNTAGGANNGNNTGTNNNTNTGGTNNANAAGTNNGNNNTGNNNNTGNNNNNNGGGTTTNNGNANGNNNNNNNNNTTAANNGGNNNNNNANANNTANNTGNNAAAAGGNNNNNNGGGANNNGNNNTAAGNANNGGGNGTNNGGNNGNGGNNNNNTGTGNNNGNNGNTTGGNGNNTNGNAGNNNNGNNNNNNNANGNNNTGNNNAAANT SEQUENCE_INCLUDED_REGION=0,572 SEQUENCE_TARGET=24,16 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=07.RHAP61FC7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNTGGGCGTGGTCCCGAGACACTCCCCGCGGGGGCGNCCGGGGCGGGGCGCGGCACCAGGTGGAGGGCGCTGACCCAAGCAACGTCAGTCTGGAAACTGCGTTCCCTAGGCATCTTATCCCCCTAGACCTAGCAGCAGAAGTACCCCAATTCAGTCTCTCAATTCAGTGCTCTTGGAGGAGAGTGTACCCACTCAAGGANTNNCNCGGGCTGGCTGAGCGGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,267 SEQUENCE_TARGET=233,44 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=11.RHAP61FD8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTCAGAATTGTTGGGAAGACAGAGTGAAACCCATGGCCTGAGCAGTATCTAGTCTCGGTAANTAGCAAGAAGANAAGGCTAGAAAGGCTGAGGTCTAGATAGGGCTATCCTGGGCTAGACTGAGTGGGTGATCATGACCAACACCAAGGGACTCAGGTGAAGCCCTCTACAAAGGTCCAGACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGNGNGNNNNNNNNNNNNNNNGTCGTGNTGNGGTGTNNGNGTNNGNNNNNNNGGGTGTGNNGTGNNNNNNNGNNTNGGGGNNGNNNNNNNNGGNNNGNNGANNNAGNGGGNGGAGGNCTNTAGNGNANGNNGNNNNNNGGNNANAGNNNNNNNNNGGGTNNNNGNNGNNNANNNNGNNGGNNNANNGTGGGGTNNNGGGGNNAGNNGNNGGNNTNGNGNGAAGNGNNGGGGNNNTGNGNNANNGGGNTNGANNNGNGNGNNNGNGGNA SEQUENCE_INCLUDED_REGION=19,483 SEQUENCE_TARGET=203,28 SEQUENCE_TARGET=240,15 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=12.RHAP61FD9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTGTTTTGGAAATTNGAGGAACAAAATATAAAANNCATTAAAAAATTCCCATTTCTGAAAATTATCACTGGAGTTANGGACAATTATGAGCCACACATTGTGGCTCATGATAAGAATNNNCAGAGGGAAANTANGATCTNNNTGNGAAAGAGGGACTNTGNAGNGNCNCNCNNANNGNCNNNAANNNNNCGNTNNGNNNCCNNCTCNNTCACTGNNNNNNNNNNNNNNNNCTNNTCNNTTTNTAGAATCGNGNNGNTGGTCTGAAAACNTNNNNGNNCACNNNNTCTTGGTTANCNTCANTTNTAAAATGNNTGANGNTGNTAGTAGGGNNGCNGCGTGNNNNCCNCCNTCCCAGGNNANCCNGNGCCCCATGNNNCNTCAAANCAANTNNNTGGANCCNCAANGTGNNANGGCCCNNCNATCCNNGNNNNTCNTGNNNNNGNGNTCATTGCAAANTNAGNGNNNNNNNNCNNNANTG SEQUENCE_INCLUDED_REGION=21,478 SEQUENCE_TARGET=236,14 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=13.RHAP61FE7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTTGAACATTTACTACATTTCCTGGGCTCACCANTTTGTNAATTTGCCTTTTTCTGTTCCATATGCANTTTTACCATCATCAGGTATAATGAATGGGTCTTAGCAGATTCCACTTGGGTGNTCTANTGAATTCTNNNNNNNNNNNNNNNNNNNNNNNNNGNGNNNCCNCCCNCNNNNNCNNTGNNNNNNNNNNNTGNNNNNGNCNCNNNNNCGGNNNGNGGGNNGCTGNGGAANNNNGGNNGNGNNGGGGNGNNNNGGGGNNNGNNNNGGGTGGGGGNGNNGCAGTGNNNNGGGGNNGNGGNGNGNNTGNGGNGTGGNNGNNNGGNNGTGNNNGGGNGGNGGGGGTTGGGNGNNGNNGTNNNAANNGGGGGNNNNGTNGGGGGNNGNNNNGNNNNNNNNNGGGANNNGGGGGNNTTNNNNGGGNGAGNNNGANNNGNNNNNGGGNNNANNNNNGAGGGNNGGNANNNGTGNGGGNNAGGGNNNGTTNNGGNNTTAAA SEQUENCE_INCLUDED_REGION=21,497 SEQUENCE_TARGET=155,24 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=14.RHAP61FE8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTAGGACTCTGAAGTATATTCAGGTACTTGTGATGCTGAGGGAGCCTGAAGGCAGCACCGAGTTTTGGTGTGCTGATAGAAACCAAAGATAGTTATTTGTCCCTTCTGCCTTTTAGGATCTGGGGAAACGGAGTTTCTTTTGGACCTGACAGCAGTTTGCATGNGNGTCNGNGATGTATTTTGATCACGTTCACCGTTATCTTGTCCCCCTCCCACCCCTACAGACCTTCTACCTCTGTGTGTGCATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCANTGGTGTTACAGGAAAATGAGAGAGGTTCGTTACACTGGGGCACCTTGCCAGTGGCGTACATCACTGACAAAGTTTCTNTCCCTCCCAGCAAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,401 SEQUENCE_TARGET=268,50 P3_COMMENT=CL is ACCGTTATCTTGTCCCCCTC P3_COMMENT=CR is CCCCAGTGTAACGAACCTCT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.128597 PRIMER_LEFT_0_PENALTY=0.190281 PRIMER_RIGHT_0_PENALTY=0.938316 PRIMER_LEFT_0_SEQUENCE=ACCGTTATCTTGTCCCCCTC PRIMER_RIGHT_0_SEQUENCE=CCCCAGTGTAACGAACCTCT PRIMER_LEFT_0=210,20 PRIMER_RIGHT_0=362,20 PRIMER_LEFT_0_TM=60.190 PRIMER_RIGHT_0_TM=59.062 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=153 PRIMER_PAIR_1_PENALTY=1.147975 PRIMER_LEFT_1_PENALTY=0.209659 PRIMER_RIGHT_1_PENALTY=0.938316 PRIMER_LEFT_1_SEQUENCE=TTCACCGTTATCTTGTCCCC PRIMER_RIGHT_1_SEQUENCE=CCCCAGTGTAACGAACCTCT PRIMER_LEFT_1=207,20 PRIMER_RIGHT_1=362,20 PRIMER_LEFT_1_TM=59.790 PRIMER_RIGHT_1_TM=59.062 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=10.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=156 PRIMER_PAIR_2_PENALTY=1.208793 PRIMER_LEFT_2_PENALTY=0.190281 PRIMER_RIGHT_2_PENALTY=1.018512 PRIMER_LEFT_2_SEQUENCE=ACCGTTATCTTGTCCCCCTC PRIMER_RIGHT_2_SEQUENCE=GCCCCAGTGTAACGAACCT PRIMER_LEFT_2=210,20 PRIMER_RIGHT_2=363,19 PRIMER_LEFT_2_TM=60.190 PRIMER_RIGHT_2_TM=59.981 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=154 PRIMER_PAIR_3_PENALTY=1.228171 PRIMER_LEFT_3_PENALTY=0.209659 PRIMER_RIGHT_3_PENALTY=1.018512 PRIMER_LEFT_3_SEQUENCE=TTCACCGTTATCTTGTCCCC PRIMER_RIGHT_3_SEQUENCE=GCCCCAGTGTAACGAACCT PRIMER_LEFT_3=207,20 PRIMER_RIGHT_3=363,19 PRIMER_LEFT_3_TM=59.790 PRIMER_RIGHT_3_TM=59.981 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=57.895 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=157 PRIMER_PAIR_4_PENALTY=1.300852 PRIMER_LEFT_4_PENALTY=0.362536 PRIMER_RIGHT_4_PENALTY=0.938316 PRIMER_LEFT_4_SEQUENCE=CCCACCCCTACAGACCTTCT PRIMER_RIGHT_4_SEQUENCE=CCCCAGTGTAACGAACCTCT PRIMER_LEFT_4=229,20 PRIMER_RIGHT_4=362,20 PRIMER_LEFT_4_TM=60.363 PRIMER_RIGHT_4_TM=59.062 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=134 = SEQUENCE_ID=16.RHAP61FF1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNTGTCATTTTCTTTTTAATCTCTGTTTTTCTGCCATTCTAGGTCAGCTGAAGATGAAGGTCTCCTCTACTGCTCTGGAGCCTNTNCACGGAGACCCTAATTTCATGCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTTCCNTTGAAATAATGGAAGATATTAAAGTATAAAAATAAGAGAGTTGTACCATCCATACCCATTCTGAAAATGAAGCCAAATGTAATCTTATCACTAAAGAGCATGTTATATNTGAACAGAGACATTTTATACACTATAAGATTAGATCTCAAANTNNTTATNTACCTGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,327 SEQUENCE_TARGET=120,44 P3_COMMENT=CL is TCCTCTACTGCTCTGGAGCC P3_COMMENT=CR is GGCTTCATTTTCAGAATGGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.792069 PRIMER_LEFT_0_PENALTY=0.296722 PRIMER_RIGHT_0_PENALTY=0.495347 PRIMER_LEFT_0_SEQUENCE=TCCTCTACTGCTCTGGAGCC PRIMER_RIGHT_0_SEQUENCE=GGCTTCATTTTCAGAATGGG PRIMER_LEFT_0=71,20 PRIMER_RIGHT_0=244,20 PRIMER_LEFT_0_TM=59.703 PRIMER_RIGHT_0_TM=59.505 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=9.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 PRIMER_PAIR_1_PENALTY=0.967399 PRIMER_LEFT_1_PENALTY=0.472052 PRIMER_RIGHT_1_PENALTY=0.495347 PRIMER_LEFT_1_SEQUENCE=CGGAGACCCTAATTTCATGC PRIMER_RIGHT_1_SEQUENCE=GGCTTCATTTTCAGAATGGG PRIMER_LEFT_1=97,20 PRIMER_RIGHT_1=244,20 PRIMER_LEFT_1_TM=59.528 PRIMER_RIGHT_1_TM=59.505 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=8.4000 PRIMER_RIGHT_1_END_STABILITY=9.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=148 PRIMER_PAIR_2_PENALTY=1.532033 PRIMER_LEFT_2_PENALTY=0.472052 PRIMER_RIGHT_2_PENALTY=1.059980 PRIMER_LEFT_2_SEQUENCE=CGGAGACCCTAATTTCATGC PRIMER_RIGHT_2_SEQUENCE=TTGGCTTCATTTTCAGAATGG PRIMER_LEFT_2=97,20 PRIMER_RIGHT_2=246,21 PRIMER_LEFT_2_TM=59.528 PRIMER_RIGHT_2_TM=60.060 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=8.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=150 PRIMER_PAIR_3_PENALTY=1.601557 PRIMER_LEFT_3_PENALTY=0.296722 PRIMER_RIGHT_3_PENALTY=1.304835 PRIMER_LEFT_3_SEQUENCE=TCCTCTACTGCTCTGGAGCC PRIMER_RIGHT_3_SEQUENCE=TGGCTTCATTTTCAGAATGG PRIMER_LEFT_3=71,20 PRIMER_RIGHT_3=245,20 PRIMER_LEFT_3_TM=59.703 PRIMER_RIGHT_3_TM=58.695 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=8.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=175 PRIMER_PAIR_4_PENALTY=1.609992 PRIMER_LEFT_4_PENALTY=0.296722 PRIMER_RIGHT_4_PENALTY=1.313269 PRIMER_LEFT_4_SEQUENCE=TCCTCTACTGCTCTGGAGCC PRIMER_RIGHT_4_SEQUENCE=GGCTTCATTTTCAGAATGGGT PRIMER_LEFT_4=71,20 PRIMER_RIGHT_4=244,21 PRIMER_LEFT_4_TM=59.703 PRIMER_RIGHT_4_TM=60.313 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=174 = SEQUENCE_ID=18.RHAP61FF3.seq SEQUENCE_TEMPLATE=GTAGCCGACACCATCCCCCGACACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACCAAACAAACAAACAAACAAATCAGCAAAACGTTTAGTCAATTCTGCTTTTCTTACTGGAAAGCCCAAAGCCTGTGTCTTAGCATGGCTGATTATCCTCTCTTCTGGGCAAGATTTCCTCAGTGCCAGTTTTATGTTTGCAAGGTCCCATCTATGCCAAGCATCATGTTAGCCCTAATATTTCTGATGCATTCCAAGCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,271 SEQUENCE_TARGET=34,34 P3_COMMENT=CL is TCCCCCGACACATACATACA P3_COMMENT=CR is TAAGACACAGGCTTTGGGCT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.476466 PRIMER_LEFT_0_PENALTY=0.351810 PRIMER_RIGHT_0_PENALTY=0.124655 PRIMER_LEFT_0_SEQUENCE=TCCCCCGACACATACATACA PRIMER_RIGHT_0_SEQUENCE=TAAGACACAGGCTTTGGGCT PRIMER_LEFT_0=13,20 PRIMER_RIGHT_0=151,20 PRIMER_LEFT_0_TM=59.648 PRIMER_RIGHT_0_TM=59.875 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=5.6000 PRIMER_RIGHT_0_END_STABILITY=10.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=139 PRIMER_PAIR_1_PENALTY=0.476466 PRIMER_LEFT_1_PENALTY=0.351810 PRIMER_RIGHT_1_PENALTY=0.124655 PRIMER_LEFT_1_SEQUENCE=TCCCCCGACACATACATACA PRIMER_RIGHT_1_SEQUENCE=CTAAGACACAGGCTTTGGGC PRIMER_LEFT_1=13,20 PRIMER_RIGHT_1=152,20 PRIMER_LEFT_1_TM=59.648 PRIMER_RIGHT_1_TM=59.875 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=5.6000 PRIMER_RIGHT_1_END_STABILITY=11.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=140 PRIMER_PAIR_2_PENALTY=0.476466 PRIMER_LEFT_2_PENALTY=0.351810 PRIMER_RIGHT_2_PENALTY=0.124655 PRIMER_LEFT_2_SEQUENCE=TCCCCCGACACATACATACA PRIMER_RIGHT_2_SEQUENCE=GCTAAGACACAGGCTTTGGG PRIMER_LEFT_2=13,20 PRIMER_RIGHT_2=153,20 PRIMER_LEFT_2_TM=59.648 PRIMER_RIGHT_2_TM=59.875 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=5.6000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=141 PRIMER_PAIR_3_PENALTY=0.779333 PRIMER_LEFT_3_PENALTY=0.351810 PRIMER_RIGHT_3_PENALTY=0.427522 PRIMER_LEFT_3_SEQUENCE=TCCCCCGACACATACATACA PRIMER_RIGHT_3_SEQUENCE=GCCATGCTAAGACACAGGCT PRIMER_LEFT_3=13,20 PRIMER_RIGHT_3=158,20 PRIMER_LEFT_3_TM=59.648 PRIMER_RIGHT_3_TM=60.428 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=5.6000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=146 PRIMER_PAIR_4_PENALTY=0.779333 PRIMER_LEFT_4_PENALTY=0.351810 PRIMER_RIGHT_4_PENALTY=0.427522 PRIMER_LEFT_4_SEQUENCE=TCCCCCGACACATACATACA PRIMER_RIGHT_4_SEQUENCE=AGCCATGCTAAGACACAGGC PRIMER_LEFT_4=13,20 PRIMER_RIGHT_4=159,20 PRIMER_LEFT_4_TM=59.648 PRIMER_RIGHT_4_TM=60.428 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=5.6000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=147 = SEQUENCE_ID=19.RHAP61FG1.seq SEQUENCE_TEMPLATE=NNACNNACTTANTCCNNAAAGNANNACNNNNNNNNNNNNNNNNTGNCGNNNNNNNNNNNNNNNNNNNACCCATNNAGNCATACATACNGTCACATGTGTGTATANACANCNGNAGTCAACCNNGCNTCTTNTTCCNCAATTANANCNCCTNAANANNANNGNNNNNTGATAGGNCNATTACNTCNCCACTNACCTGNGTNNNNGCGGNCTCTNNANTTCGGGGNATCATTNNCTTGAACGGCNCNCCNNTGNTNAANNNTTTGGCNGNNGGNNGNTTNNCACNNANGATGNGTNNNNNGGNNNGCATNNNAGNTGTNAAGGCNTCGGGNGGGNNNGTATGGGAGGTGNGGNNNGTCNANNGGANGGNNTCGNNGGNGNNCANGTTCCNGNNGNNNGNNNNCNNGAANTNTTNNNGNNNNGGNNGGNGNGANGNNAGGATGNNAGNTCNNNNNGANNGTGNGNTTNGNNGNNTANGNGNNNCTGGGGGGGGNANAANNTTNNNGGGTGGTGTCGNTGGNNTGGNTGGTTTGNNTGCNTAGNNT SEQUENCE_INCLUDED_REGION=0,542 SEQUENCE_TARGET=29,14 SEQUENCE_TARGET=49,18 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=20.RHAP61FG2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNTAACAACTGAGACAGAAGAAAATGTCAGAAATGAAAAAAATAAAAAAAGCAGAAAACTNACTNATCAACCGTAAAAACAAACCAACCAAAATGTAAATTAAAAATCTAAAACACTTATACGTGACATTCAGAAATGTCTGTCAATGACTGAGAATTATTCACATCTAAACAATGAAAGAACACACAGTGCAATTATATCTGTATTAAATGTTTCCAGTTTGGGAGATATGCATTGAAGTACCAATATTGATCAAGATCTTTCTTGCGGGTGTGTGAGTATTAAATATGAATGTGNNAACAGTATGTTAACATCTGAGAGCATNCTCCATCATTCTGTCACCTCAGGTTTCATAGAAGAAATNTCCTATTGNCATGTTGTAGACTNTTTGGGAAAGNGNTANNNGCACTTTNAATCCCAGNNTCACCCTCTCAGGTCNTGGGATCTGNNCCACNGCCGTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGAAGT SEQUENCE_INCLUDED_REGION=12,509 SEQUENCE_TARGET=475,37 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=21.RHAP61FG9.seq SEQUENCE_TEMPLATE=AATGANTTAGAGGACCCCTTCCTCACAGGTCCAGAAGGACACTGAAGAANAGGTTAGCCAGATATGCTAAGGCTCTTGTCACTAGTCAGTTCTGAATTGCTTCCCTTTAATGTCTTGTCCGGGAAGGGACTGAGAATCCANTCANACATGTCTACCNTGNGNCTCNGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTNTCTCTCTCCTCAACCCAACCCAAACCACATTTCCCCCCCACACNNTCCCTCATATCNCCTTGNTTGNNATTCCCCCCCCCCCCCNACTNANANNTACNCNTNNNTTNTTTNAANNANAAAACCNCTNNNAAAAAAAACCNNNNGTAGNNNNNANANCNAAAATTTNTAAANANGNNNNNNG SEQUENCE_INCLUDED_REGION=0,479 SEQUENCE_TARGET=169,44 SEQUENCE_TARGET=215,40 SEQUENCE_TARGET=262,30 P3_COMMENT=CL is TAATGTCTTGTCCGGGAAGG P3_COMMENT=CR is GGTTGGGTTGAGGAGAGAGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.846305 PRIMER_LEFT_0_PENALTY=0.074208 PRIMER_RIGHT_0_PENALTY=0.772097 PRIMER_LEFT_0_SEQUENCE=TAATGTCTTGTCCGGGAAGG PRIMER_RIGHT_0_SEQUENCE=GGTTGGGTTGAGGAGAGAGA PRIMER_LEFT_0=107,20 PRIMER_RIGHT_0=317,20 PRIMER_LEFT_0_TM=59.926 PRIMER_RIGHT_0_TM=59.228 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=1.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=211 PRIMER_PAIR_1_PENALTY=1.001278 PRIMER_LEFT_1_PENALTY=0.229181 PRIMER_RIGHT_1_PENALTY=0.772097 PRIMER_LEFT_1_SEQUENCE=TTCCTCACAGGTCCAGAAGG PRIMER_RIGHT_1_SEQUENCE=GGTTGGGTTGAGGAGAGAGA PRIMER_LEFT_1=18,20 PRIMER_RIGHT_1=317,20 PRIMER_LEFT_1_TM=60.229 PRIMER_RIGHT_1_TM=59.228 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=1.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=300 PRIMER_PAIR_2_PENALTY=1.001278 PRIMER_LEFT_2_PENALTY=0.229181 PRIMER_RIGHT_2_PENALTY=0.772097 PRIMER_LEFT_2_SEQUENCE=CCTTCCTCACAGGTCCAGAA PRIMER_RIGHT_2_SEQUENCE=GGTTGGGTTGAGGAGAGAGA PRIMER_LEFT_2=16,20 PRIMER_RIGHT_2=317,20 PRIMER_LEFT_2_TM=60.229 PRIMER_RIGHT_2_TM=59.228 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=1.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=302 PRIMER_PAIR_3_PENALTY=1.090008 PRIMER_LEFT_3_PENALTY=0.317911 PRIMER_RIGHT_3_PENALTY=0.772097 PRIMER_LEFT_3_SEQUENCE=CCTCACAGGTCCAGAAGGAC PRIMER_RIGHT_3_SEQUENCE=GGTTGGGTTGAGGAGAGAGA PRIMER_LEFT_3=20,20 PRIMER_RIGHT_3=317,20 PRIMER_LEFT_3_TM=59.682 PRIMER_RIGHT_3_TM=59.228 PRIMER_LEFT_3_GC_PERCENT=60.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=1.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=298 PRIMER_PAIR_4_PENALTY=1.094878 PRIMER_LEFT_4_PENALTY=0.074208 PRIMER_RIGHT_4_PENALTY=1.020670 PRIMER_LEFT_4_SEQUENCE=TAATGTCTTGTCCGGGAAGG PRIMER_RIGHT_4_SEQUENCE=GGGTTGGGTTGAGGAGAGAG PRIMER_LEFT_4=107,20 PRIMER_RIGHT_4=318,20 PRIMER_LEFT_4_TM=59.926 PRIMER_RIGHT_4_TM=61.021 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=1.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=212 = SEQUENCE_ID=24.RHAP61FH3.seq SEQUENCE_TEMPLATE=TAGAAATGNGACTGGTGAGATTTNANATTGTCCAANCATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATTTCAAACATTTCTGTGTGTGTTTCTCCAGATCTTCCTCTTTCTCTTCCTATACACATGAGAAAAGTANAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGCGGCGCGACACACACANATGGACAAATTGGNAAGANAGAAAGGATTNANTNNTTTAGACAACAGGTGTNNGAGATCNGGATTTTTCTCNTANANNTGTNGNCTGTTGNNAGGTNNCCAGGTGANTAGGGATNGGTGNNGANTTANNGGGGGGAGGTGCGNGGTNTNNCAGNNNNTTNATGGNTGGNGNTNGGTGAANNANANNTTCTCAGGNNNANNGNNGGGGGACGNATGTGGTTGGNNNNNNANNNNNANNNNNNTNTTGCNGNNGGNNNNNNGGNGNNTGNNNNTTTGNNTGANGGNNGGGGNGNNCNNCAAT SEQUENCE_INCLUDED_REGION=0,529 SEQUENCE_TARGET=43,42 SEQUENCE_TARGET=162,44 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=26.RHAP61FA11.seq SEQUENCE_TEMPLATE=TNCCANCNTCCNATGCACCCACNCNGCCCCAGGGAANNACCGNNNACNCCCGNCTTCCNGNNCCCCATCNTCTGGCACGTNNACCCGNGNANATGCNNNNNNNNNNNNNNNNNCCCNNACNNACACACTNNAAATCNNTANAANANGNNTTNNATTCAGATAATACTATNNNCCNTGNTTNACACTTNCNNNNNCGNCATANTGCANATAACATGTTATGTAGGCATNTTGTCTGATGNAGNGGNACATGGCCCAGGCCCNAGTTNGGATCCTTAGNCACGNNTNANAGGNNNTGGGNATGNTNATTCNGCCCACAGGTNTTGGGNTCGTCANNANGGGGTACAGANNGNGANTTNGCNANNATGNNNATAAGGATGGNNNCNTGTGCGNNNTTGNTANNGNNANNNNTTTNCCAGGNNNANTANGGNGGGGGNNNNNTNNAGTGTNANT SEQUENCE_INCLUDED_REGION=0,450 SEQUENCE_TARGET=99,14 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=26.RHAP61FA5.seq SEQUENCE_TEMPLATE=CTGTGTGAATTTCTAAGTCTTACCTTCAGAAGCAGTACCAGGTNGACATGAAAATACTGTCACTCTCTCAAATCATGTAATATTTCTGTTTGACTTGACATATGCAGAGAGTCTCTGTTTCAGCATAAGACATGCTAGCAGGCTTAAAAATACTGTTTCTGGCTGCTAAATCCTGAATCTACAAATGTGGGATCAATGATGTGAAAAGAGAAACTAAATGATGTGTGGAAGTTTATCAACTCACAGTGACAAGAAGCAGACTGCAGCAAGGTACCACACATTCAGGAAGTGAATGACTCCTTAAGTNATTCTCCTATAGAAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCNTGNGATNTATNNGCCATGAATGTTTNTGNNTCTAGATACAAGAAAAGGGNTGGGTATTTNTGNGGCTCCNACAAGGGGCTTANTCACCAANTTTCTTTCNNGNNGAGGGGTTNCGGCNCGNATTT SEQUENCE_INCLUDED_REGION=0,508 SEQUENCE_TARGET=324,56 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=27.RHAP61FA12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTCTGATGGAGCCTGTAGTCTAGCAGTATGTTTTCCTGAGACAAAAAAAGACACAGAAAAANATTGGAAGATTTAGCAATATGGAAGGATGCTGAAACCACAGGTATTCTTGGAATTCTTTCACTTACCTTAGTATGCCCCTTGTGNGGATTGAATGNNNTCCTGNAATACAGGCTGTCCTAGATTACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAGGTTTTGGAGATGAAAAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,258 SEQUENCE_TARGET=213,42 P3_COMMENT=CL is AAGGATGCTGAAACCACAGG P3_COMMENT=CR is TCCTTTTCATCTCCAAAACCTT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.000620 PRIMER_LEFT_0_PENALTY=0.111077 PRIMER_RIGHT_0_PENALTY=2.889543 PRIMER_LEFT_0_SEQUENCE=AAGGATGCTGAAACCACAGG PRIMER_RIGHT_0_SEQUENCE=TCCTTTTCATCTCCAAAACCTT PRIMER_LEFT_0=105,20 PRIMER_RIGHT_0=278,22 PRIMER_LEFT_0_TM=60.111 PRIMER_RIGHT_0_TM=59.110 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=36.364 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 PRIMER_PAIR_1_PENALTY=3.310315 PRIMER_LEFT_1_PENALTY=0.420772 PRIMER_RIGHT_1_PENALTY=2.889543 PRIMER_LEFT_1_SEQUENCE=AGGATGCTGAAACCACAGGT PRIMER_RIGHT_1_SEQUENCE=TCCTTTTCATCTCCAAAACCTT PRIMER_LEFT_1=106,20 PRIMER_RIGHT_1=278,22 PRIMER_LEFT_1_TM=59.579 PRIMER_RIGHT_1_TM=59.110 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=36.364 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=173 PRIMER_PAIR_2_PENALTY=3.338080 PRIMER_LEFT_2_PENALTY=0.111077 PRIMER_RIGHT_2_PENALTY=3.227003 PRIMER_LEFT_2_SEQUENCE=AAGGATGCTGAAACCACAGG PRIMER_RIGHT_2_SEQUENCE=TCCTTTTCATCTCCAAAACCT PRIMER_LEFT_2=105,20 PRIMER_RIGHT_2=278,21 PRIMER_LEFT_2_TM=60.111 PRIMER_RIGHT_2_TM=57.773 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=174 PRIMER_PAIR_3_PENALTY=3.431150 PRIMER_LEFT_3_PENALTY=0.111077 PRIMER_RIGHT_3_PENALTY=3.320073 PRIMER_LEFT_3_SEQUENCE=AAGGATGCTGAAACCACAGG PRIMER_RIGHT_3_SEQUENCE=CCTTTTCATCTCCAAAACCTTTC PRIMER_LEFT_3=105,20 PRIMER_RIGHT_3=277,23 PRIMER_LEFT_3_TM=60.111 PRIMER_RIGHT_3_TM=60.320 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=39.130 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=173 PRIMER_PAIR_4_PENALTY=3.431150 PRIMER_LEFT_4_PENALTY=0.111077 PRIMER_RIGHT_4_PENALTY=3.320073 PRIMER_LEFT_4_SEQUENCE=AAGGATGCTGAAACCACAGG PRIMER_RIGHT_4_SEQUENCE=TCCTTTTCATCTCCAAAACCTTT PRIMER_LEFT_4=105,20 PRIMER_RIGHT_4=278,23 PRIMER_LEFT_4_TM=60.111 PRIMER_RIGHT_4_TM=60.320 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=34.783 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=174 = SEQUENCE_ID=27.RHAP61FA6.seq SEQUENCE_TEMPLATE=GTAAAATGCGTANNAACCAGCACAANCAACACACTGTTTGAAGGCAGGAGAATTTTCTCTCATTCTGAGGAACATAAACTNTTTATTATGNACAGGCCTTTGGTAGAACCAGTATAATGTTTAATCAAATATTTGGGCATCTCATGCTCTGTGAGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCCCACAGACATCTAGAAGAATCTAGACAAGATAACAGAACATTNTCAACTAACTGAACCACCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,281 SEQUENCE_TARGET=158,56 P3_COMMENT=CL is CAGGCCTTTGGTAGAACCAG P3_COMMENT=CR is TTCTTCTAGATGTCTGTGGGATG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.940409 PRIMER_LEFT_0_PENALTY=0.271680 PRIMER_RIGHT_0_PENALTY=4.668729 PRIMER_LEFT_0_SEQUENCE=CAGGCCTTTGGTAGAACCAG PRIMER_RIGHT_0_SEQUENCE=TTCTTCTAGATGTCTGTGGGATG PRIMER_LEFT_0=92,20 PRIMER_RIGHT_0=236,23 PRIMER_LEFT_0_TM=59.728 PRIMER_RIGHT_0_TM=58.331 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=145 PRIMER_PAIR_1_PENALTY=5.076670 PRIMER_LEFT_1_PENALTY=0.407941 PRIMER_RIGHT_1_PENALTY=4.668729 PRIMER_LEFT_1_SEQUENCE=ACAGGCCTTTGGTAGAACCA PRIMER_RIGHT_1_SEQUENCE=TTCTTCTAGATGTCTGTGGGATG PRIMER_LEFT_1=91,20 PRIMER_RIGHT_1=236,23 PRIMER_LEFT_1_TM=59.592 PRIMER_RIGHT_1_TM=58.331 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=43.478 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_2_PENALTY=5.599600 PRIMER_LEFT_2_PENALTY=0.271680 PRIMER_RIGHT_2_PENALTY=5.327920 PRIMER_LEFT_2_SEQUENCE=CAGGCCTTTGGTAGAACCAG PRIMER_RIGHT_2_SEQUENCE=ATTCTTCTAGATGTCTGTGGGATG PRIMER_LEFT_2=92,20 PRIMER_RIGHT_2=237,24 PRIMER_LEFT_2_TM=59.728 PRIMER_RIGHT_2_TM=58.672 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=8.1000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=146 PRIMER_PAIR_3_PENALTY=5.645139 PRIMER_LEFT_3_PENALTY=0.271680 PRIMER_RIGHT_3_PENALTY=5.373459 PRIMER_LEFT_3_SEQUENCE=CAGGCCTTTGGTAGAACCAG PRIMER_RIGHT_3_SEQUENCE=GATTCTTCTAGATGTCTGTGGGATG PRIMER_LEFT_3=92,20 PRIMER_RIGHT_3=238,25 PRIMER_LEFT_3_TM=59.728 PRIMER_RIGHT_3_TM=60.373 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=44.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=147 PRIMER_PAIR_4_PENALTY=5.735861 PRIMER_LEFT_4_PENALTY=0.407941 PRIMER_RIGHT_4_PENALTY=5.327920 PRIMER_LEFT_4_SEQUENCE=ACAGGCCTTTGGTAGAACCA PRIMER_RIGHT_4_SEQUENCE=ATTCTTCTAGATGTCTGTGGGATG PRIMER_LEFT_4=91,20 PRIMER_RIGHT_4=237,24 PRIMER_LEFT_4_TM=59.592 PRIMER_RIGHT_4_TM=58.672 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=147 = SEQUENCE_ID=29.RHAP61FB11.seq SEQUENCE_TEMPLATE=GGCNNTATAGANNATCCCNTCNGGNCATNTCAANCCCACATNCTCTTANAANAACTTTGNAAAATGATGNNTGANNAAAANTTACTGCTNNNGNNNNNTCNACTTTGGNGNGNCTCTGNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATANGTGTCNATTTANNATGCANANCTNNNTCGGAGGTTGTGNTGNNCNCTCNAAAACTCNTTNTNNNTNCNTCCAGGGGNNGGGNNCNNCCGNNTNNNTCTCGNNCNTTTNNANATANGNANGTAANTCTGGNATCNTCCNANTNNACNNNTNAGNGAANNATGGGTNNNNGNAAGNGCTTTTNTNNNANGAGGGGGGGNGNNNNNGANNTGGAATGGGNANNNNGGGNNNAAAANNNGTTTTCCNTTGGNAANTTNNNNTCNNNNNNGANTNNNANAANAANATTNGAGNGGGAANCNNNAANNNTAAAGCNNGGGGNNCTT SEQUENCE_INCLUDED_REGION=0,490 SEQUENCE_TARGET=123,40 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=30.RHAP61FB12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTGCCTGAAAAAAGAAAATCACACTTTTTGATCTCTAGCCACCTAAAATGTTTGCTAAGTTTCACTTCCCAACACCAGGGGGTGTCTCTTCGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTGTCCTTAAGGTTCCATTAGTCCCAAACCTTTCTCCGTGTTTTTATGTACACTCTAAATCTTTCTCTGTCCCTCCAGAGGCAGGGATCAGCCTTATTTTCCTCGCTCTTTTTCATATAAGCACATAAATCTTGTATCCCCCCACTCCACCNNCAGAGAACCATGGGTCTAGAAAGTGCTTTTTATTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,332 SEQUENCE_TARGET=112,46 P3_COMMENT=CL is AACACCAGGGGGTGTCTCTT P3_COMMENT=CR is AATAAGGCTGATCCCTGCCT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.861541 PRIMER_LEFT_0_PENALTY=0.801345 PRIMER_RIGHT_0_PENALTY=0.060196 PRIMER_LEFT_0_SEQUENCE=AACACCAGGGGGTGTCTCTT PRIMER_RIGHT_0_SEQUENCE=AATAAGGCTGATCCCTGCCT PRIMER_LEFT_0=88,20 PRIMER_RIGHT_0=257,20 PRIMER_LEFT_0_TM=60.801 PRIMER_RIGHT_0_TM=60.060 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=9.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=170 PRIMER_PAIR_1_PENALTY=1.156250 PRIMER_LEFT_1_PENALTY=1.096054 PRIMER_RIGHT_1_PENALTY=0.060196 PRIMER_LEFT_1_SEQUENCE=CAGGGGGTGTCTCTTCGTT PRIMER_RIGHT_1_SEQUENCE=AATAAGGCTGATCCCTGCCT PRIMER_LEFT_1=93,19 PRIMER_RIGHT_1=257,20 PRIMER_LEFT_1_TM=60.096 PRIMER_RIGHT_1_TM=60.060 PRIMER_LEFT_1_GC_PERCENT=57.895 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.4000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=165 PRIMER_PAIR_2_PENALTY=1.220450 PRIMER_LEFT_2_PENALTY=0.801345 PRIMER_RIGHT_2_PENALTY=0.419105 PRIMER_LEFT_2_SEQUENCE=AACACCAGGGGGTGTCTCTT PRIMER_RIGHT_2_SEQUENCE=AAATAAGGCTGATCCCTGCC PRIMER_LEFT_2=88,20 PRIMER_RIGHT_2=258,20 PRIMER_LEFT_2_TM=60.801 PRIMER_RIGHT_2_TM=60.419 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=171 PRIMER_PAIR_3_PENALTY=1.368277 PRIMER_LEFT_3_PENALTY=0.801345 PRIMER_RIGHT_3_PENALTY=0.566932 PRIMER_LEFT_3_SEQUENCE=AACACCAGGGGGTGTCTCTT PRIMER_RIGHT_3_SEQUENCE=ATAAGGCTGATCCCTGCCTC PRIMER_LEFT_3=88,20 PRIMER_RIGHT_3=256,20 PRIMER_LEFT_3_TM=60.801 PRIMER_RIGHT_3_TM=60.567 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=169 PRIMER_PAIR_4_PENALTY=1.423937 PRIMER_LEFT_4_PENALTY=1.363742 PRIMER_RIGHT_4_PENALTY=0.060196 PRIMER_LEFT_4_SEQUENCE=ACACCAGGGGGTGTCTCTTC PRIMER_RIGHT_4_SEQUENCE=AATAAGGCTGATCCCTGCCT PRIMER_LEFT_4=89,20 PRIMER_RIGHT_4=257,20 PRIMER_LEFT_4_TM=61.364 PRIMER_RIGHT_4_TM=60.060 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=169 = SEQUENCE_ID=31.RHAP61FC4.seq SEQUENCE_TEMPLATE=CCTNCCCCNCNNCNNTGGNCCCNCTCAANNNNNNNNNNNNNNNNNNTCTNCANNNAGNNCGNNACCNTCNATCNCNAGNCANGNTCCNTCCCNNNAACNANNNTCNTCTATAGAAAACNCNCTGGNCGCNCTACGATNNGNNCNNACNNTNNNANNNTNGNNNTNNAANGGNCNNNNNNNGCNNCGNNGNNCNNACNNNCNNNTNNANNTNNCTNNNTNNNNTCGNNNNGNNCNNTNGNNNNGNNNCNNGCTNCNTGGCTTNNNANNNNNCCGACNNNNNNACCNTTTNCNNTNTTTNNGCNTGCNNCNCGNNCCTCNGGNNNNNNNGNNNNNNNNNTNNNGNTTNCNNNCGNGNNGGCNNNNTNNNNNGNNNNGNNNGNNNNGNTCGGNNNCCGNTNNGNNNNCCCNNCCACNNNCCGNTCGNNNNNCNCNNNCNNGCNNCNACCNCNCGGNNNNCNNNGNNNCNNNCNNCNCNGNNTGGNGCGNNCGGCNNNCNGNNGNTGNNNNNNNCNNCCNGNNNNNNNGNTCNNNNGTNGGGNNTCCNNNCCNNTNCNNNNNNNNNCNNNCNGNNNCGNNNGGC SEQUENCE_INCLUDED_REGION=0,580 SEQUENCE_TARGET=29,17 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=33.RHAP61FC12.seq SEQUENCE_TEMPLATE=TCCACATNNCAAGTTCCAGGTCAACCAGGGTTNNGTGGTGAGACCTCCCCCTTCTCTTACGCACACGCCAAACCTTAACAAACCCCCTAAAAGCCAAACCNTGAGAAAACAGAAACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANNTCANAGTCNCTTCNAGANTTGCATACCTAATATGNGCCNTTATCTATGTNNGCAAATGAANGTGAGTCTATANTTTNTNATACCANNGGGGGAGCNTTTGGGCNGCCCAGGGAGCTTTGGTGGGAGAGNATANAGNGGGGNGNNCNNNTCNTGGNAANNAGGAAATCTCNTTNNGNNNNTTNNTGNTANGTCCNGGAGTNTGTGTATNNNATCCTTNNGNTTTNTNNTNNGANATCNNTCCNCTAGGGNTCTTGGTCNNGGGTCNNNGTNNNNNNCGGGTGGAAAAT SEQUENCE_INCLUDED_REGION=0,469 SEQUENCE_TARGET=118,60 P3_COMMENT=CL is GACCTCCCCCTTCTCTTACG P3_COMMENT=CR is CTCCCACCAAAGCTCCCT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.257636 PRIMER_LEFT_0_PENALTY=0.066676 PRIMER_RIGHT_0_PENALTY=2.190960 PRIMER_LEFT_0_SEQUENCE=GACCTCCCCCTTCTCTTACG PRIMER_RIGHT_0_SEQUENCE=CTCCCACCAAAGCTCCCT PRIMER_LEFT_0=41,20 PRIMER_RIGHT_0=307,18 PRIMER_LEFT_0_TM=60.067 PRIMER_RIGHT_0_TM=60.191 PRIMER_LEFT_0_GC_PERCENT=60.000 PRIMER_RIGHT_0_GC_PERCENT=61.111 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.7000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=267 PRIMER_PAIR_1_PENALTY=2.376903 PRIMER_LEFT_1_PENALTY=0.185942 PRIMER_RIGHT_1_PENALTY=2.190960 PRIMER_LEFT_1_SEQUENCE=TGAGACCTCCCCCTTCTCTT PRIMER_RIGHT_1_SEQUENCE=CTCCCACCAAAGCTCCCT PRIMER_LEFT_1=38,20 PRIMER_RIGHT_1=307,18 PRIMER_LEFT_1_TM=60.186 PRIMER_RIGHT_1_TM=60.191 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=270 PRIMER_PAIR_2_PENALTY=2.529770 PRIMER_LEFT_2_PENALTY=0.338810 PRIMER_RIGHT_2_PENALTY=2.190960 PRIMER_LEFT_2_SEQUENCE=GTGAGACCTCCCCCTTCTCT PRIMER_RIGHT_2_SEQUENCE=CTCCCACCAAAGCTCCCT PRIMER_LEFT_2=37,20 PRIMER_RIGHT_2=307,18 PRIMER_LEFT_2_TM=59.661 PRIMER_RIGHT_2_TM=60.191 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=271 PRIMER_PAIR_3_PENALTY=2.564318 PRIMER_LEFT_3_PENALTY=0.373358 PRIMER_RIGHT_3_PENALTY=2.190960 PRIMER_LEFT_3_SEQUENCE=CACACGCCAAACCTTAACAA PRIMER_RIGHT_3_SEQUENCE=CTCCCACCAAAGCTCCCT PRIMER_LEFT_3=61,20 PRIMER_RIGHT_3=307,18 PRIMER_LEFT_3_TM=59.627 PRIMER_RIGHT_3_TM=60.191 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=61.111 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=247 PRIMER_PAIR_4_PENALTY=2.625703 PRIMER_LEFT_4_PENALTY=0.434743 PRIMER_RIGHT_4_PENALTY=2.190960 PRIMER_LEFT_4_SEQUENCE=CCAAACCTTAACAAACCCCC PRIMER_RIGHT_4_SEQUENCE=CTCCCACCAAAGCTCCCT PRIMER_LEFT_4=67,20 PRIMER_RIGHT_4=307,18 PRIMER_LEFT_4_TM=60.435 PRIMER_RIGHT_4_TM=60.191 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=61.111 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=12.4000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=241 = SEQUENCE_ID=34.RHAP61FD10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCTGGAGGATTTCTTTTTCTCTGATAGTATTTAATGTTTCTTTTTCTCTTCTTCAATTGAGGTTCCCTTTTCCTGTGTAAGTCAGAGTTCTTATTTCCACTTCAGTCTCTCAGACAGTTTCGATTACANNNNNNNNNNNNNNNNCCACTTAGATTTCTATTCAGGACAGAAGTCAAATTACTTTACTCGGCTGTTAATGCTTTTCAATTGCACAGAAAAAGGCTCTAGATCTTTGGTACCGAGTTTGCTAACCAATGTGACAGACCCTTTGTTTTAAAATTATGAACGGGGGAAAACAAATAGAGGAAATAAATTCNNAATAGCATGAATTTATACTTCCNGAAATGACCAAAGGGAAAGCCAGGTCCCATNATTACCTTGNGATGCAGAGCAGCNGGNTCAGNACAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,411 SEQUENCE_TARGET=146,16 P3_COMMENT=CL is TGAGGTTCCCTTTTCCTGTG P3_COMMENT=CR is TGAAAAGCATTAACAGCCGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.637239 PRIMER_LEFT_0_PENALTY=0.080729 PRIMER_RIGHT_0_PENALTY=0.556510 PRIMER_LEFT_0_SEQUENCE=TGAGGTTCCCTTTTCCTGTG PRIMER_RIGHT_0_SEQUENCE=TGAAAAGCATTAACAGCCGA PRIMER_LEFT_0=75,20 PRIMER_RIGHT_0=223,20 PRIMER_LEFT_0_TM=60.081 PRIMER_RIGHT_0_TM=59.443 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=11.4000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=149 PRIMER_PAIR_1_PENALTY=1.123197 PRIMER_LEFT_1_PENALTY=0.080729 PRIMER_RIGHT_1_PENALTY=1.042469 PRIMER_LEFT_1_SEQUENCE=TGAGGTTCCCTTTTCCTGTG PRIMER_RIGHT_1_SEQUENCE=TTGAAAAGCATTAACAGCCG PRIMER_LEFT_1=75,20 PRIMER_RIGHT_1=224,20 PRIMER_LEFT_1_TM=60.081 PRIMER_RIGHT_1_TM=58.958 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=11.4000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=150 PRIMER_PAIR_2_PENALTY=1.186120 PRIMER_LEFT_2_PENALTY=0.080729 PRIMER_RIGHT_2_PENALTY=1.105391 PRIMER_LEFT_2_SEQUENCE=TGAGGTTCCCTTTTCCTGTG PRIMER_RIGHT_2_SEQUENCE=GCCTTTTTCTGTGCAATTGA PRIMER_LEFT_2=75,20 PRIMER_RIGHT_2=240,20 PRIMER_LEFT_2_TM=60.081 PRIMER_RIGHT_2_TM=58.895 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=166 PRIMER_PAIR_3_PENALTY=1.205774 PRIMER_LEFT_3_PENALTY=0.080729 PRIMER_RIGHT_3_PENALTY=1.125045 PRIMER_LEFT_3_SEQUENCE=TGAGGTTCCCTTTTCCTGTG PRIMER_RIGHT_3_SEQUENCE=AGCCTTTTTCTGTGCAATTGA PRIMER_LEFT_3=75,20 PRIMER_RIGHT_3=241,21 PRIMER_LEFT_3_TM=60.081 PRIMER_RIGHT_3_TM=59.875 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=167 PRIMER_PAIR_4_PENALTY=1.205774 PRIMER_LEFT_4_PENALTY=0.080729 PRIMER_RIGHT_4_PENALTY=1.125045 PRIMER_LEFT_4_SEQUENCE=TGAGGTTCCCTTTTCCTGTG PRIMER_RIGHT_4_SEQUENCE=GAGCCTTTTTCTGTGCAATTG PRIMER_LEFT_4=75,20 PRIMER_RIGHT_4=242,21 PRIMER_LEFT_4_TM=60.081 PRIMER_RIGHT_4_TM=59.875 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=7.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=168 = SEQUENCE_ID=36.RHAP61FD12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCTCAGCCCGTGTCATTTGACGCTTTCTTAGCACAATATTCCAGACTCTTTCACCTTTTGGTTATGATATAGTTCAAGCAGTTAAAAATCACGGAGGTTCATCACAGAAAGGGTGATGCTTGCTTTTTTTGGTACCAAGTTCTANCNTTGTTTAATTTCTTTGACCTTCCTCTACAATCTCATTTATTTTCAGTCAGGCGAGTGTCTGTGAGTGNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGCACTCATGTGCATACACACAGACGTTAGAAAAAGCCAGTTCCCCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,315 SEQUENCE_TARGET=236,48 P3_COMMENT=CL is TTCAGTCAGGCGAGTGTCTG P3_COMMENT=CR is TCTAGGGGAACTGGCTTTTTC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.466551 PRIMER_LEFT_0_PENALTY=0.178378 PRIMER_RIGHT_0_PENALTY=1.288173 PRIMER_LEFT_0_SEQUENCE=TTCAGTCAGGCGAGTGTCTG PRIMER_RIGHT_0_SEQUENCE=TCTAGGGGAACTGGCTTTTTC PRIMER_LEFT_0=207,20 PRIMER_RIGHT_0=334,21 PRIMER_LEFT_0_TM=60.178 PRIMER_RIGHT_0_TM=59.712 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=7.3000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=128 PRIMER_PAIR_1_PENALTY=1.514665 PRIMER_LEFT_1_PENALTY=0.794652 PRIMER_RIGHT_1_PENALTY=0.720013 PRIMER_LEFT_1_SEQUENCE=GGGTGATGCTTGCTTTTTTT PRIMER_RIGHT_1_SEQUENCE=GTGTATGCACATGAGTGCGA PRIMER_LEFT_1=129,20 PRIMER_RIGHT_1=303,20 PRIMER_LEFT_1_TM=59.205 PRIMER_RIGHT_1_TM=59.280 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.6000 PRIMER_RIGHT_1_END_STABILITY=10.2000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=175 PRIMER_PAIR_2_PENALTY=1.684835 PRIMER_LEFT_2_PENALTY=0.396662 PRIMER_RIGHT_2_PENALTY=1.288173 PRIMER_LEFT_2_SEQUENCE=AGTCAGGCGAGTGTCTGTGA PRIMER_RIGHT_2_SEQUENCE=TCTAGGGGAACTGGCTTTTTC PRIMER_LEFT_2=210,20 PRIMER_RIGHT_2=334,21 PRIMER_LEFT_2_TM=59.603 PRIMER_RIGHT_2_TM=59.712 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.3000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=125 PRIMER_PAIR_3_PENALTY=1.684835 PRIMER_LEFT_3_PENALTY=0.396662 PRIMER_RIGHT_3_PENALTY=1.288173 PRIMER_LEFT_3_SEQUENCE=TCAGTCAGGCGAGTGTCTGT PRIMER_RIGHT_3_SEQUENCE=TCTAGGGGAACTGGCTTTTTC PRIMER_LEFT_3=208,20 PRIMER_RIGHT_3=334,21 PRIMER_LEFT_3_TM=59.603 PRIMER_RIGHT_3_TM=59.712 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=127 PRIMER_PAIR_4_PENALTY=1.927370 PRIMER_LEFT_4_PENALTY=0.178378 PRIMER_RIGHT_4_PENALTY=1.748991 PRIMER_LEFT_4_SEQUENCE=TTCAGTCAGGCGAGTGTCTG PRIMER_RIGHT_4_SEQUENCE=AGGGGAACTGGCTTTTTCTAA PRIMER_LEFT_4=207,20 PRIMER_RIGHT_4=331,21 PRIMER_LEFT_4_TM=60.178 PRIMER_RIGHT_4_TM=59.251 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=125 = SEQUENCE_ID=36.RHAP61FD6.seq SEQUENCE_TEMPLATE=GTCTGAGACCCAGCAANATGNGGGACAGAAATAGAAGACCGACCTGTAATTTCATGGACAAGTTAATCTGGAGTTTACAGTACAGCGGAAACAAAAGANNTCCCGCCTCAGCAAAGTGGAAAGGNGAGAACCAANTCCTGAAAATTATCCTCTGATCTCCATTAGTGTTCCATAGCACCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNACATTNNNNNNNNNNNNNNTGCNANTNTCNCTTNCTNNAAAGANCAACNATGGNNGCACTTTCTCTCTTTAGCNGACCCANNGGNGCGGGTNNTGTNAACTCNNGNTTCTGNGGANNGGGGGTANCGGNGCNCGNNTTNGTTGNNCNTGGTCNTNGCNGTTTCCTTGTTGTGANNTTGTGNNTGCAGCGNNCNGTTCNANNNANNNTGGCGNGCCGGGAGNNTTNAGNGGAAANCCTGNNNGTTGCTTTNTGNNGGNGAGCTANGTCNCNTNNTTTGGGTNNNNNNNNNGGGNCNCTTTT SEQUENCE_INCLUDED_REGION=0,530 SEQUENCE_TARGET=182,46 SEQUENCE_TARGET=235,14 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=38.RHAP61FE5.seq SEQUENCE_TEMPLATE=NANACCNCCCCNANCCTGNCCTCTATCTCNNNCCNNNNNNNNNNNNNNNNNNNNTNCNTCCTCTCTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGAATTAATTNTAGGTGTGGTAAGCATTTGGTGCCAGCATTAATTTCCAGTCCCACCTTAAGAGNTTACTAGTGCGAANCTGAANTAANATTGACACAGTGAACGACGANTGCTGGGTAAGNGTCNNAGGGGNNCTTTGTNNTCACTCNCTACACTTAGGTGTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,320 SEQUENCE_TARGET=34,20 SEQUENCE_TARGET=69,82 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=39.RHAP61FE6.seq SEQUENCE_TEMPLATE=NANNCNCCTAGAGGACCCTGTCCNGCCAAACATTGTNTTCATGTCATGGACAAGGACAGGGACAGGGACCNTNAAGTTNCTTTTNNCCTACAGGCNCTGCCAATCCTCTGCTTCTGTCATCCCCATAGCATGTCNTAGNGCTGGACATTACACACCNTTCANCGTNNNCNNCNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNGTNTNNCACAAGCGCCNGCTATCNTGANCCCNNCATAATNNTGGCCGNGTAGTANTCCNNANTGNTTATCTGCTNNCTATGTTTGTANGNNNGANAGNAGGATTNATGNNNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,370 SEQUENCE_TARGET=177,50 SEQUENCE_TARGET=230,25 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=41.RHAP61FF11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNTGTCTGTCAAAACTCCGGTCATCTTCGAGACTTGACACTGGTATTTTTGTGTATGGACCNTTTCCTAGTCACATAGACCTCATTCACAGTGGATANTANTGNGTCTCCCATCAAGTCCCTGAGTCACTGACCTAAGTACAGGCTGTATCATTGACACCATATTTNNTTCTGTTTCTTNNTCGTGGAAGTGNTACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGATGTGNGTGNGNTGTANNGNNNNNATNTNGTANNNNNTNNNNNAGANNNNAAAGGTCAANNNNNAGNNCNNTNTNTGNNAAAAANNNANNNNCNTTANTNTGANANAACTNTCNCNNAGAANGGGNNNCNNNNCNCNAATTCGCTNNNNANGNNAANAGNNNGNNNCNNNNNGNGAAANNNNNNNNCGCNCAAAAAANTNNANGAAAAANANG SEQUENCE_INCLUDED_REGION=20,464 SEQUENCE_TARGET=216,50 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=41.RHAP61FF5.seq SEQUENCE_TEMPLATE=TNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNTGNNATCCCNGGNGGTGACTGTGGGNGCATCNGTNANNGNGGNNGNGTNNNTGTNNCNAANNGTTGTGGGAGTCNNTNNTGTGNCCCTGGTGGNTGNAACTANNTGTAGNTGNTAAGAACACCNGTGGNGAGNTAAGAGNTCTNNCATGGACCGGGNANTNAGNTGGTAANCGNNCGCNAACNCGNNATCANNNNNANANNNNGTTTCCNNGNNNNAAAANNNNNANCCNNNGANNNTNNCNNNANANANTNGNNGNCCGGGGAANANNANGNNNNNANCNTGGGGGGTGNCNNTANGCCGNGAGNNNNNNNNNNANNNNNNTGGGCNNGNNNTCNNNTGNNNNGGNNGTCNANNNNNNNANANGNNTGNNGGNNCNAGNNGNNTNNNGNGAAAGGNNGNNAANNTGCGNGGANANGGNNNTTTGNNTANNGGGAGNATGNT SEQUENCE_INCLUDED_REGION=0,494 SEQUENCE_TARGET=9,26 SEQUENCE_TARGET=37,14 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=42.RHAP61FF12.seq SEQUENCE_TEMPLATE=NNNNNNTGTAATCCTGTAATCCAGGGACGTGCTTTTCTGTGTTAGTTGGAAATCTCCAGTAAGTAAAACCATGTGNGNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTATAACTCCATCCATCCAATATCTAGTATACATNTATATACATTAGAGTTATTTATTACAAAGTACTGATGGACATGATTATNGANGCCAAGAAGTTCCAATTATTTTGAGTGAATTTCNNNAAAAGCCCANACTTANAANAGGAAAATAAAGCCTNTCTCCCTGTNGACAAANTGTCCNTGCGATAANCCTATAANCCCCNAACCCTTTTGGGAACAGTGCCAACCGNNNGGGGGNGGNAAAATTTCATATNNNTGNGGGCCCNNTGGGGNGTCANNTCTGTTNNAAGNNNCAATNNNNAAGGNNANNTTGNNANGGGGTGANCGNGGGNNTGGNNTTCGGNNTTNAANGGGGCNNNAGNTGTNTCCNGGT SEQUENCE_INCLUDED_REGION=6,483 SEQUENCE_TARGET=82,32 P3_COMMENT=CL is CTGTAATCCAGGGACGTGCT P3_COMMENT=CR is ACTAGATATTGGATGGATGGAGTT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.958177 PRIMER_LEFT_0_PENALTY=0.133741 PRIMER_RIGHT_0_PENALTY=6.824436 PRIMER_LEFT_0_SEQUENCE=CTGTAATCCAGGGACGTGCT PRIMER_RIGHT_0_SEQUENCE=ACTAGATATTGGATGGATGGAGTT PRIMER_LEFT_0=13,20 PRIMER_RIGHT_0=143,24 PRIMER_LEFT_0_TM=60.134 PRIMER_RIGHT_0_TM=57.176 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=37.500 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=131 PRIMER_PAIR_1_PENALTY=7.255493 PRIMER_LEFT_1_PENALTY=0.431057 PRIMER_RIGHT_1_PENALTY=6.824436 PRIMER_LEFT_1_SEQUENCE=TAATCCAGGGACGTGCTTTT PRIMER_RIGHT_1_SEQUENCE=ACTAGATATTGGATGGATGGAGTT PRIMER_LEFT_1=16,20 PRIMER_RIGHT_1=143,24 PRIMER_LEFT_1_TM=59.569 PRIMER_RIGHT_1_TM=57.176 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=37.500 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=128 PRIMER_PAIR_2_PENALTY=7.341119 PRIMER_LEFT_2_PENALTY=0.516683 PRIMER_RIGHT_2_PENALTY=6.824436 PRIMER_LEFT_2_SEQUENCE=TGTAATCCAGGGACGTGCTT PRIMER_RIGHT_2_SEQUENCE=ACTAGATATTGGATGGATGGAGTT PRIMER_LEFT_2=14,20 PRIMER_RIGHT_2=143,24 PRIMER_LEFT_2_TM=60.517 PRIMER_RIGHT_2_TM=57.176 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=37.500 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=130 PRIMER_PAIR_3_PENALTY=7.568314 PRIMER_LEFT_3_PENALTY=0.133741 PRIMER_RIGHT_3_PENALTY=7.434573 PRIMER_LEFT_3_SEQUENCE=CTGTAATCCAGGGACGTGCT PRIMER_RIGHT_3_SEQUENCE=GATATTGGATGGATGGAGTTATAGA PRIMER_LEFT_3=13,20 PRIMER_RIGHT_3=139,25 PRIMER_LEFT_3_TM=60.134 PRIMER_RIGHT_3_TM=57.565 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=36.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=5.6000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=127 PRIMER_PAIR_4_PENALTY=7.737137 PRIMER_LEFT_4_PENALTY=0.912701 PRIMER_RIGHT_4_PENALTY=6.824436 PRIMER_LEFT_4_SEQUENCE=TCCTGTAATCCAGGGACGTG PRIMER_RIGHT_4_SEQUENCE=ACTAGATATTGGATGGATGGAGTT PRIMER_LEFT_4=11,20 PRIMER_RIGHT_4=143,24 PRIMER_LEFT_4_TM=60.913 PRIMER_RIGHT_4_TM=57.176 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=37.500 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=133 = SEQUENCE_ID=44.RHAP61FG5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNTATCTCTGACTTTGTTGTTTTGNTTTTCCGCANTTTTCATTGTGAATTCNATTTTTGNTTGNCTTTNATTTGCTGACNATCTCTTTATTAAANTTNANGTCTTGAATTGNTTCANTTATTTCACCCAACNANTCTNNNGGGGTANTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGCNTATGGGGGTGNNTTTTACAGTCTCCCNNNAGANATNNATGGTCTATNNTCNNTAAGANCNNTGGACATANTCATAANNACNANTCAGTTAACANNGCNCCNNNAAATTAGNNNNTCNATNNANAATTGNNAGTGGNNAGTCCNTGGCCNGGGCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=10,395 SEQUENCE_TARGET=159,84 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=47.RHAP61FH11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTGTCAAAATTAAAAACCAGCAAGAAAGGTCACTTNNATTTTAAGTGGAGAGAGGAAATAATTNANGGATAAAATATAAAACAATAATGAAAAATTAATTTCATCATGGACTGCNTATTAAGANNNANNTGTACATAAGNGNTATTGTTGTGTCANNAGTTAACTTCCCNGNTANCCCGNACAGGNNNTNGGGTCTANNANNCNNNANGGCCNACNTNAANNTTAAATGNCANNTTGTTTTCNGGNGTGGNTTCAATGCNCTNTGNNNTNNNNCNCNNNGNNNNNNNNNNNNNNNNNNNNTGNTGNNNNNNNNNNNNNNNNNGNNNANAANNGGTNTNAANNANTTANGNTNNNNNGNNATNNGNGNNGNTNGTGTGNNNNNNGGTNNTGNTNGNGTGNTNCNNTGTTGNTNNNNNNNNNNTNNNNGANNGNATNNTGNCNAANNNNNNCGNNNNNNNNNNCTTNTTGNANNGNTNNGNATTGNNNNTTT SEQUENCE_INCLUDED_REGION=19,489 SEQUENCE_TARGET=300,18 SEQUENCE_TARGET=323,16 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=48.RHAP61FH6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNTTGTGTCANACTNACAAAAATCNAACCAGGACACAATGTTNANCTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAGTTTATGTCCTTTTCATCTCTTGTAGCATTTAGTATGCACTGAGGCCATATCTTTTGAAGTGAGAGTTATACTGACATATTAGTATAAATTTCTTTTTGCATCTCTGNAGTTTTATAGGATTGTGTGTANTGTAGAATCTAACAAACTTTANTAAGTCTAGCATCTCACCTAAAGGAAAGNNCTCTGGGGGCCTACACTGGGGTGTGACCAAACTGCCTGGCACCAGTAGGCGCTCTCCATGGGGGGNCATTCTTCAGGTACAATAAGGGGTTGTCTGTTCAATACAGCNGTGGGGGGAACNTGCTGGCTGATAAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=10,433 SEQUENCE_TARGET=58,62 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=01.RHAP64FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNCAACGGGNTGGCTCAGTGGNTAGAGGTACTTGCCCACCNAGTTTTAACAACTGGAGTTCGGATCCCAGGGTCCCACGTCATGGAAGACAGGGAAAGNNTCCCACANTGGTTTACACACACTNCNTCGCACACAGTCACGCGCANGCGNAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGAGCACCAGAGGACGACAGAGGACAACAGAGAGCTNNNCNGTCACAGNCTCCNGACNNNNNNGTAATTAAGANNTTTGAGNAGNNNCNNNNGTGTTNGNTGNCAAAGAANAGNGNGGNNNTTNNNNNGNNAGTGGTGNANAGGNANNTTGGNCNGGNANGGAGGGCNNNNCCNTGGNGGNNGGNTNNNAAGNNNNNTGCNGNNNGANNGGNGNNNCGTGGNTGGGNNNGNANACNAGNNNNNNNANNNNNNTTGNNNNNGNNNTNNNNANCCNGGNGTNNNGAGNANNNGTTNNNNNNGAGNGNNNGTNNNC SEQUENCE_INCLUDED_REGION=25,495 SEQUENCE_TARGET=176,28 P3_COMMENT=CL is ACGTCATGGAAGACAGGGAA P3_COMMENT=CR is CTCTGTCGTCCTCTGGTGCT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.118415 PRIMER_LEFT_0_PENALTY=0.510331 PRIMER_RIGHT_0_PENALTY=0.608084 PRIMER_LEFT_0_SEQUENCE=ACGTCATGGAAGACAGGGAA PRIMER_RIGHT_0_SEQUENCE=CTCTGTCGTCCTCTGGTGCT PRIMER_LEFT_0=99,20 PRIMER_RIGHT_0=228,20 PRIMER_LEFT_0_TM=60.510 PRIMER_RIGHT_0_TM=60.608 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=130 PRIMER_PAIR_1_PENALTY=1.236221 PRIMER_LEFT_1_PENALTY=0.510331 PRIMER_RIGHT_1_PENALTY=0.725890 PRIMER_LEFT_1_SEQUENCE=ACGTCATGGAAGACAGGGAA PRIMER_RIGHT_1_SEQUENCE=GTCCTCTGTCGTCCTCTGGT PRIMER_LEFT_1=99,20 PRIMER_RIGHT_1=231,20 PRIMER_LEFT_1_TM=60.510 PRIMER_RIGHT_1_TM=59.274 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=8.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=133 PRIMER_PAIR_2_PENALTY=1.546072 PRIMER_LEFT_2_PENALTY=0.510331 PRIMER_RIGHT_2_PENALTY=1.035741 PRIMER_LEFT_2_SEQUENCE=ACGTCATGGAAGACAGGGAA PRIMER_RIGHT_2_SEQUENCE=TTGTCCTCTGTCGTCCTCTG PRIMER_LEFT_2=99,20 PRIMER_RIGHT_2=233,20 PRIMER_LEFT_2_TM=60.510 PRIMER_RIGHT_2_TM=58.964 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=8.00 PRIMER_PAIR_2_COMPL_END=6.00 PRIMER_PAIR_2_PRODUCT_SIZE=135 PRIMER_PAIR_3_PENALTY=1.615970 PRIMER_LEFT_3_PENALTY=1.007886 PRIMER_RIGHT_3_PENALTY=0.608084 PRIMER_LEFT_3_SEQUENCE=AACAACTGGAGTTCGGATCC PRIMER_RIGHT_3_SEQUENCE=CTCTGTCGTCCTCTGGTGCT PRIMER_LEFT_3=70,20 PRIMER_RIGHT_3=228,20 PRIMER_LEFT_3_TM=58.992 PRIMER_RIGHT_3_TM=60.608 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.8000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=159 PRIMER_PAIR_4_PENALTY=1.618571 PRIMER_LEFT_4_PENALTY=0.510331 PRIMER_RIGHT_4_PENALTY=1.108240 PRIMER_LEFT_4_SEQUENCE=ACGTCATGGAAGACAGGGAA PRIMER_RIGHT_4_SEQUENCE=GTTGTCCTCTGTCGTCCTCTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=234,21 PRIMER_LEFT_4_TM=60.510 PRIMER_RIGHT_4_TM=59.892 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=57.143 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=8.00 PRIMER_PAIR_4_COMPL_END=6.00 PRIMER_PAIR_4_PRODUCT_SIZE=136 = SEQUENCE_ID=01.RHAP64FA7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCACANAAAACATCCTGTCNNNNNNNNNNNNNNNNTAGCTNNNNNNNNNNNNNNNNTATAAGTGCATGGAATGAGTGTGTATANNNNNNNNNNNNNNNNNNNNNNNNTTAGCTTANNNNNNNNNNNNNNNNNNNNNNNNTATAAGTGCATGGAAGGAGTGTGTGTATGTGACTGTGTGCTGAATATGTAAGCGTGNGGCGGTAAANNNNNNNNNNNNNNNNNNNNNNTAANTGNNNGACNNAAGCCNCAANGNGCGCTNTGTGCACTGTGTCNATNCGCCCGNGTCANCACNGCCACNCANNCANAACANGCCNNNAGCGNCGTGCATGCTGANCCGNCCCATATCNGNGNNNACCTANNNNACAACNNCANNNNCCGAAAGCNNNCGCCATCCCCNTANACANGNCCCCNNCNGCCACTNCNNCANCNNNGANNCACNTCTGANCCNNGCNCATNTCNNANNNANGACCNCTNNNTCCCGNC SEQUENCE_INCLUDED_REGION=22,483 SEQUENCE_TARGET=43,14 SEQUENCE_TARGET=62,16 SEQUENCE_TARGET=105,24 SEQUENCE_TARGET=137,24 SEQUENCE_TARGET=227,22 P3_COMMENT=CL is AAGTGCATGGAATGAGTGTGT P3_COMMENT=CR is CGCTTACATATTCAGCACACAG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.387345 PRIMER_LEFT_0_PENALTY=2.927648 PRIMER_RIGHT_0_PENALTY=3.459697 PRIMER_LEFT_0_SEQUENCE=AAGTGCATGGAATGAGTGTGT PRIMER_RIGHT_0_SEQUENCE=CGCTTACATATTCAGCACACAG PRIMER_LEFT_0=81,21 PRIMER_RIGHT_0=214,22 PRIMER_LEFT_0_TM=58.072 PRIMER_RIGHT_0_TM=58.540 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=134 PRIMER_PAIR_1_PENALTY=6.393094 PRIMER_LEFT_1_PENALTY=2.933397 PRIMER_RIGHT_1_PENALTY=3.459697 PRIMER_LEFT_1_SEQUENCE=AAGTGCATGGAATGAGTGTG PRIMER_RIGHT_1_SEQUENCE=CGCTTACATATTCAGCACACAG PRIMER_LEFT_1=81,20 PRIMER_RIGHT_1=214,22 PRIMER_LEFT_1_TM=57.067 PRIMER_RIGHT_1_TM=58.540 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=134 PRIMER_PAIR_2_PENALTY=6.439443 PRIMER_LEFT_2_PENALTY=2.927648 PRIMER_RIGHT_2_PENALTY=3.511795 PRIMER_LEFT_2_SEQUENCE=AAGTGCATGGAATGAGTGTGT PRIMER_RIGHT_2_SEQUENCE=CGCTTACATATTCAGCACACA PRIMER_LEFT_2=81,21 PRIMER_RIGHT_2=214,21 PRIMER_LEFT_2_TM=58.072 PRIMER_RIGHT_2_TM=57.488 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=5.00 PRIMER_PAIR_2_PRODUCT_SIZE=134 PRIMER_PAIR_3_PENALTY=6.439443 PRIMER_LEFT_3_PENALTY=2.927648 PRIMER_RIGHT_3_PENALTY=3.511795 PRIMER_LEFT_3_SEQUENCE=AAGTGCATGGAATGAGTGTGT PRIMER_RIGHT_3_SEQUENCE=CACGCTTACATATTCAGCACA PRIMER_LEFT_3=81,21 PRIMER_RIGHT_3=216,21 PRIMER_LEFT_3_TM=58.072 PRIMER_RIGHT_3_TM=57.488 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=136 PRIMER_PAIR_4_PENALTY=6.445192 PRIMER_LEFT_4_PENALTY=2.933397 PRIMER_RIGHT_4_PENALTY=3.511795 PRIMER_LEFT_4_SEQUENCE=AAGTGCATGGAATGAGTGTG PRIMER_RIGHT_4_SEQUENCE=CGCTTACATATTCAGCACACA PRIMER_LEFT_4=81,20 PRIMER_RIGHT_4=214,21 PRIMER_LEFT_4_TM=57.067 PRIMER_RIGHT_4_TM=57.488 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=134 = SEQUENCE_ID=02.RHAP64FA8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCANTGACANAGCCTCTCTTGAAAATGAGGTTGAGGACCTGCCTCAGGAACAGGAGCCAGGGTCANTTGTAGCCTCTACACTCTTTTGCANTGAGGTGCAGGTCATATAGATGCAAGGGGCAAAAGGGGATCGGCACGTGCGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGACAAANACCCAGATTCATANAAAANACAGTNANGTAGGAACAGGNNCACACATACTTANACANNNGTAAACGTGGAAAAATGGCANATNNNNNNNNNNNNNNNNNNNNNNNNNNCCAGGGNNNNACACACCACACAAAAGANGTGGTNNNNACATNCNAACATNNACNNNAACNANNGTGNNAANTCACNNANTNNTNCNTNTNNTGGNAANNANNCANGNAAAAGTGTNNACACACTTANANNNAGGCAAATNTGTNNGNA SEQUENCE_INCLUDED_REGION=12,459 SEQUENCE_TARGET=156,50 SEQUENCE_TARGET=297,26 P3_COMMENT=CL is TAGATGCAAGGGGCAAAAG P3_COMMENT=CR is TGCCATTTTTCCACGTTTAC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.612869 PRIMER_LEFT_0_PENALTY=2.144984 PRIMER_RIGHT_0_PENALTY=1.467885 PRIMER_LEFT_0_SEQUENCE=TAGATGCAAGGGGCAAAAG PRIMER_RIGHT_0_SEQUENCE=TGCCATTTTTCCACGTTTAC PRIMER_LEFT_0=119,19 PRIMER_RIGHT_0=293,20 PRIMER_LEFT_0_TM=58.855 PRIMER_RIGHT_0_TM=58.532 PRIMER_LEFT_0_GC_PERCENT=47.368 PRIMER_RIGHT_0_GC_PERCENT=40.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=6.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=175 PRIMER_PAIR_1_PENALTY=3.802447 PRIMER_LEFT_1_PENALTY=2.334562 PRIMER_RIGHT_1_PENALTY=1.467885 PRIMER_LEFT_1_SEQUENCE=TAGATGCAAGGGGCAAAAGG PRIMER_RIGHT_1_SEQUENCE=TGCCATTTTTCCACGTTTAC PRIMER_LEFT_1=119,20 PRIMER_RIGHT_1=293,20 PRIMER_LEFT_1_TM=62.335 PRIMER_RIGHT_1_TM=58.532 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=6.0000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=175 PRIMER_PAIR_2_PENALTY=4.632377 PRIMER_LEFT_2_PENALTY=3.164492 PRIMER_RIGHT_2_PENALTY=1.467885 PRIMER_LEFT_2_SEQUENCE=AAGGGGCAAAAGGGGATC PRIMER_RIGHT_2_SEQUENCE=TGCCATTTTTCCACGTTTAC PRIMER_LEFT_2=126,18 PRIMER_RIGHT_2=293,20 PRIMER_LEFT_2_TM=61.164 PRIMER_RIGHT_2_TM=58.532 PRIMER_LEFT_2_GC_PERCENT=55.556 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.8000 PRIMER_RIGHT_2_END_STABILITY=6.0000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=168 PRIMER_PAIR_3_PENALTY=4.671048 PRIMER_LEFT_3_PENALTY=3.203163 PRIMER_RIGHT_3_PENALTY=1.467885 PRIMER_LEFT_3_SEQUENCE=AGATGCAAGGGGCAAAAG PRIMER_RIGHT_3_SEQUENCE=TGCCATTTTTCCACGTTTAC PRIMER_LEFT_3=120,18 PRIMER_RIGHT_3=293,20 PRIMER_LEFT_3_TM=58.797 PRIMER_RIGHT_3_TM=58.532 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=6.0000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=174 PRIMER_PAIR_4_PENALTY=4.881041 PRIMER_LEFT_4_PENALTY=3.413156 PRIMER_RIGHT_4_PENALTY=1.467885 PRIMER_LEFT_4_SEQUENCE=AGATGCAAGGGGCAAAAGG PRIMER_RIGHT_4_SEQUENCE=TGCCATTTTTCCACGTTTAC PRIMER_LEFT_4=120,19 PRIMER_RIGHT_4=293,20 PRIMER_LEFT_4_TM=62.413 PRIMER_RIGHT_4_TM=58.532 PRIMER_LEFT_4_GC_PERCENT=52.632 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=174 = SEQUENCE_ID=03.RHAP64FA3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCCTGGAACTGACAAAGTAACCCCAATGTTTGTTGGTAACGTCTTTAGTTCCATGAAACTATCACAGGCTTCAGAGTGCTGTTCTGCCCTGTTCACACTACAAATTCAATTTGATTCTCTTCCGTCTCTAAAGTTCTAATGTAAACTGTACAGCTGACAAAGCATAAATAAGGCTTTAAATGCTAAGGGTTCGCTGTAACAGGATGTAACTGTAATTTACTCTCAAATATATCTTTTAAATCTCCACGTGTGTCAGAAAGAGACAAAGACATGCATGCTTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAAGAGGGAGAGGGNNAGGGNGNAGGAAATTGNNAAAATGAAA SEQUENCE_INCLUDED_REGION=21,423 SEQUENCE_TARGET=304,34 SEQUENCE_TARGET=346,54 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=04.RHAP64FB7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCTCCACTGCAATCAAAAGATGANCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTCTCTTATAAGACTTAGGATATTAAAACAAATGAGAGAAATGGACTCTAGGGTATCCAATAGTAAGTCAACAAGGGTTGGTGAGTTCCTGTCCTGTAACAGACCCAAGAGCCACCCACGTCTAATCTGNAGGGANACAAACAGGGGGGCAATAAGGGAATGGGAGAGAGGAAGANAGTCTCATGTCACGCAGAAACGNTCTCCCCGAGCATCNCAAGTGGAGACCTGGGAACAGATTCAGGTAGATTGTAGGAATACAGGGGANATGCCCTAGGNAGCAGCAGGTAGCTGGAGAGAATCNAAGGCTTCTGGGAGCTGATGTGAGAGCATGCACNGAGAAGNNTCCTGCATCTGTCTGTGTCTNAGTTAGTGGTTTCACNNC SEQUENCE_INCLUDED_REGION=12,455 SEQUENCE_TARGET=39,44 P3_COMMENT=CL is CCTCCACTGCAATCAAAAGA P3_COMMENT=CR is CAGGAACTCACCAACCCTTG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.688139 PRIMER_LEFT_0_PENALTY=1.152564 PRIMER_RIGHT_0_PENALTY=0.535575 PRIMER_LEFT_0_SEQUENCE=CCTCCACTGCAATCAAAAGA PRIMER_RIGHT_0_SEQUENCE=CAGGAACTCACCAACCCTTG PRIMER_LEFT_0=12,20 PRIMER_RIGHT_0=175,20 PRIMER_LEFT_0_TM=58.847 PRIMER_RIGHT_0_TM=60.536 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=164 PRIMER_PAIR_1_PENALTY=2.152262 PRIMER_LEFT_1_PENALTY=1.152564 PRIMER_RIGHT_1_PENALTY=0.999698 PRIMER_LEFT_1_SEQUENCE=CCTCCACTGCAATCAAAAGA PRIMER_RIGHT_1_SEQUENCE=GACAGGAACTCACCAACCCT PRIMER_LEFT_1=12,20 PRIMER_RIGHT_1=177,20 PRIMER_LEFT_1_TM=58.847 PRIMER_RIGHT_1_TM=59.000 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=9.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=166 PRIMER_PAIR_2_PENALTY=2.152262 PRIMER_LEFT_2_PENALTY=1.152564 PRIMER_RIGHT_2_PENALTY=0.999698 PRIMER_LEFT_2_SEQUENCE=CCTCCACTGCAATCAAAAGA PRIMER_RIGHT_2_SEQUENCE=AGGACAGGAACTCACCAACC PRIMER_LEFT_2=12,20 PRIMER_RIGHT_2=179,20 PRIMER_LEFT_2_TM=58.847 PRIMER_RIGHT_2_TM=59.000 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=168 PRIMER_PAIR_3_PENALTY=2.261076 PRIMER_LEFT_3_PENALTY=1.152564 PRIMER_RIGHT_3_PENALTY=1.108512 PRIMER_LEFT_3_SEQUENCE=CCTCCACTGCAATCAAAAGA PRIMER_RIGHT_3_SEQUENCE=GGAACTCACCAACCCTTGTT PRIMER_LEFT_3=12,20 PRIMER_RIGHT_3=173,20 PRIMER_LEFT_3_TM=58.847 PRIMER_RIGHT_3_TM=58.891 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=162 PRIMER_PAIR_4_PENALTY=2.266317 PRIMER_LEFT_4_PENALTY=1.152564 PRIMER_RIGHT_4_PENALTY=1.113753 PRIMER_LEFT_4_SEQUENCE=CCTCCACTGCAATCAAAAGA PRIMER_RIGHT_4_SEQUENCE=AGGAACTCACCAACCCTTGTT PRIMER_LEFT_4=12,20 PRIMER_RIGHT_4=174,21 PRIMER_LEFT_4_TM=58.847 PRIMER_RIGHT_4_TM=59.886 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=163 = SEQUENCE_ID=06.RHAP64FB3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCCTTTAAATGATCAAGGAGTATCAAAANTTCCTCATTTGTGAATANANCTACGTGTATGNGTGTGNNAANGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTATCCGTACATGGATCAGAGAAATATAATCCTAAAAATCACAAAAATGACATAAAATGACATCTGTTATTTAAAAAAATCACCTCAGTTCAATGAGAACTAAAATATTTAAATCAACTACAGGAAAAAAAAACACCCCAGCGATTACACACACTGATTCAAAGATTGTGGCACCATTGACATATATTTCATTTTTGNTATAAAGTTTAANGTTTTGTAGGGNTGACTTGAACCCTGAAGAAGGTACCCAGGGTGCAGGTCAGTGCTGCATATGTTATTTACCAGTGCTAGACACTCAAACTTCAGGGTNNNCAC SEQUENCE_INCLUDED_REGION=21,421 SEQUENCE_TARGET=97,28 P3_COMMENT=CL is CCTTTAAATGATCAAGGAGTATCAA P3_COMMENT=CR is TTTCTCTGATCCATGTACGGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=9.945457 PRIMER_LEFT_0_PENALTY=7.104630 PRIMER_RIGHT_0_PENALTY=2.840827 PRIMER_LEFT_0_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA PRIMER_RIGHT_0_SEQUENCE=TTTCTCTGATCCATGTACGGA PRIMER_LEFT_0=21,25 PRIMER_RIGHT_0=150,21 PRIMER_LEFT_0_TM=57.895 PRIMER_RIGHT_0_TM=58.159 PRIMER_LEFT_0_GC_PERCENT=32.000 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=9.6000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=130 PRIMER_PAIR_1_PENALTY=10.575087 PRIMER_LEFT_1_PENALTY=7.104630 PRIMER_RIGHT_1_PENALTY=3.470456 PRIMER_LEFT_1_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA PRIMER_RIGHT_1_SEQUENCE=TTTCTCTGATCCATGTACGGAT PRIMER_LEFT_1=21,25 PRIMER_RIGHT_1=150,22 PRIMER_LEFT_1_TM=57.895 PRIMER_RIGHT_1_TM=58.530 PRIMER_LEFT_1_GC_PERCENT=32.000 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=9.8000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=130 PRIMER_PAIR_2_PENALTY=10.575087 PRIMER_LEFT_2_PENALTY=7.104630 PRIMER_RIGHT_2_PENALTY=3.470456 PRIMER_LEFT_2_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA PRIMER_RIGHT_2_SEQUENCE=ATTTCTCTGATCCATGTACGGA PRIMER_LEFT_2=21,25 PRIMER_RIGHT_2=151,22 PRIMER_LEFT_2_TM=57.895 PRIMER_RIGHT_2_TM=58.530 PRIMER_LEFT_2_GC_PERCENT=32.000 PRIMER_RIGHT_2_GC_PERCENT=40.909 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=9.6000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=131 PRIMER_PAIR_3_PENALTY=11.044168 PRIMER_LEFT_3_PENALTY=7.104630 PRIMER_RIGHT_3_PENALTY=3.939538 PRIMER_LEFT_3_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA PRIMER_RIGHT_3_SEQUENCE=TTCTCTGATCCATGTACGGAT PRIMER_LEFT_3=21,25 PRIMER_RIGHT_3=149,21 PRIMER_LEFT_3_TM=57.895 PRIMER_RIGHT_3_TM=57.060 PRIMER_LEFT_3_GC_PERCENT=32.000 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=9.8000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=129 PRIMER_PAIR_4_PENALTY=11.242315 PRIMER_LEFT_4_PENALTY=7.104630 PRIMER_RIGHT_4_PENALTY=4.137685 PRIMER_LEFT_4_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA PRIMER_RIGHT_4_SEQUENCE=ATTTCTCTGATCCATGTACGGAT PRIMER_LEFT_4=21,25 PRIMER_RIGHT_4=151,23 PRIMER_LEFT_4_TM=57.895 PRIMER_RIGHT_4_TM=58.862 PRIMER_LEFT_4_GC_PERCENT=32.000 PRIMER_RIGHT_4_GC_PERCENT=39.130 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=9.8000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=131 = SEQUENCE_ID=07.RHAP64FC7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCAGCCAAANATCGTCAACAAACGCCTGTGACNACCCCCCAAGATCAAAATCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGTGTCAGTGTCTCTCCCNGCACCCATGGACATATGCATGTGCCGACCCAATANGCGTCCATNTATGCATATCCATGTGCATCTGNNCATGTATGTGCACATGTGTGTCTCTCAGTGTGGGTGNACAGGNGTANGGGACATGTGTGCACATGGGCGCANTCCAAATAATCNGGAACTCTTCCCCAGCTTCACCTTGAAGGCATTCCCAGGCGCAAGCAANAGCTANCGTAACCACNGGCAGCATTCTCTNCTCNCCTCCGACCCCTNTAGCTCTCGCCNCATCAGGCCGTAGGCTGNCCCAGCTTCAAACTGNTAAGAGCNTGTCACCCTCCANNCAGAGGNACCCGTGNGNGTGNGGGTCNGC SEQUENCE_INCLUDED_REGION=12,448 SEQUENCE_TARGET=67,29 P3_COMMENT=CL is CCCCCAAGATCAAAATCCAC P3_COMMENT=CR is ACCCACACTGAGAGACACACA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.260169 PRIMER_LEFT_0_PENALTY=1.441475 PRIMER_RIGHT_0_PENALTY=1.818694 PRIMER_LEFT_0_SEQUENCE=CCCCCAAGATCAAAATCCAC PRIMER_RIGHT_0_SEQUENCE=ACCCACACTGAGAGACACACA PRIMER_LEFT_0=47,20 PRIMER_RIGHT_0=217,21 PRIMER_LEFT_0_TM=61.441 PRIMER_RIGHT_0_TM=59.181 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=171 PRIMER_PAIR_1_PENALTY=3.260169 PRIMER_LEFT_1_PENALTY=1.441475 PRIMER_RIGHT_1_PENALTY=1.818694 PRIMER_LEFT_1_SEQUENCE=CCCCCAAGATCAAAATCCAC PRIMER_RIGHT_1_SEQUENCE=CACCCACACTGAGAGACACAC PRIMER_LEFT_1=47,20 PRIMER_RIGHT_1=218,21 PRIMER_LEFT_1_TM=61.441 PRIMER_RIGHT_1_TM=59.181 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=57.143 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=172 PRIMER_PAIR_2_PENALTY=3.287638 PRIMER_LEFT_2_PENALTY=1.441475 PRIMER_RIGHT_2_PENALTY=1.846163 PRIMER_LEFT_2_SEQUENCE=CCCCCAAGATCAAAATCCAC PRIMER_RIGHT_2_SEQUENCE=CCCACACTGAGAGACACACA PRIMER_LEFT_2=47,20 PRIMER_RIGHT_2=216,20 PRIMER_LEFT_2_TM=61.441 PRIMER_RIGHT_2_TM=58.154 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=170 PRIMER_PAIR_3_PENALTY=3.287638 PRIMER_LEFT_3_PENALTY=1.441475 PRIMER_RIGHT_3_PENALTY=1.846163 PRIMER_LEFT_3_SEQUENCE=CCCCCAAGATCAAAATCCAC PRIMER_RIGHT_3_SEQUENCE=CACCCACACTGAGAGACACA PRIMER_LEFT_3=47,20 PRIMER_RIGHT_3=218,20 PRIMER_LEFT_3_TM=61.441 PRIMER_RIGHT_3_TM=58.154 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=172 PRIMER_PAIR_4_PENALTY=3.462692 PRIMER_LEFT_4_PENALTY=1.643998 PRIMER_RIGHT_4_PENALTY=1.818694 PRIMER_LEFT_4_SEQUENCE=CCCCCAAGATCAAAATCCA PRIMER_RIGHT_4_SEQUENCE=ACCCACACTGAGAGACACACA PRIMER_LEFT_4=47,19 PRIMER_RIGHT_4=217,21 PRIMER_LEFT_4_TM=60.644 PRIMER_RIGHT_4_TM=59.181 PRIMER_LEFT_4_GC_PERCENT=47.368 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=171 = SEQUENCE_ID=09.RHAP64FC3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCTGACATTCTCTTCCGACCTCTGCAGCCATCAGCATATGTGGTGCACAGACATATGTGTAGGCAAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACAATTGTTTTTAAGGAAAAAAGTAAAAACAAACAGGAAGAAGAAATGTCTGGTCTACCTTAGGGCCCAGGTTTTAGTGTAACAAAGGCTTGTCTCCTCCCAGGACTTACCATGTTCCCCTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,239 SEQUENCE_TARGET=89,45 P3_COMMENT=CL is CTGCAGCCATCAGCATATGT P3_COMMENT=CR is AACCTGGGCCCTAAGGTAGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.191097 PRIMER_LEFT_0_PENALTY=0.146193 PRIMER_RIGHT_0_PENALTY=0.044904 PRIMER_LEFT_0_SEQUENCE=CTGCAGCCATCAGCATATGT PRIMER_RIGHT_0_SEQUENCE=AACCTGGGCCCTAAGGTAGA PRIMER_LEFT_0=41,20 PRIMER_RIGHT_0=208,20 PRIMER_LEFT_0_TM=59.854 PRIMER_RIGHT_0_TM=59.955 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=5.6000 PRIMER_RIGHT_0_END_STABILITY=5.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=168 PRIMER_PAIR_1_PENALTY=0.192939 PRIMER_LEFT_1_PENALTY=0.146193 PRIMER_RIGHT_1_PENALTY=0.046746 PRIMER_LEFT_1_SEQUENCE=CTGCAGCCATCAGCATATGT PRIMER_RIGHT_1_SEQUENCE=CTAAAACCTGGGCCCTAAGG PRIMER_LEFT_1=41,20 PRIMER_RIGHT_1=212,20 PRIMER_LEFT_1_TM=59.854 PRIMER_RIGHT_1_TM=59.953 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=5.6000 PRIMER_RIGHT_1_END_STABILITY=7.5000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=172 PRIMER_PAIR_2_PENALTY=0.316731 PRIMER_LEFT_2_PENALTY=0.146193 PRIMER_RIGHT_2_PENALTY=0.170538 PRIMER_LEFT_2_SEQUENCE=CTGCAGCCATCAGCATATGT PRIMER_RIGHT_2_SEQUENCE=AAAACCTGGGCCCTAAGGTA PRIMER_LEFT_2=41,20 PRIMER_RIGHT_2=210,20 PRIMER_LEFT_2_TM=59.854 PRIMER_RIGHT_2_TM=59.829 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=5.6000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=170 PRIMER_PAIR_3_PENALTY=0.316731 PRIMER_LEFT_3_PENALTY=0.146193 PRIMER_RIGHT_3_PENALTY=0.170538 PRIMER_LEFT_3_SEQUENCE=CTGCAGCCATCAGCATATGT PRIMER_RIGHT_3_SEQUENCE=TAAAACCTGGGCCCTAAGGT PRIMER_LEFT_3=41,20 PRIMER_RIGHT_3=211,20 PRIMER_LEFT_3_TM=59.854 PRIMER_RIGHT_3_TM=59.829 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=5.6000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=171 PRIMER_PAIR_4_PENALTY=0.492707 PRIMER_LEFT_4_PENALTY=0.447804 PRIMER_RIGHT_4_PENALTY=0.044904 PRIMER_LEFT_4_SEQUENCE=CAGCCATCAGCATATGTGGT PRIMER_RIGHT_4_SEQUENCE=AACCTGGGCCCTAAGGTAGA PRIMER_LEFT_4=44,20 PRIMER_RIGHT_4=208,20 PRIMER_LEFT_4_TM=59.552 PRIMER_RIGHT_4_TM=59.955 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=7.6000 PRIMER_RIGHT_4_END_STABILITY=5.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=165 = SEQUENCE_ID=11.RHAP64FD8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCTTAATTAATGCTTACTGACGGAAAAAAGGACAGGGACAATAATCACCTACTGAACAAAGAAAGTGATATGTATGGAAAANTAAAAGCAGATTATTTCACATAATGTAAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATGCTTGCAGCGGGTGCCTATCTTAATCCCAGCAGGCAGACTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,193 SEQUENCE_TARGET=135,30 P3_COMMENT=CL is CTGACGGAAAAAAGGACAGG P3_COMMENT=CR is GTCTGCCTGCTGGGATTAAG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.458353 PRIMER_LEFT_0_PENALTY=0.294694 PRIMER_RIGHT_0_PENALTY=0.163658 PRIMER_LEFT_0_SEQUENCE=CTGACGGAAAAAAGGACAGG PRIMER_RIGHT_0_SEQUENCE=GTCTGCCTGCTGGGATTAAG PRIMER_LEFT_0=38,20 PRIMER_RIGHT_0=210,20 PRIMER_LEFT_0_TM=59.705 PRIMER_RIGHT_0_TM=59.836 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=6.3000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=173 PRIMER_PAIR_1_PENALTY=0.458353 PRIMER_LEFT_1_PENALTY=0.294694 PRIMER_RIGHT_1_PENALTY=0.163658 PRIMER_LEFT_1_SEQUENCE=CTGACGGAAAAAAGGACAGG PRIMER_RIGHT_1_SEQUENCE=AGTCTGCCTGCTGGGATTAA PRIMER_LEFT_1=38,20 PRIMER_RIGHT_1=211,20 PRIMER_LEFT_1_TM=59.705 PRIMER_RIGHT_1_TM=59.836 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=174 PRIMER_PAIR_2_PENALTY=0.504116 PRIMER_LEFT_2_PENALTY=0.340458 PRIMER_RIGHT_2_PENALTY=0.163658 PRIMER_LEFT_2_SEQUENCE=ACGGAAAAAAGGACAGGGAC PRIMER_RIGHT_2_SEQUENCE=GTCTGCCTGCTGGGATTAAG PRIMER_LEFT_2=41,20 PRIMER_RIGHT_2=210,20 PRIMER_LEFT_2_TM=60.340 PRIMER_RIGHT_2_TM=59.836 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=9.1000 PRIMER_RIGHT_2_END_STABILITY=6.3000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=170 PRIMER_PAIR_3_PENALTY=0.504116 PRIMER_LEFT_3_PENALTY=0.340458 PRIMER_RIGHT_3_PENALTY=0.163658 PRIMER_LEFT_3_SEQUENCE=ACGGAAAAAAGGACAGGGAC PRIMER_RIGHT_3_SEQUENCE=AGTCTGCCTGCTGGGATTAA PRIMER_LEFT_3=41,20 PRIMER_RIGHT_3=211,20 PRIMER_LEFT_3_TM=60.340 PRIMER_RIGHT_3_TM=59.836 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=9.1000 PRIMER_RIGHT_3_END_STABILITY=6.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=171 PRIMER_PAIR_4_PENALTY=0.947784 PRIMER_LEFT_4_PENALTY=0.294694 PRIMER_RIGHT_4_PENALTY=0.653090 PRIMER_LEFT_4_SEQUENCE=CTGACGGAAAAAAGGACAGG PRIMER_RIGHT_4_SEQUENCE=GGGATTAAGATAGGCACCCG PRIMER_LEFT_4=38,20 PRIMER_RIGHT_4=199,20 PRIMER_LEFT_4_TM=59.705 PRIMER_RIGHT_4_TM=60.653 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=11.1000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=162 = SEQUENCE_ID=12.RHAP64FD9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNCCACTATATCCCCTGCCTTTGTGGACCTGAGCATCTCATTGACGAATCCCAAATTATACCCCCTTCATTATTCATTAAAGTGTGGTGGGCAGAAATGTGTGTTCACGAATTCAGCTATGGAGAGGTTATAACTGAAGCCTCTGAATGCCCCACAGGGACCAATGTCAGCCAGTTCTATGCCCCTGTTCAGTATCTGCTTGAATCTGAAGCCGGACTCTGTATGTATGTGTATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAAGANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCANATATATATANNTTNTCNNNGGGGGGGGGGGGGNGAGGGAGAANANNNNNNCGGAGGNNNTANNNTGNGCNAGGTGNNNNNNTATNNGNNNACNCNCNTCNNGGGGC SEQUENCE_INCLUDED_REGION=13,445 SEQUENCE_TARGET=246,44 SEQUENCE_TARGET=302,46 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=14.RHAP64FE2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT SEQUENCE_INCLUDED_REGION=19,432 SEQUENCE_TARGET=44,46 SEQUENCE_TARGET=92,33 SEQUENCE_TARGET=140,26 SEQUENCE_TARGET=170,14 SEQUENCE_TARGET=211,20 SEQUENCE_TARGET=238,26 SEQUENCE_TARGET=336,14 P3_COMMENT=CL is CACATGTGCACAGTGGTATGT P3_COMMENT=CR is GCCTGTGATGTATGTATGTGAGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.458561 PRIMER_LEFT_0_PENALTY=1.104149 PRIMER_RIGHT_0_PENALTY=5.354412 PRIMER_LEFT_0_SEQUENCE=TCCACATGTGCACAGTGGTAT PRIMER_RIGHT_0_SEQUENCE=GCCTGTGATGTATGTATGTGAGA PRIMER_LEFT_0=21,21 PRIMER_RIGHT_0=313,23 PRIMER_LEFT_0_TM=59.896 PRIMER_RIGHT_0_TM=57.646 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=43.478 PRIMER_LEFT_0_SELF_ANY=12.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.8000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=293 PRIMER_PAIR_1_PENALTY=8.467518 PRIMER_LEFT_1_PENALTY=3.113106 PRIMER_RIGHT_1_PENALTY=5.354412 PRIMER_LEFT_1_SEQUENCE=CACATGTGCACAGTGGTATGT PRIMER_RIGHT_1_SEQUENCE=GCCTGTGATGTATGTATGTGAGA PRIMER_LEFT_1=23,21 PRIMER_RIGHT_1=313,23 PRIMER_LEFT_1_TM=57.887 PRIMER_RIGHT_1_TM=57.646 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=43.478 PRIMER_LEFT_1_SELF_ANY=10.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=5.6000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=291 PRIMER_PAIR_2_PENALTY=8.789928 PRIMER_LEFT_2_PENALTY=1.104149 PRIMER_RIGHT_2_PENALTY=7.685778 PRIMER_LEFT_2_SEQUENCE=TCCACATGTGCACAGTGGTAT PRIMER_RIGHT_2_SEQUENCE=ATAAATTCATTTTTGCCTGTCATAT PRIMER_LEFT_2=21,21 PRIMER_RIGHT_2=288,25 PRIMER_LEFT_2_TM=59.896 PRIMER_RIGHT_2_TM=57.314 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=24.000 PRIMER_LEFT_2_SELF_ANY=12.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.8000 PRIMER_RIGHT_2_END_STABILITY=5.8000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=268 PRIMER_PAIR_3_PENALTY=9.104196 PRIMER_LEFT_3_PENALTY=3.749784 PRIMER_RIGHT_3_PENALTY=5.354412 PRIMER_LEFT_3_SEQUENCE=CCACATGTGCACAGTGGTATGT PRIMER_RIGHT_3_SEQUENCE=GCCTGTGATGTATGTATGTGAGA PRIMER_LEFT_3=22,22 PRIMER_RIGHT_3=313,23 PRIMER_LEFT_3_TM=61.750 PRIMER_RIGHT_3_TM=57.646 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=12.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=5.6000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=292 PRIMER_PAIR_4_PENALTY=10.198358 PRIMER_LEFT_4_PENALTY=4.843946 PRIMER_RIGHT_4_PENALTY=5.354412 PRIMER_LEFT_4_SEQUENCE=TCCACATGTGCACAGTGGTATG PRIMER_RIGHT_4_SEQUENCE=GCCTGTGATGTATGTATGTGAGA PRIMER_LEFT_4=21,22 PRIMER_RIGHT_4=313,23 PRIMER_LEFT_4_TM=62.844 PRIMER_RIGHT_4_TM=57.646 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=12.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=5.6000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=293 = SEQUENCE_ID=15.RHAP64FE3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCGACGACCTGACTTGGATCTTCTGGAACCACCTGGTAGAAGGGAGAATATGGTTGTCTTCAGACCACTGCAAGCCGTAGTAAATGGATGTAAATATATGTGCACACACAGAGTAATTATAAGTAATGAAAGAAAAGAAGTAAACACTTTCCATAATGTAAAGAAGAAGGCAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTATTCTTCACACAGTGACCCTCTCCACAGAGTCTCTCCTGTGTATTCCGCACACAGTGATCCTCTCCACCACCACCAATGACTTTTCTATAGCACGAATCTAATTCCGCTGGTAAAAGTCCTGGGTTGGCATGCCACAATTTTTCTTGTTCTTTTTAAAAGTAATTTTAGAGTAAATTTACAAAGTAATTNGTATTT SEQUENCE_INCLUDED_REGION=20,420 SEQUENCE_TARGET=194,45 P3_COMMENT=CL is TCCATAATGTAAAGAAGAAGGCAA P3_COMMENT=CR is TGGAGAGGATCACTGTGTGC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.960761 PRIMER_LEFT_0_PENALTY=4.787991 PRIMER_RIGHT_0_PENALTY=0.172769 PRIMER_LEFT_0_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA PRIMER_RIGHT_0_SEQUENCE=TGGAGAGGATCACTGTGTGC PRIMER_LEFT_0=168,24 PRIMER_RIGHT_0=310,20 PRIMER_LEFT_0_TM=59.212 PRIMER_RIGHT_0_TM=59.827 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=143 PRIMER_PAIR_1_PENALTY=4.960761 PRIMER_LEFT_1_PENALTY=4.787991 PRIMER_RIGHT_1_PENALTY=0.172769 PRIMER_LEFT_1_SEQUENCE=TTCCATAATGTAAAGAAGAAGGCA PRIMER_RIGHT_1_SEQUENCE=TGGAGAGGATCACTGTGTGC PRIMER_LEFT_1=167,24 PRIMER_RIGHT_1=310,20 PRIMER_LEFT_1_TM=59.212 PRIMER_RIGHT_1_TM=59.827 PRIMER_LEFT_1_GC_PERCENT=33.333 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=144 PRIMER_PAIR_2_PENALTY=4.963076 PRIMER_LEFT_2_PENALTY=4.787991 PRIMER_RIGHT_2_PENALTY=0.175084 PRIMER_LEFT_2_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA PRIMER_RIGHT_2_SEQUENCE=CTGTGTGCGGAATACACAGG PRIMER_LEFT_2=168,24 PRIMER_RIGHT_2=298,20 PRIMER_LEFT_2_TM=59.212 PRIMER_RIGHT_2_TM=60.175 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=10.0000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=131 PRIMER_PAIR_3_PENALTY=4.963076 PRIMER_LEFT_3_PENALTY=4.787991 PRIMER_RIGHT_3_PENALTY=0.175084 PRIMER_LEFT_3_SEQUENCE=TTCCATAATGTAAAGAAGAAGGCA PRIMER_RIGHT_3_SEQUENCE=CTGTGTGCGGAATACACAGG PRIMER_LEFT_3=167,24 PRIMER_RIGHT_3=298,20 PRIMER_LEFT_3_TM=59.212 PRIMER_RIGHT_3_TM=60.175 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=132 PRIMER_PAIR_4_PENALTY=5.051337 PRIMER_LEFT_4_PENALTY=4.787991 PRIMER_RIGHT_4_PENALTY=0.263346 PRIMER_LEFT_4_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA PRIMER_RIGHT_4_SEQUENCE=TGTGCGGAATACACAGGAGA PRIMER_LEFT_4=168,24 PRIMER_RIGHT_4=295,20 PRIMER_LEFT_4_TM=59.212 PRIMER_RIGHT_4_TM=60.263 PRIMER_LEFT_4_GC_PERCENT=33.333 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=128 = SEQUENCE_ID=15.RHAP64FE9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNCCTGAGCACCAGAAACCACAGAGTTGTGCTATACNNNNTGAGCAATAGCCCAAGCTGCGTGTTAGCTCTGCACAGCATGGTGCTAACTGTGTTGTTTTGTCAATCCCTCACCACTANCTGTGNGTGTGCANNNNNNNNNNNNNNNNNNNNNNNGTGNNCANGNGGGTGAANGTGTATGNGTGNGCNTGCGNNNNNNNNNNNNNNNNNNGCATGNGNNNNNNNNNNNNNNNNNNNTATGNGNATGNGGCGNGAGNGTGTGNATGTGTGGTNTGCATGTATGTGNNNGTNCAATGTGGTANNNTNGTGNGGCANNCNTGNNNGTGTGNTGNTGGNNGTGNTAANNGGTGNAGNGTGNGNGCGGGCGNGNGNTGGTNTNGTGTGNGGTTGNGGNNNNGNGTGTNGNGGNNCNCNNGGANNGCGNNNNNNNNNCCGNGCNGGNTCNNGGNANTANTNGNNNANNGNNNGNANAGNNGAC SEQUENCE_INCLUDED_REGION=13,475 SEQUENCE_TARGET=143,23 SEQUENCE_TARGET=204,15 SEQUENCE_TARGET=229,18 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=16.RHAP64FF7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCATTCTTTGCTATTTATTGTTCCTGGGTAGCATTTCCTAAATACATCCATGAAGTGCTTTTTCTGTAGAAGGTTAACTTGTGTAACATGTGGCAGGACCTAGTACTGAGTGAGTTTGCCTTCACTTCAGTCTCACAAGAACTTGAATAGGCTGGTGTCTAATAATGGAGCTGTTGAAATGTTGGGNTCCTGAACTTTTCAGATTTGTCAATGGACTAGGGTCATGTGAAACACACATTGAAGAGAACTTTCAGTTGGACTNTTNAAGGGTANCCTTTTNNTTTTTAGAGATCTTNATNATTATTTTTTGCCACGNGTACNNNNNNNNNNNNNNNNNACTANGTGCATGTAAGTACAGACGCCTGTGGAAGGTGGAGANTTGGATCCCCTGGAGCTGGAGTTAAAGGCGGTTATAAGTTAGGAACTGAACTGAGGTCCT SEQUENCE_INCLUDED_REGION=23,438 SEQUENCE_TARGET=343,16 P3_COMMENT=CL is TTGTCAATGGACTAGGGTCATG P3_COMMENT=CR is ACCTTCCACAGGCGTCTGTA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.858479 PRIMER_LEFT_0_PENALTY=2.147295 PRIMER_RIGHT_0_PENALTY=0.711184 PRIMER_LEFT_0_SEQUENCE=CAATGGACTAGGGTCATGTGAA PRIMER_RIGHT_0_SEQUENCE=CCTTCCACAGGCGTCTGTAC PRIMER_LEFT_0=230,22 PRIMER_RIGHT_0=393,20 PRIMER_LEFT_0_TM=59.853 PRIMER_RIGHT_0_TM=60.711 PRIMER_LEFT_0_GC_PERCENT=45.455 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=5.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=164 PRIMER_PAIR_1_PENALTY=2.858479 PRIMER_LEFT_1_PENALTY=2.147295 PRIMER_RIGHT_1_PENALTY=0.711184 PRIMER_LEFT_1_SEQUENCE=CAATGGACTAGGGTCATGTGAA PRIMER_RIGHT_1_SEQUENCE=ACCTTCCACAGGCGTCTGTA PRIMER_LEFT_1=230,22 PRIMER_RIGHT_1=394,20 PRIMER_LEFT_1_TM=59.853 PRIMER_RIGHT_1_TM=60.711 PRIMER_LEFT_1_GC_PERCENT=45.455 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=5.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=165 PRIMER_PAIR_2_PENALTY=2.858479 PRIMER_LEFT_2_PENALTY=2.147295 PRIMER_RIGHT_2_PENALTY=0.711184 PRIMER_LEFT_2_SEQUENCE=TTGTCAATGGACTAGGGTCATG PRIMER_RIGHT_2_SEQUENCE=CCTTCCACAGGCGTCTGTAC PRIMER_LEFT_2=226,22 PRIMER_RIGHT_2=393,20 PRIMER_LEFT_2_TM=59.853 PRIMER_RIGHT_2_TM=60.711 PRIMER_LEFT_2_GC_PERCENT=45.455 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=5.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=168 PRIMER_PAIR_3_PENALTY=2.858479 PRIMER_LEFT_3_PENALTY=2.147295 PRIMER_RIGHT_3_PENALTY=0.711184 PRIMER_LEFT_3_SEQUENCE=TTGTCAATGGACTAGGGTCATG PRIMER_RIGHT_3_SEQUENCE=ACCTTCCACAGGCGTCTGTA PRIMER_LEFT_3=226,22 PRIMER_RIGHT_3=394,20 PRIMER_LEFT_3_TM=59.853 PRIMER_RIGHT_3_TM=60.711 PRIMER_LEFT_3_GC_PERCENT=45.455 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=5.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=169 PRIMER_PAIR_4_PENALTY=3.086274 PRIMER_LEFT_4_PENALTY=2.375090 PRIMER_RIGHT_4_PENALTY=0.711184 PRIMER_LEFT_4_SEQUENCE=TCAATGGACTAGGGTCATGTGA PRIMER_RIGHT_4_SEQUENCE=CCTTCCACAGGCGTCTGTAC PRIMER_LEFT_4=229,22 PRIMER_RIGHT_4=393,20 PRIMER_LEFT_4_TM=60.375 PRIMER_RIGHT_4_TM=60.711 PRIMER_LEFT_4_GC_PERCENT=45.455 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=5.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=165 = SEQUENCE_ID=17.RHAP64FF8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCCCAGTCCAAGTTTGCAGTAGTTCCCTAGTTAGGGAATATTTTTCTGATGACATAGGTGTAGTCTCATCACTGTGTGCTTACTGCCTAGATTAGCACAGAGCAAAGAATGAATTGGTTTTCCAGGGAAAATTGCTAAATTAGTAAACACGAGAGTAAGGCAGGAAAGAGCAACAATTTTACTCACAGGTTAATGGTTCACGTTTAGTCAAATTCCAAACTATGGCTCGGTGGTTGGTATAATTATAAGGACATGTTCTATGATTCCATCTCTTTATTAAATATCTCTAATCNAGGGACTGCNAGGAAATGGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGCACTCACGGTGTGCATGTGGANNNNNNNNNNNNNNNNNNNNNNGTGTCTGAAATATNTCTTGTGTGTGCNAGGNG SEQUENCE_INCLUDED_REGION=23,443 SEQUENCE_TARGET=340,48 SEQUENCE_TARGET=412,22 P3_COMMENT=CL is AAACTATGGCTCGGTGGTTG P3_COMMENT=CR is CACATGCACACCGTGAGTG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.849917 PRIMER_LEFT_0_PENALTY=0.006945 PRIMER_RIGHT_0_PENALTY=1.842973 PRIMER_LEFT_0_SEQUENCE=AAACTATGGCTCGGTGGTTG PRIMER_RIGHT_0_SEQUENCE=CACATGCACACCGTGAGTG PRIMER_LEFT_0=239,20 PRIMER_RIGHT_0=409,19 PRIMER_LEFT_0_TM=59.993 PRIMER_RIGHT_0_TM=60.843 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=171 PRIMER_PAIR_1_PENALTY=1.849917 PRIMER_LEFT_1_PENALTY=0.006945 PRIMER_RIGHT_1_PENALTY=1.842973 PRIMER_LEFT_1_SEQUENCE=CAAACTATGGCTCGGTGGTT PRIMER_RIGHT_1_SEQUENCE=CACATGCACACCGTGAGTG PRIMER_LEFT_1=238,20 PRIMER_RIGHT_1=409,19 PRIMER_LEFT_1_TM=59.993 PRIMER_RIGHT_1_TM=60.843 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=172 PRIMER_PAIR_2_PENALTY=2.056478 PRIMER_LEFT_2_PENALTY=0.213506 PRIMER_RIGHT_2_PENALTY=1.842973 PRIMER_LEFT_2_SEQUENCE=ATGGCTCGGTGGTTGGTATA PRIMER_RIGHT_2_SEQUENCE=CACATGCACACCGTGAGTG PRIMER_LEFT_2=244,20 PRIMER_RIGHT_2=409,19 PRIMER_LEFT_2_TM=60.214 PRIMER_RIGHT_2_TM=60.843 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=4.6000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=166 PRIMER_PAIR_3_PENALTY=2.056478 PRIMER_LEFT_3_PENALTY=0.213506 PRIMER_RIGHT_3_PENALTY=1.842973 PRIMER_LEFT_3_SEQUENCE=TATGGCTCGGTGGTTGGTAT PRIMER_RIGHT_3_SEQUENCE=CACATGCACACCGTGAGTG PRIMER_LEFT_3=243,20 PRIMER_RIGHT_3=409,19 PRIMER_LEFT_3_TM=60.214 PRIMER_RIGHT_3_TM=60.843 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=57.895 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.8000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=167 PRIMER_PAIR_4_PENALTY=2.721606 PRIMER_LEFT_4_PENALTY=0.006945 PRIMER_RIGHT_4_PENALTY=2.714661 PRIMER_LEFT_4_SEQUENCE=AAACTATGGCTCGGTGGTTG PRIMER_RIGHT_4_SEQUENCE=CCACATGCACACCGTGAGT PRIMER_LEFT_4=239,20 PRIMER_RIGHT_4=410,19 PRIMER_LEFT_4_TM=59.993 PRIMER_RIGHT_4_TM=61.715 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=57.895 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=172 = SEQUENCE_ID=18.RHAP64FF9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNCCTTGGAAAATCACTGGGAAAGGGCTAAGTTGGCACGCCCCTTAGAGCAGGGAGGAACTNGGTGGTCGGCAGGGTCATTATGACATCTTGTGGTTGTTCTCAAGATTTACCACAGGGAGGGGTAGGGGGGTCTTTCCGAGAACAGTATCTTTGTTTTGTACTAACTTATGGTCAGAATAGTCTGGTCATTCTGACTACTANAACTTTGTCATAAATTAGGCAACCGGAAGTCAGATGTATTTCTCAAAACCTTGTTTTTACAACTTTGTTTCTTTGNNTCNCATATCTATGGAACTATGAAACCTTANTTCNTCAGNGGGGGGGGGGGGGAGAAAGACNNNNNNNNNNNNNNNNNNGAGNGGAGAGAGGANAGNGGANAGACNGANNNAGNNANGANAGACANAGACAGAGNACNGGAAAGAGNNNNNCAAGNNNCAGAAGGNGNGNNNNG SEQUENCE_INCLUDED_REGION=13,451 SEQUENCE_TARGET=351,18 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=19.RHAP64FG1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNCCAGAGAGTGATCTACCTACTGTTGGTTCCAGAGCCTGAGTCTGTTACAGCGGTCTGCACACACCACCATTCCCATTTCCAAGATAAGGAAANTGGGTCAAGGTNTTTGGGTGAGCGTGCGACAGATGAACTCATGGTCAAGGACTTCTTGGCTTTGAAAACCTGACCCTCACTCCCAAGTGTTCTCTGGGCTGGTTTGCCTCAGTTGGTTTTAACAGACTGGTCTAAAATTTCACCTCAAAATATAGAGCATGAAAGTAGAAATAAAGCTTCAATATCTGTCCAAATCTCATTTGTTCTATTTTTTATCATCATCATCATCACCACCACCACCATCATCATCATTACCACCACCATCATCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACNATCACCANCACCATCATCACCACC SEQUENCE_INCLUDED_REGION=18,427 SEQUENCE_TARGET=381,35 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=21.RHAP64FG3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCCAGCCAATCTAACCAGTTAACATGCTCCAGGTTCAGTGAGAGACTTTCAAAAGGAATAAAGCCATAGAGAGGGGCATCCAGTGTTGACCTATGGCATGNNNNNNNNNNNNNNGGCATGTGCACCCATGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGCACTTCATTAGTCAAGTACAGCAGAGTGAACAGGCTAGAAGGTAACAGGTGACCACGTGTACCTCCCTCCACCCCATCCTCCTACCCCCACACACCCTCTGCAGGGAGCTGCCTGGAGAAACAGNAGAGCCTGTGGTAAGGTGTGCTGGGAGGGTGGAGAGTTAAGGAAAAGTGCAGAAAGCGATCTGTGTGGTTTCCACAGAAAGGNATGGCTGCCGTGGAATGTGTCCGGG SEQUENCE_INCLUDED_REGION=21,423 SEQUENCE_TARGET=120,14 SEQUENCE_TARGET=152,55 P3_COMMENT=CL is TCCAGTGTTGACCTATGGCA P3_COMMENT=CR is CACGTGGTCACCTGTTACCTT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.173834 PRIMER_LEFT_0_PENALTY=0.112124 PRIMER_RIGHT_0_PENALTY=1.061709 PRIMER_LEFT_0_SEQUENCE=TCCAGTGTTGACCTATGGCA PRIMER_RIGHT_0_SEQUENCE=CACGTGGTCACCTGTTACCTT PRIMER_LEFT_0=98,20 PRIMER_RIGHT_0=269,21 PRIMER_LEFT_0_TM=60.112 PRIMER_RIGHT_0_TM=59.938 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=52.381 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=172 PRIMER_PAIR_1_PENALTY=1.621151 PRIMER_LEFT_1_PENALTY=0.112124 PRIMER_RIGHT_1_PENALTY=1.509026 PRIMER_LEFT_1_SEQUENCE=TCCAGTGTTGACCTATGGCA PRIMER_RIGHT_1_SEQUENCE=CACGTGGTCACCTGTTACCT PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=269,20 PRIMER_LEFT_1_TM=60.112 PRIMER_RIGHT_1_TM=58.491 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=10.0000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=172 PRIMER_PAIR_2_PENALTY=1.702135 PRIMER_LEFT_2_PENALTY=0.112124 PRIMER_RIGHT_2_PENALTY=1.590010 PRIMER_LEFT_2_SEQUENCE=TCCAGTGTTGACCTATGGCA PRIMER_RIGHT_2_SEQUENCE=ACACGTGGTCACCTGTTACCT PRIMER_LEFT_2=98,20 PRIMER_RIGHT_2=270,21 PRIMER_LEFT_2_TM=60.112 PRIMER_RIGHT_2_TM=59.410 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=10.0000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=173 PRIMER_PAIR_3_PENALTY=1.779345 PRIMER_LEFT_3_PENALTY=0.112124 PRIMER_RIGHT_3_PENALTY=1.667221 PRIMER_LEFT_3_SEQUENCE=TCCAGTGTTGACCTATGGCA PRIMER_RIGHT_3_SEQUENCE=ACACGTGGTCACCTGTTACC PRIMER_LEFT_3=98,20 PRIMER_RIGHT_3=270,20 PRIMER_LEFT_3_TM=60.112 PRIMER_RIGHT_3_TM=58.333 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=10.0000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=173 PRIMER_PAIR_4_PENALTY=1.989876 PRIMER_LEFT_4_PENALTY=0.112124 PRIMER_RIGHT_4_PENALTY=1.877751 PRIMER_LEFT_4_SEQUENCE=TCCAGTGTTGACCTATGGCA PRIMER_RIGHT_4_SEQUENCE=CGTGGTCACCTGTTACCTTCT PRIMER_LEFT_4=98,20 PRIMER_RIGHT_4=267,21 PRIMER_LEFT_4_TM=60.112 PRIMER_RIGHT_4_TM=59.122 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=170 = SEQUENCE_ID=22.RHAP64FH7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCACCCCTTGTTGTCTTTACTATGNGCCTCTATATGACTATACGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNTNNNTCNCTCTCNTNNNNTNNANANANANNNNNNNNNNNNNNNNNNNNNTGNGNNGNNNGTGTGNGNGTNNNTCNNTCNNATNCNNNANNGCCANNTGGANTANNNNANNNTCNTNNNAANTAGANNNGGGNNNTNGNANTNNCCNGGAGGTNTCNCANCCACNNCTCNGCANANGNACANNTANNCNNCAGNACTCACATNCTNNGANANACNNNTNTGTTCTGGNNNCCANTNNNAANGNTNNNTTNNNNATAATNNNNNGNNCTCNNCANNGTGNNNNTCNNACTCCNNNNTANGNNTNNNGNTTNCAGNNGNANNNNNAGTANANGNNACNTCNNGNNTNNTGATGGNNNNNNNNNTNTNNGNNNCCNNNTNCNGANNTNNNNNNNNTNG SEQUENCE_INCLUDED_REGION=12,488 SEQUENCE_TARGET=57,22 SEQUENCE_TARGET=137,18 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=26.RHAP64FA11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCATCCTACTAAGGGAAAGCAAAAAAAAAACTTCCTCCTGACTNTGATGTTGAAAGAAGAATGAAACGAACATCTCTCCAACAATATCTAATCAGCAGGAGTCATACTCACCCTTAGGTCTAGATTTACACTTTTCCAAGATCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGGGGGGGTGGAGAGGAAGAAGGACAGAGACACAGAGAGATCACTAGACATATAATACACTACGAATTGTACAATTAAGAATTCAAAAAATTAAAAAAAAAACCATTTCTGATAGCCAGACAAGTCAGTTTGATGAACAAAGAGGAAGAATGTGAGTGAGGAAGGATGNAATTGGAGAGAGGGATTGAAAATGGCAGTANTGTTAAAGATTGGAGAAGAAGGAAGTCCTGTGGGAGAGGGGGTGGGGGAGA SEQUENCE_INCLUDED_REGION=12,445 SEQUENCE_TARGET=157,46 P3_COMMENT=CL is CAGCAGGAGTCATACTCACCC P3_COMMENT=CR is GTCCTTCTTCCTCTCCACCC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.314269 PRIMER_LEFT_0_PENALTY=1.263803 PRIMER_RIGHT_0_PENALTY=0.050466 PRIMER_LEFT_0_SEQUENCE=CAGCAGGAGTCATACTCACCC PRIMER_RIGHT_0_SEQUENCE=GTCCTTCTTCCTCTCCACCC PRIMER_LEFT_0=103,21 PRIMER_RIGHT_0=230,20 PRIMER_LEFT_0_TM=59.736 PRIMER_RIGHT_0_TM=60.050 PRIMER_LEFT_0_GC_PERCENT=57.143 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=128 PRIMER_PAIR_1_PENALTY=2.035901 PRIMER_LEFT_1_PENALTY=1.263803 PRIMER_RIGHT_1_PENALTY=0.772097 PRIMER_LEFT_1_SEQUENCE=CAGCAGGAGTCATACTCACCC PRIMER_RIGHT_1_SEQUENCE=TGTCCTTCTTCCTCTCCACC PRIMER_LEFT_1=103,21 PRIMER_RIGHT_1=231,20 PRIMER_LEFT_1_TM=59.736 PRIMER_RIGHT_1_TM=59.228 PRIMER_LEFT_1_GC_PERCENT=57.143 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=129 PRIMER_PAIR_2_PENALTY=2.501080 PRIMER_LEFT_2_PENALTY=1.263803 PRIMER_RIGHT_2_PENALTY=1.237277 PRIMER_LEFT_2_SEQUENCE=CAGCAGGAGTCATACTCACCC PRIMER_RIGHT_2_SEQUENCE=CTGTCCTTCTTCCTCTCCACC PRIMER_LEFT_2=103,21 PRIMER_RIGHT_2=232,21 PRIMER_LEFT_2_TM=59.736 PRIMER_RIGHT_2_TM=60.237 PRIMER_LEFT_2_GC_PERCENT=57.143 PRIMER_RIGHT_2_GC_PERCENT=57.143 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=130 PRIMER_PAIR_3_PENALTY=2.718781 PRIMER_LEFT_3_PENALTY=2.668315 PRIMER_RIGHT_3_PENALTY=0.050466 PRIMER_LEFT_3_SEQUENCE=TGAAACGAACATCTCTCCAACA PRIMER_RIGHT_3_SEQUENCE=GTCCTTCTTCCTCTCCACCC PRIMER_LEFT_3=72,22 PRIMER_RIGHT_3=230,20 PRIMER_LEFT_3_TM=60.668 PRIMER_RIGHT_3_TM=60.050 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=159 PRIMER_PAIR_4_PENALTY=2.751591 PRIMER_LEFT_4_PENALTY=2.701125 PRIMER_RIGHT_4_PENALTY=0.050466 PRIMER_LEFT_4_SEQUENCE=CAGCAGGAGTCATACTCACCCT PRIMER_RIGHT_4_SEQUENCE=GTCCTTCTTCCTCTCCACCC PRIMER_LEFT_4=103,22 PRIMER_RIGHT_4=230,20 PRIMER_LEFT_4_TM=60.701 PRIMER_RIGHT_4_TM=60.050 PRIMER_LEFT_4_GC_PERCENT=54.545 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=9.1000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=128 = SEQUENCE_ID=30.RHAP64FB12.seq SEQUENCE_TEMPLATE=CCTTCCTATTTTCCTCTCCAGTGCTGAGACCAAAGTGAGATCAAAGTCATGCATNACCATGGGNACCTTCCTTTTCANNANTTTNTTTCATCNTAGGAAGTANGTANANANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTTAAAATCAGCACTCAAGANNTNTNNGNTTCCTATCCCTATANCANNCCCCCCAAAACANCCNNAAAGCCACCNGCNCCAGAGAAAGAGGGAGATGAAATCTCTAGTNNGAAAAANNAGNNGAGGGAGATNACAGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGNANANACACAGATACANNNCCNNANANNNNGGNGANACNNNNNNNNNGNNGCNNGCGNGCNNNGAGTANTNNANAAANANNGNTNCTNNNNGNGNNNCNNGGNGNNNA SEQUENCE_INCLUDED_REGION=0,451 SEQUENCE_TARGET=113,32 SEQUENCE_TARGET=146,26 SEQUENCE_TARGET=314,24 P3_COMMENT=CL is TCTCCAGTGCTGAGACCAAA P3_COMMENT=CR is TTTCATCTCCCTCTTTCTCTGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.634023 PRIMER_LEFT_0_PENALTY=0.454603 PRIMER_RIGHT_0_PENALTY=2.179421 PRIMER_LEFT_0_SEQUENCE=TCTCCAGTGCTGAGACCAAA PRIMER_RIGHT_0_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG PRIMER_LEFT_0=14,20 PRIMER_RIGHT_0=273,22 PRIMER_LEFT_0_TM=59.545 PRIMER_RIGHT_0_TM=59.821 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=260 PRIMER_PAIR_1_PENALTY=2.718057 PRIMER_LEFT_1_PENALTY=0.538636 PRIMER_RIGHT_1_PENALTY=2.179421 PRIMER_LEFT_1_SEQUENCE=CCAGTGCTGAGACCAAAGTG PRIMER_RIGHT_1_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG PRIMER_LEFT_1=17,20 PRIMER_RIGHT_1=273,22 PRIMER_LEFT_1_TM=59.461 PRIMER_RIGHT_1_TM=59.821 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=257 PRIMER_PAIR_2_PENALTY=2.729191 PRIMER_LEFT_2_PENALTY=0.549770 PRIMER_RIGHT_2_PENALTY=2.179421 PRIMER_LEFT_2_SEQUENCE=TCCTCTCCAGTGCTGAGACC PRIMER_RIGHT_2_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG PRIMER_LEFT_2=11,20 PRIMER_RIGHT_2=273,22 PRIMER_LEFT_2_TM=60.550 PRIMER_RIGHT_2_TM=59.821 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.6000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=263 PRIMER_PAIR_3_PENALTY=2.996036 PRIMER_LEFT_3_PENALTY=0.454603 PRIMER_RIGHT_3_PENALTY=2.541433 PRIMER_LEFT_3_SEQUENCE=TCTCCAGTGCTGAGACCAAA PRIMER_RIGHT_3_SEQUENCE=TTCATCTCCCTCTTTCTCTGG PRIMER_LEFT_3=14,20 PRIMER_RIGHT_3=272,21 PRIMER_LEFT_3_TM=59.545 PRIMER_RIGHT_3_TM=58.459 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.8000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=259 PRIMER_PAIR_4_PENALTY=3.054883 PRIMER_LEFT_4_PENALTY=0.875462 PRIMER_RIGHT_4_PENALTY=2.179421 PRIMER_LEFT_4_SEQUENCE=CTCTCCAGTGCTGAGACCAA PRIMER_RIGHT_4_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG PRIMER_LEFT_4=13,20 PRIMER_RIGHT_4=273,22 PRIMER_LEFT_4_TM=59.125 PRIMER_RIGHT_4_TM=59.821 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.455 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=261 = SEQUENCE_ID=31.RHAP64FC10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNCCTAACCCTTTATGCTATCTAGTTACTTCCCAGACATGCACACACGCACGCACGCACGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGCCCCAAGTTACTTGCATTTAGTCTCACCTTGGATACATTCAGCTGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=24,167 SEQUENCE_TARGET=86,52 P3_COMMENT=CL is CTTCCCAGACATGCACACAC P3_COMMENT=CR is CCCACAGCTGAATGTATCCA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.640953 PRIMER_LEFT_0_PENALTY=0.162457 PRIMER_RIGHT_0_PENALTY=0.478496 PRIMER_LEFT_0_SEQUENCE=CTTCCCAGACATGCACACAC PRIMER_RIGHT_0_SEQUENCE=CCCACAGCTGAATGTATCCA PRIMER_LEFT_0=49,20 PRIMER_RIGHT_0=190,20 PRIMER_LEFT_0_TM=60.162 PRIMER_RIGHT_0_TM=59.522 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=142 PRIMER_PAIR_1_PENALTY=0.640953 PRIMER_LEFT_1_PENALTY=0.162457 PRIMER_RIGHT_1_PENALTY=0.478496 PRIMER_LEFT_1_SEQUENCE=ACTTCCCAGACATGCACACA PRIMER_RIGHT_1_SEQUENCE=CCCACAGCTGAATGTATCCA PRIMER_LEFT_1=48,20 PRIMER_RIGHT_1=190,20 PRIMER_LEFT_1_TM=60.162 PRIMER_RIGHT_1_TM=59.522 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=143 PRIMER_PAIR_2_PENALTY=1.645939 PRIMER_LEFT_2_PENALTY=1.167443 PRIMER_RIGHT_2_PENALTY=0.478496 PRIMER_LEFT_2_SEQUENCE=TACTTCCCAGACATGCACACA PRIMER_RIGHT_2_SEQUENCE=CCCACAGCTGAATGTATCCA PRIMER_LEFT_2=47,21 PRIMER_RIGHT_2=190,20 PRIMER_LEFT_2_TM=60.167 PRIMER_RIGHT_2_TM=59.522 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=8.1000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=144 PRIMER_PAIR_3_PENALTY=2.061262 PRIMER_LEFT_3_PENALTY=0.162457 PRIMER_RIGHT_3_PENALTY=1.898804 PRIMER_LEFT_3_SEQUENCE=CTTCCCAGACATGCACACAC PRIMER_RIGHT_3_SEQUENCE=CCCACAGCTGAATGTATCCAA PRIMER_LEFT_3=49,20 PRIMER_RIGHT_3=190,21 PRIMER_LEFT_3_TM=60.162 PRIMER_RIGHT_3_TM=60.899 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=142 PRIMER_PAIR_4_PENALTY=2.061262 PRIMER_LEFT_4_PENALTY=0.162457 PRIMER_RIGHT_4_PENALTY=1.898804 PRIMER_LEFT_4_SEQUENCE=ACTTCCCAGACATGCACACA PRIMER_RIGHT_4_SEQUENCE=CCCACAGCTGAATGTATCCAA PRIMER_LEFT_4=48,20 PRIMER_RIGHT_4=190,21 PRIMER_LEFT_4_TM=60.162 PRIMER_RIGHT_4_TM=60.899 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=143 = SEQUENCE_ID=31.RHAP64FC4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCTGTTGGAGCCCAGCAAAGATGTTTTCTAGAATTCCCAACTCAGACAATCCTTGGAGGTTGTTATGTGAATGTATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACACAGGCTCATGCCTTTGTGTGGAGGTCAGAGGCATCATCTAGCTTACATTTATTAAGACAGCGTTTCACATTTTGCTTGGAATTCATTGATTCAGCTGGACTGGCTGTTTGGAGAGCCTCAGGGATCCATCCACCTGCCTCTGTCCCCTTGACCCTGGGATTACAAGCAGTCATACCCAAACTCAGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAACTCAGAACTTCATCTTGTGTAGCAACTGTTTTTCTAAGCGAGATGTCT SEQUENCE_INCLUDED_REGION=20,416 SEQUENCE_TARGET=99,48 SEQUENCE_TARGET=340,44 P3_COMMENT=CL is AGCCCAGCAAAGATGTTTTC P3_COMMENT=CR is ATGATGCCTCTGACCTCCAC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.763816 PRIMER_LEFT_0_PENALTY=0.683672 PRIMER_RIGHT_0_PENALTY=0.080144 PRIMER_LEFT_0_SEQUENCE=AGCCCAGCAAAGATGTTTTC PRIMER_RIGHT_0_SEQUENCE=ATGATGCCTCTGACCTCCAC PRIMER_LEFT_0=28,20 PRIMER_RIGHT_0=189,20 PRIMER_LEFT_0_TM=59.316 PRIMER_RIGHT_0_TM=60.080 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=162 PRIMER_PAIR_1_PENALTY=0.763816 PRIMER_LEFT_1_PENALTY=0.683672 PRIMER_RIGHT_1_PENALTY=0.080144 PRIMER_LEFT_1_SEQUENCE=GAGCCCAGCAAAGATGTTTT PRIMER_RIGHT_1_SEQUENCE=ATGATGCCTCTGACCTCCAC PRIMER_LEFT_1=27,20 PRIMER_RIGHT_1=189,20 PRIMER_LEFT_1_TM=59.316 PRIMER_RIGHT_1_TM=60.080 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=163 PRIMER_PAIR_2_PENALTY=0.819572 PRIMER_LEFT_2_PENALTY=0.683672 PRIMER_RIGHT_2_PENALTY=0.135900 PRIMER_LEFT_2_SEQUENCE=AGCCCAGCAAAGATGTTTTC PRIMER_RIGHT_2_SEQUENCE=CAAAGGCATGAGCCTGTGTA PRIMER_LEFT_2=28,20 PRIMER_RIGHT_2=168,20 PRIMER_LEFT_2_TM=59.316 PRIMER_RIGHT_2_TM=59.864 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=5.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=141 PRIMER_PAIR_3_PENALTY=0.819572 PRIMER_LEFT_3_PENALTY=0.683672 PRIMER_RIGHT_3_PENALTY=0.135900 PRIMER_LEFT_3_SEQUENCE=GAGCCCAGCAAAGATGTTTT PRIMER_RIGHT_3_SEQUENCE=CAAAGGCATGAGCCTGTGTA PRIMER_LEFT_3=27,20 PRIMER_RIGHT_3=168,20 PRIMER_LEFT_3_TM=59.316 PRIMER_RIGHT_3_TM=59.864 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=5.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=142 PRIMER_PAIR_4_PENALTY=1.176036 PRIMER_LEFT_4_PENALTY=1.095892 PRIMER_RIGHT_4_PENALTY=0.080144 PRIMER_LEFT_4_SEQUENCE=TCAGACAATCCTTGGAGGTTG PRIMER_RIGHT_4_SEQUENCE=ATGATGCCTCTGACCTCCAC PRIMER_LEFT_4=61,21 PRIMER_RIGHT_4=189,20 PRIMER_LEFT_4_TM=60.096 PRIMER_RIGHT_4_TM=60.080 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=129 = SEQUENCE_ID=33.RHAP64FC6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCCAGAACAATGTCATCTGGACAGCTTGCAAAAATGTTAGCATCTGTTCTTGGTCTACCAATGCAAGTCTATGTGGTGAAGGTGTTTGTTATTTACAGTTTAGGAAATAAGGAATAAATCTTGAGATCATCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACTGGAAGTTATTGGTAAAGNGCACAGAGGCTTTCTTTTCTTTTCTTTGTTAANTTCTTTTCAAAAAGGATAAAAAAATATCTTGTCAAACTTAAAACAGCTTATGAATTCTCCCCACTTGCTTCCCAACGTACTGTTTTCTCTGTAATTATACTGAATAACAAAGATTCTATATTTGTCNCATAGTTCTNGCTACTTAC SEQUENCE_INCLUDED_REGION=17,428 SEQUENCE_TARGET=149,40 SEQUENCE_TARGET=190,54 P3_COMMENT=CL is AAGGAATAAATCTTGAGATCATCG P3_COMMENT=CR is AAAGAAAAGAAAGCCTCTGTGC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=8.904598 PRIMER_LEFT_0_PENALTY=6.104219 PRIMER_RIGHT_0_PENALTY=2.800379 PRIMER_LEFT_0_SEQUENCE=AAGGAATAAATCTTGAGATCATCG PRIMER_RIGHT_0_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC PRIMER_LEFT_0=124,24 PRIMER_RIGHT_0=287,22 PRIMER_LEFT_0_TM=57.896 PRIMER_RIGHT_0_TM=59.200 PRIMER_LEFT_0_GC_PERCENT=33.333 PRIMER_RIGHT_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.6000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=164 PRIMER_PAIR_1_PENALTY=9.137284 PRIMER_LEFT_1_PENALTY=6.336905 PRIMER_RIGHT_1_PENALTY=2.800379 PRIMER_LEFT_1_SEQUENCE=AAGGAATAAATCTTGAGATCATCGT PRIMER_RIGHT_1_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC PRIMER_LEFT_1=124,25 PRIMER_RIGHT_1=287,22 PRIMER_LEFT_1_TM=58.663 PRIMER_RIGHT_1_TM=59.200 PRIMER_LEFT_1_GC_PERCENT=32.000 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.0000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=164 PRIMER_PAIR_2_PENALTY=9.224558 PRIMER_LEFT_2_PENALTY=6.104219 PRIMER_RIGHT_2_PENALTY=3.120339 PRIMER_LEFT_2_SEQUENCE=AAGGAATAAATCTTGAGATCATCG PRIMER_RIGHT_2_SEQUENCE=AAGAAAAGAAAGCCTCTGTGC PRIMER_LEFT_2=124,24 PRIMER_RIGHT_2=286,21 PRIMER_LEFT_2_TM=57.896 PRIMER_RIGHT_2_TM=57.880 PRIMER_LEFT_2_GC_PERCENT=33.333 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.6000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=163 PRIMER_PAIR_3_PENALTY=9.382665 PRIMER_LEFT_3_PENALTY=6.582286 PRIMER_RIGHT_3_PENALTY=2.800379 PRIMER_LEFT_3_SEQUENCE=AGGAATAAATCTTGAGATCATCGT PRIMER_RIGHT_3_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC PRIMER_LEFT_3=125,24 PRIMER_RIGHT_3=287,22 PRIMER_LEFT_3_TM=57.418 PRIMER_RIGHT_3_TM=59.200 PRIMER_LEFT_3_GC_PERCENT=33.333 PRIMER_RIGHT_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.0000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=163 PRIMER_PAIR_4_PENALTY=9.457244 PRIMER_LEFT_4_PENALTY=6.336905 PRIMER_RIGHT_4_PENALTY=3.120339 PRIMER_LEFT_4_SEQUENCE=AAGGAATAAATCTTGAGATCATCGT PRIMER_RIGHT_4_SEQUENCE=AAGAAAAGAAAGCCTCTGTGC PRIMER_LEFT_4=124,25 PRIMER_RIGHT_4=286,21 PRIMER_LEFT_4_TM=58.663 PRIMER_RIGHT_4_TM=57.880 PRIMER_LEFT_4_GC_PERCENT=32.000 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.0000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=163 = SEQUENCE_ID=34.RHAP64FD10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNCACTAGGGAGCCTTTATCAAATAAGTAGCTTGACCAAATGCCTTTTCAGTACNNNNNNNNNNNNNNNGCCTTCTCTTTTCTGTATCGGACTATACTTCCCTGAGAAATTCAGAAGAAATTGAAAGGTGAACTACTTCACCATCTCCCCTCTGCGTGCATCCATCTGTTACAAAGGATATCGTTTGACATATATAACCATGTTAACCTTCACGATTTTAAGGAAGTCCTTGGTGGAGGACCACAAAGAATAGATTGACCTCAAAAGGAGGTCTATTGGTCTGAAAAGTTGGCACCCCCAAGTCCTGTGGCTGGGTACCCAAGGATACAAGAAAAATCACCTCGAAGGGTCTGAAAATCAGTTTTGTAGTTAGGAGGAAGAGCNTGGTAAACTGCTCTTCAAAACTACCTGATAGGGGAANATGAGTCCATTGTCTGGTTTGTTT SEQUENCE_INCLUDED_REGION=24,443 SEQUENCE_TARGET=76,15 P3_COMMENT=CL is GCTTGACCAAATGCCTTTTC P3_COMMENT=CR is GTAACAGATGGATGCACGCA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.006754 PRIMER_LEFT_0_PENALTY=0.309288 PRIMER_RIGHT_0_PENALTY=0.697466 PRIMER_LEFT_0_SEQUENCE=GCTTGACCAAATGCCTTTTC PRIMER_RIGHT_0_SEQUENCE=GTAACAGATGGATGCACGCA PRIMER_LEFT_0=51,20 PRIMER_RIGHT_0=193,20 PRIMER_LEFT_0_TM=59.691 PRIMER_RIGHT_0_TM=60.697 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=9.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=143 PRIMER_PAIR_1_PENALTY=1.006754 PRIMER_LEFT_1_PENALTY=0.309288 PRIMER_RIGHT_1_PENALTY=0.697466 PRIMER_LEFT_1_SEQUENCE=GCTTGACCAAATGCCTTTTC PRIMER_RIGHT_1_SEQUENCE=TGTAACAGATGGATGCACGC PRIMER_LEFT_1=51,20 PRIMER_RIGHT_1=194,20 PRIMER_LEFT_1_TM=59.691 PRIMER_RIGHT_1_TM=60.697 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=9.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=144 PRIMER_PAIR_2_PENALTY=1.142819 PRIMER_LEFT_2_PENALTY=0.309288 PRIMER_RIGHT_2_PENALTY=0.833530 PRIMER_LEFT_2_SEQUENCE=GCTTGACCAAATGCCTTTTC PRIMER_RIGHT_2_SEQUENCE=TAACAGATGGATGCACGCAG PRIMER_LEFT_2=51,20 PRIMER_RIGHT_2=192,20 PRIMER_LEFT_2_TM=59.691 PRIMER_RIGHT_2_TM=60.834 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=10.2000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=142 PRIMER_PAIR_3_PENALTY=1.208202 PRIMER_LEFT_3_PENALTY=0.309288 PRIMER_RIGHT_3_PENALTY=0.898913 PRIMER_LEFT_3_SEQUENCE=GCTTGACCAAATGCCTTTTC PRIMER_RIGHT_3_SEQUENCE=CAGAGGGGAGATGGTGAAGT PRIMER_LEFT_3=51,20 PRIMER_RIGHT_3=175,20 PRIMER_LEFT_3_TM=59.691 PRIMER_RIGHT_3_TM=59.101 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=125 PRIMER_PAIR_4_PENALTY=1.510507 PRIMER_LEFT_4_PENALTY=0.309288 PRIMER_RIGHT_4_PENALTY=1.201218 PRIMER_LEFT_4_SEQUENCE=GCTTGACCAAATGCCTTTTC PRIMER_RIGHT_4_SEQUENCE=TAACAGATGGATGCACGCA PRIMER_LEFT_4=51,20 PRIMER_RIGHT_4=192,19 PRIMER_LEFT_4_TM=59.691 PRIMER_RIGHT_4_TM=59.799 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=47.368 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=9.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=142 = SEQUENCE_ID=35.RHAP64FD11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCTCCTGAGAAATGCAGGTCAGGGTACCTTGGCAGGGAATCCCAAGGATCAGTACATGGCAGCTGAAAGTTGTCTTGGATGCAAGACTGGGAGTGAGGCTCAAGTCAGCCGAGCCTCAGCTCTGGATTCCTGAAACAGAATACCAGAATATGTTAGAGAGCGAGGAGACTAAACTAAACATCTGGGAGTCCTCAGCAGATTGTACAGATCCAGGGCTGAGGGNTCATAAGTATGTTGGTCTTTGTGGGGGTGGGAAAGGAACACTTATCTAGATCCCCACCTCCTTACTATGTGCTACTACGGGTGGNGGCAATGCCTTAAGGACCAAGGTTTTTTGTTTTGTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGTGTGTGNGTTGCATNNAATGCANGGNGCAGGTGGTANTAATTGNNGGGGGNTAANNCANTGTTCTTCNTCTACCNNNTTCC SEQUENCE_INCLUDED_REGION=23,460 SEQUENCE_TARGET=369,28 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=36.RHAP64FD6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCACATTTTTCTTTANGNNNNNNNANTGNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCGNNAAGGCACAANTAACCAAGGANGGAGGTCAGACAACAAGTTTATGGCGTCAATTCNCTCTCACCTTTACATGGATNGCCNGNNNTACACTGCAAACATCCGGCACTGNACCGTTCTGCCAGTCCANGGTCTCCCNANNTTTGTCCCCCTCNCTGTCTCNCNGTCTCTGCCCTTGNTAGTCTGNCGNNNCTCCGATANCCTCNNNACCCCNGGNNTTCTGCTCTCNGTGACTTCCCCCTCNCCCTGNCTCCCNCNGTCNGNTCCGTCTNGTCCNTGCNNTTNCCTAGTTTCNCNCCNNTNNGTNTCCCNGGNNTGGCGNGGTCNGNNNGTGCCNCCNNNTATNGTGA SEQUENCE_INCLUDED_REGION=27,434 SEQUENCE_TARGET=57,46 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=37.RHAP64FE4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCCAGCCTTTGATACAGGAACAGGAGGACCCAGTCCGTAAAACCAAAGGTGAGGGAGAAACTAAGACATTCTCAGATAAAGAAACTAAGGAAATGTCTTGATAATGTAACAAGCCTTGGAAACAGCTTTGGCTACAAAGCACCGCTTCCTCTCATGGAACCCACAGCAATCGTTGGCAAGGGCAGTGAGACAGACGGCTCAGCAGTTCTAAGTACTCGGTGACCTGAATTTAACCCACATGGAAGACGGCAACAATCAACCCTTGAAAGTCTTCTGAGTGTGTACCCTGGCACCAAAGGACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTAGGTTTTTCTTTTTTCTTTTTCTTTTTTTAAAGTAGAAGCAGGCAGCCGGAAGTGGTANCTCACAATCTTTATCTTTT SEQUENCE_INCLUDED_REGION=20,428 SEQUENCE_TARGET=322,44 P3_COMMENT=CL is TCTGAGTGTGTACCCTGGCA P3_COMMENT=CR is CGGCTGCCTGCTTCTACTT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.992996 PRIMER_LEFT_0_PENALTY=0.309402 PRIMER_RIGHT_0_PENALTY=1.683594 PRIMER_LEFT_0_SEQUENCE=TCTGAGTGTGTACCCTGGCA PRIMER_RIGHT_0_SEQUENCE=CGGCTGCCTGCTTCTACTT PRIMER_LEFT_0=291,20 PRIMER_RIGHT_0=418,19 PRIMER_LEFT_0_TM=60.309 PRIMER_RIGHT_0_TM=60.684 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=10.0000 PRIMER_RIGHT_0_END_STABILITY=5.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=128 PRIMER_PAIR_1_PENALTY=2.067393 PRIMER_LEFT_1_PENALTY=0.383798 PRIMER_RIGHT_1_PENALTY=1.683594 PRIMER_LEFT_1_SEQUENCE=ACATGGAAGACGGCAACAAT PRIMER_RIGHT_1_SEQUENCE=CGGCTGCCTGCTTCTACTT PRIMER_LEFT_1=255,20 PRIMER_RIGHT_1=418,19 PRIMER_LEFT_1_TM=60.384 PRIMER_RIGHT_1_TM=60.684 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=5.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=164 PRIMER_PAIR_2_PENALTY=2.187940 PRIMER_LEFT_2_PENALTY=0.504346 PRIMER_RIGHT_2_PENALTY=1.683594 PRIMER_LEFT_2_SEQUENCE=GAAGACGGCAACAATCAACC PRIMER_RIGHT_2_SEQUENCE=CGGCTGCCTGCTTCTACTT PRIMER_LEFT_2=260,20 PRIMER_RIGHT_2=418,19 PRIMER_LEFT_2_TM=60.504 PRIMER_RIGHT_2_TM=60.684 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=5.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=159 PRIMER_PAIR_3_PENALTY=2.187940 PRIMER_LEFT_3_PENALTY=0.504346 PRIMER_RIGHT_3_PENALTY=1.683594 PRIMER_LEFT_3_SEQUENCE=GGAAGACGGCAACAATCAAC PRIMER_RIGHT_3_SEQUENCE=CGGCTGCCTGCTTCTACTT PRIMER_LEFT_3=259,20 PRIMER_RIGHT_3=418,19 PRIMER_LEFT_3_TM=60.504 PRIMER_RIGHT_3_TM=60.684 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=57.895 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=5.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=160 PRIMER_PAIR_4_PENALTY=2.238002 PRIMER_LEFT_4_PENALTY=0.309402 PRIMER_RIGHT_4_PENALTY=1.928601 PRIMER_LEFT_4_SEQUENCE=TCTGAGTGTGTACCCTGGCA PRIMER_RIGHT_4_SEQUENCE=CGGCTGCCTGCTTCTACTTT PRIMER_LEFT_4=291,20 PRIMER_RIGHT_4=418,20 PRIMER_LEFT_4_TM=60.309 PRIMER_RIGHT_4_TM=61.929 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=128 = SEQUENCE_ID=38.RHAP64FE5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCATGTAGAATGTGTTTGTTTGTTTTTGTAAACTTAACTTAATCAAGGATATTCCTCACAGGCATACTCAGAAGATAATCTACAAAATCCCTCACATGATGTAGACANGTGGTCTACATCAGTAGGTCTCCACACTCTAGTGGCTGTTTTCTCCAGGTAGTGCTTAACTCTGGCTTTTAGCTGTTTCTAAATATTTCCTGGGAAAATTAAGGTTAACGAGAAACATATTGACCTTCGNTGTTCTTCCTGTGTGGATAATCTGACTTTGGACAGACCTAGGTTGTGAGGAATGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTGCGCGTGCCAGAGGTCGACCACAGCACAGGTGTTCTTTCTCAGAAGCAATCTACCNTATTTTATTTTATNC SEQUENCE_INCLUDED_REGION=17,424 SEQUENCE_TARGET=310,56 P3_COMMENT=CL is CCTAGGTTGTGAGGAATGCC P3_COMMENT=CR is TGCTTCTGAGAAAGAACACCTG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.793510 PRIMER_LEFT_0_PENALTY=0.449829 PRIMER_RIGHT_0_PENALTY=2.343680 PRIMER_LEFT_0_SEQUENCE=CCTAGGTTGTGAGGAATGCC PRIMER_RIGHT_0_SEQUENCE=TGCTTCTGAGAAAGAACACCTG PRIMER_LEFT_0=290,20 PRIMER_RIGHT_0=417,22 PRIMER_LEFT_0_TM=59.550 PRIMER_RIGHT_0_TM=59.656 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=7.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=9.6000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=128 PRIMER_PAIR_1_PENALTY=2.929880 PRIMER_LEFT_1_PENALTY=0.449829 PRIMER_RIGHT_1_PENALTY=2.480050 PRIMER_LEFT_1_SEQUENCE=CCTAGGTTGTGAGGAATGCC PRIMER_RIGHT_1_SEQUENCE=TTCTGAGAAAGAACACCTGTGC PRIMER_LEFT_1=290,20 PRIMER_RIGHT_1=414,22 PRIMER_LEFT_1_TM=59.550 PRIMER_RIGHT_1_TM=59.520 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=125 PRIMER_PAIR_2_PENALTY=3.566148 PRIMER_LEFT_2_PENALTY=2.343332 PRIMER_RIGHT_2_PENALTY=1.222816 PRIMER_LEFT_2_SEQUENCE=GGACAGACCTAGGTTGTGAGG PRIMER_RIGHT_2_SEQUENCE=AAAGAACACCTGTGCTGTGG PRIMER_LEFT_2=283,21 PRIMER_RIGHT_2=407,20 PRIMER_LEFT_2_TM=58.657 PRIMER_RIGHT_2_TM=58.777 PRIMER_LEFT_2_GC_PERCENT=57.143 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=10.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=125 PRIMER_PAIR_3_PENALTY=3.579314 PRIMER_LEFT_3_PENALTY=2.366796 PRIMER_RIGHT_3_PENALTY=1.212518 PRIMER_LEFT_3_SEQUENCE=TTTGGACAGACCTAGGTTGTGA PRIMER_RIGHT_3_SEQUENCE=AAGAACACCTGTGCTGTGGTC PRIMER_LEFT_3=280,22 PRIMER_RIGHT_3=406,21 PRIMER_LEFT_3_TM=59.633 PRIMER_RIGHT_3_TM=60.213 PRIMER_LEFT_3_GC_PERCENT=45.455 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=10.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=127 PRIMER_PAIR_4_PENALTY=3.589613 PRIMER_LEFT_4_PENALTY=2.366796 PRIMER_RIGHT_4_PENALTY=1.222816 PRIMER_LEFT_4_SEQUENCE=TTTGGACAGACCTAGGTTGTGA PRIMER_RIGHT_4_SEQUENCE=AAAGAACACCTGTGCTGTGG PRIMER_LEFT_4=280,22 PRIMER_RIGHT_4=407,20 PRIMER_LEFT_4_TM=59.633 PRIMER_RIGHT_4_TM=58.777 PRIMER_LEFT_4_GC_PERCENT=45.455 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=10.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=128 = SEQUENCE_ID=39.RHAP64FE12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNACCCCCCANAGCGTCCGTCTCNGNNNNNNNNNNNNNNNNNNNNNNNATGNANNNNNNNNNNNNNNNNNNTANGCCNGNNNNNNNNNNNNNNNNNNNNNNNATATGTCNGTGTGCCNCNNNNNNNNNNNNNNNNNNTATATCNGTGTATATGTGNGTGTACCCNNATGTGTGNGTACNCCTGTCNGTGNATGTGTGTATGNCTCNGCACCNCNCNNNCNCNCTNNATGNCNNNATANNTANCTCNANGTGNNCGCCCCNATGNACATNNCNGTGCCNACANNTCNGNATNCGNCCCNCNATCTCNANCNANGTCNGNNNNNANGNNNGCATATCNNNGTANNCNNANNTCNNTCNATACCNATCTANNNGNNNNNANCNANNNNNCNNNNNATANCCNCNCNCNGTCNCCNATNTANACANNNANNNNTNNGT SEQUENCE_INCLUDED_REGION=23,432 SEQUENCE_TARGET=47,22 SEQUENCE_TARGET=74,18 SEQUENCE_TARGET=101,22 SEQUENCE_TARGET=140,18 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=39.RHAP64FE6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNCCTTTACTTGAATGAAAAGTATTGTTTCTGTCTGTCTCTGTCTCTTTGTCTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGNAGGGNGCGTTACCCACCATAGATTATCCCCGACAATTTCAGTCTTCTCTGAGAATATCATAGTANAGACCCACCAAAATTGTCTCATTTNCAACACTGATCTTGCAAAGAAAATTAACATAAATCCGNNAAACTAATACTGAATANTTATAATATATCCATATCATGTCAACTATTTACATTCATAGAGTCCTCATCTAGAAAAACAATANTCTTTGTTTNTACAGTGGTTTGTACATGGC SEQUENCE_INCLUDED_REGION=16,425 SEQUENCE_TARGET=70,36 SEQUENCE_TARGET=107,54 SEQUENCE_TARGET=162,32 P3_COMMENT=CL is TTCTGTCTGTCTCTGTCTCTTTGTC P3_COMMENT=CR is TCGGGGATAATCTATGGTGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=6.162646 PRIMER_LEFT_0_PENALTY=5.761718 PRIMER_RIGHT_0_PENALTY=0.400928 PRIMER_LEFT_0_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC PRIMER_RIGHT_0_SEQUENCE=TCGGGGATAATCTATGGTGG PRIMER_LEFT_0=41,25 PRIMER_RIGHT_0=231,20 PRIMER_LEFT_0_TM=59.238 PRIMER_RIGHT_0_TM=59.599 PRIMER_LEFT_0_GC_PERCENT=44.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=1.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=191 PRIMER_PAIR_1_PENALTY=6.235412 PRIMER_LEFT_1_PENALTY=5.761718 PRIMER_RIGHT_1_PENALTY=0.473695 PRIMER_LEFT_1_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC PRIMER_RIGHT_1_SEQUENCE=AAGACTGAAATTGTCGGGGA PRIMER_LEFT_1=41,25 PRIMER_RIGHT_1=244,20 PRIMER_LEFT_1_TM=59.238 PRIMER_RIGHT_1_TM=59.526 PRIMER_LEFT_1_GC_PERCENT=44.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=1.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=10.9000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=204 PRIMER_PAIR_2_PENALTY=6.235412 PRIMER_LEFT_2_PENALTY=5.761718 PRIMER_RIGHT_2_PENALTY=0.473695 PRIMER_LEFT_2_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC PRIMER_RIGHT_2_SEQUENCE=GAAGACTGAAATTGTCGGGG PRIMER_LEFT_2=41,25 PRIMER_RIGHT_2=245,20 PRIMER_LEFT_2_TM=59.238 PRIMER_RIGHT_2_TM=59.526 PRIMER_LEFT_2_GC_PERCENT=44.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=1.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=12.9000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=205 PRIMER_PAIR_3_PENALTY=6.473621 PRIMER_LEFT_3_PENALTY=6.072692 PRIMER_RIGHT_3_PENALTY=0.400928 PRIMER_LEFT_3_SEQUENCE=TCTGTCTCTGTCTCTTTGTCTTTG PRIMER_RIGHT_3_SEQUENCE=TCGGGGATAATCTATGGTGG PRIMER_LEFT_3=46,24 PRIMER_RIGHT_3=231,20 PRIMER_LEFT_3_TM=57.927 PRIMER_RIGHT_3_TM=59.599 PRIMER_LEFT_3_GC_PERCENT=41.667 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=1.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=186 PRIMER_PAIR_4_PENALTY=6.473621 PRIMER_LEFT_4_PENALTY=6.072692 PRIMER_RIGHT_4_PENALTY=0.400928 PRIMER_LEFT_4_SEQUENCE=TTTCTGTCTGTCTCTGTCTCTTTG PRIMER_RIGHT_4_SEQUENCE=TCGGGGATAATCTATGGTGG PRIMER_LEFT_4=40,24 PRIMER_RIGHT_4=231,20 PRIMER_LEFT_4_TM=57.927 PRIMER_RIGHT_4_TM=59.599 PRIMER_LEFT_4_GC_PERCENT=41.667 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=1.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=2.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=192 = SEQUENCE_ID=41.RHAP64FF11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCATGATATGGGCGTGGTTTGTGTGCTTGCTAAGTCAGTGAAGCACTCTCTTGAAGGAAGGAGGACCTGAATACAATCCCCCAGAATCTATGATGAGTTGGAAGCAGAACTGTCCATAGTTTTGCAAGACTGTATTTCTTGTGCTGGTATGATGGAGTCACATGATCCATTCAATCATTTGCCAGCCAGACTAACCTCACTAGTTAAGTTTAAGGCTGATAAGGGACCAAATATTAAAAAGAAAGTTGATTTCCAGGAGGAACATCTGAGGTTGTCTTTTAGCCAGTACACATTTGTTTGCAAATATGCACACATACTAGAGAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGAATGGGGGGAGTTCAGAAAATCTCCAGGGATCNGTTNTNA SEQUENCE_INCLUDED_REGION=23,446 SEQUENCE_TARGET=348,74 P3_COMMENT=CL is TCCAGGAGGAACATCTGAGG P3_COMMENT=CR is TTCTGAACTCCCCCCATTC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.343959 PRIMER_LEFT_0_PENALTY=0.190960 PRIMER_RIGHT_0_PENALTY=1.152999 PRIMER_LEFT_0_SEQUENCE=TCCAGGAGGAACATCTGAGG PRIMER_RIGHT_0_SEQUENCE=TTCTGAACTCCCCCCATTC PRIMER_LEFT_0=273,20 PRIMER_RIGHT_0=446,19 PRIMER_LEFT_0_TM=60.191 PRIMER_RIGHT_0_TM=59.847 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=52.632 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 PRIMER_PAIR_1_PENALTY=1.382688 PRIMER_LEFT_1_PENALTY=0.190960 PRIMER_RIGHT_1_PENALTY=1.191728 PRIMER_LEFT_1_SEQUENCE=TCCAGGAGGAACATCTGAGG PRIMER_RIGHT_1_SEQUENCE=TTTCTGAACTCCCCCCATTC PRIMER_LEFT_1=273,20 PRIMER_RIGHT_1=447,20 PRIMER_LEFT_1_TM=60.191 PRIMER_RIGHT_1_TM=61.192 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=175 PRIMER_PAIR_2_PENALTY=1.562074 PRIMER_LEFT_2_PENALTY=0.898913 PRIMER_RIGHT_2_PENALTY=0.663161 PRIMER_LEFT_2_SEQUENCE=CCAGGAGGAACATCTGAGGT PRIMER_RIGHT_2_SEQUENCE=TTTTCTGAACTCCCCCCATT PRIMER_LEFT_2=274,20 PRIMER_RIGHT_2=448,20 PRIMER_LEFT_2_TM=59.101 PRIMER_RIGHT_2_TM=60.663 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.6000 PRIMER_RIGHT_2_END_STABILITY=8.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=175 PRIMER_PAIR_3_PENALTY=1.665234 PRIMER_LEFT_3_PENALTY=1.293870 PRIMER_RIGHT_3_PENALTY=0.371364 PRIMER_LEFT_3_SEQUENCE=CAGGAGGAACATCTGAGGTTG PRIMER_RIGHT_3_SEQUENCE=ATTTTCTGAACTCCCCCCAT PRIMER_LEFT_3=275,21 PRIMER_RIGHT_3=449,20 PRIMER_LEFT_3_TM=59.706 PRIMER_RIGHT_3_TM=59.629 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=9.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=175 PRIMER_PAIR_4_PENALTY=1.724434 PRIMER_LEFT_4_PENALTY=1.353069 PRIMER_RIGHT_4_PENALTY=0.371364 PRIMER_LEFT_4_SEQUENCE=GCCAGTACACATTTGTTTGCA PRIMER_RIGHT_4_SEQUENCE=ATTTTCTGAACTCCCCCCAT PRIMER_LEFT_4=303,21 PRIMER_RIGHT_4=449,20 PRIMER_LEFT_4_TM=59.647 PRIMER_RIGHT_4_TM=59.629 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.8000 PRIMER_RIGHT_4_END_STABILITY=9.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=147 = SEQUENCE_ID=44.RHAP64FG5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNCCTCTTTCCTCTGTAGAAAATGCACTGCTAATAACTTTGCTCAAATATATCTCACAACATTTCTTAATATGGCTCCAGGTAGCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNACAGACAGACAGACAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCAGAAAGAGTGAAACCTAGGTACCCTGGTATTATAAAGAACACATTTTCAGCTCAAAAGCGAGCAGGGNGTCGCTCCACTCTCCATGGGTCTGGGTGTGGCTCCNTCNCCACACTGAAGGGTCTGNTGAGCTCCTGNAGGGGTAACAGGGGCTGCNTCCTCANATTANGGGTTCAGAGGNTGGNGGGTGNGCCACATTTTTTCAAANACA SEQUENCE_INCLUDED_REGION=17,435 SEQUENCE_TARGET=103,40 SEQUENCE_TARGET=144,22 SEQUENCE_TARGET=186,54 P3_COMMENT=CL is TCCTCTGTAGAAAATGCACTGC P3_COMMENT=CR is CAGACCCATGGAGAGTGGAG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.146047 PRIMER_LEFT_0_PENALTY=2.478252 PRIMER_RIGHT_0_PENALTY=0.667795 PRIMER_LEFT_0_SEQUENCE=TCCTCTGTAGAAAATGCACTGC PRIMER_RIGHT_0_SEQUENCE=CAGACCCATGGAGAGTGGAG PRIMER_LEFT_0=23,22 PRIMER_RIGHT_0=334,20 PRIMER_LEFT_0_TM=59.522 PRIMER_RIGHT_0_TM=60.668 PRIMER_LEFT_0_GC_PERCENT=45.455 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=312 PRIMER_PAIR_1_PENALTY=3.488781 PRIMER_LEFT_1_PENALTY=2.478252 PRIMER_RIGHT_1_PENALTY=1.010529 PRIMER_LEFT_1_SEQUENCE=TCCTCTGTAGAAAATGCACTGC PRIMER_RIGHT_1_SEQUENCE=TCGCTTTTGAGCTGAAAATGT PRIMER_LEFT_1=23,22 PRIMER_RIGHT_1=303,21 PRIMER_LEFT_1_TM=59.522 PRIMER_RIGHT_1_TM=60.011 PRIMER_LEFT_1_GC_PERCENT=45.455 PRIMER_RIGHT_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=281 PRIMER_PAIR_2_PENALTY=3.756457 PRIMER_LEFT_2_PENALTY=3.088662 PRIMER_RIGHT_2_PENALTY=0.667795 PRIMER_LEFT_2_SEQUENCE=TGCACTGCTAATAACTTTGCTCA PRIMER_RIGHT_2_SEQUENCE=CAGACCCATGGAGAGTGGAG PRIMER_LEFT_2=37,23 PRIMER_RIGHT_2=334,20 PRIMER_LEFT_2_TM=60.089 PRIMER_RIGHT_2_TM=60.668 PRIMER_LEFT_2_GC_PERCENT=39.130 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=298 PRIMER_PAIR_3_PENALTY=3.789280 PRIMER_LEFT_3_PENALTY=3.121486 PRIMER_RIGHT_3_PENALTY=0.667795 PRIMER_LEFT_3_SEQUENCE=TCTTAATATGGCTCCAGGTAGCA PRIMER_RIGHT_3_SEQUENCE=CAGACCCATGGAGAGTGGAG PRIMER_LEFT_3=78,23 PRIMER_RIGHT_3=334,20 PRIMER_LEFT_3_TM=60.121 PRIMER_RIGHT_3_TM=60.668 PRIMER_LEFT_3_GC_PERCENT=43.478 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.5000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=257 PRIMER_PAIR_4_PENALTY=3.815878 PRIMER_LEFT_4_PENALTY=3.148084 PRIMER_RIGHT_4_PENALTY=0.667795 PRIMER_LEFT_4_SEQUENCE=AATATGGCTCCAGGTAGCAGT PRIMER_RIGHT_4_SEQUENCE=CAGACCCATGGAGAGTGGAG PRIMER_LEFT_4=82,21 PRIMER_RIGHT_4=334,20 PRIMER_LEFT_4_TM=57.852 PRIMER_RIGHT_4_TM=60.668 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=253 = SEQUENCE_ID=45.RHAP64FG12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCTGTCAATATTCCAGAACAGCGCATGCCACCCACACAACGTGCTTCAGCCAGCCCTGCAGTGCCCTTCCTGGACAGGATTTCTTCTTCTTTGAAAGAAAAACAACAACAAAGCTTTTATTATANNTATCNAATTNNNNNNNNNNNNNNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGTATTTGGACATAAAGATGAGACTGGTAGGAAGCTTTGTGTTGGAGACTGTCCTTAAATTCCCTCCATCTTTCCTTCAGGGGAATAGCCCATGCCTGTGGAACCCATCGCTCCTCACTGGGTAGCTGTCAGAAGTGTGAGGATGCTAACAGTCTGTAAACGGTGCTGTCCCTGCAGCGATCCCCAGCAGAGTCTGAGAAACTCCCAATCCACCAGCAAGATGACACAATTGTTTTGCTG SEQUENCE_INCLUDED_REGION=22,442 SEQUENCE_TARGET=156,14 SEQUENCE_TARGET=172,50 P3_COMMENT=CL is TTGAAAGAAAAACAACAACAAAGC P3_COMMENT=CR is CCAACACAAAGCTTCCTACCA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.419108 PRIMER_LEFT_0_PENALTY=4.265519 PRIMER_RIGHT_0_PENALTY=1.153589 PRIMER_LEFT_0_SEQUENCE=TTGAAAGAAAAACAACAACAAAGC PRIMER_RIGHT_0_SEQUENCE=CCAACACAAAGCTTCCTACCA PRIMER_LEFT_0=111,24 PRIMER_RIGHT_0=268,21 PRIMER_LEFT_0_TM=59.734 PRIMER_RIGHT_0_TM=60.154 PRIMER_LEFT_0_GC_PERCENT=29.167 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=7.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=158 PRIMER_PAIR_1_PENALTY=5.505495 PRIMER_LEFT_1_PENALTY=4.265519 PRIMER_RIGHT_1_PENALTY=1.239976 PRIMER_LEFT_1_SEQUENCE=TTGAAAGAAAAACAACAACAAAGC PRIMER_RIGHT_1_SEQUENCE=GGACAGTCTCCAACACAAAGC PRIMER_LEFT_1=111,24 PRIMER_RIGHT_1=277,21 PRIMER_LEFT_1_TM=59.734 PRIMER_RIGHT_1_TM=59.760 PRIMER_LEFT_1_GC_PERCENT=29.167 PRIMER_RIGHT_1_GC_PERCENT=52.381 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=167 PRIMER_PAIR_2_PENALTY=5.570866 PRIMER_LEFT_2_PENALTY=0.821928 PRIMER_RIGHT_2_PENALTY=4.748938 PRIMER_LEFT_2_SEQUENCE=CCCTTCCTGGACAGGATTTC PRIMER_RIGHT_2_SEQUENCE=CCAGTCTCATCTTTATGTCCAAA PRIMER_LEFT_2=84,20 PRIMER_RIGHT_2=250,23 PRIMER_LEFT_2_TM=60.822 PRIMER_RIGHT_2_TM=58.251 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=39.130 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=8.8000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=167 PRIMER_PAIR_3_PENALTY=5.638731 PRIMER_LEFT_3_PENALTY=4.485142 PRIMER_RIGHT_3_PENALTY=1.153589 PRIMER_LEFT_3_SEQUENCE=TGAAAGAAAAACAACAACAAAGC PRIMER_RIGHT_3_SEQUENCE=CCAACACAAAGCTTCCTACCA PRIMER_LEFT_3=112,23 PRIMER_RIGHT_3=268,21 PRIMER_LEFT_3_TM=58.515 PRIMER_RIGHT_3_TM=60.154 PRIMER_LEFT_3_GC_PERCENT=30.435 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=157 PRIMER_PAIR_4_PENALTY=5.725118 PRIMER_LEFT_4_PENALTY=4.485142 PRIMER_RIGHT_4_PENALTY=1.239976 PRIMER_LEFT_4_SEQUENCE=TGAAAGAAAAACAACAACAAAGC PRIMER_RIGHT_4_SEQUENCE=GGACAGTCTCCAACACAAAGC PRIMER_LEFT_4=112,23 PRIMER_RIGHT_4=277,21 PRIMER_LEFT_4_TM=58.515 PRIMER_RIGHT_4_TM=59.760 PRIMER_LEFT_4_GC_PERCENT=30.435 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=166 = SEQUENCE_ID=46.RHAP64FH10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCCAGCCCTGCTAACCACCTAAAGGCACCAGCAGGAGCTNCCCTCAAAAGATCTCACGTTTCCTCTGACAGCACCTTAAAGGAGCACCANTAATGAAGGCTGCTCTGTTGNGTGGGTAGGATGCCAAGGCAGAGGCGGCTTTCTACAGAGCTCCTTCCTCGCCGCAGTAACTCAAGTAGACACCTCAGTCGTTCTCCTGCAGTGTTCTGTGTTCATCACAAGGGGGCAGCCTTGACCCACTTCCCATGGTTGCCAATTCTCCAACCCATCCCCCTCCCCTCATCTAAGAAGACCTTGAACCTGGAGGAGAATATAAATAAACCTGAAAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNATGNGTNTGNNTGGTTAGNGTAAANATGGGGTATGNNNNNNTGGNGNGTTAGANNNANGGTGAGNNTATNTGNATGT SEQUENCE_INCLUDED_REGION=12,456 SEQUENCE_TARGET=343,26 SEQUENCE_TARGET=371,20 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=46.RHAP64FH4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNCCAGCTAGCCTGGCACACACAACAAAACAAGAACCCTTGCTTGAATAAAATGGAAGGTGAGGTCTGATACCCAAAATTAACCTTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGNTATTTTTATAANATAGAGAAAAGNAATTTCATTAAAAGGAANTGAAAGAAGTNTAAATATAATTATANATTTTAGATTAAAANTTTGTAATNAAAGCACACGGTATGTGTTTATTTAAGTGAGAGTACACGTGGAGTTAAAATGTGGATATANATAAAAATNAGNGAGNTTTTAAAAATNANTTTCTTAGNTTGNTTTAGGGATATNTNTNNACCACAAACANATACANAAAACCCAAAANAAANNNNNNNNNNNNNNNNNAGNA SEQUENCE_INCLUDED_REGION=19,433 SEQUENCE_TARGET=107,18 SEQUENCE_TARGET=126,22 SEQUENCE_TARGET=151,32 SEQUENCE_TARGET=433,15 P3_COMMENT=CL is CCTGGCACACACAACAAAAC P3_COMMENT=CR is TCCACATTTTAACTCCACGTG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.508517 PRIMER_LEFT_0_PENALTY=0.048588 PRIMER_RIGHT_0_PENALTY=2.459929 PRIMER_LEFT_0_SEQUENCE=CCTGGCACACACAACAAAAC PRIMER_RIGHT_0_SEQUENCE=TCCACATTTTAACTCCACGTG PRIMER_LEFT_0=27,20 PRIMER_RIGHT_0=334,21 PRIMER_LEFT_0_TM=60.049 PRIMER_RIGHT_0_TM=58.540 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=308 PRIMER_PAIR_1_PENALTY=2.647262 PRIMER_LEFT_1_PENALTY=0.048588 PRIMER_RIGHT_1_PENALTY=2.598673 PRIMER_LEFT_1_SEQUENCE=CCTGGCACACACAACAAAAC PRIMER_RIGHT_1_SEQUENCE=TCCACATTTTAACTCCACGTGT PRIMER_LEFT_1=27,20 PRIMER_RIGHT_1=334,22 PRIMER_LEFT_1_TM=60.049 PRIMER_RIGHT_1_TM=59.401 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=40.909 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=308 PRIMER_PAIR_2_PENALTY=2.851188 PRIMER_LEFT_2_PENALTY=0.391259 PRIMER_RIGHT_2_PENALTY=2.459929 PRIMER_LEFT_2_SEQUENCE=TGGCACACACAACAAAACAA PRIMER_RIGHT_2_SEQUENCE=TCCACATTTTAACTCCACGTG PRIMER_LEFT_2=29,20 PRIMER_RIGHT_2=334,21 PRIMER_LEFT_2_TM=59.609 PRIMER_RIGHT_2_TM=58.540 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=6.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=306 PRIMER_PAIR_3_PENALTY=2.989933 PRIMER_LEFT_3_PENALTY=0.391259 PRIMER_RIGHT_3_PENALTY=2.598673 PRIMER_LEFT_3_SEQUENCE=TGGCACACACAACAAAACAA PRIMER_RIGHT_3_SEQUENCE=TCCACATTTTAACTCCACGTGT PRIMER_LEFT_3=29,20 PRIMER_RIGHT_3=334,22 PRIMER_LEFT_3_TM=59.609 PRIMER_RIGHT_3_TM=59.401 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=40.909 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=306 PRIMER_PAIR_4_PENALTY=3.166398 PRIMER_LEFT_4_PENALTY=0.048588 PRIMER_RIGHT_4_PENALTY=3.117810 PRIMER_LEFT_4_SEQUENCE=CCTGGCACACACAACAAAAC PRIMER_RIGHT_4_SEQUENCE=ATCCACATTTTAACTCCACGTG PRIMER_LEFT_4=27,20 PRIMER_RIGHT_4=335,22 PRIMER_LEFT_4_TM=60.049 PRIMER_RIGHT_4_TM=58.882 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=6.00 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=309 = SEQUENCE_ID=06.RHAP68FB3.seq SEQUENCE_TEMPLATE=NNNNNNNNACACTTGCTTTTGGTAGGTAAAACGCATGGAATTTGATTCCAAACATGACACAAAAGTTCACCTTATTTCCAACTCATTTCATGCACTTGTGTAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAAACCTTTGGGTTTAGCTACATTTAGTTGGTTCTGTTCCTTGCAACTGAAACATTGAGATTTTTCCCACAAGGAAAGAAAGTAAATGGTGGGA SEQUENCE_INCLUDED_REGION=8,243 SEQUENCE_TARGET=104,52 P3_COMMENT=CL is GCATGGAATTTGATTCCAAA P3_COMMENT=CR is TTGCAAGGAACAGAACCAACT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.830638 PRIMER_LEFT_0_PENALTY=1.596296 PRIMER_RIGHT_0_PENALTY=1.234342 PRIMER_LEFT_0_SEQUENCE=GCATGGAATTTGATTCCAAA PRIMER_RIGHT_0_SEQUENCE=TTGCAAGGAACAGAACCAACT PRIMER_LEFT_0=32,20 PRIMER_RIGHT_0=202,21 PRIMER_LEFT_0_TM=58.404 PRIMER_RIGHT_0_TM=59.766 PRIMER_LEFT_0_GC_PERCENT=35.000 PRIMER_RIGHT_0_GC_PERCENT=42.857 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.8000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=171 PRIMER_PAIR_1_PENALTY=2.830638 PRIMER_LEFT_1_PENALTY=1.596296 PRIMER_RIGHT_1_PENALTY=1.234342 PRIMER_LEFT_1_SEQUENCE=GCATGGAATTTGATTCCAAA PRIMER_RIGHT_1_SEQUENCE=AGTTGCAAGGAACAGAACCAA PRIMER_LEFT_1=32,20 PRIMER_RIGHT_1=204,21 PRIMER_LEFT_1_TM=58.404 PRIMER_RIGHT_1_TM=59.766 PRIMER_LEFT_1_GC_PERCENT=35.000 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=9.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=173 PRIMER_PAIR_2_PENALTY=2.847767 PRIMER_LEFT_2_PENALTY=1.596296 PRIMER_RIGHT_2_PENALTY=1.251471 PRIMER_LEFT_2_SEQUENCE=GCATGGAATTTGATTCCAAA PRIMER_RIGHT_2_SEQUENCE=TTGCAAGGAACAGAACCAAC PRIMER_LEFT_2=32,20 PRIMER_RIGHT_2=202,20 PRIMER_LEFT_2_TM=58.404 PRIMER_RIGHT_2_TM=58.749 PRIMER_LEFT_2_GC_PERCENT=35.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=9.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.8000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=171 PRIMER_PAIR_3_PENALTY=2.847767 PRIMER_LEFT_3_PENALTY=1.596296 PRIMER_RIGHT_3_PENALTY=1.251471 PRIMER_LEFT_3_SEQUENCE=GCATGGAATTTGATTCCAAA PRIMER_RIGHT_3_SEQUENCE=GTTGCAAGGAACAGAACCAA PRIMER_LEFT_3=32,20 PRIMER_RIGHT_3=203,20 PRIMER_LEFT_3_TM=58.404 PRIMER_RIGHT_3_TM=58.749 PRIMER_LEFT_3_GC_PERCENT=35.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=9.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.8000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=172 PRIMER_PAIR_4_PENALTY=2.880019 PRIMER_LEFT_4_PENALTY=1.596296 PRIMER_RIGHT_4_PENALTY=1.283723 PRIMER_LEFT_4_SEQUENCE=GCATGGAATTTGATTCCAAA PRIMER_RIGHT_4_SEQUENCE=TCAGTTGCAAGGAACAGAACC PRIMER_LEFT_4=32,20 PRIMER_RIGHT_4=206,21 PRIMER_LEFT_4_TM=58.404 PRIMER_RIGHT_4_TM=60.284 PRIMER_LEFT_4_GC_PERCENT=35.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=9.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.8000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=175 = SEQUENCE_ID=07.RHAP68FC1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNAGGTAGATGATTAGAGAGAACAATCTATTTGGGGTGGATTATTGAGAATGAGAAAACCATTAGAAATGCCAGTAAAACTTCAAATTTGATTACATCTTCATCATCATCATATATTTGTGTGCATGCAAGTGCTCGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCAAAAATCAATAAAATATGCAGTAACTCTTTGTGACTTAGAATTTGGTNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,230 SEQUENCE_TARGET=157,40 P3_COMMENT=CL is TGGGGTGGATTATTGAGAATG P3_COMMENT=CR is TGCATATTTTATTGATTTTTGGCA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.893173 PRIMER_LEFT_0_PENALTY=1.369105 PRIMER_RIGHT_0_PENALTY=4.524067 PRIMER_LEFT_0_SEQUENCE=TGGGGTGGATTATTGAGAATG PRIMER_RIGHT_0_SEQUENCE=TGCATATTTTATTGATTTTTGGCA PRIMER_LEFT_0=48,21 PRIMER_RIGHT_0=221,24 PRIMER_LEFT_0_TM=59.631 PRIMER_RIGHT_0_TM=60.524 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=25.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=10.0000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 PRIMER_PAIR_1_PENALTY=6.215243 PRIMER_LEFT_1_PENALTY=1.369105 PRIMER_RIGHT_1_PENALTY=4.846138 PRIMER_LEFT_1_SEQUENCE=TGGGGTGGATTATTGAGAATG PRIMER_RIGHT_1_SEQUENCE=GCATATTTTATTGATTTTTGGCA PRIMER_LEFT_1=48,21 PRIMER_RIGHT_1=220,23 PRIMER_LEFT_1_TM=59.631 PRIMER_RIGHT_1_TM=58.154 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=26.087 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=173 PRIMER_PAIR_2_PENALTY=6.215243 PRIMER_LEFT_2_PENALTY=1.369105 PRIMER_RIGHT_2_PENALTY=4.846138 PRIMER_LEFT_2_SEQUENCE=TGGGGTGGATTATTGAGAATG PRIMER_RIGHT_2_SEQUENCE=TGCATATTTTATTGATTTTTGGC PRIMER_LEFT_2=48,21 PRIMER_RIGHT_2=221,23 PRIMER_LEFT_2_TM=59.631 PRIMER_RIGHT_2_TM=58.154 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=26.087 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=10.0000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=174 PRIMER_PAIR_3_PENALTY=6.354873 PRIMER_LEFT_3_PENALTY=1.369105 PRIMER_RIGHT_3_PENALTY=4.985768 PRIMER_LEFT_3_SEQUENCE=TGGGGTGGATTATTGAGAATG PRIMER_RIGHT_3_SEQUENCE=CTGCATATTTTATTGATTTTTGGC PRIMER_LEFT_3=48,21 PRIMER_RIGHT_3=222,24 PRIMER_LEFT_3_TM=59.631 PRIMER_RIGHT_3_TM=59.014 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=29.167 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=10.0000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=175 PRIMER_PAIR_4_PENALTY=6.586452 PRIMER_LEFT_4_PENALTY=1.740315 PRIMER_RIGHT_4_PENALTY=4.846138 PRIMER_LEFT_4_SEQUENCE=TTTGGGGTGGATTATTGAGAA PRIMER_RIGHT_4_SEQUENCE=GCATATTTTATTGATTTTTGGCA PRIMER_LEFT_4=46,21 PRIMER_RIGHT_4=220,23 PRIMER_LEFT_4_TM=59.260 PRIMER_RIGHT_4_TM=58.154 PRIMER_LEFT_4_GC_PERCENT=38.095 PRIMER_RIGHT_4_GC_PERCENT=26.087 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=10.0000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=175 = SEQUENCE_ID=08.RHAP68FC2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACATGCATAACCCATTCCTGAATCCATCTGCTGTCAAGCACACAACCCCGTGGCAGAAGCCTAGGGACCAGACAGGAAGCTTCTCCTGAGCTTCCCTGTGGCTGTGGCTAGCAGGAGGCCAGAAGGTCAGCATGGTGGTCCTGTGGGGGAGACACTGCAACCCAGCTAAGCTTCAGCTTGACTCCTGTTNNNNNNNN SEQUENCE_INCLUDED_REGION=0,223 SEQUENCE_TARGET=0,32 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=09.RHAP68FC3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGCATACCTTGTCATTCATGTAGAAGTTTGAAGATAACTTGTGAAGTCAGTTCTACTCTTTTACAATGTGGTTTCTAGGTTTCAAACTTAGGTTTTTAGGCTCAGCAACAAGTCCCTTTACTTCCTAAACCATCATACCAGGACCTGTAAAAGAATTGTCTTTCTTCCTGTTTAATTTAAAAAAACATTATTTTATTTCTGTN SEQUENCE_INCLUDED_REGION=9,241 SEQUENCE_TARGET=9,36 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=11.RHAP68FD2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGTATGCACACACACATGCACCATTATGTTTAACTTTATATATTTGTAAATATTGGTTATATCCTGTGAAGAACGTTTATTGCTAAACACTAAAACATAGAAAAAACAAATGAAGTTTGTTTAGATAATTAAGATGAGAAACTCTTTTGGGTAAGATAGGGTGATGGATTCAGGTAAAATGGGAAGCAGGAGTAATCTGG SEQUENCE_INCLUDED_REGION=0,231 SEQUENCE_TARGET=0,28 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=19.RHAP68FG1.seq SEQUENCE_TEMPLATE=NNNNNNATNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAATAATCTGANTGTGTGTGTGAATCNTCTGATGATAATCTANTGNAGATAGTGATGATGTANTACTGAAGATGTGTNATATANACTGATA SEQUENCE_INCLUDED_REGION=6,257 SEQUENCE_TARGET=10,102 SEQUENCE_TARGET=118,52 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=20.RHAP68FG2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNATCTGGTTCCAATGGATACCCATAATGCAAAATATGCCNNNNNNNNNNNNNCCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGGGGCTCTAGGGGATCTTGCCTTTTAAAGTCAAGAGTTTAGTTGGGGGATTCAGTCTTCACTCACCAGAGGACACCATACAAATAAACTCTTCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=16,208 SEQUENCE_TARGET=54,13 SEQUENCE_TARGET=72,52 P3_COMMENT=CL is TGGTTCCAATGGATACCCATA P3_COMMENT=CR is TGAAGACTGAATCCCCCAAC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.210646 PRIMER_LEFT_0_PENALTY=1.113753 PRIMER_RIGHT_0_PENALTY=0.096893 PRIMER_LEFT_0_SEQUENCE=TGGTTCCAATGGATACCCATA PRIMER_RIGHT_0_SEQUENCE=TGAAGACTGAATCCCCCAAC PRIMER_LEFT_0=19,21 PRIMER_RIGHT_0=187,20 PRIMER_LEFT_0_TM=59.886 PRIMER_RIGHT_0_TM=59.903 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=9.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=169 PRIMER_PAIR_1_PENALTY=1.210646 PRIMER_LEFT_1_PENALTY=1.113753 PRIMER_RIGHT_1_PENALTY=0.096893 PRIMER_LEFT_1_SEQUENCE=TGGTTCCAATGGATACCCATA PRIMER_RIGHT_1_SEQUENCE=GTGAAGACTGAATCCCCCAA PRIMER_LEFT_1=19,21 PRIMER_RIGHT_1=188,20 PRIMER_LEFT_1_TM=59.886 PRIMER_RIGHT_1_TM=59.903 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=9.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.4000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=170 PRIMER_PAIR_2_PENALTY=1.302988 PRIMER_LEFT_2_PENALTY=1.113753 PRIMER_RIGHT_2_PENALTY=0.189235 PRIMER_LEFT_2_SEQUENCE=TGGTTCCAATGGATACCCATA PRIMER_RIGHT_2_SEQUENCE=AAGGCAAGATCCCCTAGAGC PRIMER_LEFT_2=19,21 PRIMER_RIGHT_2=150,20 PRIMER_LEFT_2_TM=59.886 PRIMER_RIGHT_2_TM=59.811 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=9.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.4000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=132 PRIMER_PAIR_3_PENALTY=1.506016 PRIMER_LEFT_3_PENALTY=1.409123 PRIMER_RIGHT_3_PENALTY=0.096893 PRIMER_LEFT_3_SEQUENCE=CCAATGGATACCCATAATGCA PRIMER_RIGHT_3_SEQUENCE=TGAAGACTGAATCCCCCAAC PRIMER_LEFT_3=24,21 PRIMER_RIGHT_3=187,20 PRIMER_LEFT_3_TM=60.409 PRIMER_RIGHT_3_TM=59.903 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.4000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=164 PRIMER_PAIR_4_PENALTY=1.506016 PRIMER_LEFT_4_PENALTY=1.409123 PRIMER_RIGHT_4_PENALTY=0.096893 PRIMER_LEFT_4_SEQUENCE=CCAATGGATACCCATAATGCA PRIMER_RIGHT_4_SEQUENCE=GTGAAGACTGAATCCCCCAA PRIMER_LEFT_4=24,21 PRIMER_RIGHT_4=188,20 PRIMER_LEFT_4_TM=60.409 PRIMER_RIGHT_4_TM=59.903 PRIMER_LEFT_4_GC_PERCENT=42.857 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=10.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=165 = SEQUENCE_ID=25.RHAP68FA4.seq SEQUENCE_TEMPLATE=NNNNNNNNACGAANNNNNNNNNNNNNNNNNNNNGATGATGANNNNNNNNNNNNNNNNNNNNCATGCTCACTTCTGGGGGCTCAGGATGTCATTCCTTAGGCAATGATCATCTATGNGNTTTTGTTTTTGTTTGATAACCATGTATTCTTACCTAGACCTGTATCCCATGAAAGACATCNTGAGAGAGATCTACAAAAATGTCAACATACATATTACAAAACATAGATAGGNACTCATGAGAAATATATAAANCATTGATAGACACCATACAAATAGACTATAGACAANAGNTANAATAGAGAAGNCACAATGCGACACACACNAT SEQUENCE_INCLUDED_REGION=8,317 SEQUENCE_TARGET=13,20 SEQUENCE_TARGET=41,20 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=27.RHAP68FA6.seq SEQUENCE_TEMPLATE=TNCCACTACTATGTCTATGGAGAACACAACAAATTGAAATAGATCAGAGCTAAGGTTCAGATTGTTCTGATCTGGAATTTGGCATCAGTGTTGAAGTTTTGAATGGCTTAGAATACCCAAGTAAATTTTTAACTAAGAAACTGTTTCAGAAGTTCTCCTAAGATCTGNNNNNNNNNNNNNNNNNNNNGATTGCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGATATTTCCT SEQUENCE_INCLUDED_REGION=0,247 SEQUENCE_TARGET=167,20 SEQUENCE_TARGET=195,38 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=28.RHAP68FB4.seq SEQUENCE_TEMPLATE=NNNNNNNACACACACACACATGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTCCTTCACTCAGGCATCTGTTCTTTGTCAATTTTCCTCCTGAAGGGAAAAGACTTAGCACTATTACTCCTTCTGTTTTAGGAGAGATTTTTATTTTTACTT SEQUENCE_INCLUDED_REGION=7,246 SEQUENCE_TARGET=24,72 SEQUENCE_TARGET=104,46 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=32.RHAP68FC5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNTCTTTATNGCCTGTCTAAGACAGAAACAGTAAAGCTGAAACCTAAAGTCAGGGTAGTCAGGCCTAGAAAGGTGGGAAGCAGACAGTTCAGTAATTATCCATGTGCAGGGAACAGAAGAAATACATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATCNCCGCAGNAGNGTGTGTGTGTGGNNNNGTGTGTGGTNATGT SEQUENCE_INCLUDED_REGION=24,244 SEQUENCE_TARGET=156,29 SEQUENCE_TARGET=193,30 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=33.RHAP68FC6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNACGCTTTATTAGTTCCCGAATATGTTCTATTTTCTTTTTGGTATGAGGATTCCTTCAGTTCTCTTCTGTAGTGAAGGTTTACCACTCACACACTCCTACAGCATATTTTTTATAATGGAAAGTTATTTGAAAAACAGATTTATAATTTTTATTTACCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=16,243 SEQUENCE_TARGET=176,46 SEQUENCE_TARGET=223,36 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=35.RHAP68FD5.seq SEQUENCE_TEMPLATE=NNNNNNACGGTGNCCTTGCCTGAAAATAAAAACAGTAAAGATGGAAATGTAGTTTGACAGTCTGCTTGTCCAATGAGCTTATTAGCTTAAGTTTTATCCCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAGATCAGGGAGAAAGATGTTCAGGGAGAGGGAGGGAGAGAGAAAGCAGGAAAAACGGTGTTCTGATACAATGCTCCTCTCCCCNTCTCTTT SEQUENCE_INCLUDED_REGION=6,250 SEQUENCE_TARGET=103,59 P3_COMMENT=CL is TGACAGTCTGCTTGTCCAATG P3_COMMENT=CR is TTCTCTCTCCCTCCCTCTCC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.228790 PRIMER_LEFT_0_PENALTY=1.111304 PRIMER_RIGHT_0_PENALTY=0.117486 PRIMER_LEFT_0_SEQUENCE=TGACAGTCTGCTTGTCCAATG PRIMER_RIGHT_0_SEQUENCE=TTCTCTCTCCCTCCCTCTCC PRIMER_LEFT_0=54,21 PRIMER_RIGHT_0=207,20 PRIMER_LEFT_0_TM=59.889 PRIMER_RIGHT_0_TM=59.883 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=0.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=154 PRIMER_PAIR_1_PENALTY=1.520031 PRIMER_LEFT_1_PENALTY=1.402545 PRIMER_RIGHT_1_PENALTY=0.117486 PRIMER_LEFT_1_SEQUENCE=TCTGCTTGTCCAATGAGCTT PRIMER_RIGHT_1_SEQUENCE=TTCTCTCTCCCTCCCTCTCC PRIMER_LEFT_1=60,20 PRIMER_RIGHT_1=207,20 PRIMER_LEFT_1_TM=58.597 PRIMER_RIGHT_1_TM=59.883 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=0.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=148 PRIMER_PAIR_2_PENALTY=1.579390 PRIMER_LEFT_2_PENALTY=1.111304 PRIMER_RIGHT_2_PENALTY=0.468086 PRIMER_LEFT_2_SEQUENCE=TGACAGTCTGCTTGTCCAATG PRIMER_RIGHT_2_SEQUENCE=TGCTTTCTCTCTCCCTCCCT PRIMER_LEFT_2=54,21 PRIMER_RIGHT_2=211,20 PRIMER_LEFT_2_TM=59.889 PRIMER_RIGHT_2_TM=60.468 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=158 PRIMER_PAIR_3_PENALTY=1.579390 PRIMER_LEFT_3_PENALTY=1.111304 PRIMER_RIGHT_3_PENALTY=0.468086 PRIMER_LEFT_3_SEQUENCE=TGACAGTCTGCTTGTCCAATG PRIMER_RIGHT_3_SEQUENCE=CTGCTTTCTCTCTCCCTCCC PRIMER_LEFT_3=54,21 PRIMER_RIGHT_3=212,20 PRIMER_LEFT_3_TM=59.889 PRIMER_RIGHT_3_TM=60.468 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=159 PRIMER_PAIR_4_PENALTY=1.579390 PRIMER_LEFT_4_PENALTY=1.111304 PRIMER_RIGHT_4_PENALTY=0.468086 PRIMER_LEFT_4_SEQUENCE=TGACAGTCTGCTTGTCCAATG PRIMER_RIGHT_4_SEQUENCE=CCTGCTTTCTCTCTCCCTCC PRIMER_LEFT_4=54,21 PRIMER_RIGHT_4=213,20 PRIMER_LEFT_4_TM=59.889 PRIMER_RIGHT_4_TM=60.468 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=160 = SEQUENCE_ID=36.RHAP68FD6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNACATATACATACATACATACATACATNNNNNNNNNNNNNNATACATATACACATATACATACACACACATACATATACACATATACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAGTTAGGGAAATAAGATTCAAGGAAGAAGCGATATTATTTTCATATCATAAAACTGGAACAGGCTTAAACCAATAGAGAAATTCAAGTGTCTCTGATTCTAAAGTTCAGTCTT SEQUENCE_INCLUDED_REGION=15,249 SEQUENCE_TARGET=41,14 SEQUENCE_TARGET=111,38 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=41.RHAP68FF5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNAATATATGCACACACATATGCGAAACCCATTTTCACTTCTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNTTTGACTACACAGGTATTTGTCAAATTAAGAATGGCAAAATAAAATGTGATGACCTGCATTTGATTAACAGTTAAGGGAACAATCTATTCTTACAGAGGACTCATATTTTTTTCCATGCTCCAATACAGAGTGGAGCATA SEQUENCE_INCLUDED_REGION=18,242 SEQUENCE_TARGET=60,43 SEQUENCE_TARGET=104,16 P3_COMMENT=CL is GCACACACATATGCGAAACC P3_COMMENT=CR is TCAAATGCAGGTCATCACATT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.012201 PRIMER_LEFT_0_PENALTY=0.002648 PRIMER_RIGHT_0_PENALTY=2.009552 PRIMER_LEFT_0_SEQUENCE=GCACACACATATGCGAAACC PRIMER_RIGHT_0_SEQUENCE=TCAAATGCAGGTCATCACATT PRIMER_LEFT_0=25,20 PRIMER_RIGHT_0=183,21 PRIMER_LEFT_0_TM=60.003 PRIMER_RIGHT_0_TM=58.990 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=38.095 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=159 PRIMER_PAIR_1_PENALTY=2.012201 PRIMER_LEFT_1_PENALTY=0.002648 PRIMER_RIGHT_1_PENALTY=2.009552 PRIMER_LEFT_1_SEQUENCE=GCACACACATATGCGAAACC PRIMER_RIGHT_1_SEQUENCE=AATCAAATGCAGGTCATCACA PRIMER_LEFT_1=25,20 PRIMER_RIGHT_1=185,21 PRIMER_LEFT_1_TM=60.003 PRIMER_RIGHT_1_TM=58.990 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=161 PRIMER_PAIR_2_PENALTY=2.135505 PRIMER_LEFT_2_PENALTY=0.002648 PRIMER_RIGHT_2_PENALTY=2.132857 PRIMER_LEFT_2_SEQUENCE=GCACACACATATGCGAAACC PRIMER_RIGHT_2_SEQUENCE=CAAATGCAGGTCATCACATTTT PRIMER_LEFT_2=25,20 PRIMER_RIGHT_2=182,22 PRIMER_LEFT_2_TM=60.003 PRIMER_RIGHT_2_TM=59.867 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=36.364 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=7.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=158 PRIMER_PAIR_3_PENALTY=2.162551 PRIMER_LEFT_3_PENALTY=0.152999 PRIMER_RIGHT_3_PENALTY=2.009552 PRIMER_LEFT_3_SEQUENCE=ACACACATATGCGAAACCCA PRIMER_RIGHT_3_SEQUENCE=TCAAATGCAGGTCATCACATT PRIMER_LEFT_3=27,20 PRIMER_RIGHT_3=183,21 PRIMER_LEFT_3_TM=59.847 PRIMER_RIGHT_3_TM=58.990 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=38.095 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=157 PRIMER_PAIR_4_PENALTY=2.162551 PRIMER_LEFT_4_PENALTY=0.152999 PRIMER_RIGHT_4_PENALTY=2.009552 PRIMER_LEFT_4_SEQUENCE=ACACACATATGCGAAACCCA PRIMER_RIGHT_4_SEQUENCE=AATCAAATGCAGGTCATCACA PRIMER_LEFT_4=27,20 PRIMER_RIGHT_4=185,21 PRIMER_LEFT_4_TM=59.847 PRIMER_RIGHT_4_TM=58.990 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=159 = SEQUENCE_ID=42.RHAP68FF6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNACTCANGATGCTGATCCAATTATCTACAACCTTATAAACATATTTGATAAGTATTCTGAAAAGGCAATCATGAATACACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGTTCCACTGAAAATTTGGAATGAGTTTTTCATTCCTCAAGTTCTTTAGAAATAAAAAATTCTGCTAAGAAAATCATTAGCTCAAAAGACAATGAAAGTAACAAATTATTTAGCTCTTTCAAG SEQUENCE_INCLUDED_REGION=16,245 SEQUENCE_TARGET=98,38 P3_COMMENT=CL is TGCTGATCCAATTATCTACAACC P3_COMMENT=CR is TCCAAATTTTCAGTGGAACATG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=7.010692 PRIMER_LEFT_0_PENALTY=4.849609 PRIMER_RIGHT_0_PENALTY=2.161083 PRIMER_LEFT_0_SEQUENCE=TGCTGATCCAATTATCTACAACC PRIMER_RIGHT_0_SEQUENCE=TCCAAATTTTCAGTGGAACATG PRIMER_LEFT_0=24,23 PRIMER_RIGHT_0=157,22 PRIMER_LEFT_0_TM=58.150 PRIMER_RIGHT_0_TM=59.839 PRIMER_LEFT_0_GC_PERCENT=39.130 PRIMER_RIGHT_0_GC_PERCENT=36.364 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=134 PRIMER_PAIR_1_PENALTY=7.079039 PRIMER_LEFT_1_PENALTY=4.917956 PRIMER_RIGHT_1_PENALTY=2.161083 PRIMER_LEFT_1_SEQUENCE=TGCTGATCCAATTATCTACAACCT PRIMER_RIGHT_1_SEQUENCE=TCCAAATTTTCAGTGGAACATG PRIMER_LEFT_1=24,24 PRIMER_RIGHT_1=157,22 PRIMER_LEFT_1_TM=59.082 PRIMER_RIGHT_1_TM=59.839 PRIMER_LEFT_1_GC_PERCENT=37.500 PRIMER_RIGHT_1_GC_PERCENT=36.364 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=134 PRIMER_PAIR_2_PENALTY=7.275237 PRIMER_LEFT_2_PENALTY=5.114154 PRIMER_RIGHT_2_PENALTY=2.161083 PRIMER_LEFT_2_SEQUENCE=GATGCTGATCCAATTATCTACAACC PRIMER_RIGHT_2_SEQUENCE=TCCAAATTTTCAGTGGAACATG PRIMER_LEFT_2=22,25 PRIMER_RIGHT_2=157,22 PRIMER_LEFT_2_TM=60.114 PRIMER_RIGHT_2_TM=59.839 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=36.364 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=136 PRIMER_PAIR_3_PENALTY=7.418295 PRIMER_LEFT_3_PENALTY=5.257212 PRIMER_RIGHT_3_PENALTY=2.161083 PRIMER_LEFT_3_SEQUENCE=TGCTGATCCAATTATCTACAACCTT PRIMER_RIGHT_3_SEQUENCE=TCCAAATTTTCAGTGGAACATG PRIMER_LEFT_3=24,25 PRIMER_RIGHT_3=157,22 PRIMER_LEFT_3_TM=60.257 PRIMER_RIGHT_3_TM=59.839 PRIMER_LEFT_3_GC_PERCENT=36.000 PRIMER_RIGHT_3_GC_PERCENT=36.364 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=134 PRIMER_PAIR_4_PENALTY=7.420731 PRIMER_LEFT_4_PENALTY=4.849609 PRIMER_RIGHT_4_PENALTY=2.571122 PRIMER_LEFT_4_SEQUENCE=TGCTGATCCAATTATCTACAACC PRIMER_RIGHT_4_SEQUENCE=TCATTCCAAATTTTCAGTGGAA PRIMER_LEFT_4=24,23 PRIMER_RIGHT_4=161,22 PRIMER_LEFT_4_TM=58.150 PRIMER_RIGHT_4_TM=59.429 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=31.818 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=5.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=138 = SEQUENCE_ID=45.RHAP68FG6.seq SEQUENCE_TEMPLATE=NNNNNNNACANNATACANGTTGCTACTAGGTCCTATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATATGTATATATGTTCAANNNNNNNNNNNNNNNNNNNNNNNCTTTTAGCTTATTTTTATGTGACTCCTGGCTATGAAAAGGAGCGGGTGCTGACACTGGTTGTAGCTCTTGTGACACTTTTCCTCCTGTTGGGGTT SEQUENCE_INCLUDED_REGION=7,240 SEQUENCE_TARGET=39,67 SEQUENCE_TARGET=129,23 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=48.RHAP68FH6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNTTATGTATTTAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACAATCATGTGCTGTGTTATAACAACAAAGAGTAAATAAAACGGCCATGAATTTGAGAGAGAGCATGGAGGGTTTATGGGAGGGAGGAAAGGAAAAGCTCATATGATATAATTTTAGAAAGAGAAAAAAAATCAGGAAACACATGTATTCAGTTTTCCCAAAGACTCTGAAGCGTAACACTAGTTTATGCAGCAGAATG SEQUENCE_INCLUDED_REGION=17,246 SEQUENCE_TARGET=31,32 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=01.RHAP69FA7.seq SEQUENCE_TEMPLATE=TGNGNTACGTTNCNCTGNTTTGGCCGTTTTTTGCCNATCCGGNGTCACACTGTTTGAGCCACGGATATCCCAGTCAGCTGTTTCCTACCTTTCCACATAAGGGAGAAATCTTAGTCCTTATGTGGGGCCAAGAATACATGGGGTGGGGGTGGGGGCATAAACAGGTTTTGCTTCTTGCGCTATATGGAATGGAAAGAACCAGACTACAGAGCGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCCTANACAAATTGGGGAGGGGGGTNTAGAAACCCAGGGCAAGCAGATCCCAGGAGCACCGAGNAGGC SEQUENCE_INCLUDED_REGION=0,326 SEQUENCE_TARGET=217,40 P3_COMMENT=CL is AGGTTTTGCTTCTTGCGCTA P3_COMMENT=CR is GTGCTCCTGGGATCTGCTT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.516964 PRIMER_LEFT_0_PENALTY=0.151911 PRIMER_RIGHT_0_PENALTY=1.365052 PRIMER_LEFT_0_SEQUENCE=AGGTTTTGCTTCTTGCGCTA PRIMER_RIGHT_0_SEQUENCE=GTGCTCCTGGGATCTGCTT PRIMER_LEFT_0=162,20 PRIMER_RIGHT_0=316,19 PRIMER_LEFT_0_TM=60.152 PRIMER_RIGHT_0_TM=60.365 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=9.2000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=155 PRIMER_PAIR_1_PENALTY=1.784778 PRIMER_LEFT_1_PENALTY=0.419726 PRIMER_RIGHT_1_PENALTY=1.365052 PRIMER_LEFT_1_SEQUENCE=AACAGGTTTTGCTTCTTGCG PRIMER_RIGHT_1_SEQUENCE=GTGCTCCTGGGATCTGCTT PRIMER_LEFT_1=159,20 PRIMER_RIGHT_1=316,19 PRIMER_LEFT_1_TM=60.420 PRIMER_RIGHT_1_TM=60.365 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=10.5000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=158 PRIMER_PAIR_2_PENALTY=1.798166 PRIMER_LEFT_2_PENALTY=0.433114 PRIMER_RIGHT_2_PENALTY=1.365052 PRIMER_LEFT_2_SEQUENCE=TGCTTCTTGCGCTATATGGA PRIMER_RIGHT_2_SEQUENCE=GTGCTCCTGGGATCTGCTT PRIMER_LEFT_2=168,20 PRIMER_RIGHT_2=316,19 PRIMER_LEFT_2_TM=59.567 PRIMER_RIGHT_2_TM=60.365 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=149 PRIMER_PAIR_3_PENALTY=1.863941 PRIMER_LEFT_3_PENALTY=0.498888 PRIMER_RIGHT_3_PENALTY=1.365052 PRIMER_LEFT_3_SEQUENCE=GGTTTTGCTTCTTGCGCTAT PRIMER_RIGHT_3_SEQUENCE=GTGCTCCTGGGATCTGCTT PRIMER_LEFT_3=163,20 PRIMER_RIGHT_3=316,19 PRIMER_LEFT_3_TM=59.501 PRIMER_RIGHT_3_TM=60.365 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=57.895 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.1000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=2.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=154 PRIMER_PAIR_4_PENALTY=2.239938 PRIMER_LEFT_4_PENALTY=0.874885 PRIMER_RIGHT_4_PENALTY=1.365052 PRIMER_LEFT_4_SEQUENCE=TGCGCTATATGGAATGGAAA PRIMER_RIGHT_4_SEQUENCE=GTGCTCCTGGGATCTGCTT PRIMER_LEFT_4=175,20 PRIMER_RIGHT_4=316,19 PRIMER_LEFT_4_TM=59.125 PRIMER_RIGHT_4_TM=60.365 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=57.895 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=142 = SEQUENCE_ID=03.RHAP69FA9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNACGTTCTCCTGTAGGACAAAGACTTTCTGTGGTTACGACGGATATTTCTCAGACTTTATCATTAAAAAGAACTGACATACAGCTGCGGCTGTAGTTCACATGTAGGATATTTGCTGAGCATGGATGAAACTCTGGGTTTGAGTCCCACCATCAGAAAAATGTGCTTCCCATTTATATGGAGAGTTATTAAATTTGTTCTCACAGTTTGATCCATTTCTCCTCAATATAAAGATCTATGAAGGAATAAGAAGGGAGAGTTCTGCTTTGATGTTAATTAGCAAGAAAGACGACATAGCAAGAAACAGAGCTGAGTCCAGATTCCAGCACATGTGCGCTNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,350 SEQUENCE_TARGET=356,14 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=04.RHAP69FB1.seq SEQUENCE_TEMPLATE=CNGNATTATNTCCANAAANAAATTATTTNATTGCAACANAAAACATTACATGTCAGCCTTAAAAAGCAATTAAATCAAAAAAACAAAAATACCAATTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCATCTCAAGACTCTGTTTCTGTTGTGGGGCCTGTGGGGTCTGGAGCTCCTTTGAAACGGAGACTTGGAAATTGCTCACCTGGCTGAGCTTTGGCCCCTCAGAGGTGGGACTTTCTTTACTAACAATAGGAAGCTGGAGAAGCTGTCAAGTGCGAATGGAGGAGACCTGCTCAGAAAGGGAGGGGAGCTGTGAGAATTTGGTTTCTCTCAGTATTGGGGTGGGGGTGGGGTCTGGATTTACTTGTGCCTAAGATTCCTTTAAAACTCTGACCCGATGCA SEQUENCE_INCLUDED_REGION=0,435 SEQUENCE_TARGET=100,52 P3_COMMENT=CL is CATGTCAGCCTTAAAAAGCAA P3_COMMENT=CR is TCTCCGTTTCAAAGGAGCTC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.339631 PRIMER_LEFT_0_PENALTY=2.889802 PRIMER_RIGHT_0_PENALTY=0.449829 PRIMER_LEFT_0_SEQUENCE=CATGTCAGCCTTAAAAAGCAA PRIMER_RIGHT_0_SEQUENCE=TCTCCGTTTCAAAGGAGCTC PRIMER_LEFT_0=48,21 PRIMER_RIGHT_0=218,20 PRIMER_LEFT_0_TM=58.110 PRIMER_RIGHT_0_TM=59.550 PRIMER_LEFT_0_GC_PERCENT=38.095 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=9.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=171 PRIMER_PAIR_1_PENALTY=3.397323 PRIMER_LEFT_1_PENALTY=2.889802 PRIMER_RIGHT_1_PENALTY=0.507521 PRIMER_LEFT_1_SEQUENCE=CATGTCAGCCTTAAAAAGCAA PRIMER_RIGHT_1_SEQUENCE=CGTTTCAAAGGAGCTCCAGA PRIMER_LEFT_1=48,21 PRIMER_RIGHT_1=214,20 PRIMER_LEFT_1_TM=58.110 PRIMER_RIGHT_1_TM=60.508 PRIMER_LEFT_1_GC_PERCENT=38.095 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=167 PRIMER_PAIR_2_PENALTY=3.484515 PRIMER_LEFT_2_PENALTY=2.889802 PRIMER_RIGHT_2_PENALTY=0.594713 PRIMER_LEFT_2_SEQUENCE=CATGTCAGCCTTAAAAAGCAA PRIMER_RIGHT_2_SEQUENCE=TTTCAAAGGAGCTCCAGACC PRIMER_LEFT_2=48,21 PRIMER_RIGHT_2=212,20 PRIMER_LEFT_2_TM=58.110 PRIMER_RIGHT_2_TM=59.405 PRIMER_LEFT_2_GC_PERCENT=38.095 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=165 PRIMER_PAIR_3_PENALTY=3.504849 PRIMER_LEFT_3_PENALTY=3.055020 PRIMER_RIGHT_3_PENALTY=0.449829 PRIMER_LEFT_3_SEQUENCE=ACATGTCAGCCTTAAAAAGCAA PRIMER_RIGHT_3_SEQUENCE=TCTCCGTTTCAAAGGAGCTC PRIMER_LEFT_3=47,22 PRIMER_RIGHT_3=218,20 PRIMER_LEFT_3_TM=58.945 PRIMER_RIGHT_3_TM=59.550 PRIMER_LEFT_3_GC_PERCENT=36.364 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=9.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=6.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=172 PRIMER_PAIR_4_PENALTY=3.562541 PRIMER_LEFT_4_PENALTY=3.055020 PRIMER_RIGHT_4_PENALTY=0.507521 PRIMER_LEFT_4_SEQUENCE=ACATGTCAGCCTTAAAAAGCAA PRIMER_RIGHT_4_SEQUENCE=CGTTTCAAAGGAGCTCCAGA PRIMER_LEFT_4=47,22 PRIMER_RIGHT_4=214,20 PRIMER_LEFT_4_TM=58.945 PRIMER_RIGHT_4_TM=60.508 PRIMER_LEFT_4_GC_PERCENT=36.364 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=8.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=168 = SEQUENCE_ID=09.RHAP69FC9.seq SEQUENCE_TEMPLATE=NNNNCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGGTTAGTTAAGAAGACAGTTAAAGATGGATTGAAAGTAAGCATTGTGGTTTGTGGTTAGACCTTGTCAAACTGTTATCCAGATAAGTCATAAAATATTTCAATGGTTCGTTTGAACCGATATAAATTCTTTCTAAAGAAATACTATAGTATTGCATTAGCAATATGGCACTGTGATAAATTAGAAAAGAGATTAAGTGTTCACACCTCGGATTGTTGTTTTCTCACTAAGCAATTATGTTAATTCAGAACTATGCAAAGCAAGGAGGAAAGAAAATAAGAAGCCACAAAGA SEQUENCE_INCLUDED_REGION=4,350 SEQUENCE_TARGET=9,52 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=10.RHAP69FD1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNACTTTTTAAAAAACCAAAAATCAGGGGAGATGGCTCACACAGCAAAGGGCCAGATGTGTGAGCTTAAGGTTCGAGGCACTCCTGTCCAGTGTCTTCTGAGATTATTACAGTTCTTGGGAAGTAAAGACATGAAGATGACTGGACCAAACTTGTCAGCCCACTCTAGCAAAATAAGACTGCTAGGTTCAATGAAAAACCCTATCTAAAAAAATAAGGAAAGTGATGAAGAGTGAAGACACCTGATGTTAGCTTTTGACCTCCAAGTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCATACATACATGCACACACATGTGCATGCACATACAAACAATCATGCACACAGACATAAGCTCTAGAAATTACCATCAGTAATGCAACAGACTTTTTAAAGACTGGTATTCTAATAGTAAGTNNNNNNNNN SEQUENCE_INCLUDED_REGION=16,443 SEQUENCE_TARGET=284,50 P3_COMMENT=CL is TGCTAGGTTCAATGAAAAACCC P3_COMMENT=CR is ATGCACATGTGTGTGCATGT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.901866 PRIMER_LEFT_0_PENALTY=2.343977 PRIMER_RIGHT_0_PENALTY=0.557889 PRIMER_LEFT_0_SEQUENCE=TGCTAGGTTCAATGAAAAACCC PRIMER_RIGHT_0_SEQUENCE=ATGCACATGTGTGTGCATGT PRIMER_LEFT_0=193,22 PRIMER_RIGHT_0=363,20 PRIMER_LEFT_0_TM=60.344 PRIMER_RIGHT_0_TM=59.442 PRIMER_LEFT_0_GC_PERCENT=40.909 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=10.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=171 PRIMER_PAIR_1_PENALTY=3.230093 PRIMER_LEFT_1_PENALTY=2.343977 PRIMER_RIGHT_1_PENALTY=0.886116 PRIMER_LEFT_1_SEQUENCE=TGCTAGGTTCAATGAAAAACCC PRIMER_RIGHT_1_SEQUENCE=TGCACATGTGTGTGCATGTA PRIMER_LEFT_1=193,22 PRIMER_RIGHT_1=362,20 PRIMER_LEFT_1_TM=60.344 PRIMER_RIGHT_1_TM=59.114 PRIMER_LEFT_1_GC_PERCENT=40.909 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=5.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=170 PRIMER_PAIR_2_PENALTY=3.658858 PRIMER_LEFT_2_PENALTY=3.100969 PRIMER_RIGHT_2_PENALTY=0.557889 PRIMER_LEFT_2_SEQUENCE=TGATGAAGAGTGAAGACACCTGA PRIMER_RIGHT_2_SEQUENCE=ATGCACATGTGTGTGCATGT PRIMER_LEFT_2=236,23 PRIMER_RIGHT_2=363,20 PRIMER_LEFT_2_TM=59.899 PRIMER_RIGHT_2_TM=59.442 PRIMER_LEFT_2_GC_PERCENT=43.478 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=10.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=128 PRIMER_PAIR_3_PENALTY=3.823442 PRIMER_LEFT_3_PENALTY=3.265553 PRIMER_RIGHT_3_PENALTY=0.557889 PRIMER_LEFT_3_SEQUENCE=GCTAGGTTCAATGAAAAACCC PRIMER_RIGHT_3_SEQUENCE=ATGCACATGTGTGTGCATGT PRIMER_LEFT_3=194,21 PRIMER_RIGHT_3=363,20 PRIMER_LEFT_3_TM=57.734 PRIMER_RIGHT_3_TM=59.442 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=7.00 PRIMER_RIGHT_3_SELF_ANY=10.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=170 PRIMER_PAIR_4_PENALTY=3.863755 PRIMER_LEFT_4_PENALTY=3.305865 PRIMER_RIGHT_4_PENALTY=0.557889 PRIMER_LEFT_4_SEQUENCE=GCTAGGTTCAATGAAAAACCCT PRIMER_RIGHT_4_SEQUENCE=ATGCACATGTGTGTGCATGT PRIMER_LEFT_4=194,22 PRIMER_RIGHT_4=363,20 PRIMER_LEFT_4_TM=58.694 PRIMER_RIGHT_4_TM=59.442 PRIMER_LEFT_4_GC_PERCENT=40.909 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=10.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=9.1000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=170 = SEQUENCE_ID=13.RHAP69FE1.seq SEQUENCE_TEMPLATE=AAACAAACATAAAGTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCAGAAAAATGTTCCTGCAGTGATGTGGATGAAGGATAGGGATGGATTGAAGACGCCAGCAGGCTGGCTTTGTGAGAGTCCAAATAAAACCCTGACAGCAGAGGCATGAGAAGGATTCCAGAAATTTTTAGAACATCACAGCCCAGGCTTTGGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,233 SEQUENCE_TARGET=20,56 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=15.RHAP69FE3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNACACAATTCATTTAAACCACATGCTAGAGTCTACCCTTACAAAAAAAGCAAAAAAGCAAAATATTATAACTTAATTTTTTGATTTCTCTCTTCTATATCATGTCCTACTCTCTCCTTTGTAGATACCATCTCTACCACCTGGTAACACAGCATTTCTTTTTTTCTCTTTCTACATGAATATATCTAGACGACTCAGACACTCATCTACATAAAGAGAAAAATAAGCCAAGACCCTCCCTCCCTCATATGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTTCCATGAGTGTGAAAATGTCCCTGTGGGGAAAATAGCCAGTAGTAGCCAGTATGCAAATTTAAAAAGAAAAGGTTCAGTTCTTCATTCTTCAATTCCTGTCTCACATCATCTTTGTTGCCTTAAGGACAATGAAAGAATCAAAATATCTGCTAGAAACTTTCCTTGGT SEQUENCE_INCLUDED_REGION=17,446 SEQUENCE_TARGET=272,22 P3_COMMENT=CL is CCACCTGGTAACACAGCATTT P3_COMMENT=CR is CCACAGGGACATTTTCACACT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.218449 PRIMER_LEFT_0_PENALTY=1.094209 PRIMER_RIGHT_0_PENALTY=1.124240 PRIMER_LEFT_0_SEQUENCE=CCACCTGGTAACACAGCATTT PRIMER_RIGHT_0_SEQUENCE=CCACAGGGACATTTTCACACT PRIMER_LEFT_0=151,21 PRIMER_RIGHT_0=321,21 PRIMER_LEFT_0_TM=59.906 PRIMER_RIGHT_0_TM=59.876 PRIMER_LEFT_0_GC_PERCENT=47.619 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.2000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=171 PRIMER_PAIR_1_PENALTY=2.251449 PRIMER_LEFT_1_PENALTY=1.094209 PRIMER_RIGHT_1_PENALTY=1.157240 PRIMER_LEFT_1_SEQUENCE=CCACCTGGTAACACAGCATTT PRIMER_RIGHT_1_SEQUENCE=TTCCCCACAGGGACATTTT PRIMER_LEFT_1=151,21 PRIMER_RIGHT_1=325,19 PRIMER_LEFT_1_TM=59.906 PRIMER_RIGHT_1_TM=60.157 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=47.368 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.2000 PRIMER_RIGHT_1_END_STABILITY=7.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=175 PRIMER_PAIR_2_PENALTY=2.267158 PRIMER_LEFT_2_PENALTY=1.094209 PRIMER_RIGHT_2_PENALTY=1.172949 PRIMER_LEFT_2_SEQUENCE=CCACCTGGTAACACAGCATTT PRIMER_RIGHT_2_SEQUENCE=CCACAGGGACATTTTCACAC PRIMER_LEFT_2=151,21 PRIMER_RIGHT_2=321,20 PRIMER_LEFT_2_TM=59.906 PRIMER_RIGHT_2_TM=58.827 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.2000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=171 PRIMER_PAIR_3_PENALTY=2.319369 PRIMER_LEFT_3_PENALTY=1.094209 PRIMER_RIGHT_3_PENALTY=1.225160 PRIMER_LEFT_3_SEQUENCE=CCACCTGGTAACACAGCATTT PRIMER_RIGHT_3_SEQUENCE=GGGACATTTTCACACTCATGG PRIMER_LEFT_3=151,21 PRIMER_RIGHT_3=316,21 PRIMER_LEFT_3_TM=59.906 PRIMER_RIGHT_3_TM=60.225 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.2000 PRIMER_RIGHT_3_END_STABILITY=8.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=166 PRIMER_PAIR_4_PENALTY=2.369803 PRIMER_LEFT_4_PENALTY=0.110099 PRIMER_RIGHT_4_PENALTY=2.259704 PRIMER_LEFT_4_SEQUENCE=AAGACCCTCCCTCCCTCATA PRIMER_RIGHT_4_SEQUENCE=AGGCAACAAAGATGATGTGAGA PRIMER_LEFT_4=244,20 PRIMER_RIGHT_4=415,22 PRIMER_LEFT_4_TM=59.890 PRIMER_RIGHT_4_TM=59.740 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=5.9000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=172 = SEQUENCE_ID=21.RHAP69FG3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCAGCCATTCACAGTGCCTTCTCTGTCTCCTGCAGTCAGTTCAGGATGTAAGCCCTCAGCTACTGCAATGATCCCCAAATGAGAAATGAAATGTCTTAGTGAACTTTTTTTTTCAAAAAGTTAGGTATGCTTAGTGGTAGGAAGATGGCTCATTTGATAAAATGCTTGCCCCACACTCAAGCGTGATGATCTGTGTTTGGTTCTCTTATACCTATGATTAATAACAAAACCAAGAGTGGGGAGATCCTGCCTATTATCCCAACACTAGGAAGGCACAAATAGGAAGATCCCTGAAGCCTACCAACCTACCGTAGTCATTGAGCTCCAGGTTCAGGGAGAGACTCAAAAAATAAGATAGAGAATTGTAGAGAAAAATGTAAGCCCTCAACCTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAG SEQUENCE_INCLUDED_REGION=31,446 SEQUENCE_TARGET=427,48 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=25.RHAP69FA4.seq SEQUENCE_TEMPLATE=NNNNNNCCTATAATGTAGGCACTGTTCTGTTTTACAAGTGACACAATTATTTCCTTGGCAGGCACAGAGCCTGAGTGTCCCCTCCAATCCCCCACACCAGGGCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCATCTCCTACAAGGTGTGTGTGAACGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=6,175 SEQUENCE_TARGET=106,44 P3_COMMENT=CL is AATTATTTCCTTGGCAGGCA P3_COMMENT=CR is CCACGTTCACACACACCTTG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.554604 PRIMER_LEFT_0_PENALTY=0.453004 PRIMER_RIGHT_0_PENALTY=1.101601 PRIMER_LEFT_0_SEQUENCE=AATTATTTCCTTGGCAGGCA PRIMER_RIGHT_0_SEQUENCE=CCACGTTCACACACACCTTG PRIMER_LEFT_0=44,20 PRIMER_RIGHT_0=180,20 PRIMER_LEFT_0_TM=59.547 PRIMER_RIGHT_0_TM=61.102 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.7000 PRIMER_RIGHT_0_END_STABILITY=8.5000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=137 PRIMER_PAIR_1_PENALTY=1.554604 PRIMER_LEFT_1_PENALTY=0.453004 PRIMER_RIGHT_1_PENALTY=1.101601 PRIMER_LEFT_1_SEQUENCE=CAATTATTTCCTTGGCAGGC PRIMER_RIGHT_1_SEQUENCE=CCACGTTCACACACACCTTG PRIMER_LEFT_1=43,20 PRIMER_RIGHT_1=180,20 PRIMER_LEFT_1_TM=59.547 PRIMER_RIGHT_1_TM=61.102 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=138 PRIMER_PAIR_2_PENALTY=2.055158 PRIMER_LEFT_2_PENALTY=0.953558 PRIMER_RIGHT_2_PENALTY=1.101601 PRIMER_LEFT_2_SEQUENCE=ATTATTTCCTTGGCAGGCAC PRIMER_RIGHT_2_SEQUENCE=CCACGTTCACACACACCTTG PRIMER_LEFT_2=45,20 PRIMER_RIGHT_2=180,20 PRIMER_LEFT_2_TM=59.046 PRIMER_RIGHT_2_TM=61.102 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=136 PRIMER_PAIR_3_PENALTY=2.258495 PRIMER_LEFT_3_PENALTY=1.156894 PRIMER_RIGHT_3_PENALTY=1.101601 PRIMER_LEFT_3_SEQUENCE=TATTTCCTTGGCAGGCACAG PRIMER_RIGHT_3_SEQUENCE=CCACGTTCACACACACCTTG PRIMER_LEFT_3=47,20 PRIMER_RIGHT_3=180,20 PRIMER_LEFT_3_TM=61.157 PRIMER_RIGHT_3_TM=61.102 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=134 PRIMER_PAIR_4_PENALTY=2.305889 PRIMER_LEFT_4_PENALTY=1.204289 PRIMER_RIGHT_4_PENALTY=1.101601 PRIMER_LEFT_4_SEQUENCE=TATTTCCTTGGCAGGCACA PRIMER_RIGHT_4_SEQUENCE=CCACGTTCACACACACCTTG PRIMER_LEFT_4=47,19 PRIMER_RIGHT_4=180,20 PRIMER_LEFT_4_TM=60.204 PRIMER_RIGHT_4_TM=61.102 PRIMER_LEFT_4_GC_PERCENT=47.368 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=134 = SEQUENCE_ID=26.RHAP69FA11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNACTTATTTACAGAGACTTTTAAGGGTGACAGAAGCCTCTGAGTTAAGCCAGTGGGCACTATGTGATTTTCACATCTCCCAGCAGCTCCAGCATTGCTGTGAACTAGGGAGTTGACCAGTCTTCAAGTGATGCTGCTCTCAGAAACATTAGTCAAGAGGGTAGTTTTAAATTGCTGTTTGATTGGTTNNNNNNNNNNNNNNNNNNNTNCCGNCNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTAAATTAGCTAATCTAGAAACTCACACCTTGTTAAAGTGCAGAGACTAAGTGACCAAGAAGAATGAGTCACAAAGGGGACATCC SEQUENCE_INCLUDED_REGION=18,353 SEQUENCE_TARGET=204,18 SEQUENCE_TARGET=231,18 SEQUENCE_TARGET=250,34 P3_COMMENT=CL is TTTTAAATTGCTGTTTGATTGGTT P3_COMMENT=CR is TCTGCACTTTAACAAGGTGTGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=8.477001 PRIMER_LEFT_0_PENALTY=4.960837 PRIMER_RIGHT_0_PENALTY=3.516163 PRIMER_LEFT_0_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT PRIMER_RIGHT_0_SEQUENCE=TCTGCACTTTAACAAGGTGTGA PRIMER_LEFT_0=180,24 PRIMER_RIGHT_0=329,22 PRIMER_LEFT_0_TM=59.039 PRIMER_RIGHT_0_TM=58.484 PRIMER_LEFT_0_GC_PERCENT=25.000 PRIMER_RIGHT_0_GC_PERCENT=40.909 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=7.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=150 PRIMER_PAIR_1_PENALTY=8.486325 PRIMER_LEFT_1_PENALTY=4.960837 PRIMER_RIGHT_1_PENALTY=3.525488 PRIMER_LEFT_1_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT PRIMER_RIGHT_1_SEQUENCE=TCTGCACTTTAACAAGGTGTGAG PRIMER_LEFT_1=180,24 PRIMER_RIGHT_1=329,23 PRIMER_LEFT_1_TM=59.039 PRIMER_RIGHT_1_TM=59.475 PRIMER_LEFT_1_GC_PERCENT=25.000 PRIMER_RIGHT_1_GC_PERCENT=43.478 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=150 PRIMER_PAIR_2_PENALTY=8.486325 PRIMER_LEFT_2_PENALTY=4.960837 PRIMER_RIGHT_2_PENALTY=3.525488 PRIMER_LEFT_2_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT PRIMER_RIGHT_2_SEQUENCE=CTCTGCACTTTAACAAGGTGTGA PRIMER_LEFT_2=180,24 PRIMER_RIGHT_2=330,23 PRIMER_LEFT_2_TM=59.039 PRIMER_RIGHT_2_TM=59.475 PRIMER_LEFT_2_GC_PERCENT=25.000 PRIMER_RIGHT_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=151 PRIMER_PAIR_3_PENALTY=8.486325 PRIMER_LEFT_3_PENALTY=4.960837 PRIMER_RIGHT_3_PENALTY=3.525488 PRIMER_LEFT_3_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT PRIMER_RIGHT_3_SEQUENCE=TCTCTGCACTTTAACAAGGTGTG PRIMER_LEFT_3=180,24 PRIMER_RIGHT_3=331,23 PRIMER_LEFT_3_TM=59.039 PRIMER_RIGHT_3_TM=59.475 PRIMER_LEFT_3_GC_PERCENT=25.000 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=152 PRIMER_PAIR_4_PENALTY=8.581082 PRIMER_LEFT_4_PENALTY=5.064919 PRIMER_RIGHT_4_PENALTY=3.516163 PRIMER_LEFT_4_SEQUENCE=TCAAGAGGGTAGTTTTAAATTGCTG PRIMER_RIGHT_4_SEQUENCE=TCTGCACTTTAACAAGGTGTGA PRIMER_LEFT_4=168,25 PRIMER_RIGHT_4=329,22 PRIMER_LEFT_4_TM=60.065 PRIMER_RIGHT_4_TM=58.484 PRIMER_LEFT_4_GC_PERCENT=36.000 PRIMER_RIGHT_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=162 = SEQUENCE_ID=27.RHAP69FA6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTATACTTCCTTTCAATTTTTAGCACTTCNAGTCATACATAGGACATAGTCACACATTTCCATTATGCTATAAAATATTGTCAAAATTTAAATAAGTCATCATGGTTGAAGTTATTTCATTTGCTATCTCTAAGATTAGAGCATGCTCTAATATCTTCCTGGTTCCAGAAAATTCAGACCTGTTTTTATCTTATTAAATACCCTCAAGCTCAGCTCATTCTTGAACTGTTGAACTGCAGCCAAGGTGAAGACATTCACATACAAATTAAACATTTTATTTTGCTAATGTTTTTGTGACTGCTTACTACTCAGCAGTAGCTCCTCAAACCAAAAACATTTGATGAGCCATGACTTTGTCATAAATAAGAAAAC SEQUENCE_INCLUDED_REGION=19,447 SEQUENCE_TARGET=23,69 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=31.RHAP69FC10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNACTTAATNTGTTTTTATGATATATTGAATCTCGGTCTCATAATGAGACAGAACATGTGACATTTATTTTCTTCCCCAGTTATTCTATCTTGTTCCCAGTTCTCTTCCTTTGAACAATCAGTCTCCCTTTACAATCTTTTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCAGAATACACCNAGAAATTTCAGTCCGGTGNGCGGTTGATTTCTGGGTATGTCCATATATGTANGTATGTGTATGTGAGTGTCTAGAGAGTTCACAAGAATGCATAGGCTCTTGGGGCTGAAGCTACAGGAAGTTCTCACTTCTCCAATTTGAGTGCTAAGGCATGGA SEQUENCE_INCLUDED_REGION=18,354 SEQUENCE_TARGET=160,42 P3_COMMENT=CL is TCCCAGTTCTCTTCCTTTGAA P3_COMMENT=CR is CATACCCAGAAATCAACCGC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.435943 PRIMER_LEFT_0_PENALTY=2.105078 PRIMER_RIGHT_0_PENALTY=0.330865 PRIMER_LEFT_0_SEQUENCE=TCCCAGTTCTCTTCCTTTGAA PRIMER_RIGHT_0_SEQUENCE=CATACCCAGAAATCAACCGC PRIMER_LEFT_0=110,21 PRIMER_RIGHT_0=254,20 PRIMER_LEFT_0_TM=58.895 PRIMER_RIGHT_0_TM=60.331 PRIMER_LEFT_0_GC_PERCENT=42.857 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=11.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=145 PRIMER_PAIR_1_PENALTY=2.435943 PRIMER_LEFT_1_PENALTY=2.105078 PRIMER_RIGHT_1_PENALTY=0.330865 PRIMER_LEFT_1_SEQUENCE=TTCCCAGTTCTCTTCCTTTGA PRIMER_RIGHT_1_SEQUENCE=CATACCCAGAAATCAACCGC PRIMER_LEFT_1=109,21 PRIMER_RIGHT_1=254,20 PRIMER_LEFT_1_TM=58.895 PRIMER_RIGHT_1_TM=60.331 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=11.1000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=146 PRIMER_PAIR_2_PENALTY=2.544440 PRIMER_LEFT_2_PENALTY=2.213575 PRIMER_RIGHT_2_PENALTY=0.330865 PRIMER_LEFT_2_SEQUENCE=TTCCCAGTTCTCTTCCTTTGAA PRIMER_RIGHT_2_SEQUENCE=CATACCCAGAAATCAACCGC PRIMER_LEFT_2=109,22 PRIMER_RIGHT_2=254,20 PRIMER_LEFT_2_TM=60.214 PRIMER_RIGHT_2_TM=60.331 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=11.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=146 PRIMER_PAIR_3_PENALTY=2.601639 PRIMER_LEFT_3_PENALTY=2.270774 PRIMER_RIGHT_3_PENALTY=0.330865 PRIMER_LEFT_3_SEQUENCE=TCCCAGTTCTCTTCCTTTGAAC PRIMER_RIGHT_3_SEQUENCE=CATACCCAGAAATCAACCGC PRIMER_LEFT_3=110,22 PRIMER_RIGHT_3=254,20 PRIMER_LEFT_3_TM=59.729 PRIMER_RIGHT_3_TM=60.331 PRIMER_LEFT_3_GC_PERCENT=45.455 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=11.1000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=145 PRIMER_PAIR_4_PENALTY=2.601639 PRIMER_LEFT_4_PENALTY=2.270774 PRIMER_RIGHT_4_PENALTY=0.330865 PRIMER_LEFT_4_SEQUENCE=GTTCCCAGTTCTCTTCCTTTGA PRIMER_RIGHT_4_SEQUENCE=CATACCCAGAAATCAACCGC PRIMER_LEFT_4=108,22 PRIMER_RIGHT_4=254,20 PRIMER_LEFT_4_TM=59.729 PRIMER_RIGHT_4_TM=60.331 PRIMER_LEFT_4_GC_PERCENT=45.455 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=11.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=147 = SEQUENCE_ID=32.RHAP69FC11.seq SEQUENCE_TEMPLATE=ANANTAAAGTCCCANCNNNTGTGGGNNGGAGGGTATCCTGGTTACATCAAACCTCTGCTGACTGAGGGCAATAGGGGCAGTATCAGAGAGGCCAAGAAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGGGGGTGGAGGGTGCAGGATGATCCATGAGGGGAATCAGTTGTTGCTTCTGGAAAGATTGGGGTAACAGTCCAGAATATTCATATAGGCAGCAGCCCTGGTGGTTAGTGATGCTGGGATTTAAATCAGGGCTTCAACAGTGTTCGAAAATGTGAGGAAGGCGTCTGTTTCTCACCTCTGATCCTTATTATCAAGTCAGGAGAACTTT SEQUENCE_INCLUDED_REGION=0,362 SEQUENCE_TARGET=101,52 P3_COMMENT=CL is AGGGCAATAGGGGCAGTATC P3_COMMENT=CR is TGATTCCCCTCATGGATCAT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.402924 PRIMER_LEFT_0_PENALTY=0.306903 PRIMER_RIGHT_0_PENALTY=0.096021 PRIMER_LEFT_0_SEQUENCE=AGGGCAATAGGGGCAGTATC PRIMER_RIGHT_0_SEQUENCE=TGATTCCCCTCATGGATCAT PRIMER_LEFT_0=64,20 PRIMER_RIGHT_0=192,20 PRIMER_LEFT_0_TM=60.307 PRIMER_RIGHT_0_TM=60.096 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=5.3000 PRIMER_RIGHT_0_END_STABILITY=6.5000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=129 PRIMER_PAIR_1_PENALTY=0.402924 PRIMER_LEFT_1_PENALTY=0.306903 PRIMER_RIGHT_1_PENALTY=0.096021 PRIMER_LEFT_1_SEQUENCE=GAGGGCAATAGGGGCAGTAT PRIMER_RIGHT_1_SEQUENCE=TGATTCCCCTCATGGATCAT PRIMER_LEFT_1=63,20 PRIMER_RIGHT_1=192,20 PRIMER_LEFT_1_TM=60.307 PRIMER_RIGHT_1_TM=60.096 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=5.3000 PRIMER_RIGHT_1_END_STABILITY=6.5000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=130 PRIMER_PAIR_2_PENALTY=0.490847 PRIMER_LEFT_2_PENALTY=0.306903 PRIMER_RIGHT_2_PENALTY=0.183944 PRIMER_LEFT_2_SEQUENCE=AGGGCAATAGGGGCAGTATC PRIMER_RIGHT_2_SEQUENCE=CCCAATCTTTCCAGAAGCAA PRIMER_LEFT_2=64,20 PRIMER_RIGHT_2=216,20 PRIMER_LEFT_2_TM=60.307 PRIMER_RIGHT_2_TM=60.184 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=5.3000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=153 PRIMER_PAIR_3_PENALTY=0.490847 PRIMER_LEFT_3_PENALTY=0.306903 PRIMER_RIGHT_3_PENALTY=0.183944 PRIMER_LEFT_3_SEQUENCE=GAGGGCAATAGGGGCAGTAT PRIMER_RIGHT_3_SEQUENCE=CCCAATCTTTCCAGAAGCAA PRIMER_LEFT_3=63,20 PRIMER_RIGHT_3=216,20 PRIMER_LEFT_3_TM=60.307 PRIMER_RIGHT_3_TM=60.184 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=5.3000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=154 PRIMER_PAIR_4_PENALTY=0.527748 PRIMER_LEFT_4_PENALTY=0.306903 PRIMER_RIGHT_4_PENALTY=0.220845 PRIMER_LEFT_4_SEQUENCE=AGGGCAATAGGGGCAGTATC PRIMER_RIGHT_4_SEQUENCE=ACAACTGATTCCCCTCATGG PRIMER_LEFT_4=64,20 PRIMER_RIGHT_4=197,20 PRIMER_LEFT_4_TM=60.307 PRIMER_RIGHT_4_TM=59.779 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=5.3000 PRIMER_RIGHT_4_END_STABILITY=8.4000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=134 = SEQUENCE_ID=33.RHAP69FC12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNGCCTCCCAAGTCCCCTCAGCCTCCTCACCACCTTTTATCAGATGGAGTGGAGGAGATGGCAGTCTCTCCCTCCCAGAAGGCTTTGCAGGCTGCTGAAGCCCTTTCCTTCAGGCTGAGCAGCAGCAGCAGCAGCCCCCAACTGGGGCTGTATTATCATTCCATCTTGACCTCAAAGGAAAGAAAGTGAAGCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCACTCACATACTCACACGCGCGCGCNNNNNNNNNNNNNNNNCCCTGTCACCCCAGGCCTCTTATCTTGCTGAATCTGCACCATCTCTCATGAAAGAGGCATGCTT SEQUENCE_INCLUDED_REGION=19,357 SEQUENCE_TARGET=212,54 SEQUENCE_TARGET=296,16 P3_COMMENT=CL is CTTTCCTTCAGGCTGAGCAG P3_COMMENT=CR is GCGCGTGTGAGTATGTGAGT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.332354 PRIMER_LEFT_0_PENALTY=0.268570 PRIMER_RIGHT_0_PENALTY=0.063784 PRIMER_LEFT_0_SEQUENCE=CTTTCCTTCAGGCTGAGCAG PRIMER_RIGHT_0_SEQUENCE=GCGCGTGTGAGTATGTGAGT PRIMER_LEFT_0=119,20 PRIMER_RIGHT_0=291,20 PRIMER_LEFT_0_TM=60.269 PRIMER_RIGHT_0_TM=59.936 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=173 PRIMER_PAIR_1_PENALTY=1.135890 PRIMER_LEFT_1_PENALTY=1.072106 PRIMER_RIGHT_1_PENALTY=0.063784 PRIMER_LEFT_1_SEQUENCE=CCCTTTCCTTCAGGCTGAG PRIMER_RIGHT_1_SEQUENCE=GCGCGTGTGAGTATGTGAGT PRIMER_LEFT_1=117,19 PRIMER_RIGHT_1=291,20 PRIMER_LEFT_1_TM=59.928 PRIMER_RIGHT_1_TM=59.936 PRIMER_LEFT_1_GC_PERCENT=57.895 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.7000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=175 PRIMER_PAIR_2_PENALTY=1.144433 PRIMER_LEFT_2_PENALTY=1.080649 PRIMER_RIGHT_2_PENALTY=0.063784 PRIMER_LEFT_2_SEQUENCE=CCTTTCCTTCAGGCTGAGC PRIMER_RIGHT_2_SEQUENCE=GCGCGTGTGAGTATGTGAGT PRIMER_LEFT_2=118,19 PRIMER_RIGHT_2=291,20 PRIMER_LEFT_2_TM=60.081 PRIMER_RIGHT_2_TM=59.936 PRIMER_LEFT_2_GC_PERCENT=57.895 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=174 PRIMER_PAIR_3_PENALTY=1.223918 PRIMER_LEFT_3_PENALTY=1.160134 PRIMER_RIGHT_3_PENALTY=0.063784 PRIMER_LEFT_3_SEQUENCE=TGGGGCTGTATTATCATTCCA PRIMER_RIGHT_3_SEQUENCE=GCGCGTGTGAGTATGTGAGT PRIMER_LEFT_3=159,21 PRIMER_RIGHT_3=291,20 PRIMER_LEFT_3_TM=60.160 PRIMER_RIGHT_3_TM=59.936 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=133 PRIMER_PAIR_4_PENALTY=1.232805 PRIMER_LEFT_4_PENALTY=1.169021 PRIMER_RIGHT_4_PENALTY=0.063784 PRIMER_LEFT_4_SEQUENCE=CCAACTGGGGCTGTATTATCA PRIMER_RIGHT_4_SEQUENCE=GCGCGTGTGAGTATGTGAGT PRIMER_LEFT_4=154,21 PRIMER_RIGHT_4=291,20 PRIMER_LEFT_4_TM=59.831 PRIMER_RIGHT_4_TM=59.936 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=5.9000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=138 = SEQUENCE_ID=33.RHAP69FC6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTTCTTTGTCCTGTGGCTCAGTTCAAACCATTCATCTCAGAGAAAAAAGCAGAATGGTATATGATAGGCTTGAAAAGTGGGACTTAGAGGATGAATAAGGAGAATAAAGGAATAGAAGACCATACTTAGGCGGAATAACTTCTAGTGTTCAATATGGTGTCTGACACTAATTAAGTGTATATTATATTGCCTATATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAGCCACTTACAGAGAACTGAAGACATGAGGCAGAGTGAAAGGTCAAAACATTTGCAGATGAAGCAACCTTGACCCAGTGACTGCAGACTCACTTTTGTTCTATCCCATCCAGTTCCTCAAAACTCAGACGGCCTGACACTCACTAGCTAAAAATAACATGATCTTTTGCAAAGGAAATATTTAAGGCTTCCTATTA SEQUENCE_INCLUDED_REGION=31,449 SEQUENCE_TARGET=231,50 P3_COMMENT=CL is GGCGGAATAACTTCTAGTGTTCA P3_COMMENT=CR is TGACCTTTCACTCTGCCTCA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.774898 PRIMER_LEFT_0_PENALTY=3.320295 PRIMER_RIGHT_0_PENALTY=0.454603 PRIMER_LEFT_0_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA PRIMER_RIGHT_0_SEQUENCE=TGACCTTTCACTCTGCCTCA PRIMER_LEFT_0=161,23 PRIMER_RIGHT_0=328,20 PRIMER_LEFT_0_TM=59.680 PRIMER_RIGHT_0_TM=59.545 PRIMER_LEFT_0_GC_PERCENT=43.478 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=168 PRIMER_PAIR_1_PENALTY=4.098231 PRIMER_LEFT_1_PENALTY=2.659428 PRIMER_RIGHT_1_PENALTY=1.438803 PRIMER_LEFT_1_SEQUENCE=GAAGACCATACTTAGGCGGAA PRIMER_RIGHT_1_SEQUENCE=TCACTCTGCCTCATGTCTTCA PRIMER_LEFT_1=147,21 PRIMER_RIGHT_1=321,21 PRIMER_LEFT_1_TM=58.341 PRIMER_RIGHT_1_TM=59.561 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=10.2000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=175 PRIMER_PAIR_2_PENALTY=4.461250 PRIMER_LEFT_2_PENALTY=3.320295 PRIMER_RIGHT_2_PENALTY=1.140954 PRIMER_LEFT_2_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA PRIMER_RIGHT_2_SEQUENCE=CCTTTCACTCTGCCTCATGTC PRIMER_LEFT_2=161,23 PRIMER_RIGHT_2=325,21 PRIMER_LEFT_2_TM=59.680 PRIMER_RIGHT_2_TM=59.859 PRIMER_LEFT_2_GC_PERCENT=43.478 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=6.3000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=165 PRIMER_PAIR_3_PENALTY=4.461250 PRIMER_LEFT_3_PENALTY=3.320295 PRIMER_RIGHT_3_PENALTY=1.140954 PRIMER_LEFT_3_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA PRIMER_RIGHT_3_SEQUENCE=GACCTTTCACTCTGCCTCATG PRIMER_LEFT_3=161,23 PRIMER_RIGHT_3=327,21 PRIMER_LEFT_3_TM=59.680 PRIMER_RIGHT_3_TM=59.859 PRIMER_LEFT_3_GC_PERCENT=43.478 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=167 PRIMER_PAIR_4_PENALTY=4.461250 PRIMER_LEFT_4_PENALTY=3.320295 PRIMER_RIGHT_4_PENALTY=1.140954 PRIMER_LEFT_4_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA PRIMER_RIGHT_4_SEQUENCE=TGACCTTTCACTCTGCCTCAT PRIMER_LEFT_4=161,23 PRIMER_RIGHT_4=328,21 PRIMER_LEFT_4_TM=59.680 PRIMER_RIGHT_4_TM=59.859 PRIMER_LEFT_4_GC_PERCENT=43.478 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=168 = SEQUENCE_ID=35.RHAP69FD11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNTTGCTCTTACATGTCTGACCTGCTTGTCCTGGTGGTTAGAACCACTTCACATTGTTATCTTACAGTTTTATTGATGGGTTGCCCATTTGCTCATTCTTTGGGACAGAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCCCTCTTCCTCACTGGCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=19,173 SEQUENCE_TARGET=133,34 P3_COMMENT=CL is TGTCTGACCTGCTTGTCCTG P3_COMMENT=CR is ACCCAGCCAGTGAGGAAGAG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.792553 PRIMER_LEFT_0_PENALTY=0.022009 PRIMER_RIGHT_0_PENALTY=1.770544 PRIMER_LEFT_0_SEQUENCE=TGTCTGACCTGCTTGTCCTG PRIMER_RIGHT_0_SEQUENCE=ACCCAGCCAGTGAGGAAGAG PRIMER_LEFT_0=30,20 PRIMER_RIGHT_0=191,20 PRIMER_LEFT_0_TM=60.022 PRIMER_RIGHT_0_TM=61.771 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=162 PRIMER_PAIR_1_PENALTY=1.834007 PRIMER_LEFT_1_PENALTY=0.022009 PRIMER_RIGHT_1_PENALTY=1.811998 PRIMER_LEFT_1_SEQUENCE=TGTCTGACCTGCTTGTCCTG PRIMER_RIGHT_1_SEQUENCE=ACCCAGCCAGTGAGGAAGA PRIMER_LEFT_1=30,20 PRIMER_RIGHT_1=191,19 PRIMER_LEFT_1_TM=60.022 PRIMER_RIGHT_1_TM=60.812 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=1.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=162 PRIMER_PAIR_2_PENALTY=1.971300 PRIMER_LEFT_2_PENALTY=0.022009 PRIMER_RIGHT_2_PENALTY=1.949291 PRIMER_LEFT_2_SEQUENCE=TGTCTGACCTGCTTGTCCTG PRIMER_RIGHT_2_SEQUENCE=CAGCCAGTGAGGAAGAGGG PRIMER_LEFT_2=30,20 PRIMER_RIGHT_2=188,19 PRIMER_LEFT_2_TM=60.022 PRIMER_RIGHT_2_TM=60.949 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=63.158 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=159 PRIMER_PAIR_3_PENALTY=1.971300 PRIMER_LEFT_3_PENALTY=0.022009 PRIMER_RIGHT_3_PENALTY=1.949291 PRIMER_LEFT_3_SEQUENCE=TGTCTGACCTGCTTGTCCTG PRIMER_RIGHT_3_SEQUENCE=CCAGCCAGTGAGGAAGAGG PRIMER_LEFT_3=30,20 PRIMER_RIGHT_3=189,19 PRIMER_LEFT_3_TM=60.022 PRIMER_RIGHT_3_TM=60.949 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=63.158 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=4.00 PRIMER_PAIR_3_PRODUCT_SIZE=160 PRIMER_PAIR_4_PENALTY=1.971300 PRIMER_LEFT_4_PENALTY=0.022009 PRIMER_RIGHT_4_PENALTY=1.949291 PRIMER_LEFT_4_SEQUENCE=TGTCTGACCTGCTTGTCCTG PRIMER_RIGHT_4_SEQUENCE=CCCAGCCAGTGAGGAAGAG PRIMER_LEFT_4=30,20 PRIMER_RIGHT_4=190,19 PRIMER_LEFT_4_TM=60.022 PRIMER_RIGHT_4_TM=60.949 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=63.158 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=161 = SEQUENCE_ID=39.RHAP69FE6.seq SEQUENCE_TEMPLATE=CNCTNNNNNNNNNNNNNNNNNCTCTGNACACATTCGGACACANATTNTTNACACACNTTTTCTGTGACACACATACNCAGNNAGATACACACNTAACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCACNGACTGTCAANNNNNNNNNNNNNNNNNNNNNNCTATACNGACGNNNNNNNNNNNNNNCTACAAACGGGACATAAAGCGGGACACGATGANAGGCTTTTCCNCNCACACNAAGTGATANCNNACAGCNTCNCACAGACANGNNNNCGNAGTGNTTGANNCNGACGCGCNCTCNTNANNGGAGGCACGGTCTAGNNNGCGCAGGTCTNGAAGGTCGGNTTAGNGNTGNNNNTANNGNNGCNCTTACNAACGCNGANTCATANTNTNTCAGANNNNGAGGGTNTNTTATNTAAAGNNTCAANANAAANAA SEQUENCE_INCLUDED_REGION=0,442 SEQUENCE_TARGET=5,16 SEQUENCE_TARGET=99,32 SEQUENCE_TARGET=145,22 SEQUENCE_TARGET=178,14 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=40.RHAP69FF10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNACTNNTAAAAAATTGTCTTCAATATCTTTTTAAACCAATTAAATTTTTTAATTAAAATATAATTACATCACATCCTTTTTCCCTTTTGTCCCTCCAATCCCTCCCATCCTTTTCCATCACTTTCTTCCAAATTGATGGCCTCGTTTTCTTTGATGATTATTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACGCATGCACGCGCGCGCGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCAACCTTCTATATCCATTTAGTGCTGCTTGTATATGTTTTCACAGTAGAGCACTTTGTATTGGATAACCAATTAGGTTGCTCATCCCTG SEQUENCE_INCLUDED_REGION=19,361 SEQUENCE_TARGET=183,40 SEQUENCE_TARGET=248,40 P3_COMMENT=CL is CAAATTGATGGCCTCGTTTT P3_COMMENT=CR is GCAGCACTAAATGGATATAGAAGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=5.610585 PRIMER_LEFT_0_PENALTY=0.061961 PRIMER_RIGHT_0_PENALTY=5.548624 PRIMER_LEFT_0_SEQUENCE=CAAATTGATGGCCTCGTTTT PRIMER_RIGHT_0_SEQUENCE=GCAGCACTAAATGGATATAGAAGG PRIMER_LEFT_0=146,20 PRIMER_RIGHT_0=317,24 PRIMER_LEFT_0_TM=59.938 PRIMER_RIGHT_0_TM=58.451 PRIMER_LEFT_0_GC_PERCENT=40.000 PRIMER_RIGHT_0_GC_PERCENT=41.667 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.0000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=172 PRIMER_PAIR_1_PENALTY=5.740304 PRIMER_LEFT_1_PENALTY=0.191680 PRIMER_RIGHT_1_PENALTY=5.548624 PRIMER_LEFT_1_SEQUENCE=GGCCTCGTTTTCTTTGATGA PRIMER_RIGHT_1_SEQUENCE=GCAGCACTAAATGGATATAGAAGG PRIMER_LEFT_1=155,20 PRIMER_RIGHT_1=317,24 PRIMER_LEFT_1_TM=60.192 PRIMER_RIGHT_1_TM=58.451 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=41.667 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=163 PRIMER_PAIR_2_PENALTY=5.740304 PRIMER_LEFT_2_PENALTY=0.191680 PRIMER_RIGHT_2_PENALTY=5.548624 PRIMER_LEFT_2_SEQUENCE=GATGGCCTCGTTTTCTTTGA PRIMER_RIGHT_2_SEQUENCE=GCAGCACTAAATGGATATAGAAGG PRIMER_LEFT_2=152,20 PRIMER_RIGHT_2=317,24 PRIMER_LEFT_2_TM=60.192 PRIMER_RIGHT_2_TM=58.451 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=41.667 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=166 PRIMER_PAIR_3_PENALTY=5.762908 PRIMER_LEFT_3_PENALTY=0.061961 PRIMER_RIGHT_3_PENALTY=5.700947 PRIMER_LEFT_3_SEQUENCE=CAAATTGATGGCCTCGTTTT PRIMER_RIGHT_3_SEQUENCE=AGCAGCACTAAATGGATATAGAAGG PRIMER_LEFT_3=146,20 PRIMER_RIGHT_3=318,25 PRIMER_LEFT_3_TM=59.938 PRIMER_RIGHT_3_TM=59.299 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=40.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=173 PRIMER_PAIR_4_PENALTY=5.766630 PRIMER_LEFT_4_PENALTY=0.191680 PRIMER_RIGHT_4_PENALTY=5.574950 PRIMER_LEFT_4_SEQUENCE=GGCCTCGTTTTCTTTGATGA PRIMER_RIGHT_4_SEQUENCE=CATATACAAGCAGCACTAAATGGA PRIMER_LEFT_4=155,20 PRIMER_RIGHT_4=326,24 PRIMER_LEFT_4_TM=60.192 PRIMER_RIGHT_4_TM=58.425 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=37.500 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=172 = SEQUENCE_ID=40.RHAP69FF4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCACGCACACATCTGCATACTNNNNNNNNNNNNNNNNNNNNNNNNGACATACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAATGGGCCCTTTGGCTTTTTTCTGATGACCTATCACCATCTCAAGTTCAAGACCCTGCACAGACCAGGTAATTTAAGAATCATCACCTTAAATTCAAGTTGCACCTTATGATTTAATTATTTCATGGAGATGGCTTTCTCCTTTCCAGTTTTATTCTCCATAAACTGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=17,308 SEQUENCE_TARGET=17,21 SEQUENCE_TARGET=60,24 SEQUENCE_TARGET=93,60 P3_COMMENT=CL is GCACGCACACATCTGCATAC P3_COMMENT=CR is TGCAGGGTCTTGAACTTGAG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.337718 PRIMER_LEFT_0_PENALTY=1.344726 PRIMER_RIGHT_0_PENALTY=0.992992 PRIMER_LEFT_0_SEQUENCE=GCACGCACACATCTGCATAC PRIMER_RIGHT_0_SEQUENCE=TGCAGGGTCTTGAACTTGAG PRIMER_LEFT_0=39,20 PRIMER_RIGHT_0=213,20 PRIMER_LEFT_0_TM=61.345 PRIMER_RIGHT_0_TM=59.007 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=5.6000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=175 PRIMER_PAIR_1_PENALTY=2.451371 PRIMER_LEFT_1_PENALTY=1.458379 PRIMER_RIGHT_1_PENALTY=0.992992 PRIMER_LEFT_1_SEQUENCE=GCACGCACACATCTGCATA PRIMER_RIGHT_1_SEQUENCE=TGCAGGGTCTTGAACTTGAG PRIMER_LEFT_1=39,19 PRIMER_RIGHT_1=213,20 PRIMER_LEFT_1_TM=60.458 PRIMER_RIGHT_1_TM=59.007 PRIMER_LEFT_1_GC_PERCENT=52.632 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.4000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=175 PRIMER_PAIR_2_PENALTY=2.535897 PRIMER_LEFT_2_PENALTY=1.344726 PRIMER_RIGHT_2_PENALTY=1.191170 PRIMER_LEFT_2_SEQUENCE=GCACGCACACATCTGCATAC PRIMER_RIGHT_2_SEQUENCE=CAGAAAAAAGCCAAAGGGC PRIMER_LEFT_2=39,20 PRIMER_RIGHT_2=178,19 PRIMER_LEFT_2_TM=61.345 PRIMER_RIGHT_2_TM=59.809 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=47.368 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=5.6000 PRIMER_RIGHT_2_END_STABILITY=10.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=140 PRIMER_PAIR_3_PENALTY=2.649549 PRIMER_LEFT_3_PENALTY=1.458379 PRIMER_RIGHT_3_PENALTY=1.191170 PRIMER_LEFT_3_SEQUENCE=GCACGCACACATCTGCATA PRIMER_RIGHT_3_SEQUENCE=CAGAAAAAAGCCAAAGGGC PRIMER_LEFT_3=39,19 PRIMER_RIGHT_3=178,19 PRIMER_LEFT_3_TM=60.458 PRIMER_RIGHT_3_TM=59.809 PRIMER_LEFT_3_GC_PERCENT=52.632 PRIMER_RIGHT_3_GC_PERCENT=47.368 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.4000 PRIMER_RIGHT_3_END_STABILITY=10.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=140 PRIMER_PAIR_4_PENALTY=2.687253 PRIMER_LEFT_4_PENALTY=1.694261 PRIMER_RIGHT_4_PENALTY=0.992992 PRIMER_LEFT_4_SEQUENCE=CACGCACACATCTGCATACT PRIMER_RIGHT_4_SEQUENCE=TGCAGGGTCTTGAACTTGAG PRIMER_LEFT_4=40,20 PRIMER_RIGHT_4=213,20 PRIMER_LEFT_4_TM=58.306 PRIMER_RIGHT_4_TM=59.007 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=5.3000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=174 = SEQUENCE_ID=41.RHAP69FF11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNTAAGAGCTTAACAACTTATGGCAGCTTTTTAAAATCTGTCCACTTTTCTAAGTGCACTGTCTTCTTTGTCTCCCTTCTATCGTAGTTTTAAGCACATTTTCCTTCACACTTTGAGTTTTCNNNNNNNNNNNNNNNCATGACTCCTCTGCCATTATGTGACTGTTGTCCACTATGTGGCTGACCTCAATGTGGGGTTAACTCACATGACATGATTTTTTCCCCCTTTTCTCCCCTTCATCTTCCTTCTCTAGGCACCTGCTGAGATTTACAGCCTTGGGGATATTTTTTAAGCCTGAAAAGNNNNNNNNNNNNNNNNNNNNACAGNNNNNNNNNNNNNNNNNGNCANNNCGCTACNGNGNG SEQUENCE_INCLUDED_REGION=18,360 SEQUENCE_TARGET=138,15 SEQUENCE_TARGET=318,20 SEQUENCE_TARGET=342,17 P3_COMMENT=CL is GCACTGTCTTCTTTGTCTCCC P3_COMMENT=CR is TGAGGTCAGCCACATAGTGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.367400 PRIMER_LEFT_0_PENALTY=2.068459 PRIMER_RIGHT_0_PENALTY=0.298942 PRIMER_LEFT_0_SEQUENCE=GCACTGTCTTCTTTGTCTCCC PRIMER_RIGHT_0_SEQUENCE=TGAGGTCAGCCACATAGTGG PRIMER_LEFT_0=71,21 PRIMER_RIGHT_0=203,20 PRIMER_LEFT_0_TM=58.932 PRIMER_RIGHT_0_TM=59.701 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=133 PRIMER_PAIR_1_PENALTY=2.376282 PRIMER_LEFT_1_PENALTY=2.077340 PRIMER_RIGHT_1_PENALTY=0.298942 PRIMER_LEFT_1_SEQUENCE=GCACTGTCTTCTTTGTCTCCCT PRIMER_RIGHT_1_SEQUENCE=TGAGGTCAGCCACATAGTGG PRIMER_LEFT_1=71,22 PRIMER_RIGHT_1=203,20 PRIMER_LEFT_1_TM=59.923 PRIMER_RIGHT_1_TM=59.701 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=6.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=133 PRIMER_PAIR_2_PENALTY=2.494227 PRIMER_LEFT_2_PENALTY=2.345718 PRIMER_RIGHT_2_PENALTY=0.148509 PRIMER_LEFT_2_SEQUENCE=GCACATTTTCCTTCACACTTTG PRIMER_RIGHT_2_SEQUENCE=CAGCAGGTGCCTAGAGAAGG PRIMER_LEFT_2=109,22 PRIMER_RIGHT_2=278,20 PRIMER_LEFT_2_TM=59.654 PRIMER_RIGHT_2_TM=60.149 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=170 PRIMER_PAIR_3_PENALTY=2.517268 PRIMER_LEFT_3_PENALTY=2.068459 PRIMER_RIGHT_3_PENALTY=0.448810 PRIMER_LEFT_3_SEQUENCE=GCACTGTCTTCTTTGTCTCCC PRIMER_RIGHT_3_SEQUENCE=GGTCAGCCACATAGTGGACA PRIMER_LEFT_3=71,21 PRIMER_RIGHT_3=200,20 PRIMER_LEFT_3_TM=58.932 PRIMER_RIGHT_3_TM=59.551 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=130 PRIMER_PAIR_4_PENALTY=2.517268 PRIMER_LEFT_4_PENALTY=2.068459 PRIMER_RIGHT_4_PENALTY=0.448810 PRIMER_LEFT_4_SEQUENCE=GCACTGTCTTCTTTGTCTCCC PRIMER_RIGHT_4_SEQUENCE=CACATTGAGGTCAGCCACAT PRIMER_LEFT_4=71,21 PRIMER_RIGHT_4=208,20 PRIMER_LEFT_4_TM=58.932 PRIMER_RIGHT_4_TM=59.551 PRIMER_LEFT_4_GC_PERCENT=52.381 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=138 = SEQUENCE_ID=42.RHAP69FF6.seq SEQUENCE_TEMPLATE=CACTNNNNNNNNNNNNNNNNCACTGNCACACGNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCAAACCAGACAGAGAGTTGACACATTCACAAGTTCAAGACGCAAAGTGTTCTTTTCTTCGAATTACACTTCCTTGAGTTTCGCCTGGCCTTGGGTTGTTTTGAAGTTGGCAAGTCTCCTCAAAGCTGCTTTTGAGAGCCACCGTGTTCATTAGCTGGTTCACAGCTTCCTCCTACCACTTAGCTTGCCTGGAACCCTGCTGTGCAAACAGGCCTGGTGAGCTGGGATGTCTGGCACGTGCAAGGCCCAGGTTCCACCCTGGCACAGTGAGGAGGAAGCAGGCCTTGTGTGTGGACACCCAGTCCCTGTGGCACTTAGAGCCTACCNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,428 SEQUENCE_TARGET=4,16 SEQUENCE_TARGET=35,40 SEQUENCE_TARGET=402,26 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=44.RHAP69FG5.seq SEQUENCE_TEMPLATE=AANTNTATNNTTGNNAGGNNNANGNANGGAGNNNNNNNGGANNGNNNNNTANNGTNNAANNNGNTNGGGNNNTNGGNNNNANNNACNNNNTCTNAANNTCTATTNANTNCCACNTNNTGGGGNNAACCATNCANTNNCAGNTGTGCTTNNGAGGGTGGNNTTCTGATTTNAAAACCAANCAACANNAANNNATTAGANANNNNNNNTNNCACAACACNCATNANNCNNNNNNNNNNNNNNNNNNNACNCNNAAACTCCANTCACNCANAAATTATTCTTCTTCNCCAGCNANTTCATGAAANTTTTCTTCAAGAACCACATTACTTTGGGCACAAAAATAAGGCTCNTCAGGATGACAAAGAAAAATTGNACTAGGNACTGGNTTAAAAGAANAGCATAATTCAAACAATATAATGNTGGGNAAAANTGAATGGGCTGACAGATAGAAGAACTTGATCANCANGGAACANNATTTNAACTTCAAGTTAATNAAAAACNC SEQUENCE_INCLUDED_REGION=0,499 SEQUENCE_TARGET=228,14 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=01.RHAP131FA1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCNTTAAGGATTACAANACTGAACCACCAACCAANNAGCATGCATNGGNNTGACAANGNCNNNNNNNNNNNNNNNACACNCTCGNCNCNCTCNCACNCACNCNCTCTCNNNCNTCTNTATNTAGNGNGGTATANTTGNGTCNCTAAANNTAGAATGTTNNNTNNGNNATNTTTNNANANGGGGGNNGNTNACCTACANGNNCNCTNTTNGCTAACNNTTTTGTTATTGCCANTCAACTNNAANNTTTGGGNGNCNNNNTTNTTGNTCTAGANTTTTNAATGTNNANAAAAANANNNAANNTNNTAAGANTNNNNTNNGGTNNNNTNNTAAAAGGGNGTTTTTCNNCNAATAANANNGGGGNAANANNNNAGGGGGAGNNNNTNANAAAANNNGGGGNTNNTTGNANNNNNNAATANNNATAGNGTTNAANNNNAATNNNNTTCAATTNNNAAATGNNAAGNGTTNNAAAAAAANTGTTNNTNNNTTNNTTNAAANTGATGANTNTGNNTANNACNAANTTTCTNNGNNGAGAANNCCGAAGCCTCCNAAANNCTNTNAATTNGNNNAANCAANAANNNGNNGGCNNAAANAAANNATTGTTNNNCCCNNNNAAAATTNCTCNNCCNNNAANNANGACGNNNNNANNATNNNNGGNNANATNCNNTGGGGNAGCNTNAATTNNATGANNTTNNNNNACAAANNAATTNGANNANNCTA SEQUENCE_INCLUDED_REGION=23,717 SEQUENCE_TARGET=84,14 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=01.RHAP131FA7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCAGTATAAGTATGTCTGGAGATCTTTTAGAAATGAGGGGTAAATTCTGAGGGCAGGAAGGTTTTAGGGGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACTGCCTGCAAATTGGTATAGGCTGAGAAATTCAATTAATCGTATTCATCAATTTTTCACTCTGGGGGGGGAAATGAGGCAGAGATGTGGAATTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,224 SEQUENCE_TARGET=95,50 P3_COMMENT=CL is GAGGGCAGGAAGGTTTTAGG P3_COMMENT=CR is CCCCCAGAGTGAAAAATTGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.164371 PRIMER_LEFT_0_PENALTY=0.067516 PRIMER_RIGHT_0_PENALTY=0.096855 PRIMER_LEFT_0_SEQUENCE=GAGGGCAGGAAGGTTTTAGG PRIMER_RIGHT_0_SEQUENCE=CCCCCAGAGTGAAAAATTGA PRIMER_LEFT_0=70,20 PRIMER_RIGHT_0=215,20 PRIMER_LEFT_0_TM=60.068 PRIMER_RIGHT_0_TM=59.903 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=7.5000 PRIMER_RIGHT_0_END_STABILITY=6.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=146 PRIMER_PAIR_1_PENALTY=0.170721 PRIMER_LEFT_1_PENALTY=0.073866 PRIMER_RIGHT_1_PENALTY=0.096855 PRIMER_LEFT_1_SEQUENCE=ATTCTGAGGGCAGGAAGGTT PRIMER_RIGHT_1_SEQUENCE=CCCCCAGAGTGAAAAATTGA PRIMER_LEFT_1=65,20 PRIMER_RIGHT_1=215,20 PRIMER_LEFT_1_TM=60.074 PRIMER_RIGHT_1_TM=59.903 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=151 PRIMER_PAIR_2_PENALTY=0.170721 PRIMER_LEFT_2_PENALTY=0.073866 PRIMER_RIGHT_2_PENALTY=0.096855 PRIMER_LEFT_2_SEQUENCE=AATTCTGAGGGCAGGAAGGT PRIMER_RIGHT_2_SEQUENCE=CCCCCAGAGTGAAAAATTGA PRIMER_LEFT_2=64,20 PRIMER_RIGHT_2=215,20 PRIMER_LEFT_2_TM=60.074 PRIMER_RIGHT_2_TM=59.903 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=152 PRIMER_PAIR_3_PENALTY=0.193675 PRIMER_LEFT_3_PENALTY=0.096820 PRIMER_RIGHT_3_PENALTY=0.096855 PRIMER_LEFT_3_SEQUENCE=GAGGGGTAAATTCTGAGGGC PRIMER_RIGHT_3_SEQUENCE=CCCCCAGAGTGAAAAATTGA PRIMER_LEFT_3=56,20 PRIMER_RIGHT_3=215,20 PRIMER_LEFT_3_TM=59.903 PRIMER_RIGHT_3_TM=59.903 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=10.9000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=160 PRIMER_PAIR_4_PENALTY=0.519743 PRIMER_LEFT_4_PENALTY=0.422888 PRIMER_RIGHT_4_PENALTY=0.096855 PRIMER_LEFT_4_SEQUENCE=GCAGGAAGGTTTTAGGGGAA PRIMER_RIGHT_4_SEQUENCE=CCCCCAGAGTGAAAAATTGA PRIMER_LEFT_4=74,20 PRIMER_RIGHT_4=215,20 PRIMER_LEFT_4_TM=60.423 PRIMER_RIGHT_4_TM=59.903 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=142 = SEQUENCE_ID=02.RHAP131FA8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCAGTATAAGTATGTCTGGANTAATAATAGCAAAAAGTATAAAACCATGAGGGCAGGAAGGGTGGCTCCACTGGGATTACCTGCTGGAACCTCCTCCCTTTCCACAGGAACCTGAGCTTGTGACAAACTAATAAAATATGGAATTACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCAGAAGAACAGGGAGATCTTGGAGGACCCATTCCAAGCATTGTTTATTCATTCATGTGGACACCGTGACTTGGGGGCTGGAGAGAGGGTGCAGCAGTTGAGAATAGACAACTCTTCCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=22,336 SEQUENCE_TARGET=170,61 P3_COMMENT=CL is TTCCACAGGAACCTGAGCTT P3_COMMENT=CR is AACAATGCTTGGAATGGGTC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.359427 PRIMER_LEFT_0_PENALTY=0.156061 PRIMER_RIGHT_0_PENALTY=0.203367 PRIMER_LEFT_0_SEQUENCE=TTCCACAGGAACCTGAGCTT PRIMER_RIGHT_0_SEQUENCE=AACAATGCTTGGAATGGGTC PRIMER_LEFT_0=121,20 PRIMER_RIGHT_0=279,20 PRIMER_LEFT_0_TM=59.844 PRIMER_RIGHT_0_TM=59.797 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=9.1000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=159 PRIMER_PAIR_1_PENALTY=0.359427 PRIMER_LEFT_1_PENALTY=0.156061 PRIMER_RIGHT_1_PENALTY=0.203367 PRIMER_LEFT_1_SEQUENCE=TTTCCACAGGAACCTGAGCT PRIMER_RIGHT_1_SEQUENCE=AACAATGCTTGGAATGGGTC PRIMER_LEFT_1=120,20 PRIMER_RIGHT_1=279,20 PRIMER_LEFT_1_TM=59.844 PRIMER_RIGHT_1_TM=59.797 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=9.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=160 PRIMER_PAIR_2_PENALTY=0.359427 PRIMER_LEFT_2_PENALTY=0.156061 PRIMER_RIGHT_2_PENALTY=0.203367 PRIMER_LEFT_2_SEQUENCE=CTTTCCACAGGAACCTGAGC PRIMER_RIGHT_2_SEQUENCE=AACAATGCTTGGAATGGGTC PRIMER_LEFT_2=119,20 PRIMER_RIGHT_2=279,20 PRIMER_LEFT_2_TM=59.844 PRIMER_RIGHT_2_TM=59.797 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.1000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=161 PRIMER_PAIR_3_PENALTY=0.434225 PRIMER_LEFT_3_PENALTY=0.156061 PRIMER_RIGHT_3_PENALTY=0.278164 PRIMER_LEFT_3_SEQUENCE=TTCCACAGGAACCTGAGCTT PRIMER_RIGHT_3_SEQUENCE=ATGGGTCCTCCAAGATCTCC PRIMER_LEFT_3=121,20 PRIMER_RIGHT_3=266,20 PRIMER_LEFT_3_TM=59.844 PRIMER_RIGHT_3_TM=60.278 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=5.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=146 PRIMER_PAIR_4_PENALTY=0.434225 PRIMER_LEFT_4_PENALTY=0.156061 PRIMER_RIGHT_4_PENALTY=0.278164 PRIMER_LEFT_4_SEQUENCE=TTTCCACAGGAACCTGAGCT PRIMER_RIGHT_4_SEQUENCE=ATGGGTCCTCCAAGATCTCC PRIMER_LEFT_4=120,20 PRIMER_RIGHT_4=266,20 PRIMER_LEFT_4_TM=59.844 PRIMER_RIGHT_4_TM=60.278 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=147 = SEQUENCE_ID=04.RHAP131FB7.seq SEQUENCE_TEMPLATE=TCTAGGACCCCCCTATTTGGGCTCTTCTCNCCGGGTCCTCTGAAAGAACAGCCAGTCTTCCTTTACACTGGAAAGCAGAGAGTGAGTCATTTAGAGGGTCTTATGACCTGCTTCCTGGGAAAGTCAAGATGTTCTTCCTATTTTTAATGATCTACTTTAAATAGGGAAAAATACAAGGACAGAGAAGATTATTTTTGCTTGTGACATTTCCTTCACCAAATTCCTGCAGCTTAAAATACTAGTGTGCCAAAGTATGGTAAAATGTTCTTGAGTTCATCAATACAACTATTTTCTTAAAAGAATTCTTTATCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGGGGTGAGCGAGCAGTCTCTCTGTGCATGGAAGAATTGGGGGATTGAACCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,422 SEQUENCE_TARGET=313,50 P3_COMMENT=CL is CCAAATTCCTGCAGCTTAAAA P3_COMMENT=CR is AGAGAGACTGCTCGCTCACC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.738003 PRIMER_LEFT_0_PENALTY=1.628243 PRIMER_RIGHT_0_PENALTY=0.109760 PRIMER_LEFT_0_SEQUENCE=CCAAATTCCTGCAGCTTAAAA PRIMER_RIGHT_0_SEQUENCE=AGAGAGACTGCTCGCTCACC PRIMER_LEFT_0=215,21 PRIMER_RIGHT_0=388,20 PRIMER_LEFT_0_TM=59.372 PRIMER_RIGHT_0_TM=59.890 PRIMER_LEFT_0_GC_PERCENT=38.095 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.6000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=174 PRIMER_PAIR_1_PENALTY=2.065161 PRIMER_LEFT_1_PENALTY=1.955401 PRIMER_RIGHT_1_PENALTY=0.109760 PRIMER_LEFT_1_SEQUENCE=CCAAATTCCTGCAGCTTAAA PRIMER_RIGHT_1_SEQUENCE=AGAGAGACTGCTCGCTCACC PRIMER_LEFT_1=215,20 PRIMER_RIGHT_1=388,20 PRIMER_LEFT_1_TM=58.045 PRIMER_RIGHT_1_TM=59.890 PRIMER_LEFT_1_GC_PERCENT=40.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=174 PRIMER_PAIR_2_PENALTY=2.221575 PRIMER_LEFT_2_PENALTY=2.111815 PRIMER_RIGHT_2_PENALTY=0.109760 PRIMER_LEFT_2_SEQUENCE=ACCAAATTCCTGCAGCTTAAA PRIMER_RIGHT_2_SEQUENCE=AGAGAGACTGCTCGCTCACC PRIMER_LEFT_2=214,21 PRIMER_RIGHT_2=388,20 PRIMER_LEFT_2_TM=58.888 PRIMER_RIGHT_2_TM=59.890 PRIMER_LEFT_2_GC_PERCENT=38.095 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=175 PRIMER_PAIR_3_PENALTY=2.237688 PRIMER_LEFT_3_PENALTY=2.127927 PRIMER_RIGHT_3_PENALTY=0.109760 PRIMER_LEFT_3_SEQUENCE=ACCAAATTCCTGCAGCTTAAAA PRIMER_RIGHT_3_SEQUENCE=AGAGAGACTGCTCGCTCACC PRIMER_LEFT_3=214,22 PRIMER_RIGHT_3=388,20 PRIMER_LEFT_3_TM=60.128 PRIMER_RIGHT_3_TM=59.890 PRIMER_LEFT_3_GC_PERCENT=36.364 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=175 PRIMER_PAIR_4_PENALTY=2.465353 PRIMER_LEFT_4_PENALTY=2.355593 PRIMER_RIGHT_4_PENALTY=0.109760 PRIMER_LEFT_4_SEQUENCE=CCAAATTCCTGCAGCTTAAAAT PRIMER_RIGHT_4_SEQUENCE=AGAGAGACTGCTCGCTCACC PRIMER_LEFT_4=215,22 PRIMER_RIGHT_4=388,20 PRIMER_LEFT_4_TM=59.644 PRIMER_RIGHT_4_TM=59.890 PRIMER_LEFT_4_GC_PERCENT=36.364 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=7.2000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=174 = SEQUENCE_ID=08.RHAP131FC2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCAGGATATTTTGCCCTTGCAAGGAACATACTACACAGCTCATGCCAAGTTTTTCATGGTTTTTGTGAGAAAATTTTAGGTGCAGCAAAAGAGACGATTGGTTACATTCTCACAGCTGATTAGCTCCAACACAGAGTGGTATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGGGTGGGAAGAGAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=22,210 SEQUENCE_TARGET=167,46 P3_COMMENT=CL is TGCAGCAAAAGAGACGATTG P3_COMMENT=CR is CCTTCTCTTCCCACCCATC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.689198 PRIMER_LEFT_0_PENALTY=0.134363 PRIMER_RIGHT_0_PENALTY=1.554836 PRIMER_LEFT_0_SEQUENCE=TGCAGCAAAAGAGACGATTG PRIMER_RIGHT_0_SEQUENCE=CCTTCTCTTCCCACCCATC PRIMER_LEFT_0=102,20 PRIMER_RIGHT_0=231,19 PRIMER_LEFT_0_TM=60.134 PRIMER_RIGHT_0_TM=59.445 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=57.895 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=130 PRIMER_PAIR_1_PENALTY=1.960552 PRIMER_LEFT_1_PENALTY=0.405717 PRIMER_RIGHT_1_PENALTY=1.554836 PRIMER_LEFT_1_SEQUENCE=GGTGCAGCAAAAGAGACGAT PRIMER_RIGHT_1_SEQUENCE=CCTTCTCTTCCCACCCATC PRIMER_LEFT_1=100,20 PRIMER_RIGHT_1=231,19 PRIMER_LEFT_1_TM=60.406 PRIMER_RIGHT_1_TM=59.445 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=57.895 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.0000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=132 PRIMER_PAIR_2_PENALTY=2.036792 PRIMER_LEFT_2_PENALTY=0.481956 PRIMER_RIGHT_2_PENALTY=1.554836 PRIMER_LEFT_2_SEQUENCE=TCATGCCAAGTTTTTCATGG PRIMER_RIGHT_2_SEQUENCE=CCTTCTCTTCCCACCCATC PRIMER_LEFT_2=61,20 PRIMER_RIGHT_2=231,19 PRIMER_LEFT_2_TM=59.518 PRIMER_RIGHT_2_TM=59.445 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=8.1000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=171 PRIMER_PAIR_3_PENALTY=2.334969 PRIMER_LEFT_3_PENALTY=0.780133 PRIMER_RIGHT_3_PENALTY=1.554836 PRIMER_LEFT_3_SEQUENCE=TAGGTGCAGCAAAAGAGACG PRIMER_RIGHT_3_SEQUENCE=CCTTCTCTTCCCACCCATC PRIMER_LEFT_3=98,20 PRIMER_RIGHT_3=231,19 PRIMER_LEFT_3_TM=59.220 PRIMER_RIGHT_3_TM=59.445 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=57.895 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.1000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=134 PRIMER_PAIR_4_PENALTY=2.469079 PRIMER_LEFT_4_PENALTY=0.914244 PRIMER_RIGHT_4_PENALTY=1.554836 PRIMER_LEFT_4_SEQUENCE=GCAGCAAAAGAGACGATTGG PRIMER_RIGHT_4_SEQUENCE=CCTTCTCTTCCCACCCATC PRIMER_LEFT_4=103,20 PRIMER_RIGHT_4=231,19 PRIMER_LEFT_4_TM=60.914 PRIMER_RIGHT_4_TM=59.445 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=57.895 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=129 = SEQUENCE_ID=08.RHAP131FC8.seq SEQUENCE_TEMPLATE=AAAAANNGNTTAGAGGATCCCCCATAGAAGCTTCTACTATACCAGCTCTAGTAGAAGGGGACAAGCCTTAGTTTATGCAACTGAGTTCATGGCACGGGATTGTAAATGGTACATGAGTCTTATGTTCAAGCATTATTTCTATAAAATCTCACTTTTTAAGTCCAGTGAGTAAGCTGTGAGCTCCGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTCCCAGCTACTTGGGAGGCTCAGTTCCATGATGTTTATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,293 SEQUENCE_TARGET=187,64 P3_COMMENT=CL is TCCAGTGAGTAAGCTGTGAGCT P3_COMMENT=CR is CATCATGGAACTGAGCCTCC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=3.317152 PRIMER_LEFT_0_PENALTY=2.694680 PRIMER_RIGHT_0_PENALTY=0.622472 PRIMER_LEFT_0_SEQUENCE=CCAGTGAGTAAGCTGTGAGCTC PRIMER_RIGHT_0_SEQUENCE=CATCATGGAACTGAGCCTCC PRIMER_LEFT_0=161,22 PRIMER_RIGHT_0=285,20 PRIMER_LEFT_0_TM=59.305 PRIMER_RIGHT_0_TM=60.622 PRIMER_LEFT_0_GC_PERCENT=54.545 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=9.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=125 PRIMER_PAIR_1_PENALTY=3.317152 PRIMER_LEFT_1_PENALTY=2.694680 PRIMER_RIGHT_1_PENALTY=0.622472 PRIMER_LEFT_1_SEQUENCE=TCCAGTGAGTAAGCTGTGAGCT PRIMER_RIGHT_1_SEQUENCE=CATCATGGAACTGAGCCTCC PRIMER_LEFT_1=160,22 PRIMER_RIGHT_1=285,20 PRIMER_LEFT_1_TM=59.305 PRIMER_RIGHT_1_TM=60.622 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=5.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=126 PRIMER_PAIR_2_PENALTY=3.364888 PRIMER_LEFT_2_PENALTY=2.742415 PRIMER_RIGHT_2_PENALTY=0.622472 PRIMER_LEFT_2_SEQUENCE=TCCAGTGAGTAAGCTGTGAGC PRIMER_RIGHT_2_SEQUENCE=CATCATGGAACTGAGCCTCC PRIMER_LEFT_2=160,21 PRIMER_RIGHT_2=285,20 PRIMER_LEFT_2_TM=58.258 PRIMER_RIGHT_2_TM=60.622 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=9.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=126 PRIMER_PAIR_3_PENALTY=3.457555 PRIMER_LEFT_3_PENALTY=2.835083 PRIMER_RIGHT_3_PENALTY=0.622472 PRIMER_LEFT_3_SEQUENCE=GTCCAGTGAGTAAGCTGTGAGC PRIMER_RIGHT_3_SEQUENCE=CATCATGGAACTGAGCCTCC PRIMER_LEFT_3=159,22 PRIMER_RIGHT_3=285,20 PRIMER_LEFT_3_TM=59.165 PRIMER_RIGHT_3_TM=60.622 PRIMER_LEFT_3_GC_PERCENT=54.545 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=127 PRIMER_PAIR_4_PENALTY=3.548510 PRIMER_LEFT_4_PENALTY=1.208168 PRIMER_RIGHT_4_PENALTY=2.340342 PRIMER_LEFT_4_SEQUENCE=GTGAGTAAGCTGTGAGCTCCG PRIMER_RIGHT_4_SEQUENCE=AAACATCATGGAACTGAGCCT PRIMER_LEFT_4=164,21 PRIMER_RIGHT_4=288,21 PRIMER_LEFT_4_TM=60.208 PRIMER_RIGHT_4_TM=58.660 PRIMER_LEFT_4_GC_PERCENT=57.143 PRIMER_RIGHT_4_GC_PERCENT=42.857 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_END_STABILITY=9.9000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=125 = SEQUENCE_ID=10.RHAP131FD1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNGAACTATATCCCCAGCTCTTAGGAATGGAAATTCAGGATTCCAAAAGGAAGCAAAAAATAAAGCCTCACCCATAACCCCACATTCTTTGTGCACGCCCCTCTCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGTGAGAATTGAACCCGGAGTCTGTCTAAGCTAAGTGNNNNNNNNNNNNNNGAGCCACCGCTAGCCACAGCGTTGTTTAATTACCTGTTGCTCTAGTTCAGAACTTTTACTGTGGTTAACCATGTTGAGCTACAGTGTTTCACTGTAACCACTTCTCATGGCTCGCTCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,326 SEQUENCE_TARGET=118,46 SEQUENCE_TARGET=203,14 P3_COMMENT=CL is CAGGATTCCAAAAGGAAGCA P3_COMMENT=CR is GACTCCGGGTTCAATTCTCA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.233803 PRIMER_LEFT_0_PENALTY=0.183944 PRIMER_RIGHT_0_PENALTY=0.049859 PRIMER_LEFT_0_SEQUENCE=CAGGATTCCAAAAGGAAGCA PRIMER_RIGHT_0_SEQUENCE=GACTCCGGGTTCAATTCTCA PRIMER_LEFT_0=44,20 PRIMER_RIGHT_0=187,20 PRIMER_LEFT_0_TM=60.184 PRIMER_RIGHT_0_TM=60.050 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=6.7000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=144 PRIMER_PAIR_1_PENALTY=0.785453 PRIMER_LEFT_1_PENALTY=0.735594 PRIMER_RIGHT_1_PENALTY=0.049859 PRIMER_LEFT_1_SEQUENCE=CCCAGCTCTTAGGAATGGAA PRIMER_RIGHT_1_SEQUENCE=GACTCCGGGTTCAATTCTCA PRIMER_LEFT_1=21,20 PRIMER_RIGHT_1=187,20 PRIMER_LEFT_1_TM=59.264 PRIMER_RIGHT_1_TM=60.050 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=5.00 PRIMER_PAIR_1_PRODUCT_SIZE=167 PRIMER_PAIR_2_PENALTY=0.805400 PRIMER_LEFT_2_PENALTY=0.755541 PRIMER_RIGHT_2_PENALTY=0.049859 PRIMER_LEFT_2_SEQUENCE=TCAGGATTCCAAAAGGAAGC PRIMER_RIGHT_2_SEQUENCE=GACTCCGGGTTCAATTCTCA PRIMER_LEFT_2=43,20 PRIMER_RIGHT_2=187,20 PRIMER_LEFT_2_TM=59.244 PRIMER_RIGHT_2_TM=60.050 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=145 PRIMER_PAIR_3_PENALTY=0.915016 PRIMER_LEFT_3_PENALTY=0.865157 PRIMER_RIGHT_3_PENALTY=0.049859 PRIMER_LEFT_3_SEQUENCE=GGATTCCAAAAGGAAGCAAA PRIMER_RIGHT_3_SEQUENCE=GACTCCGGGTTCAATTCTCA PRIMER_LEFT_3=46,20 PRIMER_RIGHT_3=187,20 PRIMER_LEFT_3_TM=59.135 PRIMER_RIGHT_3_TM=60.050 PRIMER_LEFT_3_GC_PERCENT=40.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=8.8000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=142 PRIMER_PAIR_4_PENALTY=1.046923 PRIMER_LEFT_4_PENALTY=0.183944 PRIMER_RIGHT_4_PENALTY=0.862979 PRIMER_LEFT_4_SEQUENCE=CAGGATTCCAAAAGGAAGCA PRIMER_RIGHT_4_SEQUENCE=CAGACTCCGGGTTCAATTCT PRIMER_LEFT_4=44,20 PRIMER_RIGHT_4=189,20 PRIMER_LEFT_4_TM=60.184 PRIMER_RIGHT_4_TM=59.137 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=146 = SEQUENCE_ID=10.RHAP131FD7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCACTACTGCAACAGTGTTTCCACTTCAGCATGTGTGGGTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATATGGGTATCTCTATCTGTATCTGTGTATCTCCTTTATTTATTGATGACTTTAGATGCAGATTTTTTAGGGTGGAGGATGTCCTTGTTTTGATCACATGTTATTTGAAGAACACAATTCCCAGCTAAAAGAATTTAGCTATTTTAGTTGTATCTGCAATGAAAATGCAACTTTTCAGTGAAATTAGCCACGGGTTGAGTTTATGATGAGATGCTCTTTGGAAGACAGAAATGTACTCTCATTTATAAGTTCAAGTCTCATTGAGAATACTCCAAGATTTATTTGTTTACTTTTCAAGAAAAAAAATTGATTTACAGTCTCGAACACAAGAACAAAGCCACAGACAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=12,433 SEQUENCE_TARGET=53,42 P3_COMMENT=CL is TTCCACTTCAGCATGTGTGG P3_COMMENT=CR is CAAGGACATCCTCCACCCTA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.802713 PRIMER_LEFT_0_PENALTY=0.722400 PRIMER_RIGHT_0_PENALTY=0.080313 PRIMER_LEFT_0_SEQUENCE=TTCCACTTCAGCATGTGTGG PRIMER_RIGHT_0_SEQUENCE=CAAGGACATCCTCCACCCTA PRIMER_LEFT_0=29,20 PRIMER_RIGHT_0=182,20 PRIMER_LEFT_0_TM=60.722 PRIMER_RIGHT_0_TM=59.920 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=8.7000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=3.00 PRIMER_PAIR_0_PRODUCT_SIZE=154 PRIMER_PAIR_1_PENALTY=1.303350 PRIMER_LEFT_1_PENALTY=1.223037 PRIMER_RIGHT_1_PENALTY=0.080313 PRIMER_LEFT_1_SEQUENCE=CACTTCAGCATGTGTGGGTG PRIMER_RIGHT_1_SEQUENCE=CAAGGACATCCTCCACCCTA PRIMER_LEFT_1=32,20 PRIMER_RIGHT_1=182,20 PRIMER_LEFT_1_TM=61.223 PRIMER_RIGHT_1_TM=59.920 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=8.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=151 PRIMER_PAIR_2_PENALTY=1.411547 PRIMER_LEFT_2_PENALTY=1.331234 PRIMER_RIGHT_2_PENALTY=0.080313 PRIMER_LEFT_2_SEQUENCE=TGCAACAGTGTTTCCACTTCA PRIMER_RIGHT_2_SEQUENCE=CAAGGACATCCTCCACCCTA PRIMER_LEFT_2=18,21 PRIMER_RIGHT_2=182,20 PRIMER_LEFT_2_TM=60.331 PRIMER_RIGHT_2_TM=59.920 PRIMER_LEFT_2_GC_PERCENT=42.857 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=8.7000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=165 PRIMER_PAIR_3_PENALTY=1.492036 PRIMER_LEFT_3_PENALTY=0.722400 PRIMER_RIGHT_3_PENALTY=0.769636 PRIMER_LEFT_3_SEQUENCE=TTCCACTTCAGCATGTGTGG PRIMER_RIGHT_3_SEQUENCE=ACAAGGACATCCTCCACCCT PRIMER_LEFT_3=29,20 PRIMER_RIGHT_3=183,20 PRIMER_LEFT_3_TM=60.722 PRIMER_RIGHT_3_TM=60.770 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=9.1000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=155 PRIMER_PAIR_4_PENALTY=1.730779 PRIMER_LEFT_4_PENALTY=1.650466 PRIMER_RIGHT_4_PENALTY=0.080313 PRIMER_LEFT_4_SEQUENCE=CAGTGTTTCCACTTCAGCATG PRIMER_RIGHT_4_SEQUENCE=CAAGGACATCCTCCACCCTA PRIMER_LEFT_4=23,21 PRIMER_RIGHT_4=182,20 PRIMER_LEFT_4_TM=59.350 PRIMER_RIGHT_4_TM=59.920 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=8.4000 PRIMER_RIGHT_4_END_STABILITY=8.7000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=160 = SEQUENCE_ID=11.RHAP131FD2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNCCAACCATCAACCGAAGCACAAGAACAGACGTGCGTCTTTTGGCTCGCAGTGATTTTTAATGGTAGTCATCCGGACATACGACTTAGATACAATGAGACTACAAACTAACAGACAAGATTCGGGCAAAGCAGAAAAACAATGAAGCCAGCATCCTGATCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGCATGCCTGCCTGTCAATTGTGAGGTAAGACAGGAAGACTTGGTCTCCTGCCAGATCTCCATATCTTCCCGCTCTCCTTTAACTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=22,297 SEQUENCE_TARGET=183,45 P3_COMMENT=CL is AAACAATGAAGCCAGCATCC P3_COMMENT=CR is TCTGGCAGGAGACCAAGTCT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.095381 PRIMER_LEFT_0_PENALTY=0.080962 PRIMER_RIGHT_0_PENALTY=0.014419 PRIMER_LEFT_0_SEQUENCE=AAACAATGAAGCCAGCATCC PRIMER_RIGHT_0_SEQUENCE=TCTGGCAGGAGACCAAGTCT PRIMER_LEFT_0=156,20 PRIMER_RIGHT_0=285,20 PRIMER_LEFT_0_TM=60.081 PRIMER_RIGHT_0_TM=59.986 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=5.00 PRIMER_LEFT_0_END_STABILITY=8.1000 PRIMER_RIGHT_0_END_STABILITY=6.1000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=130 PRIMER_PAIR_1_PENALTY=0.203165 PRIMER_LEFT_1_PENALTY=0.188746 PRIMER_RIGHT_1_PENALTY=0.014419 PRIMER_LEFT_1_SEQUENCE=ATGAAGCCAGCATCCTGATC PRIMER_RIGHT_1_SEQUENCE=TCTGGCAGGAGACCAAGTCT PRIMER_LEFT_1=161,20 PRIMER_RIGHT_1=285,20 PRIMER_LEFT_1_TM=60.189 PRIMER_RIGHT_1_TM=59.986 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=6.6000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=125 PRIMER_PAIR_2_PENALTY=0.314662 PRIMER_LEFT_2_PENALTY=0.300243 PRIMER_RIGHT_2_PENALTY=0.014419 PRIMER_LEFT_2_SEQUENCE=AACAATGAAGCCAGCATCCT PRIMER_RIGHT_2_SEQUENCE=TCTGGCAGGAGACCAAGTCT PRIMER_LEFT_2=157,20 PRIMER_RIGHT_2=285,20 PRIMER_LEFT_2_TM=59.700 PRIMER_RIGHT_2_TM=59.986 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=7.8000 PRIMER_RIGHT_2_END_STABILITY=6.1000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=129 PRIMER_PAIR_3_PENALTY=0.355941 PRIMER_LEFT_3_PENALTY=0.341522 PRIMER_RIGHT_3_PENALTY=0.014419 PRIMER_LEFT_3_SEQUENCE=AATGAAGCCAGCATCCTGAT PRIMER_RIGHT_3_SEQUENCE=TCTGGCAGGAGACCAAGTCT PRIMER_LEFT_3=160,20 PRIMER_RIGHT_3=285,20 PRIMER_LEFT_3_TM=59.658 PRIMER_RIGHT_3_TM=59.986 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=6.1000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=126 PRIMER_PAIR_4_PENALTY=0.430002 PRIMER_LEFT_4_PENALTY=0.080962 PRIMER_RIGHT_4_PENALTY=0.349041 PRIMER_LEFT_4_SEQUENCE=AAACAATGAAGCCAGCATCC PRIMER_RIGHT_4_SEQUENCE=GAGATCTGGCAGGAGACCAA PRIMER_LEFT_4=156,20 PRIMER_RIGHT_4=289,20 PRIMER_LEFT_4_TM=60.081 PRIMER_RIGHT_4_TM=60.349 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=134 = SEQUENCE_ID=13.RHAP131FE1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCATGCCTGGAAGCCACATTGACTTTATATTGAAATAATTCTTTTTTAAAAAGTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCGCATGTGTGCTCATGCCCTTGTGTGCATGATAAGTGTATGTAGGCTTTTGTGCCACAGCAAATGTGTAGAAGTCAAAGGAAAACATTCTGAAACGAGTTCTATCTACTTTTCTGTGGATTCCAGGGACTGAACCTGTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,241 SEQUENCE_TARGET=77,38 P3_COMMENT=CL is CCTGGAAGCCACATTGACTT P3_COMMENT=CR is TATCATGCACACAAGGGCAT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.155829 PRIMER_LEFT_0_PENALTY=0.111077 PRIMER_RIGHT_0_PENALTY=0.044752 PRIMER_LEFT_0_SEQUENCE=CCTGGAAGCCACATTGACTT PRIMER_RIGHT_0_SEQUENCE=TATCATGCACACAAGGGCAT PRIMER_LEFT_0=25,20 PRIMER_RIGHT_0=151,20 PRIMER_LEFT_0_TM=60.111 PRIMER_RIGHT_0_TM=59.955 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=6.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=9.6000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=127 PRIMER_PAIR_1_PENALTY=0.206583 PRIMER_LEFT_1_PENALTY=0.111077 PRIMER_RIGHT_1_PENALTY=0.095506 PRIMER_LEFT_1_SEQUENCE=CCTGGAAGCCACATTGACTT PRIMER_RIGHT_1_SEQUENCE=CTGTGGCACAAAAGCCTACA PRIMER_LEFT_1=25,20 PRIMER_RIGHT_1=177,20 PRIMER_LEFT_1_TM=60.111 PRIMER_RIGHT_1_TM=59.904 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=6.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=5.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=153 PRIMER_PAIR_2_PENALTY=0.268317 PRIMER_LEFT_2_PENALTY=0.111077 PRIMER_RIGHT_2_PENALTY=0.157240 PRIMER_LEFT_2_SEQUENCE=CCTGGAAGCCACATTGACTT PRIMER_RIGHT_2_SEQUENCE=ACATTTGCTGTGGCACAAAA PRIMER_LEFT_2=25,20 PRIMER_RIGHT_2=184,20 PRIMER_LEFT_2_TM=60.111 PRIMER_RIGHT_2_TM=60.157 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=5.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=160 PRIMER_PAIR_3_PENALTY=0.404572 PRIMER_LEFT_3_PENALTY=0.111077 PRIMER_RIGHT_3_PENALTY=0.293496 PRIMER_LEFT_3_SEQUENCE=CCTGGAAGCCACATTGACTT PRIMER_RIGHT_3_SEQUENCE=CATTTGCTGTGGCACAAAAG PRIMER_LEFT_3=25,20 PRIMER_RIGHT_3=183,20 PRIMER_LEFT_3_TM=60.111 PRIMER_RIGHT_3_TM=60.293 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=7.3000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=159 PRIMER_PAIR_4_PENALTY=0.912165 PRIMER_LEFT_4_PENALTY=0.111077 PRIMER_RIGHT_4_PENALTY=0.801088 PRIMER_LEFT_4_SEQUENCE=CCTGGAAGCCACATTGACTT PRIMER_RIGHT_4_SEQUENCE=TGTGGCACAAAAGCCTACAT PRIMER_LEFT_4=25,20 PRIMER_RIGHT_4=176,20 PRIMER_LEFT_4_TM=60.111 PRIMER_RIGHT_4_TM=59.199 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=5.6000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=152 = SEQUENCE_ID=13.RHAP131FE7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCTTTTCAAATGTGGAGCCTACTCTAACTTTCAGTCCCTGGGTCTCTCTCAAATATTTTCNNNNNNNNNNNNNNNNNNNNNNGTTTTTNNNNNNNNNNNNNNNNNNNNNNNGNNTNNNNCTCTCNNNNNNNCNCNCNNNNNNNNNANNTCNCTCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANATAGNNNAAANAANCAANAGANCCCNAGAAAAAGTGCACATCNATGCCAACATACAGNAAAAAAAACATTTNGACACACANAAAGAATANAAAACANANNTGTGNNNNATCAANGGTATTTTNGAACCNAACACGAATGNGTGANCANNGNNCANNANCGNTGCTCCNNTGTGAAATTGTTANCAGCTCANTNTTCCTGCNGTAATAATANNTACCCGCAGAATNNTNTACAAAACATGGGGGNCGNAAACATATGNGTNTTACANNCNGGNGTGGCTNNNGNGTGAGCTNACANANNNNNGGNGGGGNAGCGCTCTCNGGNCANCTGCCTNNNGGNANNNNCANCGCGCCNGNANATGNAGNANNCGAANNCGCGNNGGNANNNNNTGTCTNNNAANAGGNANAAGGGGANNNCTTNTTTCCCANAGNGGCNGGCNNNANANNTNNNNNACCGGCCACACTGANNTCCCAAANNNC SEQUENCE_INCLUDED_REGION=27,677 SEQUENCE_TARGET=90,22 SEQUENCE_TARGET=118,22 SEQUENCE_TARGET=189,36 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=14.RHAP131FE8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNCCAGGAACCCAGCCAGTTTTCAAAGAATACTTACCATGCCATACAAGTCAGAACCTGAACCTAGGCTTTCAAGCTGTGTAAATCAATGTTCAATTGACTGTAGTAGGAGACCCACAGACTCTATTAGAAGCATTGTAGGACAATGCTAGATTTTCTGTTCTAATGTTCTTCAGATTTAGAACAACGATGACAACAGCATTAGTGTAATAGTGTTAACTGAGTACTGTAGTGCAAGAGTGCCTGCATTCCCACATACAGTATTCATAGCTTTTGGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCCAGTTTAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=25,321 SEQUENCE_TARGET=301,32 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=15.RHAP131FE3.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCTGTTTGACAGCAGGCAGAAGCTCAGGAACAATTTGTGTGGGAGTCCCAGAGGGGAGCAGGGTGACTGAAAATCCTCTGGGCTGTTACAGAACGGAGTTTGGAATCAAAGTAACCAGAACATTCAGCTCAGTAAGAGCTTAAAAGGACATTTCATCCCCCTTAAAGACAGCTAATAAAAATAGATTTGCAGTCAGTTTTCATTAGCCAAAGTGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCCGCGTGCATAGGGTGTCTTCCTCAGCTGCTCTGCTCACCTTATGTTTTGAGACAGTGTCTGTCTTTGAACCTGTAGTTCAGCTTTTTCTACTAGGCTGGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=12,368 SEQUENCE_TARGET=229,38 P3_COMMENT=CL is AAAAGGACATTTCATCCCCC P3_COMMENT=CR is AGGAAGACACCCTATGCACG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.137982 PRIMER_LEFT_0_PENALTY=0.004242 PRIMER_RIGHT_0_PENALTY=0.133741 PRIMER_LEFT_0_SEQUENCE=AAAAGGACATTTCATCCCCC PRIMER_RIGHT_0_SEQUENCE=AGGAAGACACCCTATGCACG PRIMER_LEFT_0=153,20 PRIMER_RIGHT_0=298,20 PRIMER_LEFT_0_TM=59.996 PRIMER_RIGHT_0_TM=60.134 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_END_STABILITY=9.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=146 PRIMER_PAIR_1_PENALTY=0.147644 PRIMER_LEFT_1_PENALTY=0.004242 PRIMER_RIGHT_1_PENALTY=0.143403 PRIMER_LEFT_1_SEQUENCE=AAAAGGACATTTCATCCCCC PRIMER_RIGHT_1_SEQUENCE=GAGCAGCTGAGGAAGACACC PRIMER_LEFT_1=153,20 PRIMER_RIGHT_1=307,20 PRIMER_LEFT_1_TM=59.996 PRIMER_RIGHT_1_TM=60.143 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=12.4000 PRIMER_RIGHT_1_END_STABILITY=7.6000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=155 PRIMER_PAIR_2_PENALTY=0.444387 PRIMER_LEFT_2_PENALTY=0.004242 PRIMER_RIGHT_2_PENALTY=0.440146 PRIMER_LEFT_2_SEQUENCE=AAAAGGACATTTCATCCCCC PRIMER_RIGHT_2_SEQUENCE=GCAGAGCAGCTGAGGAAGAC PRIMER_LEFT_2=153,20 PRIMER_RIGHT_2=310,20 PRIMER_LEFT_2_TM=59.996 PRIMER_RIGHT_2_TM=60.440 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=12.4000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=158 PRIMER_PAIR_3_PENALTY=0.505105 PRIMER_LEFT_3_PENALTY=0.371364 PRIMER_RIGHT_3_PENALTY=0.133741 PRIMER_LEFT_3_SEQUENCE=AAGGACATTTCATCCCCCTT PRIMER_RIGHT_3_SEQUENCE=AGGAAGACACCCTATGCACG PRIMER_LEFT_3=155,20 PRIMER_RIGHT_3=298,20 PRIMER_LEFT_3_TM=59.629 PRIMER_RIGHT_3_TM=60.134 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=9.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=144 PRIMER_PAIR_4_PENALTY=0.505105 PRIMER_LEFT_4_PENALTY=0.371364 PRIMER_RIGHT_4_PENALTY=0.133741 PRIMER_LEFT_4_SEQUENCE=AAAGGACATTTCATCCCCCT PRIMER_RIGHT_4_SEQUENCE=AGGAAGACACCCTATGCACG PRIMER_LEFT_4=154,20 PRIMER_RIGHT_4=298,20 PRIMER_LEFT_4_TM=59.629 PRIMER_RIGHT_4_TM=60.134 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=10.9000 PRIMER_RIGHT_4_END_STABILITY=9.9000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=145 = SEQUENCE_ID=15.RHAP131FE9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNCCTTTCCATTGTGAGACACTCATTGTTGGACTAGCCCAACCATAGCCTGCAAGCCATTCTAATAAAAATTGCTTTAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCATGAGCAGGAGGATGGAAACATGATGCCCTTAATTCAATGAAGTCTAAACAATATATAGACAGTTGCTATCAAGCTCTAGGAAAACAATCAGCACGACTTATTTGGGAATAAATAGATCACAAGAGTCTCTTGAGTCAACTTACAAGAGTCCCTAGCCCATGTTTCATGGATGCTGCACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=26,309 SEQUENCE_TARGET=104,46 P3_COMMENT=CL is TGGACTAGCCCAACCATAGC P3_COMMENT=CR is AAGGGCATCATGTTTCCATC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.339571 PRIMER_LEFT_0_PENALTY=0.095771 PRIMER_RIGHT_0_PENALTY=0.243800 PRIMER_LEFT_0_SEQUENCE=TGGACTAGCCCAACCATAGC PRIMER_RIGHT_0_SEQUENCE=AAGGGCATCATGTTTCCATC PRIMER_LEFT_0=52,20 PRIMER_RIGHT_0=183,20 PRIMER_LEFT_0_TM=60.096 PRIMER_RIGHT_0_TM=59.756 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=8.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=7.1000 PRIMER_RIGHT_0_END_STABILITY=8.1000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=132 PRIMER_PAIR_1_PENALTY=0.385209 PRIMER_LEFT_1_PENALTY=0.095771 PRIMER_RIGHT_1_PENALTY=0.289438 PRIMER_LEFT_1_SEQUENCE=TGGACTAGCCCAACCATAGC PRIMER_RIGHT_1_SEQUENCE=GCATCATGTTTCCATCCTCC PRIMER_LEFT_1=52,20 PRIMER_RIGHT_1=179,20 PRIMER_LEFT_1_TM=60.096 PRIMER_RIGHT_1_TM=60.289 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.1000 PRIMER_RIGHT_1_END_STABILITY=9.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=128 PRIMER_PAIR_2_PENALTY=0.385209 PRIMER_LEFT_2_PENALTY=0.095771 PRIMER_RIGHT_2_PENALTY=0.289438 PRIMER_LEFT_2_SEQUENCE=TGGACTAGCCCAACCATAGC PRIMER_RIGHT_2_SEQUENCE=GGCATCATGTTTCCATCCTC PRIMER_LEFT_2=52,20 PRIMER_RIGHT_2=180,20 PRIMER_LEFT_2_TM=60.096 PRIMER_RIGHT_2_TM=60.289 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.1000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=129 PRIMER_PAIR_3_PENALTY=0.448041 PRIMER_LEFT_3_PENALTY=0.375090 PRIMER_RIGHT_3_PENALTY=0.072951 PRIMER_LEFT_3_SEQUENCE=TGTTGGACTAGCCCAACCAT PRIMER_RIGHT_3_SEQUENCE=CATGTTTCCATCCTCCTGCT PRIMER_LEFT_3=49,20 PRIMER_RIGHT_3=175,20 PRIMER_LEFT_3_TM=60.375 PRIMER_RIGHT_3_TM=60.073 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=12.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=6.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.8000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=127 PRIMER_PAIR_4_PENALTY=0.618890 PRIMER_LEFT_4_PENALTY=0.375090 PRIMER_RIGHT_4_PENALTY=0.243800 PRIMER_LEFT_4_SEQUENCE=TGTTGGACTAGCCCAACCAT PRIMER_RIGHT_4_SEQUENCE=AAGGGCATCATGTTTCCATC PRIMER_LEFT_4=49,20 PRIMER_RIGHT_4=183,20 PRIMER_LEFT_4_TM=60.375 PRIMER_RIGHT_4_TM=59.756 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=12.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=6.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=7.8000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=135 = SEQUENCE_ID=16.RHAP131FF1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCCACGAATCAAAGAAAAGGCAAACTAGGGAAAGAAAAGCTAGTCTCAAACACCTGACCAATGGAAACCTTAGAAAGAGTGAGAGCCAAAATGATGCAGTTCCCAGTACTCCCTTATTGAAGATGATCTAGTTCCAAGCACTCACTACTCATAAATACAGTACCTGGTTCTCCCCTATCCAATGTAATACAGTACTTAGTACTCCCCCACCCAGTGATATAGTGCTTAGTACTCAGCCATGATGAGAGTACCTAACACTTCCCCCACCCAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGNGGGGGTGNNTNTGTNNGNNTCNGGGGGTNNNGCGCGCNNNNNNGNGNAGCATGGNGNNANNNGTCTNNTGATGTGAAANNNTATNNNCGCACAATNNCTGCANAACANANGAGNNGGNGNGNNAAATGNGGTGAAANCNGNGGNGCCNANTGAGNGAGNNNCACACANTAANANNGNGGNGCNNNNNGGCGCNNTGNANNNGGGGNANNNNNNGTGNNANCTGNACTGNNNGNNNGGCNTNNNGGNNGGNG SEQUENCE_INCLUDED_REGION=11,569 SEQUENCE_TARGET=283,40 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=16.RHAP131FF7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNCCCCTTAAATGCATCACGAGAATTCTCACTTGAGATGATTTCAATTCTAGCTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTGTTCCGGCGCGCACACACGCACATATACAGCAAAATATAACTGAATGCCAAAAAGCATTGTCATGTCCTGTGAAGGGCACAATTGTAATCACACAGGTATGAAAAGCTTAAAATCCTTCCATTACCTTCCATGCCTGAACACTGACAGGGTCGGGCTCAGGAGCAGAGCCCACTTCCTGAGCTATTTATTTGTATCTCTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=12,305 SEQUENCE_TARGET=66,44 P3_COMMENT=CL is CCCCTTAAATGCATCACGAG P3_COMMENT=CR is TGTATATGTGCGTGTGTGCG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.668814 PRIMER_LEFT_0_PENALTY=0.460175 PRIMER_RIGHT_0_PENALTY=0.208639 PRIMER_LEFT_0_SEQUENCE=CCCCTTAAATGCATCACGAG PRIMER_RIGHT_0_SEQUENCE=TGTATATGTGCGTGTGTGCG PRIMER_LEFT_0=12,20 PRIMER_RIGHT_0=142,20 PRIMER_LEFT_0_TM=60.460 PRIMER_RIGHT_0_TM=60.209 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.1000 PRIMER_RIGHT_0_END_STABILITY=9.9000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=131 PRIMER_PAIR_1_PENALTY=1.104173 PRIMER_LEFT_1_PENALTY=0.460175 PRIMER_RIGHT_1_PENALTY=0.643998 PRIMER_LEFT_1_SEQUENCE=CCCCTTAAATGCATCACGAG PRIMER_RIGHT_1_SEQUENCE=CATGACAATGCTTTTTGGCA PRIMER_LEFT_1=12,20 PRIMER_RIGHT_1=178,20 PRIMER_LEFT_1_TM=60.460 PRIMER_RIGHT_1_TM=60.644 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=40.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_END_STABILITY=8.1000 PRIMER_RIGHT_1_END_STABILITY=10.0000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=167 PRIMER_PAIR_2_PENALTY=1.159164 PRIMER_LEFT_2_PENALTY=0.460175 PRIMER_RIGHT_2_PENALTY=0.698990 PRIMER_LEFT_2_SEQUENCE=CCCCTTAAATGCATCACGAG PRIMER_RIGHT_2_SEQUENCE=CAATGCTTTTTGGCATTCAG PRIMER_LEFT_2=12,20 PRIMER_RIGHT_2=173,20 PRIMER_LEFT_2_TM=60.460 PRIMER_RIGHT_2_TM=59.301 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=162 PRIMER_PAIR_3_PENALTY=1.290037 PRIMER_LEFT_3_PENALTY=1.081398 PRIMER_RIGHT_3_PENALTY=0.208639 PRIMER_LEFT_3_SEQUENCE=CCCTTAAATGCATCACGAGAA PRIMER_RIGHT_3_SEQUENCE=TGTATATGTGCGTGTGTGCG PRIMER_LEFT_3=13,21 PRIMER_RIGHT_3=142,20 PRIMER_LEFT_3_TM=60.081 PRIMER_RIGHT_3_TM=60.209 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=9.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=130 PRIMER_PAIR_4_PENALTY=1.299052 PRIMER_LEFT_4_PENALTY=0.460175 PRIMER_RIGHT_4_PENALTY=0.838878 PRIMER_LEFT_4_SEQUENCE=CCCCTTAAATGCATCACGAG PRIMER_RIGHT_4_SEQUENCE=ACAATGCTTTTTGGCATTCA PRIMER_LEFT_4=12,20 PRIMER_RIGHT_4=174,20 PRIMER_LEFT_4_TM=60.460 PRIMER_RIGHT_4_TM=59.161 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=35.000 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=163 = SEQUENCE_ID=18.RHAP131FF9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNCCGGTTGCATGCATGTACCCGCCCAGGAGCTAAGTCTGATTTTCGGATAAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTAGCTCATTTGAAAACTAGCTTATTGGCTCTGAGGCTGGGCTCTTCTAAGCCTTCCACAGCTAGTGTGCCCTGAGCTTCACTCCTAGCTCAATCCCTCAGTTTAGTTGCCTAGTTACCTTCTGTGAAGCCCATTGGTCTTGTTACCCAAGATGCTTTGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=16,274 SEQUENCE_TARGET=69,58 P3_COMMENT=CL is GCCCAGGAGCTAAGTCTGATT P3_COMMENT=CR is CCAGCCTCAGAGCCAATAAG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.166815 PRIMER_LEFT_0_PENALTY=1.137562 PRIMER_RIGHT_0_PENALTY=0.029253 PRIMER_LEFT_0_SEQUENCE=GCCCAGGAGCTAAGTCTGATT PRIMER_RIGHT_0_SEQUENCE=CCAGCCTCAGAGCCAATAAG PRIMER_LEFT_0=36,21 PRIMER_RIGHT_0=167,20 PRIMER_LEFT_0_TM=59.862 PRIMER_RIGHT_0_TM=59.971 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=6.9000 PRIMER_RIGHT_0_END_STABILITY=5.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=132 PRIMER_PAIR_1_PENALTY=1.194449 PRIMER_LEFT_1_PENALTY=1.137562 PRIMER_RIGHT_1_PENALTY=0.056887 PRIMER_LEFT_1_SEQUENCE=GCCCAGGAGCTAAGTCTGATT PRIMER_RIGHT_1_SEQUENCE=AGTGAAGCTCAGGGCACACT PRIMER_LEFT_1=36,21 PRIMER_RIGHT_1=210,20 PRIMER_LEFT_1_TM=59.862 PRIMER_RIGHT_1_TM=60.057 PRIMER_LEFT_1_GC_PERCENT=52.381 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.1000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=175 PRIMER_PAIR_2_PENALTY=1.254144 PRIMER_LEFT_2_PENALTY=1.137562 PRIMER_RIGHT_2_PENALTY=0.116582 PRIMER_LEFT_2_SEQUENCE=GCCCAGGAGCTAAGTCTGATT PRIMER_RIGHT_2_SEQUENCE=AAGGCTTAGAAGAGCCCAGC PRIMER_LEFT_2=36,21 PRIMER_RIGHT_2=182,20 PRIMER_LEFT_2_TM=59.862 PRIMER_RIGHT_2_TM=60.117 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=9.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=147 PRIMER_PAIR_3_PENALTY=1.255379 PRIMER_LEFT_3_PENALTY=1.137562 PRIMER_RIGHT_3_PENALTY=0.117817 PRIMER_LEFT_3_SEQUENCE=GCCCAGGAGCTAAGTCTGATT PRIMER_RIGHT_3_SEQUENCE=CAGCCTCAGAGCCAATAAGC PRIMER_LEFT_3=36,21 PRIMER_RIGHT_3=166,20 PRIMER_LEFT_3_TM=59.862 PRIMER_RIGHT_3_TM=60.118 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=7.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=131 PRIMER_PAIR_4_PENALTY=1.301690 PRIMER_LEFT_4_PENALTY=1.137562 PRIMER_RIGHT_4_PENALTY=0.164128 PRIMER_LEFT_4_SEQUENCE=GCCCAGGAGCTAAGTCTGATT PRIMER_RIGHT_4_SEQUENCE=GAAGCTCAGGGCACACTAGC PRIMER_LEFT_4=36,21 PRIMER_RIGHT_4=207,20 PRIMER_LEFT_4_TM=59.862 PRIMER_RIGHT_4_TM=60.164 PRIMER_LEFT_4_GC_PERCENT=52.381 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=7.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=172 = SEQUENCE_ID=19.RHAP131FG1.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCCAGGATTGATTGACGCCTTGTGATGATGTCCTCTTCTAACCCTGCTCTTATACAAGGTGGAGTAGCTGGTATGTTTGAATGTTTAAATAAAAGGTACCCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTGCTTGGTATAGGTGCTTATAACTGTATCTCAGGTCCTCTGGGTTTGACAGTCAACATAACAGCCATGACAGCCCTGTCCACTAGGGCACTTCTATCAGTTCTGATATCACAGTGGAGCCAAACATGGACTGTTAGCATTCTATGCTCCACCCAGTGTTACCTAGCAACAACCAGGCTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,331 SEQUENCE_TARGET=113,46 P3_COMMENT=CL is CCCTGCTCTTATACAAGGTGG P3_COMMENT=CR is GTCAAACCCAGAGGACCTGA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.358596 PRIMER_LEFT_0_PENALTY=2.270426 PRIMER_RIGHT_0_PENALTY=0.088170 PRIMER_LEFT_0_SEQUENCE=CCCTGCTCTTATACAAGGTGG PRIMER_RIGHT_0_SEQUENCE=GTCAAACCCAGAGGACCTGA PRIMER_LEFT_0=51,21 PRIMER_RIGHT_0=209,20 PRIMER_LEFT_0_TM=58.730 PRIMER_RIGHT_0_TM=60.088 PRIMER_LEFT_0_GC_PERCENT=52.381 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=6.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=4.00 PRIMER_RIGHT_0_SELF_END=1.00 PRIMER_LEFT_0_END_STABILITY=9.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=159 PRIMER_PAIR_1_PENALTY=2.499607 PRIMER_LEFT_1_PENALTY=2.270426 PRIMER_RIGHT_1_PENALTY=0.229181 PRIMER_LEFT_1_SEQUENCE=CCCTGCTCTTATACAAGGTGG PRIMER_RIGHT_1_SEQUENCE=CAAACCCAGAGGACCTGAGA PRIMER_LEFT_1=51,21 PRIMER_RIGHT_1=207,20 PRIMER_LEFT_1_TM=58.730 PRIMER_RIGHT_1_TM=60.229 PRIMER_LEFT_1_GC_PERCENT=52.381 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=4.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=157 PRIMER_PAIR_2_PENALTY=2.499607 PRIMER_LEFT_2_PENALTY=2.270426 PRIMER_RIGHT_2_PENALTY=0.229181 PRIMER_LEFT_2_SEQUENCE=CCCTGCTCTTATACAAGGTGG PRIMER_RIGHT_2_SEQUENCE=TCAAACCCAGAGGACCTGAG PRIMER_LEFT_2=51,21 PRIMER_RIGHT_2=208,20 PRIMER_LEFT_2_TM=58.730 PRIMER_RIGHT_2_TM=60.229 PRIMER_LEFT_2_GC_PERCENT=52.381 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_END_STABILITY=9.4000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=158 PRIMER_PAIR_3_PENALTY=2.545053 PRIMER_LEFT_3_PENALTY=2.456883 PRIMER_RIGHT_3_PENALTY=0.088170 PRIMER_LEFT_3_SEQUENCE=ACCCTGCTCTTATACAAGGTGG PRIMER_RIGHT_3_SEQUENCE=GTCAAACCCAGAGGACCTGA PRIMER_LEFT_3=50,22 PRIMER_RIGHT_3=209,20 PRIMER_LEFT_3_TM=59.543 PRIMER_RIGHT_3_TM=60.088 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=160 PRIMER_PAIR_4_PENALTY=2.550792 PRIMER_LEFT_4_PENALTY=2.462622 PRIMER_RIGHT_4_PENALTY=0.088170 PRIMER_LEFT_4_SEQUENCE=TGTCCTCTTCTAACCCTGCTC PRIMER_RIGHT_4_SEQUENCE=GTCAAACCCAGAGGACCTGA PRIMER_LEFT_4=38,21 PRIMER_RIGHT_4=209,20 PRIMER_LEFT_4_TM=58.537 PRIMER_RIGHT_4_TM=60.088 PRIMER_LEFT_4_GC_PERCENT=52.381 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=7.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=172 = SEQUENCE_ID=19.RHAP131FG7.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCATGGATAACAGGGATGGACAACAAGGAAGAGCTACAGAGACATCCAGACAGCCATTATCTGCCTCATACTATCCCAACTGTTACACTTCCTATCCATACCTGCTGCTTTCAAAACCAAGTCTAGCCCCTTCTGAGAATTATTTAGGGGTGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGAGAACAACTTGCAGAAGAAGGCTCTTTCTTACCTTCCACCACATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,258 SEQUENCE_TARGET=171,52 P3_COMMENT=CL is CCAAGTCTAGCCCCTTCTGA P3_COMMENT=CR is CCATGTGGTGGAAGGTAAGAA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.737208 PRIMER_LEFT_0_PENALTY=0.575355 PRIMER_RIGHT_0_PENALTY=1.161853 PRIMER_LEFT_0_SEQUENCE=CCAAGTCTAGCCCCTTCTGA PRIMER_RIGHT_0_SEQUENCE=CCATGTGGTGGAAGGTAAGAA PRIMER_LEFT_0=130,20 PRIMER_RIGHT_0=271,21 PRIMER_LEFT_0_TM=59.425 PRIMER_RIGHT_0_TM=59.838 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=47.619 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.7000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=4.00 PRIMER_PAIR_0_PRODUCT_SIZE=142 PRIMER_PAIR_1_PENALTY=1.742019 PRIMER_LEFT_1_PENALTY=0.580167 PRIMER_RIGHT_1_PENALTY=1.161853 PRIMER_LEFT_1_SEQUENCE=TCCATACCTGCTGCTTTCAA PRIMER_RIGHT_1_SEQUENCE=CCATGTGGTGGAAGGTAAGAA PRIMER_LEFT_1=108,20 PRIMER_RIGHT_1=271,21 PRIMER_LEFT_1_TM=59.420 PRIMER_RIGHT_1_TM=59.838 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=47.619 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=164 PRIMER_PAIR_2_PENALTY=1.968469 PRIMER_LEFT_2_PENALTY=0.806616 PRIMER_RIGHT_2_PENALTY=1.161853 PRIMER_LEFT_2_SEQUENCE=ACCTGCTGCTTTCAAAACCA PRIMER_RIGHT_2_SEQUENCE=CCATGTGGTGGAAGGTAAGAA PRIMER_LEFT_2=113,20 PRIMER_RIGHT_2=271,21 PRIMER_LEFT_2_TM=60.807 PRIMER_RIGHT_2_TM=59.838 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=6.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=7.0000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=159 PRIMER_PAIR_3_PENALTY=1.991414 PRIMER_LEFT_3_PENALTY=0.829561 PRIMER_RIGHT_3_PENALTY=1.161853 PRIMER_LEFT_3_SEQUENCE=TCAAAACCAAGTCTAGCCCC PRIMER_RIGHT_3_SEQUENCE=CCATGTGGTGGAAGGTAAGAA PRIMER_LEFT_3=124,20 PRIMER_RIGHT_3=271,21 PRIMER_LEFT_3_TM=59.170 PRIMER_RIGHT_3_TM=59.838 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=12.4000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=148 PRIMER_PAIR_4_PENALTY=2.161329 PRIMER_LEFT_4_PENALTY=0.575355 PRIMER_RIGHT_4_PENALTY=1.585973 PRIMER_LEFT_4_SEQUENCE=CCAAGTCTAGCCCCTTCTGA PRIMER_RIGHT_4_SEQUENCE=CCATGTGGTGGAAGGTAAGA PRIMER_LEFT_4=130,20 PRIMER_RIGHT_4=271,20 PRIMER_LEFT_4_TM=59.425 PRIMER_RIGHT_4_TM=58.414 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PAIR_4_PRODUCT_SIZE=142 = SEQUENCE_ID=20.RHAP131FG2.seq SEQUENCE_TEMPLATE=CCGGGTTTTTGTGTATGCTTGGGTGTGGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATTTGTATTTGTGTTATANNNNNNNNNNNNNTTTGCATGTGCGTGTGTGAGTGTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,149 SEQUENCE_TARGET=31,60 SEQUENCE_TARGET=111,13 P3_COMMENT=CL is CCGGGTTTTTGTGTATGCTT P3_COMMENT=CR is GACACTCACACACGCACATG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.367549 PRIMER_LEFT_0_PENALTY=0.139129 PRIMER_RIGHT_0_PENALTY=0.228420 PRIMER_LEFT_0_SEQUENCE=CCGGGTTTTTGTGTATGCTT PRIMER_RIGHT_0_SEQUENCE=GACACTCACACACGCACATG PRIMER_LEFT_0=0,20 PRIMER_RIGHT_0=147,20 PRIMER_LEFT_0_TM=59.861 PRIMER_RIGHT_0_TM=59.772 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=6.6000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=148 PRIMER_PAIR_1_PENALTY=0.388170 PRIMER_LEFT_1_PENALTY=0.159750 PRIMER_RIGHT_1_PENALTY=0.228420 PRIMER_LEFT_1_SEQUENCE=TGTATGCTTGGGTGTGGGTA PRIMER_RIGHT_1_SEQUENCE=GACACTCACACACGCACATG PRIMER_LEFT_1=11,20 PRIMER_RIGHT_1=147,20 PRIMER_LEFT_1_TM=59.840 PRIMER_RIGHT_1_TM=59.772 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=2.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.4000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=7.00 PRIMER_PAIR_1_COMPL_END=4.00 PRIMER_PAIR_1_PRODUCT_SIZE=137 PRIMER_PAIR_2_PENALTY=0.655200 PRIMER_LEFT_2_PENALTY=0.426779 PRIMER_RIGHT_2_PENALTY=0.228420 PRIMER_LEFT_2_SEQUENCE=TTGTGTATGCTTGGGTGTGG PRIMER_RIGHT_2_SEQUENCE=GACACTCACACACGCACATG PRIMER_LEFT_2=8,20 PRIMER_RIGHT_2=147,20 PRIMER_LEFT_2_TM=60.427 PRIMER_RIGHT_2_TM=59.772 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=7.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PAIR_2_PRODUCT_SIZE=140 PRIMER_PAIR_3_PENALTY=0.928284 PRIMER_LEFT_3_PENALTY=0.699863 PRIMER_RIGHT_3_PENALTY=0.228420 PRIMER_LEFT_3_SEQUENCE=GTGTATGCTTGGGTGTGGGT PRIMER_RIGHT_3_SEQUENCE=GACACTCACACACGCACATG PRIMER_LEFT_3=10,20 PRIMER_RIGHT_3=147,20 PRIMER_LEFT_3_TM=60.700 PRIMER_RIGHT_3_TM=59.772 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=4.00 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=7.00 PRIMER_PAIR_3_COMPL_END=5.00 PRIMER_PAIR_3_PRODUCT_SIZE=138 PRIMER_PAIR_4_PENALTY=1.144955 PRIMER_LEFT_4_PENALTY=0.916535 PRIMER_RIGHT_4_PENALTY=0.228420 PRIMER_LEFT_4_SEQUENCE=CGGGTTTTTGTGTATGCTTG PRIMER_RIGHT_4_SEQUENCE=GACACTCACACACGCACATG PRIMER_LEFT_4=1,20 PRIMER_RIGHT_4=147,20 PRIMER_LEFT_4_TM=59.083 PRIMER_RIGHT_4_TM=59.772 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=2.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=147 = SEQUENCE_ID=20.RHAP131FG8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCCGTGGTGGTTCTATTTGCAGCCCTAGCATCTTCCCAGAAGAGGCGTTGAAATGAAGGGGCTACTGAGCTGGCTCAGCAGGAAAAGGCACTTAATATACAACCTGGCAATGCAAAATTCAATCCTAGGAACTCACGTGAGAGGTGGAAGGAGAGAACCAACTCCACAAACTAGTCCTCCAGCCTCCACACATGTGCCCCACCCCCAACTCACATATATAACTACGTCATTAACGTTTTTTTAAATTGTCTTAAGTGACAGCTGTGTGTCTTGACACACTCCTGTAATCCTGGCATGAGAAGCAGGAGTTCGAGGCCACAGCAGCTGACTTTGTCCATCTGCAGACCTTTTAACTCTTCAAGCTGAAGGTGTGGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGCACCTTTCCTTGTGGATTTGGATTTGGAGTTTCCACCTGTCTTTAGTTAATACATTCCCATTGACCATTCTTTTTTAACATTGGTTGTACCAGAGACAACGGGTAGACATCATTTCCTGTGCTGCAAATAGGNGGAACTCCTAGAGCTTTATTGNCTCTGGNNTGTGNANAGNNTGNACTNGAGAACACAAAGNATGTCAATTTNTAGAATNTGACAATTTTAGNGTCNAACATGGGTTGTGT SEQUENCE_INCLUDED_REGION=15,666 SEQUENCE_TARGET=391,44 P3_COMMENT=CL is GGCATGAGAAGCAGGAGTTC P3_COMMENT=CR is ATCCACAAGGAAAGGTGCAC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.069264 PRIMER_LEFT_0_PENALTY=0.042732 PRIMER_RIGHT_0_PENALTY=0.026532 PRIMER_LEFT_0_SEQUENCE=GGCATGAGAAGCAGGAGTTC PRIMER_RIGHT_0_SEQUENCE=ATCCACAAGGAAAGGTGCAC PRIMER_LEFT_0=305,20 PRIMER_RIGHT_0=454,20 PRIMER_LEFT_0_TM=59.957 PRIMER_RIGHT_0_TM=59.973 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=6.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=8.2000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=150 PRIMER_PAIR_1_PENALTY=0.139587 PRIMER_LEFT_1_PENALTY=0.042732 PRIMER_RIGHT_1_PENALTY=0.096855 PRIMER_LEFT_1_SEQUENCE=GGCATGAGAAGCAGGAGTTC PRIMER_RIGHT_1_SEQUENCE=TCCAAATCCACAAGGAAAGG PRIMER_LEFT_1=305,20 PRIMER_RIGHT_1=459,20 PRIMER_LEFT_1_TM=59.957 PRIMER_RIGHT_1_TM=59.903 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=155 PRIMER_PAIR_2_PENALTY=0.170536 PRIMER_LEFT_2_PENALTY=0.008461 PRIMER_RIGHT_2_PENALTY=0.162074 PRIMER_LEFT_2_SEQUENCE=GCTGACTTTGTCCATCTGCA PRIMER_RIGHT_2_SEQUENCE=CCAAATCCAAATCCACAAGG PRIMER_LEFT_2=337,20 PRIMER_RIGHT_2=464,20 PRIMER_LEFT_2_TM=59.992 PRIMER_RIGHT_2_TM=60.162 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=8.5000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=5.00 PRIMER_PAIR_2_PRODUCT_SIZE=128 PRIMER_PAIR_3_PENALTY=0.204806 PRIMER_LEFT_3_PENALTY=0.042732 PRIMER_RIGHT_3_PENALTY=0.162074 PRIMER_LEFT_3_SEQUENCE=GGCATGAGAAGCAGGAGTTC PRIMER_RIGHT_3_SEQUENCE=CCAAATCCAAATCCACAAGG PRIMER_LEFT_3=305,20 PRIMER_RIGHT_3=464,20 PRIMER_LEFT_3_TM=59.957 PRIMER_RIGHT_3_TM=60.162 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=160 PRIMER_PAIR_4_PENALTY=0.241297 PRIMER_LEFT_4_PENALTY=0.008461 PRIMER_RIGHT_4_PENALTY=0.232835 PRIMER_LEFT_4_SEQUENCE=GCTGACTTTGTCCATCTGCA PRIMER_RIGHT_4_SEQUENCE=GGTGGAAACTCCAAATCCAA PRIMER_LEFT_4=337,20 PRIMER_RIGHT_4=474,20 PRIMER_LEFT_4_TM=59.992 PRIMER_RIGHT_4_TM=59.767 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=4.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=138 = SEQUENCE_ID=23.RHAP131FH2.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCTCACTTCTGCTACACAGTTAGCTTCCTTTTCAAAACCTCGTCTTCCTACTTTGGATCCCTTTCTCACCTCCTCTTCTGCTTCCCTTTTTGTTATGGTGGATTGTTCTTGGCTGTCTGTCTGCTTTATTCTTCTTGATCCCCACACCCCTTATGAGTAGCCATGAGATCTCCAGATGTCCGAGGCTTCTGTTGCTCTTTGGAGTTGACTGACACATGCCTGAACCACAGGGTTCACTGATAGCTCTCTGTGGTCCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCATGTATGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,322 SEQUENCE_TARGET=281,46 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=23.RHAP131FH8.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCAGCATTTCCCAAGCTGGAGGAGCTANACAGACAGTAAGTAGTTTTTGGCTAATTAATTATCTTCCATAGAAAACAATGTTTCTCACAATTCGTCCTCTTTGTTAATATATTTTCAGAATGCCCCCCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTCAGCCAGCATGCACTGCGTTTCTGAAATGTCTACTAATAGTAAAGTATGGCTATGATAAGTGACTGGTGACTGATGAGAGAGGTTTCCAGAGCTTCTGGACAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=15,285 SEQUENCE_TARGET=144,46 P3_COMMENT=CL is ATATTTTCAGAATGCCCCCC P3_COMMENT=CR is TCCAGAAGCTCTGGAAACCT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.001046 PRIMER_LEFT_0_PENALTY=0.015502 PRIMER_RIGHT_0_PENALTY=0.985544 PRIMER_LEFT_0_SEQUENCE=ATATTTTCAGAATGCCCCCC PRIMER_RIGHT_0_SEQUENCE=CCAGAAGCTCTGGAAACCTC PRIMER_LEFT_0=122,20 PRIMER_RIGHT_0=293,20 PRIMER_LEFT_0_TM=59.984 PRIMER_RIGHT_0_TM=59.014 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=12.4000 PRIMER_RIGHT_0_END_STABILITY=7.6000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=172 PRIMER_PAIR_1_PENALTY=1.001046 PRIMER_LEFT_1_PENALTY=0.015502 PRIMER_RIGHT_1_PENALTY=0.985544 PRIMER_LEFT_1_SEQUENCE=ATATTTTCAGAATGCCCCCC PRIMER_RIGHT_1_SEQUENCE=TCCAGAAGCTCTGGAAACCT PRIMER_LEFT_1=122,20 PRIMER_RIGHT_1=294,20 PRIMER_LEFT_1_TM=59.984 PRIMER_RIGHT_1_TM=59.014 PRIMER_LEFT_1_GC_PERCENT=45.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=10.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=12.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=173 PRIMER_PAIR_2_PENALTY=1.017442 PRIMER_LEFT_2_PENALTY=0.015502 PRIMER_RIGHT_2_PENALTY=1.001941 PRIMER_LEFT_2_SEQUENCE=ATATTTTCAGAATGCCCCCC PRIMER_RIGHT_2_SEQUENCE=CCAGAAGCTCTGGAAACCTCT PRIMER_LEFT_2=122,20 PRIMER_RIGHT_2=293,21 PRIMER_LEFT_2_TM=59.984 PRIMER_RIGHT_2_TM=60.002 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=52.381 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=12.4000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=172 PRIMER_PAIR_3_PENALTY=1.145086 PRIMER_LEFT_3_PENALTY=0.015502 PRIMER_RIGHT_3_PENALTY=1.129584 PRIMER_LEFT_3_SEQUENCE=ATATTTTCAGAATGCCCCCC PRIMER_RIGHT_3_SEQUENCE=GTCCAGAAGCTCTGGAAACCT PRIMER_LEFT_3=122,20 PRIMER_RIGHT_3=295,21 PRIMER_LEFT_3_TM=59.984 PRIMER_RIGHT_3_TM=59.870 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=52.381 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=10.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=12.4000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=174 PRIMER_PAIR_4_PENALTY=1.147207 PRIMER_LEFT_4_PENALTY=0.015502 PRIMER_RIGHT_4_PENALTY=1.131705 PRIMER_LEFT_4_SEQUENCE=ATATTTTCAGAATGCCCCCC PRIMER_RIGHT_4_SEQUENCE=GTCCAGAAGCTCTGGAAACC PRIMER_LEFT_4=122,20 PRIMER_RIGHT_4=295,20 PRIMER_LEFT_4_TM=59.984 PRIMER_RIGHT_4_TM=58.868 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=10.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=12.4000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=174 = SEQUENCE_ID=24.RHAP131FH9.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNCTCATTCTCCATCAAATGCCACGAACTTCAATGGCTAACCCTTGGGTTCCGTGTCTATGAGGAAGTATACACAATGCCAATGAGCCAGAGATATTCTGTAACCTTCTATTTCACAGTTCCTGTGATCAGCTCCTTAAAGTGGCAGAGGACATAGGAGTTGGCTTTCTCTAGTCATGTACAGTCTTATAAAAATCATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGCAAGAGGAAAAATAGATTTAGTAAAAGCAAAAAAAATTGGTAATGGAGAAACAAAAATAATATTGAATCAAGACCTGAGCATTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=15,337 SEQUENCE_TARGET=214,50 P3_COMMENT=CL is GCTCCTTAAAGTGGCAGAGG P3_COMMENT=CR is TCTCCATTACCAATTTTTTTTGC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.515792 PRIMER_LEFT_0_PENALTY=0.923358 PRIMER_RIGHT_0_PENALTY=3.592434 PRIMER_LEFT_0_SEQUENCE=GCTCCTTAAAGTGGCAGAGG PRIMER_RIGHT_0_SEQUENCE=TCTCCATTACCAATTTTTTTTGC PRIMER_LEFT_0=143,20 PRIMER_RIGHT_0=315,23 PRIMER_LEFT_0_TM=59.077 PRIMER_RIGHT_0_TM=59.408 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=30.435 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.9000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=173 PRIMER_PAIR_1_PENALTY=4.618441 PRIMER_LEFT_1_PENALTY=1.026007 PRIMER_RIGHT_1_PENALTY=3.592434 PRIMER_LEFT_1_SEQUENCE=AGCTCCTTAAAGTGGCAGAGG PRIMER_RIGHT_1_SEQUENCE=TCTCCATTACCAATTTTTTTTGC PRIMER_LEFT_1=142,21 PRIMER_RIGHT_1=315,23 PRIMER_LEFT_1_TM=60.026 PRIMER_RIGHT_1_TM=59.408 PRIMER_LEFT_1_GC_PERCENT=52.381 PRIMER_RIGHT_1_GC_PERCENT=30.435 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=8.8000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=174 PRIMER_PAIR_2_PENALTY=4.731626 PRIMER_LEFT_2_PENALTY=1.139192 PRIMER_RIGHT_2_PENALTY=3.592434 PRIMER_LEFT_2_SEQUENCE=TCCTTAAAGTGGCAGAGGACA PRIMER_RIGHT_2_SEQUENCE=TCTCCATTACCAATTTTTTTTGC PRIMER_LEFT_2=145,21 PRIMER_RIGHT_2=315,23 PRIMER_LEFT_2_TM=59.861 PRIMER_RIGHT_2_TM=59.408 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=30.435 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=8.8000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=171 PRIMER_PAIR_3_PENALTY=4.854048 PRIMER_LEFT_3_PENALTY=1.261614 PRIMER_RIGHT_3_PENALTY=3.592434 PRIMER_LEFT_3_SEQUENCE=TGGCAGAGGACATAGGAGTTG PRIMER_RIGHT_3_SEQUENCE=TCTCCATTACCAATTTTTTTTGC PRIMER_LEFT_3=154,21 PRIMER_RIGHT_3=315,23 PRIMER_LEFT_3_TM=60.262 PRIMER_RIGHT_3_TM=59.408 PRIMER_LEFT_3_GC_PERCENT=52.381 PRIMER_RIGHT_3_GC_PERCENT=30.435 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=8.8000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PAIR_3_PRODUCT_SIZE=162 PRIMER_PAIR_4_PENALTY=4.934726 PRIMER_LEFT_4_PENALTY=0.923358 PRIMER_RIGHT_4_PENALTY=4.011367 PRIMER_LEFT_4_SEQUENCE=GCTCCTTAAAGTGGCAGAGG PRIMER_RIGHT_4_SEQUENCE=TCCATTACCAATTTTTTTTGCTT PRIMER_LEFT_4=143,20 PRIMER_RIGHT_4=313,23 PRIMER_LEFT_4_TM=59.077 PRIMER_RIGHT_4_TM=58.989 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=26.087 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=8.5000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=171 = SEQUENCE_ID=25.RHAP131FA10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCCAGGAAGATATTTTATACATAAAATATTGAATATGTTTTACCACAATGGGGCATAGCACAATGTAAAGCATTTCACTATCATAATAGAGAGTTTTTGATTCTAGAAATGTGCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACAGAGCCCACAGGGAGAGNNNNNNNNNNNNNNAGAGAGAAATACAAAATGTAATTATCTTTAATTCTAAGAACCCTGCTATGATGGTTAGTTTTTATTGCCAACTTGGATGGGTTTAGAACCAGCCTGGCAACAAAACTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=27,304 SEQUENCE_TARGET=143,42 SEQUENCE_TARGET=206,14 P3_COMMENT=CL is TGGGGCATAGCACAATGTAA P3_COMMENT=CR is CTCTCCCTGTGGGCTCTGT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.451089 PRIMER_LEFT_0_PENALTY=0.046801 PRIMER_RIGHT_0_PENALTY=1.404288 PRIMER_LEFT_0_SEQUENCE=TGGGGCATAGCACAATGTAA PRIMER_RIGHT_0_SEQUENCE=CTCTCCCTGTGGGCTCTGT PRIMER_LEFT_0=75,20 PRIMER_RIGHT_0=205,19 PRIMER_LEFT_0_TM=59.953 PRIMER_RIGHT_0_TM=60.404 PRIMER_LEFT_0_GC_PERCENT=45.000 PRIMER_RIGHT_0_GC_PERCENT=63.158 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=3.00 PRIMER_LEFT_0_SELF_END=1.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=6.0000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=131 PRIMER_PAIR_1_PENALTY=1.471724 PRIMER_LEFT_1_PENALTY=1.404288 PRIMER_RIGHT_1_PENALTY=0.067436 PRIMER_LEFT_1_SEQUENCE=ACAGAGCCCACAGGGAGAG PRIMER_RIGHT_1_SEQUENCE=GCTGGTTCTAAACCCATCCA PRIMER_LEFT_1=187,19 PRIMER_RIGHT_1=312,20 PRIMER_LEFT_1_TM=60.404 PRIMER_RIGHT_1_TM=59.933 PRIMER_LEFT_1_GC_PERCENT=63.158 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=126 PRIMER_PAIR_2_PENALTY=1.625656 PRIMER_LEFT_2_PENALTY=0.221368 PRIMER_RIGHT_2_PENALTY=1.404288 PRIMER_LEFT_2_SEQUENCE=AATGGGGCATAGCACAATGT PRIMER_RIGHT_2_SEQUENCE=CTCTCCCTGTGGGCTCTGT PRIMER_LEFT_2=73,20 PRIMER_RIGHT_2=205,19 PRIMER_LEFT_2_TM=60.221 PRIMER_RIGHT_2_TM=60.404 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=63.158 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=133 PRIMER_PAIR_3_PENALTY=1.625656 PRIMER_LEFT_3_PENALTY=0.221368 PRIMER_RIGHT_3_PENALTY=1.404288 PRIMER_LEFT_3_SEQUENCE=ACAATGGGGCATAGCACAAT PRIMER_RIGHT_3_SEQUENCE=CTCTCCCTGTGGGCTCTGT PRIMER_LEFT_3=71,20 PRIMER_RIGHT_3=205,19 PRIMER_LEFT_3_TM=60.221 PRIMER_RIGHT_3_TM=60.404 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=63.158 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=135 PRIMER_PAIR_4_PENALTY=1.653189 PRIMER_LEFT_4_PENALTY=1.404288 PRIMER_RIGHT_4_PENALTY=0.248900 PRIMER_LEFT_4_SEQUENCE=ACAGAGCCCACAGGGAGAG PRIMER_RIGHT_4_SEQUENCE=TTGCCAGGCTGGTTCTAAAC PRIMER_LEFT_4=187,19 PRIMER_RIGHT_4=319,20 PRIMER_LEFT_4_TM=60.404 PRIMER_RIGHT_4_TM=60.249 PRIMER_LEFT_4_GC_PERCENT=63.158 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.0000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=133 = SEQUENCE_ID=26.RHAP131FA11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCCTACTACAACAAATCAATCTAAAAATAGACCTTTTATTAGCTCACAATTTTAAGGATTTTCTTTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=15,130 SEQUENCE_TARGET=15,57 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=26.RHAP131FA5.seq SEQUENCE_TEMPLATE=CCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCCTCATTGTTGTTTTGATTTCAGGTTTATGCTAACTGAATTTTGTGTTACCTTGACTGGGTCATGAGACCATGCTATTCCTTTGAACCTTTTTCTGACTTTTGTGAGGACACTTTCTGTTGAGCTCAATATAGACTGATGAAAGTAGATTTTCCTCCCTGACAGAAGCCCATCTAACCAGCCTGAGCAAAATGATTAGTCTCCTTTCAGGTGGAAGCCAACTCCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=0,277 SEQUENCE_TARGET=3,42 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=27.RHAP131FA12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCGACAAAGATGACATGGTTTCTANTGGAGGCAGGGCTGAGATGAGGAGTCACATTCTCTATTGGCAGGCATTTCCCCCTGTCTTGTTATTTTCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNNNNNNNNNNNNNNTGTNNCNNNNNGNGNNNNNCNCNACACAACGNNTGTGCGTGAAAANTGAAGTTTTTTGTGTGTCAGTGAGACAGTGTCTTCNTGGGTGCCCGAGGCAANCNAGGGNATCCACCCGAGCGTTAGCCTCCAGANATTATTAACAANGGCCAAAACATCATNCCTACCTAAGTGAGAAAAAGCAATTTNGANAAAAANCGNGCTCCNAATCCAAAACNTGGGCATGGGCTAGATCCNNAGGTGAAAATNGTAGGCGGTCNAGATTCCNAACAACNAANNANCCGGAAACATGNTGTGTGATAACCANAGGGTGACNNNNGAGTNANNNACACAAAATAANTGCGGNGCGCACAAAGNGCGNNNTCCAGNGGGGAAACCTGNGNGNGCCAACTTNNATAAANNNGNNGGGAANNANCGGGNGNGTTGCGAGGTTGNGNAANNNNNCCNCGGNAGNNTNGTANNNNCNCGNNNANGNGGGGGGAGNGNGNTTGCNNNNNGGGNNNCCTGNNNANNNTTNNNANAGTGNAANGGGGANNNCCAANNNNGNAA SEQUENCE_INCLUDED_REGION=14,687 SEQUENCE_TARGET=141,14 SEQUENCE_TARGET=111,28 P3_COMMENT=CL is CTCTATTGGCAGGCATTTCC P3_COMMENT=CR is AGACACTGTCTCACTGACACACAA PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.420604 PRIMER_LEFT_0_PENALTY=0.332028 PRIMER_RIGHT_0_PENALTY=4.088575 PRIMER_LEFT_0_SEQUENCE=CTCTATTGGCAGGCATTTCC PRIMER_RIGHT_0_SEQUENCE=AGACACTGTCTCACTGACACACAA PRIMER_LEFT_0=70,20 PRIMER_RIGHT_0=233,24 PRIMER_LEFT_0_TM=59.668 PRIMER_RIGHT_0_TM=59.911 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.833 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=9.00 PRIMER_LEFT_0_SELF_END=3.00 PRIMER_RIGHT_0_SELF_END=3.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=7.0000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=164 PRIMER_PAIR_1_PENALTY=4.420604 PRIMER_LEFT_1_PENALTY=0.332028 PRIMER_RIGHT_1_PENALTY=4.088575 PRIMER_LEFT_1_SEQUENCE=CTCTATTGGCAGGCATTTCC PRIMER_RIGHT_1_SEQUENCE=AAGACACTGTCTCACTGACACACA PRIMER_LEFT_1=70,20 PRIMER_RIGHT_1=234,24 PRIMER_LEFT_1_TM=59.668 PRIMER_RIGHT_1_TM=59.911 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.833 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=9.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=165 PRIMER_PAIR_2_PENALTY=4.438083 PRIMER_LEFT_2_PENALTY=0.332028 PRIMER_RIGHT_2_PENALTY=4.106055 PRIMER_LEFT_2_SEQUENCE=CTCTATTGGCAGGCATTTCC PRIMER_RIGHT_2_SEQUENCE=CACTGTCTCACTGACACACAAAA PRIMER_LEFT_2=70,20 PRIMER_RIGHT_2=230,23 PRIMER_LEFT_2_TM=59.668 PRIMER_RIGHT_2_TM=58.894 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=9.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=8.5000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PAIR_2_PRODUCT_SIZE=161 PRIMER_PAIR_3_PENALTY=4.481654 PRIMER_LEFT_3_PENALTY=0.332028 PRIMER_RIGHT_3_PENALTY=4.149625 PRIMER_LEFT_3_SEQUENCE=CTCTATTGGCAGGCATTTCC PRIMER_RIGHT_3_SEQUENCE=GACACTGTCTCACTGACACACAA PRIMER_LEFT_3=70,20 PRIMER_RIGHT_3=232,23 PRIMER_LEFT_3_TM=59.668 PRIMER_RIGHT_3_TM=58.850 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=47.826 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=9.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_END_STABILITY=8.5000 PRIMER_RIGHT_3_END_STABILITY=7.0000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=163 PRIMER_PAIR_4_PENALTY=4.556370 PRIMER_LEFT_4_PENALTY=0.332028 PRIMER_RIGHT_4_PENALTY=4.224341 PRIMER_LEFT_4_SEQUENCE=CTCTATTGGCAGGCATTTCC PRIMER_RIGHT_4_SEQUENCE=ACACTGTCTCACTGACACACAAAA PRIMER_LEFT_4=70,20 PRIMER_RIGHT_4=231,24 PRIMER_LEFT_4_TM=59.668 PRIMER_RIGHT_4_TM=59.776 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=9.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.5000 PRIMER_RIGHT_4_END_STABILITY=7.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=162 = SEQUENCE_ID=29.RHAP131FB5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCTTGGTGCATGCAGCCACATTTCCTCAAGGAGCATAGGGCTTATACAGTTCTTTCAGCACAAGTTTTGGGTCTTTTGTTCCACATTCACATGTCAGACTTGCTAGCCGGTTTTTTGTATATGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATATGTATTTGTGTTATATGTGTGTGTGTGTTTGCAAGTGCGTGGTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTTGTATTCGTGTTATTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTGTGTGTGTGCTATTTCTATNNGTTTTTATNTGTANNNNNNNNNNNNNNNNATNTGCGTGTGTGTGTGCNNNNNNNNNNNNNNNNNNNNNGTGTNNTCCNCNCCNGTATTTCACACNNAANACGTNNANNGNGAGAATTCCTNAAAATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,477 SEQUENCE_TARGET=148,44 SEQUENCE_TARGET=243,35 SEQUENCE_TARGET=301,40 SEQUENCE_TARGET=382,16 SEQUENCE_TARGET=416,18 P3_COMMENT=CL is CATGTCAGACTTGCTAGCCG P3_COMMENT=CR is ACGCACTTGCAAACACACAC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.218921 PRIMER_LEFT_0_PENALTY=0.384829 PRIMER_RIGHT_0_PENALTY=0.834091 PRIMER_LEFT_0_SEQUENCE=CATGTCAGACTTGCTAGCCG PRIMER_RIGHT_0_SEQUENCE=ACGCACTTGCAAACACACAC PRIMER_LEFT_0=112,20 PRIMER_RIGHT_0=236,20 PRIMER_LEFT_0_TM=59.615 PRIMER_RIGHT_0_TM=60.834 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=11.4000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=125 PRIMER_PAIR_1_PENALTY=1.456124 PRIMER_LEFT_1_PENALTY=0.384829 PRIMER_RIGHT_1_PENALTY=1.071294 PRIMER_LEFT_1_SEQUENCE=CATGTCAGACTTGCTAGCCG PRIMER_RIGHT_1_SEQUENCE=ACGCACTTGCAAACACACA PRIMER_LEFT_1=112,20 PRIMER_RIGHT_1=236,19 PRIMER_LEFT_1_TM=59.615 PRIMER_RIGHT_1_TM=59.929 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=47.368 PRIMER_LEFT_1_SELF_ANY=7.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=11.4000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PAIR_1_PRODUCT_SIZE=125 PRIMER_PAIR_2_PENALTY=1.456124 PRIMER_LEFT_2_PENALTY=0.384829 PRIMER_RIGHT_2_PENALTY=1.071294 PRIMER_LEFT_2_SEQUENCE=CATGTCAGACTTGCTAGCCG PRIMER_RIGHT_2_SEQUENCE=CACGCACTTGCAAACACAC PRIMER_LEFT_2=112,20 PRIMER_RIGHT_2=237,19 PRIMER_LEFT_2_TM=59.615 PRIMER_RIGHT_2_TM=59.929 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=52.632 PRIMER_LEFT_2_SELF_ANY=7.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=11.4000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=126 PRIMER_PAIR_3_PENALTY=1.489980 PRIMER_LEFT_3_PENALTY=0.418685 PRIMER_RIGHT_3_PENALTY=1.071294 PRIMER_LEFT_3_SEQUENCE=ATGTCAGACTTGCTAGCCGG PRIMER_RIGHT_3_SEQUENCE=CACGCACTTGCAAACACAC PRIMER_LEFT_3=113,20 PRIMER_RIGHT_3=237,19 PRIMER_LEFT_3_TM=60.419 PRIMER_RIGHT_3_TM=59.929 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=52.632 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=12.9000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=125 PRIMER_PAIR_4_PENALTY=1.838763 PRIMER_LEFT_4_PENALTY=1.004672 PRIMER_RIGHT_4_PENALTY=0.834091 PRIMER_LEFT_4_SEQUENCE=TTTGGGTCTTTTGTTCCACA PRIMER_RIGHT_4_SEQUENCE=ACGCACTTGCAAACACACAC PRIMER_LEFT_4=88,20 PRIMER_RIGHT_4=236,20 PRIMER_LEFT_4_TM=58.995 PRIMER_RIGHT_4_TM=60.834 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=149 = SEQUENCE_ID=30.RHAP131FB12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCGGACAGCTTTCTCCCTAGCTTTTCATTTCTGACAATAGTGCTCTGCCTTGATTCTAGAATGATTTCATGGAGGGAAGTTTGTCCTTTTTAGAGCTATCACAGCTTCACAGTCCTACCCACTTCACTTGTTTCTATGCCAATTCCCTAATACTTGGAGCAGTAATCACACAGCAATACCACAAGGGTGCTTACCCACAGTGCTTTCCTCAGTCAGGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGGTGCTTACCCACAGTGCTTTCCTTAGTCAGGAGCGTGTGTGTGTGTGGGGGGGGGGGGAGGCCAAGATGATAGCCCCTGGGATGGCACAGGTTACTTCCTTGGAGGACCTAACACCAAGACCAATGTGACCATTGCAGCCCACATCTGCACCATCTTTAGAACAACATCCTTTAGGGATACCCTCAAGAAAACACCAGTGATCAGTCTGTGATAGGCAGAGGACAGGTAGGGCNCNTCAAGCCCTTGATCCNCGTGTCNNTGACCACGCCTGGTGATGGGGATCTCNCGTCTTGTTTACCCCATGAGATGGCAGACCCACATGGGGGACGAGCACGNATCNGAAACATGGCAGAGNTGTTCCTGTGTAAANTGGTANCGGCACACTC SEQUENCE_INCLUDED_REGION=14,666 SEQUENCE_TARGET=234,54 P3_COMMENT=CL is GCAATACCACAAGGGTGCTT P3_COMMENT=CR is GGAAAGCACTGTGGGTAAGC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.259740 PRIMER_LEFT_0_PENALTY=0.000037 PRIMER_RIGHT_0_PENALTY=0.259704 PRIMER_LEFT_0_SEQUENCE=GCAATACCACAAGGGTGCTT PRIMER_RIGHT_0_SEQUENCE=GGAAAGCACTGTGGGTAAGC PRIMER_LEFT_0=186,20 PRIMER_RIGHT_0=314,20 PRIMER_LEFT_0_TM=60.000 PRIMER_RIGHT_0_TM=59.740 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=55.000 PRIMER_LEFT_0_SELF_ANY=3.00 PRIMER_RIGHT_0_SELF_ANY=5.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.5000 PRIMER_RIGHT_0_END_STABILITY=7.5000 PRIMER_PAIR_0_COMPL_ANY=6.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=129 PRIMER_PAIR_1_PENALTY=0.509682 PRIMER_LEFT_1_PENALTY=0.259704 PRIMER_RIGHT_1_PENALTY=0.249979 PRIMER_LEFT_1_SEQUENCE=GCTTACCCACAGTGCTTTCC PRIMER_RIGHT_1_SEQUENCE=ATCCCAGGGGCTATCATCTT PRIMER_LEFT_1=202,20 PRIMER_RIGHT_1=375,20 PRIMER_LEFT_1_TM=59.740 PRIMER_RIGHT_1_TM=59.750 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.5000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=174 PRIMER_PAIR_2_PENALTY=0.547997 PRIMER_LEFT_2_PENALTY=0.298018 PRIMER_RIGHT_2_PENALTY=0.249979 PRIMER_LEFT_2_SEQUENCE=CCCACAGTGCTTTCCTCAGT PRIMER_RIGHT_2_SEQUENCE=ATCCCAGGGGCTATCATCTT PRIMER_LEFT_2=207,20 PRIMER_RIGHT_2=375,20 PRIMER_LEFT_2_TM=60.298 PRIMER_RIGHT_2_TM=59.750 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.6000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=169 PRIMER_PAIR_3_PENALTY=0.547997 PRIMER_LEFT_3_PENALTY=0.298018 PRIMER_RIGHT_3_PENALTY=0.249979 PRIMER_LEFT_3_SEQUENCE=ACCCACAGTGCTTTCCTCAG PRIMER_RIGHT_3_SEQUENCE=ATCCCAGGGGCTATCATCTT PRIMER_LEFT_3=206,20 PRIMER_RIGHT_3=375,20 PRIMER_LEFT_3_TM=60.298 PRIMER_RIGHT_3_TM=59.750 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=170 PRIMER_PAIR_4_PENALTY=0.557677 PRIMER_LEFT_4_PENALTY=0.378958 PRIMER_RIGHT_4_PENALTY=0.178718 PRIMER_LEFT_4_SEQUENCE=AGCAATACCACAAGGGTGCT PRIMER_RIGHT_4_SEQUENCE=AAGCACTGTGGGTAAGCACC PRIMER_LEFT_4=185,20 PRIMER_RIGHT_4=311,20 PRIMER_LEFT_4_TM=59.621 PRIMER_RIGHT_4_TM=60.179 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=9.4000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=6.00 PRIMER_PAIR_4_PRODUCT_SIZE=127 = SEQUENCE_ID=31.RHAP131FC4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNCAAATGTGAGCAGATACTCTACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAATGTCATGATAAGGACCACTTTTGTACACTAACAAAAAACTGGATTTAAGCAATATATTTTAAAAACCTCAGCTATATAATAACAAAATAAAAGGGATAATAGAGCTATATGACATCTTACACACATATGCACACCATAGATGTTGTGTAGAGAAAGATGACACTGAGTTCCTGACCCCCAGCTCCTCCCACTGAGTACTATAATCACAGGTGTGTACCAGCAGTCCAGTTTCATTGCNTTAAGAATTAAAGCCAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=24,327 SEQUENCE_TARGET=48,42 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=32.RHAP131FC5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNCCCTGTGTTTCATTCTGAGTGCTACAGAGAAGCATTTTTGACAAGTTTAAGCTCTTACTTAAACTTGAGACCAATGATGAGTGAAAGGCATATCCTATCAAATTGCATACATGATATGCAAAATTAAGTCTACATTTTAACATGCCTCGCCTATTGTGGACATATTCTTTTATGAATTGTGTTTAACTACTTACTAAAGGTAGCTCTTTTCCTTTGTGTGTGCATGCACNNNNNNNNNNNNNNNNNNNNNNNNNNAGNGNGACNNNNNNNNNNNNNNNNNNNNNNNNAAAAGAACAGNTTTTNAAAAGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=11,311 SEQUENCE_TARGET=240,26 SEQUENCE_TARGET=274,24 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=33.RHAP131FC12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNTTGTATTAGAACACTGAATAACTCTTATCTTTACTCTGTAATTTAAAAATTACATTCTTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCATATATGGAAGTCAGAGGACAATTTGTGGGAAGTTCTCTCTCTATACTGTGTGGGTCCTGAGGAATGAATTTATGTCACCAGGCTTGGTGACCAGCACCTTGACTGGATAATCCACCTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=13,232 SEQUENCE_TARGET=75,44 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=33.RHAP131FC6.seq SEQUENCE_TEMPLATE=NNNNNNNNNNCCGCCTCCAAGGACTCCAAGACTGGATGCACACGCTGAGGCAAGACTGCTGAGGCGACACATGGAGGACACATGATGGTTAGAGGGTATATAATAAGGACTCCACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCACTCGGCTTCCTGGTACACTAGTTGTGTCACACTTGCTGGGCTTTGCTGACCTTTGCTTCACTCAGAGAGGCACAGCTGAAGAACGTCTGATGTTCCTGCTGGTCCCTCCTGCTGACTGGAGCCCGGGCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=10,330 SEQUENCE_TARGET=118,44 SEQUENCE_TARGET=163,40 P3_COMMENT=CL is ACATGGAGGACACATGATGG P3_COMMENT=CR is GTGTACCAGGAAGCCGAGTG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=1.526938 PRIMER_LEFT_0_PENALTY=0.815754 PRIMER_RIGHT_0_PENALTY=0.711184 PRIMER_LEFT_0_SEQUENCE=CATGGAGGACACATGATGGT PRIMER_RIGHT_0_SEQUENCE=GTGTACCAGGAAGCCGAGTG PRIMER_LEFT_0=69,20 PRIMER_RIGHT_0=224,20 PRIMER_LEFT_0_TM=59.184 PRIMER_RIGHT_0_TM=60.711 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=5.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=0.00 PRIMER_LEFT_0_END_STABILITY=7.8000 PRIMER_RIGHT_0_END_STABILITY=6.4000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=156 PRIMER_PAIR_1_PENALTY=1.526938 PRIMER_LEFT_1_PENALTY=0.815754 PRIMER_RIGHT_1_PENALTY=0.711184 PRIMER_LEFT_1_SEQUENCE=ACATGGAGGACACATGATGG PRIMER_RIGHT_1_SEQUENCE=GTGTACCAGGAAGCCGAGTG PRIMER_LEFT_1=68,20 PRIMER_RIGHT_1=224,20 PRIMER_LEFT_1_TM=59.184 PRIMER_RIGHT_1_TM=60.711 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=60.000 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=8.1000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=157 PRIMER_PAIR_2_PENALTY=1.715371 PRIMER_LEFT_2_PENALTY=1.004187 PRIMER_RIGHT_2_PENALTY=0.711184 PRIMER_LEFT_2_SEQUENCE=GACTGCTGAGGCGACACAT PRIMER_RIGHT_2_SEQUENCE=GTGTACCAGGAAGCCGAGTG PRIMER_LEFT_2=53,19 PRIMER_RIGHT_2=224,20 PRIMER_LEFT_2_TM=60.004 PRIMER_RIGHT_2_TM=60.711 PRIMER_LEFT_2_GC_PERCENT=57.895 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.6000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=172 PRIMER_PAIR_3_PENALTY=1.749238 PRIMER_LEFT_3_PENALTY=1.038054 PRIMER_RIGHT_3_PENALTY=0.711184 PRIMER_LEFT_3_SEQUENCE=CGACACATGGAGGACACATG PRIMER_RIGHT_3_SEQUENCE=GTGTACCAGGAAGCCGAGTG PRIMER_LEFT_3=64,20 PRIMER_RIGHT_3=224,20 PRIMER_LEFT_3_TM=61.038 PRIMER_RIGHT_3_TM=60.711 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=6.6000 PRIMER_RIGHT_3_END_STABILITY=6.4000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=161 PRIMER_PAIR_4_PENALTY=1.753105 PRIMER_LEFT_4_PENALTY=1.041921 PRIMER_RIGHT_4_PENALTY=0.711184 PRIMER_LEFT_4_SEQUENCE=AGACTGCTGAGGCGACACAT PRIMER_RIGHT_4_SEQUENCE=GTGTACCAGGAAGCCGAGTG PRIMER_LEFT_4=52,20 PRIMER_RIGHT_4=224,20 PRIMER_LEFT_4_TM=61.042 PRIMER_RIGHT_4_TM=60.711 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=60.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=6.6000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PAIR_4_PRODUCT_SIZE=173 = SEQUENCE_ID=35.RHAP131FD11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNCAGTGGTTAGCAAGCAACACTGAAGAGACTTGTCAGGTTATATTTATATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGCAACAATGATTAAAGAAGAGGAGGCTGTGAAATTTAAGAGCGAGCAGGGGTGGGTATTGAAGAGATTAGAGGGGGAATGTAATTATGTCTTCATTAACTTTTTCAAATTAAAAAAAAAGAATGTGACTTTAATCATATCTAAATCGGAGCAGACTATCAATCCACATACTCCACTCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=25,317 SEQUENCE_TARGET=77,83 P3_COMMENT=CL is CAACACTGAAGAGACTTGTCAGG P3_COMMENT=CR is ACCCCTGCTCGCTCTTAAAT PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=4.180272 PRIMER_LEFT_0_PENALTY=3.951808 PRIMER_RIGHT_0_PENALTY=0.228464 PRIMER_LEFT_0_SEQUENCE=CAACACTGAAGAGACTTGTCAGG PRIMER_RIGHT_0_SEQUENCE=ACCCCTGCTCGCTCTTAAAT PRIMER_LEFT_0=39,23 PRIMER_RIGHT_0=213,20 PRIMER_LEFT_0_TM=59.048 PRIMER_RIGHT_0_TM=60.228 PRIMER_LEFT_0_GC_PERCENT=47.826 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=7.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=5.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=8.2000 PRIMER_RIGHT_0_END_STABILITY=6.2000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=5.00 PRIMER_PAIR_0_PRODUCT_SIZE=175 PRIMER_PAIR_1_PENALTY=4.291559 PRIMER_LEFT_1_PENALTY=1.478935 PRIMER_RIGHT_1_PENALTY=2.812624 PRIMER_LEFT_1_SEQUENCE=TGGTTAGCAAGCAACACTGAA PRIMER_RIGHT_1_SEQUENCE=TTTCACAGCCTCCTCTTCTTT PRIMER_LEFT_1=28,21 PRIMER_RIGHT_1=194,21 PRIMER_LEFT_1_TM=59.521 PRIMER_RIGHT_1_TM=58.187 PRIMER_LEFT_1_GC_PERCENT=42.857 PRIMER_RIGHT_1_GC_PERCENT=42.857 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.0000 PRIMER_RIGHT_1_END_STABILITY=7.0000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PAIR_1_PRODUCT_SIZE=167 PRIMER_PAIR_2_PENALTY=4.557741 PRIMER_LEFT_2_PENALTY=3.855493 PRIMER_RIGHT_2_PENALTY=0.702248 PRIMER_LEFT_2_SEQUENCE=GCAACACTGAAGAGACTTGTCA PRIMER_RIGHT_2_SEQUENCE=CCCCTGCTCGCTCTTAAATT PRIMER_LEFT_2=38,22 PRIMER_RIGHT_2=212,20 PRIMER_LEFT_2_TM=58.145 PRIMER_RIGHT_2_TM=60.702 PRIMER_LEFT_2_GC_PERCENT=45.455 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=5.00 PRIMER_RIGHT_2_SELF_END=4.00 PRIMER_LEFT_2_END_STABILITY=6.7000 PRIMER_RIGHT_2_END_STABILITY=7.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PAIR_2_PRODUCT_SIZE=175 PRIMER_PAIR_3_PENALTY=4.579361 PRIMER_LEFT_3_PENALTY=1.478935 PRIMER_RIGHT_3_PENALTY=3.100425 PRIMER_LEFT_3_SEQUENCE=TGGTTAGCAAGCAACACTGAA PRIMER_RIGHT_3_SEQUENCE=CACAGCCTCCTCTTCTTTAATCA PRIMER_LEFT_3=28,21 PRIMER_RIGHT_3=191,23 PRIMER_LEFT_3_TM=59.521 PRIMER_RIGHT_3_TM=59.900 PRIMER_LEFT_3_GC_PERCENT=42.857 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=7.0000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PAIR_3_PRODUCT_SIZE=164 PRIMER_PAIR_4_PENALTY=4.654056 PRIMER_LEFT_4_PENALTY=3.951808 PRIMER_RIGHT_4_PENALTY=0.702248 PRIMER_LEFT_4_SEQUENCE=CAACACTGAAGAGACTTGTCAGG PRIMER_RIGHT_4_SEQUENCE=CCCCTGCTCGCTCTTAAATT PRIMER_LEFT_4=39,23 PRIMER_RIGHT_4=212,20 PRIMER_LEFT_4_TM=59.048 PRIMER_RIGHT_4_TM=60.702 PRIMER_LEFT_4_GC_PERCENT=47.826 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=7.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=5.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=7.2000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=174 = SEQUENCE_ID=35.RHAP131FD5.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNGNTTAAAATTTCAGTTGTCTTCACTTCACATCCAAGGCTGGATTTCTTGGGATGTGATACAGTGCGGATGGTGCTTGTGGGATCACACAATCTTATGAGACCTGTCTTTCTGTGTTGCCTGTAAACTGTTTCCTAAATANTTTGAGTAGATCTGTTACCTTCTAAATAAGGTTAGATCACTCAAATAACGTATTTGTCTCTAGCTTCTATCTCAGTCACATTTTAATTATTTATACCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGTTTCCATTAACTTTGCCTTNTGANGANGACTCCTTTNTCTTTCANTCTNTTGGGNGGTANCGAAGCTCGNANTCGNNAATNGNNGTCNNNGNTGTTTTCCNGTGTNANNTTNNTNNCCGCNCACNATTCCANNCNNAATANGNNGCGGNAGGATANAAGTGGTANANCNGGGGGNNNNNAAANGNGTTGGGNNANTCAGNNTTANNNGGGGGGNNNNTNNCGNTTNNCAANNNGGGNAANNNNNNNTGNANNNGNNTTAATGNNTNGGGNNANG SEQUENCE_INCLUDED_REGION=11,568 SEQUENCE_TARGET=250,50 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=38.RHAP131FE11.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNCCAATGAGCAACCCTGTCTTAAAACAACTGTGTGTGTTTTTCGGGGGGTCAAGGGGGCTGTTCCTGAGGAATGATTCAAGGTTGATCTCTGGCACCTGTGCTTGTGTGCACACATATGCACAAGTGTATGCACACATACACAACAGAATCTTCCAACACTTTGACCTTGGACTTTCCCTCTTTCAGAAGCAAGAGAAACAGATATCTGTTGTTTAAACCACTTAGTCTACAGTGATTGGTCACTTGGTGTCCTGTGCTATGAGGCGTGTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTGAAATTAGAAACCACCCTTATCACCATGGTTTTCAGAGCCCAGTATCAGTTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=14,402 SEQUENCE_TARGET=286,70 P3_COMMENT=CL is CTGTGCTATGAGGCGTGTGT P3_COMMENT=CR is ACTGGGCTCTGAAAACCATG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.182171 PRIMER_LEFT_0_PENALTY=0.071094 PRIMER_RIGHT_0_PENALTY=0.111077 PRIMER_LEFT_0_SEQUENCE=CTGTGCTATGAGGCGTGTGT PRIMER_RIGHT_0_SEQUENCE=ACTGGGCTCTGAAAACCATG PRIMER_LEFT_0=265,20 PRIMER_RIGHT_0=404,20 PRIMER_LEFT_0_TM=59.929 PRIMER_RIGHT_0_TM=60.111 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=50.000 PRIMER_LEFT_0_SELF_ANY=2.00 PRIMER_RIGHT_0_SELF_ANY=4.00 PRIMER_LEFT_0_SELF_END=0.00 PRIMER_RIGHT_0_SELF_END=4.00 PRIMER_LEFT_0_END_STABILITY=6.4000 PRIMER_RIGHT_0_END_STABILITY=8.4000 PRIMER_PAIR_0_COMPL_ANY=3.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=140 PRIMER_PAIR_1_PENALTY=0.410019 PRIMER_LEFT_1_PENALTY=0.298942 PRIMER_RIGHT_1_PENALTY=0.111077 PRIMER_LEFT_1_SEQUENCE=TGGTGTCCTGTGCTATGAGG PRIMER_RIGHT_1_SEQUENCE=ACTGGGCTCTGAAAACCATG PRIMER_LEFT_1=258,20 PRIMER_RIGHT_1=404,20 PRIMER_LEFT_1_TM=59.701 PRIMER_RIGHT_1_TM=60.111 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=4.00 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=147 PRIMER_PAIR_2_PENALTY=0.544789 PRIMER_LEFT_2_PENALTY=0.071094 PRIMER_RIGHT_2_PENALTY=0.473695 PRIMER_LEFT_2_SEQUENCE=CTGTGCTATGAGGCGTGTGT PRIMER_RIGHT_2_SEQUENCE=CCATGGTGATAAGGGTGGTT PRIMER_LEFT_2=265,20 PRIMER_RIGHT_2=389,20 PRIMER_LEFT_2_TM=59.929 PRIMER_RIGHT_2_TM=59.526 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=2.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=125 PRIMER_PAIR_3_PENALTY=0.544789 PRIMER_LEFT_3_PENALTY=0.071094 PRIMER_RIGHT_3_PENALTY=0.473695 PRIMER_LEFT_3_SEQUENCE=CTGTGCTATGAGGCGTGTGT PRIMER_RIGHT_3_SEQUENCE=AACCATGGTGATAAGGGTGG PRIMER_LEFT_3=265,20 PRIMER_RIGHT_3=391,20 PRIMER_LEFT_3_TM=59.929 PRIMER_RIGHT_3_TM=59.526 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=2.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=127 PRIMER_PAIR_4_PENALTY=0.737696 PRIMER_LEFT_4_PENALTY=0.626619 PRIMER_RIGHT_4_PENALTY=0.111077 PRIMER_LEFT_4_SEQUENCE=TGGTCACTTGGTGTCCTGTG PRIMER_RIGHT_4_SEQUENCE=ACTGGGCTCTGAAAACCATG PRIMER_LEFT_4=250,20 PRIMER_RIGHT_4=404,20 PRIMER_LEFT_4_TM=60.627 PRIMER_RIGHT_4_TM=60.111 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=1.00 PRIMER_RIGHT_4_SELF_END=4.00 PRIMER_LEFT_4_END_STABILITY=6.7000 PRIMER_RIGHT_4_END_STABILITY=8.4000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=155 = SEQUENCE_ID=38.RHAP131FE5.seq SEQUENCE_TEMPLATE=NNNNNNNNNCANCTCCGAAAAAAANNCCAAAACTAGTTCTCAGCTCTAGATAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCCTTACCTTGATCCTGACGCCCTTGTGTCATTTGTAGGTCAACCAACCACCCATACGTTTCAAGCAATAAAGAATCCAGAGAAAATAATCACNNNNNNNNNNNNNNNNNNGAGTTTTCTGACAGATGCCCAAAGATGCTTTCCAAAGCGTATGTTAGAAAGTGTGAACGAATGAATTCCTCTCTCCCTTCCCGCCCTAAAACACGAACTCACATTCTCCCAGAGATCCACTCCACTCTCCTGTTCCTTCTGTGATAGTTAATTTTGTCTCTTAGCATCACTAGATTTCCAGTCACCTAAGAAACATACCCCTGGGTGTGTGTGTCTTTTGGGGTGATCCTGCAAAAGGTTTAACTGAGGAGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=9,456 SEQUENCE_TARGET=59,32 SEQUENCE_TARGET=190,18 P3_COMMENT=CL is CTTACCTTGATCCTGACGCC P3_COMMENT=CR is GCTTTGGAAAGCATCTTTGG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.483158 PRIMER_LEFT_0_PENALTY=0.306150 PRIMER_RIGHT_0_PENALTY=0.177008 PRIMER_LEFT_0_SEQUENCE=CTTACCTTGATCCTGACGCC PRIMER_RIGHT_0_SEQUENCE=GCTTTGGAAAGCATCTTTGG PRIMER_LEFT_0=99,20 PRIMER_RIGHT_0=245,20 PRIMER_LEFT_0_TM=59.694 PRIMER_RIGHT_0_TM=59.823 PRIMER_LEFT_0_GC_PERCENT=55.000 PRIMER_RIGHT_0_GC_PERCENT=45.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=8.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=11.1000 PRIMER_RIGHT_0_END_STABILITY=8.8000 PRIMER_PAIR_0_COMPL_ANY=4.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=147 PRIMER_PAIR_1_PENALTY=0.483158 PRIMER_LEFT_1_PENALTY=0.306150 PRIMER_RIGHT_1_PENALTY=0.177008 PRIMER_LEFT_1_SEQUENCE=CCTTACCTTGATCCTGACGC PRIMER_RIGHT_1_SEQUENCE=GCTTTGGAAAGCATCTTTGG PRIMER_LEFT_1=98,20 PRIMER_RIGHT_1=245,20 PRIMER_LEFT_1_TM=59.694 PRIMER_RIGHT_1_TM=59.823 PRIMER_LEFT_1_GC_PERCENT=55.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=8.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=8.8000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=148 PRIMER_PAIR_2_PENALTY=0.483158 PRIMER_LEFT_2_PENALTY=0.306150 PRIMER_RIGHT_2_PENALTY=0.177008 PRIMER_LEFT_2_SEQUENCE=GCCTTACCTTGATCCTGACG PRIMER_RIGHT_2_SEQUENCE=GCTTTGGAAAGCATCTTTGG PRIMER_LEFT_2=97,20 PRIMER_RIGHT_2=245,20 PRIMER_LEFT_2_TM=59.694 PRIMER_RIGHT_2_TM=59.823 PRIMER_LEFT_2_GC_PERCENT=55.000 PRIMER_RIGHT_2_GC_PERCENT=45.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=8.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=8.4000 PRIMER_RIGHT_2_END_STABILITY=8.8000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=149 PRIMER_PAIR_3_PENALTY=0.489018 PRIMER_LEFT_3_PENALTY=0.312010 PRIMER_RIGHT_3_PENALTY=0.177008 PRIMER_LEFT_3_SEQUENCE=CCTGCCTTACCTTGATCCTG PRIMER_RIGHT_3_SEQUENCE=GCTTTGGAAAGCATCTTTGG PRIMER_LEFT_3=94,20 PRIMER_RIGHT_3=245,20 PRIMER_LEFT_3_TM=59.688 PRIMER_RIGHT_3_TM=59.823 PRIMER_LEFT_3_GC_PERCENT=55.000 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=8.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=8.8000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=152 PRIMER_PAIR_4_PENALTY=0.519655 PRIMER_LEFT_4_PENALTY=0.306150 PRIMER_RIGHT_4_PENALTY=0.213506 PRIMER_LEFT_4_SEQUENCE=CTTACCTTGATCCTGACGCC PRIMER_RIGHT_4_SEQUENCE=AAAGCATCTTTGGGCATCTG PRIMER_LEFT_4=99,20 PRIMER_RIGHT_4=238,20 PRIMER_LEFT_4_TM=59.694 PRIMER_RIGHT_4_TM=60.214 PRIMER_LEFT_4_GC_PERCENT=55.000 PRIMER_RIGHT_4_GC_PERCENT=45.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=11.1000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=140 = SEQUENCE_ID=40.RHAP131FF4.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNCAGTCACACAAAGATAGAGTATAGAATAGAGTTTATTTAGGGCATGGGGAGGAGAGTTGAAAGGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGGAGTAGAGGCTGGTGGTGAGCACGTAGAGAGAAGGGAAGGGGCAGGGGAAGGAGAGAAAGGGAGAGCAGGAACAAGAGAGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=20,212 SEQUENCE_TARGET=86,58 P3_COMMENT=CL is CATGGGGAGGAGAGTTGAAA P3_COMMENT=CR is CCCCTTCCCTTCTCTCTACG PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.238868 PRIMER_LEFT_0_PENALTY=0.042377 PRIMER_RIGHT_0_PENALTY=0.196491 PRIMER_LEFT_0_SEQUENCE=CATGGGGAGGAGAGTTGAAA PRIMER_RIGHT_0_SEQUENCE=CCCCTTCCCTTCTCTCTACG PRIMER_LEFT_0=62,20 PRIMER_RIGHT_0=189,20 PRIMER_LEFT_0_TM=60.042 PRIMER_RIGHT_0_TM=60.196 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=60.000 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=2.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=7.3000 PRIMER_RIGHT_0_END_STABILITY=7.4000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=2.00 PRIMER_PAIR_0_PRODUCT_SIZE=128 PRIMER_PAIR_1_PENALTY=0.293572 PRIMER_LEFT_1_PENALTY=0.042377 PRIMER_RIGHT_1_PENALTY=0.251196 PRIMER_LEFT_1_SEQUENCE=CATGGGGAGGAGAGTTGAAA PRIMER_RIGHT_1_SEQUENCE=TCTCCCTTTCTCTCCTTCCC PRIMER_LEFT_1=62,20 PRIMER_RIGHT_1=212,20 PRIMER_LEFT_1_TM=60.042 PRIMER_RIGHT_1_TM=59.749 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=0.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_END_STABILITY=7.3000 PRIMER_RIGHT_1_END_STABILITY=9.7000 PRIMER_PAIR_1_COMPL_ANY=9.00 PRIMER_PAIR_1_COMPL_END=6.00 PRIMER_PAIR_1_PRODUCT_SIZE=151 PRIMER_PAIR_2_PENALTY=0.510463 PRIMER_LEFT_2_PENALTY=0.042377 PRIMER_RIGHT_2_PENALTY=0.468086 PRIMER_LEFT_2_SEQUENCE=CATGGGGAGGAGAGTTGAAA PRIMER_RIGHT_2_SEQUENCE=CCTGCTCTCCCTTTCTCTCC PRIMER_LEFT_2=62,20 PRIMER_RIGHT_2=217,20 PRIMER_LEFT_2_TM=60.042 PRIMER_RIGHT_2_TM=60.468 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=60.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_END_STABILITY=7.3000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=4.00 PRIMER_PAIR_2_PRODUCT_SIZE=156 PRIMER_PAIR_3_PENALTY=0.512377 PRIMER_LEFT_3_PENALTY=0.042377 PRIMER_RIGHT_3_PENALTY=0.470001 PRIMER_LEFT_3_SEQUENCE=CATGGGGAGGAGAGTTGAAA PRIMER_RIGHT_3_SEQUENCE=TCTCTTGTTCCTGCTCTCCC PRIMER_LEFT_3=62,20 PRIMER_RIGHT_3=226,20 PRIMER_LEFT_3_TM=60.042 PRIMER_RIGHT_3_TM=59.530 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_END_STABILITY=7.3000 PRIMER_RIGHT_3_END_STABILITY=9.4000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=5.00 PRIMER_PAIR_3_PRODUCT_SIZE=165 PRIMER_PAIR_4_PENALTY=0.637090 PRIMER_LEFT_4_PENALTY=0.042377 PRIMER_RIGHT_4_PENALTY=0.594713 PRIMER_LEFT_4_SEQUENCE=CATGGGGAGGAGAGTTGAAA PRIMER_RIGHT_4_SEQUENCE=TTGTTCCTGCTCTCCCTTTC PRIMER_LEFT_4=62,20 PRIMER_RIGHT_4=222,20 PRIMER_LEFT_4_TM=60.042 PRIMER_RIGHT_4_TM=59.405 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_END_STABILITY=7.3000 PRIMER_RIGHT_4_END_STABILITY=7.0000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=4.00 PRIMER_PAIR_4_PRODUCT_SIZE=161 = SEQUENCE_ID=43.RHAP131FG10.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNCCATACACAGAACTAGGGTCTATACACAGTACTAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTACAGACATGAATGCATAGTGTAGGCATAAAAAGAAAGGCTCGATTTCATTCTTTTATCCACACTGTTCACCTGTGTCACCTGTTTTGAGCATGTGAGTCTGTTTTGAGCATGCTAGCCTGTATATAACTAGAGGAACTTAACAAGACTGCACAAGTGAACTCAGGGTAAGGACATATATAAAAGAACATTACAATAGGCAGGGTGAAGTGGGAACAGCAATCCCAGAACACATTCTGGAAATGTTGTAGTTTGAGGAGGAAGAGAGGGAAAGGTCACTCTGGGCATAATTTGGCTTTAACATCAATTTTCCTTCTGGTAGGAGTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=23,414 SEQUENCE_TARGET=60,46 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=47.RHAP131FH5.seq SEQUENCE_TEMPLATE=ANNTNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGTTNGTGTCTGGGGGGAGGCTNCTCTCCTGTGTGNTGCAGCCANTGGGGTTGGGGAGGAGCANANAGTTTGCNTTNNTGGAGTGAATCAACCAAATTTTGGGAGTTGGAAGTATTTTATTANAAACATNTGGAGATTTTAGAATTTTTTNAAAATGTTGTTATTTAAANCCTTTTCAACGTTAGAAAGTAATAAGTAGGGGTGGGNGGNACGCCCTCNNAGGNNNTTGTTTCAAAANGNNTCNCCCAACTCCCATGANTTTCTTGGNTTTTTTTNNTTNCCGGNAACCNTGNTCTCCTNNAANATCAAAACCCATCAACTTTTGGNGGCCANAATAATCNNTTTTTACTANANTGNNTGNGNGTCCCTTGGTANANTNGNTTNNGGGGGTANCNGANCTCNANNTTCNNTAANTNATGGGNNAAANNGGTTTTTTTNCNTGGGGNNNANTTGTTTTNNTCTNNCCNNNNATTTTTNCNACATTGANANNNNGGNNCCCGGGGCGCNATTNNNGGTGTAANNNCCTGGGGGNNNNNTAANNGNGNGGNNNNNNCCTCANTTTTTANTGGGCGGTNNNCCNCNNNTGCCCNTTTTTTCTGNNTGGGGAAANNTTGTTNNNNNNNNTNG SEQUENCE_INCLUDED_REGION=0,663 SEQUENCE_TARGET=6,29 P3_COMMENT=CL is P3_COMMENT=CR is PRIMER_LEFT_NUM_RETURNED=0 PRIMER_RIGHT_NUM_RETURNED=0 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=0 = SEQUENCE_ID=48.RHAP131FH12.seq SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNCAGGCATGNGTGAGGGGATGAGAACCCACCTGCAATGTNAAGGAAGTGATGTGATTATTGTTCATGCCCATGTCTCATTCTTCTCCCTTTCCCATGTTGGTCCTGATGGAAATGGAAAAAATAGAGTTGGGGGAAACTTGGAGACCTTATTGTAATATTAGAGACTATGAGGAATCTTAGGATTCTTTTGGGAAAGGCTTGGGCATGTCTTATGATAGATTTCAAGCTAGTAACAACACATCCAAGGATGCTTCTTAAGAAATTTAATTAATAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATTCAGGTCAACATAGTTGCTTGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN SEQUENCE_INCLUDED_REGION=21,348 SEQUENCE_TARGET=297,40 P3_COMMENT=CL is AGGCTTGGGCATGTCTTATG P3_COMMENT=CR is TCCACAAGCAACTATGTTGACC PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=2.133110 PRIMER_LEFT_0_PENALTY=0.095771 PRIMER_RIGHT_0_PENALTY=2.037339 PRIMER_LEFT_0_SEQUENCE=AGGCTTGGGCATGTCTTATG PRIMER_RIGHT_0_SEQUENCE=TCCACAAGCAACTATGTTGACC PRIMER_LEFT_0=215,20 PRIMER_RIGHT_0=366,22 PRIMER_LEFT_0_TM=60.096 PRIMER_RIGHT_0_TM=60.037 PRIMER_LEFT_0_GC_PERCENT=50.000 PRIMER_RIGHT_0_GC_PERCENT=45.455 PRIMER_LEFT_0_SELF_ANY=4.00 PRIMER_RIGHT_0_SELF_ANY=6.00 PRIMER_LEFT_0_SELF_END=2.00 PRIMER_RIGHT_0_SELF_END=2.00 PRIMER_LEFT_0_END_STABILITY=6.2000 PRIMER_RIGHT_0_END_STABILITY=7.9000 PRIMER_PAIR_0_COMPL_ANY=5.00 PRIMER_PAIR_0_COMPL_END=1.00 PRIMER_PAIR_0_PRODUCT_SIZE=152 PRIMER_PAIR_1_PENALTY=2.263884 PRIMER_LEFT_1_PENALTY=0.095771 PRIMER_RIGHT_1_PENALTY=2.168114 PRIMER_LEFT_1_SEQUENCE=AGGCTTGGGCATGTCTTATG PRIMER_RIGHT_1_SEQUENCE=CCTCCACAAGCAACTATGTTGA PRIMER_LEFT_1=215,20 PRIMER_RIGHT_1=368,22 PRIMER_LEFT_1_TM=60.096 PRIMER_RIGHT_1_TM=60.168 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.455 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=5.00 PRIMER_LEFT_1_END_STABILITY=6.2000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PAIR_1_PRODUCT_SIZE=154 PRIMER_PAIR_2_PENALTY=2.329753 PRIMER_LEFT_2_PENALTY=0.292414 PRIMER_RIGHT_2_PENALTY=2.037339 PRIMER_LEFT_2_SEQUENCE=AAAGGCTTGGGCATGTCTTA PRIMER_RIGHT_2_SEQUENCE=TCCACAAGCAACTATGTTGACC PRIMER_LEFT_2=213,20 PRIMER_RIGHT_2=366,22 PRIMER_LEFT_2_TM=59.708 PRIMER_RIGHT_2_TM=60.037 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=4.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_END_STABILITY=6.0000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=154 PRIMER_PAIR_3_PENALTY=2.460528 PRIMER_LEFT_3_PENALTY=0.292414 PRIMER_RIGHT_3_PENALTY=2.168114 PRIMER_LEFT_3_SEQUENCE=AAAGGCTTGGGCATGTCTTA PRIMER_RIGHT_3_SEQUENCE=CCTCCACAAGCAACTATGTTGA PRIMER_LEFT_3=213,20 PRIMER_RIGHT_3=368,22 PRIMER_LEFT_3_TM=59.708 PRIMER_RIGHT_3_TM=60.168 PRIMER_LEFT_3_GC_PERCENT=45.000 PRIMER_RIGHT_3_GC_PERCENT=45.455 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=4.00 PRIMER_RIGHT_3_SELF_END=5.00 PRIMER_LEFT_3_END_STABILITY=6.0000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PAIR_3_PRODUCT_SIZE=156 PRIMER_PAIR_4_PENALTY=2.505221 PRIMER_LEFT_4_PENALTY=0.467882 PRIMER_RIGHT_4_PENALTY=2.037339 PRIMER_LEFT_4_SEQUENCE=GGATTCTTTTGGGAAAGGCT PRIMER_RIGHT_4_SEQUENCE=TCCACAAGCAACTATGTTGACC PRIMER_LEFT_4=200,20 PRIMER_RIGHT_4=366,22 PRIMER_LEFT_4_TM=59.532 PRIMER_RIGHT_4_TM=60.037 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=45.455 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_END_STABILITY=9.4000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PAIR_4_PRODUCT_SIZE=167 = primer3-2.3.6/test/primer_boundary_input0000664000077300007730000013023112231156245017400 0ustar ioanaioanaPRIMER_TM_FORMULA=0 PRIMER_SALT_CORRECTIONS=0 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=0 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0 PRIMER_SALT_DIVALENT=0.0 PRIMER_DNTP_CONC=0.0 PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1 SEQUENCE_ID=boundary_test_00 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MAX_END_STABILITY=100.0 = SEQUENCE_ID=boundary_test_01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-100 = SEQUENCE_ID=boundary_test_02 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 = SEQUENCE_ID=boundary_test_03 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=56-100 P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_04 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=50-54 P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_05 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MIN_SIZE=24 PRIMER_MAX_SIZE=24 PRIMER_OPT_SIZE=24 = SEQUENCE_ID=boundary_test_06 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MIN_SIZE=26 PRIMER_MAX_SIZE=26 PRIMER_OPT_SIZE=26 P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_07 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 SEQUENCE_INCLUDED_REGION=2,55 = SEQUENCE_ID=boundary_test_08 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 SEQUENCE_INCLUDED_REGION=3,55 P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_09 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=50-100 SEQUENCE_INCLUDED_REGION=2,54 P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_10 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCTNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_11 PRIMER_MAX_NS_ACCEPTED=1 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_12 PRIMER_MAX_NS_ACCEPTED=2 SEQUENCE_TEMPLATE=NNCACGTCATCNNATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_13 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_14 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=.0001 SEQUENCE_TEMPLATE=NNAAAAAAAAAAAAAAAAAAAAAAAAANNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_15 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=20 PRIMER_MAX_GC=100 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_16 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=.0001 SEQUENCE_TEMPLATE=NNGGGGGGGGGGGGGGGGGGGGGGGGGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_17 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_18 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=3,55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_19 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,54 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_20 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=54-55 SEQUENCE_INCLUDED_REGION=2,54 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_21 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_ID=boundary_test_22 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=26,6 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_23 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,6 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_24 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,1 SEQUENCE_TARGET=28,4 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_26 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=26,1 SEQUENCE_TARGET=27,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_27 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,1 SEQUENCE_TARGET=28,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_28 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,1 SEQUENCE_TARGET=29,4 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_29 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_30 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=28,0 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_31 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=0,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_32 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_33 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=26,6 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_34 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,6 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_35 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_36 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,1 SEQUENCE_EXCLUDED_REGION=28,4 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_37 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=26,1 SEQUENCE_EXCLUDED_REGION=27,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_37 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,1 SEQUENCE_EXCLUDED_REGION=28,5 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_38 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 SEQUENCE_EXCLUDED_REGION=29,4 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_39 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_40 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,0 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_41 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=10,0 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN = SEQUENCE_ID=boundary_test_42 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_43 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=9.99 PRIMER_PAIR_MAX_COMPL_END=9.99 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because one primer has end compl = SEQUENCE_ID=boundary_test_44 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=10.01 PRIMER_PAIR_MAX_COMPL_END=10.01 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_45 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=12.01 PRIMER_PAIR_MAX_COMPL_ANY=12.01 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_46 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=11.99 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_PAIR_MAX_COMPL_ANY=11.99 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because self any for one primer is 12.00" = SEQUENCE_ID=boundary_test_47 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_48 PRIMER_GC_CLAMP=1 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because of GC clamp)" = SEQUENCE_ID=boundary_test_49 PRIMER_GC_CLAMP=1 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_50 PRIMER_GC_CLAMP=2 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (because of GC clamp)" = SEQUENCE_ID=boundary_test_51 PRIMER_PAIR_MAX_DIFF_TM=100 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_52 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_53 PRIMER_PAIR_MAX_DIFF_TM=0.28 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should fail (due to max diff tm)" = SEQUENCE_ID=boundary_test_54 PRIMER_LIBERAL_BASE=1 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should succeed" = SEQUENCE_ID=boundary_test_55 PRIMER_LIBERAL_BASE=0 PRIMER_PAIR_MAX_DIFF_TM=0.29 PRIMER_GC_CLAMP=0 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=28,1 PRIMER_PRODUCT_SIZE_RANGE="55-55" PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=25 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNKNNCCTCACTGTCATTTCCAAACAATTCNN P3_COMMENT="should generate error" = SEQUENCE_ID=poly_x_1 SEQUENCE_TEMPLATE=AAAAAATTTTTTGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGTTTTTTCCCCCCAAAAAAGGGGGGCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTAAAAAAGGGGGGCCCCCCAAAAAAGGGGGGTTTTTTTCCCCCC PRIMER_MAX_POLY_X=5 PRIMER_INTERNAL_MAX_POLY_X=6 PRIMER_PRODUCT_SIZE_RANGE=50-300 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PAIR_MAX_DIFF_TM=100 PRIMER_MAX_SELF_ANY=100 PRIMER_MAX_SELF_END=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 = SEQUENCE_ID=poly_x_2 PRIMER_INTERNAL_MIN_TM=0 PRIMER_INTERNAL_MAX_TM=100 SEQUENCE_TEMPLATE=AAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGGAAAAATTTTTCCCCCGGGGGAAAAACCCCCTTTTTGGGGGAAAAACCCCCTTTTTGGGGG = SEQUENCE_ID=poly_x_3 SEQUENCE_TEMPLATE=AAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTGGGGGGAAAAAAACCCCCCTTTTTTGGGGGGAAAAAACCCCCCTTTTTTTGGGGGGGGGGGGGG PRIMER_MAX_POLY_X=6 PRIMER_INTERNAL_MAX_POLY_X=5 = SEQUENCE_ID=boundary_test_59 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=27,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=ok (excluded region is between G and N and end of left primer). = SEQUENCE_ID=boundary_test_60 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=26,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (excluded region is between T and G at end of left primer). = SEQUENCE_ID=boundary_test_61 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_EXCLUDED_REGION=26,-1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (negative excluded region size). = SEQUENCE_ID=boundary_test_62 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=1,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED REGION = SEQUENCE_ID=boundary_test_63 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=2,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers = SEQUENCE_ID=boundary_test_64 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=59,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with EXCLUDED_REGION outside of sequence = SEQUENCE_ID=boundary_test_65 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=58,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with PRIMER_WARNING EXCLUDED_REGION outside of INCLUDED_REGION = SEQUENCE_ID=boundary_test_66 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=56,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers = SEQUENCE_ID=boundary_test_67 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_EXCLUDED_REGION=56,2 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to pick primers and warn EXCLUDED_REGION outside of INCLUDED_REGION = SEQUENCE_ID=boundary_test_68 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,0 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=ok (TARGET is between G and N and end of left primer). = SEQUENCE_ID=boundary_test_69 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=26,0 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (TARGET is between T and G at end of left primer). = SEQUENCE_ID=boundary_test_70 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=26,-1 PRIMER_PRODUCT_SIZE_RANGE="55-55" SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (negative TARGET size). = SEQUENCE_ID=boundary_test_71 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=1,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to find legal primers, and warn TARGET outside of INCLUDED REGION = SEQUENCE_ID=boundary_test_72 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=2,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers = SEQUENCE_ID=boundary_test_73 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=59,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with TARGET outside of sequence = SEQUENCE_ID=boundary_test_74 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=58,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to find primers (none spans target), and warn TARGET outside of INCLUDED_REGION = SEQUENCE_ID=boundary_test_75 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=56,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail with no legal primers = SEQUENCE_ID=boundary_test_76 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=56,2 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail to pick primers, and warn TARGET outside of INCLUDED_REGION = SEQUENCE_ID=boundary_test_77 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=27,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (target is at TTG^NNN) = SEQUENCE_ID=boundary_test_78 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=27,5 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (target is the middle run of N) = SEQUENCE_ID=boundary_test_79_1 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=27,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_80 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=31,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_81 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=32,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_82 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=0,59 SEQUENCE_TARGET=32,0 0,1 58,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_83 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=0,59 SEQUENCE_TARGET=0,1 58,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (no target) = SEQUENCE_ID=boundary_test_84 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=0,59 SEQUENCE_TARGET=0,1 32,0 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_85 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=0,59 SEQUENCE_TARGET=32,0 58,1 PRIMER_PRODUCT_SIZE_RANGE=50-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_86 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=35 PRIMER_MAX_SIZE=35 PRIMER_OPT_SIZE=35 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_PRODUCT_SIZE_RANGE=75-75 SEQUENCE_TEMPLATE=NNCACGTCATCTATATATATATCATCAAATTTGATTGNNNNNACTCATATATATATACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed (testing hard limit MAX_PRIMER_LENGTH) = SEQUENCE_ID=boundary_test_71_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 SEQUENCE_TARGET=1,1 27,1 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should warn TARGET outside of INCLUDED REGION = SEQUENCE_ID=boundary_test_72_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,56 PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one pair = SEQUENCE_ID=boundary_test_73_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,56 PRIMER_PRODUCT_SIZE_RANGE=54-54 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should fail (no primers with acceptable product size) = SEQUENCE_ID=boundary_test_74_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,56 PRIMER_PRODUCT_SIZE_RANGE=54-55 55-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair = SEQUENCE_ID=boundary_test_75_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=54-54 55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair = SEQUENCE_ID=boundary_test_76_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,56 PRIMER_PRODUCT_SIZE_RANGE=55-55 56-56 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly one primer pair = SEQUENCE_ID=boundary_test_77_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,82 PRIMER_PRODUCT_SIZE_RANGE=55-55 82-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product second = SEQUENCE_ID=boundary_test_78_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,82 PRIMER_PRODUCT_SIZE_RANGE=82-82 55-55 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product first = SEQUENCE_ID=boundary_test_79_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,82 PRIMER_PRODUCT_SIZE_RANGE=55-55 55-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product second; PRIMER_PAIR_EXPLAIN will be incorrect (too many pairs ok) because the PRIMER_PRODUCT_SIZE_RANGE's overlap = SEQUENCE_ID=boundary_test_80_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,82 PRIMER_PRODUCT_SIZE_RANGE=82-82 55-82 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNNACTCACTGTCATTTCCAAACAATTCNN P3_COMMENT=should succeed with exactly 2 primer pairs, biggest product first = SEQUENCE_ID=boundary_test_81_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing pairwise complementarity checking) = SEQUENCE_ID=boundary_test_82_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=10.99 PRIMER_PAIR_MAX_COMPL_ANY=10.99 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (self any for pairs) = SEQUENCE_ID=boundary_test_83_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing pairwise complementarity checking) = SEQUENCE_ID=boundary_test_84_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=9.99 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=9.99 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (self end for pairs) = SEQUENCE_ID=boundary_test_85_2 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 55-55 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of product size ranges) = SEQUENCE_ID=boundary_test_86 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TARGET=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of targets) = SEQUENCE_ID=boundary_test_87 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_TARGET=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (testing number of targets) = SEQUENCE_ID=boundary_test_88 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_EXCLUDED_REGION=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should succeed (testing number of excluded regions) = SEQUENCE_ID=boundary_test_89 PRIMER_PICK_INTERNAL_OLIGO=0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_INCLUDED_REGION=2,55 PRIMER_PRODUCT_SIZE_RANGE=55-55 SEQUENCE_EXCLUDED_REGION=27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 27,1 PRIMER_MAX_SELF_ANY=11 PRIMER_MAX_SELF_END=10 PRIMER_PAIR_MAX_COMPL_ANY=11 PRIMER_PAIR_MAX_COMPL_END=10 SEQUENCE_TEMPLATE=NNAATGTGATTGCATCAAATGTGATTGNNNNNAATGTGATTGTCATTTCCACAATTCNN P3_COMMENT=should fail (testing number of excluded regions) = SEQUENCE_ID=boundary_test_90 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MAX_END_STABILITY=6.9 P3_COMMENT=should succeed = SEQUENCE_ID=boundary_test_91 SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCNN PRIMER_EXPLAIN_FLAG=1 PRIMER_MAX_POLY_X=0 PRIMER_INTERNAL_MAX_POLY_X=0 PRIMER_PRODUCT_SIZE_RANGE=50-100 PRIMER_MIN_TM=0.0 PRIMER_MAX_TM=100.0 PRIMER_MIN_SIZE=25 PRIMER_MAX_SIZE=25 PRIMER_OPT_SIZE=25 PRIMER_MAX_GC=100 PRIMER_MIN_GC=0 PRIMER_MAX_SELF_END=100 PRIMER_MAX_SELF_ANY=100 PRIMER_PAIR_MAX_COMPL_ANY=100 PRIMER_PAIR_MAX_COMPL_END=100 PRIMER_MAX_END_STABILITY=6.8999 P3_COMMENT=should fail = SEQUENCE_ID=boundary_test_92 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,1 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=100 SEQUENCE_TEMPLATE=ATCACGTCATCTCATCAAATTTGATTGNNNNNACTCACTGTCATTTCCAAACAATTCGG = SEQUENCE_ID=boundary_test_93 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,1 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=100 SEQUENCE_TEMPLATE=ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG = SEQUENCE_ID=boundary_test_94 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=100 SEQUENCE_TEMPLATE=ATCACGTCATCTCATCAAATTTGATNGNCGATACTCACTGTCATTTCCAAACAATTCGG = SEQUENCE_ID=boundary_test_95 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,10 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=0 PRIMER_INSIDE_PENALTY=1000 SEQUENCE_TEMPLATE=ATCACGTCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATNCGG = SEQUENCE_ID=boundary_test_96 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 SEQUENCE_TARGET=27,10 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=0 PRIMER_INSIDE_PENALTY=1000 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG = SEQUENCE_ID=boundary_test_97 PRIMER_MAX_NS_ACCEPTED=0 PRIMER_MIN_GC=0 PRIMER_MAX_GC=100 PRIMER_MAX_END_STABILITY=100 PRIMER_PRODUCT_SIZE_RANGE=55-55 PRIMER_OUTSIDE_PENALTY=0 PRIMER_INSIDE_PENALTY=1000 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG = SEQUENCE_ID=test_098 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATTTTACTCACTGTCATTTCCAAACAATACGG PRIMER_INSIDE_PENALTY=-1.0 PRIMER_PRODUCT_MAX_TM=62.4120 P3_COMMENT=Should succeed. = SEQUENCE_ID=test_099 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_PRODUCT_MAX_TM=62.4119 P3_COMMENT=Should fail. = SEQUENCE_ID=test_100 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_PRODUCT_MAX_TM=1000000 P3_COMMENT=Make sure that product tm output gets turned off again. = SEQUENCE_ID=test_101 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_PRODUCT_MIN_TM=62.4119 P3_COMMENT=Should succeed. = SEQUENCE_ID=test_102 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_PRODUCT_MIN_TM=63.9030 P3_COMMENT=Should fail. = SEQUENCE_ID=test_103 SEQUENCE_TEMPLATE=ATCNCATCATCTCATCAAATTTGATTGATCGTACTCACTGTCATTTCCAAACAATACGG PRIMER_PRODUCT_MIN_TM=-1000000 P3_COMMENT=Make sure that product tm output gets turned off again. =